LOCUS       DAXLDT010000001        75711 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXLDT010000001 DAXLDT010000000
VERSION     DAXLDT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 75711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75711
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.001"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            218..1315
                     /locus_tag="ACF2SP_000001"
     CDS             218..1315
                     /locus_tag="ACF2SP_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFD2357818.1"
                     /translation="MTIAILSTGDEIIHGDTLNTNAHYIAHALCSEGLPLGLQVACSD
                     KENEIIDSLNFLTEHHDILILIGGLGPTKDDITRFALAKFTKEPLIQHADALAHIEKK
                     ANIAKISLNQGNLQQCLFPANARLFPNPYGTAMGCSYSWKGKVFILLPGPPRECLPMF
                     NQDVLPLLLQTSRHSNKQILRWRVFGLAESEIAQILEEALEGLDCQTGYRLETPYLEF
                     KVRCKRELIEKITSIIDPILAPHIISSLETKASDQLRSLILKNNEPITIIDEITGGLL
                     QSLLVRPENYHLLNFHNLNRTKLYFHCSGLEDYWSQQAPQGTTKLIINYSNQMQEGRE
                     THQIPYRSAMVVHYAAEWLSFRLFHLINQLH"
     gene            complement(1274..2299)
                     /gene="cgtA"
                     /locus_tag="ACF2SP_000002"
     CDS             complement(1274..2299)
                     /gene="cgtA"
                     /locus_tag="ACF2SP_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFD2357819.1"
                     /translation="MKFVDEALIKVEAGKGGNGCLSFRREKFIPRGGPDGGDGGDGGS
                     IYFEASSDLNTLIDFRYTRQYKAENGQSGMGGNCTGKKGEDLTIKVPVGTMVYDADTG
                     ELLADISQPGVPVLIAQGGFHGLGNTRYKSSVNRSPRQTTPGSPGESRNLRLELRVLA
                     DVGLLGLPNAGKSTLIRAVSSSKAKVADYPFTTLHPGLGVVRVSPYKSFVMADIPGLI
                     EGAAQGAGLGHRFLKHLSRTCVLLHVIDIAPLDGSDPVADAKAILNELTQYNPDLLNK
                     PRWLVLNKIDMLPDEKEREEKIQSIIKGLKWKDKVFSISAIESKGTQELCYALMQLID
                     EMKESEA"
     gene            complement(2513..2791)
                     /gene="rpmA"
                     /locus_tag="ACF2SP_000003"
     CDS             complement(2513..2791)
                     /gene="rpmA"
                     /locus_tag="ACF2SP_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFD2357820.1"
                     /translation="MAHKKAGGSTRNGRDSNPKYLGVKRFGGQFVNAGEIIVRQRGTR
                     FHPGPGVGCGRDHTLYALVEGLVQFTTKGEKNRKYVTILPEQREEAAS"
     gene            complement(2804..3115)
                     /gene="rplU"
                     /locus_tag="ACF2SP_000004"
     CDS             complement(2804..3115)
                     /gene="rplU"
                     /locus_tag="ACF2SP_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFD2357821.1"
                     /translation="MYAVIKTGGKQYTVKEGDVLKIEMLPENVGNEIKFSEVLMLVDG
                     DKVTCGTPFVAKATVKAEVLDHGRHKKVKIIKFRRRKHHMKQMGHRQYYSQVKITAIG
                     K"
     gene            3358..4017
                     /locus_tag="ACF2SP_000005"
     CDS             3358..4017
                     /locus_tag="ACF2SP_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFD2357822.1"
                     /translation="MSTIQLEAQSRTDMGKGASRRLRRLENKVPAVIYGGSKKPMAIH
                     FSHNKVIKALETESIYSSVFDITVDGKVEHVILKALQRHPYKPIVLHMDLQRVSSKDI
                     LVKLVPVHFINEEQSPGIKAGGIVQHTMTQVEIRCQAKDLPEFIEVDMSKVGMDDVVH
                     LSDLKLPKGVQLTVDVADGSHDAPVVSIHAAKVSSTELEETPEVPASAVPTTDQGESA
                     E"
     gene            4134..4703
                     /gene="pth"
                     /locus_tag="ACF2SP_000006"
     CDS             4134..4703
                     /gene="pth"
                     /locus_tag="ACF2SP_000006"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFD2357823.1"
                     /translation="MAIKLIVGLRNPGSAYEQTRHNAGAWLVTALAQRHNSHFKIDKK
                     MQAELTEIDINNHPCRLALPLTFMNHSGQTTRIISQFYKIEPGEILIVHDELDLPVGR
                     IKLKTGGGHGGHNGLRDITAQLGTGEFHRLRIGIGHPGHKDLVHQYVLSRPSMHDRQQ
                     IYDAIDRGIAIIPIVLSGDMARAMNQVNA"
     gene            4722..5813
                     /gene="ychF"
                     /locus_tag="ACF2SP_000007"
     CDS             4722..5813
                     /gene="ychF"
                     /locus_tag="ACF2SP_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFD2357824.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNVGIV
                     TVPDSRLDNLSNIVKPQQVVHATMQFVDIAGLVKGASSGEGLGNQFLANIRETDAIAH
                     VVRCFDNSDVVHVEGRVDPLSDIEVINTELALADMETLEKSLLKVGKNSKSGNKEAIF
                     ELKTLEKIKAHLDAGYPVRTLELTPEEEQVSKRLFLLTAKPVLYIANVDDNGYENNPL
                     LDKVQALASQENASIVALCAATEAELVELDEEDRQEFMVDLGLSEPGLNKVIRAGYEL
                     LGLQTYFTAGVKEVRAWTIRKGATAPQAAGVIHTDFEKGFIRAEVISYDDFIRFGGEQ
                     GAKEAGKLRLEGKEYIVCDGDVMHFRFNV"
     gene            6175..7290
                     /locus_tag="ACF2SP_000008"
     CDS             6175..7290
                     /locus_tag="ACF2SP_000008"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFD2357825.1"
                     /translation="MSLLKKTREQDYSILVTTQDQQRKRHLLNVISIELLILGLLLII
                     TNLCLLRAWLIISMLIVGIFILLGNLILIKKNFNLALSGHILNILVLSIIIGGNLIVG
                     KTTMSYLGWFYVSPIVAFATLGLAGLVIYGLLAIGAMFLFVFEFYIPIYSISQEYVFF
                     LDNTNHLFIFALIFTVLYHFLVQNSQYELLLKEQNYLLIADKEKFHYLSHHDSLTNLP
                     NRSYFHAHLQTLLENTDTTKEAITLYFMDLDKFKPINDEYGHEFGDQLLLLTSKRLQS
                     CFRKNDFIARLGGDEFTAIIVHKPHDQIAEALTLRIEQEFEQPFVINGQTIHCTISVG
                     IANYPRNAKNAEDLLKWADKAMYYNKRKKYQTLKQTS"
     gene            7424..8392
                     /locus_tag="ACF2SP_000009"
     CDS             7424..8392
                     /locus_tag="ACF2SP_000009"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFD2357826.1"
                     /translation="MTIDRIRELVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGG
                     KRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSI
                     WGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
                     DDYFDVIRAKTALLFAAAACIGPILTNAPIEIRDSLYNYGLHLGNAFQLIDDALDYCS
                     DAATLGKNIGDDLADGKATLPLIHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQ
                     TEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALERNY"
     gene            complement(8419..8634)
                     /locus_tag="ACF2SP_000010"
     CDS             complement(8419..8634)
                     /locus_tag="ACF2SP_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HFD2357827.1"
                     /translation="MLLQIRDFIYREGVVSTQQLSREFHLDLTALQPMLDLWVRKGAI
                     RKCQGGANCKTACFKCRTPPDYYQSVV"
     gene            complement(8615..10870)
                     /gene="feoB"
                     /locus_tag="ACF2SP_000011"
     CDS             complement(8615..10870)
                     /gene="feoB"
                     /locus_tag="ACF2SP_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFD2357828.1"
                     /translation="MTHALLIGNPNCGKTTLFNALTNANQRVGNWPGVTVEKKTGEFL
                     LGEHLIEITDLPGVYSLVANAEGISQDEQIAAQSVIDLEYDCIINVIDACHLERHLYL
                     TSQLFELGKPVVVALNMMDIAEHRGISIDTEKLESLLGCSVIPIQAHKNIGIPALQQS
                     LLHCSQKIKPLKLSLSVAAQQILNDLENQLISKGYKNSFAYYFSRRLAEGDTLIGEKA
                     FTESLLIKLQETEQNLDVLLADARYQKIHEIVTLVQKKHSDASEHFTAKLDKLVLHRF
                     LALPIFFAMMYLMFLFAINIGGAFQDFFDISTETIFVQGSGWLLQQLHAPNWVIALVA
                     NGVGKGINTTITFIPVIAAMFFFLSLLETSGYMARAAFVVDKAMRAMGLPGKSFVPMI
                     VGFGCNVPAIMAARTLDSERDRLLTVMMSPFMSCSARLAIYAVFVAAFFPSGGHNVVF
                     SLYLIGILMAVFTGYILRKTTLKGHASPLILELPAYHRPSLRRLLRETSLRLRFFVYR
                     AGKLIIPICVILGGLNAITWGGGISSGEANTDSLLSIIGQWITPLFAPMGIHQDNWPA
                     TVGLLTGMLAKEVVVGTLNSLYAQVGHVGEIAAAHFDFWGGIKAAFGSIPANLSELGS
                     ALWNPVSASAADSELSQSVYGIMSRRFDGAVGAYAYLLFVLLYIPCVSTMAVIRQEAN
                     KRFMWTSIVWSFVVAYTTSVVFYQGAKFLDHPQQSMIWILAMSLSLLFVLAVFRYSQY
                     GMGRQNAAANT"
     gene            complement(10867..11094)
                     /gene="feoA"
                     /locus_tag="ACF2SP_000012"
     CDS             complement(10867..11094)
                     /gene="feoA"
                     /locus_tag="ACF2SP_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter FeoA"
                     /protein_id="HFD2357829.1"
                     /translation="MQISELKQGDKVRLVSFGATEMQYRRRLLSLGITCGVEFSVVRM
                     APLGCPVQIEVRGTFLTLRKDEASQLVLEHL"
     gene            complement(11183..12265)
                     /gene="zapE"
                     /locus_tag="ACF2SP_000013"
     CDS             complement(11183..12265)
                     /gene="zapE"
                     /locus_tag="ACF2SP_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFD2357830.1"
                     /translation="MSLIEQYEAAIYRGEIDSDPEQREILEHMQRLAEDLQKKNDSWF
                     PWRKKHPIKGLYIYGPVGVGKTYLVDLFYQHIDEEKKARFHFHHFMQQIDAQLRRLQG
                     KKNPLQYIAKEMAKSIRLLCFDEFLVHDVAYAMILAELLQALHHYGVVLVVSSNTPPD
                     ELYLNGVHRERFIPAIEIIKKNCEVLNLNQKRDYRLGRQPLLEAYLYPLNEQTQEAME
                     KQFALITREEDCKGFITVQNRDIPYIKYGARSIWFAFDVICNFPRSQLDYLEIAERFD
                     NVFLSDVYQLTESHTAQAIMFIHFIDVMYDRGIKLIISADVPAEELYVKGEMKETFKR
                     TLSRLIEMQSVDYLSRHPRRLVKEML"
     gene            complement(12262..12924)
                     /locus_tag="ACF2SP_000014"
     CDS             complement(12262..12924)
                     /locus_tag="ACF2SP_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2357831.1"
                     /translation="MLFTDKLNTPGEHELMLEGLVGPLEAVLTVPEDVDTRYVAFLGH
                     PHSLQGGTMNNKVVTTMARAFKELHIPSVRFNFRGVGQSGGHYDNGIGESDDMRKLVR
                     QWHSEQPQAQNIFAGFSFGSYVAYRTASYCEHALLITIAPPVHHYDYKEFDPLPHPWL
                     IIQGKEDEVVPFELVSEFASQSSQVLPVIEFVETGHFFHGKLLILKEKLMDAVRDQVC
                     LS"
     gene            13161..13949
                     /gene="panB"
                     /locus_tag="ACF2SP_000015"
     CDS             13161..13949
                     /gene="panB"
                     /locus_tag="ACF2SP_000015"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFD2357832.1"
                     /translation="MKIHDFKMKKQEQKKISMLTCYDYPSACIVAESNIDCVLVGDSV
                     AMAVHGHPTTIMATIEMMELHTQAVARGLGKQFLITDLPFLGHKSSQGHTVENVKRLL
                     QAGAQAVKIEGADKDTCQTISHLVNAGIPVMGHIGLTPQSIHQLGGYKVQGKNSEQAE
                     TLLQQAATLEQAGCFAVVIECVPQDLAKTITDSLVIPTIGIGAGPGTDGQVLVWHDML
                     GLQTSFNPKFVKKYFRAKDHFIEALNSYVQQVQQMHFPANEHSF"
     gene            13961..14719
                     /gene="panC"
                     /locus_tag="ACF2SP_000016"
     CDS             13961..14719
                     /gene="panC"
                     /locus_tag="ACF2SP_000016"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFD2357833.1"
                     /translation="MQIFHNLNEWIRFRNSLSPDLSLGFAPTMGNLHAGHASLFLASS
                     KENHYTVSSLFVNPTQFNNPDDYKHYPRTVDADLELMTQNGVDFCILPNENEIYTDGY
                     AYQVQENRLGQLMEGKHRPGHFNGVLTIVMKLFNLVKPTCAYFGEKDYQQLLLIQGMV
                     KALFMDIEIKSCPTVREKSGLACSSRNNRLTPSQREIADEFAKIFHQNKSSAMISKEL
                     EALGITVEYIEEFQGRRFAAVKIGDIRLIDNYLL"
     gene            14716..15249
                     /locus_tag="ACF2SP_000017"
     CDS             14716..15249
                     /locus_tag="ACF2SP_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2357834.1"
                     /translation="MIQTNWCVITGGPSSGKTTLINHLAELGYKTAPEIARFYINQLL
                     SHDKKTLQEVKHDILWLQRKILNIMLRRERNLPKDDLIFFDRGTPDSIGYFRFHHLDE
                     AHVVRACRYLRYKKIFFCHPLPVVPDTIRDEDDVTAKKISDYIYNAYVGLNYPVIELP
                     ASSIKERLEIILSHVDT"
     gene            complement(15263..16678)
                     /locus_tag="ACF2SP_000018"
     CDS             complement(15263..16678)
                     /locus_tag="ACF2SP_000018"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFD2357835.1"
                     /translation="MFKKILVANRGEIALRIVRACKEMGIAAVTIHSEVDRFALHVKR
                     ASHSHCLSKKPLDAYLNGRRIIECAKVAGCEAIHPGYGFLSENAEFAEDCNKAGIVFI
                     GPSAPVIATMGSKIAAREAMIKAGVPTIPGSKGNLETIDDALHCAETLGYPVMLKATF
                     GGGGRGIRLCHDASQIKQQFARVQSEAEKSFGDSHIYMEKYIANPRHIEVQILADHHG
                     TVLHLFERDCSIQRRHQKLVEIAPSPQLDEATRQVLLGYAVKAAKEVGYTNAGTVEFI
                     LDKNNQPYFMEMNTRLQVEHPITEQITGIDLVQEQIRIAAGERLTISQEQVKYNGFAI
                     EFRINAEDPQNDFLPSFGRITRYYAPGGPGVRTDSAIYTGYQIPPDYDSLCAKLTVWA
                     FDWPSVLRRARRALEEMRMFGVKTTIPYYLEMIKSEEFQKGVFNTGLVEAHPEWLKYS
                     NKSLPQHKAAVIAAAIALNKT"
     gene            complement(16671..17387)
                     /locus_tag="ACF2SP_000019"
     CDS             complement(16671..17387)
                     /locus_tag="ACF2SP_000019"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFD2357836.1"
                     /translation="MYSSNYIYQHDDLELHGFLAHEEGIKQSRPAVIVAHDWSGRGEF
                     ACLKAKQLAEMGYIGFALDMYGQGQIGSNNDEKMALMQPLYSDRSLLRERLYAALEAI
                     KSISVVDNNRIAIIGFCFGGLCALDLARSGADIRGAVSFHGLLNKPENLASGRIKAKI
                     LALHGYDDPMAKPEQVHAFCQEMTEANVDWQMHMYGQVKHAFTNPQAHDEHLGLIYDE
                     VAARRSWQAMSQFLQEIFNV"
     gene            17669..18535
                     /locus_tag="ACF2SP_000020"
     CDS             17669..18535
                     /locus_tag="ACF2SP_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2357837.1"
                     /translation="MMTDSLIHIRDLLHAQFCYQMFITPIHLPIDKKYREFAKMACEF
                     IEGRRDEIIHREYPRHHVLHRFTPANKPNGKKILITHGWMSRAAYMVRLIRLLHKEGY
                     EVYAIDFPAHGEAKGIQLPWTDAIAIIKETINQFGPFYGLVGHSFGGSMILNTLNLAG
                     QLPEWQLNYKPERAILIASPTQMRTPVNKIARRFKLSGHAYLQLRQLIRQQASVDPER
                     IRLSHFISQTPDTYFLCIHGELDATINPKESINFCKYYKNARLSLLPDADHVSVLMDE
                     RVEQIALDFLNP"
     gene            complement(18670..19524)
                     /gene="rpoH"
                     /locus_tag="ACF2SP_000021"
     CDS             complement(18670..19524)
                     /gene="rpoH"
                     /locus_tag="ACF2SP_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFD2357838.1"
                     /translation="MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHS
                     EGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRL
                     VSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
                     DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDT
                     GDQGREKLLFAMEQLDERSQDILQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMK
                     KIRQYMEG"
     gene            complement(19820..20749)
                     /gene="ftsX"
                     /locus_tag="ACF2SP_000022"
     CDS             complement(19820..20749)
                     /gene="ftsX"
                     /locus_tag="ACF2SP_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFD2357839.1"
                     /translation="MLKRSQSLLAYHLQAAANSLNLFCRKPIATMMTVIVIAIALALP
                     TLFWVFTDNLSELTNRWQRGGHISLFLKPSLPEAEQTSLLEKVRTTEGVGQANIKSAA
                     DGLSELTQQEGMQDIMRYLPENPLPAVIEVVPALVIDSPAKLDLLSRKLQAFPQVALA
                     KLDMEWINRLHAILGFSGKAANALMALLALAVVFIIGNTLRLDIHNRQEEIKILKLIG
                     ATDPYIIRPFLYSGVWYGAAGALLAIFLVNIFILTLGVAVNQLANVYQMHYPLACLSL
                     RQILLLVLFAIILGWLGALLSVKRQLASIEPYK"
     gene            complement(20743..21393)
                     /gene="ftsE"
                     /locus_tag="ACF2SP_000023"
     CDS             complement(20743..21393)
                     /gene="ftsE"
                     /locus_tag="ACF2SP_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFD2357840.1"
                     /translation="MITFDQVSKRYPGGFEALSQVNFSLQKGEMVFLTGHSGAGKSTL
                     LKLIALLEWPTSGQLTVNGLKLNHLKKRDVAAHRSQLGITFQSPQLLNDRTVFDNVAL
                     PLQIQGVAYPMIAKRVHAALDMVGLLSKEKMLPVHLSVGEQQRVGIARAVVHKPALLL
                     ADEPTGNLDPKLSSEIMKIFEQFNQVGVSILIATHDLALIAGMKHRIVMLKGGRLC"
     gene            complement(21380..22447)
                     /gene="ftsY"
                     /locus_tag="ACF2SP_000024"
     CDS             complement(21380..22447)
                     /gene="ftsY"
                     /locus_tag="ACF2SP_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFD2357841.1"
                     /translation="MIKWFKRNQNSEPNLDTEEQQDHAESSMESRLEEPQELTVKEPS
                     PKESIFARFRKGLSKTRHQLGEGISRLLLGKKEISQELLEELETLLISADLGLDTTQD
                     VLKQLNEGLARKQLSNGEAVYQTLKTNLQTILSQEEKPLSIETEDGSPFVILMVGVNG
                     AGKTTTIGKLAKQFQKQGKRVMLAAGDTFRAAAVEQLHVWGERNGIPVIAQHTGADSA
                     SVIFDALQAAKARKIDVLIADTAGRLHTQSNLMEELKKVKRVMQKLDPKAPHETMLVL
                     DASIGQNALAQAKQFHEAVQLTGITMTKLDGTAKGGILFAIANDLGIPFRYLGVGEGI
                     EDLRPFDAAQFVSAIFDDDHI"
     gene            22510..23835
                     /locus_tag="ACF2SP_000025"
     CDS             22510..23835
                     /locus_tag="ACF2SP_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HFD2357842.1"
                     /translation="MHKLLISLMMLLSFQAFSQVQEYTLNNGLKVLVKEDHRAPVVVS
                     MIWYNVGSADEPVGITGVSHAIEHMMFKGTSKYPVGVFSKTIAALGGQENAFTNNDYT
                     AYYEKLDADHLATSFELEADRMNNLQLNSEEFAKEIKVIQEERRLRTDNNPQALAFER
                     FLATAHLTAPYNHPVIGWMNDLKQMKVEDLKKWYESYYAPNNATLVVVGDVNPEKVHA
                     LAERYFGSIAKRPIASRKPQQEPSALGKKMVYINAPAKLPLLLIGYTVPSVKTAKNNW
                     EPYALEIIAGILDAGESARFAKHLVRGNQVATGAEAYYNLYSRYQSQFIVYGAPSQDH
                     QIKDLEKALITELEALKKAPVSNQELQRVKNQIIAQKTFEKDSIFGQAMELGLLETIG
                     LGWKNTETYTKAINEITPEQIQQVAQRYFQENNMTVAELKPIRQEEVRP"
     gene            23832..25136
                     /locus_tag="ACF2SP_000026"
     CDS             23832..25136
                     /locus_tag="ACF2SP_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFD2357843.1"
                     /translation="MSVLRAFIISFSVIFSHNLFANSFKTEKWQTKNGVRVVFYQAME
                     VPMLDISLAFAAGSAYDGKYFGLSALTTNLINQGNSGKDATTIAEALADTGAQFNAET
                     SRDMVVLSLRTLTSKEALQQSTKTFSQIISHPDFPKEAFAREKDQLLMAVEQTEESPD
                     DVAIQNFFKTLYQEHPYAHPVHGTVESLNAIKENQVIDFYKKYFVAKNGILVMVGAID
                     SSQAHQLAEQLAQNLPAGEPAPTIPKASQLADAEKINVPFPSSQTVVRLGQIGIDHHN
                     QNYFPLMVGNYILGGGTLVSRLGTEVREKRGLTYGIDSQFVPMLGEGPFIISLSTKNS
                     EARNALHITQDTLIKFIKNGPNKEELTSAKQYLTGSFPLSLGSNTNIANLLLRMAFYH
                     LPDNYLDTYVAKINAVTDAEIRQAFQQQVNPEKLLLVTVGQS"
     gene            25133..25678
                     /gene="rsmD"
                     /locus_tag="ACF2SP_000027"
     CDS             25133..25678
                     /gene="rsmD"
                     /locus_tag="ACF2SP_000027"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFD2357844.1"
                     /translation="MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDI
                     RNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHTSLQNTIRQFDTPNLHLIKTD
                     TLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNHLLVKGGLVYIESSSPIEVKTE
                     LWKQLKLKQAGQVIYGLFEKL"
     gene            25753..26244
                     /gene="dotD"
                     /locus_tag="ACF2SP_000028"
     CDS             25753..26244
                     /gene="dotD"
                     /locus_tag="ACF2SP_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system lipoprotein DotD"
                     /protein_id="HFD2357845.1"
                     /translation="MNNNKIVIMFIFSALLAGCAGTMKFKKPPINNPSDDATIKLAEA
                     AVSVSDSMLEMAKVEKVITPPSKDNTLTIPNAYNLQARASVDWSGPIEELTARIAKAA
                     HFRFRVLGKSPSVPVLISISTKDESLAEILRDIDYQAGIKASIHVYPNSQVVELRYAK
                     IYS"
     gene            26225..27136
                     /gene="dotC"
                     /locus_tag="ACF2SP_000029"
     CDS             26225..27136
                     /gene="dotC"
                     /locus_tag="ACF2SP_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotC"
                     /protein_id="HFD2357846.1"
                     /translation="MRKFILSLSILLSALLVACSSRNHYGDTGSLAGLQAMADSKYTR
                     AQKKQKMGKIREMALKETALSVGAQAGLAWRAKIIDEQLNKQARNLDAIYDFNSLVLE
                     HNILPPVLLEGRNTLNLADAQSIRVSDRTYKVAKQAHFITTPPTWRQYLWMDYVKPEA
                     PNVTLLPKTKAEKEIWCIYTERGWKNGIDQANTILEENIARIKEDFGGMILYRKLLAM
                     NMVSPPYVSHTDLGVTGDGSEIHIDDRVLRITALPELNVNSAEWRAAVAKDENALERF
                     KNMEKLANQAKIVITNKSWQPIIAPVS"
     gene            27136..28269
                     /gene="dotB"
                     /locus_tag="ACF2SP_000030"
     CDS             27136..28269
                     /gene="dotB"
                     /locus_tag="ACF2SP_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system ATPase DotB"
                     /protein_id="HFD2357847.1"
                     /translation="MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPI
                     FAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYR
                     YRVNATACLVEGHDAIQITLRTIPTTPPKLSTMSLPDNIIEAIAPQEGIVFITGATGS
                     GKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFAD
                     GVRNALRRKPRLIMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFS
                     GEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTS
                     ATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ"
     gene            28351..30078
                     /locus_tag="ACF2SP_000031"
     CDS             28351..30078
                     /locus_tag="ACF2SP_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFD2357848.1"
                     /translation="MIFHRKCLSHFFNLFISLILGSSLNWASVNQHSTDSPTLNIKIL
                     GINDFHGQISSGRTVKNEPVGGAAILAAYIKEAQLGMEDRSIITIMGDLVGASPPSSG
                     LLNDEPSILFINSLGNNQCKPESRMNPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPP
                     TDSWISLPSYPGSSYPYVSANIVDAKSEQPLFPPYIIKRIHNIPIAFIGAVLKDAADS
                     MFPANAEGIKFLDEADSINRYIPEIKAKGVNVIIVLIHEGGNQIPYEGDTQTASAVEG
                     HIKEIVYSLGDEIDVVMGGHTHQFLNAFLRNRNGHEILVTQANSYSAAFAEVTLQIDT
                     RNHAVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAEYDLLRK
                     QNEDGESSLGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNRIV
                     IITLTGNDIYDLLEQQWMGSYINMLQISGFAYTYDSHQPLGHKIIEIRHQNKPLLREK
                     MYTIATSDFLASGNGVFSVMKRGKIIHIGQSDHDTVIAYIKSLPQPFHASIEARIKES
                     ETGLKTGVG"
     gene            complement(30120..30917)
                     /locus_tag="ACF2SP_000032"
     CDS             complement(30120..30917)
                     /locus_tag="ACF2SP_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2357849.1"
                     /translation="MPSTYILNTNDKARERLSLQHQLYAKSSLDLLSEVGPSAAKTAL
                     EIGCGSGDMTLELAKLIGREGSLLTIDLSQDQLDHTQQRTRTYSNIRFKVWDVNHLSD
                     LGEKFDLIYCRMVLHHVADARSVILQMKNCLNSGGFVVCEEPSLFDSTFCSPPSPSYD
                     QFTQWARSCFSKNNRDFQIAHRLEQEFSSCGFEIEHCSLFQPLLRTNKEKLIYSMALD
                     DLTPQLLELGIAKREEIKQLHNELEDLAKTNSTMCWIRMHKIIAKLN"
     gene            complement(30947..31891)
                     /locus_tag="ACF2SP_000033"
     CDS             complement(30947..31891)
                     /locus_tag="ACF2SP_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2357850.1"
                     /translation="MKPFVYLTNQFLEPIIPMLIPDWNIIHGWKMASGVDQSRVVALA
                     TTVWDQIDHSFLMQFPNLKIISHLGIGTDNIDINFLKQNHIILHSQPNAGVHDTAELA
                     IALLLALSRRVILNDRYTRNNEWVEKKPRFLGNHLLGKQLGLVGFGQIGEKIAQFAEP
                     FGLKIAYTARSQKNSPYLYCPTAANLASISDFLIICCSGGIDTQHLINKQVLDNLGTE
                     GYLINVARGSIVDQNALIDALQRHTIAGAALDVYQYEPEVPLALRQLDNVVLSPHMGS
                     STKENLNQMFQLQAKHLNQYLNEPLWQRESHLTLFEEQ"
     gene            32128..33150
                     /locus_tag="ACF2SP_000034"
     CDS             32128..33150
                     /locus_tag="ACF2SP_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HFD2357851.1"
                     /translation="MPVIPVEEFDGFILKLGKNNYHFCKNETPFNNSCSANIAIDKYC
                     TSKLLEKAGIPVPKAISLNCSEFQRNLHKDKIAQLNFPLVIKPIDGSLGIGVLCNIKT
                     WEELESHLTKYFSSYQCLIIEEFHGKLNSYRVLVFNNRILGIVQRYPAAVTGDRVHPI
                     HELIKQENLNRKRINEALGEITLDEEARIKLQELGITENYIPSFGEQIVLCYTSNATR
                     GGSYVTINKKMCKQNRKLLLQAAKILDLQLVGIDVECSDIINVPIEQSKGVILEVNHK
                     PSIRIHEFPMKGTPQKVTKKIMRSFITRHPLSYLYSIYNNQPTAFYVRSSILIIIMGL
                     ILLAVW"
     gene            33424..34311
                     /locus_tag="ACF2SP_000035"
     CDS             33424..34311
                     /locus_tag="ACF2SP_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFD2357852.1"
                     /translation="MNPVSILDASLRDGGHRTNFHFKDDDLKQILLPLDNSGIEYIEI
                     GYRNGSLHPIENIGKAGMCPQDYLLYCQSLIKKAKIAVMVHPHNVNEQDLLELKNCGV
                     KLIRICIAKGDLVKAVPLIKLAKNLNFETSVNIIHISYYTESELDSLIEEVSKHEPDM
                     IYFADSNGSLFPDRINCLYKKYTRQYKVSFGFHAHDNLGLAQANALAAINAGAQFIDA
                     SLAGMGKGTGNLKTEFFIAYLHANNIKKYNLENVLTAANYVRDALKIGQEPIEMSEFI
                     RGISDLSTADLKSYKPGKL"
     gene            34450..35157
                     /locus_tag="ACF2SP_000036"
     CDS             34450..35157
                     /locus_tag="ACF2SP_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357853.1"
                     /translation="MAKKRLLIIGPVSTQSSSLRHLSQNLSFLNPYYSIECLDPLVTL
                     QECPDEEYYGIWQNQLKPLIKIYDAFVGFSFGGVILQQCFSILSEEKKQIILFSTPTF
                     ADSILREKLETVISLCSQNKLEEGLSSLYQAVYAPHSIPDASLQYLNRELAIKRLIFG
                     LTKVLKTNCVSILNHCNVNHLHLIGEKSQLVNTNNVIKPSCGQLIVVPEASMRVLESN
                     PTFCKEAILKALNHEIH"
     gene            35144..36991
                     /locus_tag="ACF2SP_000037"
     CDS             35144..36991
                     /locus_tag="ACF2SP_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HFD2357854.1"
                     /translation="MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPAILSYGDIG
                     WEFWKKEGTAIPSRTWQLIASSDTVLLGAITSKPQREAKQELSKALRKSNPYYVSPVI
                     QLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHNLLAESQ
                     HWQTIPADEASCALRLQSKPGLTRLFDFAFNHAMQTGMPRVTLADKPNVLRESGEFAR
                     KIFESTAQKYPKIQADILNVDAVALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGL
                     GLAPSANIGDKGSYFEPVHGSGPRMKKNCANPSAMFLTISMLLNHFGYPDRAKKIVNA
                     VMQVIKEKRYITYDLGGHSTTTDMANAVIERCARLEASCLSTDFNPVPKENIIESDAM
                     GNLLQQLMHCNSAEISDALDSCGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKEAV
                     FHNAANYIDKVPKHSVIVIDNNGQTDCTVWGDLLTHTALRNNIMGTVVHGAVRDVESI
                     RSTNYPVFCTGIYMCSGKNRVYKANEQCPLFINGVTINPGDIVFADDNGVLVIPNVLL
                     HEVVNKAIAIRLTEERIKAAVASGSTLEQAREDYYYEQPWLGINKKREQ"
     gene            36991..37851
                     /locus_tag="ACF2SP_000038"
     CDS             36991..37851
                     /locus_tag="ACF2SP_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HFD2357855.1"
                     /translation="MFLPEKLKFIDIHYHAAPDLYIRRRNAIEAGRLYHSLRGAVVLK
                     SHIGSTGIQATIAQEMGLPVFPSVVLNHINGGINYRVVIRALSEYQPLFSGKMIVDFP
                     TITGRKLQSKLSRDLTHNTLKQYTGQKETLFGSKQQLRKEVIDILKMARDYPIVLTSG
                     LASREEVYRLIDACNQYNVPSLLLSQPSNPLTGLKFTELNELIKNEWLWVEQTALTFI
                     LEHQDKQDFSQVLTHIPRVIYSSDLGQTSQMDIPEWLHFSKRIFHELDLSEKRKEELI
                     LSNAIALLNL"
     gene            complement(38112..38828)
                     /locus_tag="ACF2SP_000039"
     CDS             complement(38112..38828)
                     /locus_tag="ACF2SP_000039"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFD2357856.1"
                     /translation="MLVTKEVEYEDGDTVCKGFIAYHASISKPLPCVMVAHDWGGRGE
                     GVCNKAIQLAGMGYIGFAIDMYGNAKLGKDKVEKRALMTPFRENRGKLITRINAAFSK
                     VVQLPEVDRDNVAAIGYCFGGLCVLDLIRSGAPLRGVVSFHGVLLPLEGESNKPLHAK
                     VLILHGYDDPLVPIEQVNQFSIEMTERKVDWQVHVYGQTAHSFTDPNANDDEMGLHYN
                     KLADQRSWQSTQLFLQDLFA"
     gene            complement(38959..42066)
                     /gene="dotA"
                     /locus_tag="ACF2SP_000040"
     CDS             complement(38959..42066)
                     /gene="dotA"
                     /locus_tag="ACF2SP_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotA"
                     /protein_id="HFD2357857.1"
                     /translation="MNKLAITVLLCFFPALALAESGALSFAPPASDLSVVFLGNLFGV
                     VDGVLHGTGSQIMGNMFGVFNSAVLALGGIIIMYTLMVSTMNTAHEGQMLGQKWSSIW
                     IPLRSTFGLALLIPKASGYCMMQVFFMWVIVQGVGAADKIWEAALSYLNRGGVIIQAQ
                     SDPTKSLTDKGGKSTGIAKGAFTILSGQVCMLGLQKQLQTQRNLYLSQSNSPPCGGNP
                     TPEMNTFCRTAIPDFISTVNFVKKQSDDKSKGPFSLDMPNFDKSSPFYFLNGICGTVT
                     WNSISDLKADMGKNIKLTSSQLETAQLSRAIAIQQMYVTLSTVAQVMVNNDPAFSSTS
                     STGNSKNDFSAIAKQQFGVPYKASGQVCTEYQQVCQTWGSIPSSTGSTPGVLFNGTEF
                     LGAINDYNGIMTPTINLIKQSKSAEYDDKSRDFIQEANTKGWIMAGSYFFDLVKLNGS
                     AVKDESDFDSDTGLDNSNFDPAQLTKPFGKTCQGTYALLCNWFQNKSDKLVQVQSLIN
                     GAPALSQDGVKQPDLSPDPKRAYVEGPLSSTVYGFINNSMMVQLPGQPGIKPLTFANL
                     INFKVDTSLYYMKHQDFDCGRVKILFFSFCLGRMMGDLFYNYVFRYVYNFFLAIFGEM
                     INSIVMAFLMIPLQGMKDIFIVGVQTLAQPGVNPIVALANMGTMYINFSGTLWLTLLN
                     MAVVSSLIPLFGIFIFALIMMAMPLLMAWIGTMVSIGFVTAYYIPVLPYMIFTFGSFA
                     WLIAVIEAMVAAPIVALGVTHPEGNEAFGKGEFAIMILVNVFLRPSLMIIGYITAIAL
                     SYVGVWILNAGFDHAISYIQSDQGIETWKKGDFADKNANSAYCQMYPADCKMIQAGQT
                     VANWTGSKIDTSQWDDFGSKLSGDYQSTALQGGYSGWAGVYAFFFSILIYTTMYLIIV
                     QKAFTLIAHLPDKVLRWIGGNPESFGQETMQWGEEAKGKVEKAGEATYGAEKAIGDKL
                     GAKGQEMLGKFGPQAKEIAGNAGDVSGKGKNSGSQTGPSSKPDTGQQLGGSDSGAPTS
                     TPPPE"
     gene            complement(42063..42518)
                     /gene="icmV"
                     /locus_tag="ACF2SP_000041"
     CDS             complement(42063..42518)
                     /gene="icmV"
                     /locus_tag="ACF2SP_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmV"
                     /protein_id="HFD2357858.1"
                     /translation="MKKKSGSRIVRVITRIINVRKWVDWDRMKSFTLYLVNGVKRLFV
                     PQEPTHVESFDEAVKKLKLNEADLIIKQKALFRLSVIMVIAALMLLIYTGYQLFYGSW
                     KATIISLVVVMIALVLAFRYHFWYYQIKQRKLGCTVKEWYRQGLLGEKE"
     gene            42631..43086
                     /gene="icmW"
                     /locus_tag="ACF2SP_000042"
     CDS             42631..43086
                     /gene="icmW"
                     /locus_tag="ACF2SP_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmW"
                     /protein_id="HFD2357859.1"
                     /translation="MPDLSHEASAKYWFEYLDPMIYRVITFMESVENWTLDGNPELEE
                     AMKQLGQELDDIEKIDLGLLAEEDKFIRIVGNIKSGRGLRLLQAIDTVHPGSASRVLI
                     HAEETSLSSNDPAGFFLKRNIVFERLRLLSRVFCQYRLKLVLRALEGDE"
     gene            43086..44504
                     /gene="icmX"
                     /locus_tag="ACF2SP_000043"
     CDS             43086..44504
                     /gene="icmX"
                     /locus_tag="ACF2SP_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmX"
                     /protein_id="HFD2357860.1"
                     /translation="MKVLPKLALANLFCFTTLSVSVCAQSGIDTSGQQTSTNTSNLVT
                     YLTNLGKYLGYDITQSNKTPNPPYSQLFNSNVAQLIQNYAYNTFLGAIPVNAMSQSFM
                     NFVTQNVQGSSLINNLANNTFKYQNFSTPSSGLNGTVSANSLIDQSTANAQAPTPSGV
                     FSDPQTTPSGTYLKDPVSQAVFNILGTPDYSFCMDNEQKNWLPNCNLMYQNLVMQNVI
                     GTLPPAQTSGSTPAFYTYNYNQSLIGQLNGNSLIAPLLLDTNPAPLQQLSSSGGLKAI
                     SQAQQALNFIRYASAQVTPPSLPKLSAYSELWNQATAKPNSDGYNEVQQKQAAATLSG
                     YFNNLRVYAAQTSVGVSNLYYILSKRLPQNMSGDQSNQNLTSQALNEFNMATRRLFDP
                     TASNTPGQPNQQWIKQINDASPATVQKEIAVLLAEINYQMYLDRQIQERILLTNSIML
                     LQNLKAAQPTADFSSQGSPSEQ"
     gene            complement(44511..46151)
                     /locus_tag="ACF2SP_000044"
     CDS             complement(44511..46151)
                     /locus_tag="ACF2SP_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HFD2357861.1"
                     /translation="MSSLLSRRKTILVLLIAIYCAYDLMVALYTAWDFTTDDAYISWY
                     YARQLVSGKGLFWHVTLPPVEGYSNFLWLMIAALVIKLQLPLVSSMKVISCCSLGLAL
                     LFLYRLARLFCSPLLSILPVFLFSHYTGVVWWAVSGLETLFYCALSLALIWQCTLAFG
                     YQTVGDRPWPNRFSLYSTTHWIITNMLLLLLSLTRFEGIVWIIPLVVFIGCQYLRAQR
                     GLVFKDHKRLFLWVSTTFICFVLPYLVYFIWRLIYFGHWIPNSYRCKALAYGQFLAVD
                     FDYFLFITPLLVLSVPYLLSANKDCRHVLLWLPSVLYTLLLWKANPVVTYGLRLFLSP
                     FALFSILPVLGLNQLIQYFKFFRWDPEYLATVLMLFITWFFVPGNDLNHLSKSVAEYN
                     ERTQNRMTIADLLNRKAKRGDSVLLDDCGIIPFFSREDIRFIDAQCLNNNDLTKKPFK
                     NDLSLYADYLNSKVKPDWVITNYYPMESRGDYLTDLLYEKHFFKNYQLVSTLNSGFYL
                     NKNQPQGKKMIDFVYQIFKRVNKPGYNGSLELESQEPK"
     gene            46518..49061
                     /locus_tag="ACF2SP_000045"
     CDS             46518..49061
                     /locus_tag="ACF2SP_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD2357862.1"
                     /translation="MSKPYYFYVSGMNCTSCSNAIESFLRNSNLIKINTFHADMSTPD
                     PKKIVISLSEEKENEHESNWKTIKKLIDDTGFTCENYEFPQVRDQKPALPLEKTQTTV
                     QQEASQLSALFSSAKKIITSHWLLGGLGCITGVSLMILTMALGGLPLAVMIPLASIST
                     LLTIALGAQSYYEAWKKLVQSRTLTMDTLFSISTISVLVVSLASFFVPWLPMMFEAGL
                     LIYGFRHIGIAIEDTIKDKISSAKFQDRAPQKVRLYRHPDFKKTRIDEIQPNDIIQVK
                     PGELIPLDSVCEQDTLIYPTIITGAVIPKQFRSGEKILAGMRLANDAKPLSLRVLKTA
                     KESYLARLDESIAQSVLEKAPIEVKTGQLLSYFIPSIIALSILSGIIVGLIATPALAI
                     QCAISVLVSACPCTLGLIIPLAVKTGIHKGAEHGVQFKNSRVLQLAEQIDVVIFDLNG
                     TLTTGVPVVKEYAVLNDADLSSEQFLSICNVLEEQSKHPFGKAIQSFTQGINKQKFNI
                     ANLDHSNHSGVSAEINGIQYTIGSKTLMQEIGISTFSLENQMNLVAGDSLVFLARNKQ
                     LLGYLVITDPLRKDAKQTVDALIKMGKEVHLCTGADEKTAYRYAQALGIKKVHANCVA
                     TTINTEDRSKPVYIKSLQNQGLKVAMIGDAANDANALAASDFGIAVLSQDSDEITQQH
                     ASAIIQSGTILPIANAFAISKQTVNNINQNLLISLGYNLTTVLIAGGLLVALGFVLNP
                     AVGVALMVTQACFVLLNVYRFKEQPLQHLQDKVQEKPEPAPSSYEAMKKHSPNFHTAP
                     EINHSQAIQSGKQPTNKNKSPSFWSDYFPELFEEEKDTSTEHLMNNKIG"
     gene            49312..49842
                     /locus_tag="ACF2SP_000046"
     CDS             49312..49842
                     /locus_tag="ACF2SP_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2692 family Dot/Icm T4SS effector"
                     /protein_id="HFD2357863.1"
                     /translation="MPTTFEDKSSKVPSLKSLAANAVQKTNANLFFRLHSLETPPEIK
                     KGFIDKELEALTHELTEDYQTKVEAKNEKIEECSSNLSSNECFVKCSAFTLTTLMAGV
                     HVGIYYILKAAAVDPSTQIAYISSIPATICFSMCVGVCLNRQITKCLSSCFTPSVPDK
                     ITVDLDELGRQSHVSP"
     gene            complement(49914..50714)
                     /locus_tag="ACF2SP_000047"
     CDS             complement(49914..50714)
                     /locus_tag="ACF2SP_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFD2357864.1"
                     /translation="MDNESLLAILNNWSATNYAKGNYILREISSKFLAKISIHSNDYL
                     LDAGCGDGSFTQMLANLVPDGYVLGLDRSKTMIDYANKHCRSINVRFDIGDIQEPIIY
                     GPFDNILSFWCLHWTDLEKSLSNLYHVLKPGGKICAIFSSGKQSTLFEVLNSLQFQNL
                     YPDVAKNVVQSKMMRENYSIQCRRALDNLPFKSISFVVEHCQVELPDLGLFENFIHGA
                     PLFKDLPGKMYQDIVNSMVQFFDEYCKKKYGGKWVYETTPYFLTITRE"
     gene            complement(50838..51695)
                     /gene="legD1"
                     /locus_tag="ACF2SP_000048"
     CDS             complement(50838..51695)
                     /gene="legD1"
                     /locus_tag="ACF2SP_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegD1"
                     /protein_id="HFD2357865.1"
                     /translation="MILIFEEHRMQLSSEQCDFFFTNGYLVINNFFSESVCDLLKQRI
                     ETLLENNQAEIPKTIFSTQTNEHAKKQYFLDSGDKIHYFFEPGAFDEAGDCLRPFNQS
                     INKIGHALHELDPVFRQYSRDDRIKKIAHQLGLNTLGLVQSMYIFKQPGIGAEVLCHQ
                     DSTYIFGEDSDALGFWFAIEDATLENGCLEVIPSPCTTPLKQRMFRRENEIYFENFDS
                     SPWPEENSVPLPVKKGALIILHGRVPHKSQANFSNQSRHAYTLHLVDISLPYPEKNWL
                     QWPNGIPCL"
     gene            complement(51783..52769)
                     /locus_tag="ACF2SP_000049"
     CDS             complement(51783..52769)
                     /locus_tag="ACF2SP_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFD2357866.1"
                     /translation="MLVPLIRTLQNKLPNAQLTWIISKPAFYLVEGMEGVEFIVIDKP
                     KTLADYWRFRKQMLPRQFDVLLAPQASFRTNWLIPFIKAKRKVGYDSHRANDGHRLFI
                     KESITPGLEHTLEGFLKFAEPLGVTEKIIHWDLPITKADYEWAQNHLPEQERPILVVN
                     PAASKPERSWLAERYIEVLKQAQVKWDVKVVLTGGPGDHDKSLAEEIAKEIPVVNLVG
                     KTKPKQLLAVISKAKAVLCPDTGPSHMAAAVGTPVVALHAVTNPSISGPYTFQHLVVN
                     RYSQAIQAVLGVSPEKSVWGTQVHGYEAMKLITVDDVMEKLTEIFSGLMNNT"
     gene            complement(52813..53007)
                     /locus_tag="ACF2SP_000050"
     CDS             complement(52813..53007)
                     /locus_tag="ACF2SP_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFD2357867.1"
                     /translation="MRKTTKEPASAKKNYVVHRHQLPLSCPTDEMELWNAHPKVYLPI
                     EKTGVEVCPYCGSRFVLQND"
     gene            complement(53030..53971)
                     /gene="miaA"
                     /locus_tag="ACF2SP_000051"
     CDS             complement(53030..53971)
                     /gene="miaA"
                     /locus_tag="ACF2SP_000051"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFD2357868.1"
                     /translation="MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMN
                     IGTAKPSIHELQRAPHYLIDIKDPVESYSAAQFCTDALSLCAEIIKRGNIPLLVGGTM
                     MYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
                     IQRALEVYYLTGSTLSTYLTGPHVQPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEG
                     FIEEVQQLQAKWPIQINLPSMRCVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQL
                     TWLRHWEGALFYDSQNVGFNIDIIAKIREILDNTVSN"
     gene            complement(53964..55694)
                     /gene="mutL"
                     /locus_tag="ACF2SP_000052"
     CDS             complement(53964..55694)
                     /gene="mutL"
                     /locus_tag="ACF2SP_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFD2357869.1"
                     /translation="MVLRIHQLSPAIANQIAAGEVIERPASVVKELLENSLDAGASVI
                     GVDVNYGGLLQITVSDNGSGISGDDLPLAVAAHATSKIRTLDDLYSIDSMGFRGEALA
                     SIASVAKVTIISKPEEQDNAMMLRVEGENRTLSPCARNIGTTIDVSDLFYNAPVRKRF
                     LKNEKLEFQAIEMVVKRFALSAPRIALTLKHNKKLIFSLPPALSEQAKAIRMGRILGN
                     TFMREAIFLDVEHSGMSLYGWISNHRLQRSQNDRQWIYVNQRMVKDKLLQHAVKQAYD
                     GLLHAGRFPICLLYFTLPASEVDVNVHPTKHEVRFQQPRLVHDFFTSQLTKALQSTLD
                     HSDTREYAYAASSCNEQQNTVSEPSHICNDRESHEGARALSPSWSANLLSSSITNKGY
                     KKTSSVYHPETESQWVILNERFILVFIKQQPYLADIVTLFQEWIENQLMQKALPLESR
                     PLLISIRYPLPNQWIHKQEQLKHELARVGIQIEYPNADDLLIRSIPLNAPYLDVRYFF
                     NVLCELNIFHTEQLIALISRSQMFDPKQLTAEERIEMNQLLLELSLKEEKHNICKALT
                     IDDCRMLLHE"
     gene            complement(55685..57115)
                     /locus_tag="ACF2SP_000053"
     CDS             complement(55685..57115)
                     /locus_tag="ACF2SP_000053"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFD2357870.1"
                     /translation="MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQ
                     GSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDV
                     KQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISS
                     QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAI
                     TLKLKRLIDRQPGMRAVLTRSGDYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNG
                     ASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVRSVLIDLSQTATINA
                     GLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNP
                     AYQERLSQAIFQGLKNYFWDHPPHGTRIEAMLSPNFHIVKKGETLPAIAARYRVSVSA
                     LQSINRLQARARLMPGQKLVIPGTWS"
     gene            complement(57112..57594)
                     /gene="tsaE"
                     /locus_tag="ACF2SP_000054"
     CDS             complement(57112..57594)
                     /gene="tsaE"
                     /locus_tag="ACF2SP_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFD2357871.1"
                     /translation="MAIEDNDNFITLDLVSEQESKRFAEKLAFCISAPLVITLSGDIG
                     AGKTTIIRAMLKSLGVISAIKSPTFSLVESYNCGQFHIHHFDLYRIHQEEELEYLGFR
                     DYFSNQSVCCIEWPEHGGKTLPPVDIQFNLIIKGTGRLIQIAALSVAGKRILACLAGE
                     "
     gene            complement(57720..59201)
                     /locus_tag="ACF2SP_000055"
     CDS             complement(57720..59201)
                     /locus_tag="ACF2SP_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFD2357872.1"
                     /translation="MNKLENALYRCDQIKACEQQAATLYQKDENALMALAGAEAFSFL
                     QKIYPHVRHIAVFCGAGNNAGDGYVLARLAHEQGFSVTVYQCKSIEDLPSAARNAARL
                     ALTSGVHCQSADEPLDGDAELIVDALLGIGLQGAVHGVIANAIHQINASGLPVLSIDI
                     PSGLNADTGLVENICVNATATITFIGLKTGMYTLDGPDYCGVIHCAHLNLEACRTKLT
                     RFASLLRDSTLPLPLAARKKNSHKGDYGHVLVIGGGPGMPGAVSLAAKAALKTGAGVV
                     TVATWPDYAKSALPLIPEAMIWGIKTAGELMPLLAKATVCVIGPGLGDSDWAKTLFLT
                     AITTQLPMIIDASALRLLSQHPQMDDNWILTPHPGEAASLLSCTARDVQMDRYHAASA
                     IQKQYGGVVVLKGVGTVIQTAERNIFVCPQGNPGMATAGMGDILSGIIAAFCAQGFTL
                     SDAAKLGVWVHAVAGDKVAEKSGEVGVLASDLLDEIPSILNCI"
     gene            complement(59329..59724)
                     /locus_tag="ACF2SP_000056"
     CDS             complement(59329..59724)
                     /locus_tag="ACF2SP_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFD2357873.1"
                     /translation="MAMTSNKPYLIRAFYDWIVDNDLTPYILVDASNPNVQVPREHVQ
                     HDRIVLNISPSATRGLLLENDRIVFTARFSGQTEQIFVAPNAVLEIYAKENGRGIAFS
                     VDEDDEPPPKTSSPSESATKNKPSLKLVK"
     gene            complement(59727..60347)
                     /gene="sspA"
                     /locus_tag="ACF2SP_000057"
     CDS             complement(59727..60347)
                     /gene="sspA"
                     /locus_tag="ACF2SP_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFD2357874.1"
                     /translation="MAIVAKRTIMSLFSDVDDVYSHQVRIVLAEKGVNVEILAVKQDD
                     PGADLLSINPYGTVPTLIDRELVLYEARIIMEYLDERFPHPPLLPVYPVARAEARKMM
                     HRIEQDWYYLMNAILREESVEQARANLLESLTSLDPVFADKPYFLSDEFSLLDCALAP
                     LLWRLPKLGLEIAPNFKGIISYMQRVFKRDSFQTSLTDAERQIRAA"
     gene            complement(60595..61341)
                     /locus_tag="ACF2SP_000058"
     CDS             complement(60595..61341)
                     /locus_tag="ACF2SP_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFD2357875.1"
                     /translation="MMKIKVLLVAALAFLISNAAFTENLAMDYVPVDINDKESLQRGA
                     KMFMNYCSGCHSLKYMRYNRMAQDLGLTTFDGQIAEDLLKNNLIFTQANIYDPIQIAM
                     SPEDAKQWFGMVPPDLSLSARDRGPVWIYNYLKSFYSDPSRPFGANNLLMPDVAMPNV
                     LEPLIGKVILVKDKNSHANSLLVVKRGEMFEGQFDSALRDLVNFLVYVGEPAKLIRYK
                     LGVFVLLFFCIFLIAAYSLKKAYWKQLKLE"
     gene            complement(61338..62552)
                     /locus_tag="ACF2SP_000059"
     CDS             complement(61338..62552)
                     /locus_tag="ACF2SP_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFD2357876.1"
                     /translation="MNGLVNWLDERFPLVSTWKAHFSNYYAPKNFNFLYFFGSLALIV
                     LVNQIITGLWLTMFYTPNAEQAFSSVEFIMRDINFGWLLRYMHSTGASAFFIVIYLHM
                     FRGLLYGSYQKPRELVWLLGMFLYLLLMAEAFFGYLLPWGQMSYWGAEVITSLFGAIP
                     YIGKGLAVWLRGDYSVANPTLQRFFALHVIGIPILMIILVFLHIVALHKVGSNNPEGI
                     DIKKALDKDGKPLDGIPFHPYYTVKDFVGIIVFSILFFAVVFFAPEMGGYFLEHANFV
                     PANPMVTPDHIAPVWYLAPFYAILRAIPDKLIGVVCMAAAILLLFFLPWLDKSNVRSV
                     RYKGMYSRISITLFAFSFLLLGYLGTTPVTPVRLLLARIAAVIYFSYFILMPIYSRFE
                     KTRPVPTRIGEK"
     gene            complement(62563..63180)
                     /gene="petA"
                     /locus_tag="ACF2SP_000060"
     CDS             complement(62563..63180)
                     /gene="petA"
                     /locus_tag="ACF2SP_000060"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFD2357877.1"
                     /translation="MTDLNQDVSNHNQDEQLDEERRRFLLHTTCVLGGVGAACALTPF
                     ITSWLPSSKAQAAGAPVKVDVSRMEPGEQAIIEWRGKPVWIIKRSKDMLNQLQKEDES
                     QLRDPDSLVDQQPEYAQNKFRSINPEFLVLIGICTHLGCSPKYKPNLGELGPDWPGGF
                     FCPCHGSMFDLSGRVFKGVPAPINLEVPPYHFLDDHTIVIGEDAK"
     gene            complement(63354..63785)
                     /gene="rpsI"
                     /locus_tag="ACF2SP_000061"
     CDS             complement(63354..63785)
                     /gene="rpsI"
                     /locus_tag="ACF2SP_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFD2357878.1"
                     /translation="MAEMKQYYGTGRRKSSIARVFLRPGKGDIKVNDRTLQEYFCRET
                     SCMIVMQPLETVDLLNKFDVYITVTGGGISGQAGAVRLGIARALVAYDEDGLAEGAEP
                     SPDSFRRKLRARGLLTRDSRRVERKKVGLHKARRATQYSKR"
     gene            complement(63791..64225)
                     /gene="rplM"
                     /locus_tag="ACF2SP_000062"
     CDS             complement(63791..64225)
                     /gene="rplM"
                     /locus_tag="ACF2SP_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFD2357879.1"
                     /translation="MKTFSAKGNEVKRDWFVVDASEKVLGRLATEIARRLRGKHKAEY
                     TPHVDTGDYIIVTNAEKVVVTGRKFKNKMYHHHTGFPGGIKSASFEKLQDKNPTKIIE
                     LAVKGMLPKNPLGREMYRKLKVYAGSEHPHTAQQPKQLEIEE"
     gene            complement(64565..64882)
                     /locus_tag="ACF2SP_000063"
     CDS             complement(64565..64882)
                     /locus_tag="ACF2SP_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HFD2357880.1"
                     /translation="MLSHYTKHVFICTNQKAPGKQCCANSGGEEFFDFMKSKLLEFDL
                     HGPGKVRVSKSGCLGRCSSGPCIVIYPEGVWYTYSSFEDIEQIIKYHLIDGEIVEPLL
                     LDR"
     gene            complement(64884..65177)
                     /locus_tag="ACF2SP_000064"
     CDS             complement(64884..65177)
                     /locus_tag="ACF2SP_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFD2357881.1"
                     /translation="MNALSKAIMAETLCDELKLNKPVAKEMVENFFEELRHALENGQH
                     VKLSGFGNFTLRDKPQRPGRNPKTGEEIPVEARRVVTFKPGLKLKTKIEKIGK"
     gene            complement(65180..67561)
                     /gene="pheT"
                     /locus_tag="ACF2SP_000065"
     CDS             complement(65180..67561)
                     /gene="pheT"
                     /locus_tag="ACF2SP_000065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFD2357882.1"
                     /translation="MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTH
                     VIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGG
                     LQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
                     DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVAPAIDDGLRVNLIHSEACPRYCG
                     RIIRNLNLEAKTPLWMAERLRRGGIRTLHPVVDVMNYVMLELGQPMHAFDLAKINGEI
                     NVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGANSAVQELTQHVFLE
                     SAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVI
                     ESFDKKFLPGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVITKETNHFFEVTIP
                     SHRVDLQQDADLVEEIIRLYGYDKLQAQPMQTSVQAGLISAKEKIATHVSSWFSAKGY
                     HETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYNSHRQQT
                     AVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFA
                     SLKLNDVEFIQSSHHALHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELN
                     LESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQIERVIRNTVKEDWLKSFDVFD
                     VYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE"
     gene            complement(67819..68832)
                     /gene="pheS"
                     /locus_tag="ACF2SP_000066"
     CDS             complement(67819..68832)
                     /gene="pheS"
                     /locus_tag="ACF2SP_000066"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFD2357883.1"
                     /translation="MVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKG
                     LANLSAEEKPKVGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNHS
                     TGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHDTFYF
                     GDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQA
                     TLAGLKGLLQDFFAYFFGRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTG
                     WLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTFL
                     RQF"
     gene            complement(68972..69331)
                     /gene="rplT"
                     /locus_tag="ACF2SP_000067"
     CDS             complement(68972..69331)
                     /gene="rplT"
                     /locus_tag="ACF2SP_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFD2357884.1"
                     /translation="MPRVKRGVTAKARHKKILDQAKGYYGARSRTYRVAKQAVIKAGQ
                     YAYRDRRQKKRQFRALWITRINAQARECGLSYSRLIDGLKKASIELDRKILADMAVHD
                     KVAFAAIAEQAKAALAG"
     gene            complement(69347..69547)
                     /gene="rpmI"
                     /locus_tag="ACF2SP_000068"
     CDS             complement(69347..69547)
                     /gene="rpmI"
                     /locus_tag="ACF2SP_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFD2357885.1"
                     /translation="MPKLKSHRGAAKRFRKTASGAIKRRGAYRNHILTKKSTKQKRHL
                     RVEAGTLKPCDARLAERMLHGS"
     gene            complement(69568..70104)
                     /gene="infC"
                     /locus_tag="ACF2SP_000069"
     CDS             complement(69568..70104)
                     /gene="infC"
                     /locus_tag="ACF2SP_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFD2357886.1"
                     /translation="MSAPTKRENDRARINEQITVPEVRLIDVDGNQAGIVSTREALRA
                     AEESGLDLVEISPTAKPPVCRIMDYGKFLFELSKKQAEARKKQKQIQVKELKFRPTTE
                     DGDYQVKLRNLIRFLNHGDKVKITLRFRGREMAHQELGMKILERLQADTADVAVVEQH
                     AKREGRQLLMVLSPKKTK"
     gene            complement(70129..72042)
                     /gene="thrS"
                     /locus_tag="ACF2SP_000070"
     CDS             complement(70129..72042)
                     /gene="thrS"
                     /locus_tag="ACF2SP_000070"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFD2357887.1"
                     /translation="MPNVKLPDGNVKHFEAPLTIYDVAHHISPGLAKAAIAGRVDGVL
                     VDTSYLIKEDCSLIIVTEKHEDSLEIIRHSTAHLLAQAVKALFPSAQVTIGPVIEDGF
                     YYDFAFERSFTPDDLSLIEAKMHELAKANLSITRRELPRNEAIQYFKGLGEEYKAKII
                     ADIPENEALSLYRQGDFEDLCRGPHVPSTGFLKAFKLTKVAGAYWRGDSNNEMLQRIY
                     GTAWADKKSLEEYLFRLEEAEKRDHRKLGKALDLFHFQDIAPGMVFWHPKGWTIYQEL
                     EHYMRNRLVDFGYQEIRTPQLVDRSLWEKSGHWANFRDEMFVTETENRHYAVKPMSCP
                     CHVQIYNHGLKSYRDLPLRLSEFGNCHRCEPSGALHGLMRVRNMVQDDAHIFCTEDQI
                     QSEVAMMLELVQSVYKDFGFTEIKYRLALRPEKRVGSDDVWDKAETALKLAMLGRNIE
                     WVDAPGEGAFYGPKIECSLSDCLGRIWQCGTIQVDFSMPARLEASYVAEDGSKQTPVM
                     LHRAILGSFERFMGILIEHYAGKLPLWLSPVQAVVLTISEKQNEYAEKVRKTLQKRGI
                     RANFDLRNEKIGFKIREHTLQKIPYLLVVGDKEVENCQVAVRTRDGIDLGVMTIDTIC
                     DTLTQEIIRKGSI"
     gene            complement(72170..72244)
                     /locus_tag="ACF2SP_000071"
     tRNA            complement(72170..72244)
                     /locus_tag="ACF2SP_000071"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72210..72212),aa:Val,seq:gac)
     gene            72558..72845
                     /locus_tag="ACF2SP_000072"
     CDS             72558..72845
                     /locus_tag="ACF2SP_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HFD2357888.1"
                     /translation="MWFKIIHSHVEIAIYAKPNAKKTKLMAISDDRLHIALHAKPQEG
                     EANNELLFFISQFFKIPKTQIELIKGKSSRHKLIRLPLSESVFCFLNNPKI"
     gene            73180..73665
                     /locus_tag="ACF2SP_000073"
     CDS             73180..73665
                     /locus_tag="ACF2SP_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2357889.1"
                     /translation="MEKVNLTKQFAYRLRDAMIAAGHNSHRSPSGVCIHKLAEMTGYS
                     LQICRKYLRGEVIPDPVKLIEIASKLNVSPGWLLFGETPNDPGTSKEHITISKSLLHH
                     IFTKAACLYNRELVEDDVSDFLMDLINDISLINATEEQSKKIIDLAISSVSHFRHSQG
                     T"
     gene            73933..74166
                     /locus_tag="ACF2SP_000074"
     CDS             73933..74166
                     /locus_tag="ACF2SP_000074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357890.1"
                     /translation="MLKAIGTHFNNIKHYDELYYLDQIKHGFPIGFSLATKINENYVI
                     YSIASHQSCAFTRELFSTHQEDFPSQYLILTSH"
     gene            complement(74311..>75711)
                     /gene="wipA"
                     /locus_tag="ACF2SP_000075"
     CDS             complement(74311..>75711)
                     /gene="wipA"
                     /locus_tag="ACF2SP_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipA"
                     /protein_id="HFD2357891.1"
                     /translation="SNFHEAYQQFVTIYEQYDDIVQEYLEIRTLLQLIQIKITNAQQR
                     ILDIEQKLSLAIDHQKEFSQSLLQLKKPIEANLQMAEKSKAGLEEKLSGLKTRLPSCI
                     ERFNKFMTQIEINDIKTLVRLLGDEVADRGSCDYFTLRILDFLYQNQIAIKIILSNHG
                     YEFIHAYEKLVIGQPFKPKGYIGDIQIKSFWGIQLLLEQSAITQEELRSLVERAYKPT
                     LKIIDYSLSEDGITLYSHAPIRFDSIRMAASQLGVTYNDSTKEALAETIDQLNAQLQI
                     YMKNNMLHLLFENNEINDPTNMTDEERNASPLIYLVWNRWNESKEVENARPGKYNGYF
                     VTYVHGHDPFQSPLTYVYNLDTLCGKYSRMGEEEQINKAFQFLTENRHTNVDKTASES
                     LLRNISRYKVLDSDEYTLKHKIPKTSPELATDILDCKIKESLIKLSLLGKPKAVSGSL
                     SVDQNTSIPNQAFLGK"
BASE COUNT        23693 a        16208 c        13802 g        22008 t
ORIGIN      
        1 caacatgatt accattatct ttttgagata ccctctaaca atggaaagca atgagtaact
       61 ttccgatatg gcaaaaattg ccaactggtg tatgtggggt aactggggca tcataaaacg
      121 ataacttaat ctgttaaccc ttatatatta attattactt attttacaga ttctgctata
      181 gaatttatta cagaaattac tcattggaat acacaaaatg acaatagcta tcttgtctac
      241 cggagatgaa ataatacatg gcgatactct taataccaat gcgcattaca ttgctcatgc
      301 cctttgctct gaaggcttac ctcttggatt acaagtggcc tgtagcgata aggaaaatga
      361 gattatagat agcctaaact tcctgacgga acaccatgat attctaattt taattggtgg
      421 gcttggccca acgaaggatg atattactcg ctttgctttg gctaaattta ccaaagaacc
      481 tcttattcag catgctgacg ctctggctca tatagaaaaa aaagcaaaca tagccaaaat
      541 atcactgaat caaggcaatt tgcagcaatg cctctttcca gccaatgcac gactttttcc
      601 taatccttat ggcacagcca tgggatgttc ttattcatgg aaaggtaaag tttttattct
      661 attgcctggg cctcctcgtg aatgccttcc aatgtttaat caggatgtac tgccgttatt
      721 attgcaaaca tccaggcata gcaataaaca aattctgcgc tggcgagttt ttggcttggc
      781 tgaaagtgaa atagctcaaa tccttgaaga agctctggaa ggtttagact gtcagacagg
      841 ataccgattg gaaacgcctt atctggagtt taaggttcgc tgcaaaagag aattgattga
      901 aaaaataaca tcaataatag accccatttt agccccgcac attatatcat cacttgaaac
      961 caaagcatct gatcaattga gaagcctgat cttaaagaat aatgaaccaa taactattat
     1021 tgatgagatt actggaggat tattgcaatc cctattggta agaccagaaa actaccattt
     1081 attaaatttt cataatctta acaggaccaa attatatttt cattgcagtg gattagaaga
     1141 ttattggtca caacaagcac cccaaggcac taccaaatta atcattaact acagcaatca
     1201 aatgcaagag ggtcgtgaaa cacatcagat tccttatcga agtgctatgg ttgtgcatta
     1261 tgccgctgaa tggctaagct tcagactctt tcatctcatc aatcaattgc attaaagcgt
     1321 agcaaagttc ttgtgttccc ttgctctcta tagcggaaat agaaaatact ttgtctttcc
     1381 acttcaagcc ttttattatg gattgaattt tttcttctct ttccttttca tcaggtaaca
     1441 tatcgatttt atttaaaacc aaccatctgg gtttgttgag cagatcggga ttatattgag
     1501 tcaactcatt aagaattgct ttcgcatcag cgacaggatc actcccatcc aaaggcgcaa
     1561 tatcaataac atgcagcaag acacaggtac gtgaaagatg ttttaaaaac cgatgcccca
     1621 atcctgctcc ttgagccgcg ccttctataa gacctggaat atccgccatg acaaaactct
     1681 tatagggtga aacacggacc acccctaaac ctggatgtaa ggttgtaaat gggtagtcag
     1741 ccactttggc ttttgaactg gagacggctc ttattaaagt agactttcct gcattgggaa
     1801 gccctaacag acctacgtct gccaaaactc gaagctccaa acgcaaattt ctggattcac
     1861 ccgggcttcc gggtgttgtt tgtctaggtg atcgattcac actgctctta tagcgagtgt
     1921 tacccaaacc atgaaaccca ccctgagcaa tcagcacggg aacacctggt tgactgatat
     1981 cagctaacaa ttcacctgta tcggcatcat aaaccatagt accgactggc actttgatgg
     2041 ttaaatcctc tcctttcttc ccagtacaat ttccacccat ccctgattgt ccattctctg
     2101 ctttgtattg acgagtataa cgaaaatcaa tcaaggtatt taaatcgctg ctcgcctcaa
     2161 aataaatact gcctccatca cccccatcac ccccgtcagg gccaccacga ggaataaatt
     2221 tttctctcct gaagcttaag caaccattcc ctccttttcc ggcttcaact ttaataagtg
     2281 cttcatcaac gaatttcatg cctaacctta gaataaaata ggacttacgc aaaattccaa
     2341 gctcgaagca aaaaatattt tcaatgaata cgtttagata agctaagtga ctgaattaaa
     2401 atatttttta gcaaagagat tgggattttg cctaagtctg taaaaaaaaa ccccgtttcc
     2461 ggggttaaac aaaacgaatt atgatgccat tgggtaatag cattgtttca gcttaactcg
     2521 cagcttcttc tcgttgctcc ggtaatatag tgacatactt acgatttttt tcacccttgg
     2581 tagtaaactg tactaacccc tctaccaacg catacaaagt atgatctcga ccacaaccaa
     2641 caccaggtcc aggatgaaaa cgtgtgccac gctgccttac aatgatttca ccggcattta
     2701 caaattgacc accaaaacgt ttcacaccga ggtactttgg attcgagtcg cggccattac
     2761 gagtactacc acctgctttc ttatgagcca ttgctatctc ctcttattta ccaatcgcag
     2821 tgattttaac ttgcgaataa tattgtctgt gccccatttg tttcatatgg tgcttacgtc
     2881 gacgaaattt aataatttta acttttttgt gacgaccatg atcaagcact tcggccttta
     2941 cagttgcttt agcaacaaaa ggtgtgccac aagtgacctt atcaccatcc actaacatta
     3001 atacttccga aaatttaatt tcattgccaa cattttcagg cagcatttca atttttaaaa
     3061 cgtcaccttc tttcacggta tattgcttac cgccagtttt aattaccgca tacataacta
     3121 ttctccaatt cgcctgattg gctatcacaa ttccaacaaa gtgccatatt ctatgaaatt
     3181 atgactatta cttcaagttc attttaaaat attgtatact tcaccaccct ttaccgattg
     3241 acttaatgtt tttgaggctt tcgagtaaat tttaatctgg tcgcggatta attaggagct
     3301 atagtcatat ttaagatagt gcctagaggg agcccttaaa tttttggagt ttttataatg
     3361 tcaactattc aattagaagc acaatcaaga acggatatgg ggaaaggtgc gagccgccgc
     3421 ctacgtcgtc ttgaaaataa agttcctgct gtcatatatg gcggaagcaa aaaacctatg
     3481 gcaatccatt ttagccataa taaagtgatt aaggcattgg aaacagaaag catttattcc
     3541 agcgtttttg acatcacagt agatggcaaa gtagagcacg ttattttgaa agccttgcaa
     3601 cgccatccct acaaaccaat cgtgttacat atggatttac aacgcgtttc cagtaaagac
     3661 attctggtta aattagtacc tgttcacttc ataaatgaag aacaatctcc aggaattaaa
     3721 gctggcggta ttgttcaaca taccatgact caagttgaaa tacgttgcca ggcaaaagat
     3781 ctgcctgaat ttattgaagt agatatgtct aaggtaggta tggatgatgt agttcactta
     3841 tcggatttaa aacttccaaa aggcgttcag ctgacagtgg atgtagccga tggtagtcat
     3901 gatgctcctg tggtgagtat acatgccgct aaagtaagtt caactgaact ggaagaaaca
     3961 cctgaagttc cagcatcggc tgtaccgact acagatcaag gggaaagtgc tgaataagca
     4021 cttccctgtt atatttatgc gaatactcag agcttaatcc agagtatttg cataaaatct
     4081 agtgctagaa tagtcaactc aaatccgctt aattcaaatt ttatagaaaa tctatggcca
     4141 ttaaactaat tgtcggttta cgcaatcccg gatcggctta tgaacaaacc agacacaacg
     4201 caggtgcgtg gttagtgacc gctttggccc aaagacataa ttcgcatttt aagattgaca
     4261 agaaaatgca agcggaatta actgaaattg atattaacaa ccacccatgt cgactagcgc
     4321 ttcctttgac ttttatgaat catagtggtc aaacaacaag aataatcagt caattctata
     4381 aaattgaacc cggtgaaata ttaattgttc atgacgagtt ggatttacct gttggacgaa
     4441 tcaaattaaa aacaggtggt ggccatggtg ggcataatgg tttgagagac atcactgctc
     4501 aattgggaac aggtgagttt catcgattac gtatagggat aggtcatcct ggacataaag
     4561 atctagtcca tcaatacgta ctcagcaggc cttctatgca cgacaggcaa caaatttatg
     4621 atgcaataga cagaggtata gcgataatac ctatagtatt gtcaggggat atggcccgag
     4681 caatgaatca agtaaacgct taacataatg aggtatgact catgggattt aaatgtggaa
     4741 tagtaggatt gcccaatgta gggaaatcga ctctttttaa tgcattgact aaggctggga
     4801 tagaggcggc caattaccca ttttgtacca ttgagcccaa tgtaggtatc gtcaccgttc
     4861 ctgattcaag gctggataat ttaagcaaca tagtcaagcc tcagcaggtg gttcatgcca
     4921 ccatgcaatt tgttgacatt gcgggattgg taaaaggcgc ttccagtgga gaaggattag
     4981 gaaatcaatt tttggccaat attagagaaa cagacgctat tgctcacgtc gttcgctgct
     5041 ttgacaactc ggacgttgtt catgtggagg ggcgagttga tcccttaagt gacatagaag
     5101 tgattaatac agaactggca ttagcggata tggaaacatt ggaaaaatcg ctattaaaag
     5161 tagggaaaaa cagtaaaagt ggaaataaag aagccatttt tgaattaaaa actctggaaa
     5221 aaattaaagc acatcttgat gccggatatc ctgtaagaac acttgaactt acccctgagg
     5281 aagaacaggt tagcaaacgc ctttttcttt taacggccaa acctgtactt tacatagcca
     5341 atgtcgatga taacggctat gagaataacc cgcttctgga taaagttcag gctctggctt
     5401 cccaggaaaa tgccagcata gtagcccttt gtgcagcaac agaagccgag ctggttgagc
     5461 tggatgaaga agacagacaa gagtttatgg ttgatttggg gcttagtgaa cctggattaa
     5521 ataaagtgat ccgtgccggg tatgaattgc tgggattgca aacctatttc accgcaggag
     5581 tgaaagaagt aagagcctgg acaatacgca agggtgccac agcaccgcaa gctgccgggg
     5641 tcattcatac tgactttgag aaaggattca tccgcgcgga agtgatctct tacgatgatt
     5701 ttattcgctt tggcggagaa caaggtgcca aagaagctgg aaaattgcgg cttgaaggca
     5761 aggaatatat tgtctgtgac ggggacgtca tgcattttcg ctttaatgtt taatcattag
     5821 tattctgcaa ctggtgattt ttcaagaatt gaaccaagga gcctcgatcc tctatcgttt
     5881 catcattgcg agtggtcaaa gcatcgaacc aggcattgaa gcaagcggaa gtaagctatc
     5941 aggcatgcgt cttactactc ttataccaca ctgactgttg cttagcttgc gatagctcaa
     6001 accgacgccg agtcaacaga agataaacga tttggtagaa aatgaatagc aaaccaactc
     6061 cctgtgcgta aatcaacctc aatgaggaca gtgtggctgt tttttaatct ataattgaat
     6121 taataaaaga aattaacccc attgattacc ggccaatgcg attgaggtag gtgaatgtca
     6181 ttactaaaga aaacaagaga gcaggattac tcaatcctgg ttaccactca agaccagcaa
     6241 agaaagcgcc atttacttaa tgttatttcc atagagcttt taattttggg tcttttgctg
     6301 ataataacaa acctttgcct gctaagagcc tggcttataa tcagtatgtt aatcgtgggc
     6361 attttcatct tactcggaaa tttaatctta attaaaaaga acttcaatct ggccttgagt
     6421 gggcacattt tgaatattct tgtgttgtct atcatcattg gaggcaatct catcgttgga
     6481 aaaactacca tgtcttacct gggatggttc tatgtttctc caatcgttgc ttttgctaca
     6541 cttggattag ccgggttagt catctatggt ctgctggcga ttggggcaat gtttcttttt
     6601 gtattcgaat tctacatccc catctattcc atttctcagg aatatgtatt ttttctggat
     6661 aataccaacc acctgtttat ttttgctctt attttcactg ttctttatca ttttctggtt
     6721 caaaactcac aatatgaatt gctgttaaaa gagcaaaatt atctgctcat tgccgataaa
     6781 gaaaagttcc attacctttc gcatcacgat tcattaacca acttacccaa ccgctcctac
     6841 tttcatgctc atcttcagac cctattagag aatactgaca ctacaaagga agccattact
     6901 ctttatttta tggatttgga taaattcaag cccatcaatg acgaatacgg tcatgagttc
     6961 ggtgatcagc ttttactttt aactagcaag cgtttgcaat cctgctttag gaaaaatgat
     7021 tttatagccc gactaggagg agatgaattt accgcgatta tcgttcataa accccatgat
     7081 caaattgctg aggctctaac tcttagaatt gaacaggaat ttgaacaacc ttttgtcata
     7141 aatggccaga ccattcattg caccataagc gttgggatag caaattaccc gcgcaatgca
     7201 aaaaatgcgg aagatttgtt aaaatgggct gataaagcga tgtattacaa taaaagaaaa
     7261 aaatatcaaa cgcttaaaca aacctcttaa ccaacattcc taatttaatc agtatgattg
     7321 ggccttcatt ttataaagca caggatgcag caatctgaca ttcacttgac aattaacttt
     7381 tacaacttta gcattcattg attaaatatc agagggtaaa tgaatgacta ttgaccgtat
     7441 acgagagctg gttagtgaag attttaacgc ggttaataat ttaattattg ataaaattca
     7501 atctcaagtt ggtttaattc aggacttgtc acaacatata gtcgaaagtg gcggcaaacg
     7561 tttacggcct ttggttgtcc tgttagcaag caatgcttgt ggatacaaag gaaaagacca
     7621 tatttctctt gccgctatgg ttgagttttt ccatactgcc actttgttgc atgatgatgt
     7681 gattgatgaa tccactttaa gaagaggccg acaaactgca aatagcattt ggggcagtaa
     7741 agccagtgtt ttagttggtg attatctttt tacccaatcc gtaaatctaa tggtagaggt
     7801 tggcattaaa gaaattctca atctactggc caatacttcc caccaaatta cctgtggtga
     7861 agtcaaacaa cttgccaatc gccacaatcc tgctttaacc cttgatgact atttcgacgt
     7921 catccgggcc aaaacagctc ttttatttgc tgcggctgcc tgcataggcc ctatactcac
     7981 aaatgcacct attgagattc gagatagttt atataattac ggtttacact tgggaaatgc
     8041 ctttcaattg attgatgatg ccttggatta ttgctcggat gctgccactt taggtaaaaa
     8101 cattggcgac gatttagctg atggaaaagc gactttacca ctgatacatg ctttacaaaa
     8161 tggtaatgca actcaacaac agcaaataag agaaagttta atgaaaggca gtctaagcca
     8221 tctgcctgat attttaattg ctattgagca aaccgaagcc ataccgttta cgaaacgaat
     8281 tgctgcccaa gaagtagact cagcactatc tgccctggaa atactgcctg actcagttta
     8341 taaagaagct ttagtcgatt tggccaaatt tgccttggaa agaaattatt aacctgataa
     8401 ttgcatcgat tctaataatt acacaaccga ttgataataa tctggtggag tgcggcattt
     8461 aaagcaggca gttttgcaat tggctcctcc ctgacacttc cttattgccc ctttcctaac
     8521 ccataagtcc agcataggtt gcaatgcggt cagatccaaa tgaaactccc tactcaactg
     8581 ttgagtgctt actacccctt cccggtagat aaaatcacgt atttgcagca gcattttgcc
     8641 tccccatacc atactggcta taacgaaata cagctaaaac aaaaagcagg ctcagtgaca
     8701 tagccaaaat ccagatcata ctttgttgag gatgatccag aaatttagcg ccttgataaa
     8761 agacaacgga agtggtgtaa gccacgacaa atgaccaaac aatggaagtc cacataaatc
     8821 gcttgtttgc ctcctggcga atcactgcca tagtcgaaac acaagggata taaagcaaaa
     8881 caaaaagcaa ataagcgtag gctcctacag ctccatcaaa acgtcgtgac ataatgccat
     8941 agacagattg agataactca ctatcagctg cactggcaga aacagggttc cacaatgcag
     9001 agcctaattc agacaaattg gcgggaatag acccaaaagc agctttgatg cccccccaaa
     9061 aatcaaagtg agcagcagct atttctccca catggcctac ttgggcataa agtgagttta
     9121 aggtgccaac cactacctct ttagccagca tgccagttaa taaaccgaca gtcgcgggcc
     9181 agttgtcttg atgaattccc attggagcaa ataatggagt aatccactga cctattattg
     9241 acaataaaga atccgtattg gcttctccgg agctaatccc tcctccccaa gtaatcgcat
     9301 ttaatcctcc cagaatcaca caaatgggaa taatcagctt acccgcacga taaacaaaaa
     9361 atcgtaatcg caaggaggtt tctctcagca atcttctgag tgagggtcta tgataagcgg
     9421 gtaactccaa aatcaaagga gaagcatgtc ccttcaacgt agttttgcgt aaaatatatc
     9481 cggtaaatac ggccattaaa atgccaatca aatacaacga aaaaacaaca ttatgcccgc
     9541 cagagggaaa aaaggccgcg acaaatactg cataaatagc cagtcgggca ctacaagaca
     9601 taaatggact catcatcaca gttaataatc gatcgcgctc cgaatccagg gtgcgtgctg
     9661 ccataatggc agggacatta catccaaaac ccacaatcat gggtacaaat gattttcctg
     9721 gtaatcccat ggctcgcatc gctttatcca cgacaaaagc agctcttgcc atatagcccg
     9781 aagtttccaa taaggacaaa aagaaaaaca tcgcggcaat cacaggaata aaagtaatgg
     9841 tggtattaat tcctttgcct accccattag ctacaagcgc gattacccaa tttggtgcat
     9901 ggagctgttg caacaaccaa ccactaccct gaacaaagat agtctctgta ctaatgtcaa
     9961 aaaaatcctg gaatgctccc ccaatattta tagcaaacaa aaacatcaaa tacatcatcg
    10021 caaaaaatat gggcaaagct aaaaagcgat gcaaaacaag tttatccaat ttagcagtga
    10081 agtgttcgct ggcatcacta tgcttttttt gaaccaacgt aacaatttca tggatttttt
    10141 gatagcgagc gtctgccaat aacacatcaa gattttgttc cgtttcttga agctttataa
    10201 gaagactctc ggtaaaagct ttctctccta tcaaagtatc cccttcggca agcctacggg
    10261 agaaatagta ggcaaaggaa tttttatacc cttttgagat cagctgattt tccaggtcat
    10321 tcaaaatttg ttgcgctgca accgataagg aaagttttag aggttttatc ttttgggagc
    10381 aatgaagtaa tgattgctga agtgccggaa taccaatgtt tttatgggcc tgaatgggaa
    10441 taacagaaca ccctaacaaa ctctcgagtt tctctgtatc aatagaaata ccccgatgct
    10501 cggcaatatc catcatgtta agtgctacaa caacaggttt acctaactca aaaagttggc
    10561 tggttaaata caaatgtctt tcaaggtgac aagcatctat aacattaatg atgcaatcgt
    10621 attccaaatc aataactgac tgagcagcaa tttgctcatc ctggcttatt ccttcggcat
    10681 tcgcaaccag agaatagaca cctggcaaat cagtgatttc aattaaatgt tctccaagca
    10741 aaaattcacc tgtttttttc tcaacagtta ctccgggcca gttgcctacg cgttgattcg
    10801 cattggtcaa agcattgaat agggttgtct taccacaatt gggatttcct attaataggg
    10861 catgagtcat agatgctcca acactaattg actcgcttca tctttacgca aagttaaaaa
    10921 tgttcccctt acttcaattt gtaccggaca ccctaatgga gccatcctca ctacagaaaa
    10981 ctcaactcca caagtaatac ccagggataa aagcctgcgt cgatactgca tctcagtcgc
    11041 accaaaactg accaatcgca ctttatcccc ttgttttaat tcactaatct gcataatata
    11101 taaacaatac ggtgattcat ttcattaatt ttaacacaaa cgatatcgag aatcattctc
    11161 aattaagaaa ttttatacca cattacagca tttcctttac caaacgccga ggatgacgac
    11221 tcagatagtc caccgattgc atctcaatta gacggctcaa cgttctttta aacgtttcct
    11281 tcatttcacc tttaacgtac aattcttctg caggaacatc cgctgagata atgagcttaa
    11341 tgcccctatc atacataaca tctataaagt gaataaacat aatcgcttgt gctgtatgac
    11401 tttcagtaag ctgataaaca tcacttagga agacattatc aaagcgctca gcaatttcta
    11461 aataatccaa ttggctacga ggaaaattac aaatcacatc aaaagcaaac caaatagacc
    11521 gtgccccgta tttaatatat ggaatatcac gattttgtac tgttataaaa cctttgcagt
    11581 cttcttcccg ggttattaga gcaaactgtt tttccattgc ttcttgtgtt tgctcattta
    11641 aaggatataa ataagcctct aacaagggtt gtcgacctaa acgataatct cgtttttgat
    11701 taagattcag aacctcacaa ttttttttaa taatctcaat agctggaata aatctctctc
    11761 tatgcacccc atttaaatac aactcatccg gaggagtatt cgaagaaaca accaaaacaa
    11821 cgccgtagtg atgcagtgct tgtaataatt cagccaaaat cattgcataa gccacatcgt
    11881 gaactaaaaa ctcatcaaaa catagtaaac gaatcgattt ggccatttct ttggcgatat
    11941 actgcaaagg attttttttg ccttgtaatc gtcttaattg agcgtcaatt tgctgcataa
    12001 aatgatgaaa atgaaatctc gccttttttt cctcatcaat atgctgataa aacaaatcca
    12061 ccaaataagt cttgccaact cccacaggcc catagatata aagcccttta ataggatgct
    12121 tttttctcca gggaaaccaa gagtcattct tcttctgtaa atcctcagca agcctctgca
    12181 tgtgttcaag gatttctctc tgttcaggat cgctatctat ttcacctcta taaatcgcgg
    12241 cttcatactg ctcaatcaaa ctcatgacag gcaaacctga tcacgaacag catccatcag
    12301 cttttctttt aatattaaaa gcttaccatg aaaaaaatga cccgtttcaa caaattcaat
    12361 gactggcaat acctgcgatg attggctggc gaactcactc accagttcaa atggcacgac
    12421 ctcatcttct ttgccttgta taataagcca gggatgagga agggggtcaa attctttgta
    12481 atcataatga tgcacaggag gagctatcgt tattaacaga gcatgctcac aataagacgc
    12541 cgttctataa gccacataag atccgaaaga aaagcccgca aaaatatttt gggcttgcgg
    12601 ttgctcagaa tgccactgcc tcacgagctt gcgcatatcg tcactctcgc caataccatt
    12661 atcataatgg cctccagact gtcctacgcc tctgaaatta aaccgtactg aaggaatatg
    12721 caactcctta aaggctctgg ccatcgtggt aactactttg ttattcattg tcccaccttg
    12781 taaagaatgt ggatgaccaa gaaaagcaac ataacgagta tccacatctt caggaacagt
    12841 cagtacggct tccagtgggc caactaatcc ttcaagcatt aattcatgct ctccgggagt
    12901 atttaatttg tcggtaaata gcataataac cctgccaatt taaagataat aacccaaatc
    12961 aatatggatt taagcaaaag agttaggcta ttatagcaat ggatgcgttg acccccaata
    13021 gaccttttgt atatctggtt tattttgttg gcagcgactt ttattgacct tcagaggagg
    13081 acttgatcca tgagacaggt acctgtatta ccatcattct tatctattcc aggcgcattc
    13141 tcctattcag ggagatcggc atgaaaatcc atgattttaa aatgaaaaag caggaacaaa
    13201 agaaaatttc catgttgacc tgttacgatt acccttcagc atgcatagta gcagaatcaa
    13261 atatcgattg tgttctcgtg ggtgattccg tagctatggc agtgcacggc caccctacaa
    13321 ccattatggc cactatcgaa atgatggaat tacataccca ggcagtagct cgtggtttag
    13381 gcaaacagtt tttgattact gacttacctt ttctcggaca taaatcatct caaggtcata
    13441 cagtagaaaa cgtaaaaagg ctgttacaag ctggagccca agcagttaaa atcgagggcg
    13501 ctgataaaga cacctgccaa acgatttccc atttagttaa tgccggcatc cctgttatgg
    13561 gtcatatcgg cttaactccc caatccattc atcaattagg cggttataaa gtccagggaa
    13621 aaaacagcga acaggcagaa actctattac aacaggcagc caccctggaa caagccggct
    13681 gctttgcggt agtcattgaa tgtgttccgc aggacctggc aaaaacgatt accgattcgt
    13741 tggttattcc tactatagga attggcgcag gcccaggaac tgatgggcaa gtccttgtct
    13801 ggcacgacat gctgggttta cagacttcat ttaatcctaa attcgtaaaa aaatatttta
    13861 gagccaaaga ccattttatt gaagccttaa acagctatgt ccagcaagta caacaaatgc
    13921 atttcccagc taatgaacat tctttctagg aaaattatcc atgcaaattt ttcataattt
    13981 aaatgagtgg attcgcttta gaaattccct gtctcctgat ttatccctgg ggtttgcccc
    14041 aactatgggt aacttgcatg ctggacatgc ttctttgttt ttagccagta gcaaagaaaa
    14101 tcactatacc gtctccagtt tgtttgttaa cccgacccaa tttaataacc ctgatgatta
    14161 caagcattat ccacgcacag tcgatgctga tctcgaatta atgactcaga atggagttga
    14221 tttttgtata ctccccaatg aaaatgaaat ctataccgat ggctatgctt atcaagttca
    14281 agaaaaccgg cttgggcaat tgatggaggg aaaacaccga ccggggcatt ttaacggagt
    14341 cctgaccatc gtcatgaaac tctttaattt ggtcaaacca acctgtgcct attttggtga
    14401 aaaagattac caacaactgt tgctaattca aggaatggta aaagcattgt tcatggatat
    14461 tgaaatcaaa tcttgcccca cagtaagaga aaaaagtggc ctggcctgca gctcacgcaa
    14521 taacaggctt actccaagcc aaagagaaat cgctgatgaa tttgcaaaaa tatttcatca
    14581 aaacaaatcc tctgccatga taagcaaaga attggaagcg cttggaatta ctgtagaata
    14641 tattgaagaa ttccagggca gacgatttgc tgcagtaaaa attggcgata tccgtctcat
    14701 tgataattat ttgctatgat tcaaaccaat tggtgcgtga taacaggtgg accttcctca
    14761 gggaaaacca ctttaattaa tcatttggca gagttgggat acaaaactgc gccagaaata
    14821 gccagatttt atatcaacca gctgctgtct catgataaaa aaactctaca agaagtaaaa
    14881 catgacatcc tttggttaca aagaaaaatt ctcaatatca tgttaagacg agaaagaaac
    14941 ctgcctaagg acgacttaat tttttttgac cgtggtaccc cggatagtat cggctatttt
    15001 cgttttcatc atttagatga ggctcatgtg gttagagctt gtcggtattt acgctataaa
    15061 aaaatttttt tctgtcatcc cttgcctgtt gtccctgata ccataagaga tgaagatgat
    15121 gtcaccgcaa agaaaattag cgattacatt tataatgcct atgtcggcct taactaccct
    15181 gtcatagaat taccagcatc ctcaataaaa gaacgcctgg aaattatttt atcccatgtt
    15241 gatacctaga gataaacctg ttctaggttt tatttaaagc gattgccgct gcaataaccg
    15301 ctgccttgtg ttgaggcaag gatttatttg aatactttaa ccattcgggg tgtgcctcca
    15361 ctaaaccggt attgaatacc cctttctgaa actcctctga ttttatcatt tctaaataat
    15421 aaggaatggt agtttttact ccaaacatgc gcatttcctc aagagctcga cgcgctcgtc
    15481 gaagtaccga gggccaatca aaagcccata cggtcaattt ggcacacaaa gaatcataat
    15541 ccgggggaat ctggtaacca gtatagattg cgctatctgt cctcacaccg ggtcctcccg
    15601 gggcgtaata tcgggtaatc cgcccaaaac tgggtaaaaa atcgttttga gggtcttctg
    15661 cattaatgcg aaattcgatg gcaaaaccat tgtatttaac ttgttcttga gagatagtca
    15721 gacgctcgcc agctgcaatt cgaatttgct cctggactaa atcaatacca gtaatctgtt
    15781 ctgtaattgg atgttcaacc tgtaaacgag tgttcatttc catgaagtaa ggttgattat
    15841 ttttatccaa aataaattca acagtacccg cattggtata ccctacctcc ttggctgcct
    15901 tgactgcgta tcccaaaagc acttgacgtg tcgcctcatc caattgggga gaaggggcta
    15961 tttcaaccag tttctgatga cgcctttgta ttgaacaatc tcgttcaaac aaatgaagta
    16021 ctgttccatg atgatctgct aggatttgta cttcgatatg gcgtggattc gctatgtatt
    16081 tttccatgta gatatgggag tctccaaatg atttttctgc ttcagattgc acacgagcaa
    16141 actgttgctt tatctgactg gcatcatgac ataatctgat acctctaccc ccccctccaa
    16201 aagtcgcttt gagcattaca ggatacccta gagtttctgc gcaatgtaaa gcgtcatcga
    16261 tagtttccaa attccccttg cttcctggta tggtgggaac ccccgctttt atcatcgctt
    16321 ctcttgctgc aatcttggag cccatagtgg ctatcactgg agcagacggt cctataaaaa
    16381 cgatacctgc cttattacaa tcctcagcaa actcggcatt ttcagataaa aaaccatacc
    16441 cggggtgaat tgcttcacaa ccggccacct tggcgcactc aataatacgc cgtccattaa
    16501 gataagcatc cagaggtttt ttactaagac agtgagaatg agaagctctt ttgacatgca
    16561 gggcaaatct gtccacttca ctgtgaatag taactgctgc tatccccatc tccttgcaag
    16621 cacgtactat tcgcagggct atctcaccgc gattagccac cagaattttt ttaaacatta
    16681 aaaatctctt gtaaaaactg gctcattgct tgccatgaac ggcgcgcagc aacctcatcg
    16741 taaattaatc ctaaatgttc atcatgcgct tgaggattag taaaagcatg tttcacctgc
    16801 ccatacatat gcatttgcca atccacatta gcttctgtca tttcctgaca aaaagcatgt
    16861 acttgttccg gtttcgccat aggatcatca taaccgtgca aagccagaat ttttgcttta
    16921 attcttccgg atgctagatt ctcaggctta ttaagcaaac catgaaaact tactgctcct
    16981 cgaatatccg caccactcct ggctaaatca agggcacaca agccaccaaa gcaaaaccct
    17041 ataatggcta tgcgattgtt atcgactacc gatattgatt taatggcttc caatgcggca
    17101 tacaaccgct ccctaagcaa agagcgatcg ctgtacaagg gttgcattaa tgccattttt
    17161 tcatcattgt tagaaccaat ttgtccttgc ccatacatat ccaaagcaaa gcctatatag
    17221 cccatctcag caagttgttt tgctttaagg caagcaaact cacctcttcc actccaatca
    17281 tgagcgacta ttacggcagg ccgagattgt ttaattccct cttcatgcgc taaaaatccg
    17341 tgtaactcaa gatcatcatg ttgataaata taattcgatg aatacataaa atatctccta
    17401 aattatttta aaatagtcta gggcaatatc cgtaaaagtc cagtaattac attagtttag
    17461 cgtaatgcta ttctttgtct tttgttcctc gtggctaagt aaaaatagtg tcgtccaata
    17521 aaaggatatc aaattgaatt aatctaatgc tcttgcatgt ttcgccaaaa catgagtcca
    17581 tgagtcagta aatatcaata tgtccactaa ttaactcaaa ttatggattt ggtctaaaat
    17641 aaaaataaat tatgtgagga atgttgccat gatgactgat tcgttaatac acataaggga
    17701 tttgttacat gctcaatttt gctaccaaat gtttattacc cccattcatc ttcctatcga
    17761 taaaaaatac agagaatttg ccaaaatggc ctgtgaattc atagaaggaa gacgggatga
    17821 aatcatacac agagaatatc ctcgtcatca tgttttacat cgatttactc ccgcgaacaa
    17881 accaaatgga aaaaaaatat tgattacgca tggctggatg tctcgtgctg cttatatggt
    17941 tcgcttaatc aggcttcttc ataaagaagg atatgaagtc tatgctatcg attttcctgc
    18001 ccatggcgaa gctaaaggaa ttcagcttcc ctggacagat gccattgcta ttatcaaaga
    18061 aacgattaac caatttgggc ctttttatgg attggttggc cattcttttg gtggttcaat
    18121 gattttaaat actttgaatc tggcaggcca actaccagaa tggcaattaa attacaagcc
    18181 ggaaagagcc attttaattg catcgccaac acaaatgcgg actcctgtca ataaaattgc
    18241 cagacgtttt aaattaagcg ggcatgccta tttacaactt cgccaattaa tccgtcaaca
    18301 agcatcggta gatcctgaga gaattcgttt aagtcatttt atttcccaaa cccctgatac
    18361 ctattttcta tgtatccatg gagaattgga tgccactatc aaccccaaag aatcgataaa
    18421 tttttgcaaa tattacaaaa atgcccgcct gtctctttta ccagacgctg atcatgttag
    18481 tgtgttaatg gatgagcgtg tcgaacaaat cgctttggat tttttaaatc cttaatatga
    18541 caggggaatt ttgatccacc tggattacgg ctttacctaa tccaggtgga tccagtagat
    18601 aggcagatca ttgtatttgc tgaagcaatg tgcttcacat acagaggaat tggcttcaaa
    18661 attttgtaat taaccttcca tgtattgacg gatttttttc atggcatttt tttcaagttg
    18721 cctaacccgt tcagcagaga caccatattt ttctgccaaa tcatgtaacg tgagtttttc
    18781 ttcagcaagc cagcgttgtt gtaaaatatc ctggctacgt tcatccagtt gttccatagc
    18841 aaacagtaat ttttcacgcc cctgatcgcc tgtatcctca ttctctaaca aaacagcagg
    18901 atcatcgttc acattaaaca aataacgctc aggcgctttg taagcatcat cattatcatc
    18961 aacatctggc gcatcataag atgaatccat aacatttaaa cgttgctcca tcaacaacac
    19021 gtcttctcgg ctaacgccca aatctttggc gacagcgtca acttcttcat tgctgaacca
    19081 acccaatcga tttttcattt ggcgcaaatt aaaaaataat ttacgttggg ccttggttgt
    19141 agcaacttta actattcgcc agttacgcag cacaaactca tgaatttctg ctttaatcca
    19201 atgaacagca aaagaaacaa ggcgtacgcc catcttgggg tcaaaccgtt ttacggcttt
    19261 catcaagcct acattacctt cctgaattaa atcacttaaa ggcaatccat acccaagata
    19321 accgcgggct acacgaacta catagcgcaa atgcgcaaga actaattgtc gagcggcttc
    19381 tatatctcct tcagaatgaa aacgctcggc gtatgcaatt tcctcttcca aagtcagcat
    19441 tggaatttga tttactcgat ggatataaga atcaagacta ccaacaggta gattcattgc
    19501 agcaagttgc aactgttgac tcatactact tcctccggtc attaacacta gaatagtatt
    19561 aaaatttaca tcctattaac tgataggatt caccaaaatg aatgctaagc tataaatttt
    19621 caaaaactgc aaattaagta aaacttcata acttagcact ctcttcatga tgtttcaatt
    19681 ttggtcatta tttcagcgat ccttggcata tttataaatc gctaaacctc ttatataaaa
    19741 attttttacg gtttataagc acacaccaaa ctgaccgaat attgaccaaa accaatactt
    19801 catcaactaa tattatacat tatttgtagg gttctataga agccaattgc cgcttaacag
    19861 acaacaaggc tccaagccac ccgagtataa tagcaaatag cacaagtagc aagatttgcc
    19921 gtagtgacaa acaagctaaa ggatagtgca tttgatagac attagccagc tgattcactg
    19981 cgacgcctag ggttagaata aaaatattaa ccagaaagat ggctaaaagc gcgccagctg
    20041 ccccatacca aactccagaa tataaaaaag gacgaataat gtaaggatct gttgcgccaa
    20101 tcagttttaa aattttaatt tcttcttgtc tattatgaat gtctagacgt aaggtattgc
    20161 caataataaa tactacagcc aaagctaata acgccattaa agcatttgct gccttgccag
    20221 aaaaacccaa gatagcgtgc aatcgattaa tccactccat atccagtttc gccaacgcga
    20281 cttgtggaaa agcctgaagt tttcgagata ataaatctaa ttttgcagga gaatctataa
    20341 ctaatgcggg aactacttca ataactgcgg gcaaaggatt ttcaggaaga tagcgcataa
    20401 tgtcttgcat gccttcttgt tgagttaact cggataatcc atctgctgca gatttgatat
    20461 ttgcctgacc aactccttca gtagtgcgta ctttttcaag taacgatgtt tgctccgctt
    20521 ccggcaatga aggtttcaaa aacagggaaa tgtgcccccc tcgctgccaa cgattggtta
    20581 gttcacttaa attgtcagtg aatacccaaa aaagtgtagg taacgccaaa gcgatggcaa
    20641 taacaatgac ggtcatcatg gttgctatcg gtttgcggca gaaaagattc aagctattgg
    20701 ccgcggcttg caaatgatag gcaagaagag attgtgatcg ttttaacaca gccttcctcc
    20761 tttcagcatg acgatgcgat gtttcattcc ggctattaaa gccaaatcat gggttgcaat
    20821 taatatacta actccaacct gattaaattg ttcaaaaatc ttcattatct cagaagaaag
    20881 ttttggatcc aggttaccgg taggttcatc tgccaataac aatgcaggct tatgaactac
    20941 agcacgagcg ataccgactc gttgctgttc accaacagac aggtgaactg gtaacatttt
    21001 ctctttactc aacaatccca ccatatccaa agcagcatgc acccttttgg caatcatagg
    21061 ataagcaact ccttgaattt gcaagggcaa ggcaacatta tcaaaaacag ttctgtcatt
    21121 cagtagctga ggagattgaa atgtaatgcc caactgactg cggtgagcag caacatctcg
    21181 ctttttgaga tgatttaatt tcaaaccatt gactgttaat tgaccagacg tcggccactc
    21241 aagaagagca atcaacttga gcaaggtgct tttgccagca cctgaatggc cagtaagaaa
    21301 gaccatttct cctttttgca aagaaaaatt aacttggctt aatgcctcaa agccccccgg
    21361 ataacgctta ctaacctggt caaatgtgat catcatcgaa tattgcacta acaaattgcg
    21421 ctgcgtcaaa gggcctcaaa tcttcaatgc cctcccctac ccctaaataa cgaaaaggta
    21481 tccccaaatc attcgctata gcaaataata tcccgccttt ggctgtccca tcaagcttgg
    21541 tcatagtaat tccagttaat tgtactgctt catgaaactg ctttgcctga gccaaggcgt
    21601 tctgcccaat actcgcatcc aataccaaca tggtttcatg aggtgccttg ggatcgagtt
    21661 tctgcatgac tcttttcact tttttcaact cttccattaa attgctttga gtatgcaaac
    21721 gcccggctgt atcagcgatt aaaacatcta tttttctcgc tttggcagcc tgaagagcat
    21781 caaaaattac tgaggcactg tcagcgccgg tatgctgggc aataacaggt atgccatttc
    21841 tttcacccca tacatgcaat tgctccaccg cagccgcccg aaacgtatcg cccgcagcta
    21901 acatgactct ttttccctgc ttttggaatt gtttggctag tttaccaatg gtggttgttt
    21961 taccggcacc attgacacca accattaata tgacaaaggg agaaccgtct tcagtttcta
    22021 tggaaagcgg tttctcttcc tggctcaaaa tcgtttgcaa atttgtcttt agagtctgat
    22081 aaactgcttc accattagac agttgctttc ttgccaagcc ttcatttagc tgtttcaata
    22141 cgtcttgagt ggtgtccaaa cctaaatcag cactaattaa taaggtctcc agctcttcga
    22201 gtaactcctg gctgatttct tttttaccta acagcagccg acttatgcct tcccccaatt
    22261 gatgacgggt tttgctaagg cctttcctaa accgggcaaa aatactttct ttgggcgaag
    22321 gctctttgac agtcagttcc tggggttctt caagcctgga ttccatgctg ctttcggcat
    22381 gatcttgttg ctcttcggta tccaaatttg gttcggaatt ttgatttcgt ttaaaccatt
    22441 taatcatttt agtgtacaat tccttaaaaa tatgaaattc tatcatcttt ttagctttga
    22501 ggtgacattg tgcacaaatt actcatttct cttatgatgc tactctcatt tcaggctttt
    22561 agccaagtcc aggagtatac gttaaacaat ggtttaaaag tactggtaaa agaagatcat
    22621 cgagctccag tcgtagtatc catgatttgg tataacgtgg gttcagcaga tgagcctgta
    22681 ggcatcactg gtgtttctca tgccatagaa cacatgatgt ttaaaggaac ttccaaatac
    22741 ccggtagggg tattttccaa aacaattgct gcattgggag gtcaggaaaa tgcttttaca
    22801 aacaatgatt ataccgccta ttatgaaaaa ttagatgctg atcatctggc tacaagcttc
    22861 gaattggaag ccgatcgcat gaataattta cagcttaata gcgaagaatt cgctaaagaa
    22921 attaaagtca ttcaagagga aagacgttta cgtactgaca ataacccgca ggcacttgct
    22981 ttcgaacgtt ttctggccac agcgcatctt acagcaccat acaatcaccc ggtgatagga
    23041 tggatgaatg atttgaaaca aatgaaagta gaggatttaa aaaaatggta cgaaagttat
    23101 tacgcaccaa ataatgccac cttggtagta gtaggcgatg tgaatcctga gaaagtacat
    23161 gccttggctg aacgttattt cggttcaata gccaagcgtc ccatagcgtc tcgtaaaccc
    23221 cagcaagaac cctcagcgct cgggaaaaaa atggtttaca ttaacgcacc agcgaaattg
    23281 ccattactgt taattggcta tactgtacct agtgtaaaaa ctgctaaaaa taactgggaa
    23341 ccttacgcct tggaaattat cgccgggata cttgatgcag gggaaagtgc gcgctttgcc
    23401 aaacacctgg tacgaggcaa tcaagtagcc actggtgcag aagcctatta taacctctat
    23461 tcaaggtacc aaagccaatt tattgtctat ggggcgccta gccaagatca tcaaataaaa
    23521 gatctggaaa aggcattaat tacggaattg gaagccttaa aaaaagcgcc tgttagcaat
    23581 caggaattac aaagggttaa aaatcaaatc atagcacaaa aaacttttga aaaagattct
    23641 atttttggtc aggccatgga actgggattg ttagaaacaa taggtctggg ttggaaaaat
    23701 acggaaacat acaccaaagc catcaatgaa ataactcccg agcaaattca gcaagttgcg
    23761 caacgatatt ttcaagaaaa taacatgact gttgcagaat taaagccaat caggcaagaa
    23821 gaggtaagac catgagtgtt ttaagagctt ttatcatttc gtttagtgta attttttctc
    23881 acaatctatt cgccaattca ttcaaaactg aaaaatggca gactaaaaat ggggtgcgag
    23941 ttgtatttta tcaagcaatg gaagtaccta tgcttgatat tagtttggca tttgctgccg
    24001 gctctgccta tgacggtaag tattttggtt taagcgcatt aacaaccaat ttgattaatc
    24061 aaggcaactc cggtaaagac gcaacgacta ttgcggaagc tttagcagac accggagcgc
    24121 aatttaacgc cgaaacgagt agggatatgg ttgtccttag tctaagaact cttactagca
    24181 aagaggcttt gcagcaatct accaaaacct ttagccaaat tattagtcat cctgattttc
    24241 ctaaagaagc cttcgcaaga gaaaaagatc aacttctcat ggcagttgaa caaacagaag
    24301 aatcgccaga tgatgtagcc atccagaact tttttaaaac cttatatcaa gaacatccct
    24361 atgcccatcc tgtgcatggg acagttgagt cactaaatgc cattaaagaa aatcaagtca
    24421 ttgattttta taaaaaatat tttgttgcta aaaatggtat attagttatg gttggtgcta
    24481 ttgacagttc tcaagctcat caattggcag aacaattggc tcagaatcta cctgcaggag
    24541 aaccagcacc aacaattccc aaggcctctc agctggccga tgcggaaaaa attaatgtac
    24601 cgtttccttc ctcacaaaca gtagtccgct tgggacagat aggaatagat catcataatc
    24661 aaaattattt ccctttaatg gttgggaatt atattttagg tggaggaaca ttagtttcca
    24721 ggctgggaac tgaggtaaga gaaaaaagag gcttaactta cggaatcgat agtcaatttg
    24781 tccctatgct aggtgaagga ccgtttatca ttagtttatc aaccaaaaac tccgaggcca
    24841 gaaacgcact gcacattacc caggataccc tgataaaatt tatcaaaaat gggcctaaca
    24901 aagaggaact gacttcagcg aaacaatatc tgacaggtag ttttcctctc tcactaggaa
    24961 gcaataccaa tattgcaaac ctgttattaa gaatggcgtt ctaccattta cctgataatt
    25021 atctggatac ctatgtcgct aaaatcaacg cggtaaccga tgctgaaatc aggcaagcat
    25081 tccagcaaca ggtgaaccct gaaaaattac tcctggtaac ggttggacaa tcttgaaaca
    25141 ggtaatacgg attatcggag gattatatcg gggaaaaaaa atacatttcc ccgatgtaga
    25201 tggcctgaga ccaacaccag acagagttcg ggaaacactc tttaattggc taatgaatga
    25261 tattagaaat gcccgctgtc tggatgcttt tgcaggtagt ggcgcgttgg gatttgaagc
    25321 ctattccaga ggagcgtctc atgtcgtttt tatcgaacaa tctcccaaag ctcataccag
    25381 cttgcaaaat accattcgtc aatttgatac ccccaacttg caccttataa aaactgatac
    25441 gttaaattat cttaaacaaa gcaccgagca attcgatctg atttttttag atcctcccta
    25501 cgctctcaac tatgttcctc aatgtcttga tcatatcata acaaatcacc tacttgttaa
    25561 aggaggtctt gtctacattg aatcatcatc ccctatcgaa gtaaaaacag aactttggaa
    25621 gcaattgaaa ttaaaacaag ccggccaagt catttatgga ttatttgaaa aactctaaca
    25681 aacaatgtct tgacaacaag ccttcctcct gtttcaatca aactaatatt tccaaatggt
    25741 aaagaacatt gaatgaacaa caataagatt gtcataatgt ttattttttc agcgcttctt
    25801 gctggctgcg ctggaacaat gaaatttaaa aaaccaccca tcaacaatcc aagtgatgat
    25861 gcgactatta aattagcaga agctgccgtt tcagtcagtg actccatgct tgaaatggca
    25921 aaagttgaaa aagtaattac tccaccaagc aaagacaata ccttaactat acctaatgct
    25981 tataacttgc aagcaagagc cagtgtcgat tggtctggtc cgattgaaga attgactgcg
    26041 cgtatcgcca aagcagcaca tttcaggttt cgtgttttag gcaagtcgcc ttcagttcca
    26101 gttttaataa gcatcagtac caaagatgag agtcttgccg agatccttag agatattgat
    26161 tatcaagctg ggataaaagc cagtattcat gtgtatccta acagtcaggt cgtagaattg
    26221 cgctatgcga aaatttattc ttagtttatc tattttgcta tcagccctcc tggtagcctg
    26281 ttcttctcgt aatcattacg gggatactgg ctcattggca ggccttcaag cgatggctga
    26341 ttcaaaatac acccgagctc aaaaaaaaca aaaaatgggt aaaattcgcg agatggccct
    26401 taaggaaacc gctttaagtg ttggcgccca ggctgggtta gcctggagag caaaaataat
    26461 tgacgagcaa ttaaacaaac aggcaaggaa tcttgatgca atttatgatt ttaattccct
    26521 ggtcttagag cataatattt tacctcctgt attacttgag ggaaggaata ctttgaatct
    26581 tgcagatgct caaagtatcc gtgtttcaga tcgcacctat aaagtcgcaa aacaagctca
    26641 tttcattacc actccaccta cctggcggca atatttgtgg atggattatg tgaaacctga
    26701 agctcccaat gtcactctac tccccaaaac caaggctgaa aaagaaatat ggtgcatcta
    26761 tactgaaaga ggatggaaaa atggtataga tcaggcaaac accattttgg aagaaaacat
    26821 tgcccgcatt aaagaagatt tcggcggtat gattctttat agaaaattac tggctatgaa
    26881 catggtatct cctccttatg tttctcatac tgacttgggc gtaactgggg atggctcaga
    26941 aattcatatc gatgacaggg tcttacgcat cacggcgtta cctgaactta atgtcaatag
    27001 tgcggaatgg agagctgcgg ttgccaaaga tgaaaatgct cttgaacgtt tcaaaaatat
    27061 ggaaaaactg gcaaatcagg ctaaaattgt tataacgaac aaatcctggc aacctattat
    27121 agcaccagta agttaatgtc tatggataat attaatttaa tgcccgatga gccaactcgt
    27181 ttcactccag tattcatgga cagaatgctg gagcatgcag aaagcctgaa tgcttcagat
    27241 ataacaatcc aaactggcga acccattttt gcagaggttt atggaagact ccttaaaatt
    27301 acaaatcgac gtctttccaa tacagaatta ggcgacctta ttaattccat ttatggcccg
    27361 aatgccacga cacaattact ttccggtaaa gatatagata ctcattatga gtttcgaccc
    27421 aaccgtggtg tacgctatcg atatagggtt aacgcgactg cctgcctggt tgaaggtcat
    27481 gatgccattc agatcacatt aagaactatt ccaaccacac cacccaaact cagtaccatg
    27541 agcctccctg ataacattat tgaagcgatt gctccacagg aaggaattgt gtttatcaca
    27601 ggcgctaccg gttcgggcaa atcgacattg ttggcctcca ttattcgaga gcttattgaa
    27661 acatcagact caaaccgcaa agtattaact tacgaatccc cgatagaatt cgtctatgat
    27721 gaaattgaaa caatttccgc tgtagtaagc caatcagaga ttccaaggca cttacccaac
    27781 tttgctgatg gagtaagaaa cgcattaaga cgaaagccga gattaattat ggtgggtgaa
    27841 tgtcgtgatg cagaaaccat cagcgccgct cttgaagccg ctttaacagg gcaccctgtc
    27901 tacacaaccc tgcatacttc tggtgttgca gaaacgatgc gacgtctggt cacatctttt
    27961 agcggggaag agcgtttggg aagaaccatc gatattcttg aaacaattag gctttgcatt
    28021 tggcaaaaac tcgtcccaac cgttgatgaa agacgtgtag ccttaaggga atatctggta
    28081 tttgatgagg aagtgaggga tattctattg gagggagacc ctaacgaagt gacttcagcc
    28141 acacgtaagt tagtgaggca aaaagggcaa ttaatgactt gggacgcaaa aatgaaattt
    28201 gaacagggga ttatcagtga gcgagtttat aaattaatca ttgctggggc caaagaatat
    28261 caacaataat caaaattaat ctgcttgcac ttgccataga aaatcattga tataccgcat
    28321 ataaaacaat caagatcaag gattcgattc atgatatttc acaggaaatg cctatcccat
    28381 ttttttaatt tatttatttc gttaatttta ggctcctctc tcaattgggc ttccgtcaat
    28441 cagcacagca cagattcgcc aacactcaac attaaaattt taggcattaa tgattttcat
    28501 ggtcaaataa gcagtggaag aactgtaaaa aacgaacctg ttgggggggc agctatcctg
    28561 gccgcttata ttaaagaagc ccaattggga atggaagatc gatcaataat taccatcatg
    28621 ggtgatttag ttggagcctc ccccccttct tcaggactgt tgaatgatga gcctagtatt
    28681 ttattcatta atagcttggg aaataatcaa tgcaagcccg aatcaagaat gaatcctctt
    28741 tgcaacatag ttgccacggt aggaaaccat gagtttgata aagggcaaaa agccatgttt
    28801 gatttaattt atggtaccaa taatccaccg actgattcat ggatctcgtt acccagttat
    28861 ccgggctcat cttatcccta tgtttctgcg aatatcgttg acgccaagtc agaacaacct
    28921 ctcttcccgc cctacataat taaacggata cataatattc ccatagcatt tattggggct
    28981 gttttaaaag atgcggcgga ctccatgttt cctgcaaatg cagaaggaat taaattttta
    29041 gatgaagcag acagtattaa tcgttacatc ccggaaatta aagcgaaagg agtcaatgtt
    29101 attattgtcc tgattcatga aggtgggaat caaattcctt atgagggaga tacccaaact
    29161 gcctcagcag ttgaaggcca tattaaagaa atagtctatt cccttggtga tgaaatcgac
    29221 gttgttatgg gtggccacac ccatcaattt ctaaatgctt tccttcgtaa tcgcaatggg
    29281 cacgagatcc tggtcacaca agcgaatagt tacagtgctg catttgctga agttaccctc
    29341 caaatcgata ccagaaatca tgcggtaaaa aacaaatcag ccaaaattat tacaacttat
    29401 gctaatcgat ggccaggaac aaatccggat acacaagcat tggaaattgt taaattagca
    29461 gaagataaaa ttgggccaat aataaatagc catgtaggca ccgcagaata tgatctgcta
    29521 agaaaacaaa atgaagatgg agagtcgagt ttaggaaatc tgatcgcaga tgcttataga
    29581 actgtaatga ataccgatct gggcatcact aatcccaatg gaattcgtga tgatttaaaa
    29641 gcaggggaag ttaactgggg aaatatctat tctattttac ccttttccaa caggatagtc
    29701 ataattacct taactggaaa tgatatttat gacttgttag agcaacaatg gatgggatct
    29761 tatatcaata tgttacaaat ttccgggttt gcctacacct atgattcaca tcaacctctg
    29821 ggacataaaa taattgaaat tagacatcaa aacaaaccat tgcttagaga aaagatgtat
    29881 accatagcca cttcagattt tcttgccagc gggaatggag ttttttcagt tatgaaaaga
    29941 ggaaaaataa tccatattgg tcaaagtgat catgacacgg taatcgctta tattaaaagt
    30001 ttacctcaac cctttcacgc gtccattgag gcaaggataa aagaatcaga gactggatta
    30061 aaaacaggag ttggatagac taaatttgtt ttaccgtcag cccctgtttt tattatttat
    30121 taattaagtt ttgctattat tttatgcatt ctaatccagc acatagtaga atttgttttc
    30181 gctaagtcct caagttcatt atgtaattgt ttgatttcct cccttttagc tatgccaagc
    30241 tctaaaagct gaggggtcaa atcatcaagc gccattgaat aaattagttt ttctttgttc
    30301 gttctgagca agggttgaaa taaagaacaa tgctcaattt caaaaccgca ggatgaaaac
    30361 tcttgctcta atcgatgcgc tatctgaaaa tcccgattgt ttttgctaaa gcatgacctc
    30421 gcccattgtg taaactggtc ataggagggt gaaggaggag agcaaaaggt agaatcaaat
    30481 aaagagggct cttcacatac gacgaaacca ccagaattaa gacaattttt catttgaaga
    30541 atgacagagc gcgcatcagc cacatgatgt aaaaccattc gacaataaat caaatcaaat
    30601 ttttccccaa gatctgaaag atgattaaca tcccacacct taaatcgtat attgctatag
    30661 gttctggttc gttgttgagt atgatccagt tgatcctgac tcaaatctat agtaagcaaa
    30721 ctgccctccc ggccaattaa tttggctaat tcgagggtca tatcaccaga gccacatcct
    30781 atctccagag cagtctttgc cgctgaaggc ccaacctcag aaagtaaatc caggctgctc
    30841 ttggcataca attgatgttg cagagaaaga cgctctctcg ccttgtcatt ggtatttaga
    30901 atgtaagtac tgggcataat atatccttat atttgataaa aaataattat tgttcttcaa
    30961 aaagagttag atggctttcc ctttgccaca aaggctcatt taaatattgg tttaagtgtt
    31021 ttgcctgtaa ttgaaacatc tggtttaaat tttctttagt tgagctaccc atatgtggtg
    31081 ataagacgac gttatcaagt tgccttaaag cgagaggaac ctccggttca tactggtata
    31141 catccaaagc ggcaccagcg attgtatgtc gttgcaaagc atcaatcaga gcgttttgat
    31201 ccactatact gcctcgcgca acattaatca aatatccctc tgtccctaaa ttatctaaaa
    31261 cctgtttgtt aattaaatgt tgtgtatcaa taccaccgga acaacaaatg attaaaaaat
    31321 cactaataga tgctaaattt gcggctgttg gacaatacaa gtaagggcta tttttttgac
    31381 ttcgggccgt ataagcaatt tttagcccga aaggctctgc aaattgcgct atcttttcac
    31441 cgatttgacc gaatccaacc aaacccaatt gctttcccaa taaatggttg cccagaaaac
    31501 ggggcttttt ctcaacccat tcattatttc gggtatagcg atcatttaga atcacccttc
    31561 gcgacagtgc taacaataaa gcgatagcaa gttctgctgt atcatgaaca cctgcatttg
    31621 gttgtgaatg cagaataata tgattttgct ttaaaaaatt aatatcaata ttgtccgttc
    31681 ctattcccag gtgactaata attttcaagt taggaaattg cattaaaaac gaatggtcaa
    31741 tctgatccca taccgttgtg gctaaagcca caacccggga ttgatccact cctgaagcca
    31801 ttttccaacc atgaataatg ttccaatcag gaatcagcat gggaataatt ggctctagaa
    31861 attggttggt caggtaaaca aatggcttca ttaaaatgtc ctatttttca gaataaagat
    31921 tccgtgtgtt gtccatgata ttctttctca tctttctgac aagcaaataa aaacaagcgg
    31981 taaaatgagc attctaaagt agagacatat agcgattatt acggttatga tactatattc
    32041 ttttactatt gcatttcatt attccaagga gacgttacca tagagaggaa tgcacagcaa
    32101 tattttgaaa gcgcgaagga attgttcttg ccagtaatac ctgtggagga gtttgatggt
    32161 tttatactta aattgggcaa gaataactac catttttgca aaaatgaaac tccttttaat
    32221 aatagctgta gtgccaatat tgctattgat aaatattgta cctctaaact attggaaaaa
    32281 gctggtattc ctgttcccaa agctatctct ttaaactgta gtgaatttca acggaatttg
    32341 cataaagata aaattgcaca attaaacttt cccttagtca ttaaaccaat agacggttcg
    32401 ctgggaatcg gcgttttatg caacataaaa acatgggaag aactggaaag ccatttaaca
    32461 aaatattttt cctcttatca gtgcttaata atagaagaat tccacggcaa actgaattct
    32521 taccgtgtat tggtttttaa taaccgtatc cttggtatag ttcaacgtta tccagccgct
    32581 gtaactggcg atagagtaca tcccatacat gagttaatta aacaggaaaa tttaaacaga
    32641 aaaagaatca atgaagcttt aggtgaaatt actttagatg aggaagctcg gattaaactt
    32701 caagagctgg gcataacaga aaattacatc ccctcttttg gagaacaaat tgttttatgc
    32761 tatacaagca atgcaacaag aggtgggtcc tatgtcacca taaacaagaa gatgtgcaag
    32821 caaaatcgca aattattgct tcaagccgcc aaaatactcg acttgcaatt agttgggata
    32881 gatgtggaat gctcagatat tatcaatgtt cctatagagc aatcaaaagg ggtcatactg
    32941 gaagttaacc acaaacccag catccgaatt catgagtttc caatgaaagg aaccccacaa
    33001 aaagtcacaa aaaaaattat gcgtagtttc atcacccgcc atccgttgtc ttacttatat
    33061 tctatctaca acaaccaacc gaccgctttt tatgtccgca gctctattct tattatcatt
    33121 atgggattaa ttcttttagc cgtgtggtaa ttcacctcac ctaaaactta tgtcatttcc
    33181 caatctttac tttcaatacc tggcagagag cttatttgat aataaattta tttctgaaat
    33241 cactttgctc aggtaaatgt agttcacttc atgataaatt ctgataattc actaacctcg
    33301 atgaaagaaa tttgagatta aagtacttaa tcgaacaatc tattgagcag gctcattttg
    33361 caagtgctct tttaaagttt atacttaata ataatgaggt taaattatat tacgaggtat
    33421 taaatgaatc ctgtatcgat tttagatgcc tcattaagag atggtggaca tagaactaat
    33481 tttcatttta aggatgatga tttaaaacaa atactacttc ctcttgataa ttcgggtatt
    33541 gaatacattg aaattggcta ccgtaatggt tcacttcacc ctattgaaaa tatagggaaa
    33601 gcaggaatgt gcccccaaga ttatctcctg tattgccaat ctctaataaa aaaagcaaaa
    33661 attgcagtga tggttcatcc tcataatgta aacgaacagg atctgctcga gctaaaaaat
    33721 tgcggtgtga aattaataag aatatgtata gcaaaaggtg atcttgtaaa agctgtccct
    33781 ctgattaaac tggctaagaa tttgaatttt gaaacctcag tgaacattat ccatatttct
    33841 tattatacgg aaagcgaact ggatagtttg attgaggaag taagcaaaca cgaaccggat
    33901 atgatctatt tcgctgattc taatggaagc ttatttcctg acaggataaa ttgcctgtat
    33961 aaaaaataca cccgtcaata taaagtttct tttggatttc atgctcatga caaccttgga
    34021 ttggcgcaag ccaacgctct tgcggcgata aacgccgggg ctcaatttat tgatgcctca
    34081 ctggcaggaa tgggcaaggg aacaggtaat ctaaaaactg aattttttat cgcttattta
    34141 catgcgaaca atataaaaaa atataatttg gaaaatgtgc tgacagcagc taattatgta
    34201 agagatgcgt tgaaaatagg acaagaaccc attgaaatga gtgaattcat aaggggaatt
    34261 tctgatctgt ctacagcaga cctgaaaagc tataaaccag gcaagctcta gaaaatacgt
    34321 acatttccaa tataggaata taaggctggg acaccttgat tccccaagat ttgatgctcc
    34381 agaaagcaac gtactttcaa aattaagctg gcaaaatcat ggatcttcca ggaataagta
    34441 gagaatataa tggcaaaaaa aagattattg attatcggcc ctgtttcaac tcaatcctct
    34501 agtctgaggc acttaagcca aaatttatct tttcttaatc cttattactc cattgagtgt
    34561 cttgatcctc ttgtcacgct gcaagaatgt cccgatgagg aatattatgg aatttggcaa
    34621 aatcaactaa agccactcat caaaatttat gatgcatttg ttggcttttc ttttggtggt
    34681 gttattcttc aacaatgttt ttccatattg agcgaggaaa aaaaacaaat cattcttttt
    34741 tccacaccaa catttgcaga ttccatctta agagaaaaac ttgaaacagt catctccctt
    34801 tgttctcaaa ataagttgga agaaggattg tcttcgctct atcaagcagt ttacgcgcct
    34861 cattccattc ctgatgcttc actgcagtat ttaaataggg aacttgcgat caagaggctt
    34921 atttttggct taaccaaagt attgaaaaca aattgtgttt cgattttaaa tcattgtaat
    34981 gtgaatcacc tgcatttaat aggtgaaaaa tcacaactgg ttaataccaa taacgtgata
    35041 aaaccatcat gtggccaatt gattgtagtt cccgaagcaa gtatgcgagt tctggaaagc
    35101 aaccccactt tttgtaaaga agctatatta aaggccctga atcatgaaat ccactgatcc
    35161 tataaaaatt gctgtattac caggtgacgg gattgggatt gaagtgactg aggctacgct
    35221 tcctgttttt gaagtccttg atgtccctgc aatcctgagt tatggcgata ttggctggga
    35281 gttttggaaa aaagaaggaa cagcaatccc ctccaggaca tggcaattga tagcctcctc
    35341 cgataccgtt ttgctaggag cgattaccag caagccccaa cgtgaagcca aacaggaatt
    35401 aagcaaagcg cttagaaaaa gcaaccctta ttacgtatct cctgtcattc aattaaggca
    35461 aggtttggat ctttttgcaa atgttcgccc ctgtttttcc atcgatgacc aatcaaagcc
    35521 gtttaatttt tgcatcatta gggaaaatag cgaaggattg tattgcggat ttgattattt
    35581 tccattacca aaagcaatac ataatttatt agcagaaagc caacattggc aaacgattcc
    35641 agctgatgaa gcaagttgtg ccttaagatt gcaatcaaaa cccggtttaa cccgtttatt
    35701 tgattttgcc tttaatcatg ctatgcaaac tggcatgcct agagtaactt tggcagataa
    35761 accaaatgtg cttagagaaa gtggcgaatt tgccaggaaa atttttgaaa gcacagccca
    35821 aaaatacccg aaaatccaag cggacatact caatgtcgat gccgtagcat tatggttgat
    35881 aaaaagtccg gaaaaatttg gagtaatcgt tgcggagaat atgtttggtg atattttatc
    35941 cgacgtcgga gcaggagtta tggggggatt aggccttgca cccagtgcca atattggtga
    36001 taaagggagt tactttgagc cagtacatgg tagcggacca agaatgaaaa aaaattgtgc
    36061 caatccatcg gctatgtttt taacaattag catgctgcta aatcattttg gttatcctga
    36121 tcgagcaaaa aaaatagtca atgccgtgat gcaagtcata aaggaaaagc gttatattac
    36181 ttatgatctg ggtgggcact ccactacgac cgatatggcg aatgcagtga ttgaacgttg
    36241 tgcgcgatta gaggcttctt gtttatcaac agattttaat cccgtcccta aagaaaatat
    36301 aattgagtca gatgctatgg gcaacttgct tcagcaatta atgcattgta attctgcaga
    36361 aatttctgat gccttggact cttgtggaat cgaaggcgga ttattgaata tcaaaccgtt
    36421 atcacaggga atgaaaatgg ttgggcctgc ttatactatc caatacttac ccagagaaaa
    36481 aaaagaagct gtcttccata atgccgcaaa ctatattgac aaggttccca aacattcggt
    36541 catagtcatt gacaacaatg gtcaaactga ttgcaccgtt tggggtgacc tattaacaca
    36601 taccgcattg agaaacaaca tcatgggcac ggttgtgcat ggggccgtga gagacgtcga
    36661 atcaattcgc tcaacaaact atcctgtgtt ttgcacagga atttatatgt gctctggtaa
    36721 gaatcgcgtt tacaaagcca atgagcaatg ccctttattc attaatggag ttacaataaa
    36781 tcctggcgac attgtctttg ctgatgacaa tggagtgttg gtgatcccaa atgtgcttct
    36841 tcacgaggta gtgaataaag caattgccat aagattaact gaggaacgca ttaaagcagc
    36901 tgtagcatca ggttccactc ttgagcaagc tcgagaagat tattactatg aacaaccctg
    36961 gttaggtatt aataaaaaga gagagcaata gtgtttttgc ctgaaaaact caaattcata
    37021 gacatacact accatgcagc ccctgatctt tatattcgtc gcagaaatgc aattgaagca
    37081 ggaagattgt atcactcttt acgtggtgca gttgttttaa agagtcatat tggcagtact
    37141 ggtattcagg caactattgc ccaagaaatg ggtttgcccg tttttccttc cgttgtgtta
    37201 aatcatatca atggaggaat aaattataga gtagttatcc gtgccttgag tgaatatcaa
    37261 cctctttttt cgggtaaaat gatcgttgat ttcccaacca tcaccggtcg taaactgcaa
    37321 tcaaaactat caagagactt aactcacaat actttaaaac aatatactgg acagaaagaa
    37381 acactgtttg gatcaaaaca acagctacgc aaagaagtga tagatatttt aaaaatggcc
    37441 cgggattacc ccattgtttt aacttcaggt cttgcctcga gagaggaggt ttataggcta
    37501 attgatgcct gcaatcaata taatgttcct tcattattgt taagccaacc gtctaatcct
    37561 ttaaccggtt taaaatttac tgaactcaat gagttgatta aaaatgagtg gttatgggtg
    37621 gaacagaccg ccttaacttt tatccttgag caccaggaca aacaagattt ttcacaagta
    37681 ttaactcata tccctcgagt catttacagc tcggatttag ggcagaccag ccaaatggat
    37741 attccagagt ggctgcattt ctcaaaaaga atatttcatg aattggattt atctgaaaaa
    37801 cgaaaagagg aactgattct ctccaatgcc atcgcattac tcaacttata aaattccaat
    37861 agacctcttg catataaccc gcctctttca ttctattgct gcagttctca tttactgcat
    37921 gtaaaccgca ctcttcaaaa cgtttgcttc ttgccctaaa acaaaataat cagctgatgt
    37981 aaatcatcag gacttacgat aaacccaagg ttgaggcaaa aaaggttttt aatgagggag
    38041 tttagataaa ctaaatgacc gaattaaaaa ccttttttaa caaagagatt ggggtttagc
    38101 gtaagccctg atcatgcaaa taagtcttgc aaaaacaatt gagtactttg ccaggaacgt
    38161 tgatccgcta atttattgta atgtagtccc atttcatcat cattcgcatt agggtcagta
    38221 aacgaatgag ctgtttggcc ataaacatga acttgccagt ccactttcct ttctgtcatt
    38281 tcaatagaga attgattaac ttgctcgatt ggtactaaag ggtcatcata accatgcaat
    38341 atcaatactt tcgcatgcaa tggcttatta ctctcaccct ccaaaggcaa taatacccca
    38401 tgaaaactga caacccctct aagcggtgcc ccggaacgta tcaaatccaa cacgcacaac
    38461 ccaccgaaac aatagccaat agcagccacg ttatctctat ctacttcagg caactgaacc
    38521 actttgctga aggccgcatt aatacgagtg attaactttc ctctattttc tctgaaaggg
    38581 gtcattaaag ctcttttctc taccttatcc ttacctagct tggcatttcc atacatgtct
    38641 atagcgaatc caatataccc catgccggca agctgaatcg ctttattaca aactccctct
    38701 ccacgccccc cccaatcatg agcaaccatg acacaaggca atggttttga gatactggca
    38761 tgataggcaa taaaaccttt gcacacagta tcaccatcct cgtactccac ttccttagtc
    38821 actaacataa taatcctcgt ttttaaaagg ccgcccaaac cgcattaata tctaactgtc
    38881 aaattattaa aaaatgaact gcacataaat aatgagaaag gctcctgtga gcccttctta
    38941 tagtattgtc ttagaatgtt attcgggagg tggtgtacta gttggagcac cgctgtctga
    39001 accacccagt tgttgacctg tatcgggttt agagcttggg ccagtttggc tgccactgtt
    39061 tttacctttt cctgaaacat cacccgcatt tcctgcaatc tctttagctt ggggtccaaa
    39121 tttgcctagc atttcttggc ctttagctcc caatttgtcg ccaatagctt tctcagctcc
    39181 ataagtagct tcgcctgctt tctctacttt acccttggct tcttcacccc attgcatggt
    39241 ttcctgacca aaagattcgg ggttgccgcc tatccatctc aacactttat ctggcaagtg
    39301 agcaatcagc gtgaaggctt tttgcacgat aattaaatac atggtggtat aaattaagat
    39361 ggagaagaag aaagcataca ctcctgccca ccccgaataa ccgccctgca atgcagttga
    39421 ctgataatca ccgctcaatt tggatccaaa atcatcccat tgcgatgtgt ctattttcga
    39481 ccctgtccaa ttcgcgaccg tctgccctgc ctgtatcatt ttacagtcag caggatacat
    39541 ttgacagtat gcagaattag catttttgtc tgcgaaatca ccttttttcc atgtttctat
    39601 tccttgatcc gattggatgt aggaaatggc atgatcaaat ccggcattca aaatccagac
    39661 tccgacatag gacaaggcaa ttgctgtaat atatcctatt atcatcagag aaggtcttaa
    39721 aaagacatta accaaaatca taatggcaaa ttcgccttta ccaaaagcct cattaccttc
    39781 cggatgcgta acacccaaag cgacaattgg ggcagcaacc atagcctcga taacggctat
    39841 cagccaggca aatgatccaa aagtaaaaat catataaggt aaaacgggta tataatatgc
    39901 tgtaacaaat cctatggata ccatggttcc tatccatgcc attaacaggg gcatagccat
    39961 cataatcaag gcaaagataa agataccaaa aagtgggatt agtgaactga ccacggccat
    40021 attcaacagt gttaaccaca aagtaccaga aaaattaata tacatcgtcc ccatattagc
    40081 caacgcgact atggggttta ctccaggttg cgcaagagtt tgaactccca ctatgaaaat
    40141 atctttcatg ccttgtaaag gaatcattaa aaaggccatg actatggaat taatcatttc
    40201 cccgaatata gctagaaaga aattgtacac gtacctgaaa acatagttat aaaataaatc
    40261 acccatcatg cggccaaggc aaaaagagaa gaacaaaatc ttcaccctgc cgcaatcaaa
    40321 atcctggtgc ttcatgtaat aaagcgaggt atctacttta aaattaataa gattggcaaa
    40381 ggttaacggt tttattcccg gctggcctgg caattggacc atcatggaat tgttgataaa
    40441 cccatagact gttgaagaca atggtccttc aacataagca cgtttagggt caggacttag
    40501 atcaggttgc ttgaccccat cctgactcaa ggccggtgcc ccattgatta atgactggac
    40561 ttgaaccagc ttatcgctct tgttttgaaa ccagttgcac aataaagcat acgtgccctg
    40621 acacgttttc ccaaatggct tggtgagttg tgctggatca aaattactat tatcaaggcc
    40681 tgtgtcacta tcaaaatcac tctcatcttt tactgcactt ccattgagtt tcaccaaatc
    40741 aaaaaaataa gaaccagcca tgatccaccc tttggtattg gcttcttgga taaaatcacg
    40801 ggatttgtca tcatattcag cagatttaga ttgttttatc agattaatgg ttggcgtcat
    40861 aatgccattg tagtcattaa tcgcccctaa aaattcagta ccattaaata atacccccgg
    40921 agtagatccg gtagaactgg gtatcgatcc ccatgtttgg caaacttgtt gatactcagt
    40981 gcagacctga ccagatgcct tataaggcac tccaaactgc tgtttggcaa tggctgaaaa
    41041 atcatttttt gagtttccgg tagacgaagt tgatgaaaag gccggatcat tattaaccat
    41101 cacctgtgcc accgtagata aagtcacata catttgctgt atagcaatcg cgcgagacaa
    41161 ttgagctgtt tccaactggg aagaagtaag ctttatgttt tttcccatat cagccttcaa
    41221 atcagaaata ctattccatg ttactgtgcc gcaaatccca tttaagaaat aaaatggtga
    41281 gcttttatca aagtttggca tatcaaggct aaatggtcct ttagacttgt catcgctttg
    41341 cttcttgaca aaattcactg tactgatgaa gtcaggaatc gctgtacggc aaaaagtatt
    41401 catttcagga gtaggattac ccccacaagg agggctgtta ctttggctca gataaagatt
    41461 cctttgcgtt tgtaattgtt tttgtaaacc caacatgcag acttgaccag acaatatagt
    41521 aaatgcccct tttgctatac ctgtagactt cccaccttta tctgtgaggc tcttggttgg
    41581 gtcagactga gcctgtatta tcacaccacc ccgattcaga taactcaagg cagcttccca
    41641 tattttatca gctgcaccaa caccctgcac aataacccac ataaaaaata cctgcatcat
    41701 acaataacca gatgctttag gtattaacaa agccaaaccg aaggtggaac gaagaggaat
    41761 ccaaattgag gaccattttt ggcctagcat ttgcccttca tgggctgtgt tcatggtgga
    41821 aaccatgagg gtatacataa tgatgattcc acccaaagca aggacagcgg agttgaagac
    41881 accaaacatg ttgcccatta tctggcttcc cgttccgtgc agtaccccat cgactacacc
    41941 aaacaggtta cctaaaaaaa cgacggacaa atcgcttgct ggaggcgcga agcttaaggc
    42001 gccactttca gcgagagcaa gcgcaggaaa aaagcaaagg aggaccgtaa tagctaattt
    42061 attcattctt tctcacccaa taacccttgc ctgtaccatt ctttaacggt acatcccaat
    42121 ttacgttgtt taatctggta gtaccaaaaa tgataacgaa aggctagtac caatgcgatc
    42181 ataaccacaa ccaaacttat tattgtagct ttccatgaac cataaaaaag ttgataacct
    42241 gtataaatga gtaacattaa agcagcaata accataatca cacttagccg gaacaaggct
    42301 ttttgtttga ttatcaagtc agcctcattc aattttagtt ttttgactgc ttcatcaaat
    42361 gattccacat gggttggctc ctgtgggacg aataaacgct taacgccatt gaccaaatat
    42421 aaggtgaatg acttcattct gtcccaatcg acccatttac gaacattgat aatccttgta
    42481 ataactctta cgatccttga tcccgatttc tttttcatat tttttacttt ttcacaattt
    42541 taagtaatgg acttttatat atcataatat actatagtat taagtagttg tttcgtctat
    42601 caacgtatct cagtaaaaaa agagtaaata atgcctgatt taagccatga agcctccgca
    42661 aagtactggt ttgagtacct tgaccccatg atttacaggg tcatcacttt tatggaaagt
    42721 gtggaaaact ggactcttga tggaaatccg gaactggaag aggcaatgaa acagttaggg
    42781 caagaacttg atgatattga aaaaattgac ttgggcttgc tagcagaaga agacaaattt
    42841 attcgtatcg ttggaaacat caaatcagga agaggcttac gcttgcttca ggcaatagat
    42901 acagtccatc ctggtagcgc ctcccgtgtt ttaatccatg cggaagaaac cagtttaagc
    42961 agcaatgatc cggcaggttt ttttctaaaa cgcaatattg tatttgaaag actaaggtta
    43021 ttatcgcgag ttttttgcca ataccgtcta aaactcgttt tacgagcact cgaaggggat
    43081 gaataatgaa agtattacct aaactggcct tggccaatct gttttgtttt accactttat
    43141 ctgtatctgt ttgcgctcaa tcgggtattg atacgtcagg acaacaaaca agcaccaaca
    43201 cgtccaatct ggtgacttac ctcaccaatc tgggaaaata tttaggctat gacatcactc
    43261 aatctaataa aacgcctaac ccgccttatt cacaattatt taattccaat gtagcccaac
    43321 tcattcaaaa ttacgcttac aatacctttt tgggcgctat ccccgtgaat gccatgtccc
    43381 agagttttat gaattttgtg acccaaaatg tgcaaggaag ctcattaatt aataatttgg
    43441 cgaataatac ctttaaatat caaaatttca gcaccccatc atccggcctg aatggaacag
    43501 tttctgccaa cagtttaata gaccaatcaa cggccaatgc ccaagcccct accccctcag
    43561 gagttttttc agacccacaa actacaccct ccggaactta tttgaaggat ccagtcagtc
    43621 aggccgtatt caatattctg ggaaccccag attacagttt ttgtatggac aacgagcaaa
    43681 aaaactggtt gccaaattgc aacctgatgt accaaaacct ggtcatgcaa aatgttatcg
    43741 gcactttgcc cccagcgcaa acctctggtt cgacacctgc tttttatacc tacaattata
    43801 accaatcgct cattggccaa ctgaacggca actcgctgat tgccccgttg ctattagata
    43861 ccaatccagc accactacag caattgagta gctctggagg cttaaaagct atcagccaag
    43921 cacagcaagc gcttaatttt attagatacg caagtgctca agtcactcct ccttcattac
    43981 ccaagttgag tgcctacagt gaattatgga atcaagccac tgccaaaccg aattctgacg
    44041 gttataatga agtccagcaa aagcaagcag cagcaacatt gagcggttat tttaataatt
    44101 tgcgcgttta cgcagcacaa acttctgtcg gagtgagtaa tctctactat attttatcca
    44161 aaaggttacc ccaaaatatg agtggcgacc aaagcaacca aaatctgaca agccaggcgt
    44221 tgaatgaatt taacatggca actcgaagat tatttgatcc aaccgcctcc aacactcccg
    44281 gacaaccaaa ccaacaatgg attaaacaaa ttaatgacgc ctcccctgct acagttcaaa
    44341 aagaaatagc agtattgttg gctgaaatta attatcaaat gtatttggac agacaaatac
    44401 aagaacggat attattaact aacagcatta tgctattgca aaacttaaaa gcagcgcaac
    44461 cgaccgcaga cttttcaagt caaggctctc cttccgagca ataatagtca tcactttggc
    44521 tcctgggatt ccaattccag agagccattg taaccaggtt tattaacacg cttaaaaatt
    44581 tggtacacaa aatcaatcat ttttttccct tgtggttggt ttttgtttaa ataaaacccc
    44641 gaatttaatg tgctaactaa ttgatagttt ttaaaaaaat gtttttcata tagcaaatct
    44701 gttaaataat cacctcgtga ttccataggg tagtaattgg tgatcaccca atcaggcttc
    44761 actttagaat tgagataatc ggcataaaga cttaaatcgt ttttgaatgg ctttttggtt
    44821 aaatcgttat tgttcaaaca ttgcgcgtcg atgaatcgaa tgtcttctcg agaaaaaaaa
    44881 ggaataatcc cacaatcatc taacagaacg ctgtcgcctc tttttgcctt tcggttgagc
    44941 aaatcagcaa tagtcattct attttgagtg cgttcattat attctgcaac tgatttggat
    45001 aaatgattta aatcattccc gggcacaaaa aaccaggtaa taaacaacat taatacagtg
    45061 gccagatatt caggatccca tctgaaaaat ttaaaatatt gtatcaattg attaagccct
    45121 aaaacaggta atatggaaaa aagggcaaat gggcttaaaa acaatctcaa accgtatgtg
    45181 actacaggat ttgctttcca aagcaacaaa gtatataaaa cagaaggcaa ccaaagtaaa
    45241 acatggcgac aatctttatt ggcagacaat aaatagggta ccgataatac aagcaacggt
    45301 gttataaaaa gaaaataatc aaaatctact gccaaaaact gcccataagc taacgctttg
    45361 caacgatagc tattaggaat ccaatgaccg aaataaatta atcgccaaat aaaatacaca
    45421 agatagggca atacaaagca aataaaagta gttgaaaccc ataagaacaa tctcttatga
    45481 tctttaaaaa ccagccccct ttgagctctc aaatattgac acccgataaa aacaaccaaa
    45541 ggaattatcc aaacgattcc ctcaaatcgg gttaagctca ataataacaa gagcatattg
    45601 gttattatcc aatgagtggt tgagtaaagg gaaaatcgat tgggccatgg cctgtcacca
    45661 acagtttgat aaccgaatgc caaggtacac tgccatatca atgccaaaga cagggcgcag
    45721 taaaataagg tttccaatcc actgacagcc caccaaacca ctcctgtata atggctaaat
    45781 aaaaaaacag gtaatatgga taacagaggg ctacaaaaaa gtcttgccag tcgatataaa
    45841 aacagtaatg ccaatcccag gctgcaacag gaaatgacct tcatactgct caccagaggt
    45901 aattgcaatt taatgaccaa tgcagcaatc atgagccata aaaagttgga atacccctct
    45961 actggcggca gagtcacatg ccaaaataac cctttgccgc tcactagctg tcgcgcgtaa
    46021 taccagctga tataagcatc atctgttgtg aaatcccaag ctgtatacaa cgcgaccatc
    46081 agatcgtaag cgcaataaat ggctatcaat agcaccagaa tggtttttct tcttgataag
    46141 agggaactca aaatgtgttc agctccagaa aatttttcta taacacaatc cacaatagca
    46201 atatcacaaa gtatcaacaa gctccaaaat catgatgcaa aaaaaataat gggtatgtta
    46261 tgattccaac ccaatttaaa aaaactgtat cctcaatgga ataatacgga acttacaaga
    46321 aacgccaaaa gtctgggtca ttgtgataag ggagtaccaa aataacattt caaaacagtg
    46381 aatcacatcc actggactaa agatgataga ccttgattta aattaaaaat gtaaccccta
    46441 ctcaatggtt ggtgttgatt gataaatccg gtacaaagtt ctcaggatga gttaaccatt
    46501 taactcagcg agcgattatg tccaaacctt attattttta tgtcagcgga atgaattgca
    46561 ccagttgcag taatgccata gagtcctttt taagaaattc caatttaatc aaaatcaata
    46621 catttcacgc ggacatgagc accccggatc caaaaaaaat tgtgatatct ctttctgaag
    46681 aaaaggaaaa tgaacatgaa agcaactgga aaaccattaa aaaattaatt gatgatacag
    46741 gttttacttg cgaaaattat gaattccctc aggtgagaga tcaaaaacct gctcttcctc
    46801 tcgagaaaac gcagacaacc gttcaacagg aagccagcca attatccgca ctctttagca
    46861 gcgcaaaaaa aataatcacc tcccattggt tgcttggagg attgggttgt atcactgggg
    46921 ttagcttaat gatcctgact atggctctag gtggtttacc acttgcggta atgatccctt
    46981 tagcaagcat cagcacttta ttaactattg cgcttggtgc ccaatcttat tacgaggctt
    47041 ggaaaaaatt agtccaatct cgcacactca caatggacac attattttca attagcacta
    47101 tttctgttct tgttgtgtcg cttgcttcat tctttgtacc atggttgcct atgatgtttg
    47161 aggcaggatt acttatttat ggattcaggc atattggtat cgctattgag gacactatca
    47221 aagacaaaat aagctccgcc aaatttcaag acagagcccc gcaaaaagtc agattatatc
    47281 gccatcctga ctttaaaaaa acgaggattg atgagattca acccaacgac attatacaag
    47341 tcaaaccggg tgagcttata ccattggata gcgtttgtga gcaagacacc ctcatttatc
    47401 ccaccatcat taccggtgct gtcataccta aacaatttcg tagtggtgaa aaaatacttg
    47461 ccggaatgcg tctggcaaat gatgcaaaac cactcagcct ccgcgttcta aaaactgcaa
    47521 aagagtcata ccttgcccgt cttgatgaaa gcatagctca atctgtactt gaaaaagcac
    47581 cgatagaggt aaaaacaggg cagcttctta gctattttat cccatccatt attgctctct
    47641 ccatcctttc agggatcatt gtcggcttga ttgccacacc agcacttgcg atacaatgcg
    47701 ctatttcagt attggtttct gcttgtcctt gtacgcttgg tttaattatt cctctagcag
    47761 taaaaactgg aattcataaa ggagccgaac acggtgtgca gtttaaaaat tccagggtat
    47821 tacaattagc agaacaaatt gatgtagtca tttttgactt gaatggtacg ttaacaaccg
    47881 gcgttcctgt ggttaaagaa tatgctgtat taaatgatgc tgatctttct tctgagcaat
    47941 ttctgagtat ttgcaatgtg ttggaagaac aatcaaaaca ccctttcggc aaagcaatac
    48001 aatcgtttac ccaaggaatc aataaacaaa aattcaatat tgctaatttg gatcattcga
    48061 accattcagg cgtttcagca gagattaatg gtattcaata tactattggc agtaaaacct
    48121 taatgcagga aatagggatt tctacatttt cgcttgaaaa ccaaatgaat ctggttgcgg
    48181 gcgatagcct agtgtttcta gccagaaaca aacaactatt aggttatctg gtcatcaccg
    48241 accccttacg aaaagatgca aagcaaacag ttgatgcttt aattaaaatg ggaaaagaag
    48301 tgcatctatg cactggtgca gatgaaaaaa ccgcctatcg ctatgcgcaa gctcttggca
    48361 ttaagaaggt acatgctaat tgtgtcgcta caactatcaa tacagaggat agatccaaac
    48421 cggtttatat taaatcatta caaaatcagg gactaaaagt agctatgatt ggagatgcgg
    48481 ctaatgacgc aaacgccctt gctgctagtg acttcggtat agctgtactg tctcaagaca
    48541 gcgatgaaat cacccagcaa cacgcctcag ccattataca aagtggaact atattgccta
    48601 tagccaatgc ctttgccata tcaaaacaaa cagtaaacaa tatcaaccag aatttgttaa
    48661 ttagcttagg gtacaatttg acaacagtat taatagccgg cggtctgcta gtcgcccttg
    48721 gttttgtgct aaaccctgct gttggtgtgg cattaatggt aacccaggct tgttttgtct
    48781 tgctcaatgt ataccgcttt aaagagcaac ccttgcaaca tttgcaagac aaagttcagg
    48841 aaaaaccaga accggctcct tcttcttatg aggccatgaa aaagcattca cccaatttcc
    48901 atacagctcc cgagattaat cattcgcaag ccattcaatc tggaaagcaa ccaaccaata
    48961 aaaataaatc accttccttt tggagtgatt attttcctga attatttgag gaagaaaagg
    49021 acacaagtac cgaacaccta atgaataaca aaataggata agccctaaat aaagtttggc
    49081 tgaatttgaa atgaggaagg ctaatcacct tcctcaatca taacaatcta ttttgattta
    49141 ttatgctcta aagggtcaag aatgtgatgt ctcttaccaa tatatgatga ctcataaaaa
    49201 tcgcatgtga aatgaggtaa tcagcaatgt ataccgcatc attatatgga caataaataa
    49261 attatatggt caaattggca aaattaatta acttacattt taagaaaact catgccaaca
    49321 acctttgaag ataaatcatc caaagtcccc agtttaaaat ccctggccgc taatgccgtt
    49381 caaaaaacca atgcaaatct ctttttcagg ttgcactcac ttgaaactcc ccctgagata
    49441 aaaaaagggt ttattgacaa ggagctagag gcattaaccc acgaattaac tgaagactat
    49501 caaaccaagg tggaagccaa gaatgaaaaa attgaggaat gcagttccaa tcttagttct
    49561 aatgaatgtt ttgtaaaatg ctctgctttt accctcacta cattgatggc tggagtgcac
    49621 gttggtattt attacatcct gaaagcagca gctgtcgacc catcaacgca aatagcatat
    49681 atttcgagta ttcccgctac tatttgtttc tctatgtgcg ttggagtctg cttgaatcga
    49741 caaattacta aatgcttgag ctcttgtttt acgcccagtg tccccgataa aattactgtt
    49801 gatttggatg agttgggtag acaatcccat gttagtccat aagcggtttt ctgggttcct
    49861 tgaaagaaaa aaccgactta ttcgctaaaa tataatttaa ttacaatgat tcttcactcc
    49921 ctggtaattg ttaaaaaata gggagtggtt tcatacaccc attttccacc gtattttttt
    49981 ttgcagtact catcaaaaaa ctgcaccatg ctgtttacaa tatcttgata cattttaccg
    50041 gggagatcct tgaataatgg ggctccgtgt ataaagtttt caaataaccc gagatcaggt
    50101 aattccactt ggcaatgttc tacaacaaaa gaaatgcttt taaatggcaa attatccaat
    50161 gcccttcggc attgaataga ataattctcc cgcatcattt tagattgcac gacattttta
    50221 gcaacatcag ggtaaagatt ttgaaattgc aggctgttta acacttcaaa caaagtgctt
    50281 tgtttgccag aagaaaaaat tgcacaaatt ttacctcctg gtttcaagac atgatataaa
    50341 ttggataatg atttttccaa atcagtccag tgaagacacc aaaatgataa aatattatca
    50401 aatggcccgt aaattatagg ttcttgaata tcccctatat caaaacgaac gttgatactc
    50461 ctgcaatgtt tatttgcata atcaatcatt gtttttgatc gatccaagcc aagaacatat
    50521 ccatcaggga ctaaattggc tagcatttga gtgaaactgc catcaccaca accagcatcg
    50581 agtaagtaat cattactatg gatagatatc tttgcaagaa acttgctgga gatttctctt
    50641 aggatatagt taccttttgc ataattagtt gcagaccaat tattcaatat agcaagcaaa
    50701 ctttcattat ccattcggga tttcttccaa aatgatttac caagagcctc ttgtaaatta
    50761 tagagttctc caacacaatt ttctaaaaat tggaaacttt tgatgttgta ttctctgaga
    50821 tctctacact agtgatgtta taaacaagga atcccattag gccattgcaa ccaatttttc
    50881 tcaggataag gtaatgaaat atcaaccaaa tgcaaagtat aggcatgtct ggattgatta
    50941 gaaaaattag cctggctttt atgtggaact ctgccatgta gtataattaa agcgcctttt
    51001 ttaacaggta aagggacact gttttcttct ggccaaggac ttgaatcgaa attttcaaaa
    51061 taaatttcgt tttctcgacg aaacatcctc tgctttaagg gtgtggtaca agggcttgga
    51121 atcacttcca aacagccatt ttccaaagta gcatcttcta ttgcaaacca aaaccctaaa
    51181 gcatcactgt cctctccaaa aatataggta ctatcctgat ggcaaagtac ttcggctcct
    51241 attcccggct gtttgaatat gtacatcgat tgtaccaatc caagtgtatt caacccaagc
    51301 tgatgtgcta tcttcttaat tcgatcatct cgggaatatt gcctaaaaac aggatccagt
    51361 tcatgtaaag cgtgtccaat tttattaatt gattgattaa aagggcgtag acaatcaccg
    51421 gcttcatcaa aagcacccgg ctcaaaaaaa taatgaattt tatctccaga atcaaggaaa
    51481 tattgttttt tagcatgctc gttagtttgc gtggaaaaaa ttgttttagg tatctctgcc
    51541 tgattatttt caagcaatgt ttcaatgcgt tgctttaata gatcgcatac agattctgaa
    51601 aagaaattat ttattaccaa atatccattt gtgaaaaaaa aatcacattg ctcagaagaa
    51661 agttgcatcc tatgctcctc aaaaatcaaa atcagtactt ggggaaatca ccagggtaag
    51721 ggtaaacggc ttttaatgag cataagcgaa atgctctatt aaaaaccttt cccacacaaa
    51781 gattaggtat tgttcataag tcctgaaaat atttcagtta atttttccat tacatcatct
    51841 acggtaatta atttcatagc ttcatatcca tgaacctgcg taccccaaac actcttctct
    51901 ggactcactc ctaatacagc ttggatagct tgcgaatacc tgttaacaac caaatgttga
    51961 aaagtatacg ggccagaaat agagggattg gtaacagcat gcaaagccac aacaggcgtg
    52021 cctaccgctg ccgccatatg agaaggtcct gtatcagggc acaagactgc ttttgcttta
    52081 ctgatcacag ccaacaattg cttaggtttt gtctttccaa ctaaattaac cactggaatt
    52141 tctttcgcta tttcctcagc caatgattta tcatgatcac ctggccctcc agttaaaaca
    52201 actttgacgt cccatttaac ttgcgcctgt ttcagaacct caatatatcg ctccgccaac
    52261 caactgcgct caggtttgct ggctgcggga ttaacgacta aaattggcct ttcttgttcc
    52321 ggtaaatgat tttgagccca ttcataatct gccttggtaa tcggtaaatc ccagtggatt
    52381 attttttcag tgacacctaa aggctcagca aattttaaaa agccctcaag tgtgtgctcc
    52441 aatccaggag tgatgctttc ctttataaac agacgatggc catcattcgc tctgtgagaa
    52501 tcatagccaa ctttgcgttt agctttaata aatggaataa gccaattagt tctaaaactg
    52561 gcttgaggcg ctaacaacac atcaaactgg cgaggcaaca tttgtttcct aaaccgccaa
    52621 tagtcagcaa gcgttttagg tttatcaata acaataaact ccacgccttc cattccttcc
    52681 accaaataaa aggcaggttt tgaaataatc caggtcaatt gagcattagg caatttattt
    52741 tgcaaggtcc ttatcagtgg gaccaacatc aaaacatccc ctaaagctga aagccttata
    52801 acacaaatcg atttaatcat tctgcaatac aaaacgagag ccacaataag ggcaaacctc
    52861 caccccggtt ttctctatag gcaaataaac cttgggatga gcattccata actccatctc
    52921 atctgttggg caactcaatg gtaattgatg tctatgtacg acatagtttt ttttagcact
    52981 tgcaggttct tttgttgttt tcctcatgtt gttcctttgc ggcatgatgt caattagaga
    53041 cagtattatc taatatttcc cttattttcg ctataatatc tatattaaaa cccacatttt
    53101 gcgaatcata aaataaagcg ccctcccaat gcctcaacca ggttaattgc cgtttggcca
    53161 gctgccttgt tgctgctata cctttttccc tcatagtctc ataatcataa tggcctgcaa
    53221 gatactcgag tatttgccta tatccaacac atcgcatgga gggtaagttg atttgaatag
    53281 gccattttgc ttgcaattgt tgaacttctt ctatgaatcc ctcactaagc atcgcgtcaa
    53341 accgttgcgc gatacgttca tgcaaccagg atctttgctc aggaaaaaga gcaagattaa
    53401 caaaataata atcaggctgt acatggggtc cggtcaaata ggttgataac gttgagccgg
    53461 ttaagtaata aacttccagc gctctttgaa ttctttgcgt atcatgagca tgaatgcgag
    53521 cggctgttac cggatctaat tgacttagct tttgataaag aaaatcccaa ccctgtgata
    53581 atgcttcttc ctccagccgt ttacgaacag cttcatcggc ttcaggcaaa gtggctaacc
    53641 ctttttgcag agcattaaaa tacatcatag ttccgcccac taacaaagga atattccctc
    53701 ttttgatgat ttcagcacat aaagacaaag cgtctgtaca aaactgggct gccgaatagg
    53761 attcaaccgg atctttaata tcaattaaat aatgaggcgc tctctgtaat tcatggattg
    53821 aaggttttgc tgtcccaata ttcatatctc tataaatcat ggcggaatca acactaataa
    53881 tttcaaaagg gaaatgagtt agcaactcgc atgctaaccc tgtttttcct gaagcagttg
    53941 gtcccattaa acaaaacact agcttattca tgcaataaca tccgacaatc atcaatggtt
    54001 aacgctttac atatattatg tttctcttct ttaagagaga gttccagcaa caattgattc
    54061 atttcaatac gctcttcagc tgttaattgt tttgggtcaa acatttgaga acggctaatt
    54121 aacgctatca attgctcagt atgaaaaata ttcaattcac agagaacatt aaaaaaataa
    54181 cgaacatcta aatagggcgc attcaaaggg atgctgcgaa ttaacaagtc atcagcatta
    54241 gggtactcaa tttgtatccc aacacgagcc aactcatgct ttaattgctc ctgcttatgt
    54301 atccattggt ttggtaatgg gtaacgaatt gaaatcaata aaggcctgct ctcaagaggt
    54361 aatgcctttt gcattagctg attttctatc cattcttgaa acagcgttac gatgtctgca
    54421 aggtaaggct gttgctttat aaaaaccaaa ataaaccgct catttaaaat aacccactga
    54481 gactctgttt ctggatgata gacagagctg gtttttttat accctttgtt ggttatagat
    54541 gatgataata agttggcact ccagcttggg gataaagctc ttgccccttc atgggactct
    54601 ctgtcattgc agatatggga aggttccgaa actgtatttt gctgctcatt acaagaggaa
    54661 gcagcatacg catattccct tgtatctgaa tggtcaagag tcgattgcaa cgctttcgtc
    54721 aattgggaag taaaaaaatc atgtaccagg cggggctgtt ggaatctcac ctcatgcttt
    54781 gtaggatgca cattcacatc cacttcagaa gcaggtagag taaaatacag caaacaaatt
    54841 ggaaatctgc ctgcatgcaa taagccatca taagcttgtt ttactgcatg ttggagtagt
    54901 ttatccttta ccatgcgttg attcacataa atccactgcc tatcattctg gctgcgttgc
    54961 aaacgatggt tgcttatcca gccataaaga ctcataccac tgtgctccac atcaagaaaa
    55021 atagcttctc tcatgaaagt attgcccaaa attcgaccca tacgaatggc tttggcttgc
    55081 tcacttaacg ctggcggtaa tgaaaaaatc agtttcttat tatgcttaag cgttaatgca
    55141 atccggggag cacttaatgc aaaccgtttc acaaccattt caatagcttg aaattccagt
    55201 ttttcatttt ttaaaaaccg cttacgaact ggggcattat agaacaaatc agaaacatca
    55261 atggtagtgc caatattgcg cgcacaagga gagagagtcc tgttctcccc ttctactcgc
    55321 aacatcattg cattgtcctg ctcctcaggc tttgaaatga tagtcacttt ggcaacagag
    55381 gcaatacttg ccaatgcctc ccctctaaag cccatactat caatggaata taaatcatca
    55441 agcgttctga ttttactggt cgcatgagct gcaacagcta aaggcaagtc gtctccagag
    55501 attcctgaac cattatcact aactgtaatc tgcagcaatc ccccataatt gacatcgaca
    55561 ccaatcacac tggcaccagc atccaaagag ttttccagca gctccttgac taccgaagca
    55621 ggcctttcta taacttctcc tgctgcaatc tgattggcta ttgctggcga aagttgatgt
    55681 atccttaaga ccatgtgcca ggtatcacta atttttgccc gggcataagc cttgcccttg
    55741 cctgaaggcg gttaattgat tgtaatgcgc tcacagatac cctatatcga gcagcgatag
    55801 caggaagagt ttcacccttt ttaacgatat gaaaatttgg agaaagcata gcctcaattc
    55861 gagtcccatg aggaggatga tcccaaaaat aattttttag tccttgaaat attgcctgac
    55921 tcaatcgctc ctgataggca gggttggtta aatttctttc ctcacgggga tttgagataa
    55981 aaccggtttc aaccaaaata gaaggtatat ctggagattt cagaacaaca aaacgggctt
    56041 gctccacttt gttattatgc aaattggtaa aattatccaa ttggcctaat acttttcccc
    56101 ccatttccaa accagcattt attgttgcag tttgtgataa atcgatcaaa acagagcgta
    56161 ctaccccatt actatcatcc aactcaccta aattcactcc acctagttca gagtaattct
    56221 ccttttctgc taaccagcgt gctgcttcac tggtggcccc cctttgtgac aaggcaaata
    56281 ccgaggctcc attagagtga ggattattga atgcatcggc atggatggaa atgaatacat
    56341 caccattata ttttctggca atatccaggc gttgtctaag tccaacgtaa taatctcccg
    56401 atcgtgtcag gactgcacgc atacctggtt gcctgtctat taatcgttta agttttaaag
    56461 taatggccag cacaacatcc ttctcgttac tacgacgcgg acctcttgca ccaggatctt
    56521 tgcccccatg ccctgcatct aacactacaa caacatcacg caaaggtttt tttgatgggt
    56581 tatgaggaac ctggacaggt gttttatgca cagttattcg ttgttctgat ggctgactgg
    56641 atatttttga ttggatttgc tggacaggag ctttaatcgt tttaacaggc gagctcaact
    56701 gcctgcgatt atttatgact aaaggagtat cattaacaag atcaattctg atcccattaa
    56761 attctccttt cggtttccat gaagatgagc gcaccttgac tggttgtttt acatccatga
    56821 caagcctgag cgtgtttttg gagggactac cgcttcttac ctgctgaata agcgaattga
    56881 ttaaacccaa ttgactgata ttgacggcta attgtgtatc acttaagtct aacaccactc
    56941 gattgggctg acttaaggta aatagcttat gggtaaatga accttgtata gaaagataga
    57001 gagacgtcat ggttggttgc tgagaaactg taatattctt taattgtgca gaaaatacag
    57061 caaaagaact tggtagatat aataacagac ttaagaccca cgctcgcatc atcattcacc
    57121 cgccaggcaa gctaaaattc ttttacccgc gacacttaat gcagctattt gtattaagcg
    57181 cccagtccct ttaattatta aattaaactg aatatcgact ggaggcaatg tttttccacc
    57241 atgttcaggc cattcaatac aacaaacgct ctgattggaa aaatagtctc taaaacctaa
    57301 atactccagt tcttcttcct ggtgaatgcg gtacaaatca aagtgatgaa tatgaaattg
    57361 accacaattg taactttcaa ctaatgaaaa agttggactc ttgatggctg aaattacccc
    57421 gagtgatttt agcattgcgc gaataatggt ggtctttccg gcccctatat caccacttaa
    57481 agtaatcacc aaaggcgctg agatacaaaa cgccaatttt tctgcaaatc ttttgctctc
    57541 ttgttcacta accaaatcca aagttatgaa attatcatta tcttcaatag ccatatcgag
    57601 aacactacat aatgtatctg tttagaaaaa gcctgtcagc caaaccgtta tgcacaagta
    57661 ttgaaacaga gtttgttacc tcagtaacac taaggttacc ttcttaataa atcatcgcac
    57721 tagatacaat tcaaaatgct tggaatttca tcaagtaagt cgctcgccaa taccccaact
    57781 tctcctgatt tttcagcaac cttgtctcca gcaacagcat gaacccacac ccctaacttc
    57841 gcagcatcgg ataaggtaaa gccctgggcg caaaaagcgg caataatccc actcaaaata
    57901 tcccccatgc ctgcggtagc catacctgga ttgccttgag ggcacacaaa aatattcctt
    57961 tctgctgtct gaataaccgt accaactcct ttcaatacca caacaccacc atattgtttt
    58021 tgtatagcag atgccgcatg gtatctatcc atttgaacat ctcttgcggt acatgaaagc
    58081 aaactggcag cttcgccagg atggggtgtt aaaatccaat tgtcatccat ttggggatgt
    58141 tgagaaagta atctcagcgc tgaagcatca atgatcatgg gtaattgggt tgtaatggcg
    58201 gttaaaaaca aggttttagc ccaatcacta tcaccaagcc caggcccaat aacacaaacc
    58261 gtagcttttg ctaatagagg cattaactcc cctgctgttt tgatacccca gatcatcgct
    58321 tcaggaatta agggtaaagc gctcttcgca taatccggcc aggttgcaac agtaactact
    58381 cctgctcccg tcttgagtgc tgccttggct gccaagctca ctgctcccgg cattcctggg
    58441 cctccgccta tcactaaaac atgtccataa tcacctttat gagagttctt ttttctagct
    58501 gcgagaggca atggcaaggt agaatccctc agcagggaag caaatcgggt taatttggtt
    58561 ctgcatgcct ccaggtttaa atgagcacaa tgaatcacac cacaataatc tggtccgtcc
    58621 aaagtataca ttcctgtttt aagtccgata aaagtaattg tggcagtcgc attgacgcaa
    58681 atattctcta ctaatcctgt atctgcatta agaccagatg gaatatcaat cgataacacc
    58741 ggtaacccac tggcattgat ttgatgaatc gcgttggcta ttaccccatg cactgctcct
    58801 tgtaatccaa tacccaataa tgcatcaaca attaattcgg catcgccatc taaaggttca
    58861 tctgcggatt gacaatgtac tccagatgtc aatgccaggc gtgcggcatt ccgggcagcg
    58921 gaaggtaaat cttctattga tttacattga tatacagtaa ctgagaatcc ttgttcatga
    58981 gctaatctgg ccaaaacata accatcacct gcattattgc cagcaccaca gaagacggct
    59041 atatgccgta catgcgggta gattttctgt aagaaagaaa atgcctctgc gccagccaga
    59101 gccatcaggg cattttcgtc tttttgatac aaagttgcag cttgctgttc acaagcttta
    59161 atttgatcac aacgatacag agcattttct agcttgttca cctctcggct ccaaattaac
    59221 gtaaatttga tataacgacc acaaaatact tttatatcgc tatgccaaga tcatgctgta
    59281 aaaatcagtg cttcttggct acgattctct taaaagaaac catgccgtct actttaccaa
    59341 ttttagtgat ggcttatttt ttgtagcact ttcagaggga gaagaagttt taggtggtgg
    59401 ctcatcatct tcgtcaactg aaaaagcaat tcctctgcca ttttcttttg cataaatctc
    59461 tagtacagca ttgggagcaa caaaaatttg ctctgtttgc ccagaaaagc gtgccgtaaa
    59521 cacaatgcga tcattttcca ataaaagtcc tcgggtcgct gagggagaaa tgtttaatac
    59581 aattctatca tgctgaacat gttcccttgg aacctgcaca ttgggatttg atgcatctac
    59641 gagaatgtag ggtgttaaat cattatcaac aatccaatcg tagaaagcac gaatcaagta
    59701 gggtttgttt gatgtcattg ccataattat gctgctctta tttgtctctc agcgtccgtc
    59761 aaactcgttt gaaaagaatc acgtttaaac acacgctgca tataggaaat tattccttta
    59821 aaattaggcg caatttccaa acctaattta ggtaaccgcc ataataaagg agccaatgca
    59881 caatccaaaa gcgaaaactc atcacttaaa aaataaggtt tatctgcaaa gacaggatct
    59941 aaactggtta aactttcaag taaattagct ctggcttgct ctacagattc ctcccgcaaa
    60001 atagcgttca ttaaataata ccaatcctgt tcaattctat gcatcatttt gcgagcttca
    60061 gcacgagcta ctggataaac tggtaataat ggaggatgag ggaaacgttc gtccaaatat
    60121 tccataatga ttcttgcttc atataataca agttccctat caattaatgt aggaacagtt
    60181 ccataagggt taatagacaa taggtcggct ccaggatcat cttgctttac tgcaagaatt
    60241 tcaacattaa ctcctttttc tgccagaacg atacgaactt ggtgactata aacatcatct
    60301 acatcagaaa ataaagacat tatggtgcgc tttgcaacga ttgccattac cttactccta
    60361 tcaattcatt ttttaagccc gttaaaatgg ctttttatat tctagtgcac aaaggttgct
    60421 attctatcac atttttaaac ttctgggtta ctttgagcaa atcttcttat tttagcgtaa
    60481 agtcgataca agaaaccaga tatattttct gattctgaag gtaaccattt gagattgaac
    60541 tctgtataaa aggcaaaaaa gtagcacctt ttacaaagct gaggagtaag cagtctattc
    60601 caattttaat tgtttccaat aagctttctt cagtgaataa gctgctataa ggaaaataca
    60661 gaagaacaaa agaacaaaaa ctcctaattt ataacgaatt agcttggcag gctcacctac
    60721 ataaaccaaa aaattgacta aatcccttaa tgcgctgtca aactggcctt caaacatttc
    60781 gcctcgtttt accactaata aactatttgc atgcgaattt ttatctttga ccaaaatgac
    60841 ttttccaatc aagggttcca aaacattagg catagcaaca tcaggcatta gtaaattatt
    60901 agcaccaaaa ggtctggaag gatcgctgta aaaacttttc aaataattat atatccaaac
    60961 aggccctcta tcccgcgcac ttaaagacag atcggggggc accataccaa accattgctt
    61021 ggcatcttca ggcgacatgg caatttgaat gggatcataa atattggcct gagtaaaaat
    61081 taagttattt tttaataaat cttcagcaat ctgaccatca aaagtagtta atccaagatc
    61141 ctgtgccatg cggttataac gcatgtattt gagagaatgg catcccgagc aataattcat
    61201 aaacatcttt gcccctcttt gcaaactctc tttatcgttt atatcaaccg gtacataatc
    61261 cattgctaaa ttttctgtaa aagcagcgtt tgatattaaa aatgccaatg cagcaactaa
    61321 taacaccttt attttcatca tttttcacct atccgtgtag gtactggccg ggttttctca
    61381 aaacgactat aaataggcat caagataaaa taagaaaaat aaataacagc ggcaattcga
    61441 gctaaaagca acctcactgg tgtcaccgga gttgtgccca aatatcctaa aagcagaaaa
    61501 ctaaacgcaa acaaggtaat acttattctg gaatacattc ctttatatct tacagagcgc
    61561 acattacttt tatcgagcca gggcaaaaag aaaagcaata atatggcagc agccatgcac
    61621 accaccccaa ttagcttatc tggaatagcc cttaaaatgg cataaaaagg tgcgagatac
    61681 caaacaggag caatatgatc tggagtcacc attgggttag cgggtacaaa attagcatgc
    61741 tctaaaaaat atcctcccat ttcaggggca aaaaagacaa ccgcaaaaaa taaaattgaa
    61801 aagactatga tacctacaaa atctttcaca gtgtaataag gatggaaggg tatcccatcc
    61861 aaaggctttc catccttatc cagggccttc ttgatatcaa tcccttccgg attgtttgag
    61921 cctactttgt gcaatgcaac aatatgcaaa aacacgagga tgatcattaa aataggtatg
    61981 cctatgacat gcagggcaaa aaaccgctgc aacgtcggat ttgcaacaga gtaatctccc
    62041 ctcaaccata cagcaagacc tttgccaata taaggtatcg ctccaaataa ggaagtaatc
    62101 acttcagcac cccagtaaga catttgtccc cagggcaata aataaccaaa gaatgcttct
    62161 gccattaaca agaggtataa aaacattccc agtaaccaaa ctaattcccg tggtttctga
    62221 taagaaccat aaagcaagcc tctaaacata tgcaaataga taacaataaa aaaagcagag
    62281 gctcctgtgg aatgcatata acggagtagc cagccaaaat tgatgtcccg cataatgaat
    62341 tcaactgaag aaaatgcctg ctccgcgttc ggcgtataaa acatggttag ccataaacca
    62401 gtaatgattt gattgactaa aactatcagc gctaaagatc caaaaaaata aagaaaatta
    62461 aaatttttag gcgcataata attactgaaa tgtgctttcc aagtgctaac aagagggaaa
    62521 cgttcatcta accaattgac taacccattc atgccttaat ccttattttg catcctcacc
    62581 tatcacaata gtgtggtcat ctaaaaaatg ataaggtggt acttccaaat tgataggggc
    62641 aggaacgccc ttaaatactc ggcctgaaag atcaaacatt gacccatggc atgggcaaaa
    62701 aaagccacct ggccaatcgg ggcctaactc accaagatta ggtttgtatt ttggggaaca
    62761 acccaaatga gtacaaatcc cgattaaaac caaaaactcg ggattaatgg agcgaaactt
    62821 gttttgcgca tactccggtt gttgatccac caaagaatcc ggatccctca gttgagactc
    62881 atcttctttt tgtaactggt taagcatgtc cttgcttcgt ttaattatcc atactggttt
    62941 gccacgccat tcaataatag cctgttcacc aggctccatt ctactgacat caactttgac
    63001 tggagcacct gctgcttgtg ctttagagct tggtaaccag gaagtaataa aaggagttaa
    63061 ggcgcatgca gcgcctaccc cgcctaagac acaggtagta tgcaacaaaa aacgacgccg
    63121 ctcttcatcc aattgttcgt cttgattatg attacttaca tcctgattga gatcagtcat
    63181 ttcgctcact gcttaatcat cctgattatg gtctatgaac tggagccagt tcgcctaata
    63241 taactcccgt tagttataac aaagaataac aatttttcaa acggttggat aacttatttc
    63301 ttagaaaata aaaaagcctc gtataaaacg aggcttggaa aaaagtaata gaattagcgc
    63361 ttggagtact gagtagctcg gcgtgctttg tgcagcccca ctttctttcg ttccacacgt
    63421 cttgaatcgc gagttaataa cccacgcgct cttagcttgc gacggaacga atcgggactt
    63481 ggctcagcgc cttcggccaa accatcttca tcataagcaa ccaatgctct ggctattccc
    63541 aaacgaacag ctcccgcttg gccagatatg ccaccaccag ttacagtaat gtatacatca
    63601 aacttattaa gaagatctac tgtttccaaa ggttgcatta caatcatgca tgaagtttct
    63661 cgacagaaat attcctgcaa ggttctgtcg tttactttaa tatcaccttt tcctggacgc
    63721 aaaaatacac gtgcaattga actttttcta cggccggtgc cataatattg cttcatttca
    63781 gccattatac ttattcctca atctcaagtt gcttcggttg ctgcgcagta tgtggatgtt
    63841 cgctgccagc atatactttc aacttgcgat acatctctct acctaacggg tttttgggca
    63901 acatgccttt tacagcaagc tcaataattt ttgttggatt tttatcctgt aatttttcaa
    63961 aggatgccga tttgatccct ccaggaaacc ctgtatgatg atgatacatt ttgtttttaa
    64021 acttgcgacc agtcacaaca accttttctg cattagtgac tataatgtaa tcgcctgtat
    64081 caacatgggg agtatactca gctttatgct tgccacgtaa acgacgagca atctctgtag
    64141 ctaagcgacc caaaactttt tcactggcat cgacaacaaa ccagtcacgc ttgacttcat
    64201 tgccttttgc actaaatgtc ttcattaaat aaactccgaa ccgcctcaat tggtaatctt
    64261 tctatcatgg acgggcgatt ttaaccaaaa cagaaacaac ctacaagtaa aatggataaa
    64321 aaaatatata caattaatca aatctcaata aaaacatcag attaatgact aaatgtgaag
    64381 tatttttcat cttattgacg taagttcata ctgaatcaac agaaaacccc gatactcatt
    64441 ttcatctctt tgtttcagta gaagagactg ggcctaacta ctcgctcaga attaaccaac
    64501 gcttaatttt atcaaaagcc ttttcgtgcc aggaaagcac aaaaaagttt atttctctaa
    64561 ataattatct gtctagcaga agtggctcta ctatctcacc atcaattaag tggtacttga
    64621 tgatttgttc aatatcttca aaggaagaat aagtatacca aactccttct ggataaataa
    64681 cgatgcaagg accagaacta caacgtccaa ggcatcctga tttgctgacc cggactttcc
    64741 cagggccatg caaatcaaat tctaacaatt ttgatttcat gaaatcaaaa aattcttctc
    64801 cgccggaatt agcacaacat tgcttacccg gtgctttctg atttgtgcat atgaatacat
    64861 gcttggtgta atgagacaac aaactacttt cctatttttt caattttcgt ttttaatttt
    64921 aaacccggct taaaggtaac aacccttctg gcttccacag gaatttcttc accagtcttt
    64981 ggattgcggc ctggcctttg tggtttatct ctcaaagtaa aattaccaaa accggataat
    65041 ttcacatgct gaccattttc aagagcatga cgtaattctt caaaaaaatt ttcaaccatt
    65101 tctttagcaa ctggtttatt taattttaat tcgtcacaca atgtttctgc cattattgct
    65161 ttgcttagtg cgttcacgat cattccctca aaaggattga aaattcattt tccagtttct
    65221 tgattatagc actaattgtt aaattgatct cggcatcaac taaagttcga gtatcgtctt
    65281 gtagtgtcat agccacagca atactttttt tatcttctgg aataccttta cccatataca
    65341 cgtcaaatac gtcaaacgat tttaaccaat cctctttaac tgtatttctg attactcgct
    65401 caatctgcat ggcacttatc tgcctatcaa ccaggaaaga caaatctcgc cttatttgtg
    65461 gatatttgga aataggcttg tacagaggaa tagtcggatt gattaatgat tctagattca
    65521 attcaaacaa aaccacatcc tggtcaagat caaaggcatc cgacaagcga ggatgcagca
    65581 caccgatcca gcctgaatgc ttgccgttaa tcacaatctg agctgactgg ccggggtgaa
    65641 gcgcatgatg agatgattga ataaattcaa catcattcag cttcaatgaa gcaaacaagg
    65701 attgtaaatc cccttttaaa tcataaaaat caaataaacg ggcagattca ctccaattca
    65761 aattgccttg ctcccccatt aacaagccag caatacatga acgctccttt aattgccccc
    65821 cgtccaaatc aaaaaccacg cctatttcaa aaaacttcac tgcggtttgt tgtctgtggc
    65881 tattataaat catagatgca attaagcctg gccacattcc cgcacgcatt tgcgataatt
    65941 cagaggaaat tggattaagc agttccataa actctttttg tggataaagc gcttcctgca
    66001 actcaggatc gacaaaacta taactgatgg tttcatgata acctttggca ctaaaccacg
    66061 atgaaacgtg tgtcgcaatt ttttccttgg cactgattaa accagcttgt actgatgttt
    66121 gcattggctg agcttgtaac ttgtcatagc catataaacg aataatttct tcaaccaaat
    66181 cagcatcttg ttgcaaatcg actcgatgag agggaatggt gacctcaaaa aaatgattgg
    66241 tttctttggt gatgacaaca cctaaacctt cgagtaaatt tttcatttcg tttagaggga
    66301 tggacagccc cgttaacttt ttcacttttg tcgtatcaaa taaaaaggaa accgtacctg
    66361 gcaaaaactt tttgtcaaaa gactctatca ctggcccagg ttcgcctcct gagatagata
    66421 aaatcaattc cgttgctctt tcaagcgctt ttgattgcaa acaaggatcc actccccgct
    66481 cgaatcgttg tgacgaatca ctgaataagc catattttct ggcaactccg gcaattgtca
    66541 ccgggttaaa ataggcgctt tccaggaaaa catgttgtgt cagctcttga actgcgctat
    66601 tagctcctcc cattattccg gccatagcca aaggcttttc tttatcagcg attaccagca
    66661 cattgtcatt taatacaact tcttgaccat ccaataattc taattgctcg cctgaagcgc
    66721 tataacgtac attaatttca ccgttaattt ttgctaaatc aaaagcatgc atgggttgtc
    66781 caagctcgag catgacataa ttcataacat ccactacagg gtgcaatgtt ctaatgccgc
    66841 ctctacgtaa gcgctcagcc atccataatg gcgttttcgc ttcaagattt aaattacgga
    66901 taattcgacc gcaatatcgg gggcatgcct cggaatgaat taaattcact cgaagaccat
    66961 catcgatggc tggcgccacc gtaactattg gttgctctat taagggtaat ttattgagaa
    67021 cagccacttc ccgcgcgaca cctaaaatac taaagcaatc tgcgcgattc ggagtcaaat
    67081 caatatcaaa tacatggtca tcaagggcaa gatactcgcg taaatccatt ccaattggtg
    67141 cctcatcact cagttccatt atcccctcgg aatgttctgc cagtcccagt tctgtcgcgg
    67201 aacacaacat gccctgagac aattctccac gcaatttgga ttccttaatt tgcaaaccgc
    67261 caggcaagtg tgccccaatc atggccaggg caactctcaa accaggcctc acattagccg
    67321 caccacaaac aattttcaac ggcttgtctt tattgatatt cacttcacat aaggttaatt
    67381 tatcagcatc tggatgaggc ttggtattta ggacttcagc aacaatgaca tgagtgaatt
    67441 ggccagctac aggattcact gcgtcgactt ccaagccagc catggtcaat tgctcagcca
    67501 gttcttgttc agttaatgag aagtttaccc attctcgtaa ccataactta cttactttca
    67561 tttaaagtcc tcatctctgt aactgttcat ttggctcaaa gtttccatcc gttaattttg
    67621 ctctctttat gataatagag ataaaattac tgataactat ttgcctgtat caactacata
    67681 ataaaatatt attttcagta ttcatgggaa ttatcccggg ctacaatttg gtctaatctg
    67741 tatcatttat gtaccatatg aaagacaatc ataaagaaac ataaaaataa aatccgggac
    67801 tcattatttt tataatggtt aaaactgccg taaaaaagtt aaatcatttt caaataacat
    67861 acgcaaatca tctatgccat aataaagcat tgccaatctg tccataccca tgccaaaagc
    67921 ccatccatga tactcattcg gatcaatgtt cactgcgatt aaaacgttag ggtggaccat
    67981 accacaaccc aatacctcaa gccagcctgt aaacttgcaa gagcggcaac ccttaccatt
    68041 acattgagta cattctatat ccacttctgc ggaaggttcg gtaaaaggga aataggaagg
    68101 tctgaatctt aatgccaatt cccgaccaaa aaaataagcg aagaaatctt gcaacaaacc
    68161 ctttaacccg gccaaagtgg cttgcttatc gatgagtaat ccctccactt ggtgaaacat
    68221 aggggtatgg gttacatccg aatcacaacg ataaactcgg ccaggagcaa ttaaacgaaa
    68281 aggtggctta cgctgctcca ttgtcctgat ttgtacagga gaggtgtgtg tcctcaacaa
    68341 gcgcccgtct ccaaaataaa acgtatcgtg catcgcacgt gcaggatgat ggccagggat
    68401 atttaatgct tcaaaattat aaaattctgt ttcaatttca ggaccttcaa caatatcaaa
    68461 tcctaaacga ctaaaataat cattaattct atgctttacc tgagtaacag gatgtaggga
    68521 acctgtggaa tgattgcgac ctggaagagt aacgtcgatt tgctctgctg ccagcttggc
    68581 taataattgc ttttctttca actcgatcat ttttgtttca atcaaagcac taattccctg
    68641 ctttgcttga ttgaccaatt gacctacctt gggtttttcc tctgcagaca aattagcaag
    68701 tccttttagc aattcggtga gcttgccttt cttgcccaaa aaatcaacac gaatttgttc
    68761 aagagcaact atatccgtgg cttgtttgat tgcttctgaa gcttgttctt gtatggtgtt
    68821 tataagtacc aacatagttt tacccacaag taaaaaggag gccttagcct cctcaataat
    68881 ttttatttta caaaccggct tttatttaag attgtcattc cgaattgaga tttataaacg
    68941 aaaggcaata acaaataaag cgggctgaaa attaaccagc taaagctgct ttggcttgtt
    69001 ctgcaatagc agcaaaggca actttatcat gaaccgccat atcagccagg attttcctgt
    69061 ccagctcaat ggaagctttt ttcaaaccat caatcaagcg gctgtaggat aacccacatt
    69121 cacgagcttg agcattgata cgagtaatcc ataaagcacg gaattgtcgc tttttctgcc
    69181 ttctgtctcg gtatgcgtat tgaccagctt taattacagc ctgtttagca actcgatagg
    69241 tacggctacg ggcaccataa taacctttgg cctggtctaa aattttcttg tgacgcgctt
    69301 ttgctgtcac accacgtttt actcttggca ttgttcaatc tccccgttaa ctaccgtgca
    69361 acatacgttc agccaaacgc gcatcgcatg gcttcaaagt acctgcttct acacgcaaat
    69421 gacgcttttg cttagtagat tttttagtga ggatatgatt tctgtaagcg ccgcgacgtt
    69481 taatcgctcc actcgctgtc tttcggaagc gtttagctgc tccacgatgt gactttaact
    69541 tcggcataac aatatactcc gcattcgtta cttggttttt ttgggtgata ataccatcag
    69601 taattgtctt ccttcacgtt ttgcatgctg ctccactaca gctacatccg ccgtgtcggc
    69661 ttgcaaacgt tccaaaatct tcataccgag ttcttgatga gccatttcac gaccgcgaaa
    69721 acgcaatgtg attttgacct tatcaccatg attgaggaaa cggatcaggt tgcgtagctt
    69781 gacctgataa tctccatctt ccgtcgtagg acggaatttt aattctttga cctgaatttg
    69841 tttttgcttc tttcttgctt cagcttgttt tttactcagt tcaaagagaa acttaccata
    69901 atccataata cgacaaacag ggggtttggc tgttggagat atttccacca aatccaatcc
    69961 gctttcttct gctgccctta acgcttctcg cgttgataca attcctgcct ggttaccatc
    70021 gacatcaatt aagcgtacct caggtacagt gatctgttca ttaattcgtg cgcgatcatt
    70081 ttcacgctta gttggtgcac taatggttta atcctccagt tcgtttattt atattgatcc
    70141 tttacggata atttcttgcg ttaaggtatc acaaattgta tctattgtca tcacacccag
    70201 gtcaatgcca tctcgagtgc gaacagctac ctgacaattt tcaacctctt tatcgccaac
    70261 aaccaataaa taaggaattt tttgtaaagt atgctcgcga attttaaatc caatcttttc
    70321 atttctcaag tcaaaattgg cgcgaatccc tcttttttgc aaagttttcc tcactttttc
    70381 ggcatactca ttctgcttct cactaatagt aagcacaacc gcttggactg gggataacca
    70441 taatggcaat ttacctgcat aatgctcgat taatattccc ataaatcgtt cgaaagaacc
    70501 caaaatggct ctatgcaaca tcaccggtgt ttgcttgctt ccatcttcag caacatagct
    70561 tgcctcaagg cgtgcaggca tagagaaatc gacttgaatc gttccacact gccaaattct
    70621 acctaaacaa tcagagagag agcattcaat tttgggtcca taaaatgctc cttctccagg
    70681 cgcatccacc cactcaatat ttcttcccag catagccagt ttaagcgcgg tttccgcctt
    70741 atcccaaacg tcatctgaac cgactcgttt ttcagggcgc aaagccaatc ggtattttat
    70801 ttcagtaaac ccaaagtcct tatatacgga ttgcactaac tccagcatca tagccacttc
    70861 agattgaatc tgatcctcag tgcaaaaaat atgagcatca tcctgcacca tattgcgtac
    70921 ccgcatcaac ccatgcagcg cacctgatgg ctcacagcga tgacaattcc caaattcaga
    70981 tagtctgaga ggaagatcac ggtaactttt caaaccatga ttataaattt gaacgtgaca
    71041 gggacagctc ataggcttta ctgcataatg tcgattctct gtttcggtaa caaacatttc
    71101 atctctaaag ttcgcccaat gacctgattt ctcccataac gatctgtcta ctaattgcgg
    71161 tgttctaatt tcctgatagc caaaatcgac caggcgattt cgcatgtaat gctctaactc
    71221 ttgataaata gtccatcctt tggggtgcca aaaaaccatt cccggagcaa tatcctggaa
    71281 atgaaacaaa tccagtgcct tgcctaattt acgatgatca cgtttttctg cttcttccaa
    71341 acgaaacaaa tattcttcca gagatttttt atcagcccag gcagtgccgt aaattcgctg
    71401 caacatttcg ttatttgagt caccacgcca ataagcacca gctacttttg tcaatttaaa
    71461 tgcttttaaa aacccagtgg aagggacatg tggacctcga cacaaatctt caaaatcacc
    71521 ttgcctatat agagagagcg cttcattttc gggtatatca gcaataattt ttgccttata
    71581 ttcttcgcct aaacccttaa aatattgtat tgcttcattt ctaggtaatt ctcgacgagt
    71641 gattgaaaga ttagcctttg ccagctcatg catcttcgct tcgattaaac tcaaatcatc
    71701 gggagtaaac gagcgctcaa aagcaaaatc ataataaaac ccgtcttcta ttacaggacc
    71761 tatagtcact tgagctgatg ggaataaggc ttttacagcc tgggctaata aatgagcggt
    71821 agaatgcctg attatttcaa gactgtcttc atgtttctcg gttacgataa tcaaagagca
    71881 gtcttctttt ataagatagg atgtatccac caagacgcca tcgacacggc ctgctatcgc
    71941 tgctttagct aatccagggc taatgtgatg tgccacatca taaatagtga gtggcgcttc
    72001 aaaatgtttt acatttccat ctggtaattt aacatttggc ataatcttat ccgtctttcc
    72061 atctttcagg ttcttgttaa aaaaaaaccc tgcatggcag ggttcaaacc tttaataatt
    72121 cagtgcagta gcttaccaat tagagattct actagaaatc caggaattat ggtaggcacg
    72181 agtgggatcg aaccaccgac caccaccatg tcaaggtggt gctctaccac tgagctacgt
    72241 gcctataatt caacctgaat gaaaattggc atgaattata cacataagat tcatggaaat
    72301 gcaatcatcc agatcatatc atttttatct aatgaaaaag caagataaca tgattttaaa
    72361 attttggatg agcttggcct atcaatcttg ccttttaact cttttgcaaa tgcaaccctt
    72421 ctcaaccaaa ccgtaaaata ctcttgcttt cccctaactg cattagtgca agaagcataa
    72481 agagcatgaa aagctaattc caactctctt gggaacaagg taaaatgata aagcctccgt
    72541 tagcatatta aaatattatg tggtttaaaa tcatacattc ccatgtagaa atagctattt
    72601 atgccaaacc gaacgccaag aagacaaaac tcatggccat ttctgatgat aggctgcata
    72661 ttgcgctcca tgccaaaccg caggaaggag aagcgaataa tgagctttta ttctttatat
    72721 cccaattttt caaaattcca aaaacacaaa tcgaactaat taaaggaaaa agcagccgtc
    72781 ataagctaat aagattaccc ttatcagaat ctgtgttctg ctttctaaat aatccaaaaa
    72841 tctagaacat atcacccttt aaaacttaga aatggcccaa atgaaggggt tcattcaagc
    72901 caagcaatgc gaggttaacc gctatggtta aaccactttt tctgttgatt ggactgccct
    72961 gcgatacttg ttccttcaag taacatcagc gtccgatatt tggacttaaa gaaacgtgtt
    73021 ataacgttcc aacgcccatt ataatcattt aacaccaaaa atcaactaat gatttccaat
    73081 agaaacaatt tgtttccagt ggatggatat atttgcaggt ttaactaccc tttgctacac
    73141 ttttgtcact ttttaatatc gtaaaattat tactccaata tggaaaaagt taatttgaca
    73201 aaacagtttg cttaccgctt gcgtgatgcc atgattgcag cagggcataa ttctcaccgc
    73261 tctccatcag gagtatgtat ccataaatta gctgaaatga caggttactc tttgcaaata
    73321 tgcagaaaat accttcgggg agaggttatt cccgatccag tcaagctgat agaaattgca
    73381 tcgaaactaa atgtttcgcc aggatggctt ttgtttggtg aaacccctaa tgatcctgga
    73441 acatctaagg aacacatcac gataagcaaa agtttactcc atcatatttt tacaaaagca
    73501 gcttgcctat ataatcgtga gcttgtggaa gatgatgtgt ctgacttttt gatggatttg
    73561 atcaatgaca ttagtctcat caatgccacc gaagaacagt ccaaaaaaat aattgatttg
    73621 gctatctctt cagtgagcca ttttagacac tcgcaaggaa cgtagagtaa gaaactttca
    73681 tggaaaatag ctcaaaagag atagtgctcc actctcaact tttagagaac cttttcgcct
    73741 ttaaaagtaa agtatccaat gtctttagag acgtattggg catacatgac gttcatcata
    73801 tttccttaac aaatattaac gggcacaatc aattgctgcc cctatcctct actcctgcca
    73861 tggattttaa cttatttaac agttttcttt ggcaacatga cctaacctat aatcccaagt
    73921 gatttaaatt atgtgctcaa ggcgattggc acacacttta ataatataaa acactatgat
    73981 gagctatatt atcttgatca aattaaacat ggttttccca tcggtttctc ccttgctacc
    74041 aagataaatg aaaattatgt gatttattca atagcaagcc accaatcttg tgcctttact
    74101 cgtgagttat tttctactca tcaagaagat tttccttctc aatatttaat tttaacctca
    74161 cactgaataa taaacataca tcccgacttt gtaatgaatt acttgatttt taaagagctc
    74221 gataatctac gtacatacca caattttttc gaagcatttt gtgtgcactt tatggctatc
    74281 tctaaaactc agtgcttcat agagagtcgg tcatttgccc agaaacgcct gattaggaat
    74341 agaagtattc tggtctacac tcagcgaacc tgatactgct tttggtttcc cgagcaagct
    74401 cagttttatt aaactctcct taattttaca atcgagaata tctgttgcca attcaggtga
    74461 cgtcttaggg attttgtgtt ttaaagtgta ttcatccgaa tccagcactt tataccttga
    74521 tatattgcga agaaggcttt cacttgctgt tttatccaca ttggtgtgtc gattttcagt
    74581 caaaaactgg aaggctttgt taatttgctc ttcctcaccc attcttgagt attttccaca
    74641 cagagtatcc agattataca cataggttaa tgggctttga aaaggatcat gcccatgaac
    74701 ataggtgaca aaataaccat tgtattttcc tggtcgagca ttctctactt ctttgctttc
    74761 attccagcga ttccatacta aataaattaa cggcgatgcg tttctctctt catcagtcat
    74821 attggttgga tcgttaattt cattattctc aaataacaaa tgcagcatgt tgttcttcat
    74881 ataaatttgc aattgggcat taagttggtc tatggtttca gccaatgcct ctttggtgga
    74941 atcattataa gtgacaccca actgagaagc tgccattcga atgctatcaa agcgtatggg
    75001 agcatggcta tacaaggtaa taccgtcctc gcttagagaa taatcgataa tttttaaagt
    75061 tggtttataa gctctctcga ctaaacttct caattcctcc tgggtaatgg cactttgctc
    75121 cagtaataat tggatacccc agaatgattt aatttgtata tccccaatat aacctttggg
    75181 tttaaatggt tgaccgatta caagtttttc ataagcatga ataaactcgt acccatgatt
    75241 ggataaaatg atcttgattg ctatctgatt ttgataaagg aagtccagta ttcttaaggt
    75301 gaaataatca caactccccc gatcggccac ctcatcaccc agcaatcgaa caagcgtttt
    75361 aatatcatta atttctatct gcgtcatgaa cttattaaat cgctcaatac atgatggcag
    75421 ccttgttttc aacccactta atttttcttc caggccagct ttactctttt cagccatttg
    75481 taaattggcc tctattggtt tcttaagttg caataatgat tgagaaaact ctttctgatg
    75541 gtctatggca agtgataatt tttgctcaat gtctaaaatt ctttgctgtg cgttagttat
    75601 ttttatttgt atcaattgaa gaagcgtacg aatttccaaa tactcttgaa ctatatcgtc
    75661 atattgttca taaattgtta caaattgctg gtaagcttcg tggaaattgc t
//
LOCUS       DAXLDT010000002        57414 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXLDT010000002 DAXLDT010000000
VERSION     DAXLDT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 57414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57414
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.002"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            89..907
                     /locus_tag="ACF2SP_000076"
     CDS             89..907
                     /locus_tag="ACF2SP_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357892.1"
                     /translation="MFIKSPCIDLTRHSKIWINPDGEIPKKIVERLKWQKETRPRDAI
                     TLFVNKACEDKSNSAVESLRACGVKIKIIELCLEKNEKQDDPFIIACFNKALALAKKE
                     KNLVDRVRASVRATNVLRLMKLVQHEGLYSDNDVLFLKFEATRLLSPYLFGQYEGEVN
                     DVHLFGVAINDPLTTDYFYTRLVEKMKKPWEEEITPDEFEPPCGLYLIPDEIISKIQF
                     GHLKFAEIRDCIITGSDQSHHDITRAKKLLNLEEDSLLDEAKSIVASQEKQYRM"
     gene            complement(1082..1924)
                     /gene="htpX"
                     /locus_tag="ACF2SP_000077"
     CDS             complement(1082..1924)
                     /gene="htpX"
                     /locus_tag="ACF2SP_000077"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HFD2357893.1"
                     /translation="MINNLKTFILLASLTALLVVIGGLLGGSTGMLVALLFAGIMNFS
                     AYWYSDTLVLKMYNAEPLSNNHFVYHIVSELAHRAGTSVPKVYLINNSTPNAFATGRN
                     PENASIAVTTGLLDRLTQEEITGVLAHELAHVIHRDTLINVVSATIAGTISGIANMFM
                     WLSMFGHSSNNEEGVHPVVGMIMMIVAPLAAGLIQMAISRSREFEADAGGARISGNPQ
                     WLASALLKLDQANHEQYFDEAETHPATAHLFIINPLNGEKLANLFSTHPSTAERVARL
                     RAMY"
     gene            2133..3044
                     /locus_tag="ACF2SP_000078"
     CDS             2133..3044
                     /locus_tag="ACF2SP_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357894.1"
                     /translation="MYSKYPVFFPNKNIESFSGVRFSNVVGIPSAIESLYRGDNNLAG
                     IIFLLPTLITGVFCQSFPEVVDMEQVRLHKLTNLSNDFHMVSMSEDPQIALDWGKGCF
                     ITIDPVLFSDYIVDVHATFRKNQLNFPDRMEQEREHIALAVPFCSIKKITIDNKELAN
                     PFYLSIPQDNHEARMEFNTLYSELISLLRKKYTQEVDGEEEKVALRAYAIRYLDFYSK
                     FGSCNNPFNKSLAQLVELYPEFMSNFLQSSHLSSKTGLMKDIVLNSSDNLFKEHPYTQ
                     SIDASYIHRVKESTTCYEDDWAKPVYD"
     gene            complement(3091..3522)
                     /locus_tag="ACF2SP_000079"
     CDS             complement(3091..3522)
                     /locus_tag="ACF2SP_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357895.1"
                     /translation="MKKSSFFDSSFFTTSDTKEEFPLFPEEGMDCLFEKLGSRITSKK
                     TGNTYFITPADPSIVIQKGNDGVHEYYHLILNKKVIAVSSFVNHKTTIYLANIIKQLE
                     PERIGTPVDFDFLGDTDETDSVFSDTLDNTEGNIDQMPSPR"
     gene            3954..5432
                     /locus_tag="ACF2SP_000080"
     CDS             3954..5432
                     /locus_tag="ACF2SP_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2357896.1"
                     /translation="MNIAIIGGGTGLTTAWALENQRKFHVTVFEENDRLGGHIHSIKI
                     NNVILEGGAEFIGPPALYPNVHRLFQSLGMALDQFELNMDFDNLKQRDHIVLPPLYHT
                     SSGKSEDVSSILYTLSCGLFGKRRKQNETRVSVDTMFTEMYNLLEMNHLIMDARNKLL
                     HPDNLMTLEQFINEFKEKCVHVLSPVDKFAEEFVYPLIASGWGVSIETIKKFGAHYAM
                     NYLAAGMNWFDAPEGLSSYIDKMVAQCTNTQFKVNTAIKKLIPITIDGKIKYQLLKRD
                     DTFVCDSTGSPMIYDDVVISTPAYVTNELLSDIESNTIKVLREKLANVTYYDTTVVFH
                     QDPEYLSPYRTVVHSRFDGTQSANTMCKPWKFNKDEIPIMKTWVLPGQEMPKNVLREV
                     HYKHPVMDKNYYEAQQALHAAQGEAGLWHGGILAGFNDSHESGVTAALQIAAHLNQRE
                     HCLDKNERLALFPHILDSVLSPKLSTDMGVEVAGVVAYPSMP"
     gene            5692..7101
                     /locus_tag="ACF2SP_000081"
     CDS             5692..7101
                     /locus_tag="ACF2SP_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFD2357897.1"
                     /translation="MIRKKITQISFFMILALIVGAVEAKTTRPADEQQLARSIYKQFI
                     EVQSGFTTGSTTPLVEVAVKYLKKAGFSNEDIFVGGASPQKANLVVRYRGTGEKKPLL
                     LLAHTDVVEAKASDWSMNPFQLTEKDGYFYGRGTLDDKAQAAIWIANLIQYKQEGFKP
                     KRDIIVALTADEEGSSPYNGISWLIKNHKDLIEADFALNEGGWGDLANGKKISQNIQV
                     SEKYIVNYNLEVRNKGGHSSLPTKDNAIYRLAGALERLSKFNFPLKTNDVTAAYFKQM
                     AAIETGPLKTEMVEASKGSKQAMQRLADSSPQWNAILRTTCTPTLLEGGHAMNALPQL
                     AAVTINCRVLPEDSPEMVEQSLKTAINDPEITLKRIGKLSRGPSSPLSPEILKTITHL
                     TQTYWPEVPAIPIMVTGATDGRYLRSVGIPTYGVMGLFLDRDDFRAHGRDERISVESF
                     YEAQAFLYDLVKQLSSNVT"
     gene            7228..8280
                     /locus_tag="ACF2SP_000082"
     CDS             7228..8280
                     /locus_tag="ACF2SP_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HFD2357898.1"
                     /translation="MLKGLKVIDLSTVLAGPSVGTFFAELGASVVKIEHPVHKDVTRT
                     WKLPGEDPHSEISAYFASVNFGKDYLFLDLMKEEDYLFFLNLIAEADILIMNFKKGDD
                     IKLRIQADHLLSINPRLIIGKINGYGEESERVAYDLVLQGESGFMSINGTPDSGPVKM
                     PVALIDVLAAHHLKEAILLALYNREKSGKGKVVSVSLYDAAVSSLVNQASNYLMTGQV
                     PQRIGSLHPNIAPYGELFRTADAALIILAIGSDRHFAKLCTVLGLNDLPDNDEFKHNQ
                     NRVRNREKLALLIAGQTAKWQSNKLLEMLHRENVPAGKIMDLKDVFDNERAKSLIRKE
                     LIGDVPTARVTSVVFK"
     gene            complement(8477..9343)
                     /locus_tag="ACF2SP_000083"
     CDS             complement(8477..9343)
                     /locus_tag="ACF2SP_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HFD2357899.1"
                     /translation="MKPGYLLGALLLSPLLAFANSDNNADGLIFSPLPQNKNTVVRHY
                     SNEQDMSNLNQMTQRTIDFPTQIVRVSGNLTGLELSCDDVENEIDQIFSKKISPNLFT
                     YNTYVSCGFDEHDPEQYATNFSIQSYFDPLTDNAVDYLKNYLKEYNGYNLFNTTTLQI
                     ENAKGIIVSMNLNAGLKNNPDKTPFMLYRQDRNNFYFKSNFDMRKELITDIYQRFYSN
                     DPDMILPFFDKWIFSYAGSVYYSILKASNYLELQPERIFVMENEGDIFVSDLRYYFAN
                     LCMKRNSNKHCL"
     gene            9722..10675
                     /gene="ceg5"
                     /locus_tag="ACF2SP_000084"
     CDS             9722..10675
                     /gene="ceg5"
                     /locus_tag="ACF2SP_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg5"
                     /protein_id="HFD2357900.1"
                     /translation="MISQKEALVLLNKYEKHLNELQNIITTSYQNLLRCDAQIIPNAL
                     AALKAIANNTQFVPVVATSGMSSIKALSSNPQIALKELLVNDFFKDHFQKMCIHYAKD
                     KPELLTNLQNFHACVSQEKIIIYKAKNGELALKFPTQEWRDRFIQKMGGVSHFASSDK
                     FNANKECPQTYPENLTTLYLSTYTAKNGELAVVFPNKEIRQNFLTLLRHASSATWSIY
                     EGQDAVYFNDKNLHTPGYNFGINAPACVSQSSNAAHADVRYAARVLGQALRNPNNTCF
                     FNRLPEELINKIITLSTDNEVLAAEDAISSVQPNLGKPQLK"
     gene            complement(10910..13159)
                     /gene="katG"
                     /locus_tag="ACF2SP_000085"
     CDS             complement(10910..13159)
                     /gene="katG"
                     /locus_tag="ACF2SP_000085"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFD2357901.1"
                     /translation="MFKRTIPLFAAFTLAISPSVFPNYAYAQEDKPKTNQYWWPKMLD
                     LSPLRQPNATSNPMGEQFNYAEEFNSLDLNAVIEDLKKLMTTSQDWWPADYGNYGPLF
                     IRMSWHAAGTYRIYDGRGGANGGFQRFAPQNSWPDNANLDKARRLLWPIKQKYGRKIS
                     WADLLVLAGNVAMESMGFKTIGFAGGREDAWEAININWGPEGKWLESKRQDKDGKLEK
                     PLAATVMGLIYVNPEGPNGVPDPLAAAEKIRETFGRMAMNDEETVALIAGGHAFGKTH
                     GAASGKYLGPAPEAAGIEEQGFGWKNSYGSGKGKDTITSGLEGAWTVTPTHWSHNYLQ
                     NLFNFNWVKTKSPGGAIQWVPENSNASSMVPDAFDPSKRHAPVMLTTDLALKFDPVYS
                     KIAKRFLDNPKEFDDAFARAWFKLIHRDMGPRSRYLGSLVPKEIMIWQDPVPPVDYKL
                     VDANDIANLKGKILNSGLSTSELVKTAWASASTFRGTDMRGGANGARIRLSPQKDWPA
                     NDPQELAKVLKTLESIQNNFNNAQADGKKISLADLIVLGGNAAIEQAAKQAGYDIIVP
                     FMPGRTDATQGMTDVKSFEVLEPKADGFRNYFDKSNNMSPPEMLVEKASLLKLSVPQM
                     TVLVGGMRVLNANTGQNQYGVFTDKPGTLNNDFFVNLLSMSTEWKKSSETEGIYEGYD
                     RKTGKLKWKATSVDLIFGANSELRAVAEAYATDDAKEKFIQDFVNAWVKVMTADRFDI
                     KAANTNINS"
     gene            complement(13279..14283)
                     /gene="ravE"
                     /locus_tag="ACF2SP_000086"
     CDS             complement(13279..14283)
                     /gene="ravE"
                     /locus_tag="ACF2SP_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavE"
                     /protein_id="HFD2357902.1"
                     /translation="MEHCMPKTKYALPPVVLYESHADRATSDFLIKQLPDLKKTGYTT
                     ICVDGMEPGASLEENISMMKILIKIQIKKLSELPLEHPEYEQGIEKLRSVVAKLELFE
                     AMKEQGFKLGGIDLPVSEQLKEKSLNSIRREQTLTDNTLGHVKENDGGVVVVLGFGHC
                     IFQQMIKEQDENADQYLWYHVHNPDNETQAYKELVKSYTKKGLSTYFPLGVNIFKSSD
                     KELDTDFWNKVSANCYNYDPKALETSTASILKSLLGPEVSAHLRTDGHHHVDALISLE
                     TVEKTHQVKSSDFLRSLSKTLGDIHFEVAKIKTKDQVIIRGINEPEVAEQISKLSKKM
                     "
     gene            complement(14570..15823)
                     /locus_tag="ACF2SP_000087"
     CDS             complement(14570..15823)
                     /locus_tag="ACF2SP_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357903.1"
                     /translation="MSLEIYTWKNGSKGLGFTKPVKSMLFGGNVGHAAVELTFPADAK
                     GDELAKKYQDVPGLSISKRTEIVPEKQEDGSYKPKEQVVYFVYFSWWPGHTNGHHINS
                     HREDLESEWRHEPAPKIKPEIQKHLYGENTPAANKTNVTGILISEKTISKVKEFSHQN
                     IKETITIENDPAYQQLKAKEAALQTELQQLIYKGKIYEQELAKSALEQRQPNKDLGLT
                     DAEIVRLAEINKELKRVVEQIKLCQMDFEERYLSVGEPPSSVIRIPTTHDYNAPTFAL
                     DTAGILEKMASLAQSTTAYSFYKFNCSTSATQVVKAGISDELKNIMKTDGFNVDKASQ
                     TTIATPTSFSHFSQQVQTELILLNTNQALVEQQRAKTANTSGPLTTTTQSFKQKFQEA
                     VKGKSEEIVREQDESEENRMDLTMS"
     gene            16215..16532
                     /locus_tag="ACF2SP_000088"
     CDS             16215..16532
                     /locus_tag="ACF2SP_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HFD2357904.1"
                     /translation="MTMMITTGNSFDGKVIKQCLGIVRGIVVRSPTISQGLMGGLKSI
                     VGGKIGAYSQMCEHAREEAFQLMIEHAQALNANGIIAMRYDTGEIGQAGTEVLCYGTA
                     VII"
     gene            16781..19432
                     /locus_tag="ACF2SP_000089"
     CDS             16781..19432
                     /locus_tag="ACF2SP_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFD2357905.1"
                     /translation="MMPIDINKYYSLIVKMPCPSEGGPTLNLAFGKDTAKRWGVVFLQ
                     RIKTPNGLGTVIGEYKDRLWIWLDDNRGVSRWEVKDNLFSMKGFEPVKKPSMKSYPDE
                     VEKYIENLFQCSINELVTFTQNPFLLIFGEESKQAIEAIEKNWGLWLEYTTTNLNTWS
                     NYFTKYLQEHSQNPLEKMQQYGELTSRVTGIPKITIKEFNLSYVKEIKEVEEQIFELQ
                     QQKSSLLEQTTAKEDQEQIKGMFKNLEQVLHDKLEQLNKLEEQNRRQKMLTSQLNKVL
                     TNQQFAEFNQHAKRIELTVNSLLAIKPQLQDKHFMKTYQAMLTLMAQYLYEQDLIEKQ
                     SEATDKTGKEEAEANLSPDLFTGLVFAMVAGDSTIKKAYYLRLINRLDNNNNAAKEMP
                     VTFDLEIWAVSNPQQMINLCRFLFNNSKFKQFEEGLSYKEYPLFIFEIVKLKMSFILA
                     LNEVQKNNSKFSIPQEIKFHAQKTIDLLVDLILDGNEEIAIWAEKQIKGNKNPFIKNY
                     RVYAALAEFYATPPHVNEEKGLFFYNEGKTSTKDPEENKQAKLVSILYEAIHGSTGEI
                     RRAAVELLNQKREYHYLKLVGHKNPDAALLCLDYCLANNQLDEAKQYITLARKADRAK
                     AAYFEALLVDKQKGDFMPFFLEAIEFGFAHAGYALRKMLSVLLEKKQIEEINERYLNL
                     LNKPFVLNILTVPVIKDTIMAVYNSTGDTQQQEEFREKWMFNLLVVVKNTEIVQKTDL
                     VVTIITECRELLSDEGLLVALSILSKQRYFDEPGDPFLLDAIAQIITEQDKKGPKHFF
                     NLGIYDFLKNNTKLVVLLMNNSSSKERLAQCERFTSFIKKEQIRIEESDKVKNQPSLA
                     NLGLFGDNNNLPEEKGPSPSPMGTKKG"
     gene            19847..20983
                     /locus_tag="ACF2SP_000090"
     CDS             19847..20983
                     /locus_tag="ACF2SP_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFD2357906.1"
                     /translation="MKKILGSIGLAVVSISLFAHGSMEVPISRTYQCYKEGPESPKSA
                     ACKAAVQVGGTQPLYNWHEVNQAAANDRHRELIADGQLCAGGRDFFKGFNLARTDWTT
                     TVVHPDSNGRFQFVYVATAPHKTKYFKFYVTKDGYDFNTPLKWSDLETSPFCTITSVT
                     LANGRYQMDCPLPANRTGKRIFYVIWQREDSPEAFYSCSDVFIDSTAQAPTWFELQNF
                     YGSGDIAANTKITLRVFKHEVEVESTSITVTESNRTAEQWPLALGAAVNNRSSLVRIG
                     QLDPQSGQVVLAANQANKIYLSDSDTSSYHVLVDFAEPSGNSDLIYPDGISTYKAGTI
                     VLGRLDRKYYQCKPWPYSGWCSQAPAYYEPGVGLAWREAWILLG"
     gene            21361..22395
                     /locus_tag="ACF2SP_000091"
     CDS             21361..22395
                     /locus_tag="ACF2SP_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357907.1"
                     /translation="MTIMRVFLSFLLLFFNSLSFAQSELPQLFLFLGGDRASTFQETL
                     QNPCIRGVQIIYSWRQLEPQKGVYDFSKIEEDLNDLSRIDKKLFIQIQDRSFSPDVFP
                     VPDYIRMEKAYHGGVVQQYDFPGEGKPITAGWVARTWDPAVRKRFQLLLKKLASKFDG
                     KIYGINLPETAIDLDPNHLPEDFTSDKYFYSVLDNMGYLKKVFQKTKVIQYVNFFPDE
                     WNNDHHYMSRLFSFARSHHIGLGGPDVVPYKESQMKNSYPFFHKFKGEIEAAIAIQEP
                     DYTYTNPKTGDFYRFDDFYSFSKEYLGAFILFWNTEEPFFSEQLLPKLNDSYFQCDKV
                     NDKANTADAN"
     gene            22508..23878
                     /locus_tag="ACF2SP_000092"
     CDS             22508..23878
                     /locus_tag="ACF2SP_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFD2357908.1"
                     /translation="MLVELLSRIQFGFSIGFHILFPTLNLGLAVFLVIMEALWLKTKN
                     PVYLEICKFWTKIFALTFGMGVVSGIVLAYQIGTNFGPFITQFGNVLGALFAYETLTA
                     FFLEAGFLGVMLFGWKRVPPALHFIATLLVTIGTTVSAFWIMSANSWMQAPSGYQLIE
                     GKYVVDSWWAVVFNPTFIPRFIHMLLASYVTTCFVIMAVSGFYFLRKQSTAIAKKSLS
                     FSLWASLILVPLQIGVGDVVGINVHHYQPLKTAAMEGIWETQKGAPLLLFAIPSQTQQ
                     KNFYEVGIPKLASLINTHEWNGEMIGLSSVGNEEQPRMAPVFFSFRIMVGIGLIMLFT
                     AIAGFILRLRGTLFENSWFHRWCIVMAPLGFIASIAGWLTAELGRQPWVVHGLLKTRD
                     SVSAIGVEEVIISFVLLIVAYGVVFGFYLYYLLRLISHGPGILEEDEVEHHAFQYMTD
                     FPKEKK"
     gene            23878..24867
                     /gene="cydB"
                     /locus_tag="ACF2SP_000093"
     CDS             23878..24867
                     /gene="cydB"
                     /locus_tag="ACF2SP_000093"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFD2357909.1"
                     /translation="MLPFIFATLLGFIVLMYVILDGFDLGIGILFPFTSSESERDKMM
                     NSIAPVWDGNETWLVFGGAMLYGAFPQVYGLLLPILYMPIMLMLIALIFRGVSFEFRF
                     KAKRSKTLWNWSFSISSVAATFLQGVILGAFVQGFSINESSMTINDPDWLTPFSLCTG
                     IALVCGYALLGATWMIIKSTGRLQRKMVHYARGLLVLVSFFLLFVSIWTPLHSEEVFH
                     RWYSFPNMLLLSPLPLIALIAIYLVWQNLSSGSEYKPFIYSIIIFLCSYIGIAISVYP
                     YLIPHQVTFWEAAAPDSTLVFILIGVAIMLPILLAYTLYAYYLFRGKSSENYH"
     gene            complement(24868..25653)
                     /locus_tag="ACF2SP_000094"
     CDS             complement(24868..25653)
                     /locus_tag="ACF2SP_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HFD2357910.1"
                     /translation="MNKMIILLVIAIIFLHMCLIWLWYRYTNNPSVVDVGWASGLTLA
                     GLIYLNNQEFSLRSLLLVLALLTWGIRLGGYLWWTRIRLKKIDKRYLALSNDWKLAKP
                     LGFFLNFQLQGVLICLVSIPWYFSSSETQTTLNLLDGLALIIFLTALILETVADNQLQ
                     DFKKNYPGKVCNQKLWRYCRHPNYFYEWLVWCSFTLFALSSPYGWIAIISPLTLYLIM
                     TKITAPMTEQGSIESRGNLYIEYQKVTPMFFPKWLKNNSTPRK"
     gene            complement(25650..26186)
                     /locus_tag="ACF2SP_000095"
     CDS             complement(25650..26186)
                     /locus_tag="ACF2SP_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HFD2357911.1"
                     /translation="MIINPVHIVHYLSYYLCWIFCIYLASYGYPLLGLIFTVLIMGLQ
                     IIVQILNQLPWRDALYFSIGLALLGAITDAIWLYSGMIYFKANFFGSHFPPLWMVALW
                     LSFGFNLVVLYGSWLTYYFLWGLVMFPSIIFAYWLGVQFGAATLIGSEVVFYLTLGLT
                     WSLFLPLSLYLFNYLHRK"
     gene            complement(26176..27963)
                     /locus_tag="ACF2SP_000096"
     CDS             complement(26176..27963)
                     /locus_tag="ACF2SP_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFD2357912.1"
                     /translation="MPLDKLTGITFHAAIIAGCHRLIRHKQILNDINVFPVADGDTGD
                     NLSSTGMAIIEFSSAKTKLRGTLESIADASIIGARGNSGIIFSQFFNLLAKHLPLKEE
                     LYFNDFSKIMVQVGKEIATVVTNPVQGTMITLIQKWATVCEIKQPDSLLAQAMLELLP
                     LLSVEVEQTKNSLNILQKANVVDAGALGFYYFVEGFTQFLQQSHTTDTANTHSPLSIT
                     EHPQLTCTTTEYRYCTEAVLKSASIDKNKLLHFLKEQGECASVTGNDRITRFHVHTNN
                     PREIFTRLYKDYTIQYPKVDDMQRQHELQFGKKYKIGLVTDSSADLPQDLIDEYQIHQ
                     IPLNIHVDEHHLLDKHCFEANYFYTQLNSFRTYPKTSCMNPNLIEEKLAKIASHYDHV
                     LVLSISSKMSGTYEAFLTATKKYTNITVLDTKTNSGAHGLLLHYAGQLIASGNDIDTI
                     AKLLGKAIKNTYIFVMVNQFESMIRSGRVNKISGRIAQWVNIKPLVSIDSAGQGIMVG
                     KCFSASRARNKLINFLQEKKDEAQLILEDYCIIHAGDKNQALHLAEMTTQAFNKPPTY
                     IEPVSLAIGLHAGQGCIAIAGRLRERNDY"
     gene            28046..28744
                     /locus_tag="ACF2SP_000097"
     CDS             28046..28744
                     /locus_tag="ACF2SP_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2357913.1"
                     /translation="MARYLVIAASSAIGQSVTTLLKNRGDTVFTTARNNHKISPDFQL
                     EATHFDAVLDVFHQTGPLDGVVNCAGSLLLKNAHNTSFDEFQAAINASLTTSFATIRA
                     AGLTMNQGGSVVLISSAAALVGLANHEAIAAAKAGIIGLAQSAAATYASNNLRVNVVA
                     PGMVNSPLTSSLLSNQLVLNASKAMHALGRIGVPEDIAHAIVFLLNPDNSWITGQVIA
                     VDGGLSRVRPKMKI"
     gene            28761..30299
                     /locus_tag="ACF2SP_000098"
     CDS             28761..30299
                     /locus_tag="ACF2SP_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HFD2357914.1"
                     /translation="MTTLCFILGDQLNETIASLAAINKQNDIVFLCEVAEETSYVPHH
                     PKKIAFLFSAMRHFAQQLKKKGYRVRYTAFDDPRNQGSFAKELLRAIEEEQVSAISLT
                     EPGEWRVLKIFHDLKSQLTIPVTLHEDNRFLCKINEFRNWAKEKKQLRMEFFYRMMRQ
                     KYHLLVDNDGKPVGGSWNYDAQNRKNANHISSFPKRLEHARDVITDEVLGLVSAHFAK
                     HFGQCYPFNLAVTREQALEEADYFMEHFLCNFGAYQDAMLKDEISLYHSKLSSYLNAG
                     LLLPLELCQMAEQAYFSKQMPLNSVEGFIRQILGWREYVRGIYWLFMPDYGEMNYFNA
                     CRPLPSFFWGEETGMFCIKEVVRQTSTEAYSHHIQRLMITGNFALLAGLDPKQVCEWY
                     LAVYADAYEWVELPNTLGMALYADGGLMASKPYAASGKYIHRMSNFCQSCYYNPNDLL
                     GEKACPFNALYWNFLHQNQEKLKKNPRLRYAYMNWQKMDFDKQKKTIHKAQRILADLD
                     SANL"
     gene            complement(30304..30717)
                     /locus_tag="ACF2SP_000099"
     CDS             complement(30304..30717)
                     /locus_tag="ACF2SP_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HFD2357915.1"
                     /translation="MNGWTLKVFAIAFFIFIVTDMIWLGFIARNLYFDQYQQWLRLSE
                     GQLKPIWWSALLVYLLFALSIIVFVIPLAKTSLPYAALYGALLGAIIYGIYDFTCLAI
                     FKDFPVGMAFLDWLWGIVLCSWSSIATIYVASYIK"
     gene            31081..33966
                     /gene="legK4"
                     /locus_tag="ACF2SP_000100"
     CDS             31081..33966
                     /gene="legK4"
                     /locus_tag="ACF2SP_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector kinase LegK4"
                     /protein_id="HFD2357916.1"
                     /translation="MKLLRFHELKSLPGMDEKALELLIKVLGNKGIRKLIKSANGKPI
                     SREIMIHEFGIDCQILFITTGASLKPIIVPTENKISGGGKSYCEQFKVYALDDGKTYF
                     LKSVKIDAESVTEFTNEKDTLSKLGRLVGTFFNEQTQVHYILTTFIKGIDLSRYKNAL
                     PLSVNLKHFWEVLGIMISVCHQVKQFHELGLIHRDLKPGNIMLDADMQCHLVDFGSSS
                     SDKEPKPVSWGTASYLAPELNAQEDFIAFSQASDLFALAYSLDELFNPFRQVKFAKVD
                     IGIKNKHLVLLHAEIEACITGLMSNETSARTLYFSRILQLQRVPESFKSRPEAFTYMI
                     MLLTQWKSCYEIPELKKELDEIIAEIKVAYENHEQDVLKITSMLEQLSKADGLLNSHK
                     SLLSVLIKSLANVQQQELGQDDILPRRFESDVVSRLIKTPTAKMMAAIKQVSDAIVKI
                     LEQYEHSPTESFEYAVLQDFLEQMVQSDILFGAPKEKIEISDIIKILKANRPEDFVQI
                     QHISFKFAQVALRDLALHDLPKHEPEGAFLEILQKYQAKYAPEETSLFELYHHMAGVR
                     DHNPSKVKKGEGYRFRAFICDELFYGDEIFSTGDNRGREGKPNQRLQTNQVGLMKFEH
                     SVHTKGLLTLSGQSWYADCKTQLPNYDSIHYLSALKTDCPYITGPSGMTSLFMNMMFL
                     LLNPKDQDVILSYSLGVMTYVVGAGYHSIKEILIPMVKCVGIVPDYPQHKGTEYLTAS
                     PLYNHYFKAIEEFDKEFAEVHEKIWQEYLTYFRMTYMPVCMRDHCLPHQVPSMEDISS
                     EVKEMLEIVSKSVKNCLEEHQVKRTGDMGLFLSTPCGQTKALEVLSHVCQQQVGLTAI
                     FRQIQAYFKGTFETEEGIIINKEMQLKFIAHFFDVLKESPTLLSQFNLTLRIENTIGV
                     HQCEIGSFRRKELFLELKKTFIAANEEQMVANHEPRKETRNVIILPY"
     gene            33985..35955
                     /locus_tag="ACF2SP_000101"
     CDS             33985..35955
                     /locus_tag="ACF2SP_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357917.1"
                     /translation="MLSKEKINPSLKNTKRQGSDNLAAMADPTSLKNTGDYRCFNSKP
                     EAYIYFIFLLNQWQTNYMDDKREAIAKAIDSIKMAYENHEQDTLVIHKTLHGLSHLLE
                     QEHADELNALCLVPIKDNQSNANNVLPRRFESELACRLIESPTKSMMQAIDLVSQAII
                     NLIEEYSKLDSKSSERAALNFFLQELEGSDIEFGAFKKRPSLEEVIAILKENNPKNFI
                     QIQFISFMFARSVIGPFPLRNLPPFKPTGLFAQVLDKYLQDHPDLKEEEEGFSYQLMA
                     AKNEAEEFRTFITKKLFYQDELYTQGENRGRQGPRKIHLKTKQLGLMKLDHSAHSRGL
                     AVIPEQTWCADAQAQEANQNSIYHKTALRNDCPYVTGPSGMTSVYMNMMMLLLNPQNI
                     ELIQAYSLGIIAYIVGAGFHSVNEILIPLVKCINLLPDYPSYEGLEYLTHPPLYHEYF
                     KQIAQFDSDFIQLRDKVWAYYLNYFKSCYMPLCMRDFCGAEQLANCPDPAPEVKILCD
                     IVLKSIEDCINFHNLNRHLDSRFLGTTTADIDVLNQLKEACASQSSISGIMRLLQKYF
                     SGENIARPTAMHKFETRSYIRFFLDALKDNPQIISHINEKMKLDPPLIISTKTEIFSD
                     DFRIECFNLHRILKREHQVSENCNSTSQFLKL"
     gene            35999..36622
                     /gene="ravG"
                     /locus_tag="ACF2SP_000102"
     CDS             35999..36622
                     /gene="ravG"
                     /locus_tag="ACF2SP_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavG"
                     /protein_id="HFD2357918.1"
                     /translation="MKIIYPGRVICDMKNAAIIKNRIFLNLDKPVKRFLASDKADTPM
                     TAEFYAEKDYEQLFLDFLSQATGSYDEQISMLIAELDSGADRVAQKLMSALYSPWQKN
                     LFPKAIKTIANKSEEYPLMSDLLIKFCQQHVGSVDAVDDFGETALAKILKKDQQRKSP
                     LLFLVKHGAKHCQLTSALQDSLIVNNSDIYNVAEDNTMDWISNCPQP"
     gene            complement(36764..37210)
                     /locus_tag="ACF2SP_000103"
     CDS             complement(36764..37210)
                     /locus_tag="ACF2SP_000103"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFD2357919.1"
                     /translation="MPGSLPLKQLVFFLGMLTQANIPSWYEELNKSGLTPPGPVFSIV
                     WSILYALLAIVAWVLWCSRNESEMRSVFYLYSVQMLMNWAWTPLFFVLHWTGLGFLWI
                     LVMAGLTGLLILSLKNKKESIAILLLPYLVWLLFAAYLNGMIWLLN"
     gene            complement(37271..38686)
                     /locus_tag="ACF2SP_000104"
     CDS             complement(37271..38686)
                     /locus_tag="ACF2SP_000104"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFD2357920.1"
                     /translation="MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLG
                     QAQAWWLHHSLIALGKSLNQQGLSLVLRKGDPQEIILELVAQYGVESVYWNRCYEPQV
                     IARDKKIKLALGEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
                     PFMLTKRPRGIEITSDDLGKWKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHHLSG
                     YKRERDFPAKNATSRLSPHLHFGEISPWVILRALELAKLEQTCDLASVEHFLSELGWR
                     EFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATG
                     YMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPY
                     FRIFNPVLQSQKFDPDGIYIRQWIPELAQLNSQSIHQPWTDADANSIFLGKKYPKPII
                     DHQEARTKALSYYQQIKKGMD"
     gene            complement(38781..39488)
                     /locus_tag="ACF2SP_000105"
     CDS             complement(38781..39488)
                     /locus_tag="ACF2SP_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFD2357921.1"
                     /translation="MPSSILSESMIQAFFWSLITIGMYFITQKLYRRWPFWWLMPLAL
                     TPILVASLVLLLHVNYQDYFRGTKWLVLLIGPATVAFAIPIYEKRALIRQHWPVLLIG
                     VIVGSFTAIVSSWGLAILLGLDDNLRLSLLPRSISTPFAIEISKGIGAAPDLTAIFVI
                     VTGILGAILGEFILSRLPFNSALARGALLGVGAHAAGTAQAHKIGNTEGAIASLAMIL
                     VGLFNILLLPLISHLLK"
     gene            complement(39466..39855)
                     /locus_tag="ACF2SP_000106"
     CDS             complement(39466..39855)
                     /locus_tag="ACF2SP_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFD2357922.1"
                     /translation="MANKIQQNSFFQIVLVGLFWLASELLLKLVKLPLSGGILGLGIV
                     LLLLATNCLKLNRIRRGAELLLANMLLFFIPGVLAILEHQEFIGLLGLKILFIILLST
                     ITVMLVTALVVDYCYRWRTHYAKQHSL"
     gene            39961..40845
                     /locus_tag="ACF2SP_000107"
     CDS             39961..40845
                     /locus_tag="ACF2SP_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2357923.1"
                     /translation="MEFKLLRSFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELG
                     ITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGS
                     STLFAPLFVLYRQHYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
                     SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVA
                     QSSQIDFMLELVAAGLGVTFLPRMIATQRHKTQIRSLLLNGSEFQWNMAMTWRRNSYL
                     SEAAKAWLALVREVHVDN"
     gene            40968..41180
                     /gene="yacG"
                     /locus_tag="ACF2SP_000108"
     CDS             40968..41180
                     /gene="yacG"
                     /locus_tag="ACF2SP_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFD2357924.1"
                     /translation="MNNQQKIKCPICGKQNTWSPDNQFRPFCSERCKLIDLGEWASES
                     RKIPGSSIDPESIVTSNNKQDNEDEQ"
     gene            complement(41266..42345)
                     /locus_tag="ACF2SP_000109"
     CDS             complement(41266..42345)
                     /locus_tag="ACF2SP_000109"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFD2357925.1"
                     /translation="MKLGILGGGQLARMLALAAYPLGINTVCLDPSPDACAGQLTRLI
                     VSDFNDELALKQFLSEVDCVTIETENIPFSCAELAAQIKPFYPSTQALKITQDRLHEK
                     NFFRTLNIPVPEFLPVESEDELIQAISVIGIPAVLKTRRFGYDGKGQYVLRTQADISK
                     SWGLLGGQPLILEKLISFESEFSLIAVRNKSGETSFYPLIKNHHRKGILHFSEAPVHH
                     DALQQEAQTHAFKILEALNYVGVLTIEFFYQGNQLIANEIAPRVHNSGHWTIEGAHTS
                     QFENHLRALFDLPLGSTEVSGSCFLLNCIGNLLPLEPCLSIPGVHYHAYGKAPRPDRK
                     VGHVTLIDTHLERYAKNKNLLLDIS"
     gene            complement(42342..42842)
                     /gene="purE"
                     /locus_tag="ACF2SP_000110"
     CDS             complement(42342..42842)
                     /gene="purE"
                     /locus_tag="ACF2SP_000110"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFD2357926.1"
                     /translation="MKPILIGLIMGSQSDWQTLIHAAQTLDALNIGYEAEIVSAHRTP
                     DKLFQYAEQAEKRGLEVIIAGAGGAAHLPGMVAAKTSLPVLGVPVMSQTLNGVDSLLS
                     IVQMPAGIPVGTLSIGKAGAINSALFAAAILANKYPDIRAALKRYREQQTQKVLDNPN
                     PKEEKS"
     gene            complement(42902..43741)
                     /locus_tag="ACF2SP_000111"
     CDS             complement(42902..43741)
                     /locus_tag="ACF2SP_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6671 family protein"
                     /protein_id="HFD2357927.1"
                     /translation="MHYQNQRVLLASKHQKEKAIQPPFEEKLGCRIHVPADYDTDQFG
                     TFTGEIPRKASAYETLILKAKAAAKQFGYRYVISSEGSFGPHPQLYFCPADTELMAFI
                     DLDNDLIIAEHEVSTETNYSHIDISATDDYEAFLNKAKFPSHGIIVRALNSEENYIQK
                     GICEREQLKTAIQKAFTYSTTIRLETDMRAMMNPLRMNVINKLAVKLVHRIQQYCPQC
                     HTPGFGKISAEGHLACISCGTPTELYLRKVLNCLKCEFKTYQARDDGLEFADQRYCPY
                     CNP"
     gene            complement(43766..44119)
                     /locus_tag="ACF2SP_000112"
     CDS             complement(43766..44119)
                     /locus_tag="ACF2SP_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFD2357928.1"
                     /translation="MNIIKVSEKKEMNISLHPMKKIEVIVSGDNENMVATMMEKANIS
                     GFTLLRNVSGKGHHGFHEGKILFNDKASFVMFIAVAPEEAIANIALGMKTLFQKNSGV
                     LFVSDVSVARVDYFS"
     gene            complement(44133..46421)
                     /locus_tag="ACF2SP_000113"
     CDS             complement(44133..46421)
                     /locus_tag="ACF2SP_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HFD2357929.1"
                     /translation="MSSHITLIKTNTNNENKMTQFNYKVDNDVMIIRAMVNNVAKQMT
                     PVWPLEKFIACNALHGFESMSFEEAVIQNQTAKKGTPFNEKLERVNWHMIKWCGSFLD
                     IGQGTLEMPHRDKGLYFGFLKLAPFDSALHQNSKSIKSWLSNLPEMPEQAIRLCLDKL
                     GVLNQKQEDFLQSTLSHLPGWAGYIKWISEWKNRNGKEENPVSLVDFIAVRLVITCIL
                     WPEASQEEKKKKKDSADTKQLIQNIKNKEDDYRQLLLKKLLPELSKAHIKENRANAQM
                     VFCIDVRSEPFRRCIEKLGHYETLGFAGFFGLPVSIKDYDGETIKDSCPVLLKPRFNI
                     HEKAIAANEHCLEHHEKGKEFKNILNRVYQQLKYNFSTPFALVESLGIWCGITMFLKS
                     CSPIFARRLTKDLNEMICPSIQTQPVFELDLLEKEVGISLQEQIAYAEMALRLMGLTD
                     NFAKLVIFCGHGSSTQNNPYASALDCGACGGNQGGKNAQLLASILNKIIVRRALAENG
                     INIPQDTLFYGAQHDTTTDEVEIYHSNVSQFIHQDILDQLRTDLNMAKHNNNLERINY
                     LNSIDCAEKDIARRSTDWSETRPEWGLARNAAFIVAPRQLTKNINLEGRCFLHSYDWS
                     QDKDGALLETILTAPMVVAQWINTQYLFSTIDNVAYGSGSKITHNVAGKIGVMQGNAS
                     DLMHGLPLQSVMSHDDKSFHEPQRLLTIVYAPREIISELVEKHDVLKTLFFNEWVHLV
                     AIDPRSHLFYKLEKTNNWSVIQ"
     gene            complement(46436..47956)
                     /locus_tag="ACF2SP_000114"
     CDS             complement(46436..47956)
                     /locus_tag="ACF2SP_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFD2357930.1"
                     /translation="MDKLLNGLSISLMLSPLIAMLFYSLLGKTRNIQATYYGTTVIGF
                     GFGLSLLALIVSANTVNPNSYFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCY
                     RRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
                     KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSPAIAVALGFIILTAMTQSAI
                     WPFHRWLMSSLNSPTPVSALMHAGLVNGGGFLIVKFAPLFVAKANLLTVLFIFGALTA
                     LLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNS
                     GSAVQQKKYQNKSRPEKLTNLLTAFLGGLGGMYGFALITGKSILSLQASTFLLGFAFI
                     TGAQLTLGLIAYEKHFKKVILVLALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSSL
                     QVLTLIIFFSLWLVFSMGLFKKIRETRLWDWFYMYMFNASTPHPKTITANRHTYYY"
     gene            48058..48909
                     /locus_tag="ACF2SP_000115"
     CDS             48058..48909
                     /locus_tag="ACF2SP_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2357931.1"
                     /translation="MSIDTITLQCFLAVAETQSFTKAAQRVGRTQSAISQQIAKLENL
                     IEKPLINRGRELSLTTDGEIFLGYAKRIYELHRESLDRFKAPELQGEIRFGLPEDFAS
                     IILSDVLVEFSRLHPRVLLNVECDLTLNLIERFNEGKFDLILIKTTQQQIGTECIEVC
                     IEPVEWIGKKELLPLLDKPGPIPLVLSPTPCVYRGNVIDALDKAHINWRLVYSSASYA
                     GKMAAVRAGLGITAIQRSMIPNYLERLDYDFLPILNDVHVSLLKNSESNKAIESLEFF
                     ILQKLKY"
     gene            48951..50540
                     /locus_tag="ACF2SP_000116"
     CDS             48951..50540
                     /locus_tag="ACF2SP_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFD2357932.1"
                     /translation="MNAKPINSIIDVLDRQTISSIAMISVIISLLSLSIPIAAQTLVN
                     LIAFGKLLQPVITLSLMVLILMMALGALHIWQIVIIEVIQQKLMVKVSLSLTRHFTHM
                     SLDNFSTHHGPELVNRFFEIVTIKKSLASLLLYGINLGLQLFFGLLLILIYHPLFLLF
                     DIFIILGILLIIFIPYKKALDSAKKECSEKHVVGAWLEEILINRYLFRFSLYHRYVTQ
                     QTDKKLVAFLKARNTHFKQLIKHQIGFYTLSALASSLLLGLGGYLVINNQLSLGQLVA
                     AEIVLGALIYSFKRFGALLENYYDLVASENKIDAVLNLSLEQVKEDEHGELLTPINSI
                     QLEIDSKSKALITVDKPLLVFSEKSDLCQLFVEQLFGFQDPASLHVLINEIPCARKNL
                     ISLRRFSLLIAEPQWFTGSIYDNLVLNHRNVPNKIIIEQLKNVGLADKIIQLPEGLRT
                     ILYEWQDVFTSVELTKLMLVRAILLQPELIVIDCAFDILNHQEVNEMMELVLSLKNTL
                     LVIVSQRSDFNYLNNRLVISS"
     gene            50537..51523
                     /locus_tag="ACF2SP_000117"
     CDS             50537..51523
                     /locus_tag="ACF2SP_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFD2357933.1"
                     /translation="MKLHAYSMISKLPKPQKIAKIIFIVFVAIILFLSFTPWQQFALG
                     NGKVIAFSPTERQHTVNSPISGRIKKWYVDEGMRVKPGDPIVDITDNDPELLSRLEVE
                     KKAILLRIEAAEQAIAAGKANVDRQKKLYEQGINSRRQYELAQIEYAKYQNELAQANI
                     DKVNVDVRIARQKTQIIKAHVAGIIFRRLTGQESVVVNAGDVLAQILPETESRAVELW
                     VDGNDIPFVRLNQKARLQFEGWPAIQFRGWPEIAVGTFGGTVSFIDPTDNGFGLFRVV
                     IVPNEPWPSVRFLRQGVRVHGWVQLGQVPLWYELWRQYNGFPPESDRENKAS"
     gene            51520..52917
                     /locus_tag="ACF2SP_000118"
     CDS             51520..52917
                     /locus_tag="ACF2SP_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2357934.1"
                     /translation="MICFPSGKNRLIVLCLLLILSFKCFPIPGKPLLLDAVLKSVDRS
                     YPQIVIARLEIRKAEGDYVNALGKFDPSFDVNPRFQPVGGYINNYADNLFNVPTLYNG
                     LKLFGGYRIGRGDWPIYYQNYLTNSGGEYRAGLSLPLLRDRRIDKERTELFTREETIR
                     MKQQDAAATKIKIYQETIKAYWQWVEAGFQLKTFRHLLNLAKERQNAIIQQAQQGDLP
                     RLAIAENLQLIVQREQLVNQGEMLLAQAGINLSLYYRDANGKPQKPDESQLPATFPAQ
                     PIRVSNSISQLQRHPVLRKLENYSKIVKLKQNLARNELLPNLDATAYTFKQYGTGGYP
                     LLIPQAAMIGVNFKFPIFQREARGKLISTTSELQQVKTEMKFTYEQLNNELSNLLVAI
                     RIYQQQVVLLNKELNLARQVEKGETKRFYEGDSTLFLVNQREQTTAQVKLNWINAEVN
                     LQEATALARFFSSTQ"
     gene            53100..54206
                     /locus_tag="ACF2SP_000119"
     CDS             53100..54206
                     /locus_tag="ACF2SP_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2357935.1"
                     /translation="MSYSIDNPGGGDCGFYALAIGLISIIQNEYKLHGKSKTYDRWKQ
                     VEGIYGVRQQDILNVDLTKLHDSPYTYKSELLFLLQMSLRNIAVIVNKEDLIKRIIEE
                     GRSKDQTKIEGSNAYCKFMELVQLYLYRRDSLARISQFNELALSPEIVQTARDIAKIL
                     RPILRNQPFHQVQKIENAFVKEVLLNDVLSGNTINSNSIILKNLEKIKEQGRWATHDD
                     LKEIADLLKINLNVVGQPNGKPILEYPTVTLKNLSNTHWITQVESLQGPRQYSTPRKR
                     AFGYETVSDRAVPLKEEQTLDRAFRTKTQSERTKRYHQHFETLFDTLSPYGFFTYEQN
                     KRNTQVSGKVQTRLESDESLAAQLQEAELRRILY"
     gene            complement(54377..55942)
                     /locus_tag="ACF2SP_000120"
     CDS             complement(54377..55942)
                     /locus_tag="ACF2SP_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2357936.1"
                     /translation="MLKRDISTLNILVASAGGMIGSGWLFSPFISAQMAGSNSLISWV
                     IAAIFMLFIALPLCELGTMFPVSGGMSNYPTYTHGREVGFLFAWTSWLSYVVMTPIEI
                     QAILQYSSHFFPILIDNHSESLTLSNYGYLAAIGIMFFVVILNSYGIKFLAECNKYAS
                     IIKFVLPSIAIISLLKQSPSLANVNINLSSKEHWVEIFTALSAGGVAFAFTGFQNGLI
                     LAGEVKNPQRNIPIAILGAVLVGFILYFMLQFSFIAAVPQKYLAQGWHALSYPGDEGP
                     LVGLTLLLGLGIVATLLLFDAAFSPFGTTLVYTAATSRILYGMALNQHLPKFFLKVNR
                     HNIPYVTLYANLLVGSFAFLPFPGWQKLVAFLSSASILSYGIGPICLLAMRKMHPHMD
                     RPFKLANSIFLSHIAFYICNLMLYWCGFSIIWKLNLALLMGLGIYFFYHRHISMRNGS
                     PLVWFIMYMGSFSIISYLGSFGGIGLLKFPFDLICFLPYSILILYMSQVLATPRHDND
                     MVGNVTELPVYNS"
     gene            complement(56090..56923)
                     /locus_tag="ACF2SP_000121"
     CDS             complement(56090..56923)
                     /locus_tag="ACF2SP_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFD2357937.1"
                     /translation="MFSKALISATYKNGKPGQLTEEHEKAEHHRFKTEYLFKNTEIPK
                     DLYASRLIQHFLIIKAIETKLQNLPKTEQSEISAFFALSYLEHLWRTPAIENDLRQLN
                     INPDEIHKDVITKTTENYLENIEKFTPKILLAHFLLHIAGFMHGGNIIRSKYIEPSNR
                     LTTYQITADQYDFSAAISFLSSGKHSPLALYQDMMKQIDNIVLTGDEYQELLEQCKGI
                     YETMSNIYDDLCDMHTHHLKLPTYSLAAISVSLVALGLILKLLMDFLNPMTNPIANAP
                     R"
     gene            57214..>57414
                     /locus_tag="ACF2SP_000122"
     CDS             57214..>57414
                     /locus_tag="ACF2SP_000122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357938.1"
                     /translation="MPVYSRELNPQELVNQDVKANACLFKPVRCVNDLFINIRLYLTK
                     AQFNEFKIMDFFKKNETKYAAWE"
BASE COUNT        17555 a        10729 c        11630 g        17500 t
ORIGIN      
        1 ttcagtgcgc tgctttattt tgagcctgat aaatcctaaa aataatcgtt ttattgagag
       61 acactgggta taaagaggat atagtactat gtttatcaaa tctccatgta ttgatttaac
      121 caggcactca aaaatatgga tcaatcctga tggagagata cctaagaaaa ttgtagagag
      181 attaaaatgg caaaaagaaa ctcggccgag ggatgcgatt accttatttg taaataaggc
      241 ttgtgaagat aaatccaatt ctgcagtgga atcattaaga gcgtgtggag ttaaaataaa
      301 aataattgaa ttatgtttag agaaaaatga aaaacaagat gatccattta taattgcttg
      361 ttttaataaa gcgttggctc tagctaaaaa agagaaaaat cttgtggatc gagtcagggc
      421 atctgttcgg gcaacaaacg ttttaagact tatgaagtta gtgcaacatg aaggcttgta
      481 ttcagataat gatgttttat ttttaaaatt tgaggctacc agactactgt ctccgtacct
      541 ttttggtcaa tatgaaggag aggtaaatga tgttcatctc tttggtgtgg ctataaatga
      601 ccccctaact acggattact tttatacccg gctagttgaa aaaatgaaaa agccatggga
      661 ggaagaaatt acccctgatg agttcgaacc gccatgtggt ctttatctta ttccagatga
      721 aataatatca aaaatccagt ttggacattt aaaatttgca gagataagag attgtattat
      781 tacaggaagt gatcaatctc accatgatat tacgcgtgca aaaaaattat taaatttgga
      841 agaagatagc ttgctggatg aagctaaaag catagtagcg tcgcaagaga agcagtatag
      901 aatgtgatga tacggatgct cgccatctaa cttttagcca aataaaataa tgcaaaggct
      961 ttattttgtg tatttagtct ttgattgttt gctgaagtgt ttcagaaaag tcaatctcct
     1021 tgacatgttt agtattgtga ttaaagcgat acttattccg aatcaatgag agaaacctca
     1081 attagtacat ggctcttagc cttgctaccc gctctgctgt tgagggatga gttgaaaaga
     1141 ggttcgccag cttttctcca tttaatggat taatgataaa caggtgcgca gtggcagggt
     1201 gagtttctgc ttcatcaaag tattgctcat ggttggcttg atcaagcttt aacaaggcac
     1261 tggccagcca ctgcggattc ccggaaattc gtgctccacc ggcatccgct tcaaactccc
     1321 tggagcgcga tatagccatt tgaattagtc cagccgccag gggagctact atcatcatga
     1381 tcatgccaac cactgggtgg acaccttctt cattatttga actgtggcca aacattgaaa
     1441 gccacatgaa catattggca atcccgctaa tcgtccctgc tattgttgcg cttaccacat
     1501 taattaaagt gtcgcggtgg attacatggg ccaattcatg agctaaaact ccggtgattt
     1561 cttcttgagt gagcctatct aacaatcctg ttgtaacagc gatactggca ttctctggat
     1621 ttctccctgt tgcaaacgcg tttggagtag agttattaat taaataaact ttgggtactg
     1681 aggttcctgc tctatgagca agctctgaaa caatatggta aacaaagtga ttattagaaa
     1741 gaggctcagc attatacatt ttcaaaacaa gcgtatctga ataccagtaa gctgaaaaat
     1801 tcataattcc tgcgaatagt aacgcaacca acattcctgt agaaccgcca agcaatcccc
     1861 caataacaac tagcaatgct gttagagagg ctagcaagat aaacgttttc aggttattta
     1921 tcatttctca tcacctcatt tactctttct atattttata tctcatatat aaggccagat
     1981 gttaaaaatt caacttggag cgttatctct gtttatgtcc atgctttaga gctggagatc
     2041 aagaaaaccc attgagcgta atacgtggtt atttttattt tgagatcaat gtggtatatt
     2101 gttgcgcaaa tttatatgtc attaaacatc ttatgtatag taaatatcca gtatttttcc
     2161 caaataagaa tatagagtct ttctcaggtg tgcggttttc aaatgttgta ggaattccct
     2221 ctgccattga atcattatat agaggtgata ataatcttgc tggcataatt tttcttttgc
     2281 ctactctaat tacaggagtg ttttgtcaaa gttttcctga ggttgtggat atggagcaag
     2341 taagattgca caaattaact aacctatcta atgattttca tatggtgagt atgtcagagg
     2401 atcctcaaat tgctctggat tgggggaaag gatgttttat tacaatcgac cctgttttat
     2461 tttctgatta tattgtagat gttcatgcta cttttcgtaa aaatcagctc aattttcctg
     2521 accgaatgga gcaagaaaga gagcacatag ctttagctgt acctttttgc agcataaaaa
     2581 aaattacaat tgacaataaa gaattagcta acccttttta tctcagtatc ccccaagata
     2641 atcatgaagc aagaatggag tttaatacgc tctacagcga attaatttca cttttaagaa
     2701 aaaaatacac tcaggaagtc gatggggaag aagagaaagt cgcattacgt gcttacgcca
     2761 tacgttattt agacttttat tctaaattcg gcagttgtaa caacccattt aataaaagcc
     2821 ttgctcagtt ggttgaactc tatccggaat ttatgagcaa ttttttgcaa tcaagtcatc
     2881 tttcatcaaa aacaggtctg atgaaagata ttgttctgaa ctcatcagat aatttattta
     2941 aagaacatcc ttatactcaa tcgattgatg cttcttacat ccatagagtt aaagagagca
     3001 ctacctgtta tgaagatgac tgggcaaaac cggtttatga ttagtaataa aaaagatgct
     3061 gattttcctt gaattcgttg aagatgacta ctaacgaggt gagggcattt gatctatgtt
     3121 accttctgta ttatccaaag tatctgaaaa cacggaatct gtttcatctg tatcacccaa
     3181 aaaatcaaaa tctacaggcg taccgatgcg ttctggttcc agttgtttaa tgatgttagc
     3241 taaataaatt gtggttttgt gattaacgaa cgatgataca gcaatgactt ttttattaag
     3301 tatcaggtga taatattcat gaaccccatc gttacctttt tgaataacaa tagatggatc
     3361 agcgggtgtg ataaaataag tattgccggt ttttttagat gtaatgcgtg aacccagttt
     3421 ttcaaataaa caatccatgc cttcttcagg aaaaagagga aattcctctt tggtatctga
     3481 agtggtaaaa aaggaagaat caaaaaaaga tgattttttc acaaagaagt gaccattaaa
     3541 cataagtatg ctcaaaagta tatcatgata aacgatttgg tggtatttga attgctaacc
     3601 aactccattg gttagcaatg gcagtattat ttacatcgta catgtttctt ttctttcttg
     3661 aatagtaaca gtattcactt cagagtttga tgattgtggt tcaaacaaac caaaattgga
     3721 aagaagcgat gttgttgagg cagtttgtgt gcgttagctt ttcttaaggt ggagtgacat
     3781 tttgaaatga ggcagtcgca actggaatgc aaacaagtgc atgtgcattg tttttaataa
     3841 tcttcctccg tctttttcga ttaatcacaa aggacttatt tactttttta ttctaaccat
     3901 ttagtttacg agcctggtat ttggaaatgg acttttaaaa ggactaaaaa ataatgaata
     3961 ttgcgataat tggtggtgga acaggattaa ctactgcttg ggctttggaa aatcaacgaa
     4021 aatttcatgt gactgtattt gaggaaaatg atcgattagg cgggcatatt cacagtatta
     4081 agattaacaa tgttatcctt gaagggggtg cagaatttat aggaccacct gccttgtatc
     4141 ccaatgtgca tcgattgttt caatctctgg ggatggcttt agatcaattt gagttaaata
     4201 tggattttga caatctaaaa caaagggatc atattgtttt accgcctttg tatcatacct
     4261 ctagtggaaa gtccgaggac gtttcgtcga ttctatacac attgtcttgt ggtttgtttg
     4321 gcaagagaag gaaacaaaat gaaactcgcg tttcagtgga taccatgttc acagaaatgt
     4381 acaatttgct tgaaatgaac cacttaatta tggatgcgag gaataagtta ctgcatccag
     4441 acaatttaat gacattagaa caatttataa atgagtttaa agagaaatgt gttcatgtcc
     4501 tctcgcctgt tgacaagttt gctgaggagt tcgtgtatcc tttgattgcc tcaggatggg
     4561 gagtttcaat tgaaacaatc aaaaaatttg gcgcccatta tgccatgaac tatctggcag
     4621 caggaatgaa ttggtttgat gcgccagaag gtttgagcag ctatattgat aaaatggtgg
     4681 cgcaatgcac caacactcaa tttaaagtga atactgctat aaaaaaatta ataccaataa
     4741 caattgatgg aaaaataaag tatcaattat tgaaaagaga tgacacattc gtttgtgatt
     4801 ccactggaag cccaatgatt tatgatgatg ttgtaatctc tacaccagct tatgttacga
     4861 atgaactgct gtctgatatt gaaagtaaca cgataaaagt gctaagggaa aaacttgcga
     4921 atgtcactta ttatgatacg acagtagtat ttcatcagga tcccgaatac ctgtctccct
     4981 acagaacagt tgtgcacagc cgttttgatg ggactcaatc agcaaacacc atgtgcaaac
     5041 cctggaaatt taataaagat gaaataccca taatgaaaac ctgggttttg cctgggcaag
     5101 aaatgcctaa aaacgtactg agagaagttc attacaaaca cccggtcatg gataaaaatt
     5161 actacgaagc acagcaagcc ttgcatgcag cacaaggtga ggccgggtta tggcatggcg
     5221 gtattcttgc tggatttaat gattcacatg aaagtggtgt tacagcagca ttacaaattg
     5281 ctgcccactt aaatcagcga gaacattgtt tagataaaaa tgaacgtctc gcgctattcc
     5341 ctcatatact tgactctgtc ctatctccaa aattgtctac agatatgggg gtggaggttg
     5401 caggagtagt cgcctatcct tcaatgccat aaacctgaca tcggttactg gttgacaata
     5461 tctattaaat tgatatcgct ctcatggaca aattgagagc gataattgtg gatctgattt
     5521 taaattttct tgcgacatta gaggccagat ttaaattctg cagatcaagg aaaatgaaca
     5581 gttggcattc aagattcaag ctctggctcc aaaagcgcat tgatttttac tcagattact
     5641 ttatagtgag tttttcttta aacgcttaat tctttgaatg gatttcattt tatgattagg
     5701 aaaaagataa ctcaaatttc attttttatg attttggcat tgattgttgg ggctgtagaa
     5761 gccaagacta ctcgtcccgc ggatgaacaa caacttgctc gctctattta taaacaattt
     5821 atcgaagtgc aatcaggatt tactaccggt tcaacaacgc cgctggttga ggtggctgta
     5881 aaatacttaa agaaagccgg tttttccaac gaggacattt tcgttggcgg agccagtcca
     5941 caaaaagcca atctggttgt acgttatcgc ggtactgggg agaagaaacc cttgttatta
     6001 cttgcgcata cggacgttgt tgaagccaaa gcttccgatt ggagcatgaa tcctttccaa
     6061 ctgactgaaa aagatgggta tttttatggc agaggcactt tggatgataa agcgcaagcg
     6121 gccatttgga tagccaactt aatccaatat aagcaggaag gatttaaacc caaacgcgat
     6181 atcattgttg ccttgacggc tgatgaagaa ggaagcagcc catacaacgg gattagttgg
     6241 ctgattaaaa atcacaagga tttaatcgag gcagatttcg ctcttaatga gggtggatgg
     6301 ggtgatttgg ctaacgggaa aaaaatatca caaaatatcc aggtgagtga aaaatacatt
     6361 gtcaattaca accttgaggt ccgtaataaa ggagggcata gttcgctgcc aaccaaagat
     6421 aatgccattt atcgtcttgc cggggcttta gagcgactgt caaaatttaa ttttccttta
     6481 aaaaccaatg atgtcaccgc ggcctatttt aaacaaatgg cggctattga gacagggcct
     6541 ttgaaaacag aaatggttga agcaagcaag ggctcgaagc aagctatgca acgtctagct
     6601 gatagttcac cgcaatggaa tgctatttta cgaacaacct gtacccctac cttacttgaa
     6661 ggagggcatg caatgaatgc tttaccacaa ttagcggctg ttaccataaa ttgtcgcgtg
     6721 ctcccagaag attcacctga aatggttgag caatctttga agacagctat taacgatcca
     6781 gagattacac taaagcgcat tggcaaattg tccagaggcc caagttctcc acttagcccg
     6841 gaaatactaa aaacaattac tcatctaact caaacttatt ggcctgaagt cccagccatt
     6901 ccgattatgg ttactggcgc aacggatgga cgttatctgc gatcagttgg aattcctacc
     6961 tatggagtga tggggctatt tttagatcgc gatgatttcc gagcccatgg ccgtgatgaa
     7021 cggattagtg tcgagtcatt ttatgaagct caagcattcc tttatgattt ggtgaaacaa
     7081 ctgtcaagca atgtaactta agtatgattt ttccagtcgg gacttcacta aatcgggcgg
     7141 gatattgatt tgaggtccct tgggtcagtt ttgcgcaatt ccccaatgta aaaaaccaat
     7201 gtttattgag atttggaaaa cagatatatg ttgaagggct taaaggtcat tgatctgagt
     7261 acggttcttg cagggccctc tgtaggcacc ttttttgccg agttaggtgc ttcagttgtt
     7321 aaaattgagc atcctgtgca taaggatgtg acaagaacat ggaaactccc aggagaggat
     7381 cctcattctg aaatttcagc ttattttgcc agtgttaatt tcggaaagga ctatttattt
     7441 ttagatctca tgaaggaaga ggattatctc tttttcctta accttattgc agaagcagat
     7501 attttaatta tgaattttaa aaagggagat gacataaagc tacgaataca agccgatcat
     7561 cttctcagta taaacccgcg cctaattatc ggaaaaatca atggctatgg tgaagaaagc
     7621 gagcgcgttg cctatgatct tgttctccag ggtgaaagcg gttttatgtc catcaatggg
     7681 acgcctgaca gtggcccagt taaaatgcct gtcgccctaa tcgatgtcct cgcagctcac
     7741 cacctgaaag aagctatcct tctggctctc tataaccgag aaaagagtgg caaaggcaag
     7801 gttgtctctg tttcacttta tgatgctgct gtcagcagtc ttgtcaatca ggcatctaat
     7861 tacttgatga ctgggcaggt gcctcagcgt atcggatcac tgcatccaaa tatcgccccc
     7921 tatggcgaat tgttccgtac agctgatgct gcgcttatta tcttggctat aggttctgat
     7981 aggcattttg caaaactatg cactgtcctt gggctaaacg atttgccgga taatgatgag
     8041 tttaagcaca atcaaaacag ggtaaggaac cgagagaagc tcgctttatt aattgctgga
     8101 caaacagcga aatggcaaag taacaagctt ctcgaaatgc tgcacaggga aaatgttcca
     8161 gctgggaaaa ttatggatct caaggacgtt ttcgataatg agcgtgcgaa atctctcatt
     8221 cgaaaggaac tcatcggcga tgttcccacg gcgcgtgtca catcggttgt ttttaaatag
     8281 attaggactt acctaaaacc ctaatgtgag cagggtttaa attttgtatg caagaaaaac
     8341 gaatgtttca aatgcaaatt caaactctgc tccaaattgc gattgcataa cggtgcgcaa
     8401 aaaaatggcc tggaatccta agagccacga ttgtggaatg cctaaagaca agcccgtggc
     8461 tcaataattg aattatttac aagcaatgtt tattagaatt tcttttcata caaagattgg
     8521 cgaaataata tcttaaatca gatacaaaga tatccccttc attttccatt acaaatattc
     8581 tttcaggttg caattcaaga taattggatg ccttcagaat ggagtaataa actgatcccg
     8641 cataggagaa aatccatttg tcgaaaaagg gtagaatcat atcaggatcg ttagaataaa
     8701 atcgttgata aatatcggta attaattcct ttctcatgtc aaaattgctt ttaaaataaa
     8761 aattgttcct atcttgacgg taaagcatga atggcgtttt atcagggttg ttttttaagc
     8821 ctgcattaag attcatcgat acaatgatgc ctttggcatt ttcaatttgt aatgttgtag
     8881 tattgaataa attgtatcca ttatattctt tcagatagtt ttttaaataa tccacagcgt
     8941 tatcagtcaa tgggtcgaaa taactctgaa tagagaaatt tgtcgcatac tgttccggat
     9001 catgttcatc gaagccgcag ctgacatagg tgttgtaagt aaacaaatta ggagaaattt
     9061 tttttgaaaa gatttgatct atttcatttt ctacatcgtc acagctaagt tccaatcctg
     9121 ttagatttcc gctaacacga acaatttgag ttggaaaatc aatagttctt tgggtcatct
     9181 ggttaagatt agacatgtcc tgttcattgc tatagtgcct gacgacggta tttttattct
     9241 gcggcaatgg cgaaaaaata aggccatctg cgttgttatc agaattggcg aaagcgagaa
     9301 ggggagataa aagcaaagcg cctaataaat aacctggttt cataacaaac tcctttttaa
     9361 attaattcag aactccattc aaatgattat ccgattagaa tgctgtttaa taattgttct
     9421 aatcggatgg gacagacagt atatcttatc tggtttgcag attagatcct ataactagca
     9481 taaataaaac agatgcgatg atcgcatgtg agcgacaggg aatggcaaat tttttttact
     9541 ttaaaagacc atagataact attaagaagt ataaggtttt gaatggatgg gcaataccaa
     9601 gaagcaagtg aatataatag tttataaaaa gaacacaatg gcttgggtca aaattaattt
     9661 aatattgatt taataattta ctgttagaat ttcgagccat gtgaaaacct gaggaaaaaa
     9721 tatgatatcc caaaaagaag cacttgtttt actcaacaag tatgaaaaac acttaaatga
     9781 gctgcaaaat attattacaa caagttatca aaatctttta cgttgtgatg cgcaaattat
     9841 acccaatgct ctggctgctc tgaaagctat agcaaacaac actcaatttg taccagttgt
     9901 agctacttca ggcatgagca gtataaaagc gttgagttct aatccacaaa tagctcttaa
     9961 agagttgtta gttaatgatt tttttaaaga tcatttccaa aaaatgtgta ttcactatgc
    10021 caaggataaa ccggaattat taacaaattt acaaaatttt cacgcatgcg taagccaaga
    10081 aaaaattatt atttataaag caaaaaatgg agaacttgca ctaaagtttc ctacccaaga
    10141 atggagagac cgatttattc aaaaaatggg gggcgtatct cattttgcat cttctgataa
    10201 attcaatgcc aataaagaat gtccgcaaac ttatcctgaa aatctcacta cgctgtatct
    10261 cagtacctat actgcaaaaa atggggagtt agccgtagtt tttcctaata aggaaatacg
    10321 tcaaaacttt ttaactttac ttagacacgc ctcttcagca acatggtcaa tttatgaagg
    10381 gcaggatgcg gtatatttta atgataaaaa tttgcatacc cccggatata actttggtat
    10441 taatgcacct gcgtgtgttt cacaatcatc taatgctgct catgcggatg ttcgttatgc
    10501 tgcaagggtt ttgggccaag cacttagaaa tccaaataac acttgcttct tcaacagact
    10561 tccagaggaa ttaattaata aaatcataac cttaagtact gacaatgaag tgcttgcggc
    10621 agaagatgca ataagttcag ttcaacctaa cttaggtaag cctcagttaa aataatcagc
    10681 tatttttaaa aatggtgccg gggcttgaat tttatgtgca aagacagcaa atctttgcaa
    10741 tagaaaattc aaaccctgac tcttgatttc ctttaaccag cgctgcagaa aatcaaaatt
    10801 tcaagccggt atcaaactgt gtaatcaatc ctttcctgtg atttgggact tagggtaaca
    10861 aacgatgatc gagtcacagt ttgtcaccct gaatctgaaa gaatgaggat taactgttaa
    10921 tgttggtatt tgccgccttg atgtcgaagc gatcggctgt cattacctta acccaggcat
    10981 taacaaaatc ttgtataaat ttttcttttg cgtcatcggt tgcataggct tctgcaactg
    11041 ctcttaactc agaatttgcg ccgaagatga gatcaaccga ggtcgctttc catttcagct
    11101 tcccagtttt gcgatcataa ccttcataaa tcccctctgt ctcacttgat ttcttccact
    11161 ctgttgacat gcttagcaga ttgacgaaaa agtcattatt tagagttcca ggtttgtcag
    11221 tgaatacacc atattgattt tgtccagtat tcgcgttaag tacacgcatc ccaccaacta
    11281 aaaccgtcat ttgcggaaca cttaatttca acagactcgc tttttccacc aacatttctg
    11341 gtggcgacat attgttgctt ttatcaaagt aatttctaaa cccgtctgct ttaggttcaa
    11401 gaacctcaaa agacttcaca tcggtcattc cttgtgtggc atctgtgcgt cctggcatga
    11461 agggaacaat gatgtcgtag ccagcttgct tggccgcttg ttcgattgcg gcattaccgc
    11521 ctaaaactat caaatccgcg agagatatct ttttcccatc ggcttgagca ttattgaagt
    11581 tattctgaat gctctccaat gtttttaata cttttgccaa ctcctgagga tcattagccg
    11641 gccaatcttt ctggggagac aggcgaattc gagcaccatt tgcccctcca cgcatatcag
    11701 tgccacggaa ggtggatgcc gaggcccaag cagttttgac cagctcggat gtggataatc
    11761 ccgagttgag aattttaccc ttgaggttag caatatcatt ggcatcaact aatttgtaat
    11821 caactggtgg aactggatct tgccaaatca tgatttcttt aggcactaaa gagccgaggt
    11881 agcgtgaacg tggccccatg tctcggtgaa tcagcttaaa ccaggcacga gcaaatgcgt
    11941 catcgaactc tttaggatta tcaagaaaac gcttggcaat tttactgtaa acaggatcaa
    12001 attttagtgc caagtctgtg gtcaacatga ccggcgcatg ccttttacta gggtcaaatg
    12061 catcaggaac cattgacgag gcattcgaat tctcaggcac ccactggatt gctccaccgg
    12121 ggctcttggt tttaacccaa ttgaaattga acaggttttg taagtagtta tggctccagt
    12181 gggtaggtgt tacggtccag gcaccttcta aaccactggt aatcgtatct ttccctttgc
    12241 cgctgccgta actgtttttc caaccaaagc cttgttcttc tatgcccgct gcttccggtg
    12301 ctggccccag atatttgccg gacgcggctc catgtgtttt tccgaatgcg tgcccgcccg
    12361 caattaaagc aactgtttct tcgtcattca tggccatacg cccgaaagtc tcgcgaattt
    12421 tttcggccgc agcaagagga tctggaacgc cgtttggtcc ttccggattc acatagatta
    12481 agcccattac ggtcgcagca agcggttttt cgagttttcc atctttatcc tggcgcttac
    12541 tttccagcca tttgccttca gggccccaat tgatattgat ggcttcccat gcgtcttcac
    12601 gccctccagc aaatccaatg gtcttgaagc ccatagattc catcgcaaca tttccagcaa
    12661 gtaccaacaa atcagcccat gaaatcttgc gcccatattt ctgcttaatt ggccacagta
    12721 aacgtctggc tttgtccaga ttggcattat ccggccagct gttttgcggg gcgaaacgct
    12781 ggaagccacc atttgctcca ccacggccat catagatgcg gtaagttcct gccgcatgcc
    12841 atgacatgcg tatgaataat ggaccatagt taccataatc agcaggccac cagtcttgcg
    12901 aggtagtcat taattttttg agatcttcta tcacggcatt caaatccaag ctattgaatt
    12961 cttcggcata gttgaattgt tcacccatgg gattcgaagt ggcattgggt tgccgcagcg
    13021 ggctaagatc gagcatttta ggccaccagt attgattggt tttcggcttg tcttcttgcg
    13081 cataggcata atttgggaaa acggaaggcg agatggccag agtgaatgct gcaaacagag
    13141 gtattgttcg tttgaacata gaaatgactc ccttttctat acagaaaagg caaagtatac
    13201 tccatgttac ttttaagcag caataacaca tttaactgtt tattcaacaa gataatttaa
    13261 agtacactat atttttggtt acattttttt tgataactta ctgatctgct ctgctacctc
    13321 aggttcatta atgccacgaa taatcacctg atctttggtt ttgatttttg ctacttcaaa
    13381 gtgaatgtcg cctaaggttt tacttaagct tctcaagaaa tccgaagatt ttacctgatg
    13441 tgttttttca actgtttcaa gagatatcag tgcatccacg tgatgatgtc catctgtgcg
    13501 caagtgtgct gaaacttcag gacccagtaa ggacttcaaa atggaagcag tcgatgtttc
    13561 aagagctttt ggatcataat tatagcaatt ggcgcttacc ttattccaaa aatcggtatc
    13621 caattcttta tctgagcttt taaaaatatt aactcccagt gggaaataag tagaaagtcc
    13681 tttttttgta taggatttga ccaattcttt gtaggcttgt gtttcattat ccgggttatg
    13741 gacatggtac cagaggtatt gatcggcatt ttcatcctgt tctttaatca tttgctggaa
    13801 tatgcaatgg ccaaatccta ggacaacgac tactcctcca tcattttctt tcacatgccc
    13861 taaggtatta tcggtaagcg tttgttctct tcttatggaa ttaaggctct tctctttaag
    13921 ctgttctgaa acgggtaagt caattccacc aagtttgaat ccttgttcct tcatcgcctc
    13981 aaaaagctcc aatttggcaa caacgcttct cagtttttca attccttgct catattcagg
    14041 atgttcaaga ggtagctcag ataatttctt gatttgtatc ttgatgagta ttttcatcat
    14101 agaaatattt tcctccaggg atgcgcctgg ttccattcca tcaacgcaaa tggttgtata
    14161 cccagttttc ttcaaatcag gtagttgttt gataagaaaa tcgctcgtag ctctatccgc
    14221 atggctttcg tataacacaa ctggaggaag agcatatttg gttttaggca tacaatgctc
    14281 catgtgaagt atatctttaa gtatagtaga ttaaatttca atgctcatat aatggacaac
    14341 atattggggt gtggttgtct ggagaataac ttttctgatt gaattgcgat ttggagaata
    14401 aggtagtaag aaaatgaatt tcttatttgt tttctgaagg atgcttgttg aaattttgaa
    14461 ctaacttact ttacttgcca atttgtgctg tagttattgg ttgagtttat cttgttcatt
    14521 gttgttatgc agaactcaac cgattaatac tttgggaaag atgaaaacat tagctcatgg
    14581 ttaaatccat tctgttttct tcagactcat cttgttctct aactatttct tctgattttc
    14641 ctttcactgc ctcctgaaat ttttgtttga agctctgtgt ggttgtagtc agtggcccag
    14701 aagtattcgc tgtttttgct ctttgttgtt ctacaagcgc ttgattcgta ttaagaagta
    14761 tcagttccgt ttgaacttgt tgactgaaat gactgaacga tgtaggtgtg gcgatggtgg
    14821 tttgagaagc tttatctacg ttaaacccat ccgttttcat gatatttttt agctcgtcgc
    14881 tgatgccagc tttgactact tgcgttgctg aagtggaaca attaaattta tagaagctgt
    14941 aggcggttgt gctttgcgct aaactggcca ttttttcaag gattcctgct gtatcaaggg
    15001 caaaagtcgg tgcattgtaa tcgtgggttg tgggtattct gatcactgat gagggaggtt
    15061 ctccaacgct cagataccgc tcttcaaaat ccatttgaca taatttgatt tgctcaacca
    15121 ctctctttag ttctttgtta atctcagcaa gacgaacaat ttcagcatct gttaaaccta
    15181 gatctttatt aggttgccgc tgttcaaggg cagatttggc caattcctgt tcataaattt
    15241 ttcctttata aattaactgt tgcaattcag tttgtaatgc cgcttcctta gcttttaatt
    15301 gctgataagc cgggtcattt tcaattgtaa tagtttcttt aatattttga tgggaaaact
    15361 cttttacttt ggaaatcgtt ttttcactaa ttaatattcc cgtaacattg gttttattag
    15421 cggcaggagt attttctccg tataaatgct tctgtatttc aggtttaatt ttgggagctg
    15481 gttcatgacg ccattctgat tccaaatctt ctctgtggct gttaatgtgg tgaccattgg
    15541 tatgtcctgg ccaccagcta aaatagacaa aatagacgac ttgttctttg ggtttataac
    15601 tcccgtcttc ttgtttttct ggaacgattt ctgtgcgttt actgattgaa agaccaggca
    15661 catcctgata ttttttcgct aactcatctc cttttgcatc agctggaaat gttaattcaa
    15721 cggcagcatg gccaacattg ccaccaaata acatggattt aactggtttg gtaaatccca
    15781 accctttgga accatttttc caggtatata tttcaaggct cataataaag tctccacaca
    15841 actaacatgg ttttattatg agctaattga ttagaaatta aattaaaggg ggcttatatg
    15901 tgcagggaat ctcagcatca tacattccta acaggaataa cgctgaatct ttaaagattc
    15961 atcaagactt atgaaaaacg tcaataggag cgaaaacctg atctgataag agggaatatt
    16021 tctcagtata aacaaaatgg attaactatt cttgccaatg ctaatctgta atcccgcaaa
    16081 tgaattgtat ttataaatag ggaggtcttt atttagaatt ttctcgtagt tttaataatt
    16141 tttttcaata agcttctatt ttcaagattt taaactaata tggaaagata aaagggatat
    16201 ttagtgagca gattatgaca atgatgatta caacaggaaa ttcgtttgac gggaaagtaa
    16261 ttaaacaatg tttgggtatt gtgagaggta tagtagtgcg ctctcctacc atcagccagg
    16321 gattgatggg aggcttgaaa tcgatcgtcg gaggaaaaat tggtgcgtac tcgcaaatgt
    16381 gtgagcatgc aagagaagaa gcatttcagc tcatgataga acatgcccag gcactcaatg
    16441 ccaatggcat tattgccatg cgttacgata cgggcgaaat tggtcaggcc ggcacagaag
    16501 ttttatgtta cggaacagca gtgattatct gataaactat tactattcga gcgaatgata
    16561 gattacaaaa ttcaagtcct gtcccccctg ctgcaagcaa ttctctacag cctgctcgtt
    16621 cttttttgcc ttctttccca atagatattt ttgagagaat ttagaatgat accggaattg
    16681 gataatccac tattattaaa ctatatttta tctattagct tttttcatgt gaattaaaaa
    16741 ataacagaat aaaaagcccc aaaagttgac aatgaggtgg atgatgccca tcgatataaa
    16801 taaatattac agtttgattg taaagatgcc ttgtccatcg gaaggagggc caactctaaa
    16861 tcttgctttt ggaaaggaca ctgcaaaaag atggggggta gtttttttac aacgaattaa
    16921 aacccccaat ggattaggga cagtaatagg agaatacaaa gataggttat ggatatggct
    16981 tgatgataat aggggtgttt ctcgctggga agtgaaagat aatctgttca gtatgaaagg
    17041 ttttgaaccg gttaaaaagc catcgatgaa atcatatcct gatgaggtcg aaaaatatat
    17101 tgagaattta ttccaatgtt ctattaatga acttgtaacg tttactcaaa atccttttct
    17161 tttaattttt ggggaagaaa gtaaacaagc aattgaagcc atagaaaaaa attgggggct
    17221 ttggctagag tacacaacca caaacttaaa cacatggtct aattatttta ccaaatatct
    17281 tcaagagcac tcacagaacc cattggagaa aatgcagcaa tacggtgagc ttacttctcg
    17341 agtgactggt attcctaaaa taacaattaa agaatttaat ctctcttatg tcaaagagat
    17401 taaagaagtt gaagagcaga tttttgagtt acagcaacaa aaatcgtcat tacttgaaca
    17461 aaccactgca aaagaagatc aagaacagat taaggggatg ttcaaaaatc tagagcaagt
    17521 actacacgac aaactagagc aacttaataa attagaagaa caaaaccgtc gacaaaaaat
    17581 gttaacctct cagttaaaca aagtcctaac taatcaacaa tttgctgaat ttaaccaaca
    17641 tgcgaaaagg attgaattga ctgtaaactc tttattggca atcaagccgc aattacaaga
    17701 caagcacttt atgaaaacct atcaagccat gttaactctg atggcacaat acttatacga
    17761 acaagacctt attgaaaaac agagtgaagc aaccgataag actggcaaag aagaagcaga
    17821 ggccaatctc tcgcctgatt tatttacagg cttggtattt gctatggtag caggtgattc
    17881 cactatcaaa aaagcctatt atttgcggtt aataaatcgt ttggataaca ataataacgc
    17941 agccaaagaa atgcctgtaa cttttgattt agagatttgg gcagtcagca atcctcaaca
    18001 aatgattaat ttgtgccggt ttttatttaa taactctaaa ttcaagcaat ttgaggaggg
    18061 tttgtcttat aaagagtatc ctttatttat ttttgaaatt gtaaaattaa agatgagctt
    18121 tatacttgcc cttaatgagg tgcagaaaaa caacagcaag ttctctatcc ctcaggaaat
    18181 taaattccat gctcaaaaaa ctatagactt attggttgat ttaatccttg atgggaatga
    18241 agaaatagca atctgggcag agaaacaaat taaaggcaat aaaaatccat ttataaaaaa
    18301 ctacagggtt tatgcggcat tggctgagtt ttatgctacc ccaccccatg ttaatgaaga
    18361 aaaagggttg tttttttaca atgaggggaa aacaagcacg aaagatcctg aagaaaataa
    18421 acaagcaaaa ttagtttcca ttttgtatga agcaattcat ggcagcacag gagaaatacg
    18481 gcgagcagcc gtggaattat taaaccaaaa gcgtgaatac cattacttaa aattagtagg
    18541 ccataaaaat cctgatgcgg cattgctttg tttggactat tgcttggcaa ataatcaact
    18601 agatgaggca aaacaatata ttacccttgc tcgtaaggct gatcgcgcta aagcggctta
    18661 ttttgaagcc ctgttagtag ataaacaaaa aggggacttt atgccctttt tcctagaggc
    18721 aattgagttt ggttttgctc atgcgggtta tgctctgaga aaaatgttgt cggtcttgct
    18781 ggaaaaaaaa caaattgaag aaattaacga acgatacctt aatttattaa ataagccatt
    18841 tgttttgaat atacttactg ttccagtgat taaggatact attatggctg tttacaacag
    18901 tactggagat actcaacaac aggaagaatt tagagaaaaa tggatgttca atttacttgt
    18961 tgtggtgaaa aacactgaaa tagtgcagaa aacagacctg gtagttacta ttataacaga
    19021 gtgccgcgag cttttgtctg atgagggatt gttagtggca ttaagtattt tatcaaaaca
    19081 acgatatttc gatgaaccag gtgatccatt cttgttagat gctattgctc aaattattac
    19141 agagcaagat aaaaaaggtc cgaaacattt tttcaacttg ggcatatacg attttcttaa
    19201 aaataacacg aaattagtag ttcttttgat gaataattct tcaagcaaag agagactagc
    19261 tcaatgtgaa cggtttacca gctttatcaa gaaagaacaa attcgcattg aagagtcgga
    19321 taaagtgaaa aatcagccat ccctcgctaa cctgggccta ttcggtgata ataataattt
    19381 accggaggaa aagggtccct caccatcacc catgggaacc aaaaaaggtt aagccgttgt
    19441 tatttgcatt ctgatgtttc gagatttttt cattcctttc atcagattga tgatggtaaa
    19501 atcggaaaga gtacataatc agctcaaaaa gatcaaatat tatcgttagg ggggtgtttt
    19561 cccactagac cttgcataac ttgtctcttt cattttattg ctgcgttgca aatgtttttt
    19621 tggtgctcat tgagttcatg taatccacct ttcaaaaagt ctgtgtcttg acttaaagca
    19681 taataatcag gtgatgcaaa aaatctattg aggttataag cggaaacgtc aatatttatc
    19741 taatctaatt cattgttaaa taaagataat ttgtgttaga ttaaaaaatc tattgaacat
    19801 cgctggtgta tataaacctt tcttaaatgt cataataggg ggattaatga aaaaaatatt
    19861 gggaagcatt ggtcttgcag tagtcagcat ttcactattt gcgcatgggt ctatggaagt
    19921 tcctatttcc cgaacctatc aatgttataa ggaaggcccg gaaagcccta aatcagcagc
    19981 ttgtaaagcg gctgtccagg taggcggaac ccaacctctg tacaattggc atgaagttaa
    20041 ccaggcagcg gctaatgacc gacatcgtga actgattgca gacgggcaat tatgcgccgg
    20101 gggtagagat ttttttaaag gtttcaatct ggctcgcacc gattggacaa ccacggtagt
    20161 gcatcctgat tctaatggcc gctttcagtt tgtttatgtt gctactgctc ctcataaaac
    20221 caagtatttt aaattttatg tcactaagga cggatatgat ttcaataccc ccttaaaatg
    20281 gtccgatttg gagactagcc cgttttgtac aatcaccagc gtcactttag cgaatggtcg
    20341 ttatcagatg gattgcccat tgccagcgaa taggacaggc aaacgaattt tttatgttat
    20401 ttggcaacgt gaggacagtc cagaggcatt ctattcctgt agtgatgtct ttatagacag
    20461 taccgcacag gcaccaacct ggtttgaatt gcagaatttt tacgggtccg gtgatattgc
    20521 cgccaacact aagatcactt tgcgagtttt taagcatgaa gtagaagttg aaagcactag
    20581 catcactgtc actgaaagca atcgtacagc ggaacaatgg cctcttgctt tgggcgcagc
    20641 ggtcaataac agatcctcac tggtacgtat tggtcaactt gatccgcaaa gtggccaggt
    20701 cgttctggca gccaatcagg ccaataagat ataccttagc gatagcgaca ccagcagcta
    20761 ccatgttctt gttgattttg cggaaccctc cggtaattct gatctgattt acccggatgg
    20821 tattagcacc tataaagcag gaaccattgt tctgggtcgt cttgatcgca aatattatca
    20881 gtgtaagccc tggccttatt ctggttggtg ttcacaagcg ccagcctatt atgaacctgg
    20941 cgtaggtttg gcatggcgag aggcttggat tttactgggt taatgcgtaa atcagggaac
    21001 ccttgaggtt ccctttcttt attagttcaa cgtttgttca agtaattctt atgcgtttgc
    21061 acttatggca aattacatca tgctttaaga acccaaaagt gataattttt attgcagatt
    21121 aaaagaaaat aaccaattaa ctgtattcga ttacatggaa atgttttaca atcatcagaa
    21181 tattcattca atttttagat tccatacttc gagctctttt agcatgtttg cagcatgttg
    21241 gtgtccattt tttcaaggta acatcattgg ctcttgtttt agagtagagt atttttatgc
    21301 agagtgaaaa gtggtaagga attagttact gatcgcgcta tgaaataagc aggaaccaaa
    21361 atgacaatta tgagagtatt tctttcattt cttttgctat tttttaatag ccttagtttt
    21421 gcgcaatctg agcttccaca attgtttctg tttttagggg gagacagagc aagcacattt
    21481 caggagactt tgcaaaatcc ttgcattcgt ggggtacaaa tcatttattc ctggaggcaa
    21541 ttggagccgc aaaaaggggt ttacgatttt tcgaaaattg aggaagattt gaacgacctc
    21601 agtagaatcg ataaaaagct gtttattcaa atacaagacc gttcatttag cccagatgta
    21661 tttcctgtac ctgactacat tcgcatggaa aaagcatatc acgggggtgt ggtacagcag
    21721 tatgatttcc ccggagaagg aaaaccaatt accgcaggat gggtagctcg tacttgggac
    21781 cctgctgtaa gaaagcgctt tcagttatta ttaaaaaaac tggcatcaaa atttgatgga
    21841 aaaatttatg gcattaatct tcctgaaacc gcaatagatc ttgatcctaa tcatctcccc
    21901 gaagatttta cctcagacaa atatttctat tcggtccttg ataatatggg ttatttaaaa
    21961 aaggtctttc aaaaaaccaa agtgattcag tatgtcaatt ttttcccgga tgagtggaat
    22021 aatgaccatc actacatgag tagattattc tcttttgcca ggagccatca cattggttta
    22081 ggcggacctg atgtggtgcc ctataaagaa tcacaaatga aaaacagtta tccttttttc
    22141 cacaaattca agggggagat agaagcagcc atcgctattc aagaaccaga ttatacttat
    22201 accaatccaa agacaggcga tttctatcgt tttgatgact tctattcttt ttcgaaagag
    22261 tatttaggag ctttcatttt attttggaat acggaagaac catttttctc agagcagctt
    22321 ttacctaaat tgaatgacag ttattttcaa tgcgataaag tcaatgataa agcaaataca
    22381 gctgatgcca attaagagga cgggaacccc gttatttcct ttttgaggct tattgctaat
    22441 ttattaagat gggcacaggc ttatatgcta aaatagattc aaataactta tatctttgag
    22501 gtggccaatg cttgtagagt tattatcccg cattcaattc ggttttagca ttggttttca
    22561 tattctattt ccaactttaa accttggcct tgcggttttt cttgttatca tggaagccct
    22621 gtggctgaaa acaaaaaatc ccgtttatct cgaaatatgc aagttctgga ctaagatttt
    22681 tgctttgact tttggtatgg gagttgtctc cggcattgtg ttggcttatc aaattggtac
    22741 caattttgga cccttcatca ctcagtttgg aaacgtcctg ggggctttgt ttgcttatga
    22801 aacgttgacc gcttttttcc tggaagctgg ctttttagga gtcatgcttt ttggttggaa
    22861 aagagttcct ccagcacttc attttatcgc cacccttttg gttaccatag gaacaaccgt
    22921 atccgctttt tggatcatgt cagccaattc atggatgcag gcacccagcg ggtatcaact
    22981 cattgaaggt aaatatgttg tcgatagttg gtgggcggtt gtttttaatc ctaccttcat
    23041 tcccagattc attcatatgc tgttagcttc ttatgtaacg acttgttttg tcatcatggc
    23101 ggtatccgga ttttactttt taaggaaaca atctactgca attgctaaaa aaagtttatc
    23161 tttcagtttg tgggcatcgt tgattttagt tcctttacaa ataggagtgg gtgatgtcgt
    23221 aggtattaat gtgcatcatt accaaccact aaaaacagct gctatggaag gcatatggga
    23281 aactcaaaaa ggagcgccgc ttttgttgtt tgctatcccc tcacagacac agcaaaaaaa
    23341 tttttatgaa gtgggaatac caaaactggc aagtttgatc aatacacatg agtggaacgg
    23401 tgaaatgatc ggtttgagtt ctgttggcaa tgaagagcag ccgcgcatgg cccctgtatt
    23461 tttttccttt cgtattatgg tgggtattgg gctcatcatg ttatttacgg ctatcgctgg
    23521 ttttatcttg cgcttaagag gaactctttt tgaaaattca tggtttcatc ggtggtgcat
    23581 tgtaatggca ccactcggtt ttattgccag tatcgctgga tggttaaccg ctgaacttgg
    23641 acgacaaccc tgggttgtcc atggtctttt aaagacgaga gactctgttt ctgcaatagg
    23701 ggttgaagaa gttattattt cttttgtctt gctcattgtt gcctatggag tggtctttgg
    23761 attttatctc tattatctgc tgaggctcat ctctcatgga cctggaattc ttgaagagga
    23821 tgaagtggaa catcatgctt ttcaatatat gaccgatttc cccaaggaga aaaaataatg
    23881 ttaccgttta ttttcgctac acttcttggg tttatcgttt tgatgtatgt cattttagat
    23941 ggttttgatt tgggaattgg tattttattt cctttcacct caagtgagag cgaacgcgat
    24001 aaaatgatga actcgattgc acctgtttgg gatggcaatg aaacctggct ggtttttgga
    24061 ggagccatgc tttatggtgc atttccacaa gtttacggtt tgcttttgcc catcctttat
    24121 atgcctatca tgcttatgct gattgcattg atatttcgtg gtgtgagttt tgagtttcgt
    24181 tttaaggcca agaggtcaaa aactttgtgg aattggtcgt tttcaatcag ttcggttgcg
    24241 gccacttttt tgcaaggagt tattcttggc gcttttgtac aggggttttc tattaatgaa
    24301 tcttccatga ctatcaatga tccggactgg cttacccctt ttagcctttg taccggtatc
    24361 gctttggtgt gtgggtatgc gcttttgggg gctacctgga tgattatcaa aagcacggga
    24421 agattgcagc gtaaaatggt tcattatgcc agaggcttgt tagtcttggt cagctttttc
    24481 ctgcttttcg ttagtatttg gacacctttg catagtgagg aagtgtttca tcgttggtat
    24541 agttttccga atatgctact actgagcccc ttgcccttga tagccttaat agctatttac
    24601 ctcgtatggc agaacttaag ttccggcagt gaatacaaac cgtttattta cagtatcatt
    24661 atatttttat gctcctatat aggaattgca atcagcgtct acccttacct gattcctcat
    24721 caagtgactt tctgggaggc ggcagcacct gattccacac ttgtttttat cttgataggt
    24781 gtcgctatca tgctgccaat ccttcttgcc tatacccttt atgcttatta cctttttcgg
    24841 ggtaaatcat cagaaaatta tcactagtta ttttctgggc gtcgaattat ttttaagcca
    24901 tttagggaaa aacatggggg tcactttttg atactcgatg taaagattgc ctctggattc
    24961 aatcgaacct tgttcagtca tgggggctgt gatttttgtc atgattaaat acagggtcaa
    25021 tggagaaata atggctatcc atccataagg agaggaaagg gcaaataagg taaaggagca
    25081 ccataccagc cattcataaa aataatttgg atgtctgcag taacgccaaa gtttttgatt
    25141 gcagactttt ccaggatagt ttttcttaaa atcttgcaat tggttgtcgg caactgtttc
    25201 caaaattaag gccgtgagaa atataatcaa tgcgagccca tccaatagat ttagtgttgt
    25261 ttgagtttct gaggaagaaa aataccaggg aatggaaaca aggcaaatca atacgccttg
    25321 caactgaaaa tttaaaaaaa atcccaaagg tttggcaagc ttccaatcgt tgcttaaagc
    25381 aagatagcgc ttgtctattt ttttaaggcg tattcgtgtc caccataaat accctccaag
    25441 cctgattccc caggtgagta atgccaagac gagcagtaaa gagcgcaatg aaaattcttg
    25501 attatttaaa taaataagtc cggcgagtgt taaaccagaa gcccatccca catcaaccac
    25561 cgaaggatta ttggtgtaac ggtaccatag ccaaatcaag cacatatgta aaaagatgat
    25621 ggcgatcaca agtaaaatta tcatcttatt catttcctat gcaggtaatt aaataggtat
    25681 aaactcaaag gcaagaatag agaccaggtt aaccctaaag taaggtaaaa tacaacttca
    25741 ctgccgataa gggttgccgc tccaaattgt acccctagcc aataggcaaa gataatagag
    25801 ggaaacatga ctaaccccca aagaaaatag taagttaacc aggaaccata caaaacaacg
    25861 agattaaatc cgaaacttag ccagagagcg accatccaaa gcggaggaaa atgagatcca
    25921 aagaaattgg ctttaaaata gatcataccg ctataaagcc agatggcatc agtgatcgca
    25981 cctaacaagg ctagtccaat ggaaaaatag agggcatcac gccaggggag ttgattaagt
    26041 atctgtacaa taatctgtag gcccatgatt aacacggtaa agattaatcc cagcaaagga
    26101 tagccataag aggctaggta gatacaaaat atccagcaca gatagtacga cagataatga
    26161 actatatgca caggattaat aatcattacg ctctctcagg cgacctgcga tagcaatgca
    26221 tccttgaccg gcatgtaaac caatcgcaag agatacaggc tctatgtacg tgggtggttt
    26281 attgaatgcc tgggttgtca tctcagcgag atgcaaagcc tggtttttgt cacccgcatg
    26341 aataatgcag taatcctcta aaatgagttg agcctcgtct ttcttttcct gaaggaagtt
    26401 aattaattta ttacgtgctc ttgaggctga aaaacacttg cctaccataa tgccttggcc
    26461 tgctgaatca atggagacta agggttttat attaacccac tgggcgattc gcccggatat
    26521 tttattaacc cgaccggaac gaatcatcga ttcgaattgg ttcaccataa caaaaatata
    26581 agtgtttttt atcgctttgc cgagtaattt ggctatggta tcgatgtcat tccctgaggc
    26641 aattagttgc cccgcataat gcaaaagcaa tccatgcgcc ccagaattcg ttttggtatc
    26701 caatacggtg atattggtgt attttttggt tgcagtaagg aaggcttcat aagtgccact
    26761 cattttgctg gatatcgata gaacaagaac atgatcatag tgtgaggcaa tttttgccag
    26821 tttttcctct ataagattcg gattcataca agaggtttta ggataagttc tgaacgagtt
    26881 aagttgtgta taaaaatagt tggcctcaaa acaatgttta tccagtaaat ggtgctcgtc
    26941 aacatgaata tttaaaggaa tttgatggat ttgatattca tcgattaaat cctgaggcaa
    27001 atcggcgctt gaatcggtga ccaaccctat cttatatttt ttcccaaact gcagttcatg
    27061 ttgcctttgc atgtcatcta ctttagggta ttgaatagtg taatccttat ataaacgcgt
    27121 gaaaatttca cgaggattat tggtatgaac atgaaatctg gtgatgcgat cattgcccgt
    27181 aacagaggcg cactctcctt gttcttttaa aaaatgtaat aatttatttt tatcaataga
    27241 ggcggatttg agtactgctt cagtacagta tcgatattct gtcgtggtac atgtcaactg
    27301 ggggtgctct gtgatagata acggtgagtg ggtgtttgct gtatctgtgg tgtgtgattg
    27361 ctgtaaaaac tgtgtaaacc cttcaacgaa ataataaaat cctagggcac cagcatccac
    27421 aacattggct ttttgcaaaa tatttaacga atttttagtt tgttctactt ctacggagag
    27481 taaaggtagc aattccaaca tagcttgagc tagcaaggag tcgggttgtt ttatctcaca
    27541 cacggtagcc cacttttgaa tgagcgttat catagtgccc tgtacgggat tagttacgac
    27601 tgtggcaatt tctttaccaa cttgaaccat gattttggaa aagtcattaa aatagagctc
    27661 ttctttgagg ggtaaatgct tggctaataa gttaaagaat tgagaaaaaa taatcccgga
    27721 atttccacgt gcaccgataa tacttgcatc agcaatggat tcaagagtac ctcttaattt
    27781 ggtttttgct gatgagaatt caatgattgc cataccagta gaggataaat tatcccccgt
    27841 gtctccatcg gctaccggaa aaacattaat gtcgttgagt atttgtttat ggcgaatcag
    27901 cctatgacag ccggcgatta ttgcagcatg aaatgttata ccagttaact tatccaatgg
    27961 catgggatgt ccgtataatg aatattgaag tttccaagct aatccatcat agtaggtatt
    28021 tttatactaa tgcaagaaga atgatatggc tcgctacctg gtaattgctg caagcagtgc
    28081 tattggacaa tccgtgacca cccttttaaa aaatcgtggt gataccgttt ttaccacagc
    28141 acgaaataat cataagataa gccctgattt tcaactggag gccactcatt ttgatgcggt
    28201 tttagatgtt tttcaccaaa caggacccct tgatggcgta gtcaattgtg cgggttcact
    28261 acttctgaaa aatgcccaca acaccagttt tgatgaattt caagccgcga tcaatgcgtc
    28321 actcactact tcttttgcta ccattcgagc tgctggctta actatgaatc aaggaggttc
    28381 tgttgtattg atatcatcag ccgccgcttt ggtaggtctg gccaatcatg aagccattgc
    28441 ggccgccaaa gcaggtatta ttggtttggc tcaatctgct gcagccactt atgcttcaaa
    28501 caatttaaga gtgaatgtcg tggctcctgg aatggtcaat agtccgctga cctcatcgtt
    28561 attaagcaat cagttagttt tgaatgcatc aaaggcaatg catgctttag ggcgaattgg
    28621 tgtcccggaa gatattgccc acgcaattgt gtttttgctt aatccggaca acagctggat
    28681 cacagggcaa gtgattgctg ttgatggcgg gttaagccgg gttcggccca agatgaaaat
    28741 ttaatgaaac tataattctt atgacgacac tgtgttttat attaggcgat caactcaatg
    28801 aaacaatcgc ctcacttgcg gcaattaata aacagaatga cattgtattt ttatgtgaag
    28861 tggcagaaga aacttcttat gtccctcatc atccgaaaaa aatcgcgttc ttattttcgg
    28921 caatgcgtca ttttgcgcaa caattgaaaa agaaaggtta tcgagtccgc tatacggcat
    28981 tcgatgatcc tcgcaatcaa ggcagttttg ccaaagaatt gcttcgtgcc attgaagagg
    29041 agcaggtgtc agcaataagc cttaccgaac ctggagagtg gcgtgttctg aaaatattcc
    29101 atgatttaaa aagccaattg accattcctg tcactctaca tgaagacaat cggtttttat
    29161 gcaaaattaa tgagttcaga aattgggcta aggaaaaaaa acagctacgg atggagtttt
    29221 tttaccggat gatgcgccaa aaatatcatc ttttggtcga taatgatggg aaaccggttg
    29281 gcggttcatg gaattatgat gctcaaaatc gtaagaatgc caatcacatc agttcattcc
    29341 ccaagcgttt agagcatgcc agggatgtga taactgatga ggttttgggc ttggtttcag
    29401 cgcattttgc caagcacttt ggtcagtgct atccctttaa tttggcagtg acccgcgagc
    29461 aagcattaga agaagccgat tattttatgg aacattttct ttgcaatttc ggtgcgtatc
    29521 aggatgccat gctcaaagat gaaatcagct tatatcattc aaaactgtcg tcttatctaa
    29581 atgctgggct tttattgcct ctggaattgt gtcaaatggc agagcaagcc tatttctcaa
    29641 agcaaatgcc tcttaactcg gtagaaggat tcatacgcca aattttagga tggcgagaat
    29701 atgtgagggg tatttattgg ctttttatgc cggattacgg cgagatgaac tattttaatg
    29761 catgcaggcc gttgccttcc tttttttggg gagaggaaac gggaatgttt tgtattaagg
    29821 aagtggtacg tcagacctcg actgaggcct attcccacca tattcagcgg ttgatgataa
    29881 caggtaattt tgccttgctt gcaggtttag atcccaaaca agtttgtgag tggtatcttg
    29941 ctgtctatgc tgatgcgtat gaatgggtag agttacccaa tacattgggt atggctcttt
    30001 atgcagatgg ggggctgatg gccagtaaac cgtatgccgc tagtgggaaa tacattcacc
    30061 gcatgagtaa tttttgccaa tcctgctatt ataatcccaa tgatcttttg ggtgaaaagg
    30121 catgtccttt taatgcgctc tattggaatt ttcttcacca aaatcaagaa aaattaaaaa
    30181 aaaaccctcg ccttcgttat gcctatatga attggcaaaa aatggatttt gataaacaaa
    30241 agaagactat ccataaagca cagcgaatat tggcagattt ggattctgcc aatctctgat
    30301 gtcttatttg atataacttg ccacatagat tgtcgctatc gaactccagg agcacagtac
    30361 tataccccat agccaatcaa ggaatgccat accgacagga aaatccttaa aaatagcgag
    30421 gcaggtaaaa tcataaattc cataaataat agcaccaaga agcgcgccat ataatgctgc
    30481 ataaggtagc gatgttttag ccagcggtat aacaaagaca ataatactca aggcaaaaag
    30541 cagataaact aataaggcag accaccatat tggctttaat tgcccctctg aaagacgcag
    30601 ccactgttgg tattggtcaa aataaagatt tctggcgatg aacccaagcc atatcatatc
    30661 tgtcacaata aagataaaaa aggcaattgc aaaaacctta agcgtccatc cattcatttt
    30721 attcccttaa tgatgatttt tatccttctg gttttgtatt ttacattcaa ttggattcag
    30781 gtaatagagc ttcatttccc aaacctatca tttaacaaaa agttaatttt atggctaatc
    30841 gagataaatg cttcttacta taaaagggta tcggtaattt cgatacttga atatggtgtt
    30901 tgatatttgc gcaatcattc tcttaatgag ccccatcttg ggagcagcta tttggtatgc
    30961 gagagcatgt ttgggagtta ttattttgct ttgtgtgttg ctcttaattt tttcttaatg
    31021 atcgtttttt ataatgcaaa ggttattttt atccaagttt gtaaatatgt gaggtacctt
    31081 atgaaattgc ttcggtttca tgaattaaaa tcattgccag gcatggacga gaaagcatta
    31141 gaattgttga taaaggtttt aggcaacaaa ggcattagaa aactaataaa atctgccaac
    31201 ggcaagccta tctcacgcga aataatgatt catgagtttg gaatagattg tcaaattctg
    31261 tttattacca ccggggcatc tcttaagcca atcatcgttc ccaccgaaaa caagatttca
    31321 ggaggtggta aatcttattg tgagcaattt aaagtttatg ctttggacga tggaaaaact
    31381 tatttcctaa aatccgttaa gatcgatgct gaaagcgtga cggagtttac caatgagaaa
    31441 gataccttaa gcaaattggg tcgtttggta ggaacttttt ttaatgaaca aactcaggtg
    31501 cattatattc tcaccacttt tataaaaggg attgatttat cccggtataa aaacgcactt
    31561 cctcttagtg ttaatctaaa acatttttgg gaagttctcg gcattatgat ttctgtatgc
    31621 catcaggtga aacaattcca tgagctaggc ttaattcacc gcgatcttaa acctggaaat
    31681 attatgctag atgcggatat gcagtgtcat ttagttgatt tcggcagcag ttcttcggat
    31741 aaagaaccta aaccggtatc atggggaacc gcaagctatt tagcacctga acttaatgca
    31801 caagaggatt ttattgcttt ctctcaggct agcgatcttt ttgctttggc ctattctctg
    31861 gatgaacttt ttaatccatt caggcaggtt aagtttgcga aagtggacat aggaattaaa
    31921 aataagcatc ttgtgctttt gcatgctgaa atcgaggcgt gtattactgg gctcatgtca
    31981 aatgaaacat cagcacgaac actttatttt tctcgcatcc tgcaattaca aagagtccct
    32041 gagagtttca aatcgagacc agaagcattt acctatatga ttatgttgct cactcaatgg
    32101 aaaagctgtt atgaaatacc ggaattaaag aaggagttag atgaaatcat tgcagaaata
    32161 aaagtagctt atgaaaacca tgaacaggat gtcttaaaaa ttacatcaat gcttgagcag
    32221 ttatctaaag cggacggact gcttaattct cataaatctt tgctctctgt actgattaaa
    32281 tcgcttgcta atgtccagca acaagagctc ggacaagacg acatcttgcc gcgtcgtttt
    32341 gaatcagacg ttgtgagccg cctgataaaa actccaacag ctaaaatgat ggcagcgatt
    32401 aaacaagttt cagatgctat cgtaaaaatc ctggaacaat atgaacattc ccctaccgaa
    32461 tcttttgagt atgcggtttt acaagatttt ctcgaacaaa tggttcaatc ggacattctt
    32521 tttggtgccc ccaaagagaa aattgagatt tccgatatta taaaaatact caaggcaaac
    32581 aggccggaag atttcgtaca aattcagcat atttctttta aatttgccca agttgcttta
    32641 cgtgatcttg ctttgcatga cctgccaaaa catgagccag aaggcgcgtt tttagaaata
    32701 ttgcaaaaat atcaagcgaa atatgcacct gaagagacat cactttttga gctctatcat
    32761 cacatggcag gtgttagaga tcacaaccca tcgaaagtaa aaaaagggga gggataccgc
    32821 tttagagcat tcatttgtga tgagcttttt tatggagatg aaatttttag taccggggat
    32881 aatcgtggcc gcgaaggcaa acctaaccaa agactacaaa ccaaccaagt tggcttaatg
    32941 aagttcgaac attcagttca taccaaagga ctactgacgt taagtggtca atcctggtat
    33001 gctgattgta agacccagtt accaaactac gattcaatcc attatctctc agccctgaaa
    33061 acggactgcc cttatattac agggccttcc gggatgactt cattatttat gaatatgatg
    33121 tttcttctgc tcaaccctaa ggaccaagat gtcattctct cttacagcct tggggtaatg
    33181 acttatgtag ttggtgctgg ctaccacagc attaaagaaa ttcttattcc gatggtcaag
    33241 tgcgtaggta ttgtgcctga ttatccacag cataaaggaa cagagtatct tacagcctca
    33301 cctttatata accattactt taaggcgata gaggagtttg ataaagagtt tgctgaggtt
    33361 catgaaaaaa tttggcagga gtaccttacg tatttccgta tgacttatat gcccgtttgt
    33421 atgcgtgatc attgcctgcc acatcaagtc ccttcaatgg aggatataag ttcagaagta
    33481 aaagaaatgc ttgaaattgt ctctaagtct gtgaaaaatt gcttagaaga gcatcaagtt
    33541 aagcgtactg gcgatatggg gttgtttctt tcaacaccat gtgggcaaac gaaggcgctt
    33601 gaggtcttaa gtcatgtctg tcaacaacag gtagggctaa ccgcaatttt tagacagatt
    33661 caggcgtatt ttaaaggaac ttttgagacg gaagagggaa taataataaa taaagaaatg
    33721 caacttaaat ttattgctca tttctttgat gtattgaaag aaagcccaac actgctttca
    33781 caattcaatt tgactttaag gatagaaaat acgattggtg tgcatcagtg cgaaatagga
    33841 agctttaggc gaaaagagct ctttttggag cttaagaaga cgttcatcgc agcaaatgaa
    33901 gagcagatgg ttgcaaatca tgaacccagg aaagaaaccc gcaacgtcat cattttgcca
    33961 tattaacctc aaggaaggtg ttctatgtta agtaaagaaa aaattaaccc ttctctaaaa
    34021 aatacaaaac gccaaggcag tgataacctt gctgccatgg ctgatccgac atcattaaaa
    34081 aacacgggtg actaccgatg ttttaattca aaaccagagg cttacattta ttttattttt
    34141 ttattaaatc aatggcagac caattacatg gacgacaaaa gagaagcgat agctaaagcc
    34201 attgattcaa taaaaatggc ttatgaaaat catgagcaag atactttggt cattcacaaa
    34261 acgttacatg gtttgagtca cttgcttgag caggagcatg ccgatgagtt aaatgcgtta
    34321 tgcttggtgc ctatcaaaga caatcaatct aatgcaaaca atgttctgcc ccggagattt
    34381 gaatcagagc tggcttgccg cttgatagaa tcgccaacaa aaagcatgat gcaagcaatt
    34441 gatctggtat ctcaagctat aatcaattta atcgaagagt atagtaaatt agactcgaaa
    34501 tcttccgaac gagctgcgct gaattttttt ctgcaagagt tagagggttc tgatatagag
    34561 tttggggctt ttaaaaaaag gccatctctg gaagaggtta ttgctatttt aaaggaaaat
    34621 aatccgaaga attttattca aattcaattc atatccttta tgtttgctcg cagtgtaatc
    34681 ggaccatttc cattacgaaa tttgccacca tttaagccaa ctggcctttt tgcccaagtt
    34741 ctggataaat atttacagga tcaccccgac ttgaaagaag aagaggaagg cttttcctat
    34801 cagcttatgg cagctaaaaa tgaagcagaa gaatttagaa ctttcatcac taaaaaatta
    34861 ttttaccagg atgagcttta tacccaagga gaaaacagag ggcgtcaagg acctcgtaaa
    34921 atccacttaa aaaccaagca gttaggattg atgaagctgg atcattcagc gcatagcaga
    34981 ggattagccg ttattcctga acaaacttgg tgtgcggatg ctcaagcaca agaagccaat
    35041 cagaattcga tatatcacaa gactgcactt agaaatgatt gtccctatgt cactggccct
    35101 tctggcatga cctctgtcta tatgaacatg atgatgctgc ttttaaatcc tcaaaatatc
    35161 gaattaatac aagcctacag ccttggaatt atagcatata tcgtcggtgc cggttttcac
    35221 agtgtcaacg aaattcttat tcccctggtg aaatgcatta atcttctacc tgattatcca
    35281 agctacgagg gacttgaata tttaactcat cctcctcttt atcatgaata ttttaagcaa
    35341 attgcacaat ttgactctga ttttattcag ttaagagata aagtatgggc atactatcta
    35401 aattatttta aatcgtgcta tatgccctta tgtatgagag atttctgcgg ggctgaacag
    35461 cttgcaaatt gtcctgaccc cgctcctgaa gtaaaaatat tatgcgatat tgtgcttaaa
    35521 tcaattgagg actgcattaa ttttcataat cttaataggc atctggacag cagattttta
    35581 ggtacgacta ctgcggacat cgatgttctt aatcaattga aggaggcttg cgcgagtcaa
    35641 tcttcgatta gcggtattat gcgattattg caaaaatatt tttcagggga aaatattgct
    35701 cggccaacag cgatgcataa atttgaaact cgctcttata tccgtttttt ccttgatgct
    35761 ttaaaagata acccacaaat catatctcat atcaatgaga aaatgaaatt ggaccctcca
    35821 ttaataatca gtacaaagac tgaaattttt tcagatgatt ttcgaataga gtgctttaat
    35881 ttgcatcgaa ttctaaaaag agaacaccag gtgagtgaaa attgtaacag cactagccag
    35941 tttttaaagt tgtaaaggaa aagggaaata gtataaatat cccttaaatt tcattataat
    36001 gaaaattata tatccaggta gagtaatttg tgatatgaaa aatgcagcaa ttatcaaaaa
    36061 tcgaattttc ttaaacctag ataaacctgt taagcgtttt ctggcgagtg ataaagcaga
    36121 tactcccatg acagcagaat tttatgccga aaaagattat gaacagctct ttttagattt
    36181 tttatcccaa gctactggta gttatgatga gcaaatcagc atgctaattg ctgaattgga
    36241 ttcaggagca gatcgtgttg cgcaaaaatt aatgtcggcc ttgtactcac catggcaaaa
    36301 gaacctcttt cccaaggcaa tcaagaccat tgcgaacaaa tctgaggaat atcccttgat
    36361 gagtgatttg ctaattaaat tttgccagca acatgtcggc tctgttgatg ctgttgatga
    36421 ttttggcgag acagccctag ccaaaatctt aaaaaaagat cagcaaagga agtcccctct
    36481 tttattttta gtcaagcacg gcgcaaaaca ttgccaactc acttctgcat tacaagattc
    36541 tcttattgtt aataattcgg atatttacaa tgtcgcggaa gataatacta tggattggat
    36601 ttccaactgc cctcaaccct aattcttcag agggtaatat attctgttgg ctcaaagatg
    36661 atgggagtta tttagaagtc ctgtacttat ctcgcaaagt aagatttagc acgatttctc
    36721 tatagccagt ttgtatttgg cttaagaaaa aacttgggaa cagttaattg agaagccata
    36781 tcatgccatt gagataggcg gcaaaaagaa gccaaacgag ataggggagt aacaatatag
    36841 cgatgctttc tttcttattt tttaggctca aaatcagaag accagtgaga cccgccatca
    36901 caagaatcca taaaaaacca agcccagtcc aatgcagcac aaaaaaaagc ggggtccatg
    36961 cccaattcat cagcatctgt actgaatata aataaaacac agatctcatc tcagactcat
    37021 ttcgagagca ccataaaacc caagcaacta ttgcaagaag ggcatataaa atcgaccaaa
    37081 caattgaaaa tactggtcca ggaggagtta aaccggattt attgagctcc tcataccaag
    37141 agggaatatt cgcttgcgtc agcattccta aaaaaaaaac caattgcttc aaaggtaatg
    37201 atccaggcaa tcagctccgt ccaacttttt ttattcaatg gggtctctcc aaattttagt
    37261 taatctcttg tcaatccatt ccttttttta tttgctgata gtaactcaat gccttagttc
    37321 tcgcttcttg gtgatcaatg atgggtttgg gatatttttt ccctaaaaaa atggaattgg
    37381 cgtcagcatc tgtccagggc tgatgaattg actgactatt taattgggca agttcgggaa
    37441 tccattggcg tatgtaaatg ccatccgggt caaatttttg gctttgtaaa acaggattga
    37501 aaatacggaa ataaggtgct gcatccgcac cgcatcctgc tacccattgc cagctcgcgc
    37561 tgttattcgc cagatccgca tcgactaggg tgtctaaaaa ccactgtgcg cccgaacgcc
    37621 aatcaatcag taaatctttg gtaagaaatg atgcgacaat catgcgaacg cggttgtgca
    37681 tgtatcctgt ggcccataat tctctcattc cagcatcaat gataggatac ccagtcattc
    37741 ccttttgcca gcaagttaat aattgctcat cattttgcca ggggaatgca tcaaactcct
    37801 ttctaaaatt ttcgcaatcc agcttgggga agtggtagag cagatagaca gaaaactctc
    37861 tccaccccag ctctgataaa aaatgttcta cggaggccag atcacaggtt tgttccagtt
    37921 tagctaactc cagtgctctt aaaatcaccc aaggactgat ttccccaaag tgcaaatgag
    37981 gtgacaatcg agaggtcgca ttttttgctg gaaaatcgcg ctccctttta tatccgctaa
    38041 ggtgatgaac aataaactca tccagttttt tttgtgcacc ttcttctcca ggagtccaat
    38101 attcagaaaa tcgtgctgcc caattaattg ctggcaataa cttccatttg ccaaggtcat
    38161 cacttgtaat ttcgattccc cttgggcgtt tagtgagcat aaaaggcgag gggatattaa
    38221 gaatttgtcg acattgtttc cagtaaggag taaaaacctt aaaaaaatcc ccatttttat
    38281 ttttaattgt ccacggctca tgaagtaaat ttccattgaa actctgcact ttaatttctc
    38341 tttcgcccaa agcaagctta atttttttat cgcgtgcaat gacctgtggt tcatagcagc
    38401 gattccaata gactgattcc acaccatact gcgcgactaa ttccaaaata atctcttgcg
    38461 gatctccttt gcggagaacg aggcttaaac cctgttgatt taacgatttg cccagagcaa
    38521 tgagactatg atggagccac caggcttgag cttgccctaa aacactgcat ttatcatcat
    38581 aaatatagag tggaattaca atttcatgat gggagcatgc ttcaatgaac gcgggattat
    38641 cggtcaatct taaatcttga cggaaccaca caagagcaat agtcattctt ttcttctatt
    38701 gatggaaaaa ccataaaata acatgcttat gattttttct taactagagg agtccctttt
    38761 aatttctact aaacgcactt ttatttcaaa agatggctga taagggggag aagaagtata
    38821 ttgaatagac ctactaaaat catagccaaa ctggcaatgg cgccttctgt attccctatt
    38881 ttatgagcct gagcagttcc tgccgcatgg gctcctactc ctaaaagggc acctctggca
    38941 agagctgagt taaaaggcaa gcgcgacagg atgaactcac cgagtatagc tcccaatatc
    39001 cctgtgacaa taacaaaaat cgcagtcaaa tcaggtgccg caccaattcc ctttgagatt
    39061 tctatagcga acggtgtgct gatagagcga gggagcagac ttaaacgtaa attatcatca
    39121 aggccaagaa ggattgccaa tccccaactg gatactatgg cagtgaaact tcccacaatc
    39181 acgccaatta aaagtactgg ccaatgctgg cgaatgaggg cacgtttttc ataaatggga
    39241 atggcaaagg ctacggttgc aggaccgatt aacaagacta gccattttgt tccgcggaaa
    39301 tagtcctggt agtttacatg cagtaaaagc actaggctgg caactagtat tggtgttaaa
    39361 gcaagtggca ttagccacca aaagggccat cttctatata gtttttgggt aataaaatac
    39421 atgcctattg tgataagcga ccagaaaaat gcctggatca tggattcaga gagaatgctg
    39481 cttggcataa tgcgttctcc aacggtagca ataatctaca acaagggcag tgactagcat
    39541 caccgtgatt gtgctgagca gaataatgaa gagaatttta aggcctaaaa gaccgataaa
    39601 ttcctggtgc tccagtattg caaggacgcc agggataaaa aaaagtaaca tattggcaag
    39661 gagtaactca gccccccgtc ttatacgatt taattttaag caattcgtcg ctaaaagcag
    39721 taaaacaatt cccaagccaa ggattcctcc ggacaaagga agcttgacca gtttgagcaa
    39781 taactcacta gctaaccaaa ataagccaac caatactatc tgaaaaaatg aattttgctg
    39841 aattttgttt gccatatatg tgttcatggt ggatattatt ttttcattta agtttacact
    39901 tttaattaag atgaataaaa tgaatttata gactaataat ctttccaata tggaataatt
    39961 atggaattta aactgcttcg ttcttttata gaggttgtac ggcagggtag tttttccaaa
    40021 gcagctgaaa atctctttgc gacccagtct accgtgagta aagccataaa acagcttgaa
    40081 gaggagttag gtatcactct gattgagcgg tttaaaaagc gtaatgtacc aacaaccgca
    40141 ggtgaaattg tttatcagcg aggaacaaaa ttactggctg atcgagatga tcttcttaaa
    40201 gaattggatg aaatccgagg tttgaaacaa ggtcgactgc gtttgggtat ttctccggtg
    40261 ggcagcagta ctttatttgc ccctcttttt gttctttata gacaacatta tccaggcatt
    40321 gaagtagagc ttgttgaaca tggcagcgat aaacttgcag agtgtttgcg tgcaggagag
    40381 atagattttg ctggaacctt attacccatt tccgaagaat ttgattgcca attagtacgc
    40441 tctgaaccgt tggtcgcgct cctggcatct ggacatccat tagcaactaa acattgcatt
    40501 tctctaccag aacttaaaga aatacctttc atcctttttg gcagtggttt cgcactacat
    40561 cgaattatat tggacgcgtg ccgtcacgct ggtttcgaac ctaaggtggt ggctcaaagc
    40621 agtcagattg attttatgct ggagcttgtt gcagcaggcc ttggggttac ttttttgcca
    40681 cggatgattg ccactcaaag gcataagaca caaattcgct ctcttttact taatggaagt
    40741 gagttccaat ggaatatggc gatgacttgg cgtagaaatt cttatctctc cgaggctgca
    40801 aaagcttggt tggctctggt gcgcgaagtg catgttgata attagtttta gattggggaa
    40861 tgcaagagtt ctattttcct attgtccaaa tgaggcaggt gtattgaaaa tcattgctgc
    40921 tgtagccata cttcatatct aaactataat aaattagcaa aaccgtaatg aacaaccagc
    40981 aaaaaatcaa atgccccatt tgtggcaagc aaaatacctg gagccctgat aaccagttca
    41041 ggcctttttg ctccgaacga tgcaaattaa tagatctggg agaatgggcc agcgaaagcc
    41101 gaaaaattcc tggaagctcc attgatcccg agtctatagt gacaagcaat aataagcagg
    41161 ataatgagga tgagcagtag gaaacagaat ccaaagctca gctttccaaa ttgcgaataa
    41221 tattatgcac tgactgctat tgctcgcttt ctaactatcg gctacttatg aaatgtcaag
    41281 aagtaagttt ttattttttg cataacgttc caaatgagta tcgataaggg taacatgacc
    41341 taccttgcga tctggtcggg gtgcttttcc ataggcgtgg taatgaacgc ccgggatcga
    41401 taaacagggt tccaatggca gcagattgcc aatgcaattt aataaaaaac aagagccgct
    41461 tacctcagtg cttcccaagg gcaaatcaaa aagagcgcgc agatgatttt cgaattggct
    41521 agtatgtgct ccctcgatag tccagtggcc agaattatga acgcgtggtg ctatctcatt
    41581 cgcaatcaat tgattgcctt gataaaaaaa ttcaatggta agtacaccga cataattcag
    41641 tgcttctaaa attttgaatg catgagtttg ggcctcctgt tgtaaggcat cgtgatgaac
    41701 aggggcttct gagaaatgta aaatgccctt cctgtgatga tttttaatca atgggtagaa
    41761 acttgtttca ccactcttat tacgtacagc aattaacgaa aattcgcttt caaaggagat
    41821 taatttttca agaataagtg gttgaccgcc taacaggccc caactttttg aaatatccgc
    41881 ttgggttcgc aaaacatatt ggcccttgcc atcatagcca aaccttcttg tttttaacac
    41941 ggcaggtatt cctatgactg aaatagcctg tatcaattca tcttctgatt caaccggtaa
    42001 aaattctggt acaggaatat tgagagttct gaaaaaattt ttttcatgca atctgtcttg
    42061 cgtaattttc aaagcttggg ttgatggata gaaaggcttt atttgggcag ctaattcggc
    42121 gcaagaaaat ggtatatttt cggtttctat tgtgacgcaa tctacttctg acaaaaattg
    42181 ttttaaagcc aattcatcat taaaatcaga gactatcagc ctggtgagtt gaccagcaca
    42241 ggcgtcaggg ctgggatcca ggcagacagt gttaatccct aagggatatg ctgctaatgc
    42301 caacattctt gcaagttggc caccacctag aataccaagt ttcatgactt ttcctcttta
    42361 gggtttggat tgtcgagcac tttttgtgtt tgttgttctc gatagcgttt taaggcagct
    42421 cgaatatcag gatatttatt agcaagaatt gcagctgcaa acagtgcaga attaatggca
    42481 cctgctttac ctatcgataa agtccctaca ggtatccctg caggcatttg cacaatggat
    42541 agcagggaat cgacgccgtt taaagtttgt gacatgacag gaacacctaa aacaggcaaa
    42601 gatgttttgg ctgctaccat gcctgggaga tgagccgctc ctcctgcacc agcaataatc
    42661 acttccaaac ctcttttctc agcctgttcc gcatactgga aaagtttgtc gggtgttcta
    42721 tgagcggaga caatttctgc ctcatagcca atgttgagcg catccagtgt ttgggcggca
    42781 tggattaatg tttgccaatc actttgagaa cccataatta gacctattaa tatgggtttc
    42841 atagtgtgcc tctcttgttt gaatgtttat aacataccat ggatttgatt ctcgcaaacg
    42901 gttaaggatt acaataagga caataccttt ggtcagcaaa ttctagtcca tcgtctcgcg
    42961 cttgataagt cttaaattca cattttagac aatttaaaac ttttcgcagg taaagttctg
    43021 ttggtgttcc acaagaaatg caggctaaat gaccttcagc cgagattttt ccaaaccccg
    43081 gagtgtggca ttgagggcaa tattgctgaa tgcggtgaac cagtttcacc gcaagtttgt
    43141 taattacatt catccgcagc ggattcatca tcgctctcat atcggtttca aggcggatag
    43201 ttgtactgta tgtgaacgct ttttgaattg ctgttttaag ttgctcacgc tcacatattc
    43261 ctttttgaat ataattttct tcgctattta aggctcgaac aataatgccg tggcttggaa
    43321 attttgcttt gttgagaaac gcttcatagt catctgtggc actgatatca atatgactgt
    43381 aatttgtctc tgtagatact tcgtgctccg cgataattaa atcattatcc aagtcaataa
    43441 aggccatcaa ttcggtgtct gcgggacaaa aataaagctg gggatggggg ccgaaactcc
    43501 cttcgctgga tattacataa cggtatccaa attgcttggc tgctgcttta gctttcagaa
    43561 ttaatgtttc ataggctgag gcttttcttg gtatttcacc ggtgaatgtg ccgaactgat
    43621 cagtatcata atcagcaggc acatggatac gacagcctaa tttctcctca aaaggtggct
    43681 gaatcgcttt ttccttttga tgttttgagg ccagtaaaac acgctgattt tgatagtgca
    43741 taggggtcac ctccaaagtt aaatactaag agaaataatc gactctagca acagaaacat
    43801 ccgacacaaa caggactcct gaattctttt ggaatagagt tttcatgcca agcgcaatat
    43861 tagcaatagc ttcctcaggc gccactgcaa taaacatgac gaaagaggct ttgtcgttaa
    43921 acaaaatttt accttcatga aacccatgat gtccttttcc agagacgttt cgcaataagg
    43981 taaaaccaga aatatttgcc ttctccatca tagtagcgac catattttca ttgtcaccag
    44041 atacgatcac ttcgatcttt ttcatcggat gcaaagagat gttcatctct tttttttctg
    44101 atactttgat tatattcatc aggagactcc tttcactgaa ttacagacca gttattggtt
    44161 ttctctaact tgtaaaacaa gtgactccgc ggatcaatcg ctaccaaatg aacccattca
    44221 ttaaaaaaca aggtttttaa gacatcgtgt ttttcaacta gttcactgat gatttcgcga
    44281 ggagcgtaga ctatagttaa caggcgttgt ggctcatgaa aggatttgtc atcatggctc
    44341 atgacagatt gcagtggcaa tccatgcatt aaatcactgg cattgccttg catgactcca
    44401 atttttcctg cgacattatg agtgatttta ctgccacttc catatgccac gttgtcgatt
    44461 gttgaaaaca ggtattgagt attaatccat tgagcaacca ccataggtgc agtcaaaatg
    44521 gtttccagca aagcaccgtc tttgtcttgg gaccagtcat aagaatgtaa aaaacaacgt
    44581 ccttccagat taatgttttt ggtcagttgg cgaggtgcta cgatgaaagc ggcattgcga
    44641 gcaagccccc actctggcct tgtttcagac caatctgtgc ttcttcgtgc aatgtctttc
    44701 tccgcgcaat ctatggaatt caaatagtta attcgttcta aattgttatt gtgcttggcc
    44761 atgtttaagt cagttctaag ctggtccagg atatcttggt gaatgaattg actgacattt
    44821 gagtgataaa tttcaacctc atccgtcgtc gtgtcatgtt gcgcgccata aaacagggta
    44881 tcttgaggaa tattgattcc attttctgcc agagcccttc tcactattat tttattgagg
    44941 atagacgcca gtaattgcgc attttttcca ccttgattac ctccacaagc gccgcaatcc
    45001 agggcggaag cataagggtt attttgagta gagctgccat gaccacagaa aataacaagt
    45061 ttggcaaagt tatcagtcag tcccataagc cttaatgcca tttcagcata ggcaatttgt
    45121 tcttgaagtg agatacctac ctctttctct aataaatcca attcaaaaac aggttgtgtt
    45181 tgaatggagg ggcatatcat ttcatttagg tctttggtaa ggcgtctagc gaaaattggg
    45241 ctacatgatt taaggaacat agtaatccca caccaaattc ctaatgattc taccagagca
    45301 aatggggttg aaaaattata ctttaattgc tgatagaccc tgttcaaaat atttttaaat
    45361 tctttacctt tctcatgatg ctctaagcag tgttcatttg cagctattgc tttttcatgg
    45421 atattaaatc gtggttttaa aaggactggg caggagtctt ttattgtttc accgtcataa
    45481 tccttgatgc tcacaggtaa accaaagaat cctgcaaatc ccaaggtctc gtagtgtccg
    45541 agtttttcta tacatcgccg aaacggttct gagcgtacat caatacaaaa aaccatttgg
    45601 gcattagctc tattttcttt tatatgagct ttgcttaact caggcaaaag cttttttaaa
    45661 agcaattgcc tgtaatcgtc ttctttattt tttatatttt gaataagttg tttagtatcc
    45721 gcactatctt ttttcttttt tttttcttcc tggcttgctt ccggccataa tatgcaggtg
    45781 attaccagcc tcactgcaat aaaatcgaca aggctaacag gattttcttc cttgccattg
    45841 cgatttttcc attcgcttat ccacttaatg tatccagccc agccaggtaa gtgagacaga
    45901 gtggattgga gaaaatcctc ttgcttttgg tttaatacgc caagtttgtc gaggcaaagt
    45961 cggatagctt gctcaggcat ttctggcagg tttgataacc agctctttat tgatttactg
    46021 ttttgatgca gtgcgctgtc aaaaggggct agttttaaaa aaccaaaata taagcctttg
    46081 tcacgatgag gcatttcaag cgtgccttgt ccaatgtcca gaaagctgcc acaccatttg
    46141 atcatgtgcc aattgactct ttccagtttt tcattgaatg gagtgccttt ttttgccgtt
    46201 tggttttgta tcacagcctc ttcaaaggac atggattcaa aaccatgcaa ggcattgcag
    46261 gcaataaatt tttccaaagg ccatacaggt gtcatttgtt ttgcaacatt atttaccatt
    46321 gcccggataa tcatgacatc attatcaact ttgtagttaa attgggtcat tttattttca
    46381 ttgtttgtgt tagttttgat tagtgtgatg tggctactca tgttggctcc ttttcttaat
    46441 agtaataagt atggcgattt gccgtaatgg ttttggggtg aggcgtactt gcgttaaaca
    46501 tgtacatgta aaaccaatcc caaagtctgg tctctctgat ttttttgaat aaccccatgc
    46561 tgaatacaag ccaaagggaa aagaagataa tcagagttaa gacttgtaag ctgcttaatt
    46621 ggtattgagg aaatcctgtt agattaggta agagtgactc gatgagatgg atacttcccc
    46681 cataaaaaat tccaattgag aaaactaaag ccaagactaa aattactttt ttgaaatgct
    46741 tctcatatgc aatcaggccc agggtgagtt gtgcccctgt gatgaatgca aaacctaaaa
    46801 gaaaggtgga cgcctggaga gataagatgg atttccctgt gataagggca aaaccataca
    46861 ttccaccaag tcctcctaaa aatgcagtca ataagtttgt caatttttcg ggacgtgatt
    46921 tattttgata tttcttttgt tggacggcag agcccgaatt gagaaaaaga taggatttaa
    46981 acaagccatg ccagcataga tgggcgatag cggccgggaa aagtcctaag ccacattgca
    47041 tcatcataaa acccatttgt gccatggtgg aactggcaag catacttttg atattccact
    47101 gcaataattt ccagagggtg cctaataatg cagtcagtgc gccgaaaatg aataaaacgg
    47161 tcaataaatt tgctttcgct acgaacagcg gagcaaattt aacaatcaga aagcccccac
    47221 cattaactaa tccagcatgc attagagcag ataccggtgt tggagagttt aatgagctca
    47281 ttagccagcg atgaaaaggc catatggctg attgtgtcat cgctgttaag ataataaatc
    47341 caagtgccac agcaatggca ggggatgact tattgctcac ttcgctggtt attgtttcaa
    47401 ttgacagagt attgtttaat gtaaaaagaa tccaaaatgc agtgataagc aacaagcttc
    47461 ctggtaataa tgtttttaaa gtcagtatcc ctgagttgta ggctgctttc cattgtgact
    47521 tatgaatcat gaggtttatc aagagcaaat tactagctga ccaggcaagc cagaacaata
    47581 aaagattgtc tgctaaagcc atcacaacag ttgttaaagt tatggctgaa agtttgaaaa
    47641 aataacgtcg gtagcacttg tccccatgca tgtatcgtag ggaaaaacgg tggataataa
    47701 agctgatgaa taatattaat gtgcttagta agaggcttag tgtgctggca tgaaataaaa
    47761 aataactgtt tgggtttaca gtatttgcgg aaacaattaa tgccaagaga ctcaatccaa
    47821 aaccaaaacc tataactgtg gtaccgtaat aagttgcttg aatatttctt gtttttccta
    47881 acaagctata gaatagcatc gctatcaagg gtgatagcat gagtgaaatc gataacccat
    47941 ttaaaagttt atccacatta attctccatt agaaaatctg ttattattgt ggaaatatat
    48001 tagatatgtt aatataagta aaattaaaaa taataattta aactattaga aaaacttatg
    48061 tcaattgata caatcacact acaatgcttt ctggcagtgg ctgaaacgca aagttttacc
    48121 aaggcggcac agcgggttgg acgcacccaa tcggctatca gtcagcaaat agctaaattg
    48181 gaaaacctga ttgaaaaacc tctaattaat cgtggccgag aactttctct gaccacggat
    48241 ggtgaaatat ttttagggta tgccaaacga atttatgaat tgcatcgaga atctttagat
    48301 cgttttaaag cgcctgagtt acaaggtgaa atacgcttcg gattgccaga ggattttgcc
    48361 agcattattt tatcagatgt tctcgtggag ttttcaagat tacatcctcg tgtgctattg
    48421 aatgtggaat gtgacttgac gcttaatctc attgagcgtt ttaatgaagg taaatttgat
    48481 ttaattctta ttaaaacaac tcagcaacag attggtactg agtgtattga agtgtgcata
    48541 gaacctgttg agtggatagg caaaaaagaa ttgctacctc ttttagacaa accagggccg
    48601 atccctttag tgctttcacc aacaccttgt gtttatcggg gaaatgtcat tgatgctttg
    48661 gataaagctc acatcaactg gcgccttgta tacagtagtg caagttatgc gggaaaaatg
    48721 gctgctgtaa gggcgggatt agggattact gctattcagc gcagcatgat cccaaactat
    48781 ctggaacggc tcgattacga ttttctccct atattgaatg atgtccatgt ttctttatta
    48841 aaaaatagtg aaagcaataa agcgattgaa tcgctggaat tttttatctt gcaaaaatta
    48901 aaatattaat agcattaaca agaatcaagg gtaaaaggga tgtttaggga atgaatgcaa
    48961 aaccaattaa ttcaattatt gatgtattag atcgccaaac aatctcatcc atcgcgatga
    49021 ttagtgtaat tatcagctta ctgtcattga gtatccccat tgctgcccaa acactcgtga
    49081 atttaattgc ttttggcaag ctattgcaac ccgtgattac gttaagtttg atggtattga
    49141 ttttaatgat ggctctaggc gctctccaca tatggcaaat tgtcattatt gaagtaattc
    49201 agcaaaaact gatggttaaa gtcagtttaa gtctgacacg acatttcacc cacatgtctc
    49261 ttgataattt ttcgacacat catggaccgg agttggtcaa tcgctttttt gaaatagtca
    49321 ctatcaaaaa atcactcgcg agtttattgt tatatggcat taatttgggg ttacaacttt
    49381 tttttggact attgttgatt ctaatctatc atcctctttt ccttttgttt gatattttca
    49441 tcattcttgg cattctactg atcattttta tcccttacaa aaaggcatta gacagcgcca
    49501 aaaaagaatg ttctgaaaaa catgttgtag gtgcttggtt agaagaaatt ttgattaacc
    49561 ggtatctgtt ccgttttagt ttatatcatc gctatgtgac tcaacaaacg gataaaaaat
    49621 tggttgcttt cctaaaagca agaaataccc attttaagca gcttatcaaa catcaaattg
    49681 gtttttacac gctgtctgct ttggccagca gtcttttatt gggattgggg ggctatcttg
    49741 ttattaataa tcagttaagt cttggacaat tggttgccgc agaaatcgtt ttaggtgctc
    49801 tgatttactc gtttaaacgc tttggtgccc ttttagaaaa ttattatgat ctggtcgcct
    49861 ctgaaaataa aattgatgcg gtacttaatc tgtcacttga acaggtgaaa gaagatgagc
    49921 atggtgagtt attgactcca attaatagca tccagctaga gatagatagc aagagcaagg
    49981 cattgataac agttgataag cctttattgg tgttttcgga aaaatcggat ttatgccaat
    50041 tgtttgttga gcagctattt ggattccaag atcctgcctc acttcatgtt cttattaatg
    50101 agattccttg cgcgagaaag aaccttattt ctcttcgtcg ttttagttta ttgattgcag
    50161 agccccagtg gtttaccgga agcatttatg acaacttagt ccttaatcat cgcaacgtgc
    50221 ctaacaaaat cattattgaa caattaaaga atgttggact tgctgataaa atcatacaac
    50281 tacctgaagg tctgcgcact attctctatg aatggcagga tgtatttacc tcagttgagc
    50341 tcaccaaatt gatgttagtc agggctattc tattacaacc agaattaatc gttatcgatt
    50401 gcgcttttga catccttaat catcaagaag tgaatgaaat gatggaattg gtattgtctt
    50461 taaaaaatac gctgctagtg atcgtaagcc aaagatccga ttttaactat ttaaataatc
    50521 gcctggtgat ttcctcatga aattacatgc ttattctatg attagtaaac tgcccaagcc
    50581 tcaaaaaata gcgaaaataa ttttcattgt ttttgttgcc attattcttt tcttaagttt
    50641 tactccgtgg caacagtttg cattaggcaa cggaaaagtc atcgcttttt caccaacaga
    50701 gaggcaacat acagttaatt cacctattag cggacgaata aagaaatggt atgtcgacga
    50761 aggcatgcgt gtgaaaccag gtgatccaat tgtcgatatt actgataatg atccggaact
    50821 tttgtcacgt cttgaagttg agaaaaaggc gattttgctt cgtatagaag cagccgaaca
    50881 agctattgct gcaggtaaag ccaatgttga cagacaaaag aaattgtatg aacaggggat
    50941 taattcgaga aggcaatatg aattggcaca aattgaatat gcaaaatacc aaaatgaact
    51001 tgcgcaggcg aatattgata aagtgaatgt cgatgttcga attgctcgtc aaaaaacaca
    51061 aatcattaaa gcccatgttg caggtatcat ctttagacgt ttgacaggtc aggaaagtgt
    51121 cgttgtgaac gcgggcgatg tattagctca aattctgcct gaaacagagt ctcgcgccgt
    51181 tgaattatgg gttgatggca atgatatacc tttcgtacgc cttaatcaaa aagcccggtt
    51241 gcaatttgaa ggatggcctg ctattcaatt tcgcggatgg ccagaaattg cagtcggaac
    51301 atttggaggt acagtctcct ttattgatcc tacagataat gggtttggtt tattccgtgt
    51361 agtgattgtt cctaatgagc catggcctag tgtgcgtttt ttacggcaag gagtgcgggt
    51421 gcatgggtgg gttcaattgg gtcaagttcc attatggtat gagctttggc gtcagtataa
    51481 tggtttcccg ccggaaagtg atcgcgagaa taaagcatca tgatttgttt tccttcaggc
    51541 aaaaacagat tgattgtctt atgtttgctc ttgatattgt cattcaagtg ttttccaata
    51601 ccaggcaaac ctttgctgct tgatgccgta cttaaaagtg tggatcgaag ttaccctcag
    51661 atagtcatag cgcgtcttga aatcagaaaa gcggaaggtg attatgtcaa tgcattagga
    51721 aaatttgacc cctctttcga tgttaatccc cgcttccagc ccgttggggg gtatattaat
    51781 aattatgcag ataatctttt taatgtgccc accctctata atggacttaa attgtttgga
    51841 ggctatcgta ttggtagagg tgattggcca atttactatc aaaattacct gactaactct
    51901 ggtggtgagt atcgtgcagg cctttcctta cctttgttga gagacagacg aattgataaa
    51961 gagcgtaccg agctttttac aagagaagaa acaattcgca tgaaacaaca ggatgctgct
    52021 gccacaaaaa ttaaaattta tcaggaaacc attaaagcct attggcaatg ggttgaggcg
    52081 ggtttccagt taaaaacttt caggcatttg ctaaatttag cgaaagagcg gcaaaatgcg
    52141 attattcaac aagctcaaca aggtgatttg cctagacttg ccattgcaga aaatttacaa
    52201 cttattgttc aacgtgaaca actggtcaat caaggagaaa tgttattggc gcaagctggt
    52261 atcaatcttt cactttacta tcgtgacgcg aatggcaaac cgcaaaaacc ggatgaaagt
    52321 caattaccgg caacatttcc tgcccaacca attcgagtat ccaatagtat ttcacagtta
    52381 caacggcatc ctgttttgcg taaattggag aattattcaa aaattgttaa attaaaacaa
    52441 aatttagcaa gaaatgagct tcttcctaat cttgatgcga cagcttatac ttttaaacaa
    52501 tatggaacag gcggttatcc tttattgatc cctcaggctg ctatgatcgg cgtgaatttt
    52561 aaatttccta tttttcaacg tgaagcaaga ggaaagctta tcagcaccac gagcgaacta
    52621 cagcaagtaa aaacggaaat gaagtttacc tacgaacagt taaataacga gctgtccaat
    52681 ctcttggttg caataagaat atatcagcag caagtggttt tgcttaataa agagttaaat
    52741 cttgcacggc aagtagagaa gggggaaacc aagagatttt atgaaggtga tagcacgctt
    52801 tttttagtga atcaacgtga acaaacaaca gcacaagtca agttaaattg gattaacgca
    52861 gaggtcaatt tacaagaggc tacggcttta gcgagatttt tttcttccac gcaataataa
    52921 cggatatcaa ttggagaccc aagtgataaa tatcttgctt acccacaggt gaggcaaaag
    52981 ataattgtta gctacttgca ctcccccttg gcatatgtta cataatgttt acttaatgtt
    53041 catattttat aatgcttgac tgaaacatta aatcattaat cctaaaaggg agtgtacaga
    53101 tgtcatattc aatagataat cctggtggcg gggattgtgg attttatgct cttgctattg
    53161 gtttaataag catcatacaa aatgaataca aacttcacgg aaaaagtaaa acttatgatc
    53221 gatggaagca agtcgaaggt atttatggcg tacgacagca agacatcctt aatgtcgatc
    53281 taacaaaact tcatgattcc ccctacactt ataaaagtga attattattc ctattgcaga
    53341 tgtctttacg aaatattgca gtcattgtga ataaagaaga tttgatcaag agaataatcg
    53401 aagaaggtcg aagtaaagat caaaccaaaa tagaaggatc aaatgcttat tgcaaattca
    53461 tggaattggt acaactttat ctctatcgaa gagactcgct ggcaaggata agtcaattca
    53521 atgaactagc cctctctccg gaaattgttc aaacagcgcg agatattgcc aaaatactga
    53581 gaccaatcct cagaaaccag ccttttcatc aggtacagaa aatagaaaat gcttttgtta
    53641 aagaagtctt actaaacgat gttctatcag gcaatacaat caattcaaat tcaattattt
    53701 tgaagaacct tgaaaaaatt aaagaacaag gacgatgggc aacgcatgat gatttgaaag
    53761 aaattgccga cctacttaaa atcaacctca acgttgttgg acaaccgaat ggcaaaccta
    53821 ttctggaata tcctacggtc actctaaaga acctgagtaa tacacattgg atcacacaag
    53881 tagaatcact tcagggaccc agacaataca gcacaccccg taagcgagct tttggttatg
    53941 aaactgtatc tgacagagcc gttcctttaa aagaagagca aactcttgac agggccttca
    54001 gaaccaaaac tcaatccgag agaacaaaac gttaccacca acattttgaa acgctgtttg
    54061 atactttatc gccttatggt ttctttactt atgagcagaa taaaaggaat actcaggttt
    54121 ctggcaaagt gcaaaccaga ttggagtccg atgagtcttt ggccgctcaa ttacaggaag
    54181 cggaacttag acgaatctta tactgatgcg atcgctttat cgtaaaaaat gatgggaact
    54241 caatatgcat cagggtacct tggcaggaag caggcaagtt acccttgatg cattttaccc
    54301 ataagaagtc atgacagtga actacaaaac agacaaggat aatcaggaag cgtttttatt
    54361 ttgtcgcttt gctacctcaa gaattgtata ctggcaattc tgtaacatta ccaaccatgt
    54421 cattgtcatg acggggtgtc gctaaaactt gagacatgta aagtattaaa atgctataag
    54481 gcaagaagca gattaaatcg aaagggaatt tcagtaaacc aatgccgcca aaagatccta
    54541 aataagaaat aatagaaaat gatcccatgt acatgataaa ccacaccaga ggcgagccat
    54601 ttctcataga aatatggcga tggtaaaaaa agtaaatacc aagccccata agcaatgcta
    54661 agtttagctt ccaaataata gaaaaaccac accagtacaa catgagatta catatataaa
    54721 aggcgatatg tgacaagaaa atactatttg cgagcttgaa aggtctatcc atatgggggt
    54781 gcattttacg catggccaag agacaaatcg ggccaattcc gtaggataaa atacttgctg
    54841 atgaaaggaa tgccactagt ttttgccatc ccggaaaggg taagaatgca aaagaaccaa
    54901 ccaaaagatt tgcataaagc gttacgtaag gtatgttatg gcggttgacc tttaagaaaa
    54961 acttgggcaa atgttggttt aatgccatgc catacaaaat acgagatgta gctgcggtat
    55021 aaactaaagt cgtgccaaaa ggagaaaaag cggcgtcaaa caataacaga gtagcaacaa
    55081 tgccaagtcc taacaaaaga gttaatccaa ccaatgggcc ttcatcaccg gggtaactca
    55141 atgcgtgcca accttgagcc aaatactttt gtggtaccgc cgctataaaa ctgaactgca
    55201 acataaaata aagaataaag ccaaccaata ccgcgccaag tatggcaata ggaatatttc
    55261 gttgtggatt tttaacttct cctgccagga ttaaaccatt ttgaaaccct gtaaaagcaa
    55321 aagctacacc gcctgcagaa agagcggtaa atatctctac ccagtgttct ttgcttgaca
    55381 gattaatatt cacattagca agagagggtg attgctttaa cagtgaaata atagcgatac
    55441 tcggcaaaac aaatttaatg atgctggcat acttgttaca ctcggctaaa aacttaatcc
    55501 cataagaatt caaaataact acgaaaaaca taatcccaat tgcagcaaga taaccataat
    55561 tggacagggt gagtgattcg ctatgattgt cgattaatat tggaaaaaaa tggcttgaat
    55621 attgcaaaat tgcttgtatc tcaataggtg tcataacaac ataagataac caggatgtcc
    55681 atgcgaataa aaaacccact tctcgaccat gtgtgtaagt agggtaatta gacatacccc
    55741 cggaaactgg aaacatcgtt cccagttcac aaagcggtag ggcaataaac aacataaata
    55801 ttgctgcaat aacccagctt atcagagaat tactgcctgc catttgggca ctaataaatg
    55861 ggctgaacag ccaacctgaa ccaatcattc caccagctga tgcaactaat atattcaggg
    55921 ttgaaatatc ccgttttaac atgggttact gttccatttg tttgtcaaaa caacactatc
    55981 tgttcgatat tgttcaagtt tctataaaag tgggtaatga ttatatataa agaaataaat
    56041 cattgtaaat cttaatatcc aggccacaat aacattctga cgatgggcct taacgagggg
    56101 cgtttgctat aggatttgtc attggattta agaaatccat caatagtttt agaattaatc
    56161 caagtgcaac cagactgacg ctgatagctg caagtgagta tgtgggcagc ttcaggtgat
    56221 gagtgtgcat atcacataaa tcatcgtaga tattgctcat ggtttcatag atgcctttgc
    56281 attgctcaag aagctcttga tactcatcac cagttagaac aatgttatcg atttgtttca
    56341 tcatatcctg ataaagagcg agtggtgaat gttttcccga ggataaaaag gagatggcag
    56401 cagaaaaatc gtattgatct gccgttattt ggtaggtggt caagcgattg cttggttcaa
    56461 tgtatttcga gcggataata ttcccgccat gcataaaccc ggcaatatgt aataaaaaat
    56521 gagcaagaag gattttgggt gtaaattttt ctatgttttc aaggtaattt tcagttgttt
    56581 ttgttatcac atccttatga atttcatcag gattaatgtt tagttgtcgt aaatcatttt
    56641 ctatagcagg agtacgccat aggtgctcaa gataagataa agcaaaaaat gcgctgattt
    56701 ctgactgttc tgtttttggc aaattttgta attttgtttc tatggcttta ataatcaaaa
    56761 aatgctggat caagcgtgag gcgtataagt ctttagggat ctctgtattt ttaaacagat
    56821 attctgtttt aaagcggtga tgctctgctt tttcatgttc ttctgtaagt tggccgggtt
    56881 ttccattttt ataggttgca ctgattaggg ctttgctaaa cattcgagat aattccttga
    56941 tagagcatga atagaagaga ttatgcctga tggctgcgca ttatatatta tcgtatacgg
    57001 ggttgctact aaaaataccg tattcgtccg accaagtccg gtttgaacgg atacaaataa
    57061 ttacgacagt aagaaattta tagaattttt ttggcagatt acgccgtcag gtaaaacaga
    57121 aagttttttt gattgtggat aatcatctca tgcatcacag cagaaaagta atgaaatatg
    57181 ttgagaaatt taaagatgag atagaaattt tttttgcctg tatattcccg agaattaaat
    57241 ccgcaagagt tggtcaatca ggatgtgaaa gctaatgcct gcttatttaa accagtgaga
    57301 tgtgtgaatg acttattcat aaacatcaga ctttacctga ctaaagcaca atttaatgaa
    57361 tttaagatta tggatttttt taagaaaaat gaaactaaat atgccgcatg ggaa
//
LOCUS       DAXLDT010000003        52861 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXLDT010000003 DAXLDT010000000
VERSION     DAXLDT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 52861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52861
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.003"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            213..989
                     /locus_tag="ACF2SP_000123"
     CDS             213..989
                     /locus_tag="ACF2SP_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357939.1"
                     /translation="MRGKREFLKHEYSPGHWSIDYTRAGTSIAVITVRNKYHYSVILN
                     PTDCRGYRIIIRYLNEGDSSLSSAFNRPYTVSEQRGLNDVASLMTQVYEKLGLIVQFS
                     QLGNNSQSFDKETGVTLIGSEEEPSMLHLHMWGRGDPDMEYIAGVPLRGPEPGLMFDL
                     IAKNKTHPINQHAIKWNDEELKACLAMFKLKLAEYVNSPEFTEEFGDTLKVTIHDKKD
                     LGEEKESGFGFFHKMKSASDGITTLEKTLALEDNKINPFV"
     gene            complement(1811..3811)
                     /gene="ceg14"
                     /locus_tag="ACF2SP_000124"
     CDS             complement(1811..3811)
                     /gene="ceg14"
                     /locus_tag="ACF2SP_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg14/sidL"
                     /protein_id="HFD2357940.1"
                     /translation="MQNLDEILKKLGEDLKKTSKETANKLSVLSRKVAEDVVRVSDET
                     VQKINEVTKKFSVDTTVSTIDTQKILDDFMKNIRFSRFKQIWQAIEETPKSKRILLKS
                     LFLKGLLTHNKPLLEEIIRQIELIPYSSDLEMLGAFSQDKAHPLSPELLEFVQEMLAN
                     ESEKVLLTILMNAFQSIPELSLDSKTGTLSMKTKKELLPLLIAKVDTSIAKSIMSQLT
                     DISFDSVLPAFRPSIGDPSYQETKINLSKYLSLVGDKTDISEFLILTIGLFSSLKIAD
                     KGFLQELSADYLFVRYDDCLHKIIEKLKEKEVVEQLGEKQVQKIMESSGNLKRFKPTR
                     NDPRRFFETRLAQYINGLSHSEKTLEIDLTEESLEPEDEELRDNTLKACNKILQFFLG
                     DCGRVDTPREMEQKICIFANGYKDTSKFTKKEVEFITGVLHHAEAHKGRDRVERDKLT
                     GIKERKFETDSEKVGSDVWNCGGGVMKGCSPVFYDAQREPMRNMLVDSSTVAPDNKEG
                     KQWFWNNNRQYSSYVGSISGHTCNIVGMLAKYMENYKEDPDLQNDINLFLIQVIGVYA
                     KRGFHAMLEVIDVLHDPYVQDIFKGYGVQVNLYSYFKENPELAGFLQHAMNDATTYTQ
                     ALVNKKHVKEALKSNSFFSKKDEESGDKKNDHTPKDGTVYGC"
     gene            4073..4882
                     /gene="ankJ"
                     /locus_tag="ACF2SP_000125"
     CDS             4073..4882
                     /gene="ankJ"
                     /locus_tag="ACF2SP_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkJ/LegA11"
                     /protein_id="HFD2357941.1"
                     /translation="MITMGRSEMKIASAELRELMKAVSEGHYETVNTILDKNPELVNQ
                     YVPPTYDSPLARVLNKKHIDYKMLDILVKHHVDFDYPINHHKETPIELACKNQDLQLF
                     KYLVQHNAPISEHAPHFLLVNSTNIKYLTEDKIKNTCEIIKLMGGLEAVSSKCDAEGN
                     RFEELARKSQLINRFGGIVRYDYVQLLRSIYPIVDREVDVPTINASTEKLTDLLNKIR
                     DQHTSKEGYDQQNLKDSISLFFMTGGEVPPSRKVPESRFEEAGIDAPKNAL"
     gene            complement(5036..5647)
                     /locus_tag="ACF2SP_000126"
     CDS             complement(5036..5647)
                     /locus_tag="ACF2SP_000126"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2357942.1"
                     /translation="MNKRNWTAYYNSTKQNTLPRKSLLKAIANFDKEKINLSKSAIDL
                     GCGAGIDVMELLRCGWSVIAIDSQLSAIDSLLSSAQELTCTNKLKTIVSSFEALDSLP
                     KVNLINASFSLPFLKPVYFYKFWNVILKGLKPGGRFSGSFFGFNDFWNTRTDMIFLNR
                     EMICHLFSQFKIEYFQEEEKDEMDALGYYKHWHKFFIVAKKIA"
     gene            5909..6340
                     /locus_tag="ACF2SP_000127"
     CDS             5909..6340
                     /locus_tag="ACF2SP_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357943.1"
                     /translation="MHPDLKDLLDYRNHKIITRYNMDYPTAAMQAEEALHELMKFIWL
                     CHKHKTDKLLYPDDKNLDFSCVIHAEMADIDKMWHTFLLFTRDYHEFCDTCLNGVFFH
                     HDPLIGENTPVSEEDYALELTRYLTYIHDHLGSETLIKWFQ"
     gene            complement(6342..6722)
                     /locus_tag="ACF2SP_000128"
     CDS             complement(6342..6722)
                     /locus_tag="ACF2SP_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFD2357944.1"
                     /translation="MSHPDYSVYNEKCPSHNVLEGISDKWSILVISLLSQKIYRFGEL
                     KREIGGISPKMLTQTLLKLERYGFVQRQSYPVLPMKVEYSLTRLGKELSAILVLLTRW
                     TETNMDKIINAEKTFCEQKLGCHA"
     gene            complement(6744..7631)
                     /locus_tag="ACF2SP_000129"
     CDS             complement(6744..7631)
                     /locus_tag="ACF2SP_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD2357945.1"
                     /translation="MHDTSALKKKLDYARFIHLVIMFMVAMLIFLFSTFPEKWWVLIT
                     VLSVSAGIEPGLIIRRAKHRIGGTLLALIILIPLLYLLQLNYRLISILFVLAIVGLSV
                     ATLNTRRYDISVFFITLVVFFLMAQTEEATSPRGPFEMVLNRGICTLLGVFIVLAGDY
                     FLFQAYRYSHRLYFFHQVMVYDFLNDIVRKIDESNTEKINTLLFVEKLRGQFTEHYLP
                     ISISSENLKLDFKTSEHTKKRIDIFQETIWEIRRLVFALCISEFVLHSSTASEQHLQR
                     FKLLMNKARTHFIQFEGRY"
     gene            complement(7610..8542)
                     /locus_tag="ACF2SP_000130"
     CDS             complement(7610..8542)
                     /locus_tag="ACF2SP_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357946.1"
                     /translation="MSMSKFIEWIDEVDPYAVQRIALYKSLFIATVMAYVYWVFRPTN
                     FTAFFSPFLLVRFYESPSLTSFKEKEHFLIFIGVVVVLLSTSFYLVYPFRVVFFFFSI
                     IALASVYFYVLKNYLPLRNVTMLIIASGATILSTEPPANWQIVYDIVGSSALSMIAIF
                     ICLRFFPNQYLSVWKRALAKFIQSLELDIEGSFTHRGPKFMQEEMTHLGMLRQYRRLI
                     PKKYMIYTQRISINIRNIQFSLDNLYYEAKNEAFWHGVKENLYRLRCAIKTNTFCGTP
                     KMSIMPESKLQQYVMRCLQQAFSQWNQLCMILQH"
     gene            complement(8520..9557)
                     /locus_tag="ACF2SP_000131"
     CDS             complement(8520..9557)
                     /locus_tag="ACF2SP_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFD2357947.1"
                     /translation="MKKLMEYGGKIKEYLLRLKPKRWFTLTNVIIFIGFLTAIIYVFS
                     YLIPFTDNAFVVNNVRPVAALTSGYITDLYVKNGDAVRKGQKLFTVFKKPYEYTVEQL
                     SADLAGAQAKLAVLKATYERDLKLSENEQKIYKKLAQDDQKYLKGYAIKSVSLITLQN
                     SQQETKAAKDKWQASLKQLEIDQHQITVQENEIKSIQARLKNAKVNLDLTDVYAQGNG
                     VIQNLFFTIGTPVNINQPLFSLVDQDNIYIQANFNETDLRDVRKGSKVLIFPRMYLGR
                     KIFHGVIDSDYWSANRQLVDDRTQLQNVINENQWILLPQRLPVIIKVTDPDPKYPLRV
                     GASAYVYVEVY"
     gene            complement(9697..11049)
                     /locus_tag="ACF2SP_000132"
     CDS             complement(9697..11049)
                     /locus_tag="ACF2SP_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2357948.1"
                     /translation="MMCMLKKLMFLLLLGAGLLSCGQSTDQQISNITTPKQFPSSPRD
                     YRPVQELPYLAWWKQFQDPELDRLIDTGLKTNMDIHIAMANLQQAQGELQQVKLSWIP
                     NVQILGGYSTNPALGVPGGFYGIWPSYILNIMQLYTQQKRAQYQVQYHQAAIDGLRLT
                     LIGQVAAAYFTLIAQLEQLKLLYELDRDLKSLIALSRKEINIGLKNDIDLAQLQSNER
                     LIAAQIKPIKHNIVVSQNALRYLINENPGLIKSKNNFAQLDFTRFKPGSLPATVLGNR
                     PDMKMAEYSLKAARAGIFVAYSDFFPVMQLDDFIGEAHLPDSTFEQATDAYVNWTINP
                     NTLGKISVSKGVYNAKLAEYVKTVRLILKEVDNAFSANKRMREQFLSYRHAQEDYQHK
                     YKLQNGLLKSGLMSYKELLESKVYLDTLALSTNQAKLELAMSLVMLYQDLAGGYACSD
                     "
     gene            11136..11495
                     /locus_tag="ACF2SP_000133"
     CDS             11136..11495
                     /locus_tag="ACF2SP_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2357949.1"
                     /translation="MIKKIAFTMYPVQNMDRARQFYEKTIGLKVGKISAEGAWVEYDL
                     PQGGCFAITTLAQGVIPSANSGGSVAFEVDNLDDLVFQLKSKGVQFKIDLFASPVCRM
                     AIAVDSEGNAFTLHQLT"
     gene            complement(11598..12011)
                     /locus_tag="ACF2SP_000134"
     CDS             complement(11598..12011)
                     /locus_tag="ACF2SP_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HFD2357950.1"
                     /translation="MFCIDDRIKSSSFILGEWPLSTVLLKNDASYPWFILVPRKMDIQ
                     EIYQLEKSERFLLIEEINQLSLLVRDYFKPDKLNIGALGNIVPQLHIHIVIRSKNDPL
                     WPQGIWQSSIPSIPYPESELKSLLPTLGDLVNKAY"
     gene            12248..12481
                     /locus_tag="ACF2SP_000135"
     CDS             12248..12481
                     /locus_tag="ACF2SP_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD2357951.1"
                     /translation="MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLVP
                     GQAVTYDVVNGERGLHASNVVALSENAEQEEIA"
     gene            12592..13587
                     /gene="hemH"
                     /locus_tag="ACF2SP_000136"
     CDS             12592..13587
                     /gene="hemH"
                     /locus_tag="ACF2SP_000136"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFD2357952.1"
                     /translation="MRRGLLLLNLGTPDNADIRAVKLYLREFLTDKRVIDLPTIPRYI
                     LVYCLILPFRSPKSAQAYQSIWTEKGSPLLYHSQNLVTKLQSSLKDEYKIALGMRYGT
                     PSITTALAELKDCHSLTILPLFPQYSSAATGSAIEKTLSYLANQEIIPSIKIIRDFYQ
                     RPEYIQAQAKIMKPYIKDNFHVLFSYHGIPERHIHKSGCDTLCPQTCTPIYDKNQACY
                     RAQCYQTSLLLAKELQLGTHQYTTAFQSRLGKTPWIKPYTDEIFAELISKGIKNIVVS
                     CPSFVADCLETLEEIGIRAKEQWEKLGGEQFILTPCMNDHPEWIKAIHSIVNEQI"
     gene            13715..14254
                     /locus_tag="ACF2SP_000137"
     CDS             13715..14254
                     /locus_tag="ACF2SP_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFD2357953.1"
                     /translation="MKKIKSVSHLLFLLFKTLAYGLPLVTTYFILFHFESMLNWGGWH
                     SLLSTPVKDPSHFSLLHRFIILLIEALPLSINVLICHQLAKLFRLYEHGYLFEEENIR
                     LIKNISIYLISGELIQLLYQPLITAALTFTNPSGQRIASITLGTANLSTLITAFIILI
                     ASWIIKEAHKLKTDALLTI"
     gene            14341..14577
                     /locus_tag="ACF2SP_000138"
     CDS             14341..14577
                     /locus_tag="ACF2SP_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2357954.1"
                     /translation="MSIIVNLDVMMAKRKCRLRDLAEAIGITEANLSILKNGKAKAIR
                     FATLEAICAYLHCQPGDLLEYQSTEDNHLIKDRA"
     gene            14703..16052
                     /gene="legY"
                     /locus_tag="ACF2SP_000139"
     CDS             14703..16052
                     /gene="legY"
                     /locus_tag="ACF2SP_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegY"
                     /protein_id="HFD2357955.1"
                     /translation="MATFFFKTNYYQEIMLKRIFFLMIFFVSQTMASVFTHEEVQILK
                     KHFLNNFQTNGAIVASPSQYNPNYYYDWIRDSAIAMGLVETWYEASQSARYKKLLLEY
                     VSWTEKIQHQADPIAGQDILGEPKFYINGNPFDGEWGRPQNDGPALRASVLIRFAQQL
                     LDHNEIDYVKSHLYNNTMDPQSMGTIKMDLEYIAHHWQDANFDLWEEVYGHHFFTAMT
                     QQKALTDGAILAHQLHDRQAAVFYEMQANLINSRLQQHLDHQNKIIQATLLPHPGPQK
                     TLELDSSVMLGILTNPQKEGVFAPHHTFVRNTAKALHEQFNLMFPINKNRSGAILFGR
                     YPGDTYDGYQTNSIGNPWFILTATMAEYYFTMAHNLSLNSSNKLHIQNYLKKGDNYLR
                     LIKQYGPDLNLSEQINLNTGVQQGATSLTWSYVSVLRAIHLREQLENRIKTMGWNTY"
     gene            complement(16142..17563)
                     /locus_tag="ACF2SP_000140"
     CDS             complement(16142..17563)
                     /locus_tag="ACF2SP_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFD2357956.1"
                     /translation="MAWIVAIIGSIAGFLFGYDEGIIAGSLGLVKNHFNLNATHIGVM
                     ASALPFGALFGSLLIGAFMASKSVKRFGRRSLLSFAGFLFFVGALGAGFAETISMLIL
                     SRLILGLAIGMASVLTPLYLAETAAMRSRGAVVAIYQLALTVGIVCSYSVNYLLIEQQ
                     AWRAMFASSAIPALLLTLGILFMPESPRWLCSVGRHDAAANSLRKLRGKQPVEQELKD
                     IEATLANEPKQGNWLLLFQKPLLPVLMLGTILFCLQQLSGINVVIYFAPEIFKNLGLG
                     STTGQILATMGIGLVNLLVTIIAILYVDKLGRRKLLLLGFAGTSLSLFALSLFSLNHV
                     AWLSYLSVICLMVYIFSFAISVGPIPHIAMAEIFPLHVRGAGMGMSSMSNWSFNTIVI
                     FSFPVLHQMFGIEVTFVLYAVICFLGFIYAYIYMPETRNISLEQIETYIMSGKPLRFL
                     GREDEEVNAVSTKSESSLLASTN"
     gene            complement(17677..18339)
                     /locus_tag="ACF2SP_000141"
     CDS             complement(17677..18339)
                     /locus_tag="ACF2SP_000141"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFD2357957.1"
                     /translation="MSFSEWKTQPGAVFAASPVIPVIVIKELGDVLPLAEALFAGGIH
                     VLEVTLRTPVAIKALELLINTFPDELIGAGTVITPGQLHDVAAAGARFAISPGQTREL
                     LIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKF
                     CPTGGINSKNYEEYLCLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCS
                     QN"
     gene            complement(18326..19321)
                     /gene="glk"
                     /locus_tag="ACF2SP_000142"
     CDS             complement(18326..19321)
                     /gene="glk"
                     /locus_tag="ACF2SP_000142"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFD2357958.1"
                     /translation="MMPHDLAQYAIVADIGGTFARFSRVNLVNLQMDKIEISPCAEFI
                     SLESALLTYKTRHSLQEIKHIAIAIACPVIDDLVSMTNCHWQFSITELKQRLGLEVLE
                     VMNDFTAIAMSLPVLSTQDLVQIGNGYLDASKVRVVLGAGTGLGVAYLIPHQHHYSAF
                     AGEGGHADWGAKTEQEWFIYRYLKSKYSHVSYERLLSGQGLENLYQALAAYHSKKVEF
                     LSAAQIISLALNQECFIAHKAVAQFFSSLGSFAGDLALTYGAFGGVYIAGGIMPRLLS
                     LVHQSDFRIQFEDKGRFSDFNALIPTYVIAAAQPGILGASVSLKQILTGASHVLF"
     gene            complement(19308..21146)
                     /gene="edd"
                     /locus_tag="ACF2SP_000143"
     CDS             complement(19308..21146)
                     /gene="edd"
                     /locus_tag="ACF2SP_000143"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFD2357959.1"
                     /translation="MHPVINQVTENIQNRSAARRAAYLNQIDKARTKGPQRSILHCGN
                     LAHGFAACAKQDKAILRGNAKANIAIVSAYNDMLSAHQPYSTFPDLIKEAISAAGGVA
                     QFAGGVPAMCDGITQGQPGMELSLLSRDVIALSTAVALSHNMFDGGLLLGICDKIVPG
                     LLMAALAFGHLPFIFIPAGPMPSGISNQEKARIRQLYAAGQVDKNALLDVEAASYHTA
                     GTCTFYGTANSNQLVIELMGLQLPGSSFVNPNTPLRNELTKAAACQILSLTDLNSDYM
                     PIGQMLDVKNIVNGIIGLLASGGSTNHTMHLVAIAAMAGYWVNWDDFAQLSAIIPLIA
                     RIYPNGQADINHFQRAGGMAYFVKTLLEGELLHPDVNTVVGFGLDKYTQQPQLIDNRV
                     VWVNGPDSSSNNDVLTSAADPFKPQGGLQVLSGNLGRAIIKTSSLYDEHYVIEAPAVV
                     CSSQEEFEHLFNQGLLNKNCVVVVRFQGPKACGMPELHQLTPKLGVLMDKGFHVALVT
                     DGRMSGASGKVPAAIHVTPEAVDGGGIAKIETGDMILIDCERGILQLLVSDEELAGRS
                     PVAIEGYSSVLGMGRELFGNLRLHFTGAEQGACSLFKGNEFFYDAS"
     gene            complement(21134..21847)
                     /gene="pgl"
                     /locus_tag="ACF2SP_000144"
     CDS             complement(21134..21847)
                     /gene="pgl"
                     /locus_tag="ACF2SP_000144"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFD2357960.1"
                     /translation="MQFHSFRDAKQLIHDMVEKLRTILSDAIKRRGQAFLVVSGGRTP
                     IDLFKELALADLPWEKVVITLTDERCVNLFDPHRNELLVREFLLQEKAKKAKFISLFD
                     EKEDPLGHLNEVNSQIDSLPTFDAVVLGMGEDGHTASLFPCSDELSAGLDDKANAVLI
                     VKPRTASFQRVSLSKRRLLNSSTIFLHLVGQRKQVVLEQAMATNNPKILPICAILNAP
                     DANVQVMYAPNNEEKECIL"
     gene            complement(21834..23324)
                     /gene="zwf"
                     /locus_tag="ACF2SP_000145"
     CDS             complement(21834..23324)
                     /gene="zwf"
                     /locus_tag="ACF2SP_000145"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFD2357961.1"
                     /translation="MLLKAESKYPCDLILFGTLGDLSCRKLLPALYQLELANLLHEKT
                     RIIGCAREELDFSAYADLIKNKIQQFLFDEIDEAVWLRFIKKLAYCKLDLNQSDDYQK
                     LTGYVNPSERVVISYLAIPPSLYSQTCRSLARLNLTQKPSRVVLEKPIGHDLPSSVAT
                     NEQVACFFSEDQIYRIDHYLGKETVLNLLVLRFANSIFSTNWDNRVIDRVEISVAEEI
                     GVEGRWEYYDKSGQVRDMLQNHLLQILSLVAMEPPLSLSAESIRSEKLKVLKSLRFIN
                     KSNAHDHTVRAQYVANNLANGKKIPGYLEEVGANQASTTETFVAIKAYVDNWRWSGVP
                     FYMLTGKRLPKKQSEVVIYFKPQPYNIFHPLNEELYPNQLIIRLQPDEGVEVRIMNKI
                     PGLGERMMLHDSKLDLNFHHSFKTQRIADAYERLLLEVMLGNQYLFVSREEIEQAWQW
                     IDSIVSAWEEDNIPLYTYPACTWGPKEVIRLFNGQAHVWGDHNAVS"
     gene            23640..23885
                     /locus_tag="ACF2SP_000146"
     CDS             23640..23885
                     /locus_tag="ACF2SP_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357962.1"
                     /translation="MLNKRFSERLNKELDNIGVPEATAERIEILSKLIKIPKFKAEAL
                     LNGITSPDEAVLKTLAQELEVNADWLIGKSDSKSGAH"
     gene            complement(23889..24797)
                     /gene="rimK"
                     /locus_tag="ACF2SP_000147"
     CDS             complement(23889..24797)
                     /gene="rimK"
                     /locus_tag="ACF2SP_000147"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFD2357963.1"
                     /translation="MKIAILATNPHLYSHKRLKAEAEAAGHEVKIINPLYCYMNVAAS
                     NPKVHYRGGAPLPHFDAVIPRIGASITYYGTAVLRHMETMGMYTLNESIAISRSRDKF
                     RSLQLLARKGIPMPLTSFAQSPDDTEDLIHMVGGAPLVIKLLEGTQGKGVILADSHQS
                     AVSIINAFKEMHANILVQEFIEESRGTDIRCFVIGEKVVAAVKRQAKDGEFRANVHQG
                     GKAVKVKLSPQERAIAVSAAKTMGLRVAGVDLIRSNHGPLVLEINSSPGLEGIEKATN
                     INLAGKIIEYIEKKAKPISSNHRFHG"
     gene            complement(25163..25804)
                     /locus_tag="ACF2SP_000148"
     CDS             complement(25163..25804)
                     /locus_tag="ACF2SP_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFD2357964.1"
                     /translation="MNIKIKGGAVTITVLALVSLLAGIYISQNFHFKKKINVADFHGT
                     YLENPRTVNNFSLTGTDQKAFNNSSLQGRWTLVFFGFTNCGYLCPTTMAELGKMYRIL
                     EKKGVKNLPQVVMISIDPQRDSLDKLGSYVTSFHKSFYGARGEEDSIKSMTHEMGIAY
                     AKINNKESSEPENYDIQHSGAVMLFNPQGELNAFFTTPHHADLLAKDYVLLVS"
     gene            complement(25814..26698)
                     /gene="cyoE"
                     /locus_tag="ACF2SP_000149"
     CDS             complement(25814..26698)
                     /gene="cyoE"
                     /locus_tag="ACF2SP_000149"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFD2357965.1"
                     /translation="MRTEYAARLPVDWRDYVELCKPRVVLLMLLTVIVGMYLAAPGWV
                     SLRLIAFTLLGIGLCAGSAAAINHLVDRHIDSIMARTKKRPVAYGRVSVKQALWFAVI
                     IGLMGLSLLILFVNQLTALLTFVTLIGYAGVYTGYLKRATSQNIVIGGLAGAAPPLLG
                     WTAVTGQLDPQALLLVLIIFTWTPPHFWALAIYRYKEYQDAEIPMLPVTHGIQFTKLN
                     IYLYTVLLLVVSLLPFVVSMSGWIYLLGALVLGIRFLVWAHKLYFTDKPVVAMQTFRF
                     SILYLMLLFVFLLVDHYF"
     gene            complement(26688..27695)
                     /locus_tag="ACF2SP_000150"
     CDS             complement(26688..27695)
                     /locus_tag="ACF2SP_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HFD2357966.1"
                     /translation="MSNSSIRLAATLAVFLAFFVVMLGAYTRLTDAGLGCPDWPGCYG
                     RMVLPGADKGLQEAQSQYPDLPIESKKAWTEMAHRYAAGTLALLIFFIAFQVWFKRRQ
                     DQDLPWHLPLALFMLVVFQAALGMWTVTLKLLPVVVMGHLLGGMLIVACLCRFRLQIA
                     QLKGQNLPEWRPWLRLGIVIILLQIALGGWVSANYAGISCIGFPLCNGTWFPELHFAK
                     GFNLFSTIGANYQGGILESEVRASIQFIHRIGAILTATYLLTLSSIIVFKTHSIYLRA
                     TAIIMAMLVLLQFTLGIMNVIHLLPLKVAVAHNGVAALLLAVAFCSLHFTQDKEVSHA
                     Y"
     gene            complement(27700..28245)
                     /locus_tag="ACF2SP_000151"
     CDS             complement(27700..28245)
                     /locus_tag="ACF2SP_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357967.1"
                     /translation="MKKPYKYMTLLLLVVIFAAPGITAYLFYKNPSWLGTSKVNKGIL
                     LSPPIALKPLDSSAKWRIIYWNPNGCDKTCLKQLNMLGRVRLSFGRKLYQVDEWLLLS
                     NKDSLIAEELKPILKEQDIHVAALSLTEMSKLSTLYPEAKIFIANPDNYLILSYQPGC
                     NPGDIYKDLKLLLNTTEIKSG"
     gene            complement(28229..28957)
                     /locus_tag="ACF2SP_000152"
     CDS             complement(28229..28957)
                     /locus_tag="ACF2SP_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HFD2357968.1"
                     /translation="MPSLTCFNFRFTSNWPMLILTAVCFFLFISLGFWQIHRADEKIE
                     MITAQQALAKQEPITWQPGQKLPEQYQRISIEGAFLPKLFLLDNQHYQHQFGYDVVSP
                     MLLDDDSIVMVDRGWVSGDITRRTFPNVQTPNGKFKLFGMVYFPSKKQWVLGPSFEEK
                     ENKVTLLELIDEEILKQLLQKKVYPFIIRLDKNEPFGFVREWEIVSMPPQRHFAYAIQ
                     WFAMALVILIIFVIFNLKKNEKTI"
     gene            29025..29228
                     /locus_tag="ACF2SP_000153"
     CDS             29025..29228
                     /locus_tag="ACF2SP_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HFD2357969.1"
                     /translation="MFTKIFIIFVMILIVASLASGLFYLVKDTDKSKRTVKALTVRIA
                     ISLSLFVFLFIAFKLGLIQPHGI"
     gene            29505..29648
                     /locus_tag="ACF2SP_000154"
     CDS             29505..29648
                     /locus_tag="ACF2SP_000154"
                     /inference="COORDINATES: protein motif:HMM:NF039621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5993 family protein"
                     /protein_id="HFD2357970.1"
                     /translation="MIVLFLFYFIIGLQIIFKPNKTIILQFLFSLFLILISFNYHSHL
                     VNL"
     gene            29645..30193
                     /locus_tag="ACF2SP_000155"
     CDS             29645..30193
                     /locus_tag="ACF2SP_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFD2357971.1"
                     /translation="MNKQMQWHLFGNTSGLLIICCLLILAFLDQFIFYELPCPICILQ
                     RICFAAVGLCFVMNLMLGIRASHYGLMLLASLLGLSISVRQMYIHLTPGDPGYGGLFL
                     GLHLYTWSAIIFLVIILLIAGALILDKGLMPDYKVRSKSAKVMIWVFFILILANGIST
                     FLECGPHECPDNPSKYYLLNNN"
     gene            30212..30655
                     /locus_tag="ACF2SP_000156"
     CDS             30212..30655
                     /locus_tag="ACF2SP_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFD2357972.1"
                     /translation="MRLLFISLILASGYLQADTINNYMNIANNIPQMEMKADPQAQAW
                     ARSARHVLTITSESIAETLIQANEAAKTQGKPIFCLPQGVQLNSFVMNELIQQTYKEI
                     SSQQSDKDKMTVSQVALLGLSKKYPCEPSPQEKQIQHVASLLSPQ"
     gene            complement(30869..31210)
                     /locus_tag="ACF2SP_000157"
     CDS             complement(30869..31210)
                     /locus_tag="ACF2SP_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFD2357973.1"
                     /translation="MSDKFSHITKDITTQLAKFRKEMPELMTGFSSLAQAATKDGALD
                     KKTKELIAMALAVAKQCPGCIGFHSQTLVKLQATREELLETLGMAVYMGGGPSLMYAA
                     EALEAFEEFSK"
     gene            complement(31414..32004)
                     /locus_tag="ACF2SP_000158"
     CDS             complement(31414..32004)
                     /locus_tag="ACF2SP_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0405 family Dot/Icm T4SS effector"
                     /protein_id="HFD2357974.1"
                     /translation="MLYNLARQVKFNNPSCLCNFQKMGYIDELLEELSARLPELEWKI
                     NGLSSSISIHNLPKGIFSSVIEFNGPACIKEIRDDIHALQKHKDESIACFLAERIQKK
                     INVLVILCQMDKKNNKPESKISFDLTTLSTRQQWIKTMEENICALEEQHQAMRKTLEQ
                     MKSCSNPATILHLQTELGNVEKRLTLAKETLNRAIS"
     gene            complement(31994..33397)
                     /locus_tag="ACF2SP_000159"
     CDS             complement(31994..33397)
                     /locus_tag="ACF2SP_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD2357975.1"
                     /translation="MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILG
                     ALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILS
                     FVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
                     MSLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHAN
                     DVKDPQHAFPKALVYSVGIILSTLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYH
                     MDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLY
                     TQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPQHPRP
                     FKIPGGMAGMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPP
                     FITSWLQAKEADLEPAV"
     gene            complement(33734..35242)
                     /gene="ankG"
                     /locus_tag="ACF2SP_000160"
     CDS             complement(33734..35242)
                     /gene="ankG"
                     /locus_tag="ACF2SP_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkG/AnkZ/LegA7"
                     /protein_id="HFD2357976.1"
                     /translation="MIYVLICIMLVCGLVVYQLMPKLLSYSTSLISKKQDTSELTHDG
                     ITNVLTQLGHPKFEGVCYGFTLNWALAVAQGKESFFYRQLHHLRTHQFGLPKTLQQIK
                     EKKERNQSLSNDEMVIDTLPQLGKKICVAQDPLQYKEKYRKLVWQPDINSILKAINAD
                     SSVAKHIFYKTHSFLNQDEATEYLELLKRTGIREDVAVIISTADHAMGFKLAGNVWRF
                     ININDLYQQDKNKPYFEFSSRNLVKELYRVCAENLQGSRLTVNTDFVSVNPEEKLSRA
                     LQNVFPVFPVRTKTSYPERLAFFSMAATQGDMNSVKKCIHSGWSIFSRQRLSDDSPIL
                     TAIYLGRRDVVRAMLSTSRHRVNQKRKSDSSTLLHIACRYGGSGIVEDLLNIRGIKID
                     PQDNKGRTPLMYACKKSVVTEDKKLFNLLFAKGASLSIKDNDGLTALDHALKNEHTLA
                     IQMIEERLEKEACAKENSTSRRFKFSETKGTLFQRGVKTISYQSQQPGFGMK"
     gene            35504..36178
                     /locus_tag="ACF2SP_000161"
     CDS             35504..36178
                     /locus_tag="ACF2SP_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357977.1"
                     /translation="MESLMPKAFTHLCKKVNLSLYNDTLTVYPKSSVLPKPIQIRSEL
                     DLLMVLLFSQMDCNSAQVDQFFMMRSNELGVYRFIHRDDTDHDLYHQDEFSMDDNHYH
                     AQFDKAIDEIKLENILGILEKHSLISSEEHVSFIEAYHKANTLTEDMPKLETTLSSPL
                     PISSPSVHSGAKRPVVKEKEVDTPTTFAGLRVGFFLTSTKTTTKPLSRSHDDEKKIGT
                     NRHEFN"
     gene            36435..37811
                     /gene="ffh"
                     /locus_tag="ACF2SP_000162"
     CDS             36435..37811
                     /gene="ffh"
                     /locus_tag="ACF2SP_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFD2357978.1"
                     /translation="MFENLTERLTRTFKNLRGQGRLTEDNIQHALREVRLSLIEADVA
                     LPVIKEFIEQVKQKSLGQEVLTSLNPDQAFIKIVHDELIHVMGDERVELDYKTQPPAV
                     FLMAGLQGSGKTTSTAKLARYLKETENKKVMMASVDVYRPAAIHQLKVLAEQIDVAFF
                     DSDPNEQPIKIAQKALESARKQYVDVLILDTAGRLHVDEDMMSEIKSLHKAVNPIETL
                     FVVDSMTGQDAANTAKAFHEALPLTGVILTKTDGDARGGAALSVKQITGKPIKFIGSG
                     EKIEALEPFHPERIASRILGMGDILTLIEEVERKADKQASEKLAKKLKKGKGFDLEDF
                     KQQLLQMNNMGGISGMMSKLPGMGQLPKQAMQQVNDKAMAQTIAIINSMTPKERRIPK
                     IIVGSRKKRIALGSGTQIQDVNRLLKQFEQMQKMMKKFTKPGGMQKMMRGIGGMAGLK
                     GMFPDDLK"
     gene            37898..38158
                     /gene="rpsP"
                     /locus_tag="ACF2SP_000163"
     CDS             37898..38158
                     /gene="rpsP"
                     /locus_tag="ACF2SP_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFD2357979.1"
                     /translation="MVVIRLSRAGAKKRPFYHMVVTDSRKRRDGNYIERIGYFNPVAR
                     GQEVKLHIDMDKMTHWQKVGAQLSDRVSALLKEHSKKSETAA"
     gene            38164..38673
                     /gene="rimM"
                     /locus_tag="ACF2SP_000164"
     CDS             38164..38673
                     /gene="rimM"
                     /locus_tag="ACF2SP_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213051.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFD2357980.1"
                     /translation="MNNKTNWVIIGRFGRPHGIKGFVTVHSFTDPADNILRYNDWHVF
                     LNKQWQPLKLLTIEVRSKAIIAQIEGYPERELVSALTNLDIGVQESQLAALAPGEYYW
                     YQLIGMSVINSKGDLFGKVVEIMPTGSNDVLVVEGEKRHLIPYLPGQFVINIDESQQV
                     ITVDWDMNF"
     gene            38673..39425
                     /gene="trmD"
                     /locus_tag="ACF2SP_000165"
     CDS             38673..39425
                     /gene="trmD"
                     /locus_tag="ACF2SP_000165"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFD2357981.1"
                     /translation="MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSS
                     RPYKQVDDKPYGGGPGMVMMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQ
                     IAIQKQSLLFVAGRYEGIDERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
                     SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKT
                     WLKRPDLLEKIQLSETDKQLLAEFKCEHGDSC"
     gene            39452..39817
                     /gene="rplS"
                     /locus_tag="ACF2SP_000166"
     CDS             39452..39817
                     /gene="rplS"
                     /locus_tag="ACF2SP_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFD2357982.1"
                     /translation="MTNIIDQINAEQMQGKEIPDFNPGDTVLVQVKVIEGNRERLQAF
                     EGVVIAKRNRGLNSAFTVRKISHNVGVERVFQTYSPIVDSITVKRRGDVRRAKLYYLR
                     NLAGRAARIKEKLSGKKGD"
     gene            39833..40288
                     /locus_tag="ACF2SP_000167"
     CDS             39833..40288
                     /locus_tag="ACF2SP_000167"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFD2357983.1"
                     /translation="MLLVSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLI
                     ASELKAYFDDPHHRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRA
                     VGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKMI"
     gene            complement(40389..41252)
                     /locus_tag="ACF2SP_000168"
     CDS             complement(40389..41252)
                     /locus_tag="ACF2SP_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0393 family guanine-nucleotide exchange
                     effector"
                     /protein_id="HFD2357984.1"
                     /translation="MLSSTKEYLQALRDGKYLLFLQWPKFIAEYYGKNDKNQEADEMV
                     TLLIFEWLNNGFCLDDIKKFAILYAVHEMESRPLREGLSYALTTISIALFPCMVYLTN
                     NLQEHYITSKKLSSKEVLQLMTMNNAYLEKQRFVEFLGQEQDKFFTWVKEADSSAVSK
                     AFDQIYSVTYLKYLIEDYLSLLESANLPNDQLKSSRISLVVRLAKYLHEQTELTQDVH
                     DEIAVYVKKLWEMQPAEFEEEFLKKISPLPFIDNTVRILTGLSARFFSILQPPSPPLN
                     INSDTDKNPKI"
     gene            41401..41682
                     /locus_tag="ACF2SP_000169"
     CDS             41401..41682
                     /locus_tag="ACF2SP_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357985.1"
                     /translation="MKNKLAISILLFLLPTAAAMANCDLTKFRWDCDLPVQVKPSPGA
                     SSLVYCGSSYGYITKQQYDILARYQRASVNMVLDINGEYIDSPCIGAER"
     gene            41869..42576
                     /locus_tag="ACF2SP_000170"
     CDS             41869..42576
                     /locus_tag="ACF2SP_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFD2357986.1"
                     /translation="MHIQIDGIPIEILRKPIKNLNLRIYPPDGLVKVSVPLKYSEQLI
                     KQQLQEKSEWIRAQCERIRARSFYREPSLQTGATIPFKGKNYLLIVEEHHGPSQVLIK
                     DELIYCYTKPDLTPQQKQNILERWYRKEMNLVIPRLIQQWEAIMKVKINQWGIKKMKT
                     RWGSCNPRAQRIWLNLNLIKKPLECLEYVLVHEMVHILEASHNKRFYALMAQFMPKWQ
                     EYQRILEGTPNANTFAR"
     gene            42662..43300
                     /locus_tag="ACF2SP_000171"
     CDS             42662..43300
                     /locus_tag="ACF2SP_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HFD2357987.1"
                     /translation="MFNPDLIAHNICTQGFHIIDEFLEEAHYYSLRDMAYELKQQNLF
                     RNAKIGQNLQAQQNDKIRSDQIFWIDADSPYLPIQAYLKKLISLGNVLNQTMYLGLAE
                     FETHFAIYLPGTFYRKHIDQFESTKTRKISFVYYLNDTWQKEFGGELLLYNIEDELIQ
                     NISPLGNRFVCFDSQLPHEVHPTQRTRFSMTGWMKTRSTSVLADNKLTQITP"
     gene            43387..44445
                     /gene="vipA"
                     /locus_tag="ACF2SP_000172"
     CDS             43387..44445
                     /gene="vipA"
                     /locus_tag="ACF2SP_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipA"
                     /protein_id="HFD2357988.1"
                     /translation="MKHYDSMDQELITMPISNAFLKLFSEFVDNYARNGTPSFYHWAK
                     EQNHEVINKFFHELLEIIKSNTETFHDVSIIEHLNATVLLSAKTNPYLLDCWISFITK
                     YNAETNSFTPIGASMHIKVGPEGTPSERIIRELKESLVMTQQAFILAQKANATEVDSL
                     KRQLEQTKQENERLVTQNQFLKDQVSAEKTFADLNPQINNARQQLSALTELIQTLSEI
                     TNAKPKIETVSSLSIPAVTESKSELLKENSCSVAPVSKEVTPAISQEPKKRPVLPPPN
                     DMDGQALSPNIPPKEDVSIKVTIPSMPKPNSTQPSAKNSGFFSGDGFLKELNAKIKER
                     KERPKDEPHTTTVEKKNL"
     gene            44676..45590
                     /locus_tag="ACF2SP_000173"
     CDS             44676..45590
                     /locus_tag="ACF2SP_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2357989.1"
                     /translation="MHALEIKQLSKTYSNGVEALKGIDLTVNTGDFFALLGANGAGKS
                     TTIGLITSLVNKTSGNIKIYGFDLDAYPEKAKSCLGLVPQEVNLNIFENCEQILLNQA
                     GYYGISRKKALPRAEALLEQLGLWDKRRSIVRHLSGGMKRRLMIARALIHQPKLLILD
                     EPTAGVDIEIRHSMWDFLARTNAEGTTIILTTHYLEEAEQLCKNIAIIDKGQIIKNTS
                     MKALLQTLRHQTFIFNTENPIDYLPDIHPFNVTPIDPTTFELRVDNNFSLNEVFAVLT
                     KQGIKIHSMRNKTNRLEELFMDLIKNGI"
     gene            45580..46353
                     /locus_tag="ACF2SP_000174"
     CDS             45580..46353
                     /locus_tag="ACF2SP_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2357990.1"
                     /translation="MGFKYQLIALYTLVRREVVRMFRISSQVFLPPVITTTLYFLIFG
                     SLIGPRIGDIEGVDYSQFIAPGLIMMSVIINSYGNVSNSLFSVRFQKSIEEMLISPMH
                     NGLLLLGYVIGGVLRGLIVAILVFVISTFFLPVELDHLPMTLLVVVLVAAIFSLAGFT
                     NAMVARNFDDIMLIPTFILTPLTYLGGVFYSINMLPPFWQKISLFNPILYMVNALRHT
                     MIDQEEVSITLAMIIIFLMLIALTVGNMILLKKGVGFRE"
     gene            complement(46380..47399)
                     /gene="htpX"
                     /locus_tag="ACF2SP_000175"
     CDS             complement(46380..47399)
                     /gene="htpX"
                     /locus_tag="ACF2SP_000175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HFD2357991.1"
                     /translation="MGIADYHATAGDWRAQLRRNEKKTRRVIAVFFLVYIFVGLIVDA
                     FVLQGMYPHASLENIIHALITFKVVPIATLLMIAFAGISLLITYALYDRIMLLGTEYY
                     QITPETARSLQEKQLYNVVEEMKVASGLQYMPKVYIIEASYMNAFASGYSEKSAMVAI
                     TRGLMEKLDRAEMQAVMAHELSHIRHHDIKLTLMVAVLSNILLIVIDILFYSVIYKRD
                     RRGDNRLLMVIIILRYVLPIITVLLALFLSRTREYMADAGCVELMRDNEPMARALLKI
                     NQDHVQNAEQYAKAYGDTPHEQVRQASYLFDPSHIDPVKSLNNAFSTHPSIEQRLEAL
                     GFKRK"
     gene            complement(47410..47991)
                     /locus_tag="ACF2SP_000176"
     CDS             complement(47410..47991)
                     /locus_tag="ACF2SP_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFD2357992.1"
                     /translation="MKTFFIAILIIIALAFVWAISIYNTLIGLIEAINNNKKQIDIQL
                     DRRFKVFESLIEAVKKYMDYEKTTLKDVVALRNQAQAAKDAGDEQGRIAAENGISKIA
                     SNLNLVFEQYPDLKASQNVMQLQEEIVNTENKLAYAKQAYNDGIERYNAKKKSFFESM
                     VVAMFSSALDKEFIYWGLPEEQIQAKENYTVKL"
     gene            complement(48174..51029)
                     /gene="uvrA"
                     /locus_tag="ACF2SP_000177"
     CDS             complement(48174..51029)
                     /gene="uvrA"
                     /locus_tag="ACF2SP_000177"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFD2357993.1"
                     /translation="MHTITIRGARTHNLKNLDLELPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDSIEGLSPAISIEQKATSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPTHHTSLHAQTISQMVDQVLALPEDSKVMILAPVVRERKG
                     EHVQFLQQLQAQGYVRARIDGELYELDSPPKLGLRTKHTIEVVIDRFKVRPDLAQRLS
                     ESFENALNLADGLAIVSSMDGHFKELLFSSRFACSECGYSLSELEPRLFSFNNPVGAC
                     SSCDGLGVDQFFDPSRVVHDATASLAEGAIRGWDRKTTYYFSMLESLARHYGFDTSTP
                     FCDLSEAMQQIVLYGSGREIIEFQYHRPHGGYMVKRHSFEGIIPNMQRRYRESDSGMI
                     REELAKYLSSRPCQSCQGARLREEARHVFIDNKNLPEITSYSIEQAYQFFKELKLSGY
                     RGEIAAKINKEIVERLGFLVNVGLDYLSLARSAETLSGGEAQRIRLASQIGSGLVGVM
                     YILDEPSIGLHQRDNDRLLSTLMHLRNQGNTVIVVEHDEDAIRSADFVLDIGPGAGVH
                     GGEVVASGSPEEVMKNPASLTGQYLSGKKEIQIPKNRIPVNHDRMIHLKGVSCNNLQN
                     VDVSIPLGLLTCITGVSGSGKSSLINDTLYPIAANALNRASLMTIGEVKDISGLHLCD
                     KVIDIDQSPIGRTPRSNPATYTGLFTHIRDLFSGTPESRARGYQPGRFSFNIKGGRCE
                     ACQGDGLIKVEMHFLPDIYVSCDVCQGKRYNRETLEIHYKGKNIHEVLDMTVEDACAF
                     FAAIPVIARKCQTLMDVGLSYIKLGQSATTLSGGEAQRIKLARELSKRDTGNTLYILD
                     EPTTGLHFHDTKQLLTVLTRLREQGNTIVIIEHNLDVIKTADWIVDLGPEGGSKGGKI
                     IATGTPEMVAENSRSFTGQFLKQILSKSNELLLQKCK"
     gene            51124..51582
                     /locus_tag="ACF2SP_000178"
     CDS             51124..51582
                     /locus_tag="ACF2SP_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357994.1"
                     /translation="MKSLIKSLLFIVAFSINSSALYAANSNTRVIQWAQQTLIDTLSV
                     SYTTKSQDFATVRKNYSLNAWSGITGFLGGNMQSIREKQLTLHPVLLPDTQILSTGLY
                     LGMSYWIIRQSVAIDELNIKLTFTLTILGRSGAAGYPYIVQSLSILLNAL"
     gene            51841..52392
                     /locus_tag="ACF2SP_000179"
     CDS             51841..52392
                     /locus_tag="ACF2SP_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFD2357995.1"
                     /translation="MPKCMHFILATALIFVTSTFYASNLQEIEQEVSDSVITAKITAK
                     FAESRHLNPLKISIDTEKGIVTLSGYAPDKQAFIDALQIAKDTKGVKAVHAKDLHIKK
                     VNTAFTDAFITAEVETAVLKAKILDDESIPLVGINASTTNGEVTLSGHVKSAGSIAAI
                     IKRVNEIPGVKKIVTNLEIQENA"
     gene            52389..>52861
                     /locus_tag="ACF2SP_000180"
     CDS             52389..>52861
                     /locus_tag="ACF2SP_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HFD2357996.1"
                     /translation="MSPLTQFKFFLWFFCHFKVALIGYLKPKLIQLTDNEIVVRLPLT
                     RRSRNHLHSMYFGALAVGADIAGGLHGFYHAKQAKCKVSLAFKSFQAQFLRRPESDVY
                     FICTEGEVVKKMIEESKKSGERINKPIHIKAYINYLTHPEEIADFILELSLKVIK"
BASE COUNT        17097 a        10827 c         9416 g        15521 t
ORIGIN      
        1 atacaatatc atcttgaaag ctattatcaa attaaaagtt gccatattca atgacaaact
       61 gtaaaaccag tgacactttg ctattaaaac ccattctggg atatagttgt tttaattttt
      121 ctataagtgt ctttatttta attttgtttt ctatcctgat aacatcaggc acatgttggg
      181 caaaaaaaag tgtaaatcac aatgaggttg taatgagagg aaaaagagaa tttttaaaac
      241 atgaatactc ccctggccat tggtcaattg attatactcg ggcaggcacc tcaatcgcag
      301 ttatcactgt ccgaaataaa tatcattact ctgtgattct taaccctacc gattgcagag
      361 gctatcgtat cattattcga tatctgaatg agggggattc aagcctttca tctgctttta
      421 accgccctta tactgtatct gaacaacgcg gattgaatga cgttgcaagt ttgatgaccc
      481 aagtctatga aaaattaggg cttattgtgc aattttctca acttggcaat aactcccagt
      541 ctttcgataa agagacagga gtaacgctca ttggaagtga agaagaaccc agtatgcttc
      601 atttgcatat gtggggcagg ggcgatcctg acatggaata tatcgccggt gttcctctta
      661 gaggacccga acctggtttg atgtttgatt taattgcaaa gaataagact caccctatta
      721 accaacatgc cataaaatgg aatgacgagg agctcaaagc ctgtcttgcc atgttcaagc
      781 ttaaactggc tgaatacgtt aattcaccag aatttactga agaatttggc gataccctaa
      841 aagtgactat tcacgacaaa aaagatcttg gcgaagagaa ggaaagtggt tttgggtttt
      901 tccataaaat gaaatctgct tcagatggta taacaacttt ggaaaaaacc ttagccctgg
      961 aagataacaa aataaatccc ttcgtataat cgacagagca atataaatga tatcgtcttt
     1021 attgtactcg aatttcagcc aggaaaagta tcaaaatttc cggataaaaa tgttttcagc
     1081 cagcgactat taaccatacg catccactgt ctacctgtta ctactagcaa cttgatagtg
     1141 gataatctta tgaaacacat atctgcagat tttttatctg ttcgaattca atttatcaac
     1201 agctcaattg atggtcatca aaaaaactga tgaatatgtt tacagcactt gtatacaccc
     1261 tacaagagtg gcatttattg gcattaagcc tctggcataa cctgtttatt ttatttcagg
     1321 acaaggggca aacatcttta aataagcacc acagaaaagt tcgcaacgcg gcagtaaaat
     1381 aaaaataaca ggttatgcaa agcagtctat tcgtctcggc aaagagtgca tcaacacata
     1441 caacgaagta caagctatag gtctattatg aaagccacgg aacctatgga gaagagaagt
     1501 tgcttgtctt taaaaaaatt taaaaacgag ctagacaaag aacaggactt acgaaaaact
     1561 ccaaggtcaa ggcaaaaaat gtttttaatg agggagttta gatagactaa atgaccgaat
     1621 taacaacatt ttttaacata gagattgggg tttttcgtaa gtcctgtaga aattatattt
     1681 gagtgcaaaa atcaatcttg ctcagggtga tttattcgaa aagatatatt cttaatgcaa
     1741 aatttccctg aatttgatgc ctcgggcaag ccgaggctag tagagaatgg gtgggatctc
     1801 aaattatcct ttagcaccca taaacagttc catcttttgg cgtatggtca tttttcttat
     1861 ctccactctc ttcgtctttc ttgctgaaga atgaatttga tttgagtgct tccttaacat
     1921 gcttcttatt cacaagagcc tgagtatacg tggttgcatc attcatcgca tgctgcagga
     1981 atccagcaag ctcagggttt tctttaaaat agctataaag gttaacttga acaccatatc
     2041 ctttgaatat gtcttgtaca taaggatcat gaagaacatc aatgacctca agcatggcat
     2101 gaaaaccgcg cttggcataa acacctatga cctgaataag aaaaagattg atatcgtttt
     2161 gcagatcagg atcttcctta taattttcca tatacttggc taacataccc acaatattac
     2221 aagtatgtcc ggaaatcgat cctacgtaag acgaatactg acggttatta ttccaaaacc
     2281 attgttttcc ctctttgtta tcaggagcaa cagttgaaga atccactagc atattacgca
     2341 tgggttcgcg ttgggcatca taaaagacag gcgaacagcc tttcatgact ccaccgccac
     2401 aattccatac atccgaacct actttttctg aatcagtctc aaattttctt tctttaatgc
     2461 cagtcaactt gtcacgttca acccgatcgc gccctttgtg cgcttcagca tgatgtaata
     2521 caccggtaat aaattcaact tctttcttgg tgaatttaga ggtatcctta tagccattgg
     2581 caaagataca aattttttgt tccatttctc gtggagtatc cactcttcca caatcaccta
     2641 aaaagaattg cagaatttta ttacatgctt tcagtgtgtt atcccttaac tcctcatctt
     2701 ccggctccag actctcttct gttaaatcga tttcaagggt tttttcagag tgggacagcc
     2761 cattaatata ctgagcaagg cgagtttcaa aaaatcgtct tgggtcatta cgtgtcggtt
     2821 taaaccgctt aaggtttcca gaagattcca tgattttttg aacttgtttt tcccctaatt
     2881 gttctactac ttctttttcc tttagttttt ctattatttt atgcaagcag tcatcatatc
     2941 gaacaaataa ataatcagcc gataattctt gcaaaaaccc cttatctgca atttttaagg
     3001 aggaaaaaag accaatcgtt aatattaaaa actctgaaat atctgtttta tcacctacaa
     3061 gagacaagta tttgctcaaa tttattttcg tttcctgata gctgggatca ccaatcgatg
     3121 gacgaaatgc aggtaatact gaatcaaatg agatatcagt taattgtgac atgattgatt
     3181 ttgcaataga ggtatccact tttgcaatta aaagaggcag taactctttt ttggttttca
     3241 tgcttaaagt ccctgtcttg ctatcaagag acaactcagg gatagactgg aaagcattca
     3301 tcagaatagt gagcaatact ttctcacttt cattggcaag catctcttgc acaaactcca
     3361 gaagctcagg tgagagcgga tgtgctttgt cctgactaaa agcaccgagc atctccaggt
     3421 cacttgagta aggtattaat tcaatttgtc gtataatttc ttcaagtagt ggtttattat
     3481 gagtcaatag cccttttaag aagagagatt ttaacaatat acgcttggat ttcggtgttt
     3541 cctcaattgc ttgccaaatc tgcttaaatc gagaaaaacg tatatttttc ataaaatcat
     3601 ctaaaatttt ttgggtgtca atagtgctga ctgtagtatc tacggagaat tttttggtta
     3661 cttcgttaat tttttgaacc gtttcatcgg ataccctaac cacatcttca gcaactttcc
     3721 ttgataaaac acttagctta ttagccgttt ccttcgaagt ctttttcaga tcttccccca
     3781 gtttctttag aatctcatct aagttttgca tataaactct acctcttaac attgtcttgc
     3841 ctgacaactt atatttcaca gggggtatga taaagcacaa agattaagaa aatattaaac
     3901 ttattgttca aaattaaaaa aaagtgtcgt ttatttaaat taccacctga attgatctaa
     3961 ataattatta ataagtaaaa taaatgaaca aaaatattgt catttataca gacttcatgc
     4021 taagattcag ccagtttgat ttttagaata ctatggtgat agtttaaaat aggtgattac
     4081 aatgggtaga agtgaaatga aaatagcatc agctgaattg cgcgaattga tgaaggcagt
     4141 atctgaaggt cattatgaaa ctgtcaatac aatactggat aaaaatcctg agcttgtaaa
     4201 tcagtacgtt ccccccactt acgattcccc cctggcaaga gtattaaata aaaaacacat
     4261 tgactacaaa atgcttgata ttttggttaa gcaccatgtt gattttgatt atcccatcaa
     4321 ccatcacaaa gaaacaccaa ttgaattggc ttgtaaaaat caggatcttc aattattcaa
     4381 atacttagtg caacacaacg cccccatttc agagcatgca ccacatttcc tgctggtgaa
     4441 tagcaccaat atcaaatacc tgacagaaga taaaattaaa aatacctgtg aaattattaa
     4501 attaatgggt ggtttggaag ccgtctcttc taaatgtgat gcggaaggta atcgcttcga
     4561 ggaactggca aggaaatcac aattaatcaa tcgtttcggg ggtatagtaa ggtacgatta
     4621 cgtgcaattg ctgcgatcga tttatccaat tgttgataga gaagtggatg ttccaaccat
     4681 taatgcctct actgaaaaac ttacagacct gctcaataaa attagggatc agcacacctc
     4741 aaaagaagga tacgatcaac aaaatttaaa agattctatc agtcttttct ttatgaccgg
     4801 cggcgaagta cctccttcaa gaaaagttcc agagagtcgt tttgaagaag ctggcataga
     4861 cgcccctaaa aacgcgcttt aaatttattt atcctatcta ttaagtcgtc aagaagcacc
     4921 aaatttacta tttggcgctt cttggcacct cgcatctctc ctttatataa ctctcaactc
     4981 ataagaactt gattgtgcct tccaagccaa tacaaacgat tttatgatta cccatttatg
     5041 caattttttt ggctacaata aaaaatttat gccaatgctt ataataaccc aaggcatcca
     5101 tttcatcctt ttcttcttct tgaaaatatt caattttgaa ttgggaaaac aaatggcaaa
     5161 tcatttcccg atttaaaaaa atcatatccg ttctggtatt ccagaagtca ttaaaaccaa
     5221 aaaacgaacc agaaaatcga ccacctggct taagcccttt taaaataacg ttccagaatt
     5281 tataaaagta aactggcttg agaaatggaa gactaaagct ggcgttaatc aagtttactt
     5341 tagggagtga atccaaagcc tcaaatgaag aaactattgt ttttagtttg ttcgtgcatg
     5401 ttaattcttg agctgaagaa agcaaactgt ctatagcaga taattgtgaa tcaatggcaa
     5461 tcacagacca accgcatcgc aaaagttcca taacatcgat accggcacca catcccaaat
     5521 cgattgccga tttagataga ttgatctttt ccttatcaaa atttgcaatt gctttaagca
     5581 aagactttct ggggagagtg ttttgtttcg tagaattata ataagccgtc caatttcttt
     5641 tattcattta ctatatcccc tctagttacc aaaaggtaac taattgactg attttattaa
     5701 atattttagt ctcgaacctg ttcattttat aaaataaagg agtggattat gaaagaaaat
     5761 ctcattaaaa aaattgtcca gaaaaaactg ctaaaaagga atagagggtg gtgtggtcca
     5821 ggaacttgta ttggatgata ttactgcaat gatggcacgt tatcaaaaaa cgtgccgtta
     5881 cacttaaact aatcaaagcc ccataatcat gcatcctgac ttaaaagact tacttgatta
     5941 cagaaatcat aaaattatta ccaggtataa tatggattac ccgactgcag ccatgcaagc
     6001 tgaagaggca ttacatgaat taatgaaatt tatttggctc tgccataaac ataaaacaga
     6061 taaattactc tatcctgatg ataaaaatct ggatttctct tgtgttattc atgctgaaat
     6121 ggctgatatt gacaagatgt ggcatacttt tcttttattc accagagatt atcatgagtt
     6181 ttgtgatacc tgtctcaatg gagttttttt tcatcatgac cctttaattg gggaaaatac
     6241 ccctgtttct gaagaggatt atgctctgga gttgacccgc tatttaacct atatccatga
     6301 ccatttgggt tcggaaactt taatcaaatg gtttcaataa atcaagcatg acaaccaagc
     6361 ttctgttcgc aaaatgtttt ttccgcattg attattttat ccatattggt ttcagtccaa
     6421 cgagtaagta aaaccaatat ggcacttaat tccttaccca atctggttaa tgaatactca
     6481 accttcatag gtaaaacagg atacgattgt ctttggacaa agccatatcg ctccaatttc
     6541 agtaatgttt gcgtcaacat tttaggtgat attccaccga tttcacgttt taactctcca
     6601 aaacggtata ttttttggga taaaaggcta atgaccaata tagaccattt atcacttata
     6661 ccttcaagta cattatgtga tgggcacttc tcgttataaa cactataatc aggatgagac
     6721 atatttgtac tccctatttc tcattaataa cgaccttcaa actgaataaa atgggttcgc
     6781 gctttattca tcagtaactt aaatcgttgc aaatgctgtt cagatgccgt tgaggaatgt
     6841 aaaacaaact ctgaaataca taatgcaaaa accaagcgcc gtatttccca aatcgtttct
     6901 tgaaaaatat caattctttt tttagtatgt tcacttgttt taaaatccag tttcaaattt
     6961 tcggagctga tggatatcgg gagataatgc tcggtgaatt ggccacgtaa tttctctaca
     7021 aagagcaatg tattaatttt ttctgtattg gattcgtcta tttttcgcac aatatcattt
     7081 aaaaaatcat aaaccatcac ctgatgaaaa aaataaagtc tatgagaata acggtatgcc
     7141 tgaaataaaa aataatcgcc tgccaaaaca ataaaaacac ccaacagtgt acatatgcct
     7201 cgattaagaa ccatctcaaa aggaccacga ggcgatgtcg cctcttccgt ttgcgccatc
     7261 aaaaaaaaga ccactaaagt aataaaaaat acactaatgt cgtaacgtct tgtgtttaag
     7321 gtagcaacac tcaaaccaac tatcgccaga acaaataaga ttgatatcaa tcgatagttc
     7381 aattgcaata aatagagtaa gggaatcaaa atgatgagag ccaacagtgt ccctcctatc
     7441 ctgtgtttag ctcttctgat aatcaatcct ggttcaatac ccgcacttac tgacaagaca
     7501 gtgatcaata cccaccactt ttcgggaaaa gtcgaaaaca aaaaaatgag catggcaacc
     7561 atgaacatga tgactagatg aataaatcga gcataatcaa gttttttctt caatgctgaa
     7621 gtatcatgca taactgattc cattggctaa atgcttgttg caaacatctc ataacatatt
     7681 gttgtaattt tgactcaggc atgatggaca ttttaggagt gccacagaat gtgttggttt
     7741 taatcgcaca tctaagccta taaagattct ctttcactcc atgccagaaa gcctcatttt
     7801 tggcttcata atagagatta tccaaagaaa attgaatgtt gcgtatgttg attgaaattc
     7861 gctgagtata aatcatgtat tttttgggga taagtctacg atattgccta agcataccca
     7921 aatgagtcat ttcttcctgc atgaatttgg gaccacggtg agtaaaagaa ccttcaatat
     7981 ccagctcaag ggattgaata aattttgcca aagccctttt ccatacactg agatattgat
     8041 tggggaagaa tcttaaacaa atgaatatgg cgatcataga taaagcgctt gacccaacga
     8101 tgtcgtagac aatttgccaa tttgcaggag gttctgtact taaaattgtc gctccagaag
     8161 caataatgag catagtcaca ttcctcaatg gaagataatt ttttagcaca taaaaataaa
     8221 cgcttgctaa cgcaataatt gagaaaaaga aaaatacaac ccgaaatggg taaacaagat
     8281 aaaatgaggt actcagcaat accactacca ctccaataaa aatcaaaaag tgttctttct
     8341 ctttaaagct ggtaagactg ggtgattcat aaaatcttac gagcaaaaat ggtgaaaaaa
     8401 atgccgtaaa attcgtggga cgaaataccc aataaacata ggccattacg gtagcaataa
     8461 aaagtgattt atagagcgct attcgttgaa cagcataagg atcaacctca tcaatccatt
     8521 caataaactt cgacatagac ataagcgcta gctccaacac gaagcgggta tttagggtca
     8581 gggtcagtaa cctttataat cacaggaagg cgctggggca ataaaatcca ttgattttca
     8641 ttgataacat tttgcagctg agtacgatca tccaccaatt gcctattcgc ggaccaataa
     8701 tccgaatcaa taacaccatg aaatattttt cttcctaaat acattcttgg aaatatcaat
     8761 acctttgagc ccttgcgcac atcacgcaaa tccgtctcgt taaaattggc ctgaatataa
     8821 atattatcct gatcaaccaa tgagaataat ggctgattga tattgactgg agttcctatg
     8881 gtaaaaaaca aattttgaat aactccatta ccctgagcat aaacatccgt taaatccaaa
     8941 ttgactttgg cattttttaa ccgggcctga atggatttta tttcattctc ctgaactgtt
     9001 atttgatgct gatcaatttc aagttgtttt aatgaagcct gccatttatc tttagctgct
     9061 tttgtttctt gctgagagtt ttgcagggta atcagagaaa cagattttat agcgtaccct
     9121 ttaaggtatt tttgatcatc ttgcgccagc tttttgtata ttttttgctc attttcgcta
     9181 agcttcaaat cccgttcgta cgtagctttt aatactgcca actttgcctg tgctccagct
     9241 aaatcagcac tgagctgttc aactgtgtat tcatagggct ttttgaatac ggtaaatagt
     9301 ttttgtccct tacgcactgc atcaccattt tttacataaa gatcggttat atatccgctt
     9361 gttaatgccg caaccggacg tacattgttt actacaaacg cattatcagt aaaagggatc
     9421 aaataggaaa aaacatagat aattgcagta agaaaaccga taaaaataat gacgttagta
     9481 agagtaaacc agcgttttgg ttttaaacga agcaagtact cttttatttt tccaccatat
     9541 tccatcaatt ttttcataaa atagccaacc agtttccctt ggtatataac aataatgcga
     9601 aattaactta tctcatcgaa cagctcaaga gaggagaact gcaattttag cccgcctggc
     9661 gacttttaaa atcaatgtgt tacaaatcaa actgatttag tcactacagg catacccacc
     9721 agccaaatct tgatacagca taaccagtga catggccaat tccagcttag cctgatttgt
     9781 tgagagcgcc agggtatcca gataaacctt actctccaat aactctttat aggacattaa
     9841 ccctgatttt aataaaccat tttgtaattt gtatttatgc tgataatctt cttgtgcgtg
     9901 ccgataagat aaaaattgct ctctcatgcg tttattagct gaaaaagcat tatcaacttc
     9961 ttttaaaatt aatcggactg tcttaacgta ttcggccaat tttgcattat aaactccctt
    10021 gctaactgaa attttaccca gagtatttgg attaattgtc cagtttacgt acgcatcggt
    10081 cgcttgttca aacgtgctat caggcaaatg tgcctctcca ataaaatcat ccagctgcat
    10141 aacaggaaaa aaatcactat aagcaacaaa aatacctgca cgagctgctt ttagggaata
    10201 ctccgccatc ttcatatcag ggcgattacc taatacagta gcgggtaaac ttccgggttt
    10261 aaagcgggta aaatccaatt gagcaaaatt atttttgctt ttgattaaac caggattctc
    10321 atttattaaa taacgcaacg cattctgact aacgacaatg ttgtgtttaa tgggttttat
    10381 ttgtgctgca atcaagcgct cgttggattg caactgagcc aaatcaatat cattttttaa
    10441 tcctatgttg atttctttcc tgctcaaagc aattaacgac tttaaatccc tatccagttc
    10501 atacaataat ttaagttgct ccaactgtgc aatcaaagtg aaataagccg ctgccacctg
    10561 accaatcaag gtcaaacgca aaccatcgat tgctgcctga tgatattgga cctgatattg
    10621 agcacgcttt tgctgagtat aaagttgcat aatattcaaa atataacttg gccatattcc
    10681 ataaaaccct cctggcacac caagagcagg attagttgaa taccctccca atatttgaac
    10741 attaggtatc caactcaact tcacttgctg taactctcct tgagcctgct gtaaattagc
    10801 cattgcaata tgaatatcca tattggtctt caatccagta tcaattaaac gatccaattc
    10861 tggatcctga aattgcttcc accaggccaa ataaggcaat tcctgaactg gtcgataatc
    10921 cctgggactg gaggggaatt gcttcggcgt tgtaatattg gaaatttgct gatcagtgct
    10981 ttgaccacag cttaacaaac ctgctcccaa caataataag aacattaatt ttttaagcat
    11041 gcacatcatc gtttatttca aagtgaatgt ttatcattgt aatttataat tcatctaatg
    11101 gtgaaagtat atttaaattt caacaaggag ctctcatgat caaaaaaata gcatttacca
    11161 tgtatccagt tcaaaatatg gatagagcaa ggcaatttta tgaaaaaaca attggactta
    11221 aagtcggtaa aatttccgct gagggagcat gggtagaata tgatttacct caagggggtt
    11281 gttttgcaat tactacattg gctcaaggag ttatccccag cgctaattcc ggaggtagcg
    11341 ttgcttttga agtagataat cttgatgatc tggtattcca actaaaatcc aaaggagttc
    11401 aattcaaaat tgacctattc gcttcacctg tttgcagaat ggcgattgct gtggattcag
    11461 agggaaatgc gttcactttg catcaattaa catgattgtt gctgccaata cgtgtgaaaa
    11521 tctctaaaga gagcctcaca cgtattggtg ttatgagaaa catcaatatt tccaaaaata
    11581 caaaataaca atgttcctta gtatgccttg tttactaaat cacccaaggt gggcagcaga
    11641 ctctttaatt cactttcagg gtaaggaata gaaggtatgg aagattgcca aataccttga
    11701 ggccacaaag gatcattttt gctgcgaata acaatatgta tatgcaattg cggaacaata
    11761 ttgccaagag cgcctatatt aagcttgtct ggtttaaaat aatctctcac cagaagagac
    11821 agttgattga tttcctcaat taataaaaat cgctcgctct tttcaagctg ataaatttct
    11881 tgaatatcca tttttcttgg aactaaaata aaccaggggt atgaggcgtc attttttaaa
    11941 agaaccgtag ataaaggcca ctctccaagt ataaaagaag acgacttaat ccgatcatca
    12001 atgcaaaaca aaatactctc tctatcaata agtactcaat cattataaca aaaacaagac
    12061 cttcagatta taagaattac agataaattt taataaacct attgagtaat gatccctttt
    12121 tgcgttatgt tgaaattacc tattgtaaaa taggctatta acaattgtca tttaaggaag
    12181 aatgcaattg ctcagctgac cgcttatgtc agtcgacgat tgataagctt tagcataagg
    12241 aggattcatg gctagaggcg aagtcaagtg gtttaataac gccaagggtt ggggttttat
    12301 tatcccggaa ggtggtggtg aagatatttt tgttcacttc tcctcaattc acggcacggg
    12361 atacaaaacg ctggttccag gacaggcggt cacctatgat gtcgttaatg gtgaaagagg
    12421 tttacatgcc tctaatgtcg ttgctcttag tgaaaacgcc gaacaagaag aaatagctta
    12481 acccaattaa tgtgataaac tattaagcgc ggcagtataa ctaatattta tgctgctgcg
    12541 tccatcaact tgaggtatta tgtccttcat tttataactg gactttagtt gatgagacgt
    12601 ggtttattac tcttaaatct tggcactccc gataatgcgg atatcagggc ggtaaagtta
    12661 tatttgcgtg aatttcttac tgacaaaaga gtaatagact taccaactat cccgcgctat
    12721 atcctggtat attgtttgat cttacccttt agatcgccaa aatcagccca ggcttaccaa
    12781 tcaatatgga ctgaaaaagg atctccactt ttgtatcata gccaaaatct agtgacaaaa
    12841 ttacagtcct cacttaagga tgagtacaaa attgctttgg gaatgcgcta tggaactcca
    12901 tcaattacaa cagcacttgc agaattaaaa gattgccatt cccttaccat tttaccctta
    12961 tttcctcaat attcttctgc tgctacaggt tcagccattg aaaaaacctt atcctatttg
    13021 gcaaaccaag aaattattcc atcaattaaa atcattcgag atttctacca aagaccagaa
    13081 tacatccagg cgcaagctaa aattatgaag ccttatatta aagacaattt ccacgttctt
    13141 tttagctacc acggaattcc cgaacggcat attcacaaaa gcgggtgcga tacgctttgc
    13201 ccccaaacat gcacacccat atacgataaa aatcaagcat gttatagggc tcaatgctat
    13261 cagaccagtc tattactggc aaaagaattg caattaggca cccaccaata cacaaccgcc
    13321 ttccaatcca gactcgggaa aactccatgg ataaaacctt ataccgatga aatttttgcc
    13381 gaactaataa gcaaaggaat taaaaacatt gtggtctcct gcccatcttt tgtcgcagat
    13441 tgcctggaaa ctcttgaaga aataggaatt cgcgcaaaag aacagtggga aaaacttggc
    13501 ggtgagcaat ttatattaac tccttgcatg aacgatcatc ctgagtggat caaagcaatt
    13561 cacagtattg ttaacgagca aatttaacct gccgtcttgc tgatttaacc atcggcagtc
    13621 gcaacaaaat atcgttttac attaaatatt tattgcaaaa caataaatat ttaataaaat
    13681 acgcatgatc tcatcttaga aggacaatta tataatgaaa aaaataaaat ctgtgagtca
    13741 tttgcttttt ctattattca aaacattagc ctatggcctt ccccttgtaa cgacttattt
    13801 cattctattt catttcgaga gcatgctgaa ttggggagga tggcactccc tgttatcaac
    13861 cccagtgaaa gacccctctc atttttcctt gttgcatcgt ttcattattt tattgattga
    13921 agcattaccc ttatccatta atgttctaat ttgtcatcaa ttggcaaagc tgtttcgctt
    13981 gtatgaacat ggctatttat ttgaagaaga aaacattcga ctgattaaga atatcagtat
    14041 ttacctgatt tcaggtgaac ttatacaatt gctttatcaa cctttgataa ccgcagctct
    14101 gacttttacc aatccatctg gtcaacgtat cgcaagcatc accttgggaa cagcgaacct
    14161 gtccacgtta attaccgctt ttatcatttt gattgcctct tggattatta aggaagcaca
    14221 taaattaaaa acggatgcct tgttaaccat ttaatgtgaa cttcagcgta aggacatcaa
    14281 gagattttct tacgcagtct caatcagttt gtatcccgct tgtttaagga gtttcaatcg
    14341 atgtcaatta ttgttaattt ggacgtgatg atggcaaaaa gaaagtgtcg acttagagat
    14401 ttagctgaag ctataggcat tactgaagct aacttatcca ttttaaaaaa tggtaaagcc
    14461 aaagcaatac gttttgcaac ccttgaggct atttgtgctt acttgcattg tcaacccggt
    14521 gatcttcttg agtatcaatc cactgaagac aatcacctta tcaaagacag ggcttaatct
    14581 gagaaagctt tgagtaagga cttgcaaaaa accagggtca aggcaaaaag taattgatgt
    14641 taaacacatt ttacaataat ccgccctaaa gactatggtc tgaaaaactc atttcctgta
    14701 ctatggcgac cttctttttt aaaactaact actatcagga gattatgttg aaaaggatct
    14761 ttttcttaat gatttttttt gtctctcaaa ctatggcttc agtatttacc catgaagagg
    14821 ttcaaatatt aaaaaaacat tttttaaata actttcaaac caatggagcc atcgtagcct
    14881 caccctcgca gtacaacccc aattattatt atgactggat tcgagactca gccattgcca
    14941 tgggattggt tgagacatgg tatgaagcca gccaatctgc tcgatataaa aaacttttat
    15001 tagaatacgt ttcatggaca gaaaaaatac agcatcaagc ggatcccatt gctggccagg
    15061 atatattggg tgaacccaaa ttttatatca atggcaatcc ttttgatggg gaatggggac
    15121 gaccacaaaa tgatggtccg gcactcagag catctgtttt aattcgtttt gcgcaacagc
    15181 ttctcgatca taatgaaata gattatgtca aatcacatct ttataacaac accatggacc
    15241 cacaatccat gggcactatc aagatggatt tggaatacat cgcacatcat tggcaagatg
    15301 caaattttga cctgtgggaa gaggtttatg gccatcattt tttcacggct atgacacaac
    15361 aaaaagcgct gaccgatggc gccattttgg ctcaccagct tcatgacaga caagctgctg
    15421 ttttttatga aatgcaagcc aacttaatta acagtcgact gcaacagcat ttggaccatc
    15481 agaataaaat aattcaagct acattattac ctcaccctgg tcctcaaaaa acactagagc
    15541 ttgattcgtc cgtcatgttg ggtattttaa ctaatccaca aaaagaaggt gtttttgccc
    15601 cacatcacac ctttgtgcga aatactgcaa aggctttgca tgagcaattt aatttgatgt
    15661 ttcctattaa taaaaaccgt tctggagcaa tattgtttgg tcgttatcct ggcgatactt
    15721 atgatggcta tcaaacaaac agcataggaa acccctggtt tatcttaaca gctacgatgg
    15781 ctgaatatta ttttaccatg gctcataatc tgtccctgaa cagcagtaat aaacttcata
    15841 tacagaacta tctaaagaaa ggggacaatt acctaaggct gataaaacaa tatggccctg
    15901 atctgaattt aagtgagcaa attaatttga ataccggagt tcagcaagga gcgacatcct
    15961 tgacctggag ttacgtttcc gtcttgcgag ctattcattt aagagagcaa ttggaaaata
    16021 gaataaaaac tatgggctgg aatacctatt aggattttat acgatcaggc aatgcgataa
    16081 agaatagaat ttgtggcccg tgtcagttaa agataacacg ggcatcagag gaaaatttgt
    16141 atcaatttgt tgaagccagc aaagaagatt ccgatttagt tgacacagca ttaacctctt
    16201 catcctctct acctaaaaac cgtaatggct tgccactcat tatataagtt tctatctgct
    16261 ctaaactaat attcctggtt tcaggcatat aaatataagc ataaatgaac cctaggaaac
    16321 agattaccgc atacagaacg aaagtcactt ctataccaaa catttgatgc agaacaggaa
    16381 aactaaaaat aactatagta ttaaatgacc aattactcat ggatgacatc cccataccgg
    16441 caccacggac atgaagagga aaaatttctg ccatagcaat gtgcgggata gggcccacgc
    16501 tgattgcaaa agagaaaata taaaccatta agcaaataac ggataaataa gatagccagg
    16561 caacatgatt taatgaaaac aacgataatg caaacaaact caaactggtt cctgcaaaac
    16621 ccagtaacag taatttgcgt cgccctagct tatcgacata caaaatggca ataatagtaa
    16681 ccagtaaatt aactaaccca atccccattg ttgctaatat ttgtccagtc gtgctgccca
    16741 agcctaaatt cttgaatatt tctggtgcaa aataaattac cacattgata ccgcttaatt
    16801 gttgtaaaca aaacaatatc gttcctaaca tcaacacagg taacaagggt ttttggaata
    16861 gaagtagcca gttgccttgt tttggttcgt tcgccagtgt ggcttcaata tcttttaact
    16921 cttgctcaac tggttgtttg cctctcaatt ttcttaagga gtttgctgct gcatcatgac
    16981 gtccaacgct gcataaccac ctgggagact ccggcataaa caaaatcccc agggtcaata
    17041 acaaggcagg gatagcactt gaagcaaaca ttgcacgcca cgcttgctgc tctatcaata
    17101 aataattgac cgaataagaa caaacaatac ccacagtcaa tgcaagttga taaattgcaa
    17161 ccactgcacc acgtgatcgc atagcggctg tttcagccag gtataatgga gttaaaactg
    17221 aagccattcc aatggccaaa cctaaaatta accgagataa aatcagcatt gaaatggttt
    17281 cagcaaaacc ggcacccagg gctccaacaa agaacaaaaa accggcaaag gataataaag
    17341 aacgtctgcc gaaacgtttt acgctctttg atgccatgaa tgcaccaatc aacagcgaac
    17401 caaacaatgc cccaaaaggt aaagcagaag ccataacacc aatgtgggtt gcattcagat
    17461 taaaatgatt ttttaccagt ccaagagaac ctgctattat tccctcatcg taaccaaata
    17521 aaaaaccggc aattgaccca attattgcga caatccaagc catttttcac tccttgattt
    17581 taaattcttt tcctctgtct ctactctaca caatgaactg ttttagctgg cactatccct
    17641 tacatcacgt gaaaacattt tttcaactcg aatagattaa ttctgcgagc aaagttctcg
    17701 cttctgagaa ctcaccgcca tacataactc tgtaattaaa gaccagttat gattcttgat
    17761 agcctcttcc ggtacaatcc aggaccctcc aacgcaagct acatttggca agcaaagata
    17821 ctcctcataa tttttggaat taattccgcc tgttggacaa aatttaactt gagggaatac
    17881 ccccgatatc gcttgcagca tagggatacc tccagccgct gccgcgggaa aaaatttaaa
    17941 atggttatat cccatcccca agccctccat taattcagaa acactggcta cacctggaat
    18001 gagaggaatt tcacttttct gtcctgctat taataattcc cttgtttggc cagggctaat
    18061 ggcaaatcga gcgcctgctg ctgccacatc atgaagttga cccggggtaa tcacagttcc
    18121 ggcaccgatt aattcatctg gaaatgtatt gatcaataat tccagtgctt ttattgccac
    18181 tggtgttctt aatgtcactt caagaacatg aatccccccg gcaaataaag cctcggccaa
    18241 aggtaacaca tcccctaact ctttgattac aatgactgga ataaccggag atgcagcaaa
    18301 tacagcgcct ggttgtgttt tccattcaga aaaggacatg agaagctcct gtcaatattt
    18361 gttttaaact gacgctcgcg cctaaaatac ctggttgagc ggctgcaatg acataagtgg
    18421 gaattaaggc attaaaatca ctaaatcgtc ctttgtcttc aaattggatt ctaaagtcgc
    18481 tttggtgtac caaggagagc aaacgcggca taatccctcc tgcaatataa acaccaccaa
    18541 aagctccata agttaatgct aaatcaccag caaacgatcc caaacttgaa aaaaattggg
    18601 ctactgcctt atgagctatg aaacattcct gatttaatgc caaagatata atttgcgctg
    18661 ctgacaaaaa ttcaactttt ttactatgat acgcggctaa agcttgatat aaattttcca
    18721 gtccttgacc agacagcaaa cgctcataag atacatggga gtatttactt tttaaataac
    18781 gatagataaa ccattcctgc tcagtttttg ctccccaatc agcatgcccc ccttcccctg
    18841 caaaagcact atagtgatgt tgatgaggaa ttaaataagc aacacccaaa ccggttcctg
    18901 cgcctaacac cactctcact ttactggcat ccaggtaacc attaccaatc tgcaccagat
    18961 cctgagtcga taatacaggt aaactcatcg caatcgcagt aaaatcattc atgacttcca
    19021 gaacttcaag gccaagtctc tgttttaact ctgttattga aaattgccaa tgacaattcg
    19081 tcatagaaac caaatcatcg atgactggac acgcaatcgc tatcgcaata tgttttattt
    19141 cttgcaaaga atgccgggtt ttataagtta atagcgcgga ctccaaacta ataaactcgg
    19201 cacaaggaga aatttcaatt ttatccatct gcaaattgac aagattaact ctactgaaac
    19261 gagcaaaagt accgcctatg tccgcaacaa tcgcatactg cgctaaatca tgaggcatca
    19321 taaaaaaatt catttccttt aaatagacta catgccccct gttctgcacc agtaaaatgt
    19381 aatcttaaat taccaaacaa ctctcgtccc atgcctagaa cagaggaata cccttcgata
    19441 gcaactggag agcggcctgc taactcttca tcagaaacta ataattgaag tattcctctt
    19501 tcacaatcga tcaaaatcat atcgcccgtt tcaattttag caatcccgcc cccatcaacc
    19561 gcttccggag taacgtgaat tgcagcggga accttgcccg atgcgccaga cattcgacca
    19621 tcagtaacca atgccacatg aaatccttta tccatcaaca caccaagttt tggcgttaat
    19681 tgatgtaatt ctggcattcc acatgctttt gggccttgaa aacgaactac caccacacaa
    19741 tttttattaa gcaaaccttg attgaataga tgttcaaatt cctcttggct tgagcaaacg
    19801 actgcgggag cttctatcac ataatgctca tcataaagac tggacgtctt aataattgct
    19861 ctgccaagat tgccacttaa aacctgcaaa ccaccctgag gtttaaatgg atcggcagca
    19921 gaggttaata cgtcattatt gcttgagctg tcaggaccat taacccaaac cactcgattg
    19981 tcaatcaatt gcggctgctg cgtatattta tccagtccaa aacccacgac tgtgttgaca
    20041 tcaggatgaa gtaactcgcc ctctagcaag gttttaacaa aataagccat gcctccagct
    20101 ctttgaaaat gattgatgtc tgcttggcca ttaggataaa ttcgagcaat taatggaata
    20161 attgctgaca attgtgcaaa atcatcccaa tttacccaat accctgccat agccgctatc
    20221 gcaacaaggt gcatggtatg attggtcgag ccccctgaag ccaataatcc gataataccg
    20281 tttacaatat ttttcacatc aagcatctgc cctattggca tataatcaga atttaaatcg
    20341 gttaatgaca aaatctgaca tgctgctgct ttggttaatt cattgcgcaa cggggtatta
    20401 ggattcacga aggaagagcc agggagctgc aaccccatca gctcaataac taattgattc
    20461 gaattggcgg taccataaaa tgtgcatgtt ccagccgtat gataggaggc cgcttccaca
    20521 tccaataacg catttttgtc gacttgacct gctgcgtaca attgacgaat tcttgctttt
    20581 tcctgattgg agataccaga gggcattggg cctgcgggta taaaaataaa aggcaaatgg
    20641 ccaaatgcca gtgcagccat taataatcca gggactattt tatcgcaaat acctaagaga
    20701 agccctccat caaacatgtt atgtgacaaa gcaacagcag tcgataaagc gatcacatcc
    20761 cgactgagta agcttaactc catccccggt tgtccctgag taatcccatc gcacatggcg
    20821 gggacccccc cggcaaattg cgcaacgcct cctgcagcag agatcgcttc cttaatcaaa
    20881 tcagggaaag tagagtacgg ttgatgcgct gataacatat cattataagc agaaacaata
    20941 gcaatattgg cttttgcatt acctctcaaa atagccttgt cttgtttagc acaagcagca
    21001 aaaccatgtg ccaaatttcc acaatgcaga atacttcgtt gtgggccttt agtacgagcc
    21061 ttatcgattt gattcagata agctgctctt ctagcagcgc tacgattttg gatattttct
    21121 gttacttgat ttattacagg atgcattctt tttcctcatt gtttggggca tacataacct
    21181 gaacattggc atccggggca tttaaaatag cgcagattgg caggattttg gggttattag
    21241 ttgccattgc ctgctccaga acaacctgtt ttctttgacc tactaaatgt aaaaaaatag
    21301 tactgctgtt taataatcgc cttttcgata ggctaaccct ctggaatgaa gctgttctag
    21361 gcttcacaat aagcaccgca tttgctttat catctaatcc tgcacttaac tcatcactgc
    21421 atggaaataa agaagcagta tggccgtctt cacccattcc taaaactact gcatcaaatg
    21481 ttggtagaga atcaatttgg ctattcacct cattcaaatg acctaatgga tcctcttttt
    21541 catcaaacaa gctgataaat ttggcttttt ttgccttctc ttgcagcaga aattctcgca
    21601 ccagcagctc gttacgatgc ggatcaaaaa ggttgacaca acgttcatcg gtcaacgtga
    21661 tcactacttt ttcccaaggc aagtcggcta aagccaattc cttgaacaaa tcgatagggg
    21721 ttctaccgcc ggaaaccacc aaaaaagctt gccctcttcg tttgattgca tctgatagaa
    21781 tagttcttaa tttttccacc atgtcatgaa tcaactgttt ggcatcccta aaactatgaa
    21841 actgcattgt gatcacccca aacatgtgct tgcccgttaa ataagcgtat gacttccttt
    21901 ggcccccagg tacaagctgg ataggtatac agcggaatat tatcttcttc ccatgccgat
    21961 acaatactgt caatccattg ccaagcctgt tcaatttctt ctcggcttac aaaaagatac
    22021 tgattaccta acatgacctc tagcaataat ctctcatagg catcagcaat cctttgcgtt
    22081 ttaaaactat gatgaaaatt taaatccaac ttggaatcat gcaacatcat acgctcacca
    22141 agacccggaa ttttattcat tatcctcacc tccacccctt catcgggttg taagcgaata
    22201 attaactgat tagggtacaa ttcttcattt aagggatgaa agatattata aggctgtggt
    22261 ttaaaataaa taaccacttc gctttgcttt ttgggtaatc ttttgccagt aagcatatag
    22321 aatggaacac cagaccatcg ccaattatca acataggctt tgatagcaac aaaagtttct
    22381 gttgtagatg cctgattagc acccacctct tccaaataac caggtatttt tttaccattt
    22441 gctaaattat tagccacata ttgagctcgt actgtatgat catgagcatt ggacttgtta
    22501 ataaaacgca aagatttcag tacttttaat ttttcgcttc tgatactttc tgctgaaagg
    22561 cttaaggggg gttccatcgc aactaaagat aatatttgta acaaatgatt ttgcagcatg
    22621 tccctgactt ggcccgactt atcataatat tcccaacggc cttcaacacc gatttcttcg
    22681 gcaacagaaa tttcaactcg atcaataacg cgattatccc aattggttga aaatatggaa
    22741 ttagcaaagc gaagcactaa taaattcaag acggtttctt tacccagata atgatcaatt
    22801 cgataaatct gatcttcaga aaaaaaacaa gccacttgtt cgttggttgc tacagaagaa
    22861 ggcaaatcat ggccaatagg tttttccaaa acaactcgcg atggtttctg tgttagatta
    22921 aggcgagcaa gactgcggca agtttgagaa taaaggcttg gaggaattgc caagtaagaa
    22981 ataaccactc gctcagaagg gttaacatag cccgttaatt tttgataatc atctgattga
    23041 ttcagatcaa gcttgcaata agccagtttc tttataaaac gtaaccagac tgcttcatca
    23101 atttcatcaa ataaaaattg ctgaatttta tttttaatca agtcagcata agcagaaaaa
    23161 tcgagctctt ctcgggcaca cccaataatt cttgtttttt cgtgaagtaa attagccagt
    23221 tctagttgat ataatgcggg caacaatttt cgacaagaca agtcacccag cgtaccaaac
    23281 aaaattaaat cacaaggata cttcgattct gcttttaaga gcatatatca tcccaaaatt
    23341 acataactat gtccctgaaa ttgcatcgat tttacatcac tggaatcaag cgtcaatcaa
    23401 aattgatttt tttcgctcat tctatcaggt acatcacctg tttaatcatt caagtcaatt
    23461 gaagtgaatt aaaagcggta aagataaaaa aggattaaaa aatccacttg gaaatccgct
    23521 ttatgaattg caacattatc aaaattagcg atttttgtct aaaaattgca aaacttcatt
    23581 tttatgctat atgaaataag tacacgttag taaaaaatcc ctttgatcag gagccactca
    23641 tgcttaacaa gagattttca gaacgactga ataaagaatt ggataatatt ggagttcctg
    23701 aggccacagc agagcgaatt gagatacttt ccaaacttat caaaattcca aaatttaaag
    23761 cagaagcttt gcttaatgga attacaagcc ctgatgaagc cgtattaaaa acattagctc
    23821 aggaactgga agtcaatgcg gattggctaa ttggcaaaag tgactccaaa tcaggagccc
    23881 attagaaatt atccatgaaa tctatgattg gaactaattg gcttcgcttt tttctcaatg
    23941 tattcaataa tttttcctgc caaattaata ttagttgctt tttctatacc ttcaagacct
    24001 ggagaagagt taatttctag caccaatgga ccatgatttg agcgaatcag atccactccg
    24061 gcaactctta atcccattgt ttttgcggca ctaacagcga ttgcccgttc ttgtggagag
    24121 agtttgactt ttactgcttt tcctccttga tggacatttg cgcgaaactc cccatccttg
    24181 gcctgccgtt tcactgcggc aactactttt tctcctatga caaaacaacg tatatccgta
    24241 cctcgggatt cttcaatgaa ttcctggacc aaaatattgg catgcatttc tttaaaagca
    24301 ttgatgatac tcacagctga ttgatgactg tcagcaagaa tgactccttt gccttgtgtt
    24361 ccttctaata attttatgac cagaggagcc ccaccaacca tatgaatcaa atcttcagta
    24421 tcatcaggag actgggcaaa actggttaac ggcataggaa ttcctttacg tgccagcaat
    24481 tgtaaagagc gaaatttatc cctggatcgt gatatagcta tggattcatt aagagtgtac
    24541 atgcccattg tttccatatg acgcaatact gccgtaccat aataggtaat ggatgcgcca
    24601 attctaggaa taacggcatc aaaatgcggt aaaggcgccc caccccgata atgtacctta
    24661 ggattagaag cagccacgtt catataacaa tataaagggt taataatttt aacctcatgc
    24721 cccgcagcct cagcctctgc ctttaaacgc ttatgagaat acaaatgggg gttagttgcc
    24781 aatatcgcaa ttttcatgaa ttaaactttc ctcagtaatt tcatacttca atctgaacaa
    24841 tccaaaccat tttccctaat tttctattaa ataacgcaag ttttaagcat cacaacagag
    24901 ctgctctgaa atgataaaag cagccaagcg tagaaaatcg cgggttttaa gccaagataa
    24961 atgaaatgtc aacaatcctt acttaaagcc tagattaaaa atcacaaatt atcctccctg
    25021 ccgtacccaa caggagagta atttttgaat tcatgcaaca gtcaattaat taactaatgc
    25081 attctagata gatttctttg acataaataa aacaagtctg cttagccatt gctcctgcag
    25141 aggtaaaata tcgaattaca ggttaagata ccaataagac ataatcctta gctaagagat
    25201 ccgcatgatg tggtgttgta aaaaatgcat tcaactcacc ttgaggatta aatagcatta
    25261 cagccccact atgttgtata tcataatttt caggctccga actctcttta ttgtttattt
    25321 ttgcgtaagc aatgcccatt tcatgggtca ttgatttaat tgaatcctct tctcctcttg
    25381 caccataaaa tgatttatgg aaagaagtga cataactacc caatttatca agactgtctc
    25441 tttgcggatc aatagatatc atcacaactt gaggtaaatt tttcacccct ttcttttcca
    25501 gaattcgata catttttcca agctctgcca tggtagtcgg gcatagataa ccacaattag
    25561 taaatccaaa aaatactaaa gtccagcgcc cctgcaagct tgagttattg aaagccttct
    25621 gatctgtgcc tgttaagcta aaattattga ctgttcttgg attttcaaga taagtcccgt
    25681 gaaaatccgc cacattgatc ttctttttaa aatgaaaatt ctgagagata taaataccag
    25741 ctaataagct gaccagggcc aatacagtga ttgttacagc gccccctttt atctttatgt
    25801 tcattctaac cccttaaaaa tagtgatcta ctaataaaaa gacaaacaac agcattaggt
    25861 acagaataga aaatcggaat gtttgcatag caacgactgg tttatcagta aaatacaact
    25921 tatgtgccca aaccaaaaac ctaatgccca aaaccaaagc acctagcaaa taaatccagc
    25981 cgctcatact tacaacaaaa ggcaaaagac tgactaccag caataaaacc gtatagagat
    26041 aaatatttaa tttagtgaat tgaataccgt gtgtcacagg taacatagga atttcagcat
    26101 cttgatattc tttatatcga taaattgcca aagcccaaaa atgaggaggt gtccatgtaa
    26161 atataatcaa tactagtaac aaggcctgag gatccaattg gccagtaacc gcagtccagc
    26221 ctaataaggg gggcgcagct ccggctaggc ccccgataac aatattctgc gaagtggctc
    26281 gttttaaata ccctgtgtaa acaccagcat aaccaatcag tgttacaaag gtaagtaatg
    26341 ctgttaattg gttcacaaac aggataagaa gacttaatcc cattagcccg ataatgaccg
    26401 cgaaccataa ggcttgcttt accgacacac gtccataagc gactggtctc ttttttgttc
    26461 tggccataat cgaatcaata tgcctgtcta ccaaatgatt aattgcagca gcgctgccag
    26521 cgcacagtcc tataccaagc aaagtaaaag cgattaaccg caagctcacc cagccaggag
    26581 ctgctaaata cattcctaca atgacagtga gtagcatgag caacacgacg cgtggcttac
    26641 acaattccac gtaatcacgc caatccacag gaagtcgagc tgcgtattca gtacgcatgg
    26701 ctaacctcct tatcttgtgt aaaatgcaaa gaacaaaatg ccaccgctaa taataatgcg
    26761 gctacaccat tatgagcgac cgcaaccttg agtggtaata gatgaatcac attcattatc
    26821 cctaaagtga attgaagtaa tactaacata gccatgataa tagcagttgc tcttaaataa
    26881 attgaatgcg ttttgaatac gattattgat gacagagtca gtagataagt ggcagtcaat
    26941 atagccccta ttctatgaat aaattgaatg ctggccctaa cttcactctc aagaataccg
    27001 ccttggtaat tggcgcctat cgtagaaaat aaattaaatc cttttgcaaa atgcaattca
    27061 ggaaaccagg ttccgttaca taatggaaaa ccaatacagg aaatacctgc ataattggca
    27121 cttacccaac cacccaaagc aatttgcaac aaaatgataa caattcccaa acgcaaccac
    27181 ggtcgccatt caggtaaatt ttgaccctta agctgggcta tctgcaaacg aaaacggcat
    27241 aaacaggcaa cgattaacat gcctcccagc aaatgtccca tgacaactac aggcagtaat
    27301 tttaaagtaa cagtccacat tcccaatgcc gcctgaaata ccacaagcat aaataaagcc
    27361 aagggaagat gccaaggcaa atcttgatcc tggcgacgtt tgaaccatac ctggaaagca
    27421 atgaaaaaaa tcaacagggc taacgttcct gccgcatagc gatgagccat ttcggtccag
    27481 gctttcttgg attcaatagg aaggtccgga tattgtgatt gagcttcttg caaaccttta
    27541 tctgctcctg gaagaaccat gcgaccataa caacctggcc aatccgggca gcctaagccc
    27601 gcatcagtta aacgagtata agcgcctaac ataacgacaa aaaaagccaa aaaaacagca
    27661 agagtcgctg ctaatcgtat tgaactatta gacatcaatt tatccgctct taatttcagt
    27721 tgtattgagt aaaagtttta aatctttata tatatcacct gggttacacc ctggttgata
    27781 actcagtatg aggtaattat ccgggtttgc tataaaaatt ttagcttcag gatataaagt
    27841 cgacaattta gacatttcag ttaatgataa ggcagcaaca tggatatctt gttcttttaa
    27901 aatgggtttt aattcctctg cgatcaatga atctttgttg cttagaagca accattcatc
    27961 tacctgatat aattttctcc caaaagaaag acgaactctt cccaacatat taagctgctt
    28021 aagacaagtc ttgtcacaac cattaggatt ccaatagata atgcgccatt ttgcactgct
    28081 atctaatggt tttagagcga tcggtggaga aagtaaaatt cctttattta ctttagaggt
    28141 tccaagccag gaaggatttt tatagaacaa ataagcggtt attcctggtg cagcaaaaat
    28201 cactaccaag agtagtaatg tcatgtattt atatggtttt ttcatttttc ttcaaattaa
    28261 aaatcacgaa tataatcaga ataaccaatg ccatggcaaa ccactgtatt gcataagcaa
    28321 agtgcctttg cgggggcatg gatactatct cccattcacg tacaaaacca aaaggctcat
    28381 tcttgtcgag gcgaataata aacggataga cctttttttg caaaagttgt ttcagtattt
    28441 cttcatcaat cagctcaaga agagtcacct tattctcttt ttcttcaaaa cttggcccta
    28501 atacccattg ttttttactt ggaaaataaa ccatgccaaa tagtttaaat ttgccgttgg
    28561 gagtctgaac attaggaaaa gtgcgtcgag taatgtctcc agaaacccac cctctatcca
    28621 ccataacgat agagtcatca tcaagaagca taggagaaac aacgtcataa ccaaactgat
    28681 gttgataatg ttgattatca agcaaaaata actttggcag aaaagctcct tcaatgctga
    28741 ttcgttgata ttgctctggc aatttttgcc ctggctgcca agttatgggc tcctgctttg
    28801 ccagcgcttg ttgagcggtt atcatttcaa ttttttcatc ggctctgtgt atttgccaaa
    28861 atccgaggct aatgaataaa aaaaagcaaa cggccgtaag aataagcatt ggccaatttg
    28921 aggtgaaacg aaaattaaaa caggttaaac taggcatctt ttatcaacta ctcatttggt
    28981 tagctaacat tcccctgaag ctagcaatgt caggagtata acaaatgttc actaaaatct
    29041 tcatcatctt tgtcatgatt cttattgtgg cctcattagc gagcgggctc ttttacctgg
    29101 tcaaggacac tgacaaatca aagcgtacag taaaagcgct cactgtgcgc attgcaattt
    29161 cattatcttt atttgtgttt ttatttattg ccttcaaatt gggattgatt cagccacatg
    29221 gaatttagct tttggctttt aataaattca cctcaagaga taggatcatg gctgagataa
    29281 tgagatattt ttaaatagtc atttcagcca ccttcaggaa attcccccta atccacgaga
    29341 cgacaaacaa cgagaaaaaa tctcgcaccg agcatcaatt gaccgtaaac caataccacg
    29401 gttccagaca ggaggggcta ctctcttaca ataaaatttc gaaattgctc aatatactat
    29461 ctatactttt gttgtaaccc tcaaaaggga tggtgagcat catgatgata gttctgtttt
    29521 tattttattt cataatagga ttacaaatta tatttaaacc caataaaacg ataatactcc
    29581 aatttctttt cagtttgttt cttatactaa tcagcttcaa ttatcacagc catctggtga
    29641 acctatgaat aaacaaatgc aatggcatct tttcgggaat acttcggggt tattgataat
    29701 ttgttgcctt ttgatcttgg ccttcctgga tcaattcatc ttttatgaac taccctgccc
    29761 tatatgcatt ttgcaaagga tttgctttgc tgcagtaggt ctctgttttg ttatgaattt
    29821 aatgctgggt attagagctt cacattacgg gctgatgctt ttggcaagtc ttttagggct
    29881 gtctatctcg gttcggcaaa tgtatattca tttaacacct ggtgatcctg gttatggcgg
    29941 cctctttttg ggtttgcatc tttacacttg gtcagcaatc atatttttag tcattatctt
    30001 attaattgct ggggctctaa tccttgataa gggcctcatg ccagattata aagtacgaag
    30061 caagtcagca aaagtaatga tatgggtatt ttttatcttg attcttgcca atggaatcag
    30121 tacattcttg gaatgtggac cgcacgaatg tccagataac ccgagcaaat attatttact
    30181 aaataataat taaggatctt gaggatttaa aatgcgtctt ttgtttatca gtttaattct
    30241 tgccagcgga tatttacagg ccgatacaat aaataattat atgaatattg ctaacaatat
    30301 tccgcaaatg gaaatgaaag cagaccctca agctcaagca tgggccagat cggcacgtca
    30361 tgtacttacc atcaccagtg aaagtattgc tgaaacttta atccaggcga atgaagccgc
    30421 aaaaacacaa ggtaaaccta tcttttgctt accacaaggt gtgcaactta attcttttgt
    30481 aatgaacgag ctcatacaac aaacctataa agaaatatcc agtcaacaaa gcgacaaaga
    30541 taaaatgacc gtttctcaag tggctttatt agggcttagc aaaaaatacc cttgtgaacc
    30601 cagtcctcag gagaaacaaa ttcaacatgt ggcttccctg ttatcgccgc aatagcgccc
    30661 gcagcaacaa agtcccggaa tttttttcta catccgcttc tttaaaccag acgacatgtt
    30721 ctgaaaattg ttagagtggg catggcaaag caaagttggc agagcgtcaa acactgtgaa
    30781 ttcccttgac aatgtttgca aaaactctgc cttgctggtt ttttattgat tttagtgttt
    30841 attaagcgca actttagctt ctttacaatt acttactaaa ttcctcaaaa gcttccaatg
    30901 cttctgcagc atacattaga gaagggccgc ctcccatata aaccgccata cccaatgttt
    30961 ccagtaactc ttctcttgtc gcttgcagtt tcactaaagt ttgtgaatga aaccctatgc
    31021 atccggggca ttgcttggcc acggccaacg ccatagcaat taattccttg gtttttttat
    31081 ccagtgcacc gtctttcgtc gcagcttgtg ccaaagaaga aaatccagtc attaactctg
    31141 gcatctcttt gcgaaacttg gctaattgcg tagtgatatc ttttgtaatg tgtgaaaatt
    31201 tgtctgacat gaggctctac tcctttcctt attatatttt ttcaagcgtc tctaatttac
    31261 agcataatat tgggatgcaa aagatgtatt ccctaattta acgtgagtaa tatataagat
    31321 aggtcgttat ctttaatcgt ccaggctgag gatatcgcct aattctaata caatccctat
    31381 cgataactca cgttaattta agaagtggcg atttcaggat attgctcgat tgagcgtttc
    31441 cttagccagg gtaagtcttt tttcaacatt ccctaattcg gtttgtaaat gcaaaatagt
    31501 tgcaggatta gagcaggact tcatttgctc taaagttttt ctcatggctt gatgttgctc
    31561 ttccaaggca cagatattct cctccatggt tttaatccat tgttgtcttg tactaagagt
    31621 tgtcaaatca aaagaaattt ttgattcagg tttattattc tttttgtcca tttgacataa
    31681 aatgaccaag acattaattt ttttctggat acgctctgcc aaaaagcatg caattgactc
    31741 atccttatgt ttctgtaaag cgtgaatatc atccctgatc tccttgatac aggcaggtcc
    31801 attgaattct ataacggaag aaaatatgcc tttgggcaaa ttatgaattg aaatagaaga
    31861 actcaatcca ttgatcttcc attccagctc tggcaatcgt gcagatagtt cttccaacaa
    31921 ttcgtcaata tagcccatct tttgaaaatt acataaacaa ctagggttat taaacttaac
    31981 ctgtcttgct aaattataca gcaggttcca aatccgcctc tttggcttgc aaccaactgg
    32041 ttataaaagg aggagcacac atgagcagca agccaataat caaggtcaat tcataacgtg
    32101 ttgcactgcc gacattaatc ccctctggag gaataaagct aactgctaaa gtggtaacca
    32161 ctccaatgat gccaatgcct gcaacaaaca acattcctgc catacctcct ggaattttaa
    32221 aaggtctggg atgttgaggt tggcttatgc gcaatttaat tgctgcgaca aacatgatca
    32281 aatacatcag catgtataat tgagccgcca atgccgttaa gagccaatag gatccattga
    32341 cactgggcat aaataagaat aacgccgaca atactgtgac tatggttgcc tgggtgtata
    32401 gcatcatggc cggagcacct tttccattag tccgttgaaa tacctctggt aaattccctt
    32461 cttctgcggc aactaataaa cctttggtag gggcaatgat ccaattactc acaccaccaa
    32521 gtcctcccag aaccaacatc acagctacca caggcatcat ccaatccatg tgatatttaa
    32581 ggaaaaaggc agaaaaggct tgcataattc cggcaaccag attgatatcc ttttgtggga
    32641 gtactaccgc aatcgctaat gaacctaaaa tcaaagtact cagtatgatc cctacagaat
    32701 aaaccagggc tttagggaaa gcatgctggg gatccttcac atcattagca tgcactgtag
    32761 caatctctat cccgcaaaat gacaatatga tggcggttaa tgaaacccac atgcccttat
    32821 ccgataaatg gggaacaata ctgggcatgt caaaactgat ttgcaagggg ttaccttgag
    32881 ctatccaaac agcacccaaa ccaatgatca aagacatggg taataataat cctgccaaag
    32941 aacaaatatt actgaacatg gccgatgatt tcatgccgcg caaattcaca atggttgctc
    33001 cccaaaagca actgacaatg actgcccaca gaaaataagg attggtagtt aaagagggat
    33061 taataagata acctattgtt cctgcaacaa acgatagaat cgtgggatac caaatcacat
    33121 tttctatcca ttgcaaccaa atggctaaaa accctgattt tttcccaaaa gcttccttaa
    33181 cccagatata aacgccaccc tgcctggccc acccagaagc taactctgcc gagaccaatg
    33241 cggttgggat aaggaaaaat aaggcaccca ggataaaata aaaaatcaat tgactcccaa
    33301 ataatgcagt agctggcaaa ttacggatac tgtccacaga gccaacggta atcatagtca
    33361 agctaaatat agtgagagaa tgttttttat tgctcattgt attccttcag tgaggactat
    33421 ttggaatcaa aagtttatta taaagaaata agcttaagga aaccttaata atcgaataaa
    33481 aaataaccaa atatacccct tagtatactg acctataaaa ttgacccaac aggcattgag
    33541 aattttcatt tcagtacagg tccgatgcga aaacatcata tgatgttatg tcattaaaaa
    33601 ccagtattct gtatataaaa ttaataacaa gaatctttct aatgcacatt aagtggtttg
    33661 tttactaaca gggcgcaaaa ccccaaggtc gagacaaaaa gcgtgaggtg ttaaaaagac
    33721 taattttatt ctcctatttc ataccaaaac caggttgctg tgattgataa gaaatagttt
    33781 tcactcctct ttggaacaaa gtaccttttg tctcagaaaa tttaaatcga cgagaggttg
    33841 aattctcctt cgcacaagct tctttttcca atctctcctc gatcatttgg attgcaaggg
    33901 tatgctcgtt tttaagagca tgatcaagtg cggtcaaacc gtcattatct ttaatagaaa
    33961 gagaagcgcc ttttgcaaat aacaaattaa ataatttttt atcctctgtc actacagatt
    34021 ttttacaggc gtacatgagc ggcgttctcc ccttgttgtc ttggggatca attttaattc
    34081 cacgtatgtt caacagatct tcgactatac cactaccacc ataccgacag gcaatatgga
    34141 gcaaagtaga agaatcgctt ttacgctttt ggttaactct atgtcgagaa gtactcagca
    34201 tggcacgtac tacgtctctt cttcccaaat agatggcggt aaggataggc gaatcatcac
    34261 ttagccgttg tcgagaaaaa attgaccagc cagaatgaat gcattttttt acagaattca
    34321 tatcaccctg agtagctgcc atggaaaaaa aagccaatcg ttcagggtaa gaagttttgg
    34381 ttctgactgg aaacacaggg aatacattct gcagtgctct tgatagtttt tcttcaggat
    34441 taactgaaac aaaatcggta ttcacggtca atcgacttcc ctgaaggttt tcggcacaaa
    34501 cacgatacaa ctctttaacc aggtttcggg acgagaactc aaagtaaggt ttatttttgt
    34561 cttgttggta taaatcattg atgttaataa accgccagac attaccagcc agtttaaatc
    34621 ccatggcgtg atctgcggta ctgataatta ctgcaacatc ctctctgatg cctgttcttt
    34681 ttaataattc aagatactct gttgcttcat cctgatttaa aaaactatga gtcttataaa
    34741 aaatgtgctt tgcaacggaa gaatcggcat ttatggcttt caaaatggaa ttaatatcag
    34801 gctgccatac cagttttctg tatttctctt tatattgcaa ggggtcttga gcaacacaaa
    34861 ttttctttcc taactgaggc aaagtgtcaa taaccatctc atcgttgctt aatgattggt
    34921 tcctttcttt cttttcttta atttgttgta atgttttagg caaaccaaac tggtgggttc
    34981 gtaaatgatg caattggcgg taaaaaaagg attctttacc ctgagccact gccaatgccc
    35041 aattgagagt gaagccataa cagactcctt caaacttggg atgacccaat tgtgttaaga
    35101 cattcgtaat accatcatga gttaattcac tggtgtcctg ttttttggag atcaaagaag
    35161 tagaatacga caggagcttg ggcatgagtt gatacactac caaaccgcaa actagcataa
    35221 tacaaattaa aacataaatc atatcccttg aacctgatgc tatccataat gacttgagta
    35281 taaaattaaa ccatcataca agtcgagggg atgagatatt cgattagctt caagatcctc
    35341 caaacaagtc aggcttactg ccctattatc cctctattgc accgcatgat acctctctga
    35401 tgctccataa tgaaaacatt taattcttct taagaaaacc ttaatgttaa ggctttaaac
    35461 tatgggtcca aaatatagac aattactttt ttttaaaaaa tcattggagt cccttatgcc
    35521 taaagcattt acccatttgt gtaaaaaagt caatctcagt ttatataacg atacattgac
    35581 tgtctaccct aaatcatctg tactgccaaa acctattcaa atacgtagtg agcttgatct
    35641 tcttatggtt ctgttgtttt ctcaaatgga ttgcaatagc gcacaggttg atcaattttt
    35701 tatgatgcgt tcgaatgaac tcggtgtcta tcgctttata catcgtgacg acactgatca
    35761 tgatttgtat caccaagatg aatttagcat ggacgataac cattatcatg ctcaatttga
    35821 caaggctatt gacgagatta aattggaaaa tattctggga atactagaaa agcattctct
    35881 tatttcgtct gaagaacacg taagctttat cgaagcctac cataaagcca atactctcac
    35941 cgaagatatg ccaaaactgg aaacaactct gtctagtcct ttgcctatat cctctccttc
    36001 agttcactca ggcgcaaaaa gacctgttgt gaaagaaaaa gaagtagata ctccaacaac
    36061 ttttgcagga ctaagagtag gatttttcct tacttcaaca aaaacaacaa caaaaccctt
    36121 atccagatct catgatgatg agaagaaaat cggaacgaat cggcatgagt tcaattaatt
    36181 gatatagttt gataaaccat agtaaagatc gttctattgg tttacttaat atgacaattt
    36241 atggtgattt acccaaaatc taaaaaactg tagggtaact aataaattac tctacagctt
    36301 ctaattacaa aatcatatag ataaccctaa ttgattcact gttatcaatt actgtgactc
    36361 attaatacgt ttttctctgt ggtatacttt taactcccta tgttatagta gttatttttt
    36421 aggtactact tattatgttt gaaaatttaa ccgaacgctt aacccggacc tttaaaaatt
    36481 tgaggggtca aggccgcctg acagaagaca atatccagca tgccttgcgt gaagtaagac
    36541 tgtctcttat tgaagcggac gtggctttgc ctgttatcaa agagtttatt gagcaagtaa
    36601 agcaaaagtc tcttgggcag gaagtcttaa ccagtttaaa tcccgatcag gcattcatta
    36661 aaatcgtgca tgatgaactc attcacgtca tgggcgatga gcgggttgaa ctggattaca
    36721 aaacccaacc gcctgctgtc tttttaatgg ccggtttaca aggttctggt aaaacaacga
    36781 gtacagcaaa actcgcgcgt tatttgaaag aaactgaaaa taaaaaagtc atgatggcaa
    36841 gcgttgacgt atacagacca gccgccatac accaactgaa agtgttagcc gaacaaattg
    36901 acgtcgcctt ctttgattca gaccctaatg agcaacccat aaaaatagct caaaaagcat
    36961 tggaaagcgc cagaaaacag tatgttgacg ttctcattct cgatacagca gggcgcctgc
    37021 acgttgatga agacatgatg tctgaaatca agtctttgca taaagcagtc aatccaatcg
    37081 aaaccctttt tgtcgttgac agcatgacag gacaagacgc tgccaatact gcaaaagcat
    37141 ttcatgaagc cctccctctg actggtgtta ttttgaccaa aacggacggc gatgctcgag
    37201 gaggagcggc gctatctgtc aaacaaatca cagggaaacc tataaaattt atagggagtg
    37261 gtgaaaaaat agaagccttg gaaccctttc accctgaacg aattgcatca agaatattgg
    37321 ggatgggaga tattttaact ctcattgaag aggtagagcg caaagccgac aaacaagcca
    37381 gcgaaaaact ggccaaaaaa ttaaaaaaag ggaaagggtt tgacttagaa gatttcaaac
    37441 agcaattatt gcaaatgaac aatatggggg gtatctccgg gatgatgagc aaactacccg
    37501 gaatggggca attgccaaag caagccatgc agcaagtgaa tgacaaagcg atggctcaaa
    37561 ccattgcaat cattaactct atgactccca aagaacgccg tatacccaaa attattgtag
    37621 gctcacgtaa aaaacgcata gccttaggct ctggaactca aattcaggat gtcaacagac
    37681 tattaaaaca atttgagcag atgcaaaaga tgatgaaaaa atttaccaaa ccaggcggaa
    37741 tgcagaaaat gatgagagga attggtggca tggccggatt gaaaggcatg tttccagatg
    37801 atttaaaata attcgtaaga attacttcac atgtcgatgt aattttcgta caatacacgg
    37861 catttttatg taaccactct aacagtagag gaaaataatg gtcgttatac gtttatcacg
    37921 agctggcgct aaaaagcgtc ctttttatca catggttgtt actgacagcc gcaaacgtcg
    37981 cgatggaaac tacattgagc gtattggtta ttttaaccct gtagcacgtg gacaagaagt
    38041 aaagcttcac attgatatgg ataaaatgac ccactggcaa aaagttgggg cccagttatc
    38101 cgatcgagtc agtgccttgt taaaagaaca tagcaaaaaa tcagagacag ctgcttaacc
    38161 aaagtgaata acaagacaaa ttgggttatc atcggccgtt ttggccgacc ccatggtatt
    38221 aaaggctttg tcacggtaca ttccttcaca gaccctgctg ataatatctt gcggtacaat
    38281 gactggcacg tttttttaaa taaacagtgg cagcccctta agttattgac aatagaagta
    38341 agaagtaaag cgattattgc tcaaattgaa gggtacccag agcgtgaatt agtctctgca
    38401 ttaaccaatt tagatattgg tgtgcaagaa tcacagcttg cagcattggc accaggtgag
    38461 tattactggt atcaactgat tggcatgagt gtcataaact ccaaaggcga tctttttgga
    38521 aaagttgttg aaatcatgcc tactggctcg aatgatgtac ttgttgtgga aggtgaaaaa
    38581 agacatttaa ttccctatct tccaggacaa tttgtaatca atattgatga aagccagcaa
    38641 gtaatcactg ttgattggga tatgaatttt tagtggcatt acatcttgga gtcatcaccc
    38701 tgttgcctga aataatacaa ggcatccatt atggggtaac cggccgagct attgaacaag
    38761 gcttggttaa aattgattgc tggaatccta gggattggtc atcaagacct tataagcaag
    38821 tggatgacaa gccctatggt ggtggaccag gaatggtcat gatgtatgag ccattgcatg
    38881 ctgcaataaa gcatgcgcgc agtgaaatga aggaaaactg caaaacaatt tacttaagcc
    38941 ctcaaggcaa ggtagttaga cagaacgatt taaaacaaat agctatccaa aaacaatcct
    39001 tgctatttgt tgcagggaga tatgaaggaa ttgatgagcg aatcattaat caccacatag
    39061 atgaagaatg gtcgctaggg gattttgtat taagtggcgg tgaattggcc gcaatggtat
    39121 ttattgacgc gataattcgc ttgatacctg gaagccttgg gcatttgggt tcagcagaac
    39181 aagattcatt tatgaatgga ttactggact gcccacatta tactcggcca gcaactataa
    39241 atggactgga tgtacctgat gttttattag gaggaaacca taaagaaata gaacgctggc
    39301 gtaggaaaca atccctgggt aaaacctggc tcaaacgccc ggatttactt gagaaaatac
    39361 aattaagtga aacagacaag caattgcttg ctgagtttaa atgcgaacac ggcgattcct
    39421 gctgaggata agccaatttg aggagtagtc tatgactaat attattgacc aaattaatgc
    39481 cgaacaaatg caaggcaaag aaattcctga tttcaaccca ggcgatactg ttctggtcca
    39541 agtaaaagtt attgaaggta accgtgagcg tttacaggca tttgaaggcg ttgtgattgc
    39601 caagcgtaac cgtggactaa actctgcttt cactgtacgt aaaatttccc ataacgttgg
    39661 tgtagagcgc gttttccaaa cctacagtcc tattgttgat agtattacgg tgaaaagacg
    39721 tggtgacgtt cgtcgtgcca aattatatta cttaagaaat cttgccggtc gcgctgctcg
    39781 tattaaagaa aaattatctg ggaagaaagg cgattaattc ttgaatggta aaatgctcct
    39841 cgtgtctatt tttaatactc ctgtgggtaa cctggaagta gcccatgatg agcattttat
    39901 tttcaatgcg tccttcactg acaaatcggg cgctccagcc caaaaccaat tggcccattt
    39961 aattgccagt gaattgaaag cttacttcga cgatccccat caccgttttc agctccctct
    40021 gaaacctcag ggaagcctct tccagcaaaa ggtttggaat acgttgttag tgattcccgt
    40081 tggcagaaca gttacctatg gagaacttgc tacaaaactg caaagtagcc caagagcagt
    40141 tggacaagcc tgtaaatgca acccactggc tttatttgtt ccttgtcata gagtggtagg
    40201 aaagcatgat cttggaggat atatgggcaa tccaaacgcc atttcttata aaaaaaatct
    40261 gctcgctcat gaagcaaaaa tgatctgatg aattaagttt ataaaaatcg atattacata
    40321 gatcagaact tacaaaaaca ctaaggaaaa aaatattctt aacgaagaga ttagcaagtc
    40381 gtgtgagatt aaattttggg attcttatcc gtatcagaat tgatattcaa aggtggggaa
    40441 ggaggttgta atatactaaa aaatctggcg cttaatccag ttaatattcg cacagtatta
    40501 tcgataaacg gcagcggcga aattttcttt aaaaactcct cttcgaactc agcaggctgc
    40561 atttcccata gcttttttac atacacggct atttcatcat ggacatcttg agttaattcg
    40621 gtttgctcat ggagatactt ggctaatcga acaaccaagc tgatacgaga agattttaat
    40681 tgatcatttg gcaagtttgc agactctaat aaactaagat aatcttcaat taaatatttc
    40741 aaataggtta cagaataaat ttgatcaaac gctttagaaa ccgctgaaga gtctgcttcc
    40801 ttaacccaag taaaaaattt atcttgctct tgccctaaaa attcgacaaa ccgttgcttc
    40861 tcaagataag cattgttcat agtcattaat tggagcactt ccttacttga taattttttt
    40921 gaagtaatat agtgctcttg cagattattt gttaaataca ccatacaggg aaaaagagcg
    40981 atagaaattg tagtgagcgc ataggataat ccctctctta aaggcctgga ctccatttca
    41041 tgaacggcat acaaaatagc aaatttttta atgtcatcca aacaaaagcc attatttaac
    41101 cattcaaata tgagcaaggt taccatttca tcagcttctt gatttttgtc gttcttacca
    41161 taatattctg caataaattt tggccactgc agaaatagaa ggtattttcc atcacgtaaa
    41221 gcttgaagat attcctttgt tgatgaaagc atagttatcc attaccaaaa tctgattatt
    41281 caatttataa cattaacaat actaaaaatc ttgtgaaaaa ttatttcatg ttaaacaaca
    41341 tacgcacatc aattgttatc ctatacttaa aataaaacga caaatcaggt ggggaattct
    41401 atgaaaaata aattagcaat aagtatatta ttattcctgt taccaacggc tgctgccatg
    41461 gctaattgtg atttaaccaa gtttcgatgg gattgtgatc ttccagtaca ggttaagccc
    41521 agtccgggcg cttcctctct agtgtattgt ggaagctctt acggctatat cacaaagcag
    41581 caatatgata ttctggctcg ctatcaacgc gccagtgtca atatggtttt ggatatcaat
    41641 ggtgaataca ttgatagccc gtgcatagga gcggaacgtt aaaatttttg cagcaatgac
    41701 taacaataaa caatgagaaa atgaaaagga atattattat ttccagcaag ttgcttattc
    41761 aaaatcaaaa atctctggat ttttgggttt tcaatgcact caagtatcaa tatcataaat
    41821 attacttctt ctgtataatc gaaaaacaaa tcaataagct attttgctat gcacattcaa
    41881 attgatggca ttcctataga aatactgaga aaacccatta aaaatttaaa tttgagaatt
    41941 tatcctcccg atgggttagt caaagtgagc gtacctttaa aatatagcga acagttaata
    42001 aaacaacagc tgcaggaaaa aagtgaatgg attcgtgccc aatgcgagcg aattcgcgca
    42061 cgctcttttt atcgtgaacc atcacttcaa acaggcgcaa ccattccatt caaaggtaaa
    42121 aactatttat taattgttga agaacatcat ggcccctctc aagtcctgat taaagatgaa
    42181 ttaatttatt gctatacaaa acctgattta actccacagc aaaaacagaa cattctggaa
    42241 cgctggtata gaaaagaaat gaatcttgtc attcccaggc ttattcaaca atgggaagcc
    42301 ataatgaaag tcaaaattaa tcaatggggt ataaaaaaaa tgaaaacccg ttggggttct
    42361 tgtaatcctc gcgcacaaag aatctggctc aatcttaatt taatcaaaaa gcctttggag
    42421 tgccttgaat acgttcttgt tcatgaaatg gtgcatattt tggaagccag ccataacaaa
    42481 cgattttacg cactcatggc acaattcatg cccaaatggc aagaatatca aaggatactg
    42541 gaaggtacac ctaatgccaa tacgtttgca aggtaatcat cgaattcatt acctcattat
    42601 ttccaaaaaa taattcttgg cgtacaatgc gttatcactt tgcctgattg gtgtagacag
    42661 gttgtttaat ccggacttaa ttgcccataa tatttgtact caaggatttc atatcattga
    42721 tgaatttctg gaagaggcac actactactc tttacgagac atggcttatg aattaaaaca
    42781 gcaaaattta ttcagaaatg ccaaaattgg ccagaactta caagcgcagc aaaatgacaa
    42841 gattagaagc gatcaaattt tctggataga tgcagactcc ccttatctac ccattcaagc
    42901 ttacttaaaa aaacttatct ctttgggcaa tgttctgaat caaaccatgt atcttggttt
    42961 agcagagttt gaaactcatt ttgcaattta tctccctgga accttttata gaaagcacat
    43021 tgatcaattt gaaagtacaa aaaccaggaa aatatcattt gtctattatt tgaatgatac
    43081 ttggcaaaaa gaatttggtg gcgaattgtt actctacaat attgaagatg agcttattca
    43141 aaacatttct ccattgggaa atcggttcgt ctgttttgat agtcaattac cgcacgaagt
    43201 tcacccaact caacgaacac gcttcagtat gacaggctgg atgaaaacac gctcaacctc
    43261 cgtactggcc gacaataaac tgacccaaat aaccccgtag cactttatac gctgttctat
    43321 aaaaaaatga ataattattt tttaacttca ctacagctat tgaactttaa tttaagttga
    43381 tattaaatga aacactatga ttccatggat caggagttaa ttaccatgcc tatcagtaat
    43441 gcctttctta agttattcag tgaatttgtt gataattatg cgcgcaatgg caccccttca
    43501 ttttatcatt gggcaaagga acaaaaccat gaagtcatta acaaattttt ccatgagctt
    43561 ttggaaatta ttaaatcaaa cacagaaaca tttcatgatg tcagtatcat agagcacctt
    43621 aatgctacag tactattatc agcaaagacc aatccctact tactcgattg ttggatttca
    43681 tttattacta aatacaatgc tgaaaccaac agttttaccc caataggggc ttcgatgcac
    43741 attaaagtcg gaccagaagg cacgccatcc gaacggataa taagggagct caaagaaagt
    43801 ctggtcatga cgcaacaagc gtttatttta gctcaaaagg ccaatgctac ggaagttgac
    43861 tctttaaaaa gacaacttga gcaaacaaag caagaaaacg agcgcttagt cacacaaaat
    43921 cagtttctaa aggatcaagt cagtgcagaa aaaacatttg cagaccttaa cccacagatt
    43981 aacaatgctc gacagcaact cagtgcttta acagaattaa tacaaactct aagcgaaatc
    44041 acaaatgcca aacccaaaat agaaactgta tcttcgttat ccattccagc tgttacagaa
    44101 agtaagtctg aacttctaaa ggaaaattca tgctctgtcg cccctgtatc caaggaagtt
    44161 actcctgcaa tcagtcaaga accgaaaaaa agaccagttc taccgccccc caatgatatg
    44221 gatggtcaag cattatcccc caatataccc cctaaagaag atgtgtctat aaaagttaca
    44281 ataccgtcca tgcccaaacc aaattcaacc cagccatctg caaaaaattc aggtttcttt
    44341 tcaggcgatg gttttttaaa agagttaaat gcaaaaatta aagaaagaaa ggaacgccca
    44401 aaagatgagc cacataccac taccgtcgaa aaaaaaaatc tctagttcag ccaatttctg
    44461 ttcctcctga atatagggcg ctcttagcta aaatggagga aattagaaag gcagtgacag
    44521 gcaaatatcc tcataccgat gattcatcgc cagagtcaac tcctccttca tccccagaat
    44581 taacccgttg gtagtgaggg atgaattaaa atccataggc tactgtagaa taaaaattat
    44641 ggtgtataat agcgttttta cttaagtaat aaatcatgca tgccttagaa attaaacaac
    44701 tttcaaaaac ctactcgaat ggagttgaag cgttaaaagg cattgattta acagtgaata
    44761 ccggtgattt ttttgccctg ttaggagcaa atggcgctgg caaatcgact accattggtt
    44821 taatcacatc tttagtcaat aaaacctcag gaaacatcaa aatttacggc ttcgacctgg
    44881 atgcttatcc tgaaaaagcc aaatcttgtc tgggacttgt ccctcaagaa gtcaatctga
    44941 atatttttga aaactgcgag caaattttac tgaatcaagc tggctattat ggcatttcaa
    45001 gaaaaaaggc tttacccaga gcggaagcct tgctagaaca actaggactt tgggacaaga
    45061 gacgttctat agtacggcat ttatcaggtg gaatgaagag aagacttatg attgccagag
    45121 cgctgattca tcaacccaaa ttactgattc tggatgaacc caccgccggt gttgatatag
    45181 aaataagaca cagtatgtgg gattttctag cgagaactaa tgcagaagga acaacgatta
    45241 tcctgacgac tcattacctg gaagaagcag aacaattatg taaaaacatc gcaataatcg
    45301 ataaaggtca aattattaaa aatacctcaa tgaaagcctt attacaaacc ttacgccatc
    45361 aaacctttat ctttaacaca gaaaacccta ttgattattt gccggatata cacccgttca
    45421 atgtgacccc gattgatcca actacttttg aattgcgtgt tgacaataac ttcagtttaa
    45481 atgaagtatt cgctgtatta acaaaacagg gaataaagat tcacagcatg cgcaataaaa
    45541 ccaaccgatt agaagaactt tttatggatc tgattaaaaa tgggatttaa atatcaactt
    45601 attgcccttt ataccctagt aagacgtgaa gtggttcgta tgtttcgcat ttcgagtcaa
    45661 gtatttttgc cgccagtaat cactacaacg ttatattttc taatttttgg cagtttaatt
    45721 ggacctcgta ttggagatat tgagggcgtg gattattcac aattcatagc acccggatta
    45781 attatgatgt ccgttattat taattcctat ggaaacgtct ctaattcact attcagcgta
    45841 agatttcaaa aaagtataga ggaaatgctg attagtccca tgcataatgg acttttattg
    45901 ttaggttatg tgataggagg cgtgctgaga ggattaatcg tcgcaattct ggtttttgtt
    45961 atctctacct tttttctgcc tgttgaatta gatcatttac caatgaccct tctagtcgta
    46021 gtgcttgttg cagccatatt ctcactggct ggatttacca atgccatggt cgctcgaaat
    46081 tttgatgaca tcatgctgat acctacattt atcctgaccc ctcttactta cttgggagga
    46141 gtcttttata gtattaatat gctgcccccc ttttggcaaa aaatctctct tttcaatcca
    46201 attttataca tggtaaacgc tttaagacac accatgatcg atcaggaaga agttagtatt
    46261 actctggcta tgattattat tttcctgatg ttaattgctt tgacagtagg aaatatgatt
    46321 cttttaaaaa aaggagtggg gtttcgcgaa taaaaaaccg aattccctgc aaaacggatc
    46381 tatttgcgtt taaaacccaa tgcttccagg cgctgctcaa tgctaggatg agttgaaaag
    46441 gcattattta gcgattttac aggatcaatg tgagaaggat caaaaagata ggaagcttgc
    46501 cttacctgtt catgaggggt atctccataa gcttttgcat attgctccgc attctgaaca
    46561 tggtcttgat taattttaag caaagccctg gccataggct cgttatctct cattaattca
    46621 acacaaccgg cgtcagccat gtattctctg gttcggctta aaaagagagc tagcaaaaca
    46681 gtaataatag gtaagacata tcttaagatg ataataacca ttaacaagcg gttatctcct
    46741 cttctgtctc gcttgtaaat tacggaataa aacaggatat caatgacaat taataaaata
    46801 ttactgagca ctgcaaccat gagagtcaat ttaatatcat gatgtctgat atggcttaac
    46861 tcatgagcca ttacagcctg catttcagcc ctgtccagtt tttccattaa gccacgagta
    46921 atcgcgacca tcgccgattt ctcactgtaa ccactggcaa aagcattcat gtaactggct
    46981 tcaataatgt aaactttagg catgtattgc aagcctgatg cgactttcat ctcttcgaca
    47041 acattgtaca attgtttttc ttgtaagcta cgagccgttt ctggcgttat ttgatagtac
    47101 tccgtcccca ataacataat gcggtcataa agagcataag taattaacaa agaaatccct
    47161 gcaaatgcga tcattaataa agtagcaata ggcaccactt taaaagtgat taaagcatgg
    47221 ataatatttt ccaaagaggc atgaggatac atgccttgta aaacaaatgc atcgactatc
    47281 aatcccacaa agatataaac tagaaaaaag actgcaatca cccttctggt ctttttctca
    47341 tttcgcctca attgagcacg ccaatctcct gctgtggcat gataatctgc aatccccatg
    47401 gtatatagct caaagtttca cagtgtaatt ttcttttgct tgaatttgct cttctggaag
    47461 cccccaatag ataaattctt tatccaaagc agaagaaaac attgccacaa ccatagattc
    47521 aaaaaatgat tttttcttgg cgttataacg ttctatccca tcattataag cttgtttcgc
    47581 ataagctaac ttgttttctg tattaacaat ttcctcttgt aactgcatga cattttggct
    47641 agctttcaaa tcaggatact gttcaaaaac caaattcaaa ttggatgcaa tttttgaaat
    47701 tccattttct gcagcaattc gaccttgctc atctcccgca tctttagcag cctgggcctg
    47761 attacgtaaa gcaacgacat cctttaatgt tgttttttcg taatccatgt attttttaac
    47821 tgcttcgatc agtgattcaa aaactttaaa tcgcctatcc aattgaatat ctatttgttt
    47881 tttgttgtta ttaattgcct ctatcaaacc gattagagtg ttatagatgc taattgccca
    47941 gacgaatgcg agtgcgataa taattaaaat tgcgataaaa aatgttttca tcttcctatc
    48001 ctgtgaaagg ttccttccct aatttatagc tcgaaatttc agaaaataac agctaagata
    48061 agaaaaaaga aacaactgaa taacgtgttt tttgtcgcaa aaagagattc aggttgcctg
    48121 aaaaacaacc tggatagtac atcaagtata aagacaaatt gataattaat gaactatttg
    48181 catttctgca atagaagctc atttgatttt gataaaattt gttttaaaaa ctgacctgta
    48241 aaagatcgag agttttcagc aaccatttcg ggggttcctg ttgcaataat ctttcctcct
    48301 ttactgcctc cttcagggcc aagatcaacg atccaatcag ccgtttttat cacatccaaa
    48361 ttatgttcaa taatgacaat ggtatttcct tgctctcgca agcgagtcaa cacagttaat
    48421 aactgtttcg tgtcatgaaa gtgtaatcct gttgtgggct catcaagaat atacagtgta
    48481 tttccggtat ccctttttga taattctctg gccaacttga tcctttgagc ttctcctcca
    48541 gagagagtag ttgcactttg tccaagcttt atataagaca aaccgacatc cattaatgtt
    48601 tggcattttc tggcaataac aggaatggca gcaaaaaaag cgcacgcatc ttcgacagtc
    48661 atgtccaaca cttcatgaat atttttgcct ttgtagtgaa tttccaaagt ttccctattg
    48721 tatcttttgc cttggcatac atcgcaagaa acgtatatat caggtaaaaa atgcatttca
    48781 accttgataa gaccatcacc ttgacaagct tcgcaccggc caccttttat attgaaacta
    48841 aagcgcccag gttgataccc tcgtgctctt gattccggag tccctgagaa taaatctctg
    48901 atatgagtaa ataaaccagt ataagttgcc ggattggaac gcggagttct gccaattggg
    48961 ctctggtcga tgtcaataac cttgtcacaa agatgcagtc cagaaatgtc cttaacctca
    49021 cctatagtca ttaagctggc tctgtttagt gcattggcag caataggata taaagtatca
    49081 ttaattaaac tggatttacc tgaaccagaa acacctgtaa tacaagtcaa caaacctaaa
    49141 ggaatactga catcaacatt ttgtaaatta ttgcaagaca cgccttttaa atgaatcatc
    49201 cgatcatggt taaccggaat tcgattcttg ggaatttgta tttccttttt cccagacaaa
    49261 tattggccgg tgagagatgc ggggtttttc attacctctt caggagaacc gctggcaact
    49321 acttccccac catgcactcc agccccaggc cctatatcaa gaacaaaatc agcgcttcgg
    49381 atagcatctt catcatgttc cacaacgatt acagtatttc cctggttgcg taaatgcatt
    49441 aaagtactca ataatcggtc gttgtcacgt tggtgcagcc ctattgaagg ctcatccaaa
    49501 atatacatca cccctacaag ccctgaacct atttgactag ccaggcgaat acgttgagct
    49561 tctccgccag atagagtctc cgcgcttcga gccaaagaca aataatccag cccgacattc
    49621 accagaaacc ccagccgctc gacaatttcc ttattgattt ttgctgcaat ttcaccccta
    49681 tatcccgata acttcagctc tttaaaaaac tgataggctt gctcaataga atagctggtg
    49741 atttctggta gatttttatt atcaataaat acatgtcgag cttcttcacg taatctcgct
    49801 ccctgacaag attggcaagg gcgtgaggat aggtatttgg caagttcctc ccttatcata
    49861 ccagaatctg attcacgata gcggcgttgc atgttaggaa ttattccctc aaaactatgc
    49921 cgcttcacca tgtatcctcc atgcggccta tgatactgaa actcgattat ttcacgacca
    49981 ctaccataca aaacgatttg ttgcattgct tcacttaaat cacaaaaagg cgtactggta
    50041 tcaaaaccat agtgcctcgc aagcgactca agcatgctga aataatacgt ggttttccta
    50101 tcccatcccc tgattgcccc ttctgccaga ctagcggttg catcgtggac tactcttgac
    50161 ggatcaaaaa actgatccac tccaagccca tcacatgagg aacacgcacc gacaggattg
    50221 ttaaatgaaa aaagccttgg ctcaagctca cttaaactgt aaccgcattc agagcatgca
    50281 aacctggaag aaaataaaag ttccttgaaa tggccatcca ttgaagaaac gatggcaagg
    50341 ccatctgcca gattgagtgc attttcaaaa gattcactca aacgttgagc caaatcaggg
    50401 cgcactttaa aacgatcaat aactacttca atagtgtgct tggtgcgcaa tcctaattta
    50461 gggggagaat ccagttcata aagctctcca tcaatacgtg cacgtacgta cccctgcgct
    50521 tgcaattgtt gcaaaaactg gacatgctct cccttccgtt cacgaacaac aggcgccaga
    50581 atcatcactt tactgtcttc tggtaaagcc aatacctgat caaccatttg gctaatggtc
    50641 tgagcatgca agctggtgtg gtgggtagga caccttggct ctccgactcg ggcataaagc
    50701 aagcgcaaat agtcataaat ttcggtaatg gtgcctaccg tagatcgggg attatgagag
    50761 gtagcctttt gttctataga aatcgccggg gatagcccct ctatcgaatc aacatcaggt
    50821 ttttccatca tggacaaaaa ttgtcgagca taagcagaca aagattccac gtaacggcgc
    50881 tggccttcgg catagagagt atcaaaagct aaagacgact tcccagaacc agacaatccg
    50941 gttattacga ttaatttatc tcgaggaagc tccaggtcca agttcttgag attatgggtt
    51001 cttgcgccac gaatggtgat ggtatgcata aaattcgcta atttgactta aaaatacatg
    51061 agtatatctc tgctataata ataaaacaaa ttgcacacta tgatgcgaaa tagggatttt
    51121 aatatgaaat cactaataaa aagcttgctt ttcattgtgg ccttctcaat caactcgtct
    51181 gctctctatg ctgccaatag taatacccga gtgattcaat gggctcaaca aacccttata
    51241 gatactttat ctgtcagcta tactacaaaa tcccaagact ttgccactgt tcgcaaaaat
    51301 tactcactta atgcctggag tggaataacc ggatttttag gtggtaacat gcaaagcata
    51361 cgcgaaaaac aactaacgct tcatccggtt ttactgcccg acacccaaat tctatcaaca
    51421 ggcctttatt tgggtatgag ttattggatt atcagacaat ccgtagccat agatgaactc
    51481 aatatcaagc tgacttttac cttaactatc ctggggcgat ctggggcagc gggctatcca
    51541 tacatagttc aaagcttaag tatattgctc aatgcattat aattacgaat taaacaggaa
    51601 tcacctgaaa catggattgt ctccatgacc acatggaatc atcaacataa aaggtatact
    51661 ctcactttaa attctgcttc aatataaaca ggacttacga aaaaccccaa ggtcgaggca
    51721 aaaagttttt ttaatgagag agtttagata cactaaatga ccgaattaaa aaaaattttt
    51781 aacaaagaga ttggggtttt tcataagtcc tgataaaagc aacaactgga ggacacaacc
    51841 atgcctaagt gtatgcattt tattttggct actgcattaa tttttgttac aagtacattt
    51901 tatgccagca atctacaaga aatagaacaa gaagtaagtg attcagtgat tactgccaaa
    51961 attaccgcaa agtttgctga aagcaggcac ttgaatccat taaaaatttc aattgacaca
    52021 gaaaagggaa tcgtgactct aagtggctat gctcctgata aacaggcatt catagatgcc
    52081 ttgcagatcg ccaaagacac gaaaggtgtc aaggcggttc atgccaaaga tcttcacata
    52141 aagaaagtca ataccgcttt cacagatgcc ttcatcacgg cagaagttga aactgctgtt
    52201 ctaaaagcca aaattctgga tgatgaatca ataccattag ttggaatcaa tgccagcaca
    52261 acgaatggcg aggtcacctt gtctgggcat gtaaaaagtg cgggctccat tgcagccatc
    52321 attaaacgcg taaatgagat acctggcgtc aaaaaaatcg ttaccaattt ggaaattcag
    52381 gaaaatgcat gagtccatta acccaattta aattcttttt atggtttttt tgccacttta
    52441 aagtagcatt gattggttat ttaaagccta agttaattca gcttaccgat aatgaaattg
    52501 ttgtgcgact gccattgaca agacgcagcc gcaatcatct gcattccatg tattttggtg
    52561 ccttggcagt tggtgcggat atagctggag gattacatgg tttttatcat gctaaacaag
    52621 cgaaatgtaa agtatccctg gcatttaaat cttttcaagc tcagtttctg cgtagacctg
    52681 aatccgatgt ttattttata tgcacagaag gagaggtagt taaaaaaatg atagaagaat
    52741 caaaaaaatc cggtgaacga attaataaac caattcatat aaaggcttat atcaattatt
    52801 tgacccatcc agaagaaatt gctgatttta tccttgaact ctctctaaaa gttataaaat
    52861 a
//
LOCUS       DAXLDT010000004        52207 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXLDT010000004 DAXLDT010000000
VERSION     DAXLDT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 52207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52207
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.004"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2137
                     /locus_tag="ACF2SP_000181"
     CDS             <1..2137
                     /locus_tag="ACF2SP_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFD2357997.1"
                     /translation="LQFNYWNQLITSQLELSNSFPHVFSGKIRLNPHSTNKNIFSGGI
                     DIGGNRNQIQWGGEFEGPGKITINGSLQQLKQIKQVIKWRDLHWQDNKNNNLKSSQGR
                     IEVSGTIPNLAIDLKSKLSIDQLKNWQMNASIKGKLPWQWNVDATFSQPLDFSSKLDG
                     LYTKFALKMETQNDHHADLLITIAPGHYQMTENNLMPLIQFTGGNIKGSLSQKQFSGQ
                     GVLFLDAYKKVNFNFKFPEFNVYQDIAQQTIDAKLSILFNSLDFLQNASPYLINPKGQ
                     LLASIHAKGTLNKPIIESKFTLNKGSVKIPALGLNITDMGLAVFSKKEGWEATGSIHS
                     SEKKLILKGQGSFNKELGGTVSIEGSNFPIVNNKEFQVNISPQLKVRFTPSLCQITGT
                     IQIPNAHIKPQTFTNSLVATDDIVFTSKNTQPIAPLFNTNMSVRVEMGEEVELTFKGL
                     HAYLAGMVTINQLSQGPITANGELTVKKGEYKAYGQDLSIEQGQLIFTGGRIDNPEIN
                     LRASKNISNSFGSVSGSSELFNFNNYNNLNVGNNFNVGVEVAGRLQSPKIQLFSNPPT
                     LSQADILSFLVIGRPANQADKAKGQLLLTAISSMNLGTGTNGTQLLEQLKQKLGFDFN
                     VQTTTNFNQQTNKISESTGVVVGKSLSERIYLSYNVGLSQTDPNVLTLKYILNKFLSI
                     QVSSSDSGNGVDILYTKSTSSPKKTKPDK"
     gene            2171..3166
                     /gene="hemB"
                     /locus_tag="ACF2SP_000182"
     CDS             2171..3166
                     /gene="hemB"
                     /locus_tag="ACF2SP_000182"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFD2357998.1"
                     /translation="MNEYNLPLRLSRLRKNAMSRALIRETRLHSQQLIAPLFISEYLT
                     EAKEISAMPGQFQLGLNCIPEEIEALSKSGIPAVILFGIPKHKDACGSESFNSNGIIQ
                     QAIKKIKSVNSDMLVITDLCFCEYTDHGHCGILDGNQINIASTLELLGKQAVSHAEAG
                     ADWVAPSGMTDGMVLAIRQALDTASFHEIPILSYSAKYCSCLYGPFREAAQGAPQFGD
                     RKAYQMDPANSEEAIRETRLDLEEGADMIMVKPAGFYLDIIYKLKQHFSEVPLCAYQV
                     SGEYSMIKNAAKNNLIHEEQAMIESLIAIKRSGADFIISYFAKDMAKLLNSQNCI"
     gene            3303..3695
                     /locus_tag="ACF2SP_000183"
     CDS             3303..3695
                     /locus_tag="ACF2SP_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2357999.1"
                     /translation="MNLMLKGLSALAISLGAATTFAAPAYLTTHNRTGEESNAYIAGS
                     IPSLYPTAAYSTNQVYWNLVKLACYGHTTNGQCPALIKMATNTANPIDIGYVTLDLNT
                     GDITPKTLSAKGYTLRAIGPGEAEITKN"
     gene            complement(4036..5472)
                     /locus_tag="ACF2SP_000184"
     CDS             complement(4036..5472)
                     /locus_tag="ACF2SP_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFD2358000.1"
                     /translation="MKIRHKPLRTLVSTAVLGVLATGTLNAGGFSLYTEGAGYTTGNF
                     AAGVAAEAADASTGWYNPAGLVLIRDQQAVFAGVGVFPTAKLSGISTYSTINPPPAPS
                     FIYSEVFNNINGAEDAFVPSFHYALPLGENATFGLSVVSPFGLATDWGPDSPVRYQAT
                     FTELITTNISPEIGGRLTEHVALGAGLDLQYARVKYNRILGAPNIMNALDLNPMLADS
                     LTYNKGHSFGVGFHVGVLGMFNDNHTRLGVNYQSEMRHEFYGYSRLTGPLAIPGNIFA
                     PTFPQLGVFQSDDLFSNAIDLPDVVTISAYHDVNDKFALLGSLVYTAWSSFKTVQLTN
                     VAAPRLAANGGVSQVKAISVSPQNYTDTLRVAIGANYHVNDKLMLRVGGGYDPTPTND
                     IDRDVRLPDTDRWALSAGAHYQATNNIGVDIGYTHLFTSSDPVINRTDVLTATTSYNV
                     TASGTAGANLVGVQATWTIDKEPPAPTK"
     gene            5812..8373
                     /locus_tag="ACF2SP_000185"
     CDS             5812..8373
                     /locus_tag="ACF2SP_000185"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HFD2358001.1"
                     /translation="MQQVVIKFGGTSVSSRATWNNIAAITQKHLNTGVQPVIVCSALT
                     QISNKLEKAIEAALLDEHHSLFNDIQSSHLNLAEQLEVNHQLIANDLHQLQQWLTGIS
                     LLKQAPAKTHAQILSLGELMMTRLGHAFLEKQGIKVKWYDARELLTSTPTLGGETMNY
                     LSARCESEYDPALVEKFLSTGAQAIITQGFFAANPHGETVLLGRGGSDTSAALLAGKL
                     QAASCEIWTDVPGIYTANPHQLPHARLLKQLNYDEAQEIASMGAKVLHPNCIPPVRKA
                     NIPMVVKYTHLPEHSGTLITKDIDESAPLIKSIQVKHSILLISIDTLNMWQQVGFLAD
                     VFAAFKKHGFSVDLLSSSEFNVTLSLDVNAKIHDRPAINALLDDLNQFGRAKLIEPCS
                     AVSLVGHHIRTVLPHLGPALEVFEAKQVYLMSLASNDLNLTFVVDESHADKLCQRLHH
                     LLIESNPQIFYYSKSWHEEFGKPNVRPTPWWEIERDRLLTTSAIHSPCYVYHSPTQAT
                     RARQLSALESIDSLFYAIKANPFPSILKTLEKEGIGFECVSIQELELVLKLFPNIKKE
                     RILFTPNFAPKSEYEFALQTGCYVTIDSLYPLENWPKLFKDREVIVRIDPGTGAGHHK
                     HVSTGGNESKFGITQNDISKILSHTKANHIKVIGLHAHSGSGILSTDLWQQTAVMLAS
                     LTDQFPEVRSINLGGGLGIVEKPGQHPIDFAALDAQLMAVKSQYPGLAIWLEPGRFFV
                     AESGVILAKVTQCKEKGKVKFIGIETGMNSLIRPSLYGAYHEIINLTRLHEEKAGFAH
                     IVGPICESGDTLGYDRLLPVTREGDVILIANTGAYGHCMSSHYNLRPPAQEIVLE"
     gene            8378..11608
                     /locus_tag="ACF2SP_000186"
     CDS             8378..11608
                     /locus_tag="ACF2SP_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFD2358002.1"
                     /translation="MRLIDSEQRSQATDPAESFIVQAPAGSGKTEILTQRFLRLLSTV
                     QSPEQIIALTFTRKAASEMRERIVQALHMAASNQPAVSAHQQSTLNFAKQALQRNKQY
                     QWDLLEQPNRLKIITIDSLCQSINRAIPLLEKQVAYPDISETPDSHYISAARCCIQYA
                     IATPEYQEAIKTLLLHVDNKQERLIQLFQALLSQRDQWLPTLFLAREQEKSTFEQALR
                     LIEQHELSRLRNSLPSDLALELVLLSRELAVIENKPDSPRFILREWYEFQKCDQQIAG
                     ALSKLLLTSDNQLRKSFDHHVGLVKKNCAPNEFTRIKTASSDLFTKLQDCPDFLDALL
                     KVNQLPTPEYDKEQWDVLQALFLLLPLLVGHLQVSFGEHNEVDFTAISQQALAALGDL
                     DNPTDLALYLDHSIHHLLIDEFQDTSITQFELLTKLVQGWLPGEGKTLFLVGDPMQSI
                     YRFRQAEVGLFFQAKEHGIGPVRLKSLELRCNFRSTETIVNWVNDHFSKIFSQQVDIE
                     SGAVSFHPSVHVIEKNESSTIQAVQFKNREQEARHLIEIINSELNRNPEQTIAVLVRT
                     RTQLPEIISLLRQNNIPYQGTDIDLLANLVHLRDVWSLAHALLFPGNRLSWLSILHSP
                     YVGLGLSDIHCIAQYNSRKSIYHNLLHLDKIQDISEEGRIRANFFIQIMNQALLCRSH
                     SRLSDWIADTLKNLHVEKILDKAQLNDLEQLWMLLDQYENEGRIPDMNEFLNEFNKLY
                     AQQTTPSQLQIMTIHKSKGLEFDTVILPGLGAQASRGDSPMLRWLKLPTQHHGNLLLV
                     SPIKSANQDSCPLYDYLDQLDEEKSAYEAQRLLYVAVTRAKSRLYLLDHSAKSYKSSF
                     RNLLKYQEFIADPDEAQHEEQEFPLPKLVKLPIEYYQKQIPSLVQSQESSCPEISSGI
                     PRLIGIVSHQLLQWICDNHPQTIDHVPWNLVNNELKTLGLNQEAQQTALSMIKNQISQ
                     MYENKIGLWILSRHENEQNEYELLVEYENKLVTRIIDRTFIENGTQWIIDFKTGKEDI
                     NTLQQHRQQLNEYGRYLSDCTKFPIRCGLYYLANGHWIDWQYEMSA"
     gene            11816..12889
                     /gene="apbC"
                     /locus_tag="ACF2SP_000187"
     CDS             11816..12889
                     /gene="apbC"
                     /locus_tag="ACF2SP_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFD2358003.1"
                     /translation="MTIEDTVIKLIGSLKDPLLDLTGKEMNLQYKITTSNQAIHVVLT
                     AGYPTSLLETSYKPIVQKIVQDEFPNYQVTISIQQFIKAHKTQLTGKALRGVKNTIAV
                     ASGKGGVGKSTVTVNLAAALAKLGARVGILDADIYGPSIPLMLGETKPVQVKDNCYIP
                     VEAHGMQAMSIGYLTDTNQALIWRGPMLAKSLIQMLDITLWNELDYLFIDLPPGTGDI
                     QLTLVQKIPLTSAIVVTTPQNVATLDAQKAITMFSRTGIDVLGIIENMSTHICSHCGH
                     QEAIFGRGGAAALCDAYQCTLLGQLPLDSHVRRHCDEGVPTATHSSNQLTDTFIKTAM
                     RTAIELSKKPINYADRFPKIVVE"
     gene            13005..13559
                     /locus_tag="ACF2SP_000188"
     CDS             13005..13559
                     /locus_tag="ACF2SP_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HFD2358004.1"
                     /translation="MIRLLIITICVLCSGLTIAKSSYQIDLILFAQPQNGVKSIKSDS
                     PVPLIPVSPNAIQLHSDNNKKHRPYTLLPRSQSKLSDQHYLLSRKSQFQILGHYSWRQ
                     PAKSDSLVALPKINHNGWQIQGTLKVRQSNYYLFDADLQCLSPGNQETSFTVSQKQRL
                     KGSTIYYLDHPQLGMLVKIHKIAS"
     gene            complement(13607..14497)
                     /locus_tag="ACF2SP_000189"
     CDS             complement(13607..14497)
                     /locus_tag="ACF2SP_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2358005.1"
                     /translation="MNLRDLHYFVILADVKHFGEAAKRCFVSQPTLSMQIKKLEEELG
                     VVLFERTNKQVLLTDQGSKLLDRTRKILILIDEMKELARQSEDPFTGELRLGVIPTVS
                     PYMLPLVMPELKNEYPRLKVWLIEDKTHRLITKLEQGELDVAIMALPIDKRFSCQILY
                     EEKFYFACANTHPLAQAKSVNINDLKNQPIMLLEEGHCLREQAMAVCQLAKADDIADF
                     TATSLETLRLMVQAGMGVTLLPALSTLTASTNHLKCIPFSEPAPSRILGLFWRAGTPR
                     QVCFNAVAELITKNVQSKLA"
     gene            14757..15929
                     /locus_tag="ACF2SP_000190"
     CDS             14757..15929
                     /locus_tag="ACF2SP_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358006.1"
                     /translation="MVGVFFLLFQFFLQLSSGIIVGAIMHEEHISALSAGLLSSAFYY
                     VYTSFQIPVGILFDRYNTRYLLSVNAALCAIGCFVFASGHNLPTLFLGRLIIGAGSAF
                     AFIGLSHLLRQHFPLKQYAFMIGLSETLGFTVTVLGMIGMGSVISHFGWRYFINIAGI
                     IGFLIASLCWLCIPNSKPIVSFPHKYKQQIIPILKNKLAWFNGLFVCLEFSVITVFAA
                     MWAVPFLQLKLDCTIKTASILTSMVLLGAGLSCPLLGQISVHLNRRKPLIHASCLSTA
                     LLLILVLYLPTQNKLLIGILMFSMGLTCGAYMLAYSISNELAPPEFLSTCTGFTNTLA
                     MLSAPLLQPLVGFFLDVLSNHKSQYSLADYQLALLIVPFAMLLASLFTCLLPEKSN"
     gene            complement(15931..16881)
                     /locus_tag="ACF2SP_000191"
     CDS             complement(15931..16881)
                     /locus_tag="ACF2SP_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358007.1"
                     /translation="MMPKRIWLLLLSMFLFLSGSVFAVEEPEEDKVVQTNEPTVGSWV
                     FTGMVTNESGERYGYFFQMQRQGTDFYAKTALIDGQTNQLILFYEASEKIDSSTQLNW
                     QVGRSFLRYNPINDSWIFGVKVENAKGFNFKVDMLKQDNKDVETQVLRPGVELQAVQT
                     SRLNGHVQTGVDNKEQFVTGNKAWFSKLWFSKDQNSPHDISTIFCRLSNDNGFYSANL
                     KEPDATGAAVAGWRDPSGNNVKMSQFVSIKTLTDNQCLLSVGLPRLKLKLVNTIKQED
                     DFPLTVAGFSEENPKGFCFISKQTFKQIKESAVQAVTNLG"
     gene            complement(16883..17854)
                     /gene="lpxK"
                     /locus_tag="ACF2SP_000192"
     CDS             complement(16883..17854)
                     /gene="lpxK"
                     /locus_tag="ACF2SP_000192"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFD2358008.1"
                     /translation="MSFFVNRIWYGNHFLQWMLVPFSWLYRIVIRTRRWYLQRFCQQL
                     YPIPIIVVGNVTVGGVGKTPLVIEIAKKIQQKGLKVGIVSRGYKAAIKHFPYEVKLND
                     SAELVGDEPLMMARKINCPVVIAPKRNEAVRYLLDKHSVEIIISDDGLQHYKMGRSIE
                     IVVIDGMRKLGNGFCLPAGPLREPDSRLKQVDFVIVNQGAAEGTYSMELIPKNIVRLS
                     TQEEVSNDLFTSEVAAVAGIGNPQRFYSTLSQLGIKFNPYSYPDHHQFKPHDLNDIDL
                     PVIMTEKDAVKCYSFSSDKLYYLPVEAKLNDSFWEAFWSHQQLQGYY"
     gene            complement(17854..19620)
                     /gene="msbA"
                     /locus_tag="ACF2SP_000193"
     CDS             complement(17854..19620)
                     /gene="msbA"
                     /locus_tag="ACF2SP_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFD2358009.1"
                     /translation="MKNNLPIKSRLLYKRLLSYVKPFWPVLLLGVLANILYSGIDAGF
                     TYMTKLFLDKSFITIDLDFVKQIPLIVLIGITLRGLVSSLGSYCMTWVARSVVKVLRQ
                     TVFSHIIHLPADYYDEATSGQLLSKILYDVEQVAQVSADALTDFIQNICLVIGLLTVM
                     MVICWQLSLMFLLTIPFVGIIVNYTNKRVRRISHKVQKTMGEVTEIASEAIEGYRVVR
                     IFGGERYEITKFNKATEYSRKNDMKVAISKAINVSGVQLVIAIGIAMIIMAAIHLSTV
                     ITISAGSFLAIIAAMLQLIKPMKTLTTLNATIQRGLAGAESVFNLLDLPLERNNGLIL
                     KEKVRGEIEFKHVYHAYRQSQNILHDVNFVIEAGTSVALVGHSGSGKTTIASLLPRFY
                     ELSQGMITLDGMPIQQLSLESLRKQISLVSQNVTLFNDTLANNIAYGRFDASREQIIT
                     AAKLAYADEFIKQLPDGYDTRVGENGVLLSGGQRQRIAIARAILKDAPILILDEATSA
                     LDSESEHYIQAALEQVMKGRTTLIIAHRLSTIKHAHKIIVLQHGRIVEQGSHQELLDM
                     DGHYAQLYKVQQFGRINEEVVV"
     gene            complement(19707..20489)
                     /locus_tag="ACF2SP_000194"
     CDS             complement(19707..20489)
                     /locus_tag="ACF2SP_000194"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358010.1"
                     /translation="MYITKELAVRMEGCIKQSHIEVTKRYSQGKILEINGGAACFSGF
                     ESFLSQVVGWGFKTEPNQYKAEIEAIENFYKSLGHNRVDIELCPFVGNHLINFLSQRG
                     YYITELNNVSVLDLKSYHLVAHSKDDGFIIREVKQSELEEWAKRVAIGFGFLEAQEQF
                     YQYVSAKGVLAFAAYDHGNLVAGATIAIHGDACDLGVTSTLPLYRGKGLQKKLLFARL
                     NLAKQYGLEFATVTTQPGTVSDLNVQKAGFRCAYTRIKLTME"
     gene            complement(20510..21511)
                     /locus_tag="ACF2SP_000195"
     CDS             complement(20510..21511)
                     /locus_tag="ACF2SP_000195"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFD2358011.1"
                     /translation="MYSLLRPLLFRLDAEKAHSLTLSLLHYLPGFYFRKIAGQPVHAM
                     GLVFPHQVGLAAGLDKNGEHLDALAKLGFSFIELGTVTPKGQAGNPKPRLFRIAEANA
                     IINRMGFNNSGVDVLVENVKRANYKGILGINIGKNKETNLNQAAEDYLYCFRKVYDHA
                     SYVTINISSPNTPDLRQLQQGDYFAELLTQLQKEQIKLADQYGRHVPLVVKVSPDETD
                     ETLKQMTDIILQYGIEGIIATNTTCSREMVKNLPCSEEQGGLSGRPLMELSTRCLRLL
                     KQYVGNDVTLIGVGGIDSLESAKDKINAGASLLQVYSGLVYKGPELIHDIVSGLNAI"
     gene            complement(21797..22795)
                     /locus_tag="ACF2SP_000196"
     CDS             complement(21797..22795)
                     /locus_tag="ACF2SP_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358012.1"
                     /translation="MAVSNKKNSEFTPSLRSHMIAALLSMGAAAIILWLASGMTLAAV
                     IVDSFSLGFYIGFMLLMSAAPAVLASPVILPGLLLVVSGLFLGAVAATFAHRLKNNPS
                     LPQNNNGNGLINELTKDKSELLISHDDLQCQSNSLEGENTLSQQNEDNVTGEKFGFMK
                     FWGSLGLTSFASSGATALLGWLATDFVVSLSIIPVFLLGMTLAIVLVPLSIGLSGIFA
                     LPAVTALMFALGGFATGVFAATLAAYFIADNQAQDHPVEPIIRSSMERQCKDQGSHQK
                     ILNGLKKDKLSANEEVINSDLNEQKSRGPKTSKSDTGVKEEHINTAEEYSFCGLRF"
     gene            complement(22844..23530)
                     /locus_tag="ACF2SP_000197"
     CDS             complement(22844..23530)
                     /locus_tag="ACF2SP_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358013.1"
                     /translation="MRKLVLWGHGIEDYREMFDLPQGVENLRLLEYGCGPSAVNYQQT
                     QVKKKGVVSCDPLFVLDKDTLLAKAKMIFANMAEEVRQHQDQFDFSRNGSLDKLLQER
                     QEGMKQFFADYEQGKADGRYLGLTDYHLPFADFTFDFALSSHYFFADLDDQTVDFHLI
                     AIRELARVAKEVRIFPLIDREGNTSQFLGPVLLGLQQENYGVEVREAAFHLHKSGNAM
                     LRVWAQQCVV"
     gene            23865..25025
                     /locus_tag="ACF2SP_000198"
     CDS             23865..25025
                     /locus_tag="ACF2SP_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFD2358014.1"
                     /translation="MYTGLDFQLGETYDMLRDSVNQFAQTEIAPLAAQIDEKNTFPNH
                     LWRKLGEMGLLGITVSEEYGGANMGYLAHAIAMEEISRASASVGLSYGAHSNLCVNQI
                     FLNGNNEQKQKYLPKLISGEYIGALAMSESNSGSDVVSMQLQARSAGDKYILDGTKMW
                     ITNGPDADVLVVYAKTDKQAGSKGITAFIIEKGMPGFKTAQKLDKLGMRGSNTCELVF
                     DQCEVPSENVLGTVNQGVKVLMSGLDYERTILAAGPVGIMQACMDVVLPYVHERKQFN
                     QAIGEFQFIQGKLADMYTDLNACRAYLYAVAKACDNGRVSRKDAAGVILYTAEKATQM
                     ALQSIQILGGNGYINEYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFKETA"
     gene            25037..26221
                     /locus_tag="ACF2SP_000199"
     CDS             25037..26221
                     /locus_tag="ACF2SP_000199"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFD2358015.1"
                     /translation="MEHNDVVIVAAKRTPMGAMLGNLSALSSPELGAVAHMAALSQSG
                     IAPAEIDEVISGCVLQAGIGQAPARQAAIKAGIPNSTGATTINKMCGSGMKAVMLAHD
                     LIKAGTANVILASGMESMSNAPYLLSKARAGYRLGHGELKDHMFLDGLEDAYDKGQLM
                     GCFAETTAKHFNFSREQQDEYALRSMTKALKAIENGAFREEIAPVTLTTRKGDITVDV
                     DEGPETAKLAKIPQLKPAFQADGTVTAANSSSISDGAASLILMSASNAEKRGIKPLAK
                     IIAHASHSQAPQWFTTAPVDAIRKVMNKASWKQDDVDLFEINEAFAVVAMAAITQLEL
                     NPEQVNIHGGACALGHPIGASGARILVTLMHALKHQGKKRGIASLCIGGGEATAMAIE
                     LI"
     gene            26359..26673
                     /locus_tag="ACF2SP_000200"
     CDS             26359..26673
                     /locus_tag="ACF2SP_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358016.1"
                     /translation="MLRHSLIYLLLSILIVVFAKYAHLIVVYIDMFFTYVNLKLTPIF
                     SQTGWGLIIRKILVLMLLPIIIAGIPALIYRALKGKDMPHFIAIVWIIWTIIVLSDIL
                     IR"
     gene            26683..28290
                     /locus_tag="ACF2SP_000201"
     CDS             26683..28290
                     /locus_tag="ACF2SP_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HFD2358017.1"
                     /translation="MAKLTTQINTSSQEFKNNQANMQALVTDLREKIHQISLGGDEKA
                     RTKHQQQGKLLPRERLHQLLDPGSPFLELSQLAAYQVYEDTIPAAGIITGIGRVAGNE
                     CVIVVNDATVKGGTYYPLTVKKHLRAQEIALINHLPCIYLVDSGGAFLPLQDQVFADK
                     EHFGRVFYNQAQMSALNIPQIAVVMGSCTAGGAYVPAMADESIMVKNQATIFLGGPPL
                     VKAATGEVISAEELGGAEVHCRHSGVSDHYAENDAHALYLARVAISNLNRKKPDSIHR
                     VDTVPPLYDSEDLTGIIPTDPRKPFDIREIIARVVDGSEFDEFKALFGTTLVCGFARL
                     YGYPIGIIANNGILFSESAQKGSHFIELCCQRKIPLVFLQNITGFMVGSKYEASGIAK
                     HGAKMVTAVANANVPKFTIIVGGSFGAGNYAMCGRAYAPRFLWAWPNARISVMGGEQA
                     ANVLAQITREKYAKQGKEWSLEEEEQFKTQMRSQYETQGNPYYASARLWDDGVIAPQD
                     TRKILGLGLSAALNAPIEDTRFGVFRM"
     gene            28310..29104
                     /locus_tag="ACF2SP_000202"
     CDS             28310..29104
                     /locus_tag="ACF2SP_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFD2358018.1"
                     /translation="MSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAIND
                     TNVRVIVLKANGKHFSAGADLTWMQSMANFTEEENLEDSLVLGNLMYSLSQSPKPTIA
                     MVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKML
                     FMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVAN
                     KKIDEELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV"
     gene            29097..31061
                     /locus_tag="ACF2SP_000203"
     CDS             29097..31061
                     /locus_tag="ACF2SP_000203"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFD2358019.1"
                     /translation="MFNKILIANRGEIACRIIKTAHSMGIQAIAVYSAADRNSLHVRL
                     ADSAYYIGEAPAKESYLNIDHIIQAAKESGAQAIHPGYGFLSENPDFAKACEQAGIVF
                     IGPSIKAMEAMASKQLAKQLLEKTKVPLTPGYHGGEQSEEKLLSEAKKIGFPVLIKAA
                     NGGGGKGMRAVHDEKEFHDALAGAKRESMASFADDTMIIERLVLNPRHVEVQIMADNH
                     GNVVHLFERDCSIQRRHQKIIEEAPAPNLSPVLRQRLAEAACEVARSINYRGAGTVEF
                     LVDGEDKFYFMEMNTRLQVEHPVTEMITGLDLVAWQIRIAANDTLPLLQNQIQAQGHA
                     IECRIYAEDPYQGFIPSIGQLQFLKEPSGDGIRIDTGVTLSSEITRYYDPMIAKLIAW
                     GHNREEALQRLERSLSHYDIGGVKTNIPFLRAICRHAKFKEAKLSTDFLEKENINLPK
                     PDNELGILLAISCDYLGMVNRTMDPLLQDAFGWQMHLSSHWVWRYQLNSTIIETQITP
                     IDNKKFKAKIENKEMVIHARYDIDQLIIEIDQKSVKARVENKDHHLIFYTDKGQLSIE
                     RFYWSKLDAQTSAHKGQLTAPMPATVVAILKNIGEQVKAGESLIVLEAMKMEHTIHAP
                     IDGILSDIFYSVGSQVSEGAELLALSESDT"
     gene            31072..31980
                     /locus_tag="ACF2SP_000204"
     CDS             31072..31980
                     /locus_tag="ACF2SP_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFD2358020.1"
                     /translation="MDYPQHVTIIEVGPRDGLQNEPSFLHSDKKIELINLLSQTGLKE
                     IEVTSFVSAKAIPQLADNEEVFQSINKTPSINYSALVPNERGMLKALEMGVQNIAVFT
                     AASELFNQRNINCSIKESIERFKPVLALAKTNQIRVRGYISCVLGCPYEGYIQPSQVV
                     SVTKMLLDLGVHEISLGDTIGVGTPRQTQLLLDAILPILPITQLAMHFHDTYGQAVAN
                     IYASLEYGVNRFDSSVAGLGGCPYARGASGNVATEDVLYLMHGLGIDTGIDIFKIVAA
                     GDMICKALGRKNQSKVANAMLANPCN"
     gene            32137..34077
                     /locus_tag="ACF2SP_000205"
     CDS             32137..34077
                     /locus_tag="ACF2SP_000205"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HFD2358021.1"
                     /translation="MNDKVWTPKNPEQTRMWQFMDFAAKRHCQVFENYQDLHTWSVKH
                     PESFWETLCLFFKLNFDTPPHQILNYYSEMIEARWFSGARFNFAEKLLTRRDNHPALI
                     SLDENDSKSVISYAELYEKVAQCAAGLKAVGVATGDRVAAVMPNTSYTIIAMLAATSL
                     GAIWSSCSPDFGTQAALDRIGQIEPKVLFICDGHQYQGKKHSSSEKVVQLTAAITSLR
                     QVVICPNINEEINLEQLSNASYWNDFIKPEKECDFISLPFDHPVYILFSSGTTGKPKC
                     IVHGAGGTLIQHLKELGLHSDIKSTDNLCFYTTCGWMMWNWTVSALALGATITLYEGS
                     PTYPTDNRMFRLIDEEKVTVFGTSAKFISAIEKAGVKPKSEFQLSELRCILSTGSPLL
                     PKNYDFVYEHIKKDLQLSSISGGTDIISCFALGNPILPVYRGELQCIGLGMEVAVYDE
                     QGHSIKQKRGELVCTKPFPSMPVCFWNDKDRQAYKHAYFERFPGVWAHGDFAEITAHN
                     GLIIHGRSDAILNPGGVRIGTAEIYRQVEKVDDVVDSIVIGQDWQDDVRVVLFVKLRE
                     GKKLDDELINLIKTTIRKNASPRHVPAKILQVPDIPKTLSGKVVELAVRQVVHGQSVS
                     NLQSLANPQALEYFKDRDELKS"
     gene            complement(34237..34653)
                     /locus_tag="ACF2SP_000206"
     CDS             complement(34237..34653)
                     /locus_tag="ACF2SP_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK family protein"
                     /protein_id="HFD2358022.1"
                     /translation="MKKMFLAVFTLCSFTAQAAVELKTFQNIVDAIAEGKRITFVISL
                     KKCTSEMPLSSAIVSVTPNAVMVVGDNRVTASDRHFTLDDPVARGTPMFDYSKFNLDS
                     EGDASIKTTVLNASSYERLGSYQMNCKLGDGFKVFG"
     gene            35106..36131
                     /locus_tag="ACF2SP_000207"
     CDS             35106..36131
                     /locus_tag="ACF2SP_000207"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFD2358023.1"
                     /translation="MIELCGLKKSFSGKLALNDINLFIQEGEIFGVIGKSGAGKSTLL
                     RCINILEKPDEGEVIIDGQNLMSLSRKNLALARHKIAMIFQHFNLLNSKTVFDNIALP
                     MRIQGIDEELIKQKIEELLPVVELTDKKDAFPSQLSGGQKQRVAIARALSCSPKVLLC
                     DEATSALDPATTDAILSLLKKINELYGITIVLITHEMDVVKRICQRLSVMVDGKIVET
                     TALSNIFNKPESLARKMLYAQISPELPTCLTRRLADYATEKPLVRLFFQGEEATVPFI
                     SQTSRELNMDINILLANIDRFDGVTCGVLVVELTANPLLLEAFINRCEQAGITVEVLG
                     YVLPDGL"
     gene            36109..36756
                     /locus_tag="ACF2SP_000208"
     CDS             36109..36756
                     /locus_tag="ACF2SP_000208"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFD2358024.1"
                     /translation="MSYQMVYDLLSATGETLYMVFFSTLFAVLLGLPLGILLYSSSRI
                     KPNIKLNKILSALINIFRSIPFIILLVAIIPFTRLIVGTSIGINAAIVPLTVGATPFF
                     ARLVDNVLQSLPPGLIETGYSMGANTRQIILHIILPEAQSGLIHSITVTAITLINYSA
                     MAGAVGAGGLGTLAINYGYQRFNAGIMIATVIVLIILVQLIQMVGDYLAKRCTHY"
     gene            36769..37548
                     /locus_tag="ACF2SP_000209"
     CDS             36769..37548
                     /locus_tag="ACF2SP_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFD2358025.1"
                     /translation="MRILSCLVLIISLVACSSKPAPNTLVIGTIAGPETELIETAKQV
                     AEKEYGLSIKIVEFNDYNLPNEALQDGSLDANVYQHLPYLKAAILSHGYDLQAIGRTF
                     VYPMGIYSKKYKTLSELPENGIIAVPNDPSNEMRAFLLLEKAHLITLKNTTNSGIQDI
                     ESNPKQFKFKEIDAAQLPRVLPDVDAAVINTTFALPAGLSPSKDALFTEGKDSPYANI
                     IVIRRDTEKRPQLELFVKALNSEEVKEKAKKLFGEDAIPAW"
     gene            37628..39046
                     /gene="ceg25"
                     /locus_tag="ACF2SP_000210"
     CDS             37628..39046
                     /gene="ceg25"
                     /locus_tag="ACF2SP_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg25"
                     /protein_id="HFD2358026.1"
                     /translation="MAFYNSIPLIGKHLKSESRAKKYTSEEFKRLEREYRDHIGLPEN
                     SSFSSDAFDKLVSSRVDDFRAFPKTNLPGGLLFHDKLKEANTTEGENIRDVLSKTVLA
                     PTEKFKTAQKNFTESTSTFKELTKLIPSKFRAQDLVGTMKELTDDARKAIIEQQAHEI
                     QMLKEKLDPQKEGQAGNYVSDFKKALGLKDNDEVKKVTEGLIKELEETHKKQLGEFDK
                     STSDSLNQLHKASASESSEYLFLANLYENNKEMRQTLEKLYAKERQRKGLPPESTTVQ
                     VGISENKIDLSGIKLKDIPVIKSITGREIHQQPDGSFAIQMPMINPFYYQDPRQNVKT
                     DMMLIAQAIKASGYDSIEMNCNFPNSEKVAMERGRQAFAACIEAGFPVDKITINVNGK
                     PMKADELFKEHPKTYQDIMSRASKISKDYEDIKAPKTSTAPVNTVAVKQEIATLRAKQ
                     QQEERAQTQPVDEQRHGMTPNN"
     gene            39372..39539
                     /locus_tag="ACF2SP_000211"
     CDS             39372..39539
                     /locus_tag="ACF2SP_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HFD2358027.1"
                     /translation="MSTRRFDEFDNEMDDFSDAEPVITEASPSTKLERRRKIEDLLEE
                     KRLREELEEFG"
     gene            complement(39536..40267)
                     /locus_tag="ACF2SP_000212"
     CDS             complement(39536..40267)
                     /locus_tag="ACF2SP_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2358028.1"
                     /translation="MNRIKAVGYENNELKEKAQLLADQLNLQLDQNADTCLFVTPEKL
                     TLKIRNFSLMFADFSAMTWSKRRGEGKKQGLIRACKPTKGIKIFDATAGWGKDAAILA
                     TFGADVLMLERHPVMAALLADALSRRNEADIQKMCLSLIASDAISFLHSLQEKDYPDI
                     IYIDPMHPERNKSALVKKEMQVLQQLIGTDYDAMELIKLSLSHVKSRVVVKWPQKVKP
                     LLPPDASIDGKTVRFDIYMPQFSSN"
     gene            complement(40270..40800)
                     /gene="gmhB"
                     /locus_tag="ACF2SP_000213"
     CDS             complement(40270..40800)
                     /gene="gmhB"
                     /locus_tag="ACF2SP_000213"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFD2358029.1"
                     /translation="MNRIILLDRDGVINTDSINYIKNVDELIIIPDSITAIARLSAAG
                     YKIGVATNQSGVSRGLYSEDDLSAIHCKLREVLAEAGGQIDALEYCIHLPEDNCFCRK
                     PQPGMLKALAEKLNCSLDSVPFVGDRVSDIQAALAVGAQPIIVLSNMTDMSELAQYPK
                     VPIYNSLLQYVNQLLL"
     gene            complement(40806..42872)
                     /gene="glyS"
                     /locus_tag="ACF2SP_000214"
     CDS             complement(40806..42872)
                     /gene="glyS"
                     /locus_tag="ACF2SP_000214"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFD2358030.1"
                     /translation="MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEV
                     KRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAYDSEGNPTPALLGFAKSCGVTVD
                     ALTKTQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
                     HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFA
                     TRRQAIIHQVQELAAPLGASVVMPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALI
                     ASMQSHQKCFALKNKHGELLPYFITVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQ
                     DKKQPLSAHIPSTEQVIFQVKLGSMYDKVQRIKAMMDHLSQSLNLSSHLAERATLLSK
                     CDLMTGMVGEFPELQGIMGYYYALNDGEETVVATALNEQYMPRFSGDDLPSTDLGKAL
                     SLADRIDTLAGIFAIGQKPTGVKDPFKLRRHALAVVRLLISISAPLNLSTLIKEALAN
                     YGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLDKRLFAL
                     KSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKT
                     VETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVEDESLKQNRLALLQRLQELLQGV
                     ADISML"
     gene            complement(42862..43767)
                     /gene="glyQ"
                     /locus_tag="ACF2SP_000215"
     CDS             complement(42862..43767)
                     /gene="glyQ"
                     /locus_tag="ACF2SP_000215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFD2358031.1"
                     /translation="MTKPTTFQEIILTLQKYWSDLGCVILQPLDLEVGAGTFHPATFL
                     RAIGPEPWSAAYVQPSRRPTDGRYGENPNRVQHYYQFQVVLKPSPLDIQDRYLDSLRA
                     LGVDPLLDDIRFVEDNWESPTLGAWGLGWEVWQNGMEVSQFTYFQQVGGLVCKPITGE
                     LTYGLERIAMFVLGVDNLFDLPWCKTANGIVTYGDVFHQNEVEMSAYNFEHANVEELF
                     KQFDYYEGEAQKLIALQLPLPAYEMVIKASHTFNLLDARKAISVTERQRYILRVRHLS
                     RAVAQAYYQAREALGFPMQKVCNGQ"
     gene            complement(44002..44787)
                     /locus_tag="ACF2SP_000216"
     CDS             complement(44002..44787)
                     /locus_tag="ACF2SP_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFD2358032.1"
                     /translation="MKFTSLLTAGALASTLVSPAIMAADTASASLSDAQKKEIEKVIH
                     DYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLV
                     EFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQ
                     AMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLM
                     GTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN"
     gene            complement(44995..47160)
                     /gene="uvrD"
                     /locus_tag="ACF2SP_000217"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(44995..47160)
                     /gene="uvrD"
                     /locus_tag="ACF2SP_000217"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFD2358033.1"
                     /translation="MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIA
                     WLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHY
                     KEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
                     ALHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAIL
                     VDEFQDTNTIQYAWIRLLAGDHTAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQI
                     IRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILVYSAFNELDEARFVT
                     ERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRVGIAYRIYGGVRFFDRAEIKDTL
                     AYLRLLVNPNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWDASKGILQSTGL
                     GQRGSLALAKFIDLIEKLQVVVANKELDEQISDVIQHSGLYAHFSKIKGDKSESRVDN
                     LQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTLHAAKGL
                     EFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGR
                     EEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFG
                     QGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTECL"
     gene            47381..48340
                     /gene="pip"
                     /locus_tag="ACF2SP_000218"
     CDS             47381..48340
                     /gene="pip"
                     /locus_tag="ACF2SP_000218"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFD2358034.1"
                     /translation="MHTLYPAIKPYTQHELKVSKLHTLYLEETGNPEGIPVIVLHHGP
                     GGEADPHLRRFFDPQRYRIILFDQRGCGRSIPHLEISENNTQLLMEDIDSIRDFLGLR
                     EFVLFGGGWGSLLALLYAQKFPQQVKALLLHQIFLGRQKDIDWFYKHGASLVYPDYWE
                     EFTNTVPENKLDNIPKYYAECLQGSNELARMAAAKNWALWQARCSSLQPHLYVIDQYS
                     DPHFALALATLESYYIINHYFIEENQVIANAHKIRHIPTYIVHGRFDLVSPLSSAWEL
                     HQAIPASNLRIVRDAGHSDRESGIIDALIYASKDISKQGLDAC"
     gene            48334..48771
                     /gene="dtd"
                     /locus_tag="ACF2SP_000219"
     CDS             48334..48771
                     /gene="dtd"
                     /locus_tag="ACF2SP_000219"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFD2358035.1"
                     /translation="MLTVLQRVKEARVDIDGQTVGKINHGLLILCGFEPKDSLENIKR
                     MLDKCINYRIFEDPSGKMNLSLKDVNGGLLLVPQFTLMADTQKGLRPSFSNAASPELG
                     RELFDNLLTLAQKCHQNTQSGCFGANMQVYLCNDGPVTFLLQF"
     gene            48989..49771
                     /locus_tag="ACF2SP_000220"
     CDS             48989..49771
                     /locus_tag="ACF2SP_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFD2358036.1"
                     /translation="MRLILMFASIMGSVILASCASKGSNPIDPFEPFNRKVHNFNMAF
                     DATVLRPPAKLYQAVVPKLVRKGVNNAFNNLDLFPTIASDILQAEGKWVIKDSWRLFI
                     NSTIGVAGLFDVAEKFGLPPHYNDLGLTLAKWGDKKSPYLVIPFLGPSTLRDGAGWLF
                     QFALWSPYVYIDDAGLVYGLIGLRYIDLRTQFFDSEPLLNEALDKYTFIRDAYLQHRN
                     YLIAGTEQDTGTSFVEDKKANKTAMQESTASNNQLGSDYVDE"
     gene            complement(49880..50839)
                     /gene="gshB"
                     /locus_tag="ACF2SP_000221"
     CDS             complement(49880..50839)
                     /gene="gshB"
                     /locus_tag="ACF2SP_000221"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFD2358037.1"
                     /translation="MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTQNDLYC
                     CDGSAYARVHEIHIGDIESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYAL
                     DLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
                     PLEGMGGNSIFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGE
                     PVPFALARIPAKGDLRGNLAAGARGEVVAITERDKWICEQIAPALREKGLYFVGIDVI
                     GDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK"
     gene            complement(50839..52134)
                     /gene="gshA"
                     /locus_tag="ACF2SP_000222"
     CDS             complement(50839..52134)
                     /gene="gshA"
                     /locus_tag="ACF2SP_000222"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFD2358038.1"
                     /translation="MNKHEIPVPHLTTAHSGPLYHVEKIILNKLAEIESWFRKKWQET
                     PAPLTSSVDLRHAGFKLAPVDTNLFPAGFNNLNPEFLPLCIQAAQSALIEYIPDCTKI
                     LLLPESHTRNKYYLQSLNVLKTIFVKAGFIVRIGSLDPDIKEPTEIILEQGETLLIEP
                     LQRQGDKVGLDNFSPCLLLLNNDLSSGVPEILQDVQQRIRPTAKLGWASRLKSSHFQF
                     FEEVAIEFAELVGIDPWLINPYFSAIEGVDFMAQEGIEILAQEVDKILSLINDKYTTY
                     GIENKPFAVVKADNGTYGMSVMMVHNGDEIRQLNRKQRTRMSTSKGSRKVDRVIIQEG
                     VYTFETMPDGSVAEPVVYMIGQFVVGGFYRVHKGRGIDENLNAPGMHFEPLAFAQACN
                     MPCDDLKVVDCPNRFYVYGVIARLAALAAAREIAAIGGE"
BASE COUNT        16566 a        10685 c         9614 g        15342 t
ORIGIN      
        1 tctgcaattt aattattgga atcaattgat tactagccaa ttagaactta gcaactcatt
       61 tcctcatgta ttttcaggaa aaattcgttt aaacccacat tctacaaata agaatatatt
      121 ctcaggaggg atagacattg gcggtaatcg gaatcaaatt caatggggag gagaatttga
      181 aggtcctgga aaaataacaa ttaacggcag tcttcaacag ttaaaacaaa taaaacaagt
      241 cataaaatgg cgcgacttac attggcaaga caataaaaat aacaatttaa aaagctcgca
      301 aggaagaatt gaagtatcgg gaacaattcc aaatttagct atcgacttaa aatccaaact
      361 tagcatagat caattaaaaa attggcaaat gaacgcttcg attaagggca agctgccttg
      421 gcaatggaat gtcgatgcta cgttctctca accattagat ttctcatcaa aacttgatgg
      481 tttatataca aaatttgctt tgaaaatgga aactcaaaat gaccatcatg ccgaccttct
      541 gatcacgatt gctccaggcc attatcaaat gacagaaaac aatctgatgc cattaattca
      601 gtttactggc ggcaatatta aaggctctct ttcccaaaaa caattcagtg gacaaggagt
      661 tctctttctg gatgcatata aaaaagtgaa ttttaatttt aaattcccgg agtttaatgt
      721 ctaccaagat atagcacaac aaacaattga tgcgaaacta tccattcttt ttaactctct
      781 ggattttttg caaaatgcca gtccttattt aataaatccc aagggacaac ttctcgcctc
      841 cattcatgca aaaggaactt taaacaaacc aataattgaa agtaaattca ctttaaacaa
      901 aggctcagtc aagatccctg ctttaggtct caatatcact gatatgggat tagctgtttt
      961 tagcaaaaaa gaggggtggg aagcaacggg ctccattcat tcatctgaaa aaaaactaat
     1021 attaaaagga caaggtagtt tcaataaaga gcttggtggg actgtttcaa tagaaggttc
     1081 aaattttccc attgtaaata ataaggaatt tcaggttaac atatccccac agttaaaagt
     1141 gcgctttact ccatcactat gtcaaatcac aggaaccatc caaattccta atgcccatat
     1201 aaaaccacaa acatttacca atagtcttgt ggctactgat gatattgtat ttacaagcaa
     1261 gaatacccaa cctatagccc ctttatttaa taccaatatg tcagtacgtg ttgaaatggg
     1321 agaagaggtt gagctgacct ttaaggggtt acatgcttat ttggcgggta tggttacaat
     1381 caatcaattg tcacaagggc ccataacagc aaatggtgaa ttaacggtca aaaaaggaga
     1441 atataaagcc tatggtcagg atttatctat tgaacaagga caattaattt ttacaggcgg
     1501 tagaattgat aacccagaaa ttaatttaag agcatcgaag aatatatcca attcatttgg
     1561 tagtgtatca ggatcctctg agctgtttaa ttttaataat tataataacc tcaatgttgg
     1621 caacaatttt aatgttgggg tcgaagtagc tggacgatta caatcaccta aaattcaatt
     1681 attctctaac cctccaacac tttcccaagc agatatccta tcttttcttg taataggccg
     1741 cccagccaat caagcggaca aagcaaaggg ccaattactt ttaacagcta tttcctctat
     1801 gaatttaggt acaggaacta atggaaccca attgctggag caattaaaac aaaaattagg
     1861 attcgatttt aacgtacaaa cgacaaccaa ttttaatcag caaaccaata aaatcagtga
     1921 atcaaccggt gttgtggttg gaaaatctct ttcagagcga atttatttaa gttataacgt
     1981 cggtttatct caaaccgatc ctaacgtatt aaccttaaaa tatatattaa ataaattttt
     2041 aagtatccag gtaagcagca gtgattcggg taacggcgtt gatattctat atactaaaag
     2101 cacgtcatcg ccaaaaaaaa caaaacctga caaataaaac cttaatccat attgtctaag
     2161 gatgtaataa atgaatgaat ataatttacc tttgaggtta tctcgtttaa gaaaaaacgc
     2221 catgtcaaga gcattaataa gagagacgag gttacatagc caacaactga ttgctccatt
     2281 gtttattagt gaatatttaa ctgaagcaaa agaaatcagc gctatgccag gccaatttca
     2341 acttggcctc aattgtatac ctgaggaaat tgaagcttta tcaaaatcag gtatccctgc
     2401 agtcatccta ttcggtatcc ctaagcataa ggatgcttgc ggcagtgaat cattcaatag
     2461 caatggaatc attcaacagg ctattaaaaa aatcaaatct gttaatagtg acatgttagt
     2521 gatcactgat ctttgctttt gcgaatacac cgatcatggt cattgcggga ttctggatgg
     2581 taatcaaatt aatatcgctt ctaccttaga gttgttagga aaacaagcgg taagtcatgc
     2641 tgaagcaggt gctgactggg ttgctcccag cggtatgaca gacggaatgg ttctggctat
     2701 acgccaagca ctggatactg cttcatttca tgaaattcct attttaagtt acagtgccaa
     2761 atattgctcc tgcctttatg ggccttttag agaagcggca cagggtgcgc cacagtttgg
     2821 cgatagaaaa gcgtatcaaa tggatccggc aaatagtgag gaagctataa gagaaaccag
     2881 gctagatttg gaagaaggcg ctgacatgat tatggttaaa ccagctggtt tttatcttga
     2941 tattatttat aaactcaaac aacatttttc tgaagtacca ctttgtgcct atcaggttag
     3001 cggtgaatat tcgatgataa aaaatgcagc aaaaaataat ctaatccatg aagaacaggc
     3061 aatgattgaa agcctcattg ccatcaaacg ttctggtgct gattttatta tatcttattt
     3121 tgctaaagat atggctaagt tattaaactc acaaaattgt atttaattat gagtcaaatc
     3181 aatcaaatca agaactataa aacggctata taaagagaaa ggcttgcctc atatgccaca
     3241 aatcccatat agtaaatggg attggcttgg cccaatcaac tacaatctag aaggagaaat
     3301 caatgaactt aatgcttaaa ggcttatcag ccttagcaat tagtctaggt gccgcaacaa
     3361 cttttgcagc tccagcctat ttaacaaccc ataacagaac tggtgaagaa tccaatgcct
     3421 atatagcagg cagcatccct tcgctttacc caacagcggc ttactctacc aatcaagtgt
     3481 attggaattt agtgaaattg gcttgctatg gacatacgac taatggccaa tgccctgcct
     3541 taatcaaaat ggcaaccaat acggccaacc caattgatat cggatacgtt accctggatc
     3601 tgaatactgg tgacattact ccgaaaactt tatcggctaa aggctataca ctgagagcaa
     3661 taggacctgg agaagctgaa attactaaaa attagttaat tttctccatt tattttgaat
     3721 tcgttatgta ggttgaatgc taatacaacc tacataatca atacaatgca aataaatgcg
     3781 ttcatcttaa ttaaaattaa ttatctctgc ccacgattat ctaaacgtta tttatatcga
     3841 tgcacatacc actaacgcag atgcttacga aaaaacccaa aatccagggg ttgaatgcag
     3901 aaaatgcttt taatgccaga gattagacga gctaaataac acaaataaaa atacttttag
     3961 atttaagttc tgactaagac accaataaaa aagccacgca tatcacgtgg cttctaacaa
     4021 ggtcaagagg aggttttatt tagtcggtgc tggaggttct ttatcaattg tccaagtcgc
     4081 ttgcacacct actaaattgg cacctgcggt tccagatgct gtaacgttat agcttgttgt
     4141 tgccgttaac acatcggttc tattaatcac tggatcactt gaagtgaata aatgggtata
     4201 tccgatatcg acaccaatgt tattagttgc ctgataatga gcgccagcag ataaagccca
     4261 tcgatctgta tccggtaaac gtacgtctct gtcaatatca ttcgtaggag ttggatcata
     4321 accaccaccc acacggagca ttaatttgtc attaacatga taattggcac caatcgccac
     4381 ccgcaaagta tcagtataat tctgtggtga tacacttatc gccttgactt gcgaaacacc
     4441 tccatttgca gccaatcttg gggcagctac attagtaagt tgtaccgttt taaagctgct
     4501 ccatgcggta taaaccaaag agcctaacaa tgcaaatttg tcattgacat catgataagc
     4561 actaatagtc actacatcag gtaaatcaat ggcattactg aataaatcat cgctttgaaa
     4621 tacgcccagt tgtggaaatg taggtgcaaa tatattgccc ggaattgcca atggtcctgt
     4681 cagccggcta tagccataga attcatgtct catctctgac tgatagttta ctccaagacg
     4741 agtatgatta tcgttaaaca tacccagaac gcctacatga aaacctactc caaaagagtg
     4801 tcctttattg taagtcagtg aatcagctaa catgggatta agatccaaag cattcattat
     4861 attaggtgcg ccaagtatac gattgtattt aacccgggca tattgcaaat ccaagccagc
     4921 acctaaagca acgtgctctg ttaatctacc cccaatttca ggtgagatat tggtggtaat
     4981 taactcagta aaagtggctt gatagcgcac tggagaatcg ggaccccaat ctgttgccaa
     5041 tccaaatggt gatactacac ttaacccaaa cgtagcattt tcacccagtg gcaaggcata
     5101 atgaaacgaa ggaacaaaag catcctcagc cccgttgata ttattgaaaa cttcagaata
     5161 aataaaagaa ggcgcaggag gcggattaat cgttgaataa gtactaatcc cgcttagctt
     5221 ggcagtcgga aataccccga caccggcaaa tacagcttgc tgatcgcgga ttaataccaa
     5281 acctgctggg ttgtaccaac ctgtagacgc atcggccgct tcagcagcca caccggcagc
     5341 gaagttacct gttgtatatc cagctccttc ggtatacaaa gaaaatccac ctgcgtttaa
     5401 tgtaccagta gctagaactc cgagaacagc agtactcacc agcgttctta agggtttgtg
     5461 cctaattttc actccacact cctttcgttt aataacatcc caattcatgg taataaaaat
     5521 gataataaat gaatttataa tgatttaaaa cggtaaaaaa aaattatttc ctattttctt
     5581 aaattgttac aaaagtttta atcataatga tctaatcaag ttaattatgg caaaatcaaa
     5641 ggcatatgat gaatttttat gcgaacctat ctttcccaac ccatgctttg tagactcaac
     5701 tcgtgactta ggttgtcttg agaaaaatct tttttaaatt gagacttaat ctacttgtac
     5761 aaatacttta gtccatgcta aggtgtatta attttgagcg gaatactaat catgcaacaa
     5821 gtagtcatca aatttggcgg cactagtgtt tcaagcagag ccacttggaa taatattgct
     5881 gcaattaccc aaaaacatct gaatacaggt gttcagcccg ttatagtctg ttctgcttta
     5941 actcaaattt ccaacaaact tgaaaaagcc atagaggcag cccttcttga cgagcatcat
     6001 agccttttca atgacattca gagcagtcac ctgaatctag cagaacaatt agaagtaaac
     6061 catcaattaa ttgctaatga tttgcaccaa ttacagcaat ggctaaccgg gatctcctta
     6121 ctcaaacaag cacctgcaaa aacacatgcg caaattttaa gtttgggtga gctgatgatg
     6181 acccgcttgg gtcatgcctt tcttgaaaaa caagggatta aagtaaaatg gtatgatgcc
     6241 agagaattgt taacaagcac accgaccctt ggtggagaaa caatgaacta tctctctgcc
     6301 cgttgtgaaa gcgaatacga cccggcattg gtagaaaaat ttctctcaac cggggctcaa
     6361 gccattatta cccaaggttt ttttgctgcc aatcctcatg gtgaaaccgt tttgcttggt
     6421 cgaggaggct ctgatacttc agcagcacta cttgcgggaa aactacaggc cgcttcttgt
     6481 gaaatatgga ctgatgtccc aggcatttat accgccaacc cgcatcaatt accccacgcc
     6541 cgattattaa agcaattaaa ttatgatgaa gctcaggaaa ttgcctcaat gggggccaaa
     6601 gttttacacc ctaattgcat accacctgtt cgaaaagcca atatccctat ggtagtcaaa
     6661 tacactcacc tgcctgaaca ttcaggaaca ttaattacca aagacattga tgaatcggca
     6721 cccttaataa aatccatcca ggttaagcac agcatactgc taatttctat agacactttg
     6781 aatatgtggc aacaagtagg atttctggct gatgtatttg ccgcctttaa aaagcatggg
     6841 ttttcagttg atttgctttc ttcttctgaa ttcaatgtca ccctatccct ggatgttaat
     6901 gccaaaatac atgatagacc agcaattaat gccttgctcg acgatttaaa ccaatttgga
     6961 cgcgccaaac tgatagaacc ctgtagtgct gtcagtttgg taggacatca catccgaact
     7021 gtattacctc atcttggccc cgcattggaa gtatttgagg caaaacaagt atacctgatg
     7081 tctctggcat cgaatgacct taatttgact tttgttgttg atgaatcaca tgccgataaa
     7141 ttatgccaaa gattgcatca tctactaatc gaaagtaatc ctcaaatctt ttattactcc
     7201 aaaagctggc atgaagaatt tggcaaaccc aatgttcgcc caacaccttg gtgggaaatt
     7261 gaaagagacc gcttgcttac aaccagcgca atacattctc cttgttatgt ttaccatagt
     7321 cccacccagg caactcgtgc aagacaatta tctgctctgg aatcaattga tagcttgttt
     7381 tacgctatta aagcgaatcc attcccatcc attttaaaaa ctctggaaaa agaagggata
     7441 ggttttgaat gcgtttctat tcaggaattg gaattagtgt taaaactgtt tcccaatatt
     7501 aaaaaagaaa gaattttatt tacacccaat ttcgctccca aatccgagta tgaatttgct
     7561 ttgcaaacgg gctgttatgt gactattgat agcttatacc ctttggaaaa ttggcctaaa
     7621 ttgtttaaag accgcgaagt aatcgttcgc atagatcctg gtacaggagc tggccatcac
     7681 aagcacgttt ctacaggtgg gaatgaatcc aaatttggta taacacaaaa tgacataagc
     7741 aagattctat cacataccaa agccaaccac attaaggtca taggcttaca tgctcattct
     7801 ggtagcggca tactctcaac ggatttatgg caacaaactg ccgtgatgct tgcctcccta
     7861 accgatcaat ttcctgaagt acgttcaatt aatttaggtg gcggtctagg tattgtagaa
     7921 aaaccgggcc aacatcccat tgattttgct gcgcttgatg cgcaattaat ggcagtaaaa
     7981 tctcaatatc cgggattagc aatctggctt gaaccaggtc gcttctttgt agctgaaagt
     8041 ggtgtcattc ttgccaaagt aacgcaatgc aaagaaaaag gaaaagtaaa atttattggt
     8101 attgaaacag gaatgaactc attgataaga ccttccttat atggtgcgta tcatgaaatt
     8161 atcaatttaa ctcgactaca tgaagaaaag gccgggtttg ctcatatagt tggcccaatc
     8221 tgtgagtcgg gagatacatt gggttatgac cgcttattac cagttaccag ggaaggcgat
     8281 gtgatattaa ttgcaaacac tggggcctat ggtcactgta tgagttcaca ctacaattta
     8341 agaccacctg ctcaggaaat agttctggaa taatggcatg cggttaattg atagtgaaca
     8401 aaggagccaa gcgacagatc ctgctgaatc atttatagtc caggctcctg ccgggtcagg
     8461 aaaaacagag attctgaccc aacgcttttt acgtttgcta agcactgtgc agtctcctga
     8521 acaaattatc gccttgactt ttacccgcaa agcagcaagt gaaatgagag aacgaatcgt
     8581 tcaggcatta catatggccg cgtcaaatca accggctgtc agcgcccatc aacaaagcac
     8641 tttaaatttt gcaaaacaag ctttacaacg aaataagcaa tatcagtggg atttacttga
     8701 gcaaccaaat cgcttaaaaa tcataaccat tgattcattg tgccaaagca ttaacagggc
     8761 aattccttta ttggaaaaac aggtggctta tccagacatt tccgagacac ccgattctca
     8821 ttatattagt gctgcacgtt gttgtatcca gtatgctatt gctacgccag agtaccagga
     8881 agcaattaaa accttgcttc ttcatgttga taataaacag gaacgattaa ttcaattatt
     8941 ccaggcactt cttagccaaa gagatcaatg gttgccgaca ttatttctgg cacgtgaaca
     9001 agaaaagtca acttttgagc aagctttacg ccttattgaa cagcatgaac tctctcgtct
     9061 aagaaatagc ctgccatcgg acttggctct agaactagtg cttttgtcgc gtgaattagc
     9121 cgttatagaa aacaaacctg attcaccacg atttatatta agagaatggt atgagtttca
     9181 aaaatgcgat cagcaaattg caggtgcttt aagcaaatta ctcttaacta gtgacaatca
     9241 attgcgtaaa tcgtttgatc atcatgtggg tttagttaaa aaaaattgtg caccaaatga
     9301 atttaccaga ataaaaacag ccagttcaga tttgtttact aaacttcaag attgtcctga
     9361 tttcctggac gcgctcctta aggtcaatca attaccaact cctgaatacg acaaagagca
     9421 atgggacgtt ttgcaagcac tctttttgtt actgccatta ttggttggac atttacaagt
     9481 ttcatttggt gaacataacg aagttgattt tactgccatc tctcaacaag ccttggcagc
     9541 tttgggtgat ttggataatc cgactgattt ggcactctat ctggatcatt caattcatca
     9601 tcttctaata gatgaatttc aagatacctc cattacacaa tttgaattac ttaccaagtt
     9661 agttcaagga tggttacctg gagagggaaa aaccttattc cttgtaggcg atcctatgca
     9721 atccatctac cgtttcaggc aggctgaagt tggattgttt tttcaagcga aagagcatgg
     9781 cataggacca gttcgcctca aatcattgga attaagatgt aatttccgtt cgacagaaac
     9841 aatagttaat tgggtaaatg atcatttcag taaaatattt tctcaacaag ttgatatcga
     9901 atcaggagca gtatctttcc atccttctgt tcatgtcatc gagaagaatg aatcaagcac
     9961 tattcaggcc gtgcaattta aaaatcgtga acaggaagca aggcatttaa ttgaaataat
    10021 taatagtgaa ttaaaccgca accctgaaca aaccatcgca gttttagtga ggacaagaac
    10081 gcaactccct gaaatcatta gcctacttcg ccaaaataat atcccttatc agggaacaga
    10141 catcgattta ctagccaatt tagtgcattt acgtgacgtc tggtcacttg ctcatgccct
    10201 gcttttccca ggaaatcgat tgtcctggtt atcgatactc cacagccctt atgttggatt
    10261 ggggttatct gacatccact gcatagccca atataattca agaaaatcaa tttatcacaa
    10321 tttgctccat ttagataaaa ttcaggatat aagtgaagaa ggcaggatta gagccaattt
    10381 ttttattcaa atcatgaacc aggctttgtt atgtcgatca catagtcgtc tatctgattg
    10441 gatagcggat accttaaaaa atcttcatgt agaaaagatc cttgataaag ctcagttaaa
    10501 tgatctggag caattatgga tgttattaga ccaatatgaa aacgaaggtc gaattcctga
    10561 tatgaacgaa tttttaaatg aattcaataa attgtatgcc caacaaacca ccccttccca
    10621 gttacaaatc atgactattc ataaatccaa aggattagaa tttgatacag tcattctccc
    10681 aggccttgga gctcaagcaa gtcgtggtga ttctcccatg ttgcgctggc ttaagctgcc
    10741 tacccagcac catggaaatc tgttactggt atcacccata aaaagtgcaa accaggatag
    10801 ttgccctttg tacgattatc tggatcaatt ggatgaagaa aaatccgcct atgaggcgca
    10861 aagattactt tatgttgctg ttacccgggc aaaatcaaga ttatatttgc ttgatcattc
    10921 ggcaaaatcc tataaatctt cctttcgaaa tttattaaaa taccaggaat tcattgcaga
    10981 tcctgacgaa gcccaacatg aagaacaaga attccctctg ccgaaattag tcaaactacc
    11041 tatagagtac tatcaaaaac agattcccag tttagttcaa tctcaagaga gttcatgccc
    11101 agagatatct tcgggtattc ctcggctaat aggcattgtg tctcatcaac tattacaatg
    11161 gatttgtgat aaccatcccc aaacaattga tcatgtgcca tggaacttgg taaataatga
    11221 acttaaaaca ttaggattaa accaggaagc ccaacaaact gctctatcaa tgataaaaaa
    11281 ccaaatttct caaatgtatg aaaataaaat tggcctctgg attttatcca gacatgaaaa
    11341 tgaacagaat gaatatgagc ttctggttga gtatgaaaat aaattggtta ctcgtattat
    11401 cgatcgtact ttcattgaaa atggcacaca atggatcatt gattttaaaa ccgggaaaga
    11461 agatataaac accttacagc aacatcgcca acaattaaat gagtacgggc gctatctgtc
    11521 agattgcaca aaattcccaa ttcgatgtgg tttgtattat cttgcaaatg ggcattggat
    11581 tgattggcag tatgaaatga gtgcttaaaa acacagcagc tatgcaattg cccaataaaa
    11641 tcatatgaca ttatcttaag ctattcgtct tactcatcag aaaagctata ctcaaaatac
    11701 attttctctc gagtagatgc attcatcaac ccagcaaaaa cgaatagtaa tggtactagt
    11761 gattagccga gacctaagtt atcattacct atttattttc acaccaggct aaaaaatgac
    11821 tattgaagat acagtaatta agttaattgg ctcattaaag gatcctttgc ttgaccttac
    11881 tggaaaagaa atgaatctac agtacaaaat aaccactagc aaccaggcta ttcatgttgt
    11941 cttgactgcc ggatatccta cctctttgtt agaaacgagc tataaaccaa tagtacagaa
    12001 aatagttcaa gatgaattcc ccaattacca agttactatt tctattcagc aattcataaa
    12061 agcacataaa actcagttga caggcaaagc cctgcgagga gtaaagaata ctatcgcagt
    12121 ggcctctggt aaaggaggag tcggaaagtc tactgtcaca gtaaatcttg cagcggcatt
    12181 ggcaaaactg ggcgctcgag tcggcatttt agatgcggat atttatggac caagcatacc
    12241 tttgatgctt ggcgaaacaa aacctgtaca agtaaaagat aattgctata taccggttga
    12301 agctcatggc atgcaggcca tgtccatagg ctacttgacc gatacaaacc aggcattaat
    12361 ttggagaggt cctatgctcg ccaagtcttt aatccaaatg ttagacatca ctctttggaa
    12421 tgagttggat tatttattca tagatctacc acctggtact ggggatattc aactcacttt
    12481 ggtacaaaaa attcctttaa cctctgccat agtagttact actcctcaaa atgtcgcaac
    12541 tttagatgca caaaaagcaa taaccatgtt ttccagaaca ggcattgacg tacttggaat
    12601 aatagaaaat atgtccaccc atatttgtag ccattgcggt catcaagaag caatctttgg
    12661 tagaggtggt gcagctgcgc tgtgtgacgc atatcaatgt acattattag gtcaactgcc
    12721 tctggacagc catgtacgta gacattgcga tgagggagtt cccacagcaa cacattcatc
    12781 aaatcaatta actgacacct ttattaaaac agcgatgcga actgcaattg agctcagcaa
    12841 gaaacccata aactatgctg acagatttcc aaagatagtt gtggaataga agtaatagct
    12901 tttctactat gtattattcc cttcagatga gtatataata cataaaaatt gagtttgcca
    12961 cctcaaagat aaccaagctc acaaaaaaca aaaataaaaa tgtcatgatt agattattaa
    13021 taataacaat ctgtgtatta tgttcgggac ttacgatagc caaatcaagt taccaaattg
    13081 atttaatact cttcgcacaa ccacaaaacg gtgttaaaag cataaaatca gattcgcctg
    13141 ttcccctcat tcctgtcagt ccaaatgcta ttcaactgca ttcggataat aataaaaaac
    13201 ataggcctta cactcttctg ccgcgatcgc aatcaaaact aagtgatcaa cattatcttt
    13261 taagccgtaa atcccaattt caaatcctgg gtcattattc ctggagacaa ccagcgaaaa
    13321 gtgacagttt ggttgcgcta cctaaaatca accataatgg atggcaaatt caaggtaccc
    13381 tcaaagtacg tcaaagtaat tactatctat ttgatgctga cctgcagtgt ttgtctccag
    13441 gtaatcagga gacttctttt actgtttcgc aaaaacaacg cttaaaagga agcacaattt
    13501 attatcttga tcatcctcag ttaggaatgc tagttaaaat tcataaaata gcttcatgaa
    13561 agctcatcca ggtaagcagg aatttacgta aaatttataa taatttctat gctaatttgg
    13621 attgaacatt ttttgttatt aattcagcaa ctgcattaaa acaaacttgt cgtggcgttc
    13681 ctgctcgcca aaataacccc aagatacgag agggtgccgg ctcagaaaaa ggaatacatt
    13741 ttaaatgatt agttgaggca gttagtgtag acaaagcggg cagtaaagtg acgcccattc
    13801 cagcttgtac cattaatctc aaggtttcca aactagtggc agtaaaatct gcgatgtcgt
    13861 cagctttcgc taattggcat acggccatgg cttgctccct caggcaatgc ccttcttcta
    13921 gcaacataat cggctgattt tttaagtcat tgatattaac tgatttggct tgggccaaag
    13981 gatgagtatt tgcgcaagca aaatagaact tttcttcata taaaatttga catgaaaaac
    14041 gcttatctat tggaagtgcc atgattgcaa catccagttc tccttgctcc aatttagtaa
    14101 ttaacctatg agttttatcc tctattaacc aaactttcag gcgagggtac tcatttttaa
    14161 gttcgggcat taccaagggc aacatgtaag gcgatacggt aggtataacc cccaaacgca
    14221 attcacctgt aaatggatct tccgactgac gagcaagttc tttcatttca tcaatcagga
    14281 ttaatatttt tctagttcga tctaataatt tggagccttg atcagtcagt aaaacctgtt
    14341 tgttggtacg ctcaaataac acaacaccta attcttcttc cagctttttt atttgcatac
    14401 tcaaagtggg ctgacttaca aaacacctct tagcggcttc accaaaatgc tttacatcag
    14461 ccaggatgac aaaataatgt aaatctctta aattcataat tgctcaataa taaaaaatct
    14521 attgtgacac aaattacaaa tctgcagtat aaaaatcatt tgtcaaattt tacgaaatac
    14581 atacagaatt attgattcca atgtaatagc aaatataatt catgaagaaa taaaaggtat
    14641 tgcagattat aaatttacac aaacataaca atgctatata ttccatcaat ttcaacataa
    14701 tataaactac tttgaacaat ttatcggcta ttaagaaatc aaaaatcacc atttggttgg
    14761 taggagtatt ttttttatta ttccaattct tcttgcaact atcctcgggg atcattgtag
    14821 gcgcaataat gcacgaagaa catatttccg cactcagcgc aggactttta agtagcgctt
    14881 tttactatgt ctatacttct tttcaaattc ctgtcggtat tctttttgat cgctacaata
    14941 cccgatattt gttatcggtc aatgcagctc tttgtgccat cggatgtttt gtatttgcct
    15001 cggggcataa cctcccgact cttttccttg ggcggttaat tattggcgct ggttcagcct
    15061 ttgcctttat tggtttaagc catttgttac gccaacattt tcccttaaaa caatacgctt
    15121 ttatgatagg cctatcagaa acgttgggat ttactgttac agtattagga atgattggca
    15181 tgggttctgt gatcagccat tttggatggc gttattttat taacatagca ggaatcattg
    15241 gattcttgat cgccagttta tgctggttgt gtatccccaa tagcaaacca atcgtttctt
    15301 ttccacacaa atataaacaa caaattatcc ccattctaaa aaataaatta gcctggttta
    15361 atggcctttt tgtttgtctg gaattttcag taattactgt ctttgccgcc atgtgggctg
    15421 ttcctttttt acaattaaaa ctggattgca ctatcaaaac agccagtata ctaacttcaa
    15481 tggtcttact gggcgctggc ttaagctgtc ctttattggg tcaaatttcg gtacatctca
    15541 atagacgaaa accgctgatc catgcttctt gtctgtcaac cgcgttatta ttaatacttg
    15601 ttttatattt accaactcaa aataagctat tgataggtat tttaatgttt tctatggggt
    15661 taacctgtgg tgcttatatg ctagcctatt caatttctaa tgaattagcc ccacctgaat
    15721 tcttgtccac ttgtactggc ttcaccaata ccttggcaat gctctcggct cccttacttc
    15781 aacctctcgt tggatttttt ttagatgtac tcagtaacca taaaagccaa tacagcttgg
    15841 ctgactacca actggccctt ttgattgttc cttttgccat gctattggca agcttattta
    15901 cttgcctcct gccagaaaaa tcaaattaga ctatcccaaa ttagtaaccg cttgtactgc
    15961 tgactccttg atttgtttaa aagtttgttt ggatataaag caaaatcctt ttggattttc
    16021 ttcagaaaag cctgcaactg ttagaggaaa atcatcctct tgttttatgg tattgactaa
    16081 ttttaatttt aacctgggca aaccaacgct aagtaaacat tgattatcag ttagggtttt
    16141 aatggaaaca aattgagaca ttttgacatt atttcctgaa gggtctcgcc aacctgctac
    16201 cgcagccccc gttgcatcag gttccttgag atttgcagaa taaaaaccat tatcattact
    16261 taatctgcaa aaaatagtgc ttatatcatg aggggaattt tgatccttgc taaaccataa
    16321 tttgctgaac catgccttat tacctgttac aaactgttct ttattatcta ctcccgtttg
    16381 cacgtgccca tttaatcgac tggtctgtac cgcttgcaac tctaccccag gtcttaatac
    16441 ctgtgtttca acatctttat tatcctgttt taacatatct actttaaaat taaatccttt
    16501 tgcattttcg actttcacgc caaaaatcca actatcattg atcggattgt agcgcaaaaa
    16561 tgagcgtcca acttgccagt tcaactgagt ggagctatct attttttcgc tggcctcata
    16621 aaacagaatt aactggttgg tttggccgtc aattaatgcg gttttagcat aaaaatcagt
    16681 accttgcctt tgcatctgaa aaaaatagcc gtatcgctca ccgctctcat tggtgaccat
    16741 acctgtaaat acccaacttc caacggttgg ttcattcgtt tggactactt tgtcttcctc
    16801 tggctcttcc actgcaaaaa cactgccact taaaaataaa aacatggata atagcaataa
    16861 ccaaattcgt tttggcatca tattagtaat aaccttgtaa ttgttgatgt gaccaaaatg
    16921 cttcccaaaa ggaatcattc aactttgctt caacgggtaa ataatataat ttatctgaac
    16981 taaatgaata acattttaca gcatcctttt ctgtcattat aactggtaga tcgatatcat
    17041 ttaaatcatg aggtttaaat tgatgatgat cgggataaga atatggatta aattttatac
    17101 ctaattgact taaagtagaa tagaatcgct gcgggttacc aattcctgct acagctgcta
    17161 cttcagaagt aaataaatcg ttactgactt cttcctgagt tgacaatcgg actatgtttt
    17221 taggaattaa ttccatagaa taagtacctt ctgcagcacc ttgattaaca ataacaaaat
    17281 caacttgttt taatcgtgaa tcaggttccc ttaatggtcc agcgggtaga caaaaaccat
    17341 tacccagttt tcgcatccca tctattacca caatttcaat agatcgcccc attttataat
    17401 gttgcaagcc atcatcactg ataataattt caaccgagtg cttgtctaaa aggtacctta
    17461 ctgcttcatt gcgcttaggc gcaataacca ctgggcaatt aatttttctt gccatcatga
    17521 gcggctcgtc gcctactaat tcagctgaat catttaattt tacttcgtat ggaaaatgtt
    17581 ttattgctgc tttgtaacca cgacttacaa tccctacttt taagcctttt tgctgtattt
    17641 ttttagcaat ttcaatgacc agaggcgtct tgcctactcc tcctacggtc acgtttccca
    17701 ctacaataat tggtataggg tacagctgtt ggcagaaacg ctgtaaatac catcgtctgg
    17761 ttcgtataac aattctgtat aaccaggaaa atggaaccag catccactgt aaaaaatggt
    17821 taccatacca tatcctgttc acaaaaaatg acattaaact accacttcct cattaatcct
    17881 gccaaattgc tgcactttat acaactgagc gtaatgacca tccatatcaa gtaattcttg
    17941 atgactacct tgctcaacaa tacgaccatg ttgcaatacg ataatcttgt gagcatgctt
    18001 aatagtcgac aatcgatgcg caatgattaa agtagttcta cctttcatca cttgctccaa
    18061 agccgcctga atataatgct cagactcact atccagagct gaagtcgcct catcgagtat
    18121 taaaattgga gcatctttca aaattgctct ggctatcgct attctttgcc tttgtccgcc
    18181 tgacaacaac accccatttt ctccgaccct ggtatcatag ccatcaggta attgcttaat
    18241 aaactcatca gcataggcca gtttagccgc agtaataatt tgctctcggc tcgcatcaaa
    18301 acgaccatag gctatgttgt tagccaaagt atcattaaaa agtgtcacat tttgactaac
    18361 caaagatatc tgcttgcgta aactttccag gctaagctgt tgaatgggca tgccatctaa
    18421 agtaatcata ccttgactca attcataaaa gcggggtaat aaactggcta tggtcgtttt
    18481 accacttcct gaatgcccaa ccagagcaac agacgttcct gcttcaatta caaaattgac
    18541 atcatgtaaa atattttgac tctgtcggta tgcgtggtat acatgcttaa actcaatttc
    18601 accacgtacc ttttctttta atatcaagcc attgtttctt tccaaaggca aatcaagcaa
    18661 attaaataca ctttctgcac ctgccaagcc tctttgtatg gttgcgttaa gagtagtcag
    18721 ggttttcata ggttttatta attgcaacat ggctgcgata attgccaaaa aagaaccggc
    18781 actgatagta ataacagtag ataaatgaat ggcagccata ataatcatgg caatcccgat
    18841 agcgatcacc aattgaacgc ctgaaacatt aatcgcctta cttatggcaa ctttcatatc
    18901 gtttttgcgt gaatattctg ttgctttatt aaattttgtt atttcatacc gctcaccacc
    18961 aaagatacga accactcgat aaccctcgat tgcctcacta gcaatttctg tgacttcgcc
    19021 catggttttt tggactttgt gactaattcg tcttactctt ttattggtat aattaacaat
    19081 gattcccaca aaggggatag ttaataaaaa catcaaagac agctgccaac atataaccat
    19141 cataacggtc agcaaaccga tgaccagaca tatattttga ataaaatctg ttagggcatc
    19201 cgcactgact tgtgccactt gctcaacatc gtacaaaatt tttgaaagaa gctgacctga
    19261 tgtggcttcg tcataataat ccgccggtaa gtgaataata tgagaaaata cggtctgcct
    19321 taatacttta actactgagc gggccaccca tgtcatacaa taactgccta aagaactgac
    19381 caatcctcgc agagtaatac cgattaaaac aatcagcggg atttgtttaa caaaatccag
    19441 atcaatagta ataaagcttt tatccaaaaa aagcttggtc atataggtaa agccggcgtc
    19501 aatgcccgaa tataggatat tcgccaatac tcctaataat aaaactggcc aaaaaggttt
    19561 tacatagctt aacaagcgtt tgtatagcag acgggattta atagggaggt tatttttcat
    19621 taattttgaa gttaatcttg tacgcaaatg ttatgaattg taactcttat tctgtctaag
    19681 cgccaatgtt ctatcatgaa gtccacctat tccatagtta gttttattct ggtataggca
    19741 caacgaaatc cagccttttg aacgttcaaa tctgaaactg tacctggttg agtggtcacg
    19801 gtggcgaatt ccaaaccata ctgcttggcc agattcagac gagcaaacag taattttttc
    19861 tgaaggcctt tccctcgata aagaggtaat gtgctggtaa cgcccaaatc acaagcatca
    19921 ccatgaattg ctattgtggc gccagcaacc aaattaccat gatcatatgc tgcaaatgct
    19981 aaaactcctt ttgcactgac atattggtaa aattgttcct gagcctcaag aaaaccaaat
    20041 ccaatagcta ctctttttgc ccactcttcc aattcagact gcttcacttc tcttataata
    20101 aacccatcat ccttggagtg agcaaccaga tggtaactct ttaaatcaag aactgaaaca
    20161 ttatttaact cagttatgta gtaacctctt tggcttaaaa aattaattaa atgatttccc
    20221 acaaaaggac ataactcaat atcgacacga ttatggccta acgatttata aaaattttct
    20281 atagcttcta tttcagcttt atattggtta ggctctgttt taaatcccca gcctactacc
    20341 tgggatagaa atgactcaaa accagaaaaa caagctgctc cgccgttgat ttccagaatt
    20401 tttccttgtg agtaccgctt tgtcacctca atgtgactct gcttgataca cccttccatg
    20461 cgaacagcca attccttagt aatatacatc gatttcctca ccaccaggat cagatagcat
    20521 ttaaacctga aacaatatcg tgaatcaact ccggcccctt ataaactaaa ccactgtata
    20581 cttgtaataa cgaagctcct gcattgatct tgtccttggc gctctccaaa ctgtcaatac
    20641 cacccactcc tatcaatgtc acatcattac caacatattg tttcaacaag cgcaagcagc
    20701 gagtagataa ttccatcagt ggcctaccac ttaatccgcc ctgttcttca gaacaaggca
    20761 aattttttac catttcccga gaacaggtgg tattcgttgc aataataccc tcgattccat
    20821 actgtaaaat gatatctgtc atttgtttta gtgtttcatc tgtttcatcc ggagatactt
    20881 tcaccactaa aggcacatgg cgtccatatt gatctgctaa ttttatctgt tctttttgta
    20941 actgggtgag taattcagca aaataatctc cttgttgtaa ctggcgtaaa tcaggggtat
    21001 tgggagagga aatattgata gtgacatagg aagcatgatc atatacttta cggaagcaat
    21061 aaagatagtc ctctgcagct tgatttaaat tagtctcttt attttttcca atatttatcc
    21121 ctaatattcc tttataattt gctcttttta cattttctac cagtacatca actcccgaat
    21181 tgttaaaacc catcctgttg ataatcgcat ttgcctctgc aatacgaaaa agccttggtt
    21241 tgggattacc tgcttgtccc ttaggtgtta ctgtccctaa ttcaatgaat gaaaatccca
    21301 atttggctaa tgcatccaaa tgttcgccat ttttatctaa accagctgcc agtccaactt
    21361 gatgagggaa tacaagaccc atagcatgaa caggttgacc ggctattttc cgaaaataaa
    21421 aaccagggag gtaatgtaat aaggataatg ttaatgaatg ggctttttct gcatccaatc
    21481 tgaaaagtaa agggcgcagc agtgaataca taaaattact cctaaagcag ggagaatcat
    21541 taataaagaa caattgaggt atgataacat ttttcgtgat gattttcgta ctgacgatta
    21601 ataggaagcg aaataaaatt aattcagata attttttatg catctgccca gataaatagc
    21661 gaaatgactt gctcattatg attgcaaata gtcctgttgc ataaacgagc ttattctgct
    21721 gataggctat tctccctgtc taaaagggaa aatcataggc gcgtctctat taatcgttgg
    21781 tccactcata ataaagctag aatcttaaac cacaaaaact gtattcctct gctgtattaa
    21841 tatgttcttc cttaacacca gtatcactct ttgatgtttt tggacctctt gatttttgtt
    21901 cgtttaaatc agaattaatc acttcttcat tagcactcaa tttatctttt tttaatccat
    21961 ttagaatttt ttgatgagaa ccctgatctt tgcattgcct ctccatcgag cttctgatta
    22021 ttggctcaac aggatgatct tgcgcctgat tatcagctat aaaatatgca gccagggtag
    22081 cagcaaacac tcctgtagca aagcctccca aggcaaacat aagcgcagta accgctggta
    22141 acgcaaaaat cccggataat ccaattgaca atggaactaa aacgatggct agagtcattc
    22201 ctagcaaaaa cacaggaatt atagagagag aaactacaaa atctgtagca agccaaccca
    22261 ataaagcagt cgctcctgaa ctagcaaacg aagtcaaacc caagcttccc caaaatttca
    22321 tgaaaccaaa tttttccccg gtaacattat cctcattctg ttgagataaa gtgttttcac
    22381 cttccaaaga gttggattga cactgtaaat catcatgact aatcagcaac tcagatttat
    22441 cttttgttaa ttcattgatc agaccatttc cattattgtt ttgtggcaaa ctgggattat
    22501 tttttaaccg atgcgcaaac gttgcagcaa ccgctccaag aaacaatcct gatacaacaa
    22561 gtagtaaacc aggtaaaata actggtgaag ccaacacagc cggagcggcg ctcataagga
    22621 gcataaaccc tatataaaaa cccaaactga aggagtcaac gataacagca gctaatgtca
    22681 tccccgacgc caaccataag ataattgcag ctgctcccat acttaataac gcagcaatca
    22741 tgtgagatct taaggaaggt gtaaactcac tgtttttctt atttgatact gccatttttt
    22801 cctccctgta taccagtcac ttactttaaa aaagtaatct caattaaaca acacattgtt
    22861 gcgcccaaac tcttagcatt gcattacctg atttatgcaa atgaaaagca gcctccctaa
    22921 cctccactcc atagttctcc tgttgcaagc ctagtaaaac cggtcctaga aattgtgaag
    22981 tgttaccttc tctatcaatc aatggaaaaa ttctcacttc tttggctacc cttgctaatt
    23041 cacgaattgc aatcaggtga aaatcaaccg tttgatcgtc aagatctgca aaaaaataat
    23101 gagaactcag agcaaaatca aaggtaaaat cggcaaacgg taaatgataa tcggtaagcc
    23161 cgaggtacct tccatcagcc ttgccttgtt cataatccgc aaaaaactgc ttcattcctt
    23221 cttgtctttc ctgaagcaat ttgtccaaac taccattacg gctaaagtca aattgatcct
    23281 gatgctgcct aacctcttca gccatattcg caaaaatcat ttttgctttg gcaagcagcg
    23341 tatctttatc caatacgaat aaagggtcac aactcactac tccttttttt ttcacttgag
    23401 tttgctggta attaacagcg ctaggaccgc aaccgtattc taatagtcgt aaattctcta
    23461 ctccctgggg taaatcaaac atctcacgat agtcttcaat tccatgcccc cataatacca
    23521 gttttctcat tagaaatact cctcttctaa atccatttgg attacactat ggctaaagat
    23581 taggtgattt ttttacccag gtaaagaatc attttaacca ctggccgggc taaaggtgtt
    23641 tttaagacaa aaatttaaaa agtttggaga gaaaagccac cttgcgattg aacaaatgaa
    23701 tatttaccat tcaaattctt tcaatttttc gcaataagac atccgtatat tatcaaatgt
    23761 atcagtgggt taaaaagatt gtcttggaaa taaacaggaa ggtgacaagt acgcttcatt
    23821 tgggtatagt ctagcgtatt tatcaacata acaggatgtc actcatgtac acaggcttag
    23881 actttcaact gggcgaaacg tatgacatgt tacgagacag cgtcaaccaa tttgcccaaa
    23941 ctgaaatagc tcctttagct gcacaaatcg atgagaaaaa tacttttcct aaccatctct
    24001 ggagaaaact gggagaaatg ggattacttg gcatcacagt aagtgaagaa tatggtggtg
    24061 ccaatatggg atacctcgcg catgccatag caatggaaga aatttccaga gcctccgcat
    24121 cagttggttt aagttatggc gcgcactcta atttgtgtgt caatcaaatc tttttaaacg
    24181 gaaataatga gcaaaagcaa aaatatttgc ctaaacttat aagcggtgaa tatattggtg
    24241 ctttggcaat gagtgagtcc aactcagggt ccgatgtagt cagtatgcaa ttacaagccc
    24301 gttctgctgg agacaaatat attcttgacg gcacaaaaat gtggataacc aatggtcctg
    24361 acgcggatgt tttggttgtc tatgctaaaa cagacaagca ggctggaagc aaagggatta
    24421 ctgcctttat tatcgaaaaa ggaatgcccg gatttaaaac agcacaaaaa ctagacaagc
    24481 ttggtatgcg tgggtcaaac acatgtgaat tggtatttga tcaatgtgaa gtgccttctg
    24541 aaaatgttct gggaacagta aatcaagggg tcaaggtatt aatgagcggc ttggattatg
    24601 aacgcacaat ccttgctgcc ggaccagttg gaattatgca agcttgtatg gatgttgtat
    24661 taccctatgt ccatgaacgc aagcaattta atcaggcgat aggtgagttt caatttattc
    24721 aaggcaaact tgctgatatg tacactgatt tgaatgcttg cagagcttat ctgtatgccg
    24781 ttgccaaggc ttgtgacaac ggtagggtta gtcgtaaaga tgctgcagga gttattttat
    24841 atactgcaga aaaagcaaca caaatggctt tacagtcaat acagattctt ggaggaaatg
    24901 gttatattaa tgaatatcct acaggacgtt tattaaggga tgcgaagttg tatgaaattg
    24961 gggcaggcac ttctgaaata agaagaatgc taattggtcg tgaacttttt aaagaaacag
    25021 cataaggaat gagacaatgg aacataatga tgtcgtgata gtcgcagcaa aaagaacacc
    25081 aatgggagct atgctaggca atttatctgc actttcctcc ccagagttag gggcagtagc
    25141 gcatatggca gcgttgtctc aatcaggtat tgctccggca gaaatcgatg aagtgatcag
    25201 tggttgtgta ttacaagctg gtattggtca agctcctgcc agacaggcag caataaaagc
    25261 aggcatacct aattcgacag gagccacgac cattaataaa atgtgtggtt cgggcatgaa
    25321 ggctgtgatg cttgcgcatg atttaattaa ggcaggtaca gctaatgtaa ttttggctag
    25381 cggtatggaa agtatgagca atgcccccta tttattatcc aaagcacgag caggatatag
    25441 actgggtcat ggtgaactga aagatcatat gttccttgat ggattagaag atgcctatga
    25501 taaaggacaa ttaatggggt gtttcgctga aacaactgca aaacacttta atttcagcag
    25561 agaacaacaa gacgaatatg ccttacgctc aatgaccaaa gcgctaaaag caatagagaa
    25621 tggtgccttt cgagaagaaa tcgcgcctgt caccttaact acccgcaaag gagatatcac
    25681 ggttgacgtc gatgaagggc ctgagacagc caaacttgcc aaaatacctc agttaaaacc
    25741 agcctttcaa gcagatggta cagtcacagc agccaattca agttctattt cagatggcgc
    25801 ggccagttta attttaatga gtgcctcaaa cgcagaaaaa cgtggcatta agcccttggc
    25861 aaaaatcata gcacatgcca gccattccca agccccacaa tggttcacaa ccgctcctgt
    25921 tgatgcaatc cgtaaggtaa tgaacaaagc gtcatggaaa caagatgatg tggatctttt
    25981 tgagattaat gaagcgtttg cagtagtagc gatggcagca attactcagc ttgaattgaa
    26041 cccggaacaa gtcaatatac atggcggggc atgcgcttta gggcatccca ttggtgcttc
    26101 gggagctcgc attctggtta ctttaatgca tgcgctaaaa caccagggta aaaaacgcgg
    26161 aatagcttct ttatgtatag gcggtggtga agcaacagct atggcaattg agctaattta
    26221 acggttaaag atgacctggg taaaaccttt atttttaccc aggtttttat attaaaactg
    26281 gttgctattt ttatttgcga ccagataatg ttttacctat caaaaccaac ttggttataa
    26341 caataataaa ggatgtacat gctaagacat agtctcattt accttctact aagtattctt
    26401 attgtcgtat ttgcaaaata tgcccatctt atcgtagttt acatcgatat gtttttcact
    26461 tacgttaatt taaaattaac acctatattc agccaaacag gttggggact gattatcagg
    26521 aaaattctag tccttatgtt attacccata atcatcgcag gaatcccagc gctaatttac
    26581 cgggcactca aaggaaaaga catgccccat tttattgcga ttgtctggat aatatggacc
    26641 atcatagtct tgagtgatat tttgattcgg taaggataaa aaatggctaa gttaactact
    26701 caaatcaata ccagcagtca ggaattcaaa aataatcagg cgaatatgca agcattggtt
    26761 actgatttaa gagaaaaaat acatcaaatc tctctgggtg gagatgaaaa agcacgtacc
    26821 aaacatcaac aacaaggcaa actacttccg agagaacgct tacaccagtt gctggatcca
    26881 ggtagtcctt ttcttgagct atctcaactt gctgcctacc aagtctatga agacacaata
    26941 cccgctgcag gaataatcac tggcattgga agagtggcag gaaacgaatg tgtgattgtg
    27001 gtcaacgatg ccactgttaa aggtggaaca tattatcctc tgaccgtaaa aaaacactta
    27061 agggcacaag aaattgcatt gataaaccat cttccatgca tctatctggt ggattcagga
    27121 ggtgcctttc taccgcttca agatcaggtc tttgccgata aagaacattt tggacgtgtt
    27181 ttttataatc aagcgcaaat gtcagcatta aatattcctc aaatagccgt ggttatggga
    27241 tcttgtacgg cgggaggagc ctatgtacca gccatggctg atgaatctat tatggtcaag
    27301 aatcaggcaa ccatattcct tggaggccct cctctcgtta aagcagcaac aggagaagta
    27361 atcagtgcgg aagaattagg aggagccgaa gttcactgtc gccactcagg agtatctgat
    27421 cattatgcgg aaaatgacgc tcacgcgctt tatttagcgc gagtcgccat tagcaattta
    27481 aatcgtaaaa aaccagattc cattcataga gtggatacag ttcctcctct ttatgatagc
    27541 gaagatctta ccggaataat tcccactgac cctagaaagc cttttgatat aagagaaatt
    27601 attgctcgag tggtagatgg ttcagagttt gatgaattca aagcactttt tggaacaacc
    27661 ctggtttgcg gttttgctcg cctatatgga tatcctatag ggattattgc taataatggc
    27721 atacttttta gtgaaagtgc gcaaaagggc agccatttca tagagctgtg ctgtcaacgt
    27781 aaaataccac ttgtattcct gcaaaatatt acaggattta tggtgggctc caaatacgaa
    27841 gcatcaggta ttgcaaaaca tggagccaaa atggtgacag cagttgccaa tgctaatgta
    27901 cccaaattta ccatcattgt tggtggaagt tttggagcag gtaattatgc catgtgtggc
    27961 cgggcatatg ctcctcgttt cctatgggcc tggcctaatg cacgcatctc agttatgggt
    28021 ggtgaacaag cagcgaatgt tctcgcgcaa atcaccagag agaaatatgc aaagcaagga
    28081 aaagaatggt cactcgaaga agaagaacaa tttaaaactc aaatgagaag tcagtatgaa
    28141 actcagggaa atccttacta tgccagtgcc aggctttggg atgatggggt catcgcgcct
    28201 caggatacac gcaaaatact cggacttgga ttatctgcag ccttaaacgc accgattgag
    28261 gatactcgtt tcggtgtatt tcgcatgtaa aaggaaagga acaataggca tgagtgattt
    28321 attatatgaa attcaagata aagtaggttt acttactatg aatcgaatta gcaagcataa
    28381 tgcgttcgat aatcaactgc tgactgaaat gagaatacga cttgactccg caataaatga
    28441 tactaacgtt cgcgtcatag tacttaaagc gaatggaaag catttttcag ctggtgctga
    28501 tttgacctgg atgcaaagca tggctaactt tactgaagaa gaaaacctgg aagacagtct
    28561 ggtattaggt aatttaatgt atagcctcag tcaaagccca aaacctacta tcgccatggt
    28621 tcaaggtgcc gcttttggtg gtggagctgg attggcagca gcatgtgata tcgccattgc
    28681 atctacttca gcacgttttt gtttttctga agtgaaatta ggtcttattc cagcagtaat
    28741 tagtccctat gtagttagag caataggtga acgagcggca aaaatgttat tcatgagtgc
    28801 tgaagttttt gatgccacac gtgcatattc tcttaattta gttcagcact gcgtccctga
    28861 tgatacgttg ttggagttta ctctcaaata cgcctcgcaa atcagtaata atgctccaga
    28921 agcggttaaa aactcaaaac aactggcaca atacgtcgct aataaaaaaa tagatgaaga
    28981 actggttcgc tacactgctt ctctgattgc tcataaaaga gtatctgatg aggggcaaga
    29041 agggcttaaa gcatttctca ataaagaaat acccaactgg aataaaggtt ctggacatgt
    29101 ttaataaaat attaattgct aatcgaggcg aaatagcctg tcgaatcatt aaaacagcac
    29161 attctatggg tattcaggct attgccgttt attcagcagc agatagaaac agccttcatg
    29221 tgcgattagc tgatagcgct tattatattg gagaagcgcc agctaaagaa agttatctga
    29281 atattgacca tattattcag gcagctaaag aaagtggagc acaagccatc catcctggct
    29341 atggattttt atctgaaaat cctgattttg ccaaagcctg tgaacaagca ggtattgttt
    29401 tcatcggtcc ctccattaaa gcgatggaag cgatggcatc caaacaatta gctaaacaac
    29461 ttcttgaaaa aacgaaagtc ccacttacac cgggctatca tggcggtgaa cagtctgaag
    29521 aaaaattgtt gagcgaagct aaaaaaattg gatttccagt actgattaaa gccgctaatg
    29581 gtggtggtgg aaaaggaatg cgagcagtac atgatgaaaa agagtttcat gatgcattag
    29641 caggagccaa aagagaatct atggcctcct ttgcggatga tactatgata attgaacgat
    29701 tagttcttaa tcctcgtcat gttgaagtac agatcatggc ggacaatcat ggaaatgttg
    29761 tacatttgtt tgaaagagat tgttcaattc agcgcaggca tcaaaaaatt atcgaggagg
    29821 cccctgctcc caatttatcg cctgtgcttc ggcaaaggct ggcagaagca gcctgcgaag
    29881 tagcccgctc catcaactac agaggagctg gaacagtaga gtttttggta gacggtgaag
    29941 ataagttcta ctttatggaa atgaatacaa gacttcaagt agaacatcct gtcacagaaa
    30001 tgattacagg actcgatctt gtcgcttggc agataagaat tgcagcgaat gacacattac
    30061 ctttgcttca aaatcaaatt caagctcaag gccatgcaat cgagtgccgt atttacgccg
    30121 aagatcctta tcagggtttt ataccctcta ttggtcaact tcaattctta aaagaaccct
    30181 ctggcgatgg catacgcatt gatacaggtg ttactttgtc ttcagaaata acaaggtatt
    30241 atgatcccat gatagccaag ctgattgcct ggggacataa tcgtgaagaa gcattacaaa
    30301 ggttggagcg tagcctgtct cactatgaca ttggcggggt aaaaacaaat ataccgtttc
    30361 taagagccat ttgccgacat gcaaaattta aagaggcaaa attaagtact gattttttag
    30421 agaaagaaaa cataaatctg cctaaaccag ataatgagtt aggtatactc ctggccataa
    30481 gttgtgatta tttaggtatg gttaatcgaa ctatggatcc cttattgcaa gatgcatttg
    30541 gctggcaaat gcatctttcc agccattggg tatggcgtta tcaattgaac tccacgataa
    30601 ttgaaactca gattacacct atagataata aaaaatttaa agcaaaaatt gaaaacaagg
    30661 aaatggtcat ccatgccagg tatgacattg accagcttat tattgaaatt gatcaaaaat
    30721 cagtgaaagc cagagtagaa aacaaagatc accatttgat cttctatact gacaaaggcc
    30781 aactttcaat cgaacgcttc tactggagta aactggatgc tcaaacttct gcccataaag
    30841 gccaattaac cgcgccaatg cctgccactg tcgttgctat tttaaaaaat attggtgaac
    30901 aggtaaaagc tggtgaaagc ttaattgtac ttgaggcaat gaaaatggaa cacaccattc
    30961 atgctccaat cgatggtata ttatcagata ttttttattc tgtaggttcc caagtgagtg
    31021 agggagcaga actgttagca cttagcgaat cggatactta agagaatatt tatggactat
    31081 cctcagcatg tgacaataat cgaagtaggt cctagagatg gcttacaaaa tgaaccatct
    31141 tttctccata gtgataaaaa aattgaatta atcaatttac tcagtcagac agggctgaaa
    31201 gagatagaag tcaccagttt tgtttcagca aaagcaattc cacagctagc tgacaatgaa
    31261 gaagtatttc aatctattaa taagactccc tccatcaact actctgccct ggttccaaac
    31321 gaacgaggaa tgcttaaagc cctggaaatg ggagtacaaa atattgcagt attcactgct
    31381 gcaagtgaat tattcaatca acgcaatatt aattgttcta ttaaggaaag tatagaacgc
    31441 tttaaacctg ttttagcatt agcaaaaacc aatcaaatta gagtgagagg ctatatctcc
    31501 tgcgtattag gctgccccta tgaaggatat atacaaccga gtcaggttgt tagtgtcact
    31561 aaaatgcttc ttgatctggg ggtacacgaa atttcgttgg gagatactat tggcgtggga
    31621 actccgagac aaactcagtt gcttcttgat gccatactac ccatacttcc aatcacacaa
    31681 ttagccatgc atttccacga tacttatgga caagccgtag caaacattta tgcctccctt
    31741 gaatatggag ttaaccgctt tgacagctct gttgcgggtc ttggaggttg tccatatgct
    31801 cgaggtgcca gtggcaatgt tgcaacagaa gatgtacttt atttgatgca tggattgggt
    31861 attgatacag gtatagatat attcaaaatt gttgctgccg gagatatgat ttgcaaagca
    31921 ttaggacgaa aaaaccaatc caaagtagct aatgctatgc tagccaatcc atgtaattag
    31981 gaagttccat cattattgac tttgataagt actaaaaatc ggaaacttaa agagaagtgc
    32041 ccagttttgt gctagcctat acttataaac aaattactga tgaaattttc cagtgtaaaa
    32101 cccaacattc aaaacagaac tgtgagagaa attttcatga acgacaaagt atggacacct
    32161 aaaaaccctg aacaaacaag aatgtggcaa tttatggatt ttgccgcaaa aaggcattgt
    32221 caggtttttg aaaattatca agacttacat acctggtcag taaagcatcc tgaatcattc
    32281 tgggaaactc tttgcctttt ctttaaattg aactttgata caccaccgca ccaaattctg
    32341 aattattatt ctgagatgat tgaagcccgc tggttttcag gggctcgctt caatttcgct
    32401 gaaaaattat taacccgtag agataatcac cctgccctaa taagccttga tgaaaatgac
    32461 tctaaaagtg tcattagcta tgctgaatta tatgaaaaag tagcccaatg cgctgcaggt
    32521 cttaaagcag ttggtgtcgc cactggcgac cgggtggcgg cagtgatgcc taatacttct
    32581 tacacaatca ttgccatgct ggcagcaact tctttagggg caatctggtc atcctgctca
    32641 cctgactttg gtacccaggc tgcacttgac aggataggac aaattgaacc taaagtactc
    32701 tttatttgtg atggtcatca atatcaaggt aagaaacaca gcagctctga aaaggtcgta
    32761 caacttactg ctgcgattac ttctctaaga caggtagtaa tttgcccgaa tattaatgag
    32821 gaaattaatc tcgaacaact gtctaacgcg tcgtattgga atgattttat aaaacctgaa
    32881 aaagaatgtg atttcatttc tctgcctttt gatcatccag tatatatttt gttttcttcg
    32941 ggaacaacgg gtaagcctaa atgtatcgtt catggcgcgg gaggtacttt aattcagcat
    33001 ctgaaagaat taggtcttca ttcagatatt aaatccacag acaatttatg tttttatacg
    33061 acgtgtggct ggatgatgtg gaactggact gtttctgcgt tggcattagg agctaccatc
    33121 actctatatg aaggttctcc aacctacccc acagataata gaatgtttcg actgatagat
    33181 gaagaaaaag tcactgtatt tggaactagc gccaagttca tatccgccat tgaaaaagcc
    33241 ggggtaaaac caaaaagtga atttcaatta tcggagttgc gctgcatctt gtctaccggc
    33301 tcccctcttc tgcctaaaaa ctatgatttc gtctatgaac atattaaaaa agatctgcaa
    33361 ttaagttcta tttcaggagg aacagatatt atttcttgtt ttgcattggg aaatcctatt
    33421 ctcccggttt atcggggtga attacaatgc attggattag gtatggaagt tgcggtgtat
    33481 gatgagcaag gccattcaat caaacaaaaa cgtggtgagt tggtttgcac taaacccttc
    33541 ccttccatgc cagtctgttt ttggaatgat aaggatagac aagcatataa acatgcttat
    33601 tttgagcgct tcccaggggt gtgggcacat ggcgatttcg ctgaaataac tgcccacaat
    33661 ggattaatta tccatggacg ttccgacgct atattaaatc caggaggagt gcgtatagga
    33721 acagctgaaa tttacaggca agttgaaaaa gtagatgatg ttgttgatag catagtcatt
    33781 gggcaagatt ggcaggatga tgtacgagtg gttttattcg ttaagttgcg tgaggggaaa
    33841 aaactggacg atgaattaat caatctcatt aaaacaacca ttagaaaaaa tgcctcacca
    33901 cggcatgtgc cagctaaaat actacaagtc ccggacattc ctaaaaccct tagcgggaaa
    33961 gtagtggagc tcgctgtcag gcaagttgtt catggtcaat cagtaagtaa cttacagtct
    34021 ttggcaaacc cacaagcatt agaatatttt aaagacaggg acgaattgaa aagctaaaat
    34081 tgcgtgaaaa tagctatttt cagcacgaat ccacattaaa attcatctca tatcaaccaa
    34141 tagaaagggc aactgcaaga gttgcccttt catgtaaata cttattattt gaatgaaaaa
    34201 aattttaata atcctcataa tttgaggaaa actcatttac ccgaatactt taaaaccatc
    34261 ccctaattta caattcattt gataacttcc taatcgctca taactacttg cattcagcac
    34321 agtggtttta attgaagcat caccctcaga atctaaatta aatttactgt aatcaaacat
    34381 aggtgtacct cgagctaccg gatcatcaag agtaaaatgc ctatctgagg ctgtaacacg
    34441 gttatcaccg actaccataa ctgcattcgg agtaactgaa actattgcag aactcaatgg
    34501 catttccgaa gtacattttt ttaagctaat tacaaaagtg attcgctttc cttcagctat
    34561 ggcatctact atattttgaa aagtttttaa ttctactgct gcctgcgctg tgaatgagca
    34621 caaggtaaaa actgctaaaa acatcttttt catgaataat gttccttgta ttaaaaccgg
    34681 cttaaggttg ccaataagaa tttatcttgt caatcctcaa ccaaaataat tattttcaga
    34741 ccgaatgaca ccgcgtatct ggttaatgcg aattacctct ttttatataa accaaaagcc
    34801 aaccaataat catgaaaaac aaatcaagat acctcaacag agttaagatt tttctttgtt
    34861 tatgaatcca gtttgtgtat acttgttcat agcgtcgatt tgaaacacag cttgcggacg
    34921 ctcaaaagat ttattcctct gtttaagaac atgaaaactc taaaatcgag gtaaaatgca
    34981 attattgctt tttattaaga attattgttt ttcaatgctt cagatatctt ttgaaaatac
    35041 ggctaaagcg gttgctttct aatccccact ttatgccccg caagcgatat tcccaggtta
    35101 tagtaatgat tgaattatgt gggctaaaaa aatctttctc tggtaaactc gccttaaacg
    35161 atattaattt atttattcag gaaggtgaaa tttttggagt tatagggaaa agcggggcag
    35221 gaaaatcaac attactgcgt tgcatcaata tattggaaaa acccgacgaa ggtgaagtca
    35281 ttattgatgg gcagaatctg atgagccttt ccagaaaaaa tttagccttg gcacgtcata
    35341 aaattgccat gatcttccaa cattttaatt tgctaaattc aaaaactgtt tttgataaca
    35401 tagcccttcc gatgagaatt caaggtatag acgaagagtt gatcaaacag aaaattgaag
    35461 agctgcttcc tgttgtcgag ttaacagata agaaagatgc tttcccctca caactcagtg
    35521 gtgggcaaaa gcaaagagta gcaattgcgc gagcactaag ttgttctccc aaagttctat
    35581 tatgtgatga agccacttca gccctcgatc ctgcaactac agacgccata ttatcactac
    35641 taaaaaaaat caatgaattg tatggaatta ctattgtctt gattactcac gaaatggatg
    35701 tcgtaaaacg tatatgccaa agactgtctg tcatggtcga tggcaagatt gttgaaacta
    35761 ctgctttatc taatattttc aataagccag agagtctcgc tcgcaaaatg ctttacgcac
    35821 aaattagccc tgaactccca acctgcctta ctcgcaggct ggcggactat gctactgaaa
    35881 aacctctcgt gagattattt tttcagggcg aggaagcaac ggtaccattt attagccaaa
    35941 ccagcagaga attaaacatg gacatcaata ttctactggc aaatattgat cgtttcgatg
    36001 gagtgacttg cggtgttttg gttgttgagt taacagcgaa tcctttattg cttgaagcgt
    36061 ttatcaatcg ttgcgaacaa gccggtatta ctgtggaggt tttaggctat gtcctaccag
    36121 atggtttatg atttacttag cgcaacagga gaaacacttt atatggtgtt ttttagtact
    36181 ctatttgcag tactccttgg cttgccctta ggtatattgc tgtactcatc atctcggata
    36241 aaaccaaata ttaaattgaa taaaatttta tctgctctga ttaatatttt ccgttccatc
    36301 ccgtttatca ttttacttgt tgcaattatt ccattcacga ggctaattgt tggtaccagc
    36361 atagggatca atgcagcaat cgtcccatta acagttggtg cgactccttt ttttgcaagg
    36421 ctagttgata atgttctcca atcgctaccc cctggtctta tagaaaccgg gtattcaatg
    36481 ggagctaaca cacgccaaat tattttacat atcatattac ctgaggcaca atcaggatta
    36541 attcattcca ttacagtaac agcaatcact ctgattaatt attcagccat ggcaggagct
    36601 gtgggtgccg gaggattagg tacattagcg attaactatg gttatcaacg ctttaatgct
    36661 ggcattatga tagctaccgt gattgtatta attattttgg tccaactgat acaaatggtt
    36721 ggagactact tggccaaacg ttgtacacat tattaattgg gaggttcaat gagaatttta
    36781 agttgtttgg ttttaatcat tagcctggta gcttgcagta gtaagccagc tcccaatact
    36841 ttagtcatcg gcacaattgc tggccctgaa acagaactta ttgaaacagc aaaacaagtt
    36901 gctgaaaaag agtatgggct cagcataaaa attgttgaat tcaatgatta taatttgccc
    36961 aatgaagcac tgcaagatgg cagtctagat gctaatgttt accaacattt accttattta
    37021 aaagccgcta tactgtctca tggttatgat ttgcaggcaa ttggcagaac cttcgtttac
    37081 ccaatgggta tttattcgaa aaaatataaa acactcagtg aattacctga aaatggaata
    37141 attgcagtcc ctaatgaccc tagcaatgag atgcgcgcct tcttgttatt ggagaaagct
    37201 catttgatta ctcttaaaaa tacaacgaat agcggtattc aagatatcga aagtaacccc
    37261 aaacaattca aattcaaaga gatagacgcc gctcaattac ccagagtact ccctgatgtt
    37321 gacgctgcag taattaacac gacgtttgcc ttgccagcag gattaagccc atcaaaagac
    37381 gctttattta ctgaaggcaa agattcacct tatgccaata tcattgtgat acgacgtgac
    37441 actgaaaaaa ggccacagct ggaattgttt gtgaaggcat tgaattcgga agaagtgaaa
    37501 gagaaagcaa aaaaactgtt tggagaagat gcaataccag cctggtaatt attaataaat
    37561 tagcttaatt tttacttaat aaaaaactgt tatactttat ctagataacc ttattgaggt
    37621 atttgatatg gctttttata attctatccc tttaattggt aaacacttaa aatcagagag
    37681 ccgtgcaaaa aaatacactt ctgaagaatt taaaaggcta gagagagaat acagagatca
    37741 catcggtttg ccagaaaact caagctttag ttctgacgca ttcgataagc ttgtcagctc
    37801 cagagtagat gatttccggg cttttccgaa aaccaactta cccgggggat tgctgtttca
    37861 tgataaactg aaggaagcca atactaccga aggtgaaaac atccgggatg tcttaagtaa
    37921 aacagtgctg gcaccaactg aaaaatttaa aacagcacaa aaaaatttta cagaatcaac
    37981 ttcaacattt aaagagctca ccaaattaat tccaagcaaa ttccgagccc aggatcttgt
    38041 tggcaccatg aaggaactga ctgatgatgc tcgtaaagcg ataatagaac agcaagcgca
    38101 tgaaatccaa atgttgaaag aaaaattgga ccctcagaaa gaaggacaag ctggtaatta
    38161 cgtatccgat tttaaaaaag ctctgggcct taaggataat gacgaagtta agaaagttac
    38221 agaaggcttg attaaggaat tggaagaaac ccataaaaaa caactgggtg aattcgataa
    38281 atccacaagc gactcgttaa accaactaca taaagcctct gcgagtgaat catcagagta
    38341 tctgtttctt gccaatctgt atgaaaacaa taaggaaatg cgtcaaacgc ttgaaaagtt
    38401 atatgccaaa gaacgccaaa gaaaagggct tcctcctgaa tctacaacag ttcaagtagg
    38461 tatatcagaa aacaaaattg atctcagtgg aatcaaactc aaagatattc ctgtcattaa
    38521 aagtattaca gggagagaga ttcatcaaca accggatggc agttttgcaa tccagatgcc
    38581 gatgataaac ccattctact atcaggatcc aagacaaaat gtcaaaacag acatgatgct
    38641 cattgctcaa gctataaaag cctcaggtta tgactctatt gaaatgaatt gtaattttcc
    38701 aaatagtgaa aaggtagcga tggaaagggg caggcaagct tttgccgcct gtattgaagc
    38761 aggctttcct gttgacaaga tcaccattaa tgttaacggc aaacctatga aggcggacga
    38821 attgtttaaa gaacatccga aaacatatca ggatattatg tcaagagcct caaaaatctc
    38881 gaaagattac gaggacatca aagcgcccaa aacatcaact gctccagtaa acaccgtcgc
    38941 cgtcaaacag gaaatcgcca cattacgcgc caaacagcag caagaagaac gagctcaaac
    39001 acaacctgtt gatgaacaac gacatggcat gacccccaat aattgactta taagttgaat
    39061 ttggattgtt taatccaaat tcaacaaatt cattatcctc cccgaaattt gctcaaaaaa
    39121 tttcactacc tcaaaccatc aatttctgat attttatcca ttctagacac ttgaatttta
    39181 aatgacagat tctttgtacc gccacttatt agaagtatcc ataaagtgga taccggttac
    39241 cttggataaa ctgtgtcaat aaggctattt gaggtggcat taaaaaataa agtgccagta
    39301 taactattcc aggtattttg gtatcatcga gaatagatat ggaattcaat gggttagcta
    39361 aggagaaagc tatgagtact cgtcgctttg atgaatttga caatgaaatg gatgatttta
    39421 gtgatgctga acctgttata actgaggcaa gcccttcaac aaaattggaa agacgtagaa
    39481 aaatagaaga tctgttggag gaaaagcggc taagagaaga actggaagag tttggttagt
    39541 tggaagaaaa ttgaggcata taaatgtcaa accttacagt cttaccatca attgaggcat
    39601 ctggcgggag taatggtttt accttttgcg gccacttgac tacaactctt gacttaacat
    39661 gagataaaga caacttgatt aactccattg catcataatc cgttccaatt agttgctgca
    39721 aaacttgcat ctctttctta accaaagccg atttgtttcg ttctgggtgc atgggatcaa
    39781 tgtaaataat atcgggataa tccttttctt gcaaactatg caggaatgaa atagcgtcac
    39841 ttgcaatcaa agacaaacac attttttgaa tgtctgcttc gtttctacga gatagtgcat
    39901 cagcaagcag tgcagccatg accgggtggc gttccagcat aagcacatcg gcaccaaagg
    39961 ttgccaaaat agcggcatct tttccccaac ctgctgtagc gtcgaaaatt ttaatgcctt
    40021 ttgttggctt gcaagccctg atcaagccct gttttttacc ctcccctctt cttttactcc
    40081 aggtcatagc actgaaatca gcaaacataa gagaaaaatt tcgaattttt aaagttaatt
    40141 tttcaggagt aacaaaaagg caggtatcag cattttgatc taactgtaaa ttcaattgat
    40201 cagctaacaa ttgtgctttt tctttgagct cattattttc ataaccaact gcttttattc
    40261 tattcattct tacaataata attgattgac atactgtaac agggaattat aaattggtac
    40321 tttaggatat tgtgccaact cagacatatc agtcatatta gaaagtacaa taataggctg
    40381 ggcaccaact gccaatgctg cttgtatatc tgaaactcta tccccgacaa aaggcacgct
    40441 atccagtgaa caatttaact tttctgccag agcctttagc attcctggct ggggttttcg
    40501 gcaaaaacag ttgtcttcag gtaaatggat acaatactct agggcgtcaa tttgcccccc
    40561 tgcttctgca agcacctctc tcaacttaca atgaatggca gataaatcat cctcagagta
    40621 tagccctctt gaaactccag attgattggt tgcaacaccg attttatatc cggcagcact
    40681 taagcgagct atcgcagtaa tgctatcggg tataataata agctcatcaa cattcttgat
    40741 ataattgatt gagtcagtgt taattactcc atctctatca agcagtataa ttcgattcat
    40801 aagtgctaca acattgaaat gtcagctaca ccctgcaata actcttgtaa tcgctgcagg
    40861 agggcaagcc tgttctgttt caatgactca tcttcaacca taaccatcac atgatcaaaa
    40921 aaagcgtcta ctggctcttt aagactagca agaagcttga gtaatgcttg gtaatctcca
    40981 gcaatgtata aagtttctac tgttttagta attttattaa gatgatcaaa caatgctttt
    41041 tcagcgcctt cctctataag ctcttcctta atatctactt ttttatccgc atatgctgcc
    41101 tgagccaaga tattgccaac acgcttgcag gcagctgata atgatgtcgc ctctggcata
    41161 gtaataaatg actttaaagc gaatagacgc ttatctaaat catataacca atcatcctga
    41221 cgagcacgga cagcatgaac caaatcagcg ctgatacctt gattttgata ataagattga
    41281 agtctctcaa gaataaatgg ttttaattca gccactaaat tcttgtcttg agggagtcga
    41341 tcaccataat tagccaaggc ttctttaatc aaagttgata aattaagcgg tgctgatatt
    41401 gaaatgagta gacggactac agccaaagcg tggcgtctta atttaaaagg atcttttact
    41461 ccagttggct tttggccaat cgcaaaaata cccgccagag tatcaatacg atccgccagg
    41521 gaaagtgcct tgcccaaatc agttgatggt aaatcatcac cagaaaatct aggcatgtat
    41581 tgctcattta aagcggtagc aacaacagtt tcttcgccat cattaagagc ataataatat
    41641 cccataatcc cttgcaattc aggaaattcg cctaccatac ctgtcatcaa atcacacttg
    41701 cttaataatg tagctcgctc agccaggtga gaggataaat tgagagactg gcttaaatga
    41761 tccatcattg cctttattcg ttgtacctta tcatacatgc ttcccagctt tacttgaaaa
    41821 ataacctgct cggtagaagg tatatgagcg cttagcggct gttttttatc ttgtttaaag
    41881 aaaaaggcag catcacttaa tcttgctctc attaccttct catttcctaa aaccacttgt
    41941 tttggattgg agctggcgat atttgatacc gtaataaaat aaggtagcag ctcaccatgt
    42001 ttatttttta gtgcaaaaca cttctgatga gattgcatgg aagcaataag ggcttcagca
    42061 ggaacctcta aaaactcctg ttcgaaattt gcaatgagcg cttgaggcca ttcgactatt
    42121 gaggtgactt catcaaccaa ttcttcaggc ataaccactg aagcacctaa aggtgccgcc
    42181 aattcttgta cctgatggat aatggcttgt cgcctagtag caaaatcggc aattacaaaa
    42241 gcatctttca tctgatcttc atagcttgca gctgaattta ttcttatttc ctgcggatga
    42301 tgaaaacgat gtcccctgct atgacatcca cttgtaacgc ctaaaatttt atgattaagt
    42361 gtctcacttc cataaagcat cacggcccaa tggacaggtc gggcaaattc gtcgtctcct
    42421 tctccccatc tcatgggttt tgcgataggt aaagactcta aagactgact aaccaatcca
    42481 ggcaagagat cttttgtttt agtaccctca ctctgagttt caaaaacaat ccattcacct
    42541 ttgtcagttt gagttttggt gagcgcatct accgtaacac cacatgattt tgcaaatcct
    42601 aacaaagcag gagtcgggtt accttcacta tcgtatgctg ctattgcagc aggaccgcgt
    42661 cgcatgactt tttggctttc ctgttcagtt tgtaagtcat gaatcacaat agccaggcgt
    42721 cttggtgtag cgaatctctt gacttcacca taacttaatt gcgccttatc taatgcggct
    42781 aacaatcgag tagaaaactc atttgccaaa ggccaaacag caccagaagg cagctcttca
    42841 catcctaatt caaaaataaa atcattgacc attacacacc ttttgcatag ggaaacctaa
    42901 cgcctcgcgc gcttgatagt aagcttgagc tactgctctt gatagatgtc ttacacgtaa
    42961 tatatacctt tgccgctctg tcacagaaat tgctttacgt gcgtccagta agttaaaagt
    43021 gtgagaagct tttattacca tttcgtaagc aggaagaggt aattgcaaag ctatcaattt
    43081 ttgtgcctct ccttcataat aatcaaattg cttaaataat tcttcaacat tggcatgttc
    43141 aaaattataa gctgacatct ctacttcatt ttgatggaat acatccccat atgtcactat
    43201 accattagcc gttttacacc aaggcaaatc aaataagtta tctaccccta aaacaaacat
    43261 tgcgatacgt tccaaaccat aggttaattc accagtgatt ggtttacata caagtccacc
    43321 cacttgttga aaataagtaa actgagacac ttccattcca ttttgccaaa cctcccaacc
    43381 taagccccat gcgcctagag ttggtgactc ccaattgtct tcgacaaaac ggatatcatc
    43441 caataaggga tctactccaa gcgcgcgtaa cgaatccaag tatctgtctt gtatatccaa
    43501 tggcgacggt tttaatacca cctgaaattg ataataatgc tgaacccggt ttggattttc
    43561 tccataacgg ccatccgtcg gccttctgga tggctgaaca taagctgctg accatggctc
    43621 aggtccaatc gctcttaaaa aagtagctgg atgaaaggta cccgcgccaa cttccagatc
    43681 caagggttgc aaaataacac aaccaagatc tgaccaatat ttttgcaagg tcagtataat
    43741 ttcctgaaaa gtagtaggtt tagtcatgaa aatctcaaaa aacaaatatg atctttaaat
    43801 gtaggttggt ttttaatcct tttatcgagg atatatccca taaaacctat ctttcattta
    43861 gagaattaat tattcagtcc aataaattat tggattgttg ggtgccaggg ttgattgaac
    43921 tcaacgccac aacactaaaa tactatccca gttgaggaca actgggacat atgaatttaa
    43981 tccaagtatt tagataaata cttaattgcc agccgccttt ttaattaact cacgtagtga
    44041 ttgctcgctt gtagcgcctg gtataaatga tatttcactg ccctttttat attgcccatc
    44101 tggtgttgag ccaattataa aggctggtgt acccatcaaa tgtaattttt cagccagttg
    44161 gcgattcgca tccaggatat cggtcacttc ctgactatcc atatcttttt tcagtttttg
    44221 catatccaga cctatggatt tggcggcatc catcactgtt ttttcatcca aacgtttgtc
    44281 tatcgtaatc aaagcattat gcatagcctg atattttcct tgcatgccag ccgccagagc
    44341 aactctggaa gccagatcag aagtcttgcc aaagattgga aattctttgt atataactcg
    44401 taatccgctg tcttttttaa ccagattttc aatggtagat gccatttttt tgcaatgaat
    44461 gcattgataa tcaaaaaact caaccagtgt tacattccct ttaggattac ctacagtagt
    44521 taatttgcct tgaaaaacct gttcagcatt ttcctgaatt gcagcttgag cttgctgttg
    44581 catattttgc tgctgttttt gttgtaatgc ttgcgaagcc tccaataaaa cttccgggtt
    44641 attaatcaga taatcatgaa tgactttttc aatttccttt ttttgggcat ctgataaaga
    44701 agcgctagcc gtatcagccg ccattattgc aggtgatact aatgtagagg ctaatgctcc
    44761 agctgttaat aaacttgtaa atttcacgta attcccctta cttataatta atcaatgacg
    44821 caaattacat ctcgcagata actttatcat taattgatct gctcaaaaca gcaaagtata
    44881 acaaatttct gttgcctaac gcaccctagc attcatgaaa aaaaaattca atattacaac
    44941 aacaaactgc aagtcagggt acctcagata ttttataaaa attaaatgca ataactacag
    45001 gcattcagtc aaattagcat aggccaaaac caaccatttt acaccatgtt cactgaattt
    45061 tacttgaact cttgtatggg ccccgctgcc ctcaatggca agaacgacac cctgtccaaa
    45121 tttggcatgc tgcacatttt gcccaattgt aatgcccgac acctcgtcag ccactggcaa
    45181 tttgcttttt gtagttccgg gccactgaga acgtgattta actcgtacct catcaagaaa
    45241 ttgttgagga atttcacgca aaaatcttga tgatctgtga tactcttcac ggccatattg
    45301 tcttctgacc tcagcatagg ataaaaccaa tttacgcatg gcacgagtca taccgacata
    45361 acataatcga cgctcttcct ccaagcgacc aggctcttca atcgattgtc tgcctggaaa
    45421 tattccttct tccataccaa ccaaaaacac aattggaaac tcaagccctt tggctgcatg
    45481 taaggtcatc aagtgaacgg atcgttcatg ttcatcagcc tgcaattccc ccgattctaa
    45541 tgaagcatga gcaaggaatg aattgaccaa cggcaactcc tcctcttcat cgtactcata
    45601 tcgaaattgt ttggcagcat taattaattc ttgcaggtta tccacacgtg attctgattt
    45661 atcacctttg attttgctaa agtgggcgta taaaccacta tgctgtatta catcagagat
    45721 ctgctcatcc aactccttat tagcaaccac aacctgtaat ttttcgatca aatcaataaa
    45781 cttggctaat gccaagctcc cccgttgccc taatcctgtg gattgaagta tgcccttgga
    45841 agcgtcccac aacgaacact gttctgctct ggcgtactgg cgaacttcat ccaatgtttt
    45901 ttctcctatt cctcgagtag gaaaattaac cactctttca aacgcagtat cgtcatttgg
    45961 attaactaac aatcgtaagt aagccaaagt atctttaatt tctgccctgt caaagaaacg
    46021 gactcctcca tatatcctgt aggctatacc tacacgtaac aaagcttctt ccaaaacacg
    46081 ggactgggca ttcgaacgat acaacactgc aatctcatcc gcactggcgc cctgattcaa
    46141 ttccatgcca attcgttcgg taacaaaacg tgcctcatcc aattcattga atgcactgta
    46201 tacaagaatt ttttctcctt cactaccagc agtccataac tctttaccca tacgagtact
    46261 gttgttgtga atcaatgcat ttgctgcatt taatatcatt gcagttgacc tgtaattttg
    46321 ttcaagtctt atgatttggg tatccttaaa atcatgaaca aattgttgaa tattttccac
    46381 tttggcccca cgccaaccat aaattgactg atcatcatct cctacagcca ataccgctgt
    46441 atgatcacca gcaagcaaac ggatccatgc atattgaatc gtattggtat cctgaaattc
    46501 atcaactaaa atagcttgga agcgttcacg gtaatgagca agaatctctt cattatctcg
    46561 tagcaattca tgagttcgta ataatagttc agcgaaatcg attacccctg aggtttggca
    46621 aacgtcttca tacgctttat aaattgaaac caaagtcttg gtcgggccat aatgcagtgc
    46681 attaatatgc tgtgggcgca agccttcatc ttttttacta ttaataaatg cttgcgcttg
    46741 tttgacttgc cattgctcag gatcaagatt aagtgaaaga atgactcgct tgataactcg
    46801 tgcctggtct tccgaatcca gaatatgaaa ctgttctgga aggttagcct ccttgtagtg
    46861 gcgacgcaat aaacgatggc ataatccatg aaaagtacca acccataaac ccaacgttgg
    46921 tgtagacaac atactgctaa gtctcgatct catttcaccg gccgctttat tcgtaaatgt
    46981 tactgctaat atagcgtggg gagagagatg ctgttcttca attaaccagg caattcggct
    47041 caccaagact tttgttttac cactgcctgc acctgctaaa accagcatat tacctaatgg
    47101 agaggttacc gcgtcacgtt gcttttcgtt taatcccttg agaagtgctg ctatggtcat
    47161 agaatatttc caacagaaaa agtgcaagta ttatcttcaa tttaattgct aaagcaaaga
    47221 tataatcgat acggtaaaaa atctaaaaat tctcctggat atatatcgat aaaacccata
    47281 caagtattgc tttctgatca atcagagcgc aaaatactgt tcgtttaaag tagctactag
    47341 atagtattat aatacaatga cgttatcata aaggactaaa atgcataccc tctacccagc
    47401 aattaaacct tacacacaac acgaattgaa ggttagtaaa ctgcatacac tttatttgga
    47461 agaaaccgga aatcctgaag gcatacctgt tattgtattg caccatggcc caggtggaga
    47521 agctgacccg catctaagac gtttttttga tcctcaacga tatcgcatta ttctttttga
    47581 tcagcgaggt tgtggccgtt caattccaca tttggaaata agtgaaaata atacccagct
    47641 tctcatggag gatatcgact ccattagaga tttcctgggc ttaagggaat ttgtgttatt
    47701 tgggggtgga tggggttctc tgttagcatt attgtatgcg caaaaatttc ctcaacaggt
    47761 gaaagcatta ctactccacc aaattttttt aggtcgacaa aaagacatcg attggtttta
    47821 taagcatgga gccagtctgg tttatcctga ttactgggaa gagtttacga acacagtccc
    47881 cgaaaacaaa cttgataata tccccaaata ttatgcggaa tgtctacaag gctctaatga
    47941 gctagcgcga atggctgctg cgaaaaactg ggctctttgg caagccagat gcagtagttt
    48001 acagccacac ttatatgtta ttgatcagta tagcgaccca catttcgcat tagcattagc
    48061 aactctggaa tcctattaca ttattaatca ttattttatt gaagaaaatc aggttatagc
    48121 taatgcccat aaaataaggc acattcctac ctatattgtt catggccgtt ttgacttggt
    48181 aagcccttta tccagcgcat gggaacttca tcaagcgatc cccgcttcca atctaagaat
    48241 tgtcagagat gccggtcact ctgatagaga atcgggaatt attgatgcac ttatttatgc
    48301 cagtaaggac atctcaaagc agggtcttga tgcatgttaa ctgtattaca aagagtaaag
    48361 gaagccaggg ttgatattga tggtcaaaca gttggcaaaa taaatcatgg cttgctgata
    48421 ctttgtggtt ttgaacctaa ggattcgcta gaaaatatta aacgaatgct ggataaatgc
    48481 attaattatc gcatttttga agacccgagt ggcaaaatga acttgagttt aaaggatgta
    48541 aatggcggtt tgctattagt accacaattt actctcatgg cagataccca aaaaggattg
    48601 cgtccaagtt tttcaaatgc agcttctcca gaactcgggc gtgagctttt tgataactta
    48661 ttaactcttg ctcagaaatg ccaccaaaac actcaaagtg gttgttttgg agcgaacatg
    48721 caagtttatt tatgcaatga tgggcctgtg acatttttgt tacaatttta atcagctttt
    48781 acggtattaa atttttgtat aacctggatt gtcgagcgaa gtataatcaa cattcattat
    48841 tgggattcat tgcgaccaaa aaaaaagcat gtttgtttca gttaaaaagc agcaatttaa
    48901 aaggtctatt ctttacatca aagcatttgt ttgcaagaac caaaaactgg tgtaacatca
    48961 ttttaagtat ttaataaata gaataattat gcgactaatc ttaatgtttg ctagcatcat
    49021 gggatccgtt attttagcga gctgtgcaag taaaggatca aacccgatcg atccattcga
    49081 accctttaat cgtaaagtgc acaattttaa tatggcattt gatgctaccg tattaagacc
    49141 accagcaaaa ttatatcagg ctgtcgtacc aaaattggta agaaaaggtg tgaataatgc
    49201 attcaataat ctggatttgt tccctacaat agccagcgat atactgcaag ctgaaggaaa
    49261 atgggtaatc aaagacagtt ggcgactctt cattaactcc actattgggg ttgctggtct
    49321 gtttgatgta gcagaaaaat ttggtttgcc accgcattac aacgatctgg gcctaacact
    49381 cgccaaatgg ggcgataaaa aatcgcctta ccttgtaata ccctttttag gccccagtac
    49441 tcttcgtgat ggagccggct ggctgttcca gtttgcactc tggagtcctt atgtatatat
    49501 tgatgatgct ggtctggttt atggtttaat tggattgcgc tacattgatt tacgaacaca
    49561 atttttcgac tctgagcctt tactgaatga agcattagac aaatacacat tcatcagaga
    49621 cgcttacctg caacatagaa attatcttat tgcagggact gagcaagata caggcacgtc
    49681 cttcgttgag gataagaaag ctaacaaaac tgcaatgcaa gaatcaaccg cttcaaataa
    49741 ccaactaggt tctgactatg ttgatgaata aaatgattaa ttgagacttg ccaggtcatg
    49801 ataagggttg ctgttgagcc aaatgcaaaa ccaaccctct ttaagctctg aacttcaaaa
    49861 tcaaaataat gactgttctt tattttgata aagaagtatg taacttttca aggcagcgca
    49921 tatattcacc tgcgatatcc aaaccagttt cagtgctaat ttcccgaata caagtgggac
    49981 tagtcacatt aatctctgtt agataatcac ctatcacgtc gatacccaca aaatacagac
    50041 ctttttctct caaagctgga gctatttgtt cacatatcca tttgtctctt tcagtaatag
    50101 ctaccacttc tcccctggct cctgcggcca aatttccccg caaatctcct tttgcaggaa
    50161 tgcgagccaa agcaaaagga acgggttcgc cattgatcaa taaaatacgt ttgtctccgc
    50221 ttactgtaat atccgggata taacgctgag ccataataca ttttgtctgc cctttggtta
    50281 atacctccaa aatgaccgat aaatttttac cctctttgtc aacataaaaa atggaattac
    50341 ctcccattcc ttcaagaggt ttaaaaataa cttgttgatg aatttgccag aactctttta
    50401 attgccgagc atctctggat acaaaagtag ggggacagca atgaggaaaa tttagggtaa
    50461 aaaatttctc attcgcgtca cgtaaacttt gcggtttatt tgcaaccaac accccttctt
    50521 gttccgctaa atccaaagcg taagttgcat aaatatactc catatcaaac ggaggatcct
    50581 tacgcatcaa aataacatca aaatctctca atggcttatt acccaaatct atggttttaa
    50641 cccaactcag actctctata tctccaatgt gaatttcatg gactcgtgca taagcgctcc
    50701 catcacaaca atataagtca ttctgggtaa agtaggcgca agaccatccc aatccttgtg
    50761 cgcttttaat catagctact gtagagtctt tatagggttt tatttgatcc aatggatcca
    50821 taagcaccgc aagtttcatc attcgcctcc aattgcagca atttctcgag ctgcagccag
    50881 tgcagccaag cgagctataa ccccataaac ataaaaacga tttgggcagt caactacctt
    50941 taaatcatca catggcatat tacaagcctg tgcgaaagct aacggctcaa aatgcatgcc
    51001 tggagcattg aggttctcat caattcctct gcctttatga acgcgataaa atccaccaac
    51061 aacaaattga ccaatcatat aaactacagg ttcagcaaca gaaccatcag gcatcgtttc
    51121 aaaagtataa acaccttcct gaataatgac tctgtctacc ttcctgcttc ctttactggt
    51181 agacatcctg gtacgctgct ttctattcaa ttgtcgaatt tcatctccat tatgaaccat
    51241 cataacgctc atgccataag taccattatc tgcctttact actgcgaacg gtttattttc
    51301 tattccgtaa gtagtgtatt tatcgtttat taacgataat atcttatcga cttcttgagc
    51361 taagatttca atcccttcct gggccataaa atcgaccccc tctatagcgc taaaataggg
    51421 attaattaac cacggatcaa taccgactaa ttcagcaaac tcaatagcta cttcttcaaa
    51481 aaattgaaaa tgactcgatt ttaatcgaga agcccatcct aatttggctg taggtctaat
    51541 tcgctgttgc acatcctgca atatttcagg aacgcctgaa gacaaatcgt tattcaataa
    51601 caaaaggcaa gggctgaaat tatccagtcc caccttatca ccttgtcttt gtaatggttc
    51661 aattaataaa gtttcacctt gttcgagtat tatttctgta ggctctttaa tgtctggatc
    51721 taaactgcct attcgaacta taaaaccagc ttttacaaaa attgttttta atacattcaa
    51781 actctgtaaa taatacttat tccgagtatg gctttctgga agaagcaaaa ttttagtaca
    51841 atcagggatg tactctatca aggcagattg agcagcttgt atacacaggg gaaggaactc
    51901 cgggtttaaa ttattaaaac cagctggaaa taaattggta tcgacgggag ctaatttaaa
    51961 gccagcatgt cgcaaatcaa cagatgacgt tagaggtgcc ggtgtttcct gccacttttt
    52021 tctgaaccag gattctattt cagcaagttt atttaatata attttttcca catgatacaa
    52081 aggacctgaa tgagcagtag tcagatgtgg aacggggatt tcatgcttat tcattcatac
    52141 tccctatttt ttaaaatgga tcgatgttac ctaggttatt cataaaataa aagcatttat
    52201 cattctt
//
LOCUS       DAXLDT010000005        50675 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXLDT010000005 DAXLDT010000000
VERSION     DAXLDT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 50675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50675
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.005"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            224..1651
                     /locus_tag="ACF2SP_000223"
     CDS             224..1651
                     /locus_tag="ACF2SP_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HFD2358039.1"
                     /translation="MKFLKKTISQWDTINLAICGLFSLLLIIWSFDQYLANDSYYYWR
                     WSQNLDWSYYDGPPLIAYFIRFCTTIFSDSNLTLALINKFMLLMTCIVIYTTARLNLN
                     KSASIISAMVCLFFPLSISSICRGTTYDVPQLFFYACSLYCATLFAKDINRYKNLYAT
                     AAAIGLLMLSKYSGVILVLGLLCSIIRQKPLRGLFFNPHFYLGVLLSLIIFSPVLYWN
                     FKHDWVSFLYQLDTHSLRHEVQNVNFVKLENVVEYLCTLNFLILIIWQLRPQNIAQAP
                     LGIRLQSDVTLVFFWFIIYLCFISKIHMNWVTPMVVSLSLLCGYAYQTGQLKKGICFY
                     LSFFIILDTAYVAYHIFYQKSDIKHEIAQYLRGLPYHNSSNEFLIGDGWTVATALYPA
                     KGHPLPKTLSCSNQPDNQYQYWNNSYLREIQSNPDAQVWYFSTSHALSCLNKYFNNCH
                     YFSKVLFSNRHKTFLRTYVCGNEPS"
     gene            complement(1864..3258)
                     /gene="fumC"
                     /locus_tag="ACF2SP_000224"
     CDS             complement(1864..3258)
                     /gene="fumC"
                     /locus_tag="ACF2SP_000224"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFD2358040.1"
                     /translation="MAKTRVETDSMGEIEVPSDKYWGAQTERSLHHFNIGKDIMPREV
                     THAFGILKKAAALTNLELGKLPKDKADLIVQAAEEVSKGLLDEHFPLHVWQTGSGTQS
                     NMNANEVISNRAIELAGGTLGSKSPIHPNDHVNMSQSSNDTFPTAMHIAAAIAFNEKL
                     LPAVRNLRQVFAAKMDAFKNIIKIGRTHLQDAVPLTLGQEFSGYVAQLDACIHRLEEV
                     LPELYELAAGGTAVGTGLNTHPQFAVKVAEHIAKITKLPFVTAPNKFAALASHEPLVH
                     AHGAMKTLACALMKIANDIRWLASGPRCGIGELIIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDTAVGIADSQGNFELNVFKPVIIFNVLHSLNLLADSCHSFQEFCAEGIE
                     PNQPVIDYYLHHSLMLVTALNQHIGYDKAAKIAKTAHHDNISLQEAAVKLGILTAEQF
                     AAFVKPEKMISPEQ"
     gene            3503..4237
                     /locus_tag="ACF2SP_000225"
     CDS             3503..4237
                     /locus_tag="ACF2SP_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2936 family Dot/Icm T4SS effector
                     methyltransferase"
                     /protein_id="HFD2358041.1"
                     /translation="MVVRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTL
                     FNGDNQEFTAFIERVKKKQVFVRIASVLEVNRESPLKIHLAQAISKGERMEVVMQKSA
                     ELGVACITPIITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQ
                     FVREAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLS
                     LGPRVLRTETAAITALSVLQAAGGDL"
     gene            4450..4776
                     /gene="trxA"
                     /locus_tag="ACF2SP_000226"
     CDS             4450..4776
                     /gene="trxA"
                     /locus_tag="ACF2SP_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFD2358042.1"
                     /translation="MSDHIKTVTDASFEQDVINSSKPVLVDFWAEWCGPCRALTPILE
                     EVAATHSKDMTFAKINIDENPQAPAKFGVMSIPTLILFKNGQVEAVKMGLLSKSQLSA
                     FVESNL"
     gene            5099..6361
                     /gene="rho"
                     /locus_tag="ACF2SP_000227"
     CDS             5099..6361
                     /gene="rho"
                     /locus_tag="ACF2SP_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFD2358043.1"
                     /translation="MNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHAL
                     KGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIR
                     PPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTAR
                     VVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPANRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEE
                     FKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDE
                     CDAIEFLLERMKNHKTNAEFFDAMKRQE"
     gene            6507..7973
                     /gene="ubiD"
                     /locus_tag="ACF2SP_000228"
     CDS             6507..7973
                     /gene="ubiD"
                     /locus_tag="ACF2SP_000228"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFD2358044.1"
                     /translation="MKYSDLRDFIAQLESRELLKRIDYPVSPHLEMTVVSDKVLRSGG
                     PALLFTNTPNYNMPVLTNLFGTVERVALGMGEESIVALREIGKLLAALKEPDPPKGFK
                     DAFSKLPLLKQALNMAPKYVSGAECQTHVWEKDEVDLTLLPIQTCWPGDVAPLITWGL
                     VTTRGPHQSRENMGIYRQQLLSKNKLIMRWLSHRGGALDYQAWQQEYPQERFPVAVTL
                     GADPATILAAVTPVPDTLSEYAFAGLLRGQRTRLTRCIGNDLHVPASAEIVLEGYLEP
                     GNEAPEGPYGDHTGYYNEVQSFPVFTVERITHRDKPIYHSTYTGRPPDEPAILGVALN
                     EVFIPLLQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYPGHAKRIMMAVWSFLRQFMYT
                     KFVIVCDDDVDARNWQDVIWAMTTRMDPSRDTVMVENTPIDYLDFASPVSGLGSKMGM
                     DATSKWPGETQREWGKPITMDEDVLNRVNSYWSLLGLK"
     gene            7973..8686
                     /locus_tag="ACF2SP_000229"
     CDS             7973..8686
                     /locus_tag="ACF2SP_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFD2358045.1"
                     /translation="MTDKIIQAQVEDISPLTDSIVRLVLMPDEYVDYQAGQYLQILFA
                     NDAFSYSIANAPLGSHRYELHIRHSQDNDYHQSLLAHIKQYGSLRLRLPLGECSIDQL
                     HPQKPILFIAGGTGFAPIKAMIEQLLADSDSRPFELFWGARSQSDLYMDEKVRSWGSH
                     ASHFQYFSLLSDKSEDTLASRVLARHANILRDWQIVISGPFDMVYSTRDTLVGYGISP
                     TQMFSDAFSFESKTDDAKN"
     gene            8704..8898
                     /locus_tag="ACF2SP_000230"
     CDS             8704..8898
                     /locus_tag="ACF2SP_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358046.1"
                     /translation="METLYQILGLIGAGLIIFILYRFIKGSPEQFSKENISKSFMTMG
                     VLGLILIGFIALLVLMLRNT"
     gene            complement(8936..9247)
                     /gene="hspQ"
                     /locus_tag="ACF2SP_000231"
     CDS             complement(8936..9247)
                     /gene="hspQ"
                     /locus_tag="ACF2SP_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFD2358047.1"
                     /translation="MNKIAKYNIGDLVIHKRIRYRAIVIDVDPIFQASGKYNPRATQH
                     EFATRNPWYRLLVDDSSQITYVEECQLLPDTSQEPINNPNIDDYLIKNKGSYQKAIPS
                     H"
     gene            complement(9309..10034)
                     /gene="tatC"
                     /locus_tag="ACF2SP_000232"
     CDS             complement(9309..10034)
                     /gene="tatC"
                     /locus_tag="ACF2SP_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFD2358048.1"
                     /translation="MSFMLIHLIELRQRAIYTLLWFGALALLFFFISDQLFHLLVRPL
                     LRSLPNQEGLIATQITSSVFTPLKLAVDTALLLTAPFALLQLWRFMRPGLYKNEQEQI
                     RGTIILSLLLFVFGTLFCFYLVLPYMFQFFAQALPEGVRLMPDMAYAIDFITRMLLLF
                     GFSFQVPLICLVLVKTHFTTVETLIKIRPYVIVGAFIIGMLLTPPDVFSQVALALPLC
                     LLYEAGIILARIYGKSPAPTLIQ"
     gene            complement(10140..10541)
                     /gene="panD"
                     /locus_tag="ACF2SP_000233"
     CDS             complement(10140..10541)
                     /gene="panD"
                     /locus_tag="ACF2SP_000233"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFD2358049.1"
                     /translation="MAYRKMLKSKIHRACVTQADLDYEGSITISPELLKVANILPYEA
                     VNVWNITAGTRFETYAITGEKGSTDICVNGAAAHLVTPGDLVIIASFTQILEEDCAAH
                     EPTVVFVDQFNRLKEIRPERIGVKSRIPYPA"
     gene            10877..11647
                     /gene="rsmA"
                     /locus_tag="ACF2SP_000234"
     CDS             10877..11647
                     /gene="rsmA"
                     /locus_tag="ACF2SP_000234"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFD2358050.1"
                     /translation="MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGA
                     LTQPLLQKLNRLTAIEIDTDLQNYLTRLPVSQGKLNLIPADALTVDFCQFGPHLRVVG
                     NLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEV
                     EYLFDVPPEAFEPKPKVDSAIVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNN
                     LKGIISLSQLNDLGIDGGKRPEQISVAEYVQLAKFISN"
     gene            11684..13021
                     /locus_tag="ACF2SP_000235"
     CDS             11684..13021
                     /locus_tag="ACF2SP_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFD2358051.1"
                     /translation="MFHRQRKSSLSAQSKSLKDLIRMVTAEQFLSEEKRQVLLQKMRA
                     FSGLEASRYESLCSVLVENLVNYCQNLPETANSYYSQPGGLVDHALNRTEAALSLFKE
                     FTVQEQPDLLSEEQKLWQYALYSAAILQGLGKLFDEFRINLFDSNGQLLKQWNPLLES
                     LIQTGSYYDYQFEKEADAELRRRLNLLLARTLMPASGFAWIASNPEVLAVWLALLNED
                     YRSAGTLGAILIRADAIAIQRYFLAFMARSGARRSGRYGRAVTFTGGVPESITEKEQA
                     IGVEFIQWLIKSLDEGRIMVNKAPLFMVPGGMLMCPEMFQLFVREHPEYKNWQAIQNG
                     FLSLGLHGRDAGGNVTSRFEQAHTQQMYTGVVFAGYGVALPNSVQVHHLNTGKIESMS
                     ALELIHKSQYTSQFTQQSSAIAIAPLQRLAANGQWQNIEQDMSQLTFGAKRGA"
     gene            13014..13844
                     /locus_tag="ACF2SP_000236"
     CDS             13014..13844
                     /locus_tag="ACF2SP_000236"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFD2358052.1"
                     /translation="MPDYAIGDVQGCYDPLQRLLELIDFNEKEDCLWFVGDLVNRGPD
                     SLAVLRFIYSLPVKPKITLGNHDLHLLGLLFGGQPWKGHDDTLEEVMLADDGEELGHW
                     LRKQSLLCRSSELNIVMCHAGIAPLWDLSKAVGLANELEAVLSGDSYHEFFAQMYGNK
                     PDIWSDDLVGLDRLRVITNYFTRMRYCDAHGRLDLGYKGTLSKAPNHLYPWFEVPCRK
                     EIEMDIVFGHWAALMGRSSHPRIHAIDTGCLWGGQLTALRLQDRQRFSVPGYGVSRFE
                     "
     gene            13888..15516
                     /locus_tag="ACF2SP_000237"
     CDS             13888..15516
                     /locus_tag="ACF2SP_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2358053.1"
                     /translation="MKFLNKSTALLPVIILSGLISFHAASAKTSSNADNSLYSWTENP
                     SAEAQRVEQAEERLKAAIANPEYMLKNWIELPTSPESKKKTRHQLTIREAILLALRYN
                     PNIQNAELDRIIQRYQLRLANNEFELQYALGASGVVQKSTFEGIGSSTNHSFLASPEL
                     DLKTKIGTKASVSIDNNVSTNNSYNPVLNLSITQPLLRGFGKSANEAKLLDAIDKEWL
                     NKLSLQQSVMDQITQVITAYRALILSGNNLENQRLQLEEARKSYRINEKKIAAGQLEP
                     TGNIQQSYQIESLSLMVEQGENDFKLASQELLQMIGLDPDLHISVPSDVTIKEIIVPD
                     LQESIAIALSHNTQYLAQKMALRADERAYTVAKNQQLWQLDLAGTVQGGAVNDVTGTS
                     SGLSSIYNGNNITESARVTLTIPLNDIGRRSQLINAKVQLEKDKINLIAAKRALITSV
                     TNTINNIQSLAKRYELAQKQVKLAEQSYALEKKKQQAGISSALDVNNTQNQLIQAQSG
                     LIGAKIAYLNQLSALQRILGTTLDYWQIKLRYGG"
     gene            15513..16658
                     /locus_tag="ACF2SP_000238"
     CDS             15513..16658
                     /locus_tag="ACF2SP_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2358054.1"
                     /translation="MNNYLKIFSIVLLILMLVSCGSSDKPQTSLLKTYEVKPQPLHKT
                     LHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNELQKQYNDTLTD
                     YLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQATRKLSEMLE
                     KMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDKNNRVTV
                     GSTIKSGQVIALIGDLKGISVEIDVPEIDIDKIRPGMQATISGVAFGRHQLKGKLVAV
                     NAQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLLIPISAVK
                     REKGSSIVQVQLAKGKVEKRMITTGAAQADSVVVESGLKSGDVVVYD"
     gene            16651..17340
                     /locus_tag="ACF2SP_000239"
     CDS             16651..17340
                     /locus_tag="ACF2SP_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358055.1"
                     /translation="MIDRPLIQLSDLVKTYHLEGISTTVLKAVSLTVNEGDLLAIVGA
                     SGSGKSTLMNIIGLLDKPDAGTYTLNNRNVASLSDDEAADLRNQNIGFVFQQFNLLPR
                     FTAMQNVALPLTYRGVNPTLIKEKVEQALDKVGMRQYIRHRPTQLSGGQQQRVAIARA
                     LVTDPQVILADEPTGALDSRTGSEVMNLFLALHQEGRTIIMVTHDEHVAAQCKRRITL
                     ADGAIIAECGQ"
     gene            17337..18530
                     /locus_tag="ACF2SP_000240"
     CDS             17337..18530
                     /locus_tag="ACF2SP_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2358056.1"
                     /translation="MNFLNHCQQALVNLTASKLRSLLAVLGILVGTAAVVALISCGQL
                     ATEKALEQFKALGTDLLAVSVYQKVPSKSHSESDSLTIDQWQQIAERIPYVLRIAPYS
                     TAYQTLSFNGKVMQGAVIGADESLADIIHVTLAQGHFVSFLDSFEHFCVIGHSLAQQI
                     KETTLDDPIGKQLRIGQSLYTIIGVAERWKENGFFNEDINQAVIIPLAGMSLVSKDAK
                     INNAVLLLKPDSPIDEVINEIKEIINSQAPKLSVFPRSAKQIIASMENQGRIFTLLLA
                     VIGGISLLVGGIGVMNVMLVSVSERKKEIGIRKAVGAKNREIQALFLVESVMLSLLGG
                     VLGVILGLIFTRIVAYFSDWTFTVYLLPPIAGFLVSAATGIFFGFYPARRASKLEPMV
                     SLRSE"
     gene            18865..19827
                     /locus_tag="ACF2SP_000241"
     CDS             18865..19827
                     /locus_tag="ACF2SP_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HFD2358057.1"
                     /translation="MKKWGLAIVALMLFNQITYASQSWNQWVAGVREEALQQGIPPSL
                     FDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKRNKEILEE
                     IAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
                     LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANY
                     MKQNGWQAGEPWAIQVKLPAKFDMKLEGKGIVKPVSEWNALGVRTESGEPLPYQNLQA
                     SIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ"
     gene            20152..21048
                     /locus_tag="ACF2SP_000242"
     CDS             20152..21048
                     /locus_tag="ACF2SP_000242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358058.1"
                     /translation="MKPVFAKENGRWSTVYNSEKYQDVYFVGFRVKFDGSEKLQTLTI
                     KTDKNYSVALSYSIDGSSDVVCDEFSWTNALLQAANQDGYCAARPKEFELHTKESEYY
                     YALTEASKSAIWKDHKEAMDLFRHSFTAGTAFALPNTKPGPKFQNNASELQSTSSISI
                     DQELSPLSEEITKQVTFKEVSNNRTRKANAPHENSPQYSLIINPEVKPSQIKETHPSL
                     MSNISMMVIGGFIAAAGITAIAIAFTVLNAATFGVAGLLVAGLGVAATLSGAGLFATG
                     AYKHLHTIGEDSLGLSGNLVHQ"
     gene            21376..21903
                     /locus_tag="ACF2SP_000243"
     CDS             21376..21903
                     /locus_tag="ACF2SP_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358059.1"
                     /translation="MKIEIIPATISDYPIVQNLASYYVYDRTGYMGWQCSEDGIFECI
                     DFKHYFETPNEKAFLVKVNDELAGFVLLDKEHLLEAVDWNMGEFFILRKFQGKGIAPY
                     VAHKILKENPGKWSIAVMPENIKAVNFWRKIISALVMESYTEVFKTKDELKTPENPDA
                     YAMNIFTFNTEKCTN"
     gene            22293..22796
                     /locus_tag="ACF2SP_000244"
     CDS             22293..22796
                     /locus_tag="ACF2SP_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358060.1"
                     /translation="MTKILICLGLFLSSNLLMAKSLSQLDSANLLPCFNMEQAERIGK
                     QINKLLQHEFCEENINPKKFASISHNILPKIMTETFLGVTPPENWQQLSDDIIKNCIA
                     NKNLCKKAARKELEECIKPRIPLILIQFGPWLAQNCPQLNKSLIEQWPNKQATLKKII
                     NENKSAE"
     gene            23308..23592
                     /locus_tag="ACF2SP_000245"
     CDS             23308..23592
                     /locus_tag="ACF2SP_000245"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFD2358061.1"
                     /translation="MQTTHHDSKSDRIEARVSSEDKDLFKRAAELSGMNNFTAFVIKA
                     LKDASVKVIKEHALIELSLRDQQLFVDSLLKDTAPNQRLLKAAKRHKKLI"
     gene            23604..24107
                     /locus_tag="ACF2SP_000246"
     CDS             23604..24107
                     /locus_tag="ACF2SP_000246"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358062.1"
                     /translation="MNKYCIEQLNKSHVKDNFNSGEDSLNKYLQKQATQDIKRNLSAV
                     YVLLEGQDSTILGYYTLCSSSVLIDSIPHEVSKKLPRYPLIPVTLLGRLAIDIKLQGQ
                     GLGELLLTDALLRAGRLSNELGSMAVIVDSLSSRASDFYEKFGFQKFIDKTNKLFLPM
                     SIIKQLL"
     gene            24434..24547
                     /locus_tag="ACF2SP_000247"
                     /pseudo
     CDS             24434..24547
                     /locus_tag="ACF2SP_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(24695..24886)
                     /locus_tag="ACF2SP_000248"
     CDS             complement(24695..24886)
                     /locus_tag="ACF2SP_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358063.1"
                     /translation="MRNKDEKAKEVVTDSRCAEANRLTHLITGAILTDNCFFPSGAYS
                     IKPMVSNSQPPREKTTPSM"
     gene            25154..26047
                     /locus_tag="ACF2SP_000249"
     CDS             25154..26047
                     /locus_tag="ACF2SP_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MC/SLC25 family protein"
                     /protein_id="HFD2358064.1"
                     /translation="MKDKTIPGPDAKSFNKEEFSENVAKGAIVGATSTTMTFGLFDKP
                     LAIATKASSEVAQHPWRGIKNLFAGAINGNFSTSWNNSLYVQAYRAYLEHPVKGYPVA
                     LLNSCLKNVVFFPAKYGSERALYALSGNEQQAKNYSGFLAGLMTVYLTAPVSVVKARL
                     MTNVPLNTLSLKRLGSGMNAIAMRDCIQYGIYFNAKEWLNEKISDGVMAGGIAGIVGY
                     TFSNPFSVIGLNQKTAVESVNIPNMAKRIYQANGVKGFYPLVGLAALGMFARGVAIHQ
                     GTKLFESLQENEAYGSPTRKR"
     gene            complement(26432..27919)
                     /locus_tag="ACF2SP_000250"
     CDS             complement(26432..27919)
                     /locus_tag="ACF2SP_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2912 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358065.1"
                     /translation="MSYSKIDVLERKWSSLEAIVKSYTDPVEYHLPEIKKLMTAEFVV
                     DFSSHAIYLHTEYVHRFTQQLINLRNTSPNDERLLYIHQLWTDLLNCRNQNPLKIKHH
                     KKLDGECTFNFSLKMMEGKPYGFKEVQILDPNNTAELNKAMGEIKRIDSLAHGANFGD
                     LPFIGDIVLSSMLHYKETICFIAKDKNDEVIGYCWGMMLRDIPAGEKNKVNVFWVMNL
                     AKHPDFYDPNIKVGEALRQHLANTLSKNPDCHFLGYEHVLNHNFHLAIVDGGIPKKEE
                     TISIGQNQYQAKSLVKYEPLKMQYDRKHIIKTNNHQYPYPKVDVIKSKFFTALWYAAP
                     TAFDFILGGILIYGRAYYQQWTHSMLGQPVTHRIEKPIPHEQQICDMNILKRIILSDV
                     WGQQGTTLFFDACIPDTIQKLQQLVKEDQIEFASLQQCVAKSGWALCRSKLTAYFYFV
                     ITQSQYPSAAINLLIQNDMTPKKWIELISEERQIFAQQSSERASI"
     gene            29159..31093
                     /locus_tag="ACF2SP_000251"
     CDS             29159..31093
                     /locus_tag="ACF2SP_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFD2358066.1"
                     /translation="MHVLDPLTLEEIQESCDILKREKELSDSYRFAWVMTYEPPQKEV
                     LHSSDTDFDRCAFLSVFNKKTNETFEAVVNLNSKKVVEWKQIVLEVPPYGKPPILIED
                     FQKCEQIVKADPTWRAAMEKRGLDDEQIDNIQVDAFSAGYFDEEEEREQRIVKGISFY
                     RDNLKDNGYARPIEGVVAIVDLGNEKVLRVIDDGRNVPIPKEKINYDSDSYPEKRKDL
                     KPLDIIQEEGPSFKVNGWEVNWQNWSFHVGFTPREGLVLHQIKYLDEGKERPIIYRAS
                     VTDMIVPYADPSVSHYWKSAFDAGEYGLGKLANELKLGCDCLGNIYYFDVPSADDFGN
                     ARLIKNAICMHEEDAGTLWKHYETRNSTFEVRRARELVVSFFTTIGNYDYGFYWRFGQ
                     DGSLKLEIKLTGIVQTAAIFPEAQYEWGGKLTPELAAPTHQHFFNARLHMMVDGEKNS
                     FSEHEFYPIAMGGKNPYGNAFGSSQKTFEHEGEAARSACAQTQRTWKIFNPNSVNGIG
                     TAPAYKLELPETPLLLADEESYIYKRGGFASKQVWVTQYDPQEKYAAGDYPNQSSGGC
                     GLPSYIKQNRKIDNDNLVLWVTLGPTHFPRPEDFPVMPTSIISAKLQPFGFFKRNPAM
                     DLPQGKVQEKGDTCCSSLTY"
     gene            complement(31141..31329)
                     /locus_tag="ACF2SP_000252"
     CDS             complement(31141..31329)
                     /locus_tag="ACF2SP_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HFD2358067.1"
                     /translation="MADIINLNKKRKAKVRLEKEIKASENRIKFGRTKKEKQQEKQDN
                     ERSERHLDGHKLDKKEEN"
     gene            31990..32859
                     /locus_tag="ACF2SP_000253"
     CDS             31990..32859
                     /locus_tag="ACF2SP_000253"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HFD2358068.1"
                     /translation="MSNYLDKTASLTPATKRIVCNKATEYPHTGSYNQVATHGTYLCR
                     RCGLALFRGVSQFSSGCGWPSFDDEIANAVARKPDADGQRTEILCARCDAHLGHVFTG
                     EYMTYKNLRHCVNSASLDFVADNEVLDTEEAILAGGCFWGVEYYLKRIPGVLKVESGY
                     TGGHVSEPSYEQVCSGTSGHYEAVRVVYNSAKTNYHDIVKRFFEIHDPTQANGQGPDL
                     GHQYLSAVFYFNQEQLQEAELLIQLLKKRGYQVVTQLLQAQPFWPAEDYHQDYYFKHH
                     KIPYCHKPVDRFG"
     gene            complement(32883..33848)
                     /locus_tag="ACF2SP_000254"
     CDS             complement(32883..33848)
                     /locus_tag="ACF2SP_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358069.1"
                     /translation="MAFSKSETTVDKFSLDEFPAFSAHDLEVAFSLKKKYRESKESGY
                     PFTGFVCDYERRDKLYDFIYSSCHSPKLFENNFKVQIAYLDREKSGVRHWSLIELQYS
                     IEKGNNTLKILVLDSLGAQLSYNTAQILANEVGFQDLLDENEDLSIKIYIPDVKLQHS
                     RAGCATFTMDCASMLSTQDKYENIYDFMDKQAEVSDKVKIAKLDADFPVHCASLPPRI
                     LRGAQSRAIITDDKVLNSTPLINSKEETFKHSLLKHCSFFEDQPQTPENIKRNERIMK
                     KALKWRNTIDLKMKTSSAEEFENTCAIDDLEEYANSCLPSKSVQL"
     gene            34028..34288
                     /locus_tag="ACF2SP_000255"
     CDS             34028..34288
                     /locus_tag="ACF2SP_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358070.1"
                     /translation="MKILVKSSILAIVLALSTPLFADDTQWMTEHNLVKGVITFQDDS
                     QIQVYAAQADLDKLQQNLANIQSVEVQPNQTDSTTSPNQQSE"
     gene            complement(34331..34600)
                     /locus_tag="ACF2SP_000256"
     CDS             complement(34331..34600)
                     /locus_tag="ACF2SP_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HFD2358071.1"
                     /translation="MSLGELLLIFIVAIIVFGPSKLPMLATHLGMLVRKINQLKEHAS
                     SFWQQQLNEIQLYENQRKAEKADQQYQQTDSENQNSDYKKNTRPS"
     gene            complement(34597..34782)
                     /gene="tatA"
                     /locus_tag="ACF2SP_000257"
     CDS             complement(34597..34782)
                     /gene="tatA"
                     /locus_tag="ACF2SP_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HFD2358072.1"
                     /translation="MGLSGISPLSLLLILAIIVALFGTSKLKTIGSDLGEAIKNFRKA
                     MNSEETNDTQKDDHKPS"
     gene            complement(34784..36433)
                     /gene="ubiB"
                     /locus_tag="ACF2SP_000258"
     CDS             complement(34784..36433)
                     /gene="ubiB"
                     /locus_tag="ACF2SP_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFD2358073.1"
                     /translation="MKSIKQLIRLIHINYILAKNGLDNVVVSIKLFAPLRFIVYLNPW
                     NWLRKEKLTRGEALRKSLEELGPIFIKFGQALSTRPDILPEDIAKELSKLQDKVPPFP
                     SHIAMNIIEQAYKQSAYDVFAQFDPIALASASMAQVHAATLKTGENVVVKILRPNMRR
                     LIEQDLSIMYTIAALTDRYWPEGKRFKPKEIVKEFEHTLLDELDLMREAANAAQLRRN
                     FNQSPMLYIPEIYWDYCHHNILVIERIHGIPVTDIVSLRDHGIDIKKLAERGVEIFFT
                     QVFRDCFFHADMHPGNIFVSYQNPKDPQYICVDFGIIGTLTDNDKRYLAENLVAFFNR
                     DYKRVAELHVESGWVSRDTPVAEFESSIRTVCEPIFEKPLKDISFGQVVFRLFQVARR
                     FHMEVQPQLILLQKTLLAIEGLGRQLYPELDLWATAKPFLEKWVREQMGPKAFIKRLK
                     QNLPFFTEQLPHMPKLIFDILEMKKDQLLTVHEFTRLQSENNNQTIQWKSVGLGVFFS
                     FLSLGVISYFDLLDYNHLTTITIAGSVLAGIFVLINRKIRN"
     gene            complement(36523..37140)
                     /locus_tag="ACF2SP_000259"
     CDS             complement(36523..37140)
                     /locus_tag="ACF2SP_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HFD2358074.1"
                     /translation="MLKEYSLKALQTAINQAMKLDEQMPQKIQKLDGKTLEMVITPLN
                     VNFYIRFKGSEMQLLHRIDGRPDTIIHSNPIGLIRLSLLPTSKARSLFNDKIRISGDI
                     ELGQSVKKLFDEIDIDWEGHLAHFTGDVVAHQIGSFVRKGLEFKNQFSTSMQQNITEF
                     LQEELRISPSRNELEDFFAEVDELVLSVDRLQAHINNLMSNDEGN"
     gene            complement(37148..37900)
                     /gene="ubiE"
                     /locus_tag="ACF2SP_000260"
     CDS             complement(37148..37900)
                     /gene="ubiE"
                     /locus_tag="ACF2SP_000260"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFD2358075.1"
                     /translation="MTNQKQTTHFGFKSVDWNEKEKKVAEVFHSVAKNYDRMNDLMSL
                     GIHHLWKRYTIELSHVRPGQSVLDLAGGSGDLTRLLSQKVGDSGQVVLADINAAMLHV
                     GRDRLLDEGLFKNIRYVQGNAQCLPFADNSFHCITMGFGLRNVTDKDEALQSMYRVCK
                     PGGKLMVLEFSTPVFPGLKPVYDWYSFNILPKIGKFVANDEASYQYLAESIRMHPDQE
                     TLKAMIERVGFEDCHYHNLSGGIVALHIAYKY"
     gene            complement(38025..38450)
                     /locus_tag="ACF2SP_000261"
     CDS             complement(38025..38450)
                     /locus_tag="ACF2SP_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFD2358076.1"
                     /translation="MFYGDTVQETRQLFFISWNKYQNKKELSPLEKEIAQVILDHPEY
                     HKVIEQIQSFPEHTYYPELGETNPFLHMGLHLAVREQVTTDRPQGIRTVYTKLIEKYQ
                     DPMAVEHLIMDQLAECLWLSQKNNRPPDENHYLITLQNL"
     gene            complement(38751..39701)
                     /locus_tag="ACF2SP_000262"
     CDS             complement(38751..39701)
                     /locus_tag="ACF2SP_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358077.1"
                     /translation="MIEFLGIRFTGRPNVAAIVNFGKKRPIVNICLAALSLLLVASPT
                     GAMSQKASNMSVLNSLFINPGNSMSKSLAVPSPSCFSRQACITDANFQQEFTGFVNFI
                     HSRYPSAQVPSAERLQRHACNTLSPGGSHSTDDLGASNLEGMKMGQWLATYLSYSFWS
                     TQPGMNPYPTSPVWWCFLDNSSRYVVTDTIEVNGATNIIANFDGLGKPAANGGAGFYV
                     SQAIVTDAYPAISLEPGFYAAQTYTNKELVAIDLAAPGTTKPLAQYQSLMKIQAHETY
                     PRRTISLIQETINRLATIEWLGLCSIACTNCAPGTICPQS"
     gene            40222..41274
                     /locus_tag="ACF2SP_000263"
     CDS             40222..41274
                     /locus_tag="ACF2SP_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358078.1"
                     /translation="MMKTFIIAADRTVDSFGSHQDTDNILNLAREKGLVVVTLEILSL
                     AQGWQDKMKPHQFKSGASAMAAIERARKILSTKKADAVLIKGQDFLKTGYGSCEREKF
                     MKFYHENYTPLQGYNELVPLFLQKYKLDEKEYFKIRDALFENYTKTWKKINPANSMPH
                     EKWYQPLTKYFRGVDCANPSVDYSGQIILSTEKVADVLNIPQKARVEILGNSFVKINL
                     KGLASLPKIAPYHHLKRAINKAITQAKLNFKDKFMNEKALLDAYTCFPIVPMALLYCL
                     GLVNSLTEIPDLLKSYEVTVTGGLNLGKAPWSLTSLNALIVMRERLISSDKAKFGLVH
                     GNGLFGNQQGITIIKK"
     gene            complement(41459..42064)
                     /locus_tag="ACF2SP_000264"
     CDS             complement(41459..42064)
                     /locus_tag="ACF2SP_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HFD2358079.1"
                     /translation="MLIYFNGLLLGLSLIMALGPQNVFLIRQGARKNHAALSAVICFF
                     CDVILVCGSVAGLHQLLLAHPNLQIWMVWLGSAFLIYYAIKNLKSALSKNKQTVVETH
                     QPHTRMQIILFALGFSLLNPHAIIDTLVIVGSGSSQYPDHKLAFLAGVISASMIWFSS
                     LTMTTRYFSNLLTQNKVWQKIELLSGILMAYIAMKLITSSL"
     gene            complement(42123..43151)
                     /locus_tag="ACF2SP_000265"
     CDS             complement(42123..43151)
                     /locus_tag="ACF2SP_000265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD2358080.1"
                     /translation="MKIFNAMFSKVNGGLEQVFLNYIPALSSQGNLVIPIIHPKAEIR
                     NSCPQDHLITVHNFNQHDFFAIHRLKKLIKIHQPDCIITHSYRAAYLFKKTGTKVPKI
                     AVCHVKGHYDFGTDAIIALTEQMRRDIINSGIEEHRVFTIPNLIHIPDSLQYREPKNN
                     QIPTIGVCARFAAIKGVDVFINALAELKKRGVAFQAKIAGDGKEKEHYIELIRHHGLD
                     HEISLLGWIEDKESFYKSIDIFCLPSREESFGLVVLESMMHSLPMVLTRLSGPLEIAG
                     NSGCALFVPPEDPVSMADGLERLLRDKDLSKQLALKAFQRVQHYSSTKVAPMLHKVLE
                     KICDTNQQ"
     gene            complement(43217..43570)
                     /locus_tag="ACF2SP_000266"
     CDS             complement(43217..43570)
                     /locus_tag="ACF2SP_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HFD2358081.1"
                     /translation="MSFKPAVELVKLQQNNREVAVIDGQKILIIWHDNQVHAIQSQCP
                     HLKLPLAKGKITESCSIICPFHKSEFDLKTGEAKCWSPWPPVVGNLLGKISSRKNLKI
                     YPTQIENGQVLVDVG"
     gene            43952..45565
                     /locus_tag="ACF2SP_000267"
     CDS             43952..45565
                     /locus_tag="ACF2SP_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFD2358082.1"
                     /translation="MGSLVIKKSIIAALVVALGIAGQSVQAESVPDEKLQSEKVQTEK
                     VQAEKTETDLFSPVEQIMNPFYPKYPPTKPATGEQAELIKRGEYLAKMGDCIACHTDV
                     EGGTPAYAGGLPINTPFGLFYSPNITPDKETGIGNWTEKDFIRALKDGRDPKGRNYFP
                     VFPYVYFSKITDDDARALYAYFMSIPPVKLKNKSLPFPFGVPGARFALWGWNLLFFFP
                     QENSIEYDPDKSPEWNRGRYIVDTLGHCSMCHTPMNIFGSAKNRYYLTGSFIDGYWAP
                     NITKYGLESATHQEVADVFKAGELINKAGPVAGPMAEVNHNSLSHLTEADRLAIATYL
                     KTVESEEYLGVSPSDLPPTIARGRQVYLKACLICHQDNKMGAPVIGDSASWYTRLKSS
                     GLTGLYRHAIYGYNQMPVKGACVTCSDNDIISAVDYLLNASLTRSQWQDLASGGVKKY
                     PASGKAVYNENCAVCHNEGKLGAPKIGDKVTWKPLIAQNMDVLIENTIRGKNHLPNGG
                     CNHCSTDEVIEAIKYMVSQSKTEGNYSLW"
     gene            45647..46852
                     /gene="coxB"
                     /locus_tag="ACF2SP_000268"
     CDS             45647..46852
                     /gene="coxB"
                     /locus_tag="ACF2SP_000268"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HFD2358083.1"
                     /translation="MLNRLNICKLIALFSALTSAQSLWAAADEWQLNMYKGVTPLSND
                     MYYLHMVAMAVCAVIGVVVFGVMIYSLIHHRKSKGYQPASFHDNPKLEIIWTIIPFLI
                     LVGLAVPATRVLIRMDDTQLSDVTVKVVGYQWKWQYQYLDQGISYFSSLSTPYEQIEN
                     KQKKNQWYLLEVDNPLVVPVNKKIRFLVTSNDVVHSWWVPELGVKRDAIPGFMHEAWA
                     RIEKPGVYRGQCAELCGINHGFMPIVVKAVSEEEFDRWVSEQTKVEDIYAEKSDKPRE
                     QKTMTRDQLMTLGKQKYDLICSACHQPNGTGLPPMYPPLKGSSVAVGKPISRHIELLL
                     NGVPGSAMQSYKDQLTDEEIAAIVTYERNAWGNNTNDLIQPADVAALRHGTDIKPTMV
                     NKAKAGGLQ"
     gene            46849..48465
                     /gene="ctaD"
                     /locus_tag="ACF2SP_000269"
     CDS             46849..48465
                     /gene="ctaD"
                     /locus_tag="ACF2SP_000269"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HFD2358084.1"
                     /translation="MKYAIAHSEDSHEDHGPEQGKGFMGFVKRWLFTTNHKDIGTLYL
                     WLALISFFVAGAMALVIRLELFQPGHRFVDPNFFNQMTTLHGLVMLFGVVMPAFTGMA
                     NWQIPMMIGAPDMALPRLNNWSFWILPFAFCLLFSTMFHAGGGPNFGWTMYAPLSTTY
                     APPSTDFMIFSIHMMGLSSIMGSINIIATILNLRAPGMTLMKMPLFVWTWLITAFLLI
                     AIMPVLAGAVTMMLADRHFGTSFFNAAGGGDPILFQHVFWFFGHPEVYVLVLPAFGVI
                     SEIIPTFSRKPLFGYHFMVYATVSIAVLSFIVWAHHMFTTGIPLGAELFFMYTTMLIA
                     VPTGIKVFNWVSTMFKGAMTFETPMLFAIAFVFLFTIGGFTGLMLALVPADFQYQDSY
                     FVVAHFHYVLVPGVIFALVAATYYWLPKWTGHMYNERLGKWHFWLSVISVNLTFFPMH
                     FLGLAGMPRRIPDYALQFANFNMIVTVAAFAFGLSQLLFLYNVVATVRKRGTNKDVAT
                     SRVWEGSHGLEWTLPSPPPYHTFTTPPEIK"
     gene            48478..49020
                     /locus_tag="ACF2SP_000270"
     CDS             48478..49020
                     /locus_tag="ACF2SP_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HFD2358085.1"
                     /translation="MIEEKKQKKLLTILTLLVLGMFAFGFALVPIYNSLCKNLGINGK
                     TKMEAVAYDEDKAKIAADREVLVEFVATNNSGVPWAFYPKTKKIKVHPGEIVKLAFYA
                     ENKTDHTMTVQAIPSVTPGIAAKYLKKTECFCFTQQTLNGHEAMNMPLLFHLDTDLPK
                     NVNTVTLSYTLFDVTGRSSN"
     gene            49179..50048
                     /locus_tag="ACF2SP_000271"
     CDS             49179..50048
                     /locus_tag="ACF2SP_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HFD2358086.1"
                     /translation="MGAHGTYYIPKPSHWPLVGSIGLTTTLVGAACWLHHDWYGPYIF
                     IAGLCVLVAMMFGWFGQVIYENSKGLYDLQVDRSFRWGMCWFIFSEVCFFGAFFGALF
                     FARYWSVPLLGGEMHPITHYTLWPEFKAVWPLLSNPNNQTFAGAHEAMGAWGLAAINT
                     IILLTSGVTITWAHWALKLRKRGQLLTGMSLTIALGVLFLMLQSYEYHEAYTEMNLTL
                     DAGIYGTTFFMLTGFHGLHVTIGTIMLIVILVRCKRGHFTPERHFAFEGVAWYWHFVD
                     VVWLFLFVFVYWL"
     gene            complement(50071..50343)
                     /locus_tag="ACF2SP_000272"
     CDS             complement(50071..50343)
                     /locus_tag="ACF2SP_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358087.1"
                     /translation="MLIQPIEPVVITIKIGQNNFECQMISLSESELELKSNDYFEKES
                     QVTFIAKYFRGHAIVRELEFSDYCFTYKMEIVRIQFQPGLLINTRL"
BASE COUNT        14939 a         9570 c        10892 g        15274 t
ORIGIN      
        1 tatctttttt caagaaattt ggtattaaat ctttttgcca gactaaaata ttttcgggat
       61 ttcacaacac attttgaaaa attatatgaa aattataaag tcatgccgtt tgttgaatgc
      121 gtatttgttt gggttaaact gaaatccagg tatccctaga caaaagctgc taaaaagaat
      181 ataaagccgt gcacaatgaa tcacaaatta atttagtaat gccatgaaat ttttaaagaa
      241 aaccatctcg caatgggata ctataaactt ggcaatttgt ggtttatttt cattacttct
      301 tattatttgg tcttttgatc agtatttggc gaatgacagc tactattact ggcgttggag
      361 ccaaaattta gattggtctt attatgatgg cccacctctt attgcatatt tcattcgatt
      421 ttgcacgact attttttcag acagcaattt aacgctagca ctgattaata aatttatgtt
      481 gctgatgaca tgtatcgtta tttacaccac cgcacgtctt aatctcaata aaagcgcaag
      541 tattatcagc gcgatggtat gcttgttttt cccactgtca atttcttcaa tatgtcgtgg
      601 taccacatac gatgttccgc aacttttttt ttacgcatgt agcttgtatt gcgcaacatt
      661 attcgctaaa gacataaacc gttataaaaa cttatatgct actgctgcag cgattgggtt
      721 acttatgctc tctaaatata gtggggttat tctcgtttta gggttgttgt gctcgattat
      781 tcgccaaaaa cctctacgag gattattttt taatccacat ttttatcttg gggttctact
      841 cagtctgatc atattctcac ctgtcctgta ttggaatttc aagcacgact gggtgtcatt
      901 tttgtaccag cttgatacgc atagcttacg ccatgaagtt caaaatgtaa attttgtaaa
      961 gcttgagaat gtagtggaat atctgtgtac tttaaatttc cttattctta tcatttggca
     1021 gcttagaccc caaaatatag ctcaagctcc tttaggtatc cgtttacaat ctgacgtcac
     1081 cctcgtattt ttttggttta ttatctactt gtgttttatc agcaagattc acatgaattg
     1141 ggttactcct atggtggtta gtctaagttt gctgtgtgga tatgcttatc aaactggtca
     1201 acttaaaaaa ggaatatgct tttatcttag cttctttatt atcttggata ctgcttacgt
     1261 tgcttatcat attttctatc aaaagagcga tataaagcac gaaattgcac aatatttacg
     1321 aggtctccct tatcataaca gttctaacga atttttaatt ggcgatggtt ggactgtagc
     1381 aactgctttg taccctgcca aaggacaccc tttacctaaa acactgtctt gctctaatca
     1441 gccagataac caataccaat attggaataa ttcttattta agagagattc aaagcaatcc
     1501 tgatgcgcag gtgtggtatt tctcaaccag ccatgctttg agctgcttga ataaatattt
     1561 caataattgc cattattttt caaaagttct tttctctaat agacataaaa cattcttacg
     1621 tacctatgta tgtggaaatg aacctagcta atccatcaga acttctaaat aacttcaatg
     1681 ccactcattg atactaaaat tgaagctatt ttttgatatt gagaaagtct ggttcttgca
     1741 aataaaacat tagattcatt ccggtgaatg caatttaaac tattgtttgt tgtcattttg
     1801 ttttctgcta catcttgggt taagacatgc cagaaccacc ggcatgtctt aaccctgtca
     1861 atttcattgc tcaggagaaa tcatcttttc cggtttcaca aacgcagcaa attgctcggc
     1921 ggttaaaatt cccagtttca cggcggcttc ttgcaatgaa atattgtcat gatgagcggt
     1981 tttggcgatt ttagccgctt tatcatagcc aatgtgctgg ttaagagcag taaccagcat
     2041 gagggagtgg tgtaaatagt aatcaattac cggctgatta ggctctattc cctctgcgca
     2101 aaattcctgg aaggagtggc agctgtcggc gagcaggttt aatgagtgca atacgttaaa
     2161 tatgataacc ggtttaaaca cgtttaattc aaaattgccc tggctgtcag caattccaac
     2221 ggctgtatca ttacccagta cctgagcgca aaccatggtc atggcttcgc attgtgtcgg
     2281 gttgactttg cctggcatga tggatgagcc tggctcattt tctggaataa tcagctcacc
     2341 aatcccgcaa cgaggtcctg aggcgagcca gcgtatgtca ttggcgattt tcattaatgc
     2401 gcaggcgaga gttttcatgg caccgtgcgc gtgcaccagg ggttcatgag aagccagagc
     2461 ggcaaatttg ttaggggcag tcacaaaagg cagtttggtg attttagcta tatgttcggc
     2521 gactttgact gcgaattgcg gatgagtgtt caatccggtt ccaactgcag tgcccccggc
     2581 agccagctca tataattcag gcaatacctc ttccaggcgg tgaatgcacg catccagttg
     2641 ggcaacataa cctgaaaact cttgccccaa agtcagtgga accgcatctt gcaagtgggt
     2701 tctgccaatt tttatgatgt ttttgaatgc gtccattttc gctgcgaaaa cctgacgtaa
     2761 attgcgtaca gcaggtagca atttttcgtt aaacgctatc gccgctgcaa tatgcatggc
     2821 tgttgggaag gtgtcattcg atgattgaga catattgacg tgatcattgg gatgaatagg
     2881 cgacttgctt cccagggtgc cgccagccag ctcaatggcc cgattggaaa tgacttcatt
     2941 ggcgttcata ttcgattgtg tgccgcttcc cgtttgccag acatgcaagg gaaaatgctc
     3001 atccaatagc cctttgctca cttcttctgc tgcctgaaca atcaaatccg ctttgtcttt
     3061 tggtaatttg cctaattcaa gattggtcag ggcggctgcc tttttcagga tgccaaaggc
     3121 atgggttact tcgcgtggca taatgtcttt gcctatattg aagtgatgca atgaacgttc
     3181 ggtttgggca ccccaatatt tatcagacgg gacttcaatt tcacccatgc tgtctgtttc
     3241 tacacgtgtt ttagccatgt tatcaattcc ttagcaaagt taatctgttg attacgttta
     3301 tttatgtcta gctgctttat cagcgagttg ggaaagaata aaatcaatcg ccaaaattct
     3361 agcatagcag acgtaaattg gtaatttttg ttatagtcag ggcttacgaa aaacgccaag
     3421 atcaaggcaa aaaatatttt ttacaaagag attggtgttt ttcgtaagtc ctgatagtgt
     3481 catttatcga aatgaggaat cattggttgt gagaacaata cgtatctatc aacccggcga
     3541 ataccagccg gggcaagttc tggaattatc gcctgaggcg gggcagcatg ttggtgtagt
     3601 cttgcgcatg gagcaaggtg aacaactgac tctgtttaat ggggacaatc aagagtttac
     3661 agcattcatt gagagggtca aaaaaaagca ggtattcgtt agaattgcct ctgttcttga
     3721 ggtgaacagg gaatccccat taaagataca tttggctcaa gccatttcca agggcgagcg
     3781 gatggaagtg gtgatgcaaa aatcggcaga gttaggagtg gcatgcatta cacctatcat
     3841 taccgagcgt tgccaggtta aaatagacaa agaaaaaatg gcaaaaaaaa tgcaccagtg
     3901 gcttaacatt atcattggag cttgcgagca atgtgggcgg aatcaaattc cagagttgcg
     3961 gcagccagtt tatttggatc aatttgtcag agaagccaaa gaacatctca agctcatttt
     4021 gcatccggca tttagcaaga catggcgcga ttatcctgtc caacctcctg atgttgcctt
     4081 gattattggc ccggaaggtg gtttcagtga tgaggaaatt acattgacca gtggctatgg
     4141 atttctgcct ttgtcattgg ggccgcgagt attgcgcacg gaaaccgcgg caataactgc
     4201 gctaagtgtt ttgcaagctg ccggaggtga tctataatag acttctttcc aacttggtat
     4261 aagctgcgtt ttttgtttta tagctctatt acaagtattt ctggagtatt atatgtaaac
     4321 tactcttttt gaaaatcttg tgtttgctct aaaacaaaac acacaggata cacaggaaag
     4381 attttgggtc gaatagcata atctggtctt ggaccagacc tttatatttc taataactga
     4441 ggtttattta tgagtgatca tattaaaaca gtaaccgatg caagctttga gcaggatgta
     4501 attaattcat ccaagcctgt tcttgttgat ttttgggctg agtggtgtgg accatgtcgt
     4561 gcattgacac ctattctgga agaagtggca gccactcata gcaaggatat gacgtttgct
     4621 aaaatcaata tcgatgaaaa tcctcaagct ccagctaaat ttggtgtgat gagtatccca
     4681 actttgattc tttttaaaaa tggtcaggtt gaagcagtta aaatgggttt gctgtctaaa
     4741 tcgcaattaa gtgcttttgt tgaaagtaat ttgtaacggt attaaagtta aaaagccctg
     4801 agatgaaaat ttatttacag ggcttttact tttttatggg gcttgtgtta aaattaacct
     4861 acttaaaggg attttttctt aaagatttaa aagttaaaat caaaaaagtc aaaataactt
     4921 tttattttta gtttaaaaat gttggatctt gttgtaaagc tgtgatagcc tgtagctata
     4981 tgtgacatac tttaaaacaa gtgagccaca taggcagcgg attccttagc tgctattccc
     5041 taacataatt ttttcttcgg atattcaatt catttaaaca acaaatcaag tgagaagtat
     5101 gaatcttagt gaacttaagc aattacctat tgccgatctc gttaatatcg ctcaagagat
     5161 gggtgtggaa aatacctccc gtatgcgcaa gcaagacatc attttcgcca tccttaaagc
     5221 tcatgcttta aaaggggaag acatccatgg cgatggtgtt ttggaagttt tgactgatgg
     5281 ttttggtttc ttgcgttccg ctgatggctc ctatctggca ggccctgatg acatttacgt
     5341 ctctcccagt caaatcagac gtttcggtct tcgttcgggg gatactattt ctggtaaaat
     5401 ccgccccccc aaagacagcg aacgctattt tgctttatta aaagttgatc aaattaacta
     5461 cgatacaccg gatagcgcaa agcgtaagat tttatttgaa aacttaactc ccttgtttgc
     5521 taccgagcgt ctggttatgg agcaaggtaa tggcagcaca gaagatctca cggcaagagt
     5581 agttgattta tgcgctcctt tcggccgtgg acagcgtggt ttgatcgtgt cacctccgaa
     5641 agcaggtaag acattaatgt tgcaaaacat tgctcgctct atagaaaaaa attatcctga
     5701 atgttatctc attgttttgc tgattgatga acgtcctgaa gaagtaacgg aaatgcagcg
     5761 ttctgtcaaa ggggaagttg ttgcgagtac ttttgacgag cccgccaatc gccatgttca
     5821 ggttgccgaa atggtaatag aaaaagcaaa acgtctggtt gagcataaac gtgatgtggt
     5881 tattctgctg gattcgatca ctcgcctggc cagagcttac aatacggttg taccggcatc
     5941 aggtaaagta ctgactggtg gtgttgatgc gaatgccctg caaagaccca agagattgta
     6001 tggtgcggca cgtaacatag aagagggcgg cagtttaact attattgcaa cggctctggt
     6061 agacacgggt tccaaaatgg acgaagtgat ttacgaagag ttcaaaggta caggtaacat
     6121 ggaaatccac ttgagtcgta atattgccga gagaagggtt ttccccgcga ttaacattaa
     6181 tcgctctggt actcgccgtg aagatctcct gttgagcccc gaagaattac agcgcacctg
     6241 gatattgcgt aaaatcctgc aatccatgga tgaatgcgat gcgattgaat tcttattgga
     6301 acgcatgaag aatcataaga caaatgccga attctttgat gcaatgaagc gtcaggaata
     6361 atttaaatga ttaattaacc ccgtaattag cggagcataa ttcggggttc aaattcgcct
     6421 tattttcagg tttataattc atgctcatag cccgtattac gctgtgctgg tacgggttta
     6481 cagtaagctt ctttttgaga taattaatga agtattcaga tctgagagat ttcatagccc
     6541 aacttgaatc acgtgaatta ttaaaacgta ttgattatcc tgtatcacct catcttgaga
     6601 tgaccgtagt cagcgataaa gtgctgcgct caggagggcc agcccttctg tttaccaata
     6661 cccccaatta caacatgcct gtactgacca atctttttgg tacggtagag cgcgtggctt
     6721 tgggaatggg tgaggaatca atagtggctt tgagggagat tggaaaatta ttggctgctt
     6781 taaaggaacc cgatcctccc aaaggcttca aagacgcttt tagcaagttg cctttattga
     6841 aacaagcgct gaatatggca cccaaatatg tcagtggagc cgagtgccag actcatgtgt
     6901 gggaaaagga tgaagtggat ttaacgttat tgcccatcca aacgtgttgg cccggagatg
     6961 ttgctcctct aattacctgg ggtttggtta ctactcgtgg cccacaccag tccagagaaa
     7021 acatgggcat ctatcgccag caactattaa gtaaaaacaa attgatcatg cgctggttat
     7081 ctcaccgcgg aggtgctttg gattaccagg cctggcaaca agaatatccc caagaacgtt
     7141 tccctgttgc ggtgacttta ggcgctgatc cagccaccat actggcagca gttactcccg
     7201 ttcctgatac tttgtctgaa tacgcttttg cgggcttgct tagaggacaa cgaacccggt
     7261 tgactcgatg cattggcaat gatttgcatg ttccagccag cgcagaaatt gttttggaag
     7321 gttacctgga gccaggaaat gaggcacccg aagggcctta tggcgatcac accggttatt
     7381 ataatgaagt ccaatctttt cctgttttta cggtagagcg tattactcat cgcgacaaac
     7441 ctatttacca cagcacttat accgggagac cgccagatga gccagccatt ttgggggttg
     7501 ccttaaatga agtgttcatt cccttgttgc aaaaacaatt cccagagatt gtggattttt
     7561 atttgccgcc agaaggatgc tcttatcgtt tggctgtagt cactataaaa aagcaatatc
     7621 caggacatgc taaacgtatt atgatggctg tttggtcttt cttgcgccag tttatgtata
     7681 ccaagttcgt cattgtttgt gatgatgatg tggacgcgcg caattggcaa gatgtcatat
     7741 gggcaatgac gacacgcatg gatccgtccc gcgatacagt catggtagaa aatacaccca
     7801 ttgattatct ggatttcgct tccccagttt caggattggg ttccaagatg ggcatggatg
     7861 ctaccagtaa atggccagga gaaacacaaa gagaatgggg taaaccaatt acaatggatg
     7921 aagatgtgct taatagagta aatagttatt ggtccttatt aggattaaaa taatgacaga
     7981 taaaataatt caagctcagg tggaggacat ttccccttta acggacagca tcgtgcgatt
     8041 ggtcttaatg cctgatgaat acgttgatta tcaagccgga cagtatttgc aaatcctgtt
     8101 tgcaaatgat gcgtttagtt attcaattgc gaatgcgcct ttaggttctc atcgttatga
     8161 attgcatatt cgccatagcc aggataatga ttatcatcaa tcgttattgg ctcatatcaa
     8221 acaatatgga tctctgcgct taaggttacc tttgggtgaa tgcagtatcg atcaattaca
     8281 cccccaaaaa cccattttat tcattgccgg cgggactgga tttgcgccaa ttaaagccat
     8341 gattgagcaa ttactcgcag attcggacag caggccattt gagttatttt ggggagcacg
     8401 ctcacaaagc gatttataca tggatgaaaa agtaaggagt tggggctctc atgccagcca
     8461 cttccaatat ttttcgctat tatccgataa aagtgaggat actttggctt ctcgagttct
     8521 tgccaggcat gcaaacattc tacgggactg gcaaattgtc atcagcggtc ctttcgatat
     8581 ggtgtacagt actcgtgata ccttggttgg ttacggcata tcgcctaccc agatgttttc
     8641 agatgccttt agttttgaat ccaaaacaga tgatgcaaaa aactgagtgg aaggagtgaa
     8701 ttaatggaaa ctttatatca aattttagga ctcattggcg ccggattgat tatttttata
     8761 ttgtaccgat ttattaaggg aagcccagag caatttagca aggaaaatat aagcaaaagc
     8821 tttatgacga tgggggtttt aggtctgatt ttaataggtt ttattgcctt gctggtgttg
     8881 atgttgagga atacataacc agctccatgc cctgttggca tctccacctc ataagctagt
     8941 gagacgggat agctttctga taactgccct tgtttttaat taaataatca tcaatattgg
     9001 ggttgttgat aggctcctga ctcgtatcag gaagtaactg gcattcttcg acataagtta
     9061 tttggctgct gtcatcaact aacaggcggt accatgggtt tcttgttgcg aattcatgct
     9121 gagttgcccg gggattgtat ttccctgaag cctggaatat gggatccaca tcaataacaa
     9181 tcgcccggta tctgatgcgt ttatgaataa ccagatcacc gatattgtat ttggctattt
     9241 tattcataat gagttccgtt tttgagcatt atgacaaata tatcggtaat tccgctttta
     9301 acttgagctt actgaattaa agtgggcgcg ggagatttcc cataaatcct ggctaaaata
     9361 atccccgctt cataaagcaa acacaaaggc aaggctaatg caacttgaga aaaaacatca
     9421 gggggcgtga gtagcatacc aataataaat gccccaacta tcacataagg cctgattttt
     9481 ataagtgtct caaccgtagt gaaatgggtt tttaccagta caagacaaat tagtggaacc
     9541 tgaaaagaaa aaccaaacaa aagcagcatg cgtgtaatga aatctatggc gtaagccata
     9601 tccggcatga gtcgcactcc ttccggaagg gcctgagcaa aaaactggaa catataaggg
     9661 aggacaagat aaaagcaaaa tagtgtccca aagacaaaga gcaaaagact taaaatgatg
     9721 gtgcctctga tttgttcctg ctcgttttta taaagccccg gacgcataaa acgccaaagt
     9781 tgcaataaag caaacggagc agtgagcaac aaagcggtat ccactgcaag ttttaaaggg
     9841 gtgaaaaccg aggaagtaat ctgtgtggca atcaagccct cttgattcgg taaagaacgc
     9901 aataacggcc ttaccaggag atgaaacagt tggtctgata tgaaaaaaaa caatagcgct
     9961 aaagcgccaa accagagaag ggtatagatt gctctttgcc gtaattcaat taaatgaatt
    10021 aacataaagc tcattggagt tgaatgccga gatcatagaa taaaccattc catttgattt
    10081 ttctaatttt gatcattgcc tgacacagta gcatatgaac ccattgctat gggttcaatt
    10141 caagccgggt aaggaattct acttttaaca ccgattcttt caggtcggat ttctttcaga
    10201 cgattgaatt gatcaacaaa aacaacagtg ggttcatgtg ctgcacaatc ctcttccaga
    10261 atttgcgtaa aagaggcgat aataaccaaa tcgccagggg ttaccaaatg agctgccgct
    10321 ccattgacac agatatccgt tgaacccttt tcacctgtaa tggcataagt ttcaaatcgg
    10381 gttccggcag tgatattcca gacattaacc gcttcatagg gcaatatgtt tgccactttt
    10441 aaaagctctg gtgaaatggt gatactccct tcataatcca gatccgcctg tgtgacgcaa
    10501 gcacgatgaa tttttgattt aagcattttt ctataagcca ttatttactt cctaaattta
    10561 agcctggaaa aagcaattgg ctaaatacga ctgagtttat ttgttacatg gctttttcca
    10621 gattgaaata actaaaaaaa atagataaga atatcaacct tggtcattgt ggttgaattt
    10681 taccccatac cttggagttt acgctatata aatttttaaa attgagggag aaggctttga
    10741 gttattgcgt attgtcgggg tttgctggca cttgacacta acctacggta aaattaacag
    10801 gcttccccaa tggggtatac cttgtgatgt caacaagatt actgaattaa ttttatccta
    10861 acataggtga tagaaagtga ggcatagccc acgtaaacgt tttggtcaaa atttcttaca
    10921 agacaaatat attattaatg agattctacg cgctatcaat cctttggcgg atgataatat
    10981 gctggagatt ggccctggtt taggcgcttt aactcagcca ttattgcaaa aactgaatcg
    11041 attaacagcc attgaaatcg atacggattt acaaaactat ttaacccgct tgcctgtatc
    11101 tcaaggcaaa ctgaatttaa ttcctgctga tgcgttaacc gttgactttt gtcaattcgg
    11161 cccccattta agagtagtgg gtaatttacc ttataatatt tctactccat tgctgatcta
    11221 tttattgaaa tttataactt gtattgatga catgcatttt atgttacaaa aagaagttgt
    11281 agaacgaata gcagcaaccc acgggactaa agcctatggg cgactaagcg tgatgttgca
    11341 gtatcattgt gaggtagagt atttatttga tgttcctcca gaagcgtttg agcccaagcc
    11401 caaggtagac tcagcaatag ttcgattaac accttatcgt gtttcgccat ttgagtctgt
    11461 aaacactgag aaactggaaa atattgttgc gaaagcattt gctatgagac gcaaaactct
    11521 tactaataat ttgaaaggga ttatctcatt atctcaactc aatgatttag gtattgatgg
    11581 aggtaagcgg ccagaacaaa tttcggtggc ggaatatgtc caactagcga aatttatttc
    11641 caattagtgt aaaattatat gataagttat tgtggaggtt gtcttgtttc atcgtcagcg
    11701 taaaagctct ttatctgctc agtccaagtc attaaaagat ttaatccgca tggtgaccgc
    11761 agagcagttt ctgagtgaag agaaacgtca ggtattactc cagaaaatga gagcatttag
    11821 cgggttggaa gcatctcgct atgagagttt atgttctgtt cttgtcgaga atctggtgaa
    11881 ctattgtcaa aatttgccgg aaaccgcgaa cagttattat tcccagcccg gtggtttggt
    11941 cgatcatgct ttaaacagaa cagaggctgc attgagtcta ttcaaggagt ttaccgttca
    12001 agaacagcca gatttgcttt ccgaggaaca gaagctttgg caatatgctc tttattcagc
    12061 ggcaatttta caaggccttg gcaaattatt tgatgagttt cgcattaatt tgtttgatag
    12121 caatggtcaa ttgttaaaac aatggaaccc tctattagaa agcttaatcc aaactggaag
    12181 ttattatgat tatcagtttg agaaagaagc ggacgcggag ttacgccgcc ggctgaactt
    12241 gttgctggcc agaactttga tgccggccag tggttttgcc tggattgcct ccaatccgga
    12301 agtattggct gtttggttag ctttactgaa tgaagattat cgttctgctg gtactttggg
    12361 cgctatttta attcgagctg atgccattgc gatacagcgc tattttctgg cgtttatggc
    12421 acgaagtggg gcaagacgaa gtggccgtta tggcagagcg gtaactttta ccggcggtgt
    12481 acctgaatcc ataacagaaa aggaacaggc aataggggtc gaattcatcc aatggcttat
    12541 caagtcgttg gatgaaggtc gaattatggt gaacaaggcc ccattgttta tggttcctgg
    12601 cggcatgttg atgtgccctg aaatgtttca attgtttgtt cgtgagcatc ctgaatataa
    12661 aaattggcaa gccatacaga atgggttttt gtcattaggg cttcatggcc gggatgcggg
    12721 gggcaatgta accagtcgtt ttgaacaggc tcatactcag caaatgtata ccggagtcgt
    12781 ttttgcagga tatggggttg ctttacccaa ctctgtccag gtgcatcatt tgaatactgg
    12841 caaaatagaa tccatgtcag cactggagtt aattcataaa tcacaataca ccagccaatt
    12901 cacacagcaa tcaagtgcga ttgcaatagc gcctttgcaa cgattggcag caaatggcca
    12961 atggcagaat attgagcagg atatgtccca attgacattt ggagcaaaac gcggtgcctg
    13021 attatgcaat aggggatgta caaggttgtt atgatccttt acaacgtttg cttgagctaa
    13081 tcgattttaa cgagaaggaa gattgcttgt ggtttgttgg ggacttggta aaccgcggtc
    13141 ctgattcatt ggctgtatta cggtttattt attccttacc tgtgaaacct aaaataacct
    13201 tgggtaatca tgatttgcat ttattaggct tattattcgg cgggcaaccc tggaaagggc
    13261 atgatgatac tcttgaagag gtgatgctgg ctgatgatgg agaggagctt gggcattggt
    13321 tgcgcaagca atcccttctt tgcaggtcat ctgagttaaa catagtcatg tgtcatgcag
    13381 gtattgctcc tttatgggat ttatcgaagg ccgtgggctt ggctaatgaa ttggaagcgg
    13441 tgctttcagg cgattcatat cacgaatttt tcgcccagat gtacggcaat aagccggata
    13501 tttggtcaga tgatttggtt ggactggata ggttacgagt cattaccaat tattttaccc
    13561 ggatgcgata ttgtgatgcc catggacgat tagatttggg ttataagggg actttatcca
    13621 aagcccccaa tcatctgtat ccctggtttg aagtgccatg ccgaaaagaa attgaaatgg
    13681 atatcgtttt tggtcattgg gcggcgctca tggggcgaag ttctcatccg cgaattcatg
    13741 cgatagatac cggatgtctt tggggagggc aattaacggc attacgcctg caggacaggc
    13801 aacgcttttc ggtacccggg tatggggtta gtcgtttcga ataaccttga gggataggca
    13861 tttttataaa cacaggcgat aacaacggtg aaatttttaa ataaaagcac agcattgtta
    13921 ccggtaatta ttttatcggg cctgattagt tttcatgccg caagtgccaa gacttcttca
    13981 aatgccgata attcacttta ttcctggaca gaaaatcctt cagctgaagc acagagagtg
    14041 gagcaagcgg aggaaagact aaaagcagct attgccaacc cggaatacat gctgaaaaat
    14101 tggatagagc tgcctacctc gcctgagtca aaaaagaaaa caaggcacca gttgacgata
    14161 agagaagcca tactgttggc cttgcgttat aatcctaata tccagaatgc ggaattagac
    14221 cgcattattc agcgctatca attgcgtttg gctaataatg agtttgaatt gcaatatgct
    14281 cttggtgctt caggcgttgt gcaaaaaagt acttttgagg ggataggcag ttctacgaac
    14341 cattcttttc tggccagtcc tgaattggat ttgaaaacta aaataggcac caaggcatcc
    14401 gtatccatcg ataacaatgt ttcgaccaac aacagctata atccggtatt gaatttatcc
    14461 attactcaac ctttgctccg tggatttggc aagtcagcga atgaagcaaa actgttggat
    14521 gcgattgata aggaatggtt aaataaatta agtttacagc aatcggttat ggatcagatt
    14581 acgcaggtga ttacggcgta cagggctttg attttaagtg ggaacaattt agagaatcag
    14641 cgcttgcaat tggaagaagc cagaaaatcc tatcgtatta atgaaaaaaa gattgctgcg
    14701 ggacaattag aacctaccgg aaatattcag caatcctacc agatagaatc tttgagtttg
    14761 atggtagaac aaggtgaaaa tgattttaaa ttagcctctc aggaattatt gcaaatgatt
    14821 gggcttgatc cggatttgca tatttcagtt cccagtgatg tgacgataaa ggaaattata
    14881 gtgcctgact tgcaagagtc cattgccatc gcattaagtc acaacactca gtatctggcc
    14941 cagaaaatgg ccttgcgtgc ggatgagagg gcttatactg ttgcaaaaaa tcagcaactt
    15001 tggcagcttg atttggcggg tactgtgcaa ggcggcgcag tcaatgatgt caccgggacc
    15061 agcagtggtc taagcagcat ttacaatggg aataatatca cggaatcagc ccgggtcacc
    15121 ctgaccattc cattaaatga cattggccgg cgtagtcaat taattaatgc taaagtccag
    15181 ttggaaaaag acaaaatcaa tcttatcgca gccaaaagag ctttaattac cagtgtaacc
    15241 aataccatca ataatattca aagcttggcc aagcgctatg agctggcaca aaaacaagtc
    15301 aaattggcag agcagtctta cgctttggaa aagaaaaaac agcaggcagg aatatcgagc
    15361 gccctggatg tgaacaatac tcagaatcaa ttgattcagg cacagtcagg attgataggg
    15421 gcaaaaattg catatctcaa tcagttatcc gcgttgcaaa ggattttagg gaccacgctg
    15481 gattactggc agattaaact aaggtatggc ggatgaacaa ttatctgaaa atattctcta
    15541 tagtgttatt gattttaatg ctggtttctt gtggcagttc tgataagccg caaacctctt
    15601 tattaaaaac gtatgaagtg aaacctcaac ctttacacaa gaccttgcat tttaccggga
    15661 ccgtgcaacc cataagggaa agcagcctga ccagtccaat ggaagccgtt gttgaaacca
    15721 tgcatttcca ttatggccag atggttaaaa agggagagat tgttttaact ttaaattcca
    15781 atgaattgca aaaacagtat aacgacacat taacggatta cttaaaggcg aaagacagtt
    15841 attccattgc caaggcaaaa tttgtaggaa ccgaggagct gtggaatgcc gggctaatct
    15901 ccaaaaacaa ttacttaagt gaaaaatccg ggttggatac agcgcgagtc actttaatgc
    15961 aggccactcg caagctttct gaaatgttgg aaaaaatgga tgataaaaac actcagaata
    16021 tatccaatct cagtctggct gattttgaca aagtgaggaa gattcttacc tcaaatcata
    16081 atttgattca tttaaaggct cccagtgatg ggatcatgct gtaccctccc aaatcaggag
    16141 aggataaaaa caatcgagtg accgtaggat cgaccataaa gtcagggcaa gtcattgcct
    16201 tgataggcga tttgaaagga atcagtgtgg aaattgatgt cccagagata gacattgata
    16261 aaattcgccc gggaatgcag gcaacaatta gtggcgttgc atttggcagg catcagctga
    16321 aaggtaaatt ggtggcggtg aatgcgcagg catcgaatac cagcacggga ggattaccct
    16381 cctttaccgc tgtagtggag gtcaattccc taactcctga gcaacagtca tggattaaag
    16441 tggggatgag tgcctccata gagctgaatg tggaatcgaa taatcaattg ttgatcccta
    16501 tctcggcggt taaaagggaa aaaggcagca gcattgttca ggtccaatta gccaagggga
    16561 aagtcgagaa acgaatgatt actaccggtg ccgctcaggc ggattcagta gtggttgaaa
    16621 gtggccttaa atcaggagat gtcgtggttt atgattgaca gacctttaat ccaactttct
    16681 gatttggtga agacttatca tcttgagggt atcagtacca cggtattaaa agcagtgtct
    16741 ttgaccgtaa acgaaggtga tttgctcgct attgtcggtg cgtccggctc tggtaaatcc
    16801 acgttaatga acatcatcgg cttattagat aaaccggatg caggaactta taccttaaat
    16861 aatagaaatg tggccagctt aagcgacgat gaggcggctg atttgcgcaa tcaaaatata
    16921 ggctttgttt ttcagcagtt taatttgctt cctcgtttta cagcaatgca aaatgtggct
    16981 ttgcctttga cgtaccgagg ggtcaatccc actttgataa aagaaaaagt ggagcaagca
    17041 ctggataaag tgggaatgcg ccaatacatc cgccacaggc caacccaatt atctggcggt
    17101 cagcagcagc gagttgctat cgccagagcg ttagtgaccg acccacaggt tattctcgct
    17161 gatgagccga ccggtgcgct ggattcaaga acaggtagcg aggtaatgaa tttgtttttg
    17221 gccttacacc aggaaggccg gactattatc atggtgactc atgatgagca cgtggccgcg
    17281 caatgcaaac gccgaataac cttagccgat ggggcaatta tagcggagtg cgggcaatga
    17341 attttcttaa tcattgtcaa caagccttgg ttaatttaac agcctccaag ctccgatcgc
    17401 ttttggctgt gctgggaata ctggtaggga ctgccgctgt cgttgccttg attagctgcg
    17461 gacagctggc taccgagaag gcgttggagc aattcaaggc attgggtacc gatttgctgg
    17521 ctgtttctgt ttatcaaaag gtgcccagca aatcccatag tgaatccgac tcgttaacaa
    17581 tagatcaatg gcagcagata gccgagcgta tcccttatgt gctaagaatt gcgccttaca
    17641 gcacggctta ccaaacttta agctttaatg gcaaagtgat gcagggggcg gtgattggcg
    17701 ccgatgaaag ccttgctgat attatccatg tgaccttggc gcaggggcat tttgtttcct
    17761 ttttggattc ttttgagcat ttttgtgtta ttggccatag cctggcacaa caaatcaaag
    17821 aaactaccct ggatgatccc attggcaagc aattacgcat aggccaatcc ttgtatacca
    17881 ttatcggcgt tgccgagcgt tggaaagaaa atggcttttt taatgaggac attaatcagg
    17941 ctgtcattat tcccctggct ggaatgagcc tggtcagcaa ggacgccaaa atcaataatg
    18001 cggtattgct tctcaagcct gacagcccca ttgatgaggt cattaatgaa ataaaggaaa
    18061 tcattaattc tcaggcaccg aagctcagtg tttttccgcg cagcgccaaa caaattattg
    18121 ccagcatgga aaatcagggt cgtattttta ccttgctgtt agcggtcatt ggtggaatat
    18181 ccttgctggt tggtggcatc ggtgtcatga atgtgatgct ggtttcggtc agcgagcgca
    18241 aaaaagaaat tgggattcgc aaagcagtgg gtgcgaaaaa tagagaaatc caggccttgt
    18301 ttttggtgga gtctgtcatg ctgtctttgc tagggggggt gctcggagtt attttaggat
    18361 tgatttttac cagaatagtc gcttatttca gtgactggac ttttaccgtc tacttacttc
    18421 ccccaattgc tggttttctc gtttctgctg ccactggaat atttttcgga ttttatccgg
    18481 ctcgacgggc ttccaagctg gaacccatgg tttctttacg gagtgaatag ctttgtacaa
    18541 tcttgccatc aagacatggg ttgccatgga tttacgctat tgaccacatc atggatttcc
    18601 gtgggcgctt aatttaagta caatactgga cactggacta ggcacttgga cttaaaatca
    18661 gccaaagtgc cacaaaaaca gcagggtatt tttttgccct atgcattaaa tgggctttta
    18721 tagaggctaa tttttaataa atgagctatc ttttaagaat accagtatga tttaaacgga
    18781 ttgtttttta taacaattaa agagaaataa aagctgggcg aaatacatat tatcgaacaa
    18841 taaatagaac tgaggggaag agctatgaaa aagtgggggc tcgctattgt tgcactgatg
    18901 ttatttaacc aaataaccta tgcaagtcaa agctggaatc aatgggttgc cggggtgcgt
    18961 gaggaagcct tacaacaagg gattccaccc agtttatttg atgaagcctt tgccggcatt
    19021 catgaaccaa gccggaaaat aaaaggtttt ttacgctctc aacctgaaca caggttaacg
    19081 ttcatgaaat atcgtaatac cagagcagac aattaccgaa ttgtgattgg aagaaaagag
    19141 tacaagagaa acaaggaaat actggaagag attgcccaac aatacggagt taatccttgc
    19201 tttattgtgt cgttttgggg tatggaaacc agttacggct cctacatggg aaatttcccg
    19261 gtaatcaacg cgctggctac tttggcctat gattcaaacc gtaaagactt tttccgcaag
    19321 gaattgttcc tcgctttaca catcctgaaa ggcgggcatg tcagcctggc tgatttcaaa
    19381 ggagaatggg ctggcgcctc tggtcaacct caatttttac catccagctg ggtcaaatac
    19441 gcggtcgact acgatgggga tggacgaaaa gacatatgga agagcaaacc cgacgttttt
    19501 gcctccatag cgaattacat gaaacaaaac ggctggcagg ccggagagcc ctgggctatt
    19561 caagtgaaac tccctgccaa gtttgatatg aagctggaag ggaaaggcat agtcaaacca
    19621 gtcagcgagt ggaatgcatt aggtgtgcgc acggaaagcg gcgaacccct gccctatcaa
    19681 aatttgcagg ccagtatcgt acagccttac ggtggcccga ccttcctggc gtttcccaac
    19741 tacaagatga tcctgcgtta taataactcc atttattatg ccggggctat cgggtatatg
    19801 gctgataaaa tttgccaggg tcaatgagag atattctgaa gggtggtaaa tgaattcaag
    19861 aggttctttt gatgaaatta tcaaaagaac cttttttatt taagttcgtt tttaatagct
    19921 ataaaagata ttaaaatcaa ataatgcgaa aactgtaagc cgataaattc acttcatatg
    19981 aacagaatca gctcatctaa ttatattttg tgaatagaat accttttgaa caagtctact
    20041 ttctaagcgc ctcatttgtt tatatttttt gcatcaccca atgtgcccaa aaagtttaca
    20101 aacaatgtgc ttaatgtata atacttacga ttttaaatta ggggtgaaaa ggtgaaacca
    20161 gtatttgcta aagaaaatgg ccgttggagc acagtataca acagcgaaaa atatcaagat
    20221 gtgtattttg taggttttag agtaaaattt gatggatcag aaaaattaca aaccctaact
    20281 atcaaaacag ataaaaatta ctcagtggct cttagttatt ctattgatgg tagctctgat
    20341 gtagtatgtg atgaattttc gtggaccaat gccttactgc aggcagcaaa tcaagatggc
    20401 tattgtgcgg ctcgtcccaa agaatttgaa ttacatacaa aagaaagtga atattattat
    20461 gccctaacag aagcaagcaa atcagcaatt tggaaagatc ataaagaagc aatggatctt
    20521 tttagacata gctttactgc tggaactgca tttgccttac ctaataccaa acctgggcca
    20581 aaatttcaga ataacgcatc tgaattgcaa tctacatcat caatttcgat tgatcaggaa
    20641 ttgtcaccac tgagtgaaga gattacaaaa caagtcactt ttaaagaggt atcaaataat
    20701 agaactcgta aagcaaatgc ccctcatgaa aactctcccc aatactcatt aataataaac
    20761 cccgaagtta aaccgtctca aataaaggaa acacatccct cgctaatgag taacattagt
    20821 atgatggtta taggtggttt tatagctgcc gctgggatta ctgcaatcgc tatcgcgttt
    20881 actgtgctta atgcagcaac ctttggtgtt gcaggattat tagtggctgg tctaggtgtt
    20941 gctgcaaccc tttcaggggc aggtctattt gctactggtg cttataaaca tttgcacaca
    21001 attggagagg attcattggg tctctccggt aatctagtgc atcaataaat tttagaaaat
    21061 ttttcttctt cgataagttc tttttgaacg ggctctaaga aatgaagatt gcaaggaaat
    21121 agatgttatt gagtcaaccg ataaaatcaa ataatgtgag aattataaac agataaaatc
    21181 acttaatgtg gacaggttgg tggataaaat taaataatgt ggtcagtaag ttatttataa
    21241 aatcatttaa tgtgagcaga aaattagcga taaattccag aattgtggtt tataagtgcg
    21301 aacaacaaca gctagtcatt ggtatatttg tggtatattc atagcaacac aagttttaaa
    21361 agtgaagacc atacaatgaa gattgaaata atccccgcaa caataagtga ttatccaatt
    21421 gtacaaaatc tagcaagtta ctatgtttat gatcgaactg gatatatggg gtggcaatgt
    21481 tcagaagacg ggatttttga atgtattgat tttaaacatt attttgaaac tccaaatgaa
    21541 aaagcgtttc ttgttaaagt caatgatgag ttagctggat ttgtattgct ggataaggag
    21601 catctcctcg aagcagtcga ttggaatatg ggtgagtttt ttattcttag aaaatttcaa
    21661 ggtaagggta ttgcccctta tgttgctcat aagatattaa aagaaaatcc aggaaaatgg
    21721 tctattgctg tcatgcctga aaatataaaa gcggttaatt tttggcgtaa aattatttct
    21781 gctcttgtta tggaaagtta taccgaagtt tttaaaacaa aagatgaact aaaaacacca
    21841 gagaatcctg atgcgtatgc aatgaatatt tttacgttta atactgaaaa atgcacaaat
    21901 taggaagttg ggataacttt aaccctatta ctaacgcaat gtctctaaac cgactactat
    21961 aggcatccct acgattttct tttaaggcat tatcgaggca atccttgtgg gtaccatttt
    22021 gataagttgc gcctagtcca tctattgcaa tatttaggct gttacaatga atcagaacct
    22081 tcaaatgata tgccataatt tttttataaa tatattgtag tagacaaaag aaaagaccag
    22141 aatgcttaaa atgctttcta tctggtttaa atatcccgcc tagagttgcg ggatgataac
    22201 tcatcacacg cctactcttc atcggctggt tttctacaga ctcgcaaaac tctgttatat
    22261 tgacatggtt aggaaatgcc aaggataaaa aaatgacgaa gatattaatt tgtttaggac
    22321 tgtttttaag cagcaattta cttatggcga aatcattgtc gcaattagat tctgctaatt
    22381 tgctcccctg ttttaacatg gaacaagcag aacgcattgg aaaacaaatc aataagctct
    22441 tacagcatga gttttgcgag gaaaacatca atccaaagaa atttgcctct atcagtcaca
    22501 atatcctgcc caaaattatg acagaaacat ttttaggagt aaccccgcca gaaaactggc
    22561 agcaattaag cgacgatatt ataaaaaact gcatcgcaaa caagaatcta tgcaaaaaag
    22621 cagctcgcaa agagctggaa gaatgcatca aaccgagaat tcctttgatc ctgatacaat
    22681 ttggcccctg gcttgctcaa aattgtccac aattgaataa gtctttgatt gaacaatggc
    22741 caaataaaca ggctactctc aaaaagataa ttaatgaaaa caaaagtgcc gagtaatcga
    22801 gacaggctta attacgagag ttatgcctga taaaaccaca tttatttacc taatttcatc
    22861 aaatataact caccgatatg atctacaacc aagttcttaa aactatgggt ttgtgaacgc
    22921 cattacactc tgctcggtag agagtctttt cagcttgtaa tcagttcgaa aactctagcc
    22981 agaaatcaaa attcgcaaac atgaatttaa gatgacaaat agaacattca ttttaaaaat
    23041 ttattaagtt ttacgatctc caaattgttt taaaagcaaa aattcgggtt tttgctgttt
    23101 gcctaagtct ttggattgct ttctttccaa agctgattca gcctgtcttt atttttttgt
    23161 aaattttggt tgagattccg taatttaata ggtatagcca gagaggtaaa caaaaccccc
    23221 tttattttat ctatgattgt gtaaaatttg atatgtacgg caatttgccg tataataaat
    23281 ataaatgtga tacttcaagg tattgttatg caaactacac accatgactc taaaagtgat
    23341 cgaatagaag ctcgtgttag ctctgaggat aaggatcttt ttaagagagc agctgaactc
    23401 tccggtatga ataattttac tgctttcgta attaaagcat tgaaggatgc ttctgttaaa
    23461 gtgatcaaag aacacgcatt aatagaacta tctttaagag atcagcaatt gtttgttgat
    23521 agtttattaa aggatactgc tccgaaccaa cggttactca aagcggcaaa aagacataaa
    23581 aaattgatat aagaataaaa atcttgaata aatattgtat tgaacaatta aataaatcac
    23641 atgttaaaga taattttaat agtggtgaag attcattaaa taaatatctt cagaaacaag
    23701 caactcaaga tataaagcgt aatttaagcg cggtctatgt attgctcgaa ggacaagatt
    23761 ctactatatt aggttattat accttatgct cttcgagcgt attaatagat agtattcctc
    23821 acgaagtatc aaaaaaatta cctcgttatc cattaattcc tgtaacatta ttagggcgtt
    23881 tggctattga tataaagtta caagggcaag gcttaggaga gttattatta actgatgctt
    23941 tattgagggc aggcagatta agtaatgaac tcgggtcaat ggcggtaatt gttgattctt
    24001 taagtagcag agcctcggat ttctatgaaa aatttggttt tcaaaaattc atagataaga
    24061 ctaataaatt gtttttacct atgagcatca ttaaacaact attataggag agctaaagtt
    24121 tcccagcgaa gtatttggct caagctatta atcataaaag taggcttaag tacacaactg
    24181 acttcgaaat aaacaattac ttgaaaagca aatttattta tcttcatgaa cagcaacgtt
    24241 cagaaaaccc ccgattttct tcctggtgcc ctagtcaaaa agggaagtgt ccaaaaaaac
    24301 ccaagtttta tggctattaa atcgatatat agataatctg ttttctcgaa gcaacgattt
    24361 aataatcata actaaataat caatatggtt gcttccatat tcacaaatga tcagcaagcg
    24421 taggtcaggc caaggtccaa cctttctgga gtttcccaac tacaagatga tactgcgtta
    24481 taataactcc atttattata ctggggctat cgagtatatg gccgataaaa tttgccaggg
    24541 tcaataaata gtattctgat ggttaggtaa taaatgatga agagttcttt tggtgaaatg
    24601 aataaaagac ccctttttta ttgagcgact cttaaaaagc catttgcttt attctgtgct
    24661 tttggcaagt gacatgatcg cattcaggtt ttgtttacat gctaggtgtg gttttttccc
    24721 gaggcggttg tgagtttgat accataggtt ttattgaata ggcgccgctt gggaaaaaac
    24781 aattgtcagt aaggattgcc cctgtaatca gatgggtaag ccgattggct tcggcacatc
    24841 tcgaatcagt gacaacctct ttcgcttttt catctttatt tcgcataaca atcctgtgaa
    24901 gttaatcttt gcagaggaca ccatgatggt ttcatgtcat acaaacgaag caaccggata
    24961 ccgagttgag tccaaagctc gatcttgatt ccttcgctaa cgcttgcaac aaggagtatt
    25021 tcatttgtag tcccttgcgt tggtgcaaag caagttaagt taaaaataat cgaccagtta
    25081 atgtctggtt aaggatagca ttatatcatt accgatcaat tgcgcgcgtc gtatgattta
    25141 agagtaataa gtaatgaaag acaaaacaat acccggccct gatgcaaaat catttaataa
    25201 ggaagagttc tctgaaaacg tagcaaaagg agccattgtt ggcgcaacca gcacgaccat
    25261 gacgtttggt ttattcgaca agcctttggc cattgctact aaagcttctt cggaagttgc
    25321 tcagcatcca tggcgcggta tcaaaaattt gtttgctggg gcgatcaacg gaaatttttc
    25381 gaccagctgg aataatagcc tgtatgtaca ggcttatcga gcttatttag agcaccctgt
    25441 caaaggttat cctgttgctc ttttaaactc ctgcctaaag aatgtggtgt tttttcccgc
    25501 aaaatatggg agtgaaaggg cgctttatgc gctttcaggg aatgaacagc aagcaaaaaa
    25561 ttattccgga ttcctggccg ggctcatgac cgtttatctc actgcacctg tttctgttgt
    25621 gaaagcaaga ctgatgacta acgtacctct taatacactc agtttaaaac gattgggctc
    25681 aggcatgaat gccatcgcga tgcgtgactg cattcaatat ggaatttatt ttaatgccaa
    25741 agagtggtta aatgaaaaaa ttagtgacgg tgttatggcg ggtggcatag ctggcattgt
    25801 ggggtatacc tttagtaatc ccttcagtgt aattggactt aatcaaaaaa cggctgttga
    25861 atcagtaaac attcccaaca tggcgaaacg aatttatcag gcgaatggtg tgaaaggatt
    25921 ttatccttta gtcggcctgg ccgctttggg catgtttgct cgtggcgtgg ctatccatca
    25981 gggcactaaa ctattcgaat ccttacagga aaacgaagct tatggaagtc caacaaggaa
    26041 gaggtagtaa attcataacg ccataacgtg gtgacggttt aataacggtt taataatagt
    26101 tgccgtattg actcaattac gagcaggcgt agcaactgtc ttaaattttt aattcaagac
    26161 agttgctacg cctgctgaat tagaaagaga ttttatctcg gaacgaacca aagaaggatt
    26221 gcgtgcccgt tctgcaaagg gtattaaact tggcaaaccc aaagggggca tccaggcagc
    26281 gatgtatgat aaagataagg aaaaaattat ttcaactcac ttagcttatg gcaaatattt
    26341 atccctcaaa gcgtttctca ataagccatt gttaccatga accagggtaa gctttaattt
    26401 tcttaaacaa aatggagtat ttagtcctcc cttagatgga agctctttca ctgctttgct
    26461 gagcaaatat ttgacgctcc tcactgatta attcaatcca ttttttaggt gtcatgtcgt
    26521 tttgtatcaa taagttgatc gctgcactag gatattgact ttgtgtaatg acaaaataaa
    26581 agtaagcggt caatttgctt ctgcataaag cccaaccact ttttgcgaca cattgttgca
    26641 aactagcaaa ttcaatttga tcttctttga ctaattgttg cagcttttga atcgtgtcag
    26701 ggatacatgc atcaaaaaac aatgtggtcc cttgctgtcc ccatacgtca cttaatataa
    26761 ttctttttaa aatgttcatg tcacaaattt gttgctcatg gggaataggt ttctcaattc
    26821 tatgtgttac cggttgcccc aacatggaat gtgtccattg ctgataataa gcccgaccgt
    26881 aaattaatat accacccaaa ataaaatcaa aggcagttgg ggctgcatac caaagagcag
    26941 tgaagaattt ggatttgatg acgtccactt tggggtaggg atactgatgg ttattggttt
    27001 taatgatatg tttgcgatcg tattgcattt ttaaaggttc atatttaacc aaggatttag
    27061 cctggtattg attctggcca atacttatcg tttcttcctt ctttggtatt cctccatcga
    27121 ctattgctaa atgaaagtta tgatttaaaa catgttcata acccagaaaa tgacaatcag
    27181 gatttttaga taaggtattt gctaaatgtt gtcgcaatgc ctccccgact ttaatattag
    27241 gatcataaaa atctggatgt ttggctaaat tcattaccca aaatacattg actttattct
    27301 tttcaccagc aggaatatct cgtaacatca ttccccaaca atacccaata acttcgtcgt
    27361 ttttatcctt ggcaataaaa caaatagtct ctttataatg taacattgaa gataggacga
    27421 tatcccctat aaaaggcaaa tcgccaaaat ttgctccatg tgctaaagaa tcgatacgtt
    27481 taatttcacc cattgcttta ttaagttcag cagtattatt gggatctaaa atctgaactt
    27541 ctttgaagcc atagggcttg ccttccatca tttttaacga aaaattaaag gtacattcgc
    27601 catctaactt tttgtggtgt ttaattttaa gaggattttg atttctgcaa tttaacagat
    27661 ctgtccataa ttgatgaatg taaagtaatc tttcatcatt tggtgaggta tttcgcaaat
    27721 tgattaattg ctgggtgaaa cgatgcacat attctgtatg gagataaata gcatggctac
    27781 tgaaatcaac aacaaactct gccgtcatta attttttgat ttcgggtaag tgatattcaa
    27841 caggatctgt atatgatttt actattgctt ctagtgaact ccatttgcgt tcaagtacat
    27901 cgatttttga atacgacatc tatatttcct tgtaataaat tttcggagaa atcttaacat
    27961 ggcaatttac ttggccaaat cctatttggt caataaagag taggctcttt ttaaatttaa
    28021 aaaggaacta tctttatatg gaaaaactat aattctcgag acgggagttt tcattgtgct
    28081 gtgttgacat tagatagaga tcatcagcga tggggtgagg gccttggatc ggattcctat
    28141 tgacacaagc tttctaaagg aatagttgag aatttagagt acagatatct ttcattgcca
    28201 gtttgggctg gatatgcggg aaaacaaagc cttacaatac aataagcttg gtcgaggggg
    28261 aattgaacgc cacaatccat gcggcttcca tcttgcctga tgctccaatt ccgcctcctc
    28321 gtttttttat cataacaaat tccagaatgg agtcacgggt ataaattctg aatggcgtgt
    28381 aagatatttc tcattctctg ctacttacat gatcataaat agccaggttg tattatttat
    28441 tgctttatga tatttccagg ctaaagaaat ctatctattt caatctcttt attatgattt
    28501 tcactttgca aaaagaacaa gcatccttct aagtgccgga atttagggta taaccagagt
    28561 gtattcacag agttatccac agaaaatggg gataagtgat taatgcaatc caggtatcaa
    28621 tacctaataa gtccttgtag ggcaaggggt tttgtaaaat gtgcaggatc tcaggcggtc
    28681 agagattgaa aaataaaaat agttattcac agggggggag atgcaattta gatgttaaac
    28741 gatttacatc gtaacaaaaa aaaaaataaa aaaacagtct tgacgaagtc gatttttttc
    28801 caaaaaaatt tgggcatgct gcgattacgt ataaaccgat taagccagtg tggtaagtgc
    28861 atggacaact atttgattca agccgattag aatgatataa agtgcacctt taatggttgc
    28921 atcgcctaat gtgagcgcca ggagattaaa taatataccg cctttaaaag gcccgacttt
    28981 taatttgaca agcttatgtt gtattcacta attaaaaaat accataccgc acaccgccta
    29041 tccgactatt cttagcgaca taccgctttt gtatttctat ctaattactt caacgcgctg
    29101 tctctttagc taacgcaaag agcaatatcg gtttttttaa tatgtaaagg atgtcaatat
    29161 gcatgtgtta gatcccttaa ctcttgaaga aattcaggaa agttgtgata ttttaaaacg
    29221 agaaaaagaa ctgtctgaca gttatcggtt tgcctgggta atgacttacg aaccgcccca
    29281 aaaagaagtt ttgcattcaa gtgatactga tttcgaccgt tgcgcttttc tctcggtttt
    29341 taataaaaaa acgaatgaaa cctttgaagc agtcgtaaac ctcaacagta aaaaagttgt
    29401 ggaatggaaa cagattgtcc tcgaagtgcc accctatggt aagccgccta ttttaattga
    29461 ggattttcaa aaatgtgagc aaatcgttaa agccgatccg acctggagag ccgcgatgga
    29521 aaagcgtggg cttgatgatg agcaaattga caacattcaa gtagacgcat tttctgctgg
    29581 ctattttgat gaagaggagg aaagagaaca acggattgtc aaagggataa gtttttaccg
    29641 tgataattta aaggataatg gttatgcccg tcccattgaa ggggttgtcg ctatagtaga
    29701 ccttggcaat gagaaggtat tgcgtgtcat tgatgacggc cgcaacgtcc caattcctaa
    29761 agaaaaaatc aattacgatt cggattctta tcctgaaaaa cgcaaagact taaaaccgct
    29821 agatattatt caagaagaag gaccaagttt taaagtaaac ggctgggaag tcaattggca
    29881 aaattggtca ttccatgttg ggtttacgcc gcgtgaggga ttggttttgc accagatcaa
    29941 atacctggat gaagggaaag aaaggccaat tatttaccgg gccagtgtga cggatatgat
    30001 agtcccctat gcggaccctt ccgtgagtca ttactggaaa agcgcttttg atgcgggtga
    30061 atatggcctt ggcaaattgg caaatgaact aaagctcgga tgtgattgtt taggcaatat
    30121 ttattatttt gacgttcctt cggcggatga ttttggcaat gccagattga ttaaaaatgc
    30181 aatttgcatg cacgaagaag acgcgggcac cctgtggaag cattatgaaa cacgcaacag
    30241 caccttcgag gttcgccgtg ctcgagagtt ggtcgtcagc ttttttacta cgataggtaa
    30301 ctacgactat ggcttttact ggcgatttgg acaggatggc agcctcaaac tggaaataaa
    30361 actgactggt attgtacaaa cagcggcaat atttcctgag gctcagtatg aatggggagg
    30421 gaaattgaca ccggagctgg cagcacctac gcatcagcat tttttcaatg cccgccttca
    30481 tatgatggtt gatggtgaaa agaactcatt cagtgaacac gagttttatc ctatcgctat
    30541 gggtggaaaa aatccctatg gaaatgcgtt tggcagcagc cagaaaacat ttgaacatga
    30601 aggtgaggca gctcgctcgg cttgtgcgca aacccagaga acctggaaga tattcaatcc
    30661 caatagcgtc aatggcattg gcacagcacc agcttacaaa ctggaattgc cagaaacccc
    30721 cttactgctg gcagatgaag agagttatat ttataaacgc gggggattcg ccagcaagca
    30781 ggtgtgggta acacaatatg acccacagga aaaatacgca gcaggagatt atcccaatca
    30841 gagttctggt ggttgtggtt taccttctta cattaaacag aaccgtaaaa tagacaatga
    30901 caatttggtc ttgtgggtaa ctctggggcc tacgcatttt ccaagacctg aagactttcc
    30961 ggtcatgcct acttcgatca tttctgccaa gcttcagcct tttggttttt ttaagaggaa
    31021 tccggctatg gatcttcctc aaggcaaagt acaggaaaaa ggggacacct gctgttcttc
    31081 attaacttat taatcgattg ggaccaagca ccttcttgag gagagggtgc tttcctgtct
    31141 ttagttttcc tcttttttat ccagcttgtg accatccaga tgacgttcac tgcgttcatt
    31201 gtcttgtttt tcttgttgct tttctttttt tgtccttcca aattttatgc gattttcaga
    31261 tgcttttatt tctttttcta agcggacttt agcctttctt tttttattaa gattaatgat
    31321 gtctgccatg gcactctctg ttgtaagacc ttgtttatac tatagcgcat aaagccaggg
    31381 caaatcatct caatacacaa atcgatcgaa atggaatgat ctgaaagtat acactttgag
    31441 aatgggaagg actgtcgcaa gatagtattt tcagcattgt tttttatgac actttgcaaa
    31501 aagaacaagc atccttttaa gtgcaggatt ttagtgtata accagagtgt attcacagag
    31561 ttatccacag aaaatgggga taagtaatca atacaattca cgtattgata tttaataaat
    31621 ctttacaggg caaggggttt tataaaaaag gcatggtgtc aaatactgat gagattaaaa
    31681 ataaagacaa gttatccaca gaaagcggga tgctagttaa ggtatgggac gctcattatg
    31741 ataacaaaaa aaaaaataaa aaacagtctt gactggcaca attttttacc tgaaaaagat
    31801 ttggatagac tggcatcagg aataaggcaa tacaaaaaca gctaatcctg ctataatttt
    31861 tgaacaaaac gtgagctcct gttggcagga tttttcactt tagatcgttc actgacggct
    31921 tctgaggctg atggcataga gggggttcag gtgattttct catgagtcga gttttcaaag
    31981 gagtatgaaa tgagtaacta cctcgataaa accgcaagct taaccccagc cactaaaaga
    32041 atagtgtgca acaaagctac tgaatatcct catactggct cttataatca agtagcgact
    32101 catggcacgt atttatgcag gcgctgtggc ctggcgttgt ttcgtggtgt cagtcaattt
    32161 agctcaggct gtggctggcc cagttttgac gatgaaatag ctaacgctgt ggcaaggaaa
    32221 ccggatgcgg atggtcagcg caccgagata ttatgcgctc gttgtgatgc tcacctgggg
    32281 catgtgttca caggggaata catgacgtat aagaatttac gtcattgcgt taattctgct
    32341 tcattggatt ttgtcgcgga taatgaagta ctggatacag aagaagccat ccttgcaggg
    32401 gggtgttttt ggggagtgga atattattta aaacgaattc ccggagtgtt aaaagtcgag
    32461 tcgggctata caggtggcca tgtttcagag ccaagttatg agcaagtatg cagtggtact
    32521 tcagggcatt atgaggctgt gcgagtcgtc tataattctg ctaaaaccaa ttatcatgac
    32581 attgtaaagc gattttttga aattcacgac ccaactcagg ccaatggaca aggtcccgac
    32641 ttgggccacc aatatttaag tgccgtgttt tattttaatc aggaacaact ccaggaagcg
    32701 gagttattga ttcagttatt aaagaaaaga gggtatcagg ttgtaaccca attattgcaa
    32761 gcacaaccct tttggcctgc tgaagactat catcaagatt actattttaa acaccacaaa
    32821 attccttatt gccataagcc agtcgatcgt tttggctagt tgttccggaa gatttaatct
    32881 catcatagtt gcacggattt agaaggcaga caactattgg catattcttc aagatcatct
    32941 atcgcgcacg tgttttcaaa ttcttctgca ctgcttgtct tcattttcaa gtctatggtg
    33001 tttctccatt tgagcgcttt cttcatgatt ctttcgttgc gctttatgtt ctctggtgtt
    33061 tggggttggt cttcaaaaaa actacagtgc tttagaagag agtgcttgaa ggtttcctct
    33121 ttagaattaa ttaatggggt agaattcagg actttatcat ctgtgatgat ggctcggctt
    33181 tgtgcaccgc gtaatatccg agggggcaaa ctggcacaat gcaccggaaa atcagcatca
    33241 agttttgcaa tttttacctt gtcacttacc tcagcttgtt tatccataaa atcataaata
    33301 ttttcatatt tatcctgggt agacaacatg ctggcgcaat ccatagtaaa ggtggcgcac
    33361 cctgcgcgcg agtgctgtaa tttaacatca gggatgtaga tttttattga aagatcttcg
    33421 ttttcatcaa gcaaatcctg gaatccaact tcattcgcta atatttgagc agtgttatag
    33481 gataattgtg ctcccaaact gtcaagcacc aaaattttta aagtattatt gcccttttca
    33541 atggaatatt gtagctcaat taatgaccaa tggcgcacac cggatttttc tctatccaga
    33601 taggcaattt gtaccttgaa attgttttca aataattttg gtgaatgaca actgctataa
    33661 ataaaatcat aaagcttatc acggcgttcg taatcacaga caaaaccggt aaatggataa
    33721 cctgattcct ttgattcccg atattttttc tttaaactaa aggccacttc caaatcatgt
    33781 gcggaaaatg cagggaattc gtctagagaa aatttatcaa cggttgtttc tgatttgcta
    33841 aaagccatat aaaaaatccc attcattttg tatttttatt atatgaaatg tttttccatt
    33901 attcaatttg tttcttgtcc atgtcgtaac tccaagaaaa tcatttaaca acaaaaatag
    33961 cttataatta taatgtacaa tatttttcaa ctgcataacg gatatcgatt tatgcaagga
    34021 gtgcgttatg aaaatattag tcaagtcatc cattttggca atcgttttgg cgctttcaac
    34081 accactattc gctgatgata cacaatggat gacagagcat aatttggtga aaggggttat
    34141 cacttttcaa gatgattcgc aaattcaggt ttatgctgct caagccgatc ttgataaatt
    34201 gcaacagaat cttgctaata ttcaatcggt tgaagtacaa cctaatcaaa ccgattctac
    34261 aacgtcacca aatcaacaga gtgaataaca cgggtcttgt ccgagttaaa cgacatgtgc
    34321 tgtttccagt ttagcttggg cgtgtgtttt tcttgtagtc agagttttgg ttttctgaat
    34381 ctgtctgctg atattgctga tcagcctttt ccgctttgcg ttggttttca tacagctgaa
    34441 tttcattgag ttgttgctgc caaaatgaag aagcatgctc ttttaattga ttgatttttc
    34501 gtacgagcat gccaaggtgg gttgccagca tgggaagctt tgatggccca aacacaatga
    34561 tagcaacaat gaaaataagt aataactcac caagactcat gatggtttat gatcgtcttt
    34621 ttgagtgtca tttgtttcct cactattcat ggctttgcgg aaattcttaa tcgcttcgcc
    34681 aaggtcagag cctattgttt ttaatttgga agtgccaaac aaggctacaa taatggctag
    34741 aattaataag agtgataacg gacttattcc acttaatccc atgttagttc cttattttgc
    34801 ggttgatgag tacaaaaatt ccagctaaaa cagaaccagc tatcgtgatg gttgtgagat
    34861 gattataatc cagcaaatca aaatagctaa tgacacctaa tgataaaaat gagaaaaaga
    34921 cacccagtcc cacactcttc cattggatgg tctgattgtt attttcagat tgcaagcgtg
    34981 tgaattcatg gactgttaac aactgatcct ttttcatctc cagtatatca aaaatcagtt
    35041 taggcatatg ggggagttgc tcagtaaaaa agggcaaatt ttgctttaag cgtttaatga
    35101 atgctttagg tcccatttgt tcgcgcaccc atttttcaag gaatggtttt gctgtagccc
    35161 ataaatccaa ttcaggatag agttgcctgc ccaaaccctc tatcgctaac agggtttttt
    35221 gcagcaaaat cagttggggc tgcacttcca tatgaaacct tctggcaacc tggaataaac
    35281 ggaaaactac ttgcccaaag gaaatgtctt ttaatggctt ttcaaaaata ggttcgcaaa
    35341 cggttcttat gctgctttca aattctgcaa caggggtgtc acgcgatacc catccggatt
    35401 ctacatgcaa ttcggctacg cgcttgtagt cacggttaaa aaaggcaacc aggttttcag
    35461 ccagataacg cttgtcatta tctgtcagag tcccaatgat gccaaaatca acacaaatat
    35521 actgagggtc tttgggattt tggtaggaga caaagatatt tcccgggtgc atgtctgcat
    35581 gaaaaaaaca atcccggaag acttgagtaa agaaaatttc tactccacgc tcggccagtt
    35641 ttttaatgtc aatgccgtga tctctaagac tgactatatc ggtcacgggt atgccatgga
    35701 tgcgttcaat gaccaggatg ttatgatggc aataatccca atagatttca ggtatatata
    35761 acatcggaga ttgattgaaa tttcttcgca attgagcggc atttgccgct tctctcatta
    35821 aatccagttc atccagtaag gtatgttcaa attccttgac tatctctttg ggtttgaagc
    35881 gctttccctc aggccaatag cggtcagtca atgcggcgat ggtgtacatg atacttaaat
    35941 cttgttctat aagccggcgc atattgggtc ttagtatttt tacaacgaca ttttcccctg
    36001 tcttcagagt tgccgcatgc acttgtgcca tggaagcgga agccagggcg atcggatcaa
    36061 attgggcaaa aacatcatag gcagactgtt tataggcctg ttcaatgatg ttcatggcga
    36121 tatggctggg gaatggagga actttgtctt gcagtttact taattctttt gctatgtctt
    36181 caggaagtat atcggggcgt gtggaaaggg cttgtccgaa tttaatgaaa atggggccga
    36241 gttcctccag cgatttacgc agcgcttcac cacgcgttaa tttctctttg cgaagccagt
    36301 tccatgggtt taaataaact ataaaacgta aaggagcaaa cagtttgata gacacaacaa
    36361 cattatcgag accatttttg gctaaaatat aattgatatg tataagacga ataagttgct
    36421 taattgattt cataacagct catcggttgt tgatttcgag cttccagccg ctcaatatgc
    36481 aatgataatc catccagatc ctgaataaaa tcatccaatc gtttaattgc cttcatcatt
    36541 actcattaaa ttattgatat gggcttgaag gcgatcaaca cttaacacca attcatcaac
    36601 ctcggcaaaa aaatcttcca attcatttct tgaaggagaa atgcgtaatt cttcctgaag
    36661 aaattcggtg atgttttgtt gcattgaggt gctgaattga tttttaaatt ccagtccttt
    36721 acgtacaaaa gagccaattt gatgagccac cacatcacct gtgaagtgag ccaggtgccc
    36781 ttcccaatca atatctattt cgtcaaatag tttctttaca ctttgcccca attcaatgtc
    36841 gcctgagatg cgtatcttgt cattaaataa agagcgggct ttcgatgtgg gtaataagct
    36901 caagcgaatc aatcctatcg gattgctgtg aatgattgta tcagggcgcc catcaatgcg
    36961 atggagcaat tgcatttcac tccctttaaa gcgaatgtaa aaattaacat ttaagggggt
    37021 aatgaccatt tcaagtgttt tgccatccag tttttgaatt ttttgcggca tctgctcatc
    37081 gagcttcatt gcctgattga tggctgtttg taatgctttc agcgagtatt cttttaacat
    37141 aatcaactca atatttgtag gcgatatgca gagcgactat accgccactt aaattatgat
    37201 aatgacaatc ctcaaatcca acccgttcaa tcattgcttt taatgtttcc tgatcaggat
    37261 gcattctgat tgattctgca aggtattgat agctggcttc atcgttagca acaaattttc
    37321 cgatttttgg taaaatgtta aatgaatacc aatcataaac aggcttaagt ccaggaaaaa
    37381 ctggtgttga aaattcaagc accattaatt tcccacctgg cttgcatact ctgtacatgg
    37441 attgcagcgc ttcgtcttta tcagtaacat ttctaagccc aaaccccata gtgatgcaat
    37501 gaaaactgtt atccgcaaaa gggaggcatt gcgcattacc ctgaacataa cgaatgtttt
    37561 taaataatcc ctcatcaagc aagcgatcgc ggccaacatg gagcatggct gcgttgatgt
    37621 cggcaagaac aacctgtccg gagtcgccca ctttttgcga taataaccgg gttaaatcac
    37681 cgctgccccc ggccaaatcc aatacgctct gcccaggtcg aacatgactt aattcaatgg
    37741 tatagcgttt ccagagatga tgaattccca aggacatcag atcattcata cgatcataat
    37801 ttttagcaac agaatgaaat acttctgcta ctttcttttc tttctcattc cagtctactg
    37861 atttaaaacc aaaatgggtg gtttgtttct gattcgtcat atttgccgga tagtatcatt
    37921 aaagtgttca tcttaaccga taatcattaa gatacccagt taaaaattgt atactttttt
    37981 ttatccatgt acagatgaaa acaggggtat ttccaggata cacgctacaa attttgcaag
    38041 gttattaaat agtggttttc atctggcggc ctattgtttt tttgggaaag ccataagcat
    38101 tctgccaatt gatccatgat taaatgttca acagccatgg gatcctggta tttttctatt
    38161 aacttggtgt aaacagtacg gatgccttgt ggcctatcgg tcgttacttg ttcgcgaacg
    38221 gcaagatgaa gccccatatg aaggaaggga ttggtttcgc ctaattcagg ataataagta
    38281 tgttcaggaa aagattgaat ttgttcaatg actttatggt attccggatg atcaagaatc
    38341 acttgggcaa tttctttttc caagggagaa agttcttttt tattctggta cttattccag
    38401 ctgataaaaa atagctgtcg agtttcttgt actgtatcgc cgtaaaacat aatggcccga
    38461 ttgatataaa atgatccatt ttaactgaat aaaaaagtaa caacaatgtt gatgtgcaaa
    38521 tattgcaggt caagagcagg ttatgatgaa taagttttga atatttagtt cagccattat
    38581 caaattcacc catgaaaact aatacaatag gtgcataatg agctttatag attattttga
    38641 tgaaatcaga gaccttcgta aggataccaa tgttaaaaga taacttgatt gctttaaacc
    38701 cgatgagata aacctcaccg ggttagtgcg acttagaaaa agaaaatccc ttatgattgc
    38761 ggacaaattg ttccgggtgc gcagtttgta cacgcaatgg agcacaagcc caaccactcg
    38821 atcgttgcga gcctgtttat cgtctcttgg attaaagaga tcgttcggcg tggataagtc
    38881 tcatgcgcct ggatcttcat aagtgactga tattgtgcca agggtttagt ggtgccgggc
    38941 gctgccaaat caattgccac gagctccttg tttgtgtaag tctgcgcagc gtaaaacccc
    39001 ggttctagcg agattgcggg ataagcatct gttacgattg cttggcttac gtaaaaacct
    39061 gcaccaccat tcgccgctgg ttttccaaga ccatcaaagt tggcaataat gttggtagct
    39121 ccattgacct caatggtatc ggtcacaaca tagcggctag agttatcaag aaagcaccac
    39181 caaacaggag aagttggata gggattcatt ccgggctgtg tgctccagaa gctgtagcta
    39241 agataagttg ccagccattg ccccattttc atcccctcaa gattgcttgc cccaagatcg
    39301 tctgtgctgt gtgagcctcc aggtgaaagc gtattgcagg catgtcgttg tagtcgttca
    39361 gcagatggta cttgggcaga cggatagcga ctatgaatga aattcacaaa gcccgtaaac
    39421 tcttgctgaa agttcgcgtc agtaatacag gcttgtcgtg agaagcaaga cggtgaaggt
    39481 accgccagac ttttgctcat agaattgcca ggattgataa agagagaatt taaaacagac
    39541 atattcgatg ccttttgcga cattgctcca gtcggtgacg ccacaagaag aagcgataag
    39601 gctgcgagac agatgtttac aataggcctt ttcttgccaa aattgacaat cgctgcaaca
    39661 ttaggacgtc cagtgaacct aataccaaga aattctatca tgactattta actcccttta
    39721 attattctct attgattgat tgttgtttga gagtcgaaag ttctctggtt agacctatct
    39781 ggtagttgtt taccgaaata acccacaaac tgattgatga taacttgttc tttaggtgga
    39841 attcttgtcg aaaaataaat tcgcctagca agcaaataga tcacatggga ttatggaatg
    39901 aatggttagc actcgtcaat cactttgagg cggcttttag cagggagata aaatgcatgg
    39961 tgctgattca tcagtatcac actgtatgcc atttattcag caagttttat agagcaagga
    40021 ttgatcgttt cagtggtatc ataaacttaa aaattgaggt aacaaatagg gatttttctg
    40081 agcataggtg cttagaaaaa ttgctttggt ttatcatcaa tgttaagttg gcgatgccat
    40141 tcaatgcaaa gccactaaga gttaacaaag ttaagaggaa ggcttctttg atttactcca
    40201 tcagtactga ggtaaacatt aatgatgaaa acctttatca ttgcagcgga cagaacggtt
    40261 gattcttttg gttctcatca agacactgat aatattctta atctggccag ggagaaagga
    40321 cttgttgtcg taactttaga aatcttaagt cttgctcagg gatggcagga taaaatgaaa
    40381 ccgcatcagt tcaaaagtgg cgcatctgcc atggcagcta ttgagagagc tcggaaaatt
    40441 ctttccacca aaaaagcaga tgccgtcctt atcaagggtc aggatttttt aaaaactggg
    40501 tatggttctt gtgagcggga aaaatttatg aaattttatc atgagaatta caccccgctt
    40561 caagggtata acgaactggt tcctctcttt ttacaaaagt acaaactcga tgagaaagaa
    40621 tatttcaaga ttcgtgatgc gttgtttgaa aactacacga agacctggaa gaaaattaat
    40681 ccagcaaata gtatgcctca tgaaaaatgg tatcaacctc tcactaagta tttcaggggc
    40741 gtcgattgtg ctaatccgag tgttgactat tcaggtcaga tcatactatc gacggaaaaa
    40801 gtagctgatg ttttaaatat tcctcaaaaa gcacgtgttg aaattttagg taactcgttt
    40861 gtaaaaatta atctcaaggg tttagcttct ttgcctaaga tcgcaccgta ccatcacctt
    40921 aaacgagcca tcaacaaagc catcactcaa gccaagctca attttaaaga caaattcatg
    40981 aatgaaaaag ctttacttga cgcatacacc tgcttcccga tcgtacctat ggctctcctc
    41041 tattgcctgg ggttagtgaa ctctttgact gaaattcctg acttacttaa gtcatatgaa
    41101 gtaacagtaa ctggaggtct gaatctcggg aaagcgccct ggagtttgac atctcttaat
    41161 gcgctcattg tgatgcggga gaggctaatc tcatcagata aggcgaaatt tggacttgtg
    41221 catgggaatg gcttatttgg aaaccagcag ggaataacta ttataaaaaa atgagcctcc
    41281 ttaaatcttg ttgctttagc aaatatggta aaaatgttga cattaattag tggagtatga
    41341 gatatttatt ttgcgaggtt ggacttgcta tgtgtcattc cactgggtca attggcgaca
    41401 cactgattgg ccctttctat tatcctgaaa tcctgacaag agctctctat ggcttaatct
    41461 ataagctgct tgtgattaat ttcatcgcaa tataagccat taaaataccg ctaagtaact
    41521 ctattttttg ccatacttta ttttgagtta acaggtttga aaaataacga gtagtcatcg
    41581 ttaatgaact gaaccaaatc atgctggcag aaattacccc tgctaaaaaa gccagtttat
    41641 ggtcaggata ttgactgctg ccgctaccca caatcaccag ggtgtctata atggcgtgag
    41701 gattaagcag actaaacccc agggcaaata aaatgatttg cattcttgta tgaggctgat
    41761 gtgtttctac aacggtttgt ttgtttttgg acagcgcgct ttttaagttt tttattgcat
    41821 aataaattaa aaaggcagag cctagccata ccatccagat ttgcaagttt ggatgagcca
    41881 aaagcaattg atgtaaacca gcgacactgc cgcacaccaa aatgacatca cagaaaaagc
    41941 aaatgacggc agagagagcg gcatgatttt ttcgcgcacc ttgcctgata agaaagacat
    42001 tttgcggacc taaggccatt atcaaagata atcccaagag taatccatta aaataaatca
    42061 acataattgc atcaggatag taaaaaaaaa ggcgattata tcataatttt gtgcaagatg
    42121 ttttattgtt gattggtgtc gcatattttc tccaggactt tatgtaacat gggagcgact
    42181 tttgtgctgg agtaatgttg cactctctga aatgctttga gtgcaagttg ttttgacagg
    42241 tctttgtctc gcaagaggcg ttccaaacca tccgccatgc tgacagggtc ctctggggga
    42301 acaaagagag cacagcctga gttacctgca atttcaagtg ggcctgataa acgagtgaga
    42361 accatgggca aggaatgcat catgctttcc agtacaacca ggccaaaaga ttcttcccga
    42421 gaaggcaaac agaaaatatc aatactttta taaaatgact ctttatcctc aatccagcca
    42481 agtaaactga tttcatgatc caagccatga tgacgaatta attcgatata atgctctttc
    42541 tccttgccgt caccggcaat ttttgcttga aaagcaacac ctctcttttt tagttcagcc
    42601 aaagcgttta taaagacatc tacccctttg attgcagcaa atcgggcaca aacaccaatt
    42661 gttgggatct ggttgttttt aggttctctg tattgcaggc tgtctggaat atgaatcaaa
    42721 ttgggaatgg taaatacccg gtgctcctct atccccgaat tgataatatc ccgacgcatt
    42781 tgctctgtga gtgcaataat cgcatcggta ccaaagtcat agtggccttt gacatggcac
    42841 acagctatct tggggacttt ggtcccggtt tttttgaaca aataggctgc tctgtaacta
    42901 tgggtaatga tgcaatccgg ctgatggatt ttaattaatt ttttcaggcg atgaattgca
    42961 aaaaaatcat gctgattgaa attatgcacg gtgattaaat ggtcttgggg gcaactgttc
    43021 cttatttctg ctttgggatg aataattgga atgaccagat tgccttgaga acttaaagct
    43081 ggaatatagt ttaaaaatac ttgttccagg cctccgttta ccttggagaa catcgcatta
    43141 aatattttca ctttaaattt tcctcattca gagctgttcc tctattgata tagagggggg
    43201 gtagcaatgg gaatggctag cccacatcaa ccaaaacttg cccattttct atctgggttg
    43261 gatatatttt taaatttttc cgggaggaaa ttttacctaa aagattgcca acaacaggag
    43321 gccagggaga ccagcatttc gcttcgccag ttttcaaatc aaattcgctt ttatgaaaag
    43381 ggcaaatgat agaacaggat tccgtaattt ttcccttggc cagtggcaat tttaaatgag
    43441 ggcactgtga ctggatggca tgtacttgat tatcatgcca gataatcagt attttttggc
    43501 catcaataac tgcgacttct ctgttatttt gctgcaactt gactaattct acggcaggtt
    43561 tgaaagacac gttaattacc tctatattga aaatcagagg attatatcag tgatgaattt
    43621 aatctgttag tagaaggctg ataaaataaa tcagttgtgt tatcaaaatt ccttattttt
    43681 gctgagttag cccagggtat cgtatcaatg accatgccgg aatgatgtct ttgagcctga
    43741 ttatggcatt tcatgaaata atgaaatttc gagaaaaggt tgtgagatat aaggcagctg
    43801 acagctgggt tggcataatt ataatttttg aacagggctg gatttaaacg gtcgaataac
    43861 gttgcaactt gataagcgag gagtatacaa tgcgacaatt attctgtctc ctcatgagag
    43921 tcgtgaaaaa taagaacaaa taggagggag cgtgggctct ttggttataa aaaaatctat
    43981 tattgcggcg ctagttgttg cactgggtat tgcagggcaa tctgtacaag ctgagtctgt
    44041 accagatgaa aagttacaat ctgaaaaagt acagacggaa aaagttcagg ccgaaaaaac
    44101 agaaacagat ttgttttctc ccgtagagca aatcatgaat cctttttatc ccaaataccc
    44161 cccgaccaag ccagctactg gcgagcaagc agagttgatt aaacgtggtg agtatctggc
    44221 aaaaatgggt gattgcattg cctgccatac cgacgttgaa gggggtaccc ccgcttatgc
    44281 cggtggttta cctattaata ctccttttgg cttattctat agccctaata ttacccccga
    44341 taaagaaaca ggtataggaa attggacaga aaaagatttt attcgcgctc ttaaagatgg
    44401 tcgtgatcct aaaggcagaa attattttcc agtattccca tatgtctatt tttccaagat
    44461 aactgacgat gatgctcgcg ctctgtatgc ttattttatg agcattcctc ctgttaagtt
    44521 aaagaacaaa tcattgccat ttccttttgg tgtccccggg gctcgctttg ctttatgggg
    44581 atggaacctg ttgtttttct tccctcagga aaattccatt gaatatgatc cggataaatc
    44641 tcctgaatgg aatcgaggaa ggtatatagt cgatacttta gggcattgca gtatgtgcca
    44701 tacacccatg aatatatttg gatcagcaaa aaacagatat tatttgactg gtagttttat
    44761 cgacggatat tgggctccta atatcaccaa atatggattg gaatcagcga cgcatcaaga
    44821 ggtggctgat gtatttaagg ctggtgaatt aattaataaa gcgggtcctg tagctggtcc
    44881 tatggccgaa gtgaatcata atagcttgag tcatttgaca gaagcagaca ggttggctat
    44941 tgcgacttat ctcaagacag tagagagcga agaatacctg ggtgtttctc cttctgattt
    45001 gcctcctacc attgcacgtg gaagacaagt gtatttgaag gcctgtctga tttgtcatca
    45061 ggacaataaa atgggtgctc cagtgattgg tgattcagcc agctggtata cacgtctgaa
    45121 aagcagcggt ttaactggtt tatatcgaca tgccatttat ggttataacc aaatgccggt
    45181 taagggtgct tgcgtgacct gctctgataa tgacattatt tccgcagtcg attatttgct
    45241 caatgcgtca ttgacccgat ctcagtggca agatttggcg tctggcggtg ttaaaaaata
    45301 ccctgccagt ggaaaggctg tatataatga aaactgtgct gtttgccata atgagggcaa
    45361 attgggtgct cctaaaatag gcgataaagt gacctggaag ccgctgatag cacaaaatat
    45421 ggatgttttg atcgaaaaca caatcagagg gaaaaatcat ttgccaaatg gtggttgcaa
    45481 tcattgttcg acagatgaag taattgaagc gatcaaatac atggtcagtc aatctaaaac
    45541 cgaaggtaat tattctttgt ggtagggtta ccagcaacat ttgaataagt gaactagctt
    45601 aaacattagt cggtttcccg gctacttgaa gagatgaggg attgggatgt taaacaggtt
    45661 aaatatctgt aaattaattg cgttatttag tgccttgact tctgcgcagt cactgtgggc
    45721 tgctgcggat gagtggcagt taaacatgta caaaggggtg actcctttaa gcaacgatat
    45781 gtattacctt cacatggtcg ctatggctgt ttgtgcggta ataggagtgg ttgtttttgg
    45841 cgtgatgatc tactcattaa tccatcacag aaagtcaaaa ggatatcagc cagcaagttt
    45901 tcatgataat ccaaaattgg aaattatctg gactattatc ccgtttttaa tccttgtcgg
    45961 tcttgcagtt ccagctaccc gagtattaat ccgtatggat gatacgcagc tgtccgatgt
    46021 gacggtcaag gtggtcggct atcagtggaa gtggcagtac caatatcttg atcagggtat
    46081 tagttacttc agtagcttat caacgcccta tgagcaaatt gaaaacaagc aaaagaaaaa
    46141 tcagtggtac ttgctggaag tagataatcc tttagtcgta ccagtaaaca aaaaaattcg
    46201 atttttagtg acatcgaatg atgtagtcca ctcatggtgg gtgcctgagc tgggcgtcaa
    46261 gcgagacgct atcccgggat ttatgcatga ggcatgggcc agaattgaaa agcctggtgt
    46321 atacagaggt caatgtgccg agctttgcgg aattaatcat ggttttatgc ctatcgtagt
    46381 gaaagcagtt agtgaagaag aattcgatcg atgggttagt gagcaaacca aagtagaaga
    46441 catttatgct gaaaagtcgg ataaaccgcg tgaacaaaaa acaatgaccc gtgatcagtt
    46501 aatgacgctt ggcaagcaaa aatacgatct gatttgctca gcttgtcatc agcctaatgg
    46561 cactggcttg ccaccaatgt accctcctct caagggtagt tctgtcgccg ttggaaagcc
    46621 aatttcacga cacattgaat tactcctcaa tggtgttcct ggctcagcaa tgcagtctta
    46681 taaagatcaa ctgactgatg aggaaatagc agcaattgtt acgtatgagc gtaatgcttg
    46741 gggaaataat actaatgatt taattcaacc cgcagatgtt gcggcactgc gacatggtac
    46801 cgatattaaa cctaccatgg ttaataaagc taaagctgga ggtttgcaat gaaatacgca
    46861 atagcgcatt cagaagattc tcatgaagat catggtcccg aacaaggcaa aggctttatg
    46921 ggatttgtca aaaggtggct atttacaacc aaccacaaag acataggtac tctgtattta
    46981 tggttggcat tgataagctt ctttgttgca ggagcaatgg ctttggttat caggcttgag
    47041 ttatttcaac ctggccatcg attcgtcgat cctaatttct ttaaccaaat gacaacattg
    47101 catggattag tcatgctgtt tggtgtcgtc atgccagcgt ttaccggtat ggcaaactgg
    47161 caaatcccca tgatgatagg tgctcccgat atggctttgc caagactaaa caactggagc
    47221 ttctggattt taccatttgc tttttgtctg ctgttttcta ccatgtttca tgccggtggc
    47281 ggcccaaatt ttggctggac catgtacgcg cctctctcta caacttatgc accacccagt
    47341 actgatttca tgattttttc cattcatatg atgggactct cgtctattat gggatcaatt
    47401 aatatcatag ccacgatttt aaacttgagg gctccaggta tgaccctgat gaagatgcct
    47461 ctattcgtct ggacctggct tattacggca ttccttctga ttgccatcat gcctgttctg
    47521 gcgggtgcgg ttactatgat gttggccgat agacactttg gcacttcttt ctttaatgcg
    47581 gcaggcggag gagatcctat cctgtttcaa cacgtgtttt ggttctttgg tcatcctgaa
    47641 gtgtatgttc tggtcttgcc agcttttggg gttatttcag aaatcattcc cacattcagc
    47701 cgtaaaccat tatttggtta tcattttatg gtttatgcca cagtgagcat cgctgtatta
    47761 tcctttatag tttgggctca ccatatgttc accacgggaa ttcccctggg agctgaatta
    47821 ttctttatgt acaccaccat gctgattgcg gtacctaccg gtatcaaggt gttcaactgg
    47881 gtgagtacaa tgttcaaagg ggctatgacc tttgaaacgc caatgctgtt tgccatagcg
    47941 tttgtatttt tattcactat aggtggattt acaggattaa tgttggcatt ggtaccggcc
    48001 gattttcaat atcaggacag ctattttgtt gtggctcact tccattatgt tcttgtgccc
    48061 ggcgttatct ttgctctggt agcagcgact tattattggt tacccaagtg gacaggacat
    48121 atgtacaatg agcgtttggg taaatggcat ttttggcttt ctgtcatttc agttaatttg
    48181 acttttttcc ccatgcattt tctggggcta gctggtatgc caaggcgaat ccctgactat
    48241 gctttgcagt ttgccaattt taatatgatt gtaacagtgg ctgcttttgc tttcggtttg
    48301 tcgcaattgt tgttcctgta caatgtggta gccacagtaa gaaaaagagg cactaataag
    48361 gatgtagcaa cgagcagggt ttgggaaggg tcacatggat tggaatggac tttgccatct
    48421 ccaccacctt accatacatt taccactcca cctgagatta aataaaggtg gagtttgatg
    48481 attgaagaaa aaaaacagaa aaaactgctc accatattaa cattattagt attagggatg
    48541 tttgcttttg gttttgctct ggtccctatt tataatagcc tttgcaagaa tctgggcatt
    48601 aacggtaaaa ccaaaatgga ggctgttgct tatgatgaag ataaggcaaa aattgcagca
    48661 gatagagaag tcttggttga attcgtagca actaataaca gtggtgtgcc ttgggccttc
    48721 tatcccaaga caaaaaaaat taaagtgcat ccaggagaaa ttgtaaagct ggcattctat
    48781 gcggaaaata aaacagacca tacaatgaca gttcaggcaa tcccaagtgt gacaccagga
    48841 attgcagcaa aatacttaaa aaaaacggaa tgtttttgtt ttactcaaca aacgctaaat
    48901 ggccatgagg caatgaatat gcctttacta ttccatttag atacggattt accgaaaaat
    48961 gtgaatacgg ttactctgtc ttatacattg tttgatgtga caggcagatc aagtaattag
    49021 gcgtcgttga tatgtctgcc tttgcggctg gcttgcctcg attttctgaa cgttgtttct
    49081 tgaaatcgtg acaatgcaat tcacgctagc caaatatcaa cggtctttga gtatcagctt
    49141 tggctgaaag acttattgtt acaggagatt aaagcataat gggagcacac ggtacatatt
    49201 atattcccaa acccagtcac tggcctttgg ttggctcaat aggcttgacc actacactgg
    49261 ttggtgcagc atgctggcta catcatgact ggtatggccc ttacatattt atagcgggct
    49321 tatgtgtttt ggttgcaatg atgtttggct ggttcggaca agtcatctat gaaaacagca
    49381 aaggcctata tgatttgcaa gttgatcgtt catttcgctg gggtatgtgc tggttcatat
    49441 tttctgaagt atgctttttc ggagcctttt tcggagccct gttttttgca agatattggt
    49501 cagtccctct tttgggtggt gagatgcatc ctattaccca ttacacctta tggcctgagt
    49561 tcaaagcagt ttggcccttg ctgagtaatc caaataatca gacttttgcc ggggctcatg
    49621 aagccatggg ggcatggggt ttggctgcaa taaacaccat tattttatta acttccgggg
    49681 taaccatcac ctgggcacat tgggctttaa aattaagaaa gcggggccaa ttactgacgg
    49741 gtatgtcatt gaccatcgct ttaggcgttt tgttcttgat gttacaaagc tatgagtacc
    49801 atgaagcata tacggaaatg aatttaacgc tggatgcagg tatttatggc actacctttt
    49861 ttatgcttac aggattccat ggtttgcacg taacaatcgg aacgatcatg cttatcgtta
    49921 ttctggttcg ttgcaagagg ggtcatttta ctcctgaacg gcattttgct ttcgaagggg
    49981 ttgcgtggta ttggcacttt gttgatgtgg tgtggttgtt cctatttgtc tttgtctatt
    50041 ggctatagac ttaatctaat tcatcctata ttacaaccgg gtgttgataa gcaagcccgg
    50101 ttgaaattga atacgaacga tttccatttt gtaagtaaag cagtaatctg agaactccaa
    50161 ttcacgaacg atggcatggc ctcgaaaata ttttgcaata aaagtaacct gactttcttt
    50221 ttcaaaataa tcattacttt ttaattccaa ttccgattca gacaagctaa tcatttggca
    50281 ttcaaagtta ttttgaccaa tcttaatggt tataacaaca ggttctatag gttgaattag
    50341 cacagtttct ccttaactga gcggcttgat ttgcttgtct taatagtgac tgacattaga
    50401 taatgaattg tttctaattc taaaactcac tctgcactat ttatgtgtaa tctaaatgaa
    50461 ataatactcc tcctatttgt attctagacc atcaatgatc aaattgttct taatttaaag
    50521 atattgtctt gaatgggttc ttgtgcacaa gaggtattag atggtaagtg gaatctatgt
    50581 tttttgccca gtttttagtc gtccaggact ccatctgtga tgcttttgat ggcattctgg
    50641 catatgccat ggctctgtgg ttattccaag ctaat
//
LOCUS       DAXLDT010000006        50209 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXLDT010000006 DAXLDT010000000
VERSION     DAXLDT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 50209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50209
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.006"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            188..334
                     /locus_tag="ACF2SP_000273"
     CDS             188..334
                     /locus_tag="ACF2SP_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358088.1"
                     /translation="MKQSHWMVLACCFILTACMSSGTSSKNTKSLEEHVAQGHVGHGG
                     GGGR"
     gene            473..3163
                     /locus_tag="ACF2SP_000274"
     CDS             473..3163
                     /locus_tag="ACF2SP_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFD2358089.1"
                     /translation="MAEQWHEKSPADMALLFETDLSLGLSEKEAQQRLKEVGLNLLSQ
                     QKKTSPFLIFLQQFASVVTWVLLGAVVVSFLLDEKADAIAILAIVILNAIIGFVLEYR
                     ADRAMLALQQMAAPKATVLRDGRVRVIAASDIVPGDILLFESGDLIASDARLFELSAL
                     KVNEAPLTGESIPVAKNLEICAKETPLADRKNMVFMGTSIADGTGRALVVATGMQTEM
                     GHIAKMLGEASRDETPLQKKLNQVGSRLLWLCFFIIIAIFGLGLLRNIPIFSLFMSSV
                     SLAVAAIPEGLPAVVTVALALGVQRMVRRAVLVRRLSAVETLGCLQVICTDKTGTLTV
                     GEMTARKLVTASDVYSIQGEGYNLTGGFSLQGQEINVSDDTLLQATLRAMTVCNNAEF
                     SEQNGQTTTVGDPTEVALLVAAAKGGVWQGELRSSYPRIKELPFSSERKRMTVVCRQD
                     HQNIAFVKGAPELILERCTYILTKTGIKKLTPNDKARMKQSCELMASEALRLLAFAER
                     QIEPIALEKEEEEIENNLVFLGLIGLQDPPHASSKESISRCKKAGIKPVMITGDHPDT
                     AKAIAKELGILEAGDRLLTGNELENMSEEEFNHCVKDIAVYARVTAEHKLKIVRAWKK
                     QQMVVAMTGDGVNDAPALKEASVGIAMGKTGTAVTKEASDIIVMDNNFTSIVAGIEEG
                     RTIYDNIAKTLGFLLAGNSGELLVVFVALLIGWPLPLLPIQLLWINLVTDGLPAIGLA
                     TDMSEPGILNRPPRATQKSMMDMAFFKRVTFVGCLTALVTLGVFAYEYLIDKDLVQAQ
                     DAAFSVLVTAELLRAFGARSDTKNIWQVGLFSNLRLFFIVSISFSLQVLIHHIPALRE
                     LFGIEPVSLTQCVVWILLGMVPLLVIEAQKRLRKAPNEAF"
     gene            3150..4409
                     /locus_tag="ACF2SP_000275"
     CDS             3150..4409
                     /locus_tag="ACF2SP_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2358090.1"
                     /translation="MKHFSVKKMIWTILLGFSCFTGPVSYAKIPPALSQLINEATQNN
                     PQIRAARDRLLAAIHVIPQARALPDPKLNAGYINMSENIPMDVDPRREQMLGGQQEIP
                     FPGKLIVRGRIATLEAKRAEAEYQATSFAVIAELKRLYYDLYFVNKSIEIVQRNQELL
                     HEMEKSSEANYSVGKTPQQDIYRAQTEISRLLMRLVILKQQRGSLQAGINRLLNRSLE
                     ITIHTPAVLPVTPMCHNLEYFYTLVKSRAPQLIMQQRAVQKGRQAIRLSKMEYFPDVE
                     IEGGRLHDTGMHTKGYQVLLKATVPLYFMQKQNHAVRESLARYNADIEDLQTTYRMLS
                     FQVKNAYLLAERSAKLIHLIQHTIIPQASLTFTSSQATYGVGKVDFLTMLNNLLTLQE
                     NELEWHGELAEHEKAIAQIEESTGTFL"
     gene            4406..5539
                     /locus_tag="ACF2SP_000276"
     CDS             4406..5539
                     /locus_tag="ACF2SP_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2358091.1"
                     /translation="MNFKMIIKGVVILSLLSLLQVTQAQQAMDNHDKMDMKQSTQGNE
                     IVIDPARLQAIGITSEPVRRQVVEKIIRTVGRVEADERRVAHIHVRFDGWIENLFINF
                     TGEKVKADQALFTVYSPELVATQQEYLLALNAQKILSKNTTSSAARGAQSAFQAAHQR
                     LLLWGISEKDIEQLRRTGKITRTMTIHSPIQGTVLKKMALVGMRIEPGDELYTIADLS
                     RLWVLGDIYEYELPYIRLGQMADLTLTYLPNELFKAKLDFIYPTIDMKTRTAKVRFEV
                     DNQKEQLKPGMYVNLELKVPLGERLVVPKNAVLLTGERAVVFIYHGNGKIEWRDVTLG
                     VRAGDLLEIIKGVKEGDQIITSANFLIDSESQLKAAMGGMQHQ"
     gene            5618..8761
                     /locus_tag="ACF2SP_000277"
     CDS             5618..8761
                     /locus_tag="ACF2SP_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HFD2358092.1"
                     /translation="MVERIIEFCARNRFIVLLFVLGFSFMGYIALKNTRMDALPDISD
                     TQVIVYTTWMGRSPDLMEDQVTYPIVTALLSAPKVVAVRGFSDFGFSYVYIIFEDGTD
                     IYWARSRVLEYMSQLAGKLPEGVTPQLGPDATPVGWIYQYALVDDNGKHNLAELRTFQ
                     DWYLRYWLRAVPGVSEVASVGGFVKQYQVNLDPVKVLAYNLSVPEIVEKIRMSNNDTG
                     GRVIEFSGVEYMIRGRGYIKSTEDIEKIAVGTNANGTPILLRDVATVQLGSDMRRGVV
                     DLNGQGEAVGGIVIMRFGEDVLQVIGRIKDKLKELASAIPEGIKMVPVYDRSNLIREA
                     ISTANWNLIEELVVVGLLIIVFLGHFRSALIPIITLPLAILISFIPIYFLHVGLNIMS
                     IGGIIVAVGDMVDAAIIMVDNAHKRLADWEAKGSPGDRTQVLIDSAKEVGPAIFSSLL
                     VIAISFMPVFTLEAQEGRLFSPLAYTKNLAIFMSALLAITLIPVLLPLLVRGRIIPEQ
                     KHPITRWMQAMYAPVLRLALRYRQFVVGIAIVLAMATIPLYKLIGSEFMPPLYEGTIL
                     YMPVTLPGISVTQATGLLQEMDKKLKAFPEVAHVFGKTGRAETSTDPSPFNMMEVIVE
                     LKPKEFWRKGMTYESLVAEMDEALQFPGVSNAWTMPIKARNDMLTTGIRTAVGIKVFG
                     PDIKKIEAIGKEIEMVAKEVKGTSTVYAERVAGGYFLDFQINRDQLARYGLTIMDVNR
                     IIESAVGGENIATTIEGRERYPVNVRYLRELRDDPDKLNRILVKTPSGAEVPVAQLVT
                     LTFRSGPAMVRDENGMLAGYVFIDISGRDIGGYVEELKQAVNAKVKLPPGYTLAWSGQ
                     YEFMQRVYERLKIFVPLTLAIIFVLFYFTFRTITETLMVMLGVPFALFGGFLLLYILG
                     YNMSIAVWVGMIALAGVAAETSAVMLAYLDSDYNEQKEKGLLKTLPDLIQLVQLCAVS
                     RIRPMAMAGLANIIGLLPVMWATGIGADVMKRLAAPMVGGVFSALLLTLIVIPVVYVM
                     WRWQVDLKKTADLTEAR"
     gene            8758..10119
                     /locus_tag="ACF2SP_000278"
     CDS             8758..10119
                     /locus_tag="ACF2SP_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HFD2358093.1"
                     /translation="MKLTFLGATETVTGSKYLLTIGSKKILIDCGLFQGYKELRLRNW
                     APLPIDPHDIDAVIITHAHIDHSGYLPLLVKNGFQGKIYTTPGTKALCSILLPDSGHL
                     QEEEARLANKYGFSKHQPALPLYTEKEARTALQYFETIDFDTPHQLFHDFSFEYHRAG
                     HIVGAAMVKIKTQKGSIVFTGDIGRPYDPVMKAPAFIQETDYLVMESTYGDRLHDATD
                     PLPQMAQVINQTVKRGGSVIIPAFAVGRAQSLLYFIYELKRQGQIPKDLPVFLDSPMA
                     IDATHLLCAYKEDHHLTAEQCRGLCHVATYVNTPEESKEIDRHHMPQIIIAASGMAQG
                     GRILHHLKAFAPNPKNTLLFTGFQAGGTRGARIVNGEKQVKIHGSFIPIQAKVVVMSS
                     TSAHADYLELLEWLTHLTNPPKKVFITHGEPHSAQSLKQKIEKQLGFSCTIPSYLQTE
                     ELW"
     gene            10116..11630
                     /locus_tag="ACF2SP_000279"
     CDS             10116..11630
                     /locus_tag="ACF2SP_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HFD2358094.1"
                     /translation="MSKKTSHGLRLKHLGIKTYHEAIIYMREDCHVCHSEGFEVQTRI
                     QVTLGQHSMIATLNVVTSELLQPGEAGLSDYAWESLHAKEGDEIQVSHPKPLESLSYV
                     HTKIYGKELSYEQMKIIIDDVLSGRLSDVQISAFLAASSAGRLTRTEIMKLTKAMIDS
                     GDRLSWSSPLVVDKHCVGGLPGNRTTLIVVPIVAAFGLMIPKTSSRAITSPAGTADTM
                     ETLAPVHLSPQKMRQVVEQENGCIVWGGAVSLSPADDVLIRVERAIDLDSEGQLVASI
                     LSKKIATGATHAVIDIPVGPTAKVRNQSMALLLKQLLEEVGNELGLVVHTLFTDGTQP
                     VGHGIGPSLEARDVLAVLQGLPDAPNDLRERALTLAGAALECSSKVPTGLGKSIATQL
                     LDSGQAFKKFQAICEAQGGMRELTKARFTYPVVAAKAGKVSLIDNRKLAKIAKLAGAP
                     KSKSAGIDLHSHVGESVEKGEPLFTIHSESSGELHYACDLLRDKQDIIILGENS"
     gene            11627..12538
                     /locus_tag="ACF2SP_000280"
     CDS             11627..12538
                     /locus_tag="ACF2SP_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFD2358095.1"
                     /translation="MKPLLCSLFDSSEIAGQLQKTLQVEVGKVTFHRFPDTEWYLKID
                     SEVKSRSIMVVDSLNQPDEKILPLLFFAKTAKELGAKKIGLIAPYLSYLRQDKRFHEG
                     EGITSRYFADILSTSFDWLMTIDPHLHRYHSLDEIYSIPTFVLHATSMIATWIKHHVP
                     KPILIGPDMESEQWVADIAEKGRFPYAILEKIRHGDKEVTISVPYIPELESSTLVLVD
                     DIISTARTMVETVKHLQQAGSKSIVCIGVHALFAEDAYSLLSAMKGVQVITCNTIHHV
                     TNAIDVSGLAVDVLVQNKLAALKSKRK"
     gene            12563..12730
                     /locus_tag="ACF2SP_000281"
     CDS             12563..12730
                     /locus_tag="ACF2SP_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358096.1"
                     /translation="MLKAFRMIAVLIGLIGGPFFLSSCDGAVKHGSQEVDGGYGGHSA
                     GGHGGHGGAGH"
     gene            12794..15004
                     /locus_tag="ACF2SP_000282"
     CDS             12794..15004
                     /locus_tag="ACF2SP_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HFD2358097.1"
                     /translation="MKHDHHQGHTHSGKGHACPHEHNSPKTQQASSKMEGPIVYTCPM
                     HPEIRQSAPGHCPLCGMALEPETVTVSEVVSPEYLDMRRRFWIALMLTIPVVILEMGG
                     HGLKHFISGNGSSWIQLLLATPVVLWGGWPFFKRGWQSLKTGQLNMFTLIAMGIGVAW
                     IYSMVAVLWPGVFPHAFRSQEGVVAVYFEAAAVITTLVLLGQVLELKAREQTGSAIRA
                     LLKLVPESAHRIKEDGSEEEVSLDNVAVGDLLRVRPGEKIPVDGEVQEGRSFVDESMV
                     TGEPIPVAKEASAKVIGATINQTGSFVMKALHVGSDTMLARIVQMVSDAQRSRAPIQR
                     LADTVSGWFVPAVILVAVLSFIVWALLGPQPALSYGLITAVSVLIIACPCALGLATPM
                     SIMVGVGKGAQSGVLIKNAEALERMEKVNTLVVDKTGTLTEGHPKLTRIVTDDFVEDN
                     ALALAAALEHQSEHPLANAIVHAAKEKGLSLGTVEAFEAPTGKGVVGQVDGHHVAIGN
                     ARLMQEHGSDNAPLFEKADELRGKGASVMFMAVDGKTVALLVVEDPIKSSTPETILEL
                     QQSGIEIVMLTGDSKKTAEAVAGTLGIKKVVAEIMPEDKSRIVSELKDKGLIVAMAGD
                     GVNDAPALAKADIGIAMGTGTDVAIESAGVTLLHGDLRGIAKARRLSENTMSNIRQNL
                     FFAFIYNVLGVPLAAGVLYPLTGLLLSPMIAAAAMALSSVSVIINALRLKRVTL"
     gene            15143..16024
                     /locus_tag="ACF2SP_000283"
     CDS             15143..16024
                     /locus_tag="ACF2SP_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFD2358098.1"
                     /translation="MVIPVVMIVFGIVFLAKGAYLINGLVQQGNHYRRLRFVSLSFIG
                     IFILSYIASIFYGLLVDPHCLNYFYGSVFLLAATYTYFSCHLVTKTIQKAELMADFKS
                     RYELLRQHAQYDSLTGCHNRETLFEVLNTRLQQIKMHGGWVIVLFIDMDKFKTVNDRY
                     GHDAGDNTLQTFGQLLTQRLRKDDIVARYGGDEFIAVMENISIDEAKKIAENLITVTR
                     QSFKEGILSELNLGCSIGITQMNPHSASVNTVIKEADLACYAAKQRKINGSVCVYSPM
                     LATTNHLIDTSIHPIAD"
     gene            16161..16535
                     /locus_tag="ACF2SP_000284"
     CDS             16161..16535
                     /locus_tag="ACF2SP_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFD2358099.1"
                     /translation="MTHYLMKRTLLVIIPAFLVGCSYNAKLTPEGEKVTILQSGQLSK
                     GCTFLGEVRAYDRNGMSQSYQSHEHLIKDERNILRNEAALLGANTVLVSQHQQTYEGN
                     PKNDFVADHWMVGRAYRCKIKQ"
     gene            16560..17873
                     /locus_tag="ACF2SP_000285"
     CDS             16560..17873
                     /locus_tag="ACF2SP_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2358100.1"
                     /translation="MEYHRAWRFSYRVVLLLLYASLATAGKAWTLQEITQMAFANNKD
                     LKAARYNVAIAKARLLQAGLWPNPSLNLSNNDDRLFNDEGEYSRSAGFNQAFPISGRI
                     AKQKTVARLDVLKAMAEIREAERQLSAKVANAFYAAMITERRTQQVNYLLRINQELVD
                     VIHNRYHAAEISKLDENAARIEYLRIGQEKHLLHSLRISQYALLNQLMGREANSPLSL
                     KMDFIAERTLLKLPRLKALALKNRPDRQSIALSIERANADRRLAKAERFADWTVGLGV
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLFNRNQGRILEASHTGTQAIMALRALNLSI
                     ETEVTSNYGQLKALQMSLRETQQVSLKLAVENVTLARDSYENGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKYLQVYVALCTAIGPGKTNGFCPYLAYQRNGDSK"
     gene            17870..18838
                     /locus_tag="ACF2SP_000286"
     CDS             17870..18838
                     /locus_tag="ACF2SP_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2358101.1"
                     /translation="MRWQQRMRRMALISLFLSLLFPIQGCFAHGEEIEVSEGGAKGPV
                     HLTPQQTKMLDIKVVEATNRPMAQMLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DNVKAGQRLATVQSRLVGNPPPSVAVTAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVIAQVYEEDLDKIKVGQEVNVHALSYPKRTFPGQVTLIEPNLDSLTRTVNVRITL
                     DNEEKLLKPGMFVRANVILARNEAALAVPNAAVLQADNESFVFVQNKDIYDRVVVQMG
                     AVDDEYSEIKDGLVPGDLVVTQGHRELYTLWLSGGSKPHSGQEEPH"
     gene            18839..22054
                     /locus_tag="ACF2SP_000287"
     CDS             18839..22054
                     /locus_tag="ACF2SP_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2358102.1"
                     /translation="MFTHLIAWSLHNRPLILALTIILCLLGGYTLKQMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLITYPLESAINGTPGVVSVRSKTSVGLSTITVVFDDQTD
                     IYRNRQLVNERIQQVVNRLPPGVESPIMLPVTSAVGWLVKYALVSRTESPETLRTISD
                     WTIRPRILALGGVASVVSLGGEVKQYQVRLIPERMLAYRISVEDVRQALTSANQNVPG
                     AFVHQAGTEFVVSTLGRIKTLKDIKKTLIVVRNGVPITMNNVANVAFGGEIKRGDGAY
                     NTQNAVIGTISKAYGADTVTTTEKVEKALAEIKKSLPHDVTLMTNVFRQTSFIESAIH
                     NLTRALLEGAVIVIAVLFVFLMNWRASFITFLSMPVSFVVGILVLHYFGIGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRINRQEAHPLPTIQVVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIAEHIFSPLAIAYIASVLGSLVVSITMVPALCYWLLVCGQEKQQDV
                     EVSLHALSNKERHYAVEKEGNHQAESETRFVLWLKKHFLTALQWSIAHCKMVLAFALS
                     AFVFALALLPFFGTSFLPEFHEGNFIVVMSTLPGTSLNESMRLGQQVQKALLRYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVLLNFDKDKSMRPDELLRRIRADLANIPGAVFNV
                     GQFIAHRMDEVLSGVRAQVAVKIFGDNLSTLNELGQSMETLLKSVPGVVDVNKEQQIK
                     VPQLVIQLDREKAARYGVNVGQISEDVQVLLNGVSVSSVLEGQRTFDLYLRMDKPGRD
                     SVKNIQNMLIDAHGVGQNTITQIPLRAVAEIALEPQPFAINRENVQRLLVISFNVEGR
                     DLGSVIAEVQQEVQEKIKLPTGYFIQYGGQFESQQQASRVILVFGGLVIFVMLILLHK
                     AFGTFREALLVMFNLPLALIGGVISLFVVSGEMSVAAMIGFITLFGIAARNGIILVSH
                     YNQLRLQGKTREQVVIDGTMDRLVPVLMTAATAALGLIPLLWGSPAGKELERPLAQVL
                     LGGLLTSTVLNMLVVPTVYNAIEVWRKKRMQFNETEQGEIT"
     gene            22051..22491
                     /locus_tag="ACF2SP_000288"
     CDS             22051..22491
                     /locus_tag="ACF2SP_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFD2358103.1"
                     /translation="MKHITLKQKNHFWLGLIASVLMVFGSSQVFANPTESIPKTVPAI
                     WEAIDKHSASINQILGGDDLTSIHEHAFAIRDLANALPALSKDLSEEQKKTLQQNLSY
                     VGQLATRLDKTGDANDKEGTQANWQKLQKVLAQLRALYTPNAPN"
     gene            22599..24278
                     /locus_tag="ACF2SP_000289"
     CDS             22599..24278
                     /locus_tag="ACF2SP_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HFD2358104.1"
                     /translation="MDKENTDSPYQMGHDFISYQLDCWQRSILFWDTLRERANNMMEH
                     EQQGLPPLLNFKYELVLDGKSLEPKTNYALVKILEVGDVCFEECFDPHAHPVIIVDPR
                     SGHGPGIGGFKRDSEVGVALHSGHPVYFVIFYPNPVPHQTLADVLMTLRHFVEKVQAW
                     HEGKAPILYGNCQAGWMLALLASDCARSVGLTVMNGSPVSYWSNSEEEANPMQLLGGL
                     LGGSWSARFLSDLKEGIFDGAWLVSNFELLNPTTAIWDKYYGLFDEIDTEQERFLEFE
                     RWWNGFYQFSQEEIMATVNNLFIGNQLERGEMRIHKGCVYDLKRIQSPIVLFASQGDQ
                     ITPPRQALHWIRTIYPTTQALKKAKQRVVYLLHPSVGHLGIFVSAKVVRLHHRAILEH
                     SDAIEQLQPGLYEMIIVNPTGDPDCSKEQYHVRFEARELTELCTTSSTQQFDKVRQTS
                     EANDSIYQKVIQPSVQSLSNPFLSWCLEKTHPMRLSRYVFSEKVNPLMKAIELLSPPI
                     QANRRMATQSNCFKKIEHGVAQMMRDSLESSRIMRNDVMANWFEALYKDPD"
     gene            complement(24291..24443)
                     /locus_tag="ACF2SP_000290"
     CDS             complement(24291..24443)
                     /locus_tag="ACF2SP_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358105.1"
                     /translation="MNAKDCFVENDYEEKARETNPLFDEKGVHEADLESEMNLDEEFD
                     AIRSMN"
     gene            24632..25159
                     /locus_tag="ACF2SP_000291"
     CDS             24632..25159
                     /locus_tag="ACF2SP_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HFD2358106.1"
                     /translation="MLVPQTGIRNASTMIEIIPNWHPIFVHFTVALFTVSVILYALTY
                     VSSYIHWNTKPLIVELEIVARWCLWLAALSTMTTVSAGFYAFYTVKHGAMAHAVKVIH
                     RNWALATASVILLMAFWVVWRYIKHQKPTLLFLMALFLVQVLLLTTAWYGAELVYRHG
                     YGVLPVTAEKTVSPH"
     gene            25186..28272
                     /locus_tag="ACF2SP_000292"
     CDS             25186..28272
                     /locus_tag="ACF2SP_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HFD2358107.1"
                     /translation="MLQKDLRCSIRNYCFIFSFLLLGSSAVFAQHEQHGASTQPLTSS
                     KSTPSQSKPAQYKMEQPTTKKQSPKAVTPLTITGGAKRTVNLVVAYKTVNFAGKPRRA
                     IAVNGQIPAPTLHFKEGDDVTINVYNHLDEGTSIHWHGLLVPWQMDGVDGVSQKPIPP
                     GGVFHYRFKLYQRGTYWYHAHAKVQEQEGLYGTFIIDPPNPPSYHYTKDYVVVLSDWS
                     NTPAEQVLANLKKDGDYYGPRFPLQPSLMKFLHDYRKASSEERKKLIADYKMMQQMRM
                     SIYDLSDVAYDAYLLNGHPKSHPWTAPVKVGDRVRLRFIGAGASTIYRVKIPDAKVEM
                     VHIQGNDVTPYPIEDFWIAPGETYDILVTIQKNEPYIIYAESIDTLGKAYGALVTHPN
                     QVVNYQHVTPFPEPLPVTREMMANMMQSMNSGGMSGNQQHALMNKKTSSTAMKPSMTM
                     PSHSQAMSSQSANHSMSSKTIQKKMDNASNHSAHTSSMKSKSSSTSMNKSMDMPGMNH
                     GAMSQSKTPNSAQMKSDPSSTLMNKSMAMPGMNHSAMGQSKKSHSAQMKSDSSSTAMN
                     NSMAMPDMNHSAMSQSKTSHSANDMSMNRNMKSDRPMEHGMSMNDSMNMQMPIEPTII
                     GDKIEPPDSAKATTLGTKYQELKAAVKTNNPNKPVDGIIKMELFGYMDRYIWFINGLP
                     EYKAKPILIEPGKRYRIIFTNNSMMRHPMHIHGHWFILRNGHGAYDPLLHTIEVAPGA
                     TAVADFDTEASGQWFFHCHHLLHMTAGMARVFQYTTIIEIAKGTRKPDNYAYQQAYIN
                     RPVVREDEVMPLDASLIKHPAGHHQGFYRSSYIELGEDPFHNAQEMTFRGLYGPDYNK
                     LQLYTEDAEIYKGTVENADIDVFYWHLISQFWAVKGGVNYFYRPGGPYWQPGIGIEGL
                     MPWYIDTNIRTYYRDGSVKFDIQLARANQLTNNFFFLTGLRSILATHTVVKNEIGNGL
                     NQMRYILRPYYRLKPGLNIFTEYEHDEEYGALKRILRSQGEPTTQDTITLGVAVLF"
     gene            28462..29748
                     /locus_tag="ACF2SP_000293"
     CDS             28462..29748
                     /locus_tag="ACF2SP_000293"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFD2358108.1"
                     /translation="MYLDALSRLDKAASFCRIDSEALEKLKHPKSCLEVSLPVRMDNG
                     ELKIFTAYRVHHNDSRGPMKGGIRFHPKLDLDEIKTLALWMTIKCAVVDIPFGGAKGG
                     VIVDPKQLSRMELERLSRSYIELIADFIGPDKDIPAPDMYTNEMIMGWMMDEYATIVR
                     QNSPAVITGKPISLGGCLGREGATGLGAYYCIKILEKKKKWQSSELRVAVQGFGNAGQ
                     SIAKLLYDNGYKIVAISDSKGGIYNTKGIDIPRMIEIKNSSKEVQSIYCKESVCKLAK
                     DATITNEELLELDVDLLIPAAAQNQITQENAARIKAPIIIEIANGPITLEADALLQKK
                     GVLIVPDILANTGGVIVSYFEWVQNKSGYYWSMEKVQEELQTIISREFKNIWQLMEQY
                     QTDMRQAAYIHALSRYDKAVTAQGTRGYFSNLSLSL"
     gene            complement(29977..30300)
                     /locus_tag="ACF2SP_000294"
     CDS             complement(29977..30300)
                     /locus_tag="ACF2SP_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VrrB"
                     /protein_id="HFD2358109.1"
                     /translation="MWTLKTLKRFVAVAALIGSTAVAAAFDTSVKNDSQLGSMILAAS
                     VHHPPHWTPHGGHGKWHWDGHRWHWIQYHYKWHHNWHQNWHQQNWHHGGQGGHGWQGG
                     HGSGH"
     gene            30495..30788
                     /locus_tag="ACF2SP_000295"
     CDS             30495..30788
                     /locus_tag="ACF2SP_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HFD2358110.1"
                     /translation="MSPEPKHHPQSSKPQKEMHFWSSPSGMVAIIIIGIIGYFLIVEH
                     GAHIASFLGASPFLLLVLLCPLMHLFMHRGHGGHEEEHHHKSDNEDDSSEDKD"
     gene            30795..31460
                     /locus_tag="ACF2SP_000296"
     CDS             30795..31460
                     /locus_tag="ACF2SP_000296"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HFD2358111.1"
                     /translation="MEHTSYAYGLWLLVIVNSAIFILFAYSFTTTFKTRRDWRAFGAF
                     SAFVIAYFTEMYGFPLTIYFLSGWLSKYYPGVDLYGHDSGHLLHTLLGLKGDPHFDVF
                     HILSFVFIIGGLWMIASAWGTLYRAQKKHQLATSGLYAKIRHPQYDGFILVMIGFLLQ
                     WPTILTLIMFPILVYMYVRLARREEKEVLAEFGEEYQRYAAVTPGFIPRFGKQQDSKG
                     GAP"
     gene            31457..31957
                     /locus_tag="ACF2SP_000297"
     CDS             31457..31957
                     /locus_tag="ACF2SP_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358112.1"
                     /translation="MMFMFHSAEMLGLIAIALGIMLVVWALRNEGNSTGLSKVFGWLI
                     VIIGILGELCSSYYAIKYWYAGYFQMPMMSGEAFLFHVAQMLSLIAIAFGIILVVWTS
                     RHEGFGISLAKVFGWLIVLFAILGMICSAYYATVYWHKGYIQTPTGMPLLMQHQIPMQ
                     KQSPNQ"
     gene            complement(32023..32844)
                     /locus_tag="ACF2SP_000298"
     CDS             complement(32023..32844)
                     /locus_tag="ACF2SP_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358113.1"
                     /translation="MKLSWATLAALTVIAALSFMAYYLSSHGDEVSSLTNVAEWQRMA
                     SPGELSKAHSFLSHNCTACHTPVKGVEAANCNVCHANNESLLKRQPTSFHANIGSCVE
                     CHREHQGRTARITQMDHNALVSLGLQQLRAFSNANDEEEQLIINQVMLFLAQNESNKS
                     TLRVNPHSSPQELTLRCASCHQNKDKHFKLFGDDCSACHETAHWSLSEFRHPSPSSMD
                     CAQCHQAPPSHYMEHFNMISKKVAHEHEARVDQCFLCHQTTLWTDIKGVGFYKHH"
     gene            complement(32841..33602)
                     /locus_tag="ACF2SP_000299"
     CDS             complement(32841..33602)
                     /locus_tag="ACF2SP_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358114.1"
                     /translation="MNAQGRMMVLGLVLLMLITWLGFAVHTSSRFAGSFWGGVFGVSG
                     SILMLIPLLYLFVKRISFLKNWVTHYVSMNTLLSWHIYTGIIGSILVLIHTGHKFNSA
                     LGIALTAMTLIVTLSGFIGRYLMGFMTQEIDEKKTILLGLQQQYQIVAQELQASAHPK
                     QNIGKLHLMTARFLSWLLEDTALDGQLIKSAEVRALCLADAISELEYAIRFHEHFKRL
                     FQKWLTCHIITALVLYVLLGLHVWSGIYFGLRWFQ"
     gene            complement(33602..35485)
                     /locus_tag="ACF2SP_000300"
     CDS             complement(33602..35485)
                     /locus_tag="ACF2SP_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFD2358115.1"
                     /translation="MLFQYTKKFTLLRWVRTLSISFLLAMASVSFAQPTADEHAAHHP
                     GEKEKEGEGMSGMMKNIGTPSKELYPSLMSLPTLSPEKRLEIQQKAHQRITSGIALMK
                     GGLNELLLASSTNNYAKMQEALEKLREGIAQYESALAALRALSEGKNPSDIALQWFKR
                     EMNLLPTSPQAENVLGGPFFHLTIIALFALFFLVMIWMYFFKMRRAAALLDRLIQQNT
                     AAPLDTSPQFSKTPEPTPSLTSVEPIKSSQASKVPSETAPTTPPTCPAHKCPVPQFPV
                     MRSLTEPEEKWEGQLRVCRIFQEAPGIKTYHLASTHEVALPFTYYPGQFITLTALING
                     KTIRRSYTMASTPTQLHYCAITVKREEQGVFSRYLHDEIKEGDLLEVMGPNGKFTFTG
                     EEAKSIVLICGGVGITPMMSIIRYLTDIGWHNDIYLLYCCRTTSEFIFREELEQLQER
                     YLNLHVYASMLRSEGTVWMGLQGLFTKNIISHLVPDIASHRIHVCGPPAMMEAILGIL
                     KELNVPADLILTEAFGPEKKPEIVQEDFIKADTRAMVSFRKSEKMVPILPDRTLLEIA
                     EANGIAIDNACRTGQCGLCKVKLLSGEVTMACEDALSKEDKQQGLILACQAKATQNIE
                     VDA"
     gene            complement(35699..36568)
                     /locus_tag="ACF2SP_000301"
     CDS             complement(35699..36568)
                     /locus_tag="ACF2SP_000301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HFD2358116.1"
                     /translation="MTKRTKLKEHLHTLEEIGHIMTAMKNLSLIELGKITQCLSMQDN
                     VIKTISEVSHDFLSFYPMPPMNQQENPPLVSILIGSERGFCGSFNDNVLHQLDVHKEH
                     HSELEPALILVGHKLALKMPNDPRVIATIDGPNAIEEIPDVISNVLQTLEKALLQRNK
                     TWHSWQWTILFNEEEHNQIQAKTWQPFKELDTSPAPYFSVPPVLNGSKDQFFADLVEH
                     YLLAMCYSILYQSFFAENHQRLFHLNQALDRLENKKNALNHRLNLLRQEEITEEIQNI
                     VQSAEAIIGHELT"
     gene            complement(36626..38098)
                     /locus_tag="ACF2SP_000302"
     CDS             complement(36626..38098)
                     /locus_tag="ACF2SP_000302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFD2358117.1"
                     /translation="MNWPNTPSFLEKQRQRLERYQFQIKVSEQGRVVSVGDGIIWIKG
                     LPRAAIDEILISEDECCIAMVFHLTEDLVGAVMLVQTKKLKAGTPIFPLKRVLSIPVG
                     DKLLGRVIDPLGHPLDGGEIPPHEEQGLLDRLSPPILHRDFVNRPLYTGNKMLDNLIP
                     IGKGQRELLIGDNGLGKSALALDIVMNQKDKNVYCVYVLIGQKRSTVSSTIQLLKEAN
                     ALDYTTVVVAQATALPGLLYLAPFAGCAIAEHWMKKGLDTLVVYDDLSAHANSYRELS
                     LLLRRPPGREAFPADIFYLHSRLLERSTCLSPAMGGGSMTALPIIETKEGEMATYIPT
                     NLISITDGQIFFDESLFSSGFLPAIDITKSVSRVGGKAQHPQIKKESGRMKLDYLQFL
                     DLELFTRFGAKLDAKMQKQIQKGRILREILKQERFSPLPIEFQLAWLIAYNEGFFDEL
                     NLEDIPKILKKIEGEIKQSTLSLGNPREQWEKAIKEWLMA"
     gene            complement(38085..38828)
                     /locus_tag="ACF2SP_000303"
     CDS             complement(38085..38828)
                     /locus_tag="ACF2SP_000303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFD2358118.1"
                     /translation="MELSWTTFSLELINFLILIWILKRFLYEPIQKTILERKKRVQEQ
                     LETAQTLHRQATQLQTTYEHRLADWQQEKATLQNEWHEAMEQWKSEERLRFEKQLRQE
                     KEQVFSHEMQKAAATIENNAKEAFLLAGKFAEKLLMPFADAHLEENIIKKTIEDLNHF
                     PVEQWQWLNTVPEEETISIQTAYPIQEHQKQDLLQVIEQLSPKKLTVCFTENPKLLAG
                     LTLQMGPMCLQANLRDELKFFTETKNELA"
     gene            complement(38834..39109)
                     /locus_tag="ACF2SP_000304"
     CDS             complement(38834..39109)
                     /locus_tag="ACF2SP_000304"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFD2358119.1"
                     /translation="MNDISWFSLASTVIAAIAIAIGTIGPALAMGRAISHALDALARQ
                     PEAEKSITRTLFIGLAMIESLAIYCLVIVLIILFRNPLLSYFVTQQG"
     gene            complement(39106..39759)
                     /locus_tag="ACF2SP_000305"
     CDS             complement(39106..39759)
                     /locus_tag="ACF2SP_000305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFD2358120.1"
                     /translation="MGGLPITQSVLTTWFIMISLFIMAWSTTYKCSLLQPSTYQLLWE
                     GVLSTMYDAIKEVLPNHVELIFPFVATLWIFILVSNLIGVIPGFYSPTADLSVTASLA
                     IITFLSVHWFGIRAEGWREYLKHYIKPTPFLLPFHLISEISRTLALAVRLFGNIMSLQ
                     LTALIVLMIAGFLVPIPILILHIIEAIIQAYIFGMLALIYIAGGIQAHELKSQGESL"
     gene            complement(39801..40079)
                     /locus_tag="ACF2SP_000306"
     CDS             complement(39801..40079)
                     /locus_tag="ACF2SP_000306"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HFD2358121.1"
                     /translation="MEFKNSNKELEQQVKRNVRKINKAKKGKSTLLAQTVYLGTLGFV
                     FVLPIIAGAYLGVWLDEKIKGYSMSWTINLILLGVIIGAINVYLLIKD"
     gene            complement(40069..40479)
                     /locus_tag="ACF2SP_000307"
     CDS             complement(40069..40479)
                     /locus_tag="ACF2SP_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFD2358122.1"
                     /translation="MELFTIHLKSATQYEKIDKVISFVGEDASGQFGILAHHSRMMTC
                     LKFGLAWFRDENQETEYLALPGAVVYFIDNQLHIATRHYVRHKEYQAIQRVLAEELHW
                     EENNLLRTKESVHRLDEEMLKRLWELNRQNSYGI"
     gene            complement(40488..41912)
                     /gene="atpD"
                     /locus_tag="ACF2SP_000308"
     CDS             complement(40488..41912)
                     /gene="atpD"
                     /locus_tag="ACF2SP_000308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFD2358123.1"
                     /translation="MGHEYNWKMMDKNTAVAQSHGTPIGRIIEINGPVVRIHCDVLPP
                     LHQSLKTYTDSDEYILEVCQHLDEHHVRAITLHRASGLQRGLIVYDQGTSLQIPVSKE
                     CLGRLLNIFGEPLDGGPPLETNEYRDVLAKSEPLQMTSTQETILETGIKVIDLLCPFV
                     RGSKTGLFGGAGVGKTVLLMELMHAIIQLHQGTSVFAGVGERIREGHELWHEMKSAGV
                     MDKTLMVFGQMDESPGVRFRTGLSALTYAEYLRDTLGHEVLFLVDNIYRFVQAGSEIS
                     GLLGRMPASVGYQPTLMTEIAELEERMTSTAKGAVTSVQAVYVPADDMSDPAVTGIIT
                     HLDSIIVLSRAQAGKGIYPAVDPLASKSQFMDKILLGERHYSIAQAVREHLEHYQELE
                     DMISMMGIEELSPKDRAIVLRARKLQRYLSQPFHVTKLQTGMEGKSVSLEHTLTDCES
                     FLRGDYDEFSEEECYMRGAMDKGR"
     gene            complement(42248..42688)
                     /locus_tag="ACF2SP_000309"
     CDS             complement(42248..42688)
                     /locus_tag="ACF2SP_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2358124.1"
                     /translation="MLKRNAFILLAACSLVATPLFAEDMPSGSQMNNMGMQSSNQQST
                     QLQDEMKQMQEALKQMQEMHDKLMNAKTTEEKKSLMQTQMPMMEEGMHTMQKGMKMMQ
                     SMHDDTAKQPEMMDQRMKMMQMMTQMMQMMMDRQMMGCPMMKNQ"
     gene            complement(42793..42945)
                     /locus_tag="ACF2SP_000310"
     CDS             complement(42793..42945)
                     /locus_tag="ACF2SP_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358125.1"
                     /translation="MPKQRLIKAVIGIMSAILLLISCAPSTTTNNSHRVSHTYEGHNT
                     GGIGWH"
     gene            complement(43530..43793)
                     /locus_tag="ACF2SP_000311"
     CDS             complement(43530..43793)
                     /locus_tag="ACF2SP_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2358126.1"
                     /translation="MKKQTDLIVLGKQIRKIRKEKGFSQEGFANFIEMNRGYYGTVER
                     GEANITILNLLKILKGLDVTPNELFPPSTYVSLKRKITKNSAS"
     gene            44141..44683
                     /locus_tag="ACF2SP_000312"
     CDS             44141..44683
                     /locus_tag="ACF2SP_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HFD2358127.1"
                     /translation="MKGIVFTSLNNMIIEQFGIETWDQLVSSLDLPSGGSYTAGGTYS
                     DTELQQLIQAIAKRTNQHASVFLEAFGEYMFPILSSKYAIFLKKDMTLKEFLKSIDGT
                     IHVEVEKLYPDETLPTISYEEPAAKQLVMLYRSHRRLCHFAIGLIQGAAQHFKKRITI
                     KQTHCMLKKDDHCRLEITFE"
     gene            44649..46169
                     /locus_tag="ACF2SP_000313"
     CDS             44649..46169
                     /locus_tag="ACF2SP_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFD2358128.1"
                     /translation="MIIVVWRLPLSNENKAIFERSYYRERKAREEAELLLENKTRELH
                     LLNQDLEKKNAELLKTLKDLESVQKKLSKLAHFDVLSRLPNRLQFELDLKREIARSKR
                     YGRHLALLSIDLDFFKNVNDRFGHDVGDGLLREVAKRLCSSLREEDCVARLGGDEFAV
                     ILTEINNPQQAKLVANTMIQKMGQPFLIKGHTVMIGLSIGIAYFPDAGEEAVTLRKNA
                     DIALYNAKECGRNNCQIFTPSLRKQYSKRLGIGTELHLALERQEFFLVYQPRVDLKTN
                     SMVGMEVLLRWQHPKRGVVFPDEFISVAEHFNLIIPIGEWVLRTACRQYVDWKKSYAP
                     FNCTLAINISLHQFQHKGFISSVKHILQEYQMPSDWLELEITEATAVNFLGKIEDTLS
                     TLRNMGVKLAFDNFGSGYSFLSHLKNSPVQSIKLKQTLIEDAHLRENDNLIIKSTIAL
                     SQELGLNVVVGGVETEEQLNFLRSSHCFEVQGYYSSKPLTVGQMTQFIEERNSKGL"
     gene            46461..48254
                     /locus_tag="ACF2SP_000314"
     CDS             46461..48254
                     /locus_tag="ACF2SP_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD2358129.1"
                     /translation="MKRSEKQCCPKERILTQSQELQEGVDFSCCAQADRGTSDNFFPF
                     FTRLVQANLAKWTAGISPAAIGSSYSTWLWQLAQSPGVLWELAFYPFFHAKDCINNIV
                     CVERAADGKDVRFKKDSWQPMPWRLFAEGFLQMEDWWRRATTDVPGLPNQVERIVSFW
                     ARQSLDALSPSNFVWSNPDLFHEARRTGGLNLIQGGQIAFEDWLEKLTGAPPTGSEHF
                     IPGKQVAITPGRVVFQNHLIELIQYEAQTKTVYKEPILILPAWIMKYYILDLSPHNSL
                     VKWLVSQGHTVYIISWRNPDKEDQDLGMDDYYRQGAMAAFDAVSTLLPETKINLMGYC
                     LGGTLAMITAAAMVRDKDERLNSLTLVAAQGDFTEAGELMLFVTESQVDFLKSMMREQ
                     GYLDTKQMAGSFQMLRAYDLIWSKMVQDYMHGMRRGMIDLTAWNADATRMPYKMHSEY
                     LEKLFLKNDFAEGRYTVEGKPVAAENIQLPVFAVSTEKDHVAPWQSVYKIHLMTEGDV
                     TFVLTGGGHNAGIISEPGHSGRSYRVHEHKQGEAYLNPGSWLAMAEKREGSWWQAWHA
                     WLVQQNTKKRIASSVMNPSLPAAPGTYVLQK"
     gene            complement(48543..49286)
                     /gene="phbB"
                     /locus_tag="ACF2SP_000315"
     CDS             complement(48543..49286)
                     /gene="phbB"
                     /locus_tag="ACF2SP_000315"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HFD2358130.1"
                     /translation="MNQRVALVTGGTGGIGTAICQEMQKLDYQVIACYFKQGNHDLAK
                     EWQKKQAMQGFDIDIVYADISSFNDCEKVTELVIEKYGKIDILVNNAGVTNDATLKKM
                     TQEQWQNVIHANLNSVFNMTKTVISTMLDNSYGRIITISSVNGRKGQFGQCNYAATKS
                     ALYGFTKSLAQEVAKKGITVNTISPGYINTEMLASLRDDILEAIVAQIPVGRLGKPQE
                     IARVVAFLAEEQSGFITGANFDVNGGQYM"
     gene            complement(49376..49582)
                     /locus_tag="ACF2SP_000316"
     CDS             complement(49376..49582)
                     /locus_tag="ACF2SP_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD2358131.1"
                     /translation="MANKIRGKVKWFNKDKGFGFIESSGKDYFVHFSSIQSDGFKTLP
                     DGATVLFKMGKGQKGPQAEEVEVI"
     gene            complement(49677..50108)
                     /locus_tag="ACF2SP_000317"
     CDS             complement(49677..50108)
                     /locus_tag="ACF2SP_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358132.1"
                     /translation="MNDIKSFKIKKIVLLKITFAAIFSCTTLNAYAGLQSYHIKYHKD
                     SKINNVSLSNTEVLIEFHRSGNSLGRLLTKTNETQGITIDNTYGKNLVIHVLFIEKEG
                     KRIRCWGVSAAKTNTIEIQCDKVVSLKKTKGNTGESTDSIH"
BASE COUNT        13872 a        10247 c        11466 g        14624 t
ORIGIN      
        1 attttctttt gcacgctaaa tctttttagg tttttttgag gacacatcaa gacattttaa
       61 ttgggtttct caaaggttat tcttagctaa ctgtctgtca aagcagagaa cactttgttt
      121 tgacttatcc ataccaaaga gtctatattg taattatttc cctttaaaat caaggataga
      181 aatcatcatg aaacaaagtc attggatggt tttggcgtgt tgttttattc ttactgcatg
      241 catgagcagt ggcacgagca gtaagaatac taagagtctg gaagaacatg tcgctcaagg
      301 ccatgttggt catggtggcg gtggtggtcg ctaacactaa aatcttttac tcaatcctaa
      361 aggttaacat gagcgctcac tctattaaat aagaactatt tgggcactaa aaattgggat
      421 aaaatgatgc gcaccacatc ataatggatg gcgaatgcaa aggatggctg ttatggcaga
      481 acagtggcac gaaaaatcac ctgcagatat ggcattgttg tttgaaactg acttatcttt
      541 aggactatct gagaaagaag cacaacagcg gttaaaagaa gtgggactca acctgctgag
      601 tcaacagaaa aaaacgtctc ctttcctcat ttttttgcaa caatttgcaa gtgtggtcac
      661 ctgggtgctt ttaggtgctg ttgttgtgtc ttttttgttg gatgaaaaag ccgatgccat
      721 tgccattttg gccattgtca ttttaaatgc catcattgga tttgttttag aatatcgtgc
      781 cgatcgcgcc atgcttgctc ttcaacaaat ggccgctcct aaagccaccg tgttgcgaga
      841 tggccgcgtg agggtgatag ctgcttctga tatcgtacca ggagatattc ttctatttga
      901 aagcggtgat ttaatcgctt ccgatgctcg cctttttgaa ttatcagcgc ttaaagtcaa
      961 tgaagcacca ctcaccggag aatcgatacc agtcgctaaa aacctagaga tttgtgctaa
     1021 agaaacgcct ctagccgatc gtaaaaacat ggtgtttatg ggcacctcta tagccgatgg
     1081 cacaggtcgc gcgcttgtcg tggcgactgg tatgcaaacg gaaatggggc acattgccaa
     1141 aatgctgggt gaagcgtctc gtgatgaaac gcctttgcag aaaaaactca atcaagtggg
     1201 ttctcgtcta ttgtggcttt gctttttcat tatcattgcc atttttggct taggtctctt
     1261 acgtaacatc cctatcttca gtttgttcat gagttcagtc agtcttgcag tggctgcaat
     1321 tcctgaagga ttacctgctg tagtcacagt ggctttagcg cttggcgtgc agcgtatggt
     1381 acggcgtgcc gtactggtaa ggcgattatc ggcggttgaa acactcggct gtttacaagt
     1441 catttgcacc gataaaacag gaacgttgac ggtaggggaa atgacggcgc gtaagcttgt
     1501 gacagcaagt gatgtttata gcattcaggg agagggttat aacctgacag ggggattttc
     1561 actacaaggc caggaaatta atgtgtctga tgacacgttg ttgcaagcta ccttacgagc
     1621 aatgacagtt tgtaataacg cagaatttag cgagcaaaat ggacagacga caaccgtggg
     1681 agacccaact gaagtggctc ttttggttgc tgcagccaaa ggaggcgttt ggcaagggga
     1741 actgcgatcc tcttatcctc gcatcaaaga gttacccttt agttccgaac gcaaacgcat
     1801 gacggttgtg tgtcgacaag accatcaaaa catcgctttt gttaagggag cacccgaact
     1861 cattttggag cgctgcacct atattttgac caaaacaggc attaaaaaat taacacctaa
     1921 tgataaagcg cgcatgaaac aatcctgcga actcatggcc agcgaagcat taagactgtt
     1981 agcttttgct gagcgtcaaa tagagcctat agctttagaa aaagaagagg aagagattga
     2041 aaataacctg gttttcttag gtcttattgg tcttcaggac ccccctcatg cgagtagcaa
     2101 ggaatccatc agtcgctgta aaaaggctgg cattaaacca gtgatgatta caggagacca
     2161 tcctgataca gcaaaagcca ttgccaaaga gcttggcatt ttggaggcag gggatcgatt
     2221 gctcactggc aacgaacttg aaaacatgtc agaggaggag tttaaccatt gcgtcaaaga
     2281 cattgctgtt tatgctcgtg tgactgcgga gcataaatta aaaatcgtac gggcctggaa
     2341 aaaacaacaa atggtggtgg cgatgacagg cgatggggtg aatgatgcac ctgctttaaa
     2401 agaagcatct gtggggattg cgatgggaaa aacaggcact gcagttacca aggaagcctc
     2461 tgacatcatt gtcatggaca ataatttcac ctcgattgtg gcgggcattg aagaaggccg
     2521 gactatttac gataacattg ccaaaacact tggcttttta ttagcaggca atagtgggga
     2581 gttactggtt gtgtttgtag cccttttaat tggttggcca ttgccgttgc ttcctatcca
     2641 attattatgg attaacttag taaccgatgg tttaccggcc attggcttag ctaccgatat
     2701 gtcagagcca gggattttga atcgtccccc cagagccacg caaaaatcca tgatggatat
     2761 ggcgtttttc aaacgagtaa cctttgtggg ttgtttaacg gctttagtca ctcttggtgt
     2821 gtttgcttat gaatatttaa tcgataagga tttagtccaa gcccaggatg cggcgttttc
     2881 tgtcttagta accgccgaac ttttacgggc ctttggagca cgaagcgaca cgaagaacat
     2941 ctggcaagta ggattgtttt ccaatttaag actctttttt attgtgagca tcagtttttc
     3001 cctgcaggtc ttgattcatc atatcccggc gctgcgcgaa ttatttggca ttgaaccggt
     3061 ctcactcact cagtgcgttg tttggattct attaggcatg gtgcctttgt tagtgataga
     3121 agctcagaag cggctaagga aggcacccaa tgaagcattt tagtgttaaa aaaatgattt
     3181 ggactattct cttaggtttt tcatgtttta caggaccagt atcttatgcc aagataccgc
     3241 ctgctttatc gcagctaatc aatgaggcca cgcaaaacaa tcctcaaatt cgtgcggcac
     3301 gcgaccgact gctggcagca atccatgtga ttccacaagc gcgtgcgtta cctgatccca
     3361 agttgaatgc agggtacatc aacatgtctg agaatatccc tatggatgtg gatccaaggc
     3421 gagagcaaat gttgggtggc caacaagaaa ttccttttcc tggtaaactc attgtgcgag
     3481 gaaggatagc aacgctagag gccaaaagag cggaagcaga atatcaagcc actagtttcg
     3541 ccgttattgc tgaattaaaa cggctttatt acgaccttta ttttgtcaat aaatccattg
     3601 aaatcgtaca aagaaaccaa gagcttcttc atgaaatgga aaagagttcc gaagccaatt
     3661 acagtgtggg gaaaacaccc caacaagata tttatcgtgc ccaaactgag atttctcgtc
     3721 tgctgatgcg tttagtcatc ttaaagcaac aacggggatc cctgcaggcg ggtatcaatc
     3781 gccttttaaa tcgttcttta gaaattacaa tccatacacc agcagtgctc cctgtaacgc
     3841 caatgtgtca taatttagag tatttttata cgctcgttaa gagccgagcg ccgcaattaa
     3901 tcatgcagca acgtgctgtt caaaaaggac ggcaagccat ccgcttaagt aaaatggagt
     3961 atttcccgga tgtggaaatc gaagggggac gacttcatga tacgggaatg cacaccaaag
     4021 gctatcaagt cttgttaaaa gcaaccgtgc ctctttattt catgcaaaag caaaatcatg
     4081 ccgtgcgcga atcacttgcg cggtataatg cggatattga ggatttgcag accacctatc
     4141 gtatgctttc ttttcaagtg aaaaatgctt atttgttggc agagcgctct gcaaaactca
     4201 ttcatctgat tcaacatacc atcattccac aagccagctt aacatttact tcatcgcaag
     4261 ccacctacgg cgtgggcaaa gtggatttct taacgatgct gaataattta ttgacgctgc
     4321 aagaaaatga attggaatgg cacggtgaac tcgctgaaca tgaaaaagcg atagcacaaa
     4381 tcgaagaaag cacagggacg ttcttatgaa ttttaagatg ataatcaagg gtgtggttat
     4441 cttgagttta ctttcgttgt tgcaggtaac tcaggcgcaa caggccatgg ataatcatga
     4501 caagatggac atgaaacagt ccactcaagg aaatgagatt gtgattgatc cggctcgttt
     4561 acaagccatt ggcattacgt cagagccggt gcgtcgccaa gtggttgaaa aaataatccg
     4621 taccgtgggg cgtgttgaag ccgatgagcg gcgtgttgcc catattcatg tgcgctttga
     4681 tgggtggatt gagaacttat tcattaattt tactggagaa aaagtcaaag cagaccaagc
     4741 tcttttcacc gtttacagtc ctgaattggt cgccacccag caagaatatt tgctcgccct
     4801 taatgcccaa aaaatattaa gcaaaaatac gacatcgagt gcggctcgtg gtgcgcagtc
     4861 tgcttttcaa gcagcccacc aaaggttgtt gctgtggggg atttccgaaa aagacattga
     4921 gcaattaaga cgtacgggca aaatcactcg gacgatgacg attcattccc ccatccaagg
     4981 tacggtgctt aaaaaaatgg cgctggtggg catgcgcatt gagccaggag atgaactcta
     5041 taccattgct gatttatcac gcttgtgggt tttaggggac atttacgaat atgagcttcc
     5101 ttatattcgt cttggtcaaa tggctgattt gacccttacc tacttgccca atgaattatt
     5161 taaggccaag cttgatttta tctaccctac gattgacatg aaaacacgta cagccaaggt
     5221 tcgctttgaa gtggacaacc agaaggagca attaaaaccg ggcatgtatg tgaatctgga
     5281 actcaaagta cctttgggtg aacgtcttgt ggtccctaaa aatgctgttc tactcacagg
     5341 tgagcgcgca gtggtgttta tttaccatgg taatggcaaa attgaatggc gggacgtgac
     5401 cttaggggtt agggcagggg atttgcttga gataatcaaa ggggttaaag agggcgatca
     5461 aattattacg tctgccaatt tcctcattga ctcagagagc cagttgaaag cggccatggg
     5521 aggcatgcaa catcaataga ttttctttct gaaccgctat aatgggaaag aagctctaat
     5581 aaagaggcgt gatttggcct tggaataaag ggattttatg gtcgaacgga ttattgaatt
     5641 ttgtgcacgc aaccggttta ttgtgttgct ttttgtgctc gggttttctt tcatgggcta
     5701 cattgccttg aagaataccc gtatggacgc attgcctgac atttcagaca ctcaagtcat
     5761 tgtgtatacg acctggatgg ggcgctcgcc cgatctgatg gaagatcaag tcacttatcc
     5821 cattgtaacc gccctcttat ctgcaccaaa ggttgttgct gtgcggggat tttctgattt
     5881 tggcttttct tatgtctaca tcatttttga ggatggcacg gatatttatt gggcgcgctc
     5941 tcgggtcttg gagtacatga gtcagctggc tggaaaatta cctgaaggag tgactcctca
     6001 attggggcct gatgcgacac cggtgggttg gatttatcaa tacgccttgg tggatgacaa
     6061 tggcaagcat aacctggctg agttacgcac atttcaggat tggtatttgc gctattggtt
     6121 aagagcggtt cctggggtgt ctgaagtagc cagtgtggga ggttttgtca agcaatacca
     6181 agtcaatttg gatccagtca aagtccttgc ctacaaccta tcggttcctg agattgttga
     6241 aaaaattcgc atgagtaata atgacaccgg tggtcgagtc attgagtttt cgggcgtgga
     6301 atacatgatt cgtggtcgcg ggtacattaa gtctactgag gatattgaaa aaattgccgt
     6361 gggcaccaat gccaatggca caccgatttt attacgtgat gtggcgactg ttcagcttgg
     6421 ctcggatatg cgccgcgggg tggtcgattt aaatggccaa ggcgaagccg tgggcggtat
     6481 tgtcatcatg cgctttggtg aagacgtgtt gcaagtgatt ggccgcatca aggataaatt
     6541 gaaagaatta gcttcggcca tcccggaagg gataaaaatg gttcctgtgt atgaccgaag
     6601 taacttaatc agagaagcga tttccacggc caattggaac ttgattgaag aactggtggt
     6661 ggttgggtta ctcattattg tgtttttagg acattttcgt tccgcactca ttcctattat
     6721 taccttgccg ctggcgattt tgatttcgtt tatccccatt tatttcttac atgttggact
     6781 gaatattatg tccatcggcg gcattattgt cgccgttggt gatatggtcg atgcggccat
     6841 catcatggtc gacaatgccc ataaacgctt agcggattgg gaagcaaaag gaagcccagg
     6901 cgatcgcacc caggtgttaa tcgattcggc gaaagaggtg gggcctgcca ttttttcatc
     6961 ccttttggtc attgccatct cttttatgcc tgtttttact ttggaagctc aggaagggcg
     7021 gctgttttcg cccttggctt atactaagaa tttagccatt ttcatgagtg ctttacttgc
     7081 catcacctta attcctgtcc tattgcctct tttggtgcgt ggtcgaatca ttcctgaaca
     7141 aaaacatccg attacgcggt ggatgcaagc catgtacgcg cccgtgttga ggcttgcttt
     7201 gagatatcgc caatttgtgg tgggaattgc cattgtattg gctatggcca ccattcctct
     7261 ttataaactc attggctctg aattcatgcc ccctctttat gaagggacga ttttgtatat
     7321 gccggttact ttaccaggta tttcagtcac tcaagcgacg ggtttacttc aggaaatgga
     7381 taagaaatta aaagcatttc ctgaagtggc gcacgttttt ggcaagacgg gaagagccga
     7441 aacgtccacc gacccttctc catttaatat gatggaagtg attgtggagt taaagcctaa
     7501 agaattttgg cgcaaaggaa tgacttatga aagcctggtt gccgaaatgg atgaagcgtt
     7561 gcaatttcca ggtgtgagta acgcttggac catgcccatt aaagcacgca atgacatgct
     7621 caccacaggc atccgaacgg ctgtagggat taaagttttt ggtcctgaca ttaaaaagat
     7681 tgaagccatt ggtaaagaaa ttgaaatggt ggccaaagaa gtgaagggca ccagtacggt
     7741 ctatgcagag cgggtggctg gaggttactt ccttgatttt caaattaatc gggaccagtt
     7801 ggctcgctat ggcttaacca ttatggacgt caatcgcatc attgaatccg ctgtaggtgg
     7861 tgaaaacatt gctaccacga ttgaggggcg tgagcgctac ccagtgaatg tgcgttattt
     7921 gcgagaattg cgtgatgacc ccgacaaact caatcgtatt ttagtcaaaa cacccagtgg
     7981 tgcggaggta cccgttgcgc aattggtgac tttaaccttt cgttcaggac ctgccatggt
     8041 tcgcgatgag aatggcatgt tagcaggtta tgtgttcatt gacatcagtg gccgggacat
     8101 tggcggctat gtggaggagt taaagcaagc ggtcaacgcc aaagtgaaat tgcctccagg
     8161 ctatacgctt gcttggtcgg gacaatatga gttcatgcaa cgggtctatg agcgacttaa
     8221 aatttttgtg ccactgacct tggccatcat ttttgtctta ttttacttta ctttccgcac
     8281 cattaccgag accttgatgg tgatgcttgg cgtacccttc gctttatttg gcggcttttt
     8341 gctgctttat atattagggt acaacatgag tattgccgtt tgggtgggga tgattgcgct
     8401 tgctggtgtt gcggctgaaa ccagtgccgt gatgctcgct taccttgatt ctgattacaa
     8461 tgaacaaaag gaaaaagggt tgctcaaaac attgcccgat ttaattcagc tggtacaact
     8521 ttgtgcggtc tcccgcattc gtcccatggc catggcgggt cttgctaaca tcattggcct
     8581 attaccggtg atgtgggcta caggcattgg ggctgatgtg atgaagcgtc ttgcagcacc
     8641 catggtcggt ggggtttttt ctgccctttt attgacttta attgtgattc ctgtggttta
     8701 tgtgatgtgg cgttggcaag tggatttgaa aaaaacagca gacttaacgg aggcaagatg
     8761 aaattaactt ttttaggagc aactgagacg gttactggtt ctaaatatct tttgaccatt
     8821 ggttctaaaa aaatcctgat tgattgcggt ctttttcaag gctacaaaga attacgatta
     8881 cgtaactggg cgcccttgcc aattgatccc catgatattg atgcggtgat tatcactcat
     8941 gcgcatattg accattcagg ctatcttccc ttgctcgtca aaaacggatt ccaaggcaaa
     9001 atttatacaa caccgggtac caaagcactg tgttccattt tgttacccga cagtggtcat
     9061 ctgcaggaag aagaagcacg cttggccaat aagtatggat tctccaaaca ccaacctgct
     9121 cttcctcttt atactgaaaa agaagctcgg acagcattgc agtattttga aaccattgat
     9181 tttgataccc cgcatcaatt attccatgat ttcagttttg agtatcatcg tgccggtcac
     9241 attgtgggtg cggccatggt caaaataaaa acccaaaagg gttccattgt gtttactgga
     9301 gacattggcc gtccttatga tcccgttatg aaagcccctg ccttcattca agaaacggat
     9361 tatctggtga tggagtccac ctatggtgac cgattgcatg atgccacaga tcccttaccc
     9421 caaatggctc aggtgattaa tcaaaccgtt aaacgagggg gctcagtcat tattcctgcg
     9481 tttgcagtag ggcgtgcgca aagtcttctt tattttattt atgaactgaa acgacaaggg
     9541 caaattccca aagaccttcc tgtcttttta gacagcccca tggccattga tgccactcat
     9601 ttactctgcg cttataaaga agaccaccat ttaactgcag aacaatgtcg tggactatgc
     9661 catgttgcca cctatgtgaa cacacctgag gagtccaagg agattgaccg tcatcacatg
     9721 ccgcaaatca tcatagcggc cagcggcatg gcgcaaggcg gccgcattct gcatcattta
     9781 aaagcctttg cgcctaatcc taaaaatact ctacttttta ctggctttca ggcaggtggc
     9841 actcgtggtg ctcgaattgt gaatggggag aagcaagtca aaattcacgg gagttttatt
     9901 ccgatacaag ctaaggtcgt agtcatgagc agtacatcgg ctcatgccga ctacctggaa
     9961 ttgttagaat ggctaactca tttgactaat ccgcctaaaa aggtttttat cacccatggg
    10021 gagcctcaca gcgcccaatc gctaaaacag aagattgaga agcaattagg tttttcttgc
    10081 accattcctt cttatctgca aacggaggaa ttgtggtgag taagaaaacc tctcatgggc
    10141 ttcggttaaa acatttaggg attaaaactt atcacgaagc tattatttac atgcgcgagg
    10201 attgtcatgt ttgtcattcg gaaggattcg aagtacagac gcgtatccaa gtgactttag
    10261 gtcagcactc tatgatagcc actcttaatg tggtgacctc agagctactt cagcctggtg
    10321 aggctggttt gtcggattac gcctgggagt ctttacatgc caaagaaggc gatgaaattc
    10381 aggtctccca tccaaaaccc ttggaatcct taagctatgt gcataccaag atttatggca
    10441 aagaactgtc ttatgaacaa atgaagatca tcattgatga tgtgttaagc ggtcgtcttt
    10501 ctgatgtaca aatctccgct tttttagcgg caagtagtgc aggacgtcta acccgtacgg
    10561 aaatcatgaa actcaccaaa gccatgatag acagtggtga tcgcttatcc tggtcttcac
    10621 ctctggtcgt cgataaacat tgcgtgggag gattgccggg aaatcgcaca actctgattg
    10681 tagttcccat tgtggccgct tttggattga tgattccaaa aacatcatcg cgtgccatta
    10741 catcacccgc ggggactgcg gataccatgg agactttagc tccagtccac ttaagtcccc
    10801 aaaagatgcg ccaagtggtt gaacaagaaa acggctgtat cgtatggggt ggggctgtga
    10861 gtttaagtcc tgctgatgac gtgctcatcc gtgtggaacg agccattgat ttagatagtg
    10921 aagggcaatt agtggcttcc attctttcta aaaaaattgc aacaggggcc acacacgcgg
    10981 ttattgatat ccccgtagga cctactgcca aagtgaggaa tcagtctatg gcgctccttc
    11041 tgaaacaatt gcttgaggag gtaggtaacg agttagggct tgttgtccat actttattta
    11101 cggatgggac gcagccggta ggccatggca ttggcccgtc gttagaagct cgcgatgtat
    11161 tggcagtatt acaagggttg cctgatgcgc ccaatgattt gcgtgaacga gcgttgacgc
    11221 ttgcaggtgc tgcgttagag tgttcttcga aagtaccaac gggtttaggt aaatccattg
    11281 cgacgcaatt attggatagt ggacaagcgt ttaagaaatt tcaggccatt tgtgaggctc
    11341 agggtgggat gagggaattg accaaggcac gttttactta tcctgttgtg gctgcaaagg
    11401 caggaaaagt ctccctcatt gacaatcgaa agcttgcaaa aatagctaag cttgctggtg
    11461 cgcctaaatc gaaatcggcg ggtattgacc tccattccca tgtcggcgaa tccgttgaaa
    11521 aaggcgaacc tttgtttaca attcattcgg agtcttcagg ggagctccat tacgcctgtg
    11581 atttacttcg cgataaacaa gacatcatta ttttaggaga aaattcttga agcctctgtt
    11641 atgttcatta tttgattctt cagaaattgc cggacaatta caaaagacac ttcaagtgga
    11701 agtgggtaag gtcacattcc accgttttcc tgatacagaa tggtacttaa aaattgattc
    11761 agaggttaaa agtcgcagca ttatggtagt ggacagttta aaccagccgg atgaaaaaat
    11821 actgcctttg ttgttttttg ccaaaacggc taaagagttg ggggctaaaa aaatcggttt
    11881 gattgctcct tatctttcct acctacggca agataaacgt tttcacgaag gagaaggaat
    11941 tacttcacgt tattttgcgg acattttatc gacttcattc gattggctca tgacgattga
    12001 tccgcacttg catcgctatc actctttaga tgaaatctac tcaattccca cttttgtttt
    12061 gcatgccaca tcgatgattg ccacatggat taaacatcat gttcctaagc caattcttat
    12121 tggtcctgat atggaaagtg agcagtgggt tgccgatatt gcagaaaaag gtcgttttcc
    12181 ttatgcgatt ttggaaaaga ttcggcatgg tgataaagag gtgactattt cagtacccta
    12241 tattcctgag ctcgaatctt cgacactcgt gctcgtcgat gacatcattt ccaccgccag
    12301 aaccatggta gagacggtga agcatttaca acaagcaggg agcaagtcca tcgtttgtat
    12361 tggtgtgcat gcgctgtttg cagaagacgc ttattctcta ttatctgcga tgaaaggggt
    12421 gcaagtcatc acgtgtaata ccattcatca tgtcaccaat gcgatcgatg tgagtggttt
    12481 ggctgtggac gttttagtcc aaaacaaact ggccgcttta aaaagtaagc gtaaatgagt
    12541 tgattattaa caaggagtat ccatgttgaa agcctttaga atgattgcag tgcttattgg
    12601 gttaatcgga ggaccattct ttttgagttc ctgcgatgga gcagtgaagc atggctccca
    12661 agaggtcgat ggcggctatg gaggacacag cgcgggtggg catggtggtc atggcggtgc
    12721 tgggcattaa gcccattcgc tgcattggta aatcaagcca aagcatactc acacttaaca
    12781 ggtagggcaa accatgaaac acgaccacca tcaaggacat acccattctg gtaaaggaca
    12841 tgcttgtcct cacgaacata attctcccaa gactcagcaa gcctcttcca aaatggaggg
    12901 gcctattgtt tatacgtgtc ccatgcaccc agaaattcgc caatctgccc ctggtcattg
    12961 tcctctttgt ggtatggctt tagaaccgga aacggtgacg gtttcggagg ttgtgagccc
    13021 tgaatatctt gatatgagac gtcgattttg gattgcattg atgttaacta tacctgttgt
    13081 gatattggag atgggtggac atggtttgaa gcattttatt tctggtaatg gttccagttg
    13141 gatccaattg cttcttgcca cgccagtggt gttatgggga ggttggcctt ttttcaagcg
    13201 cggttggcag tctctaaaaa caggccaact caatatgttt accttgattg ccatgggtat
    13261 tggtgtggcg tggatttaca gtatggtggc tgtcctttgg ccaggtgtat ttccccatgc
    13321 gtttcgaagt caagaaggag tagttgctgt ttattttgaa gcggcagcag tgattactac
    13381 gcttgtcttg ttggggcaag tgctggaact aaaagctcga gaacaaacgg gtagtgccat
    13441 ccgtgccttg ttaaaattgg tccctgaaag tgcacaccga attaaggaag atggcagtga
    13501 agaggaagtg tccctggata acgttgccgt tggtgatttg ttgcgtgttc ggcctggtga
    13561 aaaaatacca gtggatggtg aagtgcaaga aggccgaagc tttgtcgatg aatccatggt
    13621 gacgggtgag cccattccag ttgctaaaga ggccagcgct aaagttattg gtgctacgat
    13681 taatcagacg ggtagctttg tcatgaaagc gctgcatgtg ggcagtgata ccatgcttgc
    13741 acgcattgtg cagatggtga gtgatgccca acgaagccgt gcgccgattc agcgactagc
    13801 cgataccgtc tcaggatggt ttgtgcccgc tgtgattttg gttgcggtgc tgtcttttat
    13861 tgtgtgggca ctgttgggtc cgcaaccagc gcttagctat ggtttgataa ctgctgtgtc
    13921 agtactcatc atcgcttgcc cttgtgcgtt aggtttagca acgcccatgt ccattatggt
    13981 cggtgttgga aaaggagcac aaagcggggt attgattaaa aatgccgaag cgttggaacg
    14041 aatggagaag gtcaataccc tggtggtcga taaaacgggc acgcttactg aaggtcaccc
    14101 taaattgaca cgcattgtga ccgatgattt cgtggaggat aatgctttag cattagctgc
    14161 ggcacttgag catcaaagtg aacatccgct ggccaatgct attgtgcatg ctgcaaaaga
    14221 aaaaggattg tcccttggaa cagtcgaagc gtttgaagcg cccactggaa aaggggttgt
    14281 aggtcaggta gacggccatc atgttgctat tggtaacgcc agattaatgc aagagcatgg
    14341 aagcgataat gcccctcttt ttgaaaaggc tgatgaactt cgtggtaaag gcgcctcagt
    14401 catgtttatg gcggttgatg gtaaaacagt agctctcttg gtggttgaag accctattaa
    14461 atcgagtact ccagagacca tcctcgaact tcagcaaagt ggcattgaaa ttgtcatgct
    14521 caccggagat agcaaaaaaa cagcagaagc ggtggcaggg accctcggca ttaaaaaggt
    14581 ggtagctgaa atcatgcctg aagacaagag tcgcatcgtg agtgaattaa aagacaaagg
    14641 tcttattgtt gccatggcag gagatggggt gaatgatgcc ccagcgcttg ccaaagctga
    14701 tattggtatt gccatgggaa caggcactga tgtggccatc gaaagtgctg gggtcacctt
    14761 gttgcatgga gatttgcgtg gtatcgccaa agcgcgacgt ttgtcagaaa ataccatgag
    14821 caacattcgc caaaatcttt tcttcgcatt tatttataat gtactgggtg tgcctttggc
    14881 tgctggggtt ttgtaccccc taaccggctt gctcttaagt cctatgattg cagcagcggc
    14941 gatggcgctt tcttcagtgt cagtgattat caatgcgctg agactaaaga gagtgacact
    15001 atgaaaaaag gatggtcaga tgagcttaag atgtgattag tctttattgt attggctaaa
    15061 caaaaatcat cacgatcact tcattgaaga aaaatcaagt ctatactaaa aaaatagttc
    15121 agactgtatt taaggcaaaa tcatggttat cccagttgtt atgatagtgt ttggtattgt
    15181 ctttcttgct aaaggagctt atttaatcaa tggattagtg caacaaggga atcactatcg
    15241 gcgactaagg tttgtttctc tttcttttat tggcattttt atcctcagtt atatagccag
    15301 cattttttat ggtttattgg tcgatcctca ttgccttaat tatttttatg gcagtgtatt
    15361 tttgttagca gcgacttata cttatttttc gtgtcatttg gtgactaaga ccattcaaaa
    15421 agcggaatta atggctgatt ttaaaagtcg ctacgaacta ttaaggcagc atgctcagta
    15481 tgattcgcta actggctgtc ataacagaga gacccttttt gaggttttga acacaaggct
    15541 ccaacaaatt aagatgcatg gaggatgggt tatcgttctt tttattgata tggataaatt
    15601 taagacggtc aatgaccgtt atggtcatga tgcgggagat aacacgttac aaacatttgg
    15661 gcaactccta acgcagcgat tgcgaaagga tgatatcgtg gctcgttatg gtggggatga
    15721 gtttattgcc gtgatggaaa acatctctat cgatgaggcg aaaaagattg ctgaaaatct
    15781 tatcactgtg acgagacaat cgttcaaaga aggtatttta tcggagctta atctaggttg
    15841 ctctattggc atcactcaga tgaatcctca ctcggcctca gtgaataccg tcatcaaaga
    15901 agcggatttg gcgtgctacg cggcaaaaca aagaaaaatc aatggctcgg tttgtgtcta
    15961 tagtccaatg cttgcaacaa caaaccatct catcgacacg agtatccatc caatcgctga
    16021 ctagaaaaag agaatgcata aaacagtcat cttactgcag aagcaggtac gacatcatct
    16081 attattttac aacccattgt ttaatggcat tgggtgggag atactagaaa ataaaaataa
    16141 taaaaagaag ggattaattt atgactcatt atctgatgaa acgcaccctc ctggtgataa
    16201 ttcccgcctt tctggtgggt tgttcctata atgccaagtt aacccctgaa ggagaaaaag
    16261 taaccattct ccagtcaggt cagctctcaa aaggctgtac ttttttaggg gaagtcagag
    16321 cctacgatcg caatggcatg agccaatcct atcaatcaca cgagcatttg atcaaggacg
    16381 aacgaaatat cctaagaaat gaagcggcgt tattaggtgc taatacggtt ttggtgagtc
    16441 aacatcagca aacttatgaa ggcaatccca aaaatgattt cgtggcagac cattggatgg
    16501 taggtcgagc ctaccgatgc aagataaaac aatagataga cctctttgag ccattgttta
    16561 tggaatatca tcgtgcttgg cggttttctt atagggtcgt tcttctgttg ctgtatgcct
    16621 ctttggcaac agcggggaag gcatggactc ttcaggaaat aactcaaatg gcttttgcaa
    16681 acaacaagga tttaaaagcc gctcgttata atgtggctat tgccaaggcc cgtttgctac
    16741 aagctggcct ttggcccaat ccaagcctca acctctccaa caacgacgat cgcttattta
    16801 atgatgaagg ggaatattca cgaagtgctg ggtttaatca agcattccct atttctgggc
    16861 gtattgcaaa gcaaaagaca gtggcgcgtt tagatgtatt gaaagccatg gcagaaatca
    16921 gggaagcaga gcgccaatta agcgccaaag tagcaaatgc attttatgcc gcgatgatta
    16981 ctgagcgtcg cacgcaacag gtgaattact tattacggat aaaccaagag cttgttgatg
    17041 tgattcataa ccgttatcac gcggctgaaa tttcaaagct ggatgagaat gcggcacgca
    17101 ttgagtatct gcgtatcggg caagaaaagc acctgttgca tagtcttcgt attagccaat
    17161 atgcgctact gaatcaattg atggggcgag aagccaattc acccttatcc cttaagatgg
    17221 attttatcgc tgagagaacg ctccttaaat taccccgctt aaaagcctta gcgcttaaaa
    17281 atcgtccaga ccgtcaatcc atcgcattat cgattgaacg cgccaatgcc gatcgccgtt
    17341 tggccaaagc cgagcgtttt gctgattgga cggtagggct tggggtacaa caagataaaa
    17401 ttgtggtcga agagggccca cctcaacctg ccgataggac tttgggtgtg actctttccg
    17461 tgcctcttcc tctgtttaat cgtaatcagg ggcgaatttt agaagcaagt cacacaggga
    17521 ctcaggcaat aatggctttg cgtgctttga atttaagtat tgagacggaa gtaaccagta
    17581 actatgggca attaaaagcg ctgcaaatga gtcttaggga aacgcaacag gtttctctga
    17641 aattagcggt tgagaatgtc acactggctc gtgactctta tgagaatgga caaatttctt
    17701 ttttgaatgt gctgcaagta caaaaacaac aaaatgattt acaaacgact tatctcaaca
    17761 cgttagaaaa gtatttgcaa gtgtatgtgg ctttatgtac agctattggt cctggtaaaa
    17821 ccaatgggtt ttgcccttat ttagcgtatc aaaggaatgg ggatagtaaa tgagatggca
    17881 acagcgcatg agaaggatgg cattaattag tctgtttctt agtctactct ttcctattca
    17941 agggtgcttt gcccatggag aagaaattga agtgagtgag ggaggagcta aaggcccagt
    18001 tcatttaaca ccccagcaaa ccaagatgct tgacatcaag gtagtggaag caaccaatcg
    18061 tcctatggcg caaatgcttg gtttaaatgg ccaaattcaa ttgttacctg atgcgcaagc
    18121 ggatgttagc attcggatta gcggaagtgt gacggccatt gatgtgaatt taggagataa
    18181 tgtcaaggca ggacaacgct tagccacagt gcaatcaaga ttagtgggta atcctccacc
    18241 aagtgttgcg gtcacggcac ccattgcggg catcattgat gccaggaaca ttaacttagg
    18301 tcaagccgtt gaaccgaaca ccgttctttt tcacatcagc aatcgcgaca aactgttagt
    18361 catcgctcaa gtgtatgaag aagatttaga taagattaag gtagggcaag aagtgaatgt
    18421 tcatgcccta agttacccca agcgaacttt tccaggccaa gtgactttaa ttgaacccaa
    18481 tcttgactcc ttaacccgta ccgtgaatgt gcgcatcacc ttggataatg aagagaaact
    18541 cctaaagccc ggaatgtttg tacgcgccaa cgtcatttta gcgcgtaatg aagcagcact
    18601 tgccgtaccc aatgcggccg tgttgcaggc agataacgag tcgtttgttt ttgtgcaaaa
    18661 taaggatatt tatgatcgcg tcgttgttca aatgggtgcc gtggatgatg agtattcaga
    18721 aattaaagac gggctagttc ctggcgacct tgttgtgacg caaggacatc gtgagcttta
    18781 tactttgtgg ttgagtgggg gtagtaagcc tcattcaggc caagaggagc ctcactaaat
    18841 gtttacgcac ttaattgcct ggtcgttaca taatcgcccc ttgattttgg ccttaacgat
    18901 tattctttgt ttgctgggtg gttatacttt aaaacaaatg ccagtagatg tgtttcccga
    18961 atttgcccca ccgcaagtgg tggtgcaaac gcaagcgcct ggcatggcca cgcaagatgt
    19021 agaaacttta attacttatc ctttggaaag tgcgattaat ggcacgccag gggtggttag
    19081 tgtgcgctct aaaacgtctg tgggactgtc caccatcacg gtggtgtttg acgaccaaac
    19141 tgacatttat cgtaatcgcc agttagttaa tgaacgcatt caacaagtag tcaatcgcct
    19201 ccctcctggc gttgagtcac ccatcatgtt accggttacg tctgccgtgg ggtggttggt
    19261 caaatatgcg ttggtgagcc gcaccgaaag tcctgaaacc ttgcgcacta tttcggattg
    19321 gacgattcgt cccaggattc tagcattagg tggcgtagct tctgtagttt ccctgggtgg
    19381 tgaggtcaag caataccagg tgcgcctcat tcctgagcgt atgctcgctt atcgaatctc
    19441 ggtggaagac gtgcgacaag cgctgacatc ggcgaatcaa aacgtacccg gtgcctttgt
    19501 tcaccaggca ggaaccgaat tcgtagtgag tacccttggg cggattaaga cgcttaaaga
    19561 catcaaaaaa acattgattg tggttcgtaa tggggtacct attaccatga ataatgtggc
    19621 aaatgttgct tttggtggag aaattaagcg aggtgatgga gcttataata cccaaaatgc
    19681 ggtgattggg acaatttcta aagcctatgg ggctgatacg gtcacgacca cagagaaagt
    19741 cgaaaaagca cttgcggaaa ttaaaaagtc cttgcctcac gatgtgacct taatgactaa
    19801 tgtgtttcgc caaaccagct ttattgaatc tgccattcac aatttaactc gggctttgct
    19861 tgaaggggct gtgattgtta ttgcggttct ttttgttttt ttgatgaatt ggcgcgcttc
    19921 gtttattacg ttcctttcga tgcccgtttc gttcgtagtg ggtattctag tgcttcatta
    19981 ttttggtatt ggcatcaatt ccatgacctt aggtggtatg gccattgcca ttggagaagt
    20041 agtcgatgac ggcatcatta cggtggaaaa cgtggtgcac cgtttacgga ttaatcgtca
    20101 ggaagcccat cctttaccca ccatacaagt agtttttgat gcggtgcttg aaattcgaag
    20161 ttcagtggtt tatgccacga ttatcattag tttggttttt ttacccatct tctttttatc
    20221 ggggattgct gagcatattt ttagcccctt agccattgcc tacattgcct cggtattggg
    20281 ctctctcgtg gtctccatca ctatggttcc tgctttgtgt tactggttac tggtgtgtgg
    20341 tcaagaaaaa cagcaagatg ttgaagtgag tctgcacgct ttatcgaata aggaacggca
    20401 ttatgccgta gaaaaggaag gtaaccatca ggcggagtcc gaaactcgct ttgtcttgtg
    20461 gcttaaaaag cattttttaa cagcactcca atggtctatt gcccactgca aaatggttct
    20521 tgcgtttgca ttatcggctt ttgtctttgc actggcgtta cttccttttt ttggcacgtc
    20581 ttttttaccg gagtttcatg aaggcaactt tattgtcgtg atgagtacct tgccgggaac
    20641 ctcccttaat gaatccatgc gtttaggtca acaagtacaa aaagcattgc ttcgctatcc
    20701 gcaagtgatt tccattgcgc agcgggcagg gcgcagtgag cttgatgaag atgcgctacc
    20761 acccaacatc agtgaatttg atgtgcttct taactttgat aaagacaagt ccatgagacc
    20821 tgatgaattg ctgcgtcgca ttcgtgcgga tttggcgaat attcctggag ccgtatttaa
    20881 cgtggggcaa ttcattgctc atcgtatgga tgaagtgctt tctggggtac gcgctcaagt
    20941 ggccgttaag atttttgggg ataatttatc cacactcaat gaactaggac aatcgatgga
    21001 gaccctgtta aaatcagtac caggggtggt ggatgtgaat aaggaacaac agattaaagt
    21061 ccctcaattg gtgattcaac tcgatcgcga aaaagcagcg cgctatggag tcaatgtggg
    21121 gcagatttcg gaagacgtgc aagtgcttct gaatggcgtg agtgtttcca gcgttttaga
    21181 ggggcaacgc acgtttgatt tgtatcttcg aatggataaa ccagggcgtg acagcgtcaa
    21241 gaacattcaa aacatgctga ttgatgctca tggcgtgggg caaaatacca ttacgcaaat
    21301 cccattgcgg gctgtggctg agattgcatt ggaaccgcaa ccttttgcaa ttaatcgtga
    21361 gaacgtgcaa cggttgctgg tgatttcttt taatgtggag gggcgggatt taggtagcgt
    21421 gattgctgaa gtgcaacaag aagtgcagga aaaaatcaaa ctacccactg gttattttat
    21481 tcaatacggc ggccaatttg aaagccagca acaagcctcc agagtgattt tggtttttgg
    21541 tggcttggtg atttttgtga tgctgatttt gctgcataaa gcgtttggga cgtttcgaga
    21601 ggctttattg gtgatgttta atttgccctt ggcacttatt ggtggggtca tttcattgtt
    21661 tgtggtgagt ggcgagatga gtgtggccgc catgattggg tttattacgc tgtttggaat
    21721 tgcggcgaga aatggcatta ttttggtgag ccactataat caattacgat tgcagggaaa
    21781 aactcgggag caggtggtca ttgatggcac catggaccgc ttggtgcccg tattaatgac
    21841 agctgctaca gcggcactgg gcttgattcc tttgttgtgg ggttcgcctg ccggaaagga
    21901 acttgaacga cctttggccc aagtgctttt aggtgggtta ttgacttcta cggttctcaa
    21961 tatgttggta gtacctacgg tgtataacgc cattgaagta tggcgaaaaa aacggatgca
    22021 attcaatgaa acagaacaag gagaaataac atgaagcaca ttactttgaa acaaaaaaac
    22081 cacttctggt tggggcttat agccagtgtt ttgatggttt tcgggagctc ccaggttttt
    22141 gctaatccaa ctgaatctat tcctaaaacc gtcccagcca tttgggaagc aatagacaag
    22201 cattctgcat ccattaatca aatattaggc ggggatgatt taacgtccat acacgagcat
    22261 gcgtttgcca ttcgtgattt agccaatgca ttacctgctt taagcaagga tttatcagag
    22321 gagcagaaaa agacactgca acaaaatcta agctatgttg gtcaattggc gactcgtctt
    22381 gataaaacag gagatgccaa tgataaagaa gggacgcaag ccaactggca aaaattgcaa
    22441 aaagtgttag cacagctgcg tgcgctgtat actcccaatg cccctaatta atcaaggaat
    22501 gagtcatgag gggtgcagga attcaatgga ttgaacattt ttgtaaaaat ctgtccacaa
    22561 cagataaaga tgtggattat tcaggatggt ggtgacgcat ggacaaggaa aacaccgatt
    22621 cgccttatca aatggggcat gattttattt cttatcaact ggattgttgg cagcgttcta
    22681 ttttattttg ggataccttg cgtgaacgcg ccaataatat gatggagcac gaacaacaag
    22741 ggttgccacc actgcttaat ttcaaatatg aattagtttt ggatgggaaa tcattggaac
    22801 ccaaaaccaa ttatgcattg gttaaaattc ttgaggtcgg tgatgtgtgc tttgaagagt
    22861 gttttgatcc tcatgctcat ccggtgatta ttgtggatcc cagatcaggc catgggccag
    22921 ggattggtgg tttcaaacgg gactctgaag tgggcgtagc acttcatagt ggtcatcctg
    22981 tgtattttgt tattttttat cctaatcctg tgcctcatca aacgctcgcc gatgtcctga
    23041 tgactttgcg ccattttgtt gaaaaagtcc aagcctggca tgaaggtaaa gcccccattc
    23101 tttatggcaa ttgccaagcc ggttggatgc tggctttatt ggcctctgat tgcgcgagat
    23161 cggttggctt gacagtcatg aatggctcac cggtttctta ttggtccaac agcgaagaag
    23221 aagccaatcc catgcagtta ttagggggac tcttgggtgg ctcctggagt gcccgttttc
    23281 tatcggattt aaaagaaggg atctttgatg gggcgtggtt ggtctctaat tttgagttac
    23341 tcaatcccac cacagccatt tgggataagt attatggctt atttgatgaa attgacacag
    23401 agcaggagcg attcttggag tttgaacgct ggtggaatgg gttttaccaa tttagccagg
    23461 aagagataat ggccaccgtg aataatcttt ttattggcaa tcaactagag cgcggtgaaa
    23521 tgcggattca taaaggatgt gtttacgatt tgaaacgcat tcaaagccct attgttctct
    23581 ttgcttccca aggcgatcaa attaccccac cgaggcaggc tttacattgg ataagaacaa
    23641 tttatcctac gacacaagca ttgaagaagg ccaaacagcg agtggtgtat ctgcttcatc
    23701 cttccgttgg gcatttagga atctttgtat cggctaaggt cgttcgtttg caccatcgtg
    23761 ctattttaga gcatagtgat gccattgagc aattgcagcc agggctttat gaaatgataa
    23821 tcgttaaccc aacaggcgat cccgattgca gtaaagaaca atatcatgtg cgttttgaag
    23881 cgcgcgaact cacagaactt tgcacaacga gctccacgca acagtttgac aaggttcgtc
    23941 aaacatccga agccaatgac tcgatttatc aaaaagttat ccaaccttcc gtgcagagct
    24001 taagtaatcc ttttttatcc tggtgccttg agaaaacgca tcccatgcgc ctaagtcgct
    24061 atgtgttttc tgaaaaagtg aatccgctga tgaaagcaat cgaactatta agcccaccca
    24121 ttcaagccaa tcgccgcatg gctacacaga gcaattgttt taaaaaaata gagcatggtg
    24181 ttgcgcagat gatgagggat tcgttggaat cctcccgaat catgcgtaat gacgtgatgg
    24241 caaattggtt tgaagccctt tataaggatc ccgattaaca ggcgaggaaa ttaattcatg
    24301 gagcgtatgg catcaaattc ctcgtcaagg ttcatttcac tctctaagtc cgcttcatga
    24361 acccctttct catcaaaaag agggttagtt tctcgagctt tttcttcgta gtcattctct
    24421 acaaaacaat ccttagcgtt cataacaatt ccttcaaaat gattgttctt gtccttgcca
    24481 acgactaaag acaacccggt ttattgttgc tattttcatc tctgacattt agcataacgg
    24541 atacgattca atgaacaaaa ataagaaata aaaaggggtc aactgaatag ccatttggct
    24601 atttttttat aagaagaaac gagggaatgg gatgttggtt ccccaaactg gaataaggaa
    24661 cgcatcgacc atgatagaaa ttatccccaa ttggcacccc atttttgtcc attttaccgt
    24721 ggccttattt actgtttcgg tgattcttta tgccctaacc tatgtatcct cttacattca
    24781 ttggaacacc aagccattga ttgtcgaatt ggaaattgtg gcgcgttggt gtttatggct
    24841 ggccgcattg agcacgatga ccaccgtgtc cgctgggttt tatgcctttt atacggtaaa
    24901 acatggtgct atggcgcacg ccgtaaaagt gattcaccgc aactgggcac tggctactgc
    24961 aagtgtcatt ttacttatgg cgttttgggt tgtttggcgt tatattaaac accaaaaacc
    25021 gacgctgctc ttcttaatgg ctttattcct tgtgcaagtg ctgttgctca caaccgcttg
    25081 gtatggggcg gaattagtct atcggcatgg gtatggtgtt ttgcctgtca cagcagaaaa
    25141 gacggtatca ccgcattaat acgattttta tggaaggatg gaaacatgtt acaaaaggat
    25201 ttgcgttgtt caattaggaa ttattgcttt attttttcct ttttactgct cggttcttct
    25261 gctgtgtttg cccagcatga gcaacatggt gcctcaacac aaccgttaac ttcctcgaag
    25321 agtacaccat ctcaatcgaa acctgcacaa tacaaaatgg aacaacctac cactaaaaaa
    25381 caatctccta aagcggtgac gcctctaact attacaggtg gagcaaagcg tacggttaat
    25441 ttggtggttg cttataaaac ggtgaatttt gcaggcaaac caagacgcgc cattgctgtt
    25501 aatgggcaaa ttccagcacc gacgttgcat tttaaagaag gggacgatgt caccatcaat
    25561 gtgtataacc atttggatga gggaacgtcc attcattggc atggtctttt agtcccttgg
    25621 caaatggatg gggtggacgg ggtgagtcaa aaaccaattc ccccaggagg cgtgttccat
    25681 tatcgcttta agctttacca acgagggacc tattggtacc atgcccatgc caaggttcaa
    25741 gaacaagaag gcttatacgg gacttttatt attgacccgc ctaatcctcc aagctatcac
    25801 tacaccaaag attatgtggt ggtgttatcc gattggagca acacccctgc ggaacaagta
    25861 cttgcgaatt taaaaaaaga cggcgactat tacggtcctc gatttcccct acaaccctcg
    25921 cttatgaagt ttcttcatga ttatcgtaaa gcgtcatccg aagagcgtaa aaaattaatt
    25981 gctgattata aaatgatgca acaaatgcgc atgagtatct atgacttaag tgacgtggcg
    26041 tatgatgcgt atttattaaa tggtcaccct aaatctcatc cttggaccgc ccctgtgaaa
    26101 gtaggggata gggtgcggct acgcttcatt ggcgcaggag caagcaccat ctatcgtgtt
    26161 aaaattcctg atgccaaagt ggagatggtg catattcagg gaaatgatgt aaccccctat
    26221 cccattgagg atttttggat tgcaccaggt gaaacctatg atatcctggt gaccattcaa
    26281 aaaaacgagc cttacattat ttatgccgag tcgattgaca cgctgggcaa agcgtatggg
    26341 gctttagtga ctcatcctaa tcaagtcgtc aactatcagc acgtgactcc tttccctgag
    26401 ccacttcctg tcacaagaga aatgatggcc aacatgatgc agtctatgaa tagcgggggg
    26461 atgagtggaa atcaacaaca cgccttgatg aacaaaaaaa catcatcaac ggcgatgaaa
    26521 ccctccatga ccatgccttc gcactctcaa gcaatgagtt cgcaaagtgc taatcattcg
    26581 atgtcctcta agactatcca aaagaaaatg gacaatgcat ccaatcattc cgctcacacc
    26641 tcatcaatga agtccaaatc ctcatcgacc tcgatgaaca agagtatgga catgccaggt
    26701 atgaatcatg gggcaatgag tcagagtaag acgcctaact ctgctcaaat gaaatctgac
    26761 ccctcgtcga ccttaatgaa caagagcatg gccatgccgg gcatgaatca tagcgcaatg
    26821 ggtcagagta agaagtctca ctctgctcaa atgaagtctg actcctcgtc gaccgcaatg
    26881 aacaacagca tggctatgcc ggacatgaat catagcgcaa tgagccagag taagacatct
    26941 cactctgcta atgacatgtc tatgaatagg aacatgaagt cagataggcc tatggaacat
    27001 ggcatgtcga tgaatgattc catgaacatg cagatgccta ttgagccgac catcataggc
    27061 gataaaatag agccgcctga ttcagcaaaa gccaccacct taggaaccaa ataccaagag
    27121 ttaaaagcag cagtgaaaac caataatccg aataaaccag tcgatgggat tattaaaatg
    27181 gaattgtttg gttatatgga ccgttatatc tggtttatta atggcctacc agaatacaag
    27241 gccaaaccga ttttgattga gccaggcaag cgttatcgga ttatatttac caataattcg
    27301 atgatgcgtc accccatgca tattcatggt cattggttta ttttacgcaa tggtcatggg
    27361 gcttacgatc ccttattaca taccattgag gtggctccag gagctactgc cgttgccgat
    27421 tttgatactg aagccagcgg ccaatggttt ttccattgcc accatcttct gcacatgacc
    27481 gcaggcatgg cgcgggtatt tcaatacacc accatcattg agattgccaa aggcactcga
    27541 aagccagaca attacgccta tcagcaagcc tacatcaatc ggccggtcgt acgagaagac
    27601 gaagtcatgc cactggatgc ttctctcatc aaacaccccg cagggcatca tcaaggtttc
    27661 tatcgttcaa gctatattga actcggtgaa gatccttttc ataatgctca ggaaatgacg
    27721 tttagaggcc tctatggccc agattacaat aagctccagt tgtatactga ggacgctgaa
    27781 atttataaag gaaccgttga aaacgcagat atcgatgtgt tctattggca tttgattagc
    27841 caattttggg cagttaaagg aggagtcaat tatttttatc gccccggagg cccttattgg
    27901 caacctggta ttggtattga agggttaatg ccttggtata ttgataccaa tattagaacg
    27961 tattatcgtg atggcagtgt gaagtttgac attcagttag cacgggctaa ccaactgacc
    28021 aataacttct tcttcctcac tgggcttcgc agcattctgg ccactcatac cgtcgtcaaa
    28081 aacgagattg gcaatggctt aaaccaaatg cgttacatcc ttaggcctta ttatcgtctt
    28141 aagccagggc ttaatatttt tacagagtac gaacatgatg aagaatatgg tgctcttaag
    28201 agaattcttc gaagtcaagg agagcctacc actcaagata caatcacgtt aggagtagct
    28261 gttctttttt aaacggtcac gtgagttgct tataccagga tactggagtc ctggtgcact
    28321 agataaaagt accggtttaa gcccacgctc ttgctcatgg gtttgaaatt attttgagtt
    28381 cagagtaggc caaataacaa atagttagta cccatggttt gtccagaaga aacctcgtga
    28441 atgcactaat tggaggaatg tatgtattta gatgctcttt cacggcttga taaggccgct
    28501 tcattttgtc gtattgattc cgaagcttta gagaaactga aacaccctaa atcctgtctg
    28561 gaagtatctc tcccagttcg gatggataat ggtgaattaa aaatttttac agcttatcga
    28621 gtccatcata atgacagccg tggtcctatg aaagggggca tacggtttca tccaaaattg
    28681 gatttagatg agataaaaac tcttgcatta tggatgacaa taaaatgcgc tgtcgtcgac
    28741 atcccctttg gtggtgcaaa aggtggggta attgttgatc caaaacaatt atctcgcatg
    28801 gagttggagc gattaagtcg aagctacatt gagttaatag cggattttat tggaccagac
    28861 aaagacatcc ctgctcctga tatgtatacc aatgagatga tcatgggctg gatgatggat
    28921 gagtatgcca ccattgttcg gcaaaatagt ccagcggtaa tcacaggcaa gcctatttct
    28981 ttgggagggt gcttggggcg tgagggtgcg accggtctag gtgcctacta ttgcattaaa
    29041 attcttgaaa aaaagaaaaa atggcagtct tctgagctac gagttgctgt tcaaggattt
    29101 ggtaatgcgg gacaaagtat tgctaagctg ctttacgaca atggctataa aattgttgcg
    29161 ataagtgatt cgaaaggagg aatttataat accaaaggca tcgatatacc tcgcatgatt
    29221 gaaataaaaa acagctctaa agaggtgcag tccatatatt gcaaagagtc tgtttgtaaa
    29281 cttgcgaagg atgctaccat tacgaatgaa gagttattgg aactcgatgt cgatttactt
    29341 atcccagctg ctgcacaaaa tcaaattacc caagaaaatg cagccagaat taaggcgcca
    29401 ataatcattg aaattgcaaa tggtccaata accttagagg ccgatgcact attgcagaaa
    29461 aagggagtat taattgtacc cgacatatta gccaatacgg gtggggtcat cgtaagctat
    29521 tttgagtggg ttcaaaataa atcaggttat tattggagta tggaaaaagt tcaagaagaa
    29581 ttacagacta tcatatcccg tgaattcaag aatatttggc agctgatgga acaatatcaa
    29641 acagatatgc ggcaagcggc atacattcat gccttgagtc gatacgataa agcagttaca
    29701 gcccaaggaa ctcgcggata tttttcaaat ttaagcctaa gcctataagg agagttatcc
    29761 tgcttgaatt tcatgcctct tcttactggg gctcattgtc ctattattgg tgttgattgg
    29821 ggtattaggc ttactctccc caaagattag aaggtccttc cccacgtctt tatgacggtg
    29881 actgatttac ctgactgaat atatttggca caaaaaaaac ctcttaacaa catgttaata
    29941 cagtaccacg catcaagaga atgagctccc ttcttcttaa tgtccgctgc catgcccgcc
    30001 ttgccagccg tgtccacctt gtccaccgtg atgccagttt tgttggtgcc aattttgatg
    30061 ccagttatga tgccacttgt agtggtattg aatccaatgc catctatgtc catcccaatg
    30121 ccacttacca tgtccaccat gaggtgtcca atgaggtgga tgatggacac tggcagccaa
    30181 tatcatagaa cctaattggg aatcattttt gactgaggta tcaaaagcag cggctacagc
    30241 agttgagcct atcaaagcgg ctacagcaac aaaccgcttc aacgtcttta aagtccacat
    30301 attgccgctc cttgtaaagg atgtgatagc gttatttcta agctagtcta aatccaatac
    30361 ttatcaatct aagtatagac aagatagcac tttagttcga aatttgaaca atatatgggt
    30421 gatactttac tggcgcaatc ttttatcagg atggactata ctaataaaat ttaatgagca
    30481 aaggagggca catcatgtcg cctgaaccta agcatcaccc gcagtcatca aaaccacaaa
    30541 aagagatgca cttttggagt tctccttctg gtatggtggc catcatcatt attgggatta
    30601 ttggctattt cttgattgta gagcatggtg ctcacattgc gagttttctt ggggcttcgc
    30661 cttttttgct gttagtctta ctctgtccac taatgcatct gtttatgcat agaggacatg
    30721 gaggtcatga agaggagcat catcataagt ctgataatga ggacgactcg tctgaggata
    30781 aggattaatc gaaaatggaa catacttcgt atgcctatgg tttgtggtta ttggtcattg
    30841 tcaattcggc tattttcatt ttgtttgcgt acagttttac caccacgttt aaaacaaggc
    30901 gagattggcg cgcctttggt gctttttctg cgtttgtcat cgcttatttt accgaaatgt
    30961 atggttttcc tttaaccatt tattttctgt caggatggct ttctaaatat tatcctggag
    31021 tagatttata cggtcatgac agtggtcatt tactccatac cctgcttggc ttgaaaggtg
    31081 atccccattt tgatgtcttt catatcttaa gttttgtctt cattatcggc ggtctttgga
    31141 tgattgcatc cgcctggggt actctttatc gtgctcaaaa gaagcatcaa cttgcaacca
    31201 gtgggctgta tgccaaaatt cgtcatccgc aatatgatgg gtttattctg gtgatgattg
    31261 gttttttatt gcaatggcca accattctga ccttaatcat gtttcctata ctggtttata
    31321 tgtatgttcg tctcgcacgt agggaagaaa aagaggtttt ggcggagttt ggtgaggaat
    31381 atcaacgcta cgctgcggtt actcctgggt ttatacctcg ttttggtaaa caacaagaca
    31441 gtaaaggagg tgcaccatga tgtttatgtt tcacagtgcc gagatgctcg gccttatcgc
    31501 aatagcgctt ggaatcatgt tggtcgtctg ggcattacgt aatgaaggca acagtactgg
    31561 attaagcaaa gtatttggat ggctgattgt cattattggg attttagggg aattatgcag
    31621 ttcttattac gcaattaaat actggtatgc aggctatttc cagatgccaa tgatgtcagg
    31681 tgaagcattc ctatttcatg tggctcagat gttgtctttg attgctatag cgtttggtat
    31741 tattttggtt gtctggactt cacgtcacga aggtttcggt atttcgttgg caaaagtttt
    31801 tggttggctc atcgttctct ttgcgatttt aggaatgatt tgtagtgctt attatgccac
    31861 tgtgtattgg cataaaggat atattcaaac gcctactggt atgcctctgt tgatgcaaca
    31921 tcaaataccg atgcagaaac agtcacccaa tcaatagtcc tggtaagtgc cataataaat
    31981 tgttatggca cttataattt tccattgtag agaaggagtc cctcaatgat gcttataaaa
    32041 cccaactcct ttaatatcag tccacaacgt ggtttggtgg caaaggaagc attgatcgac
    32101 acgggcttca tgctcatgag ccactttttt tgaaatcata ttaaaatgct ccatataatg
    32161 actgggtggc gcttggtggc attgagcaca atccattgaa ctcggagatg ggtgcctaaa
    32221 ttcagagagg ctccaatgag ccgtttcatg acaagcagaa caatcgtcgc caaataactt
    32281 aaagtgcttg tccttgtttt gatgacatga ggcgcagcgt aaagtcagtt cttgaggtga
    32341 tgaatggggg ttgacccgta atgtggactt attggattcg ttctgcgcta aaaagagcat
    32401 aacttgatta ataatcaact gttcttcttc atcatttgca tttgaaaagg cacgaagttg
    32461 ctgaagtcct aacgaaacca aagcattgtg atccatctgg gtgatacgtg ctgttctgcc
    32521 ctggtgctct ctatgacatt caacacaact gcctatgttg gcatgaaagg aggtcggctg
    32581 gcgttttaac agagattcgt tgttggcatg gcagacgtta cagttcgctg cctcaacccc
    32641 tttcacaggc gtatgacatg cagtgcagtt gtgacttaaa aacgagtggg ctttggaaag
    32701 ttctccgggg cttgccattc tttgccactc cgctacgttc gtcaaactgc tcacctcatc
    32761 tccatgagaa gacagataat aggccataaa agacagtgcc gcgattacgg taagggcagc
    32821 gagggtagcc caactcagct tcattgaaac caccttaatc caaaataaat tccagaccaa
    32881 acgtgcaaac ccagcagaac atacaatacc agtgctgtaa taatgtgaca ggttaaccat
    32941 ttttgaaaca ggcgcttgaa gtgctcgtga aaacgaatgg cgtattccag ctcagagatg
    33001 gcatctgcta aacacaaagc gcgtacttct gctgacttga ttaactgacc atccagtgcg
    33061 gtgtcttcaa gaagccagga aagaaagcga gctgtcataa gatgtaattt cccgatgttt
    33121 tgcttggggt gtgcgcttgc ttgtaattct tgagctacga tttggtattg ctgttgtaat
    33181 cccaagagga ttgtcttttt ttcatcgatt tcctgagtca taaagcccat gagataacgg
    33241 ccaataaaac cactgagcgt gacaatgagc gtcatagcag tcaaagcaat gcctagcgcg
    33301 ctattaaatt tatgtcctgt atgaatcaag actaaaatcg agccgataat acctgtgtaa
    33361 atatgccacg acaacagcgt attcatggac acatagtgtg ttacccaatt tttcaaaaat
    33421 gaaatgcgtt taacaaagag gtagaggagc ggaatcagca tcaatatcga gccactcacc
    33481 ccaaacacac caccccaaaa actcccggca aaacgagaag acgtatggac tgcaaagcct
    33541 agccatgtaa taagcattag aagtactaac cccaaaacca tcatacgtcc ttgcgcattc
    33601 attaagcatc cacctcaatg ttttgagtcg ctttggcctg acaggctaag ataagcccct
    33661 gttgtttgtc ttccttggaa agtgcatctt cacaagccat ggtcacttcc cccgacagca
    33721 atttcacttt acataaccca cattgcccgg ttcggcacgc attatcaatc gctatcccat
    33781 tggcttcagc gatttcaaga agggtacggt ccggcagaat tggaaccatt ttttcggatt
    33841 tcctaaacga aaccatcgca cgggtatcgg ctttgatgaa gtcttcttgg actatttctg
    33901 gtttcttttc tggtccaaat gcttctgtta gaatcaaatc agcgggcacg ttaagctctt
    33961 ttaagatacc aaggatagct tccatcatcg caggaggacc acaaacgtgg atacgatgag
    34021 aggcaatatc agggacaaga tggctgatga tattcttggt aaataggcct tgtaacccca
    34081 tccaaacggt tccctctgaa cgcagcatcg acgcatacac atggagattc aggtatcttt
    34141 cttgaagttg ctctaattct tcgcgaaaga taaactcact cgtcgtgcga caacaataca
    34201 gcaaataaat gtcattgtgc catccaatat cggtgagata gcggatgata ctcatcatgg
    34261 gggtaatgcc gaccccgcca caaatcagta caatgctctt tgcctcttcc ccagtaaaag
    34321 taaatttacc attaggtccc atcacctcca gtaaatcccc ttccttaatt tcgtcatgca
    34381 aataacggga aaacacgcct tgttcttccc gcttgaccgt gatggcacaa taatgcaatt
    34441 gggttggggt ggacgccatg gtgtaactgc gtcttatcgt tttaccatta attaaggcgg
    34501 tgagcgtaat gaactggcca ggataatagg taaatggcaa agccacctca tgggttgatg
    34561 ccaaatgata cgttttgatt cctggtgctt cttgaaagat acggcaaaca cgaagttgtc
    34621 cttcccattt ctcttccggt tcggtgagcg atctcatgac cggaaattgg gggactggac
    34681 atttatgcgc ggggcaggta ggaggggtag ttggggctgt ttctgatggc actttagaag
    34741 cttgagagga tttaatgggc tccactgatg ttaaggaagg ggttggttca ggtgtttttg
    34801 agaattgggg tgaggtatcc aaaggagcag ccgtattttg ttggattaag cgatcgagca
    34861 aagcagccgc tcttctcatt ttgaaaaaat acatccaaat catgaccaaa aaaaacagag
    34921 caaatagcgc aataatcgtt aaatgaaaaa aaggcccccc taagacattc tcggcttgcg
    34981 gactagttgg aagaagattc atttctcgtt taaaccactg caaggcgatg tcgcttggat
    35041 tttttccttc agaaagggcg cgaagtgcgg ccagtgcact ctcatattgc gcaatccctt
    35101 ctcgtaattt ctctaaagct tcctgcattt tagcgtaatt gttagtgctg gatgccaaca
    35161 aaagctcgtt aagcccacct ttcatgagcg cgatgcctga ggtgatgcgc tggtgtgcct
    35221 tttgttgaat ttcaaggcgt ttttcagggg ataatgtggg taaactcatt aaagaagggt
    35281 aaagctcctt agagggagtc cctatatttt tcatcatgcc actcattccc tcgccttctt
    35341 tctccttttc tcctggatga tgtgcggcat gctcatccgc tgtgggttgt gcaaaggaaa
    35401 cagaggccat cgccaacagg aaagaaatgg ataatgttcg aacccatcga agtaaggtaa
    35461 actttttagt gtattgaaac agcatccttt ctctccaaaa gctcacttaa catgacgatt
    35521 caaaaaatgg ttaaagtgga gcagtctgca cgagtgtttt cccgcctcat atgagtttat
    35581 tcactattct gtaaccgatt tccaatcact cgaaagggat gactacgaat ttatagaaaa
    35641 ttcatttcat tgattagaaa cccagtccaa tcagtatatt atagtttacc ttgtctttct
    35701 atgtgagctc atgaccaata atcgcttcgg cgctttgtac aatattctga atttcttcgg
    35761 taatttcttc ttggcgcagt aaattgagac gatggtttaa tgcatttttt ttattttcta
    35821 agcgatctaa tgcctggttt aagtgaaaca gtctttgatg attctccgca aaaaacgatt
    35881 gatataaaat cgaataacac atggccagta aataatgctc caccaagtca gcgaaaaact
    35941 ggtctttgga accattcaaa acgggtggca cagaaaaata aggcgcagga cttgtatcca
    36001 actctttaaa gggttgccag gtttttgctt gaatttgatt gtgctcttcc tcattgaaga
    36061 gaatagtcca ttgccaagaa tgccatgtct tatttctttg caataaggct ttttctaatg
    36121 tttgtaacac gtttgaaatc acgtctggga tttcttcaat ggcattgggt ccatcgattg
    36181 ttgctatgac acgagggtca ttaggcattt ttaatgccag tttatgccct acaagaatga
    36241 gtgctggttc caattcagaa tggtgctctt tgtgcacgtc taactgatga agcacattat
    36301 cattaaaaga accacaaaag ccgcgttctg agccaataag aatagaaact aagggaggat
    36361 tttcttgttg attcatgggg ggcatgggat agaaacttaa aaaatcatga ctcacctcac
    36421 taatggtttt aatgacgttg tcctgcatgg aaagacattg agtgattttg cccagttcaa
    36481 ttaaagaaag atttttcatg gccgtcataa tgtggccaat ttcttctaaa gtatgaagat
    36541 gttctttgag tttggttcgt ttcgtcatga taccatccgg gtattaagtg atactcattg
    36601 ctttggagtg gataatgagt ccgttctatg ccattagcca ttcttttatc gctttttccc
    36661 attgttctcg tgggtttccc aaggataaag tgctctgttt tatttctccc tctatttttt
    36721 tgagaatttt ggggatgtct tctaaattca attcgtcaaa gaaaccctca ttgtaagcaa
    36781 ttagccatgc gagttgaaat tcaatgggta gtggtgaaaa acgttcttgt tttagaattt
    36841 ctcttaaaat tctccctttt tgaatctgct tttgcatttt ggcatccagt ttcgccccaa
    36901 agcgggtgaa cagttccaaa tcaagaaatt gcaggtaatc aagtttcatt cgaccggatt
    36961 cttttttaat ttgtggatgc tgcgctttac caccgactcg tgagaccgat ttggtgatat
    37021 caatggcggg aagaaatcca gaagagaata aggattcatc aaaaaagatt tgcccatcgg
    37081 tgatggagat gagattcgtt ggaatataag tagccatttc accttctttg gtttcaataa
    37141 taggcaacgc ggtcatactg ccgccgccca tagcagggga aagacaggtg gagcgctcta
    37201 ataaacggga atgcagataa aaaatgtccg caggaaatgc ctcacgtcca ggaggtctac
    37261 gcagtaaaag ggacagttca cgataactat tggcatgagc actcaaatca tcatagacca
    37321 ccagggtatc taagcccttt ttcatccagt gctcggcaat ggcgcaccct gcaaaaggag
    37381 ctagataaag taatcctggt aaggccgtcg cttgggccac cacaacagtc gtatagtcca
    37441 aggcattggc ttcttttaat aattgaatgg tagagcttac tgtagaacgt ttttggccaa
    37501 tcaacacata gacacaatac acatttttat ctttttgatt catcacaatg tcaagggcta
    37561 aagcactttt accaagccca ttatccccaa tcagtaactc cctttgtcct tttccgatgg
    37621 gaattagatt atcaagcatc ttatttcccg tgtacaatgg tcgattcaca aagtcgcggt
    37681 gaagaatagg tggggacagc ctatccagta atccttgttc ttcatggggc ggaatctcac
    37741 cgccgtctaa tggatggcct agaggatcaa tcacccgccc aagcagttta tcgcctactg
    37801 gaatactcaa tacgcgtttc agaggaaaaa taggggtgcc tgcctttaat tttttggttt
    37861 gtaccagcat cacagcgcca accagatctt cggtgagatg gaacaccatg gcaatacagc
    37921 actcatcttc tgaaataaga atttcatcga tggcagccct tggtaagccc ttaatccaga
    37981 taatcccatc tccaacagaa accacccgcc cttgttcgga taccttgatt tgaaattgat
    38041 agcgctcaag gcgttgtcgt tgcttctcaa gaaaagaagg ggtgttaggc caattcattt
    38101 tttgtctctg taaagaattt taattcatca cgaagattgg cttgcaagca catggggccc
    38161 atttgcaggg ttaatcccgc gagaagcttt ggattttccg tgaaacagac ggtaagtttt
    38221 ttaggcgaca attgttcaat gacttgcaag agatcctgtt tctgatgttc ttggatgggg
    38281 taggccgttt gtatcgaaat ggtttcttct tccggcacag tattgagcca ttgccattgt
    38341 tctaccggaa aatgattcag atcctcaatg gtttttttaa tgatgttctc ttcaagatgg
    38401 gcatccgcaa aaggcattaa caatttttca gcaaatttcc cagccaatag aaacgcttcc
    38461 ttggcattgt tttcaatggt ggctgcggct ttttgcattt catgggaaaa aacctgctct
    38521 ttttcttgac gcaattgttt ttcaaaccgg agtctttctt ctgatttcca ttgctccatc
    38581 gcctcatgcc actcgttttg tagggtcgct ttctcttgtt gccaatcggc aaggcgatgt
    38641 tcataggttg tttgcaattg cgttgcttgc ctgtgcagtg tttgtgcagt ctctaattgt
    38701 tcttgcactc ttttttttcg ttctaatatg gttttttgta tgggctcata aagaaagcgt
    38761 ttcaaaatcc aaatgagaat aagaaaatta atgagttcta atgaaaaagt ggtccaagag
    38821 agttccattg gttttatccc tgttgcgtta caaaataaga caataatgga tttctaaaga
    38881 ggataatcaa aacaatcacc aaacaataaa tggctaacga ctcgatcatg gccaaaccaa
    38941 taaataaggt tctggtgatg gatttttcgg cttcaggttg ccttgccaaa gcatctaagg
    39001 cgtgactaat cgcgcgcccc atggccagag ctggccctat agttcctatg gcaatggcta
    39061 ttgctgcaat aacggttgac gctaaactaa accaacttat gtcattcata acgattctcc
    39121 ttggctttta agctcatggg cttgaatgcc cccagcaatg taaattaagg ccagcatgcc
    39181 aaaaatatac gcttggatga tggcttcaat aatatgtaaa atgagtattg gaataggaac
    39241 taaaaatccg gcaatcatga ggacaatcag ggcggttaat tgcaagctca tgatattacc
    39301 aaacaagcgg acggccaatg ctaaggttcg agagatttcg ctgattaaat gaaaaggcaa
    39361 taaaaaagga gttggcttaa tataatgttt caaatactct cgccacccct cggcacgaat
    39421 tccaaaccag tgtaccgata aaaaagttat tatcgcaagg gatgccgtca ccgataaatc
    39481 cgccgttggt gaataaaatc ccgggataac accgattaaa ttggaaacaa ggataaaaat
    39541 ccacaaggtg gctacaaaag gaaatatcaa ctcaacgtgg ttgggcaata cttcttttat
    39601 cgcatcgtac atggtgctca aaacaccctc ccaaaggagt tggtacgtgc ttggctgtag
    39661 gagagagcat ttgtaagtgg tactccatgc catgatgaat aaggagatca taatgaacca
    39721 cgtggttaag acgctttgtg tgatgggtaa tccaccgatt gaaaaggcaa aatgcgctaa
    39781 gaatccttct tctcccacgg ttagtcctta attaaaagat acacgttaat ggcaccaata
    39841 atcaccccaa ggaggattaa attgatggtc caactcatgg aatacccttt tattttctca
    39901 tcgagccaaa ccccgagata ggctcctgca atgatgggta acacaaaaac aaagcctaaa
    39961 gtacccaggt aaactgtttg ggctaataac gttgattttc cctttttagc tttattgatt
    40021 ttgcggacgt ttcgtttcac ttgttgttcc agttccttgt ttgaattttt aaattccata
    40081 gctgttctgc cggtttaatt cccataaacg ctttaacatt tcttcatcca aacgatgcac
    40141 gctttcttta gtgcgcaata gattgttttc ttcccaatgt aattcttccg ccaggaccct
    40201 ttgtatggct tggtattctt tatggcgcac ataatgtcgt gtcgcaatat gcaattgatt
    40261 gtcaatgaaa taaaccacgg cacctggcaa tgctaagtat tccgtctctt gattttcatc
    40321 acgaaaccag gccaagccga attttaagca cgtcatcatt cggctatgat gagctaaaat
    40381 tccaaattgc ccagaagcat cctcacccac aaaactgatg accttatcga ttttttcata
    40441 ttgtgtggca ctcttaagat ggatagtaaa aagctccatg tccgtcgtta tctcccttta
    40501 tccatggcac ctctcatgta acactcttct tctgaaaatt catcgtaatc gcctcttaga
    40561 aaggattcgc aatcggttaa tgtgtgttct aatgaaaccg atttcccttc cattcctgtt
    40621 tgaagcttgg tgacatgaaa gggttggcta agatagcgct gcagtttacg tgctcttaac
    40681 acaatagcac ggtctttagg agaaagctct tcaatcccca tcatagaaat catgtcttct
    40741 aattcttgat aatgttcaag gtgttctcgt acggcttgtg caatggaata gtgtcgctcg
    40801 cccaatagga ttttatccat aaattgactc ttggaagcta agggatctac ggcgggataa
    40861 atgcctttgc ctgcttgtgc gcgagagagt acgatgatgg aatccaaatg agtaataatc
    40921 ccggtcaccg ccggatcgct catatcatca gccggaacat aaaccgcttg taccgaagtc
    40981 actgctcctt tggcagtcga tgtcatgcgc tcttcaagtt ccgctatctc agtcattaag
    41041 gtgggttgat aaccgacact ggccggcatt cggccgagta ggcctgaaat ttcactgccg
    41101 gcttgcacaa aacgataaat attatccaca agaaaaagaa cctcatggcc caacgtatca
    41161 cgcaaatatt ccgcataagt taatgccgat aaccctgtgc gaaaacgcac tcccggtgat
    41221 tcgtccattt gaccaaacac catgagggtt ttatccatta cgcctgcgga tttcatctca
    41281 tgccacaatt catgtccctc ccgaatacgc tcgccaacac cagcaaagac ggaggttcct
    41341 tgatgcaatt gaataatggc atgcatcaat tccatcagga gtacggtttt tccaactcct
    41401 gcaccaccaa acaatcccgt tttagagccc ctgacgaagg gacaaagtaa atcaatgacc
    41461 ttaattcctg tctcaagaat ggtttcttga gtgctcgtca tttgaagtgg ttctgatttg
    41521 gctagaacat cacgatattc attggtttct aatggtgggc cgccgtctaa cggctcacca
    41581 aaaatattta aaaggcggcc caagcattct tttgaaacgg gaatttgtaa cgaagtgcct
    41641 tggtcataaa ctattaaacc tctttgcaat cctgaggcac ggtggagcgt gatggctctc
    41701 acatgatgct catcaaggtg ctgacacact tccaaaatgt attcatcact gtccgtgtaa
    41761 gttttaagag attgatgcaa aggaggcagt acatcacaat gtatcctgac aacagggcca
    41821 ttgatttcga ttatcctgcc gatgggagtt ccatgacttt gagcaacagc cgtgttttta
    41881 tccatcattt tccaattgta ttcatgaccc atgaattagg tgtcgtccta ttctctctat
    41941 ttttacatca ctttatggaa ttttaatata acagagtttg aaaacagaga gcaaaatgct
    42001 aattaataac agcccgcctc aaatctaaaa aacaaaagct atgtaggagg tctcatcaat
    42061 ctgcacaccc atgtcactgg gttatatgat gatacaccaa accatccctt tatagagttc
    42121 aatgaaatag gtaggctctg aataggccaa tcttaatggg ctactgacaa tagggctctt
    42181 agacatctca taagagctca ggtgatttga gtcaattacc ctaggatagg cctctcttat
    42241 agaggagtta ttggtttttc atcatgggac atcccatcat ttgcctatcc atcatcatct
    42301 gcatcatttg cgtcatcatt tgcatcattt tcatacgttg atccatcatc tcgggttgtt
    42361 ttgctgtatc atcgtgcata ctttgcatca tcttcatgcc tttttgcatg gtgtgcatgc
    42421 cttcttccat catgggcatt tgtgtttgca tcaacgattt cttttcttcc gttgtttttg
    42481 cattcataag cttatcatgc atctcctgca tttgttttag tgcttcttgc atttgcttca
    42541 tttcgtcttg cagttgggtt gactgctgat tagagctttg cattcccata ttattcattt
    42601 gagaaccaga aggcatatct tctgcgaaga gtggcgtggc aaccaagctg caagcggcca
    42661 ataaaatgaa cgcattcctt tttaacataa tgaaatctcc ttattatttt ttcaatgaat
    42721 tgtttggact gaacatccta ctatagcact acatgaacag gggatgaaaa gcaaaaaaca
    42781 tacttgttca tgttaatgcc aacctattcc tcccgtgttg tgtccttcat aagtatgaga
    42841 aacacggtgt gaattattgg tcgttgttga tggggcgcaa gagatcaaca aaagtatcgc
    42901 gctcattatc ccaataactg ctttaataag tctctgcttt ggcatatcta tgctccttgc
    42961 aatgaaataa agtgagagag aatcaatata aaaacaaccc acctcatttt ataaatcatt
    43021 tatataatat agcaccgttt agaaatacgc agtgccagtc atgactactt gttgaaaaaa
    43081 tcagttgtct tttggaatag ggaatctata cctccccaag cttaatataa gttcaaatta
    43141 ttatttgaat tcactgccaa ggatgtgaat agatgttgga gttaaggaag acgataagta
    43201 gggtatttaa ttattgcgaa gattctacca ttcttttatg aagaaatttt aagatgattc
    43261 aaaaggtaac atgccagtta agaagcgatt tggtggctat catcaccaca gacatgggca
    43321 ttgacaccat tagaaatgat ccaatgaaca ctcattatta gtgataatta atgcctgata
    43381 tcgtatgaaa atattgagta tacgaagaca agtacttttg ttataacttt attaattttc
    43441 cttatcctgc aggcattttt tacatgggtt tttatttcaa ttttccactt ctttatcaag
    43501 cattagatta gccccttact catcatggtt tatgaggctg agtttttagt tattttcctc
    43561 ttcaagctca cataagttga gggcgggaac aactcatttg gtgtgacatc taatccttta
    43621 agaattttta gaagatttaa aatggttata ttggcttcgc cacgttccac agtcccgtag
    43681 taaccccgat tcatttcgat gaaattggca aaaccctctt gagaaaaacc tttctctttt
    43741 ctgattttgc gaatttgctt gcctagtaca attaagtcgg tttgtttttt cattcaaaaa
    43801 gcatatgtgc tatgcttcat atatagccac acggtatgtc tatcattttt gagttttgtt
    43861 taggaaatgg taataaaatc ctattttgtt atttttaaca ttgttaattt ttaacacggt
    43921 aaataatcgt tatttgaact atattgatac gattaggaga agaaaataat acctgactca
    43981 ctggatagtt atcaggaatt aaagtaggtt agagttcttt agttaaattg agcggactca
    44041 tgaggaattc atctgtaggt aaaccaattt tagtattgag aaatgacgtg ctacttaatt
    44101 aaaagggatt taaagatttg ttttatgctg gagaccatag atgaaaggta tcgtttttac
    44161 ctccttaaat aacatgatta tagaacaatt tggcatagaa acctgggacc aactcgtatc
    44221 ctcactagac cttccaagtg gtggaagtta tacagcaggc ggcacttact cggatacaga
    44281 acttcagcaa ttgattcagg ccattgcgaa gaggaccaat cagcacgctt ctgttttttt
    44341 agaggccttt ggtgaataca tgtttcctat cttatcgagt aagtacgcaa tttttttaaa
    44401 aaaggacatg acattaaaag aatttttaaa aagcattgat ggaacaattc atgtggaagt
    44461 agaaaagtta tacccagatg aaacattacc taccattagc tatgaagagc ctgctgcaaa
    44521 acaattggtt atgttatatc gatcacatag aaggctctgc cattttgcaa taggtctcat
    44581 ccaaggagcc gctcaacatt ttaaaaagag aattaccatt aagcagactc actgcatgtt
    44641 aaaaaaagat gatcattgtc gtttggagat tacctttgag taatgagaac aaagcaatat
    44701 ttgagcgctc ttattatcgc gaaagaaaag cacgcgagga agccgaatta ttgttggaaa
    44761 ataagacaag ggaacttcat ttactgaacc aggatttgga aaaaaagaat gctgaactgc
    44821 taaaaacgct aaaagattta gaaagcgtac aaaagaaatt atcgaaatta gcccattttg
    44881 atgtattaag ccgattaccc aatcgattgc agtttgaact ggatttaaaa cgtgagattg
    44941 ccaggtcaaa aagatacggt cgtcatctgg ctctgttaag catcgatttg gattttttta
    45001 aaaatgtgaa tgatcgcttt ggtcatgatg tcggtgatgg tctattgcgt gaagtagcga
    45061 agcgtctttg ctcaagtctt cgcgaagaag attgtgtggc tcgattagga ggagatgagt
    45121 ttgctgttat tttgacagaa attaataatc cacaacaggc aaaattagtg gccaatacta
    45181 tgattcaaaa gatgggtcaa ccattcctta ttaaaggtca taccgtcatg attggactaa
    45241 gtataggaat tgcttacttt cctgatgccg gtgaggaggc agtaaccctt cgtaagaatg
    45301 ccgatattgc tttatacaac gccaaagaat gtggtcgaaa taattgccaa atctttacac
    45361 cgtctcttag aaaacaatat tcaaaacgct tgggaatagg gaccgagttg catcttgctt
    45421 tggagcgaca agagtttttt ctcgtttatc agcctcgggt tgacttaaaa acgaattcta
    45481 tggttgggat ggaggtctta ttgcgttggc aacatcccaa gcgaggtgtt gtttttcctg
    45541 atgaattcat ttccgttgca gagcatttta acttgattat tccaattggt gaatgggtct
    45601 tacgaaccgc gtgtaggcag tatgtggatt ggaaaaagag ttacgctcct ttcaactgca
    45661 ccctggcgat taatatttcg cttcatcaat ttcaacacaa agggtttatt tcttctgtga
    45721 agcacatttt acaagaatat caaatgccat cggattggtt agagcttgaa attacggaag
    45781 caacggcagt caattttttg ggaaagattg aagatacatt aagtacgctt cgcaatatgg
    45841 gggtaaagct tgcttttgat aattttggct caggctattc ttttttatct cacttaaaaa
    45901 attcaccagt ccaatccatc aaactgaaac aaaccttaat tgaagatgct catctcagag
    45961 aaaatgataa tttaattatc aaatcgacga ttgctttgtc gcaagaatta ggattgaatg
    46021 tggtggtggg tggtgtcgaa acagaagaac aactcaattt tttacggtca agtcactgtt
    46081 tcgaagtgca aggttattac agcagtaaac cgttaacagt aggacagatg acgcaattta
    46141 tcgaggaaag gaactccaaa gggctgtagc tttatgttat ttcattccta tcaaaaaata
    46201 acaccaatta ggttaccaaa aataatttag cctaaaatgg ataagttaat aagccccgct
    46261 ttattgacca aaatctatga gaaaatgttt cttgtaggaa agtaggagcg ctgtttggtc
    46321 aaggagacag caaggggtga ctatttagcc tttctatttc cgaagagaga ctccttaaac
    46381 ggaattgatg ggttaaatct tgtgctatgc tgtattatat cgtgttgatt atagggcata
    46441 atgccaggga tgtcgtggcc atgaagagat cagaaaaaca atgttgtcct aaggaaagga
    46501 tactcactca gagtcaagaa ttgcaagaag gggtagattt ctcgtgttgt gcacaggctg
    46561 atagaggtac ttccgataac ttctttccat ttttcactcg attagtgcaa gccaacttgg
    46621 ctaaatggac ggcaggaata agtcctgctg ccatcggctc ttcttattcc acctggcttt
    46681 ggcaattggc ccaatcccct ggggttttgt gggaacttgc tttttatccg ttttttcatg
    46741 ccaaggattg cattaataat atcgtttgtg ttgaacgtgc tgcggatggt aaagacgtgc
    46801 gttttaaaaa agacagttgg cagcccatgc cttggcgttt attcgccgaa ggatttttgc
    46861 agatggagga ttggtggcga cgcgccacaa cggacgtacc aggattgccc aaccaggtgg
    46921 aacgtattgt ttcgttctgg gcacgtcaat cccttgatgc cctatcccct tccaattttg
    46981 tttggtctaa tcctgattta ttccatgaag ccaggcgcac tgggggcctt aatctcatcc
    47041 aaggaggcca aatcgcattc gaagattggc tagaaaagct aaccggtgca ccacctacag
    47101 gttctgaaca ttttatccct gggaaacaag tggctattac cccagggcgg gtggtctttc
    47161 aaaatcattt aattgagctc attcaatatg aggcccaaac gaaaacagtc tataaagagc
    47221 cgatacttat cttaccagcc tggattatga aatattatat tctcgattta tcaccacata
    47281 actccttagt gaagtggctc gtaagtcagg ggcacaccgt atatatcatt tcttggcgca
    47341 atcctgacaa agaggaccag gatttgggga tggatgatta ttatcggcaa ggagctatgg
    47401 ctgcatttga tgcggtgtcg acccttttgc cagaaactaa aatcaatctg atggggtatt
    47461 gtttaggggg caccctggcc atgattaccg cagctgcgat ggtaagagac aaggacgaac
    47521 gtttaaacag cttgaccctc gtggcagccc aaggggattt tactgaggct ggggaattaa
    47581 tgctttttgt gactgaaagt caagtggatt ttcttaaaag tatgatgagg gagcaagggt
    47641 atctggatac caagcaaatg gccggttctt ttcagatgtt gcgtgcttat gacttaattt
    47701 ggtccaaaat ggttcaagat tacatgcatg gcatgcggcg cgggatgatt gatttaaccg
    47761 cttggaatgc ggatgctaca cgaatgcctt ataaaatgca cagcgaatac cttgaaaaac
    47821 tgtttttaaa gaatgatttt gccgaagggc gttacaccgt tgaaggaaag ccggtggctg
    47881 ctgaaaatat tcagttgcct gtttttgcgg tgagcactga aaaagaccat gtagcccctt
    47941 ggcaatccgt ctataaaatc catcttatga cagaagggga tgtgaccttt gttctcactg
    48001 gcggtgggca taatgcgggc atcatcagtg aacctggcca ctcaggacgt tcttatcgtg
    48061 ttcatgagca taaacaaggc gaggcttact taaatcctgg gagctggctt gcgatggcag
    48121 agaagcggga gggctcctgg tggcaagcat ggcatgcgtg gttagtccag caaaacacta
    48181 aaaaacgcat tgcatcatcg gtcatgaatc cctcactgcc agcggcaccg ggaacttatg
    48241 tgcttcagaa gtagagcgtt cttcttgaac aataaaacag gagtcaagga cggatgaaga
    48301 gtaaaaacgc ttgattgtcg attttgctaa tccgcattct attggtgctg ctcaataggg
    48361 caatgaattg gtcattactt atcaaaattg aaaaaccagg gaataggtac aaatttttag
    48421 ccccccattt tcatgccgct ggatattatt aagcatctga taaagccgca cccattgctg
    48481 ggcaaatcct ttatgtggat ggcggcttta aaatataaac gctcaatgga ctttcgagtg
    48541 agttacatgt attgcccacc attgacatca aaattggcgc cggtaataaa accactttgc
    48601 tcttctgcaa ggaaagcaac aacacgagca atttcctggg gtttgcctaa tcgtcccaca
    48661 ggaatttgag caacaatcgc ttctaaaatg tcatccctca gagaagcaag catctcggta
    48721 ttaatatagc caggggagat ggtattcact gtgatgcctt tctttgcgac ttcttgtgcc
    48781 agacttttgg taaaaccgta taatgcggat ttagtggccg cgtaattaca ctggccaaat
    48841 tgacctttgc gtccattaac ggaagaaatg gtgatgatac gtccataact attatccaac
    48901 atggtggata tcacggtttt cgtcatatta aagacactat ttaaattggc atggatgaca
    48961 ttctgccatt gttcttgagt catctttttc aaagtggcat cgtttgtaac accggcatta
    49021 ttcactagaa tatcgatctt gccgtatttt tcgatgacca attcggtcac tttctcacaa
    49081 tcattgaagg aagaaatatc ggcgtagaca atgtcgatat caaagccttg catggcctgt
    49141 tttttctgcc attctttcgc caaatcatga ttgccttgtt taaaataaca ggcaatgact
    49201 tgataatcta acttttgcat ttcctggcat atggcagtac caataccacc agtgccccct
    49261 gtgactaaag caactcgttg attcacttct ttcctccttg atggtttcat acagtaaaaa
    49321 aataagtgtt attgtcatca gaacggtttt gcctagatct aatagtggtc atttattaga
    49381 taacttctac ttcctcggcc tgaggtcctt tctgtccttt tcccatttta aaaagaacag
    49441 ttgccccatc aggaagtgtt ttaaagccgt cactttgaat tgaactaaag tggacaaagt
    49501 agtctttgcc actgctttca ataaaaccaa aacccttgtc tttattaaac catttgactt
    49561 taccacgaat tttattggcc atttttgttt atttccttat aatcaagcag gttacatagt
    49621 gtcatgccac gttgttcttt ttgtcaatac ttgttaacca gagagctctt agtgatttaa
    49681 tggattgaat cagtggactc tccagtgttt ccctttgttt ttttcaatga aaccacttta
    49741 tcacattgaa tctcaatggt atttgtttta gcagcagata ctccccaaca tcgtattctt
    49801 tttccctctt tctcgatgaa tagcacgtga ataaccaaat ttttcccata agtgttgtcg
    49861 atggtgatgc cttgagtctc attcgttttt gtcaggagtc ttcccagtga attgccacta
    49921 cggtggaact ctattaatac ttcagtattg gataaggaaa cattgtttat tttggagtct
    49981 ttgtggtatt tgatgtgata ggattgcaag ccagcatagg cattgagtgt cgtgcaggaa
    50041 aaaattgccg caaaagtaat tttaagaaga actatttttt ttattttaaa tgacttgata
    50101 tcgttcatgt gattcctatc cttatctttg tccatgtact tttactattt tgtgtcataa
    50161 aatttttaca tgcccaatct ttaatagcaa acggattatc tgcttagac
//
LOCUS       DAXLDT010000007        41918 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXLDT010000007 DAXLDT010000000
VERSION     DAXLDT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 41918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41918
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.007"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            334..624
                     /locus_tag="ACF2SP_000318"
     CDS             334..624
                     /locus_tag="ACF2SP_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2358133.1"
                     /translation="MNTFNIEEASKFLGAHRETIRRMAASGELPGVKIGRGWRFIEQD
                     LAMYMRNKYSTCDASWGVDIGDNKWHSRKKMGFGGSTSPSVTSVYAEALGLK"
     gene            537..1541
                     /locus_tag="ACF2SP_000319"
     CDS             537..1541
                     /locus_tag="ACF2SP_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2358134.1"
                     /translation="MALTKKNGFWWIDVTFGDKRIRRSTRTKVKQQAQQFHDNFLQDL
                     WSNKHTKLPQQKSWVDAVVRWLEESAHKRSLVTDKFHLAFLDPYLGQKTLDEINAELI
                     EFIAQEKERTNVTPTTVNRMLEIIRAILNKAHKEWQWINTMPLFRMRKVENKRIRWLS
                     RGQANKLIQELPNHLKDMAAFTLATGLRQSNVTQLQWSEVDLIRKHALIHPDQSKTKK
                     AIPVPLNSQAIVIIEKQVGLHPTFVFTYFGKPVTRCNNHAWQKALIRAGIKDFRWHDL
                     RHTWASWHVQNGTSLQELQQLGGWSEYEMVLRYAHLSSDHLKVAAERITKNSGTFSSL
                     "
     gene            complement(1606..1682)
                     /locus_tag="ACF2SP_000320"
     tRNA            complement(1606..1682)
                     /locus_tag="ACF2SP_000320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1646..1648),aa:Arg,seq:tcg)
     gene            complement(1741..1816)
                     /locus_tag="ACF2SP_000321"
     tRNA            complement(1741..1816)
                     /locus_tag="ACF2SP_000321"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1781..1783),aa:Lys,seq:ttt)
     gene            complement(1872..1947)
                     /locus_tag="ACF2SP_000322"
     tRNA            complement(1872..1947)
                     /locus_tag="ACF2SP_000322"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1912..1914),aa:Lys,seq:ttt)
     gene            complement(2034..2912)
                     /locus_tag="ACF2SP_000323"
     CDS             complement(2034..2912)
                     /locus_tag="ACF2SP_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFD2358135.1"
                     /translation="MKWQIPAKTFLLGEYAAIAGEPAILLTTSPCFILTLTENAKLEG
                     IHPESPAGLWWQKNQCYPFGLSWQDPYHGQGGLGASSAQFLACYLATCSLQEITPRLN
                     HMLEAYYQSSWSGLGLRPSGYDVIAQSQQGCVYIDKQKNQVNSFSWPFKDLSFLIIHT
                     GNKLATHHHLQEVTLPSQIDYLASLVIKAKEAFDLAHSHHLIQCINLYAQKLTELNLV
                     SQHSLPLIQSIKSYPETLAIKGCGALGADTLLILTDKRDLQSLKDKVQDQNWLILATE
                     DNLTVRNNNSLFEKSI"
     gene            complement(2909..4207)
                     /locus_tag="ACF2SP_000324"
     CDS             complement(2909..4207)
                     /locus_tag="ACF2SP_000324"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFD2358136.1"
                     /translation="MSIAANAGELFRGFSKLSREERFQRLCALGALTHEDITFLKQGG
                     IKDLNLADKLIENVIGYFQLPLGVATNFNIDGRDYVIPLAVEETSIIAALSKSAKWIR
                     QHGEINTWVHGECILGQIQLAKVKDFQRFSDLFNKNRQYFIEIANKDVAANMVKRGGG
                     VTDLQVRHLKREDGQDMAVIHLTMNSCDAMGANIINQVLEYLKQPIEQITGEEVTMCI
                     LSNLNDQKLTTAQVIIRNIDPILGQKLQEASLFAEIDPYRAATHNKGVMNGIDPVLIA
                     TGNDWRAVEAGIHAYAARSGQYKAITRWRYQNEILTGELTAPIIVGTVGGVTSLHPTA
                     KMCLRMMDITSANQLSQVIAAVGLVQNLGALKALCTDGIIQGHMKLHIDNLLLVAGAN
                     ENEMPVLKEKLQEWLNMNKRVSLNNAYDLLAEIRQAPVAV"
     gene            complement(4198..5226)
                     /gene="fni"
                     /locus_tag="ACF2SP_000325"
     CDS             complement(4198..5226)
                     /gene="fni"
                     /locus_tag="ACF2SP_000325"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFD2358137.1"
                     /translation="MQNEYSQFEQRKRDHIELALMPANQSSELNPFDHFSLVHEALPD
                     LDFKDISIQSIRFKKPVEKPFIISSMTAGHSNALEINYRLMEACSKTKWAMGVGSQRR
                     ELTDKQAAFEWAPLRRDFPMVSLFSNLGIAQLIDTPISAIERLIDTLQAEALIIHCNP
                     LQECIQPEGTTNFQGCWAALEALVKKIASPVIIKETGCGFSKNTLLRLNNIGVAAVDV
                     SGVGGTHWGRIEGHRANKDPIRHRTADTFRNWGIDTLQSIRNAISLNPSFETWGSGGV
                     RNGLDAAKLFALGATTVGFAKPMLEAALDSTGQVLTQMNTIEYELKTAMFCTGSRVLD
                     DLKEKACP"
     gene            5394..6445
                     /locus_tag="ACF2SP_000326"
                     /pseudo
     CDS             5394..6445
                     /locus_tag="ACF2SP_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2050 family Dot/Icm T4SS effector"
     gene            complement(6583..6882)
                     /locus_tag="ACF2SP_000327"
     CDS             complement(6583..6882)
                     /locus_tag="ACF2SP_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358138.1"
                     /translation="MGRFIFKGTSLIKLMLAFTLLIGALILVTPANAKAVNRDINPII
                     NKANPTQMAYWVRYRYWGPYYYRGYYYRPYYYRWGYPYYRPYYRPYYRPYYYYRW"
     gene            complement(7009..7833)
                     /locus_tag="ACF2SP_000328"
     CDS             complement(7009..7833)
                     /locus_tag="ACF2SP_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358139.1"
                     /translation="MNEKLIETQSNLIKQAEAELTQSNQLLESLQQEKGLQEESDLDE
                     LDSLKSLILVLQYRLDTQNALSQLGHLQQYLLQLLGIEAKHSFEFNLERLAFALGTDE
                     LKQILAMLNHLVESLLRVIAQKQLKESHDKRKSLEQTKQFKPIDTLVKVLNKQKLFMA
                     KLEELKLVLENIGGAPQPGIIYDHIAALQGPVSRFYQALQHGLGLSNSLYQQLQKKNQ
                     LSYQLPDTLLKPEHVIRNMNFHPESQRLFKTTLERNKLENLEERAAAKRLGHFFNH"
     gene            7954..9078
                     /locus_tag="ACF2SP_000329"
     CDS             7954..9078
                     /locus_tag="ACF2SP_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HFD2358140.1"
                     /translation="MEQTSAQIVFEQKNATYQCCGSWSVLTLDGLPKKFSTSPLPDSQ
                     QIIINGEKISVFDSAGALALKKCIDELKKRNNQVELVNFSDSQHQMLELIESKQDILS
                     YQLPAVSKEGVLYQIGKESENKLHQVDGLIILIGDLSTRLFEAFAYWRRFHLPSIVSV
                     IDSAGLKALPIIGLLSFLIGVVLAYQMGLQLETYGANIFIAYLSGMAIFREFGPLITA
                     IIVAGRTSSSFTAQIGSMKINEEVDAILTMGLSPTELLVLPKVLGLLIVFPLLIFWAD
                     VFSIWGAMIMSKNMLNIGFEDFLTRLRDSVGLNQMMLGLYKAPAFSILIALVGCFQGF
                     RVESSADSIGSQTTKSVVQALFLIIIADAAFSIAYSWMGL"
     gene            9079..9816
                     /locus_tag="ACF2SP_000330"
     CDS             9079..9816
                     /locus_tag="ACF2SP_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358141.1"
                     /translation="MSEPVIEIKGMKNCLGGQWVHTDVNLTVERGEILAIIGGSGSGK
                     TTILRSLLMLLKPSAGSIKVFGEEIYNLDTEQAFRLRRRWGMLFQHSALFSAMTVLEN
                     VMFPMREFTTLDQEFMQELALLKITLVGLSKEAAVKYPSELSGGMQRRAAAARAIAMD
                     PELLFLDEPTTGLDPRGARLFDDLVVFLRDALNLTIVMVSHDIESLKRTTDRVAFVGD
                     GKILSVEPINELMKNKNPLIAEYFSKF"
     gene            9846..10769
                     /locus_tag="ACF2SP_000331"
     CDS             9846..10769
                     /locus_tag="ACF2SP_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HFD2358142.1"
                     /translation="MESKTNYTIVGLIVLILTAGLLSAGLWLSVGFNQKEYTSYTVYL
                     KESVSGLSVESPVKFNGVQVGYVKEIKLNKNDPRQVELLLNIEKSTPITTSTSATLIT
                     QGITGVTYVGLSAGSSELTPLRKMPGEPYPVIPSKPSLLNQLDAVLKEVAENVGAVSE
                     KAQLIFNEENADNVRKSLANIERITEIIADKGQTIDSSLNNLDVFVANMAKASKQFPQ
                     LIKDLKTGISKFKSLADNMSAAGKDVSKTMIAGKNTIDQISQQAIPPAVILLRRLNAI
                     SANLEKVSNEMRQNPSVIIRGTKAPKLGPGE"
     gene            10773..11375
                     /locus_tag="ACF2SP_000332"
     CDS             10773..11375
                     /locus_tag="ACF2SP_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HFD2358143.1"
                     /translation="MRVLSSVCFILGILTLMSCSPVKVPVTNEYQLTAYSTKQLTRKP
                     KPITIQVTVPEAVAGYQTEEMLYMKKPFKLEPFVKNAWTSPPADMLFPLLVQSLQSSG
                     YFYAVTSSPYSEEADYRLDTQLLKLEQNFIKKPSVLEFSAKIVLTHISDNQIIGSRIV
                     SLQIPCSQDTPYGGVIAANQATFRFTATATDFVVSHIKRD"
     gene            11473..12003
                     /gene="pal"
                     /locus_tag="ACF2SP_000333"
     CDS             11473..12003
                     /gene="pal"
                     /locus_tag="ACF2SP_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFD2358144.1"
                     /translation="MKAGSFYKLGLLVASAVLVAACSKTPGSADGGAAVGDGDATAQG
                     LGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARV
                     MIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEA
                     SHAQNRRVEFIYEATR"
     gene            12000..12968
                     /gene="ybgF"
                     /locus_tag="ACF2SP_000334"
     CDS             12000..12968
                     /gene="ybgF"
                     /locus_tag="ACF2SP_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HFD2358145.1"
                     /translation="MINCKKSIITLCFVLMLPFASWAEAPVIDDSENFAMIDRQEEYD
                     APLVNPKYDDPQIENAELDGSQNDNYSTDTSQSYDEPALVKEDRSTISDNAKLIDKIQ
                     QLQKEIQELRGQLEVQAHDLKLLQQQQVAFYKDLDSRLSNSSTSAKTVQNDKPATAIS
                     LGSNSPETLKVASPQIKAGSSNSKPQPVVAVSRANPADEQISYLAAYELVKNKRYDEA
                     IKSMQTFVQKYPRGGYTANAEYWLGELYLVKKDYSKAIEHFEIVLQQYPSSSKAAASL
                     LKSGYAYAEKGDKQEAKKRFQQVVKTYPDTPTAQLASSKLEAINAL"
     gene            12965..13618
                     /gene="queE"
                     /locus_tag="ACF2SP_000335"
     CDS             12965..13618
                     /gene="queE"
                     /locus_tag="ACF2SP_000335"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFD2358146.1"
                     /translation="MKRFNEQLRITEIFHSLQGESITVGLPTVFVRLTGCPLRCQYCD
                     TAYAFSGGEVVEIDDILNKVASYQCQHVCVTGGEPLAQPGCIPLLSKLCDAGYSVSLE
                     TSGARDIASVDQRVMIVMDLKTPDSREADKNLLSNLSFLKPTDQIKFVLCSRTDYEWA
                     CSMLSEYQLAERVQLLFSPSWNQLNPTDLANWIIQDKLPVRFQLQLHKILWNDAPGH"
     gene            13636..14583
                     /locus_tag="ACF2SP_000336"
     CDS             13636..14583
                     /locus_tag="ACF2SP_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate/mevalonate 3,5-bisphosphate
                     decarboxylase family protein"
                     /protein_id="HFD2358147.1"
                     /translation="MHWFAQAPANIALIKYMGKKDENSNLPDNSSLSYTLSNLLSSVK
                     LEKLPTKKDIWEPLTIPGAPEFNLSVEAQKRFIDHLVRLKEYFGYVGGFLIQSSNNFP
                     HSSGLASSASSFAALTKCASIALSELTQKPLPSIDEQAQLSRLGSGSSCRSFYAPWAL
                     WTGDKVSAIDLPYKDLLHQVIVISSQEKEIPSRVAHKLVKTSPFYETRSERAEANLKL
                     LLNAFENKDWTSIYQICWHEFLDMHQLFKTCEKPFSYITDNTLHILSVIEKFWNEKGD
                     GPVVTMDAGPNVHLLYRSDQTDLARQFKSDHLVGNYDVL"
     gene            14573..15451
                     /locus_tag="ACF2SP_000337"
     CDS             14573..15451
                     /locus_tag="ACF2SP_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFD2358148.1"
                     /translation="MFYDFETTTFGKWILAGEHAVVRGHEALVFPIKERQLTLKYNPS
                     SPSLSAEFQGINGSDMHLLFWSVLERGMHLLGRSLNELGGHFYLETNIPVGVGMGASA
                     ALCVAMSRWYCAQQMIQDNQCNEFAKQLEHLFHGKSSGLDIAGVASDTGVYFKSGLCT
                     PVKQTIYPYWYLSSCNQIGITSHCIQQVEYLWNKNPELAHQVDRQMVEAVQEAKAVLE
                     EGGSNALNRLAKAINKAADCFLQWGLVSESLQQHMNHLFADGAIAVKPTGSGGGGFVL
                     SLWDKIPSSHTELIPV"
     gene            complement(15464..15733)
                     /gene="ftsB"
                     /locus_tag="ACF2SP_000338"
     CDS             complement(15464..15733)
                     /gene="ftsB"
                     /locus_tag="ACF2SP_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFD2358149.1"
                     /translation="MRPIFIILIVALVALQHKLWLGDGNIIQWIKLEKKLEAHKSQND
                     KLAARNKALEADIKELKSGDQALEEQARYELGMIKQNEVYYQFVD"
     gene            complement(15955..17226)
                     /gene="eno"
                     /locus_tag="ACF2SP_000339"
     CDS             complement(15955..17226)
                     /gene="eno"
                     /locus_tag="ACF2SP_000339"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFD2358150.1"
                     /translation="MMHIHKIQAREILDSRGNPTIEADVILTTGIIGRASVPSGASTG
                     SREACELRDNDPKRYAGKGVQKAVKHVNNEINQALQGLSVEDQENLDRILCQLDNTEN
                     KSHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNV
                     DIQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNSQA
                     LDLLSEAIEKAGFRLGEDIVFALDVAASELFNEGFYHMSSENQKFDSHQLIEYYANLI
                     SSYPIVSIEDGLDEKDWSGWKQLTTHLGNKVQLVGDDLFVTNPKILREGIAQGIANAI
                     LIKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSL
                     CRTDRTAKYNQLLRINELASLPYAGKNILKR"
     gene            complement(17255..17857)
                     /locus_tag="ACF2SP_000340"
     CDS             complement(17255..17857)
                     /locus_tag="ACF2SP_000340"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFD2358151.1"
                     /translation="MSKFLQQKPIILASSSTIRHKLMKSLGLDFLVVPSNCNEEEIKT
                     RHNSDELVELGITLAKIKALDVSQHYPEHYIIAADQLCVADKRVFNKPLNHQTAVSHL
                     RELSGKEHQQIACLCIVKESKILWQYHETATLTLHHLSEKTIEAYLQAEKPYQSCGAY
                     QYEGLGKWLFKEVQGSEDTILGLPLMPLVNALVNLKVVGI"
     gene            complement(17850..18608)
                     /locus_tag="ACF2SP_000341"
     CDS             complement(17850..18608)
                     /locus_tag="ACF2SP_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFD2358152.1"
                     /translation="MLSATGLKIIFAVSIFIVILIAGWYPFKKRLKDDKHIDFPIGET
                     LATGVFLGAALLHMLPESNTLFKEMGYNYPFAFIITGVVFLIFLWFEHLGKELYHHHD
                     AEHPAFAILAWAMLSVHSLMLGAALGFTQYNSMIIMLFLAIITHKWAESLAIAIQLNK
                     SSMSTRKSMVFFFFFSLMTPLGIYFGWYFGHGVETNSLFDPILIAASAGTFLYLGTLH
                     GLERCVMVERCCNLSDFSFVIIGFLLMASVAIYV"
     gene            complement(18724..19866)
                     /locus_tag="ACF2SP_000342"
     CDS             complement(18724..19866)
                     /locus_tag="ACF2SP_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFD2358153.1"
                     /translation="MYPHDRYWQAKKVTLIGAFINALLGVVKMIGGFLYHSHALIADG
                     VHSISDLITDVMVVFASKYGSVSADDSHPYGHQRIETAATLLLSLLLILAGCGIAWDA
                     LDELLKSDHTMPNWLSIPIVCFSIIANEALFHYTRHVGKKISSQLITANAWHHRSDAA
                     SSIVVLIGLIGSLAGLVYLDAIAAVIVSLMIIKMGWSYGWNSVKELVDTAVDAELFSQ
                     IERVIQSIDGVQRIHQLRSRFMGSDVLIDVHILVSPKISVSEGHYIAQHVHHTLVKEI
                     DCVKDVIVHVDPEDDEISCPSLHLPNRAILQEKFFNDIRHDFPQILFWNIHYLNGKMS
                     LDIFCSNDFNQWKKLHNRVTSTLKSQIDIKEVRLFGLHEEMHHPSN"
     gene            complement(20379..22451)
                     /gene="recG"
                     /locus_tag="ACF2SP_000343"
     CDS             complement(20379..22451)
                     /gene="recG"
                     /locus_tag="ACF2SP_000343"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFD2358154.1"
                     /translation="MLSNSCEYLTGVGPTIAAKLAKCGIFTIQDLLFHLPYKYQDRTR
                     ITPIQDLRSNEWCVIAGHVCKTEIKYGKRMMLNCYVEDKTGVVKLRFFHFNKQQIQAL
                     NNSTMIRAFGEVRGFNNQLEMIHPEYQLIDQESDFHVEETLTPIYPSTQGLTQTRLRQ
                     LVKIALEQSEHELHQLEWMSEKQLQENNFYDLGEAIKLLHNPPPDISLSNLEAGEHPA
                     LKRLIFDELLAQQLSMQFARQSRSKLQAPAILFDNAIHKRFIESLPFSLTNAQQRVFK
                     EISVDLTQSKPMLRLLQGDVGAGKTIIAALAALQAISQGFQVAFMAPTDLLSEQHTNS
                     LSKWLEPIGINVLRLSGKMKATERKNALAALQDNSCQLIVGTHALFQEQVEFACLGLV
                     IIDEQHRFGVEQRLLLQQKGQLNQLIPHQLLMTATPIPRTLSMSHFAHLDISVIDELP
                     PGRMPITTAVLNQDKRELIIERLQAAIANGKQAYWVCTLIEESEKLQCIAATDTSKKL
                     QEQLSFARVGLIHGRMKALEKEATMAAFKQGEIDLLVATTVIEVGVDVPNASLMIIEN
                     AERLGLSQLHQLRGRVGRGNNQSHCLLLYQSPLSQQGAERLKIMRSTTDGFLISEKDL
                     ELRGSGEILGTRQTGFRQFKIANLQRDKTLFAILRPIAKQLVLEKTAIARQITQRWLG
                     NFEQFLQS"
     gene            22598..23656
                     /locus_tag="ACF2SP_000344"
     CDS             22598..23656
                     /locus_tag="ACF2SP_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD2358155.1"
                     /translation="MNRIITFTAGLILVWITGYLLIAGRGLLIPIVISIFVWHLLNTI
                     NSGVQKIPFIGTHLPSWIRMILSLFVVVCLVMVLINIITNNVSDVIAASPRYQENLLH
                     IFNSLDQRFHVKGIFDFDSLIQGLSIQSMLVNIYGVFTTITSSAVLISLYVVFLFVEQ
                     HYFTQKMDAFFPQAEHRKLVKNIISHITKDTQTYLGIKTLLSLLTATLSWFIMKWVGL
                     DFAEFWALLIFFLNYIPNIGAIVATAFPAALALIQFESWWPFLEVTSGIVAIQFFVGN
                     FLEPRLLGKSLNLSPLVILFALALWGAIWGVLGMFLSVPITVMMMIIFAHFDVTRPIA
                     ILLSQDGYIHKSYESLEN"
     gene            23760..25529
                     /gene="argS"
                     /locus_tag="ACF2SP_000345"
     CDS             23760..25529
                     /gene="argS"
                     /locus_tag="ACF2SP_000345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFD2358156.1"
                     /translation="MRSMKAIIEYLLKQALINLQQSGEMPIDLEVEIKVENAKDPSHG
                     DYATNLALVLAKPCRQAPKVLAERLVAVIPADPSVEKIEIAGAGFINFFMRSTARSLI
                     ISEILNKGKEFGRGNLGQSQKVLIEFVSANPTGPLHVGHGRGAAFGATLGNVLKAAGY
                     DVTLEYYVNDAGRQMNILAVSVWLRYLELAGEPIVFPTNGYKGQYVYEIAQEMWSEQG
                     NQFVHPWISVVENLPADEPEGGDKETYIDAIIARAQSLLGKDGFANFHQHALKTVLDD
                     IKDDLQAFGVRFDSWFSEQSLFEDGSIEKGIQALKDRGHTYEREGALWFRATDFGDEK
                     DRVLVRANGQTTYFASDVAYHWNKYDRGFDRVIDIFGADHHGYVTRIKTAVKALGHDE
                     SALDVILVQFAILYRGGDRVQMSTRSGSFVTLRELREEVGNDAARYFYVARKPEQHMD
                     FDLDLAKSESSDNPVYYIQYAHARICSVLRQLKERGLKWDKDMGLKNLDLLEQQHETT
                     LISLIARYPEVIQSAAASCEPHQLAYYLRELANGLHSYYNAIQLLCEQEQLRCARLCL
                     LESVRQVLNNGLAILGVSAPESM"
     gene            25529..26224
                     /locus_tag="ACF2SP_000346"
     CDS             25529..26224
                     /locus_tag="ACF2SP_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFD2358157.1"
                     /translation="MAKDYGNRRAARRNRGPHQFLVIIVTFLFGYITASFLDVQTISQ
                     WVNTQVLAQHDDEKAPTKVAQEHKQVPPKPKFEFYTLLANERGPASQASSQQNTDNHA
                     ADKTSTPIQKTANTVTAAVNNSVKSTITNPTPQTPVKVAEAKPTTMQQPNKGAYLVQV
                     ASFKARQDAEQMKGMLILKGFDVSVVPVTQAQGNWFRVVVGPYPNKALAQKAQMNLAK
                     TEHLNGMVRSVGG"
     gene            complement(26264..26602)
                     /gene="folB"
                     /locus_tag="ACF2SP_000347"
     CDS             complement(26264..26602)
                     /gene="folB"
                     /locus_tag="ACF2SP_000347"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFD2358158.1"
                     /translation="MDTLNITALSVETKIGVYGWEQRINQRLLIDISIPFDFKDCKDN
                     LSNTLDYADLCQTVTQFVESTSFQLIETVAEQIAQLIKQKYPVSKVSVAVTKPHAVKN
                     AAGIQVVVNR"
     gene            26842..27900
                     /gene="hemE"
                     /locus_tag="ACF2SP_000348"
     CDS             26842..27900
                     /gene="hemE"
                     /locus_tag="ACF2SP_000348"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFD2358159.1"
                     /translation="MFDLNQSLFLRALRRQPVERTPIWIMRQAGRYLPEYRKVREHAG
                     DFLNLCKNPELACEVTLQPLRRYALDAAILFSDILTIPDAMGLGLYFAEGEGPCFTNP
                     LQDTKAIHALKIPSIPESLSYVFDAARLIRQEMPKELPLIGFSGSPWTLACYMVEGGS
                     SRDFKRILNLIYTEKEAAHLLLNKLAVSVTAYLTEQIKAGVNAVMIFDTWGGVLTPQN
                     YKDFSLAYMHQIVQQLKNEYPDIPVILFTKNGGQWLEWMAETGCDALGVDWTCDLASA
                     RKRVGGKVALQGNLDPAVLLTAKNCIRREVGSVLASYGYGTGHIFNLGHGITPDVPPE
                     NVAIMIEAVHEISPQYHL"
     gene            complement(28198..29535)
                     /locus_tag="ACF2SP_000349"
     CDS             complement(28198..29535)
                     /locus_tag="ACF2SP_000349"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HFD2358160.1"
                     /translation="MQEWSPTTWQKYSYLQAASYADEEQLNKVVEQLSLLPPLVTSGE
                     IKHLKNEIAQAGRGNAFILQGGDCAESFNDCRSEVISNKLKIILQMSLILLYGLRKPI
                     IRIGRIAGQYAKPRSSDYETINGVTLPSYRGDIVNSPEFTATAREPNPKLLLQAYSCS
                     AMTLNFIRALLDGGFADLHHPQRWDLGFVEHSPQKNEYQHIVDSIEDALDFLNAIDGI
                     HSSSISKVDFYTSHEALHLHYEQALTRQLKDGKWYNLSTHLPWIGMRTAQTDSAHLEF
                     LRGVQNPIGIKIGPAATPEWLSEVLSVANPQKEEGRVLLYTRLGAKLIDRLLPPLIDT
                     VRKSKIPVTWSCDPMHGNTETTEDGTKTRHFDNILSELKQALEIHRSMGSYLGGVHFE
                     LTGDNVTECIGGARGLAPHDLKTAYHSLVDPRLNYEQSLEMAIQLSHQFRSEP"
     gene            29815..30414
                     /gene="grpE"
                     /locus_tag="ACF2SP_000350"
     CDS             29815..30414
                     /gene="grpE"
                     /locus_tag="ACF2SP_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFD2358161.1"
                     /translation="MSKQNKKDWKKFKDEHKEEHKVENEILEEEIDEKSQHQEPALGH
                     PSYTALEEQLTLAEQKAHENWEKSVRALAELDNVRRRMEREVANAHKYGVEKLISALL
                     PVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEA
                     MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK"
     gene            30559..32493
                     /gene="dnaK"
                     /locus_tag="ACF2SP_000351"
     CDS             30559..32493
                     /gene="dnaK"
                     /locus_tag="ACF2SP_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFD2358162.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGDKPKVIENSEGHRTTPSIVAFTD
                     DNEILVGQSAKRQSVTNPEKTLFAIKRLIGRRFDDPIVQKDIKMVPYKIMKADNGDAW
                     VRVKDQDKAPPQISAEVLRKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGMDKKRGDSVIAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDLALIEYLASEFKKDTGIDLHNDPLALQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADASGPKHLNIKLTRAKLESLVEKLVERTIEPCKTALKDAGLTVSQI
                     NEVILVGGQTRMPLVQKTVEEFFGKEPRKDVNPDEAVAVGAAIQAAVLSGEVKDILLL
                     DVTPLSLGIETMGGVMTKLIEKNTTIPTKATQVFSTADDNQTAVTVHVLQGEREQASA
                     NKSLGRFDLRDIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKAQSIVIKASSGLS
                     EEEVAAMVKDAQSHAEEDKKFKEMAELRNQADSLIHSCEKSMKDLADELSEDEKKGIE
                     TAISELKEAVQGTDKTRIEDKLKVLTDASAKMAERIYAKKSSEGQAAQGQTQSQESTK
                     PVEEGVVDAEFEEVKEEDKK"
     gene            32682..33821
                     /gene="dnaJ"
                     /locus_tag="ACF2SP_000352"
     CDS             32682..33821
                     /gene="dnaJ"
                     /locus_tag="ACF2SP_000352"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFD2358163.1"
                     /translation="MEQRDYYELLEVSRNASDAEIKKAYRRLAMKYHPDRNPGDTSAE
                     EKFKEIQKAYNILSDKQKRAAYDQFGHAGVDPSMGGGPGGFGGFGGFGDVFEDIFENI
                     FSGGRGHGRQSRGQRGADLQFNVQLTLEEAAIGKEVEITVPRHGTCTVCEGSGAKKGT
                     SPKTCETCQGMGQVRIQQGFFSIQQTCPTCHGEGKIISDPCASCHGQGRVRESKKINV
                     KIPAGVDNGDRVRLSGEGEAGVHGGGSGDLYVQISLKKHAIFERHENDLHCEVPISFA
                     TAALGGSIEVPTLEGRVTLKIPAETQTGKVFRLRSKGMKSVRGYGQGDLLCKVVVETP
                     VNLSREQKELLNKLQDSLENAKGTHSPKTSSWFAGVKKFFEDMKF"
     gene            33917..35029
                     /gene="carA"
                     /locus_tag="ACF2SP_000353"
     CDS             33917..35029
                     /gene="carA"
                     /locus_tag="ACF2SP_000353"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFD2358164.1"
                     /translation="MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEML
                     TDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDW
                     LSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVAKAKSFAGLQGADLAL
                     EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTS
                     AEEVLAMNPNGVFLSNGPGDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGI
                     TKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQG
                     IKHKTKPAFGFQGHPEASPGPHDIDILFNEFVNLMK"
     gene            35238..36386
                     /gene="metK"
                     /locus_tag="ACF2SP_000354"
     CDS             35238..36386
                     /gene="metK"
                     /locus_tag="ACF2SP_000354"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFD2358165.1"
                     /translation="MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACE
                     VLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSP
                     DIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
                     WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWL
                     NDKTRYFINPTGRFVIGGPLGDCGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSA
                     AYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTH
                     FDLTPQGIIDQHDLLRPIYRQTATYGHYGRENFPWERLDKVAELSKAL"
     gene            36405..37730
                     /gene="ahcY"
                     /locus_tag="ACF2SP_000355"
     CDS             36405..37730
                     /gene="ahcY"
                     /locus_tag="ACF2SP_000355"
                     /EC_number="3.13.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HFD2358166.1"
                     /translation="MELVEEGVQKINTLQDYKVADISLADWGRKEIAIAETEMPGLMA
                     LREEFSARKPLQGARIAGCLHMTIQTAVLIETLVALGAEVRWSSCNIFSTQDHAASAI
                     AAKGIPVFAWKGETEEEYWWCVEQTLSGPNGWTPNLLLDDGGDLTQVVHQKHPKLLTA
                     IKGVSEETTTGVARLYEMAKHGQLKIPAINVNNAVTKSKFDNLYGCRESLLDGLKRAT
                     DVMIAGKVALILGYGDVGKGCAQALRGQGATVLVAEIDPICALQAAMEGYRVVTLDDV
                     AEQVDIVVTATGNYHVVTHDHMKRMRNQAILCNIGHFDSEIDIQSLKQYQWENIKPQV
                     DHVIFPDGKRIIILAEGRLVNLGCATGHPSFVMSASFTNQVLAQIELFQNSSQYQNQV
                     YVLPKVLDEKVARLHLGRIGAKLTQLSNEQADYIGVDKNGPYKPDHYRY"
     gene            37854..38861
                     /locus_tag="ACF2SP_000356"
     CDS             37854..38861
                     /locus_tag="ACF2SP_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFD2358167.1"
                     /translation="MDLVIAANYLSTLEKCLEQFGINHFLEKIGFDLIRVHDPDAYLT
                     QDEFNQVILEAYKLSNCPELGLVFGQNLSIVNHGFLGYAAMSSPTFGTAIQTVLSYLN
                     TRTNLLSLELRQFNNENRAYIKLLPHTKEMVLSRFITELAVTHLIKMRNFLISCNDPL
                     LKIELNYDEPPYAKYYQTIFQTKIVFNTEATRVWFKAEELNYPIHFADDVSYQLAKRQ
                     LQELEKKMSLKEDMASKIKSILINQNLNHASMDEIASRLCMTSRTLRRHLQRLDVTYQ
                     ELLDEVREQKAKTFLLNNNISMTEISFLLGFQDTSNFSKAFKRWTGVTPTEYREKHEV
                     Q"
     gene            38993..40681
                     /locus_tag="ACF2SP_000357"
     CDS             38993..40681
                     /locus_tag="ACF2SP_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFD2358168.1"
                     /translation="MGLKSGILGLSLLSLACGSFAMTETETIRVIIKYKQQPATMASL
                     KSQVIKSVQIPVKEMKPMANGAYSLIFDTSNSHAIVKAKEEDVTAKVLERLRKNPNVL
                     YAVKDRIGHFKPLPDPVINQELSQLLSHESQWDEFERPAGIRLESKPGLRDGAWAYTT
                     GKSRKPIVVAVLDTGIALNDSLVNNLVKDNEGNVFGWNFAGNNNDLSDETRSYHGTHV
                     AGTIAGYGDVMNGVGEDLKILTVKIPAENGMFYESAVVNSIYWAVGGDVPGVPKNIHP
                     AKVLNMSFGVDLEPGKEIDYCDEALQEALFFARKKGAVVAVAAGNDNVWEHYNAPAVC
                     NGTIKVASTGPEGLRSYFSNYGPSITLAAPGGDKRYGTSGGILSTVNPGGGYNSSGFD
                     FYQGTSMASPHVAGVAGLILAVSNKALKPEQVEQILYVTTHEFGQSNDTNKSCVGKKP
                     CGHGILDADNAVQAALNAYDVLFSAPKMTDLATHECGENRLKPGKNIINDSTATWIQA
                     KSSCETGASYQNPRIEKAKDGKIYAYYGSASYELDQSAYKECRVVGYDGVGCYL"
     gene            complement(40835..41116)
                     /locus_tag="ACF2SP_000358"
     CDS             complement(40835..41116)
                     /locus_tag="ACF2SP_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358169.1"
                     /translation="MAETIDDITIAFNENGVETTRELDKQILSKGAWTTIMFKYQDWD
                     NTKNDYGPVKYSIRRYQKRNNQYWQKSKFNISSTEQAKKIVDILNNWLE"
     gene            complement(41130..41732)
                     /locus_tag="ACF2SP_000359"
     CDS             complement(41130..41732)
                     /locus_tag="ACF2SP_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HFD2358170.1"
                     /translation="MKSILLMSLLLLIMPFRLMSAQSLYCPQNHGYINIGMSMEQVIA
                     ACGQPLSKQESNQPILQKIPVQQLIYNNLGNSDGLYSGSLNVPEGTAFYGVWNIPTGS
                     SGVQLEVDILNNKVQDIRVNGGNTNAFSLCGNASIQIGDPATKVYGACGSPNLVNNTY
                     INQVVPTETKPQIWIYKPGPYQPAFSLTFVDGKLQSIGNN"
     gene            complement(41792..>41918)
                     /locus_tag="ACF2SP_000360"
     CDS             complement(41792..>41918)
                     /locus_tag="ACF2SP_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFD2358171.1"
                     /translation="LRLGRIIVPDISGFDFSPFIIMVILKIITLFLSASLPWKLL"
BASE COUNT        12620 a         7559 c         8881 g        12858 t
ORIGIN      
        1 aataaacaaa ccgatttaga taaaaaaata tgtttaggcg ttttctgtag ctatctacag
       61 aataagttga aaaggagtat tcgttgtgga aaataacttt aggctacaaa tctgtaagaa
      121 catcattaat tatctattag agtcaactaa ctatactcta aaagacattg ctgctttact
      181 aaattgctca ataagacaat tacgttctat ttatttcgat gagttaatgc caactaatct
      241 atattgcgaa cgggatcttg tgaggcttta tcatttaatt cttgaactca atactaataa
      301 atatgtgcac cagggttata aatggggttg cttatgaata cttttaatat tgaagaggct
      361 tcaaagtttc taggcgccca cagggaaact atccgacgaa tggctgcatc aggtgaacta
      421 cctggagtaa agatagggcg aggttggcgt tttatagaac aagatcttgc gatgtatatg
      481 cgaaacaagt actctacttg cgatgcatcg tggggtgtcg atataggaga taataaatgg
      541 cactcacgaa aaaaaatggg ttttggtgga tcgacgtcac cttcggtgac aagcgtatac
      601 gcagaagcac taggactaaa gtaaaacaac aagcgcaaca attccatgat aattttctac
      661 aagatctatg gagtaacaag cataccaaat taccccaaca gaaatcgtgg gtagatgctg
      721 ttgtgcgatg gttagaggaa tcagcgcata aacgaagttt agttacggat aaatttcatt
      781 tggcctttct ggacccttat cttggccaga aaacgttaga tgaaattaac gccgaattaa
      841 ttgaatttat tgcgcaagaa aaagagcgga ctaatgttac tccaacgacg gtcaaccgaa
      901 tgttggaaat tatacgtgct attttaaaca aagctcataa ggaatggcag tggataaaca
      961 ccatgccatt attcagaatg agaaaagttg aaaataaacg tatacgttgg ttgagtaggg
     1021 gacaggctaa taaattgatt caggagctac caaatcattt gaaagatatg gcagctttta
     1081 ctttggcaac tggtttacgc caatctaatg ttactcaatt gcagtggagt gaagtggatt
     1141 taattagaaa acatgcactc attcaccctg atcaatctaa aacaaagaag gctatccctg
     1201 ttcctttaaa ctctcaggca attgtcatta tagaaaaaca agtgggctta catcccacat
     1261 ttgtatttac ctattttggt aagcctgtta cgcgatgtaa taatcatgct tggcaaaaag
     1321 cgttgattcg ggcaggaatc aaagattttc gctggcatga cttgcgtcac acttgggcaa
     1381 gttggcatgt gcaaaatggt acctcattgc aagaattaca gcaattaggt ggatggtcag
     1441 aatatgaaat ggtgttacgt tacgcgcatc tttcaagtga ccatttaaag gtggcagcgg
     1501 aaagaattac aaaaaattct ggcacgtttt cgtcactgta acttggtttt tacggcacgt
     1561 ttcgatccag gcaaccgtta actacctgat tttaaagggt ttaaatggtg cgctcggagg
     1621 gactcgaacc cccgacacct tggttcgaag ccaagtactc tatccagctg agctacgagc
     1681 gcaatggggt gtggatgatc attttatttc tggtctgttt atgcaggagg gagaccagaa
     1741 tggtgggccg tataggattc gaacctatga cacagaggtt aaaagcctcc tgctctacca
     1801 cctgagctaa cggccctttc agggtataac cagcaaaata caatagttga tggtataggt
     1861 ttattaaaaa ctggtgggcc gtataggatt cgaacctatg acacagaggt taaaagcctc
     1921 ctgctctacc acctgagcta acggccctaa agaggctgaa atcataccgg attagagcag
     1981 aatttcaagt ctttttagag attatttttt gtttaacaaa caagatgtgt tatttatatg
     2041 cttttttcaa acaaggaatt attattcctg acagttagat tatcttctgt agctaaaatt
     2101 aaccagtttt gatcttgaac tttatctttg agagattgta agtctctttt atcggtaaga
     2161 attaacaaag tatcagctcc taaagcacca cagcctttta ttgccaaggt ttccggataa
     2221 gattttatag attgaataag aggcagacta tgttgactca ccagatttaa ttctgtcaat
     2281 ttttgagcat ataagttaat acattggatt aaatggtggc tatgagccaa atcaaaagct
     2341 tcttttgctt tgataaccaa agaggcgagg taatctattt gagatggcag ggtgacttct
     2401 tgaagatgat gatgtgtcgc caatttgttg ccggtgtgaa tgattaagaa ggataaatct
     2461 ttgaatggcc aggagaacga attaacctga tttttttgtt tatcaatgta aacacaacct
     2521 tgctgtgact gagcaatcac atcataacca ctgggtctta accccaaccc cgaccaggac
     2581 gattggtaat aggcctcaag catatggttt aatcgagggg taatctcttg cagtgaacaa
     2641 gttgccaaat aacaggctaa aaattgcgcg ctggatgcgc ccaaaccgcc ttggccatga
     2701 taagggtctt gccaggatag accaaaggga tagcattgat ttttttgcca ccataatcca
     2761 gcaggtgact ccggatgaat tccttctaat tttgcatttt cagttaatgt aagaataaag
     2821 caagggcttg tcgttaatag gatagctggt tctcccgcaa tggcagcgta ttctcctaat
     2881 aaaaatgttt tagcgggaat ttgccatttc atactgcaac aggagcttgt cggatttcag
     2941 ctaataggtc ataagcgtta ttcaagctga ctcgtttgtt catgttaagc cattcttgca
     3001 atttttcttt gagcacgggc atttcatttt cattagcccc tgcaactaat agcaaattat
     3061 caatatgcaa tttcatatgg ccttgaatga tcccgtcagt gcataaagct ttgagtgcgc
     3121 caagattttg cactagtcca actgctgcga tcacctggga gagttgattg gcagaagtga
     3181 tatccatcat tcgtaaacac attttagccg tggggtgtaa agaggttacg cctccaacag
     3241 tacctactat gataggagca gttaactctc ctgtgagtat ttcattttga taccgccagc
     3301 gagtaatcgc tttgtattgt cccgaacgtg ccgcataggc gtgtattcca gcttccacag
     3361 ctcgccaatc attccctgta gcaattaaaa ccggatcaat tccattcata acccctttat
     3421 tatgggtagc agcgcgatag ggatctattt ctgcaaagag tgaggcctct tgcaattttt
     3481 gaccgaggat ggggtcaata ttacgaatga tgacttgagc ggtcgttaat ttttgatcgt
     3541 ttaaattgga taaaatacac atggtgactt cttcaccagt tatttgttct atgggttgtt
     3601 ttaaatattc aagtacttga ttgatgatat tggcacccat agcatcgcag ctgttcatcg
     3661 tcaaatgaat tactgccata tcttgcccat cttctctctt taaatgacgt acttgcaaat
     3721 cagtaacacc gccgcctcgt ttgaccatat tggcggcaac gtctttattc gctatttcta
     3781 taaaatattg acgattttta ttaaagaggt cagagaatct ttgaaagtcc tttacttttg
     3841 ccagttgaat ttgcccaaga atacattctc cgtgaaccca ggtattgatt tcaccatgct
     3901 gcctaatcca tttcgctgat ttggaaagag cagcaataat agaggtttcc tccactgcca
     3961 aaggtattac ataatcacgt ccatcaatat taaaattagt agccacaccc aatggaagct
     4021 gaaaatatcc aatcacattt tcaatcaatt tgtcggccag atttaaatct ttaatgccgc
     4081 cttgtttaag aaatgttatg tcttcatgag tcaaggcacc taaagcgcat aacctttgaa
     4141 agcgctcttc acgtgataac tttgagaatc cgcgaaataa ttcaccggca ttagcagcta
     4201 tggacatgcc ttctccttta aatcatcaag aactcggctg ccagtacaaa acatagcagt
     4261 tttcaattca tattcaattg tattcatttg ggtcagtact tgcccggttg agtccaaagc
     4321 cgcttcaagc atgggtttcg cgaagccgac tgtggtagcg cccagcgcaa acagttttgc
     4381 agcatccaaa ccatttctta cgccgccaga accccaagtt tcgaaagagg gattaagact
     4441 aatggcgtta cgaatgcttt gtaaagtatc tatgccccaa tttcgaaaag tatcagcagt
     4501 tcggtgtctt atcggatcct tatttgcccg atgtccttca attcgccccc aatgcgtccc
     4561 ccccacacca ctgacgtcaa cagcggcaac acctatatta tttaagcgga gcagcgtatt
     4621 ttttgaaaaa ccacaaccag tttcttttat tataactgga gaagcaatct ttttgactaa
     4681 tgcttccaat gctgcccagc atccttgaaa attagttgtt ccttcaggtt gaatgcactc
     4741 ctgtaatgga ttgcaatgaa taatcaatgc ttcagcttgc aatgtatcaa ttaagcgctc
     4801 gattgcagaa attggagtgt caattaactg ggcgattccc agattactaa acaggctgac
     4861 catggggaaa tcacggcgga gtggcgccca ctcaaatgcg gcttgtttat cggttaattc
     4921 tctgcgttgt gagccaacgc ccattgccca ttttgttttt gagcacgctt ccattaatcg
     4981 atagttaatt tctagagcat tggaatggcc tgctgtcatt gaactgataa taaatggttt
     5041 ttcaaccggt tttttaaatc ggatgctttg tatggaaatg tctttaaaat caaggtcagg
     5101 taaggcttca tgcaccaaag aaaaatgatc aaagggattt aattcgctgc tttgattggc
     5161 tggcattaag gccagttcta tgtgatcgcg cttacgctgt tcaaattggc tgtattcgtt
     5221 ttgcatgcct gaatttactt ttgaaaaata gcgtcaattt tagcataaaa tcatgaaata
     5281 gaccattgca tttctattcg acatagtgat ttatttggta tcattgattt aaccatgagt
     5341 taactaattt tttcgtttag ttggcttttt ggtaacaaat taaggtaatt atcatgaaat
     5401 ctaaacttga ataccatttc tctgcaaacg cggatggcga tgaatcctgt attgaagatg
     5461 ttgatggaaa tgaatttact ccaaatggtt caccatcaca tggatggact gcgtggacta
     5521 gattttttgg ccatcaaaac cagtctccta aatatgttgt taaacaacct agaccagata
     5581 aagtccgttc ctcttcagat cttagagata aaaaacttaa taatgaatgg aacagaacac
     5641 gctatcagca tgaactggat atgtggaatt tggtttatcc agataataaa gcctatttgt
     5701 ttacagataa cggaattcga ttggttctcc cttatttacc cggtaaaact ctattttctt
     5761 ttactagtag ggaagaagat cagttaactt ggtgtcaact aaactatgca gtattttgtg
     5821 agtttaagcg ctttattgaa cttggattga agcattgcga tgtaaaatat gacaattttt
     5881 tgatagaaag aaagcaagat ggtaccttta aagcctacat aattgatctc gattcagtgg
     5941 gtaaactaaa gcctaatgat caggattatg attggaatat tgtcctggca tctctattcg
     6001 acgataccgg actaaaaagt ccggatgttt gcccacccgg ctatattgct ttgaaatcaa
     6061 tcgatgttcc agcagaaaat gagaatgaag cacgatcaaa acttgataaa ataaaaaaag
     6121 aactatcgca aaaaaaacct accttggtaa gaatcaagaa ttgccagcaa aataagttgg
     6181 attattattt tgtttatgga tgtttgtata atgggcaatg gggctttact aaacttgatg
     6241 agaagatcat tagttcggtt aatctatcta atttttctgg tgctttagtt gattgtagag
     6301 ctgcttctgt tattggtaat catattaaat taaaacaagg ccacattagt tacagacttg
     6361 acctttatat caactgtctg aatgagaaaa tagctgaact cactctactg aaagctcacc
     6421 taaaaagctg aatatcccat tttaaatcag atccagtttc tatcatgacg ggatcaaagc
     6481 gtgctgctaa gctgtaagta tagtatcttt gtcttggtaa tgccttctta ggctatatca
     6541 atatagctat attctataca taggccatca gcgtcttatc gtctaccagc gataatagta
     6601 ataaggtcta tagtagggtc tgtagtaagg tctgtaatat ggataacccc agcgatagta
     6661 atacggtcta tagtagtatc cgcgatagta atatgggccc caatacctat agcgtaccca
     6721 gtaagccatt tgagtcggat tggctttatt aattatcgga ttaatatctc tgttaactgc
     6781 tttggcattt gcaggggtga ctaaaatcaa agctccaatc agaagggtaa aagctagcat
     6841 cagttttatt aaagaggtcc ctttaaagat aaaacgtccc attttttact ccttaaagcg
     6901 tactttattt aagtatagac tacacgggat tatttggtta atatttgcgc atgatcatta
     6961 ctgggcatga gtttaaattt tattgaggca gtaagtggtt tattaggatt aatgattaaa
     7021 aaaatgcccg agccttttgg ccgcagctcg ttcttcaagg ttctctagct tattgcgttc
     7081 caaggttgtt ttgaatagtc gctgtgattc gggatgaaag ttcatattgc gaataacgtg
     7141 ttctggttta agcaaggtat caggtaattg ataactcaat tgattctttt tctgtaactg
     7201 ttgataaaga gagttgctta agcccaagcc atgttgtagc gcctggtaga aacgtgatac
     7261 gggcccttgt aaagcggcaa tatggtcata gattatgcct ggttgtggag ctccaccaat
     7321 gttttcaaga accagcttta actcctcgag tttcgccata aataattttt gtttattcaa
     7381 aaccttaacc aaggtatcta ttggtttgaa ttgcttggtc tgttcaagtg atttcctttt
     7441 gtcatgagat tctttaagct gtttttgagc aataacacgt aataacgact ctaccaaatg
     7501 attaagcata gccagaattt gttttaattc gtctgttccc aaagcaaaag ctaatcgctc
     7561 cagattgaat tcaaaagaat gcttggcttc tatccctaat aattgaagca ggtattgttg
     7621 taaatgacct aactggctta aggcattttg tgtatccagt ctgtattgca aaaccagaat
     7681 aagggatttg agtgagtcca attcatccaa gtcggattct tcctgtagtc ctttttcctg
     7741 ttgtaaagac tccagcagct gattagattg agtgagttct gcttctgctt gtttgatgag
     7801 gttagattgg gtttcaatta acttttcatt catgaagata acctggatat aaaagagcac
     7861 tacctatatt attatgatac catgaatcgt aagtttttgc ggtcttgtgg tattttgtat
     7921 tattctagga aaattcaata aaaagaaata aatatggaac agactagcgc acagattgtt
     7981 ttcgagcaaa agaatgcaac ataccagtgt tgtggttcct ggtctgtttt gacccttgat
     8041 ggattaccta aaaaatttag cacaagccca ttacctgatt cacaacaaat aatcataaac
     8101 ggtgaaaaaa tcagtgtttt tgatagtgcc ggagctctgg cactaaagaa atgcatagat
     8161 gagcttaaaa aaagaaataa ccaggttgaa ttagtcaatt tttccgatag ccaacatcaa
     8221 atgctggagc tcattgagtc caaacaagat attctttcct atcaattacc tgctgtttcc
     8281 aaagaaggtg ttctttatca gattggtaaa gaatcagaaa ataaattgca ccaggttgat
     8341 ggtttgatta ttttgatagg agatttaagc acaaggcttt tcgaagcgtt tgcatattgg
     8401 cggcgatttc atttgcccag cattgtttct gttatagatt ctgccgggct aaaagcgtta
     8461 cctatcattg gattattgtc ttttttgata ggggtcgttt tagcttatca aatgggtttg
     8521 caattagaaa cttatggtgc caatattttt attgcctatt tatctggcat ggcaattttt
     8581 cgggaatttg gacctttaat tacagcaatc attgtagcgg ggcgcaccag ctcatcattc
     8641 acagctcaaa ttggcagcat gaagattaat gaagaggtag atgccatctt gaccatgggg
     8701 ctttctccca ctgaattatt agttttaccc aaagtcttgg gtttattaat agtgtttcct
     8761 ttattaattt tttgggcaga tgtctttagt atttgggggg caatgatcat gtccaaaaat
     8821 atgttaaaca ttggtttcga agatttttta actcgcttga gagattccgt aggacttaac
     8881 caaatgatgc tgggtttgta taaagcccct gccttctcga tattaattgc tttagtgggt
     8941 tgttttcagg ggtttagagt agaatcaagt gctgatagta taggaagcca aacaaccaaa
     9001 agtgttgtgc aagctttatt tttaattatt attgctgacg ctgctttctc catagcttat
     9061 agctggatgg gtttgtaaat gagtgaacca gttattgaaa tcaagggaat gaaaaattgt
     9121 cttggcggtc aatgggtgca caccgatgtt aatctcaccg tagaaagggg agaaatcctg
     9181 gctattatag gaggctctgg aagtggtaaa acgaccatat tacgcagttt gctgatgtta
     9241 ttgaaacctt ctgcgggtag tattaaagta ttcggtgaag aaatttataa tcttgatacg
     9301 gagcaagctt tcaggcttag gcgccgatgg ggcatgttat ttcagcacag tgctttattt
     9361 tcagctatga ccgttctgga gaatgtgatg tttccaatga gggaattcac tactttagat
     9421 caggagttta tgcaagaact ggcactttta aaaataactt tagtaggctt atctaaggaa
     9481 gcagctgtga aatatccttc cgagttaagt gggggaatgc aaagaagagc tgccgcggca
     9541 agagccattg caatggatcc agagctatta tttctggacg aacctacaac aggattggat
     9601 cctaggggag ccaggctttt tgatgatttg gttgtttttt taagagatgc gttaaattta
     9661 accatagtca tggttagtca tgatatagag tctttaaaaa gaacaacaga tcgtgttgca
     9721 tttgtaggtg atgggaaaat tctcagtgtt gagcccatta atgaattgat gaaaaataaa
     9781 aacccgctaa tcgctgaata ctttagcaaa ttctaagaaa cactataaca gaggggacaa
     9841 cttacttgga atctaaaacc aactacacca tagtaggcct catcgtactt attctgacag
     9901 ctggcctatt gtctgcgggt ttatggctct ctgtcgggtt taaccagaaa gagtatactt
     9961 cttatactgt ttatctcaag gagtctgttt ctggtctcag tgtcgaatcg cctgttaaat
    10021 tcaatggggt gcaggttggt tatgtcaagg aaattaaact aaataaaaat gatcccaggc
    10081 aagtggaatt attgttaaat atcgaaaaga gtacgccaat aacaaccagt acttccgcaa
    10141 ccttaattac acagggtatt acaggagtta cttatgttgg gttatctgct ggatcttccg
    10201 agctcactcc tttacgaaaa atgccggggg agccctatcc tgtaattcct tccaaaccat
    10261 ctcttctgaa tcagttggat gctgttttaa aggaggttgc tgaaaatgta ggtgcggtga
    10321 gtgaaaaggc tcaacttatc tttaatgaag aaaacgctga taatgtaaga aaatcattgg
    10381 ctaatataga gcgtattaca gaaataatcg ctgataaagg tcaaacaatt gatagttctt
    10441 taaataatct tgacgtgttt gtagccaata tggcgaaggc aagcaaacaa tttcctcagt
    10501 tgataaaaga tttaaaaaca ggtatttcaa aattcaaatc tttagctgac aatatgagtg
    10561 ctgcgggtaa ggatgtttca aaaacgatga tagcgggcaa gaatactatc gatcaaattt
    10621 cccaacaggc tatacctcca gcagtaattt tattgcgacg cttgaatgcc atttcagcga
    10681 accttgaaaa ggtcagcaat gaaatgaggc aaaacccttc tgtgattatt cgaggtacga
    10741 aagcacctaa acttgggcca ggggaataaa atatgagagt tctcagtagt gtttgtttta
    10801 tactaggaat attaacactg atgagttgtt cacctgttaa agtcccagtg accaacgaat
    10861 atcaattaac agcatacagt accaaacaat tgaccagaaa gcccaagccc ataacaatac
    10921 aagttacagt gcctgaagcg gtagcaggat atcagaccga agaaatgctt tacatgaaga
    10981 aaccgtttaa attggaacca tttgtaaaaa acgcctggac ctcacctccc gcggatatgc
    11041 tttttccttt attagtacaa agcctgcaga gttcagggta tttttatgcc gtcacatcga
    11101 gtccctatag tgaagaagca gattatcgat tggatactca actattgaaa ttagagcaaa
    11161 attttatcaa gaaacctagt gttcttgaat tttctgcaaa aatagtacta acccatatta
    11221 gtgataatca aattataggt tcgcgaattg ttagtttaca aataccttgt tcacaagata
    11281 ctccttatgg cggggttatt gcggcgaatc aggctacatt tcgatttact gcaacggcaa
    11341 cagactttgt tgtatcgcat attaaacgtg attaggaata gccaaaaaat aaaaagaatt
    11401 gtacaatggc tctccatgat tatcatgtgt agtttctaaa aaataataag aagagtttaa
    11461 ggagattgtg gaatgaaagc cggatcgttt tataaactgg gactgcttgt agcaagcgct
    11521 gtattggttg cagcctgttc taaaacccca ggcagtgcgg atggtggggc tgcggtaggt
    11581 gacggtgatg ctactgccca aggtttgggg caaatgactc actttgctgg acaagagcct
    11641 ggtgagtcct atacaacgca ggcaccgcat aatcaattgt atttgtttgc atatgatgac
    11701 agcactttgg catcaaaata tttaccttca gttaacgcgc aggcagaata tttaaagacc
    11761 cacccaggtg ctcgagtgat gatagctgga catactgatg aacgtggtag tcgtgaatat
    11821 aacgttgctc ttggcgaacg ccgtgctgat actgtagctg aaattctgcg tatggcaggt
    11881 gttagcagac aacaaattcg agtagttagc tacggcaaag agcgcccggc taattatggc
    11941 catgatgaag cgtcgcatgc gcaaaatcga cgcgtagagt ttatttatga ggcaacaaga
    12001 tgattaattg caaaaaatcc attatcaccc tatgttttgt actgatgctt cctttcgcct
    12061 catgggcaga agcgccagta atagatgata gtgaaaattt tgcaatgatt gacaggcaag
    12121 aggagtatga tgctcctctt gtcaatccca agtacgatga tcctcaaatt gagaatgctg
    12181 agttggatgg gtctcaaaat gataactact caaccgatac ctctcaatcc tatgatgaac
    12241 ccgcacttgt caaagaagac cgcagtacta tcagtgacaa tgcaaagtta attgataaaa
    12301 ttcagcagct tcaaaaggaa atacaagagt tacgtggcca gcttgaagta caggcacacg
    12361 atttgaaatt attacagcag cagcaagttg ctttctacaa ggatctggat tctcgattgt
    12421 ccaattcttc aacttcagca aaaactgttc aaaatgataa accagcaaca gctatttcat
    12481 tgggttctaa ttcgccagag actttaaaag tggcttctcc tcaaatcaaa gcaggatcat
    12541 ctaatagtaa acctcagcct gttgttgctg tttccagggc taatcctgct gatgaacaga
    12601 taagctacct ggctgcatac gagttagtta aaaataaacg ctatgatgaa gcgattaagt
    12661 ctatgcagac ttttgtgcaa aaatatccca gaggtggtta caccgcgaat gcagagtact
    12721 ggctaggtga attatatctt gttaaaaaag actattctaa agcaatagaa cattttgaga
    12781 ttgttttaca gcaatatcct tcatccagca aggctgcagc cagtttgtta aaatctggtt
    12841 atgcttatgc cgagaaaggt gacaagcaag aagctaaaaa gcgttttcaa caagttgtta
    12901 aaacatatcc tgatacacca acagcacaat tggccagctc taaattggaa gcaattaatg
    12961 ctttatgaaa cgatttaatg aacaattaag aattacggag atttttcatt cattgcaagg
    13021 agaatcaata actgtaggct tgcctactgt atttgttcgc ctgacaggct gtccattgcg
    13081 atgtcagtat tgtgatacgg cttatgcttt ttcaggtggt gaagttgttg aaatagatga
    13141 tattttgaat aaagtagctt catatcaatg tcagcatgtt tgtgttactg gtggggaacc
    13201 acttgctcaa cctggctgta ttcctttatt aagcaaactt tgtgatgcag ggtactctgt
    13261 ctctttggaa accagcggcg ccagagatat agcctctgtg gatcaacgag ttatgatcgt
    13321 tatggactta aaaactcctg attccaggga agcagataaa aacttgctgt ccaatttgag
    13381 ttttctgaag cccactgatc agattaagtt tgtactatgt agccgaactg attatgaatg
    13441 ggcatgtagt atgctgagtg aatatcagtt ggctgaacgg gtacaactct tattttctcc
    13501 gagttggaat cagctaaatc ctactgatct tgcaaattgg ataattcagg ataaattacc
    13561 agttcgtttt caactacaat tacataaaat tttatggaat gatgcacctg gtcattgacc
    13621 tcaaggagac taactatgca ttggtttgct caggcaccag ctaatattgc tttgatcaaa
    13681 tatatgggta aaaaggatga aaattctaat ttacccgata attcatctct atcttacacc
    13741 ttgagtaatt tattaagttc cgtgaaatta gagaagttac cgactaaaaa agacatttgg
    13801 gagccactta ctattcctgg agcaccagaa tttaacctgt cagttgaagc gcaaaaacgg
    13861 tttattgatc atttggtacg attgaaagag tattttggat atgtcggtgg gtttttaatt
    13921 caatccagta ataatttccc tcatagcagt ggtttagcga gctctgcctc cagctttgct
    13981 gccttgacaa agtgtgcatc aattgcatta agtgaactca ctcaaaaacc tttgccttct
    14041 attgatgagc aagcacaact gagtcgattg ggttcaggct catcatgccg ttctttttat
    14101 gcaccatggg cgttatggac gggcgataaa gtctctgcaa tcgatttgcc ttataaagat
    14161 ctattacacc aagtcatagt aattagcagt caggaaaaag aaataccatc aagggtagcg
    14221 cataagctgg ttaaaacaag tcctttttat gaaacacgaa gcgaaagggc cgaggcaaat
    14281 ttgaagttgt tactgaacgc ttttgaaaac aaagattgga cgagtatata ccaaatttgc
    14341 tggcatgaat ttctcgacat gcatcaatta tttaaaacct gtgaaaaacc attttcttac
    14401 attacagata atactttgca tatattgagc gttattgaaa aattttggaa cgagaaaggt
    14461 gacggccccg tagttactat ggatgctggc cctaatgttc atttgcttta tagatcagat
    14521 caaacagatc tcgcacgaca atttaaatcg gatcatttgg taggaaacta tgatgtttta
    14581 tgattttgaa accaccactt tcggcaagtg gattttagca ggtgaacacg cggtagtaag
    14641 gggacatgag gctttagtat ttcctattaa agaaaggcaa ttaactttga aatataaccc
    14701 ttcctcaccg tccttgagcg cagaatttca aggcattaat ggttctgata tgcatttatt
    14761 gttttggagc gtattagagc gtggcatgca tttgttggga cgttcactga atgaattagg
    14821 aggtcatttt tatttggaaa ccaatattcc tgttggggtt ggcatgggtg cttcggcagc
    14881 gctttgtgtt gccatgagtc ggtggtactg tgcgcagcaa atgatacaag ataatcaatg
    14941 taatgaattt gccaaacaat tagaacatct gtttcatggt aaaagtagtg gcttggatat
    15001 agctggtgta gcatcagata ctggtgtata ttttaaatcc gggctttgta ctccagtaaa
    15061 gcaaaccatt tatccctatt ggtatttatc ctcctgcaat caaatcggca ttacttctca
    15121 ttgtatacag caagttgaat atttatggaa taaaaatcca gaacttgctc accaagtgga
    15181 taggcaaatg gtagaggctg ttcaggaagc aaaagctgtt ttggaagagg gggggagtaa
    15241 tgctttaaac cgtttggcaa aagcgataaa taaagccgca gattgttttt tacaatgggg
    15301 tttggttagt gagagcttgc aacaacatat gaatcatttg tttgctgatg gtgcaattgc
    15361 tgttaagcca accggttcag gtggtggagg gtttgtatta agcctttggg ataaaattcc
    15421 ctcatctcat acagagttga ttccggtcta gctcctttca agcctaatcg acaaattgat
    15481 aatatacttc attttgctta atcatgccga gctcatatcg cgcttgttct tcaagagcct
    15541 gatcaccact ttttaactct ttgatatctg cttccaacgc cttatttctg gcagctaatt
    15601 tatcgttttg acttttatga gcctccaatt ttttttcgag tttaatccat tggatgatat
    15661 ttccatcacc tagccatagc ttatgttgta aagcaactaa agcaacaatt aagataatga
    15721 atatggggcg catagaattt tctattgaag aatgtatcta attactattg tagtatctta
    15781 ttaccacaag gggcaatatg tgttcagggt atatagtatt aaatttttta tcgaactgat
    15841 tttaaattgt attttattta tattcccttt gatttaaatt agagctgatg ctgagtttta
    15901 ttcagcactc aacaccaccc caacttaaac attaataatg ctgaaagctt gaactcatct
    15961 ctttagaatg ttttttccag catagggtaa agacgccaat tcattgattc ttagtagttg
    16021 attatatttc gcagtcctgt ctgttcgaca taaagatccg gtttttattt ggccacaacc
    16081 actggccact gcaagatcag caatgaaggt atcttctgtt tctcctgaac gatgggacat
    16141 gacacatcgg tatccattgt cgtatgcgag ttttatagct tgcctggttt cgcttaaagt
    16201 acctatttga ttgactttaa ttaatatggc attagcaatt ccctgagcaa ttccttctcg
    16261 cagaattttg ggattagtta caaataaatc atctccgacg agctgaactt tatttcccaa
    16321 atgggtggtt aattgcttcc atcctgacca gtctttttcg tccagtccat cttcaatact
    16381 aactattgga taactggaaa taagatttgc atagtattct atgagttgat gagaatcaaa
    16441 tttttgattt tcagaagaca tatgataaaa gccctcatta aaaagctcag aagccgcaac
    16501 atccagggca aatacaatgt cttcccctaa tcgaaagcct gccttttcaa tcgcctcgct
    16561 aagaagatcc agcgcttgac tattagattg aatattaggt gcaaaaccac cttcatcacc
    16621 aacagcggta tttaatcctt gcttttttaa cacagatttc agtacatgaa aaatttcagt
    16681 tcccatttgc aatgcaactg gaaaatcagg agcgccgatt ggcatgatca taaattcttg
    16741 aatatccaca ttattatcag catgagcgcc tccattcaga atattcatca taggaacagg
    16801 catggtcatc atatcaccct gattaagggt catatacaat ggctgattaa gagacaatgc
    16861 tcttgctctg gcacatgcca gagaagtagc caggatagca ttagctccca gatgggattt
    16921 gttttctgta ttatccaatt ggcataaaat gcgatccaag ttctcctgat cctcaaccga
    16981 caatccttgt aatgcctgat ttatctcgtt attaacgtgt ttaactgctt tttgtacacc
    17041 cttgccagcg tatcgttttg gatcattgtc tctaagttca catgcttccc tgcttcctgt
    17101 ggaagcacct gagggaacgc tggctcttcc aataatacca gttgtgagaa taacgtctgc
    17161 ctcaatagtt ggattaccgc gagaatctaa tatttcacgt gcctgaattt tatgtatgtg
    17221 catgatcttt cctaatcttt ttatatttgt taacttaaat acctaccacc ttaaggttta
    17281 caagcgcatt aactaatggc attagtggca aaccaagaat agtatcctcg cttccttgta
    17341 cttccttaaa taaccacttg cctaggcctt catattgata agcaccacaa ctctgataag
    17401 gtttttccgc ttgtaaatag gcttcaattg ttttttcact tagatgatgc agtgtcagtg
    17461 tagctgtttc atgatattgc caaagtattt tgctctcttt aacaatacac aggcaagcaa
    17521 tttgttgatg ttccttcccg cttaattctc tcaaatgact tacagctgtt tgatgattca
    17581 gtggtttatt aaatactctt ttatcagcga cgcataattg atctgctgca atgatatagt
    17641 gctctggata atgttggctt acatctaacg ctttaatttt agccagggta atccctaact
    17701 caactagttc gtcggagtta tgccttgttt taatttcctc ttcgttacaa ttcgatggta
    17761 caaccaggaa atccaagcca agtgatttca tgagtttatg tcttatggtc gaagaggagg
    17821 cgagaataat cggtttttgt tgtaaaaatt tagacataga tggccaccga tgccatgagt
    17881 aaaaagccta tgattacaaa gctgaaatca cttaaattac agcaacgctc taccatgaca
    17941 cagcgctcca gtccatgtaa agttcccaaa tagagaaatg taccggcaga tgctgcgatt
    18001 aatatagggt cgaataaaga gttagtttct actccatgac caaaatacca accaaaataa
    18061 atgcctaaag gtgtcataag gctaaaaaag aaaaaaaata ccatactttt tcgagtgctc
    18121 atcgagcttt tatttaattg aattgctata gccaaacttt ccgcccactt atgagtgatg
    18181 atcgctaaaa atagcataat gatcatggaa ttgtattggg taaaacctaa agcagctccc
    18241 agcataagcg agtggacaga aagcatggcc caagcgagta ttgcgaaagc aggatgttct
    18301 gcatcgtgat ggtggtatag ttcttttccc aaatgttcaa accacaagaa aataaggaat
    18361 actactccgg taataataaa cgcaaacgga tagttataac ccatttcttt aaataaggtg
    18421 tttgattcag gtaacatgtg aagaagtgct gcacccagaa aaacacctgt agccaaagtt
    18481 tcacctatgg gaaaatcaat gtgtttatcg tctttaagtc gctttttaaa tggataccag
    18541 cctgcaatga gaatgactat aaatatcgaa acagcaaaaa tgatttttaa accggttgcg
    18601 gacaacatga agtcttccta ctttaaaatg cgtatatatt atcctgaaat gactaatcga
    18661 atctacaggg cacagcagta ttacattgtt aaaatttagc aattcttgca tgccttctga
    18721 atcttagttt gatggatgat gcatctcttc atgcaagcca aagagacgca cttcctttat
    18781 atcgatttgt gacttcaggg ttgaggttac tcgattatgc aattttttcc attgattaaa
    18841 atcatttgag caaaaaatat ctaaactcat ttttccattt aaataatgta tattccaaaa
    18901 taaaatctgg ggaaaatcat ggcgaatgtc attaaaaaat ttttcctgga gtattgccct
    18961 gtttggtaaa tgcaaggatg gacatgaaat ttcatcgtct tcagggtcaa catggacaat
    19021 cacatctttg acgcaatcaa tttcttttac cagagtatga tgcacatgtt gggcgatata
    19081 gtgcccctcg gagacggata ttttgggtga aaccagaata tggacatcaa taagcacatc
    19141 actacccata aaccggcttc tcaattggtg aatacgctgt actccatcaa tgctttgtat
    19201 aactcgttca atttgagaaa ataattctgc atcaacagct gtgtctacca actctttgac
    19261 actgttccag ccatagctcc accccatttt tataatcatc aggcttacga taacggcggc
    19321 aatagcatcc aggtaaacaa gtcctgccaa gctgccaatt aatccaatca gcacaactat
    19381 ggatgaagca gcatctgatc gatgatgcca ggcattggcg gtgattagtt gggagctaat
    19441 tttttttcct acatggcggg tgtagtgaaa taatgcttcg tttgcaatga ttgagaagca
    19501 cactatagga atcgataacc agtttggcat ggtatggtct gatttaagca attcatcaag
    19561 tgcatcccag gcaatcccac agcctgccag gattagtaaa agggaaagca ataaagttgc
    19621 tgccgtttca attctttgat ggccatatgg atgagagtca tcagcgctaa cgcttccata
    19681 ttttgaagca aatacaacca tcacatcagt aattaaatca gaaatggagt ggaccccatc
    19741 cgcgatgaga gcatgagaat gatataaaaa accaccaatc attttgacaa caccgagtaa
    19801 ggcattaata aaagcaccga taagagtcac ttttttggct tgccaatacc tatcatgggg
    19861 gtacattgtt gttccttttc aatcacaaaa ataatttgga tttttgtaaa taaaaattag
    19921 aagcgaaact ataccatgtt atagcatgag ttcctattta tctcgtgctg gcagttatag
    19981 tcgactaaat gtattaataa tttctttttg gtttttactc gattacgcta ggagaagtta
    20041 aaaaaataat tgcagatgtt acccaaaagc aaattggaaa gttctgaatc agttagaaaa
    20101 ttgactgatt ttacaaggtg ttttgcattc tgtgcacagg attatccaca gattttgtgg
    20161 ataaatatat ttgagtgaat taaattaaaa aagccaagta acccttacat ggaaagggct
    20221 atagaaattt caatagtaaa attagcaaga gttttgtgca attgattttt aaagttatcc
    20281 acaaggataa aatggaatga attttgtttc aggcagcctt atgttagcaa agaaaaaaat
    20341 aaaaaacagt cttgacttca ataattttaa ttattttttt aactttgcaa aaattgttca
    20401 aaattgccca gccatcgttg tgtaatttgt ctggcgattg ctgttttttc cagtaccagt
    20461 tgttttgcaa ttggacgtaa aatagcgaag agggttttat cgcgttgtaa gttagctatt
    20521 ttaaattgtc taaagccagt ctgtctggtt cctaatattt ctcctgagcc tcttaactcc
    20581 aaatcctttt cggagattag aaaaccgtct gttgttgaac gcatgatctt gagacgttca
    20641 gcaccttgct gtgatagagg agattgataa agcaatagac aatgagattg attattacct
    20701 ctgccgacgc gcccgcgtaa ttgatgtaac tgcgacaaac caagtcgctc cgcattttca
    20761 atgatcatta aactggcatt aggaacatct acaccaactt caataactgt agttgccacc
    20821 aataaatcaa tctcgccttg tttaaaggcc gccatagtag cttctttttc aagcgctttc
    20881 attctcccat gaataagacc aacacgcgcg aaagagagct gttcttgtaa tttttttgag
    20941 gtatctgttg ctgctatgca ttgtaatttt tcagattctt ctataagggt acagacccag
    21001 taagcttgtt tgccgttggc gattgctgct tgcaaacgtt cgataatgag ttcacgctta
    21061 tcctggttca ataccgctgt tgtaattggc atacgtccag gtggtagttc atcgatgacc
    21121 gatatatcca aatgagcaaa atgagacatt gacagggttc ttggaatagg ggtggctgtc
    21181 attaataatt gatgagggat taattggttt aattgccctt tttgttgcag aaggagccgt
    21241 tgttctactc caaatcggtg ttgttcgtca ataatgacca aacccaaaca agcgaattcg
    21301 acttgctcct gaaataaagc atgagttcca acaatgagct gacagctgtt gtcctgcagg
    21361 gctgcaagcg cattttttcg ttctgtggct ttcattttac cgctcagtct taatacatta
    21421 ataccgatag gttctaacca tttactgagg ctatttgtat gttgttcact taataaatcg
    21481 gttggtgcca taaaagcaac ctgaaatcct tgtgaaatgg cttgaagggc ggctagtgcc
    21541 gcgataatgg ttttacccgc cccaacatct ccttgcagca aacgaagcat gggtttgctt
    21601 tgagttaaat caacgctgat ttctttaaaa acacgttgtt gtgcgtttgt aagactgaat
    21661 ggcaatgatt cgataaaacg tttatgaatt gcattatcga aaagtatagc gggagcttgg
    21721 agtttgctgc gtgactgtct tgcaaattgc atactgagct gctgtgccag taattcatca
    21781 aatattaacc gcttgagagc aggatgttcg ccggcttcga ggttggagag ggagatatct
    21841 ggtggagggt tatgtagtaa tttaatggct tcgcccaaat cataaaaatt attttcctgt
    21901 aattgttttt cactcatcca ttccaattga tgaagttcgt gttcagattg ttccagggct
    21961 attttaacta actgccttaa tcgggtttga gtcaagcctt gagtcgatgg ataaattggc
    22021 gtaagcgttt cttccacgtg gaaatctgat tcttggtcaa ttaattgata ttctggatga
    22081 atcatctcca attggttgtt aaatccgcgg acttcgccaa aagctcgaat catggtgcta
    22141 ttattcaaag cctgaatttg ctgcttattg aaatgaaaaa aacgaagctt aactactcct
    22201 gttttatctt ccacatagca attcaacatc atccttttgc cgtattttat ttcagttttg
    22261 catacatgcc ccgcgataac acaccattca ttggagcgta aatcctgaat tggtgttatc
    22321 ctggttctgt cttgatattt ataaggcaag tggaaaagca agtcttgtat cgtgaaaatt
    22381 ccacatttag caagttttgc tgcaatggtt gggccgacgc cagttaaata ttcacaagag
    22441 ttactcagca cggtattatt taagattaaa aatttaataa taacaggaaa atatattcgt
    22501 tgcgacttga gtcatcattc tggcaatttt gcccaaatca ttactaagtt acttgttgct
    22561 tgcgagtatc tattcatcaa agatggagtg taattggatg aatcgtatta taacgttcac
    22621 agcgggtttg atactggttt ggataacagg ttatttatta atcgcaggaa gaggactatt
    22681 aattccaatt gttatatcca tttttgtctg gcatctgttg aataccatta atagcggtgt
    22741 tcagaaaatt cctttcatcg gcacacatct cccatcctgg ataaggatga ttttatcttt
    22801 gtttgttgtt gtttgcctcg taatggttct tattaacatc attaccaata atgtcagtga
    22861 tgtgatagcc gcttctccca ggtatcagga aaatttactg cacatattta acagcctgga
    22921 tcaacgattt catgttaagg gtatttttga tttcgacagt ttaattcaag ggttaagcat
    22981 tcagagtatg cttgttaata tttatggggt ttttacgaca ataaccagtt ccgctgtact
    23041 gatttcctta tatgttgtgt ttctgtttgt cgagcagcat tattttactc aaaagatgga
    23101 tgcctttttc cctcaggcag agcacaggaa gctggttaaa aacattatct cccatattac
    23161 aaaagatact caaacttatc tgggtattaa aactttgttg agcctgctca ctgccacgtt
    23221 gagttggttt attatgaagt gggttggatt ggattttgcc gagttttggg cattattaat
    23281 tttcttttta aattatatcc ccaatatagg agctattgta gcaacagctt ttccagctgc
    23341 ccttgctttg atccaatttg aaagctggtg gccgtttttg gaagtgactt ccgggatagt
    23401 ggctatacag tttttcgtgg gcaattttct agagcctcgt ctattaggta aatcactaaa
    23461 cttaagccct ttagtgatat tgtttgcctt agcgctatgg ggtgccattt ggggggtact
    23521 aggcatgttt ttgtctgtgc ctatcactgt aatgatgatg attatttttg cgcactttga
    23581 tgtgacaaga cccatagcaa ttctgctgtc tcaagatgga tatattcaca aatcctatga
    23641 atctcttgaa aattaatgag ctattgctgt ttctattgtt gtagtgatgg tgcttctggg
    23701 ttgagagcct ttccatatgg tatgaaaaag agcataatat cggtattttt gacggataaa
    23761 tgagaagtat gaaagctata atcgaatatc ttttgaaaca ggcattaatt aatctgcaac
    23821 aatcaggcga aatgccaatt gatttggagg ttgaaataaa agttgagaat gccaaagatc
    23881 catctcatgg tgattatgcg actaatttgg cactggtatt agccaaacca tgccgacaag
    23941 cacctaaagt attagccgag cgattggttg cagtaatccc cgcggatccg tctgtagaga
    24001 aaattgagat agccggggca ggatttatta attttttcat gcgcagcacg gcccgctcgc
    24061 tcatcatttc tgaaattttg aataaaggca aggagtttgg ccgaggtaat ttaggacaga
    24121 gtcagaaagt attgattgaa tttgtttctg ctaatccaac agggccttta catgtaggac
    24181 atggtagagg agctgctttc ggagcaactt tgggtaatgt tttaaaagct gctggttacg
    24241 atgtgacttt ggaatattat gtcaatgatg cgggcaggca aatgaatatt ctcgctgtga
    24301 gtgtgtggct gcgctacctg gaattggcag gtgagccaat tgtttttccg acgaatggct
    24361 ataaaggtca atacgtttat gagattgcac aagagatgtg gtcagagcag ggtaatcaat
    24421 tcgtgcatcc atggatttcg gtagtagaaa atttacctgc tgatgaaccc gaaggtggag
    24481 ataaagagac ttatatcgat gcgatcatcg cgcgagcgca atccttactg ggcaaggatg
    24541 ggtttgctaa cttccatcaa catgctttaa aaacggtatt ggatgatata aaagatgatt
    24601 tgcaggcgtt tggtgttcgt tttgacagtt ggttttctga gcaatcgcta tttgaagatg
    24661 gttctattga gaaaggtatt caggccttga aagatagagg ccacacttat gaaagagaag
    24721 gcgctttatg gtttagggca actgattttg gtgatgaaaa agatagagta ttagtccggg
    24781 ctaatgggca aacgacatat ttcgcttctg atgttgctta tcactggaat aaatatgata
    24841 gaggttttga tcgagtcatt gatatttttg gtgctgatca tcatggttat gtcacgagaa
    24901 ttaaaacagc tgttaaggca ttaggacatg atgaaagtgc attggatgtc attttggtgc
    24961 agtttgccat tctgtatcga ggtggtgaca gggtacaaat gtctactcgc agtggttcat
    25021 ttgttacttt gagagagcta agagaagaag taggaaatga tgcggctcgt tatttttatg
    25081 tggcaagaaa acctgaacaa cacatggatt ttgacctgga cctggcaaag tccgaatcca
    25141 gtgacaatcc tgtgtattat attcaatatg cacatgcccg tatctgcagt gtattgagac
    25201 agttaaaaga aaggggtttg aagtgggata aggatatggg acttaaaaac cttgatttat
    25261 tggagcaaca acatgagact acattaattt ctctgattgc ccgatatccc gaagtcattc
    25321 aatctgccgc tgcttcttgc gagccgcatc agcttgctta ctatttaaga gagctagcca
    25381 atgggttaca cagttattat aatgcgatcc agctattatg cgaacaagaa caattgcgat
    25441 gtgcccgttt gtgtttgctg gagtcggttc gacaggtttt aaataatggt ttggctatat
    25501 taggcgtatc agctcccgag agtatgtgat ggcaaaagat tatggaaata ggcgtgctgc
    25561 acgtcgcaac agaggaccgc accagttttt ggttattatt gtgacttttt tgtttggata
    25621 tataaccgct tcgtttctcg atgtacaaac gataagtcaa tgggtgaata cacaggtttt
    25681 agctcaacat gatgatgaaa aagcgccaac taaagtggcg caagaacata aacaagtgcc
    25741 tcctaagcct aaatttgagt tttatacatt attagcgaat gaaagggggc cggcatctca
    25801 ggcatctagt cagcaaaata cagacaatca tgcggcagat aagacatcga ctccaataca
    25861 aaagactgca aatactgtca ctgcagctgt aaataactca gtgaaatcaa cgataactaa
    25921 tccaacaccg caaaccccag tgaaggtagc ggaggcaaaa ccaacaacca tgcaacaacc
    25981 taataaagga gcatatttgg ttcaagtcgc atcttttaaa gcgcgtcagg atgcagaaca
    26041 aatgaaaggg atgctaatcc ttaagggttt tgatgtcagc gtggttccag tcacccaagc
    26101 acagggaaat tggttcaggg tagtggtggg cccctatcca aataaggcct tggctcaaaa
    26161 agctcaaatg aaccttgcca aaacagagca cctgaacggc atggtgcgca gcgttggggg
    26221 ataaggagta atcatttaat caaagaagat tgcttctttc agattatctg tttacaacta
    26281 cttggattcc cgcagcgttt tttacagcat ggggttttgt aacagcgacg gaaaccttac
    26341 taacaggata cttttgcttg attaattgtg caatttgttc cgctaccgtt tcaattaatt
    26401 gaaacgaagt tgactctaca aattgagtta ctgtttggca caaatcagcg taatccagtg
    26461 tattagacaa attatctttg caatctttaa agtcaaaggg gatggatata tcaattaata
    26521 atcgttgatt aatacgctgc tcccacccat aaacaccaat ttttgtttca acactgagag
    26581 ctgtaatatt taaagtatcc atttgatatc cattaataat ttatcaggat ttacgaaaaa
    26641 tcctcaatct ttttgtttaa aattttttaa ctcagtcatt taatttatct aaactttctt
    26701 atgtaaatat tgtttgcctt gagcttggag tttttcgtgt gtcctgctta tattgttaag
    26761 tatcgattgc tatttatacc actgtattga ttaccttaac atgatactat aaacactttt
    26821 tattgtgata gtgattaaat gatgtttgat ttgaaccaat ccctttttct tcgagctttg
    26881 agacgacaac ctgtggagag aactccaatc tggattatgc gccaggctgg gcgttatttg
    26941 cctgaatata gaaaagtaag agagcatgct ggtgattttt taaatctttg caagaatccg
    27001 gaactcgcct gtgaagtaac cttgcaaccc ttgcgtcgct atgctttgga tgcagctatt
    27061 ttattttccg atattttaac aatacctgat gctatggggc tagggctcta ttttgccgaa
    27121 ggagaaggac cttgtttcac aaatccttta caagatacca aggcaattca tgccctgaaa
    27181 attccttcca ttcccgaatc tttatcctat gtctttgatg ctgcgcgctt aatccgccag
    27241 gaaatgccta aagaattacc tttgatcggg ttttcaggta gcccatggac tttagcttgt
    27301 tatatggttg agggtgggag cagtagagat tttaagcgaa ttttaaattt aatatacact
    27361 gaaaaagaag cagcgcacct gttattgaat aaattggctg tctctgtgac tgcctattta
    27421 actgagcaaa taaaggcggg agttaatgcg gttatgatct ttgatacgtg ggggggagtt
    27481 ttaactccac aaaattataa ggatttttct ttggcatata tgcatcaaat tgttcagcaa
    27541 ttgaaaaacg aataccctga tatccctgta attttattta ccaagaatgg tgggcaatgg
    27601 ttagaatgga tggcagaaac tggttgtgat gcattaggag ttgactggac gtgtgatttg
    27661 gcttccgcac gcaagagagt aggcggaaag gttgctttac aaggaaatct ggatcctgct
    27721 gttttactaa ctgcaaaaaa ttgcatacga agagaggttg ggagtgtact ggcatcttat
    27781 ggttatggaa cagggcatat ctttaatttg ggccatggca ttactccgga tgtaccccct
    27841 gagaatgttg caataatgat tgaggcggtg catgaaataa gtcctcaata tcatttgtag
    27901 cctgtgcgag gcaaattatt gtttcaaaat agtgaaatgc ctcaagaata agacaatcgt
    27961 aactgtacac ggggtatgca agtttttctg tttataagcc tatttatttg agttgtcttt
    28021 cccgcgcagg cgggaatcta actaaaaatc aatctatgtg ctagctttat ggatagattt
    28081 ccgcctgcgc gggaaagaca gggcgcttaa tattccaatt acataccctg tgaacagtta
    28141 caaacaatcg gcttttggcg tagatactgg ccttcattca agacgttatg agtttcatta
    28201 aggttcactt ctgaattgat ggcttaattg gatggccatt tccagagatt gttcatagtt
    28261 taatcttgga tcaaccaggc tgtgataggc agttttgagg tcatggggag ctaatccacg
    28321 agcgcctccg atacactctg ttacattgtc accagttagc tcaaaatgga cacctccgag
    28381 gtagctaccc atgctgcgat gaatttccaa agcttgtttt aattccgata aaatgttatc
    28441 gaagtgacgt gttttagtac cgtcttctgt tgtttcggta ttgccatgca tagggtcaca
    28501 tgaccatgtg actggaattt tgcttttcct tactgtgtca atcagtggag gtaacaaccg
    28561 gtcgataagt tttgctccca ggcgagtata aagtaaaact cgtccttctt ctttttgagg
    28621 attggctacg cttaacacct ccgataacca ttcaggtgta gctgctgggc ctatcttaat
    28681 gcctattgga ttttgtaccc ctcgtagaaa ctcaagatgt gcactgtccg tttgagccgt
    28741 acgcattcca atccagggta aatgagttga aagattgtac cattttccat ctttcaactg
    28801 tcttgttaat gcttgctcat aatgtaaatg caacgcttca tgagaggtgt aaaaatcaac
    28861 tttacttatg ctactcgaat gtattccatc tatggcattc aaaaaatcca gggcgtcttc
    28921 aatagagtct acaatatgtt ggtattcgtt cttctgaggg gagtgttcaa caaaacctaa
    28981 atcccagcgt tgagggtgat gcaggtcagc aaaaccgcca tccagcaacg ctcggataaa
    29041 atttaaagtc atggcagaac aactgtatgc ctgaagtaat aatttaggat taggctccct
    29101 ggcagtggca gtaaattcag gtgaattaac gatatctcca cgataactgg gtaaagtaac
    29161 tccatttatg gtttcgtaat ctgaggagcg tggttttgca tattgtccag ctattcttcc
    29221 gatacgaata attggtttac gcaagccata taataagatt aggctcattt gaagaatgat
    29281 ttttaattta ttgcttatga cttctgaacg acagtcatta aaagattcag cgcaatcacc
    29341 accctgcaga ataaaagcat tacctcggcc agcctgggca atttcatttt ttaaatgttt
    29401 gatttcaccg ctagtaacca atgggggtag caaacttaat tgttctacca ctttatttaa
    29461 ttgctcttca tcagcgtatg aagctgcctg caaatacgaa tatttctgcc atgtagttgg
    29521 cgaccattct tgcataattt acccaaatta tttttgcttt agttgtcgtt tagacttaat
    29581 ttaactcaaa ggattattaa atactttgat ttttttacct aaatgagaat taagacagat
    29641 ttttttgcta cctaagtatg gaataaatgg acaattactg caagccatga tttgaaaagt
    29701 ttcaatcagt taatgacctg agttttaaac caagatttaa aatcttaatt ggggcttgaa
    29761 aattgcaaat acaatcccca tttgcagaaa tcgagttagt caggagcgat tttgatgagt
    29821 aaacaaaata aaaaagattg gaaaaaattt aaagacgaac acaaagaaga acacaaggta
    29881 gagaatgaga ttctggaaga agaaattgat gaaaaatcac aacatcaaga acctgcttta
    29941 ggacatccca gctatactgc cttggaagag caactgacac tggcggaaca aaaagcacat
    30001 gaaaattggg aaaaatctgt acgcgccctt gctgaattgg ataacgttcg tcgtcgtatg
    30061 gaacgagaag ttgccaatgc ccataaatat ggcgtagaaa aactgatttc agctttattg
    30121 ccagtagtgg acagtcttga gcaagcatta caattagcgg acaagaacag tgatccatct
    30181 atgcatgaag ggcttgagtt aaccatgaaa ctctttcttg acgcattaca gaagtttgat
    30241 gtggaacaaa ttgatcctct tggacagaca tttgaccctc aacaacatga ggcaatgtca
    30301 atgcaacctg ccccaggagc gccaccgaat tctgtaatta ctgtattcca aaaaggttac
    30361 aaattgagcg atcgagtaat tcgaccagct cgggtgatag tttccaccaa ataatctggt
    30421 gtttcagttg aggtattttt aaacaaattt ttagacgcag tgtattcagc tttttttagg
    30481 gtgatagctt gaaatcgtca tgatgcaccc catatgtgga atattgcatg agaaaatatt
    30541 aatttggagc aaaatagaat ggctaaaata ataggtatag acctgggtac tacgaactct
    30601 tgtgttgctg tcatggaagg tgacaaaccg aaggtaattg aaaatagtga aggtcacaga
    30661 acaactcctt ctatcgtagc atttaccgat gacaatgaaa tattggtagg ccaatcagcc
    30721 aagcgtcaat ccgtaactaa cccagagaaa acactgttcg cgattaaacg attaattggg
    30781 cgtcgatttg atgatcctat tgtgcaaaaa gatattaaga tggttcccta caaaattatg
    30841 aaagcggaca atggtgatgc ttgggttaga gtcaaggatc aagataaggc accaccacaa
    30901 atttctgctg aagtgctaag aaaaatgaaa aaaacagcag aagattattt gggtgaagaa
    30961 gtaaaagaag cagtgattac tgtacctgcc tatttcaacg attcgcaacg ccaggcaacc
    31021 aaagatgctg gtcgtattgc tggcctggag gtaaaacgaa tcattaatga gcctaccgct
    31081 gccgcattgg cttatggtat ggataaaaaa cgcggtgact ctgttattgc ggtatatgat
    31141 ttagggggag gaacttttga tatttcaatt attgaaattg ccgaagtgga tggtgaacat
    31201 caatttgaag tacttgcaac caatggtgat accttcctcg gtggagaaga ttttgatttg
    31261 gctttaattg agtatttagc ttctgagttc aagaaagaca ctggtattga tttgcataat
    31321 gatcctctgg ctctgcaacg cttgaaagag gctgccgaga aagcaaaaat tgaattgtca
    31381 tctgcacaac aaactgatgt caatttgcct tacattacag cagatgcttc tggaccaaaa
    31441 catttaaata tcaaattgac tcgcgcaaaa ctggaatctt tagtggagaa actggttgag
    31501 agaactattg agccatgtaa aacagcttta aaggatgcag ggcttactgt ctcacaaatc
    31561 aacgaagtta ttctggtcgg tgggcaaact cgtatgcctc tggtacaaaa aactgtggaa
    31621 gaattctttg gcaaagaacc tcgtaaggat gtaaaccctg atgaagctgt ggcagtaggt
    31681 gctgcaattc aggcagcagt attgtctggt gaagtgaaag acattctttt actcgatgtc
    31741 actcctttgt ctctcggtat agaaactatg ggtggtgtaa tgaccaaact cattgaaaaa
    31801 aacacaacta ttcctacaaa ggctacacaa gtgttttcaa cagccgatga taatcagaca
    31861 gcagtgactg tgcatgtatt gcagggtgaa agagaacagg cttcagcaaa caagtcatta
    31921 ggacggtttg atttgcgtga catccctcct gctcctcgtg gtgttcctca aattgaagta
    31981 acttttgata ttgatgccaa tggtatcttg aatgtttctg ccaaagacaa ggcaactggc
    32041 aaggcacaat ccattgtcat caaagcttcc agtggtttaa gtgaagaaga ggttgctgct
    32101 atggtgaaag atgcccaatc gcatgctgaa gaagataaga aattcaagga aatggcagaa
    32161 ctgcgcaatc aggcagatag tttgatccat agctgtgaaa aatccatgaa agatttggct
    32221 gatgagttgt ctgaggatga gaaaaaaggc atcgaaacag caatttctga gctgaaagag
    32281 gcggtgcaag gaactgacaa aactcgtatt gaagacaagc tgaaagtatt gacagacgct
    32341 tccgcaaaaa tggctgaacg tatttatgct aaaaaatcgt ctgaaggtca ggcagctcag
    32401 gggcaaacac aatctcagga atctacaaaa cctgtggaag agggagttgt tgatgctgag
    32461 tttgaagaag ttaaagaaga ggataaaaag taatttaata ttactctcgc cctctgattt
    32521 aagagggcgg aagaaggatt catcgggtta taataaataa attttgggtc ggggcgacat
    32581 tatcgtcccg tttgtctatt gaatagcact taagctaatt tagagtcttc atacgactga
    32641 gagaattata attaagtgat cgttataaat tgtgagcagt tatggaacag cgggattatt
    32701 atgaactttt agaagtaagt cgaaatgcga gtgatgcaga gattaaaaaa gcatatcgca
    32761 ggctggcaat gaaataccat cccgatcgga atccaggtga tacctctgcc gaagaaaaat
    32821 ttaaagaaat tcaaaaagcc tataatatct tgtccgataa gcaaaaacgc gccgcttatg
    32881 atcaattcgg ccatgccgga gtggatcctt caatgggtgg cggcccaggt ggttttggtg
    32941 gatttggagg atttggtgac gtttttgaag atatttttga gaatattttt tcaggggggc
    33001 gtgggcatgg gcgtcaatca cgtggacaaa gaggcgctga tttgcaattt aatgtgcaat
    33061 tgacccttga agaagcagct ataggaaaag aagttgaaat taccgttcca agacatggta
    33121 cttgtactgt ttgtgaggga tcgggggcta aaaaaggcac aagcccaaaa acttgtgaaa
    33181 catgccaggg catggggcaa gtcagaattc aacaaggttt tttttccata caacaaacct
    33241 gccccacttg tcatggtgag ggaaaaataa tttctgatcc ttgtgccagt tgtcatggtc
    33301 aaggaagagt tagagaaagt aaaaaaataa atgtcaaaat ccctgctggc gtcgataatg
    33361 gtgatcgagt ccgtttaagc ggagaaggtg aagcaggggt gcatggtggt ggttcaggag
    33421 atttatatgt tcaaattagc ttgaaaaaac atgctatttt tgagcgccat gaaaatgatt
    33481 tacattgtga agtacctatt agttttgcaa ctgcggcgtt aggcggctca atcgaggtac
    33541 ccactttgga aggccgagtt actttaaaaa ttccagcgga aactcaaaca ggcaaagtgt
    33601 tccgcttgag aagtaaaggg atgaaatctg tcagaggata tggacaaggc gatttgctat
    33661 gcaaggtagt tgtagaaaca ccggtcaatt tatctcgaga gcaaaaagaa ctgttaaata
    33721 agctacagga ttcattagag aatgccaagg gaacgcactc acctaaaaca agctcctggt
    33781 ttgctggtgt gaaaaaattc ttcgaagaca tgaaattttg acttaattaa gttacaattg
    33841 gaagatttgt cgttttcagc atggattgcc tattttacgc tggataagca tactttgaac
    33901 ataattgagt atattcatga ttaataaatc agcaatttta gctttagctg atggcactgt
    33961 ttatgagggc ctgtcagtag gagccactgg tgattctgtt ggtgagttgg tttttaacac
    34021 gtcaatgact ggctatcagg aaatgcttac tgatccttcg tatgccagac aaataattac
    34081 cttaactact gctcatgtgg gaaatacggg ttgtaacatg gaggacatgg agtctacaaa
    34141 agtctgggca gccggcctcg tcatgagaaa ctgcactgca atccctagca attacagagc
    34201 tgaacaatcc ttcccagact ggttaagtaa aaatggagtt gttgcaattt ctggcattga
    34261 tacgcgcgcg cttacactga aattgcgtga gaagggttct atcggagctt gtattagcac
    34321 caatgtggag aaacctgaaa ttgctgtggc aaaggctaaa tcctttgcag ggcttcaggg
    34381 tgcggatttg gctttagagg ttacaagaca aacgatagag cgttggcatg aaggtcgggg
    34441 aatgtggagt ctagacaaac agcctcaaca gcttcatgtt gttgcctatg attttggtgt
    34501 gaaacataat attttacgta tcttacatga taagggatgc catattacta tagtccctgc
    34561 taaaacttca gcagaagagg ttttagccat gaatcctaat ggtgtctttt tatccaatgg
    34621 accgggtgat ccccaagcat gcgattatgc catcaaagca acacaggtat ttttagacaa
    34681 taatattcct ttatttggta tatgccttgg gtttcagatt ttggctttgg catgtggtgg
    34741 tattactaaa aaaatgaaat ttggacatca tggttccaat catcctgtag ttgaaacaac
    34801 tggcaaaaaa cgtgttttta taacaagtca aaatcatggt tttgctgtag atgaggattc
    34861 attgcccaat tgcctggaag ttacgcatcg ttcattattt gataacagtt tacagggaat
    34921 aaagcacaaa acaaaaccgg catttggttt tcaaggccat ccagaagcaa gccctgggcc
    34981 acatgatata gatattttat ttaatgaatt tgtaaatctg atgaaataaa tttttagctt
    35041 cagaattatt attttggcaa gagtggcttg ttttaagcct gcttacaatg attgtaatac
    35101 ccctaaatgc tatccatggc tattaaagat tagtaaaatg acatggattt accaaaacaa
    35161 tgactttaac ttgttttgtg ttttaagcag agataaagat tagggatact aaaaatagaa
    35221 aatacagagg atatgtaatg aatgaagtct atgtttttac ctcagagtct gtttccgagg
    35281 gccatcctga taagatagct gatcaaattt cagatgccat tttagatgct attttagccc
    35341 aagatccaaa agcacgagtg gcttgtgagg tgttggttaa aaccggtatg gttttggttg
    35401 gtggagaaat tacgacgaaa gcttgggttg atgttgaaga aataactcgt catgttatca
    35461 aggacattgg ctataatagt tcccagatgg ggtttgattg ggaatcttgt gctgtgcttt
    35521 cagcgattgg caagcaatca ccagacatag ctcaaggtgt cgataatcag caaactaaaa
    35581 ttctgggtgc tggcgatcag ggacttatgt ttggttatgc cagccgtgaa acagatgttt
    35641 ttatgcctgc ccctattgcc tatgcgcatc gtttgatgga gaaattagct aaagctcgta
    35701 aatcaggcca attaccctgg ttaagacccg atgcaaaatg tcaacttact ttgaaatatg
    35761 aacaagggat gccggttgaa gtggatactg ttgttttttc tacgcagcat tcacctgata
    35821 ttgagcataa agatttggtt gaggcgatcc gtgaagaaat tattaaatca gttcttcctg
    35881 ctgaatggct taatgataag acccggtatt tcattaatcc aacaggacgc ttcgtaattg
    35941 gtggtccttt gggcgattgt ggcttaaccg gccgtaaaat cattgttgat acttatggtg
    36001 gtatggcaag acatggagga ggctgttttt caggtaaaga tccttcgaag gtagatcgct
    36061 ccgcagcata cgccgcaaga catgttgcca agaatattgt ggcggctggt ttggcagata
    36121 aatgcgagtt acagatttcc tatgctattg gtgttgctga acctacatct atttttgttg
    36181 atacttttgg tactggacga ctaaaaaaca gcgaaataat tgatttaatt catacccatt
    36241 ttgatttaac accgcaaggt attattgatc aacacgattt gcttcgtcct atttataggc
    36301 agacggcaac ttatgggcac tatggcaggg agaattttcc atgggagcga ttggacaaag
    36361 tagctgaatt aagcaaggca ttataatttt ttaaggatac tctaatggaa ctagtagaag
    36421 aaggagtaca gaaaatcaat acattacagg attataaagt agcagatatt tcattagcgg
    36481 actgggggcg aaaagaaata gcaatagctg aaaccgaaat gccgggttta atggctttac
    36541 gcgaggaatt ttctgcgaga aaaccacttc aaggcgctcg gattgcaggt tgtctgcata
    36601 tgacgattca aaccgcagta ttgattgaga ccctggttgc attgggtgct gaagtacgct
    36661 ggtcttcctg caatattttt tctactcagg atcatgcagc ttccgctatt gctgcaaaag
    36721 gaatccctgt ctttgcctgg aagggcgaaa cagaagaaga atattggtgg tgtgttgagc
    36781 aaactttaag tggccccaat ggttggacac ctaatttatt attagatgat ggtggagatc
    36841 tgacacaagt tgttcaccag aaacatccta aattactgac tgcaattaag ggagtatctg
    36901 aggaaaccac tacaggtgta gccaggttgt acgaaatggc gaaacatgga caattaaaaa
    36961 ttccagccat taacgttaat aatgcggtga ccaaatccaa atttgataat ttgtatggat
    37021 gtcgggaatc cctgcttgat gggttaaaac gtgcaacaga tgttatgata gctggtaagg
    37081 ttgccttgat tctaggctat ggtgacgtag ggaaaggttg cgctcaggcc ttgcgtggac
    37141 agggagccac agtacttgtc gcagaaattg atcctatttg cgcacttcaa gcggctatgg
    37201 aaggctacag agtcgtcact ctggatgatg ttgctgaaca agtggatatc gtagttacag
    37261 ctactggcaa ttaccacgtt gtaactcacg atcacatgaa acgtatgcgt aatcaggcga
    37321 tattgtgcaa cataggccat tttgattcgg aaattgatat tcaaagctta aaacagtatc
    37381 aatgggaaaa tatcaagcct caggttgatc atgtgatttt tcctgatgga aagcggatta
    37441 ttatcctggc tgaagggcgt ttagtgaatt tgggctgcgc tacaggccat ccaagctttg
    37501 ttatgtctgc ttcctttacc aatcaagtat tggcgcagat cgagctgttc caaaactcta
    37561 gccaatacca gaatcaagta tatgtacttc caaaagtttt ggatgaaaaa gtagcccgtt
    37621 tgcatttggg gcgtattggt gcaaaattaa ctcaattaag taatgagcag gctgattata
    37681 tcggcgtcga taaaaatgga ccttacaagc cggatcatta tcgttattaa actaatttta
    37741 ttcaagattt agtcatgaca actaaatctt gaattttttt acattattgg atcttatact
    37801 ctaatataga ttagaattaa ttcacttctc acttaaaaaa gaaaagcctg ttgatggatc
    37861 tggttatagc ggcaaattat ctttctactc tggaaaaatg tcttgaacaa tttgggatta
    37921 accatttttt agaaaaaatt ggctttgatc tgataagagt acatgatcct gatgcctact
    37981 taactcagga tgaatttaac caggttattt tggaagctta taaattaagc aattgcccgg
    38041 aattgggttt ggtttttggt caaaacttat ctatagtcaa tcacggtttt cttgggtatg
    38101 cagcaatgag cagtcctaca tttggaactg ctatacagac tgtgctcagt taccttaata
    38161 cacgaaccaa tttgttgtct ctggagttac gccagtttaa taatgaaaac agagcttata
    38221 tcaagttatt accgcacaca aaagaaatgg ttttatcacg ttttataact gaattggcag
    38281 ttactcatct tattaaaatg agaaattttt tgattagttg taatgacccg ttgctcaaga
    38341 ttgagttaaa ttacgatgaa cctccctatg caaaatatta tcaaacaatt tttcagacta
    38401 aaattgtttt taatacagag gctactcgcg tttggtttaa agcggaggaa ctgaattacc
    38461 ccatccattt tgctgacgat gtcagctatc agctggcaaa aaggcaattg caagaacttg
    38521 aaaaaaaaat gtcattaaaa gaggacatgg caagcaagat taaatctatt ttaatcaatc
    38581 agaatttaaa tcatgccagc atggatgaga tagccagtcg attatgtatg acttctcgta
    38641 ctctaagacg tcatttgcag agattggatg tgacttatca agagttgctt gatgaggtaa
    38701 gagaacaaaa agcaaagaca tttttgctca ataataatat ttccatgacc gagatcagtt
    38761 ttttattggg ttttcaagat acatccaatt tttcaaaagc attcaaacga tggacaggag
    38821 tcactcccac agaatatagg gaaaaacatg aagtccaatg attgttccat cttaatttaa
    38881 tctcatggct tattgattct tgagttgata tttattctcc tgcatactga ggtggtttta
    38941 tttttaaaaa ttggtcaagg ctacggattg actttaaaaa ggagattgaa gtatgggttt
    39001 gaaatcagga atattgggat taagtctttt atcgcttgct tgtggcagtt ttgctatgac
    39061 tgaaactgaa acaatcagag ttatcattaa atataaacag caaccagcca ccatggcttc
    39121 actaaaatca caagtcatta aatctgttca aatacccgtt aaagaaatga agcctatggc
    39181 caatggtgct tattcgctta tttttgatac atcgaattcc catgctattg tcaaggctaa
    39241 ggaagaagat gttacagcca aagttttaga acgtttgcgc aaaaatccca atgtacttta
    39301 tgcagttaaa gacagaatag gacattttaa gcctctccct gatccagtaa taaaccagga
    39361 gttaagtcaa ttgttatccc atgagagtca atgggatgaa ttcgaaaggc cagcgggaat
    39421 tcgtttggaa tccaagcctg gtttaagaga tggcgcatgg gcatatacta caggaaagtc
    39481 caggaagccg atagttgttg cggttttaga tactgggatt gcattgaatg acagtttggt
    39541 gaataacctt gtaaaggata atgagggtaa tgtatttgga tggaattttg cagggaataa
    39601 caatgattta tctgatgaaa ccagatctta ccatggcacc catgttgccg gtaccattgc
    39661 gggttatggc gatgttatga acggagttgg tgaggattta aaaattctga ctgttaaaat
    39721 tcctgccgag aatggtatgt tttatgaaag tgctgtggtg aatagtatct attgggctgt
    39781 tggaggtgat gttccgggag ttcctaaaaa tatacatccg gccaaggtat taaatatgag
    39841 ttttggtgtc gatttagagc ctggaaaaga aattgattat tgcgacgaag ctttacaaga
    39901 ggctttattc tttgctcgca agaaaggggc cgtagtcgcg gttgctgccg gaaatgataa
    39961 tgtttgggaa cactataatg ctcctgcagt gtgcaatgga acgatcaagg ttgcttccac
    40021 cggaccagaa ggcttgcgtt cgtatttttc aaattatggg cccagtataa cccttgcagc
    40081 accgggaggt gataagcgtt atggcacttc gggaggtatt ttatctactg taaacccggg
    40141 aggtggatac aattcatcag gctttgattt ttatcaaggg acgagtatgg cttcgcctca
    40201 tgtagccggt gtagcagggt taattcttgc tgtcagtaat aaggctttga aacctgaaca
    40261 agttgaacaa attttatatg ttactacgca tgagtttggg caaagcaacg acacgaataa
    40321 gtcttgtgtc ggcaaaaaac cttgtggaca tggaatattg gatgcggata atgctgtgca
    40381 ggcagcatta aatgcttatg atgtcttatt ctcagcgcct aaaatgactg atttggcaac
    40441 gcatgaatgt ggcgagaata gattaaaacc tggtaaaaat atcattaatg acagtactgc
    40501 gacctggatt caagctaagt caagctgtga aactggtgca agctaccaga atccgcgtat
    40561 tgaaaaagcc aaggatggaa agatttatgc ttattatggt tccgcaagct atgaattgga
    40621 tcaatcagct tataaagaat gtcgtgtcgt tggatatgat ggcgtaggtt gttatttata
    40681 atatcaaatg aaactcgatt ctcagtttta agacgttttt gatgagggaa gcattcagag
    40741 ccaatttact ggttacttat ggaatccttc tctcatcaaa ttaaaatctc tgcattcgct
    40801 caggtattaa aattttaaca aagacatcag aaaattactc aagccaatta tttaatatat
    40861 caacaatttt tttggcttgt tcagtactcg aaatattgaa tttggatttt tgccaatatt
    40921 gattgttacg tttctgataa cgccgtatag aatattttac aggaccgtaa tcattttttg
    40981 tgttatccca atcctggtat ttaaacataa tagtagtcca cgctcctttg gatagtattt
    41041 gtttatcgag ttccctggtt gtttccactc cattttcgtt aaaagcaatt gttatatcat
    41101 ctatggtttc agccatttgt tatacttcct caattatttc cgatggattg taatttgccg
    41161 tcgacaaaag tcaaggaaaa ggctggttga tatgggcctg gcttataaat ccagatttgt
    41221 ggttttgtct ctgtaggaac cacttggtta atgtacgtgt tattgaccag gtttggactg
    41281 ccgcaagcgc cataaacttt agtggcagga tctccgattt gaatgcttgc gttgccacac
    41341 aaggaaaaag cgttagtatt gccgccattc actcgaatat cctggacttt attatttaga
    41401 atatcaactt ccaattgtac gccactacta cctgtaggta tattccatac gccataaaaa
    41461 gcggtacctt ccggtacatt taatgatcct gaatacaggc catcggagtt tcccagatta
    41521 ttataaatta gttgttgtac agggattttc tgcaaaatag gctgattgga ctcttgttta
    41581 ctcagtggtt gcccgcacgc agcaatgacc tgctccatgg acataccgat attgatataa
    41641 ccatgattct gagggcaata caaagattgt gcgctcatta aacggaatgg cataattagc
    41701 aataataaac tcataagtaa tatcgatttc atagcttttt ccattaatcc gtatcaacta
    41761 atagtatagt tctgtttgtt tatttttgct tttataataa cttccagggc aaagaagcgc
    41821 ttagaaacag tgtaataatt tttaatatca ccatgataat aaagggtgag aaatcaaaac
    41881 ctgaaatgtc cgggactata attctgccta gtcgtaaa
//
LOCUS       DAXLDT010000008        41196 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXLDT010000008 DAXLDT010000000
VERSION     DAXLDT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 41196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41196
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.008"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(65..823)
                     /locus_tag="ACF2SP_000361"
     CDS             complement(65..823)
                     /locus_tag="ACF2SP_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358172.1"
                     /translation="MNLVQRMNPFFWLVILAIYSTSSMALSVELIETSPKSPVILNQD
                     EALYVLIRYKSDQPLRFQAIGENLRQKIMDSARFNPSQAYPAGEGEAIAWVAYYNTTE
                     IDSITVTIYDANWRALQTKSIPVSAVWQNENGRNNQAAAPWVQRLNQQQQSSVFIQSQ
                     TPISSGDAQFVQLLFLLIPLYWFLQIIVIFNWTGRWKKLACFPLLFSIPLLLYTLYAL
                     CAGSNLWPLMMLFMTPFILVFLLIIIGYKKVYSR"
     gene            complement(957..1973)
                     /gene="truD"
                     /locus_tag="ACF2SP_000362"
     CDS             complement(957..1973)
                     /gene="truD"
                     /locus_tag="ACF2SP_000362"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFD2358173.1"
                     /translation="MYSLDWPRAYGIPNSIATFKSLPEDFQVNELFEGQFSGEGEHIV
                     LKIEKRGLTTEEVVKSLARLINKPVKLISHAGLKDKQALTTQWLSVHAPGEIIDGIET
                     LEAPGWRILECTRHNKKLKPGFLSGNHFIITLRNVSDESDLIHRIEQIKFKGVPNYFG
                     EQRFGRDGGNLIKAEEILVQGRKVKDRFLKGMYFSAARSWLYNLILSRRVKESSWNLP
                     LLGDVIQLVGSNSIFVNDKSMDEQLLQRIGEKDVSPASPLPGRSKNLVKGTALQIIND
                     VYEEWSAWLDGLEKNGLEEAWRANILYAEQIEYRINQGTVELSFVLPAGAYATVVLRE
                     LVQY"
     gene            2266..3249
                     /locus_tag="ACF2SP_000363"
     CDS             2266..3249
                     /locus_tag="ACF2SP_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFD2358174.1"
                     /translation="MIVNSSFRPLWWLSNRHAQTLYPTLVKKRLKPRIDFHERLELPD
                     GDFIDLAWSVNELNKNTPLVILLHGLGGGINSIYVSGLMQAFADAGFRCVLMHFRGAS
                     EEPNRILRTYHSGDTADFAYFLEILAKREPATKKAVVGISLGGNVLLKWLGETASSLW
                     VDAAVAVSVPFQLNSVADRMNQGFSRLYQAYLLSRLRRIFLKKLNHLNNSPFSKNDLL
                     SLKTFWDFDEQITAPINGFKSAHEYYQKASSRQYLKNISTPTLIIHAQDDPFMTPDVI
                     PTLEELSSQTTLELSEYGGHVGFIAGTGKSSSQPVYWLEQRIPEFLLDYLS"
     gene            complement(3274..3669)
                     /locus_tag="ACF2SP_000364"
     CDS             complement(3274..3669)
                     /locus_tag="ACF2SP_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358175.1"
                     /translation="MQNEKEVKELIQSDKKKRVTWNDNQTKGQIEEKFLKIGTASTLY
                     KNKLDENIAQLMEEGYSEIEATSITGKSVTPRGKVIYNNPVFYKSKESIDIEKANRYN
                     FFSIRNIGLGVVAIAAAAATVGLVVSKNS"
     gene            complement(3904..6111)
                     /locus_tag="ACF2SP_000365"
     CDS             complement(3904..6111)
                     /locus_tag="ACF2SP_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HFD2358176.1"
                     /translation="MAHNHHDGHSHVHNEHVCCHKKSNTHKPQITSEGTIFYTCPMHP
                     EVRQATPGICPLCGMALEPEAISIDEATNPEYIDMKHRFWLALILTLPVVVLEMAGHG
                     FQHFVSTGISNWIQFLFATPVVLWCGWPFFLRGWQSLKARQLNMFTLIAMGIGVAWVY
                     SVVAVFFPGLFPVAFRSQEGVVSVYFEAAAVITTLVLLGQVLELKAREQTGSAIRALL
                     KLAPENAHRIKEGGSEEEITLDQVEVGDLLRVRPGEKIPVDGEIQEGHSFVDESMVTG
                     EPMPVAKEIGDKVIGATINQTGSFIMKALHVGADTMLARIVQMVSDAQRSRAPIQRVA
                     DTVSAWFVPTVILIAVLSFIIWGLLGPQPSLGYGLIAAVSVLIIACPCALGLATPMSI
                     MVGVGKGAQNGVLIKNAEALERMEKVNTLIVDKTGTLTEGHPKLTHIETSEDWDERQA
                     LLLAASLEYHSEHPLASAIVKAAQEMQITFSSVQNFEAPTGSGVVGRVNSHHVAIGNA
                     KLMQEYGSDDAALFEKADKFRAKGSSVMFMAVDGKTVAFLVVEDPIKSTTAETIHALQ
                     KKDIEIYMLTGDSKKTAEAVAATLGIKNVIAEIMPEDKGRIVSELKENGLIVAMAGDG
                     VNDAPALAKADVGIAMGTGTDVAIESAGVTLLHGDLRGIAKARYLSEYTMNNIRQNLF
                     FAFIYNMLGVPIAAGIFYPLTGLLLNPVIAAAAMALSSVSVIVNALRLRRVTL"
     gene            6320..7180
                     /locus_tag="ACF2SP_000366"
     CDS             6320..7180
                     /locus_tag="ACF2SP_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358177.1"
                     /translation="MKKILITLIGFLFMNTYHHLLYADNSHHHTVKDLSTSSSKLENT
                     PTIQLEVQKIIDKKDKKLVFVKLTDIKSNKPITFNDLTEIHTKKFHLLIIDNHLIDYS
                     HVHPAETTTPGIYQFEWQPNFKNATYRAWADLLPTNTNKQEYATADLTSPQTVTRTTN
                     PIHHRTVLESTVNGYQFKLTFNKMPLLAGKPAMGEIDVTDSKGHPVRSLEPIMGAYAH
                     IVGFNEDFKTVIHVHPMGKEPANSTERGGPQLQFHIEPNKAGFIKMFAQVQIDGKVLY
                     APFAFTVKNA"
     gene            complement(7274..8548)
                     /locus_tag="ACF2SP_000367"
     CDS             complement(7274..8548)
                     /locus_tag="ACF2SP_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F390 synthetase-related protein"
                     /protein_id="HFD2358178.1"
                     /translation="MLARILFHYFNTLYLNKTITTPEKLKTHQAKQFEKLIKNCLHQS
                     AFYRDYLKTPLEQWPIVNKKIMMEHFDDINTVNVKKTEALEIALRAEHTRDFSPMINN
                     IAVGLSSGTSGSRGLFLVSEQERDAWAGIILAKAMPNGLRSRERIAFFLRANNQLYIT
                     LNKSRTIQFHFFDLFADFDTHIQRLNAIQPTIISAPASVLLMLASHQDILKITPKKIF
                     SVAEVLEPKEEEILTKAFGCTISQIYQCTEGFLAISERKTNRLLMNEEFLIIEKEWLD
                     EERFVPIITDLYRSTQPIIRYRLDDVLVTERSNGVFTELKNIEGRLGDVCYGKRGQEI
                     VPIFADVIRQHMISSGVSFEDYLICQQTLTQFTIQISPEISNKNQFIEHLNQLFIYKN
                     CELPQWDWELYDKNQPGVKRRRVRSLYCPKTE"
     gene            complement(8542..9378)
                     /locus_tag="ACF2SP_000368"
     CDS             complement(8542..9378)
                     /locus_tag="ACF2SP_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD2358179.1"
                     /translation="MVLFMNIEYQLFEAGYCKHCERMTLKNGKIRQREYPSFCTLIKH
                     PIHGYILFDTGYSDRFTCLTQKFPASIYRHLTPMTLVKSLKEQLLEIHIKPEDINYIV
                     ISHFHADHIGGLCDFPNAHFICHSDAIDDIQHTKGLKALLKGFLPGLLPEDFFERLLP
                     LQKTIQLDEHLLPFSDGFDVLGDNSLIAIPLPGHAKGQIGLYFKAPGDIFLVADSCWH
                     QETFKELIYPSALTYLIHHDKRAYLKTIQKLHELYQRNKTIEIIPSHCQQLRSRIGNP
                     LC"
     gene            complement(9363..10343)
                     /locus_tag="ACF2SP_000369"
     CDS             complement(9363..10343)
                     /locus_tag="ACF2SP_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFD2358180.1"
                     /translation="MVSVVTGATGCLGLNLTRRLIKEGHAVIALGRNLQLGEIISQMG
                     ATFVPLDLKDLESLSLISQNADFIFHCAALSSPWGKYKDFYQANVIGTHNVVQATPSQ
                     ARLVYVSTPSIYFDFTEKHNIKENALLPAKPVNYYVQTKLIAETIVDKAQLQRGLDVI
                     TIRPRGIFGPYDRAIFPRLLKAERQGVLPIIGSGNHLIDITFVENVVESLILAALADK
                     CYSGKKYNITNDEPRTFIDIISRMFSALNKPLKTRSIPYNQARFAAKFLEFLHRVLYL
                     KTEPKITEYGVGVLAFGQTLNIEEAKKDLKYKPIYSIDEGIMKFAEWYCS"
     gene            complement(10345..11328)
                     /locus_tag="ACF2SP_000370"
     CDS             complement(10345..11328)
                     /locus_tag="ACF2SP_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFD2358181.1"
                     /translation="MSSAKIIGMGKYLPANIVFSSDLDKKLNLPEGSVQKKSGLVSRH
                     FARVNETTSYMASQAALHAVEQAGITLAEIDAIIGACGAGEQPIPCTAVLVQKQLGLD
                     NSGIACFDINSTCLSFLTALDAASYMLSGGRFNRILIVSSDIPSFGLDWSDLETCTIF
                     GDGAAACVVERSNDSSRILSAHMKTYSLGAAFCEVQAGGTLMPPASEYDKKYGLFKMD
                     GKRVFKLASQMIEPMQEELFAKASITLNDVDWVVPHQASLLAMHHIRKKLGIPGEKFV
                     DIYTTHGNQMAASLPSALCHLIHNHNLERGQLVYLLGTGAGLSAAGMILEY"
     gene            11478..12644
                     /locus_tag="ACF2SP_000371"
     CDS             11478..12644
                     /locus_tag="ACF2SP_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD2358182.1"
                     /translation="MKILTVLDSYPPDLNGGAYFTHRLAKFLQAKGHEVLVICPSRSL
                     KQGYTSYEGVNLYGVRSWPALGYKNFRVCWPFFIKKGILKAITDFNPDVVHLQGKFFL
                     GGICYRACRKKDIPLIATNHFMPENFFHYTHLPRYFEKWFHRTTWNIVIDMLNHVKIV
                     TTPTHTAANLLKEVHVQKEIHVVSCGVDLQKFQPKQNANLIRQRYKIPDKPILLYAGR
                     LDKEKNLSIAIKAFYKTRQSIDAHFVLTGCGAELQRLKKLVQTLNLTEHVTFTGYLSD
                     AEYPLVYSLANCFVNPGTAELQSIVALEAIASGLPLIAAKAMALPELVKEGVNGYLFD
                     PNDVETLSCYMVKILSDRTLSEQMGRESRKLSQEHDIKRTIEQYETLYQSMVSL"
     gene            12641..13297
                     /locus_tag="ACF2SP_000372"
     CDS             12641..13297
                     /locus_tag="ACF2SP_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HFD2358183.1"
                     /translation="MKTVANRYHVLCQEKVFIRSLLSGFFLLFISFLIMNFAGKYASL
                     MAGNPVNDLILDNLPLRDVTVFHVNAAIIFWTIFTIYTLLNPALIPFITKSAALFILI
                     RCAFISMTHLGAPPNQLSIPDNFSSLVLFDGDLFFSGHVGGPFLMMLIFWNKTTLRYI
                     CLAAAVFFAYTVLIGHIHYSIDVFAAPFITYGIFHISQFLFASDYQRLKSELTSSTGN
                     "
     gene            complement(13477..16377)
                     /locus_tag="ACF2SP_000373"
     CDS             complement(13477..16377)
                     /locus_tag="ACF2SP_000373"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFD2358184.1"
                     /translation="MIPRLTSSTATPRKIRLFLQQLEKIPDFHGEIDEALSSRIVLST
                     DNSVYQIIPETIIFPKSESDISHIFKLASSEQFRDLTFTPRGGGTGTNGQSLNAGIII
                     DCSRFMNHIVEINPYEDWVLVEPGVVLDQLNEYLKPYNKFFAPALSPSNRATIGGMVN
                     TDASGKGSRLYGKTSQHILALTAVYLGGEVHTSEEIDVSLLDNMKKMPGQVGQFYKQL
                     DKTVQEHRETIDRQLPKLHRFVTGYNLAKIYSNDDKRFNANYILAGSEGTLAYVTKIK
                     LKLTSIPKYKCLFVVLYPSFSDALEHAKHLLAFDPEAIETMDSTILKLAKTDIIYDSL
                     KPFLNSGSNSQVDAINLIEFVANDEKELLHHAQLLKKNLKGTDFHFVTNNEDIINLWE
                     LRKRSVGLLGKVKGVRKPISGVEDTVVPPEYLADYVKEFRELLDSHGVSYGMFGHVDV
                     GCLHVRPELNLMDAKDREIYYEIIEKVAQLAHKYGGVLWGEHGKGFRSEFVPVFFGEE
                     LYNELRKIKSSCDPFNQLNPGKIAVPIGSHDSLASIRSEKTRADVDNQINYQYRDSYQ
                     LPLNCNGNGACFDYKLQDVMCPSYKISRDRIHSPKGRASLVREWLRQLSALAGKRELT
                     WPLKKRLNTYKRNHGQYDFSHEVYTALEGCLGCQACRSQCSVNINVPSFKAKFLQTYH
                     TRYSRSIKDYLIAYSERVGYFQSLFPELTNFCLSNKLVKYCLHKYTGLVDLPLFSSPN
                     LKSLLKANNIPWLDLNHLPVSEKTVIYIQDWVTSFYEAELVLKACQLIKKLGFQVYVL
                     PWFENGKTLHDKGMLQAFAKITRKNALMLKTLSDHGFTMIGLDPAMVLTYRYEYSEYL
                     HENYNSKILLIQEWLSQILGNENINELPIKANEKLKQYLLLSHCTEQANCSEALIQWQ
                     KVFQFFGLTLVAEKTGCCGMAGAYGHESIHQLNSHGLFDLSWKNYFEVYLIL"
     gene            16532..17071
                     /locus_tag="ACF2SP_000374"
     CDS             16532..17071
                     /locus_tag="ACF2SP_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HFD2358185.1"
                     /translation="MNTITEDSLTEEQKYMVQVWENHTHYEFDTKNVADTMKTMEDNP
                     YVNNIPTIAGGFGQEEVKQFYSQLFIPQKPDDTEVELISRTVGTHQIVDELIFKFTHN
                     IRMDWILNGIEPTGKRVEIPLIAIVRFHNRKIISEHIYWDQASVLVQLGLLNSDKLPV
                     HGVEATNKIRELISRSLTK"
     gene            17125..17973
                     /locus_tag="ACF2SP_000375"
     CDS             17125..17973
                     /locus_tag="ACF2SP_000375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358186.1"
                     /translation="MIKDYLIETMNQDEVNLAISWATREGWNPGIHDATCFYQTDPHG
                     FFAGKLNGKTIAIGSAVIYDEFFAFCGFYIVDKPYREQGFGLELTKARLAYIGNRNAG
                     IDGVINMLDKYARIGYQLAHNNARYCLNKTIKPISSEPHIVPLNQIDFSQLSSYDRLH
                     FPAPRPQFLKCWISQPQSLALAYLIDGQIKGYGVIRTCHKGFKIGPLFADTPEIAHQL
                     FTNLVPYAKGQLIFLDIPENNPLAIELVSQHQMEKVFATARMYLKGEPQVKKESIYGI
                     TTFELG"
     gene            17977..18867
                     /locus_tag="ACF2SP_000376"
     CDS             17977..18867
                     /locus_tag="ACF2SP_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFD2358187.1"
                     /translation="MTRDLTGYGSEQINLTWPNKAKIAINFVINYEEGAELTPLNGDT
                     KAEFYGSDFPFVPKGNAERNYGIESIYEYGSRVGIWRLIRLFDKHSIPLTFFVTGYAL
                     ILNPEFSSYLASHNHEVAGHGWRWIDYSKESKATEKKHISRCIDTLQELTGQRPLGWY
                     TGRRSPFTRDLLTQIGGFVYDSDSYADELPYFINKHLVIPYSLDCNDFRFTISPGFVT
                     AQDFYQRLKNTFDYLYQENRLAIMTIGLHPRLSGKPDRCHALNEFLSYIRQFQNIWIA
                     KRIDIALYWLTKHTQIKQDL"
     gene            19083..20297
                     /locus_tag="ACF2SP_000377"
     CDS             19083..20297
                     /locus_tag="ACF2SP_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358188.1"
                     /translation="MSHLLDLSILKKNRDFTLLYLGQFISFIGTMITSVALPYQIYHL
                     TESTMMVGLLSLAQLLPLLITALLGGVFADRYSRRGLLIISELLLAVGCLFLAFNSKL
                     VNPSVSLIFIIASLMSAITGLHRPAFDSVIQQIVKPEDYKKVGALGSFKFSFCMIIGP
                     AAAGLIIAQYGIVITYLIDLLTFIISLLNLSLMHTIPKPLAKEHPSIIVSLKEGIGFA
                     LRRQELMGSYFVDFFAMVFAMPNALLPAIAQQFGGAKTLGLLYAAPAIGSLIISFVSG
                     WTAKITHDGKAIAIAAALWGVAIIGFGLTTSLWLSLLFLALAGAFDAISGIFRSSLWN
                     HTIPQELRGRLSGIEMISYLSGPRLGDTRAGFVAAGLGIPAALISGGILCIIGVTVCC
                     YALPKFWNYHSK"
     gene            complement(20310..21626)
                     /locus_tag="ACF2SP_000378"
     CDS             complement(20310..21626)
                     /locus_tag="ACF2SP_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1639 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358189.1"
                     /translation="MAGIYLKYYVPVNQIGENMMINPIEYHPKGDKVNSDYFNEYKTK
                     IYERRLSSGLEDLFGDMCGVVVQVQTGDAIPYIKELYSMTPYRYSRSYISDTHKIYCL
                     LNTKNSPAFLVLEPLDPNFKDDITRLNKMYPNSRAKFNARYVGEIYKCKDKDETRNIL
                     VSHDFNFHNPDMTENKFYCNKHIHFTRLSDFTYNCTGYTDDNIYDFDCLELGQRFYLT
                     KEQEAMLDAKDQESHDNGIKDLIKGIDHMATRILAGEREDAILEFLCLSKYYFWGAYN
                     IYDMNSSTNVNRNPHGHDIKSPAKVFTANNTPFMVNSFENLPMPTETFVRNYGRRMHH
                     IAYEVKDGDHSSGKKNIDFVVETLRDKLNIEFLAKVFGACEDSPDLKQIFSKHSIYSI
                     LITEYVERCHNFDGFFTKENVAALTEAASLDETTKQQHKKASIIGD"
     gene            21717..22274
                     /locus_tag="ACF2SP_000379"
     CDS             21717..22274
                     /locus_tag="ACF2SP_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HFD2358190.1"
                     /translation="MEALIVSLGVITLAEIGDKTQLLAFLLAAQFKKPLPIILGILTA
                     TLINHSLAGLIGIWITTLLKPNVLRWILGISFILMAIWTLIPDEIEQDEKKISKYLGV
                     FGATFITFFLSETGDKTQIATIALAAHYSSPILVIAGSTLGMLLADLPAVYFGNLFSH
                     KIPMKIIHAVAACAFLIIGLITILF"
     gene            complement(22292..24262)
                     /locus_tag="ACF2SP_000380"
     CDS             complement(22292..24262)
                     /locus_tag="ACF2SP_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFD2358191.1"
                     /translation="MKGLCLGLIICLVVFHDGWAGKRLPELNDLYRIQDISEPDLSPD
                     GKWVAYTVSQLNAKDNISTTEVWKVSVDSKSSQRITFAEDSSSSLPKWSPNGKWLAYL
                     SDGGKEGTTQIWIASVQDNKEHQLTHIDGEVSDFTWSPDSNRIAFIAQKSDPSNEEEQ
                     PIVVDRYQFKVDGVGYLGKGRDHLYLVELKSKAITQLTDGNHDEYLPAWSPDGKYIAF
                     VTKRGKDPDRHFNYDIYLIEPKSGAKEHQLTHFPGSDMNPDYDSNLSWSPDSSKIAYL
                     RSNEHQWTYYSPNQLAIIDINTKDEKIIAPRDNWYYKPKWSQDGKFIYALIEESRNTY
                     LNRIDIHSGQIKKLTGGLRYDMDYSLAKGKIILLTTDDTHPSELFILNSGLIPLTQHN
                     QKLLDEVKFQPAEDIQCNSFDGTRVDGLLMKPAEYQPGILFPGFVYLHGGPVYQFSHE
                     FNFDMQWIAANNYAVIAPNPRGSSGRGFHYAKAIFADWGNLDVKDILASVDYVIGKGI
                     VDPNRLGIGGWSYGGMLTNYVIATDSRFKAAISGAGAANILAGYGVDQYTPEYELELG
                     KPWTNPELYLKLSYPFLKANNIKTPTLFLCSGLDFNVPCVGSEQLYQALKSLDVPTQL
                     VIYPNEYHTLEKPSFVIDRLKRYTNWLDTYVK"
     gene            complement(24472..25725)
                     /gene="sidB"
                     /locus_tag="ACF2SP_000381"
                     /pseudo
     CDS             complement(24472..25725)
                     /gene="sidB"
                     /locus_tag="ACF2SP_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidB"
     gene            complement(26105..27373)
                     /gene="thrC"
                     /locus_tag="ACF2SP_000382"
     CDS             complement(26105..27373)
                     /gene="thrC"
                     /locus_tag="ACF2SP_000382"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFD2358192.1"
                     /translation="MKIRIKSTRGQCVSTIDEAILSGLAGDGGLFVPDRFPNLTIKKF
                     YEYGNLIDFSAHLLSPFFSDSKIDVNNAFFQKFLTFSFPLHHLFKKSYVLELFHGPTL
                     SFKDLGTQFFIECLLHLIENKSAKVLVATSGDTGAAIAHALHGKRDLEGIILFPKDKL
                     TFRQQSQITCWGENIRALAVNGSFDQCQQLVKLVFAKREHKGVLTTANSMNIARLLPQ
                     IIFYAFTSIQLALRHNHSVNFIVPGGNLGNVTACYWAKMLGFPIEQILIANNTNSALG
                     EFLLTGAYQAKSVTKTLATAMDVGEPSNLERLFILFNNYPECRKQMNAESVSDEQIKQ
                     AILHCYNKYHYIICPHAATAYHRLSTINQDKSWVIVAPGHPAKFEEVLEPLLNKEIPV
                     PEQLKVFLGRKQHYSIIEPNYHSLYHFLVE"
     gene            complement(27546..28310)
                     /locus_tag="ACF2SP_000383"
     CDS             complement(27546..28310)
                     /locus_tag="ACF2SP_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFD2358193.1"
                     /translation="MYLGFPVQSILLFLFFSFTPLFVLAAEKTPLNFFLRPDTYFGFS
                     VIRDTAHYNFKRDETSQELNFTDFDHFDWHGSGLGGEIYLGYEKFFRTHYYIGLEGFF
                     NRSSNEGKIYFFDSNELYSRILKGSFKQKWQSGLAIHPGIYLPSVGIIYGRVGWIVSD
                     VNLSGSITQSGPFGSFSGRFSNTKKKNGVQLGTGLELELTKNWRCRVEWDWNRLQDFI
                     FDSQGRDSNNHRYQTTRIAKHPILEQFKLGLNWRFA"
     gene            complement(28611..30635)
                     /locus_tag="ACF2SP_000384"
     CDS             complement(28611..30635)
                     /locus_tag="ACF2SP_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HFD2358194.1"
                     /translation="MPIPLLNFKHFQNDNSVFRSTTHHNHMVSRGTFPGNPKTWFAKV
                     CSDPASARNEVLAQEFFRLIIPHQPETLIAKDEAQNNYYVYSEEVEGFKNLPYGQGKS
                     FEDGTFTGLGQAVFVSVFLQEVDLKNGNIGLDKDGRVIKIDGDQCIVSMLGVDQRFDL
                     TPKVIDSLPRPVDFATNNWLDYKGGYFSLQRILQPTITNNEAFRSEVNQAMLKICLIP
                     DEYIERFVSAYIPDGHKPDKQGYIDLIKSRRDVLRLSAVKNESFRNYLSEPAAQEDAK
                     HFMEHMRSFKSGGEPVLASQQERSQLTIDLALQNLKLETAFIQQSEKVVTVKNAVEKE
                     AFADQENELAMVFRFQMEMKKQAQLSDSSFISIISRSSQASAEKTAYQNFLNKSVNPA
                     MEKCFAALVRNDLKQLEQVLAEFPNNEKWSAFNLQESEVAKAQMDAIKRLIEERVFIR
                     GKILPVLEQCKDAIDKKDIPAALQALSAFPPDKDMSKTTSISSGLKGQIIEMKQDAIE
                     SLNVIAQQSPISTPVIDDAEKVESGYSPLLVEITKQIEALESEHCEDLPKLEMHLTTV
                     NDCLDTVEFLISERIKQHGASEDPLELSSLEELRSRLQKIKKTDENALNAYPKDNAEI
                     TQTETQPSQVLPQTSNNTNPAKRTIKSDYDVDSENKVTKIGEDRPGFKPE"
     gene            complement(30782..31357)
                     /locus_tag="ACF2SP_000385"
     CDS             complement(30782..31357)
                     /locus_tag="ACF2SP_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFD2358195.1"
                     /translation="MNVYIKSSLWVLIFFVNTLTYASKLPEWEIVPAQSEITFTGTQN
                     GAPVTGRFKKFTGQIFADPSNYKTGSIDITIDMNSIAAPFEDIVTTLASPDWFNVKTF
                     PNAEFKASKFNKLDDKTYEAEGILTIRDKSAPVTLKFVVDQISNDQALVEGSTTIKRS
                     TFGVGQGEWASTDEIQDEVIVRFKITATRKK"
     gene            complement(31357..31887)
                     /locus_tag="ACF2SP_000386"
     CDS             complement(31357..31887)
                     /locus_tag="ACF2SP_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFD2358196.1"
                     /translation="MQLRNTSTSFGLVAIFFHWIIAALIIGLLGVGLYMVRIPISLEK
                     LKLYGWHKEYGFLVLFLAFFRLIWRLTNQLPELAIPLLEKIAARSMHWAFYFFMFAMP
                     FSGWLITSAAGLPASFFGLFVLPDLVAPDENRRILFQLIHEWLGYALIMAIILHAAAA
                     LKHHFINKDDILRRML"
     gene            complement(31891..32484)
                     /locus_tag="ACF2SP_000387"
     CDS             complement(31891..32484)
                     /locus_tag="ACF2SP_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFD2358197.1"
                     /translation="MKRIYRHFIASILAVFALTSYVYAEPQTLTLDNQHTYVLWKVKH
                     LGFSTQAGKWYASGQLVLDKDNPQQSKVNVTIKVDDIVTGIPELDKHLKGKLFFDTKQ
                     FPTATFASNRVEVTGKNKAKVYGMLTLHGVTKPIILNVILNKAGINLLNDRETAGFSA
                     TTSLKRSDYGIKALIPEVGDDVEIEIEAEAYLDKKYG"
     gene            complement(32512..32769)
                     /locus_tag="ACF2SP_000388"
     CDS             complement(32512..32769)
                     /locus_tag="ACF2SP_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HFD2358198.1"
                     /translation="MSMKDKLIQSAISAFLVLIASNTAIAEDLSKEKCYGVVKKGMND
                     CSTATSDCAGSSTRDSQKDAFILLPKGICEKLVGGSLDMGK"
     gene            complement(32891..33787)
                     /gene="iolE"
                     /locus_tag="ACF2SP_000389"
     CDS             complement(32891..33787)
                     /gene="iolE"
                     /locus_tag="ACF2SP_000389"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFD2358199.1"
                     /translation="MEVKMSIQLGIAPINWCNDDDPNLGKDISFEQCINEMALAGYSG
                     TELGNKYPRDATTLKAALEQRGLQLSSSWFSTYFTEANAFEKTVSRFLEHLSFMRALG
                     ASYINVCECGHAIQGTDLPILSAHIPQFTQEQWSLLIQGLHTLGRIAYDFNMKIVYHY
                     HAGTGVFWEKDIDFLMTNTSPKLLSLLLDTGHAVFSGIDPVNLINKYGDRILYVHLKD
                     IRPEVLRRVEMERLSFMDAVRRGVFTVPGDGAIDFIPIWAGLQKHRYNGWMIVEAEQD
                     PEKAHPLEYAKKAYDYLHSFIH"
     gene            complement(33790..35661)
                     /gene="iolD"
                     /locus_tag="ACF2SP_000390"
     CDS             complement(33790..35661)
                     /gene="iolD"
                     /locus_tag="ACF2SP_000390"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFD2358200.1"
                     /translation="MKIKLTMAQALLRFLANQYVVLDNNEYRFINGIFGIFGHGNVTG
                     LGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAA
                     TATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
                     LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAML
                     AEAIRHIRKAKQPLIIAGGGVHYSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMN
                     VGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAM
                     KMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLICHYQNEWAQELDKLTQSYSTEQA
                     GLHQTAVLGLLNQYVTEEDVIISAAGSLPGDLHRIWQSKRAKDYHLEYAYSCMGYEVA
                     AGLGVRIAKENQEGEVYVLVGDGSFIMMHSELLTAIQERKKITILIFDNHGFQCIRNL
                     QEGNGSLGFGNEFRYREPTTNALSGDYLPIDFCKYAEGLGAATYFVKSYEQFWTALDS
                     AKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMQEQIETVRQY"
     gene            complement(35700..37586)
                     /gene="iolC"
                     /locus_tag="ACF2SP_000391"
     CDS             complement(35700..37586)
                     /gene="iolC"
                     /locus_tag="ACF2SP_000391"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFD2358201.1"
                     /translation="MKQFPGFYFDKNRAIDLICMGRVAVDLYAEQIGLDLKDVASFKK
                     YLGGCAGNIAVGAARLGLKSQMFSCVGSDELGKFLKEELEREGVNIELLSETDNHLTG
                     LVLLGIKPPSNFPLLFYRNDCADMQIKSDQISFTDLKNAKALLITGTGLSTESVKNTT
                     LEVVHLARKAETTIVFDLDYRPVLWRLTVIGNGESRYCQNEYVTQVYQQVLPLCDLIV
                     GTEEEICIAGGSNDIEVSLINIRQRTDAPIVVKLGEKGARVHFGTDKGLLQAKSFPVE
                     VLNVLGAGDGFMSGLLSGLLQGKSWEQAVTYANACGALVVTRHGCAPAIPYKEELDYF
                     IQEYEHDPEIWSSSQLTQLHEKLSKNQKTQLLIKNPCGFADGLNSIVTMQDSPTAMSF
                     SSLKLNKGQSHQFNSSYEFAALLMTGKVVFQYGSYSQEVERTDYFSQSPYVLHCSKNE
                     VSSVIALTDCEILLMETENENLFSPILFDASNMLELDNRGEGVLENTSYRLVRTVFDK
                     RNRPESNLVVGEIITFQGRWSSYPSHYHQQPEIYHYRFSEPQGYAFGENGKEVMRIEH
                     YDTYQIANNQEHAHCAAPGYALYTLWFIRHLNNNAYTLPTFIKDHEWTRTSRANLRAW
                     QQKQ"
     gene            complement(37594..38598)
                     /gene="iolG"
                     /locus_tag="ACF2SP_000392"
     CDS             complement(37594..38598)
                     /gene="iolG"
                     /locus_tag="ACF2SP_000392"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFD2358202.1"
                     /translation="MKKKTCRIGIVGAGRIGRLHAENILFHLPQFELVAIADPQIDRN
                     WAEKLGIAIVSNQSEDVLCHPELDAILIASPSHLHLEQIITASIHEKAVFCEKPIGLN
                     EEEIEKTLNIIKNNNTLLQLGFNRRFDPSFASIQKRVQFGEIGTPHILRITSRDPACP
                     SKEYCATSGGIFMDMSIHDFDMARFLMQSEVVEVYATGAVLINPDFEQFDDVDTAIIQ
                     LRFANGAFGVIDNSRQAIYGYDQRIEVFGSAGMLLAENQLEHTVSRYSANGTDGAKPQ
                     YFFLERYHQAYLAELNAFYTAWNDNQTSPVSGLDGLQALRIAKAAKQSLETHLPVVLS
                     "
     gene            complement(38601..40016)
                     /locus_tag="ACF2SP_000393"
     CDS             complement(38601..40016)
                     /locus_tag="ACF2SP_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFD2358203.1"
                     /translation="MNKGMNAFVFLIAVIAGFGGFLFGFDSSVIADVKDQVISQLALT
                     DWQWAQVVSSSLLGCILGIPISGLVADKLSRRSLLKVVALGFILGSSLCALAPGFVSI
                     LLGRFIIGICIGIASYIAPLFIAEIAPPHQRGTLVLINGLTITFGQAIAYLIGYFLHD
                     YSLMSWRYLFWIGSLPALVLFSGMYFVPHSPRWIMMKYGADATLKTLKQIRPVGYNIQ
                     QELTEISNNLVNPAPAYIQMFKKPIVFVLLLGVGLGIFQQFSGINALMYYGPVVFESF
                     GFSPVKNAILATFFLGLVNFIFTVVTLFYVDKLGRRFLLISGTLIASISLYLVSLLSS
                     NSLLVNKFWILGGLSFYVMGYCISLGSLFWVLISEIYPLSVRGLAMSIATVFQWGANF
                     VVSLLFLPIYQFSGQTVTFAMFATFCLLACWFIYYFVPETTSVSLEKLEDNLSSGKKI
                     RDIGQPVSKNIKIAKFNLIMD"
     gene            complement(40468..>41196)
                     /locus_tag="ACF2SP_000394"
     CDS             complement(40468..>41196)
                     /locus_tag="ACF2SP_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1654 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358204.1"
                     /translation="LIQNKTFEEQQIGDPVANFKQFHIFYEKKLSTKSYLNSAKFMNH
                     EYLLGEGQVARQQEQALFEQFENISISEIQQIDRSLENMLSQLSLAAEPPNPNSISGV
                     DRVIFNPIFVNVTPSDSSYRGMAMQVLNLAKSYIQNQKISDAIATYQQAIKILNTIKS
                     PIESDLQIVQELTQHIRYLQNKPSSTKDQFSSWYKEAQAPVIATASEMGFFNYRPQEA
                     FQVSDSFHSPNEMDVEYCDGNLRP"
BASE COUNT        12721 a         8392 c         7022 g        13061 t
ORIGIN      
        1 aaaaattcaa atttgataat agattaaact tttatactca agataagcat tggatgcgcc
       61 ttaattatct ggaatagact tttttatatc caataatgat caataaaaat accaaaataa
      121 atggtgtcat aaataacatc atcaaaggcc ataaattaga acctgcacat aacgcataaa
      181 gcgtatataa cagcaaaggt atgctaaata ataaagggaa acacgccaat tttttccatc
      241 tccccgtcca attaaaaata acaataattt gcaaaaacca atacaaagga attagtaaaa
      301 aaaggagttg aacaaactgg gcatcaccag aagaaatggg agtttgtgat tgaataaaca
      361 cactggattg ctgttgttga ttcagtcttt gaacccaggg agctgctgct tgattgtttc
      421 taccattctc attttgccag actgcagaca caggaattga tttggtttgc aaagccctcc
      481 aattcgcatc atagattgtg acagtaatgg aatcaatttc tgtggtatta tagtaagcta
      541 cccaagcaat agcctctcct tctccggctg ggtatgcttg tgatgggtta aacctggcgc
      601 tatccattat tttttgccgc agattttccc caatagcctg gaatcgcaaa ggttggtcac
      661 tcttatatcg aataagcaca tacaaagcct catcctggtt taatataaca ggagatttag
      721 gactggtttc gataagctcc acactcaatg ccatggatga agttgaataa atagccagga
      781 tcactagcca aaaaaaaggg ttcatccttt gaaccagatt catgaatgtg tccctcaatt
      841 aaataaacat ttctctttat tatggcataa catcaaacat agtgattaat tcatcaattc
      901 aattaaaaaa taattactca acatcgaata aagtgctatt caagtgcaaa caaagcttaa
      961 tattgaacca gttcacgtaa taccactgtc gcgtaagcgc cagccggcaa gacaaacgaa
     1021 agctcaactg taccttggtt tatcctatat tcaatctgct ctgcataaag gatgtttgca
     1081 cgccaggctt cttccaaacc atttttttct aacccatcaa gccaagcaga ccattcctca
     1141 taaacgtcgt ttattatttg caaagctgtc ccttttacaa gattcttact cctaccaggc
     1201 agagggcttg ctggagaaac atctttttca ccgattcgtt gcaacaattg ctcgtccatg
     1261 cttttatcat taacaaatat actgtttgat ccaacaagct gtatgacatc ccccaataag
     1321 ggcaaattcc atgatgattc ctttacacgg cgagacagga tcaaattata tagccatgat
     1381 cgggctgcag aaaaatacat tccctttaaa aatctatcct ttactttgcg cccctgtact
     1441 agtatttcct ctgctttaat taaatttcca ccatctcgac caaaacgctg ctcaccgaaa
     1501 taatttggaa cacctttaaa tttaatttgt tcaatacgat gtattagatc actctcatca
     1561 gagacatttc ttaaagtgat tataaaatga ttgcccgata aaaaacctgg tttgagtttt
     1621 ttgttatgac gtgtacattc cagtattctc caacctggcg cttctagtgt ttctatgcca
     1681 tcgattatct ccccaggtgc atgaacactg agccattgag ttgttaatgc ctgtttatcc
     1741 tttaatccag catgacttat aagttttacg ggtttattaa ttaacctggc taatgattta
     1801 acaacttctt cggtagttaa tcctcttttt tcaattttaa gaactatgtg ctctccttca
     1861 ccactaaatt gtccttcaaa caattcattc acttgaaaat cttccggcaa cgacttaaaa
     1921 gtagctatag agtttggtat gccataggct cgtggccaat ccaaagagta catcaagatc
     1981 tccttcaaca aaaaagggtt ctaaactaaa tccacaaatt cactataagc tcacagagat
     2041 acagaactta ttagttgaga taatgcatct cttcgcaaaa tacctatctt aactatcgtt
     2101 tgtcatttga tatagggact ataaaacatt atgatgcaag attaccaaca aaaattagtt
     2161 taaattcttg attgactata atttaaatag atattttctt ttgcaatttc tgatcatggt
     2221 ctgcgttatc cagttagaat taaatttttt attttcaggt tatatatgat tgtaaacagt
     2281 tcatttaggc ccctgtggtg gttaagtaac aggcatgcgc aaaccttgta tcctacttta
     2341 gtaaaaaagc ggttaaaacc gcgaattgat tttcatgagc gactggaatt acctgatggt
     2401 gattttattg atctggcctg gtctgttaat gaattaaata aaaatacacc cttggtcata
     2461 ttactgcatg gtttgggtgg aggaattaac tccatctatg tcagtggatt aatgcaagca
     2521 ttcgccgatg ccggatttcg ttgtgtttta atgcattttc gcggagccag tgaagaaccc
     2581 aaccgtattt tacgtaccta tcattccggt gacacggctg attttgccta ttttcttgaa
     2641 attttggcaa agagagaacc tgcgacaaaa aaagcggttg tcggcatatc cttgggaggc
     2701 aacgttctac tgaaatggtt gggcgaaaca gcatcatctt tatgggtaga tgcagctgtt
     2761 gctgtttccg taccattcca attaaattcg gtggctgaca gaatgaatca gggtttttct
     2821 cgcctgtatc aagcttactt attaagtcga ctgcgccgta tttttctaaa aaaattaaat
     2881 cacttaaata attcgccatt ttcaaaaaat gatttacttt ccttaaaaac tttctgggat
     2941 tttgatgagc aaattacagc accaatcaat ggttttaaaa gtgcacatga atattaccaa
     3001 aaagcaagct ccagacaata tcttaagaat atcagcacgc caactttgat tattcatgcg
     3061 caggacgatc ctttcatgac accagatgtt atcccaacat tggaagaact atcttctcaa
     3121 accactttgg aattaagcga gtatggcggg catgtaggct ttattgccgg gactggaaaa
     3181 agctcaagcc aacctgtgta ttggcttgaa cagcgcattc ctgaattttt gctagattat
     3241 ttatcttaaa aaatcaatgg aaacgtaata cgtttagcta ttttttgata caaccagtcc
     3301 gactgttgca gcagcagctg ctatagccac tacacctaaa ccaatatttc ttattgagaa
     3361 aaaattataa cgattcgctt tttctatatc gatgctttcc ttagatttat aaaaaacagg
     3421 attattgtaa ataaccttac ccctcggtgt taccgatttt cctgttattg atgttgcttc
     3481 aatttctgaa taaccctctt ccatcaattg ggcaatattc tcatccaatt tgtttttata
     3541 aagtgttgaa gcagtgccaa ttttaagaaa tttttcctcg atctgccctt tagtctgatt
     3601 atcattccaa gtaactcttt ttttcttatc tgattgaatg agttctttca cttctttctc
     3661 attttgcata caaacctctc tataattaat caaaatttaa acaatattag caataaatac
     3721 ttaagcaaat atgaactcaa aaggtaattt aaaaattgca tctatttgga aacccagatg
     3781 aaatcgagtt gatcgatgaa attccatatc agattgtaac ttgttgttta ttaaacaatg
     3841 tatttctgat tagtatataa tcaggagtca ttacagaatg atttaataca ctttactatc
     3901 gattcacagt gtaactcgtc gcagcctcag agcattaacg ataactgata cagaagaaag
     3961 tgccatagct gctgcagcaa tcactggatt taagagcaga cctgttaatg gatagaaaat
     4021 acctgcggca atggggacac ccaacatatt ataaataaaa gcgaagaaga gattttggcg
     4081 tatgttattc attgtgtatt ctgataaata tcgagctttg gcaataccac gtaaatctcc
     4141 atgtagcaag gtaacgccag cgctctcaat agcaacatca gtgcctgtac ccatagcaat
     4201 accaacatct gcttttgcca atgcgggagc atcatttact ccatcgcctg ccatggcgac
     4261 aattaagcca ttttctttta attcgctgac aatacgaccc ttatcttcag gcattatttc
     4321 tgctatgaca ttttttatac ccagagtagc tgctacagct tctgcggttt ttttgctatc
     4381 cccagtcagc atgtaaatct caatatcctt tttctgaagt gcatgaatgg tttctgcggt
     4441 agtagattta ataggatctt ctacaactaa aaacgctact gttttaccgt cgactgccat
     4501 aaacataaca gaagaacctt ttgcacgaaa tttatcagct ttttcaaaaa gagcagcatc
     4561 gtcgcttcca tattcttgca ttaattttgc attaccgata gctacatgat gactatttac
     4621 tctacctact acaccactcc cggttggcgc ttcaaaattt tgaactgagc tgaaagttat
     4681 ttgcatttct tgtgctgctt tcacaatggc gctagccagg ggatgttcac tgtgatattc
     4741 cagagaagca gctaatagca atgcttgtct ctcatcccaa tcttcgctgg tttcaatatg
     4801 ggtaagtttc gggtgtcctt ccgtaagtgt gcccgtttta tctacgatga gcgtatttac
     4861 cttttccatt cgctcaagcg cttcagcgtt tttgattaat actccatttt gtgcgccttt
     4921 ccccacacct accatgatag acattggtgt tgccaaaccc aacgcacatg ggcaagcaat
     4981 aatgagaact gatacagcag caattaagcc ataaccaaga gatggctggg gacctaatag
     5041 cccccatata ataaaagaca ataccgctat caaaatcact gtgggcacaa accaagccga
     5101 gacagtatcc gctacacgct gaattggagc acggcttcgt tgagcgtcac tcaccatttg
     5161 aacaatacgt gccagcatgg tatctgcacc aacatgcaat gctttcataa tgaagctacc
     5221 tgtttggtta attgtagcgc caataacttt gtctccaatt tctttagcaa caggcatagg
     5281 ttcacccgtt accatggatt catctacaaa actatgtcct tcttgtattt caccgtccac
     5341 aggtattttt tcacctggcc gtacacgcag caaatcaccg acttctactt gatcgagcgt
     5401 tatttcctct tcactaccgc cttccttaat gcgatgcgca ttttctggtg ctaatttcag
     5461 taaagcacga atagcactac ctgtttgctc tcgtgccttt aattcaagca cttgccctag
     5521 cagtacaaga gtggttatca cagcagctgc ttcaaaatat acagagacta ctccttcctg
     5581 gcttcgaaac gcgacgggga ataatccggg aaaaaaaacc gccaccacac tataaaccca
     5641 cgcgacacca atacccatag caatcaaagt aaacatattc aattgacgtg ctttgagtga
     5701 ctgccaacca cgaaggaaaa aaggccaacc gcaccatagc accacgggtg ttgcgaatag
     5761 aaactgaatc caatttgaaa tacctgtcga aacaaaatgc tggaacccat gcccagccat
     5821 ttctaagact actacaggta aagtcaagat caacgccagc caaaatcggt gtttcatatc
     5881 gatgtattcc ggatttgttg cctcatcaat ggaaatagct tctggttcta atgccattcc
     5941 acaaagtggg caaatccccg gggtagcctg gcgaacttca ggatgcatag ggcaagtata
     6001 gaaaattgtc ccttctgaag taatttgtgg tttatgtgta ttactttttt tgtggcaaca
     6061 aacgtgctca ttatgaacat gggaatgacc gtcatgatgg ttgtgtgcca ttattcacct
     6121 atccgtagtt ttattctgcc tggaatttat aaccatttaa attagctcat aataccctgt
     6181 tatgctggtt tatatctaca acgttgtaat aaataatgca tttcttaccc tgtaagaatc
     6241 aactactttc aattataatt catcgttgac agaagaaaaa tttagcttgg caataaaaaa
     6301 ttacggagat cattgtattg tgaaaaaaat tctcatcacg ctaattggct ttttattcat
     6361 gaacacctat catcatttgc tctatgccga taattcccat catcacacgg taaaagatct
     6421 ttcaacatct tcctctaaat tggagaatac acccacaata caattggaag tccagaagat
     6481 aattgataaa aaggataaga aactggtatt tgtcaaactc actgatataa aatcgaataa
     6541 accaattact ttcaacgatc ttactgagat acatacaaaa aaatttcatt tactgatcat
     6601 tgataatcat ctcattgact atagtcatgt tcatcccgca gaaactacta ctccagggat
     6661 ttatcaattt gaatggcaac ccaatttcaa gaatgccacc tatcgggcat gggctgatct
     6721 attaccaacc aataccaata aacaggaata tgccactgct gatctaacat ctccccagac
     6781 agtaacgaga acaacgaatc ctatacatca tcgaacagta ttagagagta cagtaaatgg
     6841 atatcaattc aaattaacct tcaataaaat gcctctatta gcaggtaaac ctgcgatggg
     6901 tgaaatcgat gtgactgatt ctaaagggca tccagtccgt tcactagaac caattatggg
     6961 ggcctatgca cacattgtcg gctttaatga agattttaaa accgttatcc atgtccaccc
     7021 catgggcaaa gagcctgcaa atagcaccga acgtggaggg cctcaattac agttccatat
     7081 tgagcctaat aaagcaggtt tcattaaaat gtttgcccag gtgcaaattg atgggaaagt
     7141 actctacgcg ccatttgcat tcacagtaaa aaatgcttaa cgactaaatt aagaacctgt
     7201 cttttacaga cgcaaatatt attgtaagct cctagcttgt ggtgtgcaga gcaaaccagt
     7261 tctttgtacc cgctcattct gttttagggc agtatagtga gcggacccgt ctgcgtttca
     7321 caccaggttg atttttatcg tagagttccc agtcccattg gggtaattcg caatttttat
     7381 agataaaaag ttgattcaag tgttcaatga attgattttt attgcttatt tcgggtgata
     7441 tctgaattgt aaattgggtt aatgtctgct ggcaaattaa ataatcttca aaacttacac
     7501 cactgctaat catatgctgt cgaataacat ctgcaaaaat gggaactatt tcttgccctc
     7561 ttttcccata acaaacatca cctaaacggc cttcaatatt ctttaactca gtaaatacac
     7621 cattcgatct ttcagtcacc aaaacatcat caagacggta acgaataatt ggctgagtag
     7681 atctgtacaa gtcagtaata attggcacga atcgctcttc atccaaccat tccttttcaa
     7741 taataagaaa ctcttcattc atcaataagc gattggtttt acgttcacta atagccagaa
     7801 aaccttctgt acattgataa atttgtgata tggtacaacc aaatgctttg gttaaaatct
     7861 cttcttcctt tggttcgagt acttcggcca cagaaaatat tttttttggt gtaattttta
     7921 aaatatcttg atggctagca agcattaaaa gcacactggc tggagctgaa ataatagtcg
     7981 gttgaatggc atttaatctc tggatatgag tatcaaaatc agcaaagaga tcaaaaaaat
     8041 ggaattgtat tgttctactt ttatttaaag tgatatacaa ctggttattt gctcttagaa
     8101 aaaaagcaat tcgttctcgc gaacgcaaac cgttcggcat agccttggca agaattattc
     8161 cggcccaggc atcacgttcc tgctcactta ctaaaaacaa gcctctgctc cccgaagtcc
     8221 ctgaagataa accaacagca atattattta tcattgggga aaaatcacgt gtatgctcag
     8281 cacgtaaagc aatttccaat gcctcagttt tttttacatt tactgtatta atatcatcaa
     8341 agtgttccat catgattttt ttattaacaa ttggccattg ttccaaagga gtttttagat
     8401 aatctctata gaatgcggat tgatgcaaac aattttttat aagtttttca aattgttttg
     8461 cttgatgagt ctttaatttt tccggggtag taatagtttt attaagataa agtgtattaa
     8521 aataatgaaa tagaatacgt gctaacataa tgggttccca attctcgagc gcaattgttg
     8581 gcagtgagaa ggaataattt caatagtctt gtttctctga tacagttcgt gcaatttttg
     8641 aattgtcttt aaataggcac gcttgtcatg atgaatcaaa taggtcaaag cgctcggata
     8701 aatgagttct ttaaaagttt cctggtgcca gcaactatcc gcaaccagga aaatgtcgcc
     8761 aggcgcttta aaatacaagc cgatttgtcc tttagcgtgt cccggtaaag gaatagcaat
     8821 caatgagttg tcacccaata catcaaaacc atcagaaaag ggtaaaagat gttcgtcaag
     8881 ttggattgtt ttttgaagtg gtaataaacg ttcaaagaaa tcttcaggta gcagtccagg
     8941 taaaaaacct ttcaataacg ctttcaaccc ctttgtatgc tgaatatcgt ctatcgcatc
     9001 agaatgacat ataaaatgag cattaggaaa atcgcataat cctccaatgt gatcagcatg
     9061 aaaatgcgaa atcacaatat aattgatatc ttctggtttt atatgaattt ctagcaactg
     9121 ttcttttaag gattttacaa gtgtcatagg agttaaatga cgatatatag aggctggaaa
     9181 tttttgggtt aaacaagtaa atcggtcaga atatcctgtg tcaaataaaa tatatccgtg
     9241 gattgggtgt tttataagag tacagaaaga tggatattca cgctgtctga ttttcccatt
     9301 ttttaaagtc atgcgttcgc aatgtttgca atagccagct tcaaataact gatactctat
     9361 attcatgaac aataccattc agcaaatttc attataccct catcaatgct ataaattggc
     9421 ttatacttta aatctttttt tgcctcttca atatttaatg tttgtccgaa agccaacact
     9481 ccgacaccat actctgtaat ctttggttct gttttcagat aaagcacccg atgcaaaaac
     9541 tcaagaaact ttgcggcaaa tcttgcttga ttataaggaa tacttctggt ttttaatggt
     9601 ttgttaagag cagaaaacat ccttgaaatg atatcaataa aagtcctagg ttcatcattg
     9661 gtgatattat attttttccc tgaatagcat ttatctgcca aagcagccag gataaggctc
     9721 tccactacat tttctacaaa agtaatatca atcaaatgat tacctgaacc aatgattgga
     9781 agcacccctt ggcgctctgc tttcaataag cgagggaaaa tagcgcgatc atatggtcca
     9841 aaaattcctc tgggacgtat ggtaatcaca tccaggccac gctgtagttg agccttatct
     9901 acgatggttt cagcaattaa tttggtttgc acataataat tgaccggttt tgctggtaaa
     9961 agtgcatttt ctttaatatt gtgcttctcg gtaaaatcaa aataaatact aggagtggag
    10021 acataaacaa gtcgagcctg actgggagtt gcctgaacaa cattgtgagt ccctatgaca
    10081 ttagcctgat aaaaatcttt atattttccc caggggcttg ataaagccgc gcaatgaaat
    10141 atgaaatccg cattttgact tatcaaagac aaagactcca gatcctttaa atcgagcgga
    10201 acaaatgttg ctcccatttg gcttattatt tcacccaact gcaaattgcg tcctaatgca
    10261 atgacagcat ggccttcctt aatcaagcgt cttgtaagat ttaaacctaa acatcctgta
    10321 gcgccggtta caactgaaac catactaata ctccagtatc attccagcag ctgacaatcc
    10381 agcgccagta cctaataaat aaaccaactg cccacgctct aaattatgat tatggatcaa
    10441 atgacacaat gcagaaggca gagaagcagc catttgattg ccatgcgtcg tgtaaatatc
    10501 aacaaatttt tcaccaggga tgccaagctt tttacgtata tgatgcatag caagcaagct
    10561 ggcttgatgt gggacaaccc aatcaacatc atttagagtt atactagctt tagcaaataa
    10621 ctcctcttgc atgggttcaa tcatttggct ggccagtttg aatacacgct ttccatccat
    10681 tttaaataaa ccgtatttct tatcgtactc actcgcaggc ggcatgagtg ttcctccagc
    10741 ctggacctcg caaaaggcag cacccaaact gtatgttttc atatgagcag aaagaatccg
    10801 actggaatca ttgcttcttt caacaacaca agcagctgcc ccgtcaccaa aaatcgtaca
    10861 ggtttccaag tcgctccaat ccaggccaaa tgaagggata tcacttgata caataagtat
    10921 acgattaaat ctgccccctg agagcatata tgatgctgca tcaagtgcag tgagaaaact
    10981 aagacaggta ctgttaatat caaaacaggc gattcctgaa ttatctaatc ccaattgttt
    11041 ttgaaccaga accgccgtac atggtatagg ttgctctccg gctccacaag ccccaataat
    11101 ggcatcaatt tcagctaaag taattcctgc ttgttccaca gcgtgtaatg cagcttgact
    11161 tgccatatag gaggttgttt cattgactct ggcaaaatgc ctggaaacta atccggactt
    11221 cttctgaaca ctgccttcag gaagattgag ttttttatcc agatcagatg aaaacactat
    11281 gtttgcgggt aaatattttc ccattccgat gatttttgct gacgacatag aactgacttg
    11341 ttaaagggtg gagcaattat tgtactataa ggcttattat agtcaaagag aactttttaa
    11401 aagttgattg gtaaataatt gccgtgtatt tgtataaatg ataatctatc taatcttata
    11461 tattatctaa aatatgcatg aaaatcctga ctgtactgga cagctatcct cccgacttaa
    11521 atggtggtgc ttattttact caccgtttgg caaaattctt acaagccaaa gggcatgaag
    11581 ttcttgtgat atgcccctct cgaagcctga agcaaggtta tacaagctat gaaggagtca
    11641 atctctatgg agtacgatca tggccagccc ttggttacaa aaactttcgt gtttgctggc
    11701 ctttcttcat caagaagggt attttaaaag caataacaga ctttaatccc gatgttgttc
    11761 atttgcaggg aaaatttttc ttaggtggaa tttgctatcg agcctgtcgt aaaaaggata
    11821 tcccgctaat agccactaat catttcatgc cagaaaactt tttccattat actcatcttc
    11881 ccaggtattt tgaaaaatgg tttcatcgca ccacatggaa tattgtgatt gacatgctaa
    11941 accatgtaaa gatagtcact actcctaccc acactgcagc taacttactt aaagaagtgc
    12001 acgtacaaaa agaaattcat gttgtttctt gtggagtgga tctacaaaaa tttcaaccaa
    12061 aacaaaatgc caatctcatt agacaacgtt ataaaatccc tgataagcca attctattgt
    12121 atgctggtcg tttggataaa gaaaaaaatt tatcaatagc cataaaagca ttttataaaa
    12181 caagacaatc cattgatgct cattttgttt taaccggttg tggcgcagaa ttacaaagat
    12241 taaaaaaact ggttcagaca ctgaatctca ctgagcatgt cacttttaca ggatatctat
    12301 cagacgcgga atatccgctg gtatatagtt tagcaaattg ttttgtaaac ccgggaactg
    12361 ctgaattaca aagtattgtt gccttggaag caattgcttc tggattgcct cttatagctg
    12421 caaaagcgat ggctttgcct gaacttgtta aagaaggagt aaatggttat ctctttgacc
    12481 ctaatgacgt tgagacattg tcttgttata tggtcaagat tcttagtgat cggactctca
    12541 gtgaacaaat gggaagagag agcagaaaat tatctcaaga gcatgatatc aaacgaacta
    12601 tcgaacaata tgagacactc tatcaatcga tggtgagttt atgaaaacag tcgcaaatcg
    12661 ctaccatgtt ctttgtcagg aaaaagtatt cataagaagt ctgcttagtg gctttttttt
    12721 gctttttata agttttctca ttatgaattt tgcaggaaaa tatgcttcac ttatggccgg
    12781 taatccggta aatgatttaa ttcttgataa tttacctctc cgtgatgtaa ctgtttttca
    12841 tgttaatgca gctattatct tttggacaat ttttactatt tataccctgt taaatccagc
    12901 gctcattcct tttatcacta aaagtgcggc gctgtttata ttgatacgtt gtgcctttat
    12961 tagtatgacc catctcggtg cacctcctaa tcaactgtct attccagata atttctcatc
    13021 tcttgtttta tttgatggag atttattttt ttctggacat gtcggaggcc cttttttaat
    13081 gatgcttatc ttttggaata agacaacatt acgttatatt tgccttgctg ctgctgtttt
    13141 tttcgcttat acggtgctga ttggtcatat tcattatagt attgatgtct ttgcagcccc
    13201 atttattact tatggaatat ttcatataag ccaattcctg tttgcatcag attatcaacg
    13261 actaaaatca gaacttacat catcaactgg aaattgataa cagttgcgtc atactgtagg
    13321 attagcccgc atgaggaagt aattaagtaa atatatggct gagataatca attccagctt
    13381 atccctattc aaataatccc agtaatgttt caacagggtg agaagctttt aaaccagtta
    13441 atcgtttgac ttgtgctcga catgaatagc catcgactaa agtattagat aaacttcaaa
    13501 ataattcttc catgataaat caaacaagcc atgggaatta agttgatgga ttgactcatg
    13561 cccataagct ccagccatac cacaacaccc tgttttttca gcaactagag tcaatccaaa
    13621 aaattgaaag actttctgcc actgtattaa agcttcactg caatttgcct gttctgtaca
    13681 atgacttagc aataaatact gtttaagctt ttcattagct tttattggta actcgttgat
    13741 gttttcatta cccagtattt gcgagagcca ctcttgaatc aacaggattt tactattgta
    13801 attttcgtgt aaatattccg aatattcata tcgataagtt aataccatgg ctggatcaag
    13861 tcctatcatg gtaaatccat gatctgatag cgttttaagc attaaagcat tttttctggt
    13921 aatttttgca aatgcctgaa gcattccctt gtcgtgcaaa gttttaccat tttcaaacca
    13981 aggtaacaca taaacctgga accccagttt ttttattaac tgacaagctt tcagcacaag
    14041 ttcagcttca taaaaactgg tcacccaatc ctgtatataa attacagttt tctcactgac
    14101 aggtaaatgg tttaaatcaa gccatggaat attatttgcc tttaagagtg acttcagatt
    14161 tggactacta aacaaaggga gatcaaccaa ccctgtgtat ttatgtaaac aatatttcac
    14221 taatttattt gagagacaga aattagttaa ttctggaaac aggctttgaa aatatccaac
    14281 acgttcgctg taagcaataa gataatcttt tattgagcga gaatatcgtg tgtgataggt
    14341 ttgcaaaaat tttgctttaa acgaaggaac attgatatta accgaacatt gagagcgaca
    14401 tgcctggcat cccaggcatc cttctagcgc agtataaact tcatgggaaa aatcatattg
    14461 gccgtgattc cttttgtaag tatttaatcg ctttttcaac ggccatgtta attcacgctt
    14521 tcctgccaaa gcacttaatt gccttaacca ttccctaacc aagctggctc ttcctttagg
    14581 agaatgaatt ctatccctgc ttattttata agaagggcac atcacatctt gtaatttata
    14641 atcgaaacac gctccatttc cattgcagtt taaaggtaat tgataagaat ctctgtactg
    14701 gtagttaatt tgattgtcta catctgctct ggttttttcc gaacggatgc tggcaagtga
    14761 gtcatggcta cctatgggga cagcaatttt tccaggattt aattggttga aaggatcaca
    14821 gcttgattta attttcctga gctcattgta gagttcctct ccaaaaaata caggcacgaa
    14881 ttcacttcga aatcctttac cgtgctcgcc ccacaatacc ccaccgtatt tatgggcaag
    14941 ttgcgccacc ttttctatta tttcgtaata gatttcccta tcttttgcat ccatcaagtt
    15001 caattcgggg cgaacatgta aacaccctac atcaacatga ccaaacatcc cataactaac
    15061 cccatgagaa tcgagtaact cacgaaattc cttgacgtag tcagccaaat actctggagg
    15121 aacaacagta tcttcaaccc ccgatatggg ctttcttacg ccctttactt tacctaaaag
    15181 tccaacactg cgctttctta gctcccaaag attgataata tcctcattat tagtaacaaa
    15241 atgaaaatcc gtacctttca aatttttctt caatagttga gcatggtgta gtaactcttt
    15301 ttcatcgttt gccacaaatt caatcaaatt aattgcatca acttggctgt tgcttccaga
    15361 attcagaaaa ggtttcagcg aatcataaat aatatctgtc ttggccaatt tcaaaatagt
    15421 gctatccatt gtttcaatgg cttctggatc aaatgccagt aaatgctttg catgttcaag
    15481 ggcatcgcta aatgaaggat acaaaacaac aaacagacat ttgtatttgg gtatactcgt
    15541 taattttaat ttgattttag tgacataggc caaagtacct tcagaaccag cgagaatata
    15601 attggcattg aatctcttgt catcatttga ataaattttt gccaaattgt aaccagtgac
    15661 aaaccgatgt aatttaggta attgcctatc aattgtctct cgatgttcct ggacagtttt
    15721 atccaattgc ttataaaact gccctacctg cccaggcatt tttttcatat tatcaagaag
    15781 actgacatct atctcttcac tggtatggac ttctcctcca agatagacag ctgtcaatgc
    15841 aagtatgtgt tgactggttt ttccatacaa tcttgagcct ttgccactgg catctgtatt
    15901 aaccatacct ccaatggtag cgcgattact tggggataaa gcaggtgcaa agaatttgtt
    15961 ataaggcttc aggtattcat ttaattgatc aagaactact ccaggctcaa ccaaaaccca
    16021 gtcttcatac gggtttatct ccacaatatg attcatgaaa cgagaacaat cgataattat
    16081 tccagcattt aatgattggc cattagtgcc agtgccgcca cccctggggg tgaaagttaa
    16141 atcacgaaat tgctcgctac tggctaattt aaaaatatga gatatatcac tttcactttt
    16201 agggaaaata atggtttcag gaataatttg ataaacacta ttatccgttg ataaaacaat
    16261 tcggctactt aaagcttcat caatctcacc atgaaaatcg ggtattttct caagttgctg
    16321 taaaaataac ctgatttttc tgggtgttgc agtggaagag gtcaaacgcg ggatcatagt
    16381 tttttcctag cttttcctcc attaatcttc tcattataga ctaccagata attagggcta
    16441 aatcataaag acgactacca ttcaatcaga aaaaatagtc tttttagtct aaattaagga
    16501 tacaggcatc tttttaaagg attaaaacga tatgaacaca ataacagaag attctctgac
    16561 tgaagaacaa aaatacatgg ttcaagtatg ggaaaatcat acccattatg aatttgacac
    16621 taaaaatgtt gcagatacta tgaaaaccat ggaagacaat ccctatgtca ataacatacc
    16681 taccatagca ggcggatttg gacaagaaga agtcaaacaa ttttacagcc aattgtttat
    16741 accccaaaaa ccggatgata ctgaggttga actgatttca agaacagtag gtactcatca
    16801 aattgtagat gaactcattt ttaaatttac tcataatatc aggatggatt ggatattaaa
    16861 tggcatcgag cctactggaa aacgtgtaga aattcctcta attgcgattg tcagatttca
    16921 taatagaaaa atcataagcg aacatattta ttgggatcag gcatctgttc ttgtacaact
    16981 cggtttactc aattcagata aactgcctgt tcatggagta gaagccacca ataagataag
    17041 ggaactgata tcacgaagtc tgactaagta agaaaaaacg cttacttata actataatta
    17101 tttttaaaga atacagggat cgtcatgata aaagattatc ttatagaaac catgaatcag
    17161 gatgaagtga atcttgctat aagctgggct acccgggaag gatggaatcc aggaattcat
    17221 gatgcgacat gcttttatca aactgatccc catggttttt ttgcaggtaa gctgaatgga
    17281 aaaacgatag ccattggttc tgctgttatc tatgatgaat tttttgcttt ttgcggtttc
    17341 tatattgttg ataaacccta tagggaacaa ggttttggtt tggaattaac taaagccaga
    17401 ttagcatata ttggtaacag aaacgcggga attgatggtg tgatcaacat gctggataaa
    17461 tatgcccgca ttggttatca attagcacat aataacgcca gatattgtct taataagaca
    17521 ataaaaccaa tatcatctga acctcatatt gtaccattaa atcaaatcga tttttcgcag
    17581 ttatcatcct atgaccgctt acattttcca gcacctcgtc ctcaattttt aaaatgctgg
    17641 ataagtcagc cacaaagctt ggctcttgct tatttaattg atggccaaat taagggttat
    17701 ggtgttatca gaacatgtca taaaggattt aagattggac cactatttgc ggatactccg
    17761 gaaattgcac accaactttt cactaatttg gtcccatatg ctaagggtca attgatattt
    17821 ctggatattc cagagaataa tcctttagca attgagctgg tcagtcaaca tcaaatggag
    17881 aaggtattcg ctacagcacg tatgtattta aaaggagaac cccaggttaa aaaagaaagt
    17941 atttatggaa ttactacttt tgaattagga taaaccatga cccgagatct cactggatat
    18001 ggatcagaac aaattaattt gacctggccc aataaggcta aaatcgctat taattttgtt
    18061 attaattacg aagaaggagc agagttaact ccacttaacg gtgatacaaa ggctgaattt
    18121 tacggaagtg attttccttt cgttcctaaa ggaaatgcag aaaggaatta tggtatcgaa
    18181 tctatttatg aatacggaag tcgagtcggg atttggcgtt taatccgtct ttttgacaag
    18241 cattccatcc ccttgacgtt tttcgtaaca ggttatgcct taatattaaa tcctgaattc
    18301 tcaagctatc ttgcaagtca taaccatgaa gttgctggac atggatggcg atggattgat
    18361 tattcaaaag aaagtaaggc aacggaaaaa aaacacatct ctcgctgtat tgataccttg
    18421 caggaactca ctggacaaag gccattgggc tggtacacgg gaagaagaag cccatttacc
    18481 cgcgatctgc taactcaaat tggtgggttt gtttatgatt cagatagtta cgccgatgaa
    18541 ctcccctatt tcattaacaa gcatcttgta atcccatact cattagactg taatgatttt
    18601 cgtttcacaa tttctcccgg ctttgtcacg gcacaggatt tctatcaacg tcttaaaaat
    18661 acttttgatt acctttatca agaaaatcga ttagctatca tgactattgg tttacatccg
    18721 cgattaagcg gcaagcccga tcgttgccat gcccttaatg aatttctaag ctatattcga
    18781 caatttcaaa atatatggat agcaaagaga atagatattg cactgtactg gttaaccaaa
    18841 cacacccaga ttaagcagga tttatgaaat acccaatctc agtgttagga aatgttttta
    18901 attcagtcgt ttagttcatc taagattctt cgttaaaaca ttttttgccc tgatcgtgag
    18961 gtttttcgta gtgctcttat ggtttgatct tattaatttc tgtactgttt catcaaaaga
    19021 gcttaaaatt catatctcaa aatctttata attcacccca ctataattta cagattgcca
    19081 atatgagtca tttgctagat ttatccatcc ttaaaaaaaa tcgcgacttt accttgcttt
    19141 atttgggcca attcatttca ttcataggca caatgattac cagtgtcgcc cttccctatc
    19201 aaatttatca tttgaccgaa tcgaccatga tggtaggatt actcagcttg gcacaattac
    19261 tgcctctact gattactgca ttgcttggtg gagtgtttgc tgatcgatat agccgacgcg
    19321 gattactaat cattagtgaa ttattacttg ctgttggttg tctttttctg gctttcaatt
    19381 ccaaactggt taatccaagt gttagcttga tatttataat agcctcatta atgtccgcca
    19441 ttacaggttt acataggcct gcgtttgata gcgtgataca acaaatcgtg aaaccagagg
    19501 attacaagaa agtaggcgct cttggcagct tcaaattcag cttttgtatg attattggac
    19561 cagctgctgc tggcctgatt attgcgcaat atggtattgt aatcacttat ctcattgatt
    19621 tattaacctt tatcatttca cttctcaatt tgagcttaat gcacaccata cccaagcctt
    19681 tagccaaaga gcatccctcc attatagttt ctttgaaaga gggtataggg tttgctttgc
    19741 gccgacaaga gctgatgggt agttatttcg ttgatttctt tgcgatggtt tttgctatgc
    19801 caaatgcatt gttgcccgct attgcccaac aatttggagg agctaaaaca ctaggactgc
    19861 tttatgctgc tccagctata gggtcgctga ttatttcttt tgttagtggc tggactgcaa
    19921 aaattactca tgatggaaaa gcgatcgcaa ttgctgctgc tttgtgggga gtggcaatta
    19981 tcggatttgg tctcaccact tctttgtggt tatctctctt gtttcttgct cttgcaggag
    20041 catttgatgc cattagtggt attttcagaa gcagcttatg gaatcatacc atcccgcaag
    20101 aattgagagg ccgcctatct ggaattgaaa tgattagtta tttgagcggc ccaagattgg
    20161 gtgataccag agcaggcttt gttgcagcag gtttaggcat acctgctgca ttaatatctg
    20221 gcggtatttt atgtattata ggagtgactg tatgttgcta tgccttgcct aaattttgga
    20281 attatcattc aaagtaatta accactttac taatctccaa taatagaggc ctttttatgt
    20341 tgttgcttgg tagtctcatc caaactggcc gcttccgtta atgcggctac attttccttg
    20401 gtaaaaaatc catcaaaatt gtgacatctt tcaacatatt cagtaattaa tatggaataa
    20461 atggagtgtt tggagaaaat ttgctttaaa tcaggcgaat cctcgcaagc cccaaatact
    20521 ttagctaaaa attcaatatt taatttatct cgaagagttt caacgacaaa atcaatattc
    20581 tttttaccag aagagtgatc cccgtctttt acttcataag caatatgatg cattcttctt
    20641 ccgtagttac gcacaaaggt ttctgtgggc atagggagat tttcaaaaga attcaccatg
    20701 aacggagtgt tgttagcagt aaacactttt gccggagact tgatgtcatg tccgtgtgga
    20761 tttctattta cattggtaga ggagttcata tcatagatat tataagcacc ccaaaaataa
    20821 tatttggata aacataaaaa ctctaaaata gcatcctcac gctcaccagc caggattcga
    20881 gtagccatat ggtcaatgcc tttgatcaag tccttgatcc cattatcgtg gctttcctga
    20941 tctttcgcat ccagcatcgc ctcttgttct ttggttaaat aaaatcgttg tcccaattcc
    21001 agacagtcaa aatcataaat attatcatcg gtatacccag tgcaattgta ggtaaaatca
    21061 gataaccggg taaaatgtat atgtttatta caatagaact tgttttcagt catatcagga
    21121 ttatgaaaat taaaatcatg agaaactaat atgttacgtg tctcatcctt atccttgcat
    21181 ttataaatct cacctacata acgagcattg aatttggctc ttgagttggg atacatcttg
    21241 tttaatcttg tgatatcgtc tttaaaatta ggatccagtg gctcaagcac cagaaaagca
    21301 ggagaatttt tggtattgag taaacaataa attttatgcg tgtcactaat ataacttcga
    21361 gaatatcgat aaggggtcat tgagtagagt tctttaatat atggaatagc atctcccgtt
    21421 tgtacctgaa caactacccc acacatatca ccaaatagat cctcaagtcc ggatgataaa
    21481 cgcctttcgt aaatcttggt tttatattca ttaaaatagt ctgaattgac tttatcccct
    21541 ttgggatggt attcaattgg attaatcatc atgttctccc caatttgatt caccggcaca
    21601 taatatttta agtatatacc tgccaatgaa tttttatttg aattaataac tgtttcctct
    21661 ctttcatggc aaaatagagg tataataatt cattttgatt ccttacatac tttccaatgg
    21721 aagctttaat cgtatcactt ggggtgatta cccttgcaga aattggcgat aaaacacaat
    21781 tactcgcctt ccttctggca gcacaattta aaaagccatt gccaattatt ttgggaatat
    21841 tgactgctac tttgataaat cattctttgg ctggactcat cgggatctgg attaccactt
    21901 tattaaagcc gaatgttttg cgttggatac ttggcatatc gtttattctg atggcaatat
    21961 ggacgctgat acccgatgaa attgaacagg atgagaaaaa aatatccaaa tatcttggtg
    22021 tttttggagc aacatttatc actttctttc tgtcagaaac aggagataaa acacaaattg
    22081 caaccattgc cctggcagca cactatagct ccccgatcct ggttattgcc ggttcaaccc
    22141 ttggaatgtt gcttgctgat ttaccagctg tttattttgg aaatttattt tcccataaaa
    22201 ttcccatgaa aataattcat gctgtcgctg catgtgcttt tctaattatt ggactcatta
    22261 ccatcttgtt ttgaccgact aggcaggatt gttatttaac ataagtatct agccagttgg
    22321 tatatctttt taatctgtcg atgacaaagc ttggtttctc caaagtatga tattcattgg
    22381 ggtaaataac cagctgtgtg ggcacatcga gtgatttcaa agcttggtat aattgttctg
    22441 acccaacaca aggaacatta aaatccaagc cactgcacaa aaataatgta ggtgttttta
    22501 tattattcgc tttaagaaaa gggtagctta attttagata caattcagga ttggtccagg
    22561 gctttcctaa ttccagctcg tattccggcg tgtattgatc gacaccataa ccagctaaaa
    22621 tattcgctgc tccagcacct gatatagccg ccttgaaacg actatctgta gctatgacat
    22681 aattggttag cattcctccg taactccatc cgcctattcc caaacgattc ggatcaacta
    22741 ttcccttccc tatgacataa tcaacagaag ccaatatgtc ttttacatcc aaattacccc
    22801 aatctgcaaa aattgctttt gcataatgaa acccccttcc ggaactccct cttggattag
    22861 gagcaataac ggcataatta ttggctgcta tccactgcat atcaaagtta aattcatggc
    22921 taaattgata gactggtcct ccatgcagat agacaaaacc aggaaataaa atacccggct
    22981 ggtattccgc tggcttcatt aataatccat caactcttgt tccatcaaag ctattgcact
    23041 gtatgtcttc tgcgggttga aatttaactt catccaatag cttttgatta tgttgtgtta
    23101 aaggaattaa tccggaattc aaaataaata attcagaggg atgtgtatca tctgttgtta
    23161 acaaaataat tttccctttg gcaagtgaat aatccatgtc atagcgcaag ccgcctgtca
    23221 atttttttat ctggccggaa tgaatgtcta tgcgattcaa ataggtgttt ctgctttctt
    23281 ctattaatgc ataaataaat ttgccatctt gagaccattt tggcttgtaa taccaattat
    23341 cccgaggtgc aatgattttc tcatctttag tattgatatc aataattgcc aattgattgg
    23401 gcgaataata ggtccattga tgttcattac tgcgaagata agctattttt gaactatccg
    23461 ggctccagga aagatttgaa tcgtaatcag gattcatatc actaccgggg aaatgagtga
    23521 gttggtgctc ttttgcccca gacttgggtt cgatcaaata aatatcataa ttaaaatgcc
    23581 tatccggatc ttttccacgc tttgtgacaa acgcgatgta ttttccatca ggtgaccagg
    23641 ccggtaaata ttcatcatga tttccatcag tcagttgagt aatagcctta ctcttaagtt
    23701 caaccaaata taaatgatct cttcccttac caagataacc gacaccatca accttaaatt
    23761 gatatctatc tacaactatg ggttgttctt cctcattaga tggatcactc ttctgagcta
    23821 taaatgcaat cctgttagaa tctggggacc atgtaaaatc gctcacttcg ccatcaatat
    23881 gagttaattg atgttcttta ttatcctgga cagatgctat ccaaatttgt gtcgtaccct
    23941 cttttccgcc atcggataaa taagctaacc atttaccatt aggactccat ttgggtagcg
    24001 agcttgatga atcttctgca aaggtaattc gttgactgga tttactatcc actgatactt
    24061 tccatacctc agtcgtggaa atgttatctt tggcatttaa ttgggatacg gtataggcaa
    24121 cccattttcc atctggtgac agatctggct cgctgatatc ttgtatacga taaagatcat
    24181 taagttctgg caaacgcttc ccagcccatc catcatgaaa tacaacaaga caaataatta
    24241 gacccaggca taaccccttc atgacaatca tccttattat gctgaaaaat cagagattta
    24301 tagtgcctta aaaagtatag aataaatttg ttatatatga tttttcctgc aatcagcaaa
    24361 caaattttga gtggaataag tacagtctgg tgtcattgat taagaatcgt agctaaaatt
    24421 cttcagaaat aaagaaatcc ccgtctgttc aacgaggctc ataaaaaggt tctaatttat
    24481 ttctggtata ctttttacgg catgatccgc ttctgttctt tgaacaaact ctctaaagaa
    24541 agtctgcgcg ctcttacctg atcgattatg caattcattc cagtctgaat tatgaccatt
    24601 tgcatattgt acatcagtct ccatttttct gtcacttttg attttttccc tagcttgaac
    24661 taaagcgtct cttgcattag ccaaccctgg tttcgcaaga ggatcagctt ttcgaatgat
    24721 accatccaga tttgcaattt cttcatcaat ctccgccttt ttcttatgcc gctcagaagt
    24781 caattcttta tggatagatg cataatgtgg aatcacagca tcatcgatac gctcacccct
    24841 tatctctttt cttgacctaa ccactatgta atctttatat gcttcaggaa cactcttgaa
    24901 tgcactgcct gcattaattt cccatttagc taaagcaaca ccgaatttaa tgaagggctt
    24961 agccaaccat ccaagtattg tccctactgt gctgtcttta tgacctattg ctcgaccaat
    25021 ttcatcacgc tctaaacgca tatgccctac taagaaatta gtaatagtag agaaagaacg
    25081 gctattaaat agattaattg gctgtccaag ttaatgaaaa tgttgcgcta ccaaagaagc
    25141 cacgcctgca ccaagagaat gacctttcag ggtaatattt tgtggcgaaa caccttgatc
    25201 taatagtctt tgcacttgtg caataccatc tgcaaccaaa tcctcacttg atttggcttt
    25261 gcctgtactt tgaccaacac cacgaagatt aaaaccaata acattggcct taagagcctt
    25321 cgaatcttct tttatatcat caatgatatg ttcataacac ataccattcc ctaccaaatt
    25381 gataatatat ttttggtatt tgggatcgat actttcttgt ggcctatgcc ttacttcaaa
    25441 tgtatctaaa tgcgctccat catgagtaat tacatcatgt ttctcacaaa tcagatcgtc
    25501 ttcattataa ttacttacag tatcatcact aacagctaaa tcatcaaaat tttcattttg
    25561 tgctggcagc actaaaccac taacccattc accaagcagt ttatttgccc caatttttat
    25621 taaatcccat aataaaacag ggggaaaaac cgttcgaatt gcaataaatt taaaccattc
    25681 ccaaccacta tatttaggtt ttggtgcatt ataaatttta gccatgagtt taatcctgtt
    25741 tacataaaat tgtacatatt atatacagta aatattaatt taaccttaag ctgaatgtta
    25801 taaacttaaa aaaatagcct aatctgggaa ataaaaataa gtaaaggatg cgtgatcgct
    25861 caatattctc aggacttacg aaaaactcca aggtcaaggc aaaaaatgtt tttaatgagg
    25921 gagtttagat aaactaaatg accgaattaa aaacattttt taacatagag attggggttt
    25981 ttcgtaagtc ctgattctat cattatgctg acttagctta tgagtttgag ccaatcaaag
    26041 acaatggtga taattaaccg atgctattat atgccatcac aataatttgc ttctgtaatg
    26101 gccttcattc aactaagaaa tgatacaaag aatgatagtt tggctctatg attgaataat
    26161 gttgttttct gcctaaaaag accttcagct gttccgggac aggaatttct ttgttcaata
    26221 gtggttctaa tacctcttca aatttggccg ggtgccctgg tgccacaata acccaggatt
    26281 tgtcctgatt gattgtactt agacgatgat aagctgtggc ggcatgaggg caaattatat
    26341 aatgatattt attgtaacaa tgtaggatag cttgcttaat ttgctcatca ctaacagatt
    26401 ctgcattcat ctgttttcta cattcaggat aattattaaa taaaatgaat aaacgctcca
    26461 aattgcttgg ctcacctaca tccatcgctg ttgccaatgt tttagtaacc gatttggctt
    26521 gataagctcc cgttaacaaa aattcgccca gtgcactatt agtattatta gctattaaaa
    26581 tttgctcaat cggaaaaccc agcatttttg cccaataaca agccgtaaca ttacccaggt
    26641 ttccgcctgg aactataaaa ttaacagaat gattatgtct taatgccagt tgaatgctgg
    26701 taaaagcata gaaaataatc tgtggtaata atctggctat attcatgcta ttggctgtag
    26761 tcaatactcc tttatgttct cttttggcaa ataccagctt caccagctgt tgacattgat
    26821 caaagctgcc attaacagcc aatgcacgaa tattctctcc ccaacaagta atttgagatt
    26881 gttgtctgaa agtaagttta tctttgggaa ataaaatgat tccttccaga tctcttttcc
    26941 catgcaaagc atgagcaatc gcagcgccag tatcaccaga tgttgcgacc aggactttgg
    27001 cgctcttgtt ttctatcaga tgcaataaac attcaatgaa aaattgagtt cctaaatctt
    27061 tgaaagacag ggttgggcca tgaaacaact ccagtacata acttttctta aaaagatgat
    27121 gtagcggaaa ggagaaggtg aggaattttt gaaaaaaagc attatttaca tcaattttac
    27181 tatctgaaaa aaatggcgag agtaaatgag cagaaaagtc gattaaatta ccgtattcat
    27241 aaaatttttt tattgttaaa ttaggaaacc tgtcaggtac aaataaccca ccatcccctg
    27301 ccaggcctga cagtattgct tcgtctattg tggagacaca ttgtcctctc gtactcttta
    27361 ttctaatttt catctgcagc ctttgcacct tctgatgaaa tattggtaac caataaatca
    27421 ctttcaaaga caacttttgc ttcgcggtat attgctaaca gctatatttt agtcaaccta
    27481 atttagaaga taatggaagt ttagcttatt ttttacaaat ttattaagat tgacgtgaag
    27541 taacatcagg caaaacgcca atttaaacct aatttgaatt gttctaaaat tggatgtttg
    27601 gcgattctgg tcgtttgata cctatgatta ttagaatccc taccctgtga atcaaagata
    27661 aaatcctgca accgattcca atcccactct acacggcaac gccaattttt ggttaactcc
    27721 aactctaggc ctgtacctaa ttggactcca ttcttttttt tggtatttga aaatcttcca
    27781 ctgaatgagc caaaagggcc actctgagta atgcttccgc ttaggttgac atcactgaca
    27841 atccagccta cccgtccata gatgattccc acagaaggca aataaatacc agggtgtata
    27901 gctagaccag actgccattt ttgtttaaat gaacccttta atattcttga gtaaagttcg
    27961 ttagaatcaa aaaaatagat ttttccttca ttcgaactgc ggttaaaaaa accttctaaa
    28021 ccaatataat aatgtgtccg gaaaaatttt tcatagccta aataaatttc tcctccaagt
    28081 cccgatccat gccagtcaaa atgatcaaag tcagtaaaat tgagctcttg gctggtttca
    28141 tcccttttaa aattgtaatg ggctgtgtct ctaataactg aaaatccaaa atacgtatcg
    28201 ggccttagaa aaaaatttag cggtgttttc tcagccgcta aaacaaataa aggtgtaaaa
    28261 ctgaaaaata aaaacaacaa gattgattga acaggaaaac ctaaatacac gaataattcc
    28321 ttttaataag tcaaaaaata gccattatct tctattgcgc gtcactattt tgcatcaatg
    28381 acaaaagcat caattgtact tatctcatga ccaaaactct atatttttaa gaggaaaatc
    28441 tgcatacaac tatttaatag tttgttactg tctatttaac agctcatatc actagaatga
    28501 aagacttcat aataatcaat aaaatcgctg tcttatatgc gttctgaccc tgattaaatg
    28561 gcatggaagg atttttgaaa gctatattga tatgaaacta tactgtttga ttactctggt
    28621 ttaaaacctg gtctgtcctc tccaattttt gtcactttat tctcgctatc tacatcgtag
    28681 tcagacttaa tggttctttt tgctggattt gtattattac ttgtttgagg taatacttga
    28741 ctgggctggg tttctgtttg tgttatttct gcgttatctt ttggataagc atttaaagcg
    28801 ttttcatctg tttttttaat tttctgtaat cggctcctca attcttccaa actagacaac
    28861 tcaagaggat cttctgatgc accatgttgt ttaatacgtt cgcttattag aaattccact
    28921 gtgtccaaac aatcattcac agtagtgaga tgcatttcta atttgggcaa atcttcgcaa
    28981 tgctcacttt caagtgcctc gatttgtttg gttatttcaa ctaaaagtgg actataaccc
    29041 gactctactt tttcagcatc atcgattaca ggtgttgaaa tagggctttg ttgtgctatt
    29101 acattcagac tctcaatcgc atcttgcttc atctctataa tttgtccttt taaaccagaa
    29161 ctgattgagg ttgttttgct catatctttg tctggtggga atgcactaag tgcttgcaaa
    29221 gcagcaggta tgtctttttt atcaatagca tctttacatt gctccaaaac tggcaatatt
    29281 ttacctcgga taaatactct ctcttcaata agtcgcttta tcgcatccat ctgcgcttta
    29341 gctacttcag actcttgtaa gttaaatgct gaccattttt cgttattagg aaactctgct
    29401 aatacctgtt ccaattgctt aagatcattc ctcaccaggg cagcaaagca tttttccata
    29461 gctggattca cacttttatt gagaaaattt tggtatgccg ttttttcagc actagcctga
    29521 gatgagcggg aaattatact gataaaacta ctatcagata attgcgcttg ctttttcatt
    29581 tccatttgaa aacggaatac cattgccaat tcattttcct gatcagcaaa tgcctctttc
    29641 tcaacagcat tttttactgt tactactttc tcactctgtt gaataaaagc tgtttctaac
    29701 ttcaaatttt gtaacgccaa atcaattgtt aattgagaac gttcttgctg gctagccaaa
    29761 acaggttctc cacctgattt aaaactcctc atatgctcca tgaaatgttt ggcatcttcc
    29821 tgagctgctg gctcagatag gtaatttcta aatgattcat tttttacagc gcttaatctt
    29881 aagacatcac gacgactttt aatcaaatca atatagcctt gtttgtcggg cttatgccca
    29941 tcgggtatgt atgcggagac aaagcgttca atatattcat ctggtataag gcaaattttt
    30001 aacatcgcct gattgacttc agatctgaac gcttcattat ttgtaatagt tggttgaagt
    30061 attctttgta aagaaaaata acccccttta taatccaacc aattattagt tgcaaaatca
    30121 accggccgag gtaatgaatc aatcacttta ggagtgagat caaatctctg atctacgcct
    30181 aacatggata cgatgcactg atcaccatct atcttaatta ctctaccatc tttatctaat
    30241 cctatattcc cattttttaa gtcaacttct tgcaaaaata ctgatacaaa aactgcttgt
    30301 cctaaaccag taaatgttcc gtcctcaaaa ctcttaccct gaccatatgg taggtttttg
    30361 aaaccttcta cttcttcaga ataaacatag taattatttt gtgcctcatc ttttgcaata
    30421 agcgtttctg gctggtgagg gatgattaat ctaaaaaact cttgtgcgag tacctcgttc
    30481 cttgctgatg caggatcgct acacactttg gcaaaccagg tcttgggatt tccaggaaat
    30541 gttcctcgtg ataccatatg gttatggtgt gtagttgacc taaaaacaga attatcattc
    30601 tgaaaatgtt taaaattcag taaaggtata ggcattctta gctccaaaca cacttttgat
    30661 ttaataatta actattagta tagtaattaa tctaaatggt gtcagataat gcctgtcatg
    30721 tgaaataatt aaacttattt cataggtcta ctcatttatg gattacatgg aagtctggca
    30781 actatttttt ccgtgtcgca gttattttaa agcgtacgat cacttcatct tgtatttcat
    30841 cagtactcgc ccattcacct tgtcctaccc cgaatgtact gcgtttgatt gttgtgctac
    30901 cttcaactaa agcctgatcg ttagaaattt gatccacaac aaacttcaga gtaactggag
    30961 ctgatttatc tcttatagtt agaatccctt ctgcttcata ggttttatca tccaatttat
    31021 taaatttcga agctttaaat tctgcattag gaaatgtctt gacgttgaac cagtcagggg
    31081 aagccaatgt cgttacgatg tcctcaaaag gtgctgcaat cgaattcata tcaatagtga
    31141 tatcaattga ccctgtcttg tagtttgaag ggtcagcaaa aatttgacca gtaaatttct
    31201 taaatcgccc tgtaaccggg gcgccatttt gagtaccagt aaaagtaatc tcactttgtg
    31261 ctggcacgat ttcccattca gggagtttac tggcataagt caatgtattt acaaaaaata
    31321 tcaataccca aagggaagat ttaatataga cattcactat aacattctcc gtaatatatc
    31381 atccttatta ataaaatgat gttttaatgc tgccgctgca tgcaggatta tggccataat
    31441 taatgcatac cccaaccatt catgaatcaa ttggaacaaa attcttctat tctcatcggg
    31501 tgcaacaagg tcaggtaaaa caaataaacc aaaaaaagaa gcaggcaatc ctgcagccga
    31561 ggtaattagc catccactga agggcatagc aaacatgaaa aaataaaaag cccagtgcat
    31621 gctgcgagct gcaattttct ctaacagggg aatagccaat tccggtaatt gattagtcaa
    31681 acgccaaatc aatctgaaaa aagccaaaaa caataccaga aatccgtact ccttatgcca
    31741 accatataat tttaatttct caagactaat agggatcctg accatgtata aacccactcc
    31801 caatagccca ataattaaag cggctataat ccaatggaaa aaaatcgcaa ccagaccaaa
    31861 gctggttgaa gtattcctta attgcataga ttatccatat ttcttatcaa gataggcttc
    31921 tgcttctatt tctatttcaa catcatctcc tacttctggt attaaagctt ttattccata
    31981 atcagaacgc tttaatgaag tggtagcact aaacccagct gtttctctgt catttaataa
    32041 attaattcct gctttattca atatcacgtt caatatgatg ggttttgtga cgccatgcaa
    32101 agtaagcatt ccatacactt ttgctttatt ctttcccgtt acttccactc tgttactagc
    32161 aaaagttgct gttggaaact gcttggtatc aaaaaataac ttacctttca aatgcttatc
    32221 caactcgggt atacctgtga cgatgtcatc gaccttgata gtaacattca ctttactctg
    32281 ctgcgggtta tccttatcaa gaacaagttg accacttgca taccattttc ctgcttgagt
    32341 tgagaagcct aaatgcttga ccttccataa cacataagta tgctgattat ctaaggtcaa
    32401 ggtttgtggt tctgcgtaga cataagacgt caatgcaaag actgccaata tcgaagcaat
    32461 aaaatgcctg taaatccgtt tcatattcaa ctcccaaatt aaattaatgt tttatttacc
    32521 catatccaat gatccaccaa caagtttttc gcaaatccct ttgggtaata aaataaaagc
    32581 atccttttgg ctatccctgg ttgaagaccc agcacaatct gaagttgctg ttgaacaatc
    32641 attcatccct tttttaacca caccataaca tttctcctta ctcaaatcct ctgctattgc
    32701 tgtgtttgat gcaatgagaa ctaaaaaagc acttatggca gattgtatca atttatcctt
    32761 cattgacatg gataatctcc ttattatagt gaacttaacc agatttcagc ctataaacag
    32821 ataactctta agcttccctg gtatttttaa taaagtaaac tttattaaat cttcaatcat
    32881 cagcctttct ttaatgaata aatgaatgca aataatcata tgcttttttg gcgtattcca
    32941 aaggatgtgc tttttccgga tcctgctccg cctccactat catccatcca ttatagcgat
    33001 gtttttgcag ccctgcccat ataggtatga aatcaattgc tccatcacca ggcacagtaa
    33061 atacccctct tcttactgca tccataaagc tgagtctctc catttcaact cgtctcaaga
    33121 cctcagggcg aatgtctttc agatgaacat acaaaatacg atcaccgtac ttatttatta
    33181 aattaaccgg atctattcct gaaaagacag catgtcctgt gtcaagtaat agagaaagca
    33241 actttggact ggtattggtc attaaaaaat caatgtcttt ctcccaaaac accccagtcc
    33301 ctgcatggta atgataaacg atcttcatat tgaagtcgta agcaattcgg cctaaggtat
    33361 gcagcccttg aatcaataaa ctccattgct cttgagtaaa ttgagggata tgagcactta
    33421 atatcggtaa atcagtgcct tgaatagcat gaccacattc acaaacatta atatacgacg
    33481 cacctaatgc acgcataaaa cttaagtgct caagaaatct tgatacggtt ttttcaaaag
    33541 cattggcctc agtaaaataa gtgctaaacc atgaactgga taattgtaaa cctctttgtt
    33601 ccaaagcagc ttttaatgtt gttgcatctc gtgggtattt attccccagc tcagtgccag
    33661 aataacctgc caaagccatt tcgttaatac attgctcaaa actaatgtct ttacctaaat
    33721 tcggatcatc atcattgcac caattaattg gcgcgatacc aagttgaata ctcatcttca
    33781 cttccatctt caatattgtc taactgtttc aatttgctct tgcatcattt ggtaagcttt
    33841 actcactgat tctgattgag atacctcagc aactccaacc cgccaccagg tcttatatcc
    33901 ttgactcatg gtctttggta acacaggaag aacaataacc gtactatttt cctgtttttt
    33961 tgctgagtct aaagcagtcc agaattgctc ataggattta acaaaataag tagctgcccc
    34021 caatccctct gcgtatttac aaaaatctat gggcaaataa tcgccactta aagcattagt
    34081 agttggctct ctataacgaa attcattacc aaaacccaga ctaccattcc cttcctgtaa
    34141 atttctaatg cactgaaatc catgattatc aaaaatgaga attgtaattt tcttgcgttc
    34201 ctgaatggcg gttaaaagct ctgaatgcat catgataaaa ctgccatccc caactaaaac
    34261 gtagacttct ccttcctgat tttcttttgc aatacgtact cctagacccg ctgcaacctc
    34321 atatcccata caggaataag cgtactctaa atgataatcc tttgcccttt tggattgcca
    34381 aattcgatgc aaatcacctg gcaaactccc cgctgcacta attatcacat cctcttcagt
    34441 aacatattga ttgagtagtc ccagaacagc agtttggtgt aacccagcct gctctgtaga
    34501 ataagattga gtcaatttat ctaactcttg ggcccattca ttttgataat gacatattaa
    34561 ctgctggtaa gagagggagg tttgataatc tgccagctct ttccccaatt gctctaaacc
    34621 agttttagca tctcctttta acataactcc attcattttc atggcatcaa gacgtgatac
    34681 attaagatga atgatttggc agtcctggtt tttaaaagcc catttggaag ctgtcgtaaa
    34741 atcctgtaat cgagacccga taaccaaaat aacgtcagcc tgagctgcca gagaattcgc
    34801 tatttcagat ccagtaacac ctattccacc aacgttcatg gggtgtttcg ctgccaggct
    34861 actttttcct gcttgggttt cagcaacagg aatgcgatgt tttaatgcaa actctgataa
    34921 agtctccgaa gccagtgaat aatgcacacc accacctgca atgattaatg gttgcttagc
    34981 ctttctgata tgccgtatgg cctctgccaa cattgcatca cttatcctct ctctctcaat
    35041 acgccataag cgctttttaa aaaaagattc ctcatattca taagcttcag actgaacatc
    35101 ttgaggcaaa cacaaagtaa cagtacctgt ttcaactgga tcggttaata ccctcatagc
    35161 atgcatacac gctgtcatta actgttcagg tctggttatc ctgtcccaaa acttacttac
    35221 tggtttaaag caatcattaa cagatatggt gtagtcatat ggcacctcca attgttggag
    35281 aaccggatct ggttgcctgc aactgaatac atcacctggc aataatagta atggaattct
    35341 attggttgtc gccgtcgccg ctgcagtaat catattagta gcgccaggac caattgatga
    35401 agtacaagcc attatcccta gccgattttt ttgtttggca taggcggttg ctgcgtgagc
    35461 cattccttgt tcattatgtc cctgataata aattaaaccc tgagcatcgt attccaatgc
    35521 ctcacctaat ccggttacat taccatggcc aaatattcca aatatgccat tgatgaaacg
    35581 gtattcatta ttatccagaa cgacatactg attagctaaa aatcttaata gggcctgagc
    35641 catagttaat ttaatcttca tttatttaac tctcaaaatc atactgcaga taaggattac
    35701 tactgtttct gttgccatgc tcttaaattt gccctgcttg tccttgtcca ttcatggtct
    35761 tttatgaagg taggcaaggt atatgcgtta ttatttaaat gccggataaa ccacagtgta
    35821 taaagtgcat aaccgggcgc tgcacaatga gcatgttcct gattattggc aatctggtag
    35881 gtatcatagt gttcaattcg catcacctct ttaccatttt cgccaaaagc atagccttga
    35941 ggctctgaaa acctgtaatg gtaaatttca ggttgttgat gataatgtga aggataactt
    36001 gaccaacgcc cttgaaacgt aataatttcc ccaactacca aattagattc gggtctgttt
    36061 cgtttatcaa ataccgttct gacaagtcga tatgaagtat tttcaagaac tccttctccc
    36121 ctattgtcta gctcaagcat attggatgca tcaaataaaa tcggagaaaa taaattttcg
    36181 ttttccgttt ccattaataa aatttcacaa tcagtcagag cgattactga acttacttca
    36241 ttctttgagc agtgcaagac atacggtgat tgagaaaaat aatctgtacg ttcaacctcc
    36301 tggctataag agccatactg gaaaacaact tttcctgtca ttaagagggc tgcaaattca
    36361 taacttgagt taaattgatg gctttgcccc ttatttaatt taagagaact aaaactcatt
    36421 gctgtaggtg aatcttgcat cgttacaatc gaatttaaac catcggcaaa tccacatgga
    36481 tttttaataa gcaactgtgt cttttgattt ttacttaatt tctcatgcag ttgtgttaac
    36541 tgagagctgc tccatatttc aggatcatgc tcatattctt gaataaaata gtccaattct
    36601 tccttgtatg gaattgcagg agcgcaacca tgacgagtca cgaccaaggc accacaagca
    36661 ttagcatagg ttactgcttg ctcccatgat ttcccttgca gcaaaccaga taaaaggccg
    36721 gacataaaac catcacctgc tcccaaaaca ttcaatactt caacaggaaa agattttgcc
    36781 tgtaatagac ctttgtctgt tccaaaatga acacgagcac ccttctctcc taatttaaca
    36841 actattggcg catcggttcg ctgcctgata ttgattaatg aaacttcaat atcattcgaa
    36901 ccgcctgcaa tacatatttc ctcttcagtc ccaacaatca aatcgcataa aggcaaaact
    36961 tgttgataca cttgagttac atattcattc tggcaataac gactttcccc attaccaatc
    37021 acagttaacc gccaaaggac aggtctataa tcgagatcaa aaactattgt cgtttctgct
    37081 tttcgggcta aatgaactac ttccagggta gtatttttca ctgattctgt ggataaacca
    37141 gtgcccgtta tcaataatgc ctttgcattt tttaaatcag taaaactaat ttgatctgat
    37201 ttaatctgca tatctgcgca atcattacga taaaaaagca gagggaaatt ggagggaggc
    37261 ttaatgccca aaagtacaag gccagttaaa tgattatctg tttccgataa taactcaata
    37321 ttaactccct cacgttccaa ttcttctttc agaaacttac ctaactcatc tgagccaaca
    37381 caggaaaaca tctggctttt taatcctaat cgggcagctc caacggcaat atttcctgca
    37441 caaccaccca aatatttttt aaagcttgca acatccttca aatccaatcc tatttgctcc
    37501 gcgtagagat caactgctac gcgtcccata caaatcaaat cgattgctct attcttatca
    37561 aaataaaatc cgggaaactg tttcatagcg gcctcaactc aatactaccg ggagatgcgt
    37621 ttctaaagat tgtttcgctg cttttgcaat gcgcaaggct tgtagtccat ccaatcctga
    37681 aacgggactt gtctgattat cattccaggc agtataaaac gcattgagtt ctgccagata
    37741 agcttgatga taacgctcaa ggaaaaaata ttgaggtttt gcgccatccg ttccattagc
    37801 cgagtatcga gataccgtat gctccaattg attttcagcc aataacatac cagctgaacc
    37861 aaatacttct atcctttgat catacccata gattgcttga cggctgttat ctatgacacc
    37921 aaatgcgcca ttagcgaacc gtagttgaat gattgctgta tctacatcat caaactgctc
    37981 aaaatcagga ttaattagaa cagcccctgt tgcataaacc tccacaacct cggattgcat
    38041 taaaaaacgt gccatatcaa aatcatgaat agacatgtcc ataaatattc caccagatgt
    38101 cgcgcaatat tctttggatg gacaagccgg atcacgagaa gtaattcgaa gaatatgggg
    38161 agtgccaatt tcaccaaact gaactctttt ctgaatgctg gcaaaacttg ggtcaaatcg
    38221 acgattgaat ccaagttgca ataaagtatt attgtttttt attatattta atgttttctc
    38281 tatctcttct tcattcaaac caatcggttt ttcacaaaaa accgctttct catgaatact
    38341 cgctgtaata atttgttcga ggtgtaaatg agatggtgaa gcgatcaata tggcatccaa
    38401 ttcagggtgg catagcacat cttctgattg attagacacg attgcaatcc ctaatttttc
    38461 tgcccaattc ctatcgatct ggggatctgc tatggccact aattcaaatt gcggtaaatg
    38521 aaatagaata ttttctgcgt gcagtctacc aattcttcct gcacctacta tcccaattcg
    38581 acatgttttc tttttcatga ttaatccata attaaattaa attttgctat ttttatattt
    38641 ttcgatactg gttgacctat atctcttatt ttttttccag agcttaaatt gtcttccagt
    38701 ttttccagcg ataccgatgt ggtttccggg acaaaataat aaataaacca gcatgccagc
    38761 aaacaaaagg tagcaaacat agcaaaggta actgtttggc ctgagaattg ataaatggga
    38821 agaaacaaaa gtgatacaac aaaattagcg ccccattgaa acactgtggc aatgctcatt
    38881 gccaaaccac gcactgataa gggatatatt tcggatatta aaacccagaa cagagacccc
    38941 aggctaatgc aataccccat tacataaaat gataagccgc ctaaaatcca gaatttattg
    39001 acgagtaagg agttgctgga taataagcta acaagataca agctaattga tgcgatcaaa
    39061 gtaccactga ttaacaaaaa ccttctgcct aatttatcaa cataaaaaag ggtaaccact
    39121 gtaaaaataa aattgaccag acccaaaaaa aaggtagcga gaattgcatt tttaactgga
    39181 gaaaacccaa atgattcaaa gacaacaggt ccataataca tcaaggcatt gatcccggaa
    39241 aattgctgga atattcctaa accaactcct aataagagaa caaaaacaat tggtttttta
    39301 aacatctgga tataggctgg tgcaggatta actaaattat tactaatttc agtcaattcc
    39361 tgctgaatat tatatccaac tggtctaatt tgctttaaag tctttaaagt agcatcagct
    39421 ccatatttca tcattatcca gcggggtgaa tgagggacaa aatacattcc tgaaaataaa
    39481 acgagagcag gtagactccc tatccaaaat aaatatctcc aactcattaa agaataatca
    39541 tggagaaaat agccaattaa ataggcgata gcctgaccaa acgtgatggt tagtccatta
    39601 atcaatacta aagttcccct ctgatgaggc ggcgcgattt ctgcaatgaa taaaggagca
    39661 atataggagg caatacctat gcaaatgccg ataataaatc gtccgagtaa tatcgaaacg
    39721 aagccgggag ccaaagcaca taaactactc cccagtataa aacctaaagc tactactttt
    39781 aataaggatc gtctgcttaa cttatctgcg acaagaccac ttatgggtat tcccaatata
    39841 catcccaata aactactgct gacaacttgt gcccattgcc agtctgttag tgccaattgg
    39901 gagataacct gatctttcac atcagctatg acgctggaat caaacccgaa taaaaaaccg
    39961 ccaaatccgg caattaccgc gatcaggaat acaaatgcgt tcattccctt gttcatgtaa
    40021 ctatctgtcc ctaatgaaaa ttttgttcgt gcatgatgtt atcacggtca ggttgcgaga
    40081 aaaatatcaa agtgtaaatt attgaagata aaataatact ttaccccgat aatggaggtt
    40141 tgctgattaa ataaacactt ttgggcggga aaaggacacg cttagccaac gttccaattt
    40201 aaatcaccat aagtagttaa ttcatgcaac aaagggtgca acaccctatg ccacgcaaac
    40261 tcttgctgca ctgcaatcac aagttgcatg aattaattat actagagatg cagagatttc
    40321 tagaggtatt gttgcccatt agaaatgggc cgttaaagtt aatttggtaa tttttgcaat
    40381 attgctgcaa aaggcaagga ccctaaagtc ctacctatct gccgtttgaa cacaaaaata
    40441 attatcgtca ctcaactagc aacgtactta tgggcgcaaa ttgccatcac agtattccac
    40501 atccatttca ttagggctat gaaatgaatc tgatacctga aaagcctctt gtggcctgta
    40561 attaaaaaat cccatctctg aagcagtggc aattacagga gcttgagctt ctttatacca
    40621 ggatgaaaat tgatcttttg tagagctagg tttattttgt aagtatctta tatgttgcgt
    40681 tagctcctga acaatttgca gatcactttc tataggtgat ttaattgtat tcaggatttt
    40741 aatggcttgt tgataagtag ctattgcatc tgaaatcttt tgattttgta tataagattt
    40801 ggctagattt agaacttgca ttgccatgcc gcgataagag ctatcactag gcgttacatt
    40861 tacaaaaatc gggttaaaaa taactctatc aactccagag attgaattag ggtttggggg
    40921 ttctgctgcc aaagacaatt gcgataacat attttccaag ctacgatcaa tttgttgtat
    40981 ttcactaatc gatatatttt caaactgctc aaaaagcgct tgctcctgct gccttgcaac
    41041 ttgcccctcg ccgagcagat attcatgatt cataaatttt gcggagttta aataagactt
    41101 tgttgatagt tttttttcat aaaaaatatg aaattgtttg aaattagcaa ctggatcccc
    41161 aatttgttgc tcttcaaatg ttttattttg aatcaa
//
LOCUS       DAXLDT010000009        40595 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXLDT010000009 DAXLDT010000000
VERSION     DAXLDT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 40595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40595
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.009"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1975
                     /locus_tag="ACF2SP_000395"
     CDS             <1..1975
                     /locus_tag="ACF2SP_000395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFD2358205.1"
                     /translation="NYRPDIDGLRAIAILLVLIYHGGLSLFPSGFIGVDVFFVISGFL
                     ITTIIHESLNKNDFSFFDFYNRRLWRLQPVFVALIVVTTLLSVLFYLPDDLIEFSRSA
                     RKASLFISNLFFNKTTTGYFSPDTHQLPLLHTWSLSIEWQCYLVLPLVMYGLYRFLNK
                     GQVIYVVHGLAILFFLLALHYSQAAPAQTYYQFSSRIFEFLIGSCIALSPRIEFSINK
                     SILVILGSSALFFIIYTATLDNILLGYPNWHTLFVCLATGLLIALGTNFPSLAIVNAL
                     SFRPLVFIGVLSYSLYIWHWVLFSILRYQNIEERSEVLFIVYGIVFVLAYLSWRYIEK
                     PTKKFRHIPFRYTVVILLLIPVLFSHLSSLVIKSHSGYPQRFNQELVRVYKQLKSYES
                     PYRALCIDNTKADNAWKCKIGAKEQGGRTGLMIGDSFSNHYWGFMDVLGKAAGVSILA
                     QGTSSCISLPGIYLYDWWHFKDKIYQDCYEQTEKYYRMIQENHYDYVIIGQVWENYVS
                     AHIINQRGDDYSAELTKKRLADALDRALGVIISSGAKPVLIESTALTHGNLHQCFFKH
                     IKLRQSYNSEECRFTLTSSDNETWLNQLFDNLRRKYPQLIIIDPKQVQCRDNVCYSDL
                     NGIPVYRDEGHITDYASYQLGYLYLREFENPLI"
     gene            complement(2026..2292)
                     /gene="yhbY"
                     /locus_tag="ACF2SP_000396"
     CDS             complement(2026..2292)
                     /gene="yhbY"
                     /locus_tag="ACF2SP_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFD2358206.1"
                     /translation="MDTSFKQSLKAKAHHLKPVVLLGAKGLTEAILAETNVALLAHEL
                     IKVKINGAEKEDRMQMAEELCEQLHAELVQMIGNTLVLYRKNED"
     gene            2395..3015
                     /gene="rlmE"
                     /locus_tag="ACF2SP_000397"
     CDS             2395..3015
                     /gene="rlmE"
                     /locus_tag="ACF2SP_000397"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFD2358207.1"
                     /translation="MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKES
                     LIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVALDILPMDALPNVEFILGDFREDNV
                     LQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
                     KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL"
     gene            3129..5048
                     /gene="ftsH"
                     /locus_tag="ACF2SP_000398"
     CDS             3129..5048
                     /gene="ftsH"
                     /locus_tag="ACF2SP_000398"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFD2358208.1"
                     /translation="MNDMVKNLFLWLIIAIVLVSVFSNFGPRNSVAEKISYSQFLQEV
                     DQGMINSVTIEDNKIIKGVTKNNKRFVTYMPMQDNALLGELLKSKVDVSGQEKQQESF
                     LLHLFINWFPMLLLIGVWIFFMRQMQGGGGRGAMSFGRSRARLLGEDQVKVTFADVAG
                     VDEAKEEVKELVDFLRDPTKFQNLGGRIPRGVLLVGSPGTGKTLLARAVAGEAKVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDERE
                     QTLNQLLVEMDGFEGNEGVIVVAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQI
                     LKVHLQKVPVDSHVEVKAIARGTPGFSGADLANLVNEAALFAARANKRKVGMIELDKA
                     KDKIMMGAERRSMVMDDNEKKLTAYHEAGHAIVGLSVPEHDPVYKVSIIPRGRALGVT
                     MFLPEQDRYSHSKRRLESQLCSLFGGRIAEELIFGPESVTTGASNDIMRSTEIARKMV
                     TTWGLSALGPLTFGEEEEEIFLGRSVNKHKEMSDRTAQQIDDEVRAIIDRNYQRAKEI
                     LETNIDKLHLMAQSLIKYETIDTNQIQEIMSGKEPTPPEDWGSTKPMDKAESVNDAPA
                     KPINGETIENPAEGH"
     gene            5081..5956
                     /gene="folP"
                     /locus_tag="ACF2SP_000399"
     CDS             5081..5956
                     /gene="folP"
                     /locus_tag="ACF2SP_000399"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFD2358209.1"
                     /translation="MNSEQFLGWLERKSQPPSSLVFEKPLIMGVLNVTSDSFYDGGKY
                     LSVDRACDQALKLIACGADLIDIGGESTKPGAPPVPLDVELSRVLPVIKQLRALADVC
                     ISIDTNKPEVMEAAIDAGANVINDVYALRKDGALNVAAQLDVPVCLMHMKGEPRTMQE
                     NPSYPEGIMKELQHFFEERIDKCQKAGLNKNKLILDPGFGFGKRVKDNLKLIYHLNEL
                     RSFGLPILLGVSRKSTIGAVLNNEVEQRLIGSITLGIYAALKGTAIIRTHDVDETNQA
                     LTMINAIYQAAKEIS"
     gene            6020..7387
                     /gene="glmM"
                     /locus_tag="ACF2SP_000400"
     CDS             6020..7387
                     /gene="glmM"
                     /locus_tag="ACF2SP_000400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFD2358210.1"
                     /translation="MSQRKYFGTDGIRGHVGLSNINPEFVLKLGWAVGCVLANGARKK
                     VVIGKDTRVSGYMLESALEAGLSAAGVDVALLGPMPTPGIAYLTQTLRANAGIVISAS
                     HNLFEDNGIKFFSADGGKLPDSVELAIEAQLEKQLQTVPSAKLGKATRINDAAGRYIE
                     FCKSTIPSLSRLSNLKIVVDCANGATYHIAPNVFSELGADVVPIGIKPDGFNINQECG
                     STAPELLREKVIAVGADIGIGLDGDGDRVILVDSLGNLVDGDQIIYIIAKDRHQRGVL
                     HGGVVGTLMSNYGLELAITSLGVPFQRSKVGDRYVLETLREKDWKIGGETSGHIVCLD
                     KTTTGDGIVAALQVLSIMVKQNKALHELTAGIQLLPQTLVNLKTNNAALLASNPDVIQ
                     AVKNLEKHLNGEGRVLLRPSGTEPLLRVMVEGANASIVKQQAQMLCDDISQIDKKMTE
                     SLPST"
     gene            complement(7457..10816)
                     /gene="lepA"
                     /locus_tag="ACF2SP_000401"
     CDS             complement(7457..10816)
                     /gene="lepA"
                     /locus_tag="ACF2SP_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LepA"
                     /protein_id="HFD2358211.1"
                     /translation="MNEIILHLMNEISALTPPINPLNLKQIFSSTLLPEGKAPVEVES
                     QLNLLLLIKKHVDEYSSLAEEQDEYKRKKQKQDEVMKKGEELQRKISDLAKKIELAKS
                     ELNPLYKEIEPKANEVLSKQNPLAPPYKFPEYGEVQKSIVVGHYSYLFKTSKEIETTR
                     AKLKLVLLKNKWHALMTDLSQLGIQPPVEEDAVDSLQKFTAAVELEIKKLTRSCEKWD
                     ELQKRFAKQLSATDINKKETELEKQIELLLKDLGRIEATVTNHSLVPDLKAELIRKFQ
                     ESEDPQGLIQEYQNQIDGILSNFNPSSWLSWYSDSSYSDKQQKLKDSVSFLQLLAQQK
                     ELKIKHHELVKQQELLIKAIPETRSNEEDISIYKKLVPDAIDLINEIPIQSIPNFVLP
                     SGLSYDSSPADFYLVLLILMPKVSEKKEQYSQALEKLQEMPALGKQIHQLRSGYDLPA
                     TMDVQLPSLQEAEENKDTLNTEDPLKEELQSQIKLCQSYLETAKQIDLLMKNHGQTTN
                     EAKALIEQLRLISNQPLKNEDINSIKEDLSTLANQIQSLVSELNQLPLPNLTGEKIDP
                     SLEKQEIAVDKAIPITVELMHPAQVFQIGNDSHLEKRQQDNEEETPVVLESSRPHQVI
                     HQEPESLVELGQIDNGHEIPVVLESTHPPQIIHTESESAGKKQRVEVEQEIPVVLELT
                     RPSSMVEKDSGVTVEKLEVAERQDTPIVLESTRSTPMVQKDSGLSGEKQHKEIEQEIP
                     AVPELTLRPQVVQQGSESSEEQQVPNGQETLAVVKSSPSEPLRPSTPEVPVISRKPNG
                     LSLFNGHDELSEDNILAFFDEAGNQITISSEEDSETLAIDRGKVNSLGIDKAFKEEPV
                     STGHELDKLSNPQHQESAPNISPLPPSSILLKQKLDSFHVQNMEYIKQQSEEIQLWYK
                     GLYDAAQSSCLNEALGLKALHLFKDILFELKNQNDLSVLLAYKRMCPNPLQDIQNILR
                     LKPALPILDESIDEEQQLKNWPEELQKFHQQYVKLKKEHPLEAELFIQATHSLISIKH
                     LMELPDAKTSSREAMPLITQDPRYEPLKRHRGFIKAWEYIEDFFRMLIGKLTGQDEYE
                     YSKRPCFFKTRSHRLLEEADTMLHSMAPTSS"
     gene            11060..11809
                     /gene="tpiA"
                     /locus_tag="ACF2SP_000402"
     CDS             11060..11809
                     /gene="tpiA"
                     /locus_tag="ACF2SP_000402"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFD2358212.1"
                     /translation="MRQKIVAGNWKMNGQIQQVTELVSQIEELIGFDCAAQVAVMPPS
                     IYIPKVRDCLKTGKVVVGAQNVYPKDYGAYTGELSAPMLKDFDCRYVLVGHSERRQFF
                     HEDENFVAQKFHHVKDHGMIPILCVGETLSERENGKTEQVIAQQVLAVSAKGKDCFRD
                     CVVAYEPVWAIGTGKTATPEQAQKIHQFIRDLVGEINDSDAKHLTLIYGGSVNENNAK
                     ALFSMPDIDGGLVGGASLNAKQFVEIVKCIN"
     gene            11797..12120
                     /gene="secG"
                     /locus_tag="ACF2SP_000403"
     CDS             11797..12120
                     /gene="secG"
                     /locus_tag="ACF2SP_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFD2358213.1"
                     /translation="MYQLILMIHVLIAVILIGLVLIQHGKGADIGAAFGSGASNTLFG
                     SQGTGGFLFKLTGGLAMTFFITSLMLSYMVSTQNQKAGQLAIPQQTGAPVSSVPVPVE
                     KNNKK"
     gene            12191..12275
                     /locus_tag="ACF2SP_000404"
     tRNA            12191..12275
                     /locus_tag="ACF2SP_000404"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12225..12227,aa:Leu,seq:gag)
     gene            12300..12656
                     /locus_tag="ACF2SP_000405"
     CDS             12300..12656
                     /locus_tag="ACF2SP_000405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFD2358214.1"
                     /translation="MLSQYLPVLVFIIVALILGAVMIGAGSLLSKHSPNSAKNSPYEC
                     GFGAFESSHIPFDVRFYLVAILFIIFDLETAFFFPWALALRKIGWFGFYAMMLFLGLL
                     VIGFIYEWKRGALEWE"
     gene            12903..13379
                     /locus_tag="ACF2SP_000406"
     CDS             12903..13379
                     /locus_tag="ACF2SP_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFD2358215.1"
                     /translation="MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMH
                     VGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA"
     gene            13402..14085
                     /locus_tag="ACF2SP_000407"
     CDS             13402..14085
                     /locus_tag="ACF2SP_000407"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFD2358216.1"
                     /translation="MTKNEYLIEKLQANLANHITELTSAYGEVTIECEVHNLLPVMIE
                     LRDREEFSFDQLIDLCGVDYLHYGDYDWETESATEHGFSRGVERQEAKAYAVNKPRFA
                     VVYHLLSTKKNHRLRVKLFVEESHLIVPSVHHLWKSANWFEREAYDLYGILFDGHPDL
                     RRLLTDYGFIGHPFRKDFPLSGEVEMRYDAKLQKVIYAPVDIVPRIVVPKVIRNDNRY
                     IGNEGSKND"
     gene            14063..15331
                     /locus_tag="ACF2SP_000408"
     CDS             14063..15331
                     /locus_tag="ACF2SP_000408"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HFD2358217.1"
                     /translation="MKVAKMIELKNYTLNFGPQHPAAHGVLRLVLELEGETIVRADPH
                     IGLLHRATEKLAETKPYIQSIGYMDRLDYVSMMCNEHAYVMAIEKLLGIEPPLRAKYI
                     RTMFDEVTRILNHLLWLGATALDIGAMTVFLYCFREREDLFDCYEAVSGARMHATYYR
                     PGGVARDLPDTMPQYKPSRWHNEREIEKKNHNRQGSLLDFLWAFTERFPRCVDEYETL
                     LTDNRIWKQRTVDIGIVSPENALQWGFTGPMLRGSGIAWDLRKKQSYAAYDRVDFDIP
                     VGKTGDCYDRYLVRVEELRQSNRIIRQCIEWLRKHPGPVKVDDYKVTPPRRVVMKHDM
                     EALIHHFKLFTEGFCLPRGEVYSSVEAPKGEFGIYMVSDGANKPYRLKIRAPGFAHLS
                     SFDDMVRGHMLADGVAILASQDIVFGEIDR"
     gene            15392..15895
                     /gene="nuoE"
                     /locus_tag="ACF2SP_000409"
     CDS             15392..15895
                     /gene="nuoE"
                     /locus_tag="ACF2SP_000409"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFD2358218.1"
                     /translation="MSANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIV
                     QEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRD
                     SAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
                     KVLEQYQ"
     gene            15914..17191
                     /gene="nuoF"
                     /locus_tag="ACF2SP_000410"
     CDS             15914..17191
                     /gene="nuoF"
                     /locus_tag="ACF2SP_000410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFD2358219.1"
                     /translation="MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQL
                     IIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLN
                     PHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
                     FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFAS
                     VPVIMEKGGEWFLKLGKPNNGGAKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIK
                     GRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALY
                     RISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICA
                     LGEAAAWPVQSFVKHFYDEFEYFIKHKRSIVAA"
     gene            17208..19559
                     /gene="nuoG"
                     /locus_tag="ACF2SP_000411"
     CDS             17208..19559
                     /gene="nuoG"
                     /locus_tag="ACF2SP_000411"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFD2358220.1"
                     /translation="MATIEIDGKTFEAENGKMIIEVADEAGIYIPRFCYHKKLSVAAN
                     CRMCLVEVENGRKPVPACATPITNGMKVFTKSEQAIHSQKVVMEFLLINHPLDCPICD
                     QGGECELQDISMGFGADKSEYTESKRAVDDENLGTLISTEMTRCIHCTRCVRFGEEIA
                     GVRELGATGRGEKTQIGTYVEHSMTSEVSGNIIDLCPVGALTSKPYRFKARAWELTQH
                     DSVAPHDCLGSNVHIHTRNNKLLRVVPRENESINETWLSDRDRFSYLGLNATSRASKP
                     QIKKNGQWEVVDWETALKFAAEGISRVIKQHGPEQMAAFASSSSTLEEMYLLQKLMRE
                     LGVQNLDHRLQQIDFRDQAFLPTTPVSTLPYAEIDQQHSILLIGCNIHREVPLAGTRV
                     RKAFRNGAKIYALNPVDFDYHFDLSGRVVISPLEMPMQLAKLALALTSELASLPEEVQ
                     KLLIGLEVDKQTKQIAQSLKEEKACLITGAIVENHPEASLLRTLVAIVQKLSGAKLVR
                     LTTGANSAGACIAGMLPHRTVAGKSIAEPGLNVQEALNSKLKGYLLMGVEPGYDFANP
                     AGARQSMLAAEFVVLLSAYEHESMHDYADVILPIAPYAETSGTYINIDNTWQTVKGAM
                     LPLGESRPAWKVLRVLGNLLHCKKFDYTSTEDILEEVKEAVSMTMEHEYEPYYPESLP
                     VINQSLVRVGEWPLYRIDAITRNAKELQLCAASESACIRIHPSTADRLKLEEIATVSQ
                     GDIEITLPLKRDERIAVDVVWVANAMPETVDLGHSFAAITIKR"
     gene            19572..20594
                     /gene="nuoH"
                     /locus_tag="ACF2SP_000412"
     CDS             19572..20594
                     /gene="nuoH"
                     /locus_tag="ACF2SP_000412"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFD2358221.1"
                     /translation="MLHTIGILIWIIIKILVIVVPLLLSVAYLTYAERKVIGYIQVRI
                     GPNRVGLKGLLQPFADLLKLITKEIIVPTRSNKYLFVIAPLFALVPSLVGWAVIPFQE
                     GVVLANINAGVLYLFAMSSLGVYGVLIAGWASNSKYAMFGALRSTAQTVSYEIAMGFA
                     LVGVLLAAGSMNLTDIVNSQKGGMLHWWFIPLLPLFLVFWIAGIAETNRAPFDLAEGE
                     SEIVAGFHVEYSGIGFALFFLSEYASMILISTFMAILFMGGWLSPFEGITFLDQIFFV
                     VPGFVWLLLKISFFLFVYLWVRATFPRYRYDQLMRLGWKVLIPVTIVWLVVTSLMVVA
                     HVKPWF"
     gene            20613..21113
                     /gene="nuoI"
                     /locus_tag="ACF2SP_000413"
     CDS             20613..21113
                     /gene="nuoI"
                     /locus_tag="ACF2SP_000413"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFD2358222.1"
                     /translation="MKKVYHYIIHYVRTYLLLELLAGLWLTVKYFFRKKITVQFPEEQ
                     TPLSPRFRGLLALRRYPNGEERCIACKLCEAVCPALAITIESEQREDGSRRTTRYDID
                     MFKCINCGLCEESCPVDSIVVTPIHHYHISERGQNIMTKEKLLAVGDLMETQLAADRA
                     ADEKYR"
     gene            21125..21784
                     /locus_tag="ACF2SP_000414"
     CDS             21125..21784
                     /locus_tag="ACF2SP_000414"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFD2358223.1"
                     /translation="MHEWVIQAIFYIFALLTVLSALMVISQNNPVRCVLFLVLTFFTS
                     AVLWVLAEAEFLALILILVYVGAVMTLFLFVVMMLNIDIESMKSHLIKYLPFGLIIVA
                     LLTGLLMVAIPKDLFKNSVQIQEKPLSVSSQLMDLSTQDELVPPENATSNTEKLGMVL
                     YTDYILAFEIAAVILLVAIVSAITLVHRGAIRSKRQDITQQIMTRRAERVKLISMKSE
                     K"
     gene            21802..22107
                     /gene="nuoK"
                     /locus_tag="ACF2SP_000415"
     CDS             21802..22107
                     /gene="nuoK"
                     /locus_tag="ACF2SP_000415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFD2358224.1"
                     /translation="MIPVYDYLVLGVILFGLSLVGIMLNRKNIILLLVCVELMLLAVN
                     TNFIAFSHYYNEVGGQVFVFFILTVAAAEAAIGLAIVMLLYRNRGNIDVDKMNHLKG"
     gene            22112..24085
                     /gene="nuoL"
                     /locus_tag="ACF2SP_000416"
     CDS             22112..24085
                     /gene="nuoL"
                     /locus_tag="ACF2SP_000416"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFD2358225.1"
                     /translation="MSIQQVCLVIVLTPLVGSAIAGFFRNQIGRVGAHTVTILGVAIS
                     LVCSIFLAWGLFSGSIPNTNSLVYHWASGGALIPYEFNIGFLIDPLSVVMLVIVNFVS
                     LLVHVYSIGYMADDDGYQRFFSYISLFTFMMLMLVSANNFLQLFFGWEGVGLVSYLLI
                     GFWYQKESAIEGSLKAFLVNRVGDFGFVLGIGLIFAYAGSLDYEKVFTSANYLASQNI
                     ELFSGYSWSLITVICLLLFVGAMGKSAQVPLHVWLPESMEGPTPISALIHAATMVTAG
                     VFMIARISPLIELSTAALSTVLVIGATGALFTGILALVMNDIKRVVAYSTLSQLGYMM
                     VAMGASAYSAGMFHLLTHACFKALLFLGAGSVIIGMHHEQDMRKMGGLWNKMPITYVT
                     YVIGSLALCAFPPFAGFYSKDTIIEAAQLSQIPGSSYAYFCVTAGAMVTALYTFRSLF
                     MTFHGKPRMDEHTLSHLHESPWVVWLPLVLLAIPSIILGYVLYMPILFDTPTLLGSSI
                     FVLPEHNVLAELAHEVSSPFASAVHAIYSLPFWITVLGAVIAWVCYIAVPSIPGYLAR
                     YFSIIYSILLNKYGFDRFNELFFVKGARGLGIAFYKIGDQKLIDGAVVNGSGRLVRWF
                     SGKGRKIQSGYIYHYATVMVFGLLAFLCWLILD"
     gene            24101..25606
                     /locus_tag="ACF2SP_000417"
     CDS             24101..25606
                     /locus_tag="ACF2SP_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoM family protein"
                     /protein_id="HFD2358226.1"
                     /translation="MHYLLNLLIWLPILGGVFVLLTGDDKNPNVSRYLTLFTILLCLV
                     LCIPLVKGFDLQSFGMQFIEEIPWMPALGINYTLGIDGLSLLLIVLSIFTNLIVILAT
                     WDSISKKVSQYLSAFLIMQGLLVGIFSAMDAIVFYIFWEATLIPMYLIIGIWGSDNRV
                     YAAIKFFLYTFLGSVLMLASFLYMGYIAGSFKIETFYAIKLGMTAQTLIFIAFFLGFA
                     IKIPMFPVHTWLPDAHTEAPAGGSVVLAAILLKLGAYGFIRFSLPIVPDACSKYAPLM
                     VALSLFAVVYIGLIAIIQKDMKKLIAYSSISHMGFVTLGCFAIFAIARHGGLSNAGLI
                     LEGAIVVMISHAFVSSAMFAGVGFIYDRMHTRQLADFGGIVNTMPIFASFFMLFAMAN
                     AGLPGTSGFVGEFMVILGSIKAGFWVAFWAATTLIIGAAYTLWMYKRVIFGPVANKKV
                     AALKDISGFELSAYVLLAIMVIALGVYPKPMLEFVHQTVSHTLALADKSKL"
     gene            25660..27102
                     /gene="nuoN"
                     /locus_tag="ACF2SP_000418"
     CDS             25660..27102
                     /gene="nuoN"
                     /locus_tag="ACF2SP_000418"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFD2358227.1"
                     /translation="MMTLLENIHVAIPEMIILITACLALLSDLFFRHKLKSIAFYISC
                     VGIIVSAAVSFVFIGSYKLLIFNKLFISDDISHLMKLFIDITVLLSFVYSQNYLDERQ
                     MPTGDYYVLGLFSTLGMMILVSAHSLLSLYLGLELMSLPLYAMTAIRRTDSDASEAAM
                     KYFVMGAIASGMLLYGISLVYGATGKLDLLDVANAVAVNWQQQNTLFAFAMVFILAGV
                     GFKLAAVPFHMWAPDVYQGAPSSVTLFISTAPKIAALGMALRFLTIGLVDITMQWQQI
                     ILVMALLSTGIGNLLAVIQTRIKRLLAYSAISHIGYALFGVLAASSAGYAAALYYILV
                     YAVMSAAGFGLVVLMSNHGMEIDSIDDLKGLNKRNPWLAFMMLLVMFSMAGVPPTVGF
                     FTKLLVLKALVDVHMTWLAVVGLLFTVIGAYYYLRIVKIMYFDQPENEDIVRIGTGNT
                     IVYSLNCLSLLYLGIFPGALIAACINAFAN"
     gene            27168..27244
                     /locus_tag="ACF2SP_000419"
     tRNA            27168..27244
                     /locus_tag="ACF2SP_000419"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27202..27204,aa:Met,seq:cat)
     gene            27358..27801
                     /gene="rimP"
                     /locus_tag="ACF2SP_000420"
     CDS             27358..27801
                     /gene="rimP"
                     /locus_tag="ACF2SP_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041173832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFD2358228.1"
                     /translation="MINDDLIVLLEPIIKNMGYELWGCEYLSQGKHSLLRIYIDKPDG
                     IGIDDCQEVSKQVSAMLDVEDPIPGHYSLEISSPGIPRPLFSIWQYQRYLGYEIHVKT
                     FKPVNGKRKLSGIIVSASEDTIVLDINNEHQEILLSNIVKANLTV"
     gene            27813..29291
                     /gene="nusA"
                     /locus_tag="ACF2SP_000421"
     CDS             27813..29291
                     /gene="nusA"
                     /locus_tag="ACF2SP_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFD2358229.1"
                     /translation="MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIG
                     VRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSI
                     EFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNVIIDLGGKA
                     EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEI
                     GENIIDIKAAARDPGNRAKIAVKTNDGRIDPIGACVGMRGARVQAVSSELGGERVDII
                     LWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVAKEQLSQAIGRNGQNVRLASQLT
                     GWTLNVMTTEEFESKNQEESSKIVNLFTNALEIDEEIATLLVAHGFSSLEEVAYVPKE
                     ELLAIEEFDEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLA
                     SKGITTMEDLAEQSVDELMEIEGISEEKAGALIMKAREPWFQ"
     gene            29380..31986
                     /gene="infB"
                     /locus_tag="ACF2SP_000422"
     CDS             29380..31986
                     /gene="infB"
                     /locus_tag="ACF2SP_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFD2358230.1"
                     /translation="MADVTVKQLAQVVGIPVERLLNQLQEAGLSFTDDQQTVNEEQKR
                     ILLNHLKGSSNRDISAAPERITLRRKSMSQVTVGHDMHSGKTVNIEVRKKKTFIKRSA
                     IPEQAEIEEPVVPPVVEEPVHEEITVVSEVSAETPELKETEEHPVIEPVAELDETVKE
                     EEKISLEENTAESQDELTHANTDVIENLVDVVEEAIPASKKEEVKPEKVSKKKHLEQT
                     DSDISEFKKGKKKPKYHTFEHDEEEQELHRRGGRSKFKKKKGTEKSDKYREAEETLTH
                     GFALPTAPIVREVLIPETITVAELAKRMSVKAAEVIKVMMSLGAMATINQVIDQETSV
                     IVVEEMGHKPVIIKEDAVETGLGEAISKGTKTEGRAPVVTIMGHVDHGKTSLLDYIRR
                     TKVAAGEAGGITQHIGAYHVSTPKGNITFLDTPGHAAFTAMRARGAQATDIVILIVAA
                     DDGVKPQTIEAIQHAKAAKVPIIVAINKMDKPDADPERVMNELSVQEVIPEAWGGDTM
                     FVNISAKSGMGIDDLLDAILLQSEVLELKAVTDGAAKGVVIESRLDKGRGPVATVLVQ
                     SGTLHKGDILLAGFQYGRVRALVSDNGDLVDSAGPSIPVEVLGLSAIPHAGDEAVVVP
                     DEKKAREVALFRQGRFRDVKLARRQKTSIEGIMENMTATESKVLNIVLKADVQGSLEA
                     ISDALTKLSTDEVKVEVISSGVGGITESDVHLAIASNAILIGFNVRADGTAKRLAEQE
                     SVSIHYYSVIYDIVDQIKGALTGMLAPQFKEEIVGIAEVRDVFKSPKIGAIAGCMVIE
                     GVVKRNNPIRVLRSNVVIYEGTLESLRRFKDDVLEVRQGFECGIGVKNYNDVKPGDLI
                     EVFETVEIKRDL"
     gene            31990..32361
                     /gene="rbfA"
                     /locus_tag="ACF2SP_000423"
     CDS             31990..32361
                     /gene="rbfA"
                     /locus_tag="ACF2SP_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFD2358231.1"
                     /translation="MSNNFKRTDRIAEMIQRKLALIIPQEIKDPRLKGFVTISAVKVA
                     ADLGHAKIYFTVLNEDKSVVTNILNGAASYLRSALARSITLRTVPQLHFIYDESIEYG
                     QRLSRLIDEVNPPDSSSDDNN"
     gene            32348..33259
                     /gene="truB"
                     /locus_tag="ACF2SP_000424"
     CDS             32348..33259
                     /gene="truB"
                     /locus_tag="ACF2SP_000424"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFD2358232.1"
                     /translation="MTTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTG
                     SLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYT
                     VSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
                     EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDE
                     LQDMPLSQRLACLIPIDQAIQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYG
                     EQSQFIGIGQALIHGDIKAKRLVSFAL"
     gene            33407..33682
                     /gene="rpsO"
                     /locus_tag="ACF2SP_000425"
     CDS             33407..33682
                     /gene="rpsO"
                     /locus_tag="ACF2SP_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFD2358233.1"
                     /translation="MSLNSAEKAEIINEYKRGDKDTGSPEVQVSLITGRIKYLTDHFK
                     ENKKDFHSRRGLQELVNKRRKLLKYLKRNDQARYQTLIQNLGLRDSY"
     gene            33797..35986
                     /gene="pnp"
                     /locus_tag="ACF2SP_000426"
     CDS             33797..35986
                     /gene="pnp"
                     /locus_tag="ACF2SP_000426"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFD2358234.1"
                     /translation="MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVV
                     WKKDSGESNDFFPLTVNYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLF
                     PDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
                     LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKSVIKSI
                     EELAREVGKSKPEWKAPEIDTVLKARINDVARNEVEAAYLIKDKQQRYQRLDELREQT
                     ISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLE
                     RTHGSCLFTRGETQAIVVATLGNERDAQILDGISGETRDRFMLHYNFPPYSVGETGQV
                     GSPKRREIGHGRLAKRALMAVLPDTNEFPYVLRIVSEITESNGSSSMATVCGTSLALM
                     DAGVPLKAPVAGVAMGLIKEGDRYAVLTDILGDEDHLGDMDFKVAGTEKGITALQMDI
                     KISGITNEIMERALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAEDKIRT
                     IIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKMALEEAQKQIKALIAEIEVGQTY
                     QGKVSKIVDFGAFINLLPGKDGLLHISQICAERTQKVEEVLQEGQEIEVFVAGIDKQG
                     RVKLEWKDKPQAEAKEVEGASVSATFLTMEEQSEEINSGNKISEEEE"
     gene            36121..37212
                     /locus_tag="ACF2SP_000427"
     CDS             36121..37212
                     /locus_tag="ACF2SP_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HFD2358235.1"
                     /translation="MKLFSFFLVLISFASLGQALPLHLLKYPPGFSVSIYAAPVSDAR
                     EMALGSKDIVFVGSRSAGKVYAVIPDKKNSHGTRVLTIASGLNMPNGVAFHKGALYVA
                     EIGRILRFDNIESNLNNPPEPVVVTTKLPKEKAHGWRFISFGPDDKLYIGIGAPCNVC
                     LMEDTRYATIMRMNADEQDLEIYAKGIRNTVGFDWDPVHHNLWFTDNGRDWLGDDLPP
                     DELNVAPRKGMDFGFPYCYGKSTADPVYGKTHSCSEFTPAVFEFPAHVAPLGMSFYTG
                     KLFPPNYWGQIFISEHGSWNRSHKIGYQVTTVKIKNNHVTEVKPFISGWLQGEKAWGR
                     PVDTLVMPDGSLLISDDYANVIYRVSYQQ"
     gene            complement(37271..37810)
                     /gene="folE"
                     /locus_tag="ACF2SP_000428"
     CDS             complement(37271..37810)
                     /gene="folE"
                     /locus_tag="ACF2SP_000428"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFD2358236.1"
                     /translation="MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLD
                     EIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIV
                     DFYARRLQIQERLTGEIAHCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
                     LGEMRNNSSCRLEFLNLIR"
     gene            complement(37822..38163)
                     /locus_tag="ACF2SP_000429"
     CDS             complement(37822..38163)
                     /locus_tag="ACF2SP_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFD2358237.1"
                     /translation="MNCLFCKIAQGEIPATVVFEDKNILAFRDIRPQAPTHLLIIPKK
                     HIATINDVNDGDSELLANILIRAKKLAQAEGLSETGYRLVFNVNSGGGQEVYHIHLHL
                     LGGRQMTWPPG"
     gene            38293..38829
                     /gene="ppa"
                     /locus_tag="ACF2SP_000430"
     CDS             38293..38829
                     /gene="ppa"
                     /locus_tag="ACF2SP_000430"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFD2358238.1"
                     /translation="MSLMEIQSGRDVPNEVNVIIEIPMHGEPVKYEVDKKTGALFVDR
                     FMTTAMFYPTNYGYIPNTLSEDGDPVDVLVITPVPLISGAVISCRAVGMLKMTDESGV
                     DAKILAVPTTKLSKMYQSMQTYQDIPQHLLLSIEHFFKHYKDLEEGKWVKVEGWVGPD
                     AAREEITSSINRYNHTKK"
     gene            38979..40244
                     /locus_tag="ACF2SP_000431"
     CDS             38979..40244
                     /locus_tag="ACF2SP_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HFD2358239.1"
                     /translation="MQLPLSTLLVLLFFLICLSAFFSGSEIGMMSINRYKLRHLVKSN
                     DKQAIRVNSMLARPDKLLSVVLIGNTLANIVASTLATLIGQRFYGDAGVAIATALLTI
                     LILVFAEMTPKTLAAIYPQQVAFASSLPLSILQWIFSPLVHLISLISNGILRLFNISV
                     KRMQKEMLTGEELRSVVHEAGGLMPVEHKSMLISLLDLEQATVEDIMVPKADIVGIDL
                     DLPWIELLDQLETAQHTRLPIYHGSIDHLVGMIHVRNILNLALENKLDMENLLNSAEV
                     PYFIPEATPLNIQILNFRKMKRRSCFVVDEYGDILGLVTMEDILEEIVGEFTTDIADL
                     SRDITPQQDGSVIVDASITIRHLNRMMGWSLPLIGPRTLSGLIIEHLGYIPPPDSCLV
                     IDHYHIEILRVSDNMIRGVKMIKANKKRK"
BASE COUNT        11897 a         6962 c         9006 g        12730 t
ORIGIN      
        1 gaattataga cccgatatag atggattaag ggccatcgca attttattgg tcttaattta
       61 tcatgggggc ctctcgcttt ttccatcagg gtttatcggt gttgatgtat tttttgttat
      121 atcaggtttt ctcattacta ctattatcca tgaatcactg aataagaatg atttttcatt
      181 cttcgatttt tataatcgaa ggttatggcg attacagcct gttttcgttg ctctaatcgt
      241 ggtgacaacc ctactaagcg tattgtttta tttgcctgat gatttaattg aatttagtcg
      301 tagtgcgcgt aaagcatcac tgtttatttc caacttgttt tttaataaaa caaccactgg
      361 gtatttttca cccgataccc atcaacttcc tttgctgcat acttggtctc tttcaattga
      421 gtggcaatgt tatctagttt tgccactggt gatgtatggc ttatatcgat ttctaaataa
      481 agggcaggtt atctatgttg tgcatgggtt ggccatttta ttttttttgc tggctttgca
      541 ctactcacag gcggctcctg cccaaaccta ttatcaattt tccagtcgta tttttgagtt
      601 tttaatcggt tcttgcatcg ctttaagccc tcgaattgag ttctcaatca ataagtctat
      661 tttagttatt ttgggttcat cagcattatt ttttataatt tataccgcta cactggataa
      721 tattcttctt ggatatccta attggcatac actttttgtg tgtctggcaa cgggtttgtt
      781 gatagcatta ggtacaaatt ttccttcact tgctattgta aatgctctct cttttcgacc
      841 attagtgttt ataggagttt tatcttattc tctttatatt tggcactggg tcctcttttc
      901 aattttaagg taccaaaata tcgaagagcg gtctgaggtt ttatttatag tttatggcat
      961 tgtttttgtt ttagcttatt tatcctggcg atatatagaa aaaccgacga aaaaattcag
     1021 acatattcct tttcgttata cggttgtgat tttattgctg atccctgttt tattttctca
     1081 tttaagctct ttagttatta aatctcattc tggatatcca caacgattta atcaggagct
     1141 ggttagggtt tataaacaat taaagagtta cgaaagccca tacagagcgt tgtgtataga
     1201 taataccaag gcagacaatg cgtggaaatg caaaatagga gcaaaagagc aaggtggcag
     1261 gactggcctt atgataggtg attctttttc caatcattat tgggggttta tggatgtgtt
     1321 agggaaagct gccggtgttt caatattggc tcaaggaacc tcttcttgta tttccttacc
     1381 aggcatatat ctttatgatt ggtggcattt caaagataaa atatatcagg attgttatga
     1441 gcaaacggaa aaatattatc gcatgattca agagaatcat tacgattatg tgattatagg
     1501 ccaagtatgg gaaaattatg tttcagccca tattatcaat caacgtggcg atgattattc
     1561 tgcagaatta accaaaaagc gtttggcaga tgccttggac agagcacttg gcgtgataat
     1621 atcttctgga gcaaaacctg tattaatcga atcaaccgct ttaacgcatg gaaatttaca
     1681 tcaatgcttt tttaagcaca ttaaattaag acaaagttat aactcagaag agtgccgctt
     1741 tactttaacc agttcagata atgagacttg gctaaaccaa ttgtttgata acctgcgaag
     1801 aaaataccct caattgataa taatcgatcc taaacaagtt caatgtcgcg ataatgtttg
     1861 ctattccgat ttgaacggaa ttccagtata tcgcgatgaa gggcatatta ctgattacgc
     1921 ttcataccaa ttgggttatt tatatttgcg ggagtttgaa aatcctttga tttaaaaagg
     1981 ttggtttgct ttgattcccc agactacaaa acgattttac aaattttagt cttcattttt
     2041 acgatacaga accagtgtat ttcctatcat ctgaactaat tcggcatgca attgttcaca
     2101 aagctcctcg gccatttgca ttctgtcttc cttttcagct ccattaattt tgactttaat
     2161 taattcatgt gctaacaagg ctacattcgt ttctgccaat atagcttcag ttaagccttt
     2221 ggcgcctagg agcacaacgg gctttaaatg atgagccttg gctttaagtg attgtttaaa
     2281 agacgtatcc actgcgatat cccagataat aaaatggtaa attattgcac gttttgctgg
     2341 gaaaaggtag taaaattctg tatcatagga aaaattgttt tatctttatt cgttatgaat
     2401 cgtactaaaa gcagtaaacg ttggctgcaa gaacattttg atgacgttta tgtaaaaaaa
     2461 gcgcaggctg aaggatatcg aagtcgagcc gtatataaac tcaaagaaat agatgacaaa
     2521 gagtcactca ttaaaccagg catgacagtg gttgatttgg gagccgctcc tggtggctgg
     2581 acccaatacg cctcagaaaa gatgagagga agtggccgtc tcgttgcatt agatatttta
     2641 cccatggatg ctttgcctaa tgtggagttt atccttgggg attttcgtga agataatgtc
     2701 ttgcaagaat taattaattt gattcctcaa aggactttag acttgttatt atctgatatg
     2761 gccccaaata tgagtggaag ctcagcaata gatattccaa gagccatgta tcttgtagag
     2821 ctggcttttg atttcgcgga aaaaatgtta aaacccggtg gtaatatgct agttaaaatt
     2881 tttcatggct cgggttttga tgaattagtc aaacaggcac gagcgtcttt tgaaaaagtg
     2941 gtaatccgta agccatccgc ttcaagatct cgttcaaaag aaacctattt gctggcaaag
     3001 ggctataatt tatagtagtt ttatatatat ttataaatat gcttggcgtt gttttggatg
     3061 atatgagctg tgcaagagct attttgggtc tcgcaacaaa attgtaagta actaacgagg
     3121 ttaatacatt gaacgacatg gttaaaaatt tatttttgtg gctgataata gcgatagttc
     3181 ttgtctctgt tttcagtaat tttggtcctc gtaactcagt tgctgagaaa atttcttata
     3241 gccagttttt acaggaagtg gatcaaggga tgattaactc tgttacgatt gaggataaca
     3301 aaatcatcaa gggcgtaacc aagaataata aacgctttgt cacttacatg ccgatgcaag
     3361 acaatgcttt gttaggcgaa ttgttgaaaa gcaaagtcga tgtcagtggt caggaaaaac
     3421 aacaggaaag tttccttttg catttattta ttaactggtt cccaatgttg ttattgatag
     3481 gtgtatggat atttttcatg cgccaaatgc aaggcggtgg tggccgcggc gcaatgtctt
     3541 tcggcagatc aagagctcgg ctattaggtg aagatcaggt taaagtcacc ttcgcagatg
     3601 ttgcaggtgt tgatgaagcc aaagaagaag tgaaagagct tgtcgatttt ttacgtgatc
     3661 caacaaagtt tcaaaatttg ggcggacgca ttccccgcgg tgttttgctg gtcggttccc
     3721 ctggtacagg taaaacatta ttagccaggg ccgttgctgg ggaggcgaaa gtccccttct
     3781 ttaccatttc aggttctgat tttgttgaga tgtttgttgg ggtcggtgcg tcccgagtgc
     3841 gtgatatgtt tgagcaagcc aaaaagcatg cgccatgtat catctttata gatgaaatcg
     3901 atgcagtagg tagacacaga ggcgccggct tgggtggcgg tcatgatgaa cgcgagcaaa
     3961 cacttaacca attgcttgtt gaaatggatg gatttgaagg taatgaagga gttattgtcg
     4021 tagcggcaac gaaccgtccc gatgttttgg accccgcact tttacgtccc ggccgctttg
     4081 atcgtcaagt cgttgttcct ttgcctgata tcagaggtcg tgagcaaata ttaaaagttc
     4141 atttacaaaa ggtccctgta gacagccatg tagaagtgaa agctattgca cgcggcaccc
     4201 caggtttttc aggagcagat ttagccaatc ttgttaatga agcggcatta tttgctgcca
     4261 gggctaataa acgcaaagtg ggtatgattg aactggataa agcaaaagat aaaattatga
     4321 tgggggctga aagacgctcc atggtaatgg atgataacga gaaaaaactc actgcatatc
     4381 atgaagcagg tcatgctatc gttggtctgt cagttcctga gcatgatcca gtttataaag
     4441 tatcaataat tcctcgaggc cgagctttgg gcgtaactat gtttttacca gaacaagaca
     4501 gatatagtca cagcaaacgc cgtttggaaa gccaattatg cagtctgttt ggtggtcgaa
     4561 ttgcagagga attaattttt ggtcctgaaa gtgtgacaac gggtgcttcc aatgacatta
     4621 tgcggtctac tgagattgct cgcaaaatgg ttacaacttg gggcttatct gctttaggtc
     4681 ccctaacttt tggtgaggaa gaggaagaaa tatttttagg acgctcagtg aataaacaca
     4741 aggagatgtc cgatagaaca gcacagcaaa ttgatgacga ggttcgtgct attattgaca
     4801 ggaactatca aagagcaaaa gaaatattgg aaactaatat cgataaatta catttaatgg
     4861 cacaaagcct catcaaatac gaaacgatag acaccaatca aatccaagaa attatgtctg
     4921 gtaaagagcc tacccctccg gaggattggg ggtcaacaaa accgatggat aaggctgagt
     4981 cggttaatga tgctcctgca aagcctatta atggcgaaac aatagaaaat ccggcagaag
     5041 gacattaatt ttatcagtag ttatttatcg agagtaccct gtgaattctg aacagttcct
     5101 cggttggctt gagcgaaaga gccaacctcc ttcctcctta gtttttgaaa agccattaat
     5161 tatgggtgtg ttaaatgtta cctcggactc attttatgat ggtggcaagt atctgtctgt
     5221 tgatagagcc tgtgatcagg ccttaaaatt aattgcatgt ggagctgatc tcattgatat
     5281 tggtggggaa tcaaccaaac ctggtgctcc gccagtccct ttagatgttg aactatcaag
     5341 agttcttccc gtaatcaaac aacttcgtgc cctcgcagat gtgtgtattt ctattgatac
     5401 taataagcca gaggtgatgg aagcggcaat cgatgctggt gcgaatgtta taaatgatgt
     5461 ttatgcttta agaaaggatg gtgctctgaa tgttgctgcg cagttagatg ttcctgtttg
     5521 tttaatgcac atgaaaggag agcctcgaac tatgcaagaa aatccttctt acccagaagg
     5581 aataatgaag gaattacagc atttttttga agagcgcatc gataaatgcc agaaagccgg
     5641 gctgaataag aataaattaa ttcttgaccc tggctttggt tttggaaagc gcgttaaaga
     5701 taacctaaaa ttaatttatc atctgaacga attgcgttct tttggattgc caattctttt
     5761 gggcgtttca cgcaaaagca caattggtgc tgtattgaat aatgaagttg agcaacgttt
     5821 gattggaagt atcacgctag gaatatatgc tgcacttaaa ggcactgcaa ttatcaggac
     5881 acatgatgtg gatgaaacga atcaagcatt aactatgatt aatgcaattt atcaagctgc
     5941 taaagagatc agttaatagc tcatgaaatg atcagaagag aactgagatt agaaaaatta
     6001 aataataagg taggtaagga tgagtcaacg taaatatttt ggtacggatg gaatacgggg
     6061 tcacgttgga ttatcaaata ttaaccctga atttgtttta aaattaggct gggcagtagg
     6121 ctgcgttctt gcgaacggag cgaggaaaaa ggtagtgatt ggtaaagaca ccagagtgtc
     6181 tggctatatg ttggagtcgg ctttggaagc aggtttgtcc gcagctggag ttgatgtggc
     6241 attattagga ccaatgccta ctcctggaat agcctatcta acacaaacat tgagggctaa
     6301 tgcaggcatc gtgattagcg cttctcataa tttatttgaa gacaatggca ttaaattttt
     6361 ttctgctgat ggaggaaagc ttccggatag tgtagagctt gcaattgaag cccaattaga
     6421 aaaacagttg caaactgtac cttcagccaa gctgggtaaa gcaacacgta ttaatgatgc
     6481 agcgggtcgt tacatagagt tttgtaaatc tacgattcct tccctatctc gactatccaa
     6541 cttgaaaata gttgtggatt gcgccaatgg ggcaacctat catatcgccc cgaatgtatt
     6601 ctctgaattg ggtgctgatg tagttcctat aggaattaaa ccagatggtt ttaatatcaa
     6661 tcaagaatgt ggttcgacag cgcctgaatt attaagagaa aaggtgattg ctgttggggc
     6721 tgatattggt attggtttgg atggagacgg ggatcgagtt attttagtag attcgctggg
     6781 aaatctcgta gatggcgatc aaattattta tatcattgcc aaggacagac accaacgagg
     6841 agtactgcat ggaggggtcg taggcacgtt gatgagtaat tatggtcttg aattagcaat
     6901 tacctcattg ggggttcctt ttcaacgctc aaaagtaggt gacaggtatg ttcttgaaac
     6961 gctaagagag aaagattgga agattggagg tgaaacttct ggacatattg tttgtttgga
     7021 taaaacaaca acaggtgatg gaattgtagc agcactgcaa gttctttcaa tcatggtgaa
     7081 gcaaaataaa gcactgcatg agttaactgc aggtattcaa ttgttacctc aaactttagt
     7141 gaatttaaaa acgaataatg ctgccttgtt agcctctaat cctgatgtaa ttcaggcggt
     7201 taaaaatttg gaaaaacatc ttaatggaga gggtcgagtg ttactcagac cttctggcac
     7261 tgagccttta ctgagagtta tggtggaagg tgctaatgca tcgatagtca agcaacaagc
     7321 acaaatgtta tgtgatgaca tcagtcagat cgataaaaaa atgacggaga gcctaccgtc
     7381 cacttgagca tctgtctttt taagttctaa tgcaggggct tttccaaaag cccctctgtt
     7441 tcacagtatt aaggtattaa ctgcttgttg gagccatgga gtggagcatt gtgtcagcct
     7501 cttcaagtaa tcggtgagat cttgttttga aaaaacaagg cctttttgaa tactcatatt
     7561 catcttgccc tgttaactta cctattaaca ttcggaaaaa atcttcaata tattcccatg
     7621 cttttataaa tcctctatgc cgttttaaag gctcatatcg gggatcttga gtaatgagag
     7681 gcatggcttc tcggcttgaa gtttttgcat cagggagttc catcaaatgt ttaattgaaa
     7741 taagagaatg agttgcctga ataaataatt cagcttccaa tggatgttcc tttttcaatt
     7801 ttacatattg ctgatgaaat ttttgcaatt cttcaggcca attctttaac tgctgttctt
     7861 catcaatcga ttcatcaagt ataggcaaag caggttttaa acggagaata ttttgtatat
     7921 cttgtaatgg gtttgggcac atgcgcttgt atgccagtaa tacagataaa tcattttgat
     7981 tttttaattc aaacaagatg tctttgaaaa gatgaagtgc tttaagaccc aatgcctcat
     8041 ttaaacaaga actttgcgct gcatcatata aacctttata ccatagctgt atttcttctg
     8101 attgttgctt tatatattcc atgttttgta catgaaatga atctaatttt tgcttaagca
     8161 ggatagaaga tggtggcaaa ggggagatgt taggagcaga ttcctggtgt tgaggatttg
     8221 ataatttatc taactcatgg cctgtagaaa ccggttcttc tttaaatgct ttatcaattc
     8281 ctaaagaatt tacttttcct ctgtctatgg caagcgtctc ggaatcttct tcagatgaga
     8341 tagtaatttg attaccagcc tcatcaaaaa aggctaaaat attatcttct gataattcat
     8401 cgtgaccatt gaataatgac aagccatttg gtttcctgga tataactgga acctcagggg
     8461 ttgatggtct caatggttcc gatgggcttg atttgacaac agccagtgtt tcttgtccgt
     8521 tgggtacttg ttgttcttca ctggactcag agccttgctg aaccacttgg gggcggagtg
     8581 ttaattccgg aacagcaggt atttcttgct ctatctcttt atgctgcttt tcaccagaaa
     8641 gcccagagtc tttctgaacc ataggagtgc ttcgtgttga ttcaagaacg attggtgtat
     8701 cttgtctctc agctacttct agtttttcta cagtaactcc ggaatccttt tcgaccatag
     8761 atgagggccg tgttaattcc agtactactg gtatctcttg ctctacttct acacgctgtt
     8821 ttttaccagc agattcagac tcagtatgaa tgatttgagg agggtgtgtt gattcgagaa
     8881 caaccggtat ttcgtgtcca ttgtctatct gccctagttc gacgagagat tcaggctctt
     8941 ggtgaattac ttgatggggg cgtgatgatt ccagaacaac cggtgtttct tcttcattgt
     9001 cttgctgccg tttttctagg tgagaatcat tgcctatctg aaaaacttgg gcagggtgca
     9061 tcaattccac agtaataggg attgctttgt ctactgctat ttcctgcttt tccaatgaag
     9121 gatcaatttt ttcaccagtc agattaggta aaggcaattg atttaattca gacacaaggg
     9181 attggatttg attggcaagt gtagataaat cttctttaat agagtttatg tcttcgttct
     9241 ttagtggttg atttgaaatc aaacgcaatt gctcaataag tgcttttgct tcattggttg
     9301 tttgtccatg atttttcata agcaaatcaa tttgcttggc tgtttcaaga taagattggc
     9361 atagtttaat ctggctttgt aactcttctt tgagaggatc ctctgtattc aaggtatctt
     9421 tattttcttc agcttcttgc aaacttggaa gttgtacatc catcgttgca ggtaaatcat
     9481 atccacttct taattggtgt atttgtttgc ctagagcagg catttcttga agtttttcca
     9541 acgcttgtga atattgttcc ttcttttccg aaactttggg catgagaatg agaagtacca
     9601 aataaaaatc tgcaggtgaa gagtcataag aaaggccact tggtaaaaca aaatttggta
     9661 tactttgtat tggtatttca tttatcagat caatggcatc aggaactaac ttcttataaa
     9721 tagatatatc ctcttcattt gaacgagttt ctggtatcgc tttaatcaac aattcttgct
     9781 gtttcacaag ctcatgatgt ttgattttta gctctttttg ttgagctaat aattgcagga
     9841 agctaactga gtccttaagt ttttgctgct tatcagaata acttgagtcc gaataccatg
     9901 aaagccagga agatgggtta aaattagata atattccatc aatctgattt tggtattcct
     9961 gtatcaatcc ttgagggtct tcactttctt gaaatttcct gattaactct gcttttaaat
    10021 cgggtaccag ggagtggtta gtcactgtag cttcaatacg acccaggtct ttaagcaata
    10081 gttcaatttg tttttctagc tcagtctctt ttttattaat atcagttgca gataattgct
    10141 tggcgaatcg cttttgcaat tcatcccatt tttcgcaaga gcgagttaat tttttaattt
    10201 ccagttcaac tgcagctgta aatttttgta aactatcaac agcatcttct tccacaggtg
    10261 gttgaatgcc caactgagac aaatctgtca ttaaggcatg ccatttattt ttgagcaaaa
    10321 ccagctttaa tttggcacgc gtcgtttcta tttccttgct ggttttgaag agataactgt
    10381 agtgcccgac aacaattgat ttttgaacct ctccatattc agggaattta taaggaggtg
    10441 ctaatggatt ttgtttggac aggacttcgt tcgctttagg ttcgatttct ttgtataaag
    10501 gatttaattc actttttgcg agttcgattt ttttagccaa atcagaaatt tttctttgca
    10561 gttcttctcc ttttttcatg acctcatctt gtttttgttt tttcctttta tactcatctt
    10621 gttcttcagc taaactggaa tattcatcaa catgtttttt aattaacaat aaaagattta
    10681 attgggattc aacttcgaca ggagcttttc cttccggcag tagggttgaa ctaaatattt
    10741 gctttaaatt taaggggtta attggtggag tcaaggcaga gatttcattc attaagtgca
    10801 gtatgatttc gttcattata aaaccctcct ggttaaatca tagtaggatt ctatcgaaat
    10861 caaaccacta agcaagtaga aaaatgtgtt ttttgcaaaa aaaatgaata attaattctg
    10921 aaaaaaaaca tagataaagc tacacatctg agcgaagttt cggtatattg cacagctgca
    10981 cactatcagt tgtttactga taggctggaa ataatagaaa ggatttggtc cgcctatact
    11041 agaaattgtt ggagggagtg tgaggcaaaa gatagttgct ggtaactgga aaatgaatgg
    11101 ccagattcaa caggtgactg aattggtttc gcaaattgag gaattaattg gctttgattg
    11161 cgcagcacag gttgctgtaa tgcctcccag catttatata cccaaggtaa gagactgctt
    11221 aaagacgggg aaggttgtag taggtgcgca aaatgtttat ccaaaagatt atggtgccta
    11281 cacaggtgaa ctgtcagctc ccatgcttaa agattttgat tgccgatatg ttctggtggg
    11341 gcactcggag cgcaggcaat tttttcatga agatgaaaat tttgtggcgc aaaaattcca
    11401 ccatgtaaaa gatcatggta tgatacctat tctttgtgtt ggtgaaactc tttctgaaag
    11461 agagaatgga aaaacagagc aggttattgc tcaacaggtg ctcgcagtga gtgcaaaagg
    11521 gaaagattgt tttcgtgatt gcgtagtggc ttatgagcct gtatgggcaa ttgggacagg
    11581 aaaaactgct acacctgaac aggcacaaaa aatacaccag tttattagag atctggttgg
    11641 agaaataaat gatagcgatg cgaaacattt gacgctcata tatggtggca gtgttaatga
    11701 aaataatgca aaagccttat tttccatgcc agatattgat ggagggttag tgggtggagc
    11761 atcgttgaat gcaaaacaat ttgtggaaat tgtgaaatgt atcaactgat attaatgatt
    11821 catgtcttaa ttgctgtaat tttaataggc ttggttctta tccaacatgg taagggtgct
    11881 gatataggtg ctgcttttgg ttctggcgca tcaaacacgt tatttggaag tcaaggtact
    11941 ggtggcttct tgttcaagtt aactggtggt ctggcaatga ctttttttat taccagtttg
    12001 atgctgtctt atatggtttc tacgcagaac caaaaggcag gacagttagc tattcctcaa
    12061 caaaccggtg ctccagtcag ttcagtccca gtgccagttg aaaaaaataa taagaagtaa
    12121 ctaaaaagat gtagtgtctt tagggctgtt gtcttttgtg atgaactcag gataacaaaa
    12181 ataaaaataa gccgaagtgg tgaaattggt agacacgccg tcttgagggg gcggtggcgc
    12241 aagccgtgcc ggttcgagtc cggccttcgg caccatttta aatcgagcaa ggtgaactga
    12301 tgctatctca atatctaccc gttctcgttt ttataattgt tgccttgatt ttaggtgcag
    12361 taatgatagg cgctggttct ttgctttcca agcatagtcc gaatagcgct aaaaactccc
    12421 cctatgagtg tggttttggc gcctttgaaa gctcccatat tccgtttgat gtacggtttt
    12481 atctggtggc tattttattt attatttttg atctggaaac agcattcttt ttcccttggg
    12541 ccttggcatt acgtaaaata ggatggtttg gcttttatgc tatgatgtta tttcttggac
    12601 tgttggttat aggttttatt tatgaatgga aaagaggcgc cctggagtgg gaataaggtt
    12661 ctttcattct tttaaaatat ttgatttaat ctttcgtttt agccattact atgggaagca
    12721 aaggacgctt agcaaatcat tacagagctt tttgctttta gaggaacctg atggatttta
    12781 ggttggtttt taaatcctgg ttgtcctttg ccaaaacgat agctgagatt atattttaag
    12841 tcattgaata atggttatat aaaaactggg ttattaatta tttaactgct tagaggcgaa
    12901 ctatggcggt tgcagagcta gaaaaaaaag gttttgagtt gactacggtc gaaaaattgg
    12961 ttggttgggc acgcagtggc tctatgtggc caatgacatt tggcttagct tgctgtgccg
    13021 tggaaatgat gcacgtggga gcagcacgct atgatttgga cagatttggt attattttcc
    13081 gtcccagtcc acgccagtca gatgtcatga ttgtagcagg gactttatgc aacaaaatgg
    13141 ccccggctct cagaaaagtt tatgatcaga tgccagaacc acgctgggtt atttctatgg
    13201 ggtcttgtgc caatggtggg ggatattatc attattccta ttcagtggtt cgtggctgcg
    13261 atcgtattgt gccagtcgat gtctatgttc ctggctgtcc accaacagca gaagcgttgt
    13321 tatatggtat tattcaacta cagaataaaa tcaggcggaa acctgtttta gaggcataag
    13381 ctcatcaagg gtgcagatta gatgacaaaa aatgagtatt tgattgaaaa gctacaagct
    13441 aatttagcaa atcatatcac cgaattgacc tccgcatatg gtgaagtaac cattgagtgt
    13501 gaggtgcata atttattgcc tgttatgatt gagttgagag acagagagga attttctttc
    13561 gatcagttaa ttgatctatg tggcgtagat tatttgcatt atggtgatta tgattgggaa
    13621 acagagtctg cgacagagca tggattttca cgaggtgtcg agcgtcagga agctaaggct
    13681 tatgctgtaa ataaaccacg attcgctgta gtctatcatt tattatcaac caaaaaaaat
    13741 catcgtttac gggtcaaatt atttgtagaa gaatcccatt taattgttcc atcagtacat
    13801 catttatgga agtctgctaa ctggtttgag cgggaagctt atgatttata tgggatttta
    13861 ttcgatggcc atcctgattt aagacggtta ctgacagatt atggttttat aggccaccct
    13921 ttccgcaagg attttccact cagtggagag gtggaaatga gatatgatgc aaaactgcaa
    13981 aaggtaatat atgcccctgt agacattgtg ccaagaattg tagttccaaa ggttattcgt
    14041 aacgataacc gctacatagg taatgaaggt agcaaaaatg attgaactta aaaattatac
    14101 cttgaatttt ggcccacaac atcctgctgc acatggtgtt ttaagattgg ttcttgagtt
    14161 agagggtgaa actatcgttc gtgctgatcc acatattgga ttgcttcatc gtgcaacgga
    14221 aaaattggct gagaccaagc cttacattca aagcattggt tacatggaca gattagatta
    14281 cgtctcaatg atgtgtaacg aacatgctta tgtcatggcg attgagaagt tattgggtat
    14341 agaaccgcct ttgcgagcca agtatatacg aactatgttt gatgaggtga ctcgaattct
    14401 gaaccatctc ttgtggttag gagctactgc attggatatt ggtgcaatga cagtgtttct
    14461 ttattgtttc agagagagag aagatttgtt tgattgctac gaggcagttt caggagcacg
    14521 tatgcatgct acctactata ggccaggagg ggtagcaaga gacttgccag atactatgcc
    14581 tcaatacaaa ccgtcaagat ggcataacga aagagagatt gaaaaaaaga atcacaatcg
    14641 tcagggcagc cttttggatt ttctttgggc ctttactgag cgatttcccc gctgtgtgga
    14701 tgaatacgaa acattgttaa ccgataatcg tatatggaaa caacgtactg tagatattgg
    14761 tatcgtttca cctgaaaatg ctctgcaatg ggggtttacc ggaccaatgt taagaggctc
    14821 cggtattgcc tgggatttac gcaagaaaca aagctatgct gcttacgaca gggttgattt
    14881 tgatattccc gttggcaaga caggggattg ttatgatcga tatttggtaa gagtcgagga
    14941 attaagacaa tccaatcgaa ttataagaca atgtattgag tggttgcgaa agcaccctgg
    15001 cccagtaaaa gtcgatgatt ataaagtgac gcctccacgt cgggtagtaa tgaaacatga
    15061 catggaagca ttaattcatc actttaaatt gtttaccgaa ggtttttgtt taccgagagg
    15121 tgaggtttat tcatctgttg aagcgccaaa gggcgagttt gggatctata tggtgtcaga
    15181 tggagctaat aaaccttacc gtctgaaaat aagagcccca ggctttgctc atttatcaag
    15241 ctttgatgat atggttagag gccacatgct agctgatgga gtggcgatat tggcaagtca
    15301 agatatagtg tttggagaga tagatcgtta atttaacgac atgcttgctt aaagtgtatt
    15361 aagagataat aaacgatata aaggtttcga aatgtctgct aattcagcaa aaatattaaa
    15421 tcaattgatg ccggcgaacc gtatgcgcga tattgatcat tggatatcaa aatatcccaa
    15481 agatcaaaaa caatccgcgg tcatgagtgc tcttcgaata gtacaagaag aacatggtca
    15541 tttaacaacg gaattaatga atgcagtagc tgaatatctt gatatgccgc ccatcgcagt
    15601 ttatgaagtg gcaagttttt atacaatgta tgagcacaag ccagtggggc gacatttaat
    15661 taatgtttgt accaatattt cttgcatgct gagggattct gctggcgttg ttgaacattt
    15721 agaaaaaaaa ttgggtgtta atctaggtgg aacaacagaa gatggtcgat ttactttaag
    15781 atcagttgaa tgcttaggtg cttgtgtgaa tgcgccaatg atgcaagttg ataaagacta
    15841 tcacgaaaat ttgactgctg aatctataga taaagtattg gaacagtacc aatagaaaat
    15901 aaaggtgttt gccatggttg aactaaatca agtttgttac cgaacatttc atttaccaga
    15961 gccctggtcg ctatcaactt atgaaagtat tggtggctat agcgcatggc gaaaaatttt
    16021 aaaagagcaa acatcacctc aattaataat tgaagagttg aaaacttctg ccttgcgcgg
    16081 acgaggaggg gctggattcc caaccgggtt aaaatggagt tttatgaata gaaactcccc
    16141 tgttcaaaaa tacgtagtat gtaattctga tgaaggcgaa ccgggtactt gcaaggacag
    16201 agagattctt actttaaatc ctcaccaatt aatcgaagga atggccatag ctgggtatgt
    16261 tatgggcgcc acggttggtt acaactacat aagaggagaa ttctggctgc catttgagcg
    16321 tactgaggct gcaataaaag aagcatacga tgctggcttg ctaggtaaaa atatattagg
    16381 ctccggcgtt gattttgatt tatataatca tttaggagcg ggagcttaca tttgcggtga
    16441 agaaactgca ttgcttaact caattgaagg aaaaaaggga atgcctcgtt ttaaaccacc
    16501 attcccggca aattatggct tgtatggtaa accgacaacc ataaataaca ctgaaacatt
    16561 tgcgtctgtt ccggtaatta tggaaaaagg tggagaatgg ttcttaaaat tgggaaaacc
    16621 gaacaatggc ggtgcgaagt gctttagtgt tagcggccat gttaacaaac ctggtaacta
    16681 tgaaattccc ttaggcacgc cattcaaaac gctccttgat ctggctggtg gcgtcattaa
    16741 aggccgaaaa attaaagctg tgattcctgg tggttcctct atgccagtgc tcccaggtga
    16801 cgtcatgctg gggctggata tggattatga cagtatacag aaagctggct caggcttggg
    16861 ttcaggcgcg gtcattatca tggatgaaac cacctgcatg gtggatgctt tgtatcgtat
    16921 ttctgagttc tacatggatg aatcctgtgg acaatgtaca ccttgtcgag aaggcacagg
    16981 ctggttagtc aggttattac acagaattat aaatggtcat ggcgagccgg gagacgtgga
    17041 gaagttagtg aatgtggcga ataacataga gggaagaacc atttgtgcat taggcgaggc
    17101 ggcagcttgg ccagtacaga gttttgttaa acatttctat gatgaatttg aatatttcat
    17161 aaagcataaa cgctcgatcg tcgcagcata attaagaagg ttagaccatg gctactattg
    17221 aaatcgacgg taaaacattt gaagcagaaa acggtaaaat gattatagag gttgctgatg
    17281 aggcgggcat ctatattcca cgtttttgct atcataaaaa attatctgtg gcagctaatt
    17341 gccgtatgtg tttggtcgaa gtggaaaatg ggagaaaacc ggttcctgcc tgtgctacac
    17401 ctataaccaa tgggatgaaa gtatttacaa agtcagagca ggctatacac tcccaaaagg
    17461 tagtgatgga attcctcttg attaatcatc ctttggattg ccctatctgt gatcagggtg
    17521 gagaatgtga gcttcaagat atttctatgg ggtttggcgc tgataaatca gagtacacag
    17581 aaagcaagcg tgcggtagac gatgagaatt taggaacgct aatatctact gaaatgacac
    17641 gttgcatcca ttgtacacgc tgtgtacgct ttggcgagga aattgcgggg gttagagagc
    17701 tgggagctac cggccgtggc gaaaagacgc agattggtac ttatgtagaa cacagtatga
    17761 catcggaagt ttcagggaat atcatagact tgtgccctgt tggtgcttta acttccaagc
    17821 cttatcgttt taaagcccgg gcatgggaat tgacccaaca cgacagtgtt gcgcctcatg
    17881 attgcttggg gtcaaatgtt catattcata caagaaataa taaattattg cgtgtagttc
    17941 ccagggaaaa cgaatcgatc aacgaaacct ggctttcaga cagagacaga tttagctatt
    18001 tgggattaaa tgcaacttca agggcaagca aaccacaaat caaaaaaaat ggccagtggg
    18061 aagtagttga ttgggaaact gcgcttaagt ttgcggcaga agggataagc cgagttatca
    18121 aacaacatgg cccagagcaa atggccgctt ttgcgtccag ttcttctacc ttggaagaaa
    18181 tgtatttact acaaaaatta atgagagagc taggtgttca aaatctagat caccggttac
    18241 aacaaattga ttttagagat caggcatttt tacctacaac accagtaagc actttgcctt
    18301 acgcagagat agatcagcag cattcaattc tgttaatagg atgcaatatt catagagaag
    18361 taccattggc aggaacacgg gtaagaaagg cttttcgcaa tggcgcaaaa atttatgccc
    18421 ttaatcctgt agattttgat tatcattttg atttgtccgg acgggtcgtc atttctcctc
    18481 tggaaatgcc gatgcaatta gctaaattag ccttagctct tacatccgag ttagcatcgc
    18541 ttccagaaga agttcaaaaa ttgttaatag gtttggaagt agataaacaa accaaacaaa
    18601 tagcacaatc attaaaagaa gagaaagcct gtttgataac aggagcaata gttgaaaatc
    18661 atccagaagc ttccttattg cgtactttgg tcgctattgt tcaaaagctg agcggagcca
    18721 aattagtcag attaacgact ggagcaaaca gcgcgggcgc atgtattgca ggtatgctgc
    18781 cgcatagaac agttgctggg aaatcaattg cagagccagg tctcaatgta caagaagcgt
    18841 taaattccaa gttaaaaggt tatttactca tgggcgttga accaggctat gattttgcaa
    18901 atccagccgg ggcaaggcaa tcaatgttag ctgcagagtt tgttgtactc ttatcagcct
    18961 atgagcatga atccatgcat gattatgcag atgttatttt accgatcgcc ccttatgctg
    19021 aaacatctgg cacatacata aacatcgata atacctggca aacagtgaaa ggggccatgc
    19081 tgccacttgg agaatcccgg ccagcctgga aggtgcttcg tgttttgggt aatttgctgc
    19141 actgtaaaaa atttgattat acctcaacag aggacatctt ggaggaagtc aaagaagcag
    19201 tatcgatgac aatggagcat gaatacgaac cttattatcc tgaatcattg cctgtcatta
    19261 atcaatcact ggtaagagtt ggagaatggc ctctttatcg aatagatgct attactagaa
    19321 atgcaaaaga gttacaatta tgtgccgcat ccgaatctgc ctgtataaga attcatccat
    19381 caactgcaga tagattgaaa ttagaagaaa ttgctactgt atctcaagga gatattgaga
    19441 taacattgcc gctaaaacgt gatgagcgca ttgcggttga tgttgtgtgg gtggcaaatg
    19501 caatgccaga aacagttgac ctcggtcact cgtttgctgc aataacaata aagcgctaga
    19561 aaggtaaaaa aatgcttcat actattggca tactaatttg gattattatt aaaattcttg
    19621 tgatagttgt ccctttactg ctttccgtgg cctacctaac ctatgcggag cgtaaagtga
    19681 ttggctatat tcaagtgcgt atcggcccga acagggttgg tttaaagggc ttgctgcagc
    19741 catttgctga tttactgaaa ttaattacta aagaaatcat agttcccact cgatcaaata
    19801 aatacctttt tgttattgct ccactttttg ctttagtccc ttccttagtc ggctgggcgg
    19861 taatcccttt tcaggaaggt gttgttctgg caaatattaa cgcaggagtc ctatacttat
    19921 ttgccatgag ttctttgggt gtatatggtg ttcttattgc agggtgggca tcgaattcca
    19981 aatatgccat gtttggggcg ctgcgtagta ctgcccaaac agtctcttac gaaattgcaa
    20041 tgggctttgc tcttgtcggg gtactcctgg ctgcaggaag catgaacttg actgatatcg
    20101 ttaactctca aaaaggcgga atgctgcatt ggtggtttat tcccttactt cccctattct
    20161 tggttttctg gatcgccggt attgcagaaa ctaacagagc tccttttgac ttggcagaag
    20221 gtgaatcaga aattgttgct ggtttccatg tggaatattc tggtataggg ttcgccttat
    20281 tctttctctc tgaatacgcc agtatgattt tgatttcaac atttatggct attttattca
    20341 tgggcggatg gctgtcacct tttgaaggca ttacgttcct ggatcaaatc ttttttgtag
    20401 tacccggatt tgtttggtta cttctgaaaa ttagtttctt cctttttgtg tatttgtggg
    20461 taagggctac tttcccccga tatcgttatg accagcttat gcgtttaggc tggaaggtac
    20521 ttatccctgt gactatagtt tggttagtag taacctccct catggtggtt gctcatgtca
    20581 agccatggtt ttgacggtta gaagagaagc cgatgaaaaa agtatatcat tatataattc
    20641 actatgttcg tacctactta ctgttagagc tattagccgg tttatggcta acagtaaagt
    20701 attttttcag gaaaaaaata acagttcagt ttcctgaaga acaaacgcca ctttctccaa
    20761 gattcagggg acttttggca ttacgccgtt atcccaatgg tgaggagcgc tgcattgcat
    20821 gtaaattatg tgaagcagtt tgtcctgcat tggctattac aattgaatca gagcaaagag
    20881 aagatggatc acgccgtact actcggtatg atattgatat gtttaaatgt attaattgtg
    20941 gattatgtga agaatcttgc cctgttgatt cgatcgttgt cactccaatt catcactacc
    21001 atatcagcga acgtggtcaa aatattatga caaaagagaa gcttttagca gtaggcgatt
    21061 tgatggaaac acaactcgca gcagacagag ctgctgatga aaaataccgt tgatggggtg
    21121 atttatgcat gaatgggtaa tccaagcaat tttttacatc tttgcattat tgaccgtatt
    21181 gtcggcacta atggtaataa gccaaaacaa tccagttcgt tgtgttttgt ttttagtgct
    21241 cacttttttt accagtgcgg ttttatgggt tttagctgag gctgaatttt tagcattaat
    21301 ccttatcctg gtatatgttg gcgctgtcat gactttattt ctgtttgtcg tcatgatgtt
    21361 aaacatagac atcgaaagca tgaaaagtca tttaattaaa tatctaccat ttggattaat
    21421 tatagttgcc ttgttgaccg ggttattaat ggtagctatc cctaaagatt tatttaagaa
    21481 cagtgtgcaa attcaagaaa agcctttgag tgttagctca caattaatgg atttatctac
    21541 tcaagatgag ctggttccac ctgaaaacgc tacatcgaat acagaaaagt taggaatggt
    21601 tctttataca gattatattc ttgcctttga aatagctgca gtcattttgc tggtagctat
    21661 tgtgtctgca attaccttgg tgcatagggg cgctatcagg tcaaaacgac aagatatcac
    21721 tcagcaaatt atgactcgaa gggcagaacg agtgaagttg ataagtatga agtcagagaa
    21781 atagttattg gggaattctt tatgatacct gtttatgatt atttagtatt aggagtgatt
    21841 ttattcggct tgagcctggt tggtattatg ctcaatcgta aaaatatcat cttattactt
    21901 gtttgtgttg agttgatgct cttggctgtg aataccaatt ttattgcatt ttctcattac
    21961 tacaatgaag taggcggaca ggtatttgtc tttttcattt taactgtagc tgccgctgaa
    22021 gcagcaatag gtttggccat agtcatgttg ctttatagaa acagaggcaa tattgacgtt
    22081 gataaaatga atcatttaaa aggttaaaat cgtgagtatt caacaagtct gcttagtaat
    22141 cgttttgact ccccttgttg gttctgctat agcaggattt tttagaaacc agattggacg
    22201 agtcggagct catactgtaa ctattttagg cgtagctatt tccttagtat gttcaatttt
    22261 tcttgcgtgg ggcctatttt ctggttcgat cccaaacaca aatagccttg tttatcactg
    22321 ggctagcggg ggggctttaa ttccctacga atttaatatt ggctttttaa tagacccgct
    22381 aagcgttgtc atgctggtta tagttaattt tgtttcttta ctagtccatg tatacagtat
    22441 tgggtatatg gcagatgatg atggttatca acgatttttt agttatatct ctttatttac
    22501 attcatgatg ctcatgttag ttagcgcaaa caactttctg caattatttt ttggctggga
    22561 aggggttggg ctcgtttctt atttattaat cggattttgg tatcaaaagg aatctgcaat
    22621 agaaggcagc ctgaaagcat ttcttgtaaa ccgtgtagga gattttggat ttgtactagg
    22681 aataggttta atttttgcct atgctggaag tcttgattat gaaaaagtat ttaccagcgc
    22741 aaattatttg gcaagccaga atatagagtt atttagcggc tactcctggt cactgattac
    22801 agttatctgc ctgctgcttt ttgttggcgc catgggcaaa tcagcgcaag taccacttca
    22861 cgtttggtta cctgagtcaa tggagggtcc aacccctatt tcagcactaa tccatgcggc
    22921 gacaatggtg acagcaggtg ttttcatgat tgctcgtatc tcacctttaa ttgaattgtc
    22981 tacagctgcg cttagcacag tattagtgat cggggctaca ggagcattgt ttacaggaat
    23041 actggctttg gttatgaacg acatcaaacg cgtggtggcc tattctactt tatcacaact
    23101 aggctatatg atggtggcaa tgggtgcctc agcttatagt gccggcatgt ttcatttatt
    23161 gacacatgcg tgttttaaag ccctattgtt tcttggtgct ggttcagtca ttattggcat
    23221 gcaccatgaa caagatatgc ggaaaatggg cgggttatgg aataaaatgc cgattaccta
    23281 tgtaacctat gtcataggca gtttggcatt gtgtgcattt cctccttttg ctggctttta
    23341 ttcaaaagat accattattg aagcagcgca gctttcccag atacccggca gcagttatgc
    23401 ttatttttgt gtaacagcag gcgctatggt tacagcatta tatactttca gatccttgtt
    23461 catgacattt catggcaagc ctcgtatgga tgaacataca ttaagtcatc ttcatgaatc
    23521 tccatgggta gtatggttac cactagtatt gctggctatc ccatcaataa ttttaggcta
    23581 tgtgctttac atgcctattc tatttgatac accaaccctc ttaggctcat caatttttgt
    23641 tttacctgag cataatgttt tagcagaact tgctcatgaa gtaagttcgc catttgcaag
    23701 tgctgttcac gcgatttatt ccttgccatt ttggattaca gtactcggag cagttattgc
    23761 ctgggtttgt tatattgcgg ttccatctat tcccggttat ttagccaggt atttttcaat
    23821 tatttactct attttactta ataaatacgg gtttgatcgc tttaacgagc tgttctttgt
    23881 gaaaggagct agaggattag gcattgcttt ttacaagatt ggtgatcaga aactaatcga
    23941 tggtgcagtt gtcaatggta gtggacgcct ggttcgctgg ttctctggga aagggcgaaa
    24001 gatacaaagt gggtacatct atcattatgc gacagtaatg gtgtttggat tactggcctt
    24061 tttatgctgg ttaatcctgg actgaagata aggtgaaggt atgcattatt tgctcaattt
    24121 attaatttgg ttaccaatat taggtggcgt ttttgttttg ctgactgggg atgataaaaa
    24181 tccaaatgta tcgcgttatt taacattatt caccatactc ctctgtttag tgctctgcat
    24241 ccctttagtt aaaggatttg atctgcaatc ttttggaatg cagtttatag aggaaatccc
    24301 ctggatgccg gcactgggta ttaattatac tcttggtatt gatgggttat ctttactatt
    24361 aattgtttta tctattttta ctaatttaat agtgattttg gctacttggg atagcattag
    24421 taaaaaagtt tcccaatatt tatcagcgtt tttaattatg caagggcttc ttgttggtat
    24481 attttctgcc atggatgcca tagtatttta tattttctgg gaagcaacct taattccaat
    24541 gtatctgatt ataggtattt ggggttctga taacagagtt tatgcagcaa taaaattttt
    24601 cctatatact tttttaggat cagttctcat gctggccagt ttcctgtaca tgggctatat
    24661 tgcaggctca tttaagatcg aaacgtttta tgctatcaaa ttaggcatga ctgcacaaac
    24721 attgattttt attgcctttt tcttaggttt tgcaataaaa ataccgatgt ttccagtcca
    24781 tacctggtta ccagatgcgc atactgaagc ccctgctggt ggttcagtcg ttttagcagc
    24841 aattttgcta aaactgggag cttatggatt tatccgtttt tctttaccaa tagtccctga
    24901 tgcttgcagc aaatatgctc ctttgatggt tgccttatct ttgttcgcag tagtttatat
    24961 aggactcatt gctattattc agaaggatat gaaaaaattg attgcatatt cttcaatttc
    25021 acatatgggt tttgtaactc taggttgttt cgctatattt gcaattgctc gtcatggggg
    25081 actaagcaat gccggtttaa tccttgaggg tgcaattgtc gttatgatat cgcatgcctt
    25141 tgtctcaagt gcaatgtttg caggtgtagg ttttatatat gatcgtatgc atacaagaca
    25201 gctcgcggat tttggcggga tagtaaatac catgcctata tttgcttctt tttttatgct
    25261 ttttgctatg gcgaatgccg gcttgccagg aacatcggga tttgttggtg agtttatggt
    25321 gatattaggt agcataaagg ctggtttttg ggttgcattt tgggctgcaa ctacattgat
    25381 tattggagca gcatatacgc tatggatgta caaaagagtt atctttggtc ctgtagctaa
    25441 taaaaaggtt gctgccttaa aagatatatc aggttttgaa ttaagtgctt atgtattgct
    25501 tgcaataatg gtcattgctc taggagtgta tcccaaaccc atgttagagt ttgtgcatca
    25561 aactgtaagt catactttgg cattggctga taaatctaaa ctctaacaaa gaactgcgat
    25621 aggcttaaat tgcaatcaag ttccttagca aggttaaata tgatgacatt actagaaaat
    25681 atccatgtag caataccaga aatgattatt ttaataaccg cgtgtttggc gttgctttct
    25741 gatttgttct ttcgtcataa attaaaatca attgcatttt atatatcttg tgtaggaatt
    25801 atagtttctg cagcagtaag ctttgtattt ataggaagct acaagttact tatattcaac
    25861 aaattattta taagtgatga tataagtcat ttaatgaaac tgtttattga tatcacggtt
    25921 ttgttaagtt ttgtctattc ccaaaactat ctggatgaac ggcaaatgcc tacaggtgat
    25981 tattatgttc tggggctgtt ttcaacacta ggcatgatga ttcttgtttc agcacactcg
    26041 ctgttgtccc tttatctagg tttggagtta atgtcattgc cgctctatgc aatgaccgct
    26101 atccgccgaa ctgatagtga tgcatctgaa gcggcaatga aatattttgt tatgggtgct
    26161 attgcttctg gtatgctgtt gtatggaata tccctggtgt atggggcaac aggaaaactt
    26221 gatttattag atgttgctaa tgcggtggct gtcaattggc agcaacaaaa tactcttttt
    26281 gcgtttgcaa tggtatttat acttgccggc gtaggattca aactggctgc agtccctttc
    26341 catatgtggg ctccagacgt atatcaaggg gcaccaagct ctgtaacact ttttattagt
    26401 acagcaccca agattgctgc actggggatg gcactgcgat ttttgactat tggattagtc
    26461 gacatcacca tgcaatggca gcaaattatt ttagtcatgg ctctcctttc taccggcata
    26521 ggtaatttgt tagcagtgat tcaaactaga atcaaacgct tgttagctta ttctgcaatt
    26581 tctcatatag gatatgcctt atttggagtt ttagccgcca gctctgctgg ttatgccgca
    26641 gcactgtatt atattttagt ctatgctgta atgtcagcgg ctggttttgg tttagttgta
    26701 ttgatgtcaa atcatggaat ggaaattgat agtattgatg acttaaaagg cttgaataaa
    26761 agaaacccgt ggctggcgtt tatgatgcta ttagtcatgt tctcaatggc tggcgtgccg
    26821 ccaacagttg gcttctttac aaaattattg gtattgaagg cattagtaga tgttcatatg
    26881 acttggttag ctgttgtagg tttgctattt acggttatag gtgcctatta ttatttgcga
    26941 attgttaaaa ttatgtattt tgatcaacct gaaaatgaag atatagtaag aattggtaca
    27001 ggcaatacca tagtttattc acttaattgc ctctccttac tatatttagg tattttccca
    27061 ggcgctttaa ttgcagcctg tattaatgca tttgcaaatt aattaataaa ctttaacaaa
    27121 agtacttgct tagggaccca agtatcagta tacttgccct cagttgacgc ggggtggagc
    27181 agcctggtag ctcgtcgggc tcataacccg aaggtcgtag gttcaaatcc tgcccccgct
    27241 accaatttta taaaacccca tttttggggt tttttattat gttgactagg atactgtcta
    27301 aatctaagtc agttaccagg cttgggcgag tgcgcccttt ttttgtttat acatactatg
    27361 ataaatgatg acttgatagt gctattagag cctataatca aaaatatggg ttatgaactt
    27421 tggggctgtg aatatctttc ccaagggaaa cactcgttat taagaattta tatagacaaa
    27481 ccagatggaa taggaatcga tgactgtcag gaagtgagta agcaagtcag tgctatgctt
    27541 gatgttgagg accctattcc agggcattat agtttggaaa tatcatctcc tgggataccc
    27601 cgacccttgt ttagtatctg gcaatatcag cgttaccttg gttatgaaat tcatgtaaaa
    27661 acttttaagc cagtcaatgg gaagcgaaaa ttatcaggta ttattgtttc agcatcagaa
    27721 gatacaatag tattggatat taataatgaa catcaagaaa tacttttatc aaatattgtg
    27781 aaagcaaatt tgacagtcta gagaggcgaa caatgagcaa agaattgtta ttagttgctg
    27841 aggcattatc aaacgaaaga ggcgttagta aagacgtcat tatacttgcc attcaggctg
    27901 cactggaatc tgcaactcga aagattcttg gtttagatat aggtgtgcgt atcaaaatag
    27961 attctaaaac cggtgactat gaaaccttta gatactggga tgtagtgaat gaagaagaat
    28021 tagagtttcc ggaccgccaa ctgtccttgg aacaagcaaa agagcgaaaa ccctcggctg
    28081 ctgttggtga ccgaattgaa gaaattattc cttccataga atttggaaga attgaagcgc
    28141 aaacagcgcg acaagttatt atgcaaaaag tcagagaagc ggaacgtgaa ttgatcatag
    28201 accaattccg tagcaagctt gggcagctaa tctatggaac tgttaaaaaa gtaactcgtg
    28261 acaatgtaat tattgattta ggtggtaaag cagaggcttt cttgccacgc tctgaaatgt
    28321 tgcctcatga aatgtttcgt cccaatgaca gagttcgagc atatctgtat gaaatcacgc
    28381 cgcaggcgag aggaccacaa ttgttcgtaa gcagaactcg aaatgaaatg ctcattgaat
    28441 tattccgtat tgaagttccg gaaattggtg aaaatataat agatatcaaa gctgcagcgc
    28501 gcgatccggg aaatagagcc aaaattgctg ttaaaaccaa tgatgggcgt attgacccca
    28561 tcggtgcttg tgttggtatg cgtggagcaa gggtacaagc ggtttccagt gaattaggcg
    28621 gtgagcgagt tgatatcatt ctatgggatg ataaccctgc ccaattagtc ataaatgcta
    28681 tggccccagc tgatattaca tctatcgttg tggatgaaga tactcatact atggatttag
    28741 ctgttgcgaa agaacaatta tcgcaggcaa ttggtcgaaa cggacaaaat gttcgtttgg
    28801 ctagccaatt aactggttgg acattgaatg tcatgacaac ggaagagttt gaaagcaaaa
    28861 atcaagagga atcaagtaaa atagttaatc tgtttaccaa cgcgctagaa atcgatgagg
    28921 aaatagcgac attattggta gctcatggat tttcttctct cgaagaagtg gcttatgttc
    28981 cgaaggagga gctgctggct attgaagaat ttgatgaaga gatagttgaa gagttaagaa
    29041 acagagcaaa tgacaagtta ttggagatgg cactgtcatc gggcaaagga ttatctggta
    29101 ctccagatga ttcccttatc aatatggaag gcatgacaga ggatttggca aataaattag
    29161 caagtaaagg aattacaaca atggaagatc ttgcagagca atctgtagat gaattaatgg
    29221 aaatagaagg cattagtgaa gaaaaagcag gtgctttaat tatgaaagca agagagccat
    29281 ggtttcaata aactatggtt atcgaaattg accaatacga tgctagatgc attttaaata
    29341 tattcagctt tataaaaagt tgctgagagg ggattaaata tggcggatgt gacggttaaa
    29401 caattagctc aagtcgttgg tatcccggtt gagcgcttat tgaaccagtt acaggaagca
    29461 gggttatctt ttactgatga tcaacagact gtgaatgaag aacaaaagag gatcttgctt
    29521 aatcatttaa aaggtagctc taatcgtgat attagtgctg cgcctgaaag aataacttta
    29581 agacgtaaaa gcatgtctca agtcactgtt gggcatgata tgcatagtgg taaaacagtt
    29641 aatattgagg ttagaaagaa aaaaactttc atcaagagaa gtgcaattcc tgagcaagca
    29701 gagatagaag aacccgtagt tccgcctgtt gttgaagaac ccgtccatga agaaatcact
    29761 gtggttagtg aagtgtccgc agaaactccc gaattgaaag agaccgagga gcatccggtt
    29821 attgaacctg tagcagagct tgatgaaaca gtaaaagaag aagaaaaaat cagtttagag
    29881 gaaaacacag cagagagtca ggatgagtta acccatgcaa acactgatgt aatagaaaat
    29941 cttgtggatg ttgtcgagga ggccatccct gcttccaaga aggaagaagt taaaccagag
    30001 aaagtaagta aaaagaaaca tctggagcaa actgattctg atatttctga atttaaaaag
    30061 ggtaagaaaa agcctaaata tcatactttt gaacatgatg aggaggaaca agaacttcat
    30121 cgtcgtggtg gtcgaagtaa atttaaaaag aaaaaaggaa ctgaaaaatc ggataaatac
    30181 agagaagctg aagaaacatt aacgcatggt tttgcgttac ctactgctcc aatagtcaga
    30241 gaagtattga tacccgagac aataacagtc gccgaactgg caaaacggat gtcagtaaaa
    30301 gctgctgaag tgattaaagt aatgatgtct ttaggagcga tggcaacaat taatcaggtc
    30361 attgatcaag aaacgtctgt aatcgttgta gaggaaatgg gacataaacc ggtaattatc
    30421 aaagaggatg ctgttgaaac cggtttaggc gaggcaattt ccaaaggcac aaaaacagaa
    30481 ggaagagccc cggttgttac gattatggga catgttgacc atggtaaaac ctctttactg
    30541 gattacattc gtcgaaccaa ggttgcagca ggtgaagcag ggggcattac acaacacata
    30601 ggtgcttacc atgtcagtac gccaaaaggt aacattactt ttttagatac gcctggacat
    30661 gccgcattta ccgcgatgag agctcgtggt gcccaagcaa ctgatatagt cattctgatc
    30721 gtggcagctg atgatggcgt taagccccag acaattgaag cgatacagca tgctaaggcc
    30781 gctaaggttc caatcattgt tgcaattaat aaaatggata aacccgacgc tgatcctgag
    30841 cgagttatga atgaactatc cgttcaagaa gttattcctg aggcatgggg tggagatacc
    30901 atgtttgtta atatttcggc caaatcgggt atgggtatag atgatttgct ggatgcaatt
    30961 ttattgcaat cagaagtatt agagttaaaa gcagtgactg atggtgctgc aaaaggagtt
    31021 gtgattgaat cacgtctgga taaagggcgg ggtcccgttg caacagtttt agttcaaagc
    31081 ggaaccttgc ataaaggcga tattttactt gctggctttc agtatggtcg tgtgcgtgct
    31141 ttggttagcg ataatggaga tttggttgat agcgctggcc catccattcc agttgaagta
    31201 cttgggttat cggctattcc tcatgcagga gatgaagcgg tcgttgtccc cgatgagaaa
    31261 aaggccagag aagttgcttt gtttagacag ggcagattcc gtgatgtgaa attggccaga
    31321 cgtcaaaaaa cctcaataga aggtattatg gaaaatatga cggctaccga atccaaagtc
    31381 ttgaacattg tcttgaaagc ggacgttcaa ggatcactgg aagcaatttc tgatgcattg
    31441 acaaaattat caacagatga agttaaggtc gaggtgatat ctagtggtgt aggaggaata
    31501 acagagtctg atgtccattt ggctatcgct tccaatgcca ttttaatcgg atttaatgta
    31561 agagcggatg gcacagcaaa gcgtcttgct gaacaagaat ctgtatcgat tcattattat
    31621 agtgttattt atgatatagt tgaccagata aagggtgctt tgacaggcat gcttgcgcca
    31681 caatttaaag aagaaattgt tggtattgct gaagttcgag acgtattcaa gtcgccgaaa
    31741 attggtgcta ttgctggatg catggtcatt gaaggcgttg tcaagcgtaa taatccaata
    31801 cgtgtattgc gttcaaatgt agtgatttat gaaggtactt tagagtcatt aaggcgattt
    31861 aaagatgacg tattagaagt aagacaaggt tttgaatgcg gtatcggcgt aaaaaactac
    31921 aacgatgtga agcctgggga tttaatcgag gtttttgaga cagtcgaaat taagcgtgac
    31981 ttatagaaaa tgagtaataa ttttaagcga actgacagaa ttgctgagat gattcagcgc
    32041 aaattagctt tgatcattcc ccaggaaata aaagatccgc gcttgaaggg ttttgttacc
    32101 atttctgcag ttaaagtggc tgcggattta ggtcacgcca aaatttattt tactgttctt
    32161 aatgaagata aatcggtggt gaccaatata ttaaatggtg cagccagtta tttgaggagc
    32221 gctctggcaa gaagtattac tctacgcacc gtgccgcaac ttcattttat ttatgatgaa
    32281 tcgattgagt atggacagcg gttaagccga ttaattgatg aagtaaatcc ccctgattca
    32341 agtagtgatg acaacaattg aatcgcagtg ttccatagat ggaatcctgc tattaaataa
    32401 accacaagga atgacctcta acgccgctct tcagaaagcc aaacatttgt ttggtgctaa
    32461 aaaagctggg cacacaggca gcttggatcc tttagcaacc ggcatgctac ctctatgctt
    32521 cggagaagcg actaaaatat gtcagtatct ccttaatgcg gataaaagct atgaaacaat
    32581 tgggaggcta ggcagtaaga caaatactgc tgattgtact ggcgaagtga tattttgcat
    32641 tgaaaactat actgttagcc atgaggaaat gattgcaacg cttgaaaaat ataagggtaa
    32701 aatcaagcaa atcccatcca tgttttctgc tttaaagcac aagggaaccc ctttgtatcg
    32761 acttgccaga gaagggatag agatagaacg taaggccagg gatatagtaa tcagccaatt
    32821 gaagttggaa caatttgatg gtgaatgttt tacattaacg gtatcatgca gcaaaggaac
    32881 ctatatacgt aatttagtgg aagatattgg tgatacgttg aaggcaggag ctcatatgac
    32941 aaaattgcat aggctctaca ctgcagggtt tgaaaataat cgaatgtaca ctcttgatga
    33001 attacaggat atgcctttat cacaaaggct tgcttgctta atccctattg atcaggccat
    33061 tcagcattta acaccagtaa ttctttcaga ttctgaggta acagcaattc gccagggcaa
    33121 ggtgatttca aataaaactg gtgctgttga aggagaagat ttgcgtcttt atggcgagca
    33181 atcccaattt attggcattg gccaagcttt aattcatggt gatattaaag caaaaaggtt
    33241 agtttctttt gcgttgtaat gacaattttt gtaagaattg ctgcctactt tgtaataagc
    33301 atgaaatttt ttaaacaaat ttatatccac tacttgtttc atttgacatg ccttggtaga
    33361 atgttcgcct ttaaaagaat acttagctga gttccaggag taaataatgt cgctaaatag
    33421 cgcggaaaaa gcagaaatta ttaatgaata caaacgcggt gataaagata ctggttcacc
    33481 tgaagtgcaa gtttctttaa ttaccggtcg cattaaatat ttaactgacc attttaaaga
    33541 aaataaaaaa gatttccatt cgagacgtgg cttgcaagaa ttggttaaca agcgccgcaa
    33601 gttactaaaa tatttgaagc gtaatgatca agctcgctat caaacattaa tccaaaatct
    33661 gggattgaga gattcctatt gattgatggg attattgtct ctatggggac gcatcaaata
    33721 ataaatcagt tttagaataa aaggcgcagc gttctgcgcc ttttttttct tatatttact
    33781 taatggggaa cattacgtgg caaaaattac aaaagaaata gtattcggta accataaatt
    33841 aatcttggaa acaggtgaag tagcacgaca ggctgatggt gccgtaatgg caagtatgaa
    33901 tggtacacaa gtgctagtga ctgtagtttg gaaaaaggat agtggtgaaa gtaatgattt
    33961 tttcccatta acagtgaatt atcaagaaaa attctatgcc ataggtaaaa ttcctggtgg
    34021 ctttaacaaa cgcgaaggac gaccatctga taatgaaaca ttaatttcgc ggctaataga
    34081 tagaccgatc cggccattat tcccggataa tttttttaac gaagtacaga taattgctac
    34141 tgttctgtcc ttaaatcccg aggtatctcc tgatattatt gctatgattg gagcctccgc
    34201 tgcactttca atttctggtg ttccatttaa tggccctata ggcgctgctc gagtaggcta
    34261 taaagatggc gtttatctgc tcaatccaag tagaaaagaa caagaagaat ccaagcttga
    34321 tttggttatt gcgggcacaa aagatgccat tttgatggtg gaatcagaag cgcaggaatt
    34381 aagtgaagac atcatgcgtg gtgctatgtt atatggccat gaaatgatga aaagtgttat
    34441 aaaatcaata gaagaacttg ccagagaagt gggtaagagc aaacctgagt ggaaagctcc
    34501 tgaaattgat acagtactaa aagctagaat caatgacgta gcaaggaatg aagttgaagc
    34561 cgcttatctt attaaagaca aacaacaacg ctaccagcga ttagatgagt taagagagca
    34621 aactatttct gccctgctgg ctgaaaatga tgaattaaat gcagatgtta ttgccaatat
    34681 gtttggtgag ttagaacgtt ctatagtacg taatcgtatt cttgacggcg aacctcgcat
    34741 cgatggtcgt gatcacagaa cagttaggcc tatttctatt cgtaccaaat tcttggaaag
    34801 aactcacggt tcctgtttgt ttaccagagg agaaacccaa gctattgttg ttgccacttt
    34861 gggtaatgaa cgtgatgcac agatactaga cggaattagt ggcgaaacca gagatcgatt
    34921 tatgcttcat tataatttcc ctccttattc tgtcggtgaa acagggcaag taggtagtcc
    34981 taaacggcgt gaaattgggc atggccgttt agccaagcgt gctttaatgg ctgtactgcc
    35041 tgatactaat gagtttcctt atgtgctacg tattgtatct gagattactg aatccaatgg
    35101 ctccagttcc atggctactg tttgtggaac aagcctggca ttgatggatg ccggggtacc
    35161 tttaaaagca ccagtcgctg gtgtggccat gggcttaatc aaggaaggtg atcgttatgc
    35221 tgtattgaca gacatattgg gtgatgagga tcatttaggt gatatggatt ttaaagtagc
    35281 tggtacagaa aaagggatta cagctttgca gatggatatc aagatttccg gtattaccaa
    35341 tgaaattatg gagcgggctc tggagcaagc attggaaggt cgtacccata ttcttggtgt
    35401 catgaacaat gcccttgcag agcatagaac cgagttatct cagcacgctc caagaatcac
    35461 aaccatgaaa gttgctgaag ataaaatccg cacaattatt ggcaaaggtg gggcaacaat
    35521 taaagggctt attgaaagta ctggcgtatc tatagatatt gatgactctg gggttatcca
    35581 attattttct ccggataaga tggctctgga agaggcgcaa aaacaaatta aagctttaat
    35641 tgctgaaatt gaagtgggtc agacttatca agggaaagta agcaagatag ttgactttgg
    35701 agcttttatc aatttactgc ctggtaaaga tggcttactg catatttcac aaatatgtgc
    35761 cgaaagaaca caaaaggtag aagaggtatt gcaggaaggt caggaaattg aggtctttgt
    35821 tgctgggata gataagcaag ggcgagtgaa actggagtgg aaggataagc cgcaagctga
    35881 agccaaagag gtcgaaggcg cctctgtatc tgctacgttt cttacaatgg aagagcaatc
    35941 tgaagaaatt aattcaggca acaaaatctc tgaagaagag gaataataag atagtagcat
    36001 ttcaagtttt attcattata aactcgtgga aggcaagagc ttttcgcgag ttataactga
    36061 gtgtttgcaa ttggtgtata tgaccgcata aattataagc tactaaagta gagtttaagc
    36121 atgaagctat tctctttttt tcttgtattg ataagctttg ctagcttagg tcaggcttta
    36181 ccattacatc tcctaaagta cccgcccgga ttttcagtaa gtatttatgc ggcccccgtt
    36241 tctgatgctc gagaaatggc actgggcagt aaagacatag tttttgtagg ttcaagaagt
    36301 gccgggaaag tgtatgctgt tattccagat aaaaaaaact cccatggtac tcgagtttta
    36361 accatagctt ctggattgaa tatgccaaat ggtgttgcct ttcataaagg cgcactttat
    36421 gtcgccgaga ttgggcgcat tttacgcttt gataatattg aatcaaatct gaataatcct
    36481 cctgaaccag ttgtggttac tacaaaatta ccgaaggaaa aagctcatgg ctggcgtttt
    36541 attagttttg gaccagatga taagctttat ataggaattg gcgccccttg caatgtttgc
    36601 ttaatggagg atacgcgcta tgccactatt atgagaatga atgccgatga gcaagatctc
    36661 gagatctatg ccaaaggaat acgtaataca gtggggtttg attgggatcc cgttcatcat
    36721 aatctttggt ttacagacaa tgggcgtgac tggctaggtg atgacttacc tccagatgag
    36781 ctcaatgtag ctccgagaaa aggcatggat tttggttttc cttattgcta tggtaaaagt
    36841 acagcagatc ctgtttatgg caaaacacat tcttgttccg agtttactcc tgcggttttt
    36901 gaattcccag cacatgttgc tcctcttggt atgtcatttt atacaggtaa attgttccct
    36961 cccaattatt ggggacaaat ttttattagt gagcacggct cctggaatcg tagtcataag
    37021 atagggtatc aagttactac agtaaaaatt aagaataatc acgtaactga ggttaaacca
    37081 tttatcagcg ggtggttaca gggggaaaaa gcatggggaa gacctgtcga tacgctcgtt
    37141 atgccagatg gatcactatt aatctctgac gattacgcta atgtaatata tcgggttagc
    37201 tatcagcagt aaatggagca attgttttat tataggcggg gataaagccc ctacctattt
    37261 cttatcagta ttaacggatt aaatttaaga attccaaacg gcaactcgaa ttgttacgca
    37321 tttcaccgag cataactgaa gttgtcatga cagaattttg tttttcaact ccacgcatca
    37381 tcatgcaaag atgttttgct tcaatcacta cggcgacacc gcgagctccg gtaatggatt
    37441 caatacaatg agcaatttcg ccagtcagcc gttcttgaat ttgtagtctc ctcgcataaa
    37501 aatcaacaat acgagcaatt ttagataagc ctattacttt accatcaggt agatatccaa
    37561 catgacattt gcccaaaaaa ggcaataagt gatgttcgca taatgagtac agttctatgt
    37621 ccttgacgat aaccatttca ctcatgtctg aatcaaacag agcaccgtta atgatttcat
    37681 caaggttctc attgtaacct ttagttaaat aacgtaaggc attagctgct cgtccaggag
    37741 tatcctttaa tccttcccta tttatatctt cgcctaattc tttaagcaaa tttgagtata
    37801 atttttccat agcaagaatt cctaacctgg cggccaagtc atttgacgcc cgccaagtaa
    37861 atgtaaatga atgtggtaaa cttcctgccc gccaccagaa ttaacattaa aaaccaatcg
    37921 ataacccgtt tcacttagtc cttcggcctg cgctaatttt tttgctctta ttagaatatt
    37981 agcaagaagt tcgctatcac catcgttcac atcattgata gttgcaatat gctttttggg
    38041 tataatcaat aaatgtgttg gtgcttgagg tctaatatcg cgaaatgcca aaatattttt
    38101 gtcttcaaaa actactgttg caggaatttc accctgtgca attttgcaaa acaaacaatt
    38161 cataggtcat ccgatgtttt agattcaaaa tgctattata catttcattt attatattgc
    38221 gaacgcttgt ctttgccttt tgatgcgatt tttcgcataa tattactttt atttattatt
    38281 aagggtgcgt aaatgagttt aatggaaatt cagagcggtc gtgatgttcc aaatgaagtg
    38341 aatgtaatca tagaaatccc tatgcacgga gaacctgtaa agtatgaagt tgataaaaaa
    38401 actggcgctt tatttgtaga tcgatttatg acgacagcaa tgttttatcc aacgaattac
    38461 ggttatatcc cgaatactct ttcggaagat ggggatcctg tggatgtgtt ggtaataact
    38521 cctgtccctt tgatcagtgg ggctgttatt tcatgtcgag ctgttggcat gttaaagatg
    38581 acagatgaat ccggagttga tgcaaagatt ttggctgttc caacaaccaa actaagtaaa
    38641 atgtatcaat ccatgcaaac ttatcaagat attcctcagc atttattgtt atctatcgaa
    38701 cattttttta agcactataa agacctggaa gaaggtaaat gggttaaggt cgagggctgg
    38761 gtaggcccag atgcagctcg tgaggaaata acatccagta ttaaccgtta taatcacaca
    38821 aaaaagtaaa acagtgactt gaactgtgcc aggtgttggg cattgctcag caccaacctt
    38881 atatctttag ctataatagc ggcatcattt cagcccctac tacaaaacag caagttctat
    38941 tgtataattt gtcattctta aaagtataag gatattcagt gcaacttcct ctttctactt
    39001 tattggtttt attgtttttc ctaatttgcc tgtctgcttt tttttcaggt tccgaaattg
    39061 gcatgatgtc tatcaataga tataaattgc gacacttggt aaaaagcaat gataagcaag
    39121 ccattcgggt taattccatg ctggctcggc ctgataagct actaagtgtg gttttaattg
    39181 ggaatacttt ggcaaatata gtggcatcaa ctctcgccac tttaatcgga cagcggtttt
    39241 atggggatgc aggggtggct atagcaactg ccttgttgac catactgatc ctggtttttg
    39301 cagaaatgac tccgaaaacc ctcgctgcta tttaccctca acaagtagct tttgcatcct
    39361 cattaccgtt gagcatttta caatggattt tttctccttt ggttcattta atcagtttga
    39421 tttccaatgg tatcttaaga ttatttaata tatcagtaaa acgcatgcaa aaggagatgc
    39481 taactggtga agagttgcga tcagtggttc atgaggcggg aggattaatg ccagtagagc
    39541 ataaaagtat gttgattagt ttattggatc tggaacaagc aactgttgaa gacatcatgg
    39601 ttcccaaagc cgatattgtt gggattgatt tggatttacc ctggatagaa cttttagatc
    39661 aattggaaac agcacaacac acaagattac caatttatca tggttctatt gatcatttgg
    39721 tagggatgat ccacgtaaga aatattttaa atttagcatt agaaaataag ctggatatgg
    39781 aaaatttgtt aaatagtgct gaagttccct attttattcc cgaggctacc ccattaaata
    39841 tacaaatcct taatttcagg aaaatgaaaa gaagaagttg ttttgttgtg gatgaatatg
    39901 gcgatattct tgggcttgta actatggaag atattcttga agaaatagta ggggagttca
    39961 caacagatat cgctgatctg agtcgtgata tcacccctca gcaagatggg tcggtcatag
    40021 ttgatgccag cataactatt cgccatttaa accgcatgat gggctggagc ctgcctttaa
    40081 tcggtccaag aaccctaagt ggcttgatta ttgagcattt agggtatatt ccaccccctg
    40141 actcttgctt ggttattgat cattatcata ttgaaatatt aagggtcagc gataatatga
    40201 taagaggcgt aaagatgatt aaggctaata aaaaaagaaa atagctctta tagattctga
    40261 ttctcaattg ctctgtcatt atcaaagata gagttcgatt ttatcttcca aactattgtg
    40321 gttgactcat aatgttctca ataggcttca agcttgccct tattttggtt tacttaaata
    40381 attattgatt gatgaagcga tacaatttat tagttttttc acaatagact acttcgatgc
    40441 atatatttaa gatttgattt tgaggggcaa gacaacatag agttttaaaa aaaaacaata
    40501 tatattattt taacgcattt gtgttaaaat aatgccttta gaatatgaaa tagagcgtat
    40561 gaagatttta tatgtacctt tctccagaaa tcagg
//
LOCUS       DAXLDT010000010        39492 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXLDT010000010 DAXLDT010000000
VERSION     DAXLDT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 39492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39492
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.010"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..842)
                     /gene="queF"
                     /locus_tag="ACF2SP_000432"
     CDS             complement(<1..842)
                     /gene="queF"
                     /locus_tag="ACF2SP_000432"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945780.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFD2358240.1"
                     /translation="MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQE
                     INLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNS
                     LNNSTFPDVETVVQTISKDLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIE
                     CSVYHVMPDFLSTSSKLVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLK
                     YLISFRNHNEFHEQCIERIFADIMRFCQPESLAVYGRYTRRGGLDINPIRSTEPCAFD
                     GQNIR"
     gene            990..1391
                     /locus_tag="ACF2SP_000433"
     CDS             990..1391
                     /locus_tag="ACF2SP_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFD2358241.1"
                     /translation="MINQQIEIFLKSPAFAVVGASSNREKYGNKVLRCYLRNGKKVYP
                     VNPHETLIESLPVIHSVNDLPDNVHSISIITPPSITEEVVKEAVKKGIQHIWMQPGAE
                     SDSAIQYGLDHKLNVISGGPCILVVMGYEEN"
     gene            complement(1456..2577)
                     /locus_tag="ACF2SP_000434"
     CDS             complement(1456..2577)
                     /locus_tag="ACF2SP_000434"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFD2358242.1"
                     /translation="MPLQPFKTSNLLTMGVELELQLISLSNFDLTAASPDILELLGRS
                     SFPGSFTPEITESMLEIATDVHEEYDQLLKQLFHIRDTLVTVGDRLNIGICGGGTHPF
                     QMWSNQRIFNKTRFIEVSELYGYLTKQFTIFGQHIHIGCEDGNQALFLLHSLNRYIPH
                     FIALSASSPFVQSKDTLYNSARLNSVFAFPLSGRAPFVLNWDEFSLGYFEKMEHTGIV
                     KSMKDFYWDLRPKPEFGTIEMRVCDSPLTVERAAALACYMQALCSYLLENKEPLPHED
                     DYLVYNYNRFQACRFGLDGTLVHPKTYEQILLREDILTTLRRLKPYANQLNSTMALEH
                     IYEITHKGSDASFLREKYAEHRTLESVVNESLKQFRSSK"
     gene            complement(2579..3766)
                     /locus_tag="ACF2SP_000435"
     CDS             complement(2579..3766)
                     /locus_tag="ACF2SP_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFD2358243.1"
                     /translation="MIDVGKIITDLAWPISLLIAWFAGELGYRWAKLPHISIYAIVGF
                     SLASTQIGLLPSIQPPSILLLANIGFGLILFESGYRINLRWLSYNPWVAASSITEATL
                     TFCLVYILVKSFGLQTSTCLLLASLSMATSPATVVRVINEQRSSGQVTERVLHLSVIN
                     CVLAVFLFKVIIGLVVFRTSGNLWEAAYASLTVTAISTIWGIIFGVIMPILLKTVKST
                     CQDNTLAFACAVILLVILTHNLKLSPVLATLTFGLVSRHGRMVFSPSQPGFGTLGDLL
                     SVLLFVFIAATIEWQRVVTGVWIGLSIITVRFVAKIIGISLFAHISGISFKKGILTGL
                     AMTPISVFVILILEQSRYIGINLVDTLAPLAMVALTLEILGPIMVQRALIKANEISAI
                     KEF"
     gene            4096..4698
                     /gene="def"
                     /locus_tag="ACF2SP_000436"
     CDS             4096..4698
                     /gene="def"
                     /locus_tag="ACF2SP_000436"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFD2358244.1"
                     /translation="MALEIITIEKKENYHMLKTRAENIQFPLNREDQDLIEAMKKKLY
                     ALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIYPMHILINPNYEPLPQARIISD
                     FEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITD
                     RLTPDCVQGTIEEMMTIRRAELPLEKRKLFDQIMAKKLKK"
     gene            complement(4734..5138)
                     /locus_tag="ACF2SP_000437"
     CDS             complement(4734..5138)
                     /locus_tag="ACF2SP_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HFD2358245.1"
                     /translation="MHKLIELQGVGPKYAEMLKRVGIESQEDLLRVCSGRRGRFRVAE
                     QTGISHKLILKWTNQADLARVNGINEEYAELLEQAGIDSIPELAHCNPEQLYSSLKEK
                     NEQCHLAQHVPGLSQVVTWVEQAKKLPRAVYH"
     gene            5325..5669
                     /locus_tag="ACF2SP_000438"
     CDS             5325..5669
                     /locus_tag="ACF2SP_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358246.1"
                     /translation="MRKHKNKMVRIFLLALGLFSSTTINAWYGSGITIGVARANVHYY
                     GDDIRVYDPWGYGYYDRNYYYNGVRNIIVPSVIVPNVVINVPVQRYYVPSCEEMEVCD
                     PYGQCWLEQFCR"
     gene            5870..6916
                     /locus_tag="ACF2SP_000439"
     CDS             5870..6916
                     /locus_tag="ACF2SP_000439"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFD2358247.1"
                     /translation="MSETIGYAAHKAGAPLKPFTFERRPVGAHDVAIDILFCGICHSD
                     LHQVNNDWAGAFYPMVPGHEIIGRVKEVGHQVRRFKAGDLVGVGCMVDSCRQCTYCHQ
                     HEEQYCKSGATFTYNSQDNHLGGVTYGGYSKHIVVTEDFVLKIDESLDPAAAAPLLCA
                     GITTYSPLKRAKVGKMSKVGIVGLGGLGHMAVKLARAMGAYVTVFTTSINKIKAGKDL
                     GAHDVVLSTDNNVMQTLANQYDFILDTVSAKHDLGQYLNLLKVDGQLTQVGLPSDPLE
                     VTMFPVILKRLSINGSLIGGIKETQEVLDFCARHGITADIELIKMHEVNSAYERLLKG
                     DVKYRFVIDMSTLD"
     gene            complement(6976..8289)
                     /locus_tag="ACF2SP_000440"
     CDS             complement(6976..8289)
                     /locus_tag="ACF2SP_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFD2358248.1"
                     /translation="MYNILLIFIAFLLVLLNAFFVSAEFGMVKLRATRIQAIKENYGL
                     RGRILEQVHQNLDAYLSACQLGITLASLGLGWIGEPAFAELLGPLFVALGISSVHIVT
                     ISSFFIAFFLISFLHIVIGELMPKSIAIRQSELISIWTAVPLYWFYWIMYPAIWLLNT
                     CSNFFLKLSGMDIKNEYEGFYSSEELKLILSGQHLQSELKEDEREILEHTLDLAELNV
                     TEVMRPEDEMIIIDINEPFETIKDKILKYRYSRYPVYDSGTKEMIGVIHVKDLFADLL
                     LQKNINTLKPYVRPVLKVSHKLPAMDLLRKFRAGMPHFALVYKHKSSLLGFVTLDNLL
                     HILIGRIKDEFHKTHEDWVKNSDGTITTKGDCSLYSLERALDCDITLSPDEEELNTLY
                     GLIITRHGKLPNEGEIIQFVEFDALIEKVQDSFITKIKVFPKIKN"
     gene            8943..10238
                     /locus_tag="ACF2SP_000441"
     CDS             8943..10238
                     /locus_tag="ACF2SP_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358249.1"
                     /translation="MNQNSFKPKNNFVSLVMWALPLSFFTFQFLLRLWPGLTMQDIMA
                     QFSIDAASFGLIAAFYYYGYAGMQIPVALLLDKFGARFVVFAFAMLCGIGTLVFIGTN
                     NFYLALSGRFLIGVGSAAGFLGVSKVVSEWFSHSQYSRMIGLSFSVGLLGAIYGGKPL
                     AHLIQNFSGQQVGLALAVFSILLGCCALLILRSPPNNTHSVTKTMNLSHCSSLFSSWI
                     IWALALANLLMVGALEGFADVWGVPYLIAKYALNKTDAAFLISFIFMGMIFGGPLLAF
                     LSQKLGNYFVIALCGTGLAFLFLILLYTATLHWWQLGTLFFAIGLLCCYQVIVFAAGS
                     TLVPYEGLGVTIAFLNCINMLGGSFFHTLIGKLMDSSWSGQMSANGLKLYEMESYQWA
                     LSLIPFCALCGSLLIVLIHIKTRAYAAPELMKKTSLTGV"
     gene            10241..10885
                     /locus_tag="ACF2SP_000442"
     CDS             10241..10885
                     /locus_tag="ACF2SP_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HFD2358250.1"
                     /translation="MEKIFWSNPYQHTLDTKIISINGNEILLEKTIAFSFSGGQESDR
                     AFINGLEVLSSRIDGNLIYYTLPDDHNLAPNDVITMTIDWPRRHRLMRLHFAAELILE
                     LVTRTSMLEKVGAHISESKARIDFKANFNISSIFSQLLDEYNAIIRQDLVIYKGYHDV
                     TNQRRFWKIDGFAEVPCGGTHVHSTSEVGFIRLKRSHPGKSIERIEITLMDDSR"
     gene            11132..12046
                     /locus_tag="ACF2SP_000443"
     CDS             11132..12046
                     /locus_tag="ACF2SP_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFD2358251.1"
                     /translation="MELPYHFSTAYVSKPEFIEALSEELGEVSNKIGSLVFSPHQKLD
                     VCFALDVWLEPEIVTFQSISEAAKILRQAGKFWYLHPVTEIRRSRLIEEQLRKCPSLM
                     SHFPVTSDIPPIGVFSLLDRNTLLFSAKRLKKWPDGQCYFIEDKVNPPNRAYLKLWEA
                     LFLLNKYPLPGETVLDLGASPGGWTYVMQSLGTQVTSVDKAELDTKIMALPRVSFLKQ
                     SAFALDPEQLTESYDWVLSDVACYPDRAYTLIKKWIASKKAKQLIFTIKLQGQTDLNT
                     IKRFQSIPNSKVINLFYNKHEVTFFYPV"
     gene            complement(12119..13072)
                     /gene="epmA"
                     /locus_tag="ACF2SP_000444"
     CDS             complement(12119..13072)
                     /gene="epmA"
                     /locus_tag="ACF2SP_000444"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFD2358252.1"
                     /translation="MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMAR
                     HGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHN
                     PEFTLLEWYQLHIDHHVLMDEVDLLLQIILKSRPMIRKTYQQAFEEACGIDPFSTSIE
                     QFNKVLAQHDLDGVLSPNEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALA
                     QVKEGLAERFEVYYQGVELANGFHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLL
                     KALEYGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS"
     gene            complement(13075..13410)
                     /locus_tag="ACF2SP_000445"
     CDS             complement(13075..13410)
                     /locus_tag="ACF2SP_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HFD2358253.1"
                     /translation="MFGFKKKQSNESIKEGVITALRGVFDPEIPVNIYDLGLIYDIAV
                     NDEGHVHIQMTLTTPGCPVAQTFPGTVEQAVNQVEGVSDCTVELVWEPPWSQDRMTEA
                     ARLELGIFY"
     gene            complement(13482..13931)
                     /gene="sufU"
                     /locus_tag="ACF2SP_000446"
     CDS             complement(13482..13931)
                     /gene="sufU"
                     /locus_tag="ACF2SP_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFD2358254.1"
                     /translation="MSMELRELYQEIIIDHNRNPRNHHAMEDATTEAKGFNPLCGDKL
                     TVYLKLQGDLIRDVSFIGCGCAISQASASLMTDALKGKSIKEAHELFHRVHRMLTQEE
                     EDSLVSMDKLTVLAGVKAFPARVKCATLAWHTLEAALNKETEVVKTE"
     gene            complement(13928..15172)
                     /locus_tag="ACF2SP_000447"
     CDS             complement(13928..15172)
                     /locus_tag="ACF2SP_000447"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HFD2358255.1"
                     /translation="MSTNPSLMTSFDVNKIRKDFPVLHQKINEYDLVYFDNAATTQKP
                     KAVIDAIAQFYEKDNSNVHRGVHALSVRATEMYEAARAKVKRFINARSPRECIFVRGT
                     TEAINLVAQSLVAPRILPDEEILITHMEHHSNIVPWQMVCKKMGCKLQVAPISLNGEV
                     ILEEFERKLNENTKMVAINYASNSLGTINPVKTMIKMAHEVGAKVLLDGAQATAHLIV
                     DVQDLDCDFYAFSGHKMYGPTGIGVLWGKEELLNSMTPYQGGGEMINSVSFEATEYAA
                     IPHKFEAGTPNIAGAIGLAAAIDYIWSLDLDAIAEYETQLLNYATKAIEAVKGYNIIG
                     TAANKVPIISFVHGKIHAHDIGTILDSEGIAIRSGHHCTMPLMDFYDVAATSRISMSF
                     YNTFKEIDYCMEALQRVKEVFA"
     gene            complement(15162..16448)
                     /gene="sufD"
                     /locus_tag="ACF2SP_000448"
     CDS             complement(15162..16448)
                     /gene="sufD"
                     /locus_tag="ACF2SP_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFD2358256.1"
                     /translation="MSDILDYYQQQAQLGVSQIPWLAQLQSKALTDLKRRGFPTRYDE
                     EWKYTLVDSLLQQHFVPPDPTVKPALKTAFDDSPVKNLFLIQNGFISQEQEWIKNLPQ
                     GVLILPLSLALIHYADLVKPHLGKIMEQEHGFHYLNTAMLQCGVFIYIPEGICLEEPI
                     VLKHIQDQTNQAMYLRHLILMEKESQATLIEEYHGEENICYFTNSITEVFLGAGAKLN
                     HYKIQKESKSAYHLGHLSVRQSAASQFSSHSLSLGGKLVRSDISVSMQEEQAQCLMNG
                     IYAPGDGQHIDHHTVVHHLVPNCRSDQDYKGILKGHSRAVFNGKVIVAKNAQHTDAKQ
                     QNKNLLLSANAEIDTKPQLEIFADDVLCSHGATVGQLDEEAIFYLATRGISRDEASHY
                     LIQAFANDNLRLIPNRDLADWMGNLLTQQLGYANEY"
     gene            complement(16445..17197)
                     /gene="sufC"
                     /locus_tag="ACF2SP_000449"
     CDS             complement(16445..17197)
                     /gene="sufC"
                     /locus_tag="ACF2SP_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFD2358257.1"
                     /translation="MLNIKELNVAVNGQSILKGINLEVKPGEVHAIMGPNGSGKSTLS
                     KVLAGHPSYEVTRGDISYLGQDLLPMSPEERARAGIFMSFQYPVEIPGVTNINFLKAS
                     VNAVRKGQGKNTLDAIEFLSFIREKCQLLDMDESFLYRSINEGFSGGEKKRNEILQMA
                     ALEPKLAILDETDSGLDIDALRIISQGVNAMRSPERSIILVTHYQRLLDYIEPDFIHV
                     LANGRIITSGDKSLALELEKKGYSWLEEVEQL"
     gene            complement(17199..18647)
                     /gene="sufB"
                     /locus_tag="ACF2SP_000450"
     CDS             complement(17199..18647)
                     /gene="sufB"
                     /locus_tag="ACF2SP_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFD2358258.1"
                     /translation="MAKSSEQLNSLLDREYQHGFITDIEVETFPPGLDEDVIRRLSAI
                     KGEPEFLLEWRLKAFRHWLTMPHPEWSSVHYPPIDYQSVSYYSAPKSKKDAPKSLDEV
                     DPELLKTYEKLGIPLREQEMLAGVAVDAVFDSVSVATTFKAKLAEKGVIFCPLSEAVH
                     QYPDLVKKYLGSVVPYRDNFYAALNSAVFSDGSFVYVPNGVRCPMELSTYFRINAAST
                     GQFERTLIIADVDSYVSYLEGCTAPMRDENQLHAAVVELIALDGAQIKYSTVQNWYPG
                     DKEGKGGIYNFVTKRGACRGKKSKISWTQIETGSAITWKYPSVILQGDDSVGEFYSVA
                     LTNNFQQADTGTKMIHLGKNTRSTIISKGISAGHAHNAYRGLVRIAPTATNARNYTQC
                     DSMLMGSQCSAHTFPYIEVKNPTAQVEHEATTSKISEEQLFYCQQRGIDTEDAVSMIV
                     NGFCKQVLKELPMEFAVEATKLLGISLEGAVG"
     gene            complement(18640..19101)
                     /locus_tag="ACF2SP_000451"
     CDS             complement(18640..19101)
                     /locus_tag="ACF2SP_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HFD2358259.1"
                     /translation="MLRVSKLADYGTVVMVYLAKHSQELCNARDIALHTHLTVPTVSK
                     ILKRLTSAGLLTSVRGVSGGYRLQRPAAEISVSQVIFALEEHRGFTECSMQPNDCFLQ
                     GVCTIQGNWRLISHAIETALDSVSLEALAKPTLPTRDVERVRQLASGVNRG"
     gene            19542..21284
                     /gene="phaC"
                     /locus_tag="ACF2SP_000452"
     CDS             19542..21284
                     /gene="phaC"
                     /locus_tag="ACF2SP_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HFD2358260.1"
                     /translation="MTHDTELSELMQAVAKKSLQIMTDFKEKPIPLSSLVSQYIDLTE
                     HFQNLIAVILKNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWIN
                     NPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQ
                     LMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRN
                     SMMELIQYCPRTTKVKSIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWV
                     NPDKTYANKSFYDYLNEGPREAISVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATK
                     DLSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRA
                     NDLIWSFFIKNYLRGKNPVPFDILYWNADSTNMPATMHSQYLRWMYLHNDLIKPGKIR
                     LNHTPIDVTNIDIPTFFLSTQKDHIAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINP
                     PTQQKYGYRTNSSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGRLTNSPDINHLPF
                     APIMDAPGSYVLKK"
     gene            21333..21716
                     /locus_tag="ACF2SP_000453"
     CDS             21333..21716
                     /locus_tag="ACF2SP_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358261.1"
                     /translation="MRKKNRILLASLFLLGSLNAFASQDLTVVNTSDDLGYVTIGSYS
                     CELTSSCAIPPDNSIRINSDIVEAACANTPVDCEIVFGVDSIKARVATGSFSLNEGIQ
                     YIAQSRWLNYSAKRLSKTVAEIYKR"
     gene            complement(21803..22222)
                     /locus_tag="ACF2SP_000454"
     CDS             complement(21803..22222)
                     /locus_tag="ACF2SP_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HFD2358262.1"
                     /translation="MKLIWIVLALILAFVVILYLFPQINFREMPKGLVEGQLPDDKPN
                     WVSSMVNESDSHYIAPLKVESMPQLVACIERKIPELKIQKQEASLLVGYRQSRFFHFV
                     DWLCIHADGNVISSATMGYSDFGKNRELVEKIRSLCL"
     gene            complement(22223..24424)
                     /locus_tag="ACF2SP_000455"
     CDS             complement(22223..24424)
                     /locus_tag="ACF2SP_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFD2358263.1"
                     /translation="MMTKTNEENLTWKEKQKQRARAWIKILLEKWFRIEVKGQYQANS
                     HSVIIANRASIIDVLLLSVFLPERLTLALHPDMFKKIWVKTLLFFADVIVVDPGSAQA
                     TRVLIRAIRAGKRCLLFPQGLLRQQEDSLKVFEGPGLILQKVGAEVIPVRIEGAEHSI
                     FSNSKNKHRIRIFPKITLHILPAQKLIQDENTGVDREGISLRLFQLISDLSFANSFKP
                     YVLFSALIEGVSIGAKNKTKIEDNNRIPLTYRQFLARCFILGRQIKKQTAIGETVGVM
                     MPTTIAGMVTFFALQAYRRIPAMLNFSMGFYNLYSACNTARIKSIYTARQFIETARLE
                     PLVEELQQAGIRIHYLEDFKSTIHLGNKLSGIFKGFFPTLSYRFLGDKISPDTTGIIL
                     FTSGSEGKPKGVALSHANILANCWQMTSRVDFTPRDVLFNSLPIFHCFGLTAGSVLPL
                     VNGLNCFFYPSPLHYKVIPGLVYQTGATILFGTDTFLTGYARAAGKHDFNSVRYIFAG
                     AEKVKPETIRYWSEAFGARIYEGYGATEASPVISLNCPLASVPGSVGMILPFMDFKVE
                     PVEGIVQGGRLKLCGPNIMLGYLDEDKPGVIIEPEDGWHDTGDIVTVNADGFITIAGR
                     AKRFAKIAGEMVSLTAVEGIAASIWPELLHAAVVQKSPRKGEQIFLFTEAEYADKVSF
                     IKKVKERGQSELLVPHMIYPACQIPVLPSGKIDYITLEKQFLEQKETMETV"
     gene            24579..25262
                     /locus_tag="ACF2SP_000456"
     CDS             24579..25262
                     /locus_tag="ACF2SP_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFD2358264.1"
                     /translation="MRVLNSCNKVRGIFTLTQKTIKTFCLILAILVLIIFAVVFHNHS
                     LEIIDYIDDLGWLAPVLFLITYCLATLLLLPTMVLTLAGGAVFGPVFGTLLNLLGATS
                     GAAFAFLITRHLVYDWFSTKKGEKLNKLIAGVDEKGWVFVAFLRLFPIVPFNLVNYGL
                     GVTGISFRLYLLTTFIFLIPAEIIYTYFGYVGMDALTRPGHLYRNGGIFLTGLAIFFL
                     LVIRIMKKR"
     gene            complement(25289..26104)
                     /locus_tag="ACF2SP_000457"
     CDS             complement(25289..26104)
                     /locus_tag="ACF2SP_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFD2358265.1"
                     /translation="MPTRVIEDKGDMTPSFGIDDRIFLGEGLFETIRVNSSKPSFAYM
                     HWERLGNSARQLGIPFEISFDDWFEHLIQKIQKDNLYHGGIKAILSGGPASRGLAERG
                     QVSQLIFQTFNYSIQKHPVRLISINWLRDKANPLYQLKSVNYLEAIIAQRQAIAVGAD
                     DALFFNTENHVTETTCANLFLIENNILYTPRVDDGILPGITRARLISHCQQHKMSVQE
                     ISLTKKRIEDADAVFLTNSLQGIRRVLSLDNIIFEVNHPIIDKLIFLLNQDES"
     gene            complement(26118..26303)
                     /locus_tag="ACF2SP_000458"
     CDS             complement(26118..26303)
                     /locus_tag="ACF2SP_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HFD2358266.1"
                     /translation="MSDLFEEDEEEVIEVVDDFDDVEVDTEIIEAPGVSLDARRRLEN
                     MLEEKRLRDELDDFVDY"
     gene            26550..27131
                     /locus_tag="ACF2SP_000459"
     CDS             26550..27131
                     /locus_tag="ACF2SP_000459"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFD2358267.1"
                     /translation="MSDQYKVALRNTIKQVRSKLSVSYRTASSNQICTRIRSLEQYRQ
                     AKRIALYFAVNGEIDLTFLWKSAPLQGKYCYFPVLNNDSTLSFLPATPSTPFKTNKYD
                     IPEPDVDIELALPVDELDLIIMPLVAFDAHCTRLGMGSGYYDRTLANKSKAHLLGVAY
                     QFQRVDYIEPRPWDVPLDAVITQKAIYWRNPLA"
     gene            complement(27141..27479)
                     /locus_tag="ACF2SP_000460"
     CDS             complement(27141..27479)
                     /locus_tag="ACF2SP_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFD2358268.1"
                     /translation="MKMITAIIKPFKLDDVHEALMEIGVPGITISETRGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIELALPDNMVEAAIEAICKSAYTGKIGDGKIFVYALEQVVRV
                     RTGEVGTDAL"
     gene            27624..27884
                     /locus_tag="ACF2SP_000461"
     CDS             27624..27884
                     /locus_tag="ACF2SP_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFD2358269.1"
                     /translation="MFDPKQFDELANKLFATLPTSLQNFEKDIQQKFKEVLQSTFSRM
                     DLVTREEFDVQCKVLARTREKLEQLQHQLEDFIKQQEIKNNK"
     gene            27949..29460
                     /locus_tag="ACF2SP_000462"
     CDS             27949..29460
                     /locus_tag="ACF2SP_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFD2358270.1"
                     /translation="MSLAFTKTRSTIGIVAQPVSVEVHLSNGLPSFTMVGLAETAVKE
                     SKDRVRSAIINSQFEFPCRKITVNLGPANLPKTGSGFDLPIALGILAASEQIPLTNLA
                     NHEFIGELALSGELRGVSAIIPAVLAAHKDNQHLIIANANAAEASLTGHQKVFTANNL
                     REVCDYLCQGTSLQSLPPKPDLLLNNYELDWSDIKGQQHAKNAMVIAACGGHSILLSG
                     APGSGKTMMAKRFSTLLPELSETQALECAAINSIRGKLPDFREWRLPPFRAPHHTASP
                     VALVGGGNPPKPGEISLAHHGVLFLDELPEFNRQVLETLREPLESGHICISRAAAQIE
                     FPAKFQLIAAMNPCPCGQWGNSQANCMCTPDRISRYLAKLSAPLLDRIDMQVTIHALS
                     QEELIKPNTHLEKQSLAIREKVTKMHEIQMARQDSLNANLNSKTCEMVCELGSEEQLF
                     LREVMSKLKLSARGYHRLLKVSRTIADMNSSKKVLLNHLQQALSYKQNLHLPK"
     gene            29572..29955
                     /locus_tag="ACF2SP_000463"
     CDS             29572..29955
                     /locus_tag="ACF2SP_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358271.1"
                     /translation="MILRLMLTLCCIVFPCKLLMAKQIMDNAIMIVKNCYDKSTDLSK
                     TFVPCINEEIEKLPDSKNFQIRMKLKSAEKDNNEKISILMVDKTGYMYYCIVTTGKTL
                     IINACAGEQGKPLTEGQMLSIDLPE"
     gene            complement(30069..30962)
                     /locus_tag="ACF2SP_000464"
     CDS             complement(30069..30962)
                     /locus_tag="ACF2SP_000464"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFD2358272.1"
                     /translation="MKHFLEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFY
                     ENSTRTRISFELAERHLAMSVVNLDLQTSSEIKGEAIEDTIRTLAAMGIQYFVIRHKQ
                     DGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIIEQKKRLDKLKIAILGNIKH
                     SRVANSFQCICSKLGVGDLVLISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQR
                     ERLLEDDHLDLDFYRNNFALTQKSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCI
                     LQQVTNGVYARMAILESLIAS"
     gene            complement(31012..31431)
                     /gene="ruvX"
                     /locus_tag="ACF2SP_000465"
     CDS             complement(31012..31431)
                     /gene="ruvX"
                     /locus_tag="ACF2SP_000465"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFD2358273.1"
                     /translation="MPRGVYLGFDFGYKRIGVAVGQRLTCSASPLSTIEAKAGIPDWN
                     TIQKVITQWNPQALIVGLPTCIDDRELYTTSAARHFAKQLHKRFSLPVHLVDERLSTV
                     EARGYLFEQGGYRQIKKAEVDSIAACVILEQWLQQSE"
     gene            complement(31424..31987)
                     /locus_tag="ACF2SP_000466"
     CDS             complement(31424..31987)
                     /locus_tag="ACF2SP_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFD2358274.1"
                     /translation="MAIISSLANHLLIAMPSLKDPNFERSVVYLCEHNEQGSVGLIIN
                     RPLQFPLSIVFEQLQIEPIRVEKNGLPLLFGGPVQPERGFVIHKQMGGWRSSLFLQDE
                     VTVTTSNDIIRAIAYDEGPKDVLITLGYAAWTEQQLEREIMSNTWLVCPYKSEILYEV
                     PFEERWEYAGLTLGIKMNQLSSDAGHA"
     gene            complement(32289..33053)
                     /locus_tag="ACF2SP_000467"
     CDS             complement(32289..33053)
                     /locus_tag="ACF2SP_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/III secretion system protein"
                     /protein_id="HFD2358275.1"
                     /translation="MIKRWLLVILLISGNAFSQQMITKVIELNYLQADKVIQLIQPLL
                     QTDEKVSGSGQTLIVNVSPQTLTQIRNVLHKIDVPPVVFNITVYQGDPEWLSSQDGND
                     VSYSTQPQYEVQRSQSVKVMSGESAFVATGEQVPVVTSVGVGLLATGVSYEQHNIQNG
                     LLVHPVLQGSQVKLTVRRVREQENVESDQQFDNQQIDTTIMAPLNKWVPLGTAEGVQN
                     TDSSSTSYTAGRPFSQNSTLYIKVSVVKSTPSGTTK"
     gene            33244..33915
                     /locus_tag="ACF2SP_000468"
     CDS             33244..33915
                     /locus_tag="ACF2SP_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HFD2358276.1"
                     /translation="MGSIFNMFGPSPIKPIEQHIRKAHQCAKQLYPFFEAVLKNDWDT
                     ANKIKDKIIAIEKEADLIKRDLRLHLPTGLFLPVARTDILELLSAQDRIANKAEDIAA
                     LIISRQMSIPKKLIPSFMPFLSRCLDASKQACTAINELDELLETGFRGSEVKIVEEMI
                     VKLDEIEHDCDERLADIRHKIFELEKELPAIDVIFLYKLVQWIGELADHAQTVGGRLQ
                     ILIAR"
     gene            33925..35178
                     /locus_tag="ACF2SP_000469"
     CDS             33925..35178
                     /locus_tag="ACF2SP_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFD2358277.1"
                     /translation="MDYSIYLLFAALILCFLMTWGVGANDLANVMSTTMGSKAVTVRQ
                     AMLIAIIFEFAGAFLGGEGVTETMRDGIINTSQLSNEPLILIEGMLCVLFACTIWMNL
                     ASYLGVPVSITNALVGSMVGFGTIVLGPDAIHWNQVARIAIGWVSSPLISGITAYALF
                     ISIQQTIFVKSNPLTKAKLYIPIYLFLIGFILSFITVFKGLNHFDIHLNLKQDLAVTL
                     ATSIIITILGMIFIKRIPEYHKIRRRERFIQVEKYFAVLMAMTACAMAFAHGSNDVAL
                     AVGPLSIVHSLVMHSNQIFDADNYPSWIILLGCVGVVTGFLMYGRKVIETVGSSITAL
                     TPSRAFAATLSAATTVVVATSTGIPVSATQTLVGAVLGVGLARGIGALNLIVIRNIFM
                     SWVLTLPAASMLTILSYKLLHALLG"
     gene            complement(35219..35665)
                     /locus_tag="ACF2SP_000470"
     CDS             complement(35219..35665)
                     /locus_tag="ACF2SP_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358278.1"
                     /translation="MKNPSILQLNNPALLNKAMQLKSTGKVAISQNQLTYLKIHDEWI
                     HELFPLLQNEQIRKPDYFGNESIGAHISIIYPEENTTINPKELDQEHRFKIKNIIKAN
                     LELKKYYAIIVEASSLVRLRKAYGLPENLYFKGYSIHFHITVGVEY"
     gene            complement(35764..35949)
                     /locus_tag="ACF2SP_000471"
     CDS             complement(35764..35949)
                     /locus_tag="ACF2SP_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBU_0585 family protein"
                     /protein_id="HFD2358279.1"
                     /translation="MNTDDINKAYVSPYDKFLYEFDATHKKSASQLHEIKKHERIFKM
                     RDDKDYKIDQSEIWEGF"
     gene            36047..37027
                     /locus_tag="ACF2SP_000472"
     CDS             36047..37027
                     /locus_tag="ACF2SP_000472"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFD2358280.1"
                     /translation="MNLKKAELHVHLEGTISPDLAKKLAKRNHLTLPDGLIALDEKSY
                     LSKDFLDFLKVYDTLASVIKNPQDYYDITFDYLKSNAQEHAIYIEMMYSPDHAEQSSG
                     IPSKEHLAAIQQAIDDAKSQFDIVGRIIITAVRHFGAEACERVAKQASIDKFPCVTGF
                     GLGGDEAKFPPQLFSKAYQIAADSGLECTVHAGEFDSAKGMEEAMKTLPIKRIGHGVR
                     VIDSPDIMAMVKDQGIALEVCPTSNIFLGLFKNMNSHPFPKLYEAGIKVSINSDDPPF
                     MSTTLAQEYKRVQKAYGYSDNTMNNITRMAIEAAFVDEKTRKELLTKLEQ"
     gene            37258..38616
                     /gene="gorA"
                     /locus_tag="ACF2SP_000473"
     CDS             37258..38616
                     /gene="gorA"
                     /locus_tag="ACF2SP_000473"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFD2358281.1"
                     /translation="MKTKHFDLIVLGGGSGGIASAVRAAQYGAKVAVIEQNHLGGTCV
                     NLGCVPKKIMYNASSIAETLHKSPDYGFFLENNAKLDWKRLVNKRNAYIERLRENYAK
                     RFSQHKITLIQGKGIFHDQSSITIDHTIYQAEHIIIATGGEPALPAINGIKHAIDSDG
                     FFSLTKLPAKVAVIGSGYIGVELAGILNSLGSETHLLMRGTRPLSRFDHMIGDTLMEI
                     MQKQGICIHQNHKAQAIHLHSDGRKSILCQSGSIIENIDVIISAVGRKPRTGNLNLDK
                     INVNMDDKGLILVDAFQNTSVKGIYAIGDVTNAPALTPVAIAAGRRLADRIFGNQPDA
                     CLNYDNICSVVFSHPPSGSVGLTEHEAIEKYGKSKIKIYQTRFIPMYDALSVDKTPTA
                     MKLVTLGKKEKIIGLHVVGYSADEMLQGFGVAIKMGACKKDFDNTIAIHPTSAEEFVT
                     MV"
     gene            38623..39159
                     /locus_tag="ACF2SP_000474"
     CDS             38623..39159
                     /locus_tag="ACF2SP_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFD2358282.1"
                     /translation="MNYSTRKFEDKYPELGERVYIDPQSTVIGNVTLGDDVSVWPMAV
                     IRGDVNYIQIGHSCSIQDGAVLHVTHDGPYTPGGRPLILGQGITVGHKALLHACTIDD
                     YCLIGMGSIILDSAHIQKHAMIAAGSIVPPGKILKSGHLYLGSPAQAVRKLTTKEIEQ
                     IEYSAGHYIRLKNKYLTY"
     gene            complement(39345..39460)
                     /gene="rrf"
                     /locus_tag="ACF2SP_000475"
     rRNA            complement(39345..39460)
                     /gene="rrf"
                     /locus_tag="ACF2SP_000475"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        12230 a         8091 c         7210 g        11961 t
ORIGIN      
        1 cgaatatttt gcccatcaaa agcacaaggt tctgttgacc tgattggatt aatatccaga
       61 cctccccttc ttgtatatcg cccataaaca gccaatgact caggttggca aaatcgcata
      121 atgtctgcaa atatccgttc aatgcactgc tcatgaaatt cattatgatt tctaaatgaa
      181 atcaggtact taagcaaccc ttcatgattt atctttttac ctttataaat aatttgaacg
      241 cttccccaat ccggttggtt ggtaaccagg cagtttgatt taagtaaatc agaatacaat
      301 acctcttcaa ctaatttaga cgaggtcgaa agaaaatcag gcattacatg atatacagag
      361 cattcaatat caagcttatc aaggcaaata ccatcgaatg cagcatagat agtttgacta
      421 gcgatttccg acaaggggta cacattaacc gccacttcag aaccaatgca gtgacttaaa
      481 tctttgctga tagtttgcac aacggtttcc acatcaggga aagtactatt attcaatgag
      541 ttaaaataga gttttaatga ctttgattca ataatacacg gggaatgaca atcataagaa
      601 ataacagcca tcgcaacaac aggtttacct ttggagttta accaggagac ctcataatga
      661 ttccagcaat caaaaccata gaaggtggtg gagttgggat ccaaattaat ttcctggcgt
      721 tttggggctc tggggatagg atacagcctt ttaggattat agtagctgtc ataatttgca
      781 gtttgtccca gctccgattg atttgctata ttctgataaa tactttcaag ttccttattc
      841 attatggatg ccttttgtaa ctctaaaaaa caaagtgatt ataatctatc atgttaccta
      901 agggtcaatt ttttcttaaa aaaaaaaagg aaatagccta tatttaatta aagatactga
      961 aattagcatc taaaggagcc catttgccta tgattaatca acagatagaa atatttttga
     1021 aatctcctgc ttttgccgta gttggtgcct cttccaatag ggaaaaatac ggcaacaaag
     1081 tattgcgatg ctatcttcgg aatggcaaaa aagtttatcc tgtaaatcca catgaaacgc
     1141 tgattgaaag cttgcctgtt attcattcag taaacgattt acctgataat gtacacagta
     1201 tttctattat cacccctcca tccattactg aagaagtcgt caaggaagca gtcaaaaaag
     1261 gaatccaaca tatatggatg cagcctggag cagagagtga ctcggccatt caatatggct
     1321 tggatcacaa gcttaatgtc atttctgggg gaccttgtat attggttgtc atggggtatg
     1381 aagagaatta aggcaaaaca aaaagctatt ttgtagtcca aaatagctta tttgattttg
     1441 tataatccca gccgattatt tagaactcct gaattgcttt aatgattcat tgaccacact
     1501 ttcaagagtt ctatgttctg catatttttc tcttaaaaag ctggcatcac tccctttatg
     1561 agttatttca taaatatgct ccaaagccat agtactattt agctgattag cataaggctt
     1621 taatcggcga agtgttgtta aaatatcttc tcgaagcaaa atttgctcat atgttttggg
     1681 gtgcaccaga gtgccatcca gaccgaatcg gcatgcttga aaacgattgt aattgtacac
     1741 aagataatca tcttcgtgag gcagaggttc tttattttcc aataaataac tacaaagcgc
     1801 ttgcatataa caagctaaag cggctgctcg ctcaaccgta aggggactat cacacacccg
     1861 catttcaatt gtcccaaatt caggtttagg tcttaaatcc caataaaaat ccttcatact
     1921 tttcactatg ccggtatgct ccattttttc gaaataaccg agtgagaatt catcccaatt
     1981 taatacaaag ggagcccttc cacttaatgg gaaagcaaat accgaattca atcgagcaga
     2041 gttatacaag gtatccttac tctgaacgaa gggtgatgaa gcagacaatg caataaaatg
     2101 agggatataa cgatttaaag aatgaagtaa aaaaagcgct tgattaccat cctcacaacc
     2161 aatatgtata tgttgcccaa aaatagtaaa ttgcttggtt agatagccat ataattcaga
     2221 cacctctata aacctggttt tattaaaaat tctctgatta gaccacattt gaaatggatg
     2281 ggttcccccg ccacaaatac caatatttaa tcggtctcca actgtcacca gggtatcacg
     2341 tatatgaaac agttgtttca gtagttgatc atattcttca tgcacatctg tcgctatttc
     2401 taacatactt tctgtgattt ctggcgtaaa actgccggga aaagacgaac gtcccaagag
     2461 ctccaatata tcagggctag cggcagtcaa atcaaaatta cttaagctga ttaattgtaa
     2521 ttccagctca acccccatag tcagtaaatt agatgttttg aatggttgta aaggcatttc
     2581 aaaattcctt gattgcagag atctcgttcg ctttaattaa agcacgttga accattattg
     2641 gacccaatat ttccaaagtc agagcaacca tagccagcgg ggccagtgta tctaccaaat
     2701 taataccaat atatcgactc tgttctaaaa tcaaaatgac aaaaacagag atgggggtca
     2761 tagccaaacc agtcaagata ccttttttaa acgaaatccc actaatatga gcaaataaac
     2821 taatcccgat aattttagct acgaacctta cagttataat tgataatcct atccatacac
     2881 cagttactac cctttgccat tctatggttg ctgcaataaa tacaaacaaa agtaccgata
     2941 acaaatcacc taaagtgcca aatcctggct gtgaaggaga aaagaccatt cgcccatggc
     3001 gggaaaccaa tccaaaggtt aatgtggcta aaaccgggga caattttaaa ttatgggtta
     3061 aaataaccag cagtatcaca gcgcaggcaa atgccaatgt attgtcttga caggtgcttt
     3121 taactgtttt aagcaaaatg ggcattatca ctccaaaaat aatcccccaa atagttgaaa
     3181 tggctgtcac cgttaagctg gcataagctg cttcccaaag atttcctgaa gttctgaaaa
     3241 caaccaagcc aatgatgact ttgaataaaa atactgccag cacacaattg atcacggaga
     3301 gatgtaaaac acgttctgtc acttggcctg aactacgttg ttcattaatc accctgacta
     3361 cagtggctgg tgaagtcgcc atggacaacg aggccaataa aaggcaggtc gaagtttgta
     3421 acccaaagga tttcactaaa atataaacca ggcaaaaggt taaagtcgct tcggtaatac
     3481 tggatgctgc aacccagggg ttataactta accagcgtaa attaattcta tagcctgatt
     3541 caaacaagat tagcccaaaa ccaatattgg ctaaaagtaa aatagatggt ggttgaatag
     3601 acggtaataa tcctatttga gttgatgcta gtgaaaatcc tacaatcgca tagatactga
     3661 tatgaggcaa ttttgcccat cgatatccaa gttcccctgc aaaccaagct atcaataaac
     3721 tgataggcca agccaagtcg gttataattt ttccaacatc aatcatccat acctcctatg
     3781 aaaatccgac cttactttat aacacacatc gatttgtttt gcagaattga agtattttta
     3841 agacaatata aaataatcag gacttacgaa aaatcctaag gtcgaggcaa aaaatgtttt
     3901 taatgaatga gtttagataa aataaatggc tgaattaaaa aattttttaa caaagagatt
     3961 gaggtttttc gtgagtactg atagtttctt gtccaattag gaaaatcatt gctactaacc
     4021 acattaacaa gaaccaaaaa gaaatctcaa caacttctta ttttaatagg cttcttatta
     4081 gggatgtcac cagtcatggc tttagaaatt attacaatag aaaaaaaaga aaactaccat
     4141 atgctaaaaa ctcgggctga aaatattcaa tttcccttga atcgagaaga tcaagattta
     4201 atagaagcga tgaaaaaaaa attatacgca ttgggagggg ttggtttggc agcaccacaa
     4261 gtcaattccg ccaaacaaat tatcgcagtt tacatccctg aagaagctca gcttttacgt
     4321 gataatgcga aaatctatcc aatgcacatc ctaatcaacc caaattacga gcccttgccc
     4381 caggctcgta ttattagcga ctttgaaggt tgttattctg tatccagtaa agcagggaaa
     4441 gtgccaaggt atgatgaaat tcagctaaag tactatgatg aagaagggaa tgttcaccaa
     4501 caaatcgaaa atggctttta cgcccgtgtt ttgcaacatg aaattgatca tctgaatggc
     4561 gtattaatca ccgatcgttt aactcccgat tgtgtacaag ggactattga agaaatgatg
     4621 actattcgcc gtgctgaatt gcctttagaa aaacgaaaat tatttgatca aatcatggca
     4681 aaaaaactta aaaaataagg tcgagtttcc aaattatctt ttatgaattg acttcaatga
     4741 taaactgccc tgggtaattt ttttgcttgt tcaacccagg taacaacttg agataatcca
     4801 ggtacatgtt gcgccaaatg acattgctca tttttctctt ttaatgagct ataaagttgt
     4861 tcaggattgc aatgtgctaa ctctgggatt gagtcaatac cagcctgttc cagaagctca
     4921 gcatattctt cattaatgcc atttactcgt gcaagatcag cttgattggt ccatttcaaa
     4981 atcaatttat gactgattcc cgtttgctcg gctactctaa aacgcccccg gcgaccacta
     5041 caaactctta ataaatcttc ctgactttca atccctactc gctttagcat ttcagcatat
     5101 tttgggccta cgccttgcag ctcaattaac ttgtgcattt tatttccagt cttctactac
     5161 gatattacat atataaaatt atagaatata atgatcaatt attaaccaag acaaatgttg
     5221 aaaaaattta ggcctcacgt attaaaaaaa atcaattcat ttggcaaaat gaaacaattt
     5281 cattatgata attactttcc tttcagtccc tggtgaggta gaaaatgcgc aagcacaaga
     5341 acaaaatggt caggatattt ttattggctt taggactatt ttccagcacc accattaatg
     5401 cttggtatgg ctcagggata accattggtg ttgccagagc gaatgttcac tattacggag
     5461 atgatatccg agtatatgac ccgtggggct atggatacta cgacagaaat tattactata
     5521 atggagtaag aaacattatc gttcctagtg taattgtacc gaatgtagtg atcaatgtac
     5581 cagttcaaag gtattacgta ccctcatgtg aggaaatgga agtttgtgat ccttatggac
     5641 aatgttggtt agagcaattt tgcagataaa ttaataactc aatcaactaa tgttttggga
     5701 ctcagcccat tatgggatga gatattagca ggacttacaa aaatcccaag atcaagacaa
     5761 aaaatgcttt taatgaggga gtttagataa actaaacaac cgaattaaaa acattttcta
     5821 acaaagagat tgggggtatg tcctgattat aattttttaa ggacaaaaaa tgtcagaaac
     5881 tattggttat gccgcccaca aagcgggagc ccctttaaaa cctttcactt ttgaacgccg
     5941 ccctgttggt gcacacgacg tagcaattga tattctattt tgtggtatct gtcattccga
     6001 tttgcatcaa gtcaataatg attgggcagg agcattctat cctatggttc ctggacatga
     6061 aatcattgga cgagtcaagg aggtaggaca tcaagtccgc cggttcaaag caggagattt
     6121 agtgggcgtt ggctgtatgg ttgactcctg ccgtcaatgc acctattgcc atcagcatga
     6181 agaacaatat tgcaaatctg gagcaacttt tacctataat agtcaagaca atcatctggg
     6241 aggggtgacc tacggggggt attccaaaca tattgtcgtc actgaagatt ttgtattaaa
     6301 aattgatgaa tcacttgatc ctgccgccgc cgcaccatta ctctgtgccg gaataaccac
     6361 ttattccccc ttgaaaaggg ctaaagtagg taaaatgagt aaagtaggaa tcgtaggcct
     6421 gggtggctta ggacatatgg ctgtcaaatt agcccgtgcc atgggggctt atgtgactgt
     6481 atttaccact tctataaaca agataaaagc gggtaaagat ttgggagctc atgatgttgt
     6541 cttgtctacc gacaacaatg tcatgcaaac actagccaat caatatgatt ttattcttga
     6601 tactgtttct gccaaacatg atcttggcca atatttaaac cttcttaaag tggatggaca
     6661 actaacccag gttgggcttc catcagatcc acttgaagtt accatgtttc ctgttatttt
     6721 aaaaagactg agcattaacg gctcattgat tggcggcatt aaagagacac aagaagtttt
     6781 ggatttttgc gccaggcatg ggatcaccgc agacattgaa ttaataaaaa tgcatgaagt
     6841 aaattctgcc tacgaaagac tgttaaaagg agatgtgaaa tatcgatttg ttatcgatat
     6901 gtcaaccctg gattaaatga acccggtata aaaggcttcc agttggcctt ttataccacg
     6961 gagcaaatat tattcctagt ttttaatctt ggggaaaact ttaattttgg ttataaaaga
     7021 atcctgtacc ttttcaatca aggcatcaaa ttcaacaaat tgaattattt cgccttcatt
     7081 gggcaatttt ccgtggcgcg tgataataag tccatatagt gtattcagtt cttcttcatc
     7141 tggcgataaa gtaatatcgc aatcaagagc ccgttctaga gaataaaggg agcaatctcc
     7201 ttttgtcgtt atagttccgt ccgaattttt tacccaatct tcatgtgttt tatgaaactc
     7261 gtctttaatc cgcccaatca ggatatggag caaattatcc agagttacaa atccaagtaa
     7321 actcgatttg tgtttgtata ccaaagcaaa atgaggcata ccagctctga atttacgcag
     7381 caaatccata gcaggtaatt tatgactgac tttaagtact ggtcgcacat aaggctttaa
     7441 tgtattgata tttttctgga gcaacaaatc ggcaaataaa tccttaacat ggatgacacc
     7501 aatcatttcc ttagtcccgg aatcatagac aggataacga ctataacgat attttaaaat
     7561 tttatcttta atcgtttcga atggctcatt tatatctatt atgatcattt catcttcagg
     7621 acgcattacc tcagtgacat ttaactcagc caaatcgaga gtatgctcca ggatttcacg
     7681 ttcatcctct tttaattccg attgcagatg ctgtccgctt aaaattaatt taagttcctc
     7741 acttgaataa aaaccttcgt attcattttt tatatccatg ccggacagtt tgagaaaaaa
     7801 attggaacag gtatttaaca accaaatggc aggatacatt atccagtaaa accaatacaa
     7861 cgggactgca gtccatattg aaatcaattc agattgccga atggcaatgg acttgggcat
     7921 gagctcaccg attacaatat gtagaaaaga aataagaaaa aatgcaataa aaaaagaaga
     7981 tatagtaaca atatgaaccg atgaaatacc caaagcaaca aataatggcc ccaataactc
     8041 tgcaaaagca ggctctccaa tccaacccaa tcctaacgaa gcaagagtaa tgcctaactg
     8101 acatgctgat aaatacgcat ccagattttg atggacttgc tcaagaatgc ggcctcttag
     8161 cccgtaattt tctttgatgg cttgaattct cgtagccctg agtttaacca tcccaaattc
     8221 ggccgataca aaaaatgcat tgagaagcac cagcaggaat gctataaaaa ttaacagaat
     8281 gttatacatt aaaaatagtc cggtttatga tttaatttca cagaacttac gaaaaatccc
     8341 attctcttcg ttaaaaatat ttataattcg gtcatttggc ttatataaaa tccctcatta
     8401 aaaatatttt ttaccttggc cctctggttt ttcgtaagtc ctgttttcta ttcagtcgta
     8461 ccttcactaa aaacattcac tctctacaaa tgtgatcgaa tcctttatgt taatagaata
     8521 tcgcagtcct ggcgagttta aaatgactaa gccagaaccc aaagataaaa taattatcat
     8581 ccgctttaat gacaaattat ttaatcaaag caaccgtata gaaatgacac caaatatcag
     8641 taaatagcag ccccctattg acaaatagaa gcacaatttg ttaattttac tcattaaaag
     8701 acaaaattaa ctcaacctga aaagattatg aatcgtccag acgacaaaac atgccatgac
     8761 ttttatgcaa attcttatgc caatgttttg ctttgcggcg cttcccagtc tatagataat
     8821 ctttcaatca ttattattat cttgccctct ttcccctaga gcggtcagct gttgctaatt
     8881 ctctgcctct ctagggaggc ttccaaagag ttagcaccaa ctgatttcaa ctggagggcg
     8941 tcatgaatca aaatagtttc aaaccaaaga ataatttcgt ctcattggtg atgtgggcat
     9001 tacctttatc tttttttact tttcaattct tattacgtct ttggcctgga ttaaccatgc
     9061 aagacattat ggcgcaattt tcaattgatg ccgcaagctt tggccttatc gctgcattct
     9121 attattatgg atacgcagga atgcaaatcc ctgtagcctt actgctggat aaatttggag
     9181 cccgcttcgt tgtatttgcc tttgcaatgt tgtgtggtat tggaacttta gttttcatag
     9241 gtaccaataa tttttacttg gctctgtcag gcaggttttt gataggtgta ggctctgcgg
     9301 ctggttttct tggcgtatcc aaagtcgtat cagagtggtt cagccattca cagtactcca
     9361 gaatgatcgg tttatccttt agcgtaggct tgttgggggc aatctatgga ggaaaaccgc
     9421 ttgcccacct gatacagaat tttagtggac aacaagttgg tttggctttg gcggttttct
     9481 ccatcctctt aggatgttgc gcacttttga tcttgcgctc tcccccaaat aatactcact
     9541 cagtaacaaa aacaatgaat ttgtctcact gttcctcttt attttcatcg tggatcatct
     9601 gggctcttgc tcttgctaat ttattaatgg tcggtgccct ggagggattc gcggacgtat
     9661 ggggagtacc ttatcttatc gccaaatacg cactgaacaa aactgatgct gcttttctca
     9721 tctcgtttat ctttatgggg atgatatttg gcggtccttt actcgctttc ctcagccaaa
     9781 aattaggaaa ctactttgta atagccctct gtggtactgg tttggccttc ttgttcctaa
     9841 ttcttcttta caccgctaca cttcactggt ggcagctggg cactctcttc tttgcaatcg
     9901 gcctgctgtg ttgctatcaa gttattgtat tcgccgcagg ttctactttg gttccttatg
     9961 aaggtttagg cgtcaccatc gccttcttga attgcatcaa tatgttaggc gggtcttttt
    10021 tccatacttt gattggtaaa ttaatggata gcagttggag cgggcaaatg agcgctaatg
    10081 gacttaaatt atatgaaatg gaatcgtatc aatgggcttt gtcactcatt ccattctgtg
    10141 ccttatgcgg cagtttgctt attgtattaa ttcatatcaa aacaagagct tatgcagccc
    10201 cagagcttat gaaaaagact tcattgacgg gagtgtagct atggaaaaaa ttttttggtc
    10261 taatccctac cagcatacac tcgataccaa gataatctca attaatggca acgagattct
    10321 attagaaaaa accatcgcgt tttcattttc aggaggacag gaaagcgata gagcatttat
    10381 caatggcctt gaagtactca gttcacgaat agatggaaat cttatttatt ataccctgcc
    10441 tgatgaccat aatcttgcgc ccaatgatgt aatcaccatg actatagatt ggcctcgaag
    10501 acaccggctg atgcgtcttc attttgcagc agaactgatt ctggaacttg taaccagaac
    10561 cagtatgctt gaaaaagtag gagctcacat ctcagaatcc aaagctcgaa tagattttaa
    10621 agcgaatttt aatatctctt ctatttttag ccagctgctt gatgaatata atgccatcat
    10681 tagacaagat cttgttatct ataaaggata ccatgatgtg acaaatcaac gtcggttttg
    10741 gaaaattgac ggttttgctg aagtaccttg tgggggaaca catgttcatt ccacttctga
    10801 agtgggtttt attcgtctca aacgctcgca tcctggcaaa tctattgaac gcattgaaat
    10861 aaccctgatg gatgatagta gataatgcac ccctcgcata agctgattgt tttgctttgg
    10921 ggcaaggtga gaatgttatg gggcaatgag tttatataca ttgaatgaac gttgctgaaa
    10981 cgtttggagc gtgacagtaa aataaacaag gcgggttatt caggaggcca ggtcaagcgc
    11041 taatcgagac aaaaatatag aaaaagaata caaaatcgcc taaattacag catttgactt
    11101 tgtcgccatt aacctgtgag cgttatttat catggaactg ccctatcatt tctctactgc
    11161 ctatgtgtca aaaccagagt ttatagaagc actttctgag gagttaggcg aggtatccaa
    11221 taaaataggc tctctcgttt tttctcctca tcagaagctt gatgtttgtt ttgcactgga
    11281 tgtatggctt gaacctgaga tagtaacctt tcaatccatt tcagaagcag caaaaatatt
    11341 gaggcaagcc ggaaaattct ggtatttgca ccctgttact gaaattcgcc gatcgcgctt
    11401 gattgaggaa caacttcgca agtgcccatc actaatgtcc cattttccag ttacaagtga
    11461 cattccaccc ataggtgtat ttagtcttct tgatagaaac actctactct ttagcgccaa
    11521 acgactaaaa aaatggccgg atggacaatg ttattttatt gaagacaaag tcaatccccc
    11581 aaaccgggct tatttaaaat tatgggaagc cttgttcctg ttaaataaat accctctccc
    11641 tggtgaaaca gttttagatt tgggcgcatc cccgggaggt tggacatatg tgatgcaatc
    11701 actcggaacc caagtcactt cggtcgacaa agcagaactg gatacaaaaa tcatggcgct
    11761 tcccagagtc agttttttaa agcaaagcgc ctttgcccta gatcccgaac aactcacaga
    11821 gtcttatgac tgggtacttt ccgatgttgc ctgctatcct gacagagcct acaccctcat
    11881 caaaaaatgg atagcatcaa aaaaagcaaa acagttgatt tttaccatca aactacaggg
    11941 gcaaactgac ttaaacacaa tcaaacgatt tcaatccata cctaattcca aagtaatcaa
    12001 tctgttttat aacaaacatg aagtcacgtt tttttatcca gtataaacat aaccaggatt
    12061 tagtgattat gtctgcaaaa atatcttaat gacgagctaa tatttaattt tctatgactt
    12121 aagaccttga aaaatcaaat gagagaattt tttctatgct tggttgtttt aaggcgagcg
    12181 ccaacaatcg atcaaccccc agggcaaccc cgctgcaagg aggtaaccca tattccaggg
    12241 ctttcaaaag atatgcatcg gcttccattg ccgtcaatcc cttttgcttt ctatgttgct
    12301 gatccagcat aaaacgcttt ttttgcgcct cgacatcagt caactcatga aaaccattag
    12361 ccaattcaac tccctgatag tacacttcaa agcgctcagc caagccctct tttacttgtg
    12421 ccaaggcagc ctgggaggca gggaagtgat acacagcaac gggggttgat tcttctgcca
    12481 aaaaaggctc cacgacatgg ctcattaata aaaacagata ttgatccctg tcttgttcgt
    12541 taggagataa gacaccatcc aaatcatgct gtgctaatac cttgttgaat tgttctatgg
    12601 atgtagaaaa agggtcaatg ccgcaagcct cttcaaaagc ctgttgatag gtttttctga
    12661 tcataggccg ggatttaaga ataatttgta ataataaatc cacttcatcc atcaaaacat
    12721 gatgatcaat atgtaattga taccattcaa gtaaagtaaa ttcaggatta tgccagcgcc
    12781 ccagttcgtc atcacgaaaa actcgcgcta attgaaaaat aggcccgctg ccagcagcta
    12841 acagccgttt catatgatat tccggggaag tttgtaaata gcatgtttca cctctgaaaa
    12901 tcgctttgat attgctcagg taaacatctg taaccccatg ccgcgccatc acaggggttt
    12961 ccacttccag atattggcgt tcagtaaaaa attggcggat tttacctatt agctcggctc
    13021 gcgcacgtaa taattgaatt gaagctgagg gcaaccagga gttatcagac atgatcaata
    13081 aaatattccc aactccaggc gggcagcctc tgtcatgcgg tcttgagacc agggcggctc
    13141 ccacaccaac tcaacagtgc aatcactgac tccttcaacc tgattgactg cctgctccac
    13201 cgtaccggga aaggtttgcg ccacagggca ccccggggtc gttagcgtca tctgaatatg
    13261 aacatggcct tcgtcattaa cggcaatgtc ataaatcaag cccagatcat aaatattgac
    13321 cggaatttcg ggatcaaaaa ctcccctcaa cgcggtaata acgccttcct ttattgattc
    13381 gttactctgt tttttcttaa aaccaaacat gagctgcctc tggtctattt tgcaatatac
    13441 agacaaactc tttcacgagc tctgccacta ttgccataac attattctgt tttcaccact
    13501 tctgtttctt tgtttaaagc cgcttccaaa gtatgccacg ccaaagtggc gcattttaca
    13561 cgcgcaggga atgctttaac tccggctaga actgttaatt tatccatgga aaccaaagaa
    13621 tcttcttcct cttgtgttag cattcgatga acacgatgaa atagttcatg agcttcttta
    13681 attgatttac cctttaacgc atcggtcatt aacgatgcag aagcttgtga gatagcacag
    13741 ccacatccaa taaaactgac atcacgaatc aaatcaccct gcaacttgag gtaaacagtc
    13801 agtttatctc cgcataaagg gttaaacccc ttcgcctcgg tggtcgcatc ctccatcgca
    13861 tggtggttac gaggattacg attgtgatcg atgatgattt cctggtaaag ttcccgcaac
    13921 tccatactca tgcgaatacc tctttaactc gttgtaaggc ttccatacaa taatcaattt
    13981 ctttaaacgt attataaaag gacatggaaa tacgagaggt cgcagcaaca tcgtagaaat
    14041 ccatcaaagg catagtgcag tgatgtccgc tgcgtatcgc tataccttca ctatccagaa
    14101 tagttccaat gtcatgggca tgaatttttc cgtggacaaa agagataatg ggtactttgt
    14161 tggcagccgt tccaataata ttgtaccctt ttactgcttc aatagccttg gtagcatagt
    14221 ttaacaattg tgtttcatat tcagctatcg catccaaatc caatgaccaa atataatcaa
    14281 ttgcagcagc caaaccaatc gcccctgcaa tattcggggt gcctgcttca aatttatggg
    14341 ggatagccgc atattccgtt gcttcaaaac tcacggagtt gatcatttcg cccccgcctt
    14401 gataaggagt catgctattt aataattctt cttttcccca taaaacacct attccagtgg
    14461 gaccatacat tttatgtccg gaaaaagcat agaaatcgca atccaaatcc tgaacgtcca
    14521 caatcaggtg agccgttgcc tgtgccccat ccagtaacac tttagcccct acttcatggg
    14581 ccatcttaat cattgtttta acaggattaa tggttcctaa agaattggag gcgtaattaa
    14641 tcgcaaccat tttagtgttt tcatttaact ttctttcaaa ctcttcaagt attacctcgc
    14701 catttaaaga aataggggca acttgcagct tgcaacccat tttcttgcag accatttgcc
    14761 agggaactat attggaatga tgttccatat gcgttatcaa aatttcttca tcaggtaata
    14821 tgcgaggggc tacgagactc tgagccacta aattaatggc ttcagtggta ccacgtacaa
    14881 aaatacattc tctcggtgac ctggcattaa taaatcgttt tacctttgct ctggctgcct
    14941 catacatttc cgtagccctg acactcaagg catgaactcc acgatgtacg tttgaattat
    15001 ccttttcata aaattgagca atagcatcaa tcacagcctt gggtttttga gtagtagccg
    15061 cgttatcaaa atacaccaaa tcatactcat tgattttctg atgcaataca ggaaaatctt
    15121 tcctgatttt attgacatca aaggatgtca ttagggaagg attagtactc atttgcgtac
    15181 cccaactgtt gagttaataa attacccatc caatcagcca aatcacgatt tggaataagg
    15241 cgcaaattat cattggcaaa cgcctggatc aaataatgag aagcttcatc cctgctaata
    15301 ccgcgtgttg ccaggtaaaa gattgcctct tcatccagtt gcccaaccgt agcaccatgt
    15361 gaacataaaa cgtcatcagc gaaaatctct aattgcggtt ttgtatcaat ttcagcattt
    15421 gcggagagaa gtaaattttt attttgctgc ttggcatcag tatgttgcgc attcttggct
    15481 acaataacct tgccattaaa tacagctctg gaatgtccct ttaaaatccc tttgtaatct
    15541 tgatcgcttc gacagttagg caccaaatga tgaaccacag tatgatgatc tatgtgttgc
    15601 ccatcccccg gtgcgtaaat tccattcata aggcattgag cctgttcttc ctgcatggac
    15661 acgctaatat cactgcgtac taacttccca cccaaactta aagagtggct ggaaaattga
    15721 ctggcggcag attgcctaac cgataaatga cctaaatggt aagctgattt actttctttt
    15781 tgaattttat aatgattaag ttttgcgcct gctccaagaa acacttcagt aatactgtta
    15841 gtgaaatagc aaatgttttc ttcgccatga tattcttcaa tcagagtagc ctgagattcc
    15901 ttttccataa ggatcagatg tcttaaatac atagcctggt tcgtttgatc ttggatatgc
    15961 tttaaaacaa tgggttcttc caaacaaatt ccctcaggaa tataaataaa gactccgcat
    16021 tgaagcatgg ctgtatttaa ataatgaaac ccatgctcct gctccataat ttttccaagg
    16081 tgaggcttaa ccaaatcagc ataatgtatt aacgcaagag ataaaggcag aattaacacg
    16141 ccttgaggca gattcttaat ccattcttgc tcttgggaaa taaatccatt ctgtattaaa
    16201 aacagatttt tgactggtga atcgtcaaag gcggttttaa gagcaggctt tactgtagga
    16261 tcaggtggta caaaatgttg ctgtaatagt gagtcaacga gagtgtactt ccattcctca
    16321 tcatatcggg tgggaaaacc acggcgcttc aaatcagtta atgctttaga ctgcaactgc
    16381 gcaagccagg gtatttgcga tacgcctaat tgggcttgct gttgataata atccaaaata
    16441 tcactcataa ctgctccact tcttcaagcc agctataacc cttcttctcc agttccagcg
    16501 ccaaagattt atcccctgac gtaataatac gaccatttgc caagacatga ataaaatcag
    16561 gctcaatata atccagtaag cgctgataat gggtaactaa aataatagaa cgctcgggag
    16621 agcgcatcgc attaactcct tgagaaataa ttcgtaatgc atcgatatcc aggccggagt
    16681 cggtttcatc aagaatagct agcttgggct ccaaagcggc catttgcaat atttcattac
    16741 gtttcttttc cccacctgaa aatccctcat taatacttcg gtataaaaag ctttcatcca
    16801 tatccagcag ctgacatttt tcccgaataa aacttaaaaa ctctatggca tcaagggtgt
    16861 tttttccttg tcctttgcga accgcattca ctgaggcttt aagaaaattg atattggtta
    16921 caccaggaat ttctaccgga tattgaaaag acataaaaat accggcccgc gctctctctt
    16981 ccggggacat aggcaataaa tcttgtccca aataggatat atcaccacga gttacttcat
    17041 aagaaggatg cccagccaat accttggaga gagtactttt tcctgaacca ttaggtccca
    17101 taatcgcatg aacctctcct ggtttaacct caagattaat accttttaaa atagactgtc
    17161 cattgacagc aacattgagt tctttaatgt ttaacatatt agccgactgc cccttctaag
    17221 cttataccta gtaatttagt ggcttccacc gcaaattcca taggtaattc ttttaaaact
    17281 tgcttacaaa atccattcac gatcatagaa actgcatctt ctgtatcgat accgcgttgc
    17341 tggcaataaa acaattgctc ttcactaatt tttgaagtag tggcctcatg ctctacttgt
    17401 gccgttggat ttttgacttc tatataagga aaagtatgtg ctgaacattg actacccatt
    17461 aacatggagt cgcattgggt ataattacgc gcgttcgtgg ctgttggtgc gatgcgtacc
    17521 aatcctcgat aagcattatg cgcatgcccg gcactgatcc ctttcgaaat aattgttgat
    17581 cgggtatttt tccctaaatg aatcattttc gtacctgtgt cagcttgctg aaaattattg
    17641 gttaaggcaa cagaataaaa ctcgccaaca gaatcatcac cttgcaaaat cacactgggg
    17701 tatttccacg taatggctga acctgtttca atttgtgtcc aggaaatttt cgatttctta
    17761 cctcgacaag ctcctcgttt ggtcacaaaa ttgtatatcc cccctttacc ttctttatca
    17821 ccgggatacc agttttgtac cgttgaatat tttatttgag ccccatctaa cgcaatcaat
    17881 tcaacgactg cggcatgcaa ctggttttca tctcgcatcg gggcagtaca cccttccagg
    17941 taggacacat aactgtcaac atcagcaata ataagtgttc tctcaaattg cccggtagaa
    18001 gcagcattga tcctgaaata ggtcgatagc tccatgggac atcggacacc gttagggaca
    18061 taaacaaatg atccatcact aaacacagca gagttcaaag cagcataaaa attatccctg
    18121 taaggaacaa ctgaccccag atatttctta accaagtccg gatattgatg taccgcttcc
    18181 gataatgggc aaaaaattac tcctttttca gctaatttcg ctttaaaagt agtcgcaact
    18241 gaaacactgt caaacaccgc atcaacggca acacctgcca gcatttcctg ttcgcgtaaa
    18301 ggaataccta atttctcata ggtttttaac aactccggat caacttcatc cagacttttg
    18361 ggagcgtctt ttttactttt aggcgcggaa taataggaaa cggactgata atcaattgga
    18421 ggatagtgca cactcgacca ttcggggtga ggcatagtga gccagtgccg aaatgctttc
    18481 aaccgccact caagcaaaaa ttcaggttcg ccttttattg cagacaaacg acgaatgaca
    18541 tcctcatcca atccgggagg aaaagtttca acttctatgt cggttataaa accatgttga
    18601 tattctctat cgagcagaga atttagttgc tcactgcttt tagccacgat ttactccact
    18661 tgctaattgc cgaactcgct caacatccct tgttggaaga gttggttttg ccaatgcctc
    18721 caaactgaca ctatcaagag cagtttctat cgcatggctt attaatcgcc aatttccttg
    18781 aatggtacat actccctgta aaaaacaatc attgggttgc atactgcatt ctgtgaaacc
    18841 cctgtgctct tccaaagcaa atatgacttg agacaccgaa atttcagctg ctggtctttg
    18901 tagtctatat cctccggata cacctcgtac cgaagttaac aaacccgcgc tagtcaggcg
    18961 ttttagtatt ttactaacag taggtaccgt taaatgcgta tgtaaggcaa tatctcgagc
    19021 attgcataat tcctgcgaat gcttggctag ataaaccatt acaactgttc cataatcggc
    19081 caatttactg acgcgcagca taacaccccc tactcgaata atctagtacc aaataagtct
    19141 tattttcaga tagagtaaaa gaatatctta ttaagaacaa ggattagaca attgcttact
    19201 gatccaatat cacctgatcc tctaagtgag cttttctggt acaatcatcc aaagcaaaat
    19261 tctattacct tattataaat atagtactaa tttggttcta tattcgataa aagagagatt
    19321 ttaccttatt gataaggaaa taagcaaata aaatcttctg ctctatcttg gcgatttcat
    19381 gcgttttaat attttttaca ataggttagt cataaaggga atgggcttgc taatattcca
    19441 tctgtctata atgtgctctg gatttaatat agaatactct tgataaacac aaccaaaccc
    19501 ataagaataa gctattcgat tatttgcttt caggagctag gatgactcac gatactgaac
    19561 taagcgaact gatgcaggcc gtagctaaaa aaagcctgca gattatgacc gatttcaaag
    19621 aaaaacccat cccgctttca tcgctggtca gtcaatacat cgatctcacg gagcatttcc
    19681 aaaatttgat tgccgtcatt ctaaaaaatc ctgaaaaagt atggcaaatg cagttgaatt
    19741 acctcgaaga tgccttaagt ctggctcaag cacaatttaa ttattggctt gaaggaaaac
    19801 cactgcctat taacgatcag cgttttaatg gggaagattg gataaacaat ccttttttta
    19861 acttgctaag ccaacattat cttttggcaa atgagcatat gaattcattg ctggaaaata
    19921 tggaatatgg tgacgagaat ctggcaaaaa gagtacgttt ttttacccgg caatatcttg
    19981 atgctttatc cccagctaac tttattcata cgaatcctca gctcatggca gaaacattac
    20041 aaagccatgg caaaaaccta ttacgtggat tacataattt gctgtccgat gttgaagcag
    20101 gatcctctag attaatcatt aaaatgaccg atactgaagc atttaaaatc ggtgagaatc
    20161 tggctacaac gcctggtaaa gtcatatttc gcaattccat gatggagtta atccaatatt
    20221 gccccagaac aacaaaagta aaatccatac ctttacttat cgttcctccc tggattaata
    20281 aatactacat tttagactta agtccgcata attcattaat acgctggttg gtcgaacaag
    20341 ggattactgt ttttattatc tcctgggtca atcctgacaa aacgtatgcc aataaaagct
    20401 tctatgatta tttaaatgaa ggccccagag aagccatttc agtaattcaa aaacaattgc
    20461 gtgttaaaca agtcaatacg ctgggattct gtataggcgg gacacttctg gcttctcttt
    20521 tagcctacaa taaggcaact aaggatcttt ccattcgcag tgcaaccttt ttagcagcaa
    20581 tgattgattt cagtgatcct ggagacatcg ctgtttttat tgatgagcag cagattaata
    20641 aacttgaaga agaaatgaaa tccaaaggtt atctggctgg caaatttatg gcgtccagct
    20701 ttaattccct aagggcaaat gacctaatat ggtcattttt catcaaaaat tacctgcgcg
    20761 gtaaaaaccc tgttcctttt gacatactct attggaatgc tgattcaact aatatgcctg
    20821 caaccatgca ttctcaatat ttacgttgga tgtatttgca taatgacttg ataaaacctg
    20881 gaaaaattcg ccttaaccat acccctattg acgtaacaaa tatcgacata cccactttct
    20941 ttctgtctac tcaaaaagat catatcgctc cctggaaaac aacatataaa ggatttgaat
    21001 taatgaaggg gccaaaacgt tttgttttag gtggatctgg ccatattgcg ggaattatca
    21061 atccgccaac ccagcaaaaa tatggttata gaaccaatag cagcatggat ttatctgctg
    21121 agcaatggtt tgaaaaatcc aaggaacact ccggttcatg gtggccagaa tggttaaatt
    21181 ggcttaaatt gcattccgga agactcacca attcccctga tatcaatcat ttgccattcg
    21241 cacctataat ggatgcccca gggagttatg tactaaaaaa ataatatttt gaactcaata
    21301 accatacatt aaaacataaa aggagttgta ttgtgagaaa aaaaaacagg atacttttgg
    21361 caagtttatt cttgctaggc agcctgaatg cctttgcctc tcaagatctg acggttgtta
    21421 atacgtccga tgatctggga tatgtgacaa taggatccta ttcctgtgaa ttgacttcca
    21481 gttgtgctat tccccctgat aattcgattc gtattaactc tgatatcgta gaagcagctt
    21541 gtgcgaatac ccccgttgat tgtgaaattg tttttggggt tgacagtata aaagcaagag
    21601 tcgcgaccgg aagctttagc ttgaatgagg ggatacaata tattgcccaa tctcgttggc
    21661 tgaattattc tgctaagcgt ttatccaaaa ccgtggctga aatctataaa agataattcg
    21721 atttattcct tgctgatgtc ctggttgaag cacgcagcaa tagccaggac aatgtttatt
    21781 gaaatgaatt gattaagata aatcataaac acagggatct gattttctcc accagctctc
    21841 tgttcttgcc aaagtcagaa taccccatgg ttgcacttga aataacattc ccatccgcat
    21901 ggatgcataa ccaatcaaca aaatggaaaa agcggctttg tctatacccc accagtaagg
    21961 atgcctcctg cttctgaatt ttcaattccg gaatcttgcg ttcaatgcaa gctaccaatt
    22021 gaggcatact ctcaaccttc aaaggagcaa tatagtgaga gtccgattca ttaaccatgc
    22081 tacttaccca gtttggctta tcatcgggca attgcccctc aactaacccc ttcggcattt
    22141 ctcgaaagtt gatttgagga aataaataaa gaatgacaac aaaggctaaa ataagggcta
    22201 atactatcca tattaatttc atttatactg tttccatggt ttctttctgt tctaaaaatt
    22261 gcttttcaag cgttatataa tcaatttttc ctgaaggcag gacaggaatt tggcatgccg
    22321 gataaatcat atgaggaacc agcaattctg attgccctct ctccttaacc ttttttataa
    22381 atgatacttt atctgcatac tctgcttcag taaataagaa aatttgctcc ccctttcttg
    22441 gagatttttg cacaacagcg gcatgtaata attcaggcca aattgaagct gcaattcctt
    22501 ccaccgctgt aagagacacc atttcaccag caatcttggc aaaacgtttg gcgcgacctg
    22561 ctatagtaat gaatccatcc gcattcacgg tgacgatatc tcccgtatca tgccaaccat
    22621 cttcaggttc tataattact ccaggtttat cctcatcaag ataacctaac atgatattgg
    22681 gtccgcataa ttttaaacgc ccgccttgta caattccctc tactggttct actttgaaat
    22741 ccataaaagg taaaatcatt ccgacactac ccggtactga tgccaacgga caattaagcg
    22801 atatgactgg cgatgcctca gtagcaccat agccttcata aattcttgcc ccaaacgctt
    22861 cgctccagta tctaatggtt tccggtttca ctttctcggc gccagcgaag atatagcgga
    22921 cgctgttgaa atcatgtttg cccgcggccc ttgcataacc cgttaaaaaa gtatctgtgc
    22981 caaacaaaat agtagcccct gtttgataaa ccaatccagg aattacctta taatgaagag
    23041 gggaaggata gaaaaaacaa ttcaaaccat taaccaaagg aagcacactt cctgctgtaa
    23101 ggccaaaaca atggaatata ggtaaggaat taaataaaac atctcgtgga gtaaagtcga
    23161 ctctggaagt catttgccaa caattagcca atatattggc atgactaaga gcaacccctt
    23221 tgggttttcc ctcagaacca gaggtaaaca ggattatgcc tgttgtatca ggggatattt
    23281 tgtcaccaag aaaacggtag cttagagtag gaaagaagcc tttgaatatc ccggaaagct
    23341 tgttacctaa atgaatggtt gatttaaaat cttctaaata atgaattctt atacccgcct
    23401 gttgtaactc ttctaccaag ggttctaatc tggcggtttc tatgaattgt cttgcggtat
    23461 aaattgattt aattcttgct gtattacaag ctgaatataa gttataaaaa cccatgctaa
    23521 aatttaacat ggcaggtatt cgtctataag cttgtaaagc aaaaaaagtg accatgccag
    23581 ctatagtagt cggcatcatt actccaaccg tttcgcctat agccgtttgc tttttaattt
    23641 ggcgccccag aataaaacaa cgagcaagga actgtctgta agttaaggga atacgattat
    23701 tgtcttctat tttagttttg tttttagctc ctatactcac gccttcaatt aaagcagaaa
    23761 ataaaacata tggcttaaaa ctattggcga atgacaaatc acttattagt tgaaataaac
    23821 gtaaactgat accttctctg tcgacacctg tattttcatc ttgaatcaat ttttgagcag
    23881 gtaatatatg aagagtaatt ttcggaaaaa tccgtattcg atgtttattt ttgctattag
    23941 aaaaaatgct gtgctccgcc ccttcaatac gaacagggat tacctcggcg ccaacctttt
    24001 ggagtatcaa tccaggtcct tcaaagacct ttaagctgtc ttcctgctgt cttaatagcc
    24061 cttgtggaaa tagaagacaa cgcttgccag cccgtattgc ccttattaaa actcttgttg
    24121 cctgagcgct gcctggatca actactatga catcagcaaa aaataataac gtttttaccc
    24181 atattttttt aaacatatca ggatgtaaag ctaaagttaa tcgttcgggt aaaaatacag
    24241 aaaggagtaa cacatcaatt atagaagccc tgttggcaat aatgacagaa tggctgttgg
    24301 cctgatattg tcctttaacc tctatgcgaa accatttctc cagaagaatt tttatccatg
    24361 ccctagcccg ctgtttttgt ttttccttcc aggtcaaatt ttcctcattt gtttttgtca
    24421 tcatgtcgca tcagttatct ctatattgac ttttattctg caagattcaa aacgatttga
    24481 atagataggt ttatgaatca tgttgatttc atttaaaaaa tttaagcgaa agcaacatct
    24541 gtttttatga taatttaccc ggaatattga gagaaggctt gcgagtcctt aattcctgta
    24601 ataaagtgag gggcatcttt actttaacac aaaaaaccat taagacattc tgtttaattt
    24661 tagccatatt agttttaatt attttcgcag tggtctttca taatcattcg ttagagatca
    24721 ttgattatat agacgatctg ggttggcttg cccctgttct tttcttgatc acctactgcc
    24781 ttgcgacttt attgctttta cccacaatgg tattaactct ggctggggga gctgtgtttg
    24841 ggcctgtatt tggcaccttg cttaatttat tgggagccac tagcggcgct gcgtttgcat
    24901 ttctgataac aagacacctg gtttacgatt ggttctcaac caaaaagggt gaaaaactaa
    24961 acaaattgat tgccggggta gatgagaaag gttgggtttt tgtggctttc ttgcgattgt
    25021 ttccaatagt cccttttaac ctggtcaact atggcttagg cgtaacgggc atatcatttc
    25081 gtctttattt gctaactact tttatttttc taattcctgc agaaattatt tacacctatt
    25141 ttggatacgt cggcatggat gctctgacaa ggccaggcca tttatatagg aatggtggaa
    25201 tttttctaac tggattagcg attttctttt tacttgttat cagaataatg aaaaaaagat
    25261 aattattttt tcaattaata aaaacttatc aagactcatc ctgatttaat aaaaagatca
    25321 acttatcgat aatcggatga ttgacttcaa aaataatgtt atccagggag agcacccttc
    25381 tgataccctg cagactatta gttaaaaaaa cagcatcggc gtcctcaatc ctctttttcg
    25441 ttaatgatat ttcctgcact gacattttgt gttgctgaca atgtgaaatg aggcgtgccc
    25501 tggtaatgcc gggtaagatc ccatcatcta ctcgcggggt atacaaaatg ttattttcaa
    25561 tcaagaataa attggcacaa gttgtttcag taacatgatt ctctgtgttg aaaaataagg
    25621 catcgtctgc cccaaccgca atcgcttgac gttgagcaat aatcgcctct aaataattca
    25681 ctgattttaa ttgataaaga ggattggcct tatcccttag ccaattaata ctgattaacc
    25741 gaacaggatg cttttgtatt gaataattaa aagtctgaaa tattaattgg ctgacttgtc
    25801 ctcgttctgc caagcctctc gaagcagggc caccactcaa aatggcttta atgccgccat
    25861 ggtacaggtt atctttttgg attttttgaa taagatgctc aaaccaatca tcaaatgaaa
    25921 tttcaaaagg aatacccaac tgacgagctg aattgcctag tctctcccaa tgcatataag
    25981 caaaggaagg cttggaacta ttgactctaa ttgtttcaaa taatccctca cctaaaaaaa
    26041 ttcgatcatc aattccaaat gatggcgtca tatctccttt gtcttcaata acacgagtag
    26101 gcattatgat ctcataatta ataatctaca aaatcatcca actcatctcg caatcgcttt
    26161 tcctccaaca tattttccag tcgtctgcgc gcatccaaac tcacaccggg agcttctata
    26221 atttcagtat ccacttcaac atcatcaaaa tcgtcgacaa cttcaattac ttcttcttca
    26281 tcttcttcaa acaagtcact cataacattc cctcaaagaa ttaaaagtaa cattaaataa
    26341 ttgcttaaat ggaacaagat tccaggacgg ctatatacct tatttttaat tttttgcata
    26401 gtatttttta attattttat gttataaact tatcgcatcg cgcatttata atcacccaat
    26461 gcataaaaca acatattgat acctggaaac acttgaactt taagctcaat ccatctaata
    26521 ataaaaaaga atattagtga taccaaatca tgtcagacca atataaagtt gctctaagaa
    26581 ataccataaa acaggtaaga tccaagcttt ctgtatccta cagaacagca tcttccaatc
    26641 aaatttgtac tcgtatccgc tcgctggaac aatacaggca agcaaagcga atagccttgt
    26701 actttgcagt taatggagaa attgatttaa catttctctg gaaaagcgcg cccctccagg
    26761 ggaagtattg ttattttccg gtattaaata atgattccac tctttctttt ttaccagcca
    26821 cccccagcac cccttttaaa acgaataaat acgatatccc ggaaccggac gttgatattg
    26881 agttggcctt gcctgttgat gaattagact tgattatcat gcctctggtt gcatttgatg
    26941 cacactgtac tcgtttgggt atgggctcag gctactatga tcgcaccttg gctaataaat
    27001 caaaagcgca tctcttagga gtcgcctacc aatttcaaag ggttgattac atagaaccac
    27061 gcccttggga tgttcccctg gatgccgtaa taactcaaaa agctatttat tggcgaaatc
    27121 ccttggccta aaatgacatt ctatagagcg tctgtaccga cctccccggt tctaaccctc
    27181 actacctgtt ccaaagcata aacaaaaatc ttgccatcac caattttacc ggtgtaggcg
    27241 gacttgcaaa tagcttctat ggctgcttca accatattat ctggcaatgc cagctcaatt
    27301 ttaattttag ggaggaaatc taccacgtac tccgcacctc gatataattc ggtatgacct
    27361 ttttgacgcc caaatcctcg agtttcagat atggtaattc ctggcactcc tatttccatt
    27421 aaagcttcat gtacatcatc cagtttaaat ggtttgataa tcgcagtgat cattttcata
    27481 tttttttcca tcacgttaaa tgcagagcca cccatgatat ttctaacaca caatttttat
    27541 gttattgctt ttcaagacat cttgggtata gttgtaatcc aacagttttg ctagactgta
    27601 ttcaaaaata atccggagcc accatgtttg accccaagca atttgatgaa ttagctaata
    27661 agcttttcgc tactttacct acgagtctac aaaattttga aaaagacatt caacaaaaat
    27721 ttaaagaagt actgcaatca acattcagcc gaatggacct tgttacccgt gaagaatttg
    27781 atgtgcaatg taaagtactt gccagaactc gagaaaaact ggagcaattg cagcatcagc
    27841 ttgaagactt cattaaacag caagaaatta aaaataataa ataataacta ttagtcgtcc
    27901 ataggaaatt ggatcgattt aagtccaaca taataaggaa aaaatcttat gagtctcgca
    27961 tttaccaaaa cgcgaagtac tattggtatt gtcgcacaac cagtatctgt ggaagtccat
    28021 ttatcaaatg gattgccaag ttttactatg gtaggacttg ctgaaacagc agtgaaagaa
    28081 agtaaagacc gcgtgcgcag cgccattata aatagccaat tcgaatttcc atgccgtaaa
    28141 attaccgtta atcttggccc tgccaattta cctaaaacgg gaagtggctt tgacttgcct
    28201 attgcactag ggatactcgc agcatcagaa caaattcctc tcaccaacct tgcaaaccac
    28261 gaatttattg gagaattggc tttaagcggc gagcttagag gagtttctgc cattataccg
    28321 gctgttcttg ccgctcataa agacaatcaa catctgatta tagcgaatgc caatgctgcc
    28381 gaagcttctt taaccggcca tcaaaaagtc tttactgcca ataacttacg tgaagtttgt
    28441 gattatctat gccagggcac ttctttacaa agcttacctc ccaaaccaga tcttttatta
    28501 aataactatg aattggattg gtctgatatt aagggacaac agcatgccaa aaatgctatg
    28561 gtcatcgctg cctgtggggg acacagcatt ctgctaagtg gcgcaccagg aagtggaaaa
    28621 acgatgatgg caaaaagatt tagcaccttg ctacctgagc tatccgaaac acaagctctc
    28681 gaatgcgcag ccattaattc aattcgaggc aaattacctg atttcaggga atggcgtttg
    28741 cctccttttc gtgcaccaca tcatactgct tcacctgtcg cgttagtcgg aggaggcaac
    28801 ccgccgaaac ctggtgaaat ttcattagcc catcacggcg tattattttt agatgaatta
    28861 cctgaattta atcggcaagt attggagaca cttagagaac ctttggagtc agggcatatt
    28921 tgcatttcca gggcagccgc acaaatagag tttcctgcca aatttcaatt aattgcagca
    28981 atgaatcctt gcccttgtgg ccaatggggt aattcacaag ccaattgcat gtgtactcct
    29041 gatagaatca gccgctattt ggcaaaactt tctgctccat tattagatag aatcgatatg
    29101 caggttacaa ttcacgcatt atctcaagag gagttaatta aacccaatac ccatttagaa
    29161 aaacaaagtc tcgctatcag agaaaaggta acaaagatgc atgaaataca aatggcacga
    29221 caagatagtc tcaatgccaa tttaaattcc aaaacatgtg aaatggtttg cgaattggga
    29281 tcagaagaac aattgtttct ccgagaagtg atgagtaaac tgaaactgtc tgctcgtggc
    29341 taccatcgct tactaaaagt aagcaggaca attgctgata tgaatagcag taagaaagtt
    29401 ctgttaaatc acttgcaaca agctttatct tacaaacaaa atttacactt accgaaatga
    29461 cactgattat aaagataacc cgtacagtat tgatgattaa gatatgagca catatttttt
    29521 aaatattgca ttatacttaa cttacacaga ataaattaag gtattaatat tatgatatta
    29581 cgcttaatgc ttaccctctg ttgtattgta ttcccttgca aacttttaat ggcgaaacaa
    29641 attatggata atgccattat gatagtaaaa aattgttatg ataaatccac tgatcttagc
    29701 aaaacttttg taccatgtat taatgaagaa atagaaaaat tacccgattc taaaaatttc
    29761 caaatacgca tgaaattgaa aagtgctgaa aaagataata atgaaaaaat atctattctc
    29821 atggtcgata aaacaggata tatgtattac tgcatagtaa ccacaggaaa aactttaatc
    29881 ataaatgcct gtgccggaga acagggaaaa ccattgacgg aaggtcaaat gttatccatc
    29941 gatctacctg aataattttg cggtttccta tctcttgctc agcgtggtta atcatgcgtc
    30001 ttgcagtatg agtagaatat attacccaac agaataaaag cataatgcat gacagaatat
    30061 agagaaatct aactcgctat cagcgattca aggatagcca ttcttgcata aacaccgtta
    30121 gtaacctgtt gtaaaataca agattggttg ccatcagcca cttcactatc aatttcaacc
    30181 ccgcgattca taggcccagg gtgcataacc atagcgtcag gttttgcata agacagactt
    30241 ttttgagtaa gtgcaaaatt gtttctataa aaatccaaat ccaaatgatc atcttccagc
    30301 aagcgttccc tttgaactcg caggcaaatt accacatccg caccttccag accttcattg
    30361 agattatcag taacccgtcc aaagtgcact tgtgatggtt gccaaatctc tggagaaatt
    30421 aaaaccaagt caccaacccc tagtttactg caaatgcatt ggaaagaatt agcaacacgt
    30481 gaatgtttaa tatttcccaa aatagcaatt tttaatttat ctaatcgctt tttttgttca
    30541 ataattgtca ccatatccag tattgcttgg cttggatgag catgagttcc atcaccagca
    30601 tttattatat gaactgtatc acctaactta ttagctaaat tttgctgcag cccatcttgc
    30661 ttatggcgaa ttacaaaata ctggataccc atagctgcta gcgttctgat ggtatcttca
    30721 atggcttcgc ctttaatttc tgatgaggtc tgaagatcaa gatttactac agacattgcc
    30781 agatggcgct ctgctaactc aaagctgatg cgagttcggg tactgttttc ataaaataaa
    30841 ttagcaataa tactttggga ataagaaggg tattgctttg tatgtttaaa atataaagct
    30901 cgttgcaata aggattcaat ttgttcagat gacaattgac taatttcaag aaaatgtttc
    30961 atgttccccc gccaaatttc attttgcatc aaaagaaaag tactctttga gttattccga
    31021 ttgttgcagc cactgctcca gtataacaca agctgctata ctatctacct cggctttttt
    31081 aatctggcga tatcccccct gctcaaacaa ataccctctt gcctctactg ttgaaagtcg
    31141 ttcatcgact aaatgcacag gtaaagaaaa ccgcttgtgc aattgctttg caaaatgacg
    31201 agcagctgat gttgtataca attcacggtc atcaatacag gtaggaagac ctacaattaa
    31261 agcttgtgga ttccattggg ttatcacttt ttgaatggta ttccaatcag ggattccagc
    31321 tttagcttct atagtagata agggagaggc actacaagtt agccgctgtc ctacagcgac
    31381 tcctatgcgt ttatacccga aatcaaaacc taaatacaca cctctaggca tggccagcat
    31441 cagaagagag ttgattcatt ttgatgccca gcgttaatcc cgcgtattcc caacgctctt
    31501 caaagggcac ttcataaaga atttcagatt tatagggaca aactaaccac gtgttactca
    31561 taatttctct ttccaattgc tgctctgtcc aagcagcata acctaaagtg attaatacat
    31621 ctttaggtcc ttcgtcataa gctatggcac gaatgatatc atttgaggta gtaaccgtca
    31681 cttcatcttg caaaaacaaa ctcgaacgcc acccgcccat ttgtttatga ataacaaatc
    31741 ctctttctgg ttgtacgggt ccaccaaaca ataaaggcaa cccatttttt tctactcgaa
    31801 taggttcaat ttgcaattgc tcaaaaacga tagatagtgg aaactgtaaa ggtctgttaa
    31861 taattaaacc aactgacccc tgctcattat gctcgcacaa atagaccact gacctttcaa
    31921 agtttggatc ttttaaggaa ggcatggcaa tcaataggtg attggccagt gaactgataa
    31981 ttgccataag aagtcccccc aaaattctct ccgctcactt taattataca ccattaatga
    32041 ttttaatttt atcgttttgt ttttaattta atctatttgt ggtggcatga gcccacaata
    32101 ccgaattatt atgattttca tggcttaaag atgcttacga gaaactccag gatcaaggca
    32161 aaatattttt aatgaaggga ttaggagtta gttaatcagt aaatcctggc ttattctcca
    32221 gttcagggaa gaaaagctca ttaagagaag atccaagtaa aatgattatg aaacgcattt
    32281 ccaccatttc atttggtggt cccagaaggg gtggatttta caactgatac cttaatgtac
    32341 aaagttgaat tttgagaaaa aggccttcca gctgtataag aagttgaaga agaatcggta
    32401 ttttgaaccc cctctgctgt acctaatgga acccatttat tcaatggcgc catgatcgta
    32461 gtatctattt gttgattatc aaattgttga tcactctcta cattttcctg ctctcgtact
    32521 cttctcactg tcaatttgac ctgtgatcct tgcaaaacag gatgtactag caaaccattt
    32581 tggatattat gctgctcata agatactcct gtggctaaaa gaccaacacc aactgaagta
    32641 actactggca cttgttcacc ggttgcaaca aaagcggatt ccccactcat gactttaacg
    32701 gattgactgc gctgtacttc atattgtggt tgtgtgctgt aagagacatc attcccatcc
    32761 tgtgagctta accattcagg atccccttga taaacagtaa tattaaaaac aacaggcgga
    32821 acatctattt tatgtaatac gtttctgatt tgcgttaaag tttggggact cacattaaca
    32881 attagtgttt gccctgaacc acttactttc tcatcagtct gcaataatgg ttggattaac
    32941 tgaatgacct tatctgcttg caaataattc aattcgataa ccttggttat catctgttgg
    33001 gaaaaagcgt tgccagaaat taataatatc actaatagcc atcgcttaat catagaaaca
    33061 ctcctgttgt ttcaatttaa ttataaaatc ctttattgaa ttgtagcact aatagttgga
    33121 ctcacaaggt tacaaatagc cccctttaat ctctaatctg tattcactgt agataagtag
    33181 tagttttgtt cctcaaaaag tttaggtata atccttaaaa tctgttatcc gagatcaaaa
    33241 attatgggta gtatattcaa tatgttcggg ccatcaccaa taaagcccat tgaacaacac
    33301 atacgtaaag cacatcagtg tgccaagcaa ttgtaccctt tttttgaagc ggtgctcaaa
    33361 aatgattggg atactgcaaa caagatcaag gataaaatca tcgctattga aaaagaagcg
    33421 gatctaatta aacgtgattt gcgccttcat ctacctactg gcttattttt accagtcgcc
    33481 cgcacagaca tactagaact gctcagtgct caagatagaa ttgccaataa agcagaagat
    33541 atagctgctt taattataag cagacaaatg agcataccta aaaaattaat cccgtctttt
    33601 atgccttttt taagccgatg tcttgatgcc tctaaacaag cctgcacagc gattaatgaa
    33661 ttagatgaat tgcttgaaac cggattcaga ggcagcgaag tcaaaatcgt tgaagaaatg
    33721 atagtaaaat tagacgaaat tgaacatgac tgtgatgaaa gactggcaga cattaggcac
    33781 aaaatcttcg aattagaaaa ggaactccca gctatagatg taatttttct atacaaactg
    33841 gtccaatgga ttggcgaatt ggctgaccat gcccagacag ttggtggtcg acttcaaatt
    33901 ctaattgccc ggtagttatc actcatggat tattcaattt acctactttt tgctgctctt
    33961 attctttgtt ttcttatgac ctggggggtc ggcgccaatg atttggctaa tgtcatgagt
    34021 accactatgg gctcaaaggc agttaccgtc cgtcaagcca tgcttatcgc cattattttc
    34081 gagtttgcag gtgctttttt agggggtgaa ggtgtcactg aaaccatgcg tgatgggatt
    34141 atcaatacca gtcaactttc taatgaacct ttaatcctca tcgaaggcat gctgtgcgtt
    34201 ttgtttgcct gcacaatatg gatgaatctt gcgagttatc tgggggtccc tgtttcgata
    34261 accaacgcat tagtaggctc catggttggt tttggcacta tagtcttggg tcctgacgca
    34321 atacattgga atcaggtagc gcgaatcgct attggatggg tttcatctcc gttgatttca
    34381 gggattacgg cctacgcttt gttcattagt atacagcaaa ccatatttgt aaaaagtaac
    34441 ccgttgacta aagccaagct atatattcct atttatcttt ttttgattgg ttttatatta
    34501 tcttttatca ctgtatttaa aggacttaac cattttgata ttcacttgaa tttaaagcaa
    34561 gatttggcgg tgactctggc caccagtata ataattacta ttctgggtat gatctttatt
    34621 aagcgtattc ctgaatacca taaaataaga cgcagggaac gatttattca ggtagagaag
    34681 tattttgccg tgctcatggc aatgacagca tgcgctatgg ctttcgctca tggctccaac
    34741 gatgttgccc tggcagttgg accattaagt attgtgcaca gtctggttat gcattccaat
    34801 caaatatttg acgcagataa ttacccttcc tggattattt tattaggctg cgtcggagta
    34861 gtaactggtt tccttatgta tggaagaaaa gtcattgaaa ccgtaggaag ctctatcact
    34921 gctttaactc ccagccgtgc atttgctgca acactttctg cagcaactac cgtagttgtt
    34981 gcaaccagca ccggaatccc tgtttcggcg actcaaactc tggttggtgc cgttttaggt
    35041 gtaggtttgg caagaggtat cggcgctttg aatctcattg taatccgtaa tatctttatg
    35101 tcatgggtac tgacattacc tgcagcctcc atgctaacga tcttatccta taaactttta
    35161 catgctttgt tgggataatc ctcattgcct atcaggctca tcataggcaa tggattgcct
    35221 aatattcaac gcctactgta atatgaaaat gaattgaata tcctttaaaa tataaatttt
    35281 caggcaaacc atatgctttt ctcaatctta ccaaagagga tgcctccact ataatggcat
    35341 aatatttttt taattctaaa tttgctttaa taatattctt aattttaaag cgatgctctt
    35401 gatctaactc ttttggatta attgttgtat tttcttccgg atagataata ctaatatgag
    35461 caccgatgga ttcattacca aaataatcag gtttacggat ctgctcgttt tgtaagagcg
    35521 gaaatagttc gtggatccat tcatcatgaa tcttaagata agttaattga ttttgagaaa
    35581 tggctacttt cccagttgat ttaagctgca tagccttatt taataaagca ggattattta
    35641 gttgcagaat ggatggattt ttcatcttta tctttactaa tacaataaag acctgtcatt
    35701 tctgctacta ggtcttgatt aacatgctag cagcttacca agatagctat agaggagaaa
    35761 ctcttaaaac ccttcccaaa tttcactttg atcgatcttg tagtctttat cgtctctcat
    35821 tttgaagatg cgctcatgtt ttttaatttc atgtaactgc gacgctgact ttttatgtgt
    35881 cgcatcaaat tcatataaaa acttatcgta aggactgaca taagctttat ttatatcatc
    35941 tgtgttcata ctcaatccaa aataacttta tttaaaaaat atattttatc gtttttttat
    36001 ctaagttacc attaatcttc tttcaagtaa gataataggc tatatcatga atttaaaaaa
    36061 ggcagaatta cacgtacatc tagaaggaac tatttcccca gacctagcca aaaaactagc
    36121 aaaaagaaac catttaactc tccctgacgg tcttattgct cttgatgaga aaagttactt
    36181 atcaaaagac tttctcgatt ttcttaaggt ttatgatacg ctggcatccg taattaaaaa
    36241 tccacaagat tattatgata ttacctttga ttatcttaaa tcaaacgctc aagaacatgc
    36301 tatctacatt gaaatgatgt actctccgga ccatgccgag caatccagtg gaattccttc
    36361 aaaagagcat ttagcagcaa ttcaacaggc tattgatgat gctaaaagtc aatttgatat
    36421 tgttggacgt attattatca ctgcagtacg ccattttggc gcagaagcat gcgaacgcgt
    36481 cgcaaaacaa gcttctatag ataaatttcc ttgtgtaaca ggatttggat taggaggaga
    36541 tgaggcaaaa tttcctcctc aattgttttc caaagcttat cagatagcag cagactccgg
    36601 attggaatgc accgttcatg ccggcgaatt tgattcagcc aaaggaatgg aagaagcaat
    36661 gaaaactctg ccaattaaaa gaattggaca tggggttcga gtgattgact cacctgacat
    36721 catggccatg gttaaagacc agggtattgc attggaagtg tgcccaacca gtaacatatt
    36781 tttgggatta ttcaaaaaca tgaatagcca cccttttcca aagctttatg aggcagggat
    36841 taaagtcagt attaattccg atgatcctcc ttttatgagc actacacttg ctcaagagta
    36901 caaaagagtt caaaaagctt atggctatag cgataacaca atgaataata ttacgcgtat
    36961 ggctattgaa gctgcttttg ttgatgaaaa aacccggaag gaacttttaa caaaattgga
    37021 acaataatct cagtcttata ttcctgttgc cagtactaat ttcatgctgg tttgaagcta
    37081 aaacgatact gttatatttt tacaggttgg tgttgatata tggcttaaac accaaccata
    37141 actaaaacca tctggttgaa attaaaaaat attttggttt tttataaaat tcagtttaat
    37201 cccttggtag accaagtacg acctgctact attattttat tttcctatta attgactatg
    37261 aaaacaaaac attttgacct tatagttctt ggaggaggaa gtggcggaat agccagtgct
    37321 gttcgtgctg ctcagtatgg agccaaagtg gcggttattg agcaaaacca cttgggaggg
    37381 acctgcgtga atttaggatg tgtccccaaa aaaattatgt acaatgcttc atcaattgca
    37441 gaaacactcc ataaatctcc tgattacggt tttttcctgg aaaataatgc caagttagat
    37501 tggaagcgac tagtgaataa acgcaatgcg tatattgaac gtttaagaga aaattatgcg
    37561 aaacgatttt cacagcacaa aattactctg attcaaggta agggtatttt ccacgatcaa
    37621 agttctatca cgatagatca tactatttat caagcagaac atattattat tgcaactggc
    37681 ggtgagccag ctctaccagc aattaatggc attaagcatg ctattgattc tgatggtttt
    37741 ttttcattaa ccaaattacc agctaaggta gctgttattg gtagcggtta cataggtgtt
    37801 gaattagcag gaatccttaa ctctcttggt agtgaaactc acttgttaat gagaggtaca
    37861 cgaccactga gtcgctttga tcatatgatt ggcgatactt taatggaaat tatgcaaaaa
    37921 caaggaatct gtattcatca aaaccataaa gcccaagcta tccatctcca tagcgacggc
    37981 agaaaaagta ttttgtgtca aagtggctct attattgaaa atattgatgt cattatatct
    38041 gctgtaggtc gaaaaccgcg gactggcaat ttaaatcttg ataaaataaa cgttaacatg
    38101 gatgacaaag gattaatcct ggttgatgca ttccaaaata catcggttaa aggtatttat
    38161 gctattggcg atgtcactaa tgctcccgca ctaactcccg ttgcaatagc ggcagggagg
    38221 cgtttggcag accgaatttt cgggaatcaa cctgatgcct gcttaaatta tgataatatt
    38281 tgttcagtag tattctctca tcctccatct ggttctgtcg gtttaaccga gcatgaagcc
    38341 attgaaaaat atggtaagag taaaattaaa atatatcaaa cccgtttcat tcctatgtat
    38401 gatgccttga gtgttgataa aacccctact gcgatgaaat tggttacctt gggtaaaaag
    38461 gaaaagatta ttggtttaca tgtcgtcgga tacagcgccg atgaaatgct acaaggcttt
    38521 ggggttgcca taaaaatggg cgcatgtaaa aaggacttcg ataatacaat tgcaattcat
    38581 ccaaccagtg ctgaagaatt tgtaactatg gtgtaaatat acatgaacta ttccactcgt
    38641 aaatttgaag acaagtatcc tgaattaggt gaacgagttt atattgatcc gcaatccact
    38701 gtcattggta acgtaaccct tggagatgac gtatccgtat ggcctatggc tgttatccgt
    38761 ggtgatgtta attacattca aataggccat tcctgcagta ttcaagacgg tgctgttctg
    38821 catgttactc atgatggtcc ttatactcca ggcggtaggc cattaatttt agggcaagga
    38881 atcacggtag ggcacaaagc tctattacac gcctgtacta tcgatgatta ctgtttaatt
    38941 ggcatgggat ctataatttt agactcagcg cacatacaga aacatgctat gattgctgca
    39001 ggaagcatcg tacctccggg aaaaatactt aaaagtgggc atttgtattt gggaagccct
    39061 gctcaagccg tcagaaaatt aaccacaaaa gagatagagc aaattgagta ttctgcaggg
    39121 cattatataa ggctaaagaa taaatatctt acatattagg ttactagcta ctcatttttc
    39181 ttatccacat gttacttttg ataaattaaa aaaggaaatc aagccattta aactcaactt
    39241 atgacttaaa gagccagtta agtaacgagt attttatgta gaaaagacgg tgtaatttat
    39301 tttataaaaa aaaagcggct tgtgagccgc tataaggaat aaaaccctgg cgatgaccta
    39361 ctttcgcatg aggaagcctc acactatcat tggcgcggaa atgtttcact tctgagttcg
    39421 agatggtatc aggtggttcc aaatcgctat agtcgccagg aaaactggtt tacctgaggc
    39481 tttgattgct gt
//
LOCUS       DAXLDT010000011        39151 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXLDT010000011 DAXLDT010000000
VERSION     DAXLDT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 39151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39151
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.011"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1656
                     /gene="lem3"
                     /locus_tag="ACF2SP_000476"
     CDS             <1..1656
                     /gene="lem3"
                     /locus_tag="ACF2SP_000476"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector phosphocholine hydrolase Lem3"
                     /protein_id="HFD2358283.1"
                     /translation="RDKIITGKKIIFSQSVAKDQTKDLSNFLAERFYSVNQSHNHAII
                     IGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNGARVATTDGLGGGNGDEEEDDEIY
                     KVSHFSCENFLNCDQNIDTTLNLITQPKASEKKQTAPKTLQHTEASMAAFIYQNHPGK
                     GYIGEFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTWTPPSLQALATTANKDALLV
                     RQTLKLAEGDIIISMTDGIWGELKTSLIAQTNDRRDIGVDKEYFKTLFDELTDSPYPS
                     SFDIARIITSSAMSRSLERRKTLIKLINEIEQQHFHEKSVKTINEVLEYFLKTGHVET
                     AQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTVGDCSTINVTRIPYHLDELI
                     RSFINYPEKHQILAPLFKARIKSEADLEEAFHRLSLETVQAEIECPISEAHFERAFKK
                     ETLDKTQAVLTHYFRISTGLDSKKNYQERLNDLSAYFSKESSLEKNDIKLLLSMLDSE
                     IKPKTGVFQTLFGENQNKLYKAFHKKIELQLLDGEIENKNELK"
     gene            complement(1663..4512)
                     /gene="ankN"
                     /locus_tag="ACF2SP_000477"
     CDS             complement(1663..4512)
                     /gene="ankN"
                     /locus_tag="ACF2SP_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkX"
                     /protein_id="HFD2358284.1"
                     /translation="MVRTMPNLPGLYFFQAYPREEIWRLFVDGRFWSKENGWRGYESR
                     EPGCLNAALESLCSIALQVEKSGEEFELSVDLIKKIHKKCGKKVEELQEKNPGELRTN
                     EPVSFGIPAGRASIKGIEEFLSLVFLTEGEAAFGPGKAGPFGPCFDKNYFKNLSPEQI
                     PNLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSFNQEIKIAETLDEKLKII
                     VKHIRMYEVLHPFRDANGRTFVNNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVV
                     KEAIFNTVEIIEQSKKKTPITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDLSDLNPE
                     KLHRKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRDESQINQRIEQGAPPLYVG
                     KTPAHLAVISGNMAMIDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLS
                     QEDAIKVLNIKDNHGKTAFHYAAEFGTPEIISALTTTAVIQINEPDNSGSSAITLAYK
                     NHKLKIFDELLNSGADISDELLEAIWARKDKETLGKIIAKNEKILLNKEAFRIAISLG
                     SVSLVKKFLRAGVDIDIPLTKEQATPLMLSVNSGHLKLVSYLLKKGANKSTTDTSGNS
                     ILHYVFYSKAENREALVNIITENDKKLINQPNANGNPPLYNAVVVNDLKMATILLEKG
                     ARVDFEDRLGNNILHSVMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHHALHEMPTF
                     PSSKETEEIHFMNLSDFLLKEGVDLNKKDIKGKTILDIALSKQYFHLCVKLMKAGAHT
                     NISSSSKFLKNSDANSILERPFKFKNDLKKELDENPLIAMAQINDLYVQIKNNRIRTP
                     TGYAPKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKF
                     FDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW"
     gene            4674..6383
                     /locus_tag="ACF2SP_000478"
     CDS             4674..6383
                     /locus_tag="ACF2SP_000478"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFD2358285.1"
                     /translation="MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMP
                     LGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQR
                     EYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLSLESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGE
                     DLIFYSDASDYAANIEQATSLKPPKANQACNETITLVDTPNQKTIDEVASFLGIASNQ
                     TIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFIDEELILKTLKTPLGS
                     IGPIQLNIPVIVDHHALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDQ
                     SPDGRGTLHCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVA
                     AAIEQHHDEHGIIWPQALAPFQVNIIPLNGARSQTVKEQAESLYQQLKSHGIDVLLDD
                     RNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVVNFIIEL
                     INK"
     gene            complement(6741..11024)
                     /locus_tag="ACF2SP_000479"
     CDS             complement(6741..11024)
                     /locus_tag="ACF2SP_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358286.1"
                     /translation="MKITRNQLNFKGVKEFGGCLTRNDEQIEFINKEIKLLKELIEFN
                     ILKGIDENKSQSLFDAVIEAVEKAGLDVEHELLIQFIQSTSAIIDSKSTDLPELISLL
                     TVLIKSSKKYDKSELTRYCRLIDKLEKTDEKSLEQWIKILTILGRNVESVNDDLLFNV
                     QSRLEIHSVLLKDISSLFDYPPYPALDVFSEKLREYSRELKLYIDSFDRDPAGSRAPK
                     VNQEGILVKSREQILNEQFDTSQIQEVIAKIESLVDGSGLSCKGQYELAQQVVFINAI
                     GRDFPLKIANETYTDLTQLGRNKLQELSDFLIMEMRRLDISKEDKLRTQLKLLAVMRE
                     QYFRSTGIFFDTTQMISLLLSLHHQEQNVLMGVSCNENLSAPPAAILAAMQWVLADGG
                     TVDLCFANHGLIAQDYLEKGDRDFFTCLGIPSSVVEANSESGTYRVGGINYSTLSDLT
                     SYHSHAVIEDEELSMDRCGYPVSSYLILNHIDFSNVDDRTLSHFSSLYDYYKGKGRVI
                     RITEIEETVGEVVGQISRFDMDVGYRVFPARKNLQEELNTQSLAERRREEAVKQYFIQ
                     SVSNIQQAVLNQLEEWQAFLHLIYPKSEWRQLDNELFAQRNELIDSLKKQWTKCLEDS
                     DPKKMYPNPYVRRDVFQRLQTRELEKAIQAYEKSVSSIWNLNRDQLKAKTENRFASDS
                     VNALRCKYLEEVDLSEQIKWSKLSARLKKKEATHEKKRSHRHLESAVEVTGAMLSYHD
                     GDLEPYRLAFVHSQLKLWARDISKEINNSSLPACVKQGLIERANNADNFLALELLLID
                     YESRWLDKEKLSEKFRMQPIISDMLRVYKQAGFEEHKELKILKETYLDSTATQIIHHL
                     DKTLAWAKPENRGPWYWIERSAVKKAANEILVLANGVKQARDSLSKQIAIKKLYRGLC
                     KHQAQLEDVWIFSFDHKNTRDLINQTLNTMDSLTVLGRDEGGLDINFIQECKEEAQYY
                     LLGEKFSSVLEDFEKNNSQILTAHSEWQKVKRQLQQKQNESRKVYTLNEMYYVLCKKI
                     NELSISYSLLREPLVQLRGMIRTLWNSFQEKHQDIIDESKYFDFKARKIKEELEGIEG
                     YSVFNVKVKKGYTGFNEYVELIIEGEGTSALFEGFMQYKSRLTELKEEYRQLEFSLKQ
                     NRLELLNLNRIHAKHLPFLKDRIKGNPEVELFPEQCRSKVKEILTLKEWINGQIPENL
                     SQFPENMQNHFLDRNTLKNMDRNTMTMEQIDKIKDEQLKADLTELYNKMHKVDEEQSQ
                     SWFSTVTRRIVGLIPSMFRQEETEDDWRYQFNELQGRTDSILQSLLTKKIMTKYDALV
                     NEMVLIQSNLAQQMDSGNKKIQFLSDKILEEERKTNVIIKRFDNLNDFYDFEAKLKRV
                     KTAPPIAPVNEVQIESKNRILSCGEGAFSNPEEEFQTDSVMQPPMVFSI"
     gene            complement(11113..12921)
                     /locus_tag="ACF2SP_000480"
     CDS             complement(11113..12921)
                     /locus_tag="ACF2SP_000480"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFD2358287.1"
                     /translation="MQNTAKINQLSVAFKSPDKTVLALDKLSFSLNPGETLVLLGESG
                     CGKSLTSLAMMRLLPRAGVYGIDSQINIHGEDILNLPEKMMRELRGRRLAMIFQEPMT
                     ALNPVLTIGEQIAEVLKRHRSLTKEQIKERVILLLNEVEMPQPQMRIYQYPHQLSGGQ
                     KQRVVIAMALACEPDILIADEPTTALDVTIQAQILALLKKIQKKHQMSLLLITHDLGV
                     AKTMADRVCVMYAGQVVEQASVKDFFSKVSHPYVQQLLTSLPSFAKRNEKLSIIAGTV
                     PSLESMPSGCRFHTRCIYAFERCHHEEPQLQEVEQRILRCHLYPDIKELPLLDKNKIS
                     WVPSVEKEEIVFSVDDLSVHFIHKKGFLSRKKTIFKAVDGLSFTLSRGKTLALVGESG
                     CGKTTASRALLRLLPAAGGEIRYKDSDVLSMTGSALRRYRKKVQIIFQDPFSSMNPRM
                     TVGEIIAEGMHAQGMKPSYIRKQQKSLIEQVNLPITSLHRYPHQFSGGQRQRICIARA
                     LATEPDILICDEPTSALDISVQAQILNLLKELQQQRGISYLFITHNMGVVSYIADDVL
                     VMKDGLAVEYGACEKVLRQPEHPYTRQLLNAVLDIE"
     gene            13055..14935
                     /gene="parE"
                     /locus_tag="ACF2SP_000481"
     CDS             13055..14935
                     /gene="parE"
                     /locus_tag="ACF2SP_000481"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFD2358288.1"
                     /translation="MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSV
                     DEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSD
                     KNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
                     TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQG
                     LTDYLQQTLPEDYLPEEPFAGAFKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGG
                     THVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKE
                     RLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQ
                     GPALPGKLADCLQTDLSQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVD
                     SSQVLASQEIHDISVAIGVDPGSSDLEGLRYGKICILADADSDGAHIATLICALFLRH
                     FKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIIQHLSQTTKSKVNVQRFKGL
                     GEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRAADRKLWLETKGNLA
                     EI"
     gene            complement(15269..17386)
                     /locus_tag="ACF2SP_000482"
     CDS             complement(15269..17386)
                     /locus_tag="ACF2SP_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFD2358289.1"
                     /translation="MFRFLFPVSLFISSILLFSIQPMVAKAILPIYGGTPGVWTVCVL
                     FFQFILLIAYGYVWLLSQIKKPAVWRLIHMVLVVLSFTALPLLFHPAARDGQPEWVIL
                     HNLLIQLGLPLLVIGASAPLLQFAYSQTKSKGASDPYYLYISSNLGSLSALLFYPWVI
                     ERFIGLTRQFYLWNFGFAIYLLLLVTVLFFTKYQPLILTEKKEVDFLPWREIAYWIFL
                     SFIPCSLMLGVTLYITTDVAATPLFWVLPLALYLLSFVFTFTATPLISQKWISRNCLF
                     FLVFTILGFIFGVSQIRVWQLVLFNLLSFFILALLCHGQLFARRPKPHKLTLFYFCLS
                     IGGVLAGIFNGILAPNWFNYVYEYPLAILLSLFALQLPKTNRGWWVPLVVLGLLSLHY
                     FIPTVPWFKSTTTFHILAILALGIIVIWHRNKINLVLSMLILFVFLYFPPLQDKQILL
                     KERNFYGVKQVFKKDDVHALISQSTLHGMQVMNGEKASYGRSSYYGAIAPIVDILKKE
                     FHPLSATIMGLGAGTLLCQFRATDSIKVIEIDQQVIDLAKNNKLFTYIRDCSPSAEII
                     KQDGRLALVNMPDASQDLLILDAFNSDAIPVHLMTLEAFTLYKKKIVEDGVILVNLSN
                     RHLQMLPVINAIGRALNMITLFLAHKGDKKLGQFNSEWAMLTSNQSLAFQVMKGTNWK
                     FVADDDQFLWTDDYSNIIPLLKW"
     gene            complement(17643..18083)
                     /locus_tag="ACF2SP_000483"
     CDS             complement(17643..18083)
                     /locus_tag="ACF2SP_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFD2358290.1"
                     /translation="MSKVIKKLEVALADTYALYLKTQNYHWHVTGPQFKSLHELFEMQ
                     YKELAEAVDQIAERIRIMGHKAPATFAEFNQLKTIKDGDSGLSANEMVSELAKDNMMI
                     VKDLNQAIKIAQENEDEGTVTILSDRVAAHEKAHWMLSASKEKK"
     gene            complement(18206..19855)
                     /gene="groL"
                     /locus_tag="ACF2SP_000484"
     CDS             complement(18206..19855)
                     /gene="groL"
                     /locus_tag="ACF2SP_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFD2358291.1"
                     /translation="MMAKELRFGDDARLQMLAGVNALADAVQVTMGPRGRNVVLEKSY
                     GAPTVTKDGVSVAKEIEFEHRFMNMGAQMVKEVASKTSDTAGDGTTTATVLARSILVE
                     GHKAVAAGMNPMDLKRGIDKAVLAVTKKLQAMSKPCKDSKAIAQVGTISANSDEAIGA
                     IIAEAMEKVGKEGVITVEDGNGLENELSVVEGMQFDRGYISPYFINNQQNMSCELEHP
                     FILLVDKKVSSIREMLSVLEGVAKSGRPLLIIAEDVEGEALATLVVNNMRGIVKVCAV
                     KAPGFGDRRKAMLQDIAILTKGQVISEEIGKSLEGATLEDLGSAKRIVVTKENTTIID
                     GEGKATEINARIAQIRAQMEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRAQKALDSLKGDNDDQNMGINILRRAIESP
                     MRQIVTNAGYEASVVVNKVAEHKDNYGFNAATGEYGDMVEMGILDPTKVTRMALQNAA
                     SVASLMLTTECMVADLPKKEEGVGAGDMGGMGGMGGMGGMM"
     gene            complement(19880..20170)
                     /gene="groES"
                     /locus_tag="ACF2SP_000485"
     CDS             complement(19880..20170)
                     /gene="groES"
                     /locus_tag="ACF2SP_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFD2358292.1"
                     /translation="MKIRPLHDRVVVRRMEEERTTAGGIVIPDSATEKPMRGEIIAVG
                     AGKVLENGDVRALAVKVGDVVLFGKYSGTEVKVDGKELVVMREDDIMGVIEK"
     gene            20356..22116
                     /gene="dsbD"
                     /locus_tag="ACF2SP_000486"
     CDS             20356..22116
                     /gene="dsbD"
                     /locus_tag="ACF2SP_000486"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFD2358293.1"
                     /translation="MKKWLLFSLLFLTSLCVYAEPLPASEVFKVSVKKIDPNTFAIQW
                     NILPKYFLYSDRIQLNSDNDDIAQLGTLRFPTPLTKTDKQGRTFKVYRNQLNLPVGVL
                     GITPGETIVNLRFQGCADDGFCYPPEVKQIKLAIDDKLALSQVDLETFHAPEATPLEK
                     PEQDIADIFANHNWIMILLIFYGFGLLLSFTPCILPMVPVLSGIIVGHGKTATTKKAF
                     FLSLSYVLSMSVTYAIVGAVVALLGANLQISMQSPWAISLFSLIFVLLALSMFGFYEF
                     KLPDAWQSKIVGSSREQRGGHYLGAAIMGCLSTLILSPCVTAPLIGVLTYIAQTGNVL
                     LGSVTLFTLSLGMGTPLLLIGTSAGKWLPETGSWMNAVKAFFGILLLAVAIYLMARIL
                     PAGLVMGLWACLLIFSGIYSGALTKSNTNQEKLCQGIGIILLTYGLLILIGASMGSSN
                     PLQPLANLQAAPTVSNAFESAKAQSVKSVELAIKQAFGKPVMLDFYADWCASCKVMEN
                     TTFKDPRVQKALSHFIVIKVDVTANNKNDRALMQHFRVVAPPTFIFFNAEGVQLNNLK
                     RVGELNANEFMQTIKSIDEN"
     gene            22206..23525
                     /gene="rimO"
                     /locus_tag="ACF2SP_000487"
     CDS             22206..23525
                     /gene="rimO"
                     /locus_tag="ACF2SP_000487"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFD2358294.1"
                     /translation="MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVV
                     INTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYE
                     EVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
                     QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQIEWQGKTVNTRFYDLCE
                     QLGELGIWVRLHYVYPYPHVDDIVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSE
                     NTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVE
                     GAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIAR
                     SKSDAPEIDGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA"
     gene            complement(23595..24731)
                     /locus_tag="ACF2SP_000488"
     CDS             complement(23595..24731)
                     /locus_tag="ACF2SP_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2358295.1"
                     /translation="MKSHFSMQRLWAIIRKEFLLMKRDPATIVIMAILPLILVCIAGY
                     AINTYPQKVPTVLINLDNTDVTRELVREIQNTGYFSFVASTNDSESAHHLLKTGKAIL
                     VLTIPVNFTKALRRNQNPALLLEDGSIDAIASGRAIIALAGLKSSFIEKMGQKGINIP
                     LQRPDFQIINHRLYDPDRVTQFYVVPGMIGLVLMLTMLLITVVVAFRDVQGGTIEYLL
                     ASPTRPSEILLGEIFSYILIGYLQLSFGLILSYYLFHVPFIGAPLLLYLCTLPYIVAE
                     LSLGLTIATFCTSQFEAVQVINVFIAFSILLTGFTFPIFGMPEWAQYLGLFLPLTHFF
                     KIMFGIMLKGNNFGEIWANLWPLLAYCLTMISLATWRFKRHFVR"
     gene            complement(24728..25654)
                     /locus_tag="ACF2SP_000489"
     CDS             complement(24728..25654)
                     /locus_tag="ACF2SP_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358296.1"
                     /translation="MSKWSSKQIIIDVSGLAKSFNGINVVVNLNLQIEEGTIFGFLGP
                     NGSGKTTSLRLLSGLIIPDKGQGRCLNLDLMTQTKLIQAQIGYMPQNFCLYQNLTVYE
                     NLDFIARIYQLKNRKERLKEIIELLALSDNQQRITATLSGGWKQRVALAAALLHKPRI
                     LLLDEPTSGIDPQSRLLIWEHIQSIVSKGVTVLLSTQHMDEAERCHQLVYMSAGEIIA
                     RGSVIEIIQSTGLHSWVVKGESFSRLKDILVNNPAIQMIEKGNEMRISSIVDDIHQVL
                     DPFVLNNYEIKKTDTTLEDAFIYKIMKEGNPK"
     gene            complement(25647..26342)
                     /locus_tag="ACF2SP_000490"
     CDS             complement(25647..26342)
                     /locus_tag="ACF2SP_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFD2358297.1"
                     /translation="MVSFSKLFLIGWMRDLPNSGYPNRYCFADRITNTDDKRIRGMRI
                     GIAILVVLVCCTLQGCKKEKSPYQTTSYVESQLFIVSSPDGGYIKEWYADEGQLLHKE
                     DKIVTLDGVNSIKAPADSVMTERYYLKDEYVPPNFPIASLSLPSQMKILFYVPESHLE
                     KIKLGKKIRILLNEKKYSGKISFISNQAEYTPDAIFSEKNRYKLVYKVKADLSQGLRD
                     LLKIGQPVEVNYE"
     gene            complement(26287..27498)
                     /locus_tag="ACF2SP_000491"
     CDS             complement(26287..27498)
                     /locus_tag="ACF2SP_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD2358298.1"
                     /translation="MYANYYDMYQKTGIFSDEFFGQFLRTLGKCYVQCADVGECYATL
                     SKITDKDGESWYKAWYEMANTLKTKANISWEANHFITAAYAYLRAAEYYRASEFFLRA
                     NPDDPRVIACFDQLQFCFERAMHRLHPGAQKIWIPYDDQTLSGYLFFSDQSPKATLIV
                     PGGYDSTFEENYALVPAALLHGYHILIFDGPGQGHVLRRQKLYMRADFEHVVSQVVDW
                     LDEQSELGTSSYVLIGRSFGGYLAPRAACSEKRLAALICDPGQMDIAGSLNKMLPPNL
                     MMLLKEGKADELNACFYQLFSENPMKEFYFKSRMYTHGLATPFDYLQALSAYTFVDRV
                     QEITCPTLVCDNPSDRISNRGNTLYEALRCEKNYVMFDAKLGAGMHCEADANGQFQQV
                     IFDWLDERFTQ"
     gene            complement(27763..30504)
                     /gene="glnE"
                     /locus_tag="ACF2SP_000492"
     CDS             complement(27763..30504)
                     /gene="glnE"
                     /locus_tag="ACF2SP_000492"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFD2358299.1"
                     /translation="MSPQSDIETLISTKSWLIEKYVAPLHHPLRESAEKLILVSDYAC
                     RQIRLLVSLLESDSCCSLWSRESYFYAIQQISLELSQAQYMQELRRFRHTHFLRLLLL
                     EIADLASTQEVMQSWSDLADSIILHTLKYIGFTLLSRYGVPRNSEGKEIQLYALAMGK
                     LGGRELNYSSDIDLIFAFNEVGSTDGEEQITNQHYFSKMVQTFVHLLQSVTPDGFVFR
                     VDLRLRPNGDSGPLVSSFAAMETYYQEQGRDWERYAMVKARVIAESLHEPFPWFERLI
                     IPFVYRRYVDFGVIESLRGMKAMIEREVQLNPLLNDIKRGRGGIREIEFIIQNIQLIR
                     GGRLPQLQVQNAMLALAVIKKEKLLPRCDALKQAYLFLRKLENILQSLNDQQTHSLPE
                     DQLKKVQIVYAMGFTDWEQFFNKLQQYQRIVSNFFHSILGKVDDYEDEKRLLNNQLFS
                     LWQGHVESNMAVNLLISLGYENAQHCYQMLHAFRHGPRCRRLSQGARIRLDRFMVLLL
                     TELTYCPKTDEVLLQVIHLLENIVGRSAYLALLTENPHALRELLFWFVNSPFISSLFV
                     SQPFLLEILLEQEKDWHPDSLSQMEQTIVEKLEHTNDVEVHEEILRQFKLTNWMVAAR
                     AELYGLCDAVRIGKFLSDVAQVIVSQVLIIASKQLCGRHPEMAQIQSRFAIIAYGKLG
                     SREMSYASDLDLVFLHSAKPSEEALVTRLTQKILHMLTTRTQMGVLYHVDTRLRPSGA
                     AGLLVSHVDAFVEYQKTQAWTWEHQALLKARILSGNSKIKNQFSQLKKSVLLLERDSK
                     LLQRDVLTMRSKIDQFQESDEVKLARGGLLDLEFLVQFLILNLRDPKFSSYTNTLSQL
                     KHLFLSNVLTKSQFTTLKKAYQYFHYLMHQKIVQSDVINDDEMQNAVSSVCKDLYRY"
     gene            30642..31394
                     /locus_tag="ACF2SP_000493"
     CDS             30642..31394
                     /locus_tag="ACF2SP_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2358300.1"
                     /translation="MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTN
                     NELFGYDIATMTELCKIMKRQCNFRVMRFRQLIPALINKQIDVAISAMSITSERSNLV
                     NFSMPYLLSYSRFLTNEVHNTHQPFNLMLLKGKRIGVEQNTVFSQEVRRIGIIDPVIV
                     EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRE
                     NSVLLKSLNDALVEYQRSDKYKENYNKYMMNF"
     gene            complement(31649..31915)
                     /locus_tag="ACF2SP_000494"
     CDS             complement(31649..31915)
                     /locus_tag="ACF2SP_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358301.1"
                     /translation="MSQNWPTRDKDLQTARVIMEEYANERDSDSLGLFEIVVDQNEKR
                     MNFRLSGWVVILAKHFTSLYGVSQGDYVTRQVISRCITQGQTLH"
     gene            32213..33040
                     /locus_tag="ACF2SP_000495"
     CDS             32213..33040
                     /locus_tag="ACF2SP_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFD2358302.1"
                     /translation="MHKIINGAMTGIGLRAPHYAQILEEKPDIDWLEVHTENFLVKGG
                     PLLELLDSISQHYPLSFHGVGLSLGSAEGVCIAHLNRIKTLIDRFQPALLSDHLSWST
                     IGNTYLPDLLPIPYNEESLTIISNNIDRVQNYLKRTLLIENPSSYLEYQTSSYSEPDF
                     LAEIIKRTEARLLLDINNIYVSCSNNKQDPYQYIQRIPHEAVQEIHLAGHSIVYQDNT
                     NFLRIDTHNNYVCHEVWDLYRYAIHYTGLVPTLLEWDTEIPSLDTLLNEARKSACYA"
     gene            33033..33848
                     /locus_tag="ACF2SP_000496"
     CDS             33033..33848
                     /locus_tag="ACF2SP_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFD2358303.1"
                     /translation="MPRMKQIQEFFLNTVLNQQDTAIFKYFCPNEITNEFRFDIYRNT
                     ILQNLRHSLEMTFPAIWKLVGKGCADGLALHFIQDKNNLPTTNCLDDWGEKFPLFLKN
                     NPTVSHLLYLKNIAEIEWLKHLSYCSRDCLVFDPQKLQKHLNDRVEKLILLFNPSVFL
                     YSSSYYLKNIFDLIENPTETGSIDFQQIPSYAVISRQKNQTVIHWITHDLFHFLCHIK
                     AGASLGFAYENTLENNPDFDLLTALQFLLRNELLEDSFIHSNYHLTHANNCPE"
     gene            complement(33868..35181)
                     /locus_tag="ACF2SP_000497"
     CDS             complement(33868..35181)
                     /locus_tag="ACF2SP_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HFD2358304.1"
                     /translation="MDSYSKLPLRIRKEIEAQQYTGELLVAGVQLGVIVLLSIINIFS
                     SAGYSPGAPVQSSSLGLWLFAILILLRLWFVYTNQLNPIFLGFFIVAEMALLLFIIWT
                     YYLQFETTPTINLKNPHINYVYVLIALRALRFEPVWVILSGLTAALGWGIIAWQTLSS
                     SGMNVITWDYVTFASTRSVYLGAVFDVILSVLLVTTIIALVLTRAKHTLFQAVEQTSA
                     AKDLSRFFDTSVVEKITTSEKSLQAGYGELRQAAIMFIDMRGFTKASVKLSPSELIGL
                     LAEYQRLLVPIIQKNNGSIDKFMGDGILASFGAVTPSNTYAADALRTVDEIVASVDVW
                     SENRRKKEQIVVGVGIGLASGEVVVGVIGYENRLEYTVIGEAANLAAKLEKHNKEEKT
                     QALTTRDTFLEAVKQGYEDKITKKELNARNVGGVGEPLDLVVLAK"
     gene            complement(35174..35446)
                     /locus_tag="ACF2SP_000498"
     CDS             complement(35174..35446)
                     /locus_tag="ACF2SP_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HFD2358305.1"
                     /translation="MNKRPIQNTAVIAAALGAALASIYTYTDWLSSDEITVKRERCYN
                     VARAGKNDCATSQHSCAAQSTADRDPEAFIMLPKGLCERIVGGRSG"
     gene            complement(35483..36247)
                     /locus_tag="ACF2SP_000499"
     CDS             complement(35483..36247)
                     /locus_tag="ACF2SP_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HFD2358306.1"
                     /translation="MDKYLSANSLEGVIDNEFSMPAPRWLNTYPAGPYRFINREFFII
                     AYETDPDLLQAILPPDMELLEPVVKFEFIRMPDSTGFGDYTESGQVVPVRYKGEEGGF
                     TISMFLDCHAPIAGGREIWGFPKKLAKPKLFVEEDTLIGILKYGSIDIAIATMGYKHR
                     PLDAEKVLESVKKPVFLLKNIPNVDGTPLVNQLTKTYLTDVTVKGAWTGPGSLELHPH
                     ALAPISNLYIKKIVSVSHFITDLTLPYGEVVADYLA"
     gene            complement(36289..38505)
                     /locus_tag="ACF2SP_000500"
     CDS             complement(36289..38505)
                     /locus_tag="ACF2SP_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2358307.1"
                     /translation="MQQEDQAQIPQNSRSFSRNTARCFDTFMDTVDFLGRIGWPVIDL
                     AFRIWIAQQLLVSAILLSNHWDTAVYLAKNEYPVPWLSPEWEALLGILAQFVGGISLL
                     LGLFTRCGAAVIIGFAVMTQVYYQPIDLNLLWIALMLGYVLRGPGPLSLDNLFEHGFS
                     RSPLPFAMLIVTFVDRTREFFSSLYLFSLRMWLMISLLLINQHIQLISSDALPLLMPW
                     LPLNSASIVFNNVSLFMAIFLGLGLATRVVAIFGLIVVLLSFTPGVTGFYLYWMVLMA
                     ILFISGPGILSLDHLIFHALKKHYPQLSGKPAFSLDGLPRVVIIGAGFGGIACAKALR
                     HMPVKITLIDRHNYHLFQPFLYQIATGSLSPADIAISIRSIFLEQFNAEILLGNVTGI
                     NKEERLVIADNFTIPYDYLVIATGATHSYFGKDSWAPYAPGLKTINDGTSVRSRIIKS
                     FELAEIAESDEERKQFLNFVIVGAGPTGVELAGAIAELARFGIVKEFRHFDPASANII
                     LVQAAPRILPTFSEQISQKAQRYLESMGVKVLVNSMVEQIDSDGVIINKERIYSKSVF
                     WAAGVAASPASKWLQLEADPAGRVKVNDDLTVAGYSNIFAIGDTAASNAWNGKPVPGI
                     APAAKQGGAYVAKVISKRIYNNNSRYKPFKYIHYGSLATVGRKAAVAEFDRFKISGEL
                     AWWFWGGVHVFFLVGSRNRLSVILNWFWSYYTFRANNLLITDDSLNKKNKSGTSEV"
     gene            complement(38621..>39151)
                     /locus_tag="ACF2SP_000501"
     CDS             complement(38621..>39151)
                     /locus_tag="ACF2SP_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2358308.1"
                     /translation="NKKISADAVMASACLPFMFQAVNIGEDYFWDGGYMGNPAIFPLI
                     YNSECRDILILHINPIYREHVPDTAAEILNRVNEISFNSSLMREMRAIAFVTKLIDSG
                     WIKDEYKNNLRKLYLHAIRADVSMESASVASKLNPEWSFISHLYEEGRKKGEEWLKSN
                     FKHLGKVTTIDLDEYL"
BASE COUNT        12324 a         8194 c         6885 g        11748 t
ORIGIN      
        1 agagataaaa ttatcaccgg gaaaaaaatt atcttttcac aatccgttgc aaaagaccaa
       61 acaaaggatt tatcaaactt tttagcagaa cggttttact ctgtaaatca aagtcataat
      121 catgccatta ttatcggttc cagtctttct caccaagaga atgatatcga acatgataca
      181 atactcgata catccggcgt gttagtgact actgatacaa atggcattgt taacggggca
      241 agagttgcaa ctactgacgg tctgggtggt ggaaatggcg atgaggaaga agatgacgag
      301 atatacaaag tatctcattt cagttgcgag aattttttga attgcgacca aaatatagat
      361 acgactctaa acttgattac ccaaccaaag gcctctgaaa aaaaacaaac agcaccgaaa
      421 acattgcaac atacagaagc ttcgatggcc gcttttattt atcaaaatca tccgggcaaa
      481 ggatacatag gtgaatttgc aaatattggt gatggactaa ttattatcct ggataaacgt
      541 tttaagataa agcatatggt atccgcatgc catatatatc gtggatttgg tacctggact
      601 ccaccatcac tgcaagccct ggcaacaact gcaaataaag acgccctgct tgtcagacaa
      661 acacttaaac ttgcagaagg cgacattata atatcaatga cagatgggat ttggggggag
      721 ctgaaaacca gtttgattgc tcaaaccaat gaccgtcgag atataggagt ggataaagaa
      781 tatttcaaaa cgctatttga cgaattgaca gactccccct acccttcttc atttgatatt
      841 gctcgcatca ttaccagtag cgcaatgtct cgaagtttgg aacgcagaaa aacattaata
      901 aaattaatta atgaaataga acaacaacac ttccatgaaa aatctgtcaa aaccattaat
      961 gaagtcctgg aatattttct taagacaggc catgtggaaa cagcacagac attaaaagca
     1021 attctctttg aagacggttt gagtgatgga ataacttatt ttgaaaatat tgaaatcccg
     1081 ctggagatgg taatgcacga tttaaaaagt cgtaccgtcg gtgattgttc aaccatcaat
     1141 gtaacgcgca ttccctatca tttagatgag ctcatacgaa gttttatcaa ttaccctgag
     1201 aaacatcaaa tcctggcgcc actatttaaa gcaaggataa aatctgaggc tgatttagaa
     1261 gaagcatttc atcgcctgtc acttgaaacg gtacaagctg aaatcgagtg cccaatttct
     1321 gaagctcatt ttgaacgtgc ttttaaaaaa gaaacccttg ataaaactca ggctgtctta
     1381 actcactatt ttcgtatttc gactggttta gatagtaaaa aaaattatca ggaacgatta
     1441 aatgatctga gtgcatactt ctccaaggaa tcctctctgg aaaaaaatga tattaaattg
     1501 ctattatcca tgctagatag tgaaataaaa cctaaaaccg gggttttcca aacattgttt
     1561 ggggaaaacc aaaataaatt atacaaggca tttcacaaaa aaatcgaatt gcaattattg
     1621 gatggtgaaa tagaaaataa aaatgaattg aaatagcagt tcttaccatt ttaatttcaa
     1681 ggatgtacca actgcttcct gaatagactt tttatcgact ttcctcgaaa tgtcctggtt
     1741 tttaattaat aattttagtt cgccgtcaaa aaatttctgg cttcttttga tttcttctaa
     1801 tccttcgcca ggaagattac cgagcatctc tgtgagttta ctatcgtaga gttcctttaa
     1861 aacagataaa acctcatcat gtgctttggc atcgtttgat tttcctttaa aaaaggatac
     1921 tccttctttc ggggcataac ctgtaggagt cctaatcctg ttatttttga tttggacata
     1981 caaatcatta atttgtgcca tagcaattag aggattttca tcgagctctt tctttaaatc
     2041 gttcttaaat ttaaaaggac gttccaaaat agaattggca tcggagtttt taagaaattt
     2101 tgatgaagaa gatatattgg tatgggcacc tgctttcatc agtttgacac ataaatgaaa
     2161 atactgcttg gataatgcaa tatctaatat tgtttttcct ttaatgtctt ttttatttaa
     2221 atcgactcct tcttttaaaa ggaaatcact taaattcatg aagtgtattt cttcagtttc
     2281 ttttgaggat ggaaatgtag gcatctcatg caatgcatga tgaaaaggat ttcttctttc
     2341 agagtttctt ttatgaagta aagtactgtc tttcttgaca atatccaata taattggcaa
     2401 gtcacatcgc ctcatgacac tatgcaaaat attattaccc agcctgtctt cgaaatcgac
     2461 ccttgccccc ttttccaata aaattgttgc cattttcaaa tcattaacta ccacagcatt
     2521 ataaagaggt ggatttccat tagcatttgg ctgattaatt aatttcttat cgttctcagt
     2581 tataatattt actaaagctt ccctgttttc tgcctttgaa taaaaaacat aatgcaggat
     2641 agaattacca ctggtatcgg tcgtactttt attagctcct tttttcaata aataactcac
     2701 taatttgaga tgaccggaat ttacagataa catcagcggt gttgcttgct ctttggttaa
     2761 gggtatatca atatctaccc cggcacgtaa gaatttttta accagactga cacttcctaa
     2821 agaaatagct atcctgaagg cttctttatt caaaagaatt ttctcatttt tggcaattat
     2881 tttacctaat gtttctttat ccttccgagc ccatatcgcc tccaaaagct catcactgat
     2941 atcagcacca gaatttaata gttcatcgaa aattttcaat ttatgatttt tatatgccaa
     3001 agtgattgct gaagagccac tgttatccgg ttcattaatc tgaataaccg ctgtggttgt
     3061 caaggcagat attatttccg gggttccaaa ctcagcagca taatggaatg ctgttttgcc
     3121 atgattatcc ttgatattca gcactttgat ggcgtcttcc tgacttagca caacttttag
     3181 tatttttccc ataatttgca tatttccaca ctctgcagca tagtgaagag ctgtttttcc
     3241 atcataatcc tgaagagata aatccgcttt ttttgcgata agctcatcaa tcattgccat
     3301 atttcctgat atgacagcca aatgagcagg agttttacca acataaagag ggggtgctcc
     3361 ttgctctata cgttgattaa tctgagattc atcgcgattt gacaataata attttatctc
     3421 accgggatca ctaagataga ttgcgcctct gtgtaatgga tattgctcat ttaatgatga
     3481 taaacatttt tgagtcttcc gatgcagttt ttcaggattt aaatctgaca agtccatatg
     3541 tttgattttc tcatacgatg ggctatccag catgtcccga aatttggttt gctcttccaa
     3601 gcttgaatga tatccatata aagtaatggg agtctttttt ttactttgtt caataatttc
     3661 tactgtatta aaaatcgctt ccttcacaac aacaactaat tcctcagcgc tatataaatc
     3721 aaagacatta ggttcgtaaa acgtcgctgg cggtaaacct tgttgcatta aaagaatatt
     3781 taataaatta ttgacaaagg tacgaccatt ggcatctcga aaaggatgta atacttcgta
     3841 cattctaatg tgcttaacga taattttaag tttttcatca agagtttcgg cgatttttat
     3901 ttcctgattg aaagattggg taattttttc caggtaaaca tccacatttt tcattactgc
     3961 aagataaaag tgattagtat tactatgccc atatttgcac atatcgaaat agatttgctt
     4021 ggcaagattg ggtatttgtt ctggactcag atttttaaaa tagttcttat caaagcatgg
     4081 gccaaatgga cccgctttgc ctgggccaaa tgcagcctcg ccttctgtaa gaaataccaa
     4141 gctcaggaat tcttcgatac ccttaattga ggcccggcca gctggaatac caaatgaaac
     4201 aggttcattt gttctgagtt cacctgggtt tttttcctgt aattcctcca ccttttttcc
     4261 acacttcttg tgtattttct ttattaaatc aacgcttaac tcaaattcct ctcctgattt
     4321 ctcaacttgc aatgcaatag aacataaact ctccagagcg gcgttaaggc atcctggctc
     4381 tctcgactca tagcctctcc acccattttc ttttgaccag aaacgtccat caacaaataa
     4441 tctccatatt tcttcccgtg gataggcttg aaaaaaatat aatccaggta ggtttggcat
     4501 agttcttacc aattaaaaat atcaacttat taataattat aggacaatgt ggtcaaatat
     4561 ggtcttttga gaagagctga gaaactaagg acctaaacta aacatatcgc aaattgaagg
     4621 gtgataagta tataatatcg accttgatta tttatagaaa tccaggaata gacatgcgcg
     4681 catctcaatg gtttttggtc actcaaaaag aaactcccaa cgatgcagaa atagcatccc
     4741 atcaattaat gctaagatca ggaatgattc gtaagcttgg ctcaggactt tatacctgga
     4801 tgccacttgg ccttagagtg cttcgtaaag ttgaaaatat agttcgggaa gaaatgaaca
     4861 aaactcatgc catggaactg cttatgccat cagtacaacc tgctgaatta tggcaagaga
     4921 cgggacgttg ggaaaccttt ggtggccaat tattaactat gaaagacagt aatcaaagag
     4981 agtactgctt tgggcctacc catgaagaag tcattaccga tatcatgcgt aatgaattgc
     5041 aatcgtataa acaattgcca gtcaattttt atcaaataca aaccaagttt cgcgatgaaa
     5101 tcagaccacg gtttggggta atgcgagccc gtgagtttat tatgaaagat gcttactcct
     5161 tccacctcag tcttgaaagc ctgcaagaaa cttataagga tatgtatcaa gcctattgca
     5221 ggatttttga tagaatggga ctcaaatatc gagcagttga agctgatacg ggagccattg
     5281 gcggttcagc gtcccatgag tttcaggttt tggctgaatc gggtgaagat ctgatttttt
     5341 atagtgacgc cagcgactat gctgcaaata ttgaacaagc gaccagctta aaaccaccca
     5401 aagcgaatca agcatgcaat gaaacaatca cattggtcga tactccaaat caaaaaacaa
     5461 ttgatgaagt agccagcttc cttggtattg ccagtaatca aaccatcaaa accttgattg
     5521 tcaaaggcaa ggaacatcct atggtggctc tggtcttaag aggagacgat gagctcaatg
     5581 aagtcaaagc aaccaaacat cccttagttc actctcccct gtcctttatt gatgaagaac
     5641 tgattttaaa aacgttaaaa acccctttag gctccatagg gccaatccag ttaaacatcc
     5701 ctgtcattgt tgatcatcat gccttagcca tgccttcctt tgtctgtggc gccaatcagg
     5761 cagataaaca ctttatcaat gctgcatggg agcgcgatgc aaaatacgat gatgcctatg
     5821 atttacgcaa cgttaaagaa ggtgatcaaa gccctgatgg aagaggaacg cttcactgct
     5881 gtcgtggaat agaggtagga cacgttttcc aattgggtga taaatacgcc aaagccatga
     5941 atgcgtctgt tatcaatgag caaggacaat tacaaaccat gatcatgggc tgctatggat
     6001 tagggatcac tcgtgtagtc gctgccgcga ttgagcagca tcatgacgaa cacggaatca
     6061 tctggcctca agctttagca cctttccaag tcaatattat tcctcttaat ggcgcgagat
     6121 cacaaaccgt taaagaacag gcagaatcac tttatcagca actcaaatct catggcatcg
     6181 atgtattact cgatgatcgt aatgaaagag caggtgtatt atttgctgat aatgacttga
     6241 tagggattcc tcaccgatta gtggtcagtg agagaaatct ggaacaaggc tgtattgagt
     6301 acaaatcccg aacttcaagc gaaactcaac tgatcaatct ggataaggtg gttaatttta
     6361 ttattgagtt aattaataaa taagattttc taccccattg acaccgcgtt atctgcattc
     6421 cggcaatgct gttcggatta aaaataaccc gaacggcatt aaagagaagc caataataca
     6481 ggacttgtat ttaaagaatc acaaataaag ttctttgccc aggttcacca tatctcgttt
     6541 ggtattcatt aatgtgagtt atggataaga tcagttgtca cctcgaatcg ttcgggctga
     6601 ggaggcattt atgccgtctc gaagccttgt acgggattta atgttgtgcg ccaaggcttc
     6661 gagacgatgc ttatgcatct cctcagcccg aacgaacctt ggatatcaac tggttttata
     6721 atccttatcc atcactgacg ttaaatacta aaaaccattg gaggttgcat tacagaatcc
     6781 gtctgaaact cctcttcagg attggaaaac gccccctcac cacaagaaag tatcctgttt
     6841 ttagattcta tctgaacctc atttactggg gctattggag gcgcggtttt aacacgtttt
     6901 agtttagcct caaaatcata aaaatcattg aggttatcaa aacgcttgat aatcacattg
     6961 gttttccttt cttcttcaag aatcttatcg cttaaaaatt gaattttttt gttaccagag
     7021 tccatctgtt gtgccagatt gctttggatt aaaaccattt cattaaccaa cgcatcatat
     7081 tttgtcatta tttttttagt taacaacgat tgcaaaatgc tatctgtgcg tccttgtaat
     7141 tcattaaatt gatacctcca atcatcctct gtctcttcct gtctgaacat gctgggaatc
     7201 aaaccaacaa tcctgcgagt cactgtagaa aaccatgatt gggactgttc ttcatcgacc
     7261 ttatgcattt tattatataa ttccgtaagg tccgctttta actgctcatc ctttatctta
     7321 tcaatttgct ccatggtcat cgtatttcta tccatgtttt ttaacgtatt tctgtcaaga
     7381 aaatgatttt gcatgttttc aggaaattga ctgaggtttt caggtatttg gccattaatc
     7441 cactccttta gtgttaaaat ctccttcacc ttgcttcggc attgctctgg aaacaactct
     7501 acttcgggat tgcccttgat gcgatctttc aaaaaaggta agtgtttggc atggattcta
     7561 ttcagattca gcaactctaa tctattttgc ttaagagaaa attctaactg acggtactcc
     7621 tcttttaatt cagttaacct ggatttatat tgcataaacc cttcaaataa tgcggatgtc
     7681 ccttctcctt caataattaa ctcaacatac tcattaaatc ctgtatatcc tttttttact
     7741 ttcacgttaa aaacagaata tccttctatt ccctccagct cctccttaat ttttcgcgct
     7801 ttaaaatcaa aatatttcga ttcatctatg atatcctggt gtttttcctg aaaactattc
     7861 caaagagtac gaatcatccc tcgtaattgc actagaggct cacgcaatag ggaatatgaa
     7921 atggataatt cattgatttt tttacaaaga acataataca tttcattcaa tgtataaacc
     7981 ttgcgactct cattttgctt ttgctgaagt tgccttttaa ccttttgcca ttcagaatga
     8041 gcagttaaga tctgactgtt atttttttca aagtcttcta aaacagagct gaatttctct
     8101 cccaacaaat agtattgagc ttcttctttg cactcctgaa taaaattaat atccagccca
     8161 ccctcatctc tacccagaac agttaaactg tccatggtat ttaatgtttg attaatcaaa
     8221 tcacgtgtat ttttatgatc aaatgaaaaa atccaaacat cctccaattg agcttgatgt
     8281 ttgcataagc ctctatataa ttttttgata gcaatctgtt tggataaaga atctcttgct
     8341 tgttttactc cgttggccaa aaccaatatt tcattcgccg ctttttttac tgctgaccgc
     8401 tcaatccaat accaaggtcc tctgttttca ggcttagccc aagccaatgt cttgtcaaga
     8461 tgatgaatga tttgtgtagc tgtactatcc agataggttt cttttaaaat cttaagttct
     8521 ttatgttctt caaaacctgc ttgcttatac actctcagca tatcactgat aattggttgc
     8581 atgcggaatt tttcgctcaa tttctctttg tctaaccatc tgctttcata atcaattaat
     8641 aacaactcca aagcaagaaa gttatccgca ttattagctc tctctattag gccttgttta
     8701 acacaggcag gtaatgagga gttattgatt tctttgctaa tatccctggc ccacaatttc
     8761 agttggctgt gcacaaaagc taatcgatag ggctctaaat caccatcatg gtagcttaac
     8821 atcgcaccag ttacttctac tgctgactca aggtgacgat gactgcgctt tttctcatga
     8881 gttgcttctt ttttctttaa acgagctgat aatttactcc actttatttg ttcacttaaa
     8941 tcgacctctt ccaaatattt acaacgcaat gcattgactg agtcactggc gaatctattt
     9001 tctgtttttg ctttcaactg atctcgattt aggttccaaa tgctggaaac agatttttca
     9061 taggcttgaa ttgccttttc caattctctg gtttgtaagc gctgaaaaac atccctacgg
     9121 acataggggt ttggatacat ttttttagga tcagaatctt ccaaacactt tgtccattgc
     9181 ttctttagcg aatcaatcaa ctcatttctt tgcgcaaata attcattatc cagctgtctc
     9241 cattctgact tggggtaaat taaatgcaaa aatgcttgcc attcttccaa ttgatttaat
     9301 accgcttgtt gaatattaga aacagattgt atgaaatact gtttaactgc ttcttctctt
     9361 cgtctttcag ccaaactttg cgtatttaat tcttcctgca aattcttcct ggcagggaaa
     9421 acccgatacc ccacatccat atcgaaccgg ctgatttgcc caaccacttc accaacggtt
     9481 tcctctattt cggttatcct gatgacacgc cccttccctt tataataatc atacagtgaa
     9541 gaaaaatgag ataaagttct atcgtctaca ttagaaaaat caatatgatt aagaataagg
     9601 tagcttgaaa caggatatcc gcatctatcc atagacagtt cctcatcctc tatcactgcg
     9661 tgcgagtgat aagaggtcaa atcagaaagt gttgagtaat taataccccc aacccggtaa
     9721 gtaccagact cagaatttgc ctctaccaca gaggaaggta tacccaaaca ggtaaagaaa
     9781 tctctatcac ccttctcaag ataatcctga gctattaatc cgtgatttgc aaaacacagg
     9841 tcaacggtac caccatcagc aagcacccat tgcatggcag caagaattgc tgcaggcgga
     9901 gcgctcaggt tttcattaca actgactccc attaacacat tttgttcttg atgatgtaaa
     9961 gacaataaaa gagaaatcat ttgtgtagta tcaaagaata tgccggtact gcgaaaatac
    10021 tgctctcgca tcactgcgag taacttcagt tgagttcgca gtttatcctc tttgctaata
    10081 tcgagtcttc tcatctccat aattaaaaaa tcagacaact cctgcaactt attcctgcct
    10141 aattgggtta aatcagtata cgtttcattc gctattttta gaggaaaatc cctgcctatt
    10201 gcattgataa atacaacttg ttgagccaat tcatattgcc ctttacacga taaacccgat
    10261 ccatcaacca aactttcaat cttggcaata acctcttgaa tttgacttgt atcaaattgc
    10321 tcattaagaa tttgttctct cgatttaaca agaatacctt cttgattcac tttgggagcc
    10381 ctggatccgg caggatctct gtcaaatgaa tcaatatata attttaactc tcttgagtat
    10441 tcacgcaatt tctcggaaaa aacatccaat gcgggataag gaggataatc aaataaagaa
    10501 gaaatatctt ttagtaaaac actatggatc tcaaggcgag actgaacatt gaataataaa
    10561 tcatcattta cactctcaac atttcttcca agtatagtca gtatttttat ccattgttct
    10621 aaggatttct catccgtttt ttcaagctta tcgataagac ggcaatatct ggtcaattct
    10681 gatttgtcat acttcttact tgatttaatg agtaccgtta ataaggaaat caactcagga
    10741 aggtctgtcg atttggagtc aataatcgct gatgtggatt gaataaattg tatgagcagt
    10801 tcatgctcga catccaagcc agccttttcc actgcctcaa tcacagcatc aaataaggat
    10861 tgtgatttgt tttcatcgat tcccttcaga atattgaact cgattaattc cttcaacagc
    10921 ttgatctctt tgttaatgaa ttcaatttgt tcatcatttc ttgttagaca accaccaaat
    10981 tcctttacac ctttgaaatt taattgattt cgagtgattt tcatgccctg aattccctga
    11041 aaaatagaat gccctcatta tatataagct gcataaaatg tagcagataa atgtaaaaga
    11101 aaatttgaaa tattactcga tgtccaatac agcatttaac agctgcctgg tataagggtg
    11161 ttctggttgc cgaagtacct tctcgcacgc tccatattca acagccaagc cgtctttcat
    11221 gactaataca tcatcagcga tataagagac gacccccatg ttatgggtta taaacaaata
    11281 ggaaatacct ctttgctgtt gcaattcctt taataaatta agaatttgtg cctgtacaga
    11341 gatatcaaga gcactggtcg gctcatcgca gattaaaata tcaggttctg tcgccaaagc
    11401 tctcgcaatg cagatccgtt gtctttgtcc accggaaaac tgatgagggt atcgatgcag
    11461 actggtaatt ggtaagttca cttgctcaat tagggatttt tgttgtttcc tgatgtaaga
    11521 aggcttcatt ccctgtgcat gcatcccttc tgcgatgatc tctccaactg tcatgcgtgg
    11581 attcatagaa gaaaaaggat cttgaaaaat aatctgtact ttttttctgt atcttcttaa
    11641 agcgcttcct gtcatagaca gtacatcact atccttatat ctgatttccc ccccggccgc
    11701 tggcaataac ctcaacagag cacggcttgc agttgttttc ccacagcccg actctccaac
    11761 caaagctaat gttttacctc tggagagagt gaacgacaaa ccatcaacgg ctttgaaaat
    11821 ggttttctta cgactcaaaa atcctttctt atggataaaa tgaactgata aatcatctac
    11881 agagaacaca atctcttctt tttcaacgga aggaacccaa ctgattttat ttttatccag
    11941 taatggcaat tctttgatat caggatacaa atgacatctc aagatacgct gctcaacttc
    12001 ttgtaattga ggctcctcat gatgacaacg ttcaaaagca taaatacagc gtgtatggaa
    12061 acgacaacct gaaggcatag actccaaaga aggtaccgtg cctgcaatga tagacagttt
    12121 ttcatttctt ttggcaaatg aaggtaagga tgtcaaaagt tgctggacat aaggatgact
    12181 cacttttgaa aaaaaatctt ttactgaggc ctgttccact acctgccctg catacatcac
    12241 acaaactcta tcagccatgg ttttagccac gcccagatca tgagtaatta ataacaaact
    12301 catctgatgt ttcttttgta ttttttttag caaggccaat atttgagcct ggatagtcac
    12361 atccaaagcc gtagtaggtt catcggcaat aaggatatct ggctcgcaag ccaacgccat
    12421 agcaataacc accctctgct tttgaccacc agacaattga tgcgggtatt gataaattct
    12481 catttgtggt tgaggcattt ctacctcatt caataacaat attactcttt cttttatttg
    12541 ctcttttgtc aaagaccgat gcctttttaa aacctcagct atctgctctc caatcgttaa
    12601 tacgggattt aaagccgtca tcggctcttg aaaaatcatc gcaagcctgc gccctcttaa
    12661 ctcccgcatc attttctcag gtaaatttaa gatgtcttca ccgtgaatat tgatttggct
    12721 gtcaatccca tagacaccag ctctgggaag taatctcatc atagccaatg aagttaatga
    12781 tttaccacat cctgattctc ccagtaaaac caaagtctcc cctggattta gactaaaact
    12841 taatttatcc aaagccagaa ctgttttatc agggctttta aaagcaactg acaattgatt
    12901 aattttagcg gtgttttgca tggagtttgc cacaaataat gataactaca ctctaacaag
    12961 agaggggtga tagtagcaat aaaaatacca taccactctg agcaaaagaa tgtttatgat
    13021 agaataagca caattaatat ttgagtgaat acccatgtca gaaaactaca ctgcggaagc
    13081 gattgaagtc ttaagtggtc ttgagcctgt tcaacgacgt cccggtatgt ataccgacac
    13141 gacacgcccc aaccatttgg ctcaggaagt gattgataat agcgtcgatg aagtacttgc
    13201 tggatttgcc agccgtatta ttgttacttt acatgaagac ggctctattg aagtcgagga
    13261 tgatggccga ggtatgccag tcgatttgca tcctcaactg ggcttaagtg gtgtcgaggt
    13321 cattatgact cgacttcatg ccggaggtaa gttttcagat aaaaattaca gcttttctgg
    13381 cggcttgcat ggcgttgggg tgtccgtggt aaatgccttg tcagaacgac ttgacgtcac
    13441 cataaagcgc aatgggatca tttatcaaat gacctttgct aatggcgaca aattatgtga
    13501 attaaatgaa accggggtta caaaaaaaag agagaccggg actgttatac gtttttggcc
    13561 taatggcaaa tattttgaca ctacaaaaat ttctgtaaaa catctgactc atgtattaag
    13621 agcaaaagcg gtattgtgta gtggcttgtc catgaccttt atcaataaaa tcactaatga
    13681 agaaatgaac tggtgttatg agcaaggttt aactgattat ttacagcaaa ccttgcccga
    13741 agactatttg cctgaagaac cttttgccgg agcattcaaa agtgatgaag caactgttga
    13801 ttgggctttg gcctggtcag actctgccaa tggcaattta aatgaaagtt atgtgaattt
    13861 aatcccgaca atacaaggtg gcacgcacgt caatggattg cgttcgggat tatttgaagc
    13921 catgtctgaa ttttgtgagc tgagaaacct tcttcctcgt ggagtaaaat tgacagcgga
    13981 tgatctatgg gaatcatgcc agtatgtttt atcaatcaaa atgaaagagc cgcaatttgc
    14041 ggggcaaacc aaagagcgct tgagttcacg ccaaacctca ggactagtca gtaatgtcat
    14101 aaaagacgct tttgcaattt ggttgaatca gcatcgcaac cagggtgaag ccatagcagc
    14161 tctcgcaata gaacgcgcac agaaacggct aaagcaagcc aaacaagtgg cacgcaaacg
    14221 tgttagccaa ggacctgctt tacctggaaa attagccgac tgcttgcaaa cggacttaag
    14281 tcaagcggaa ctattcctgg ttgaaggaga ttcggcagga gggtcggcca aacaagcccg
    14341 caataaagat tatcaagcca ttttaccttt acgtggaaaa atacttaatt catgggaagt
    14401 agattcttca caagttctgg catcccagga aattcatgat atatctgttg ctataggtgt
    14461 agaccctggc tccagcgatc tcgagggtct tcgctatggg aaaatctgta ttctcgctga
    14521 tgcagactct gatggagctc atattgccac actaatatgt gctttgtttt taagacactt
    14581 caaacctctg gttaaagcag gccatgtttt tgtagccatg cccccgctct atcgaatcga
    14641 tgcaggtaag ctgattcact atgccctgga tgatgaagaa aaaaatagaa tcattcaaca
    14701 tcttagtcaa accacaaaat caaaagtgaa tgtgcagcga ttcaaaggat taggtgaaat
    14761 gaatcccatt cagcttagag aaacaacaat ggatcccaat acaaggcgtc tggtacagct
    14821 tactcttgat gatgaagagc agacggaagc cattatggat atgatgctca acaaaaaaag
    14881 ggccgccgac agaaaactct ggctagaaac aaaaggaaac cttgctgaaa tttgagctaa
    14941 ggtgcattag caattggcca cccctttagc gatagccatt atcggctact tttctatctt
    15001 atccttggta atatggatag taaccgtaca cggggtatgc aaatttttct gcttataagc
    15061 ctatttattt gagttgtctt tcccgcgaag gcgggaagct aactaaaaat caacccttgt
    15121 gctagcttta tggatagatt cccgccttcg cgggaaagac agaggaccta atattcctta
    15181 attacatacc ccatgaacag ttactatgga tagcccgcat cacgataaag caaggataca
    15241 attgaatttg atagcttacc tttttaccct accatttcag caaggggata atattagaat
    15301 aatcatcagt ccataaaaac tgatcatcat ctgctacaaa tttccagttt gttcctttca
    15361 tcacttgaaa cgccaaggat tgatttgagg tcaacatagc ccactctgaa ttaaattgcc
    15421 ccaacttctt atctccctta tgcgctaaaa ataaagttat catattcaaa gctcgcccaa
    15481 ttgcattaat taccgggagc atttgcagat gtctgttact tagattaacc aaaataactc
    15541 catcttcgac aattttcttc ttatataagg tgaaagcttc aagtgtcatc aaatggacag
    15601 gaatagcatc tgaattaaac gcatccaaaa ttaacaaatc ctgtgaagca tctggcatat
    15661 taaccaaggc caatcgtcca tcctgtttaa taatctcagc tgaaggcgaa cagtctcgta
    15721 tgtatgtgaa taatttatta tttttagcta aatcaattac ttgttgatca atctcaataa
    15781 cctttatgga atcagttgcc cgaaattggc acagtagtgt tcctgcaccc aaccccataa
    15841 tcgtcgcaga aagcggatga aattccttct tcaaaatatc aactattgga gctatggccc
    15901 cataatagga gcttcttcca taacttgctt tttccccatt cattacctgc atcccatgaa
    15961 gggttgattg gcttattaat gcatgcacat catccttttt gaagacttgc ttgacgccgt
    16021 aaaaatttct ttccttgagt aaaatctgtt tgtcttgcaa cggaggaaag taaaggaaaa
    16081 caaataaaat gagcatggat agtacaagat tgattttatt acgatgccaa atcacaataa
    16141 ttccaagtgc caaaatagca aggatgtgaa aagtggtagt acttttaaac cagggaacgg
    16201 taggaatgaa ataatgaagc gacagcaaac ccaaaacaac taatggcacc caccatcctc
    16261 ggttagtttt cggcagctga agagcaaata aacttaataa aatggctaat gggtattcat
    16321 aaacataatt aaaccaattt ggcgctaaaa ttccattaaa aatccctgct aaaactcctc
    16381 cgatagacaa acaaaaataa aacaaagtga gtttatgtgg cttgggtctt cgcgcaaata
    16441 actggccatg acacagtaaa gccaaaataa aaaaacttaa cagattaaat aacaccaatt
    16501 gccacactct gatttggcta actccaaaaa tgaatccaag aattgtaaaa accaggaaaa
    16561 acaggcaatt acgagaaatc catttttgtg aaataagggg ggtcgctgtg aaggtaaata
    16621 caaatgacaa aaggtaaagc gctaaaggca aaacccaaaa taagggagtt gcagcaacat
    16681 cagttgtaat atataaagta actcccagca tcaaactgca agggataaaa ctcaaaaaaa
    16741 tccaataggc tatctctcgc caaggcaaaa aatccacctc ttttttttcg gttaatatta
    16801 aaggttggta ttttgtaaaa aacaagactg ttaccaacag taacaaataa atagcaaatc
    16861 caaagttcca taaataaaat tgacgagtca aaccaataaa tcgctcaata acccatggat
    16921 aaaaaagcaa ggcactcaag cttccaagat tactggatat gtacaaataa taagggtctg
    16981 aggccccttt gctttttgtc tggctatagg caaattgtaa taaaggagca gaagcaccaa
    17041 tcactaacaa aggcaaaccc agttgaatta acaaattatg cagaatcacc cattcaggtt
    17101 gtccatccct ggctgcagga tgaaacagca atggaagtgc tgtaaaactt aaaacaacca
    17161 aaaccatatg gatcaaacgc caaacagcag gtttcttaat ctgacttaaa agccatacgt
    17221 agccatatgc aatcagcaga ataaattgaa aaaaaagcac gcaaacagtc cacacaccag
    17281 gtgttccccc ataaattgga agtattgctt ttgcaaccat gggttgaata ctgaatagga
    17341 gaatggaact tataaaaaga ctgacaggaa ataaaaatcg aaacacaaag caatccttag
    17401 ccgatggtgc tgccaaatga atgaatcgat cattttacga ctacactctc gttcatacaa
    17461 ggatgaatta ttactctgca aaacaattta taaacataat tgatagggat gtttcacaaa
    17521 cacatattat tccatattga ctgatggaaa aatccattta tggtttttga agcttttgta
    17581 aacatcccat tacttgaaga taggcactga ttccatatca actgatacat aaaatgaata
    17641 ctttatttct tttccttgga ggctgataac atccaatgag ctttttcatg agccgcaact
    17701 cgatcgctta gaatagtcac agtaccttcg tcttcatttt cctgggcaat tttaatcgcc
    17761 tgatttaaat cttttactat catcatatta tcttttgcca actcactgac catctcatta
    17821 gcactcaatc cggaatcacc atctttaatc gttttcaatt gattaaactc agcaaaggta
    17881 gcaggagcct tatgccccat gattcgaatt cgctctgcaa tttgatccac tgcttccgca
    17941 agttctttat attgcatctc aaataattca tgcaaacttt taaattgagg tccagtaaca
    18001 tgccaatggt aattttgtgt tttcaggtac aacgcataag tatcagcaag agcgacttcc
    18061 agctttttaa ttactttact cattattctt tccttgttat ttattgacgt tattaaggat
    18121 agttcaaaac agtcaaagaa caaaatagac attaataaaa aacccgcctt tacagcgggt
    18181 tttttattgg ataaccggga gaaaattaca tcattccgcc catgccaccc attccgccca
    18241 tgccgcccat atcgccggca ccaacacctt cttctttctt aggcagatca gcaaccatac
    18301 attcagtagt caacatcaaa ctggctacag aagctgcatt ttgcagagcc atacgggtta
    18361 ctttggttgg atcaagaata cccatctcaa ccatatcacc gtattcacca gttgcagcgt
    18421 tgaaaccgta gttgtctttg tgctcagcta ccttgtttac tacaacagaa gcttcatatc
    18481 ctgcgttagt aacaatctga cgcattggag attcaatagc gcgacgtaaa atattgatac
    18541 ccatattttg atcgtcatta tcgcctttca atgaatcaag agctttctga gcacgaatca
    18601 aggcaacgcc accaccggca acgatacctt cttctactgc agcgcgagta gcatgaagag
    18661 catcttcaac acgagctttc ttctctttca tttcaacttc tgtggcagcg ccaactttga
    18721 taacagcaac accaccagct aacttagcaa cgcgctcttg taatttttct ctatcgtaat
    18781 cagaagtggt ttcttccatt tgtgcacgaa tttgagcaat acgagcatta atttcagttg
    18841 cctttccttc accatcaatg atagtagtgt tttctttggt aacaacgatt cgcttagcac
    18901 taccaagatc ttccagagta gcaccttcca ggctcttgcc aatttcttca gaaatcactt
    18961 gacccttagt caaaatagca atgtcttgca acatcgcttt gcgacgatca ccaaaaccag
    19021 gcgctttgac agcacatact tttacaatac cgcgcatgtt gttaactacc agagtagcta
    19081 aagcttcgcc ttcaacatct tctgcgatga tcaataaagg acggccagat tttgcaacac
    19141 cttccaatac ggacaacatt tcacgaatac tggaaacttt tttgtcaacc aataaaatga
    19201 atggatgctc aagttcacag ctcatgtttt gctggttgtt gatgaagtat ggagaaatgt
    19261 aaccgcgatc aaattgcata ccttcaacaa cagaaagctc attttccaat ccattaccat
    19321 cttcaacggt aataacaccc tctttaccaa ctttttccat tgcttcagca atgatagcac
    19381 caatcgcttc atcggaatta gcagaaatag ttccaacttg agcaatagct ttgctgtctt
    19441 tgcatggctt agacatagct tgtaattttt tggtaactgc taatactgct ttatcaatac
    19501 cgcgtttgag atccattgga ttcataccag cagcaactgc tttgtgacct tcaacaagaa
    19561 tagaacgagc caatactgtt gcagtagtag taccatcacc agcagtatca gaagttttag
    19621 aagccacttc tttaaccatt tgagcgccca tgttcatgaa acgatgctca aattcaattt
    19681 ctttggcaac agacacaccg tctttagtta cagtaggagc gccataagat ttttccaata
    19741 caacattacg gccacgtgga cccatagtaa cttgaaccgc atctgctaat gcattaacac
    19801 cagcaagcat ttgtaggcga gcgtcatcac caaaacgtaa ttctttagcc attatcattg
    19861 tctcctttaa acaattagat tacttctcga ttacacccat gatgtcgtct tcgcgcatca
    19921 caactaattc ttttccatca actttaactt cagtaccaga gtacttgcca aacaatacca
    19981 catcacctac tttaactgct aaagcacgaa catcaccgtt ttccaatact ttgccagcgc
    20041 cgacagcaat gatctcacca cgcataggtt tctcggtagc actatctgga ataacaatac
    20101 caccagcagt ggtacgctct tcttccatac gacgaacaac aacgcgatcg tgtaaaggac
    20161 gaattttcat acttttctct cctgattaaa ttaaacttta aatcatttaa gtattgcttt
    20221 ccttagggca aacaataccg atgacagcga tatggggaca ataaacaaac tttcaagggt
    20281 aaaatcaaaa aaaattttgg atttgggatt acagagtcta caataatgtt tattagtaac
    20341 tgtaaaagat atcgaatgaa aaagtggctc ttattttccc tattattttt aacttccctt
    20401 tgtgtttatg ctgagccctt accagcttct gaagtattta aggttagtgt aaaaaaaata
    20461 gatcccaata cctttgccat acaatggaat atcttgccca agtatttttt atatagcgac
    20521 cgtattcaat taaattccga taatgatgac attgcacagc ttggaactct tcgcttcccc
    20581 acccccttaa ccaaaacgga taaacaaggg cgaaccttta aagtttatcg caatcagctt
    20641 aaccttccag tgggagtcct gggcattacc ccaggagaaa caatagtcaa tttgcgtttt
    20701 caaggatgtg ccgacgatgg tttttgttat ccccccgaag taaaacaaat caaactggca
    20761 attgacgata aactggctct ttctcaagtt gatttggaaa cattccatgc acctgaagca
    20821 acacctcttg aaaaaccgga acaagacatt gctgatattt ttgcaaatca taattggata
    20881 atgattctgc ttatatttta cggatttggc ctgctcctgt ctttcacgcc ttgtatactg
    20941 cctatggtcc ctgtcctttc aggtattatt gttggccatg ggaaaacggc aacaacgaaa
    21001 aaagcgtttt tcctgtcatt aagctacgtt cttagcatgt ctgtcactta tgctattgtg
    21061 ggtgctgtcg ttgccttgct tggagccaat ttacaaatca gtatgcaatc accttgggct
    21121 atcagtctat tcagtctgat attcgttttg ctggccttgt ccatgtttgg cttttatgaa
    21181 ttcaaactgc cagatgcatg gcaaagcaaa attgtaggtt caagtcgtga acaacgtggt
    21241 ggacactatc ttggggcagc catcatggga tgtttgtcca cacttattct gtcaccatgt
    21301 gttacagcac cattaattgg agtattaacc tacatagcac aaaccggaaa cgtattactc
    21361 ggcagtgtca ctctatttac attaagcctc ggcatgggta ctccattact gctgatagga
    21421 acttctgctg gtaaatggct acccgaaaca ggaagctgga tgaatgctgt taaagctttc
    21481 tttggcatat tacttcttgc tgttgccatc tatctgatgg cacgcatttt accagcaggt
    21541 ttggtcatgg ggctatgggc ttgtctgctg atcttttccg gcatttactc tggtgcctta
    21601 accaaatcga ataccaacca ggaaaagctc tgccaaggca ttggtatcat tttattaacc
    21661 tatggattgc tgattctcat tggagctagc atgggctctt ccaacccttt gcagccttta
    21721 gccaacttac aagcagcccc tactgtaagc aatgcttttg aatcggctaa agcccaatct
    21781 gttaaaagtg ttgaactggc tataaaacaa gcgttcggaa aaccagtgat gcttgatttt
    21841 tatgctgact ggtgtgcatc gtgtaaagtg atggaaaaca ccacgttcaa agatcctcgt
    21901 gtacaaaaag ctttgagcca ttttatcgtt atcaaagtgg atgtgactgc caataataaa
    21961 aatgacagag cattaatgca gcatttccgt gtagtagccc ccccgacttt tatctttttt
    22021 aatgctgagg gcgtacaact caataatttg aaaagagttg gtgaattgaa tgccaatgaa
    22081 ttcatgcaaa caataaaaag cattgatgag aactaaagta actatgggcg cttttgtatg
    22141 aaatcagtcg acactggaac gattaagcag tataattagc tcattttttt tacagataga
    22201 agtttatgaa tcataaagta ggatttgtaa gtttaggatg ccccaaagca ctggtcgatt
    22261 cagagcgaat cattactcag cttaaagcac aaggctatga attggttccc acttatgagg
    22321 atgctggagt cgtcgtaatt aatacgtgcg gcttcattga cagcgctgtc caggaatcac
    22381 tggatacaat taaagaagcg atggctgaaa acgggcgagt aatcgttaca ggttgtttag
    22441 gagctaaagc ggacgtaatt aaaaatgcct gtcctgatgt cctgcatatc agtggtgctc
    22501 atgcttacga agaagtagtc aatgcggtac accaacactt accgcctcct gcagacccct
    22561 ttacccaact gattccccca cagggaataa aactcacacc gcggcattat gcttatttaa
    22621 aaatatcaga aggatgcaac caaaaatgca ctttttgcat tatccctacg atgagaggaa
    22681 aattgcaaag ctatccaatg gcccaaattc ttactgaagc taaaaaatta aaacaagccg
    22741 gagttaaaga attactggtt atctcacaag acaccagtgc ttatggcgtg gatacacgtt
    22801 atcagcagat cgaatggcaa ggaaaaacag tcaatacccg tttttacgat ctatgcgaac
    22861 aattaggcga attaggaatt tgggtgagac tccattatgt ctatccctat ccccatgttg
    22921 atgatattgt tcctctaatg cgcgatggac tgattttgcc ttacctagac ataccattac
    22981 aacatgccaa ttcacgcata ttaaaagcaa tgaaaagacc tgccagcagt gaaaatacct
    23041 tattgcggat tgcttcatgg agagaaattt gccctgacat tacgttacgc tccacattca
    23101 ttgtaggttt ccctggtgaa acagaagagg agttttccga attactagcc ttccttaaag
    23161 aggcacaact agacagagtc ggttgtttca aatactcccc tgttgaggga gccaaagcaa
    23221 atgatttgga taatcctgtt tcggaagata ttaaagaaga gcgttatcat cgttttatgc
    23281 aagtacaagc agaaattagc cgtaataaat taaaaaataa aattggcagt actcaaaccg
    23341 ttttaattga tgagataacc gaagatcaaa tcatagctcg cagtaaaagc gatgctcctg
    23401 aaatcgatgg tcttgtttat ttgcctaaaa tatccgggat tactgttggg tctttcgcag
    23461 aagcaatgat aaccgacagt gacgactatg acttgtatgg tgatttggaa tactctcttg
    23521 catgaaagaa atggtattaa aacagtactt tttctgctca tcttcattgg caagagagag
    23581 aatatttatt gtatctagcg tacaaaatgc cttttaaatc gccatgtcgc taaagaaatc
    23641 atggtaaggc aatacgcgag tagtggccat aaattagccc aaatttcacc aaaattattt
    23701 ccttttaaca taatgccaaa cataatctta aaaaaatgcg tcaatggcaa aaataatcct
    23761 aaatattgcg cccattcagg cattccaaat atcggaaaag taaatcctgt taacaaaata
    23821 gaaaaagcaa tgaaaacatt gatcacttgc actgcttcaa actgggatgt acaaaaagtg
    23881 gctatagtta aacccaacga taactccgcc acaatataag gtaaggtgca taaatataaa
    23941 agaagagggg cgccaataaa cggaacgtgg aataagtagt aagacaagat aagaccaaaa
    24001 cttaattgta aataaccaat gagaatataa gaaaaaattt cacctaataa aatttctgat
    24061 gggcgggtcg gtgaggctaa taaatattca atcgtaccgc cctgaacgtc tctaaacgct
    24121 acaaccacgg ttatcagcag catcgttaac atgagcacca agcctatcat cccgggaacc
    24181 acataaaatt gagttacgcg atccggatca tataatcggt ggtttatgat ttgaaaatca
    24241 ggcctctgca agggaatatt aatacccttt tggcccattt tctctataaa tgatgatttc
    24301 aatcccgcca aagcaatgat agctctacct gaagcaatag catcgatact gccatcttcc
    24361 aataagagag ctggattttg gtttcggcga agtgctttgg taaagttcac aggaatagtc
    24421 aataccagga tagccttgcc tgtcttcaat aaatgatggg ctgattctga atcgttcgtc
    24481 gaagccacaa atgaaaaata cccggtattt tgtatctctc gaaccagttc acgtgttaca
    24541 tcagtattat ccaaattgat taacaccgta ggaacttttt gaggataggt gtttatggca
    24601 tatccggcta tacaaactaa aatcaaaggt aagattgcca taatcacgat agtagcagga
    24661 tcacgtttca tcaataggaa ttctttgcgg atgatagccc acaaacgttg cattgaaaaa
    24721 tgactcttca ctttggattc ccttctttca taattttata aatgaaagcg tcttctaaag
    24781 tagtatctgt ttttttgatt tcataattgt tcaacacgaa aggatccaat acttgatgta
    24841 tatcatcaac tattgagctg atgcgcattt cattaccctt ttcaatcatt tgaatagcgg
    24901 gattatttac taatatgtcc tttaatctgg aaaaactctc acctttaaca acccaggagt
    24961 gcaagcctgt cgattgaata atttcaatca ctgagccacg tgctatgatt tctccggcgg
    25021 acatataaac caattgatgg catctttctg cctcatccat atgctgtgtg ctgagtaaaa
    25081 cagtaactcc tttggatact atactttgta tatgctccca aattaacaaa cgcgattgag
    25141 gatcaatgcc tgaagttggc tcgtctaaca aaagtatacg aggcttatgt aacaaagcag
    25201 ccgctaaagc cacccgctgc ttccaacctc ctgataaagt agcagtaatc cgttgttggt
    25261 tgtcacttaa ggccagtaat tcaatgattt ccttcagtct ctctttcctg ttttttaatt
    25321 gatatattct tgcgataaaa tccagatttt cataaacagt caggttttga tagaggcaaa
    25381 aattttgtgg catatagcca atttgagcct gtatcaactt ggtttgggtc attaaatcca
    25441 gattcagaca tctaccctgg cctttatcgg gaattattaa tccacttaat aatcgtaaac
    25501 tggtcgtttt accactaccg tttggcccta aaaaaccaaa aattgttcct tcctcgattt
    25561 gcaaattcaa gttcactact acattgattc cattaaatga ttttgctaat ccggacacat
    25621 cgataataat ttgctttgag ctccatttac tcataattga cttccacagg ctgacctatt
    25681 tttaataaat cccttaatcc ctgagataga tccgccttta ctttgtatac taatttataa
    25741 cgattttttt cactaaaaat agcatcagga gtatattcag cctgattgga tataaagctt
    25801 attttgcctg aatatttttt ttcattaagc aaaattcgaa tttttttacc taatttaatt
    25861 ttttccaaat ggctttcggg aacataaaac agaattttca tttgagaagg tagagagaga
    25921 ctcgcaatag ggaaattagg aggtacgtac tcatccttta aataataacg ttcagtcata
    25981 acagagtctg cgggcgcttt gattgaattt accccatcaa gggttacaat tttatcctct
    26041 ttatgcagta attgaccttc atcagcatac cattctttta tgtatccacc atccgggctt
    26101 gacacaataa ataactgaga ttcaacatag cttgtcgttt gatacggtga tttttctttt
    26161 ttacaacctt gcaatgtgca acaaactaat acaaccagga ttgcaatccc tattctcata
    26221 cctcttatcc ttttgtcatc agtattagtt atcctatcag caaagcaata acgattggga
    26281 tacccactat tgggtaaatc tctcatccag ccaatcaaaa ataacttgct gaaactgacc
    26341 attagcatct gcttcacaat gcatacctgc ccccagtttg gcatcaaaca tcacatagtt
    26401 tttttcacaa cgcaaagctt catatagcgt attcccacga ttggatatcc tgtcgcttgg
    26461 attatcgcag accagtgtgg gacaagtgat ctcttgcacc ctatcaacaa aggtgtatgc
    26521 agataatgcc tgcaaatagt caaacggagt agccaagccg tgtgtataca ttcttgattt
    26581 gaaataaaat tctttcattg gattttctga gaacaattga taaaaacagg cattaagttc
    26641 atcagctttt ccttctttta ataacatcat aaggtttgga ggtagcattt tattgagtga
    26701 acctgcaata tccatctgtc ctggatcaca aattaatgca gctaatcgtt tttcactgca
    26761 ggccgccctt ggtgcaagat aaccgccaaa acttctgcca atgagcacat aactggaagt
    26821 ccccagttca ctttgttcat ctaaccaatc cacaacctga gagacaacat gttcgaaatc
    26881 agctctcata tacagctttt gtcgacgcaa aacatgccct tgtcccgggc catcaaaaat
    26941 caaaatatga tacccatgta aaagcgcagc aggaacgaga gcataatttt cttcaaaagt
    27001 actgtcatat cctccaggta ctatcaaagt tgctttggga gattgatcgc tgaaaaataa
    27061 ataaccgctt aatgtttgat catcataagg gatccatatt ttttgagctc caggatgcaa
    27121 tcgatgcatt gctcgttcaa aacaaaattg caattgatca aaacaagcaa tcacacgcgg
    27181 atcatcagga ttggcacgta aaaaaaactc gcttgcccgg taatattctg cagcacgcag
    27241 ataagcgtat gctgctgtta taaaatgatt ggcttcccaa ctgatattgg ctttggtttt
    27301 aagtgtattt gccatctcat accatgcctt ataccaactt tccccgtctt tatcagtgat
    27361 tttggacaag gttgcataac attcaccaac atcagcacac tgcacataac atttaccaag
    27421 agttcttaaa aactgtccaa aaaattcatc actgaaaatc cctgtttttt gatacatatc
    27481 ataataatta gcatacataa cagtaaaaaa ttccctaaaa attacagagt aactgaaagt
    27541 aaaatggatt gccatgtgtc agagtatatt acgcgcaggc ctatgaatta tcctaaatga
    27601 tattaggaca gacttacgaa aaccccaagg tcaaggcaaa aaatgttttt aatgagggag
    27661 tttagataaa ctaaatgact gaattaaaaa cattttttaa cgacgagatt ggggggcaaa
    27721 tctgtaggat tcattttcag gggagtgctg tctaaacaca atctaatacc gataaagatc
    27781 tttgcaaact gaagacactg cgttttgcat ttcgtcatca ttaataacat ctgactgtac
    27841 aattttctga tgcatcaaat aatgaaaata ctgataagct ttctttaaag ttgtgaattg
    27901 agatttcgtt aatacattcg ataaaaacaa atgcttaagt tggcttagtg tattggtata
    27961 acttgaaaat ttgggatccc taagattcaa tataagaaat tgaaccaaaa attccagatc
    28021 caataatccc ccacgagcta gctttacttc atctgactct tgaaattgat cgattttaga
    28081 tcgcatggtt aatacgtccc tttgcaataa tttactatct ctctctagca acaggactga
    28141 cttttttagt tgggaaaact gatttttgat ttttgaattt cccgataata tcctcgcctt
    28201 taacaaagct tgatgttccc atgtccaagc ttgtgttttt tgatactcaa caaaagcatc
    28261 cacatgactg actaacaagc ctgcagcacc ggaaggtctt aaacgagtat caacgtgata
    28321 caaaaccccc atttgagtac gagtagtcag catatgcaat attttttgtg ttaatcgggt
    28381 cactaaagct tcttcagatg gtttggcaga atgtaaaaag accaaatcga gatcagacgc
    28441 atagctcatc tctctactgc ccagttttcc ataagcaata atggcaaatc gcgattgaat
    28501 ttgagccatt tcagggtgac ggccacatag ctgtttgctt gcaataatta aaacttgact
    28561 gacaatgact tgcgccacat cagatagaaa tttccctatg cgcacagcgt cacataatcc
    28621 atacaattcc gctctggcag ccaccatcca attcgttaat ttaaactgac gcaggatttc
    28681 ctcatgaact tccacatcat tagtatgctc taatttttca actattgtct gttccatttg
    28741 ggataatgaa tcaggatgcc aatccttttc ctgctccaac aagatttcca acaaaaaagg
    28801 ttggctaaca aacagggaag aaataaaagg actattcaca aaccaaaata ataattctct
    28861 tagcgcatgc gggttttcag tgagtaaagc caggtaagcg cttcgcccca caatattttc
    28921 aagcaaatga attacttgta ataaaacctc atccgtttta gggcagtaag tcaattctgt
    28981 aagcaataaa accataaaac gatcaaggcg aatgcgagcg ccctgtgata atcttctgca
    29041 ccgtggtcca tgtcgaaaag catggagcat ttgataacaa tgttgtgcat tttcataccc
    29101 taaactaatc aataaattca ctgccatatt actttcaaca tgaccttgcc aaaggctaaa
    29161 taattgattg tttaacaatc tcttttcgtc ttcataatca tcaaccttgc caagaatgga
    29221 atgaaagaaa ttactaacaa ttcgttgata ttgttgtaat ttattgaaaa actgttccca
    29281 atcagtgaat cccatggcat aaactatctg cacttttttt aactggtctt caggtagaga
    29341 atgggtttgt tgatcgttca aactctgtag aatattttcc aatttcctca aaaaaagata
    29401 cgcttgtttt aaagcatcac acctgggtag taatttttct ttttttatca cagccagcgc
    29461 taacatggca ttttgaactt gcaattgagg taatctgcct ccgcgaatta attgaatatt
    29521 ctgaataata aattctattt ccctaatacc accccggcct cgtttaatat cattcaataa
    29581 ggggttaagt tgcacctcac gctcgatcat tgccttcatc cctcgcaggg attctatcac
    29641 tccaaagtcc acatacctcc tgtaaacaaa cgggatgatt aaacgctcaa accatggaaa
    29701 tggttcatgc aaagactcag caataacccg cgctttaacc atggcataac gttcccaatc
    29761 acgcccctgt tcctggtaat aggtttccat agctgcaaaa ctggaaacca aaggaccact
    29821 atctccatta ggtcgtaatc ttaaatccac tctaaataca aaaccatcag gcgttacact
    29881 ctgtaataaa tgaacaaatg tttgcaccat cttgctaaaa taatgctgat tggtaatctg
    29941 ctcctcacca tcagtgctgc caacttcatt gaaggcgaaa atcaaatcaa tatcggacga
    30001 ataattaagt tctcttcccc caagtttccc catggcaaga gcatacaatt gaatctcttt
    30061 accctcactg tttctgggaa ccccgtaacg actcaacaag gtaaatccaa tatattttaa
    30121 agtatgcaag atgatagagt ctgccagatc agaccaggat tgcataacct cttgagtaga
    30181 agccaaatcg gctatttcaa gtaataacaa ccgcaaaaaa tgggtatgac gaaaccgacg
    30241 caattcctgc atatactggg cttgagataa ttccaggctg atttgctgaa tggcataaaa
    30301 atagctttcg cgagaccata aggaacagca ggaatcactt tctaaaagag agaccaataa
    30361 tctaatttgt ctgcaggcat agtcacttac caatatcaac ttttcggcac tttctctcaa
    30421 tggatggtgc aaaggagcaa cgtacttttc aatcaaccag gattttgtag aaatcaatgt
    30481 ttcaatatcg ctttgtggag acataaaata tactattctt aatataaaaa cctaaacata
    30541 tcataggatt aatcaattaa ttttacattt aatactttaa aactcctatt ggacgttata
    30601 ctcttataaa agactctaat aatattttag tgaagatacc aatgaaattt attcagggat
    30661 tattgctttc tttctttttt ctagtttcac ctctccacgc tcaaaacgag cctatacctt
    30721 taatagtagg tgtaggaggc ttttaccctc cctttatcat gcaaggaacc aataatgagc
    30781 tttttggcta tgatatcgcc accatgacag aactgtgtaa gataatgaaa cgacaatgta
    30841 attttcgagt catgagattc agacaattaa ttccggctct gattaacaaa caaattgatg
    30901 tggcgatcag cgccatgtct attacttcag aacgttcaaa cctggttaat ttcagcatgc
    30961 cctatttatt gagttactcc aggtttctta ctaatgaggt acacaatact catcaaccgt
    31021 ttaatttaat gctcttaaaa ggtaaacgaa ttggagtgga acaaaatact gtctttagtc
    31081 aggaagtcag aaggataggg ataatagacc ctgtcatagt ggagtttgac agaaccgaag
    31141 aaatgcttga ggcattgagc gcaaataaaa ttgactttgt tctgatggat aatcctgccg
    31201 ctttatactg gtccgctaat tcgtcgggga gacttctggt aataggcagt cccatgctgg
    31261 ttggttatgg tttgggaatt gcagtcaatc gggaaaactc cgtattactc aaatccctaa
    31321 atgatgcctt ggtagaatac cagcgttccg acaaatacaa ggaaaattac aataaataca
    31381 tgatgaattt ttaagagtga tgtggttggg gcaattgcta caaaaattgc tcttaatcat
    31441 tcatcaatag ctgaataatc gttttaaggc ttaataagtc aacatcctga tatttaacta
    31501 ttctctaagg gcaggatctg agtccaggtt ggatgaaagt aatcatgaaa aatcacagca
    31561 tagatagcta tgtgagcatt tcgaaggatt attttctttc aatatgaacc caatacggcc
    31621 attccaattg agggctcatc tctagctttt aatgcagagt ttgaccttga gtaatgcagc
    31681 gactaatcac ctgcctggtc acgtaatcac cctgactaac tccatataaa gaagtaaaat
    31741 gttttgccaa aataactacc caaccagata aacggaaatt catgcgtttt tcattttgat
    31801 caacgacaat ctcgaaaaga cctaatgaat cactatcacg ttcattagca tactcttcca
    31861 ttatgactcg cgccgtttgc aaatctttat ctctggtagg ccaattttga ctcataagac
    31921 actccctaaa aacatgagca agactgttgt tacttctaca acatggaggc aattttgaaa
    31981 attgcaagtt gtgaaataaa taaatattat ctcataaaat gattaaatac tcaatattgc
    32041 ggttaatttt tacactgaat taaaaacatt tatggtttaa aatacccatt gaaaatggct
    32101 ttctccaaga ccattgcgtt cgaaacttct caagagtaca ttaatatatc attaaatctt
    32161 tgacatagtt ttttgattga tcaacaaaaa ttcaaatcgg ataaaaaata tcatgcataa
    32221 aatcataaat ggtgctatga caggtattgg cctaagagcg ccacattatg cccaaatatt
    32281 agaggaaaag cctgatattg attggcttga agtacataca gaaaatttct tggtcaaggg
    32341 tgggccttta ctagagcttc tagattctat aagccaacat tatccactga gctttcatgg
    32401 agtcgggtta tctttagggt ctgctgaggg agtctgtata gcgcatttaa atcgcattaa
    32461 aactctcatt gatcgctttc aaccagcttt gctttctgat catttgtcct ggagcaccat
    32521 cggaaacacc tatttacccg atttattacc tattccctat aatgaggaaa gtttaaccat
    32581 tattagcaat aatattgata gagtgcaaaa ttatttaaag agaaccttat taatagaaaa
    32641 cccctcttct tatttggaat atcagacttc atcgtattca gagcccgatt ttttagcaga
    32701 aataatcaag cgtactgaag ccaggctctt gttggatata aataatatct atgtttcctg
    32761 ttctaataac aaacaagatc cttatcaata tatccaaaga atcccgcatg aagcagtaca
    32821 agaaattcat ttggcgggcc attccattgt ctatcaggac aatacgaatt tcttgcggat
    32881 agacacacat aataattatg tgtgtcacga ggtatgggat ttgtatcgat atgcaataca
    32941 ttatacaggc cttgttccca cgttgttaga atgggataca gaaattccct cgttagatac
    33001 tttattaaat gaagccagga agtccgcctg ttatgcctag aatgaaacaa atacaggaat
    33061 tttttctgaa cacagtatta aatcaacaag atactgccat ttttaagtat ttttgcccta
    33121 atgagattac taatgaattc cgtttcgaca tttatcgtaa taccattttg cagaatctgc
    33181 ggcattccct ggaaatgacc tttccagcca tttggaaatt ggttggcaaa ggatgtgcgg
    33241 atggtctggc attacacttt atccaggata aaaataattt gccaacaacc aattgtttgg
    33301 atgattgggg tgaaaaattt cctctgtttt taaaaaataa tccaaccgtt agccatcttc
    33361 tttatttgaa aaatatcgca gaaatagaat ggttaaagca tttaagctat tgctcgcggg
    33421 attgtcttgt ttttgatccc cagaaactgc aaaaacacct aaatgatcga gtagaaaaat
    33481 taattttgct atttaatcct tccgtgtttt tatactcatc ctcttattac ttaaaaaata
    33541 tttttgatct gatagaaaat cccacggaaa caggttctat tgattttcaa caaataccaa
    33601 gttatgcagt gatatctcgt caaaaaaatc aaacagtgat tcattggatt actcacgatt
    33661 tgtttcactt tttatgccac atcaaagcag gggcctcact tgggttcgca tacgaaaata
    33721 ctctggaaaa taatcccgat tttgatttgc tcactgcatt acaattttta ctaagaaatg
    33781 aattattgga agattctttt attcattcta attatcattt gactcacgct aacaactgtc
    33841 ctgaataacc gtgaagagca acatgggtta ttttgccaat accactaaat ccaaaggttc
    33901 tcccacacca cctacatttc ttgcattcaa ctccttttta gttattttgt cctcataacc
    33961 ttgttttacc gcctctaaaa aagtatcccg ggtagtcaaa gcctgggttt tttcttcctt
    34021 attatgtttt tcaagctttg cagctaaatt ggcagcttcg ccaattacag tatattccaa
    34081 acgattttca taaccaatga caccaactac tacttcacct gaagcaagac caattcctac
    34141 accgaccact atctgttctt ttttacggcg attttcactc catacgtcta ccgatgcgac
    34201 aatttcatcc acagtcctta atgcatctgc cgcataagta tttgaagggg tcacagcacc
    34261 gaaacttgcc aaaataccat cccccatgaa tttatcaatg ctgccattat tcttttgaat
    34321 aatcggaacc agtaggcgtt gatattctgc tagcagtccg attagttcac tcggggacag
    34381 tttcactgat gctttggtaa accctcgcat atcaatgaac ataattgcag cctgacgaag
    34441 ctcgccatat ccagcttgca atgacttttc agatgttgtt attttttcga ccacacttgt
    34501 atcaaaaaaa cgtgataaat cttttgccgc gctagtttgt tctactgctt gaaacagagt
    34561 atgtttcgca cgcgtcaata ccaaggctat gatggtagta accagtagaa cggacaatat
    34621 gacatcaaat acagctccca gatagacact ccgagttgac gcgaatgtca cataatccca
    34681 tgtaataaca ttcatacccg aactggataa agtttgccag gcaataattc cccagccaag
    34741 ggccgcagtg aggccagaga gaattaccca tactggttca aatcgaagcg cacgcaaagc
    34801 aattaaaaca taaacatagt taatatgcgg atttttaaga ttaatggtag gtgtggtttc
    34861 gaactgcaaa taataagtcc aaataataaa taataacaaa gccatttcgg cgactataaa
    34921 aaatcccaag aatattggat ttaattgatt agtatagaca aaccacagcc ttagtaatat
    34981 caaaatagca aatagccata agcctagaga tgaggattgc accggcgcgc caggcgaata
    35041 gccagctgaa gaaaatatat tgataatgct taataataca atgacaccca actggacgcc
    35101 ggcaaccaat agttcaccag tgtattgttg ggcttctatc tctttcctga tgcgaagcgg
    35161 taacttagag taactatcca ctccttcccc ccactatgcg ttcgcataac cctttaggta
    35221 acatgataaa tgcttcagga tcacgatcgg ccgttgattg cgcagcacaa gaatgctggg
    35281 aagtagcgca atcatttttg cccgctctcg ccacattata gcaacgctct ctttttactg
    35341 tgatttcatc tgatgaaagc caatctgtat aggtgtatat agaagcaaga gcagctccca
    35401 aagcagctgc aatgacggcc gtgttttgta tcggacgttt attcatatga ttttcctgcc
    35461 atttttagag tcgcttttta ttttaggcca aataatcggc aacaacctct ccatacggta
    35521 aggtcaaatc agtaataaaa tgtgaaacgg atacaatttt tttaatataa agattagaga
    35581 taggagccag tgcatgagga tgaagctcca agctacctgg cccggtccat gctcctttca
    35641 ctgtaacatc agtcaaatag gttttggtca actgattcac tagaggagtt ccatctacat
    35701 taggaatgtt tttaagtaaa aatacaggct ttttaacgga ttccaatacc ttttccgcgt
    35761 ccagaggacg atgtttatat cccatagttg cgatggcaat atcaatactc ccatacttaa
    35821 gaatgccaat gagcgtgtct tcttcaacaa acaatttggg tttggccagc ttctttggaa
    35881 aaccccatat ttctcggcca ccagcaatag gggcatggca atcaagaaac attgaaatgg
    35941 taaatccgcc ctcttctcct ttatatctca cagggaccac ttgccctgac tcggtgtaat
    36001 caccaaatcc tgttgaatcg ggcatacgta taaattcaaa tttgactacc ggctccaata
    36061 attccatatc aggaggtaaa atagcttgca aaagatcggg atcggtttca taagcaataa
    36121 taaaaaattc acgattaata aatcgatatg ggccagccgg gtaagtattt aaccaacgtg
    36181 gagctggcat gctaaattca ttatcgataa ccccttctag agaatttgct gaaagatact
    36241 tgtccattaa tcactccctt tgtatttcct tattatcttc aatgagccct aaacttcgct
    36301 cgtccctgat ttattttttt tattcaatga atcatcggta atcagcaaat tattggcgcg
    36361 aaaagtgtaa taagaccaaa accaatttaa aatgacactt aaacgatttc gagagccaac
    36421 caggaaaaag acatggactc ccccccaaaa ccaccatgcc agttccccac tgattttgaa
    36481 acgatcaaat tctgcaacag ccgctttacg acccactgta gccaagctac cataatgaat
    36541 atatttaaat ggtttgtatc ttgaattgtt attataaatt cttttggata tgacttttgc
    36601 tacataagca ccgccctgtt ttgcagcagg agcaatacca ggaaccggct tgccattcca
    36661 ggcattcgaa gcagctgtat ccccaatagc aaaaatatta gaatagccgg ctacggtgag
    36721 atcatcattc actttcacgc gacccgctgg atcagcttcg agctgaagcc attttgatgc
    36781 aggggaagca gcaacacctg ctgcccaaaa aactgatttt gaataaattc tttccttatt
    36841 aataatcaca ccatcgctat caatttgctc aaccatacta ttcactaaaa ctttaacccc
    36901 catgctttcc aaatagcgtt gcgctttttg cgatatttgt tctgaaaagg taggcaaaat
    36961 ccgcggtgct gcctgaacca gaataatatt tgctgatgcc gggtcaaaat gacgaaactc
    37021 ctttactatc ccaaatcgag ccagttctgc tatggctcct gcaagttcca ctccagtagg
    37081 cccggcacct acaatgacaa aatttaaaaa ttgcttgcgt tcttcatcac tttcagcgat
    37141 ctcggccagt tcaaacgact ttattatacg gctacgaaca gaagttccat cgttaattgt
    37201 ttttaacccg ggagcatagg gggcccaact gtcctttcca aaataactgt gcgttgctcc
    37261 tgtggcgatg accagataat catatggaat tgtgaaatta tcagctatga caagacgttc
    37321 ctctttatta atacctgtta cattacccaa taatatttcc gcattaaatt gttctaaaaa
    37381 tatagagcga atcgaaatag caatatcggc tggtgataaa cttccagtag caatctggta
    37441 taaaaaaggt tgaaaaagat gataattgtg tctatcaatc aaagtaatct tgactggcat
    37501 atgacgtagt gccttagcac aagcaatccc cccaaaacct gcgcctataa taactactcg
    37561 tggcaaacca tccaggctaa atgccggttt tccagaaagc tgaggataat gtttcttcaa
    37621 agcatgaaag atcaaatgat ccaatgacaa gatccctgga cccgaaataa ataaaatagc
    37681 catcaacacc atccagtata aataaaaacc ggttactccg ggcgtaaaac tcaaaagaac
    37741 aactattaat ccaaaaattg caacaactcg agtcgctaag ccaagtccca aaaaaatagc
    37801 catgaatagt gaaacattgt taaaaacgat agaggctgaa tttaatggca accatggcat
    37861 caatagcggc agcgcatcag aagaaatcag ctgtatgtgt tgattgatga gtaaaagtga
    37921 aatcattaac cacatacgta aagaaaataa atacaaactt gaaaaaaatt ctcttgtcct
    37981 atctacaaat gtcacaatca acatggcaaa cggcaaaggg ctacggctaa acccatgctc
    38041 aaacaaatta tcaagagaaa gtggacccgg gcctcgcaac acataaccca acattaatgc
    38101 aatccaaagc aaattaagat cgataggctg gtaatagact tgagtcatga cagcgaatcc
    38161 aataataact gccgctccgc atcgggtaaa caaacccaaa agcagggaaa tcccccctac
    38221 aaactgcgcc aatatgccta acaaagcttc ccattcaggt gatagccatg gaaccgggta
    38281 ttcattttta gctaaataaa cagcagtatc ccagtggttg cttagaagta ttgcagaaac
    38341 taataattgc tgggctatcc atattcgaaa tgccaaatca ataacaggcc atccaatccg
    38401 gccaagaaag tcaacagtgt ccataaaagt gtcaaaacac ctcgccgtgt ttcttgaaaa
    38461 cgaacgactg ttttgaggta tctgtgcctg atcttcttgc tgcatatcaa aatccctttc
    38521 aagaaagttg ttaaaatatc tttgtatttc taataacttt ggatttaatt atagctgcaa
    38581 atttcaaatt atgttgtgga ctatcattct gataataaaa ttataaatat tcatccagat
    38641 caatagtggt cacttttcca agatgtttaa aattactctt caaccattct tctccctttt
    38701 tgcgtccctc ttcataaaga tgactaataa aagaccactc ggggttaagc ttactggcta
    38761 ctgaggcgga ttccattgat acatctgcgc gaatggcatg taaataaagt ttcctgaggt
    38821 tgtttttgta ttcatcctta atccaaccac tgtctatcaa tttggtaaca aaagcgatcg
    38881 cccgcatttc ccgcattaat gaagaattaa aactgatctc attcactcga tttagaattt
    38941 cggctgcagt atccgggaca tgctctcgat aaataggatt gatatgaaga attaaaatat
    39001 ccctgcactc ggaattataa atcagaggga aaatagctgg attccccata taaccaccat
    39061 cccaaaaata atcctcgcca atattcactg cctgaaacat gaaaggcagg caagcagagg
    39121 ccataaccgc atcagcagaa attttcttat t
//
LOCUS       DAXLDT010000012        37652 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXLDT010000012 DAXLDT010000000
VERSION     DAXLDT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 37652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37652
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.012"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..634
                     /locus_tag="ACF2SP_000502"
     CDS             <1..634
                     /locus_tag="ACF2SP_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358309.1"
                     /translation="DITKYSKWKHLIGKEQEYIWRSGLKHDCSKVMELEPIDGSYKNG
                     FGEIVDLEDNYIYPFLKSSDIGNSRIDFNRKTVIVTQKFVGEDTSMVKNIAPKIWQYL
                     ESYSNLLNGRKSSIYKGKPAYSIFGVGDYTFKSWKIAISGLYKKLNFCLIPPMKGKPV
                     IFDDTVNFLSFDSENEARFIYKLITSKIAEEFYSSMIFWDEKRPITTDIL"
     gene            714..1043
                     /locus_tag="ACF2SP_000503"
     CDS             714..1043
                     /locus_tag="ACF2SP_000503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358310.1"
                     /translation="MKVSSNAYKQGFWKEHSESWKASGLTQQAYCEQEGVSYRSFVYQ
                     HNRLASQSKKASLKFIEAKPGSVAINNQAAGLQLMLPNGIRIGIGAEINPELLQTVLS
                     VAGAVQC"
     gene            1037..1390
                     /gene="tnpB"
                     /locus_tag="ACF2SP_000504"
     CDS             1037..1390
                     /gene="tnpB"
                     /locus_tag="ACF2SP_000504"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFD2358311.1"
                     /translation="MLKLNEDTRIFVADKPVDCRKAINGLVSLVIEQFEQPVNDGSVY
                     VFYNGSCDRVKCLFWDKNGFVLYHKRLERGRFKMKKTADGAYVISQNQLDWLLAGLDF
                     QLMNEFKTLDFTQYF"
     gene            1473..3062
                     /locus_tag="ACF2SP_000505"
     CDS             1473..3062
                     /locus_tag="ACF2SP_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFD2358312.1"
                     /translation="MISISAQTLEQLQQENARLLALIGRRDNTIQNLQHQLHLFRTAR
                     FGRKSEKGVVPEQMALQFDEVYVASEVNASEEGSETQTETITYTRSKKSTGRKPLPKS
                     LPYVEQIHDLSDDEKQCACGCALTHINDEVTEQLDVVPQMTFRVVHIRKKYACKGCSD
                     TIRLAKLPKQPIDKAIATPGLLAAVINSKFNHHMPLYRQEAMFTSAGISVTRATLSNW
                     LIKSADLMTPLVKLMEADINDYDIAYADETSLQVLKEKDRAPTQKSFMWLFLGGPPDK
                     RAVVYQYHPTRGHQIPHDFFADFKGYVHADCYQAYVTLGQQEHIHHVACWAHARRYFV
                     DVAKAHKKQGVAHQIVALIGKLYQIERELKDKAATPALVFMARVKKSRPIVLQIKALL
                     DDAQLKVPPKSPLGAALFYSLTHWEALNIFLFDGRLELDNNLGERTIKPFVIGRKNWL
                     FHGNDLGAKAGSILFSLIETCKQNKIETFSWFKYVLTHIRQASTLEQLENLLPYNIDH
                     SLLTDMRSLPNLIMPEKSEVN"
     gene            complement(3318..3578)
                     /locus_tag="ACF2SP_000506"
     CDS             complement(3318..3578)
                     /locus_tag="ACF2SP_000506"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2358313.1"
                     /translation="MNMKKIIGSRITQARRANELTIKVLAERTGLGAARIGNWEQGTR
                     SPGSEEAMILSKELRVAASWLLCLTDNPSGEWIHYPKITFRA"
     gene            3745..4647
                     /locus_tag="ACF2SP_000507"
     CDS             3745..4647
                     /locus_tag="ACF2SP_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358314.1"
                     /translation="MGFLFVNTDELSALMGLPYIQQSAYLLGIRPYMDRNTFLVGVKR
                     KISYQQLAEALYVEPHQGFEYSGSPSRPQLRRIIYALERAGLVEIKSIGKQLILKCLL
                     ANSDLSAQNKPVTNPTHQPDTNPNDKNNIKSSSYDDKNQKPSTAQNTKPDTPHNSKDR
                     FIFLKQQFEKFWSLYPLKQDRTKAWREFGYLQPDEQMVEQILTGLQAQINNREEMEFI
                     GEWLPNWKYPATWLSRKCWNDELLTLKTQEEQQNEANQVRSRKKSATDILTESCKDAN
                     FDFDFDEPSDSGNNILSFDHTRVR"
     gene            4652..5011
                     /locus_tag="ACF2SP_000508"
     CDS             4652..5011
                     /locus_tag="ACF2SP_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358315.1"
                     /translation="MDTLFMRLAAIYGYLWVSTYSNPSFLELSKREWSESLNEFDNQV
                     LKLALEQIKKKLAVPPCLPIFYEYCTNIQKGIQARQEAIKQKTEPHIPTDPQVVQLHI
                     NQMKDYLFKEAKEKTSC"
     gene            5005..5223
                     /locus_tag="ACF2SP_000509"
     CDS             5005..5223
                     /locus_tag="ACF2SP_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HFD2358316.1"
                     /translation="MLTLIRRIGEAIYIDKGRIKVHLISENEGLIRLGIEAPKHVDIE
                     RKEVFVRKAVAQHEQAQALRNQSGGDDA"
     gene            5216..5629
                     /locus_tag="ACF2SP_000510"
     CDS             5216..5629
                     /locus_tag="ACF2SP_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358317.1"
                     /translation="MLKKTMVFSLVLCGLQTHAANITQVSRYATVKNQPLAAQINPMK
                     TVQQIHFPASIQTIGEAVDYWLGYSGYHLAPKEKQSESLQQVFQQPLPQVSRNLGPLT
                     IERGLTVLVGQQLFTLKQDDLLREINFSLIARSAR"
     gene            5626..6210
                     /locus_tag="ACF2SP_000511"
     CDS             5626..6210
                     /locus_tag="ACF2SP_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358318.1"
                     /translation="MKKYLDVKWLSLLVLNVLLITFVCVKNQSPANHNELNILGEQLT
                     SIQTQLKQPVEQPDLSLITQNLNQLNNFVQQLKNKDDHQLGEIFTTEQGAIKKQLEGI
                     TDLLRHLDEKKKPVKMLSASQLPFKVLSIDSIQEIPVASLAYHYKTQALEKGDTLAGW
                     TVFEIDFAKQIIEFENADKAHVLVRLNQHEVSHA"
     gene            6203..7024
                     /locus_tag="ACF2SP_000512"
     CDS             6203..7024
                     /locus_tag="ACF2SP_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFD2358319.1"
                     /translation="MLKLSTLAMGLVLGQQAIAGLYIPGIATSPMQQADNALAKAGLT
                     EFHDEVIEEKDFQLNDAQKQEAKVWGLNEKEEKRYLQLMQSRSGVYYKDLRMTPIDIL
                     GLNAKDDVEREHFASLAVRQEAQKVAQNIAWNNAFYKAYNELFKDVPVVGDFDPSPYS
                     PYAHQPIQLKTGETLYLFVRPDDAVTTIVLQLVDAINRTPNTKLNLLFLDMDNNAIQL
                     WANRHQIPIQLVTSQQVTLTSGNQQFESLTLAKKQTPLLLIANGKTSQVVDLGRF"
     gene            7024..7455
                     /locus_tag="ACF2SP_000513"
     CDS             7024..7455
                     /locus_tag="ACF2SP_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFD2358320.1"
                     /translation="MIRILFLLLVTLNVHSMNVIPLTLSQSDTSLKLNLSSPIGVPAK
                     SKATAGKVSRKTLDTSLLNMPLFVIGADKASIQWLEQHQEELKSMQATGFITNVNDFE
                     SIVALQEKFKLPLLPVNVDPLLAYLHEQHYPLIIAEGAVWQ"
     gene            7446..8078
                     /locus_tag="ACF2SP_000514"
     CDS             7446..8078
                     /locus_tag="ACF2SP_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFD2358321.1"
                     /translation="MAVKQYSRQLTMQLTIVLFLGWLAIIIWALSQWLLHGYSFAFEK
                     VNTLVNTQREAITPVYQGSLLAVLPLNSKPDWAFAIPYLNKLAINDLAKDLIEKSQQF
                     LQILLLSSQCLLIKLIILFAALPLFALTMIAGLVDGLNQRAIRTACLGRESSYVFHRL
                     NHYLKKGLVILLMLWLALPVSITPAYVFVPVSLLMGLMVAMTASRFKKYL"
     gene            8088..8414
                     /locus_tag="ACF2SP_000515"
     CDS             8088..8414
                     /locus_tag="ACF2SP_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFD2358322.1"
                     /translation="MKRILCLTLMIISLQCSANNPDLNETLVRIINQINAIMPLLDEA
                     QTQITPNARIQLQIEGFEDLQGQHHAGLREDLLNIRNGLIDYINQPIIAPRKIKPLEM
                     DFVRKP"
     gene            8414..8680
                     /locus_tag="ACF2SP_000516"
     CDS             8414..8680
                     /locus_tag="ACF2SP_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358323.1"
                     /translation="MSKDEIARMFASTFKQASNNISAQVFSSSIRFIAISLVILAVMW
                     CVNHMMGSEEKQQQGYLLNFGSRMVRLVIGLMIFILVLFVKETT"
     gene            8677..9042
                     /locus_tag="ACF2SP_000517"
     CDS             8677..9042
                     /locus_tag="ACF2SP_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358324.1"
                     /translation="MKTTLRTLYLGAVNLFYAPVLLADNWFPKISDADNIGDGSKSMM
                     SVTGNYVKQGLGLLLFITAVVTFIKFITTVQHGIEESKKNEGSMVAFGTFAVMGITYL
                     AISIASGYVGYTMITKFKI"
     gene            9054..9416
                     /locus_tag="ACF2SP_000518"
     CDS             9054..9416
                     /locus_tag="ACF2SP_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFD2358325.1"
                     /translation="MSQPSSRHLSHDFPAWKGLSLRELFWIVITTTPVTTLLFILLGT
                     MAGYPLAAGCIGFVIGFILSITVWPKGVARIKAGKPYGYVMKKTIQWMVRLRLKHSPW
                     IHYQGQWQKNKSVGEPHV"
     gene            9409..10065
                     /locus_tag="ACF2SP_000519"
     CDS             9409..10065
                     /locus_tag="ACF2SP_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HFD2358326.1"
                     /translation="MFEYWKKIDSLQHHNRLLLAFIGLLSAIVLALIINLMTMPKRYE
                     FWLSPNMAANGGLMKASDIPNEYVQGFVSSLIPTLYTWSNKGKEEFNQNIKAFHYYFT
                     SRHEQLLRETLGAYKNAQLFDRSQVASLYRFMEPQDIRKIGPDTWEVKLILRITQRLK
                     DNSGMVIADKVVAYHLRVVKLNLSRLHNPFQLALDGYTRPEERLQDLLVDTEESHETH
                     "
     gene            10052..10828
                     /locus_tag="ACF2SP_000520"
     CDS             10052..10828
                     /locus_tag="ACF2SP_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFD2358327.1"
                     /translation="MKLIKHMLLIIGFMLTQVSYALQSEHLVWEKVPLTIELPINKER
                     LVQFPQAIKIIDQQLSTNLDILKVKGSLYLKAKDTFNDARLIVQLLPEGEVIILNLKA
                     NEKAVNTTPIEILIDDPKVSHQSNASQYEYNAIQLTRFAIQALYSPERVRDIPEGIYR
                     SPMQTNKTIPLFYGASIEAHPLASWRGGSLYVTAVDLKNLLNQPVKLQFSKLMGHWQT
                     ASFYPKSELPPRNQHESTTVFVVSDQPFAAALTQHARYSR"
     gene            10825..12084
                     /locus_tag="ACF2SP_000521"
     CDS             10825..12084
                     /locus_tag="ACF2SP_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFD2358328.1"
                     /translation="MNKNKGIKILAGSVVFAVVMILINSRHSAPIHDDTPNPNNFHEA
                     ITSQFNDNIRDVSARLLETEKKLEQMHKENKSLQKQLKQPIQEASHTLQDELQTLKEK
                     LSALTDHQTQSYPMEGNQAPVSGQIKDLDGLLDKAPIESKIFADQDSSTKEEAPTKTP
                     FYTIPAGSDFSKVTLLSALMGEVPVEGKLMQPLFPFTAMVSRGDLMTANGMQLPEEII
                     GMKISGYSIGVGSFLDNISCVRAYVTSALFVFEDGHFVSAGQEQMKSSAELVNNDSIG
                     YLTTQFGNPCIKGQYITNAPRVLAAFMAAGGIQGAGNALSKWQMSYQAEGGSAIATPT
                     GDLAHFAAGGAINEGSQKITDWLEKRIQGSFDMVFVPASIRSHSGFIPNQFSLHFSQT
                     IAIDKEHQGRVLDYGYHQQKNFDNALR"
     gene            12041..12388
                     /locus_tag="ACF2SP_000522"
     CDS             12041..12388
                     /locus_tag="ACF2SP_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFD2358329.1"
                     /translation="MVTINKRTLTMLCASLALSACSTANISQSAIPEQGLTVSQLYQQ
                     SMQQPIQKAVVQQVRFEDSGEEFKQLPNPEVPIHIFAHVAQLGDEQIIKPAYTTRFFL
                     YKQNQYALASELY"
     gene            12398..15148
                     /locus_tag="ACF2SP_000523"
     CDS             12398..15148
                     /locus_tag="ACF2SP_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFD2358330.1"
                     /translation="MKRLSNWLLGEMNPQAIKEQTIKKQYANPLPSFADRLAIVDFNE
                     QEQVFLFADGKSLGSGFELGDIQAEAASPEYLQAVFNKIRDTFATVVPLHAIDPWVMQ
                     MFVQDEYCLDPVINHIKSKIPQQLVENPLTQDYLQRLQDLYNKMTRPEGLFLDPKTGA
                     PYRGRRRRIRVLFYRQLHQTTLPREQILLEHQEVVSQIETKLRSPGLEIKRLTGQDYY
                     QWWIRWFNPKSADEILEQYPYPNSIPAGFNLAQNIFFSPPESDEQGFIFEGRKQRILY
                     VDSLKEAPIIGLVSRERQQANPKHRYALLDTLPEGSIYTIQVVFSHDENLDSHLTRLE
                     KGIVGTSLKPQEVRDDIKTARNELSTGNRLFWVNQAVFYQAEDEQQAQVIEKNLHSIF
                     NDAKMPLIHSIYDIHPLNSWLNALPFNFDPHYARKYLCFDRLMYATELAALLPVYGRN
                     QGARHLPCFPFFNRLGEPVFFDLLHHDFISQNSHCAIFANSGGGKSVLTGWMIQSLLA
                     MKNARVVLFEMGNSFDRMLIHAKAHGKKTKQLLLSNKKEEAVPLNPFCEAYKAIPEIT
                     DNLSAEQAALIAQKIMDLKDPAHSEDLACGDGSRAYLAELALALRTMITEANALEEEQ
                     FTLADETLLIEVLIDAILTSFNNGIPQMLTEHIVSAFQRRLDKETVARKKDRILDMHD
                     RLNSYVINSAKSRFFNVPTEPLGDFDIFHIDISAIKDDTGKLALVMVSLLPRILAMAE
                     ATQNDNRPTFLFIDESHLQFQIPSVVTVCLLVAKVARKLGLWLVAITQNVTDMNSEKA
                     AKILSLIETWILLGLDEKEITDVKRFKSLTPQQETLIRDIDSQKGLYAEAVLLGSRYQ
                     GLFRVIPPRYLLALLMTEKSEKAQRHLLEKEHDVLVAAEIMAKKLEQPKTTTNNRAYF
                     YD"
     gene            15141..16115
                     /locus_tag="ACF2SP_000524"
     CDS             15141..16115
                     /locus_tag="ACF2SP_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFD2358331.1"
                     /translation="MTNTAFALESTSPPHPVNTFTIATRVLQKLFHNSHYKVIGSCTW
                     TVGRLPPRLEVTPAIEQFLPDLIITVSNKPEENPWLEARTLYENKAARTMYQQAYKAA
                     TGSALGFGNDSGQTTDLHINDERTRIVDVIGSPAAFYRIPYLSHRPETGFGVPYYLAE
                     ADAVMDRTEAAELLYMGTHPHLLINHEIGTLTQHWGSEIPRLMRVTQPYNYRASVVAA
                     MHAVDIVTNKNPLHVTKSTSNSCGKNCVVANAIYDPQNTKVIWQEIYPLNRNIHPGNA
                     QDFGIEDEKAGNGNYVFVLWRKYRGCIQHKGKLVNFLTFPKVGQPQKR"
     gene            16125..17492
                     /locus_tag="ACF2SP_000525"
     CDS             16125..17492
                     /locus_tag="ACF2SP_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFD2358332.1"
                     /translation="MKKSLLLALIVPTLTHAGSFMPSQSDYYYELGGTSNLFIPPVNK
                     DQTLVIGADIDGRMGFSCSGFNPVVSITNTFQDLKKSALNIPGGVIDNLKGSVAGFPL
                     YKLQQSMPALYNVLQNAASSAQNEFTLKVKDCQEVKQALEQNQSPMEGILSVSDSQGW
                     LDAAKRAKKENVDVTETSKSIAQKRDEYGLPWIGHDKGNAGGKFQRPIKVINDVVIAG
                     YNILLERKPLDSLEKPSTKIPMVQSWPTPTDAANWAVKVLGDIQVSSSENKQSHDAKA
                     GIGLSALLQSCANANTCSQNIAKALWNLVNGTWKLTEENLHKVSASNLLITDEVITTI
                     QHLPREEQMLTVSKLAEEIAVQNMLDKALMMRRILQAGLQVQEVQNLKPAMNMVLFAL
                     KKLDDDIHSLSFENDVRKKMMTETLGTIMDIRHQAQSQNMPGQDHEQPAVKNGAIYVK
                     ENKGA"
     gene            17495..19048
                     /locus_tag="ACF2SP_000526"
     CDS             17495..19048
                     /locus_tag="ACF2SP_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFD2358333.1"
                     /translation="MIVFNPLSLYTTYLGWQQYEVLFNALWQTGLLYLGFLAIGYRFL
                     KNVLNPAGAFYAVEHALNNFLYELAVTFLICSLFVYPCVPLETKALQFKPLCGLKNPT
                     TAVIGDSGTTYDEAFADLLTNQVKIPIGFAIIQNFMSSFTYSLMKVTGCTDSLQSIQG
                     DLVSTYLPQNIRKQALDFHRQCFIEARTQFNSEKHKASELDPMLKRYGGEDDLNWMGS
                     KILQKMYYSKLHARKPVPGFTFHQAPNRNLEKAANRGDIPPEQLPEDGYPSCQQWWSK
                     IKTDLVEVSNQASVFNKHLNYYAMLDRVRQYKVKHPKAWKADISAEDFIAKMLLQESR
                     DMQTSSVQSFMDNNNGKVASAITHSLVNVGQWTKSWTSTPLKREAIMQTLPVMQAFFM
                     FFLIILTPMVLSLSCYSTRALGSLCALFVMAIFLQYLWHLVGFLERSVLDPLGENDAI
                     SAMKNMAVMFYFVAPVLLLRLSSHFGGDAGAGLMDLVNGSDRQSESMTQSGMDVAKTG
                     VKIISKGIR"
     gene            complement(19054..19227)
                     /locus_tag="ACF2SP_000527"
     CDS             complement(19054..19227)
                     /locus_tag="ACF2SP_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358334.1"
                     /translation="MRKIFRLLAVVLDAIVGVAEDKPAKRYMSVYEAQDKLDNGLITI
                     REYNEAFERKDVL"
     gene            19242..19571
                     /locus_tag="ACF2SP_000528"
     CDS             19242..19571
                     /locus_tag="ACF2SP_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358335.1"
                     /translation="MIKHYIRSSIIVLIQTILPMIALLVIAPSFINSNLLAHWQSTVT
                     TIQPLFLGLHGALYLALILLWPRLISRLQNQHQLTTEQLSIALKARWYLLAIFVFIDA
                     LMIGSRL"
     gene            19568..21505
                     /gene="traD"
                     /locus_tag="ACF2SP_000529"
     CDS             19568..21505
                     /gene="traD"
                     /locus_tag="ACF2SP_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFD2358336.1"
                     /translation="MSNYPINNLLREPTEAWSAITCLSLALLAYTQPHLFLLTQTMGL
                     YAALALLIPGTYRAWQAIKVKRYHRRLLAMPTYALSTTQIPLSKNWLFLGKGFRWRPS
                     HTQKLHQIKQVKNEKFMQRSCCYQLARRFCQNHEHTKLAKWLNTNSKLNPFRPDPPVG
                     GSPFLHGVGEEDKPVYIPQDVRVGHTFVVGTTRVGKTRLASILINQDIRNGDAVIVVD
                     PKGDQDLVRDMIAACKVSGRIEDFKIVHLGFPEQSAQYNPLKNFDQISEVATRITDAI
                     SAEGEGKQFAAFAWKYVNIVAICLEEMNQPITYQTIAFYISRLEQLLMSYADKILPNH
                     YPNYHQEIDEIIEEHDSKIGKNNKTKSPMERHQAVIQYVKDHINKTIKSNNAEALHDQ
                     ILIDLYDAAIMDKHYYDKITASVGPVLSEINKSNASKILSSKAHPGEIELMDIIKNKK
                     VLYIGLDSLTNPNIAQAVGKAILSDLVSTAGKIYKEPNARYTLNLHCDELSEIIQDSF
                     VKILNKAGGAGFQVTAYAQTIQDMEVALGSKAKAEVSEGNFNTLIMLRVKNEETANLL
                     VKVLPQVGVVGHTQVSMVNDTPHGDDGIYFNTTNEDRVQTSSVPMIGVDDIVSLPKGQ
                     AFILTNGGDLFKVRLPLMKSI"
     gene            21536..21697
                     /locus_tag="ACF2SP_000530"
     CDS             21536..21697
                     /locus_tag="ACF2SP_000530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358337.1"
                     /translation="MTDFLKTNRLTISFTERSHFEDILQLRSDPDVQRFTIQRPQSHV
                     GILNDLWIL"
     gene            21717..21788
                     /locus_tag="ACF2SP_000531"
     CDS             21717..21788
                     /locus_tag="ACF2SP_000531"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358338.1"
                     /translation="MGYRFHKKHWGKGYETEVTKATC"
     gene            21875..22375
                     /locus_tag="ACF2SP_000532"
     CDS             21875..22375
                     /locus_tag="ACF2SP_000532"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358339.1"
                     /translation="MNTLKKEKKNRIMANLCIRRATLADAEAISHVIIQSLKKVNAKD
                     YSESVINSVIKNFSTEQIATKMKLRKIFVITEKDQIIGTASLEGNMVRTVFVLPSKQG
                     KKSGALLMHYLEEVARKENISCLTVSSSITAEGFYRKIGYNALRDEYYGEERTIIMEK
                     KLSELP"
     gene            22661..23971
                     /locus_tag="ACF2SP_000533"
     CDS             22661..23971
                     /locus_tag="ACF2SP_000533"
                     /inference="COORDINATES: protein motif:HMM:NF010309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase family
                     protein"
                     /protein_id="HFD2358340.1"
                     /translation="MNYPKEKIEPNPDAQQINECINLVTKVLGDNLLGVYLYGSALVG
                     GLQKYSDIDLFVVTNHATTLSEKNELTKHLLHISGRYMKSAKRPIEMTVVEKGAVNPW
                     HYPPRFDFQYGEWLRDSFEMGEIEPQNYEMPDLALIITQVLLKSQTLYGKKSEKILAP
                     VPYVDFINAMLQDLDRLAADIRDDTRNVLLTLARIWSTLETNKIRSKPDAADWVIVRL
                     PETHKAVMNRAKEICIGKKEECWEDIKESLKPCADLMLQNINELKSSVNLLDPCKRIQ
                     LMPPTFIIRLLKKEDIAPCAELFFNTVHHVNKKDYDQAQLDAWAPDAKLFIERFQVML
                     NRIAWVVEDNGQMVGFGDMTQEGYIDRLYVHKDYQRQGVARLIFSKWLDFAKAQDLKQ
                     LTTEASITAKAACEAIGFTLMEKQIVETNGVRFINYKMAYQVNK"
     gene            23968..24849
                     /locus_tag="ACF2SP_000534"
     CDS             23968..24849
                     /locus_tag="ACF2SP_000534"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358341.1"
                     /translation="MKELTFFKGYQQEDKKRAAFNRLAVNTFDLSFEEWYQSGYWRDK
                     YIPYTLFDGEHAIANVSVNIMDFSIFGHSQRAIQIGTVMTDEAYRNQGLSRRLMEKVF
                     EDWKADSHLIYLFANSSVWSFYPKLGFKSVKEYQYQRTITSRIHANFVKLDMDNKENR
                     EKLHNYAQNTCPFGKISMQENADLIMFYGITVYKNNVFYLPELDTIAIAEINGRQLHL
                     LDVFSKKEQNLNDVIHALSDETIDTVRLGFVPTDCSSYEIIPIDEKEKDEMLFVETEK
                     TALFDENQLMFPLLSHA"
     gene            24867..25325
                     /locus_tag="ACF2SP_000535"
     CDS             24867..25325
                     /locus_tag="ACF2SP_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358342.1"
                     /translation="MNEHNTLILLNELSEKINNLYGFVKIAGENFGEPAINSGPCGPF
                     ANAFYTIWNQKFTERVNIAFIMVKNSDECWHVLIRLPNGLLFDGGIGVHPDDRWDKNK
                     FDIVDMFQYDLQLLEKHSGGLNRKYPRYCPNFSINEVTHLITNCLNLIEE"
     gene            complement(25443..26225)
                     /locus_tag="ACF2SP_000536"
     CDS             complement(25443..26225)
                     /locus_tag="ACF2SP_000536"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2358343.1"
                     /translation="MLSLASELIQSNCASHQNIALVNFTHDITFGNNQISMIISEPNI
                     VSYYKRNQIPALCTDKTGRTLAEGVYLNKTLENSRKDCALLMPLLRKIGLKYKQNYGQ
                     NSVHILTKEHDCQHFYSLFFDLSENDFLQWVVNNGAFLNDFIVGYNKCASDLILEAKS
                     LEHRLTLPTTSDVVNLKKCQINNSEATLTLLHKELHLPIHLSKQQSRCLLLLVQGKSA
                     KEIASELLISYRTVEYYFDKIRKQLGCSSNKKLVADYGDQLY"
     gene            26412..27116
                     /locus_tag="ACF2SP_000537"
     CDS             26412..27116
                     /locus_tag="ACF2SP_000537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358344.1"
                     /translation="MSPKNSNTDKNEDPRKQAIESNPCGICRSAGIPNCKGHKGGAGG
                     GETSDEESEELIDEHLSSPMTAFKPSVNFIKSEVWRQPDSWESVFEFKNPDALITIKL
                     DFEQGILCLSSKERLPKDQQKAVDELFNAIKNEFNQFKKELANQGISVENMQLNHQGN
                     NLTLNINKPKLFDAFIQRLMDKNLLIAQAPTLQNDKQEKFSATPEKSMNTTLDAKQDY
                     NSTAPTPFDMTPKPKY"
     gene            27136..28968
                     /locus_tag="ACF2SP_000538"
     CDS             27136..28968
                     /locus_tag="ACF2SP_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2358345.1"
                     /translation="MKEAAIEFLIYKPNPEHPVPEEQRGVVVNPHLKQRFKQQFLPIG
                     VYLEGSDLRYYFFDQCDEAPEIYSSLDALKLDLPQELNTIFEKKPKLYIMGHASGGHY
                     GMGNCHGPSEQLFGDHFDHLLNSLKQTLPADHGEVFVTLEGCNTDNQLNATENQQEKT
                     FLERVSAKHREITFGGSRPWNAQEALTGARSLTPAAPITSMTGNVWKSGNSVIFHHGQ
                     YQIAVRKSLFASTQTAKELKINTIEYASAILGNNSRNELIAKIALNRDILTIQDLEKI
                     DGFPKLPIEEENVSSFIAQEKLILNKEQEHYLARVRGILERAEPIDQLTDRDILDLLL
                     GLKEPSVFMDHEYFLESILANKALLQLAIVSCGKVLVGGPSNDSLIDLLLKHGADINS
                     ADKEGMTALHYAVQNFYNYREEPLNLIKKLIDCGASLEVQNKKEQTPIDIAREHSQDG
                     RVTASDKLLSSLKHDHSPPTPLEVKQLVNRIFQMSQHKSDELLSLPIATREYFKCRDG
                     TLYIPEDVVEKLHGVLQSAKGNDELLSAFESELSAVISALERGIGTGEVFSEQDEFIP
                     KCFMDVNLRLTSVMDTLIELKQDMAASKVTEPEIVFADHRPESC"
     gene            complement(28997..29461)
                     /locus_tag="ACF2SP_000539"
     CDS             complement(28997..29461)
                     /locus_tag="ACF2SP_000539"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358346.1"
                     /translation="MNQIIRHRKANINDLPSIVFLLQDDELGRAREYIQPEMDQRYID
                     AFYKIECDPNQYLMIVENDVEIMGTCHLTIMPSLTFIGQTRMQIEAVRVFEKFRGQKI
                     GEWMINTAINYGKSQGASIIQLTTNKKRARAKKFYEKLGFEASHEGMKLYLD"
     gene            29609..30301
                     /locus_tag="ACF2SP_000540"
     CDS             29609..30301
                     /locus_tag="ACF2SP_000540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2358347.1"
                     /translation="MSIKKSLIMITGASSGIGGGIADIFSENGYPLLLISRNITSQQH
                     HQLPNTLYFQADVTDPISLSHAIKEAEIKFGPVECLINNAGTVKNGEFSKLSHHDHEQ
                     MVDVNVKGVINCIEAVLPGMQSRKTGTIINISSLADRKTRPKLATYAATKAAIKSLSE
                     SLRMANASLGIRVCNIAPAKVKTSLLQQSNLQENQIISVAELAKAVLWIYEQPQHICI
                     RDIVIAPTYYED"
     gene            30612..32810
                     /locus_tag="ACF2SP_000541"
     CDS             30612..32810
                     /locus_tag="ACF2SP_000541"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358348.1"
                     /translation="MYLFILLLGLTIVIARVILRTAHISNSRAEAEESIVLQIDEAPD
                     FVDEPEEKEISALQIISSLGTLVISPENRLRIAFATLLTAANTGLNFITPWLFAETLN
                     LLSGEDETTEIGGVEISSNMLITLLVSAYALSQVIPNLRDQTMVPVTAYNTKKILMMG
                     TQHLLKKSLDYHVNTPFSDQIYLIQKSFSLSNIGTPLLTQIAPTVVEIAIASSVLASR
                     YGYGVGAGLFAVMAAYTGYSALTAKPIIDAREMMLKTGSEAYQSFSDAIAQYKVMRDF
                     GQYEHTMKGVDAALTKMMHVEIDAHIKPQLISLGHISISRLGMLLATLYMGNGVKSGA
                     YSIQDFVILTGYLNSLSALLPAFGQAVNDLFASWPDLKFVFKELAIPDEVVDLNPETP
                     LPIEPESPPVIEFDNVTFSYTAKPGQAPVVLFKNLSFKIESGEKVIFVSESGAGKTTL
                     FNMLYGYYQPTEGTIKINGKNIAEVSLQSLQKNITLLGQSPNLFNGSIRENICYGSEN
                     PDEVSDETIWTLAEALNLRTFLESFENKLDTNVGENGKTLSGGQQQKVAILRGFMKKT
                     SIRLMDEITAPFDSQSAKSVLESIDKLSTSVTTMMITHKLTEGQNASKIIVLAEGRVI
                     DEGSHATLLQRCNLYQHLWNAYIAQNETLSSSSTARMLASLGGPQTSETMILEHNDPS
                     MAFVQNEETNDFKHCADQIKTQYTLHASSSRDTEERHFDSSPLEITPKPQ"
     gene            complement(32974..34758)
                     /locus_tag="ACF2SP_000542"
     CDS             complement(32974..34758)
                     /locus_tag="ACF2SP_000542"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358349.1"
                     /translation="MNTTQKLTLPRYIFAMLKPYRRLVLLMTLIGFFWAFITTFMPWT
                     LKMIIDHAVSFSGDKQQLFKSVMPWIGCYLALWFLLCTSMRFLDWIRLKLFPVLREDI
                     CMSMFRWLNQHSHRYFQNNFAGSLINKINDMQSATINILNIVDDVYTQFLGFLVAIGV
                     LLYLYPIFALILISWVIVFLGITFLFLRPIKNASQHYAESITQFVGNMVDSVSNVMNV
                     RLFDRFTTENQYIQNSVKEAVKRDQALQAKIIGMRVLWDISIICLLGLNLIVLLKMYS
                     INKVTIGDFTFVTSLSISILWNLWFVAGQFVSFTEQIGRCKQALSIIVEPHEIVDAAD
                     ALPLKLNRGEIRFDNVTFHYDEGARLFDNKSVIIDGGQKVGLVGFSGSGKSSFVNLIL
                     RLFEVESGDILIDEQNINHVTQQSLREHIALIPQDITLFHRNLLENIRFGDPTASDEA
                     VIDAAKKAHCHEFISELPEAYQSMVGERGIKLSGGQRQRIAIARAILKNAPILILDEA
                     TSALDSVTEQYIQESLYHLMQNKTTIVIAHRLSTLAKMDRILVFDKGRIIEDGTHDSL
                     LVLKGHYARMWQMQAGGFLPDKERAGTE"
     gene            35177..36589
                     /locus_tag="ACF2SP_000543"
     CDS             35177..36589
                     /locus_tag="ACF2SP_000543"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFD2358350.1"
                     /translation="MPKITGIDRKLIESTMRKAHIPGVSIAYRDGKSTIPSTTNIGTT
                     DTRDTSILSKVEDDTVFGAASLSKPVFAYLVLKLIDKGVLSGPGKSAGSGLDRPLHEV
                     FSLEKFFHEHDKQLSEIDIERAKAITPRMLLSHTSGLGIDASATLSFAPGTEYAYSGT
                     GLEYLQRVIKEQTGKSLCTLAQEHVFGELALDMKMSSFVPPGTSEDKADAANSLYTTA
                     SDYTKLMIAWMQDPSPIMQQAFVRQVSLTQDNQRLPGESNPAAEHVDMKVKECLAWGL
                     GLGLELDETGKAVKAFHTGDMNQFRAQTALDLEKKSCIVYFANANSDLQANGHVLAPL
                     IIKPKIPIDYAHTWFYSKFPFALKPDQLTEGSRFGLRVQGKDKKADSDAVMTAFMPGG
                     APTHVLNPDASDGSSYAVMRALMPTHSPTPALISPELEEQSSVESPPTTAIEEEIQAE
                     TDRPSSPSPFKLTPKPSGYS"
     gene            complement(36793..37509)
                     /locus_tag="ACF2SP_000544"
     CDS             complement(36793..37509)
                     /locus_tag="ACF2SP_000544"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2358351.1"
                     /translation="MNISNEFVFSRYKKGIVLLPPETMTETAHLSVENILKQPFSIYF
                     VKGNDCSLGKINSTCAQNCGFDSEFSAIGKIASDFLNKESAHFAVAVDKTVMAQETVN
                     ICEHMLEHNEGTSYQYLTIKSPWYQQQNVVGVFGCSIMLGLHPLAESLQCIAELGLLS
                     ANVFKTVVAKDRASLSKQELSLAKLLITGLTAKEMAKRVNLSHRTVETYLINIKAKLQ
                     CKNKTELVIKLADLLGYSRY"
BASE COUNT        12052 a         7793 c         7239 g        10568 t
ORIGIN      
        1 agatataaca aaatacagta aatggaaaca ccttatagga aaggagcaag agtatatatg
       61 gagatcgggg ttaaaacatg actgttcaaa agttatggaa cttgaaccta ttgatggaag
      121 ttataaaaat ggctttgggg aaatcgtaga tcttgaagac aattatattt atcccttcct
      181 taagagttct gatattggta attccagaat agattttaat aggaaaactg ttattgttac
      241 tcagaaattt gtaggtgaag acacctctat ggttaaaaac attgcaccta aaatatggca
      301 gtatttggag tcatattcta atttgttaaa tgggagaaaa agctcaattt ataaaggtaa
      361 gcccgcatat tcaatttttg gcgtcggaga ttacactttt aaatcttgga aaatagctat
      421 atctggctta tataaaaaat taaacttttg cttaattcct cctatgaaag gaaagcctgt
      481 tatatttgat gatacagtaa attttttatc ttttgattcg gaaaatgaag caagatttat
      541 ctataaatta attacttcaa aaattgcaga agagttttac agctcaatga ttttttggga
      601 tgaaaaacgg cctattacta cagacatatt gtaagcggtt aattaacccc atcttttaaa
      661 tcaaaagcta atttcagata cttgctgcca ttgaataacg caggagtatt taaatgaaag
      721 tatcatcaaa cgcctataaa caaggcttct ggaaagaaca ttcagaatcg tggaaagcaa
      781 gcgggttaac gcagcaggct tattgtgaac aagaaggagt cagctaccgc agctttgttt
      841 atcagcacaa tcggttagcg agtcaatcaa aaaaagcgtc gttaaagttt attgaagcga
      901 aaccagggtc agtagctatt aataatcaag cagcaggatt gcaattgatg cttcccaatg
      961 gcattcgtat aggcatcggc gctgaaatca atcctgaact gctccaaaca gtcttaagtg
     1021 tggcaggagc agtccaatgc tgaagttgaa tgaagacacg cgtatttttg tggcggataa
     1081 accggttgat tgcaggaaag ctatcaatgg attagtatct ttggttattg agcagtttga
     1141 acagcctgtt aacgatggtt ctgtctatgt gttttataat ggttcttgcg accgtgtaaa
     1201 gtgcttgttt tgggataaaa acggctttgt tctttatcat aagcgtctgg agcgaggtcg
     1261 attcaagatg aagaagactg cagatggcgc ttatgtgatt agccaaaatc aattggattg
     1321 gctgttggca ggccttgatt ttcagctgat gaatgaattc aagacacttg atttcaccca
     1381 gtatttttaa tccaaaatga ctattttagt ataaatgtgc ctatttgttt ggtataattc
     1441 accctataaa aacaagaaca taaccgatta agatgatttc aatttctgct caaacattag
     1501 aacaattaca gcaagaaaat gctcgactat tggcattgat tgggcggcgt gataatacaa
     1561 ttcaaaattt gcaacaccaa cttcacttat tccgcacggc acgtttcggc cgaaaaagtg
     1621 aaaaaggcgt agtgcccgag caaatggcac ttcaatttga tgaagtctat gttgcttctg
     1681 aggttaatgc atcagaagag ggctcagaaa ctcaaaccga aacgattacc tacactcgct
     1741 ctaaaaaaag cactggtcgc aaaccgcttc ctaaatcgtt accttatgtc gaacagattc
     1801 atgacttaag tgatgacgaa aaacaatgtg cttgtggttg tgctttaacc catattaacg
     1861 atgaagtgac cgagcaattg gatgttgttc cccagatgac ctttcgcgtg gttcacatcc
     1921 gcaaaaaata cgcctgcaaa ggatgcagcg ataccattcg tttagccaaa ttaccaaaac
     1981 aacccattga taaagccatt gccacgcccg gattattagc ggcggtgatt aacagcaagt
     2041 ttaatcacca tatgcctttg tatcgccagg aggcgatgtt tacgtcagcc ggcatctcag
     2101 tgactcgcgc aaccctcagt aactggctca ttaaatcagc agatttaatg actccattgg
     2161 tgaaattgat ggaggcagat attaatgatt acgatattgc ctatgctgat gaaacctctt
     2221 tacaagtctt aaaagaaaag gatcgggcgc ccacacaaaa atcattcatg tggctctttc
     2281 ttggtggccc accggataag cgcgccgtgg tgtatcaata ccatcctaca cgtgggcatc
     2341 agataccaca tgacttcttt gctgatttta aagggtatgt ccatgccgat tgctatcaag
     2401 cctacgtgac tttaggacaa caagagcaca tccatcatgt agcctgctgg gctcatgctc
     2461 gacgctattt tgtcgatgtg gctaaagccc ataaaaaaca gggtgtagct catcaaatag
     2521 ttgctttaat cggcaaactc tatcagatag aacgcgagct caaagataaa gccgcaacac
     2581 ccgcgctagt ctttatggcg cgtgttaaaa aatccaggcc catagtgctt cagattaaag
     2641 cactacttga tgacgcgcaa cttaaagtac cccctaaaag tcctttgggg gctgcgctct
     2701 tttattccct gacacactgg gaagcgctca atatcttctt attcgatggc cgtttggagc
     2761 tcgataataa cctcggtgaa cgtaccatta aacctttcgt tatcggtcga aaaaactggc
     2821 tctttcatgg caatgacctc ggcgccaaag ccggtagtat cctcttctcg cttattgaaa
     2881 cctgtaagca gaataaaatc gaaacttttt cctggtttaa atacgtcctt actcatattc
     2941 gccaggccag cactctagaa caactggaga accttcttcc ttataacatt gaccactcat
     3001 tactaactga tatgcgtagc ttacccaatc tcattatgcc tgagaagagt gaggttaatt
     3061 aaccgtttac gacatattaa gaagattatc cttaagaaaa ctggcagatg agttaaagct
     3121 tagcaatgaa tatcagggtg ttgtaaataa atcatttatt attaaaaatc agattgtact
     3181 tgaggatgct taaatggaac gatagttatt actctatgtt tatgatattg aggcccctct
     3241 taagatctta gaggggctga ttataaaaaa ttcagtaatc aatagctaat aaaaaaatgt
     3301 gtggtacaaa ctggtaatta agccctaaat gttatttttg gataatgaat ccattcccca
     3361 ctcggattat ccgtcaaaca gagcaaccaa gaagcggcaa ctctcaactc cttggacaaa
     3421 atcatagcct cctccgaccc aggactccta gttccctgct cccaatttcc aatcctagcc
     3481 gcccctaatc ccgtcctttc agccaagact ttaatcgtca actcattcgc ccttctagcc
     3541 tgcgtaatcc tactgccaat aatttttttc atgttcattt aggttcattt gctatcaaac
     3601 ggaaataccc cattgactct aaaaagcgcc tatttacaat gggttatcaa caacacattt
     3661 cgtgttattg attgatacta attgaaatca tttgatagtg caagtgctgt ttttagcggt
     3721 gcttattaag gatgaaatag agaaatggga tttttgtttg ttaataccga tgaattatct
     3781 gcactgatgg gattgcctta tattcagcaa tcagcctatt tgctgggaat tcgtccgtat
     3841 atggatcgca atacctttct ggtaggcgtt aagcgtaaaa tcagctacca acagcttgct
     3901 gaagcccttt acgttgaacc gcaccaaggt tttgaatatt caggtagtcc tagccgtccg
     3961 caattacgcc gtatcatcta tgcgcttgaa cgcgctgggt tggtggagat caaatctatt
     4021 ggcaaacaac tcattttaaa atgcttatta gccaattcgg atttgtctgc tcaaaataaa
     4081 cccgtcacaa acccgacaca tcaacccgac acaaacccga acgacaaaaa caatattaaa
     4141 tcatcaagtt acgatgataa aaatcaaaaa cccagcacag cgcaaaacac aaaacccgac
     4201 acacctcata actccaaaga tcgttttatt tttttaaagc agcaatttga aaaattttgg
     4261 tctctttatc ctttaaaaca agaccgaact aaagcctggc gtgagtttgg ttatctgcaa
     4321 ccagatgaac agatggttga acaaatactg acaggcctgc aagcccaaat taataaccga
     4381 gaggaaatgg agtttattgg tgagtggcta ccgaactgga aatatccagc tacctggctt
     4441 tcacgcaaat gctggaacga tgaattactt accttaaaaa cccaagagga gcagcaaaat
     4501 gaagccaatc aagtgcgttc tcgaaaaaaa tcagcaacag atatccttac tgaatcctgc
     4561 aaagatgcaa acttcgattt cgactttgat gaaccatcag actcaggaaa caacatcctc
     4621 tcattcgacc acaccagagt acgataagcg aatggatacg ctgtttatgc gacttgcagc
     4681 aatttatggc tatttgtggg tctccactta tagcaaccct agttttctcg aactctcaaa
     4741 acgtgaatgg tcagaaagct taaacgagtt tgacaaccaa gtacttaaac ttgctttaga
     4801 acaaatcaag aagaagttgg ctgttcctcc ttgtcttccg attttttatg agtactgcac
     4861 taacatccaa aaaggcatcc aagcccgtca agaagcgatc aaacaaaaaa ctgaaccgca
     4921 tatccctacc gatccccagg tcgttcaact gcatatcaac caaatgaaag actacctctt
     4981 caaagaagct aaggagaaaa catcatgctg accttaatcc gtcgtatcgg cgaagcaatt
     5041 tacattgata aaggaagaat caaagtgcat ttaatctctg aaaacgaggg gctaattagg
     5101 ctaggtatag aagcacctaa gcatgttgat attgaacgta aagaagtctt tgtccgcaaa
     5161 gcagtggcac aacatgaaca agcgcaggca cttagaaacc aatcaggagg cgatgatgct
     5221 taagaaaact atggtattca gcctagtgtt atgtggtctg caaactcatg cagccaatat
     5281 cacccaagta agccgctatg ccacggttaa aaatcaaccg ttagcagccc aaattaaccc
     5341 catgaaaacc gtacaacaga ttcattttcc tgcctccatt caaaccatag gggaggcagt
     5401 ggattactgg ttaggttatt ccggttatca ccttgcaccc aaggaaaaac aaagtgaaag
     5461 cctgcaacaa gtctttcaac aaccattacc acaagtcagt agaaatcttg gaccactaac
     5521 cattgaacga ggtctgacgg tattggtcgg gcaacaactg ttcactctca aacaagacga
     5581 tttgttaagg gaaatcaatt ttagtctgat tgcaaggagc gcaagatgaa aaagtatctg
     5641 gatgttaaat ggcttagcct gcttgtttta aatgttttat taattacttt tgtatgtgta
     5701 aaaaatcaat cgcctgcaaa tcataatgag ctaaatatcc ttggcgaaca actcacatct
     5761 attcaaacgc aattaaaaca acctgtagaa caacctgatt tatcgctaat tacccaaaac
     5821 ctcaaccagt taaataactt cgttcagcaa ctgaaaaata aagacgatca tcaattaggt
     5881 gaaatattca ctacagaaca aggtgcaata aaaaaacaac tcgaaggtat tacggattta
     5941 ttacgccact tagatgagaa aaagaaacct gtgaaaatgc tctctgcaag tcaactgccc
     6001 tttaaggtat taagcataga cagcatccaa gaaatacccg ttgcatccct tgcttaccac
     6061 tacaaaaccc aagcattgga aaaaggcgat acgttagctg gttggacagt ttttgaaatc
     6121 gactttgcca aacaaatcat agaatttgaa aacgcagata aagcccatgt actggttcgc
     6181 ttaaatcaac atgaggtgag ccatgcttaa gttatctact ctagccatgg gattagtact
     6241 tgggcaacag gccatcgctg gtttgtatat ccctggcatt gccacctcac ccatgcaaca
     6301 agccgacaat gccttggcaa aagcaggcct tactgagttt catgatgaag tcatcgaaga
     6361 aaaagatttc caactaaatg acgctcaaaa gcaagaagcg aaagtctggg gattaaatga
     6421 aaaggaagaa aaacgctatc tgcaattaat gcagagccgg agtggcgtgt attacaaaga
     6481 cttgcgaatg acaccgattg atatccttgg acttaacgcc aaagatgatg tggagcgtga
     6541 acattttgcc agcctggctg tccgccagga agcgcaaaag gtggcgcaaa atatcgcctg
     6601 gaacaatgcc ttttacaaag cctataacga actgtttaaa gatgtacccg tggttggtga
     6661 ttttgatccc tccccttact caccctatgc acatcaacct attcaattaa aaacaggtga
     6721 aacactctat ttatttgtaa gacctgacga tgctgttacc acgattgttc tgcaactggt
     6781 tgacgcgatt aaccgcactc ccaatacaaa acttaacctg ttgtttttgg atatggacaa
     6841 taacgccata cagttatggg cgaaccgaca tcaaatccct attcaattag tgaccagcca
     6901 acaagtcacc ctaacctctg gcaaccaaca atttgaaagc ttaacgcttg ccaagaaaca
     6961 aaccccatta ctgttaatag ccaacggtaa aacctcgcaa gtcgtcgatt tagggagatt
     7021 ttaatgatta ggattttgtt cttactcttg gtcacgttga atgttcatag catgaacgtc
     7081 attcctttaa ccctgtcaca aagtgatacg tcattaaaat taaatctgtc cagcccaata
     7141 ggcgtaccag ccaaaagcaa agccaccgca ggcaaagtca gccgtaaaac attggatacc
     7201 agtttattaa acatgccttt gtttgtcata ggtgcagata aagcgtcgat tcaatggtta
     7261 gaacagcatc aagaagaatt aaaatccatg caagccacag gattcattac caacgtcaat
     7321 gactttgaat ctattgtcgc tttgcaagaa aaattcaaac taccgttatt accagttaat
     7381 gttgatccat tgcttgcata ccttcatgaa caacattacc ccctaatcat tgctgagggg
     7441 gctgtatggc agtaaagcaa tattcaaggc aactgaccat gcaattaacc atagtgctat
     7501 tcttaggttg gttggcgatt attatctggg ctttaagtca atggcttctg catggctact
     7561 catttgcttt tgaaaaagtg aatacccttg taaacactca aagagaagcc atcacccctg
     7621 tttatcaagg ctcacttttg gcagttcttc ctttgaattc taaaccagat tgggcattcg
     7681 ctatccccta cttaaacaaa ctggccatca atgaccttgc caaggactta atcgaaaaat
     7741 cacaacaatt cttgcagatc cttttattaa gcagccagtg ccttttaatc aaactcatta
     7801 tcctattcgc cgccctgccc ttatttgctt taaccatgat tgcaggcctg gttgacggcc
     7861 taaatcaaag agcaatacgc accgcttgtt taggtcgtga atcctcatat gtgttccaca
     7921 ggcttaatca ctatttaaaa aagggactgg tgattttact catgctgtgg cttgccctgc
     7981 ccgttagcat cacccctgct tatgtctttg tgcctgtcag tttgttgatg ggattaatgg
     8041 tagccatgac ggccagccgc tttaagaaat acttataggg agaaaccatg aaacgaatat
     8101 tatgcctgac cttaatgata ataagcctgc aatgttcagc gaataaccct gatttgaatg
     8161 aaacactggt acgcattatt aatcagatta acgccatcat gcctttgctt gatgaagcgc
     8221 aaacacagat aacaccaaat gctcgtatcc aattgcaaat agaaggattt gaagatctcc
     8281 aaggccagca tcacgccgga ttgagagaag acttgctaaa catccgcaac gggctgattg
     8341 actatatcaa ccagcccatc attgcccctc gaaaaattaa accattagaa atggattttg
     8401 tgaggaaacc ctaatgagta aagacgaaat agcgagaatg tttgcttcaa cattcaaaca
     8461 agccagcaac aacatctcag cccaagtctt ttccagcagt atccgtttta ttgccataag
     8521 cctggtgatt ttagcggtga tgtggtgcgt caaccacatg atgggatccg aggaaaaaca
     8581 gcaacaagga tacttgctga acttcggttc tcgaatggtc aggcttgtca ttggcctgat
     8641 gattttcatt ttagtgttat tcgttaagga gacaacatga aaacaacgct tcgcacgctc
     8701 tacctgggag ctgtcaatct tttttatgcg cctgtgttac tggcagataa ctggttccct
     8761 aaaatttctg atgcggacaa catcggtgac ggttccaaaa gcatgatgtc ggtaacaggc
     8821 aattacgtca aacagggtct tggcttattg ctgttcatca ccgcagtcgt gacctttatc
     8881 aagttcatta ccactgtgca acacggtatt gaagaatcca aaaagaacga aggttccatg
     8941 gtggccttcg gtacttttgc cgtaatgggg attacctatt tagccattag catcgcttct
     9001 ggttatgtgg gttacaccat gattaccaag ttcaaaatat aggaggtgaa attatgagtc
     9061 agccttcttc gcgtcatcta tcacatgact tcccagcctg gaaaggctta agtctgcgtg
     9121 aattgttttg gattgtcatc actacaacac cagtaaccac cctgcttttt atccttttag
     9181 gaacaatggc aggatatcca ctggccgctg gctgcatcgg ttttgtgatt ggattcatcc
     9241 tctccattac cgtgtggcct aaaggagtcg cccgtatcaa agcaggcaaa ccttatggtt
     9301 atgtgatgaa gaaaaccatt caatggatgg tgcgattaag gctcaaacac tcgccctgga
     9361 ttcattatca aggacagtgg caaaagaata aatctgttgg agagcctcat gtttgaatat
     9421 tggaaaaaga tcgacagcct acagcatcat aaccgtttat tactggcttt tatcggcctg
     9481 ttatcagcca tcgttttggc cttaatcatt aacctcatga ccatgcccaa acgctatgag
     9541 ttttggctaa gtcctaatat ggcggccaat ggcggtttaa tgaaagcaag cgatatccct
     9601 aatgaatatg tgcaaggatt tgtgtcctca ttaataccaa cgctatatac ctggtctaat
     9661 aaaggcaaag aagaatttaa ccaaaatatc aaagcctttc attattattt tacctctcgt
     9721 catgaacaat tactcagaga aacgctggga gcttataaaa acgcgcagct ctttgaccgc
     9781 agtcaagtag ccagtctcta tcgtttcatg gagcctcaag atattagaaa aattggtccc
     9841 gatacctggg aagtgaaatt aatcctgcgc attactcaaa gactgaaaga caacagtggt
     9901 atggtgattg ccgacaaggt agtggcctat cacctgcgtg tggtcaaact caatttgtct
     9961 cgtttacaca accctttcca actcgctctc gatggctaca cccgtcctga agaacgttta
    10021 caagatttat tggtcgacac ggaggaatct catgaaactc attaaacaca tgttattaat
    10081 aattggcttt atgctcacgc aagtaagcta cgctctgcaa agtgaacatc tggtttggga
    10141 aaaagtccct ttaacgattg aattacctat caataaagaa aggctcgttc aatttcccca
    10201 ggccattaaa attattgatc aacaactcag taccaacctc gatattctga aagtaaaagg
    10261 cagtctgtat ttaaaggcta aagacacctt caacgatgca cgtttgattg tgcaactctt
    10321 acccgaaggt gaagtcatca tactgaacct taaagccaat gagaaagctg ttaataccac
    10381 acctattgaa atcctcattg atgatcccaa agtcagccat cagtcaaatg caagccaata
    10441 tgaatacaat gctatacagc tcacccgttt tgccattcaa gccttgtact cgccagagcg
    10501 tgtcagagat attccagagg gtatttatcg ctcgcccatg caaaccaata aaaccatccc
    10561 cttattctat ggagcaagca ttgaagctca ccctctggct tcatggcgtg gtggtagcct
    10621 ctatgtaacc gcggtcgatt taaaaaacct gctaaatcaa cctgtcaaac tgcaattttc
    10681 aaaactcatg gggcattggc aaaccgcaag tttctacccc aaaagtgaat tgcctccgcg
    10741 aaatcaacac gaaagtacaa cagtctttgt ggtttctgac caaccctttg ccgcagcact
    10801 tactcaacat gcgaggtaca gccgatgaac aaaaacaaag gtattaaaat cttagctgga
    10861 tcggtggtgt tcgcagtcgt catgattcta atcaatagcc gtcatagcgc gcctatccat
    10921 gatgacacac ctaatcccaa caattttcat gaagccatta ccagccaatt caacgacaac
    10981 atccgtgatg tgtctgcaag actccttgaa acagaaaaga agctggaaca aatgcacaag
    11041 gagaataaat cgctgcaaaa acaattaaag caacccatac aagaagcaag tcatacgcta
    11101 caggatgaac tgcaaacact gaaagaaaag ctctcagcac ttaccgatca tcaaacccaa
    11161 tcttatccca tggaaggcaa tcaagctcct gtatctggcc aaatcaaaga tctggatgga
    11221 ttgttggata aagcccctat tgagagcaaa atctttgcag accaagactc atccaccaaa
    11281 gaagaagcgc caaccaaaac acccttctat accattccgg caggtagtga ttttagcaag
    11341 gttacgcttt tgtctgccct aatgggtgaa gtacctgttg agggcaaact aatgcagccc
    11401 ttatttcctt ttaccgcaat ggtcagccgt ggcgatttaa tgacagccaa tgggatgcaa
    11461 ttgccggaag aaatcattgg tatgaaaatc agcggttatt ccattggcgt aggctctttc
    11521 ctcgataaca tcagctgcgt ccgtgcttat gtcacctcag ctttatttgt ctttgaagat
    11581 ggacatttcg tcagtgctgg tcaggaacaa atgaaaagct ctgcggagct ggtgaacaat
    11641 gacagcattg gttatctaac tactcaattc ggcaatcctt gcattaaagg acagtatatc
    11701 actaatgccc ccagagtttt agccgctttc atggctgctg gcggtattca aggggcaggt
    11761 aatgctttat ccaagtggca aatgagttat caagcagaag gcggttctgc tattgccaca
    11821 cctactggcg atttagcaca ttttgcagca ggtggagcta tcaacgaggg cagccaaaaa
    11881 attactgatt ggcttgagaa aagaatacaa ggcagtttcg atatggtttt tgttcctgcc
    11941 agcatccgca gccactcagg ctttatacca aatcaattca gtcttcattt tagtcaaacc
    12001 atagccattg ataaagaaca ccaagggagg gtattagatt atggttacca tcaacaaaag
    12061 aactttgaca atgctttgcg ctagtctggc tttgagcgct tgctcaaccg ccaatatatc
    12121 gcaaagtgcc attcctgagc agggattaac agtcagtcag ctctatcagc aaagcatgca
    12181 acagcctata caaaaggctg ttgttcaaca ggtacggttt gaggattcag gcgaagaatt
    12241 taagcagctg ccgaatcccg aagtgcctat tcatattttt gcgcatgtgg ctcagctcgg
    12301 cgatgaacaa atcatcaaac cagcctatac cacacgcttt tttctttaca agcaaaatca
    12361 atacgccctg gcatcagaac tgtattaagg aggcatgatg aaacgattat ctaactggtt
    12421 gcttggggaa atgaatcccc aggctataaa agaacaaacc attaaaaagc aatacgccaa
    12481 tcctttacct tcttttgctg acaggctggc cattgttgat ttcaacgaac aagaacaagt
    12541 atttttattt gctgatggaa aaagtttagg ttcagggttt gagctaggtg atattcaagc
    12601 cgaagcagct tctcctgaat acttgcaagc tgtctttaat aaaatccgcg atacctttgc
    12661 aacggtagtt ccccttcatg cgatagatcc ttgggtcatg caaatgtttg ttcaagatga
    12721 atattgcctt gatcccgtta ttaatcatat taaatccaaa atcccacaac aactcgttga
    12781 aaatccacta acgcaagatt acttacaacg cttacaagat ctctacaaca aaatgaccag
    12841 gccagaaggt ttatttctcg atccgaaaac aggtgcgcct tatcgtggcc gcagacgaag
    12901 aatccgagta ttgttttata gacaattaca tcaaaccact ctaccccgcg agcaaattct
    12961 tctcgaacat caagaagtcg tcagtcagat tgaaaccaaa ctacgttcgc caggcctcga
    13021 aatcaaacgt cttacgggtc aggattatta tcaatggtgg atacgctggt ttaatcctaa
    13081 atcagccgat gagatcttgg aacaatatcc ctaccctaat tccatacccg caggttttaa
    13141 tctggcgcaa aatatattct tttccccgcc tgagagcgat gagcaaggat ttatttttga
    13201 aggccgaaaa caaagaatcc tgtatgttga tagtttaaaa gaagcgccca ttattggttt
    13261 agtctcccga gaaagacaac aagccaatcc aaagcaccgc tatgcgcttt tggatacctt
    13321 gccagaagga agcatctaca ccattcaagt cgtctttagc catgatgaaa atcttgattc
    13381 ccatttaaca cgattagaaa agggaattgt gggaactagc ctaaaacctc aagaagtcag
    13441 agatgatata aaaacagccc gtaatgaatt aagtactggc aatcgcctgt tctgggttaa
    13501 tcaggccgtt ttttatcaag ccgaagatga gcaacaagct caggttatag aaaaaaacct
    13561 acatagcatt ttcaacgatg ccaagatgcc cttaattcat tccatctatg atatccaccc
    13621 attaaattcc tggctcaatg ctctaccgtt taatttcgat ccccactatg cccgtaagta
    13681 cttatgcttt gaccgtctga tgtatgccac agagcttgcg gctttacttc ctgtttatgg
    13741 acgaaaccaa ggcgcaagac atttaccctg ctttcctttt ttcaaccgct taggtgaacc
    13801 tgttttcttt gacttacttc accatgattt tattagccaa aattcgcatt gcgcgatttt
    13861 cgccaactct ggtggtggta agtcagtttt aacaggttgg atgattcaat cactgctcgc
    13921 catgaaaaac gcccgtgtag tactttttga aatggggaac tcgtttgacc gaatgctcat
    13981 tcatgccaaa gcccatggca aaaaaaccaa acagttatta ttgagcaata aaaaagaaga
    14041 agctgttcca ttaaatccct tttgcgaagc gtataaagcc attccagaaa ttacagataa
    14101 tttaagcgca gaacaagctg ctttaatagc tcaaaaaatt atggatctta aagatcctgc
    14161 tcactcagaa gatttggctt gtggtgatgg ttctcgcgca tatcttgcag agcttgcctt
    14221 agctttgcgt accatgatta cggaagccaa tgccctggaa gaagaacaat ttaccctggc
    14281 tgatgaaaca ctgttaatcg aagtattaat tgatgccatc ctaacttcat ttaacaatgg
    14341 cataccacaa atgctgactg aacatattgt gagtgcattt caacgacgat tggataaaga
    14401 aacggtggcg cgtaagaaag accggattct ggatatgcac gaccgattaa acagttatgt
    14461 cattaacagc gcgaaatccc gcttctttaa tgtgccaact gagcctttag gtgattttga
    14521 tatctttcat attgatattt ccgccattaa agatgatacc ggaaaactgg ctttggtcat
    14581 ggtgtctttg ttgccaagaa tactggccat ggctgaggct actcaaaacg ataatagacc
    14641 aacctttctt tttattgatg agtcccacct gcaatttcaa attccttctg tggtgacagt
    14701 gtgtctactg gttgccaaag tagccagaaa gctcgggctt tggctggttg cgattaccca
    14761 aaacgtcacc gacatgaatt ctgaaaaggc tgcaaagatt ttatcgttaa ttgaaacctg
    14821 gattttatta ggattggatg aaaaagaaat cactgatgtg aaacgcttta aatccttaac
    14881 accgcagcag gaaacattaa tcagagatat tgattcgcaa aaaggtttgt atgcagaagc
    14941 agttttatta gggtcacgct accaaggatt atttcgcgtc attccacccc gctacctgct
    15001 tgccctctta atgactgaga aatctgaaaa agcacaacgc caccttctgg agaaagaaca
    15061 tgatgtcctg gtcgccgctg aaatcatggc taaaaaactc gaacaaccaa aaacaactac
    15121 taataacagg gcttattttt atgactaata ctgcctttgc tttagaaagc acaagcccgc
    15181 ctcatcctgt gaataccttc acaatcgcca cacgggtttt gcaaaagctg tttcataaca
    15241 gccattacaa agtcattggc tcttgcactt ggacggtggg acgtttaccg ccaagacttg
    15301 aagtcacccc tgccattgaa cagtttttac ctgatctaat aattacggtc agtaataaac
    15361 cagaagaaaa tccctggctt gaagcgcgaa ccctttatga aaataaagct gcaagaacga
    15421 tgtatcaaca agcctataag gctgcaactg gttcagcgtt gggatttggt aatgacagcg
    15481 gtcaaaccac agaccttcat atcaatgatg agcgcacgcg cattgttgat gtgattggca
    15541 gtcctgctgc attttaccgt atcccctatc tatcacatag gccggaaact ggttttggcg
    15601 ttccttatta tttggccgaa gctgatgcag tcatggatag aacagaagca gctgaattgc
    15661 tgtatatggg aacgcatcct catctgttaa tcaaccatga aatcggcaca ttgacacaac
    15721 attggggttc tgaaattcca agattgatgc gcgtaacgca accctataac tacagggctt
    15781 cagttgttgc agccatgcac gcggttgata ttgtcactaa taaaaatccg ttgcatgtca
    15841 ccaaatcaac cagtaattcc tgcggtaaaa actgtgtggt ggctaatgcc atttacgacc
    15901 cacaaaacac gaaagtcatc tggcaggaaa tttatccgtt aaatcgcaat attcacccag
    15961 gcaacgcaca ggatttcggc attgaagacg aaaaagccgg aaatggtaat tatgtgtttg
    16021 tcctttggcg caaataccgc ggctgtattc agcataaagg caaactcgtg aactttctta
    16081 cttttcccaa ggttgggcaa cctcaaaaac gataggacat catcatgaag aaatctctat
    16141 tactagcttt aatcgttcca acactgaccc atgctggcag ctttatgccc agccaatcgg
    16201 attactatta tgaattagga ggaacatcca atttatttat tccacccgtt aataaagacc
    16261 aaacactggt tattggtgca gatattgatg gccgtatggg attctcttgt agcggtttta
    16321 atcctgtcgt atccattacc aatacctttc aggatttgaa aaaatcagcg ttaaatatcc
    16381 ctggcggtgt cattgataac ctcaaaggct ctgtcgcagg attcccactt tataagctac
    16441 agcagtccat gcctgcttta tacaatgttc tacaaaatgc cgcatccagc gcacaaaatg
    16501 aatttaccct caaggttaag gattgccaag aagtcaaaca agctctggaa caaaaccaat
    16561 cgccaatgga aggcatatta tcagtatccg atagtcaagg atggctggac gctgctaaac
    16621 gcgctaaaaa ggaaaacgtc gatgtgactg agacatcgaa aagcatcgcc caaaaacgag
    16681 atgaatatgg cctgccctgg ataggccacg ataaaggcaa tgctggcggt aaatttcaac
    16741 gtcctattaa ggtcatcaac gatgtggtca ttgctggcta caacatcctt ttagagagaa
    16801 aacctttaga ttctttggaa aagccaagta ccaaaatccc tatggtacaa agctggccaa
    16861 cgcctaccga tgctgctaat tgggcagtta aggttcttgg tgacatccag gtcagtagta
    16921 gtgagaacaa acaaagccat gatgctaaag caggaattgg tttgtcagct ttattgcaaa
    16981 gctgtgcgaa cgccaatact tgtagccaga acattgccaa agccttatgg aatttggtca
    17041 atggtacatg gaaactgact gaggaaaatc tgcataaagt cagtgcttcc aatttactga
    17101 ttaccgatga agtcattacc accattcagc atctaccgcg cgaggagcaa atgctcactg
    17161 tctcaaaact ggcagaagaa atcgctgttc aaaatatgtt ggataaagcg ttgatgatgc
    17221 gccgcatctt gcaggcagga ttacaagtgc aagaagtaca aaatttaaaa cctgcgatga
    17281 atatggtctt gtttgccctt aaaaaattag atgatgacat tcactcgtta tcttttgaaa
    17341 acgatgtccg caaaaaaatg atgactgaaa ccttgggaac tatcatggat attcgacatc
    17401 aagctcaaag ccagaatatg ccaggtcagg atcacgaaca gcctgctgtt aaaaatggcg
    17461 caatttacgt taaagaaaat aaaggagctt aatcatgatt gtttttaacc ctttatccct
    17521 ctacaccacc tatctaggct ggcaacagta tgaggttcta ttcaacgccc tgtggcaaac
    17581 agggcttttg taccttggct ttttggccat tggttatcgc tttcttaaaa acgtgctcaa
    17641 tcccgcaggt gcgttctatg cggttgaaca cgctttaaat aattttcttt atgaactggc
    17701 agttactttc ctgatatgca gcttatttgt ctatccttgt gtaccgttgg aaaccaaagc
    17761 cctacagttt aaacccctgt gtggcctaaa aaacccaacg actgcggtca ttggcgacag
    17821 cggaacaact tatgatgaag cctttgcaga tctgctgact aaccaagtca aaattcccat
    17881 aggttttgcc atcattcaga actttatgtc gagctttact tacagtctca tgaaagtaac
    17941 gggctgcact gacagtttac agtccattca aggcgacttg gtatcaacct acctgccaca
    18001 gaatattcgc aaacaagctc tggattttca tagacaatgt tttatcgaag cgagaacgca
    18061 attcaacagt gagaagcaca aagccagtga attagatcct atgctaaaac gctatggcgg
    18121 tgaggatgat ctaaactgga tgggttctaa aatcctgcaa aaaatgtatt acagcaaact
    18181 tcatgcacgt aaacctgtgc ctggttttac tttccatcaa gcccctaatc gtaatcttga
    18241 aaaagcggct aatcgcggtg atataccacc agagcaattg ccagaagacg gctaccctag
    18301 ctgtcagcaa tggtggagca aaatcaaaac tgacctggtt gaagtctcta atcaagccag
    18361 tgtttttaac aaacatctaa attactacgc catgctggat agggttcgcc aatataaagt
    18421 gaaacacccg aaagcctgga aagcagacat cagcgcagaa gattttattg cgaaaatgct
    18481 cttgcaagaa agcagagaca tgcagaccag ttctgttcaa agcttcatgg ataataacaa
    18541 tggaaaggtc gcatctgcca ttacccatag cttggtcaat gtcggacaat ggacaaaatc
    18601 atggacatct accccattaa aaagagaagc catcatgcaa accttacctg taatgcaggc
    18661 gtttttcatg ttctttttaa tcattctcac cccaatggtt ttgtctttga gctgctacag
    18721 cacccgagca ttaggcagcc tgtgcgcctt atttgtcatg gctatttttc ttcaatacct
    18781 ctggcattta gtcggatttc ttgaacgttc agtgcttgac ccattaggtg aaaacgatgc
    18841 gatttctgct atgaagaata tggctgtgat gttttacttt gtcgcgcctg ttttattgct
    18901 gagattatca agtcattttg gcggtgacgc tggggctggt cttatggatt tggttaacgg
    18961 ttcagatagg cagagtgaaa gcatgacgca atccggtatg gacgtagcca agacaggagt
    19021 aaaaattatt tcaaaaggaa ttcgatgaat atgttaaagc acgtccttac gttcaaaggc
    19081 ttcattgtat tcacgaatag tgatcaaacc attatcaagt ttatcttgag cctcataaac
    19141 agacatatag cgtttggcag gtttatcctc tgccactccg acaatggcat caagaacgac
    19201 cgccaacaac cgaaatatct ttctcatttg gagttatccc tatgattaaa cattacatcc
    19261 gttcatccat tatagtatta attcaaacca ttttaccaat gattgcctta ctagtaattg
    19321 cgccttcgtt cattaatagc aatctactag cacactggca aagcacggtc actaccattc
    19381 aaccattatt tcttggtctg catggagcgc tctatttagc ccttattcta ttatggccaa
    19441 gactaatttc acgcttacag aatcaacacc aacttaccac agaacagctg agcatcgcct
    19501 taaaagctcg ttggtatctg ctggctattt ttgtatttat tgatgccttg atgatcggga
    19561 gccgactatg agcaattacc ctattaataa cctattaaga gaaccaacag aagcctggtc
    19621 agccattacc tgtttgtctc tagccttact ggcatatact caaccgcatt tgtttttact
    19681 aacacaaact atgggattat atgctgcgct cgcattatta atccctggaa cttatagagc
    19741 ctggcaagca ataaaagtaa aacgctatca ccgaagatta ttagccatgc ccacctatgc
    19801 cctatccacc acacaaatac ctttatccaa aaactggttg ttcctgggca aaggctttcg
    19861 ctggcgacca agccataccc aaaaactgca tcaaattaaa caagtcaaaa atgaaaagtt
    19921 catgcaacga agctgttgct accagcttgc cagaagattc tgccaaaacc atgaacatac
    19981 aaagctagcc aaatggctaa ataccaattc aaaattaaac ccttttagac ctgatccacc
    20041 tgttggcggt agtccatttt tgcatggtgt aggcgaagaa gataaacctg tatacatccc
    20101 tcaagatgta cgagtaggcc acactttcgt tgttggtaca acccgagtgg ggaaaacccg
    20161 tttagccagc attttgatta accaagacat ccgcaacggt gatgctgtca ttgttgtaga
    20221 ccccaaaggt gatcaagatc tggttagaga tatgatcgca gcctgtaaag tatcaggacg
    20281 aatagaagat ttcaaaatag tacatttagg ctttccagaa caatctgcac aatacaaccc
    20341 tttaaagaac ttcgaccaaa tcagcgaagt tgccacacgc atcaccgatg caatctccgc
    20401 agagggagag ggaaaacagt tcgcagcctt cgcatggaaa tacgttaata ttgttgctat
    20461 atgcctggaa gaaatgaatc aacccattac ctatcaaaca atagccttct atattagtcg
    20521 attagaacaa ctgttaatgt cctatgctga taaaattctg cctaaccact atcctaatta
    20581 tcatcaagag atagatgaaa tcatcgagga acacgatagt aaaataggaa aaaataataa
    20641 gacaaaatcc ccaatggaac gccaccaggc ggtcattcag tatgtcaaag accacatcaa
    20701 caaaaccatc aaaagcaaca atgctgaagc cctacacgac caaatcttaa tagacctcta
    20761 cgatgcagcc attatggata aacattatta cgacaaaatc acggccagtg ttggtcctgt
    20821 actgtctgaa atcaataaga gtaatgcctc aaaaatcctg tcgtcaaaag ctcatccagg
    20881 tgaaattgag ctgatggata taattaaaaa taagaaagtg ctttatattg gtttagacag
    20941 ccttactaat cccaatattg ctcaagctgt aggtaaagcc atcttgtctg atctggtatc
    21001 tactgctggt aaaatctata aagaacccaa cgcacgatat actttaaatc tgcactgcga
    21061 tgaattgtca gagattatcc aagattcctt tgtgaagata cttaataaag caggtggggc
    21121 tgggtttcag gtcaccgctt atgctcaaac tatccaggat atggaggttg cacttggctc
    21181 caaggctaaa gcagaagtat ccgaaggaaa ttttaataca ttgattatgc tgcgtgttaa
    21241 aaatgaagaa acagccaatt tactggttaa agtcctacct caagttggtg ttgttggaca
    21301 tacgcaggtt tctatggtta acgatacgcc gcatggggat gatgggattt attttaatac
    21361 gactaatgaa gatcgagtcc agacttcttc tgtgcctatg attggggtgg acgatatagt
    21421 ttcattacct aaaggtcaag catttattct aactaacgga ggagatttat ttaaagttag
    21481 actaccttta atgaaatcaa tttgataact tatagattga tatcaagaga ctaacgtgac
    21541 cgactttcta aaaaccaatc gcttaaccat tagttttact gaacgaagcc attttgaaga
    21601 tattctccaa ttgcgctcag atcctgacgt acagcgattt actatacaaa gacctcaatc
    21661 acacgtaggg atattgaacg atttatggat actatgattt acaaccagaa atagaaattg
    21721 gttatcgttt tcataagaaa cactggggta aggggtatga gacagaggta acaaaagcca
    21781 cttgttgatt ggaggtttaa aaatctgaat atagatacta tcgtctcctt tcaagaaatt
    21841 tgccagatga tgggtatttc aaaaccaaca ctgtatgaat accttaaaga aagagaagaa
    21901 aaatagaatt atggccaatt tatgtatacg aagagcaaca cttgctgatg ccgaagcaat
    21961 cagtcatgtt atcattcaat cactcaaaaa agtaaacgcg aaggattact cggagagtgt
    22021 gattaattca gtgattaaaa atttctctac agagcaaata gctacgaaaa tgaaactgag
    22081 aaaaattttt gtcataacag aaaaggacca aattattggc acagcaagtc ttgaaggaaa
    22141 catggtacgt acagtttttg tactacccag taagcaaggt aaaaaatcag gagcattgtt
    22201 aatgcactat ttggaagaag tggcacgtaa agaaaatatt tcctgcttga cagtatcctc
    22261 ttcaattact gcggagggat tttatcgaaa gattgggtat aacgctttgc gcgatgagta
    22321 ctatggggaa gaaagaacta ttatcatgga aaaaaaatta tccgagcttc cttagcgtac
    22381 attttcgtac cattggactt caaatcccaa aaacagacta aaacacgatt gccaatattc
    22441 agcacctact tcaatcgcac tatatgatac aatttattca aagcactata ttccaatcac
    22501 ttttggatga tgatttgata ttatggttta ccaaaagggt gaaataataa aatgaaattt
    22561 ccctatcacc tcatcgaatt aacccatact ctagatgaaa ccagcccttc ttggaaggca
    22621 aactgtggtt tttcgtcatc agtaatattg gattattgcc atgaattatc ctaaagaaaa
    22681 aatagaaccc aatcctgatg cgcaacaaat caatgaatgt attaatttag tcactaaagt
    22741 tttaggtgac aatcttttgg gcgtttatct ctatggttca gcccttgtgg gtggtttaca
    22801 aaaatacagt gatattgact tattcgttgt aaccaatcat gccacaacct tgagtgaaaa
    22861 gaatgaattg acgaaacacc tgcttcacat atcaggccgt tacatgaaaa gcgcaaaacg
    22921 gcctatagaa atgacggttg ttgaaaaagg tgcagttaac ccctggcatt atccccctcg
    22981 ttttgatttt caatatggag aatggttaag agattctttt gaaatgggtg agattgaacc
    23041 acagaattat gaaatgcccg atcttgcttt gattatcact caggttttat tgaagagtca
    23101 gaccttgtat gggaagaagt ctgaaaaaat tttagcgcca gttccctacg ttgattttat
    23161 taatgcgatg cttcaagatc tggatagact tgcagccgat atcagagatg atactcgaaa
    23221 tgtgctgcta accttggcgc gtatttggag taccttagaa acaaataaga ttcgatccaa
    23281 gcctgatgcg gctgattggg tgatagtgcg gttgccggaa actcacaaag cagtgatgaa
    23341 tagggctaaa gagatctgta tcggcaagaa ggaagaatgt tgggaagata ttaaagaatc
    23401 tttaaaacct tgtgccgatt tgatgctaca gaacattaac gaacttaaat catccgtcaa
    23461 tctactagat ccctgcaaac gcatccagct gatgcccccc acttttataa taaggctttt
    23521 aaagaaagag gatatagcac cttgtgctga gttatttttt aacactgttc atcatgttaa
    23581 taaaaaagac tacgaccaag cccaactgga tgcatgggcg cctgatgcca aactttttat
    23641 tgaacgcttt caggtgatgt taaaccggat tgcctgggta gtggaggata acggccaaat
    23701 ggttggtttt ggagatatga cccaagaagg ctatattgac cggttgtatg tccataagga
    23761 ttatcagcgc caaggggttg ctcgattaat tttttccaag tggcttgatt ttgcaaaagc
    23821 acaagacttg aagcaattaa caacagaggc cagcattaca gcaaaagcag cctgcgaagc
    23881 tattggattt actctgatgg aaaaacagat tgttgaaaca aatggggtga ggttcattaa
    23941 ttacaagatg gcttatcagg tgaacaaatg aaagagctta ctttttttaa gggttaccaa
    24001 caagaggata aaaaacgcgc cgcgtttaat cgccttgcag ttaatacctt tgatttatct
    24061 tttgaagaat ggtaccaatc cggctattgg cgagataaat acatccccta taccttgttt
    24121 gatggagagc acgccattgc caatgtttca gtgaatatca tggattttag catctttggt
    24181 catagccaaa gagccatcca gattggcact gtgatgacag atgaagctta cagaaatcaa
    24241 ggcttaagtc gaagactcat ggaaaaggtc tttgaagatt ggaaagcaga tagccatttg
    24301 atttatcttt ttgctaattc aagcgtatgg agcttttatc ctaaacttgg atttaagtca
    24361 gttaaagaat atcaatacca gcgcacaatc acatccagaa tacatgctaa ctttgttaaa
    24421 ttggatatgg ataataaaga aaatagagaa aagcttcata attatgcgca aaacacctgt
    24481 ccatttggca agatttccat gcaagaaaat gccgatttga tcatgtttta cggcatcacg
    24541 gtatataaaa ataatgtctt ctatctccct gaattggata ccatcgccat tgccgaaata
    24601 aatggacgac aactccattt attagacgta tttagcaaaa aagagcaaaa tttaaacgat
    24661 gttatccatg ctttgagtga tgaaaccata gatactgtgc gacttggttt tgtccccacg
    24721 gattgttcat cctatgagat aattcctatc gatgaaaaag aaaaggatga aatgcttttt
    24781 gttgagacag aaaaaactgc tttattcgat gaaaatcagt tgatgtttcc cctgttgtcg
    24841 catgcataga ttaagagggt ttaaatatga atgaacataa cactttgatt ctgttaaatg
    24901 aactatcaga aaaaatcaat aacctatacg gctttgtcaa aattgctggt gaaaattttg
    24961 gagaaccggc aataaactcc ggtccatgtg gcccttttgc taatgctttt tatactattt
    25021 ggaatcaaaa gtttactgaa agagtaaaca ttgcctttat catggtaaaa aattctgatg
    25081 agtgctggca tgtattaatt agattgccaa atgggttact ttttgacggt ggtattggcg
    25141 ttcatccaga tgatagatgg gataaaaaca aatttgatat agtggatatg ttccaatatg
    25201 acctgcaact attagaaaaa cattccggtg gtttgaatag aaaatatcct cgatattgcc
    25261 ccaatttttc tattaatgaa gtcacccatt tgataaccaa ttgcctcaat ttgattgaag
    25321 aataaatgaa tatgcaactg ttaatagata agctgcaact tactgaacta acagcacagc
    25381 cagaattact caaaagcggc ttcttttaaa ctaagtcaag ctttattgtc agttgcaaaa
    25441 cactaatata attgatcacc ataatctgca accaattttt tatttgaaga acaacctaat
    25501 tgttttcgaa ttttatcaaa ataatactcc accgtgcgat aagaaatcag aagttcgctt
    25561 gcaatttctt ttgctgattt tccttgcacc agcaaaagca aacaacgact ttgttgcttg
    25621 gaaagatgaa taggtaggtg cagctcctta tgtaacagcg ttagtgttgc ttctgagttg
    25681 tttatctggc atttcttcaa atttactacg tcactggtgg taggcaatgt taatctatgc
    25741 tcaagcgatt tcgcttctaa aataagatca cttgcacact tattgtaccc cacaatgaag
    25801 tcgtttaaaa aagcgccatt attgaccacc cattgtaaaa aatcattctc acttaaatca
    25861 aaaaataatg agtaaaagtg ctgacaatcg tgctctttgg taagaatatg aaccgagttt
    25921 tggccatagt tctgcttata ctttaaacct attttcctca aaagaggcat taacaacgca
    25981 caatctttgc gactattttc aagggtttta tttaaataga cgccctcagc taaggttctt
    26041 cctgtcttgt ccgtgcataa tgctggaatt tgatttcgct tataatagct gacaatattc
    26101 ggctcactaa tgatcattga tatttggtta tttccaaaag taatatcgtg ggtgaaattt
    26161 acgagagcaa tattttgatg tgatgcgcaa tttgattgaa tcaattcact tgctaacgac
    26221 agcatataaa tcatcactat tctttagaat taactagaaa tgtactacaa ttaaccaatt
    26281 atttcaaatc aatttaccgt atttatacgt atatcatttc agatccaaat caactaaaat
    26341 ttactataat gtacaaataa agacctgctt gaatggattt cttaaataaa cttaagatta
    26401 gggagcgtaa tatgtccccc aaaaattcaa atactgacaa aaatgaagac cctcgaaaac
    26461 aagcgataga aagcaatcct tgtggtatat gccgttcagc tggcattcca aactgtaagg
    26521 gtcataaagg aggtgctggt ggcggagaaa cttcagatga agaatcggaa gaactgattg
    26581 atgaacattt gtcctcacca atgacggctt ttaaacccag cgtaaatttc ataaaaagtg
    26641 aagtgtggag acaacctgat agttgggagt ctgtttttga attcaagaat cctgacgcat
    26701 taattacgat caaactggat ttcgaacaag gcatcctatg tttatcaagc aaagaacgac
    26761 taccaaaaga tcaacaaaaa gcagttgacg agctttttaa cgcgataaaa aatgaattta
    26821 accaatttaa aaaagagtta gccaatcagg gaatctccgt tgaaaatatg caattaaatc
    26881 atcagggaaa caacttaacc cttaatatca ataaaccaaa actctttgat gcatttatcc
    26941 aaaggttgat ggataaaaat ttgttaatag ctcaagcgcc aacactgcaa aatgataaac
    27001 aagagaaatt ctctgctacg cctgaaaaat caatgaatac aacattggac gctaaacaag
    27061 attataactc aaccgcacca acaccatttg atatgacacc aaaacccaaa tactaggata
    27121 tggatgactt aaactatgaa agaagctgca attgaattcc taatttataa acccaatcca
    27181 gaacatcctg tgccggaaga gcaaagaggc gtggtggtaa accctcacct aaaacaacgg
    27241 tttaagcagc aatttcttcc aattggtgtc tatttagaag gctccgattt gaggtattat
    27301 tttttcgacc aatgtgatga agccccagaa atctatagta gtttggatgc cctcaaatta
    27361 gacttacccc aggaattaaa cactatattc gaaaaaaaac cgaaattata tattatgggg
    27421 catgctagtg gtggccatta tggcatgggg aattgccatg gaccaagtga gcagttattt
    27481 ggggatcatt tcgatcattt attaaacagc ttaaaacaaa ccttgcccgc agatcatggt
    27541 gaggtttttg tgactttaga aggttgtaat acagacaatc aactgaatgc tacagaaaat
    27601 caacaagaaa aaacgttttt agaaagagta tcagcgaaac atagagaaat tacgtttggg
    27661 gggagcagac catggaacgc tcaagaggca ctaactggcg cccgctcgct cactccagca
    27721 gcgcctatca catcaatgac aggaaatgtt tggaagtctg gaaacagtgt tatttttcat
    27781 catggtcaat atcaaatcgc tgttcgtaaa tctttatttg cttcaacaca aacggctaaa
    27841 gaactaaaaa taaacaccat agaatatgca agtgcgatac taggcaataa cagccgtaat
    27901 gaattaattg ccaagatagc attaaaccga gatattctaa ctattcagga tttggaaaaa
    27961 atcgatggtt ttccaaaatt acccattgaa gaagagaatg tttccagctt tattgcgcaa
    28021 gaaaagctaa ttttaaacaa agagcaagaa cactatttag cacgtgttcg tggcatcctc
    28081 gagcgagcag aaccgataga tcaactgacg gatagagata tactggatct tctccttggc
    28141 ttaaaagaac cttctgtatt tatggatcat gaatattttc tagaaagtat tttggccaat
    28201 aaagctttgt tgcagttggc aatagttagt tgtggaaaag tccttgttgg tggtccaagt
    28261 aatgatagtc tcattgattt attattaaaa catggtgctg atatcaatag tgccgataaa
    28321 gaagggatga ctgcgttgca ctatgcagta cagaactttt ataattacag agaagagcca
    28381 ctgaatctca ttaaaaagct aatagactgt ggcgccagtc tcgaagtgca aaacaagaaa
    28441 gagcagactc ccatcgacat agcgcgggaa catagtcaag atggaagggt tactgctagt
    28501 gataaacttt tatcatcttt gaaacatgac cattccccac ctactcccct tgaagtaaaa
    28561 caacttgtta atcgaatatt tcaaatgagt caacacaagt cagatgaact actttcatta
    28621 cctattgcga cacgtgaata tttcaaatgt agagatggca ctctttatat tccagaagat
    28681 gtagtggaaa aattacatgg ggtccttcaa tctgcaaaag gcaatgatga actcttgtca
    28741 gcgtttgaat cagagcttag tgcagttata agcgctcttg aaagaggtat tgggactgga
    28801 gaggtgtttt ctgaacaaga tgaatttatc cctaaatgtt tcatggatgt aaatctaagg
    28861 ctgacatcag ttatggacac actgattgag ctgaaacaag atatggctgc ttcaaaagtt
    28921 actgagcctg aaattgtctt tgcggatcat cgccctgagt cttgttaaaa gtcaaaaaat
    28981 ttatatttca ggcaaattaa tccagataaa gtttcattcc ttcatggcta gcctcaaaac
    29041 ccaatttctc ataaaatttt ttagctcttg cacgtttttt gttagttgtt aattgaataa
    29101 ttgaagcacc ttgggattta ccgtaattga ttgcggtatt tatcatccat tcaccaattt
    29161 tctgacctct gaatttttca aaaaccctta ccgcttcgat ctgcattcga gtttgaccga
    29221 taaaggttag tgatggcatg atggttaaat ggcacgttcc cataatttca acatcattct
    29281 ctacaatcat caggtattga ttcgggtcac attcaatctt gtagaatgca tcaatgtaac
    29341 gttgatccat ttctggctga atgtattcac gagctctacc aagttcatca tcctgaagca
    29401 gaaacacgat tgagggcaga tcatttatat tagctttgcg gtgcctgata atttggttca
    29461 taattcattc ctgtaggcaa ataaacaaat gaatagatat gagtaaaatt ataaaagttc
    29521 caaatggtaa ttaccatttt attacaggtt atcagaagaa tgatctgtac cgtcatgcat
    29581 tcaacgatct ggcagtaaga gggttacgat gagcataaaa aaatcattga taatgattac
    29641 gggagctagt tcaggaattg gcggcgggat tgcggacatt ttttcagaaa atggctatcc
    29701 tcttttatta atttctagga atattacttc acaacagcat catcaattac ccaataccct
    29761 atatttccag gcagatgtta ccgatcccat ttctcttagc cacgctatca aggaagctga
    29821 aataaaattt ggaccggttg aatgcttaat taataatgct ggtactgtaa aaaacggtga
    29881 attctctaaa ttatcacacc atgatcacga gcaaatggtc gatgtaaatg taaagggcgt
    29941 tatcaattgt atagaagccg tactacctgg aatgcagtca agaaaaacag gaacaattat
    30001 caatatcagt tcattggcag acagaaaaac gcgtccaaaa ttagccacct atgcagcaac
    30061 caaggctgct ataaagagtt taagtgaatc tctaagaatg gccaatgctt cattaggaat
    30121 tcgcgtatgc aatattgcac ccgccaaggt aaaaacctca ctccttcagc aatcaaacct
    30181 gcaagaaaac caaattattt cagttgctga attggcaaaa gccgttttat ggatttatga
    30241 acaaccccaa catatctgca tccgagatat tgtgattgca ccgacttact atgaagatta
    30301 gcagtgaaca tcaattgctg ctcaaacgtt tgctgctggc acgtataaaa atagacaatc
    30361 agtctctgac aattcattgg atttatcgga caatccagtg tatcaataaa cgttaataaa
    30421 acgccttccc ctctaagagt ttttctaatc gaataagctc taagagaggg attactctgc
    30481 tcttccaatt gtagcaaaaa agtactgtat ttttacggta ttaacttaac catgcgattt
    30541 tgataaaatt atatccaatg atttttggac gaaacactcc cgaaccttac ccacaaacat
    30601 aggcagttct gatgtaccta tttattttat tgctaggact tacaattgtg attgcgcgag
    30661 tgattctacg cacggctcat atttcaaatt cacgagcaga agctgaagaa tccattgtat
    30721 tacagattga tgaggcaccc gattttgtgg atgaacccga ggaaaaagaa atatccgcac
    30781 tgcagattat ctcaagcctg ggtaccttag ttatttcgcc agaaaatcgt ttacgcattg
    30841 cctttgctac attacttacg gctgcaaata ccggattaaa ttttataacg ccatggttgt
    30901 ttgctgaaac actgaattta ttatcaggag aggatgaaac aactgaaatt ggaggtgtgg
    30961 aaatttcctc caatatgtta attacactac tggtttcagc atatgcactg tcgcaagtga
    31021 ttcccaattt gcgtgatcaa accatggtac cggtcaccgc ttacaacacc aaaaaaatcc
    31081 tgatgatggg aactcaacat ttgctaaaaa aatcacttga ttatcacgta aatacccctt
    31141 tttcagacca aatttatcta atacaaaaaa gtttttcgtt gagtaacatc ggcaccccac
    31201 tgcttaccca aatagcgcca acagttgtcg aaattgcgat cgcaagtagt gttttagcaa
    31261 gtcgatatgg ttatggggtg ggagctggtt tattcgctgt tatggctgca tacacaggct
    31321 atagcgccct caccgccaaa cctattattg acgcaagaga aatgatgcta aaaacaggaa
    31381 gtgaagctta tcaaagtttc agtgatgcca tagcgcaata taaagtcatg cgcgattttg
    31441 gtcaatacga gcatacaatg aaaggcgtcg atgcggcatt aacaaaaatg atgcatgttg
    31501 aaattgatgc gcatattaaa ccccaactaa tctcattagg gcatatcagt atttcgcgcc
    31561 ttggcatgct tttggccacc ttgtatatgg gaaatggcgt taaatccggc gcttattcta
    31621 ttcaggattt tgtcatttta acaggctatt taaattcact ctccgcttta ttgcctgctt
    31681 ttggtcaagc tgtaaacgac ttatttgcat catggcccga tttgaaattt gtctttaaag
    31741 agctggctat acccgatgag gtagttgatt taaatccaga aacaccactt ccaattgaac
    31801 cagaatcacc tcctgtcata gagtttgata atgtaacttt cagctataca gctaaaccag
    31861 gacaagcacc cgtggtctta tttaaaaacc tttcattcaa gattgaatcc ggtgaaaaag
    31921 tgatatttgt gagtgaaagc ggtgctggca aaacaactct tttcaatatg ctttatggtt
    31981 actatcaacc taccgaaggc accataaaaa ttaacggaaa aaatattgct gaagttagtc
    32041 ttcaatcact ccagaaaaat atcactctgc ttgggcaatc accgaattta tttaatggct
    32101 caatccggga gaatatctgc tacggttcag aaaaccctga tgaagtcagc gatgaaacca
    32161 tttggacatt ggctgaggcg ttaaatttgc gcacgtttct tgaatcgttt gaaaataaat
    32221 tagatactaa cgtaggtgaa aatggaaaga cactgtctgg cggccaacaa caaaaagtag
    32281 ccattttacg aggattcatg aaaaaaacgt ccattcggct tatggatgaa attactgctc
    32341 cttttgatag tcaatcagca aagtcggtat tggaaagtat tgataaacta tcgacaagcg
    32401 ttacaacaat gatgattact cacaagctta cagagggtca aaacgcttca aaaatcatcg
    32461 tcctggcaga gggacgtgtc attgatgaag gctcacatgc aactttgctg caacgctgta
    32521 acctatatca acatttatgg aacgcctata ttgctcaaaa tgaaacgctt tcttcatctt
    32581 ctaccgctcg tatgcttgca tcccttggtg ggccgcaaac ttcggaaaca atgatattgg
    32641 aacataatga tccatcaatg gcattcgtcc aaaatgaaga aacaaatgat ttcaaacatt
    32701 gcgctgatca aattaaaaca caatatactc ttcatgcgag ctcatcaagg gacacagaag
    32761 aaaggcattt cgattcctct ccattagaaa taacgccgaa accacagtaa gcgaaccttg
    32821 gtcaatcctt caaaaataat aataatttag actctgaaca gcttgaattt agcgacttcg
    32881 taaaagatga aacaaatgtc ctatatactg cacttgagtc gtagcgaaaa tttccagaat
    32941 aatatgagct gcttactgtt gttaatcctc aaattattca gtacctgctc tttctttgtc
    33001 aggtaaaaat cctccggctt gcatctgcca cattctggca taatgccctt ttaaaaccag
    33061 taaagaatca tgcgtaccgt cttcaatgat ccggccttta tcaaacacca aaatccggtc
    33121 cattttcgct aaagttgaca ggcgatgtgc tatcactatc gtggttttat tttgcattaa
    33181 atgatacaac gactcctgta tgtattgctc agtcactgag tctagtgcgg aagttgcttc
    33241 atcaagaatg aggataggcg cattttttaa tatggctcgt gctatagcaa ttcgttgtcg
    33301 ctgaccaccg gatagcttga tccctcgctc acctaccatg gattgataag cttcaggtaa
    33361 ttcgctgatg aactcatgac aatgtgcttt tttagccgca tcaatcactg cttcatcgct
    33421 agcagtcgga tcaccaaatc gaatattctc caacaggttt ctatgaaaca atgtaatatc
    33481 ttgtggaatc aaggcaatat gttcgcgcaa agactgttga gtcacatgat tgatattttg
    33541 ctcatcaatc aaaatatcac cagactctac ttcaaataaa cgcaaaatta aattcacaaa
    33601 actgctttta ccgctgcctg aaaaaccaac taaccctact ttttggccac catctataat
    33661 aacactctta ttatcaaata aacgagcgcc ttcatcatag tgaaaagtga cgttatcaaa
    33721 ccgaatttca cctcgattta atttaagcgg taaagcatcg gcggcatcaa cgatttcatg
    33781 cggttcgacg ataatactta gtgcttgctt acaacgccct atttgttcag taaaggaaac
    33841 aaactggccg gctacaaacc ataagttcca caatatactt atagataaag acgtgacaaa
    33901 ggtaaaatct ccaatggtca ctttatttat actatacatc ttcagtaaaa cgataaggtt
    33961 taaacccagc aaacaaataa tactgatgtc ccaaagcaca cgcataccga taatttttgc
    34021 ttgcaaagcc tggtctcttt ttactgcttc tttcacagag ttttggatgt actgattttc
    34081 cgttgtaaag cggtcgaaca accgaacatt cattacatta ctaacgctat ccaccatatt
    34141 gcccacaaac tgcgtgatac tttctgcata gtgttgcgat gcatttttaa tcgggcgtaa
    34201 aaataaaaaa gtgataccta aaaagacgat tacccacgaa attaaaatca aagcaaatat
    34261 tggataaagg tataacaaca cccctatggc taccagaaag cctaaaaatt gggtatatac
    34321 atcatcaacg atattcagaa tattgatggt cgcgctctgc atatcattaa ttttattaat
    34381 caaactgcca gcaaaattat tttgaaaata acgatgcgaa tgctgattta accagcgaaa
    34441 catcgacatg caaatatctt ctcgcaaaac tggaaataat tttaatcgga tccaatccag
    34501 gaatcgcatg ctggtacaaa gcaaaaacca cagcgccaaa taacaaccaa tccaaggcat
    34561 gaccgattta aacaactgtt gtttatcacc tgaaaatgac acggcatgat caattatcat
    34621 tttcagagtc catggcatga acgtggttat aaacgcccag aaaaagccaa ttaaagtcat
    34681 caacaacacc aaccgtctgt aaggtttaag catggcgaag atataacgag ggagggttaa
    34741 tttttgtgta gtgttcatat attccttatc agcgacgaac aagctaattt aatgtcctat
    34801 aatccatcat attcgtgacc gtagagattt aacttatcgt aacataaatt gcttgcaaac
    34861 gttgagatta ctgacaccta atgactggca tggaaaaacc aagcaagtca taattgaaac
    34921 cattttgaga ataaattaac agcatcgtca ttttaggtaa tatttaaaca cagcacaaca
    34981 cagtagaatt acggtattga tactcccctt atcgtttact agaatgcgtg aaaattatcc
    35041 ttggcatctg tacctctaat cgataccatg tacatttttt ctgcaacatt tattcttctg
    35101 acattagttt ttgtaataaa ttagcatggt atcatgctat aattagttca tagtgaacaa
    35161 tttgcgatac tacattatgc caaaaattac aggaattgac cgcaaactga ttgaatccac
    35221 tatgcgtaaa gctcatatac caggggtcag tattgcgtat agagatggta aatcaaccat
    35281 tccgtcaaca acaaacattg gcacaaccga cacacgagat acttcaattc tgagcaaagt
    35341 agaggatgat actgttttcg gagcagcctc tttgagcaag cctgtttttg cttacctggt
    35401 attaaaactt atcgacaaag gtgttttaag tggaccagga aaaagtgctg gaagtggctt
    35461 agataggcct ttacatgaag tgttttcttt agaaaaattc tttcatgagc atgacaaaca
    35521 attaagtgaa atagatattg aacgagctaa agcaattact ccaagaatgc ttctatcaca
    35581 tacatccggt cttggcattg acgctagtgc gactttgagt tttgcaccgg gtactgaata
    35641 tgcctactcc ggtacggggc tcgaatatct acaaagagtt attaaggaac aaactggaaa
    35701 gtcactgtgt actttagctc aggagcatgt atttggtgag ttggctttgg atatgaaaat
    35761 gagtagcttt gttccaccgg gaacctccga agataaagca gatgccgcta atagcctcta
    35821 cacgacagcc agtgattata caaagttgat gattgcctgg atgcaggatc ccagtcctat
    35881 tatgcaacaa gcgtttgtaa gacaagtcag tttaacccaa gataatcaga gattgccagg
    35941 agaatcaaat cccgcagctg aacatgtaga catgaaagtt aaagagtgtc ttgcatgggg
    36001 tttaggatta ggcttagaac ttgatgagac tggcaaagca gttaaagcgt ttcatacagg
    36061 agatatgaat caatttcgtg cgcaaacggc actcgattta gaaaagaaat cctgcattgt
    36121 atattttgcg aatgctaata gcgatttgca ggcaaatggc catgttttag ccccacttat
    36181 cattaaaccc aaaattccca tcgattacgc ccatacatgg ttttactcaa aatttccatt
    36241 tgcattaaag cctgatcagc ttacggaggg ctctcgcttt ggattaaggg tacaaggaaa
    36301 ggataaaaaa gcagacagtg atgcggtcat gacagcattt atgccaggag gtgcgccaac
    36361 acatgttcta aaccctgatg catccgatgg cagcagctat gctgtgatga gagcgttgat
    36421 gcccacgcat tctccaacgc cagccttaat ttctcctgaa ttagaagaac aatccagtgt
    36481 ggaatcgcct cctacaacgg cgatcgaaga ggaaattcaa gcggaaactg atagaccttc
    36541 ctcaccatca ccatttaaat tgacacccaa accctctggt tattcttgat gttgtattta
    36601 cgaaatattt gtgtcacaga ccatttaaat acaagatggg aatcttctac tttatttagt
    36661 agatgttaag tgttctaact atagtaaaag atggctcaac aagacagacg attatcctta
    36721 agtaggttgg atattctatt gctccccaag ttcggatagg ggtatttttc atttatattt
    36781 tttcctttaa tatcagtatc gagagtaccc taataaatca gcaagcttga taaccagttc
    36841 cgtcttgttt ttacattgca gtttggcttt tatattaatc agataagtct ctactgtgcg
    36901 atgagataaa tttacacgtt ttgccatttc ctttgcagtt aaaccagtta tcaataattt
    36961 tgcgagggat aactcctgtt ttgaaagcga tgctctgtct ttggcaacca cagttttaaa
    37021 aacattagcg cttaatagac ctagctccgc aatacattgc agagattcgg ccaatgggtg
    37081 taaaccaagc attatggaac aaccgaagac ccctaccacg ttctgctgtt gataccaagg
    37141 cgattttatt gttaaatatt gataacttgt accttcatta tgctctaaca tatgttcgca
    37201 aatgttcaca gtctcttgag ccatcacagt cttatccacc gccacagcaa aatgtgcgct
    37261 ttccttattt aaaaaatccg aagcgatttt accaatggca gaaaattccg agtcaaaacc
    37321 acaattttga gcgcaggttg agtttatttt gccaagacta caatcatttc cttttacaaa
    37381 ataaatacta aagggttgtt ttagtatatt ctcaacactt aagtgcgcag tttcagtcat
    37441 agtttcaggg ggtaataata caatgccttt tttatatcga gaaaagacga attcattgct
    37501 aatattcata agcgtactat aaaaccaatt tatgtttatt atacacccaa aacaatattt
    37561 gcatatatat taattataat gcgctaaatt tcaacatttt tttgatcgaa atacgataac
    37621 gaaagattga aggaacatct aataagctgg cc
//
LOCUS       DAXLDT010000013        35401 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXLDT010000013 DAXLDT010000000
VERSION     DAXLDT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35401
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.013"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(263..668)
                     /gene="ssrA"
                     /locus_tag="ACF2SP_000545"
     tmRNA           complement(263..668)
                     /gene="ssrA"
                     /locus_tag="ACF2SP_000545"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(819..2834)
                     /locus_tag="ACF2SP_000546"
     CDS             complement(819..2834)
                     /locus_tag="ACF2SP_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFD2358352.1"
                     /translation="MSTTVGLPQFSHIKIEHFKSHLDALLKNHLEEIDRLLKENHHYT
                     WDNLIYPLDSLADELERFWSPFAHMHAVMDSEAIRESYETCLPLLSAYDAAIGHNQDL
                     YEAIKSIDQHSLNPAQKKIIEDSMQDFELSGVALSKANKKRFEAIQSRLAELSSKFEN
                     NVLDATHAYTIHITEAERVAGLPEHALNTAKELAHEKGLDGFVLTLEYPCFQAVITHA
                     EDRALREEMYRAYITRASDQGPNAGTFDNTPLIDEILSLRHEKAELLGFNNFAELSLA
                     TKMAASTNQVTEFIYDLISRTRDKGKAEFRQLEVFAQDKWNLSPVNPWDVAYLSEKRR
                     QDLYSLSQEELRPYFPQPKVMQGLFAIVKKLFGMSIEEIEGVDVWHKDVQCYCIVDES
                     NQTRGYIYTDLFARPHKRNGAWMDSMQSRRKLEDGTVQLPIATLTCNFAKPSANRPAM
                     LSHDEVVTLFHEFGHCLHHVLTQVDYLGGSGINGVEWDAVELPSQFFENWCWDEHALS
                     LLTSHVDTGETLPSALYERLIAAKNFQSAMAMLRQMEFALFDFRIHQEYQIGKASYVA
                     NILADVRSKTSVVPIVPYNRFQHSFSHIFGGGYAAGYYSYMWAEVLSSDAFARFEEEG
                     IFNPKTGHDFLKSILEAGGSRKAADAFAEFRGRPATIDALLRHNGIL"
     gene            complement(2934..4268)
                     /locus_tag="ACF2SP_000547"
     CDS             complement(2934..4268)
                     /locus_tag="ACF2SP_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358353.1"
                     /translation="MTPIISHLLKIFPELNTQVKTDSLNWTFSTHLKQLGPDFYSKIA
                     RLHPILNMEYSVLCQRLRYNLSAPDYSSPEQIKEQLIDALKLAELLEYTYQHYLVVPR
                     EVVRLRRHKAIYRELLTELSGYSFALDNPEPESLKTSLSLTQAIREKTAQSNWYRIFI
                     SRSKRVINLLDNLDTGSKAFRDFVVLLDKYTNPFLAYLGWCFFAPRLFTNLFLILKHT
                     IPGEWMGEKEKSLDWSDRFYAHLQRRWFELANDSVWFTVGILNCFVLVGALAPLSVYL
                     SLFAFVFDVANTSLRAYIEIGRLQQLQKEYAELFDQEENEDQKKIIKEYQDSISYRIK
                     FEILRSFLSVGGAVAVVIAMALSVPALGIINPVIPLIGAVLLLALWGVSLYLNNKLDE
                     YRPVDNIEKPAPSVINKLSFFAPKNEKKESLHLSSKPEKDLEAEELIPTFTF"
     gene            4454..6532
                     /gene="tkt"
                     /locus_tag="ACF2SP_000548"
     CDS             4454..6532
                     /gene="tkt"
                     /locus_tag="ACF2SP_000548"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFD2358354.1"
                     /translation="MNGLLADLCLLSDNSASVKCKRVGMNSFTELANAVRMLSIDAVN
                     QAQSGHPGMPLGMADIATVLWKKFLKFNPKNPHWFNRDRFVLSNGHGSMLLYSLLHLT
                     GYNLDINELKNFRQLHSKTPGHPEHGHTPGVETTTGPLGQGLANSVGMALAERVLASH
                     FNHDHYNLVDHYTYTFAGDGCLMEGISHEACSLAGTLGLGKLIVFYDDNGISIDGKVE
                     SWFTDDTTSRFKAYNWQVIGPIDGHDADQIEQAISAARENTTQPSLIICKTVIGLGSS
                     VAGSEKAHGSPLSAQDINNVREFFNWKHAPFEIPDSIYKQWDHREQGDKEEQQWLQLL
                     HEYRLQYPSEHDEFLRRANGDLPDDWQEISAGFLKQCLNNEKAIATRKASQQCIEFFA
                     PILPEMFGGSADLTGSNNTDWSGSKAITAHNFSGNYLYYGVREFAMAAIMNGLAVHGG
                     FIPYGGTFLVFADYARNAIRLSALMKQRVIYVLTHDSIGLGEDGPTHQPIEHIAMLRM
                     TPGMTVWRPADLMETAVAWQQSLEHHNGPSALLLSRQNLPALAHGTDAADLIKKGGYI
                     IADCEGKPDAILIATGSEVQLAITAAEKAKARGLQVRVVSMPCAERFLAQSDAYKNQV
                     LPGQVRTRIAIEAASSAYWHQFVGFDGAVIGLDCFGVSAPATDAFNYLGITVEKIINT
                     LDTLTKQTMV"
     gene            6613..7605
                     /gene="gap"
                     /locus_tag="ACF2SP_000549"
     CDS             6613..7605
                     /gene="gap"
                     /locus_tag="ACF2SP_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFD2358355.1"
                     /translation="MTIRVAINGYGRIGRCILRSIFEYNRQNEFEVVAINDLSGIDVT
                     AHLTRYDSTHGPFRSDVSIEGNMLIVDGHGIQVIAERDPAKLPWKQLDIDIVFECTGF
                     FTSRDAAMGHINAGAKKVLISAPAKGVDTTVVYGVNHSVIKASDVIVSNASCTTNCLA
                     PVVKVLNDAIGIESGLVNTVHAYTKDQMLLDGSHKDLRRARSATQSIIPTKTGAAAAV
                     GLVLPELAGKLDGFAMRVPVLNVSVVDLTFQAKRATSVEEVNDIMRKAKSRILQINED
                     PLVSCDFNHNPASAIFDTTETKVIGSLVKVVAWYDNEWGFSNRMIDTAHQMMNC"
     gene            7616..8806
                     /locus_tag="ACF2SP_000550"
     CDS             7616..8806
                     /locus_tag="ACF2SP_000550"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFD2358356.1"
                     /translation="MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTI
                     KSALDNGAAVIVLSHLGRPEEGKYEKKFSLEPVADYLRKNLEYPVRFVKDYLSGVDVK
                     PGELVVCENVRFNPGEKANDEALAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPV
                     AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIA
                     NTFLKAQGFEIGISLYEPDLLDEARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFN
                     KSLSNVAEDDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAI
                     AESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKH
                     VKTN"
     gene            8790..10214
                     /gene="pyk"
                     /locus_tag="ACF2SP_000551"
     CDS             8790..10214
                     /gene="pyk"
                     /locus_tag="ACF2SP_000551"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFD2358357.1"
                     /translation="MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSAL
                     QLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGD
                     INGVSVAYPNLASELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
                     KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAKITPII
                     AKIERMEALDHLTDIIREADAIMVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVIT
                     ATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLFPVKVITMVNKICLS
                     AEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHH
                     STVPIFAISANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGY
                     VLLTRGTQIGMPGGTNCMEIIPVV"
     gene            10306..15933
                     /locus_tag="ACF2SP_000552"
     CDS             10306..15933
                     /locus_tag="ACF2SP_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdhB"
                     /protein_id="HFD2358358.1"
                     /translation="MLLLSIEVIKMPDKKSSETLLSQQINEVRGLLLQALKTQLDDLV
                     AEKLIPQPSGVPFVDFEKDPPQIATLKKVINCLYHAEEAFKSWESIDASTLLGKAKAA
                     PKLIRALTQIYKSLGLLDEASPEIRGVIADNYHLLEPLFTQAYAIVQESGWLSEFMEM
                     DVTDKASKVIFQGMDLLGPDIDKWHDTHPLVDAFSKISKLVETISKLQEKDMPGKEKE
                     QAVELVHALLDDLDNNPFISKLSISDFEDSKAIKDLLEWFKNIQDDGFDFSKKSIKQY
                     VSWANHYLPTLIAFTDQLERQNYLKPGSLSANLCSQIEQLGKQVNDILSEPGFGITER
                     VVTIDSLQRQREQKIHNAQVKSVQVIMATEKQQAATADFYRILEAYKGMNLSQITEPD
                     RRVLRQLYPQIQTALAHASLDLENQLTSVLNTTGPEKTPTKPKSWWELAKGGLGYVAS
                     YVVNYNVDKTLGTRVSVDRFLSSQIASEQFKILVAEKAREKLRTGSDKIKETALQYQA
                     EARVNAIKSTLRKQPAQMEAPPSQFVAVKPSKLLNLRGTLTAIQEMQLSTTVNGTRTS
                     VDTLIHRHLPEKYQVCFSTLPYQVNKNDPELVKQIKTVENNLFQLEKALKDFEKIDLS
                     YGIMIRLHYFIAIASAASKLKSSVKELSPEGQKALAPLLQQIMTLGSSFSDTNYTAND
                     LSALQQLKMEGQLRPKSPSVASPDTIGTLPEETVKRTAKKPKEKTTELLDYADKISHA
                     RALLLDKFKTTLSPALADDLNPQKKGVPFVHLDQDPPQIAAIKKIINSMYHAECALKT
                     WHGIDTSTTLGKVAAAHQGVAALSQVYKSFTLFTEDFSDIHNLIRENHDLIAPVIIEA
                     NKLVNQYGWARQFSELDIAQQLGSILGQGINTVQPKTDEVTQTASLVKLFSEIPRLLN
                     NISSSLDQKSEKSIDELRISNKKIEAIGAVFELFFEEHAYLMSMFKGFSAISGLIELN
                     KKIQREGTNLQEATIRQYQAWLKEGYPNLMMTLDEIETRHYLTPGLLSAPIALELDQI
                     NDKLNEIIETKPDFKLQKIPLSFYMGDKRIERLMTKKTEHCLALLQIEEQKKAVNTFF
                     GILKHYSGQPLSNIKPNELAQLRLAFVEIQSAMANSNLELSNDFVAALNQLETSPPEK
                     TKKIKVTVSQLLKVQPAVENYLNEHKKSCELKIAVIDKAIEHVKDKSFELKSGASKEI
                     NISQLRRTFLETESKKPVVGPGELKRTSVSSLNTVRGNLAYIQELKMSSTVGMMREQF
                     SALTRDNFSTRVQKYLIKPQDKPLHMINDNDPLMVRQIKEVENGLYHLETALKQFEQM
                     KKSDSLVSQTKALLEIANHALQLKMAVERFSPELKEHYGPLVRTVFSFSNKIQSINYN
                     KEDWADLQFILNRAHKELLKRKTPRDHRIEKAFFEAGIREVQIDRNESVGKKTAKLGV
                     KYAHLASPQLEKGRAYLSSRYKSAFGQQPKVIRSFTREQLANEEFMDAEISRLKRTLE
                     EQYGFNIATIKVFLALLEQIQRAGAQAAEVAGMVNTLVTYDFPKIKENAYRDLLTKLS
                     QEEDYLNLKPGTLINPAMAAVNQLFLSAALELDMPFSKKLSILDDTTYVNILIRQTES
                     DLQTLKKKLSEDPSNHEIAFEIEIKNDKLAFLRQQMVLLEDKNPNQTRNVLLDMQFEV
                     SLRNHLVYTTLKAPIAQEYEQIAREYYKKNKNRFLYADDCSKELLQGLKEFEKKHLPD
                     HLIVFEAYDRLRLFSAKLPPKNQDVKEYIENINRELQNKDIPIEQRALKVKSLPNDSV
                     FIEKLSSADKGTHFLRKFMQFITIITASITEAIKTGGSLIYIYRQKQMEQSIKNIEKS
                     ISFKEELHTIRDPDSPSPRKGEGKEGEIEFTKL"
     gene            complement(16033..17103)
                     /locus_tag="ACF2SP_000553"
     CDS             complement(16033..17103)
                     /locus_tag="ACF2SP_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358359.1"
                     /translation="MRIATLFSAVLFISPLWAAPKFFPSQPALNGVYDIKGTELCSTA
                     KHTLAYLNKGKNYDPQVIHDGKVIKISLDRVKKTLEFICQNQKKLNNPDFVKQHFDFI
                     RWYPDLSQAKQLSANKPLLQNIPKDKILMTKYFVHLAGASTKPSSAKPYPLYALPEDE
                     QNLTLDEANTKPGLIRFRYGKQAILSGALKNQPVSELAYLSRDDLEAALLQGTVVADF
                     GPTAGKKIFNVHRSNNIAYDRTKTPYKQERFWYFKEVDGIKGYGKDAEYKITVNPKVT
                     FAADLAEFGLGKLLMIQYQDKSGRTMTKMGILADTGGAFNNNLYQIDYLAGSFAGKDA
                     LYRATRDLPDYVDAYFMILKQP"
     gene            complement(17104..17796)
                     /locus_tag="ACF2SP_000554"
     CDS             complement(17104..17796)
                     /locus_tag="ACF2SP_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HFD2358360.1"
                     /translation="MRKQALHPRTAVINKAQKNQSKRARSDALLWLAANFPEAFDNSL
                     RIRPLKIGIMSDILQHAEKAEQVGVSKSKLREAVVLFTRRLDYLACLKAREVRIDLHG
                     NPVAEVTEEEAENASMKIKKRVEKSVKNARKQVNAKAANHSYVNNQPSTVSSVKPMNS
                     FDSHPEPLLPIYPLRSSTYASQNVAMQSAKSPSVVVKHKAPKQYDPDAVARLKEKLGL
                     SRKAEDKKETTE"
     gene            18153..18338
                     /locus_tag="ACF2SP_000555"
     CDS             18153..18338
                     /locus_tag="ACF2SP_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358361.1"
                     /translation="MRIILSILAIFMPWLVLLIKDNPGGAVVALIMQATLIGWPFASI
                     WAWRAVNPLTPASQSKQ"
     gene            18518..19249
                     /gene="dapB"
                     /locus_tag="ACF2SP_000556"
     CDS             18518..19249
                     /gene="dapB"
                     /locus_tag="ACF2SP_000556"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFD2358362.1"
                     /translation="MRTRVIVNGANGKMGILACETLENHEQFEVVAKLSRQDNLGQSI
                     LDTKAQIVVDLTRADCVYENSLTIINHGARPVIGTSGLVETQINELTKLCEIKQIGGI
                     IAPNFSLGAILMMMLATKASEYFSEVEIIEGHHQQKLDAPSGTALKTAEMIAAARKKP
                     KNKLPLKELTPGARGGSHHDINIHSLRLPGLLARQEVLFGNIGETLSITHNSIDRRCF
                     MPGIVLACQKVLNLTNLVYGLEHLL"
     gene            complement(19353..21821)
                     /locus_tag="ACF2SP_000557"
     CDS             complement(19353..21821)
                     /locus_tag="ACF2SP_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFD2358363.1"
                     /translation="MGKGIILRVLENTILSPQVFDTLERLLPGYKVEYFKEQPDYRKS
                     IARRIDSLHDAFSFILKAYPLDPKHTSLTVATLSTYAAECKASCDLEKLTLEELHLEL
                     ERFTAKLVEAIAIAWKWPKGKAVKEAIASLNEAEQYVLMSRGRSDIATIMPIEMGSET
                     KYVLQYDESLSPVYEQWLTELKQLKEYNFPKTPAWFKNLPPYQQAYYCNLNLSSVDPK
                     KALQHFNTLFGNWGDIAKRSLNLTTELNQIHTNSPPYPSWFNELSPAQQAMIRVLSAT
                     PHEIKSSLKEFKKFMVEQARNDQYASTLSLVPKLPQWYWVLSEKQQYFLEYALKNAEK
                     VEDVVSYLSSRHRTLPAPANYGAHSLYLIDGEGKETLFYDKRYRSSHVASRDSLKFPE
                     DVQQRHVDSNLVKVMEFAKPQQPLLLQTLISPIHAVDYIPTVVTDFLPELPPDLDLYK
                     IAREAVTRSKRRHEIFQHNHPFNIAKRYYYTQATDTDSEFLLKTAQKYASSKPGLQAL
                     IDDYKAVLESPLGSATFWDYDGRELFLSSLEELIILNMGGYSYGSCVSGKDRKAVELL
                     HTDAMILYKAKYGNWPKFGIPKEKQERVNFINIVVDLYISRHQHELAGQNAPGSEGIK
                     TPDWYWPNDIAEAINERLGTEKALAYDDRLATDNEVKNISKDLRSFFLPENELHCLLI
                     AKQLGEKMCTMLYDVLSALINEERRFQKSSKDSWKLRWFSDKDVSSTPTGILNIREVM
                     HDENSGNDNVLRIGKIFAAVLNRPESDSSRTTATNSVYDRIRKLLQPLSSEATLQTLA
                     EEAILEWSSLFESSKRENSGLVYM"
     gene            21999..22175
                     /locus_tag="ACF2SP_000558"
     CDS             21999..22175
                     /locus_tag="ACF2SP_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HFD2358364.1"
                     /translation="MKSIEDFTGFSESGIDDAIQNALKKAGNPTHFEVVETLGSQDRQ
                     SDRRYQVTLKTLSD"
     gene            22286..23785
                     /locus_tag="ACF2SP_000559"
     CDS             22286..23785
                     /locus_tag="ACF2SP_000559"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFD2358365.1"
                     /translation="MGYNVPHYIGGQLINETDTEAQAIYNPALGEIIGQVHFASKATC
                     DRAVAIAKAAGNEWSQTPAIKRARVLFRFRELLEKYQMDLARIVTREHGKTLDDAKGS
                     VARGIEVVELHCGLVNELKGDFSAEVASHIDCHTFRQPLGVCAGVSPFNFPVMVPIWM
                     MIPAIACGNTFILKPSEQDPSAPVRLLELLSDAGLPPGVANCIQGNKTTVEHLLAHPD
                     VAAFTAVASTPVAEYIYTTAAAYGKRAHTFGGAKNHCVVMPDADLDQAANAIVGAAYG
                     SAGERCMALSVVVTVGQHTADALINKISPLIRAIRVDAGDVEGTDMGPLVSQAHRQRV
                     LAAIEKGVNEGARLIIDGRTFQHPQYPQGFYLGPCLFDEVTEEMSVYQNEIFGPVLVI
                     VRVNHFEEALALVNKHQYGNGTAIFTRDGFTAREYSQRVQAGLVGINIPIPVPIANHP
                     FGGWKRSVFGDTNMHGEESIHFYTRRKTVTSKWPITELKDSSFAMPTHD"
     gene            23809..24705
                     /gene="mmsB"
                     /locus_tag="ACF2SP_000560"
     CDS             23809..24705
                     /gene="mmsB"
                     /locus_tag="ACF2SP_000560"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HFD2358366.1"
                     /translation="MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQG
                     GAIAHNLQELAKDKDVIVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSS
                     RETHQIAKENHLLVVDAPVSGGVAGAAAATLTFMVGGEAEAYNAAHPILSAMGKKIIH
                     TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSK
                     YVPVPGILENVPANNDYKPGFAANMMLKDLLLSQNTAKSVHIETPLGSKTTEIYQQFV
                     DQGLGDIDFSAIIKHIGKTQEV"
     gene            24707..26590
                     /gene="acs"
                     /locus_tag="ACF2SP_000561"
     CDS             24707..26590
                     /gene="acs"
                     /locus_tag="ACF2SP_000561"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFD2358367.1"
                     /translation="MLPVHLRHPHLTPTEESLSEFWSEIAAQTIDWIQPWSITLQGGL
                     HKGDVKWFQGGLLNVSANCLDRHLPHKANQTAIIWEGDDENQNKTLTFAQLYSEVCKM
                     SNVLKSLNVRKGDTVGIYLPMIPEAAIAMLACARIGAIHTVVFAGFSAHALQQRLIAS
                     SCKCLITADAFQRGGKTIPLKKQADEASVDLNITKLVVKNLNTPTALNKNKEHWWHEL
                     KQTVSDQCMPEPMNAEDPLFILYTSGSTGQPKGVVHTTGGYLVQAAYTHQLIFACQDN
                     EVFWCTADVGWITGHSYVVYGPLCNGITTLMFEGIPTWPDAARNWRIIDKHQVNVFYT
                     APTAIRSLMRTGDQWLNSSSRSSLRLLGSVGEPINPEAWNWYHQKVGQGKCPIVDTWW
                     QTETGAIMISPRASDEVTKPGSARKPIPGIVPLLLNEQGHEINGAGEGLLAIKYPWPS
                     MARTIAGDHQRYCNTYLSNGYYITGDGAKRDEDGDYWITGRIDDVLNVSGHRLGTAEI
                     ESALVSHPKVAEAGVVGIPHDLKGQGIFAYVILKQGNKPDAKLQTELMERVKDEISAI
                     AKPDVIQFVNDLPKTRSGKIMRRILRKIACKEVSHIDELGDLTTLANPQIVEELMKNI
                     LKR"
     gene            complement(26687..29998)
                     /gene="cegC2"
                     /locus_tag="ACF2SP_000562"
     CDS             complement(26687..29998)
                     /gene="cegC2"
                     /locus_tag="ACF2SP_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC2"
                     /protein_id="HFD2358368.1"
                     /translation="MTPLFPTQGPITIRQGIGGSCYLLSSLDCILNLGADGEQLIKSL
                     FTQTEDGKVIVRIKRHEALKDNLQKNKMTGKYTHYVDELNNEDVFEISPERLKEIDNQ
                     YGGVKSNSLAIKILERLVSYYYAGDWSNTDPLASVVAHDIPDRIAGFTSTAFLGKFFG
                     IQAEDIPYSKLDDIIKLKLMNPDEPVYISMSYGKVDSFGKFHGRHALRIDKIIPKGSG
                     NYDFVLINPHDNSKTETYKLDDLNKRNCRFCLFNTSIHRASLTKKLLTLSNEDGRYVF
                     SNSGLQKRLISLEEMNLLTDNKIISSCISLHKQIPYLEKLFLKLSVEEKKTLTTCIAN
                     ADGSKKEFLKLFLTHIPTMDLLELVLREETSQELLGEVLTELALSSPVEENKLSPKAG
                     INFNSEAFLHLILKSAIQQKINQLAYMPEKAKQEIESGIINFYFGGASSSLTRASGLR
                     ALFIANVFSKKSIETLFPPKALFAKAIANYFTLKTLPDLLIEYLKSKDTSPIDEEFFD
                     VVLASATFKDPDEFFENLFRLSRINPEVAKALFVFSSQKINVLFGISLEEYAKKIALK
                     DSGEFKSWFESLSKPQPVIKIPEIDNVLRQQRVDDAKRVISDIVQRINSFPFSFEGFK
                     TVAHVNLNAEEFRGQLKKIVHSGELQNALQILDLPDGHPEVQKALERKLRMIDAAANR
                     RSDFLRKYETDIDEHVRQIKNFPIDFNDADTIVAIESQRILLNKKLHTLVKTEDLLGE
                     QFIANPKIKMVYYAQVEKINLRAELLQKRLLDEAQKVINSVEKRMDNFVIRFDDISSA
                     SAVEWQRNNLLQQLDNLVKPNQALLSAEKILDCNDLQPSIVRALQAKKQEINETADQL
                     IIKINAEEVVKSYEKQISEFPISFNRCQTVEEVIARKQDLIQSVRNLVGNKPDLLKAQ
                     EQLQLLSGEYHSDIKMALTDKVREINRQADVMSKRITDQIAATKETLNILAEIKFSDH
                     LKTIESMVKTLETKAVGDENYKRAAPIARTFYNNLLRAEERFKNSQLPKNVKCKDFHQ
                     DCVRAINAVIPVLEVHRGWKQVFADLASALATLCTLGGANLYAGRWRLFPVPTESEKI
                     VKDFSLSMQPLSVRA"
     gene            complement(30112..30714)
                     /gene="yihA"
                     /locus_tag="ACF2SP_000563"
     CDS             complement(30112..30714)
                     /gene="yihA"
                     /locus_tag="ACF2SP_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFD2358369.1"
                     /translation="MPINLYSKAVFLKSAARVNQLPEDSGYEVAFAGRSNAGKSSALN
                     CLTNNKNLARTSKTPGRTQLINLFSLDEQRRLVDLPGYGYAKVAMEVKLEWQKNLAHY
                     LEARQCLRGLILLMDVRHPLKDLDQILVNWALHRELPVHILLTKADKLSRSEVKNAVL
                     KVRQYYELAEHLVSVQAFSSVKKDGVEELISVLDRWYEWN"
     gene            30789..31391
                     /locus_tag="ACF2SP_000564"
     CDS             30789..31391
                     /locus_tag="ACF2SP_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFD2358370.1"
                     /translation="MKKLALVLILCSSSVIFAQENPQEAGQNKSTVCTACHGPQGIST
                     NPEWPNLAGQHEKYFVKQLKDIKEGKSRSAPTMTAIVANLNEQDIDDLAAYYAKMPVA
                     EGSTPEKYLKRGEQLYRGGDLNKHIAACIACHGPKGSGNAQAGFPLLSGQHAAYTIMQ
                     LQAFKDGKRTNDLNQIMQDISSKMSPEDMEAVAYYIQGLY"
     gene            31402..32016
                     /locus_tag="ACF2SP_000565"
     CDS             31402..32016
                     /locus_tag="ACF2SP_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFD2358371.1"
                     /translation="MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPL
                     ITEFFSYGCPWCYKIDAPLNDWATRMGKSAHLERIPVVFKPNWDLYAKAYYTAKTLAM
                     SDKMNAILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
                     LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA"
     gene            complement(32177..33475)
                     /locus_tag="ACF2SP_000566"
     CDS             complement(32177..33475)
                     /locus_tag="ACF2SP_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HFD2358372.1"
                     /translation="MPETIINIENLSKSFKKAPSQHLLVLEDVNFKLQEGEIVALLGK
                     SGSGKSTLLRIIAGLIAPSSGTVTYRGKPVTRPVEGIAMVFQSFALMPWLTVLENVEL
                     GLEAQGISREERRHRAIEAIDIIGLDGFESAFPKELSGGMRQRVGFARALVINPDVLL
                     MDEPFSALDVLTAENLKSDLLELWKEKKTNTNGILLVTHNIEEAATLADRIVIFGNDP
                     GYIRAELPVTLPQPRDPESPEYLALVDKIYTLMTTGPKEKAKRAQRERQIGLGYRLPD
                     VEPSELSGLIETMKSFEERIDLPELADELMMNIDDLFPILETLEILGFAKVSAGDIQL
                     SELGKQFSEADLQERKQLFAQRLLEKVPLARYIRRVLDEKAGHRVSEERFLSKLEDYL
                     SEKEADRVLKTMIDWGRYAEIFAYDFNTGILSLENPGKGA"
     gene            complement(33483..35222)
                     /locus_tag="ACF2SP_000567"
     CDS             complement(33483..35222)
                     /locus_tag="ACF2SP_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2358373.1"
                     /translation="MGNSRFYFANPDKFSRFINRWDLLLLILTFSVLFFLGWAGSQMA
                     TPYQLGDQIPISLEPSNLPFYALRTVLRMFIALIFSILFTFIVGALAAKNRRAEQIVI
                     PAIDILQSIPVLSFLSITVTGFIHLFPNSLLGPECASIFAIFSAQVWNMTFGFYQSLK
                     TVPHDLIEVSAMFRLSAWQRFWKVEVPFSMSGLLWNMMVSMSASWFFVVLSEAISVAH
                     QNIRLPGVGSYIALAIAQRDLHAVGYAILTMVIVIFLYDQILFRPLIAWSEKFKMEQS
                     PDESEYQSWLIDLIRSSRLMKRVTEWLAVFTNSFVNARWLRIGEVKAVKEIDFKRQKR
                     LDRLWTALVLIAVITGGWFLLKFILAELKVSDIFHVFLLGAATGARVIILILLSSMLW
                     IPVGVWIGLRPRLAQKIQPIIQFVAAFPANLFYPLFVIAIVKFNLSVEIWVTPLMILG
                     TQWYILFNVIAGASSIPRDLYLAADNFGLKGWIWWKRLALPGIFPFYITGAITAAGGA
                     WNASIVAEYVSWGNITLKATGLGEYIQASTTAGDFPQIALGTAMMCVYVLAFNHLIWR
                     PLYRLAEERFNFN"
BASE COUNT        10868 a         7365 c         7064 g        10104 t
ORIGIN      
        1 atcattcatc acaggtaatt cattacctga ccataattgt cattcatttt gtaatgtcct
       61 ttagtgtttt tttaaattat cttgccctag ctttttgggc agatattggg tactttttca
      121 aaaattttgg gcatattttg ggtgattttt gagccaacag aaaaccacaa aagacaaccc
      181 tatcaaccag aaaagtggat ttaactcgtt gttctacaag ggtttatgat ggtctttggt
      241 ggtcaggaat ggttttgata attggtggag gcggcgggaa tcgaacccgc gtccgcaagt
      301 cctctgcctt tagctctaca tgcatagcct tgtcttttga atttaactgt ggctcctccg
      361 acgggcagga tgatacacag cgattccctg agtttcgcgc cataacccgg gatagattag
      421 gacgctagct tatctcttat gaccgttgat tcgataccga taagcgggct cggtcgaacg
      481 ggatactggg tttattggcc agtacacagt gatggtttaa ctaaagtgct tattaggcag
      541 cgatagcttc cccaccagca aagttttcat cgtttgcatt tatatttaat tgagtcttat
      601 ttacgagaga tctccttctc ggcatgcact tccggttttg caacccacgt cgaagccagg
      661 tcgcccccca ttctgtacac tttaattata acataaaatg ccggcgtgta agcaaaatcc
      721 cgccagcata ccgaatatat tttcaggatc tacaaaaaat cccaaggtca aggcaaaaaa
      781 tgttttaccg agaggattgg ggggcacaag tcctgttttt ataaaatccc gttatggcgc
      841 agcaaagcat caatcgtcgc gggtctccct cggaattcgg cgaaagcatc ggctgctttt
      901 cttgagccgc ctgcctccaa aatggatttc aaaaagtcat gtccagtttt ggggttgaaa
      961 ataccttcct cctcaaagcg cgcaaaggca tcactggata gtacttctgc ccacatgtaa
     1021 ctgtaataac cagcagcgta accaccccca aaaatatgcg agaaactatg ttgaaagcgg
     1081 ttataaggaa caatgggtac aacactggtt ttagaacgta catccgccag aatatttgcg
     1141 acataggacg ctttaccgat ttgatattcc tggtgaatgc gaaaatcaaa caaggcaaat
     1201 tccatttgtc ttaacatggc catggcggat tgaaaatttt tagcggcaat caagcgctcg
     1261 tacaatgcgg aaggcaaggt ttcccctgta tccacgtgcg aagtcagtaa agacaatgcg
     1321 tgctcatccc agcaccagtt ttcgaaaaat tggctgggta attccaccgc atcccattcc
     1381 acgccattaa tccctgagcc gccaaggtaa tcgacttgag tcaaaacgtg atgcagacaa
     1441 tgaccaaact cgtgaaataa agtcaccact tcatcgtgcg ataacatggc cggtctgttt
     1501 gcagaaggct tggcaaaatt gcaagtcaag gtcgcaatgg gtaattgaac tgtgccatcc
     1561 tccagttttc tgcggctttg catggagtcc atccaggcac cattgcgctt gtgcggtctg
     1621 gcaaacaaat ccgtgtaaat gtacccgcgc gtttgattgg attcatcgac tatgcaatag
     1681 cattgaacat ccttatgcca aacatccacg ccctcaatct cctcgatact cataccaaag
     1741 agctttttaa caatcgcgaa caagccttgc atcactttag gctgtggaaa ataaggccgt
     1801 aattcctctt gagataagga atacaaatcc tgtcttcttt tttcagacag ataagcaacg
     1861 tcccatgggt ttaccggact cagattccat ttatcctgtg caaacacttc aagctgccta
     1921 aattccgctt tacccttgtc acgagttctg ctgatcaaat catagataaa ttcagtgact
     1981 tgattggtcg aggcagccat cttggtagcc agggataact cagcaaaatt attaaaccct
     2041 aaaagctcgg ctttttcatg gcgcagggac agaatttcat caatcagcgg agtattatca
     2101 aaggttcctg cattagggcc ttgatctgaa gctctggtga tgtaagcacg gtacatttct
     2161 tcacgtaaag ccctgtcttc agcatgagta ataacggcct gaaaacaagg gtattcgagg
     2221 gttaaaacaa aaccatccag ccctttctca tgcgccaact cttttgccgt atttaatgca
     2281 tgctcaggta atcctgccac acgttctgct tcggtaatat ggatagtgta ggcatgggtt
     2341 gcgtccagca cattgttttc gaatttgctg gataattcag ccagtcttga ttgaatggct
     2401 tcaaagcgct ttttattcgc cttggataaa gcaactcccg atagctcaaa atcctgcatg
     2461 ctgtcttcaa tgattttttt ctgggcaggg ttaagggaat gctgatctat cgatttaatc
     2521 gcctcataca agtcttgatt atgcccaatc gcagcatcat aggcagacag caatggcaaa
     2581 cacgtttcat aggattcacg tattgcctca gaatccatca ctgcgtgcat atgtgcaaaa
     2641 ggagaccaaa aacgctccag ttcatcggcg aggctatcta aaggataaat taaattatcc
     2701 caggtatagt ggtgattttc cttaagcagc ctgtcaattt cctcaagatg gttttttaat
     2761 aaagcatcaa gatgagattt gaaatgttca atttttatat ggctaaattg tggcaatcca
     2821 actgtggtcg acatgttata cctttccatc atgtaatttt ttgaaagcat aacattcaat
     2881 tgaattagtc tctatacttt gccggcgctg aaatggcaaa gtacagtaat gaattaaaag
     2941 gtgaacgttg gaatcaattc ctctgcctca agatcctttt ctggcttgga agacaggtgc
     3001 agactctctt ttttttcatt tttgggtgca aaaaaactta atttattgat aacggaagga
     3061 gcaggttttt caatgttatc gacaggtctg tattcatcta acttgttgtt caagtataat
     3121 gaaacgcccc atagcgctag caaaagcact gccccaatca aggggatgac aggatttata
     3181 atacccaaag caggaactga taaagccatt gcaatcacaa cagcgactgc cccgccaacc
     3241 gataaaaagg agcgcaaaat ctcaaactta atgcgataac tgattgagtc ttgatactct
     3301 ttaattatct ttttctggtc ttcattttct tcctggtcga agagttcggc atattccttt
     3361 tgcaactgct gtaatcggcc aatctctatg taagcacgca ggcttgtgtt ggcgacatca
     3421 aagacaaacg caaacaaaga caaataaaca gagagaggtg ccaaagcgcc caccagcacg
     3481 aagcaattga gaatcccgac tgtaaaccaa acgctatcat tggccagctc aaaccaccgt
     3541 cgttgcaggt gggcataaaa tcgatcggac caatccagtg atttttcttt ttcgcccatc
     3601 cattcccctg gaatggtgtg ctttaaaatt aaaaagagat tggtaaataa tctgggggca
     3661 aaaaaacacc agcccaaata agccaaaaat ggattagtgt atttatcaag cagcacgaca
     3721 aaatcacgaa atgcctttga accagtgtct aaattatcca aaagattaat gactcgttta
     3781 cttctgctga taaaaattct ataccaattg gattgagccg ttttctcacg aatggcctgc
     3841 gtcaacgata agcttgtctt gagagattcc ggctccggat tatccaaggc aaaagaatac
     3901 ccggacaatt ccgtcaataa ttctctgtat atggctttat ggcgacgtaa acgcactact
     3961 tcacgtggaa caaccaggta gtgttgatag gtatattcca aaagttctgc cagtttgagt
     4021 gcatcaatca gctgttcctt gatttgctca ggagaactgt aatcaggcgc ggacaggtta
     4081 taccttaagc gctgacacaa tacgctgtat tccatattga gaatgggatg aagacgagct
     4141 attttcgaat aaaaatcagg gcccaattgc ttcagatgcg tgctaaaagt ccaattcaaa
     4201 gaatcagttt taacttgcgt attcagctct ggaaatattt ttaataaatg actaataatc
     4261 ggtgtcatga ttgtttgaaa taaataatcc gcatatcatt gtgtttctaa tccagctttg
     4321 caaaaatttt tgctcctgtc accttatctg ggctgcaaaa attaatcaaa ctccatcatt
     4381 atgtcaaaaa ttaggaaaaa tttaaactta aatttacatt ttgctctaat attgtcatta
     4441 ttttcaacta aaaatgaatg gtcttcttgc cgatttatgc cttctgagcg acaatagtgc
     4501 ctcagttaaa tgcaaaagag taggcatgaa cagttttaca gaattagcta atgcagtacg
     4561 catgttaagc atcgatgcag taaaccaggc tcaatcaggc caccctggca tgcccttggg
     4621 tatggctgat attgccaccg ttttatggaa aaaatttcta aaattcaacc ccaaaaaccc
     4681 gcactggttt aacagagacc gttttgtatt atctaatgga catggttcca tgcttctcta
     4741 ttcgttactg catttgaccg gatacaattt ggatattaac gagcttaaaa atttccgcca
     4801 attgcactca aaaacaccag ggcatccgga acatgggcac actcccggtg tcgaaacaac
     4861 aacagggcca ttaggtcaag gtttggccaa ttcagtcggg atggcattgg cagagcgtgt
     4921 actggccagt cacttcaacc acgatcacta taacctcgtt gatcattata cttatacttt
     4981 tgccggcgat ggatgcctga tggaaggcat ttctcatgaa gcatgttctc tggctggcac
     5041 tcttggtttg ggaaaactca ttgtatttta tgacgacaat gggatatcta ttgatggtaa
     5101 ggtagaatcc tggtttacgg acgataccac gtctcgcttt aaagcctaca actggcaagt
     5161 catagggccc atcgatggcc acgacgcaga tcaaattgag caagccatat cagcagcgcg
     5221 tgagaacacg actcaacctt cattaattat ctgtaaaacc gtcataggac tgggttcctc
     5281 tgtagccggt agcgaaaaag cgcacggttc tccattgagt gcccaagaca taaataatgt
     5341 ccgtgaattt tttaattgga aacatgctcc cttcgaaatt ccagattcga tatacaagca
     5401 atgggatcat agagagcaag gagacaaaga agagcaacaa tggctgcagc tcctgcatga
     5461 ataccggcta caatatccat cggaacacga tgagtttttg cgtcgtgcca atggcgacct
     5521 gcccgatgat tggcaagaaa tatcggctgg attcctaaaa caatgcctga acaatgagaa
     5581 agccatagca acgcgtaaag catcgcagca atgcatagag tttttcgcgc caatattacc
     5641 ggaaatgttt ggcggctcag cggatttgac tggttccaat aataccgact ggtcaggcag
     5701 caaagccatc acggcccata atttttccgg aaattacctt tattatggag tgagggaatt
     5761 tgccatggca gccatcatga atggacttgc tgtgcatgga ggattcattc cctacggcgg
     5821 aacattcctt gttttcgcag attatgcaag aaatgccatc cgtttaagcg ctttaatgaa
     5881 acaaagagtg atatacgtat tgactcatga ttctatcggt ttaggggaag atggcccgac
     5941 acaccaacct atagaacata tcgctatgtt aagaatgacc cctggcatga cagtctggcg
     6001 ccctgctgat ttgatggaaa ccgctgttgc atggcaacaa tcgttagaac atcataacgg
     6061 accttctgct ttgttactct cacgacaaaa tctgcccgct ttagcccatg gcaccgatgc
     6121 ggctgacttg attaagaaag gtggatatat tattgcagat tgtgaaggga agcctgatgc
     6181 tatcctgata gccactggtt cagaggttca actggctata accgcagcag aaaaggcaaa
     6241 agcaagaggt ctccaggtca gggtggtatc catgccctgc gctgagcgat tccttgccca
     6301 gagtgatgct tacaaaaacc aggtattacc tggtcaggta cgcacacgta ttgcgataga
     6361 agcagctagc agtgcctatt ggcaccaatt tgttggcttt gacggcgctg tcattgggct
     6421 ggattgtttt ggggtatcag ctcctgctac agatgctttt aattaccttg gaattacagt
     6481 agaaaaaatt atcaatactc tagatacatt aaccaaacaa acaatggtgt aacccatacc
     6541 cttctgaaat ttcaagaagg cctgtattca gtataactga gtatggaggt taccctaact
     6601 tggagaagca gtatgacaat acgagtcgcg attaatggct atggccgcat tggccgttgc
     6661 attttaagat cgatttttga atacaacagg caaaatgagt ttgaagtcgt tgccattaac
     6721 gatttatctg gcatcgatgt gaccgctcac ctgactcgct acgattcaac tcacggtcct
     6781 tttcgctcag atgttagcat agaaggaaac atgctgattg ttgatgggca tggcattcaa
     6841 gtgattgctg agcgtgatcc ggccaaactg ccatggaaac aattggacat tgatatcgtc
     6901 tttgaatgca ccggtttctt taccagcaga gatgcggcaa tgggtcatat taatgccggc
     6961 gccaaaaaag tattaatatc tgctccggcc aaaggcgttg atacgacagt ggtctatgga
     7021 gttaatcact cggtcatcaa agcgtctgat gtcattgtat ctaacgcttc ctgtacaaca
     7081 aactgcctgg cgccagtagt taaagtatta aatgatgcta taggaattga atccggctta
     7141 gtcaacactg tccatgctta cacaaaagat caaatgcttt tggatggaag ccataaagat
     7201 ttacgacgtg cccgctctgc aacgcagtcc atcattccaa ccaaaactgg cgctgccgca
     7261 gccgttggat tagttttacc tgaactggca gggaaattgg acggatttgc aatgcgagta
     7321 ccggtattaa acgtatccgt tgttgattta actttccagg caaagcgcgc aacttctgtg
     7381 gaagaagtca atgacatcat gcgcaaggct aaaagccgaa ttttacaaat taatgaagat
     7441 cctttagttt cttgtgactt caatcacaac cccgcttccg ctattttcga cacaacagaa
     7501 accaaagtga taggcagttt ggtcaaagta gtagcctggt atgacaatga atggggtttc
     7561 tctaaccgca tgattgatac cgcacaccaa atgatgaact gctaaaggat aaccaatgaa
     7621 cctgattaaa atgagcgaca ttgatctgtc tggcaaaaga gttctgattc gtgaggattt
     7681 aaatgtcccg atcaaagacg gcatgattac cagtgatcaa agattgcaag cggccctgcc
     7741 tacaatcaaa tccgctttgg acaatggcgc agcagtaatc gtattgtccc atcttgggcg
     7801 tccggaagaa ggcaaatacg aaaaaaaatt ctctttggaa cctgttgctg attatttaag
     7861 gaaaaacctg gaatatcccg tccgcttcgt caaagattat ctgagtggtg tggatgtaaa
     7921 acctggggaa ttggtcgttt gcgaaaatgt ccgatttaat cccggtgaaa aagccaatga
     7981 cgaagctttg gcaaaaaaac tggcaaacct ttgtgatgtc tttgtcatgg atgcttttgg
     8041 cacggcacac agggcacaag cttctaccta tggtgtcgct caatatgctc cagttgccgt
     8101 tgctggtcct ctgctaatca gggaacttga agcgttaaac caggtgctca aagcccccaa
     8161 aaaacctatc gtcgccattg ttgggggagc caaagtctca tccaaactca gtttattgaa
     8221 acaattagtg ggtatggtcg atgttctcat ccctggcgga ggtatcgcca atacttttct
     8281 gaaagcccag gggtttgaaa ttggtatttc cctttacgaa cctgatttat tagatgaggc
     8341 tcgccatatc cttattctgg ccaaagaaaa gggttgccaa attcctctac ccaccgacgt
     8401 tgtagtaggc aaaacattta gtgaaacctg ccctgctttc aacaagtctc tgtctaatgt
     8461 ggctgaggac gatatgattc ttgacattgg accagaaacc ataagagatt atgtagatct
     8521 catccatgaa gccaatacca tcatctggaa tggtccggtt ggcgtattcg aattcccgca
     8581 gtttgcttat ggaacaagag ccatcgccat agctattgcc gagagtgatg ccttttctat
     8641 tgctggtggt ggtgatacct tggccgctgt tgacctttat gatttgaatc aacaaatttc
     8701 ttatatttcc acaggaggtg gggcatttct ggaatgtctg gaaggaaaaa ccttgcctgc
     8761 tgttgctatt ttgcaggagc gcgcaaaaca tgttaagacg aactaagatt gttgccactt
     8821 taggcccagc cagtaaggaa cctgaaattc tgcgatccat gctggctgct ggagtcaacg
     8881 tagtacgaat taacttttct catgctgatt cctccgcgct gcaattaatt gcgctggttc
     8941 gtaaaatcgc agatgaatta aaccacccgg ttgcagtcat ggctgactta caagggccta
     9001 aaataagagt cggccgtttt caaaacaaat ccatcacttt aattgatggg caaaatttta
     9061 cattggattg tatggcccca gataccctgg gtgatataaa tggcgtatca gttgcttatc
     9121 ctaatttggc cagtgaactc agtattggtg atcatctgct gattaatgat gggttaattg
     9181 aattggaagt tatcgagatt tccgggtcaa aaattcattg caaggtagtg gaaggtggag
     9241 tactcactga tttgaaaggt ttgaatcgaa aaggtggcgg gttagcagcc agaactttaa
     9301 cagaaaaaga tagaaatgat ttgcgtacag caattgaagc agaagtggac tacatcagtt
     9361 tatcttttgt aaaagacgcg gaagatatcc ggcaagcaag agcgctaatg aaagactatg
     9421 gggccaaaat aacccctatt atcgctaaaa ttgagcgcat ggaagccttg gatcacttaa
     9481 ctgatattat tcgcgaagca gacgccatca tggtggccag aggagattta ggcgttgaag
     9541 tgggtgctgc tgaagttccc gctattcaaa aacacatcat agaacaaacg cgacttttag
     9601 ataaagtggt gattaccgca acacaaatga tggaatccat gataagcaat cctcagccta
     9661 cccgtgccga ggtttctgat gtagccaacg cgattctgga tggaactgat gctgtgatgc
     9721 tttcagctga aacagcaagc ggcctttttc cagtaaaagt cattaccatg gtcaataaaa
     9781 tatgtttaag cgctgagaag catgccagct ttttttatca tagtgatccg gaaacctgtc
     9841 attaccaacg ggctgaccag gcaatcgcta tggctaccat gcataccgcc aatcattttc
     9901 ccattcaggc cattatcaca ctgaccgaat ccggagacac ggctttatgg gtatcccgac
     9961 accacagcac cgttcctatt tttgctatat cagccaataa gagaaccatt ggtcgtttaa
    10021 gccttgtaaa caatgtcttt cctatctaca ttgattttca tcaattcaac cctgaaggat
    10081 tgaatcaaca aattttgcat gaattaatca aatcagggca ccttgaaaag agaggatatg
    10141 ttttattaac acgtggaacc caaattggaa tgcctggcgg taccaactgc atggaaatta
    10201 tacctgttgt ctaagttcaa gatatcctat taattaaagc aaggttcttt cataaaagac
    10261 aaaaaatgat tattttaatg tcactttaat caaaatatca cctctatgct actattaagc
    10321 atagaggtga tcaaaatgcc ggataaaaaa tcatcagaaa ctctgttatc acagcaaata
    10381 aatgaagtaa gaggtttgct gctgcaagcc cttaaaactc aattagatga tctggtagct
    10441 gaaaaactca ttccacaacc aagtggcgtt ccttttgttg attttgagaa agatccacca
    10501 caaattgcaa ctctaaaaaa agtcattaat tgtctttatc atgcagagga agcgtttaaa
    10561 agctgggaaa gcattgatgc cagtacgcta ttgggtaaag ccaaagcagc gccaaaatta
    10621 attcgtgcgc taacccagat atacaaatct ttgggcttgc ttgatgaggc aagccctgaa
    10681 ataagaggtg tcatagcaga taattaccat ttacttgaac ctctatttac tcaagcgtat
    10741 gccatcgtgc aagagtctgg ctggttatca gaattcatgg aaatggatgt gaccgataaa
    10801 gcatccaaag tgatcttcca agggatggat ttattaggtc ccgatatcga taaatggcat
    10861 gatacgcatc ctcttgttga cgcattttca aagatatcaa aactggtaga aaccatatcc
    10921 aaactacaag aaaaggacat gccaggaaaa gagaaagagc aagcggtaga attggttcac
    10981 gccttacttg atgacctgga caataaccct tttatcagca aattaagtat cagtgatttt
    11041 gaagactcca aagccataaa ggatttattg gaatggttta agaacataca agacgatggt
    11101 tttgatttta gcaagaaatc cattaagcaa tatgtttcct gggcaaatca ctatttaccc
    11161 acattaatcg ccttcactga tcagctggaa cggcaaaact atttaaaacc aggcagctta
    11221 tccgccaatt tgtgttctca aatagaacag ttgggtaaac aagtgaatga catactgtct
    11281 gaacctggtt ttggtattac tgagcgagtt gtaacaatag actcattgca aaggcaaaga
    11341 gagcaaaaaa ttcataatgc tcaggtcaaa agtgtgcaag ttatcatggc tactgaaaaa
    11401 caacaggcgg ccactgctga tttttatcgt atcctggaag cctataaggg catgaatctt
    11461 tcacaaataa cagaaccgga tcgcagagta ctaagacaat tatatcccca aatacaaaca
    11521 gcactggccc atgccagttt ggatcttgaa aatcagttaa cctctgtttt aaacactacc
    11581 ggccctgaaa aaaccccaac caaacccaaa tcatggtggg aattagccaa aggtggttta
    11641 ggttatgtag ccagttatgt ggtcaattac aacgttgata aaaccctggg aaccagagta
    11701 tctgttgatc gttttctgtc aagccaaatt gcgtccgagc aatttaagat cctggttgct
    11761 gaaaaagccc gtgaaaaatt aagaaccgga tcagataaaa taaaagaaac tgcactgcaa
    11821 tatcaagctg aagctcgtgt caatgcaata aaatcaaccc tgagaaagca acccgctcag
    11881 atggaagctc ctccatccca atttgttgca gtaaaaccct caaaactgct caatcttcgt
    11941 ggaacactta cagccattca ggaaatgcaa ttatcgacta cagtaaacgg cacaagaaca
    12001 agtgtcgata ctctgattca tcgccatttg ccagaaaaat atcaagtctg tttttcaact
    12061 cttccgtatc aagtcaataa aaatgatcct gaattggtga aacaaattaa aaccgttgaa
    12121 aataatttgt ttcaattaga aaaagcgctt aaagattttg aaaaaatcga tttgagctat
    12181 gggattatga ttcgattgca ttattttatc gcaatcgctt ctgcagcctc caaactcaaa
    12241 tcaagtgtta aagaactttc ccctgagggt caaaaagcgc tagccccact attacaacaa
    12301 attatgacat taggctcatc cttctcagac accaattata cagctaatga tttgtccgcc
    12361 ttgcaacaat taaaaatgga aggccagctg agacctaaaa gcccatccgt agcatctccg
    12421 gatacaatag gaacattacc tgaagaaact gtaaagagaa ctgcaaaaaa accaaaggaa
    12481 aaaacgactg aattattgga ttatgctgat aaaatcagtc atgctcgagc cttgctatta
    12541 gataagttta aaaccaccct gtctcctgca ctagctgacg atttaaatcc acaaaaaaaa
    12601 ggcgttccat ttgttcatct tgatcaagac ccgccacaaa tcgcggcaat aaaaaaaatt
    12661 atcaacagta tgtatcatgc ggagtgtgct cttaagacct ggcatggcat agataccagc
    12721 actacattgg gcaaagttgc tgcagcacat caaggcgtag cggcattatc gcaagtatac
    12781 aaatcattta cgttgttcac cgaggatttc tcagacattc acaatttaat tcgtgagaac
    12841 cacgatttaa tagcgcctgt gattatcgaa gcaaacaagc tggttaatca gtatggatgg
    12901 gcacgtcaat tcagtgagtt ggatatagca caacaactgg gaagcatttt aggtcagggt
    12961 attaatacag ttcaacctaa aacggatgaa gtaacacaaa ccgcttctct ggttaaatta
    13021 ttttcagaaa ttccaaggtt attgaacaat atttcaagtt cattggatca aaaatctgag
    13081 aaatcaattg atgagttacg aatcagcaac aaaaaaattg aagccatagg cgctgtattt
    13141 gaattgtttt ttgaagaaca tgcttactta atgagcatgt ttaaaggatt cagcgctatc
    13201 tcgggtctta tcgaattaaa taaaaaaatt caacgcgaag gaaccaacct gcaggaagcc
    13261 actattcgac aataccaggc atggttaaaa gaagggtatc ccaatttgat gatgacgctt
    13321 gatgaaattg agactcgtca ctacttaacc cccggcctgc tatcagcacc tattgccctg
    13381 gaattagacc aaattaacga caagcttaat gaaattattg aaaccaaacc tgattttaaa
    13441 ttacaaaaaa ttcctctttc attctatatg ggagataagc gcatagagcg cttaatgact
    13501 aaaaaaacgg aacactgcct cgcattgctg caaatagaag agcaaaaaaa agcagtgaac
    13561 acttttttcg ggatacttaa acactattca ggccaaccgc tgtcaaacat aaagccaaat
    13621 gagctcgctc aattgcgtct tgcttttgtt gagattcaat ccgcaatggc aaatagtaat
    13681 ttggaattaa gcaatgattt cgttgctgct ttaaatcagc ttgaaacgtc cccacctgaa
    13741 aaaacaaaga aaatcaaggt gacagtctct caattgttaa aggtgcagcc tgctgttgaa
    13801 aactatttaa acgaacacaa aaaatcctgt gaattaaaaa tagcagtcat tgacaaagcc
    13861 atcgagcacg tcaaagacaa atcgttcgag ctgaaatctg gtgcatcaaa agaaatcaat
    13921 atctctcagc tgcgcagaac atttttagaa actgaaagca agaagcctgt tgtaggtcct
    13981 ggggaattaa aacgaacatc agtctcatct cttaataccg ttcgtggtaa tttggcttac
    14041 attcaagaat taaaaatgtc atccaccgta ggcatgatgc gagagcaatt ttctgctctc
    14101 accagagata atttctccac tcgagtgcaa aaatatttaa tcaaacctca agacaagccg
    14161 ttgcatatga taaatgacaa cgatccgctc atggtaaggc aaattaaaga agtcgaaaac
    14221 ggtttatatc acttggaaac cgctttaaag caatttgaac aaatgaaaaa gagtgacagc
    14281 ctggtatcac aaaccaaagc actgctagaa attgcaaacc atgctctcca attaaaaatg
    14341 gcggtggaac gattttcacc tgagttaaaa gagcattatg gcccattagt tcggactgtg
    14401 tttagtttca gtaataaaat tcaatccatc aattacaata aagaagattg ggctgatctt
    14461 cagtttattc ttaatagagc ccataaagaa ttgttaaaaa gaaaaacacc aagagaccat
    14521 cgtattgaaa aagctttttt tgaggccgga attcgtgaag tacaaatcga tcgtaatgaa
    14581 tcggtaggaa aaaaaactgc aaaactgggt gtaaaatacg cccatctggc atcccctcag
    14641 ttagagaaag gaagagccta tttaagttcc agatacaaaa gtgcgtttgg tcaacagcct
    14701 aaggttattc gttcgtttac ccgagaacaa ttagcgaatg aagaatttat ggacgctgaa
    14761 attagcagat tgaaaagaac attagaagag cagtatggct ttaatattgc aacgatcaaa
    14821 gtttttcttg ctttattaga acaaattcaa cgggctgggg ctcaggctgc tgaggtagct
    14881 ggtatggtca ataccttggt tacttatgac ttccccaaga taaaagaaaa tgcgtatagg
    14941 gatttactta cgaaattatc tcaagaagaa gactacctga atttaaaacc aggcacatta
    15001 attaatccag ccatggctgc agtcaaccaa ttattcttat ctgctgcctt ggagttagac
    15061 atgcctttta gtaaaaagtt atctattctg gacgatacca cttatgtcaa tattctcata
    15121 agacaaaccg aaagcgatct gcaaacatta aagaaaaagc tatccgaaga tccctcgaat
    15181 catgaaatag catttgaaat tgagattaag aacgataaat tagctttcct caggcaacag
    15241 atggtgttgc ttgaagataa aaaccccaat caaaccagaa atgtcttatt ggacatgcag
    15301 ttcgaggtgt cattacgtaa tcatcttgtt tatacgacgc ttaaagctcc tattgcccag
    15361 gaatatgaac aaattgccag agaatattac aaaaaaaata aaaatcgctt tctgtatgcg
    15421 gatgactgtt caaaagagtt gctgcaagga ttgaaagaat ttgaaaaaaa acatttgcct
    15481 gatcatttga tagtttttga agcctacgac aggctccgcc tattcagcgc aaaacttccc
    15541 cctaaaaatc aggatgtcaa agagtatatt gagaacatca atagagaatt acaaaataaa
    15601 gatatcccta tagagcaacg tgctttaaaa gttaaatcat tacctaatga ctctgtcttt
    15661 attgaaaaat taagttcagc agataaaggt actcatttcc taagaaaatt catgcagttc
    15721 attacaatca ttacagcaag catcaccgaa gcaataaaaa ccgggggcag tttgatttat
    15781 atttatcgcc aaaaacaaat ggaacaatcg ataaagaata tagaaaaatc gataagcttt
    15841 aaagaggagc tccataccat aagagatcct gatagccctt cgccacgcaa gggagaagga
    15901 aaagaaggtg aaatcgaatt taccaaattg taagactcaa taggctatat tggtaaaaag
    15961 tgattctaca ctctgaactt accccgtgaa cagtgtgtaa atagcaatgg caaaaatatt
    16021 catgttaaat gcctaaggtt gttttaaaat cataaagtaa gcatcgacat aatcgggtaa
    16081 atctcgagta gccctgtaca aagcgtcctt tcctgcaaag cttcctgcca aataatcaat
    16141 ttgatataaa ttattattaa aagcccctcc agtatcagcc aggatgccca ttttagtcat
    16201 tgtgcgtcct gatttgtctt gatactgaat cattagtaat ttccctaaac caaactcagc
    16261 caaatcagct gcaaaggtca ccttggggtt aaccgtaatt ttgtattcag cgtccttacc
    16321 ataaccttta attccatcga cttccttgaa ataccaaaat cgctcctgct tatagggagt
    16381 tttagttcga tcataagcaa tattattact acgatggaca ttaaatattt tcttgccagc
    16441 agtcggtcca aaatccgcga caacggtacc ttgcagcaaa gccgcctcca aatcatctcg
    16501 acttaaataa gccaattcgc taaccggttg atttttaaga gcaccggata atatcgcttg
    16561 cttaccatat ctgaaacgaa ttagtcctgg tttggtattt gcttcatcca gagttaaatt
    16621 ctgctcgtct tcgggcaagg catacagagg atagggtttc gcggagcttg gtttggtact
    16681 ggcaccagcc aaatgcacaa aatatttagt catcaatatc ttatctttgg gtatattttg
    16741 taataatggt ttattggcag ataattgttt ggcttggctt aagtcaggat accatcgaat
    16801 aaagtcgaaa tgttgtttta caaagtccgg gttatttaat tttttttggt tttgacaaat
    16861 aaattccaag gtttttttca ctctgtctaa agaaatctta atgacttttc catcgtgaat
    16921 cacttgggga tcataatttt tgcctttatt taagtaggct aaagtatgct ttgctgtact
    16981 gcataattca gttcccttaa tgtcatatac tccattcaag gcaggttggg atggaaagaa
    17041 ttttggcgca gcccataatg gagaaataaa cagtactgct gaaaataatg tcgctattcg
    17101 catttactct gttgtttcct ttttatcttc tgcttttcga gacaatccta atttttcttt
    17161 taaacgagct acagcatccg gatcatattg ctttggtgct ttatgtttga ctactacgga
    17221 tggtgacttg gcggattgca ttgccacatt ttgtgaggca taggtagagc ttctgagagg
    17281 atatattggc aaaagaggct caggatgaga gtcaaacgaa ttcattggct taacgctgga
    17341 aactgtactt ggctgattat ttacatatga atgattagcc gcttttgcat tcacttgttt
    17401 gcgagcattt ttaacactct tttccacgcg ttttttaatt ttcatggaag cattctccgc
    17461 ttcttcctca gtaacctccg ctactggatt tccatgcaaa tcgatacgga cttcacgagc
    17521 tttcaggcaa gccagataat caagacgacg ggtaaaaaga acaacagctt cccttaattt
    17581 gcttttagaa actccaactt gctctgcttt ttctgcatgc tgcaatatat cagacataat
    17641 accaatcttt aatggccgaa tacgcaaaga attatcgaat gcctcaggga aattagccgc
    17701 aagccataat aatgcgtcag atcgcgcgcg cttggattga tttttttgtg ctttattgat
    17761 gacagcggtt cgtgggtgca gcgcctgctt tctcattctg gtacccttat tgtgtttagt
    17821 ctattggcat gaaaattatc aaaatcattt gatttgcaaa ggtctgttga ccttgaatgt
    17881 atgatagtca tatcttgaag tcagatggtg taaattatgc tactagttac tcgaggaatg
    17941 agcgcacaat gtacaatgtt tattggttgt ttgcaagcct tctttattat ttccattaag
    18001 ccgaatgtct tttggtttgt atccaattag caaaatacat tatgatatgt ctataattaa
    18061 cttaaatcag atagaatagc gcagaggatg cttgatagat tgttaaataa aggaacctta
    18121 tcgctcaata atgtattaat ggtgattaca aaatgaggat aatactttca attcttgcta
    18181 tattcatgcc gtggttggtt ttattgatta aagacaatcc aggcggagct gtcgttgcct
    18241 tgattatgca agcgacacta attggatggc cttttgcttc aatctgggca tggcgggcag
    18301 tcaacccact tactccggcg tctcaatcca aacaataagt atcccaatcc tgtatttatt
    18361 caacatataa ctaaatcagg acttacgaaa aatgccaaat cgaggcaaaa aattttttta
    18421 atgagggagt ttagataggc tcaatgaccg aattaaaaat ttttttataa agagattggg
    18481 gtttttcgta aatcatgtaa ataattcgga gtgataaatg cgaacacgtg tcattgttaa
    18541 tggtgccaat ggaaaaatgg ggatactagc ctgtgaaaca ttggaaaacc atgaacagtt
    18601 tgaagtagta gccaaactaa gcagacagga caatcttggg caaagtatac tggacaccaa
    18661 agcacaaata gttgttgatt taacaagagc agactgtgtc tatgaaaact ctttaaccat
    18721 tattaatcac ggcgctcgac cggttattgg aacatcaggg ttggttgaaa cacaaatcaa
    18781 tgagctcacc aaactttgtg aaataaaaca aataggtgga attattgcac ctaacttttc
    18841 tctcggcgcc attttaatga tgatgcttgc cacaaaagct tctgaatatt tttcagaggt
    18901 tgaaatcatt gaaggccatc atcaacaaaa attggatgca ccatcaggga ctgctttaaa
    18961 aactgcagaa atgatagctg cggcgcgcaa aaagcctaaa aacaaacttc cattaaaaga
    19021 attaacccct ggggcccgag gcggctctca tcatgatatt aatattcatt cccttagact
    19081 gcctggattg cttgctcggc aagaagtcct ctttggcaat ataggcgaga cactctccat
    19141 tacacataac agtatagatc gccgctgttt tatgccggga attgtgctgg cctgtcaaaa
    19201 agtattgaat ttaactaatc tggtttatgg tctggaacac ttgctttaaa ttgcaaaaac
    19261 caggtcgacc cgtattacta tcgggcatac gggtctcctt ttcgcatttc accccttgat
    19321 atggaacgtt tcccaactca attttttaaa cattacatat aaaccaatcc actgttttct
    19381 cttttggaac tctcaaacaa agaagaccat tctaaaatcg cttcttctgc cagtgtttgt
    19441 aaagtggcct ctgatgacag gggctgtaat aatttcctaa tacgatcata gactgaattg
    19501 gttgctgtag ttcttgatga gtcgctttct ggccgattta aaacggcagc aaatattttt
    19561 ccgatacgaa gaacattatc attgccagaa ttttcatcat gcattacttc acgaatattg
    19621 agtattccag taggtgttga gctcacatct ttatcagaga accaacgtaa tttccaggag
    19681 tctttgcttg atttttgaaa tctgcgctcc tcattaatta aagcgcttag cacatcataa
    19741 agcattgtac acattttttc gcccagctgt ttggcaatta acaaacaatg caattcgttt
    19801 tctggtagaa aaaaggaacg caaatccttg ctaatattct tcacttcatt atcagtggct
    19861 aatcggtcat cataggctaa tgctttctca gtgcccaagc gctcgttaat ggcctcagcg
    19921 atatcgtttg gccaatacca atcaggcgtt ttgattccct ctgaacctgg cgcattttgc
    19981 cctgcaagct cgtgttgatg tctggagata taaagatcta caacaatatt gatgaaattg
    20041 actcgttctt gtttttcttt gggaattcca aatttaggcc aatttccata ttttgcttta
    20101 tacaaaatca tcgcatcagt atgcaataac tctactgctt ttctgtcttt accactgaca
    20161 caggagccat agctatagcc gcccatgttt agaataatca gttcttccaa agagctaaga
    20221 aataattccc tgccatcgta atcccaaaat gttgcagagc ctaaaggcga ctctaaaacg
    20281 gccttataat catcgattag cgcctgcaat ccaggcttgg aagacgcata tttctgcgct
    20341 gtttttaata aaaactcgct gtctgtatcc gttgcctgag tataataata acgcttggca
    20401 atattaaaag gatgattatg ctggaatatt tcatgcctgc gtttgctccg cgtaaccgct
    20461 tctcgggcaa ttttatacaa atccagatcc ggaggtaatt ccggtaaaaa gtctgtaacc
    20521 accgttggga tataatctac tgcatggata gggctaatta atgtttgcaa taacaaaggc
    20581 tgctgaggtt tggcaaattc catcacttta accaggttag agtcaacatg acgttgctgg
    20641 acatcctcag gaaattttaa cgaatcacga ctggcaacat ggcttgagcg atacctcttg
    20701 tcataaaata aagtttcttt accctcacca tcgattaaat ataaactgtg cgcaccataa
    20761 ttggctggag caggcaaagt tcggtgccgg cttgataaat aagatacgac atcctcaacc
    20821 ttttctgcat ttttgagagc atattccaaa aagtattgtt gtttctcgga taaaacccaa
    20881 taccactgtg gaagcttggg cactaggctc aaagtcgatg cgtattgatc attcctggcc
    20941 tgctcaacca taaatttttt aaactctttt aagctggatt taatctcatg tggagtagca
    21001 gacaagacgc gaatcatggc ttgctgagca gggcttaatt cattgaacca ggaaggataa
    21061 ggcggtgagt tggtatgtat ttgatttaat tccgtggtca aattcagtga cctttttgca
    21121 atatctcccc aattgccaaa taatgtatta aagtgctgca gcgctttttt aggatcaaca
    21181 gaacttaaat tcagattaca ataataagct tgttgatagg gcggcagatt tttaaaccag
    21241 gcaggtgttt taggaaaatt atattctttt aattgtttta attcagttaa ccattgttca
    21301 taaacgggag aaagggattc atcatattgc aaaacatact ttgtctcact gcccatttca
    21361 ataggcatta tggtagcaat atcgctacga ccccgactca ttaagacata ctgctctgct
    21421 tcattaaggc tggctatcgc ctctttgact gccttgcctt taggccattt ccacgcgatg
    21481 gcaatagctt caaccaattt ggccgtaaat cgttcgagct caagatgcaa ctcttctaat
    21541 gtcaattttt ccaggtcaca tgaagccttg cattctgcgg cataagtact taaggttgca
    21601 accgtcagag aagtatgttt tggatcgagg gggtaagctt ttaagataaa tgaaaaggca
    21661 tcatgaaggc tgtctattcg gcgagcgatg cttttacgat aatctggctg ctctttgaaa
    21721 tactctactt tgtaaccagg tagaagtcgc tctagtgtat caaatacctg aggagataat
    21781 attgtatttt ccaaaacacg taaaattata ccttttccca tagcttaatt cccttactgc
    21841 cttaatttcc tcattattgt ataaataatt caaagtgaca atgttttgaa ttaacaattg
    21901 aattatatat ttaccctaag atatcaggaa gttaattgcc ttaatagcat tacttcatgc
    21961 taaactactt tttttggagg aatagtaaaa ggatagctat gaaaagtata gaggatttta
    22021 caggtttttc agaatccggg attgatgatg ccattcaaaa tgccttaaaa aaagcaggta
    22081 atccaactca ttttgaagtc gttgaaacac taggttcaca agataggcaa tcagatcgac
    22141 gatatcaggt aacacttaaa acactgagtg attaatcatt aagtaacggc aatcattggc
    22201 tagaaataaa tgacaaaaat ttaatgtcag ttctgtttct ataactatta tctagctgct
    22261 gcctttcaat acaaggagtt taaaaatggg atacaatgtg cctcactata ttggtggaca
    22321 acttattaat gaaacggata ctgaagctca agccatttat aatcctgctc tgggtgaaat
    22381 tattggccaa gtccattttg cgtcgaaggc aacctgtgat agagctgtgg ctattgccaa
    22441 agcggcagga aatgaatggt cgcaaacccc tgctataaaa agagcgcgag ttttattccg
    22501 atttagagag ttgctggaaa aataccaaat ggatctggca cgaatagtca ctcgtgaaca
    22561 cggcaaaacg ctggatgatg cgaaaggatc ggtggccaga ggcattgagg tagtggagct
    22621 gcactgtggg ctcgtcaacg agctaaaggg ggatttttct gctgaagtgg ccagccatat
    22681 tgattgccat acttttcgac aacctttggg agtatgtgcc ggagtatctc catttaactt
    22741 tcctgtcatg gtacccattt ggatgatgat tcctgccatt gcttgtggaa atacctttat
    22801 tcttaaaccc tccgagcaag atccctctgc gcctgtccgt ttattggagc tgttaagtga
    22861 tgccggtttg cctccgggcg ttgccaattg tatccagggt aataaaacga ctgtcgagca
    22921 tttactggct catccggatg tagctgcctt tactgcggtc gcctccactc ctgttgccga
    22981 atacatctat accactgcag cagcctatgg aaagcgggca cacacttttg gcggagctaa
    23041 aaatcactgt gtggtgatgc ctgatgccga tttagaccag gcagctaatg ctatcgtcgg
    23101 agcagcatat ggttcggcag gagaacgttg tatggcttta tctgtcgttg taacggttgg
    23161 tcaacatacc gctgatgcct tgatcaataa aatttccccc ctcattcgag ccattcgcgt
    23221 agacgccgga gatgttgagg gcactgatat gggtcctctt gtcagccagg ctcatcgaca
    23281 aagagttttg gccgctattg aaaaaggggt taatgaaggt gcccgcttga ttatcgatgg
    23341 cagaaccttt caacatcctc aatacccaca gggtttttac ctgggaccct gcttgtttga
    23401 tgaagtaacc gaagaaatgt ctgtctatca aaatgaaatt tttggacctg tgctggttat
    23461 cgtgcgagtc aaccattttg aagaggctct tgctttagtc aataaacatc aatacggaaa
    23521 tggcactgct attttcactc gggatggctt tacagcacgt gagtatagcc agcgagttca
    23581 agcaggcctg gtcggcatta atattccaat acccgttccc attgccaacc atcctttcgg
    23641 aggctggaag cgctctgtgt ttggagatac caacatgcac ggagaagaaa gtattcattt
    23701 ctatactcga cgcaaaacgg ttaccagcaa atggccaata accgaattaa aggatagcag
    23761 ctttgccatg cccactcatg attaatcatg cttactacgg agaaatatat ggctaacatt
    23821 ggatttgtag gattgggtca tatgggtttt ccaatggcca ttaatctttt gaaagctggt
    23881 catagtgtca tgggctatga tttgcaacaa tcagcacttg agcattttgc tgaacaagga
    23941 ggagctattg ctcacaactt gcaggaactg gcgaaagaca aagatgtcat tgttactatg
    24001 ttacaaaccg ggcagcaagt tttacacgta tgccttggtg tagatggtat attcagtcag
    24061 ttaaatgctg gctcactgta catcgattgc tctacaattg atgtaaaaag ctctcgagaa
    24121 acacatcaaa tagcgaaaga aaatcatctt ctggtcgtcg atgctcccgt ctctggcggc
    24181 gtagcgggag ccgctgcagc cacgttaaca ttcatggtag gtggtgaagc ggaggcttat
    24241 aatgcggcgc atcccattct ctctgctatg ggaaaaaaaa tcatccatac aggtggtgca
    24301 ggcagtgggc aagcggccaa aatttgcaat aacatgatat tgggtaccac gatgatcgcc
    24361 atatcagaag cgttcattct ggcgaaagaa ctagggctgt ctgatggaaa attatttgag
    24421 gtggtaaaca attcatcggg tcaatgttgg gctatgagca aatacgtccc tgtaccagga
    24481 atattggaaa atgttcctgc aaataatgat tataagccag ggtttgcagc aaacatgatg
    24541 ttgaaggatt tgttattgag tcaaaatact gcgaaatcag tacacattga aacacccctt
    24601 ggctccaaaa caactgaaat ttatcaacaa tttgtcgatc agggacttgg tgatatcgat
    24661 ttttcagcca ttattaaaca cattggcaaa acacaagagg tgtagcatgc ttcccgttca
    24721 tctaaggcac cctcatttaa cacctacaga agaatcttta tctgaatttt ggtctgaaat
    24781 tgccgcgcaa accattgact ggatacaacc ttggagtatt accctgcagg gaggattaca
    24841 caaaggcgat gtgaaatggt ttcaaggcgg cttgctcaat gttagcgcta attgtctgga
    24901 taggcattta ccccataaag cgaatcaaac ggccatcatt tgggaaggtg atgatgaaaa
    24961 ccaaaataaa accctgactt ttgcgcagct ttattctgaa gtatgcaaaa tgagcaatgt
    25021 gttaaaaagc ctaaatgttc gcaaagggga tactgtaggc atttatttgc ccatgattcc
    25081 tgaagcggcc attgccatgc tggcttgtgc tcgtatagga gctattcata ccgttgtgtt
    25141 tgccgggttt tcggcccatg cattgcagca gcgattaata gcctcttcct gtaaatgttt
    25201 aatcactgcg gatgcgtttc aacgcggtgg aaaaaccatc cctttaaaga aacaggctga
    25261 tgaagccagc gtggacttaa atatcaccaa gctggttgtt aaaaatttaa ataccccaac
    25321 ggcactaaat aagaacaaag aacattggtg gcatgagcta aaacaaaccg tatcagatca
    25381 atgcatgccg gaaccaatga acgccgaaga tccactgttt attctctata cttcaggaag
    25441 cacagggcag ccgaaagggg tggtacatac cacaggcggc tatttagtgc aggccgctta
    25501 tacgcatcaa ctgatatttg cttgtcagga taatgaagta ttttggtgta ctgctgatgt
    25561 cggttggatt acagggcata gttatgtcgt ctatggcccc ctctgcaatg gcattaccac
    25621 attgatgttt gaaggaatac ctacatggcc tgacgcagca agaaactggc gcatcattga
    25681 taagcatcaa gtcaatgtgt tctacacagc tcccacggcc atccgctccc tgatgagaac
    25741 tggcgatcaa tggctaaact caagctcaag aagttctctt cgtttactag gttcagtggg
    25801 tgagccaatt aacccggaag catggaattg gtatcaccaa aaagttggac aagggaaatg
    25861 cccgatagtg gatacatggt ggcaaactga aactggagcc attatgatta gtcccagagc
    25921 cagtgatgaa gtgaccaaac caggctctgc tcgtaagcct attcctggga tagtaccact
    25981 cctgttaaat gagcaaggac atgaaattaa tggcgctgga gaaggccttt tggctatcaa
    26041 atacccatgg ccttctatgg caagaaccat tgcaggagat caccaaagat actgtaacac
    26101 ttatttatcc aacggctatt acatcactgg tgatggagcc aaacgagatg aggatggaga
    26161 ttattggatt acaggtcgaa ttgatgacgt acttaatgtt tccggccatc gattgggtac
    26221 tgctgaaatt gaatcagcat tggtcagcca tcctaaggtt gctgaagctg gcgttgtagg
    26281 tattcctcat gatttaaaag gtcaaggaat attcgcctat gtcattctga agcagggaaa
    26341 taagccggat gccaagctcc aaactgaatt gatggagcga gtcaaagatg aaatcagtgc
    26401 tatcgccaaa ccagatgtca ttcaatttgt aaatgatttg ccgaaaaccc gttcaggtaa
    26461 aattatgcgc cgaatactaa gaaaaattgc ttgtaaagag gtgtcgcaca ttgatgagct
    26521 gggtgattta acaactcttg ctaaccctca gatcgttgag gagctcatga aaaatatttt
    26581 gaagcggtaa aaagaactgt cgagcttcac aaagtttatt ctgatagcaa ataaataaac
    26641 tatgtgaagc ctgacaacca gttcaaataa tatcaaatca atttcatcag gctctgacag
    26701 agagcggttg cattgacaat gaaaaatctt tgacaatttt ttcagactcg gtgggtacag
    26761 gaaatagtcg ccatcttccc gcgtataaat tggctccgcc caaagtgcat aaagtggcta
    26821 aagcactcgc taaatcagca aacacttgtt tccaaccacg atgtacttct aaaacaggta
    26881 tgactgcatt aatggcacgg acgcaatctt gatgaaaatc cttgcatttc acattcttgg
    26941 gcagctgaga gtttttgaag cgctcttcgg cccttaataa attattgtaa aatgttctcg
    27001 ctatgggtgc cgcgcgttta taattttcat cgccaaccgc tttggtttcg agcgttttaa
    27061 ccatactttc aatagttttc aagtggtcgg aaaatttaat ttcggcaaga atattcaagg
    27121 tttcttttgt tgcagcgatt tggtcagtaa tacgcttgct cattacatca gcttgcctgt
    27181 taatttcgcg aactttatct gtcagagcca ttttgatatc actatgatac tcaccagata
    27241 aaagttgaag ctgttcctga gccttaagca aatctggttt gttgcccacc aaatttcgaa
    27301 ccgattgaat taaatcctgt tttctggcaa tcacttcttc aactgtttgg cacctgttaa
    27361 aacttatcgg aaattcgctg atttgttttt catagctttt tactacttct tcagcattta
    27421 ttttaatgat taattgatca gctgtttcat ttatttcttg ttttttcgct tgcagtgctc
    27481 ttactatgga tggttgcaag tcattacaat ccagaatttt ttcagcactt aacaaagctt
    27541 gattaggttt aaccagatta tcaagttgtt gcaataaatt attacgctgc cattctactg
    27601 cggaagcgga gcttatatca tcaaagcgaa ttacaaaatt atccattctt ttttcaacag
    27661 aattgataac cttttgcgcc tcatcaagaa gccgtttttg caataattca gcccgcaaat
    27721 taatcttttc aacctgagca taatagacca tttttatttt tggattggcg atgaattgct
    27781 cgcccaaaag atcttccgtt ttgaccagtg tgtgaagctt tttgttcaac agaatacgtt
    27841 gggattcaat agctactatg gtatccgcat cattaaagtc gattgggaaa ttttttattt
    27901 gcctgacatg ctcatctata tccgtttcat acttcctgag aaaatctgaa cgtcgattag
    27961 ctgcggcgtc tatcattcgg agcttacgtt ctaaagcctt ctggacttcc ggatgtccat
    28021 caggtaaatc cagtatttgc aacgcatttt gcaattcccc agaatgaact attttcttaa
    28081 gttgacctct gaactcttct gcattgagat taacgtgtgc tactgtttta aatccctcaa
    28141 aactaaatgg aaaagaattg attcgctgaa caatatctga aatcactctt ttagcatcgt
    28201 cgactcgttg ttgccttaat acattatcaa tttctggaat ttttatcaca ggttgtggct
    28261 tggataagga ctcaaaccat gatttgaatt cgcctgaatc ttttaaagcg attttttttg
    28321 cgtattcttc taaagatatg ccaaataaaa cattaatttt ttgagatgag aaaacaaata
    28381 aagcttttgc cacttctgga ttaattcggc ttaatctgaa caaattttca aagaattcat
    28441 ccggatcttt aaacgttgcg ctggcaagaa cgacatcaaa aaactcctca tcaattggcg
    28501 atgtgtcttt gctttttaag tactcaatca ataaatccgg taaagtcttt agtgtgaaat
    28561 agttagcaat cgcttttgca aaaagagctt ttggcggaaa taatgtctct attgattttt
    28621 tggagaaaac attggctata aacaatgctc ttaaccctga tgctcttgtt aaactagagg
    28681 atgctccacc aaagtaaaag ttaattatcc cggattcaat ttcttgtttc gctttttcag
    28741 gcatgtaagc taactgattg attttttgct gtattgcaga cttcagtatt aaatggagaa
    28801 aagcctcgct attaaaattt atgcctgctt tgggacttaa tttgttttct tcaactgggc
    28861 ttgacaaggc taactctgtc aaaacctcgc ctaataattc ttgagatgtt tcttcacgaa
    28921 gaaccagttc aagcaaatcc attgtaggga tatgggttaa aaataatttt aggaattctt
    28981 ttttagaacc atccgcattg gctatgcaag tagttaatgt tttcttttct tccaccgaca
    29041 attttaggaa aagtttttca agataaggga tttgtttatg caaactaata caagaagaaa
    29101 taattttgtt gtcagttaga agattcattt cttccaaaga tatcagcctt ttctgcagac
    29161 cagaattaga aaaaacatac ctcccgtcct cgttggaaag tgtcaataat ttttttgtca
    29221 aactggctct atggatactg gtattaaaaa ggcaaaatcg gcagtttcgt ttgttgagat
    29281 cgtctaattt gtaagtttct gttttagaat tatcgtgcgg attgattaaa acaaaatcat
    29341 agtttccaga acctttagga ataattttgt ctattctcaa agcatgacgg ccatgaaatt
    29401 ttccaaagct atcgactttt ccataagaca tactaatata aacaggctca tcaggattca
    29461 ttaatttcaa tttgatgata tcatcaagct ttgaatatgg aatgtcttcc gcttgaatac
    29521 caaagaattt gcctaaaaaa gcagtggaag taaaaccggc aattctgtcc ggtatatcat
    29581 gagcaacaac acttgccaaa ggatcagtat tactccaatc tcccgcataa taataggaaa
    29641 ccaatcgttc caaaattttt attgccagtg aattactttt tacccccccg tattgattat
    29701 caatttcctt tagtctttcc gggctaatct caaacacatc ttcattgttg agttcatcca
    29761 cgtaatgggt gtacttccca gtcattttat tcttttgcaa attatctttt agtgcctcat
    29821 ggcgtttgat tcttacgata actttcccat cttccgtttg ggtaaacaac gatttaatta
    29881 attgctcccc atccgcccca agatttaaaa tgcaatccag agatgacaaa agatagcaac
    29941 tacctccaat accttgtcgg atggtaatag ggccttgagt tgggaacaaa ggtgtcattg
    30001 tggtcctctt aaaatcataa cgtacagaaa gagggcatta taacagcaaa atattggata
    30061 atcattaaaa aaaatggtct gtaatcaatc ggaaaactaa agccctaaaa gctaattcca
    30121 ctcataccaa cggtcaagca cggaaatcaa ctcctcaaca ccatcctttt tcacagaaga
    30181 aaaagcttgc acagaaacca aatgctctgc cagctcataa tactgccgaa ctttaagtac
    30241 ggcatttttg acttcactac gacttaactt atctgctttt gtcagtaaaa tatgcactgg
    30301 gagctctctg tgcaaagccc aattcactaa tatttgatcc aaatctttca atgggtggcg
    30361 tacatccatc aacaaaatta atcctctcaa acattgcctt gcttctagat agtgggctaa
    30421 attcttttgc cattccaatt ttacttccat tgccaccttg gcataaccat aacctggtaa
    30481 atcaacaagg cgcctttgct catcaagaga aaagagattg atcagttgag tgcggcctgg
    30541 tgttttgctg gttcttgcca aatttttatt attagttaga caatttaagg cactggattt
    30601 tccagcgttg gaacggccag caaacgctac ctcatacccg gaatcttcag gtaattgatt
    30661 gacacgggct gcacttttta aaaatactgc tttagaatat aaattgattg gcataagata
    30721 tcattttagt tttgggattt tgacccaggc agtgtaccat taattgacta aattattatg
    30781 agaaatccat gaaaaaatta gcactcgtat taatcctgtg ctcttcatca gtgatttttg
    30841 cccaggaaaa cccgcaagag gctggacaaa ataagtcaac agtctgtacc gcctgtcatg
    30901 gtccgcaagg tattagtacg aatcccgaat ggcccaatct tgccggccag catgaaaaat
    30961 attttgtgaa acaattgaaa gatataaaag aaggtaagtc gcgctctgca cccactatga
    31021 ctgcaatagt tgctaatctg aatgagcagg atatagatga cttggcagcc tattatgcta
    31081 aaatgcccgt tgctgaaggt tcaacccctg aaaaatattt aaaaagaggc gaacaacttt
    31141 acagaggtgg tgatttaaac aaacacatcg cggcctgtat agcctgccat ggtcctaaag
    31201 gaagtggaaa cgctcaagca ggctttcccc tgctttctgg tcagcatgca gcttatacta
    31261 taatgcaact gcaagcattt aaagacggga agcgaacgaa tgacttaaat caaattatgc
    31321 aagatatcag tagtaaaatg agcccagaag atatggaagc cgttgcttat tatattcaag
    31381 ggctatattg aggaattgac tatgtttaaa aaattaattg ccctattatt tcttatgcca
    31441 atgactgctt tggcaactca atttattgaa ggcaaagatt atcaaaccgt tgccagcgcg
    31501 caattgtcta ccaataaaga taaaacaccc ctgattaccg aattttttag ctatggctgc
    31561 ccatggtgtt ataaaattga cgccccacta aatgattggg caaccagaat gggtaaaagt
    31621 gctcatcttg agcgcattcc agttgttttt aaaccaaact gggatttgta tgccaaagcc
    31681 tattacacag caaaaaccct ggctatgtcc gataaaatga atgccatatt gttcaaagca
    31741 attcaggaag ataaaaatcc actggcaacg aaacaatcga tggttgattt ttttgttgct
    31801 catggcgtag acagagaaat tgccaagagc gcttttgaaa attcgccaac aatcgatatg
    31861 cgcgttaaca gcggcatgtc tttgatggct cattatcaaa ttaacgccgt accagccttt
    31921 gtcgtcaata acaagtacaa aactgactta caaatggcag ggagcgaaga gcgtttattt
    31981 gaaattttga attacctggt aagaaaatca gcctaattaa ctcaagctat aaaggatatt
    32041 cctaatcagg tcagtaccct catgtttcaa tattctctaa aagccagatt tggttgggat
    32101 gggggctgat ctcttacgta tattcctcgc caaggtaatt gaccacaggc caggtcattt
    32161 tgacctggct ttcttgctaa gcccccttgc ctgggttttc cagactcaaa ataccggtat
    32221 tgaaatcata agcaaaaatc tcggcatatc gtccccagtc aatcattgtt ttcaaaacac
    32281 gatctgcttc tttctcgctt aaataatcct ccagcttact caaaaagcgc tcttccgaga
    32341 cgcgatgccc cgctttctca tccagtacac gtcttatata acgagccaga gggacttttt
    32401 ccaataatct ttgcgcgaac aattgcttac gctcttgcaa atcagcttct gaaaattgct
    32461 ttcctaattc acttaactga atatctcccg cagacacctt ggcaaaacca agtatctcca
    32521 gagtttcaag aattgggaac agatcatcaa tattcatcat gagctcgtct gccagttcag
    32581 gtaaatcaat acgttcttca aaggacttca tagtttctat cagaccggat aattcagagg
    32641 gctcgacatc gggtaagcga tagcctaaac ctatttgacg ctccctttgc gctcgcttgg
    32701 ctttttcttt gggtcctgta gtcattaaag tataaatctt atcaaccaag gcaagatatt
    32761 ccgggctttc cgggtcacgg ggttggggaa gagtcactgg taattcagca cgaatatagc
    32821 cgggatcatt accaaaaatc actatcctgt cagccaaagt cgccgcttct tctatattgt
    32881 gtgttactaa taaaatacca ttggtattcg ttttcttttc tttccataac tccaataaat
    32941 ctgatttgag gttttctgca gtaagtacat caagcgcgga aaagggttca tccattaaca
    33001 acacatccgg attaataacc aaagcgcgag caaaaccaac ccgttgtcgc atcccaccag
    33061 ataactcctt gggaaaagcc gattcgaaac catccaaacc tataatatct atcgcttcaa
    33121 tagccctgtg ccttctttcc tcgcggctga ttccttgtgc ttccaaacca agctccacat
    33181 tttccaggac tgtaagccag ggcattaaag caaacgactg aaataccata gcaatgcctt
    33241 ctaccggcct ggttacaggt ttaccgcggt aagtcactgt tccactggaa ggagcgataa
    33301 gaccagcaat aatcctgagt aaagtggatt tccctgaacc agatttacct aacaaagcaa
    33361 ctatttcgcc ttcctgtaat ttgaaattaa catcttcaag tacaagaagg tgctgggaag
    33421 gtgctttttt aaaagatttg cttaaatttt ctatgttaat aattgtttcc ggcataggag
    33481 tctcaattaa aattaaatcg ttcttcggct aatcgataaa gcgggcgcca aataagatgg
    33541 ttgaaagcta atacatagac gcacatcatt gctgttccta acgcaatttg cgggaagtct
    33601 cccgctgtcg tactagcctg gatatactcc cccagacctg tggccttaag agtaatattt
    33661 ccccagctca cgtattcagc gacgatactg gcattccatg caccgcctgc tgctgtaata
    33721 gctccggtaa tgtaaaaagg aaaaatacct ggcaaagcta atcttttcca ccatatccaa
    33781 ccctttaagc caaaattatc agctgccaga taaagatcgc ggggtatact cgaggctcct
    33841 gcgatcacat taaataaaat ataccactgt gtgcccagga tcatgagcgg agttacccag
    33901 atttccacgc tcaaattaaa tttaacaata gcaattacaa ataaaggata aaacaggttc
    33961 gctggaaacg ctgccacaaa ttgaataatc ggttgtattt tttgagccag ccttggcctt
    34021 aatcctatcc acactccaac gggaatccat agcatagagc ttagcaaaat taatataatg
    34081 acccgagctc ctgttgctgc gcccaataaa aagacatgaa atatatcact caccttcaat
    34141 tcagcgagaa taaatttcaa caaaaaccac ccgccagtaa taacggctat cagaaccaac
    34201 gctgtccaca atcggtccaa acgcttttgc cttttaaaat caatttcctt aacagccttc
    34261 acctcaccaa tcctcagcca acgagcattt acaaaactat tggtaaagac cgcgagccac
    34321 tccgtcactc tcttcatcaa acgactactg cgtatcaagt caattaacca agattgatat
    34381 tctgactcat cgggagactg ttccatttta aatttttcag accaggcaat aagtggcctg
    34441 aaaagtattt gatcatacaa aaaaataacg ataaccatgg tcagaatggc atatcccaca
    34501 gcatgcaaat ccctctgggc tatcgccaat gcgatataag aacctacacc gggtaatcta
    34561 atattttggt gcgccaccga aatagcttct gacaaaacca caaaaaacca gcttgccgac
    34621 attgaaacca tcatattcca taacagtcca gacatggaaa aaggaacttc taccttccaa
    34681 aagcgctgcc aggctgacaa cctgaacata gcagagactt caatcaaatc atgaggaact
    34741 gtttttaaag attgataaaa tccaaaagtc atattccaca cttgggcgct aaaaatagca
    34801 aaaattgagg cgcattctgg acctaacaag ctattgggaa ataaatgaat aaacccggtg
    34861 acagtaatag ataaaaaact cagaacagga atcgattgta aaatatcaat cgccggtata
    34921 acaatttgct cagccctgcg atttttggca gctaaagcac ctacaataaa agtaaacaga
    34981 attgaaaaaa tcaatgcgat aaacatgcgt aaaactgttc gtaacgcata aaagggaagg
    35041 ttagaaggct ccaaagaaat gggaatttga tcccctaatt gatacggggt agccatctgt
    35101 gaaccggccc aacccaggaa aaataacaca gaaaaagtca atataagcaa tagcaaatcc
    35161 catcgattaa taaatcgact gaatttatca ggattagcaa aataaaaccg actattgccc
    35221 actataccct ctcttaaaat tgacgttaca agtttccctt aatctaatcc ttaaatctaa
    35281 aaaatatttg tccgttcctg aatcaggatt tacgaaaaac cccaaggtca aggcaaaaaa
    35341 gtttttaatg agggagttta gataaactaa acgaccgaat taaaaacttt tttaacaaag
    35401 a
//
LOCUS       DAXLDT010000014        35239 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXLDT010000014 DAXLDT010000000
VERSION     DAXLDT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35239
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.014"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            173..439
                     /locus_tag="ACF2SP_000568"
     CDS             173..439
                     /locus_tag="ACF2SP_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358374.1"
                     /translation="MESMIDLVGYVASSLVFATFYVKKILTLRLIAVCSNVAFITYGL
                     GYHLHPIFILHSVLLPLNLYRIFELLKNDPEFTILARINLKATR"
     gene            493..912
                     /locus_tag="ACF2SP_000569"
     CDS             493..912
                     /locus_tag="ACF2SP_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HFD2358375.1"
                     /translation="MCNWNRIVGLALMGFSAFLQAKTYIPQCPKEINTLERIQTSPDG
                     WETLSGIKNNYLGNVSFYSGHPKGQASLKPNRINKKKAEWKFSPHDTIYIVCHYNQTG
                     IELTQQLPQKTKGCTVIFNPNAKGPYGFLPQKITCNQ"
     gene            complement(922..2202)
                     /locus_tag="ACF2SP_000570"
     CDS             complement(922..2202)
                     /locus_tag="ACF2SP_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358376.1"
                     /translation="MNTLSFKHPLLAKTYLWIIWTIAACFFFYKYLIQVSPSVMTADL
                     MKAFQVNAAGLGNLSAFYFYSYLFMQIPVGLMLDRYSPRLLTTLAIIVCSISTYIFSQ
                     TNTLWLACLSRALMGAGAAFAAVSCFKLAAVWFSPKRFALVSGMFMTAAMLGAVGGQM
                     PLAFLVQHEGWRKALELVSVMGIVLGVVYFLVLRDKPAQTQLTQNSKEPVIQLLRRII
                     VNKQAWALSLYSGLAFAPVSVFGGLWGVPFLEKAYLLSRTDAALAISFIFVGFAAGAP
                     FWGWFSDFIRRRKPVLFTGTCSALLCLLVVIYSSNQNLLTLIILLFLFGFGASGFFTS
                     FAMIRELFPLVLVATVLGIMNTFNSVFEALFEPLVGALLDWTWEGTVVDGIHQFTLHG
                     YYFSLLLLPLSLILALLTLLLIDETYCRTKEEAE"
     gene            2679..3959
                     /locus_tag="ACF2SP_000571"
     CDS             2679..3959
                     /locus_tag="ACF2SP_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFD2358377.1"
                     /translation="MELTTTENQLFKSSKVSLALVTVLVGIGSGFLGMCLAMLLHYLQ
                     HIAYGYSPLHIISNETFLEGVRASSSERRIGILFLCGLIAGMGWWALYRFGKPLVSIA
                     SAIKSGKPMPALSTFIHAFLQIITIALGSPLGREVAPREVSSVFAYWLSSKAGLTPEE
                     SKIMLACGAGAGLAAVYNVPLGGAVFVLEVLLCTSNWTVLLPALSSSAIAVLVSWWGL
                     GNESIYQLPEFSLSTPLIIWSILASPVLGFFAFWFIQIATIQRSKAMHNWQMILACLI
                     NFLIIGLFAIYFPSLLGNGKSPAEMEFDQSVGIGLSIILFMLRNVFVWSSLRVGAQGG
                     LLTPSLANGALLGAILGGIWNLYLPPAPIHAFALIGATAFLAAAQKMPLTAIILIFEF
                     TRINFIFLIPIMLAVSGSIAMCQLTKNYYIKYKV"
     gene            complement(4158..4373)
                     /locus_tag="ACF2SP_000572"
     CDS             complement(4158..4373)
                     /locus_tag="ACF2SP_000572"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2358378.1"
                     /translation="MTIDLLVETILPDLKKTKGGCWWLGRKLRACIHLGVYLLQQLFN
                     KTDRQIEYEVKDNAAYQLFCGVGIVEQ"
     gene            complement(4542..5609)
                     /locus_tag="ACF2SP_000573"
     CDS             complement(4542..5609)
                     /locus_tag="ACF2SP_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358379.1"
                     /translation="MDYVDDFISIEEHSSRDDVTDELSLAEQVFTRLQIYSEGFKSSF
                     FINKDLANLGLTSKTLNGVVCSELNQRAAQKLLTHVVKGEQDKAQAMIEKNPGLLLIK
                     SKAVDYSGRTIVGTAFQAAIGAGDKPMWEMMLPYFERLEQGEALRQFDEQFPYGIEET
                     PAAELKAYYNALASAIIHDEDHGLSVIEGFRKEITCQKEITQGKHFNLQHLIAAYQAY
                     IDNFYAFGNWDNRDLFWQKVIGYVQRQMTAYDAQIHCSGVRSVLDKESSFSRTFELSN
                     DEKLFPLQIDSGLGFDFACYSYYSARPLDSGSHNVLPSPLNEYWIWIEKLCGEKRIHL
                     LNLESHLSKGCIISSNNCSLL"
     gene            5850..6353
                     /locus_tag="ACF2SP_000574"
     CDS             5850..6353
                     /locus_tag="ACF2SP_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358380.1"
                     /translation="MPKKFTRKIPLISREECFALLPGYSINAMNLLKRGNHTPYYEEI
                     ESLSKIFVALVTHYSEPIESIINCMEKAMYDAYKEELDKIEHEIQRLFKIRKNTPIHS
                     LFGEREIEKLTIQISELEKCKSVSVKEIIQGVIYDTLDKASQTLVDRIPGNYKLQEYE
                     SRGNPFQ"
     gene            6617..7096
                     /locus_tag="ACF2SP_000575"
     CDS             6617..7096
                     /locus_tag="ACF2SP_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFD2358381.1"
                     /translation="MTLCPCGSQNKYEQCCGIYLESKKTPETPEQLMRSRYTAYSLAK
                     IDYIKQTMQGKPLIAFNESEARQWAQNVSWLGLKIIQSYMERPDKGFVEFIATFLDGN
                     QLKNIHELSEFQKDNSVWFYVDGEAKQLPNKQDKQKIGRNSPCPCGSGKKFKNCHDK"
     gene            7116..8921
                     /locus_tag="ACF2SP_000576"
     CDS             7116..8921
                     /locus_tag="ACF2SP_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HFD2358382.1"
                     /translation="MNGHLLLNVFIFLAAASIMVPLASRFRLGSVIGYLTVGMLIGPF
                     GLKLIGNAEQIMHFAEFGVVMMLFLIGLELEPIMLWKLRKLIVGLGGLQVLLTTIALS
                     SAAVMMGYNLRASVAVSMALALSSTALVLQMLQEKNLLKTAEGETSFAVLLFQDIAVI
                     PILVILPLLGNHHGINVAINPHTAASIAQLPKWLNAIVVAGIIGSIVLAGHYLSRYLF
                     LIIAKTNIREVFTAFSLSLVVGITLLMETIGVSPALGAFIAGMVLANSQYKRTVEADI
                     QPFKGLLLGLFFISVGMGINFNLLFSQMAVIIPIVLGLITIKALILFTLGKIFQLTKI
                     QALGFALGLSQGGEFAFVLFQYSSTLKVISPEINSFFTLVVALSMLATPFLMLLYHRF
                     MMPKLISKLPEREYDSIHEKNNIIIAGYGRFGQIIGRFLSGENIKVTVLEKNPEQVEL
                     LRKFGYTGYFGDANRLDLLKSAGATHAQLLIVTVGNPDTNLEIVKLAKEEFPQLKIFA
                     RARNRRHAYELHKAGVHYFKRELFDSSLTMTKEILKFLGYKPEEIEKKANAFQKHDEE
                     TLIKSFDFFEEEASLINFSRQAKGELERILQNNNP"
     gene            complement(9097..9804)
                     /locus_tag="ACF2SP_000577"
     CDS             complement(9097..9804)
                     /locus_tag="ACF2SP_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358383.1"
                     /translation="MIKAKAKDYSGRTILGTAFQAAIGAGDKPMWEMMLPYFAGLEKG
                     EALRQFHEQFPEGIEDEVSAEDLKEYYNAIALAIISHKDQGLSAIEKFRKQIAFQGEI
                     TQGKHFNLQHLIAAYQTYIDYFDALANWDNRDLFWQKVIGYVQRQMTAYDAQVHCSGI
                     QSVLETERAFSRTLKFPGGEKFFPLSVDSGLGFDFACFSYRWMPLVIQILPPTPRSRC
                     ERCQAKAKDRSGPLKII"
     gene            complement(9801..10103)
                     /locus_tag="ACF2SP_000578"
     CDS             complement(9801..10103)
                     /locus_tag="ACF2SP_000578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358384.1"
                     /translation="MYSKGDKELRNDSGMDYNNYVTNEPSIEEQVFDAMHICSEFKPG
                     FFTTRDLYNLSLASKMLNSLASSEMIQRAAQKLLTLVVLGEQDKARAMIEKNQSCS"
     gene            10498..10833
                     /locus_tag="ACF2SP_000579"
     CDS             10498..10833
                     /locus_tag="ACF2SP_000579"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HFD2358385.1"
                     /translation="MDKSTLRQRARNLRKNSTDAERHLWYYLRAGRLGFKFKRQVPMS
                     AYIVDFVCLEKRLVIKLDGGQHMDNKMYDTKRTDWLMTNGFKVLRFWNHDVFQQTPSV
                     REVIMSALL"
     gene            complement(10857..11177)
                     /locus_tag="ACF2SP_000580"
     CDS             complement(10857..11177)
                     /locus_tag="ACF2SP_000580"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2358386.1"
                     /translation="MMMSFAGEGWGEGMSRMVIDDEMWSRLEKLLPKPKGRRGEDDRL
                     FMDAICWMLRTGAAWRDLPPDYGNWKSVYKKGILLSAFFRRLSVLEGLLQGMTKQPGC
                     TSLV"
     gene            11377..12279
                     /locus_tag="ACF2SP_000581"
     CDS             11377..12279
                     /locus_tag="ACF2SP_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD2358387.1"
                     /translation="MIKQLQQALSDVLLGKEEVIRLGITCLLANGHLLLEDNPGMGKT
                     TFSHALAQLTGMQYRRIQFTSDLLPSDLLGVSIYDHQRNVFQFHKGPIFSQLILADEI
                     NRATPKTQSALLEAMEERQVTTDIGTEALPQPFFVIATQNPSCHTGTYPLPESQLDRF
                     LMRLVIGYPPPDAERMLLKSSNLNAQLKELSPIINPQQLQVLQRESQKVLVSELVLDY
                     VQSLLDASRNQGWFNHGLSPRAGMGLVHAAQAFARTDDRDFVRPDDIQAVMPAVVNHR
                     LIIKKSQTQLSAAELLMSEIEVPV"
     gene            12276..13226
                     /locus_tag="ACF2SP_000582"
     CDS             12276..13226
                     /locus_tag="ACF2SP_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358388.1"
                     /translation="MIRQIKTKVMAYWEEWIAKRISKTNPQILGSQNIYIIPSGFGWA
                     YGVVVLSLLSGAINYQVSTVFLMSFILAIVGFISAWEAHANLKGLSIKLVSVEDTYEG
                     TPAQVSLLIQSNNRMRFGLDFRLNNQSVTRLEKIPPQDLRFILPLATTRRGCYTLPKI
                     TISSFFPFGLFQVWGYANFDSNYYVYPQPVNPGFWPIPLYHQNKRKTDSHGDNEYYDL
                     KQVDNPWIQPNLIAWKIAAKGQGWYLKTMDRAEEMYWLFRLNDLPIETLEEKLKHLSY
                     WLVTAESQGQFYKLELGNDPDKFSHGEEHLRYCLRQLAVY"
     gene            13226..15214
                     /locus_tag="ACF2SP_000583"
     CDS             13226..15214
                     /locus_tag="ACF2SP_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HFD2358389.1"
                     /translation="MDFFPKQHIFLITTRYALLVMLICYFPHFLTCPLWLTVFILTTL
                     CYKLISDYFAYPLLDSKIRFIVVIICLFLLKIQYGSIISSGFFIGFLLAFIGLKTIEI
                     HNDRDIRILILCNFYLIFASLIVVQELWIIPYLLIAILANFTLMLKLTAPQASLRQIS
                     GRSMRLLLIATPLTVFLFYFFPRIANPLWQVPSLGKSHTGFSEWMEPGSVANLLNDDR
                     TALRIIFKRKAVLNGYWRGLTLSRYNGISWNPAWHDSSTFSSLKELTTSDDGDYEVIM
                     EPHQKKWLFYLGYPNAAYPQLLFSPNFGLVSHNKDMIAQRFAYALRISSSPHSSLTQK
                     DWKLNTQLPKYSNPRITFWAKKQFAEVNYDPQAFIQFIQNYIKRESFWYTLTPPKLDN
                     ENDQMDYFWFDSRKGFCEHYASAVTVVLRAVGIPARVIVGYQGGEWNPLAKYLTIKQY
                     DAHAWVEYWQKDFGWKQLDPTAFISTSRIDPSVLAMRAARTNQQLIIDPSNLNWIQKT
                     KLYMESTRFFIERWLLFYNQDAQYELLEQIGLKHWNASTLLQIAILSLILFIILLGAC
                     YQWRQKRTLDPLLREYHLLQKEFCRFDIATQPPATLNKQCKILINKIPNLEETITTFL
                     SHYEKLRLRHRITKTKENKKQTLLLFKNLRRILKQVKI"
     gene            15296..16483
                     /locus_tag="ACF2SP_000584"
     CDS             15296..16483
                     /locus_tag="ACF2SP_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFD2358390.1"
                     /translation="MVHFQKMTVQTFLKDYWQKKPLIIRQALPDFTNPLTPDELAGLA
                     LEEEIESRLVYETPDHSPQWNLKRGPFKESDLIGLPKTHWTLLVQGVDRIVPDVYELL
                     EHFNFIPQWRVDDVMISYATLHGSVGPHYDNYDVFLYQAKGRRLWSLTSKKCHTNNFI
                     KGLELRIMKEFEVEEQFILEEGDMLYLPPHIGHYGIAQSEECMTYSFGYRSYQGQELW
                     DSLGDYLSEHGLFKSLYQDPDWSTLKSTSEITPKAWDNARQLLRQVLENDQLMQSWFG
                     CFATSLDQSAEQNLPPPLEEDELLGLDEFIKELANYQEIVRDASCRFAYIMSDQESQC
                     HFYVNGKEWDSRGVSTNLLSLVANNRFLSLKELKPYLSHKTNQFFLYELWKLQWLQIF
                     HDQ"
     gene            16591..16917
                     /locus_tag="ACF2SP_000585"
     CDS             16591..16917
                     /locus_tag="ACF2SP_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358391.1"
                     /translation="MARFSRISLSTVLLSLICFNIFASDTIMITVNTKEKNAIAIGYS
                     VNGNQYGGLGSSYSGHGPKNKEYQFGYKKDSIFGKDISCGSMTLNQDSKVTLVNNNNQ
                     CISVLG"
     gene            17172..17321
                     /locus_tag="ACF2SP_000586"
     CDS             17172..17321
                     /locus_tag="ACF2SP_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358392.1"
                     /translation="MKKIILAVVVISAAVLLSGCGGGCCGASNSCCSYNTGCCGVQNY
                     SGIWY"
     gene            17353..18255
                     /locus_tag="ACF2SP_000587"
     CDS             17353..18255
                     /locus_tag="ACF2SP_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2358393.1"
                     /translation="MKKNSLLAISEEGFHHIAYTEWGIFEPELPTVICVHGYTRNSRD
                     FDELANYLSSKGRHVFCPDIAGRGDSSWFKNPRHYNFTQYVKDITALIARANAHQIDW
                     IGTSMGGIIGMILAAMPNSPISKLVLNDIGPQIPIHGLRRIAKYAGKDPDFKSLEEAK
                     QHFKASYADFGITNEKQWDVFTKNSVEQRGPNLYVTKMDPAIKRSKSILQIISEFFRH
                     PHKALEGIFYDINLWSIWKQIKCPVLVIHGVHSDILTSEIITQMKKTHALTEVYEVEN
                     TGHAPALLNLADHKKIEDWLTTHQ"
     gene            complement(18421..18663)
                     /locus_tag="ACF2SP_000588"
     CDS             complement(18421..18663)
                     /locus_tag="ACF2SP_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358394.1"
                     /translation="MIQLRWSGLAASKILFGLLITLLPESWPKIIAVDETLERCRGKK
                     IKAKGASRGVKDHGLRLLWLSVHHQKSQMKLQSFAA"
     gene            complement(18890..19819)
                     /locus_tag="ACF2SP_000589"
     CDS             complement(18890..19819)
                     /locus_tag="ACF2SP_000589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358395.1"
                     /translation="MPKSIKDLYPDTAKEISGYLSTIKDKTNSIVDCKTRTLFQPPAI
                     LSKFLEFVAHGKQDHAEKIFTIRGKNPEQLQELLLKQGTVTDYSGRTFTNISAYEYAY
                     WAKDKHMCRMLESHMDENTKAAMLKRCEAIEKNGLTFKQHGVEVKNSKHFDFTPLKTA
                     LKNFIDGYDSWEMTQNWTAIKEAWMNVGKAQRDVPVHVANEYCRKDRSFDPTPQFNED
                     KLPRELTFYNFNTGKVGAWFPLAISDSSGLGVDFALNCGVAPGGPLMAKLGAVDALAA
                     IIHLDEVRTADLTVSLKNLSLAKPGLDDDYCFF"
     gene            complement(20094..21008)
                     /locus_tag="ACF2SP_000590"
     CDS             complement(20094..21008)
                     /locus_tag="ACF2SP_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFD2358396.1"
                     /translation="MNSQEQRGSFYAILSGFLYGFIGYFGISAIHGDLSASNMLFWRF
                     LISSIFILIVLIPQLKNINDTYKSMFTAFLSGVLYYGLSTLLYFYASLYIGSGLSMVI
                     FFTYPVIIMVFNYFFYSQTIPKIYYLAISVILIGMALLIDVNEIAFDLWGIFLGLASA
                     FFYACYIVSSKNNEISPNVSTLMVCLGCMTTSLIVACLDQTFKVPTSFPVWLNLFGIG
                     LVATVIPILLMLHSLKYISSEKASILSVLEPVFVVIFGVVLLGEELKPWHALGIVLVL
                     AGALITLFSHRINLNQLKQQLFQFLKLT"
     gene            21266..22405
                     /locus_tag="ACF2SP_000591"
     CDS             21266..22405
                     /locus_tag="ACF2SP_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HFD2358397.1"
                     /translation="MFRVLIFLSCLVILTPNAIADTNPCEKHSCIAVIDAGSTGSRLH
                     IYSYDTDDTNTPIHIEEIWNKKIKPGFASIQPNSVTIDAYLTMLLADAPIHNIPVYFY
                     ATAGMRLLPQSQQKKYYDELEYWFRQQSQWQLVEAKTITGNDEALFDWLAVNYKLDTL
                     KSVQNKSVGVMDMGGASVQIVFPMPKNVEISKHNQVELNIYGQNINLYVHSFLGLGQT
                     EMSHQFLNSPSCFANDYPLPDGESGQGNAPSCKEEVTSLMNSVHKVNQQIQPLLALNP
                     VNEWYSIGGISNLASSQLFHFENSELTNQSLLQQGDNQICHQQWDILNGQYPDDEYLY
                     QYCLLSSYYYALMVDGYGINPNQTIHYIPPEQNLDWTIGVVLHRA"
     gene            complement(22410..22943)
                     /locus_tag="ACF2SP_000592"
     CDS             complement(22410..22943)
                     /locus_tag="ACF2SP_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFD2358398.1"
                     /translation="MHHVEDLISIFNYCFENDYNTRLVRGGDEPIYLPADDQRPYHAL
                     FFAHGFFSSALHECAHWLIAGEERRKLVDFGYWYAPDGRNEEQQTLFQRVEVKPQALE
                     WILSMAAGHKFRISIDNLNGAEVDTEAFKQSIYKQVFHYCTQGLPQRALHFRNALCAF
                     YRQPSPLRIDDFSIDEL"
     gene            complement(22954..24759)
                     /locus_tag="ACF2SP_000593"
     CDS             complement(22954..24759)
                     /locus_tag="ACF2SP_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1907 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358399.1"
                     /translation="MVRECTSFKGGLNLNFIENAEFESRKNIPPEEQRAVLIRNTLRL
                     LMMGWNTSWTELFSWETFKAIYLQRDPLLLKEFRLAFQKGFDALFTQLSGRQFNSQQN
                     EQIQLYLSNCLSYLPLFDLNPYESIKVPQRINDQWVMVDYYIKPIELTNHRGKNDLIL
                     RDDDRVFAYGLEPIDNPNAQPHLIFMGTTYPAGQGFISQLNTDLKGFETVGYSLYKNG
                     RDRIKDWILKQNSKIHVCGISLGGSLSLLLAIDMGKYLSRVDALNPAGLHDTDNKVKY
                     DKWDKIKKKPLVVVQQQGNDPVSFFGVWKKEWNILHVKPPKDKQGPNLFCDHFLNYAG
                     FAETEFTYVSAEQQNAKRKARNFWLYSLGRGVLYYFGLAPYTYLIRPVIHYIAEKWPL
                     IVFGIIPLLTIGTLIGLSTAGIIPAIVSIGTLAAFAISSGIFYLSFGLSSNEEDKNTW
                     AGMFDFILKGNEKVPDENEIKPAKLHHPSTPRNPEMDIYKNETAIDLTYKQINTYYQV
                     TRCLLKQKEFLPLKDKPEKRLGGLSKKEALLASMNPASHDEVISLQIPKAKAVHIKHS
                     LTLAAKIGMENEIELKSALKKEYNQYQIGKHQRFC"
     gene            complement(25086..25691)
                     /gene="gstA"
                     /locus_tag="ACF2SP_000594"
     CDS             complement(25086..25691)
                     /gene="gstA"
                     /locus_tag="ACF2SP_000594"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFD2358400.1"
                     /translation="MKLYYCRGACSLVVRIILNELGLNFQDEEVDLTVKKTAGGDDFF
                     KINPKGAVPTLVLDNGDVLTENQVILQYLADTTPGQKLLAAVGESKRYHTLEWLNYIS
                     TELHKSLGMFFNPTVSEEIKSKILTPLVMTRFAYTNTRLAKGSYLMGNEFSLPDAYLF
                     VMVSWAKYFKLDLSQFEHLTKFMDLMLARPSVAKSLQQEKL"
     gene            complement(25888..26883)
                     /locus_tag="ACF2SP_000595"
     CDS             complement(25888..26883)
                     /locus_tag="ACF2SP_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358401.1"
                     /translation="MAICINKETDHFFISIGKINQHSFIMLGVYDDFQVSHLLCRVGK
                     IFDLPNQTKGTKRCLSIYSALGGAIFASSKAKIEDEGITRKRKGSVPISYQAYDISYE
                     QYCEFVHYLESIQTESNQFECFKPLVQNGNAVYFSQTSSRVFATGSHWKELNEEIHQI
                     NTGNTCRHSAIKLIEAVTKTSVPSSISSCFFINLPYKTQLDYGKPSQNIPFYVLPPPP
                     PSIHPGFNKEKCLIAKKLYHRIEQLPVLEPNSPMTKRKFNSLKNLYLQIIGSQKNQSI
                     DELLFGIQQWKEKNRVDLQTLRRTYFWDSFIFRESATMKLINEIEKDLKCAKCPY"
     gene            complement(26924..28186)
                     /locus_tag="ACF2SP_000596"
     CDS             complement(26924..28186)
                     /locus_tag="ACF2SP_000596"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFD2358402.1"
                     /translation="MKINNLVSFLLAFCLASLSYNISAAESPVVSHHKIQKEMSKYFK
                     VYHNKEKFTAIAVSVFIPQEKNNDALDVATFVKGTMGYPPLSQEITPLNLFDIGSITK
                     SFTSLILLQLQTEGKLSLDDPLGKWLPQYKEWKDVKLRQLLNMTSGIPNYSEDPEFAK
                     KMEDNFAAIWGDEELLGYAHPEKPIETDRANRYEYSNSNYILAGMVIEKVTQDTFENQ
                     LKQRIINTNNGLNNSFYPAGPHGPEVEKSIRDRRVHGYFYDEDNKKLIDTISNDLSWA
                     GAAGAVIANTEDVGRWVQLLYHGLLIEPAFRDEALSELESVVSIKTGLPIPSVTEDEP
                     YGFGLGVGFYYDKNSRQRFWTYQGSTLGYRVMYLWNPCNNVTVVVALNGKGGSGNEKS
                     KMGNHIHELCLNIHKVIVKNHPHLQCAS"
     gene            complement(28433..29845)
                     /gene="gltX"
                     /locus_tag="ACF2SP_000597"
     CDS             complement(28433..29845)
                     /gene="gltX"
                     /locus_tag="ACF2SP_000597"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFD2358403.1"
                     /translation="MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDT
                     DRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLKEGKAYRCQCSK
                     ERLEALREAQLAAKEKPRYDGHCRNQILPDSGVPYVIRFRNPDAGIVSFHDEVYGDIH
                     VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDA
                     PVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
                     SVQEMINSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSQESVANALQWHFEQAGIDLN
                     QGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHE
                     RLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKN
                     KSIKRLEDALEKIRARASAV"
     gene            29944..32676
                     /gene="letS"
                     /locus_tag="ACF2SP_000598"
     CDS             29944..32676
                     /gene="letS"
                     /locus_tag="ACF2SP_000598"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LetS"
                     /protein_id="HFD2358404.1"
                     /translation="MLKSIGIKYQLRITTLIPAFLVALLFAFFYNGLFGKDLKQHMSR
                     LGEAYIRQLLPAAQYAMLRNDYRTLQGLINASTINPEVKALAFYNADGRLIAYRGGKH
                     SIHKPFNPPDYTGDYIESKQINPFTINFIAPITIPKFNLYSSTEFKELSTPKIFQADD
                     ILGWLSIDIDTQSLLIKRYQMLIVTIFITLFGLLMGLTIHYFLSKRIYMPIARLRRSM
                     KQILSNEFETEIRVSSPGELGIIEKGCAHLQRQYLNTVRDLNHHIEIATADLQQSLEL
                     LEEKNIELSLEKKKTEEKSRQKSEFIANMSHEIRTPMNGVIGFTNVLLESKLDPLQLD
                     YVKTIKSSAQDLLSIINDILDFSKIDAGKLNLDCIPLDIRGCIDEVLSLASPNAHKKG
                     IDLIPITDINVPKMVLGDPLRIKQIISNLVTNAVKFTDHGYVLIRTKIEQETDKDYTL
                     LFAITDTGIGISPEDQTKLFTAFNQADTSITRRYGGSGLGLVICKKLCEEMHGRISLT
                     SEINKGSTFSARIKVEKLVAYEIEKNQTHRFAHLKIICFDDNPLHLEAIGNGLGFWGI
                     EAIRVDSFNKLSRILTKHKDCKIAFINVNQGCERQAAELIAKHKQIPFVLISKWPIND
                     FAALGARGFLYKPISIQKLQDLIESIANENQTEKNTNQELDTLREQLRFLHPEILIAE
                     DNPVNKMLLTSLLNNNANITTVDDGEMAVTACEDKKFDMILLDLHMPKLNGLEAAKMI
                     RQKSLMNKHSPIVLITASSSDLSSIDMKKSGVDFCFQKPIDEKQLLIQILRIVDKTKH
                     AAIDWQLCVQKVSGNQALAEEFLAKFIEELYKNREEFIELMHQKNVKGLADLAHKLHG
                     ACCFCGVPILQKRVAQLEKLARRTANADNLTGAFTDLIQSIDAVISEYENQYSQ"
     gene            32774..34018
                     /locus_tag="ACF2SP_000599"
     CDS             32774..34018
                     /locus_tag="ACF2SP_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFD2358405.1"
                     /translation="MSVKNAIYAQSGGVTAVINASACGVIQTAKLFPEQIGKVYAAKN
                     GIIGALNEELIDTSLESEEDIAKLMQTPSGAFGSCRYKLKDNGEEYQRLIAVFKAHNI
                     GYFFYNGGGDSQDTAHKVSQLGKTMGYPITCIGIPKTVDNDLPFTDNCPGFGSVAKYI
                     AISTKEAGFDVASMAASSTKVFILEVMGRHAGWIAAASGLAEQNPGEPPHIILLPEVP
                     FEPTRFLKKVKDCVNSYGYCVIVVSEGIRNKEGKFLSEAGLRDAFGHAQLGGVAPVIA
                     QLIKSELGFKYHWAVADYLQRAARHIASQADLDQAYALGKAAVEYAVKGYNAIMPIVI
                     REQDEPYQWSIGQVPLAEVANQEKAMPPEYIAEDGMGITDACKRYLSPLIQGEAYPNY
                     KNGLPDYVRLKNQLVAKKINQD"
     gene            complement(34015..34428)
                     /locus_tag="ACF2SP_000600"
     CDS             complement(34015..34428)
                     /locus_tag="ACF2SP_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFD2358406.1"
                     /translation="MRTKGFTLIELMIVAAILGILVAIAMPAYQDYTIRARVVEGLEM
                     ASAAKLAVVETSLIDNTLPANQADTGYTSPAPTINVASIAIGNQGIITITYTANAGNG
                     TITMVPTLDATNRILTWDCTGGTLISKYRPAYCRP"
     gene            complement(34622..35032)
                     /locus_tag="ACF2SP_000601"
     CDS             complement(34622..35032)
                     /locus_tag="ACF2SP_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFD2358407.1"
                     /translation="MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTL
                     ADSAKLAVSETAITNNALPATQAATGYVSPAATPNVASIAIGANGVITITYTAAAGGG
                     TIVMTPTLQANGDVTWTCTGGTLLAKYRPASCRP"
BASE COUNT        11158 a         7054 c         6407 g        10620 t
ORIGIN      
        1 tattgtctcc aaatataaac aaaaaattcc tgtttggtaa tatatttaac tcaaagatca
       61 tcgattatac atttcaacgc ttaagaaaac cataagaatt gattaaaaaa cttgctaatc
      121 tcctctggtc agtgttatgt tgaatgaaag ggatttggaa gtggaggtta aaatggaatc
      181 aatgattgat ttggttggat atgtagcttc ttctttagtc tttgcaacat tttatgtcaa
      241 aaaaattctg acattaagac tcatagccgt ttgtagtaat gttgcgttca ttacttacgg
      301 tttgggttat catttacatc ctatatttat tttgcattcg gttttattgc ctttaaattt
      361 atacagaata tttgaattac taaaaaatga tccagagttc acaatacttg caaggatcaa
      421 tttgaaggca accagataat cattttcatc atttaatgat atgatacatg tttactcaat
      481 aaggggtctt ccgtgtgcaa ttggaacagg atagttggct tggcattaat ggggttttct
      541 gcttttttac aagcaaaaac ctacatccct caatgcccta aagaaattaa tacgcttgaa
      601 cgtatacaga catctccgga tggatgggaa accctgagcg ggattaaaaa caattatctg
      661 ggtaatgttt ccttttattc cggccacccc aaaggtcaag ccagcttaaa accaaaccgt
      721 attaataaga aaaaagccga atggaagttt tctcctcatg ataccattta cattgtgtgt
      781 cattacaatc aaacaggaat tgaactgaca caacaattgc ctcaaaaaac aaaaggatgt
      841 actgtgattt ttaatccaaa cgcaaaaggc ccatatggtt tcctgcctca aaaaataacc
      901 tgcaatcaat aatgaggagg tttactctgc ctcctccttt gttcgacaat atgtttcatc
      961 aatcaataac aaggtcaata aggctaaaat taaagacaga ggcaacaata aaagagaaaa
     1021 atagtacccg tgtaaagtaa attgatgaat accatccacc acggtcccct cccatgtcca
     1081 atccagcaaa gcaccaacca gtggctcaaa caaagcctca aacaccgaat taaacgtatt
     1141 cataatacca agtactgtcg ctacaagtac caaaggaaat aattccctga tcattgcaaa
     1201 gctggtaaaa aatccactcg ctccaaaacc aaagagaaat aaaagaataa ttaaagtaag
     1261 caaattttgg tttgagctat aaataactac caataaacac aatagggcag aacacgttcc
     1321 tgtaaacaaa acaggctttc tccgcctgat aaaatcagaa aaccatcccc aaaatggcgc
     1381 gcctgccgca aaaccgacaa aaataaatga aatcgcaagg gctgcatcag tacgagataa
     1441 gagataggcc ttttccaaaa aaggaactcc ccataatcca ccaaaaaccg aaacaggagc
     1501 aaatgctaat ccgctatata aagacaaagc ccaggcctgc ttattaacga taatacgtct
     1561 taacaattga ataacaggtt ccttgctatt ttgcgtgagc tgcgtttgtg caggcttatc
     1621 tcttaaaacg agaaaataca caacccccag gacaattccc atcacactaa ctaattcaag
     1681 tgccttgcgc caaccttcat gttgaaccaa gaatgccaaa ggcatctgcc ctcccacagc
     1741 gcccagcata gctgccgtca taaacatccc tgatactaga gcaaatcgtt ttggagaaaa
     1801 ccagacagcg gctagcttaa aacaagacac ggcagcaaaa gcagctcccg cccccatcaa
     1861 tgctcgactt aagcaggcca accatagagt attggtttga gaaaaaatat aagtactgat
     1921 actgcagaca ataatagcaa gggttgtcag cagtcttgga ctgtacctgt ctaacataag
     1981 gcccacggga atctgcataa ataaatagga ataaaaataa aaagctgaca aattcccaag
     2041 gccagcagca ttaacctgaa atgccttcat caagtcagct gtcataacac tgggagaaac
     2101 ctgaatcaaa tatttataaa agaaaaaaca tgccgcgatg gtccagataa tccaaagata
     2161 agttttagcc agcaatggat gcttgaatga taatgtgttc acaataactc ctaacttatt
     2221 tgcttcaggt tagagataaa atttcaaaaa ccgccaggtc ttatctgctg ctgttatcgc
     2281 cctgaggcgg ggcagataag tcaggagaga agccgtctca gagcagaaga aagggtaaag
     2341 ctgaccacca ccacgacgac aattgagatg agagagttta aaacaatagg cgacaatcca
     2401 acaggctgtc tgttgcagct ttgggaacaa gactttctgc ttattaattg tcgtcttata
     2461 ttcataaaaa actggaaatt aaaagaatgt ttaatgttaa ctttttaggc aatgtgctgt
     2521 caagatcgca aacaaacaat ggatactttt ttaaattttg ttattacggc tacatttatt
     2581 aatatgtttc acttgcaaaa aaatctgcat acagaaataa taaaattctc acccctataa
     2641 gcaaattgat gacaagttac cttaaaggaa tccagggaat ggagttaacc acaaccgaga
     2701 accaactatt taaaagttcc aaagtaagtc ttgccctggt tactgttctg gtgggaatcg
     2761 gttcaggttt tctaggcatg tgcctggcca tgttattgca ttatcttcaa catattgcct
     2821 atggttacag cccccttcac atcatcagta atgaaacttt tctggaggga gttagagcct
     2881 cttcatctga gcggagaata ggcatactat ttctttgtgg cttaattgca ggcatgggat
     2941 ggtgggcact ttatcgcttt ggtaaaccat tagtcagtat tgcgtcggca ataaaatcgg
     3001 gtaaacccat gcccgctctc agtacattca ttcatgcttt tttacaaatc ataacaattg
     3061 cattgggctc acctctggga cgagaggtgg ctcctcgtga ggtaagctct gtctttgctt
     3121 actggctatc tagcaaagca ggattaactc ctgaggaaag caagatcatg ttggcatgtg
     3181 gagcaggcgc ggggcttgct gcagtttata atgtgccttt aggcggtgct gtttttgttt
     3241 tggaagtgtt gttatgcact tccaactgga ctgtcttgct tccagcatta tcttcttctg
     3301 ctatagcagt tctggtgtct tggtggggtt tgggaaatga atcgatttat caactccctg
     3361 aattttcatt aagtacgcct ttaattattt ggtctattct tgccagcccg gtgttaggct
     3421 tttttgcttt ttggtttatt caaattgcta caatacaacg cagcaaagca atgcataatt
     3481 ggcaaatgat attggcttgt ttaattaatt ttttaatcat tggcttattt gcaatttatt
     3541 ttccttcttt actaggtaac ggcaaaagtc cggcagaaat ggagtttgat caatcagtag
     3601 gaattggttt aagtattata ttatttatgt tgaggaatgt attcgtatgg tcaagcttgc
     3661 gtgtaggagc ccaaggtggc ttactaacac catcactggc taacggcgcc ttattaggag
     3721 ctattcttgg tggaatttgg aatttgtatt taccacccgc tcccatccat gccttcgcgc
     3781 ttattggtgc cacggctttt cttgctgcgg cacaaaaaat gcctttaact gccattatct
     3841 taattttcga attcaccagg attaatttta tctttcttat ccctatcatg cttgctgttt
     3901 ccggctctat agctatgtgc cagttaacca agaactatta cattaaatat aaagtatgat
     3961 tgtttgtaat tagtcgctgg cgcaaaattg attcatgcga tttttctcct gacaggtaat
     4021 ttttgccact taatgagttg ccccatgtta aacccaagca aaactgcttg ctttgccatc
     4081 agattttcca aggtttcttg agtttcttca cttaatcgtg atcgaaactc ttcaattttt
     4141 gtatgatcag ggatatgtca ttgttcaaca atccctacac cacaaaaaag ttggtacgcc
     4201 gcgttatctt tgacttcata ttcaatttgc cgatctgtct tgttaaacag ttgctgtaat
     4261 aaataaacac ccaaatgaat acaagctctt agttttctgc ccaaccacca acatcctcct
     4321 tttgtcttct ttaaatcagg aagaatcgtt tctaccagta agtcaatggt cactcatggg
     4381 tttgctaccc aagcgatcaa aaactatcgt cccggtggtg ttcctggtgg gtaaaaataa
     4441 atgcttaatg gtcttatcag caactattaa tcgagtttat tcacccccca tcaagccgga
     4501 cttaaaattt tggctaaggc tctgtttaat gcgcagttgg gtcacaataa agaacaatta
     4561 tttgagctga taatacaccc cttgctcaag tgactctcta agttcagcaa gtgtatccgt
     4621 ttttctccac ataatttttc aatccatatc cagtattcat tcaaagggga agggagcaca
     4681 ttgtggctac cggaatccaa aggccgggcc gaataataac tataacaagc aaaatcgaaa
     4741 ccgagaccag aatcaatctg taaggggaac aatttctcat cattagacaa ctcaaacgtc
     4801 ctactgaatg aactctcttt atctaatacg ctccttacac ccgaacaatg aatttgggcg
     4861 tcataagcgg tcatctgccg ctgcacataa ccaatcactt tttgccaaaa taagtcacgg
     4921 ttgtcccaat taccgaaagc ataaaagtta tcgatgtagg cctgatacgc tgcgattaag
     4981 tgctgcagat tgaaatgctt gccttgcgta atttcctttt ggcaggttat ttcttttcta
     5041 aatccttcaa ttacggagag accatggtct tcatcatgga taatggctga tgcaagggca
     5101 ttgtaatagg cttttagctc agcggcaggg gtttcttcaa tcccataggg aaactgttca
     5161 tcaaactgcc ttaaggcttc tccttgctct aagcgctcaa aataaggaag catcatttcc
     5221 cacatgggtt tatctccggc accaattgcc gcttgaaatg ctgttcctac aatggttctc
     5281 ccagaatagt ctaccgcttt tgatttaatt aagagcaatc ctggattttt ttctatcatc
     5341 gcttgcgctt tatcttgttc tcccttaact acgtgggtta agagtttctg agctgctcgt
     5401 tgattcaact cggaacatac aacaccattc aatgtcttag aggttaaacc aagattcgct
     5461 aaatccttgt ttataaaaaa gctcgactta aatccttctg agtagatttg caatcgagta
     5521 aatacttgtt ctgctaaaga aagttcatca gtaacatcat ctcttgagct gtgttcttca
     5581 atacttataa aatcgtcgac ataatccatg tccgaatcat tttttactat atgctttttt
     5641 ttgaacacat cgcttcactt tgctagacta ttaaagaggg cagagtttat cacaaaataa
     5701 tcgacatggt cattaattat actttgcaat ggctattgcg cgctagtgac taactatctg
     5761 gaatatgtca aattatatac cacattgatc aaattgaata atatagatgc tataattata
     5821 atgtagacct gatgaaaagg atgttcacta tgcctaagaa atttactcga aaaataccac
     5881 ttatttccag agaagagtgt ttcgctttat tacctgggta cagcatcaat gcaatgaatc
     5941 ttctgaaacg agggaatcac acaccctatt atgaagaaat tgagtcctta tcaaaaattt
     6001 ttgttgccct ggtcactcat tattcagaac caatagaatc aatcatcaat tgcatggaaa
     6061 aagcaatgta tgatgcttat aaagaggaat tagataaaat tgagcacgaa attcaacgcc
     6121 tgtttaagat aagaaaaaat actccaattc attctctatt tggcgaaaga gaaatcgaaa
     6181 aattgactat acagatttca gaattggaaa aatgcaaatc agtgtctgta aaggaaatta
     6241 tccagggagt tatctacgat accttagata aggcaagcca aactctggtg gatagaatac
     6301 cgggaaacta taaactgcaa gaatatgaga gccgaggcaa tccttttcaa taagattcag
     6361 ttcattgtta aatgaaacac aaccaatcac ttataaccta atttactccg atagcacttg
     6421 gtcatttttt tattacccat tttaaaatcg ctgtgtggat taataatgaa tcacactttg
     6481 tccctgtttt tataaaaaaa atccaatgca ataaaactgt ttagaaataa tggaatgcca
     6541 tatagaatat aatcagggat tagttaatat aatggattgt aaatttaccc ataaaccagt
     6601 ttttacaggt aactaaatga ctctttgccc ctgtggttca caaaataaat atgagcaatg
     6661 ttgcgggatt tatctggaaa gcaagaaaac tcctgaaaca ccagaacaat taatgcgttc
     6721 gagatacact gcgtattctc ttgcaaaaat cgattatatt aaacagacca tgcaaggcaa
     6781 accactaatt gctttcaacg aatcagaagc aaggcagtgg gctcaaaatg tttcctggct
     6841 gggtttgaaa atcattcaaa gctacatgga gaggccagat aaaggatttg ttgaatttat
     6901 agcaaccttt cttgatggaa atcaattgaa aaatattcat gaattaagtg agtttcaaaa
     6961 ggataattcc gtttggtttt atgtggatgg ggaagctaaa cagttaccaa acaaacaaga
     7021 taagcaaaag ataggccgca atagcccatg tccctgtgga agcggtaaaa aatttaaaaa
     7081 ctgtcatgat aaatgaatta aactcaaggt agcagatgaa cggtcactta ctcctcaatg
     7141 ttttcatttt ccttgctgct gcaagcatca tggttccact ggcaagccga tttagacttg
     7201 gttctgtgat tggttatctt accgttggga tgctcatcgg accttttgga cttaagctaa
     7261 taggcaatgc cgaacaaatt atgcattttg cagaatttgg agtagtcatg atgttattct
     7321 tgattggttt ggaattagaa ccaatcatgc tctggaagct gagaaaatta atagttggct
     7381 tagggggctt gcaagtgttg ttaacaacaa tcgctctgtc ttcggcagct gttatgatgg
     7441 gatacaattt gcgagccagt gtagcggtca gtatggcact tgccctatct tcaactgctt
     7501 tagtcttgca aatgctccag gagaaaaatc ttttgaaaac cgcggagggt gaaacttctt
     7561 ttgctgttct cttattccag gatattgcgg tcattccaat cttggtgatt ttgcctttat
     7621 tgggcaatca tcatggaatt aatgtggcca ttaaccctca tacggctgcc tctattgctc
     7681 aattacccaa gtggcttaac gctattgtcg ttgccggcat tatcggtagc attgttttgg
     7741 cgggacacta cttatccaga tacttgttcc taatcattgc aaaaaccaat atccgtgaag
     7801 tatttacagc attttcctta tccctggtcg ttggaattac cttgttaatg gaaacaattg
     7861 gggtgtctcc ggctttggga gcttttatcg caggtatggt tttagctaac tctcaatata
     7921 aacggactgt agaagctgat atccaacctt tcaaagggct gcttcttgga ttatttttca
     7981 tctcagtcgg aatgggtatc aacttcaatc tactcttcag tcaaatggct gtaatcatcc
     8041 ctattgtact cggtttgatt accattaagg cattgattct ctttacgctc ggcaagatat
     8101 ttcaactcac taaaatacag gcgctcggat tcgcccttgg tttatcacaa ggtggagaat
     8161 ttgcctttgt tctttttcaa tactccagca cattaaaagt aatcagcccg gaaataaaca
     8221 gttttttcac tctggtagtc gccctgtcca tgttggccac acccttttta atgcttttgt
     8281 accatcgttt tatgatgccg aaattaataa gcaaattacc agaacgtgaa tacgactcta
     8341 ttcatgaaaa aaataacatt attatcgctg gttacggtcg atttggtcag ataattggtc
     8401 gatttcttag tggtgaaaac atcaaggtaa ccgttttgga aaaaaatccg gagcaagtcg
     8461 aattattaag aaaatttggc tacaccggat attttggtga tgcgaatcgt ctggacctgc
     8521 taaaaagcgc aggagcaacg catgctcagt tgctcatagt cacagtcggg aatcccgaca
     8581 ccaatttgga aattgtgaaa ttagctaaag aagagtttcc tcaattgaaa atttttgccc
     8641 gagcaagaaa tagacgacac gcctatgagt tacataaagc aggagttcat tatttcaaaa
     8701 gagagttatt tgactcttcg ttaaccatga ccaaggaaat tttaaagttt cttggttata
     8761 aacctgaaga aattgagaaa aaagccaatg ctttccaaaa acacgatgaa gaaacattaa
     8821 ttaaatcttt tgattttttt gaggaagaag ccagtctcat caatttctca cgtcaggcaa
     8881 aaggagaatt agaacgtatt ttgcaaaata ataatccata atctaatcat aaaatgtcta
     8941 acctcgcatc aatgtacaaa cttctgggat gccgatgata agcagtagcg agaaggtatt
     9001 gataaccgcc tatgcactag gaacaattaa ctcggctgat aacacactcc ttgctcaagt
     9061 ggcgctctag ttccagcaag caagcttgtt tttgctctat ataatcttta aaggaccaga
     9121 cctgtctttt gcctttgcct ggcatctctc gcaccgactc cttggcgtag gcggcagtat
     9181 ctgaataact aggggcatcc agcggtaact aaaacaagcg aaatcgaaac caagaccgga
     9241 atctactgaa agtggaaaaa atttctcgcc gccaggaaac ttaagagttc tcgaaaatgc
     9301 cctctcagtt tctaatacgc tctgtatacc cgagcaatgt acttgggcat cataagcagt
     9361 catttgcctt tgaacgtaac caatcacttt ttgccaaaat aaatcacggt tatcccaatt
     9421 agccaaagca tcaaaataat cgatataggt ctgatacgct gcgattaagt gctgcaaatt
     9481 aaaatgtttc ccttgagtaa tctctccttg aaaggctatt tgttttctaa atttttcaat
     9541 cgctgatagg ccctgatctt tatgactaat aatggctagt gcaatggcat tataatactc
     9601 tttgagatct tctgcagaaa cctcatcttc aatcccttcc ggaaattgtt catggaactg
     9661 ccttaatgcc tctccttttt ctaaccctgc aaaataaggg agcatcattt cccacatcgg
     9721 tttgtctcca gcaccaattg cggcctggaa agctgtacct agaatagttc ttcccgaata
     9781 gtccttggct tttgctttaa tcaagagcaa ctctgatttt tttctatcat agctctcgct
     9841 ttatcttgct cacccaaaac cacaagagtt aataattttt gggccgctct ttgaatcatt
     9901 tcggatgatg ccaaagagtt gagcattttt gaggctagcg aaagattata taaatctctg
     9961 gtcgtaaaaa agcctggttt aaattctgag caaatgtgca tggcgtcaaa tacttgttct
    10021 tctatagaag gttcattagt cacataattg ttataatcca tgcctgaatc atttcttagt
    10081 tctttatctc cttttgaata catgtgttta cctctataga ttattaagag gtcgaattgt
    10141 gctttaatta tgttaattgt tcaagttaaa cattaataca ctcgttttta agcacatctt
    10201 ccttcagatt tttaggctca gcatcattac taatagcaaa tagaagcatt caatctcctc
    10261 tcaacaacta gtgaatatag gcacatatgg agtgaattat gttttagaga ttattcctcg
    10321 atcaaatggt tattccgtac tagtgactag gcaacgtcgc tgaagttttt ttagacaaga
    10381 ttaaatctta aattgagcac agatttttca atttcacttc gatctgcccc ctctcccgcg
    10441 ctagaagctt taatagtatg atgatgcctt tcgcgggaga gggttgggga gagggaaata
    10501 gacaaatcta cgctaagaca acgagctcgt aatttacgga aaaacagcac cgatgctgaa
    10561 agacatcttt ggtattatct tcgagcaggt agacttggat ttaaattcaa gagacaagtg
    10621 ccaatgagcg catatatcgt tgattttgtc tgccttgaaa aacgactggt tatcaagctt
    10681 gatggcggcc aacacatgga taataaaatg tacgatacca agcgcacgga ttggctaatg
    10741 acaaatgggt ttaaggtctt gcgcttttgg aaccatgacg tttttcagca aacgccttct
    10801 gtccgcgaag tcatcatgtc tgcgttactt taatgccaat aatatactca caaacatcac
    10861 accagcgagg tgcatcctgg ctgttttgtc ataccttgta gcaatccttc taaaacgctt
    10921 aatcttctga aaaaagcgct caacaatatt cctttcttat aaacactttt ccagttgcca
    10981 tagtccggtg gcaagtcacg ccatgcagca cctgtacgaa gcatccaaca tattgcatcc
    11041 ataaatagcc tatcatcttc gccacgtcgg cctttaggtt ttggaagtaa tttttctaaa
    11101 cgagaccaca tctcgtcatc aatgaccatt cttgacattc cctctcccca accctctccc
    11161 gcgaaagaca tcatcatact attaaagttc ctagcgcggg agagggggca gatcgaagtg
    11221 aaattgaaaa atctgtgctc aatttaagat ttaatcttgt ctaaaaatct tcagggacgt
    11281 tgcctagtta tttgtagtga tttttacatt aattcgaggt catacccatc ataaaggtct
    11341 atacttccat ataatcatct tgaaatggct ttcattatga taaaacaatt acaacaggca
    11401 ttaagtgatg ttcttttagg aaaagaagaa gtcatcagat tgggtattac ctgtttatta
    11461 gccaatgggc acctgcttct tgaagacaac ccaggtatgg ggaaaacaac atttagtcat
    11521 gcattggctc aactgactgg tatgcaatat aggcgaatac aatttaccag cgaccttttg
    11581 cccagtgatc ttttgggagt atctatttat gatcatcaac gcaatgtctt tcaatttcat
    11641 aagggtccta tattctctca attaattctc gctgacgaaa taaacagggc cacacctaag
    11701 actcaaagcg ccttattaga ggcaatggaa gagaggcaag ttactactga tataggcaca
    11761 gaagctctcc cccaaccatt ttttgttatt gctactcaaa atccgtcttg ccatactgga
    11821 acttatcctt tgcctgaatc gcaattggat cgatttctga tgcgcctggt cattggctac
    11881 ccaccgccag atgcggaacg tatgctttta aaatcatcaa acttgaatgc tcaattaaag
    11941 gagctaagcc ctatcattaa tccacagcaa ttacaagttt tacaaagaga gagtcaaaaa
    12001 gttctagtct ctgaattggt tcttgattat gttcaatctc tactggacgc cagtagaaac
    12061 cagggatggt ttaatcatgg attaagccca agagcaggaa tgggattagt gcacgccgct
    12121 caagcctttg ccagaactga tgatagagat tttgttcgac ctgatgatat tcaggcagtc
    12181 atgccagctg ttgtgaatca ccgcttaatc ataaaaaaat cacaaaccca actctctgct
    12241 gccgaattat taatgagtga aattgaggtt cccgtgtgat aaggcaaatc aaaacaaaag
    12301 ttatggctta ttgggaagaa tggattgcca aacgaatctc taaaaccaat ccccaaattc
    12361 ttggttcaca aaatatttat attataccct ctggatttgg atgggcctat ggtgtggttg
    12421 tactgtccct gctttctggc gcaatcaact accaagttag cactgttttt ttaatgagtt
    12481 ttattttagc aattgtaggt tttataagtg cttgggaagc tcatgccaat cttaaaggat
    12541 tatctattaa attggtatcg gttgaggata cctatgaagg aacgccagcc caggttagct
    12601 tattgattca atcaaataat agaatgcgtt ttggactaga ttttcgcctt aataatcaat
    12661 cagtcactcg attagaaaaa attcctccac aagatttgcg atttatttta cctctggcaa
    12721 caactcgaag aggatgttat accctgccca aaatcaccat ttccagtttt tttccttttg
    12781 gcttgtttca ggtatgggga tacgccaatt ttgatagcaa ttattatgtc tatcctcaac
    12841 ctgtcaatcc tggcttttgg cccatacccc tttatcatca gaataaaagg aaaacagact
    12901 ctcatggaga taatgaatac tatgacttaa agcaggtaga caatccctgg attcaaccta
    12961 atcttattgc atggaaaatt gcagcaaaag gacaaggttg gtatttgaaa acgatggacc
    13021 gcgctgaaga aatgtattgg ctttttagat taaatgattt acctattgaa acactcgaag
    13081 aaaaattaaa acacttgagc tattggttgg ttactgccga atcacaaggc cagttttaca
    13141 aactcgagtt gggaaacgat ccagataaat tttcccatgg ggaagaacat cttcgatatt
    13201 gcttacgtca attggcggtg tattgatgga cttttttccc aaacaacata tttttctgat
    13261 aacaacccga tacgctcttt tggtcatgtt gatatgctac tttcctcatt ttttaacatg
    13321 ccctttgtgg cttactgtct tcatcttgac aacgctatgt tacaagttaa tatccgatta
    13381 ctttgcctat ccacttcttg acagcaagat acgctttatt gtggttatca tctgtctttt
    13441 tctattaaaa attcaatatg gcagcattat ctccagtggt ttttttatcg gttttttact
    13501 ggcatttatt ggattaaaaa ctattgaaat tcataatgat agagatatta gaatactaat
    13561 actgtgcaat ttttatctta tctttgcctc attaattgtg gtgcaggaac tatggatcat
    13621 accttattta ttaatagcaa ttctggctaa tttcactctg atgttaaaat taactgctcc
    13681 tcaagcctca ctaagacaaa ttagcggccg tagtatgaga ctattactga ttgcaacacc
    13741 attaaccgtc tttcttttct actttttccc tcgcatagcc aatccccttt ggcaagtacc
    13801 ttcccttggg aaaagtcaca ctggatttag cgaatggatg gaaccagggt cggtcgcaaa
    13861 tcttctaaat gatgacagga ctgctttacg aattatattt aaaagaaaag ccgttctaaa
    13921 tggctattgg cgaggattaa ctttaagtag atacaatggg ataagctgga atcctgcctg
    13981 gcatgactca tcaaccttct cttcattaaa ggaattaact acttctgatg acggggatta
    14041 tgaggtcatt atggagcccc accaaaaaaa gtggttgttt tatcttggtt accccaatgc
    14101 ggcttaccct caactcttat tttctccgaa ttttggtttg gttagtcata ataaagacat
    14161 gattgcccaa cgttttgctt atgctttgcg aattagctct tcccctcact cctcattgac
    14221 gcagaaagat tggaaactta atactcaatt acctaaatat tcaaatccca gaattacgtt
    14281 ctgggcaaaa aaacaatttg ccgaagtaaa ttatgacccc caagccttta ttcaattcat
    14341 ccagaattac ataaaaaggg aatccttctg gtatacccta actcctccaa aactggataa
    14401 tgaaaatgac caaatggatt atttctggtt tgattccaga aaaggatttt gtgagcatta
    14461 tgcaagcgca gtcactgttg tattacgcgc tgttggtatc cctgcccggg ttattgttgg
    14521 ctatcaagga ggtgaatgga atccactggc aaaatattta actataaaac aatatgatgc
    14581 acatgcctgg gtagagtatt ggcaaaaaga tttcggctgg aagcaactgg atcccactgc
    14641 atttatttcc acctcacgta tcgatccttc tgttcttgcc atgcgagcgg cgcgcaccaa
    14701 tcaacaatta ataattgatc cctcaaactt aaactggatt cagaaaacca aattatatat
    14761 ggagtcaacc cgttttttta tcgagcgttg gctgttattt tacaatcagg atgctcagta
    14821 tgagttatta gaacaaatcg gactaaagca ttggaatgcc tccacgctat tgcaaattgc
    14881 catattgtct ctgattttat ttatcattct cttgggcgcc tgttatcaat ggcggcaaaa
    14941 aaggacatta gaccccttat tgcgtgaata ccatctttta caaaaagagt tttgtcgctt
    15001 cgatattgca actcaaccac cagccacgct gaataaacag tgtaaaatat taatcaataa
    15061 aattcccaat ctggaagaaa ccatcactac ctttctttct cactatgaaa aactgcgttt
    15121 gcgacatcgc attaccaaaa ctaaagaaaa taaaaaacaa acgcttttgt tgtttaaaaa
    15181 tctgcgacga atattaaaac aggtaaaaat ctaaatttga cgggatttat cggttgcatt
    15241 cttgagtctc aaagtgctta aatagataac atttatcaaa ctatgatttt taataatggt
    15301 tcactttcaa aaaatgacag tccaaacctt tttaaaagac tattggcaaa aaaaaccatt
    15361 gatcattcgt caggccttac ctgattttac taatccactc acaccggatg agttggcagg
    15421 acttgccctt gaggaagaaa tagaaagtcg tctggtctat gaaacgcccg atcattcccc
    15481 gcagtggaat ttaaaaagag gcccgtttaa ggaatctgat ttgataggct tgcctaaaac
    15541 tcactggact ctattagtac aaggagtaga ccgtattgtt ccagatgtct atgagttact
    15601 tgagcatttc aattttatcc ctcaatggcg agttgatgac gtgatgatta gctatgccac
    15661 tctacatgga agtgtgggcc cacattacga taattatgat gttttcttgt atcaggctaa
    15721 aggtcggcga ctatggtcat taacctcaaa aaaatgccac accaacaatt ttataaaagg
    15781 tcttgaacta cgaatcatga aagaatttga ggtagaagag caatttatcc tggaagaagg
    15841 cgacatgctc tatttacccc cccatatagg acattatggc atcgctcaat ctgaagaatg
    15901 tatgacttac tcttttgggt atagaagtta ccaaggacaa gaattatggg atagtctggg
    15961 tgattatttg tctgaacatg gcttgttcaa atccctctat caagacccgg attggtccac
    16021 tcttaaaagc acatcggaaa taacgcccaa agcatgggac aatgcaagac aattgctaag
    16081 acaagtcctg gaaaatgatc agctcatgca atcctggttc ggttgttttg ctacaagcct
    16141 tgatcaatca gcagaacaaa atttaccccc tcctttagaa gaagatgagt tactcggttt
    16201 ggacgaattt attaaagaat tggctaatta tcaggaaata gttagagatg cctcatgtcg
    16261 ttttgcttat atcatgtctg accaagaatc ccagtgccat ttttatgtca atgggaaaga
    16321 atgggacagc cgtggggttt caacaaactt actcagtctt gttgccaata atcgattcct
    16381 gtctctaaaa gagttaaaac cttatttaag ccataagact aatcaattct tcctctatga
    16441 attatggaaa ttacaatggt tacaaatttt tcatgatcaa tgatttggtt tataaatatc
    16501 ttgaataaaa ttcaccctca ataggtagac aaaatatagt tttctaatta gtttattaga
    16561 acattattat atggatatgt aggataaaaa atggcacgct tttccagaat aagtttaagt
    16621 actgtattgt tatctctaat ttgtttcaac atttttgcct ctgacactat tatgattaca
    16681 gttaatacca aggaaaaaaa tgccattgct ataggttact cagtcaatgg aaaccaatat
    16741 ggaggtttgg gcagctccta ttctggacat ggacctaaaa ataaggagta tcaatttgga
    16801 tataagaaag attccatttt tggaaaagat atctcttgtg gttctatgac cttaaatcaa
    16861 gacagcaagg tgactttggt aaataataat aaccaatgca tcagcgtttt aggttaattg
    16921 gctattgcat ggaatgtaga tattaatttt gaaaagacag tagtgcctat ccaacaatat
    16981 tccgctcaaa ataattgaat tcaactcgta gaaactcaaa tctgtaatta acagtttata
    17041 agcccaagcc tgtctcctaa accagacatt tggcaaccaa aaacaccaaa aaacacaatc
    17101 aatacaatca attggataat tttaaattca tatagtatat ttaatataca tggacaaaac
    17161 aaggataggc aatgaaaaaa ataatattgg ccgtagttgt tatttctgct gctgtattat
    17221 tgagtggctg tgggggtgga tgctgcggtg ccagcaattc atgttgcagc tataatactg
    17281 gatgttgtgg tgtacaaaac tattccggta tttggtattg atttacaatt ataaaagcca
    17341 atagtctgaa atatgaaaaa aaacagcttg cttgcaatct cggaagaagg gtttcaccat
    17401 attgcttata ctgaatgggg tatctttgaa cctgagttac ctacagttat ttgtgtgcat
    17461 ggctatacac ggaattccag ggatttcgat gaattagcca attatcttag ctccaaggga
    17521 cgtcatgtat tttgcccgga tattgcgggt cggggtgata gttcctggtt taaaaatcct
    17581 cgccattata attttaccca gtatgtaaaa gatataacag cccttatagc aagggcaaat
    17641 gctcatcaaa tcgattggat tgggacatca atgggcggca tcattggcat gattttagcc
    17701 gcaatgccca attccccaat tagcaaactt gttctcaatg atatagggcc tcaaatacct
    17761 attcatgggt tgagaagaat tgctaaatat gcaggtaaag accctgattt taaaagcctg
    17821 gaggaagcca agcaacattt caaagccagc tatgctgatt ttggaattac caatgagaaa
    17881 cagtgggatg ttttcactaa aaacagtgta gaacaacgag gccctaatct ttatgtcacc
    17941 aaaatggatc cggcaataaa aagatcaaaa tcaattttac aaatcatttc agaatttttt
    18001 cgtcatcctc ataaagcact ggaaggaatt ttttatgaca ttaacctttg gtcaatttgg
    18061 aaacagatta agtgcccagt tttagttatt catggtgtgc attctgatat cttgacttcg
    18121 gagatcatca cacagatgaa aaaaactcat gctttaactg aagtttatga agtagaaaat
    18181 acaggtcacg caccagctct attgaatttg gctgatcaca aaaaaattga agattggctt
    18241 accactcatc aataatcaca aaaaaacgct catctttatt aagagttcta aataacttca
    18301 atgcaactca ttgattttaa tattgaagtt attttttgat atagagcacg ttatgcttga
    18361 aatgcttatg caaaacacca agatcacagc agaaaatatt tttaactcga ctttagccac
    18421 ttaagcagca aatgactgaa gcttcatctg acttttttgg tggtgaacgg ataaccataa
    18481 aaggcgaagc ccatggtctt ttacaccacg ggatgctccc ttggctttga ttttctttcc
    18541 gcggcaccgc tccagtgtct catcaacagc aataatcttg ggccatgatt caggaagcag
    18601 tgtgattaaa agaccaaaca aaattttact ggcagctaaa ccactccacc ttagctggat
    18661 catattgtct ctcaatcaag gacgttacaa aaagcactag agtaggttaa tcttatgatc
    18721 aaagatgggg tctctcccca gaagatcaga gggaacctac atctctgggc catgcggtgg
    18781 gtaagagcaa gagaaagttg gcagtaccag gagctacttg aatggttttt gaatgtgtat
    18841 tgggatttta accctgcggc ctacgccaca gggctgctca tccgcgccat taaaaaaaac
    18901 agtaatcatc atcaagtcca ggttttgcca agctcaggtt tttaagcgac actgtgagat
    18961 cggctgttct tacttcatcg aggtggataa tagccgctaa agcatccacg gcgccaagct
    19021 ttgccataag aggccccccc ggagccaccc cacagttgag agcaaaatca accccgagac
    19081 cagaagaatc agaaatagcc aaaggaaacc aagcccccac tttacctgta ttaaaattgt
    19141 agaaagttaa ctcgcgaggc aacttgtcct cattaaactg aggtgttggg tcaaacgaac
    19201 ggtctttacg gcaatattca ttagccacat gaactggaac atcacgctgc gctttaccta
    19261 cgttcatcca agcctcctta atagccgtcc agttctgggt catctcccag ctatcataac
    19321 catcaataaa atttttcagt gctgttttga gtggcgtaaa gtcaaaatgc tttgaattct
    19381 taacctcgac cccatgttgc ttaaatgtta acccattttt ctctatcgct tcacagcgtt
    19441 ttaacatagc tgcttttgta ttctcgtcca tatgcgattc aagcatccgg cacatgtgct
    19501 tgtcctttgc ccaataagcg tattcatacg ctgaaatatt ggtgaacgtt cgtccagaat
    19561 agtcggtcac tgtgccttgt ttaagcaata attcctgtag ctgctcggga ttttttcccc
    19621 ttattgtaaa tattttttct gcatggtctt gctttccatg agccacgaac tccagaaatt
    19681 tggataaaat agcagggggt tgaaataagg tacgggtttt acagtctact atactattcg
    19741 ttttgtcctt tattgttgat aagtaaccgg aaatttcctt tgctgtatct ggatataaat
    19801 ctttgattga ttttggcatt ttcgtcacct caatgtctat taatcgaact atagcataaa
    19861 aaatgcgcat aaaaatattt tcataaatat aaatctttgt gtgagaattc catgctatgg
    19921 ttgaaacaat gcgagtggag atatgccaga tatattgctt gaggctttaa gttatttcat
    19981 agccggtttg gaaaatgacc aaagtccagt ttaatgaatg agaacatata aattaaatga
    20041 ctgaattaaa aacatttctt aacaatgaga ttaagacttt gcgtaaattc agattatgtt
    20101 agtttaagaa attgaaataa ttgctgcttt aattgattta aattaattct atgactaaat
    20161 aaggtaatca aagcgcctgc caaaaccaag acgataccca acgcatgcca aggcttgagt
    20221 tcctcaccga gcagcactac gccaaagatc accacaaaaa caggttctaa aacggataag
    20281 atagatgcct tttccgaact gatgtacttc aagctgtgca gcattaacaa aatcggtata
    20341 accgttgcaa caaggcctat gccaaataaa ttcagccaaa cagggaatga tgttgggact
    20401 ttaaaggtct ggtcgaggca agctacaatc aaactggttg tcatacagcc taaacatacc
    20461 atcagggttg agacatttgg agaaatttca ttgtttttac ttgaaacaat ataacatgca
    20521 taaaaaaaag ctgaagccaa gcccagaaat ataccccata aatcaaatgc gatttcattt
    20581 acatcaatta acaacgccat tcctatcagg ataacagata tggccaaata gtatatcttt
    20641 ggtattgttt ggctatagaa aaaataattg aacaccataa taatcactgg ataagtaaaa
    20701 aatatcacca tcgataatcc ggagcctata tagagggaag cataaaagta cagtaatgtc
    20761 gataaaccat aatagaggac accacttaaa aaagcggtaa acatggattt ataggtatca
    20821 ttgatatttt ttaattgagg aatcaaaaca atcaaaataa aaatactgga aataagaaat
    20881 cgccaaaaca acatattgct tgctgataaa tcaccatgga tagcactaat cccaaaataa
    20941 ccaataaatc cgtaaagaaa tccggataaa atagcataaa aggatccacg ttgttcttgg
    21001 gagttcatga ttgagttact cgaaataaga ggtatgatct aacaaataaa aagtattatt
    21061 ttactacttt tattctattt tgaaaatcaa taattcaaat ttaaatcaaa ttgttaattt
    21121 ttaacccata aaatagcgac tattcatcac tcgacaaact gatatcccac aatgttttgt
    21181 acagtttatt taaacacact ctattgacaa caaaatatac acaatgtacc attcgaaaac
    21241 ttcaattttg gctctatatc aactaatgtt tcgagttttg atttttctat cttgcctagt
    21301 aattttgacg ccaaacgcta ttgctgacac taatccctgt gaaaaacact cttgtatagc
    21361 tgtcattgat gccggaagta ctggttccag gctacatatc tactcctatg atacggatga
    21421 caccaatact ccaattcata ttgaggaaat ttggaataaa aaaatcaaac ctggattcgc
    21481 aagcatacag cctaattctg taacaattga tgcctacctg acaatgctat tggctgatgc
    21541 accaattcat aatatacctg tttactttta cgcaacagct ggtatgcgac tacttcccca
    21601 atcacaacaa aaaaaatact atgatgaatt ggagtactgg tttagacaac aatcgcaatg
    21661 gcagttggtc gaggcaaaaa ccatcactgg gaacgacgaa gcattgtttg actggcttgc
    21721 tgtaaattat aaacttgata cattaaaatc agtccaaaat aaatcagttg gtgtcatgga
    21781 tatgggcggc gcttccgttc aaattgtctt tccaatgcct aaaaatgtgg agattagcaa
    21841 acataaccag gtagaactca atatctatgg gcagaatatc aatctgtatg ttcacagttt
    21901 cctgggttta ggccaaacag aaatgtcaca tcaattcctt aactccccct cttgctttgc
    21961 aaacgactat cctttgccgg atggggagtc aggacagggc aatgctccat catgcaagga
    22021 agaagtaacc tcattaatga atagtgtcca taaggtaaat caacaaatac aacctttgct
    22081 tgctttaaat cccgtgaatg aatggtacag tattggtggt atttccaatc ttgccagcag
    22141 ccaattattt cattttgaaa acagcgagct tactaatcaa agtttgctgc aacaagggga
    22201 caaccaaatt tgccatcagc aatgggatat acttaatggc cagtatcctg acgatgagta
    22261 cctctaccaa tattgtcttt tatcctctta ttactacgca ttaatggttg atggttatgg
    22321 cattaacccc aatcaaacca tccattatat tccgccagaa caaaatctcg attggacaat
    22381 tggagtcgtg cttcaccgcg cttgaagcat tacaattcat cgattgaaaa atcatcaatt
    22441 ctcaggggag aaggttgacg gtaaaaagca cataaagcat tacgaaaatg tagtgcacgc
    22501 tgtggtaacc cctgggtaca ataatgaaac acctgcttat agattgactg tttaaaggcc
    22561 tccgtgtcta cttcagcccc atttaaatta tcaatactta tcctaaattt atgtcccgcg
    22621 gccatagaaa gaatccactc taaagcctgt ggcttcactt ccaccctctg aaacagagtt
    22681 tgttgttcct cattgcgtcc atcaggagcg taccaatagc caaaatcgac tagttttctt
    22741 ctttcttctc cagcaataag ccaatgagcg cattcatgca aagcactgct aaaaaatcca
    22801 tgtgcgaaaa atagcgcatg ataaggcctc tggtcatctg caggaagata aataggctca
    22861 tctccccctc taaccagacg ggtattgtag tcattctcaa agcaataatt aaaaatacta
    22921 attagatctt ctacgtgatg cacgaatcgt tacctagcaa aacctttgat gcttacctat
    22981 ctgatactga ttatactctt ttttcaaagc agattttaat tctatttcat tttccatacc
    23041 tattttagct gccaaagtta aagaatgctt gatatgcaca gcctttgcct tgggtatctg
    23101 aagagaaatg acttcgtcat gcgaggcagg attcatgctt gctagtaagg cttctttttt
    23161 agatagccct cccaaacgtt tctcgggttt atccttaaga ggaagaaact ctttttgttt
    23221 cagaagacaa cgagttacct gataataggt attaatttgc ttataagtta aatcgatcgc
    23281 agtttcgttc ttatagatgt ccatttcagg atttctgggt gtagatggat gatgaagttt
    23341 ggccggtttt atctcattct catccggtac cttttcgtta ccttttaaaa tgaaatcaaa
    23401 catccctgcc caagtgtttt tgtcttcttc attactactt aagccaaaag acaaataaaa
    23461 aattccactg gatattgcaa aagcagcaag agtacctatt gaaacaatag cgggaataat
    23521 tcctgcggta cttaacccta taagagtacc aatagtcagt aaaggaataa taccgaatac
    23581 gattaacggc catttctctg caatatagtg tattactggt ctgattaagt aggtataagg
    23641 agccaggcca aaataataaa gaacccctct ccctaaagag taaagccaga aattccttgc
    23701 tttccttttg gcattttgtt gctcagcaga aacataagta aattctgttt cagcaaatcc
    23761 ggcataattc agaaaatgat cacaaaaaag attggggcct tgtttatctt ttggaggctt
    23821 gacatgtaaa atgttccact cttttttcca aactccaaaa aaagataccg gatcgttacc
    23881 ttgctgttgg acaacaacca aaggtttctt tttgatttta tcccatttat cgtacttcac
    23941 tttattatca gtatcatgaa gtcctgcagg gtttaaggca tccactctgg aaaggtattt
    24001 tcccatatca atagccaaaa gcagacttaa agaacctcca aggcttatac cacacacatg
    24061 aattttactg ttttgtttta aaatccagtc cttaattcta tctcttccat ttttatataa
    24121 agaatagcca accgtttcaa atcctttcaa atcagtattt aattgggaaa taaagccttg
    24181 acctgcagga taagtggttc ccataaaaat taaatgaggc tgggcatttg gattatctat
    24241 tggctcaagt ccataagcaa aaaccctatc atcatctcgc aaaatcagat cattttttcc
    24301 acgatgattt gtaagttcta taggcttgat gtaatagtca accataaccc attgatcatt
    24361 gattctctgg ggaactttaa tcgactcgta aggatttaaa tcaaataaag gcaaataact
    24421 aagacagtta ctaaggtaaa gttgtatttg ttcattttgt tgcgaattaa attgcctgcc
    24481 acttaattgg gtaaatagtg catcgaaacc cttctgaaag gctaatcgaa attccttcag
    24541 caacagagga tctctttgaa ggtaaatagc cttaaaggtt tcccaggaaa ataattctgt
    24601 ccatgaagta ttccaaccca tcattaacaa tcgcaaagta ttacgaataa gaacggccct
    24661 ttgctcttca ggaggtatgt tttttctaga ttcgaactca gcattctcaa taaaatttaa
    24721 attcaaacca cctttaaaac ttgtacactc cctaaccata aaaaacctct aaatagcttg
    24781 tttataacta tgagttcaag acaattacaa ttactggctc gcagactgct aagcgagtca
    24841 tttaaaaacc aagtaactgt atatcactat caattaaaag ttggaatttt agcacaataa
    24901 caaagattat tgctaacaat ttaaacccaa agggtttatt ttgcccaaaa agatgggctg
    24961 tctaacaaga aagttaaacc cccatacttc aaggcattgg atataatgac tcttactccc
    25021 ccttttaaaa aatactatat agaagaagca aattgggttg caatgaatta ataacacaac
    25081 cagcgctata atttttcttg ctgtagtgac ttggcaactg aagggcgagc caacataaga
    25141 tccataaatt ttgtcaaatg ctcaaactga gacaaatcca gtttgaaata ttttgcccag
    25201 cttaccataa caaatagata ggcatcaggt aaactaaatt catttcccat caaatatgat
    25261 ccttttgcca aacgagtatt ggtataagcg aaacgggtca tgactaaagg tgtcaaaatt
    25321 ttggatttta tttcctctga aacagtagga ttaaaaaaca taccaagcga cttatgaagc
    25381 tctgtagaaa tataatttaa ccattccaga gtatgatatc ttttactctc tccaacagca
    25441 gccaacaatt tttgtcctgg agtagtatct gccagatact gtagaataac ctgattttca
    25501 gtcaggacat cgccgttatc aagaaccagg gttgggaccg ctcctttagg attaatttta
    25561 aagaaatcat cgccacctgc tgtttttttg actgttaaat cgacctcttc atcctggaag
    25621 tttaacccta gctcattgag tataatacgg actactaatg aacaagcacc tctgcaataa
    25681 tataatttca tgttcactcc tttgatgatg tttaaataat aatagctaag ttagcacaaa
    25741 atgcagcaaa aaacttgaaa tgtaaaaaac catacagtga aaaaggatga tcttgtatgt
    25801 ggtttattta atcagaccac ttttaatcag gctctataag aagcaacgat tcgatatgct
    25861 ctaaccctga tattatcata acaatattta atatgggcac ttggcgcatt tgagatcttt
    25921 ctcaatttca ttgattaatt tcatagtagc tgattccctg aaaataaaag aatcccaaaa
    25981 ataagttctt cttaatgttt gcaaatcaac tcgatttttt tccttccatt gctgtatacc
    26041 aaacagcaat tcatcaatag attggttttt ctgtgaacca atgatttgca aatataaatt
    26101 ttttaagcta ttaaattttc gttttgtcat tggtgaatta ggctcgagca caggcaactg
    26161 ctctattcta tgatagagtt ttttggctat taagcatttt tctttattaa atcctggatg
    26221 gatagaaggt ggaggtgggg gtaaaacata aaagggaata ttttgactcg gtttgccata
    26281 gtctaattga gttttatacg gcaggtttat aaagaaacaa gaggatatcg atgaggggac
    26341 agatgttttt gtaaccgcct caattaattt tattgctgaa tgtcgacaag tattacctgt
    26401 attgatttga tgaatttcct cattaagctc cttccaatga gaaccagttg cgaaaacacg
    26461 agaactggtt tgcgaaaaat agactgcatt tccattttga acaagcggtt taaaacattc
    26521 aaattgattg ctctcagttt gaatagactc aagataatga acaaattcac aatactgctc
    26581 ataactaata tcataggctt gataagaaat aggtacagat ccttttcgtt ttcttgtaat
    26641 tccttcatcc tctatctttg ctttgctact cgcaaaaatt gccccaccta aagcactata
    26701 aatgctaaga caccttttag tacccttggt ttgattaggc agatcaaaga ttttccctac
    26761 tctgcacagt agatgagata cctggaagtc atcatacaca cctagcataa taaatgagtg
    26821 ctgattgatt ttacctatcg aaataaaaaa gtgatctgtt tctttattaa tacagattgc
    26881 catgtgattc agtccttaac aaccaataat gataaataat aaattagctt gcacattgta
    26941 agtgtggatg gtttttaacg ataactttat gtatattcaa acaaagctca tggatatggt
    27001 tgcccatttt tgatttctca ttgcctgagc cacctttccc attcaaagcc acaacaaccg
    27061 ttacattatt acaaggattc cacaaataca tcaccctgta acctaaagta ctgccctggt
    27121 aagtccaaaa gcgctgcctg gaatttttat cataataaaa accaactccc aatccaaaac
    27181 cgtagggctc atcttctgtg actgaaggga taggtaaccc tgtttttatt gaaaccactg
    27241 actccaattc ggataaagcc tcgtctctaa aagcaggctc tatcagtaac ccatgatata
    27301 gcagctgcac ccaacgaccg acgtcttcag tatttgcgat aacagcacct gctgcgccag
    27361 cccaggataa atcattgctt atagtatcaa ttagtttttt gttgtcctca tcgtaaaaat
    27421 aaccatggac tcgtctatcc ctgattgatt tttcgacttc tggaccatgt ggtcctgcag
    27481 gataaaatga attgtttaat ccattgtttg tattaataat cctctgtttt aactgatttt
    27541 cgaaagtatc ttgagtcact ttttcaataa ccattccagc aagaatataa ttagaattgg
    27601 aatactcata cctgtttgct ctgtcggttt ctattggctt ttctggatga gcataaccta
    27661 acaactcctc atcgccccag attgccgcaa aattatcctc catctttttt gcaaattcag
    27721 gatcttcgga ataattgggg atgccactgg tcatattcaa taattgtctt aattttacat
    27781 ctttccactc tttgtattga ggcagccatt tacccaaagg atcatccagt gacaatttgc
    27841 cctcagtctg caattgaagc aaaatcaatg aagtgaatga tttggtaata cttccaatat
    27901 caaacaaatt caatggggta atttcctgag acagtggagg atatcccata gttcctttga
    27961 caaaagtggc aacatccaat gcatcattat ttttctcttg aggaataaac actgagacag
    28021 caatggcagt aaatttttct ttgttatgat aaaccttgaa gtacttgctc atttcctttt
    28081 gaattttatg atgagataca acaggagatt cagctgcgct gatattataa gataaagaag
    28141 ccaggcagaa ggcaagcaga aaactaacta agttatttat tttcatggcg aaaaatacat
    28201 caagttaagt agaaaattgg ccattttaac ctgatagact caaatagatc cataaaataa
    28261 aaaaaacagg agatgttctg ttgaatatac gggcacgcct attcgcgttc tcttcggcca
    28321 agagcaatga aatcgagcga aattgtagtt ttcattgccc acttgaaata tgaatacttc
    28381 tccaaataca gatctttttg cattcagctt aaaatcaata aatatttaga ttctaaacag
    28441 cactggctct ggctcgtatt ttctccaaag cgtcttcaag acgtttgatt gatttgtttt
    28501 tacctaacaa agctaatgtc atgtcaatgg aaggagatgt tccggatccg gtcacggcga
    28561 cacgcaacgg ttgagcgatt ttgcctaaat tcaaatcaaa ttgcaggctc acatcattaa
    28621 tgcattcttg aattttatca tttttccatt cgtcaagcgc ttttaggcgc tcatgcaata
    28681 ccattaaagg ctccagaaca actggccgca agtgtttttt aacagcatct tcattatatt
    28741 caatggtatc tgtataaaaa tactgactaa tttgacacat ttctgctaat gacttacatc
    28801 tctctgcctg aactgcgacc aaatccttca aatcaggacc ttgattcaaa tcaattcctg
    28861 cctgctcaaa atgccattgc aacgcattgg caactgattc ttgagagtca cttttttgat
    28921 aatgctgatt caaccaatac aacttatcat aattgaaact tgagacacct cgactgacat
    28981 ttttcaagtc aaagctgttt atcatttctt gtacactaaa aatttcctga tctccatgag
    29041 accatcctaa acgaactaaa taattcaata aagcatgagg caatacaccc agctccttga
    29101 actgcaagac acttactgca ccatgtcgct ttgacagccg tttgccatct tcacccaaaa
    29161 tcatgggcaa atgggcaaaa acaggtaccg gcgcatccaa tgccttgaat aaattaattt
    29221 gtctcggagt attgttgata tgatcatccc cgcgaataac atgggttatt ttcatatccc
    29281 aatcatcgat aactacagca aaattatagg tggggtgccc atcagaacga accaaaatca
    29341 aatcatccag ttcgctatta tccacatgaa tgtcaccata aacctcatcg tggaaagaca
    29401 ctatacccgc atcaggattt ctaaagcgaa taacataagg aactccacta tcaggaagaa
    29461 tttggtttct gcaatgcccg tcatagcgtg gcttttcctt ggcggctaat tgagcctctc
    29521 ttaaagcttc cagtctctcc ttactgcatt gacagcgata agcttttcct tctttcaaaa
    29581 gttgctgcgc tacttgttga taacgagcat atcgctcagt ctgataatag ggaccttcat
    29641 cgaagttaag ccctaaccag gccattccgt ctaaaatagc ctgtacagat tcttgagttg
    29701 agcgctctct gtccgtatct tctatacgca gtataaattg gccgttgtga tgctttgcat
    29761 atagccatga aaataaagct gtccttaccc caccaacgtg taaaaaacca gtagggcttg
    29821 gggcaaatcg ggttctaaca gtcataatat cttccattgt aaggaataaa ataatcaagt
    29881 tataatagcc gagtttacca tgaacgcaaa gattcaactg ctgaattaag actaaaagga
    29941 gctatgttga aaagtattgg tattaaatat cagctaagaa tcactactct tattcctgcc
    30001 tttttagtcg ccttgctttt tgcttttttt tataacggcc tatttggtaa agatttaaag
    30061 cagcatatgt ccagactggg tgaagcctat attaggcaat tacttccagc agcccagtat
    30121 gccatgcttc gcaatgatta ccgtacattg cagggtttaa tcaatgcctc aaccattaac
    30181 ccggaggtaa aagctttggc attttataat gctgatggtc gattaattgc ttatcgtggt
    30241 ggcaagcatt ctattcataa accattcaac ccacctgact atacaggtga ctacattgaa
    30301 agtaagcaaa ttaacccatt caccattaat ttcatagcac cgattactat cccaaagttc
    30361 aatttgtact ccagcacaga gttcaaagag ctttctacgc ctaaaatatt ccaggctgac
    30421 gatatattag gctggttatc tattgacatt gatacacaat cactacttat aaaacgctat
    30481 caaatgctca tcgttaccat ttttattacc ctattcggtt tactgatggg tttaacaatt
    30541 cattacttct tgtctaaacg aatttacatg cctattgctc gattgcgtcg aagtatgaag
    30601 caaattttaa gtaatgaatt tgaaactgaa attcgtgttt caagtccagg agaattagga
    30661 ataattgaaa aaggttgcgc tcatttacaa cgacaatatt taaatacagt tcgtgatttg
    30721 aatcaccaca ttgaaatcgc cacagcagat ctgcaacaaa gtctagaact tctggaagaa
    30781 aaaaacattg agctatcact ggaaaagaaa aaaactgaag aaaaaagtcg acaaaaatca
    30841 gaatttattg ccaacatgag ccatgaaatt cgcaccccaa tgaatggcgt gattggtttt
    30901 acaaacgttt tattggaaag caagctggac cctttacaac tggactacgt taaaacgatc
    30961 aaatcatcag cgcaggattt gttaagtatc atcaatgata ttctggattt ttctaaaatt
    31021 gatgcgggca aattaaatct tgattgcatc ccgctagaca taagaggttg cattgatgag
    31081 gttttatccc tggcaagccc caatgcgcac aaaaaaggaa tcgatttaat cccgattaca
    31141 gacatcaatg tgcccaaaat ggtattgggt gaccctttaa gaatcaaaca aatcatcagt
    31201 aatctggtga ccaatgcagt aaaatttaca gatcatggat acgttcttat acgcactaaa
    31261 atcgagcaag aaacagataa agattatacc ttgctatttg ccattacgga tacgggaata
    31321 ggtatttcac cagaggatca aacgaaactg tttactgcct tcaatcaggc agacaccagc
    31381 atcacacgcc gttacggtgg ctcgggatta ggcttggtta tatgtaaaaa actatgtgaa
    31441 gaaatgcatg gacgtattag tctaaccagt gaaatcaaca aaggctctac ttttagcgca
    31501 cgcattaaag ttgaaaaact tgttgcttat gagattgaaa aaaatcaaac tcatcgtttc
    31561 gcccatttaa aaatcatttg ctttgatgac aatcccctcc atttggaagc aataggtaat
    31621 ggcttgggat tttggggtat agaggctata cgcgttgact catttaataa actttcccga
    31681 atattgacaa aacataaaga ttgcaaaatt gcctttatta acgtcaatca aggatgtgag
    31741 cgacaagctg ctgaactcat tgccaaacat aagcaaatcc ctttcgtgtt aatttccaaa
    31801 tggcctatta atgattttgc agcgcttggt gccaggggtt ttctttacaa acctattagt
    31861 atccaaaagc ttcaggatct gattgaatcg atagccaatg aaaaccaaac cgagaaaaat
    31921 acgaaccagg aactggatac tttacgcgaa cagcttcgtt ttcttcatcc agagatactg
    31981 attgcagaag acaaccctgt caataaaatg ctattaacat ccctattaaa caataatgcc
    32041 aatataacaa cggttgacga tggcgaaatg gccgttactg cttgtgagga taaaaaattt
    32101 gacatgattt tacttgatct gcatatgcca aaacttaatg gtttggaagc agccaaaatg
    32161 atacgtcaaa aatccctaat gaataaacac tcccctatcg ttctcattac cgccagtagc
    32221 agtgatctga gttccatcga tatgaaaaaa tccggagttg atttttgttt tcaaaaacct
    32281 atagatgaaa agcaattgtt aatccaaatt cttcgcatcg ttgataaaac caaacatgct
    32341 gccatcgatt ggcaattatg cgttcagaaa gtttcaggta atcaagcgct tgctgaagag
    32401 ttcctcgcca aattcataga agaattatat aagaatcgtg aagagtttat tgagttaatg
    32461 catcaaaaaa acgtaaaggg cctggctgat ttggctcata aattacatgg cgcctgttgt
    32521 ttttgtggtg tacctatttt acaaaagaga gtagctcaat tagagaagct tgcaagacga
    32581 acagctaatg ctgataattt aaccggagcc tttaccgatt taatacaaag tattgatgca
    32641 gtgattagtg aatatgaaaa tcagtattca caataatatt taatgaaata aggccataaa
    32701 aaactctctt gtatgaaaac caaagtaaaa atctgtatca acctaataaa atcattctag
    32761 ttatggagac aaaatgtcag ttaaaaatgc aatctacgcc cagtctggtg gtgtgaccgc
    32821 tgtaattaat gcatcagctt gtggtgtcat ccaaaccgcc aaactatttc cagagcaaat
    32881 tggtaaagtt tatgctgcaa aaaatggcat tatcggtgcc ttaaacgaag agttaatcga
    32941 tacttcattg gaaagtgaag aagacattgc caaattgatg caaactcctt ctggggcttt
    33001 tggctcctgc cgctacaaat taaaagacaa tggggaagaa tatcaacgct taatcgctgt
    33061 cttcaaggcc cacaacatag gctatttctt ttacaatggc ggcggggact cacaagatac
    33121 tgcccataaa gtatcccagt taggtaaaac catgggatat cctattacct gcataggtat
    33181 ccctaaaaca gtagataacg atctgccttt tacagacaat tgcccggggt ttggatcagt
    33241 cgcgaaatac attgccattt caacaaagga agctggtttt gatgtcgcgt ccatggcggc
    33301 ttcatccacg aaagtgttta ttcttgaggt gatgggaagg catgcaggct ggatagcagc
    33361 ggctagtggg ctggccgagc aaaatccagg cgaaccgcca cacattattc tattacctga
    33421 ggttcctttt gagccaactc gttttctaaa aaaagtgaag gactgtgtca attcatatgg
    33481 ttattgtgtg attgttgttt cagaaggtat ccgcaataag gaaggaaaat ttctcagtga
    33541 agcaggatta cgtgatgcat tcggacatgc tcaattaggt ggtgttgcac ctgtcatcgc
    33601 tcaattaatt aaatctgaac tgggttttaa atatcattgg gcagttgcag attatcttca
    33661 acgggcagcc agacacatag cctcgcaagc tgatttagat caagcttacg ctttaggtaa
    33721 agctgctgtg gaatatgccg ttaaaggata taacgcgatt atgcctattg tcattcgtga
    33781 acaagacgaa ccctatcaat ggtctatagg ccaagtccct ctggctgagg tagctaacca
    33841 ggaaaaggct atgccaccag agtatatagc tgaagatggc atgggaatta cggacgcatg
    33901 caaacgttac ttgtcccctt tgattcaagg tgaagcttat ccaaattaca aaaatggatt
    33961 gccagattat gtccggctaa aaaaccagtt agtggctaaa aaaatcaatc aagattaagg
    34021 gcggcagtag gctggacgat atttactaat cagggtaccg ccagtgcaat cccaagtgag
    34081 tattctgtta gtagcatcca gggtgggaac cattgtaata gtgccattac ctgcgtttgc
    34141 agtgtaggta atggtaataa tgccttgatt accaatggct atagaggcta cattaattgt
    34201 tggggctgga ctggtataac ccgtatctgc ctgatttgct ggcaaagtgt tatcaattaa
    34261 actcgtttca acaacagcta atttagcggc cgaagccatt tccaatcctt caaccacccg
    34321 tgctctgatg gtatagtctt gataggctgg cattgcgatg gcaaccagaa tcccaagtat
    34381 agcggcaacg atcatcagct cgattaaagt aaatcctttc gttctcataa cgtcacctgg
    34441 cattgcatct aactgatatc acgcaaattc aatgccaact ttatgattta aaaaccttct
    34501 gctgactaaa tagcttgaca agctttcttc tcaaaatatt cctgtcaggc aaatagcttc
    34561 tagaacttag ccaaataaaa aaccgccatt gaaaggcggt ttttatagtt gatttatcct
    34621 attatggtct gcagctggca ggtcgatatt tagccaacaa agtaccaccc gtacaagtcc
    34681 aggttacatc accgttagct tgtaaggttg gggtcataac tatggtacct ccacctgctg
    34741 cagcagtata cgtgatggta atcacaccgt tcgcaccaat agcaattgat gccacattgg
    34801 gcgtagcagc aggagacaca tagcccgttg cagcctgagt agctggtaac gcgttgtttg
    34861 taatagccgt ttcagacacg gcaagttttg cagaatctgc caaggtcaga ccctcagtaa
    34921 cacgagctcg tattgtgtaa tcttgatagg ctggtatagc gattgctgcc aaaataccga
    34981 taatcgctac gactatcatc aattcaatta aagtaaaacc cttttgtctc atgaccatct
    35041 ccatagttaa ctatccatta gtataatgca agcataatgc caactaccag ccaatattct
    35101 aaacctatat tattttacat gttaaataat catttcatgt aaaaaacgcc gttttttgtg
    35161 ctaaaaattc agtgaaatga atagataaaa tatttcataa aacaaactat ttgttattaa
    35221 ataataataa aatgacagt
//
LOCUS       DAXLDT010000015        33410 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXLDT010000015 DAXLDT010000000
VERSION     DAXLDT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 33410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33410
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.015"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            237..1328
                     /locus_tag="ACF2SP_000602"
     CDS             237..1328
                     /locus_tag="ACF2SP_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HFD2358408.1"
                     /translation="MKKYHQLLMGITLLLFGVCNYAGDTGPVENSDLFRVFIPNLKPG
                     YDFSATAFYLQPGANNLGWGVITTVLPIPSPNWQVVTFNPDYQVGFNLGGRYVFANSG
                     SDLQLNWSHLRTNDTDNVFVNPSSQWISPFSQTGTPPTPSGQITGVALLKLAHASLDF
                     SYDAVSLDVGKFINFGSKLQTRLFTGLSSAWIKEELISTFRGFSLPILSLNNTSTYKG
                     VGPRLGFNNSYNISHGFNLVGQLAGSVLYGRMQPAQYQFTGTSQQLIIAGIFVNREGL
                     ANPSVNQLVPALDAKIGLSYLYSLKQNWDLNFEAGYMGALYFNPLSSYETNTNVIALD
                     TGSLSTSSAKHTQSNFSVGGPYITVSLRT"
     gene            complement(1488..2060)
                     /locus_tag="ACF2SP_000603"
     CDS             complement(1488..2060)
                     /locus_tag="ACF2SP_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFD2358409.1"
                     /translation="MSQNIILNRCEWVGQNKPHYELYHDTEWGIPVHDDEKHFEMLIL
                     EGAQAGLNWETILKKRDGYRRAFKQFNPQAVAQMTDDELNALLTNPEIIRNRLKIFST
                     RKNAKVFLSIQQEYGSFDSYVWQFVNGAPIINRPESIRAIPATSKESDALSKDLKKRG
                     MSFVGSTIIYAYMQAIGMVNDHIVTCFRAK"
     gene            2377..2757
                     /locus_tag="ACF2SP_000604"
     CDS             2377..2757
                     /locus_tag="ACF2SP_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2358410.1"
                     /translation="MTQAAFGEFCWNELATSDVKKAKDFYGKVFGWQFREIESKEMTY
                     TLIQCNDKEMAGIWQIPKDQQSHIPPHWMAYIYVQDVSEALKKAKQHGAQEVKGITQA
                     GEMGRFAIIVDPTGAHVALWESFE"
     gene            complement(2858..3811)
                     /locus_tag="ACF2SP_000605"
     CDS             complement(2858..3811)
                     /locus_tag="ACF2SP_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HFD2358411.1"
                     /translation="MARNKRLDRFHNAQNSNTCGYDQAFSEIQDGRKRSHWIWYIFPQ
                     LENLGKSPSAKLYGIKDFNEACDYLKDPVLFKRYFELIKLIEEKLKNIPLPHLMGGFP
                     DDRKFISSLTLFQATSNYLSSKDTDSQNKYHELYETCNRVFKQIAPQGYSPCQQTELL
                     VKHQSNSPVKKTTEKSSQPPQVTIFSPKRSKPNTAHPILKDLAQYINKRGNEWSYHYN
                     FLGLVAFVYFIQDLILGTDHFNHKAREVKLTAASKLQKILDPQCHERCNLNPSERKAL
                     SDGRLGEIINKHGGLNKILTEAPDKPPKNDRAQEINSNPFL"
     gene            complement(3922..5346)
                     /gene="sidA"
                     /locus_tag="ACF2SP_000606"
     CDS             complement(3922..5346)
                     /gene="sidA"
                     /locus_tag="ACF2SP_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector SidA"
                     /protein_id="HFD2358412.1"
                     /translation="MSKKYRTPIRLSDKMKTLKKNLQEQLTQSKSLGEQIHAGTTTAS
                     TITTGINHLFNVIASFLSSYRQIPLVGGVLQLVAILAKSISIVSDPEKSIAEKIISSI
                     LIAAIIALSIAAITLGGLAAAIIGTVIASTVTIMEGLGFFGKIAEKFQLANSYKMKKE
                     FIDLVAKRIEPNNDKYNDLFEIRAIELQHELEKSYPNQIEQQQLKEELNFINAVLRKK
                     NITPGNNKENDAYKLNQLYAKRAEQLKTLVDKIALINPTGKRAEQNKLLSEIKALQKE
                     ITKTDDSIEEIIAPLAKIERDNLKSTINLPLSYSTFAIAAASTLISIAGLLVVAGVIA
                     APPIMVPIMFAVGSVMAIIGLIKWTAEKMAEIEDAQLKAEKTAKHEDAVLDEALNVYE
                     QQLNLELVSPSNPARQVKNILIVHDKVPALSPTESTVSSLDSRFTLFGSSSREHTKIA
                     EQKTTDTTNEQVPTTFDSISKPTR"
     gene            complement(5507..7435)
                     /locus_tag="ACF2SP_000607"
     CDS             complement(5507..7435)
                     /locus_tag="ACF2SP_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358413.1"
                     /translation="MTRAQTCPHQTTVISNLKIIPLTLGWLLKSALLIIIIAVFIPLS
                     PKMPAPGLDASWAVGLNQAVAQGLAFGKDIFFTLGPYSSIYTKFYHPATDFMMVGGSL
                     YLALCYWIAIILLMQGIKWRWSIAFAILLFSMIYARDSLFFSYSLIIGLLIHKEISGK
                     ETSFKSNFCTLFALLFTPFGLFPLIKGSLLILSIITVLLCSLFLILYKQRNLAVLCLA
                     SPVVSILFFWIASGQSIFNLPAYVSNTILLASGFTEAMAIEGDNKELIFFGVTSSCIL
                     LSILLQKQLSPLSKAFLFCIYLVFLFLSYKTGFTRHFGHAFIAGTSVLLASFLLPYFN
                     SNNKVIVPLLALSLYTASYINGHYTKISVRDNFISTYTAAYYGLKNRIQNKNWLKENF
                     SLTMNYLKARDDFPRLPGTTDIYSYNQTSLIASGMTWSPRPIFQSYSVFTAKMAEINT
                     NYLLSENKPDNILFKVEPIDNRIPSMEDGMSWPILINHYQPVRLENDFLFLRKKLVNK
                     PVTSLVPLKTEIHTFGEQVKIPDTDKSLFVEIDIQPTLWGMLATTFFKPHQLEANLEL
                     KNGTQRQYRLISNMAKSGFLLSPFIENSAEFGLLFSQNNYLAGKKVISFSINSKQVPS
                     SHWQTKYTIHYKTFASNN"
     gene            complement(7561..7953)
                     /locus_tag="ACF2SP_000608"
     CDS             complement(7561..7953)
                     /locus_tag="ACF2SP_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358414.1"
                     /translation="MLTTFLATVIGWYLVIVSLFLLFHNEHAKSAMAEIIAHRGSQLI
                     MAIVTLITGLLLVVSHNIWVMGWPVVITLFAWLVLLGGLIRFLCPDTAVRIGQSFVDN
                     PMRLKIAAVIFFIIGLFLLFNVYYVYLK"
     gene            8090..8467
                     /locus_tag="ACF2SP_000609"
     CDS             8090..8467
                     /locus_tag="ACF2SP_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358415.1"
                     /translation="MMKKINLFKLLMIFVLGTSALQAAGLSTQRIEQLSNARVTVWKT
                     IIYPSSKQVLPMHRHDNDRVLVALTDGTLKITNNKGKVHYLKLKKGKSYYLTKDAPNE
                     LHNDENVTNHPIQVMVIQLNDKA"
     gene            8668..9975
                     /locus_tag="ACF2SP_000610"
     CDS             8668..9975
                     /locus_tag="ACF2SP_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD2358416.1"
                     /translation="MHTQRGLEVTTGSIEAVDSINYFHEQVLSSGQNAIQILDSAKKH
                     TDNLLIQTYAAAFYLYAQEDVANEQASDYLQSAFRLLTSANEREKLIFEAVTNWSKRD
                     YANAISLLVTLLERYPRDTLALKFMEWLFYCTGQAFNAEYFLKVCDKCAPENQDESHF
                     LAIHSFALELCGQYSKAKEMAEEAITMNLLTPWAHHTLAHVHLLTSDITGGINRLRDL
                     QKTWEDILPLLKGHNTWHLALFYLANRNEEEVKKLYPHISGALPDTVLEQLDTISLLW
                     RMDMAGLPQDRLLNQVVDHLSTHPLEYYTGFTNAHFIYCLVKSGYKNEADESLKRMKF
                     YACSPSSDALWGDVVLPLCQGIYAFADADYKTALMLMEPVIGECAQLGGSDAQIELFF
                     QTYLLVLIHNKQKDKALQFFTEHLKYYNNTPLSDWWFQSANKA"
     gene            complement(10001..12208)
                     /locus_tag="ACF2SP_000611"
     CDS             complement(10001..12208)
                     /locus_tag="ACF2SP_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFD2358417.1"
                     /translation="MEIFCFLIGVLYAYTYNIYLTIAIPLLFYITPRYPIVLFFIAGC
                     GFALIHQWIVSPKGMPDTQVITQAQLEGTIASIPMQHPNKTQFHFAIEKINNNPAQGL
                     IQLNWYNKPPVLRAGQRWQLSVKLKKPRNFHNPGGFNYVRYLAARHIHWTGYIRYRNN
                     KLISNQPPSFSWLALREKIGDALTKLAPDTETAGIIEALTLNVTHHISSDHWNLFRRT
                     GTIHLFGISGEHIALVSGIFFIVMRWIWSINYRCCLFLPANTIASIGGLLAALPYAFL
                     AGFEPPVQRALIGCVLYTIYAIGKQRFTPWQIWRYALFLVLCIEPHAVFLQGFYFSFL
                     AVACLLLTHQRWPLKNYKKTLALQLSCLIGLMPLTLHWYSYGSINGFLANLFAIPLVS
                     FLIVPLALMTMITSPFHWSWILMKPLAWLVALLLQGLTYVEQIAGINITWSITSVTMM
                     LAMTAGLFLRVLLPVKPFKHLAILWIILPLFPPRTAIYPGEALINILDVGQGLAVAIQ
                     TQHHVLLYDTGDSYFQGSDLGQMAIIPFFRSIGIKTIDKIVISHPDKDHKGGLNSLEK
                     EMKVNQLIVNDPYFYKRGSNCHSYPQWQWDGVSFRFLPIRESFNKKNNNSCILQVSNS
                     AGRVLLTGDIEKAAEEYLVRNYGSQLASEILVVPHHGSKTSSSYRFLLEVNPLYAIAS
                     LGFDNRFKFPHSKTLQSMKTLGITFFRTDECGMAAIKLPASGKINKPTCMQAV"
     gene            complement(12286..12735)
                     /locus_tag="ACF2SP_000612"
     CDS             complement(12286..12735)
                     /locus_tag="ACF2SP_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFD2358418.1"
                     /translation="MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQY
                     IQKSRRADAHATLTQDQIILERCYSQNFSYAAACGALPAFPQTTPNGYYTINISNLTA
                     TTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG"
     gene            complement(12747..16256)
                     /locus_tag="ACF2SP_000613"
     CDS             complement(12747..16256)
                     /locus_tag="ACF2SP_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFD2358419.1"
                     /translation="MDALKLCILIREGAQKAIRVLPRIFLAISLSASVSLYAVPLTIP
                     QIPLIIASPIHPQVLILIGNSQSMDGNLSGAIMTGSGSLSSGLSSLYNSSSPVNYTVP
                     AGFTPPVQGPDANGLAPYTVIQGGNKVDNSSSRLNVAKAGVKAIIENYMPTTDFALGT
                     YSTSNISTYNTWVYYMSPPGSNFVFTNTPVAGNRYVINPCYNYGSASSTVSSNCASIG
                     SLYGTSLVSSSQYLQIGGASDDPTINDVLYAGSGFPGVFVSFNGPSPATPYPPNFSLS
                     NYNNGNVLLSYSSTRPSIGSFATGPTNAGFVPFSQQVMYVQRGFGYYSNQSFNTGNMR
                     VNMTTAGTNPTTTSVTNAINAFLPHLRPETNSTSTTEIKAAAVQSPLAGLLTRSRSFM
                     KTVGTTSGNCPQKQYIILISDGLPTQDLQARYWPPLGSAAATGYGVTATFNADGSLNS
                     TNSQALSDAINEINALKNDGVLTFVIGMGAGVDPAVNPEAAATLRAMAVAGGTENYYP
                     ATSPQALVNSLNSILSNIQNGSFSTSAAAISSTRIDSSTVEYQANFITRDTPYQDWTG
                     NLTAIELDENTGQPTNIIQWSAQSTLDSLVSGTGWSTNRKIATWDPIAADGVPFRWAN
                     INATQQAQLQPSDLLGENRLEYLRGNATLEKRNGGTFRDRSHVLGDIIDSQVIYVGPP
                     SGPYLTASYISFAKANANRQPMLYVGANDGMLHAFNATTGEEFFAFIPNAVFNNLHQL
                     TSPLYNQNHLFFVNGSPESADVQFSDGSWHTILVGGENAGGKSIFALDITNPTNLSSE
                     PGVANAVLWEFADTDLGFTYSKPQIGQIRSGSPSSLNFAVFFGNGYNNTNNKSVFYAV
                     DPQTGTLLRKIDLCAAVPSACNVNLPQGLSTVALGQKDGLQADPVTVVYAGDLQGNLW
                     AIDVSSADPASWSARVLFQARDSAGTPQPITTPPLVTLNPNYPRNPGLFVVFGTGQLL
                     TTSDLVTTQRQTIYGVWDKPLSSVPYVRANLQQQTLTLVNSATSGLSTNIITATATTI
                     NWSNKVGWFADLPIAGQRLITTPELINGAFIATLNTPPLNSCGFGFTSMLLELNFLNG
                     GAFQYARFDISGDGGFDIADQYNGAYPVGIGLSNSYANSPTVLGPNKDNNLVILITQS
                     DGTQTTVIAPNITPRKIGWWQIQ"
     gene            complement(16269..16781)
                     /locus_tag="ACF2SP_000614"
     CDS             complement(16269..16781)
                     /locus_tag="ACF2SP_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFD2358420.1"
                     /translation="MNVSFTAQYSKQRGYVLITSLVLMSMLTVLALTQISQNTSQTRI
                     ATNATDSEISFEKTEGAINEAINLIINGTYNPQSFLASNNGMYLFDQSATTPLWKTIN
                     WSSGSAVINSFQGNTGAQAGYFLEKLPSVIMPGQSMKKPSNVYRITARSSGASGDTSV
                     LIQSTIQIQQ"
     gene            complement(16778..17845)
                     /locus_tag="ACF2SP_000615"
     CDS             complement(16778..17845)
                     /locus_tag="ACF2SP_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFD2358421.1"
                     /translation="MNIKYRLQQGFSLVELMIATLVGLILTYSILQIYLAQTQLYKAS
                     NSQDLIQSTENAISNLLIPMIRSAGFAGCGSIITAVSSLNAGGPRPLGILNTTPTILM
                     GYNGSGASFTITQSNPANSTNASHWSPALDSTLVGNAQQGSDVLVVLGSTSDSYPTGI
                     TAIDPGSNTFVVQGTSGMSLTSGMFGAVSDCGKTVIFQITGVAGTTITHDAGAGVLQN
                     ASSAFPVNFQTGSQFIPIQQTAFFVGQGQGGQSALMRASLVGNNWTIQPLVPGVEIMK
                     VQYGIGNNGVITRYVSANAVTDWAQVYAIRLGFLIAGQLGSGSLTTRQYNVLDTQVTV
                     PADNRLRHVFELTINLRNSIS"
     gene            complement(17842..18381)
                     /gene="pilV"
                     /locus_tag="ACF2SP_000616"
     CDS             complement(17842..18381)
                     /gene="pilV"
                     /locus_tag="ACF2SP_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFD2358422.1"
                     /translation="MINRMDTLAKDKGMTLLEVLISVVILAVGMLGIASMLIVSNKAN
                     NSSYAKLQAVQAVYDIFDRIRANSDAASNGSYNVSNIGSNGYPTSVSAPSVLCNASVC
                     NPNQLARYDTWYWLTRVVSQLPNGSGSITSALSGVAGNTVITVTVQWDDSKAQNELGA
                     SSASSGTANFVQFRVQSQI"
     gene            complement(18394..19101)
                     /locus_tag="ACF2SP_000617"
     CDS             complement(18394..19101)
                     /locus_tag="ACF2SP_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFD2358423.1"
                     /translation="MNKVITESQKFTLRNIANMLFCVESSVLPAQSNIAQKIQTQGIM
                     YNDLCKDSFDTEAPLNSNPLSIAGFTLVELLVTLSVFAIILTLIVPSLRTMILNSRLT
                     SNIDSLVSSLNYARGVALDRAVNVAVCPLGSPGSTACGANWSSGWIVVTQPVAGAPTL
                     LKSHQTSVNDPVITSNVSSVVFDPHGLSTTQSNFTMCDNRGNAFARSAMVLATGFVQS
                     GTTPGQAVWNNGALNCP"
     gene            complement(19323..20183)
                     /locus_tag="ACF2SP_000618"
     CDS             complement(19323..20183)
                     /locus_tag="ACF2SP_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFD2358424.1"
                     /translation="MKIKTVTRYLLLFSIFFLIGFNNVFAQKKAIAITIDDLPFVGEY
                     RNFHLNMMMNTMKDEKIPATGFIIAKEVRPDNWEILHKFREAGFGLGNHTFSHANLNK
                     LKAKEYIQEIKEADSLLMPVLTEPKYFRYPYLAMSSGSKKNKILCYLEKHHYQVAPIT
                     IDSKDFVFNQRLLSVPELGRRAYLDELKPFYLDFIWQQTLKAEEHTQYHHNPDQAQIL
                     LIHANLLNAYVLPDIINLYKQNGYTFVSLEDALKTFKENYHCHKTRILTKKPAGEETD
                     LNIESFMDWD"
     gene            complement(20360..21709)
                     /locus_tag="ACF2SP_000619"
     CDS             complement(20360..21709)
                     /locus_tag="ACF2SP_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0634 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358425.1"
                     /translation="METNMTFVLKEFDALKSHFNDTVKIILQREKKDKIEDLPNPRKE
                     ELQFLTAVLNQLEAKIDELKPRSLASYVHVFYGAMLLVCKDVENNLRVMEKKENSLLF
                     TRLMDGMGISDENIPTSEQNIMFYRGLNKFLNFIYESNDSRKGLKKEHFLQVLSLKKI
                     YSLAKLSYEQEEAAENNALAKLTADGKTKANANSFHVEKPIDSSIVEQFKSWDEMKGA
                     LHQLILDELSDKNVAKISALSQARSAQLKFLQTMAEQLDKIPNQSLEPSEKMAILAGA
                     MYIVRGQIAQEYGKDPLSNDKISATVIHTGLSTILHANADCCEDKEVLIAAANKFIRH
                     MVIERPEQSNKKITKESVRENNMFSDIAGFQLISVLTLIQNMIKTCRTDAIEACVTKR
                     KEELEALKPKKEGYSIASSVTGYVGSWFKKAPSMSEEDEEDDLKDQNTAEETSKPTV"
     gene            21997..23478
                     /locus_tag="ACF2SP_000620"
     CDS             21997..23478
                     /locus_tag="ACF2SP_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HFD2358426.1"
                     /translation="MSFQTDKEIQQKFFNYTLFVLVVLLICRLIANYFFPLFDTTESR
                     YAEIARKMLETGNWVTLQQDYGVPFWAKPPLSIWLSAFFMQLLGVNEFAVRLPGLLLS
                     IGVLWLVWGVAKKQSGSMAALFSLLTLAGSLYFFLDAGTVMTDPALLFCITLASVSFW
                     HAMVYENKIWSYAFFAGLGLGLLAKGPIAVVLVGMAVFFWVLLRNEWLNLWKRLPWVK
                     GTLLLLLIAVPWYVLAELRTPGFLNYFILGEHFHRFLTPGWSGDKYGIVHHVPKGTIW
                     LYATAGVFPWNIIAGGWLVKHGKMLPSLCQSGDGWLSYLTLWMITTLLFFTFASNIIY
                     PYVFPSLPAFALFFAEILNRLNLELKHLKWIFISSLLCGVLFLAVAFVFSVKPNVVPN
                     THKPIITAWLNQNPTAGSYLVYWGYKTDFSAEFYSEGQVKAVKNSNDLCKLISNRLEN
                     YLVINSKETQQIREGLLARFTLIDTFRVHRDTLILFYAPVLTC"
     gene            23669..24298
                     /locus_tag="ACF2SP_000621"
     CDS             23669..24298
                     /locus_tag="ACF2SP_000621"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFD2358427.1"
                     /translation="MAKLYFYYAAMNAGKSTVLLQSSYNYRERGMQTLLFTPAIDTRF
                     QYGTICSRIGLSEQAYAFNNSDNLYVLTQEFQLQTQKYSCVLIDEAQFLTREQVYQLT
                     EITDQMSIPVLAYGLRTDFRGELFPGSQFLLAWADELIELKTICHCGRKAIMNMRIDE
                     NGQAVIEGEQVLIGGNESYVATCRLHYKRGEAGKTFPRNKLFNKDTNTF"
     gene            24349..25617
                     /locus_tag="ACF2SP_000622"
     CDS             24349..25617
                     /locus_tag="ACF2SP_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358428.1"
                     /translation="MSHSVAVESRTFVPRGDFMAWVVCLSAGLFFLYEFFQLNIFDVI
                     NQSLREDFHIDATQLSWMSSTYLWADILFLLPAGLILDRFSTRKVILTAMLVCVVGTI
                     GFAVTESFFLASFFHFLSGIGNAFCFLSCVVLVSHWFPPRRQALVIGSLVTMAFIGGM
                     MAHTPFAYLNDLFGWRRALLIDGVVGAFLILWIYMIVQDRPEESPAHKLTNEGQILSS
                     FMKALSNKQNWLAGLYTSLLNLPIMVLCALWGASYLQVAHHLPDIAASNVVSLIFMGS
                     VVGCPLVGWLSDTQGRRKPLMIFGAIATLITTIPLFINVVLTQMSLSILFFALGLFTS
                     TQVISYPLVAESNQLENTGAATGIASVIIMGGGGVAQVLFGWLMTHHAGTNVTAYTVS
                     DFQFAMWMFPVAAIAGLVAVLMTRETYCKR"
     gene            25630..26913
                     /locus_tag="ACF2SP_000623"
     CDS             25630..26913
                     /locus_tag="ACF2SP_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358429.1"
                     /translation="MQVVDEFKAFDVKASLMPWLVCFAATMFFFYEFIQGNMFASIAA
                     NIMQDFQIQADKMAYLSSIYYLANVIFLFIAGMVLDRFSIKNTILIAMFLCVISTFIL
                     SYSDSFYVALFCRFVTGIGSAFCFLGPVRLASHWFPPQRMALVTGAIVTVAMTGGMLA
                     QYPLTKLVLYVGWRQAVQDVGILGLAMLVLMFFWIKERPQVAIKKEGKPINVLVAARK
                     AYLNAQNLRAALYTSLMNMAIAVFGAMMGTLYLEQRLGISPDQAAMINGMLFFGAIIG
                     SPLMGWISDKVGLRVLPMKGGVLASLLILLGVLYLPVSVTMMAVLFFLLGLFTSAQVI
                     SYALVAESSPPMMTATAVSVVSILTQGGYLIYQNLFSALLTNYGDVRLINGVPVYLLE
                     AYQHAAIILPIGLLIALLLLFGLKETRCQQVEHTV"
     gene            26910..28133
                     /locus_tag="ACF2SP_000624"
     CDS             26910..28133
                     /locus_tag="ACF2SP_000624"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFD2358430.1"
                     /translation="MTGRVCVLVMDSFGIGASLDAARYGDAGANTLVHIYEACKRGEC
                     DIDGVRKGPLMLPNLAGKGLYHAAMASSGLPFIDLSALAIPSGYYGYAVEQSLGKDTP
                     SGHWEMAGVPVTFEWGYFPDKSYCFPEELISEFIKQCNLPGVLGEKHASGTIIIDELG
                     EEHIRTGKPIVYTSADSVFQIAAHEEAFGLQRLYDICKIARNLVDKYQIGRVIARPFT
                     GKPGSFKRTGNRKDYATPPPEKTLLDFLKEDGREVIAIGKIADIYAHQGVTQEIKADG
                     NMALFDATLSAMKTAPQGSLVFTNFVDFDSSYGHRRDVAGYAHALEQFDVRLPELEAL
                     LQPDDMVFIAADHGCDPTFPGSDHTREHIPVLMFGPQVNSKFIGRRDCFADIGQSIAE
                     HLQLSSPLTHGVSFL"
     gene            28337..28885
                     /gene="hslV"
                     /locus_tag="ACF2SP_000625"
     CDS             28337..28885
                     /gene="hslV"
                     /locus_tag="ACF2SP_000625"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFD2358431.1"
                     /translation="MEQFHGTTILSVRRGNQVVIGGDGQVTLGNTVMKGNARKVRRLY
                     KDKVIAGFAGGTADAFTLFERFEAKLEMHQGHLIRAAVELAKDWRTDRILRRLEAVLA
                     VADSKASLIITGNGDVIEPEESLIAIGSGGPFAQAAARALMENTQLSAKEIVQKALTI
                     AGDICIYTNNNLTIEELNDEGK"
     gene            28887..30212
                     /gene="hslU"
                     /locus_tag="ACF2SP_000626"
     CDS             28887..30212
                     /gene="hslU"
                     /locus_tag="ACF2SP_000626"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFD2358432.1"
                     /translation="MVMTPREIVQELDKHIIGQDDAKRAVAIALRNRWRRMKIKDPVL
                     RNEIMPKNILMIGPTGVGKTEIARRLANLAKAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LTDMAIKQEREFAMKKVEHLAEDAAEERILDVLLPPARGTLTPGEKNTTARQVFRKQL
                     REGELNDNEIEIEVAATPVGIEIMAPPGMEEMTSQLQSMFQQVGSYRTKTRKMTVAKA
                     MKILREEEAAKLINEEDIKLKAIESVEQNGIVFIDELDKIAKRSDTVSGGDVSREGVQ
                     RDLLPLVEGTTVSTKYGMVKSDHILFIASGAFHVAKPSDLIAELQGRLPIRVELSALS
                     VEDFVRILTEPSASLTLQYSALMETEGLTLTFDETGIRRIAEVAWQVNERTENIGARR
                     LYTVMERLLEVVSFEATDKAGETVHVDKAYVDKNLGQLIADEDLARYIL"
     gene            30412..31986
                     /gene="wipB"
                     /locus_tag="ACF2SP_000627"
                     /pseudo
     CDS             30412..31986
                     /gene="wipB"
                     /locus_tag="ACF2SP_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946379.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipB"
     gene            complement(32136..33374)
                     /locus_tag="ACF2SP_000628"
     CDS             complement(32136..33374)
                     /locus_tag="ACF2SP_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFD2358433.1"
                     /translation="MNWKEKVKTKFYSCDRFVRFVIQHFIQDDCTYIASALAFTSLLA
                     VVPLMSVGLAIFSSFPVFQGLAEPVQNFIFDNFVPATGKIVQSYLQQFTSQVSKLSIW
                     GIVFLIFTALLVMFTIEQAMNKIWRVSSSRHGVSAFLLYWAIISLAPVLLGLSLAASS
                     YLFSMPILADHRAPYTILHYSPFFLSLIGFTFLYVVVPNCPVKIRHAFWGGLVAAILF
                     ESAKHAFAYYLIRYNTYELLYGAFAAVPIFFIWVYWVWIITLLGAEISYAFSVHHQRR
                     GGKSLDGFSHALLWLHQLWLAQQHGKGLSFNDLVDASKQPFAVDVDEMINALIYHELI
                     HATADGHYMLSRDLSHVTLYDLTQLLPYRLPTHLELQYSKASLAEQWRAAFKRHNEEL
                     KKSLDINLEELFKKTGTVLK"
BASE COUNT         9784 a         6067 c         7087 g        10472 t
ORIGIN      
        1 cgctgttcga ttggtccatc gctgaaatcc atagattaaa gaataaaaac ttggttttgg
       61 agtgtatttt gattgacagg acttacgaaa accccaaagt caaggcaaaa aatgttttta
      121 atgagggggt ttagataaac taaatgaccg aattaaaaca ttttttaaca tagagattgg
      181 gttttttcgt aagtcctgat tgatagaaat tatgcattag ggaggattca ttaaatatga
      241 agaaatatca tcaattactt atgggaatta ctttactgtt gtttggtgta tgtaattatg
      301 ccggtgatac agggccagtt gaaaatagcg accttttccg agtgttcatt cctaatttga
      361 aaccgggata tgacttttct gcgacagcct tttatctgca accgggagcg aataatttag
      421 gttggggggt gattacgaca gtactgccaa taccatcgcc taattggcag gttgttactt
      481 ttaatccaga ttatcaagtt gggtttaatt tgggaggacg ttatgtattt gccaattctg
      541 gaagcgattt gcaattaaac tggtctcatc tgcgtaccaa tgatactgat aatgtctttg
      601 tgaatccctc atcgcaatgg atctctcctt tttctcagac cggtactcct cctacgcctt
      661 ctggccaaat tacaggagtg gctttattga agcttgcaca tgccagtctt gattttagtt
      721 atgatgcagt cagtctcgat gttggaaaat ttattaactt tggttccaaa ttacagacaa
      781 ggctttttac agggttaagc agtgcctgga taaaggaaga gttgatatca acttttcggg
      841 gcttttcact ccctatactg tcattaaata atacatccac ttacaaaggt gttgggccga
      901 gactaggatt caataatagt tataatattt ctcatgggtt caatttggtc ggacaactcg
      961 ccggctcagt cttgtacggc agaatgcaac cagctcaata ccaattcaca ggaacctctc
     1021 agcaattgat tatcgctgga atctttgtaa atcgtgaagg acttgctaat cccagtgtca
     1081 atcaactggt tccagctctg gatgccaaaa ttgggttaag ttatctttac tcattgaaac
     1141 aaaattggga tttgaatttt gaagcagggt atatgggagc tttgtatttt aatcctttat
     1201 ccagttatga aacaaatacc aatgttattg ccttggacac tggctctttg tcaacatcaa
     1261 gtgccaaaca cacacaaagt aatttttctg taggtggccc ttacattaca gtcagcttga
     1321 gaacctgatt agggtggtca aactatcatt ccctggccta acaaaatatg aaaaccgaat
     1381 ttctaattta tggttaggcc aggacacgac agataatccg gtttaaatga tttcgctaaa
     1441 aagcttatgg agaatatgag tcaccttcag gagttcgagt gatttcttta tttggctcga
     1501 aaacaggtta cgatgtgatc attcaccata ccaatggctt gcatgtaagc gtagatgatt
     1561 gttgagccaa caaagctcat tcctcttttt ttcagatctt tggataaggc atctgattct
     1621 ttgctggttg ctggtatcgc tcgtatgctt tcaggacgat ttatgatagg tgctccatta
     1681 acaaattgcc aaacgtaaga atcaaacgat ccatattctt gttgaataga tagaaaaacc
     1741 ttcgcatttt ttctggtaga aaagatttta agccggttgc ggataatttc aggattagtt
     1801 aacaaagcat tcaattcatc atcagtcatt tgggctacgg cttgtgggtt aaattgcttg
     1861 aaagctctcc ggtatccgtc gcgctttttt aatatagttt cccaatttaa ccctgcctga
     1921 gcaccttcca gaatgagcat ctcgaaatgt ttttcatcat catgaacagg aattccccat
     1981 tcagtatcat ggtatagttc ataatggggt ttgttttgcc ctacccattc gcatcggttt
     2041 agtatgatgt tttgactcat aatgtaatta ttagattggg ttaaaaatat ttaatcctgg
     2101 taatggcagt atttataaca ttggtctact attctgtgtg aactattcat taagttgtat
     2161 tcgttgtcaa gtaatgaagg taatcaggaa atcgcttgtt aacagtataa ggacttacga
     2221 aaaccccaat ctctttgtta aaaaagtttt taattcggtc atttagttta tctagatccc
     2281 ctcattaaaa atattttttg ccttgactgt ggtgttttgc ataaatccga ataccaatca
     2341 agttgttcta aaaaaaacaa attaaaggag attcttatga ctcaagctgc gtttggagag
     2401 ttttgttgga atgaattagc cacttctgat gttaaaaagg caaaggattt ttatggcaag
     2461 gtctttggtt ggcaatttag agagattgaa tcaaaggaaa tgacctacac tcttattcaa
     2521 tgcaatgata aggaaatggc tggtatatgg caaattccca aagatcaaca aagtcatatt
     2581 cctcctcact ggatggctta tatttatgtc caagatgtat cagaggcttt aaaaaaagca
     2641 aaacaacatg gtgctcagga agtcaaagga ataacgcagg ctggtgaaat gggacgtttt
     2701 gctattatag tcgatccgac tggtgcacat gttgccttat gggagtcctt tgaataatac
     2761 aaaatagaca ggtgacgtat gaagtctaat ttagtttata gttaattcct gaagattaaa
     2821 aaattgtaaa agctattggg tttataaggg aatgctttta taaaaaggga ttactgttga
     2881 tttcctgagc tcggtcattt tttggaggtt tgtcaggagc ctctgtcagg attttattca
     2941 atcccccgtg tttattaata atctccccca agcgaccatc ggaaagcgcc tttctttctg
     3001 atggatttag attacatcgt tcatggcatt ggggatcaag aattttctgt aatttgcttg
     3061 ctgcagttaa tttgacctca cgggctttat gattgaaatg atcagttcca agaattaaat
     3121 cctgtatgaa ataaacgaaa gccactaggc ctagaaaatt ataatgataa ctccattcat
     3181 ttcccctttt gtttatatat tgagcaagat ccttaaggat aggatgagca gtattcggtt
     3241 tggaccgttt gggtgaaaag atagtcactt gtggaggttg acttgatttc tctgttgtct
     3301 ttttcacagg ggaatttgat tgatgtttga ccaataactc agtttgttga catggagaat
     3361 aaccctgtgg agcaatttgt ttaaaaactc tattacaagt ctcataaagc tcatgatact
     3421 tgttttgaga atccgtatct tttgaactca ggtaattgga agtggcttgg aacaaggtca
     3481 aacttgagat gaatttacga tcatcaggaa aaccacccat cagatgaggt agaggaatgt
     3541 tctttaattt ttcctctatg agttttatta actcaaaata gcgcttaaac agtacaggat
     3601 cttttaaata atcacatgcc tcattaaaat ctttaattcc atatagtttg gccgatgggc
     3661 ttttacctaa attttccaat tgaggaaaaa tataccatat ccaatggctt ctttttcttc
     3721 cgtcttgtat ttcgctgaat gcttgatcgt agccacaagt attactattt tgagcattat
     3781 gaaaacgatc gagtctttta tttctggcca tgaatttttc tcttgatttc attaattttt
     3841 atattcacaa atcaggtatt acgcataaat tgactattta taaatcaatt ataacaaagt
     3901 tatgaaaagt tgttatattt gttaacgagt tggtttggat atggaatcaa aagttgtagg
     3961 tacttgttca ttagttgtat cagtggtttt ctgttctgct atttttgtgt gctctcgaga
     4021 agatgagcca aataaagtaa atctggaatc taatgagctt acagtacttt cagttggcga
     4081 caaagcaggc actttatcgt gaactattaa tatatttttc acctgtctcg cagggtttga
     4141 aggactgact aactcaagat ttaattgttg ctcataaacg tttaatgctt catctaaaac
     4201 tgcatcctcg tgtttcgcag ttttttctgc ttttaattga gcgtcctcta tttcagccat
     4261 tttttcagcg gtccacttga ttaaaccaat gattgccata acactaccta ctgcaaacat
     4321 gatagggacc ataatcggag gtgcggcaat cacgccagct accaccaata agccggctat
     4381 cgatattaaa gtactggccg ctgctatagc aaaggtactg taacttaatg gcaaattaat
     4441 ggtagatttc aaattgtctc gttctatttt agcaagtggg gctatgattt cttctatgct
     4501 atcgtcggtt ttggttattt ctttttgtaa tgctttgatt tcgcttaaaa gtttgttttg
     4561 ctcagctctt tttccagttg gattgattaa agcaatttta tctacaagag tttttaattg
     4621 ctcagcgcgt ttagcataga gttggtttag tttgtaagcg tcattctctt tgttgttgcc
     4681 cggggtgata ttttttttcc taagtaccgc attaatgaaa ttcaactctt cttttaattg
     4741 ttgctgttca atttgattgg gataagattt ctcaagttca tgttggagtt ctatggcacg
     4801 aatttcgaat aagtcattat atttatcatt gtttggctct atacgtttcg ctacaagatc
     4861 tataaattct tttttcattt tgtaggaatt tgccaattga aatttttcag caattttccc
     4921 aaaaaatccc aatccttcca tgatggttac agtagaagca attactgtcc cgatgattgc
     4981 ggcagccaat ccaccgaggg tgatcgctgc aatagacagg gcgataatag cagcaattag
     5041 aatcgaagat attatttttt cagcaataga tttttccgga tcgctcacaa ttgagattga
     5101 tttagctaat atggccacca attgcaaaac cccacccacc agtggaattt gtcggtagga
     5161 gcttaaaaag gaagcaataa cattaaacaa gtgatttatt cccgttgtta ttgttgatgc
     5221 tgtggtagtt ccagcatgaa tttgctcgcc taacgatttg gattgtgtca gttgctcctg
     5281 aagattcttt ttaagagttt tcatcttgtc tgacaatcgg atgggagttc gatatttttt
     5341 ggacataaaa agaaccctaa aggcatatat aaaactatat gagttatttt agtacataat
     5401 tttacttgtt aattttcctt ttgggaagat atttgtttgg aatttaaaca aaaaatcaac
     5461 aaagaagagc ttggcacact atttttattg acgcatcctt gttgttttaa ttattgctgg
     5521 caaaagtttt ataatgaata gtatatttcg tttgccagtg cgacgaaggt acttgctttg
     5581 agttaatgga aaatgaaata acctttttac ctgccaggta attattctgg ctaaataata
     5641 gtccaaactc agcgctgttt tctatgaatg gggataatag aaaaccggat tttgccatat
     5701 tggaaatgag tctatattgt ctttgggtgc cattttttaa ttccaaattg gcttcaagct
     5761 ggtgaggctt gaaaaaggta gtcgctaaca tcccccagag cgttggttga atatctattt
     5821 cgacaaaaag agatttatct gtatcaggta ttttaacttg ttctccaaaa gtatgaattt
     5881 ctgtttttaa tggcactaaa gatgttacag gtttgttgac taattttttt cttaaaaata
     5941 aaaaatcatt ttctaaccgt actggttggt aatgattaat cagtatcggc caactcattc
     6001 cgtcttccat tgaaggaatt ctattgtcaa ttggttctac tttaaaaagg atgttatccg
     6061 gtttgttctc acttaaaagg taattcgtat tgatttctgc catttttgca gtaaaaacgg
     6121 aataactctg aaagattggc cttggcgacc atgtcattcc cgaggcgatt aatgaagttt
     6181 gattgtaaga ataaatatcg gtagtacccg gtaatctggg aaaatcatcg cgggctttca
     6241 aataattcat cgttaatgaa aaattttctt ttaaccaatt cttattctgg attctgtttt
     6301 taaggccata gtaagcggcg gtataggtgg aaatgaaatt atctcgaaca gatattttgg
     6361 tatagtgccc gttgatataa ctcgctgtat aaagggataa agctaataat gggactatta
     6421 ctttgttgtt ggaattaaaa tacggtaaca aaaaagaggc tagcaaaaca gaggtaccag
     6481 caatgaaggc atggccaaaa tgtcttgtga aacccgtttt atatgagagg aaaagaaaaa
     6541 cgagatagat acaaaataga aatgctttgg ataatggtga gagctgtttt tgcaggagta
     6601 ttgataagag aatgcaagaa gaggtaaccc caaaaaatat cagttcttta ttgtctcctt
     6661 caattgccat ggcttcagta aaacctgatg cgagcaagat agtgttactg acataagctg
     6721 gcagattgaa aatagattgc ccggatgcta tccaaaaaaa taatatggaa actactggag
     6781 atgccagaca aagcacagca agatttcgct gtttatatag gatcaaaaat aaagagcata
     6841 agagcacggt aataatactt aatattaaaa gagagccctt tatcagagga aataaaccga
     6901 atggcgtaaa aagcaaagca aataaagtgc agaaattgct tttaaaggat gtttcttttc
     6961 cagaaatttc tttatgaatg agtaagccaa tgattaatga ataggaaaaa aacaaagaat
     7021 cccgagcata gatcatacta aagagaagta ttgcaaaggc aatactccaa cgccatttaa
     7081 tgccttgcat taaaaggata atagctatcc agtaacataa agccaaatac aaacttcccc
     7141 caaccatcat gaaatcggta gctggatggt agaattttgt atagattgaa gaataaggtc
     7201 ctaaggtaaa aaaaatgtct ttaccaaaag ccaaaccttg ggcaactgct tgattaagtc
     7261 ctacagccca agaggcatcg agaccgggag ctggcatttt gggggatagt ggaataaaaa
     7321 cggcgattat aataatgagc aaagcagatt ttaataacca ccccaaagtg agtggaatta
     7381 ttttaagatt tgaaataacg gtcgtttgat gagggcaggt ttgggctcta gtcatttttt
     7441 ttgtaggcat atcatatcaa gcatcatatc atagcattcc ttgctgctga attgacaagg
     7501 actttatcgc ttcctttccc catcaacaga atgagtcata gtacctcaat aactcttcct
     7561 ttatttcaaa taaacataat agacattaaa taacaggaac aaaccaataa taaagaatat
     7621 tacagctgct atttttagtc tcattggatt atcgacaaat gattggccta ttctaactgc
     7681 ggtatcgggg cataaaaacc taattaaacc accaagtaag actaaccatg caaaaagagt
     7741 aataacaaca ggccagccca ttacccagat attatgactg acgactaaaa gcaaacctgt
     7801 aattaaggtt actatcgcca taatcaattg cgatccgcga tgagctataa tctctgccat
     7861 agctgatttt gcatgctcat tatggaataa taagaataag cttactatga ctaaatacca
     7921 gccaatcact gtggctagaa atgtagttaa catcttcaaa ctccttacaa tgacctccta
     7981 ctataattat agcaattttg tgagctatgt gtcggaaagt taaaggaaaa ttgaggagta
     8041 agcgagatga cacactgtta tttgatttga aaatggagag taatctaaca tgatgaaaaa
     8101 aattaattta ttcaaattgc ttatgatctt tgttctgggt acaagtgcgc tccaggctgc
     8161 aggattatca actcaacgta ttgagcaatt atctaatgcc agggtgactg tgtggaaaac
     8221 aattatctat ccctcttcaa aacaagtttt gcccatgcac aggcatgata acgatcgagt
     8281 tttggtcgca ttgacggatg ggacattgaa aattacaaac aataaaggca aagtacacta
     8341 tttaaaatta aagaaaggta agtcatatta tctcaccaaa gacgccccta acgaattgca
     8401 taacgatgaa aacgtaacta accatccaat tcaggtgatg gtaattcaac tcaatgataa
     8461 agcatgatta gcaggaagtg ggtaatctgg tgttgtatta atttctggtc agggaaaaca
     8521 attttagcga gagtacttgg agctctaccg ctgtcatcgt gatatcaggc cgtactatca
     8581 tctaattgga gcgttgcttc cgtccttgtt gtttattatt tgtgctatca tttaatcatt
     8641 gattcattca ttgacaggat ttcgagaatg catactcaac gtggattgga agtaacgaca
     8701 ggctccatag aggcagtaga tagtatcaat tattttcacg aacaggtact aagctccggt
     8761 caaaacgcaa tccaaattct ggattcagca aaaaaacata ctgacaattt attaattcaa
     8821 acttatgcgg cggcttttta tttgtatgca caagaagatg tagcgaatga acaagccagc
     8881 gattatttgc aatcggcatt caggttatta acatcagcga atgaacgtga aaaattaatt
     8941 tttgaagcag taacgaattg gtctaagcga gattatgcca atgccattag cttgttagtt
     9001 actctgcttg aaaggtatcc cagggatact ctggctctga aattcatgga atggcttttt
     9061 tactgtacag gacaggcttt taatgctgaa tattttctta aagtatgtga taagtgtgct
     9121 cccgagaatc aggatgagtc ccatttttta gccatacatt cctttgcttt ggaattgtgt
     9181 gggcaatatt caaaagccaa ggaaatggct gaggaagcta tcacaatgaa tttgcttact
     9241 ccttgggcac atcataccct ggctcatgtg catttattaa caagtgatat tactggtggt
     9301 atcaatagac ttcgagattt acaaaaaacc tgggaggata ttttgccttt gttgaaaggc
     9361 cataatacct ggcatttagc gctattttat ttggcaaatc gtaatgaaga ggaagttaaa
     9421 aagttatacc cccatatttc aggggcattg cctgatactg tgcttgagca gctggatact
     9481 atttccctgc tatggcgcat ggatatggca ggtctgcccc aagacaggtt gttaaatcag
     9541 gttgtggacc atttaagtac ccatccattg gaatattata ccgggtttac gaatgctcat
     9601 tttatttatt gtctggtgaa gtcaggttat aaaaacgaag cggatgagtc gttgaaaaga
     9661 atgaagtttt acgcctgttc accttcttct gatgctctat ggggcgatgt cgttttgcct
     9721 ttgtgtcagg gtatctatgc ctttgccgat gcagattaca agactgcctt gatgttgatg
     9781 gagccggtga ttggtgagtg tgcacaatta ggtggaagcg atgcgcagat agagcttttt
     9841 tttcagactt acttgttggt gttgatacac aataaacaaa aggacaaagc gttacaattt
     9901 tttacggaac atttaaagta ttacaataat acgccattga gtgactggtg gttccaatcg
     9961 gctaataagg cttagatata acatgggtca gccaatccat ttatacagcc tgcatacagg
    10021 ttggtttgtt aattttacct gacgcaggta gtttgattgc tgccattccg cattcgtctg
    10081 ttctaaaaaa agtgatacct aatgttttca tgctttgcaa agttttagaa tgtggaaatt
    10141 tgaagcgatt atcaaatcct aaggatgcaa tggcataaag cgggtttact tccagcaaaa
    10201 atcgatagga tgaagacgtt ttgctcccat gatgaggcac aactaaaatt tcggatgcca
    10261 gctgactgcc gtagtttctt accaggtact cttcagctgc tttttcaata tctcccgtta
    10321 acaaaaccct gccagccgaa ttacttacct gtaagataca agaattgtta ttttttttat
    10381 tgaaagattc cctgatgggt aaaaaacgaa aactcacccc atcccattgc cattgcgggt
    10441 atgaatgaca attacttcca cgtttataaa aatagggatc attcactatt aattgattga
    10501 ctttcatctc tttttcaagg gaatttaatc cgcctttatg atctttatcg ggatggctta
    10561 ttactatttt gtcaattgtt tttattccaa ttgacctgaa aaaggggatt atcgccattt
    10621 gccctaaatc actcccctga aagtaggaat ctcccgtgtc gtagagtaaa acatgatgtt
    10681 gcgtttgtat cgcaacggca agtccttgcc caacatccaa aatattaatt aatgcttccc
    10741 cgggataaat tgcagtacga ggtggaaata agggaaggat aatccaaagg atagccaagt
    10801 gtttgaatgg cttgacaggt aataataccc ttaaaaacag acctgctgtc atagcgagca
    10861 tcattgttac cgaggtgatg gaccaggtga tattgatccc tgctatttgc tcaacatacg
    10921 ttagtccttg cagtaacagg gcaacaagcc acgccaatgg tttcatcaat atccaggacc
    10981 aatgaaatgg actcgttatc atggtcatta aagccaaggg tactatcaga aaactgacta
    11041 aaggtatggc gaataaattg gcaagaaatc cattgataga accataggaa taccaatgga
    11101 gagtcaaggg cattaatcca atgaggcagc ttaattgtaa tgccagtgtt tttttataat
    11161 ttttcagtgg ccagcgttga tgagtcaata gaagacaggc cacggcaaga aatgaaaagt
    11221 aaaaaccttg caggaatacg gcatggggct caatacacaa gactaaaaat aaggcatatc
    11281 tccatatttg ccatggtgta aaacgttgtt ttcctatggc gtatattgtg taaagtacac
    11341 aaccaattaa agcacgttgc acaggaggct caaacccggc taaaaaagca tagggcaagg
    11401 cggccagtag cccaccgata ctcgctatgg tattggccgg taagaacaaa cagcatcgat
    11461 aatttattga ccaaatccat ctcattacta taaagaaaat gccagagacc agagcgatgt
    11521 gctctcctga aatcccgaat aaatgtatcg ttcctgttct tcggaacaaa ttccaatgat
    11581 ctgaactgat atggtgagta acatttaacg tcaatgcttc gatgatgcct gccgtttctg
    11641 tatccggcgc cagttttgtc aaagcatccc ctattttttc acgtagtgct aaccaactga
    11701 aagaaggggg ttgattgcta attagcttat tattccgata acgaatatag ccagtccaat
    11761 ggatatgacg agccgccagg taacgtacat aattaaaacc tcccgggttg tggaagttac
    11821 gaggtttttt tagtttcact gaaagttgcc atcgctgacc ggctcgtaag acaggtggtt
    11881 tgttatacca gtttaattgg atcaagcctt gagcagggtt attattaatt ttttcaattg
    11941 caaaatggaa ttgggttttg ttaggatgtt gcatcgggat ggaagcaatt gttccttcca
    12001 attgggcttg tgtgattact tgcgtatcag gcatgccctt gggagataca atccactgat
    12061 gaatcagggc aaagccacac ccggcaatga agaacaggac aatgggatat cttggtgtga
    12121 tataaaagag taaaggaatc gcaatagtta gataaatatt ataggtatag gcataaagta
    12181 cgcctattaa aaagcaaaaa atttccattt caaattaagt tggactttcc cttactggca
    12241 aatctactcg aattgaatcc tttgtgtaaa gaatttttta cagtattaac cgggattcca
    12301 gcattctggt tgcgcattag cggaggaatc tactgctgtt tttacattgg cctggttaat
    12361 tgacatgctg gcacactcgg tatctttggc ttgagttcca acaggggttg cagttaaggt
    12421 atacgttgtg gctgtcaggt ttgaaatatt gatagtatag tacccgttcg gcgttgtttg
    12481 aggaaatgct ggtaaggcgc cacacgccgc agcataagaa aaattctgtg aataacagcg
    12541 ttctaaaata atttgatctt gtgtcaatgt ggcgtgagca tcggcacgac gggatttttg
    12601 gatatattgt aaataggatg gataggcaat tgaaaccaga atgcccataa tgaccatgct
    12661 gatcagaact tcaaccaggg taaacgcgga ttgtttcata cggctattct tcataaaatg
    12721 tacatgactg agcatttggg tatcctctat tgaatttgcc accagcctat ttttcttggc
    12781 gtaatgttag gagcgataac agtagtttgt gttccatccg attgtgtgat cagaatgacc
    12841 aaattgttat ccttgttagg ccccagaaca gttggtgagt tggcataact gttggacaag
    12901 ccaattccaa cggggtaggc cccattatac tgatcggcta tatcaaagcc tccatcgccg
    12961 gaaatatcaa atcgcgcata ttgaaaagca ccgccattta agaaattaag ctccagaagc
    13021 atggatgtga aaccaaaacc gcacgaattg agtggaggag tgtttagcgt tgcaataaat
    13081 gctccgttaa ttagttcagg tgtagtaatg agtctttgcc ccgctatagg cagatcagca
    13141 aaccatccca ctttgttact ccaattgatg gtagtagcag tggcagtgat aatgtttgtg
    13201 gataaacctg atgtagccga gttaaccaag gtcaaagttt gttgctggag attggcacgt
    13261 acataaggca cagatgaaag cggcttgtcc catactccat aaatggtttg tctttgagta
    13321 gtgaccaaat cggatgtggt cagaagttgt ccggtgccaa acacaacaaa aagtcctgga
    13381 ttgcgaggat aattaggatt caatgtcact aaaggaggag tagtgatagg ttgcggtgta
    13441 ccagcagagt ctcttgcctg gaataataca cgcgcactcc aactggcagg atcagccgag
    13501 gaaacatcaa ttgcccacag attgccttgc aaatcaccgg catacactac agtaacagga
    13561 tcagcttgca atccgtcttt ttgtcccaaa gcgactgtcg ataatccctg gggtaaattg
    13621 acattgcagg ctgaaggtac agcagcacaa agatctattt ttcttagtag tgttcctgtt
    13681 tgagggtcta cggcataaaa taccgatttg ttgttagtat tgttgtatcc attgccaaaa
    13741 aagacggcaa aatttaatga agatggactt ccggatctga tttgacctat ttgaggcttg
    13801 ctataagtga accctaagtc agtatctgca aattcccaga gcacagcatt agccactcct
    13861 ggttcagaac tcaggtttgt cggatttgta atgtccaggg caaagatgct tttaccgcct
    13921 gcattttcac ctcccaccag aatggtgtgc caggaaccat cggaaaattg tacatcagcg
    13981 ctttccggag agccgttgac aaagaataaa tgattttggt tgtacagggg agaagtgagt
    14041 tggtggagat tattaaacac ggcattggga atgaatgcaa aaaactcttc tccagtggtt
    14101 gcattaaagg cgtgaagcat tccgtcattt gccccaacgt ataacatggg ttgtcgattg
    14161 gcattggctt ttgcgaagct gatgtaactg gcggttaaat aggggccgct tggtgggcca
    14221 acataaatta cctggctatc gataatgtct cccaatacat gcgagcgatc acggaatgtt
    14281 cccccgtttc ttttttccag agtggcattg ccacgcaggt attctaatcg gttttctccc
    14341 aacagatcac ttggttgcag ttgagcctgt tgggtggcat taatattcgc ccagcgaaac
    14401 ggtactccgt ctgcggctat aggatcccaa gtcgctatct ttcggttagt tgaccatccg
    14461 gttcctgata ccagggaatc aagtgtcgat tgagctgacc attgtataat atttgttggt
    14521 tggccagtat tctcatccag ttcaatagct gtcaaattac cagtccaatc ctgatagggt
    14581 gtatctctgg ttataaagtt agcttgatat tctacagtac tactgtcaat acgagttgag
    14641 ctgatagctg ctgcagaggt agaaaaagat ccattttgaa tatttgagag aatggagttc
    14701 aaactattga ctagagcttg aggacttgtt gcagggtagt agttttcggt acctcctgca
    14761 acagccatgg ctctaagggt tgcagctgcc tctggattta cagcaggatc tacgccagct
    14821 cccatgccta taacaaaagt aagaacgcca tcgtttttta aagcgtttat ttcattgatt
    14881 gcatccgata aggcttgact gttagtacta ttcagtgagc catcggcatt aaatgttgcg
    14941 gtgacgccat agccggtcgc agccgcactt cctagtggcg gccaatatct tgcctggagg
    15001 tcttgagtgg gtagaccatc agaaattaat ataatgtatt gtttttgcgg acaattgccg
    15061 ctggttgtac cgacagtctt catgaaactt cgagatcgtg tcaataatcc tgcaagaggg
    15121 gattgtacag cggctgcttt gatttctgtc gtcgatgtag aatttgtttc aggtcgaaga
    15181 tgaggcaaaa acgcattaat tgcattagta acactagtag ttgtaggatt tgttcctgcc
    15241 gtagtcatgt tcactctcat attgccggta ttaaaagatt gattgctgta atagccaaaa
    15301 ccacgttgca catacattac ctgttgtgaa aagggtacaa aacctgcatt tgtggggcct
    15361 gtagcaaaac ttccaatcga gggtctggtt gaagaatagg aaagaagtac attaccatta
    15421 ttataattgc tgagagaaaa attcggagga taaggtgttg ctggagatgg gccattaaaa
    15481 ctgacaaaaa ctccaggaaa accacttcca gcatatagaa cgtcattaat agttggatca
    15541 tcgcttgccc ccccgatttg tagatactgg cttgaagaaa ctaaactagt accatacaag
    15601 cttccaatgg atgcacagtt acttgaaaca gttgatgaag ccgaaccata gttatagcaa
    15661 gggttgataa cgtatcgatt tccagctacc ggggtgttgg tgaacacaaa attacttcca
    15721 ggcggcgaca tgtaataaac ccaagtgtta taagtactga tgttacttgt gctgtaggtg
    15781 cccaatgcaa agtctgttgt aggcatgtaa ttttcaataa tcgctttgac tccagctttg
    15841 gccacattga gtcggcttga actgttgtct actttattac ctccctgaat aacagtataa
    15901 ggagctaagc cgttggcatc agggccttgt actggcggag taaatccagc cggtacggta
    15961 taattcacag gtgaactgga attgtaaagg gagcttaaac cggaggacag agaaccagag
    16021 cccgtcatga ttgctccact caaattacca tccatggatt gagagtttcc tatgagtatc
    16081 agaacctgag ggtgaattgg actggcaatg attagaggta tttgtggaat ggttaaggga
    16141 acggcataaa gagaaactga ggcagacaat gaaatggcta gaaatattcg aggtagaact
    16201 ctaattgctt tctgagctcc ttctctgatt aaaatgcaaa gttttaacgc gtccatgata
    16261 atcaaaacct actgttgaat ttgaattgta ctttgtatga gtactgaagt atccccgctt
    16321 gctcctgatg accgagcagt tattctgtag acattacttg gctttttcat actctggcca
    16381 ggcataatca cggacggcag tttttcaagg aaatagccgg cttgtgcccc tgtattgcct
    16441 tgaaagctgt taatgactgc cgaaccactt gaccagttaa tcgttttcca aagtggggta
    16501 gtagcacttt ggtcaaataa atacattcca ttattagaag caagaaaact ttgagggtta
    16561 taagtgccat ttatgatcag attaatggct tcattaatgg cgccttcagt tttttcaaaa
    16621 ctgatttccg aatcggttgc gttagttgca atcctggttt gagatgtgtt ttgtgatatc
    16681 tgggtcaggg ctaaaacggt aagcattgac ataagcacaa gagaggttat caggacgtaa
    16741 cctcgctgtt ttgaatattg agctgtgaaa gatacgttca tgatatcgaa ttcctcaagt
    16801 tgatggttaa ttcaaataca tgtcttaagc ggttatcagc aggtacagtg acttgcgtat
    16861 ccaaaacgtt gtattgtctg gttgtcaagc ttcctgatcc gagttgacct gctatcaaaa
    16921 atcccaaacg aatggcataa acctgtgccc aatcagttac tgcattagca gagacgtatc
    16981 gagtaatgac tccattattg ccaataccat actggacttt cattatttcc accccaggaa
    17041 ctaatggctg tattgtccag ttgttgccaa cgagagaggc ccgcattaat gcgctttgcc
    17101 caccttgacc ttgacccaca aaaaatgccg tttgttggat gggaatgaat tgggagccgg
    17161 tttgaaaatt gacagggaag gcactgcttg cattttgtag caccccagcg cctgcatcat
    17221 gagtaatcgt ggttcctgct actccggtaa tttgaaatat tacggtttta ccacaatcag
    17281 agaccgctcc aaacattcca gaggtaaggg acatcccgct agtgccttga actacaaagg
    17341 tattgctgcc gggatcaatc gcggttattc ctgttggata gctgtcagag gtagagccta
    17401 atactaccaa cacatcacta ccttgttgag cattgccaac aagggtggaa tctaatgcgg
    17461 gtgaccaatg actggcattg gtgctatttg caggattgct ttgggttatt gtgaagctcg
    17521 ctccgctgcc attgtaaccc ataaggattg ttggcgttgt attcaatatt cccaaaggcc
    17581 ttgggccccc agcatttagg ctggaaactg cggtgataat ggatccacag ccggcaaatc
    17641 cagccgatct tatcatcggt atgagtaaat tggatatggc attttcagta ctttgaataa
    17701 gatcctgaga gttcgatgct ttgtaaagtt gggtttgtgc caggtatatt tgtaaaattg
    17761 aataagtgag tatcagtcca accaaggtgg ctatcattaa ctcgactaat gaaaatcctt
    17821 gttgtagtct gtacttaata ttcatatctg actctgtact ctgaattgaa cgaaattggc
    17881 agttcctgaa gaagcgctgc ttgcccctaa ctcattttgt gctttactat catcccattg
    17941 tacagtcacg gtaatcacgg tatttcctgc cacacctgaa agagcagacg ttattgaacc
    18001 gcttccattt ggcaattgcg acaccactct ggtgagccaa taccaggtat catatcgtgc
    18061 cagttgattt ggattgcata ccgatgcatt gcataatact gaaggagcac ttacggacgt
    18121 aggataccca ttactgccga tattactgac attataactc ccattagatg cggcatccga
    18181 attcgctcga attctgtcaa aaatatcata gactgcttgt actgcttgca gttttgcata
    18241 actggaattg ttcgctttat tactgactat caacatactg gcgatcccta gcatgccaac
    18301 ggctaatatg actacagata tcaacacttc cagcaaagtc attcccttgt cttttgctaa
    18361 tgtatccatt cggttaatca tattctatct ccgttaaggg cagttcaaag ctccattatt
    18421 ccagactgct tggccgggtg ttgttccgga ttggacaaat ccagtggcca aaaccattgc
    18481 cgagcgggca aacgcattgc ctctgttatc acacatagtg aagttacttt gggttgttga
    18541 taacccatgt gggtcaaaaa caacactgga tacgttcgaa gtgataacag gatcattcac
    18601 tgaagtctgg tgcgatttta aaagcgttgg tgctcctgct actggttggg tcactactat
    18661 ccatcctgag ctccagttgg ctccacaggc cgttgaacct ggtgaaccta atgggcatac
    18721 tgccacattg actgcccgat caagtgccac tccacgcgca tagtttaatg agcttaccag
    18781 tgagtcaatg tttgaagtca aacggctatt caaaatcata gttcttaatg aagggacgat
    18841 tagagttaag atgatggcaa aaacagatag ggttactaat aattcaacca gtgtgaatcc
    18901 ggctatggaa agagggttgc tgttaagagg ggcctcagta tcgaagctgt ctttacaaag
    18961 atcattgtac attatgcctt gtgtttgtat cttttgtgct atattagatt gtgcgggcaa
    19021 cacactactc tcaacgcaaa ataacatatt agctatgttg cgcagagtga atttttgtga
    19081 ttccgttatc actttgttca aagcattatc cctattgttt gatcgaaccc actacattgt
    19141 taattatagt tcaaattagt taaatttttt acaaataatt ggctagcaaa tgcttggtcc
    19201 agttagctat tgcaatttta aggatagtcc ggttagcaag gttgtataga gagaattata
    19261 taggggccag aaacattggt ctggcaaaaa aattttacag tctgaaggct tcatggtgct
    19321 tattaatccc aatccataaa tgattctatg tttaaatccg tttcctctcc ggcaggtttt
    19381 tttgtaagga ttctggtttt atgacaatga tagttttctt tgaatgtttt taaggcatct
    19441 tccagactca caaacgtgta accattttgt ttgtataaat taataatatc aggcaggaca
    19501 taggcattaa gtaagttagc atggattaac aggatttgtg cttgatctgg attatggtga
    19561 tattgcgtat gctcttcagc ttttaacgtt tgttgccaga taaaatccag ataaaatggc
    19621 ttcaactcat ccaggtaagc tctccttcct aattcaggca cagataataa gcgttgatta
    19681 aagacaaaat ctttgctgtc aatggtaatc ggggcaactt gatagtggtg tttttcaagg
    19741 tagcagagga ttttattttt tttacttcca gaactcattg ccaaataggg ataacgaaaa
    19801 tacttgggtt cagtgaggac gggcattaaa agtgaatctg cttctttaat ttcctgtata
    19861 tattctttcg ctttgagttt gtttaaattg gcatgagaaa aagtatggtt gcccaagccg
    19921 aaacctgctt cacgaaattt atgaagtatt tcccaattat caggcctgac ttctttggca
    19981 atgataaaac ctgttgccgg gattttttcg tctttcattg tattcatcat catattcaga
    20041 tgaaagtttc ggtattcacc aacaaagggc aaatcatcaa tagtgattgc tatggctttt
    20101 ttttgagcaa atacgttgtt aaatcctatc aggaaaaata ttgagaaaag cagtaagtat
    20161 ctcgtaacag tcttgatttt cattcgaaat ttaatatgtg taaggtcaat tgagaaaaat
    20221 attgtcacta ttgtaacaaa aattacgatg aatagcttat atttaagagc ttattaaaat
    20281 aagaaatacg aaataaacag ccttattttg aaaataagac tgtttatgaa tgaaaatcga
    20341 cgttaattgt tcaaattaat tatacggtcg gtttgctggt ctcttctgct gtgttttgat
    20401 cttttaagtc atcttcttcg tcttcttcag acatgcttgg tgctttttta aaccagctgc
    20461 ctacatatcc agtgactgaa ctcgcaatgg aataaccctc ttttttgggt tttaatgctt
    20521 cgagttcttc cttacgcttg gtgacacaag cttcaatggc atcagtacga catgttttga
    20581 tcatgttttg tattaacgtc aagacagaga tcaattggaa gccggcgatg tcggaaaaca
    20641 tgttgttttc tcgaacggat tccttagtga tttttttatt tgattgttca ggacgctcaa
    20701 taaccatatg acgaataaat ttattcgcag cagcaatcag tacttccttg tcttcacagc
    20761 aatcagcatt ggcatgaagt atcgtgctta aacctgtatg aatcacagta gcgctgattt
    20821 tatcgttgct taatggatct tttccatact cctgggctat ttggccacga acgatataca
    20881 ttgctccagc aagaatggcc attttctcag acggctcaag agattggtta gggatcttgt
    20941 ctagttgttc tgccatcgtt tgtaaaaatt tcagttgtgc ggagcgggct tgacttaaag
    21001 ctgaaatttt agcaacgttc ttatcagaaa gttcatcgag aattaattga tgaagagcgc
    21061 ctttcatttc atcccaggat ttgaattgct caacaatgga tgaatcgatt ggtttttcca
    21121 catggaagct gttcgcattg gctttggttt ttccatcagc agttaatttt gccaaagcat
    21181 tattttcagc tgcctcttcc tgctcataac ttaatttggc taaagagtat atctttttta
    21241 acgaaaggac ttgcaggaaa tgctcctttt ttaagccttt acgagaatca ttgctttcat
    21301 aaatgaaatt taagaatttg tttaatcctc tgtaaaacat gatgttctgc tcagatgttg
    21361 gtatattttc atcagaaata cccataccat ccattaagcg agtaaacagc aagctgtttt
    21421 ctttcttttc catcacccgc agattatttt caacgtcttt acagacaagc agcatagcac
    21481 cataaaatac atggacatag ctggccaaag aacgtggttt cagttcatcg atttttgctt
    21541 ccaattgatt gagaacagcg gtcagaaatt gaagctcttc ttttctgggg ttgggaaggt
    21601 cttcaatttt gtcctttttt tcgcgttgaa ggatgatttt aacggtgtcg ttaaaatgac
    21661 tttttagtgc atcaaattcc tttaaaacaa acgtcatgtt agtctccaaa aatccaagta
    21721 agattaatgt aagaaatact tacagtggcc agtgtaaact aaacttaagc tgacgtcatg
    21781 tattttcatt atacaattta aaaatatata ataatcattt aaaaattaat aaacttggct
    21841 tgggtgtgaa gatctcaaaa ggaaaaggga ggttagggaa taattggttt tatggaattt
    21901 attcctgggg acgctatgtc cgagatacca caggctcact ccaatgcttc actaaatgat
    21961 actccgattt agttgtttaa ataaaagagg tatattttga gttttcaaac agataaagag
    22021 attcaacaaa aattctttaa ttacaccttg tttgtgttgg ttgttctgct gatatgtcgc
    22081 ttgattgcca attatttttt tcctttgttt gatacgaccg aatcccgtta cgctgaaatt
    22141 gcacgcaaaa tgctggaaac agggaactgg gtgactttgc agcaggatta tggtgttcca
    22201 ttttgggcta aaccaccttt atcaatctgg ttgtctgctt tttttatgca actgttgggt
    22261 gttaatgaat tcgcagttcg cctgccaggt cttttgctgt ctattggtgt tttatggttg
    22321 gtttggggag tggcaaaaaa acagagtggc tcaatggctg cgctcttttc cctattaaca
    22381 ctggcagggt cgctttattt tttccttgat gcagggactg tgatgactga ccctgcccta
    22441 cttttttgta tcacacttgc aagtgtctcg ttttggcatg ccatggttta tgaaaacaaa
    22501 atatggtctt atgcattttt cgcaggattg ggcctgggtt tattagcaaa aggcccaatt
    22561 gctgtggtac tggtcggaat ggctgtattt ttctgggttc tgttacgcaa tgaatggctt
    22621 aatttatgga agagattgcc gtgggttaaa ggcacgcttc ttcttttgtt aattgctgtg
    22681 ccctggtatg ttctggctga attacgaact ccgggatttt taaattattt tatcctggga
    22741 gagcattttc atcgattttt aacccctggc tggtctggtg ataaatacgg gatagtgcac
    22801 catgtaccca aaggaacgat atggttatat gctacagcag gcgtctttcc gtggaatatt
    22861 atagcaggtg gctggctggt gaaacatgga aaaatgttgc cctctctgtg ccaaagtgga
    22921 gacggatggt taagttattt aactctttgg atgattacta cgcttctttt tttcactttt
    22981 gccagcaata ttatataccc ctatgtgttt ccttctctgc ccgcatttgc tttgtttttt
    23041 gctgaaattt tgaatcgtct gaatttggaa ttgaaacatt tgaagtggat ctttatctcc
    23101 tcattactct gtggagtttt atttttggca gtggcttttg tatttagtgt aaaaccaaat
    23161 gttgtgccta atacgcataa gccgattatt actgcatggc taaatcagaa cccaacagca
    23221 ggcagttacc ttgtttattg gggatataag acggatttct ctgctgaatt ttactcggaa
    23281 ggtcaagtta aagcggttaa aaattcgaac gatttatgca aattaatatc taatcggttg
    23341 gaaaactatt tggttatcaa cagtaaagaa actcagcaga ttcgcgaggg attacttgca
    23401 agattcacac tcatcgacac tttcagagtc catcgggata ctttaatatt gttctatgcc
    23461 ccagtgctaa cttgttgagc agtggtttcc aatttcatct cgtagtaaag taacaggact
    23521 tacgaaaaac cccaatctct ttgttaaaaa agtttttaat ttagtcattt agtttatcta
    23581 aactccctta ttaaaaactt ttttgccttg accttggggg ttttcgtaag gcctgagtaa
    23641 tacaaacgag gatggaaata atcaatctat ggcaaaatta tatttctact atgcggctat
    23701 gaatgcaggt aaaagtacgg tgttactgca gtctagttat aattacagag aacgcggtat
    23761 gcaaactctg ttgtttaccc cagccatcga tacacgtttc caatatggaa ctatctgttc
    23821 acgtatcggt ttatccgaac aggcttatgc ttttaacaat tcggataacc tgtatgtttt
    23881 aactcaagaa tttcaactac aaactcagaa atactcttgt gtcctgattg acgaggctca
    23941 atttttaact cgagagcaag tgtatcaatt aacggaaata accgatcaaa tgtctatccc
    24001 agtactagct tatgggttgc gcactgactt tagaggcgag ttatttccgg gaagtcaatt
    24061 tttactggca tgggcagatg agttaatcga attaaagaca atttgtcatt gtggccgcaa
    24121 agcaattatg aatatgagaa ttgatgaaaa tgggcaagct gttattgagg gtgagcaggt
    24181 attgataggc ggcaatgaat cctatgttgc tacctgtcgg ttgcattata aacgaggtga
    24241 agcaggaaag acctttccaa gaaataaatt gttcaataaa gataccaata ctttttaaat
    24301 tttgatataa tttgccacag tttttaggcc ttaaataaag gagaggtggt gtcgcattct
    24361 gtcgcagtgg agtcgagaac ttttgttccc cgtggtgact ttatggcttg ggtagtgtgt
    24421 ctttccgcgg ggcttttctt tttatacgag ttttttcaac tcaatatttt tgatgttatt
    24481 aatcagtcgc ttcgagaaga ttttcatata gatgccaccc aattaagttg gatgtcgagc
    24541 acttatttat gggctgatat tctttttctt ttaccagctg gccttattct cgacaggttt
    24601 tcaaccagga aagttattct caccgcaatg ttggtttgtg ttgttggaac aatagggttt
    24661 gcagttacgg aatcattttt tctcgcctca tttttccatt ttctctcggg aataggcaat
    24721 gcgttttgct ttttatcttg tgttgttttg gtctctcatt ggtttcctcc cagaagacag
    24781 gcattagtca ttggctcatt agttactatg gcatttattg gtggcatgat ggcgcatact
    24841 ccttttgctt atttgaatga tctgtttggc tggcgacgtg cattgttaat tgatggggtt
    24901 gttggagctt ttttaatatt atggatttac atgattgtgc aagacaggcc tgaagagtca
    24961 ccagcacata aattaaccaa tgaaggacaa atactatcga gtttcatgaa agctctatcc
    25021 aataaacaaa attggctggc aggcttatat acctctctac ttaatttacc cattatggtt
    25081 ttgtgcgctc tatggggtgc aagttacctg caggtggctc atcatttgcc tgatattgcg
    25141 gcaagcaatg tcgtcagcct tattttcatg gggagtgttg tcggttgccc attggttggt
    25201 tggttatccg atactcaggg acgtcgcaaa cccttaatga ttttcggagc gatagctact
    25261 ttgattacta ctattcctct atttatcaat gtggttctga ctcaaatgag tctcagtatc
    25321 ctcttttttg ctttgggatt atttaccagc acgcaagtga tttcttaccc cctggttgct
    25381 gaaagcaatc agctagaaaa tactggtgct gcaactggaa ttgcttctgt cattattatg
    25441 ggtgggggtg gtgttgctca agttctgttt ggttggctta tgacacatca cgcgggaaca
    25501 aatgttacgg catatacggt tagcgatttt caatttgcca tgtggatgtt tcctgttgcc
    25561 gcaattgcag gactggttgc tgtgctcatg actcgtgaaa catactgcaa gcgatagaga
    25621 ggagaaataa tgcaggtagt ggatgaattt aaagcattcg atgttaaagc atcgttaatg
    25681 ccatggctgg tttgttttgc agcaaccatg tttttctttt atgaatttat tcagggaaat
    25741 atgtttgctt ccattgcagc aaatatcatg caggattttc agattcaagc agataaaatg
    25801 gcttacttat ccagcatcta ttacttggca aatgtgattt ttctgtttat tgccggaatg
    25861 gttttggaca ggttttcaat caaaaacacc attttaatag ctatgtttct gtgtgttatt
    25921 agtacgttta tcctctccta ctcagattcg ttttatgtgg ctttattttg caggtttgtg
    25981 accggaatag gtagcgcatt ttgctttttg ggtcccgtgc gtttggcttc tcattggttt
    26041 cctccccagc gtatggcttt agtgactggc gcgattgtaa cggttgcaat gacaggagga
    26101 atgctggcgc aatatccctt aactaaactg gttctatatg ttggatggcg ccaagcagtt
    26161 caagacgtag ggatattggg cttggccatg ttagttctca tgtttttttg gattaaagaa
    26221 agacctcaag tcgcaatcaa aaaagaagga aaacctatca atgtgcttgt ggctgcaaga
    26281 aaagcctact taaatgctca aaatttgcga gcagccttat acactagtct tatgaacatg
    26341 gccattgctg tatttggcgc catgatgggg actttatatc ttgaacagag attaggcatt
    26401 agccctgatc aggcagctat gatcaacggt atgttgtttt ttggagccat tataggttca
    26461 cctttgatgg ggtggatatc tgataaggta gggttgcgtg tgctgcctat gaaaggagga
    26521 gtccttgcct ctttacttat tttgctggga gtactttatt tacctgtttc agtgacaatg
    26581 atggccgtat tgttcttttt attaggatta tttacttcag ctcaagtcat tagctatgcc
    26641 ctggttgctg aaagcagccc tcccatgatg actgcaacgg ctgtcagtgt cgtgtccata
    26701 ttaactcaag gcgggtatct tatttatcaa aacctgttta gtgctttatt gacgaattat
    26761 ggcgatgttc gtttgattaa tggtgtgcca gtttatttac tcgaagcgta tcagcatgca
    26821 gcaatcattt tacccatagg attattgatc gcattacttt tattgttcgg attaaaagaa
    26881 actcgttgcc aacaagttga gcatacagta tgaccggacg agtttgcgta ttagtaatgg
    26941 attcctttgg catcggagcc agtcttgatg ccgctcggta tggagatgcg ggtgctaaca
    27001 ccttggttca catttatgaa gcgtgtaaac gaggtgagtg cgatatagac ggggttcgca
    27061 aaggtccttt aatgcttcca aatcttgcgg gcaaggggct ttaccatgct gctatggcca
    27121 gttccgggtt accctttatc gatttatctg ctttagctat tccttctgga tattatggat
    27181 atgccgtgga acaaagcttg ggcaaagata cccccagtgg tcattgggaa atggctgggg
    27241 tgcctgtgac tttcgagtgg ggatatttcc ctgataaatc ttactgtttc cctgaagaat
    27301 taatttcaga atttattaag cagtgtaatt tgccaggagt tttgggagaa aagcatgctt
    27361 cgggaaccat cattatagac gagttgggtg aggagcatat tcgtaccggt aagcctatcg
    27421 tatatacttc tgccgatagc gtatttcaaa ttgccgcaca tgaagaggca tttggtttac
    27481 aacgtctcta tgatatctgc aaaattgctc gaaatttggt tgataaatat caaattggtc
    27541 gagtgattgc tcgtcctttt acaggtaagc cgggctcatt taagagaaca ggtaatagaa
    27601 aagattacgc tacacctcct ccggaaaaaa cattgcttga ttttctaaag gaagatggtc
    27661 gggaagtgat tgctataggt aagattgcgg atatctatgc tcatcaaggg gttacgcaag
    27721 aaatcaaagc cgatggcaat atggctttgt ttgatgccac tttatccgca atgaaaacag
    27781 ccccacaagg tagcttggta tttaccaatt ttgttgattt tgattcttct tatgggcata
    27841 ggcgtgatgt agctggttat gcgcatgccc tagagcaatt cgatgtgcgt ctgccagagt
    27901 tggaagctct attacaaccc gatgacatgg tctttatcgc agcagatcac ggatgtgatc
    27961 ctacattccc tggttctgat catacccgcg agcatattcc agtattgatg tttggccctc
    28021 aggtaaatag caaatttatt ggacggagag attgtttcgc agatattggt cagagtattg
    28081 cggagcattt gcaattatcc tcgccactca ctcatggtgt ttctttttta taattttata
    28141 tccactccct tatggtcgtg gctcggtttg aacgataatc cgagtcacga caatccgagc
    28201 cacgaccgta agggagtgga catgacgatt aatattttga gatattaaga acacttaatt
    28261 tttctctcaa tccctattga ttttttacca atgatcccca tttagccatt tctgcaatgt
    28321 aattgaggat tttattttgg aacaatttca tgggacaaca atactttcag tacgacgtgg
    28381 taatcaggtt gttattggcg gtgatgggca agttactcta ggtaataccg tgatgaaagg
    28441 caatgcacgt aaagttcgga gattgtataa agataaagtg attgctggtt ttgcaggtgg
    28501 aacagcagat gcttttactc ttttcgaacg gtttgaagca aaactggaaa tgcatcaggg
    28561 gcatttgatt agagcagctg ttgaattggc taaagattgg cgaaccgata gaattcttcg
    28621 tcgattagag gcagtgcttg cggtagcgga tagcaaggct tctttaatta ttaccggtaa
    28681 tggcgatgtc attgagcccg aagaaagttt aattgcaatc ggctctggtg gcccctttgc
    28741 tcaagctgca gccagagctc ttatggaaaa cacacagttg tctgccaagg agattgtcca
    28801 gaaagcttta acaatcgctg gcgacatttg tatttatact aacaataatt taaccataga
    28861 agaattaaat gatgaaggca aataatatgg tcatgacccc tcgagagata gttcaagaat
    28921 tagataagca cattattggt caagatgatg caaaacgtgc cgtagccatt gcattacgaa
    28981 atcgctggcg tcgaatgaaa atcaaagatc cagtattgcg caacgagata atgcctaaaa
    29041 atattttaat gataggccct accggtgttg gtaagacgga gattgcaaga cgtttggcta
    29101 atttggctaa agctccattt attaaagtag aagcaaccaa attcacggaa gtgggatatg
    29161 tagggcgtga tgttgattcg atcattcgtg acttaacaga tatggcaatc aagcaagagc
    29221 gtgaatttgc catgaaaaaa gtggagcacc tggcagaaga tgcggctgaa gaacgaatcc
    29281 tggatgtttt actccctcct gctcgaggaa cattaactcc tggtgagaaa aacacgacag
    29341 ctcgtcaagt gtttcgtaag caattgcgcg aaggtgagct taatgataat gaaattgaaa
    29401 ttgaagtagc ggcaacgcct gttggcattg aaattatggc tcctccaggt atggaagaga
    29461 tgactagcca attgcagtct atgtttcaac aagttggaag ctacagaacg aaaactcgaa
    29521 aaatgaccgt tgccaaagca atgaaaattt tgagagagga agaagcggca aaattaatta
    29581 acgaagaaga tatcaagtta aaagcgattg aaagtgttga gcaaaatgga atagtgttca
    29641 ttgatgagtt ggataaaatt gccaaacgct ctgatactgt aagtggggga gatgtatcac
    29701 gtgagggagt tcagcgagat ttattgccat tagtcgaagg aactaccgtt tcaacaaaat
    29761 acggcatggt aaagtctgat catattttat tcattgcttc aggtgctttt catgtagcta
    29821 aaccttctga cttgattgct gaattgcaag ggcgtctgcc gatacgtgtc gagttgtcag
    29881 ccctctctgt ggaagatttt gtacgaatcc taactgagcc aagcgcctct ttaactttac
    29941 agtattccgc tttgatggaa accgaaggat tgactcttac ctttgatgaa acaggaattc
    30001 gacgtattgc tgaagtagcc tggcaagtga atgagcgtac ggaaaatatt ggagcaagaa
    30061 gattatatac cgttatggaa cgcttactgg aagtggtttc atttgaagcg actgataaag
    30121 ctggcgaaac agtgcatgtt gataaagctt atgtcgataa gaatttaggc caactaatcg
    30181 ctgatgagga tttggctcgc tatattttgt aatccagaat agttaaactg aaatcaagaa
    30241 tgaaatcttt ttaacagatc aactttttgt gggggatatt tctcgaaaaa gagatatccc
    30301 tcatgtgatt ctgagtgtac aaccatgcaa tttactttta caaagagcaa ggcaatgcta
    30361 attatattct gatatcatta tgtacataat ggttgtttat attagaaata aatgaaaact
    30421 cagcgaatta tccatcccaa tattgatatt agaaaatttc ctgaagtaaa tgcggatttt
    30481 tccatgaccg atattagcat gggagatttg catgctaatg ccttgctttt cctcaatatt
    30541 cttgttcgcc agggaataat tgctatttct cccgaaaatt atgccaaatt tgctgaaatt
    30601 tatactttgc cagagcttca ggcagattac tgggggaccg aagcacctgt ttttagcgct
    30661 ggaaataaac aggagcgttt ggaggaaatc aagaaacaat acaatgcatt aatagcgcaa
    30721 atcaaaatta ttaatactaa aaaattgatt cgcttgatag gtgatgaatt agtagatcgc
    30781 ggtgttattg actatttcat tttaaaatta ttacaagctt tgcacgatca aggtgctggt
    30841 tttgaaatat tactttcaaa tcatgggatt gagtttgtcg aagcctgtga actattcaag
    30901 gaaaatggaa acaaattagt agcaaaaaga ttaggaaata ttcagcatgg taattcattt
    30961 catgctttgc aagaagcaat agctgctggt gcgatttcaa atgaagaagt attaaatatt
    31021 taccatcaag tttacaaaaa gcacttgaaa attatttctt actcattgga tccagaagca
    31081 aatgaaatta aagtgtttag tcatgctggt attggtttaa atcatattcg agggcttgcc
    31141 cgaaaattta aagtacccta ttcagaagaa tcggctgttg atttggctaa aacaattgat
    31201 gcaattaaca aaaaattcgc agaaaaagca tctgcgggag aaattcacac gctttatacc
    31261 catgatatga tgtatagagg ttatgcaggt gaatatttaa attcaacaga cgaagtggtt
    31321 gccgcaacgg tctggggacg tgaatatggg gatttgattc gaacgtctaa aaaatttaaa
    31381 gtcaccttta ttcatggcca cgatagttac gatcctgaaa aggtagaaca tgtcacttta
    31441 aataatcaac tagggcaatt ccaaaataat gtaggtgatt tatatctgta tgccaccaat
    31501 ggcatgagag cggttccaac acaatgtttg aatccagata aaaaagttta gagtctaagg
    31561 gaaaaaaatc gaccagataa acccaatgat tatgttgtaa aaatccatca tacaaaacca
    31621 tcatttttta aaacggctca tcccaagatg acattccctg acagctacaa acgaatatgg
    31681 gatagcacac caggacattc caatataacc aaaattaagg cattacttaa agattatacc
    31741 aaggaagaca gtatttgggg ttctttctgg agattgatat tcacattgca ctggggaaga
    31801 catcacgtca aatccgtgca ccagattgcg caaacccaat atacatcagt agaagctatt
    31861 ctttcggatt tgaaagcact gaagccaaga gagggcggtt cactggataa acgcattaag
    31921 tttattgagt cacaaattat aactcagagg ggtgataatc cagatttaca attcaatttg
    31981 aaatagtctt tttgtgttgt ctttctctct taagtcatat ttatttgatt tttaaatgac
    32041 catatcattc aggttttagg gactttattc ttgttatcca ttcgattgca ggggttatct
    32101 tggccctgga ataaacctga aatcattaca tgtggttatt tgagtactgt tcctgttttc
    32161 ttgaataatt cttccaaatt aatatccagg gattttttca attcttcatt atgcctttta
    32221 aaggcggctc tccattgttc cgccagagag gctttggaat attgcagctc cagatgagtt
    32281 ggcaagcgat aaggtaataa ttgagttaaa tcatagagag taacgtgact gagatcacga
    32341 cttaacatgt agtgcccatc agcggtagca tggatcagtt catgataaat cagagcattg
    32401 atcatttcat ccacatccac agcgaaaggt tgtttgctgg catccaccag atcattaaag
    32461 cttaatcctt ttccatgctg ttgcgctaac cataactggt gcagccagag taatgcatgt
    32521 gaaaacccat cgagggactt ccctccacga cgctgatggt gcactgaaaa ggcataactg
    32581 atttcagcac ccagcaaagt aataatccat acccaataaa cccaaataaa aaatataggt
    32641 actgcggcaa atgctccata caacaattca taggtattat agcgtattaa ataataagca
    32701 aatgcatgct tagctgattc aaacaagatt gcggcaacca acccacccca aaaagcatga
    32761 cggattttga caggacaatt gggaactact acgtacaaaa aggtaaaacc gattagtgaa
    32821 agaaaaaaag gtgaatagtg aagaatagtg taaggagctc gatgatctgc caatattggc
    32881 atggagaata aataggagct tgctgcaagg ctcaaaccta ataaaaccgg ggcaagagaa
    32941 ataatagccc aatagagtaa aaaggcagag acaccatgcc gagatgaact cactctccag
    33001 attttattca ttgcttgttc tatagtgaac atgaccaata atgcggtaaa aattagaaag
    33061 actatacccc aaattgagag ttttgatact tgagaggtaa actgctgtaa atagctttgt
    33121 acaattttac ctgtggccgg aacgaaattg tcaaaaataa aattctgtac cggctctgcc
    33181 aaaccttgga ataccggaaa tgaagaaaat attgccaaac ccacggacat caagggtacg
    33241 actgccagta agcttgtaaa ggctaatgcc gaggcaatat aggtacaatc gtcttgtatg
    33301 aaatgttgga taacaaaccg aacaaagcga tcgcaggagt agaatttagt ttttactttt
    33361 tctttccaat tcatggatta aatacaagat ttttattgta taactatatc
//
LOCUS       DAXLDT010000016        33305 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXLDT010000016 DAXLDT010000000
VERSION     DAXLDT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 33305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33305
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.016"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..624)
                     /locus_tag="ACF2SP_000629"
     CDS             complement(<1..624)
                     /locus_tag="ACF2SP_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HFD2358434.1"
                     /translation="MAKLNPISFEEALENSTNKNRSILLGNGFSISLCENFDYKYLYK
                     QAQKLADEGEISISKSIKNLFDDINTCDFEKVLDHLNITIETIKHYPKAELLNRTLNK
                     DKDNLIAAFYNTINSVHPKFQSDISPGTFIACLKILSNFNKIFTTNYDLLLYWIIMNR
                     TNQHLDLFNLHDFDFSSLPHDDGFTRDMNTNDFPKWNNNLSRGPQDVF"
     gene            complement(856..6801)
                     /gene="traI"
                     /locus_tag="ACF2SP_000630"
     CDS             complement(856..6801)
                     /gene="traI"
                     /locus_tag="ACF2SP_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFD2358435.1"
                     /translation="MLSLQPLDSADGAAAYYLDVVNYYENDSKSVRWLGDGAKILGIH
                     GQAVEKEQMIALLKGILPDGTQLGRIDKDGIHHRPGFDMTLTAPKSFSILLESGADPR
                     LGEVFDKAVEWFVEEMEKEFAQARQLVDGKIEYIDTRNLVIAAFRQPSSRANDPNSHT
                     HLVVQNMTHCPDGKWRSLASDMDGQKGVVEQIMKHHIYGGLKFRNKLANLTKELGYQL
                     ASDGDGLWEIQGAPEQVLTHFSKRRESIEALLDEKGWSGAKASSIAAQKTKLDKEIID
                     YEKWKEDIIKECQEMGFDPHQLVASSYKPQKNLFQTLKENIVERFYGKDNIEMTHARE
                     SVYVAIESISQQHAVFEERELKKEALKYVIASNKIIDETLINKAIDENIANQNLYTAR
                     HPFTQKPLLTTPWQLTLESEAIQRIENGKGAVSAICSKQKVSEFIKAKEQEMAFALSP
                     SQKKAMTTFLTSTDRFIAIQGYAGTGKTTMLRLTRELASLQGYEIRGITAGSAAANEL
                     RHKGGLNASTFARELGRLQNQKQDLSKTIFVVDEASMLSNPQGHKIIKLAEQFNTQLK
                     IIGDKAQLPSPSSGKLFSVIQDYGIKTVAMTDNLRQKDSELRESAIHAGRGEIYDAVD
                     KLTHVETLDTYLKRVDYISSKWLSLTPEERQNTLCFAPTHRNRQDITQILRNALIQEG
                     TLTGQEHLQPILKERNLTGIKLRNAAYYSQNDIIRFNHTIQRYNIKAGDYLTVGQVTN
                     KNKHNNTLILKHENGQAIKFKLSSLPEFRTENKDLERPVEVYRREQLSLMAGDQIQWK
                     RNSEHNGIRNSELATIRQINNEGITIITEDNQTLHLPHGAKELRHLDHGYVLTTYATQ
                     GKDKKRGLGLIESQNRFASTIQNYYVETTRGIWEMIVVTDDKDHLVKAITTNNSDKYS
                     SMDMVDSDTLKAHEARFKEHKNSIVLQNAIEKKLSKEQDWKGLEQTVETYVQCKQQGE
                     DRKAAKLAFAIVNDPKLYRLAKERLGFGVSTYRREALRFQTSKLLHSLPKSERQDFST
                     VRQYVFLNQQILKRSQHINAQSLDNGISNQNKQALQQQSAKRNAVAFVISRDLERYKP
                     YLQHFSIGELNRIGLPQHEYGKEFKKAQMRLESLGKQATRDLIRTNISLYLNAQGEEK
                     ERLAVQIKRESKLSHSFVLTHAKELNQKPESLWQSIHKDARLHSDKLFRNGLSAEGRL
                     AFDNIKAYKALQLELRENWSASLKEAGNSEGKSSNPKSIELLTRRNELAHELMQNKAM
                     PEIASYFKLDLAKLAVQTEKQQYRENIKQFTASKSNFKTRLAVINEIKNDITGHYPFI
                     KEANIESKTLSKYLRVTDRKERLSNISSPEKKDYQCFLTYKKASMQTYRLWQQAHLNK
                     ADGNVQNNKLISAAISQSSKRDALAHQLKGSKYLDSILSYEKGNKEKLLAQASNHQIK
                     LREIKELNEVVHTLSMQFPSVTNSNSAKEISAWKKNWLSLSSHMKQIEKGSGYQLALQ
                     EYPLNVSSVNTINKELENNYDFKPETITQKSLQSTNPVLQKIQQSAQFLDARIINEAL
                     MADPETTYKTIWGEPKTQNSRELRYSGGLIVSLKGKDKGLWHDFSDGIGGAPIQAIIA
                     RDKLSFKEALSQAASMVGINQLENLSIFIKAPISSKNELQKLNDLEKKNKVISAKSIW
                     DGSILAKGTLAEKYLKQHRGIESIDKMDIRFWPTGSQWKNCNEQGLLEDKVNKIPALI
                     IAARNEKNELTGVQRIYLDKQTASKNKFMDNPKLSKGIIDGSCGVIQKGMQGSRLYIA
                     EGFETGASIALADSKATVLCSFGVSNMKNLSPIIKKFHSKEVVIAGDNDGEFAKSQQA
                     IEKTINAFKQDNLSARAVFPDGLQGKAKTDWNDIHLSKGIAEIQKQLMRKDIGAISSI
                     VDKPIELPKTIADFVQIKSMKLSGSQIISIRDNAATIDNKRDLNQLVAAYNKSQQALE
                     KSGSFTPEKTLPTKTRSEIEMDR"
     gene            complement(6822..8711)
                     /gene="traD"
                     /locus_tag="ACF2SP_000631"
     CDS             complement(6822..8711)
                     /gene="traD"
                     /locus_tag="ACF2SP_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFD2358436.1"
                     /translation="MAVNPTKLFIRGGQIFLHNVRMFTQVGKKVSLAMLIVFFIFSVI
                     AFYFNTSAYQRYIGQQWVKAQAMTLIQSKAKQVVRMPTGESYPVYSAQIVKAPMVIGI
                     VNQLKQALLKSMIEALFASVMMLIVMVKWLQKRGENQSKAKIIRGSELVDENTLQKCI
                     KKTSRISPYRIGGVSLPFGSETQHIQIVGTTGSGKTVAIRDLLTTIQARGERAIIYDK
                     GGTYLSRFYREHQDVILNPLDTRGHSWNVWAECEDKADLEALAEAIMPMPINNTMDPF
                     WIKAARMIFVSTANELKNDPKRSNLMLLQYLLTADLGRIHHLLRHTESESLVSDKVQK
                     TALNVKTVMATYLKSLLYLKDDGDIFSIRDWILNEQNNSCLFVSSDGRKHPTIRPLIS
                     AWINTATKELLSLAPDDNRRVWFILDELATLHALPFLAAVKSESRKFGGCFLLGHHGA
                     SQLRTIYGNDGAASLSNLCSTRLFLRLPEHTDAEHASRNIGTYEIEEVNESISYGANT
                     MRDGISVSRQTKEKQLVLPTQIQVINDLQGYLRVKGEFPAAKIKLNYVDYPLIHEEFI
                     ARDIDPDPLRQKVEQLVDTYSDPILAASHDDALEAATSTKKDITKPIAREKKLEEEKE
                     LVEFL"
     gene            8779..9243
                     /locus_tag="ACF2SP_000632"
     CDS             8779..9243
                     /locus_tag="ACF2SP_000632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358437.1"
                     /translation="MEKNYKGQVMISSLDELSPEQRKKLRLASMGIGSLVLLIVVLLF
                     LCFGDFPSNKDSLFFYCTDWLLFLVSPLAAFGLGASFGFDLMLSDLGFAFSFGSYGGS
                     SLNQPFSSHESPNYHRSNHYGSSVSINPSSGRPMSGSSGMDTSGNPYGTRSW"
     gene            complement(9247..11967)
                     /locus_tag="ACF2SP_000633"
     CDS             complement(9247..11967)
                     /locus_tag="ACF2SP_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFD2358438.1"
                     /translation="MAVTMQIYTITGGELLKAFYNATAAMFHYDGILGIFRTALIIGG
                     VWTVGQFIVKRDVRSMFFFILKYAFVISFILTPTCNLQIHDRTDPLRPDLTVDNVPLV
                     LGVVGGLSSQLSDKITRLFEFFFHSPNDMDYSQTGMIMGAKLFLSASNIKITDPLFNA
                     NMQKFMQQCVFYDVMYGRYGLKDINQARDIWELVKSNASVARGFIYDGSFKSCMEAAS
                     LLDSAWSGVIEDAKSKYAGFAFGNHSDALANFEKYLPQAYNHLTAMSGDAAALIRQNM
                     MANAIRDGVFSMGARVNSKAAIDSFSASRAQDKIPAALSNIGLMSAYWLPMLQSLLFC
                     IVIGCFIFVLFFFPFPSGISFFQFYATLYVWLALWAPMFTVINYIMTILAQFSLSFVS
                     TGATTLAYQTGMNQVYENMAALGGYLVLATTTLSYMLISRHVGSFVSLTQQPTGIVQN
                     AASSAAEEAQMGNYSYGNTSFANHNGFNSSSFHNDQNARISLGGIETSLDSGSIARIS
                     RDGSETLTMATATSHTPLNIQLGESTRSAFSELSDSAKSAGFNKSIASSEHYAASLRS
                     LAELGETQSHGEQSGQGHQISTSAGFNAAASKISNLIDTFAHDHNISRDDATKVLSAA
                     SINLGGSFGFGTGSAVPGAKGNIEIGGNVRHERDKSESHQDARLMNEARRFSEEHHFT
                     DVVNEARQATKDDHFRTSDDFSGRLANNFSAGFDKSMQYRDEAVASFSESESYHRQAT
                     TSSEQTASINLDAQTGFIDWLSHHRAPNSQGTIGLQQAEWLIRHDPELAQSYARQFVG
                     EKTAQSISQFQKNHPINESQVHQKNEGFKSRIAGSAGVDHALGDYTNTFNDRAKNINA
                     GSVNKDSVSAVDKDFARAKSEMSKNVEGIVQDGSQVMQDVIVAQANKDVK"
     gene            complement(11973..13358)
                     /locus_tag="ACF2SP_000634"
     CDS             complement(11973..13358)
                     /locus_tag="ACF2SP_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFD2358439.1"
                     /translation="MKKILLSCGLIGALGIGQVYSKSIGEDLTGYFKALNMGANVTEP
                     HAYQAQRAGFYTGGNLYARSSVRNLQIMRLSWPKVSAGCGGIDATLGGFSHIKGKEFV
                     DFTKNILNNSQGLAFQLALEQATPLLGNVATKLQNVATWVNQTNISSCATAESAVLGL
                     APRTRIAQQHACQSIGQSKNLFSDWAEARQGCGKGSSNYDYDRVINENSSNEQVVDNT
                     NVAWNALKKNGLFADDIELAQLMMTLSGTLILTRDAEGVHVQRLSSLVSNNNLISALL
                     RGGEVKIYQCDEKVKCLQPTLTSKNIDISHGLESKVRDLILDMASKIKDNVEQSEAAK
                     GLIESTQYPVMKMVSVQMAFMKDSAVIDTTRYSEAIAIDILFQYLNENLQLVKQAAGA
                     LQYPEAIMKEFQTDLTQARRDLVQMEGSAHQRMSMAMQMIQETQTIEQMLVGEFSSEL
                     TQSLSWANQMR"
     gene            complement(13355..13777)
                     /gene="trbB"
                     /locus_tag="ACF2SP_000635"
     CDS             complement(13355..13777)
                     /gene="trbB"
                     /locus_tag="ACF2SP_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFD2358440.1"
                     /translation="MRNKLAIFLILGTLFSSSLYADIDNAPLHALAKHQGFFFFFSSS
                     CPHCQRFAPTLKRLSQHYGFSVVAISVDGGFLPSFPDAVMNEGQTKVFQVSVYPSLFL
                     INPKSQVASLVTEGTIDEGELTNRLLKISQIHDKEVPL"
     gene            complement(13767..14549)
                     /gene="traF"
                     /locus_tag="ACF2SP_000636"
     CDS             complement(13767..14549)
                     /gene="traF"
                     /locus_tag="ACF2SP_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFD2358441.1"
                     /translation="MARWFFVLLVSVSPFLYAEQPYLNEHEEGWYWHKDPKEEVKNKP
                     QTESVAPSHVSKPADPDKTWKLIGNRVQQARAQAILNPTPENIARARRLQRLIVAQAN
                     LFSEKWMLDLLINPDQDESLINPSSSAARDIYNQQNSMQKEKAITQISQTSGLLYFYE
                     GGEPFSERMAQVVSDFANSYHMTVIPIAMTNRISPMLPNSRVDSGQATQMGVKHIPAV
                     FALNPVSKKTMPVAYGLVSQSELKENILMASNAFQSGDYNAQ"
     gene            complement(14542..16353)
                     /gene="traN"
                     /locus_tag="ACF2SP_000637"
     CDS             complement(14542..16353)
                     /gene="traN"
                     /locus_tag="ACF2SP_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFD2358442.1"
                     /translation="MKGFVLVFLILCSQVFALDLNQAYQEGVQTGTSHTNQSIDLLKA
                     LDLSQFPGYQANLPQEHYYSGVTQASTGLEADSQTAVAQNDAGKAVGESFNHRPLYQV
                     NPASESMQKLNQIAENGDAIMHGQNTDKTTCSLKPKECHYSWQEKTCLSSKVLGVLHC
                     ARHLRLDVSPYKTESYSLYLRKGGMRNTPFKVSVNLAQADTCQQGKTPCYTIYKDLAP
                     APAILLPANCAMVKVSFLDEKKLVVVEQTATCANPTLSLSVGNCRFGRCTVPYVHSVS
                     MTVEIYESKEYWDDQCQHLQNKEKEGLCHITEPLTCTEPNQTRIIGDVPLTRPCWKER
                     ASYTCGGSQEQNTCDNLVNQGCEQTASTCVKEEAGLCTTHQQTYQCPINQCTDNQLLC
                     GEDAFCLDGNCAKHELTPANEDDFKKAMSTLSAASDASKDFDGNANFIFKGQLLECST
                     TMLHFKNCCSDKGWGLDLELAQCSDAEKKLGKARENKLVVPTGEYCFKRKKLPIGSVC
                     VDHHQTYCVFQSKLARMVQVQGRRDQLHIGFGQNKYSNCSGITPEQLQLIHFEAIDFS
                     EFYEEVKNKQKQPDYQQTANGISQRLNQFYNQGDING"
     gene            complement(16353..17021)
                     /gene="trbC"
                     /locus_tag="ACF2SP_000638"
     CDS             complement(16353..17021)
                     /gene="trbC"
                     /locus_tag="ACF2SP_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFD2358443.1"
                     /translation="MKTIRMNTSFLVALFLLSFPALAEDEAYIASIEANTKNRANAFT
                     NEIKQISKSLETSEKSGQIAEFTGEMHQVIKTQEPIVTHLKKVSQVLVFLSFSMPEKS
                     LKAWLLQCKKSGATPVIRGLIHNSFKETMTAIQTLSKKTGIGMQLDPILFKTFDIAMV
                     PAVVYVKDTPACPANMDCKPVSYDSLYGDVSLDYALEKMRGDEQSEDPVLDQMILRLR
                     GELI"
     gene            complement(17037..18032)
                     /gene="traU"
                     /locus_tag="ACF2SP_000639"
     CDS             complement(17037..18032)
                     /gene="traU"
                     /locus_tag="ACF2SP_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFD2358444.1"
                     /translation="MKAFFILIAFLASGLTQAGSCHGRFVNPITDVCWSCLFPFSLGQ
                     ANLVSSNHLPDTKNPKLPVCECPGNPIPRLGLTMGYWEPVNLVDVTRTPFCLVNLGGI
                     SLNFGKYYRKGAVETDSNLSNQSFYHVHWYKFPLMYWLNVLTDGICVEKGDFDIAYPS
                     ELDVMWNDDELGFVINPEAVLFGSLPAQAACAADSSTALLGSAIDKLFWCAGAQGSMY
                     PLTGHVQEHVGGVQASVLLSERMNFKLHRQGLLEDTVGLDDSTSSAKSICHTHYSPIL
                     PKSRYRYQMVNPLPTTNACYPLGRATTLWEAGHEYPFKGEDFGYLIWRKRNCCAF"
     gene            complement(18029..18637)
                     /gene="traW"
                     /locus_tag="ACF2SP_000640"
     CDS             complement(18029..18637)
                     /gene="traW"
                     /locus_tag="ACF2SP_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFD2358445.1"
                     /translation="MKKLLLIICMLYLPFAEAKDYGVFGKTFEIAEIDFLEYIQQKMK
                     GMQVNGEWKTVQSEFKKRVKEHVARPTPIRLPRAEDNRTWFFNPSLAVPYDVKDSHGQ
                     VIVKQGTVINPLDRVGLSSTLLFFDGDDEAQVAWVTQELTQHQKVKLILTSGSVKEVA
                     NQLKQAIYFDLNGFLVAKFQIKYLPARVFQAGKRLQINEVTV"
     gene            complement(18634..18975)
                     /locus_tag="ACF2SP_000641"
     CDS             complement(18634..18975)
                     /locus_tag="ACF2SP_000641"
                     /inference="COORDINATES: protein motif:HMM:NF021213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI F-type domain-containing protein"
                     /protein_id="HFD2358446.1"
                     /translation="MAMFKTTQVSLLTALITSILVNGIFFSALRRPDIATIDILSITS
                     QFIKEEAQKNHSNLEKEAEIKAFSHRLESALQDLSHSKSLVLLPREAVIKGSTDYTAT
                     LMTMIKLEHKS"
     gene            complement(18962..21514)
                     /gene="traC"
                     /locus_tag="ACF2SP_000642"
     CDS             complement(18962..21514)
                     /gene="traC"
                     /locus_tag="ACF2SP_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFD2358447.1"
                     /translation="MLSTIKKARLAVKKTSTILSHATGSLLETLREELGMTDAHHKKQ
                     IEALLDTYCLAPYLPDEVYDSEADIYPYKQASHMMFECSTLIGASEETVTILTSILTD
                     ILPPESCFHALFWASPKIGHMIDEMEHQRNQGSEVLKALAHRRAEFYRKGVYSSLTKE
                     SNFVLRDFRLFLCISIPRKSIEDDRLLLGSLREDISNNLKSVNIICKELSIEEVISLK
                     REWLFPTDSLYEEKTNYDTNTEIRFQLTDIEASMRVKSNQIEIEREEDTRVIKCLSVR
                     QYPKSPVQWQLGDLFGKMFNTSLQISCPFLFSFAIRIKDKDKVMAALDAKYADEGQNA
                     KQPFIAKWVKNFNKKYAEIEELRERLSGDDSLVDSYYNVLLFTTPANVRRDVRRAKDV
                     FRGNQFDLRTPTGLQLQTLLASMPFAPAEGMFDDYKSFGRVRPLTSFNTVNLLPIQGE
                     WKGAGRFAQVYPQRRGQFTAIYFFDNVVRNYNVAIVAPPGKGKSFLMNDYIQSLLSQG
                     GFVYIIDVGGSYAKSCEMLNGQLIDFNHDTEISLNLFSTIDAQAKNDTAFKDVHNQLI
                     QLVGMMARPSGNVSDEERSHIEKAIEAMWRQHHNATTITLIAHHLESSNDPISQNIGR
                     LLYSYTKNGRYGSYFEPPCSLDFKRNLVILELGGLDGNKDLQRIVLKILMYQITNAMY
                     YTPKHIPKSCLIDESYELLSDNGTGGSANFVNEGYRRAPKYGGNFITVTHGLDDYENN
                     PTAKMCYDNAYYKIFLGASKSTIDTLKTSNQLDAYGERLIRSLKITNEYSEFLMMCEE
                     NSTVHRLIVDPFSRVLNTTRGPEVEALKRLMATGLPLADAISKVALEVYGYV"
     gene            complement(21526..21876)
                     /gene="traV"
                     /locus_tag="ACF2SP_000643"
     CDS             complement(21526..21876)
                     /gene="traV"
                     /locus_tag="ACF2SP_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFD2358448.1"
                     /translation="MKILTTLLLLPCVVLTGCAKLNEDFDCPAPNGGTCKRMDQVYDM
                     VNGKGKSLAVTPSRNPLVMDGRPGQPVRYGEGVMPLWIAPYEDTDGNYHQANRVYSVV
                     KEGQWMQQSQLALK"
     gene            complement(21890..23176)
                     /locus_tag="ACF2SP_000644"
     CDS             complement(21890..23176)
                     /locus_tag="ACF2SP_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/VirB10 family protein"
                     /protein_id="HFD2358449.1"
                     /translation="MSIQLKKQQQIFLGIVIGVIVLVIACFALLGNKTVKSREVDKPA
                     YKKHSLASPISTATNESHWIEKTQNAWRAELAKSELFDKNLKALGDEKDKQSKLITSQ
                     KDELSEMKSTLSELKHQIVKLQMERTTPIDGTLKNNEPQNGLLPRADEAAASNSMEMD
                     SGFIQYTAKLNKKSKPRIKTPNNYVFSNTFAKAVVLQGADASAGVLSQANPDIMIFQI
                     VDDGVMPNKYRAPLKGCMVSASVIGDISSERGKFRTERLSCIHSNGTNIDIQVTGIIS
                     DSKNGIRGRAVWRDEPMVKKAFWGNFWESMGNIGQQYSTDYSTSPLGSVSTIQAGKIP
                     LAAVSSGGSGAAKMYAQYNIKRAEMYHPIIQLPPIVTVDVTFLKGFWLDGGTDTNAPE
                     QPEESQNSKVAFEAPAESETAEEKAANQFFYKNHSF"
     gene            complement(23178..23891)
                     /gene="traK"
                     /locus_tag="ACF2SP_000645"
     CDS             complement(23178..23891)
                     /gene="traK"
                     /locus_tag="ACF2SP_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFD2358450.1"
                     /translation="MKQMAMIFSLGLLIATAEGAQIKTVRNYEDIFINASIKEPNNLM
                     LEDDRIQQIKAPGNTLVDACNGKANCKLIDDTTGILTFLPSPLYHTRAFTINLLTEKG
                     LFYNVRVEPKPIASQTIVFKSYRNPVIRARAPHTSSYEKVMVGFLRALVNGYLPEGFT
                     QTIPKNPSVYKAQKTQLKRLLTVNGNRMRGEIFELKNVTNQPIDAKESWFNWPGTKGV
                     ALAKTHLAPFETTRLYRIS"
     gene            complement(23894..24457)
                     /gene="traE"
                     /locus_tag="ACF2SP_000646"
     CDS             complement(23894..24457)
                     /gene="traE"
                     /locus_tag="ACF2SP_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFD2358451.1"
                     /translation="MKIEQKKSELMKEKKIRVLFIGISSVLLAMTFMQSITIVVLLHY
                     AHSKHETHFIPPKISQEFTLSGTGVSEGYLRDMTNFLTQLRFNITSSSATYQFNALLG
                     YVAPSLYGDIRAQLVKEVEQINHEHLSSVFYPSTFEIDIKHLSVKVMGQMKRFVGADL
                     MSDVRETFLIRYSYEYGLLKLTNLEKV"
     gene            complement(24461..24754)
                     /gene="traL"
                     /locus_tag="ACF2SP_000647"
     CDS             complement(24461..24754)
                     /gene="traL"
                     /locus_tag="ACF2SP_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFD2358452.1"
                     /translation="MEDPRKYKTPQYLNEPYRLIIFTLDEVMVGALTLYVFGAVCGFF
                     VTSLILTCAFLFLMKRMKGNEGPYFYIHLFYWFFSLSPKLRATPPSWIRAFLG"
     gene            complement(24756..25058)
                     /locus_tag="ACF2SP_000648"
     CDS             complement(24756..25058)
                     /locus_tag="ACF2SP_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFD2358453.1"
                     /translation="MSLLNQFRSNVLSSINAHIGKIFILICLLIMAQGTFAADALSAA
                     EEVVKDTYNGSIKTYLYVGEAVAAVVTLMFTRNIKTLGGVGAMAIFLNVVAMLAGV"
     gene            complement(25073..25309)
                     /gene="csrA"
                     /locus_tag="ACF2SP_000649"
     CDS             complement(25073..25309)
                     /gene="csrA"
                     /locus_tag="ACF2SP_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFD2358454.1"
                     /translation="MLSLTRRVGESIVIGEDIFITVLCCKGNQVRIGFNAPNSVAIHR
                     YEIYQKIQSEKHDGLADPTKKFCPSMQQSILNHH"
     gene            complement(25323..25700)
                     /locus_tag="ACF2SP_000650"
     CDS             complement(25323..25700)
                     /locus_tag="ACF2SP_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HFD2358455.1"
                     /translation="MTTSSTLAPTEEGRKRIDRLFLRFAAMYGQVWRSQFKSDEFLVF
                     VKGEWQQGLFTYADNILDMAIDLCRKNKELPPTLPQFIDFCKNCSKRSSFFVPDAAPK
                     NNNPEVAKTQLLKMKHILNMKVN"
     gene            complement(25648..26532)
                     /locus_tag="ACF2SP_000651"
     CDS             complement(25648..26532)
                     /locus_tag="ACF2SP_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358456.1"
                     /translation="MDFLINDKELAALCGMPHLQQLAYLRGIRPYMDVKTGLVGIKRG
                     ISYQSIAEQLYIEPHQGIKSGSYSRMQIRRALSALGRAGLITPQSEGLKLILRCELAT
                     MGYSVQNKLDLNQTQQLDPAKNQQRLINTGLSKTDPLKAGIDKSPKADTPLKEDNYYI
                     FLLSQFEKFWDLYPLKKSKQKAWEAFQALNLTPELPIQIYSALQQQIQFYQQQHSQGQ
                     WVAAWKYPANWLAQHCWEDEIQSDTCQETNNAVHETNYKKQPIRDSFWESCKSGIEEE
                     TDDNIIDLSAYRGRSQAH"
     gene            26705..27385
                     /locus_tag="ACF2SP_000652"
     CDS             26705..27385
                     /locus_tag="ACF2SP_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2358457.1"
                     /translation="MNIKEKIGQRIQEERIAKGLTRKALEELTDDLKQSRISNWERGD
                     RTPGPEEIKQLARALDISPAYLMCLTDEKRPKKIPGLGSLVPLLDHQQACDPKGFIQR
                     VRNEHNSDEISFIPISSELSTRLGENAFALKMKDESMHPELRVNDILIIDPNSELHPG
                     SLVAAQLNEENEVIVRRYKQLTLSKEFNPFELRAENDNWGNIKIDKVTDAIIIGVIVC
                     VVRYTGRY"
     gene            27534..28208
                     /locus_tag="ACF2SP_000653"
     CDS             27534..28208
                     /locus_tag="ACF2SP_000653"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2358458.1"
                     /translation="MDMLDLYCDYLIFQNKYATATGLSDLVDGAFAHNKVTRFLRFEH
                     FGSKSLWHYVKKPVRSQETVGGVLILDDSIEEKPYSDENEVNCWYYSHAKGAVLKGIN
                     ILYCMVRYADFSVSIGYKVIKKDIAYCDIKTRQTRRKSSVNKNQLFQSLIAQAITNKV
                     MFDYVLADNWFGSKANMVHIHKDLQKSFIIGIKSNLTLALSENDAKNGRYQQVRALEL
                     EEDILA"
     gene            28657..29229
                     /gene="msrA"
                     /locus_tag="ACF2SP_000654"
     CDS             28657..29229
                     /gene="msrA"
                     /locus_tag="ACF2SP_000654"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFD2358459.1"
                     /translation="MRYLFILIALLSQPLFAKPSEAIFAGGCFWCVEADFDKVPGVLE
                     TISGYDGGTEPRPGYALVSSGKTAYVEVVRVVFDSDKVTYQELLDFYWRHIDPTVQNQ
                     QFCDVGKQYRSVIFYLNDDQKKAALASIDEIKKRFPKVYTEVIPSTTFYPAEEYHQDY
                     YQKNPIRYKYYRYNCGRDARIKELWDEKSS"
     gene            29213..29710
                     /gene="msrB"
                     /locus_tag="ACF2SP_000655"
     CDS             29213..29710
                     /gene="msrB"
                     /locus_tag="ACF2SP_000655"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFD2358460.1"
                     /translation="MKSLVSFIVSVFMLNTALAYTPEFNKSEKLKQLTPLQYQVTQKA
                     ATEKPFDNPYWNNEQAGIYVDIVSGEPLFSSLDKYDSGTGWPSFTKPINTANVILKPA
                     RHYLFFVQTEVLSKNAQSHLGHVFNDGPKPTGERYCMNSAALRFIPKDQMSKEGYEEY
                     LTLFK"
     gene            complement(29703..30290)
                     /locus_tag="ACF2SP_000656"
     CDS             complement(29703..30290)
                     /locus_tag="ACF2SP_000656"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFD2358461.1"
                     /translation="MIEQHLRPLYQRLCVNPITPVLIERVTPNQVTFFSGVLGLLVIP
                     ALWLNLPYLAISLLLLSGYCDTLDGTIARLTNHSSDWGSVLDIMTDRVVEVGVVFALW
                     AINPNERGLGSLLMMASILLCITSFLVVGIFKTNDSEKSFHYSPGLIERAEAFVFFIA
                     MMLWPNHFLSLAMIFSLLVIGTAMHRLYEFHKQSI"
     gene            complement(30295..32430)
                     /locus_tag="ACF2SP_000657"
     CDS             complement(30295..32430)
                     /locus_tag="ACF2SP_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2358462.1"
                     /translation="MKISAIKTWLPLLIIIVLLGLFLGLGLNKYVSFASLRDNHEWLI
                     AITKSHFYLVSFVFIIIYTVAVALSIPGAIFLTLIGGFLFGILWGTFLVVLSATLGAT
                     ILFFAVQSSLGEVFSKRASGWVKRMRSGFKDNAFSYLLTLRLIPLFPFWVINIVSAVL
                     GVSASTFIIATFIGIIPGSIVYVSVGNGLGELFAASLQPNLGIIFEPKFILPLLGLAA
                     LSLIPVFYKKKGKQGTEKMKDKDLFCDLAIIGGGSAGLSIAAGCSQLGLQVVLVEPNK
                     MGGDCLNYGCIPSKTLLNTAKIFYQTKHSTVLGIQAKSVKIDFLQVMQQVHNVIACIA
                     KNDSVERFTSLGVQVIQEAGHFIGPKQFKLKRKIIRAKHFVIATGSSPAIPPIQNLNK
                     VSYLTNETIFNLKVQPEHLIVIGGGPIGCELAQAFAMLGSKVTILEAFTILPKDDADC
                     VAIIRSQLESMQIALYEQIKINKIEESADKTISVHLENQDNPITITGSHLLVSTGRVA
                     NVDGLGLEKAGVQYTTRGINTNARLQTTNKKIYAIGDVTGPYQFTHMASYQAGIALRN
                     IAFKWPAKVDYKAVPWVTYTEPELAHVGLLASEALKDPTLKITEFAFAEVDRAQAENK
                     VNGKIKIITGKKAKILGVSIVGAHAGELILPWVIAVKERKSLRTFTDAIAAYPTFSEI
                     SKRVAGEYYKPFIFSSKIRTFVRWLNKLG"
     gene            complement(32449..>33305)
                     /locus_tag="ACF2SP_000658"
     CDS             complement(32449..>33305)
                     /locus_tag="ACF2SP_000658"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HFD2358463.1"
                     /translation="QYYLVLILIFIYLSLFVLEKKWPLRKATRPLLPRLAINFTFTLL
                     VYVIASLFISPLAKATVLFTFSHDFGLLSMLPFNQWVVFILGFLLMDLSFYYWHALNH
                     KLPLLWRFHVVHHADPDLDVSTAMRFHCVEIMYSSLFRLVQLGLIGVSPLLFFCYEFF
                     FQAHTFFQHSNIKLPFRFERLINKIIVTPRMHGIHHSNYLNETNSNYGVVFSFWDRLH
                     QTIKLDIPQQAIIIGVPGYSKPSDNSLIRLLLMPIKAQRHYWQIKKDFHFSRKSKTSD
                     KKVRTLCE"
BASE COUNT         9577 a         6827 c         6426 g        10475 t
ORIGIN      
        1 aaaaacatct tgcggacccc ttgaaagatt attattccat tttggaaaat cattggtatt
       61 catatcacgc gtaaatccat cgtcatgtgg taatgatgaa aagtcaaaat catgtaaatt
      121 gaacaggtct aaatgttgat tagtcctatt cataattatc caatacagta aaagatcata
      181 gtttgtagta aatattttgt taaagtttga taatattttt aaacatgcta tgaaagtacc
      241 aggagaaatg tcgctttgga atttgggatg aacagagttt attgtattgt agaaagcggc
      301 aattagatta tccttatcct tattgagagt tctgttgagt aattccgctt taggataatg
      361 ttttatggtt tctatagtta tatttaaatg gtccaacact ttctcaaaat cgcaggtatt
      421 gatgtcatca aatagatttt tgatgctctt acttatggat atctcccctt catctgctaa
      481 cttttgtgct tgtttgtata gatatttata atcaaaattt tcacataaac taatactgaa
      541 gccattaccc aacaatatgg atctattttt gttggttgaa ttctctaatg cttcttcaaa
      601 cgaaattgga tttagtttgg ccatcctttg ccctctataa ataagacccg atgtgtttaa
      661 ttatagttca aacgggtatt ttttgagtct gaggcactga attaccgaag tggttatgtc
      721 ccttgattgt cccaatatcc tttgggggtt ctctaagatt atgtgtttaa aacagaaaaa
      781 taatatgaac acctgattta gtatcaatgg cagatatttt cagtgtgagc atacgctatg
      841 ctatggttgg tgtatctaac gatccatttc tatttcactc cttgtttttg tgggcagtgt
      901 tttttcagga gtgaaggagc cagatttttc taatgcttgt tgacttttgt tgtaagctgc
      961 gactaattga tttaagtctc ttttattgtc aatggttgca gcattgtctc taatggaaat
     1021 gatttggctg ccgcttaact tcatggactt tatttgtaca aaatcggcaa ttgtttttgg
     1081 tagttcaatt ggcttatcaa cgattgagga aatggctcct atatcctttc tcattagttg
     1141 cttttggatt tctgcaatcc ctttgcttaa atgaatatcg ttccagtctg tttttgcttt
     1201 tccctgcaac ccatcgggaa atactgctct ggcacttaag ttatcttgtt taaaggcatt
     1261 aattgttttt tcgatggctt gttgtgattt agcgaactct ccatcattat ctcctgcaat
     1321 aacaacttct tttgaatgga attttttgat gattgggctt aagttcttca tgtttgaaac
     1381 gccaaaggag cataaaactg tggctttgct gtccgcgaga gcgatggaag cacctgtttc
     1441 aaaaccttcg gcgatataaa ggcgggatcc ttgcattcct ttttgaataa cgccacaact
     1501 gccgtctatg attcctttgg atagctttgg gttgtccatg aatttatttt tactggctgt
     1561 ttgcttgtcc agataaattc gttgaacgcc tgttagttca tttttttcat ttcttgcagc
     1621 aatgattaat gctgggattt tattaacttt atcttccaat aatccttgtt cattgcagtt
     1681 tttccattga gatccagtgg gccagaaacg tatatccatt ttatcaatgg attctatgcc
     1741 ccgatgttgt tttaaatatt tttcggccaa tgttcccttt gcaagaatag aaccatccca
     1801 gatacttttt gcagaaatta ctttgttctt cttttcaagg tcatttaatt tttgtaattc
     1861 atttttactg gaaatggggg cttttatgaa tatggaaagg ttttctaatt gatttatgcc
     1921 taccatggac gcagcttgag agagtgcttc tttaaaggac agcttatctc tagcaataat
     1981 cgcttgaatc ggcgcgcctc ctattccgtc actgaaatca tgccataagc ctttgtcctt
     2041 gcctttcaag cttacaatta agccaccaga ataacgtaac tctctggagt tttgtgtttt
     2101 tggctcaccc cagattgttt tataggtagt ttctgggtcg gccatcagag cttcattaat
     2161 aattctggca tccaaaaatt gtgcagattg ttggattttt tgtaagaccg gattggttga
     2221 ttgcaatgat ttttgggtga tcgtttctgg cttaaaatcg tagttatttt ctaattcctt
     2281 attaattgta tttacagatg acacattcaa tggatattct tgcaaagcta attgataacc
     2341 gctgcccttt tcaatttgct tcatatgact ggaaagagat aaccagttct ttttccaggc
     2401 tgatatttct tttgcagaat tagagtttgt gacgctaggg aactgcattg atagtgtgtg
     2461 tacgacttca tttagctcct taatttcacg caattttatt tggtggttgc tagcctgggc
     2521 taacagcttt tctttgttgc ctttttcata ggataaaatt gaatcaaggt actttgagcc
     2581 ttttaattga tgagccaatg catcacgttt tgatgactga ctgattgctg ctgatatcag
     2641 cttgttattt tgtacattgc catcagcttt attaaggtgc gcctgctgcc aaagcctgta
     2701 agtttgcata ctggcttttt tgtaagtcag gaaacattga tagtcttttt tctcaggtga
     2761 agaaatatta gataatcgtt cctttctgtc ggtaactcgt agatatttac taagggtttt
     2821 agattcaata tttgcttctt taataaaggg ataatggcca gtgatatcat ttttaatctc
     2881 attaattact gcaagacgtg ttttgaaatt agacttactt gccgtaaatt gcttgatgtt
     2941 ttcccgatac tgctgttttt ctgtttgtac tgctaacttt gccaaatcca gcttgaaata
     3001 agaggctatt tcaggcatgg ctttgttttg catcaattcg tgagctaact cattgcggcg
     3061 ggtgagtaac tcaatagatt ttggattact actcttccct tcactgtttc ccgcttcttt
     3121 taaacttgct gaccaatttt ctctaagctc aagctgtaat gctttatagg ctttgatgtt
     3181 atcgaacgcc aatcttccct cagcacttaa tccatttcga aacaatttat cgctatgcaa
     3241 acgcgcatct ttatgtatgg attgccataa tgattcaggc ttttgattca attcttttgc
     3301 atgggttaaa acaaaagaat gtgataactt ggattctctt ttaatttgga ctgcaagcct
     3361 ttctttctct tcgccttgtg cgtttaaata aagcgaaata ttggtacgta ttaagtccct
     3421 tgttgcctgc ttgccaagtg attccaggcg catctgtgct tttttaaatt ccttgccata
     3481 ttcatgctgt ggcaatccaa ttcggtttag ttctccgatg gaaaaatgct gcaaataagg
     3541 tttataacgc tccagatccc tactaattac aaaagctact gcattgcgtt tagcggactg
     3601 ttgttgcaga gcttgtttat tctgatttga aattccatta tccaacgatt gggcattgat
     3661 atgctgactt cgtttaagga tttgttggtt taaaaacacg tactgcctga cagtagaaaa
     3721 atcctgcctt tctgatttgg gtagtgaatg taataacttc gaggtttgga aacgcagggc
     3781 ttcacgccga taggtactaa caccaaaacc caagcgttct ttggcaaggc gatacaattt
     3841 gggatcattc acaatggcaa atgctaattt tgccgctttt cgatcttccc cttgttgttt
     3901 gcattgaaca taggtttcta ctgtctgttc caagcccttc cagtcctgtt ctttcgataa
     3961 ttttttttca atggcatttt gtaagactat gctgtttttg tgctctttaa aacgtgcttc
     4021 atgagctttc agagtatctg aatcaaccat atccattgag gagtacttgt cactgttatt
     4081 ggtggtaatg gctttaacca gatgatcttt atcgtccgtg actactatca tttcccaaat
     4141 accacgggtt gtttccacgt aatagttttg gatggttgat gcaaatcggt tttggctttc
     4201 aataaggcca agacctcttt ttttatcttt tccctgggta gcataggtcg tgagtacata
     4261 gccatgatca agatgtcgta gttcttttgc tccgtgaggt aagtgtaggg tttgattgtc
     4321 ttccgttatg attgttattc cctcattgtt gatttgtctg atggttgcca attcagagtt
     4381 gcgaatacca ttgtgttcag aattacgttt ccattgaatt tgatcaccgg ccattagact
     4441 caattgttct cgccgatata cttcaacagg ccgttccaaa tctttgtttt cagttctaaa
     4501 ttcaggtagt gaggacaatt taaattttat ggcttgacca ttttcatgct taagaatcag
     4561 tgtattatta tgcttgtttt tgttagtgac ctgcccaaca gttaaataat ctcctgcttt
     4621 gatgttatac cgctgaatag tatgattaaa acgaatgata tcgttttgac tataataagc
     4681 tgcatttctg agcttaattc cagttaaatt gcgttctttt aatatgggtt gcagatgctc
     4741 ttgtccggtt agtgtccctt cttgaattaa agcgtttcgt aaaatctgag ttatatcctg
     4801 gcgatttctg tgcgttgggg caaagcatag tgtgttttgg cgttcttctg gtgttaatga
     4861 caaccattta cttgaaatat aatcaacccg ttttaaataa gtatcaagcg tttccacatg
     4921 agttaactta tcaacggcat catagatttc acctctacca gcatgaattg ctgattccct
     4981 taattctgag tctttctggc gaagattatc cgtcatggca acggttttaa tcccataatc
     5041 ttgaatgaca gagaataatt tcccgctgga tggggaagga agctgggctt tatcgccaat
     5101 gatctttaac tgggtattaa actgttctgc cagtttgatg attttatgtc cttgtggatt
     5161 agataacata gaggcttcgt ccaccacaaa tatggtttta cttaagtcct gtttctgatt
     5221 ttgcaatctc cctaactctc ttgcaaaggt agaagcattt aaccctcctt tatggcgcag
     5281 ttcattggcg gctgccgagc ctgcggtaat accacgaatt tcatagccct gaagagatgc
     5341 taattcacga gtcaaacgta gcatcgtggt tttaccagtt cctgcatacc cttgaatggc
     5401 aataaatcgg tctgttgagg ttaggaaggt tgtcatagct tttttctgtg aaggggataa
     5461 agcaaatgcc atctcctgtt cttttgcttt tataaactca ctgacttttt gtttcgaaca
     5521 aatagcagaa acagcacctt taccattttc aatacgctgg atggcctcgc tctcaagtgt
     5581 tagttgccag ggcgttgtca ataaaggctt ttgtgtgaag gggtgtcttg ccgtatacag
     5641 gttttgattt gcaatgtttt catcaatggc tttattaatt aaggtttcat cgattatctt
     5701 attgctggcg attacatatt ttagcgcttc ttttttaagt tccctttcct caaacaccgc
     5761 atgctgttga ctgattgatt caatggccac atacacagac tctctggcat gagtcatttc
     5821 gatgttatcc ttaccataaa agcgctcaac aatgttttcc tttaaagttt gaaagagatt
     5881 tttctgaggc ttatacgatg aagccactag ttgatgtgga tcaaagccca tttcctggca
     5941 ttctttgatg atgtcttctt tccatttctc ataatcaatg atttctttat ccaattttgt
     6001 tttttgagcc gcaatcgagg atgctttagc accactccag cctttttcgt caagtaatgc
     6061 ttcgatggat tcccttcttt ttgaaaaatg agttaatacc tgctcaggtg caccctggat
     6121 ttcccacaaa ccatcaccat cagatgccag ctgatagcca agttctttgg ttaaattcgc
     6181 taatttgtta cgaaatttaa gcccgccata gatatggtgc ttcatgattt gttcaaccac
     6241 gcctttttga ccatccatgt cactggcaag cgagcgccac ttcccatctg ggcagtgggt
     6301 catattttga actacaaggt gcgtatgtga attaggatca ttggcacgag agcttggttg
     6361 acgaaatgct gcaatgacta aatttctggt atcaatatat tcaattttgc cgtcaacaag
     6421 ttgcctggct tgtgcaaact ccttttccat ttcttcaacg aaccattcta cggccttatc
     6481 aaacacctct ccaagccttg gatcagcacc actttcaagt aaaattgaaa aagactttgg
     6541 ggcagtaagg gtcatatcaa aaccggggcg atgatgaata ccatccttat cgattcgacc
     6601 gagttgagta ccatcaggta atatgccttt cagaagggca atcatctgtt ctttttctac
     6661 tgcttgcccg tggatcccaa gaattttcgc tccatctcct aaccaacgaa cacttttaga
     6721 atcgttttca taataattga cgacatctag atagtaggct gcggctccat cggcactgtc
     6781 taatggctga agacttaaca tgcttacccc ccaatttgtt cttataaaaa ctcaacaagc
     6841 tctttttctt cctcaagctt tttttctctt gcaattggtt ttgtgatatc tttttttgtt
     6901 gaagttgcag cctctaaagc atcatcatgg gatgcggcaa ggatgggatc gctgtaggtg
     6961 tccaccaatt gctctacttt ttgacgaagt ggatcaggat caatgtctcg tgcaataaat
     7021 tcttcatgaa tgagaggata atcaacgtaa ttaagcttta tttttgccgc aggaaattcc
     7081 cctttgacgc gcaaatatcc ttgtagatca ttgattacct gaatttgggt tggtaaaacc
     7141 aattgttttt ctttagtttg acgagaaact gaaatcccat cacgcatggt gtttgcccca
     7201 taactgatgg actcattcac ctcttcaatt tcgtatgtac caatattgcg ggaggcgtgc
     7261 tccgcatcag tatgttcggg taagcgtagg aacaatcgag tcgaacacag atttgaaagg
     7321 gacgcggcac catcgttacc ataaatggta cgcaactggg atgcaccgtg atgacctaat
     7381 aagaagcaac caccaaattt gcgactttct gatttaactg cggctaaaaa aggtaaggca
     7441 tgcaaggtag ccagctcatc caaaataaac caaacacggc ggttatcatc aggcgctaaa
     7501 cttaagagtt ctttggtagc agtattaatc caggccgaaa ttagtggtct gatggttggg
     7561 tgtttgcgcc catcagaact gacaaacaaa caggaattat tctgctcatt caaaatccag
     7621 tcgcgaatcg aaaagatgtc cccatcatct ttcaaataca gtaaggattt taaataagta
     7681 gccattacgg ttttcacatt taaagcagtc ttttgtactt tatcggatac cagagattct
     7741 gattcagtat gacgcagtaa atgatgaatt cgacctaagt ctgctgtgag caaatattgc
     7801 aataacatca gattggagcg tttaggatca ttttttagct cattggcagt cgagacgaaa
     7861 atcatacgcg cggctttaat ccaaaaggga tccatggtgt tattgatggg catcggcatg
     7921 atggcctcag caagtgcttc aagatctgct ttgtcttcgc attctgccca gacattccag
     7981 gaatggcctc tcgtatctaa gggatttaaa attacatctt gatgttcacg ataaaaacgg
     8041 gataaataag ttcctccttt atcataaata atcgcgcgct cacccctagc ttgtattgtc
     8101 gtcaatagat cacgaatagc aacagtttta ccagatccgg tggttccgac tatttgtata
     8161 tgctgggttt cagaaccaaa gggcagagat acaccaccaa tccgataagg agaaatcctg
     8221 cttgtctttt tgatgcactt ttgtaacgtg ttttcatcaa ctaactcact gccgcgaatg
     8281 atcttagctt tactttgatt ttcccctctt ttttgcaacc acttaaccat gacaattaac
     8341 atcatgacag aggcaaatag tgcctctatc atgctcttaa gtagagcttg ttttaattga
     8401 ttaacaatgc cgatgaccat gggagctttt acaatttgcg ctgaatacac tggataagat
     8461 tcacccgttg gcatgcgaac cacttgcttg gctttgctct gtattaaagt cattgcctgg
     8521 gctttaaccc attgctggcc aatataacgt tgatacgcac tggtattaaa ataaaaagcg
     8581 atgactgaga aaataaaaaa aactatcagc atagcaaggc ttactttctt acctacctgt
     8641 gtgaacatgc ggacattgtg caaaaagatt tgaccgccgc gaataaataa ttttgtagga
     8701 tttaccgcca tcattgactc cgaatcgata taaaaaaagt gaaaatcagg ctacaatgaa
     8761 taaaaaggag aaaatccaat ggagaagaac tataaagggc aagtgatgat aagctcactt
     8821 gatgaattgt cccctgaaca aagaaaaaaa ctgcgtttgg catcaatggg aatcggttct
     8881 cttgttcttt taattgtggt gttactattt ctttgttttg gtgatttccc aagtaacaaa
     8941 gactccttat ttttttactg caccgattgg ctcttattcc ttgtgtcacc tttagcggct
     9001 tttgggcttg gtgcctcttt tggctttgat ttgatgctct cagatctagg ctttgcattt
     9061 tcttttggat cctacggtgg ttcatcacta aaccagccgt tttcctcaca tgagtcgccc
     9121 aattaccatc gttcaaacca ctacggctct tctgtgagca taaacccaag ctcaggtcgg
     9181 ccgatgtcag gaagcagtgg tatggatacc tctggaaacc cttatggaac ccgatcctgg
     9241 taagcatcat tttacgtcct tgtttgcctg ggcaacgatg acatcctgca tgacttgcga
     9301 cccgtcttgc actatgccct caacattctt ggacatctct gacttggcgc gtgcaaaatc
     9361 cttatcaact gcactaaccg aatctttatt gacgcttcct gcattgatgt tctttgctcg
     9421 gtcattgaac gtattggtat aatccccaag ggcatgatca acccccgcac tacctgcaat
     9481 acgactttta aatccttcat ttttttggtg cacttgactt tcgttaatag gatgattttt
     9541 ttgaaattgg ctgattgatt gcgcggtttt ttcaccaaca aattgtcttg cataagactg
     9601 agctaattca ggatcgtgac gaattagcca ttctgcctgt tgcaacccaa tagtgccctg
     9661 actattaggt gcacgatgat gtgataacca atcaataaat cctgtttgag catcaagatt
     9721 aatagatgct gtttgttcgc tggaagtagt tgcttgtcgg tgataactct ctgattcgga
     9781 aaaactcgcc acagcttcat ctcgatactg catagattta tcaaagcctg ctgaaaagtt
     9841 attagccaag cgtccactaa aatcatcaga agtgcggaaa tgatcgtcct tggtggcttg
     9901 tcttgcctca ttcaccacat ctgtaaaatg gtgttcctca ctaaagcgcc gcgcttcatt
     9961 catcaagcgt gcgtcttgat gggattctga tttgtcccgt tcatgacgca catttccccc
    10021 aatctcaata ttaccctttg ccccaggtac tgcagaaccg gttccaaaac caaacgatcc
    10081 tcctaaatta atactggcag cacttaaaac tttagtcgcg tcatcccgag aaatattatg
    10141 atcgtgagca aaggtatcaa tgagattgga tatttttgat gccgccgcat taaagcctgc
    10201 tgaagtggat atttggtgtc cctgcccact ttgttcccca tgtgattgcg tttcaccaag
    10261 ctctgccagg cttctgagtg atgccgcata gtgctctgat gaagcaattg atttattgaa
    10321 cccagcactt ttagcactgt cggataattc agaaaatgca gatcgcgtgc tttctcctaa
    10381 ttgaatatta agcggggtat gcgaggtcgc agttgccatc gtcagtgttt cactgccatc
    10441 acgcgatatt cgcgcaattg aaccactatc aaggcttgtt tcaataccac ccaatgatat
    10501 tctggcgttt tgatcgttat gaaaagaact gctattaaac ccattatgat tagcaaaact
    10561 ggtatttccg tagctgtaat ttcccatttg tgcttcttct gccgcagagg aggcggcatt
    10621 ttgaacaata cctgttggtt gttgcgtgag tgatacaaaa ctacccacgt gtcgactgat
    10681 taacatataa ctcaatgtgg ttgtcgccaa gactaagtag cctcccaagg cagccatatt
    10741 ttcatagact tgattcatgc ctgtttggta ggccaaagta gtcgcccctg ttgacacaaa
    10801 ggacaatgaa aactgcgcca aaatagtcat gatgtagtta ataacagtaa acatcggtgc
    10861 ccatagggca agccagacat acagtgttgc gtaaaattga aaaaaagaaa tgccggaggg
    10921 aaaggggaaa aaaaacaaga caaaaataaa acaccctata acaatgcaaa ataaaagact
    10981 ttgcaacatg ggcaaccaat aggcggacat tagtccaatg ttgctgagtg ccgcgggtat
    11041 tttatcctgt gctcgactgg ctgaaaaact gtctatggcc gcttttgaat tcacacgtgc
    11101 gcccatagaa aacacgccat cgcgaatggc gttggccatc atattttgcc gaataagtgc
    11161 tgcggcatca cctgacatgg cggttaaatg attataagct tgaggtaagt atttttcgaa
    11221 attagctagg gcatctgaat gattgccaaa agcaaaacct gcatacttag atttggcatc
    11281 ttcaatcacg ccactccagg cactgtctaa taaactagcg gcttccatac acgatttaaa
    11341 tgaaccatcg taaataaacc cacgagcaac agaggcattg gatttaacca attcccaaat
    11401 atctcttgct tgattaatgt cttttaagcc atatcgtcca tacatcacat catagaacac
    11461 gcattgctgc atgaactttt gcatgtttgc attaaacaac ggatcggtta tttttatatt
    11521 acttgctgat aaaaagagct tcgcacccat gatcatgcct gtttggctgt aatccatatc
    11581 gttaggtgaa tgaaagaaga attcaaaaag ccgggttatt ttatcactta gctgactgct
    11641 taatcctcct acaacaccca aaactaaggg cacgttatca accgttaaat ctggtcgcag
    11701 aggatcagtt ctatcgtgga tttgcaaatt gcaggtaggt gttagaataa atgaaataac
    11761 aaaagcatat tttaaaataa agaagaacat actgcgcaca tcacgtttta caataaattg
    11821 gccaacagtc caaaccccac caataatcag tgctgttcta aaaatcccaa gtattccatc
    11881 atagtgaaac atagctgcgg tggcgttata aaacgcctta agtaactcac caccggtaat
    11941 cgtataaatt tgcatcgtaa cagccatttc actcacctca tttgattagc ccaactcaaa
    12001 gactgagtga gctctgatga aaattcgcct acaagcattt gctcgatggt ttgagtttct
    12061 tgaatcatct gcatcgccat gctcattcgc tgatgcgcgc taccttccat ttgaaccaag
    12121 tcgcgacgag cctgggtgag atcagtttga aactctttca ttattgcttc tggatattgc
    12181 agagctcctg ctgcttgttt aacgagttgc aggttttcat tcaaatattg aaataaaata
    12241 tcgatggcaa tggcttctga gtaacgtgta gtgtcaatca ctgcactgtc cttcataaag
    12301 gccatttgaa cgctgaccat tttcatcacc ggatattgtg tggactcaat taatccttta
    12361 gcggcctcac tttgctccac attgtcttta atcttgcttg ccatatccaa aatcagatca
    12421 cgaacttttg attcaagacc atgactgata tcaatgtttt tactggttaa ggtgggttgt
    12481 aagcatttga ccttttcatc acattgatag atttttacct cgccaccgcg taaaagagcg
    12541 ctgattaaat tgttattact tactaaggaa gacaggcgtt ggacatgcac cccttctgca
    12601 tcgcgagtca gaattaaggt gcctgataag gtcatcatga gttgagctaa ttcgatatca
    12661 tctgcaaata agccgttttt ctttaacgca ttccacgcca cattggtatt atcaactacc
    12721 tgctcattgc tgctgttttc attaatcact ctgtcgtaat cataattact ggagccttta
    12781 ccacagcctt gtcgtgcctc ggcccagtct gaaaagagat ttttggattg accgatggat
    12841 tggcaggcat gttgttgtgc aatacgggtg cgtggggcta aacccagtac ggcactttcc
    12901 gcggttgcgc acgagctgat attggtttgg ttcacccagg tagccacgtt ttgtaatttg
    12961 gttgccacat tacctaaaag aggagttgct tgctctaggg ccaattggaa ggcaagacct
    13021 tgtgagttgt tcaatatatt cttggtaaaa tcgacaaatt ctttaccttt gatgtgagaa
    13081 aacccaccta acgtggcatc tatgccgcca cagcctgcgg acactttagg ccagctcaaa
    13141 cgcatgattt gcagattacg tacgctgctt ctagcgtata aattaccgcc agtataaaaa
    13201 ccagcacgct gcgcttggta agcatgcggc tctgttacat tcgcgcccat attaagagct
    13261 ttaaaataac cagttaaatc ctcacctatt gatttgctgt acacttgtcc gatgccaagt
    13321 gcaccgatta acccacaaga cagaagaatt tttttcataa aggcacctct ttgtcatgta
    13381 tctggctaat ttttagtaac cgattggtta gttcgccttc atcaatagtg ccttcagtaa
    13441 caagactcgc cacttggctc tttgggttaa tcaaaaaaag agaaggataa acactgactt
    13501 gaaatacctt cgtttgtcct tcattcatca ccgcatcagg aaaggatggt aaaaagcctc
    13561 catcaacaga aatagcaact acgctaaagc catagtgttg actcaagcgt ttgagcgtgg
    13621 gtgcaaagcg ttgacaatga ggacaactcg aactgaaaaa aaagaaaaat ccttgatgtt
    13681 tggcaagggc atgaagcggt gcattatcaa tatcggcgta taggcttgat gaaaataacg
    13741 ttcctaatat tagaaaaata gctaatttat tgcgcattgt aatctcccga ttgaaaagcg
    13801 ttgcttgcca ttaaaatgtt ttccttcaat tccgactggc tgactaagcc ataagcaact
    13861 ggcatggttt ttttagatac tggattaaga gcaaaaaccg caggaatgtg tttaacaccc
    13921 atctgagttg cctgaccgga atcaactcgt gagttaggta acatcgggga gatgcgattg
    13981 gtcatggcaa ttggaataac agtcatgtga taactgttcg caaaatcact cactacttgg
    14041 gccatgcgct cgctaaatgg ttcaccacct tcatagaaat acaacaaacc agatgtttga
    14101 ctaatttgag taatcgcctt ttccttttgc atgctgtttt gctggttgta gatatcacgt
    14161 gctgcactac tgctcggatt aataagactt tcatcctgat cggggtttat tagcagatcg
    14221 agcatccatt tctcagaaaa cagattagcc tgtgccacaa tcaatcgttg taatcgtctt
    14281 gcacgagcaa tattttcagg agttggattt aaaatagctt gtgcgcgagc ctgttgcact
    14341 ctattaccaa tcaatttcca tgttttatca ggatcggcag gtttacttac atgtgaaggt
    14401 gccactgact cggtttgtgg tttattcttg acttcctctt tgggatcctt atgccaatac
    14461 caaccttcct catgctcatt aagataaggc tgctcggcgt acaaaaaagg actcacgcta
    14521 accaacaaaa caaaaaacca tctagccatt gatgtctcct tgattgtaaa attgattaag
    14581 gcgctggctt attccattgg cagtctgttg gtaatccggt tgtttttgtt tattttttac
    14641 ctcctcataa aattctgaaa aatcaatggc ttcgaagtga attaattgca attgctcagg
    14701 cgtaataccc gagcaatttg aatatttatt ttggccaaaa ccaatatgta actgatcacg
    14761 tcttccttgt acttgaacca tacgggctaa tttagattgg aagacacagt aagtctggtg
    14821 atgatcgaca cagaccgagc caatagggag ttttttgcgt ttaaaacaat attcacccgt
    14881 aggtactacc agtttgtttt ctcgagcttt tcccagtttt ttctcagcat cactacactg
    14941 tgccagctca agatccagac cccacccctt atcactgcaa caatttttaa aatggagcat
    15001 tgttgttgaa cattcaagca attgaccttt gaaaataaaa ttggcattgc catcaaagtc
    15061 ttttgaggca tccgaggcgg cggataaggt ggacattgct tttttaaaat cgtcttcatt
    15121 ggctggggtt agttcatgtt tcgcgcaatt accatccaaa caaaaagcat cctcgccaca
    15181 caacagttgg ttgtcagtac attggtttat cgggcattga tacgtttgct gatgggttgt
    15241 gcaaagtcct gcttcttctt tcacacaagt tgaagcagtc tgctcacatc cttgattaac
    15301 taggttatcg cacgtatttt gctcctgact accaccacag gtgtaggatg ctctctcttt
    15361 ccagcaaggg cgggtcaatg gaacatcgcc aataatccgt gtttgattgg gctcagtgca
    15421 tgttagaggc tcagtaatat gacacaaccc ctctttttct ttgttttgca aatgttggca
    15481 ttggtcgtcc caatactctt tgctttcata aatttctacc gtcatcgaaa cggagtgaac
    15541 atagggcact gtgcagcgac cgaatcgaca attgccaaca gacaatgata gggtgggatt
    15601 ggcgcatgta gctgtttgct caacaaccac taatttcttt tcatcaagga aactgacctt
    15661 caccatggcg caattggcag gcaatagaat cgctggtgct ggtgctaaat ccttataaat
    15721 cgtatagcaa ggagttttgc cttgttggca ggtatccgct tgtgccagat taactgagac
    15781 tttaaaaggg gtattcctca tacctccttt tcgcaggtat agactgtaac tttctgtttt
    15841 gtagggggaa acatctaacc ttaagtgtct tgcacaatgc aatacaccta ataccttgct
    15901 agacaggcat gttttttcct gccatgagta atggcattct ttgggtttta aagagcaggt
    15961 ggttttatcg gtattttgac catgcataat cgcatctcca ttttccgcga tttgatttaa
    16021 cttttgcatt gattccgagg caggatttac ttgatacaag gggcgatgat taaaactttc
    16081 gcctactgcc tttcctgcat cattctgagc cacggcagtt tgcgaatcgg cttcaagacc
    16141 tgtgcttgcc tgcgtgacgc cactataata atgttcttgc ggcagattgg cttgatagcc
    16201 aggaaactgg ctcagatcca gtgcttttaa taaatcaatg gattgattgg tatggcttgt
    16261 tccggtctga acgccttctt gatacgcctg attcaaatca agtgcaaaaa cctgtgaaca
    16321 aaggattaaa aaaactaaaa cgaagccttt cattagatta attcccctct taaccgaaga
    16381 atcatttgat ccaacacggg gtcttctgac tgctcatctc ccctcatttt ttccaaagca
    16441 tagtccagcg atacatcacc ataaagactg tcgtaactca ccggtttgca atccatattg
    16501 gcaggacaag caggggtgtc tttcacataa accactgcag gaaccatagc gatatcaaag
    16561 gttttaaaaa gaataggatc aagctgcatc ccaattcctg tttttttact gagcgtttga
    16621 atagccgtca tcgtttcttt aaacgaatta tgaattagtc cccgaatcac aggtgttgct
    16681 ccactttttt tacattgtaa aagccaggct tttagacttt tttctggcat tgaaaacgat
    16741 aagaacacga gtacctgact gacttttttt aagtgggtta caatgggctc ctgtgttttt
    16801 atgacttgat gcatctcgcc ggtaaattct gcaatttgac ccgatttttc tgaagtctca
    16861 agagacttac tgatttgctt tatttcattt gtaaaagcat tggcgcgatt ttttgtattc
    16921 gcctcaatag acgcaatata ggcttcatct tctgcaagtg caggaaatga caacaaaaat
    16981 aatgcaacca agaaagaagt gttcattcgt attgttttca tcacatctcc taatcgttaa
    17041 aaggcgcaac aattgcgttt gcgccagatc agatacccaa aatcctcgcc cttaaacgga
    17101 tattcgtgcc ctgcctccca tagggtcgta gcacgcccaa ggggataaca ggcattagta
    17161 gtcggtaaag gattgaccat ttgatagcgg taacgtgatt tgggcaaaat aggggaataa
    17221 tgagtatgac aaatggattt tgcgctgctt gtcgaatcgt caagtcctac tgtgtcttcc
    17281 aatagtccct ggcggtgcag tttaaaattc atacgctcag acagtaagac tgatgcctga
    17341 accccgccaa catgttcttg cacatgcccc gttaaagggt acatgcttcc ctgagctcct
    17401 gcgcaccaaa agagtttgtc aatggcactg cccaataaag cggtacttga atcagcagcg
    17461 caagccgctt gtgcgggtaa actaccaaac aataccgcct caggattaat gacgaaaccc
    17521 agctcatcgt cattccacat cacatccaat tcactcggat aagcaatgtc aaagtctcct
    17581 ttttcaacac aaatcccatc agttaacaca ttcaaccaat acatcaatgg gaacttgtac
    17641 cagtgcacgt ggtaaaatga ttgattggat aaattactat ctgtttccac cgcacctttg
    17701 cggtaatact tgccaaaatt taaagaaatt ccccctaaat taactaagca aaatggggtt
    17761 ctggttacat ccactaagtt aacaggctcc caataaccca ttgttaatcc caatctcggt
    17821 ataggatttc ctgggcattc acaaacaggc agtttgggat ttttagtgtc tggcaaatga
    17881 ttggaggaaa cgagattagc ttgccctaaa ctgaacggga acaaacacga ccaacagaca
    17941 tcagtaatgg gatttacaaa acgaccatga caagatcctg cttgggtcaa cccagaagct
    18001 aaaaaagcaa taagaataaa aaaggctttc atacagtcac ctcattaatt tgcaaccgct
    18061 tccctgcttg aaaaacccgc gccggcaaat actttatttg aaatttggca accaaaaatc
    18121 catttaaatc aaaataaata gcttgcttta attgattcgc aacttccttt acagagcctg
    18181 aggtgaggat taattttact ttttggtgct gcgttaattc ctgggtaacc catgcaacct
    18241 gtgcctcatc atccccgtca aaaaacagga gagtgctact caagcccact ctatccaggg
    18301 ggttaattac agtgccttgt ttaacaatca cttggccgtg actgtccttc acatcgtaag
    18361 gcacagcaag tgatggatta aaaaaccacg ttctattgtc ttctgctctc ggcaaacgaa
    18421 taggtgttgg gcgtgccaca tgttctttta cccgtttttt aaactcagat tgtacggtct
    18481 tccattcccc attaacctgc atccccttca ttttttgctg gatgtactct aaaaaatcta
    18541 tttcagcaat ttcaaaggtt ttgccaaaga caccatagtc tttagcctct gcaaaaggga
    18601 gatagagcat gcaaataatc aacagaagct ttttcacgat ttatgctcca atttgatcat
    18661 cgtcatgagg gtggctgtat aatcagtgct gcctttaatg accgcttctc ttggtaataa
    18721 caccaacgat ttggaatggg acaagtcttg taacgctgat tccaaacgat gcgaaaatgc
    18781 tttaatttct gcttctttct caaggttgga atgatttttt tgcgcttcct ctttgataaa
    18841 ttgggaggtg attgacaaga tatcaatggt tgcaatatct ggccgcctca aagcactaaa
    18901 aaaaatccca ttaactaaga tactggtgat gagtgcggtc aaaagagata cttgagttgt
    18961 cttaaacata gccatacacc tccagggcaa ctttggaaat ggcatcagcc aatggcaaac
    19021 ctgttgccat taatcgtttc aaggcctcga cttcaggacc acgtgtagtg tttaagacgc
    19081 gtgaaaatgg atcgacaatc aaacgatgca ctgttgaatt ctcttcacac atcattaaaa
    19141 actcagagta ttcattagtt attttaaggc ttcggattaa acgctcgcca taagcatcca
    19201 actgattact cgtttttaaa gtgtcaatcg tacttttgga ggcacctaaa aagattttat
    19261 aataggcgtt gtcgtaacac attttggcag ttggattgtt ttcataatca tctaagccat
    19321 gagtcactgt aataaaatta ccaccgtatt taggcgcacg cctatagccc tcgttaacaa
    19381 agttagcact gccacccgta ccattgtctg aaagtaattc gtaggattca tcaatcaagc
    19441 agcttttggg aatgtgcttt ggcgtgtaat acatcgcatt ggtgatttga tacattaaga
    19501 ttttcaatac aatgcgctgc aaatccttat ttccatcaag accacccaac tctaaaatga
    19561 ctaaatttct tttaaaatcg agcgaacaag gcggttcaaa ataactgcca taacgaccgt
    19621 ttttagtata gctatacaac aggcgcccaa tattttgaga aataggatcg ttactacttt
    19681 ctaagtgatg agcaataaga gtaatagttg tagcattgtg atgttgtcgc cacatcgcct
    19741 caatggcttt ctcaatatga cttcgttctt catcactgac gttacctgat ggtcttgcca
    19801 tcatacctac aagttgtatt agctgattgt gaacgtcttt aaatgcagta tcatttttgg
    19861 cttgtgcatc aattgtagaa aagaggttta ggctaatttc agtgtcatga ttaaaatcaa
    19921 taagctgtcc gttcaacatc tcacaagatt tagcgtatga accgcccaca tcaatgatat
    19981 aaacaaagcc cccctgggat aacagacttt gaatgtagtc attcattaaa aaggattttc
    20041 cttttccagg aggagctaca atagcgacat tatagtttct gactacgttg tcaaaaaaat
    20101 aaatagcggt aaattgccct cgtctttgtg gatagacctg agcaaatcgt cctgcccctt
    20161 tccattcccc ctggataggt aataagttaa ccgtattaaa actggttaaa ggtcttaccc
    20221 gtccaaatga tttgtaatca tcaaacatac cttcagcagg tgcaaagggc atggaggcta
    20281 gaagtgtttg taactgcaaa cctgttggcg tgcgtaaatc aaattggtta ccacgaaata
    20341 catccttggc acgtcgtaca tcgcgacgga cattggcagg tgttgtaaac aataaaacgt
    20401 tataatagga atcaaccaaa gaatcatcgc cactgagccg ctctcttaac tcctcaattt
    20461 ctgcgtattt tttgttaaaa tttttgaccc atttggcaat aaaaggttgt ttggcatttt
    20521 gcccttcatc agcatacttg gcatctaatg ccgccatcac cttgtcttta tctttaattc
    20581 gtattgcaaa actaaaaaga aagggacaac ttatttgcaa agaggtatta aacatttttc
    20641 caaataaatc acccagttgc cattgcacag gggatttagg atattgccta acggacaagc
    20701 atttaatcac cctcgtatct tcttctcgct caatctctat ttgattggat ttaacccgca
    20761 tactcgcttc aatgtcagtc agttgaaaac gaatttcagt attggtatcg taattggttt
    20821 tttcttcata caagctgtcg gttggaaaga gccactcccg ctttaagcta atcacttctt
    20881 caatagacag ttctttacaa ataatattga ctgactttag attgttgctg atgtcttcgc
    20941 gcaaactacc aagcaacaag cgatcgtctt ctatggattt gcgcggaatt gaaatacata
    21001 aaaataaacg aaaatcacgc aagacaaaat tgctttcttt agttaatgag ctgtacaccc
    21061 ctttgcggta aaattcagct cgtcggtgtg ccagggcttt taaaacctca ctgccttgat
    21121 tgcgttgatg ttccatttca tcaatcatgt ggccaatttt aggcgatgcc caaaacaaag
    21181 catgaaaaca actttccggt ggcaaaatat ccgttaatat actggtgaga atagtcactg
    21241 tttcttcact ggcaccaatc agtgtcgagc attcaaacat catatgagat gcctgtttgt
    21301 aaggataaat atcagcttct gaatcgtaaa cctcgtcagg taaataaggt gctaagcaat
    21361 aagtatccaa cagcgcttcg atttgctttt tatgatgcgc atcagtcatg ccaagctctt
    21421 ctcgcaatgt ttccaatagc gagcctgttg catgacttaa aatagtgctt gtttttttaa
    21481 ctgcaagacg cgcttttttt atagttgaca acataatggc tccccttatt ttaaagccaa
    21541 ttgtgactgc tgcatccact gtccttcctt aacaacgctg tacactcgat tggcttgatg
    21601 ataattgcca tcagtgtctt cataaggtgc tatccaaaga ggcatcaccc cttctccata
    21661 acgcacaggc tgaccaggtc gcccatccat caccaaagga tttcggctgg gggttacagc
    21721 cagggatttg cccttaccat tcaccatgtc ataaacctga tccatacgtt tgcacgttcc
    21781 tccattggga gctgggcaat caaagtcctc attcaatttg gcgcaaccgg tcaaaacaac
    21841 acagggcaac agtaataaag tagttaaaat tttcatgatc tatatatcct tagaaagagt
    21901 ggtttttata gaaaaattgg ttggcagctt tttcttcagc agtctcagac tcggcagggg
    21961 cttcaaaagc tactttggaa ttttggcttt cttctggttg ttcaggggca ttagtgtccg
    22021 ttcccccatc aagccaaaaa cccttaagaa aagttacatc tactgtaacg attggaggca
    22081 attgaataat cgggtggtac atttcagcac gcttgatgtt gtattgcgca tacatttttg
    22141 cagcgcccga tccgccacta gaaacagccg cgagaggaat ttttcctgct tgaatggttg
    22201 atacacttcc tagaggcgag gtgctgtaat cagtggaata ttgctggcca atattgccca
    22261 tgctttccca gaagttcccc caaaaagctt ttttcaccat cggttcatca cgccataccg
    22321 cacgcccacg aattccgttt ttagaatctg aaatgatgcc tgtaacctgg atgtcgatat
    22381 tggtgccatt ggaatgaatg caacttaatc gttcggtacg aaactttcct cgctcactgg
    22441 aaatatcgcc aattacagaa gcacttacca tacagccttt aagtggtgcc ctgtatttat
    22501 tcggcatcac tccatcatcg acaatttgaa atatcataat gtcgggattt gcttggctta
    22561 aaactcccgc agatgcatct gcaccttgta aaacaactgc tttagcaaag gtgttggaga
    22621 agacataatt attgggtgtt ttgattcgtg gcttggattt tttatttaac ttggctgtgt
    22681 attgaataaa tcccgaatcc atctccatgg agttgctcgc tgccgcttca tctgctcggg
    22741 gtaacaaccc attttgaggc tcattgtttt ttaatgtccc atctattggg gtagtacgct
    22801 ccatttgtag cttaactatt tgatgcttta attcagaaag tgtggatttc atttcagata
    22861 gttcatcttt ttgcgaggta atgagcttgc tttgcttgtc tttttcatca cctaatgcct
    22921 tgaggttttt atcaaagagc tctgattttg caagctcagc tcgccaggca ttttgtgttt
    22981 tctcaatcca atgggattca ttagtggcag tagatatcgg gctggctaag ctgtgttttt
    23041 tataagcggg tttatcaacc tctctggatt taactgtctt gtttcccaat aaggcaaagc
    23101 acgcaatcac caacactata acgccgataa caatccctaa aaagatctgt tgttgttttt
    23161 taagttgaat agacatgtta gctaatcctg tacaatcgag tagtttcaaa cggcgctaaa
    23221 tgggttttag caagcgccac ccctttggtt ccaggccaat taaaccagga ttccttggca
    23281 tcaataggct ggttggttac gttttttaac tcaaaaattt caccccgcat acgattgcca
    23341 ttaactgtca ataagcgttt tagttgtgtt ttttgtgctt tatagactga aggatttttg
    23401 ggtattgttt gggtaaaacc ttctggcaaa tacccattaa caagcgctcg taaaaaacca
    23461 accatcacct tttcataact tgatgtatgt ggagcgcggg cacgaattac aggatttctg
    23521 taggatttaa aaacgattgt ttgtgacgca atgggctttg gttctacacg cacgttgtaa
    23581 aaaagacctt tttcagtcag caaattgata gtaaaagctc gtgtgtggta tagaggcgat
    23641 ggcaaaaaag taagaatacc cgtcgtatca tcaattaatt tgcaattcgc cttcccattg
    23701 catgcatcaa ccagggtgtt gccaggtgct tttatttgct gaatgcgatc atcctctaac
    23761 ataagattat tgggttcttt aattgaggca ttaatgaaaa tatcttcata atttctgact
    23821 gtttttattt gagcaccttc tgctgttgct attaataagc ctaagctgaa tatcattgcc
    23881 atttgtttca tgattacacc ttctcaagat tcgttagttt caacaggcca tactcatagg
    23941 aatacctaat caaaaaagtc tcccgtacat cagacatcaa atccgcgcca acaaaacgct
    24001 tcatctgtcc cattactttt acgctcaagt gtttgatatc aatttcaaag gtcgatggat
    24061 aaaacacgct ggataaatgt tcgtgattaa tttgttcgac ttccttaacc aattgcgccc
    24121 tgatgtcccc atacagtgag ggagctacat agcctaagag cgcattaaat tggtaagtgg
    24181 ctgaacttga agtgatatta aaacgaagtt gcgttaagaa attggtcatg tctcttaaat
    24241 aaccttccga aacacctgtt ccagaaagtg taaattcttg agatatttta ggtgggatga
    24301 aatgggtttc atgtttgcta tgagcataat gcaataagac cacaatggta attgattgca
    24361 tgaatgtcat agctagcagc acactactaa tacctatgaa taaaacccta atcttttttt
    24421 ccttcattaa ttctgatttt ttttgttcta ttttcatctg ttaccccaaa aacgctctaa
    24481 tccatgacgg tggtgttgcc cgtaacttag gtgatagaga aaagaaccaa taaaaaaggt
    24541 ggatataaaa atagggacct tcattaccct tcatgcgttt cataagaaat agaaacgcac
    24601 aggttagaat caatgaggta acaaaaaaac cacaaacagc cccaaatacg tagagagtta
    24661 gtgctcctac catgacctca tcgagtgtaa aaataatcaa tcgataaggc tcattaagat
    24721 attggggtgt tttgtatttt ctcggatctt ccatcttaaa cccctgctag cattgcaacg
    24781 acatttagaa aaatagccat cgccccaacg ccgccaagcg ttttaatatt tcgagtaaac
    24841 atcaaggtaa caacagccgc tacagcttct ccgacataaa gataggtttt tatcgaacca
    24901 ttgtaggtat ccttaactac ttcttctgca gcggataaag catccgctgc aaaggttcct
    24961 tgagccatta ttagcaagca aataagtatg aaaatctttc caatgtgagc attaatactg
    25021 cttaacacgt tacttctgaa ctgatttaat aaagacatgt tatccctcct ttttaatggt
    25081 gattgaggat tgattgctgc attgatgggc aaaatttctt tgttggatca gccaaaccat
    25141 cgtgcttttc agattgaatt ttttgataaa tttcataacg gtggatggct actgaattgg
    25201 gtgcattaaa cccaatgcgt acctgattcc ctttacaaca tagaactgtg ataaatatat
    25261 cttctccaat aacaatgctt tcaccgactc ttctagttaa actcaacatc ttcttctcct
    25321 ttttagttaa ctttcatatt taaaatgtgt ttcattttca gtaattgggt tttagccact
    25381 tcagggttat tgtttttggg agctgcatca ggtacaaaaa atgaacttct cttggaacaa
    25441 tttttacaaa aatcaataaa ttggggtaag gttggcggta gttccttatt ttttcgacat
    25501 aaatcaattg ccatatcaag aatgttatcc gcataggtaa atagtccttg ttgccattca
    25561 cctttaacaa aaactaaaaa ctcatcgctt ttaaattgac tacgccaaac ctgtccgtac
    25621 atagctgcaa acctcaaaaa taatcgatca atgcgcttgc gaccttcctc ggtaggcgct
    25681 aaggtcgatg atgttgtcat cggtttcttc ttcaatgcct gatttacatg actcccagaa
    25741 gctgtctcta atgggttgtt tcttataatt tgtttcatgg actgcattat ttgtctcctg
    25801 acacgtatca ctttgaattt catcttccca acaatgttgt gctaaccaat tggcaggata
    25861 tttccaagca gcaacccatt ggccttggct atgctgttgt tgataaaact ggatttgttg
    25921 ttgtaaagcc gaataaattt gtattggcaa ctcaggtgtg agattaagcg cttgaaatgc
    25981 ttcccatgct ttttgctttg atttttttag cgggtacaaa tcccaaaatt tttcgaattg
    26041 tgataataaa aaaatataat aattatcttc ttttagaggt gtgtcggctt ttggagattt
    26101 atctatgccg gcttttagtg ggtctgtctt tgataagcca gtatttataa ggcgttgctg
    26161 atttttagct gggtctagtt gctgtgtctg gtttaggtct agtttatttt ggacggaata
    26221 acccattgta gctaattcgc atcttaaaat taattttaaa ccttcggatt gtggtgtaat
    26281 aagccctgct cgccccaaag cagataaggc acggcgtatt tgcatgcgtg aatagctccc
    26341 gcttttaatg ccttgatgcg gctcaatata aagttgctct gctatcgatt gatagctgat
    26401 tccgcgcttt atgccgacta atcctgtttt gacatccata tatggcctta tccctcttag
    26461 gtaagccagt tgctgaaggt gtggcatccc acataaagca gcaagttctt tgtcattaat
    26521 taaaaaatcc actattcact ctcaatccgt gtacaaattc gttattattt tttttatatg
    26581 gtatatttac tattaactac tacttgagtc gtacgattcg tgtaaataat tattttaatt
    26641 taccactatt tggtgcattg tcaatatata ttttcacaat atgggattaa ttgggttggt
    26701 taatatgaat ataaaagaaa aaattggtca aagaattcaa gaagaacgga tagcaaaagg
    26761 attaactaga aaggcccttg aggagttaac tgatgatctt aaacaatcaa gaattagcaa
    26821 ttgggagcga ggtgatagaa ctccagggcc tgaagaaata aaacaacttg ctagagcact
    26881 agatatttca cctgcttatt tgatgtgcct aaccgatgag aaacgtccaa aaaaaattcc
    26941 aggattaggg tcattggttc cactgctcga tcatcaacaa gcctgcgatc ctaaaggatt
    27001 cattcaaagg gttaggaacg aacataattc agatgaaata agctttatac cgatttcttc
    27061 tgagcttagt actcgtcttg gagaaaatgc atttgctcta aaaatgaaag atgagagtat
    27121 gcaccctgaa ttaagagtta atgatatttt gattattgat cccaattctg aactgcatcc
    27181 aggaagttta gttgcagcac agcttaacga agaaaatgag gttatcgtgc gtagatataa
    27241 acaactaacc ttatcaaaag aatttaatcc atttgaattg cgggccgaaa atgataattg
    27301 ggggaatata aaaattgata aagtaactga cgctataatt atcggcgtta ttgtgtgtgt
    27361 tgtgaggtat acagggagat actaatacat ttaacctaat taattcgatt taccagtgaa
    27421 atgttaacgg atagttctga gattttttat aaaccactca tgttacgcac gtgaacttgc
    27481 agtttgaata aaaagcatta gaattttgcc cttaatttta gggagtgaat tcaatggata
    27541 tgcttgatct ttattgtgat tatttaattt tccaaaacaa atatgcgact gctaccgggt
    27601 tatctgattt ggttgatgga gcatttgctc ataacaaagt aacacgattt cttcgttttg
    27661 aacattttgg ttcaaaatca ctttggcact atgtcaaaaa gccagttagg tcacaagaaa
    27721 cagtaggagg agtattgata cttgatgatt ccatcgaaga gaagccctac tctgatgaga
    27781 atgaagtgaa ttgctggtat tactctcatg ctaaaggggc tgtactcaaa gggataaaca
    27841 tcttgtattg catggttcgt tacgctgact tcagtgtatc cataggatat aaggtgatta
    27901 aaaaagatat cgcttattgt gatattaaaa ccagacaaac cagaagaaaa tcatcagtga
    27961 acaagaatca attatttcaa agcttgattg cacaggctat taccaataag gtgatgtttg
    28021 attatgtgct agctgacaac tggtttggct cgaaggccaa tatggttcac atccataaag
    28081 accttcaaaa gtcgtttatc attggaatca agtctaacct aacattagcc ttatctgaaa
    28141 acgatgccaa aaacgggcgg taccaacaag taagagcatt agaacttgaa gaggatatac
    28201 tggcttaaag gcctagacct ccccgtgagg ctcctgaaaa aggttttcaa aaacgaaaat
    28261 ggctcaacag gaattctcta tcttgtttca aatgacatgc aaagtagtgc cgaacgtctt
    28321 tatgaagtgt accctggcgg attgaagagt atcacaagtc aattaagcaa aatgcaagct
    28381 taaccaagtc cccagctcga atcgtcataa cacagtgcaa tcatatttat gcgtctatta
    28441 ttgcttactg caagctcgaa atgctgaaga taaaaaccca attaaatcac tttgccatta
    28501 agtacaaact aattgttaga gcgaaccaaa ttgcctggca ggggcttaaa aatatggctg
    28561 cttaaaattg atcgtgggta acatgagtat ttgagctaaa accttaaagt aattttctgc
    28621 tacacttaaa atacaaactg tcttccaagg ttaggtatgc gctacctctt tattttgata
    28681 gctcttctat cccaaccact ttttgctaaa ccatccgaag ccatctttgc tggtggttgt
    28741 ttttggtgtg ttgaagccga ttttgataaa gttcctgggg ttttagagac tatttcaggc
    28801 tatgatggtg gtactgagcc taggcctggt tatgcattgg tctcatcggg taaaactgct
    28861 tatgtagagg ttgtgcgtgt tgtctttgat agcgataaag tgacctatca agaattacta
    28921 gatttttatt ggcgtcatat cgacccaaca gtacaaaacc agcaattttg cgatgttggt
    28981 aagcaatatc gcagcgtcat tttctattta aatgacgatc aaaaaaaggc agctttagct
    29041 agtattgatg aaataaaaaa acgatttcct aaggtatata cagaggtaat tccctctaca
    29101 acgttttatc ctgcagaaga atatcatcaa gattattatc aaaaaaatcc aattcgttac
    29161 aaatattacc gttacaattg tggtcgtgat gctcgtatca aggagttatg ggatgaaaag
    29221 tctagttagt tttattgtaa gcgtatttat gctaaatact gccctggctt acacgcctga
    29281 atttaacaaa tccgaaaaac taaaacagct tacgcctcta caataccaag tgactcaaaa
    29341 ggctgctaca gaaaaacctt ttgataatcc ctattggaat aatgaacaag ccggaattta
    29401 tgtggacata gtttcaggag agcctctgtt tagttctctg gataaatacg actcaggaac
    29461 cggttggcct agttttacaa aacccataaa taccgcaaat gttattttaa aacccgcgag
    29521 acactatttg ttttttgtgc aaactgaagt gctttctaaa aatgcgcaat ctcatttagg
    29581 gcatgtattt aatgacggcc caaaacccac cggtgaacga tattgcatga attcggcggc
    29641 attgcgattt atcccaaaag atcaaatgag caaagaaggc tatgaagagt atttaactct
    29701 ttttaaatag attgcttatg aaattcataa agcctgtgca tcgctgtccc tatcactaac
    29761 aaagaaaaga tcattgctaa acttaggaag tggtttggcc aaagcatcat ggctataaaa
    29821 aaaacaaagg cttcagctct ttctattaat cctgggctgt aatgaaaact tttttctgaa
    29881 tcattagtct taaaaatacc cacaacaaga aaactcgtga tacaaagtag tatactcgcc
    29941 atcattaaca aagacccaag accccgctca tttggattaa tagcccataa ggcaaacaca
    30001 acacccacct caaccacgcg atcagtcata atatccaata cactacccca gtcagagcta
    30061 tggttagtta gacgagcaat agtaccatcg agggtatcac aatatccaga cagtaatagt
    30121 aaactaatgg ccagataggg tagatttagc cacagtgcgg gtatcactaa aagacccaat
    30181 acgccagaaa agaaggtgac ttgatttggg gtcactcgct ctatcaacac aggagtaatt
    30241 ggattgacac aaaggcgttg atacagtggc ctaaggtgtt gttctatcat agatttatcc
    30301 taatttattt agccaacgaa cgaaagttcg aattttagaa gaaaatataa acggcttata
    30361 atactctccc gcaactcgct tactaatttc actaaaagtg gggtacgctg caatcgcatc
    30421 ggtaaaagtt cgcaaacttt tccgttcttt aacggctata acccaaggta aaattaattc
    30481 acccgcatga gcgcccacta tgctcacacc caaaatttta gcttttttac cggttatgat
    30541 ttttattttg ccatttacct tattttccgc ttgggctctg tccacctcgg cgaaggcaaa
    30601 ttctgtgatt tttaatgtag gatcttttaa ggcctcactc gccaataggc ccacgtgggc
    30661 taattcgggc tcagtataag tcacccaagg caccgctttg taatctactt ttgcaggcca
    30721 tttaaaagca atatttctca atgcaatacc cgcttgataa ctagccatgt gcgtaaattg
    30781 ataaggccct gtcacatcgc ctatggcata gattttttta ttagtggttt gaagtctggc
    30841 attcgtgttt atccctctgg tggtgtattg aacgcctgct ttttccaaac cgagcccgtc
    30901 cacattagcc acacgacctg ttgataccaa caaatgcgag cctgtgatag taattgggtt
    30961 gtcctggttt tccaaatgaa cactaatggt cttatcagca ctctcttcta ttttgttaat
    31021 tttaatttgc tcataaagtg ctatctgcat cgactcaagt tgacttcgaa taatagccac
    31081 gcaatcggca tcatccttag gaagaatagt aaaggcttct aaaatagtga ctttagaccc
    31141 aagcattgcg aaagcctgag ctaattcaca gcctatagga cctccgccga taacaatcaa
    31201 atgctcaggt tgtactttta ggttaaagat ggtttcatta gttaaataag agaccttgtt
    31261 taagttctgg ataggaggga tggctggaga tgagcctgta gcaatcacaa aatgtttggc
    31321 ccgaataatt ttccttttta atttaaactg ttttggccct ataaaatgcc ctgcttcttg
    31381 aataacctga acacctagag aggtaaaacg ttccaccgaa tcattttttg caatacacgc
    31441 tatcacatta tgcacctgct gcatcacttg caaaaaatca attttcaccg attttgcctg
    31501 aatacctaaa acagtcgaat gtttggtttg ataaaagatt ttggcagtgt tcaatagcgt
    31561 tttagaaggg atacaaccgt aatttaaaca atcacctccc attttgtttg gttcaaccaa
    31621 taccacttgt aatcccaatt gagagcaacc tgcagcgata cttaagcctg cagaaccccc
    31681 gccaataatg gccaaatcac aaaataaatc tttatctttc attttttcag taccctgttt
    31741 accctttttt ttgtaaaaaa caggaatcaa agaaagagcg gctaatccta ataaaggtag
    31801 aataaatttg ggttcaaaaa taatccccag attaggctga aggcttgctg caaatagttc
    31861 gcccaatcca ttaccaacag acacatagac aatagagcca ggaataattc ctataaaagt
    31921 ggctatgata aaagtagaag cgctcacacc caatacggca gaaacgatat taatcaccca
    31981 gaaaggaaat aatgggataa gtcgcaaggt gagcaaataa gaaaatgcat tgtctttaaa
    32041 cccagaacgc atccgcttaa cccaacctga agcacgcttg gaaaacactt cgcctaagga
    32101 cgattggact gcaaaaaaaa gaatcgttgc acctaaagtg gcacttagaa ccaccaaaaa
    32161 agttccccaa agtattccaa ataagaagcc gcctattaaa gtcaaaaaga tagcacctgg
    32221 gatagagagt gctacagcca ctgtataaat tattataaaa acgaaagaga ccagataaaa
    32281 atgtgatttt gttatggcta taagccattc atgattgtct cgtaaactgg caaaacttac
    32341 gtatttattt aagcctaaac ccaaaaaaag acctaataaa actattataa ttaataaggg
    32401 cagccaagtt tttattgcac taattttcat aagatgatgc ccttttcatt attcacataa
    32461 cgttcttact ttcttatcag aagtttttga ctttcgacta aaatgaaaat ccttttttat
    32521 ttgccaatag tggcgttgag ctttaattgg catcaaaagt aaccgtataa gactattatc
    32581 tgatggcttg gaatacccag gtacaccaat tattatagct tgttggggaa tatctaattt
    32641 gattgtttga tgcaacctat cccaaaatga aaatacaacc ccataattac tattggtttc
    32701 attgagataa ttggaatgat gaatgccatg cattcttgga gtaacgataa ttttattaat
    32761 tagccgttca aagcgaaaag gcaatttgat attgctgtgt tgaaaaaaag tatgtgcttg
    32821 aaaaaaaaat tcatagcaaa aaaagagtaa gggactcact ccaatcaacc ctaattgcac
    32881 caacctaaag agactcgaat acataatttc cacacaatga aaacgcatgg cggttgaaac
    32941 atctaagtca ggatcagcgt gatgcacgac atggaatcgc cacaacaacg gaagtttatg
    33001 atttagagca tgccagtagt aaaagcttaa atccattaac aaaaacccaa gtataaacac
    33061 tacccattga ttaaagggaa gcatagatag aaggccgaaa tcatggctaa aggtgaaaag
    33121 gacggtggct tttgctaagg ggctaataaa cagacttgcg atgacataaa ccaagagggt
    33181 aaaggtgaaa ttaattgcta atcgcggcaa taatgggcga gttgcttttc ttaaaggcca
    33241 ctttttctct aaaacaaaca atgacaaata aataaaaata agaatgagta ctaagtaata
    33301 ttgta
//
LOCUS       DAXLDT010000017        32910 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXLDT010000017 DAXLDT010000000
VERSION     DAXLDT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32910
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.017"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..889)
                     /locus_tag="ACF2SP_000659"
     CDS             complement(<1..889)
                     /locus_tag="ACF2SP_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFD2358464.1"
                     /translation="MTVKRSSLGRNLSALLSQSSSSLLANEQPQTEQQLKLAVTCLQP
                     GKYQPRGGMEEAPLNELAQSIKKQGVLQPLLVRELDNGKYEIIAGERRWRASQLAGLT
                     EVPVILKQVDDETAMAMALVENLQREDLNAMDQARAMYRLTNEFALTHQQVADLLCKS
                     RTAVSNFLRLLSLSTPVKKLLENGDIDMGHARALLALDEEQQSQVAQLIIAKNLSVRE
                     TEQLVERVKNGKPELKTHHKDNPMFKEQLNSLAKHLQTKIKLKQGKTGKGTLVIHYEN
                     DHNLQKIIEQLLGNVPEVFL"
     gene            complement(903..1673)
                     /locus_tag="ACF2SP_000660"
     CDS             complement(903..1673)
                     /locus_tag="ACF2SP_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFD2358465.1"
                     /translation="MAKVIAIANQKGGVGKTTTAINLSASLAASKQQVLLIDLDPQGN
                     ATMGSGVDKRQLVHTTNDVLLRDCLAEQACLATTCGYDLIPGNEDLTVAEVSLMERNH
                     RETFLYKALQPIQSNYDFILIDCPPALNTLTINAFVAADSVLIPMQCEYYALEGLAAL
                     LSTIEQVKSTVNSRLHIEGVLRTMYDARNRLCAEVSRQLLEHFPAKVYRTVVPRNVRL
                     AEAPSHGMPALQYDKSSPGAAAYMVLAAEVISKQTVAS"
     gene            complement(1676..2302)
                     /gene="rsmG"
                     /locus_tag="ACF2SP_000661"
     CDS             complement(1676..2302)
                     /gene="rsmG"
                     /locus_tag="ACF2SP_000661"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFD2358466.1"
                     /translation="MIDNTKIRSLLEEGLTAFQLDSIGDLLLDFLLLLNKWNKTYNLT
                     AIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNG
                     KKISFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTEHLVAQ
                     DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE"
     gene            complement(2299..4173)
                     /gene="mnmG"
                     /locus_tag="ACF2SP_000662"
     CDS             complement(2299..4173)
                     /gene="mnmG"
                     /locus_tag="ACF2SP_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFD2358467.1"
                     /translation="MNLEQLYDVIVVGGGHAGTEAALAAARLGVKTLLLTHNIDLLGQ
                     MSCNPAIGGIGKGHLVKEIDALDGAMAKAADQAGIQFRILNASKGPAVRATRAQADRV
                     LYRKAIRTQLQSQANLTIFQQAVDDLKIEGGLVTGVVTQMGLTLKARAVVLTVGTFLG
                     GKIHVGMNQYAGGRAGDPPSIALSKSLRDLDLPVGRLKTGTPPRIDRRTIDFSQMVEQ
                     PGDTPVPVFSYLGTASDHPQQVPCHITHTTEATHDIIRNNLDKSPMYAGVIEGVGPRY
                     CPSIEDKIVRFADKTSHQIFVEPEGLTTEEIYPNGISTSLPFEVQVQFVRTIKGFENA
                     HITRPGYAIEYDYFDPRGLTSFLQTKAIPNLFFAGQINGTTGYEEAAAQGIIAGMNAA
                     LQIKDQELWCPRRDEAYIGVLIDDLITCGTQEPYRMFTSRAEYRLLLREDNADLRLTE
                     KGRQLGLVGDERWESFSKKREAIESTQALLHNSWVRVHHNDLFKEALLNPMQHDCRAA
                     EFLKRPEINYQHLLMMDDLNLPELPQEITEQIEIQNKYAGYIDRQQQEIEKLRKHENT
                     MLPETLDYNDVVGLSSEVIQKLNRIKPTSLAQAGRISGVTPAALSLLLVHLKKSRLPV
                     "
     gene            complement(4304..6217)
                     /locus_tag="ACF2SP_000663"
     CDS             complement(4304..6217)
                     /locus_tag="ACF2SP_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2888 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358468.1"
                     /translation="MATSSLILQSYLKTLKTDEKSELRYEDKVEELLEYNSKAKGGDF
                     LAPLSFYLAILENTDEVMLRYMSEENKQKLIRDLQVAYLLLLTQKKYEEEHQKYENIK
                     TYTQHIKRCEELLDHLNYNQVCKEQKRAPSPEHGYASDGYPVKYLSIFLGKELAEMIV
                     DSMDRKTKTIKESMGWFNEKRLYWVWASSLLKVILAALPDDFFNVGQATEVVKAPDPY
                     TGTLSWALYYFRFSLNMFLLLKHTISGPWMSEREKKTPWTERFLTQWDQRKFTLLNDS
                     IWATGNLICFFWLTGKGALGTWGDVLTLALLVFDISMAIWDYEEQKTRHNKEMLAYEE
                     DIKKLKQLIRKAEEEPGKEDEKLRIIKEYELQLQGLMRAQKESERNWDLQKVSLYAGI
                     AYAVGLMLAFALLAAPFFPVSGPALLAITITGAVLCLAFTVIYNGVKGGMEVYKAHCS
                     AKEAKEGYNNKIELFKLLLEKNPSLDDNEKKFLFLEIKKLKAETEYQKQMVVFQTMHL
                     LRSVILESFIPAVVFASFVFLPLGIGFAVLGATIGVALATNLMINAAFKPAKNEVKAF
                     DEKEYEAFCKDPDNWNKPSKSKQGFFQPKEKKPLLDAVAAKTQEKENDRIDLLDDESS
                     VPLLLSSKNTPNI"
     gene            complement(6445..7881)
                     /locus_tag="ACF2SP_000664"
     CDS             complement(6445..7881)
                     /locus_tag="ACF2SP_000664"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFD2358469.1"
                     /translation="MTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTM
                     KRAMGLPGNQTIIVSNDAHYFICQDQLKDFNAQTTYLLEPCARNTAPAIACAAHYLAN
                     TVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIE
                     AGTTLFSNIKQVLSFREKPDAHSAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANI
                     YQFSQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGC
                     WTAVADANKQDEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVA
                     DKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKL
                     SLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIE
                     VQSGSYLGEDDIKRFDDIYLRKEVELAE"
     gene            7971..8657
                     /gene="queC"
                     /locus_tag="ACF2SP_000665"
     CDS             7971..8657
                     /gene="queC"
                     /locus_tag="ACF2SP_000665"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFD2358470.1"
                     /translation="MKKAVVLLSGGLDSTTCLALAKSQGFACYALSFSYGQRHSAELC
                     AATRIAKHMGAADHKIVTLDTALFGGSALTDASIEVPEFKESPEIPVTYVPARNTIFL
                     AMALGYAESIGARDIFIGASSVDYSHYPDCRPEFIESFQSLANLATKAGIEGDRFTIN
                     APLQYLSKVQAIQLGTELGVDYGLTVSCYQANEAGEACGQCDSCTFRKRGFKSAGVDD
                     PTRYQKCVHI"
     gene            8700..9254
                     /locus_tag="ACF2SP_000666"
     CDS             8700..9254
                     /locus_tag="ACF2SP_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2885 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358471.1"
                     /translation="MEFFMWDKIKEWYKNNKSYLTMLLIGSAVVGLSVTFLALFFPPV
                     LAAFASISIFGMAPLAFLTSLHLPLAVLTLGCIVTGAAFGAILGGLVAWKQSISIAKH
                     VYSYFGHKQEHQVEINEEDTYRYLMGHLEGEASESFEVEVDEEFNDYTSTDSTEDRPN
                     PLNFSTDSNPQALDGGYEMTEFRI"
     gene            9556..10293
                     /locus_tag="ACF2SP_000667"
     CDS             9556..10293
                     /locus_tag="ACF2SP_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2884 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358472.1"
                     /translation="MTLKQFATGVFYLPAAVFSGLTNLFLGSYSKDKRGNYIQDEDGN
                     YVRNRGLIGLTLDAVKYLGTSVANFISNHQKAISVAFWSSLVVAGAAALTVAFWPAAL
                     AAVVNFSVFGVSIASVVGAGYAAQVAATAGVAAALTSTGVYLGATVVNAFNALRECIS
                     NMRSKKPSSNPEFTPVNDETFEEVRRNQFSGLSKGSEQQQTVQLSSSSSEEEEPVHTT
                     KVFAQPKDVTPEVVENPTSTLSLTGSK"
     gene            complement(10349..10918)
                     /locus_tag="ACF2SP_000668"
     CDS             complement(10349..10918)
                     /locus_tag="ACF2SP_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFD2358473.1"
                     /translation="MANNPRLIIGISGSSGIIYGIRLLQVLQKLPIETHLVISKAGQL
                     TRAYETNISAAELKALADVYHPCTDLTASIASGSFKTLGMIIAPCSMKTLGEIAHGIS
                     SNLLTRAADVVLKERRKLVLLPRETPLHLVHLNNMVCITQMGGIICPPVPAFYNHPQN
                     IDDLINDTVGRVLDLFDLDCGLVKRWGET"
     gene            11187..13199
                     /gene="metG"
                     /locus_tag="ACF2SP_000669"
     CDS             11187..13199
                     /gene="metG"
                     /locus_tag="ACF2SP_000669"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFD2358474.1"
                     /translation="MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQ
                     CISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPEN
                     QVLATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
                     CGATYSPTDLINPVSVISGASPVEKESEHYFFDLPLYEELLKDWTRKGHLQAEVTNKL
                     SEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFD
                     EFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKS
                     RGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIAS
                     RCAGFINKRFDNRLSSELNDPKLHNDLLSARESVIDAFVSRDYARAIRQIMDCADKVN
                     QYIDANKPWVLAKDESKLNEVHAICTMGINLFRILITYLKPVLPMMAKASEEFLNSEP
                     LHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPVKENTPVEDANLI
                     SIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRL
                     TVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPDAGALPGMKVK"
     gene            13199..13813
                     /locus_tag="ACF2SP_000670"
     CDS             13199..13813
                     /locus_tag="ACF2SP_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFD2358475.1"
                     /translation="MASVKEIDALLPQTQCGECGYAGCLPYAEALAQGAAPINKCPPG
                     GVETVNALGKLLHVDASPYLKEAIDNTRPPSVAAIKEDECIGCTKCIKACPVDAIIGS
                     SKLMHAIIAHECTGCGLCVDPCPVDCIEMVSLPAAGYDKDLARQRYNAKQMRQLRDEH
                     EKQQIYRERKKLSAHAQDHTQDVQAKQNYILEALARVKAKKTYE"
     gene            13806..14441
                     /gene="nth"
                     /locus_tag="ACF2SP_000671"
     CDS             13806..14441
                     /gene="nth"
                     /locus_tag="ACF2SP_000671"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFD2358476.1"
                     /translation="MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATD
                     VSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCAILIDQHEG
                     QVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNAPLAVE
                     QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL"
     gene            14682..16433
                     /locus_tag="ACF2SP_000672"
     CDS             14682..16433
                     /locus_tag="ACF2SP_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2879 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358477.1"
                     /translation="MLQESIMKWFSSVVNGVKTIGSSLWEGSKWVWNNASLSKITTGV
                     FSYTANTTFQVLEQAIALRDAVPTLVNNPQAKKIAYGMGYILVNDVLPLVALNFANNT
                     AQNYFGEGYDEDASWYAPYSLFLSGLALTNYLVKAYTWRQGVQTVAHVAVLDSLGPSS
                     FNSNKKSLPPSICDELDCNYKRKIKGWGREPLVLMGNDALTYGVSLIPYVGEPLSQVL
                     RVYFNGRYIIRLTTPELCERHKYLAMKPESVLGFGLTFEATSMLMDKALESTVGMPPF
                     LYYRALRHLLLLMHINVAAHMELPKSDLKDKASSFDPFNVYERVNRFVADVIFSGLIK
                     RIPIDFKPDKDAEPLIPLSPALKFGTMVLNMDLEKEKKTTPGLFSKAIDKAYVLVLPP
                     VFRGANGLINDPIVFMYWPTIRSGAISAVEFVEGVGKTKTMATLAWAPKTVAAAINLK
                     FGIPKKVTRFVLMLSKEEDFWNLATAFKLWFERHNIKGEPKLITGSNLALNGVTPLQP
                     TPVSVEKEPIVSPHLLITEKDTKEVVPAKSLIPVRSQPQTGVVVTISPDSLFSTRRRR
                     VNPDQEKVAITELSMTK"
     gene            complement(16546..17610)
                     /gene="corA"
                     /locus_tag="ACF2SP_000673"
     CDS             complement(16546..17610)
                     /gene="corA"
                     /locus_tag="ACF2SP_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFD2358478.1"
                     /translation="MNTHKTKASAKAGLLPGTAVYIGEHPPQPTQVTIHIYDKKNYKR
                     LDSFDTRQVKEAYEAGHNIWIDISGLADIEKITKICSEYNIHPLVIEDLLNTRQRPKL
                     DIVDDYIFVVFKLLQSPPDHLSYNTEQFCLIIKQKLLLTFRESEKYNLSSIYQRLNGE
                     HSLTREYGSDYLTYLIMDYIVDDYFNFVETSAQLLETLEDQLINKPETIKLQALYTIK
                     RRTITLRKIIPPLRDIVHLLLTDDGGLINKKYNLYFRDLYDHCIRLVESIDLHREMSA
                     SMLDIYLSTINNRMNESMKILTLFASIFIPLSFIAGVYGMNFKFMPELDWRYGYPMIL
                     VTMVLLAILMLYFFKRKKLF"
     gene            17880..19169
                     /locus_tag="ACF2SP_000674"
     CDS             17880..19169
                     /locus_tag="ACF2SP_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HFD2358479.1"
                     /translation="MNQSLSPQQSPGFLFIAVSMITCLILLINVSFKIILLGGLVFAI
                     NSLICPLIAALFLFALRNCTFKEQRHLLNICLMTLYMFCIGVYVLVNLPAAEYMHDNP
                     AYQVIFEEIPKKFFATTIAFALSFYLPHLLFCTKNDRVLSTPKQCVLLALLGGVSFFC
                     LDFYLLFSAPHAHSFKQIFIDSFMISSLLLLIIGVVYLTFLLNDKQHKSKALIETTEL
                     TLFPIYQYLICFAVTVMLVCLACEYRIIAINKDLVLSASCIFFPITIIISSIVGELWG
                     YQANLKLALALLAAQFIFDMLLMGSVALPSPPFFNLNPFYHYIMPRRLSAASLSLFVT
                     FLGNAMLLHYLKYSKWKINRSLRILIANISVNSLLCLVDYSLLYGGIYPYEQIINLAV
                     SVWQYKLLTALIALPVILWMCKILERNYSLVVQTERN"
     gene            complement(19196..20437)
                     /locus_tag="ACF2SP_000675"
     CDS             complement(19196..20437)
                     /locus_tag="ACF2SP_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFD2358480.1"
                     /translation="MPSAVIILLIILIAIALRQVVRVIIPIWAIMATGAIAVLLCQQI
                     TPSRAFTAVEPDVMLYLFGVFFISQAAEESGYLAFLTDQIFYYANNGKQTLMLIVFIL
                     GLSSAVLMNDTIAIIGTPIILQLCQSHKNLIKPLLLALAFSITIGSTMSPIGNPQNLL
                     IAIKGEMASPFFEFFKHLAVPTFLNLGIVYFFILFLYRNELKEPIKKPVLPLINDRSN
                     LTLVKMSLLMMLVLTLIKILTDFLHYSEFQLNFSYIALLSSVPIWFSKHRWNYIKRLD
                     WGTLVFFASTFILMQSVWDSGFFQTGMSDSHIDVSHIVVIFIMSIALSQLISNVPLVT
                     IYLPLLMSHNHSSITLLSLAAGSTIAGNLSILGAASNIIIIQNSEKRGVRGFGFLEFI
                     LVGAPLTLLNLLVYISFLQKI"
     gene            complement(20454..21839)
                     /gene="glmU"
                     /locus_tag="ACF2SP_000676"
     CDS             complement(20454..21839)
                     /gene="glmU"
                     /locus_tag="ACF2SP_000676"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFD2358481.1"
                     /translation="MNLQIIILAAGQGKRMYSDTPKVLHHLAGKPLLTHVVETAQQLN
                     PDAIHVIYGHGGEQIKSSLPNLPVHWVHQAEQLGTGHAVLQAMPHIPDDAYVLVLSAD
                     VPLIQVGTLQSLIECSQRQNPDHSVLALLVAELENPSGLGRIIRNNQGEIYSIVEEKD
                     ANEQVKNIKEIYSGVCCTLANNLKKWLPQLSNSNAQGEYYLTEIISLAVQNKTPITSL
                     TAKNSFEVQGINNRQQLQQLERTWQQRAANQLMEKGATLADANRFDLRGELYCGKDVY
                     IDINCIFTGKVVLGNGCKIGPNCSLTNVTLGDGCEVYANSVLEGCHIANDCHIGPFAR
                     LRSGTQLASHCKIGNFVETKKAIFDEGTKASHLSYLGDVLLGKNVNVGAGTITCNYDG
                     VNKHQTIIEDGVFIGSDTQLVAPVTVGANATIGAGSTIRRNVPPDELTLTESRQKTIY
                     GWKRPVKRERD"
     gene            21961..22845
                     /locus_tag="ACF2SP_000677"
     CDS             21961..22845
                     /locus_tag="ACF2SP_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2874 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358482.1"
                     /translation="MNIFLKVLKKLMKEKLSELSANLKWKSETIGYNTKLSIDQKNVI
                     DKLEESIVLFKDSGNDEADLLSINALIDTARIQIQSIRESYGEPRDKGESVTCLNNLK
                     NHSNDFLGKLKKFDFDLLNKAYTETPENLVYYHAAIYLGDEIFSPRTGIDYEIRTKKE
                     VQLAVRLQALSERINPSHNLEEQRKRALQVLQDLAQDNQNAIKKDKGFSLPGLSFWGV
                     SVVTPSDWFSSGEGRFGVEFNTAVRTIQGMTESQFERPTVKQTTAKQDETKPSDVKKT
                     EATDEEDEEHSSENTHTM"
     gene            complement(22943..23953)
                     /gene="ansA"
                     /locus_tag="ACF2SP_000678"
     CDS             complement(22943..23953)
                     /gene="ansA"
                     /locus_tag="ACF2SP_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFD2358483.1"
                     /translation="MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQD
                     MPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSF
                     MLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
                     QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPRPKAPFHLQTITPHFIANFRLFP
                     GFASDVLEYILNQPLRGLILETYGAGNAQNNDPRFLNLLKEACGRGVIIINCTQCHQG
                     KVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIPEIKKLIETNLCGEL
                     QI"
     gene            24306..24833
                     /locus_tag="ACF2SP_000679"
     CDS             24306..24833
                     /locus_tag="ACF2SP_000679"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFD2358484.1"
                     /translation="MVIDRAGYRLNVGIILVNDSDRVFWGRRSGHDAWQFPQGGLAPG
                     ETAMQAMYRELHEEVGLDKGDVEILGSTRRWLKYRLPKQYLRHGSEPLVIGQKQKWYL
                     LKLVTSEQKVRLDLSDSPEFDSWRWVDFHEPEQQVIFFKRQVYIQALKELEPLLKKER
                     RTPYGLKRKRGNQRA"
     gene            24834..27128
                     /gene="ptsP"
                     /locus_tag="ACF2SP_000680"
     CDS             24834..27128
                     /gene="ptsP"
                     /locus_tag="ACF2SP_000680"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFD2358485.1"
                     /translation="MLKILKRIVQDVTAASDLKEALGILVQRINTAINAEAVSVYLID
                     NKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLI
                     GEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEAT
                     GELAELTQPKKGIGVDKVEAAYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEE
                     LEDEINAFYEALETTRDDIRRLSKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVI
                     REEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRVLAELQRTQREEIVY
                     PKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKV
                     ELVSRRALIVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYR
                     VSLQVNTGLAMDAGLSMSVGAEGVGLYRSEVPFMSRDRFPSEDEQTIIYRQTLKAFAP
                     RLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMRASEELD
                     NLRIMLPMVTTLSEVEEAVYLIEQAFEELVEEGCEIVKPKIGVMIEVPAAAYLAREMA
                     KRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSI
                     CGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRNFAIANARKILAEVLEFEHPA
                     EIRFHLQKALEEEGLGGLIRAGKS"
     gene            27125..27931
                     /locus_tag="ACF2SP_000681"
     CDS             27125..27931
                     /locus_tag="ACF2SP_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFD2358486.1"
                     /translation="MISGELLINGVIYIIIGAFAGIMAGALGVGGGLIVVPGLVYIFQ
                     HSQVIPEDILMHVAAGCSLAVMIFTSYASLKAHNKMDEILLPLFKKLCPGLVLGTILG
                     SIMASFIPTHLLKIIFGLFLLGVAMKMLLDIRVSHTDKFPGYWLNTLVSSSIGSISGL
                     LGVGGGVIIIPYLTYCGVPVRKIAAVSNLGTFAVGLVGTIMFMITGTLDMTKISYSTG
                     YIYWPAVLGVAIPSSLIAPFGAKLNYLLPVNYLRYGFIVILITTAIHMFL"
     gene            27949..28719
                     /gene="lgt"
                     /locus_tag="ACF2SP_000682"
     CDS             27949..28719
                     /gene="lgt"
                     /locus_tag="ACF2SP_000682"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFD2358487.1"
                     /translation="MLTYPNINPIAFSLGPLKVHWYGLMYLIGFIGAWLLGYWRIKHY
                     KLNWNNDQLSDLIFYSALGVILGGRVGYMLFYDIQEFIHHPWVLFKIWEGGMSFHGGL
                     LGVVIAAWLFCRKYGKTFLEVGDFVAPLVPLGLAAGRLGNFINGELWGRVTDVPWGMI
                     YPHVDDQPRHPSQLYEFGLEGVALFILIWCYASKPRQQGRVCALFLMGYAICRLIAES
                     FRQPDSQLGFVAFGWLTMGQVLSIPMLLIGIWLWWAKR"
     gene            28716..29510
                     /gene="thyA"
                     /locus_tag="ACF2SP_000683"
     CDS             28716..29510
                     /gene="thyA"
                     /locus_tag="ACF2SP_000683"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFD2358488.1"
                     /translation="MKTYLQLLEHILQQGVEKSDRTGTGTLSVFGYQMRFDLTKGFPL
                     VTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWP
                     TADGHTIDQLSDVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
                     CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQI
                     QLTREPLPLPSLTIKRKPASLFDYAYEDFEFLNYQSHPAIKAPIAV"
     gene            29586..29855
                     /locus_tag="ACF2SP_000684"
     CDS             29586..29855
                     /locus_tag="ACF2SP_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358489.1"
                     /translation="MKKIVVFVLLAVVCSAGFPSKLSKFLNKMDAEQKQRDAQEWQQD
                     MNFGDYAFRLKERYIDDRGQRCRDYEFRGRSNPYRHGYFTVCDDR"
     gene            complement(29973..30365)
                     /locus_tag="ACF2SP_000685"
     CDS             complement(29973..30365)
                     /locus_tag="ACF2SP_000685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFD2358490.1"
                     /translation="MTEINKIIHKKTFDIAWGDMDALGHVNNARYFDYFQEARIDWLR
                     ELDIKMTGQTGPVVIHVACTFLKPIVYPATVTIHSKVHSLGNSSMIMDHDLYQEETLM
                     AQGVSKIVWIDYTQNKSVPLPDIIRNLV"
     gene            complement(30362..32539)
                     /locus_tag="ACF2SP_000686"
     CDS             complement(30362..32539)
                     /locus_tag="ACF2SP_000686"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFD2358491.1"
                     /translation="MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGI
                     VINKVCGQQTGHSLKKITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKY
                     RLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
                     NGNQIATLLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVASILEHIHHYQCFL
                     LQGVTGSGKTEVYLHVIAKILEQGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSN
                     LNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSA
                     RDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLR
                     SQMVQHGLALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLT
                     LHKQAGQMICHHCGLTQRIPLFCNNCQSKELIPVGSGTQRIYEFLSHQFPDTNVLRID
                     RDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGFYNQDFR
                     AIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQE
                     AEMPPFQFLAVIRAQGKTVNKVDQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQ
                     LLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVDPMDLS"
     gene            complement(32540..>32910)
                     /locus_tag="ACF2SP_000687"
     CDS             complement(32540..>32910)
                     /locus_tag="ACF2SP_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HFD2358492.1"
                     /translation="ALTTWVEENGMTFDYRYYKERIHKLCRYLNQTFLMPQFSPFLEY
                     SEDEEYYYFIFNKKKIPFLKEDVTLMPLTNITVEELSRWFVNELIKEKEELDKHRIEK
                     VVVKVFSAPGQSASHEWLRS"
BASE COUNT         9343 a         6267 c         6991 g        10309 t
ORIGIN      
        1 ctaaaaaaac ttcagggacg ttacctaata attgctcaat aattttttgc aaattatgat
       61 cgttttcata atgaataacc aatgttcctt taccggtttt cccttgtttt aatttgattt
      121 tagtctgtaa atgttttgct aaagagttta actgctcttt aaacatagga ttgtctttat
      181 gatgtgtttt taattccggt tttccgtttt ttactcgctc aaccagttgc tccgtttctc
      241 tgaccgataa atttttagca atgatcaatt gagcgacttg actttgctgc tcttcatcca
      301 aagcaagtaa cgctctggca tgacccatgt ctatgtcccc attttccaaa agcttcttta
      361 ctggcgttga aagagaaagc aaacgaagaa aattgcttac cgcggttctg gatttgcata
      421 agagatcggc tacttgctga tgggtaagag caaattcatt ggtgagtcta tacatggccc
      481 ttgcttgatc catggcattt aaatcttcac gttgcaagtt ttcaaccagc gccattgcca
      541 tggccgtttc atcatctact tgtttaagga taacaggaac ttctgtaaga cctgccagtt
      601 ggctggcacg ccagcgtctt tctcctgcga tgatttcata ctttccatta tctaactcac
      661 gaaccagtaa gggctgtaat accccttgct ttttaatgga ttgcgcaagt tcattgagag
      721 gggcttcttc cattcctcca cgaggctgat acttgcctgg ctgcaaacat gtcactgcga
      781 gtttgagttg ctgttctgtc tggggttgct cattcgctaa taaagagctg cttgattgac
      841 ttaataaagc agataaatta cggcctaaac tactgcgttt tactgtcatt acttacccca
      901 aattaactgg ctacagtttg tttacttatc acttcggcag cgagaaccat ataagctgct
      961 gctcccggtg aggatttatc atattgcaag gcaggcattc catgacttgg cgcttcggcc
     1021 agtcttacat tgcgtggcac aacagttcta tacacttttg cagggaaatg ctctaataac
     1081 tgcctggata cttcagcgca taatctgttg cgcgcatcat acatggtacg aagcacgccc
     1141 tcgatgtgca aacgcgagtt gactgttgat ttcacttgtt caatggttga taacaaagca
     1201 gccaatcctt ccaaagcata gtattcacac tgcatgggta tcaaaacgga gtcagcagca
     1261 acaaatgcgt taatggttaa tgtattcaaa gcaggaggac agtcaattaa aataaaatca
     1321 taattagact ggatgggttg caacgcttta tataagaaag tttcccgatg attgcgctcc
     1381 atcaggctaa cttctgcaac agttaaatct tcgttaccgg gaattaaatc atatccgcaa
     1441 gttgtagcaa ggcatgcctg ctcagccaga cagtcgcgta acaagacatc gttggttgtg
     1501 tgaaccaact gtcttttatc taccccacta cccatcgttg cattaccctg aggatctaaa
     1561 tcgatcaaca aaacttgctg tttggatgcg gcaagagatg cagataaatt gatggctgtg
     1621 gttgttttac ctactccacc tttttggttg gcaatggcta tgactttcgc cataattatt
     1681 ccttgttcgt gttgttaata agcacgcagc aacgctctcc ttcgataccc gggacagcat
     1741 atctctcaac tcgatatgtt tgatgaatcg ggaccaattc agtatccgga aaccgccctt
     1801 tcatggctaa ccacaaacca tcctgagcga caagatgttc agtccatttt atcatttgtt
     1861 cgagactgct aaatgctctg cttattactg tatcaaaacc ttggtttggg tggtagttct
     1921 ctactcgaat ttgtattggt tcaatgttct ttatattgag ttgacgtttt acttcattga
     1981 gaaagctaat ttttttccca ttactgtcca ataatacaaa ttgtaagtca ggtttggcaa
     2041 tagccaaagg gatgccaggt aaacccgggc cagtacccac atcgataata tggtttccct
     2101 ttatccaggg taaaatagcc agactgtcga ataggtgctt gctgaccatg gtttcaatat
     2161 cacgtattgc ggtgaggtta tatgttttat tccatttatt gagcaggagc aaaaaatcaa
     2221 gcaagaggtc gccgatggaa tccagttgaa acgcagtcag accctcttca agcaatgatc
     2281 ttattttcgt attatctatc atactggaag ccgactcttt ttcagatgaa caagtaaaag
     2341 agacagagcg gcaggtgtta cgccagaaat ccgcccggct tgagccaaag aagttggttt
     2401 aatccggttt aatttttgga tgacttcact tgataaacca acgacgtcat tataatccaa
     2461 agtctcaggg agcatggtat tttcatgctt gcgtaatttc tcaatttctt gttgttgcct
     2521 gtctatgtac ccggcatatt tattctgaat ttcaatttgc tctgttattt cttgtggcaa
     2581 ttcgggtaaa ttcaaatcat ccatcattaa aagatgctgg tagttaattt ctggccgttt
     2641 caaaaattca gcggcgcggc aatcgtgttg cattgggtta agcaaggctt ctttaaacag
     2701 atcattgtga tggactctca cccagctgtt gtgtaacaaa gcctgagttg attcaatcgc
     2761 ttcacgtttt ttggaaaagc tttcccaccg ttcatcacct accaggccaa gctgccggcc
     2821 tttttcggtc agtcttaaat cggcattatc ttcacgcaat aacaaacgat actcggctct
     2881 tgaagtaaac atcctatagg gttcttgagt tccgcaagta atcaaatcat cgataagtac
     2941 gcctatgtat gcctcatctc tgcgaggaca ccacaactct tgatctttga tttgcaaggc
     3001 agcattcatg cccgctataa ttccctgcgc tgccgcttcc tcatatccgg ttgtcccatt
     3061 gatttgcccg gcaaaaaaca aattaggaat tgccttggtt tgcaaaaatg aggttaatcc
     3121 acgtggatca aaataatcat actcgatggc gtaacctggc ctggtgatgt gggcattttc
     3181 aaaaccttta atggtgcgca caaattgcac ttgtacctcg aaggggaggc tggtagaaat
     3241 tccattaggg tatatttctt ccgttgtcag accttccggt tccacaaaaa tctggtgaga
     3301 tgttttatca gcaaagcgaa caattttgtc ttctatcgag gggcaataac gaggtccaac
     3361 tccttcaatc actcccgcat acattggcga tttatccaaa ttatttctta ttatgtcatg
     3421 agtggcttct gtagtatggg taatatggca tggcacttgt tgaggatggt cgcttgccgt
     3481 tcccaaatag gaaaatactg gaaccggggt atcacctggt tgttccacca tctggctaaa
     3541 atcaatggtg cgtctgtcaa ttcgaggagg agttcctgtt ttcaaacgtc ctaccggtaa
     3601 atccaaatcc cgcaagcttt tagataacgc aatggatgga ggatctcctg ctctgcctcc
     3661 tgcgtattgg ttcataccga catgaatctt cccacccaga aaagtcccta ctgttaatac
     3721 aacagcacgc gccttgagcg tgagccccat ttgggttaca acaccagtga ctaatccgcc
     3781 ttcaattttc aaatcatcaa cagcttgttg aaagatggtt aaattagctt gtgattgaag
     3841 ttgtgttcga atggcttttc tatacaaaac acgatccgct tgtgcgcgtg tggctcgaac
     3901 cgcaggtcct tttgaggcat taagaattcg aaattggatc cccgcctgat cggcggcttt
     3961 agccatcgct ccatcaaggg catcaatttc tttaaccagg tgacctttac caatgccccc
     4021 tatggctgga ttgcaggaca tttgtccaag caagtctatg ttatgggtta ataacaaggt
     4081 ttttacaccc aggcgtgctg ctgcaagcgc tgcttctgta ccagcatgcc ctccgccaac
     4141 gactatgaca tcatataatt gttcgagatt catgtttaac cattagttat gccaaaatta
     4201 taaattggat ttttggatta tctgcaaaaa tgatccaagc caacatataa aaaaatgagc
     4261 aattatacac ttttttggca attgtcccaa gatgtaataa agattatata ttgggcgtgt
     4321 ttttgctact taataacagg ggtacactgc tctcatcatc cagcagatcg attctgtcgt
     4381 tttctttttc ctgtgttttt gcagcgacgg catctaaaag aggttttttc tctttaggct
     4441 gaaagaaccc ttgttttgat ttggagggct tattccaatt atccgggtct ttacaaaaag
     4501 cctcatattc tttttcatca aaggctttga cttcattttt cgcaggtttg aaagcagcgt
     4561 tgatcattaa gttggtcgcc aaggcaacac caattgtggc gcccaaaaca gcaaatccaa
     4621 ttcctaacgg taaaaacacg aagctggcaa aaacaacggc tggtataaac gattccaaaa
     4681 tcaccgaccg tagcaaatgc atggtttgaa aaacaaccat ttgcttctgg tattcagttt
     4741 ctgcctttaa ttttttgatt tccaggaaga gaaatttttt ttcattatca tccagtgatg
     4801 gatttttttc caataagagt ttaaagagct caatcttgtt attatatcct tctttcgctt
     4861 ccttggcgga gcaatgtgct ttgtagactt ccataccgcc tttcacgcca ttgtaaatca
     4921 ctgtaaaagc caggcaaaga acagcgcctg ttatggtgat tgccagtaaa gccggtccgg
     4981 aaacggggaa gaaaggcgca gccagtaatg caaaagcgag catgagtccg acagcataag
     5041 ctatgcctgc atataaactg actttttgca ggtcccaatt tctttcagat tctttttgcg
     5101 ctcgcattaa accttgcaat tgcaattcgt attctttgat tattctgagc ttttcatcct
     5161 cttttccggg ttcttcttcc gccttcctaa ttaactgctt taatttttta atgtcttcct
     5221 cataagccag catttctttg ttgtggcgag tcttttgctc ttcgtaatcc caaatggcca
     5281 tgctgatgtc aaagactaac agtgccaaag tcaatacatc accccaagtc ccgagagcgc
     5341 ctttaccagt aagccagaaa aagcaaatca agtttcctgt tgcccagatg gaatcattga
     5401 gcaaggtgaa ttttctttga tcccattggg tgaggaatcg ttctgtccat ggtgtctttt
     5461 tttccctctc actcatccat gggccactaa tcgtgtgctt gagtaacaaa aacatgttga
     5521 gggaaaaacg aaaataatat aaagcccagc tcagggtacc tgtatagggg tcaggggctt
     5581 tgactacctc tgttgcctgg ccgacattga aaaaatcatc aggtaatgca gcgagtatca
     5641 ctttgagcag gctggatgcc caaacccaat acaaacgctt ttcattaaac cagcccatcg
     5701 attctttgat ggttttggtt ttacgatcca tagagtcaac gatcatctct gccagctctt
     5761 tacccagaaa aatgctcaag tacttgactg gataaccatc actggcgtaa ccatgttctg
     5821 gagagggggc tcttttttgt tctttgcaga cttgattata attgaggtgg tctagcagtt
     5881 cttcacatcg tttaatgtgc tgcgtatatg tttttatgtt ttcgtatttc tgatgttctt
     5941 cctcgtattt tttttgtgtg agtaaaagca agtaggcaac ttgcaagtct cgtatcagtt
     6001 tctgcttgtt ttcttcgctc atgtaacgaa gcatcacttc atcagtgttt tctaaaatag
     6061 ccagataaaa actcagtgga gcaagaaaat cgcctccttt ggctttgcta ttgtattcaa
     6121 gaagttcttc tactttgtct tcatagcgta gttcagattt ctcatcagtt ttgagagttt
     6181 tcaggtaaga ttgaagaatt aaactactgg ttgccataaa gtcatctctt ttttaatttt
     6241 tgttaattac ctatataaat gatactataa aaagcaaatt tgttcttttt tataacacag
     6301 tatttatttt aggtacaaga tgaatgcgat tggctttaca gtattgatac taaaatttgc
     6361 aggaataggg atgaagtgta ttgcaactta ggcaagaggg ctaagcacag tgagtggaat
     6421 caagcgcgtt attttcgata gacatcattc tgccagttca acttcctttc ttaaataaat
     6481 atcatcaaag cgtttgatgt cgtcttcgcc cagataggag ccactttgta cttcgataac
     6541 ataaagtggt tcattatgcg ggttacttaa tcgatgcaat gcgtttttcg gaatgtaggt
     6601 ggattggttt tttgataatc ggaacgtatt gtttccatta actacctcgg cctcaccgcc
     6661 aacgataacc caatgctcag cccgatgttg atgcgtttgt aaagagagtt ttgcaccagg
     6721 cttcaccata agccgtttta ctttaaatga ggtgccctct gccagcactt cataataccc
     6781 ccatggtctg ggaacacgtt gatgatcttg agttaaatga ggatgatctt tgcgtaagga
     6841 gtgaaccaaa tctttcactt gttgcgaata acgtttatcg gcaactaaca cagcgtcact
     6901 ggttgcaact atgatttgat cctgaattcc aacggtagtg actaacaact cttcactatt
     6961 aatgaggcaa ttgtgactat cctgggcgat gacatttcca atgagagtat tgccttgttc
     7021 gtcttgttta ttcgcgtcgg ccacagcggt ccagcagccc agatcactcc attgtattga
     7081 aatgggtatt acaacagcct tgtctgtttt ttccattatg gcatagtcta tagactcttc
     7141 tttacattga gaaaaacaat ccagatcaag gcgcaaaaaa tcatgatgat gttgcgcaag
     7201 ggtcaatgcc tgttgactga attggtagat gttggcttca aattgttcta gctcttcaag
     7261 ataaacccct gcacgacaaa tgaacatgcc gctattccag aaataattcc ctttactgac
     7321 gaattgtgca gcagagtgcg cgtctggttt ttctctaaag ctcaaaactt gctttatatt
     7381 actgaataaa gtggtgccgg cctcaatgta gccataaccg gttttggggc tatcaggctt
     7441 tatcccaaat gtcactatcg cattgttttc tgaggcaaat tgtcctccct ctaacatggc
     7501 ggccagccag atttcatcat cggctatcca gtgatccgag ggtaaaacca gcataacagc
     7561 atcctttcct actgtgtttg ccagataatg ggctgcgcat gctatcgcag gggctgtgtt
     7621 tcttgcacaa ggctcaagca ggtaagtggt ttgtgcatta aaatctttta attgatcctg
     7681 acagataaag taatgagcat cattacttac gataatggtt tgattgccag gcaatcccat
     7741 cgctcttttc atggtttgtt gaattaaaga caattcgcca tttaatttta aaaattgctt
     7801 aggaaaattt tgtcttgata aaggccatag tcgagtgcca gagcccccgg ctaaaattat
     7861 tggatataga ggtgtcgtca tcacattcca tattgtggca taattgacct gcattttagc
     7921 aggaattttt aagagcaagc aattgttcca attttttgag gttttgtatt atgaaaaaag
     7981 ctgtggtctt gctttctggc ggtttggatt caacgacttg tctcgcgttg gctaagtctc
     8041 aggggtttgc ttgttatgca ttaagctttt cttacggcca gaggcattcc gcagaactgt
     8101 gtgccgcaac tcgaatagcg aaacatatgg gtgcagctga tcataaaata gtcaccctgg
     8161 ataccgcttt atttggtggt tccgctttga ctgatgcatc tattgaggta cccgaattca
     8221 aggaaagccc ggaaatacca gtcacctatg tcccagcccg taatactatt tttttggcta
     8281 tggctttggg ttatgctgaa tcaattggtg ccagagatat ttttattggt gccagttctg
     8341 ttgattattc tcattatcct gattgtcgcc cggagtttat tgaatctttc caatctttgg
     8401 caaacttagc aacgaaagca ggaattgaag gcgatcgctt tactatcaat gctcctctgc
     8461 aatatctgag caaagtgcag gctattcaat taggtactga gcttggtgtt gattacggat
     8521 taacggtttc ctgctatcaa gcgaatgaag caggtgaagc ttgcggacag tgtgatagct
     8581 gtacttttag aaagcggggt tttaagagtg ctggagtaga tgatcccact cgatatcaaa
     8641 aatgtgttca tatttaaccc atacagttaa attaatgata taatccctga aattttggtt
     8701 tggagttttt tatgtgggat aaaatcaaag aatggtataa aaataacaag agctatttga
     8761 caatgctttt gattggatcg gcggttgtag gtttgagtgt tacattcctg gctctgttct
     8821 tccccccggt tctggcggca tttgccagca taagtatttt tggaatggca ccattggctt
     8881 ttttaacgtc attgcatctt cctctcgctg tgctaaccct aggctgtatt gttactggag
     8941 ctgcttttgg agcaatttta ggaggcctgg tggcatggaa acaatcaatt agcattgcta
     9001 aacatgttta ttcgtacttt ggtcataagc aggaacatca agtcgagatc aatgaagagg
     9061 atacctaccg ttatttaatg ggtcatttgg agggagaggc cagtgagtct tttgaagtcg
     9121 aggtggatga agaatttaat gattatactt ctactgattc tactgaagac agaccaaacc
     9181 cattgaattt ttcaacagat tcaaaccctc aggctcttga tgggggctat gaaatgacag
     9241 aatttagaat ctaaaattcg agcacaaatc aaatatatct tcagggttta cgaaaaacgt
     9301 tgatcgcttg aatttgatcg tttggcttat tcaagcatct taattcatgg cattttttgc
     9361 ttctatcttg ggttttcgaa aatcctgatc acacattgta aattttatcg agctttcggc
     9421 aaaaacctgg ttgagatgaa gacaacaaaa tgaacttact ttacactttt gtaacaacat
     9481 attgcgcaca attaattcac atttaggaaa tgggttgcta caatctgctc cgatttataa
     9541 ccttggagta aaaaaatgac attaaaacaa tttgcaactg gcgtatttta tttgccagca
     9601 gccgtttttt ctggtttgac taacctattt ttaggttctt actcaaaaga caaacgcggt
     9661 aactacattc aagatgaaga tggtaactat gtaagaaacc gtggtttgat tggtttgact
     9721 ctggacgctg ttaaatattt aggaacaagt gttgccaatt tcatttctaa tcatcaaaaa
     9781 gcaatttcag ttgcattttg gtcttcttta gttgttgctg gtgctgctgc tttaaccgtt
     9841 gctttctggc ctgccgcctt ggctgctgta gttaacttct ctgtttttgg agtatcaatc
     9901 gcttcagtag ttggtgctgg ttatgcggct caagttgctg ctacagccgg tgttgctgct
     9961 gctttaacca gtactggtgt gtatcttggt gctactgttg ttaatgcttt taacgcatta
    10021 agagagtgca tctctaatat gcgtagcaaa aagccttcca gcaacccaga gtttacacct
    10081 gttaacgatg agacatttga agaagtacgt agaaaccaat tcagtggctt aagcaagggt
    10141 tctgagcaac aacaaactgt acaactttcc agctcaagct ctgaagaaga agagcctgtc
    10201 catactacta aggtatttgc acaacctaag gatgttactc ctgaagtggt tgagaaccca
    10261 acttctactc tatctctcac tggctcaaag taatataaaa atattacaag caagtggtca
    10321 ttaaaaacgc ggctttagcc gcgttttttt atgtttcgcc ccagcgtttc accaatccac
    10381 aatccaaatc aaataaatcg agtactctcc caacggtatc attgattaag tcgtctatgt
    10441 tttgcggatg attataaaaa gcgggcactg gagggcagat aatgcctccc atttgtgtaa
    10501 tgcacaccat attgttcaga tgaacaaggt gcaaaggtgt ttccctcggc aagagtacca
    10561 attttctgcg ttctttaaga acgacatcag cagctcgggt caacaggttg ctactgatac
    10621 catgcgcaat ttcacctaag gttttcattg aacagggcgc aatgatcata cccaaagttt
    10681 taaatgagcc gctggcaata gatgcggtga ggtcagtgca gggatgatag acatctgcta
    10741 aagctttgag ttctgcggca ctaatatttg tttcataagc acgggtcaat tgccctgctt
    10801 tgctaatgac taaatgcgtt tctatgggta gcttttgcaa gacttgtaat agtctaatcc
    10861 catagattat gcctgatgag ccactgatgc ctatgataag tcttgggttg ttggccatgt
    10921 gtattcactc gttgaaaatt taatttggtc aggacttacg acctttatta aaaacatttt
    10981 ttaccttgac cttgtggttt ctcgtagtcc tgttgatatt aacaaattat aagagaataa
    11041 gtagtactta aattatcgga tcatctcggt aaaagttaac acttcataaa taactaccaa
    11101 taaaaacctg ttttctattg cagcatttcc attgtctttg cgttataatt agtcgcttta
    11161 ttataggtga tgagtattct ataactatga ctagtgaacg aaaaatgtta gtgaccagtg
    11221 ccctgcctta tgccaatggg catttgcatc tgggacattt ggttgagcat attcaaacag
    11281 atatttgggt ccgtactcac aaaatgttgg gtattcaatg tattagcgtt tgtggggatg
    11341 acgctcatgg aacacccatt atgctgaaag cagagcagct gggaattact ccggaagctt
    11401 taaccgccga aattaagcta agtcatgaaa aggattttaa agccttcgcc attgattatg
    11461 attattacca taccactcat tcacctgaaa atcaggtatt ggcgactacc atatttgaac
    11521 gtttgcaggc aggcggtgat atagtcaaga aaacaattcg ccagttttat gatcccgtaa
    11581 agcaaatgtt tttgccggac aggtatgtga agggaacttg ccccaaatgt gctgctgtcg
    11641 atcagtatgg cgataattgc gaggtttgtg gcgccactta ttcgccgacg gatttgatta
    11701 accctgtttc tgttatatcg ggagccagcc ctgtagaaaa ggaatcagag cattattttt
    11761 ttgatttgcc gctttacgaa gagttactta aggattggac aagaaagggg catttacaag
    11821 ccgaagtaac gaataaactc agcgaatggt ttgaggcggg tttaaagcaa tgggatattt
    11881 ctcgcgatgc tccctatttt ggtttcccta ttcccggtgt tccggataaa tacttttacg
    11941 tttggttaga tgctcccata ggctatatgg caagctttaa aaagtattgc gatgagcgtg
    12001 gcgtttcttt tgatgagttt tgggataagg cttcaaaaac agaattatat cattttgtag
    12061 gcaaggatat tgtgtatttt catgctttgt tctggccagc tatgttagct gccagcggtt
    12121 tcagaacacc tactgccgtg tatacccatg ggtttttaac ggttgagggg cagaaaatgt
    12181 ccaaatcgcg cggtactttt atagaagcac gcgcttatct ggctcacttg catcctgaat
    12241 atctgcgcta ttatttcgct gccaaattga atggtcgagt agacgatttg gatttgaatt
    12301 ttgacgattt tgtcaatagg gtgaatgcgg atctggttgg gaaggtagtg aatattgcaa
    12361 gccgatgtgc gggatttatt aataagcgct ttgataatcg gttgagctca gagttgaatg
    12421 atccaaaatt acacaacgat ttgctgagcg caagagagtc cgtgattgac gcatttgttt
    12481 caagagacta tgcgcgagca ataagacaaa tcatggattg tgctgacaaa gtcaatcaat
    12541 acatcgatgc caataaacct tgggtattgg ctaaagatga aagtaaacta aatgaagttc
    12601 atgccatatg cacaatggga atcaatttat tccgcatttt aataacctat ttaaaacctg
    12661 tcttgccaat gatggccaaa gcttcagaag aatttttaaa ttctgagcct ttacactggg
    12721 gttctatcga taagcctttg ctaaaccatc gtatcaatac ttttaagcct ttgatggtta
    12781 gagtagaaaa agaaaaaatt gaagcgatgt tggttcaaag caaggaatct ctgatgacta
    12841 ccccagtaaa agaaaatacg ccagttgagg atgctaacct aatcagtatt gaggactttg
    12901 ccaaagtcga tttacggatt gcaaaaattg tcaatgcaga gcctgtggaa ggcgcagata
    12961 aactaatgcg cttgatttta gacttgggtg atgctcaaaa gcaggttttt gccggcataa
    13021 aaaaggctta tgatgctgaa gaattgattg ggcgtttgac agtaatggtt gctaatttgg
    13081 agccccgcac tatgcgtttc ggtgtttctg aaggcatggt attggcggca ggggatggac
    13141 agggaattta tttattacaa cctgatgcag gagcacttcc tggcatgaag gtgaagtaat
    13201 ggcctctgtc aaagaaattg atgcgttatt gccgcaaacc cagtgcgggg aatgcggata
    13261 tgcaggttgt ttgccttatg ctgaagcgct tgcccaagga gcagccccta ttaataaatg
    13321 ccctcctggt ggtgttgaaa ctgtaaatgc tttgggaaaa ttactgcatg ttgatgcatc
    13381 tccatatttg aaagaagcaa tagataatac tcggcctccc tcagttgctg ctatcaaaga
    13441 ggatgaatgc attggttgta ctaaatgcat caaggcctgc cctgttgatg caattattgg
    13501 tagtagtaaa ttaatgcatg ccattattgc tcatgagtgt actgggtgtg gcttgtgtgt
    13561 ggatccttgt cctgttgatt gcatcgagat ggttagtttg cctgctgctg gatatgataa
    13621 ggatttggcc cgccagcgtt ataatgccaa acaaatgcgc cagttgcggg atgagcatga
    13681 aaaacagcaa atatacaggg aaagaaagaa actttcagcc catgcgcaag atcatactca
    13741 agacgtacag gcaaaacaaa attatattct tgaggctttg gctcgggtga aagcaaaaaa
    13801 aacatatgaa taaacaaaaa tcacaagaaa ttttcaaacg ttttcaagaa caaaaccctc
    13861 atcctacaac agagttgcaa tatagtaccc cctttgagtt attaattgcc gtcatgcttt
    13921 cagcccaggc aacggacgtc agtgtgaaca aagcgacagc caaactattc cccgtggcca
    13981 ataccccaca agccttactg gatttgggat tagatagatt aaaagattac attaagtcca
    14041 taggtcttta taatagcaaa gcacaaaata tcatgaaaac ctgtgcaata cttattgatc
    14101 agcatgaggg acaagttcct ggccagagag aggcgctgga agcattgcct ggtgttggcc
    14161 gaaaaactgc aaatgtggtt ttgaatacag catttggtca gccaaccata gcggtcgaca
    14221 cccatatttt cagagtggct aaccggattg gcatagcgaa gggtaatgca ccgctggcgg
    14281 ttgagcagga gttattaaaa aaagtgcccc gtgagttttt acatgatgct catcactggt
    14341 taatattaca tggccgttat gtctgtacag cgcgtaaacc acattgcaag acttgcatca
    14401 ttgaagatct ttgcgaatac aaagacaaga atttacttta agacaaagcc ataatgaagg
    14461 aaaatcaaac tataatgagc aatctatgcc tcataaaaat gactggctgt tgtatttcaa
    14521 attcattcta tcaaatcatc cgggttgaga ttagcttttg tacagttaaa acacaaaagt
    14581 tcaattgacc gtcagctctt ataaaaaatt tcaataaagt tcaagaaaag tgctctttcc
    14641 ctctggtatt tcccaatttt tactcataaa atgaaattcc gctgttgcag gagagcatta
    14701 tgaagtggtt ttcatcggtg gtaaatggag tcaaaacaat tgggtcaagc ctgtgggaag
    14761 gttcaaaatg ggtttggaac aacgcctcat tgtcaaaaat cacgactggt gtatttagct
    14821 ataccgcaaa tacaactttt caagtgttag agcaggcaat agccttacgt gatgctgttc
    14881 ccaccttggt caacaatcct caggcgaaga aaattgccta tggaatgggt tatattcttg
    14941 ttaacgatgt tttaccgtta gttgctttaa attttgcaaa caatacggca caaaattatt
    15001 tcggagaggg ttatgatgag gatgcttcat ggtatgcgcc ttattctctt tttttatcgg
    15061 gattagccct aacgaattat ctggtcaaag catacacctg gcggcaagga gttcaaactg
    15121 tagcacatgt agcggttctt gactcattag gcccttcctc atttaattca aataaaaaat
    15181 cacttcctcc ttcaatctgc gatgaattgg attgtaacta taagcgtaaa attaaaggtt
    15241 ggggaagaga gcctcttgtt ttaatgggta atgatgcact gacttacggg gttagtttaa
    15301 ttccttatgt aggtgaacct ctctcgcaag tattaagagt gtatttcaat ggccgctata
    15361 ttattcgttt aactacacca gagctgtgtg agcgccataa atatttggcc atgaaaccag
    15421 agtctgtttt aggatttggg ttaacctttg aagcgacaag tatgttaatg gacaaggctc
    15481 tggaatccac tgtgggcatg cctccgtttt tatattacag ggctctgcgc catttattac
    15541 tgctgatgca tattaatgtt gctgcacata tggagttgcc taaatctgat ttgaaagaca
    15601 aagcctcgtc ttttgatccg tttaatgttt atgaacgcgt caatcgcttt gtagcggatg
    15661 tcatattttc aggtttaatt aaaaggatac ccattgattt caaaccggat aaagatgctg
    15721 agccgttaat tccattatct cctgctctaa aattcggcac tatggtgttg aatatggatt
    15781 tggagaagga aaagaaaaca actcccggac tttttagcaa agccatagat aaagcgtatg
    15841 ttttggtttt acctcctgtt tttcgcggtg ctaatggttt aatcaacgat ccaattgtat
    15901 ttatgtattg gcctactatt cgctcagggg ctatttctgc tgttgaattt gtagagggtg
    15961 tggggaaaac caaaacgatg gctactttgg catgggcacc aaaaactgta gcagccgcca
    16021 tcaatctcaa atttggaatc cctaaaaaag taacacgctt tgttttaatg ttaagcaaag
    16081 aagaagattt ctggaatctg gctacagcat tcaaattgtg gtttgagaga cacaatatca
    16141 aaggtgaacc aaaattaatt accggctcca atctggcttt gaatggggtg acacctcttc
    16201 agcctactcc cgtatctgtt gaaaaggaac ccatagtttc tccccatcta ctaattacgg
    16261 aaaaagatac taaggaagta gtgccagcaa aaagcctgat tcctgttcga tcccaacctc
    16321 agactggagt tgtggtgacg attagccctg atagtttatt ttcaaccagg agacgtcggg
    16381 ttaatccaga tcaagaaaaa gttgcgataa cagaactaag catgactaaa taagttttag
    16441 gggctttttt gacaagaggg caacagaagt gtgttgcccg ttaaagacct tacctagttc
    16501 tatcgatgat attatgcagg ttgtaaatgg ggcgcttgcc gccacttaaa ataatttttt
    16561 tcgtttaaaa aaatacagca tgagtattgc cagaagtacc atggtaacca gaatcatagg
    16621 gtaaccgtat cgccaatcca gctcaggcat aaatttaaaa ttcattccat aaaccccggc
    16681 aataaagctt aaaggaataa aaatactggc aaacaaagtc agaattttca tggattcatt
    16741 catgcgatta tttatagtag acaggtatat gtctaacata cttgccgaca tttctctatg
    16801 caaatcgatc gattctacca ggcgaatgca atgatcatat aagtcccgga agtaaagatt
    16861 atacttttta ttgattaacc ccccatcgtc tgtaagcaac aaatggacaa tatctctcaa
    16921 aggaggaatg atttttctga gggtaatggt tcttcttttg atggtgtata acgcttgtaa
    16981 cttaatcgtt tctggtttat taattaattg atcttccaag gtttcaagca gttgggctga
    17041 agtttcgaca aagttgaaat aatcatcaac aatatagtcc atgatcagat aggtcaagta
    17101 atcacttcca tattctctgg ttaacgagtg ctctccatta aggcgttgat aaatcgatga
    17161 taaattatat ttttcagatt ctctgaatgt gagtaaaagt ttttgcttga tgattaaaca
    17221 gaactgttct gtattataag ataagtgatc gggaggggat tgtaataatt taaaaacaac
    17281 aaaaatataa tcgtctacaa tatccagttt gggtctttgt ctggtattaa gcaaatcctc
    17341 gataaccaag gggtgtatgt tgtattctga gcaaatttta gtaattttct cgatatctgc
    17401 aagacctgaa atatctatcc aaatattatg tccagcctca taggcttctt tcacttgtcg
    17461 ggtatcgaag gaatctaacc gcttataatt ctttttgtca taaatatgta tcgttacttg
    17521 agttggttgc ggtgggtgtt cgcctatata tacggcagtt cccggtaata gtcctgcttt
    17581 tgctgaagcc tttgttttat gagtattcat gaagttatct cagcctattt attttcttat
    17641 taacttatca tttagccatc gtttaagtcg acagttgact actctcgttt atcttgctat
    17701 gcaaagggct ataatgctat atttgcgacc tgattagctt aataaagggc aattattatt
    17761 tgaagcttat agggatgttg ttaatacttc catgtaaatc ataaagaatt aaccccctat
    17821 agtcagtctc tatcaataag gcagaatgag ttgttatata ctatggaagc tcttgtcgta
    17881 tgaatcaatc gttatcaccc caacaatccc ccgggtttct gttcatcgca gtgagtatga
    17941 taacttgcct gatattatta attaacgttt cttttaaaat tattctactc gggggactgg
    18001 tatttgcaat aaacagtttg atttgtcccc ttattgcagc gttattttta ttcgcattga
    18061 gaaattgtac cttcaaagag caacgccatt tacttaatat ttgtttgatg actttatata
    18121 tgttttgtat tggggtttat gtgctcgtca atttacctgc tgctgaatac atgcatgata
    18181 atccagccta tcaagttatt tttgaagaaa tacccaagaa attttttgct acaactattg
    18241 cttttgcact cagtttctat ttgcctcatt tattgttttg tactaaaaat gacagggttt
    18301 tatcaactcc aaagcaatgc gtgttattag ctttactggg gggggtaagt tttttttgcc
    18361 tggattttta tctattattt tctgctccac atgctcatag tttcaagcaa attttcatcg
    18421 attcattcat gatctcgtct cttttactcc taattattgg cgtagtttat ttaacatttt
    18481 tattgaacga caaacaacat aagagcaaag cattaattga aaccacagaa ttaacgcttt
    18541 ttccaattta tcaatatttg atttgcttcg ctgtgaccgt gatgttggtt tgtttggcct
    18601 gtgagtatcg aatcatcgcg atcaataaag acttggtttt atctgccagc tgtattttct
    18661 ttccaattac tatcattatc agttctatag ttggcgaatt gtggggttac caggccaact
    18721 tgaaattagc attggctctt cttgcggcac aatttatttt tgacatgtta ttgatgggga
    18781 gtgttgcact accttctcct ccttttttta atttgaatcc tttttatcat tacatcatgc
    18841 cacgcaggtt atcagctgca tcgttgtcat tgtttgtcac ttttcttggc aatgcaatgc
    18901 tattgcacta tttaaagtat tcaaaatgga aaattaaccg atccttgcga atattaatcg
    18961 ctaatatatc agtcaactcg ttattatgcc tggttgacta cagtttactt tatggcggta
    19021 tttatcctta cgaacaaatt attaatttag cggtgagtgt ttggcaatac aaactattga
    19081 cagcgttaat tgctctcccg gtcattttat ggatgtgcaa gattttagaa agaaattatt
    19141 cattagttgt gcaaacagag aggaattaaa taaacaaatt taatggttca ttctcttata
    19201 ttttttgaag gaatgagata tacactaata gattaagtaa ggtaagtggg gctcccacaa
    19261 gtataaactc cagaaatcca aaacctctga caccacgttt ttcactattt tgaataataa
    19321 taatgttact tgctgccccc aggatagaga gattaccggc aatagtactt cccgcagcca
    19381 ggctcagcag ggttatactg gaatgattat gactcatcaa taaaggcaaa tatattgtta
    19441 ctaatgggac attagagatc aattggctca gggcgatact cattataaaa atcactacga
    19501 tgtggctgac atcaatatga gaatcactca ttccggtttg gaaaaagccg ctatcccata
    19561 cgctttgcat taaaataaag gtgctcgcaa aaaaaactaa agttccccaa tccagtcttt
    19621 ttatataatt ccatcgatgc ttgctaaacc aaattggtac agacgacaag agtgcaatat
    19681 aactaaaatt tagctgaaat tctgaataat gcaggaaatc cgtcaagatt ttgatcaaag
    19741 tcagaactaa catcatgagc aagctcattt taaccaaagt aagattgcta cggtcattta
    19801 tcaaagggag aactggtttt ttaataggtt cttttaattc atttctgtat aaaaaaagaa
    19861 taaaaaaata aactataccc agatttaaaa aagtaggtac ggccaagtgt ttgaaaaact
    19921 caaaaaatgg cgaagccatc tctcccttaa tagcaatcaa taaattttgt ggattgccta
    19981 taggactcat ggtgcttcca atagtaattg aaaatgccaa tgccaacagc aagggtttaa
    20041 ttaaattttt atgtgattga cacagttgta aaataatagg tgtacctata atggcaatgg
    20101 tatcattcat taacaccgcg gagcttaaac ccagaataaa aacaatcaac attaacgttt
    20161 gttttccatt attcgcataa taaaaaatct gatcggtcag aaaagcgagg tagccacttt
    20221 cctctgcggc ttgtgaaatg aaaaaaacac caaacaaata aagcatgaca tccggctcaa
    20281 cggcagtgaa agcgcgtgat ggtgttatct gttggcagag caaaacagca atggctcctg
    20341 ttgccataat tgcccaaatt ggaatgataa ctctgacaac ctgccgcaaa gcaattgcaa
    20401 taagaataat cagaagaatg atgacagcag aaggcatttc tgtcctcttt taattaatcc
    20461 ctctcccttt ttaccggcct tttccagccg tagatggttt tttgtcgtga ttcggttaat
    20521 gttaactcat cgggcggcac attccggcgt atggtgctcc ctgctcctat tgttgcgtta
    20581 gcgcccacgg taactggagc aactaattga gtatctgacc caataaaaac accatcttcg
    20641 ataattgttt gatgcttatt aactccatcg taattacaag taatggttcc agcgccaacg
    20701 tttacatttt ttcctaacag gacgtcccct aagtaactga gatgactggc tttcgtgcct
    20761 tcatcaaaaa tcgctttttt agtttcaaca aaattgccga ttttacaatg ggaagctaat
    20821 tgcgtccctg agcgcaagcg ggcaaaagga ccgatgtggc agtcgtttgc aatgtggcag
    20881 ccttccagta cgctattagc gtaaacttca cacccatcgc ctaatgtgac attagtcaga
    20941 ctgcagtttg ggccaatttt acagccatta cctaaaacta ctttgcctgt aaaaatgcag
    21001 ttaatgtcga tatagacgtc tttgccacaa tataattctc ctctcaaatc aaagcggttg
    21061 gcatcagcca gggtcgcgcc tttttccatg agttgattgg ctgctctttg ttgccaggtg
    21121 cgttcgagtt gctgaagttg ttggcgatta ttaatgccct gcacttcaaa gctgttcttg
    21181 gcggttaatg acgtaattgg tgttttattt tgtacagcca atgagataat ttcagttaaa
    21241 taatattcgc cttgtgcgtt actattactt agttgaggca accatttctt cagattattt
    21301 gccaaagtac aacacacccc ggaataaatt tcttttatat tctttacttg ctcatttgca
    21361 tccttttctt ctacaatgct ataaatttca ccttggttgt ttctgataat acgcccaaga
    21421 ccgcttggat tttccagttc tgcaaccagg agtgctaaaa cagaatgatc tggattttga
    21481 cgttggctgc attcaattaa tgattgaaga gttcccactt gaatcagggg aacatcggca
    21541 gataaaacca gaacgtatgc atcgtcaggg atatggggca tggcttgcaa aacagcatgg
    21601 ccagtcccta gttgctctgc ctgatgcacc caatgaacag gtaagttggg gagtgaactt
    21661 ttaatttgtt caccaccatg accataaatg acatgaattg catcgggatt tagttgctga
    21721 gcggtttcca cgacgtgggt taacagcggt ttgccagcta ggtggtgaag aactttgggc
    21781 gtgtccgaat acattctttt accttgccct gcagctaaaa taataatttg taaattcatg
    21841 agatttgtgt tttcctaagt taaatatcat aatcggtgct gatttaaaca gtctgcccca
    21901 atgttatgaa tgatgtaaag agctatatga taaataagtt gcagttaaag gagtagagca
    21961 atgaatatat ttctaaaggt tttaaagaaa ttaatgaagg aaaaattgag tgagttatcc
    22021 gccaatttga aatggaagtc agaaaccatt gggtataata ccaaattatc aatcgatcaa
    22081 aagaatgtca ttgataaact tgaagaaagt atcgttcttt ttaaagattc aggtaatgac
    22141 gaagcagatt tgctgtcaat caatgcattg atagatactg ccagaataca aattcaatcc
    22201 ataagagaat cctatggtga gccgagagat aaaggggaat ctgtaacctg tctcaataat
    22261 ttgaaaaatc attccaacga ttttcttggc aaactaaaga aatttgattt tgacctgtta
    22321 aataaagctt ataccgaaac acctgaaaat cttgtctatt accatgccgc tatctatctg
    22381 ggagatgaaa tttttagccc tagaacaggg atagattacg aaatccgcac caaaaaagaa
    22441 gtgcaattgg cagtaaggct gcaggcatta agcgagcgaa ttaatccaag ccacaatttg
    22501 gaggaacaaa gaaaaagagc cctgcaagtg ttgcaagatc ttgctcagga taatcaaaac
    22561 gctatcaaga aagataaagg tttttctctt cctggtttaa gcttttgggg tgtttcagta
    22621 gttaccccct cggattggtt tagctcagga gaagggcggt ttggcgttga atttaatacg
    22681 gcggttcgca ccattcaggg aatgactgaa agccagtttg aaagaccgac agtaaaacaa
    22741 acaacggcaa agcaagacga gaccaaacca tccgatgtaa aaaaaacaga ggcaacagac
    22801 gaggaggatg aagaacattc atctgaaaat actcatacaa tgtaagttac tttcaatggg
    22861 tgtattttga tttttctgcc tcgaatgcat acgagaggtc tccaaaagat attttctgtt
    22921 ggagcaagga gatccctcgt tcttaaatct gcagttcacc acacaaattg gtttcaatca
    22981 gttttttgat ttcagggatt tccagattct tgcttaataa atacagcaat ttgcaatgag
    23041 ccgcctcagg tgtcatatca tgaccgctaa tcaagcctgc ttcttttaaa gtatgtcctg
    23101 ttgcgtattg attcatttcc actttcccct ggtggcattg ggtgcaatta ataataataa
    23161 cccccctgcc gcaagcttcc ttaagcaaat ttaaaaatct tggatcattg ttctgggcat
    23221 tacctgcccc ataggtttca aggattaaac cccgcaaagg ttgatttaaa atgtactcaa
    23281 gaacatcaga ggcaaagcct gggaacaggc gaaagtttgc gataaagtga ggagtaatgg
    23341 tttgtagatg gaacggtgcc ttggggcgtg gcaataaacg gtgttgatgt aactcgatat
    23401 cgataccaat cgatgccaag ggtgggtagt tgggcgaatc aaaagcattg aagcgttggg
    23461 cactgatttt ttgggtgcga ttgcctcgaa acaaatgttg attgaagtag atgcatactt
    23521 catggatagg ttgatgagca caaagccata aggaggtaat gagattatct gtcgcatcgt
    23581 tacgaatctc ggataaagga atctgtgaac cagtgactat gacgggtttt cccaaatact
    23641 ccaacataaa tgacaaagcg gaagcagtgt aagccatggt atcagtgcca tgtaatacaa
    23701 caaatccgtc aaactgttca tattcgtgag caatgtctct ggcaatcctg ttccattccg
    23761 tgacggtcat attagaggaa tcaagtagcg gctgatattc tttgataacg tagtcaggca
    23821 tgtcttgatg ttttagagca ggaatgtctg ccagcgcttt ttgaatgaat cccggtgaag
    23881 gctcataacc atgcgatgtt tttatgctac tgattgtgcc gccagtattg ataattaaaa
    23941 ctcgtttttt catgcggatt tataatcaat gatttgttat tgtttcgata agtatagcga
    24001 aaacttggct ataccaataa caactaattt caagacttgc gaaaagcccc aatctctttg
    24061 gtaaaaaagt gtttaattcg gtcatttagt ttatcgaaac tccctcgtta aaaacattaa
    24121 agttacctgt ctccaaggta ttgagagtca ttatgatgag caatttttgt tctataataa
    24181 atcaaaaaat gcaatataaa aacagggaat aaattagtcc aaataatatt tacagattta
    24241 attgtaagct cttgtacttt gtggaaaaat caagttatta acggtttatt ctaagcagtg
    24301 acatcatggt tatcgatcgt gccggatatc gcttgaatgt aggtattata ctcgtcaacg
    24361 attccgatag agttttttgg gggagacgaa gcgggcatga cgcttggcaa ttcccgcaag
    24421 gtggattggc accaggcgag acggccatgc aggcaatgta tcgtgaattg catgaagaag
    24481 tggggctaga taaaggtgat gttgaaatat tgggttcgac caggcgttgg ctaaagtatc
    24541 gacttccgaa gcaatatttg cgtcatggca gtgagccttt ggtgattggc cagaaacaaa
    24601 aatggtattt gttaaagctt gtaacaagcg agcaaaaagt cagattggat ttgagtgatt
    24661 ctcccgaatt cgatagctgg cggtgggtgg attttcatga gcctgagcaa caagttattt
    24721 tctttaagag gcaggtttat attcaagcgt taaaagaatt agaacctttg ttaaaaaaag
    24781 aacgccgtac cccttatggc ttaaaacgta aaagaggtaa tcaaagagct tagatgctta
    24841 aaatacttaa acggatagta caagatgtta ccgctgccag tgatttgaaa gaagcacttg
    24901 gtattttggt gcaacgtatt aatacagcca ttaatgcaga ggctgtttcc gtttatctta
    24961 ttgataataa aaatgctgaa tacgtattga tagccacgga aggtttgaac aagcaggcag
    25021 agtttagagt cagaattgct cttgattgcg gccttatcgg gttgatagga cgaagagagg
    25081 aacctatcaa tatagaaaat gctccctctc atccggattt ttatcataac cctttaattg
    25141 gcgaagatca cctgaacgct tttttaggcg tccctattat tcaacataga aaattatacg
    25201 gtgttttagt tgtacaacag cttgaacaac gtcattttga tgatgctgaa gaatcctttc
    25261 ttattacctt ggcggctcaa ttaggcggga ttattgctca tgcagaggca acaggtgaat
    25321 tggccgagtt aacacaacct aaaaagggta taggagttga caaggtagaa gcagcttata
    25381 ctgcactgac tggaataggc agtgttcctg gtataggaat tggtactgcc gtggttgtgt
    25441 acccacctgc ggatattgat gctgtccctc gtaatccagt tgaagaattg gaagatgaaa
    25501 tcaatgcgtt ttatgaagct ttggaaacga caagagatga tatcaggcgt ttaagtaaac
    25561 gcatgaaaga aactgttgct gaggaagagc atgctttatt cgatgtgtat ttaagaattt
    25621 tggataggga tagcttgggt gctgaagtag agcatgtgat tcgtgaagag aaaatcagcg
    25681 ctcaagctgc ccttgctaca gtgatcaaga aacacgtgca gcaatttgaa agcatgggcg
    25741 atgattatct gcgcgaacga gccagcgatt tccgtgattt gggccgaaga gttttggcgg
    25801 aattacaacg tacccaaaga gaagaaattg tttatccgaa gagaaccatt ttaataggag
    25861 aagaaattac tgctgccgct ctggcagaag ttccagaagg gcaattggct ggtgtcgttt
    25921 cagctaaagg ctccaataac tcccatgtcg ctattttagc gagagctctc ggtgtaccta
    25981 ctgtgatggg gttgagagga ttaaaagtag agttagtatc gcgtcgggct ttgattgttg
    26041 atggctatta tgggcatgtt tatatctctc cttccaaatc attattagcg gaatttaagc
    26101 aattagctca agaagaacag gaattaaacc aaagcctggt aagcttgcgt gataaaccgg
    26161 ccgaaactac tgataactac agagtctctt tgcaagtcaa tactggcttg gcaatggatg
    26221 ccggtttatc aatgagtgtt ggcgctgaag gagttggttt ataccgttca gaagtaccat
    26281 ttatgagtcg agatcgattc ccttcagaag atgagcagac cattatttac agacagactc
    26341 tcaaggcctt tgctccacgt ttggtaacta tgcgtaccct ggatattggt ggcgacaaaa
    26401 ttctacctta ttttccagtt gaagaggaaa atccttattt aggatggcgt ggaataagga
    26461 taactcttga tcatccagat gtcttcctga ttcaggttcg tgccatgatg cgtgccagtg
    26521 aggaactgga taatttacgt atcatgttgc ctatggtcac tacgcttagc gaagtagagg
    26581 aagccgttta tttgattgaa caggcatttg aagagctggt ggaagagggg tgcgaaatag
    26641 taaaacccaa aattggtgtg atgattgaag tgcctgccgc tgcttatctg gcaagagaaa
    26701 tggcgaaacg agtggatttt atttcggtgg gtagtaacga tcttactcaa tatttattgg
    26761 ctgtggatag aaataacgcc cgagttgccg cactttatga tcccttgcat ccagccatgt
    26821 tgcgtacatt gctaaaaata gtcgaaggag ggcatgcagc tggtgttgag gtgagtattt
    26881 gtggcgaaat ggccagcgat cctttagcgg tgatcttgtt aattgctatg ggttttgaca
    26941 ccctaagcat gaattccgcc agtttaccca gggtgaaatg ggttattcgc aattttgcta
    27001 tcgcgaatgc ccgtaaaatt ctggcagaag ttttggaatt tgaacatcct gctgaaatac
    27061 gatttcattt gcaaaaggct ttagaagagg aaggattagg gggattaatc agagcaggaa
    27121 agtcatgata tccggtgagt tactgattaa tggagtgatt tacatcatta ttggtgcttt
    27181 tgcaggaata atggcgggtg ctctgggcgt tggtggcggg cttattgtgg taccaggact
    27241 cgtctatatt tttcaacact cgcaggtgat tccagaagat atccttatgc atgtcgctgc
    27301 aggttgttca ctggcggtga tgatttttac ttcttatgca tccttaaaag cacataataa
    27361 aatggatgag atactcttac ccctttttaa gaagttatgt ccgggtttgg ttcttggtac
    27421 tattttaggt tccataatgg cttcttttat tccaacccat ttattgaaaa taatatttgg
    27481 gctgttcttg cttggtgtag ctatgaaaat gcttcttgat atccgagtga gccacactga
    27541 taaatttccg ggatattggc ttaatacttt agtcagtagt tcaataggtt cgatatccgg
    27601 tttattaggg gttggaggag gagtcatcat tatcccttat ctgacctatt gtggagttcc
    27661 agtcagaaaa attgccgcag tatccaattt gggcactttt gcagttggtc tagtgggtac
    27721 aattatgttt atgattactg gcacattaga catgacaaaa atatcctatt ccacaggata
    27781 tatatactgg ccagcagttt taggggtagc aattccaagt agtttgattg caccatttgg
    27841 ggcgaaatta aattatcttt tacccgtaaa ttatttaaga tatggattta tcgtgatttt
    27901 aattaccacc gcaattcata tgtttttgtg aaaggaattt gaataaccat gctcacctat
    27961 ccaaatatta atccaattgc tttttcttta gggccattaa aagttcattg gtatggtttg
    28021 atgtatttaa tcggttttat tggcgcctgg ctactgggtt attggcgcat taaacattat
    28081 aaattgaact ggaataacga ccagttaagt gatttgatct tttatagtgc ccttggtgtt
    28141 attcttgggg gacgagtagg ttacatgttg ttttatgaca ttcaggagtt tatccaccac
    28201 ccctgggtat tatttaaaat atgggaaggc gggatgtctt tccatggtgg tttgcttgga
    28261 gtggtaattg ccgcctggct cttttgccgt aaatacggaa aaactttctt ggaggttgga
    28321 gactttgtcg ctcccctagt tcctttaggt ttagcggcag gtcgcctggg aaatttcatt
    28381 aatggggaat tatgggggcg tgtcactgat gttccgtggg gtatgatata tcctcatgtt
    28441 gatgatcaac caagacaccc ctctcaattg tatgaatttg gtttggaagg ggtcgcttta
    28501 tttattttga tttggtgtta tgcgtcaaag ccgagacaac aagggagagt gtgcgcttta
    28561 tttttaatgg gatatgcgat ttgcaggtta attgctgaaa gctttaggca acctgattcg
    28621 caattaggtt ttgtggcatt tggctggctg acaatggggc aggttttatc catccctatg
    28681 ttattaattg gaatttggtt atggtgggct aagcgatgaa aacatatttg caattgttgg
    28741 agcatatttt acaacaagga gtagaaaaat cggatagaac gggcactgga acattatctg
    28801 tatttgggta tcaaatgcgt tttgatttga ccaagggatt cccgcttgtc actactaaaa
    28861 aactacacac gagaagtatt gttcatgaat tactatggtt tttgcgtggt gataccaata
    28921 tttcttatct taaggaaaac ggggtaacta tttgggatga atgggcagat aataatggtg
    28981 atttggggcc tgtctatgga aagcaatgga gaagttggcc aactgctgat ggacacacta
    29041 ttgatcaatt aagtgatgta gtgcaacaaa taaagagtaa ccctgactct cgtcgcttga
    29101 ttgtaagcgc ctggaatgtg ggtgaattgg ataaaatggc tttaatgcct tgtcatgcct
    29161 tgtttcagtt ttatgttgca aacaacaagt tatcctgtca attgtatcaa cgctcagccg
    29221 atgtattttt aggagttcct ttcaatatcg cttcttattc gttgcttact catatggtcg
    29281 cccaacaatg taatttggat gtggctgagt tcatatggac cgggggagat tgtcatttgt
    29341 atcttaatca tttggagcaa gcgcaaatcc aattaacaag agaacctttg ccattgccct
    29401 ccttaactat taagagaaaa ccagcttctt tatttgacta tgcttatgag gattttgaat
    29461 ttttgaatta tcaatcacat cccgcaatta aagcacctat tgccgtttaa ttattatcag
    29521 cattggattt ttattgctaa ttatggttta atattgcctc aagtgtttat ttgaggccca
    29581 ttgctgtgaa aaaaattgta gtgtttgtct tgctggctgt tgtttgtagc gcaggctttc
    29641 cgagtaaatt gagtaaattt ctgaataaaa tggatgctga gcaaaaacaa agagacgctc
    29701 aggaatggca acaagatatg aactttggag attatgcttt tcgtttgaaa gagcgctata
    29761 tcgatgatcg cggacaacgt tgccgtgatt acgaattcag aggacgctcc aacccttatc
    29821 gacatggtta ttttactgtg tgtgatgatc gttgatttgg tcgaaaatac tctttctgta
    29881 tattaaatta tcgtccgctc cctgacggtc gcggctcagt tttagttata atctgagccg
    29941 cgaccgtcag ggagcgattg tccgaatagg ccctacacta aattcctaat aatatcaggc
    30001 aaaggcacgg atttattttg ggtataatca atccaaacta ttttactcac cccttgtgcc
    30061 ataagcgttt cttcctgata caaatcatga tccataatca tgctggaatt gccaagacta
    30121 tgtaccttgc tgtgtatggt tacagtggca ggataaacga ttggtttcag aaaggtacaa
    30181 gccacatgaa tgacaacagg acctgtttgg cctgtcattt tgatgtcaag ctctcggagc
    30241 caatcaatcc gggcttcctg aaaatagtca aaataacgag cattgttaac atgtcctaat
    30301 gcatccatat cgccccaagc gatatcaaaa gtttttttat gtattatttt gtttatttca
    30361 gtcatgataa gtccatagga tcaacgtcca cattccagcg gatgccatta cttaatttgt
    30421 tcattgtcaa ccactctctt aattgcgtca atgaactttt tagaactttt cttgaagggg
    30481 atttaattaa tagttgcatc ctatgctggt tagcttttcg tggcaaagga gctggcgcag
    30541 gacccagtac cgtgagagga tgagcctgaa tttgatcttt ggttgctttt aggaattgat
    30601 ccaccttgtt gacggttttg ccctgagctc tgatgaccgc taaaaactga aaagggggca
    30661 tttcagcctc ttgtcttgaa attaataaag cgtttgcaaa ttcatcatac ccatgctgaa
    30721 tgagtagatt aagcagggga tgatcaggta aataagtttg aatcaatact tgcccgggat
    30781 gttctgctcg cccagctcgt ccagatactt gagttaataa ttgtcccaaa tgttcaatag
    30841 cccggaaatc ctggttgtaa aagcctgcat cagcatctac gacaacgacc agggataggc
    30901 gggggaaatg gtgccccttg gctagcattt gagtgcctat gatcagctga gcttcccctt
    30961 tattaatttt gtccaaatgg ctgtttagag aatttttttt acgaacctca tctcggtcaa
    31021 ttcgtaaaac atttgtatct ggaaactgat ggcttaaaaa ctcataaatt ctctgggtgc
    31081 cagatcctac ggggattaat tccttgcttt ggcagttatt gcaaaacaaa ggtattcttt
    31141 gggttaatcc gcaatgatga caaatcattt gtcctgcctg tttatgcaag gttaaatggc
    31201 tatcgcatgc tttgcaatca accatccatc cacattgatg gcaaagtaac actggggcaa
    31261 agccgcgtcg gttaatgaaa acaagaacct ggttttgttg cagcaaatga ttttctatga
    31321 ttttaagggt tgggagagcc aagccatgct gtaccatttg cgatcttaaa tcaatcagtt
    31381 gatagtgtaa tggtgtagtg gaggcagctt tatgcgttaa gcgtaataaa ttatattttt
    31441 tttgtttgca gttgtggatg gtttccagac tgggagtggc tgtaccaaga atgataggaa
    31501 tattggcaag atgagcacgc attaaagcag tatctcgcgc agaataacgc accccttcca
    31561 tttgtttgag cgaactgtca tgctcttcat cgatgataat taaaccgaga ttgggcagag
    31621 gggtaaaaac ggcagaacga gttccaatca ccattttaac tttgttctct tttgccagct
    31681 gccaggcaat ttgcctttct gattcattca aattggaatg taatacggtt atgggatggt
    31741 taaatcgggc tgtaaaacga gataaaagct gaggggttaa cccgatttct ggtaccagca
    31801 ccaatacttg tttgccttgc tccaatattt tggcaatgac atgcaaataa acctctgtct
    31861 taccacttcc ggtaacacct tgcagtaaaa agcattggta atgatggatg tgttctaaaa
    31921 tggaagctac ggcaacggct tgttcaggat tgagtgttaa cggtggactg agtttttcag
    31981 gaattttggt tggcaaggta acttgctgtg acaggttaat ggcttgacaa gcaagtagtg
    32041 tcgcaatttg atttccgttg tagccatgtt gttttagggt ctgtttggaa attcctttct
    32101 ggtgtctgct taaaaaatca atcaattcat gttgtttttt tgcttttttc gagatgagtg
    32161 tcttcgcctt ttcaacaggg atattcaatt gataaaaatc ttctgtaggt agtgagcaag
    32221 gcaaacccag gcgatatttt ttgggtatta ccaaaggaat cacttcagat aatggtgact
    32281 gataataact tcctacccat agacaaagct ctaagagatc ttcaggaacc agaggtttct
    32341 catcgattat tgaagtgatt ttctttaacg aatggcccgt ttgctggcca caaactttat
    32401 tgattacaat gcccactcgg gcttgattgc gaaaaggaac ccatactctt ccaccaatgc
    32461 atggagttaa atccagggct tcgtaatcaa aacaatctcg attggtatgg gggatacaaa
    32521 cttgataaat atcgttcatt taacttctaa gccattcatg actcgctgat tggccgggcg
    32581 cggaaaacac tttgactact actttttcga tgcgatgttt atctaactcc tctttttctt
    32641 taatcagttc attaacaaac cagcgtgata actcttccac ggtaatattg gttaagggca
    32701 ttaaggtgac atcttcttta agaaaaggga tttttttctt attgaagatg aaataataat
    32761 attcttcgtc ttccgagtat tctaaaaaag gtgaaaattg tggcataaga aaagtttgat
    32821 ttaagtagcg gcataattta tgaatccgct ctttgtaata acggtaatca aaagtcatac
    32881 cattttcttc tacccaggta gttagagcca
//
LOCUS       DAXLDT010000018        32517 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXLDT010000018 DAXLDT010000000
VERSION     DAXLDT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32517
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.018"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(209..649)
                     /locus_tag="ACF2SP_000688"
     CDS             complement(209..649)
                     /locus_tag="ACF2SP_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFD2358493.1"
                     /translation="MAENTTESNVIYFTRLHWIIFFGPFVGLGLVMAIYIYIIQLREV
                     SLFLGFFALIWILITWVTYHFSSITIKRKQVILRTGIIVRQTVDIPLNKIETIDIRQS
                     ILGSILSYGTLIITGTGGTRYLINYLYKPLTCRRYIEQLMNEPH"
     gene            780..1202
                     /locus_tag="ACF2SP_000689"
     CDS             780..1202
                     /locus_tag="ACF2SP_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFD2358494.1"
                     /translation="MKSISQCLNKQLSTICQHSMQLEELSLKISAFLPSHFISKCQVG
                     SFNKGCLVLTTTNAAFASEIRYLLPELRDKLRKEAGLYQLSSIKITIIAPSSQIENPA
                     QKKSLTLSEKAKATIISESQHCTYEPLKKALLHLAQSE"
     gene            1220..2524
                     /gene="lem28"
                     /locus_tag="ACF2SP_000690"
     CDS             1220..2524
                     /gene="lem28"
                     /locus_tag="ACF2SP_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem28"
                     /protein_id="HFD2358495.1"
                     /translation="MCSEFFFTIMLKYSTNMLELAMPKINKIVNGTDLTPHYLSEPNK
                     EFKIYRYNKEVYAVRFENDEPMDYVLMWKSHKSGHTQKSEMIKNTEEDYKELGKGEQG
                     TVYEKTEDKAMKVSRGRHPREFYEEINLHIIEQQFFLKYHGIQEHFVLGLWNIKNEEN
                     VYFYMPKINAIPINKKIDQPKIEEFVLALKELNDAGYWHPDLANNPYHISPQNLIATE
                     EMVKTIDLDGGFRYDKGRVDELSRKSLVYGKDQWLYVYNFIYPPTDEEDNRIDWKVPI
                     EKWYENNRDESLSDNPHTLLRFYHEGLIALPKKLAHDLHETILEELSQDKKFKKHSLE
                     VGVNRFFSPKKQGIYEGLKGDGLKKVILKELRDSLTEIRTMEQLEEKKLEFLASPEMQ
                     ILAEGQDKTTKALNLKTSSRKKVMVIFKEAEERILNSPNVSI"
     gene            complement(2653..2778)
                     /locus_tag="ACF2SP_000691"
     CDS             complement(2653..2778)
                     /locus_tag="ACF2SP_000691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358496.1"
                     /translation="MHLNRLEANIDDGRIASKEMLAKEENSEGPFLWGSYQWSFT"
     gene            2825..3622
                     /locus_tag="ACF2SP_000692"
     CDS             2825..3622
                     /locus_tag="ACF2SP_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2358497.1"
                     /translation="MLKQIVLTGLVIIGIVITLMYLFQRHLIYFPNRHTPKLEDYNAS
                     DMKVVSLRTKDNLHLKSWYKPASKHRPTILYLHGNAGHIGYRMPLVREFIDAGLGVFL
                     LEYRGYGGNPGKPSEKGLYADGETAIEFLIQHGVPSKRVILYGESIGTGVATHLATKY
                     PVCAVMLQSPFTSLTRLAQYHYPLNFLKPWDQYNSLARMKKINAPILVLHGKLDQIVP
                     YQEGLNVFNEANEPKKMISFDDKEHNDLWSADNFSREIIQFALNQCS"
     gene            complement(3677..4093)
                     /locus_tag="ACF2SP_000693"
     CDS             complement(3677..4093)
                     /locus_tag="ACF2SP_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358498.1"
                     /translation="MSFSLSELLHYKNEKIVTHFCYSHPEYSIQEGLFLFEDLLAWMW
                     LNKQRRLRGKKTYLFGPLLILDEMWHSFILHTRDYIDFSMKYFGEYFHHDVEPVGFEH
                     VLDEEELADYLQDCFQYLNKEWVERRFSMAFAEMDD"
     gene            complement(4208..5059)
                     /locus_tag="ACF2SP_000694"
     CDS             complement(4208..5059)
                     /locus_tag="ACF2SP_000694"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFD2358499.1"
                     /translation="MCRFVAYLGRPTLLENVLVRPKNSIIMQSLHARETKYRTNGDGF
                     GLGWYSPEVSSDPALFTSVYPAWNDRNLLHLTAKIQSPCFFAHVRSASAGGVTNYNCH
                     PFIYGDWMLMHNGEINDFIDVKRHIRHLLDDDIYHWVKGQTDSEHLFGLFLQLAKGRD
                     INQLSVAADVLQETINKILEVIGQFGHSGPSYFNICLTNGKKIIATRYCSHEKKKPLT
                     LHYLLGYVLSTQGLWIKEEGNPAYVVVSSERLNDLDEGWEDVPPQHMILIDENKNIQL
                     RPLQTLG"
     gene            complement(5213..7249)
                     /locus_tag="ACF2SP_000695"
     CDS             complement(5213..7249)
                     /locus_tag="ACF2SP_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HFD2358500.1"
                     /translation="MKFKIATCIVTMLSSFIVFSSNSNFSDNNTNDLLHLDWRDTSVS
                     PAVDFYTYANGNWKKNNPIPADYASWGSFYVVNEKVQNIIHQMLINASKNTHAKPGSI
                     EQKVGDFYFSGMDVDSINKSGITPLKPEFTKIEEIKNLKDLQAEIAHLHKIGVGPLFN
                     FGSMQDFKDSTSMIGAVMQGGLGLPDRDYYLKKEAKFKQVREAYVNHLTKMFELLGDS
                     PEMAAKEAKIVMDIETQLAKASMSQVEQRDPHAIYHMMTITELDKLTPNFSWSQYLSA
                     RGQGKIKKINFAMPDFFKAMNELLQSVSLDEWKIYLRWHLIDAFAPYLSKPFVDQNFK
                     MVSALTGTQKLLPRWKRVVNTENGALGFAIGELYVEKYFSPESKQKVLDILKNIRAVL
                     QEDIKTLSWMSPKTRDAALKKLDLMEERVGYPTKWWDYSSLKIDRGPYVLNVIRANEF
                     LINRDLDKIGKPVDRSEWAMTPQTINAYYDPSMNNLNIPAGILQSPFFDPKAPTAINY
                     GAIGYVIGHEMTHGFDDQGAQFDGYGNLKNWWAPNDLSKFKNATQCIANQFSKYVVDG
                     DLHVQGQLVVGEATADLGGMILAYKAFQRSKEYKNAKTIEGITPDQQFFLGTAHVWAM
                     NVRPEQLRNQVTTDPHPPAVYRVNGSLANIPQFQEAFNIPNGSPMISKNRCIIW"
     gene            complement(7393..8307)
                     /gene="lpxC"
                     /locus_tag="ACF2SP_000696"
     CDS             complement(7393..8307)
                     /gene="lpxC"
                     /locus_tag="ACF2SP_000696"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFD2358501.1"
                     /translation="MIKQRTPKKVIQATGVGLHSGEKVLLTLRPAPVNTGIVFRRVDL
                     SPVVEIPASYEYVGDTMLCTTLHHGKVKIATVEHLLSALAGLGIDNAYIDVNAPEIPI
                     MDGSAAPFVFLIQSAGIREQNAAKRYIRILKPIRVEENGKYVQFLPHKGYKITFTIGF
                     EHPVFNDRPQTVSFDFSGTSYVKEVCRARTFGFLSDYEKLRECDLAKGGSLDNAIVVD
                     DYRVLNEDGLRFESEFVTHKVLDAIGDLYLLGSSLIGAFEGYKSGHELNNRLLRELMV
                     RQDAWEYTYFDTENYLPAVHPEYYPVEA"
     gene            complement(8555..9751)
                     /gene="ftsZ"
                     /locus_tag="ACF2SP_000697"
     CDS             complement(8555..9751)
                     /gene="ftsZ"
                     /locus_tag="ACF2SP_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFD2358502.1"
                     /translation="MFELMESSQQENNAVIKVVGVGGGGGNAVEHMVAENIDGVEFIC
                     ANTDAQALRGSSAKIHIQLGDALTKGLGAGANPQIGREAAEEDREHIKEILSGADMVF
                     ITAGMGGGTGTGAAPVFAEIAKELGILTVAVVTKPFSFEGKQRALAAEEGIRRLAEHV
                     DSLITIPNNKLLSVLGKNISLLNAFKAANNVLLGAVKGISDLITRPGLINVDFADVRT
                     VMSEMGMAMMGTGSAVGEQRARQAAEAAIASPLLEDVNFSGARGILVNITAGLDMSIG
                     EFEEVGDVVKEFISDDATVVVGTVIDPEMTDEMRVTVIVTGLGDNRQRQQQPQQPLRA
                     RLVETTRSDGSLDYQQLERPAVIRKQAQTGTATTATKQTNNGNESVPDVDYLDIPAFL
                     RRQEEA"
     gene            complement(9946..11184)
                     /gene="ftsA"
                     /locus_tag="ACF2SP_000698"
     CDS             complement(9946..11184)
                     /gene="ftsA"
                     /locus_tag="ACF2SP_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFD2358503.1"
                     /translation="MAKKIEKNIITGLDIGTSKIIALIGEVTSDGTIEIIGIGRHPSR
                     GLKRGVVVDIETTVNSIQRAVQEAELMAGCEVRTVYAGIAGSHIRSLNSHGIVAIRDQ
                     EVSQADVERVIDAAKAVAIPADQKILHILPQEFIIDHQGSIREPIGMAGVRLESRVHI
                     VTGSVSAAQNIVKCVRRCGLDVIDIILEQLASSHAVLTEDEKELGVCLIDIGGGTTDI
                     AIFCEGAIQHTSVIPIAGDQVTNDIAMALRTPTKAAEAIKVNHACALPELANPNQMLE
                     VTSVNDRPGRKISAKALADVVSARYEELFSLVRNELRRSGFEDRLAAGIVLTGGASNV
                     RGAIELAELCFEMPVRKGCAHYVSGLTEATENPSFATGVGLLLHGFQQQYESGYNVPA
                     MNDNGKSIWSRMKEWFQGNF"
     gene            complement(11184..11903)
                     /locus_tag="ACF2SP_000699"
     CDS             complement(11184..11903)
                     /locus_tag="ACF2SP_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFD2358504.1"
                     /translation="MEVNSVGLRYLTIMGLLILSALLLAGRLGYLYLADAERFPITTI
                     KVAATYEHITHKELENVLAKYLDASFFLLSVKGLQSELNSMSWIDTAYVERVWPDTLK
                     IKLTEKKPVAIWGDALMTRDGKLFNQGSVPSDLDIPKLKGPQSQQLEVLQVYEKLSKI
                     LSSYGLNASGLYLRDNQSWVLLLNHSVKIYLGKKELEERLLRFCKAYPAVFAEKADQL
                     AGVDLRYPRGMAVQWKQQMGR"
     gene            complement(11919..13013)
                     /locus_tag="ACF2SP_000700"
     CDS             complement(11919..13013)
                     /locus_tag="ACF2SP_000700"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HFD2358505.1"
                     /translation="MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAM
                     DKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYED
                     GCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
                     QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVG
                     REIELAVLENSIPCGKPRVSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSL
                     EEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQAT
                     GVAYPDLLDELINLAMVHHNCRQHLVTNYL"
     gene            complement(12997..13923)
                     /gene="murB"
                     /locus_tag="ACF2SP_000701"
     CDS             complement(12997..13923)
                     /gene="murB"
                     /locus_tag="ACF2SP_000701"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFD2358506.1"
                     /translation="MSITGMDSSHVTESQGTLLFNEPLAEYTTWRVGGPAARLYKPAN
                     IDDLALFLSRLPFDEPLLWLGLGSNSLIRDGGFSGTVILTQGCLKEMTLLSDNCIRVE
                     AGVSCASMARFSARNNLSEGEFWAGIPGTMGGALRMNAGCHGGETWQSVIEVQTINRR
                     GEIRTRKPEEFEVAYRHVAGLGDEWFISAKLQLTPGNKETSLQLIKDLLAHRAKTQPT
                     NEYNCGSVFRNPPGDFAARLIESCGLKGVSIGGAVVSEKHANFIINHQGTATAANIEA
                     LIHLVQTKVREQTSIELIREVHIIGDANVQTR"
     gene            complement(13932..15341)
                     /gene="murC"
                     /locus_tag="ACF2SP_000702"
     CDS             complement(13932..15341)
                     /gene="murC"
                     /locus_tag="ACF2SP_000702"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFD2358507.1"
                     /translation="MNNSEQFLSPRMGRVEQIHFVGIGGAGMCGIAEVLHNQGYRITG
                     SDLGESGTVQRLRSLGIQVYIGHRLENIKGADVVVRSSAVDFNNPEIVAARELMIPVI
                     PRAAMLAELMRFRHGIAIAGTHGKTTTTSLVSSLLAEGGLDPSFVIGGKLNSCGANAQ
                     LGKSAYFVVEADESDASFLFLKPMMAVVTNIDADHMDTYEGDFEKLRTTFLEFLHHLP
                     FYGLAVVCLEDEEICRILPAIQRPTLTYGFKEEAHYRAINWTQKGMLSEFVVVRPAPH
                     KQLTIQFQYPGRHNVLNALASIAIATELGVDDDSIVRGLQKFQGVGRRFQMLGEKQFE
                     KGAAIIVDDYGHHPQEILSTIDAFRRVWPERRLVHVFQPHRYTRTQSLHRQFVDVLSL
                     SDELLLMDIYAAGETAIPGVTSENLANEIRSRDKRVTIVSEQSLKATLDEFIKDGDVI
                     LMQGAGSIGQMAVNLMKNM"
     gene            complement(15351..16526)
                     /gene="ftsW"
                     /locus_tag="ACF2SP_000703"
     CDS             complement(15351..16526)
                     /gene="ftsW"
                     /locus_tag="ACF2SP_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HFD2358508.1"
                     /translation="MRPRHLNQRGKPVSRPISLYDKWLIGAVFGLLIIGLMMVASSSV
                     MISTKYFHQPFHFLIRQACYLFVGLLLALIVVRTDSSFWEKISMPMMIGCVFLLLIVL
                     IPGIGKSVNGSRRWLALGPIGVQVSELTKLAMIFYLSGYLVRQQEAVCESIFGFIKPM
                     AILAVVSVLLLLEPDFGATVVISGTVMAMLFLAGVKLRYYFGLMLVVVTALALLAVSS
                     PYRVARLTAFLDPWADQYNSGYQLTQSLIAFGRGGWFGTGLGESIQKLLYLPEAHTDF
                     LFAVIAEELGLFGILVVITLYSILVIRGLNIGYTAYTQERHFASYTAYGLTIWLALQA
                     SINMGVNAGLLPTKGLTLPLLSYGGASMVINCIVIALLLRIDHENRWQSLGLKPLTA"
     gene            complement(16532..17875)
                     /gene="murD"
                     /locus_tag="ACF2SP_000704"
     CDS             complement(16532..17875)
                     /gene="murD"
                     /locus_tag="ACF2SP_000704"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFD2358509.1"
                     /translation="MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEF
                     QNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTPVLERARQAGASIYGDIECLARE
                     ISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
                     LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVY
                     TVPHQSCQTDIKCISFGKDAPSMGNWGLIEQENTTYLAKGMERLLPVESILIKGVHNW
                     MNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVGWINDSKGTNIGATI
                     SAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKV
                     VPVVRASSLEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL"
     gene            complement(17889..18974)
                     /gene="mraY"
                     /locus_tag="ACF2SP_000705"
     CDS             complement(17889..18974)
                     /gene="mraY"
                     /locus_tag="ACF2SP_000705"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFD2358510.1"
                     /translation="MLYWLTQLLQGQYHAFRVFQYLTFRSILASLTALIVGLLCGPLM
                     IRWLRGLQIGQMVRSDGPQTHLSKAGTPTMGGVLILLAITVSCLLWCDLRQTSLWLVL
                     LVTLANGLVGWVDDYRKLVLKNSKGLPGRWKYFWQSVIALVAVSYLYWNASLPVHTQL
                     TVPFFKTVTWDLGVFFPVLAYFVIVGSSNAVNLTDGLDGLAIMPIVMVAGALGVFAYA
                     SSNAVYSNYLGIPYVPNTGELTIFCSSIVGAGLGFLWYNSYPAQVFMGDVGSLALGAA
                     LGIVAIVVRQELVLLIMGGLFVIETLSVILQVGYFKYSGGKRLFRMAPLHHHFELKGW
                     SEPKVIVRFWIITVVFVLCGLATLKLR"
     gene            complement(19089..20426)
                     /gene="murF"
                     /locus_tag="ACF2SP_000706"
     CDS             complement(19089..20426)
                     /gene="murF"
                     /locus_tag="ACF2SP_000706"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFD2358511.1"
                     /translation="MNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
                     DGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIAL
                     TGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPG
                     EIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAY
                     AHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLH
                     NVRNALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRS
                     VLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGIAARQLGIDKLMSCGLHSELA
                     SQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG"
     gene            complement(20667..21446)
                     /locus_tag="ACF2SP_000707"
     CDS             complement(20667..21446)
                     /locus_tag="ACF2SP_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFD2358512.1"
                     /translation="MQANWSLILNVLLLIGVLVAIGRLMKTRRQSLNQEHYQPSLGQV
                     ENNSYGSQSYNDEIIAVRKVNREVPLDFENFDGSEKRQAIRKPSVKTEQPQLIPEEEE
                     CESNERTEPNSDCSSSTAMVFLLAKENRQFAGYELLQTVLAAGLRFGERHLFHRHQFT
                     NGQGPVLCSLAAATATGVFDLQNIGAFSVRGLCLFMQISNNPEINAERFSVMLDTAKQ
                     LSEGLDAYLLDDQRKPLTEERIARYHRYLKIEHLMDDCVAN"
     gene            complement(21451..24945)
                     /gene="smc"
                     /locus_tag="ACF2SP_000708"
     CDS             complement(21451..24945)
                     /gene="smc"
                     /locus_tag="ACF2SP_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFD2358513.1"
                     /translation="MHLKQLKLAGFKSFVDPTTVHFPSRLVAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDIIFNGSSNRKPVGQASVELVFDNSLGRLTGPFASYGEIA
                     VKRVVTRDGESFYYLNGARCRRKDITDIFLGTGAGARSYSIIGQGTVSRLIEAKPEEL
                     RAYLEEAAGVSKYKERRRETLQRIEQTRDNLTRVADIREELGKHLQRLERQAKAAERY
                     TILKEEEKICRAEIQALKWQEYSEKQTVKQRELQELAIRNEQQQSLLTSCYKERAVLN
                     EQLQDAIDQSQSIQDSLYQSGTEIARLEEAIQQQEREKKRLENDRQQMQVDSQLIEEQ
                     LKLDNEELHNCKLKAQQLEEHLHHLKMEFKEQESLWQEKQKEQSQWEKIWQEVQINSN
                     TIKNEYQLTQAKLQHLEQKRHQVLVRLEKLNLEQSAISLDDLQKNKIKLQEKHFQLKE
                     TQEFDEEQLRQANETQSDLQNQLQNTEQQLHALQDEFHRLNSELAALKAAQIAAKKEN
                     RPNKDGIAEWSDKPRLMDVLKVEPQWQSSCEIVLGDDLIAYVLDDFAELWSRWQFCED
                     HKESVVTMRALNGEKQERPRLADKIQGLVPSTVNHLEHIYAADNLEEAKAWFPDLLAH
                     ESIVTVNGIWLGKGWIKLPRQKEQDEVGVLARQQRIVELNLSVESLQQKIESIRLARD
                     SYQARLQENLNFIKLHQLNLNASNEALRNHIIAVNSQEQAIFHAEKQKDAITNEIIEL
                     ESSLEELNAEQLEMADKIRELEGQYQASELQFKRFINEKDTWNSVLVEQNKQVESVRA
                     LLHQSELEYDREVTKIQQITDRISREKERLQRAQMRLEELTQHYLQSVAPEAEFKNQL
                     AALLIKHGEIESMLTLSREKVSQLKLQLEDQEKLILNYDQEVKRIQELMNQIQMEKQA
                     LSVRASSIQETLDEANLQAQSLLERIPSGVTQSIKEDELIAILEKIKRLGAINLAAIE
                     EFAAEQQRKVFLDEQYNDLSQALSTLETAIEKMDNETKSRLENTFDEVNTSFKALFPR
                     LFGGGRAQLELTCDNLLEAGIVVMAQPPGKRNSSIHLLSGGEKAMTAVALVFAIFQLN
                     PSPFCMLDEVDAPLDDLNVSRFCDLVKEMSQFIQFLFITHNKVTMELAEHLVGVTMRE
                     PGVSRLVAVDVKQALTME"
     gene            25120..25800
                     /locus_tag="ACF2SP_000709"
     CDS             25120..25800
                     /locus_tag="ACF2SP_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HFD2358514.1"
                     /translation="MKQQFYKLPQILISLCLLLILSTPIQAEPPNLSLIRKEIKNYYD
                     SGLYYHELERTIKLAQEYIHQQYLINKNNKHPQKLAIVLDIDETSLSNYDKMVKRDFT
                     GSKEQIHKEILAANSPAIKPMLTLYKNALKQGIKVFFVTGRQESERDATRANLIKAGY
                     TKWAGLYLRPNGYSSPSIIPFKSKAREMIAKKGYTIIASIGDQCSDIQGGYAKKGFKL
                     PNPFYYLP"
     gene            complement(25902..26963)
                     /locus_tag="ACF2SP_000710"
     CDS             complement(25902..26963)
                     /locus_tag="ACF2SP_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFD2358515.1"
                     /translation="MSKLINLAVLSFSSSLAFAGPLTLKGEVDVHITPKNPSGVAQSL
                     TFKLPKYELSTEAKSYLREQLSEYPKNSINTASFSSELPRKVKLGMQLTPVLDQGYHG
                     SCVTFAVTAAIDAALGAGDYISQLCNLELGSYLAIHDKAKASGWNGSFGYWVLQQISE
                     YGIISQNYQKLNGCAGVREYPLEDESNEGKPMSDSEFLAHSVPVSNLISWEALLKDEE
                     SFSAKADMNQIVYQIKEELAKGNRLTIGMLLDVFVGDAGAVGTNRAYNDTWMLTPEIV
                     LDAMNGMIYAGHELVITGYDDDLEVMDEEGHVNKGVFTLRNSWSKFAGDQGDYYVTYD
                     YVKFLAMEVMAIRMKEKAA"
     gene            27273..28085
                     /locus_tag="ACF2SP_000711"
     CDS             27273..28085
                     /locus_tag="ACF2SP_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HFD2358516.1"
                     /translation="MRTLLSCFLITLLSFGLIINEASAKRFGGGRSFGVQRSQSSLFS
                     SNKTHNTSSLTQKANKSRWGGVLGGLLVGGLLASLFMGNGLANGLITWLILGSVIFFV
                     VSFLRRKMNPGFQATQSGAFRQNSFEQFTQNYGNRNSGGAHFAQQETSFDADNFLRDA
                     KVTFIRLQAAYDQKNLQDLQTFTVPEVFAEIKMQLDERGDAPNQTEVISLDAKLLDVS
                     KQSLSTIASVQFTGTIKENGEISNLDEIWHFRQFDNRKEWVVGGIQQEVFQP"
     gene            complement(28159..28641)
                     /gene="greA"
                     /locus_tag="ACF2SP_000712"
     CDS             complement(28159..28641)
                     /gene="greA"
                     /locus_tag="ACF2SP_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFD2358517.1"
                     /translation="MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKE
                     NAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSE
                     LTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
                     "
     gene            complement(28650..31853)
                     /gene="carB"
                     /locus_tag="ACF2SP_000713"
     CDS             complement(28650..31853)
                     /gene="carB"
                     /locus_tag="ACF2SP_000713"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFD2358518.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGTQAVRALKEEGYRV
                     ILVNSNPATIMTDPELADATYIEPVQWKEVARIIEIERPDALLPTMGGQTALNCALDL
                     VREGVLAKYSVEMIGATREAIDRAEDREKFRQLMIKIGLDMPRSAIAHSLEEAIQVQA
                     RLGFPAIIRPSFTMGGSGGGIAYNREEFEEICIRGLELSPTRELLIDESVLGWKEYEM
                     EVVRDKNDNCIIVCTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAIKVLRAVG
                     VDTGGSNVQFAINPEDGRMLVVEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPSIDYVVTKVPRFNFDKFPQTPDTLTTQMKSVGEVMAIGSNFQ
                     ESLQKAIRGLEIGRCGLHSLFQKEDMARLRGHLREPTPDRLWYIADAFRQNLSLEEIH
                     LESRVDPWFLAQIQELVLLERSIIGKTIRDLDKTTLHLMKHRGFADAYLAKLLGCTEA
                     EVRARRVELEILPVYKRIDSCAGEFPSDTAYMYSCYEGSCEARPESNKKKIMILGGGP
                     NRIGQGIEFDYCCVHAALSLRSAGYQTIMVNCNPETVSTDFDTSDRLYFEPVTLEDVL
                     SIVAMEQPEGVIVHYGGQTPLKLARDLEANGVKIIGTSSDAIDMAEDRERFQQMVKEL
                     KLHQPDNGTVRSEAEAIELANKIGYPLVVRPSYVLGGRAMEIVYQEEDLRYYLSHAVE
                     VSNDSPVLLDKFLNDAIEVDIDAVCDGKDVLIGAIMEHIEQAGIHSGDSACTLPPFSL
                     GVVVQQDLIEQIRKMALKLGVVGLINAQFAIQADDIYVLEVNPRASRTVPFVSKATGL
                     PLAKIAALCKVGISLKDQGLNQNHHAPSFYSIKLPVFPFIKFSGVDSILGPEMKSTGE
                     VMGIAKRFGQAYAKAQLGAGCHIAKRRRAFVSVRDADKTRVGEIAKRLIELGFEIIAT
                     RGTALALQAAGVDCRRVFKVAEGRPHVLDFIKNNEIDFIVNTTEGKQAIADSFAIRRN
                     ALQHKVSYTTTLSGAEAACLAMKYEDRETVTRLQDLH"
     gene            complement(31982..32254)
                     /locus_tag="ACF2SP_000714"
     CDS             complement(31982..32254)
                     /locus_tag="ACF2SP_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358519.1"
                     /translation="MNEMDILSLFYDEMIARGVTREQVFLSIEEDAAAMLTQKLSKPV
                     SVEEAQKLTDICIANEWLERTTADPYYKYLSLTEAGLQILLSNLYK"
     gene            32367..>32517
                     /locus_tag="ACF2SP_000715"
     CDS             32367..>32517
                     /locus_tag="ACF2SP_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2358520.1"
                     /translation="MQEIDVLIIGAGAAGLMCAIEASKRKRKVFVLDHANKAGKKILM
                     SGGGRC"
BASE COUNT         9884 a         7407 c         5584 g         9642 t
ORIGIN      
        1 agcctccatg aaaaaataaa acaaataaaa tagcaattgc tcttaagcca tcaatatctg
       61 agcgatagtt cacactacaa tttccataaa atgattgttg attaatacta attacatgcc
      121 cacccttgca ctttatttct gtacaaaggt ttgcaaattg ggaaattcag agttatatca
      181 caataatctt tttttagtga gaattatttt agtgtggttc attcatcaat tgttcaatgt
      241 agcgtctgca ggttaacggt ttatataaat aattaattaa atagcgagtt ccaccagttc
      301 ctgtgattat caaagtgcca taacttaaaa tactgcctaa aatggattga cgtatgtcta
      361 tcgtttcaat tttattcagg gggatatcca cagtttgcct tactatgata ccggtacgca
      421 aaataacttg cttccttttt atggtaatgg aagaaaaatg ataggttacc caggtaatca
      481 atatccagat cagtgcaaaa aatcctaaaa aaagactcac ttcgcgcaac tggatgatat
      541 aaatataaat cgccatcact aaacccaatc cgacgaaagg accaaaaaat atgatccaat
      601 gcaatctggt aaaataaatg acattacttt ctgtagtatt ttcagccatt gatcaccctt
      661 atatacctca aaaaaatatg atattgcact taacgttaat ttgctattct atacataaat
      721 caacttattt ttcgatagtc taactcttta atagatagct aacaattaag acggacgtta
      781 tgaaatcaat cagtcaatgc ctaaacaagc aactcagtac aatttgccag cattctatgc
      841 aattggaaga gttatctctt aaaatttctg catttcttcc ttcccatttc atttctaaat
      901 gtcaagtagg tagttttaat aaagggtgtt tggttttaac cacaaccaat gctgcttttg
      961 cgtccgaaat acgttattta cttcctgaat tacgggataa attgaggaaa gaagccggcc
     1021 tttatcagct gtcttcaatt aaaattacca tcattgctcc ctcctctcaa atcgaaaacc
     1081 ctgcacaaaa aaaatctctt accctgtctg aaaaagccaa ggcaaccatt attagtgaaa
     1141 gccaacactg tacttatgag ccactgaaaa aggcattact gcatttagct caaagtgaat
     1201 aactcatcaa ccatctcatt tgtgttctga attttttttc actataatgc tcaaatattc
     1261 aactaatatg ttagagcttg ctatgcctaa aataaataaa attgttaatg gaacagacct
     1321 aaccccccat tatctgagtg agccaaacaa agaattcaaa atctaccgtt ataataagga
     1381 agtttatgct gttcgttttg agaatgatga acccatggat tatgttttaa tgtggaaaag
     1441 tcacaagagt gggcatacgc agaaaagtga aatgattaaa aataccgaag aggattataa
     1501 ggagcttgga aaaggagaac aaggaacggt atatgagaaa acagaagata aggctatgaa
     1561 agtatctcga ggcagacatc caagagaatt ttatgaagaa attaacctcc atattataga
     1621 acagcaattt ttccttaaat atcatggcat tcaagagcac tttgttctag gactctggaa
     1681 tatcaaaaat gaagaaaacg tgtatttcta catgcctaaa atcaatgcga tccctattaa
     1741 caaaaaaatc gaccagccca aaattgagga atttgttctt gcgcttaaag aacttaatga
     1801 tgcagggtac tggcatcctg atttggcaaa taatccttac catattagtc cacaaaatct
     1861 gattgcaaca gaagaaatgg taaagactat tgatcttgat ggtggctttc ggtacgacaa
     1921 gggaagagtt gatgaactta gcaggaagtc tcttgtttat ggtaaagatc aatggctata
     1981 cgtctataat ttcatctatc cacccaccga tgaggaggat aacagaattg attggaaagt
     2041 ccctatagag aaatggtatg aaaataacag agacgaatct ttatcggata atccccatac
     2101 gttgcttcga ttttaccatg agggacttat agcattaccc aaaaaacttg cccatgatct
     2161 gcatgagaca atattagaag agttatccca ggataaaaaa tttaaaaaac attcgcttga
     2221 ggtaggggtg aatcgctttt ttagcccgaa aaaacaagga atctatgagg gtttaaaggg
     2281 tgatgggcta aaaaaagtga tcctgaaaga acttagagac tcgttaactg agataagaac
     2341 aatggagcaa ttggaggaaa aaaaactgga gtttttagct tccccagaaa tgcaaattct
     2401 tgctgaaggt caggataaga caacaaaagc actgaattta aaaaccagtt ctcgtaaaaa
     2461 ggtgatggta atatttaaag aagcagaaga gcgaatattg aatagcccta atgtaagcat
     2521 ttaggacgtg tcaattttaa attctgtgaa tagcatgctt ttatgtgagt tagtttcggc
     2581 ttagttcaaa taactcttga aaaattaatt aagccttttt catggcaaac ttatagcgtt
     2641 tcgacaattt ttttaagtaa agctccattg ataacttccc cataaaaatg ggccctcact
     2701 attttcttct ttcgctaaca tttcttttga ggcaattcgc ccatcatcga tattggcttc
     2761 caatcgatta aggtgcatta taatcaaatt aatacaatgt atttaaatct ttcttaaggt
     2821 gatcatgctg aaacaaattg tgctaaccgg cctagttatt atagggattg tcattacgct
     2881 aatgtactta tttcagcgcc acctcattta tttccctaat agacataccc caaagttgga
     2941 agattacaat gcatcggata tgaaagtagt ttccttacgt acaaaagata atttacattt
     3001 aaaatcctgg tataaacctg caagcaagca tcgccctaca atactctatt tgcatgggaa
     3061 tgctgggcat attggttaca gaatgccttt ggttcgtgaa tttattgacg ctggtttagg
     3121 cgtattttta ttagaatatc gtggttatgg aggcaaccca ggcaaaccta gcgagaaagg
     3181 tctttatgca gatggagaaa cagccattga atttttaatt caacatggtg tgccatcaaa
     3241 gcgcgtgatt ctatatgggg aatccatagg taccggagta gcaactcatc ttgccacaaa
     3301 atacccggtt tgtgcggtta tgcttcaatc accttttaca tcactaacca ggcttgccca
     3361 gtatcactac ccattgaact tccttaaacc ctgggatcaa tataattcac ttgcccgtat
     3421 gaaaaaaatt aatgccccca ttctggtttt gcatggaaaa ttggatcaga tagtacctta
     3481 tcaagagggc ttaaatgttt ttaatgaagc caatgaacct aaaaaaatga tttcttttga
     3541 cgacaaagaa cataatgatt tgtggagcgc tgataatttt tcgagggaaa ttattcaatt
     3601 tgctttaaac cagtgttcct agcttttttg gatagtttat tgttaaagca ttttcatcgt
     3661 caacgttatt ttgaacttaa tcatccattt ccgcaaaggc cattgaaaat ctcctttcaa
     3721 cccactcctt attgaggtac tgaaagcaat cttgcagata atcggccaat tcctcttcat
     3781 ccaagacatg ctcaaaacct acaggttcaa cgtcatgatg aaagtattcg ccgaaatact
     3841 tcattgagaa atcaatataa tcccgagtat gcaaaataaa agaatgccac atctcatcga
     3901 ggatcaataa tggtccaaaa agataggttt ttttcccccg caaccttctt tgcttattaa
     3961 gccacatcca ggctaataaa tcctcaaaga gaaatagccc ttcttgtatg gaatattctg
     4021 gatgagaata gcaaaaatga gtcactattt tttcattttt atagtgaagt aattcagaaa
     4081 gagaaaaact cattaacgtc cgcaacaagt ttttttatta cttggtttct gagtaatgaa
     4141 tctaattatt ctacaaatca tttcttcttt agccataatt gtctcttatt gttaaaatca
     4201 ttcaagttta acccaatgtt tgtaaagggc gcaactgaat gtttttattt tcatcaatca
     4261 gtatcatatg ttgtggcggc acatcttccc atccttcatc caaatcattt agcctttcag
     4321 aagagactac aacataagct ggattacctt cttcttttat ccataaccct tgagtagata
     4381 atacataccc aagcaaataa tgcagcgtca aaggtttttt cttctcatga ctgcaatagc
     4441 gggttgcgat aatcttttta ccatttgtaa gacaaatatt aaaataggaa ggtccactat
     4501 ggccaaattg tcctattact tccaatattt tgttaatagt ttcttgcagc acatcagcag
     4561 ccaccgatag ttgattaatg tctcttcctt ttgctaattg caaaaacaaa ccaaacaaat
     4621 gctcagaatc tgtttgacct ttaacccaat ggtaaatgtc atcatccaac aaatgcctta
     4681 tatgcctttt cacatcaata aaatcattaa tttccccatt atgcattaac atccaatcac
     4741 cataaataaa gggatggcag ttataattgg ttactcctcc tgcactggca gagcgaacat
     4801 gagcaaaaaa acaaggagac tgaatttttg ctgttaagtg taaaagattt ctatcgttcc
     4861 aggctggata aacggaggtg aacaaagcag gatcagagct aacttctggt gaataccatc
     4921 ccaaaccaaa accatctcca ttggttcgat atttggtttc gcgcgcatgc agactctgca
     4981 tgatgattga attcttaggt cttaccaaca cattttccaa tagcgtaggg cgccccaaat
     5041 aagcaacgaa tcgacacata acagcctccc tcatcgagtg attcgaatag caaactctaa
     5101 ttaattaact aacttccaac tttctattac tcatatccct aaatctgtaa tccattcggc
     5161 tgtcattgtg cgtttttata aggtacccgg aatcactgca aatcaactca ctttaccata
     5221 tgatgcaacg attcttactt atcatagggc taccatttgg aatattaaat gcctcttgaa
     5281 attgaggaat atttgccaag ctaccattga ctctgtatac agcaggcgga tgtggatctg
     5341 ttgtcacttg attacgaagt tgctccggcc tgacattcat agcccaaaca tgagctgttc
     5401 ctaagaaaaa ttgttgatca ggggtaattc cttctatcgt tttagcattc ttgtactctt
     5461 ttgaacgctg aaacgcttta tatgctaaaa tcattccgcc cagatctgca gttgcttcgc
     5521 caacaaccaa ttgtccctgt acatgcagat cgccatctac tacatatttt gagaattgat
     5581 tagcaatgca ttgtgtcgca tttttgaatt ttgataaatc atttggcgcc caccaatttt
     5641 tcaaatttcc ataaccatca aattgagcac cttgatcatc aaatccatgc gtcatttcat
     5701 gacctataac atatcctatt gctccataat ttatggcagt cggagccttt ggatcaaaaa
     5761 agggagactg taatatacct gcgggtatat tgaggttatt cattgaagga tcataatacg
     5821 cgttgatcgt ttgaggcgtc atcgcccatt cacttctatc tacaggttta ccaattttgt
     5881 cgagatctcg attgatcaag aattcgtttg ctctgataac attcaatacg taggggccac
     5941 gatctatttt caagctggaa taatcccacc atttggtagg gtaaccaact cgttcttcca
     6001 ttaaatccag ctttttcaat gccgcatcac gagtcttggg tgacatccag cttaaagttt
     6061 ttatatcttc ttgtagtact gctctgatat tttttagtat atccaagacc ttttgtttgg
     6121 attcaggaga aaaatatttc tctacataca attctccgat tgcaaatccc aatgcaccat
     6181 tttcagtgtt aactactctt ttccaccttg gtaatagttt ttgtgtacca gttaatgcag
     6241 aaaccatctt aaaattctga tcgacaaacg gtttggataa ataaggagcg aatgcatcta
     6301 tcaaatgcca acgcagataa attttccatt catccaagct aactgactgt aataattcat
     6361 tcatcgcttt gaaaaaatca ggcatagcaa aattgatttt tttaattttg ccctgacctc
     6421 tagcgctcaa gtattgagac caggaaaaat taggtgtcaa tttatccaat tcagtaatgg
     6481 tcatcatatg ataaatggcg tgtggatctc gttgctccac ttgtgacatg gatgctttag
     6541 ccaattgagt ttcaatatcc attacaattt tagcttcttt tgccgccatt tcaggactgt
     6601 cacccaatag ctcaaacatt ttagtaagat ggttaacata agcttctctt acctgtttaa
     6661 atttagcttc ttttttaaga taataatctc tgtctgggag tcccaatccc ccttgcataa
     6721 cagcgcctat catggaagtg ctgtccttaa aatcttgcat gctgccaaaa ttaaacaagg
     6781 gccctacgcc tattttgtgc agatgggcaa tttcagcctg taaatctttg agattcttaa
     6841 tttcttctat tttagtgaat tctggtttta gaggcgtgat acctgactta ttaatgctat
     6901 caacatccat accgctaaaa taaaaatccc ccactttctg ctcaatactt ccaggtttag
     6961 catgagtatt cttggatgca ttgattaaca tctgatgaat aatattttgt actttctcat
     7021 ttacaacata aaaacttccc caggaagcat agtcagcggg tattgggtta ttctttttcc
     7081 aattgccatt ggcataggta taaaaatcca cagctggtga cacactggta tcacgccagt
     7141 caagatgaag aagatcgttt gtattattat cggaaaaatt ggagttggaa gaaaatacta
     7201 taaaactaga taacatcgta acaatacaag ttgcaatctt aaatttcatc ctgatattcc
     7261 ccttaatttg agctttatat aaatttctaa agaaggaaac tgcttaggcc attatattta
     7321 gtatagataa tgcctataaa atgataataa tgaaaatgaa tattctcact acgtggatat
     7381 gatgtatttc tgtcaggctt caacaggata atattcaggg tgaaccgcgg gcagataatt
     7441 ttctgtatca aaataggtat attcccaagc atcctgcctt accattaatt cacgcaacaa
     7501 tctgttgttc aattcgtgac cagatttata tccttcaaat gcgccaatta aactggaacc
     7561 caataaataa agatcaccga tagcatctaa tactttatga gtaacaaatt ctgattcgaa
     7621 gcggagacca tcttcattaa ggacacgata atcatctact acaatagcat tatctagact
     7681 accgcctttt gccaagtcgc actctctcaa tttttcataa tctgaaagaa aaccaaaagt
     7741 tctcgctcta caaacttctt tgacatacga tgttccagaa aaatcaaagc ttactgtttg
     7801 tggtctatca ttaaaaaccg ggtgctcaaa acctatggta aatgttattt tatatccttt
     7861 atgaggaagg aactgaacat acttaccatt ttcttcgact cgtataggtt taagaattcg
     7921 gatatacctt tttgctgcat tttgctctct tatacccgct gattgaatta aaaatacaaa
     7981 tggggcggca cttccatcca taattggaat ctctggagca ttgacatcta tatatgcgtt
     8041 atcaattcct aacccggcca gggcggataa taaatgctcc accgttgcaa ttttcacctt
     8101 gccatgatga agggtggtgc aaagcatagt atctccgacg tattcgtagg aggcaggtat
     8161 ttcgacaact ggtgacaaat ccacgcgtct aaaaacgata cctgtgttta caggagctgg
     8221 tcttaaggtt aaaagcactt tctcaccgga atgtaatcca acaccagtag cctggattac
     8281 ctttttagga gttctttgtt ttatcattca gtgctcacct ttctctctta tatttatttt
     8341 gcacgcaaaa accttaagat cttagcaaca atttattgat tagtctaatt tttttaattt
     8401 caattaatgc ggttttggaa acccaataca aaaattgtaa aaaataacca cacgaatcaa
     8461 ttcatgagat actcaagaaa gaacgtttgt atccatcaaa tacatccttg ttttcaaaca
     8521 catcctctct caagatatcc cgttaaaaat gcttctatgc ttcttcttgc ctacgtaaaa
     8581 acgccggtat gtccaggtaa tccacatcag gaacactctc attaccatta ttggtttgct
     8641 ttgtagctgt tgttgcagta ccagtctgag cctgcttacg aatcaccgcc ggtctctcca
     8701 gttgttgata atcaagagac ccatcgcttc ttgtcgtttc aaccaggcgt gctctaagag
     8761 gttgttgtgg ctgttgctga cgctgtctat tatcacctaa accagtcaca atcacagtca
     8821 ctctcatctc gtctgtcatt tcaggatcaa taacagttcc aacaactaca gtggcatcat
     8881 cagaaatgaa ttctttaacc acgtcaccca cttcttcaaa ttcaccaata gacatatcaa
     8941 ggcctgcggt gatattcact aatattccgc gagcgccgga gaaattcaca tcctccaata
     9001 aaggagatgc aatcgctgct tcggccgctt gacgtgccct ttgctcacca actgcgcttc
     9061 ctgttcccat catggccatg cccatttctg acatcaccgt acgcacatcc gcaaaatcaa
     9121 cgttgatcaa accagggcga gtaattaaat cagaaatacc tttcacagca ccaagcaaca
     9181 cattatttgc agctttgaac gcattgagca aactgatatt ttttcctaaa acacttaata
     9241 atttattatt aggaatagta attaacgaat ccacgtgctc agccaagcgt cgaatacctt
     9301 cctcagcagc aagcgccctt tgttttcctt caaaagaaaa tggtttagtt accacagcga
     9361 ccgttaaaat acctaattcc ttagcaattt cagcaaaaac aggcgcagct cctgtccctg
     9421 ttcctccgcc cattccggct gtaataaaga ccatatctgc accactaaga atctctttaa
     9481 tgtgctctct gtcttcttct gctgcttctc ttccaatttg tggattagca cctgccccca
     9541 agcccttggt caatgcatca cccaattgaa tgtgtatttt ggcactggaa cctcttaaag
     9601 cttgtgcatc ggtattcgca caaataaact ccacaccatc aatattttca gcgaccatat
     9661 gttcgacagc gttaccaccg ccaccaccaa cacctacaac cttgatgaca gcattatttt
     9721 cttgctggct gctttccatt aattcaaaca taaccctgtc tcccctaatg tgctgctttt
     9781 ttcttattaa ataaattaaa cagttttgaa cttatcgcat ttgacacttt acaagacaaa
     9841 aatctgcatt tgttgtcatg caaaatatca tgcaacccca tatctcaatt tctatccata
     9901 ccaggaacat ttttaagcat catatcagtg tttttacata aaaagctaaa aatttccttg
     9961 aaaccattct ttcatgcgtg accaaatgct tttaccatta tcattcattg ctggcacatt
    10021 atagccactt tcatattgct gttgaaagcc atgaagcagt aaaccaaccc ctgttgcaaa
    10081 cgaagggttt tctgtcgctt cagtcaaacc ggagacataa tgcgcacatc cttttctgac
    10141 aggcatttca aaacaaagtt cggccaactc aattgctcct cttacattgg aagctccacc
    10201 tgttagaaca atgccagcag ccaaacggtc ttcaaacccg cttcggcgta attcattacg
    10261 taccagtgaa aatagttcct cgtatcgagc tgaaacgaca tctgccaaag ctttggccga
    10321 gatttttcgc ccaggcctat cattgacact ggtaacttct aacatttggt ttggattggc
    10381 taattctggt aatgcacaag catgattgac cttaattgct tctgccgcct tggttggcgt
    10441 acgcaaggcc attgcaatat catttgtcac ttgatcacct gcaatcggta tgactgaagt
    10501 atgctggata gctccctcac aaaatatcgc gatgtcggta gtgccgccac ctatgtcaat
    10561 taaacaaacc ccaagctctt tttcatcctc agtaagaaca gcatggcttg aagctaattg
    10621 ctccagaata atatcaatca catccaaacc acagcgcctg acacacttca caatattttg
    10681 ggcagcactg acagaaccag taacaatatg aacccgagat tccagcctta caccagccat
    10741 acctatcggt tcacgaatac tgccttgatg atcaatgata aattcttgcg gtaatatatg
    10801 aagtattttt tgatcagcag ggattgccac ggcctttgct gcatcgataa ccctttccac
    10861 atcggcttga gatacctcct gatcacggat ggccacaata ccatgggaat tgaggctacg
    10921 tatatggctg ccagcaatgc ctgcgtaaac agtcctcacc tcacaaccag ccattaattc
    10981 ggcttcctgt acagcacgtt gaattgaatt gactgtggtt tctatatcaa ctacaactcc
    11041 tcgctttaat ccgcgagagg gatgacggcc aatacctata atttctatcg ttccatctga
    11101 tgttacttca ccaattaatg caataatttt tgaagttcca atatccaacc ctgtgataat
    11161 atttttttct atttttttag ccattatcgt cccatttgtt gtttccactg cactgccatg
    11221 ccacgtggat aacgcaggtc cacgccagcc aactgatctg ctttttcagc aaaaacagca
    11281 gggtatgctt tacaaaaacg cagcaaccgc tcttctaact ctttctttcc caagtatatt
    11341 tttacactat gattaagcag caacacccag gattgattat ctcttaaata caagcctgaa
    11401 gcatttaaac cgtatgatga taatatctta ctcaattttt cataaacttg taagacttct
    11461 aattgctgag attgcggacc ctttaatttg ggaatatcca aatcagatgg aacagaaccc
    11521 tgattgaata actttccatc ccttgtcatt agagcatcac cccaaatcgc tacgggcttt
    11581 ttttcagtga gtttaatttt caatgtatct ggccaaactc tttctacata ggccgtatca
    11641 atccaactca ttgaattcaa ctcactttgc aatcctttta ctgataataa aaagaaactg
    11701 gcatccagat atttagctaa cacattctcc aattctttat gtgttatatg ttcatacgtc
    11761 gctgcaactt tgatcgttgt aattggaaaa cgctctgcat cagcaagata taaatagcct
    11821 aagcgacccg ctagcagcaa agcactaaga attaacaaac ccattatcgt taaataacgc
    11881 aagccgacag agttcacttc cataatttag tgttcccttc acaagtaatt tgtcaccaaa
    11941 tgttgacgac aattatgatg aaccattgcc agattaatca attcatcaag taaatcagga
    12001 taagctacac cagttgcctg ccacaatttg gggtacatac ttatggaggt aaatccaggt
    12061 aatgtattta tttcattaaa gtaaatttct tctgtcttat catttacaaa aaaatctacc
    12121 ctagccatgc ctttgcattt aagataacta aaaatattcg ctgcagcctg ttttaattgt
    12181 tcttccagac tattatttaa ttgagctgga ataatcaaat ccgtttgact gctttccaaa
    12241 tatttcgcag tataagaata ataaccgtca gggtggttta cttttatctc acccaccata
    12301 cttactcttg gtttcccaca gggaatactg ttctccaaca ctgccagctc aatttccctt
    12361 ccgacaataa attcttcaac cagaatttct tcatcatagc gtaatgcatc agcaactgct
    12421 gcgtttagct catccatatt atttgctttg tgaattccca cactggaacc tagtgagcat
    12481 ggtttaacga acagaggcca accaaactcc gaggcaactt catgacaaaa ttgttgtctt
    12541 tcagacgcat tggcatgcca tgataataat ttatatcttg ctgatttgag cccattaatg
    12601 catgcgagtc gccttgccat gtctttgtcc attccgattg cagaagacaa tacgtcacat
    12661 cctacatagg cgacaccggc taactcaagt aaaccttgta aacatccgtc ctcatataaa
    12721 gggccatgaa ccactggaaa aacaatttca gcatcaactg ccagacgtcc attaattaat
    12781 aagccttcca acggagttga tttttcagta acaacaggta atttatcaga acaggcgagc
    12841 aaatcattat aatcatgacg atgaaatcga ccgcttttat ccattgcaat gggtataata
    12901 tgatatttct cggaatccag gtgttttaga accgatgcgg cagaaaccag tgacacttca
    12961 tgctcgcctg attttccacc ataaagtaaa accaaattaa cgggtttgga cattagcgtc
    13021 tccaatgata tgtacttcgc gtatcaactc tatagatgtt tgctctcgaa ctttagtttg
    13081 caccaaatga atcaaggctt caatattagc tgctgtggca gtaccttgat gattgataat
    13141 aaaattggca tgtttttcag aaacaactgc cccaccaatg cttaccccct tgaggccaca
    13201 agattcaata agtcgcgctg caaaatctcc aggaggattt ctaaataccg acccacaatt
    13261 atattcatta gttggttggg tcttggctct atgagccaat aaatccttaa ttaattgcaa
    13321 ggatgtctct ttattacctg gtgttagctg aagcttggcg gaaataaacc attcatctcc
    13381 caagccagca acatgtcgat aagctacttc aaattcttcc ggtttacggg ttctgatttc
    13441 acctcgacga tttatcgttt gtacttcaat aactgactgc caggtttcac ctccatgaca
    13501 accggcattc attcgcagag caccccccat ggtaccgggg ataccagccc aaaactctcc
    13561 ttctgacaga ttattacgcg cagaaaaacg agccatactg gcacaggata cccctgcctc
    13621 aacccgaata cagttatcac tcaatagggt catttctttt aaacagccct gagtgagaat
    13681 cacggtaccg gaaaaaccgc catcgcgaat tagagaatta gagcctaaac ctaaccataa
    13741 taatggctca tcaaaaggca atcgacttaa aaacaaagcc aaatcatcta tatttgctgg
    13801 tttatagagc ctcgctgcag ggccaccaac tcgccaggta gtatactcgg ccaaaggctc
    13861 attgaaaagc aaagtgccct gagactctgt tacatgactg ctgtccattc ccgttatgct
    13921 catgttcatt ctcacatgtt tttcatcagg ttcactgcca tttggccgat acttccagct
    13981 ccctgcatca aaataacgtc accatcttta ataaattcat ctaaagtggc tttcaacgat
    14041 tgctcgctca ctatggtcac tcgtttatcc ctggatcgta tttcattggc cagattttca
    14101 ctggtaacgc ctggaatagc tgtttctccc gctgcataga tatccatcaa taacaactca
    14161 tccgataaac ttaaaacatc gacaaattgt ctatgcaagg actgtgttct ggtataacga
    14221 tggggttgaa agacatgaac caaacgtctc tcgggccaaa cacgtctaaa agcatcaatt
    14281 gtggataaaa tttcctgtgg atgatgtcca taatcatcca caataatcgc cgcgcctttt
    14341 tcaaactgtt tttcaccaag catttgaaag cggcgtccta ccccttggaa tttttgtaag
    14401 ccacgaacaa tggaatcgtc atcaactccc aactcggtgg caattgctat agaagctaat
    14461 gcatttaata cattgtgacg gcctggatat tggaattgta tagtcagttg tttatgtggt
    14521 gcaggtcgaa cgactacaaa ctcactcagc atgccctttt gagtccaatt aattgcccga
    14581 taatgagctt cttctttaaa cccataagtt aaggttggtc tttgaatagc cgggaggata
    14641 cgacaaattt cttcatcttc caaacaaact actgctaatc cataaaaagg caaatgatgc
    14701 aaaaattcca aaaatgttgt tcttaatttt tcaaaatcac cttcataagt atccatgtga
    14761 tcagcatcaa tattagtcac taccgccatc ataggcttca aaaacaaaaa cgaggcgtcg
    14821 ctctcatcag cctctacaac aaaataagct gactttccaa gctgagcgtt cgcaccacaa
    14881 ctattcagct ttccgccaat aacaaaactg ggatccaaac ctccttcggc aagcaaacta
    14941 ctgaccaagc tcgttgttgt cgttttgcca tgagtgccgg caattgctat accatgccta
    15001 aatcgcatta gctcagcaag catagctgct ctaggaataa ctggaatcat taactcacga
    15061 gcagccacaa tttcgggatt attaaaatca acggccgaag accttactac aacatcagcg
    15121 cctttaatgt tttccagcct gtgaccgata tagacttgaa tacctaatga ccttaagcgt
    15181 tgtaccgtgc cactttctcc gagatccgaa ccagtaatac ggtagccctg gttatgcaat
    15241 acttcggcaa taccacacat accagcacca ccaatgccaa caaaatgaat ttgctctaca
    15301 cgccccatgc gcggagataa aaattgctct gagttattca atattccccc ttaagccgtt
    15361 agcggtttca acccaagaga ctgccaacga ttttcatgat ctatgcgtaa taatagtgca
    15421 ataacgatac aattaattac catactggcc ccaccataac tgagtaaagg cagagttagc
    15481 cctttggttg gtaacagtcc ggcattaaca cccatattaa tagaagcctg taaagctaac
    15541 caaatagtca aaccataggc tgtataggat gcaaaatgcc tttcttgagt ataggcggta
    15601 tatcctatgt ttaatcctct aataaccagt atactataca atgtgatgac aactaaaatt
    15661 ccaaataacc ctaattcttc agcaatcaca gcaaataaaa aatcagtatg cgcctcaggt
    15721 aaatagagta atttctgtat gctttcacct aatccggtac caaaccatcc acctcgtcca
    15781 aaagcaatca atgactgagt caattgataa ccactattgt attgatctgc ccacggatcc
    15841 aaaaaggctg ttaagcgagc aacccgatag ggggaagata ccgccaacag agcaagtgct
    15901 gtcactacaa ccagcattaa accaaaatag tatcttaatt taactccagc taaaaataac
    15961 atagccatta ctgtaccaga aatgacaact gtcgctccaa aatctggttc cagcagcaat
    16021 aaaactgaaa caacagccag tattgccatg ggtttaatga atccaaaaat gctttcacaa
    16081 accgcctctt gctggcgaac caaatacccg gaaagataga atatcattgc aagtttcgtt
    16141 aattctgaga cttgcacacc aatcggacct aaagctaacc aacgccgact gccattcact
    16201 gatttaccaa tacccgggat taaaacaatg agcaataaaa acacgcaacc aatcatcatg
    16261 ggcatactta ttttttccca aaaactgctg tctgtacgca ccacaattaa ggccagcaag
    16321 aggcccacaa ataaataaca agcctggcga ataagaaaat gaaaaggttg atgaaagtac
    16381 tttgttgaaa tcataaccga gcttgatgcc accatcatca aaccaattat cagcaaacca
    16441 aaaactgccc ctatcagcca tttatcataa agtgatatcg gtctgctaac gggtttgcct
    16501 ctttgattta aatgtctcgg tcgcatcgtg ttcacaatcc gcgcactgaa gatgtaaaca
    16561 catcgcccct atgattaaaa tcacggaaca tatccaaact ggcacaagct ggagataaca
    16621 aaacgacatc accaggtttc gcacatgtct tggcaatagt cacagcgccc tccagagaag
    16681 aagcacgcac gacaggaact actttggcta aagccgattc aatcttgtca gcatcttcac
    16741 caatcaaaac aatggaacga acaaattctg aaactggctg ggctaattct tgaaaatccg
    16801 ccccttttcc ctgtccaccc gcaataagaa caattttccc ttgcatagaa cctcctattc
    16861 cattaatagc agagattgta gcaccaatat ttgttccttt cgaatcatta atccaaccta
    16921 ctccatccac ttctcttacc cactggcatc gatgtggcag tccagggaag gtttttaaaa
    16981 cattaagaat gtgttgcata gagattcccg ctgcttcggc taaagcacaa gcagcaagcg
    17041 cattcatcca attatgcacc ccttttatta aaatcgattc cacaggcaaa agacgctcca
    17101 ttcctttcgc caaataagta gtattctctt gttcaatcag cccccaattt cccattgaag
    17161 gtgcgtcttt gccaaaagag atgcatttta tatctgtttg acaggattgg tgaggaacgg
    17221 tatagacgtc ttctcgatta aataaaacag ccttggcacc acgataaatc ctttgttttg
    17281 cttgcgtata ggcttccata gtgtgatgcc tgtccaaatg atcaggcgtt acattaagaa
    17341 tcgtcgcaac cactggcgac aaagaataag ttaaatccaa ttgaaaactg gataactcca
    17401 atacccacaa atcgtagtga tgttcatcat ccaacatatc taagacagga gtgccgatat
    17461 ttccggctac agctacgcga aacccggccg ccttagccat ttctcccacc aaagtcgtta
    17521 cagtggattt gccattagtt cccgttatgg caatgacagg ggcgctaatc tctctggcca
    17581 ggcattctat gtctccataa attgatgcac ccgcttgacg cgctctttct aaaacaggcg
    17641 tatccagtgc caaaccgggg ctagtgataa catccgtcac ctggctgatt acttcatcag
    17701 gagtctgttg caggtaaatt ggcacatcag gaaattcatt ttggaattca gctaatccag
    17761 gagcctcttt acgtgtatca aaaacaacaa acgatttatt atttctcttc aggtatcttg
    17821 ctatagagag tcccgtctta cccagccctg ctaccaaata taaggaatga ttcatgaatg
    17881 caccttgtct atcgaagttt taaagtagcc aatccgcata acacaaatac aaccgtaata
    17941 atccaaaacc ttactatcac tttgggctct gaccacccct ttaattcaaa atggtgatgt
    18001 aatggggcca tcctgaataa tctttttccg cctgaatatt taaaataacc cacttgtaat
    18061 atgacagaaa gcgtctcaat tacaaataat ccacccataa tgagcagaac aagttcttgc
    18121 ctaactacaa tcgcaactat ccctaaagct gctcctaacg ccaaagagcc cacatcgccc
    18181 atgaaaactt gggcaggata actgttatac caaagaaaac ccaggccggc accaacaata
    18241 gaagaacaaa aaatcgttag ctcaccagta ttgggcacat aagggattcc taaataatta
    18301 gagtatacgg cgttactcga tgcataggca aacacaccca aggcaccagc caccattact
    18361 attggcataa tagccaagcc atccagccca tcagttaaat tcactgcatt actgcttcca
    18421 acaatgacga aataggcaag tacaggaaaa aaaacgccca gatcccaagt gaccgtttta
    18481 aaaaaaggaa ctgtgagctg ggtatgtact ggcaaactgg cattccaata caaataagag
    18541 acagcaacta aagcaatgac tgactgccaa aaatatttcc aacgaccagg taatcctttg
    18601 ctatttttta ataccaactt gcgataatca tcaacccaac caaccaaacc attagccaag
    18661 gtaaccaaga gcaccagcca caggcttgtt tgtcttaaat cgcaccagag caagcaactc
    18721 acagtgatag ccaataaaat caaaacgccc cccatagtgg gagttcccgc ctttgataaa
    18781 tgggtttggg gaccatcact cctaaccatt tgacctattt gcaaccctct taaccagcgg
    18841 atcattaatg ggccacataa caaacccact ataagggcag ttaatgaggc taaaatagac
    18901 ctgaatgtca aatattgaaa aactctaaac gcatgatatt gtccctgcaa taactgcgtt
    18961 aaccaataga gcataattat tcctcttgtt cttaaaatta tgctcaatga cttttattga
    19021 gcataattcc tttaatgggg caaaaaagtg gccaagcata ccacattcct gtcgattcaa
    19081 tgaagaggtt agccctctac ctgaaccaac cggtgcacaa cattttccat agcactggac
    19141 ctggatcctt ttaccaatac cgtggtatca ggtgttaaat cattttttac cacttcaatc
    19201 aactcttctt gactgcaaaa atgctggcct ccctcaccaa acgcctggga tgccagttca
    19261 ctatgtaaac cgcaactcat caacttatct attcccagct ggcgagccgc tatgccaact
    19321 tctctgtgat gttgtgtcgt ccattcaccc aactctccca tatcaccgaa tacaaaaatt
    19381 tttcttcccg ggcgcccaga taaaacttcc agggctgtta agacagagcg taaattagca
    19441 ttgtatgtat catcaataac tgttgattga ttttgccctg acaacatcgt catccgacct
    19501 tttacaccac caaaatgagc cagtcctctt tgaatgtcat tgcctgatat tcctacagca
    19561 taacaacaag ctgcggcagc caaagcattt cggacattgt gcaaacctgg tacttgtaac
    19621 tcaatctcca ctctgctatt aggtaaaacc aatgtaaaat gagctcgccc atgggtatcc
    19681 aagcgtatat cctgggcgta aatatctgca ggatgaagtg aggaaaaacg taatattttt
    19741 ttatcagcaa tcaaatcatc ccaaaagtga gcgtaggcat catcatcatt gactatagca
    19801 acaccagtcg gggataagcc ttgatggatt tcacctttgg aacgagcgac tccgtctatt
    19861 gatccgaagc cttcgacatg tgccggggca atattattaa ttaaagacac atcaggctgc
    19921 actatcccta ccgtatgcgc tatttctccg ggatgattgg ctcccaactc aaaaacagca
    19981 tagcggtgct gcttatttaa agctaacatg cttaaaggaa cccctatgtg attatttaaa
    20041 ttgcctttcg tagcgtgtga tggctgcggt aaaatagtcg ctatcatctc tttgacagtg
    20101 gtttttccat ttgaccctgt taaagcaata atagggcaac tcatttcctg tcgatgtgcc
    20161 gcagcaattt tggccaaagc cattaaagga ctgacaacca caaattgagg aatatgaacg
    20221 ccgtcaatca atcgattaac tacagcagct gaagcgcctt tagcctcggc ttctttaata
    20281 aaatcatgac catcaaagcg ttcccccact atcgcaaaaa ataaatttcc tggctttaat
    20341 gtgcgggtat ctatactaac accagtaatg tcggcattaa tctggcaaga ctgagaaaga
    20401 agtgaagcaa ttgtaattag gttcatagta ttttgtgatg tcgaaaagac tagtgtacta
    20461 acactcaggc tcacagttca agatggtttg agaatagata gataagaata taactaaaaa
    20521 gtcaagaatt ttaagtttat tacaatccga ttcataagtt atcgaacatt aaccattaac
    20581 agagtagata atgagcttcg cctggtgcga agccctatcc tgaaaatata accactagat
    20641 agtgcaatga ttctagccta ttggcattag tttgctacac aatcatccat caaatgctca
    20701 attttcaaat atcgatggta tcgtgcgata cgctcctccg ttagaggttt gcgctggtca
    20761 tccagcaaat aagcgtcgag cccctcactt aattgttttg cagtatctaa cataacagaa
    20821 aaccgttctg cattgatctc agggttattt gatatttgca tgaacaggca taatcctctt
    20881 acactgaaag caccgatatt ctgtaaatca aaaacaccag ttgctgtagc tgcagctaaa
    20941 ctgcaaagca ctggaccttg cccattcgtg aattgatgtc tatgaaacaa gtgcctctct
    21001 ccaaatctca aaccagcagc caagacagtt tgcaataatt cataaccggc aaattgtcga
    21061 ttttccttgg ccaacaaaaa caccatggcg gtggaagaag aacagtcact attaggttct
    21121 gttctttcat ttgattcgca ctcttcttcc tcaggaatca actgaggttg ttctgttttt
    21181 acagagggct tccttattgc ttgtcgcttt tcagaaccat caaaattttc aaaatccaaa
    21241 ggaacctccc tattgacctt ccgcacagcg ataatttcat cattatatga ttgagagcca
    21301 taggagttat tttctacttg gccaagagaa ggttgatagt gttcttgatt taagctctgt
    21361 cttctagtct tcattaaacg gccaatagcc actagaacgc caattaacaa caggacatta
    21421 agaattaagc tccaatttgc ctgcataagt ttactccata gtcaaagctt gtttcacatc
    21481 aaccgcaacc aaacgagaca ctcccggctc acgcatggtc accccaacta aatgttcagc
    21541 caattccata gttaccttat tatgagtaat aaacaggaat tgtatgaatt gtgacatctc
    21601 ttttactaaa tcgcaaaacc gacttacatt taagtcatct aaaggcgcat ctacttcatc
    21661 taacatacaa aatggtgaag gatttagctg aaaaattgca aacaccaaag ccacagcagt
    21721 cattgccttt tcaccaccag ataatagatg gatagaacta ttacgttttc caggcggctg
    21781 cgccattact acaatccctg cttctaacag attatcacaa gttaattcga gctgtgcccg
    21841 accaccgcca aataatcttg gaaaaagcgc tttaaaagag gtgttcactt catcaaacgt
    21901 attttcaaga cgcgatttcg tttcattatc cattttttca attgcagttt ccaaagtaga
    21961 taaagcttgg ctcaaatcat tatattgctc atccaggaac actttgcgct gctgttcggc
    22021 agcaaattct tcgatggcag ccaggtttat agcgcctagc cttttaattt tctccaaaat
    22081 cgctatcaat tcgtcttctt ttatactttg cgtgactccg gatggaattc gctctaataa
    22141 agactgggct tgtaaattcg cctcatcaag agtttcttga attgaactgg cacgaacgga
    22201 tagggcttgt ttttccattt ggatttgatt catgagttcc tgaatccgtt ttacttcctg
    22261 atcatagttt aaaattaatt tctcttggtc ttcaagttgc aacttaagct gagatacttt
    22321 ttctcggctc aaagtcaaca tcgattcgat ttcaccatgt tttatcaata atgctgccaa
    22381 ttgattttta aactcagctt ctggcgctac tgactgtaaa taatgttggg ttaattcctc
    22441 taaacgcatc tgtgcgcgtt gcaaacgctc tttttctcta cttatcctat cggttatttg
    22501 ctgtatcttg gtgacttctc tatcatattc caactcactt tgatgcaaca aagcacggac
    22561 actctctact tgtttatttt gttcaacgag aacactgttc catgtgtctt tttcatttat
    22621 aaatcgcttg aattgcaact cagaagcttg gtattgacct tccagttctc tgattttatc
    22681 agccatttcc aattgttcag cgtttagctc ttccagcgac gactccaact ctattatctc
    22741 gttcgtgata gcatctttct gtttttcagc gtgaaaaata gcttgttcct gactatttac
    22801 cgcaatgata tgattgcgta gcgcctcatt actggcattt aaatttaatt gatgcagttt
    22861 gataaaattt aaattttcct gtagccgcgc ttgataacta tctcttgcta aacggatgga
    22921 ttcaattttt tgttgtaaag attccacact taaattcaac tcgacaatcc tttgctgtct
    22981 ggcaagaaca cctacctcat cttgctcttt ttggcgaggt aatttaatcc accccttgcc
    23041 tagccaaata ccattgacgg ttacaataga ttcatgagcc aataaatcag gaaaccacgc
    23101 ctttgcctct tcaagattgt ctgcggcata aatgtgttca agatggttta ctgttgaggg
    23161 aaccaaacct tgaattttat cagctaacct tgggcgctcc tgcttttccc cattgagagc
    23221 tctcatcgtc actacgctct ctttatgatc ttcgcaaaac tgccaccgag accatagctc
    23281 agcaaaatca tccaatacat aggcgattag atcgtcccct aatacgattt cacaagaaga
    23341 ttgccattgg ggttcaacct ttaaaacatc cattaatctt ggcttatctg accattccgc
    23401 aattccgtcc ttattaggcc gattttcctt ttttgctgca atttgagcag cttttaatgc
    23461 agcaagctca ctgtttaaac gatgaaattc atcttgcaat gcatgcagtt gttgctcagt
    23521 attttgtaat tgattctgca aatccgattg agtctcattc gcttgcctaa gctgttcctc
    23581 atcaaactcc tgtgtttctt ttaattggaa atgcttttct tgcaatttaa ttttattttt
    23641 ttgcaaatcg tcaagcgaga tagctgattg ttccaaattc agtttttcca gccttaccag
    23701 gacttgatgg cgcttctgct ctaaatgttg tagtttggct tgagtaagtt ggtattcatt
    23761 tttaatggta ttggaattaa tttgcacctc ttgccaaatt ttctcccatt gcgattgttc
    23821 cttttgtttt tcttgccata gagattcctg ttctttgaat tccattttta aatgatgcaa
    23881 atgctcctct aactgttgag ccttaagctt gcaattgtgc aattcttcat tatccagctt
    23941 aagttgctct tcaatgagtt gagaatcaac ctgcatttgc tgcctgtcat tttccaaacg
    24001 ttttttttct ctttcttgtt gctgaatggc ttcttcaaga cgagctattt cagttcctga
    24061 ttgatagaga gaatcctgaa ttgattggga ttgatcaatg gcatcttgta attgttcatt
    24121 taaaactgcc cgctccttgt aacaactagt taataaactt tgttgctgct cattgcggat
    24181 tgctaactct tgtaattctc gctgttttac cgtttgtttt tctgagtatt cctgccattt
    24241 aagagcctgg atctcagctc ggcaaatttt ttcctcttcc tttaaaatgg tatagcgttc
    24301 tgctgctttg gcctgccgct ccaaacgttg caaatgtttg cccaattctt ctctaatatc
    24361 agcaacgcgc gtcaggttat cccttgtttg ttcaatacgt tgtaaagttt cccggcgacg
    24421 ctctttgtat tttgaaactc ctgccgcctc ctccaaatag gcccttaatt cctcgggttt
    24481 tgcttctatt aatcgagata cagtaccttg gccaataatt gagtaacttc gtgcccctgc
    24541 tcctgtgcct aaaaaaatat cagtaatgtc ttttcgccgg catcgagctc cattcaaata
    24601 ataaaaagac tctccatccc gagtgaccac tctctttaca gcaatttcac cataactggc
    24661 aaaaggccct gtcaatcttc ccaggctatt atcaaatacc aattctactg acgcctgccc
    24721 taccggtttg cgattggaag aaccgttaaa gataatatcc gtcatggact ctccacgaag
    24781 atttttggca gagctttcgc ccataaccca gcgaacagca tcaataatat tggacttgcc
    24841 gcatccattc gggccaacaa cagcgaccaa acgactggga aaatgaaccg tggtgggatc
    24901 aacaaatgat ttaaaaccag ccaattttaa ttgcttcaaa tgcattttaa ctgcgaatta
    24961 taaaataata atcggttagt atatcgtagg aagtgaatcc tgactatttc tgaacatcca
    25021 tatcgctccc ttctgccatc gttaaggaca acatgctcaa ttgacaaaca attttatcgc
    25081 tttataatgc atacttctgt taacaaatgt aggtaaacaa tgaaacagca attctataaa
    25141 ttgccccaaa ttctaataag cctttgtctc ctattgattc tttccacccc aatccaagca
    25201 gaaccgccaa atcttagttt aattagaaaa gaaattaaaa actattacga ctctggcctg
    25261 tattatcacg aattggaacg aaccattaag ttagctcaag aatatataca ccaacaatat
    25321 ttaatcaata aaaacaataa acacccacaa aaacttgcca tagtactgga tattgatgaa
    25381 accagcctgt ccaactatga taagatggta aaacgggatt ttaccggtag caaagagcaa
    25441 attcataaag aaatacttgc tgctaattca ccggcaatca agcctatgct gacactttac
    25501 aaaaacgctt taaaacaggg tatcaaagta ttttttgtaa ccggaaggca agaatccgag
    25561 cgcgacgcaa cccgcgctaa tttaattaaa gcaggatata ctaaatgggc gggactttat
    25621 ctacggccta atgggtattc ttccccctcg attatcccat ttaaatcaaa agcaagggaa
    25681 atgatagcta aaaagggata caccatcatt gccagcatcg gtgatcaatg cagtgatatc
    25741 caagggggat atgctaaaaa aggatttaaa ctccccaatc cattttacta cttgccttag
    25801 tcaaggtgtg atgggtgatt aggtttttcg taggtcctgt tgattttgaa tctatgaaac
    25861 ctcaggcaca acgacccgag gtttcttaat gactacaagc attaagctgc tttttctttc
    25921 atacgaattg ccatgacttc catagccagg aatttgacat aatcataggt cacgtaataa
    25981 tctccttgat ctccagcaaa tttggaccaa gaattacgca aggtgaaaac gcctttgtta
    26041 acatggcctt cttcatccat cacttcaaga tcatcatcat agccagtgat cactaattcg
    26101 tgcccagcat agatcattcc attcattgcg tccaatacaa tttcaggtgt taacatccag
    26161 gtgtcattgt aagcacggtt ggtacctaca gctccggcat ccccaacaaa cacatccaat
    26221 aacatgccga tagtcaagcg gttacctttc gccagctcct ccttgatctg atagacgatt
    26281 tgattcatat ccgctttggc tgaaaaggat tcctcatcct ttaacaaagc ctcccagcta
    26341 attaaatttg ataccgggac gctatgcgcc aggaactcgg agtcagacat gggtttacct
    26401 tcattgcttt cgtcttctaa aggatattct ctaacaccgg cgcaaccatt caatttctga
    26461 taattctgac tgatgatacc gtattcagaa atctgttgaa gaacccagta accaaaagaa
    26521 ccattccacc cgctggcttt ggctttgtca tgtatcgcca gataacttcc taactccaga
    26581 ttacacaatt gactaatgta atctcctgcc ccaagggcag catctatggc tgcagttact
    26641 gcgaaagtga cacaactacc atgatatccc tgatccaata cgggtgttaa ctgcattccc
    26701 aacttaactt ttcttggtaa ctcagaagaa aaactggctg tgtttatact atttttagga
    26761 tattctgaca gctgttcacg caaataagat ttcgcttcgg tcgataattc atacttgggc
    26821 aacttaaatg ttaaactttg agcgactcca ctgggatttt taggcgtaat gtgaacatca
    26881 acctctcctt ttaaagtcaa aggacctgca aaagccagcg atgaagagaa agacaataca
    26941 gctaaattaa ttaacttact cataaaatat atccctataa ttaaccctct tgcatcatat
    27001 caagaggtga attaaatgca aacaaatcat gcgaataaga ataaaatgac aagctagctt
    27061 tctattcaca gagtagaaac cattttaaaa taaggtacct gttcaccaga gcagtgtgat
    27121 aatttttatc aaacaaattc tatcccgaat actgaaataa catcctctca ttgacgaatc
    27181 aaaaataaca aatcagattc aatttaagaa aacaagcaac ttgacttgag ttcatatata
    27241 ataagcctga ttctaaaaaa gaggatatta atatgcgtac tctattgtca tgtttcctga
    27301 taaccctatt gtcttttggt ttaattatca atgaggcctc tgctaaacgc tttggaggcg
    27361 ggcgcagctt cggggttcaa cgctctcaaa gcagtctttt ctcatccaat aaaacacaca
    27421 atacgtcttc attaacacaa aaagctaata aaagcagatg gggtggcgta ttaggcggcc
    27481 tcctggttgg aggactattg gccagtctct ttatgggcaa tggcttggca aatgggttaa
    27541 tcacctggct aatccttggt tcagtcattt tctttgtcgt cagcttctta cgcagaaaaa
    27601 tgaatccagg ttttcaagca actcaatccg gagcattccg ccaaaactct tttgaacaat
    27661 tcacgcaaaa ctacggaaac cgaaatagtg gaggagctca ttttgcccaa caagaaacca
    27721 gctttgacgc ggacaatttt cttcgtgatg ccaaggtaac ttttatacgt ctacaagcgg
    27781 cttacgacca aaaaaatctg caggatttgc aaacattcac agtgccggaa gtatttgcag
    27841 aaattaaaat gcaactggac gaacgaggcg atgcccccaa ccaaacggaa gtcatttccc
    27901 tcgatgcaaa attgcttgat gtgtccaaac aatccttatc cacgatagcc agcgtacaat
    27961 tcactggaac aatcaaagag aacggtgaaa tatctaacct ggatgaaata tggcatttcc
    28021 gccagtttga taacagaaaa gagtgggttg tagggggtat tcagcaagaa gtatttcagc
    28081 cataaatact tatggtttcc tggagtggca actgtcttta atgacagttg cactaaaaat
    28141 caaagaaaat aactgaaatc actcaacaat atattgcacc tggatgattt catattcaac
    28201 cataccgcca ggcgtttcta ccgtgaccgc atcatcaagc tccttgccaa ttaaagctcg
    28261 ggcaatagga gaactgtatg aaatcttgtt cagtttgata tcagcttcat cctctccaac
    28321 tattttatag gttaattctg agcccgttgc tacatgacaa attgtaacgg ttgaaccaaa
    28381 tatcacttta ccattgttag gcaatttact gatatcaatt atttgcgcat gagacaactt
    28441 cgcttcaagt tcttgaatac gcccttcatt aaaactctgt tgttctcgag ctgcgtgata
    28501 ttccgcattt tctttcaaat caccatgagc tcttgcggtt gcaattgctt ctacaatgcg
    28561 cggacgatcc acgaacttta agcgatgcaa ttctgctttt agtgcttctg caccctcaac
    28621 tgtcatagga tgtttactca tatttacccc taatgtaaat cttgtaagcg agttacagtt
    28681 tccctatctt catacttcat agccaaacaa gcagcttcgg ctcctgataa ggtcgtagtg
    28741 tagctgacct tatgctgtaa ggcgttgcgt ctgatagcaa aagaatctgc tatcgcttgt
    28801 ttgccctcag tagtatttac aataaagtct atttcattat tttttataaa atccagcaca
    28861 tgtggtcggc cttctgctac tttaaagact cttctgcagt ctacgccggc tgcttgtaat
    28921 gccaaagcag taccgcgcgt ggcgataatt tcaaaaccca gttctattaa tctcttggca
    28981 atctcgccga cacgcgtttt atctgcgtca cgtacggata cgaaagctcg tcgccgtttg
    29041 gcaatgtgac atccggcgcc caattgtgct ttagcataag cttgtccaaa acgcttggca
    29101 atacccatca cttcccccgt tgatttcatt tccgggccaa gaattgaatc cacaccagaa
    29161 aacttgataa agggaaaaac cggcaactta atcgaataga aagaaggcgc atgatgattt
    29221 tggtttaatc cttgatcttt caagctaatt cctactttac aaagagccgc tattttagct
    29281 aatggcagac cggtagcctt tgaaacaaaa ggcacggttc tcgaagcgcg cggattcact
    29341 tccaaaacat aaatatcatc cgcttgaata gcaaactgcg cattaatcaa gcccaccacg
    29401 cctaatttca aagccatttt tcggatctgc tctatcaaat cctgctgaac aactacgccc
    29461 aagctaaaag gaggtaaagt gcatgccgaa tcaccagaat gtatccccgc ttgctcgata
    29521 tgctccatga tggcaccaat taatacgtct ttgccatcac aaacggcatc aatatctacc
    29581 tctatggcat cattcaaaaa tttatcaagc agaacgggtg aatcattaga aacttcaact
    29641 gcatgcgaca ggtaatacct taaatcctct tcttgataaa caatttccat agctcgtcca
    29701 ccgagaacat aagaaggcct gacaactagc ggataaccaa ttttgttagc taattcaatg
    29761 gcctctgctt cgcttcggac agttccatta tcaggctgat gcagtttcaa ttctttgacc
    29821 atttgctgaa atcgttctct gtcttcagcc atgtcaatag catctgatga agtaccaatt
    29881 atttttacgc cattagcctc taaatcacga gccaacttta atggtgtttg tcccccataa
    29941 tgcacaatga caccctccgg ttgttccatt gcgacgatgg acaacacatc ctctaaagta
    30001 acaggttcaa aatatagtcg atcggaggta tcaaaatcag tggaaaccgt ttctggattg
    30061 caattcacca taatagtttg gtaacccgca ctccttaatg acaaggccgc atgcacgcag
    30121 caataatcaa attcaatgcc ttggccaatt cgattaggtc cacctccaag aatcataatc
    30181 tttttcttat tgctctcagg acgtgcttca cagcttcctt cataacaaga atacatgtag
    30241 gctgtatcac tagggaattc tccggcacaa gaatcaattc tcttgtagac gggcaagatc
    30301 tctaactcaa cacgtcttgc ccgaacttct gcttccgtac aaccaagcaa tttggccaag
    30361 tacgcgtcag caaaaccccg gtgcttcatc aggtgtaaag tggttttatc cagatcccga
    30421 atcgtttttc caatgattga acgctctaaa agaaccaact cttgaatttg tgccagaaac
    30481 catggatcga ctcgactttc cagatgaatt tcctcaagtg ataaattttg cctaaaggca
    30541 tcagcaatat accaaagtct gtccggagtg ggttctcgta aatgtcctct taaacgcgcc
    30601 atatcctctt tttgaaacag cgaatgcaat ccacagcgtc caatctctaa acctcttatg
    30661 gctttctgta aagattcctg aaagttagaa ccaatcgcca tgacttcccc gactgatttc
    30721 atttgtgtgg taagagtgtc tggagtttgc ggaaatttat caaaattaaa ccgtggaact
    30781 ttggtaacaa cgtaatcaat gctgggttca aaggacgcag gtgttttacc tccggtgatt
    30841 tcgtttttca attcatccaa ggtataaccc acagccaatt tggctgcgac cttagcaatg
    30901 ggaaaaccgg ttgcttttga cgccaaagcc gagctgcgag atacacgcgg attcatttcc
    30961 acaaccagca tgcgcccgtc ttcaggatta atagcaaact gaacgttgga acctcccgta
    31021 tccacaccaa ctgcccttag cactttaatc gccgcatccc gcatccgttg gtattcttta
    31081 tcagttaatg tttgcgccgg agcaacggta atggaatctc cagtatgcac acccataggg
    31141 tcaaaatttt ctatagtaca aacaataatg caattatcat ttttgtcccg gacgacttcc
    31201 atttcatatt ctttccaacc cagaaccgat tcatcaatca aaagctcgcg agttggtgat
    31261 aactccaatc ctctaatgca aatttcttca aattcttcac ggttataggc tataccgcct
    31321 ccactaccac ccatggtaaa tgaaggcctg atgatggcag gaaagcctaa acgagcttgc
    31381 acttgaattg cctcttccag gctatgagca atcgctgacc ttggcatatc caatccgatt
    31441 ttaatcatca gctggcgaaa tttttctcta tcttccgccc tgtctatcgc ttcacgcgtc
    31501 gctcctatca tttcaacaga gtacttggct aatacccctt ctcttaccaa atccagggcg
    31561 cagtttaaag ctgtttgtcc tcccatcgtc ggtaaaagag cgtctggcct ctctatctcg
    31621 ataatacgag ccacttcttt ccattgaaca ggctctatat aggttgcatc agcaagctct
    31681 ggatcagtca ttatggttgc tggattagag ttaactaata tcactcgata tccttcttct
    31741 ttcaaagcgc ggactgcttg ggtacctgag taatcaaact cgcaagcctg cccaatcaca
    31801 atagggcctg ctccaagaat tagaatagat tttatatcgg ttcgttttgg cataatgaca
    31861 ataaaaaaag aataaagtga tatattttac cgagttttgc ccaaagatta tacccttcga
    31921 tgacatgttt tgagtcattt agttgcccag aaaatagcca aaggcattaa atgacataaa
    31981 attacttgta taaattagaa agcagaattt gcaaacccgc ttctgtcaaa gacagatatt
    32041 tataataggg atcagctgtt gttcgctcaa gccattcatt tgcgatgcaa atatccgtta
    32101 atttctgcgc ttcttccact gaaacaggtt tgctcaattt ctgggtaagc attgctgcgg
    32161 catcctcttc aatactcaaa aaaacctgct ctctggttac cccgcgcgcg atcatttcat
    32221 cataaaacaa ggacagaata tccatttcgt tcatagtaat tccttattat ttgatactaa
    32281 gatctatagc atatatttaa tcatcgttcg acatgataaa attaaatact ttttaattaa
    32341 aaaatttgtc ataaccaaat ctctctatgc aagaaatcga tgtacttatt attggagctg
    32401 gtgccgcagg cctcatgtgt gctattgagg caagtaagcg taaaagaaaa gtgttcgtgc
    32461 tggatcatgc caataaagcc gggaaaaaga tactcatgtc aggaggcggt cgttgta
//
LOCUS       DAXLDT010000019        32074 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXLDT010000019 DAXLDT010000000
VERSION     DAXLDT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32074
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.019"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(210..4847)
                     /gene="sdeA"
                     /locus_tag="ACF2SP_000716"
     CDS             complement(210..4847)
                     /gene="sdeA"
                     /locus_tag="ACF2SP_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SdeA"
                     /protein_id="HFD2358521.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGYGNITSGSIYNGVPTIDTVAL
                     NNQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGRTSGEGYQNILAAQLPMGS
                     TLSVIPNGSGLNMGLCGYWVASAGLRAHQALNQHNPPTLMNLGQTITDEMRNELDHDG
                     YRKITGWLLAVADEFPEGDPQLDGKALRENTEKDLKIEIPTLVLPGKDTSPKEAPVKP
                     TAPQDKSVPVWNGFSLYTDDTVKAAAQYAYDNYLGKQYTGPVESAPANFGGRMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLGKFKDGRTIADVTPEELKKIMIAQAFFV
                     AGRDDEESEKNYAKYHQQSRDAFLKYVKDNESTLIPDVFKDQEDVNFYAMVIEDKKKD
                     WDSTPAHVLINQGHMVDLVRVKQPPESYLESYFKSMQRWIGTQATEAVFATQRQFFHA
                     TYEAVTGFDSDNKEPHLVTSGQFRYVIGEDGKPIREAPKMGQKEGDIKFFPPTYKLKG
                     KERFAFGNAGLGRYVIGEDGQPIRNEPAKGEKQGTLAFFPASYQLQGNERFMRVDEYL
                     KLDEVQQRFPGIGKHLEGGMPGMNEMDYWNRLNSLNRARCENDVDFCLKQLQTAHDKA
                     KIDPIKQAFQSSKEKGRRQPNVDEIAAARIIQQILANPDCIHDDHVFINGQRLPEPFF
                     RDLLAKCDMAVVGSLLNDTDIGNIDTLMRHENDTEFHATGDEAIPKKIGEYWINDQRI
                     NNSSGSITQKKHDLIFLMQNDAWYFSRVNAIAQNRDKGSSFKEVLITTLMTPLTSKAL
                     VDTSQAPAPKRLFRGLNLSEDFTKGLIDQANAMIANTTERLFTDHSPEAFKQIKLNDL
                     SKISSRTNASTTTNINLVTETWDSNVIFEMLDPDGLLHPKQVGQHGAGTESEFSIYLP
                     EDVALVPVKVTLDGKTKTGENRYIFTFVAVKSPDFIPRHESGYAVEPFLRMQATKLAE
                     VKSSVEKAHRAPDLETIFNLQNEVEAVQYSHLSTGYKNFLKNTVSPVLENSLSGLMES
                     DPDTLSKALAAFPPDGQWSAFNFEEARQAKTQMDAIKQMVGNKIVLDALTQCQEAMEK
                     QNIAGALDALKRIPSEKEMGTIRRELREQIQSIRQDLASLQRAVITPVVTDEKKVRER
                     YDALIENTSKKITELETGKLTNLDAVKKGISNLSNLKQEVTVLRNEKIRMHVGTDKVD
                     FSDIEKLEQQIQVMDTKLADAYLLEVSKQISALENEKPKNQTELKTKIAAFIDRTADI
                     ETLRNDRIKKHGSSTDPLDLSDLDKLSGNLQRINQSLVSSLINTIRVSINQMEAKTFH
                     MQEKEIQQNFELLAKLEKTLDKSKTSEKLREDIPKLNDLLVAKQKAYPQMVQLQLKSE
                     VFVTQLREVCQANHDDLDKTRNARLRELDQLDREAGIGRMVGNLLWGFTNKVGLTTDE
                     RLDIRTKQQSLARFRTELFNDKIDTDQLISNLARKRPSELQEGLGISPDNAMDLYMVL
                     TNLESKTTSPEKLEERMKAIDDISTKIGREPEHLKFVVIDVDDTDKKAMKF"
     gene            complement(5218..10980)
                     /locus_tag="ACF2SP_000717"
     CDS             complement(5218..10980)
                     /locus_tag="ACF2SP_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HFD2358522.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHPEIKSGTIYNGVPTIDQEAL
                     DKQGFMPVLTGVGPKRDSGHWIMLIKGSGNQYHLFDPMGKNSGEGYQDILATQLPEGS
                     TLSVIPNDPGLNKGLCGYWVASVGLKARAELNKDNPPDLETLGRTTTEEMRNELTDNG
                     YLKITGWLRAVSDKFPAGDPQPDAKTLRETTEKDLHIEIPSPVPPVKDTAPKEVSTKP
                     TAPQVAPKHSLDSKLLESDDDVLDTIKYVHKEYLGKPYPGPLKNPKAPEEGRLPPNEG
                     PDRGPHGLAHTVRTMACAEVMIEEARKAQLRGETLGKAKNGQTLADVTPEELKKILIA
                     QAFFVVGRDDERSGYDEFHKRNFYAEYHEKSEQAFRKYVEDNKLIGKIFKDQKEVDFY
                     AAIILDKNHDWDATPAHILINQGHMVDLMRVKTPSEVTLEKAYNALKNTVGSKGAEAV
                     LKAHRDFFFATGAVVPLVNPEAIDDPSRGGPYENPYSGEKYVIVEGKEPKSTKDLPKP
                     VGRSYQLKDNERFLTIKEYYAFPDVQQTYPGYKTRLEASSYYFPTPFAGECEQDPAKC
                     LGAIQKARSKLQTDAIKNGFQSSPDKARRQPNMDEIAAARIIQQIMANPDCIHDDHVF
                     INGQKLPESFFRDLLAKCDMAVVGSLLNDTDVKNIDTLMRHEKDTEFHSTDPKAVPVR
                     IGDAWENRIRTKGGDVTQMKHDLIFLMQNDAWYFSRVNAIAQNRDKGSSFKEVLFTAL
                     MTPLTNKSLMDTSHVPAPKKLYRGLNLPEEFKNKLINQSNAIIANTENTLFTDLSAKT
                     FEQIKLNDFSQMSGRTCASTTTTYELTKAWDSNVVFEMLDPDGLLHPKQVGTHMAGSE
                     DEFSVYLPEDVALVPVKVTFDGKTTNGEDRCVFTLVAVKSPDFIPRHESGYAVEPFMK
                     MQKEKVNQALDAIEKDKGSYNIDEQLKNLRIEMVRQAKLPLREGIFDRLSHRLSLGTS
                     DNKISPERRDFLNQHVIPVLQECHIALRTNNMEMMQNALAKFPTDKQWSAFKSGEAVR
                     AKAQMDVLKQQIEKKIMLQTQIIPALTECGEALDKQNVTEALQALNKLPAEKEIGKAK
                     GIGQELRGQIVGVKQELTENLESMQRAVTAPVVQDAEKMRVRYETLVTDVTKRVTDFE
                     KIKPANLDSYNNAIADLNNIQQELTLLRNEKIRMHTDKDKAVDFSDIEALEKRLQDAQ
                     TKLYPTLVEEATKGIKDLEKLPEKIYFDDIKSMTSKINGYLETLEFIRNERVKKHGES
                     TEPLDMSDLDKLKNQLQGVSQHLAQILLNGAKNSLDKIKDPATLEKEGQYIKRCLDHF
                     AELEKTLDSSVKGMKQKEDFNTYKNSLMEKQEKAYPEMLQLQYKSEALITQLRNLCNI
                     HHDNLAKTREEQQQLLNKTGGILGGFFSQITTAISQGEAKKANEFARFKTELNNDKND
                     VSQLIQFLVKKKPSELEENLGISKENAEQLHGLLKQLDAKATPIDKLQENTRLIDEIS
                     TKMGSKPVSPEHVAATKAREYYQFLLYGTTQKIGNFEKVKPLDPESYKKAMSDLNNMQ
                     EALQFLRSEKSRIHDDKEKAVEFSDIEALEKRVQNAQPKLLTALLDKTTRDISALVKI
                     PRKLTFEDIKSMTTKLNSYLETLELIRNERIKQHGKSPELLDMSDLESLKGQLQTFNQ
                     NLTGMILNATKDGLDRINDRANFNEGEPYVKTCLDLLTELEKTLDSSVKGIKQKEDIS
                     ACRNSLLDKQEKANSGMLDLQSKSKDLVTQLRDICKIHHGNLAEARRTKLHSLDNQER
                     GLLGGLWSVTNKLGVTTDTVGIERMQIKMKEQALARFKTELNNDKYDTNQVIAFLANK
                     KPSELEEGLGISKENAEELHGLLSKLTSKMTSKIEIEENTQLIDEISTKIGTEPVKLE
                     LAHTVDEDESDTYRRESGYF"
     gene            complement(11260..13884)
                     /gene="sidJ"
                     /locus_tag="ACF2SP_000718"
     CDS             complement(11260..13884)
                     /gene="sidJ"
                     /locus_tag="ACF2SP_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS meta-effector polyglutamylase SidJ"
                     /protein_id="HFD2358523.1"
                     /translation="MFGFIKKVLDFFGVDQSEDNPSETAVETTDVSTKVKTTDTTQEE
                     SSVKTKTVVPTQPGGSVKPETIAPDQQRKHPIKTETTTGSTKQKSPKVTLMDGHVKQY
                     YFARRGETSTHDTSLPPPVKVLSGRSIPLKEIPFETTRNELVQIYLTSVDQLIKSNKL
                     NSIPSQQIASHYLFLRSLANSETDGIKKNQILSLAKPLGIYLASKEPHVWKTINELIE
                     KSEYPIIHYLKNNRAHSNFMLALIHEYHKEPLTKNQSAFVQKFRDSSVFLFPNPIYTA
                     WLAHSYDEDSSFNPMFRERLSTNFYHSTLTDNLLLRTEPKEVTLSSEHHYKKEKGPID
                     SSFRYQMSSDRLLRIQGRTLLFSTPQNDVVAVKVQKRGEPKSTLEEEFQMADYLLKHQ
                     SRLDVYSKLPQPLGQYSVKKSEILEISRGSLDFERFKTLIGDSKDLEVYVYKAPLTYF
                     TYLHDKNQDLEDLTASVKTNVHDLFVLLREGIMFPQLADIFHTHFGEDEREDKGRYQA
                     LVQLLNVLQFQLGRIDKWQKAVEYVNLRSSGLADLGDSLPITSLFTSSDFTKHYFSEL
                     LTGGYHPTFFDKSSGTANSLFTGKRRLFGNYLYLNTIAEYLLVIQLTLGSYGDKVTRD
                     MMDKPKKEAVWRELANVMFTSCAEAIHIMTGIPQSRALTLLKQRANIEKHFRQTQFWM
                     TPDYSKLDEDTLQMEQYSIYSGEPEYEFTDKLVSGVGLSVDGTHQDLGGYNRESPLRE
                     LEKLLYATVTLIEGTMQLDKEFFKQLQQVEKILSGEIKTDANSCFEAVAQLLDLARPR
                     CHFQKRLVLSYYEEAKLKYPSAPTDAYDSRFQVVAKTNAAITIQRFWRETRKNLSENS
                     DIESEKPESERTTDKRLI"
     gene            complement(13937..15058)
                     /gene="dupA"
                     /locus_tag="ACF2SP_000719"
                     /gene_synonym="laiE"
     CDS             complement(13937..15058)
                     /gene="dupA"
                     /locus_tag="ACF2SP_000719"
                     /gene_synonym="laiE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupA/LaiE"
                     /protein_id="HFD2358524.1"
                     /translation="MPIILDPEVLKVAEYVYQERLSKPYTEVGPEWEYNHKTPYATRA
                     TGTGHNLQRFITIDDQRLHRPIHGLAHTMRTLFYSQLMYEAAKRQPHPHRCADGRTIA
                     DLSVQDLKKLNIAQLFFVAGRESEASYGDAYHRYHLYGAKQFEAYARKHLTHLFSEKE
                     IVLYSRCIEDRIGDRFDETAEGYLIHLSHMIDLMRCKSPVEVFIGHSRGVSGIVPTLI
                     QLFGREDGLAIMHYARSLFAATGEAVPYISSSEWPHLGIESDRVERALKIVGSLEVEG
                     QEADAKKTAQAGFSVDGCYGALVKIDTPDWYHQVKEKEDYDVDEVIALPPQITIREEP
                     PKTNESFLLSLLRHVFWCCPSNQKRDDENTEVLEKKAQL"
     gene            15107..15151
                     /locus_tag="ACF2SP_000720"
                     /pseudo
     CDS             15107..15151
                     /locus_tag="ACF2SP_000720"
                     /inference="COORDINATES: protein motif:HMM:NF016529.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            complement(15272..19873)
                     /gene="sdeC"
                     /locus_tag="ACF2SP_000721"
     CDS             complement(15272..19873)
                     /gene="sdeC"
                     /locus_tag="ACF2SP_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdeC/LaiC"
                     /protein_id="HFD2358525.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHGDITSGSIYNGVPTIDTDAL
                     NRQGFMPVLTGVGPRRDSGHWIMLIKGPGNQYFLFDPLGKTSGEGYKNTLLAQLPIAS
                     TLSVIPNEPGLNMGLCGYWVASVGLKARAELNKDNPPDLETLGRTTTEEMRNELTDNG
                     YLKITGWLRAVSDKFPAGDPQPDAKTLRETTEKDLHIEIPSPVPPVKDKAPKEVSTKP
                     TAPQVAPKHSLDSKLLENDEDVLDTIKYVHKEYLGKPYPGPLKNPKAPEEGRLPPNEG
                     PDRGPHGLAHTVRTMACAEVMIEEARKAQLRGETLGKAKNGQTLADVTPEELKKILIA
                     QAFFVVGRDDERSGYDDVHKRNFYAEYHEKSEQAFRKYVEDNKLIGKIFKDQKEVDFY
                     AAIILDKNHEWDASPAHILINQGHMVDLMRTKAPAEVALERTYNTLKGTVGSKSAEVV
                     LKAHRDFFFATGAVVPLVNPEAIDDPSRGGPYENPYSGEKFVIVDDKVPASKKDLPKA
                     VNRDYKLKDNERFLTIKEYYAFPDVQQTYPGYKTRLEGSSYYFPTPFAGECEHDPAKC
                     LGAIQKARSKLQTDAIKNGFQSSSDKQRRQPNMDEIAAARIIQQIMANPDCIHDDHVL
                     INGQKLEEKFFRDLLAKCDMAVVGSLLNDTDVKNIDTLMRHEKDTEFHSTDPKAVPVK
                     IGDAWENRIRTKGGDVTQMKHDLIFLMQNDAWYFSRVNAIAQNRDKGSNFKEVLFTAL
                     MTPLTNKSLMDTSHVPAPKKLYRGLNLPQEFTNKLINQANAIIANTENTLFTDLSAEG
                     FKQIKLNDFSQMSGRTCASTTKNMKLLTDIWGSNVIFEMLDPDGLLHPKQVGTHMAGS
                     EDEFSVYLPEDVALVPTKVTLEGKTDTGEDRYIFTLVAVKSPDFIPRHDSGYAVEPFM
                     KMQKEKVTQALDAIEKDKGSYNIDEQLKNLRIEMVRQAKLPLREGIFDRLSHRLSLET
                     SDNKISPERRDFLNQHVIPVLQECHIALRTNNMEMMQKALAKFPTDKQWSAFKSGEAV
                     RAKAQMDVLKQQIEKKIILQSQIIPALTECSEALDKQNVTEALQALNKLPAEKEIGKV
                     KTIGQELRGQIVGVKQELTGNLESMQRATTAPILQDAEKMKVRYETLVTDVTKRVTDF
                     EKIKPANLDSYNKAIADLNNMQQELTLLRNEKIRMHTDKDKAVDFSDIEALEKRLQDV
                     QSKLPTQLLEQTSKDVAKLAKMPEKITFNDIKSMTSKMNNYLETLELIRNDRIKKHAG
                     STDPLDMSDLDELKGQLQTYNQSMADILLRAAKSSLDKIKDPATFEKESPYIKQCFDH
                     LAELEKTLDDSDKGRKQKEDFTAYKSALMDKQEKAYPEMLQLQYKSEALIMQLRDICK
                     IHHDNLAEARRVRLQQLDSQGGGLLGGLWTVTNTIGLTTDTVNIERMQIRMKEQTLRA
                     FKTELTNDKLNTDQVIAFLAKGSPSELQEALGISKENAEQLHGLLKQLEIKTASTDKL
                     QEVEKLIDEVSTKIGKEPVKQDHTITIDEEESHDVRYDF"
     gene            complement(20161..21957)
                     /locus_tag="ACF2SP_000722"
     CDS             complement(20161..21957)
                     /locus_tag="ACF2SP_000722"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358526.1"
                     /translation="MPKTPNNCSLSSGNKPPTFSRILFSYMAEYRWSTAFFILFGLFW
                     AFTLPYMSYLLGQIIDEIKFQNPEQVDIFSLVLTSLILYVAIHVLRSLGYYVHGLFSL
                     TSIPEKKAKLIKQLFNHLGNQSIGYFEDKHSGYLTNKITNASSSIEPLIFNLFTIIYP
                     QSLAIILTGILLSLVVPYFGVVLWLWGATLIIYSYHSAKIGNDKAMLLANSNSKVNGK
                     VVDVVNNIHTVIYNAEMSFELGNLDTDIQDMVQNDRNLQRHMTKVMLVQHIAMNILVA
                     FFLIGSVLGYNYGYVSVGDIVFVMTSVTAIAGLTSSLGKCFLDFVTNAGCLNEGLSLL
                     GDQHTITDNANAQAHQINNGEIQFHDISFAYPNQPPIFLNFNLTIPAKQKIGIVGSSG
                     AGKTTLFKLLMRLYNVEKGIIVIDDLPINAYTKKSLCSQIAIVPQHLTLFHRSIYDNI
                     TYGCNNFDKSDVILAAKKAHCHEFIAELPEQYDTIIGEQGVKLSGGQRQRIAFARAIL
                     KNAPILLLDEATSALDSRTEQAIQDGLQNLLADKTALVIAHRLSTLKAMDRILVLEDG
                     KIIENGTHTELLEKRGAYYNYWSHQSKGFIRA"
     gene            complement(22090..22956)
                     /locus_tag="ACF2SP_000723"
     CDS             complement(22090..22956)
                     /locus_tag="ACF2SP_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFD2358527.1"
                     /translation="MKNRRDEKTMLTLIIKIANDDERIKIVIMNGSRASPSAKKDIFQ
                     DYDIVYLVTEIESFVHDKNWINQFGELLIMQTPDEMDGQWPKFKGKYTYLMQFKDWNR
                     IDLTLLHIDQLETMPRDSQSLLLLDKDNLIAPFGEPSDEDYLPSPPTEKDFVNCCNEF
                     LWVSTYVAKGLWRKQLIYTKHVSEQIVKEELIKMLMWYAGIQTNFSQPMGTYGKYIEN
                     HLELELWQEFLKTYVDADYKNMWASLFKMCEIFNDLAIKISSYFGYSFNQKEFDNVVD
                     YLQDVKNNKIPP"
     gene            complement(23024..23596)
                     /locus_tag="ACF2SP_000724"
     CDS             complement(23024..23596)
                     /locus_tag="ACF2SP_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358528.1"
                     /translation="MSCYILDLIETLKNDFVKKNTPARFDSVKSYGELLEKSKINPAI
                     AAYITGTSSGVMMVDSYEKLLELSKINCAVADNMTQTQLGLDLIDSYEKFLEITKINA
                     KVAFNFQILNYQRYTKLVDSYQQSSQAKSLSGLNTSANSQLFFKKRDSNGQDRASITP
                     GQARVAHDTVGLGLEWNEAIQNTISENMMN"
     gene            complement(23830..24189)
                     /locus_tag="ACF2SP_000725"
     CDS             complement(23830..24189)
                     /locus_tag="ACF2SP_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2149 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358529.1"
                     /translation="MMSREKAAGTFFKDYQKKNVMRLLQDSLEKIINEWLKTDDESHT
                     KLKSLQELSEMDINATSFAEPSPLPDFVTRLWLDPHKALDAMDKNISKNEIRKLIKET
                     AKEIELVFTHQKAPDYS"
     gene            complement(24277..25557)
                     /locus_tag="ACF2SP_000726"
     CDS             complement(24277..25557)
                     /locus_tag="ACF2SP_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2148 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358530.1"
                     /translation="MIVRGINMTKIKLESPGFMVHKKLKSMSQSYGVMMTGVPAEVLG
                     QMQAERSIPSINKTGNLKQQIAKEVSKVCHMMTEPTQSCGQASNDVCELLLGKIEAEK
                     FHFTKYEALSADGDNLKNVLENTAPSSTNLLIRFEIDREDPPIILVKTKNENFNPETA
                     VRNKIYLLENKLYFLDKIGNLFNLGPGKKKCTQLFNAIGDSAEYSLCDPFVLEEPEKP
                     EDFAISEIVDIFNEQKERFDFWIGSHSFTIYIPQTLGESPRQFYPYQAYFGSHTLQDW
                     FVSDKDEYLSQIGIDKYIEKLAVLGKTTNTKERSDIYAEFFSKRGREAFFCAHLNEKR
                     QPLRVKFKITEINPELALKNLQETQEFIDTHPGENPSDKVENYRNRAKLAMTEHLESL
                     LDIKPESSPSLFWKNVDKKEQEMTDSSRYKKGPS"
     gene            complement(25633..27081)
                     /gene="mavC"
                     /locus_tag="ACF2SP_000727"
     CDS             complement(25633..27081)
                     /gene="mavC"
                     /locus_tag="ACF2SP_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavC"
                     /protein_id="HFD2358531.1"
                     /translation="MTTSKLEKTGLHVHEKIKHMVKSYGTMITGIPAEILGQNEAEIS
                     VGYVKKMGNMKENIAEVVRKSEMTQPTNSCGKASNEVCDLLLGTEGASEFEKSSYQVL
                     SGDGSNLKGSLPNKNLLVRVEMDRFNAPQKYQKIKREEFNPETAEKNKIYLLEDQLVY
                     LDIFGKVIDLGQTSDTCHRLFNAITTPFYQNYILYDEYIEPEESAEEAAMFEMGEIVT
                     AKMKNIDCWTATHSFTIFVPEAESEETRTLYPYQAYWTSHTLQQWFSGDKDEKLSRLG
                     IDGYIEKLALLGTTTDSKIRSSIYGELFSPPGKEHVFCTGMNEKFSPLRVKFKVTEVN
                     PEIALQNLQEVQEFIDTNYPGENAKAQCELYKTKAQEAMTQQLEMRLLTEPTHRKESV
                     TPYKEKNTQSFFSKNLDTTSRIDKMISSVITMENLKILAKEADTLSGKDREKLVEYFK
                     TLPSEQLAEIKPESETAQKAWDEQYGLVLRDK"
     gene            complement(27546..27899)
                     /locus_tag="ACF2SP_000728"
     CDS             complement(27546..27899)
                     /locus_tag="ACF2SP_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFD2358532.1"
                     /translation="MSYVDGFVAAVPKANKEKYVEHATKAAVVFKEHGALRLVECWGD
                     DVPEGKITSFPMAVQCKEDETVVFSWILWPSRQVRDAGMKKVMEDPRLKPDINPMPFD
                     GKRLIYGGFNIVVDI"
     gene            28291..30741
                     /locus_tag="ACF2SP_000729"
     CDS             28291..30741
                     /locus_tag="ACF2SP_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2358533.1"
                     /translation="MRRENTKSNIDFKSIFDSLPDLYLLLDRDFNIVGVSDAYLQATM
                     VNRKQILGKNVFDAFPENPNDPKATGAKNLYASLHRVLKNKSADTMAIQKYDIQRPAD
                     RGGGFEERYWSPINTPVLGAEQEVEYIIHRVEDVTEFVHLKKTGSAQLKLMEELRTKA
                     GEMEIEIYQRAQEIQEANKQLEEANKNLARLDQIKTQFFANVSHELRTPLMLILGSLE
                     MLLAEKRLPSFVLNTLRRIQNNAQILLKHVNDLLNISKLDAGKMILHYANIDLVKLIQ
                     QTIALFEAQIPERKFVFSMDLPDELQAQMDPDKIQHVLINILSNAIKFTPQNAKVRIR
                     LAQTNPETCRLQIEDNGSGIPPDLCETIFERFFQVEEPTTRTVGGTGLGLSIAKDFVE
                     LHGGSILAGNARNGGAIFTIELPLKAPQNVKVYSSYSQTLLGEKEISTSLAEFRNKVK
                     PIKERAEEDFEQDYYDRPLILVVEDNQEMNHFICKILNTIYRTESAFDGREGLQKSLA
                     LHPDLIVSDIMMPHMSGVKMVHAIREHPLLLKTPIIILTAKADDELTARMLEEGAQDY
                     VIKPFSAKEFKARVANLILVKKAEDELDQFVYHASHDLKAPLPAMEHLISWIEEDLGE
                     MLSEQSKKYLLLLRQRASRMSKLLDGLLKYAQSGYVFDKVRTIHTDRLITDIIKKLNP
                     PQTVTFQLEDTLPILNTCELPLREVFTALLDNSIRHHHRKDGHIIIGTKEKDRFYEFF
                     VADDGPGIEKKYQDRIFQLFQTLQPRDKLETSGVGLSIAKKIVEFQGGKISVESEKNK
                     GSVFRFLWPKDKEIGDAC"
     gene            30731..31150
                     /locus_tag="ACF2SP_000730"
     CDS             30731..31150
                     /locus_tag="ACF2SP_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2358534.1"
                     /translation="MRAKNDECTHVLLVDDDEIDLMSVQRALTKLNIPVCFHIARDGV
                     EALEQLSGNIKATKPKIIVLDIMMPKMNGIEFLRELRSHIHYNPIKVVIFTTSSNIRD
                     KQATTGLDIAGYFVKDSQFNEFIYCCKHLLEKKSHLS"
     gene            complement(31181..31698)
                     /gene="ankB"
                     /locus_tag="ACF2SP_000731"
                     /gene_synonym="legAU13"
                     /pseudo
     CDS             complement(31181..31698)
                     /gene="ankB"
                     /locus_tag="ACF2SP_000731"
                     /gene_synonym="legAU13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F-box/ankyrin repeat-containing T4SS effector
                     AnkB/LegAU13/Ceg27"
     gene            31931..>32074
                     /locus_tag="ACF2SP_000732"
     CDS             31931..>32074
                     /locus_tag="ACF2SP_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFD2358535.1"
                     /translation="MILNITAAQFPDLTLNAIESCQNIYRSIDFNFGEDADTAINKAS
                     LEKF"
BASE COUNT         8707 a         6136 c         6191 g        11040 t
ORIGIN      
        1 aaattttgaa tttacaaagt caaattgact cttttataag cgatgtagct accagggaga
       61 agaatcaagg tctaaattaa atggtatgca aactggtcga aatttcgttg ccagtagatg
      121 gatatattct cttttatttt aaatccacaa caaaaagtcg ggtcaataag acccgacaac
      181 aggaatttat gttattagat aaaagcaaat taaaatttca tagctttttt atcagtatca
      241 tcaacgtcaa tcactacaaa tttcaaatgc tcaggttctc tgcctatttt ggtggaaatg
      301 tcatcgattg ctttcattct ttcttcaagt ttttctggag aagtagtttt agactccaaa
      361 ttagttaaga ccatatataa atccatcgca ttgtcaggag atattcccaa tccttcttgc
      421 agttcagaag gtcttttcct tgccaaatta gagattagct gatccgtatc aatcttgtca
      481 ttgaataatt cagtcctgaa tcgtgcaagc gattgttgtt tagtcctgat gtcaagtcgt
      541 tcatctgtgg ttaaacctac tttgttagta aagccccata acagattgcc caccattctc
      601 cctattccag cttctcggtc cagttgatcc agttctcgta atcttgcatt tcttgtttta
      661 tccagatcgt catggttggc ttggcatacc tcacgtaatt gtgtgacgaa gacttcactc
      721 ttgagttgca gttgtaccat ttgaggatag gctttttgtt ttgcaaccag taaatcattg
      781 agtttaggaa tatcctccct taatttctct gatgtctttg acttatccaa tgttttttcg
      841 agttttgcga gtaactcgaa attctgttgt atttcctttt cttgcatgtg aaaagttttc
      901 gcttccattt gattgatgga aacccggatg gtatttatca aagacgaaac cagggattga
      961 ttgatgcgct gtaagttgcc acttaattta tccaaatcag agagatccag cggatctgtt
     1021 gatgatccgt gtttcttgat gcgatcatta cgcaaggtct caatatcagc ggttctgtca
     1081 ataaatgctg ctatttttgt tttcaattca gtctgatttt tgggtttttc attttcaagc
     1141 gctgaaattt gtttggatac ttcaagtaaa taagcatcgg ctaatttggt atccatgacc
     1201 tgtatttgtt gttccagttt ttcaatatct gaaaaatcaa ctttatcagt tccaacgtgc
     1261 atccgtattt tttcattacg taatacggtt acttcttgtt tcagattgct caagttagat
     1321 atgcctttct tgacagcatc aagattggtt aatttccctg tttcaagttc agtaattttc
     1381 ttcgatgtgt tttctatcag ggcatcatag cgttctctta ctttcttttc atctgtaact
     1441 accggggtaa taacggcacg ttgcaatgat gccaggtctt gtctgatgct ctggatttgt
     1501 tctcttagct ctcttctaat ggtacccatc tctttttcag atgggattct tttaagtgca
     1561 tccaatgcgc cagcaatgtt ttgcttttcc atggcttcct ggcattgagt caaggcgtca
     1621 agcactattt tatttccaac catttgctta atggcatcca tctgtgtttt ggcttgcctt
     1681 gcttcttcaa aattgaaggc tgaccattgt ccgtcgggtg gaaatgctgc taaggcttta
     1741 cttaaagtgt ctgggtcact ctccatgagt ccactaagac tattttccaa aacaggacta
     1801 acagtgtttt ttagaaaatt tttgtaacca gtagataaat gtgaatattg cactgcctct
     1861 acttcgtttt gtaaattaaa aattgtctca aggtcaggtg ctctatgggc tttttcaaca
     1921 gagcttttga cttcggctaa cttggtagct tgcattctta aaaacggttc aaccgcatag
     1981 ccgctttcat gtcgtggtat aaagtcaggg ctttttacgg caacaaaggt aaagatatat
     2041 cggttctctc ctgtttttgt tttcccatca agggtcactt tgactggaac tagggcaaca
     2101 tcctctggca aataaatgga gaactcactt tcagtgcctg ccccatgttg cccaacttgc
     2161 ttgggatgca gcaaaccatc cgggtcaagc atttcaaaga tgacattgga atcccaggtt
     2221 tctgttacaa gattaatatt cgtagtcgta ctcgcattag ttctgctaga tattttactt
     2281 aaatcattta acttgatttg tttaaaggct tctggtgagt gatcggtgaa tagacgttct
     2341 gtcgtattgg caatcattgc attagcttga tcgattaatc ctttggtaaa atcttctgac
     2401 aggtttaatc cacgaaacag tcttttcgga gcaggggcct gtgacgtgtc cactaatgct
     2461 ttactcgtca aaggcgtcat taaagtagta atcagcacct ccttgaaact ggagccttta
     2521 tcccggtttt gtgcgatagc attgactcgg ctaaagtacc acgcatcatt ttgcatgagg
     2581 aaaattaaat cgtgtttctt ttgggtgata ctgccactac tattattaat tctttggtca
     2641 ttaatccaat attctccaat tttcttcgga attgcttcat caccagtagc gtgaaactcg
     2701 gtatcattct catgtcgcat taaagtatca atgtttccta tgtcggtgtc attaagcaat
     2761 gagcctacaa cagccatatc gcatttggct aacaagtcac ggaagaatgg ttctggcagt
     2821 ctttggccat tgatgaacac atgatcgtca tggatgcaat caggattggc caagatttgc
     2881 tgaatgatgc gggctgctgc aatttcatcg acattaggtt gtcttcttcc tttctctttg
     2941 ctggattgaa aagcttgttt aataggatca attttcgcct tgtcatgggc tgtttgtaat
     3001 tgtttcaaac aaaaatcaac atcgttttcg catcgtgctc tgtttaaaga gtttagtcga
     3061 ttccaataat ccatttcatt catacctggc attccacctt ccagatgttt gccaatcccg
     3121 gggaatcgct gttgtacttc atccagtttg agatattcat cgacacgcat aaagcgttcg
     3181 tttccttgta actgataact agcagggaaa aaggctaagg taccttgttt ctcacctttt
     3241 gcaggttcat ttcggatagg ttggccatct tcaccgatga cataacgccc aagacctgca
     3301 ttcccgaatg caaagcgttc ttttcctttc agtttgtaag tggggggaaa aaattttata
     3361 tcaccttctt tttgccccat tttgggggct tctctaatag gcttaccgtc ttcaccgatg
     3421 acataacgga attgaccaga tgtcaccaga tgaggttctt tgttatcaga gtcaaaacct
     3481 gttactgctt cataagtggc atggaaaaat tgtctttgtg tcgcaaaaac agcttccgtt
     3541 gcctgagtac ctatccagcg ttgcatggat ttgaaataac tttccagata ggattcaggt
     3601 ggttgtttga cccgtaccaa atcgaccata tggccctgat taatcaatac atgcgctgga
     3661 gttgaatccc aatctttctt tttatcttca attaccatgg cataaaaatt gacatcttcc
     3721 tgatctttga aaacatcagg aatcagtgtg gattcattgt ctttcacata ctttaagaag
     3781 gcatcacggc tttgctgatg gtatttggca taattttttt cggattcctc atcgtctctt
     3841 ccagctacaa aaaacgcctg ggcaatcatg atttttttca gttcttcagg ggtaacatca
     3901 gcaatagtac gtccgtcttt gaatttccct aatgtttcac cccgtaattt agctttacgc
     3961 gcttcttcaa caatcaattc cgcataagcc attgtacgta aagtatggga aagaccatga
     4021 tgttgtctgt acaccattcg accgccaaaa ttcgcgggtg ctgattcaac tggtccggta
     4081 tactgttttc caagatagtt gtcataagcg tattgtgccg ctgctttaac ggtatcatcg
     4141 gtatataatg aaaacccatt ccagacaggc actgatttat cttgcggtgc agtgggtttt
     4201 acaggtgcct ctttagggga ggtatctttt cccggaagaa ccagggtggg aatttctatt
     4261 tttagatctt tttcagtgtt ttcccttaat gcttttccat ctaattgtgg gtcaccctca
     4321 gggaattcat cagcaacagc aagtaaccaa ccggtaattt ttcgataacc gtcatgatca
     4381 agttcattac gcatttcatc ggtaatggtt tggcccagat tcatcaaagt aggggggtta
     4441 tgttgattta atgcctggtg tgctcttaag ccggcagagg cgacccaata gccgcataat
     4501 cccatattca aaccagaacc attgggaata actgaaaggg tagatcccat gggtaattgg
     4561 gctgctaaaa tattttgata accttcaccc gatgttctgc ctaatgggtc aaataggtag
     4621 tattggttac caggcccttt gattaacata atccagtgtc cggagtctct gtgtggtcct
     4681 accccagtta acacaggcat aaagccctgg ttgttcaggg ctactgtgtc aatagttggc
     4741 actccattgt aaatactacc gctggtaata ttgccatacc ccatgcgtgc aaaaatggca
     4801 tgcatacctt cttgagtgag ttctacccct tcgacatact taggcattat gatttctccc
     4861 aaactgattt tcataagtga gcaaatgcgc acattaatga atataaatat ttcaaattgt
     4921 atttttatta tagaagtgta gtattaagaa agtattaaat gaaaaaaaaa tacacatatt
     4981 ataaatttga tatttaaaaa atattgggat gtgtttctct tgtatcaaga gcaaggcagt
     5041 aaaataaagg attgaaggga taagtttaag caaaaaagac gtttgccctg tttttatagt
     5101 gtttccttat tagaaatgat atgttttaaa gcagttaaca gaatgagaaa ataaaaacac
     5161 aatgtctttc tcttctcatc tgctagcatc ttcgattttt caccctcgaa caagcactta
     5221 aaagtaacca ctttctcggc gataggtatc actctcatct tcatctaccg tgtgagccaa
     5281 ttcaagtttt acaggttcag tgcctatttt agttgaaatt tcatcaatta actgtgtatt
     5341 ttcttcaatt tcgattttcg aagtcatttt actagtgagt ttacttaaca gtccatgtaa
     5401 ttcttcggca ttttctttcg atatgcctaa accttcttcc agctctgatg gttttttatt
     5461 tgctaaaaag gcaatgactt gattcgtatc gtatttgtca ttattcaatt ctgttttaaa
     5521 tcttgccagt gcttgctcct tcatttttat ttgcatcctc tcgatgccta ctgtgtctgt
     5581 ggtaacacct aatttgttag taacagacca taatccccct aataatcctc tttcctgatt
     5641 atcaagtgag tgaagcttgg tccttcttgc ttcagctaaa ttaccgtgat gaattttaca
     5701 gatatcacgt aattgggtaa ccagatcttt actcttggat tgcaaatcca gcattccgga
     5761 attggctttt tcctgtttat caaggagaga atttctacag gcgctaatgt cttctttttg
     5821 ttttattcct tttaccgaac tatccagtgt tttttcaagt tctgttaaaa gatcaagaca
     5881 tgtttttacg taaggttctc cttcattaaa gttagccctg tcattgattc tatccagacc
     5941 atctttagta gcatttagta tcatgccagt caaattttga ttaaaggttt gcaattgacc
     6001 ttttaaactt tccaaatcgg acatatccag aagctcaggg gatttaccgt gttgtttgat
     6061 tctttcatta cgaatcaatt ccagggtttc caggtaactg ttaagcttgg ttgtcattga
     6121 ttttatatct tcaaaggtca atttcctggg tatttttact aatgctgaga tgtctctggt
     6181 tgttttatcc aggagtgctg tcagtaattt aggttgagca ttttgcactc gtttttccaa
     6241 ggcttctatg tcagagaatt ccactgcttt ttctttgtca tcatgtattc gggatttttc
     6301 actacgtagg aattgtaatg cttcttgcat gttatttaag tcggacatag cctttttata
     6361 actttcagga tccaaaggtt tgaccttttc aaagttaccg attttttgtg tcgttccata
     6421 tagaagaaat tgatagtatt ctctcgcttt tgttgcggca acatgttctg gcgagacggg
     6481 tttactaccc attttggttg atatttcatc aattaatctt gtgttttcct gcaatttatc
     6541 tattggagta gcttttgcgt ctagttgctt taacagtcca tgtaattgct cagcattttc
     6601 tttcgatatg cccaaatttt cttccaattc ggatggtttt ttctttacca gaaattgaat
     6661 aagttgactc acatcatttt tgtcattatt taattcagtt ttaaatcttg caaattcatt
     6721 tgctttcttt gcctcgcctt gacttatagc agtcgttatc tggctaaaga aaccgcctaa
     6781 tattcctccg gtcttgttca gtagctgttg ttgttcctct ctcgttttgg ccaaattatc
     6841 atgatgaata ttgcatagat tacgtaattg tgtaatcaat gcttcactct tgtattgcaa
     6901 ttgcaacatt tctgggtagg ctttctcctg cttctccatt agagagtttt tataggtgtt
     6961 aaagtcttct ttttgtttca ttccttttac cgaactatcc agagtttttt caagttcagc
     7021 aaaatggtca aggcagcgtt ttatatattg tccttctttt tcgagtgtag ccggatcctt
     7081 gattttatcg agtgagtttt tagctccatt taataaaatt tgggctaaat gctgactcac
     7141 gccttgtagt tgatttttta atttatccag atcagacata tccagcggct cagtggattc
     7201 accatgcttt ttaactcttt cattacgaat gaattccaag gtttccaggt aaccattaat
     7261 tttggatgtc attgatttaa tatcgtcaaa ataaattttt tcgggcaatt tttcaagatc
     7321 tttgatccct tttgttgctt cttctacaag tgtggggtat agcttggttt gagcgtcttg
     7381 taatctcttt tccaaggctt ctatgtcaga aaaatccact gctttgtctt tatcagtatg
     7441 catgcgaatt ttttcattgc gcagaagggt taattcttgt tgtatgttat ttaaatcggc
     7501 aatggcatta ttataactgt caagattggc cggtttgatt ttttcaaagt cggtgactcg
     7561 ttttgtcacg tctgtaacaa gggtttcgta ccgtactctc attttctcag catcttgtac
     7621 aacaggggca gtgactgcgc gttgcatgga ttcaagattt tcagttagct cttgcttgac
     7681 acctacaatt tgccctctta actcttgtcc tatccccttg gctttaccta tttccttttc
     7741 tgcaggcagt ttattaagtg cttgtagcgc ttctgtgaca ttttgtttat ccagggcttc
     7801 accacattcc gtgagagccg ggataatttg agtttgcagc atgattttct tttcaatctg
     7861 ttgctttaat acatccattt gagctttggc tctgactgct tctcctgatt taaaagcaga
     7921 ccattgctta tcggtaggga attttgccaa agcattttgc atcatctcca tattgtttgt
     7981 tctaagcgcg atatgacatt cttgtaatac agggatgacg tgctggttaa gaaaatctct
     8041 gcgttcaggt gatattttgt tatcactggt tcccagagaa agacgatgag agagtctatc
     8101 aaaaatccct tctctaagag gcaacttggc ttgccgtacc atttctattc ttagattttt
     8161 tagttgttcg tcgatgttat agctgccttt atccttttca atggcatcca atgcctgatt
     8221 gactttctct ttttgcattt tcatgaaggg ctcaaccgca tagccgcttt catggcgagg
     8281 gataaagtca ggacttttta cggcaaccag tgtaaagaca caccgatctt ctccgtttgt
     8341 tgttttccca tcaaaagtga ctttgacagg gaccagagca acatcttccg gcaaataaac
     8401 ggaaaattca tcttcagacc cagccatatg ggttcctact tgttttggat gcagtaaacc
     8461 atccgggtca agcatttcaa aaacaacatt ggaatcccag gcttttgtaa gttcataagt
     8521 ggtggttgta ctggcacagg ttctgccaga catttggctg aagtcattta acttgatttg
     8581 ttcgaatgtt tttgcagaga gatcagtaaa aagcgtattt tctgtattgg caataatggc
     8641 attggattga ttgattaatt tatttttaaa ctcttctgga agatttaatc ctcgatacag
     8701 ttttttcgga gcggggacgt gggatgtatc cattaatgat ttattagtca aaggcgtcat
     8761 taaggcggta aatagcactt ctttgaaaga ggagccttta tcccggtttt gtgcgatggc
     8821 attgactcga ctaaagtacc aggcgtcatt ttgcatgagg aaaattaaat catgcttcat
     8881 ttgagtgacg tcgccacctt tggttcttat tctattttcc caagcgtctc caattcttac
     8941 cggaactgct ttggggtcgg tagagtgaaa ctcggtgtct ttctcatgtc gcattaaagt
     9001 atcaatattc ttgacgtcgg tgtcattgag cagcgagcct acaacagcca tatcacattt
     9061 cgctaacaag tcacggaaga atgattctgg cagtttttgg ccattgatga acacatgatc
     9121 gtcatgaatg caatccggat tggccatgat ttgctgaatg atgcgggcgg ccgcaatttc
     9181 atccatgttg ggttgtcttc tcgctttatc aggactggat tgaaagccat ttttaatggc
     9241 atccgtttgt agttttgagc gggctttttg gatagctccc aggcattttg cagggtcttg
     9301 ctcgcattct cctgcaaatg gtgttgggaa atagtaagag cttgcttcta aacgcgtttt
     9361 gtaaccagga taggtttgct gcacgtcagg aaaagcgtaa tattctttta tggttaggaa
     9421 tcgttcattg tctttcagtt gatagctgcg acctacaggt tttggtaagt cttttgtaga
     9481 ttttggttcc ttcccttcta caataacgta tttttctcca ctatagggat tttcataggg
     9541 cccgcctcta ctggggtcat caattgcttc agggtttact aaaggaacaa cggcaccggt
     9601 agcaaagaag aaatctcgat gggctttaag aacggcttca gctcctttcg aaccgacggt
     9661 atttttaagc gcattataag ctttctctaa tgtaacttcg ctgggagttt tgactctcat
     9721 taaatcaacc atatgtcctt gatttatcag gatatgtgcc ggggtggcat cccaatcatg
     9781 atttttgtcc agaataatcg cggcatagaa atcgacttct ttttgatctt taaagatttt
     9841 gccgatgagc ttgttgtctt caacgtattt cctgaacgcc tgttcacttt tctcatgata
     9901 ttccgcatag aaatttctct tgtggaactc atcgtaccca gagcgttcgt cgtctcttcc
     9961 taccacaaaa aaagcttgtg cgatgagaat ttttttcagt tcctctggag ttacatcagc
    10021 taaagtctga ccattttttg ctttacctaa cgtctctcct ctgagttgag ctttacgtgc
    10081 ttcttctatc atcacttcag cacaagccat tgttcttacc gtatgagcaa ggccatgagg
    10141 tcctcgatct ggcccttcat ttggaggaag tcgtccttct tccggtgctt ttggattttt
    10201 taatgggcca ggatatggtt ttcctaaata ctctttatga acatacttta tggtgtctag
    10261 aacatcatcg tcactctcta ataatttact gtctaaagaa tgcttgggag ctacttgtgg
    10321 cgcggtaggt tttgtagaaa cttccttagg agctgtgtct ttcactggag gaacaggaga
    10381 ggggatctct atgtgtaaat ctttttcggt agtttctctt aatgttttcg catcaggttg
    10441 gggatcacct gccggaaatt tatcagagac cgcccttaac cagccggtaa ttttcagata
    10501 accgttatcg gttaattcat ttctcatttc ctctgtcgtg gttcgcccca gggtttctaa
    10561 atcaggagga ttgtctttgt tcagttcagc acgagctttt agcccaacag aagcaaccca
    10621 atatccgcat agccccttgt ttaatccggg gtcattgggg ataacagaaa gggtagagcc
    10681 ctctggcaat tgagtggcta aaatatcttg gtaaccttca cctgaatttt tacccattgg
    10741 atcaaagaga tggtattgat tacctgagcc tttgattaac atgatccagt gaccggagtc
    10801 tcttttaggt cctacccctg ttaacacagg cataaaacct tgcttgtcga gagcttcttg
    10861 atcaatagta ggtactccat tataaatagt accgctttta atttcgggat gtcccatccg
    10921 agcaaaaata gcatgcatgc cttcttgagt taattctacc ccttctacat atttaggcat
    10981 tttactttct cccaaacgga tatttgtatg tacaaatact ggctctaata gacgtgaata
    11041 ttcttatgtt tattttcatt ataggattac agtattaaga aagtattaag cgcttaaaaa
    11101 atggccaaca tatcgagttg atagctggaa cagaggtggg tggctttctc ttatttttct
    11161 ataagtacaa gtctgaaaat gaagaagtct atcctgtctt atcgacttct ccatgggaat
    11221 agtgtgattt caagaagtta gaggctcaga ttgtctgttt tatatcaaac gtttatcagt
    11281 agtacgttcg gattccggtt tttcagattc gatatccgaa ttttctgaca agtttttgcg
    11341 agtttctcgc cagaatcgtt gaatggttat tgcggcattg gttttggcga ctacttgaaa
    11401 acgtgaatca taggcgtcag ttggtgcgga gggatattta agcttggctt cttcgtagta
    11461 ggataaaact aatcttttct gaaaatggca cctaggcctt gcaaggtcca gaagttgggc
    11521 aactgcttca aagcacgaat tggcatcagt ttttatctca ccagaaagta ttttttcgac
    11581 ttgctgtaat tgtttaaaaa attctttatc aagttgcatg gttccttcaa tcagtgtcac
    11641 tgtggcatag agcagttttt caagctccct taatgggctt tccctgttat atccacccaa
    11701 gtcctgatga gtgccatcga cagacaatcc aacaccagag actagtttat cagtgaattc
    11761 atattctggc tcaccggaat aaatgctgta ttgctccatt tgcagtgtat cttcatccaa
    11821 cttggaataa tcaggcgtca tccagaattg tgtctgtctg aaatgttttt caatattagc
    11881 tcgttgtttt agtagtgtca gtgctctcga ttgaggtatt ccagtcatga tatgaatggc
    11941 ttctgcacaa cttgtaaaca tgacgtttgc caattctcgc cataccgctt cttttttagg
    12001 tttatccatc atgtctcgag tcactttatc cccatagcta ccaagtgtta actgaataac
    12061 cagcaaatat tctgctatgg tattgagata caagtaatta ccaaataatc gacgcttgcc
    12121 ggtgaataaa gagttggctg taccagatga cttgtcaaag aaggttgggt gatatcctcc
    12181 tgtaagcagt tcggagaagt aatgttttgt gaaatcagat gaagtgaaca ggctagttat
    12241 gggcagactg tctcccaaat cagccagacc actgctgcgc aaattgacgt attccacagc
    12301 tttttgccat ttatcaatgc gccctaattg gaactgtaaa acattcaaaa gttgaaccaa
    12361 tgcctggtat cttcctttgt cttcgcgctc gtcttcacca aaatgagtat ggaagatatc
    12421 ggccaattgg gggaacatga ttccttcacg taatagaaca aagagatcgt gcacatttgt
    12481 tttaacagaa gctgtcagat cttccaaatc ctgattttta tcgtgtaggt aagtgaaata
    12541 agttagaggg gctttataaa catacacttc aaggtcttta gagtcgccta ttaaggtttt
    12601 aaaacgctca aaatctaatg atcccctgct gatttccaaa atctctgatt ttttaacaga
    12661 gtattgtcca agcggttgtg gtaatttgct gtatacatcc aaacgacttt gatgtttcag
    12721 caaataatct gccatttgga attcttcttc caatgtggac ttaggctctc ctcttttttg
    12781 tactttgact gcaaccacat cattttgggg tgtactaaac agtaaagttc tgccctggat
    12841 acgcaataaa cgatcacttg acatttgata acgaaaagac gaatcaattg gtcctttttc
    12901 ctttttataa tgatgctctg atgaaagagt tacttcctta ggttctgttc ttaacaagag
    12961 attatcggtc agagtggaat gataaaagtt ggtggataat ctttcacgaa acataggatt
    13021 aaagctggag tcctcatcat aggaatgagc gagccatgct gtataaattg ggttagggaa
    13081 gagaaagaca gaagagtccc tgaatttttg tacaaaggcg ctttggtttt tggttaatgg
    13141 ttccttatga tactcatgaa ttaatgccag catgaaattg gaatgagcac gattattttt
    13201 taagtaatga atgatggggt attcactttt ttcaatgagt tcattgatcg ttttccatac
    13261 atggggttct ttagaggcaa ggtagatgcc caatggcttg gctaatgaca gtatttgatt
    13321 ttttttaatt ccatcggtct ctgagttggc aagtgatcta agaaaaagat agtgagaggc
    13381 aatttgttgt gatggaatag agtttaattt gttgcttttg atgagttgat caacagaagt
    13441 gaggtaaatc tggactaatt catttctggt ggtttcaaaa ggaatttctt ttaatgggat
    13501 agaacgcccg cttaacacct tcacaggtgg tggcagagag gtgtcatgag tgcttgtttc
    13561 accccgccgg gcaaaataat attgtttcac gtgaccatcc attagtgtta ctttagggct
    13621 tttttgttta gtcgagcctg tagtcgtttc ggtttttatt gggtgttttc tttgttggtc
    13681 aggtgcgatt gtctctggtt tgacagaacc cccgggttgg gtaggaacaa ccgttttggt
    13741 tttgactgaa ctctcttcct gggtggtgtc agtggttttg acttttgttg aaacatcggt
    13801 tgtttccacc gcagtctcag atggattatc ttcagattga tcaacaccaa aaaaatcaag
    13861 tactttcttt atgaaaccaa acatactgcg tcctcatgac ctcgaaaatt ataaaataag
    13921 ctgcctcata acctcttcat agttgtgctt ttttttccaa gacctccgta ttttcatcgt
    13981 ctcttttttg atttgaagga caacaccaaa atacgtgtct aaggagtgat aacaggaaag
    14041 actcatttgt cttgggaggc tcttctctta tagtgatttg cggtggcaaa gcgatcactt
    14101 catcgacatc atagtcttct ttttctttaa cctgatgata ccaatcgggt gtgtctattt
    14161 ttaccaaagc gccatagcag ccatccaccg aaaatccagc ttgtgcggtt tttttagcat
    14221 cggcttcctg accttccact tcaagagatc ctacaatctt aagcgctctt tccaccctat
    14281 cagactcaat gcccaaatgg ggccattctg atgaactgat ataaggaaca gcctcgccag
    14341 tggcagcaaa aaggctgcgt gcataatgca taatagctaa accgtcttca cgaccaaata
    14401 gttgaatcaa ggttggtacg atacctgaaa caccgcgtga atggcctata aacacttcta
    14461 ctgggctttt acagcgcatt aaatcgatca tgtgcgataa gtgaatgaga tagccttcag
    14521 cggtttcatc aaaccgatcg cctattcgat cttcgatgca tcgagaataa aggacaattt
    14581 ccttttctga aaagagatgg gtcaaatgtt tacgggcata tgcttcgaat tgtttagcgc
    14641 catacagatg atagcggtga taagcatcac cataagaggc ctcgctttct cgtccagcca
    14701 caaaaaataa ttgtgcgatg ttaagctttt taaggtcttg cacactcaga tcagcgatag
    14761 tgcgtccgtc agcacatcga tgagggtgtg gctgtctttt ggctgcttca tacattaatt
    14821 gactgtaaaa aagagtgcgc atggtatgag ccaaaccatg aatgggccga tgaagtctct
    14881 gatcatctat cgtgatgaat cgttgcaaat tatgccctgt gcctgtagcg cgagtggcat
    14941 aaggggtttt atgattgtac tcccattccg ggcctacttc agtgtaaggt ttggataatc
    15001 tctcttgata gacatactct gctactttta atacttctgg atctaaaatt atgggcacaa
    15061 taaccacctt ttgtttaaat taaaacatag cattctttaa tgctacaaca cgaataatta
    15121 ttattcaata aggaaattta gtattcctta aaataaaaag cagcagcttg atgagaaaca
    15181 aataatatga tgttttgctt tctcatctgc tacagctttg cactattcat tacatcattt
    15241 aacaattgaa caagcactta aaaaatactc cttagaaatc atacctcacg tcatggcttt
    15301 cttcctcatc tattgtgatg gtgtgatctt gttttactgg ttctttccct attttagttg
    15361 agacttcatc aattagcttt tcaacttcct ggagtttatc ggttgatgct gttttaatct
    15421 ccagttgctt tagcaatcca tgcaattgtt ctgcattctc ttttgatatt cctaaagctt
    15481 cttgcaattc agatggactt ccttttgcta aaaaggcaat gacttgatcc gtattaagct
    15541 tgtcattagt aagttctgtc ttaaatgctc taagagtttg ctccttcatt ctaatctgca
    15601 ttctttcaat attcacagtg tctgtagtaa gacctatggt gttggttaca gtccataatc
    15661 cacctaaaag cccgcctccc tgactatcta gttgttgaag tctcactctt cttgcttcag
    15721 ctaaattgtc atggtgaatt ttacagatat cacgtaattg cataatcaat gcttcactct
    15781 tgtattgtaa ctgtagcatt tctggatagg ccttttcctg tttatccatt aacgcgcttt
    15841 tataggcagt aaagtcttct ttttgttttc ttcccttgtc tgaatcatcg agtgtttttt
    15901 caagttctgc taaatgatca aaacattgtt tgatgtatgg ggattccttt tcaaaagtag
    15961 ctggatcttt aattttgtct agagaacttt tggcagcacg caataggata tcagccatgc
    16021 tttgattata agtttgcagt tgacctttta attcatccaa atccgacata tcaagagggt
    16081 cggtagatcc agcatgcttc ttgatcctgt cattacgaat caattccaat gtttccagat
    16141 aattattcat tttggatgtc attgatttga tgtcattaaa ggtaatcttt tccggcattt
    16201 ttgctaattt tgcaacatct ttggacgttt gctctaaaag ttgagttggc aatttggatt
    16261 gaacatcttg taatcgtttt tctaatgctt ctatgtctga aaagtccact gctttgtctt
    16321 tatcagtatg catgcgaatt ttttcattgc gcagaagggt taattcttgt tgcatgttat
    16381 ttaaatcggc aatggctttg ttgtaactgt caagattggc cggtttgatt ttttcaaagt
    16441 cggtgactcg ttttgtcacg tctgtgacaa gggtttcata ccgtactttc attttttcag
    16501 catcttgtaa gattggcgca gtggttgcac gttgcatgga ttcaagattt ccagttagct
    16561 cttgcttgac accaacgatt tgccctctta actcttgtcc tattgtcttg accttgccta
    16621 tttccttttc tgcaggcagt ttattgagcg cttgtaatgc ttctgtgaca ttttgtttat
    16681 ccagcgcttc actacattcc gtgagagccg ggataatttg gctttgcagt atgattttct
    16741 tttcaatctg ttgctttaat acatccattt gagctttggc tctgactgct tctcctgatt
    16801 taaaagcaga ccattgctta tcggtaggga attttgccaa agccttctgc atcatctcca
    16861 tattgtttgt tctaagcgcg atatggcatt cctgtaatac agggatgacg tgttggttaa
    16921 gaaaatctct gcgttcaggt gatattttgt tatcactggt ttccagagaa agacgatgag
    16981 agagtctatc aaaaatccct tctctaagag gcaacttggc ttgccgtacc atttctattc
    17041 ttagattttt tagttgttcg tcgatgttat agctgccttt atccttttca atggcatcca
    17101 atgcctgagt gactttctct ttttgcattt tcatgaaggg ctcaaccgca tagccgctgt
    17161 catggcgagg gataaagtca ggacttttta cggcaaccag tgtaaagata taccgatctt
    17221 cccctgtgtc tgtttttcct tcaagagtta ccttggttgg aactaaagcg acatcttccg
    17281 gcaaataaac ggaaaattca tcttcagacc cagccatatg agttcctacc tgttttggat
    17341 gcagtaaacc atccgggtca agcatttcaa aaatgacatt ggatccccat atatcggtta
    17401 aaagtttcat atttttggtt gtactggcgc aggttctacc agacatttgg ctaaaatcat
    17461 ttaacttgat ttgtttgaag ccttcagcag agagatcagt aaaaagcgta ttttctgtat
    17521 tggcaataat ggcattggct tgattgatta atttattggt aaattcttgt ggcagattta
    17581 atccacgata cagtttttta ggagctggga cgtgggatgt atccattaat gatttattag
    17641 tcaatggcgt cattaatgcg gtaaacagca cttctttgaa attggagcct ttatcccggt
    17701 tttgtgcaat ggcattgact cgactaaagt accaggcgtc attttgcatg aggaaaatta
    17761 aatcatgctt catttgagtg acatcgccac ctttggttct tattctattt tcccaagcgt
    17821 ctccaatttt tactggaact gctttggggt cggtagagtg aaactcggtg tctttctcat
    17881 gtcgcattaa agtatcaata ttcttgacgt cggtgtcatt gagcaatgag cctacaacag
    17941 ccatatcgca tttggctaac aagtcacgga agaatttttc ttccagtttc tggccattga
    18001 tgagcacatg atcgtcatga atgcaatccg gattggccat gatttgctga atgatgcggg
    18061 cggccgcaat ttcatccata ttgggttgtc ttctctgttt atcagagctg gattgaaaac
    18121 catttttaat ggcatccgtt tgtagttttg aacgggcttt ttggatagcc cccaggcatt
    18181 ttgcagggtc gtgctcgcat tctcctgcaa atggtgttgg gaaatagtaa gagcttcctt
    18241 ccaaacgcgt tttgtaacca gggtaggttt gctgcacatc aggaaaagcg tagtattcct
    18301 ttatggttag gaatcgttcg ttatctttca gtttatagtc acgatttaca gcttttggta
    18361 agtctttctt ggaagctggt accttatcat caacaataac aaatttctcc ccgctataag
    18421 gattttcata aggtccacct ctactggggt catcaattgc ttcagggttt actaagggaa
    18481 caacagcgcc tgtagcaaag aaaaaatctc gatgggcctt gaggacgact tcagcacttt
    18541 tcgaaccgac ggtacctttc agtgtattgt aggtacgttc caatgcgact tcagcaggtg
    18601 ctttagttct cattaagtct accatatggc cttgatttat caggatatgt gccggggagg
    18661 catcccattc atgattttta tccagaataa tcgcggcata gaaatcgact tctttttgat
    18721 ctttaaagat tttgccgatg agcttgttat cttcaacgta tttcctgaac gcttgctcgc
    18781 ttttctcatg atattccgcg tagaaatttc tcttgtgtac gtcatcatag ccggaacgtt
    18841 catcatctct tcctaccaca aaaaaggctt gtgcgatgag aatttttttc agttcctctg
    18901 gagttacatc agctaaagtc tgaccatttt ttgctttacc taatgtctct cctcttagct
    18961 gagctttgcg tgcttcttct atcatcacct cagcacaagc cattgttctt accgtatgag
    19021 caaggccatg aggtcctcga tctggccctt catttggagg aagtcgtcct tcttccggtg
    19081 cttttggatt ttttaatggg ccaggatatg gttttcctaa atactcttta tgtacatatt
    19141 ttatggtgtc tagaacgtct tcgtcattct ctaataattt actgtctaaa gaatgcttgg
    19201 gagctacttg tggtgcagta ggttttgtag aaacttcctt aggagctttg tctttgactg
    19261 gaggaacagg agaggggatc tctatgtgta aatctttttc ggtagtttct cttaatgttt
    19321 tcgcatcagg ttggggatca cctgctggaa atttatcaga gacagccctt aaccagccgg
    19381 taattttcag ataaccgtta tcggttaatt catttctcat ttcctctgtc gtggttcgcc
    19441 ccagggtttc taaatcaggg ggattgtctt tgttcagttc agcacgagct tttagcccaa
    19501 cagaagcaac ccaatagcca catagcccca tgtttaggcc gggctcattg ggaataacag
    19561 aaagagtaga tgctatgggt aattgggcta ataaagtatt tttataacct tcgcctgatg
    19621 ttttacctag tgggtcaaag agaaagtatt gattaccagg ccctttgatt aacatgatcc
    19681 agtgaccgga gtctcttctg gggcctactc cggttaaaac gggcataaag ccttgcctgt
    19741 tcagggcatc tgtgtcaata gtcggtactc cattataaat gctaccgctg gtaatatcgc
    19801 catgccccat acgagcaaaa atagcatgca taccttcttg agttaattct accccttcta
    19861 cgtatttagg cattttactt tctcccaaac tgatgtttgt acatgcaaat actgattcta
    19921 atggatacga atattcacac ttggtatatt tattatagaa taatagtttt aaggaaatat
    19981 taagagttta aaatcaggct cttgattttt tatgttcgtg aaattgaaag acagttggga
    20041 ttttctgttt caaaaataag cgaggtgaat taatacgatg atataagagt ttaaagctct
    20101 aatatttata aatttttctt aggattatat taacagttta caggctggta gcagattgtt
    20161 tcatgctcgt atgaaacctt tcgattggtg tgaccagtag ttataatacg cccctctttt
    20221 ttccaataat tcggtatgtg ttccattctc gatgattttg ccatcttcta aaactagaat
    20281 tctatccatt gctttgagcg ttgatagacg gtgcgcgatc actaaagcag ttttatctgc
    20341 taacaaattt tgtaagccat cttgaattgc ctgttcagtc ctggagtcta aggctgatgt
    20401 cgcttcatca agtaacaaga taggtgcatt tttgagaata gcccgggcaa atgcaatacg
    20461 ttgtctttgt ccacctgata attttacgcc ttgctcgcca ataatggtgt catactgctc
    20521 aggcagttca gcgataaatt catggcagtg tgctttcttc gcagctaaaa tcacatctga
    20581 tttatcaaaa ttattgcaac cgtaggtgat attatcataa atgctacgat gaaaaagtgt
    20641 taaatgctgt ggcactattg caatctgaga gcaaagtgat tttttagtgt aagcatttat
    20701 tggcaagtca tctattacaa tgataccttt ctctacattg tataatcgca tcaataattt
    20761 gaataatgtg gttttaccag cccctgaact acctacaatt cctattttct gctttgctgg
    20821 aatcgttaaa ttaaaattta gaaaaattgg cggttgattg ggataagcaa atgaaatgtc
    20881 atgaaattga atctcaccgt tattaatttg atgtgcttga gcatttgcgt tatcagtaat
    20941 cgtatgttga tctcccaata agctcaaccc ttcatttaaa cacccggcat tagtaacaaa
    21001 atcgagaaag catttcccaa ggctgcttgt gaggcctgca atagcagtaa ctgatgtcat
    21061 aacaaaaaca atgtcgccta cactgacata accataatta tacccaagca ctgagcctat
    21121 taaaaagaaa gcgacgagga tattcattgc aatgtgctgt accagcatga cttttgtcat
    21181 gtggcgctgc aaattgcgat cattttgaac catatcctga atgtctgtat ccagattacc
    21241 aagttcaaaa gacatttcag cattgtagat tactgtgtgg atgttattga ccacatcgac
    21301 taccttaccg ttgactttac tattggaatt cgcaaggagc attgctttat cattaccaat
    21361 tttggcggaa tggtatgaat aaataataag tgtagctccc cagagccaga gaaccactcc
    21421 aaaatatgga acgactaaag acaataatat acctgttagg ataattgcta atgattgtgg
    21481 gtaaataatg gtaaataaat taaaaataag tggctcaata ctagaactgg cattggtaat
    21541 tttgtttgtt aaataaccgg agtgcttatc ttcaaaataa ccgatcgatt gattgcccag
    21601 gtgattaaat agttgtttaa tcagtttggc ttttttttcg ggaatactgg taagtgaaaa
    21661 caaaccatga acataatatc ccaagcttcg caaaacatga attgcaacat aaagaattaa
    21721 tgaggttagg actaagctaa agatgtcaac ctgttcagga ttttgaaatt ttatttcatc
    21781 tatgatttgc ccaagtaagt aggacatata aggtagagta aatgcccaaa aaaggccaaa
    21841 taaaataaaa aatgctgtac tccagcggta ttcagccata tatgagaaca agattcttga
    21901 gaaggttggt ggtttattac cgctggataa actgcaattg tttggagttt tgggcataac
    21961 atttccttca aactttaaaa tttgttccac ctcatatggc ggagtgctct caattcattt
    22021 gcaaaaatcg ataaccaaca tagatatctc aatagtattt attctttaat tccaagggga
    22081 tttgttcgat taaggtggaa ttttattgtt ttttacatct tgcaaataat ctaccacatt
    22141 atcgaattct ttctgattaa atgagtaacc aaaataactt gaaattttta tcgctaggtc
    22201 attgaatatt tcacacattt tgaataatga agcccacatg ttcttataat cagcatcaac
    22261 gtaggtttta aggaattctt gccagagctc aagctcaagg tggttttcaa tatattttcc
    22321 ataagttccc atcggttgac taaaattggt ttgaattcct gcataccaca tgagcatttt
    22381 aattaactct tctttaacta tctgctctga aacgtgtttt gtgtagatga gttgttttct
    22441 ccagagtcct ttggcaacgt aagtgcttac ccataagaat tcattacaac agttaacaaa
    22501 atctttttct gtaggggggc ttggtaagta atcttcatca ctaggctcgc caaaaggtgc
    22561 aattaaattg tctttatcaa gcaataagag actttgactg tctctaggca tagtttccag
    22621 ttgatcgatg tgtaacaagg ttaaatcaat cctgttccaa tctttaaatt gcatcagata
    22681 agtatattta cctttaaatt ttggccattg cccatccatt tcatcaggtg tttgcatgat
    22741 aagtaattca ccaaactgat taatccaatt tttatcatga acaaatgatt ctatttcagt
    22801 taccagatac acaatatcat aatcctgaaa aatatctttt ttcgctgaag ggcttgctcg
    22861 agagccattc ataatcacaa ttttaatcct ctcatcgtca ttagctattt tgatgatgag
    22921 tgtaagcatt gttttttcat cacgtcgatt tttcatattt ctggattatg gcctttttaa
    22981 atttaaattc tagaatagct attaggcttt gctgttaaag tttctagttc atcatgttct
    23041 cgctaatggt attctggata gcttcgttcc attccagccc caatccaact gtatcatgag
    23101 ctactctcgc ttgtcctggg gtaatagaag ctcggtcttg tccattagaa tcgcgtttct
    23161 taaaaaaaag ttgggaattt gccgaggtat ttagacctga aagagatttt gcttgtgaag
    23221 attgttgata tgagtcgacc agtttggtat acctttgata atttaatatt tgaaaattaa
    23281 aagcaacttt agcatttatc tttgttattt ccagaaattt ttcatagcta tcaattaaat
    23341 ccagacccaa ttgagtttgt gtcatattat cagcaacagc acaattaatt ttagagagtt
    23401 ctaatagttt ttcataggaa tcaaccatca taaccccaga tgaagtgcct gtgatatacg
    23461 ctgcaatcgc gggattaatt tttgattttt ccaataactc tccataagac ttgacgctgt
    23521 caaatcttgc aggggtattt ttttttacaa aatcattttt taatgtttcg attaaatcca
    23581 aaatatagca tgacattatc ttttaacccc ttatatttaa agtaatttgt ggtggaaaag
    23641 aacgaacctc tggaggcccg gttccataga cttaatttaa cataacaaat taattagaag
    23701 ggtttttaga tatgagtcaa ttttcgaatt gtttttctgt gtatgttttt atccaataag
    23761 acatcatttg gccaatgata tggccaaaat gaatgaagaa ttaatatacc agcaccatcc
    23821 tcatttcaat taagagtagt cgggtgcttt ttgatgagta aagacaagtt ctatttcctt
    23881 tgccgtttct ttaatcagct ttctgatttc gttttttgaa atgtttttgt ccatggcatc
    23941 aagcgcttta tgtggatcca gccagagacg cgtaacaaaa tcaggaagtg gtgagggttc
    24001 agcaaatgag gtagcattaa tatccatctc tgaaagttct tgaagacttt tcagtttggt
    24061 atggctttca tcatcagttt tcagccattc gttgataatt ttttcgagag agtcttgtaa
    24121 caatctcatt acattttttt tctggtaatc tttaaaaaaa gttccagctg ctttttcgcg
    24181 actcatcata aatcaaactc cttcgttatt ttcaaacacc cattaataca attttagaac
    24241 aatttataat ttaattgatt tttattgttt catttactag cttggtccct ttttatacct
    24301 gctactgtca gtcatttctt gttctttctt atcgacattt ttccaaaata aagaggggct
    24361 cgattcaggc tttatgtcta ataggctctc caggtgttcg gtcattgcca atttcgctct
    24421 atttctataa ttttctactt tatctgatgg attttctcct ggatgggtat caataaattc
    24481 ttgcgtttcc tgtaaattct ttaatgccaa ttcagggtta atttcagtta ttttgaactt
    24541 tactcgtaat ggttgtcttt tttcattaag gtgggcgcaa aaaaatgctt cccggcctcg
    24601 ctttgaaaaa aattctgcat aaatatccga gcgttctttc gtgtttgttg tttttcccag
    24661 tacagctaat ttttcaatat acttgtcgat gccaatttgc gacaggtatt catctttatc
    24721 gctgacaaac caatcctgta aagtgtgtga accaaagtaa gcttgataag gatagaattg
    24781 gcgtggtgat tcacccagcg tttgagggat atatattgta aaagaatgcg acccgatcca
    24841 aaaatcgaat ctctcttttt gttcgttaaa gatatcgacg atctcactta ttgcaaaatc
    24901 ttcaggtttt tcaggctctt caagaacaaa aggatcacaa aggctatatt ctgctgagtc
    24961 tccaattgcg ttgaataact gcgtacattt ttttttacct ggtccaaggt taaaaagatt
    25021 acctatcttg tcaagaaaat ataatttatt ttctaatagg taaattttat tcctcacagc
    25081 ggtttccgga ttgaaattct cgttttttgt tttgaccaat ataatgggag ggtcttctct
    25141 gtcaatttca aatcgtatta ataggttagt gctggatgga gctgtatttt ccaaaacatt
    25201 ttttagattg tctccatctg ctgataatgc ttcatatttg gtgaaatgga atttttccgc
    25261 ttcaattttc cctaaaagca attcacacac atcattactg gcttggccac aactctgagt
    25321 gggttcagtc atcatatgac atacctttga aacctccttt gcaatttgtt gctttaggtt
    25381 ccctgtttta ttaatgctgg ggatagatcg ctctgcctgc atttgtccta acacctcagc
    25441 agggacacca gtcatcatga ctccatagct ttgggacatg cttttcaact ttttgtgtac
    25501 catgaaaccc ggcgattcca gttttatttt tgtcatatta atgccacgta caatcaaagt
    25561 gatatattat aggtcaatat catttgatta taaacagttc aagtgacaaa tacacaaagc
    25621 cgattcgatt tatcacttat cacgaagaac taatccgtat tgttcatccc aggctttttg
    25681 agctgtttct gattcaggct tgatttctgc cagctgctct gatggtaatg ttttaaaata
    25741 ctccactaac ttttcacgat cctttccaga gagggtatct gcttcttttg caagaatctt
    25801 gaggttttcc atcgtaatga ctgatgagat cattttatct attctgctgg ttgtatctaa
    25861 gtttttgcta aagaaagatt gtgtgttttt ttctttatag ggggttactg attcttttcg
    25921 atgagtcggc tctgttagaa gtcgcatctc aagttgttga gtcatcgctt cttgcgcttt
    25981 tgttttataa agttcgcatt gagctttcgc gttttcgcca ggataattcg tatcaataaa
    26041 ctcttgaact tcttgtaaat tttgcaacgc tatttctgga ttgacttcag tgactttaaa
    26101 tttgactcgt aatggcgaga atttttcgtt catcccggtg caaaaaacat gttcttttcc
    26161 tggaggagaa aatagttctc cataaatgct ggagcgtatt ttgctatcgg ttgttgtgcc
    26221 taacaaagcc agtttttcga tatagccatc aatacccaag cgtgataatt tttcatcttt
    26281 atcaccggaa aaccactgtt gcaaagtatg gctggtccaa taagcttgat aaggatacaa
    26341 tgtgcgtgtc tcctcactct ctgcttcagg aacaaatata gtaaaagaat gggttgctgt
    26401 ccaacaatct atgtttttca ttttagctgt aactatctca cccatttcaa acatggctgc
    26461 ttcctcagcg ctttcttccg gctcaatata ctcatcataa agaatataat tttgataaaa
    26521 aggggtggta atagcattaa atagtctatg acatgtgtct gatgtctgtc caaggtcgat
    26581 gacttttcca aatatatcga gatatacaag ctgatcttcc agtagataaa ttttattttt
    26641 ttccgcggtc tcgggattaa actcctctct ttttattttt tggtattttt gaggagcatt
    26701 aaatcgatcc atttcaactc tgaccagtaa atttttattg ggtagagaac ctttcaaatt
    26761 actgccatct ccagataaga cttgatagct tgatttttcg aattcgctcg ctccttctgt
    26821 tcctaaaagt aaatcacaca cttcattgct cgcttttccg cagctgtttg tgggttgagt
    26881 catttcagat tttcgaacta cttcagcaat gttttctttc atatttccca ttttttttac
    26941 atatccaacc gaaatttctg cctcattttg ccctaatatt tcggctggaa taccggttat
    27001 catcgttcca taactcttga ccatatgttt gattttttca tgaacatgca aacccgtctt
    27061 ttcaagcttg gaagttgtca tattaatctc actgatagat accctatatt taattgtaga
    27121 ttaaaataaa ctttgtacag tttggttttt atacattaac caagagagag atgtattagg
    27181 ataatgaaat aggagagcca cttcagaaag gtcatatatt caaaatcaat tagcagatta
    27241 atagaacttt attcgaattt atttttaaaa ataaaatctt tatcctgaaa acttgcttat
    27301 gttttactaa acaataggat tttagtatta attcaaacat tcatgctggc gatgatgaat
    27361 ttggtcggtc aaataccgaa caataccagc gatactttga cgtggcagac tgtcaagaaa
    27421 atgctagagg gctttgagtg ttgacagaag aaaggcttta ccctctacca tgaattcaag
    27481 cagtataaat taatatgagt cctgagttat catttcgtct caggtgtatt tgatctttgt
    27541 attttctaga tatcaaccac tatgttgaat cctccataaa tcaaacgttt gccatcaaaa
    27601 ggcatggggt taatgtcagg ttttaaacga ggatcttcca tgactttttt cattcctgcg
    27661 tcacgaactt gcctggaggg ccaaaggatc caggaaaaaa caaccgtttc atcttctttg
    27721 cactggacag ccataggaaa agaagtgatt ttgccttcag gtacatcatc tccccaacat
    27781 tcaaccaatc gcaaagcacc atgctctttg aataccacgg cagcttttgt tgcatgctca
    27841 acgtattttt ctttattagc ctttgggact gcagcgacaa atccatcaac ataactcata
    27901 aaaacctcca tttattttat gaaacaacag attataacta tggcattgtg tgttccattt
    27961 ttctaatatt tggttgacga taagatcata aatcgataga aaaagtactg aaaaaaatcc
    28021 ttgtgaaagt agcattaagt caaatcccta aagtaagtgt tgtttttgct gaaagagatt
    28081 catcttttta ttggtgattg cagacaacta tcgaaatcag gattttctga ttagtaagga
    28141 ctcaaatcct ctattataat tagtagtagg tttaattggc tattcatgcc ttatgttgaa
    28201 aattgaacat tggattcatg gccagcctcg caaagacttg cgtcatgctt tatagccaat
    28261 aggccttaca tagaggaagg aggatgctct gtgcgaaggg aaaatacgaa atcaaatatt
    28321 gattttaaat ccatatttga ttccctgccg gatttatacc ttttattgga cagggatttt
    28381 aatatcgtcg gagtcagtga tgcctactta caggcaacaa tggtgaatcg caaacaaatt
    28441 ttgggaaaaa atgtcttcga tgcttttcct gaaaatccaa acgatccaaa ggcaacaggt
    28501 gctaagaacc tatatgcatc gcttcacagg gttctaaaaa acaaatcagc agatacaatg
    28561 gctatacaaa aatatgatat tcagaggcct gcagaccgag gagggggatt tgaagaacgt
    28621 tattggagcc ctattaatac tccggtttta ggggctgaac aggaagtgga atatattatt
    28681 catcgtgtgg aggatgttac tgaatttgtc catttaaaaa aaaccggttc agcgcagctg
    28741 aagctgatgg aggaactgcg gaccaaggct ggggaaatgg agattgaaat ctatcagcgt
    28801 gcccaggaaa ttcaggaagc aaacaagcag ttggaagagg ctaataaaaa tctggctaga
    28861 cttgatcaaa tcaaaaccca atttttcgct aatgttagtc atgaacttcg taccccatta
    28921 atgctgatac taggttctct ggaaatgttg cttgctgaaa aaaggcttcc ctcatttgtt
    28981 ctgaacacat taaggcgcat acaaaataat gctcaaattt tactgaaaca tgtcaatgat
    29041 ttgctgaata tatcaaagct ggatgctggc aaaatgattc ttcattatgc caatattgat
    29101 ttggtaaaat taattcaaca gacaattgca ttgtttgagg ctcagattcc cgaacgcaag
    29161 tttgtttttt caatggattt gcctgatgaa ttacaggcac aaatggatcc tgacaaaatc
    29221 cagcatgttt taatcaatat tttgtcaaat gccataaaat tcacaccgca aaatgcgaaa
    29281 gtaaggatta gattagcgca aaccaatcct gaaacatgca ggcttcagat agaagataat
    29341 ggctcgggta ttccccccga tctttgtgaa actatattcg agcgtttttt tcaggttgag
    29401 gaacctacaa ctcgtacggt agggggcact gggttgggtt tatcgattgc aaaagatttc
    29461 gttgaattac atgggggctc aattctggca gggaatgctc gaaatggtgg tgccattttt
    29521 accattgaat taccactaaa agcgcctcaa aatgtaaagg tttattcttc ctatagccag
    29581 acattattgg gggaaaaaga aataagcacg agtcttgctg aattcagaaa taaagtaaaa
    29641 ccaattaagg agagagctga ggaggatttt gaacaggatt attacgatag accgctgatc
    29701 ctggtagtag aagacaatca ggagatgaat cattttattt gtaaaatatt gaacactatt
    29761 tatcgtactg aaagtgcttt tgatggtcga gaggggttgc aaaaatcgct cgctttacat
    29821 ccagatctca tcgttagcga tattatgatg cctcatatga gcggtgtcaa aatggtgcat
    29881 gctattagag aacaccccct gctattgaaa actccaatta ttattttaac tgccaaagca
    29941 gacgatgaat taactgcacg tatgctggaa gaaggggctc aggattatgt gataaaacca
    30001 ttctcggcca aggaatttaa agccagagtt gctaatttga ttttggtgaa gaaagctgaa
    30061 gatgagctgg atcaatttgt ctatcatgca tcacatgatt taaaagcgcc gctgcctgct
    30121 atggagcatt tgatctcatg gattgaagaa gatctgggtg aaatgctttc tgaacaatct
    30181 aaaaaatatt tactgctctt gcgtcaacgt gcttcacgaa tgtcaaagtt gctcgatgga
    30241 ttgcttaaat acgctcaatc aggatatgtt tttgataagg ttcgtacaat tcacaccgat
    30301 agattgatta ctgatatcat aaagaaattg aatcctccgc agactgtcac gtttcaactc
    30361 gaagatacac taccaatatt gaatacttgt gagcttcctt tgagagaggt ctttacagcg
    30421 cttctcgata acagcatcag gcatcatcac cgcaaagacg gacatatcat cattggtaca
    30481 aaagaaaagg atcggtttta tgagtttttt gtagcggatg atggccctgg aatagaaaaa
    30541 aaatatcaag acagaatttt tcaattattt cagaccttac aacccaggga taaacttgaa
    30601 acaagtggtg taggtctaag cattgctaag aaaatagtcg agtttcaagg aggtaaaatt
    30661 tctgtggagt cagaaaaaaa caaaggcagc gtctttcgtt ttttatggcc aaaagataag
    30721 gagattggcg atgcgtgcta agaatgatga gtgtacccat gttttattgg tcgatgatga
    30781 tgaaatcgat ttaatgtctg ttcaaagagc gctgacaaaa ttgaatattc cggtttgttt
    30841 tcatatagct cgtgatggtg ttgaagctct cgaacaactc tctggaaata tcaaagcgac
    30901 caaacctaaa atcatcgtcc ttgatatcat gatgccaaaa atgaatggta tagaatttct
    30961 cagagaattg cgttctcata ttcattataa cccgattaag gttgttatct tcacgacatc
    31021 aagtaatatc cgggataagc aggcaacaac aggtttggat atagcaggat attttgtaaa
    31081 agactctcaa tttaatgagt ttatttattg ctgcaaacac ttgctggaaa aaaaatcaca
    31141 tctatcatag gccagacccg gtttttttaa tttaaaagct ttaacaaaca aagcacttgc
    31201 tctgtgctat tttttcttct tgcattcccc taatcaaggc gcgtatttgt ttttttcctt
    31261 atccttgaag gctggatttg ccagttgctt tttaagaaga gagataggtg aatcaccata
    31321 gttatcttta aaatttggat cgaaagtgta ttttctccag aggtaatttg cagtttgata
    31381 tgcttttcct tcgatagctg caagtaaagg tgttctattt aagtggtagc tattggtctg
    31441 ggctggatcg attccttttt ttagaatctt tttgacctct tccaggttat tgcttcttgc
    31501 cgccatcata agatttcccc atagctctcg ctttatacgt tgttgtctta gttgatgcag
    31561 ttccagatgt ttatcattag ccaagcgatt aaaaaattga catgtctgag ctatacgacc
    31621 tagtgtgttt gcttttaaga aactcaatgt attgacaatt gtttcctcag gaagatcaga
    31681 aaaaaaattc tttttcatag cagcaaatag tgatggttaa aagtttcata attttacatt
    31741 atgattatta attgaatatt aagacattgc ttcacttttt tgttaaccag gatttaatac
    31801 aagcagaaca gaatcagaat aggcaaatac aagatgcaat tgtattttta tatatcacaa
    31861 agaattattt atatgcaaaa caacctataa tcaagttagt gtcagttttg ggatgtgaag
    31921 acaggttatt atgattttga atattacagc agcgcaattc ccggatttaa cgcttaatgc
    31981 tattgaatcc tgtcaaaata tctatcgaag tattgatttt aattttgggg aagatgcgga
    32041 tacggcaatt aacaaagcca gtttggagaa attt
//
LOCUS       DAXLDT010000020        31704 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXLDT010000020 DAXLDT010000000
VERSION     DAXLDT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 31704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31704
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.020"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..705)
                     /locus_tag="ACF2SP_000733"
     CDS             complement(<1..705)
                     /locus_tag="ACF2SP_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFD2358536.1"
                     /translation="MLLKCIEKIIDGYFAVIPRHTPEFENINGALIIAHRGAHNNARG
                     IIENTLEAFQNAKEAGCWGIELDVHLTKDKVFVVNHDPTLNRLWNKDIAIADLNFSEL
                     RALECRIPSLSEVVRQYGNSMHLFIELKTDVEEEEILVQTLQHLTPCKDFHLLTLQES
                     TFKKLCSFPRQSLLLVAVHNNVKKFCELSIKERYGGVLGNYLLLTNRELKYLQDANQH
                     FGVGFVDSKNSLYRELN"
     gene            957..2627
                     /locus_tag="ACF2SP_000734"
     CDS             957..2627
                     /locus_tag="ACF2SP_000734"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HFD2358537.1"
                     /translation="MATKVTKRGMDLLRDPILNKGTAFSLQERDEFALHGLIPTTVET
                     LEQQVIRCLDAYSAKEEPLEKHIYLRALQDRNEVLFYRFIIDNLVDILPIIYTPVVGQ
                     ACEMFSHIYRQPRGVFLSYPERDKLDSIIQNIASTRSIKVIVVTDGERILGLGDQGAG
                     GLGIPIGKLSLYTSCGGIHPSNTLPIILDVGTNNKERLDDPEYIGWRHARISGKDYDD
                     FVDQFVQSIKRHMPHVLLQFEDFAQQHAYPLLERYKDQLCTFNDDIQGTASVAVAAIL
                     AATRVTNTPLKEHRVALLGAGSAGCGISEQLVHAMMNQGLSEEEARSRFYLVDRYGLL
                     HDEMTDLLPFQKGFVRSSTSLQNWKLEKQGEITLTDVINNAQPTILLGVSGQPNQFKE
                     AMIKTMLSYCERPIIFPLSNPTSRAEAIPQDLLNWTAGKALIATGSPFEPVAINGHKI
                     EIAQCNNSYIFPGVGLGVVAGQAKRVTDLMMMAAAVALSELAPAIRTGEGRLLPELNS
                     IREVSQHIARAVILQGIKEGHIGPMNDNEIDDSIKRTMWTPQYEPYVL"
     gene            2857..3825
                     /locus_tag="ACF2SP_000735"
     CDS             2857..3825
                     /locus_tag="ACF2SP_000735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFD2358538.1"
                     /translation="MSIITTNLNRAIHDLQQGKPVAIPTETVYGLAAPINNNAAIRAV
                     FEMKSRPLNHPLIVHIASNWDLHDLVEEVPEYAHQLADKFWPGPLTLVFHCKKDQINP
                     LITGGQSTIAIRCPDHPLTQKLLKKLNIPVVAPSANPFGKISPTTALHVKQSFNDAEL
                     TILDGGRCRVGIESTIVDATNPDSYQILRHGIIDEKTIAETIAIGCSTQKNDIRVPGK
                     LDSHYQPQKTLYYFDQYETLSEFCQKRKGTVYVIANRQPPGVETNLFHPLGMHPEKSA
                     YDLYYQLRLADTSDAICIAIELPPNTSEWQGVRERILKAGFPYSQQ"
     gene            3908..3994
                     /locus_tag="ACF2SP_000736"
     tRNA            3908..3994
                     /locus_tag="ACF2SP_000736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3942..3944,aa:Leu,seq:cag)
     gene            complement(3999..4850)
                     /gene="asd"
                     /locus_tag="ACF2SP_000737"
     CDS             complement(3999..4850)
                     /gene="asd"
                     /locus_tag="ACF2SP_000737"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842959.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFD2358539.1"
                     /translation="MFRDVLKTLPQYLIPKHGITALAGYFADVRNPRLKNFLIRNFIR
                     KFDVDMSEALIEDPKSYDCFNDFFIRHLKPECRPLSQSDVICPVDGCISEIGKIERGQ
                     LLQAKGKYYSVQELLACDGQLAEQFVQGQFATLYLSPKDYHRVHMPIDAELVSMTYIP
                     GALFSVQPATTRVVPKLFARNERLAIFFKTKIGPMVMVMVGATIVGAIGTSWHGDVKR
                     SKKLERFDYSEQFSDKIISQGSEMGYFKLGSTVVLLFANGEKIQWDKELLAGSKVQLG
                     KPMAIIT"
     gene            complement(4943..7558)
                     /locus_tag="ACF2SP_000738"
     CDS             complement(4943..7558)
                     /locus_tag="ACF2SP_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2975 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358540.1"
                     /translation="MGLPKKALKESQLQFLTAGTAVSDSSHQTYKVSFIENGVIKNAF
                     YKKLDPKNHYPELLAKISVAVSLFKRIFQGKRSAEERLVFDDEERLVGTLSISVDGFK
                     GFNFHKESVPQESSAKEQVIPSTRTLIEKSFMEILLGRWFLDDDDGHPHNLSLAGDID
                     FDMFFYWFTIYMKEPRPAIGIPKTRVNLTVRDWEGFPNVKDSKPFHWPTYKNPGQETL
                     PTVLPVQDKLVNLILEKTYPDPGQFEQLAHEPVAQEQKFAAALKILLTYQPEMIRKRL
                     TELFGEMTLNYTSLDETDVALRSQYEKTFPHLCNENTNIKPFVDFIMNLYQMHYDNLY
                     RVVVFYMGCENNGYGVPLPATNSALYHKPSFYKDIVEWARTQNITIFSKDDSSIKFDE
                     DELRRRYHQVWRDAYAPTFRDLLHDSYSLTNKLLQQVSTFHVVLDEVEGKKPTDDTLT
                     NAWELFGTIPELSLEKITPLISVDKDSKLRTALILLVEFTTQFHAVAKTYYQKDRKDL
                     TEEDNLEFSEQLVQLYTNYNLKIRQSLAHTSTLAGEFNRIAVGLKQYTERANFQLHLT
                     TTDEQMKEATVATTPKEILPHTHEDVIRQFNDSLFLWAKNLRPEDLSHHISEIIDKYY
                     APTIELLSKRHRAQPVKEYLQASANESGENRLAYILSAGEGDTGALNTLLIQHLTPYM
                     LQTYPLLSIRNAVKEGNFDKDLEIFTKAAVDFAKHDRRFIHLYNLEGKSLFFKTMYEW
                     IDELPATKFKGLLESALKDYEGKLWWSTSRRSEVEGYCTKFSQAKIVAMTFLNGKDSS
                     SLNDVLFDKIIAAIQKDINKNKEKLKVPGFRLINCYNAKEHRADYFKEVKNYAEPVSH
                     RQETTLNSNITSLVV"
     gene            complement(7821..9350)
                     /locus_tag="ACF2SP_000739"
     CDS             complement(7821..9350)
                     /locus_tag="ACF2SP_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358541.1"
                     /translation="MSITAQELVKQYKLRLTPAMENDLLSEESRLKKELEAVPFNSEE
                     TLYKSILQMIIVFYEENTLEENRYLLQDHELIKQLSALMWDDIQIKLIPFLIQKNFTL
                     SEIKELLFDEAYYRSLHVLVDFGLTQDIPELLAHQEKREQLKFINTLANDHCRKLCLI
                     FWVKGSLSIKEIQDIVNATSHYPMLAETLIALDKTKTISIKQLKKLALDPKKHQQESI
                     LYHYSEQFKAYNLRKSDLSQLNLDDLDALGKSFKVLKEAGIANDYAYRLVLKNNKTGQ
                     LLRLFLPGLAKIESLSHRKALIELLYIGAQKGVVTQGKALLQIKDSNLLVLARALRER
                     FICVQQMQDLGFKKEIIAFTGEENNINSSRFRHVIMRVEEKCKDIHERLRKSSLDKDK
                     VGNWQRADEKYRQTLYSIAYDGITKSGVDLHIKMKSAEKEILSIVDPEIKSIIHKVLV
                     VIANIIITALTLGFANDLKESATGNYWFFNQSPSGEVIRALNKEVLTTIDSPELITIS
                     P"
     gene            complement(9804..11465)
                     /locus_tag="ACF2SP_000740"
     CDS             complement(9804..11465)
                     /locus_tag="ACF2SP_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HFD2358542.1"
                     /translation="MKKRIVSLFVISTAISSVNAATQAIVWGESTKVLPQFMQAHPFK
                     KQGLLQTVKSQQSDYKLELQNNSNHATRHARYQITYKGVPVWGYQVIFHSKDGTKETV
                     TGMNITGIEQDINSTEGKLSPNDIEQKILGKVAEPVKFKNLKKVIYIDKGNKAHLAYH
                     LSYYSNSEKKHVNAPNYLIDANSGEILKQWDEVRHERIGQGLGGNAFTLPYRQGMFQH
                     GNALPGLPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPITIFAESVLNLSA
                     FSYPCNETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKIYGVNRPIGDDLP
                     IRAYTHLGDMDNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTA
                     LHSGLMYEGQSGGINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRY
                     LDDPAKDGMSIGHASEYTDALDVHITSGVFNKAFYLLAHKPGWSIQKAFQVMVDANMN
                     YWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS"
     gene            11717..12571
                     /locus_tag="ACF2SP_000741"
     CDS             11717..12571
                     /locus_tag="ACF2SP_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFD2358543.1"
                     /translation="MNFNIIGAGRLGKNLGIAMSVHQIASLNSICNSNLASARIACNE
                     IGFGNAVGKISDLSEADITWITCRDDSILDVVSILDNIEILKKGSLVIHCSGALNSSV
                     LLPLKKQGCYIASVHPLKAFRTNSLDSNAFKQVHCVIEGDYEACRWLRFAFEKLEASI
                     IAIQPEMKVIYHTAATIASNYLVTLASYSEELLLKAGIQQAQSRKMICDLMYSNILNL
                     NQVDSAKSALTGPLTRGDMQTISLHLEAIDNSEIKRLYKSMALATLPLLDLSLDRKET
                     LRKTLEKE"
     gene            complement(12678..14933)
                     /gene="parC"
                     /locus_tag="ACF2SP_000742"
     CDS             complement(12678..14933)
                     /gene="parC"
                     /locus_tag="ACF2SP_000742"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFD2358544.1"
                     /translation="MKKIPNMNEVTEKKPLTEFTEKAYLDYSMYVILDRALPSIADGL
                     KPVQRRIVYAMSELGLKATAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYR
                     YPFVDGQGNWGSPDDPKSFAAMRYTEARLSLYADVLLGELLQGTVDWVDNFDGTLQEP
                     ALLPARLPNILLNGATGIAVGMASDILPHNLTEVANACIHLLDNPQADIKTICEYIKG
                     PDFPTEAEIISPSETIRAMYESGNGSIKMRAIYAQEKQNIVITALPYQVSGARVMEQI
                     ALQMQQKKLPMVEDLRDESDHEHPTRLVIIPRSNRVDIESLMSHLFATTDLERSYRVN
                     FNMIGLDGKPRVKGLLTILTEWLEFRLTTVKRRLQFRLEKVLERIHILEGLLIAYLNI
                     DEVIAIIREHEQPKQGLISRFNLTERQAEAILEMKLRHLAKLEEIKLRGELDELSDER
                     DTLQKTLASHSLLKELVKEEITKDRDEFGDPRRSPIITREDSQALKEEDILPNEPITV
                     VLSKNGWVRAAKGHDVDGKELSYKAGDEFKAQTLARTNQQVLFFDGEGKVYSLPGHAL
                     PSARGQGEPLTGKLNPAEGILFEAIVGGEPEQKVLLATDSGYGFIAKIEDLYVKNRNG
                     KACIKLSENSHILPPRLIPNREQLFVACATNVGRLLIFSLEELPELSRGKGNKLISIP
                     VAKAVSREEYIVDMQVLTTNDSLTVHAGKRHFTLKGEDLTHYKGERGRRGNRLPRGLQ
                     NVSQLHVTQSE"
     gene            complement(15562..15984)
                     /locus_tag="ACF2SP_000743"
     CDS             complement(15562..15984)
                     /locus_tag="ACF2SP_000743"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFD2358545.1"
                     /translation="MSITTHLDIVSAEHEIFSGVVEMVVATGELGEIGITPGHAPLLT
                     VLRPGEVRITLQGGTQDIYYVQGGMLEVQPHCVTILADVAERAEHLDEAAALAAKAKA
                     EAAIASKGGDIDYSVAAAELARAVAQIRAIQKTRKKMK"
     gene            complement(15996..17372)
                     /gene="atpD"
                     /locus_tag="ACF2SP_000744"
     CDS             complement(15996..17372)
                     /gene="atpD"
                     /locus_tag="ACF2SP_000744"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFD2358546.1"
                     /translation="MSLGTVVEVIGAVVDVEFPRDSVPKVNDALKLVDSDLVFEVQQQ
                     LGDGVVRTIAMGTTDGLKRGLKAENTGHPIQVPVGKKTLGRIMDVLGRPVDDAGPIDA
                     EETWAIHRKAPSYEEQAGSQELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRV
                     ALTGLTMAEKFRDEGRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGM
                     LQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIVGQEHYDTARRVQQTLQRYKELKDIIAILGMDELSEEDKRVVTRA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIKGFQGILAGEYDDLPEQAFYMVGSIEE
                     AVAKAKTL"
     gene            complement(17385..18251)
                     /gene="atpG"
                     /locus_tag="ACF2SP_000745"
     CDS             complement(17385..18251)
                     /gene="atpG"
                     /locus_tag="ACF2SP_000745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFD2358547.1"
                     /translation="MAGAKEIRSKISSINKTRKITRAMEMVAASKMRKTQERMRASKP
                     YANKIYEVIKHIARAASEYRHPFMSEREIKRIGIIVVTTDRGLCGGLNSNLFRETIRT
                     IRNWQEHGKEVDIAVIGRKGQAFFRRVGGNILGSIDHLGDTPSINDFIGVVKIMLDAY
                     YNGAIDSLHIVYNEFINTMTQKPFVKQLLPLPKSEEDKKTLGHHWDYIYEPEAKELLD
                     EILERYIELQVYQAVVENIACEQAAKMIAMKSATDNAGDLIKEFQLAYNKARQAAITQ
                     ELAEIVGGAAAL"
     gene            complement(18341..19894)
                     /gene="atpA"
                     /locus_tag="ACF2SP_000746"
     CDS             complement(18341..19894)
                     /gene="atpA"
                     /locus_tag="ACF2SP_000746"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFD2358548.1"
                     /translation="MSEQVALNPSEISELIRKKIDQFSVVSEARNEGTIVSLKDGIVR
                     LHGLADVMAGEMIEFPGGVYGLALNLERDSVGAVILGDSSTLAEGQKGKCTGRILEVP
                     VGKGLLGRVVDALGNPIDGKGPIESSGMSPIEKVAPGVITRKSVDQPVQTGLKAIDAM
                     IPVGRGQRELIIGDRQTGKTAIAIDAIINQKGTGVKCVYVAIGQKASSVASIVRKLEE
                     HGALEHTIVVVAGASDSAALQYIAPYSGCTMGEYFMERGEDALIVYDDLTKQAWAYRQ
                     ISLLLRRPPGREAYPGDIFYLHSRLLERAARINADEVEKLTNGEVKGKTGSLTALPII
                     ETQAGDVSAFVPTNVISITDGQIFLDVDLFNSGVRPAINSGLSVSRVGGAAQTKIMKK
                     LGGGTRLALAQFRELEAFSQFASDLDDATRKQLERGQRITELMKQKQYSPLTVAEMGV
                     SLFVVEKGYLDDVPVNEISSFEASLHDYMRSTHAALLHAINEAGAYDNEIEAKLKKAV
                     EEFKNTGSW"
     gene            complement(19912..20454)
                     /locus_tag="ACF2SP_000747"
     CDS             complement(19912..20454)
                     /locus_tag="ACF2SP_000747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFD2358549.1"
                     /translation="MSDSTTIARPYAKAIFEHALAEKKLSEWSEYLTLLAQVVLTPQA
                     TQFIANPASTDEQQIELLIEICGSKFKKNDALNNLIKLLTTNKRLMLLPEIKALYEVY
                     RAEQEKILEVDVVSYSELTPAQQQRLSESLSQRLSRKVSLKISIDPSLLGGALIRAGD
                     LVIDGSVRGKLNMLGTSLAA"
     gene            complement(20456..20926)
                     /locus_tag="ACF2SP_000748"
     CDS             complement(20456..20926)
                     /locus_tag="ACF2SP_000748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFD2358550.1"
                     /translation="MDINLTLIVQMLVFAAFVLFTMKLVWPPLAKALEERQDKIADGL
                     AAAERGRKELELAQHRVKDELKQAKAHSADIIDKANKRASEIIEAAKEAAKREAQIQA
                     KLAQEQIAQQVNHAKEELRKQVAKLAITGAEKILMREVDAKANSELLDNLIEEI"
     gene            complement(20978..21253)
                     /gene="atpE"
                     /locus_tag="ACF2SP_000749"
     CDS             complement(20978..21253)
                     /gene="atpE"
                     /locus_tag="ACF2SP_000749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFD2358551.1"
                     /translation="MQAAELIAQVQSMTVIAVALLIGLGALGTAIGFGLLGGKFLEGS
                     ARQPEMVPMLQVKMFIVAGLLDAVTMIGVGIALFFTFANPFLSSLGS"
     gene            complement(21294..22121)
                     /gene="atpB"
                     /locus_tag="ACF2SP_000750"
     CDS             complement(21294..22121)
                     /gene="atpB"
                     /locus_tag="ACF2SP_000750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFD2358552.1"
                     /translation="MVSSTNYIKHHLTYLTYNVNDMTFGSSGGFWTLNLDTIFFSIAL
                     GLIALAIMYLAARKVTTGVPGKLQNFAELMLEFADNQVKDCFHGKNNLIGPLALTIFM
                     WVFLMNFMDIVPVDILPVLAQLVGIHYLKVVPTNDLNLTFGLSLSVFFLIVFYSLKIK
                     GVKGFVKELTLQPFNHPLFIPFNLLLELVGLIAKPISLALRLFGNLYAGELIFILIAL
                     LTLNATTSSLISTMTLGTAQFILALAWSIFHILVITLQAFIFMVLTIVYLSLAHEDH"
     gene            complement(22123..22518)
                     /locus_tag="ACF2SP_000751"
     CDS             complement(22123..22518)
                     /locus_tag="ACF2SP_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFD2358553.1"
                     /translation="MNKQLNKRGITRLWLAQLGVTVILAGLCAILFGVNAASSALLGG
                     LVCIIPNAYFAIKLFKHQGARAARQIVNSFYKGEALKIILSMFLFALVFLLCRITPAA
                     FFASYILVLMTHWFAPWIIVNKQNRPKSD"
     gene            22672..22917
                     /locus_tag="ACF2SP_000752"
     CDS             22672..22917
                     /locus_tag="ACF2SP_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358554.1"
                     /translation="MSEKIQHTAARLQEKMSNRYSDGLSHKHNKNHLSYRQHCRSHPC
                     NQTNNKDEENIVNGSTGLLREFNLRKIRKRLDKGRFH"
     gene            complement(22987..23574)
                     /locus_tag="ACF2SP_000753"
     CDS             complement(22987..23574)
                     /locus_tag="ACF2SP_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFD2358555.1"
                     /translation="MFKHGCFILIVLFLASCLSGCLGALWTGATLAYDRHNVYKKLDD
                     YNLIKALNDVLAVNRTFKNSETVLDIAVFNRDILIAGHVPTQELYDELQLRLSKVKGY
                     RRLFNRVIINKMPSNSIQDSWITTKIRSQIFADSSIDPNAFKVVTSDRVVYLMGDVQT
                     EQAEKVIEIARYTSGVQKVIKLMRYYTYQTSTNMA"
     gene            complement(23576..24148)
                     /locus_tag="ACF2SP_000754"
     CDS             complement(23576..24148)
                     /locus_tag="ACF2SP_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFD2358556.1"
                     /translation="MKMNLKFKSIIVMMIGALLTGCVAVVVAGAAAGMVYDRRSVTTM
                     EADARLFHVIHKTIIKDPRFRHSRILVSSFNRVVLLVGQTPAASLRVLAERIAQNAPG
                     VKRVYDEITVDNPIPLTQRTKDSWITSQVRSNMLTKKGLESGSIRIVTENGVVYLMGI
                     VSDEQALLAVDVARRVAGVRKVVKIFQYIR"
     gene            complement(24152..24748)
                     /locus_tag="ACF2SP_000755"
     CDS             complement(24152..24748)
                     /locus_tag="ACF2SP_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD2358557.1"
                     /translation="MQLEERVRHLFGVTIEAKISAADMLSGSIAKAGKLLVNCLLNDG
                     KIFICASGGSSANCMHFSSSMLNHFEVERPSLPVINLCADASCLSSFADEHHFGQVFA
                     RQIQALGQEQDVLLLLTTSGNSDSMLQALHAANERGMDTIALSGRDGGVLANHMGPED
                     IEIRVASDSTALIRELHLFILHCFCDLIEQSLFGQVLG"
     gene            complement(24778..25134)
                     /locus_tag="ACF2SP_000756"
     CDS             complement(24778..25134)
                     /locus_tag="ACF2SP_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFD2358558.1"
                     /translation="MTQEKGKFAEQLALNYLKENGLALVMQNYHCRLGEIDLIMREGS
                     YLVFIEVRSRSNMNFGGGLASITYEKKQKIIKATSHYMIKYRIQDKFPIRFDVISIDG
                     KSNKITWLKNAFDAGC"
     gene            complement(25156..26967)
                     /locus_tag="ACF2SP_000757"
     CDS             complement(25156..26967)
                     /locus_tag="ACF2SP_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HFD2358559.1"
                     /translation="MLIKQLKLRMFLVLVSLFLLCQCTKTVNSTEPVVTVNKKLKTPY
                     SLPTAAYLAMAKSQQGYEKQSALISAAGRLITDGQWKQGTAILAQTGELTVEQADEKN
                     LLLAKVDFMRDRPRDALAKLARIKEPERLSLYNQIQFHEQLAKSFRSIGSYSESVSER
                     IKLESLLPDEESQVNNRRALWLTLTSLPQEELNTMALEAVNKSEMQGWLQLAVISRKY
                     RNNSKSLLAALDQWQMHFSNHPANQILPNPLDSISDKLLAPPKQVALLLPLSGPLSGP
                     GNAIREGFMAAYKANRGDESTKIKVYDTSKGDITNTYRQAISDGAEYVVGPLTKTQVS
                     TIASMEHPVPTLLLNDTDASTQNNSYSLGLSPVNEAIQVAIKARSKGYKKALIIAPNN
                     AWGNEVTKAFINQWNEDGGHIVDSLRYEAKQDLNKSMKDFLQITNSQEREKKLKQVLG
                     YNFQSTTSRRQDFDMIFLLAYPSKARQIMPLLKYYYAGDAPVYATSSVYGGSANALKD
                     KDLDGIIFCDMPWVFSHQMGTKNWPEQFNSYNRLYALGVDSYTLATQLNQLILFPADG
                     SSDSTGILYLKPTQQVARVLEWGQFKQGLAHSLGDTV"
     gene            27007..27858
                     /gene="rsmI"
                     /locus_tag="ACF2SP_000758"
     CDS             27007..27858
                     /gene="rsmI"
                     /locus_tag="ACF2SP_000758"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFD2358560.1"
                     /translation="MTNTLATGLGTLYIVATPIGNREDISFRALNTLKSVDLILAEDT
                     RHSMQLLTSLGIKNNLTSLHAHNEANKSNEIIELLLHGKSIALISDAGTPLISDPGFP
                     LVKQARQHHIPVVPVPGACALIAALSAAGVPCDSFAFLGFLPAKQSARKHALESSQSL
                     PYTIVFYESTHRIIDCLNDIAEIYGQDYELVLAKEITKTFERFVSGKIKEIKDWLLSE
                     PGHIKGEFVLIFPPRSTNKDLNSHEELLKILLEELPLKQAVAIACRLTNANKNQLYEE
                     ALKLKES"
     gene            27988..29055
                     /locus_tag="ACF2SP_000759"
     CDS             27988..29055
                     /locus_tag="ACF2SP_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HFD2358561.1"
                     /translation="MDDLKKYSFLLVYLLIPLPFVSLYLGGWYSFLPFFIVFAFIPLI
                     DAFLHDSVNPTKEQEQELLKDKFFKYITCAYVPIQITIIAYGVYIVSTQNLVWNEWLG
                     FTMSLGLISGGVGINFAHEMMHKNSKLQQLMSKILLVTVCYGHFFIEHVKGHHVKVAT
                     PDDPATARLGENLYQFLPKTLLGSFQSALLLEEKRLSHKNYCIWSIHNNFWWILTVPF
                     IICIACFIFGGLIALSFFLLQSFIAILTLEIVNYIEHYGLERKMLSNGQYEKVNPNHS
                     WNANHWLSNQLLIHLQRHSDHHAHGARPYQILRHMENSPQLPSGYLGMIIIALIPPLW
                     HYIMDKRVVAYRIQLRQLEQL"
     gene            complement(29106..31271)
                     /locus_tag="ACF2SP_000760"
     CDS             complement(29106..31271)
                     /locus_tag="ACF2SP_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFD2358562.1"
                     /translation="MTRSQLILAILAGLVISIDNMISIVAFSSLIYQGALEIYIPLVI
                     NILAISVIIISASYLFMSSPPYAIAQLQDEAAILYSAMALVIIKSMPSSSTNEAIFIT
                     ILFMLGVTTCLTGLSFYFVGKFRFGNIIRYLPYPVICGFMAATGWLVLSGTFALLTGK
                     ALHQNWLVFFHKEEILLWAPGFIAGIVVYIFKEKLGYRYALQLMFISLVILFYALFYI
                     FNLEYLVDPTEGYVLGPFNQNHIDSLMKPEIWQMIHYPFSMTLLNYIGLVILVSFIAL
                     LLNASSYELIVNKQLDLNKELRIAGIGNVFSGLLGGMVGYLSLSASTVAKEYGGARII
                     GVMAFVPMLLILCFGAVVVECFPKMAIGCYLFYIAFCFLSEWLINTWRLLNLSEYLIV
                     LLILVIAIVFNMITAVAIGTVISIFIFAFRYSKINPVKYLFSGSDYNSSFERNPISQS
                     ILSTQGDEIQFLKLQGFLFFGSIYRLLQLIKSIQKARYIILDFELVYNIDSSRIILMK
                     NLKLHAEKNNISFAICSLKPIVYHELMKTNLLQSKTNWLKVFTDQESALEWCEDQVIE
                     KYTENINIEEVTLEKQLLYLGFSSELVAWISEKASIKPYNPDDIICHEGEESNSVYFI
                     HRGKVTAFVGDKRVLVVGSGNVLGEIAMYTHKKRTATLKTNEKTIVYEIDLKSIQELS
                     KNKPLLLVQFHMCMAKILANRLSVLNKRIKVFDSTAILQ"
BASE COUNT         9723 a         6611 c         5518 g         9852 t
ORIGIN      
        1 atttaattct ctatagagac tgtttttgga atcaacaaat cccacgccaa aatgttgatt
       61 ggcatcttgt aaatatttta attccctatt agtcaataat aaataattac ccagtacgcc
      121 tccatacctc tccttgatgc ttaattcaca aaatttctta acgttattat gcactgcaac
      181 aagcaataaa gattgcctag gaaaagaaca caatttttta aaggtagatt cctgcaaagt
      241 caataaatga aagtctttac atggcgttaa atgttgcagg gtttgcacta atatctcttc
      301 ctcctcaaca tctgttttta attcaataaa taaatgcatg gaattcccat actgacgcac
      361 tacttcagag agcgacggga ttctgcactc caatgcccgt aactcactaa aattcagatc
      421 ggcaatagca atgtccttgt tccataatcg atttaaggta ggatcatgat tgacaacgaa
      481 caccttgtcc ttagtgaggt gtacatccag ttcaattccc caacatccgg cctcttttgc
      541 gttctgaaat gcttccagag tgttttcaat tatccctctt gcattattat gagctcctct
      601 atgcgcgatt attaacgcac cgttgatatt ttcgaattcc ggtgtgtgcc tgggaattac
      661 agcgaaatac ccatcaatta ttttttctat gcatttgagt aacaaaacta attatcctaa
      721 tagtccaatt ttaatcattt tatgccggca taaaataaaa ggcaaaatac gctattagca
      781 caaaatcgca aaaatcgact aaactagaaa ctatgtgaga attacaatac aacaataaat
      841 tagcatttat aaaatttgta aataacttcc atcttaaaat caatgagttg aattgaagtt
      901 atttagaaat cctgatttaa atttcttggc tacaaatatt cacaaggagt gttctaatgg
      961 caaccaaagt aacaaaaagg gggatggact tactaagaga ccccatacta aataaaggca
     1021 ccgcatttag cctccaggaa agagatgaat ttgccttgca tggtttaata ccaacaacag
     1081 tggaaaccct tgaacaacaa gtaattcgct gccttgacgc ttatagtgcc aaagaagaac
     1141 ccttggagaa acacatttac ttacgtgcct tgcaagaccg gaatgaagta cttttttatc
     1201 gttttataat tgataatctg gttgatattt tgcccattat atacacacca gttgttggcc
     1261 aagcctgtga aatgttttcc cacatttatc ggcaaccaag aggagtattt ctaagttatc
     1321 cagagcggga caaactggat agcatcatac aaaacattgc gtcgactcgt tcaatcaaag
     1381 tgattgttgt cacggatggc gagcggatct tggggttggg agatcaagga gcaggtggat
     1441 tgggaatccc tataggcaaa ttgtctcttt acaccagttg cggtggtata catccgtcta
     1501 atactttacc tattattctg gatgtaggga ccaataataa ggagcgtctg gacgatccgg
     1561 aatatattgg gtggagacat gctcggattt caggcaaaga ttatgatgat tttgttgatc
     1621 aatttgtgca aagcataaaa cgccacatgc ctcatgtgct attacaattc gaagattttg
     1681 cacaacaaca tgcctatcct ttattagagc gatataaaga tcaactttgt acttttaatg
     1741 atgatattca aggaactgct tctgttgctg tagccgccat actggcggca actcgtgtta
     1801 ctaatactcc tttaaaagaa catcgagttg ctttattagg cgcaggatca gcgggttgtg
     1861 ggatcagtga gcagctggtg catgccatga tgaaccaggg actttctgaa gaagaagcca
     1921 gatcacgttt ttatttagtt gacaggtatg gacttttaca tgatgagatg acagatctat
     1981 tgccatttca aaaaggattt gtccgctctt ctaccagtct ccaaaattgg aaattagaaa
     2041 aacaaggaga aatcacctta acagacgtta ttaacaatgc ccaaccgact atactcctgg
     2101 gtgtgtctgg ccaacctaac caattcaagg aagcaatgat caagaccatg ctaagctatt
     2161 gtgaacgccc cataattttt cctttatcaa atcctacgtc tcgtgcagaa gcaatcccgc
     2221 aagatctgtt aaattggaca gctggtaaag ctttaattgc aacaggcagt ccctttgagc
     2281 cagttgcaat taatggtcat aaaattgaga tagctcaatg caataattct tacattttcc
     2341 cgggagttgg tcttggagta gttgcaggac aagccaaaag agtgactgat ttaatgatga
     2401 tggcagcagc agttgcatta agtgagctag cacctgcaat cagaaccgga gagggacgct
     2461 tactcccaga attaaattcc attagagaag tcagtcaaca tatagcgaga gcagtaattt
     2521 tacagggtat aaaagaaggc catataggac caatgaatga taatgaaatc gatgattcta
     2581 taaaaagaac gatgtggact ccacaatatg aaccatatgt tctataataa accgatgtag
     2641 aaattgctgg aatttaagtt ttaatttttt agaaagacta aaatttatta gtttatcgca
     2701 atagaattta cagcataggg ttgagggaaa atcaggttat tgatcctgcc tgattccccc
     2761 cagtttattt atggaagctt attcttatta ttaaattgag cttaaaaaca gataggaaga
     2821 ccctgaaaca taactatttt ttatcgagcc ccttatatgt ccataattac taccaatctc
     2881 aatcgggcaa ttcatgattt acagcaagga aaaccagtag ctataccgac tgaaacggtt
     2941 tacggattgg cagcaccaat aaacaataat gctgccatca gagctgtgtt tgaaatgaaa
     3001 agcaggcctt taaatcatcc tctgattgtt catattgctt ccaattggga tctgcatgac
     3061 ctcgtggaag aggtacctga gtatgcgcat caattagctg acaagttttg gcctggacca
     3121 ttgaccctgg tattccattg taaaaaagat caaatcaatc cgctcattac cggtggtcaa
     3181 tctaccatag cgataaggtg cccggatcac cccttgacac aaaagttgtt aaaaaaatta
     3241 aatattcctg tggtggcacc ctctgccaat ccttttggaa aaattagtcc gaccaccgct
     3301 ttgcacgtta aacaatcatt taatgatgcc gaattaacta tattagacgg aggacgctgt
     3361 cgtgtgggaa ttgaatcaac cattgtagat gctaccaatc ctgattctta tcaaatttta
     3421 cgccatggga taatagatga aaaaactatt gctgaaacaa ttgccatagg ttgctcgact
     3481 caaaaaaatg atattcgtgt ccctggtaaa ttggatagcc attatcaacc acaaaaaact
     3541 ttatattatt ttgatcaata tgaaaccctt tccgaatttt gtcaaaaacg caaagggact
     3601 gtttatgtca tagcgaatag acaaccccca ggtgtcgaga ctaatctctt tcatccactg
     3661 ggaatgcatc ctgaaaaatc ggcctatgat ttgtattatc aactaagatt agctgataca
     3721 tccgatgcca tttgtatcgc tatcgagcta ccacccaata cgagtgaatg gcaaggagta
     3781 cgagaaagaa tattaaaagc cgggttcccc tattcgcagc aataaatctc gagtaattta
     3841 tacttttttg atttcgcgca atagtgtatt gacagaacac tataatttgc gtaacttaac
     3901 gccctatgcc gaggtggtgg aattggtaga cacgctagct tcaggtgcta gtgggggcaa
     3961 ccccgtggag gttcgagtcc tcttctcggt accattaatt aagtaatgat tgccattggt
     4021 ttacccaact gaaccttgct accagccaat aattctttat cccattgtat tttttctcca
     4081 tttgcaaaca atagaacgac cgtagatccc aatttaaaat aacccatttc acttccctga
     4141 gatataatct tgtctgaaaa ctgctcacta taatcaaagc gttctaattt ttttgatctt
     4201 ttgacatcac catgccaact ggttccaatt gcgcctacaa tagtagcccc aaccatcacc
     4261 atcaccatcg gaccaatttt tgttttaaaa aaaatggcaa gcctttcatt tcgtgcgaac
     4321 aatttaggaa caacacgtgt agttgctggt tgaacggaaa acaaagcacc tggaatataa
     4381 gtcattgaaa cgagctcagc atctattggc atatgtaccc tgtggtaatc tttcggcgat
     4441 aagtatagcg ttgcgaattg tccctgcaca aattgttcgg ccaattgccc atcacaggct
     4501 aaaagctctt gtacagaata atatttgcct ttagcctgca ataactgtcc tcgttcaatc
     4561 ttgccgattt cgctgataca accatctact ggacaaatca catctgactg agaaagaggg
     4621 cggcattccg gctttaaatg acgaataaag aaatcattaa aacagtcgta agacttagga
     4681 tcttcaataa gtgcctcact catatcgaca tcaaacttac gaataaagtt tcgaattagg
     4741 aaattcttga gacgagggtt cctaacgtca gcaaaatacc ctgctaaagc agtgataccg
     4801 tgtttcggaa taaggtattg gggtagtgtc ttcagtacat cacgaaacat actttttact
     4861 caaaactgaa aaaagagaat aataacctaa agcttattgt attgtcacaa taagacgagt
     4921 aaatctccat gccagagaaa atttagacca ccaagctcgt aatatttgaa ttcaaagtag
     4981 tttcttgcct atgagataca ggttctgcgt aatttttgac ctctttaaaa taatcagccc
     5041 tgtgttcttt ggcattgtag caattaatta gcctgaatcc tggtaccttt aatttttctt
     5101 tatttttgtt tatgtctttt tgtatagccg cgatgatttt atcaaataaa acatcattca
     5161 gagaggagga gtctttcccg ttcaaaaagg tcatagcaac aatttttgct tgtgagaatt
     5221 ttgtacaata accttccacc tccgatctcc ttgaagtgga ccaccataat ttaccttcgt
     5281 aatcttttaa tgcagactca agcaacccct taaacttagt cgcggggagc tcatcaatcc
     5341 actcatacat tgttttgaaa aatagagact taccctcaag attgtataaa tgaataaaac
     5401 gcctgtcatg tttggcgaaa tctacagctg ctttggtaaa aatttccaaa tccttatcaa
     5461 aattaccttc tttaactgca ttacgaatgc tcaacaaagg ataagtttgt agcatataag
     5521 gcgtgaggtg ttgaataagc aaagtattta aagcacctgt atcaccttcc cctgcgctta
     5581 aaatataagc caatctgttt tcaccacttt catttgcgct ggcttgtaaa tattctttaa
     5641 caggttgtgc tctatgacgc tttgataaca gttctatagt tggggcataa tatttatcga
     5701 ttatttcaga gatatgatga gataaatctt ccggtcttaa gttcttggcc caaaggaata
     5761 aagaatcatt aaactgccta atgacgtctt catgcgtatg cggcaatatt tctttgggag
     5821 tcgtggctac agttgcttcc ttcatctgct catctgttgt cgttaaatgc aattggaaat
     5881 tggcccgctc tgtgtattgt tttaacccaa cggctatacg attaaattca ccggcaagag
     5941 tgcttgtatg tgccaaactt tgacgaattt tcaggttata attcgtatac aactgtacca
     6001 attgctcgga gaattcaaga ttgtcctcct ccgttaaatc ctttcggtct ttctgataat
     6061 aagtcttagc tactgcatga aattgagttg taaattcaac aagcaatatt aacgctgttc
     6121 ttagtttgct gtctttatct acactaatta atggggtaat tttctcgagt gataactcag
     6181 gaatggtgcc aaacaactcc caggcattgg ttaaggtgtc atcggttggc tttttgcctt
     6241 ctacttcatc cagaacaacg tgaaatgtag acacttgctg caatagttta ttagtaaggc
     6301 tataggagtc atgcaacaga tctctgaatg taggcgcata agcatctcgc cacacctgat
     6361 gatatcttct tcgcaactca tcttcatcaa atttgatgct gctgtcgtcc ttgctaaaaa
     6421 tcgtaatatt ttgagtcctt gcccattcga ctatgtcttt atagaaagaa ggtttgtggt
     6481 agagcgctga attcgttgca ggcaatggaa ctccgtaccc gttattttca caccccatat
     6541 aaaaaaccac tactcgatac aaattatcat aatgcatttg atataaattc attatgaaat
     6601 cgacgaatgg ttttatattg gtgttttcat tgcatagatg aggaaatgtt ttctcgtact
     6661 gactgcgcag ggcgacatcc gtttcatcca gagaagtgta gtttagcgtc atctcaccaa
     6721 aaagctcggt caaacgtttt cttatcatct ccggctgata agttaacaat attttcaacg
     6781 cggcagcaaa tttttgctcc tgagccactg gctcatgagc gagttgctca aattgaccag
     6841 gatccgggta cgtcttttca agaattaaat taaccagctt atcttgaaca ggcaaaaccg
     6901 ttggcaaagt ttcctgcccc ggatttttat atgttggcca atgaaaaggc tttgagtctt
     6961 tgacattagg aaatccttcc caatcacgca cggtcaaatt aacccttgtt ttaggtatac
     7021 ctatagctgg cctgggctct ttcatgtaga tggtaaacca ataaaaaaac atatcaaaat
     7081 caatgtcccc ggccaaactc agattatgcg ggtgaccatc atcgtcatca agaaaccacc
     7141 ttcccagaag gatttccatg aaactttttt caattaaggt tcttgtgctg ggaatcacct
     7201 gttctttggc ggaagactcc tgaggcactg actctttatg gaaattaaac cctttaaatc
     7261 cgtcaacact gatagataaa gtacctacca gcctctcttc atcatcaaat acaagacgtt
     7321 cttcggccga tctttttccc tgaaaaattc ttttaaataa agagacagcg acacttattt
     7381 tcgctaaaag ttccggataa tgatttttag gatccagttt tttataaaag gcatttttaa
     7441 taacaccatt ttcaataaaa ctgaccttgt atgtttgatg cgagctatcc gaaaccgcag
     7501 tccccgctgt taaaaactgc agttggctct ctttcagtgc ttttttgggc aaacccatat
     7561 atcaaatcct ttaaatcaga tcaatccatc ctatgcccca tattaatcag aaatagtcaa
     7621 gaagttcaag tataattttc atacaaaata acacttgact agaaacaaac tagacatctg
     7681 gcacacataa tgagttataa ataaaaattt gcttgtttat ttatcatgat gacaaacatg
     7741 aagccatcag tttaattaaa cattcagggt tgtcttatat tgcccaaagc gataagggtt
     7801 tgttgacaga agccctatga ttagggcgaa attgtaatta actccggaga atcaatagta
     7861 gttaatacct ctttatttaa ggctcttatg acttctccag atggactttg gttaaaaaac
     7921 caataattgc cagtggcact ttcttttaaa tcgttagcaa aacccaaggt cagtgccgta
     7981 ataataatat tagctataac gactaaaact ttatgtatta ttgactttat ttcaggatca
     8041 acaatactca atatttcctt ttccgcactt ttcattttaa tgtgcagatc aacacctgat
     8101 tttgttatgc catcataggc aatgctgtag agcgtttgcc ggtatttttc atcagcacgc
     8161 tgccaatttc caactttatc tttatcaaga gatgattttc gtaatctttc atgtatatcc
     8221 ttgcattttt cttctacccg cataatgaca tgcctgaaac ggctggagtt gatattgttt
     8281 tcttcgcctg taaatgcaat gatctctttc ttaaatccta aatcttgcat ctgctggaca
     8341 caaataaatc gttcgcgcaa cgctcgtgcc aaaactaata aattgctgtc tttaatttgt
     8401 aaaagcgcct ttccctgagt aacaaccccc ttttgagctc ctatatataa aagctcaatt
     8461 aaagcttttc tatgagagag cgattcgatt ttagcgagtc ctggcaaaaa caagcgaagc
     8521 aattgccctg ttttattatt ttttagcacc aaacgataag cataatcatt ggcaatcccc
     8581 gcttctttca agactttaaa agatttccct aaagcatcca gatcatccaa atttaattgg
     8641 gataaatcgg atttacgcaa attgtaggct ttaaattgtt cagagtaatg atataaaata
     8701 gattcctgtt gatgtttttt gggatccaga gccagttttt ttagctgttt tatagaaatg
     8761 gttttggttt tatctaatgc tatcaatgtt tcagccagca ttgggtaatg gctggtagcg
     8821 ttaacaatgt cttgtatttc tttaatcgat aaactcccct tgacccaaaa tataagacat
     8881 agttttctgc aatgatcatt tgctaaagta ttaatgaatt ttagttgctc tctcttttcc
     8941 tgatgggcta ataattcagg gatatcttga gttaaaccaa aatcgacaag gacatgaagc
     9001 gaacgataat acgcttcgtc aaataagagc tctttaatct cacttaaagt gaaatttttc
     9061 tgaattagaa aaggaattaa cttgatttgg atgtcatccc acatcaatgc gcttaattgc
     9121 tttattaact catgatcttg caataaataa cgattttctt ccaaagtatt ttcttcataa
     9181 aatacaataa tcatttgcaa aatactttta tacagagttt cctcagagtt aaaaggaacc
     9241 gcttcaagct ccttctttaa tctgctttct tccgacaata aatcgttttc catggcggga
     9301 gtcaaacgaa gcttgtattg ttttaccaac tcctgggctg taatactcat tggaagttaa
     9361 cctcttgttc atttactaat taaccctatt tcatggagat agggtaataa agattatttt
     9421 ttggatcaaa atttggttat tttgaacgat acataaatag tatcaaaact acaaaatgta
     9481 aatattataa ccaattttgc agcaaatggg gatttttttt aaaaataaat tgagttttat
     9541 tccctctctg tttttgagga ataacctcct caacgtagag aaaccttggc tcggatgtgg
     9601 ttgctccttt gctacctatc cttctcatgg ctccggctca gctctttgtt gaactgagcc
     9661 tcgcccttta gagtacagca gcgagctcta aatacctgga acaattatct tgcccagtta
     9721 gagttcttga atagaaatga tcaaccttcc ttagcaactc tcgttgtttc aagtagcttg
     9781 aactgaagtc aaacaactta aagctagctt ttatgcatcg gacaaacgac tcccacctcg
     9841 gcaaatgcct cgataacagg tgttttatcc caatgcttat ctatggttgc ctgaatgacg
     9901 ccacatgcag caaaatcata gtatgcaata ggagaccaat aattcatatt ggcatcaacc
     9961 ataacctgaa atgctttttg tatagaccag cctggtttat gtgctaataa ataaaatgct
    10021 ttattaaata ctccgctcgt tatatgcaca tccaacgcat cagtgtattc actagcatgc
    10081 cctatagaca ttccatcctt ggctggatca tccaaataac gaataggttg cccacttttt
    10141 acagcttcac gcccaatggt ccaatcctca ccatcccaat accatggata atctttacta
    10201 agataatcta acaatgcaat tgccgccata tcagagaaag attcattgat ccccccagat
    10261 tgcccttcat acatcaatcc ggaatgcaag gcagtaaaat tgtgcgataa ttcatgtccc
    10321 aatacactct gggcgggagc tgttaaaaat tcatcaccat ttccgattac aatttgctga
    10381 tgtgcaagaa ctaccccatc aagactgatg gttggtactg caaaagcgtt atccatatca
    10441 ccaagatggg tgtaagcccg tataggtaaa tcatcaccta ttggacgatt aacaccataa
    10501 attttttgat acatatctaa cgtttgttgg gcaaaataca tcgtatcgtt aactggagaa
    10561 aaagcataat tgacaggacc tgttctgcca tcagcatagt ttaaaaacaa gttggtttca
    10621 ttacatgggt aggaaaaggc acttaaattc agtacagact ctgcaaatat agtaattgga
    10681 aagaagtcat aacctatatt atgattttcc aaattcatta cttttatgga ttcattttca
    10741 acacgacata atccatcctc aacattaaca tcaaacttcc ctaatgaggg aagccccggc
    10801 aaagcattac catgctgaaa cattccctga cggtatggga gagtaaacgc attaccgcct
    10861 aatccctgac caattctttc atgacgtacc tcatcccatt gtttcaaaat ctcaccgcta
    10921 ttggcatcaa taagataatt aggtgcattc acatgttttt tttcactatt ggaatagtaa
    10981 gacaaatgat aagccaaatg agctttattt cctttatcta tataaataac ctttttaagg
    11041 tttttaaatt taaccggctc agctaccttt ccaagaatct tttgttctat atcattcggg
    11101 ctcaattttc cctcagtaga attaatatcc tgttcaattc cggtaatatt catacctgtt
    11161 acagtttctt tagtgccatc tttggaatga aaaatgactt gataacccca aaccggaacc
    11221 cccttgtagg ttatttgata acgagcatgc ctggtcgcat ggttagaatt attttgtaac
    11281 tccagtttat aatcactttg ctgacttttt acagtttgca ataatccttg tttcttaaaa
    11341 ggatgtgctt gcataaattg aggtaatact ttcgttgact caccccatac gattgcttgg
    11401 gttgcagcgt ttacagatga aatagctgtt gaaatgacaa ataatgaaac aatccgtttt
    11461 ttcataatat catcctgatt ttttgtggaa acttcatgct gctacaaatt cgagaaaaaa
    11521 acaatcagaa tacacaaatt acgacataat tttatttttt tatatctaga atgacaattt
    11581 atactcaact tcttatcgca aacgactccc tcagactctg ctaatgtccg cttaattcag
    11641 actgataatt tagcaagaca ataaattatt ttccattata ataagaaaat gtactgagac
    11701 agatattggt ttcactatga attttaatat tattggtgct gggcgtctgg gtaagaattt
    11761 aggcattgca atgtctgttc atcagattgc ctctttaaat tctatttgta actcaaattt
    11821 agcaagtgcc aggatagcct gcaatgaaat cggatttggc aatgcggttg gtaaaatatc
    11881 tgatctttct gaagcagata taacgtggat aacctgtagg gacgactcca ttttggatgt
    11941 agtatcgata ttggacaata ttgagatatt aaaaaaaggc agcttggtga tccactgcag
    12001 cggggctctt aattcaagtg tacttcttcc tctgaaaaaa cagggttgtt atattgcaag
    12061 cgtgcatcct ttgaaagcat tccgcactaa ttctttggat tccaatgcgt ttaagcaagt
    12121 ccattgcgtg atagagggag attatgaggc atgtcgttgg cttaggtttg cttttgagaa
    12181 gctggaagcc agcattatcg caattcaacc tgaaatgaag gtcatttatc atactgcggc
    12241 aacgattgct tctaattatt tagtgacttt agcttcatat agtgaagagt tacttttaaa
    12301 agcgggaata cagcaagcac aatcaagaaa aatgatatgt gatctcatgt acagcaatat
    12361 ccttaacttg aatcaggtag atagtgcaaa aagtgcttta accggacctt tgactagagg
    12421 agatatgcaa accatttcct tgcatctcga agcgatagat aatagcgaaa tcaagagatt
    12481 atataaaagc atggcgctag ctaccctgcc tttgttagat ttatcattag atagaaaaga
    12541 aaccttaaga aaaactcttg agaaagaata acgttgcagt tattgaataa taagccagct
    12601 ctgtgcatta ttctgtttga tttcaaatca tcatataaag gtgtttcacg ggaaacatac
    12661 agtccctaac ggccatgtta ctcagattga gtaacatgca attgagaaac attttgtaaa
    12721 cctcttggca accggtttcc tctcctgccc ctttcaccct tatagtgagt caaatcctca
    12781 cctttcagag taaaatgccg cttaccggca tgaaccgtca gcgaatcatt cgttgttaaa
    12841 acttgcatat caactatata ctcttctcgt gagaccgctt tagcgacagg gatactgatc
    12901 aatttattcc cttttcctcg agataattcg ggcaactcct caagagaaaa aatcagtaaa
    12961 cgccctacat tagtcgcaca agcaacaaat aattgctctc gatttggtat tagccttggc
    13021 ggcaatatat gactgttttc acttaatttg atgcatgcct ttccattacg attttttaca
    13081 tataaatctt caattttggc gataaatcca tacccggaat cggtagccaa taacaccttt
    13141 tgctcgggct ctccgccaac aattgcttca aacaatattc cttcagcagg gtttaattta
    13201 ccggtcaaag gctcgccttg accgcgtgcc gatggcaatg catgtcctgg aagcgaatag
    13261 acctttccct ctccatcaaa aaataaaact tgctgattag tacgagctaa cgtttgggct
    13321 ttgaattcat ctccagcttt atagctgagt tccttaccat cgacatcatg ccccttggcc
    13381 gctctgaccc atccattttt agacaataca accgtaatag gctcattagg caaaatatcc
    13441 tcttctttta atgcctgaga gtcttccctt gtaataatgg gtgaacggcg tgggtcacca
    13501 aattcatctc gatctttcgt tatctcttct tttaccagtt cttttaagag agaatgacta
    13561 gccagcgttt tttgtaaagt atcgcgttca tcagacagct catccagttc accacgcaat
    13621 ttaatctctt ccaatttggc taaatgccgc agtttcattt ccaatattgc ttcagcttgt
    13681 cgttcggtta aattaaagcg tgaaattaat ccttgttttg gttgctcatg ttctctgatg
    13741 attgcaatga cttcatctat attaagatag gcaataagca gtccttcaag aatatgaata
    13801 cgctccagta ccttttccaa tctgaattga agacgtcttt ttactgtggt aagcctaaat
    13861 tccaaccatt cagtcaagat tgttaacagt cctttaaccc ttggcttgcc atcaagacca
    13921 atcatattaa aattaactcg atagctacgc tccaaatccg tggtagcaaa taaatgcgac
    13981 attaaacttt ctatatctac tcgattagag cgggggataa taaccaaccg tgttggatgc
    14041 tcgtgatctg attcatcacg taaatcctca accatcggta attttttctg ttgcatctgc
    14101 aacgcgattt gctccattac cctggctccg gatacttgat aaggtaaagc tgttatgaca
    14161 atattttgtt tttcttgagc ataaattgct cgcattttaa ttgagccatt accggactcg
    14221 tacatagctc taattgtttc actagggcta atgatttctg cttctgttgg aaaatcagga
    14281 cctttaatat attcacaaat agtcttaata tcggcttggg ggttatccag cagatgaata
    14341 cacgcattag caacttcagt caaattgtgc ggaagaatat ctgaagccat accaacagct
    14401 atacctgttg caccattcaa taaaatatta ggcaaacgag cgggtaaaag agcgggctct
    14461 tgcaaagtac catcaaaatt atctacccaa tccacggttc cttgtagcag ttctccgagc
    14521 aacacatcag cataaagaga taatcttgct tctgtatatc tcattgcagc aaatgatttg
    14581 ggatcatccg gggatcccca gtttccttgt ccatcaacaa atggatagcg atatgaaaag
    14641 ggttgagcca ttaaaaccat agcttcatag catgcactat caccatgagg atggaattta
    14701 ccaagaacat cccctactgt acgcgctgat tttttatatt ttgctgttgc tttcagaccc
    14761 aactcagaca tagcataaac aattctgcgt tgaacaggct taagtccatc tgcgatgctc
    14821 ggtaatgctc tgtccaaaat gacatacatt gaataatcaa gataagcttt ttctgtaaac
    14881 tcagtaagcg gttttttttc agttacttca ttcatattgg gtattttctt catatattaa
    14941 ttgctatcca tcatcctagt gaatcgtata agacaatgca aaacaatttt aacagaatat
    15001 tcatgctgtt agaattataa tcatcgtatt atctgtgtat aactttatat tctttttgct
    15061 tcaactatat ggattaaatt taacttattg atttataata acatttatta attttattta
    15121 attttatatc tttgtatcct gtggataact atgtgaatga atatggaata aataagataa
    15181 cttgttgtta attaccaaga attttttata agttgtaaat tgagctctcc actataaaca
    15241 aattttcaaa tttcagttgt taccacttga tttttaaatg accaattgcc cctagcattg
    15301 cctttcaacc ataatggcaa tgcgcatact ggataccata tctatgcatc cgcagatgac
    15361 ttgaattcgc tcaaataata gagcaatgct aagcattaga gttatcacac tattttcttg
    15421 gtgtattaga gagaaagata cggcaacaaa attatattta ctaagagagt aaaacacggg
    15481 tttttcctat cgggaagaaa cccgttttgt agaggggtat aaggtattca tagcaaagca
    15541 gtttccacaa acaggaaact cttacttcat ttttttcctg gtcttttgaa ttgctcttat
    15601 ttgagcgaca gccctggcca attctgctgc agcaacagag taatcaatat cgcctccttt
    15661 actggctata gcggcttcag ctttcgcttt agctgccaat gcagcagctt catccaagtg
    15721 ctcggctcgt tctgccacat cagcaagaat tgttacgcag tgaggttgaa cctcaagcat
    15781 tccaccctgc acatagtata tgtcctgtgt accaccttgc aaagtaattc taacttcacc
    15841 cggccttagc acagttagta atggagcatg ccctggagta attccaattt cacccaattc
    15901 tccagttgca actaccattt ccaccacacc agagaaaatt tcatgctctg cactaacaat
    15961 atccaagtgc gttgtaatac tcatattatc ttgcctcata aggttttagc ttttgcaaca
    16021 gcttcctcga tgcttccaac catataaaat gcctgttcag gcaaatcatc atattcacca
    16081 gcaagaatgc cttggaaacc tttgatggta tctttaagcg atacgtattt accaggagaa
    16141 cctgtaaata cttccgcaac gaaaaatggc tgagataaga atctttggat tttacgagct
    16201 cgagttacaa ctcgtttgtc ttcctcagac agttcatcca tacccaaaat tgcaataata
    16261 tcttttaatt ctttatatcg ttgcaaggtt tgctgtacac gacgtgcggt gtcgtaatgc
    16321 tcttgaccta caatcaatgg atccagctga cgtgatgtag aatcaagcgg atcaaccgca
    16381 ggataaatac ctaattcagc aatttgacga gacaatacta cagtggcatc caagtgtgca
    16441 aaggttgtag ctggtgatgg gtcagtcaag tcgtctgcag gtacatatac agcttgtatg
    16501 gaagtaatag aacctgtttt tgtggaggta atgcgctcct gcagcatacc catttcctct
    16561 gctaatgtcg gctgatatcc tactgcagaa ggcatacggc ctaacagcgc agatacttca
    16621 accccagcca gggtataacg ataaatatta tcgataaaca aaagaacgtc gcgcccttca
    16681 tcccggaatt tttcagccat ggtcaacccg gttagagcaa cacgcaaacg gtttcctgga
    16741 ggctcattca tctggccata aacaagcgat actttatcca atacattaga gtctttcatc
    16801 tcatgataga agtcgtttcc ttcacgggta cgttcaccaa cccctgcaaa cactgaataa
    16861 ccgctatgct caattgcaat gtttcgtatt aactccatca tgttaacggt tttacctaca
    16921 ccggcaccac cgaatagacc aactttacct cccttggcaa aagggcaaag caaatcaatt
    16981 actttaatac cagtttctaa taattcctgg ctgccagctt gctcttcata acttggtgct
    17041 ttacgatgaa tagcccaagt ctcttcagca tcgataggcc cagcatcatc tacaggacgc
    17101 ccaagaacat ccataatgcg tcccaaagtt ttcttaccta ctggcacttg aataggatga
    17161 cctgtatttt ctgcttttaa tcctcgcttt aaaccatcgg ttgttcccat ggcaatagta
    17221 cgcacaactc cgtctccaag ttgctgctgc acttcaaaaa ccagatcact atcaacaagc
    17281 tttaatgcat cattgacttt aggcacgcta tcacgaggaa attctacgtc aactaccgca
    17341 ccaattactt ctaccacagt tcctaagctc attatatacc ctctttataa agcggctgcg
    17401 ccaccgacaa tttctgccaa ctcttgagta atggctgctt gtcgggcttt gttataagcc
    17461 aattgaaatt ctttaatcaa atcaccagca ttatccgttg cacttttcat tgctatcatc
    17521 tttgcagctt gttcacaagc aatgttttct acaacagctt gatacacttg taattcaata
    17581 tatcgctcta aaatttcatc taataattct ttagcttcgg gctcgtaaat atagtcccaa
    17641 tgatgaccca atgttttttt atcttcttct gattttggta acggcaataa ttgttttaca
    17701 aaaggttttt gcgtcattgt attaatgaac tcattgtata caatgtgcaa agaatcaatg
    17761 gcgccattgt aataagcatc aagcattatt tttacaacac caatgaaatc attaatactc
    17821 ggtgtatcgc ccagatgatc tatggaacct aatatgttac cacccacacg cctaaaaaag
    17881 gcctggccct ttcttccgat aacagcgata tcaacttcct tgccatgttc ttgccaatta
    17941 cgaatagtac gaattgtttc acgaaataga ttcgagttta agccaccaca aagacctcta
    18001 tctgtggtga caacaataat acctatacgc ttgatttcgc gctcactcat aaatgggtgt
    18061 ctgtactcag atgctgctcg agcaatatgc tttatgactt catagatttt attcgcataa
    18121 ggtttagaag cgcgcattct ttcttgcgtt ttgcgcattt tgcttgcagc caccatttcc
    18181 atagctcgag taatttttcg agttttatta atactcgaaa tttttgaacg tatttctttt
    18241 gctccagcca tctttcaatt cgccttaatt agttttagag gccattactt tcaaataatg
    18301 gccttagtgc ctaaaagagt tagctctttg aggccatacc ttaccaactg cctgtgttct
    18361 tgaactcttc tactgctttt ttcagttttg cttcgatttc attatcataa gcaccggctt
    18421 cattaattgc atgtaataaa gcggcatggg tactgcgcat gtaatcgtgc aaggaagctt
    18481 caaacgagct gatttcattt acaggtacat cgtccaagta tcccttttct accacaaaca
    18541 agcttacccc catttcagcc actgtaagtg gggagtattg tttttgtttc atcagctctg
    18601 taatgcgttg tccacgctcc agctgcttac gtgttgcatc atcaagatca gatgcaaatt
    18661 gggagaatgc ttctaactca cggaattgtg ccagagcaag ccgagtacca ccaccaagct
    18721 ttttcatgat tttcgtttgt gcagcaccac caacccgtga aacggacagt ccggaattga
    18781 ttgctggtct aacacctgag ttaaataagt caacatcaag gaatatttgc ccatcggtaa
    18841 tagagattac gtttgttggt acgaaggctg acacatctcc cgcttgtgtt tcgataatcg
    18901 gtaatgcagt cagtgatcct gttttaccct tcacttcccc attagttaat ttttctactt
    18961 catcggcatt aattcttgcc gctctttcca acaagcgaga atgcaaatag aaaatatcac
    19021 caggataagc ttcgcgacct ggtggccgac gtaataacaa ggagatctgt ctatatgccc
    19081 atgcttgttt agttaaatca tcgtaaacaa tcaatgcatc ttccccacgc tccatgaaat
    19141 actcacccat ggtacaacct gagtaaggtg ctatatattg gagtgctgct gaatcagagg
    19201 ctcccgctac cacaacaata gtatgctcaa gagcgccatg ctcttccaat tttctaacga
    19261 tcgatgcaac cgatgaagcc ttttggccaa tggctacata tacgcattta acgccggtac
    19321 ctttttgatt gataatcgca tcaatagcaa tggcagtttt accagtttgg cggtcaccaa
    19381 taataagttc tcgctgacct cttccaacgg gaatcatcgc atcgattgct tttaatcctg
    19441 tctgaacagg ttgatctact gatttacgtg taattacacc aggagccact ttttctattg
    19501 gagacatacc agatgactct atcgggcctt tcccgtctat aggatttcct aaagcatcaa
    19561 cgacacgtcc caaaagacct tttccaactg ggacttccag aatacgacct gtacatttac
    19621 ctttttgccc ttcagctaat gtggatgagt cgcctaatat tacagcacca acagaatctc
    19681 tctccaggtt aagagccaat ccgtagaccc ctcctggaaa ctctatcatt tcacctgcca
    19741 taacatcggc aagaccatgc agtcgtacaa taccgtcttt cagactaacg atagtacctt
    19801 catttcgtgc ttcagaaacc acactaaatt gatctatttt ctttctgatt aattcactga
    19861 tttcagaagg atttaacgct acttgttctg acatggaaac tatcctctaa attaagcggc
    19921 caagctggtg ccaagcatat taagcttgcc ccgaactgaa ccatcgataa ctaaatcgcc
    19981 tgctcgaatt agcgctccgc caagcaagga gggatcaata cttattttta aagaaacctt
    20041 acgtgataat ctttgactta aggattcgct caaacgctgt tgttgcgctg gagtcaattc
    20101 agaatagctg actacatcaa cctccagtat tttttcttgc tctgctctat atacttcata
    20161 aagagcttta atttctggca acaacatcaa tcttttattg gttgttaata gcttgattaa
    20221 attatttagt gcatcatttt tcttgaactt tgaaccacat atctcaataa gaagttcaat
    20281 ttgttgttca tctgttgatg caggattagc gataaactga gtagcttgag gagttaaaac
    20341 aacttgagcc agtaatgtaa gatattctga ccattcactc agtttttttt ctgctaaagc
    20401 atgttcaaat atcgcttttg catagggtct ggctatagtg gtactatcag acattttaaa
    20461 tctcttctat taggttatct aacagttcgc tattcgcttt tgcatctacc tctcgcatta
    20521 aaattttctc tgccccagta atagccagct ttgcaacttg ttttctcagt tcttctttgg
    20581 cgtgatttac ttgttgagca atttgttctt gcgcgagttt tgcttgaatt tgagcttcgc
    20641 gttttgcggc ctcttttgct gcttcaataa tttctgatgc tcgtttattc gctttatcaa
    20701 taatatcagc agagtgagcc ttggcttgtt ttaactcatc ttttacacga tgctgagcca
    20761 actccaactc tttacgccct ctttcagcag cagccaatcc atcagcaatt ttatcttgcc
    20821 tttcttcaag tgcttttgct agtggaggcc aaactaattt catagtgaac agtacaaacg
    20881 cggcaaaaac caacatttga actattaatg ttaaattaat atccaaagta tattctcctg
    20941 taacagtgag ggcgcatagc gccctatttg tgtgttatca agaacccagg ctgcttagga
    21001 aagggttagc aaaggtaaag aataatgcaa tacctacccc aatcatggtt accgcatcaa
    21061 gcaaaccagc tacaataaac attttaactt gtaacatagg taccatttct ggttgacgag
    21121 ctgagccttc gagaaattta ccgcctagca atccgaaacc tatggctgtg cctaacgcac
    21181 ctaaacctat caacaaggca accgcaatga cggtcatact ttgaacttgt gctattaatt
    21241 cagcagcttg catattttat cccctttaca atggatgatg ataattaaat cggttaatga
    21301 tcttcatggg ccaaactcaa ataaactata gtcagtacca taaaaataaa tgcttgtaag
    21361 gttatcacta atatatggaa aatggaccag gcgagtgcaa gaataaattg cgccgtaccc
    21421 agtgtcatag tgctaattaa tgatgacgtt gttgcattta aggttagtaa ggctatcaaa
    21481 ataaatatta attcgccagc gtataagtta ccaaaaagtc gtaatgccaa ggaaatgggt
    21541 ttagctatca gcccaaccaa ctccaataat aaattaaatg gaatgaatag cgggtgattg
    21601 aaaggctgca gggtgagctc tttaacaaaa cctttaacgc cttttatttt taaactgtaa
    21661 aaaactatta gaaaaaatac ggaaagtgac aatccaaatg ttaaattcaa gtcattagtt
    21721 ggtacaactt tcaggtagtg tataccgact agctgcgcaa gtacaggaag gatatctact
    21781 ggcacgatat ccataaaatt catcaggaat acccacatga agatagttaa tgccaatggg
    21841 cctattaggt tattttttcc atgaaaacaa tctttaacct gattatcagc aaactcgagc
    21901 ataagctcag caaaattttg taactttcca ggaacaccag tggttacttt ccttgccgct
    21961 aaatacataa ttgccagagc aattagaccc aaggctatag aaaagaagat tgtatccagg
    22021 ttcaaggtcc aaaaaccacc acttgaaccg aatgtcatat catttacgtt ataggtaagg
    22081 tacgttaaat gatgcttaat atagtttgtg ctagatacca tttcagtcac tttttggcct
    22141 attctgttta ttgacaataa tccatggggc aaaccaatgc gtcataagaa ccaaaatgta
    22201 tgatgcaaaa aacgctgccg gtgtgatcct acataataaa aatactaatg caaacaggaa
    22261 catggacaat attattttta acgcttcacc cttataaaaa ctattaacta tttgcctcgc
    22321 agctctcgcg ccttgatgct taaacagttt aatagcaaag taagcatttg gtataataca
    22381 aaccagtcca ccaagcaatg cagaactggc cgcattgaca ccaaataaaa ttgcgcaaag
    22441 gccagctagg attacagtaa ctcccaactg cgccagccac aatcgcgtga ttccgcgctt
    22501 attcagctgt ttgttcacat attcacctaa ctttcacggc gcgaattata aattaaacaa
    22561 ttgtaataat caacgttgat atgcaaatta cgagctataa ttgtaatgta gggtttagca
    22621 ttaatcacat gttaagcgat ttactattta acctcgccta aggattaaaa aatgtcagag
    22681 aaaattcagc atacagcagc caggctgcaa gaaaaaatga gcaatcgtta tagcgatggg
    22741 ctttcacaca agcataataa aaatcacctt tcatatcgac aacattgcag atctcatcca
    22801 tgcaatcaaa cgaacaataa agacgaagaa aatattgtaa atggctctac agggctgtta
    22861 cgcgagttta atttgagaaa aatacgaaaa cgactggata aaggtcgatt ccattgatac
    22921 agtatactta tcctcccagg ttagccactt cctggattgg acgtgcagct attggcctga
    22981 ttctgatcag gccatattcg tactggtttg ataagtataa taccgcataa gttttattac
    23041 tttctgtaca ccacttgtgt atcttgcaat ttcaatcact ttttcagctt gttctgtctg
    23101 aacatctccc atcaaataga caacgcgatc agaagtaacc actttgaaag cgttaggatc
    23161 gattgaagag tctgcaaaaa tttggctacg gattttcgtg gtaatccagc tgtcctggat
    23221 cgagttggat ggcattttat tgataattac gcgattgaat aaccgcctgt atcctttaac
    23281 tttgcttaga cgtaattgta attcatcata caactcctgg gttggaacat gccctgctat
    23341 taaaatatct ctgttgaaca cagcaatatc cagtactgtc tctgagtttt taaatgtccg
    23401 gttgacagct aaaacatcat taagagcttt tattaaatta taatcatcta attttttata
    23461 tacattgtgc ctgtcataag ctagagttgc cccagtccat agagctccca gacagcctga
    23521 taagcagctg gctaaaaaca agactattag tataaaacat ccgtgtttaa acatgctaac
    23581 gtatatattg gaatattttc acgacttttc tcactcctgc aacacgtctt gccacatcaa
    23641 cagctagcaa tgcttgctcg tcagaaacaa ttcccatcag atagactact ccattttcag
    23701 tgactatccg tattgaaccc gattcaagtc cttttttagt caacatgtta cttctaacct
    23761 gacttgtaat ccaactgtct tttgtccgtt gagtcaaggg aatgggatta tcaacggtta
    23821 tttcatcata cactctttta actccagggg cattttgggc aattcgctcg gctagaacgc
    23881 gtaatgatgc agcgggagtt tgcccaacca gcaataccac ccggttaaaa ctggaaacaa
    23941 gaattctgga gtgccgaaat cgagggtcct tgatgatggt tttatgaatc acatgaaata
    24001 accgggcgtc cgcctccatg gtagttacgc ttcttctatc ataaaccatt cccgctgcag
    24061 cacctgcaac aaccacagca acgcaaccag tcagtaaagc gccaatcatc atcactatga
    24121 tggatttaaa ttttagattc attttcattt tttatcctaa aacttggcca aataaagatt
    24181 gttcgattaa atcacaaaaa caatgcaata tgaacaagtg cagctctctt atcaatgcag
    24241 tactgtcaga tgctactctt atctctatat cttctggccc catatgattc gccaaaacac
    24301 ctccatcgcg gccacttagt gcaatggtat ccataccgcg ctcatttgct gcgtgaagag
    24361 cttgcagcat gctatcagaa ttacctgatg tagttagtaa aagtaataca tcttgctctt
    24421 ggcccaaggc ttgaatttgc cttgcaaaca cttgaccaaa gtgatgctca tcggcaaatg
    24481 aactcaaaca ggacgcatca gcgcataaat taatgaccgg caaagaaggt ctttccactt
    24541 caaaatgatt tagcatcgaa ctggaaaaat gcatgcaatt agcactggat cccccactgg
    24601 cgcaaatgaa tatttttcca tcgtttaata aacagttaac caacaacttc cctgctttag
    24661 ctattgaacc cgacaacata tcagctgctg atatttttgc ttctattgtg acaccaaata
    24721 aatgccttac tctttcttct aactgaatca tttcaaaacc ttctattcat tatcttccta
    24781 gcatcctgca tcaaatgcgt tctttaacca ggtaatcttg tttgattttc catctatact
    24841 aatcacatca aaccgtatag gaaacttatc ttgtatgcga tacttaatca tataatgaga
    24901 agttgctttt attatttttt gtttcttttc gtaagtaatg ctggcaagcc ccccaccaaa
    24961 attcatattt gaacgcgaac gaacctcaat aaaaaccaga taagatcctt cacgcataat
    25021 taaatcaatt tctcctaaac ggcaatgata gttttgcatt actaatgcaa gaccattttc
    25081 ctttaagtaa tttaaagcca attgttcagc aaactttcct ttttcttggg tcattattct
    25141 tggctcactt caaaactaaa cagtatcccc tagtgaatgg gctaaaccct gtttaaattg
    25201 cccccattca agaaccctgg ctacttgttg agtcggcttt aaatataaaa tacccgttga
    25261 atcgctagag ccatcggctg gaaataatat cagttggttt aattgggtag ccaaggtata
    25321 actgtccaca cctaaagcat aaagcctgtt atagctattg aattgttcag gccagttttt
    25381 cgttcccatc tgatgagaga atacccaagg catatcgcag aaaatgatcc catccaaatc
    25441 cttatccttc aaagcattgg cactaccgcc ataaacactt gaagtagcgt acacaggagc
    25501 atctccggca taataatatt ttaataaagg cataatttgg cgggccttgg aaggataagc
    25561 caataaaaat atcatatcaa aatcctgtct tcggctcgta gtcgattgga aattatagcc
    25621 taatacctgc tttagttttt tctcgcgttc ctggctattg gtgatttgta aaaaatcttt
    25681 catgctctta ttcaaatctt gtttggcttc gtagcgaaga gagtcaacaa tatggccccc
    25741 atcttcattc cactgattga taaaagcttt cgttacttca ttaccccagg cattattggg
    25801 agcaataatc agcgcttttt tatatccttt gcttcttgct tttattgcaa cctgaatcgc
    25861 ttcattaaca ggggataagc ctaaggaata agagttattt tgtgtgctgg catccgtatc
    25921 gtttagcaat agggtaggaa ccggatgctc cattgatgca atggtagaga cttgagtttt
    25981 cgttaaaggg ccaacaacat attcagcacc atcagaaata gcttggcgat aggtgttcgt
    26041 tatatcgcct ttgcttgtat cataaacttt aatttttgtt gattcatctc ccctgttggc
    26101 cttataggct gccataaagc cttcccgtat agcattaccc ggcccggata atggaccact
    26161 taaaggcaat aacaaagcga cctgtttagg tggggctaat aatttatctg agatgctgtc
    26221 taagggatta ggcaaaatct gatttgctgg atgattacta aaatgcatct gccattgatc
    26281 caaggcagcc agcaacgact ttgagttatt tctatacttg cgggaaataa cagccagctg
    26341 caaccagcct tgcatttctg atttgttcac agcctccaga gccatcgtat tcagctcctc
    26401 ttgaggtaaa ctggttaagg tcaaccacaa agcccgccga ttattgacct gactttcctc
    26461 atcagggagc aatgattcta acttgatacg ctcactaact gattcagagt aactaccaat
    26521 ggaccgaaat gacttcgcca attgttcgtg aaattggatt tgattatata atgagagtct
    26581 ttcaggttct ttaattctag ctaacttggc taaggcatct ctgggtctat cgcgcataaa
    26641 gtcgactttt gccagtaaaa gattcttttc atcagcctgc tcaactgtta attctccagt
    26701 ttgtgctaaa atggcagttc cttgcttcca ttgcccatca gtaattaaac ggccagctgc
    26761 ggaaatcaat gcgctttgct tttcatagcc ttgttgactc ttagccatag ccaaatatgc
    26821 tgcagtaggt aatgaatatg gggtttttaa ttttttatta actgttacta caggctcggt
    26881 tgagttgact gtttttgtgc attggcaaag caaaaaaaga gataccaata ctaagaacat
    26941 gcgaagtttt aattgtttaa ttaacatgtt aattcgccaa atttgctttt cgcaagagga
    27001 taaactatga caaacactct agcaacaggt ttaggaactc tctatatagt tgctacccca
    27061 ataggaaatc gcgaagatat ttctttcagg gctcttaata ccttgaaaag cgttgattta
    27121 atacttgctg aagacacacg ccattccatg caattattga cttcgctagg aataaaaaat
    27181 aatttaactt cattgcacgc ccacaatgaa gcgaacaaaa gcaatgagat aattgagttg
    27241 ctgctccatg gaaaatcaat cgcactaatc agtgatgcag gcacgccctt gattagcgat
    27301 cctggatttc ctttggtgaa acaagctcgc cagcaccata tccctgttgt gcctgttcct
    27361 ggcgcatgcg ctttaattgc tgcgttaagt gctgctgggg taccctgcga ttcttttgcc
    27421 tttttaggtt ttcttccggc caaacaaagt gccagaaaac atgccctgga atcatcgcag
    27481 tcattgcctt ataccatagt attctatgaa tcaactcaca gaatcataga ctgtcttaac
    27541 gatattgcag agatctatgg ccaagattat gagcttgttt tagcaaaaga aataaccaaa
    27601 acctttgaac gatttgtatc tggaaaaata aaagaaatta aagactggct acttagtgaa
    27661 cccggtcata tcaaaggtga atttgttttg atttttccac ctcgctcaac aaataaagac
    27721 ctcaattcgc atgaagaact actcaagata ttgttggaag agttgccttt aaagcaggct
    27781 gtggctatcg catgcagatt gactaatgcc aataaaaatc aactctatga agaggcattg
    27841 aaattaaaag agagctaaaa agaatataga tcgcttattt ccataacatt gtccgtaaat
    27901 gacctttttt tgtcctctaa ggccaagcaa agacatcgcc ttttaagcta tattgtttct
    27961 atattaggct atgcttggaa ttataaaatg gatgatttaa aaaaatatag ttttttactg
    28021 gtctatttac ttatcccatt accctttgtc tctttatatt taggaggctg gtacagtttt
    28081 ctaccctttt ttattgtttt tgcttttatt ccattaattg atgcctttct tcatgattcg
    28141 gtaaatccaa caaaggagca ggaacaggag ctcctaaaag ataagttctt taaatatatt
    28201 acttgtgctt atgtgcctat acaaataacc atcattgctt atggagtcta tatagttagt
    28261 actcaaaatt tggtttggaa tgaatggtta ggatttacca tgtcactagg acttatctca
    28321 ggtggagtag gcatcaattt tgctcatgaa atgatgcata aaaacagtaa attgcaacaa
    28381 ctaatgagta aaattttatt agttacagtt tgctatggtc atttttttat cgagcatgtc
    28441 aaaggccatc atgttaaagt agcaacgcct gatgaccctg ccacagcacg attaggcgaa
    28501 aatttatatc aatttttacc aaaaacatta ttaggttcgt ttcaatctgc cttacttttg
    28561 gaagaaaagc gtttatcgca taaaaattat tgcatctgga gtatacataa taatttttgg
    28621 tggatcctta ctgttccatt catcatttgt atagcctgtt tcatttttgg aggattaatc
    28681 gctctgtcat ttttcttatt acaatcattc attgccatcc tgacattaga aattgttaat
    28741 tatattgagc actatggact ggaaaggaaa atgctgagta acggccaata tgaaaaggta
    28801 aatccaaatc attcatggaa tgctaatcat tggctaagta atcagctgct aattcattta
    28861 caacgccatt cagatcacca tgcccatggc gcacgaccct atcaaatttt acgtcatatg
    28921 gaaaacagcc ctcaacttcc ctcggggtac ctcggaatga ttatcatagc tttaattcca
    28981 ccactctggc attacatcat ggacaagaga gttgttgcct atcgaattca gttaagacaa
    29041 ttggaacaac tttaaaacgt tgttccttat tattatttca cagtaaaaga atattttatt
    29101 aacttttatt gcaatatggc ggtggaatca aaaactttta ttcgtttatt tagtaccgag
    29161 agacgatttg ccaaaatttt agccatacac atatgaaatt gaaccaagag taggggttta
    29221 tttttagaaa gctcctggat tgatttcaaa tcaatttcat aaacgatggt tttttcattg
    29281 gtttttaatg ttgctgtgcg cttcttatga gtgtacatgg caatttcacc aagtacatta
    29341 cctgaaccca caacaagtac acgtttatca ccgacaaatg ctgtaacttt tccccgatga
    29401 ataaagtaaa cactgtttga ctcttctcct tcatgacaaa tgatatcatc agggttataa
    29461 ggttttatag aagccttttc actaatccat gctaccagtt cagaggaaaa acctaaatag
    29521 agtaactgtt tctcaagtgt aacttcttca atatttatat tttcggtata tttttctata
    29581 acttgatctt cacaccactc taaagctgat tcttgatcag taaagacttt aagccaattg
    29641 gttttcgatt gtaataaatt ggttttcatt aattcatgat aaacaatagg ctttaatgaa
    29701 caaattgcaa aggaaatatt attcttttcc gcatggagct ttaagttttt cattaaaata
    29761 atacgggatg agtcgatatt gtagacaagc tcaaagtcta aaatgatata tcgagctttc
    29821 tgtatacttt ttattaactg aagtagtctg taaatagagc caaaaaaaag aaatccttgc
    29881 aacttcaaaa actgaatttc atctccctgt gtcgataaaa tggattgaga tatcggattt
    29941 ctctcaaagg aggagttata atctgagccg gaaaataaat acttaaccgg atttattttg
    30001 ctgtagcgaa aagcgaagat aaaaattgaa ataaccgttc ctatagcaac agccgtaatc
    30061 atattaaaga caatagcaat aaccaatatc agcaaaacga ttaaatattc gcttaaattt
    30121 aataagcgcc aagtattgat caaccattca gatagaaagc aaaaagcgat ataaaacaaa
    30181 taacagccga tagccatttt cgggaaacat tcaacgacaa cagcaccaaa gcacaaaata
    30241 agcaacatgg gaacgaaagc catcaccccg ataatacggg cgccgccata ctcttttgca
    30301 acagtggacg ctgacaagga caaatagcca accattcctc caagtaatcc actgaataca
    30361 ttgccaatac ctgctatacg aagttccttg tttaaatcca attgcttatt aactattaat
    30421 tcataactgc ttgcgtttaa taacaaggca ataaaactta ccagtataac caaccctatg
    30481 taattcaata gggtcattga gaatgggtaa tgaatcattt gccatatttc aggtttcatg
    30541 agcgaatcaa tatggttttg attgaaaggt cccaagacgt acccttcggt tggatcgacc
    30601 aaatactcca aattaaaaat ataaaaaaga gcatagaata gaattacaag agaaataaac
    30661 atcaattgaa gcgcgtatcg ataacctaat ttttctttaa aaatataaac aactattccc
    30721 gcaataaaac ctggcgccca caataatatt tcctctttat gaaaaaaaac aagccaattc
    30781 tgatgcagcg ctttaccagt taataatgca aatgttcctg ataaaactag ccagcctgtc
    30841 gctgccataa aaccacaaat cacaggatat ggcagataac ggataatatt tccaaaccta
    30901 aattttccaa cgaagtagaa tgataagccc gttaaacagg tagtgactcc aagcataaag
    30961 agaatcgtaa taaagatggc ttcattcgta gagcttgatg gcatcgattt aattatcact
    31021 aaagccatgg ctgaatacaa aattgccgct tcatcttgca actgagcgat tgcataagga
    31081 ggggaagaca tgaacaaata gctggcgctt ataataatta ctgaaattgc caatatatta
    31141 atgacaagtg gaatataaat ctctaatgca ccttgataaa taagtgaaga aaatgcaaca
    31201 atactaatca tattgtcaat acttatgacc aagcctgcta aaatagccaa aatcaattga
    31261 cttcgagtca tcctacctct cactcattca tttcaaaacc aaaggattat tttactaaac
    31321 aatgcaaaaa tccagttttt cacatttcta atggaattaa aatagcggcc tctattcatt
    31381 aatgattccg cataattttc tcctgccata gtaccgcttc attggcccag ataagctagg
    31441 catactcttg ttcgcgataa acttggtcta atccgattaa ctaacctgaa caatagctac
    31501 acaaaacttt ctctctattt aataattcct taataaaaat aaattaaaat ttaccagtca
    31561 attaattatt cacttaagaa gcgcattgtg cctgatgtaa gtgagtggag gactggtaaa
    31621 cttagtcaat cttaaatcaa aatagctgga tattattgag ataagtattt taagttaatc
    31681 aggcttcttt ccatcaaaat aaca
//
LOCUS       DAXLDT010000021        30557 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXLDT010000021 DAXLDT010000000
VERSION     DAXLDT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 30557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30557
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.021"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            210..866
                     /locus_tag="ACF2SP_000761"
     CDS             210..866
                     /locus_tag="ACF2SP_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFD2358563.1"
                     /translation="MQNIQHLIDYILHIDVYLNAFVSTYGFWTYLALFAVIFCETGLI
                     VTPFLPGDSLLFAAGSIAAQPGNPMNIMLLFVLLFMASILGNQVNFLVGRVIGPRVFS
                     AKRSWLLNPKHLHDTHAFYEKHGGKTIILARFIPIIRTFAPFIAGIGRMRSLHFTLFN
                     LISAILWIGSLLSLGYYLGSIPLIKENFSLVIYGVIAISILPPIIALISKKSTSSIKK
                     "
     gene            complement(930..3164)
                     /gene="lem21"
                     /locus_tag="ACF2SP_000762"
     CDS             complement(930..3164)
                     /gene="lem21"
                     /locus_tag="ACF2SP_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem21"
                     /protein_id="HFD2358564.1"
                     /translation="MAKTIKATGDGACLFNAVSIGLSVEILSGRLDSQLDTPGYQALL
                     DEFAKHHPQFNPKSWKTLKEWLAYYNDTRDIELILAPVLFNLNQKYQDHLDEEILNEL
                     TNLVWKNKANIENGQAWFQLQNTGDLGEALFPKLENLDLKKDRAPLLDKLREILKDYK
                     LELTRENVKQFLTEKAKELLSALKKKISSDPHAFQRGYSCDELKGMTDALAISLVENR
                     EEDITDNRIKIRLENKEEHWNVLCNEEDSERFLDSTPSRLKMTSLEAYRGDKQVSAPT
                     SEQLDLIEEPGVFLRERTIDNPGLGNCAFYAFAIGLVNIIQEEAKYNRRTMFDRWVGL
                     DRSISGQYDEILKLNLEDPDKELLDRLQSSLRIVTYQYQIRELRNVCVFRNGNYNRLT
                     GNSNFVNFASLYYGDPLDTDSRFNPFADSVPILIKMANTDRDSVHPGHENDVLVPLFL
                     DLLYGDATNPADITLETEPKSDSPIITAMNNITQDFFWGTHLDLNYLAEAFEVNLHVL
                     RNNSPIQEFVDIPERHTLTLTNSNNTHWTTQITTARATTGMLLGRNPHLSGAKRTGEQ
                     QRVNEEFLGKELANDFMIPPFDEISNPHHKKEVETNKPISKGFFHDRPVVSEEQRKLE
                     QLKRIVANATLAYTTYSESIWFCFFHYHGQTGRDRANNFCKTFSKITDYGQAKEALVH
                     YLNDSRNGNTHPHSYRTMLLHELQGNHDNKKDLQYLSRHFTSSLNKLASVLEVQLFAN
                     RYVP"
     gene            complement(3255..3953)
                     /locus_tag="ACF2SP_000763"
     CDS             complement(3255..3953)
                     /locus_tag="ACF2SP_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HFD2358565.1"
                     /translation="MNLGILLCDKVSEVFVADHGQYPEMFANLLRPADSTLEFTVFDA
                     EHGELPTDVHVVDAYLISGSRHGVNDNYPWIRKLEEFVCTLHASQKKLIGICFGHQLI
                     AKALGGKVIKSPKGWGVGMSQNQIYQFKEWMRPSLNCFNLLVSHQDQVIELPTGAEIL
                     AGSDFCPNYMMQIGSFFSVQGHPEFTKSYSRDLMVSREDSVNEIEFAKGMKSLELHED
                     DTIIAQWIVNFLKA"
     gene            complement(4083..5243)
                     /locus_tag="ACF2SP_000764"
     CDS             complement(4083..5243)
                     /locus_tag="ACF2SP_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFD2358566.1"
                     /translation="MKNQSLIVNWNYPTRILVGAGRINELAKACRELGMNAPLLVTDP
                     GLASSLMVSKVIQQAQASGLRTGLFCQIKTNPTGDNVMNGVCAFKAGQHDGVIAFGGG
                     SALDAGKAIALMVGQDRPLWDFEDVGDNWTRVNVSAMAPVLAIPTTAGTGSEVGRASV
                     ITDAEKQVKKIIFHPKMLPAMVILDPELTVGLPKLLTAATGMDALSHCLEAYCSNYYH
                     PMAEGIALEGIRLIKENLIQAYKDGSDLEARTHMLVASAMGATAFQRGLGAMHALAHP
                     LGAIYDIHHGRLNAVLMPYVLLANRSEIENKIERLANYLSIANGFDGFLDWIEQMRLE
                     LGIENTLSQLGIDHSQIDRLAKMATEDAAAASNTVLFTFEQYKELLSKAIGE"
     gene            complement(5373..6770)
                     /locus_tag="ACF2SP_000765"
     CDS             complement(5373..6770)
                     /locus_tag="ACF2SP_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFD2358567.1"
                     /translation="MIATLKTYSPVDNSLYVEREYANQEEIKTALSKAQVAKKLWSLS
                     SLAERQEYCLSAVNEIVACKVDIAEEICWQMGRPIRYAAGEINGFEERARYMIAQAES
                     SLASVELPEKEGFTRYIKREPLGLVLVIAPWNYPYLTAVNAIIPALMAGNVVILKHSA
                     QTPLVAERFDQAFKKAGLPMGVFQYLHLTHEDTEQLMRSPAVNSVAFTGSVAGGERVE
                     QTLAGRFIPVNLELGGKDPAYIRADADLEHAVETVIDGAFFNSGQSCCGIERIYVHKE
                     IYEQFVNKAVNLVKQYKLGRPDDPETTLGPLVRASSADFVREQIKEAIAQGAIAHINT
                     QDFVMDRPGTPYLAPQILTDVNHRMRIMTEETFGPVVGIMSVDSDEEAIERMNDSDYG
                     LTAAVFTQDIHRGIAIGERLQTGTFFINRCDYLDPGLAWTGVKKSGRGCTLSAIGYEY
                     LTRPKSFHIRTKIET"
     gene            complement(6808..8181)
                     /locus_tag="ACF2SP_000766"
     CDS             complement(6808..8181)
                     /locus_tag="ACF2SP_000766"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HFD2358568.1"
                     /translation="MLNPREVRTIEDAKRIVEERQLTHVKVGLFDIDGVMLGKYMRRN
                     KFFSALEHGFAFCDVILGWDSKDKLYDNVKYTGWHTGYPDASVRIIPSTCREILFEEN
                     QLLFMAEFSQAAEAICPRGILRRVINKSAGMGYDVYAALEYEFFVFDETPDSVRAKGF
                     RNLKPITPDNFGYSIIRNTVHAEFYHQILTMAEKMDFPIEGLHTETGPGVLEAAIAVD
                     DAEAAGDKAALFKTFMKILAQRNNKMATFMAKWSGDYPGQSGHIHLSLRDRDGGKSAF
                     YESGREYNMSKIQRHFLAGQQKLMPEFLAMLSPTVNSYSRLIPGYWAPTEATWGVENR
                     TTALRVIPGSDKSQRIEYRLGSADANPYLALAAAIGSGLYGIENELEPEAEVKGNSYE
                     QDHSPELALPRTLWESAQNLKNSSAAYELFGQEFVEHFAASREWEEREFRKHITDWEL
                     DRYFEII"
     gene            complement(8233..9021)
                     /locus_tag="ACF2SP_000767"
     CDS             complement(8233..9021)
                     /locus_tag="ACF2SP_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFD2358569.1"
                     /translation="MKSLLTLQTQFQEFIYSGQGSIKESIVPTKLVSTETRLNIYKDG
                     YRLRLIECLAANYPALYAYLGTEEFEKLSARYIDSYPSAYRSIRWYGDRLPELIKKYY
                     AKSCYYLYELAELEWKMTLAFDAADDTVLNVEEMAAVLPESWAEMGFVLHSSVHRLDF
                     CWNVFSLWQALVNNLEIPELKDSPATTGWVLWRGQNYIIQYYSLSEEEAWALDALIQG
                     SSFGVICEGLCQWMPAEEVGMKAASYLKNWIQNGLLSQCRIRRN"
     gene            complement(9018..9881)
                     /locus_tag="ACF2SP_000768"
     CDS             complement(9018..9881)
                     /locus_tag="ACF2SP_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFD2358570.1"
                     /translation="MEVSPNKSDQKLPYLGFGLGLRPNYYEEILTSKPDLDWFEILTE
                     NYLIPGGKPLYYLDKIREYYSIVMHGVSLSLGSTDPLNWDYLKQVQELASRIEPVWIS
                     DHLCWTGIHGLNTHDLLPVPYTTEAIQHIVSRIQEIQDFLKRPFLIENVSSYLTYKQS
                     EMSEWDFILEIVKQSGCYLLLDVNNVYVSSFNHNFDPMAYINSMPPGRVAQIHLAGHT
                     NHGDYIIDTHNAPVIEQVWDLYEATIQRLGPVSTMIERDDNMPDFSELLSEINHAKRL
                     AIQAEKEKVAV"
     gene            complement(9957..10208)
                     /locus_tag="ACF2SP_000769"
     CDS             complement(9957..10208)
                     /locus_tag="ACF2SP_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358571.1"
                     /translation="MNKLQLTGAALAVGAASMFALAPAFAVDATTTTIHCKGVNACKG
                     QGSCKTADNACKGQNSCKGKGILEMSKEDCEKQGGTVAE"
     gene            complement(10638..11996)
                     /locus_tag="ACF2SP_000770"
     CDS             complement(10638..11996)
                     /locus_tag="ACF2SP_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD2358572.1"
                     /translation="MKLSFLGATGTVTGSKYLVESNNKKILVDCGLFQGLKELRLRNW
                     SPLPVSPKSIDAVVLTHAHIDHSGYLPLLVKQGFKGRIYATGATTDLCSILLPDSGFL
                     HEEDARRANKYGYSKHHPALPLYTQEDANNTLKYFESVEYDTPYPLMEDCEISWHRAG
                     HILGSAFIQFQTKQIKLLFSGDIGRLNDPVMKPSELMKSTDYLVIESTYGNRLHDKTD
                     PLAFIANIVNTTCKRGGTVLIPAFAVGRAQTMLYYLYILKQKKLIPDVPVYLDSPMAI
                     DTTELLLKHHNEHRLSAEECRAVCQIATYVHTPNESMAVDWGRESKIIISASGMMTGG
                     RVLHHLKVYAPDPASTILLTGFQAAGTRGEKMLNHEPSVKIHGDDYPVRAQVAMLTNA
                     SAHADYEEILVWLKHFEKAPKEVFITHGEPQAALGLKGHIEAELGWNCKIPNYLEKVN
                     LD"
     gene            complement(12119..12769)
                     /locus_tag="ACF2SP_000771"
     CDS             complement(12119..12769)
                     /locus_tag="ACF2SP_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358573.1"
                     /translation="MNYFIGLCFGLLNAVICYAEPNGTSLPKELIKVNKENNPKCVEF
                     VTYKGELYCSVVPLADSSGDSQVIHYENQIVRFDDRPWKIAWGKKTDKVVTIEYIPVG
                     DDINNWKELITTQFMPGLINMTPAQFGNQFFHNLNKSGVKYIVHVIEEQPNLLIFEFK
                     IQEPANLQQDEILKITKGKDGIYVLHYAIKKSDMTKENRDKWVKNLKSSFIKASTP"
     gene            12886..13176
                     /locus_tag="ACF2SP_000772"
     CDS             12886..13176
                     /locus_tag="ACF2SP_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage holin"
                     /protein_id="HFD2358574.1"
                     /translation="MNGCKISPVGLGLSLGILWGLSLLVMGLIAYFYAYGRPFVEAVA
                     TLYLGYEPSILGSFIGGVIGFIDGFVTGFLIAWLYNCFSCCSCCRGKENKKE"
     gene            complement(13267..13578)
                     /locus_tag="ACF2SP_000773"
     CDS             complement(13267..13578)
                     /locus_tag="ACF2SP_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFD2358575.1"
                     /translation="MLKCLSSVIIVFLILFTAGCQTNTVSNMFIPSNPSGMTLAQSVK
                     DSLMESNDPVINQIHVESNQNVVILSGYVKKIRQSDIAEQIARQVQGVQSVENRIIVR
                     P"
     gene            13795..15582
                     /locus_tag="ACF2SP_000774"
     CDS             13795..15582
                     /locus_tag="ACF2SP_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD2358576.1"
                     /translation="MKKTTEKPINKTKEMPASSKKEILNPLEQTPSLEQSDPIFRFID
                     KLYQANLGKLTAGISPAALGTAYYSWFAQLLQSPGSMLRLAYYPLLHANDYLSNLFKY
                     DKPRDGKDVRFHTENWSYYPWRLWAEQFLQFEDWCLQASSKVPGIPLHVKRTVTFSTR
                     QILDALSPSNFVLTNPDLLQETIRSNGQNLIRGTELAFQDFVEKITGSPPAGVENFIP
                     GKQVAVTKGKVVYSNHLIELIQYTPQTEKVYKEPILILPAWIMKYYILDLLPENSLVN
                     WLVRQGHTVFIVSWRNPTKEDRNLGLDDYYKLGAMDAINAVSNAIPHTKIHLMGYCLG
                     GTLALLTAAAMAHDHDNRLKTLSLLAAQGDFIDAGELLLFITKSEVSFLKSMMWEQGY
                     LDTKQMSGTFQMLRSYDLIWSKMVQDYMHGTQRGMIPLLAWNADATRMPYKMHSEYLE
                     KLFLNNDFAEGRFILDGKPVVGENIRIPAFVVSTEKDHVAPWKSVYKTHLLINSDITF
                     VLTNGGHNAGIVSEPGHEGRYYRIRERKMDSTYLDPTNWLKKAELREGSWWIAWHDWL
                     VNHSSQKQVSAPKLDKNLPNAPGKYVLQK"
     gene            15594..17000
                     /locus_tag="ACF2SP_000775"
     CDS             15594..17000
                     /locus_tag="ACF2SP_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFD2358577.1"
                     /translation="MNNEFLENVTYEELTIGRKASLNRTLTQDDINLFAAMSGDINPS
                     HVDPQFAKSDVFHGVVGHGMWSGALISTLLGTALPGPGTIYLEQDIQFITPVRIGDNL
                     TITIVVRDKHPGKSIVHFDCMGVNQKGETVIKGLAKVIAPTKKMKVPRTVLPLVQIHH
                     NDHFNKIIEACSNLPSIKTAVVHPVTANVLEAVYDSVKAGLITPVLIGPMAKIKCAAS
                     EAKIDLSNWEMIDTEHSDAAAFKAVELAASGKVDSIMKGALHTDELMSAVVPATSGLR
                     TKYRISHAYVMDVPTYHKPLIITDAAINIAPNAADKADICQNAINLWRILYGEEIKPK
                     VAILAAVEMVNPKMQATVDAAVLCKMADRAQIVDGILDGPLAFDNAINKQASKEKNII
                     SPVAGDADILLVPDIESGNMLAKQLTFLGHADAAGIVLGARVPIILASRADSLRTRLL
                     SCAVATKIAAARKAGKIK"
     gene            16997..18115
                     /locus_tag="ACF2SP_000776"
     CDS             16997..18115
                     /locus_tag="ACF2SP_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFD2358578.1"
                     /translation="MRHSALLIINSGSSSVKFQVFSNTENLDLLAKGKIINIGGEPSF
                     TAVNETSKHQRTLITLEKGMTYEQAIHLILDWISQQQQNWEIHAVAHRVVHGGEYFTQ
                     SVLVTPQIISQLETLNSLAPLHQPHNLAAIKIIKKIKPELPQIACFDTAFHAQHTPLF
                     TTCALPEYLRQQGIRRYGFHGLSYEWISLVLKKDYHDLANNRIIIAHLGNGASLCAMD
                     KGVSIDTTMSLTALDGLPMGTRCGSLDPGAVIYMMRELNLPPDKLEEILYNDSGLLGL
                     SGKTNNVLELQSSPDKNAQFALEYFCLKTAQFIGMMTISLGGVDGIVFTGGIGENSES
                     IRANILKRIECLHPFSVIVIPANEEKMMAMHTLTALRT"
     gene            18154..19044
                     /locus_tag="ACF2SP_000777"
     CDS             18154..19044
                     /locus_tag="ACF2SP_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HFD2358579.1"
                     /translation="MDKDYYKIMGVSQDASDKDIKMAYRKLARKYHPDISKEPDAEER
                     FKEMAEAYEVLKDPKKRSEYDQYLKYKEFNPQSDGFTGRRTQEQPFQEFHFDSDFFET
                     LFGHSRYQQQPMTGQNYHGKITISLEEAYHGAVKNLQVPVEQGTETKIQTLKVKIPPG
                     VKSGQQIRLAGQGGSGSGGGARGDLYLTVEVMKHPTFDVMENDIYLTLPITPWEAALG
                     ATVVIPTLAGKIDLKIPPGSQGGQKLRIKNRGLPGSPPGNQYVLLKIITPPAQTEEAK
                     ALYKKMAEVMPYNPRKTMGV"
     gene            19047..19361
                     /locus_tag="ACF2SP_000778"
     CDS             19047..19361
                     /locus_tag="ACF2SP_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFD2358580.1"
                     /translation="MSKDNLLIGMLIEETTTISFIEVCQKYHIPEELFIEMIEQGLFP
                     DQPSDPEKIALGQKELRRLESAFRLHRDLGINLPGVALALDLLEKIDQMHHELEILRK
                     HF"
     gene            complement(19358..19921)
                     /locus_tag="ACF2SP_000779"
     CDS             complement(19358..19921)
                     /locus_tag="ACF2SP_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFD2358581.1"
                     /translation="MQKICRFIFKLIGWKIVGELPNEKKYIVIVAPHTSNWDFVICLC
                     ARFVVGVKINFLAKHQLFFFPLSYFLRAVGGTPVDRSKKGNRVEKVVELFRQRDELKL
                     GLAPEGTRSPVTRWKEGFYHIARQAGIPIVMVGPDYFTKEIRIHEPFWTTGDIKKDFQ
                     YIIEFFRTIKGYRPKEIPDYNPKDSDA"
     gene            20039..20590
                     /locus_tag="ACF2SP_000780"
     CDS             20039..20590
                     /locus_tag="ACF2SP_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFD2358582.1"
                     /translation="MLYHDPLISEHTMKWVRTFIINYTICPFAKGPVNKGTLRIITSD
                     TLKKHQALKDLITEIQFLEANPAVETTLLIFARAFKDFFSYLDFISFAEQLIQNLNYE
                     GIYQLATFHPDYYFADTSPDDVANYTNRSPYPMIHLLREDSLDKAIAAYGDTHIIPEK
                     NIATMNELGLEKMKQLLASISIP"
     gene            complement(20623..21861)
                     /locus_tag="ACF2SP_000781"
     CDS             complement(20623..21861)
                     /locus_tag="ACF2SP_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFD2358583.1"
                     /translation="MSSILFKNANVILGESTEIQKNFDVMVQNDLIAQVSQTPLQPLE
                     GMRVIDVKGKTLMPGLIDAHAHVTGLTLSPKNIFYSEAEIFLAAANYLKNSLFYGFTT
                     LREAGGADFRIAQLLDNKSIPGPRLFYSGKALTQTGGGADFRKPNEQIDPCGHVSSFS
                     TMSVIADGVDEVRKAAREELRKGATQLKVFASGGVVFPSLSNPTLYEYSEEELSTIVE
                     EAHARNTYVMAHAYSDESVRKCIKSGVRSIEHANFVSEPTVELMSESGVVYDPTFISL
                     AQRIESAEQNRLSKAIVANLKNTIEKGKKVYEYALKYKIPIAFGTDLWGPEAQRDQLR
                     EFEMRKELDSAANIIRSATVVNAELLMQKGKLGVISEGAYADLLVVDGNPLVNLNVLL
                     RPDENLKLIMKDGVIYKNEL"
     gene            22062..22622
                     /locus_tag="ACF2SP_000782"
     CDS             22062..22622
                     /locus_tag="ACF2SP_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358584.1"
                     /translation="MNTQDPALSISNPRLLSAIYFGLLSVVGTILINAFLTSIGIEEI
                     IPVYQSVILGMVVASCTGAIFGEAIVHCKKPYKRKTFWLGFSMVIASLPVFDLGLVFL
                     MSEANAKLFEVTDIGSMVLFYLVVLAYSYVLFGILLAVASGLAAMYLRGQLVYDILHT
                     QTRRQQKQKHALAKNKSEGHAHKVHR"
     gene            22570..25146
                     /locus_tag="ACF2SP_000783"
     CDS             22570..25146
                     /locus_tag="ACF2SP_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFD2358585.1"
                     /translation="MHWQKINQKDMPIKFTDELKSDELYNLLSQGAVVITPNNRLSGT
                     ILQNYFTYCSRKTITKPICLPFNTFLVNSFEQLKFQKPDLEHPILLNSAQCQHLWRKL
                     IKSHPSIIYTEGLLKTIMQAWEYCEQWQLTPEAPAFHYTPQTQQFQQWWQSFNKILKE
                     ISAIHQHQLIRYLLDSNYQGLVKTVVWMCFDDFNPQQLSLQNYLNAQGVIQYGYDLKE
                     NQETPEVFSAKDKKTEYQELMTWLEKKIQQGKKQIGVVVPNLEQESSVLQRVLRDCFE
                     PNLFNISLGKPLSAFHLISHALIWLSLDHNLLNNHQAALLLQSPYIGKAKDEFLQRSD
                     YLQESKLLQDQSFPTKLFIEDVAYHTPKLAELLKNMNFYPEKTTLDEWIELFQERLNG
                     IGFPGDYVLNSEDYQCFNRFTMIFDEFRQLSLISSHLTKKEAFETLKQLIDNTIFQPQ
                     KSNSPIQILGLLEASGCEFDSLWVMGLTDRCLPQQTRLSPFIPFELQRELGMPHSMPA
                     RELQYAKQILQRLQRGSMETVFSYPQFDDDTPNLPCSLITHYPAYLSRSADNKTNVQS
                     CLVPLEENYEVPVKSDESISGGTSLLSNQAKCPFKALAQHRLKANPVLKTTDGMDDKE
                     KGQLIHKILELLWQRLENQEHLIKLKPAELERFIDEAIQGALNPIRQRHSQAFPDLIA
                     NVEYMRLKRIILSYLEWEKQRPPFKITGLEENHSINLAGLDFKVRVDRLDEVGNKQWV
                     IDYKSTLPGGKPWNEERPMEPQLLLYALLNEQINTLLLIQLKTGKIKLSGFSEEKWET
                     KEINTLKEDENWEDYRQRWLEQLTHLAEEIKSGYCPPRPQNLTICQRCDFQNLCRFQA
                     KQ"
     gene            25174..26016
                     /locus_tag="ACF2SP_000784"
     CDS             25174..26016
                     /locus_tag="ACF2SP_000784"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2358586.1"
                     /translation="MHHLILGYGYCGYYLAQELLRHHQQVTVVSRQLKKELELSKLQH
                     ISHDLESPFHWSEPDSIIYYLIPPPSQGTCDTFLQQFLNQSSLNAKKIIYFGSSGVYG
                     NHQGAWVDELSACIIDSPRQQRRLDAEQQWLAYCQQHNKELSLLRIAGIYGPNRIPAD
                     AAKSKIPIIKPTEAPFTNHIFVKDLSLIAYLLGQKQTTSAVYNVADGNPTHMGELQQT
                     VANALGIESASYESFEQAWEKASPMKREFMRASKRLKIDLLKTALGEDIQLTCLYDAV
                     KQSL"
     gene            26168..26818
                     /locus_tag="ACF2SP_000785"
     CDS             26168..26818
                     /locus_tag="ACF2SP_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2271 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358587.1"
                     /translation="MPSYNNARLLEKRLQNCDTQLNQFFNGQELSIKFLQQLNQIKYF
                     YNSAFSKTENEDDGLEVVEEYENFISLVSQVKSGQINADKAFETIKDTTESRQADVII
                     ANFFKVCELMFWATAAFFSYLACVSVGIPLTFCDPLLGIAVSLSTFLMTISTASKFLD
                     CFDEFQSLDKINEHDKNQKDTVRFFSKTSNSTSSVRNKEAEVEESSQRLYPNLQTV"
     gene            complement(26992..27480)
                     /locus_tag="ACF2SP_000786"
     CDS             complement(26992..27480)
                     /locus_tag="ACF2SP_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 family protein"
                     /protein_id="HFD2358588.1"
                     /translation="MIIRFSKVLLVVAVSFFCLLTAFGNITDYSANFPAVVKVLTMSD
                     IFPNSTITYRAITSPALHYVAFIIIVILELLTAIFCGVGAWKLFKARNESASVFNHSK
                     NWAIGGLTLGFVTWQVIFMSIGGEWFGMWMSPMLNSALTTAFHIFITILAVMIYLVIK
                     DE"
     gene            complement(27625..28938)
                     /locus_tag="ACF2SP_000787"
     CDS             complement(27625..28938)
                     /locus_tag="ACF2SP_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFD2358589.1"
                     /translation="MKILFLLLLIFLSVIPAQAKPVELVFWHAMAGHLGDEVRLLADD
                     FNKSQNQYRVKPIYKGNYTETLTNFAAAFRARQAPSIVQIFEVGTSIMLAPPGVIKPV
                     DLLMSEQGISLPKDDFIQSVREFYSRDGQLMAMPFNLSAPVLYYNADILAKVGYGKYN
                     FPQTWSAMEIMAEKIKKAGYDCTYTTAYPGWVLFESFLAIHGLPITQGNPARAAFHTP
                     QLMAHFQRLKRWHDLHYFRYGGRVDDATILFTSSVCPLFSQSSGAYNSLSALVPFHLG
                     VATMPLDTGASPIRHANVAGGAALWAVGGQTEEQYRGIARFFAFIAKPEVQKRWHEHT
                     GYLPLGLKGIYANIVQSSQHPALLLARTDLEDNLSEKPYKHMGPQNQIRGINDEVLEA
                     MFADLMSPEEALNEATIRANHLLLRFARNTQRERVVDDKVGKGLG"
     gene            complement(28935..29654)
                     /gene="ugpQ"
                     /locus_tag="ACF2SP_000788"
     CDS             complement(28935..29654)
                     /gene="ugpQ"
                     /locus_tag="ACF2SP_000788"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFD2358590.1"
                     /translation="MILEKIIGHRGASGYAPENTMASFNKALSLGCRFIEFDVMCSLD
                     GEPFIIHDDNLKRTTNGKGEVGLVEADYLRSLDAGSWYSKQYKGEKVPHFTDVLKWLS
                     FSDVQANIEIKPYPGTVEQTTVSVLSYIHRYWPQNKELPLVSCFEWDALVLCRSIAPE
                     MPLGLLLHEWDKDWLQKAKQLECYSIHFNRKILTADRVKAVKDQGYVVCAYTVNRKRL
                     AKKLFDWGVDALFSDYPDLLK"
     gene            complement(29659..30354)
                     /locus_tag="ACF2SP_000789"
     CDS             complement(29659..30354)
                     /locus_tag="ACF2SP_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358591.1"
                     /translation="MRKIAWILGLMVLTPFVWADNETPDLVLDKIFFQVSAKKWVTTQ
                     TALLNVSINATLSNADLVKARADIMDSLNKIAKGEWHLVQFDRSQDSSGLEKLYVQAQ
                     ARVDQSALTNIYQNAKSVSKPGAKYEISGVEFKPSLEETQVVRAKIREQLYQQVNDEI
                     DRMNKAYPKQNYSVSNLVFFEGDNIPQQPRAYQAKEMNALVMAAAPAPLTVSNELILT
                     AMVEAASNRKQGD"
BASE COUNT         9442 a         6562 c         5498 g         9055 t
ORIGIN      
        1 gatacacaga gagccgttca ggctgaggag atgcataagc atcgtcacga agacttggta
       61 tgaaacatta aatctcatac aaggcttcga gacgacatat aaataccccc tcagcccgag
      121 cggtttgaga taacaactga ttttatccat aactcacgtt agtatagata catggaattc
      181 acgtcataca tttaatccat agagacacta tgcaaaacat acagcacctg attgactaca
      241 ttcttcatat cgatgtttat ttaaatgcct ttgtatcaac atatgggttt tggacttatc
      301 ttgcgttatt tgctgtcatc ttttgcgaga caggccttat cgtcactcct ttcttaccag
      361 gagactccct gttatttgct gcaggaagta tagcagctca accaggaaac cctatgaata
      421 tcatgctgtt gtttgttttg cttttcatgg cttcgatatt agggaatcaa gtgaatttcc
      481 ttgttggacg tgtaataggc cctcgtgtct ttagcgccaa gcgctcctgg ttactgaatc
      541 ccaagcatct gcatgatacc catgcttttt atgagaagca tggcggcaaa acaataatac
      601 tggcgcgttt tattcccata atacgcacct ttgctccatt tattgcaggt attggcagga
      661 tgcgatcatt gcatttcacg cttttcaatc tgatcagcgc catactttgg attggaagtc
      721 ttcttagcct gggatattat ttaggctcaa tcccgttaat aaaagagaat ttctcattgg
      781 ttatctatgg ggtcatagcg atttcaattt taccgcccat catcgcttta attagtaaaa
      841 aatcgacgtc ctcaattaaa aaataatata ctctagggac ttatgcgagt gcaaagggct
      901 ctaaggatgc cttttacacc acgctcttac tagggaacat atctgttggc aaacaattgc
      961 acttctaaaa cagatgctag tttatttaaa gaagaagtaa aatgcctgga taaatattgc
     1021 aaatcttttt tattgtcatg attcccttgt aattcatgca gtaacattgt tctataggag
     1081 tgtggatgtg tatttccatt cctgctgtca tttaaataat gaaccaaagc ttctttggct
     1141 tggccataat ctgttatttt agaaaatgtt ttgcagaagt tattagcccg atcacggcct
     1201 gtctgaccat gataatgaaa gaagcaaaac cagatgcttt cactgtaggt ggtgtaagcg
     1261 agcgtagcat tggcaacaat ccgttttaat tgctccaatt ttctctgctc ttcactaact
     1321 accggtctgt catggaaaaa ccctttactg attggtttat tggtctctac ctctttctta
     1381 tgatgggggt tagatatttc atcaaaagga ggtatcataa aatcgttggc taattctttt
     1441 ccaagaaact cctcgttaac tcgctgttgc tccccggttc ttttagctcc actcaggtga
     1501 gggtttcgcc ccaaaagcat tcctgtagtg gctctagccg ttgtaatttg ggtagtccaa
     1561 tgcgtattat tgctattagt taaagtaaga gtgtgtcgct cgggaatatc gacaaactct
     1621 tgaattggac tattattcct tagcacatga agattcacct caaacgcttc ggctaaataa
     1681 tttaaatcca aatgggtgcc ccagaaaaaa tcctgagtga tgttattcat cgcagtaatg
     1741 ataggtgaat cactctttgg ttctgtttcc agggtgatat cagctggatt cgtagcgtca
     1801 ccatataata aatctaaaaa gagaggaacc aatacatcgt tttcatgtcc tggatgaaca
     1861 ctgtctcgat cagtgtttgc cattttaatc aatataggaa cagaatcggc aaaaggattg
     1921 aaccgagaat ctgtatccag aggatcgcca taatataagg atgcaaaatt aacaaaattg
     1981 ctgtttccag ttaatcgatt ataatttcca tttctgaata cacagacatt tcttaattct
     2041 cgtatttgat attggtaggt cactattctt aaggagcttt gcaacctgtc cagtaattct
     2101 ttatcgggat cttcaagatt aagtttcaaa atctcatcgt attgacctga aattgacctg
     2161 tcgagaccta cccatcgatc aaacatggtt cttctattat actttgcctc ttcctgaatt
     2221 atattcacca aacctatcgc aaaggcataa aaagcgcaat tgccaaggcc agggttatca
     2281 atggttcttt ctcttaaaaa aacgccaggc tcttcaatca aatccaactg ctcagatgtt
     2341 ggtgcactaa cttgtttatc acctcgataa gcctccaagg aagtcatttt caaacgtgag
     2401 ggtgtcgaat ccagaaaacg ctcgctatcc tcttcattac acaatacatt ccaatgctct
     2461 tccttgttct ctaatctgat cttgattcgg ttgtccgtaa tgtcctcttc tctattctca
     2521 accagactga tcgctaaggc atctgtcata ccctttaact catcacagct ataacccctt
     2581 tgaaaggcat ggggatctga actgatcttt ttctttaaag cggacaataa ttctttagcc
     2641 ttttcggtca gaaattgttt tacattttca cgtgtcagtt caagtttgta gtctttaaga
     2701 atttctcgta atttatctaa taaaggagcc ctgtcttttt tcaaatccag attttccaat
     2761 ttgggaaaaa gtgcctcgcc caaatcccct gtgttttgca attgaaacca ggcttggcca
     2821 ttttcaatat tggccttatt tttccataca agattggtta attcatttag aatttcttca
     2881 tccagatgat cttgatattt ctgattcaaa ttaaataata cgggtgccaa aatcagctca
     2941 atgtctctgg tatcgttgta ataggctagc cactctttta aagttttcca gcttttgggg
     3001 ttaaattgcg ggtgatgttt ggcaaattca tccagcaagg cctgataacc gggcgtatcc
     3061 agctgcgaat ccagtcttcc gctcaagatc tcgacactca gtccgattga cactgcatta
     3121 aatagacaag cgccatctcc agtagcctta attgtcttcg ccatacatac tcccgatagt
     3181 tctggtcagt gaataaaatt aatgttcaca tattgtactc taagggggta taataatcaa
     3241 ttaaaaacag ctcatcaggc ttttaaaaaa ttaactatcc actgggcaat aattgtgtca
     3301 tcctcatgta actcaagaga tttcatccct tttgcaaatt caatttcatt gaccgaatcc
     3361 tcgcgagaca ccatcaaatc acgagaataa gatttagtaa actcaggatg cccctgcaca
     3421 ctaaagaaag aaccaatttg catcatataa tttggacaaa aatcactccc tgccaatatc
     3481 tccgcaccgg ttggtaactc aatcacctga tcttgatggc taactagcaa gttgaagcaa
     3541 tttagagatg gtctcatcca ttctttgaat tggtaaattt gattttgtga catacctacc
     3601 ccccaaccct tgggagattt aatcactttt ccacctaagg ctttcgctat taattgatgc
     3661 ccaaagcaaa taccaattaa ttttttttgg gaagcatgca aagtacaaac aaactcttca
     3721 agctttctta tccagggata attatcattc accccatgtc gacttcctga tatcagataa
     3781 gcgtctacga catggacatc tgttggcagc tcgccatgct ccgcatcaaa tacagtaaat
     3841 tcaagagtag aatccgccgg acgcaataga ttggcaaaca tctcagggta ttgaccatga
     3901 tccgcaacaa atacttcact taccttatca caaagcaaaa taccaagatt catttaattc
     3961 acctaattaa ttatcgcaaa atttttattg tctggattag acttaatttc aatatacaag
     4021 tcctttccat ctgatttata cctggcaaac aacccgtttc tgcatctttt cctttacaac
     4081 gattactcac caatagcttt gctcaatagt tctttgtatt gctcgaatgt aaaaagaaca
     4141 gtattggaag ctgctgccgc atcctcagtg gccatttttg ccagtctgtc tatctgcgaa
     4201 tgatcaatac ctaactgact tagtgtattt tcgattccta actctaatct catctgctct
     4261 atccaatcca aaaaaccatc aaatccattt gctattgaca aataatttgc cagacgctct
     4321 atcttgtttt caatttcact acgattagcc aataaaacat agggcatcaa aaccgcatta
     4381 agccgcccgt gatggatatc ataaattgca cctaatggat gagccaaggc atgcatcgct
     4441 ccaagaccac gctgaaaggc agtagctccc attgctgaag caactagcat atgggttctt
     4501 gcctccaggt cactaccatc cttgtaagct tgaattaaat tctccttgat taatcttatt
     4561 ccttctaacg caataccttc tgccatagga tgataataat tggaacaata agcttcaaga
     4621 caatgggaaa gagcatccat acctgtggct gcagtgagta atttgggaag cccgactgtt
     4681 aactctggat ccagaataac catggcaggc aacattttag gatgaaaaat aattttttta
     4741 acctgctttt cagcatcggt aattacagaa gctcgcccga cctctgaacc agttcctgct
     4801 gttgttggaa tggcaagaac tggggccatg gctgaaacat tcactcgagt ccagttatca
     4861 cccacatcct cgaaatccca taaaggtcga tcctggccaa ccattaaagc gatggctttg
     4921 ccggcatcca aagccgagcc acctccgaaa gcaatcacgc catcatgctg gccagcttta
     4981 aaagcacaga ctccattcat cacattgtcg ccggtaggat ttgttttaat ttggcaaaat
     5041 aaccccgttc gcaaaccgga tgcctgagcc tgttgtatga ctttactcac cattaacgac
     5101 gaagccaatc ccgggtctgt gactaacaga ggagcattca tgcctaactc acgacatgct
     5161 ttcgctagtt cattgattcg cccagcccca accaggattc gtgtcgggta attccaattg
     5221 acaattaatg attggttttt catgtatgat cttcctttga ttcgaaaaag accattgtaa
     5281 ctgatagcaa gtacctttcc gcaggaaaga ttaggtcttt tttataattt tattttgagt
     5341 tttaagacgt aagtaatcgt aggtatcagg ctttaagtct caattttagt tctgatatgg
     5401 aatgatttgg gccgcgttaa atactcatag cctatggcgg ataaagtgca acccctgcct
     5461 gattttttta cacctgtcca agccaaaccg ggatcaaggt agtcgcaacg attgataaaa
     5521 aaagtccctg tttgtaatcg ttcaccaatc gctattcctc gatgaatatc ctgagtaaat
     5581 acagcagcgg tcaaaccata gtcactatca ttcatccttt cgattgcctc ttcatcacta
     5641 tcaaccgaca tgatccctac gactgggcca aaagtttctt ctgtcataat tcgcatgcga
     5701 tgattaacat cggttaaaat ttgcggtgcc agatagggtg tgccaggtct atccattaca
     5761 aaatcctggg tatttatatg tgctattgcc ccttgggcta tcgcttcttt gatttgctct
     5821 ctgacaaagt cggctgagga agcacgtacc agtggaccca gagtggtttc tggatcatca
     5881 ggccgtccca atttatactg tttaaccaaa ttaactgctt tattaacaaa ttgttcgtat
     5941 atctccttat gaacataaat cctttctata ccacaacagg attgacctga attaaaaaac
     6001 gcaccatcta tgactgtttc tactgcatgt tccaaatcag cgtctgctct gatataagcc
     6061 ggatcttttc cacctagctc caaattcaca ggaatgaaac gtcctgccaa tgtctgttca
     6121 actctttcac caccagcaac tgaacctgtg aaagctacgg aattaactgc tggcgatctc
     6181 atgagttgtt ctgtatcttc atgtgtcaaa tgcaaatatt gaaaaacacc catgggtaag
     6241 cctgcttttt tgaatgcctg atcgaaacgc tcagccacta acggagtttg cgcagaatgc
     6301 ttcaaaatca caacattacc cgccataagt gcaggaataa tcgcattgac tgctgtgaga
     6361 taggggtaat tccatggcgc tataaccaac acaagcccta acggctctcg tttgatgtat
     6421 cttgtaaatc cttctttttc aggtaattcc acagaagcca gtgatgattc ggcctgtgca
     6481 atcatatatc ttgctctctc ttcaaaccca ttaatttcac cagctgcgta tcgaattggt
     6541 ctacccattt gccaacaaat ttcttctgca atgtctactt tgcaagcgac tatttcattg
     6601 acagcagaca aacaatattc ctggcgttcg gccagagaag acaaagacca cagttttttt
     6661 gctacttgtg ctttgcttaa agcagttttt atctcttctt gattcgcata ctctctctca
     6721 acataaagag agttatcgac aggagaatag gtttttaagg tagcaatcat ttttaatcca
     6781 taagttttat aaaatctgtc gtttcaatta aataatttca aaatacctgt cgagttccca
     6841 atcagtaata tgttttctaa attctctctc ttcccattct cttgaagcag caaaatgctc
     6901 aacaaattct tgaccaaata attcataagc agcagaagag ttttttaaat tttgtgccga
     6961 ttcccataat gtgcgaggca acgccaattc cggactatga tcttgctcgt aggaattccc
     7021 ttttacctca gcctcaggct ccaactcatt ctcaatgcca tataaaccgg accctatggc
     7081 agcagccaac gcgagatatg ggtttgcatc tgcagaaccc agccgatatt caatacgttg
     7141 tgacttatca ctgccgggaa tcactcgtaa agcagtggtg cggttctcga caccccatgt
     7201 tgcctctgtt ggagcccaat aaccgggaat tagtctggaa tagctattca cagtcgggga
     7261 taacatggct aaaaattcag gcatcaattt ttgctgcccg gctaaaaaat ggcgttgaat
     7321 tttgctcata ttatattctc tgcctgattc ataaaaagca gacttgccgc catctctatc
     7381 tcgcaaagac agatgtatat gcccactttg cccagggtaa tcacctgacc atttagccat
     7441 aaaagttgcc attttattat tgcgttgcgc gagaattttc ataaatgttt taaacaaagc
     7501 cgctttatct cctgctgcct cggcatcatc aacagctatc gctgcttcca gaactcccgg
     7561 gcctgtttca gtatgtaaac cttcaatggg gaaatccatt ttttccgcca tagtgagaat
     7621 ttgatgataa aattcagcat gtaccgtatt acgaataatt gagtagccaa aattatcagg
     7681 agtaataggc tttagattac gaaatccttt tgcacgcacg ctgtcaggtg tttcatcaaa
     7741 tacaaaaaat tcatattcca atgcggcata aacatcatac cccatacccg cagatttatt
     7801 gattacacga cgcaagatcc cgcgaggaca aatagcttct gctgcttgtg aaaattcagc
     7861 cataaacaac agttgatttt cttcaaataa tatttcacga caggttgaag gaataattcg
     7921 cactgaggcg tcaggataac ccgtatgcca gccagtatat ttcacattat cataaagctt
     7981 atctttagaa tcccatccta aaatgacatc acaaaaagca aaaccatgct ccaaagccga
     8041 aaaaaacttg ttacgtctca tgtacttgcc cagcatcaca ccatcgatat caaaaagacc
     8101 aaccttaaca tgagttaatt gtctttcttc cacgatgcgt tttgcatctt ctatggttcg
     8161 gacctctctt gggtttagca tggcatatcc ttatgtcgtt ctgtcaatgt aagcttcttt
     8221 acctcccaat gtctaattgc ggcgaatacg acactgagat aataacccat tttgaatcca
     8281 gttttttaaa taagacgctg ctttcattcc gacctcttca gcaggcatcc actggcataa
     8341 tccctcacaa atcaccccaa aagaggagcc ctgaatcaga gcgtctaaag cccaagcctc
     8401 ttcttccgac aagctgtaat attgaatgat ataattttga ccccgccata acacccagcc
     8461 agtagtagca gggctatctt tcaactctgg aatctcaaga ttattgacca aagcttgcca
     8521 caatgaaaaa acattccaac aaaaatcaag cctatggact gatgaatgaa gtacgaaccc
     8581 catctcagcc caggattcgg gcagcactgc tgccatctct tctacattaa gcactgtatc
     8641 atctgctgca tcgaaggcta aggtcatttt ccattccagc tcagccagtt catacaaata
     8701 atagcaagac ttcgcatagt acttttttat caattcaggc aatctatcac cataccatcg
     8761 gattgaacgg taagcagatg ggtaagaatc aatataacga gcacttaact tttcaaattc
     8821 ctcagtacct aaatacgcat ataaagcggg gtaatttgct gccaaacatt caatcaatct
     8881 caatcgataa ccgtctttat aaatattcag acgcgtttct gttgaaacta acttggttgg
     8941 cactatggac tctttaattg atccctgccc ggaataaatg aattcttgaa attgggtttg
     9001 taaagtcaat agcgatttca tacggctacc ttttcttttt cagcttgtat ggcaaggcgt
     9061 tttgcatgat taatttcact taaaagctcg ctaaaatcag gcatgttatc atcacgttca
     9121 atcatagtgg atacaggccc aagtctctgt atggttgcct cataaagatc ccaaacttgc
     9181 tcaatcactg gagcattgtg agtgtctata atgtaatctc cgtgattagt gtgacccgcc
     9241 aaatggattt gagctactct tccaggaggc atggaattga tataagccat tggatcgaaa
     9301 ttatgattga atgaactgac ataaacattg ttgacatcca acaataaata acaaccggat
     9361 tgttttacta tttccagaat aaaatcccat tctgacattt ctgactgttt gtatgtaagg
     9421 tagcttgata cattttcaat taaaaaagga cgttttaaga aatcttgtat ttcttgaata
     9481 cgggatacga tgtgttgaat agcttcagtt gtatagggaa ccggtaataa atcatgcgta
     9541 tttaatccat gaatgccagt ccagcataaa tgatctgaaa tccagacagg ctcaatacga
     9601 gaagctaatt cttgtacctg cttcaaataa tcccaattca aaggatcagt gcttcccaga
     9661 gaaagagaga cgccgtgcat gacaatagaa tagtattcgc gaattttgtc cagataatag
     9721 agaggcttac ctccggggat taaataattt tcagttaata tttcaaacca atctagatca
     9781 ggtttgctcg tcaggatttc ttcataatag tttggtcgta atcctaaacc aaaacctaaa
     9841 tacggtaatt tttgatctga cttatttggg gatacttcca tattaaacct aaaaaagaat
     9901 ggggaccagg catgtttttc tacatgcctg gcaagatgcg actgaactaa ttgctattat
     9961 tcagcaacag taccaccttg tttttcgcaa tcttctttag acatctcaag aatacctttg
    10021 cctttgcaag aattttgacc tttgcaagca ttatcagcag ttttacagct gccttgacct
    10081 ttacaagcat taacaccttt acagtgaatt gttgtggttg tagcatcaac agcaaatgca
    10141 ggagccaaag caaacataga agccgcgcca acagctaaag cagcgcctgt taattgtaat
    10201 ttattcatag caattccctt ttgattaaat gaaaatgatt gtaaacccgg caaatctgaa
    10261 ttgttttgcc aatagcattt taagaatcaa taatcaaaaa agtcaagatt atccaaactg
    10321 ataaataatt attttttcaa atagttaagt aaattattta attacactac aaattttgaa
    10381 agtactttat atcaccaact cccgccgttt aatcgaacag attcgggaga caattgaatc
    10441 aatttgcgat tttctaactt ccggatcatt aagcggaatt gacctctaat cctttcaata
    10501 aaagccatta tttctgcacc ggaaaaaatc acacgggaat actgcgaaaa aactggaata
    10561 tgaatgattg ttcctgtttt ccgagtctct gtagctcttg ttcagaatat taaatcgaat
    10621 gttattttgg ggattcgtta atccaaattt actttttcaa ggtagttagg tatcttgcaa
    10681 ttccatccta gctccgcttc aatatgtccc tttaacccta atgccgcttg aggctcgcca
    10741 tgggtaataa aaacttcctt tggggctttc tcaaaatgct ttagccaaac tagtatttcc
    10801 tcataatctg cgtgagcaga ggcattggtt aacatagcta cctgagccct gactggataa
    10861 tcatcgccat ggattttaac ggacggctca tgatttaaca ttttttcacc cctcgttcca
    10921 gcagcctgga atccggtcaa aaggatagta cttgcaggat caggagcata taccttcaaa
    10981 tgatgcaaaa ccctgccccc tgtcatcatg ccacttgcag aaataataat tttagattcg
    11041 cgcccccaat ccactgccat ggattcattt ggggtatgga cataagtcgc tatttggcag
    11101 acggctcgac actcctctgc acttaagcga tgttcgttat gatgttttaa taataattca
    11161 gtggtatcaa tagccatagg gctatccaga tagacaggaa catcaggaat taattttttt
    11221 tgttttaaaa tatacaaata atacaacata gtttgtgccc taccgactgc aaaagcagga
    11281 atgagcactg taccacctct tttacaagtc gtattgacta tgttggctat aaacgctaaa
    11341 ggatcggttt tatcatgcaa gcgattccca taagttgatt caatcaccaa ataatcggta
    11401 gatttcatta actcggaagg cttcataaca ggatcattga gtcttccaat atcaccacta
    11461 aataataact tgatttgctt tgtttgaaat tgaataaatg cagaccctaa tatatgccct
    11521 gccctatgcc aactgatttc gcaatcctcc attaaaggat aaggggtgtc atattctact
    11581 gattcaaaat actttaaagt attatttgca tcttcctggg tatataaagg taaagccgga
    11641 tggtgtttac tatatccgta tttatttgct cttctggcat cttcctcatg caaaaagccg
    11701 ctatcgggca gtaagataga gcataaatcg gttgttgcac ctgttgcata gatgcgcccc
    11761 ttaaatccct gtttcaccaa taatggcaaa taaccactat gatcaatatg ggcatgggtc
    11821 aaaacaacgg catctatcga ttttggagac acaggcaggg gtgaccagtt tcttagtctc
    11881 aattctttta acccttggaa aagtccgcaa tctaccaaaa tctttttatt atttgattct
    11941 accaggtact tagaaccggt aaccgtaccg gtagcaccta aaaaagacaa tttcataata
    12001 ataccttgtt gtatgtcatt ctcagtaatg taatgaataa cacgacctat tgccaattta
    12061 ttttaaagcc atttgaagat tcaaattgta aatctttatc ccacctgttc acttgcactt
    12121 acggtgtgga ggcttttata aaactgcttt ttaagttttt tacccacttg tctctattct
    12181 ctttagtcat atccgatttt ttaattgcat aatgaagcac ataaattcca tccttgccct
    12241 tggtaatttt cagaatttcg tcttgttgca gattggcagg ctcttgaatt ttaaactcaa
    12301 aaatgagtag attgggttgc tcctcaatga catggacaat atattttacc cctgatttat
    12361 tcaggttgtg aaaaaactga ttaccaaact gagctggagt catgttgatt aaacccggca
    12421 taaattgtgt agtaatcaac tctttccagt tattaatgtc atctccaaca ggaatatact
    12481 caatggtaac caccttatct gttttctttc cccaagcgat cttccatggt ctgtcatcaa
    12541 aacgtactat ttgattctca taatggataa cttgtgagtc acctgaggaa tcagccaaag
    12601 gaacaacact gcaataaagt tctcctttat aggttacaaa ctccacacat ttaggattat
    12661 tttctttatt gactttgatt aattcttttg gaagagaagt gccattaggc tctgcgtagc
    12721 aaataacggc attaagtaat ccaaaacata acccaatgaa ataattcatg ctcttaatcc
    12781 aaaaagtgga tactatttaa ttgtagacgt tgaaacccat aacccacaat tttcattagg
    12841 caaactggta tataattata ttagagagct aataaggaga ttgccatgaa tggttgcaaa
    12901 atcagtcctg ttggactggg tctttcttta ggaattctat ggggattatc attgttagtc
    12961 atgggattaa tcgcctattt ttatgcctat ggcaggcctt ttgttgaagc agttgccacc
    13021 ttataccttg gctacgaacc ttctatcctg ggaagcttca ttggtggcgt cattggcttt
    13081 attgatgggt tcgtcacggg cttccttatc gcttggcttt acaattgttt tagttgttgc
    13141 tcttgttgcc gaggcaaaga aaataaaaaa gaataagctt gctaataatg agaaatcatg
    13201 acgtcaatga cgttaaaatc aacgtcattg acaagagcaa ttaatttatt cgagagttaa
    13261 aatcatttat gggcgaacaa taatacggtt ctctactgac tgaacccctt gaacttgcct
    13321 ggcaatttgt tctgcgatat cgctctggcg aatttttttc acataaccac ttaatatcac
    13381 cacattttga ttggattcca catgaatttg attgatcaca ggatcattac tttccattag
    13441 tgaatccttt accgactgag ccaaagtcat gcctgatgga ttagatggaa taaacatgtt
    13501 actcacagta ttggtctgac aacctgctgt aaacaaaatc aaaaacacaa tgattacact
    13561 ggataaacat tttagcatca caacctccaa gatagagtgg atttatttac accgcattaa
    13621 aagaaccaaa taatagcaca actccccgta tttctatagt caagccactg cctataaatc
    13681 atcgtttgcc aagagaaact taagtctttt caaacgataa ctcagattaa attgtcattt
    13741 atggaatgct gctatactat tttttataaa tcaaacacct acaggacgca aggtatgaaa
    13801 aaaacaaccg aaaaacctat caataaaaca aaagaaatgc ctgcatcctc aaaaaaagaa
    13861 atcctaaatc cactggagca aacaccttct cttgaacaat ccgatcccat tttcagattc
    13921 attgataagt tatatcaagc taacctggga aagttaactg ctggtataag tcctgctgcg
    13981 ctaggtacag cctattactc ctggtttgct caattgctac agtcacctgg ttcaatgcta
    14041 cgactggctt attatccgct gttgcatgcc aacgattatc tttccaacct ttttaagtat
    14101 gacaagccca gagatggcaa ggacgtacgt tttcatacag aaaactggag ttattatccc
    14161 tggcggcttt gggctgagca atttctccaa tttgaagatt ggtgtttaca agcctcaagc
    14221 aaagttccag gtataccact tcatgtcaaa agaacagtaa ccttttccac cagacagatt
    14281 cttgacgcct tgtcaccttc caattttgtt ttgactaatc ctgatttgct tcaggaaaca
    14341 attcgctcga atgggcaaaa tttaattcgc ggaacagagc ttgcctttca ggattttgta
    14401 gaaaagataa caggctcacc tcctgcggga gtcgagaatt ttatcccggg gaaacaagtt
    14461 gctgtaacaa aggggaaagt tgtttatagc aatcatttaa ttgaattaat ccagtacaca
    14521 ccccaaacag aaaaggtata taaagaaccg atcttgattt tacctgcctg gatcatgaaa
    14581 tattatatcc tggatttgct gccggaaaac tctttagtaa actggctggt tagacaggga
    14641 catactgtat ttattgtatc ttggcgcaat ccaacgaaag aagatagaaa tcttggactg
    14701 gatgattatt ataagttggg cgctatggat gccataaatg cagtgagcaa tgccattcca
    14761 cataccaaaa tccatttaat gggttattgt cttgggggca ccctggctct gttaactgca
    14821 gcagcgatgg cccatgatca cgataaccgt cttaaaacat tatccttgct cgctgctcaa
    14881 ggcgatttta ttgatgccgg agagctactg ctctttataa ccaaaagtga agtatcgttt
    14941 ttaaaaagta tgatgtggga gcaaggatat ctggacacaa aacaaatgtc tggaactttt
    15001 caaatgctcc gttcgtacga tttgatttgg tctaaaatgg ttcaggatta catgcacggg
    15061 actcaaagag ggatgatccc tttactggca tggaacgccg atgcaactcg catgccatac
    15121 aaaatgcata gtgaatacct ggaaaaactg ttccttaata atgattttgc cgaaggccga
    15181 tttattctgg acgggaaacc tgtcgtaggc gaaaatatta ggatacctgc ttttgttgta
    15241 agtacagaaa aagatcacgt agccccctgg aaatcagtgt ataaaaccca tttgttaatc
    15301 aattccgata ttacctttgt cttgactaat ggggggcata acgctggaat tgtcagcgaa
    15361 ccagggcatg agggacgtta ttatcgtatt cgagaaagaa aaatggattc aacctatctt
    15421 gacccaacaa actggctaaa aaaggctgaa ttaagagaag gatcctggtg gattgcctgg
    15481 cacgattggt tagttaatca ctcatcccaa aaacaggtgt ctgcgcccaa actggacaaa
    15541 aacttgccca atgctcctgg aaaatatgta ttacaaaaat aaaggaatta gtaatgaata
    15601 atgaatttct tgaaaatgtc acctatgaag aactgactat agggcgcaag gccagcttaa
    15661 accgtacatt aactcaggat gatattaatt tatttgctgc aatgtctgga gacatcaatc
    15721 catctcatgt cgatcctcaa tttgctaaaa gcgatgtatt ccatggcgta gtcggacatg
    15781 gcatgtggtc tggagctcta atttctacct tattgggcac tgcccttccc gggcccggaa
    15841 ctatctatct tgaacaagat attcaattta taactcctgt tcgcattgga gataatctta
    15901 caattaccat cgttgttcgt gacaaacatc caggcaaatc gattgttcat ttcgactgca
    15961 tgggcgtgaa tcaaaaagga gaaacagtaa ttaaaggcct tgccaaagtc attgccccga
    16021 ctaaaaaaat gaaggtaccc cgaactgttt tgcctctggt gcaaatccat cacaatgatc
    16081 actttaataa aatcattgaa gcctgcagta acttgccctc aatcaaaaca gcagttgttc
    16141 atcccgtcac tgccaatgtc ctggaagcag tttatgattc agtcaaggct ggtttaatta
    16201 ctcctgtact aataggtcct atggcaaaaa tcaaatgtgc cgcaagtgag gctaaaatcg
    16261 atttatccaa ttgggaaatg attgatacag aacacagtga tgctgctgct tttaaagcag
    16321 ttgaattagc ggcatccggg aaagtagatt ctatcatgaa aggcgccctg catactgatg
    16381 agttaatgtc tgcagtggta cctgccactt ctggactaag aaccaaatac cgcatcagtc
    16441 acgcctatgt tatggatgtt ccaacttacc ataaaccttt aatcattact gacgcagcca
    16501 ttaatatagc cccaaacgca gcagataagg cagatatatg tcaaaatgca atcaatcttt
    16561 ggcgtatttt atatggagaa gaaataaaac ccaaggtagc tatacttgca gccgttgaga
    16621 tggtaaatcc aaagatgcag gcaacagttg atgccgctgt cctgtgtaaa atggctgacc
    16681 gagctcagat agtcgatggc atactggatg gccccctagc ctttgataat gccatcaata
    16741 aacaagccag taaggaaaaa aacattatat ctcctgtagc cggtgatgcc gatattttgc
    16801 tggtaccgga cattgaatca ggaaatatgc tagccaaaca actcactttt ctcggacatg
    16861 ctgacgcagc aggaatagtt ctcggagcaa gagtccctat cattctggcc agcagagctg
    16921 attcgttaag aacacgtttg ttatcgtgtg cagtagcaac caagattgca gcggcccgta
    16981 aagcaggcaa aataaaatga ggcattctgc actgttaatt atcaattcag gctcctcaag
    17041 tgttaaattt caagtattct ccaatacaga gaatcttgat cttctcgcca aaggcaagat
    17101 tattaatatt ggaggagaac catcttttac cgcagttaac gaaacatcga agcaccagag
    17161 aactctaatc actttggaaa agggcatgac gtatgagcaa gccattcatc ttatactgga
    17221 ctggattagc caacagcagc aaaactggga aattcatgct gtagcccatc gggtcgttca
    17281 tggcggagaa tacttcactc agagtgtcct ggttactcca caaatcatca gccaattgga
    17341 aacactgaat tctctcgcgc cactgcatca gcctcataac ttggccgcca taaaaatcat
    17401 taagaagatt aagccggaat tacctcagat tgcatgcttt gatactgctt ttcatgctca
    17461 acatactcca ttatttacta cctgtgcttt gcccgaatat ttaagacaac aagggattag
    17521 gcgttatgga tttcatggtc tttcctatga atggatttct cttgttctca aaaaagacta
    17581 ccatgatttg gctaacaatc gaattattat tgcccattta ggcaatggcg ccagcttatg
    17641 cgctatggat aagggagtca gtatagacac caccatgagt ttgacagcac tggatggttt
    17701 accgatggga actcgttgtg gcagtttgga tcctggggct gttatttaca tgatgagaga
    17761 attgaatctt ccaccagata agttagagga aatactctat aacgattccg gcttgcttgg
    17821 tttatcagga aaaaccaata atgtgctgga attacaatcc agccctgata aaaacgctca
    17881 atttgctttg gagtattttt gcctgaaaac agctcaattt attggaatga tgaccatttc
    17941 gctaggtgga gtagatggta ttgtattcac aggaggaata ggggaaaatt cagaatcgat
    18001 tagagcaaat atccttaaga ggatagagtg cttacaccct ttttctgtta tcgttattcc
    18061 tgcgaatgaa gaaaaaatga tggctatgca taccttgaca gccctcagga cgtaaaggcg
    18121 tatttttact ggcataggta atgagtataa aaaatggata aagactacta taaaatcatg
    18181 ggagtcagcc aggatgcttc tgataaagac atcaaaatgg cttaccgaaa attagcccgc
    18241 aaatatcacc ctgatattag taaagaacca gatgctgaag aacgatttaa agaaatggca
    18301 gaagcttatg aagtactgaa agaccctaaa aaaagatccg aatatgatca atatctgaaa
    18361 tacaaggaat tcaatcccca aagcgatgga tttaccgggc gaagaactca agaacaaccc
    18421 tttcaggaat ttcattttga cagcgatttt tttgaaacct tgtttgggca ttcgcgctat
    18481 caacagcaac cgatgactgg tcaaaactat catggcaaaa tcactatcag tctggaggaa
    18541 gcttaccatg gagctgttaa aaacttacaa gttcctgttg aacaaggcac agagactaaa
    18601 atacagaccc tgaaagttaa aatccctcca ggggttaaat caggacaaca aattcgcttg
    18661 gcaggacaag gaggcagtgg aagcggaggg ggggctcgcg gcgaccttta tctaacagtc
    18721 gaggtgatga aacaccccac atttgatgtc atggagaatg atatttatct cacattacct
    18781 atcacaccgt gggaagctgc tttaggagct acggttgtca taccaaccct ggcaggaaaa
    18841 atcgatttaa aaatccctcc aggctcacaa ggcgggcaaa aactcagaat taaaaatcgc
    18901 ggattaccag gttcaccccc tggtaatcaa tatgttctat taaaaattat taccccccct
    18961 gctcaaactg aagaagcaaa agctttatat aaaaaaatgg cggaggtaat gccttacaac
    19021 cctcgtaaga ctatgggagt ataagcatga gcaaagacaa tcttttaata ggcatgctta
    19081 tcgaagaaac cacaacgatt tcttttattg aagtatgcca aaagtatcat attcctgaag
    19141 aattattcat tgaaatgatc gaacaaggtt tgtttccaga tcaaccatct gatcccgaaa
    19201 aaattgcatt aggccaaaaa gaattgcgtc gccttgaatc cgcctttcga ttacatagag
    19261 atttaggcat taacctgcct ggagtcgctt tagctcttga tttactggaa aaaattgatc
    19321 aaatgcatca tgagcttgaa attttacgca agcattttta ggcatcagaa tctttaggat
    19381 tataatcagg tatctcttta gggcgataac ctttaatcgt tctaaaaaac tcgataatgt
    19441 actgaaaatc ctttttaata tctcctgtgg tccaaaaagg ttcatggata cggatttctt
    19501 tagtgaaata atcaggcccc accatcacta taggaatacc tgcttgccga gcaatatgat
    19561 aaaacccctc tttccatctg gtcaccggac ttcgggttcc ttcgggagcc aggcccagct
    19621 ttaattcatc gcgttggcga aacaactcta caactttctc aaccctgttc ccctttttag
    19681 aacgatcaac cggggtgcct ccaactgcac gcaaaaaata gctcaaaggg aaaaaaaata
    19741 actgatgttt cgctaaaaaa ttgattttaa ccccgacaac aaaacgggca cacaagcaaa
    19801 tgacaaaatc ccaattgctg gtatgcggag caacaatcac aatatatttt ttttcattcg
    19861 gtaattcacc aactatcttc caaccgatta atttaaaaat aaaacgacat attttttgca
    19921 tgacaaaaat ccatttttgt tttaaccaaa gtatacttga ttttgatatt tatttagaag
    19981 aaattgttat ttcacatgat caaggtacaa tatccctttt ttcgaaacca cttaccctat
    20041 gctctatcat gatccattga tttcagaaca caccatgaaa tgggttcgga cattcattat
    20101 taattacacg atttgccctt ttgcaaaagg ccccgtaaac aaaggcactt taagaataat
    20161 tacttcagat accctcaaaa aacaccaggc acttaaagat ttaatcactg aaattcaatt
    20221 tctggaagca aaccctgctg ttgaaacgac tctgcttatt tttgctcgag catttaaaga
    20281 ctttttttct tatctggatt tcataagctt tgctgaacaa ttaattcaaa acttaaatta
    20341 tgaagggata taccaactcg ctactttcca cccggattat tattttgctg atacttctcc
    20401 agatgatgtt gccaactaca ctaatcgctc cccttatccc atgatccatt tattaaggga
    20461 agacagcctg gacaaagcaa tcgctgccta tggagatacc cacattatac cagaaaaaaa
    20521 tatagcgact atgaatgagc ttggtttaga aaaaatgaaa cagttattgg cttccatttc
    20581 tataccctag tatgcaacag gacttccgaa aaagaccatg ctttataatt cattcttata
    20641 aatgacccca tctttcataa tcaatttaag attttcatcc gggcggagca gaacatttaa
    20701 attgactaaa ggattgccat ctaccactaa cagatcagca taagctcctt cgcttatgac
    20761 accaagtttt cccttttgca ttaataattc cgcattgact acggtagctg aacgaatgat
    20821 attggctgct gaatcaagct ctttgcgcat ttcaaactct cttaactgat ctctctgtgc
    20881 ctcaggaccc cacaaatccg taccaaaagc aataggaatc ttatatttta aagcatattc
    20941 ataaaccttt ttgccttttt caatagtatt tttcaaattg gctacaatag ccttagacaa
    21001 ccgattttgt tcagcacttt caattctttg cgccaatgaa ataaatgtcg gatcgtaaac
    21061 cacaccactt tccgacatta attctacagt tggttcgctt acaaaattag cgtgttcaat
    21121 ggaacgcaca cccgatttaa tgcactttct tactgattca tcgctataag catgagccat
    21181 aacataagta tttcttgcat gtgcctcttc aacaatagtc gataactcct cctcggaata
    21241 ttcatacagt gttggattgc taagggaagg gaaaacaact ccgccagaag caaatacttt
    21301 taattgtgtt gctccttttc ttaattcttc ccgagcagcc tttctcactt catcaacccc
    21361 atctgcaata accgacatcg tcgagaaaga actaacatgc ccgcaaggat caatctgttc
    21421 attcggtttt ctaaagtcag ctcctccacc cgtttgagtt aatgccttcc cggaataaaa
    21481 taatcgaggc ccaggaatac tcttattatc cagtaattgc gcaattctaa aatcagcgcc
    21541 tccagcttct cgtaaagtcg taaaaccata aaacaagcta tttttgagat aattagcggc
    21601 agccaaaaat atctctgctt cggaatagaa gatatttttt gggcttagag tcaatccggt
    21661 aacatgagcg tgagcatcga ttaaaccagg cattaatgtc ttccctttga catcaataac
    21721 tcgcattcct tcgagaggtt gaagcggtgt ttgcgacacc tgggcaatca aatcattttg
    21781 taccataaca tcaaaatttt tttgaatctc tgtagattct cccaaaataa cgttggcatt
    21841 tttaaaaaga attgagctca ttttaatctc gaagtcatta atctaactta ctctatttat
    21901 agtctatttt ggcaactctg actcgctctc atttttcttt accctgcttt ttcaaaagta
    21961 tttaatttaa cagtgcccca tagaaaataa tgaaacccga gtccacatta ttgaaataat
    22021 gtactaatat ttatcatata gaacttatta tcaggtgcat catgaacaca caagatccag
    22081 cgctttcgat ctcaaatcca cggttattat cagcaatcta ttttggcttg ttatcggtcg
    22141 ttggcacgat actgatcaat gcattcctca cttcgattgg aattgaagaa attatcccgg
    22201 tctatcaatc ggtcattctt ggaatggttg tagcttcctg caccggggct atttttgggg
    22261 aagccatcgt tcactgtaaa aaaccttata aaagaaaaac cttttggcta ggattcagta
    22321 tggtcatagc ctcactgcct gtctttgatc tcggccttgt cttcttgatg tcagaagcaa
    22381 atgccaagct ttttgaagta accgatatag gcagcatggt tttgttctat ctagttgttt
    22441 tggcctatag ttatgtgctt tttggtattt tgctggctgt tgcatccgga ctagctgcta
    22501 tgtatttaag agggcaatta gtatacgata tcctgcatac ccaaacccga cgtcaacaaa
    22561 aacagaagca tgcattggca aaaaataaat cagaaggaca tgcccataaa gttcaccgat
    22621 gaacttaagt cagatgagct ttataatcta ttgtctcaag gagcagtcgt tattactcct
    22681 aacaatagat taagcggaac catccttcag aattacttca cttattgttc cagaaaaaca
    22741 attaccaagc caatatgctt gccttttaat acatttctag ttaactcttt tgaacaactc
    22801 aaattccaaa aaccagacct ggaacatccc atacttttaa actctgcaca atgccaacac
    22861 ttatggcgaa aactaattaa atctcatcct tctatcattt acacggaagg cttgcttaaa
    22921 acaataatgc aagcatggga atactgtgag caatggcaac ttacacctga agcgcctgct
    22981 tttcactaca cgccgcaaac acaacaattt caacaatggt ggcaatcttt taataaaata
    23041 cttaaagaaa tttctgccat ccatcagcat caattaattc gctacttgct tgattccaat
    23101 taccaagggc tcgtaaaaac cgttgtctgg atgtgctttg acgactttaa tccacagcag
    23161 ttatccctgc aaaattattt gaacgcgcag ggggtaattc aatatggtta tgatttaaaa
    23221 gaaaaccagg agactccgga ggttttctca gctaaagaca aaaaaacaga ataccaggaa
    23281 ttgatgacct ggttggagaa gaaaattcaa caaggaaaaa agcaaatcgg ggttgttgtg
    23341 cctaatcttg aacaagaatc atcggttttg caacgagttt tacgcgactg ctttgaaccc
    23401 aatttattta atatttcttt aggcaaacca ttaagcgcat ttcacttgat ctctcatgcc
    23461 ctcatttggc taagtcttga tcacaatctc ttaaataatc atcaagcagc tttgctgctc
    23521 caatcaccct acattggcaa ggcaaaagat gagtttcttc aaagatcaga ttacttacag
    23581 gaaagcaagt tgttgcaaga ccaatctttc cccacaaaat tatttattga ggacgttgcc
    23641 taccacacgc ctaaactggc tgaattgtta aaaaacatga atttttatcc ggaaaaaact
    23701 accctggatg agtggattga gttatttcag gagaggttaa atggcatcgg gtttcctggt
    23761 gattatgttt tgaactcaga ggattatcaa tgctttaatc gctttacaat gatttttgac
    23821 gaattcagac aattaagtct gataagcagc catttgacta aaaaagaggc atttgaaacg
    23881 ttaaaacaac tcatagacaa cactattttt caaccacaaa aaagcaattc accaattcaa
    23941 attttgggat tgttagaggc atcaggttgc gaatttgaca gcctttgggt aatgggattg
    24001 actgatcgat gtttacctca acaaacacgt ttatcgccct ttatcccatt tgaattacag
    24061 cgtgaactgg gcatgcctca cagtatgccg gcaagagagc tccaatatgc caagcaaatt
    24121 ttgcaaagac tgcaacgagg cagcatggaa actgttttta gttatccaca atttgacgat
    24181 gacacgccta atttaccctg ctcattgatt actcattatc ctgcctacct cagccggtct
    24241 gctgacaata aaacaaatgt tcaaagttgt ttggttcctc ttgaagaaaa ctatgaagta
    24301 cctgtaaaat ctgatgaatc catttctgga ggaaccagct tactaagcaa tcaggccaaa
    24361 tgccctttta aagcattagc acaacaccga cttaaggcaa accctgttct aaaaactaca
    24421 gatggaatgg atgacaagga aaaaggtcaa ttaatccata aaatcctgga gttgctctgg
    24481 caaagattgg aaaatcagga acacttaatt aaattaaaac ccgctgaact tgaacgattc
    24541 attgatgaag caattcaagg cgctttaaat ccgattaggc aacgacactc ccaagctttt
    24601 cctgacttga ttgccaatgt tgaatacatg agattaaaac gaattatctt gtcttatctg
    24661 gaatgggaaa agcaacgccc tccttttaaa atcacaggcc tggaagaaaa tcattcgatc
    24721 aatttggctg ggctcgattt taaagtaaga gtggacagac tggatgaagt aggtaacaaa
    24781 caatgggtca ttgattacaa aagcaccctc cctggcggca aaccatggaa tgaagaaaga
    24841 ccaatggagc ctcaattgct actttatgcg ttgcttaacg aacaaattaa tactttgtta
    24901 ttgattcaat taaaaaccgg aaaaatcaag ctcagcggtt ttagcgaaga aaaatgggaa
    24961 accaaagaga ttaatacttt gaaagaggat gaaaattggg aagactatcg acaacgctgg
    25021 ctagaacaat taactcattt agctgaagaa attaagtctg gctattgtcc gccacgccct
    25081 caaaatctga ccatttgcca acgctgtgat tttcagaact tatgtcgctt ccaggctaaa
    25141 caataaacca gttttaattt atcgggacta cttatgcatc atttaattct tggttatggc
    25201 tattgcggtt attaccttgc ccaagagctt cttaggcatc atcaacaggt aaccgtagta
    25261 tcacgacaat taaaaaagga attggaactt tcaaaactac agcatataag ccatgatctc
    25321 gaatccccat ttcattggag cgagcccgac tccattatct actatctaat ccccccgcct
    25381 tctcaaggca cctgcgatac cttcttgcaa cagtttttaa atcaaagttc attaaatgca
    25441 aagaaaatta tttactttgg ttcaagtgga gtctatggaa accatcaagg agcctgggtt
    25501 gacgaattat cagcttgtat tattgactcc ccacgtcaac aaagacgatt ggatgcagaa
    25561 caacaatggc tggcctattg tcagcaacac aataaagagc tttccttact gcgtattgca
    25621 ggcatctacg ggccgaacag aatcccagcc gatgcagcca aatcgaaaat cccaataata
    25681 aaaccgactg aagccccttt taccaatcat atttttgtca aggatttgtc tttaatagct
    25741 tatttgcttg gccaaaagca aacaacatcc gctgtctata atgttgccga tggcaaccca
    25801 acccatatgg gcgaactaca acaaacggta gcaaacgcat taggaattga atcagcatcc
    25861 tatgagtcct ttgagcaagc ttgggaaaaa gcaagcccta tgaaaagaga gttcatgcga
    25921 gcgtccaaac gattgaagat tgatctgtta aaaactgcat tgggtgaaga tattcagctt
    25981 acctgtttgt atgatgcagt aaaacaaagc ttatgatttt ctttttttgt aaaaaactat
    26041 tcttgctaag ttaaacttta attttcttta aaatatggta ttatgctgcc acattggtta
    26101 tatataattc aataattcgt tataaatcaa taggatttta aatttataat tacaattgag
    26161 gtaaactatg ccttcttaca acaatgcccg gctcttggaa aaacgcttgc aaaattgtga
    26221 tacacaatta aatcaatttt ttaatggtca ggaattatca ataaagtttc tccaacaatt
    26281 aaatcaaatc aagtattttt ataattcagc tttcagcaag accgaaaatg aagacgatgg
    26341 cctagaagtc gttgaggagt acgaaaattt catttcgttg gtctcacagg taaaatctgg
    26401 acaaattaat gctgacaaag cgtttgaaac aataaaagat acgacagaat ccaggcaggc
    26461 agacgttatt attgctaatt tctttaaagt ttgtgaactc atgttctggg ctactgcagc
    26521 attttttagt tatctagcct gtgtatcagt aggtatcccc ttaacttttt gtgatcctct
    26581 cctgggtatc gcagtctcct tatctacctt tttgatgaca attagcacag caagcaaatt
    26641 tttagactgt tttgatgagt tccaatcgtt agataagatt aacgagcatg ataaaaatca
    26701 aaaggatacc gtacggtttt tcagtaaaac gtcaaactcc acttcatcgg tacgcaataa
    26761 agaagctgaa gtagaagaaa gctctcagag attatatccg aatttacaaa ccgtctaaat
    26821 taataagcaa taccacatca gatcaaagat tattttggtc tgatgttttg gtttgcttta
    26881 aagagcataa tcaaaattaa agcaatactg agtcaactca tatttaaatg ttctctatac
    26941 agccgttccg ataggaggct accctgttac aaattaatct ctgaaatctc tctattcatc
    27001 cttaatcacc aaataaatca taacggctaa aatagtaatg aagatatgaa aagctgtggt
    27061 aagagctgag ttgagcatgg gagacatcca cataccaaac cactctcctc ctatggacat
    27121 aaaaatcact tgccaggtta caaaacccaa cgtaagacct ccaatagccc agttcttgga
    27181 atgattaaaa acagaagcac tttcatttct tgccttaaac aatttccacg caccaactcc
    27241 gcaaaatatg gcggttaata attccaggat aacaatgata ataaatgcaa cataatgcag
    27301 cgctggacta gtaattgctc gataggtgat ggtggaatta ggaaatatat cgctcatggt
    27361 taatactttt accacagcag gaaaattggc agaataatca gtgatgtttc caaatgcagt
    27421 aagtaaacaa aaaaaagaaa cagcaacaac aagcaacact tttgagaatc tgataatcat
    27481 catttcatcc tttgaaacaa gttaaaaaat atgtatcacc taattgataa gagcgttcgt
    27541 ggagaggatg cttatgctcg tttctcacca agccaggttc gctttcttca agcttcgcta
    27601 accaaaccga tgaatcaaaa aatcttagcc caaacctttg cccactttat catcaactac
    27661 cctttctctt tgagtatttc tggcaaaacg taacaataaa tgattcgctc tgatcgtcgc
    27721 ctcgttcaga gcttcctccg gactcatcaa atctgcaaac atcgcctcta aaacttcatc
    27781 attaattcca cgaatttgat tttgtgggcc catgtgttta taaggtttct cagataaatt
    27841 gtcctcgaga tctgttctgg ctaataataa agcgggatgt tgacttgatt gcacaatgtt
    27901 agcgtaaatt ccttttaacc ccaacggcaa atagcccgta tgctcatgcc agcgcttttg
    27961 cacttccggt tttgcaataa atgcaaaaaa tctggcaatt cctcgatatt gctcttctgt
    28021 ttgtcctcct acagcccata gagcagcacc ccctgccaca ttcgcatgtc ttatcggact
    28081 ggcccctgta tccaaaggca ttgttgcaac acccaaatga aaaggaacaa gggcagacaa
    28141 actattataa gcacccgatg attgactaaa taaaggacaa acactactgg taaataaaat
    28201 ggtagcatca tcaacacgac caccataacg aaaataatgc aaatcatgcc agcgctttag
    28261 tcgttgaaaa tgagccatta attggggagt atggaaagca gcacgagcag gattgccttg
    28321 agttatgggc aagccatgga tagcaagaaa agattcaaat aacacccatc caggataagc
    28381 cgtggtataa gtacagtcat aacccgcttt tttaattttt tctgccatta tttccattgc
    28441 tgaccaggtt tgagggaagt tatacttgcc atatcccacc ttggctaaaa tgtcagcatt
    28501 atagtacaga actggtgccg agagattgaa aggcatcgcc attaattgcc catccctgct
    28561 ataaaactca cgcactgatt gaataaaatc atccttaggc aaactgatcc cctgctcact
    28621 catcaacaaa tcaacgggtt tgatcacacc agggggagct agcataattg aagtgcctac
    28681 ttcaaaaatt tgaactattg atggtgcttg acgagctctg aatgcagcag caaaattggt
    28741 aagagtttcc gtataatttc ctttgtaaat cggtttgact cgatattgat tctgactttt
    28801 attaaaatca tcggcaagca aacggacttc atcacccaaa tgccctgcca tcgcatgcca
    28861 aaaaaccagc tcaactggtt tggcttgagc aggaatgaca ctcagaaaaa ttaacaaaag
    28921 caaaaagagt attttcattt taacaagtct ggataatcac taaagagagc atcaacaccc
    28981 caatcaaata actttttggc caaacgttta cggtttacag tataggcaca tactacatag
    29041 ccttgatcct taactgcctt cactcgatca gcggttaata tctttctatt gaaatgtatt
    29101 gaataacatt ccaattgttt cgccttctgt aaccaatctt tatcccactc atgcagcaag
    29161 agccctaaag gcatttctgg agcaatactg cgacataaca ccaaagcatc ccattcaaaa
    29221 caggaaacga gaggtaattc tttattttgg ggccaatacc gatggatata acttagcact
    29281 gatacagtag tttgctcaac tgttccagga taaggtttta tttctatgtt cgcctggaca
    29341 tcggaaaaag acaaccactt taatacgtca gtaaagtgtg gaactttttc tcccttatac
    29401 tgcttcgaat accaagagcc agcatcaaga ctacggagat aatctgcctc caccagtccc
    29461 acctctcctt ttccattcgt ggttcgtttc aaattgtcat cgtgaatgat aaaaggttca
    29521 ccatcaagac tgcacatcac atcaaactca atgaaacggc aacctaaaga caatgcttta
    29581 ttgaaagacg ccatcgtatt ttctggagca taacccgatg ctccccggtg gccgataatt
    29641 ttttcaagga tcaactaatt aatctccctg tttgcgattg gatgctgctt ctaccatagc
    29701 ggtcaatatt aattcattac tcactgtcaa tggtgccgga gcagcagcca tcactaaagc
    29761 attcatttct ttcgcttgat aggctcttgg ttgctgggga atgttgtcgc cttcgaaaaa
    29821 aaccaggtta ctcacagagt aattttgctt gggataggct ttattcatac gatcaatttc
    29881 atcattcact tgctgataaa gctgttcacg aatcttggca cgtacgactt gagtctcttc
    29941 caaactgggt ttgaactcca caccactgat ctcgtacttg gctccaggtt tactgaccga
    30001 tttggcattc tgataaatat tagtcagagc agattgatcg actcttgcct gagcttgtac
    30061 ataaagtttc tcaaggccag aactatcctg agaacggtca aactgcacca aatgccactc
    30121 ccctttagca attttgttta agctatccat tatgtcagca cgtgctttca ccaaatcagc
    30181 attacttaga gtggcattga tactgacatt gagtaacgct gtttgggtag taacccattt
    30241 tttagcagaa acttgaaaaa aaatcttatc aagcaccaaa tctggtgtct cattatcagc
    30301 ccaaacaaaa ggagttaaga ccattaaacc cagtatccag gcaatttttc tcataatatg
    30361 ctccattcat aatgtttcta ttggaattag tgtataccag caagctagaa gacaccacag
    30421 cagtttgaac ataatcactc taaaacaaat cctatacggt attgcccgca aacggtcgga
    30481 tttgagcaca ggttgagaaa ataagcagag taaaaaagcg cggtgtacat tgaagtacat
    30541 gagcattttt tagaata
//
LOCUS       DAXLDT010000022        29517 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXLDT010000022 DAXLDT010000000
VERSION     DAXLDT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29517
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.022"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..282)
                     /locus_tag="ACF2SP_000790"
     CDS             complement(<1..282)
                     /locus_tag="ACF2SP_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HFD2358592.1"
                     /translation="MHQKKRSSLELEALLSLFEGSLRTIPFNILAVVVLALGFIYTNE
                     VPIKLIIIWFFLILILSVIRWVTSRIIISKEFYTTKGKQSLIGFLLLNFL"
     gene            complement(425..568)
                     /locus_tag="ACF2SP_000791"
     CDS             complement(425..568)
                     /locus_tag="ACF2SP_000791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358593.1"
                     /translation="MEFDYIIVSDGSPGWAFASGLSANPANKLLLLEAGDSVFLEMPN
                     RLQ"
     gene            complement(706..1116)
                     /gene="fur"
                     /locus_tag="ACF2SP_000792"
     CDS             complement(706..1116)
                     /gene="fur"
                     /locus_tag="ACF2SP_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFD2358594.1"
                     /translation="MEESQQLKDAGLKITLPRIKVLQILEQSRNHHLSAEAVYKALLE
                     SGEDVGLATVYRVLTQFEAAGLVSRHNFEGGHSVFELSQGEHHDHLVCVKCGRVEEFV
                     DEIIEQRQKAIAERAHFKMTDHALNIYGICPQCQ"
     gene            1314..1658
                     /locus_tag="ACF2SP_000793"
     CDS             1314..1658
                     /locus_tag="ACF2SP_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFD2358595.1"
                     /translation="MAKFFMVGKKMRIMTFFICFILTLTLTQCASYDFSRRITQQGNL
                     LPQSKIARLKTGMSKNDVAILMGTSLMSPTFNNDRWDYAYTWRRGKGNLEIRNVVLYF
                     SRGSLVKIEHKP"
     gene            complement(1746..2018)
                     /locus_tag="ACF2SP_000794"
     CDS             complement(1746..2018)
                     /locus_tag="ACF2SP_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFD2358596.1"
                     /translation="MVKVELVYIANSKNCLHYKMDLKQGATVEEALIESNIYSTCPET
                     RELPVGVYGKRVSVDQVLKEGDRIEIYRPLILDPKEKRRKLAQFKK"
     gene            complement(2005..2439)
                     /locus_tag="ACF2SP_000795"
     CDS             complement(2005..2439)
                     /locus_tag="ACF2SP_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFD2358597.1"
                     /translation="MTIVKRSRTVPYSCEQMYGLVNDVEHYSEFLPYCAESKILHRDN
                     DEVQATLVIAAAGMSKSFTTRNRLQTNKMIEIRLVDGPFSHLEGFWRFDQEEKGCRIS
                     FDLEFEFAGKIFSMLLGPIFDQITDKMVDAFCERAEVIYGKG"
     gene            complement(2436..3083)
                     /locus_tag="ACF2SP_000796"
     CDS             complement(2436..3083)
                     /locus_tag="ACF2SP_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2358598.1"
                     /translation="MKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFL
                     DAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDTSGIEQSELLIRKVVDAQYNCGFKPHQ
                     IFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
                     PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ"
     gene            3273..3635
                     /locus_tag="ACF2SP_000797"
     CDS             3273..3635
                     /locus_tag="ACF2SP_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358599.1"
                     /translation="MSILFLARKLASDCGFIEQIQQSHNRNQINYRFYSPRTDLHGSN
                     TNRLYGSPGKGVISQNTIFIYWYRDGKNSYFIECSNRLPKKELEVLHQLLMDNQHSLE
                     EILEASNNRFLSFQTRHT"
     gene            3794..3922
                     /locus_tag="ACF2SP_000798"
     CDS             3794..3922
                     /locus_tag="ACF2SP_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFD2358600.1"
                     /translation="MRHIYFALLLIVVTQASLCLSACGQKGPLYLPEQEKKPTTNQ"
     gene            3932..4765
                     /gene="dapF"
                     /locus_tag="ACF2SP_000799"
     CDS             3932..4765
                     /gene="dapF"
                     /locus_tag="ACF2SP_000799"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFD2358601.1"
                     /translation="MGIKFTKMHGLGNDFIVLDGVNQSIQLTVKQIQKLANRHTGIGF
                     DQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATK
                     TTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISIGN
                     PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETI
                     ACGSGAVAAAAIARLFYNLSDKITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLL
                     S"
     gene            5076..7766
                     /locus_tag="ACF2SP_000800"
     CDS             5076..7766
                     /locus_tag="ACF2SP_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0365 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358602.1"
                     /translation="MVDFDKPVSKNDILASLLLIADLNRPSLNQPLSWEQALKSFLSL
                     NDPLIKTEIFIKWLSQLNPDQLQSSPEIVLLLKHIVEQKLLPYTLKKILIQRENFTTS
                     QINWLCNHCLIVAPDQTTLPDIIKLGSWSWLENHMKRNELLNFDLLKIAILNKDYLAS
                     INNSKESQLDFIYALNANKFNYQQILELIALAQDSQIKSLLITCLLSKKEYLDNLKEK
                     SFLAHLNQSELHSPPRLNALIHQLNLSCLTVAQIELLQPEACVTLLCSIPHFHQLSEK
                     QVEKILLKYPKPDMIAYWLNHYSSRPNAHFILAHLMNLVDVHVVDELNKMDSTKKETI
                     IMNMVEHLELFDPTHPLLINQNEEKRLITAIQRYLYGQHHDAYVTYIKLLVYKLLNRN
                     YQFSPQAMQLLICLNNLEEFKELNNKIAYLTNYYLRASAQSGDSSLFYNDSRINITSM
                     TQLVQLAPKIPTKSKEKSLLSSLFCGIKISESSVMTPTNDIPEHPLINKLIKSKRTVK
                     SIDYFLMHYKGDKQVLSKIINDYLSYYVQEGCTEIHRRSIYHLIVLMSLSDISASVRE
                     AIYTAFLDYPELHDEQINFSLFIYDAKRTIRHFGAQASEDSYARVIDLCEGALKKLNP
                     NKHKEIISIATQALTEAKIELNFGENNGFIAQLISRLKRCWYYGWTGFFSPNPPTFVA
                     AIDSPIKQSEKKTSPLLQQRKYFTTKLETNLSIILDDIKSGYTQQKLNELIEALSMFS
                     LQSNPKNELKIRQKLHRLFHFLLLESKNDLTLDAWLIKNQQLLIANRFRLLEMQLMKG
                     ARNEVESLLKEFDEDSTHFSPITKEFTHSLPEQNPYKCESVSSNQTPGATILDSATEL
                     ATSLKSTLGNLGVFFSNNIMSAIYPNNKSTPQSTSTMISP"
     gene            7942..8247
                     /locus_tag="ACF2SP_000801"
     CDS             7942..8247
                     /locus_tag="ACF2SP_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0364 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HFD2358603.1"
                     /translation="MDERRKYFRLKNHGEINASLDNNPIEIVEISSNGAVVVKQKTDI
                     PKEGVLKLQIHNFIMELCYEVIRAEDNNIVLHFTKEDETNKLFLVLKRLRDERKNKT"
     gene            complement(8357..9202)
                     /locus_tag="ACF2SP_000802"
     CDS             complement(8357..9202)
                     /locus_tag="ACF2SP_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFD2358604.1"
                     /translation="MNKLAQQIEALPITLAGRFIYRFLPYRRRLIFSNISQVYNDQLN
                     EYQKKRLAKAYYSHLAKSLKEALQLRFMSEKKLRAQVEVIGHEKMLAVVAQKKGVLVV
                     TGHFGNWEFAPLGGVLNFKEFKGQFHFIRRTLRFKFIERIMFKNYYQAGLNVIPKKNS
                     LEQVCVALEQNHAVIFVLDQHASLVNRDGIAVEFFGKKAGTYRSLATISRHTGIPVIP
                     AASYRLPNGKHVLEFHDPIPWKDYDTTQESLYRNTLAYNQALEKIILAHPEQWNWMHK
                     RWKLS"
     gene            complement(9195..10472)
                     /locus_tag="ACF2SP_000803"
     CDS             complement(9195..10472)
                     /locus_tag="ACF2SP_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFD2358605.1"
                     /translation="MSQANRVFITGRSALTASGATADETWNAIISGQCGIDEIKFWEL
                     SEWSHRLGGEIKDFQPSKMLPDRKLIKVISRQDIMGINAAVQAVEHSKMIAYRDSLAG
                     ADLFNEQTAIYVGSPGNKYFQQYDFIPLLAKTQGDMRSFANHLFDEVHPMWLLRILPN
                     NVLAYTGITYGFKGPNHNVTNHAVGGTQAILEAYHAIRSGQAERAVVVAYDIGTEPQG
                     LFYYEKLGVVSNRHLKPFDKEHDGTILAEGAAALVLESEASVKSRAATCYGEIVGGLS
                     ASEATGLFSIEADGQHLASLMGQTLSTSGINSEEIGLIVTHGNGNSKSDDSEALAIKS
                     VFKQEEVPATAFKWSMGHTLCASGVLDAVLTTYALETKCVPGIANFQQIAPTCEGLSV
                     SREHRELISNSYAMMINRGFASMNVCLVIKACE"
     gene            complement(10473..11765)
                     /locus_tag="ACF2SP_000804"
     CDS             complement(10473..11765)
                     /locus_tag="ACF2SP_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFD2358606.1"
                     /translation="MKRRVAITGLGVVSPLGNDVSTTWNNLIAGHSGIDSIKQFDASA
                     FPTTIAAEVKNFSPDTSISGKHTRFIMSFTHFALEAARQAFEDASIFPTKQTSDRWGI
                     VTGSGMMTAEFEYLTRFQQTCAAGGEIDWGKLQSNSRDFYNLVDFGKTTSNSGLSLLI
                     QQYGITGYASSVHTACASGGQALGLAMQVIRRGEADFMLAGGFDSMINPLGLSSFCLL
                     GALSTYNETPQTASRPFDATRNGFVLGEGAAFLILEEWSKAKARGAKIYAELAGEGNS
                     LSSYRITDSHPNGDGAIQAIKRALEDAGVQPRDVDYINAHGTSTKMNDLSETNAIKVV
                     FGDRIANLPVSSTKSQTGHLIAAAGALEAVLSVKSIEQAQVPKTANLKTPDPECDLDY
                     VVDGPREKSLGVVLSNSFGFGGSNSCLLFKHPEFEEAK"
     gene            complement(11770..12666)
                     /locus_tag="ACF2SP_000805"
     CDS             complement(11770..12666)
                     /locus_tag="ACF2SP_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFD2358607.1"
                     /translation="MRFLFVDRIVQLSPGEWIKGIKHVTRDDAYLTTDDHGRLCFMPS
                     LIGETLGQLAAWNVMQHNHFTSRPVAGVVSSARLYRPAYVGETILLESYIDSLDEKAV
                     QYHSIAKAGNDIIFTIDGALGPMLPMTEFISTEVIRQQFAEIYRPGEWSSVLALSSPV
                     LIEENCSDCGMINSSMQFDRILASEPGISLIAEKRITRSASYFPDHFPNRPVLPMTVL
                     LECKLNLAKEFIARAKYDRAYRISELRKIKMNEFVHPGDVVVCQVKVKQQMDDELILS
                     YRCEVDGKRVCVVDIVLIPKGK"
     gene            complement(12666..13079)
                     /locus_tag="ACF2SP_000806"
     CDS             complement(12666..13079)
                     /locus_tag="ACF2SP_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFD2358608.1"
                     /translation="MNVAEVYPKVREIIADVLVIDEEEISLNSSLIEDLGAESIDFLD
                     LVFQLEKEFKIKIPRGQLEKNARGDLAEDEFEKGGILTTSGLQALKNYLSEVPADRFK
                     ENMKVNEIPVLFTVETFCKLVISAIAQQQATETVA"
     gene            complement(13150..13908)
                     /gene="fabL"
                     /locus_tag="ACF2SP_000807"
     CDS             complement(13150..13908)
                     /gene="fabL"
                     /locus_tag="ACF2SP_000807"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HFD2358609.1"
                     /translation="MSSLVFSGKVGLITGGARGIGKATALKLAQSGCDIAIVYYNSSD
                     EAQSLVNEITGMGRKAVALQADVADCQSVKEMFSQFREHFKHLNFLVSNAASGVLKPA
                     LKMSTKHWRWCLETNALALNHLVTEGLDLMPKNSRVIALSSLGASRAIPNYAFIGASK
                     AALEALVRSLSLELASYGITVNTVSAGVVDTDALKHFPNREQLLDEYQAHALADRALT
                     PEDVANAIYLLCLPEAAMINAHTLYVDAGYSQVG"
     gene            14070..15437
                     /locus_tag="ACF2SP_000808"
     CDS             14070..15437
                     /locus_tag="ACF2SP_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358610.1"
                     /translation="MKYSWNKTVFPIAAIFSFRLLGLFLLIPVFTLYATNLKGATPTL
                     IGLALGSYGLAQGLLQIPFGMLSDKLGRKPMIAIGLLLFAGGSLLGAVTHSIAGMITA
                     RILQGAGAIGSVLIALLADLTLDEQRTKAMAIVGITIGTSFSLAMIISPALTHYYGLS
                     GVFYLTAALAVLGIMILYLIIPNPSKEQFHADSEPSLSLIKPVVTDQQLQRLNFGIFC
                     QHFILTATFFAIPMLLKYQIENGHLHQQWHFYLPLMLFSFILMVPFIIISERKKRLKT
                     VFVISVLLTTFTQGLLVLTSQNWYWFCILMFVYFVSFNVLEATLPSLVSKLASPGSKG
                     TAMGIYSTSQFLGIFFGGSLAGILYQWYDNQAIFLINMILSCLWLIISLSMKSNVYFS
                     TLILPFTANNEESKLVVNQLLKVAGIKEVILSKDENTLYVRVDNEEYSTGSAEKILHQ
                     STIYQ"
     gene            15521..16000
                     /gene="ssb"
                     /locus_tag="ACF2SP_000809"
     CDS             15521..16000
                     /gene="ssb"
                     /locus_tag="ACF2SP_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFD2358611.1"
                     /translation="MARGINKVILVGNVGADPDVRYLPNGNAVTTLSVATSETWKDKT
                     TGEKQDRTEWHRVVCFNRLGEIAGEYIRKGSKLYVEGSLRTRKWQDQQGQDRYTTEIV
                     ASDIQMLDSKGSSATNYDDMPSFQGSSTPQQASTKNQATPTSTAQDAFDELDDDIPF"
     gene            complement(16067..16450)
                     /gene="rplQ"
                     /locus_tag="ACF2SP_000810"
     CDS             complement(16067..16450)
                     /gene="rplQ"
                     /locus_tag="ACF2SP_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFD2358612.1"
                     /translation="MRHRNSGRSFSRTSSHRKAMFSNMCCSLIEHELIRTTLPKAKDL
                     RRYIEPLITVSKSDSVASRRRAFNILRSKSAVGKLFTDLGPRFAKRPGGYIRIIKCGY
                     RDGDNAPMAIVELMDRPVSSDDTEE"
     gene            complement(16469..17461)
                     /gene="rpoA"
                     /locus_tag="ACF2SP_000811"
     CDS             complement(16469..17461)
                     /gene="rpoA"
                     /locus_tag="ACF2SP_000811"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFD2358613.1"
                     /translation="MYTEINEMLTPKVLKVQAESPYKARIVLEPLERGFGHTLGNALR
                     RILLSSMPGSAITEASIDGVLHEYSTIEGVQEDVVDLLLNLKSVAIKLTVGNEAQVTL
                     NKEGPCQVTAGDIQLTHGQEIINPELVIANLNEKGKLNMTLKVERGIGFHNTDAFVRY
                     HDDEIEKKTVGKLKIDNSFSPVKKVAYFVDSARVENRTDLDKLTIELETNGTIDAEEA
                     IRISASILQRQLHAFVDMKFEESRADNKERNDFDPVLLRSVDDLELTVRSANCLKAEN
                     IHYIGDLVQRTESELLKTPNLGKKSLTEIKDVLASRSLSLGMKLENWPPASLGE"
     gene            complement(17480..18100)
                     /gene="rpsD"
                     /locus_tag="ACF2SP_000812"
     CDS             complement(17480..18100)
                     /gene="rpsD"
                     /locus_tag="ACF2SP_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFD2358614.1"
                     /translation="MARYLGPKCKLSRREGCDLLLKSGVRDHKSKCKSEKLPGQHGDK
                     KPRLNSYGIQLREKQKIRRLYGILEKQFRNYYKKAARQKGSTGENLMALLERRLDNVV
                     YRMGFASTRAEARQLVAHKAILVNDKVVNVPSFLVNPGDTISVRQKAKNQGRIQAALA
                     LSEQRAPCDWITVDTGSFKGTFSTAPTLMDLSSDYNVNLVVELYSK"
     gene            complement(18117..18515)
                     /gene="rpsK"
                     /locus_tag="ACF2SP_000813"
     CDS             complement(18117..18515)
                     /gene="rpsK"
                     /locus_tag="ACF2SP_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFD2358615.1"
                     /translation="MAITKSKQQKTRKKVKRVVSDGIVHIHASFNNTIVTFTDRQGNA
                     LCWATSGGSGFRGSRKSTPYAAQVATERAAAVAKEYGMKSVAVFVHGPGPGRESTIRE
                     LITQDFKIVEITDVTGIPHNGCKPPKKRRV"
     gene            complement(18539..18895)
                     /gene="rpsM"
                     /locus_tag="ACF2SP_000814"
     CDS             complement(18539..18895)
                     /gene="rpsM"
                     /locus_tag="ACF2SP_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFD2358616.1"
                     /translation="MARIAGVNIPDHKHVVIALTAIYGIGKTTSLKLCKTVDIDPSVK
                     VSQLSDAQLESLRTEIAKITVEGDLRRVVTMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     KTNARTRKGRRKGTSS"
     gene            complement(18973..19086)
                     /gene="rpmJ"
                     /locus_tag="ACF2SP_000815"
     CDS             complement(18973..19086)
                     /gene="rpmJ"
                     /locus_tag="ACF2SP_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFD2358617.1"
                     /translation="MKVRASVKRICRNCKIIKRSGTIRVICKDARHKQKQG"
     gene            complement(19103..20431)
                     /gene="secY"
                     /locus_tag="ACF2SP_000816"
     CDS             complement(19103..20431)
                     /gene="secY"
                     /locus_tag="ACF2SP_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFD2358618.1"
                     /translation="MKNQKHNGSKPRGGLAELKSRLLFVVLGILVYRLGAHIPVPGLD
                     PAKLANFFSEQQNTIFGLFNMFSGGALSRVTVFAIGIMPYISASIIIQLFSVVSPKLE
                     QLKKEGESGRRKINQYTRYLTLLLSIFQSMGMARWLASQQIAMQPDFSFYFTAVVTLV
                     TGTMFLMWLGEQITEKGVGNGISLIIFSGIVSSMPNAIGSVLQQVKEGQMQALTLVII
                     AAVVIVVTGFVVFMERAQRRIRVNYAQRTQGRKVYAAQTSHLPLKINMSGVIPPIFAS
                     SIILLPATLAQFFSQTKGLGWLSDVGMALSPGQPLYLIVYAAAILFFAFFYAALVFNP
                     KDTADNLKKSGAYIPGIRPGEQTTKYIDSVMTRLTLVGAIYLVLVCLLPQILMYTWHV
                     PFYFGGTSLLIIVVVIMDFVAQVQAHLMTQQYDSLLKKANFRGTKLPGLL"
     gene            complement(20434..20868)
                     /gene="rplO"
                     /locus_tag="ACF2SP_000817"
     CDS             complement(20434..20868)
                     /gene="rplO"
                     /locus_tag="ACF2SP_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFD2358619.1"
                     /translation="MNLNTLSPDPGSRPSRRRVGRGIGSGLGKTCGKGHKGQKSRAGG
                     YHKINFEGGQMPIQRRLPKMGFKSRVGRTIDEVSLGELAKLNDEVIDLVALRKAGLIN
                     NSIKDVKVILSGELTAAIKLKGLRVTKGARSAIESLGGSIEE"
     gene            complement(20868..21053)
                     /gene="rpmD"
                     /locus_tag="ACF2SP_000818"
     CDS             complement(20868..21053)
                     /gene="rpmD"
                     /locus_tag="ACF2SP_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFD2358620.1"
                     /translation="MGKKIKITLVKSTIGRKPKHVAIAKQLGLGKTNSSVVHSDTPAI
                     RGLVNEINYLLLVEESA"
     gene            complement(21056..21562)
                     /gene="rpsE"
                     /locus_tag="ACF2SP_000819"
     CDS             complement(21056..21562)
                     /gene="rpsE"
                     /locus_tag="ACF2SP_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFD2358621.1"
                     /translation="MSFDELPKSDGYQEKLVSVTRTAKVVKGGRVFGFAVLVVVGDGK
                     GKVGFGRGKAREVPIAIQKAMDQAKKNMVYIPLSGTTIFHEITWNYGASKVFMKPASE
                     GTGIIAGGAMRAVLEVLGVQNILAKSIGSTNPSNIVRATIGALTHIGTPDYVAAKRGK
                     TVEEVMAG"
     gene            complement(21572..21931)
                     /gene="rplR"
                     /locus_tag="ACF2SP_000820"
     CDS             complement(21572..21931)
                     /gene="rplR"
                     /locus_tag="ACF2SP_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFD2358622.1"
                     /translation="MNKQNARHRRGLKAKALIRKTGRSRLVVYRSGVHIYSQIVKSDQ
                     LGDKVLVASSTIDKELRSSLTGKSKVEQASLVGQLLGKRAKAAGITQVAFDRAGYKYH
                     GRVKALAEGAREAGLDF"
     gene            complement(21942..22481)
                     /gene="rplF"
                     /locus_tag="ACF2SP_000821"
     CDS             complement(21942..22481)
                     /gene="rplF"
                     /locus_tag="ACF2SP_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFD2358623.1"
                     /translation="MSRVAKAPVIHSANVEVTFVDGVITVKGPKGILTQKINKLVNIQ
                     HSKESNKLEFSPASNDPMGWAQAGTARALVRNMVQGVTEGYTVTLELVGVGYRAQSKD
                     KSISLSLGYSHSIEYDLPKGVTVETPNNTTILLKGVDKQVLGQIASEIRAFRPPEPYK
                     GKGVKYAGEQIVRKEAKKK"
     gene            complement(22499..22894)
                     /gene="rpsH"
                     /locus_tag="ACF2SP_000822"
     CDS             complement(22499..22894)
                     /gene="rpsH"
                     /locus_tag="ACF2SP_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFD2358624.1"
                     /translation="MSMHDPIADMLTRIRNGQQAKHQQVTLVSSKLKEEIARVLKEEG
                     YIQDFFTETLPNGLKSITLKLKYYHGRPVIEFIKRISRPGLRVYKSYKDLHSIPGFGV
                     AILSTSKGIMTHVSAKVKGVGGEVICEVA"
     gene            complement(22917..23219)
                     /gene="rpsN"
                     /locus_tag="ACF2SP_000823"
     CDS             complement(22917..23219)
                     /gene="rpsN"
                     /locus_tag="ACF2SP_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFD2358625.1"
                     /translation="MAKKSMLMRESKRAKLVEKYRQRRNELKQLIKSSDDFQVIMESQ
                     AKLAKLPVNSNPVRYVTRCKQCGRPHAVYRKFNLCRICLRQQLMVGNIPGGRKSSW"
     gene            complement(23232..23783)
                     /gene="rplE"
                     /locus_tag="ACF2SP_000824"
     CDS             complement(23232..23783)
                     /gene="rplE"
                     /locus_tag="ACF2SP_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFD2358626.1"
                     /translation="MARLKEFYKKDVVTMMMKRFNYSSVMEVPRILKITLNMGVGEAV
                     GDKKVMNHAIEDMTLISGQKPVVTKARKSIAGFKIREGWPIGCKVTLRRERMYEFLDR
                     LISITLPRVRDFRGLNPKSFDGTGNYSMGIHEQIVFPEIDYDKTDGIRGLDICITTSA
                     KTNEEAKALLEAFNLPLKDKDRK"
     gene            complement(23799..24128)
                     /gene="rplX"
                     /locus_tag="ACF2SP_000825"
     CDS             complement(23799..24128)
                     /gene="rplX"
                     /locus_tag="ACF2SP_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFD2358627.1"
                     /translation="MKRIKSGDEVIVIAGKSKGHIGKVLRVVDDAVVVEGGNLIKKHI
                     KPNPQKPENKGGIIAREAPLHVSNVAHYNPVTKKADKVGFKYLESNGVSKKVRYYKSN
                     NEIIDRI"
     gene            complement(24141..24506)
                     /gene="rplN"
                     /locus_tag="ACF2SP_000826"
     CDS             complement(24141..24506)
                     /gene="rplN"
                     /locus_tag="ACF2SP_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFD2358628.1"
                     /translation="MIQMQTVLEVADNSGARKVMCIKVLGGSHRRYARVGDVIKVSVK
                     DAIPRSKVKKGAVMRAVVVRTAQGVRRDDGSLIRFDDNAAVLLNNQNEPIGTRIFGPV
                     TRELRERFMKIISLAAEVL"
     gene            complement(24595..24849)
                     /gene="rpsQ"
                     /locus_tag="ACF2SP_000827"
     CDS             complement(24595..24849)
                     /gene="rpsQ"
                     /locus_tag="ACF2SP_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFD2358629.1"
                     /translation="MSTNSESNARTMIGKVVSDKMDKTIVVMIERTVKHPKYGKIMKR
                     RTKLHAHDENQVCRVGNTVKIRESRPLSKTKSWVLVEVIS"
     gene            complement(24851..25045)
                     /gene="rpmC"
                     /locus_tag="ACF2SP_000828"
     CDS             complement(24851..25045)
                     /gene="rpmC"
                     /locus_tag="ACF2SP_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFD2358630.1"
                     /translation="MKKIDELRNMSVEELQNELLSLRKDQFNLRMKKASGSLDKTHLI
                     TMVRKSVAKVKTILTEKAGK"
     gene            complement(25045..25458)
                     /gene="rplP"
                     /locus_tag="ACF2SP_000829"
     CDS             complement(25045..25458)
                     /gene="rplP"
                     /locus_tag="ACF2SP_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFD2358631.1"
                     /translation="MLQPKRTKYRKQMKGRNRGLALRGSKISFGEFGLKAVERGRLTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDKPITQKPLEVRQGKGKGSVEYWVAQIQPGKV
                     LFEMEGVSKELAMEAFDLAKAKLPFKVMFEERTVM"
     gene            complement(25475..26131)
                     /gene="rpsC"
                     /locus_tag="ACF2SP_000830"
     CDS             complement(25475..26131)
                     /gene="rpsC"
                     /locus_tag="ACF2SP_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFD2358632.1"
                     /translation="MGQKVNPIGIRLGIIKDWNSKWFAGKRYAEFLIQDIKLRNDLKK
                     KLMAAAVSKILIERPANNAVVTILTARPGVIIGKKGGGIETLRKEISDNLGVPVHLNI
                     EEVKKPELDATLVAEGIAQQLEQRVMFRRAMKRAVTSALKAGAKGIKICVSGRLGGAE
                     IARSEWYREGRVPLHTFRADIDYGTAESKTTYGIIGVKVWIFKGEILPQKKRSTESAQ
                     "
     gene            complement(26134..26469)
                     /gene="rplV"
                     /locus_tag="ACF2SP_000831"
     CDS             complement(26134..26469)
                     /gene="rplV"
                     /locus_tag="ACF2SP_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFD2358633.1"
                     /translation="MEVTAKLKGAPLSAQKGRLVADMIRNMNVSGALDVLKFTPKKGA
                     KLMLKLLESAIANAENNNGADIDDLKVGMVCVDEATTLKRISPRAKGRANRICKRTCH
                     ITIKVSDEE"
     gene            complement(26479..26757)
                     /gene="rpsS"
                     /locus_tag="ACF2SP_000832"
     CDS             complement(26479..26757)
                     /gene="rpsS"
                     /locus_tag="ACF2SP_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFD2358634.1"
                     /translation="MARSIRKGPFIDHHLISKVEAAIESKSKKPIKTWSRRSTIVPEM
                     IDLTIAVHNGKDHVPVFITDNMVGHKLGEFAMTRTFKGHSGDRKAKGK"
     gene            complement(26776..27603)
                     /gene="rplB"
                     /locus_tag="ACF2SP_000833"
     CDS             complement(26776..27603)
                     /gene="rplB"
                     /locus_tag="ACF2SP_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFD2358635.1"
                     /translation="MALLKSKPTSPGKRGEIRVVHHDIYKGKPHAALVEKLKKTGGRN
                     NQGRITVRHIGGGQRQKYRIIDFKRNKDGILGRVERLEYDPNRTALIALISYKDGEKR
                     YIIAPSNLEVGATIQSGADSPISVGNCLPLKNIPVGTTIHCVEMKPGKGAQMLRSAGC
                     SGQLVAKEGVYATLRLRSGEMRKIHVLCRAVIGEVSNSEHNLRALGKAGAKRWRGIRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGLPTKGYKTRSNKRTDTFIVRGRKKK"
     gene            complement(27615..27911)
                     /gene="rplW"
                     /locus_tag="ACF2SP_000834"
     CDS             complement(27615..27911)
                     /gene="rplW"
                     /locus_tag="ACF2SP_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFD2358636.1"
                     /translation="MNAERLMMVLREPHTSEKATVMADKFKQFTFKVLKNATKTEIKL
                     AVEHIFNVKVKSVSVVNVKGKSKRFKQTSGKRSDWKKAFVSLHADQDIDFTVTE"
     gene            complement(27908..28513)
                     /gene="rplD"
                     /locus_tag="ACF2SP_000835"
     CDS             complement(27908..28513)
                     /gene="rplD"
                     /locus_tag="ACF2SP_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFD2358637.1"
                     /translation="MEITTIDTKSKLKLNKEIFAYTYNEGLVHQAVVTFMNNARSGNS
                     AQKTRSEVSGGGKKPWNQKGTGRARAGTIRSPLWRSGGVTFASKKRDYSQKLNKKMYK
                     RALRSIISELCRTGNLVVVSDFQCDNHKTKDFLKKMNQMEISNALIIMSEVGENEYLG
                     SRNLIDYDICDVTTIDPVSLLRFEKVVVTEAAIKKIEEQLQ"
     gene            complement(28513..29160)
                     /gene="rplC"
                     /locus_tag="ACF2SP_000836"
     CDS             complement(28513..29160)
                     /gene="rplC"
                     /locus_tag="ACF2SP_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFD2358638.1"
                     /translation="MIGLLGRKIGMTRVFTPEGVSVPVSVVEVQPNRVSQVKTAANDG
                     YSAVQLTGGTKKSSKVSKPVAGHFAKAQIDAGDMQVEFRIDSEDAFTPGQVISVADVF
                     TAGQYVDVSGLTKGKGFAGTVKRHNFRTQDASHGNSRSHRVPGSIGQNQTPGRVFKGK
                     KMAGHMGNARCTIQSLELVKVDSERNLLLIKGAIPGAPGSRVEIKPAVKKQARGE"
     gene            complement(29198..29515)
                     /gene="rpsJ"
                     /locus_tag="ACF2SP_000837"
     CDS             complement(29198..29515)
                     /gene="rpsJ"
                     /locus_tag="ACF2SP_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFD2358639.1"
                     /translation="MSSNQNIKIRLKSFDHRLIDLSTREIVDTAKRTGAQIRGPIPLP
                     IRKEKFTVLTSPHVNKDARDQYELRTHKRLVVIVHPTEKTVDALMKLDLAAGVDVQIS
                     LDD"
BASE COUNT         8943 a         6408 c         4813 g         9353 t
ORIGIN      
        1 taaaaaattc agcagtaaaa aaccaattaa agactgcttt ccttttgtgg tgtaaaactc
       61 tttactgatt ataattcgac tagttaccca acgaatcaca cttaaaatta aaatgagaaa
      121 aaaccagata ataatcagtt tgattggaac ctcgttcgta tagataaaac ccaaagcaag
      181 taccaccact gccaggatat tgaatgggat agttcttaag gatccctcaa ataatgacag
      241 taaggcttca agttccaatg aagaccgctt cttttgatgc ataattattc aaaacccaag
      301 caccttcatt ataatttcaa gtatagacta gtttagagag ttactcttta ccacccaaat
      361 tccatttaga ataggaggct ctcacgaaga attggaatat agagtgataa ttgttctgcg
      421 attctcattg caggcggttt ggcatttcca gaaatacgct atctcccgct tccagcaaca
      481 ataatttatt ggcgggattg gcacttaacc cggaagcaaa agcccatcct gggctaccat
      541 cactaacaat aatataatca aactccatgt aatgaatcct taaagaaaac agcgttttac
      601 caaaacactc tgattattgt aataataaag cactgcaaag gaatatagta aatacgctta
      661 gaatattccg tgaggtgaca agccaatcca gttaaaatat caaagtcatt gacactgcgg
      721 acaaatacca tatatgttta gcgcatgatc tgtcatttta aaatgagcgc gctcggcaat
      781 tgctttttgt ctttgttcta ttatttcatc tacaaattcc tctacccgcc cacacttaac
      841 acaaacaaga tggtcatgat gttcgccctg agacaactca aacaccgagt gtcctccttc
      901 aaaattatgc ctcgatacta atcccgctgc ttcaaattga gtaagaactc tatatacagt
      961 agctaaccca acatcctcac ccgattccaa taaggcttta tagactgctt ctgcactcaa
     1021 atgatggttt cttgattgct ccaatatttg caataccttg atacgaggta atgtgatttt
     1081 taatccagcg tcttttaact gttgactctc ttccacttgt gctccttaac tgtaacgaat
     1141 ttcgagcttt gatcattatt ccagcaaaat tggattaata attagcatca aggcctaaag
     1201 taattttcat gctctaaaaa ccattagcgg ccttaaaact tagtctggcc gaataatggc
     1261 aaataccaaa ttccacatga tgacaggtgc ctttcaataa atgacatgtt attatggcga
     1321 aatttttcat ggtaggcaaa aaaatgcgaa taatgacttt ttttatttgt ttcattttaa
     1381 cattaacctt aacccaatgt gcatcatatg acttctcaag gcgcatcact cagcaaggta
     1441 atttattgcc tcaatccaaa attgcccggt taaaaacagg catgagtaag aatgacgttg
     1501 caatcttgat gggtaccagc ttaatgagtc caacattcaa caatgatagg tgggattatg
     1561 cttatacctg gcgacgagga aaaggtaatc tcgaaattcg taatgttgtg ttatattttt
     1621 ccagaggttc tttagtaaaa atagaacata agccgtaatt taatatctcc cctttgccta
     1681 agtgattaga caaggggaaa gttgtttgta agagttcagt gtagattaaa atgcctaata
     1741 tgcctctatt ttttaaactg cgctaactta cggcgttttt ctttggggtc gagtatcaat
     1801 ggtctataaa tctcaattcg atctccttct ttcaatacct ggtccacaga aactcgcttc
     1861 ccgtatactc ctacaggtaa ttccctcgtt tcaggacaag ttgaataaat atttgattca
     1921 atgagtgcct cttcaactgt agctccctgt tttaaatcca ttttgtaatg caggcaattt
     1981 ttagagttcg ctatatagac taattcaacc tttaccataa atgacctcgg ctctttcgca
     2041 aaatgcgtca accattttat cggttatctg atcaaatata ggaccaagaa gcatggaaaa
     2101 tattttcccg gcaaactcaa actccaaatc aaaagaaatt ctgcaccctt tttcttcctg
     2161 atcaaaacgc caaaatccct ctaaatgact aaaaggacca tcaaccaaac gaatttcaat
     2221 cattttatta gtttgcaatc gattacgagt agtgaatgac ttactcattc cagcagctgc
     2281 tatcaccaaa gtcgcctgga cttcatcatt gtcacgatga agtattttgc tttccgcaca
     2341 ataaggtaga aactcggaat aatgttcaac atcattaacc agtccataca tttgctcaca
     2401 agaataaggt actgttcttg acctttttac tattgtcatt gtaaaactcc ttgaacctgc
     2461 ttattaagcc aaaggctaag atctttaatt tcttcaaaac atatggaatg ttccatgggg
     2521 tattgatgaa aagagacttc attataccca ttagccaaga gccaatcctt actctgtaga
     2581 gtccattttg gcagaactaa aggatcaaat tgtcctgctc ccataaagat aggagtattc
     2641 ttatcaagtt gcggcttatt atgcttagcc aagggcaaat aagcggataa tgcaataacg
     2701 ccgcataatc gctcagtcat atgaagagcc gtgtgcaatg ccatagctcc cccttgagaa
     2761 aagccagcta aaaaaatttg atgaggttta aagccacaat tatattgagc gtcaaccacc
     2821 ttgcgtatta acaattcaga ttgctcaatg ccagacgtat cttcttcatc gacaaagcct
     2881 aatccataaa tatcatacca cgcaggcata accattcctc cattcagagt cactggtctt
     2941 cgaggagcat caagaaatac atgccttaaa gcggtatctt ctattgttag ctgatcagct
     3001 aaacccatca tatcagaggc atcagctccc aacccatgca tccatatgac acaagcttgt
     3061 gctttctcta aaggttcctt catataaacg ctcaaaacaa attctccacg caaaaacgta
     3121 aattatcgcc aatgcaatag agcagcgcaa gtttaacatt taaaaatata ttgaacaatt
     3181 tataggcata ttgacactgg taattctaat aactattgac tttattgtat aaatctttat
     3241 cattgtgttt attttgcatg cttttctgtt ttatgagtat tttgttttta gcaagaaaac
     3301 tggcttctga ctgcggattc attgagcaaa tccagcaaag ccataatcgt aaccaaataa
     3361 attatcgctt ttattccccc cgaactgatc tgcatggttc caatacaaac aggttatatg
     3421 gaagtcctgg caaaggtgtc atttcacaaa atacgatttt catatactgg tacagagatg
     3481 gaaaaaacag ttattttatt gaatgttcaa atagactacc taaaaaggaa cttgaagtgt
     3541 tgcatcaatt gctcatggat aatcaacact ctttggaaga gatactcgag gcatccaata
     3601 atagatttct ctcttttcag acacgccata cctaagcact acttcactaa agaaagacac
     3661 aaaattcgta agaagctatc tgcctgtctt actcaagctt aaaatattcc catataaaat
     3721 aacaagaaga atgaatcatt gagtttcaaa gcaaatctca tataattaaa atttaactct
     3781 ataataatac ctcatgagac acatttattt cgcattatta ctcatcgttg tcacccaggc
     3841 ttctttgtgt ttgtcagctt gtgggcaaaa aggacctttg tatttgcctg aacaagaaaa
     3901 gaaaccaaca accaatcaat agagacatcc tatgggaata aaatttacca aaatgcatgg
     3961 tttaggcaat gacttcattg tattggatgg agtcaaccaa tctattcaac tcactgttaa
     4021 gcaaatacaa aaactagcca atcgccatac cggtataggg tttgaccaat gtttactaat
     4081 agaatccagt caaaccgaag gaattgattt taattatcga atcttcaacg ccgatgggca
     4141 agaagtaggt caatgtggta atggggcgcg atgcatagct ctttttgcca gatattatgg
     4201 tttaacagca aaaaataaat tgacagtagc aactaaaact acgctcatgg atttaatcat
     4261 caatgaggat aacagcgtga gtgtcaatat gggcgttccc agactagccc caggtgaaat
     4321 tcctttactt gctgatcgcc aatccccaga atattcactt gaattaaata acggcaatac
     4381 agtgaatctt catgcgataa gtataggcaa tcctcatgct gtattgttgg ttgaaaatat
     4441 agatacagcc cctgtaaaca gtttgggaca acagattagt tttcacccgc aattcccaga
     4501 gcaagtcaac gttggattta tgcaaattgt taatcatgaa aaaatcaact tacgtgtcta
     4561 cgaaaggggt tgtggtgaaa cgattgcttg tggtagtggt gcggttgctg cggcagcgat
     4621 agcacgttta ttttacaacc tgtcagacaa aatcaccgtt catttgccag gaggtgattt
     4681 gtgtatccaa tggccttgtc ccactgctcc cattatatta acagggcctg ctgcttttgt
     4741 gtatgagggc acattacttt cataacaaaa ttcacaaaat taaaacaagt attgaatttt
     4801 tttttcaaac agttattctg aattaatcca caaatataaa acaggactta ggaaaaacgc
     4861 taaggtcaag gcaaaaaaat ttttaatgag ggagtttaga taaactaaat ggccgaatta
     4921 aaaactggtt tttacgaaaa gattcggggg ttgcttaagt cctgtaaaga ttccatcggg
     4981 agaatttgtg aatattatat tccgtggctt aacatgaacg aaatgggtat taaagttgat
     5041 agagtagttt tggcatttcg gtcaaggagg atcaagtggt tgattttgat aaacctgtct
     5101 caaaaaatga tattcttgcc agcttattac ttatcgcaga tttaaataga ccatctctaa
     5161 atcaacctct ttcttgggag caagctctaa aatcattttt atcgcttaat gatccactga
     5221 ttaaaacgga aatatttata aaatggctca gtcaattaaa tcctgatcaa cttcaatcgt
     5281 ccccagagat agtcctcctt ttgaaacaca ttgtcgagca aaagctgctc ccctacaccc
     5341 tcaaaaaaat attgattcag cgtgaaaact tcaccacctc gcaaatcaat tggctttgca
     5401 atcattgtct tatagttgca cctgatcaaa cgactttacc tgatattatt aaactcggct
     5461 catggagctg gctggagaat catatgaaaa gaaatgaact attaaatttt gatttgttaa
     5521 aaatagccat cttaaataag gattatttag ccagcatcaa caatagtaaa gaaagtcagc
     5581 ttgattttat ctatgcgtta aacgctaata aatttaacta ccaacaaata cttgaattaa
     5641 ttgctttagc gcaggatagc cagattaaat cattattaat tacctgtcta ttaagcaaaa
     5701 aggagtacct ggacaattta aaagaaaaat catttctagc ccatttaaat caaagcgaac
     5761 ttcactcgcc acctcggctt aatgcactaa ttcaccaatt aaacctgagt tgtttgactg
     5821 tggcacaaat agaacttcta caacctgaag catgtgttac tttactctgt tcgatccctc
     5881 attttcatca attgtcagaa aaacaggttg aaaaaatact acttaaatat ccaaagcctg
     5941 atatgattgc ctattggcta aatcattact catccaggcc aaatgcgcat tttattttag
     6001 cacatttgat gaacctggta gatgttcatg ttgttgacga acttaataaa atggattcca
     6061 caaaaaagga aaccattatt atgaatatgg tggaacatct cgaattattt gatccgactc
     6121 atcctctttt gatcaaccaa aatgaagaaa aacgattaat taccgctatt caacgctatc
     6181 tctatggcca acatcatgac gcgtatgtaa cttacatcaa gttattggtt tataaattat
     6241 taaaccgtaa ttaccaattc tcacctcaag cgatgcaact cctgatttgt ttaaataatc
     6301 tggaagagtt taaagagtta aataataaaa tcgcctatct gaccaattac tacttgcgag
     6361 catcagcaca atccggtgat tcgagcttat tttataatga ttcacgcata aacattacga
     6421 gcatgacaca actggtccag ctagccccaa aaattccaac taaatcaaaa gaaaaaagtc
     6481 ttttaagcag cctcttctgc ggcatcaaaa ttagcgaaag ctctgtaatg acaccgacca
     6541 atgatatacc tgaacaccct ttaattaata aattaataaa aagcaagaga acggtgaaat
     6601 cgatagatta ttttttaatg cattataaag gcgacaagca agtactttcc aaaatcatca
     6661 atgattattt aagttactat gttcaagaag ggtgcactga aatacatcgg cgttcgatat
     6721 atcatttaat tgttctcatg tctttaagcg atatatcagc ttctgttaga gaagctatat
     6781 acacagcgtt tctagactat cctgaattac atgatgagca aattaatttt tctttattta
     6841 tctatgatgc taaaagaact atccggcatt ttggggcaca agctagcgaa gacagctatg
     6901 ccagagttat tgatttatgt gagggagctc taaaaaaatt aaatccaaat aaacacaagg
     6961 aaattatcag tatagcgact caagctttaa ctgaagcaaa aatcgagtta aattttggtg
     7021 aaaataacgg atttatcgct caactgattt cacgattgaa acgatgttgg tattatggct
     7081 ggactggttt tttttcccca aatccaccta cttttgttgc agcaattgac tcgccaataa
     7141 agcaaagcga aaaaaaaacc tcccctcttc ttcaacaaag aaaatatttc actaccaaac
     7201 ttgaaacaaa tctgtcgatt atcctcgatg acataaaatc aggttacacc caacaaaaac
     7261 ttaatgaatt aatcgaagca ttgagtatgt tttcattgca atccaatcca aaaaacgagc
     7321 ttaaaattcg tcaaaaactc catcgcttat ttcactttct gttactagaa agcaaaaatg
     7381 acttaacatt ggatgcctgg cttataaaaa atcagcaact tttaatcgcg aatcgttttc
     7441 gtttgttaga aatgcaacta atgaaagggg ctcgtaatga agttgagtct ttgcttaagg
     7501 aattcgatga agactctact catttcagcc ctatcactaa agagtttact cattctttac
     7561 ccgaacaaaa tccgtataag tgtgaatctg tttcttcaaa tcaaactcca ggcgcaacta
     7621 tcctggattc agcaactgag ttagctacta gcctaaaaag cacattaggc aacttgggag
     7681 tattttttag caacaacatc atgtcagcta tttatccaaa taataaatcc actccccaat
     7741 cgacatcaac aatgatttca ccttgattaa tcttccggct gctcatctac agccggattg
     7801 attctataat aacctgaaac aaacaaccgt tctaaaattt ggacccttcc tatttaatca
     7861 gcagatatca cgctcatccg gttgacctat caggaataat agacctaaaa ttaaaaagca
     7921 cacttaattt atggttaaac aatggacgaa aggcgaaaat attttcggct aaaaaaccat
     7981 ggcgaaataa atgccagctt agacaataat ccaattgaga ttgttgaaat ctcttccaat
     8041 ggggctgtag tagttaagca gaaaactgat attcctaaag agggagtgct taaactacaa
     8101 atccataact tcattatgga actttgctat gaagtgattc gagctgaaga caataacatt
     8161 gtccttcatt tcacaaaaga agatgaaacg aataagttat ttctggtact caaacgatta
     8221 agagacgaac gaaaaaataa aacataaaaa tttgacataa cttgcctcca taaaaagtca
     8281 tttgtgatta agtaggagct tagagaaaac attctgtgac aaagaataaa taagaaaccg
     8341 aagacaattc attcatttaa gaaagtttcc agcgtttatg catccaattc cactgctccg
     8401 gatgtgccag aataatcttc tccaaagcct gattataagc caaggtattt cggtataaag
     8461 attcttgcgt agtgtcataa tctttccaag gaatgggatc atgaaactcc aaaacatgct
     8521 ttccattagg taatctgtag ctagctgcag gtataactgg aattcctgta tggcgagata
     8581 tcgtcgccag gcttctgtag gtgccagctt tttttccaaa aaactctacc gctatcccgt
     8641 ctctgttcac cagggatgca tgctgatcca atacgaagat tacggcatga ttttgttcta
     8701 acgcaacaca aacctgctcc agagagtttt ttttaggaat aacatttagg ccagcttgat
     8761 agtaattttt gaacataatg cgttctatga acttaaatcg caaggtacgc ctgatgaaat
     8821 gaaattggcc tttgaattcc ttgaagttta atacccctcc caagggcgca aactcccaat
     8881 ttccaaagtg accggtaact actaacactc cttttttctg tgccacaaca gctaacattt
     8941 tttcatggcc gattacttca acctgagctc gtaatttttt ttcactcatg aaccttaatt
     9001 gcagcgcttc ttttaaagat tttgcaagat gtgagtaata cgcttttgcc aagcgctttt
     9061 tttggtattc attcagttga tcattataaa cctggctaat attggaaaaa atcaaccggc
     9121 gcctgtatgg caaaaaccga taaataaaac gtcccgccag tgtaatgggt aatgcttcta
     9181 tttgttgagc taatttattc acaagctttg atcaccaaac atacattcat actggcaaac
     9241 cctcggttga tcatcatggc ataggaatta gatattagct ctctatgctc acgacttact
     9301 gataagcctt cacacgttgg ggcaatttgt tgaaaattag cgatccccgg cacacatttg
     9361 gtttcaaggg catatgtagt taataccgca tccaatactc cagaagcgca taaagtatgt
     9421 cccatggacc atttaaacgc agtcgcagga acttcttctt gtttgaatac tgatttgata
     9481 gccagggctt cactgtcatc tgatttactg tttccatttc catgggttac tattaaccct
     9541 atttcctcag agtttattcc tgatgtgctt aatgtctgcc ccatcaatga agccaaatgt
     9601 tgcccatctg cttcaataga aaataatcct gttgcttcag aagcggataa accaccaact
     9661 atctcgccat aacaagtcgc agccctggac ttcacactgg cctcactttc caatacaagg
     9721 gccgcggcac cttcagcaag aatagtaccg tcatgctctt tatcaaaagg ttttaaatga
     9781 cgattactta ccacacccaa tttttcataa taaaataagc cttgaggttc tgtgcctata
     9841 tcataggcaa ccacaaccgc gcgttcagcc tgcccgctac gtatggcatg atacgcttcc
     9901 aaaatggctt gtgtgccacc taccgcatga tttgttacat tgtgatttgg gcctttaaag
     9961 ccataggtaa tacctgtata ggcaagcaca ttattgggta aaatcctcaa taaccacata
    10021 ggatgcactt catcaaacaa gtggtttgca aagctacgca tatctccctg ggttttggcc
    10081 aacaaaggta taaagtcata ttgttgaaaa tacttattcc ctggagaacc aacataaatg
    10141 gcggtttgtt cattaaataa atcagcacct gccaggctat ctctgtaagc aatcatttta
    10201 ctgtgctcaa cagcttgcac tgccgcattg attcccatta tatcctggcg ggaaataact
    10261 tttattaatt ttctatcagg aagcatcttg gaaggctgga aatcttttat ctctccaccc
    10321 aaacgatgcg accattcaga taattcccaa aatttaattt catctattcc acactggcct
    10381 gaaatgattg cattccaagt ttcatcagca gttgcaccag aggcagttag agcgctgcga
    10441 ccagtaataa ataccctatt cgcttgactc atttattttg cctcctcgaa ttctggatgt
    10501 ttaaataata agcagctatt agaccctcca aaaccaaacg aattagataa tactacccct
    10561 aaagattttt cacgtgggcc atcaacaaca taatccaaat cgcactccgg atcaggcgtt
    10621 tttaaattcg ctgttttggg aacttgcgcc tgttctatgg atttaactga caataccgct
    10681 tcaagtgctc ctgcagctgc gattaaatgc cccgtttggc ttttggtaga gctaacaggt
    10741 aaattagcga tcctatcccc aaagacaact ttgattgcat tggtttcact taaatcgttc
    10801 attttagtgg atgtgccatg cgcattaata taatcgacat ctcgtggttg aacaccggca
    10861 tcctccaacg cacgtttaat cgcttgaata gcaccatcac cgttgggatg tgaatcagtt
    10921 attcggtagg agctcaaaga attcccctct ccggccagtt cagcatagat ttttgcccca
    10981 cgggcttttg ctttactcca ttcttcaagg atcaaaaagg cagccccttc acccaggaca
    11041 aaaccattgc gtgtcgcatc aaaggggcga cttgccgttt gcggagtctc attataagtt
    11101 gataaggcac ccaaaagaca aaagctcgac aaacccaaag gattaatcat ggaatcaaat
    11161 ccacctgcca gcataaaatc tgcctcgcca cgacgaatca cctgcattgc caatcccaag
    11221 gcttggcctc ctgaagcgca ggccgtatgg acagaagagg cataaccagt aatcccatat
    11281 tgttgtatta ataaggaaag accagagttt gaagtggttt taccaaaatc aaccaaatta
    11341 tagaaatccc tgctattaga ttgcagcttt ccccaatcaa tttcaccgcc tgcagcacaa
    11401 gtttgttgaa accgcgttaa atactcaaac tcggccgtca tcatcccact gcctgttacg
    11461 attccccacc gatcggatgt ttgcttggta gggaagatag aagcatcctc aaaagcctgg
    11521 cgtgcagctt ccaatgcaaa atgagtaaaa gacatgataa atcgagtatg ctttcccgaa
    11581 atagacgtat caggtgaaaa attctttact tcagcagcaa tagtggtagg aaaagcactg
    11641 gcatcaaact gcttgatact atctatgcca gaatggccag cgatcaaatt gttccaagtg
    11701 gtggatacat catttcctag aggcgaaaca acccctagac ctgtaatagc aactcgtcgt
    11761 ttcataacct catttacctt ttgggataag cactatgtcg acaacacata cccgcttgcc
    11821 atcaacctca cacctataag acagtattaa ttcatcatcc atttgctgtt tcactttgac
    11881 ctggcaaact acgacatctc cgggatgaac aaattcattc attttaattt tacgtaattc
    11941 gcttattcga taagctctat catattttgc tctagctata aactctttcg ccaaattcaa
    12001 tttacattca agaagtaccg tcatcggtag tacaggtctg ttaggaaaat gatccgggaa
    12061 ataagatgct gaacgagtta ttcttttttc agcaatcaag cttatacctg gttcactggc
    12121 gaggattcta tcaaattgca tggaagaatt aatcattcca caatcagagc agttttcctc
    12181 tattaaaaca ggcgaactca aagctaacac agaagaccac tcacctggcc ggtatatctc
    12241 tgcaaactgc tgtctaatga cttctgtact aataaattca gtcataggca gcatggggcc
    12301 taatgcccca tctatagtaa aaataatatc attacctgct ttggcaatgc tatggtattg
    12361 aaccgccttt tcatccagtg aatcaatata tgattcaagc aatatcgttt caccgacgta
    12421 agcaggtcga tacaatcggg cacttgatac tacgccagcg actggtcttg aagtaaaatg
    12481 attatgttgc atcacattcc acgctgccaa ttgccccaat gtttcaccaa tcaaagaagg
    12541 cataaaacaa agccttccat gatcatcagt ggttaaatag gcatcatctc ttgtgacatg
    12601 ctttatccct ttaatccatt ctcctgggga taattgtaca atgcgatcaa caaataagaa
    12661 cctcatcagg ctacagtttc cgttgcttgc tgctgggcta tagctgaaat aaccagcttg
    12721 caaaaagttt ctacggtaaa caacacagga atttcgttaa ctttcatatt ctctttaaat
    12781 cgatcagcag ggacttcgct caaataattc tttaaagcct gtaatcctga ggttgttaaa
    12841 ataccacctt tctcaaactc atcttcagcc agatcaccac gagcattctt ttccaactgg
    12901 cctctcggga ttttaatctt gaattctttt tccaattgaa aaaccaaatc aagaaaatca
    12961 atggactcag cacccaaatc ctcaattaaa ctactgttta aagaaatttc ttcttcatca
    13021 attactaata catcagcaat aatttcccta accttagggt aaacttcagc tacattcata
    13081 ttttatcctc tgaaccaaca aagctcgcaa agcttgaaat tatatcataa aatttacttt
    13141 tatcatctac tacccaacct gactatagcc agcgtcaaca tataatgtat gggcattaat
    13201 catggccgct tcaggcaaac aaagcaaata gatagcatta gccacatctt caggtgttaa
    13261 cgccctatct gccaaagcat gagcttgata ttcatccagt aactgctctc tgtttggaaa
    13321 atgctttaaa gcatccgtat cgacaacacc tgcagaaaca gtattcacag taataccata
    13381 actagccagc tccaaactta gcgaacgaac caatgcttcc agagctgctt tagaggcgcc
    13441 aataaaagca taatttggaa tcgcccttga tgcacctaag ctggatagtg caataactcg
    13501 actgttttta ggcattaagt caagaccttc cgtaaccaag tggttcaagg ccaaagcatt
    13561 agtttccaaa caccagcgcc aatgcttggt cgacattttc aaagcaggtt ttaatactcc
    13621 actcgctgca ttactcacta gaaaatttaa gtgcttaaag tgttctctaa attgagagaa
    13681 catttctttg acagattgac aatctgctac atcggcttgt aaggcaactg cttttctacc
    13741 catgccggtt atttcattga ccagactttg agcctcatca gagctattat aatacacgat
    13801 agcaatatcg cagcctgact gtgctaactt taatgcggtc gcttttccaa ttcctcgtgc
    13861 accacccgtt atcaatccta cctttcctga aaaaactaat gaactcatac gataacccta
    13921 tagtataaaa tcccaccaaa ctaccgctaa tctgctgtgc tgtcaatgga aatatcggga
    13981 ctctatcaag cggaatttca taatttatct tgtttccaaa gcacttaatt tgtaccatct
    14041 ccacatgata taatttagac atataatgaa tgaagtattc ttggaacaaa actgtttttc
    14101 ctatagcagc aatattctct tttcgtctcc tgggattatt tttacttatc cctgtattta
    14161 ctctttatgc cactaacctt aaaggtgcta ctccaacatt aattggcttg gctttgggaa
    14221 gctatggatt ggcacaagga ttactacaaa tcccttttgg tatgctttca gataaactag
    14281 gcagaaagcc tatgatcgcc ataggtctat tgctattcgc tggaggaagt ttgttaggag
    14341 cagtaactca ttctattgct ggaatgataa ctgccagaat actacagggt gctggagcta
    14401 taggtagcgt gttaattgct ttgcttgctg atttaaccct ggatgaacaa cgtacaaagg
    14461 caatggccat agtagggata actatcggaa catcatttag cctcgcaatg ataattagcc
    14521 ctgctcttac ccactactat ggattatcag gagtgttcta tctcactgca gctttagctg
    14581 ttctagggat tatgatactt tatctcatca ttcccaatcc ctcaaaagag cagtttcatg
    14641 cagatagtga acccagcttg tctctaataa aacctgtggt aacagatcaa caattacaac
    14701 gcctcaattt tggaattttt tgtcagcatt ttattcttac agccactttt tttgctatcc
    14761 ccatgctgtt gaaatatcaa attgagaatg gtcatttgca tcagcagtgg cacttctatt
    14821 tgccactcat gttattctca tttattttaa tggtaccctt tatcattatt tcagaaagaa
    14881 aaaaacgatt aaaaacagta tttgttatat ctgtactgct gacaaccttt acccaaggtc
    14941 tgttagtgct cacatcacaa aattggtatt ggttttgtat tttaatgttt gtgtatttcg
    15001 tttccttcaa tgttcttgaa gcaacattac cttcattagt gtcaaagctg gcaagtccag
    15061 ggagcaaagg cacagcaatg ggtatttatt caacgagtca atttttagga attttttttg
    15121 gtgggtcttt agctggtatt ctttatcagt ggtacgataa ccaagctatt tttctaatta
    15181 acatgattct gagctgtcta tggctcatca tttcgctgag catgaaatct aatgtttatt
    15241 tttctactct tattttgcct tttacagcaa ataatgaaga atcaaaattg gtagtcaatc
    15301 agcttttaaa ggtagccggc attaaagaag ttattttgtc caaagatgaa aacaccttgt
    15361 atgttcgagt agataatgag gaatactcca caggaagcgc tgaaaaaatt cttcatcaat
    15421 ctactattta ccaataacac cttctcgttg ggctgcttcg aaataactgc caaattatgt
    15481 ataataccta actaactttc gcatacagga gatgatttca atggcaagag gaataaataa
    15541 agtaatactt gttggtaatg taggggcaga tcctgatgta aggtatttgc ctaacggaaa
    15601 tgctgttact acactttcag ttgctaccag tgaaacatgg aaagataaaa cgacaggtga
    15661 aaaacaagat agaactgaat ggcacagagt tgtctgtttt aatcgtctgg gtgaaatagc
    15721 cggtgaatac attcgcaaag gttccaagct atatgtcgaa gggagcttaa gaacaagaaa
    15781 atggcaagat caacagggcc aagatagata tacaactgaa attgttgcgt ctgatataca
    15841 aatgctcgat agcaaaggaa gtagtgctac caattacgac gacatgcctt catttcaggg
    15901 atcctctacc cctcaacaag cttcaacaaa gaatcaagca acaccaacat ctactgcaca
    15961 agatgctttt gatgagttag atgatgacat accattttaa atgattcaaa taaaagcccg
    16021 cgttaatgcg ggctcaaaat atagtttata gttattaatc taaaatttac tcttctgtat
    16081 catcactgct tacaggtcta tccattaatt caactatagc cataggtgca ttatcaccat
    16141 ccctataccc acatttaata atacgtatgt acccacctgg tcgttttgcg aaacgtgggc
    16201 ccaaatcagt aaataattta cctacagctg atttagatcg caaaatatta aaagctcgtc
    16261 tacgtgatgc tactgaatca gatttactaa cagtgatcaa aggttcgata tatcgacgta
    16321 aatcttttgc ctttggcaaa gtggttctaa ttaactcgtg ctcaatcaaa gaacaacaca
    16381 tattagaaaa catagcctta cggtggctac tggtacggct aaaactacgt ccggaattac
    16441 ggtgacgcat aacaaagact cctaaatatt actcgccaag gcttgctggc ggccaattct
    16501 caagtttcat acccagcgat aatgatcgag acgctaaaac gtctttaatt tcagttagtg
    16561 atttcttacc aagattaggt gtcttcaata attcactttc tgttctttga accaaatcgc
    16621 ctatataatg aatgttttca gcttttaaac aattagctga tcgaacagtc aactccaaat
    16681 catccactga acgtaataat acaggatcaa aatcattacg ttctttattg tcagcacgtg
    16741 attcttcaaa tttcatatca acaaacgcat gaagctgtct ttgtagtata ctagcagata
    16801 ttcttattgc ctcttcagca tcaatagtac cgttagtttc taattcaata gtaagcttat
    16861 ctaaatctgt tctgttttct acacgtgcac tgtcgacgaa ataagctact ttcttcactg
    16921 gagaaaaact gttgtcaatt tttaattttc caaccgtctt tttctcaatt tcatcatcat
    16981 gatagcgaac aaatgcatca gtattatgaa agccaatacc tctttcaact ttaagagtca
    17041 tatttaattt acccttttca ttgagattgg ctatgacaag ttctggatta ataatttctt
    17101 gaccatgagt caactgaata tcaccagctg taacttgaca tggtccttct ttatttaggg
    17161 taacctgcgc ttcgtttcct acagtcaatt tgatagcgac tgattttagg ttaagcaaca
    17221 aatcaaccac atcttcctga acaccttcaa tagtactata ttcatgcaaa acaccatcga
    17281 tagaagcttc agtaatggca ctgcctggca tggatgacaa taagattctt ctcaaagcat
    17341 tgccgagggt gtgaccaaaa cctcgctcca aaggttctaa aactattcta gctttatagg
    17401 gcgactcggc ctgtactttg agaaccttag gcgtcaacat ttcattgatt tcagtataca
    17461 ttcagctatc tctccgagct tacttagagt aaagttctac aacaaggttt acgttgtagt
    17521 ctgaagataa atccattaac gttggtgctg tagaaaatgt acctttaaaa gaaccagtat
    17581 ctacagtaat ccaatcacag ggtgctcttt gttcagataa agctaaagca gcttggatac
    17641 gtccttggtt tttagccttt tgtcgaactg atatagtatc cccaggatta accaagaaag
    17701 aaggtacgtt aactacttta tcatttacaa gtattgcctt atgtgcaact agttgtctag
    17761 cttcagcacg tgtactggca aaacccatac ggtaaacaac attatctaaa cgtctttcca
    17821 atagggccat taaattttca cctgtagaac ccttttgtct agccgctttt ttgtaataat
    17881 tacggaattg cttttcaaga atcccataaa gccttctaat cttttgtttc tctctaagct
    17941 gaattccata actatttaaa cgaggttttt tatcaccatg ctgtccaggt aatttttcag
    18001 atttacactt ggatttatga tcacgaaccc cacttttaag taacaaatca caaccttcac
    18061 gtcgtgataa tttacattta ggaccaagat atctagccat taattactcc tgagtcttat
    18121 acacgacgtt ttttaggtgg cttacaacca ttatgtggta tgccagttac atcggtaatt
    18181 tcaacaattt taaaatcctg ggttattaat tcacgaatag tagattcacg accaggacca
    18241 ggaccatgca caaaaactgc aacagatttc ataccatatt ctttagcaac agcagcagct
    18301 cgctcagttg caacttgcgc agcataagga gtactttttc tagaacctct gaagccagat
    18361 ccaccagaag ttgcccagca taatgcatta ccttgtctat ctgtaaaggt aactatagta
    18421 ttattgaaag atgcatgaat gtgtactatg ccatcagaca ctacgcgttt tacctttttt
    18481 cgtgtttttt gctgctttga ctttgttatt gccatctttt attttcctac atgaaaaatc
    18541 aactactagt gccttttcta cggccttttc tggtacgagc attagttttc gtacgttgtc
    18601 cgcgcaacgg caatcctctt ctgtgtctta atcctctata acaaccaaga tccattagtc
    18661 tttttatgtt cattgttaca acacgtcgaa gatcaccctc tacagtaatt ttcgcaattt
    18721 cagttctcaa agattccaac tgagcatctg ataattgaga aactttaact gaaggatcta
    18781 tatcaactgt tttacataat tttaatgatg ttgtcttacc aataccataa attgctgtta
    18841 aagcgattac cacatgttta tgatcaggta tattaactcc tgcaatacga gccattagtt
    18901 tctccgaacg ttatttgttc tgtcgaaagg gacagaagga tactatttta ataaaataaa
    18961 atcaagcaga tattaccctt gcttttgttt atgacgggca tccttacaaa ttactcgtat
    19021 agtgccactt ctttttatta ttttacaatt gcgacatatt cgctttactg atgctcttac
    19081 tttcattcct aactccgata aatcatagca gaccaggtaa cttagtacct ctaaaattcg
    19141 ccttttttaa taaagaatca tattgctgag tcattaaatg agcttgtacc tgtgctacaa
    19201 aatccataat aacaacaact ataatcagca acgaagtacc accaaaataa aatggaacat
    19261 gccaggtata cattagaatt tgtggtaaca aacatactag aaccaagtaa atggctccaa
    19321 ctaaagtcaa acgagtcatc actgaatcga tatacttggt tgtttgttct cctggacgaa
    19381 ttccaggaat atatgctcct gattttttca ggttatctgc tgtatcctta gggttaaaca
    19441 ctaaggcagc ataaaagaat gcaaagaaca aaatagctgc tgcatacact atcaaatata
    19501 agggttgacc tggtgatagt gccattccaa catcagataa ccatcccagc cctttagttt
    19561 gtgaaaaaaa ttgagctaaa gttgctggca ataaaataat gctagatgca aatattggtg
    19621 gaatcacacc tgacatattt atttttaatg gtaaatgact cgtttgggct gcatatacct
    19681 ttctaccttg cgtcctctgt gcataattaa cacgaattcg tcgttgagca cgctccataa
    19741 atacaacaaa accagttact acaattacta ctgctgcaat aataactagt gtgagggctt
    19801 gcatctgacc ttccttaact tgctggagga cagatcctat agcatttggc atgctagaga
    19861 caattccaga aaagataatt aaggaaattc cattaccaac acctttctcg gtaatttgct
    19921 cacccaacca cattaaaaac atagtgcctg taactaaagt tacaacggca gtaaagtaga
    19981 atgagaaatc tggttgcata gcaatttgct gactagccaa ccatctcgcc atacccatgg
    20041 actgaaatat tgacaagagc aatgttaaat atctggtata ttgatttatt tttctacgcc
    20101 ctgattctcc ctctttcttt agttgttcca gcttgggaga aactacagaa aacagttgaa
    20161 taataattga tgcagaaata taaggcataa taccaatcgc aaacactgtt acacgagaca
    20221 gtgctccacc agaaaacata ttaaacaagc caaatattgt gttttgctgt tcactaaaaa
    20281 aattagctaa ttttgcagga tctaatcctg gaacaggtat atgagcacct aatcggtaaa
    20341 ctaatatgcc gagaacaaca aacaacagtc ttgactttag ttcggctaat ccaccacgag
    20401 gcttactacc gttatgcttt tggtttttca tagtcactct tctatactac cgcctaaact
    20461 ctcaatagca gaacgagctc ctttcgtgac tcttaaaccc ttaagcttaa ttgctgcagt
    20521 cagctcgcca gataaaatta ccttaacatc tttgattgag ttattaatta aacctgcttt
    20581 acgtaaagca actaaatcaa ttacttcatc attcaatttt gcaagctcac ccaaactcac
    20641 ttcatcaata gttcgaccta ctctagactt gaaacccatc ttgggtaacc gtctctgtat
    20701 aggcatttga ccaccttcaa aattaatttt gtggtaacca cctgctcttg atttttgacc
    20761 tttatgacct ttgccacaag ttttgcctaa tccagaacca atacctctcc ccactcgtct
    20821 tcttgagggt ctggaacctg gatctggcga taatgtattt aaattcatta tgcactctcc
    20881 tcaaccaaca acaggtaatt aatttcattt acaagtccac gaattgcagg agtatcacta
    20941 tgaaccacac ttgaatttgt tttacctaaa cccagctgtt tggctatagc aacatgtttt
    21001 ggttttctac ctatcgtgct tttaaccaag gttattttaa ttttttttcc cataattagc
    21061 ctgccattac ttcttcaact gtttttccac gtttggctgc tacatagtcc ggcgtaccaa
    21121 tgtgtgtcaa tgcaccaata gtggcacgaa caatgttact tggattggta gaaccaatgc
    21181 tttttgcaag aatattttgc acacccaaca cttccaaaac ggcacgcata gcgcccccag
    21241 caataattcc tgtaccttca cttgctggct tcataaacac cttggaagct ccgtagttcc
    21301 atgtgatttc gtggaatatt gtagtgcctg aaagaggaat gtaaaccata tttttcttgg
    21361 cttggtccat agctttttga attgctattg gaacctctct tgccttacct cgtccaaaac
    21421 ctactttacc ctttccgtca cctacaacta ctaaaacagc aaaaccaaaa actcgcccac
    21481 cctttacaac tttagccgta cgagttaccg ataccaactt ttcttggtat ccatctgact
    21541 tgggtaattc atcgaatgac ataatcgttc cttaaaaatc taatccggct tcgcgtgcac
    21601 cttcagcaag tgctttaact ctgccatgat atttataacc tgctctgtca aatgcaacct
    21661 gtgtaatgcc tgcagcctta gcacgcttac ccaacagttg accaactaaa ctggcttgtt
    21721 caactttact ttttccagtc aaactggatc tcaattcttt atctatagta gatgatgcta
    21781 ccaacacctt atcaccaagc tggtctgact tgacaatttg agaataaata tgcacaccac
    21841 ttcgatacac aactaatctt gagcggcctg ttttgcgtat taaggctttg gcttttaatc
    21901 cacgtctatg tcgtgcgttt tgcttattca tagttaacac cttatttctt cttagcttct
    21961 ttacgaacaa tctgctctcc agcatatttc acacctttcc ctttataagg ctctggtggg
    22021 cgaaatgctc taatttcaga agctatttgt ccaagaactt gcttatcaac gcccttcagc
    22081 aaaatggttg tgttattagg agtttctacg gttacacctt ttggtaaatc atactctata
    22141 gaatgtgagt atcctaatga caaactaatt gacttgtctt tagattgcgc tctatatcct
    22201 acaccaacca attctagtgt aactgtatat ccctcggtaa ctccttgaac catgtttcta
    22261 actaaggctc tagccgtacc agcttgtgcc catcccattg ggtcattaga agcaggtgaa
    22321 aattctaatt tgtttgattc cttactatgt tgaatattaa ccaatttatt aattttttga
    22381 gttaatattc ctttggggcc ttttacagtt attactccat caacaaatgt tacttcgaca
    22441 tttgctgaat gaattactgg ggcctttgct actctagaca tatcattcct cgcaagtatt
    22501 aagccacttc acaaatgact tcgccaccaa caccttttac ttttgctgat acatgggtca
    22561 ttataccttt agacgtagaa agaatagcca caccaaatcc tgggattgaa tgcaaatcct
    22621 tatatgattt atatactctc aaacctggac gactaatgcg cttaataaat tcaattaccg
    22681 gtcttccatg atagtacttt agttttaaag taatcgactt cagaccattt ggtaatgttt
    22741 cagtaaaaaa gtcttgtata tatccttctt cctttaaaac tcgagctatt tcttccttta
    22801 atttagaaga aaccaatgtt acttgctgat gtttagcttg ttgaccattt ctaattctag
    22861 tcagcatatc agcaattgga tcatgcatac tcacaattat tctccaataa aatagtttac
    22921 cagcttgatt ttctaccgcc agggatattg cccaccataa gctgttgtct taaacaaatt
    22981 ctgcaaagat taaacttgcg ataaactgcg tgtggtcttc cacattgctt gcatcgagta
    23041 acatagcgaa caggatttga attaactggt agttttgcta gttttgcctg actttccatt
    23101 attacctgga aatcatcaga tgatttaatc aattgtttta actcgtttcg gcgttgtctg
    23161 tacttttcta ccagcttcgc acgctttgac tctcgcatca gcattgattt tttagccaca
    23221 atttcaccct tttattttct atctttgtct ttcagtggaa gattaaatgc ttctaacaaa
    23281 gctttagctt cttcatttgt tttagcactt gttgtaatac aaatatctaa accgcgtatt
    23341 ccgtctgttt tatcataatc aatctctgga aacacgattt gctcatgtat tcccatgctg
    23401 tagtttcctg ttccatcaaa agattttgga ttcaaaccac gaaaatctct tacacgaggt
    23461 aaagtaatag atattaatct atctagaaac tcatacatac gttcacgtct gagcgttacc
    23521 ttacaaccta ttggccatcc ctctcttatt ttaaaaccag ctattgattt tctggctttt
    23581 gtaacgactg gtttctgacc agaaatcagc gtcatgtctt caatcgcatg attcatgact
    23641 tttttgtcac caacagcttc tccaactccc atattaagag ttattttgag aatcctgggt
    23701 acttccatca cactcgaata gttgaaccgt ttcatcatca tagtgacgac gtctttctta
    23761 taaaattctt taagtctcgc catttcaatc acctaactct atatacggtc aattatttca
    23821 ttgttagact tataatatct aacctttttg ctaacaccat tactctcaag atatttaaag
    23881 ccgaccttat cagccttttt tgtaacagga ttatagtgag caacatttga aacgtgcaat
    23941 ggagcttcac gagctataat accaccctta ttttcaggct tttgtggatt aggcttgata
    24001 tgctttttaa ttagatttcc gccttctact acaacggcgt catcaactac ccttaaaacc
    24061 ttacctatat gacctttact tttaccggca attacaatta cttcatcgcc tgatttaata
    24121 cgtttcatat ctaatccttc ttacaaaacc tctgcggcaa gagaaattat cttcataaat
    24181 ctttctctta actcgcgtgt aaccgggcca aatatacgtg ttcctattgg ctcgttttgg
    24241 ttgtttaaaa gaactgctgc attatcatca aatcgaatca atgaaccatc atctcttcga
    24301 acaccttgag cagttctaac gactaccgct ctcattacag cacccttttt taccttactt
    24361 cttggtatag catcttttac gcttacttta attacatcac ctacacgggc ataccgtctg
    24421 tgtgatccac caagtacttt aatacacatt actttacgtg ctccgctgtt atcagctact
    24481 tcaagcactg tctgcatttg gatcattttt ctctccagat ctaatttcga ccagaaaaag
    24541 gctgctgatt atatcaggcc ttttagggtt ttaaaagtaa aacaattaag ttacttatga
    24601 aattacttcg actaataccc agcttttcgt tttagagagt ggtcgtgact cacggatttt
    24661 tacggtatta ccaactcggc aaacttgatt ttcatcatgt gcgtgtagtt tagttcttcg
    24721 cttcatgatt ttcccatact ttggatgttt cacagtacgt tcaatcataa ctacaatagt
    24781 tttatccatt ttgtcgctaa ctacttttcc aatcattgtt ctggcatttg attcactatt
    24841 agtagacatt tcacttacct gccttttcag tcaagattgt tttaactttt gccactgact
    24901 tacgcaccat agtgatcaga tgtgttttat ctagtgagcc acttgctttt ttcattcgta
    24961 aattaaattg atctttacgt aatgaaagca attcattttg caattcctca actgacatat
    25021 tgcgcaattc atctattttt ttcattacat taccgtccgc tcttcaaaca tcactttgaa
    25081 aggaagtttt gcttttgcaa ggtcaaatgc ttccattgca agctctttgc taacgccttc
    25141 catttcaaat aaaaccttac ctggttgtat ttgggctacc caatactcaa cacttccctt
    25201 acccttacct tgtcttactt caagtggttt ttgtgtgatt ggtttatctg gaaatactct
    25261 gatccaaatt ttaccaccac gcttaatatg tcgtgtcata gcccttcgcg ctgcttcgat
    25321 ttgtctagca gttaaacgtc cacgctctac agcctttaga ccaaactcac cgaaacttat
    25381 tttactacca cgaagagcca aacctctatt gcgacccttc atttgctttc ggtacttagt
    25441 acgctttggt tgtaacataa tttctaatcc tcactcactg ggcgctttca gttgatcttt
    25501 tcttttgcgg gagaatttca cccttaaaga tccagacttt tacaccaata attccgtagg
    25561 tagtcttgga ttcagcggta ccataatcaa tatcagctct aaaagtatgt aaaggcactc
    25621 gtccctctct gtaccattca cttcttgcaa tttcagcacc acccaatcgg ccacttacac
    25681 aaattttaat accctttgct ccagctttca gagcagaggt aacagcacgt ttcatagctc
    25741 gtctgaacat gactcgttgc tctaactgct gagcaatacc ttcagccacc aaagtagcat
    25801 ccaactcagg ctttttcact tcttcaatat tcaaatgaac tggcacaccc aggttatcgg
    25861 aaatttcttt acgaagtgtt tcaattcctc cgcctttttt accgataata acacctggtc
    25921 ttgctgttaa gatagtcaca acagcgttat tagctggtcg ctcaataagt atcttgctaa
    25981 cagctgcagc catcaatttc tttttaagat cgttacgtag ttttatatcc tgaattaaaa
    26041 actcagcata tcgctttcct gcaaaccatt ttgagttcca atctttaatt attccaaggc
    26101 gtatacctat tgggtttact ttttgtccca ttgctattcc tcgtcagaca ctttaatagt
    26161 tatatggcag gtacgtttgc aaattcgatt tgctctaccc ttagctctgg gactaatacg
    26221 ttttaaagtt gttgcttcat caacgcatac catgcctact ttcagatcat caatatcagc
    26281 tccattatta ttttcagcat ttgctatagc tgactctaat aatttaagca ttaatttagc
    26341 accctttttt ggtgtaaact tgaggacatc aagcgcacca gacacattca tattacgtat
    26401 catatcggct actaatctac ccttttgtgc ggataaagga gcgcctttta atttagctgt
    26461 tacttccatc atatcctctt acttaccttt agcctttctg tccccagagt ggcccttgaa
    26521 tgtacgagtc atggcaaact cacctaattt atgaccaacc atattgtctg taataaatac
    26581 aggaacgtgg tctttgccgt tatgcacagc aattgttaaa tcaatcattt caggaacgat
    26641 tgtagaacgt ctagaccaag ttttaattgg cttttttgat tttgattcga ttgcagcttc
    26701 taccttgctg atcaaatgat ggtcaataaa tggacctttt ctaattgaac gagccacttg
    26761 atatcctcat attaattatt tcttcttacg tcctctgaca ataaatgtgt cagtacgctt
    26821 attgcttcta gttttatagc cttttgttgg caatccccat ggagatacag gatgacgtcc
    26881 acctgatgtt cgtccttcac caccaccatg tggatgatca actgggttca tggcaactcc
    26941 acgaacagtg ggacgtatcc ctctccatcg tttggcacca gctttaccca atgctcttag
    27001 gttgtgttcg ctattactta cttcaccaat tacagctcgg cataaaacat gtatcttgcg
    27061 catctctcct gaacgtaatc ttaaagttgc ataaacacct tcttttgcaa caagttgtcc
    27121 actacaacca gcacttctga gcatttgcgc acctttacca ggcttcattt caacacaatg
    27181 tattgttgta cctacaggta tattttttag tggcagacaa ttccctacac taattggtga
    27241 atcagctcca ctttggatag ttgctccaac ttccagattt gatggtgcta taatatagcg
    27301 cttttcacca tctttgtagg atatcagtgc aattagcgca gttctatttg gatcatattc
    27361 caatctctca acacgaccta atataccatc cttattgcgc ttaaagtcta taattcgata
    27421 tttttgacgt tgtccaccac caatatgtct aacagttata cgaccttggt tgtttcttcc
    27481 acctgttttt tttagttttt caactaatgc agcatgtggc tttcctttat agatatcgtg
    27541 atgtacaaca cgtatttcac ctcgttttcc gggtgaagtc ggtttagatt ttaatagtgc
    27601 catctttatc tgccttattc agtaactgta aagtcaatat cttggtctgc atgaagagag
    27661 acaaatgcct tcttccagtc acttcgcttc ccactggttt gtttgaagcg tttagattta
    27721 cccttcacat tgacaacaga tacactttta acttttacat taaatatatg ttcaactgct
    27781 aatttaattt cagtcttagt cgcatttttc agtactttga aagtgaactg tttaaactta
    27841 tcggccatga cagttgcctt ttcagaagta tgtggttctc taagaaccat catcaatctc
    27901 tcagcgttca ttgtaactgt tcctcaattt ttttaattgc agcttctgta acaacaactt
    27961 tttcaaatcg tagtaaggaa acaggatcta tagttgtaac atcacagata tcataatcaa
    28021 tcaggtttct tgaaccaagg tattcatttt caccaacttc actcattata atcagagcat
    28081 tacttatttc catctgattc atttttttga ggaaatcctt ggtcttgtga ttatcacatt
    28141 ggaaatcact aacaaccacc aggttgcctg tgcggcaaag ttcggagatt atactacgta
    28201 atgcacgttt gtacattttt ttattaagct tttgtgaata atctcttttt ttagatgcaa
    28261 atgttactcc accacttctc cataatgggc tgcgaatggt tcctgctcgc gcccgacctg
    28321 ttcctttttg gttccagggt tttttaccac cgccactaac ttcagaacga gttttctgag
    28381 cgctattacc gcttcgagca ttattcataa aagtaactac tgcttgatga accaagcctt
    28441 cgttgtatgt ataagcaaat atctctttat ttagctttaa ttttgacttt gtatctattg
    28501 tagtaatttc catcactcac ctcgtgcttg ctttttgact gcaggcttaa tttctactct
    28561 agaacctgga gcaccaggaa ttgcaccttt aatgagcaat aaatttcttt cagagtcaac
    28621 ttttaccaat tccagacttt gaattgtaca tcgagcatta cccatgtgac cggccatttt
    28681 tttcccttta aaaacgcgac caggggtttg gttttgtcca atagaaccag gtactctatg
    28741 tgatcttgag ttaccatgcg atgcatcttg agttctaaaa ttatgtcgtt ttacagttcc
    28801 tgcgaaacct ttacctttag tcagacccga tacatcaaca tattgtccgg cagtgaatac
    28861 atcagcaaca gatatgactt gacccggtgt aaatgcatcc tctgaatcta tacgaaactc
    28921 tacttgcata tcaccagcat caatttgtgc tttagcaaaa tgcccagcca ctggcttact
    28981 cactttgcta gatttctttg taccaccagt taattgtaca gctgaataac catcattcgc
    29041 tgctgttttt acctgagaaa cgcgattcgg ctgaacctca acaacagaaa caggaacaga
    29101 aactccttct ggtgtgaaga cacgcgtcat accgatctta cggcctaata aaccgatcat
    29161 cattgtaaca cctctttaat atcctttgcc tttatattta gtcatcaagg cttatctgaa
    29221 catcaacccc ggcagccaaa tccaatttca ttaaagcatc tacagttttt tcagttggat
    29281 gaacaataac gaccaggcgt ttatgagtac gtaattcata ttgatctctt gcatctttat
    29341 tcacatgcgg tgaggtcaat acagtaaatt tttcttttct gataggcaaa ggaattggtc
    29401 cacgtatctg tgcgccagtt cttttcgcag tatcaacgat ttctcgtgtt gatagatcaa
    29461 tcaaccgatg atcaaaggat tttaatctta ttttaatatt ttgattgctg ctcattt
//
LOCUS       DAXLDT010000023        29404 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXLDT010000023 DAXLDT010000000
VERSION     DAXLDT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29404
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.023"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(2..763)
                     /locus_tag="ACF2SP_000838"
     CDS             complement(2..763)
                     /locus_tag="ACF2SP_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358640.1"
                     /translation="MKLHSISTNKMRLIISMWLIYLFAVLFLVASLFTMLILMMEYTL
                     VCKNKQYHLAKECVLHSKIYNLYHSSTELGDLKAAVVKSSRSSKGNTLYYVDLLTAQG
                     PVNLTGSSSSGRDDKEIAANSINNYISNSLETTFKVSYPASWWMYGLSVFFLLVGAVL
                     LTVRGAITDFDRILKTIVIKRKGLFKTEETKLSFSDVDKIIVEESLGSRGTKTYRLAI
                     ALKDKPPIPLVTTYDSSFAKKEKIANQLNQFIHEN"
     gene            1027..2052
                     /locus_tag="ACF2SP_000839"
     CDS             1027..2052
                     /locus_tag="ACF2SP_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358641.1"
                     /translation="MGWTDRFWNAYRVVTNTVIEGAANSLNAAGSLACLLGGAGVSLS
                     YSMSETIKAGYFGSVGVAGTVNLDVTAVEFNYGFNQTIPFRRDLLEKSGGGSYALNDF
                     ANPGTVFMISSLAILSGTTLRTFGANLKKWQQNREDKYHFIAKYSTDLEPPSYKEYLY
                     VNAESFFNSLAIASFSSLLAKNVLDFSIKDLSKYHFTYPFNGTNITGSVKTPDYTGPV
                     TSEFFPLDFFVSQNTTIHTDYFNLIVNEKVKANAMTNVTYGGGVHLEPSHQSSAPLLA
                     ASATIGIGTYLAGTFFGKKILRERDERLQRSRSSSYSIVSGTFWDNKHDIEAKGDTKF
                     EHTHSLQ"
     gene            2114..3307
                     /gene="lapB"
                     /locus_tag="ACF2SP_000840"
     CDS             2114..3307
                     /gene="lapB"
                     /locus_tag="ACF2SP_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase LapB"
                     /protein_id="HFD2358642.1"
                     /translation="MVFKSRLVGVILLCFSSITGWCLSQSHEEDRIIVSNCLYEQLKD
                     KARLIAQSDHFSFIAIPIDENIANTLQKMRPVCGNYLNAEPYIQQTYNRFLDSQKLLD
                     KLTRYHSISTANPYPINHEAQVHGLFEQIDPAKIWQTNQHLTSYINRSAKSRTGVEAA
                     QWFKQQFDTLAQDYGRKDVESYFVKTGNKFIQPSVVTVIGKDKPGEAIVIGAHIDTLD
                     GNMPGADDDSSGISVELEMARVLFSSNFELNRPVYFIAYAAEERGLIGSGYVVQDFLQ
                     KKILVKAVMQLDQAGYRANAKDQTIWLLKDYVDKGLTEFTAELLTRYVKIPVGYTKCG
                     YACSDHVNWTNEGFKTTYPSATTLDDDNPYVHTSNDTLDILNLEHMVNFTKLGLAFIV
                     ELGLN"
     gene            3366..4256
                     /locus_tag="ACF2SP_000841"
     CDS             3366..4256
                     /locus_tag="ACF2SP_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358643.1"
                     /translation="MKTEALLSWLNVESNESYDSIRIKYQLRLEALEKEERIKKMGGI
                     NPFAPKIPLNNTRIITSKEIQEAWAAIDSLSKYQKFKGLVAQEENLSTIPNKKMDSKR
                     EETKLIHFVRERAQGPQGRRLPAHLQAKIKRASLDKPHSKLITEGVEQQINAILDKTT
                     LSASDLQSAVYTAVQNYTNYHMKSENKNNPKYNRGQGDGFFSFFRHGSFGITTANNLY
                     TSISAKNATLDETIEKLKDFLGHSSRAYHHHSFTSYLADALAEKGLLTSHPASRYKQE
                     DVVNQLDQWIAQNSTVNLLK"
     gene            complement(4368..7298)
                     /locus_tag="ACF2SP_000842"
     CDS             complement(4368..7298)
                     /locus_tag="ACF2SP_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2358644.1"
                     /translation="MIDKMRKSKKLHPLNHIPSGDNFSHLNLLERSLISQLIELSYDE
                     NTLHTLSLSGLKLLGKHPLLKDTITAVWHHDGSHINILASNGLKFDAPKELEKLLAVV
                     SDWHFLKIYKTKLKKHSEEKLKQRVDICLDKYQGNLFCISFWFDSIDCNFEILMPFFK
                     DILGILNLSLARLMNKKREKLLTTILEKAGDSVEITNEEAVIQYVNPAFENITLYSAR
                     EAMNKTVASLLRSPVEDVQLFEEINQTLKSGNTWKGQIRSRKKDGSYWVAQTIIVPVM
                     DDELGRITQHIAIKQDITEQVHRFNQLKISEERYRNLMNAVSDGIFIHNLQGAFLEVN
                     EAACLSLNYSREELINSYVWDIEVGFSQESLNQLWENVQKGPINTEGMHRRKDGTTFP
                     VDVRLAVFNATGEKLVLAIVRDITLQKRSEATIRKLTRALEQSPVLVIITDKTGTIEY
                     VNAKVIEQTGYRKEELLGGNTRILRSGQTPHEIYKTMWEHLANGEEWHGELLNKNKSG
                     EHFWVSAIISPIRNENEEITHYLAVMEDVTQKKSYEEMLKYQATFDNLTNLPNRLYAV
                     NKLERAISKSHINNQKLAVLFLDLDEFKQINDSLGHAVGDLLLKALSERFLSMIRQTD
                     TIARLGGDEFMMILENIMQDKDAESIAKKCREICSQPFVIESQELSVSTSIGIAIYPD
                     HGKDAKTLMRNADTAMYQSKIRGKNNWTVFQNTMIEGANNHIRIKTELHQAFTRNELY
                     ICYQPIIDLKKNKVVAAEALLRWQNTTLGQINPDQIIPLAEETGMIIPLGYWILDKVC
                     AQAKEWEFITGSKIKLAVNISTLQLKQKDFIHKVQLILKRTKLSADSLIFEVTESAFI
                     DDSKFILSQLNKLSDMGIHFSLDDFGVGYSFLQYIKSYPFKNLKIDRVFIQGVNTNHN
                     ELSLINSIITMARNLKLSVIAEGIETAEQLELMRSMNCDMVQGWYFSKPLSGDKFLFF
                     LMNK"
     gene            7521..8162
                     /gene="coq7"
                     /locus_tag="ACF2SP_000843"
     CDS             7521..8162
                     /gene="coq7"
                     /locus_tag="ACF2SP_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HFD2358645.1"
                     /translation="MRTSSFLDRLISEVDSALRTLVLPPKRITTRQSPAENLADTVLS
                     AQEKKHISGLMRVNHAGEVCAQALYQGQALTAQLTHIKEQMASAAAEEVDHLAWCEER
                     LYELGSKPSLLNPIWYCGSVLLGALAGLAGDKVSLGFVAETERQVTAHLQRHLHYLPE
                     KDKKTIAILKRMQEDEEHHAHTAMEAGAVELPYIIKQLMNAVSKLMTQSSYYI"
     gene            8179..9174
                     /locus_tag="ACF2SP_000844"
     CDS             8179..9174
                     /locus_tag="ACF2SP_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFD2358646.1"
                     /translation="MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQ
                     AVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGL
                     PSSSSHALIGGLAGAAIAKGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
                     LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPF
                     WVVISCHGVISLGTLAGGWRIVHTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPV
                     STTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALVAAAIMTISPTA"
     gene            complement(9184..10575)
                     /locus_tag="ACF2SP_000845"
     CDS             complement(9184..10575)
                     /locus_tag="ACF2SP_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD2358647.1"
                     /translation="MDLFRKKDIKGSLDNESHLAKCLSVFDLTFLGVGAIIGAGIFVL
                     TGIVAATISGPAVIFSYVVAGFACAFAALSYAELAASIGGCGSAYGYAYAGFGELIAW
                     IVGWDLLLEYSIAVSAVSIGWSGYANDFLMALKIFIPTHLLHGPADGGDLNILAILII
                     AVLTTLLIVGVKSSSRFNNIMVFVKLLVIFIFIAIAFGEVKVENWSSFMPYGWAGVMK
                     GASIIFFAYVGFDAVSTAAEEAINPQRDLPIGIIASLAICTVIYILVSGLLTGIAHYT
                     TLNVSSPISHVLLVLGYKTAAGLVGVGAIAGLTTVMLVLFYGLTRVFLAMSRDGLLPR
                     FLSKTNEHTKTPIRIILLCGILMASLASVTPIGTLAELVNIGTLFAFIVVCGGVLYLR
                     RTHPDMERPFKTPFMPYVPILGIISCFYLILNLPWVTILRFIIWMLVGIAVYFAFSRT
                     NSALSVEGDCAAE"
     gene            10735..11307
                     /gene="pagP"
                     /locus_tag="ACF2SP_000846"
     CDS             10735..11307
                     /gene="pagP"
                     /locus_tag="ACF2SP_000846"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFD2358648.1"
                     /translation="MKRLISCLTIICALNASAAAETTSNPCSRWISFLKPVCQRFHQT
                     WTEGHDDMYFSGYAWHNRYVYSNEKIKSYNETAWGGGLGKSLFDEKGNWHGLYAIAFL
                     DSHRHLEPAVGYAYLKTASVNKDLKAGLGYSVLVTSRVDYDNVPIPGALPWAALFYKR
                     ITIAATYIPGSSREGHENGNVLYMLGKISL"
     gene            11447..11872
                     /locus_tag="ACF2SP_000847"
     CDS             11447..11872
                     /locus_tag="ACF2SP_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HFD2358649.1"
                     /translation="MMLKTQLTAFIGAVILAGSSLANPIKPEVCPSVPSIQSEGMSMS
                     SEILEGMYITYNLSHYNTSSSWVFIVGPIAAENDDMALAESNKLLSTMSGSPHPEDDG
                     EGNWICQYTTKSKDIIAFAIEADDMLSPLKMMRYLRTIR"
     gene            12019..12567
                     /locus_tag="ACF2SP_000848"
     CDS             12019..12567
                     /locus_tag="ACF2SP_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HFD2358650.1"
                     /translation="MKSFIEQAQFYATYHQNQATRYTHMAGVPLIMLSIMIFLGFVKI
                     VIPGVYATDLACLATLAALIYYFLLNWQLALALTPILIFLLWLASWFNYDGPTKFGLW
                     VFIITFVVGWGLQFYGHFIEGKKPAFMVNFTQALIAPLYLTAELFFMAGLMNSLREQI
                     YGNEEVNTEEISKSKTKTKSKS"
     gene            complement(12637..14760)
                     /gene="rlmKL"
                     /locus_tag="ACF2SP_000849"
     CDS             complement(12637..14760)
                     /gene="rlmKL"
                     /locus_tag="ACF2SP_000849"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFD2358651.1"
                     /translation="MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPV
                     IYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQ
                     IRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
                     HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAH
                     IAPGLLRQDQSLQYWARHQSSLWEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNA
                     ERAGVLPLVEFNTLPLNACRPGTKRGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTC
                     YQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPDAPLS
                     DNAQMLFNRLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPP
                     ASIPVHKAEKRSLEMLQVVPRALGIHPEKLIVKQRKQQKGSEQYQKIGKTSQRLIVTE
                     GKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAALAGALT
                     TNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNS
                     KRMSDILDIQRDHVSLINMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITPQT
                     IDQDFKRNSKIHHCFKIVMPHFADN"
     gene            complement(14757..15236)
                     /gene="yjgA"
                     /locus_tag="ACF2SP_000850"
     CDS             complement(14757..15236)
                     /gene="yjgA"
                     /locus_tag="ACF2SP_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFD2358652.1"
                     /translation="MDEQISKSQKKRDAHYLQDIGIKFIDLSASKLDSLPLPDNLRQA
                     IIDARSIKSHGAKRRQAQLIGKLMRAADFEAILAAYEKILEEESSITACFHEIELWRD
                     RLLQDGKDALTEFIDAYQPEDVQQLRQLIKKALDDQMKEKNTGAAKALFRYLRSLIK"
     gene            complement(15427..16338)
                     /locus_tag="ACF2SP_000851"
     CDS             complement(15427..16338)
                     /locus_tag="ACF2SP_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFD2358653.1"
                     /translation="MAFFLITLCIIILSFFCLAVMVLKFMQQPSLPLSFSQYVKLMCS
                     GVIAFIADTVGVGSFAVNVALAKLLGTFRDDELPAVNNGAQVLPGAIESLFFIHMVDV
                     DLTTLLTLVTGTCIGGLVGGFVVSQLSKQAIRLAMICSFALIIVLLIAHQLRLLPVGG
                     DLTELHSWKLVIGFFAMVVCGSLTSVGVGLFVMVQGVLFLMNVSPVVAFPIMTTAGAM
                     QQPLTTLVFLQKNKIPLKKTLILSFSGCLGVMITIPIFTKLTITWLHFLLLFILIYNF
                     LAIGHTYLRSRPVKQYVQSSVKLAAAD"
     gene            complement(16559..18235)
                     /locus_tag="ACF2SP_000852"
     CDS             complement(16559..18235)
                     /locus_tag="ACF2SP_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HFD2358654.1"
                     /translation="MLKKIMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKN
                     AIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQSN
                     DNAEVNGHLLDNIELNTKPAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNN
                     QLKLVYLVSFKSVQQDGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYW
                     YGKDGLPALDVTQNGSQCVMENSKVKLVNLHSQWDWENTINTPFEYVCNNNIEENING
                     GFSPGNDAYYFGHVIVDMYKDWYGLNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAM
                     SFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLL
                     EKNPQLYNKAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNN
                     GSYCAINYDEVVAYANAHIALPQERQSFIVHTASGVFNKAFYLMSKDMGIKNAYHIMV
                     VANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDIAGCAI"
     gene            complement(18470..19855)
                     /locus_tag="ACF2SP_000853"
     CDS             complement(18470..19855)
                     /locus_tag="ACF2SP_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2358655.1"
                     /translation="MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNID
                     SKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLD
                     LMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
                     QITASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQ
                     GELEVTERNIVISRNALRYLLNQNPGEIKTVKKLLQLKNHKLIPGSLPLTVLENRPDM
                     QVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPI
                     IDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHL
                     AKAYNLNDRLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYR
                     AKLESEIKSSV"
     gene            complement(19839..20744)
                     /locus_tag="ACF2SP_000854"
     CDS             complement(19839..20744)
                     /locus_tag="ACF2SP_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD2358656.1"
                     /translation="MRRWLGVQAFSYVELRSIQISTVFAFTIFVQEWLRYPRAGWTGF
                     AVMMIYAGFDNGTTIFRTYHRFLGVLLGLFTGYLLWFVGHLDYRTLILIMPLMVFFAF
                     FLVGRAYSIPTVFTVNVSVLGSGYFSTEDTLPVTYFIIDYTLCTLIAFTIILGFEYFW
                     FRHYGLMQRFIKSTQAEVIDDLYKLVRLLNQGKIKKTEWFQACIKLTDSLFEVNKLIT
                     NSQFLISSEHAVGDEFNQFVELANRIFIGLKALYMAYYTKHYHTFDYYQLFQQVQKDL
                     VHLKDFVAGEQTIDLSEGVKHATSG"
     gene            complement(20729..21661)
                     /locus_tag="ACF2SP_000855"
     CDS             complement(20729..21661)
                     /locus_tag="ACF2SP_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD2358657.1"
                     /translation="MFISQLKDKLASYDRYKEHRINGLKAVFVLELMIIFYFFSSIDN
                     PYFYYFYAPITCFTVEAAGTTLKEKYMLLLYTLLGSILSIFLFGIISVYNVLFIFFVF
                     FYTLALYFLVLHHFKKMLVIVPLMLSLASYSLIYGVEADSNFYIAINNALYTLAAMVV
                     IFAGLFIFPKKYYFDIWERAFLEVITGLESLSAKICKGEVKTIAIFSGIIVMERYSKM
                     LPRNIKCYSILKITILSFELILTMSYLLSFQKQLRKEYVIVLHHYLERLCQSCKARQP
                     LILSDQELPAFKETHELEILYQLILSWNYLCADG"
     gene            complement(21636..22709)
                     /locus_tag="ACF2SP_000856"
     CDS             complement(21636..22709)
                     /locus_tag="ACF2SP_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFD2358658.1"
                     /translation="MKEKFATWSKIVTKHIRFFFSYSIPMFFKKAKNYTNLPIIVVGL
                     ALIIGLFHLFSYLIPFTDNAFVVTNVTPVAADVSGFITEIYVKNGQKVKKNDPIFSVY
                     RVPYELAYQQAMADYKEGLKKIDVFQKQIERTVALIKATDAELEKAQFALGLKKNKNV
                     AEAVSVLELKNLGYDVSTLSNKRNALQQEVAVLQAKIEQQRHTVASLKAKMDNAKVNL
                     DLTIVRAPGDGVIDNMYVSPNTPIEIHKPVFSFIDTANYYIQANFNETDLRNVRQGDK
                     AYIILRMYYFDKIFHGVVVNSLWAAERQSTAPKSQIQVVTNQNEWLLLPQRFPLQIKI
                     LDPDPDYPLNPGASAYVYISTKR"
     gene            complement(22811..24022)
                     /locus_tag="ACF2SP_000857"
     CDS             complement(22811..24022)
                     /locus_tag="ACF2SP_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2358659.1"
                     /translation="MSLEVEPKPHGNNPYANRFDNLPSKAQGRFPRVAYVLQGGGSLG
                     AYQFGVVKGLLEAGYEPDWIAATSIGAIQAAIIVGNPPEKRIEKLERFWDEIAPGNFF
                     DFLGESDITLDFYNKLSAACALLFGQPEFFFPKWCTGTIPLWGDPTTLSYYDTTPLRK
                     TLLDLIDFELLNSCPIRLSLGAVQISNGHLIYFNNINYRIEVEHIMASAALPPGFPAV
                     NIDGELYWDGGVHSNTPLEVILEAIPAENTLCFLIDCFGGRSFIPKSMSEIEERRKDI
                     SYGTHAQRTIYNYLQRQKMRNSMMELSRILTDEQKKESAHLLDIGSPHHCTLVHLAYS
                     SRIVKAASKDYNFGRVVIKKRMEAGYSDAKAVLAEESQWGYLPEDGKSRLYESPNNLS
                     RLFRKRKKEVF"
     gene            complement(24459..25298)
                     /locus_tag="ACF2SP_000858"
     CDS             complement(24459..25298)
                     /locus_tag="ACF2SP_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFD2358660.1"
                     /translation="MKAIEVERLIQGTLIREGGGVKLRRYIGADKTNHFEPILLFDYF
                     DSSDPLDYLAGFPPHPHRGFETITYLMEGSITHEDNKGNKGVINAGGVQWMTAGKGII
                     HSEMPSSSTGRLHGLQLWLNLPAAEKWREPRYQEKSSDQLPIEVMESGATIKVIAGRI
                     DQGLSSPIVEIATRPLFLDITLPANATIQQTIPDDYQAVLFVIEGAVNTGGQQITAGT
                     LASLTNGERIQIEGKDKENRNHCLLIAAAKLHEPIVRYGPFVMNTQEEIMQAIHDFRT
                     GYF"
     gene            25444..27021
                     /gene="cegC1"
                     /locus_tag="ACF2SP_000859"
     CDS             25444..27021
                     /gene="cegC1"
                     /locus_tag="ACF2SP_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC1"
                     /protein_id="HFD2358661.1"
                     /translation="MNTTEHTELGNGLLMDKIEGNPYLRIDEFGVLHLRLQRFGENNL
                     PEPMELEMSAGEIIAMAGDYFTQANWTMDLDLPKCELFNSPAELGKHLIRKPIDPKEE
                     SALITAYNNLAAPDVTRKEIDRIYSINNANYVPFSPTLNFYAQQLMYYFRVKDYGEML
                     VRNQTHFTPWSIRVYILGHAIALRYAHLSYELKQLATDRNYQSDNPDLSALKISLQNK
                     NETLSSNTLLDLANRYHAQAYSIELFTFHYYSDHFATGHMSMIGDLRVVLKERFGVWG
                     NILANNLHDEVNRVGVYTVRPYDPTPNATEAPSRARGDGKFDTCLNQFNRLACLNGMT
                     ASLKDINQVLSGSVIPEQKDFGGLVHMPDVDFNSRQHQPLLVLSKGKVYYRNNLSQIH
                     IISPSEYEALRENPEKHGYKELTSKWAAFKLVTKLRLFPYLYDGSVMPVSDEKLAEII
                     ADEKRRNPQRAPIPTPSCLPESEPTVFDWRTKASWRNNKDSLDILDGLKKHSILGAKQ
                     THSAIQEEEEVQLNLGV"
     gene            27188..27658
                     /locus_tag="ACF2SP_000860"
     CDS             27188..27658
                     /locus_tag="ACF2SP_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFD2358662.1"
                     /translation="MKRLIPTFIYLLVLAITPFSTQAEALLKDTFGHNIPFSSLKGKW
                     VFINYWASWCETCVDEIPEFNRFYKNHKNEAVALYAVNYDALPLPEQKLLIKKFNINY
                     PNLLKDPAQDLQLGDITGVPVTFVFNPKGQLIKKLYGGQTAKTLEKVIAENRTS"
     gene            complement(27763..29007)
                     /gene="hflX"
                     /locus_tag="ACF2SP_000861"
     CDS             complement(27763..29007)
                     /gene="hflX"
                     /locus_tag="ACF2SP_000861"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFD2358663.1"
                     /translation="MFERPQGGERAVLVQLALPGVDADKALAEFEELALSAKAEVLDC
                     VLGARATPDAKYYVGKGKAEEIAHLVNMLDADLVLVNHELSPSQERNLERLFGCRVVD
                     RSGLILDIFAQRARTFEGKLQVELAQLQHLSTRLIRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRERIRYINKRLEKVRCSRDQNRQARRKASMPTVSLVGYTNAGKSTLFNVLT
                     GEHTYVADQLFATLDPTMRKLELPGSSAAILADTVGFIRDLPHHLVEAFRATLEETQQ
                     ADLLLHVIDISDPNWRETVFEVQKVLDELKVSNIPVIQVFNKIDLQEGWQPKIDYTED
                     SCKVWLSATTGAGLDLLKEAIATQLHGMILIEDIVIKPDQAKLRAQLYQLGAVLKESV
                     DENGDWLMKIRITADQKRRLLS"
     gene            complement(29035..29292)
                     /gene="hfq"
                     /locus_tag="ACF2SP_000862"
     CDS             complement(29035..29292)
                     /gene="hfq"
                     /locus_tag="ACF2SP_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFD2358664.1"
                     /translation="MSKNHLLQDPFLNELRKEKVPVSVFLVNGIKLHGIIDSFDQYVV
                     MLKNSITQMVYKHAISTVVPSRMVKIPAEESSGEEEGTVAD"
BASE COUNT         9229 a         6061 c         5257 g         8857 t
ORIGIN      
        1 atcagttctc atggataaat tgatttaatt ggtttgcaat tttttctttt ttagcaaaag
       61 aagaatcata cgttgttact aaaggaatag gtggcttatc ctttagagcg atggcaagtc
      121 tataagtttt cgtacctctg ctcccaagcg actcttcgac tattatttta tctacatcag
      181 aaaacgataa tttggtttcc tctgtcttaa acaatccctt tcttttgatt acaattgttt
      241 ttaatattct gtcaaaatca gtaattgctc ctctaacggt aagcaacaca gcacccacca
      301 atagaaaaaa aacggataag ccatacatcc accacgaggc tggataactc actttaaaag
      361 tcgtttccaa gctattgctt atgtagttat tgatgctgtt ggcagcaatt tctttatcgt
      421 cacgcccgct ggaactgctc cccgtcagat tgactgggcc ttgagccgtt aacaaatcca
      481 cataataaag cgtatttcct ttacttgagc gactgctttt aactacggct gcttttaaat
      541 cacctaactc ggtgctggag tggtataaat tataaatttt gctgtgtaaa acacactcct
      601 tagccaaatg gtattgtttg tttttgcaaa cgagggtgta ttccatcatt aaaataagca
      661 ttgtaaaaag acttgcgaca agaaacaaaa ccgcgaacag atatataagc cacatgctga
      721 tgattaggcg catcttgttt gtgctgatag agtgtagttt catttcatat tattgctgat
      781 aacttatttt aaatatagca acaactttcg attatatgta atgattaata ccattagtca
      841 acgtaaaaac cattccacaa tgaccaaaac aaaaccataa gggggctatt tttctactaa
      901 atgctatttt ttaccacaaa taaatctggc ttttaaaaaa aatatgttat tctttccaac
      961 atcactgatt cgtacttaat caaagtggcg aatttatttt ttatcattta acaaaagact
     1021 ttaaatatgg gatggactga tcgattttgg aatgcgtatc gcgtggttac taataccgta
     1081 atcgaaggcg cagcaaactc tttaaatgca gctggatcac tagcttgcct gctcggaggc
     1141 gctggggtct ctctctctta ctccatgtct gaaacaataa aagcagggta cttcggatca
     1201 gttggagtag caggcacagt taatttggat gtgactgcag tagaatttaa ttatggcttt
     1261 aatcaaacca ttccttttcg cagagatttg ctggaaaaat ccggcggtgg cagctatgca
     1321 ttgaatgatt ttgccaatcc aggtactgtt tttatgatta gttctctggc gattctttcc
     1381 ggaaccactt taagaacttt tggggctaat cttaaaaaat ggcaacaaaa cagagaagac
     1441 aaataccact ttattgccaa gtacagcact gacctggaac ccccttctta caaagaatat
     1501 ctgtatgtca acgcagaatc tttcttcaat tctttggcaa tagcctcatt cagcagtctt
     1561 ttggcaaaaa atgttctgga tttttcgatt aaggatttat cgaaatatca tttcacttat
     1621 ccttttaatg gaacaaatat aactggctca gtgaaaacgc ctgattatac tgggccagtt
     1681 acatcagaat ttttccccct tgactttttt gtgtctcaaa atacaaccat ccacacagat
     1741 tatttcaacc tgatagtcaa tgaaaaagtg aaggccaatg caatgaccaa tgtcacttat
     1801 ggtggaggag tacatttaga gcccagccac caatcaagcg ccccccttct ggctgcttct
     1861 gcaaccattg gtatcggtac ctatttggca ggcacttttt ttggcaagaa aatcctgcga
     1921 gaacgtgatg aaagacttca acgttcaaga tcatcgagct attccatagt ttcaggcact
     1981 ttttgggata ataagcatga catagaggca aaaggcgata caaaattcga gcatacccat
     2041 tcattacagt aaagaatagc ctaagtctcc tccatgtaat atattgcaat gaaaaaataa
     2101 aacggagttt ttcatggtat ttaaatcaag gttagttggc gttattttat tgtgcttttc
     2161 aagtattacc ggatggtgtc tttctcagtc ccatgaggag gatagaatca ttgtttcaaa
     2221 ctgcctgtat gagcaattaa aagacaaagc caggttaatt gcgcaatcgg atcactttag
     2281 cttcattgcc attccaattg atgaaaatat tgccaatact ctacaaaaaa tgagaccagt
     2341 ttgtggaaat tatcttaatg ctgagccata tattcaacaa acctacaatc gttttcttga
     2401 ttcgcaaaaa ttattggata aactgacccg ctaccattcc atctcgaccg caaacccata
     2461 cccgattaat catgaagcac aagtacatgg tttgttcgaa caaattgatc cggccaaaat
     2521 atggcaaact aatcagcatt taaccagtta catcaaccgc tctgccaaat ccagaacggg
     2581 agtggaggct gcacaatggt ttaaacagca atttgatacc ttagcccaag attatggaag
     2641 aaaagatgta gagagctact tcgtcaaaac cggaaataaa tttatacaac catcagtcgt
     2701 cactgtaatt ggaaaagata aaccgggaga ggccattgtt ataggggcac atattgacac
     2761 actggacggt aatatgcctg gtgccgatga tgactctagt ggaatatccg ttgaacttga
     2821 aatggcaaga gtgctctttt catcgaactt tgagctcaat agaccagttt attttatagc
     2881 ttatgctgct gaagaaagag gacttatcgg ctctggatat gtggtacaag attttttaca
     2941 gaaaaaaatt ctggtgaaag cagtaatgca gttagaccag gcaggataca gagctaacgc
     3001 caaagaccaa acgatttggc tgttaaagga ttatgtagat aagggtttaa ctgaatttac
     3061 agcagaatta ttaactcgct atgttaaaat tcctgttggc tatactaagt gcggttatgc
     3121 gtgcagtgat catgtgaatt ggaccaatga aggctttaaa accacctatc cgtctgcaac
     3181 tactttagac gatgataatc cttatgttca tacctccaat gatactttgg atattctgaa
     3241 tctggaacat atggttaatt tcactaaact aggcctggcc tttatagtgg agcttggttt
     3301 aaattagcga tgaaagcaat agagcccgtg ccaatttaat ggtgctctgc aattggagtt
     3361 tattaatgaa aacagaagcc ttacttagct ggctaaacgt agaaagcaat gaatcctatg
     3421 attctatcag aataaagtat caactaagac ttgaagcctt ggagaaagag gaacgcatta
     3481 aaaaaatggg gggtatcaat ccttttgcgc ctaaaatacc ccttaacaac accaggataa
     3541 tcacctccaa agaaatacaa gaggcatggg ctgctattga ttctctcagc aagtaccaaa
     3601 aattcaaagg actagtggct caagaagaaa atttatccac tatccctaac aaaaaaatgg
     3661 atagcaagag agaggaaaca aaattaatcc attttgtaag agaacgtgca caaggccctc
     3721 agggaagacg cttgcccgcc catttacaag ctaaaattaa aagagcctct ttggacaaac
     3781 cacacagtaa gctaattact gaaggggtcg aacaacaaat taacgcaatt ttagacaaaa
     3841 ccaccttgtc agcgagtgac ttgcaatctg ctgtttacac cgcagtccag aattatacga
     3901 attatcacat gaaatccgaa aataaaaata atcccaaata caacagagga caaggcgatg
     3961 gttttttcag tttttttcgc catggaagct ttggaatcac tacagcgaat aacctataca
     4021 cttccatcag cgcaaaaaat gctactttag atgaaaccat agaaaaatta aaagattttt
     4081 taggccattc ctccagagca tatcatcatc attctttcac atcttacctg gctgatgcac
     4141 ttgctgagaa gggactatta acatcccatc ctgccagccg gtataaacaa gaagacgtag
     4201 tgaaccagct tgaccaatgg atagcacaaa actcaactgt taatctttta aaatgataat
     4261 ggcaaggata gtctgttttt tactcctgaa gtaaaaacaa ataaattaga atccgccacc
     4321 gacatccgtg ccaaatcttt aaattaggca cggcagctgg tacattttta cttattcatt
     4381 aaaaaaaaca aaaatttatc cccactcaaa ggcttggaaa aataccatcc ttgtaccata
     4441 tcgcagttca ttgagcgcat taactccagc tgttctgctg tttctattcc ttcagcaata
     4501 accgataatt tcagattgcg cgccatagtt ataatactat taatcaaact taactcatta
     4561 tgatttgtat ttacaccctg gataaaaacc cgatcaattt ttaaattttt gaacggatag
     4621 gacttaatat actgcaggaa agagtaaccc accccaaaat catccaaaga aaaatgaatg
     4681 cccatgtcac ttaatttgtt taattgagat aaaatgaatt ttgaatcatc aataaatgcc
     4741 gactctgtaa cttcaaaaat tagagaatct gcagataatt ttgttctttt caaaatgagt
     4801 tgcaccttat gaataaaatc cttttgcttt aattgtaagg tagagatatt caccgctaat
     4861 tttatcttgc tgccggtaat aaattcccac tctttcgctt gtgcgcacac cttatccaaa
     4921 atccaatatc ccaatggaat aatcataccg gtctcctcgg ccaagggaat aatctgatcg
     4981 ggattaattt gacctaaggt tgtattctgc caacgtagca aggcctccgc agcgacgacc
     5041 ttatttttct tcagatcaat gatcggttgg tagcaaatat ataactcatt acgggtaaac
     5101 gcctgatgta attccgtctt aatacggata tggttatttg ccccctcaat catggtattt
     5161 tgaaataccg tccaattatt tttgccccgt atcttgctct gatacatggc tgtatctgca
     5221 ttacgcatta acgttttggc gtccttgcca tgatcaggat atattgcaat tcctatactt
     5281 gtagacaccg atagctcttg tgattcaata acaaaaggct gagaacaaat ttcccggcat
     5341 ttttttgcga tactttcagc atctttatcc tgcatgatgt tttctaaaat catcataaat
     5401 tcatcaccac ctaagcgtgc aatagtatcg gtttgccgta tcatagatag aaagcgctcc
     5461 gatagcgctt ttaataataa atctccaacc gcatgcccta aggaatcatt aatctgttta
     5521 aattcatcca aatctaaaaa cagtaccgcc aatttttgat tgttgatgtg ggattttgaa
     5581 atagctcgtt ccaatttatt aaccgcatag agacgattgg gaaggttggt taaattatca
     5641 aatgttgcct ggtattttaa catttcctcg tagcttttct tctgagtcac atcctccata
     5701 acagccagat aatgagttat ctcttcattc tcattacgaa ttggagaaat aattgctgat
     5761 acccaaaaat gctctccgga tttgttttta ttcagcaact caccatgcca ctcttcccca
     5821 ttggctaagt gttcccacat ggttttataa atttcatgag gagtttggcc tgaccgtaaa
     5881 attcttgtat ttccaccaag caattcttct ttgcgataac ctgtttgctc tatcactttt
     5941 gcattgacat actcaatggt tccagtttta tcagttatta tgactaaaac agggctttgc
     6001 tctaacgctc gtgttaattt tcttatagtt gcttcagagc gtttttgaag tgtaatatcc
     6061 cgtacgatag ctaaaaccaa tttctcccca gtcgcattaa aaactgccaa acgaacgtca
     6121 acaggaaaag ttgtgccatc ttttctgcgg tgcatgcctt ctgtattaat ggggcccttt
     6181 tgaacatttt cccacaactg atttaaactt tcctgagaaa atcccacttc aatatcccaa
     6241 acataggagt taattaattc ttccctgcta taattcaaag acaaacaagc tgcttcatta
     6301 acctctaaaa atgctccctg caaattatgg ataaaaatac catcggatac agcattcatc
     6361 agatttcgat atcgttcttc actgattttt agttgattga atcggtgtac ttgttccgta
     6421 atgtcttgtt taattgctat atgttgggta attctcccca attcatcatc catcacagga
     6481 acgatgatgg tttgagcaac ccaatagctg ccatcctttt ttcttgagcg tatttgcccc
     6541 ttccacgtat tccctgattt aagggtttgg tttatttctt caaacaactg aacgtcttcg
     6601 accggtgaac gcaataagga cgccactgtt ttattcattg cttcacgggc actatacaaa
     6661 gtgatgttct caaaagcggg attaacatat tgaatgactg cctcttcatt cgttatttcg
     6721 accgagtcgc cagctttttc caaaatagtg gtcagcagct tttccctttt tttattcatc
     6781 aatctggcta aagacaggtt taatataccc agaatatctt taaaaaaagg cattagtatt
     6841 tcaaagttac agtcaataga atcaaaccaa aaactaatac aaaataaatt cccttgatat
     6901 ttatcaaggc aaatatcgac acgctgcttt aatttttctt cagaatgctt cttgagcttt
     6961 gtcttatata tttttaaaaa atgccaatcc gagactacag ctaaaagttt ctccaattcc
     7021 tttggcgcat caaattttaa tccattagat gccagaatat taatatgact gccatcatga
     7081 tgccaaactg cggtgattgt gtcttttaat aagggatgct tgccaagtaa tttcaaccca
     7141 cttaagctta aagtatgtag ggtattttca tcataactca attcaatcaa ttggcttatt
     7201 aagcttcgtt ctagcaggtt aagatgactg aaattatctc ctgaaggaat atgatttaat
     7261 ggatgtagct ttttactctt cctcatttta tctatcatgt gccaatttag atctacacct
     7321 atcatttctg tataataaag cttaggatct tattgtgtaa taatcaaaat agatgtataa
     7381 aatttacccc atcaacagct ccatcttaac tcgcagccac caatattaaa ttgccttata
     7441 aaatttaatt gactaatgac ttcaaactac acactaatca agttaacata gagaccttac
     7501 actttatttt taaagtatcc atgcgaacat caagcttcct cgatagatta ataagtgaag
     7561 tcgatagtgc gcttcgaacc ctcgttttgc caccaaaacg tattacaacg cgccaatctc
     7621 ccgctgaaaa tctggctgat actgtgcttt ccgctcagga aaaaaaacat atttcaggat
     7681 taatgagagt aaatcatgcc ggagaggtat gtgctcaagc gctttatcaa ggacaagctt
     7741 taacagcaca acttactcac atcaaagagc aaatggctag tgccgcggct gaagaagttg
     7801 atcatttggc ctggtgcgaa gaacgtttgt atgaattagg cagcaagccc agccttttga
     7861 atcccatctg gtattgtggt tcggttctat taggcgccct ggctggattg gctggcgata
     7921 aagtcagctt gggatttgtt gctgaaacag agcgccaggt aaccgctcat ttacaaaggc
     7981 atctgcatta tctcccggaa aaggataaaa aaactatagc catattaaaa agaatgcaag
     8041 aagacgagga acatcatgcg catacagcga tggaagctgg agcagttgaa ctcccctata
     8101 ttattaagca attgatgaat gctgtatcaa aattaatgac tcagagcagt tattatattt
     8161 aacaggatat gattacatat ggactacaat acgatattca ttctattcgt catatttatt
     8221 gcgttcgtat ttgattttat caacggattt catgatgccg ccaattcaat tgcaaccata
     8281 gtcactacag gtgttttgac gccaagacaa gctgtactat gggctgcttt ttttaacttc
     8341 attgcttttc tgattttcaa tttaaccgta gcccaaacaa taggctacgg actaatcgat
     8401 acggatctgg tggatactta ttttatttta gcggccctga tgggagcgat attctggaat
     8461 ttggtgactt ggtattatgg actgccctca agctcctctc atgccttaat tggcggatta
     8521 gctggtgcgg caatagccaa gggaggcctg tcttcactca agattacagg ttttgccaaa
     8581 gtagcggcag ggatatttat atctcccgtt ctgggattat tgattggctt attatttact
     8641 catattttct ctttgtttct cagacataag aacgaagagg aactgaatac attatttaaa
     8701 agttttcaac tgatatcttc agcattattg agtatcacgc atggaggcaa tgacgcacag
     8761 aaaacgatgg gtatcattgc ggtattactt ttttccgcat cttggcttga tggtgtattt
     8821 tatgtgccat tttgggtcgt tatttcatgt catggggtaa ttagcctggg gactttggct
     8881 ggtggctgga ggattgtgca taccatggga actaaaatta ccaaacttaa caccctgaag
     8941 ggttgtgctg ctgaaacagg agcagctatt accattttcg ctgcaacaga atttggagta
     9001 cctgtctcaa ccacccatac ggtcactggc tccattgcgg gagttgggct aatcagtggc
     9061 ataggtggcg cccactggaa agttttacgc aggatattct actcttggtt gcttaccatt
     9121 ccagcagcag cattagttgc tgccgctatt atgaccatct caccaactgc ctagattatg
     9181 gcgtcactcg gcagcacaat ctccctcaac acttaaggca ctatttgtcc tgctaaatgc
     9241 aaaataaacc gctataccaa ctagcatcca aattataaaa cgcaatatcg ttacccatgg
     9301 caaattcaaa attaaataaa aacagcttat tatacctaat ataggaacat aaggcataaa
     9361 aggtgttttg aatggccttt ccatatcagg atgagttcgc cttaaatata aaacacctcc
     9421 acaaacaaca ataaaggcaa acaaggttcc aatattaacc agctcggcca aggtaccaat
     9481 tggagttact gaagcgagag atgccattaa tatgccacat agcaatataa ttcgaattgg
     9541 tgttttagtg tgttcgttcg ttttggataa gaaccttggc aataagccat cccttgacat
     9601 tgctaaaaat actctggtta atccatagaa cagaaccaac ataactgtgg ttaaaccagc
     9661 aattgcgcct acaccgacca gtccggctgc tgttttataa cccaagacaa gcaggacatg
     9721 actgattggt gatgaaacgt taagagtagt gtaatgagca atcccggtta ataaacctga
     9781 aaccagaata tagatcactg tacaaatagc cagagaagcg attatcccta tgggtaagtc
     9841 ccgttgagga ttaattgctt cttcagcagc agtagaaaca gcatcaaacc ccacgtaggc
     9901 aaaaaagatg attgaagcac ctttcataac accagcccaa ccatagggca tgaaagaaga
     9961 ccaattttca accttcactt caccaaatgc gatagctatg aaaataaaaa taaccaaaag
    10021 tttaacaaac accatgatat tattaaatcg agaactggat ttcacaccaa caattaataa
    10081 tgtagttaaa acagctatga tcaaaatggc taaaatattg agatccccac cgtcagcggg
    10141 accatgtaat aaatgagttg gaataaatat tttcagagcc attaaaaaat cattggcata
    10201 accactccag ccgatggaaa cagccgatac agcaatagaa tattccaata acaaatccca
    10261 tccgacgatc cacgcgatta actcaccaaa acctgcgtaa gcatatccat aggcactgcc
    10321 acaaccccct atgctggcag ccaactcagc ataggataag gcggcaaagg cgcaggcaaa
    10381 tccggcaaca acataggaga atatcacggc tggtccgctt atagtggcag caactattcc
    10441 tgtaagaacg aaaataccag cacctataat cgcgcccact cccaaaaagg taaggtcaaa
    10501 aacactaagg catttagcta aatgtgattc attgtctagc gaccctttta tatctttttt
    10561 acgaaacaag tccatgatag tatgctctca ggtttatttt ttattgttta tttccaaaat
    10621 ttaagttatc aataaacata aactccggtt atcttccatc taacaaaacc ttacacaatt
    10681 aaactctacc attaaattat agctgggaga aaccctgcaa aggttaaaaa ttgaatgaaa
    10741 agattaattt cctgcttaac cattatttgc gctttaaatg cgtccgctgc agcggaaacg
    10801 acatccaatc cttgttctcg ctggataagt ttcctcaagc ccgtctgtca acgcttccac
    10861 caaacttgga cagaaggcca tgatgatatg tatttttcag gctatgcctg gcataaccgc
    10921 tatgtttaca gtaatgaaaa aattaagtcc tacaatgaaa ccgcatgggg tggtggatta
    10981 ggtaaaagtc tttttgatga aaaaggaaac tggcatgggc tttatgcgat tgccttcctg
    11041 gattcccatc gccatttaga gcccgccgtt ggttacgcct atctcaaaac agcttcagtc
    11101 aataaagatt taaaagcagg ccttggatac tcggtgttag ttacctctcg ggttgattat
    11161 gataatgtcc ccatccccgg agcattaccg tgggctgctc ttttttataa aagaataact
    11221 atcgcagcaa cttatatccc tgggtccagt cgtgaaggac atgaaaatgg aaatgtattg
    11281 tatatgctcg gaaaaatatc tttataaaga tggattaact tttgctagct gcaaaaggtc
    11341 atgtgaaaca aacccggatt agatcccttt aaaacggttg caactgaaaa ttcaacttgt
    11401 tagtcttttg aagattttta actgctaagt aacaaggaga agtaacatga tgttgaaaac
    11461 ccagttgact gcttttatcg gtgctgtaat cttggctggc tcttctttag caaatccaat
    11521 aaaacctgaa gtatgcccca gtgtaccctc tattcaatcg gaaggaatgt ccatgtcttc
    11581 tgaaattttg gagggcatgt acatcaccta taatttaagt cattacaata ccagttcaag
    11641 ctgggtgttt atcgtagggc caatcgcagc tgaaaatgat gatatggcat tggcagaaag
    11701 caataaatta ctttcaacca tgtcagggtc tccccatccg gaagatgatg gagaaggcaa
    11761 ttggatatgt cagtatacga ccaaatccaa agatattatt gcatttgcca tagaagcaga
    11821 tgatatgctt tctccattga aaatgatgag atatctcaga acaatccgct gataagtgga
    11881 taacacctgc acggacacgg aagcatgatt tgcttccgtg atttaacgaa tagttaagaa
    11941 ctcttcctct ctttttggta cttctttatt ttatgtgcta tgttttcttc atgaccctaa
    12001 cttcaggacg aaaagaaaat gaaatcgttt attgagcaag ctcaattcta tgcaacctac
    12061 caccaaaacc aggcgacacg ttatacacat atggcaggag tcccgttaat catgctttct
    12121 atcatgattt ttctgggatt tgttaaaata gttattccag gtgtttatgc aaccgatctt
    12181 gcttgtttgg caacgcttgc cgccctcatt tattacttcc tgttaaactg gcaacttgcc
    12241 ttggcattaa ctcccatttt gatctttttg ttatggctgg ccagttggtt taactatgat
    12301 ggcccaacga aattcggact ttgggtattt attattactt ttgttgtagg ctggggattg
    12361 caattttacg gtcattttat tgaaggaaaa aaacctgctt ttatggttaa ttttacccaa
    12421 gcccttattg cgcctttgta tttaacagcg gaattgttct ttatggcagg actcatgaat
    12481 tccctgagag aacaaattta cgggaacgaa gaagtaaata ctgaagaaat aagcaaaagc
    12541 aaaaccaaaa caaaatccaa atcttaaaga gagtcgagct tcacgcttat gaaagggaag
    12601 ctcggcaaga gatctaataa taaataactg aattattcag ttatccgcaa aatgcggcat
    12661 tacaatttta aaacaatggt gaattttgct atttcgttta aaatcctgat cgatggtctg
    12721 tggtgtaatg tcttgtactg catatttctc ttttagcatt ggctctaact taaattggcg
    12781 cagattagtt gagaaatata aaacaccatc aggatttaac aaccgcattg ccatgttaat
    12841 caaagaaaca tgatctcttt gaatgtctaa aatatcgctc atacgctttg agtttgaaaa
    12901 gctgggggga tccaaaaaaa taacatcaaa tttatccctg gtagttttca tccattcctt
    12961 acaatcgtat tgtaaaaatt gatgcttgga taaattaata tcatttaatc taaagttatc
    13021 ttccgcccaa agcaaatacg ttttggataa atcaacattc gtggttaatg caccggccaa
    13081 ggcagcatgg acgcttgcac tggcggtata acaaaaacag ttcaaaaatc gagttcccgg
    13141 ctctaattga gcaaatttca agcgcataag tctatgatcc agaaatagtc ctgtgtcaag
    13201 ataatcatac aaattcacta tcaatttcgc cttgccttct gtcactatca atctttggct
    13261 ggttttacct attttttgat attgttctga gcccttttgc tgctttcttt gtttaactat
    13321 taatttttca gggtgaatac ctaaggcccg tggaacgact tgcaacatct caagactgcg
    13381 tttttctgcc ttgtgtactg gaatgctggc tggtggcgca tattcttgca gtacagcata
    13441 gtcattatat atatctatgg cataggcata ttcgggtaag tccgcatcgt aaattcgata
    13501 acaggtgatt tgattttttc ttgcccattt ctgtaaatga tttctgtttt tttccaatcg
    13561 gttaaatagc atttgtgcat tatcagaaag cggggcatcc ggggtatttt ttagtttatt
    13621 tgcagcagaa agagtcaaac aatagagttt gcactctaat gggccattat ataaagtata
    13681 ttgcttatca gctcgtaatc ctaatgcttt agccaaaact gggctggaag ttaaaatagc
    13741 cgcttgccaa ccttgataac aagtatgtaa tgtagtgccc aattgttgat ataaaggaac
    13801 aagttgagtt acctccccca atcgctcacc gtaaggtgga ttacaaacaa ctaatcctct
    13861 ttttgttcct ggcctacaag catttagcgg caatgtatta aattcaacca aaggtaatac
    13921 gcctgctctc tcagcattgg aacgcgcaag agtaattatt ttgccatcag cgtcagtacc
    13981 aaccagctta actgccagcg gtttcacttg ctgtaatgcc tgagttcgca atttttccca
    14041 caaagaactc tgatgccgag cccaatattg taaggattga tcttgtcgta ataaacctgg
    14101 ggcaatatga gctgccatca tggctgcttc tataactaat gttccagagc cacaaaacgg
    14161 atcatgtaag ccatatccct gtgcggctag ctctggccat ttggccctta ataacatggc
    14221 tgctgcaacg ttctccttta aaggagcctt gcctgctttt ttacgatatc ctctttgatg
    14281 caggctatat ccaaccaaat caaaactcac cgttaaaata tcatttttca aatgagcgtg
    14341 aattaaaatt tgcggttttt ctttgtcgac agaaggcctt gacccattca agcggcgaaa
    14401 atgatccact attccatctt tgactatctg agcgccaaac atggtattac gaatttgctc
    14461 agaggcacca tgaaattcaa tggctatggt tttatcatgg gtaaaaacag tttgccaatg
    14521 aaaatcggta cataactgat gtactgcttg ctcttttgcg gcgtggccac taaataaaat
    14581 aagctgtatt ctgttagcaa gtcttgacca taaacataaa ttataaatta caggcaggct
    14641 agcctcacca tagacacctt gaggactcac ttgagtcaca tgaagcccca atcccttgag
    14701 ttcgtcttcc agcaaatact ccaacccttt tgaacaacta atgaatagag agtaattcat
    14761 tttattaaag accttaaata gcgaaacagt gctttagcag cacccgtatt tttctctttc
    14821 atctgatcat ctaatgcctt tttaatcagt tgccttaatt gttgcacgtc ctcaggttga
    14881 taagcatcga taaactctgt caaagcgtct tttccatctt gtaataacct gtcgcgccat
    14941 agctctatct catgaaaaca ggccgtaatt gaactctctt cttcaaggat tttctcatag
    15001 gcagcaagaa tggcttcaaa atcggctgca cgcattaact tgcctattaa ttgtgcctgt
    15061 cgccttttag ccccatggct tttgattgac ctggcatcaa taattgcttg acgtaaatta
    15121 tcgggaaggg gcaaagaatc cagtttggat gcgcttaaat caataaattt aatgcctata
    15181 tcttgtaaat aatgagcatc tctttttttc tgtgatttac tgatttgttc atccataggg
    15241 cgatatattt attaaaataa cgttaattat actatataag tacctatcca gataggatag
    15301 actctaatca attttgaata aaaacaagca aataccaaca tcaacctgca tcatcctatt
    15361 agttagccat agctttaaaa gcgctgttcc cgaaaaacaa taaagaaaac ttataaactg
    15421 agagactcaa tccgctgccg caagtttaac agatgattgg acgtattgtt tgacaggtct
    15481 tgatcgtaaa taagtatgac caattgctaa aaaattatag ataaggataa ataacagtaa
    15541 aaaatgcagc caggttattg ttaattttgt aaaaattgga atggtgatca taacacccag
    15601 acatcctgaa aagcttaaaa tcaaagtctt ttttaacggg attttatttt tttgtaaaaa
    15661 aactaatgtg gtcagcggct gttgcatggc tcctgcagtt gtcatgatag gaaaagctac
    15721 aactggtgaa acattcatta aaaataaaac accttgcacc atgacaaaaa gccctactcc
    15781 gacagaggta agagagccac acactaccat agcaaaaaaa ccaataacca gcttccagga
    15841 atgcaattct gtcaaatcac cacctactgg caatagacgc aattgatgag cgattaaaag
    15901 aacaatgatt agggcaaatg aacaaatcat tgccagtcta atggcttgtt tgcttaactg
    15961 gctcactaca aaaccaccta ctaacccacc aatacaggtt ccggtaacta aagtaagtag
    16021 cgtagttaaa tcaacatcta ccatgtgtat gaaaaataaa gattcgatag cgccagggag
    16081 gacttgagcg ccattattta cagcaggtaa ttcatcatcg cgaaaggtgc ctaataattt
    16141 tgccaaggct acattaacag cgaaactgcc gacccccact gtatctgcaa taaaagcaat
    16201 aactccgcta cacattaatt tgacatattg ggagaaggat agaggcaagg aaggctgctg
    16261 cataaatttt aaaaccatta cggccaagca gaaaaaactc aatattatta tgcaaagcgt
    16321 gattaaaaaa aatgccattc tgttcctaat acgaaagtaa tatataatcc caatgatttt
    16381 aaaatcaaag cttattaagt tttctattcc aaagaaatct aaaagaaaga tcctgtttga
    16441 ttatatacta atttctatgc ccagcaaaca acactgcgag caaaaatagc tcgtgatgaa
    16501 ccacaagaga tcaccgcaat ttgttggagc aaaaacttgc cgggagcttt tgcccccgct
    16561 aaatagcaca cccggctata tcaataccta cttgaccaaa agcagactta accttattga
    16621 tatcagtatt taaatccctg gcagcataaa tgactccgca agctccattt ttaaagtctg
    16681 tcgtaggagt ccaatatttt gtgttagcaa caaccatgat gtgataagcg tttttaatac
    16741 ccatatcctt agacattaag taaaaagcct tattgaacac accactggct gtatgaacta
    16801 tgaagctctg gcgttcttga ggaagtgcga tatgtgcatt ggcataggct actacctcat
    16861 cataattgat agcacaatag ctgccattgt tttgcgcaat acctttgtct aaacaatctg
    16921 cggagcttcc atcagaggat gggtaatcca tgaatcgcaa agctttgcca taagaatcac
    16981 gaactattgt ttctccaata ccccatgtga cttcattggg ctgtaagtaa gctttgttat
    17041 acaactgagg atttttttcc aaaagataag ctcttgacgc ttgtcctgcc atatcagata
    17101 gggactcatt aagtgcacct gattgatcat gatactcaag accagaatgc tgctctgtaa
    17161 aaccatgagt cacttcatga ccggctacat ctaaagaaac caatgggtaa aaatccaacc
    17221 catctccaaa tgacatagct tgtccatccc aaaaagcatt atcatagttt tgcccaaaat
    17281 gaactcgcat aaccaattgc attggagcac cattagaatg ttgtaaggca ttaagtccat
    17341 accagtcttt gtacatatca acaataacat gtccgaaata atacgcatca ttaccaggag
    17401 aaaatcctcc attaatattc tcttctatat tattgttaca aacgtattca aaaggagtat
    17461 ttatcgtgtt ttcccaatcc cattgagaat ggagattaac caacttgact tttgagtttt
    17521 ccatgacgca ttggctgcca ttttgagtca catccaaagc aggcaatcca tctttaccat
    17581 accaatattc ctgaactttc tcattgcctc caggccctgt atccaaataa tttttgatat
    17641 tgttccattg cttaatcaaa gctcctgttt gagcatcaat aacaaaaaaa ggccagtctg
    17701 gtttaccatc ctgttgcacg cttttaaatg aaaccaaata aaccagcttt aattgattat
    17761 tatggtctgg ccgaatctgt aattcagctg tttcctgttg tatgttagat tgggggctaa
    17821 attgaaaata ggattttttt gcatattcct tcgcttgttg cgcactaata gccggtttcg
    17881 tattaagttc tatattatcc aataaatggc cgttcacctc tgcattgtca ttggactgca
    17941 ctcctgagtc tgttccttta gtaatcatca cttgggcgcc aataacaggt attcctttat
    18001 acagttgctg ataacgaaca atcatttgat tatcttcttt tgtttgattt aatttttgca
    18061 atgaattatc aataacggca gaagaactct ttaaaataat cgcattcttt ggtgtttgta
    18121 gtatagggta tttattgatg ctattgagag gggcttgata caaatctaca ttgtcagcag
    18181 caaaaatact tgaagtagat atcgacaaaa taaaaaacat tatttttttt aacatacaca
    18241 actctcacag aatacacaat ggcgcaaata atgtcacaaa attaaacatt atgcaatatt
    18301 tatttgcata tcttgccttg atatgtgcgt atccatactc accatgtatt aaaaataacc
    18361 acaaaagact gaatcaggga ggcattgtag aagaagataa gctgggtact atgaaaaatg
    18421 aaatacgccc ctattcgtaa ttaataattt ataacagatt tctgtttgct cagactgaag
    18481 actttatttc agactctaat ttggcgcgat aacctccagc cagttcttga tataaacgta
    18541 caatgcttag tagctcttgc aatttatcct gatttaaact gatgttgatt ttatcgagcg
    18601 aaattttgct atttaaggtt tctatataac tttgaatacc tgtctgatat aacctgtcgt
    18661 tcaaattata cgcttttgcc aaatgttttt gagccgacgc agtttgtttg agcttattgg
    18721 ttaaacgctc attttctgat aaggcattgg tcgtatctct taatgctttt tgtaaagtat
    18781 caatataatt ataataagaa accttattta cgcctttagc cttagctact gcccctaaaa
    18841 caggcatatc aataatgggg gctttcaaca attgatcatt aaatttcacc gtttttttcg
    18901 gtaatgcgta gcggctatct ccagcagcta aaccaccaat ataatctaat tgtatagtag
    18961 gtaaaagatt actttctgca acgccaattc cttcatgtga agcacgcaaa cgattttcag
    19021 ctacttgcat atcaggccga ttttcaagaa ctgttaacgg taaagaaccg ggtattaatt
    19081 tatgattttt aagttgtaat aattttttaa ctgtttttat ttcccctgga ttttggttta
    19141 gtaaataccg gagagcattg cgacttataa cgatattacg ctctgttacc tcgagttcgc
    19201 cttgtatcaa attgacctgg ctatacaact cttcctgctc aatttcagaa cttaatcctc
    19261 cttggtaaac tttactggca acctttgcca actgagttag atcattggct aatgtctgta
    19321 aaaattcttt gcgttctatt tctgcaagat aggtgaaata acttgccgtt atttgagaaa
    19381 taattgttag tttgagagca tcgtcttctg cttttacctg tgcaagagta tattttgccc
    19441 ttttttgctc tcttatttga ttgatgatgt ttattgtata gttaggaata aaaacaatca
    19501 gaacgccagg aaaacccgtt gccggatttc ttgaataacc agtcataaga tcgagggtag
    19561 gcacccactg ataacgaatt tttttaagct caccctctgc cgcttcaatg ctccctcttg
    19621 acatgccaag gcttgtattg gatatcaacc ctctttcaat caactggttg agagtggggt
    19681 ctttaaaatc acgccaccag gctatgtaag gaatattctt gctatcgata tttttaacat
    19741 tcttgtcttt taccgaccat ttattaatca cttcaacatg gggtctgtga taaggctcac
    19801 tataacatcc gactaaaaag actgataata aaatgatttt aaccggacgt agcatgcttt
    19861 actccctcac taagatcaat tgtttgctcc cctgcaacaa aatcttttaa gtgcaccaaa
    19921 tctttttgaa cctgttgaaa tagttgataa taatcaaacg tatgataatg tttcgtataa
    19981 taagccatat ataaggcttt caggccaatg aaaatacgat tagccaattc cacaaactga
    20041 ttaaattcat ccccgacagc atgttctgaa ctgattaaaa actgggagtt tgttatgagc
    20101 ttattgacct caaacaaact gtcagtcaat ttgatacaag cctggaacca ttctgttttc
    20161 tttattttac cctgatttag caaacgaacc agcttgtaaa gatcatcaat cacttcagcc
    20221 tgagtacttt tgataaatcg ctgcatcagt ccgtaatgcc taaaccaaaa gtattcaaag
    20281 cctagaataa tagtaaaggc gatcaacgtg caaagagtat aatcgataat aaaataggtg
    20341 actgggagcg tatcttccgt actaaagtaa ccagaaccaa gtactgacac attgaccgta
    20401 aatacagtgg gaatactgta tgctcttcct accagaaaaa acgcgaaaaa aaccattaaa
    20461 ggcataatca gaattaacgt tcgataatcc agatggccta caaaccataa tagatatccg
    20521 gtgaataggc ctaaaagcac tcctaaaaac cgatgataag tcctgaatat agttgttcca
    20581 ttatcaaaac cggcataaat catcatgaca gcaaagcccg tccacccggc ccgcggataa
    20641 cgcaaccatt cctggacaaa aatagtaaac gcaaagaccg tactgatctg aatagagcgc
    20701 agctcaacat aagaaaatgc ctgtactcct aaccatcggc gcataagtaa ttccagctta
    20761 aaattaattg atacaaaatt tccaactcat gcgtttcttt aaatgcaggc agctcctggt
    20821 ctgagagtat cagtggttgc ctcgctttac aggattgaca aagacgttca agataatgat
    20881 gtaaaacaat aacgtattcc ttacgcagtt gcttttgaaa agacagcaag taagacatgg
    20941 tcaagattaa ctcaaaagat aaaatggtta tctttaatat actgtagcac ttaatatttc
    21001 ttggcagcat tttggaataa cgctccatca caataatgcc cgaaaaaata gcaatagttt
    21061 tgacttctcc cttgcaaatt tttgcagata gagattccag gccggtaatg acttcgagaa
    21121 aagcacgctc ccaaatatca aaataatact ttttgggaaa aataaataat ccagcgaaga
    21181 ttaccaccat ggctgccaag gtatataaag cattattaat agcaatataa aaattgctat
    21241 ctgcctccac accataaatc agggaatagg acgccaaact tagcatcaat ggaacaataa
    21301 caagcatttt tttaaaatgg tgaagcacga ggaaataaag agccaaggta tagaaaaaaa
    21361 cgaaaaaaat aaaaagcaca ttgtatacag aaattatgcc gaacaagaat atagagagta
    21421 tgcttcctaa aagggtatag agtaaaagca tgtacttctc ttttagggtt gttcctgccg
    21481 cctccacagt aaaacatgtg attggggcat aaaaataata gaaataggga ttgtcaatgg
    21541 aggaaaaaaa gtaaaatata atcatcagtt ccaaaacaaa tactgctttt agtccattaa
    21601 tacgatgttc tttataccta tcgtaggatg cgagcttatc ttttagttga gatataaaca
    21661 taagcactgg cgccaggatt taaagggtag tcaggatcgg gatccaatat cttgatttgt
    21721 aatggaaatc gctgaggaag caatagccat tcattttgat ttgtaacaac ctgaatttga
    21781 ctctttggag cagtgctttg tcgttcagca gcccataacg agttgacaac aaccccatga
    21841 aatattttgt caaaataata catacgtaaa atgatatatg ctttatcacc ttgccgtaca
    21901 ttgcgtaagt ccgtttcatt aaaattagcc tggatataat aatttgcagt atctataaaa
    21961 gaaaacaccg gtttatggat ctcgataggg gtatttggag agacatacat attatctatc
    22021 actccatcac caggagctct gacaatggtt aaatcaagat taaccttggc attatccatt
    22081 tttgccttaa ggctggcaac agtatgtctt tgttgttcaa ttttggcttg caaaaccgct
    22141 acttcctgtt gcagagcatt tcttttatta cttaaagtag aaacatcata accaagattt
    22201 tttaattcaa gaacagatac tgcttcggcc acgttcttat tttttttaag gccaagagca
    22261 aattgcgctt tttcaagctc agcgtccgtt gctttaatca aagcaaccgt tctttcaatc
    22321 tgcttttgaa acacgtctat tttctttaat ccttccttgt aatcagccat tgcttgctga
    22381 taagccaact cataaggaac cctgtaaacg ctaaatatag gatcgttctt ctttactttt
    22441 tggccatttt ttacatagat ttcagtaata aaaccagata catcagcagc tacaggagta
    22501 acattagtaa caacaaacgc attgtcagtg aaagggatca aataggaaaa taaatgaaaa
    22561 agaccaataa taagagccaa gccaacgaca ataatgggaa gattagtgta atttttggcc
    22621 tttttaaaaa acatagggat agaataacta aagaaaaacc ggatatgttt agttactatt
    22681 ttagaccatg tggcaaactt ctctttcaca atgcagcgcc tctttgatga tgactctata
    22741 atacaacatc agtttatctc tcaaagagag aaaccccatg acaaggccct cccctttttc
    22801 ggcattaaaa ctaaaatacc tcttttttcc gttttcgaaa caatcgagaa agattgttgg
    22861 gtgactcata taatcggctt tttccatcct caggtaaata accccattga gattcttctg
    22921 ccagcacagc cttggcatct gaatacccag cttccattcg cttcttaatg accactcggc
    22981 caaagttgta atccttcgaa gccgctttta ctattcttga actgtaagcc aaatgaacca
    23041 aggtacaatg atgagggctt ccaatatcta acaaatgagc gctctccttc ttttgttcat
    23101 ccgttaaaat acggcttaat tccatcattg agtttcgcat tttttgtctc tgcaaataat
    23161 tgtaaatagt acgttgagca tgggttccat atgaaatgtc ttttcttctc tcttcaattt
    23221 cactcatgct tttgggaata aacgagcgcc caccaaaaca atcaattaga aaacacaagg
    23281 tgttttctgc aggaatggcc tccagaataa cttctaatgg ggtgttagag tgcacaccac
    23341 catcccaata caattctcca tcgatattaa cggcaggaaa gcccggaggt aaagcagcac
    23401 ttgccataat gtgctccact tctatacgat aattgatatt attaaaatag attaaatgcc
    23461 cattgctgat ttgaacagca ccaagactta agcgaatggg gcaggaattt agcaattcaa
    23521 aatcaataag atccagcaat gtcttgcgaa gaggagtcgt atcataatag ctcagagtcg
    23581 ttggatcacc ccataaggga attgttccgg tgcaccattt tggaaaaaaa aactcaggct
    23641 ggccaaataa taaagcacaa gcggcactta acttattata aaaatctaaa gtgatatcag
    23701 attcacctaa aaaatcgaaa aaattacccg gggcaatttc atcccaaaat ctttcgagtt
    23761 tttcaatacg cttttctgga ggatttccta caataatcgc cgcttgtatg gcaccaatag
    23821 aggttgctgc aatccagtcc ggttcatacc cggcttcaag aagcccttta accacaccaa
    23881 attgataggc tcccaaagat ccaccgcctt gcagcacata agccacacga gggaaacgtc
    23941 cttgagcctt ggagggtaag ttatcaaagc gattggcata aggattgtta ccgtgcggtt
    24001 taggctccac ttccaaactc ataaaaactc ctttttcact ttagttgacc atcgcatcct
    24061 ggctgaaatg ttgggaatac ttgctgccgg ttaataacac tgttcaaaat tagattgata
    24121 tatgcatcgt tattaaataa cgtgaattat ggataaggat tatataagaa gctgtcgata
    24181 tcccgggttc gttcgggctg aggagatgcg taagcatcgt ctcgaagcct tggcacgaaa
    24241 cattaaattc catacaaggc ttcgagacgg cataaatgcc tcctcagccc gaacgattca
    24301 agataacgta tccataattc acgttaaata agtttcgcag aaatcataag tgtttaatca
    24361 ttcacattgt actcataatc ctttgagcaa ccaagataat atatgatgat cattctgaat
    24421 agttttaatt atccgactaa aatcaacaca ataagccact aaaaatatcc tgtacgaaaa
    24481 tcatgtattg cctgcataat ttcttcttga gtattcatta caaaagggcc atagcgaaca
    24541 ataggttcgt gaagtttagc cgcggctatc agtaaacaat gatttctatt ttctttgtct
    24601 ttgccttcta tttgtattct ttcaccgttt gtcaggctgg ctaaagtgcc tgcggtaatt
    24661 tgctgtcctc ctgtattcac agcaccttca atgacaaaaa ggacagcttg ataatcatca
    24721 ggaatcgttt gttgaatagt tgcattggca gggagagtga tgtctagaaa caaaggtcgg
    24781 gtcgcaattt caacaatggg tgaactcaat ccttgatcaa ttcttcctgc tattactttg
    24841 atggttgcac ctgattccat gacttcaatg ggtagttgat ccgatgattt ttcctgataa
    24901 cgaggttctc gccatttttc tgcagcaggc aaatttaacc acaactgcaa accatgcaac
    24961 cggcccgttg aagacgaagg catttccgaa tgaataatcc cttttcctgc agtcatccac
    25021 tgcacaccac ctgcgtttat tactcctttg ttacctttat tgtcttcatg ggtaatgctt
    25081 ccctccatca gataggtaat tgtttcaaat ccgcgatgcg gatgaggcgg aaacccggcg
    25141 agataatcca atggatctga actgtcaaag tagtcaaaaa gcagtatagg ttcaaagtga
    25201 ttggttttat cagctcctat atagcgacgt aatttaactc ctccaccttc tcgtattaat
    25261 gttccctgga ttaaccgctc tacttctatt gctttcatct taattactca ttaattactg
    25321 tcttatatta agatagttca aaatagtacg gtttcctaat tgaattacta ttatttaaac
    25381 tattggcacg ttcagttatc aaggatatga tgaaaataga ctctaaggat aggagcagaa
    25441 tttatgaaca caacggaaca tacagagctg ggcaatggtt tgctcatgga taaaatcgaa
    25501 ggcaatccat acttgcgaat agatgagttt ggcgtgctcc atctgcgact tcaacgattc
    25561 ggtgaaaaca acctgcctga acctatggag ttggagatgt ccgctggcga aattattgcc
    25621 atggcgggtg actactttac gcaggctaat tggactatgg atttggatct gcccaaatgc
    25681 gagcttttta actccccggc agaattggga aaacacctca ttcgcaagcc tatagatcct
    25741 aaagaagaga gtgctttaat tacagcatac aataacctgg ctgcccctga tgtcactcga
    25801 aaagaaattg atcgcattta ttccattaat aatgctaact atgtgccctt ttcgcctacg
    25861 ttgaatttct atgctcaaca actgatgtat tattttcgtg tcaaagacta tggagaaatg
    25921 cttgttcgga accaaaccca ttttacgcca tggtctattc gagtttatat cctgggtcac
    25981 gctatcgcat tgagatatgc acatttatcc tacgaattaa aacaattggc taccgacaga
    26041 aattatcaat cagataaccc tgacttgagt gctttaaaaa tctccttaca aaataaaaat
    26101 gaaactcttt caagcaatac cctgcttgat ttggcgaatc gctatcacgc ccaagcttat
    26161 tctatagagc tatttacttt tcactactat tctgatcatt ttgctacagg acatatgtcc
    26221 atgattggcg atttacgtgt cgtattaaaa gagcgcttcg gagtatgggg caatatcctg
    26281 gctaataatc ttcatgatga agtaaaccgt gttggagttt atacagtgag accctatgat
    26341 ccaaccccta atgcaacaga ggctccgtct cgagcgcgtg gggatgggaa atttgatacc
    26401 tgccttaatc aatttaaccg tttagcttgc ctaaacggta tgaccgcctc actaaaggac
    26461 atcaatcaag tattatcagg aagtgtaata ccggagcaaa aagatttcgg tggtttggtg
    26521 catatgcccg acgttgattt taactcaaga caacatcaac cattgctggt tttaagtaaa
    26581 ggaaaagtat attaccgcaa caatctcagt caaattcaca taatttctcc ttctgaatac
    26641 gaagcactca gagaaaaccc tgaaaaacat gggtataaag agctaaccag taaatgggct
    26701 gcctttaaat tagttaccaa attacgcctc tttccttatc tttatgacgg ctcggttatg
    26761 cctgtttctg atgaaaagct cgccgaaatt atcgcagatg aaaaacgcag aaatccacaa
    26821 cgagctccca tccccacacc atcatgcctt ccggaaagtg agccaactgt attcgattgg
    26881 cgaacaaaag cgagctggcg taataataaa gactcactgg atattttgga tgggttaaag
    26941 aaacacagta ttttgggagc aaaacaaacc cattcggcca ttcaagagga agaagaggtt
    27001 cagttgaatt tgggagtctg attatttcct gataaactgg agctattatt actatttaat
    27061 ttttaataac ccatttgatc tgtcctaaag ttagtaaatt ggacataagc aaatacttgc
    27121 attttcttat gtccaatttg gctaacaata ataaccgtaa acaattttga ctattcaaga
    27181 ggtgatcatg aagcgcttaa tacctacttt catatacctg ctggttttag caattactcc
    27241 cttctctact caagcagagg cgcttttgaa agacaccttt ggtcacaata ttcctttttc
    27301 atcacttaaa ggaaaatggg tttttatcaa ctattgggca agctggtgtg aaacctgtgt
    27361 tgatgaaatc cctgaattta atcgctttta caaaaaccat aaaaatgagg cagtagccct
    27421 atatgcagtc aattacgatg ctcttccctt accagagcaa aaattactaa ttaaaaaatt
    27481 taatattaac taccctaatt tgttaaagga tccggctcag gatttacaat taggagacat
    27541 cacaggagta cctgtaactt ttgtatttaa cccaaagggg cagttaatta aaaaattata
    27601 cgggggacaa acggcaaaaa cattagaaaa agtcattgca gagaacagga cctcctaaga
    27661 gaataaataa aaattatatt tttggtcaat aagttaaaga ttatcttgtg aatttatcaa
    27721 gtctggaatc gattctatcg ctccgcagcc tgacttgatt attcatgaaa gcaatcgtcg
    27781 tttttgatca gccgttattc taattttcat taaccaatcg ccgttttcat ctacagactc
    27841 tttaagtaca gcgcctaatt ggtataattg ggctcgtaat tttgcctggt caggcttaat
    27901 tacaatatcc tcaattaaaa tcataccatg caattgagtt gctattgcct cttttagaag
    27961 atccagacca gcgccggttg ttgctgataa ccataccttg caagaatcct cagtataatc
    28021 aatttttggt tgccagcctt cctgcaaatc aattttatta aacacctgaa ttaccgggat
    28081 attactaacc tttaattcat ctaacacctt ttgtacttca aaaaccgtct ctcgccaatt
    28141 cgggtcagaa atatcaatga catgcagtaa tagatcagct tgttgggttt cctctaaagt
    28201 agcgcggaat gcttctacca agtgatgggg aagatctcga ataaacccta ctgtatctgc
    28261 taaaattgct gctgaggacc ctggcaattc caatttacgc atcgtcggat ccagggttgc
    28321 aaacaattga tcagccacat aagtgtgttc ccctgttaag acattaaata aagtggattt
    28381 tccggcattg gtgtaaccca ctagagaaac agtaggcatg gacgccttcc gcctggcctg
    28441 cctgttttga tcgcggctgc agcgtacttt ttccaatcgc ttattgatat aacgaatgcg
    28501 ctctcttaac aaacgccggt ccgtttccaa ttgggtttct cctgggcctc ttaaaccaat
    28561 cccacctttt tgcctttcca aatgggtcca tccacgaata agccttgttg ataaatgttg
    28621 caattgggct aactcaactt ggagtttacc ctcaaaagta cgagctctct gagcaaaaat
    28681 atcaagaatc aagccacttc tatcgacaac tcgacatcca aataatcgct cgagattccg
    28741 ctcttgagaa ggcgatagct catgattgac taacactaaa tccgcatcga gcatgtttac
    28801 caaatgagct atttcctcag ccttaccttt ccctacataa tatttggcat cgggagtagc
    28861 tcgggcacct aatacacaat ccaacacctc agccttcgcc gaaagcgcta actcttcaaa
    28921 ctcagccaat gccttatcag catcaacccc ggggagagct aactgcacca aaacagctcg
    28981 ctcaccgcct tgtggacgtt caaacactac cctaatcccc aaacaataaa ttgattagtc
    29041 tgccacagtt ccttcttcct caccagagct ttcctcagca ggaattttga ccattcgaga
    29101 aggaacaact gttgaaatgg cgtgtttata aaccatttgg gttatggagt ttttaagcat
    29161 caccacatat tgatcaaaag aatcgattat cccatgcaat ttaataccat tgaccaggaa
    29221 cactgaaaca ggtacctttt ccttgcgcaa ttcatttagg aaagggtctt gtagtaaatg
    29281 atttttagac attgcctact ccttgttgtt attgtaattt ttaaaggcaa aaacgggtat
    29341 ctaactattt attcgacacc ctataataaa actttagtat attttcatga cagcgtggtt
    29401 tgaa
//
LOCUS       DAXLDT010000024        28855 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXLDT010000024 DAXLDT010000000
VERSION     DAXLDT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 28855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28855
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.024"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            172..1149
                     /locus_tag="ACF2SP_000863"
     CDS             172..1149
                     /locus_tag="ACF2SP_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HFD2358665.1"
                     /translation="MKLDVELYINARKQIEKKFQLLPYGPRLIIYELLNLANPLTGIV
                     SDISYRDLSKALEIKSAPGRINSGIPSKQTIRNFIKSIEHECGEYFKVITEGQNLKFM
                     FPEAPQIYNQLIRKKEVNTDLNSPKSLMDSDIEGKLEVQSNIEVNREANTLKSPAKNI
                     KIFNITNSNKQTQTANNDFCCSKKQIHDDFYPNPKTIEMAFSMGLTKVTDLKEIQAFI
                     KHNKKHNTQWADFNPVFINWLERDSQYTQNKQQKAQGQLRSHHNERGTNQSTLNQTAL
                     ERVSEHHGISIDSLWDTQDSELNSGAFIEGTLVQPLDEADRNIRATFYQ"
     gene            1157..1630
                     /locus_tag="ACF2SP_000864"
     CDS             1157..1630
                     /locus_tag="ACF2SP_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358666.1"
                     /translation="MKDTGVWFAALKDLTPKALDSGVERLMALNKGDKFCEFPPNCLQ
                     FRALCLGFYSDLRLPSAAQAHREVLNSAYSTNPNWSHAVVKFTAKRLGLKFLEIDNEG
                     HSFAVFKEAYEQVCHLMRQGHQIPEIKEKVLCALPQSKDIATTHLAKIRQLLGVA"
     gene            1627..1830
                     /locus_tag="ACF2SP_000865"
     CDS             1627..1830
                     /locus_tag="ACF2SP_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358667.1"
                     /translation="MMKQIPCLKLFTKEELYCLLNACSESLALAYQEIPECDFWHIAM
                     EARLACEALRFEIDSQKKEYSIH"
     gene            1850..2074
                     /gene="csrA"
                     /locus_tag="ACF2SP_000866"
     CDS             1850..2074
                     /gene="csrA"
                     /locus_tag="ACF2SP_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFD2358668.1"
                     /translation="MLILTRRIGETVLINDDIYITVLGVKGNQVRLGFDAPQDVIIHR
                     QEIHQKIKNEQSLFLNKPGKWKEERGVQTH"
     gene            2113..2865
                     /locus_tag="ACF2SP_000867"
     CDS             2113..2865
                     /locus_tag="ACF2SP_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFD2358669.1"
                     /translation="MKPCINQIQKAITTLALTGSFALLVSCAATSTVIEHRNLETDTK
                     LSQTIFLDPVAQSQKTIYITIKNTSQESLSIDKSLKQALEEHGYKVVGNPSKAHYLLQ
                     ANILKVGKMSVSASQSALGGGYGSALAGAATGTALGALSGHSSTMIGAGIGGGIIGLA
                     ADSLVKAVNYTMITDVQISERVGKGAVHEQFNSNLQNGTASGTTQTLSKHSDYQRYRT
                     RVVSNADKVNLNFAAARPALEQGLVKTLAGIF"
     gene            2943..3344
                     /locus_tag="ACF2SP_000868"
     CDS             2943..3344
                     /locus_tag="ACF2SP_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358670.1"
                     /translation="MRSNVIAGLIITALGSPCAVATSSNHYDLERRIFDTSYQLNQIA
                     KENDYDLCSGDVAIAAAYLESAGAQLQHHQKDRAVVSMAYGYNELKEISNVRSYCTHL
                     SPKVKPYLARVIVMKSELENINIPETDQTSD"
     gene            3388..3759
                     /locus_tag="ACF2SP_000869"
     CDS             3388..3759
                     /locus_tag="ACF2SP_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358671.1"
                     /translation="MNVIKTLMLSMTLMIVSGVIHAEPTTLPENNFKINQNLTAASTE
                     NDEQDVEYFKHILSGGALFTLLLSMDKEVPDVAKSVEYVALFKKLDSANHLLTQILVE
                     LKKNNHLLSQSMLDKGVTMNG"
     gene            3866..4177
                     /locus_tag="ACF2SP_000870"
     CDS             3866..4177
                     /locus_tag="ACF2SP_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFD2358672.1"
                     /translation="MNYRTAMNELSIKGYLYARQLLPFLMIGLALLCLMPDSCFAAEN
                     RLSGLKEEVKATFGADSDLPYFLLLAEGLAGAYAYIKTKNIAVLAGVPVLMVFTHWAL
                     K"
     gene            4177..4467
                     /gene="traL"
                     /locus_tag="ACF2SP_000871"
     CDS             4177..4467
                     /gene="traL"
                     /locus_tag="ACF2SP_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFD2358673.1"
                     /translation="MARNYQTFMLSDEPKIAGIPVTTGLPIFLLTGIGLLTGLAYQLF
                     MIGAALSVAMHVKYGGLPLRSLFAIVYWSLPHRLTSLLFRALPDSANRIYIR"
     gene            4472..5032
                     /locus_tag="ACF2SP_000872"
     CDS             4472..5032
                     /locus_tag="ACF2SP_000872"
                     /inference="COORDINATES: protein motif:HMM:NF017152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFD2358674.1"
                     /translation="MDTSFRDNAIAKNRLLFKLTLIWALSSTFAVIVLCALNFYTLLH
                     KQVHWLPVCTGSEFSIGDKGYSPEYLKEMTQKAADLRLTYNPETIDARYTMLSHLIPA
                     KYQESFSKLLDAERKTVHDKNVSSVFYAEKVSVDVTKNQGQIEGQLYRTSHGLQLKPQ
                     HKMYRVQFSHQSGLLNLVSIQEIHHD"
     gene            5025..5759
                     /gene="traK"
                     /locus_tag="ACF2SP_000873"
     CDS             5025..5759
                     /gene="traK"
                     /locus_tag="ACF2SP_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFD2358675.1"
                     /translation="MTNSIYDKAKKTIFLISCLCSGIVGASTAPSIIAFEEGEQFNLT
                     LSSINFNRVFVEGESITKLSYPEHAITVDKSEMDNPESTDSSVYIKPNFQVPITLFLT
                     TDKGHHVSLTLTPDESVGKTLRLVPRAQTKLKFVKLDTPDASEVDEAIAAMKAGETPK
                     EFNEKRVIPSPFYIKKNIKVTLEKQYQGKRFTGYVYRLENTSNHELALTTALFAHKDA
                     QSLSLSDEALPPKKIAYLYGLYSNQG"
     gene            5800..7266
                     /locus_tag="ACF2SP_000874"
     CDS             5800..7266
                     /locus_tag="ACF2SP_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFD2358676.1"
                     /translation="MFKKIKQWLTLKPQNANKLAKKEQLKHAAILLLVGGASYGFYCY
                     SSAEKKKPIHQEEASFESIFESTFNQGSDEALLLQQQQQIDSLKELVAEHHKKQQEAA
                     PVNTENSETKALMQAMKEQLAHLEEENKKMNEQLQVALVSSRQASLVTVRPPTREEME
                     AQQKKKHQAERELLAKSGLETVQFNNRKKRNKEVRTAKNYVWAGTFVEGVLLTGVLGD
                     AGINGSKNMGTALIRLTSGGIMPNNQHSHLEDCVALVSTYGDLSGSSVVMHLETLSCA
                     GNNINFEQKAYGSVFDLDAMQDLRGTSILKTKPLLTYSAAAGMLAGFGDGLKNLNTAQ
                     TINPGAGTITTYGQASTLAQSAAGGALSNPANRISDYVMRIADIYHPLVVARAGRRVS
                     VLFTKGFWIDKEHQVYESGRAINEGRAQNESGVTTTVSRAYELNTHPVNGASLMQSNN
                     QEPMVQTVNPVENPLLNQPGQPTTPLFSTVQNEEPLHD"
     gene            7259..7486
                     /locus_tag="ACF2SP_000875"
     CDS             7259..7486
                     /locus_tag="ACF2SP_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraV"
                     /protein_id="HFD2358677.1"
                     /translation="MIKPLFLLSTLFLVSQLSACSKSILDNEDARCPFADRGGCQSME
                     MVNRMVQEKRYTPDGQFVQQAKSQVLYSGYK"
     gene            7515..10064
                     /gene="traC"
                     /locus_tag="ACF2SP_000876"
     CDS             7515..10064
                     /gene="traC"
                     /locus_tag="ACF2SP_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFD2358678.1"
                     /translation="MFTNAIEWVQDTYELLKETWNDKGLTGNESDFDLKTHQYPSFTE
                     KILPYQYFDEESKLFFNEYNAGLIYRIIPLTGANEHIAEQLDTLLQSKVSHEFTLQLI
                     CVKHNQVGNDIEAFTSQFAKSEFENLSLLGDNLKAFYQKAAIHGFKTNTMLSPRLTHT
                     DCYIVIDKIKKESESDLKACFGQFRVSFEASLSAAKIGFKQADATDFLHLLHFYLDNC
                     PDVITPRPVIYDKTKLLKEQALSHDFDIEFKNNEVVIQGVNESGRIYETAVSVLTIDR
                     LPRQYCLWENINNTSNIFHPDKRISCNHIISVIYLVEEQGRAQGRANRKTRDLDKKSK
                     SDYALNVAGTEEQARVWRTFRDDLSSQKTRSVKMLYNVVLFSRPHEKERDVEAARTVF
                     AFNDIKLALCKRMQCPYFLVSMPFFFTGNLVKDFSLPTMMWPISSWNATQYMPILSDW
                     RGVGKGILLPTMRDQFACIDPFSGVFGSNYNMAVTGTSGGGKSFFIQMLMLNILFNGG
                     DIFIIDVGGSYRKLCEALGGTYLEYSNLAMNPFTHVTDILREIDDIIALFELLTCPTS
                     GATDDDRGTLREAILTAFGATQNQTRIDEVAGVLLSLYEHDRETYPTARILSKNLQRY
                     CSASEHGKAFNEPSQLSPDARMIVVDLKEIEDNQSIRAPVLLYVISQFQRRMFNSDRN
                     KQKMCIIDEAWSFFTGDSIAVNFITKGFRTGRRHKASFVTITQGIEDYFEFSEARAAW
                     ENSALKLVFLQEESPLLEHQKKHETFSDYEMTILKSFPIAKEAGFSQVLLRANGISSF
                     HRLFVDPFTRVLLSSDGDDYQAVINYVAQGLLFLDAVSQVAKEHYGRAYAA"
     gene            10054..10419
                     /locus_tag="ACF2SP_000877"
     CDS             10054..10419
                     /locus_tag="ACF2SP_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI F-type domain-containing protein"
                     /protein_id="HFD2358679.1"
                     /translation="MQLNRFKTMHWVFAGIGGLIGILAGIWVMWPKSLPLLVVDMNRA
                     IEAPSVMLARSKLTHEEQLKIMERFSRTLPEVIKDYGASHQVTLVSASVLSSFKPSHV
                     DVTDELIALTITRIKHEAR"
     gene            10406..11041
                     /gene="traW"
                     /locus_tag="ACF2SP_000878"
     CDS             10406..11041
                     /gene="traW"
                     /locus_tag="ACF2SP_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFD2358680.1"
                     /translation="MKRASKRFFLLILCFFCLLEPVGHAKNIGHYGQTFPVNEEDIRK
                     VIMNKLYALQQSGDLEHVQRDLEQKAARQAMRPNPLALSTTRKPKTFRISPAVTVSHD
                     VWTPDGHLIAKAGTRINPFERVHFLKTLIFFNADDAGQVAWVKKHYTDFKHVKFILTG
                     GDVRVAAELFGRIYFDVNGIISSRLHLQHVPSIVSQEGLDWQVKEIGVNDE"
     gene            11034..12011
                     /gene="traU"
                     /locus_tag="ACF2SP_000879"
     CDS             11034..12011
                     /gene="traU"
                     /locus_tag="ACF2SP_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFD2358681.1"
                     /translation="MNKRFFYSIIVGLFFIGSLQASQCKGHFVNPITDICWDCLFPLT
                     IGSSEVVKSQYPDTKNPGNPICSCPNKLQLLGVTIGYWEPFALVDVTRKPYCMVNLGV
                     QLHIKDQGLGGSQMPDTDGRGAFYYVHWYKYPLMYWLQVLTSIGCMETEDFDVMYLSE
                     LDPTWNDSEFAFVLNPEATLFTTPPVRASCAADATKAFAGTAMDSLFWCMGAQGSTYP
                     LNGFVANQASPISAATLLTERTDFKLHRLGAIKDSVGKDWPALCSTYRSSILPKSRYR
                     YQLVNTLPDAKRCQPFGRSVITWEAGHTYPGDGDNFGFMIWRKRNCCFL"
     gene            12023..12688
                     /gene="trbC"
                     /locus_tag="ACF2SP_000880"
     CDS             12023..12688
                     /gene="trbC"
                     /locus_tag="ACF2SP_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFD2358682.1"
                     /translation="MAHYIKIIILFLTGIGGCFANTNDELRHYQEEGAHQVSTISNKT
                     IETLKRTSSRNQSEHQLLIDTLMQRSTDGLQSNQKPQGAEGAILFVSFSMPDSLLFAL
                     SDEAARFHIPVVINGLVDGDFKQTIETFKRLNSEAKKQHLNFKGISIDPVWFNQFHIT
                     TVPALVVTEPSRSCGSGQPCTNQPFDVVYGNASIKKGLELIAQKGDAGATLAATILEK
                     GHV"
     gene            12681..14492
                     /gene="traN"
                     /locus_tag="ACF2SP_000881"
     CDS             12681..14492
                     /gene="traN"
                     /locus_tag="ACF2SP_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFD2358683.1"
                     /translation="MFRSLLTIACIFGFCTFSAANTTAQDFQKLKEYAKSLGNQPLSA
                     MNEFKPQNTFKDYTETPSQSLHYQGVETEKTDLSTFAANALHNDAGGRTVTEHFGQRQ
                     FEINTKNEAIKNAKLIEEESYAITHGQSNERVKCDEKPQACEIKSHEEICHTSRQLPE
                     QQCFKKRKVTVNTERLSQRADFEVWVHKKWTGLIAVNLITGAMTNSAGGHLTNPVKLN
                     HPCEEMKATVHSILNNGGSAHWVHVVGLPTCQNGGVITLYISDKFSRYYPIQVALTIN
                     AHSKSYVEEEHWDNECATLETNNLCQKTQEQCTDSNPTRVINGLPVTRDCWELNAQYQ
                     CASAAADECKTQREKGCLQASSRCTLMNNNTCSLYEQVYRCDETVCPQPVACVRDLFC
                     ADGDCTEHAATQNDGFGEAMAPMAVAGAAGAEFGKTQATLFSGHPVQCKIWVWDIIDC
                     CSNEGWADKLHIDLCREEDKALGKAKLNYLAHYVGEFCSQKDPIFGTCLEHKRTYCVF
                     DSKMARIIQAEGRLRQLNPNALGDAEHTRCAGLSVNELQSLDMGRIDFLNPVYPFPQG
                     QPTKEAGIVGDVVLNSPDASKTMDEIKRRVQKKAEQK"
     gene            14489..15271
                     /gene="traF"
                     /locus_tag="ACF2SP_000882"
     CDS             14489..15271
                     /gene="traF"
                     /locus_tag="ACF2SP_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFD2358684.1"
                     /translation="MRLFTIILMLLSMPVMADLVSPPAHGFHWYSTENKEHQVKPVQN
                     PKVSKPSLTPYEQLMEVRKATMNKLATALIAPSFDATYEYMKAQQVYAKNNQKFVQYW
                     QQVLLSHPELDHSLNFPTDNTAVAIRNDSMNLLMERVVREGAKRYGLILFYKGNSSIS
                     QKFITHLVPFVNLTHFSMISVTTDGQPIEGLPNPKNIPLHEIQKTMNLQSRYMPALFL
                     VDLKTQHMSPLSYGFVSTTELKERLLDVATHYKRYSYEGFEA"
     gene            15268..15762
                     /gene="trbB"
                     /locus_tag="ACF2SP_000883"
     CDS             15268..15762
                     /gene="trbB"
                     /locus_tag="ACF2SP_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFD2358685.1"
                     /translation="MIVRIIFLLLFSVISPLHANVAVEELNALLLKKQAPKAFAQKES
                     AEAKDLSKNYYFAFIYRSTCPHCKKFSPVLKDFSETFHIKVRAFSLDGESLDGLDTTP
                     LTPELFQTFYVSGGYKPTVPALFLVNRHTLEAYAVLFGEATPYQLAQRVHELKQHIEE
                     KFND"
     gene            15755..17137
                     /locus_tag="ACF2SP_000884"
     CDS             15755..17137
                     /locus_tag="ACF2SP_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFD2358686.1"
                     /translation="MIRALVLAVVASFTCVAGNGIHASASADLNHFFNNLGYSANVTG
                     SHSYESQAAGFASLGSVYARNQVRSIQIAHVDVPGFRSGCGGIDIFAGGFSFIKSEQI
                     VSFMQNILSNGAGYAMNLALETELPEIAHAMQYMQKLANDINGTNFNSCEMGENLTAA
                     LYPKNRAAHQRLCEDIGRNRNVYTDWAEARHKCSTGGEIEKRLEQAKNDSEYKDRALL
                     NTNVVWDALQLNEFIKSDTEISEVYMSISGTLVFDAKGGMQTYPSLATNQDFVKALLY
                     GGKLPSYKCTDSNKPMKCVAINVKGSQEISSKDALVSQVQAILQGVYENIKSGTALTP
                     QQKGLIELTQPSVFQLISASAQQNIGIQSSYELAQSVATDLLAQYLANSLEVIRASLA
                     GRDIGNAHEEKLFKNLQVAQQFVDNFNSESRARFNAALTTNQLVQNNVKQALNALNPI
                     LRQSYTGGAQ"
     gene            17134..20019
                     /locus_tag="ACF2SP_000885"
     CDS             17134..20019
                     /locus_tag="ACF2SP_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFD2358687.1"
                     /translation="MSPLLIYTPQNGIYLKELLDGMATLLTESTFSTACDIIMILSVS
                     MVGYQFVMGKKLESLTRFMLTTFLVMYCVLGIKVPVAIIDMQTAEGAGEALTVDHVPL
                     GVALPASLISGMGYGITTAFSDVFRMPDSLEYNKTGMIFGARTWLAATQTRLSMSPDL
                     AQDLSSYIRQCVFTAKLLASHQLSPQELTQSAHLSKTYFEEPSPIYRVILHNGVNLSC
                     GDAAANLKTRLPIAAKLELERLNHLVTTGALTSRNTDSSSLPGEKTFGDRLEAAHKYY
                     MNITEDAAETLTQNILINATRDAAADAFAFSGADAALMNYTNTDSTQKMHIAEANSFW
                     LAGFRLPYYMTVMWMLTLCIFPLVVLISFFPTLSNAYFLWVQSQIYLWSWPPMFIIFH
                     WFVSMASSTTITLFGQKTGGVTFSNIDSLASMHSNFAYTAGALAASVPVIALYITKGL
                     GQILSTSSQHFGGMAQSLSVSEAQSAAAGNISMASYSGWNMNYENTSANNVSANKHDT
                     NWTNMHGMHTEQLGSGVLKTTTSHGDAVFDVNPGMSRGPVHISDTKALSGSLNQAFEE
                     SRQAAANESQHYQTSLSSFAHSAVQLSKMQGHDMRLGDGVSESESGQYSQALSTMTHI
                     ASDVAKREGISQEDALAHMTAAGLNVHAGVASEKSILGSIGRFAFGATGGADSHAKFD
                     RSSTSSDRYHEGTDSSMSAKEAEDFNQALNYVSQFAQNHHFDNSHSEGASLSNQMGTD
                     LREAQTASQNYDASMSKAARISTAKSYVESNSDQITTDLNQAFPGFVAHRIGEKARDE
                     LYSNPSDLASLKQLQTLANEFISEQREGLIAQYGNASKNARIDSFYQDAANPLSSKES
                     QMRADYHKNSNALEDSGRTMGVGMDASRAKSMQQKINNQVDAAVNKTSSGGGRLKSQY
                     QKAVETTNNDVGEGKKLGQTNVAIPAYFTKWVDHHDKSAPKKELIHE"
     gene            20012..20635
                     /locus_tag="ACF2SP_000886"
     CDS             20012..20635
                     /locus_tag="ACF2SP_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358688.1"
                     /translation="MSNEEMNSQELKEAIALDIETLKTLDVDIMPAKEYYQANRRLFL
                     HVFFKLYGTILLGFLLPLPFYWKVLMVETSTHELIYIFFWLPLMALGLCLFVFLFIYS
                     SLNQYILIDYQLKHKLRTGSLIVKQIRRAGTIAYRIFAGLVLIPSLFLFPSAAFFISF
                     GAFFISGILSSILVEMELNRIGISALFTLIKNYFDKDKNTSAAIPLK"
     gene            20725..22578
                     /gene="traD"
                     /locus_tag="ACF2SP_000887"
     CDS             20725..22578
                     /gene="traD"
                     /locus_tag="ACF2SP_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFD2358689.1"
                     /translation="MSNESNYKHYTRGGQISFHNLRMWFQINKALFHVYLFIWAGLSI
                     VLTCIFAPHGMIRQTVSYHAAHFYHLAGVPHVFSIPVKGGYASQTVEQLLHNHYFSAT
                     SDRLLTLLLQSTLWSFPLSLIVALLVSRYFIYRGKVQTQNQFIRGASLVPSETLKKQI
                     IKQRKASDLHLDGFPLLQGSEVQHLLVHGTVGMGKSQFIMKLMDCLRARGDRVIVYDK
                     GCTFTQAYFQEGHDVLLNPFDARCANWDLWLEAPKDELLENMAESLIPMHGENDPFWV
                     NAARTVFACLASQMREDKERSLSKLLGLLVTGEFSELEPYLSGTAAATLVSNKIEKTA
                     ISIRSVITTYLKSMQSLNGLDESGKPSFSIRDYLLNKDLEGWLFISSNGEQHKSLKPL
                     ISMWIAMASLTLLSLPEDANRRIWFICDELPSLHKLPLLGETIAEVRKFGGCFLLGMQ
                     SFSQLEKVYGRSGAAEIFDLLNTRAFFASPSHEMAQLVSKELGDEEIDDTRENYSYGA
                     NSIRDGISLGKNRITRPLVTYPEIMGLDPLTCYLRLPGQYPITKLELHYQKRSIKAAG
                     FLPRAIPSLPPCDANLKKGSAVNEPTSGRFEVQAPTNIKRIECESDREFIF"
     gene            22593..28430
                     /gene="traI"
                     /locus_tag="ACF2SP_000888"
     CDS             22593..28430
                     /gene="traI"
                     /locus_tag="ACF2SP_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFD2358690.1"
                     /translation="MLSLRVKPIKKPGYYFDQENYYFTNQLVTQWVGLSAARQNLSGE
                     VNVKTLESLVSGELPSGDVIKGLKLTTGEISKRGGYDLTFSAPKSVSYLGLVCEHKEF
                     IDLHLNAVKTVLKLIEKEAAQARKSGKEGMEYEKTGNLCFAAILHDTSRELDPQLHVH
                     ALLMNFTERLDGKWRALASDISRNNGTMEWIMDNQIFLGLVYRSEMALGLKEMGLEIE
                     HTGDAHGLFEIKHFDKALLEQISKRRAQVEEHIKGMHSNSLKAYDRATLDSRKSKEMV
                     SPEELRTRWSAESEALGVNPATYLATLKEKTKESHTPKETMSNNQELDRSVLDAIAHL
                     EEKKLTFTYQEVLQTSLYFSLGEQGFEALMARIDKEIDAQNLIALNTENFSFTTSTLI
                     NKEQELIRKIVNFTPQKKGIERNTDKVCKLTDNESIQKAVTQALFYKEGVVRIKQQSA
                     TSRELLSTLIDYSQDSKKIRILCPSAYSARTMNKDMTQSAPTLWQWILSIGKQDLCET
                     VAGFNYRHRSEHKLPFFNSKKEREVLIVDESQRLTPDEMNILLSIADKRSAKVILLEK
                     SQSLSGFKSDIPDLLNKASIKTFEVDDRKAPATNINLIEEKTIEGRILKTAQMYSNLP
                     LNQRQNTKVLTVSKLEAKEVNEAIRKQLKEHGEISVDEKSINTLTPMSLTLSEKKMAK
                     SYQPDWVLIQNTRTESKKFTVIGVNEKDNQLIVRNHNGVRSQLAAKNITDSTQVYEQT
                     PLSVGIGDQLIATGSLSFEGLKIGNQYEVTAFTRHGIKIKDGKRTIHLITSNEKHFPL
                     SHAYAKTIYSDDFKPVKQTIMTLPAYALKQNTMSVLCESSKEELMIITDDVDKANRFA
                     MKTAAKSSAISLTLNAAKTNHGAQIIDHRATTDLLTSLEQALTILTSEKPQKSDAEKA
                     LNFAIAHLSEREAAFTRSDLLKVALTQAIGKAGLNELNNVLDKVMTNGDLISGGNEIL
                     TTKEAVAFEESIIKNVKAGINTIKPLMSGDEARKQLEPTHLTKGQKEACELITTTTDQ
                     FIMIQGYAGTGKTTMTRSAIDTIRHVQSMTHEEVKLIAVAPTHQAVKEMRALGIEAQT
                     LKSFLIEQEQEVTLSKETLVLLDESSMVSNRDCASLIQKIHDSGARCAMLGDISQHQS
                     IESGKPSKILIQEGSIKVACMDDLVRQQVIEYKKAIETLITGDIDKALAQLANQPLDS
                     ITRTKADSPYYNMTSSIIETGHSTQAYLQQEHTQQESKEPFQEELKEKSPIEMAVGDY
                     LSRTPACRDNTIVIIHENKKREVANGLIRNALMKESTIGLENKEFPRLLSTNYTTAEL
                     YYCETYRDCLKKKEEYFLKKGEHYFKVVSVDEAAKVVVLNDTKGNKCLFVPEKENKDW
                     KIELFQSMPGRVSVGEKIHFKKSDKTLGRFANERVQVTEVNDESFTVKDSSGVAHVLE
                     KKQMKDSHWDYSYTATSYSIQGASSPFVIGVAETKNAQVNHLRSFYIMVTRGSLHAMI
                     YTDDHKKLRKQLRVTPEKTSALESLGLLHTPIKSKTPNEQPKSLKPMHGMPKIKNQEP
                     RYDANMLSQNLSDQAELVIESLLGEPNRALSSKTEYRYGTKGSLSICLSGEKRGVWHN
                     FETKEKGNMLHLIQKTLNLNFKESLEYASKFTGDDLKERIKLASKNPNKIQAIDSDKK
                     RKTSDYGLQLARESKPISGTIAERYLKEIRKIHNVSGENIRFHPHVYTKDTEEVRYRP
                     ALLNIARDKDNKVACVEAVYLDNDTANQAIMKIKPKKSYGLKAGAGVILNEGKGHESV
                     TYIAEGVETGLSIRDAVQNERVIATLGKENFVNIDMALLTDKIVICMDNDGKPIKEDR
                     VIIQTIERLKQHGKTVEIAIPLRQKDFNDVIKSGGIQEVVDTLNKSVSVDKLIGSPNK
                     TDLNQDQIKKCLDTISRQMNLEIPEIKNNSIDKLKTLQREEMEIY"
     gene            28562..>28855
                     /locus_tag="ACF2SP_000889"
     CDS             28562..>28855
                     /locus_tag="ACF2SP_000889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358691.1"
                     /translation="MNNLLQVAYEPSGMQMFFYFISLPFFIILAILSRIHCFYYDVKN
                     GLAFSLLLIWLLYFLFIEYIDQIVHFPNGNELFYYGSLAISLGAFTLIGLTTYF"
BASE COUNT         9047 a         5758 c         6182 g         7868 t
ORIGIN      
        1 taagaattta ccaaaaaaat agtttaaaaa acatcaaaat acaatgcgtt atagcattct
       61 aggtgtgata aagcattata aatttacctt tatggtaaaa atatcattgt aatttttacc
      121 ataaactgat actcttatct aaataaatta tgtaaataaa aggacttgtt gatgaagttg
      181 gatgttgagt tatacattaa tgctaggaaa caaatagaaa aaaagtttca attattgccc
      241 tatggtccta gattaattat ttatgagcta ttaaatcttg caaatcctct tacaggcatt
      301 gtatctgata ttagttatcg tgatctatca aaagccttgg agattaaatc cgctccaggt
      361 cgaatcaaca gtggtattcc atccaagcaa accatcagaa atttcataaa gtctatagaa
      421 catgagtgtg gtgaatactt taaagttata actgaagggc aaaatttaaa atttatgttc
      481 cctgaggctc ctcagatcta taatcaattg attagaaaga aagaagttaa cacagatctt
      541 aattcaccta aatcccttat ggattctgat atagaaggaa agttggaggt acaatctaac
      601 atagaagtta acagagaagc taacacactc aaaagtcctg caaagaatat taaaatattt
      661 aatataacta actcaaacaa acaaactcaa actgcgaata atgatttttg ttgcagcaaa
      721 aaacaaattc atgacgactt ttatccaaac ccaaaaacca tcgaaatggc attttccatg
      781 ggactcacca aagtcaccga tttaaaagaa attcaggcat tcataaaaca taacaaaaaa
      841 cacaacactc aatgggctga ttttaatcca gtattcatta actggttgga aagagattcc
      901 caatataccc aaaacaaaca acaaaaggca caaggacaat taaggagtca tcacaatgag
      961 cgtggtacca atcaaagcac tcttaaccaa acagcacttg aacgagtcag cgaacatcat
     1021 ggcatttcaa tcgactcact ctgggacacc caggacagtg aactcaactc cggtgcattt
     1081 atcgaaggaa cacttgtcca gcctctggat gaagctgacc gcaatatacg ggcaactttt
     1141 tatcagtaag catggagtga aagatacggg agtttggttt gccgcactta aagatttaac
     1201 tccaaaagca ttagacagtg gtgtggaacg attaatggct ttgaataaag gcgacaagtt
     1261 ttgtgaattt ccacccaatt gcctgcagtt tcgagctttg tgtcttggct tttatagtga
     1321 tttgcgactg ccttctgctg cccaggcgca tagggaggta ttaaacagcg cttattcaac
     1381 gaacccaaac tggagtcatg cagtggttaa atttactgct aaaagactgg ggcttaaatt
     1441 tctggaaatt gataatgaag gccattcttt tgcagtattt aaagaagcct atgaacaggt
     1501 atgtcattta atgagacaag gacaccagat cccggaaatt aaagagaagg tcttgtgcgc
     1561 tttgcctcaa tcaaaggaca ttgcaacaac acaccttgcg aaaattcgcc agttattggg
     1621 agttgcatga tgaaacaaat tccatgttta aaactgttta caaaggaaga gctgtactgt
     1681 cttttaaacg cctgctccga gtcattggct ctggcctatc aggaaatccc tgaatgcgat
     1741 ttctggcaca ttgcgatgga ggcacgattg gcatgcgaag cgcttcgatt cgaaattgat
     1801 tcacaaaaaa aagaatattc aattcactaa ccaataaaag gaacacagca tgttaatact
     1861 tacacgtaga ataggcgaaa ccgtactaat caatgatgac atttatatca ccgtgctcgg
     1921 tgttaaaggg aatcaagtcc gcttagggtt cgatgcacca caagatgtaa ttattcatcg
     1981 tcaggaaata catcaaaaaa ttaaaaatga acaatctctg tttttaaata agcctggtaa
     2041 gtggaaggaa gagcgtggcg tacagactca ttaaccaatc aaatcatcat actaacgagt
     2101 taagggaata aaatgaaacc atgcattaac cagatacaaa aggcaattac cactttagct
     2161 ttaactggat cttttgccct gttggtgagc tgtgctgcta catcaacagt aattgaacat
     2221 cgtaacttag aaacagatac aaaactcagc caaacgattt ttcttgatcc tgtggctcaa
     2281 agtcaaaaaa cgatttacat cacaattaag aatacttctc aagagtcatt atccattgat
     2341 aagtcactaa agcaggcatt agaagaacat ggctataaag tggttggaaa tccatccaaa
     2401 gcccattacc tgctacaagc caatatttta aaggttggaa aaatgagcgt gtctgcaagt
     2461 cagtctgcgc taggtggcgg gtatggcagt gcattggcgg gggctgcaac aggaactgca
     2521 ctgggtgcat taagcggtca ttcaagcacc atgattggtg caggtattgg tggaggaatt
     2581 attggtttgg ctgcagattc tctagtcaag gcagtgaatt acacgatgat taccgatgta
     2641 caaatttctg agcgcgttgg aaagggtgcc gttcatgaac agtttaattc caaccttcaa
     2701 aatggcacag catctggaac cacccagaca ttgagcaaac acagtgatta tcaacgctat
     2761 agaacgcggg tggtatccaa tgccgataaa gtcaatctga attttgcagc agcacgaccc
     2821 gcattggaac aaggtctggt aaaaacatta gcgggcatct tttgatgcag cctgattaaa
     2881 gattcataaa aaaaagtgca tgggatttaa aaaaacacta attagttaaa gagggaatag
     2941 taatgagaag caatgtaata gcaggactta tcatcaccgc actgggttca ccctgtgctg
     3001 ttgcaaccag ttcgaatcac tacgatttag aaagacgaat ttttgatacg tcttatcaac
     3061 tcaaccagat tgccaaagaa aacgactacg atttatgttc tggagatgtc gcaattgcag
     3121 cagcctacct ggagtcagca ggagcgcaat tgcagcatca tcaaaaagac agagcagttg
     3181 tttctatggc ttatggatac aatgaattaa aagaaatcag caatgtaaga agctactgta
     3241 cacatttatc accaaaggta aaaccctatc ttgcacgagt cattgtgatg aaaagtgaac
     3301 ttgagaacat caacatacca gaaacagacc aaacatcgga ctaagtttaa aggtttaagt
     3361 tcatagcaaa aataaaagga gcacttaatg aacgttatta aaacactcat gctgtcgatg
     3421 accctcatga ttgtgagtgg agttattcac gcagagccca caacactgcc tgaaaacaac
     3481 tttaagatta atcagaatct gaccgccgct tcaactgaaa atgatgaaca ggatgtcgaa
     3541 tattttaaac acattctttc cggtggagcg ctttttacgc ttttgttatc aatggataag
     3601 gaggttcctg acgtagccaa atcagttgag tatgtggctt tgttcaaaaa gctggattct
     3661 gcaaatcact tactgacaca aattttagtt gaacttaaaa agaacaacca cctattaagt
     3721 caaagcatgc tcgataaagg agtaaccatg aatggataaa ctctctttat aaaaccacaa
     3781 aaacacgatt cattaaataa aaagaataaa aaatgagcac aaattagggc tcaggggatt
     3841 tttttaacca aaaaaggaga atgtgatgaa ctatagaaca gcaatgaacg aattgagcat
     3901 taaaggatat ctgtatgcta ggcaactgct ccctttttta atgattggtt tggcactgtt
     3961 gtgtttgatg cctgactctt gttttgcggc tgagaatcgg ctttctggat taaaagagga
     4021 agtaaaggca acctttggag ctgattcaga tctcccttac tttcttttgt tagcagaagg
     4081 gcttgcaggg gcttatgcct acatcaaaac caaaaatatc gcagtccttg cgggtgttcc
     4141 tgtattgatg gtgtttactc actgggcatt gaaataatgg ctcgcaacta ccaaaccttt
     4201 atgctctctg atgagcccaa aattgcgggt attcctgtta ccacaggcct gcccattttt
     4261 ttactcacgg gaattgggct tttaacagga cttgcctatc agctttttat gataggcgca
     4321 gccctaagcg ttgcgatgca tgtcaagtat ggcggactgc ctctgcggag tctgtttgcc
     4381 atcgtatact ggtcgctgcc tcaccggtta acgtcccttt tatttcgagc tttgcccgat
     4441 tctgccaacc gaatttatat caggtgattt aatggatact tcatttcgtg ataatgcgat
     4501 tgcaaaaaac agactgttat tcaagctgac gctgatttgg gcgctatcga gtacgtttgc
     4561 agttattgtt ctttgtgcct taaattttta taccttactg cataagcagg ttcactggct
     4621 tccagtgtgc actggttctg agtttagcat tggtgataaa ggctattcac ccgagtacct
     4681 gaaagaaatg acgcagaaag cagccgactt acgcctgacc tacaaccctg aaaccattga
     4741 tgcacgctac accatgctgt ctcatctgat tccagcgaaa taccaggaat cgttttctaa
     4801 actattggat gccgagcgaa aaacagtaca tgacaaaaac gtaagctcag tattttacgc
     4861 tgaaaaagta tcggtggatg tcactaaaaa ccaaggtcaa atcgaagggc agttgtatcg
     4921 cacaagccat ggacttcaat taaagccaca acacaaaatg tatcgagttc agttttcaca
     4981 tcaatcaggc cttttaaatc ttgtgtccat tcaggagata caccatgact aactcaattt
     5041 atgataaagc taaaaaaacc atttttctta taagttgttt gtgctcagga attgttggtg
     5101 caagcacagc tccttcgatc atcgcttttg aagaaggaga gcagtttaat ctgaccttat
     5161 cgagtattaa ctttaatcgt gtgtttgttg aaggggaatc gatcactaaa ttaagttacc
     5221 cagagcatgc tattacagta gataaaagtg agatggataa tcccgaaagc acggattctt
     5281 cggtgtatat aaagcccaat tttcaagttc ccattaccct gttcctaact acagataaag
     5341 gacatcacgt atcacttacc ttgacacctg atgaatcggt tggaaaaacc ttaagacttg
     5401 tgcctcgcgc tcaaacaaaa ttgaaatttg tgaaactgga tacccctgat gccagcgaag
     5461 tagatgaggc aatcgctgcc atgaaagcag gggagactcc caaagaattt aatgagaaac
     5521 gggttattcc aagtcctttt tacattaaaa aaaacattaa ggtaactctt gaaaagcaat
     5581 atcagggtaa gcgtttcacg ggctatgtct atcggttaga aaatacatca aatcatgaac
     5641 tagcgcttac aacagcctta tttgcccata aggatgccca atccctgtca ttaagtgatg
     5701 aggcactccc acctaaaaaa atcgcttatt tatacgggtt atacagcaat caggggtaaa
     5761 ccaggagctg cttaaaatga gttggaagga gagtaggtaa tgtttaaaaa aataaaacaa
     5821 tggttgacgc taaagcctca aaacgccaat aagctcgcta aaaaggaaca attgaagcat
     5881 gcagccattc tattactggt gggtggcgca tcctatggat tttattgtta ctcctcagct
     5941 gagaaaaaaa agccaatcca ccaggaagaa gcttcgttcg agagtatttt tgaaagtact
     6001 tttaaccagg gcagtgacga agcactcctt ctgcaacagc agcaacaaat tgattcttta
     6061 aaagagttag tggcagaaca tcataaaaaa cagcaggaag cagcacctgt gaacacagaa
     6121 aattcggaaa caaaagcctt gatgcaggct atgaaagagc agcttgcaca ccttgaagaa
     6181 gaaaataaaa aaatgaacga acaattacag gttgcactgg tttctagccg ccaggcaagc
     6241 ctggtcaccg ttaggccgcc aactcgtgaa gagatggaag cacagcaaaa gaaaaagcat
     6301 caggcagagc gcgaactgct ggcaaaatca ggactggaaa cagtgcaatt taacaaccgg
     6361 aagaaaagaa acaaggaagt acgaaccgct aaaaattacg tttgggcggg aacctttgtt
     6421 gaaggggtgt tgctgactgg ggttttaggt gatgcaggaa taaacggctc aaaaaatatg
     6481 ggaactgctt taatccgcct taccagcgga ggaatcatgc ccaacaacca gcattctcat
     6541 ttggaagact gtgtcgcact ggtatccact tatggtgatt tatcaggaag ttccgtggtg
     6601 atgcatttag aaacactgtc ttgcgcagga aacaacatca attttgaaca aaaggcctac
     6661 ggctctgtat ttgatttgga tgccatgcag gatttacgcg gaacttccat tttaaaaaca
     6721 aaacctctgt taacctattc ggcagcggca ggcatgctgg caggatttgg tgatggttta
     6781 aagaatttaa ataccgctca aacaatcaat ccaggggctg gcaccattac gacctatggg
     6841 caggcatcaa cccttgctca atcggctgct ggtggtgcgc ttagtaatcc tgccaatcgt
     6901 atttcagatt atgtaatgcg cattgccgat atttaccatc cactggttgt ggcacgtgcg
     6961 ggtcgtcgtg tttcagtttt atttactaaa ggcttttgga tagacaaaga acatcaggtg
     7021 tatgagtcag gacgagccat caatgaagga cgtgcccaaa acgagtctgg ggttacaacc
     7081 actgtaagcc gtgcctatga attaaataca cacccagtga atggcgcttc attgatgcag
     7141 tccaataatc aagagcccat ggttcaaacc gttaatcctg ttgagaatcc actattaaat
     7201 caaccaggac aacccactac cccattattt tcaaccgtac aaaatgagga gccacttcat
     7261 gattaaacct ctatttttat tgagtaccct tttcttagtg tcgcagttat ctgcctgctc
     7321 caaatccatc ctggacaacg aggatgccag atgccccttt gctgacaggg gtggttgtca
     7381 aagcatggag atggtgaatc gcatggtaca ggaaaaacgc tacacacccg atggtcaatt
     7441 cgtgcagcaa gcaaaaagcc aggttcttta ttcaggttat aagtaggcgc tgaccagttc
     7501 acaggagaga agccatgttt acaaacgcta tagaatgggt ccaagatact tatgagttgc
     7561 taaaggagac ctggaatgac aaagggctaa cgggcaatga atccgatttt gacctcaaga
     7621 cacatcaata tccttcgttt actgaaaaaa tactgcctta tcaatatttt gatgaggaat
     7681 cgaagctatt ttttaacgaa tacaacgcag gattaattta tcgcattatt cctttaacag
     7741 gtgcgaatga acacatagcg gaacaactgg atacgctgct tcaatctaaa gtgtcgcatg
     7801 agttcacctt gcagttgatt tgcgtcaaac acaatcaagt tgggaatgat attgaagcgt
     7861 ttacttccca gtttgcaaaa agcgaatttg aaaatttaag cctgttagga gataacctta
     7921 aggcctttta tcaaaaagcc gccatccatg gttttaaaac caacacgatg ctttcaccgc
     7981 gattaacgca tacagattgc tatatcgtta ttgataaaat caaaaaagag tccgagagtg
     8041 atttaaaagc ctgttttggc cagtttcgcg tttcgtttga agcatcctta agtgctgcaa
     8101 aaattggatt caagcaagcg gatgcaactg attttttgca tttgcttcac ttttatctgg
     8161 ataattgccc tgatgtcatt actccacgcc ctgtgattta tgataaaacc aaattactca
     8221 aagagcaggc actgtctcat gactttgata ttgaatttaa aaataatgaa gtagtgattc
     8281 aaggagttaa tgaatctggg cgtatttatg aaacagccgt atcagttctg accattgatc
     8341 gcctgcctcg ccagtattgt ttatgggaga acattaataa taccagcaat atttttcatc
     8401 ctgataaaag aatatcctgc aatcacatca tctcggtcat ctatctggtc gaagagcaag
     8461 gacgcgctca gggtagggcg aatcgtaaaa cccgtgattt ggataaaaaa tctaaaagtg
     8521 attacgcatt aaatgtggcg ggaacggagg agcaggcacg ggtatggcgt acctttcgtg
     8581 atgatttatc ctcacaaaag actcgctctg taaagatgct ttataacgtg gttctttttt
     8641 caagacctca tgagaaagag cgtgatgtag aagctgctcg cacagtattt gcgtttaatg
     8701 acattaaact ggcattgtgt aaacgcatgc aatgtcccta ttttttagtc tcgatgccgt
     8761 tttttttcac aggaaatctg gttaaggatt tttctcttcc cacaatgatg tggccaattt
     8821 cttcatggaa tgcaactcaa tacatgccca ttctttctga ctggcgcgga gtaggtaagg
     8881 gtatcttgtt gcctaccatg cgcgatcaat ttgcctgcat cgatcctttc tctggtgtat
     8941 ttggcagcaa ttacaacatg gctgtcaccg gaacctcggg cggtggaaaa agctttttta
     9001 ttcagatgct gatgctcaat atcctgttta atggcggaga tattttcatt attgatgtag
     9061 gtgggagcta tagaaaactg tgtgaggcgc tcggtgggac ttatcttgag tacagcaatt
     9121 tggcgatgaa tccctttacc catgtaacgg acattttacg ggagattgat gatattattg
     9181 ccttgtttga attattaacc tgccctacca gcggtgctac agatgacgac cgaggcactt
     9241 taagagaagc tattttaact gcttttggag caactcagaa tcaaacgcgc attgatgagg
     9301 ttgcaggagt cttattatcg ttgtacgagc acgaccgcga aacctatcct acagcgcgca
     9361 ttttgtctaa gaacctccag cgctactgtt ctgcctcaga gcatggcaaa gcgtttaatg
     9421 aaccctctca gctatcacct gatgcccgta tgattgtagt ggacttgaag gaaattgagg
     9481 acaatcaaag tattcgcgcc ccggttcttc tgtacgttat ttcccagttt caaagacgta
     9541 tgttcaactc agacaggaac aaacaaaaga tgtgcatcat tgatgaagcc tggtcatttt
     9601 ttacaggaga ttcaattgcg gtcaacttta ttaccaaagg ctttcgtact ggtcggcggc
     9661 ataaagcatc atttgtcacg attacccagg gtattgagga ttatttcgaa ttttcagaag
     9721 cgcgcgccgc ctgggaaaat tccgcattaa agcttgtttt tttacaagag gaatcaccgc
     9781 ttttagaaca tcaaaaaaag catgagactt tttcagacta cgagatgact attctcaaat
     9841 cattcccgat agcgaaagaa gcgggttttt ctcaagtctt gctgcgtgct aatggtattt
     9901 cctcctttca tcgcttgttt gttgatccgt ttactcgcgt gcttttatct tccgatggtg
     9961 atgattatca ggcagtcatt aattatgtgg cacagggact gttatttctt gatgccgtat
    10021 cgcaagtggc aaaggagcat tatgggaggg cttatgcagc ttaaccgatt caaaaccatg
    10081 cactgggtct ttgcaggaat tggcggatta attggaatcc tggcgggtat ttgggtgatg
    10141 tggcctaagt ctttacctct tttggtggtt gacatgaatc gtgccattga agcgccttcg
    10201 gtaatgcttg ctcgctccaa actgactcat gaagaacaat taaaaataat ggagcgcttt
    10261 tcacgcaccc tcccagaagt catcaaggat tatggggcgt cacatcaggt aacccttgtg
    10321 agtgcgtccg tattgtcgag ttttaaaccg tcccatgtgg atgtgacgga tgagcttatt
    10381 gccttgacca taacaaggat aaaacatgaa gcgcgctagt aaacgatttt ttctccttat
    10441 tctatgtttt ttctgtctat tagaacctgt gggtcatgca aaaaatatag gtcattatgg
    10501 tcagacattc cctgtcaatg aagaggatat aaggaaagtc attatgaaca aactctatgc
    10561 attgcagcaa agcggtgatt tagagcatgt tcaacgcgat ttagagcaga aagccgcacg
    10621 tcaggcgatg aggccaaacc ctttagcatt gagcacgact cgcaagccta agacgtttcg
    10681 catttcaccg gcagtgactg tatctcatga tgtctggaca cctgatggac atttaattgc
    10741 aaaagcagga actcggatca atccatttga gcgtgttcac ttccttaaaa ccctgatttt
    10801 ttttaatgca gatgatgcag gacaggttgc ctgggttaaa aaacattaca ccgactttaa
    10861 gcacgttaaa tttattttga ccggtgggga tgttcgagtt gctgcagagc tttttggtcg
    10921 aatttatttt gatgtgaacg gcatcatttc atccaggctt caccttcaac acgttccaag
    10981 catagtcagc caggaaggtc ttgattggca ggttaaagaa attggagtga acgatgaata
    11041 aacgattctt ctattcgatt attgtgggct tattcttcat aggttcactg caggcatcac
    11101 aatgtaaggg gcattttgtc aatcctataa ccgacatttg ctgggattgt ttgttcccat
    11161 taaccattgg ctcctccgaa gtggtaaaga gccaatatcc tgatacgaaa aaccctggca
    11221 accctatctg ctcctgccca aacaaattac aacttttagg agttactata ggttattggg
    11281 aaccctttgc gttagtggat gtgacacgaa agccttattg catggtgaat cttggggtgc
    11341 aattgcacat taaagatcag gggctgggtg gttcacaaat gcctgatacc gatgggcggg
    11401 gcgcatttta ttacgtgcat tggtacaaat acccactgat gtattggctg caggtactca
    11461 cttccatcgg ctgtatggag acagaagatt tcgatgtgat gtatttgagt gaacttgatc
    11521 caacatggaa tgactctgag tttgcttttg ttcttaatcc tgaggccact ttatttacca
    11581 caccgccagt gcgtgcgtct tgtgctgcag atgcaaccaa agcattcgca gggacggcca
    11641 tggattcttt attctggtgc atgggcgctc aaggttccac ctatcctctc aatggttttg
    11701 ttgccaatca ggcaagtccc attagtgccg caacacttct gacagaacgc accgacttta
    11761 agcttcatcg ccttggagca attaaagatt ccgttggtaa agattggcct gcactttgta
    11821 gcacctatcg ctcttccatt ttacctaaaa gccggtatcg ctaccaatta gtcaatacat
    11881 tgcctgatgc aaaacgctgc cagccctttg gccgttcagt cattacctgg gaagccggac
    11941 atacctatcc tggggatggg gataattttg gtttcatgat ttggcgcaag agaaattgtt
    12001 gctttttata aggaggcctt tcatggcgca ctacattaaa atcattattc tgtttctaac
    12061 tgggattggc ggatgctttg caaacactaa tgatgagtta aggcattatc aagaggaggg
    12121 ggctcaccag gtatcaacca tttcgaataa gacaattgaa acgttaaagc gcacgtcctc
    12181 acggaatcaa tcagagcacc aattattaat tgatacactc atgcaacgca gcacagacgg
    12241 tttacaaagc aatcaaaaac cgcagggagc tgaaggtgcc attttatttg tatccttttc
    12301 catgcccgac tcacttttat ttgcattgag cgatgaagca gcacggtttc atattcccgt
    12361 ggtcattaat gggttagtgg atggtgattt taaacagacc atagaaacat ttaaacgtct
    12421 taatagcgaa gccaaaaagc agcatttgaa ctttaaggga atttctattg atccagtctg
    12481 gtttaatcaa ttccacatta caacggttcc agcgcttgta gtaacagagc cctccaggtc
    12541 ttgcggttca gggcagccct gcaccaatca accgtttgat gtagtgtatg gcaatgcaag
    12601 cattaaaaaa ggactcgagc tcatcgccca gaaaggagac gcaggagcga cattagcggc
    12661 caccattttg gagaaaggcc atgtttagaa gtttgttgac catcgcctgt atttttggtt
    12721 tttgcacttt ctcggctgcc aatacaacag cccaagactt tcagaaatta aaagaatacg
    12781 caaaatcttt ggggaatcaa ccactgagcg ccatgaatga atttaagccc cagaatacct
    12841 tcaaagacta tacggaaact ccctcgcaat cgcttcatta tcagggggtt gaaactgaaa
    12901 aaaccgattt aagtaccttt gctgccaatg ccttacacaa tgatgccggt ggacgtactg
    12961 tgactgagca ttttggtcag cgccaatttg aaatcaatac caaaaatgaa gcgattaaaa
    13021 acgctaaatt aattgaagaa gaaagttatg ccattactca tggacaatcc aatgagcgag
    13081 tcaaatgtga tgaaaagcca caggcttgtg agattaaatc acatgaggaa atctgccata
    13141 catcaaggca attacccgag cagcagtgtt ttaaaaaacg taaagtaacc gtgaacaccg
    13201 agcgcttaag tcaaagagct gattttgagg tatgggttca taaaaaatgg acggggctta
    13261 ttgcggttaa tcttataacg ggtgccatga ccaatagcgc aggcgggcac ttaaccaatc
    13321 ctgtgaagtt aaatcatccc tgtgaagaaa tgaaagccac ggttcactcc attttaaata
    13381 atggtggttc tgctcattgg gtgcatgttg tgggacttcc aacctgccag aatggaggcg
    13441 taatcacctt gtatatttca gataagttca gtcgctatta cccaattcag gtggcattga
    13501 cgattaatgc ccattccaaa tcctatgtgg aagaagagca ctgggataat gaatgcgcca
    13561 ctcttgagac aaacaaccta tgtcaaaaaa cacaagagca gtgtactgat tccaatccaa
    13621 cccgtgtcat taatggtctg cccgtcaccc gtgattgctg ggagcttaat gcacaatacc
    13681 agtgtgcatc cgctgcagcc gatgaatgta aaacgcaacg ggaaaaagga tgcttacagg
    13741 caagctcgcg ctgcacatta atgaacaata atacctgctc gctctatgag caggtatatc
    13801 gttgcgatga gacagtctgc ccacagcctg tggcctgtgt gcgagatctc ttttgtgccg
    13861 atggagactg cacggaacat gcagcaactc agaatgatgg ttttggagag gccatggcac
    13921 ccatggccgt tgcaggggct gctggcgctg agtttggaaa aacgcaggct accttatttt
    13981 caggccatcc tgtgcagtgc aaaatctggg tttgggacat tattgattgt tgttcgaatg
    14041 aaggctgggc tgacaaactc catatcgatt tgtgtcgtga agaagataag gctttaggca
    14101 aagccaaatt gaattacctg gctcattatg taggcgagtt ttgcagccag aaagatccca
    14161 tttttggcac ctgtctggag cacaaacgca cctattgtgt ctttgacagc aaaatggcgc
    14221 gcattatcca ggccgaagga cgtttaagac aattaaatcc caacgcttta ggagatgcag
    14281 agcatacacg ctgtgcgggg ctcagtgtga atgaattaca aagccttgat atgggacgca
    14341 tcgatttttt aaaccctgtg tatccatttc cccagggaca gccaacaaaa gaagcaggaa
    14401 ttgtaggcga tgtcgtttta aatagccccg acgcttcaaa aaccatggat gaaatcaaac
    14461 ggcgagtcca gaaaaaggcg gaacaaaaat gagattattc actattattt taatgttact
    14521 ttccatgccg gtcatggctg atttagtaag tccacccgct catgggtttc attggtacag
    14581 tacagaaaac aaagagcatc aggtgaaacc tgttcaaaat ccaaaggtat caaagccttc
    14641 gctgactcct tatgagcagc tcatggaagt ccgcaaagcc acaatgaata agctcgcaac
    14701 ggcattaatt gccccatcat ttgatgcaac ctatgagtac atgaaagccc agcaggtgta
    14761 tgccaaaaac aatcagaaat ttgtccagta ctggcaacag gttctgttgt ctcatcctga
    14821 gctggatcat tccttaaatt ttccaaccga caatacggct gtggccattc gcaatgattc
    14881 aatgaatctt ttaatggaga gggtagtcag agaaggggcg aaacgttatg gattaatcct
    14941 tttttacaaa gggaacagtt ccatttcgca aaaattcatc actcatcttg ttccattcgt
    15001 caatttgacc catttttcca tgatttcagt cacgacagat gggcaaccta ttgaaggtct
    15061 gcctaatcct aaaaacatac ctcttcatga aatacaaaaa acaatgaatt tgcagtcacg
    15121 ctacatgcct gcactgtttc ttgttgattt aaaaacacaa cacatgtcgc ccttgtccta
    15181 cggatttgtc tccacaaccg aattaaagga acgtctgctg gatgtggcga ctcattacaa
    15241 acgatacagc tatgaggggt ttgaagcatg attgttcgca tcattttttt actgcttttt
    15301 tctgtaatta gtcctttgca cgccaatgtg gcagttgaag aactcaatgc actgttattg
    15361 aaaaagcaag cccctaaagc ttttgcacaa aaggaatcag ctgaagctaa agatttatcc
    15421 aaaaattatt attttgcatt tatttatcga agcacctgcc cacattgtaa aaaattctcc
    15481 cctgtattaa aggatttttc agaaacattc catattaagg ttcgtgcttt tagcctggat
    15541 ggagaatccc tggatggact cgataccaca ccattaaccc ctgaactttt tcaaacgttc
    15601 tatgtatcag gagggtataa gcctacggtt cccgccttgt ttttagtcaa tcgacatacc
    15661 ctggaagcct acgctgtctt gtttggagaa gcaactcctt atcagctggc acaaagagtt
    15721 catgaattaa agcaacacat tgaggagaaa tttaatgatt agagctcttg tgctagccgt
    15781 tgttgcgtct tttacttgcg tggcaggaaa tggtattcat gcatcagcca gtgccgattt
    15841 aaatcatttt tttaataatc ttggttattc tgcgaatgtc acaggtagcc attcctatga
    15901 atcacaggca gcaggatttg catcattagg ctcagtctat gccagaaatc aggtgcgctc
    15961 cattcagata gctcacgttg atgtacctgg ttttcgctca ggatgtggcg gaattgatat
    16021 ttttgcaggt ggtttttcct ttattaaatc agagcagata gtttcattta tgcagaacat
    16081 tttaagtaac ggggcaggct atgcaatgaa cctggcactc gagaccgagc tgcctgaaat
    16141 tgctcatgcc atgcagtaca tgcaaaagct ggcaaatgac attaatggaa ctaattttaa
    16201 ttcgtgtgag atgggagaaa atctgactgc agcgctttat cccaaaaata gagcggcaca
    16261 tcagcgtttg tgtgaggaca tcgggcgtaa ccgtaatgta tacaccgatt gggcagaagc
    16321 ccgacataaa tgttcgacag gaggggagat tgaaaagcga ttagaacagg caaaaaacga
    16381 ttcagaatat aaagacaggg ctcttttgaa taccaacgta gtttgggatg cattacaact
    16441 gaatgaattc atcaaatcag atacagaaat ttcagaagtc tatatgtcaa tttcaggaac
    16501 gctggtattt gatgccaaag gagggatgca aacgtatccc tctttagcga ctaatcagga
    16561 ttttgtaaaa gccttgcttt atggaggcaa attgcccagc tataaatgta ctgattcgaa
    16621 taagccgatg aaatgtgttg ccatcaatgt gaaaggaagc caggagatta gcagtaaaga
    16681 cgctctggta tctcaagtac aggccatcct gcagggagtt tatgagaata tcaaatcagg
    16741 gacagcctta acgccccaac aaaaagggtt aattgaactc acccagccgt ccgtatttca
    16801 gcttatttca gccagcgcac agcagaatat tggcatacaa agcagttatg agcttgccca
    16861 gagtgtagca acggatttgc ttgcccaata tcttgcaaac tcacttgaag tgattcgagc
    16921 atcccttgca gggcgtgata taggaaatgc ccatgaagaa aaactattta aaaatctgca
    16981 ggttgcacaa caatttgtag ataattttaa tagcgaatca agagcacgtt ttaatgcggc
    17041 actgaccacc aatcaattgg ttcaaaacaa cgtgaagcag gcactgaatg cccttaatcc
    17101 catcttaaga caatcctata ccggaggcgc acaatgagcc cattattaat ctatacccca
    17161 caaaatggga tttatttaaa agagctgctt gatggtatgg ccactttgct tactgaatct
    17221 acctttagta cggcgtgtga cattatcatg attctatccg ttagtatggt cggttatcag
    17281 ttcgtgatgg ggaaaaagct cgaatcatta actcgcttta tgctgaccac ctttttagtc
    17341 atgtattgcg tgctgggcat taaagtgcct gtggctatta ttgacatgca gacagccgaa
    17401 ggagcaggag aagccttaac ggttgatcat gtacctctag gcgttgcttt acctgcctca
    17461 ttgattagtg gcatggggta tggaattacg actgccttta gtgatgtatt tcgcatgcca
    17521 gatagccttg agtacaacaa aacaggaatg atttttggtg caaggacttg gcttgcagca
    17581 actcagacaa ggctctccat gtcgcctgat ttggcacaag acctatcgtc ctatatccgc
    17641 caatgcgtgt ttacggcaaa acttttggca agccatcaat taagccctca agagctgacc
    17701 caaagtgctc atctttctaa aacctatttt gaggagccat cacccattta tcgggtgatt
    17761 ctgcacaatg gagtcaattt aagttgtggt gatgcggcag ccaatttgaa gactcgactg
    17821 cccattgcag cgaaattgga gttggagcgt cttaatcatc tggtaaccac aggcgcttta
    17881 acatcacgca acacagacag cagttcgttg cctggcgaaa agacttttgg tgacaggttg
    17941 gaggccgctc ataaatatta catgaatatt accgaagatg ctgccgaaac actcacccaa
    18001 aatatcttaa ttaatgcgac ccgagatgcc gcagccgatg cttttgcttt ttcaggagct
    18061 gatgctgcac tcatgaatta caccaatacg gacagcaccc aaaaaatgca catcgctgaa
    18121 gccaatagct tttggcttgc aggatttcgc ctgccttact atatgaccgt gatgtggatg
    18181 ttgacgctgt gtatctttcc attagtcgtt ttgatttcat tttttccgac cctgagtaac
    18241 gcttatttcc tgtgggtgca atctcaaatc tacctttggt catggccgcc gatgtttatt
    18301 atttttcatt ggtttgtttc aatggcctca agtactacca tcactttgtt tggtcaaaaa
    18361 acaggaggag tgaccttctc taatatcgat tcattagcca gcatgcacag taattttgcc
    18421 tatacggcag gtgcattggc tgcaagtgtt ccagttattg ccctgtacat cacgaaagga
    18481 ttaggacaga ttttaagtac atcctcccag cattttggtg gtatggcaca atctttgtca
    18541 gtcagtgaag cgcaatctgc agctgcaggt aacatctcaa tggcaagtta cagcggttgg
    18601 aatatgaact atgaaaatac cagcgccaat aacgtttcgg ctaacaagca cgataccaat
    18661 tggactaaca tgcatgggat gcacacggaa caattgggaa gtggtgtgct taagactacc
    18721 acaagccatg gggatgctgt ttttgacgta aatcctggga tgtcacgagg acccgtacac
    18781 atttcagata caaaggcctt aagcggctct ttaaatcagg cttttgaaga atccagacag
    18841 gctgcagcca atgaaagtca gcattatcaa acgtccttgt ccagttttgc gcacagtgcc
    18901 gtgcagttat ctaaaatgca gggtcatgat atgcgtttgg gcgatggagt gtctgaaagt
    18961 gaatccggtc aatacagcca ggcattatcg accatgacgc atattgcctc ggatgtagct
    19021 aaacgcgaag ggataagcca agaggatgcg ctggctcata tgaccgctgc tggattaaat
    19081 gtccacgccg gagttgcaag tgaaaaaagc atactgggaa gcattgggcg gtttgctttt
    19141 ggagcaacag gaggtgctga tagccatgct aagttcgatc ggtcatcaac aagtagtgat
    19201 cgttaccatg aagggacgga cagcagcatg tcagcgaagg aagcagaaga ctttaatcag
    19261 gcgcttaatt acgtgagtca gtttgcacaa aaccatcatt ttgataacag ccattcggaa
    19321 ggggcaagcc tttcgaatca aatgggtacc gatttaaggg aggcgcaaac ggcaagccaa
    19381 aactacgatg cctcgatgtc taaagccgct cgcattagca cggccaagag ttatgtggaa
    19441 tcaaactccg atcagattac caccgatctt aatcaggcat ttcctggttt tgtggcacac
    19501 cgcattggag agaaggcacg agatgaattg tattccaatc cttcagatct ggcctcatta
    19561 aaacaattac aaacgctagc taatgagttt atcagtgaac aacgagaagg tttaatcgca
    19621 caatacggca atgcttctaa aaatgctcgg attgactcct tttatcagga tgcagcaaat
    19681 ccattaagtt caaaagaatc acaaatgaga gctgactacc acaaaaacag taatgcattg
    19741 gaggattctg gaaggactat gggagttggc atggatgcat caagagccaa aagtatgcag
    19801 caaaaaatta ataatcaggt agatgcggcg gtaaataaaa caagcagtgg aggagggcgt
    19861 ttaaagagtc aataccagaa agcggttgaa acaacaaaca acgatgtagg cgaaggtaaa
    19921 aaactggggc aaaccaacgt ggccataccc gcctatttta ctaaatgggt tgatcatcat
    19981 gataaatcag ccccaaagaa ggagcttatc catgagtaat gaagaaatga attcacaaga
    20041 attaaaagag gccatcgcct tggatataga aacattaaaa acattggacg tggatattat
    20101 gccggccaag gagtattacc aggcgaacag gcgtttgttt ttgcatgttt tttttaaact
    20161 atatggaaca atacttctag gatttttact tccacttcct ttttattgga aggtgttgat
    20221 ggtggagact tcaacacacg agctcatcta catttttttc tggctgccat taatggcttt
    20281 aggactatgc ctttttgttt ttctattcat ttacagctca ttaaatcaat acattttgat
    20341 tgactatcaa ttgaaacaca aactaagaac aggctcattg attgtaaaac aaataaggcg
    20401 ggctggaact atagcttatc gaatatttgc agggcttgtt ttgattcctt cactgttctt
    20461 gtttcccagt gcagccttct tcatatcctt tggcgctttt ttcatcagcg ggattttatc
    20521 gagcatcctt gttgaaatgg agctcaacag gataggaatc agtgctctgt ttactctcat
    20581 aaagaattat tttgataagg acaaaaatac cagtgcggca atacccttaa aataaacaaa
    20641 acacatcaaa aagaataatt tttaaccaca aatacaagca gtggaagccg ctgctttgcc
    20701 caaaataagc aggaggaaga taaaatgagc aatgaatcca attacaaaca ttatacccgg
    20761 ggaggtcaga tctcattcca taatctgcgg atgtggtttc agattaacaa agcactcttt
    20821 catgtttact tattcatttg ggcagggtta agcattgtat tgacctgtat ctttgcgcca
    20881 catggaatga taagacagac ggtgtcctat catgcggctc atttttatca cctggcaggg
    20941 gtgccacatg tattttctat ccctgttaaa ggggggtatg catcacaaac tgtcgagcag
    21001 cttctccaca atcactattt ttctgcaacc tcagatcgtt tactgaccct gctgttacag
    21061 tcaactctat ggagttttcc cttatcgttg attgtggcac ttttggtgag ccgatatttc
    21121 atttatcgag gaaaggtaca aacacaaaac caatttatac gaggggcgag tttagtccca
    21181 tcagaaacac ttaaaaaaca gataataaag caaagaaaag catctgattt acatcttgat
    21241 ggattccctc ttttgcaagg ctcagaggta cagcatttat tagtacatgg tacggtcggt
    21301 atgggaaaaa gtcagttcat catgaaattg atggattgtt tacgtgcgcg tggcgaccga
    21361 gtcattgttt atgataaggg gtgcacgttt actcaggcgt attttcagga aggtcatgat
    21421 gtcttactca atccttttga tgcacgctgc gctaattggg atttatggct ggaagcccca
    21481 aaagatgaat tactggaaaa tatggcagaa agtcttatcc ctatgcatgg agaaaacgat
    21541 ccgttttggg ttaatgcagc acgtaccgtg tttgcctgtc ttgcaagtca aatgcgtgag
    21601 gataaagaac gttccttatc caagctgtta gggttacttg taactggcga gtttagcgag
    21661 ttggaacctt atctcagcgg tacagccgca gcgactttgg tcagtaataa aatagaaaaa
    21721 acagccatct ccattcgctc tgtcattaca acctacctta aatcaatgca gtcgttaaat
    21781 gggcttgatg agtctgggaa gccttccttt tccattcgtg attatctgtt aaataaagat
    21841 ttggagggct ggctttttat atcaagcaat ggggaacaac ataaatcttt aaagccgttg
    21901 atttcgatgt ggattgccat ggcatcactg acactcttaa gtttgccaga agacgcgaac
    21961 aggcgaattt ggtttatttg tgatgaatta ccaagcctgc acaaattacc cttattgggt
    22021 gaaaccattg ctgaagtcag gaaatttggc ggctgttttc ttctaggaat gcagagcttt
    22081 agtcaattgg agaaggtata tgggcgtagt ggggcagctg aaatttttga tttgcttaat
    22141 acccgagcct tttttgcaag tccaagccat gaaatggcgc aacttgtcag taaagagctt
    22201 ggggatgaag aaattgatga cacccgagaa aattactctt atggagccaa ctccattcgt
    22261 gacggtattt cattaggaaa aaaccgtata acacgcccct tagtaaccta tcctgaaatt
    22321 atgggacttg atccgcttac ctgttatctg cgattgcctg gtcaataccc tattacaaag
    22381 ctggaacttc attatcaaaa aagaagtata aaggccgcag gttttttacc tcgtgccata
    22441 ccgtcccttc ctccgtgtga tgcaaatctc aaaaaaggtt ctgctgttaa tgagcccacc
    22501 agcggtcgtt ttgaggtgca agcgccaacc aacatcaaac gtatcgaatg cgagtccgat
    22561 agggaattca ttttttgata ggggaataaa tcatgttaag tctgcgagtc aaaccaatta
    22621 aaaagcctgg ttattatttt gatcaggaaa attattattt tacgaaccaa ttggttaccc
    22681 aatgggttgg attaagtgct gcacgtcaaa atctttctgg agaagttaac gtaaaaaccc
    22741 ttgagtctct tgtcagtggc gaattaccaa gcggtgatgt gattaaaggc ttgaaattaa
    22801 ctactggtga aataagcaag cgaggcggct atgatttaac tttttctgcc ccaaaatccg
    22861 tatcctatct tgggctggtc tgtgaacata aagaatttat tgatttgcac ctcaatgcgg
    22921 taaagacagt cttaaaactg attgaaaaag aggcggcaca agcgcgcaaa tcaggaaaag
    22981 aaggcatgga atatgaaaaa acggggaatc tttgttttgc tgccattctc catgatacct
    23041 ccagagagct tgatcctcag cttcatgtgc atgcattgtt gatgaatttc accgagcggc
    23101 tggatggcaa atggcgtgct ttggcttctg atatcagtcg caataatggc accatggagt
    23161 ggattatgga taatcaaatc tttttgggtc ttgtgtaccg ctctgaaatg gctctcggtt
    23221 taaaagaaat ggggctcgaa atagagcata cgggtgatgc tcatggtcta tttgaaatca
    23281 agcattttga taaggcattg ttggagcaaa tctctaagcg ccgtgcccag gttgaagaac
    23341 atattaaagg gatgcactct aattcattaa aagcgtatga cagggcgact ctggattcaa
    23401 ggaaatcaaa ggaaatggtt tctccagagg aattacgcac acgatggagc gctgaaagtg
    23461 aagcgttggg cgttaatcca gccacctatc ttgccacctt aaaagaaaaa actaaagaat
    23521 cgcatacacc aaaagaaacc atgtccaata atcaagaact cgatagaagt gttcttgacg
    23581 caattgcgca tttagaagaa aaaaaactaa cctttaccta tcaggaagta ttacagacga
    23641 gcctttattt ttccttaggt gagcagggct ttgaagcact gatggcacgg attgataaag
    23701 aaattgatgc acaaaatctg attgctctta atacagaaaa cttctctttt acaacgagta
    23761 cgctcattaa caaggaacaa gaactcatta gaaaaatcgt gaacttcaca ccccaaaaaa
    23821 aaggcataga acgcaatacg gataaggttt gcaaactcac tgataatgag tccattcaaa
    23881 aagcagtaac tcaagcctta ttttataaag aaggggttgt gcgcatcaaa cagcaaagtg
    23941 ctacctcgcg tgagctctta agtactttaa ttgattattc acaggattca aaaaaaattc
    24001 gtattctttg tccttctgct tattctgcgc ggacgatgaa taaggacatg acccaatcag
    24061 cgcctaccct gtggcaatgg attttatcta ttggaaaaca ggatttgtgt gaaacagttg
    24121 cgggctttaa ttatcgccat cgttcagagc ataaattacc tttttttaac agtaaaaagg
    24181 agcgcgaggt gctgattgtg gatgaatcac agcgtcttac tcctgacgag atgaatattt
    24241 tattatctat tgccgataag cgtagtgcca aagtcatttt gctggaaaaa tcacaatccc
    24301 tttctggttt taaatccgat attcccgact tgctgaataa agccagcata aaaacatttg
    24361 aggttgatga caggaaagcg cctgccacca acatcaatct catagaagaa aaaacgattg
    24421 aagggcgtat tctaaaaaca gcacaaatgt acagtaactt accgttgaac cagaggcaga
    24481 atacaaaagt attaactgtc tcaaagctcg aagcgaaaga ggtcaatgaa gcaatccgta
    24541 agcagttaaa agagcacggg gagatttcag tagatgaaaa gagcatcaat acgttaaccc
    24601 ctatgtcctt gaccttaagt gaaaaaaaaa tggccaaaag ttatcagccc gattgggtat
    24661 taatccagaa cacccgtaca gaatcaaaaa aattcacggt aatcggtgtc aatgaaaaag
    24721 acaatcaatt gattgttcgg aatcataacg gggtaagatc acaacttgct gctaaaaata
    24781 ttacagattc cacgcaggtt tatgaacaaa caccattatc agtaggtata ggagatcaat
    24841 taattgctac gggcagttta tcctttgaag gattaaaaat cggtaaccaa tatgaggtca
    24901 ctgcatttac ccgccatgga ataaaaataa aagatggaaa aagaactatt catcttatca
    24961 cgagtaacga aaagcatttc ccattgagcc atgcctatgc caaaaccatt tattctgatg
    25021 attttaaacc cgtgaaacaa accattatga cactaccggc ctatgcgctc aaacaaaata
    25081 ccatgtcggt attatgcgaa tcgagtaaag aagaattaat gattattacg gatgatgtgg
    25141 acaaggctaa tcggtttgca atgaagactg cagcaaaatc ctcggcgata tcgctgactt
    25201 tgaatgctgc caaaaccaat catggtgcac aaattattga ccatagagcc acgactgatc
    25261 tgctcacttc actggagcag gccttaacta ttttaacctc ggaaaaacct caaaaaagtg
    25321 atgcagaaaa agcgctgaac tttgcaatag cccatctttc agaacgagaa gcagccttta
    25381 ctcggtcaga tttgcttaag gttgcgctca ctcaggctat aggaaaagca ggacttaacg
    25441 aattaaataa cgtcctggat aaggtcatga ctaatgggga tttaatatca ggggggaacg
    25501 agattttgac caccaaagaa gcggtggcat ttgaagagtc gattatcaag aatgttaaag
    25561 caggtattaa caccataaag ccgctaatga gtggtgatga agcacgaaaa caattagagc
    25621 ccacacacct taccaaaggc caaaaagaag catgtgagtt aatcacgact acaactgatc
    25681 agtttattat gattcagggt tatgcaggaa caggaaaaac aaccatgact cgaagtgcga
    25741 ttgacaccat caggcatgtc caatctatga ctcatgaaga ggtgaaactg attgcggtcg
    25801 cgcccacgca ccaggctgta aaagaaatga gggcgctggg aattgaagcg caaacattaa
    25861 agagttttct tattgaacaa gaacaggaag tgacattaag caaagaaaca ctggtgctgc
    25921 ttgatgaatc ttctatggtc tctaatcgag attgcgcaag cctcatccaa aaaatccatg
    25981 attcgggcgc tcgttgcgca atgcttggcg atattagcca gcatcaaagc attgaaagcg
    26041 gtaagccaag caagatattg attcaggaag gaagcatcaa ggtggcctgt atggatgatt
    26101 tggtacgaca acaagtcatc gaatacaaaa aggcaatcga aacattaatc acgggggata
    26161 ttgataaggc gttggctcag ttagcgaatc aacccttaga ctcaatcacc cgaactaaag
    26221 ccgatagtcc ttactacaac atgacctctt caattattga aacaggccat tcgacccaag
    26281 cgtacctgca acaagaacac actcaacaag aatcgaaaga acccttccag gaagaattaa
    26341 aggaaaaaag cccgatagaa atggctgtag gagattattt atcacggaca ccagcatgcc
    26401 gtgataacac cattgttatc attcatgaaa ataaaaaaag ggaagttgca aatggtctga
    26461 ttagaaatgc actgatgaaa gagtctacca taggccttga aaacaaagag ttcccgcggc
    26521 tactaagcac gaattacacc actgcagagc tttattattg tgaaacgtat cgggattgct
    26581 taaaaaagaa agaagagtat tttttaaaga aaggagagca ctatttcaaa gtggtttcgg
    26641 tagatgaggc agcgaaagta gtggtattaa atgatacgaa aggaaataaa tgcctctttg
    26701 ttcctgaaaa agaaaataaa gactggaaga ttgaattgtt tcaatccatg ccaggaagag
    26761 tttcggtggg tgaaaaaatt cattttaaaa aatctgataa gactttggga cgtttcgcta
    26821 atgaaagggt gcaggtgacg gaggtaaacg atgagtcatt taccgtaaaa gacagtagtg
    26881 gcgtggcgca cgttctggaa aaaaaacaga tgaaggattc ccattgggat tatagttata
    26941 ctgctaccag ttattcaatt caaggggctt catccccatt tgttattgga gttgctgaaa
    27001 ctaaaaatgc tcaagtaaac catctgcgtt cattttatat catggtaacg cgaggttcct
    27061 tgcatgccat gatttatacg gacgatcata aaaaactacg aaaacaactt cgtgttactc
    27121 ctgaaaaaac ttctgcattg gaatcgcttg gcctcctgca taccccaata aagtccaaaa
    27181 ctcctaatga gcaaccaaaa tcactcaagc caatgcatgg tatgcccaaa ataaagaacc
    27241 aggaacctcg ctatgatgcg aatatgctgt cacaaaattt atccgaccag gctgagctcg
    27301 tgattgaatc actacttgga gagcccaata gagcgctttc gtcaaaaaca gaatatcgat
    27361 atggtactaa aggcagctta agcatctgtt taagtggaga aaaaagagga gtttggcata
    27421 actttgaaac aaaggaaaaa ggaaacatgc ttcatttgat tcaaaaaaca ttaaatctaa
    27481 attttaaaga aagtcttgaa tatgcttcca aattcacagg tgatgactta aaagaacgta
    27541 ttaaattagc aagcaaaaac cctaataaga ttcaagcaat cgattctgat aagaaaagaa
    27601 aaacatcaga ttatggactg caattagcac gagaatcgaa acccatatca ggcacaatag
    27661 cagagcggta tttaaaagaa atcaggaaaa ttcataatgt gtctggagaa aatattcgct
    27721 ttcatcctca tgtctatact aaagataccg aagaagttcg ctacaggcca gcactattga
    27781 atattgcgcg ggacaaagac aataaagtag cctgcgttga agcagtatac ctggataatg
    27841 acacagcaaa tcaagcaata atgaagataa agcctaagaa atcttatgga ttaaaagcag
    27901 gcgctggagt catattaaat gaggggaaag gacatgaaag tgtcacctat atcgcagaag
    27961 gtgtagaaac aggactaagc atcagagatg cagttcaaaa tgaacgagtt atagcgacac
    28021 tagggaaaga aaattttgta aatatcgata tggctttgct caccgataaa atagtcattt
    28081 gcatggataa tgatggaaaa cccattaaag aggacagggt aattattcaa accattgaac
    28141 gattgaaaca gcatggaaaa accgtagaaa ttgccattcc tctgcgtcaa aaagacttta
    28201 atgatgtgat taaaagcggg ggtatacaag aagtagttga tacattaaat aaatcagtta
    28261 gtgtcgataa actgattgga agccccaata aaacagattt gaatcaagat caaattaaga
    28321 agtgccttga taccatttca cgacagatga accttgaaat tcctgaaatt aaaaacaatt
    28381 caattgataa attgaaaacc cttcagcgag aagaaatgga aatatattaa aatatcttta
    28441 attgattgag cataggaatt ttatatgaaa aaaatgtgtg gaataatctc tctaatattg
    28501 ataaatggag gtatcattct accttattta tgtctatgta ttagtagctt gttccaccaa
    28561 aatgaacaac ctattacagg ttgcttatga gccatctgga atgcaaatgt ttttttattt
    28621 tatatcgctc ccgtttttta taattttagc aatattaagt cgtattcatt gtttttatta
    28681 tgatgtaaaa aatggattag cgttttcgtt gttattgatt tggttattgt attttttatt
    28741 tatcgaatat attgaccaaa tagttcattt tcccaatgga aatgaactat tttattatgg
    28801 tagtttagct atttcattag gggcatttac tttaattgga ttaactacct atttc
//
LOCUS       DAXLDT010000025        28848 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXLDT010000025 DAXLDT010000000
VERSION     DAXLDT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 28848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28848
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.025"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            40..537
                     /locus_tag="ACF2SP_000890"
     CDS             40..537
                     /locus_tag="ACF2SP_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358692.1"
                     /translation="MKQLILHPTDISQWHALVNEAQAATRLILNENTESYLVFLLMRF
                     SQGTKLIESVVALDFLESMRKPRQLQVELLRDVGDKSLLFCGLFPGIAKKRHVSLSYF
                     SDMGQAAYLTVGELEENQTANLYMQLSEQFITLQQVLQAMRGEYIQFSQSSCGVLAEP
                     DLTMQ"
     gene            complement(551..1396)
                     /gene="hslO"
                     /locus_tag="ACF2SP_000891"
     CDS             complement(551..1396)
                     /gene="hslO"
                     /locus_tag="ACF2SP_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFD2358693.1"
                     /translation="MKEADTLQRFIFEHANIRGEIVHIEKTFQNIMNQRDYPPMVKNL
                     LGEALVSCLLLASSIKFEGNLSLQFQGDERLSLLLVQCDHNLNIRGFAKCAEGLEIAD
                     YATAFLQGRMVITLSQDNQTQAYQSLIPIQSTSMSENLMTYFAQSEQIATRVWLAVNE
                     DMAAGMLLQLMPGQDTIQREQFWEYAVQLGQTVTEDELLTIDNQTLLYRLYHETELRI
                     FESRSTRFQCRCNQDKMKQVITVLGEEEATDLIKEKGKIEITCDFCNQKYTFDSIDVT
                     LLFRK"
     gene            1604..1753
                     /locus_tag="ACF2SP_000892"
     CDS             1604..1753
                     /locus_tag="ACF2SP_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358694.1"
                     /translation="MKNNSLLLPLFKTIDKLLFGIEEIKEIEKTPEYILCDNEDEFDV
                     ERSIN"
     gene            1812..3809
                     /locus_tag="ACF2SP_000893"
     CDS             1812..3809
                     /locus_tag="ACF2SP_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFD2358695.1"
                     /translation="MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGY
                     NANSICAVTFTNKAANEMRARVSAFLPAVSRRGLKVATFHTLGLSIIKRDVSRCDLKP
                     GFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
                     CDEALTIYPRYQEALKAYNAVDFDDLIRLPVNLLNDHADVLEYWQNKIRHLLVDEYQD
                     SNTSQYLLVKKLAGVRAHFTVVGDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQN
                     YRSTSRILHAANHLIAHNQHLFEKKLWSDLGHGELLRVVSCKDEQDEAEHVIADLISH
                     KLRNRTNYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKL
                     LCNEADDAAFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLALSEFVADKQR
                     MIFHDFKLWMEDIKKRISSDSVLEHLRQMVEDIGYEAYIYEQCDSPAKAQKKMDNVWE
                     LLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGLEFPFVY
                     LVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQRGLCFTLAKQRRRAGELQDCVPS
                     RFLDELPQESLEWFGKSGERCEEQSKKLAKSHLEGLKNLLS"
     gene            complement(3893..5875)
                     /gene="iroT"
                     /locus_tag="ACF2SP_000894"
                     /gene_synonym="mavN"
     CDS             complement(3893..5875)
                     /gene="iroT"
                     /locus_tag="ACF2SP_000894"
                     /gene_synonym="mavN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector ferrous iron transporter
                     IroT/MavN"
                     /protein_id="HFD2358696.1"
                     /translation="MNIKNIPIKPQKIPYYLFLLLLTVGASLILGFLSFGGMYALWPV
                     LPLAFAGFALSVAYEGEIYLQNIKGALNKLFKFNYLKNHFAKEYLLNHFPNTNEENCP
                     QFFKDYKRQLKLLAEFGHKELNQDSKKRKRQIEKTLSDMEKWFALQLFSDKNNPSPNT
                     SKYARELLDWLALNDQDEWIKRWEKRHFQFHIVKGFSLVAGLFMGLGSTYLIVEAFTI
                     IPFFAAIPFTFWPIIILPMAIVAGAAYGMLTYNAVTDMINNNTVVKWYNKIRDDLSQG
                     LTVRNVFIASTAVFLVGLAVALTICTAGTWWTIATNARPLFDWMKKMPSFIMGIINPI
                     ITGASAIVFNIQNTAESLDMVDEATRSNKNVFQKIYEAISNGYQHLRETENWLQIVNP
                     FRILLKLTITPLRILLFLGHLISVAVTSDRMPGVPQILSALVAIISEGFEDAHYFIGH
                     THEDEQDEEHGHQDHHQFEKLLKERLDPDSDQDHNMDIPTWILKTIASPIYGLAALWD
                     CSASKLNPPQENHSKNLPSGKKPHVLSLGEAWNKQWGVAKEFNVELSTHAKRPSQEWQ
                     VEHAVAQIDKFQRKHLKDIVIGRELADKKVIALNHLKEKIRHPEKGETLSDTLEQAKK
                     QPDYNQHRLFNQKGEKTRTQVFIEELPERINLSSGK"
     gene            6056..7261
                     /gene="lapA"
                     /locus_tag="ACF2SP_000895"
     CDS             6056..7261
                     /gene="lapA"
                     /locus_tag="ACF2SP_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase LapA"
                     /protein_id="HFD2358697.1"
                     /translation="MRFNQWMTCITSGLILASNSSFATTSPVHEQLQVPQCLAAKITV
                     PHKILAENKEFKIIDVLSSDVETLTILADQVSCGHFVNVSHKLTGTLAANQQQSAQKL
                     LQKKLVKPFGVSKLHKDVYEIKHEEEVNAALKEIVSDNIWQTLTHMTSYYNRSATKDT
                     GVETANWLKSKFEQMAVEYGRTDTSTFFVKTGWYKQPSLVTVIGKDIKAPAIVIGAHM
                     DTLDGRMPGAGDDGSGSSSIMEAARVILSSKTTFKRPIYFIWYAAEERGLVGSQHVVQ
                     HFQEQSIPVKAVVQFDMTGYRNDANDPTMWVFTDYTDRDLSNYLAKLIDHYIHVPVDY
                     SRCGYGCSDHASWNEEDIPAAFPCETSFADHNPYIHTSSDKMDLLNLEHMTNFSKLAV
                     AFAIELASE"
     gene            complement(7342..8202)
                     /gene="vipE"
                     /locus_tag="ACF2SP_000896"
     CDS             complement(7342..8202)
                     /gene="vipE"
                     /locus_tag="ACF2SP_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipE"
                     /protein_id="HFD2358698.1"
                     /translation="MPLTQTQRLINTYGASLKNGTVSNEELIILLDPSTFTKSEGYVD
                     PNAPVSDSNHSKMDAIKDFVLTIGPTLDFEILHQLTSRMIELSPPGDRNTFMRGSSLE
                     KAFLAFEMAHYPTKAEEHFNSTRVRTEFPGENDIDNLKAVILNPIIAFFQSPPGIDKK
                     LEKLIKLCTDYKEHLKLSLTDDANNLVNKKHAIVTNMLAALTDSTAPNSTTKIKNMEQ
                     ILTEENKTTLKDRRDSFSGARFLESILHILSLGIYSKLTKGTFKFWKSHGEALSDNIE
                     EVSKQQPDMK"
     gene            8310..8510
                     /locus_tag="ACF2SP_000897"
     CDS             8310..8510
                     /locus_tag="ACF2SP_000897"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD2358699.1"
                     /translation="MKKQVQVIDIKQNLDMKQHDTNSKIMITLFSIFAELECDFIILR
                     TKEALASKKMQGIQLGKPNGTI"
     gene            complement(8820..9698)
                     /locus_tag="ACF2SP_000898"
     CDS             complement(8820..9698)
                     /locus_tag="ACF2SP_000898"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2358700.1"
                     /translation="MELRHLRYLLAVYEQRNFTRASELLYVSQPTLSQQIGDLEEELG
                     CLLFHREYHQLIPTEAAEIACNYAKEALGIVQELKNSLLEMQGLQRGCLKLGVIQTFN
                     AFYLSEILSQFLEKWPEIDIEVEELANNDISKAVLAGRLHLGIGFTASDNRMHKHKLY
                     DEDILIACNTRHRFAQYSEINIQELADEMFLTLPQQFTIRQWVDELMAKHRTVPRRII
                     EFNTIIAILSTLHKINGVAVLPAVTKRALPNKGLHFARLLPALPMRSVSLHRLPSSNC
                     IPLVSAFEQHLLLSFK"
     gene            9811..10290
                     /locus_tag="ACF2SP_000899"
     CDS             9811..10290
                     /locus_tag="ACF2SP_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFD2358701.1"
                     /translation="MSVMKKFHSADFDKTPPTIVVELPEKLKHQNVEGNGKNTNYSTA
                     RKHPVHFVDLPSHAISITIGSLDPDGRSNRHRHTYETILFVLEGKGYSVIEDTCVEWE
                     KGDAIYIPVWAWHHHVNQDNQKSAKYLACENAPMLQNLGCLAIREESHLTIGNNNNV"
     gene            10283..11182
                     /locus_tag="ACF2SP_000900"
     CDS             10283..11182
                     /locus_tag="ACF2SP_000900"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFD2358702.1"
                     /translation="MFNPPKGIIAYAVTPFSKDDNSLDLGAFDRVTEHLLFTAPSAIS
                     FLGSTGESAYLSEEEWQLVALKGVEKAANHTPVIIGISELTTSAAIRKAQYVEKIGAD
                     ALMIIPISYWKLSEQEIFDYFAAIAEKTSLPIMAYNNPSTSGLDMSPALLLKLAREID
                     NITMIKESSGDIQRMHTLFYESDGEIPFYNGSNILALEALAAGAIGWCSAAPNLIDRQ
                     PAALYETMAMGNLQESRKLFYSMLPLLRFIVNGGLATTVKAGLILRGLNAGDPRHPLK
                     PLVGKSLEELRRLMSANAIKVGV"
     gene            11182..11874
                     /locus_tag="ACF2SP_000901"
     CDS             11182..11874
                     /locus_tag="ACF2SP_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570167.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFD2358703.1"
                     /translation="MKIQLIQHAPFENPGAIVNWAIERGYQINSTHTYKEEKLPDLSE
                     IDFLIIMGGPQSTMELDKYPYLRDEIALIRQAINSKKTVLGICLGAQLIAESLGAKTE
                     PSPNKEIGVFPVQLTNEGEQDPIFGFFPKIFDVMHWHNDMPAIPAGAVLLAASNGCPR
                     QAIRYGDRVYALQFHMEMTKELVQNMISNCPNDLGPGNYIQDVLQLLSIDFTELNKKM
                     YCVLDYLSALAK"
     gene            11936..12079
                     /locus_tag="ACF2SP_000902"
     CDS             11936..12079
                     /locus_tag="ACF2SP_000902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358704.1"
                     /translation="MRIRKVQLGTPESHQNIVLNSLDYFLHNGCDDNGGMPIEYWIFL
                     ANK"
     gene            complement(12269..13792)
                     /locus_tag="ACF2SP_000903"
     CDS             complement(12269..13792)
                     /locus_tag="ACF2SP_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFD2358705.1"
                     /translation="MRKKPLSTGAEWTFELIQAYDREIARIAKSFNLDTYPNQIEIIT
                     AEQMMDAYSSVGMPIGYHHWSFGKHFVSVEKSYKRGQMGLAYELVINSNPCISYLMEE
                     NTMAMQALVIAHACYGHNSFFKNNYLFKMWTSADAIIDYLVFAKKYISDCEERYGIDA
                     VEPVLDACHALMNYGVDRYKHPTPLSIQEEKIRQQNREIYMQSQVNELWRTIPFSKQI
                     DKNGQKKRFPEEPQENILYFIEKNAPLLEPWQREIVRIVRKLAQYFYPQGQTKVMNEG
                     WACFWHYTILHALYDEGLVTDEFMLEILQSHTNVIMQPAYNSPYYNGINPYTLGYHMM
                     QDIKRICENPTDEDKKWFPYLANTDWLTSLDTAMRNYKDESFIAQYLSPKLIRDLKLF
                     HIVDDDRNSELYINAIHDEWGYQQIREALSRQYNLGYLEPDIQVYSVDLQGDRSLTLR
                     YSQQNRVPLGSSTNEVLKHLHYLWQFPVILQAVDGENKITEEFSCPPLNTNKTGETN"
     gene            complement(13789..15054)
                     /locus_tag="ACF2SP_000904"
     CDS             complement(13789..15054)
                     /locus_tag="ACF2SP_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFD2358706.1"
                     /translation="MSQLIDRRQNAGKKSTVNRQRFLRRYKSQIKKAVSEAVGKRSIT
                     EIDQGEQITIPAKDIYEPQFHRGHGGHIERVLPGNDNFIAGDRIKRPSGGGAGGAGGN
                     ASDSGEGEDNFVFELSREEFLELYFEDLELPDLVKKELARISTYKTVRAGVTTSGIPN
                     NINVLRSMKQATGRRVALASPYKRRLKEAEEELERLKQLANPDKIDLLKLERDIEFFK
                     KKIQTVPFIDTIDLRYNHRVRVPSPSTQAVMFCVMDVSGSMDEAKKDIAKRFFILLYM
                     FLTKNYEKIELVFIRHHTSAKEVNEEEFFYSRETGGTVVSSALELLNTIIEARYPPQA
                     WNIYVAQASDGDNWNADSPYCQELLQEKIMPLLQYFAYIEIMPRHHQSLWEVYQQVKE
                     RYPNFAMENIDNVADIYPVFRELFKRKTV"
     gene            complement(15208..17139)
                     /locus_tag="ACF2SP_000905"
     CDS             complement(15208..17139)
                     /locus_tag="ACF2SP_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HFD2358707.1"
                     /translation="MNTQDFLAGYTKRFVDNKEEDMSLDEYLELCKTDPTAYANPAER
                     LLMAIGEPELIDTRHDPVLSRIFSNKIIHHYEVFKDFYGMEEPIEQIVGFLKHAAQGL
                     EETKQVLYLLGPVGGGKSSIAEKLKDLMERIPFYAIKGSPVFESPLSLFNPLEDGELL
                     RERFGIPTRYLRYLMSPWAVKRLQEYNGDISKFRVIKVKPSRLKQIAIAKTEPGDENN
                     QDISSLVGKVDIRKLEEFSQDDPDAYSYSGGLCRANRGLLEFVEMFKAPIKVLHPLLT
                     ATQEGNYNATEGLSAIPFEGIILAHSNESEWQSFRNNKNNEAFIDRINIVKVPYCLRV
                     SEEIKIYQKLIHNSSLANAPCAPGTLDMLAQFSVLTRLKEPQNSSIYSKMRVYNGESL
                     KDTDPKAKSYQEYRDFAGVDEGMSGISTRFAFKILSKVFNFDHTEVAANPVHLMYVLE
                     RQIEQEQFPQELHETYLNFIKEYLATKYVDFIGKEIQTAYLESYSEYGQNIFDRYITY
                     ADFWIQDQDYRDPDTGEIFDRALLNLELEKIEKPAGISNPKDFRNEVVNFVLRARANN
                     RGKNPVWNSYEKLKSVIEKKMFTNTEDLLPVISFNAKASEEDKKKHEEFISRMVEKGY
                     TRKQVRLLCEWYLRVRKSQ"
     gene            17312..19903
                     /gene="pepN"
                     /locus_tag="ACF2SP_000906"
     CDS             17312..19903
                     /gene="pepN"
                     /locus_tag="ACF2SP_000906"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFD2358708.1"
                     /translation="MPDTTIYLKNYQPPSFTVETVELNFDLYDDHALVNSTLKLTRQK
                     DGALHLYGEELELIALHLNGRPLEHGEYVLMKDALIIEQCPDQIVLNIVTRIKPQENT
                     QLSGLYRSNHLFCTQCEAEGFRRITYYLDRPDVLATFTTRITADKKQYPILLSNGNLM
                     DTGETADGRHWVIWEDPFKKPSYLFALVAGNLACVRDSFVTCSGRTVDLRIYVEPGNE
                     DKCSHAMESLKKAMKWDEEVYGREYDLDIYMIVAVSDFNMGAMENKGLNIFNSKYILA
                     RPETATDQDFADVEGVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSRDMNS
                     RDVNRIMDVKVLRSAQFPEDAGSMAHPVRPESYQEINNFYTATVYNKGAEVIRMQHTL
                     LGKERFRRGMDLYFKRHDGHAVTIDDFVAAMEDANGVDLTQFKFWYSQAGTPEVRVLS
                     HFSEGRLEITMQQNCPPTPECHEKKPFHIPVRIALFDLKGEMIPIENELLELKEKEQR
                     FIFSGLNEKPVVSLLREFSAPVKIYDDLDQDDLLFLLRYETDGYAKWDAAQRLVLNCL
                     NENLKLPASEWQISKTLVAAFQHVLLDDSLDMDLRAELLTPPGFEEVAATMKWVDVSK
                     VESVRDYFRQQLGLGLYTKASDLYKELWEVEDHRMHGQAYGRRKLRNVCLWLMMKAKE
                     SDALELCREQFKTSRTMTDQIASCSLLVNCSRESHRNEAIEGFYKQWSHNELVLDKWF
                     AMQAACELPGTLDHVKKLTQHPAFCIKNPNKVRALVGAFCMANPRNFHALDGSGYVFL
                     SEVLIKIDKLNPQIAARLATPFTRWRSYDEPRQKLIQNQLEQLTKLDLSRDLREVVDK
                     SLIVEEK"
     gene            20127..20924
                     /gene="aroE"
                     /locus_tag="ACF2SP_000907"
     CDS             20127..20924
                     /gene="aroE"
                     /locus_tag="ACF2SP_000907"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFD2358709.1"
                     /translation="MLRRFAVIGNPIAHSLSPVIHQMFAQQTQIELIYEKILGDDVKF
                     EQQISDFFIQYGNGLNVTLPYKKRAYELAKIRTQRCTLAGAANTLWMEENQLHADNTD
                     GVGLIRDLSRFLELKDKKILILGAGGAARGIIFPLLEAKPLQLIVANRTLEKAKELQR
                     QFPQINVASFAELTEFFDLIINATSASLSDQVIVLPEEVLSHKPFCYDLAYNQKTSTA
                     FVQYTRNGGCEAVDGLGMLVEQAAEAFFIWNKVMPSTQKILEHLRSF"
     gene            complement(20974..22086)
                     /locus_tag="ACF2SP_000908"
     CDS             complement(20974..22086)
                     /locus_tag="ACF2SP_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2358710.1"
                     /translation="MMVKKALYLAGGGARGAYQAGVLKAIGHILQTKTLPFDLVSGVS
                     VGSVNAAVLAENANDFPAALEKLEAIWGEIHCQQIYKASNYELSKSVMRNLSTLIIKQ
                     RQSGHLLDTTPLRQFLEESIDFTRIEQNIQEGYLDTFEVLSACYETHQTISFYAHNHS
                     EFEDWNYPRHSSQRAVINAQHILASSALPLFFPPVSLNGFHYGDGSIGLVAPLRGAIR
                     FQVDRILILGTRELPEFTDPETLRNGEIAFAHILGNMMNGLFLDNLDRDIEMVNRMNE
                     IATLLSMWKKRRSPWRPISTLHLRPSRAMASVAQSHYTSMPVLLRFLLNIMGARSHSG
                     DLLSFLLFEKEFTRELLELGYQDTIAASAEVSAFFS"
     gene            22224..23606
                     /locus_tag="ACF2SP_000909"
     CDS             22224..23606
                     /locus_tag="ACF2SP_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358711.1"
                     /translation="MGITKDERFRKFEIEKTLQLLQSMQRPIEYPLTPIDKTSLLKKA
                     TKRIPLLSKFLKTVDDTGEVVVKIGDRMTSLSHTPGKESLAHGFAFGGVAMAGFDFLR
                     IPVIYLSAYLLGEEVPVNLSNNAKWFYSALLLGLTITALAVPVTAPYIAFAIAGLSLG
                     VGTFLLGKTLYERYQLGRERKKLKAEIIREEDEMLKIQDKAKHLESLLQSADNEEQIA
                     AILYEISVVQEDYNRQKERIQSLKNDELQLEQQIKKLGTMKVVDRTVAVALSALTIAA
                     LAVTLFFPPVGGGMLAGIAIFGGAYFVARVSTPLIKATAVWLYNKLKSTPSESPESQD
                     TDNKPTLSNEKEEKKEDSLQIDNEFVLPPIDAETSISEDSKEYKPVHESTEDVLVRLS
                     VNIDRLKEQVRTEKKEEIEEESPEELQHFSSLTPSPRTLGSRKLSAKENDDEDGESES
                     DIEHPDVPKI"
     gene            23674..25176
                     /locus_tag="ACF2SP_000910"
     CDS             23674..25176
                     /locus_tag="ACF2SP_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFD2358712.1"
                     /translation="MLNLFREQPRAFHMIFMLELWERFGFYTVQGILTLYFIRYLGYS
                     DLESYYTFGAYSALVYGMVVIGGYLGDRILGTKRTIVLGLVVLAMGYFSLAVTDKQHV
                     FLALGLICVGNGLFKANPSNLLSKCYDDNDPRLHGGFTLYYMAINLGSMVALFVGPAV
                     SSAYGYSYAYMISAIGLVLGIVNYWYQRQHVAHINTKADLRKITAFHWGIIIAGVLIL
                     TGISAYLLQHVMLAKNLLWGITLLVVSVYIYLMRKQAQASFMRMLVALVLMIEAVIFF
                     TLYQQMPTSLNLFAVNNVIPTFLGITIDPQSFQALNPIWIVLMSPVLAYFYRKLHQHN
                     ILFSIPYKFAFGMTMCAVSFLMLYFARYFYVGNGMVSSWWMIFSYLFQSLGELLVSAL
                     GVAMVAELVPAANAGFVMGMWFLTSAVAGFIGATVASFTALPENIKPGVESLLIYSDV
                     FACIGLVTLLIAVIMWLVSPRLSRYITVPSLEGDKDIETVEYSTTYIPSH"
     gene            25344..26750
                     /gene="lem29"
                     /locus_tag="ACF2SP_000911"
     CDS             25344..26750
                     /gene="lem29"
                     /locus_tag="ACF2SP_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem29"
                     /protein_id="HFD2358713.1"
                     /translation="MYKICTCNQSFPNHYWVKVLRFCELICLEYKVGLEKKIKLSQRS
                     LELLNDYLNLGGSGPSNLVNGSSSIDFSSLTQLLASNLSKSSKTSKTIKKKREFIEAA
                     LYAELLRDLSTSLNGSDEEIKPAEKEPPVSNKLKFWLLAIAGTLLAACEGFDSITTMM
                     AVLSFPAWVVLCAGILFSALSVVVFYGFDLVQVSQNLEIKLTDTPKLLDLYLSQMEEI
                     KAIRKKIDNYSLASMSMDELNELHLILVMLQDRVNSLVKAGQKFDLALNSPKMDVAKT
                     IVSGVSGILFFGGGFFAGQSVATFMLALVMTSVTPTFWPVLLFSVIVGLAAFSLYWYV
                     QRVGVNKLVSEWFGLDEDKIEKLCGKSKMDKELKKLNNLDEKVMTTSDLLSKVANLES
                     KLIDIGKQMKEMSAHQAPEKKLESATGLKASSNVYSFHFNSSNRDQESVYGERVLDDA
                     VSDYGRGQSHNDSIHSLC"
     gene            complement(26635..27435)
                     /locus_tag="ACF2SP_000912"
     CDS             complement(26635..27435)
                     /locus_tag="ACF2SP_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358714.1"
                     /translation="MNRHDVKAIHSYVVDEEKKKISLKLINKLYRFLSKYYHLVSQEE
                     FIRFIIQPDLYGELTVLYGENKEIAGFSRNCRQSVDLGKKQVTCYIAYLYLNPEYQIH
                     ASIKSAGLTQAIKYKLANPHEELVYIAFANNPLTYEFIYQLSDLIYPKPLQRVPDQIL
                     TVLNALKKQNGWISTNNHPMVVNSPLVPLRSQLSRINEESSELKEFYMSANPDYLQGN
                     SLLIYMPLHLANIGYGLNHYDSDPCHNRKQHHQGPSHRKRSPDPDLRN"
     gene            complement(27718..28410)
                     /gene="hda"
                     /locus_tag="ACF2SP_000913"
     CDS             complement(27718..28410)
                     /gene="hda"
                     /locus_tag="ACF2SP_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFD2358715.1"
                     /translation="MNKQLALAIKLNDEATLDNFNWGNNILLQQQLIEMLSFKADRLL
                     YLWGPKGSGKSHLLQACCQAINLTQSAIYLPLAFLKEWGPQSIEGLEDQTLICIDDIN
                     TIANDSAWEEALFHLYNKIKDSERSLLIISGNQPPIKCDIKLADLRSRLSWGLVIQLN
                     ELNDDEKINTLKMRAAKRGFELPESVGHFLLNRCSRNMHDLYELLNRLDDASWEAHRK
                     ITIPFVKNILKI"
     gene            complement(28407..>28848)
                     /locus_tag="ACF2SP_000914"
     CDS             complement(28407..>28848)
                     /locus_tag="ACF2SP_000914"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFD2358716.1"
                     /translation="GLTDGLDGWLARHFQWQSFFGSFVDPLADKLLVASSFISLALLN
                     SLPWWLVLLVFLRDFTISMGVLAWYYFIQRKLDFEPSRLSKLNTGFQLTLVTLCLFEL
                     AYFRFPYDLVDILIYLTAFTTVTSYLDYVWTWSRKAWSPKESLK"
BASE COUNT         8594 a         5155 c         5956 g         9143 t
ORIGIN      
        1 tgctagacta atagtaagag gtaaatatgg ggggtattta tgaaacaact aatattacat
       61 cccactgata ttagtcaatg gcatgcgctg gttaatgaag cgcaagcagc taccagactg
      121 atattgaatg aaaacacgga aagttatttg gtttttttgc tgatgcgctt ttctcaaggc
      181 acaaagctta tcgaatcggt tgttgcactt gattttcttg aatccatgcg caaacctcgc
      241 cagttacaag tcgaattatt aagggatgtg ggggacaaaa gcttgttgtt ttgcggctta
      301 tttccaggta tcgctaaaaa acgccatgtg agtttgagtt atttttctga catggggcaa
      361 gcggcttatt tgactgtcgg agagcttgag gaaaatcaga cagccaattt gtatatgcaa
      421 ttaagtgagc aatttattac tttgcagcaa gtgctacaag ctatgcgagg agagtatatc
      481 cagtttagtc agtcaagttg tggagttctg gcagaacccg atttgactat gcagtaggca
      541 gaggctcatt ttatttgcga aatagtagag tcacgtcgat tgaatcaaaa gtgtattttt
      601 gattgcaaaa atcgcaagtg atttctattt ttcctttttc tttaattaaa tctgttgctt
      661 cttcttctcc caggacagta atgacctgtt tcattttgtc ctgattacag cggcattgga
      721 aacgggtaga tcggctttcg aatattctta attccgtttc atgataaaga cgatagagta
      781 gtgtttgatt gtctatggtc agcaattcat cttcagttac tgtttgtccc agctgaacgg
      841 catactccca aaattgttct ctttgaattg tatcttgacc tggcataagt tgtaacagca
      901 tacctgcagc catatcttca ttgacagcta accacacgcg agtagcaatt tgttctgatt
      961 gggcaaaata agtcatcaga ttttcgctca tggacgtgga ttgaataggt atcaggcttt
     1021 gataggcttg agtttggttg tcttgactaa gcgtaatgac cattcggcct tgaagaaaag
     1081 cggtcgcgta gtcagcaatt tcaagaccct ctgcacattt tgcaaagccc cttatattta
     1141 aattatgatc gcattgaacg agtaacaggg ataatctttc gtcaccttga aattgcaaac
     1201 ttaaatttcc ttcaaattta atgctgcttg ccaggagtaa acatgagact aacgcttcac
     1261 ctaaaagatt ttttaccatg ggtgggtaat cacgttggtt catgatgttt tgaaatgttt
     1321 tttcaatatg aacgatttcg ccacggatat tcgcatgctc aaaaataaaa cgctgtaagg
     1381 tatctgcttc tttcatttca aactcataac acaaataggc ctattctaac ataaaatgtt
     1441 tatctcaggc cttttctgag ttcagttatt ttgaagaaaa tcttacaaga ggaataatag
     1501 tttccataac cagcgtatct taattatttt ttaattttcc tggagaaatt tatttcatgg
     1561 actattctta aaaaacccct ttttaaaatt gtaaggagtt attatgaaaa ataacagttt
     1621 acttcttccc ctatttaaaa caatagacaa attactgttt ggtatagaag aaattaaaga
     1681 aatagaaaaa acaccagaat atattctctg tgacaacgaa gatgagtttg atgtagaacg
     1741 atctatcaat taattcttgc gtgaaaaaga aagcttaggt gataatgcct tttttttgag
     1801 cggaaataag tatgttgaat agccaacaaa tggctgcagt aaaatatatt gatgggcctt
     1861 tgttggtgct tgccggggct gggagtggta aaaccagagt tattacacaa aaaattggtt
     1921 atttaattaa ttcatgtggt tataatgcca actccatctg cgccgttact ttcacgaata
     1981 aagcggcaaa tgaaatgcgc gcaagggttt cggctttttt acctgccgta agcaggcggg
     2041 gattaaaggt tgcgacattt catacattag gcctaagcat catcaagcgt gatgtttctc
     2101 ggtgcgattt gaaaccggga ttttccattt ttgatagtga agattgtttg caaattttgc
     2161 ggggtttttt acctgttaat aaggcaaccg acagagattt tatcctcaaa gttcaacaac
     2221 aaatttctct ttggaaaaat aatttattga atcctgaatt aatactccaa aatcctccag
     2281 aaacactttt atgtgacgag gcgttaacaa tttatccacg ctatcaagaa gcattgaaag
     2341 catataatgc agtggatttt gatgatttaa ttcggttgcc tgtcaatcta ctgaatgatc
     2401 atgctgatgt gttggaatat tggcagaata aaattcgtca tttgttggtg gatgaatatc
     2461 aggattcaaa cactagccag tatcttctgg taaaaaaatt ggcaggggtt cgtgcccact
     2521 ttacggttgt gggtgatgat gatcaatcga tttatgcctg gcgtggtgcc aaaccagaga
     2581 atttggcgca gttacaaaaa gattttcctc ggttaaagat tattaaatta gaacagaatt
     2641 acagatcaac cagccgaatt ttacatgctg ccaaccatct gatcgctcat aatcaacatt
     2701 tatttgaaaa aaaattatgg agtgatttgg ggcatgggga attattaagg gttgtcagct
     2761 gtaaggatga gcaggatgag gcggaacatg tgatcgctga tttaataagt cataaattac
     2821 gtaatcggac aaattatagc gattacgcta tcttatacag aggaaatcat caagcaagaa
     2881 tatttgaaaa ggtattaaga catcacggta ttccttatca tatcagcgga ggccagtcct
     2941 ggtttgccaa gttagaggtc agggacattt ttgcctatct taaattactt tgtaatgaag
     3001 cagatgatgc cgcttttctg cgagcaatta ctacccccaa acgaggtata ggagagagca
     3061 gtctagatgc tttggggcgc tatgctcagt ccagagggat aagtttatat cacgggtccg
     3121 atcatttggc cttgtctgag tttgttgctg acaaacagag aatgattttt catgatttca
     3181 aattgtggat ggaagacatt aaaaaacgta tcagttcaga ttcagttctg gagcatttaa
     3241 ggcaaatggt ggaagatatt gggtatgaag cttatattta tgaacaatgt gattctcccg
     3301 ccaaggcaca aaaaaaaatg gataatgtat gggagctttt agaatgggtt aatcgtttgt
     3361 taaataagga acctgaacaa tcgctcatgg atgttgtgaa taaattaata ctaatcgata
     3421 tactggaaca atccgatgaa tcttcaagtg atgtagtcca attaatgaca ttgcatgcct
     3481 caaaaggttt ggaatttcct tttgtttatt tggtcgggat ggaagaagaa ttattacccc
     3541 accgagtgag tatcgatgat gaccaaattg aagaagaaag aagattggct tatgtaggga
     3601 tcacacgtgc ccagaggggg ttatgtttta ctttagcgaa gcagagacgt cgagctggag
     3661 aactgcaaga ctgtgttcca agccgatttt tggatgaatt gccgcaagag agcctggaat
     3721 ggttcggtaa aagcggggag cgttgtgaag aacaatcaaa aaaactggcc aaatcccacc
     3781 tggaaggcct gaagaatttg ttgagttaag agtgcattat attttatctt cagtaaggag
     3841 tatcattaac tgaactcctt actgcttttt aaggtactaa tgttaatctc tatcatttgc
     3901 cagaagagag attgattcgt tctggaagct cttcaatgaa aacttgtgta cgggtttttt
     3961 cccctttttg gttaaatagt ctgtgttgat tgtagtctgg ttgcttttta gcctgctcca
     4021 gagtgtcact caatgtttct cctttttcag gatggcgtat tttttctttt aaatgattga
     4081 gggcgatgac ttttttatcg gctaattcgc gaccgataac gatgtctttg agatgctttc
     4141 tttgaaattt gtcgatttga gcaacagcat gttccacttg ccattcttgc gatgggcgtt
     4201 tggcatgagt acttaattca acattgaact ctttggctac tccccattgt ttattccatg
     4261 cttctccaag acttaacaca tgaggttttt tgccagaagg caaattctta ctatgattct
     4321 cttgaggagg atttagctta ctggcagaac aatcccagag tgctgccaag ccatatatag
     4381 gtgaagcaat ggtttttaat atccaggtag gaatatccat attatgatct tgatcggagt
     4441 caggatccag cctttccttt aggagttttt caaattggtg atgatcttgg tgtccatgtt
     4501 cttcatcctg ttcgtcttca tgggtatgtc caataaaata gtgggcatct tcaaagcctt
     4561 cgctaataat ggcaacgagt gcggaaagaa tctgcggtac tccgggcata cgatccgatg
     4621 tgactgcaac gctgatcaaa tgccccagaa acaaaagaat gcgtagtggg gtaatggtta
     4681 attttaaaag aatgcgaaac gggttaacta tttgcaacca attttcagtt tcacgtaagt
     4741 gctgatatcc attgcttatt gcttcgtaaa ttttttggaa gacgttctta ttactacgag
     4801 ttgcttcatc gaccatatct agtgattcag ccgtgttttg tatgttaaag actatagcag
     4861 aggcgccagt tataatggga ttgataattc ccataataaa acttggcatt tttttcatcc
     4921 agtcaaataa agggcgggca tttgtagcaa ttgtccacca tgttccagca gtacagatgg
     4981 ttagggctac tgccagtcca acaagaaaaa cagcggtgga tgctataaat acattgcgta
     5041 cagttaaacc ttggcttaag tcatcgcgaa ttttattata ccatttgaca accgtgttat
     5101 tgttgatcat gtcggtaacg gcattgtagg tcagcattcc ataagcggct cctgcgacga
     5161 tggccatggg aaggatgata atgggccaaa atgtaaatgg aatggcggca aaaaacggaa
     5221 ttattgtaaa ggcttcaaca ataagatagg tgcttccaag ccccatgaaa aggccggcaa
     5281 ctaaactaaa tcctttgaca atatgaaatt gaaaatgacg cttttcccat cgttttatcc
     5341 attcgtcttg atcatttaaa gcaagccagt caagtagttc cctggcgtat ttggatgtgt
     5401 ttggagatgg attgttttta tctgaaaaca attgcagggc aaaccacttt tccatgtccg
     5461 atagtgtctt ttcaatttgc cgctttcttt ttttactgtc ttgattgagt tctttgtgac
     5521 caaattcagc caataatttt aactgtcttt tataatcttt gaaaaattgc gggcaatttt
     5581 cttcgttagt gtttggaaaa tgattcagca aatactcttt cgcaaagtga ttcttaagat
     5641 agttaaattt aaaaagctta ttaagagcgc cttttatgtt ttgcaaataa atttcaccct
     5701 cataagctac cgataacgca aaaccagcaa aagccaaggg taacacaggc caaagagcat
     5761 acatgcctcc aaagcttaaa aagccgagta tcaggcttgc tccaacagtt aataacaaaa
     5821 gaaacaagta ataagggatt ttttggggct taataggtat attttttata ttcattgtag
     5881 tatatttgct cattgatgcg taatgcagaa ctttatacca aatgaaaatg attatcaata
     5941 gcattaatat taaaaagtat tttttgtctt tgtttcaatt caacaataaa gtttgtttgc
     6001 aatattcgtt tcatcaggta gtatcaaaaa cgatttaaat aacttaggag aggatatgcg
     6061 ttttaaccaa tggatgacct gtatcacatc aggattaata ctcgccagta attcaagttt
     6121 tgcaacgacg tcgcctgttc atgagcaatt gcaagtccca caatgccttg ctgcaaaaat
     6181 aaccgtaccc cacaagattt tggctgagaa taaagaattc aaaattattg atgtactttc
     6241 atctgatgta gagactttga ctattctggc tgatcaagtc agttgcggcc attttgtgaa
     6301 tgtgagtcat aaattaacgg gaaccttagc ggcaaaccaa cagcaatctg ctcaaaagtt
     6361 actgcaaaaa aagctcgtta agccctttgg tgtatccaaa ttacataaag acgtttatga
     6421 aatcaagcat gaagaagaag tcaatgcagc actaaaagaa attgtctctg ataacatatg
     6481 gcaaactctg actcatatga catcttatta taaccgctct gcaacaaaag atacgggtgt
     6541 tgaaactgcc aattggttaa aatccaaatt tgaacagatg gccgttgagt atggacgaac
     6601 tgatacctcg accttttttg taaaaacagg gtggtataaa caaccttccc tggttaccgt
     6661 gattggcaaa gacatcaaag cccccgctat tgttattggt gctcatatgg atactcttga
     6721 tggccgtatg cctggagctg gtgatgatgg aagtggatca tcgagcatta tggaagccgc
     6781 acgggttatt ttgtcttcta aaacaacatt taagcgcccc atttatttta tttggtatgc
     6841 cgctgaagaa agaggtttgg tcggttcaca acacgtagtc caacactttc aggagcaatc
     6901 aattccagtg aaggctgtgg tacaatttga catgaccggt tatcgaaatg atgccaatga
     6961 tccaactatg tgggtattta ctgattacac agatagagat ttaagtaatt atttggccaa
     7021 attaattgat cattatattc atgtgccagt agattattca cgatgtggtt atggttgcag
     7081 tgatcatgcc tcctggaatg aagaagatat tcctgctgca ttcccatgtg aaaccagttt
     7141 cgctgatcac aacccataca tccatacttc ttctgataaa atggatttat tgaacctgga
     7201 acatatgacc aatttctcca aactcgcggt agctttcgct attgaactcg cttccgagta
     7261 actgttttaa tgcctctcat aaattttaaa tatttttgag aggctatttt ttaaagttag
     7321 tttattgata tcgattgata tttatttcat atcaggctgc tgtttactaa cttcttcaat
     7381 gttatcggag agagcctcgc catggctttt ccaaaattta aaagtgcctt tggtcagttt
     7441 tgaataaata cccagactca atatgtgcag gatactttct agaaatctag caccgctaaa
     7501 tgaatctcgg cgatctttta aggttgtttt gttttcttca gtcagtattt gctccatatt
     7561 tttaattttt gttgtgctat ttggggctgt gctatcagtg agagcagcta gcatgtttgt
     7621 aacaatcgca tgttttttat ttactaaatt attagcgtcg tcggttagag agagtttcaa
     7681 gtgctcttta taatccgtgc ataatttgat aagtttttct aattttttat cgattccggg
     7741 gggcgattgg aaaaatgcaa tgattgggtt caaaataact gctttaagat tgtctatatc
     7801 attttcgcct gggaattctg ttctcactct tgtcgagttg aaatgctcct ctgcttttgt
     7861 tgggtagtga gccatttcaa aagctaagaa agctttttcc aagctagagc ctctcataaa
     7921 tgtgtttcta tctccggggg gcgataattc gatcattcga gatgttaatt ggtgaagtat
     7981 ttcaaaatct aaagtggggc cgatggttaa tacaaaatcc tttattgcat ccattttact
     8041 atgattggag tctgaaacag gcgcattggg atcaacataa ccttctgatt ttgtaaaagt
     8101 gcttggatcc agcaaaataa tcagttcttc gttagagact gttccatttt taagtgaagc
     8161 gccataggtg ttaatcagac gttgagtttg agttaaaggc atggcagtga ctccaaagat
     8221 tgattacggc aaattatagt acaaaattat taagaaatta ttaggagagt atttctattg
     8281 aggcaatagg aatagggaat cacgaattca tgaaaaaaca agtgcaagta attgatatca
     8341 agcaaaactt ggatatgaag caacacgata cgaattctaa aatcatgatt accttgtttt
     8401 caatctttgc tgaactagaa tgtgacttca ttattctcag aaccaaggaa gcgcttgcca
     8461 gcaaaaaaat gcaaggaatt caattgggca aacctaatgg cacaatttaa aaaagtaaat
     8521 ttgataaaga tgctgaaaaa aattaaagag ctattagctt taggtttatc agttagaaaa
     8581 attgcaacgt attttgggca tagcaatcat attagtctta ataactattt ggggaaaaag
     8641 aaaattgcgg gaaataatta attctgtgaa tatttcttaa gccactacta aagttattat
     8701 attgtcgctt gaactagtta ttttagaaaa tcattcacat caatcgctta gatggtgcta
     8761 aatttacctg ctcagccgat ggtatggctt attacattaa aaactaaaag aacttccagc
     8821 tacttaaaac ttagcagtaa atgttgttca aaagcactta ccaagggaat acaattgctg
     8881 ctaggtagac gatgcaaact aacactgcgc attggtagtg caggtagaag gcgcgcaaaa
     8941 tgcaatcctt tatttggtaa agcgcgttta gtaactgcag gtagaacggc aacaccatta
     9001 attttatgta aggtgctaag aatagcaatg atggtgttaa attcaataat tcgtcgagga
     9061 acagttcgat gcttggccat taactcatca acccattgac gaatagtaaa ttgttgcggc
     9121 aaggttaaaa acatttcatc tgctagctct tgaatgttta tttcagaata ctgtgcaaaa
     9181 cgatgtcttg tgttgcatgc aatcaaaata tcttcatcat aaagtttatg cttatgcata
     9241 cgattatcag aagcagtaaa accgatcccc agatgtagac gaccagcaag aactgccttg
     9301 ctgatgtcgt tgttagctag ctcttcgact tcgatgtcaa tttcaggcca tttttccaag
     9361 aattgactga gaatttctga aagataaaaa gcattgaaag tttgtatgac gccaagtttt
     9421 agacacccgc gttgaaggcc ttgcatttct agtaagctat tctttaattc ttgcacaatt
     9481 ccaagagctt cttttgcata gttacaggca atttccgctg cctcagtggg aataagctga
     9541 tggtattctc gatgaaataa taaacatccg agttcctctt caagatctcc tatttgttgc
     9601 gataaggtgg gctgtgaaac atacaataat tcagatgctc ttgtaaaatt tcgttgttca
     9661 tagacagcga gtaaatagcg taaatgcctt aattccatct gtttagtctc atcataagtt
     9721 taacctatag caatataagt ctaaagtatt agaaatagca aacagtttat ttttatactt
     9781 cagttcttta attccagtag aggagagagc atgtctgtca tgaaaaaatt tcattctgca
     9841 gattttgata aaacaccacc aacaattgtt gtggaattac ccgaaaaatt aaagcaccaa
     9901 aatgtggagg gcaatggcaa aaatacgaat tattcaacag cacgtaaaca tccggtacac
     9961 ttcgtggatt taccttccca cgctataagc ataacaattg gaagcttaga tcctgatggg
    10021 cgttcaaatc gtcatcgcca tacttatgag acgatattgt ttgtattgga gggtaaaggc
    10081 tacagtgtca ttgaagacac ttgtgtagaa tgggaaaaag gggatgcaat ttatatccct
    10141 gtttgggcat ggcatcacca tgtgaatcaa gataaccaaa aatcggcaaa atatctggct
    10201 tgtgaaaacg cgcctatgct gcaaaatctt ggctgtctgg caattcgcga agagagtcat
    10261 ctaactatag gaaataacaa taatgtttaa cccacctaaa ggaatcattg cttatgctgt
    10321 tactcccttt tcgaaagatg ataacagtct cgatttaggc gctttcgatc gagtgacaga
    10381 gcatttactc tttactgctc cttctgctat cagttttttg ggaagtactg gtgaaagtgc
    10441 ttatctgagt gaggaggagt ggcaactcgt tgctctcaag ggtgttgaga aagccgcaaa
    10501 ccatactcct gtcattattg gaataagtga gctaacaacg tctgctgcaa tccgtaaagc
    10561 tcaatatgtt gaaaaaatag gtgctgatgc tctcatgatt attcctattt cctattggaa
    10621 attaagcgaa caagaaatat ttgattattt tgcagcgata gcagaaaaaa cttctttacc
    10681 aataatggct tacaataatc cttctactag tggtttggat atgtctccgg ctttgttact
    10741 caagttggca cgagaaatag ataacattac catgataaag gaaagctccg gcgatattca
    10801 acggatgcat accctctttt atgaaagtga cggtgaaatt cccttttata atggtagcaa
    10861 tatcctggcc ctagaggctc tggctgccgg tgctataggc tggtgtagcg ctgcaccgaa
    10921 tttaattgat aggcaaccag cagccttata tgagacgatg gcaatgggaa atttgcagga
    10981 atcccgcaaa ttgttttatt caatgttgcc tttactgcgt tttattgtga acggaggatt
    11041 ggcaactaca gtgaaggcag gtcttattct tagaggactg aatgcaggtg atcctcgcca
    11101 tccacttaaa ccgttagtag gcaagtcact tgaagagctc agacgattaa tgagtgctaa
    11161 cgctataaaa gtaggtgtat aatgaagata cagcttatcc aacatgcccc ttttgaaaat
    11221 ccaggcgcta ttgtgaactg ggcaattgaa agagggtatc agattaattc cactcatact
    11281 tataaggaag aaaagctacc ggatctatct gaaatagact ttcttatcat tatgggtggt
    11341 ccgcaaagca caatggaatt ggataaatat ccttatttga gagacgaaat cgctttgatt
    11401 cgacaagcta tcaatagcaa aaaaacagta ttaggcattt gtttaggtgc acaactaatc
    11461 gctgaaagtt taggtgctaa aacagaacct agtccaaata aagaaatagg cgtttttcct
    11521 gtgcagctca caaacgaagg tgaacaagat ccaatttttg gttttttccc aaaaatattt
    11581 gatgttatgc attggcataa tgacatgcca gctattcctg ctggcgccgt attattagcc
    11641 gcaagcaacg gatgtccacg acaagcaatt cgatatggtg atcgagtgta tgcacttcaa
    11701 tttcatatgg aaatgactaa agagttggtg caaaatatga tttcaaactg ccctaatgac
    11761 ctagggccag gaaattatat tcaggatgta ttacaattgc tatctattga ttttactgaa
    11821 cttaataaaa aaatgtattg tgttctggat tatttatctg cattagccaa gtaaaaaaca
    11881 atacgagaca aggacgtcag gcaagttcag cataaatcga tgataaagaa accatatgcg
    11941 aataagaaag gtgcaactcg gcactccaga gagtcatcaa aatattgttc ttaacagcct
    12001 ggattatttt ttacataacg gatgtgatga taatggtggt atgccaattg aatattggat
    12061 ttttctagca aataaataaa aaacctacta attgcctcaa attaactgct gccgaaggag
    12121 gcaataagag tagggatagg taggccagga ttaggcggtt cgcagcaaat tacaggcagg
    12181 caagataaag ataagaaaaa ggatagacat gtgccctttt ggatgacagg aaactagtag
    12241 ctattttgaa tcaggttatg caagaggtct aattagtttc ccctgtttta tttgtattta
    12301 gaggtggaca actaaattcc tcggtgattt tattttcccc atcaactgcc tgtaatatga
    12361 ctggaaattg ccataaataa tgcaagtgct ttaatacttc attcgttgag cttccaagtg
    12421 gtactctgtt ttgttgggag taccttaaag tcaaagaacg atctccttgc aaatcaacgg
    12481 agtagacttg aatatcaggc tccagataac ccagattgta ttgtctggat aatgcttctc
    12541 tgatttgctg atatccccat tcgtcgtgaa tggcattgat gtataattcg gagtttcggt
    12601 cgtcatcaac aatatggaac agttttaaat ctcgtataag ttttggtgac aggtattgag
    12661 cgataaaact ttcatctttg tagttgcgca tcgccgtatc aaggcttgtg agccaatcgg
    12721 tattcgcgag atagggaaac cactttttgt cttcgtctgt tgggttttca caaattcgtt
    12781 tgatgtcttg catcatatgg tagccaaggg tgtatggatt aataccgttg taataagggc
    12841 tattataggc tggttgcatg atgacattgg tatgactttg cagtatttcc agcataaatt
    12901 catcagtcac taaaccttcg tcatacaaag catgcaaaat agtataatgc caaaagcagg
    12961 cccacccttc attcatcact ttagtttgtc cctgtggata aaaatattgg gccagttttc
    13021 ttactatacg cactatttct ctctgccaag gctctaataa aggggcattt ttttctataa
    13081 aatagagaat attttcctga ggctcttctg gaaagcgttt tttttgtccg tttttatcga
    13141 tttgcttact gaaaggtatc gttctccaca gctcattaac ttgcgattgc atgtagattt
    13201 cacggttttg ctgtcgaatt ttttcttcct ggatagataa gggtgtggga tgtttgtacc
    13261 gatcaacccc gtaattcata agagcatggc aagcatcaag cactggttct accgcatcta
    13321 ttccgtagcg ttcctcacaa tcgctgatgt atttcttggc gaataccaga tagtcaataa
    13381 tagcatccgc agaagtccac attttaaata agtaattgtt cttaaaaaaa gaattgtggc
    13441 catagcatgc gtgagcgata accaaggctt gcatagccat ggtattttct tccattaaat
    13501 aagaaataca gggattggaa ttaattacaa gctcataggc caagcccatt tgtcctcttt
    13561 tgtagctttt ctcaacactg acaaaatgtt ttccaaaaga ccaatggtgg taacctatag
    13621 gcataccaac cgaagaatag gcatccatca tttgttcagc ggtaatgatt tcaatttggt
    13681 ttggataggt atccaaatta aagcttttgg cgatgcgtgc aatttcccga tcataggcct
    13741 gtattaactc gaaagtccat tctgcaccag ttgatagagg cttttttctc ataccgtttt
    13801 ccttttaaat aattcgcgaa ataccggata aatatcggca acattatcaa tattttccat
    13861 ggcaaaatta ggataacgtt cttttacttg ttgatatact tcccacaaac tttgatgatg
    13921 acggggcata atttcaatat aggcaaaata ttgcaataaa ggcataattt tttcctgcag
    13981 caactcttga caataaggag agtcggcatt ccaattatcg ccatctgatg cttgggcaac
    14041 ataaatattc caggcttgag gagggtagcg tgcctcgatg atggtgttta atagctccag
    14101 ggcactggaa acaacagtcc cgccagtctc gcgcgaataa aaaaattcct cttcattaac
    14161 ttctttggct gaggtgtgat ggcgaataaa aaccaattca attttttcgt aatttttggt
    14221 aagaaacata tagagtagga tgaaaaagcg ttttgctatg tctttttttg cttcatccat
    14281 ggaacctgat acgtccatta cgcaaaacat cactgcttgt gtagatggtg aaggcacacg
    14341 cacacggtga ttataacgca gatcgattgt atctataaat ggtacagttt gtatcttttt
    14401 tttgaaaaat tcaatgtctc tttcaagttt caataagtct attttgtctg gattggctaa
    14461 ttgtttcagc cgttcaagct cttcttctgc ttcctttaat ctccttttat agggggaggc
    14521 taaagcgaca cggcgtcctg tcgcttgttt cattgagcga aggacattaa tgttgttagg
    14581 tattccactg gtggttacgc cagctcttac tgttttataa gtgctaattc ttgctagttc
    14641 tttttttacc aaatcgggta attccagatc ttcaaaatag agttccaaaa actcttctcg
    14701 agataattca aaaacaaaat tatcttcgcc ttcaccacta tcgctggcgt tacctccagc
    14761 tcctccagca ccaccgccgc taggtctttt tatccgatcc ccggcgatga aattatcatt
    14821 tcccggtagt acacgttcga tatggcctcc atggcctcta tggaattgag gttcataaat
    14881 gtctttagct ggtatggtaa tttgttcgcc ttgatcaatt tcagtgatac tccgttttcc
    14941 gacagcctca gaaactgctt ttttaatttg gcttttatag cggcgtaaaa aacgttggcg
    15001 attaaccgtg cttttttttc cagcattttg tcgtctatca attaattgcg acataattgt
    15061 tagctccaca ccttctgcag gtcagttttt tgttagccag gttgtaagca gtatctgaat
    15121 ttaggattct acttgatctt ttcagatagt tacttattaa ccctgactgt atttcataac
    15181 ctatcgagtt atgaatgatc ctacctatta ttgtgacttc cttactctca aataccattc
    15241 acaaagtaag cgaacttgtt ttcgtgtgta gcctttttct accattcgag agataaactc
    15301 ttcgtgtttt ttcttatctt cctcagatgc tttggcattg aaagaaataa cgggtaacaa
    15361 atcttccgta ttggtaaaca tttttttctc aatcactgat tttagttttt catagctatt
    15421 ccatacaggg ttttttcctc ggttatttgc tcgagcacgc agtacgaaat tgactacttc
    15481 attacggaag tctttaggat tggagatccc tgccggtttt tctattttct ccaattccag
    15541 gtttaataag gccctgtcaa atatctcgcc agtatccgga tcgcgataat cctgatcttg
    15601 aatccagaaa tcagcatagg taatgtaacg gtcaaatatg ttctgcccat actcagaata
    15661 ggattccagg taagcggttt gaatttcctt gcctataaaa tcgacgtatt tggttgccaa
    15721 gtattcttta atgaagttca agtaggtttc atgtaactct tgaggaaatt gttcctgctc
    15781 gatttggcgt tccagcacat acattaaatg aaccgggttg gctgccactt cagtatgatc
    15841 aaaattgaat acttttgaaa ggattttgaa tgcaaaccgc gtggatattc cgctcatccc
    15901 ttcgtcaacc cctgcaaaat ctctatactc ctgatacgat tttgccttgg gatcggtatc
    15961 tttcaaactc tctccattat acacgcgcat tttggagtaa atactggagt tttgtggctc
    16021 ttttaaacgg gttaatacag aaaactgggc tagcatatcc agtgtaccag gtgcgcaagg
    16081 ggcattggct aaagaactgt tatggattag tttttggtag attttgattt cttcagaaac
    16141 acgcaggcaa taaggcactt tgactatgtt gattcggtct ataaatgcct cgttattttt
    16201 attgtttcta aatgattgcc attctgattc attggagtgg gctagtataa ttccttcaaa
    16261 aggaatagct gaaagccctt ctgtggcatt gtaattccct tcttgcgttg cagtcagcaa
    16321 aggatgaagg acctttatcg gtgccttaaa catttcaacg aactccaaaa gcccgcggtt
    16381 ggctcgacac aagcctccag agtagctgta agcatcagga tcatcttggg aaaattcttc
    16441 caatttacgg atatcgactt taccaaccag agaagaaatg tcttgattgt tttcatcgcc
    16501 aggttctgtt tttgcgatgg caatttgttt gagtcgagaa ggcttgactt taataacccg
    16561 gaatttgctg atgtcgccat tgtattcctg taatcgtttg actgcccagg gggacattaa
    16621 gtagcgtagg tagcgtgttg ggataccaaa acgttctcta agtaattcac catcttccaa
    16681 ggggttaaat aatgacaatg gagattcaaa gacaggagaa cctttgattg cataaaaagg
    16741 gattctttcc attaaatctt tcaatttttc tgcaatggaa gatttaccac cgccaacagg
    16801 ccccaaaaga tataaaacct gttttgtttc ctctaacccc tgtgcagcat gcttcaaaaa
    16861 gccaacaatt tgctcaatgg gttcttccat tccataaaaa tctttaaaaa cttcataatg
    16921 atggattatt ttatttgaga aaattcgtga tagaaccgga tcatgtcggg tatcgatcaa
    16981 ttcaggctcg cctatcgcca taagcaaacg ttctgctggg ttggcatatg ctgtcgggtc
    17041 cgttttgcac aactccagat attcatccaa actcatgtct tcttctttgt tatcaacaaa
    17101 tcgtttggta taacccgcta aaaaatcttg tgtattcatg cgccatcccc ttttacttaa
    17161 ccctgagaag gatataatgt gattctattt ttactctttc ctgtataaaa gcaaaaatta
    17221 cttcttttaa ttcatagtat agtagactaa ccacttaatt taatacataa atcaataaac
    17281 ttattcgttt tttttatagg aagcaatgac tatgccagac acgaccattt atttgaagaa
    17341 ttatcaaccg ccttctttta cagttgagac tgtggaattg aattttgatt tgtatgatga
    17401 ccatgctcta gtgaacagca ctcttaagtt aacacgtcaa aaggatggcg ctttgcattt
    17461 gtatggtgaa gagctggaat taattgcctt gcatttaaat ggacgaccat tagaacatgg
    17521 ggagtatgta ttgatgaaag acgctcttat tatagagcaa tgcccggatc aaatagtact
    17581 taatattgtg actcgcatta aacctcaaga aaatacgcaa ttatcaggac tttatcgttc
    17641 taaccattta ttctgtactc aatgcgaagc agaaggtttc agacgaatta cctattatct
    17701 tgatagaccc gatgttttgg caacttttac tacacgaatt acagcagata aaaagcaata
    17761 tccgatttta ttatctaacg gtaatttaat ggatactgga gaaaccgcgg atggacgtca
    17821 ttgggtgatt tgggaggatc ctttcaagaa gccctcttat ttattcgctt tggtagcggg
    17881 taatttagct tgcgttagag atagtttcgt tacttgctcc ggcaggactg ttgatttacg
    17941 gatttatgta gaaccgggga atgaagacaa gtgctctcat gccatggagt cactaaaaaa
    18001 agccatgaaa tgggatgaag aggtttatgg ccgtgagtac gatttagata tttatatgat
    18061 agttgcggtg agcgatttca atatgggagc aatggaaaac aaaggattga atatatttaa
    18121 ttccaaatac atcctcgctc gccctgaaac cgccactgat caagatttcg cggatgttga
    18181 aggtgtagta ggccatgagt atttccataa ctggacagga aatcgtgtga cttgccgcga
    18241 ttggttccag ttaagtttaa aagagggatt gactgtattc cgggatcagg agttttctcg
    18301 ggatatgaat tctcgtgatg ttaatcggat tatggacgtt aaagtattgc gcagtgctca
    18361 gtttcccgag gacgccggga gcatggcgca tccagtaagg ccggaatctt accaggaaat
    18421 taataatttt tatacggcga cagtatataa caaaggcgca gaagtgattc gaatgcaaca
    18481 tactttgtta ggcaaggaga ggtttcgaag aggcatggat ctttatttca aacgccatga
    18541 cggccatgcc gtaaccattg atgattttgt tgctgccatg gaagatgcca acggagttga
    18601 cttaacgcaa ttcaaattct ggtatagcca ggcagggaca cctgaagtgc gcgttctcag
    18661 ccatttttca gaaggacgat tggaaattac catgcaacaa aattgtcctc ctacgcctga
    18721 gtgccatgag aaaaagccat ttcatatccc ggttcgcatt gcattatttg atttgaaagg
    18781 ggaaatgatt cccatagaaa acgagctttt ggagttaaag gaaaaggagc aacgttttat
    18841 tttttctggg ctaaatgaaa agcctgttgt ctcgctatta agagaattct cagcccctgt
    18901 gaaaatttat gatgatctgg atcaggatga cttgttattt ttattgcgtt atgaaacgga
    18961 tggatatgcc aagtgggatg cggcacaacg cctggttctt aactgtctca atgaaaactt
    19021 gaaattgcca gccagtgaat ggcaaatctc taaaactctt gttgctgcct ttcagcatgt
    19081 tctgcttgat gactctcttg atatggatct cagggcagag ttgttgacac ctccaggatt
    19141 tgaagaagtg gcagctacca tgaaatgggt tgatgtcagc aaggttgaat cagtgcgaga
    19201 ttatttccgt caacaattag gattgggctt atacacaaag gcgtctgatc tttataaaga
    19261 attatgggaa gtagaagatc atcgcatgca tggccaagct tatggacgca gaaaattaag
    19321 aaatgtttgt ttatggttga tgatgaaagc gaaagaatca gatgctttag aattatgccg
    19381 agaacaattc aagacttctc gtaccatgac ggatcaaatt gccagttgca gcttactagt
    19441 caattgctcc agggaatctc accgaaacga agccatagaa ggattttata aacaatggtc
    19501 tcataatgaa ttggtactgg ataagtggtt tgcgatgcaa gcggcatgtg agttaccagg
    19561 taccttggat catgtcaaaa aactaaccca acatcctgct ttttgtatta aaaatccgaa
    19621 taaagttcgg gcgctggttg gcgctttttg tatggctaac cccagaaact tccatgcgtt
    19681 ggatggcagc ggatatgtct ttttaagtga ggtattgatt aagatcgaca aactaaatcc
    19741 tcaaatcgcc gcacgcttag ctacgccttt tacgcgttgg cgaagttatg acgaaccaag
    19801 acaaaagtta atacagaacc agttggagca gcttacaaaa ctggatttgt cgcgggattt
    19861 gcgcgaggtt gtagataaga gtctgattgt tgaggaaaaa taggatgaat taaagtgtgc
    19921 tggattttgc tatagacaga ttcagcacgc cttaaacgat ttttatcctt ttatttgtgc
    19981 aaacaaagct tacaaaattc ataaattaac ttttcagctt gagcatcggg gcgcgattta
    20041 agaacaactt gaattgatgt gcttgttgcg tgagaacagt ttataattag gaaaatcaat
    20101 gaaacctata ggataaagga gtttttgtgc ttcgtcgttt tgcagtgata ggtaatccaa
    20161 ttgcacatag tttgtctcct gtcattcatc aaatgtttgc ccaacaaacc caaattgaat
    20221 tgatttatga aaaaatcctg ggtgatgatg tcaagtttga acaacaaatc tctgattttt
    20281 ttatccagta tggcaatggg cttaatgtca ctttacctta taaaaagcgc gcttatgaat
    20341 tagcaaaaat acgcacgcaa agatgcaccc tggctggagc agcaaacacc ctatggatgg
    20401 aggaaaatca attacatgct gataataccg atggtgttgg gttgatacgt gatttatccc
    20461 gatttcttga gttaaaggat aaaaaaattc tgattttggg ggctggcgga gctgcaagag
    20521 ggattatttt tcctttattg gaagcgaaac cgttacaatt gattgttgct aatcggactt
    20581 tggaaaaagc aaaagaatta cagcgtcaat ttcctcaaat caatgtcgca agttttgctg
    20641 aattaacaga gttctttgat ttaatcatca atgccacttc tgcgagtttg tcagatcagg
    20701 tcattgtatt acctgaagag gtgttatccc ataaaccatt ttgttatgat ttggcttata
    20761 atcaaaaaac aagcaccgcc tttgttcaat atactcgtaa tggagggtgc gaagcagttg
    20821 atggattagg tatgttagta gagcaagctg cggaagcgtt ctttatatgg aacaaggtta
    20881 tgccttctac ccaaaaaata ttagaacacc tgcgatcttt ttaaactgga ggcttgcata
    20941 acagttagat gtgctttggc aaccaaactc tggttaacta aaaaaagcgc tgacttctgc
    21001 ggatgcagca atggtgtcct gatatcctaa ttccagtaat tcccgagtaa attctttttc
    21061 aaagagtaaa aagctaagca gatcaccgga atgactcctg gctcccataa tattcaataa
    21121 aaatcgaagc aatactggca tgctggtgta atggctttgg gctactgagg ccattgcccg
    21181 gcttgggcgt aaatgcaggg ttgagattgg tctccaggga gagcgtcgtt ttttccacat
    21241 agataagagt gtcgctattt cattcatacg attgaccatc tcaatgtctc tatccagatt
    21301 atccagaaaa agcccattca tcatattgcc tagaatatga gcgaaagcaa tttcaccatt
    21361 gcgcaaggtc tctggatcag taaactcagg caattccctg gtacctaaga tgagaattct
    21421 atccacttga aaacgaatag caccgcgaag gggggctacc aagcctatac ttccatcacc
    21481 ataatggaat ccattgaggc tgacaggggg aaagaacaat ggtaaggcgc ttgatgccag
    21541 aatatgctgg gcgttgatca cggcacgttg gctgctatga cgcggataat tccaatcttc
    21601 aaattctgaa tgattatgag cgtagaaaga gatggtttga tgtgtttcat aacaggcgct
    21661 taagacttcg aaagtatcga ggtatccttc ttgtatattt tgttcaattc gtgtgaaatc
    21721 aatgctttct tccagaaatt gccgtaatgg tgttgtatca agcaaatgcc ctgattgacg
    21781 ttgtttgata atcaaggtgc ttaaattacg cattactgat ttgcttaatt cgtaattact
    21841 ggccttgtag atttgttgac aatgaatttc accccaaatg gcctcgagtt tctccagggc
    21901 agcaggaaaa tcattggcat tttcagcaag tacggccgca ttgacgctgc ctacgctgac
    21961 accactcact aagtcaaaag gtagggtttt ggtttgtaaa atatgaccga tagctttgag
    22021 tacgcctgct tgataagcgc ctcgagcacc accacctgcc aaatatagag cttttttaac
    22081 catcatgttt tattttattt attataactt gaaatatagt tgaaatctgg gctaattagc
    22141 aagaaagaat tgtcgatgtc atcttttctt aattatgaat tgatatactt aaattaaatc
    22201 gattcttttc ttgagtcatt atcatgggaa ttacaaaaga cgaacgcttc agaaaatttg
    22261 aaatagaaaa gactcttcaa ctcttacagt cgatgcaacg tcctattgaa tatccgttga
    22321 cgcctataga taaaacgtca ttattaaaaa aagccacaaa acgcattcct ttgctgtcaa
    22381 aatttttaaa aactgttgat gatacgggtg aagtagttgt caaaattggt gataggatga
    22441 ctagtttatc ccatacacct ggtaaagagt ctttggctca tggttttgcc tttggtggtg
    22501 ttgccatggc aggctttgat tttcttcgta ttcctgtcat ttatttgtcg gcttatttgc
    22561 taggcgaaga agtgcctgtt aatttgagta ataatgccaa atggttttat tccgccctat
    22621 tgttaggatt gaccataacc gcactagcag tcccggtaac agctccttat atagcttttg
    22681 ctatagcagg cctcagttta ggtgtgggta cgtttttatt aggaaaaact ctttatgaac
    22741 gctatcaatt aggtcgggaa agaaaaaaac taaaagcgga gatcataagg gaagaggatg
    22801 aaatgctgaa aattcaggat aaagcaaagc atttggaatc ccttttacaa agcgctgaca
    22861 atgaagagca aatagcagcc attttatatg aaatatcggt tgtccaggag gactataaca
    22921 ggcagaaaga acgcattcag agtttaaaaa atgacgagtt gcaattagaa caacaaatca
    22981 aaaagttggg tacgatgaaa gttgtggaca ggactgtcgc ggttgctttg tctgcgctta
    23041 ctatcgccgc tttagctgtg actttatttt tcccgccagt tggaggaggc atgttggctg
    23101 ggattgccat ttttggcgga gcgtattttg tggctagagt aagcactcct ttaattaaag
    23161 caacggctgt ttggctctac aataaattaa aatcgacccc aagtgagagt cctgaatcac
    23221 aggatacaga taataaaccg actttaagta atgagaaaga agaaaaaaaa gaagattctt
    23281 tgcaaatcga taacgagttt gttttaccac caatagatgc tgagacgtca atttctgagg
    23341 attcaaagga gtataaacct gtgcatgaat caactgaaga tgtactggtg aggttatcag
    23401 taaatatcga caggctcaag gaacaggtaa gaacagaaaa aaaagaggaa atagaggaag
    23461 agtccccaga ggagcttcag catttttcat ctctaactcc ctccccaaga acattgggat
    23521 ccagaaaact atcagcaaaa gagaatgatg atgaggatgg agaaagcgag tcagatatag
    23581 aacatcctga tgtccctaaa atttaaatct gcattgcaaa aaacaagaat ttcctttaag
    23641 ttaaaaaaaa ttactatcaa ggaatgaaaa ttcatgctga atttatttcg tgagcaacct
    23701 cgtgcatttc acatgatttt tatgttggaa ttatgggagc gatttgggtt ctatacagtt
    23761 cagggaattc ttactcttta ttttattcgc tacctgggtt atagcgattt agagtcttac
    23821 tatacctttg gtgcttattc tgcgctggta tatggaatgg tggttattgg tggctatttg
    23881 ggagacagaa ttctaggtac caagcgaacc atagttctcg gtcttgttgt gctggccatg
    23941 ggttactttt ctcttgcagt tactgataag caacatgtat ttcttgcact aggattgatt
    24001 tgtgtaggta atgggctatt caaagcgaat ccatcgaatt tgttatccaa atgttatgat
    24061 gacaatgatc cacgactaca tggcggtttt accctttatt atatggctat taatctgggc
    24121 agtatggttg cattattcgt cggaccagca gtatcaagcg cttatggtta ctcttatgct
    24181 tacatgatta gtgcaatagg tttggttttg ggtattgtga attattggta tcaacgacaa
    24241 catgtggctc atatcaatac aaaagcggat cttcgtaaaa taacagcctt tcactgggga
    24301 ataataatag ctggtgtttt aatacttact ggaatatcag cttatttatt acaacatgtc
    24361 atgcttgcca aaaatctgtt gtggggtatt actctcttgg tagtgtctgt ttatatctat
    24421 ttaatgcgca aacaggctca ggcttctttt atgagaatgt tagtcgcttt ggttttaatg
    24481 attgaagcag tcatattttt tactttatac caacagatgc ctacttcatt aaatttgttc
    24541 gctgtgaata atgtaatacc tactttcctg ggcatcacta ttgatccgca aagctttcaa
    24601 gctctcaatc caatatggat tgtattaatg agtcccgtat tagcttattt ctatagaaaa
    24661 ttgcatcaac ataatatttt attttctata ccttataaat ttgcattcgg catgaccatg
    24721 tgtgcagtga gttttttaat gctgtatttt gctcgttatt tttatgttgg aaatggcatg
    24781 gtttcttcct ggtggatgat tttcagttat ttattccaaa gtcttggcga gttattagta
    24841 tcagctcttg gtgttgcaat ggttgctgaa ttggtgccgg ctgctaatgc tgggtttgtt
    24901 atggggatgt ggtttttaac ctccgcagtg gcagggttta ttggtgctac tgtcgcgtct
    24961 tttacagcac ttccagagaa catcaagcca ggggttgagt ctttattgat ttattccgat
    25021 gtatttgcct gtattggtct cgttactcta ttgattgcag ttattatgtg gttagtatca
    25081 cctcggctaa gccgctatat tacagtccct tccttggaag gggataagga tatagaaaca
    25141 gtagaatact ctacgaccta tatccccagt cactaggttt gtttatttcc cttttcggca
    25201 attgagttgg ttttaatatc accaattgcc gttttttatt tttgtcctat attgctattg
    25261 cattcttgaa tagaagataa ccagaaatta tttattacta tttttgtcct attacatatc
    25321 tcttaatgtt aatttaataa aaaatgtata aaatatgtac ttgcaatcaa tctttcccta
    25381 atcattactg ggttaaggtt ctcaggttct gtgagttaat ttgcttggag tataaagtgg
    25441 ggcttgaaaa aaaaattaag ctaagccagc gttctctgga gttattaaac gattacttaa
    25501 atttgggtgg gtctgggcct tccaatttag tgaatgggag ttcgtcgatt gatttttcat
    25561 ccttaactca actacttgcc agtaatctgt cgaaatcatc caaaacttca aaaacaataa
    25621 aaaagaaaag agaatttata gaggcagctt tgtatgccga gttgttacgt gacttaagta
    25681 cttcattgaa tggatcggat gaggaaatca agcctgcaga aaaagaacct cctgtctcta
    25741 ataagttgaa attctggctt ttggcaatag ctgggacatt attagccgca tgcgaggggt
    25801 ttgacagcat tacaacaatg atggctgtat tatcttttcc tgcctgggtt gtcctttgtg
    25861 caggtatttt attttccgct ttatctgtcg ttgtatttta tgggtttgat ttagtccagg
    25921 tttctcaaaa cctggaaata aaattaactg atactcccaa gctattggat ttatacctgt
    25981 cacaaatgga agaaattaag gcgattagaa aaaaaattga caattattct cttgcttcta
    26041 tgtcaatgga tgagttgaat gagttgcatt taatccttgt tatgctgcaa gaccgtgtca
    26101 attctttagt aaaagcaggc caaaaatttg atttggcact gaatagcccc aaaatggatg
    26161 ttgctaaaac aattgtttct ggagtatctg gaatactctt ttttggtgga ggtttttttg
    26221 caggacaatc agtagcgaca ttcatgctgg ctctggttat gacttctgtt acacctacat
    26281 tttggccagt tttattattt agtgtgattg tcggcttggc tgcctttagt ttgtactggt
    26341 atgtgcaacg cgtaggagta aataaactgg ttagcgaatg gtttggtttg gatgaggata
    26401 aaatagaaaa attatgtgga aagtccaaga tggacaaaga attgaaaaag ctaaacaacc
    26461 ttgatgaaaa ggtaatgact acttctgatt tgctctcgaa agtggctaat ttggagagta
    26521 aactaataga tatcgggaaa caaatgaagg agatgagcgc gcaccaggct cctgaaaaga
    26581 aattagagag tgctactgga ttaaaggcaa gttctaatgt gtactccttt cactttaatt
    26641 cctcaaatcg ggatcaggag agcgtttacg gtgagagggt ccttgatgat gctgtttccg
    26701 attatggcag gggtcagagt cataatgatt caatccatag cctatgttag ccaaatgcaa
    26761 aggcatatag attaacaggg agtttccttg caaataatcc ggattggctg acatataaaa
    26821 ctcttttaat tcactgctct cttcattaat tcttgagagc tgacttctta aaggaaccag
    26881 tggggaattg acaaccatag gatgattatt ggttgaaatc cagccgtttt gttttttaag
    26941 ggcgtttaga acggtcaata tttgatctgg aaccctttgt aatggtttag gatatattag
    27001 gtcactcaat tgataaataa attcgtaagt taacgggttg ttggcaaatg ctatgtaaac
    27061 taattcctcg tgaggattgg ctaacttgta ttttatcgcc tgagtgaggc cggcactttt
    27121 aatagatgcg tgaatttggt attcaggatt tagatacaaa taagcgatat aacaagtcac
    27181 ttgttttttt cccagatcaa cagattgcct gcagttacgg ctgaatccgg ctatttcttt
    27241 attttctcca tagagaaccg ttaattcccc atacaaatca ggctgtatga tgaaacgtat
    27301 aaattcttct tggctgacca ggtgataata cttggagagg aagcgatata atttatttat
    27361 taattttaag gagatttttt tcttttcttc atctacaaca taagagtgga tcgctttaac
    27421 atcgtgtctg ttcattgctc tctccatgaa acattttttt cgctttaatg gctcgctgta
    27481 taatttatcc ataaattatc taagtaatag cgaatagcag ataaaaaatt gtgcaactct
    27541 ttaaatatag actcgttttc atttttgtca aattgaagaa taacaattgt cgtcactcca
    27601 tgaaacggtc atctcagtaa ctctgtggat aggcaatata tgggaaatga taaaagaatt
    27661 gcggtggatc ctcatcgagg atcttaaata aatttataaa ggagagtcaa taaactctta
    27721 tattttcaaa atatttttaa caaaaggtat ggtgatttta cgatgagcct cccaggatgc
    27781 atcatctagc cggttgagga gctcatataa atcatgcatg tttcgagagc aacggtttaa
    27841 taaaaaatgt ccgacactct caggaagttc aaatccgcgt tttgctgcgc gcattttgag
    27901 tgtgtttatt ttttcgtcat cgtttaattc attcaactga ataactaatc cccaacttag
    27961 tctggagcgt aaatccgcaa gtttaatgtc acattttatt gggggttgat tacctgatat
    28021 aattaaaagc gaacgttcgg agtccttaat cttattatat aaatggaata aagcttcttc
    28081 ccaggccgaa tcgttagcga tggtattaat atcatcgata caaattaaag tttgatcttc
    28141 taatccttca atgctttgtg gcccccattc ttttaaaaaa gctaaaggaa ggtaaatggc
    28201 tgattgggtt aaattaatgg cttggcaaca agcttgcagt aaatgtgatt tgccgcttcc
    28261 tttagggccc caaagataga gcaacctgtc tgccttaaaa gaaagcattt caattaattg
    28321 ctgctgcaac aaaatattat taccccaatt gaagttgtcc agggttgctt catcattcaa
    28381 ttttattgcc agggccagct gtttattcat tttaacgatt ctttggggga ccaggctttt
    28441 ctgctccagg tccaaacata atcaaggtag gaagtaacgg tggtaaaggc agttaaatag
    28501 atgagtatat caactaaatc ataggggaat ctaaagtaag ctaattcaaa taaacataaa
    28561 gtgactaaag ttaactggaa gccggtattg agtttactga gtcgagaggg ctcaaaatcc
    28621 agtttgcgct gtataaagta ataccaagct aatactccca ttgaaatagt gaagtctctt
    28681 aaaaaaacaa gaagcactaa ccaccagggc aaggagttta agagagctaa agaaatgaag
    28741 ctggaggcaa ctaacaattt atctgcaaga gggtcaacaa atgagccaaa aaaactttgc
    28801 cattgaaaat gcctggctaa ccaaccatcc aaaccatcag ttaatcct
//
LOCUS       DAXLDT010000026        28777 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXLDT010000026 DAXLDT010000000
VERSION     DAXLDT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 28777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28777
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.026"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            450..1691
                     /locus_tag="ACF2SP_000915"
     CDS             450..1691
                     /locus_tag="ACF2SP_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0963 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358717.1"
                     /translation="MRYILPAGALTSLNFLRDDYQSERKVDLDQPPSLIGVPTLFGGT
                     DVIARDKQTKFIKKMIVVLRANLAKEEEITTPEQWEANLTASRVVIAACLYVQSQISS
                     PKNNSALYRLINKDLGITDTNYLDDEDKEICFLAANRVVNSSLSAFSDANAALRKANM
                     KPFTEQEWSDFSKFVSSVSVKKVSTNPYSNYPVTSITQPLFGAAFSYTGATIGYLGGD
                     VISQSSKTMSTKLQITTMVGSSLLMLGTTGPMGVALFSQVIASKLVTAFFSISLAHVL
                     GTTMGILGQGVGMGIGLPLDYAYRLIWKASATIMNYYSSYPNTSQMTGIRIADGKSIM
                     NGIVIEYTPSSDLKTEEGQQTVEITEEGKLLINGKEVTIPDGGVQLPLELINELKAQL
                     KLQEEKVSEEDSSSSTLLATI"
     gene            complement(1777..5223)
                     /gene="dnaE"
                     /locus_tag="ACF2SP_000916"
     CDS             complement(1777..5223)
                     /gene="dnaE"
                     /locus_tag="ACF2SP_000916"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFD2358718.1"
                     /translation="MRQRFVHLRVHTEFSLVDGLVQIKPLMKALPARGMSTVAVTDFC
                     NLFAAVKVFKSATEAGIKPIIGSDLPCHDLETPDNIFSLVLLCQDTAGYKNLTCLVSK
                     AYQEGQYHGQPRVQLQWIPEFSQGLIALSGGKFGDIGQALLANDDTLARKRALHWMNL
                     FPNRFYLEIQRTGRADELHYNEKLVALADELQLPLVATNDVRFINEDDFDAHEARVCI
                     HEGYTLADPRRVQQYSSQQYLRSADEMELLFSDLPSALQNTVEISKRCNVKLELGHNY
                     LPNFPIPDGTTVEAYLSHLSEIGLEERLKQLFRDKSEGELLVARGEYDKRLKIELDVI
                     NNMGFAGYFLIVADFIQWAKQNGVPVGPGRGSGAGSLVAYALKITDLDPLEYELLFER
                     FLNPERVSMPDFDIDFCMEGRDRVIEYVAEKYGRQSVSQIITFGTMAAKAVVRDVGRV
                     LGHPYGFVDKLAKLIPFELGITLSKAMEQEEELRRRYEEEEEVKELFDLALKLEGVTR
                     NAGKHAGGVVIAPSQLTDFTAIYCEEGSTQIVSQFDKDDVEAVGLVKFDFLGLRTLTI
                     IDWALATVNKQCENAGLSPIDISQIPTSDDSTFELLKACKTTAVFQLESRGMKELISR
                     LQPDCFEDIIALVALFRPGPLQSGMVDDFIDRKHGRARVDYPHPDLEPILKPTYGVIL
                     YQEQVMQIAQVLANYTLGAADLLRRAMGKKKPEEMAKQREIFTQGAVARGVDEQVATH
                     IFDLMEKFAGYGFNKSHSAAYALVAYQTAWLKAHYPAAFMAAVMSSDMDNTDKVVTFI
                     DECAHMKLQVLPPSINNSMYHFTVSDEKTIIYGLGAIKGVGESAIECIIQERNQNGPF
                     LDLFGFCQRLDLRKVNRRVIEALIKSGAFDIWKVERAVLTASLEKALKVAEKEHQNQS
                     SGQFDLFSLLEDKADEQNYVESKSWSEAQRLEGEREVLGFYLTGHPADRYRRELGDFI
                     VPVGQLNPSLHKKAIICAQVVAIRKIITKRGKKLVIIGMDDATARIDAVVFGEIFDQY
                     SDPLATGNMLVIEGEVAIDDYNGGIKVTANQLLSIASARTKFARCLELRLNQNNAAVL
                     PSIQSLLKTYSGRCAVQLAYANGVAKANLNLGQQWCVTPSDELIGLLSNLLGEEQVIM
                     RY"
     gene            complement(5298..6644)
                     /locus_tag="ACF2SP_000917"
     CDS             complement(5298..6644)
                     /locus_tag="ACF2SP_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2358719.1"
                     /translation="MTFELLWTDKCFLILLAVVLFTVLLSLRKQHIKLSFVIILQRPL
                     AISAGIVLLLFLAIGVLDSIHIKFINDKTEKTQSLLDRVLAPIGNTYEKTYSAPLALY
                     QFMSETKLVNGVTTQVYPRLKYPPASIKNEKDKSLIIKSVLMKGITYSALVILGGWLL
                     LALIKFSLGYKNIFILNSTQISAIATVAVLTFAVVIGYGLSRSFHVLGTGQIGQDIFY
                     FSLKSVRTGLIIGFLTTLFMLPLALFLGIAAGYFGGLVDDIIQYVYTTLSSIPGVLLI
                     TASVLSLQTYIANHPEQFTTLAKSADARLLALCLILGVTSWTSLCRLLRAETLKLREI
                     DYVLAARALGSSWFTIVRKHLVPNVMHIVIITLVLDFSFLVLAEAVLTYVGVGVSPMT
                     ISWGNMINGARLELARDPVVWWPMFAAFLLMFLLVLAMNLFADAVRDAFDPHQSHL"
     gene            complement(6668..7645)
                     /locus_tag="ACF2SP_000918"
     CDS             complement(6668..7645)
                     /locus_tag="ACF2SP_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2358720.1"
                     /translation="MIKYILRRILYAIPILFGINLITFALFFMVNSPDDMARMHLGNK
                     HVEQKAIEDWKAVHGYDLPLFYNEKQTGFNQFRQTLFYQKSLMLFTFNFGVSDAGRDI
                     SHDISYRMWPSLAIALPTLLFAVLVNIIFAMAMAFFRSTYLDLSGVVICIILMSISSL
                     FYIIGGQYLFGKILKWFPISGYDDGLDALKFTILPVLVAVLGQLGAGARWYRTLVLEE
                     INKDYVKTARAKGLSEQRILFKHVLKNAMLPILTGIVVIIPTLFMGSLVLESFFGVPG
                     LGSYIIDAIQQQDFAIVRAMVFLGSVLYIIGLVLTDFSYILVDPRVRLS"
     gene            complement(7642..9807)
                     /locus_tag="ACF2SP_000919"
     CDS             complement(7642..9807)
                     /locus_tag="ACF2SP_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD2358721.1"
                     /translation="MQFMIFLMPCCVNLAYGSPWVFNNPYPESEANENIYYSSFNEQP
                     KTLDPARSYSSNEYLFIAQIYEPLLEYDYYKRPYELIPLTATRLPQIRYLDAQGNPVP
                     AGDNTKPAYSVYTIQIKKGILYQPHPALAQKKDGSYRYHHLPEDYLEKHDISKLSDFK
                     YTGTRELIIDDYIYQIKRLANPAVSSPIYGLMSYYILGFKEYGDSLPKGLTNKNSFID
                     LRKYPLKGVKKLDDYTFEITLKGQYTQFLFWLAMPFFAPVPWEADLFYSQKDMDDKNL
                     SFSWYPIGTGPFMLTENNPNRSMILEKNPNFREVYFPVNGSDADRRAGYLTNQGRRLP
                     LIDKAVYTLEKESIPRWNKFLQGYYDMSGISADSFDQAIHINRFGEPILSQEMKDKKI
                     YLRQTLEPSIYYTGFNMLDSVVGGNSKRARKLRQAISIAVNFDENIAIFFNGRGIPAQ
                     GPIPPGIFGYKEGKEGINPYVYQWVNNARKRKSLKDAQKLMVEAGYPGGIDPKTGKHL
                     ILHYDVMTTGGPDDKARLDWMRKQFANIGIDLNIRATLYNRFQEKMRIGHAQIFSWGW
                     NADYPDPENFLFLLYGKNGKVKYGGENASNYENPEFDRLFNLMKNRPNDLQRQKLIDK
                     MLAIVRYDAPWVWGMHSEDFILSQDWVSNIKSNTISLNTLKYVSVNVPERNQLRRSWN
                     QPVFWPLGVLFGLLIALVIPLLITYLKKEKQPAERVKLK"
     gene            complement(9927..11948)
                     /gene="ligA"
                     /locus_tag="ACF2SP_000920"
     CDS             complement(9927..11948)
                     /gene="ligA"
                     /locus_tag="ACF2SP_000920"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFD2358722.1"
                     /translation="MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKAL
                     QQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQPMLSLSNVFTIDELKAFIKRAIE
                     KLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
                     RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEIS
                     ASRPLAIYCYSIGACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAIGVEGCLDYYQYM
                     LAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQV
                     GRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVS
                     VILEKRPINARKIELPKNCPVCGSEVVREADEAIARCIGGLYCKAQLKRMMWHFASRK
                     AMYIEGLGSVLIDQLVDEGIVHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKK
                     TTFNRFLYALGIREIGEAGARVLAEHYCDVESLKSATIEELMTLNDIGPVAASHVVHF
                     FAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSAMGREEAKAKLL
                     ALGAKVSGSVSSKTDYVIAGSEAGSKLIKATELGVAIIEEDEFLKWVNS"
     gene            12170..13069
                     /locus_tag="ACF2SP_000921"
     CDS             12170..13069
                     /locus_tag="ACF2SP_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358723.1"
                     /translation="MELLRILSTLIVLLFSTLIHSHSDTNSNQGIAFVHGTRDHREDA
                     YGGYWKIDFIQSISQTLAKPENHYVVHCDFSQYMWHEDAANCVANQLLEFINDKNVSS
                     LTVYTHSDGANIIRWILSNPTYDGRYMTLKRKISQVIAIAPSSGGTPLADEVLNGGIF
                     ETSLAWLLGYLSDAVKQQRVGDMLIYNEELLLGSKGRPSLSIPFKVIVGTDVYASPFS
                     TSSYCNGYMLNSGLKIAKLYLNQCADGFLNCDSQAQAGEVWFYDIDKTDYNLPLSHNQ
                     SRHSCFGLDQILISALVNEGVVK"
     gene            13066..14244
                     /locus_tag="ACF2SP_000922"
     CDS             13066..14244
                     /locus_tag="ACF2SP_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4785 domain-containing protein"
                     /protein_id="HFD2358724.1"
                     /translation="MKTAHLIWISAFCIAQAHAFTLPRQPTKSYECENCSQLSRENLH
                     DKWEITNEPLNNNISNVRRSYGYKERISLEQLQRGVIISTLAPGAVVRITPLQNKSIP
                     ELLIKTPKNQLLPLKEASSLYNQDDEVGNNPLAITKHQAMLQIKPELGYGKFILKSKD
                     ITNKYADAYMISVLDKFSITYLEVETDSLHYQYGDKLKATISLHNDITEYDVNDVDAR
                     LVGPKGQVISLNLTKLKSNVFEGTATLDSELNDRGENWYLETDVQTEYGQEIIRRSGH
                     TAFSYSIPSASLMNVKKLSSKPLTFVVTVDVATASRYALQSVLFQKNGKGEARPIQTS
                     QRAQWLEPGKHVLQFTFDNHNQLSDDNLYLGYLRLIDYGQLKTVYQYNQPVKLSQLVD
                     "
     gene            14248..15510
                     /locus_tag="ACF2SP_000923"
     CDS             14248..15510
                     /locus_tag="ACF2SP_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFD2358725.1"
                     /translation="MALFSSFTLFEGIALAIALQFLLLFWVLIKQAQQKKLTEQLGER
                     IQQTLISQLHQLHIDLSQSYQTSQQFINEKIAQGQLTTQQLISDAIQRQMADVREQMT
                     HSFKHHASSLTSHLQLLTEEIRNHLNTLTQQVNHKLTEGFEKTSSTFIDVVKRLTIID
                     EAQKKITELSSHVVSLQDVLVDKKARGAFGEVQLSTLISNMLPSASYQMQYTLSNQKR
                     ADCVLFLPEPTGNIVIDAKFPLETYQRLINSDALSTDRKSLQQQFRQDIQKHIKDIAE
                     KYIVPNETTDGAMMFIPAESIFAEIHANYPDLIAMSQKLKVWLVSPSTLMAVLTTARA
                     VLKDDATRKQVHIIQKHLQALANDFQRFEKRMDKLSKHIDLAHQDVNEVNTSAKKITS
                     RFQKIESVDIELDEHEVTQLELVESESI"
     gene            15587..19048
                     /gene="mfd"
                     /locus_tag="ACF2SP_000924"
     CDS             15587..19048
                     /gene="mfd"
                     /locus_tag="ACF2SP_000924"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFD2358726.1"
                     /translation="MNTKTLLYQPTKNKQIWGQLYGSSLALALAEYCQQTPGIKLLIT
                     QDNLCASQLQDELQFFLNPNQNTQELLFFPDWETLPYDQFSPHQDIISERLYTLSRVQ
                     QITNGIIISSASTLMHRLSPPEFLNQYALMLKEGQKLDLNSFRNQLQQAGYHCVNKVL
                     EHGEYALRGSIIDVYPMGSGLPFRIELFDDEIESLREFDPETQRTIEKINQIHLLPAR
                     EFPLNEQSQLHFRRAFRESFPGNPSHCPIYEAISNSQFPSGIEYYLPLFFDKTVTFFD
                     YLPANAKICLIEETQENAERFWRELNERYEQRRHDVSRPILTPDNCFINPTELLTKAN
                     NYEQVRLYHKPSEKKGALNFNIAKAPQLPIDRKNIEPLRQLSEYCSDPTRRYLIVVES
                     AGRREVLLDLLKSSHIIPRTQNSWLDFIYDESPINITTGPLIYGCELTDKQIVIIVES
                     QLFGEQSTPQRRSTQKSVDPDLIIRDMAELRLGAPVVHLQFGVGRYQGLQYIDSNGTP
                     SEFLVLAYAGDDKIYVPVTSLHMISRYTGVDSEHAPLHKLGSDQWQKEKKKAAEKIHD
                     VAIELLDLYAKREAQPGHQYDFNQSEYIKFASGFPFTETPDQLQAIEQIIRDMQSPRP
                     MDRLICGDVGFGKTEVAMRAAFVAVQNGKQVCILAPTTLLAGQHFESFRDRFAEFPVN
                     IELLSRFRSNKESEAVLAALQSGKVDIVIGTHKLFQNNISFKNLGLLIIDEEHRFGVK
                     QKEHIKSLRTHVDILSMTATPIPRTLNMAMAGIRDISLIATPPAKRLAIKTFWQEKND
                     LTIREAILREILRGGQVFYLHNNVQTIERVCQDLEALVPEAKIQSAHGQMRERQLERI
                     MSDFYHHRFNVLVCTTIIETGIDIPTANTIIIDRADKFGLAQLHQLRGRVGRSHHQAY
                     AYLLTPNEKLLTPDAVKRLEAIVSLEDLGAGFTLATHDLEIRGAGELLGEEQSGNMHA
                     IGFNLFMEMLDRAVNDLKAGKTPELSAPMHQGPEIDLRISAIIPEDYIGDIHNRLIMY
                     KRIANAKTTQQLRELQIELIDRFGLLPQPVKHLFLITELKLKAAQLGIQKISASAQQG
                     KLDFSEKPSIDPGTLISLIQVHAKRYQMEGPQRLRFTLDSTSAEERIFEISSLLNKLS
                     PHSQTID"
     gene            complement(19027..20694)
                     /locus_tag="ACF2SP_000925"
     CDS             complement(19027..20694)
                     /locus_tag="ACF2SP_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFD2358727.1"
                     /translation="MQKESNSNSLFNLLLKNEKNSADHVYLRQPKSGVWHEYTWSAVM
                     LQARKVAGFLHHLGLKKGDHISIISKNCAEWFIADFGIHIAGMVNVPLFANQNEESAH
                     YVLDHGDVKLVFVGKLDDHQRVRQFIPKSYPTIGFDYHKDLQVDYEWSDVLKSDPMIN
                     LVEPEPDALYTIIYSSGTSGAPKGAMYTNRSIANYLTVFPKDLLRIRELEHYKLISYL
                     PLAHVYERSAIQLGSVAINASVSFVESLEKFAENLREVSPTFFTAVPRIWGVFKQKIE
                     QKLPPAKLNFLLKIPLISSLIKRKIIHSLGLSECTNCFSGASHLPISIIDFFEKLGIY
                     IQEGYGQSENLAYATLSMLNERKRGYVGTPRLEVEIKIGEENELLMKCPCLMSGYYKN
                     EQATKNAFTEEGWLRTGDVVDVDNEDRVKILGRLSENFKNQTGEFIAPSPIEKLFSVN
                     SMLEQLCLVGRELPNNVLLVTLNEGVRLGMPKEEITHSLQETLKQVNSKLVKYEKVSH
                     ILVLKDVWTTENDKLTPTLKVKRRVVEKDYAELIQKALQQSQSIVWE"
     gene            complement(20721..21599)
                     /gene="rssA"
                     /locus_tag="ACF2SP_000926"
     CDS             complement(20721..21599)
                     /gene="rssA"
                     /locus_tag="ACF2SP_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFD2358728.1"
                     /translation="MTNQYPKIGLALGSGSARGWAHIGVIQSIKRLGVRIDCVAGCSI
                     GALVGAIYACGTLDLFEQWVLKLNKREMAKLIDVNFLGGGVISGSKLMTFFKDFGLEK
                     NIEELSLPFSSVATDLSTGREIWLSKGSLELAIRSSMSLPGLFTPQRYKDCWLVDGGL
                     VNPVPVTLCRAMGADVVIAVNLNHDLLRVPPSTTEHPGFFSVLSGQFDKMIKKIMGIS
                     DGPGYYDVLTRSIKIMQDRITRARAAGDPADLTLVPQLGNIMLYDFHKAKEAIHEGEN
                     CVLREKNNILQLVGLE"
     gene            complement(21645..23087)
                     /gene="tldD"
                     /locus_tag="ACF2SP_000927"
     CDS             complement(21645..23087)
                     /gene="tldD"
                     /locus_tag="ACF2SP_000927"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFD2358729.1"
                     /translation="MTKSLAIAKNILLTPASLDEVGIEKLFKTMFNRNVDDADLYFQS
                     CSYESWYLEDSEVKSGNFSLDKGVGIRAVSGDKTGFAYCDDILLPSMLRAADAARSIA
                     LSGSKTVPAVKVLKASVNRYESLNPLEGMSKQEKIALLEAIDKEARSIDPRVIQVNAT
                     LTGCYEVVMVANMDGQMMADVRPLVSINVSVIVEDSHGRRESARSGGGGRVAYSYFTE
                     KENALSYAREAVREALTNLKAEPAPAGTMPVVLGPGWPGVLLHEAVGHGLEGDFNRKG
                     LSAFSGRLGQQVAAAGVTVVDDGTLENRRGSLTIDDEGTPSQCTTLIDNGILVNYMQD
                     KLNAKLMGMQSTGNCRRESYAHVPMPRMTNTYMLSGQYDPQEIIRTVKKGLYAVNFGG
                     GQVDITSGQFVFSASEAYLIENGKITKPVKGATLIGNGPDVMKKISMIGNDLALDRGI
                     GVCGKEGQSVPVGVGQPTLKIDALTVGGTK"
     gene            complement(23099..23905)
                     /locus_tag="ACF2SP_000928"
     CDS             complement(23099..23905)
                     /locus_tag="ACF2SP_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFD2358730.1"
                     /translation="MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFA
                     FMGMNEREKLHIAEYYGQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYD
                     DKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
                     ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRH
                     TYGHSMVVEPWGKVLAQKEEGQGVILADIDLERLSQLRRQFPCVEHHVLM"
     gene            24124..24798
                     /locus_tag="ACF2SP_000929"
     CDS             24124..24798
                     /locus_tag="ACF2SP_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFD2358731.1"
                     /translation="MNRNDITILSQQRESVLATNKVLRNTYLLLSLTFIFSALTAYVA
                     FISGARPMNPLLMIVGVYGLMFLTQALRNSVWGLVSVFAFTGFLGYTIGPLLNYYITG
                     FSNGPQIVATALGGTGMIFFALSGYALTTKKDFSYLGGFLFVGVMVALLAMIAGIFIQ
                     IPALQLVISAAFVLISSGLILLQTSAIIHEGETNYIMATIGLFVSIYNLFVSLLNLLS
                     AFSGRD"
     gene            complement(25186..26154)
                     /locus_tag="ACF2SP_000930"
     CDS             complement(25186..26154)
                     /locus_tag="ACF2SP_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HFD2358732.1"
                     /translation="MNSNYKREDFANETEVRWCPGCGDYAILAALQRVLPELGLPPEQ
                     HVFVSGIGCAGRLPYYMNTYGFHTIHGRATAVATGLKAMREDLCVWVITGDGDALSIG
                     GNHLLHILRRNVNVNILLFNNQVYGLTKGQFSPTSQKGQVTKTSPKGVTSEPINPIMI
                     ALASGASFVARAVDKDPVHLASVLKKAYEHKGCSFVEIYQDCNIFNHGAFDDFAVKNN
                     RAENTIILEDGQPLVFGAQKEKALQLDDEQFVQISIDEKTLYKHDEKNLISAMRLARL
                     TFPDYPVPLGVYYQKERELFTLSQEVKKRLEDLPELFKAKASWKQN"
     gene            complement(26138..27955)
                     /locus_tag="ACF2SP_000931"
     CDS             complement(26138..27955)
                     /locus_tag="ACF2SP_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HFD2358733.1"
                     /translation="MSTTDVIVVRITGDSGDGVQLVGEQLTISAALTGRDVRTMPDFP
                     AEIRAPAGTVAGVSGFQLAMAERAIFTAGESLDVLVALNPAALKNSLQHLNQGGLLII
                     NEDSFQERDWQKAGIDKSFLDNCREHYHIVAFPLITQTIQAVESINLTKPQAVKTKNF
                     YILGLVLWLFDLPTETCQAFIAKKFKTNQAIAKANELALLAGYNYAMTLELSRRDYML
                     GEVNRHSGEYRQITGVEAVGLALATLATQTQVPLLVSGYPITPASAILHECARLSDYG
                     VQLIQAEDEIAAICSCIGAAYGGRLALTCTSGPGLDLKCESLGLAVVTELPLVLVDVQ
                     RAGASTGLPTKTSQSDLKQALYGRHGEAPLPVIAAQSPADCFNTIIEAFRIAIKYMTP
                     VIVLLDAYLANAAEPWEIPALDSLNIPKPEFNRFTKPYHRDEFLSRSWNVPGTSGFIH
                     QIGGLEKQGDEGRVSYDAENHQKMVITRAEKIKGIARDYPALVLEGDMTAPTLVIGWG
                     STFGSLKSAVQQCLEQGLPVAYLHLRHLNPLPDDLGSLLKKYDKVLVAELNSGQLCQL
                     IRAVYLVDAQSISQCNGQPFSVNHLVSAIKAEANYEQQL"
     gene            complement(27942..28688)
                     /gene="pdxJ"
                     /locus_tag="ACF2SP_000932"
     CDS             complement(27942..28688)
                     /gene="pdxJ"
                     /locus_tag="ACF2SP_000932"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFD2358734.1"
                     /translation="MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGIT
                     LHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAMLDFAEEILPEHACLVPEKREEL
                     TTEGGLDILTHRNVVERAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
                     ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHA
                     IIARALFCGLKEAVRHMRQLMQEARLYVND"
BASE COUNT         8929 a         6464 c         5014 g         8370 t
ORIGIN      
        1 aataaaaccg agcataacaa aaattgcttt actccagtgc gatagacgaa gaagtaaaag
       61 atattggtat aacttatcca tgtaacttac cctgatgatt ttatttgcta attccatcat
      121 acaaatctac ccttttctca aaaacagcta aaacagttct gtcatttcta atcaaagtat
      181 taatgaccgc ctcaatcagg ctacaggaca tggatttgtt ttaattttaa attaattaca
      241 ccctataaga gtaacatatc taactcaagg ctaaaaatag tataatcgca aaaaaatcat
      301 catttcaaag agttaaatca taaataaaat ctgtagcctt atctgattgc aacaatttag
      361 taaaaattaa ccaaatcaga ttgcatttaa ccagctaatc ggtaagataa aaaacacaca
      421 aataaatttt caatggatat gaggttatta tgcgctatat actgccagca ggagctctaa
      481 ccagccttaa ctttttacgc gatgactatc aatcagaaag aaaagtggat ttggatcaac
      541 ctccatcttt aatcggagta cctactcttt ttggaggcac tgatgtcata gccagagata
      601 aacaaaccaa atttataaaa aaaatgattg ttgtccttag agcaaatctg gcgaaagagg
      661 aagaaatcac aacccctgaa caatgggaag cgaatttaac tgcttctaga gtcgtcatcg
      721 cagcctgtct atatgttcaa tcgcaaattt catctcccaa aaataactcc gcattatatc
      781 gattgattaa taaggattta ggcattactg atacaaatta tctggatgat gaagacaagg
      841 aaatctgctt tttagctgcc aatcgagtgg tcaactcctc tttatccgcg tttagcgatg
      901 ccaacgctgc cttgcgcaaa gccaatatga agccatttac cgagcaggag tggagtgact
      961 tttccaagtt tgtcagtagt gtgagtgtta aaaaggtatc taccaatcct tactcgaact
     1021 atcccgtcac atccattact caaccgttat tcggagccgc tttttcttat acaggagcaa
     1081 cgattggtta tcttgggggg gatgttatta gccaatcctc caaaacgatg tcgaccaaac
     1141 tacaaattac taccatggtg ggaagcagcc tgctaatgct tggtacaaca ggacctatgg
     1201 gcgtggcgtt gttctctcag gttatagcga gtaaattagt cacggcattt ttcagtatta
     1261 gtttggctca tgttttagga acaaccatgg gtattctcgg acaaggcgta ggaatgggca
     1321 tcgggttacc tttggattat gcctacagat taatatggaa agccagcgcc actattatga
     1381 attactacag cagctatcct aatacctctc aaatgacggg tattcgtatt gcggatggca
     1441 aatccatcat gaatggcata gtgatcgaat acactccaag ctctgattta aaaactgaag
     1501 aaggacagca aacagttgaa attactgaag aaggaaaact tcttatcaat ggaaaagaag
     1561 tcactattcc tgacggcgga gttcaacttc cactagagtt aattaatgaa ttaaaagctc
     1621 aattgaagct gcaggaagaa aaagttagcg aagaagactc ttcctcttcc acattgctag
     1681 caactatttg atgtagatga gacaactatc attctgcaat gcgagttgag ccgtgctatc
     1741 tgctcaactc cacaggcgct atcattagca gtatttctaa taacgcatta ttacttgttc
     1801 ttcccctaaa agattgctca atagtccaat taactcatcg ctaggggtca cacaccattg
     1861 ttggccaaga tttaaattag ctttagcaac accattagca taagccaatt gaacagcaca
     1921 ccgtcctgaa taagtcttca gtaaggattg gatagaaggc aatacagcag cattgttttg
     1981 atttaaacgc aactccagac aacgagcgaa ttttgttcga gctgaagcga tacttaaaag
     2041 ctgattggcg gtcactttaa taccaccgtt gtaatcatca attgcaacct caccctcaat
     2101 tacaagcatg ttacccgtcg ccaacggatc agaatattga tcaaaaatct caccaaaaac
     2161 tacggcatca atcctggcag tcgcatcatc cattccaata atcaccaatt tcttgccacg
     2221 tttggtgata atttttctaa ttgctacaac ctgagcgcaa ataatcgctt ttttatgtaa
     2281 agacggattt aattggccga caggaacaat aaaatctccc aattcacgtc tgtatcggtc
     2341 agcaggatgg ccagtaaggt aaaaacctaa tacctctctt tcgccttcta atctttgggc
     2401 ttcagaccag gatttacttt ccacataatt ttgttcatcc gctttgtcct ctaaaaggga
     2461 gaatagatca aattgtccac ttgattgatt ttgatgttcc ttttcggcaa ctttcagtgc
     2521 tttttccaag gacgctgtta atacagccct ttccactttc caaatatcaa aagcaccact
     2581 cttgattaaa gcttcaatca ctcttctgtt gactttgcgt aaatccaatc tttgacaaaa
     2641 accaaacaaa tccaaaaaag gtccattctg gtttctctcc tgaataatac actcaatcgc
     2701 agattcgcca acccccttta ttgccccaag gccataaata atcgtctttt catcggatac
     2761 ggtaaaatga tacatggagt tattgataga aggaggcaac acttgtaatt tcatgtgcgc
     2821 gcattcatca ataaaagtta caaccttatc cgtgttatcc atatcagagg acatcaccgc
     2881 agccataaaa gctgcaggat aatgtgcttt tagccaggcg gtttgataag cgactaaagc
     2941 gtacgcggca gaatgtgatt tattgaatcc atatccggcg aacttctcca ttaaatcaaa
     3001 aatatgggtt gctacctgtt cgtctactcc tctcgcaaca gctccctggg taaaaatttc
     3061 cctttgcttt gccatttctt ccggtttttt cttccccatg gctcgtctta acaagtcagc
     3121 agcgccaagc gtgtaattgg caagaacctg agcgatttgc attacttgtt cttgatagag
     3181 gataacccca taagtgggtt taagaattgg ttccaagtct ggatgggggt aatcaactcg
     3241 agccctgcca tgcttcctgt cgataaagtc atccaccatc cctgactgta gaggacctgg
     3301 tctgaataaa gcaaccaaag caatgatgtc ttcaaaacaa tcgggttgaa gccggctaat
     3361 cagctccttc atgcctcgag attccagctg aaatacagca gtagttttac atgcttttaa
     3421 taactcaaaa gtagagtcat cactcgttgg aatttggcta atatcaattg gtgacaaccc
     3481 ggcattttcg cattgcttat tcactgtggc cagggcccaa tcgattatgg ttagtgttct
     3541 taatcctaaa aaatcaaact tgactaaccc aaccgcctca acatcgtctt tatcaaattg
     3601 gctaactatt tgtgttgatc cttcttcaca atatattgcc gtgaaatcag tcagctgaga
     3661 aggtgcaata accactcccc cagcatgctt gcctgcatta cgagtaactc cctccaattt
     3721 cagggcaaga tcaaatagct cttttacttc ctcctcttct tcatatcgtc ggcgaagctc
     3781 ttcttcctgc tccatcgctt tacttaaagt tatgcctaac tcaaagggaa ttaatttcgc
     3841 aagcttatcc acaaagccat aaggatgacc taaaacacgc ccaacatctc tcaccacagc
     3901 ctttgcggcc atagtgccaa aagtaataat ttgggaaacg ctctgccggc catatttctc
     3961 tgctacatat tcaatgaccc gatcacggcc ttccatgcaa aaatcaatat caaagtcagg
     4021 catagaaaca cgttcggggt ttaagaatcg ttcaaaaagc agctcatact ctaaaggatc
     4081 cagatctgtt atttttaggg cataagcaac cagagaacca gcaccagaac cacgacctgg
     4141 tcctactgga accccatttt gtttagccca ttgaataaaa tcagcaacaa tcaggaaata
     4201 gccagcaaag cccatgttat taataacatc cagttcaatt ttaaggcgtt tgtcgtactc
     4261 tcctctggca acaagcagtt ccccctcaga cttatctctg aataattgct ttaaacgttc
     4321 ctccaagcca atttcagaaa gatgtgataa atacgcctct actgttgttc catccggtat
     4381 agggaaatta ggtagatagt tatggcccag ctcgagtttt acattacagc gtttactaat
     4441 ttcgactgtg ttttgtaatg cactgggaag atccgaaaat aagagctcca tttcatctgc
     4501 tgagcgcaga tactgttggg agctgtattg ttgaactctc ctgggatcag ccaaagtgta
     4561 cccttcgtga atgcatacac gagcttcatg agcgtcaaaa tcatcctcat tgatgaacct
     4621 tacatcgttg gttgcaacta ggggtaattg caactcatct gctaacgcga ccagcttctc
     4681 attataatgc agctcatcag ctcttccggt tcgctgaatt tccagataaa accgattcgg
     4741 aaataaattc atccaatgca aagcacgttt tcttgccaag gtgtcatcat ttgccagcaa
     4801 tgcttgccca atgtcaccga actttcctcc agataatgcg attaatccct gtgaaaattc
     4861 tggaatccat tgtaattgca ccctgggttg gccatgatat tgcccttctt gatatgcttt
     4921 ggataccaga caggttaaat ttttataacc tgctgtgtct tgacataata aaaccaaaga
     4981 aaaaatgtta tccggggttt ccaaatcatg gcatggcaaa tcactaccaa taatgggttt
     5041 gattcccgcc tctgtcgcac ttttaaatac tttgacagca gcgaataaat tacaaaaatc
     5101 cgttacagca actgtgctca tacctctggc tggcaaagct ttcatcaatg gcttaatctg
     5161 taccaaacca tcaactaatg aaaattccgt atgtactctt aaatgaacaa atcgttgtcg
     5221 catagagaat taacccattc acgataattt cgaaacttta acctaaatcc cggaaatagg
     5281 aaactaacag atattgacta taaatgagat tgatgtgggt caaaagcatc ccgtactgcg
     5341 tctgcaaaca agttcattgc caaaaccaat aaaaacatta acagaaaggc tgcaaacata
     5401 ggccaccata caacagggtc acgtgctaat tcaaggcgcg ccccattaat catatttccc
     5461 caactaatag tcatgggtga aacccctacc cctacatagg tcaatacagc ttctgccaag
     5521 actaaaaaac tgaaatccaa taccagggtg atgataacaa tatgcatgac attgggcacc
     5581 aaatgtttgc gtacgatggt aaaccaacta ctgcctaagg cacgtgcggc caggacataa
     5641 tctatttccc gcaattttaa agtttctgct cgcaaaagac ggcacaggct tgtccaactg
     5701 gtcacaccca gtatcaagca aagagctaat aaacgtgcat cggcactctt ggccaaagta
     5761 gtaaattgct ccgggtggtt tgcaatataa gtttgcaatg acaatacgga agccgtaatt
     5821 agcaaaacac ccggaatgga gcttaaagta gtataaacat actggataat atcatccaca
     5881 agcccaccaa aataccctgc tgcaatcccc agaaataaag ccagtggcaa catgaagagg
     5941 gttgttaaaa aaccaataat tagacctgtt cgcacacttt tcaagctgaa atagaaaata
     6001 tcttgtccaa tttgccctgt gcccaataca tgaaaggatc ttgataatcc gtaaccaatg
     6061 acgaccgcaa aagtaagcac agctactgtc gctatagcgc tgatttgagt agaatttaaa
     6121 atgaaaatat ttttatatcc aagactaaac tttattaaag cgagtaataa ccacccaccc
     6181 aaaatgacta aagcactata tgttatacct ttcataagca cagacttgat gataagggat
     6241 ttgtcctttt cattcttaat agaagctggt ggatatttta agcgaggata aacctgagtt
     6301 gtgactccat taaccaactt cgtttcactc ataaattgat ataaagccaa aggagctgaa
     6361 taagttttct cataggtatt ccctatagga gcaaggactc tgtccaatag agactgcgtt
     6421 ttttctgttt tatcattgat gaatttaata tgaatggagt ccaaaacacc gatggctaaa
     6481 aacagcaaca acacaattcc agcactgatt gctaagggcc tttgtaaaat gattacaaat
     6541 gacaatttga tgtgttgttt tctaaggctc agcaaaacgg taaataaaac caccgccagt
     6601 aaaataagaa aacatttgtc agtccaaagc aattcaaatg tcatttttgc cacacactcc
     6661 ttggttatca ggataatcgc actcggggat caactaggat gtaagaaaaa tcagtcagaa
     6721 ccaagcctat gatgtaaaga actgacccca gaaataccat agctcgtaca atagcgaaat
     6781 cttgctgttg tatcgcatca ataatatagc tacccaagcc tggcacacca aaaaaagact
     6841 ctaaaaccaa actccccata aacaaagtag gaatgattac aacaattccg gtcaaaatgg
     6901 gtaacatggc attttttaat acatgcttaa ataaaatcct ttgctcagag agaccctttg
     6961 ctcttgcagt ttttacataa tctttattaa tttcttccaa aaccaaggtt cgataccaac
     7021 gcgctcccgc ccccaattgt cccagaacag ctaccaggac tggtaaaata gtaaatttta
     7081 aagcatcgag gccatcgtcg tagccggaaa tagggaacca ttttaatatt tttccaaaca
     7141 gatattgccc accaatgatg taaaataaac tggaaatgga cattaggata atacaaataa
     7201 ctaccccgga taaatcaaga taagtcgaac ggaaaaatgc catagccata gcaaaaatga
     7261 tattcaccaa aacagcaaat aataatgttg gcaaggcaat agcaaggctt ggccacattc
     7321 tgtaggatat gtcgtggcta atatctctac cagcatccga gacaccaaaa ttaaatgtaa
     7381 acagcatcag cgatttttga taaaacaaag tctgtctaaa ttgattaaat ccagtctgct
     7441 tttcattata aaataacggt aaatcatagc catgaaccgc tttccaatct tcaatggctt
     7501 tttgctctac atgtttatta cccaagtgca tacgtgccat atcatcggga gaattaacca
     7561 tgaaaaacaa tgcaaaagta ataagattaa taccaaacaa aattggaatc gcatataaaa
     7621 ttctgcggag tatgtattta atcattttaa ttttactctt tctgccggct gtttttcttt
     7681 ctttaaataa gtaatcaata aagggattac cagagcaatc aataggccaa acagcacacc
     7741 taaaggccaa aataccggtt gattccagga tcttcgcagc tggttacgtt caggtacatt
     7801 aacagaaaca tattttagag tattgagaga aatcgtattg gattttatat tagataccca
     7861 atcttgcgat aaaataaagt cttcagaatg catgccccat acccatggag catcataccg
     7921 gacaatcgct aacatcttat caatcagttt ttgtctttgt agatcgttag ggcgattttt
     7981 catcaaatta aacaatctat caaattcagg attttcataa tttgatgcat tttcaccacc
     8041 atacttcact tttccatttt tcccataaag caaaaataaa aaattttcgg gatcaggata
     8101 atcggcattc cagccccaac taaatatttg agcatgacct atccgcatct tttcctggaa
     8161 acgattgtaa agagtcgccc taatattcaa atctatccct atatttgcga attgctttcg
     8221 catccagtca agccttgctt tgtcatcagg tccacctgtc gtcatgacat cataatgaag
     8281 aatcaggtgt ttcccggttt tcggatctat cccacccgga tatcctgcct caaccattaa
     8341 cttctgcgca tcctttaagg attttcgctt cctcgcatta ttcacccatt gataaacata
     8401 gggattaata ccctcttttc cttccttgta cccaaagata ccaggtggaa tagggccttg
     8461 cgccggaata cctcggccat taaaaaatat ggcaatattt tcatcaaaat tcaccgcaat
     8521 agaaattgcc tggcgtaatt ttcgtgctct tttgctattg cccccaacaa cgctgtcaag
     8581 catattaaag ccagtataat agatagacgg ttccagggtt tgtcttaaat atattttttt
     8641 atctttcatt tcctggctaa ggataggctc tccaaagcga ttaatatgga tagcctgatc
     8701 aaaactgtca gcacttatcc ccgacatatc atagtagcct tgcaagaatt tattccatcg
     8761 aggtatggat tccttttcaa gggtataaac ggctttatca attaagggca agcgccttcc
     8821 ctggttggtt aaataacctg cccgtctatc ggcatctgaa ccattgacag gaaaatacac
     8881 ttctctgaaa ttaggatttt tctccaatat catgcttcta ttgggattat tttcagtcag
     8941 cataaaaggc cctgtaccta taggatacca gctaaaagat aagttcttat catccatatc
     9001 tttttgggaa taaaataaat ccgcttccca tggaaccgga gcaaaaaagg gcatggctaa
     9061 ccaaaacaaa aattgtgtat attgtccctt taatgtaatc tcaaaggtgt aatcatctaa
     9121 ttttttaacc cccttcaaag gatacttacg taaatcaata aaagaatttt tattggtcaa
     9181 tcctttagga agactatctc catactcctt gaagcccaga atataatagc tcatcaatcc
     9241 atatatcgga gaactcacag cagggtttgc caatctttta atttgataaa tgtaatcatc
     9301 aatgatcagc tcccttgtac cggtgtattt aaaatctgac aacttgctaa tatcatgctt
     9361 ttccaaataa tcttctggta agtgatgata tcgataagag ccatctttct tctgagccaa
     9421 agcaggatga ggctgatata aaatgccttt ttttatctga atggtataga cactgtatgc
     9481 tggctttgtg ttatctcctg ccggaacagg atttccctgg gcatccaaat atcgaatctg
     9541 tggtaagcgg gtagctgtta atgggattaa ttcatagggt cttttatagt aatcatattc
     9601 cagcagtggc tcatagatct gggctatgaa aagatattca ttggaagaat aggaacgtgc
     9661 cggatccaga gtttttggct gttcattaaa agaagaataa taaatatttt cattggcctc
     9721 gctctctggg taaggattat tgaataccca aggactgcca taagccaaat tgacacagca
     9781 aggcattaaa aatatcatga attgcattaa caaatatcgc cgcacttact tccctcgtgc
     9841 caatcagtca ttattacagg tttaaatacc tacttcaccc gattcattga tacccatcat
     9901 aaaccaaata acaaagatct agctactcat gaattaaccc attttaaaaa ctcgtcttcc
     9961 tctataatcg ctactcccag ttctgtcgct ttaatcagct ttgaaccggc ttcacttcct
    10021 gctattacat aatccgtttt ggaagacaca cttccactca cttttgcacc taaggctaat
    10081 aattttgcct ttgcctcttc cctccccatg gcactcagag ttccagttaa aactactgtt
    10141 ttaccaaaaa atggattttg ctgattaacc tgtatttttt cgggcttagg ccaatgaata
    10201 cccaactcga gaagacggtc aatcacttca agattatgcg cttgagcaaa gaaatggact
    10261 acatgagaag ccgctactgg acctatgtca ttcagagtca ttaattcctc aatcgttgct
    10321 gatttcaagc tctctacatc acagtagtgc tccgctaaaa cccttgcgcc agcttcaccg
    10381 atttctctga tccccaaagc ataaagaaag cgattgaaag tcgttttttt acttttttcc
    10441 aaagcggata ataagttttt tgcagatttc tcccccatcc ttggcaggtt agctaaagtc
    10501 tgcaaatcga gttcataaag atccgccaaa tgatggacaa taccctcatc aactaactga
    10561 tcaattaaaa cactaccaag tccttcaata tacatcgctt ttcgagaagc aaaatgccac
    10621 atcatccttt ttaattgtgc tttacaatat aaaccgccga tacaccgagc aattgcttca
    10681 tccgcttccc ttacgacttc agaaccacaa acagggcaat ttttaggaag ctcaatcttt
    10741 ctggcattaa tgggacgctt ttccaaaatc acagaaacta cttcagggat cacatcaccg
    10801 gcacgacgaa taataaccgt atcaccaata cgaatgtctt ttcgtgtaat ttcatcaaaa
    10861 ttatgcaaag tcgcgttact tactgtaaca ccaccaacac taactggctc caaacgtgca
    10921 accggcgtca cagcacccgt tctacctacc tggaaatcca cggctatcag acgagtcatt
    10981 tcttctgtag cgggaaattt atgggcacaa gcaaatcttg gggcacgaga aacataacct
    11041 aattgctgtt gcaaggagat actgtcaatc ttataaacaa ccccatcgat ttcaaatggc
    11101 aattgattcc gtttcgctaa catatactgg taataatcta aacagccttc tactccaatc
    11161 gccctcctcg tttctggaga cactctaaat ccaaactctt ttaataaatt caattgctcc
    11221 aaatgactgt taggtaactt ataatcctcg caagccccta tactataaca ataaatagca
    11281 agtggacgac tggcagaaat ttcaggattt aattgtctta aactgcctgc agcagcattt
    11341 cgcggattag caaaagtttt ttcaccgagt tctctagccc ttgcgttgta agcttcaaaa
    11401 tcggctttgg ggatatagac ttcaccccgc acttcgatca attttggagg gttactaacc
    11461 cttagcctta atggaactga agcaatagtc ttaatatttg ccgtgatgtt ttctcctaca
    11521 gcaccatcgc cacgagttgc ggcatgagtc aagatcccgc cctcataagt catgttaaca
    11581 gccaacccat caagctttgg ttcgcaagca aataccagtt gttgatttgg ttcatccagt
    11641 ttctcaattg ctcgtttaat gaatgctttt aattcatcga tagtaaacac attggataaa
    11701 gacaacatgg gttgcttatg ggctaccggc ataaaagcat ctgaaggagt accgctcact
    11761 ctctgtgtag gggaatctgg cgataaaaat tgcggatatt gctcttcata ctgttgcaat
    11821 gccttgaaac atcgatcata ttccgcgtca ggaaccaaag gttcatctaa aacataatag
    11881 tgataatcgt attttcttat ttgctcttta agcgtttcta tcgattcctt aattccttga
    11941 tcattcatta atgaagattc ccaaaataaa aacaacagtt tatcattttt ttacttcttt
    12001 caaatatgag tggtttaatc attcaaggca aaatcttttt ttggaagcct ctttattcgg
    12061 aaccaagtaa tcagctcaaa tccactaaaa cgcatcaatc ccatctcttg aatcttttgc
    12121 cagaaccatg atatagatac gtctctgtat tgaaggagat attttgaaaa tggaacttct
    12181 gcgcatttta tccactctta ttgttttatt gttttcaacc cttatccatt ctcattccga
    12241 cactaattcc aatcaaggca ttgcttttgt ccatggtact cgagatcaca gagaagatgc
    12301 ctatggaggg tattggaaaa ttgatttcat acaaagtata tcccaaaccc tggcaaaacc
    12361 tgaaaatcac tacgtagttc attgtgactt tagccaatat atgtggcatg aagacgcggc
    12421 caattgtgtt gccaatcaac ttctggaatt tattaacgat aaaaatgtga gctctctcac
    12481 agtctacacc cactctgatg gtgccaacat catacgttgg attctctcaa acccaactta
    12541 tgatggccga tatatgactc tcaaacgcaa aatcagtcaa gtgattgcca ttgcaccttc
    12601 cagcggaggg actcctcttg ctgatgaagt attaaatggc ggcatatttg aaacgagtct
    12661 ggcctggtta ctcggttatc tttcagatgc agtcaaacaa caacgtgtag gcgacatgct
    12721 gatctataac gaagaactgc tattgggaag caaaggccga ccctctctgt ccattccatt
    12781 taaagtcata gtgggtactg atgtctatgc ttcaccattt tcaacatcca gctattgtaa
    12841 tggatacatg ctaaatagcg ggctaaaaat tgccaaactc tatcttaatc agtgtgctga
    12901 tggctttctg aattgtgatt cgcaagctca agcaggagaa gtatggttct atgatataga
    12961 caaaactgat tacaatttgc ctttaagcca taatcaaagt cgacattctt gttttggttt
    13021 ggatcaaata cttatcagtg ccctagttaa tgaaggagtt gtaaaatgaa aactgcccac
    13081 cttatatgga tatccgcatt ttgtattgcc caagctcatg cgtttacatt accaagacag
    13141 ccaaccaaat cttatgaatg tgagaattgc agccaattat cacgagaaaa tctgcacgat
    13201 aaatgggaaa ttaccaatga accattaaac aacaacatca gtaatgttcg tagaagttat
    13261 ggttacaaag aacgtatttc tctggaacaa ttacaacgcg gagttattat atcaacattg
    13321 gctcctggcg ctgtcgttcg cattacccct ttgcagaata aatccatccc ggaattactt
    13381 atcaaaacac caaaaaatca actcttgccc ctgaaagaag cctcttctct ctataatcag
    13441 gatgacgagg ttggtaataa tcctttagcc attacaaaac accaggctat gttacaaata
    13501 aaacctgaac tggggtatgg aaaatttata ctaaaaagca aggacattac taacaaatat
    13561 gccgatgctt atatgatcag tgtccttgat aaattttcca tcacttacct ggaggttgaa
    13621 accgattctc tccattacca gtatggcgat aagctaaaag ccaccatatc gcttcacaat
    13681 gatatcactg agtatgacgt caatgacgtt gatgctcgac ttgtaggccc caaaggtcaa
    13741 gtcatttcgc ttaatctgac caagttaaaa tccaatgtat ttgaaggaac tgccactttg
    13801 gattccgagt taaatgatcg cggcgaaaac tggtacttag aaaccgatgt tcaaacagaa
    13861 tacgggcaag aaatcattag acgatctggc catacagcct tttcatactc cattccctca
    13921 gccagtctta tgaatgtaaa aaaattgtcc tctaaacctt tgacttttgt tgtcactgta
    13981 gatgttgcaa ctgcaagccg atacgcttta caaagtgtgt tgttccaaaa aaatggaaag
    14041 ggtgaagcca ggcctattca gacctcgcaa agagcacagt ggcttgaacc cggtaagcat
    14101 gttttgcaat ttacctttga caatcacaat caattatcgg acgataacct ctatttgggt
    14161 tatttacgtt tgatagacta tgggcagtta aaaactgtgt atcaatacaa ccaacctgtc
    14221 aagcttagtc agttagtgga ttaatgaatg gcattatttt cttctttcac tttgtttgag
    14281 ggcatagcgt tggctattgc ccttcaattc ttattgcttt tctgggtttt gataaaacaa
    14341 gcacagcaaa aaaaactgac tgaacaactt ggtgaaagaa tacaacaaac acttatctct
    14401 caacttcacc agttacatat tgatttaagc caatcatatc aaacgagcca acaatttatt
    14461 aatgaaaaga tagctcaagg acaactgact acacaacagc tcattagtga tgccatacaa
    14521 agacaaatgg ccgatgtcag agagcaaatg actcatagct tcaaacatca tgcgagctca
    14581 ctcacttctc acttacaatt actaactgag gaaattcgta atcatttaaa cacactaact
    14641 cagcaggtta atcacaaatt gaccgaaggt tttgagaaaa cttcgtctac ttttattgac
    14701 gtggtaaagc gattgaccat tattgatgag gcacaaaaga aaatcactga actctccagt
    14761 catgttgtca gccttcagga cgtattagtg gacaaaaaag ctcgcggtgc ttttggtgaa
    14821 gttcaattat caacactcat cagcaatatg cttcccagcg caagttatca aatgcaatat
    14881 accctcagta atcaaaaacg ggctgactgt gttttgttct tacccgagcc cactggaaat
    14941 attgtcatag acgccaaatt tcccctggaa acttatcaac gattaatcaa cagtgatgcc
    15001 ttatccacag atcgtaagtc attacaacaa caatttcgcc aagacattca aaaacatatt
    15061 aaggacatcg ctgaaaaata tatcgtgccc aatgaaacaa cagatggcgc catgatgttt
    15121 attcctgcag aatctatatt tgctgagatt catgcaaact atcctgattt aattgccatg
    15181 tctcaaaagc taaaagtctg gctggtatcg cctagcacat taatggctgt actgactaca
    15241 gcaagggcag tacttaaaga tgatgcaact cgcaaacaag tacatatcat tcaaaaacat
    15301 cttcaagccc tggccaatga ttttcaacgc tttgaaaaga gaatggataa attatccaaa
    15361 cacattgact tggcacatca ggacgttaat gaagtcaata catcagccaa aaagatcact
    15421 tccagattcc aaaaaattga atcggtcgat atcgaattgg atgaacacga agtaactcaa
    15481 ttagaattgg tcgagtctga atccatctaa aataaaatct ttatggtgca gaatacctaa
    15541 tttagcatta tactaagcgg ttttcagttt taatcattaa attccaatga atacaaaaac
    15601 gctactttac caacccacaa aaaataaaca aatatggggg caactctatg gtagcagtct
    15661 ggcacttgct ttagccgaat attgccaaca aacacccggg atcaaattac ttattacgca
    15721 agacaaccta tgcgccagtc agttgcagga tgaactacaa ttttttctaa acccgaacca
    15781 aaatacccag gagttattat tttttcccga ctgggaaacc ttgccttatg atcaattctc
    15841 tcctcatcaa gatattattt cagaaagact ctacacctta agcagagttc aacaaattac
    15901 caatggaatc attatttctt cagccagcac tttaatgcac agactttctc cgccagagtt
    15961 tttaaatcaa tacgctttaa tgcttaaaga ggggcaaaag cttgatctga actcgttcag
    16021 gaatcagttg caacaagcag gataccattg tgttaataaa gtgcttgaac atggtgaata
    16081 cgcactacgt ggatctatta ttgacgtgta tccgatgggt agtggtttac ctttccgtat
    16141 cgagctattt gatgatgaaa ttgaaagtct cagagaattt gatccggaaa cacaacgcac
    16201 aatagaaaaa atcaatcaaa tccatttgtt acctgctcgt gaatttccat taaatgaaca
    16261 aagccaatta cacttccgac gagctttcag agagtctttt cctggaaatc ccagccattg
    16321 cccaatatac gaagcaatta gtaacagtca atttccatct ggcatagaat attatctgcc
    16381 cttgtttttt gataaaaccg ttaccttttt tgattatctg ccagccaatg ccaaaatttg
    16441 tttaattgaa gaaacccaag agaatgctga gagattctgg cgagaattaa atgaacgcta
    16501 tgaacaacgg cgtcatgatg tcagccgccc tattttaacc ccagataact gctttattaa
    16561 tcccaccgaa ttattaacta aagctaacaa ttatgaacaa gtacgcctgt atcataagcc
    16621 ttccgaaaaa aaaggagcct taaatttcaa tattgccaaa gctccacaac tccccattga
    16681 caggaaaaat attgagccac tccgccaatt aagtgagtat tgttctgacc caacgcgacg
    16741 ctatcttatt gttgttgaaa gcgctgggcg tcgcgaagta ttactggatc tgcttaaatc
    16801 aagccatatc attccaagaa cacaaaactc gtggttggac ttcatttacg atgaatcccc
    16861 catcaatata actactggcc cattgattta tggctgtgaa ttaaccgaca aacaaattgt
    16921 gatcatcgtt gagtcgcaat tatttggtga acaaagcaca cctcaacgcc gaagcactca
    16981 aaaatcagtt gatcccgact taatcatacg agatatggcc gaacttcgat tgggcgctcc
    17041 tgttgtacat cttcaatttg gagtaggtcg ctatcaaggt ttgcaataca tcgactccaa
    17101 tggtaccccc agtgagttcc tcgtccttgc ttatgcaggg gatgacaaaa tctatgtccc
    17161 agtcacttct ctgcatatga taagccggta tactggtgtg gacagtgaac acgccccttt
    17221 gcataaactg ggttctgatc aatggcaaaa ggaaaaaaag aaagcggccg agaagattca
    17281 tgatgttgca attgagttat tggatctcta tgctaaaaga gaagcccagc cagggcacca
    17341 atacgatttc aatcaatccg aatatataaa attcgccagt ggcttcccct ttacagaaac
    17401 accagatcaa cttcaggcta ttgaacaaat tattagggac atgcaatcac caagaccgat
    17461 ggatcgccta atctgtggtg atgtaggatt tggcaaaaca gaagtcgcca tgcgtgctgc
    17521 attcgtagca gtacaaaatg gaaaacaagt ctgtatcctt gctccaacta ccttgcttgc
    17581 aggacaacat tttgagtcat ttcgcgatag atttgcagag tttcctgtta atattgaatt
    17641 attatcccgc tttcgcagta ataaagagtc cgaagcagtc cttgccgcat tgcaatcagg
    17701 taaagtagat attgtgattg gaacccataa attattccaa aataatattt ctttcaaaaa
    17761 cctgggatta ctgattattg atgaagaaca tcgatttgga gtgaaacaaa aggaacatat
    17821 taaatcgctt cgtactcatg ttgatatttt atccatgact gctactccaa ttcccagaac
    17881 cttgaacatg gcaatggcgg gaatcagaga tatttcactt attgcaacac cgcccgccaa
    17941 gcgtttggcg attaaaacat tttggcaaga aaaaaatgat ctcaccatac gagaagcgat
    18001 attgcgcgaa atattaaggg gagggcaagt attttacctg cacaataatg tacaaactat
    18061 agaaagagtt tgccaggatc tggaagcttt agtgcccgaa gcaaaaattc aaagcgcaca
    18121 tgggcaaatg cgagaaaggc aattagagcg catcatgtct gatttttatc atcaccgatt
    18181 taatgtcctg gtttgcacaa ccatcattga aacagggatc gatattccaa cggccaatac
    18241 cattattatc gatcgtgccg ataaatttgg attagcccag ctgcatcaat tacgtggtcg
    18301 tgttggacgc tctcatcatc aagcctacgc ttacttatta actcccaatg aaaaactgtt
    18361 aacgccagat gcagtgaaac gtctcgaagc cattgtgtct ttagaagatt tgggagcagg
    18421 gttcaccttg gcaacacacg atctggaaat tcgtggagca ggtgaactcc ttggtgaaga
    18481 acaaagtggt aatatgcatg ccataggatt taatttattt atggaaatgc ttgaccgtgc
    18541 cgttaacgat ttaaaggcag gtaaaactcc tgaattgtca gcaccaatgc atcaaggacc
    18601 tgaaattgac ttgagaatta gcgcgattat ccccgaagat tacataggtg atatacacaa
    18661 tagactcatc atgtataaaa gaattgctaa tgcaaaaact acccaacaac ttagagaact
    18721 acaaatagaa ctgatagacc ggtttggttt actcccacaa cctgtcaaac atctgttttt
    18781 gattactgaa ttaaagttaa aagcagcaca gctcggtatt caaaaaatca gtgccagtgc
    18841 tcaacaaggt aaattggatt ttagtgaaaa accatcgatt gaccctggaa cactgattag
    18901 cttaatacaa gtacatgcca aacgctatca aatggaagga ccgcaacgtc tgcgttttac
    18961 tctagacagc acgtcagcag aggaacgaat atttgaaatc agttctttat taaataaact
    19021 gtctcctcat tcccaaacga ttgactgaga ttgctgcagt gccttttgga ttaactcggc
    19081 ataatctttt tcaactaccc ttctctttac tttcagtgta ggtgttaatt tatcattttc
    19141 agttgtccag acatctttca gcacaaggat gtgactgact ttttcgtatt taaccaattt
    19201 cgaattgact tgctttaagg tttcttgcaa tgagtgagtg atctcctctt ttggcatacc
    19261 caaacgcact ccttcattca aggtaactaa caaaacgtta ttaggtaact ctctacctac
    19321 caggcacagt tgttctagca tagagttcac tgaaaacagt ttttctattg gtgaaggagc
    19381 aatgaactct cctgtctgat ttttaaagtt ctcagaaagt cttcctaata ttttaactct
    19441 gtcttcatta tcaacatcaa cgacatctcc tgttcgcaac caaccctctt ccgtaaaggc
    19501 atttttagtc gcttgttcat ttttataata acctgacatt agacaagggc acttcattag
    19561 taattcattc tcttcgccta tttttatctc aacctccagc ctcggagtac caacgtaacc
    19621 acgttttctc tcatttaaca tcgataaggt agcataagcc aaattctcag actgaccata
    19681 tccttcctga atataaattc ccaatttttc aaaaaaatca ataatggata ttggcagatg
    19741 agatgctccg ctaaaacaat tagtacactc gcttaatcct aaactgtgaa tgatttttct
    19801 ttttatcaaa ctggatataa gaggaatttt taataaaaaa ttcaatttgg ctggtggcag
    19861 tttttgttca attttctgct taaatactcc ccaaatgcgt ggaactgcag tgaaaaaggt
    19921 aggactcact tctcttaagt tctctgcaaa cttctccaga ctttcaacaa aagataccga
    19981 tgcattaata gcaacactgc ctaattggat ggcagagcgt tcatagacat gggccagggg
    20041 aagataagat attaatttat aatgttccaa ctctctgatt ctgagcaagt ccttggggaa
    20101 aactgtcaag taattggcta tggaccgatt ggtatacata gctcctttag gtgcccccga
    20161 agtcccggat gaataaataa tggtatacaa cgcatctggc tctggttcca ccaggttaat
    20221 catagggtca cttttcaaaa catcagacca ttcgtaatca acttgcaaat ctttatggta
    20281 atcaaaacca attgtaggat aacttttagg gataaattgt ctgacccgct gatgatcatc
    20341 taattttcca acaaaaacca gtttcacatc cccatgatct aaaacataat gcgcactttc
    20401 ttcattttga ttagcaaata acgggacatt gaccattcca gcaatatgaa ttccaaaatc
    20461 agcgataaac cattccgcgc aattcttgga gataatggaa atatgatcgc ctttttttaa
    20521 ccccagatgg tgtaaaaaac cagctacttt tcgggcctgc agcatcacgg ccgaccaggt
    20581 atattcatgc caaactccac tttttggttg tcttaaatac acatggtctg ccgaattttt
    20641 ttcatttttc aacagtaagt taaataatga attggaattg gattcttttt gcatggctct
    20701 tccttaagct tgtttaatta ctattctaga ccaactagtt gtaaaatatt atttttttct
    20761 cgcaaaacac aattttcacc ttcatgaatc gcctcttttg ctttatgaaa atcataaagc
    20821 atgatgtttc ctaattgagg aaccaaagtc aaatcagcag gatcgcctgc cgctcttgct
    20881 cgggttatcc ggtcttgcat tattttgatg cttctggtca agacatcata atatccaggc
    20941 ccatcactga ttcccattat tttttttatc attttatcaa attggcctga gagaactgaa
    21001 aaaaaacccg ggtgttctgt cgtgctcgga ggcaccctta gcaagtcatg atttaaattg
    21061 acagcgatta caacatctgc ccccatagct cgacacaatg taacaggaac gggattaact
    21121 aaaccaccat cgactaacca gcaatcctta taacgctgtg gggtaaataa ccctggtaag
    21181 gacatggatg agcgaatagc aagctcaaga gaacctttac ttagccatat ctcacgtcca
    21241 gtactcaaat cagtagcaac tgaactaaaa ggtagcgaga gctcctcgat atttttttca
    21301 agaccaaaat ccttgaaaaa ggtcatcaat ttactaccag agataacccc gccacctaaa
    21361 aaattcacat caatgagttt agccatttct cttttattaa gctttaacac ccattgttca
    21421 aacagatcca gggtgccaca ggcataaatg gcgccaacca atgcgcctat ggaacatcca
    21481 gcgacacaat caatccttac acccaatcgt ttgattgatt gaattacccc gatatgagcc
    21541 cagccgcgcg ctgatccgct acctaaagcc aaaccaattt tagggtattg atttgtcaag
    21601 aataactcct ggaaatagtt ggtacacact tgactggcat tttcttactt ggttccgcca
    21661 accgtcaagg catcaatttt caaagtgggc tgccctacgc caacgggaac agattgtcct
    21721 tcttttccac atacgccaat ccctctatcc aatgctaaat cattaccaat catacttatt
    21781 tttttcatca catcagggcc attgccaatc aaagtagctc ctttcaccgg ttttgtaatc
    21841 ttgccattct ctattaaata agcctcactc gcagaaaata caaactggcc agaagtaata
    21901 tcaacttgtc cgcctccaaa attcaccgca tacaaccctt tttttacagt gcgaataatt
    21961 tcctgagggt cgtactgtcc tgacagcata taagtattag tcattctcgg cattgggaca
    22021 tgggcatagg actcacgacg acaattacct gttgattgca ttcccattag tttggcattc
    22081 aacttgtcct gcatgtaatt cactaaaatt ccattatcaa ttaaggtcgt acattgtgat
    22141 ggcgtccctt cgtcgtctat agtcagagaa ccacgtcgat tttctaaagt accatcgtcc
    22201 acaactgtaa cacctgcagc agcaacctgc tgacccaatc tgcctgaaaa agcagataat
    22261 cccttgcgat taaaatcacc ctccaaacca tgtccaacgg cctcatgaag aagtacccct
    22321 ggccatcctg ggcccaaaac aactggcatg gttccagcag gagctggttc ggccttcaaa
    22381 ttggttaatg cttcccgtac cgcttcacgc gcataactca aagcgttttc tttctcggta
    22441 aaataggaat aagctactcg tccaccaccg cccgatctcg ctgattctct tctaccatga
    22501 ctgtcttcaa caataacact gacattaatg ctaaccaatg gcctcacatc agccatcatc
    22561 tgcccatcca tattggcaac cattactact tcatagcaac cagttaaagt ggcattaacc
    22621 tgtatcactc gaggatcaat actacgggcc tctttatcaa tggcttccaa taaagcgatt
    22681 ttctcctgtt tgctcattcc ttccaaagga tttaaactct catatcgatt tacagaagcc
    22741 tttaatacct tgacggccgg tactgttttt gaaccagaaa gggctatgga gcgcgccgca
    22801 tcggcagcac gaagcatgga aggcagtaat atatcatcac aataagcaaa gccggtttta
    22861 tcaccgctta cagccctaat ccctaccccc ttatccagag aaaaattacc gcttttaacc
    22921 tctgaatctt ccaaatacca ggattcataa ctacaacttt ggaaataaag atcagcatca
    22981 tcgacgttcc gattaaacat ggtcttgaat aatttttcta ttccaacttc atccagagaa
    23041 gctggtgtca ataaaatatt ttttgctatc gcaagagatt ttgtcattta tatacctttt
    23101 acattaaaac atgatgttca acacacggaa attgtcttct caactgtgac aatctctcta
    23161 aatcaatatc agccagaatt actccctgcc cttcttcttt ttgagccaac actttacccc
    23221 atggttccac aaccatacta tgcccgtaag tatgacgccc attttcatga gtcccaccct
    23281 gattaggagc taagacataa cataaatttt cgattgctct tgctctgagt aagacctccc
    23341 aatgcgcagc tccagttacc gcagtaaaag cagacggaac tgaaagtaat tgtgcgcctc
    23401 tttgggttaa atattggtac aattccggaa aacgcaagtc ataacagatg gttaacccaa
    23461 ttttccccac tggcgtatcc actagcgcta tatcttttcc tgcctctata gttaacgatt
    23521 cctggtgttt ttcctgctcg gaaactatga catcaaataa atgaatttta tcatagcgag
    23581 caacatttag gcctttatca tcataaacta tacaacttgc tcttaccttt gaacccatac
    23641 tttttaacgg gattgttcct gctattatcc atacccgcaa atctctcgct aactcactta
    23701 ttctttgttg tataggtcct tgaccataat attcagcaat gtgcaacttc tctcgttcat
    23761 tcattcccat aaaagcaaaa ttctcaggta gcaccaccaa actggcttcc tgttccctag
    23821 cttgaattaa atattgttca gtgagttgca aattatctgc tattttggca gatgatacca
    23881 tttgcacaag cgctactcga ttcatccttt ttcctaaatg acattcatat ttttcatctt
    23941 actatatcgt tccatataag cccaaaacaa aattgacaca aaaacctttt cgctttaata
    24001 ataaattgtc tattggtcta tatgtaaatt ttattacaaa attttcaagt aatgcgactt
    24061 gaaaaatagg aaatttgacc atagatatgc tacactgata ataaataact ggagttctga
    24121 acaatgaacc gaaatgatat caccatactt agtcaacaaa gagaatctgt gctcgcaacc
    24181 aataaggtcc tgcgaaatac gtatttactg ctaagtttaa ccttcatatt tagcgcatta
    24241 actgcctacg tagcctttat tagtggagct cgcccaatga atccactatt gatgatagtt
    24301 ggcgtttatg gtttgatgtt tctaactcaa gctttaagaa atagcgtttg gggtttggta
    24361 agcgtctttg cttttacagg gtttttagga tataccattg gcccattact caactattac
    24421 atcacaggtt tttcaaatgg cccacaaata gtcgctacgg ctttaggtgg tactggaatg
    24481 atctttttcg ctctatcagg atatgccctg acaacgaaaa aagattttag ctacctgggt
    24541 ggatttcttt ttgttggcgt tatggttgcc ttattggcga tgatagcggg aatatttata
    24601 caaataccag ctttgcaatt agtgatttct gctgcttttg tattaatttc atctggattg
    24661 attctattac aaaccagcgc cataattcat gaaggagaaa ccaattacat aatggccact
    24721 attggtttgt ttgtttccat ttataactta tttgttagcc tgctgaacct gttaagcgca
    24781 ttctcaggtc gcgactaatt taatatcgtc tcctctacct gagtcccggc tgtatgccgg
    24841 gattttttta tggatcaatt gtttaaaagg aaatgtaaga gaatatttca acatggaggc
    24901 tctggtctgg tacaaggcaa tttatctata ctgagactac ctgcgccttt taattgtcaa
    24961 ccgttcacgg ctatgtaatt aaggaatatt aagttctatg tcttttccgc gaaagcggga
    25021 atccatctat aaaattagca cgcaggttaa tttttaatta gattccctcc ttcgcgggaa
    25081 agacaactca aataaatagg cttataagca gaaaaattct catatcctgg acagttacta
    25141 ataattacat tataaaatcc aggagtatga tcaatcgatg tgaaattaat tttgtttcca
    25201 actagccttg gctttaaaaa gttctggcaa atcttccagt cttttcttca cttcctggct
    25261 cagagtaaaa agctcgcgct ccttttgata atagactccc aaaggtacgg ggtaatcagg
    25321 gaacgtcaat cgcgctaaac gcatggcgct aattaagttt ttctcatcat gcttataaag
    25381 cgttttttca tcaattgaaa tttgtacaaa ttgctcgtcg tccaactgca gcgctttttc
    25441 tttttgagcg ccaaatacga gtggctgacc gtcttccaat attattgtgt tttcagcccg
    25501 gttattttta actgcaaaat catcaaaagc accatggtta aaaatattgc aatcttgata
    25561 tatttcaaca aaagagcacc ccttatgttc ataagctttt ttaagcacag aagccaaatg
    25621 aacaggatct ttatcaaccg ctctggctac aaaactagct ccagaagcca aagcgatcat
    25681 gattggatta attggctcac ttgtcactcc tttgggggat gttttagtta cttgcccttt
    25741 ctgagatgtt ggagaaaatt gccccttggt taatccgtaa acctgattat taaacagaag
    25801 tatattgaca ttgacattac gccttaaaat atgcagcaag tgattgccgc ctatacttaa
    25861 cgcatcccca tctccggtga taacccagac acataaatct tctcgcattg cctttaaacc
    25921 agttgctact gctgtagccc tgccatggat agtatgaaac ccataagtgt tcatataata
    25981 aggaagccgt ccagcacacc ctatgcctga gacaaaaaca tgttgctcag gaggcaaacc
    26041 taattcagga agtactcttt gcaaagcagc caaaatggca taatcaccac atccaggaca
    26101 ccagcgaact tctgtttcat tcgcaaaatc ttcacgctta taattgctgt tcatagttgg
    26161 cttccgcttt aatggcactt accaaatgat taacactgaa aggttgacca ttgcattggc
    26221 taatcgattg cgcatccacc agataaactg ctctgatgag ttgacataat tggccagaat
    26281 ttaattcagc aaccaatacc ttgtcatatt ttttcaataa agaacccaaa tcatcaggta
    26341 atgggttaag atggcgcaga tgcagatagg caaccggtaa tccctgttca agacattgtt
    26401 gtacagcaga ctttaaacta ccaaaagtac taccccaacc tataacaagc gtgggagccg
    26461 tcatatcgcc ctcaagaacc aaagcaggat aatctcttgc aatacccttt attttctcgg
    26521 cgcgtgtaat caccattttt tgatgattct cagcatcata actaactcgt ccttcatcgc
    26581 cttgcttttc caaacctcca atttgatgaa taaacccaga agttccagga acattccagc
    26641 ttctacttaa aaattcatct cgatgatagg gtttggtaaa acgattaaac tccggttttg
    26701 gtatattgag agaatccaaa gcaggaattt cccagggctc tgccgcgttc gccaaataag
    26761 catcaagtaa tacaataact ggagtcatat acttgatggc tatcctgaag gcttcaataa
    26821 ttgtattaaa acaatcagca ggggattgag ccgcaatcac aggtaaaggt gcctcaccat
    26881 ggcgaccata caaagcttgc tttaaatcac tctggctggt ttttgtgggc aaacctgttg
    26941 aagcccccgc tctttgcaca tcaactagca ccaagggcag ctccgtcaca acagccaagc
    27001 ccaggctttc acattttaaa tcaagcccag gccctgaagt acaggtcaga gctaaacgac
    27061 caccataagc agcccctatg caagaacaaa tagcggcaat ttcatcctcg gcctgtatta
    27121 actgtacgcc gtaatcagat aagcgagcac attcatgcaa aatagcagaa gccggtgtaa
    27181 ttgggtaacc tgataccaac agaggaactt gtgtctgagt ggctaatgta gccagagcta
    27241 accctactgc ctcaactcca gtgatttgcc tgtactcacc tgaatgtctg ttgacttccc
    27301 ccagcatgta atcccgacgg gaaagctcca gagtcatagc ataattatat cctgccaaca
    27361 aagccagttc attggctttt gctatggcct gatttgtttt aaacttttta gcgataaaag
    27421 cctggcaagt ttctgtaggc aaatcaaaca accacaaaac caatcccaaa atataaaaat
    27481 tcttggtttt gacagcctgt ggttttgtaa ggttaatgga ttcaactgcc tgaatagttt
    27541 gtgtaattaa tgggaaagca acaatgtgat aatgttctcg acaattatcc aggaaactct
    27601 tatcgatacc tgctttttgc caatctcttt cttgaaaact gtcttcatta atgatcagca
    27661 aaccaccttg attcaaatgt tgaagtgagt tttttaatgc cgctggattt aaagccacta
    27721 aaacatccaa tgactctccg gcagtaaaaa tagctctttc cgccattgcc aactgaaatc
    27781 ccgagacacc cgcaactgtt ccagcaggag cccggatttc tgccgggaaa tcaggcatgg
    27841 tacgtacatc acgtcctgtc aatgccgcgc taatggttaa ttgttcacct accaattgta
    27901 ctccgtcacc agaatcgcca gtgatacgta caacaatgac atcagtcgtt gacataaagt
    27961 ctcgcttcct gcattaattg gcgcatgtgt cttactgcct cttttaatcc acagaataat
    28021 gctctggcaa taatggcatg tcctatgtta agttcattta actccctaat cgctgcaatc
    28081 ggtttaacat tatgatagtg caacccatga cctgcattca ctaccaagtt caaactggca
    28141 gcaaattcag ctgcctcttt aattcgttgc aattcataat gctgcttttc ttctgaggta
    28201 gcatcagcat aacaccccgt gtgcaattca ataactggcg ccccaacatc aaccgccgct
    28261 cttatttgct ctttatccgg gtcaataaat aaagaaacct cacttcccat taattgtaaa
    28321 cgacgtactg ccctttccac cacattgcgg tgagtcaaaa tatctaatcc accttctgta
    28381 gttagttctt cccgcttttc aggcactaag caagcatgct ccggcaatat ttcctcggca
    28441 aaatcaagca tggcttcagt aaccgccagc tcaagattca tacgtgtttg cagaacctgt
    28501 ttaatcaatc tgacatcacg cgcctgaata tgtcgtaagt cctctctcat atgcaaagta
    28561 attccatctg ccccagcttc ttcagcatcc attgctgcct gaacaggatc aggataacga
    28621 gttcctcgtg cttgtcttat ggttgctata tgatcaatat taacacctaa caataactct
    28681 ttattcattt tgcaccaaaa aatataaaat gcagagtata atgcagttct tgacaaactg
    28741 ctattcacgt taccttgttt caatcagttt aaacggc
//
LOCUS       DAXLDT010000027        28532 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXLDT010000027 DAXLDT010000000
VERSION     DAXLDT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 28532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28532
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.027"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1740
                     /locus_tag="ACF2SP_000933"
     CDS             <1..1740
                     /locus_tag="ACF2SP_000933"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFD2358735.1"
                     /translation="LIVVTTLVTLFYYLPDDLMLFSKSARKTSIFTSNVFFKNVTSDY
                     FSPDSNQLPLLHMWSLSVEWQCYFILPVGIYLLYRLVGPQHRIKIIYLLTITFFALSM
                     YYSWSNPAKTYYQFSSRIFEFLIGSCVASASGSKRFLANKYVLNFITVLAVLTLFYIA
                     MHHDINIGFPNWYTLILCLATAILIAAGQQEPQPIVTYALSLRPLVFIGLISYSLYIW
                     HWPVLALMRYLNIEESNLVLLVSFGLISIIAYLSWRFIEKPARKLHRIRFSYTVVCLL
                     IMPVFVTHLSDYLIKKNAGYPHRFNEAIQVYAELNKYKNPQRPLCLERKNTEVNPQCV
                     LGAKNSDSRTGFMIGDSYSNQHWGFMDTLAKEANVSILAHATVACLALPDIYQFDWNV
                     KNKIYQECHDQTNRYFNMIKTNHYDYVILGQNWNGYFGDRIINQLNDNRSQELSLKRI
                     EKALDKALELITTSGAKPVLIKSIALSKGNPYNCFFEHIKLHKKYNPERCDFNLDVSE
                     QIWFDNLFAKMQRKYKTLVIIDPRVAQCPSGMCKVDIKGIPVFRDSGHITDYASYHLA
                     KLYLQKNNNPLVS"
     gene            complement(1951..4230)
                     /gene="topA"
                     /locus_tag="ACF2SP_000934"
     CDS             complement(1951..4230)
                     /gene="topA"
                     /locus_tag="ACF2SP_000934"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFD2358736.1"
                     /translation="MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSV
                     NPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERN
                     LLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEE
                     KLQQFSVTQQEQAHEIKKQLIAQAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARK
                     LGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGD
                     NCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQ
                     MADAILDTVSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFN
                     VGEKIKVSDIETNQHFTEPPPRYSEATLVKALEEYDIGRPSTYASIIHTLQQREYVVV
                     EKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVLEEFWQP
                     FVQQIQNIDEQVQRKDVTTELLEEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDI
                     SNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGV
                     TCPKCNQAKILQRKSRKGKIFYSCGNYPKCDYALWNEPVDLPCPKCAWPILTLKESKK
                     FGRQILCPREGCDYSAKED"
     gene            complement(4310..4747)
                     /locus_tag="ACF2SP_000935"
     CDS             complement(4310..4747)
                     /locus_tag="ACF2SP_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFD2358737.1"
                     /translation="MKDNLFEMLLNLFEKSLTQLQKSHKSNDQDSQSQLLEEDLLSAE
                     EQSLFIKSAQQKSTRIFTYDEQMKLTKASYQFLMRMKLWNILDIDQFESIINQLQFSE
                     SRIVTLQETKWTIRNALASSLNEDQLTFLDLVLYQTEDELTIH"
     gene            complement(4737..5822)
                     /gene="dprA"
                     /locus_tag="ACF2SP_000936"
     CDS             complement(4737..5822)
                     /gene="dprA"
                     /locus_tag="ACF2SP_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFD2358738.1"
                     /translation="MNNLQPLLALNRMKRIGPRTVLKLQKRWPDLNLMFQLSSVDLEE
                     AGLPSWLAQTIKNFDPDFIKTDLNWLSSSENHHILTWDSPYYPALLKEIADPPFILYA
                     KGELSALTQPSLAIVGSRNASVTGNENARAFSREISRHGVSIVSGLALGIDAHAHLGC
                     LEGGGKTIAVLGTGIDCIYPRRHLKLAEQITENGLLLSEFPLKSPPIAGHFPLRNRII
                     SGLSLCILVIEAAIKSGSLITARMALEQNRDVLAIPGSIHNPLARGCHYLLQQGAKLV
                     TSVADVLDELKIEHHQFTSNKPIFSLASGKENLVKFIGFETTTIDQIIDRSGYGMEQV
                     TSGLAELELKGAVMAVPGGYIRCEYER"
     gene            complement(5819..6856)
                     /locus_tag="ACF2SP_000937"
     CDS             complement(5819..6856)
                     /locus_tag="ACF2SP_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD2358739.1"
                     /translation="MRYCLLIFCFIISSITHALSLRPDSPSRYVVQPGDTLWSISSRF
                     LNNPWEWKALWRANPQIQNPDRLYPGAVLALEYYQNIPYLRVLSNGTIKLSPNIRVTP
                     QEDAVPPIPLGDIKPFLDESLILDVNVLSLAPYVVALMSERMLGGQGDEIYVRGLHPS
                     KEMPQGGTIGYSIFRGGRNYFDPITHELLGYKAVLVGYGELVAGGEPATVLLTSINQG
                     IKINDKVLINNHPEFELYFEPETPARQVRGYIIEMPDNMPVGNTQEAVGGVIIINLGE
                     TSGIKPGDVLAIYGKERIVNDPQNHLRPITLPQERLGEAMVFRVFTKASYALIVRSTR
                     AIHLLDTVTNP"
     gene            6950..7462
                     /gene="def"
                     /locus_tag="ACF2SP_000938"
     CDS             6950..7462
                     /gene="def"
                     /locus_tag="ACF2SP_000938"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFD2358740.1"
                     /translation="MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARG
                     VGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVV
                     RAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
                     KFKRLQARKP"
     gene            7459..8403
                     /gene="fmt"
                     /locus_tag="ACF2SP_000939"
     CDS             7459..8403
                     /gene="fmt"
                     /locus_tag="ACF2SP_000939"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFD2358741.1"
                     /translation="MNDLTVVFAGTPEFGLPCLDALIQSRHHLKAVYTQPDRPAGRGR
                     KLQESPVKEWAINHQIPVYQPLNFKNQEAVDELSALKPDVMVVIAYGLILPKAVLEIP
                     RLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTS
                     SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQ
                     SAVEIDRKIRAFNPWPVAYTLAGELMLRIHQAKATDIMSTEMPGMVLNVDKNGMLVAT
                     NDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ"
     gene            8400..9683
                     /gene="rsmB"
                     /locus_tag="ACF2SP_000940"
     CDS             8400..9683
                     /gene="rsmB"
                     /locus_tag="ACF2SP_000940"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFD2358742.1"
                     /translation="MKVNDRFNALKILTALMESRKNLSYLMSPQEISPMTKEICYGFC
                     RHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKS
                     WAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAEAND
                     RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGS
                     VSVQDAAPQLAAYLLSLKPGQRVLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQ
                     RVRDNLNRLDLQATVVEGDVLEPRSWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRT
                     DEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQISRFVAEHPDCQVSD
                     GKWSWGRWTGHGQQILPGEHGMDGFFYSVLRKDEK"
     gene            9680..10429
                     /locus_tag="ACF2SP_000941"
     CDS             9680..10429
                     /locus_tag="ACF2SP_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFD2358743.1"
                     /translation="MIIDWPVIIVLFCLSIPGVLIVMHRLVYFLLADNTERLKKRFSR
                     FVVLQTLFMTFVMSFAGSILSSRTGLGDPLLEGLLQGKASLDSFQVILLPTFLYALLG
                     LVIFCILYYGLFGSILDEKSFKIMSELRSKIGLDGCVLYGGVVEEVIARWGLMNVVCF
                     FALLFTSQINSEIIIISIFLSAFIFAVGQLPAYIAAGCTPSRRFLYSFVLLSLSQSIL
                     FGFLFWQYGLTSAILAHMLFHLGWDRYEKLS"
     gene            10602..11105
                     /gene="ceg33"
                     /locus_tag="ACF2SP_000942"
     CDS             10602..11105
                     /gene="ceg33"
                     /locus_tag="ACF2SP_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg33"
                     /protein_id="HFD2358744.1"
                     /translation="MAAQLDPSSEFAALVKQLQREPDNPGLKQAVVKRLPEMQLLAKT
                     NSLALFRLAQVYSPSSSQHKQMILQSAAQGCTNAMLSACEILLKSGAANDLITAAHYM
                     RLIQSSKDSYIIGLGKKLLEKYPDFAEELKSKSKEIPYQSTLRFFGVQSESNKENEEK
                     IINRPTV"
     gene            11222..12013
                     /locus_tag="ACF2SP_000943"
     CDS             11222..12013
                     /locus_tag="ACF2SP_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFD2358745.1"
                     /translation="MQTRFTYLPVTSLQAGQYQPRQDFNVTELQELAQSISSQGLIEP
                     LVVRPIAKERYEIIAGERRWRAAKLAGLNEVPCLIGDYSDQQACALTLIENIQRQDLN
                     LIEEATGYKRLIEEFHYHQDEIAALVGKSRSHVANILRLLTLTDKVKQLVRDKVLSFG
                     HARVLVGLNPEQQEMFAHQIIGEEWSVRYLEQEIKSYKNKDTNSPKNPRKDRDIERLQ
                     TILAEQVGAPVQIVNDGEDGGWLKVKFFDNDTLAGLLERLGLRYD"
     gene            12105..13898
                     /gene="dacB"
                     /locus_tag="ACF2SP_000944"
     CDS             12105..13898
                     /gene="dacB"
                     /locus_tag="ACF2SP_000944"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFD2358746.1"
                     /translation="MKRTLTGAFMWAVWSLSSHAASMQFEVDKLINRLNPRVNLGIVV
                     TDLTSGETLYKRNANRLYIPASNMKLFSEAAALMALGPDYQFKNQLSTSANQLQQGVL
                     HGNLYLHLSGDPSFSREDLKALLSSLKDWNITTIQGNVIIDSSLMSIPAYPPGWLTSD
                     LSYSYGAPIAPLMVDSNRLTITVNPGAKAGAPAIVEVDDGGGTINLNNQATTKASEKG
                     CGVGFYLDPENNLTVRGCVGLGQWAVQQRIAIKNPFVYAQGMIVSELAKSNIKLNGQV
                     LLGRAPAGTLLIATRYSKPISQLMADTLKPSDNLYADSLYLHAAAKIKGSPVDWKQAQ
                     PVIKNFLQQQTGIDLKDSNFTDGSGLSRYNLVTPAQTMALLKFLYQRFPLSYEYIAAL
                     PISGRDGTLQKRFKTPNQQGFVRAKTGTMTGMNSLSGYLYTANGHTLAFAMYINRLPG
                     KPAGPGRPLLDALCTYFLQQSPTSSRLARVLSPHSRIKFQFNPTQIELQRVHQAKWRR
                     LETAVRQVLRGQDVNVVYRGNELIVTDNQSNANSVWKALQSIGKKYSFAVALSSKVMP
                     VTPSGKPLLLWVQAPLSENKAERTWIIREAV"
     gene            14709..15866
                     /gene="legS1"
                     /locus_tag="ACF2SP_000945"
     CDS             14709..15866
                     /gene="legS1"
                     /locus_tag="ACF2SP_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegS1"
                     /protein_id="HFD2358747.1"
                     /translation="MNFSIRVILLLTCFVSLAFSAPKFLTISDIHYGAENIAKDGQDT
                     GKEFLDVTFKRFEELSQNVDFILHLGDLPTHSLFSTSKKEEYEQVVFHGLHEANPGQK
                     PMFYITGNNDSLLGNYQPFSSDNKSPLNLALDWDGACVHCDGLIIDDTHMRKDGYYSS
                     YVIPNNKEIILIALNSVQWTKTPVFLPKYPNQQRDAFVQLFWLEQQLKNHRAKQLLLA
                     MHVPPGTAYNGNRFWHDIYLDRFLTLLDKYHRSYDQITILFSHSHMEEFRKIKLSDGS
                     TIYAFSTPGISRAHHNNPGMKIFDLDKQMRVRNFTTYFTSDLYEWGNEHYQAMNIPDA
                     IFPNCQNKTIPQCLDTLSPEQVCNNLEAGLFYGVKSTRVPYQGCIKTYQIN"
     gene            complement(15883..17433)
                     /gene="plaD"
                     /locus_tag="ACF2SP_000946"
     CDS             complement(15883..17433)
                     /gene="plaD"
                     /locus_tag="ACF2SP_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family phospholipase PlaD"
                     /protein_id="HFD2358748.1"
                     /translation="MAQKPTKITHLVVLGDSLSDRGTLNKRELLGFIPMSYLSGLRSK
                     SPKGRFTNGFLWGDYVGATTAEQFEIEHIRRKLRLHNNARDNADIGDELLINDKARRE
                     NENSFSLNEDNHILFKGERFARFYCEGGLTSHDYSFSFTFNLVLFFTRLILATLGGKR
                     TQLLADDKKYNISNLEKSETLVVEWSGANDLITANDKPSRAAAEKAVNDRIQNIEALI
                     HNGYRNFVLFNLPNLSLTPRFQRKNAEEQRNASDCTEYFNQQLQTKSLELIKKYKELG
                     IPVNLSVFDVNEQFKEVYSNPEQYGFDRDKLKSPYVESDLFKENQKNPIDQKEHISPA
                     KGYMFWDDVHPTATMHDWLAERFKEKYYDKVFRFEPPLQAKHRHKETADALAKTTDSM
                     VKTDKPIKKLPEDIVLMLNAIHSRAQLMCQSTDAQRREKGELLKHFIFEIKCQHGNLE
                     NIYGLISAFTLNPDHSKIIKTHQNPMIDFFRNKVTTRSEDDIAALQKAVATYLQPKES
                     NEHLVKLT"
     gene            complement(17654..18748)
                     /locus_tag="ACF2SP_000947"
     CDS             complement(17654..18748)
                     /locus_tag="ACF2SP_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFD2358749.1"
                     /translation="MRVSKLLLLTLALSGNLFAQDIKVVGTLNQTLKAPQNKTLNTKP
                     AIQQIKLLKMQLSNSAIKALARKTDITLKKPNTIAISTALPSKVELGMNNVPVLNQGS
                     FGTCVTFAATAAVDAALNKGDYISQLCQLQLGLYLEKNGHSPSGWDGSLGRIVLSQME
                     NFGIVSKEKQKNLGCGGLTEYPDNEQPTPDHAMSLEQFHEMSEDPETNPVIYTPILDV
                     YQAVLDRTDTNKTINDIKIALNQKDRVTFAVLLLDFDLGVMGAVGTNKTQFDTWVLTP
                     EIARDIYLRPFFGGHEMIITGYDDNAVAVDDNGREHKGLFTLRNSWGEQFGDKGNFYM
                     SYDYFKVLTIEAQRIRALADNESEEESMTA"
     gene            complement(18950..19681)
                     /locus_tag="ACF2SP_000948"
     CDS             complement(18950..19681)
                     /locus_tag="ACF2SP_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFD2358750.1"
                     /translation="MKKIIALIFLSLISMASFSLPKGFVYLHDVAPDIIEDIRYATHN
                     NFIGNPIPGYKRGVCIVTRQTAQQLKKAEEYIKPKGYTLKVYDCYRPQRAVDYFYKWS
                     RNAKDNRMKPEFYPREIKQDLFDRGYIALTSGHTRGSTLDLTLVKLTASQEKKSNHPL
                     TRCYDKKPNYLDDDSIDTGTRFDCLDVSANIDYSKLSKNQKANRKLLQKLMSRFGFRP
                     YYYEWWHFTLKNEPYPDTYFNFPVE"
     gene            complement(19707..22445)
                     /gene="sidF"
                     /locus_tag="ACF2SP_000949"
     CDS             complement(19707..22445)
                     /gene="sidF"
                     /locus_tag="ACF2SP_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PI phosphatase SidF"
                     /protein_id="HFD2358751.1"
                     /translation="MPRITENIETYVTHLLSDLEPVPSQSQHLAYGYPGERQVFKDPM
                     LDGKQVVVVNSQYDKHGKPVTGQPDVIQEANNYIDNLVAAAKSLIDKDNKDKKDSRKN
                     TIDEMAKTFKKSISETIPSDKAKADLFSSKKSSANKDFLEQLAKVEGSLQQFTQAVAK
                     ASGHKLKALDKEGHNIRSHNRDTLIHRFKAPNSKEGEEQFIMYIPCGRYTKRQQRLMG
                     KDESEVNRDHDTTSHKRSDLVLGNSSMARMIAGTRHSDGTVTIHHDSFSGPGARMPYS
                     DFKGADDYKKLAIKAVTLINQEEVIQTLAQRQIDRMTNEDLWNKIPEEYRPDELPPDA
                     EKARAQLIKLYVEHNPLSVTECYTQAVTAGQRVAAENQKEQFEYVRQMMDAFDGSKAK
                     ITIQTGSNTEVETEVGYQARMSSWGVNWFRQVGALNPLSDNSVTKNQNARFVNQMTDD
                     VISNLDKAVQSLGDYDKAGALHTLLKGPDVSNLNQQIARKENDLKEVKEAYREALFSY
                     FEEYQKGEGKWDQAKLDQLKNQVDGYEKSIKKQESAIYELHNQIDALRKAYYTEHKGQ
                     INKALQELKEQISPVIQNKETDPETKSKLQHFYNSCAYLMQAQELYYENTWHHGKNNF
                     KLQTLMASLSCELDYANTKGCKSNNDRGQRLAQKIVGNALWTAMSEDGLFTGEFLDHR
                     RTHGKEVSKVEQLDRELTTIQALHHTANTGVSGGKFEIQDKANFADNGLFGKIANFAK
                     VKEMGPENAFTKNMGTKIKAGLGLGLLAAGGLVALTLLFPPAGLAVAALGIGVAAGAA
                     IVAGVTFAATYLFGTAIDAINNKIEEGKIKSIVREGMKAASENSPQHKTEQKDNLTVN
                     AKLHQQLGVTRESVLERQSTPKQEFEKPKDMTSKVDLTPAMEDDERSESTPTPVNF"
     gene            complement(22609..22788)
                     /locus_tag="ACF2SP_000950"
     CDS             complement(22609..22788)
                     /locus_tag="ACF2SP_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358752.1"
                     /translation="MKWIMLIVVIITTAGCCCTTAEVVEYQQVTVAAPVVEPVILDVD
                     DPEPLDVTTTTIEFY"
     gene            complement(22911..23177)
                     /locus_tag="ACF2SP_000951"
     CDS             complement(22911..23177)
                     /locus_tag="ACF2SP_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFD2358753.1"
                     /translation="MKRKKPVAVALRYDGNSPPKVTAKGEGLFAQQIINTAKEHGIPL
                     EQNPELTALLSQVRLNEEIPKALYIAVAQILAFLYYINGKHSKE"
     gene            complement(23174..24340)
                     /locus_tag="ACF2SP_000952"
     CDS             complement(23174..24340)
                     /locus_tag="ACF2SP_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFD2358754.1"
                     /translation="MSVEMSKTSSVRFPPGQEIKLAKPATELYVGQILKTVVVTTLSN
                     DQVLININGQNLNAKASHHFTPGELFEVKVIANHPETILEVQQKTLLPSLLQNALLQY
                     LPKQAPATNLLQTLTQLMAENNLSAPINQVMKAILGNVPVLTQLPNQLMQAISQSGVF
                     LESKLLKGKIPREIQSDFKGLCFKLLHSLGTDSKLPINLPNELESGFLQQAPLPLPGA
                     IPQPLTKDTILNLIGLSTEAVHHILREQVTQALARITTNQINYLSQENQEAYLIMLDF
                     PLKTPDGIDVIPIMIKQHTAEPMQLSKWSISFALNLSHLGSIQATVSIYAKNIDIKVN
                     TQQSSTLQLLNDYHKDLEETLYESGLHLREWKLQVGLEENHIDATNLRLLDIKI"
     gene            24578..26815
                     /locus_tag="ACF2SP_000953"
     CDS             24578..26815
                     /locus_tag="ACF2SP_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HFD2358755.1"
                     /translation="MLDRASVVDEQFLKRVLQGDFPQLRSSMSPDTAELDKKTAIEFF
                     DSQIKSRLLDIIARQLKEKGLSFYTIGSSGHEGNAVLGQVFRASDMAFLHYRSGAFFL
                     QRAKQLPGIDGVKDILLSLVAAANDPISGGRHKVFGSVPLNIPPQTSTIASHLPKALG
                     AALSITRARELGLPSKLAPDSVILCSFGDASTNHATSQATLNACSWITQQNYPLPIVF
                     ICEDNGIGISVPTPSSWIKSSVSSRPGIHYIECDGLNIADVFAKAQEADYLARVKKQP
                     VFLHMRCVRLLGHAGSDIESQYNTQEEIEQREANDPLLHTAGILYREGWMSLQAMVEL
                     YQDNRALIEAKALEAIREPRMSSAEEIMASIVPRVEKKPKYPLPDDNRREQVFAGAYN
                     QLTLKRNLCQQINFALTDLMMQYPNMLIFGEDVGKKGGVYRVTADLQARFGQRRVFDT
                     LLDETTIIGTAIGLAHNGFIPVPEIQFLAYLHNAEDQLRGEASTLSFFSSGQYQNPMV
                     LRIASLAYQKGFGGHFHNDNSIAVLRDLPGVIVACPSNGPDAAKMLRTCMRLAYEEGR
                     VVVFLEPIALYMTKDLYSPGDNGWLFEYPSPDEMISQGEVGVYGEGDTVILTYANGYY
                     LSRQAEKVLREVHNISVKIIDLRWLSPLPKDAILKEIAKAKRILIVDEGRQSGSISEG
                     LMTLLMEEASPRLKIKRITGKDCFIPLGTAWQYLLPSQESIIDAVIALQSDKREKESG
                     RLVVS"
     gene            26841..27950
                     /locus_tag="ACF2SP_000954"
     CDS             26841..27950
                     /locus_tag="ACF2SP_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFD2358756.1"
                     /translation="MIRDSVSRFVSNDVIPLMAESYEHAHFPKELIKKSADLGLLGLT
                     LPAEYGGAEASYVAYGLVCQELERGDSGLRSFVSVQSSLCMYPIFKFGSEEQKKRYLP
                     EMAAGNIIGCFGLTEPDSGSDPASMRTHAKKIEGGWILNGAKMWITNAPIADIAIVWA
                     KTEAGIRGFIVDTKSEGMSCPEIKLKMSLRASITGELVFDNVFVPDENLLPKSEKGLG
                     AALSCLSQARYGIAWGAMGAAMACFDIARDYLLERKQFAKPLASFQLIQKDLADMYTE
                     IIKAQCLNLQIGRLKDQHRETPTMISLAKGNACREALKIARKCRNLMGGNGISLEYHV
                     IRHMLNLESVFTYEGTDNVHTLVLGRHITGINAFG"
     gene            28146..>28532
                     /locus_tag="ACF2SP_000955"
     CDS             28146..>28532
                     /locus_tag="ACF2SP_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358757.1"
                     /translation="MDIRCKTVLYKQLQEFNMINMKLKATLIACLSVLTLSSYANSSE
                     NKEAILQQCHDLASTVASLVSSQAKKTCAEKLVIASLHIDTAADWIVEDVHSAAKQEL
                     DNAIYSLQYAELNSCNRYIQISHSKLE"
BASE COUNT         7921 a         5376 c         6087 g         9148 t
ORIGIN      
        1 ttgattgtag ttacaacctt ggttaccctg ttttattatc tgcccgatga cttgatgctt
       61 tttagtaaaa gtgcgcgtaa aacatctatt tttacatcga atgtgttttt taaaaatgtc
      121 actagcgatt atttttctcc ggatagcaat caattgcctc ttttgcatat gtggtcttta
      181 tccgtagaat ggcaatgtta ttttatcttg ccagttggaa tctatttgct ttatcgattg
      241 gtcggtccac agcatcgaat caagataatc tatttattaa ccatcacttt tttcgcatta
      301 tccatgtatt attcttggag taatcctgca aaaacgtatt atcaattttc cagtcgtatc
      361 tttgagtttt taattggttc ctgtgttgcc tcagcttcag ggagtaaacg ctttttagcc
      421 aataagtatg tattaaattt tataactgtc ttggctgttc tgacgctgtt ttatattgca
      481 atgcatcatg acattaatat aggttttccc aattggtata ctttaatttt atgccttgca
      541 acggccatac tgattgccgc cggacaacaa gagccgcaac ctatagtaac ttatgctctt
      601 tcattaagac ctctggtgtt cataggatta atttcctatt ccttgtacat ttggcattgg
      661 cctgtattgg ctttaatgcg ctatttaaac attgaagagt ccaatttggt tctgttggtt
      721 tcttttgggc tgatatcgat tatcgcttat ctttcctggc gatttattga aaaaccagca
      781 agaaaattgc atcgaatccg gttcagctat acagtggttt gtttattgat tatgccggtt
      841 tttgtcactc acttgagtga ttacctgatt aaaaagaatg cgggctatcc tcatcgtttc
      901 aatgaagcaa tccaggttta tgcggaatta aacaagtaca aaaatccaca gaggcctttg
      961 tgtctggaac gcaaaaatac cgaagttaat cctcagtgcg ttctgggagc aaaaaactcg
     1021 gatagtcgaa ccggctttat gataggcgat tcttattcta atcagcattg gggatttatg
     1081 gatacgttgg caaaggaggc caatgtatcc attttggctc atgcaacggt tgcttgtctt
     1141 gctttgcctg acatatatca atttgattgg aatgttaaaa ataagattta ccaggaatgc
     1201 catgatcaaa cgaatcgcta ttttaatatg attaaaacca accactatga ttatgttatt
     1261 ttaggccaaa actggaatgg ttattttggt gatagaatta ttaatcaatt gaatgacaat
     1321 cgatctcaag agctttcttt aaagcgcatt gaaaaggctt tggataaagc tctggagctt
     1381 attaccactt caggagcgaa acctgtatta atcaaatcga tagcactgtc taagggaaat
     1441 ccttacaatt gtttttttga gcatatcaag ctgcataaaa aatacaatcc cgagcggtgt
     1501 gattttaacc tggatgtcag tgaacagatt tggttcgata atttgtttgc aaaaatgcaa
     1561 agaaaataca agacactggt cattattgac ccacgagtag ctcagtgtcc gtcggggatg
     1621 tgcaaggtag atatcaaggg tattccagta tttcgcgatt caggacatat caccgattat
     1681 gcttcctatc atttggccaa attatactta cagaagaata ataatccctt agtgagttga
     1741 gataatttac tgggttaatg gattgcgaat aaaacaaaca gattagtaaa cgttcacggg
     1801 ttatgccatc tcttgaaaaa caaatgagat aaatgctgtc attgcgaacg aagtgaagca
     1861 atccagaacg atattttgtt agaattctgt actggattgc ttcacttcgt tcgcaatgac
     1921 ggaatttaca tgccccatga acgtttacct ttaatcttct ttagcagagt aatcacaacc
     1981 ttcacgagga caaagaattt gacgaccaaa ttttttactt tcttttaagg ttaagattgg
     2041 ccatgcacat ttaggacaag gcaaatcaac aggttcattc cacagagcgt aatcgcattt
     2101 tgggtaattt ccacaggaat aaaaaatttt cccttttctt gatttccgct gcagaatttt
     2161 cgcctggttg catttagggc atgtgacgcc agtatcggag ggtttttcta gaggttccat
     2221 atgcttgcat tcaggataat tgctgcatcc aataaatttt ccatagcgac cggttttaat
     2281 atgcaaggct ccatggcaca gggggcagct tctaccttcc acgacttcag gttctgattt
     2341 ttctccttct ggattgctta tatcctgagt gtaatcgcaa tccggatatc ctgtgcagcc
     2401 aataaagcgt cctctcttgc cgagcctaat ggacaatggc ttttgacatt taggacattt
     2461 ttcttctaat aattccgtag taacatcctt tctttgcact tgttcatcta tattctgaat
     2521 ttgctggacg aaaggttgcc agaactcttc cagaacaggg atccaatcct tttctccacg
     2581 cgcgatggca tccagtgtgt cttcaaggcc agcggtaaac tgatagtcga catagcgagt
     2641 aaaatagtta gttagaaatc gattgacaat tctacccacg tcagtgggca aaaaacgttt
     2701 tttttcaact accacgtatt cgcgctgttg caaagtatga atgatagagg cataagtaga
     2761 tggtctgcct atatcgtatt cttcaagagc tttaaccaaa gttgcttcag aatatctcgg
     2821 aggtggctcg gtaaaatgct gattggtttc aatatccgat acttttattt tttcacctac
     2881 attgaaagca ggtaaaattt tatcttcatt gtcttcgtct ttagaatcat ctcgtccttc
     2941 ttcatagaca gaaaggaagc caggaaaggc tattgttgaa ccattggctc tgaaggtatt
     3001 ccctttacca cagctaaaat ccacgctcac cgtatccagg atggcatcag ccatctggga
     3061 ggccactgtg cgtttccaga ttaagctgta gagtttcaat tgatcgcttg tcagggaacc
     3121 ttgcaccatt tctggagttc ttttgatgga agttggccta atggcttcat gtgcttcctg
     3181 tgcgtttttg gatttagttt tgtaaattct gggagaattt gggcaattat ctcccttata
     3241 acgttgtgtg atatagtcgc gtatttcgtc aatcgcttcc tttgccaggt tgactgaatc
     3301 agtacgcatg taagtgatta aacccaccgt tcctgtgcct atatcaatac cttcatataa
     3361 ttgctgagcg accatcatgg tttttcgagc ggtgaaacct aattttctgg cggcttcctg
     3421 ttgcaaggta gaggtaataa aggggggaga tggcttacgt ttacgctgtt ttttatcaat
     3481 ttgagcaacg gttaaaaacc cttgtgcttg tgctatcagt tgctttttta tttcatgggc
     3541 ttgttcttgt tgagtgactg aaaactgttg aagtttctct tcgttataat gggtcagtcg
     3601 tgcttcaaat tcggtgctgg catgagcgca ttttgcgatt attttccagt attcctgagc
     3661 aatgaaacgt tcaatttcct cttctcgctc aacaattaac ctaagggcag ggctttgtac
     3721 tcgtccggcc gataatcctc gccgaatctt tttccatagc aaaggggaca ggttaaagcc
     3781 aaccagataa tcaagagcac gtcgggcttg ttgagcattc accaaatcca tggatatgga
     3841 gcgaggatga ttaattgcat cctggattgc tgcttttgta atttcattaa aaaagattcg
     3901 atgaactgat ttgtccttta aaaggtttcg ttctttcatt aattcaaaaa tatgccacga
     3961 gatagcttct ccctctctat cagggtcagt tgccagcagt agagagtctg attttttaag
     4021 agtttttgct atcgtatcaa tgtgtctcgc atttttttca ataggaacat aagtcatgga
     4081 aaaatgtttt tcagggttta ctgacccttt acgagcaggc agatcacgca catgaccata
     4141 agacgctaac acgtcatagt catttcctaa atatttctgt attgttttag ctttcgcggg
     4201 cgactcgacg atcaccaagt gtttactcat atcaaataaa ccttatcctt tttgggttgt
     4261 gtggctcact aacagttcat gaatgggttg atatcagtac actcattaat tagtgaattg
     4321 ttaattcatc ttctgtttgg tatagcacaa gatcaagaaa agttaattga tcttcattca
     4381 aggaggatgc cagagcattt cttatagtcc attttgtttc ttgcaaggtt acaatacgag
     4441 attcagagaa ttgtagttga tttattattg attcaaattg atcgatatca aggatattcc
     4501 ataatttcat acgcattaga aattgatagc tggcttttgt cagtttcatt tgctcatcgt
     4561 aagtaaaaat acgtgttgat ttttgttgag cagatttgat aaacaatgat tgctcttcag
     4621 cacttaaaag atcttcttca agcaattgac tttgtgagtc ctgatcattg gatttatggc
     4681 ttttttggag ttgagtaaga cttttttcaa acaaatttaa taacatttca aataaattat
     4741 ctttcatatt cacaccttat atagccaccg ggcactgcca tcacagcccc tttcaattct
     4801 agttcagcca gtccactcgt gacttgttcc atgccatatc cactacgatc aatgatttga
     4861 tcgatagttg tagtttcaaa accaatgaac tttactaggt tttccttacc gctggcaagg
     4921 gaaaaaattg gtttattaga agtgaactga tggtgctcaa tttttagctc atcaagaaca
     4981 tcagccactg aagttactaa tttcgcaccc tgctgcaata agtagtggca gcccctggct
     5041 aacgggttat gaatcgatcc gggaatggcc aatacatctc tgttttgttc cagagccatt
     5101 cttgctgtaa ttaaagaacc gcttttaatt gctgcctcga taaccaaaat gcataatgat
     5161 agaccgctta ttatacgatt tctgagtgga aaatgtccag caattggtgg actttttaaa
     5221 ggaaattcgc ttaataataa accattctct gtaatttgct cagctaattt taaatgccgg
     5281 cgcggataga tacaatcaat tccggtacct aagacggcta ttgttttgcc gcctccttcc
     5341 agacaaccta aatgagcatg agcatcaatt cccaaggcta aaccactcac tatgcttacc
     5401 ccatgcctgg aaatttccct ggagaacgct cgggcattct cgtttcctgt tacagaagca
     5461 ttgcgacttc caacaatagc caggctgggt tgtgtcaagg cggacagctc acctttagcg
     5521 tataaaatga agggaggatc ggctatttct ttgagcaatg cgggataata gggggagtcc
     5581 caagttaaaa tatgatgatt ttctgatgaa cttagccagt tcaaatcagt ttttataaag
     5641 tccggatcaa aattttttat ggtttgtgcc aaccaggatg gcaagcccgc ttcttccaga
     5701 tcaacactgg acagttggaa cataaggttt aaatcaggcc atcttttttg cagttttaat
     5761 acggttcggg ggccaattct tttcatcctg tttagggcga ggaggggttg cagattattc
     5821 atgggttcgt tactgtatct aataaatgaa tagcgcgcgt ggatcttact atcagggcat
     5881 aacttgcttt tgtaaacaca cgaaacacca tagcttcgcc aagacgttct tgcggtaagg
     5941 taataggtct gagatgattt tggggatcat tgacaatacg ttccttgcca tagatagcca
     6001 aaacatctcc cggttttatc ccggaggttt ctccaagatt aatgatgatg actcctccaa
     6061 cagcctcttg tgtatttcca acaggcatat tgtcaggcat ttcaataatg tatcctctga
     6121 cttgcctggc aggagtttcg ggttcgaaat acaattcaaa ttcagggtgg ttattaatca
     6181 ataccttatc attgattttg atgccctgat taatactggt caataaaacc gtagcgggct
     6241 cgccgccagc aaccaattct ccataaccga ccaagaccgc tttatagcct aacaattcat
     6301 gggttattgg atcaaaatag tttctccctc ccctgaagat ggagtatcct atagtgcctc
     6361 cttgaggcat ttctttggag ggatgcaagc ctctgacata aatttcatct ccctggcctc
     6421 caagcatccg ctcactcatg agtgctacca catacggtgc taggcttagg acattaacat
     6481 ccaggattaa cgattcatcc aaaaaaggtt tgatatcgcc taatggaatt ggtggaacgg
     6541 cgtcttcctg gggtgtcact cttatgttgg gggatagttt gattgttcca ttagacagaa
     6601 ccctgagata gggaatgttt tgataatact ctaaagcgag aacagcacca ggatataacc
     6661 tgtcgggatt ttgaatctgg gggtttgcgc gccataacgc tttccattcc cagggattat
     6721 tcaaaaaacg gctggagata ctccataagg tgtctccagg ttgcacaaca taacgagagg
     6781 gggaatccgg tcttaggctt aaggcatgag taatggatga tatgatgaaa cagaaaatga
     6841 ggagacaata tctcatgtat caatcccttt agttgagcct tagcatcttg cagcattact
     6901 gtatatcggg gataatatca cgattaacgt gtggagaact agataaacaa tggcaattcg
     6961 caagattctt tatttacctg atgagcggtt aagaaaaata gctaagccgg tcgagacttt
     7021 tgatgagagt ttacagacat taatcaacga tatgtttgat acaatgtatg atgctcgggg
     7081 agttgggctt gccgcaccac aaataggagt cagcttgcgt ttatctgtca ttgatatagt
     7141 gggagataaa aaggagcaga tcgttattgt taatcctgaa atagtttcct ctcatggcga
     7201 aaaagaattt gaagaaggct gtttgtctgt cccaggggct tatgataccg ttgtcagagc
     7261 tgaaaaagtg accgtgaagg cactggatag gttcggcaaa ccgtttgaaa ttacgggtga
     7321 agggctgctt gccgagtgtt tacaacatga aattgaccat atgaatggca aattgtttgt
     7381 cgatatgttg tccccattga agagaatgat ggctcgccgg aaattagata agttcaagcg
     7441 tttacaagct cgtaaaccat gaatgattta actgtcgttt ttgctggaac tccagaattt
     7501 ggcttaccct gtctggatgc tttaattcaa tcaaggcatc atctcaaggc tgtttatact
     7561 cagcctgaca ggccggctgg ccgaggccgt aaattacagg agtctcctgt aaaagaatgg
     7621 gcaataaatc accaaatacc agtttatcaa cctcttaatt ttaaaaatca agaagctgtc
     7681 gatgagcttt ctgccttaaa gccagatgta atggtcgtga tagcctatgg attaatatta
     7741 ccaaaagcag tgttggaaat tcctcgtttg ggttgtatta atgtacatgc ttctctgtta
     7801 ccgcgctggc gtggcgcatc tccaatacaa catgccattt tacatggtga tgctgaatcc
     7861 ggagtgacca ttatgcaaat ggatgttgga ttggatacag gtcctatgtt atgcaaggcc
     7921 acctgtcctg tgacttcctc ggataccgct ggtagtttgc atgataaatt agcaaaaatg
     7981 tcagtaaaac cattattgga tgttttggag gctttagcat ccaattcagc acaatttgaa
     8041 ttacaaaaca atgagttagc gacttatgct ggtaaaatta ataaagaaga ggcacgaatt
     8101 aactggcatc agtctgcagt ggaaatagat agaaaaattc gcgcatttaa tccatggcct
     8161 gttgcctata cgctggctgg ggaattaatg ctcagaattc accaggcaaa agcaactgat
     8221 atcatgagta ccgagatgcc gggcatggta ttaaatgtcg ataaaaatgg aatgttggtc
     8281 gctaccaatg ataatgcatt gttagtggaa aagattcaat ttcccggagc aaaggtcatt
     8341 tcagttaggg attggttaaa ttcgggtaaa acacaattgc atactggttt aatgttgcaa
     8401 tgaaagtaaa tgacagattc aacgccttaa aaattttgac ggctttgatg gaaagcagaa
     8461 aaaacctgtc ctatttgatg tctccgcaag agatttctcc catgacaaaa gaaatttgct
     8521 atgggttttg tcgtcattat tttcgtttac aatccatagc tgatacttta atcaataagc
     8581 gacctaaaga taatgaggta tggatagtct tgttgatagg tatttatcaa ttgcagtata
     8641 tgaataagcc ggattatgcc gtggtgaagg agacagtgtc tttactgggg caggttaaaa
     8701 aaagttgggc aaaaggactg gtaaatgccg tgctaaggaa tttttgccgt cgacaagacc
     8761 atattctgga aaagttggca gcagaaccgg tttttatcta tggacagccg aattggttac
     8821 ttcaacgcct gcaaaaagac tggccaaacg attggcaatt catcgctgaa gcgaatgata
     8881 gacacccacc catgactttg agagtcaatg tgttgaaaca ctcagttgat gagtatcttg
     8941 atttactaaa aaaatccggg atagaggctt ttaaacaccc tgtcgcacct gatggaatta
     9001 ctttggcaat cccttgtgat gtgatgacgc ttcctggttt tgcgcaaggt tctgtgtctg
     9061 ttcaggatgc tgcgcctcaa ttagcagcgt atttattgtc tttaaaacct gggcagcgag
     9121 tactggatgc ttgctgcgct cctggcggaa aaacatgtca tattttggag aaagagtctg
     9181 gtttggcttc ctgtgttgca ttggatttag acccacagcg cttacagcgg gtcagagata
     9241 acttaaatcg attggatttg caagcaacgg tggtggaagg tgatgtattg gaacccagaa
     9301 gctggtggga tggtcatcca tttgatcgta ttttactgga tgcgccctgt tccgctacag
     9361 gagtgattag acgccactct gatatcaaat tattacgaac ggatgaagag atttcagcaa
     9421 tttctcaggt acaatccagc atgttgaatt cattatggcc actgcttgca ccaggtggtt
     9481 taatggttta tgccacatgc tctattatga gtgatgaaaa tgagttgcaa atctcccgat
     9541 ttgttgcaga gcatccggat tgtcaagtga gtgatggcaa atggtcctgg ggaaggtgga
     9601 ctgggcatgg tcagcagata ttgcctgggg agcacggcat ggatggcttt ttttacagtg
     9661 tattgcgaaa ggatgaaaaa tgataattga ctggcctgta attattgtct tgttttgttt
     9721 atccattcct ggtgttttaa tcgtgatgca tcgcctggta tattttctgc tagccgataa
     9781 taccgagaga ttaaaaaaaa gatttagtcg ttttgttgtg ctgcaaacct tatttatgac
     9841 atttgtcatg agttttgctg gctccatcct gtcttcacgt accgggctag gcgatcctct
     9901 cctggaaggt cttttgcaag gcaaagccag cctggattcc tttcaagtga ttttattacc
     9961 tactttttta tatgcccttt tagggttggt tattttttgt atactgtatt atgggttgtt
    10021 tggttccata cttgatgaaa aaagtttcaa gattatgtcg gaattacggt ccaaaatagg
    10081 acttgatggt tgtgtccttt atggaggggt cgtagaagag gtaattgcca gatggggatt
    10141 aatgaatgta gtgtgctttt ttgctctatt gtttacaagt caaattaatt cggaaattat
    10201 aattatttca atatttttga gtgcatttat ttttgctgta ggccaacttc cagcttatat
    10261 tgcagcaggt tgcaccccat ccagacgatt tctttactct tttgtattat tgagtctttc
    10321 gcagtccata ctctttggtt ttttattttg gcaatatgga ctaacctcag caattttagc
    10381 gcacatgcta tttcatttgg gttgggacag gtatgagaaa ttatcttaat tttcattcca
    10441 cgttttgatc aagatgggag ttttttaaca tttatgctct taaaatactg ttaaaaaaac
    10501 agttatgtgt aaaaaaaatg taaaagtgcc atctcttaat tttgtcttaa ttttgagcat
    10561 ttattctgtc tttaatgaag atgataatca aaggttgagg tatggccgcg caattagatc
    10621 caagtagcga atttgctgct ttggttaaac aattacaacg agaacccgat aatccgggat
    10681 taaagcaagc cgtggtgaag cgtctgcctg aaatgcagtt attagctaaa acgaattctt
    10741 tggccttatt tcgtctggca caagtttatt ctccctcatc atcacagcat aagcagatga
    10801 ttttgcaatc agctgcccaa ggttgcacga acgctatgtt atctgcttgt gagatattat
    10861 tgaaatccgg agctgcaaat gacttgatta ccgctgcaca ttatatgcgc ctcattcaaa
    10921 gctccaagga ttcgtatatt attgggcttg gcaagaaact gttagaaaaa tatcccgact
    10981 tcgctgaaga gttaaaatcc aaatctaaag aaatacctta tcagtccact cttcgatttt
    11041 ttggagttca atctgaaagt aacaaggaaa atgaggaaaa aataataaac agacccactg
    11101 tttagaataa gttttttctc gtttcagggt ttttctttga ggtcagataa accggataca
    11161 attccatttc aggcactatt gtgaaacaat ttcatatcct taagtttctt gattttttcc
    11221 tatgcaaacc cgatttactt atttacctgt tacaagcctg caggcaggtc agtatcaacc
    11281 caggcaagat tttaatgtaa cggagttaca ggaattggct cagtcaattt cttcccaggg
    11341 tttaattgaa cccttggtgg taagacctat agcaaaagag cgatatgaaa ttattgcggg
    11401 ggaaagaagg tggcgtgcgg ccaaattggc gggattaaat gaagtccctt gtttgattgg
    11461 agattatagt gatcaacagg catgtgctct cactttaatc gaaaatattc aacgtcagga
    11521 tttgaattta atcgaggaag ccactggtta taagcgttta attgaagaat tccattatca
    11581 tcaggacgag atcgcagcgt tggtaggtaa atctcgtagc catgtcgcaa atattcttcg
    11641 tctccttacc ctgacagata aggttaaaca gttggttcga gacaaagtgt tatcctttgg
    11701 ccatgctcgt gtattggttg gcttaaatcc tgaacagcaa gaaatgtttg ctcaccagat
    11761 tattggagag gaatggtctg tcagatactt agagcaagaa attaaatctt ataaaaataa
    11821 agatacgaac agtcccaaaa atcccagaaa agacagagat attgagcggt tacagactat
    11881 cctggctgaa caagtggggg ctcctgtaca aattgtaaac gatggcgaag acggaggttg
    11941 gttaaaagta aaattttttg ataatgatac ccttgcaggg cttttggagc gattgggctt
    12001 gagatatgat tagcttattg gaactaactc agaaattgtc actatgtatc aaacaattca
    12061 aactgattta tattgtgaaa ttcaatgtta aggaaaatat ttaaatgaaa aggacgttga
    12121 ctggggcatt catgtgggca gtatggtcat tgtcatctca tgcggctagt atgcagttcg
    12181 aggtggataa attaattaat cggctaaacc ctcgtgtgaa cctggggata gtagtgaccg
    12241 atctaacctc gggcgagacg ctatataaac gcaatgccaa tcgtttgtat attcctgcca
    12301 gcaacatgaa gcttttttcc gaggcagcgg ctctgatggc cttgggtcca gactaccagt
    12361 tcaagaatca actcagtacg agcgccaatc aattacaaca aggtgttttg cacggaaact
    12421 tgtatttaca tctcagcggc gatccttcgt ttagcagaga agatttaaag gcgttgcttt
    12481 cttcactcaa ggattggaat atcacaacca ttcaaggtaa tgtgatcatt gacagtagtc
    12541 tgatgtccat tcccgcctat cccccaggct ggctaacttc tgatttatct tatagttatg
    12601 gtgcgccaat agcacctttg atggttgatt ccaaccgatt aacaatcacg gtcaaccccg
    12661 gggctaaagc aggcgctcct gctattgttg aagtagatga tggcggcggt actattaacc
    12721 tgaataatca agcgaccacg aaggcgagtg agaaaggatg tggtgtaggg ttttatttgg
    12781 atcctgagaa caatttaacg gtaagaggct gtgttggatt agggcaatgg gctgttcagc
    12841 aacgcatcgc tataaagaat ccttttgttt atgcccaggg gatgattgtg agtgagcttg
    12901 ccaagtctaa tattaaatta aatggacagg ttttgcttgg aagagcgcct gctggtactt
    12961 tattaattgc cactcgctat tcaaagccaa tttcccaact gatggcagat actttaaaac
    13021 cttctgataa tttatatgct gatagtttgt acttgcatgc tgctgccaaa attaagggta
    13081 gtcctgtaga ttggaagcaa gcacagccag taattaaaaa ttttttgcag caacaaacag
    13141 gaatcgattt aaaagactcg aattttacag atggttctgg attatcacgt tataatttgg
    13201 taactccagc gcaaaccatg gcgcttttaa aattcttgta tcaacgtttc ccattatctt
    13261 atgaatacat tgctgcttta cccatctctg gtcgggatgg tactttgcaa aaacggttta
    13321 aaaccccaaa tcaacaagga tttgtgcggg ccaaaaccgg gacgatgacg ggtatgaaca
    13381 gtttatccgg ttacttgtat actgctaatg gccatacttt ggcttttgct atgtatatca
    13441 atagattacc cggaaaaccg gctgggccag gacgtccatt attagacgca ttatgcactt
    13501 atttcttgca gcaaagccca acaagcagtc gactggcacg ggtattatct ccccacagtc
    13561 gcattaagtt ccaatttaat cctacacaaa ttgagttaca gagagttcat caagccaaat
    13621 ggaggcgttt ggaaacggca gtgaggcagg tcttgagagg acaggatgtg aatgtggttt
    13681 accggggcaa tgaattaatc gtcacagaca atcagtctaa tgcgaatagc gtttggaaag
    13741 ctttacaatc aattggcaag aaatattcat tcgccgtagc cttgtcttct aaagtgatgc
    13801 ctgttacacc atcgggaaaa cctttgcttc tttgggtgca agcaccttta tctgaaaata
    13861 aggcagagag aacctggata attcgggaag cggtataacc cgagtttgat aattaaaaaa
    13921 aagacccgcc tcgtattact aaaatgcaat gcgggactag tacaatcgtt ccataagtta
    13981 tggataagat cagtcgttat ctcgaaccgt ttgggctcag gaggcattta tgtcgtttcg
    14041 aagccttgta tgagatttaa tattttgtgc caaggcttcg agacgatgct tatgcatctc
    14101 ctcagcctga acggcccctg gatatcgact ggttctcata taatccttat ccatttattg
    14161 ccgcgctgca aacatgtctg cggtcctgat caactgattt tgaattgatt tgagctgagg
    14221 catcatcagg gattgggttt tgaatgtgct gttctgccca aaacatgatt taaataacgt
    14281 gaagttatgg ataagatcag ttgctatctc gaaccgtttg ggctgaagag gcatttatgc
    14341 cgtctcgaag ccttgtatgg gatttcatat ttcgtgtcag ggcttcgaga cgatgcttat
    14401 gcatctcctc agcctgaacg gaccctggat atcggcaggt tctcgtataa tccttatcgg
    14461 tataactcat gttaaataga ttgttatttc tgcattcggg ctttgcagta ttcaaggtga
    14521 ttgtaacagt acttacgaaa actccaaggt caaggcaaaa aatgtttata atgagggagt
    14581 ttagataaac taaatggccg aattaaaaac attttaaaca aagagattga ggtttatcgt
    14641 aagtcctgta taaaagagct ataattgaga actgaataat ttcggtataa attggtttgg
    14701 agtagtaatt gaattttagt attcgggtaa ttcttttatt aacatgtttt gtttctttag
    14761 ccttttctgc accaaaattt ctaacaatct ctgatattca ttatggtgca gaaaatatag
    14821 caaaggatgg gcaggatact ggaaaggagt ttttggatgt cacgttcaaa cgatttgagg
    14881 aattgtctca aaatgtagat tttattttac atttgggtga tctgcctact cactcgttat
    14941 tttcgacatc taaaaaagaa gaatacgaac aagttgtatt tcatggtttg catgaagcga
    15001 atccgggtca aaagcccatg ttttatatta caggaaataa tgattccttg cttggcaatt
    15061 accagccatt ttcttcagac aataagtcgc cactcaattt ggctctggat tgggatggtg
    15121 cttgcgttca ttgtgatggg ttaattattg atgatactca tatgcgcaag gatggatatt
    15181 actccagtta tgtcatcccc aataataaag agattatttt aatagcactt aattcggttc
    15241 aatggaccaa gacccctgtt tttttaccaa agtaccctaa tcaacaaagg gatgcttttg
    15301 tacaattatt ttggcttgaa caacaattaa aaaatcatcg tgcaaagcaa ttgttgttgg
    15361 cgatgcatgt tcctcctggt acagcctata acggcaaccg gttttggcat gatatttatc
    15421 tggatagatt tttgacctta ttagacaaat atcatcgatc ctatgatcaa atcaccatac
    15481 tgttctcaca ctcccatatg gaggaattca ggaaaatcaa attatccgat ggctcaacta
    15541 tttatgcgtt ttctactccc ggaataagca gggcgcatca caacaatccc gggatgaaaa
    15601 tttttgattt agacaagcaa atgagggtca gaaattttac tacttatttt acgtccgatt
    15661 tgtatgagtg gggtaatgag cattatcaag ccatgaacat tcctgacgct atttttccaa
    15721 attgtcaaaa taaaacgata ccccagtgcc tggacacctt aagtccagag caagtatgca
    15781 ataatctgga agcaggacta ttttatgggg tgaaaagtac gcgtgttccc tatcagggat
    15841 gcattaaaac ttaccagatc aattaagtta ggatttcgtt tatcaggtaa gtttaacgag
    15901 atgttcattg ctttctttag gttgtaaata cgttgcgacc gctttttgca aggcagcaat
    15961 atcatcttcg cttcttgtcg ttaccttatt tctaaagaaa tcaatcattg gattctggtg
    16021 ggtttttatt atttttgaat ggtcagggtt taaggtaaat gcagagatta acccatagat
    16081 gttttctagg tttccatgtt ggcattttat ttcaaaaata aaatgcttta ataattcacc
    16141 tttttcacgt cgttgagcat cagtggattg acacatcaat tgtgctcttg aatggatagc
    16201 attcaacatc aatacaatat cttccggaag cttctttatc ggtttatcgg ttttgaccat
    16261 ggagtctgtt gtttttgcca gagcatcagc ggtttctttg tgcctatgtt ttgcctgaag
    16321 cggtggttca aatcgaaaaa ctttatcata gtatttctct ttgaaacgct ctgctaacca
    16381 gtcatgcatt gttgcagtcg ggtgaacatc atcccaaaac atatatcctt tggctggtga
    16441 aatatgttct ttttgatcga taggattttt ctgattttcc ttaaaaagat ccgattcaac
    16501 gtaaggggat tttaacttgt ctctatcaaa gccatattgt tcgggattac tgtaaacttc
    16561 cttaaactgc tcattaacat caaagacgga gagattgaca ggaattccca attctttgta
    16621 ttttttgatt aattcaagag attttgtctg cagttgctga ttaaagtatt cggtacaatc
    16681 ggatgcatta cgctgttctt cagcattttt tctttgaaat cttggagtca atgagagatt
    16741 gggcaaattg aataaaacaa aatttctgta cccattatga ataagcgctt caatattttg
    16801 aattcggtcg ttaacagctt tctctgcagc cgcacgagaa ggcttgtcat tggcggtgat
    16861 caagtcgttc gcgcctgacc actccacaac taatgtttca gatttttcaa ggttggaaat
    16921 attgtatttt ttatcatcag ccaataattg agtccgtttt cctcccaaag tagcgagaat
    16981 caatcttgta aagaacagaa ccaggttaaa ggtaaagcta aaagagtagt catgagaagt
    17041 taatccacct tcgcaataga agcgggcaaa tctttcccct ttaaacagaa tatgattgtc
    17101 ttcatttaag ctaaaagagt tttcattttc tcttcgcgcc ttatcattga tgagtaattc
    17161 atcgcctata tccgcattgt ctcttgcatt attatgcaat cttaattttc ttcggatatg
    17221 ctctatctca aattgctctg cagtggtcgc accgacataa tccccccaaa gaaaaccatt
    17281 agtgaatcga ccttttggag atttgcttct taatccactt aaataactca tggggataaa
    17341 accgagcagc tcacgtttat taagcgttcc tctatcggat aaactatctc ccaatactac
    17401 aaggtgtgtg attttagttg gtttttgggc catgatattt gcaagaaaaa taacgtgatc
    17461 acattataca caaaatgatt tttaaatggt atgttttgga ctgctcttct ggtgaaattt
    17521 ttattgcgga ggacgcaaaa tagctgaatc aagtttgatt taggaatacc ctcacctaat
    17581 ccacttgcaa agtgaaccag gatgagggga gtttagctta accgattata ttgattttaa
    17641 gatggtgatt tgctcaggca gtcattgact cttcttctga ttcattatca gcaagagcac
    17701 ggatacgttg tgcctcaata gttaaaacct tgaagtaatc ataggacatg taaaaattac
    17761 ccttgtctcc aaattgctca ccccatgagt tacgtaaagt aaataatcct ttgtgctccc
    17821 ggccattgtc atcaacagcg acggcattgt catcatatcc agtgataatc atttcatggc
    17881 cgccaaaaaa tggacgaaga taaatatcac gagcaatttc aggggttagt acccaagtat
    17941 cgaattgtgt tttattcgtt cctacggcac ccataacacc caaatcaaaa tccagcaata
    18001 atactgcaaa agtaactcga tctttttgat tcaaagcgat ttttatatca ttaattgttt
    18061 tgttggtgtc tgttctgtct aatacagcct gataaacatc caggatgggc gtgtaaataa
    18121 caggattagt ctctggatct tcgctcattt catggaattg ctcaagcgac atggcatgat
    18181 caggggtagg ttgctcgttg tctgggtatt cagtgagtcc accacatccc aggttttttt
    18241 gcttttcttt actgactatc ccaaaatttt ccatttgact taagactatg cgccctaatg
    18301 agccatccca accacttggt gaatgaccgt ttttctccag atacagtcct agttgtaatt
    18361 ggcacaattg actgatgtag tcacctttgt ttaaagcagc atctacggcc gcagtcgctg
    18421 caaaagtgac gcaagttcca aagcttccct ggttcagaac aggtacgtta ttcataccta
    18481 attctacttt ggatgggaga gctgtcgata tggctatggt attcggtttt ttgagtgtaa
    18541 tgtctgtttt tctcgcaagt gccttgatag cagaatttga caactgcatt ttaagcaatt
    18601 tgatttgttg tatggcgggt tttgtattga gtgttttatt ttgaggggct tttaatgtct
    18661 ggtttaacgt tcctactact ttgatatctt gagcaaataa attcccgctc aatgccaaag
    18721 ttaataaaag taattttgaa acacgcatct atcattctcc gttagtgaca tggacttgaa
    18781 atggctggca aggataactg atgataaaat aatattcaat atatatttaa tatgcccact
    18841 aaagaaaaaa taaatccata ttgggtttta attgtcttct agggttggat tgagtcgttt
    18901 catccaggac atgaataagc gggtcagagt taattctgat attaactatt tattcgactg
    18961 gaaaattgaa ataggtgtct ggataaggtt cgtttttcag ggtgaaatgc caccattcat
    19021 agtaataagg tctaaaacca aagcggctca tgagtttttg taataatttg cgattggctt
    19081 tctggttttt actcagcttg gaataatcaa tgttggctga cacatccaga caatcaaagc
    19141 gagttcctgt atcgatcgaa tcgtcatcaa gatagtttgg ctttttatcg tagcaacgtg
    19201 ttaaaggatg attagatttt ttttcttgag aggctgttag tttcaccaga gttaagtcaa
    19261 gggtactgcc tcgcgtgtgg ccagaggtta gagcaatata accacggtca aacaaatctt
    19321 gttttatttc acggggataa aattcaggtt tcatgcgatt atctttggca ttacggctcc
    19381 acttataaaa ataatccaca gccctttgtg gcctgtagca atcatatact ttaagcgtat
    19441 atcctttggg tttgatgtat tcttccgctt tttttaattg ttgcgcggtt tgtctggtga
    19501 ctatacatac tccgcgtttg taacctggga taggatttcc tatgaaatta ttatgggtgg
    19561 catatctgat gtcttcaatg atatcaggcg ctacatcgtg taaataaacg aagcctttgg
    19621 gaagtgaaaa actggccatg cttatgagag aaagaaaaat aagagcgatg atttttttca
    19681 ttgttagatt gttaaactca taacccttag aaatttactg gcgtgggggt gctttcgctt
    19741 cgctcatcat cctccatcgc tggtgttaag tcaactttcg aggtcatatc tttgggtttt
    19801 tcaaactctt gttttggagt ggattgtctt tccaagacag actctcgcgt cacacctaat
    19861 tgttgatgaa gtttggcatt caccgttaaa ttatcctttt gttcggtttt gtgttgtggt
    19921 gaattttctg atgccgcttt cataccctcg cgaacaatgg atttaatctt tccttcttct
    19981 atcttgttat taatggcatc aatagcggta ccaaaaagat aagtggccgc aaaagtaacg
    20041 ccagcaacaa tggcagcacc ggctgccaca ccaattccca atgcggccac agccaagcct
    20101 gccgggggga atagtaaagt aagggcgact aacccaccag ccgctagcaa tccaagcccg
    20161 aggcccgctt taatttttgt acccatattt ttggtaaaag cgttttcagg ccccatttct
    20221 tttactttgg caaaattagc gatcttaccg aaaaggccat tatcagcgaa attggcttta
    20281 tcctgaattt caaactttcc gccggaaaca cccgtattgg cagtatgatg aagggcctgt
    20341 atggtagtaa gttcccgatc aagttgttct actttactga cttctttacc atgtgtcctt
    20401 cgatgatcca gaaattcacc agtgaataac ccgtcctcac tcattgctgt ccataaagca
    20461 ttgccaacaa ttttttgcgc caatctttgg ccacgatcat tattactctt acatcctttc
    20521 gtattggcgt aatccagctc gcaggagaga gaagccatta aggtttgcag cttgaaatta
    20581 tttttgccat gatgccaggt gttctcataa tagagctctt gagcttgcat gaggtaagcg
    20641 catgagttgt aaaagtgctg cagtttggat ttggtttcag gatctgtttc tttattctgg
    20701 attacagggc ttatttgttc tttaagttct tgtaaagcct tattgatttg ccctttgtgt
    20761 tctgtgtaat aagcctttct taaggcatcg atttgattat gcagttcata aattgctgat
    20821 tcttgttttt taatgctttt ttcatagcca tcaacctgat ttttcaattg atccagcttg
    20881 gcttgatccc atttcccttc gcctttctga tattcttcga aatagctgaa taatgcttct
    20941 cgataggctt ctttcacctc ttttaagtca ttttctttcc tggcgatttg ttgattcaga
    21001 ttgctgacgt ctgggccttt taataacgta tgtaatgcgc ctgctttgtc gtaatcaccc
    21061 aggctttgta ccgctttgtc taaattgctg atgacgtcat cagtcatttg atttacaaaa
    21121 cgcgcatttt ggttttttgt gacactattg tcagagagcg gattgagcgc tccaacctgt
    21181 ctaaaccaat tcactcccca ggaactcata cgcgcttgat aaccaacctc tgtttccact
    21241 tctgtattgg agccggtttg aatggtgatt tttgctttgg agccatcaaa agcatccatc
    21301 atttgccgta cgtattcaaa ttgttctttt tgattttcgg ccgctaccct ttgcccggct
    21361 gttacagctt gtgtataaca ttccgtgaca gataatggat tatgttctac atacagcttt
    21421 attaattgtg ctcgtgcttt ctccgcatca ggaggaagct catcagggcg atactcttca
    21481 ggaattttat tccaaaggtc ttcattggtc attcggtcaa tttgacgctg tgccagggtt
    21541 tggatgactt cttcctggtt gattaaggtg accgctttga tagccagctt tttatagtca
    21601 tcagcaccct taaaatcaga atagggcatg cgtgcgccag gaccagaaaa actgtcatgg
    21661 tgaatggtca cggtgccatc agaatgcctt gtcccggcaa tcatgcgcgc catactgctg
    21721 ttgcccagaa ctaggtcaga gcgtttatgg cttgtagtgt cgtgatctcg gttgacttcg
    21781 gattcatcct tgcccattag tctttgttgg cgtttggtgt accttccaca tggaatgtac
    21841 ataataaatt gttcttcgcc ttctttagag ttgggcgctt taaagcgatg tatcaaggta
    21901 tcgcgattat gactgcgaat attatgccct tctttatcca gtgcttttaa tttatgacct
    21961 gaggcttttg cgacagcctg tgtaaattgc tgtaaactgc cttcgacttt ggccaactgt
    22021 tccagaaagt ctttattagc agaacttttt ttacttgaaa acaaatctgc ttttgctttg
    22081 tcggaaggaa tggtttctga aatggacttt ttgaaagttt ttgccatttc atcaatggta
    22141 ttctttctcg aatccttctt gtctttgtta tctttgtcta tcagagattt ggcagcggct
    22201 acaagattgt ctatataatt attcgcctct tggatcacat caggttgccc tgtaacaggc
    22261 ttgccatgtt tatcatattg actgttcacc accacgactt gtttcccgtc cagcatcggg
    22321 tctttgaata cctgtctttc gccgggataa ccgtaggcaa gatgctgact ttgagaagga
    22381 acgggttcca gatcacttaa taaatgcgtg acataggttt ctatattttc agtgattcgt
    22441 ggcatggttt attccatatc ataacattta ttttattata gctggcaaac attaacgtaa
    22501 gattaataag ttcttgcatt gagtgattgc gtagggcttc acttaaagac tggactgaaa
    22561 aatagaattt agtactattc tcagaaaata gtgaagcctg ataatttcct agtaaaactc
    22621 aatagtggtg gtagtcacat ccaaaggctc cggatcatcg acatctaaaa tgacaggctc
    22681 aaccactgga gcggcaactg tcacttgttg gtattcaact acttctgctg tagtacaaca
    22741 gcaacctgca gttgttataa taacaacgat cagcattatc catttcatag cgaaacctct
    22801 cgtcataact taaccaaatt gtagattatt tgttcatata ttgccaatgt tttatggatg
    22861 cctttgcgtg tttgttcatc tgagcaagtt caaattcgtt tgaaatgggt ttattctttg
    22921 gagtgctttc cattaatgta ataaaggaat gccaaaattt gggctacggc aatataaagt
    22981 gctttgggta tttcctcatt gaggcgaact tgagataaga gcgctgttaa ttcgggattt
    23041 tgttcgagag gaatgccatg ttctttggct gtattgataa tttgctgagc aaataaacct
    23101 tctcctttag cggttacttt gggaggagaa ttgccatcat aacgtaatgc aacagcgacg
    23161 ggtttctttc ttttcatatt tttatatcca ataaacgaag gtttgtggca tcgatatgat
    23221 tttcttccaa cccaacttgc agcttccatt ctcttaagtg aagacctgat tcatagagcg
    23281 tttcttccaa atccttatgg taatcattga ggagttgcaa tgttgacgat tgctgagtat
    23341 taaccttgat atcgatgttt ttagcgtaaa tcgaaaccgt agcttgtatg cttcctaaat
    23401 gggataaatt caaagcgaac gatattgacc atttagacag ttgcataggt tctgccgtgt
    23461 gttgtttgat cataataggg attacatcaa tgccatcggg ggtttttaaa ggaaaatcaa
    23521 gcatgattag ataggcttct tgattctcct gcgataaata attaatttga tttgtcgtta
    23581 ttcgcgctaa tgcctgtgtt acctgctcac gtaaaatatg gtgtaccgcc tctgttgata
    23641 gtccaataag attcaatatc gtgtctttag tcagtggctg agggatagct cctggtaaag
    23701 gaaggggcgc ttgctgaaga aaacctgatt ccaattcgtt cggcagattg attgggagtt
    23761 tactgtctgt acctaaactg tgtaataatt taaaacacaa ccctttaaaa tccgattgta
    23821 tttctcgggg tatctttcct tttaacaatt tggattctaa gaacacacca ctttgactaa
    23881 tcgcttgcat aagttgatta ggtaattgag tcaaaacagg gacattgcct aaaatggctt
    23941 tcataacctg attgatggga gccgataaat tattttctgc catcaattga gtcagtgtct
    24001 gtaaaagatt ggtagctggt gcttgttttg gtaaatactg aagtaacgca ttttgcaata
    24061 gagatggaag cagtgttttt tgttgcactt caaggatggt ttcggggtgg ttcgctatga
    24121 cttttacttc aaataactct cccggtgtaa aatgatgaga tgcctttgcg ttgagatttt
    24181 gcccattgat attgataaga acctgatcat tagacagtgt tgtgacaaca accgttttca
    24241 gaatttgtcc tacataaagt tctgtcgccg gttttgctaa tttgatttct tgtccaggag
    24301 ggaatctgac actggaggtt ttgctcatct cgaccgacat acaccatcct tatcatcagt
    24361 tagactgctt tttcttatcg tcgtcccccg ctggactgtt tcgtaagttg tctaataagt
    24421 atatactcat cgtcttaatt atcggcagaa ttatgaattt ctttatagtt cgttgggaaa
    24481 ggataccagg catggatata agagtaatca ttacacaaaa tgtaattgaa atagccttat
    24541 tcatctggtc attgcgcaat ttaaacaggg tgtggtgatg ttagatagag caagtgtagt
    24601 ggatgaacaa tttctgaaaa gagtgctgca aggggatttc ccacaattaa gaagcagcat
    24661 gagccccgat acagcagagt tggacaagaa aactgctata gaattttttg actcacaaat
    24721 taaatctcgg cttcttgata ttattgcgag acaattaaag gaaaaaggcc tctcttttta
    24781 tacgatagga agtagcggcc atgaaggcaa tgcggtcttg ggtcaggttt ttagagcgag
    24841 tgatatggcg tttttgcact atcgcagcgg tgcctttttc ttgcaacgag caaagcaatt
    24901 acctggaatc gatggagtaa aggatatctt gttgtcatta gtggctgcgg caaatgatcc
    24961 tatctcaggg ggcaggcata aagtctttgg cagcgtccct ttaaatattc cgcctcaaac
    25021 gtcgactatc gcatctcatt tgccaaaagc tttaggtgct gctttatcaa ttacccgagc
    25081 gagagagtta ggattaccaa gtaaactggc gcctgattcc gtgatacttt gttcctttgg
    25141 tgatgcttca accaaccatg caacgagcca agccacattg aatgcttgct cttggataac
    25201 ccagcaaaat tatccgctac cgattgtgtt tatttgtgaa gataatggaa tcggtatttc
    25261 agtgcctact ccatccagtt ggattaagtc atctgtctcc tcgcgtccag gaatccatta
    25321 cattgagtgt gatggattaa acattgctga tgtttttgcc aaagcgcagg aagcggacta
    25381 tttggcaaga gtaaaaaaac aacccgtgtt cttgcacatg cgttgtgttc gtttgttggg
    25441 ccatgccggt tctgatattg aatcgcaata caacactcag gaagaaattg agcaacgaga
    25501 agcaaatgac cctttattgc atacagcagg aattttatac agagagggtt ggatgagttt
    25561 gcaggcaatg gttgagcttt atcaggacaa ccgagcttta attgaagcaa aagcactgga
    25621 ggcaatcaga gaacccagaa tgagttctgc tgaagaaata atggcttcaa ttgttccacg
    25681 agttgagaaa aagccaaagt acccattgcc agatgataat agacgagagc aagtgtttgc
    25741 cggagcttat aatcaactca ccctaaagcg taatttatgc cagcagatca attttgcttt
    25801 gaccgatctg atgatgcagt atccaaatat gttgattttt ggagaagacg taggaaagaa
    25861 aggtggagtt tatcgagtta cggctgattt acaggctcgt tttgggcaac gcagggtttt
    25921 tgataccctt ctcgatgaaa cgaccattat aggtaccgct attggtttgg cacataatgg
    25981 ctttattcct gttccggaaa ttcagttctt agcttatctg cataatgccg aagatcaatt
    26041 acgtggagaa gcatccactt tatctttttt ttcaagtggc caatatcaaa atcctatggt
    26101 acttcgaatc gcctctcttg cttatcagaa gggatttggc ggtcattttc ataacgataa
    26161 ttcgatagcc gttttacgcg atttgccagg ggttatagtg gcctgccctt ctaatggccc
    26221 ggacgcagca aaaatgctga gaacatgtat gcgccttgcc tatgaggaag gacgggttgt
    26281 cgtgttttta gaacccatcg ccctctacat gaccaaggat ttgtatagcc cgggagataa
    26341 tgggtggtta tttgaatacc cgtcacctga tgaaatgatt tctcaaggag aggttggcgt
    26401 atatggggag ggggatactg ttattttaac atatgccaat ggatattatt tatctcgcca
    26461 ggcggaaaag gtcttgcgtg aagtacataa tatttctgtt aagatcattg atttgcgctg
    26521 gctcagcccc ttgccaaaag acgccatttt aaaagaaatt gccaaagcaa aacgtatttt
    26581 aattgttgat gagggtagac aaagcggctc aattagtgaa ggattgatga cattattgat
    26641 ggaagaggct tcgccacggt taaaaataaa gcggattacg ggcaaagatt gcttcatacc
    26701 attaggaact gcttggcagt atttattgcc aagtcaggaa agtattatag acgcggtaat
    26761 cgcattacag tcagataaaa gggagaagga aagtggacga cttgttgttt cttgatgaac
    26821 aattacatga tgacgagcgc atgatacgag atagtgtgtc tcgttttgtt agcaatgacg
    26881 taataccttt aatggctgaa tcttatgaac atgcccattt tccaaaagag ttgataaaaa
    26941 aatctgctga tctgggtttg ttgggcttaa ctttacctgc ggaatatggg ggtgcagaag
    27001 cttcttatgt tgcctatggt ttagtttgcc aggaattgga gcgaggtgat agcggtttgc
    27061 gcagctttgt ttctgtgcaa agttctttgt gtatgtatcc tatttttaaa tttggcagtg
    27121 aagagcaaaa aaaacgctac ctgccagaga tggctgcggg gaatattata ggttgctttg
    27181 gattaactga gccagactca ggatctgacc cggccagcat gcgtactcat gccaaaaaaa
    27241 ttgaaggtgg ctggattttg aatggcgcta aaatgtggat taccaatgcc cctatcgcag
    27301 acatcgcaat agtgtgggca aaaacagagg caggaattcg tggttttatt gtcgatacaa
    27361 aatctgaagg catgagttgt cctgaaatta agttaaagat gtctttacga gcttccatta
    27421 ccggggagct ggtctttgat aatgtttttg ttccagacga gaatttattg ccgaaaagtg
    27481 aaaaagggct tggagctgcg ttaagttgct tgagtcaggc gcgttatggt atcgcatggg
    27541 gagcaatggg cgctgccatg gcttgctttg atatcgctcg ggattatttg ctggaacgca
    27601 agcagtttgc caaacctttg gcctcgtttc agttgattca aaaagatttg gctgatatgt
    27661 atacagaaat tattaaagca caatgcctta atttgcagat tgggcgttta aaggatcagc
    27721 atcgtgagac accgactatg atttctctgg ctaagggtaa tgcgtgccgt gaagcattga
    27781 aaattgcgag aaaatgtagg aatctaatgg gcgggaatgg aattagtctt gagtatcatg
    27841 tcattcggca tatgctgaat ctggagtcgg tcttcactta tgaaggtacc gataatgtgc
    27901 atactttggt cttgggaaga catataacgg gaattaatgc ttttggttaa ggatttttct
    27961 gcttttatcg agttagttct gaataccaca agataactat tcttaatcag taatgattac
    28021 ggttcaagtt cctttttctt atggaaagag gaactgctta aacctttttg attttagagt
    28081 tgagcgattg cccgccagat tatggtgctg aattaggaag gtatgctcct ggcatgggtt
    28141 attttatgga tattcgatgt aaaacggttt tatacaaaca attacaggag tttaatatga
    28201 ttaacatgaa gttaaaagca actttgatag cctgtttgtc cgttctaact ttaagcagtt
    28261 atgcgaattc atcagaaaat aaagaagcca ttttacaaca gtgtcatgac ttggcttcaa
    28321 cagtagcctc tttggtttcc agccaggcta aaaaaacctg tgcggaaaaa ttggttattg
    28381 cttcattaca cattgataca gcggcggact ggattgtaga ggatgttcat tctgcagcaa
    28441 aacaagaatt agataatgcc atttattctt tgcaatatgc tgagctgaat agttgtaatc
    28501 gctatataca aatatctcat tccaaactgg aa
//
LOCUS       DAXLDT010000028        27519 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXLDT010000028 DAXLDT010000000
VERSION     DAXLDT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27519
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.028"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(159..2426)
                     /gene="clpA"
                     /locus_tag="ACF2SP_000956"
     CDS             complement(159..2426)
                     /gene="clpA"
                     /locus_tag="ACF2SP_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFD2358758.1"
                     /translation="MLNKELEFTLNLAFKEAKEKRHEFMTVEHLLLSLLDNPAAGNVL
                     QACDANIEALRRDLIEFIDETTPRIPEDELDRETQPTLGFQRVLQRAVFHVQSAGKTE
                     VTGANVLAAIFSEQESQAVYFLRRENITRLDVINYISHGVSKYQNNDMHENMNSSMEE
                     DMMTPEGNESPLDSYCTNLNRRAKQGKIDPLIGRHEEIQRTIQVLCRRRKNNPLLVGE
                     AGVGKTAIAEGLARRIVDGEIPDSIRGCVVYSLDLGALLAGTKYRGDFEKRLKAVLKQ
                     LGQQEGAILFIDEIHTIIGAGAASGGVMDASNLIKPLLANGELKCIGSTTYQEYRGIF
                     EKDRALARRFQKIDISEPTIDETFEILKGLKGKLEAHHGVKFSIPSLKAAAELSAKYI
                     NDRFLPDKAIDVVDEAGAYQNLLTANKRKKIISVTEIESVVAKIARIPIKKVSARDKD
                     TLRNLERDLKLLVYGQDQAITALASAIKLGRSGLREPQKPVGCFLFAGPTGVGKTEVT
                     RQLANVLGIELLRFDMSEYMEKHTVSRLIGAPPGYVGYDQGGLLTEAVTKNPHAVLLL
                     DEIEKAHPDVFNLLLQIMDHGTLTDTNGRQADFRHIILVMTSNAGASEIVRNSIGFST
                     QDNTNDGLEVIKKQFSPEFRNRLDAIINFAPLDTQTIGLVVDKFIMELDEQLSHKGVT
                     FTVDKSAREWLMEHGYDKAMGARPMARLIQENIKKPLADELLFGKLMHGGHVTLRVKD
                     GKLHFDSHDHREGVC"
     gene            complement(2458..2793)
                     /gene="clpS"
                     /locus_tag="ACF2SP_000957"
     CDS             complement(2458..2793)
                     /gene="clpS"
                     /locus_tag="ACF2SP_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFD2358759.1"
                     /translation="MSKQNLEEIIQTGIADTELSTEISTAIKRPRKYKVLLLNDDYTP
                     MDFVVEVLKHFFHLNEEVAIQVMLQVHFQGKGVCGVFTRDIAETKVALVNEYARMNQH
                     PLLSSMEPE"
     gene            complement(3195..4463)
                     /gene="icd"
                     /locus_tag="ACF2SP_000958"
     CDS             complement(3195..4463)
                     /gene="icd"
                     /locus_tag="ACF2SP_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFD2358760.1"
                     /translation="MESSMTYDKIKVPAQGEAITVAADHSLHVPDNPIIPFIEGDGIG
                     VDVTPPMIRVVDAAVQKAYGNKRKISWMEVYAGEKATKVYGGDQWLPKETLDAMKKYV
                     VSIKGPLTTPVGGGIRSLNVAIRQDMDLYVCLRPIRYFNGVPSPVREPWKTDMVIFRE
                     NSEDIYAGIEWQADTPEAKKVIQFLTKEMGVKKIRFPEHCGIGVKPVSREGTTRLVKA
                     AIQYAIDNDRSTVTLVHKGNIMKFTEGAFKDWGYQVARDSFGAKEYQGGPWMEFKNPK
                     TGKQIIINDVIADAFLQQILLRPEDYSVIATLNLNGDYISDALAAQVGGIGIAPGANI
                     SDQMAVFEATHGTAPKYAGQNKVNPGSIILSAEMMLRHMGWYEAADLIIRGMEGAIEA
                     KTVTYDFERGMQGATLVSSSGFADAMIKHM"
     gene            complement(4561..5259)
                     /locus_tag="ACF2SP_000959"
     CDS             complement(4561..5259)
                     /locus_tag="ACF2SP_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFD2358761.1"
                     /translation="MKWVLVVMAIIAQTSCAVNHRVMVGDEPVIIQQTKGQGKTFVHV
                     HHNEQTALKAAKAVIRKEGGSLITLIHSGGRNIVFHLHNQRYEFDPNRIFTDKGIKKT
                     LTQYSYYTPEAHQEVKKLADKIKVLLPEGKVIAVHNNSSYSLKDYLPGHELAKDAKAL
                     YMNPDNYFRNFYLVTKLSDYLRLKMQGFNGVLQKPSATDDGSLSVYLAKRDYINVEAG
                     YDQLAEQIKMLLHA"
     gene            5520..6122
                     /locus_tag="ACF2SP_000960"
     CDS             5520..6122
                     /locus_tag="ACF2SP_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFD2358762.1"
                     /translation="MPQLILFNKPYGILTQFTGELPEQTLSAFIKLPGFYAAGRLDKQ
                     SEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPA
                     EARLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIRYQ
                     IGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK"
     gene            complement(6058..6537)
                     /gene="mreD"
                     /locus_tag="ACF2SP_000961"
     CDS             complement(6058..6537)
                     /gene="mreD"
                     /locus_tag="ACF2SP_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFD2358763.1"
                     /translation="MSVLHIRLGLGFLVALALSILPMPELISIFRPPWVLLLVLYIEY
                     YLPGKFKLTALLIVGLLLDVLLSTVIGEHSFALLLVTWIASTKSRRVQFFSLIQQVCL
                     IGFFCFLYQSIIIFIDALLGFNYNLIMPVTSAILGMIIWPWIRVLGYSSLLTRSVYR"
     gene            complement(6534..7442)
                     /gene="mreC"
                     /locus_tag="ACF2SP_000962"
     CDS             complement(6534..7442)
                     /gene="mreC"
                     /locus_tag="ACF2SP_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFD2358764.1"
                     /translation="MISSQQNNKHNRLFAKGGHSSLGFVFALAFSILLMFSDYHYRYL
                     DFVRSGFSLIVSPLQYAVDYPVRVIGWIQSLVSAKKALIDENMRLKYKQTMLEAELQK
                     LIVIQRENSQLKELLLTSSKADMRVMAAQILAVDTSQARQVVVLNKGTRDGVYVGQPV
                     LDAKGVMGQVIDVGPMTSTLLLISDSKSAVPVRNNRTGERAILVGTNDIEELSLINLP
                     KTSSIHPGDVLVTSGLGRRYPEGYPVGRVEEVKSIPGEDFVKVTVSPVALLNRNRLVL
                     LIWADSEQEELTAQINERLNAEESTA"
     gene            complement(7420..8457)
                     /locus_tag="ACF2SP_000963"
     CDS             complement(7420..8457)
                     /locus_tag="ACF2SP_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFD2358765.1"
                     /translation="MFRKLRGVFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVALRNE
                     SGQKRVAAVGLEAKRMLGRTPGNINAIRPMKDGVIADFFVTEKMLQHFIHKVHENKFL
                     RPSPRVLVCVPCGSTQVERRAIRESAMGAGAREVFLIEEPMAAALGSGMPVEEASGSM
                     VVDIGGGTTEVAIISLSGIVYHQSVRIGGDKFDDAIVSYVRRNYGTLIGETTAERIKH
                     EIGSAFPSRDLFEIEVRGRNLAEGVPRSFTLTSAEILEALQEPLSGIVGAVRAALELA
                     PPELAADIAERGMVLTGGGALLKNIDTLLMEETGLPVLIAEDPLTCVARGGGKALETM
                     DLRGGDFLSTE"
     gene            complement(8653..8970)
                     /locus_tag="ACF2SP_000964"
     CDS             complement(8653..8970)
                     /locus_tag="ACF2SP_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFD2358766.1"
                     /translation="MKARLIAILLSFIVIPFSSQAENEPQKTPAKQVLVKGKINLNTA
                     DVYSLTGSFKGIGKKRAEAIIAYRENHQGFKSLEELAEVKGIGQRFVDKNREKLKEIF
                     VIN"
     gene            complement(9062..10477)
                     /locus_tag="ACF2SP_000965"
     CDS             complement(9062..10477)
                     /locus_tag="ACF2SP_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFD2358767.1"
                     /translation="MFKPQGLYDYICQQWQEEILPSLCDYIKIPNKSPHFDAKWEEHG
                     YMEQAVNHIANWCKSHAPKGMTLEIVRLKNRTPLLFMEIPGQIDDTVLLYGHLDKQPE
                     MSGWSDDLHPWKPVLKNGLLYGRGGADDGYSAYASLTAIRALEQQGLPYPRCILIIEA
                     CEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELI
                     NEGVHSGSASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEI
                     LGEQVYSEFPWIDSAKPVIQDKQQLILNRTWRPALTVTGADGFPAIADAGNVMRPVTS
                     LKLSMRLPPLVDPEAASVAMEKALTQNPPYNAKVDFKIQNGGSKGWNAPLLSDWLAKA
                     ASEASMTYYDKPAAYMGEGGTIPFMSMLGEQFPKAQFMITGVLGPHSNAHGPNEFLHL
                     DMVKKLTSCVSYVLYSFSQKK"
     gene            complement(10452..11543)
                     /locus_tag="ACF2SP_000966"
     CDS             complement(10452..11543)
                     /locus_tag="ACF2SP_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFD2358768.1"
                     /translation="MSPSIVFTGGGTAGHVTPNIALIKEFRKEGWNVEYIGSVSGIEK
                     EMIEPLDIPFHGVSSGKLRRYFSLKNLLDPFKIVLGIIQSSLLFYKIKPDVVFSKGGF
                     VAFPVVVGAWLNRIPVVAHESDMSPGLANRLSFPFVNKICLTFDAGKKYFKRQDKIEV
                     TGTPIRQQLLTGNRMKGLELCGFNSSKPCLLVVGGSLGAGSINSCIRSALKQLTSEFQ
                     VIHLCGKGKLDSSLVGVEGYCQFEYANEELADLFAASSVVISRAGANSLYEILALGKP
                     HILIPISSQVSRGDQIQNARYFQGLGISVVIQDELLKADVLLQAVQDVMRKKDEIDNK
                     IKALKIESATDKIVAIIKEQAHVQTPRIV"
     gene            complement(11540..12382)
                     /gene="nadC"
                     /locus_tag="ACF2SP_000967"
     CDS             complement(11540..12382)
                     /gene="nadC"
                     /locus_tag="ACF2SP_000967"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFD2358769.1"
                     /translation="MNNVLSQVTFDVKRALQEDVGNGDVTAALLPQQLIVEAEIISRE
                     PMLVCGQPWVNEVFKQVDNTVEMEWRVSEGDFLSTPATLCIIHGTASSILTAERTALN
                     FLQTLSATATQTYHYVQKLKGTKAKLLDTRKTIPGLRLAQKYAVYCGGGLNHRMGLYD
                     AFLIKENHIKACGSVAHAISLARKTDKHLLVEIEVETLEELQEALDAHPDRILLDNFT
                     QDMLEQAVKMNHPKYCELEASGGININNIADIAQTGVDFISVGSITKSIQAIDLSLLI
                     RETL"
     gene            complement(12379..13818)
                     /locus_tag="ACF2SP_000968"
     CDS             complement(12379..13818)
                     /locus_tag="ACF2SP_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFD2358770.1"
                     /translation="MSNSKWWFLLVCFIPAITFASEGGEHSDPVTSVLLGVTTILFLA
                     IIGRYLARLLNQPGVLGELLIGMIVGNLSYFFGSELVVILREGSAIYDVVKEMLAGIS
                     LNQAVTHVIHNSYYANQVIHALSGAEGTEYLKIAYVVDIFSRYGVIFLLFMVGLETSL
                     SDLKKTGKESILVAVIGIVAPMILGFVVACFIIPNSNYKINLFIGATLSATSIGITAS
                     VLKEMNKLSTREARTILGAATLDDILGLIILAVVSSIVISGAVSIMVVMKIIILALLF
                     FIGTLFMGPFVLRKAVDLFRFLEPWESKLFISFLFIMALAWLASFIQLAAIIGAFAAG
                     VILHDDYFGEQKAHHKDHRSIHHLVSPLETILAPMFFTLIGIQVKLETFYDWNVIVLA
                     GGLSVAAILGKLLSGLGANRRDDRLLIGIGMLPRGEVGLVFASIGRTIGVISDNLFTA
                     IVLMIMITTFIAPPLLKMRYAKPKEDRNA"
     gene            complement(14060..16447)
                     /gene="ppsA"
                     /locus_tag="ACF2SP_000969"
     CDS             complement(14060..16447)
                     /gene="ppsA"
                     /locus_tag="ACF2SP_000969"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFD2358771.1"
                     /translation="MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPG
                     GFATTADSFREFLSQNDLDKKIYDKLTALDTDDVCQLAVVGKQIREMIIDTPFTPEFE
                     QSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
                     QVYASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVV
                     FITSSYGLGEMVVQGAVNPDEFYVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLER
                     RVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEWAKDGVNGKLYILQA
                     RPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDV
                     LISDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDV
                     VTVSCAEGDTGFVYSGLLPYEQERLDVETMPELPMKVMLNVGNPERAFAFQSIPNSGV
                     GLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIERLKEGIA
                     TIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECF
                     ALECKAVRRVREEMGLDNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCE
                     LPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGLVASQFDERNDAVKALLHMAI
                     SACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN"
     gene            16672..17670
                     /locus_tag="ACF2SP_000970"
     CDS             16672..17670
                     /locus_tag="ACF2SP_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFD2358772.1"
                     /translation="MFKRIGLISIALSLNTISFTSEACSALVFNKNKPATVAVNLDWK
                     YREGAFVIHPRNAMMVSSVDYHSMHPLIWKSQYGSVIFHGGNRFKAGPSADGMNEKGL
                     TASILMLDSSIYPSNSELPALKTTEWVQYILDNFQTVQEVIDDSSNYQLVADTYRGIT
                     MNVHLMVSDAEGKSAVFEYLDGKLVIHTQDNLTTPVLTNTDYKLSLALLEEYQDHGGS
                     KALPGGYDSKSRFVRAAHYLKKLPSFIAKEEHIAYAFNGLNVVTQAPGTSSPTQLSMV
                     FDIPTKTIYFRSINEAALRVIPMDSIDFDYLYQPIALNVYQHLSGDVTDKFQPMIG"
     gene            17900..19603
                     /locus_tag="ACF2SP_000971"
     CDS             17900..19603
                     /locus_tag="ACF2SP_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFD2358773.1"
                     /translation="MSIKNEYETARDHFRLWDKQLKDNILLEKSCLMHTYKTYFPDEP
                     GFIQQLSQFSKAVAEELEPTVMENNLDANLPKIEQYNAIGQRDDRVIHHPAYVRSGDI
                     IYGSNLMQYLLKPGQMKKTLSLFLLSSHAGEAGHNCPIACSAGVIRVLSRYSQLEKTS
                     YYLDKLTHPSFSYNFTGAQFLTEIQGGSDVGTNATRANLDKQNQWRISGEKWFCSNAN
                     ADLILMTARFDEETPGTKGLGLFLVPGRLDDGSPNHYYLRRLKQKIGTRTMATAEIDF
                     EGALAYPMGDVAEGIHLVMENVLHLSRIFNSFSVLGMSRRAYQIACYYAKNRRAFNHP
                     IIEYPLVKESLAKIKSENLAMLASVFHMARCQDELDALPEPQQTKSQQLLLRTLANIN
                     KYFTAKRSVDNIHHCLDLLAGNGTIENFSSLPRLLRDCIVCENWEGTHFTLWMQTLRD
                     MHKFAIDELFIAHLQQLLEQVQDINPHKALFFGKINELDTTIKTMKSANHEEQSLHIK
                     TIIEHMASLLAALCLAIEIQQGQSPETKQAGLTLFIHEHMASCLLQHDDYMKILNKII
                     G"
     gene            complement(19806..21509)
                     /locus_tag="ACF2SP_000972"
     CDS             complement(19806..21509)
                     /locus_tag="ACF2SP_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFD2358774.1"
                     /translation="MNTITESYRAGLFQKKYWLQNIISGLIVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGLIVSIMGGSRVQIAGPTGAFIVVLSGITAQYGISGLQIATLMAG
                     FILVLFGFARLGSIIKFIPSPVIIGFTSGIAVVIWVGQWQYFFGLPAAGSGHFHEKFW
                     HLLLSFPHMNIATTFLGICALFIVLYSSKLPGLKRIPGPLIALVLVTIIQSVWHFEGV
                     ATIGSLFGGIPQGLPAFTWPEITMGRLIELIGPAFAIAMLGAIESLLSAVVADGMSGT
                     RHNSNRELVGQGIANIVAPLFGGFAATGAIARTATNIRNGGNSPLSGIIHSITLILVL
                     LFLAPLAVDVPLAALAAILFVVSWNMSEARHFIKLIRVAPRADVVILLVTFCLTVFVD
                     LVVAVNVGVIIAVLHFLRRMVNSIQIQRMSEGQLSQELAQEKNYDLPEGVMVYVIEGP
                     FFFGAVETFQSALANTHTDPKVLIIRMRWVPFIDVTGLQAMEEIILNLQKRGVRVLLS
                     EANPLVDAKLRKMGIVQLIGEQNYYKEFSQALDVCRINDSDRKSQPPFEVAKPLASVT
                     Q"
     gene            21662..23032
                     /gene="purB"
                     /locus_tag="ACF2SP_000973"
     CDS             21662..23032
                     /gene="purB"
                     /locus_tag="ACF2SP_000973"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFD2358775.1"
                     /translation="MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWF
                     ESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQD
                     KFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
                     HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHV
                     AAYPEVDWRKHCANFVTSLGLSFNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIW
                     SYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQ
                     RDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTV
                     MRRYNEPNAYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQ
                     LVKAFS"
     gene            23029..24678
                     /gene="nadB"
                     /locus_tag="ACF2SP_000974"
     CDS             23029..24678
                     /gene="nadB"
                     /locus_tag="ACF2SP_000974"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFD2358776.1"
                     /translation="MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPKLNIALI
                     SKAESIECNSRYAQGGIAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDII
                     KQLDQYKVQFKRENDGRFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
                     FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQHNRIHTFLAKCVILATGGAGKTYRY
                     TTNPMVATGDGVAMAYRAGARVGNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKN
                     PETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQ
                     IYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGAN
                     RLASNSLLEALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRG
                     LRGEMTSYAGIVRTEAGLEDLLQLILKRKKIIEEYYWKHCITRDFIELRNIILNAELI
                     VRAALSRRESRGGHFREDFPNKNFKAQESIAKLGQADSSLG"
     gene            24753..26096
                     /gene="nadA"
                     /locus_tag="ACF2SP_000975"
     CDS             24753..26096
                     /gene="nadA"
                     /locus_tag="ACF2SP_000975"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFD2358777.1"
                     /translation="MFKNSTTYQPEATLMAIRNDMAICQSDYPLDWYQEDFIPYAQEY
                     QALPDRKLTTVLNWMKPYLAKAQNHFGNKLLLLAHYYMGGEIVRLIEQFGGKIGDSYQ
                     LALMAANNPEKSVIVESAVHFMAESISILANENQHVYITNPKSGCTMEMLAKDFMVEP
                     AFLDLNERYGAENILPVCYMNTSGRVKAMTGAQGGAVCTSSNVKKIFQWAQKQNKKIL
                     FIPDQHMGENVAYWLGIKNLAYWPGGTAGANYSLPAQDKKTLKQFDKAELILFASQCA
                     VHTHYQPEMCDYWKSLGYTTIVHPECRNDVIKVADYSGSTAFIWDLVVHDKAGTKSYA
                     IGTENHMVENLKQYCNNLGIRVVNLAEAPQKDTEKGVGCGCATMSRNDPPHLVALLDL
                     LRQGKSMPYNEVKAGDVVNEFTGTRNRLPVNDQQWVINNAKKALEMMINITEDRV"
     gene            complement(26200..26586)
                     /locus_tag="ACF2SP_000976"
     CDS             complement(26200..26586)
                     /locus_tag="ACF2SP_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3757 domain-containing protein"
                     /protein_id="HFD2358778.1"
                     /translation="MKRYYSTLLVLALAHTSLSAYAHTCPDPQTTSLQWGVPPSPWVE
                     NPFSPNSPQGEENTRFVRANILVAGYGQGVTCTYRNSVGDFSILWQVRTKIPARVDYS
                     WIETMGGYVCTRGLNECQFYVADPSA"
     gene            26769..26855
                     /locus_tag="ACF2SP_000977"
     tRNA            26769..26855
                     /locus_tag="ACF2SP_000977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26803..26805,aa:Leu,seq:caa)
     gene            complement(26866..27516)
                     /locus_tag="ACF2SP_000978"
     CDS             complement(26866..27516)
                     /locus_tag="ACF2SP_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0796 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358779.1"
                     /translation="MFKKLTNILKGTNANKNIVIPYNQNGSLLLIQKEVLLNELFPFL
                     DLKSLLRVRTLSRELFDLVNLYLEKEVNAKKIGVIALSKDSKFYAVGKGVAHKVRSDS
                     IWNAIFNDGLQDVEPTLANIIDSFIAGATLFKSKETAKEKINSQTFFGKNQALYIKPY
                     LAEVTIKKDATLAKLDRDGLRYNTEKMSIQHINESCIEYIEDNGNSVMSKANTLGK"
BASE COUNT         8620 a         6091 c         4906 g         7902 t
ORIGIN      
        1 ggaataatat tcgtttagcg gtcacaaaaa gaaattgtga taacaattac cagttatgca
       61 actaacaggt taatgacaat tactatctca taattattgt gattttgtct gtgcataaca
      121 aaaaatttgt catgcacaga actgaagagg gtttccgttt aacaaacacc ttctctatga
      181 tcatgactgt caaaatgcag tttaccgtct ttcactcgca aagtgacatg accaccatgc
      241 attaatttac caaacaacag ttcatcagca agaggctttt tgatattttc ctgaatcaat
      301 ctggccatag gtcgtgctcc catggcttta tcataaccat gctccatcag ccattccctg
      361 gctgacttat caactgtaaa agtaacgcct ttatgactta attgttcatc caactccata
      421 atgaatttat caacaactag tcctatagtc tgagtatcta acggtgcaaa attgataata
      481 gcatccaaac ggttcctaaa ctcaggactg aattgctttt taattacttc caacccatca
      541 ttggtattgt cttgcgttga aaaacctatg gagttacgaa caatttcact ggcccctgcg
      601 ttactggtca ttacaagtat gatatgtctg aaatcagctt gacgaccgtt ggtatcagtt
      661 aaagtgccat gatccattat ctgcaacaat aaattaaaga catcaggatg tgctttttct
      721 atttcatcaa gtagcagaac agcatgtgga ttcttagtca cggcttcagt caataaacca
      781 ccttgatcgt atcctacata gccaggaggt gctcctatca aacgtgaaac agtgtgtttt
      841 tccatgtact cagacatatc aaaacgcagc aactctatgc ctaacacatt agccagttgc
      901 cttgttacct ctgtctttcc tacaccagtt ggtccggcaa acaagaaaca acctactggc
      961 ttctgaggtt ctctaagtcc ggatctaccc aatttgattg ctgaagccaa ggcagtaatt
     1021 gcctggtctt gaccatagac caataatttt aaatcacgtt ccaggttacg cagagtatct
     1081 ttgtcgcgcg cagatacctt tttaattggg atacgagcaa tttttgcgac aacactctcg
     1141 atttcagtca cgcttataat ttttttacgt ttatttgccg tcaataaatt ctgataagcg
     1201 cccgcttcat ccaccacatc aatcgctttg tcaggcaaaa atcgatcatt gatgtatttt
     1261 gcagataatt ctgctgccgc ttttaatgaa ggaattgaaa attttactcc atggtgagct
     1321 tccaatttgc cttttaatcc ttttaatatt tcaaaagttt catcgattgt tggttctgaa
     1381 atatcaattt tttggaatcg tcttgccaag gccctgtctt tttcaaatat tccacgatat
     1441 tcctgataag tagttgagcc aatacatttc aattcaccat ttgccaataa tggcttaatc
     1501 aggttggaag catccatgac tcctccagat gcagcacctg ctccaataat ggtgtgaatt
     1561 tcatctatga ataaaatggc tccttcttgc tgacccaact gtttcagcac agctttcaat
     1621 cgtttttcaa aatcccctct gtatttcgtt cctgccaata acgcgcccaa atcaagagag
     1681 tagactacgc agcctcttat ggaatctggg atttcaccat ccactatacg tctggccaaa
     1741 ccttctgcga tagcagtttt acctacacct gcctcgccaa ctaataaagg attattttta
     1801 cggcgtctgc aaagcacttg tatagttctt tgtatctctt catgccgccc tatcaatgga
     1861 tctatcttgc cctgttttgc acgtctgttg agattggtgc aataactatc aagtggagac
     1921 tcattaccct caggggtcat catgtcttct tccatagatg aattcatgtt ttcatgcata
     1981 tcattgtttt gatattttga cactccatga gaaatataat taatgacatc aaggcgagta
     2041 atattttcac gtcgcaaaaa atatacagcc tgactttctt gctcactaaa aattgcggct
     2101 aatacattag ctccagtgac ttccgttttg cctgctgatt gcacatgaaa tactgcacgc
     2161 tgtaaaaccc tttgaaaacc taatgttggt tgagtttccc tgtccaattc atcctcagga
     2221 attcttgggg ttgtttcatc aataaattcg atcaaatcac gacgtaatgc ctcgatgtta
     2281 gcatcacaag cttgtaatac atttcctgct gctggattat caagcaaaga caacagcaaa
     2341 tgttcaacag tcatgaactc atgacgcttt tcttttgctt ccttgaaagc tagattcaag
     2401 gtgaattcta gttctttatt taacattgtc attgctcctt accaacccac caccagttta
     2461 ctccggttcc atactagata ataatggatg ttgattcatt ctggcgtatt catttaccag
     2521 ggcgactttt gtttctgcta tgtctcttgt aaacactcca caaaccccct tcccttgaaa
     2581 atgaacctgt aacatcacct gaatcgcaac ctcctcattt aaatgaaaaa agtgcttcag
     2641 tacctctacc acaaaatcca ttggcgtata gtcatcattg agcaataaaa ccttatactt
     2701 tcttggccgt ttaatagctg tgctaatttc tgtgctaagt tctgtatcag ctatccctgt
     2761 ttgtattatt tcctctaaat tttgcttgct cataactaac aacctcttac tatttttata
     2821 gactccctac acgcatttgg ccagtaaatt tagctaattt taatgaaaaa tttagtcatt
     2881 gaaataatca atagcatagt gattagatat gattcactca aatgttgtac tatttgttat
     2941 tgaccaccag gttttactca agacattgag ccgcctctaa tcagtagagt agcatcataa
     3001 ttagcatgga taaagttcct gctcaatttt catgaactcg taagatgtat aaacctgata
     3061 gtcaaagata ctaccaataa tcttgaaagg ataatcccag tctttcatga acaacgcatt
     3121 ggcactgagt aaagctgtaa gtgctatatt gtcaatacgc gttgttcaga aagaccatgt
     3181 gataactccc cggtttacat atgcttaatc atggcatctg caaaaccaga gctgctgact
     3241 aacgttgctc cttgcatacc acgttcaaaa tcataagtta cggttttggc ttctatcgcg
     3301 ccttccatac ctctgataat caaatcagct gcttcatacc accccatatg acgtaacatc
     3361 atttcggcag aaagaataat agaaccgggg ttcactttat tctgcccagc atatttaggc
     3421 gcagttccat gagttgcttc aaacacagcc atttgatcac taatgttcgc tccgggagct
     3481 atacctattc caccaacctg agcagccaaa gcatcagaaa tataatcacc attcaaattt
     3541 aatgtagcaa tgacactata gtcttcaggt cttaacagga tttgttgcaa aaaggcatca
     3601 gcgatcacat cattaatgat aatctgctta ccggttttag gatttttaaa ttccatccat
     3661 ggcccacctt gatattcttt tgctccaaaa gaatcgcgag caacttgata tccccaatct
     3721 ttaaaagccc cttcagtaaa cttcatgata ttgcctttat gtaccaaagt cactgtactg
     3781 cggtcattat ctatcgcata ctgaatggcc gctttgacaa gtcgagtggt accttctctg
     3841 gaaacaggct tcacacctat accacaatgc tcaggaaaac gaattttctt aacacccatt
     3901 tccttagtta aaaattgaat cactttttta gcttccgggg tatctgcctg ccattcaata
     3961 ccggcataaa tatcttcaga attttccctg aaaatgacca tatcggtttt ccatggctcc
     4021 cgaaccggac ttggaacacc attaaaatag cgaattggac gtaaacaaac atagagatcc
     4081 atgtcttggc gaatagcgac attcaatgaa cgtataccgc caccaacagg agtagttaaa
     4141 ggacctttaa ttgaaaccac atattttttc atggcatcca gagtctcttt gggaagccat
     4201 tgatcgccac cataaacctt agtagccttt tccccggcat aaacctccat ccaggaaatt
     4261 tttctcttgt tgccatatgc tttttgaact gcagcatcaa caacacggat catcggaggt
     4321 gttacatcaa caccgatacc atcaccctca ataaaaggaa taattggatt atcaggaaca
     4381 tgaagtgaat ggtcagcagc aacagtaata gcttcgcctt gggcaggcac tttgatttta
     4441 tcgtatgtca ttgacgactc cataaattta gagaaattaa tgatagcatg tgataatcgg
     4501 gtttcccaaa tatcaattac gactattaat tatttttagc taaattagct cagattggaa
     4561 ctaggcatga agcaacatct taatttgttc tgccaactga tcgtaaccgg cctcgacatt
     4621 aatataatca cgcttagcga gatagactga taaagatcca tcatctgtag ccgaaggttt
     4681 ttgtaaaaca ccattaaaac cttgcatttt taaacgcaga taatcactta atttagtgac
     4741 cagataaaaa ttcctaaaat aattgtcagg attcatatac aaagcttttg catccttggc
     4801 aagttcatgt ccgggcaaat aatcttttaa ggaataggag gagttattat gaacagcgat
     4861 tactttaccc tcaggtagta agaccttaat tttgtctgca agtttcttca cttcctgatg
     4921 cgcttcagga gtataataac tgtattgcgt caaagttttt ttgatccctt tgtcggtaaa
     4981 aatgcggttg ggatcaaatt catagcgttg gttatgtaaa tggaatacta tattgcgacc
     5041 gccggaatgg ataagtgtaa tgagacttcc accctctttt ctaatgacag ctttagcagc
     5101 ttttaatgcc gtttgttcat tgtgatgaac atgtacaaaa gtcttacctt gacctttggt
     5161 ttgttgaatt attacaggtt catccccaac cataactctg tgattcactg cacaacttgt
     5221 ttgagcgata attgccataa caaccaatac ccatttcatg aataagagtc accataacga
     5281 ataaaattgt gtaattgtat agtgattggc cataaaaaat caatcttggc ttaagttcca
     5341 agcgggacaa aatcatattt taattaatta atgcacctca taccttattg cgtgaatgac
     5401 aaaatataac aattatttac gctgtttatc gtgcttaaag gcttgatctt gccctgaagt
     5461 ggttgctgtc aataaccaaa aaatttatca ttaatttcca caatcaacgc aatcataaaa
     5521 tgccgcaatt aatacttttt aataaacctt atggaatcct gactcaattt actggcgaat
     5581 tacccgaaca aactctctcg gcatttatta aacttccggg tttttatgcg gctggacgtc
     5641 tggataaaca aagtgaaggc ttacttatct taactgacga tggaaaacta caacaccgct
     5701 taacccatcc taaatttgag aaaaagaaac attactgggt tcaagtagag ggggtcccta
     5761 cagacaaaga tttacaacct ttaagaaaag gcctggtaat caaagacatt acttttttac
     5821 cagccgaagc cagattgatt tcagagccaa aaatctggcc acgaattcca cctataagag
     5881 aaaggaaaca cataccaact acctggctgg aaattatttt aagagaagga aaaaatcatc
     5941 aaataagaaa aatgaccgct gctattggat ttcctacttt acgtttaatt agataccaga
     6001 ttggtgattg gcacttagat caactccagc ccggggaata taaattaatc aaccttacta
     6061 acgataaact gaacgagtta acaaggaaga gtaacccaag actcgaatcc agggccaaat
     6121 aatcataccc aaaatggcac ttgtcacagg cattatcagg ttataattaa aacctaacag
     6181 agcatcaata aatataataa ttgattgata tagaaaacaa aaaaaaccaa tcaaacaaac
     6241 ctgctgaata agtgagaaaa attgaactcg tctcgatttg gttgacgcta tccaggtcac
     6301 caatagcaat gcgaatgaat gttcaccgat cacggttgat aagagcacat ccagtaataa
     6361 gccaactatc aacagagcag tcagtttaaa tttacctggt aaataatatt caatatataa
     6421 aaccaataat aacacccaag gaggtcgaaa aattgaaatt aactcaggca tcggcagtat
     6481 tgataaagca agtgccacga gaaaccccaa tcctaaacga atgtgaagca cgctcatgct
     6541 gtagactcct cagcattcaa tcgctcatta atttgagcag ttaactcctc ttgttcacta
     6601 tcagcccaaa tcaataacac caacctgttg cgattcagca atgcgacagg gctaactgta
     6661 actttgacaa aatcttcacc aggaatactc ttaacttcct caacgcgtcc tacaggatag
     6721 ccctctggat atcttcggcc taaaccggac gttaccagca catcacctgg atgaatagaa
     6781 gaagtcttcg gtaaattaat caaagacagt tcttcaatat cattagtacc tacaaggatt
     6841 gcgcgctctc cggttcgatt gtttcttact ggcacagcac tttttgaatc tgaaatcagt
     6901 aataaagtgc ttgtcattgg tcctacatca ataacctgcc ccatcactcc cttggcatcc
     6961 aacacgggct gtcccacata gacaccatca cgtgtgcctt tgtttaagac gacaacttgt
     7021 cttgcctggc ttgtatcaac agcgagtatc tgggcagcca tgactctcat atcagccttg
     7081 gatgaagtca ataataattc ttttaattgc gaattttctc tttgaataac aattaatttt
     7141 tgcaattcag cttcaagcat tgtttgcttg tattttaaac gcatattttc atctatcagc
     7201 gcctttttgg cactaaccaa cgactgaatc cagccgatga ctcgaacagg ataatctacc
     7261 gcatattgca agggagaaac tattagtgaa aaaccgctac gaacgaaatc caggtatcgg
     7321 taatgataat cagaaaacat caggagaata gaaaaagcaa gggcaaacac aaatcccaga
     7381 gaactgtgtc cgccctttgc aaataatcta ttgtgtttat tattctgttg agaggaaatc
     7441 accgccgcgc aaatccatgg tttctaaagc tttaccacca ccacgcgcaa cgcaggttaa
     7501 tggatcttca gctattaata cgggtaatcc agtttcttcc atcaacaagg tgtctatatt
     7561 tttcaacaga gcgcctccgc ctgttaaaac catacctctt tcagcaatat ctgcagccaa
     7621 ctcaggcgga gcaagctcca aagcagctcg aaccgcacca actatccccg atagaggttc
     7681 ctgtagtgct tccaggattt cagcgcttgt taaagtaaag ctacgtggta caccttccgc
     7741 aagatttcta cctcgtacct cgatctcaaa taaatcccgg cttgggaaag ccgaaccaat
     7801 ttcatgttta atgcgttcag cggttgtttc tccgatgaga gtaccataat tacggcgtac
     7861 gtaggatacg attgcgtcat caaatttatc gccaccaata cgtactgatt gatgataaac
     7921 tattccactt agtgaaataa ttgcgacttc tgtggtacca ccgcctatat caaccaccat
     7981 agaaccactg gcttcctcaa caggcattcc ggaacctaat gccgcagcca taggctcctc
     8041 aataaggaaa acttctcgag caccagcgcc cattgcagat tcacgaattg ctctgcgttc
     8101 cacttgtgta gaaccacaag gaacacagac caaaactcgt ggactgggtc gtaaaaattt
     8161 attttcatgc actttgtgta taaagtgctg caacattttc tcggtgacaa aaaagtcagc
     8221 aataacccca tctttcatgg gtcttatcgc attaatattg ccaggggttc ttcccaacat
     8281 gcgtttggct tcaagaccta cagcggcgac acgcttctgg cctgattcat tacgcaatgc
     8341 cactacagaa ggttcattga gaacaatccc cttatctctt acgtaaatca atgtattagc
     8401 tgttcccaaa tcgattgaca aatcgctaga aaacacgcct cttaatttcc tgaacatacg
     8461 cagtactacc ccgttgtttt tttgattgct actttatcct atatttggat aaaaatcgac
     8521 agttgccgca taagaatttt atacaccaga caactgcttt taaccggatc ccatagtaaa
     8581 agcaatgctt tcatatggga tcaatctcat ttgctttata aaacatgatt tacgttttat
     8641 attgcacgtg aattaattaa taacgaaaat ttctttcagt ttttctctat ttttatctac
     8701 aaaacgctgc cctataccct tgacttccgc aagctcttcc agagatttaa acccttgatg
     8761 attctctcta taagcaataa tagcttcagc ccgttttttt cctatccctt taaaagagcc
     8821 tgttaaagaa tacacatccg ctgtattaag attaatcttg cccttaacaa ggacttgttt
     8881 agctggagtt ttttgaggct cgttctcagc ttgagaagag aaagggataa caataaatga
     8941 caataaaata gcaatgagtc ttgctttcat aaatttctcc taattgttgg actaaacggc
     9001 tcgttccaga gccgcgagcg agtatctcaa tttttcaaac aagagtctag ccaattttaa
     9061 attatttttt ctgtgaaaaa ctataaagaa cgtacgagac acatgaggtg agttttttta
     9121 ccatgtccaa atgtaagaac tcgttcggac catgagcatt ggaatggggg cctaaaacac
     9181 cagttatcat aaattgtgct ttgggaaatt gctcgcctag catactcata aatggaatgg
     9241 tgcccccctc tcccatgtaa gcagcaggtt tatcataata agtcattgat gcttcagatg
     9301 ccgctttcgc taaccaatcg gaaagcaaag gagcattcca tcccttggac cctccatttt
     9361 gtattttaaa atcaaccttt gcattatagg gagggttttg ggtcagggct ttttccatag
     9421 caacagaagc tgcttctgga tcaaccagtg gtggaaggcg catggataat ttcaaagacg
     9481 taacagggcg cattacgttc cctgcatcag ctatcgctgg aaacccatct gcaccagtca
     9541 ccgtcaaggc agggcgccat gttctgttta atattaattg ctgtttgtct tgaataacgg
     9601 gtttggcaga atctatccat ggaaattcgc tataaacttg ttcacctaga atttccgcac
     9661 attgtttcgc ttgttttatt ctctcatcag gaatatcaca atacaactga ggtaatttta
     9721 tctctccggt gttttcgtcc tctatcctgc tgatcaattg ccgagctact ctgaaactgt
     9781 ctgccactat accactggcg ctcccagaat gaacgccctc attaattaat tcaacagtta
     9841 acttaccgac caaattaccg cgtaatgacg tagtcatcca taactgctca taattacctg
     9901 ctccggaatc aagacaaata accaatgatg gtttaccaat acgctctttc agcaactcaa
     9961 tataaaaagg caaatcgtaa ctgccacttt cctcacacgc ttcgatgatt aatatacaac
    10021 gaggatatgg caaaccttgc tgttccaagg cgcgaatagc cgtgagtgat gcataagcag
    10081 aatatccatc atctgcccct cctcttccgt ataacaatcc atttttcaat acgggtttcc
    10141 atggatgtaa atcgtcactc cagcctgaca tctcaggttg tttatccaag tgcccataaa
    10201 gcaacacagt gtcatcaatt tggcctggaa tttccataaa tagtaatgga gtcctatttt
    10261 tcaggcgaac aatttccaga gtcattcctt tgggagcatg cgacttacac caattggcaa
    10321 tgtgattaac tgcctgctcc atataaccat gttcttccca ttttgcatca aagtgaggag
    10381 atttattagg gatttttatg tagtcacata aacttggcaa tatctcttct tgccactgtt
    10441 ggcatatgta atcatacaat ccttggggtt tgaacatgtg cttgctcctt gataattgcc
    10501 acaatcttat cagtggcaga ctcaattttt aatgctttga ttttattatc tatttcatct
    10561 ttttttcgca ttacgtcctg tactgcctgt aatagaacat cagctttcaa taactcgtcc
    10621 tgaatcacaa cgcttattcc caatccctgg aagtaccttg cattctgaat ttgatctcct
    10681 ctgcttactt gtgaagagat tggaattaag atatgtggtt ttcctaatgc taatatttca
    10741 tacaaagaat tagctcctgc tcgagaaatc accacagaag aagcagcgaa cagatcagcc
    10801 aactcttcat tggcgtattc aaattggcaa tatccctcca caccaaccaa tgaagaatca
    10861 agttttccct tgccacaaag atgaatgact tgaaattctg atgtcaattg tttcaatgcg
    10921 cttcgaatac aactgttaat tgaaccagcc cctaagcttc ctcccactac aagcaggcaa
    10981 ggtttggagg aattaaatcc gcataactcc aatcctttca ttcgatttcc agttaatagc
    11041 tgttgacgaa ttggagtacc cgtcacttct attttatcct gacgcttaaa gtattttttg
    11101 ccagcatcaa aagtaagaca tattttattg acgaaaggaa aggataggcg attcgcaagt
    11161 cctgggctca tatcagactc atgagcgaca acaggaattc gatttaacca ggcgcctaca
    11221 accacaggaa aggctacaaa gccacctttt gaaaaaacca catcgggttt gattttataa
    11281 aatagcaaag aagattgaat aattcccaga acaattttga aaggatcaag caagttcttc
    11341 aaactaaaat acctgcgcaa tttaccgcta ctgaccccat gaaaaggaat gtccagcggc
    11401 tcaatcatct ccttttcaat tccggaaaca gagccgatat attctacatt ccagccttct
    11461 tttcgaaatt ccttaatcaa agcgatatta ggcgttacat gtccggcagt tcctccccca
    11521 gtaaaaacaa tacttgggct cataatgttt ccctaatcaa taagctcaaa tcaattgctt
    11581 gaattgattt ggtgatggat ccaactgaaa taaaatccac tccggtttga gcaatgtctg
    11641 caatattatt gatattaatg ccgccagagg cttccaattc acagtacttg ggatgattca
    11701 ttttgactgc ttgctcaagc atatcttgag tgaaattatc taataatatc ctgtcagggt
    11761 gagcatccaa agcctcttgc aactcctcaa gggtttccac ttcaatctca accagtaagt
    11821 gtttatcggt tttccttgcc agactgatag catgagcaac tgaaccacag gctttgatat
    11881 gattttcttt gattagaaac gcatcataaa gtcccatacg atgatttaac ccgccaccac
    11941 aatagacagc gtatttttgc gctaaccgaa gtcctggaat tgttttgcgt gtgtctaaca
    12001 gctttgcctt ggtacctttt aatttctgaa catagtgata agtttgtgtt gcagtagctg
    12061 aaagggtttg caaaaaattt aacgcagtac gctctgccgt taaaatacta ctggcagtac
    12121 catgaataat acataaagta gctggcgtgc ttaaaaaatc tccttcagaa acccgccact
    12181 ccatttcaac tgtattatca acttgcttaa aaacctcatt tacccagggc tgcccacaaa
    12241 caagcatagg ttctcttgat ataatttctg cttctacaat cagctgctga ggcaacaaag
    12301 ccgcagtaac atcaccattg cccacgtcct cttgcaaagc acgttttaca tcaaatgtaa
    12361 cttgcgataa aacattattc atgcattcct atcctctttt ggtttggcat atcgcatctt
    12421 taataaaggt ggcgcaataa aggttgtgat catgatcatc aatacaattg cggtaaacag
    12481 attatcactt attacgccta tagttctgcc aatagaagca aaaaccaatc ctacctctcc
    12541 acgaggcaac attcctatac caatgagcag cctatcatcc cgtcgattag cgcctaagcc
    12601 actaagcagc ttgccaagaa tggccgctac tgataagcct cccgctaaaa caatgacatt
    12661 ccaatcataa aaagtttcta acttaacctg tatcccaatg agagtaaaaa acataggagc
    12721 caatatagtt tccaagggag ataccaaatg atgaatactg cgatgatctt tatgatgggc
    12781 tttttgctct ccaaaatagt cgtcgtgcaa aatgacaccc gcagcaaaag caccaattat
    12841 agccgccaac tgtataaatg aagccaacca ggctaatgcc ataataaaaa ggaatgagat
    12901 aaataatttg gattcccaag gctccagaaa acggaataaa tcgactgctt tccgaagtac
    12961 aaaaggcccc ataaacaagg taccaatgaa aaacagtaga gctaaaatga taattttcat
    13021 tacaaccatg atgcttacag caccactgat aactatactg ctgactaccg ccaaaataat
    13081 caggcctaaa atatcatcca aagtagccgc accaagaatt gttcttgctt cccgtgtact
    13141 cagtttattc atctctttaa gaacacttgc tgtaatacca atgctggtag cactcaaggt
    13201 agcgccgata aataaattaa tcttgtaatt tgagttaggt atgataaaac aagccactac
    13261 aaaccctaaa atcattggcg caacgatacc gatcacagca acaagaatgg attctttgcc
    13321 agtttttttc aaatcagaaa gtgacgtctc aagaccaacc ataaaaagaa gaaatatgac
    13381 accataacgt gagaaaatat caacgacata ggctatttta agatattcag ttccctctgc
    13441 tccacttaag gcatggatca cctggttagc ataataggaa ttatggataa catgagttac
    13501 cgcctgattt aaagaaattc ctgctaacat ttctttgaca acatcataaa tggcagaacc
    13561 ttcgcgaaga attactacta attcacttcc gaaaaagtaa gagagattcc caactatcat
    13621 ccctatcagt aattcaccaa gaacgccagg ttgattaaga agtctggcta aataacgacc
    13681 aatgatagct aaaaataaaa tagtagtgac acccagaaga acagaggtga ctggatctga
    13741 atgctctccc ccctcagatg caaaagtaat agcaggaatg aaacagacta aaagaaacca
    13801 ccattttgaa ttactcatac cgctttatcc tataagctta atatgctgct ttctcccaaa
    13861 tgcaagggaa ttttgaattg caacaccata ccatcacaag tagttccatt actaacagcc
    13921 aaaataattg tattgatcaa aaaaactcat cttccaaggc aatctgctgc atttcaaaat
    13981 cataggaatg cctcagtaaa ttcctaatgc accaacagcc taacaatttc aaactgaatt
    14041 gccagcacac tctggttcat caattcatcg attgtttagc taaaaacaaa catgtttgta
    14101 aaacagagtc agggtttaga gaaacactct caattccttc cttcatcagc cattgggcaa
    14161 aatcctggtg atctgaagga ccttgaccgc aaatacctat atacttgcct tcttttttac
    14221 aagctgaaat agccatatgc aataaagctt tcacagcatc gttgcgctca tcaaattgtg
    14281 aagccaccaa acctgaatcc ctgtctaatc ctaaagtcaa ttgagttaaa tcattcgatc
    14341 ctatggaaaa accatcaaaa tactgcaaaa attcactggc taacaaggca ttggaaggta
    14401 actcgcacat cataatcact cttaacccac attttcctct ttccagacca tgctttttta
    14461 atacttcgat gacattacca gcttcagaaa ctgttcggac aaaaggaatc atgacctcaa
    14521 cattatctaa acccatctcc tccctgactc gtcttactgc tttacattca agagcaaagc
    14581 actcagaaaa atttgaagaa acataacgag aggcgccacg aaaaccaagc ataggatttt
    14641 cttcttgggg ttcatacaat gagccaccaa ccagattagc gtattcattc gatttaaaat
    14701 ctgaaagcct gactataacg ggtttgggat aaaaagcagc agcaatagtc gctatgcctt
    14761 cttttaatct ttcaatgtaa tattctacag gtgaatcata cgctatggtc ttttctttaa
    14821 tgtaccgctt aagttcctca tcctttaaag tatcaaattc cagaagcgct ttaggatgaa
    14881 tacctatcgt gttggaaatc aaaaattcaa gacgggccaa tcccactccg gaattaggta
    14941 ttgattgaaa tgcgaaggct ctttcagggt ttccaacatt caacatcact ttcatgggta
    15001 attcgggcat ggtttctaca tcaagacgct cttgctcgta aggcaacaga ccagaataga
    15061 caaaacctgt atcgccttcc gcacagctta cagtcaccac atcaccatct ttaatcgttt
    15121 ttgttgcatc accacaccca acaactgcag gaatccccaa ttcacgagca ataatagctg
    15181 catgacaagt tctgcctcca cgattggtaa cgattgcaga agcacgcttc atgacaggtt
    15241 cccaatcagg atcggtcata tcggatatca aaacatcgcc gggttgaacc ctgtgcattt
    15301 cattaatatc tttaataacc tttgctttac cctgaccgat tctttgacct atactacggc
    15361 cttcagccaa tacgtcgcct cttttttgca aagtataccg ttcgagcact tgtttgttat
    15421 ccctgctttt tacagtctcc ggacgagctt gcaggatata cagtttacca ttgaccccgt
    15481 ctttcgccca ttcaatatcc atgggacgac cataatgttt ttcaataata agtgcctgat
    15541 tcgctaattg ctctacttcc tctttggata aggaaaacaa taatctttca gcagggtcta
    15601 catcgactgt tttaacacgt ctttcaaggg aagggtcatc gcaataaacc atctttaacg
    15661 ccttgctgcc caggtttcga cggataatcg ccggttttcc ggcttcaagg catggtttat
    15721 ggacataaaa ttcatctgga tttacagcgc cttgaacaac catttcacct aaaccataag
    15781 aggaagtgat aaatactact tgatcaaaac cggattcagt atccatagta aacatgacac
    15841 cactgactgc caaatcacta cgaatcattt gctgaatgcc agcagacaat gccacttcgt
    15901 tatgagcaaa attatgatgc acacgataag ctattgccct gtcattaaat aaagaagcat
    15961 atacctgttt aatggcagcc aataccgcct cttctccctt cacatttaaa aaagtttcct
    16021 gctgaccggc aaaagaagca tcaggcaaat cttcagcagt agcagaagat cttactgcaa
    16081 cactaaaatt atcatgccca atctcttgag caagtttctg gtaggaagag cgaaccgact
    16141 gttcaaactc cggggtgaat ggcgtatcaa taatcatttc cctaatttgt ttccctacca
    16201 ctgccagttg gcagacatca tccgtatcga gagcagtcaa cttatcataa attttcttat
    16261 caagatcgtt ttgggacaaa aattctctga aagaatcagc agttgtagca aatcctcccg
    16321 gcacagaaac gcctgctgat gacaaatgac taatcatttc accaagagaa gagttcttac
    16381 caccgacctg atccaaatcg cgcatgccaa gatgtgccaa atctatagta tgtcttttaa
    16441 cagtcataac cgccccaaat tcataaaata gtaaacattc tacttaaatt gttactttat
    16501 tggaattaga acttgtgctg tatttcattt ttataaaggt caaaataccg gcattttcac
    16561 aaaaaatgac aagtatatgt actatgcggt aagatatctt gctgtccatg ttttagggtt
    16621 gaacgggtaa gcaaacctgt tccaagaata taataagcaa aaggatttga aatgtttaaa
    16681 cgcataggac taatatcgat tgccttatct ttaaatacta tctccttcac cagcgaagcc
    16741 tgttctgcct tagtgttcaa taaaaacaag cccgcaactg tcgcagtcaa tttggactgg
    16801 aaataccggg aaggagcctt tgtgattcat cctcggaatg ccatgatggt ttccagtgta
    16861 gattaccaca gcatgcatcc attaatctgg aaaagccaat atggaagtgt gatatttcac
    16921 ggaggaaatc ggtttaaagc aggaccatct gctgatggga tgaatgaaaa aggacttact
    16981 gcttcaatac tgatgcttga ttcatcaatt tatcccagta actctgagct tcccgcttta
    17041 aaaactactg aatgggttca gtatatttta gataattttc aaacggtcca agaagtcata
    17101 gatgacagct caaattatca actggttgca gacacctacc ggggaataac catgaacgtt
    17161 cacttgatgg tgagcgatgc cgaaggaaaa tctgctgttt ttgagtacct ggatggtaaa
    17221 ctggtaattc atactcaaga caacttgacc acgccagtac taaccaatac ggactataaa
    17281 ttgtcattgg ctcttcttga agaataccag gaccatggtg gctctaaggc tctgccaggt
    17341 ggatatgatt ctaaatcccg ttttgtaaga gcagcacatt atctaaaaaa acttcccagc
    17401 tttatagcta aagaagaaca tattgcttat gctttcaacg gcctcaatgt cgtcacacaa
    17461 gcacctggta cttcttctcc aacccaactg agtatggttt ttgatatccc gaccaaaaca
    17521 atttacttcc gcagcattaa cgaggctgca ttaagagtga ttccaatgga tagcattgac
    17581 tttgattatc tttatcaacc aatcgccctg aatgtttatc aacatttgtc cggggacgtc
    17641 actgataaat ttcaacccat gataggataa attggaattc attaggccct cttgcagaac
    17701 ctgattattt tgtttaatga aagaggcaag tttaggcaag aggtctattg tgaagccgca
    17761 tggatatggc acaagcgaag ccgagggggg ataattgatt aataaccact attacaattt
    17821 attgaaaaat atttaagtac accaagaact taaaaaactg tgatatcaat gcatctatta
    17881 attaccaaag gatggggtaa tgagcataaa aaatgaatat gaaacagcaa gagaccattt
    17941 caggttatgg gacaaacaac tcaaagacaa tatactgctc gaaaagtctt gtttaatgca
    18001 cacttacaaa acttattttc ccgatgagcc tggttttatt caacaattga gtcaattttc
    18061 caaagcagta gctgaagaat tagaaccaac cgttatggaa aataacctgg atgccaactt
    18121 gcctaaaata gaacaataca atgcaattgg tcagcgcgat gatcgggtaa tccaccatcc
    18181 tgcctatgtt cgatccggcg atattattta tggatccaat ctcatgcaat acttgctaaa
    18241 gccagggcaa atgaaaaaaa cgttgagcct gtttctgctc tcatcgcatg ccggggaggc
    18301 aggacacaac tgccctattg cctgttctgc aggagtaata cgtgttttaa gtcgttattc
    18361 ccaattagag aaaacctcat attatctcga taaactcact cacccttctt tcagttacaa
    18421 ttttactgga gcccaatttt taactgaaat tcaaggtggt tctgacgttg gcaccaatgc
    18481 gacccgtgcc aatctggata agcaaaatca atggcgcatt tcaggtgaaa aatggttttg
    18541 ctccaacgct aatgccgatt taattttaat gacagctcgt ttcgacgagg aaactcccgg
    18601 aaccaaagga ttgggtttgt ttttagtgcc cggtcgcctg gatgatggca gccctaatca
    18661 ctattatctt cgtcgattaa aacagaaaat tggtacccgc accatggcaa ccgcagaaat
    18721 agattttgaa ggggcactag cctaccctat gggtgatgtg gcggaaggaa tacatttggt
    18781 tatggaaaat gtgttgcatt tatcaaggat attcaattcc ttctcagtct tgggtatgtc
    18841 tcgtcgcgcc tatcagattg cttgctatta tgctaaaaac cgccgagcat ttaaccatcc
    18901 tattattgag tatcctctgg taaaagaaag tttggctaaa ataaaatcag aaaatctggc
    18961 tatgttagcc agcgtgtttc atatggctcg ttgtcaggat gaattagatg ctcttccaga
    19021 gcctcaacaa accaaatcac aacaactctt attacgtaca ctggcgaata tcaacaagta
    19081 ctttacagcc aaacgttccg tggacaatat acatcattgt ctcgatttat tggcaggaaa
    19141 tggcaccata gagaactttt cttcattacc aagactatta agagactgta tcgtttgcga
    19201 aaattgggag gggacgcatt ttacattatg gatgcaaacc ctaagagata tgcataaatt
    19261 tgcaattgat gaattattca ttgcccattt gcaacaactt ctggaacaag ttcaggatat
    19321 caaccctcat aaagcattgt ttttcggaaa gataaatgag ttagatacaa caataaaaac
    19381 aatgaaatca gccaatcatg aggagcaaag tctgcatata aaaacaatca ttgaacatat
    19441 ggcctcgttg cttgctgctt tatgtctggc tatagagata caacaaggcc agtcgccaga
    19501 aacaaaacaa gctggattaa ctttatttat tcatgaacat atggcaagtt gtttacttca
    19561 acacgacgac tatatgaaaa tactaaataa aataattgga tagttcactc tgcaaatgca
    19621 ttaaccagca ggccgggaaa aattattcgt agtgtcaaac caaaaaaata gattttaagt
    19681 ttagcaaatt gaattacata taaaaacagg catggcaatc tttgaagttt ttcgattatc
    19741 acgttaattc tatccattcg agttgaggaa gcgcttcctc aacttgaaca gataaccagc
    19801 ttagctcact gggtaactga agccagcggc tttgcaactt caaagggagg ctgactcttt
    19861 ctgtcagaat cattaattcg gcaaacatca agagcctgag aaaattcctt ataataattt
    19921 tgctctccaa tcaattgaac aattcccatt ttacggagtt tcgcatcaac gagcgggtta
    19981 gcctcactga gcaaaactct gactccacgc ttctgcaaat tcaaaataat ttcttccata
    20041 gcctgcaaac cagtgacatc tataaacgga acccaacgca ttctgatgat caaaactttc
    20101 ggatcagtat gggtattagc caacgcactc tgaaatgttt cgacagcacc aaagaaaaac
    20161 gggccctcga taacatacac catgacccct tcgggcaaat cataattctt ttcttgtgcc
    20221 aactcttgag ataattgtcc ttctgacata cgctgaattt gaatgctgtt aaccattcgg
    20281 cgcaggaaat gcaagacggc aatgatgacc cctacattga cagcaacaac caaatcaaca
    20341 aacaccgtca gacaaaaagt aaccaataag atgactacat cagcacgcgg agctactcga
    20401 attaatttaa taaagtggcg agcctcgctc atattccacg aaaccacaaa taaaatagca
    20461 gccaatgccg ccagaggtac atccactgcc agaggtgcca ggaacaacag gactaaaatc
    20521 aaagtaatgc tgtgaataat tcctgacaga ggactattgc ccccattgcg tatgtttgtc
    20581 gccgtgcgag caatcgctcc ggtggcagca aaaccaccaa acaaaggcgc cacaatatta
    20641 gctatccctt gaccaactaa ttctctattc gaattatgtc tggttccact catcccatcg
    20701 gcaaccacag cagacaataa ggactcaatt gctcccagca tcgcaatagc aaaggctggc
    20761 ccaatcaact caatcagtcg tcccattgtg atttcaggcc aggtgaaagc cggcaagcct
    20821 tgtggtattc ctccaaagag gctgcctata gtggccactc cctcaaagtg ccaaactgac
    20881 tgtatgattg taaccaggac caaagcaatc aaagggccag gaatacgctt tagtcctggt
    20941 aatttactgg aatacagtac aataaataaa gcacaaatac ctaaaaaagt agtcgcgata
    21001 ttcatatgag gaaaagacag aagtaaatgc caaaattttt catgaaaatg tccgcttcca
    21061 gctgcgggta aaccaaaaaa atattgccat tgccctaccc aaataacgac agcaatccct
    21121 gaagtaaatc cgatgattac tggactggga ataaatttaa taatggatcc cagacgggca
    21181 aagccaaaca gaaccaagat aaagcccgcc attaaagtag ctatttgtaa ccctgaaata
    21241 ccatattggg ccgttatccc ggaaagaacg acaatgaaag cacctgtagg accggcgatt
    21301 tgaactctgc tgccacccat aattgagact attaatccgg caacgatagc ggtatataat
    21361 ccttgttccg gtttagcccc ggaagctatt gcaaaagcca ttgccaaagg taaagcaaca
    21421 acaccaacaa ttaagccaga aataatattt tgtaaccaat attttttttg aaataaaccg
    21481 gctcgatacg attctgtgat cgtgttcata aataataacc tgtcccatta aaaacatggt
    21541 cattattata cgcactttga acaaatttac gctaattttc tagaatattt atcgctttaa
    21601 ttcacatctt gtcctgcatc taatattata tacgctttaa tattggaata agctacctat
    21661 catgacactt actgcattaa acgcgatttc acccatagat ggtcgttatg tcaataaaac
    21721 cagagcatta agtccctatt ttagtgagtt tgcattaacc tattatcgtt taatggttga
    21781 aatcaagtgg tttgaatcat tagctgctaa cgacactatt ccagaagtac ctgctttgga
    21841 taacaaggct cgtaaatttt tatctgatct tatttccaat tttaatgaat cagaagctga
    21901 aaaaataaaa gaatttgaaa aacaaaccaa tcatgacgtt aaagctgtag agtattatct
    21961 tcaggataaa tttcaggaaa atgaacaact caaatcttgc gttgctttca tacactttgc
    22021 ctgcacttca gaggacatca ataacctggc ttatgcttta atgatcaaac aagcgatagc
    22081 ccaagttata caacctacta tagctgagat catgggaagc attacgcttc ttggaaaaca
    22141 acatgctgac gttgcgatgc tctcaagaac gcatggtcaa ccagcaacac caaccaccat
    22201 gggtaaagaa ttggtcaatt ttgtagctcg ccttaaacga ccccagcaac aattggcaga
    22261 agtattaatc cctgccaaat ttaatggagc tgttggcaat tacaatgctc acgtagcagc
    22321 ctatcccgaa gtggactggc gtaagcattg cgctaatttt gttacatccc taggtttatc
    22381 ctttaacgcc tataccactc aaattgaacc acatgatggc atagcggaag tctcccagat
    22441 tatggtgaga attaataata ttcttctgga ttatacccaa gacatatgga gctatatttc
    22501 tcttggctac tttaagcaaa aaacaattgc cgaagaagta ggctcatcga ccatgccgca
    22561 caaggtaaat cctatagact ttgaaaatgc cgaaggcaat ttaggtctgt ctaatgcttt
    22621 atttattcat tttgccaaca aattaactca atcacgcatg caaagagacc tgtctgattc
    22681 gactgtctta cgtaacttgg gcgtagcctt ctcttacagt ttgattgcct atcactcagt
    22741 agccaaaggt aatgataaat tacaaatcaa taaatcagct ctacaaaaag atttatccga
    22801 aaactgggaa gttttagcag aggccataca aactgtcatg cgccgctaca atgaacccaa
    22861 tgcctatgaa caattaaaag agcttacacg aggacaaatg atagacgctg aaaaccttaa
    22921 aaaatttatt aaaaccctgt ctattcccga agaagcaaaa gcagagttaa tgaaattaac
    22981 tccagagact tacacaggtc ttgcaaccca attggtcaag gctttttcat gagtgatcag
    23041 ctatcacaac aaggccaatc gttcgaattc gatgttctcg tcattggaac aggattagcc
    23101 gggttacatt attgtttgca gcttttagct atccaaccca agctaaacat tgcgttaatc
    23161 agcaaagcag agtccattga gtgtaacagc cgctatgctc aaggggggat tgctgccgcc
    23221 atttcgccag aagattccct ggagtctcat atatctgata ctgtattggc tggagatggg
    23281 ctatgctact tacctgcggt ggaatttatt atccgccaag ggccagatat tataaaacaa
    23341 cttgatcagt ataaagttca atttaaaaga gaaaatgatg gtcgctttca tcttgccaaa
    23401 gaaggtggcc attcacaccg gcgcattttt aattgtggcg atcaaacggg attaacgcta
    23461 acacaaactc tcaatcattt ggcaaggcaa catcaacaga tccagttttt tgaacatcac
    23521 gtggcggtta acctgattac tcaatatcat cctcaccgga cagataacca gggtgaggta
    23581 ttaggagcct atatactcga ctgccaacat aatcgcattc acacctttct tgcgaaatgc
    23641 gtcattttag cgaccggtgg cgccggcaaa acctacaggt atacgactaa ccctatggtt
    23701 gcaacaggtg atggtgtcgc gatggcatac agggctggag ctcgtgtggg caacatggaa
    23761 ttttatcaat ttcaccctac cctcttgcac catcacacca ttaacaattt tttaatttcc
    23821 gaagcagttc gaggcgaagg cgctttgctt aaaaaccctg aaacaggcga gcgttttatg
    23881 aaacgatacg cacctgacca acttgagtta gcaaccagag acgtcgtggc aagagctatt
    23941 tttagtgaaa ttgagcaagg ccaggctgga tatgtgcatc tggatatttc tcatcaaagc
    24001 aaatctttcc tgaaaaaacg tttcccacaa atttattcca cattgttatc tattggaatt
    24061 gatatgagcc aggatttgat tcctgtcgtt ccggccgctc attaccaatg cggtggggtt
    24121 cttactgatg tggacggccg caccgatctg gcacgattat acgctatcgg tgaagtggct
    24181 tttactgggc tgcatggtgc gaacaggctg gcaagcaatt ccctacttga agccttggtc
    24241 atgggttcca atgcggcacg ctgtacactt aaagacattg ctactccgtt aaaacttatc
    24301 gatcatatcc ccagctggag ctctccagga gaagtcaatg caagaagagc cagtcaaatc
    24361 aacgcccaat ggagaggctt aagaggggaa atgacttcct atgcaggcat tgtgcgcact
    24421 gaagcaggcc ttgaagatct actacaatta atattgaagc gtaagaaaat tattgaagag
    24481 tattactgga aacattgcat taccagagat tttattgagt taaggaacat tatccttaat
    24541 gcagaattga ttgttagagc agccctttcc agaagggaat cacgtggtgg acattttcgt
    24601 gaagatttcc ccaataaaaa tttcaaagcc caggaaagta ttgcaaaact gggacaggca
    24661 gacagttcat taggatagcc tggcaaagct atcccattaa caataatgaa agaacccttc
    24721 ccacaaatcc ctaatgacta gaggtagatt taatgtttaa aaattcaact acgtaccaac
    24781 ccgaagcgac cctcatggct attcgtaatg atatggctat atgtcaaagc gattatccat
    24841 tagattggta tcaagaagat tttattcctt atgcccagga atatcaggct ttacccgata
    24901 gaaagcttac gactgtttta aattggatga aaccttatct tgctaaagcc caaaatcatt
    24961 tcggtaacaa actgttactt cttgcccatt attatatggg tggagaaatt gttcgattaa
    25021 tagagcaatt cgggggcaaa atcggtgact cctaccaatt ggctttgatg gctgcgaata
    25081 accctgaaaa atcagtcata gtggaatcag cagttcattt tatggcggaa tccataagca
    25141 ttttggctaa tgaaaatcaa cacgtctata tcaccaatcc caagtcaggc tgcaccatgg
    25201 aaatgcttgc caaagacttt atggtcgagc ctgctttcct tgatttaaat gagcgttatg
    25261 gtgctgaaaa tattttacct gtttgctaca tgaatacctc tggtcgtgtg aaagccatga
    25321 ccggtgccca gggaggtgcg gtttgtacaa gttccaatgt caaaaaaatt tttcaatggg
    25381 ctcaaaaaca aaacaagaaa atcctgttta ttcctgatca acacatggga gaaaatgtcg
    25441 cttactggtt aggaattaaa aatctcgctt attggcctgg aggaactgcc ggcgctaatt
    25501 attcactacc agcgcaagac aaaaaaacac tcaagcaatt tgataaagca gagttgattt
    25561 tattcgcaag tcaatgcgcg gttcataccc attatcaacc tgaaatgtgt gattattgga
    25621 aaagcctggg atacactacc attgtacatc cagagtgccg taatgatgtc atcaaagtgg
    25681 ctgattactc aggttctact gcattcatat gggatctggt agtccatgac aaagcaggta
    25741 caaaaagtta tgccatcgga actgaaaacc acatggtaga aaacttaaag caatattgta
    25801 acaatcttgg aattcgtgtt gtaaatcttg ctgaagcccc tcaaaaagac actgaaaaag
    25861 gagttggatg tggttgtgct accatgtctc gcaatgatcc gccacatctg gtcgctctac
    25921 tcgatttact tcgccagggc aaatcgatgc cttacaatga agtaaaagca ggtgatgtgg
    25981 tcaacgaatt cacaggaacc agaaacagac tgccagtcaa cgatcagcaa tgggttatta
    26041 ataatgccaa aaaagccctg gaaatgatga ttaatatcac agaagacagg gtttaatacg
    26101 tgttagctag tgtgttgtcc gcttccttat ggtcgcggct cggatttgca tcaacagcgc
    26161 ttatcaaatc cgagccgcga ccgtaaggaa gtgggtatat taagccgaag gatcagccac
    26221 ataaaactga cattcattca aaccacgtgt gcagacatac cctcccatag tttctatcca
    26281 gctataatca acacgagcgg gtattttggt ccgcacctgc cataaaatag aaaaatcacc
    26341 tacggagttt ctataagtac aagtcacacc ctgaccataa cctgcgacta aaatattagc
    26401 tcgcacgaag cgagtatttt cttctccctg agggctattt ggggaaaaag gattttctac
    26461 ccatggtgat ggaggcacac cccattgcaa tgacgtggtt tgaggatcag ggcatgtatg
    26521 cgcataagcg ctcaaactgg tatgggctaa agccaaaact aatagtgtag aataatatct
    26581 tttcataaag actccatcaa ctctaatcct gataattcca atttattctt ggtatgatct
    26641 cattatttaa taaagctcaa gcaattgtca aattaataat caaaatgcaa tcaaccatgt
    26701 aatttttcaa ataataaatg aactaaacca ttgttagaat ttatttcttt gctataatga
    26761 ccgcgcgtgc cggggtggcg gaattggtag acgcgccgga ttcaaaatcc ggtggtggtg
    26821 acaccgtgtg ggttcgagtc ccaccctcgg taccattgag actcgttatt ttccgagtgt
    26881 attagccttc gacattacag aattaccatt gtcttctatg tattcaatac aagattcatt
    26941 aatatgctga atactcattt tttctgtgtt gtacctaagt ccatctctat caagcttggc
    27001 taatgttgca tcttttttta tagtcacttc agcaagataa ggtttaatat aaagtgcttg
    27061 atttttacca aaaaaagttt ggctattaat tttctctttt gcagtttctt tagatttaaa
    27121 caatgttgca ccagcaataa atgagtcaat tatattcgca agagtaggtt cgacatcttg
    27181 aagaccatca ttgaaaatag cattccatat actatctgat ctgaccttgt gcgccacacc
    27241 tttcccaact gcataaaatt tactatcttt agataaggca attactccaa ttttttttgc
    27301 attaacttct ttttccaaat acaaattcac tagatcgaat agctctctag agagcgttcg
    27361 gactctcaaa agactcttta aatcaagaaa tggaaatagt tcatttagaa gtacttcttt
    27421 ttgaattaac aataaagatc cattttgatt gtaaggaata acaatatttt tattggcatt
    27481 agtacctttt aatatattag tcaatttttt aaacattta
//
LOCUS       DAXLDT010000029        27348 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXLDT010000029 DAXLDT010000000
VERSION     DAXLDT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27348
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.029"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1617
                     /locus_tag="ACF2SP_000979"
     CDS             <1..1617
                     /locus_tag="ACF2SP_000979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358780.1"
                     /translation="VSQIESLYDERVPYYKLLSMCDIYGTSYSNSIFSISLHEKKLCE
                     ELNKKLKPFMKDNSLRTDLDENQITQVEAICRNLSPDIKKQLDEKRDSILKHLENKKV
                     NCCLTKSQAIEEILDIQRSLGGDEAVGYVFTNDRRSKEGAHFEAVIITKESIIKPVEW
                     NQPMSTALYYFDRGLSGMKFYSPRGDFLADGPEQPTQQVDHTHCGVLCVSYLKKLLQD
                     KGKALFEESLMIPLYDEENNINYVYIPPPYIMQYSQTSKFIEFYQMLMGKEISASEDQ
                     PKPHNLKQLLEESKRRALGKNDKATAEINQKILDNFDEFRLRWLKGCEEACAKRDEMF
                     NNNDRSSYNLYLAYAAKRMQDEARSPKPSVQLNTKMESIDAQLKVSEQEDMLSHERRV
                     LENELEQLPSIQNLTTEDTSVPHNENEELNTLKKDLQEYVFKRMTEAGSKDFAAHHRT
                     KMIGLKLGYSAEQKITAANKLIYALDMRDKLEQRPDEMKLNETDIGALTTSRLYKNVI
                     KSHLELFNNVLANLPEISEMNVKGASGKEI"
     gene            complement(1728..6218)
                     /gene="sidE"
                     /locus_tag="ACF2SP_000980"
     CDS             complement(1728..6218)
                     /gene="sidE"
                     /locus_tag="ACF2SP_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SidE"
                     /protein_id="HFD2358781.1"
                     /translation="MPKYVEGIELTQEGMHAIFERMGHPNITSGTIYNGEPTIDKGAL
                     DRQGFMPVLTGVSPRQDSGHWIMLIKGQGNQYFLFDPLGESSGKYYQNILAKKLPGAT
                     LSVIPNDAGLNMGLCGYWVASVGLRAHAALTQPIPPSLRNLGQTITQEMRDELTQDGS
                     EKITQWLRAVGNEFPEGDIQPDATALRRATEKNLRIDELQPVFAGTTPKEISINPTAP
                     QETSVPTWNGFSLYTDEIVRNAARYAYDNYLGKPYTGTVEATPVNFGGQMVYRQHHGL
                     AHTLRTMAYAEIIVEEARKAKLRGESLKTFADGRTLADVTPEELRKIMIAQAFFVTGR
                     DDEESSKNYEKYHEQSRDAFLKYVEDNKSALIPDVFKDEKDVKFYADVIEDKDHKWAD
                     SPAHVLVNQGHMVDLVRVKQPPESYLEYYFSELQPWIGSKAAEAVFAAQRQFFHATYE
                     AVAGFNSENTEPHLVVDGLGRYVIEPDGNPIREETDDEDEEESGELKFFSQKKKLEKN
                     QRYMRVDEYLKLDEVQKRFPGAGKKLDGGLPGLSEYQYLQRLNSINRARCENDVDFCL
                     GQLQTAHHQTKITPIKRAFQSSSEKERRQPNMDEIAAARIVQQIMANPDCIHDDHVFL
                     NGQKLEENFFRDLLAKCDMAIVGSLLNDTDIRNIDTLMQHERNTEFHSTDAKAKTVKL
                     GETWEKTIRSGGGVTQIKHDLIFLMQNDAWYHTRVNAIAQNRDKDSTFKEVLITALMT
                     PLTNKSLIDTSRSPAPKTLFRGLDLSEEFKNKLINQAETIIANTTEHLFTDLSTEAFK
                     QIKLNDFSQVSARTCASTSTNIEVPRTIFGSNTIFEILDPDGLLHPKQVGTHVSGSES
                     EYSIYLPEDVALVPIKVSSDGKTVKGKDRHIFTLVAVKSPDFTPRHEIGYAVGPLLRM
                     QTPKLAETQRTVEQAREEPDLERVFNLQSRVARQAKLFSTESGYKTFLNEKVAPVLEQ
                     SLNGLLDNNVTILGKVLSAFPSDGQWSAFNSVEARQMKIQIDAIKQMVEKKAVLEGRI
                     LPALAQCQDALEKQNIDGALQALKNIPSEKEMQTMLSISSGLRGQIQRTKQNLTENLE
                     PLQRAMTAKLVSDQEKVKVRYEKLIAGIPQQITDLEKAALSDLAGVKKVVSHFNHLQE
                     ELKLLRNEKVRMHTGSEKVDFSDIAQLEAQLQKIHTKLYDAYLVELTKEISVLAKEKP
                     KNLADVKRMVSNFYAMSADIEQLRKEKIKKHGESKDPIDMSDIDKLKEELQKINQFLV
                     KAMGTNIRVSMNQMEVKTFDAQEKEAQQNLKQLDALINKLESSDAVQKQKEELEKLNQ
                     LLAEKRKAYPAMVQLQFRSEALIIHLRELCEAHQDHLAKTRKTRAQEVTNGRWKVQWL
                     TDWVGLTTDERVTLANKEKELAKFKEDLNNDEYDLQELISNLAEKNPSELEEAIGISK
                     ESAQKLHKLLSHLNHSTTFMSKIEQRLQSIDELLNEFGKQSPRTEIIKTVEEKQGTLL
                     RL"
     gene            complement(6636..7118)
                     /locus_tag="ACF2SP_000981"
     CDS             complement(6636..7118)
                     /locus_tag="ACF2SP_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HFD2358782.1"
                     /translation="MATYYGQCACGKIQFMCTGEPVFTQYCHCNKCLEIASLSQKKQD
                     KSGYSLTAAYLTRDFRIVSEENDFEEIIKEKAKLFLCSYCHSLVYGIALDPAQQDSIG
                     INVNNFSFDTSIPDSFKPVRHIWYASRIMDCDDQLPKFKDAPKEQFGSGELFELPNAN
                     "
     gene            complement(7213..9192)
                     /locus_tag="ACF2SP_000982"
     CDS             complement(7213..9192)
                     /locus_tag="ACF2SP_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358783.1"
                     /translation="MRYVNFELLKRIPQHLKGVMEYYPDVLLFQLNQIQYTHLWHELS
                     SAKYLYTNGFVIKPTHWLMYAFQTVKGWLGFDNHCQPEKISYVLDKLSYYGYTKQFLQ
                     PDFSSITNFSISPEISALVVKSRDDLTTAQLQNKLVNNYFKVEPHLGIDYSYQKLNPN
                     HRFGESWTHAHEWGLIPQLDPQDDSLIAEVISTLDKTGISAKDVFFLQHSKYAKAAAQ
                     YYCDKAKNTIVPSFFFRLLWTDPRPRYLALALAYDPEIAKRDAQKFIEYHLAQKEYDN
                     AFNLLGILNDSYLVLKFLLAIPETERHALIQKDTAIAAIMAKYYTEKKQYLLAIQFYS
                     NIEEISPNAAFAIEIQEQNYEKAYDIFKKYNSPDLFSMPERKSLAKIFYSEAETAYVA
                     GKTYRGNKSWEKAKQFYLQSLEQKRAAHHLDPSDEYLEDLYAHKRLYALLLIDADIDL
                     NKAEDSDIASIQKAITLLRECRSNNKEEQEHHKRDLAAGLMRRVDTLREKIAFNYYSS
                     DFESIRKHKIEHQQDIAILIKTLGELIALLEGTQDKALRLQLGKAHYLLADVQCFFDI
                     NAPDINQHYKMAMKAVPGNPFYVLRVAELFEEEKSKLQKIGITQLKNMGYQVFDFLHW
                     SEERWCKRDDIIHNIKDIHMPPSEPINSSSWNFTF"
     gene            9474..10268
                     /locus_tag="ACF2SP_000983"
     CDS             9474..10268
                     /locus_tag="ACF2SP_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2358784.1"
                     /translation="MATLKIHGVDIYYELYGQGKPLVLIAGYCCDHTFWNAMLHELAE
                     EFQVLIFDNRGIGQTRDNGDSFTLEAQADDIMAFLEQLGFRNPSILGQSMGGAIAQLL
                     ARKHGKKISKLVILNSVAKFNTRANQAMESLLNLRKENILFDLLIEAGIPWFFSSEYL
                     AEPKNIATFKENLKNNPYPQSLKDQARQFRTIPPFDSRGWLHEIKVPTLVIAAEDDIL
                     TLPAESQQLAQGLPNAQFITIPGGHSSPLEHPAIVNDAILKFLTSS"
     gene            10285..11751
                     /locus_tag="ACF2SP_000984"
     CDS             10285..11751
                     /locus_tag="ACF2SP_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFD2358785.1"
                     /translation="MEIYEMYIDGKFTLAKSGTTRDIFDPANGELIARVPESAKEDAI
                     LAIKAARKAFDEGEWRKSLALDRGKLLFKLADLIRANAKMLAELETRNCGKPLPEAEF
                     DVTDAANCFEFYGGLATKIHGETMSVPANSFSYVVREPIGVCGQIIPWNFPLLMAAWK
                     LAPALAAGNTAVLKPSELTPITALELFKLIDQCGFPAGVVNLITGTGIGVGEELANNA
                     MVDKVAFTGGSATGRKIMQAATGNLKKISLELGGKNPNIVFADCDLEMAIDGALFGAF
                     ANQGEVCSAGSRLLVERSIHKKIVEGMLKKIPNIKLGHGLDAGVKMGPLVSSAHREKV
                     ESYVKLGMEEGAKLLCGGKRPTGSEFAKGNFFEPTIFDEVKSTMRIAREEIFGPVLVV
                     IPFDTEEEAIRIANDTDYGLSGAVWTKSVTRAHRVTSQIRAGILWVNHYHPTYNEMPW
                     GGYKQSGAGRELGLYGIESYLEVKQVNINLDETPIGWY"
     gene            11756..13078
                     /gene="gabT"
                     /locus_tag="ACF2SP_000985"
     CDS             11756..13078
                     /gene="gabT"
                     /locus_tag="ACF2SP_000985"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFD2358786.1"
                     /translation="MKKIHIKTPIPGPKSQQLMELRRQHVARGPFHATPIFVKQAKGS
                     FVEDVDGNVFLDFSSGFGVVNTGHCPDSVVNAIKLQAEKFIHTGFNIIPYESYIKVCA
                     KLNDHTPGHFEKKSLLLNSGAEAVENAIKIARAYTGKQAVICFDHAFHGRTYMAMTLT
                     SKNKPYKHGFGPFPSEIYRAPFPYEYRWKGTNCVEECFNEFTDLANFRVGVENIAAVI
                     IEPVLGEGGFIQSPALFLQKLREFCTTNDIVFIADEIQSGFGRTGNLFAMNTLGVPPD
                     LTISAKGLGGGVVLAGVTGKAEIMDAAMEGGLGGTFGGNPLSCAAALEVFYIFEEGSL
                     LQNVTHLAKALQSRLSGFKEKYKVVGDVRGLGVMQAIELVKDKNTKEPNKEAAVQLAQ
                     FCLEHGLIILTCGTYGNVIRLHMPLSTGVKDLETGLSIIEEGLKRLSI"
     gene            13167..13925
                     /locus_tag="ACF2SP_000986"
     CDS             13167..13925
                     /locus_tag="ACF2SP_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HFD2358787.1"
                     /translation="MWYQNIMRNLLEKLPEASRKDGEVFLSKLFLAVKKVDNKPEMEF
                     FLGLIEKSFLKFENEKPGSVTLVKFSRYILGLSLLYAKSGNDLAIWNSDFIPYLTEIK
                     KFLDTDKEGKLFNKFLNKLEVATFVSLNHQLDIDFKHLISILKANCTLETIESFTEAC
                     KGLDNDCSKGFSQMMKDIEAQMINLQFTVDFDSDSSPKITDENLENTPLQKNVHVNST
                     PKGVSILHRLGIFKLFALSGVELSDQTNVLVNKK"
     gene            14189..15121
                     /gene="glsA"
                     /locus_tag="ACF2SP_000987"
     CDS             14189..15121
                     /gene="glsA"
                     /locus_tag="ACF2SP_000987"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFD2358788.1"
                     /translation="MSSKLLTIQLLEELVHAAELNQEGKTADYIPELANVNQELTAIA
                     VQPLGEKTLAYSNNPLHPVTLQSTGKMIPLIGLLEEFGADQLFEWVKVEPSGDDFASI
                     TRLEQFGPKPSNPMLNAGAIALCSRIPGVGEQQFRWLEHWVQKLFNQRLSINPLVFAS
                     EKRTGNRNRALAYLLKSRSNLGADVHETLDLYFALCSYEAMLDQMLYLPAVLANKGQD
                     PDTGEQILSIETCKITLAIMATCGLYDETGTHMVKTGMPAKSGVSGYTIAVVPGKAGI
                     VVLSPRVNAKGNSIRGEIMLEGLSKAMNWHFALP"
     gene            15245..16132
                     /locus_tag="ACF2SP_000988"
     CDS             15245..16132
                     /locus_tag="ACF2SP_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2358789.1"
                     /translation="MKIAVVEPIGIAMQEIRSELPGHTIIECDSRQWSDEQLIDFVKD
                     ADIIVLTNRRLSAAVINSALRLQLIAVAFAGIDHIDRDAVSKRNIPVKNAAGYANTAV
                     SELVFGLMISLARHIPDNNQRIREQGITNTGIELKNKVLGIVGYGAIGAEVVRLAKAF
                     QMTTIIYDRKSQVTLDDLFSQSDFVTLHVPLTNETKGMVNLRLLSQMKKSAYLINCAR
                     GPIVVSSDLKKALEKDMIAGAALDVFDVEPPLPANYSLWEVSNLIATPHIGFNTREAL
                     VAKGQLTIRNIKEFLLSRL"
     gene            complement(16269..16868)
                     /locus_tag="ACF2SP_000989"
     CDS             complement(16269..16868)
                     /locus_tag="ACF2SP_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HFD2358790.1"
                     /translation="MKIVVIGATGIIGKAIVAELNPRHEIISASYKNSDIQVDITSKQ
                     SIDAMYKKLNTIDAVVLATGKVHFAALKDMGEDDYKIGLTNKLMGQVNVVLAGLEYIN
                     DNGSFTLTSGILNRDPILYGSSAAMVNGALDGFIKSAALEMPRGIRINGVSPTVITEA
                     MENYGPYFRGYEPVSAARAALAYSKSIEGAQTGQVYCVG"
     gene            complement(17198..18592)
                     /locus_tag="ACF2SP_000990"
     CDS             complement(17198..18592)
                     /locus_tag="ACF2SP_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HFD2358791.1"
                     /translation="MILEFDTIILGGGKGGKTLAMDLAKSGQKIAMVENNQIGGTCIN
                     VACIPTKTLVQAAKVAHYCRKAKGYGLNTTLNPIDFKAIRARKDAVVNGMREANLKQF
                     LDSGMDLMLGHGHFIGPKMIEVTLSSPRDNQKTLHITADKIIINTGALPYNPPIAGLD
                     KVNYFTNDSLMNTDSVPQHLLIIGGGYIGLEFAQMFRRFGAEVTVIEASSEFLGREDK
                     DIAEQVFQTLSNEGIQFAVDTKINAIRQEQTEVIIEANRQGQSEIIRGTAVLVAVGRI
                     ANTAGLHLDKTGVELDERGFIKVNEFLETTAAGIWALGDVKGGAQFTHLSLDDYRLVK
                     HNLQNPQKKLSSQNRLIPYTVFLDPELARIGLTEAQARSQGRPIKIATIPAAAIPRAK
                     TQGETTGVLKAVIDAETDLILGVSIFCAEAGEILGVIQLAMELRVPYQKLRDMMFAHP
                     TLVEGINLWFAQII"
     gene            complement(18644..22006)
                     /locus_tag="ACF2SP_000991"
     CDS             complement(18644..22006)
                     /locus_tag="ACF2SP_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HFD2358792.1"
                     /translation="MMTNSWKDKLQKQLTQQFGSPKGKQLIDKYTETMSMSYCEQHTP
                     EEAIQDILQIEKLSKDNPLVIDFYEKTHLQYPLHIKLYRYETPIPLSDILPMLENMGL
                     RTYTERPYKIKTRDGRLFWISDFNVTYSQTDSFNITQVNNIFAEALININLGICENDG
                     FNKLVLSAGLSWQEITILRAYAKYLHQIGIRYSQSYIEKTIEMHAAIARDLIHFFYLK
                     FGLKRKSTTHKDISALEQKIQINIDAISSLDEDRIMRYFWSLMKATLRTNYFQKNHAG
                     LSKDYLSFKLKSSEIPDLPPGQPLYEIFVYSTRFEGIHLRSTKVARGGIRWSDRPEDF
                     RTEILGLMKAQKVKNAVIVPSGAKGGFVLKKPLHAASREQIQQEVISCYKSFINGLLD
                     LTDNLINDKTIPPQNVICYDDPDPYLVVAADKGTATFSDIANSIAKEHGFWLGDAFAS
                     GGSAGYDHKKMGITARGAWESVKRHFRELEVNIQQQDFTVVGIGDMSGDVFGNGMLYS
                     KHIRLLAAFDHRHIFLDPNPDPLKSYEERLRLFNLPTSSWEDYNLQLISKGGGVYKRS
                     SKSIPISPQVKKALAIEANALTPNELIRALLKAPVDLLFNGGIGTYVKATTESHADVG
                     DKTNEFCRINGAELHCKVVGEGGNLGFTQLGRVEFALKGGLINTDAIDNSAGVNCSDH
                     EVNIKILLDKEIREKKLTEKKRNQLLSRMTDEVADLVLQDNYNQALILSISAAHSSHY
                     SGLYQDYMKELEKWVNLDRSVEFLPDDKKLLERKTTGAGLTRPELAVLMAHTKIHIAN
                     ELLKSHLPDDSYFTTFLETGFPISLRKPYAKALPKHPLSREIIATQLSNKLVNNMGIT
                     FMYRMLMETGMSIADIACAYTISACIFQTDVLQNLIDSFQDKISLSTQYELLHHIRQL
                     LNLATRWFLHNNRLTGGIEKNIKNYGKSVKKLEQLIPKLMGGVTKEYLEKLISQFVSI
                     GIEKDMAQKIAITRALYTSLNIIEVTTQNEFDLSLTAETYFKVGSQFNLVWFRDQIAN
                     DSREGHWNTMARLSLRDELDNLQRRLTIAILTTNTKERTSEKLINYWLEQNHPIQKRW
                     EKLLEMLLGSENIDYTIFFIALRELSSLIQVL"
     gene            22203..22928
                     /gene="ceg9"
                     /locus_tag="ACF2SP_000992"
     CDS             22203..22928
                     /gene="ceg9"
                     /locus_tag="ACF2SP_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg9"
                     /protein_id="HFD2358793.1"
                     /translation="MYKNILFFSNFSRMRWNKYDGDRYQSLWEHNKRDIKHTFFGTIA
                     NGKDLREVLFQPVTASFSMLVAGAFAQLLVPYSIFMLCMGEAGFAGRTLINYFEITTV
                     ALAMPFIALLSTVGRCIATVKCLVEDQYNLAEMVEDKEVKPNKIYLDCAEVNYTVRDE
                     NNNQFIRKYYKFQYKMRGLNDELCQGIFTFRDLERAPDNDDFNGDLSSLTLYKYDILD
                     LIKRVHKDKFEEMEDENLIRISI"
     gene            23169..24122
                     /locus_tag="ACF2SP_000993"
     CDS             23169..24122
                     /locus_tag="ACF2SP_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358794.1"
                     /translation="MRLSYGLLGFMIFNLVACEKMALLTTPPKKQQLSNSPLATRAER
                     YFWRTLHEGRYQDIPQADYLLMAAYLENPYDSKLAAHLGFIHIWKITERERVKNNSPL
                     LPNEIILSKKYFADALQLDPENPIYQGFYGDTQLVEGQIFKDKQEEVRGYFTLQKAIS
                     NWPEFNYFTAGYPMSSLSADSDHFKKGLDWQWKTLDICAGEKISRQNPDFTPYMKRET
                     TIGAQRACWNSEIAPHNFEGFFMNMGDMLVKSGDPETGIKIYNNAKLAKNYNKWPYKD
                     MLEKRVLNVKANVQHFNQKSNDPNQSIMFNSGYGCVVCHQR"
     gene            24282..24623
                     /gene="arsC"
                     /locus_tag="ACF2SP_000994"
     CDS             24282..24623
                     /gene="arsC"
                     /locus_tag="ACF2SP_000994"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFD2358795.1"
                     /translation="MEKITIYHNPRCSKSRQALEILQNKGFEPIIIEYLKAPLDLEQL
                     KRLRTHLALEDLVRTNENVFKELGLSLANEAQVLEAMVKEPILMQRPIVTFKGKAAIG
                     RPPEKILELFD"
     gene            complement(24710..25864)
                     /locus_tag="ACF2SP_000995"
     CDS             complement(24710..25864)
                     /locus_tag="ACF2SP_000995"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HFD2358796.1"
                     /translation="MMFGFLNMTVWGYLLTGFVLTHITTTAVTIYLHRYQTHRALTLH
                     PLVSHFFRFWLWLTTGMTTAEWVAIHRKHHANTDMEGDPHSPQVFNIRKVFWQVPELY
                     REASKDKDMIKKYSYGTPNDWIERKLYSPHANKGIFLMLALNLFLFGIPGLTLWAIQM
                     MWIPLVAGVVNGIGHYWGYRNHESSDSSTNVFPWGLLIAGEELHNNHHTFASSAKFSI
                     KWWEFDLGWFYIRCLSFLKLAKVKKLPPKLIIDKNKSHIDVDSVKAILGNRFQVISDY
                     YKLVIFPTLRDMQYRIKNRDLKNARHLLKKPNEQLSHADQLRLKTLLKQHDNLQAVHN
                     YRELLQQIWLKTSLSNKDLLNLIQKWCSQAEESGLLVLRQFASQLKSYSL"
     gene            complement(25983..27227)
                     /locus_tag="ACF2SP_000996"
     CDS             complement(25983..27227)
                     /locus_tag="ACF2SP_000996"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFD2358797.1"
                     /translation="MSFKQLALIEPLNRAVSELGYTNPTSIQLKAIPLILNGHDLLGS
                     AQTGTGKTASFVLPILQKASQQTQTSRNRAKVLILTPTRELAIQAHESIIQYGKYLTL
                     RSAVIYGGVKSHNQIKQLDSGLEILVATPGRLLDLYQQGAVKFDEIDTLVLDEADRML
                     DMGFIHDIKKIIKLLPLKRQNLLFSATFTPEVRTLARNILNKAVEIDIAPRNTAVKTI
                     KQTVYSVDGNHKLALLSHLLHKNNWGQTLVFSRTKHGANKLVKQLAESQIYSVAIHGN
                     KSQAQRTKALADFKSGKVQTLIATDIAARGIDIEKLACVVNFDLPHVPEDYVHRIGRT
                     GRAGASGLAVSLVSTEEIKLLLSIEKLINQKLERIKIKDFEFLHNFSDLVSAKIQKFA
                     PPKAGYKGNISRKSRTAFNKSA"
BASE COUNT         8020 a         5106 c         5604 g         8618 t
ORIGIN      
        1 gtttctcaaa ttgaaagttt atacgatgaa agagtcccgt attataaact tttaagcatg
       61 tgtgatattt atggcacctc ctactccaat tccattttct caatttccct tcacgaaaag
      121 aagctttgtg aagaacttaa taaaaaattg aaaccattta tgaaagataa tagtttaagg
      181 acagatttgg atgaaaatca aataacgcaa gtagaggcaa tttgtagaaa tttgtctccg
      241 gatataaaaa aacaattaga tgaaaaaaga gattcaatac taaagcattt ggaaaataaa
      301 aaagttaatt gttgtttgac taagtcacag gcaatagaag agattcttga tattcagaga
      361 tctctaggtg gtgatgaagc agttggatat gtttttacca atgatagacg cagtaaagaa
      421 ggtgctcact ttgaggcggt tatcattacc aaagagtcta ttattaaacc ggtagaatgg
      481 aatcaaccta tgagtacggc attatattat tttgatcgtg gtttatccgg tatgaaattc
      541 tattcgccaa gaggcgattt cttagctgat gggccggagc aacccacaca gcaagttgat
      601 catacccatt gcggtgtgct gtgcgtttct tatcttaaaa agttattaca agataagggg
      661 aaagctttgt ttgaagaaag cttaatgatt cctttgtatg atgaagagaa taatatcaat
      721 tatgtttata ttcctcctcc ttatattatg caatactctc aaaccagcaa attcattgaa
      781 ttttatcaaa tgcttatggg gaaagagata tctgcaagtg aagatcagcc taaaccacat
      841 aatcttaaac aattattgga agagtctaag agaagagctc tggggaaaaa tgataaagct
      901 acagcagaaa taaatcaaaa gatccttgat aattttgatg agtttcgttt acggtggctt
      961 aaaggttgtg aagaagcttg cgctaagcgg gatgagatgt ttaataacaa tgacaggagt
     1021 agttataatc tttatctggc ttatgctgca aaacgcatgc aggatgaggc gcgttcacct
     1081 aaacctagtg ttcaattgaa tacaaaaatg gagagcatag atgctcagct caaggtttca
     1141 gaacaagagg atatgttatc tcatgagagg agagtgttag aaaatgaatt agagcaacta
     1201 ccttcaattc aaaacttaac tacggaagat acgtctgttc cacataatga aaatgaagaa
     1261 ttaaatacgc taaaaaagga tttacaagag tatgttttta agcgtatgac tgaagctggg
     1321 agtaaggatt ttgctgctca tcatcgtact aaaatgattg gattgaaact gggatatagc
     1381 gcagaacaaa aaatcacggc tgccaataaa ctcatttatg ccctcgatat gcgggataaa
     1441 ctagagcaaa gacctgatga aatgaaatta aatgagactg atattggggc attgactact
     1501 tctagactgt ataagaatgt tattaaaagt catttagagc tttttaataa tgtgttagcc
     1561 aatttacctg agattagtga gatgaatgta aagggcgcat cgggaaagga aatataatct
     1621 gaatttttga ttatcaattg ggaactaaaa caagaatcta tagttacaat cttatttctc
     1681 aatccccata cggtagccct aaatggacta ccgtggaaga taaagaatta caatcttaag
     1741 agagttcctt gtttttcctc aacagttttt attatttcag ttcggggcga ttgcttgcca
     1801 aactcgttta ataactcatc aatagactgt aaccgttgct cgattttact cataaatgtt
     1861 gtactgtggt ttagatgaga taacagtttg tgcaatttct gagcgctctc tttagatatc
     1921 cctatggctt cttccaattc tgacgggttt ttctcagcca ggttactgat gagttcctgc
     1981 aaatcatact cgtcattgtt taaatcttcc ttgaatttag ccaattcttt ctctttattg
     2041 gcaagagtta cccgctcatc tgtagttaaa ccaacccaat cagtcaacca ttgcactttc
     2101 catcgtccat tggtgacttc ttgtgccctt gtttttcttg ttttagccaa atggtcttga
     2161 tgagcttcac ataattcacg taaatggata attaatgctt cgctcctgaa ttgcaactgc
     2221 accattgccg gataggcctt tcgcttctcg gcaagcaatt ggtttaattt ttcgagttcc
     2281 tctttttgtt tctgtacagc atcagaactc tccaatttat tgatcagtgc gtctagttgc
     2341 ttcaaatttt gttgcgcttc cttttcctgg gcatcaaaag tttttacttc catttgattc
     2401 attgaaaccc tgatattagt tcccatcgcc ttcactaaaa attgattaat tttttgtagt
     2461 tcttccttta atttatcaat gtcagacata tcaatcggat cttttgattc accatgtttt
     2521 tttatctttt ccttacgcaa ttgttctata tcagctgaca tggcatagaa attcgaaacc
     2581 atccttttca cgtcggccag gtttttcggt ttttctttag ctagtactga aatttctttc
     2641 gttaattcaa ccaaataggc gtcatataat ttagtatgga ttttttgtag ttgcgcctct
     2701 aattgtgcga tatctgaaaa atcgactttt tcagagccag tgtgcattcg aactttttca
     2761 ttacgcagca actttaattc ttcttgcagg tgattaaaat gagagacaac ttttttaaca
     2821 ccggcaagat cagagagtgc tgctttttca agatcagtga tctgttgagg tataccagct
     2881 atcagctttt cataacgcac tttcactttt tcttgatcgc ttaccaattt ggcagtcatg
     2941 gcacgttgta gtggttccag gttctcagtc aaattttgct tcgtacgctg gatttgtcct
     3001 ctcaagccac tgctaataga cagcatagtt tgcatttctt tttcagaggg gatgttttta
     3061 agcgcttgta atgcaccatc gatgttctgc ttctccaagg catcctggca ttgagccaag
     3121 gcagggagaa ttcgaccttc gagaactgct ttcttttcaa ccatttgctt aatggcatct
     3181 atctgtattt tcatttgcct tgcttcaacg gaattaaagg cagaccattg cccgtctgaa
     3241 gggaatgctg ataatacttt gcctagaatg gttacattat tgtccaagag tccattgagg
     3301 ctttgttcca aaacaggcgc caccttttca ttcagaaacg ttttatatcc tgattcagtg
     3361 gagaataatt tagcttgtct tgctactctc gattgtaaat tgaataccct ttccaagtca
     3421 ggttcttccc ttgcctgctc aactgtcctt tgagtttctg ctaattttgg agtttgcatt
     3481 ctcaaaagag gtccaaccgc atagccgatt tcatgccttg gcgtgaaatc ggggcttttt
     3541 accgcgacta acgtaaaaat atgtcggtct ttgcctttaa ctgtttttcc gtcagagctt
     3601 actttgattg gaaccagggc aacatcttct ggcaaataaa tggaatattc actttctgac
     3661 cctgatacat gggttccaac ttgtttgggg tgtaataaac catcaggatc aagtatttca
     3721 aagatagtat tggagccaaa gattgtcctg ggaacttcaa tgttagtaga agtactggca
     3781 caagttctcg cagaaacctg gctaaagtca tttaatttga tttgtttaaa ggcttctgtc
     3841 gacagatcgg taaagagatg ttctgtcgta ttggcaatga tggtttcagc ctggttaatt
     3901 aatttatttt taaactcttc tgacagatcc aggccacgga atagcgtttt tggggctgga
     3961 gagcgcgatg tatctatcaa tgatttgtta gtcaatggtg tcattaaagc ggtaatcaac
     4021 acttctttga aggttgaatc tttatctcgg ttttgtgcaa tcgcattgac tcgagtatgg
     4081 taccaggcat cattttgcat gaggaaaatt aaatcatgtt ttatctgggt aacgccgcca
     4141 cctgatctga tggttttttc ccatgtttct ccaagtttca ctgtttttgc tttggcatcg
     4201 gttgagtgaa attctgtgtt tctctcatgt tgcattaacg tatcgatatt tctaatgtcg
     4261 gtgtcgttta atagcgaccc tacaatagcc atgtcacatt tcgccaacaa atcacgaaag
     4321 aagttttctt ccagtttttg accgttaagg aagacatgat cgtcatggat gcaatcagga
     4381 ttggccatga tttgctgaac aatacgtgca gcagcaattt catccatatt gggttgtctt
     4441 cgctctttct cagagctcga ttgaaatgct ctctttattg gagtgatttt ggtttgatga
     4501 tgagctgttt gtagttgccc caaacaaaag tcgacatcgt tttcacaccg cgctctgttt
     4561 attgagttca agcgctgtaa atattgatat tcactcagtc ctggcaatcc cccatcgagt
     4621 tttttgccag cgccagggaa tcgcttttgt acctcatcaa gttttaaata ttcatcaacg
     4681 cgcatgtaac gttggttttt ctcgagtttc tttttttgtg agaaaaactt taattcacca
     4741 gactcttcct cgtcttcgtc atcagtttct tcgcgtattg gattaccatc aggctcaatc
     4801 acatagcgcc ccaagccatc aaccaccaga tgaggttctg tgttttcgct attaaaacct
     4861 gcaactgcct cataagtggc atggaagaat tgtctctgtg ccgcaaaaac ggcttcagct
     4921 gcttttgagc ctatccaagg ttgaagttct gaaaaatagt attccagata agattcaggg
     4981 ggttgcttta ctctgactaa atcaaccata tggccctgat tgaccaggac atgagctgga
     5041 gaatctgccc atttatgatc tttatcctca atgacatcag catagaattt gacatctttt
     5101 tcatctttga atacatcggg aattaaagca gatttattgt cttcgacata ctttaaaaaa
     5161 gcatctctgc tttgttcgtg gtatttttca taatttttgg aggattcttc atcgtctctt
     5221 ccggtgacaa agaatgcctg ggcgatcatg atttttctca attcttcagg cgtgacatct
     5281 gccagagtac gaccatcagc aaatgttttt aatgattcac cccttaattt tgctttacgc
     5341 gcttcctcaa caatgatttc cgcataggcc atggtgcgta atgtgtgggc aagaccatgg
     5401 tgttgtctgt acaccatttg cccgccaaaa ttgacaggtg tcgcttctac tgtaccggta
     5461 tacggtttcc ccaaataatt atcatacgcg tatcgggctg catttctcac gatttcatca
     5521 gtatataagg aaaaaccatt ccaggtagga acggatgtct cttgaggagc agtaggattt
     5581 atcgagattt ctttgggtgt tgttcctgca aaaacaggct gaagttcatc aatgcgtaaa
     5641 ttcttttcag tagcacgtct taaggcggtg gcatcaggtt gtatatcacc ctctggaaat
     5701 tcattgccaa cggcccgtaa ccattgtgta attttttcgc ttccatcttg tgttagttca
     5761 tcccgcattt cctgggtaat ggtttgaccc agatttctta atgagggggg gataggttgt
     5821 gttaatgccg catgagctct taaaccaact gatgcaaccc aatagccgca taatcccata
     5881 tttaatccag catcgttagg aataacagaa agcgtagcgc caggtagttt tttagctaaa
     5941 atattttggt aatattttcc cgagctttct cccagtgggt caaagaggaa atattgattg
     6001 ccttgacctt taattaacat aatccaatgt cccgagtctt gccttgggct gaccccggtc
     6061 aatactggca taaagccttg cctgtcgaga gcacctttat caattgtagg ctctccgttg
     6121 tagatagtac cgctggtaat atttggatgt cccatgcgtt caaaaatagc atgcatccct
     6181 tcttgggtta attctatccc ttcaacgtac ttaggcatta cacaactcct ggataaatac
     6241 tttaaaatta atattcgtga cttaaaaatt agcacagtgg gtaaatatta tagctgtcaa
     6301 actttaagag aatattaaga gataaaaaaa catccggctt aagatctgat cccaatatcc
     6361 tttggttttt atcatggata taaaagacaa aaattatttg acagtactta tgaaaccccc
     6421 aatctctatg ttaaaaaatg tttttaattt ggtcatttag tttatctaaa ctccctcatt
     6481 aaaaacattt tttgccttga ccttggagtt tttcgtaaat cctgtttgaa ttagctttgg
     6541 caaaacggtg tgttgtcgga ttgaaaaaat atgagagcca gatcaatgaa gtatgctgga
     6601 tattttggaa agataaaggc ctagaatacg gtttatcagt tggcattagg caattcaaat
     6661 aattcccctg aaccaaactg ctctttggga gcgtctttaa atttaggcaa ttgatcatcg
     6721 caatccataa tacgactggc ataccatatg tgccttactg gtttaaatga atctgggatg
     6781 ctcgtatcaa agctaaagtt atttacatta atcccaatac tatcctgttg cgcgggatca
     6841 agtgcaatac cataaacaag gctgtgacaa tatgagcaaa gaaataactt tgccttttct
     6901 ttaatgattt cttcaaaatc attctcctca gacacgatcc tgaagtctct ggtcaaataa
     6961 gctgcagtga gcgagtaacc tgatttatcc tgtttttttt gagataaaga agcaatttcc
     7021 agacatttat tgcaatgaca atattgagta aagacaggct cacctgtgca cataaattga
     7081 atttttccac aagcgcattg accataataa gttgccatta ttctaaatgc ctccttcaat
     7141 tggcagtaat ggaaaagcat attttaaaga aagcccacaa accgaggtta ttttatactt
     7201 tatagctatt aattaaaacg taaaattcca tgatgagcta ttaattggtt ctgagggcgg
     7261 catgtggata tctttgatat tatgaatgat atcgtctcgt ttacaccaac gctcttcaga
     7321 ccaatgaaga aaatcgaaga cctggtaccc catatttttc agctgagtta tgccgatttt
     7381 ttgcagtttg cttttttctt cttcaaatag ctctgctacg cgtagcacat aaaaggggtt
     7441 tcctggtacg gctttcatcg ccattttata atgctggtta atgtctgggg cattgatatc
     7501 aaaaaagcat tgaacatcgg ccaggagata atgtgcttta cctagttgca agcgcaaggc
     7561 tttgtcttga gttccttcca atagagcaat taactcccct aatgttttaa ttaaaatagc
     7621 aatatcttgt tggtgttcga ttttatgttt tctgatagac tcaaaatcag aagagtaata
     7681 attaaaagca attttttcac gcaaagtatc tactcggcgc ataagcccgg ctgccaggtc
     7741 gcgcttatga tgttcttgtt cttctttatt gtttgagcgg cattctctca acagagtgat
     7801 tgctttttgg atgctggcaa tatcactgtc ctctgctttg ttcaggtcga tatccgcatc
     7861 gattaataac aaggcatata agcgtttgtg agcgtataaa tcttccaggt attcgtcaga
     7921 gggatccagg tggtgcgctg cccttttttg ctccaggctt tgcagataaa attgttttgc
     7981 tttttcccaa cttttatttc ctctatatgt tttcccggct acataggctg tttcagcttc
     8041 actataaaat atcttggcaa gcgatttacg ctcaggcatt gaaaatagat caggggaatt
     8101 gtatttctta aagatatcat aagccttttc atagttttgc tcttgaattt caatagcaaa
     8161 tgctgcatta gggctaattt cttcaatatt ggagtaaaat tgtattgcca gcagatattg
     8221 ctttttctca gtatagtatt tcgccatgat ggcggcgatt gctgtgtctt tttgaattaa
     8281 agcatgacgt tctgtttctg gaatcgctaa caaaaatttt aaaaccagat aggagtcgtt
     8341 caatatgccc aatagattaa acgcattgtc atattccttt tgcgcaagat gatattcaat
     8401 gaatttctgg gcatcccgct tggctatttc aggatcgtaa gcaagggcca aagcgagata
     8461 gcgagggcgc gggtcggtcc aaagcaagcg gaaaaaaaat gaagggacaa ttgtattttt
     8521 agccttatca caatagtact gagcagcagc tttggcgtat ttgctatgtt gcaaaaagaa
     8581 cacatctttt gctgaaatcc ccgtcttatc cagggtgctg attacctctg cgatcaagct
     8641 gtcatcttga ggatctaatt gcggaatcag tccccactcg tgtgcgtgcg tccagctttc
     8701 accaaagcga tgattggggt ttagtttctg gtaggaataa tcaatcccaa gatggggctc
     8761 cactttaaaa taattattca ccaatttatt ttgtagctgg gctgtcgtta aatcatctct
     8821 gcttttgaca accagggcag aaatttcagg ggagattgag aaatttgtta ttgatgaaaa
     8881 atcaggctga agaaattgtt tcgtgtatcc ataatacgag agtttgtcta aaacgtatga
     8941 aattttctct ggttggcaat gattatcaaa gccaagccag cctttaaccg tttgaaaggc
     9001 atacatcaac caatgggtag gttttatcac aaatccgtta gtatacaggt attttgcgga
     9061 agataactca tgccataaat gggtatactg tatttgattc aattgaaaca gaagcacatc
     9121 cgggtagtat tccataaccc ctttaagatg ctgagggatt ctctttaata attcaaaatt
     9181 aacataacgc atttattttc atctaaaata gataggaagg cgatgaagcg atgagtatat
     9241 taaaccttaa ttaagaaatt attaagagga attgatttct gcatcaatta tcttgatgat
     9301 aattgagcag tcagatttta taaaaactaa caattatgac agcaggaact tattaaacac
     9361 gtttgttgac aaatgactta attcatcgaa acaatagata attgaggcga aaagtcattg
     9421 aatgaagcgt tgagtttgtt cgtcaaaaaa atcaattaag gagtttttgc gacatggcca
     9481 cactcaagat tcatggtgtt gatatttatt atgagctcta tgggcaaggt aaaccactgg
     9541 ttttaattgc aggctattgc tgtgatcaca ccttttggaa cgccatgctc catgagttgg
     9601 ctgaagaatt ccaggtattg atatttgaca atcgcgggat agggcaaacg agagacaatg
     9661 gagattcttt tacattagaa gctcaggcgg atgacataat ggctttcttg gagcaattag
     9721 gctttagaaa cccttctatt ctggggcaat caatgggtgg ggctattgct caactgcttg
     9781 ccagaaagca tggtaaaaaa atcagtaaat tggttatttt aaattcggtt gcaaaattca
     9841 atacgagagc aaaccaggct atggaaagcc ttttgaattt gcgaaaggag aatattttat
     9901 tcgatttatt aatagaggct ggaataccat ggttcttttc aagcgaatat ttggctgagc
     9961 ctaaaaatat cgctactttt aaagagaatt tgaaaaataa tccttacccg caatccttaa
    10021 aagatcaagc gcgacaattt agaaccatac caccatttga ttcccgcggg tggctacatg
    10081 aaattaaagt acccacacta gttatagcag cagaggacga tatactgacc ttgcctgcag
    10141 aaagtcaaca actggcacaa gggctcccta atgctcaatt cattacaatt cctggtggac
    10201 attccagtcc cttggaacac cccgcgatag tcaatgatgc catactaaaa tttttaacct
    10261 cttcctaaaa aaggtgctga ataaatggaa atttacgaaa tgtacattga tggaaaattt
    10321 actttagcca aaagcggaac tactcgcgac atttttgatc cggctaacgg ggaattgatt
    10381 gcaagagtgc cagaaagtgc caaagaagat gccatacttg ctatcaaagc agcgagaaaa
    10441 gccttcgatg aaggagagtg gagaaaatca ctggcgcttg atcgaggcaa attgttattc
    10501 aagcttgctg atttgattcg agccaatgct aaaatgttgg cagagctgga aactcgaaat
    10561 tgcggaaagc cgttacccga agcggagttt gatgtcactg atgctgccaa ttgctttgaa
    10621 ttttacggtg ggcttgcgac taaaattcat ggtgaaacga tgtcggttcc agcgaattca
    10681 tttagttacg tagtacgtga acccataggc gtgtgtgggc aaatcattcc atggaatttt
    10741 ccactgctta tggcggcctg gaaacttgct cccgcacttg ccgcaggcaa taccgcggta
    10801 ttaaaaccat cagaattgac cccgataacg gcacttgagt tattcaagtt gattgatcaa
    10861 tgcggatttc cagcgggcgt ggttaacctc attactggga cgggtatagg agttggagag
    10921 gagttggcaa ataatgctat ggtggataag gttgctttca ccggaggatc ggcaacaggc
    10981 agaaaaatca tgcaggcagc tactggcaat ttgaaaaaaa tctcgttaga gcttggcgga
    11041 aaaaatccaa atattgtctt tgcagactgt gaccttgaaa tggcaatcga cggcgccctt
    11101 tttggtgctt ttgcaaatca gggggaagtc tgttctgcgg gttcccgctt gctagtggag
    11161 cgctctattc acaaaaaaat agtagaggga atgttgaaaa aaattccaaa tatcaagctt
    11221 ggccatggtt tggacgccgg ggtaaaaatg gggcctttgg tttcaagtgc acatcgtgaa
    11281 aaagtggaaa gctatgtcaa gcttggaatg gaagaaggag ccaaattact ttgcggcggt
    11341 aaacgcccga caggcagtga atttgcaaag ggaaattttt tcgagccaac tatttttgat
    11401 gaagtgaaat ccacgatgcg cattgctcgt gaagaaattt ttggtccagt acttgtggtc
    11461 atcccctttg atacagaaga agaagccatt cgcattgcga acgatactga ttatggctta
    11521 tccggggctg tatggacgaa gagcgttact cgggcccatc gagtcacatc tcaaatccgt
    11581 gccggtattc tttgggtgaa tcattaccat ccaacctata atgaaatgcc ttggggcgga
    11641 tacaaacaaa gtggagcggg tcgtgagtta ggcttgtatg gcattgagag ttaccttgag
    11701 gtcaagcaag tgaatattaa cctggatgaa acaccaattg gttggtacta aactaatgaa
    11761 aaaaattcat atcaaaacgc caatacccgg acccaaatcg caacagttga tggaattgcg
    11821 acgtcagcat gttgcaagag ggccatttca tgctactcct atctttgtga agcaggccaa
    11881 aggttctttt gtggaggatg ttgatggaaa tgtatttttg gacttttcat cagggtttgg
    11941 ggtggtcaat acagggcact gtcctgattc agtggtcaac gccatcaagc tccaggccga
    12001 aaaatttata cacaccggct tcaatattat tccctatgaa tcttatatca aggtgtgtgc
    12061 aaaattaaat gatcacaccc ctggacattt cgaaaaaaaa tcgctccttt tgaattccgg
    12121 tgcggaagca gttgaaaatg ccattaaaat tgctcgtgcc tatacgggca aacaagcagt
    12181 gatttgtttt gatcatgctt ttcatggacg cacgtacatg gcaatgacac tcacttcgaa
    12241 aaacaaaccc tataagcatg gattcggccc atttccttct gaaatctatc gtgctccttt
    12301 tccttatgag tatcgttgga agggaaccaa ttgtgtagaa gaatgtttca atgagtttac
    12361 cgatcttgcc aatttccgtg tcggtgttga aaacattgct gcagtcatta tagagccggt
    12421 attgggtgaa ggtggattta ttcaatcccc tgccttattt ttgcaaaaac ttcgtgagtt
    12481 ttgtacgaca aacgatatcg tttttattgc cgatgaaatc caatccggtt ttggccgcac
    12541 aggaaatctg tttgcgatga atacgttggg ggtacccccc gatctcacca tatcagcaaa
    12601 aggccttggc ggcggggtag tgcttgctgg tgtaacaggt aaagctgaaa tcatggatgc
    12661 ggctatggaa ggtgggttgg gtggaacttt tggtggaaat cctcttagtt gtgctgctgc
    12721 tcttgaagtg ttctatattt ttgaagaagg aagcctgctg caaaatgtta cccatcttgc
    12781 aaaggcactg cagtccagac tttctggatt taaggaaaaa tacaaggtag ttggtgatgt
    12841 tcgtggtttg ggcgtcatgc aggcaatcga attggtaaag gataaaaaca cgaaagaacc
    12901 taataaagaa gccgcagtac aattggctca attttgttta gaacatgggt tgatcatttt
    12961 gacctgcgga acatatggga acgtcattcg tctccacatg ccactttcta ctggcgtaaa
    13021 agatttggaa accggccttt cgattattga ggaagggctt aagcgactat caatatagat
    13081 aaacagtctg aaatgagata gctctcttaa tgttcactta atcaattttt gatatcattt
    13141 gttccaatta attacaggtg agtaatatgt ggtatcaaaa tataatgcgc aatttgcttg
    13201 aaaaacttcc ggaagcaagt agaaaagatg gagaggtttt tttaagtaaa ctgtttctgg
    13261 ctgttaaaaa agttgataat aagcctgaaa tggaattctt tttaggcctt attgaaaaat
    13321 cttttttaaa atttgagaat gaaaagcccg gctcagtcac cttagtcaaa ttctccaggt
    13381 atattttagg actaagcttg ctctatgcta agtcaggcaa tgatttagct atatggaact
    13441 cagattttat tccatacctt acagaaatta agaagttcct cgatacagat aaagagggta
    13501 aattgtttaa taagtttctc aataaacttg aagtggcgac gtttgtcagt ttaaatcatc
    13561 aacttgacat tgatttcaaa cacttaatct ctattctaaa agcaaattgc actttggaaa
    13621 caatcgaatc ttttactgag gcttgcaaag gattagataa tgattgttcg aaaggattta
    13681 gccaaatgat gaaagatata gaagcgcaaa tgatcaattt gcaatttact gttgatttcg
    13741 attccgactc tagtcctaag attactgatg aaaatctgga aaatactcca ttgcaaaaga
    13801 atgttcacgt caattccacc cccaagggtg taagcatatt gcatcgactt ggtatattta
    13861 aattatttgc attgtctggt gtggagttga gtgaccaaac caatgtgctt gtaaataaaa
    13921 aataatatat caatgtagtt gttctcatta ttttgttcct gaattctttc agggacaaaa
    13981 ttgatgcagg tctagagatt atcatctaat ataaaaattg cagattgagt actggggtat
    14041 gacctccaat tgatgtaaga ataataaatt cccaaagagg tcttatttcc aacttggtcc
    14101 atttatcaat aatatgcttt agaaataaaa tatttgtctt gatgtgctaa atttaccata
    14161 atggcccctt ttttaatcca atagtactat gtcatcaaaa ttgcttacca tccaattgtt
    14221 agaagagctt gtacatgctg ccgaattaaa tcaagaaggc aaaactgcgg attatatccc
    14281 tgaactggcg aatgttaatc aggaattgac agcaattgcg gtgcaacctt tgggtgagaa
    14341 aaccttggct tatagtaata atccacttca tcccgtgact ttgcaaagca ctgggaagat
    14401 gatccctttg attggtttgc ttgaggagtt tggtgctgat caattgtttg aatgggttaa
    14461 ggtggagcct tcgggcgatg attttgcttc cattacccgc ttggagcagt ttggacctaa
    14521 accgtcaaat cccatgctga acgctggcgc aattgcctta tgttcacgta ttccaggcgt
    14581 gggtgaacag cagtttagat ggttagaaca ttgggtacaa aaattattta atcaacgtct
    14641 cagtattaat cctttggttt ttgcctctga aaaacgaacc ggtaatcgta accgcgctct
    14701 tgcctattta ctgaaaagca gaagcaatct tggagctgat gttcatgaaa ccctcgattt
    14761 gtattttgcg ctatgttcct atgaagccat gttagatcag atgctgtact tacctgctgt
    14821 gttagcgaac aaggggcaag atccggacac gggtgaacaa atcctttcta tcgaaacctg
    14881 taaaattact ttggctatca tggcaacctg tggattatat gatgaaacag gtactcacat
    14941 ggttaaaaca gggatgccag ctaaaagtgg agtgtctggt tataccatcg cggttgttcc
    15001 cggtaaagca ggtattgtgg tgttgagtcc aagagtgaat gcgaaaggaa atagcatacg
    15061 aggtgaaatc atgttagaag gtttatccaa agcaatgaat tggcatttcg cattacctta
    15121 atttttaaaa aagagcgtta tagaatggtg agctatctct ttgattgaag tgaaataacg
    15181 atgacaaatt gcttgtcaca gcggagtgga aaaacttaat taaatttgga ttaaaggatt
    15241 ttacatgaaa attgccgttg ttgagcctat agggattgct atgcaggaaa ttcgttctga
    15301 attaccaggc catacgatta tcgaatgtga cagtcgtcag tggagtgatg agcaattaat
    15361 agattttgtg aaggatgcag acatcattgt tctgacgaat cgacgtttat cggctgcagt
    15421 cattaattca gcgctgcgat tacaattgat tgctgttgca tttgctggaa tcgatcatat
    15481 tgatagggat gcggtgagta aaagaaatat tccggtaaaa aatgcagcgg gttatgccaa
    15541 cactgcggtg tctgaactgg tatttggtct catgatttct cttgcacgtc atattcctga
    15601 taacaatcag agaataagag agcaaggcat aaccaatacc ggtattgaac taaaaaataa
    15661 agtgcttggc attgtcggtt atggagcaat cggcgcagaa gttgtgcggc ttgctaaggc
    15721 atttcaaatg acaactataa tttatgacag gaaatctcaa gtaacattag acgatttgtt
    15781 ttcccaatct gatttcgtca ctttacatgt tccacttaca aatgaaacca aaggcatggt
    15841 taacttaaga cttttatctc aaatgaaaaa atcagcgtat ttgattaatt gtgccagagg
    15901 gccgattgtg gttagcagtg atttaaaaaa agccttggaa aaggacatga tagcaggtgc
    15961 tgcattggat gtatttgatg ttgaaccacc attacctgca aattatagcc tttgggaggt
    16021 atctaatctt atcgcaacac cacacatagg atttaataca agggaggccc ttgtggccaa
    16081 ggggcaattg actatcagaa atattaaaga gtttcttttg tctcgtttat aaaaccgtgt
    16141 aaaaacataa agaaacataa gaagttgatt gcaaaatagc ttgtagtact tctgatagtt
    16201 tatcttaacg ccctcgttaa aaacattttt tgcctcgacc ttggggtttt tcatgagtcc
    16261 tgttaatgtt accctacaca atacacttga cctgtttgag cgccctcaat actcttgctg
    16321 taggcgagtg ctgcacgagc agcggataca ggttcatagc ctctgaaata ggggccatag
    16381 ttttccatgg cttcggtaat tactgttggg cttacaccat tgattcggat acctcttggc
    16441 atttccagag cagcgctttt gataaaacca tccaatgcgc cattaaccat ggcagcagaa
    16501 cttccataga gaatgggatc acgatttaat attccgcttg ttaatgtaaa tgagccatta
    16561 tcattgatgt attctaatcc agccaagact acattaactt gacccattag tttattggtc
    16621 aaaccaattt tataatcgtc ttctcccatg tcttttaggg cagcaaagtg cactttaccg
    16681 gtagctaaca caaccgcatc aatagtattt aattttttgt acattgcatc gattgattgt
    16741 ttactggtta tatccacttg aatatcactg tttttataac tcgctgaaat aatttcatgc
    16801 ctcgggttta attcagcaac tattgctttt ccaattatcc ccgtagcacc aatcaccact
    16861 attttcataa ttcattccct ctttatccaa taaaaagcaa aagtcctacg tagaatagta
    16921 cagtaacaac aatgagttaa atacaggact tacaaaaaac ccaatctctt tgttaaaaaa
    16981 tatttttaat ttggtcattt agtttatcta aactccctta ttaaaaacat tttttgcctt
    17041 gaccttggag tttttcgtaa gtcctgaaat aaaaagtgtg tatcggctgt atttaattac
    17101 agcctataca caggatttat tgaagaagtg aactaaaatg cctctattaa ccacttgcct
    17161 tgtagcatgt aatatggcga gtatggttta aatcaattca aattatttgc gcaaaccaca
    17221 gattgatgcc ctccactaga gtagggtgag cgaacatcat atcgcgtaat ttttgataag
    17281 gtacgcgcaa ctccattgct aattggatga ctcctaatat ttcccctgcc tccgcacaga
    17341 aaatagaaac gcccaaaatc aaatcggttt cagcgtcaat gacggctttt aaaacacccg
    17401 ttgtttcccc ttgggttttg gcgcgaggaa ttgcagctgc cggaattgta gctattttaa
    17461 ttgggcggcc ttgggatctg gcttgagctt cagtaagtcc aatgcgtgct aattccgggt
    17521 cgagaaacac tgtgtaagga ataagcctgt tttgggaaga aagctttttc tgggggtttt
    17581 gtaaattatg tttcaccaga cgatagtcat caagagacag gtgggtaaac tgtgcccctc
    17641 ccttgacgtc gcccaatgcc caaatccctg ccgctgtcgt ttcgagaaac tcattgactt
    17701 tgatgaatcc acgctcatca agctcaacgc cagttttatc caaatgcaat cctgctgtat
    17761 tggcaatacg ccctactgca accaggacag ctgtgccacg aataatttct gattggcctt
    17821 gtcgattggc ttcgataatc acttcagttt gctcttggcg aatggcatta atctttgtat
    17881 cgacagcaaa ttgaatgcct tcgttggata aggtttgaaa aacctgttca gcaatgtcct
    17941 tgtcttcccg gccaagaaat tcgctgctgg cttcgatgac ggtgacttca gcgccaaacc
    18001 ggcgaaacat ttgtgcaaat tccaagccaa tataaccgcc gccgatgatc aataaatgtt
    18061 gaggtaccga atcggtgttc attaaactat cattggtaaa ataattgact ttatcgagcc
    18121 ccgcaatggg gggattgtaa ggcagggcgc ctgtattaat aataatttta tcagctgtga
    18181 tgtgcagtgt tttttgattg tcacgtggtg aggatagggt aacttcaatc atttttggcc
    18241 caataaaatg accatgccct aacatgagat ccatgcccga atctaaaaat tgttttaaat
    18301 tcgcttcacg catcccattc accacagcgt ctttacgtgc tcgaattgct ttaaaatcaa
    18361 ttgggttcag tgtggtattt aaaccatagc cttttgcttt acggcagtaa tgcgccactt
    18421 tagctgcctg tacaagtgtt ttcgtaggta tacatgccac attgatacag gtcccaccaa
    18481 tctgattgtt ttcaaccatc gcaattttct ggccgctttt agccaaatcc attgcaagag
    18541 tcttcccacc tttgccacca cctaatatga tggtatcaaa ctctagaatc atttcattct
    18601 cctgataatt tcaatgtttt atccacttga tgatgaccct tatttacaat acttgaatta
    18661 atgaggaaag ctcacgcaag gcaataaaaa atatggtgta atctatgttt tcgctaccca
    18721 ataacatttc taataacttt tcccagcgtt tttgaatagg gtgattttgt tccagccaat
    18781 agtttatcaa tttttcagaa gtacgttctt tggtgttagt tgttaaaatg gcaatagtca
    18841 aacgtctttg aagattatct aactcatcac gcaaagacag gcgagccatg gtattccaat
    18901 gcccttcacg tgagtcattg gctatttggt cgcgaaacca aacgagatta aattgactgc
    18961 caactttaaa gtaagtttct gcagtaagcg ataaatcaaa ttcattttgc gtggttactt
    19021 caatgatatt taatgaagta taaagagccc gagttatagc aattttttga gccatatctt
    19081 tttcaatgcc aatactgacg aattggctaa tgagtttctc aaggtattct ttagtgacac
    19141 cacccatgag cttgggaatc aactgttcaa gttttttgac tgacttgcca taatttttaa
    19201 tatttttttc aatgcctccg gtcagtcggt tattatgcaa aaaccagcgt gtcgccaaat
    19261 tgagcaattg gcggatatga tgcaataatt catattgggt ggataaggat attttatctt
    19321 gaaatgaatc aatcagattt tgtaagacat ccgtttggaa aatacaggct gaaatagtgt
    19381 aagcgcaggc aatatcagca atcgacatac cggtttccat cagcatgcgg tacatgaagg
    19441 taattcccat gttgttaacc agcttattgc tcagttgcgt cgctatgatt tcacgagaca
    19501 aaggatgttt tggcaaggct ttcgcatagg gctttcgtag cgagatggga aaacctgttt
    19561 ccaaaaaagt tgtaaagtac gagtcatcgg gtaaatgcga ttttaataat tcgttagcaa
    19621 tatgaatttt ggtgtgtgcc attaaaacag caagctctgg tcttgtcagt cctgcgccag
    19681 tggttttgcg ttcgaggagt tttttatcgt caggaaggaa ctcaacgctt ctgtcaaggt
    19741 taacccactt ttccaattct ttcatgtaat cttggtataa gccactataa tgagaagaat
    19801 gagctgccga tatacttaaa atcaaggctt gattgtagtt gtcttgcaaa acaaggtcag
    19861 cgacttcgtc tgtcattctg cttagcaatt gattgcgttt tttttctgtg agtttctttt
    19921 ctctaatctc tttatctaaa agaattttga tattgacttc atggtcagag cagttaacgc
    19981 cggcggaatt atcaatcgca tcggtattaa ttagaccgcc ctttaatgca aactcgacgc
    20041 gtccaagttg agtaaaaccc agatttccgc cttctcccac taccttgcag tgtaattctg
    20101 cgccattaat acggcagaat tcattagtct tgtcgccaac atcggcatgt gactccgtgg
    20161 ttgctttgac ataggttcca ataccgccat taaatagtaa atccactggt gcttttaaca
    20221 gcgctcgaat aagctcgttg ggcgtaaggg catttgcttc tatagcgaga gcttttttta
    20281 cttgcgggct aatggggatc gatttgcttg agcgtttgta aacgcctccg cctttagaaa
    20341 taagttgtaa gttgtaatct tcccaggatg aagtaggtaa attaaacagg cgaagtcttt
    20401 cttcatagga ttttaaaggg tcgggattcg ggtcgagaaa aatatgacga tgatcaaaag
    20461 cagctaacag tcgtatatgc tttgaataaa gcataccatt accaaataca tcaccactca
    20521 tatcaccaat acctactacc gtaaaatctt gttgctgaat attgacctca agttcacgaa
    20581 aatgtctttt gacagattcc cacgcaccac gtgctgtaat acccattttt ttatgatcgt
    20641 acccggcaga acctccagaa gcaaaagcat cacctagcca aaatccatgt tccttggcaa
    20701 tactattggc aatatcggaa aaagtagctg ttcccttgtc agcagcaaca actagataag
    20761 ggtcagggtc gtcgtaacat ataacattct gtggagggat tgttttatca ttgattaaat
    20821 tatcggtgag atccagtaaa ccattgatga acgatttata acaagagatg acttcttgtt
    20881 ggatttgttc gcggctggct gcatgaagcg gctttttcaa gacaaaacct cccttggcgc
    20941 cagagggaac aatgactgcg ttttttacct tctgcgcttt cataagtcct aaaatctcgg
    21001 ttcgaaaatc ttctggtcga tcggaccagc gaataccgcc tcgggctact ttggtactac
    21061 gtaaatggat tccttcaaaa cgtgttgagt acacaaaaat ttcataaagc ggttgacctg
    21121 gaggtaagtc aggaatttcc gaggatttta atttgaagga aaggtaatct ttagataatc
    21181 ccgcgtgatt tttttgaaag taattagtac gaagagttgc tttcatcaaa gaccaaaaat
    21241 aacgcatgat gcgatcctca tccaggctgc taatcgcatc aatgtttatt tgaattttct
    21301 gttcgagtgc cgatatgtcc ttgtgagtag tagatttcct tttaaggcca aatttcaaat
    21361 aaaaaaagtg aatcagatca cgggcaatgg ccgcatgcat ttcgatggtt ttttcaatgt
    21421 aagattgact gtaacgaatg ccaatctgat ggaggtattt tgcataggct cgcaaaatag
    21481 taatttcttg ccaggagagc cccgcggata acaccaattt attaaaacca tcgttttcac
    21541 aaataccaag attgatatta atcagtgcct cggcaaaaat attgttgacc tgggtaatat
    21601 tgaatgaatc agtttgagaa taggttacgt taaaatcact aatccagaat aatcgcccgt
    21661 cccgtgtttt gattttataa ggacgttcgg tataagtgcg cagccccatg ttttcaagca
    21721 taggcaagat gtcagatagg gggattgggg tctcataacg ataaagttta atgtgcaaag
    21781 gatattgcaa gtgagttttt tcataaaaat cgataaccag gggattatct ttggacagct
    21841 tttctatctg taaaatgtcc tggattgcct cttcgggagt atgttgttca caataactca
    21901 ttgacattgt ttcggtgtat ttatcaatga gctgtttccc ttttggggag ccaaattgtt
    21961 gagttaattg cttttgcaat ttatctttcc atgagtttgt catcattctt aaaattccat
    22021 gcaagatatg tttttatcgt tactcaaaag tataggccat ttcaaagaat aaattaaaat
    22081 ttgctaactt atctcgcaca aagtgaatca tttgttttca ggaaattatt aagtaagctt
    22141 taataaaatg ttaaggacat aaaattataa taaagcttcc ttcaaaagac gaagcaccta
    22201 ttatgtataa aaatatactg tttttttcaa attttagccg tatgcgttgg aacaagtatg
    22261 atggggatcg atatcaaagt ctttgggaac ataataagcg agatattaaa cacacatttt
    22321 tcggtacgat tgcaaacgga aaagatttgc gagaagtgct attccagcca gttactgctt
    22381 ctttttcgat gctagtcgcg ggggcatttg cacaattact ggtgccctat tccattttta
    22441 tgctctgtat gggtgaggct gggtttgcgg gcagaacatt aattaattat tttgaaataa
    22501 ctactgttgc tttggcaatg ccatttattg ctttattaag tactgtaggc cgatgtattg
    22561 caacggttaa atgcctggtt gaagatcaat acaatttggc tgagatggtt gaagacaaag
    22621 aggttaagcc aaataaaatc tatcttgatt gtgctgaagt aaactacact gtcagggatg
    22681 agaacaacaa tcagttcatc cgcaaatatt ataaattcca atataaaatg agaggtctca
    22741 acgatgagct ttgccaggga atctttactt tcagagattt agagcgtgct cctgataatg
    22801 acgatttcaa tggagatctt tccagtctca cactttataa atacgacatc ctggatttga
    22861 ttaaaagagt tcataaagat aaatttgaag aaatggaaga cgaaaatcta attcgtatat
    22921 ccatttaatt tattaattta gattggttag aaaatcaggc tgttttgggc tggaagctgt
    22981 aatggggagt gattattttt ctcaactact tgccacttaa gaagccacgt tatatcgtct
    23041 atgaatgata tgattggttc ctgcctatat tcttgatgcg ctgacttggt aatgagttat
    23101 tgagttggcg tctaaagcaa gacaaaacat agggatgaaa taaataaaag gattaaggga
    23161 ttaagagcgt gcgtttatct tatggtttgt taggtttcat gatatttaat ttggtggctt
    23221 gtgaaaaaat ggcattactg actacgccac ctaaaaaaca gcaattatct aatagtccac
    23281 tggctactcg ggcagagcga tatttttggc gaacccttca tgaggggcga tatcaggaca
    23341 ttccccaagc ggattatttg ctgatggcag cttacctcga gaatccgtat gattcgaaat
    23401 tagcggccca tcttggtttt attcatattt ggaaaattac ggaacgagaa agagtaaaaa
    23461 ataactctcc cttgcttccc aatgaaatta ttttgtctaa aaaatacttt gctgatgctc
    23521 tgcaactgga tccggaaaac ccaatttatc aaggatttta tggcgatacc caattagtag
    23581 aagggcagat ttttaaagac aaacaagaag aagtcagggg atactttact ttacagaaag
    23641 cgataagcaa ttggcctgaa tttaattatt ttactgcagg gtaccccatg agtagtttaa
    23701 gtgctgattc ggatcatttt aaaaagggtt tggattggca atggaaaact ttggatatct
    23761 gtgctggtga gaagattagt agacagaatc ctgattttac gccttatatg aagagggaaa
    23821 caacaatagg cgcgcaaaga gcctgttgga attcagaaat tgcgcctcat aattttgaag
    23881 gtttttttat gaatatgggg gatatgttag ttaaatccgg agatcccgaa acagggataa
    23941 aaatatacaa caatgccaaa ttggctaaaa attacaataa atggccgtat aaagacatgt
    24001 tggagaaacg tgttctgaat gtgaaagcca atgtgcaaca ttttaatcaa aaatccaatg
    24061 accccaatca gtcgattatg tttaattcgg gatatggatg cgtggtttgt catcaacggt
    24121 aagtaccatc tgattattct atatcaggtg acttgttaaa ttagtatgaa tgttagattt
    24181 cagcttaaaa cccccgcttg tgtttttgca aagcggtcag gataaaagcg aaggaaagtg
    24241 ttatagtttt aattcatttc ggcaaagaat ttaaaaaaaa catggaaaaa attactattt
    24301 atcataatcc tcgatgctca aaatctagac aagcattgga aatactgcaa aacaaaggtt
    24361 ttgaacccat tatcattgaa tatcttaaag ctcctcttga tctggaacaa ttgaaaagat
    24421 tacggactca cctggctctt gaagacttgg tgcgcacgaa tgaaaatgtt tttaaagaat
    24481 tgggtctgtc tctggctaat gaggcgcaag ttttagaagc tatggtaaaa gaacctatat
    24541 taatgcaacg cccaattgtc acatttaaag gtaaggctgc tatcgggcgc ccgccagaga
    24601 aaatcctgga gctgtttgat tagtctttat tgtacgataa tataaattta atcctgatac
    24661 catgactaat ctcactgtca tggtaaggga taaaagcatt tgcaaaactt cagagagaat
    24721 agctttttaa ttgtgatgca aattgcctta acacaagcag tccggactct tcggcctggc
    24781 tacaccattt ttgtattaaa ttaagaagat ccttattgga tagggacgtt tttagccata
    24841 tctgctgtaa taattcgcgg taattatgaa ccgcttgcaa gttgtcatgt tgcttaagta
    24901 atgttttcaa tcgcaactga tcagcatggg atagctgttc attcggtttc ttgagtaaat
    24961 ggcgagcatt ctttaaatcc cgatttttta tgcggtattg catatcgcgc agagtaggaa
    25021 atataactag cttgtaataa tcagatatca cttgaaagcg attgccaagt atggctttta
    25081 ccgaatctac atctatatga gatttattct tgtcaatgat cagttttggg ggtaattttt
    25141 ttactttggc aagctttaaa aaagacaaac aacgtatgta aaaccaaccc aaatcaaatt
    25201 cccaccattt aatggaaaat ttggctgagg aggcgaaggt atgatgatta ttatgcaatt
    25261 cttcaccagc aatcaataaa ccccagggaa aaacattggt tgaagaatct gacgattcat
    25321 gattgcgata accccaataa tgccctattc cattgaccac gccagcaaca agaggtatcc
    25381 acatcatttg gattgcccaa agggtaagtc caggaatccc aaacaagaaa agattaagtg
    25441 cgagcattaa aaatatgcct ttgttcgcat ggggagaata taatttgcgc tctatccagt
    25501 cgttgggggt accataggag tactttttaa tcatgtcttt atctttagag gcttctcgat
    25561 ataactctgg aacctgccaa aatacttttc ttatattaaa tacctgaggg ctatgcggat
    25621 ccccttccat gtcagtattg gcatgatgtt tccgatgaat ggctacccat tctgctgtgg
    25681 tcattccggt cgttaaccaa agccagaaac ggaaaaaatg gctgacaaga ggatgcagag
    25741 ttaatgcgcg gtgtgtctgg tagcgatgca ggtaaatggt aacagcagtg gtggtgatgt
    25801 gagtcaggac aaaaccggtc aaaagatatc cccatacagt catatttaaa aaaccaaaca
    25861 tcatttctcc ttttaggtgc cccaaattgt gtaattggca aaacgatatg taagaatatc
    25921 tctatatcca ctaagtatat actatttatt tttatcttag tggcatttaa aaagctaatt
    25981 atttacgcgc ttttattaaa agccgtcctg gattttctgc tgatattgcc cttatatccc
    26041 gcttttggcg gcgcaaattt ttgaattttg gctgagacta aatctgaaaa gttatgcaaa
    26101 aactcaaaat ccttaatttt aatgcgctcc aatttttgat tgattaattt ttctatagac
    26161 agcagcagct tgatttcttc tgtgctgacc aaggatacag ccaatccaga ggcgcctgcc
    26221 cgtcctgttc gtccaatccg atgaacgtag tcctcaggca catgaggcag gtcaaaattc
    26281 actacacagg caagtttctc gatgtctatg ccacgtgctg caatgtctgt tgcaattaat
    26341 gtttgtactt tgcctgactt gaaatcagcc agggcttttg ttcgttgagc ctgcgatttg
    26401 ttgccatgaa tagcaactga atatatttgg gattcggcca gttgcttaac cagtttattg
    26461 gctccatgtt tggttcgaga aaaaaccaaa gtttgtcccc agttattctt atgaagtaaa
    26521 tgactcagaa gggctaactt atggtttcca tctactgaat aaacagtttg ttttatggtt
    26581 tttaccgcgg tatttctcgg tgcgatatca atttcaaccg ctttattcag tatatttctg
    26641 gctaaagtac gtacctcagg agtaaaggtt gctgaaaaaa gcaggttctg cctttttaaa
    26701 ggtaataatt tgataatttt ttttatatca tgaataaacc ccatatccag cattctatcc
    26761 gcctcatcga gtaccagggt gtcaatctca tcaaatttaa cagcaccttg ctgatataaa
    26821 tccaataatc ttcccggggt tgcaacaagt atctcaaggc ctgagtctaa ttgtttaatt
    26881 tggttgtggc tttttactcc tccgtaaatc acagccgagc gcagtgttag gtatttgcca
    26941 tattggatta tgctctcgtg ggcctggatg gctaattctc gtgtgggcgt taaaatcagt
    27001 actttagctc gattacggct ggtttgagtt tgctgactgg ctttttgcaa gataggcaat
    27061 acaaagcttg ctgtttttcc tgttcctgtt tgagcggaac caagtaaatc atgcccattt
    27121 aaaatgagcg ggatggcttt taattgaata ctggtagggt tagtatagcc taactcacta
    27181 accgccctgt ttagcggctc aatcagcgct aattgtttaa aactcattgt tttccttatt
    27241 gatatctatt ggtattgtgg gctctgagtt taatcactca ttattttgtg gggcaaatga
    27301 ggcattaatt agaggggaaa aataggaatt ttcaaaggtt ttttgaaa
//
LOCUS       DAXLDT010000030        27299 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXLDT010000030 DAXLDT010000000
VERSION     DAXLDT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27299
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.030"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            36..1925
                     /locus_tag="ACF2SP_000997"
     CDS             36..1925
                     /locus_tag="ACF2SP_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HFD2358798.1"
                     /translation="MPSTRNIEKHNDSNPTLRALSLSALGIVYGDIGTSPLYTFKTVI
                     LLAGGGTPDVTTIMGSASLIIWTLIIIASVKYICFALRIDNDGEGGILALMSLLSLKL
                     KQKPFIIAVGLMGAALIYGDGTITPAISVLSAVEGLEILSPSLKYYVLPIAITILITL
                     FAIQSKGTTTIGKAFGPVMAFWFLTIGILGARGVIQHPFVLAAINPVYGLNFLFSNGA
                     TGFFILCGVFLCATGAEALYADLGHFGTAPIRCTWFGLAFPSLIFNYLGQAALVLEGA
                     STEHNIFYMLCPSDFLLPLIILSTVATIIASQAIITGAFSMTRQAMQLGWLPRLRVTQ
                     TSSEGYGQIYIGVVNWLLMLATLGLIIGFGSSEKLAAAYGIAVSATMLCTTVLLFIAL
                     HKLWKWNIITSGLVAGLFMIVDASFFAANLTKFINGGYIPITLAIIIYSMMYIWHKGY
                     KTIAIKQKEKNITVDSFLDSIQKEGVVRVSKTAVFLTSKEQDIPPTLVWHVKKNHVLQ
                     DKVIILNINNLSIPWCKPGDQLQIVETGAGIWHAVANYGFMEQPHIPKLLKKLEAQGY
                     DINIKDITYYIGHETIFVRNARHTLSKYIKILFVFMHRNALPMSNYFHLPPESVFEIG
                     RQIEI"
     gene            2143..3906
                     /locus_tag="ACF2SP_000998"
     CDS             2143..3906
                     /locus_tag="ACF2SP_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358799.1"
                     /translation="MVQFAKPSIGPPADCGRINLLIGRELGQYQNIIQKWIANLEDNA
                     KQKPSNISEFKFGKILGYLLLQSLKKANLHPDNFRSFGSDPWEEPHYQSARIAGKYVV
                     TQDTYRIYFDIPRIDHSDKEKIKANQDDAQILYFTTMTNDGLMVFSFEEKVEKIEPKL
                     FVELAKNIEVESNAWAQLIGFIEENFQYSQDVEFNPQVPQILGLILYATIFPEHRNEA
                     FNDLCPLLLKSENPNFNSITTKLLKDTIPDYETKAMAFLHKNNNPMRYKESDISKQGA
                     LLGLSSEKIDVVQNEMRERKALEVQNINRSQAIELKKTLIGAVDEYLRWRNNESKETD
                     YQKSSGAFTWLRHYTDFGKNRANDLKNELNKAQDLKTMLDVVQNHFANNSRLHNHSLD
                     SYLLRAMHKDFNKFNSIFNFEHLTIKNDTDANREWLREEMLGMAAKTNMNVTLEKSIN
                     SRQESPALPNKTKVHISIMKIPANEREENILAVHTVLRNDELLRNQDGSVPTIIKEIR
                     DIVGKIDPSEEENIANAIIEIKRKIADNSDHNSNENARDVIKAFESPSCCDFRKIRAA
                     LTANPAMDEIMNPVRVGVQLN"
     gene            4093..5241
                     /gene="nhaA"
                     /locus_tag="ACF2SP_000999"
     CDS             4093..5241
                     /gene="nhaA"
                     /locus_tag="ACF2SP_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFD2358800.1"
                     /translation="MNKSGSFYNLETIGGILLFIAAVIAIIIANSPFRVGYEYFLSIN
                     GSVSVGNLSITKPLLLWINDGLMAIYFLLIGLEIKREVNRGILSDKTNLLVPALTALA
                     GLIFPALIFIFFNAHHPVYLKGWAIPTATDIAFTLGIVSLLGSRVPFSLKILLTAIAI
                     FDDIAAIVIIALFYTEQLSLLSLSLALVFTLILIGLNYFKCRRISVFMLFGVALWIAV
                     LKSGVHATLAGIVIAMTIPDEGKESMLARLEDGLHHWVVFLILPLFAFANAGVSFVGL
                     DASMFTHPVVLGIGLGLFLGKQLGIFLSLGYFVQFKKFLKADKVNLAQVYGIALICGV
                     GFTMSLFIGSLAYQNYDLSLMPMVKIGVVLGSFIAGLTGFLVLKLKQQ"
     gene            5226..5339
                     /locus_tag="ACF2SP_001000"
                     /pseudo
     CDS             5226..5339
                     /locus_tag="ACF2SP_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655385.1"
                     /note="catalyzes the conversion of 3-hydroxyacyl-CoA to
                     3-oxyacyl-CoA; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
     gene            5465..6403
                     /locus_tag="ACF2SP_001001"
     CDS             5465..6403
                     /locus_tag="ACF2SP_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD2358801.1"
                     /translation="MHRFHNILFVSHGIKDETEVLQLTLRLAAESQAQLRILITCPPF
                     PDTLSEYKASYEEYLIDKMNKTIQLAKSGLGLSKKKIPIPIEVECGSMPDVRIIRHVL
                     KHSHDLLIKEAETLESRKGFKAVDMELLRKCPCALFLYRPLKNTLQDIRVAVAIDPKD
                     EEPVAQDLSLRLLEVSHSLNAFYQGKLRIVSCWDFVLENYLRDSVWLKISETELEKMV
                     VEEKHTHSLLLRELIKRSHISGESQIEHLKGHPEDAIPHLITHKKIDILVMGTVARTG
                     IAGFIIGNTAENILQKINCSLLALKPQGFVSPVKAY"
     gene            6421..9141
                     /locus_tag="ACF2SP_001002"
     CDS             6421..9141
                     /locus_tag="ACF2SP_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HFD2358802.1"
                     /translation="MVEQQNISEPDWHKQSVEKILVTLNSTEQGISDQESKKRLAYYG
                     FNRLPEPKRKSLFLRFLLQFHNILIYVLLGAVVVTSLLQHWVDTTVIIAVVIINALIG
                     FIQEGKAEKALEAIRNMLSPAATVLRNGERRRVNGEQLVPGDIIFLEAGDKVPADVRL
                     IKSHGLTVQEAILTGESNPVEKHIDSVPSDTVLGDRTCMAFSGTLVTNGQGKGIVVAT
                     GSSTQIGHISGLLSKVESLTTPLVIQIGLFAKWLTLFILLIAALLLAHGYFVQHHPFG
                     ELFMVVVSLSVAAIPEGLPAVLSITLAIGVQVMARRHTIVRRLPAIETLGSVSVICTD
                     KTGTLTLNEMAVSSVLTRDHLFILGGVGYEPQGTITLNEQVIDNNEYPLLKKLARAAM
                     LCNDASLHKQENNWTVEGDPMEGALLAFAGKAGLVLQEENALWVRTDVIPFDAKHRFM
                     ATLNHDHLQHAMIFVKGAPEQILKMCQNQQSNQGEIQPLNESYWKEQMEQVAARGQRL
                     LAFAVKKTKPEQTVLEFADIEAGLTLLGMVGLIDPPRPEAIEAVTQCHTAGIQVKMIT
                     GDHASTALAIGRQIGLKNLDKVLTGIDLDNMDGAILRNVVLETDIFARTSPEHKLRLV
                     MALQSHGMTVAMTGDGVNDAPALKRADAGIAMGRKGSEVAKESAEFVLIDDNFASIVA
                     AIREGRTVYDNLKKVISWTLPTNAGEAMTIILALLLGLSLPVTPIQILWINLITAVTL
                     GIALAFEPTEEGTMHRPPRPRSEPLLTGSLVWHIILVSILFICGVFGLYFYALDRGYS
                     IELARTIALNTLVVLEIFHLFYIRNIYSTSLTWKAVQGTKAVWIAVIVVTVSQFTITY
                     CSLLQTIFVTERVPFWDGVLIVLTGAVFFAVIEIEKQLRLRIGIIKRING"
     gene            9235..9798
                     /locus_tag="ACF2SP_001003"
     CDS             9235..9798
                     /locus_tag="ACF2SP_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358803.1"
                     /translation="MTIYFLFGKTGSGKSYIGELLRQLNIVHIDGDNHITQKMRDCLI
                     EDEQMTPEMIDEFVDVLIDVINTQKEKTPKESFAISQAMYLDKHRIKLLNAIPELKFV
                     MIDVTPVLRASRITNRFRNQESKVSLRYATKMDTFFEYPSHETIRLENNQGSDETLIE
                     QIREKLPALFNMDSKEELNPGIKLQFA"
     gene            complement(9913..10839)
                     /gene="leo"
                     /locus_tag="ACF2SP_001004"
     CDS             complement(9913..10839)
                     /gene="leo"
                     /locus_tag="ACF2SP_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease Leo"
                     /protein_id="HFD2358804.1"
                     /translation="MKNKIVLLAPVSLSFLFSCSTYAADYEFNGLSLSASLGILSGKA
                     HEYVYHPETDTKLSQLDWRIKNAPIIKGELNYDFLDWLSMNSRGWTTLVKNKAAMDDY
                     DWLNPYQDNWTHWSHHEDTYLNYANELDMSLRAWLMQKQNYKLGLATGYQWNSFDWKA
                     TGGCYQYYGGLYAGCFPGDQPGIGYQQKFRTPYVGLAGKYFINNFEFSGLLKFSDWVS
                     ARDHDEHYARNLTFNERGNSSRYYAATIHSGYFLKPNMKIFVEASYNHYSNGRADTEI
                     IDNDTGERSYFGDSAGLSNKHYTVALGLQYLF"
     gene            complement(10967..12574)
                     /locus_tag="ACF2SP_001005"
     CDS             complement(10967..12574)
                     /locus_tag="ACF2SP_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2358805.1"
                     /translation="MNKNLSALSLLWISITSMVGSGWLFGSLYSAHFAGPAAIIAWPL
                     AGFLLLFVALSYAELGTMFPQSDSLACLPLYTHGRLTGIIINVMTWFSLAILPVIETQ
                     GVIQYASNYIPDLVSHDALHYRNTPAGYMLSLVVLMSFVLFNYFGIGLFARINAAFTI
                     WKIIIPILTIASLATMSYHPGNFFQHGGFMPYGWQGVMAAMSSGGVLFSLLGFRQVVI
                     MMGEFEKPGQYIPLILGGSLVFVTLLYTGLQWSFIGSISEQALEKGWVNLSFTGDAGP
                     FAALAALVGMFWLSILLYTDAFISPYSTGLVYSTTAAHMLSTMGSVVPTPVILTQKNR
                     YQAPWVSLSVNFLLAATMSLVLHGWQEMSAFLVAILMISYSIGPVALVCLRQQLPHYK
                     RPFRLQGSSLIGFTGFYVCTVGVYWTGFHSVRKLLVITILGVVCSFLYCLIKNTHREI
                     DSKQGLWFIFYLFGLGIFSFLGNYGGKHILPQYWDLLYLLVFSLLVFGFALLSRKSSA
                     YTQNMVAQNTHTTITASAFSVERHDIA"
     gene            12905..15070
                     /gene="katG"
                     /locus_tag="ACF2SP_001006"
     CDS             12905..15070
                     /gene="katG"
                     /locus_tag="ACF2SP_001006"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFD2358806.1"
                     /translation="MDGKVGNTATGCPVIHGGMTSAETSNTAWWPNALNLDILHQHDT
                     KTNPMGKDFNYREEVKKLDFVAFKKDLHALMTDSQAWWPADWGHYGGLMIRMSWHAAG
                     SYRVADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKISWADLIVLAG
                     TIAYESMGLKTFGFGFGREDIWHPEKDVYWGSEQEWLGAKRYDDKDRQSLETPLAAVQ
                     MGLIYVNPEGVNGQPDPLRTAQDVRVTFGRMAMNDEETVALTAGGHTVGKCHGNGNAK
                     FLGPEPEAADIEDQGLGWINKTTRGIGRNTVSSGIEGAWTTHPTQWDNGYFYLLLNYD
                     WELKKSPAGAWQWEPIHIKEEDKPVDVEDPAIRHNPIMTDADMAIKMDPVYRKIAERF
                     YKDPDYFAEVFARAWFKLTHRDMGPKTRYIGPDVPKEDLIWQDPVPSGNRAYDIAAAK
                     AKIAASNLTIGEMVSTAWDSARTFRGSDKRGGANGARIRLKPQKDWEGNEPQRLTKVL
                     RILEGIAADTGASVADVIVLAGNVGIEKAAKAAGFDIIVPFAPGRGDATDDMTDAESF
                     DVLEPLHDGYRNWLKKAYDVRPEELMLDRTQLMGLTAHEMTVLVGGLRVLGTNHNNTQ
                     HGVFTDRVGVLTNDFFVNLTDMANVWIPSKDNLYEIRDRKAGNIKWTATRVDLVFGSN
                     SILRSYAEVYAQDDNKGKFIQDFVAAWTKVMNADRFDLA"
     gene            complement(15381..16658)
                     /locus_tag="ACF2SP_001007"
     CDS             complement(15381..16658)
                     /locus_tag="ACF2SP_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358807.1"
                     /translation="MKINKTTNDQNADQKSSQYIYVEKKEIGGIDTEIITESHYPIPW
                     GSASERPLDYYEKQSKIITYYKEDIDLDGNPFNKKIEIEIPKVDEGENRHRRIKVKVD
                     GNPIIFQDNNILDMDETFNIRDVNLLIHMVLNNFHPKSNSTCLQTITPFIHLPGTGGD
                     EDYEFNQKNNGDVSKVLLPYVQEMCSLNNSSVVRQLLIPVATKGGLHHWNLCMLFIDE
                     NNIPSLYYFESENLLASFDYYTYMYYMEDYKMHILPQVNALLAKFHYPAIDKVNFCQA
                     KQFSQEGCGIAMSLNMHKILEENYPQVICYSNTPRKGIPSHTEIRCLSSNVSIEEDAL
                     MRVELAFKIQACASIIAEGALDAKMVEKKSLLCSEILSDSRSLSVKAKEAFGFFQSAQ
                     PRISSEMHSVMAEDKGNDETSSSDINAEACRLW"
     gene            complement(17218..18450)
                     /locus_tag="ACF2SP_001008"
     CDS             complement(17218..18450)
                     /locus_tag="ACF2SP_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358808.1"
                     /translation="MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTAL
                     IATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPTYQEAG
                     ILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNTRHKGLAVSFITLGSICGLLLAII
                     VSKITLIFFSSQSPDFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPIL
                     NAWRYRKKAIAAIFLLAIYCNSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSM
                     GLVLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLQITVACSVLAVIISFF
                     IGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMV
                     MALLTLPALWLIQEKGTN"
     gene            complement(18491..19795)
                     /gene="sdbC"
                     /locus_tag="ACF2SP_001009"
     CDS             complement(18491..19795)
                     /gene="sdbC"
                     /locus_tag="ACF2SP_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbC"
                     /protein_id="HFD2358809.1"
                     /translation="MPNSSIQIWLQRQIALRTFPPNREDWYVKNGFGNSEMSLEERFA
                     CFVEAIRQEDSPYHEQLSGIDFHRFEIEVTLANQKTCMTEVIGFQNNSNQSSSLLNEK
                     VKAVGKGKHVIYFTGIGTQYQDCLIDIAKAVQITGAHYYAFEYPGMRKLGGEVLEVND
                     MVNTGIALANTLLEKGISIDDILFQGDSFGAAVAKKVSDQFKLQSNVTVRCILNNTFS
                     TFQAAVEGNLGAFSTLRYTVRPLLRYTGWDIRPGDNYGLDTPYQIHINHTGDLTLGAG
                     HATLAESVESNSQLEHFVDPCPEEYRIKRDIYKQWHWASLSEQGIRFLETKYGRNSDG
                     QLDTHLADLYLLKYDTGLGVYESFICKYLEDSNDYIANHPQKLSLEELPSPLESETVS
                     LGQLIVSVLPSRPSITGFFGRPKPVKDEVDAENQAQKSIELK"
     gene            20064..20948
                     /gene="legL6"
                     /locus_tag="ACF2SP_001010"
     CDS             20064..20948
                     /gene="legL6"
                     /locus_tag="ACF2SP_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL6"
                     /protein_id="HFD2358810.1"
                     /translation="MFYELILTRTSNLIQEFISIPHGVISLDLSLNELGNISNAELIQ
                     AFKYIPDSVISLDLTNNHLCDKSGAELAQLLAAIPARVTSLNLSCNDLHKKSGAELAQ
                     AFAAIPASVTSLNLHCNYLGNNRGVELAQAFAAIPENVTSLDLSMNYFDLESSADLSQ
                     IFTSIPPHVASLNLSFNSLHEVPFEKLVLFKDSLKHVQTVYLSFYSVKEMSKEQRRAL
                     GAAFPNAQKIILIDDYGHEIQPSIIISNLIRELSGKADAPSLLNQCILFTQRHQKDSD
                     NKIIPKELEESIRTFNSH"
     gene            complement(21069..21533)
                     /locus_tag="ACF2SP_001011"
     CDS             complement(21069..21533)
                     /locus_tag="ACF2SP_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFD2358811.1"
                     /translation="MKHRRSETQDIDDAIIKTLNKLLEIELAGVVRYTHYSFMVFGFN
                     RIPICKWMRDQAQESLNHAHLIGELITHFSYHPTLKIGKLLETHEHNISNILEESLEH
                     ERQSLDLYYQLLQYSENKSVLVEEFARQMIQEEEMHLGEISKMLNSPTTFKD"
     gene            21933..22292
                     /locus_tag="ACF2SP_001012"
     CDS             21933..22292
                     /locus_tag="ACF2SP_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358812.1"
                     /translation="MSNKAKNTHLKNGDSQTKQAYTNQASPLFFSQKQITPSSGTSET
                     KKTQEGKQPTQEISLETTTDILLAAILQELKTQNMIELMKLKAQQEQEQEELQAIETM
                     KEKDDARFNEIRQTMYA"
     gene            complement(22420..23490)
                     /locus_tag="ACF2SP_001013"
     CDS             complement(22420..23490)
                     /locus_tag="ACF2SP_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2358813.1"
                     /translation="MKTGRFDQARADAFAERLLSVLNDAALTLMISIGHRTGLFDVMD
                     ELDFASSEVIAEAAGCNERYVREWLNALVVGGIVDYDAHAKAYQLPAEHASFLTRKSS
                     PQNMAVVAQFIPVLAYVENGVVDAFKHGGGVPYEAYHRFHEVMAEESGQTILSSLTDK
                     ILPMVHGLVDKLNQGIQILDIGCGYGRALILMAKTYPQSQFTGYDLCDETIAHAVHSA
                     KENKIQNIHFEKMDLSVWDEQEKYDLITSFDVIHDQAKPAQVLSNVFKALKPRGIFLM
                     QDIKASSDVAGNVHHPLGALIYTISCMHCMTVSLSQNGLGLGAAWGEELAEKMLVEAG
                     FGKIEKHYLEHDIMNTYYVMRK"
     gene            complement(23860..25059)
                     /locus_tag="ACF2SP_001014"
     CDS             complement(23860..25059)
                     /locus_tag="ACF2SP_001014"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFD2358814.1"
                     /translation="MIKSFRVTIFLFLVSGLILARDMHAFPSGLESNQDKLVYFQLEA
                     ETFYSPKPVTIEGQFYLTYEVFLANVGDYLFSLENVEVLNGRKPYKVLFSYNQEELRK
                     MIYSFSGHSNHLTSDLTLRPGERGLLFFMVKFDSLDEIPEKIMHRFHLYSSKTKISEE
                     GVRYSFLAAPAYVKITKPLVIANPLDGKHWMAINGPSNHSIHRRARVAAHGIVYYPER
                     YAIDFMQFGVDGHVYKENPNKNENYYSYGANVYSVADGEVVAVYDGVPDNTPPGRDYP
                     VTLSNMAGNHVIIKIGKHQYALYAHLIPHSIKVTKGELVRKNQLIAKLGNSGNSDAPH
                     LHFHVIDKPSPLIGQGIPYAFEHFSTEEYRFAGDEWESPIVYEGRLTEHHHELVHENA
                     LMNFEEK"
     gene            complement(25114..25686)
                     /locus_tag="ACF2SP_001015"
     CDS             complement(25114..25686)
                     /locus_tag="ACF2SP_001015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2358815.1"
                     /translation="MALIRDSKSLVMSVFKFQPLGIKDLNQLYQWFQEPIINHWYARD
                     KSWSLDEITEKYEPRIAGMEHVPSFIVYKDDLPFGFVQFYLITDSLPDGIDGYESLLF
                     EKYNPRDLAGIDLFIAESEGRGRGLGVELINRFIDKFLTRFKAVVVDPDVNNQQAIRC
                     YEKAGFQQTGFSQDPNHLIMIKPLSLITGN"
     gene            complement(25876..27222)
                     /locus_tag="ACF2SP_001016"
     CDS             complement(25876..27222)
                     /locus_tag="ACF2SP_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFD2358816.1"
                     /translation="MRKWIISLLLLVIIVLCSYPIIGMMALSTVKKNVDNIPKSPFLT
                     WQLKDNQSGWFCSTAVLNLLVDLPAQENKDAQGNIKIQPALHLSMDFPVIVRHGPFIM
                     TNNGLRFGLAWVTTKPETHYGALINYLNQTAVSYSLPSINMQGTNNGDQGKFVFVWQG
                     VSASLKVNPRIDKASGHVTMYGMSTQVQETDVRIGKVKVNFALNRYMDDLWLGDSSIM
                     LSSLSIITNSQPVFDLQNLGMDAWAGVNSNLLDFNLQLSLNKFVMNNNSYGPGQLKIE
                     VKNLDPQAMAKLNHLNYDYPSNQIAILAEMFNLLSKGATLNLNQELDTPQGHLSSSLN
                     LALTKSSFSNPEDLLQNLKGNGQFKAPIKLVEQLLFESIKSKTATPAADMSQSQVTEP
                     LVDADAEAHKQTEELLQKLVSKGIIKVEGDYYQASFTLENTKFTVNGQPFDPALLN"
BASE COUNT         8470 a         5532 c         5316 g         7981 t
ORIGIN      
        1 tgagtaaagc caaacttaat aagagagcca actcaatgcc ctcaacaaga aacattgaaa
       61 agcataacga ctccaatcct acattgcgag cactaagtct atcagccttg ggcatagttt
      121 acggagatat tggaacaagt cctctttata cttttaaaac ggtgatattg ctggctggag
      181 gaggaactcc agacgtaact accattatgg gttcggcatc attgattata tggacactga
      241 taatcatcgc atctgttaaa tatatttgct ttgctttacg gattgacaac gatggagagg
      301 gaggcattct cgcgttgatg tctcttttaa gtttaaaact aaaacaaaag cctttcatta
      361 ttgcggttgg acttatgggg gctgctctta tctatggaga tggcacaatt acccctgcta
      421 tatcggtact ctctgctgta gagggacttg agatcctttc tccatcactt aagtattatg
      481 ttttacctat tgcgatcaca attctgataa cactttttgc gattcaatct aaaggtacca
      541 caactattgg aaaggccttt ggcccagtga tggcattttg gtttttaacc attggtattt
      601 taggggcaag aggagttatc caacaccctt ttgtcttagc ggcgataaac cctgtttatg
      661 gcttaaattt tttattttca aatggcgcaa caggattttt tattctatgt ggagtgtttc
      721 tttgtgccac tggtgcagag gctttatacg cagacctggg acattttggt accgccccaa
      781 ttcgctgcac ctggtttggt ctggcttttc caagccttat ctttaactat ctgggacaag
      841 ccgctttggt tttggaagga gcatctacag agcataatat tttttacatg ctgtgcccaa
      901 gtgacttttt attaccgtta ataattctgt ctaccgtcgc aacaattatt gccagccaag
      961 ccattattac cggggcattt tccatgacaa gacaagcaat gcaactgggc tggctaccca
     1021 ggttacgagt cacgcaaacg tcttcagagg gttatgggca aatttatata ggagttgtta
     1081 actggctttt aatgctagct acccttgggc ttattattgg atttgggagt tctgaaaaat
     1141 tggctgctgc ttatggcatt gctgtttcag cgactatgct ttgcaccact gtgcttttat
     1201 ttattgcact gcataaacta tggaaatgga atattatcac atcagggctt gttgcgggat
     1261 tattcatgat tgtcgatgcc tctttttttg cggccaatct cacgaaattt atcaatggcg
     1321 gatatatacc aatcacattg gcaatcataa tctattccat gatgtatatt tggcataaag
     1381 gctataaaac catcgcgatc aagcaaaaag aaaaaaatat aacagtcgat tcctttctgg
     1441 acagtattca aaaagagggg gtagtccgtg tgtctaaaac tgctgttttt cttacatcca
     1501 aggagcagga tattcctcca accctggttt ggcatgtaaa gaaaaatcat gtgttgcagg
     1561 ataaggttat tattttaaat ataaacaact tgtccattcc ctggtgtaaa cctggtgatc
     1621 aattacaaat tgtagaaaca ggagcaggca tctggcatgc cgtagcaaat tatggcttta
     1681 tggaacaacc tcatattcca aaattattaa aaaaattaga ggctcaaggc tatgacatca
     1741 atatcaaaga cataacctat tatatcggtc atgaaacaat atttgtccgc aatgcccgtc
     1801 atactttgtc aaaatacatt aaaattctct tcgtttttat gcatcgcaat gcccttccca
     1861 tgagtaatta ctttcatctt ccgccggaat cagtatttga aataggacga cagatagaaa
     1921 tttagtgaaa ataattgctc cctgacatgg aaaatctcag taggcgagca cagttttatg
     1981 atcagggttg aattttgcac ctgatcttat agcacatgtg acaaatcaaa attaaatcct
     2041 ggcttttgct cttttgcaga ttatacccag gtcctgaaaa agattaaaaa cataccaaaa
     2101 aagtggccta taattataaa acaaatactt tggatgatag aaatggttca atttgccaaa
     2161 ccatctattg gccctccggc tgattgtgga cgaataaatc tcttgattgg acgtgagtta
     2221 gggcaatatc aaaatatcat tcaaaaatgg attgctaatc ttgaagacaa cgcgaaacaa
     2281 aaaccttcta atatttctga atttaaattt ggaaaaattt taggatatct tttacttcaa
     2341 agcttaaaga aagcaaattt acacccagac aacttcagat cttttggttc agacccttgg
     2401 gaagaacctc attatcaatc ggcacgaatc gctggaaaat atgtggtaac tcaagatacg
     2461 taccgaattt actttgatat accaagaatt gatcattcag ataaggaaaa aataaaggcc
     2521 aatcaagacg atgcgcagat actttatttc acaacaatga ccaatgatgg ccttatggtt
     2581 ttttcctttg aagagaaagt agagaaaata gagcctaaat tatttgtgga actggcaaaa
     2641 aacatagagg tagaatcaaa tgcatgggca caattaattg gctttataga agaaaacttc
     2701 caatactctc aagatgtgga gttcaatcca caagtccctc aaatattagg tctgatttta
     2761 tatgcaacta tttttcccga acacaggaac gaggctttta atgacctgtg tccattgctt
     2821 ttaaaatcag agaaccctaa ctttaactca attacaacaa aactgcttaa agacaccatt
     2881 cctgattatg aaaccaaagc gatggcattc ctgcataaaa ataacaatcc gatgcgttat
     2941 aaggaaagcg atatcagcaa acaaggagcc cttttaggtc tttcgagcga aaaaattgac
     3001 gtagtacaaa acgaaatgag agaacgtaaa gcattagaag tacaaaacat taatcgatca
     3061 caagccatcg aactaaaaaa aactctcatt ggtgccgtgg atgaatacct tagatggcgc
     3121 aataatgaaa gcaaagaaac ggattatcaa aaaagttccg gtgcttttac ctggctgcgt
     3181 cattatactg attttggcaa aaaccgagcc aatgatttaa aaaatgagct taataaagca
     3241 caagatttaa agactatgct tgatgttgtc caaaatcatt tcgcaaataa ctcccgatta
     3301 cataatcatt ccctggatag ctacctgcta agagccatgc ataaagattt taataaattc
     3361 aattcaatat ttaattttga acatcttaca attaaaaatg ataccgatgc taaccgtgaa
     3421 tggctaagag aagaaatgtt gggaatggca gcaaaaacga acatgaatgt tacattagaa
     3481 aagtcaataa atagtagaca agagagcccc gctctgccta ataaaacaaa agtccatatc
     3541 agtatcatga agattcctgc taacgaacga gaggaaaata ttttggcggt ccatacagta
     3601 ttaagaaatg atgaattgct gagaaatcaa gatggatctg ttccaactat cataaaggaa
     3661 atacgagata ttgtagggaa aatcgaccct tcagaagaag aaaacatagc caatgcgatt
     3721 attgaaatca aaagaaaaat agctgacaac agcgaccata attccaacga gaatgcgcgc
     3781 gacgtcatca aagcgtttga aagtccgagt tgttgtgatt ttagaaaaat ccgcgctgcc
     3841 ttaacggcca accctgcaat ggacgaaatc atgaatcctg tcagagtggg agtgcagtta
     3901 aactaactca attcacataa atgtcatttt taccttctga atctttccca cgcccttccc
     3961 aatatgctac gcacaaaatc ccggtatatt acacttgctt tttattttta gaagtattaa
     4021 tcaagaataa aaagttgtat gaagctatag cacaaagtac acttaatttt tagccactcg
     4081 tgaactaatt taatgaataa atcgggttct ttctacaatc tggaaactat agggggtatt
     4141 ctcttattta ttgctgctgt gattgcgata atcatagcca actcgccttt ccgggttggg
     4201 tatgaatact ttttaagcat caatggcagt gtttctgtgg gaaatttgtc tataacaaaa
     4261 cccctgcttt tatggatcaa tgatggatta atggccattt atttcctgtt gattggtttg
     4321 gaaataaaac gggaagttaa tcgaggcatt ttaagtgaca aaacaaatct tttggtgcct
     4381 gcccttacgg cgttagccgg attgatattc cccgcattaa tatttatttt ctttaatgct
     4441 caccatcctg tgtatctgaa aggttgggca attcctaccg ctactgatat tgcctttacc
     4501 cttggaattg tctctctttt aggctcacgc gtacctttct cactcaagat tttgctgacc
     4561 gccattgcca tttttgatga tattgcagct attgtcatta ttgcattatt ttataccgaa
     4621 caattatcgt tgctctctct ttcacttgct ttggtattta ccctgatttt gattggctta
     4681 aattatttta aatgtcgtcg tatttcagtc ttcatgctgt tcggggtagc tctatggatt
     4741 gccgttttaa aatccggtgt tcatgccact cttgcaggga ttgtgattgc catgacgatt
     4801 cctgatgagg gtaaggaatc catgctggcc cgtttggaag acggtttaca tcactgggtt
     4861 gtgtttttaa ttctcccctt atttgctttt gccaatgcag gggttagctt tgtcggactt
     4921 gatgcgtcca tgtttactca tcccgttgtt ttagggattg gattaggatt atttcttggc
     4981 aagcaattgg gtattttctt gtctttgggt tattttgttc aatttaaaaa atttcttaaa
     5041 gccgataaag tgaatcttgc ccaggtttat ggcattgcgc tcatttgtgg ggtgggcttt
     5101 accatgagtt tgtttattgg ctctttggct taccaaaatt acgatttaag tttaatgcca
     5161 atggttaaaa ttggtgtagt acttggttct tttatagcgg gattaaccgg atttttagtg
     5221 ttaaaattga aacagcaatg attcgcaaaa tgcgcaaaaa cgttttaaat actataattt
     5281 cagcaatacc ggttggacgc ttgaacctcc cgaaaaatat tgcagaagcc gttgcctttt
     5341 gatattacct gcatcggccc tatcacgagg gcaagctgga tgtcaatggc attcaaacca
     5401 gggagcaatc atgattcaaa tacggatttg aaagattgcc cctttctttc aaggaggaga
     5461 agtgatgcat cgatttcaca atatcttatt tgtcagccat ggaatcaagg acgaaaccga
     5521 ggtgttgcaa ttgacattga gactagctgc tgaaagtcag gcgcagcttc gcatcttaat
     5581 tacttgccct cctttcccgg atacactgag tgagtacaaa gccagctatg aagagtactt
     5641 gattgacaag atgaacaaaa ccattcaatt ggccaaatca ggacttggtt tatctaaaaa
     5701 gaaaatcccc atacctattg aagtcgagtg tggcagtatg cccgatgtcc gaattattcg
     5761 ccatgtcctt aaacactctc atgaccttct cattaaagaa gcggaaacac tagaaagcag
     5821 aaaaggtttt aaagcagtag atatggaatt attacgcaaa tgcccttgcg ccttgtttct
     5881 ttatcgccct ctaaagaaca cgctgcaaga cattcgagtg gcagtagcaa tcgaccccaa
     5941 agacgaagaa ccagttgcac aagatttatc acttcgcttg ctggaagtat ctcattcatt
     6001 gaatgccttt tatcaaggca aattacgcat tgtttcatgt tgggattttg tactggaaaa
     6061 ttatttgcgt gatagcgttt ggctaaaaat ctctgaaacc gaacttgaga aaatggtcgt
     6121 tgaagaaaaa catactcata gtttactcct aagagagctg atcaaacgct ctcacatttc
     6181 tggagaatcc caaattgagc atctgaaagg acatccggag gatgctatcc cccaccttat
     6241 aacccataaa aaaatcgata ttttagtcat gggtacggtc gcccgaaccg gaatagcggg
     6301 atttattatt ggcaatacgg cggaaaatat attgcagaaa attaattgtt ccctattggc
     6361 attaaaacca caaggttttg tttcccctgt caaagcgtat taatccggtg gaaaagaata
     6421 atggtggaac aacaaaatat ttccgaacca gactggcata agcaatcagt agaaaaaata
     6481 cttgttacgc taaactccac cgagcagggg ataagtgatc aagaatctaa aaagcgatta
     6541 gcgtattacg gtttcaatcg cttgccagag ccgaaaagaa aaagtctttt tttacgcttc
     6601 ttgctccagt ttcataatat acttatctat gtattattag gtgcggttgt ggtgacctct
     6661 ctcctgcaac actgggtaga taccactgtt ataatagctg ttgttatcat caatgccctg
     6721 atcggtttta ttcaggaagg aaaagcagaa aaagcgctgg aagcgattcg aaacatgttg
     6781 tctcctgctg ccactgtatt acgcaatggg gagagacggc gtgttaacgg tgaacaactt
     6841 gtgccaggag atattatttt tctggaagca ggagacaaag ttcctgctga cgtacgctta
     6901 attaaatctc atggtttgac ggtccaggaa gccatcttga ctggcgaatc caatccagtt
     6961 gaaaaacaca ttgactctgt cccatccgat acggtattag gtgaccggac ttgcatggca
     7021 tttagtggaa ccttggtaac caatggccag ggcaaaggca ttgttgtagc aacaggctca
     7081 tccactcaaa ttggacatat tagtggttta ttatcaaagg tagagtcact cactacacca
     7141 ttagtcatac aaattgggct gtttgcaaaa tggctcactt tattcattct gttgattgcc
     7201 gcactgcttc ttgcacatgg ttattttgtt caacatcatc ctttcggtga attgtttatg
     7261 gtcgtggtta gtttatcggt tgcggctatt ccagaaggcc ttcctgccgt tctttcgatt
     7321 actttagcta ttggtgtaca ggtcatggca cgacgacata cgattgtacg ccgattacct
     7381 gctattgaaa ccttgggctc cgtatcggtt atctgtactg ataaaaccgg taccctgact
     7441 ctgaacgaaa tggccgtttc ttctgttctg actcgtgatc atctatttat tttgggtggt
     7501 gtcggttatg agcctcaagg aacaattacc ctaaacgaac aagtcattga taacaatgaa
     7561 tatcctttac ttaagaagct cgcacgtgcc gctatgcttt gtaatgatgc ctcacttcac
     7621 aaacaggaaa acaactggac tgtagaagga gaccccatgg agggcgcctt actggcattt
     7681 gccggtaaag caggattggt tctgcaagaa gaaaatgcct tgtgggtacg taccgacgtg
     7741 attcctttcg atgccaagca tcggttcatg gcgaccttaa atcatgatca tttgcagcat
     7801 gcgatgatct ttgttaaagg tgctcctgag caaattttga agatgtgtca aaatcaacaa
     7861 agcaatcaag gagaaattca gcctttaaat gagtcatatt ggaaggaaca aatggagcag
     7921 gttgccgcaa gaggacaaag attactagcc tttgcggtta aaaaaacaaa accggaacaa
     7981 accgttttgg aatttgccga tatagaagct ggtttgacac tacttgggat ggttgggtta
     8041 attgatcctc caagacctga agccattgag gctgtgacgc aatgccatac agcaggcatt
     8101 caggttaaaa tgattactgg agatcatgcc agcacggcac tggccattgg tcgccaaata
     8161 ggacttaaaa acctggataa agtattaacg ggtattgatt tggataacat ggatggtgcc
     8221 atactcagaa atgttgtgtt ggaaaccgac atttttgccc gaaccagtcc agaacataag
     8281 ctaaggctgg tcatggcatt gcaatcccat gggatgactg ttgccatgac tggtgatggt
     8341 gtgaatgacg ctcctgcgtt aaaacgagca gacgcaggaa ttgccatggg gagaaaaggc
     8401 agcgaagtag ctaaagaatc tgcggagttt gtactgatag atgataattt cgcctcaatt
     8461 gtcgcagcca ttcgtgaagg tcgtactgtt tatgacaacc ttaaaaaggt catcagttgg
     8521 actttaccta ccaatgcggg tgaggcaatg accattattt tagcgttgct tttaggactc
     8581 agtttaccag tgacgccaat tcagatttta tggattaatt taatcaccgc agtcacgcta
     8641 gggattgcgc tggcttttga accaacggaa gaaggcacca tgcatcgtcc accgcgccct
     8701 cgaagtgaac ctctgttaac tggcagttta gtatggcaca ttattctggt ttccattcta
     8761 ttcatttgtg gggtttttgg actctatttc tatgctctcg atcgcggtta ctcgattgaa
     8821 ctggcccgca caatcgcttt gaatacatta gttgtgcttg aaatttttca tcttttttat
     8881 attcgtaata tttatagcac ctcacttaca tggaaagcag tccagggaac aaaagcagtc
     8941 tggattgctg tcattgtggt tactgtatca cagtttacaa tcacctattg ctctcttttg
     9001 caaacaatat ttgttactga acgtgtccca ttctgggatg gagtattaat cgttctaact
     9061 ggagcggtat ttttcgctgt aattgaaata gaaaagcaac ttcgattgag gataggtata
     9121 ataaaacgta tcaatggtta ggtaataaat aataaagtac acatttccat gattttaagg
     9181 tatggttatt cactcaccta atgtgaaaaa catacattac aagggttgtg aacaatgacc
     9241 atctattttt tgtttggcaa aactggctcg ggtaaaagtt atattggaga gctgctcaga
     9301 cagcttaaca tcgtccatat cgatggtgat aaccacatca cccaaaaaat gcgtgactgt
     9361 ttaatcgaag atgaacaaat gactcctgag atgattgatg aatttgtcga tgtcttgata
     9421 gatgttatta atactcaaaa agaaaaaaca cccaaagaga gttttgctat ttcgcaagcg
     9481 atgtatctcg acaagcatcg tatcaagtta ttaaacgcca tacccgaact caaatttgta
     9541 atgattgatg tcacgcctgt actgcgggca agtcgcatca ccaaccgatt tcgcaaccag
     9601 gaaagcaaag taagcttgcg ctacgccacc aaaatggata cattttttga gtatccttca
     9661 catgaaacaa tccggcttga aaacaatcaa ggctcagatg aaacgcttat agagcaaatc
     9721 cgtgaaaaat tgccggcact ttttaacatg gattcaaaag aagaattaaa cccaggcata
     9781 aagcttcagt ttgcataata ttcagatcca tatccagata tggttgaaat aaagagctta
     9841 tgtagcaact gtcttgaagt tagaacttaa gacagttgct acttgccagt tgagattaga
     9901 gaatttgacc ttttaaaaaa gatattgtaa cccaagggca acagtatagt gtttattgga
     9961 tagacctgct gaatcaccga aataactgcg ctcacccgtg tcattgtcaa tgatttcagt
    10021 atccgctctc ccattagaat aatgattgta agaggcctct acaaaaattt tcatattagg
    10081 tttcaaaaag tagccagaat gaatcgttgc cgcataatat ctggagctat ttcctcgctc
    10141 attgaatgtt aagttgcggg cataatgctc atcatgatct cgagctgaaa cccaatcact
    10201 aaattttaaa aggccactaa actcaaaatt attaataaaa tacttccctg ccagaccaac
    10261 ataaggagtt ctaaattttt gttggtatcc tattccaggt tgatctcctg gaaaacaacc
    10321 ggcatagagc cctccgtaat attggtaaca acccccggta gctttccagt caaaggaatt
    10381 ccattgataa ccagtagcca ggcctaactt gtaattttgt ttttgcatca accaagccct
    10441 taagctcata tctaactcat ttgcataatt gagatacgta tcttcatgat gagaccagtg
    10501 agtccagtta tcttgataag gattgagcca atcataatca tccatagcag ctttattttt
    10561 taccaaagtt gtccagcctc tactgttcat gctaagccaa tccaggaaat cataatttaa
    10621 ttcccctttt attatcggag cattttttat gcgccaatcc agctggctta acttagtgtc
    10681 tgtctctgga tggtaaacat attcatgagc tttacctgac agaatgccta gagaagcact
    10741 taatgacaaa ccattaaatt cgtaatccgc agcataagta gagcatgaga aaagaaagga
    10801 taaagagact ggagccaaca aaactatttt atttttcata ttttttccta gttaaagctg
    10861 gcaatccttg cagcaaagtg actttaaaaa atcaatcgca ttattgtgat ccaaaatgat
    10921 cttgtcaaga tcaatattaa ataatattga tcattaagga ctctaatcaa gcaatatcat
    10981 gtcgctcaac tgagaaagca gaagcagtta tagtggtgtg tgtattttga gcaaccatgt
    11041 tttgagtgta ggcactggac tttctggaca atagggcaaa accaaataca agcaagctga
    11101 aaactaataa atacaacaaa tcccaatact ggggaaggat atgcttacca ccataattac
    11161 caagaaatga gaaaatacca agtccgaaaa gatagaatat aaaccatagt ccttgtttgc
    11221 tatcaatctc ccgatgtgta ttttttataa ggcaatagag aaaagaacac actactccca
    11281 gaatagtgat caccaataac tttcgtacgc tatggaaacc tgtccaataa acaccaactg
    11341 tacaaacata aaatccggta aaaccaataa ggctactccc ctgcaaacga aaaggacgct
    11401 tatagtgcgg taattgctga cgtaaacaaa ccagtgctac aggtcctatg gaatagctaa
    11461 tcattaatat tgctacaaga aaagccgaca tttcctgcca tccgtgcaac acgagcgaca
    11521 tggtggcagc aagtaagaaa tttacgctta aactaaccca aggagcttga tacctgtttt
    11581 tttgggtaag aattacaggt gtaggaacaa ctgagcccat agtgcttagc atgtgagctg
    11641 ctgtggttga ataaacaaga ccagtggaat aaggtgatat aaaagcatcg gtgtacaata
    11701 aaatactcaa ccaaaacata ccaaccagtg cagccagagc ggcaaatggt cctgcatcac
    11761 cggtaaaaga caaattcacc cacccttttt ccagcgcttg ttcgcttata ctcccaataa
    11821 aggaccattg taaaccagtg taaagtagcg tcacgaacac tagtgaacca cccaaaatca
    11881 ggggtatgta ttgtcctggt ttctcaaatt cacccatcat gataaccact tgtctaaacc
    11941 ctaaaagtga aaaaagcaca cctccgcttg acattgctgc cataacgccc tgccagccat
    12001 aaggcataaa accaccgtgc tgaaaaaaat tacccgggtg ataactcatg gttgccaagc
    12061 ttgcgatagt aaggataggg attattatct tccagatagt aaatgcagca ttaatgcggg
    12121 caaacaaacc aataccaaaa taattaaaca aaacaaagct cattaataca accagggaca
    12181 gcatataacc tgctggagta tttcgataat gcaaagcgtc atgagatact agatctggaa
    12241 tgtaattact ggcatattga atgacacctt gcgtttcaat cactggcaaa atagccaggg
    12301 aaaaccaggt catgacatta ataataatcc cagtcaaacg tccatgagta tataaaggca
    12361 agcaagccag gctgtcagac tgtggaaaca tcgtacctaa ttcagcgtaa gacaaggcaa
    12421 caaacagaag taaaaaacct gccagtggcc aagcgataat ggcagcagga cctgcaaaat
    12481 gagcagaata taaagaacca aataaccagc ctgagcctac catgctggta attgaaatcc
    12541 aaagtaaact caaagcactt aaatttttat tcatctgatt ttcacatgtc cttatgattt
    12601 tgatgcaggc aaacaattcg ccagtcagca gagcgaaatc attatatttt tgcattgtaa
    12661 acagaaatat aatgactacc aaccctataa gagtcaagtt taaccaggta ggttacgaat
    12721 accttccagc atcaggaaaa atgaaaaaca agaactcgca gcagtgattt tctttgctat
    12781 ggattcggca tttttcactc tgcaatctct cgcttgaagt atctctgctt ccgattgctt
    12841 agctgccctc ccttaagcta tagttaggtc tctagtgaat tatgatctgc gaccaggagt
    12901 gtctatggat ggaaaagttg ggaatacagc aaccgggtgt ccggtgatac atggcgggat
    12961 gacatctgca gaaacatcca acacagcatg gtggcccaat gccttgaacc tggatatatt
    13021 gcatcagcat gacactaaga caaatccaat gggaaaagat ttcaactatc gtgaagaagt
    13081 caagaaactg gattttgtgg cattcaaaaa ggatttgcat gctttgatga ctgacagcca
    13141 ggcatggtgg cctgctgatt gggggcacta cggtggttta atgattcgta tgtcctggca
    13201 tgctgcaggt tcttatcgtg ttgcggatgg ccgcggcggt gctggtacag gcaatcagcg
    13261 ctttgcccca ttgaattcct ggcctgacaa tgtgaacctg gataaagcac gtcgcttatt
    13321 atggcccatc aagaaaaaat atggcaacaa gattagttgg gcagacttga ttgtcctggc
    13381 tggcaccatc gcttatgaat caatgggact taaaactttc ggttttggtt ttggccgcga
    13441 agacatttgg catcccgaaa aagatgttta ttggggttca gaacaagaat ggttaggcgc
    13501 caagcgttat gatgataaag accgtcaatc cctggaaacc ccgctagcgg cagtacaaat
    13561 gggattaatt tatgtaaacc ctgaaggagt gaatggtcaa cctgacccgc tgcgcacagc
    13621 gcaggatgtt cgtgtaacct ttgggcgtat ggctatgaac gacgaggaaa ccgttgctct
    13681 gactgctgga ggacatactg ttggcaaatg tcacggtaat ggcaatgcaa aatttttggg
    13741 accagagcct gaggcagctg atattgagga ccaagggctt ggctggatta acaaaaccac
    13801 cagaggcata ggccgtaata ctgtttcaag tggtattgaa ggcgcatgga caacacaccc
    13861 tacccagtgg gacaatggct acttctattt attgctgaat tacgattggg aactgaaaaa
    13921 aagccccgcc ggtgcctggc agtgggaacc catccatatc aaggaagagg acaaaccagt
    13981 agatgtcgaa gaccccgcca ttcgacacaa tccaatcatg acagacgctg acatggcaat
    14041 caaaatggat cccgtttatc gtaaaatcgc cgagcgcttt tacaaagatc ccgactattt
    14101 tgcagaagtt tttgcacgag cctggttcaa actgacccat cgcgatatgg ggccgaagac
    14161 gcgctacatt ggccctgatg ttcctaaaga agatttgatt tggcaagatc cggtgccttc
    14221 tggtaacagg gcatatgata ttgctgccgc caaagcaaaa attgcggcca gcaatctgac
    14281 catcggtgaa atggtttcta ctgcatggga cagcgcgcgc actttccgcg gctcagataa
    14341 gcgcggtggt gcgaatggag cacgtatacg actaaagcca caaaaagact gggaaggcaa
    14401 tgagccacaa cgcctgacta aagtcttgcg aattcttgaa ggcatcgctg ctgatacagg
    14461 cgcaagcgtg gcagacgtga ttgtacttgc cggaaatgtc ggtatcgaga aggctgccaa
    14521 ggcagctggc tttgacatta ttgttccctt tgctcctggc cgtggggatg caaccgatga
    14581 catgacggat gcagaatcct ttgacgttct ggaaccgctt catgatggct atcgcaactg
    14641 gcttaagaag gcatatgatg tccgtcctga agagctcatg ctagatcgca ctcagctcat
    14701 gggactgaca gcccatgaga tgacagtcct tgttggaggt ctgcgcgtgt tgggtactaa
    14761 tcacaacaat acccaacatg gtgtattcac tgatcgcgtt ggtgtcttga ctaatgactt
    14821 ttttgtcaac ttgaccgata tggccaatgt atggatacca agcaaagaca atttgtacga
    14881 aattcgagac cgtaaagcgg gtaacatcaa gtggacagcg actcgtgtag atctggtttt
    14941 tggctctaat tcgattctgc gctcctatgc tgaagtctat gcccaggatg ataacaaggg
    15001 aaaattcata caggactttg ttgcagcatg gacaaaggtt atgaatgctg atcgctttga
    15061 tttggcataa tcctttgcaa gttgtcacac catagacaat catagtagaa attgttccga
    15121 ttatgtttag ctttgattgc cttttatcac atcaaagcta aacatagcgt actaccataa
    15181 gatgttgttt caaaagcgaa catttacgat ggtttgtatt aggcctgatt tgcctaactt
    15241 gtctcttttg ttttatttca gcgttgcaaa cgtttctgta agtctcatct actgcatgta
    15301 aaccccactc ttcaaaacgt ttacgccttg ccctaaaaca aaataatcag atcatgcaag
    15361 aggtctattt atattatgtc tcaccacaaa cgacatgctt cagcatttat atcactggaa
    15421 gatgtttcat cattgccttt gtcttctgcc ataaccgaat gcatttcaga cgatattcta
    15481 ggttgtgctg attgaaagaa accaaatgct tctttagcct ttacacttaa actacgtgaa
    15541 tcacttaaaa tttcgctaca aagcaagctc tttttttcta ccatctttgc atcaagagcg
    15601 ccctctgcaa taattgatgc gcatgcttgt attttaaatg caagctctac tcgcattaaa
    15661 gcatcctctt caatactcac attacttgat aaacatcgta tttctgtatg acttggaatc
    15721 ccttttctcg gtgtattgga ataacaaata acttgaggat agttttcttc cagaattttg
    15781 tgcatattaa ggctcattgc aataccacaa ccctcctggc taaattgttt tgcttgacag
    15841 aaattgactt tatcaatggc tggatagtga aatttagcca ataacgcatt gacctgtggg
    15901 aggatatgca ttttgtaatc ttccatgtaa tacatataag tatagtaatc aaacgaagct
    15961 aataggtttt ctgattcaaa ataataaagt gaaggaatat tattttcgtc gataaataac
    16021 atacacaaat tccagtgatg aagaccacct tttgttgcca ctgggattaa tagctgccta
    16081 accacagaac tgttgtttaa cgaacacatt tcctggacat atggaagaag aactttactc
    16141 acatctccat tatttttttg attaaattca taatcctcat ctccaccagt tccaggtaga
    16201 tgaataaaag gagtgattgt ttgcaaacag gttgagttac tcttgggatg aaaattgtta
    16261 agaaccatat ggattaacaa atttacatca cggatattaa aagtttcatc catatccaga
    16321 atattgttat cctgaaaaat tatgggattt ccatcaacct ttactttaat tcttcggtgt
    16381 ctgttttcac cttcatcaac tttaggtatt tctatttcaa tttttttgtt aaatggatta
    16441 ccgtccagat caatatcttc tttatagtag gtgataattt ttgattgttt ttcataataa
    16501 tcgagcggtc tttctgaagc agagccccaa ggaatagggt agtgagattc ggtaataatt
    16561 tctgtatcaa taccgcctat ctcttttttt tccacataaa tatattggct ggatttttga
    16621 tctgcatttt gatcatttgt tgtcttattt attttcattt atttctcttg ggaaatagta
    16681 atatttatta cttcaataca ttcgctctca actattccct gatacttggc aaaaaatttc
    16741 ggaatgcttg atctccaaac taattaccga tgagaaaaca ctgaagtgcc attaacttct
    16801 tttatctggt ggatttgaaa ttttaaaaaa ccgagggcta tctgatatag cgagactttt
    16861 tcgcacctta gagattgatt tcgtatacca gggttgatac cactcatttt cagcattcgt
    16921 tggcccgcag taacttgctt ttaccgagct gaaaaactgt tttttgttat gtattaaatt
    16981 gcaaatcctt ttcaggtttt gcatttcttc cggagaaata aaaactggtg cagtatctgg
    17041 caaatcaatt ggcttgagaa tccacaattt tggaaactat ttttttcata aatagaaatt
    17101 ttttataagg tgagaccaac ttccgtatgt ccacagtcag gataaatata ttttacgcaa
    17161 ccaaactgga cacatttttg tgttagatca ctgtttttcg taccataaac taataaatta
    17221 attcgtgcct ttttcttgaa tcaaccatag ggcaggaaga gtcaacaaag ccatcaccat
    17281 caaataaata gaaaatgcaa aatggatgaa agcataactt aatagcaaaa taatgagtgg
    17341 tgctgtgcct ccaaataggg aattagacaa gttaaaaaat aaagtaaagc cactggcgcg
    17401 ctcttcctgc ggtaattgtt caaggctggc ggtaaatgcg cttccaataa aaaaagagat
    17461 aatgacagcc aaaacactgc aggcgacagt aatttgtaat aaagtacctg tattgatcaa
    17521 ataatttatt accaaaacgg tacataaagt tgcaattaaa ctgactgcca aaggaaagcg
    17581 cctgcctaaa atatcagagg tgaaacctga taatacgaga cccatgctat aacaagtcat
    17641 ccctatgata atgacatcca tcgctgtagt taatggcaac tcctgctgtt gcaggtatga
    17701 aggcatataa acaaaaaatg tataaaagct actgttgcaa taaattgcca aaagaaaaat
    17761 agcagcaatt gcttttttcc gataacgcca ggcattcagt atgggatgag attgtctttg
    17821 aacaattact tttgcaaaat cggttaattc tcttgtttga cttcgcaaat aaagtccgat
    17881 aaaacctacg aatattccta ttaaaaatgg gaagcgccag agaaaatcgg gagactgact
    17941 ggaaaaaaat atcagggtta ttttgcttac tattattgca agcaacaatc cacaaataga
    18001 acctaaagtg atgaaactga ctgctaaacc tttatgacgc gtgttcacat tttcataaat
    18061 gtaaatactg gctagtgtca tttcccctgc tacagaaacc ccttgagtta atcgacatag
    18121 cgtgagtaat atcggagcga gaatgcccgc ctcttgatag gtaggtaata aaccaataaa
    18181 acttgatgaa aaagtcatga ccataagagt catagtcaat gtttttttgc ggccaatttt
    18241 gtcacccaga ttccctaacc aataagcaga gaatggccga acaagaaaac ctgcggcaaa
    18301 aataccaaat gttgcgatga gagcagtgtg tttatcagtg gcaggaaaaa aaagaggcgc
    18361 caaaataggc gccataaacc cataaatggc atagtcgtac atttcaaaca aggtgccaaa
    18421 agaggctgcc gcaataattt ttttatccat gttcaagctg ttgcttttat ttcacaggta
    18481 gtaatacagc ttactttaac tctattgatt tctgagcttg attctctgca tccacttcat
    18541 ccttgactgg tttaggacga ccaaaaaaac cggttatact aggccttgat ggtaacacag
    18601 agacaatcaa ttgtcccaat gaaactgttt cggactcaag aggacttggt aattcctcaa
    18661 gcgagagttt ttgcgggtga ttggcgatat agtcattact gtcttcgaga tatttacaaa
    18721 taaatgattc ataaaccccc agacctgtat catactttag caaatacaaa tcggccaaat
    18781 gcgtgtccaa ttgaccgtca ctgtttctgc catatttcgt ctcgagaaat cttattcctt
    18841 gttcgcttaa tgaggcccaa tgccactgtt tatagatatc tcttttaata cgatactctt
    18901 caggacaggg atcaacaaaa tgttcaagtt gagaattgga ttcaacgctt tctgcaagcg
    18961 ttgcatgccc tgcccctaag gtaagatcac ctgtatgatt gatatgaatt tggtaagggg
    19021 tatccaggcc atagttatcc cctgggcgaa tatcccaacc agtatagcga agcagagggc
    19081 ggactgtata acgcaaagta gagaacgcac ccagatttcc ttcgacagca gcttgaaaag
    19141 tggaaaaggt attattcaga atgcatctta ctgttacatt tgattgcagt ttgaattgat
    19201 cactgacttt ttttgccacc gcagcaccaa aggaatcccc ttgaaaaagg atatcatcaa
    19261 ttgatatgcc tttttcaagt aaggtattgg ccagcgctat tccagtgtta accatatcat
    19321 tgacttctaa cacttcaccg cccagttttc tcatgcccgg gtattcaaaa gcgtaataat
    19381 gcgctcccgt tatttgaaca gcttttgcta tatcaatcag gcaatcttga tattgtgttc
    19441 ctattccagt gaaataaatc acatgttttc cctttccaac agctttgacc ttctcattta
    19501 gtaaggagct tgattgattg gaattattct gaaaccctat cacctcggtc atacaggttt
    19561 tttggttggc taaagtcacc tcgatttcaa aacgatgaaa gtctataccg gataattgtt
    19621 catgataggg agaatcctct tgacgtattg cctctacaaa acaggcaaaa cgttcttcaa
    19681 gtgacatctc tgaattaccg aaaccatttt tgacatacca gtcctctcta ttgggaggaa
    19741 acgttcttaa agctatttgt ctttgcaacc agatttgtat acttgaattc ggcatagcac
    19801 cccccttttt tttgatcaat aagtgtgcat acaatttgct aatgagtcaa catttctttg
    19861 cgcatcttta ttagagataa ttcttatttt cagcacagca ccgatttgca tccgattgca
    19921 acaaaacaaa ctcactttaa ccctgcgctt ataagaaatt tgtatagata acatacaaac
    19981 aattgatttt ataaatgaac agtcggttga tattattccc tatttgatta aaacaaaatc
    20041 tatatatttg ggagattttc accatgtttt acgaattaat attaaccaga accagcaacc
    20101 tgatacaaga atttatctct atccctcatg gcgttatttc tcttgacttg agtttgaatg
    20161 aattgggcaa tataagtaat gctgaattaa ttcaggcctt taagtatata cctgacagcg
    20221 ttatatcgtt ggatttgact aacaatcact tatgcgacaa aagtggtgca gaattggcac
    20281 aactcctggc cgccatccct gccagggtaa cttctctcaa tttaagctgc aatgatctcc
    20341 acaagaaaag tggtgctgag ttagcacaag cctttgctgc tatcccagcc agtgtaactt
    20401 cgcttaattt gcattgtaat tacctgggca ataaccgtgg ggtggagtta gcacaggcct
    20461 ttgcggccat ccccgaaaat gtgacctcac tggatttaag tatgaattat ttcgatctgg
    20521 aaagtagtgc tgacttatca caaattttta ccagtatccc tcctcacgtg gcgtcactta
    20581 atttgagctt taactctttg catgaagtac cctttgaaaa gctagtactc tttaaggatt
    20641 cgctaaagca tgtgcaaact gtttatttga gcttttatag tgtcaaagaa atgtccaaag
    20701 aacaacgaag agcactgggc gctgcatttc ccaatgcgca aaaaatcatt ttgattgatg
    20761 attatggcca tgaaattcaa ccgtctataa taatttctaa cctgattagg gaactttctg
    20821 gaaaggcaga tgctccatcc ttgttaaatc aatgtattct ttttacccaa agacatcaaa
    20881 aagacagtga taacaagatc atccctaagg aattagaaga gagcattcga acttttaact
    20941 ctcactgatt gataatcttg agcaaccgtc gttcgctctt gtcaatgaat taaaaaatac
    21001 caaaaacttg tttgactctt ccgagtcaaa caagaaatgg aataatcggc aaagaaaaag
    21061 aactcttgtt aatctttgaa agttgtcggg gaattaagca ttttggatat ttctccaaga
    21121 tgcatttctt cttcttgaat catttgtctt gcaaactctt caactaacac cgacttattt
    21181 tctgaatatt gtaatagttg ataatacaaa tccagacttt gcctttcatg ctcaagcgac
    21241 tcttctaaaa tattgctgat attatgctca tgagtctcta ataacttacc gattttaagt
    21301 gtaggatgat aggaaaaatg ggtaataagc tcaccgatta gatgagcgtg gttcaaactt
    21361 tcttgagcct gatcgcgcat ccatttgcag atcgggatgc gattaaatcc aaagaccata
    21421 aaggagtaat gagtatagcg cacaacaccc gccagctcga tttccaataa tttatttaga
    21481 gtttttatga tggcatcatc aatgtcttga gtttcagatc ttctatgttt cataggatta
    21541 cctgtcattt tacttatcta agtatagaat aatttcaaga aaatctcgaa gcgttatcag
    21601 ttattgagaa ttgcctctca tatctaacat aagctgtgat acaaagatga catctggcag
    21661 tcttggataa ataaaaaact ggctacacaa aattattatt caattttgcc aatatattgc
    21721 cacttctatg atttaagggg gcttgataga agaaaattat gttcttttta tttactactt
    21781 aaggattaat agaaaacatg gggcataacc tgatcttaat gatctatatt aacgacaaaa
    21841 acataaaatt tataaatatt gaaaaaaaca tttgcattta actcaagatt tgcattaatc
    21901 tgaatgagac tcacaaggaa aaggagatca ctatgagtaa caaagcgaaa aacactcacc
    21961 tcaaaaatgg agatagtcaa accaaacaag cttataccaa tcaggcttca ccactttttt
    22021 ttagtcaaaa gcaaataacc ccctcatcgg gaacatcgga aacaaaaaaa acacaggaag
    22081 gaaaacaacc cacgcaagaa atcagccttg agacaaccac agacatttta ttagccgcta
    22141 ttttgcaaga attaaaaacg caaaatatga ttgaactcat gaaactcaaa gctcaacagg
    22201 agcaggagca agaggaattg caagccattg agacaatgaa agagaaagat gacgcccggt
    22261 ttaatgaaat ccggcaaact atgtatgcct gatggacagg acatccttct taaccaatta
    22321 acagatcact gttaattggt ttttccacca aactgagtaa atgttacctc aaaagaaaat
    22381 actgtaattt tattagacct cttgcataat ctgtttattt tatttacgca tcacatagta
    22441 ggtattcata atgtcatgtt ccaggtaatg cttttcaatt tttccaaaac cagcttcaac
    22501 cagcattttt tctgcaagct cctctcccca ggccgcgcca agacctaagc cattttgtga
    22561 gagagataca gtcatacaat gcatacagga gattgtgtaa ataagcgccc ccagaggatg
    22621 atgaacattc cctgccacat cactggaagc tttgatatcc tgcatcagga aaatacctcg
    22681 aggcttcaat gccttgaata cgttgctcag tacctgcgct ggcttagcct ggtcatgaat
    22741 cacgtcaaag ctggtaataa gatcatattt ctcttgctca tcccagaccg acaaatccat
    22801 tttttcaaaa tggatatttt gtattttatt ttccttggct gaatgtacag catgagcaat
    22861 cgtttcatca cacagatcat aaccggtgaa ttggctttga ggataggttt tagccattaa
    22921 tatcaaggca cgcccataac cacaaccaat atcgagtatc tgaattccct ggttcagttt
    22981 gtcaaccaag ccgtgaacca taggtaaaat tttgtcagtc aaggaagata aaatcgtttg
    23041 tccactctct tccgccatga cttcatgaaa acgatgataa gcctcataag gaacaccgcc
    23101 gccgtgttta aaagcatcca ctacgccatt ttccacatag gccaaaactg gaataaactg
    23161 cgctaccact gccatattct gtggcgaaga tttcctcgta agaaaagaag catgttcagc
    23221 gggtaattga taggcttttg catgcgcatc ataatccaca atgcctccca ccacgagagc
    23281 attgagccat tccctgacat aacgttcatt acaacctgcc gcctcggcta tcacttcact
    23341 gcttgcaaaa tcaagctcgt ccatgacatc aaacaagccg gtacgatgcc caatggatat
    23401 catcaaagtt aatgcggcat cattgagaac agacaaaaga cgctcagcaa acgcatcagc
    23461 tcgggcttga tcaaaacgtc ctgttttcat cttgcgctcc ccaaaaaggt aatcttcaga
    23521 atcccataga gaaaataatg tatctaacgt agtagataag gattataaaa gaagcagtcg
    23581 ctatccagag gtcgtatggg ctgaggagat gcgtacgcat cgtctcgaag ccttggcaag
    23641 aaacattaaa ttccatacaa ggcttcgaga cggcataaat gcctcctcag cccgaacgat
    23701 tcaagacaac gacctgcctt atccataact cacgttactt catattatag actatgagcc
    23761 attccatgtt atgagcatca atttgccagg gatccattta aactttcgtg cataaaagcg
    23821 tcttcttata tcgccttatt cttatcgtct aatattcgat tatttttctt caaaattcat
    23881 caaagcattt tcatgaacca gctcatgatg atgttctgtt aatctcccct catagacaat
    23941 aggggattcc cactcatcgc cggcaaatcg atattcttct gtagaaaaat gttcaaatgc
    24001 atagggtatc ccttggccaa ttaaaggaga tggtttatca ataacatgaa aatgcaaatg
    24061 cggtgcgtca gaattgcccg aattacccaa ttttgcaatc aattgattct tacgaaccaa
    24121 ctccccctta gtgaccttaa tactatgggg aattaagtgg gcatagagag catattggtg
    24181 ttttcctatt ttaataatca cgtgattgcc agccatatta ctcagcgtaa caggataatc
    24241 acgtccaggc ggagtattat caggaacacc atcgtaaact gcgactacct caccatccgc
    24301 aaccgagtaa acattggccc cataactgta atagttttca tttttgttgg gattttcttt
    24361 atagacatga ccatcaactc caaactgcat gaaatcaatg gcgtatcgtt cggggtaata
    24421 cacaatgcca tgggctgcaa cccttgcgcg gcggtgtatg gaatgattgg atgggccatt
    24481 aattgccatc caatgctttc catccaaagg gttggcaatc accaggggct tagtaatttt
    24541 tacataagcg ggagcagcca gaaaagaata cctgacaccc tcttcggata ttttggtttt
    24601 cgatgaataa agatgaaacc gatgcattat tttttcaggg atttcatcaa gactatcgaa
    24661 tttcaccata aaaaataaca aaccacgctc acccggtctt aatgtcaagt cagatgttaa
    24721 atgattagaa tgaccactaa aagaatatat catctttcgc agctcttcct ggttatagga
    24781 aaaaagaact ttataaggtt ttctgccatt taatacttca acgttttcta aagagaagag
    24841 gtaatcacct acattggcaa gaaaaacctc atacgtgaga taaaattgtc cttcaatagt
    24901 cacgggtttt ggagaataaa acgtttcggc ttctaactga aaataaacca atttatcctg
    24961 attggattcc aatccagagg gaaaagcatg catgtctcgt gcgagaatca aaccacttac
    25021 aagaaataaa aaaattgtga cacgaaatga ttttatcatt taaaaccctt ggtaaattgc
    25081 caaaacaacg ttctattttc aataggctca taatcaattc cctgtaatca atgacaaggg
    25141 ttttatcatg atgagatgat tgggatcctg gctgaaacct gtttgctgaa agcccgcttt
    25201 ttcgtagcaa cgaatggctt gttgattgtt aacatcgggg tcaacaacaa cagctttgaa
    25261 acgggtaaga aacttatcaa taaagcgatt aatgagttcc acccctaatc ctctacctcg
    25321 tccctcgctt tcagcaataa acaaatcgat tccagctaaa tccctgggat tatatttttc
    25381 aaagagaaga ctctcataac catcaattcc atcgggtaaa gaatctgtga tcaaataaaa
    25441 ctggacgaac ccgaaaggta agtcatcttt atatacaatg aaacttggaa catgctccat
    25501 acccgcaatt cttggttcat atttttctgt gatttcatcc agagaccagc ttttgtctct
    25561 cgcataccaa tgattaatga ttggctcctg aaaccattga tacagttgat ttaaatcctt
    25621 gatgcccaga ggttgaaatt taaaaacact catgactagg ctctttgaat cccttatcaa
    25681 tgccatgacg actcacatcc gaggcatgcc aaattgactt ccataggtca ggtttgtggg
    25741 taatcatgat tcactatact gtagtcaaaa actgagtggg tattctacca cagttttgat
    25801 cgcctgggtt tgcttgtgat tgacatagaa gatcatctca tcagtgcagg ttgagcaaac
    25861 tgcccaaccg ctaatctaat tcaataacgc tggatcaaat ggttgcccat tcactgtaaa
    25921 tttggtgttt tccagagtaa aagaggcctg gtagtaatcg ccttcaactt ttataatgcc
    25981 tttactcacc aatttttgca agagttcttc cgtttgttta tgggcttcgg catccgcatc
    26041 aaccagcggc tctgtaacct gagattgaga catatctgct gctggtgtag ctgtttttga
    26101 tttgattgat tcaaatagta gctgttctac cagtttaatt ggcgctttaa actgaccatt
    26161 gcctttcaaa ttttgcaaga gatcttcagg attgctgaat gaactcttgg ttagagccag
    26221 gttcaacgaa ctggacaaat gcccctgtgg cgtatccagc tcttgattca gattgagtgt
    26281 cgcgcctttg gaaagcaggt taaacatttc agccaggatc gcgatttgat tggaaggata
    26341 gtcatagttc aagtgattga gcttggccat cgcttgagga tccagatttt taacctctat
    26401 ttttaactgg ccaggaccat agctgttatt gttcataaca aacttattca gggataattg
    26461 caaattaaaa tccagtaaat tgctgttgac tccagcccat gcatccatac ccaaattttg
    26521 taaatcaaac actggctgtg agttagtgat gatagacaaa gaagacagca tgatagatga
    26581 atcaccaagc cataaatcgt ccatgtagcg attcaatgca aaattgacct tgactttccc
    26641 aatccttaca tcggtttctt gaacctgagt actcatgccg tacatagtca catggccaga
    26701 ggccttatca atcctgggat tgactttgag gcttgcactt acgccttgcc agacaaaaac
    26761 aaatttgcct tgatcaccgt tattcgtacc ctgcatatta atcgagggca aactataact
    26821 gacagcggtt tgatttaagt aattaatcaa agctccataa tgagtttccg gtttcgtggt
    26881 gacccatgct agcccaaaac gcaagccatt attggtcatg atgaaagggc catggcgcac
    26941 aattacagga aaatccatgc tcaaatgaag tgctggctga attttaatat taccctgagc
    27001 gtctttattt tcctgggctg gcaaatcaac aagtaaattc aaaacggcag tcgagcagaa
    27061 ccatccactt tgattatctt ttaattgcca agtcaagaag ggactcttgg gtatgttatc
    27121 gacgtttttt ttcaccgtgc ttaaggccat catgccaatg atagggtatg agcataagac
    27181 gatgattact aacagcagca aactgataat ccattttctc atggctgtcc aatattaatc
    27241 cttggtaatt aatatttaga ctagaaaagg ctgcttgtaa aatagacagg acttacgaa
//
LOCUS       DAXLDT010000031        26710 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXLDT010000031 DAXLDT010000000
VERSION     DAXLDT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26710
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.031"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1985)
                     /locus_tag="ACF2SP_001017"
     CDS             complement(<1..1985)
                     /locus_tag="ACF2SP_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358817.1"
                     /translation="MTKNTSKSVFGGQYVYYYDFPTEVEVDFSLLPPEERQPILIAQD
                     DSYTIETNRFLDYVKKIKVECDDKMAWVISAVRDEFGSLEDVISIRQLCEYCNTSDYW
                     KKDGEWPQDVNDEQLSSFNMQVLRNWLFQKTVRFFYAHNPDALPSITEYKLQLQANLR
                     QFKQEILALNALQLMESETDTYLATFWESVTSNKISDRFNVFNISSVKEIFKEYELIP
                     DGMPLCIHPDNFEYWQQIHRKAIDELAVIINSSIMDELKKKSAELCYEIPFEQLYATY
                     WPSSKRFDDNTLPLRSLSEINEFMEKISLIGQQRIIMIEQKRKEWKGSPAGFDVAVER
                     AQSVLSSQKGNNQEFIQVLSEELKKIAAEDNVLESLSLVNTNPTQVVKQILNQEGVSN
                     VGKLAPVIVRDIQHVIEHMGDKLTLADHSKNVETLDQFVVNVRSACNREALIDLYDRL
                     EKSTSKEMAEKIISNWDIVGTDIRDSARMMTESDKTRIIAYAEGKMTKERYQQEIAKL
                     SVKLNQGEITAQEEVVIKMFPKLKQALMRLPKEYFEDFSLVLKRAFVNQQITHPEKFL
                     QNPHEMAVFSAFGLDPSKIKANKELVQQKIKHVIQNAVLNGQAENEIPTLKELQHQIE
                     LMALKEKIIAAGGESSLTPAIHTLTKARLEIITLATN"
     gene            complement(2558..4432)
                     /locus_tag="ACF2SP_001018"
     CDS             complement(2558..4432)
                     /locus_tag="ACF2SP_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFD2358818.1"
                     /translation="MLQKLNEHIQGVVAWLVIILIAITFTLFGVDYYFQSRQISDAKV
                     IVNDKPITMQAFETNYRRTRAQQDLPQMTAADEKNLQNQVLNQMITNEVSIQAARKYG
                     FEVSPEQANAAIVQIPQFQEDGHFSAQRYQQALSGALFTPETFQNEVRQGMLLNQQRF
                     AFMGTSFALSDEIKRFVRLYMQTRDYDYLTVPSDRFEQQAKISEDDIKSYYNQHHKKF
                     MTSEQVTLDYVLLSMHDIKSQIKISPDQIKSYYEENKSNYLTPAQWRVAHILFAVPEN
                     ATKEEQDSIKQKADEVYSDLKKHPEQFDKYVASKSDDKLSITNKGILPWITGGQNEYG
                     RALSNLTRPGQISIPVQTKYGYEIFKLIAYKPVTTKSLQEVENIMKDQLTSDMAQAKY
                     AQVLEQLTDLSYQTPDSLDPVLDSLQLKIQHTEPFSRDGGKQPITKNKQVIHAAFSTD
                     VLELGNNSEPIQIDNDSVIVLRVKKHIPSREQTLDEVRDQIKKILAKQYGDAKAKEIG
                     SSLLHPVEDKQQQELIANNQFEWHSVENASRDSDKANSLINDLAFNLLRPESRDGVIL
                     DNGDYVVVKLKRINDGKLSSLDQEQRDSLIQQIEASYGMMDYDLYVNNLLHHANIVRK
                     "
     gene            complement(4524..4600)
                     /locus_tag="ACF2SP_001019"
     tRNA            complement(4524..4600)
                     /locus_tag="ACF2SP_001019"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4564..4566),aa:Asp,seq:gtc)
     gene            complement(4607..4682)
                     /locus_tag="ACF2SP_001020"
     tRNA            complement(4607..4682)
                     /locus_tag="ACF2SP_001020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4647..4649),aa:Val,seq:tac)
     gene            complement(4690..4968)
                     /locus_tag="ACF2SP_001021"
     CDS             complement(4690..4968)
                     /locus_tag="ACF2SP_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFD2358819.1"
                     /translation="MNKSELVDAIASGSGLTKADASRVLETFMATVTDVLKKGDQVVI
                     PGFGSFSTGNRSARTGRNPQTGKTIQIKASRVAKFKAGKNLKEAVQQA"
     gene            complement(5097..7511)
                     /gene="lon"
                     /locus_tag="ACF2SP_001022"
     CDS             complement(5097..7511)
                     /gene="lon"
                     /locus_tag="ACF2SP_001022"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFD2358820.1"
                     /translation="MKSSALPVLPLRDVVVYPHMVIPLFVGRGKSIKALEAAMIDNKQ
                     IFLVAQRKSAHDDPGPEDIYQVGTVSSVLQLLKLPDGTVKVLVEGEQRARVKEYTQDK
                     GYLEATLEYIEEVGSTIQEQEIGILMRSLMSQFEQYIKLNKKIPPEVLSPLAGIEEPG
                     RLADTIAAHLTLKVDDKQELLETMDVGTRLEKLMAAIENEIDLLHVEKRVRGRVKRQM
                     EKSQREYYLNEQIKAIQKELGEMGEEGNEIEQLENSIKKAGMPKEAKEKSLAELHKLK
                     MMSPMSAEATVIRNYLDWMLMVPWKKRTKIQFDLLKAEKLLDKEHHGLEQVKERIIEY
                     LAVQQRVKRLKGPILCLVGPPGVGKTSLGQSIANATGRTFIRIALGGVRDEAEIRGHR
                     RTYIGSMPGKIIQKLCKAGVKNPLIMLDEVDKMAMDFRGDPASALLEVLDPEQNHTFS
                     DHYLEVDYDLSDVMFIATANSLEIPAPLLDRMEVIRLPGYTEDEKVSIAEKYLVPKQM
                     LLNGLTNEEIHISEGAIRDIIRYYTREAGVRNLERDIASICRKVVKEILSSKKGKKGK
                     TISITVSNITKYLGVKKYRYGLAEQYDQIGQVTGLAWTSVGGELLTIEASIMPGKGKV
                     THTGQLGEVMQESIHAAMTVVRSRAKAFGLPDDFYEKHDFHVHVPEGATPKDGPSAGI
                     GMCTVLVSIVTQIPVKADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKHVIIPEEN
                     VKDLEEIPDNVLRKLTIHPVNTIEQVLELALQRNPWVEEPTNIQPGTILNKRSKKIKN
                     NDLHAH"
     gene            complement(7781..9055)
                     /gene="clpX"
                     /locus_tag="ACF2SP_001023"
     CDS             complement(7781..9055)
                     /gene="clpX"
                     /locus_tag="ACF2SP_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFD2358821.1"
                     /translation="MSKSGNGNGDKVLYCSFCGKSQHEVKKLIAGPSVFVCDECVELC
                     NDIIREETHETHEETEARLPTPKEISNFLDEYVIGQQHAKKVLSVAVYNHYKRLQHKS
                     EDGVELGKSNILLIGPTGSGKTLLAQTLARILNVPFAMADATTLTEAGYVGEDVENII
                     QKLLQKCDYDVDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVA
                     SVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRERSDKSSIGFSAQLKSKKS
                     SNDEASKVLGQLESDDLIKYGLIPEFVGRLPVVTTLQELDEAALIDILTRPKNALTKQ
                     FQSLFKMEGSELEFRDEALIAIAKKALERKMGARGLRSILENILLDTMYDLPSLEGVN
                     KIVIDESVVNGLSKPILIYEQDEKKSASGSKD"
     gene            complement(9196..9840)
                     /gene="clpP"
                     /locus_tag="ACF2SP_001024"
     CDS             complement(9196..9840)
                     /gene="clpP"
                     /locus_tag="ACF2SP_001024"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFD2358822.1"
                     /translation="MPGYSDNIIRNASGLIPMVIEQTSRGERSYDIYSRLLKERIIFL
                     LGEVEDHMANLVVAQLLFLESENPEKDISLYINSPGGVVTAGLAIYDTMQFIKPDVST
                     LCIGQAASAAALLLCAGAEGKRFCLPNSRVMIHQPLGGYRGQATDIEIHARETLAVRE
                     RLNNIMAKHTKKTPDQIMRDTERDNFMSATQAMEYGLIDKVLYDRQVAGHSTDL"
     gene            complement(9843..11174)
                     /gene="tig"
                     /locus_tag="ACF2SP_001025"
     CDS             complement(9843..11174)
                     /gene="tig"
                     /locus_tag="ACF2SP_001025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFD2358823.1"
                     /translation="MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGF
                     RPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKD
                     FKYTAVFEVMPVFEIVELNQAPVELIRSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
                     GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFP
                     EDYGHKDLAGKEATFKITIKKIMEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMAR
                     ELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIP
                     DFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWY
                     QSSKEHMAEVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGE"
     gene            complement(11204..11288)
                     /locus_tag="ACF2SP_001026"
     tRNA            complement(11204..11288)
                     /locus_tag="ACF2SP_001026"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11252..11254),aa:Leu,seq:tag)
     gene            complement(11379..11454)
                     /locus_tag="ACF2SP_001027"
     tRNA            complement(11379..11454)
                     /locus_tag="ACF2SP_001027"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11419..11421),aa:His,seq:gtg)
     gene            complement(11615..11691)
                     /locus_tag="ACF2SP_001028"
     tRNA            complement(11615..11691)
                     /locus_tag="ACF2SP_001028"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11655..11657),aa:Arg,seq:tct)
     gene            complement(11697..11773)
                     /locus_tag="ACF2SP_001029"
     tRNA            complement(11697..11773)
                     /locus_tag="ACF2SP_001029"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11737..11739),aa:Pro,seq:tgg)
     gene            12045..13271
                     /locus_tag="ACF2SP_001030"
     CDS             12045..13271
                     /locus_tag="ACF2SP_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2358824.1"
                     /translation="MIMKTKLLPLFDNLNHIHKNHENVILPDARFQNDFTYCLSFLKS
                     YIGSEGTFNSYRRETERLLQWSWLVKNSTIDSLRRDDIEQYIHFCQNPPKTWISLKKV
                     PRFIEKNGKRIPNEEWRPFVVTLSKVSRKNGHLASIDQFKLSQGAVQEIFAILSTLFN
                     FLIAEEYLISNPVALIRQKSKFIRKRQHNAPVRRLSLVQWEAVLRAAESLAEDSPEKH
                     ERTRFILSLLFGMYLRISELAASERWVPSMNDFAKDSNGHWWFTTVGKGNKERQIAVS
                     DAMLESLTRWRLFLGLTPLPSPADNSPLLPKLRGHGPMSDTAPIRRIVQHCFDLAEEQ
                     LRLNGLSEEADSLSAATVHWLRHTGISEDVKIRPREHVRDDAGHSSSATTDRYIDIEK
                     QARYQSARKKIIQPSE"
     gene            13394..15244
                     /locus_tag="ACF2SP_001031"
     CDS             13394..15244
                     /locus_tag="ACF2SP_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFD2358825.1"
                     /translation="MLTLRQITLSRGDKTLLDKVNVSLYEKQKIGLIGHNGCGKSTLF
                     DFLLGQLSPDTGEYLINPQLSISHLSQHIPDSNEKALDFVLAGDQQYIKLLKRLSEAE
                     KTGDDAEVLQCHEELSHTGGYSKPAQAAVIMSGLGFSGNQQQEPVNSFSGGWRMRLNL
                     ARCLMKPADLLLLDEPTNHLDMEAIFWLERYLKQSPSTIILISHDREFLDAFVTHILH
                     IENHNLTLYSGNYSSFEKIHAQQLALQQAMHEKQQTKIKHMMTFVTRFKAKATKAKQA
                     QSRMKAIEKMEIIAAAQADSPFSFDFFPCPRAGNPLIQCNQVDAGYINHPPILKKIKL
                     SLNPGDRIALLGPNGEGKSTLIKTLTADLPVLSGDIHRSPHLQIGYYAQHQLEQLDCK
                     LSPVETLQTLSPEVREQTIRDFLGGFNFRGDMAVQSIQHFSGGEKARLALARLVWLKP
                     NLLLLDEPTNHLDLGMRSAIELALQTYEGALILISHDRHLLKSSVDNFYLIYQGKVQP
                     FEGDLDDYYLWLQTRNSEKESKLATSPNEYKERKSYQNKLKKLEQLIEHYQNQLRLLD
                     KQLADVSLYEDTKQKSKLDNLLMERDIAHSALIKTEEEWLEICHALESLE"
     gene            complement(15272..15946)
                     /gene="rnc"
                     /locus_tag="ACF2SP_001032"
     CDS             complement(15272..15946)
                     /gene="rnc"
                     /locus_tag="ACF2SP_001032"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFD2358826.1"
                     /translation="MKINLERLCRRLNYHFNNIAYLKQALTHCSAGSDNYERFEFLGD
                     SILSFVIANELFNRFPLHSEGQLSRLRSFLVKGEMLAEIAREIGLGDYLFLGQGELRS
                     GGFRRTSILADALEAILAAIYLDGGMTAAKQIILMLYSSRLDDPDLNHCLKDAKTQLQ
                     EFLQASKFALPEYVLTKIEGDEHAQIFHVTCTIEGVSQVAYGTGPNRRKAEQLAAKAM
                     LEQLQG"
     gene            complement(15936..16328)
                     /locus_tag="ACF2SP_001033"
     CDS             complement(15936..16328)
                     /locus_tag="ACF2SP_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HFD2358827.1"
                     /translation="MRKQQGMTLIGMLFTAVVVIMAGILVMRAAPVYIQHYSILHSIK
                     ALNSTPVSSLSGDPLTDVTTLRESVTKRLDINGVEDLKPGELVITPSGPNKFKIKLKY
                     QVIKPLIYNMSLLFNFEDNFEVITGSEN"
     gene            complement(16340..17095)
                     /gene="lepB"
                     /locus_tag="ACF2SP_001034"
     CDS             complement(16340..17095)
                     /gene="lepB"
                     /locus_tag="ACF2SP_001034"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFD2358828.1"
                     /translation="MNFALILVILSAVTGLIALLDWLIWSKKRLPEQKPGRIIEYSRS
                     FFPVFFIVLLLRSFLIEPFRIPSGSLEPTLLVGDFVAVNKFSYGLRLPVWEKKIVPIA
                     NPKTGEIAVFRWPPEPSYDYIKRVIGVPGDKISYHDKKLTINGKEVKQTFVEYTTDES
                     SGKAVTKYKEDLNGVVHDIFVRPDAPAVDFDIVVPEGNYFMMGDNRDDSADSRFWGFV
                     PDSYLRGKAFLVWMSWNGKTDNVRWSRIGKLIH"
     gene            complement(17203..19035)
                     /gene="lepA"
                     /locus_tag="ACF2SP_001035"
     CDS             complement(17203..19035)
                     /gene="lepA"
                     /locus_tag="ACF2SP_001035"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFD2358829.1"
                     /translation="MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLT
                     EREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSR
                     SLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
                     DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGV
                     VSLVRIVNGAIRKGDKMRVMSTGRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKE
                     IQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDA
                     SLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGE
                     TLLIDNPSHLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRH
                     VSVVYDIPMSEVVSDFFDRLKSVSRGYASLDYNFQRFQIADLVKMDILINSERVDALA
                     VIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKNVTAKCY
                     GGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK"
     gene            19230..20246
                     /gene="mltB"
                     /locus_tag="ACF2SP_001036"
     CDS             19230..20246
                     /gene="mltB"
                     /locus_tag="ACF2SP_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFD2358830.1"
                     /translation="MRQIIWLGFISLFLLSFTTYSDTTFTKRKDVQSFINMMVTNYHF
                     NRNQLTTVMNQVKLQPQVIESMEKPYEKKNWDVYRELFLTQKRLKSGLQFWAENQKTL
                     EKIQRRYGVSPEIIIAILGVETMYGERQGEYRVLDTLATLAFNYPKRSAFFTKELKEF
                     FLLCREQHVSPTQYKGSYAGAIGKPQFMPSSYRYYAVDFNNNGRRDLVNDNGDSIASI
                     ANYLHKHGWNLNQGIAQPAKISGWRYKQLRMNPKTANYRYSQLISYGVKPITASQNHP
                     PRAGVIELVTRKGKEYWIAYPNFFVITRYNSSPQYALAVYLLSQQLKQHWYTSATKRH
                     RAYV"
     gene            20321..21460
                     /gene="lspL"
                     /locus_tag="ACF2SP_001037"
                     /gene_synonym="gspL"
     CDS             20321..21460
                     /gene="lspL"
                     /locus_tag="ACF2SP_001037"
                     /gene_synonym="gspL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL family type II secretion system protein
                     LspL"
                     /protein_id="HFD2358831.1"
                     /translation="MDKCFIFSKHLDDNGCVCLKISDLGELIAPPEFRNFLQIKDLQK
                     ESSTIIVETCTRAILLGLALPWLPERKARIAIPYALEDKVTQPVEELHFAFDKFYYQN
                     NQYLVVVIDKQRMRKLIQVFDEHGIEFDAVTLDWFALASQELIISETELLINNEDFKG
                     VLSGDLAQTYLKNHPHNPAHLFQDSKIPVTSPASQNEEHSYTWISKRLIKSRPLNLCQ
                     GEIQHGKTTDWVKKGYLLVGGLCGFWLISILFVNWLSLHWLNRQIDEIDRQTAVIYRE
                     FFPDAKQVISPKFRISQLLGGSTAENQTRFWFILNQLSKAIKDSGITVEQLRYQNKTI
                     SITLVSSDFESLQKIENKLKQSQLKVKQTQASTKDQQVVATLELT"
     gene            21457..21927
                     /gene="lspM"
                     /locus_tag="ACF2SP_001038"
                     /gene_synonym="gspM"
     CDS             21457..21927
                     /gene="lspM"
                     /locus_tag="ACF2SP_001038"
                     /gene_synonym="gspM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     LspM"
                     /protein_id="HFD2358832.1"
                     /translation="MKTYLNTLNDREKWMVIVAGVSLFIYGYYLLLYAPLSNQVNQKS
                     TQLIEKTETLEWMKQVRMQKRSAKRKESVDNSQLLTMLASQLKNNKTLKFPYQLQQTG
                     SGDVQLTFDEVPFQNFIQWLAKINEVYSINIKQFDVEKTPTPGVTRLMIILSTS"
     gene            complement(22031..22564)
                     /locus_tag="ACF2SP_001039"
     CDS             complement(22031..22564)
                     /locus_tag="ACF2SP_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFD2358833.1"
                     /translation="MMGISIRYIILGISVMNISSCMVYEDTYTTRYQIYTYDYGQLYP
                     QADYHARDYNYGNQSGQGVVVPDSYHVGEYHSPVSFKDRDRNWVNSQNPQGYTIQIAD
                     DEKASYVAQKLYKAPKNDRMAQVKYYQNGKTHYKGLYGTYDSQEAAQKALESLPPEIK
                     QGAGIKDWGSIQGNLNE"
     gene            22839..24164
                     /locus_tag="ACF2SP_001040"
     CDS             22839..24164
                     /locus_tag="ACF2SP_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage deoxyguanosine triphosphatase"
                     /protein_id="HFD2358834.1"
                     /translation="MWTHRRSGQTNQRGSQDHRDPYERDRTRVIHCPAFRRLQRKTQI
                     LGTDEGDFHRTRLTHSLEVDSIGRSIVRNLMTNQEHQLLLSSLLPNDDLISVICLLHD
                     IGHPPFGHGGEVALNYMMRHYGGFEGNGQTLRLLTKVENSYGGFGLDLTRRALLGILK
                     YPVKRANVVAPQQPPIHESIHKTIKINDWLPPKAYFDCEQPEVDWLLSPLSDNDRELF
                     QSLSVKPNGTQAGKAAYHNFDCSIMDTADDIAYGVHDLEDAIHLRLINRSHLDTPEFR
                     QLISETTLSLHRERLLNSLFSPEICLRKQAIGEMVNYFITSTQIIVTNEEFENSLLKH
                     NIALIPEAHALLSYLMQCIYNNVIDSQEARTFEYGGQTVVLRLFDAISSNPASLLDNK
                     NRVLFSQAPDEVAAYRVVCDYLANMTDEYAYRMHERLFGFNTRTIFERL"
     gene            24428..>26710
                     /locus_tag="ACF2SP_001041"
     CDS             24428..>26710
                     /locus_tag="ACF2SP_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358835.1"
                     /translation="MPYTLTLLGTDTQFSPNRLEGAYDKAETLSYVSTLVSNNQPQDR
                     TFPTDEIVKYRTSKIAVVDGPTTLGTEVGDRIARGVEAILEAISRGETDISIIAHSRG
                     AVEAILVAHELERIQSLVEKGNFNRYQLTNSECRYTNRAMNRDANHTKAFDSLDLEKI
                     ANNIGRVKISMFNIDPVPGGNYMGITHASSLAWRDPRFYSIPKIVKEYEQYTYENERT
                     RCFKPIVPKCASTETHFKLHTLPGHHGTGSGNLLDQQRGNIPSDKTTEHVQELVVVKL
                     LDFLTRNNVTIRPKSSEEHDPFANITDQLFNGESIDRGKLKSLFFNLYEEISRNREAY
                     QHFNRTSYAVLGQEQAILRRIWNITDQRIVHYQAHNDTYLDTVVPPVPGGHFLNYEHA
                     RLHLNQELGLEEGRPLSETINNAVDRLISVCRHTHQLKDLRVSGAAIDPTASVLLDKI
                     APTLDTREGFDLFLEGLGMLIDEVRRPYLQGELELINPEERASLYSAIVRAFESFNKY
                     THDNPQNELAKSILSSLNSNLESTLETKRKKLDERYETLSMKLRGKGFLTALQNRIKE
                     IKTNLNEKSTGLDSSEYELDLKLQELLIQTEKLSNSRVEEIKETFEQALQSFREVRFT
                     SELARNTQEWICLVLDEAIDESLNYSVESLMSEVIKSYNELDNFKKTLPDFKILYDSL
                     NYSEWESNLERKRDHMVHLAARYIAHEGLDLEKDIKPFFPHDSAIYLQIEALAIGLGA
                     RNPHIIRLLDENRLNLEKIDE"
BASE COUNT         8348 a         5553 c         4562 g         8247 t
ORIGIN      
        1 aagttagtgg ctaatgtaat tatttccagc cttgctttgg ttagtgtatg aatcgcgggg
       61 gttaaagagg attcaccacc tgcagcaata atcttttctt tcaaagccat aagttcaatt
      121 tgatgctgca gctctttcaa tgtgggaatc tcattttctg cttgaccatt aagaacagca
      181 ttttgaatca catgtttaat tttttgctgg acaagctctt tatttgcctt aattttacta
      241 gggtccaaac caaaggctga gaagacagcc atttcatgag ggttttgtaa gaatttttca
      301 gggtgagtaa tttgctgatt aacaaaagca cgctttaaaa caagagagaa gtcttcaaaa
      361 tactctttag gcaacctcat caaggcttgc ttaagcttag gaaacatttt aattactact
      421 tcttcttggg ctgttatctc accttggtta agttttacac ttaatttggc tatttcttgc
      481 tggtatcttt cttttgtcat tttcccttca gcataggcaa taatccgagt tttatctgat
      541 tctgtcatca ttcgagcact atcgcgaata tcagttccga ctatatccca gttactgatt
      601 attttttcag ccatttcttt actagtgctc ttctccaaac gatcataaag atctataaga
      661 gcttcacgat tacatgctga acgaacattc actacaaatt gatccaacgt ctcaacattc
      721 ttcgagtgat cagctaatgt tagcttgtct cccatatgct caataacatg ttgaatatct
      781 ctaactataa ctggtgctaa tttgcctaca ttgctaacac cttcttgatt gagtatttgt
      841 tttaccactt gagtagggtt tgtattcact aatgaaagag attcaagaac gttatcttct
      901 gctgcaattt tctttaattc ctccgaaaga acttgaataa attcttgatt atttcccttt
      961 tgggaactca atacagactg ggctcgctct acggcaacat caaaaccggc tggtgaacct
     1021 ttccattctt ttcttttttg ctcaatcatt atgattcttt gttgtccaat aagagatatt
     1081 ttttccataa actcatttat ttcagacaga gagcgtaatg gtaaggtatt gtcatcaaaa
     1141 cgtttactac taggccagta agttgcatat agctgttcaa aggggatttc ataacaaagc
     1201 tctgcacttt ttttctttaa ctcatccata atagagctat ttattatgac ggctaactcg
     1261 tcaattgctt ttcgatggat ttgttgccaa tattcaaaat tgtcaggatg aatgcaaagc
     1321 ggcataccat ctggaattaa ctcatactcc ttaaatatct ctttaacaga ggaaatatta
     1381 aagacattga atcgatcgga gatcttattc gaggtaacgc tctcccaaaa agtcgctaga
     1441 tatgtatccg tttctgattc cattaattga agtgcattta atgccaatat ttcttgctta
     1501 aattgcctaa gattagcctg cagttgtagt ttatattcgg ttatactggg aagagcatca
     1561 ggattgtggg cataaaaaaa tcgaactgtt ttctgaaaga gccaatttct taaaacctgc
     1621 atattaaaag agcttaattg ctcgtcattt acatcttgag gccattcacc atcctttttc
     1681 caataatcac tggtgttgca atattcacat aattgtctta ttgaaatcac atcttctaat
     1741 gatccgaatt catcacgaac tgcactgatc acccatgcca ttttatcgtc acattcaact
     1801 ttgatttttt ttacgtaatc taagaaacgg ttagtttcaa tggtataact atcatcctgt
     1861 gcaataagaa tgggttgccg ttcctctggt ggaagtagac tgaaatccac ctcaacttct
     1921 gtcggaaaat cataatagta aacatattgt ccaccgaaca cagatttaga tgtgttttta
     1981 gtcatattga ctcgatttat gagtgaaaag taattatatt atacaataga tattatattt
     2041 gatcacatat gcaacgagtt agattttgaa atatgtttat ttgacagatg catactgaca
     2101 acatctcaac gattaccatc agatacctgt cgagctactg ctccatttaa aaacaaactt
     2161 tattataatt ttatcaaaaa caatggtttt ataatcaaag aaataaaata tctctaaaat
     2221 gtatctacta ccaaacatct cccagatttt ctcaatatag ctaactctat ttgaatgcct
     2281 tcacttaata tttctaaagc aatttataga atagagctct tttataacta gcatctttta
     2341 ctctatttat acaagaactc tagttttgct gaattgtagt gaatgatttg accaggcagt
     2401 gaatcttgga caaatattaa tgagattaaa aataatctta taaacttata cactagtaga
     2461 gtggctttct ctccaaaaag tctggagaga aaaccaaaca tctgtgttat tcttaattga
     2521 cagggctgat tgattaagga acagacattt ccatgttcta tttccttact atgttcgcat
     2581 gatgaagcaa gttattaaca tataaatcat aatccatcat tccatagcta gcttctattt
     2641 gctgtatcaa gctatctctt tgctcttgat ccagagagct taattttcca tcattaattc
     2701 gctttaactt aacaacaaca tagtcaccgt tgtctaaaat gactccgtca cggctttctg
     2761 gacgtaataa attaaatgct aaatcattaa ttaaagaatt tgctttatca ctgtctctgg
     2821 aagcattctc taccgaatgc cattcgaatt gattgttagc aattaattct tgctgttgct
     2881 tatcttcaac agggtgaagc aaactgctac caatttcttt tgctttagca tctccatact
     2941 gttttgcaag tattttttta atctgatctc gcacttcatc caaggtttgt tctcttgaag
     3001 gtatatgttt cttcactcgt aaaactatta cagagtcatt atcaatctgt ataggctcgc
     3061 tattattccc tagttctaaa acatcagtgc tgaatgctgc atggataacc tgcttatttt
     3121 tggtaatagg ttgcttgcca ccatcacgag aaaaaggctc tgtatgttga attttcagct
     3181 gtaatgaatc caaaaccgga tcaagtgaat caggagtttg ataacttaaa tctgttaatt
     3241 gctctaaaac ttgagcatat tttgcctgag ccatatcact ggttagttga tccttcataa
     3301 tgttttcaac ttcttgcaaa gatttagttg taactggctt gtaagcaatc agcttaaaaa
     3361 tttcataacc atattttgtt tgtactggta tagaaatttg ccctggtcta gttaaattgg
     3421 aaagtgctct gccatattca ttttgcccgc cagtaatcca gggaagaata cccttattgg
     3481 ttatagataa cttgtcatca gatttagaag caacatattt atcaaattgc tctggatgct
     3541 tttttaaatc gctataaacc tcatcggctt tttgcttaat tgaatcttgt tcttccttgg
     3601 tggcattttc tggaactgca aataaaatat gggcaaccct ccattgagca ggagttaggt
     3661 agttactttt gttttcttca taatagcttt taatttggtc gggtgatatc tttatttgtg
     3721 acttgatatc atgcatagac aaaagcacat aatccaatgt aacttgctct gaagtcataa
     3781 attttttatg gtgttggtta taataactct tgatatcatc ttctgaaatc tttgcctgtt
     3841 gttcaaagcg atcagaaggt accgtcaaat aatcataatc cctggtctgc atgtacaatc
     3901 tgacaaaacg ctttatttca tctgataaag caaatgatgt tcccataaac gcaaaacgtt
     3961 gttgattaag caacatgcct tgccttactt cattctggaa agtctctgga gtaaacaaag
     4021 ctccactcaa agcttgttga tatcgttgtg ctgaaaaatg cccatcctcc tggaattgag
     4081 gaatttgcac tatggccgca ttggcttgtt ctggactaac ctcaaatcca tatttcctgg
     4141 cagcctgaat actcacctca ttcgttatca tttgatttaa tacttgattt tgcagatttt
     4201 tttcatcagc ggcagtcatc tgtggtaaat cttgctgagc acgagtacgt ctgtagttcg
     4261 tttcaaacgc ttgcatggta attggtttat cattaactat tacctttgca tcggatattt
     4321 gtcgagattg aaaataataa tcaacaccaa aaagtgtaaa ggtaatagca attaatataa
     4381 taactaacca cgctactaca ccctgaatgt gttcatttaa cttctgcaac atgtcccgag
     4441 atccattttg atattaacaa tgcttgatga aaacgattct accataagct gataaaaaaa
     4501 acgcgccttt cagcgcgttt atctggcgga gtggacgggg ctcgaacccg cgacccccgg
     4561 cgtgacaggc cggtattctg accaactgaa ctaccactcc atttcttggt gggtgctgta
     4621 gggatcgaac ctacgaccct cgccttgtaa gggcgatgct ctcccagctg agctaagcac
     4681 cctgaaaact taagcttgtt gtaccgcttc tttcaggttt ttacctgctt taaatttggc
     4741 aacccgtgac gctttaattt ggattgtttt tccagtctgt gggtttctgc cagtacgtgc
     4801 tgaacgatta cctgttgaaa aagaaccaaa accaggtatt acaacttgat cgcctttctt
     4861 taagacatca gttacagtag ccataaaagt ttcaagaact ctgcttgcgt cagctttggt
     4921 caaacctgaa ccgcttgcta tagcatcaac taattcactc ttattcatct tttccccttt
     4981 acagttaatc atccatattc cggagctgat ttaaacagat tcaactgctc cagtcaagtt
     5041 cgtacatcct tgcagagccc gccagtagcg ggccacgtag agaaatatac tctgtattaa
     5101 tgtgcgtgta aatcattatt tttaattttt tttgatcttt tattcaatat agtgccaggt
     5161 tgtatatttg ttggctcttc cacccatgga ttgcgttgca aagccagttc caaaacctgt
     5221 tcaatggtgt taacaggatg aattgtcagc ttgcggagca cattatcagg gatttcctca
     5281 aggtctttta cattctcctc ggggataatg acatgcttga ttccaccacg atgtgcagcc
     5341 agcaattttt cttttaatcc tccaattggt aaaacttgcc cacgcagagt aatttcacct
     5401 gtcatggcga catctgcctt aactgggatt tgtgttacta tggagactag taccgtgcac
     5461 attcctatgc cagcgcttgg gccatctttc ggtgttgcac cttctggtac atgaacatga
     5521 aaatcatgtt tctcataaaa atcatctggt aaaccaaaag ctttagcgcg gcttcttact
     5581 accgtcatag ctgcatggat agattcttgc attacctcac cgagctgacc agtatgagta
     5641 actttaccct tacctggcat aatggatgcc tctatagtca gaagttcacc tccaacactt
     5701 gtccaggcta aaccagtaac ttgacctatc tgatcgtatt gttcagctaa tccataacga
     5761 tattttttaa cgcccaaata cttagtaatg ttgctaacag ttatactgat tgtttttcct
     5821 ttttttccct ttttgcttga gagtatttct ttaacaactt ttctgcatat actcgctata
     5881 tccctttcaa gattacgtac tccagcctct cgagtataat aacggataat atctctaata
     5941 gccccttcgc taatatggat ttcttcatta gttaaaccat ttaacagcat ttgtttggga
     6001 accaaatatt tttcagcaat actgactttt tcgtcctcag tatatccagg caagcgaata
     6061 acttccattc tatccaataa cggggctgga atttctagcg aattggcagt tgcaataaac
     6121 ataacatcac tcaagtcata atcaacttcc aagtaatgat cactaaatgt atggttctgc
     6181 tcaggatcca atacttccag taatgccgat gccggatcac cgcgaaaatc cattgccatc
     6241 ttatctactt catccagcat gataagagga tttttaactc ctgccttgca taatttttgg
     6301 ataattttac ctggcataga acctatatat gttcttcgat gaccacgaat ttctgcttca
     6361 tctcttaccc cacccagagc gatgcgtatg aaagttcttc cagtagcatt ggcgatagat
     6421 tgtccaagag aagttttacc tactcccgga ggaccaacta gacacaatat cggtcctttc
     6481 aatcttttta ctcgttgttg tacagcaaga tattcgatga tacgctcttt aacctgctcc
     6541 agaccatgat gctccttatc gagcagcttt tctgctttta gcaaatcaaa ctgaattttg
     6601 gtacgcttct tccatgggac catcagcatc caatcaagat aattacgaat aacagttgct
     6661 tcagcagaca tcggagacat catttttaat ttatgtaact ctgctaatga tttctcttta
     6721 gcttctttcg gcattccggc ctttttgatt gaattttcca attgttcaat ctcattgcct
     6781 tcctcaccca tttcccctaa ttctttctga atagctttga tttgttcgtt tagatagtac
     6841 tctctctggc ttttttccat ttgacgtttg actcgacctc ttacacgttt ttccacatgt
     6901 aacagatcaa tttcattttc tatcgccgcc attaatttct caagtcgagt tcctacatcc
     6961 atagtttcaa gcaactcttg cttatcatct actttaagcg ttaaatgtgc ggcaatggta
     7021 tcggccaagc gacctggctc ttctattcct gccaatggag acaagacttc tggtggaatt
     7081 ttcttattaa gctttatata ttgttcaaat tgcgacatca aggaccgcat tagaattcca
     7141 atttcttgct cttgaattgt tgagccaact tcctcaatgt attcaagtgt cgcttcgagg
     7201 taacctttat cctgagtata ctccttgaca cgcgcacgct gctcaccttc gacaagaact
     7261 tttacagttc cgtcaggtaa ctttaacaat tgcaaaacac tggatacagt tcccacttga
     7321 tagatatctt ctgggccggg atcatcatgc gccgatttac gctgcgcgac taaaaaaatt
     7381 tgtttattat caatcatcgc tgcttcaaga gctttaattg acttgccacg cccaacaaag
     7441 agaggtatca ccatatgcgg atagacgaca acgtccctca acggtaatac aggcaaagct
     7501 gaagatttca cagtctcatt tgatattatt tcattttcat tagacataat gacccttatt
     7561 caaaggaaaa tttcttggtt acagtttctt atactattac ttatgagtat acagatattg
     7621 gtgctggtga acaattcgta atgtgagagt tagtctatcg ctattaagat gactaaattt
     7681 gttattttaa gctacaaaat cagctttaaa tctattttgt gaatgaaata taaaatgatt
     7741 tctgcaacaa tttaaattat tgcagaaatc aacatcttac ttaatcttta ctaccagacg
     7801 cacttttttt ctcatcctgt tcataaatga gaatcggttt agatagtcca ttcactacac
     7861 tctcatcaat aacaatctta ttcacgcctt ccaaggaagg taagtcatac atggtgtcta
     7921 aaagaatatt ttcaagtata gatcgcaaac ctcttgcgcc catttttctt tcgagggcct
     7981 ttttagcaat agcaatcaaa gcttcgtctc taaactcaag ttcagaacct tccattttga
     8041 aaagtgattg aaattgcttt gttaaagcat tcttcggtct cgttaatata tcaatcaatg
     8101 ccgcttcatc aagttcctgt aaagtggtta caacaggcaa acgtccaaca aactcgggaa
     8161 ttaacccata tttaattaaa tcatctgatt ccaattgacc taaaactttt gaagcttcgt
     8221 cattactgct ttttttgctt tttaattgag cagaaaaacc tatgcttgat ttatcgctgc
     8281 gttcgcgtat tactttgtcc aaacctgcaa aagcgccacc acagataaat aaaatatttg
     8341 atgtatctac ttgcaaaaat tcctgctgtg gatgctttcg cccaccttgc ggtggaacag
     8401 atgcaacagt accttcaatt aatttcagta aagcttgttg aacaccttct cctgaaacat
     8461 ctctggtgat agatggatta tctgatttgc gtgaaatctt atcgatttcg tcgatataga
     8521 caatgccttg ttgtgcctta tctacgtcat agtcacactt ctgtaataac ttctggatga
     8581 tattttcaac gtcttcacca acatatccag cttcagtaag ggtcgtggca tcagccatgg
     8641 caaaaggaac attaagaatc cgcgccaggg tttgagctaa aagagtctta ccactgccag
     8701 taggtcctat aagtagtata ttacttttac cgagttcaac accatcctcg cttttatgct
     8761 gtaatctctt atagtgatta tatactgcta ctgataaaac ctttttggca tgttgttgcc
     8821 ctattacgta ctcatcgaga aagttagata tttcttttgg tgtcggtaga cgtgcctcag
     8881 tttcttcatg agtttcatgt gtttcttctc taataatatc attacatagc tctacacatt
     8941 catcacaaac aaacacagaa ggtccagcaa ttaatttttt tacctcatgt tgactcttgc
     9001 cacaaaaaga gcaatataag accttatctc catttccgtt accggattta ctcataacca
     9061 aaccccttct tacagctatt actaattaat agtaattaca tgtaaaattt tagacaatca
     9121 gataatattg cgccaccaca ttactatcaa accaacttat attatactca gataaactag
     9181 gagtatctag caaagttata agtctgtaga atgcccagca acttgtctgt catagagaac
     9241 tttatcaatt agaccatact ccatagcctg agtagcgctc ataaagttat ctctttctgt
     9301 atcacgcatg atttgatcag gtgttttttt agtgtgttta gccataatat tattcaaacg
     9361 ttccctaact gccaaggtct ctcgagcatg aatttctatg tctgttgctt ggcctctata
     9421 gccacccaag ggctgatgta tcataactct tgagttaggt aagcagaatc gtttgccttc
     9481 agcaccagca caaagtagca aggcagctgc acttgctgcc tggcctatgc ataatgtact
     9541 tacatctggc ttaataaatt gcatggtgtc gtagatagca agacctgcag taacaactcc
     9601 accgggagaa ttaatatata gagatatatc tttctctgga ttttcagact ccagaaataa
     9661 taactgagcc acaaccaggt tagccatatg atcttctact tcccccaaga gaaaaataat
     9721 tctttctttt aataatcttg agtaaatatc ataggaacgc tcacctcgcg aggtttgctc
     9781 aattaccatt ggtattaatc cgcttgcatt acggataata ttatctgaat agcctggcat
     9841 gcttactctc cttttttctc agtgcccttt ttgggattca tcactgaatc ataatccata
     9901 tttttatatt taagtttcgc atcttctgct attttatcag caaccatatc ttccataact
     9961 aatgcttcaa cctcagccat atgttctttg ctgctttgat accatgcgcg aagctcatca
    10021 ggactttcgt aagcactggc aaatttatca atcatagcat taactttatc gttgtctgca
    10081 acaatttcgt gtttttttac atactcagca aaaagtaagc ccaggtgtac acgtcgcttg
    10141 gcttgttctt caaatagctc ccttgggaaa tcaggaattt tttcatcgtc tttatgttcg
    10201 tgaccaaaca atcgatgata catatcatgc tttaaatgct caatttcttt atctatcaaa
    10261 gctattggca actcaacatg attcacagac attaatgaat caaacaattt ctctctgttc
    10321 atcatgttga cgcgacgttc caattcacgc gccatatttt ctctaatatc ctttttaaga
    10381 gactctatac caccttcttt tatattaaat ttctccgcaa atgcctcatc caaagcaggc
    10441 aacttgcctt ccataatctt tttgatagtg atcttgaacg ttgcttcctt cccagccaaa
    10501 tctttatggc cataatcttc cgggaaactc acttttatat caaacggttt gtcaattttc
    10561 ccaccaacaa tgccatcttc aaatccggga atcatcgaac cagaaccaat tacaagctca
    10621 tatccttcag cactgccgcc ctggaatggc ttatcatcca aaaatccctg aaaatcaata
    10681 acaactttat cacctttttt gaccgcatga gttacttcat gccactcttt gttttgttct
    10741 cgcaattttt caatcatgtt gtcaacatct ttatcagtga cttcagatcg aattaattcc
    10801 actggagctt gattgagttc gacaatttca aaaacgggca taacttcaaa tacagcagtg
    10861 tatttaaaat ccttacctgg ttcgatttct aaaggctcaa catgaggata acctgctgga
    10921 accaattcat ttttttgtaa ggcttcatat aaggtagatt ggaccatttc ccgggcaact
    10981 tcttcacgca cactatcaga gaaacggctt tttacaacat taaaagggac tttgcctgga
    11041 cgaaaaccat ctattttcac tttgcgagca agatttctga gacgcgagct cacctcttcc
    11101 tcaactttct ctgtaggtac agaaactgta accttacgct ccaaaccttc tagggtctca
    11161 acagaaactt gcattaattc acctctcgga agttatcatg aaatggtgcg aaaggagaga
    11221 ctcgaactct cacgggtcgc cccgctggaa cctaaatcca gtgcgtctac caattccgcc
    11281 actttcgcaa tcaggttgga ttatcaatca gtaattcagt cttgtaaaga ctcagatgga
    11341 atctttagcg gtagactcaa aattatatct gttacaaatg gggtgaacga tgggactcga
    11401 acccacgaca accgggatca caacccgggg ctctaccaac tgagctacgc tcaccaaaat
    11461 tactcttaac agggcttacg aaacttccca aattatacgt tcataaattt tcaatatagc
    11521 tacttgcaca aaataggttt ttattaaaaa ttatgcaacc atggagggtt tcgtaagccc
    11581 tgttccagcg ataatactgc aaaagcaagg cggctggcgc gcccggcagg attcgaacct
    11641 gctaccctcg gcttagaagg ccgatgctct atccagatga gctacgggcg cttaaatggt
    11701 cggggaggag ggatttgaac ccccgacatc ctgctcccaa agcaggcgcg ctaccagact
    11761 gcgctacacc ccgatacccg tcccaaggac agagcgcaga taatactagg tgctatcatg
    11821 aaggtcaata tcctttagca tataattttt atttattata tagtctgaat tccaatctga
    11881 atttcttccg tacttttagt caatttattg ccctttacag gcacttacgt agctgcccca
    11941 acactataat ttagaataac gcatgccctt atactaatgc actatcaacc tcatgactta
    12001 aatcagcaat taggttagca taatgacata tcagcagata agttatgatc atgaaaacaa
    12061 aactattacc cttgtttgat aatctcaatc acatccataa aaatcatgaa aatgtgatat
    12121 tacctgatgc gcgatttcag aatgatttca cttattgctt aagtttttta aaaagctata
    12181 taggaagcga aggcacattc aacagttaca gaagagaaac agaacgtttg ttacagtggt
    12241 catggcttgt caaaaactct actattgatt ctttaaggcg tgacgatatt gaacaataca
    12301 tccatttctg tcaaaacccg cctaaaacat ggattagtct aaaaaaagtt ccacgattta
    12361 tagaaaaaaa tggtaaacgg atacctaatg aagagtggcg accttttgta gtgacactaa
    12421 gtaaggtttc caggaaaaat gggcatcttg catcaataga tcaattcaag ttatctcagg
    12481 gagccgtgca agaaatattc gccatcttaa gtactctatt caactttcta attgctgaag
    12541 aatatctgat atccaatcca gttgccctta ttaggcaaaa aagcaaattt attcgcaaaa
    12601 gacagcataa cgcgcctgtt agacgattaa gtctggttca atgggaagct gtcctgagag
    12661 cagctgaaag tttggctgaa gattcccctg aaaaacatga acgcactcgt tttattttaa
    12721 gtctgttatt tggcatgtat cttcgtatat cagaacttgc tgccagtgag agatgggtac
    12781 ccagtatgaa tgattttgcc aaggacagta atggccactg gtggtttacc accgtaggaa
    12841 aaggaaataa ggaacgacaa attgcggtaa gcgacgctat gctggaaagc cttacccgct
    12901 ggcgattatt tcttggactt accccacttc catcaccagc tgataattcg ccattactac
    12961 ctaagctgcg tggtcatggt ccaatgagtg acacagctcc tataaggcga attgttcagc
    13021 attgttttga cttggcagaa gaacaactgc gacttaatgg tttatcagag gaagcggata
    13081 gtctatccgc tgccacggtg cattggctgc ggcatactgg tatatccgaa gatgtaaaaa
    13141 tcaggcccag agagcatgta agagatgatg ccgggcacag ttcaagcgct acaacggaca
    13201 ggtatattga tatagaaaag caggcacgtt atcaatcggc acgaaagaaa ataattcagc
    13261 ccagcgaata atttattttt aaagaaaatc cggttttgaa cttatgcaaa tatccaactc
    13321 gcttacatga tcaatagtaa gcttaagcta atttatgata aaatggctga ttagttcaag
    13381 cttacggtct aagatgctta cattacgaca aattacttta tccagaggag ataaaacctt
    13441 actagataag gtcaatgtca gtctttatga aaaacaaaaa attggcctta tcggtcataa
    13501 tggatgtgga aaatcaacat tatttgattt tcttttaggt caattatctc ctgatactgg
    13561 agaatatcta attaatccac aactcagcat cagtcatttg tctcaacaca tacctgatag
    13621 caatgaaaaa gccttggatt ttgttcttgc cggagatcaa caatacatta aattgctaaa
    13681 gcgcctttca gaagctgaaa aaaccggcga tgatgcagaa gttttgcaat gtcacgaaga
    13741 actctctcat actggtggtt acagtaagcc cgcgcaagca gcagttatta tgtctggctt
    13801 aggatttagt ggcaatcaac agcaagagcc cgttaacagc ttttctggtg gctggagaat
    13861 gcgcttaaat cttgctcgat gcttaatgaa acccgccgat ttactcctgc ttgatgaacc
    13921 tacaaaccat ttagacatgg aagccatctt ctggctggaa cgctatttaa aacaaagccc
    13981 aagtactatt attcttattt cacacgatag agaatttctt gatgcctttg taactcatat
    14041 attacatata gaaaatcata atctgacact ttattcagga aactacagca gctttgaaaa
    14101 aattcatgcg caacaactgg ctctacaaca agcaatgcat gaaaagcaac aaaccaaaat
    14161 caaacatatg atgacttttg taactcgttt taaagcgaaa gcaactaagg ccaaacaagc
    14221 gcaaagtcga atgaaagcta tagaaaagat ggaaataatc gcagcagccc aagccgactc
    14281 ccctttttct tttgattttt tcccttgtcc tcgggcagga aatcctttaa ttcaatgcaa
    14341 tcaagttgat gcaggatata tcaatcatcc cccgattttg aaaaaaatca aactctctct
    14401 taatcccggc gatagaattg cgctactggg acctaacggt gaaggaaaat caaccttgat
    14461 taaaacgctt acggcagatt tgcccgtatt aagtggagat atccatcgtt ccccgcattt
    14521 acaaataggc tactatgctc agcatcaact tgagcaatta gactgtaaat taagcccggt
    14581 agaaacactg caaactctct cgccagaggt aagagaacaa accatacggg attttttagg
    14641 tggatttaat ttcagagggg atatggcggt acaatccatt cagcactttt caggtggtga
    14701 aaaagcccgc ctggcattag caagactggt ttggctaaaa cccaaccttc ttttgctgga
    14761 tgagccaaca aaccatttgg atcttggaat gcgctcagca attgaattag cgctgcaaac
    14821 ttatgagggc gccttaattt tgatttcgca tgaccgccat ttgttaaaat caagtgtgga
    14881 caatttttat cttatttatc aaggaaaagt acaaccattt gaaggggatc ttgatgatta
    14941 ctatctctgg ctgcaaacca gaaattcaga aaaggaatcc aaattagcta cttccccaaa
    15001 tgaatataaa gaaagaaaaa gctatcaaaa caaattaaaa aagctggagc aactcattga
    15061 gcactatcag aatcaattgc gccttcttga taagcagtta gctgacgttt ctctgtatga
    15121 agacacaaag cagaaaagca aattggataa tcttttgatg gaacgagata tcgctcattc
    15181 cgctttaatt aagacagaag aagagtggtt agaaatatgt catgccctgg aaagcctgga
    15241 gtaagtaaga ggttctgctg atcaatacca tttaaccttg taactgttcc aacatggctt
    15301 ttgctgccaa ctgctcagct tttcttctat taggccctgt gccataagct acctgactta
    15361 ctccctcaat tgtgcatgtt acatggaaga tttgagcgtg ctcatccccc tcaatcttgg
    15421 ttaggacgta ttcaggtaaa gcgaattttg atgcttgcaa aaactcctgt aattgtgtct
    15481 ttgcatcctt taaacaatga tttaaatccg gatcatctaa tcgtgaacta taaagcatta
    15541 aaataatctg ttttgcagca gtcattcctc catccaaata aatggctgcc aatatagcct
    15601 caagagcatc tgctaatata gatgtgcggc gaaatccacc gctccttaac tcaccttgcc
    15661 caagaaatag ataatcaccc aaacctattt ctctggctat ttctgctaac atttcacctt
    15721 taacaagaaa agagcgcaat cgacttaatt gcccttcact gtgaagtgga aaacgattga
    15781 atagctcatt ggcaataaca aaacttaata tcgagtctcc aagaaactca aagcgttcgt
    15841 aattatcgct accagcactg caatgcgtca aagcctgctt caaataggct atgttattaa
    15901 aatgatagtt taaacgtctg cacaatcttt ctaaattaat tttcactacc tgttattacc
    15961 tcaaaattat cttcaaaatt aaataataag ctcatattat aaattagagg tttgatgact
    16021 tgatatttta acttaatttt aaatttattg gggcctgaag gagtaataac cagctctcct
    16081 ggttttaagt cctctacccc attgatatct aatcgtttag tcacactttc ccttaaagtt
    16141 gtaacatcgg tcaagggatc ccccgacaaa gaagaaacag gtgttgaatt taatgctttg
    16201 attgaatgca aaatcgaata atgttgaata taaactggcg ctgcccgcat aacaagtata
    16261 ccagccatta tcaccactac ggcagtaaac agcataccaa tcaatgtcat tccctgttgt
    16321 tttctcatgc cctattccct caatgaatta atttgcctat ccttgaccag cgtacattat
    16381 ctgttttgcc attccaactc atccagacca gaaatgcttt tcctcttaaa tagctatcag
    16441 gcacaaatcc ccagaaccga ctatcggcgc tatcatcccg attatctccc atcataaagt
    16501 aattcccctc aggaacaact atatcaaaat ctacggctgg cgcatcaggt ctgacaaata
    16561 tgtcgtgcac cacaccgttt aaatcttctt tatactttgt cacagccttg ccagagcttt
    16621 catcggtagt gtactcaaca aaggtctgtt tcacctcttt gccattaata gttagttttt
    16681 tatcatgata tgaaatttta tccccaggca cgccaataac tcttttaata tagtcataag
    16741 atggttcagg tggccagcga aatactgcaa tttctcccgt tttgggatta gcaattggca
    16801 ctattttttt ctcccatact ggaagtctca gtccataaga aaatttatta accgctacaa
    16861 aatcaccaac caacaaagta ggctctagtg agcccgatgg aatacgaaaa ggttcaatta
    16921 aaaaagatcg caatagcaga acaataaaaa atacaggaaa aaaagaacgt gaatactcaa
    16981 tgattcgacc tggcttttgt tcaggcaaac gttttttaga ccaaatcaac caatccaata
    17041 aagcaatcaa tcctgttaca gcggataata tgaccaatat taaagcaaaa ttcataaatt
    17101 caatatcctc aattttaagc aaaagacaat ttaatcatga tttgacttat gtccagatgc
    17161 tgacgaaaag gtcaacaaat ccttattagt aaatgagcgg ctctactttt ttttatctgt
    17221 ttgaaacacg gccataaatg cttcttgagg tatttctaca tgccccacct gtttcattct
    17281 tttcttgcca gctttttgtt tttccagcaa tttccgctta cgcgtcacat caccgccata
    17341 acactttgct gtaacgtttt tgcgcaatgc tttcactgtt tgcctagcga taatatggct
    17401 tcctatagca gcctgaatag ccacatcaaa catctgtctt ggaataagct gttgcatctt
    17461 ctccgcaatt aattttccgc ggctatgagc ggaatctcta tgaacaatca cagcaagagc
    17521 atcaacacgt tcgctattta ttaatatatc catcttgacc aaatcagcaa tctggaatct
    17581 ttggaagtta taatccaaag aggcataacc tctactaact gattttaatc tatcaaagaa
    17641 atcagagact acttcgctca tggggatatc ataaacaaca gaaacatgac gaccactata
    17701 ggtcatactg acttgaacac cacgtctttc aacacataag gtaatgatag gccccaaata
    17761 atcttgtggc actagaatat ttgctctgac aattggctca tacatctctt ttatttgcgg
    17821 ggttgggggc aaatgcgaag gattatcaat taataaagtt tcccctttct gagttacaat
    17881 ctgataaaca acagtaggtg ccgtagaaat caaatcaagg ttatattctc tttccaatct
    17941 ctcctgaatg atttccatat gcagcatgcc taaaaatccg caacgaaaac caaatcccaa
    18001 agcctctgaa gattccggct cataaaataa agaagcatca tttaaactca atttagccaa
    18061 agcctctcta aatgcctcaa aatcatccga gctgactgga aacaaacctg cataaacttg
    18121 gggcttaacg cgttgaaacc ctggaagaac tttatcagct ggatttctat ccagagttaa
    18181 agtatcacca actggagcgc cctgaatttc ctttatacca gcaacaacat aacccacttc
    18241 accagcatat aaagcatcca gtttagtccg tttgggggta aaaataccta cttgatccac
    18301 ttcataagcc ctgcctgtgg acatcactcg cattttatcc ccttttcgta tggcgccatt
    18361 aacaatacga accagtgaga caactcctaa ataactatca aaccaggagt caataataag
    18421 tgcttgtaat ggggcatgaa catcgccttt cggaggggga atattcgcaa ctaatgcctc
    18481 taaaacatca ttaacaccca agccactttt agcactcact ctaatcgcat cttgagcatc
    18541 gagaccaata atatcttcga tctcggaaat aactcgctca ggttctgcct gcggcaaatc
    18601 aattttattc agtactggca atacggtaag agactgatcg attgctgtat aacaaactgc
    18661 taaagtttgc gcttcaactc cctgtgctgc atcaacaacc agaatagcac cttcacaagc
    18721 ggcaagagaa cgagatacct cgtaactaaa atcaacatgc cctggcgtat cgataaaatt
    18781 caataaatag gtctttccat ctttagctgt gtaattcaag gaaacacatt gcgctttgat
    18841 ggtaatacct cgctcacgtt caatatccat agaatcaaga acttgcgagc tcatttcacg
    18901 ctcagtcaac ccgccacaaa tttggataaa tcgatcagcc aacgtagact tgccatgatc
    18961 aatatgggca attattgaaa aatttcgaat tcggtttaaa tctttcaact gttctaacct
    19021 tcttgcaaat agcataagtc tatcttaaat gttcgcatgc agaaagtagg caaagcaaat
    19081 tcagtcattt ttatattctt aagagaatga aaattgctta ttcatttacc aatttcatgc
    19141 actgtattta agataaacaa aggccaaaaa tttgctatga ggctttattt ttactaaaat
    19201 tcgtagtttg caacgataca gggtacatga tgcgacaaat catctggcta ggtttcattt
    19261 cactcttcct tttatctttc actacctatt ccgacacaac atttacaaaa cgtaaagacg
    19321 tacaatcgtt tattaacatg atggtaacga attatcactt taatcgtaat cagttaacta
    19381 cagtaatgaa ccaagttaaa cttcaacctc aagtcatcga atctatggaa aagccctatg
    19441 aaaagaaaaa ctgggatgtc taccgagaac tatttcttac tcagaaaaga ttaaaaagcg
    19501 gattgcaatt ttgggctgaa aatcaaaaaa cattggaaaa aattcagaga aggtatgggg
    19561 tgtctccgga gatcattata gctattttag gagtagagac aatgtatggt gagcgtcagg
    19621 gtgaatacag agtcttggat actctggcta cactggcttt caactacccc aaacgttctg
    19681 cattctttac taaagaatta aaggagtttt tcctcctgtg ccgagaacag cacgtatccc
    19741 caacccaata caaagggtct tacgcaggtg ccataggaaa acctcaattt atgccaagca
    19801 gttatcgata ttatgcagtg gactttaata acaatggtcg aagagactta gtcaatgaca
    19861 atggtgactc tattgccagc atcgccaatt atttgcataa gcatggttgg aacttaaatc
    19921 aaggcatcgc tcaaccggcc aaaatctccg gatggcgata caaacaactt cgtatgaacc
    19981 ctaaaacagc aaattaccga tactcacaac ttataagcta tggagtaaaa ccaattacag
    20041 cgtcacaaaa tcatcctcct cgtgcaggag tcattgagtt agttacaagg aagggtaaag
    20101 aatactggat tgcctatccc aatttttttg taattacccg ctataactcc agccctcaat
    20161 atgctctggc ggtttattta ttatcccagc aattgaagca acattggtat actagtgcca
    20221 ctaaaagaca cagggcttat gtttaatgcc agctggaaat aataataatt actggtatat
    20281 actgaaaaat tgacagccat aacaatacag agtttgactc atggataagt gttttatttt
    20341 tagcaaacat ctcgatgata atggttgtgt atgcctgaaa atatctgact tgggtgagct
    20401 cattgctccc cctgaatttc gtaatttctt gcaaataaaa gatttacaaa aagaatccag
    20461 tacaattatt gtagaaacct gtaccagagc aatacttctt ggtcttgcac ttccatggct
    20521 acctgaaaga aaagccagaa ttgcaattcc ctatgcactt gaggataaag taactcaacc
    20581 agtcgaagaa cttcattttg cctttgataa attttattat cagaataacc aatatctcgt
    20641 tgttgtgatt gataaacaac gaatgagaaa gttaattcaa gtttttgatg agcatgggat
    20701 agagtttgat gcagtaactc ttgactggtt tgcgctagca tctcaagaat tgatcattag
    20761 tgaaaccgag ctattaatca ataacgaaga ttttaaaggt gtcctctcag gtgatttggc
    20821 tcaaacttac ctgaaaaatc atccacacaa tcccgctcat ctgtttcaag acagcaaaat
    20881 acctgtcacc tctccagcaa gccaaaatga agaacactca tacacttgga ttagtaaaag
    20941 attgataaaa tcaagaccac tcaatctttg tcaaggtgaa atacaacatg gaaaaacaac
    21001 cgattgggtt aaaaaaggat acctattggt tggcggatta tgcggcttct ggttaatttc
    21061 tattcttttt gttaattggc tttctttgca ttggctcaat agacaaatag atgaaatcga
    21121 ccggcaaacc gcagttattt atcgtgagtt ttttcctgat gccaaacagg tcatcagtcc
    21181 taagtttcga ataagtcagc ttcttggggg gagtactgca gaaaatcaaa ctcgattttg
    21241 gtttatatta aatcaattat ccaaagccat aaaagatagt ggtatcacag tagagcaatt
    21301 aaggtatcaa aacaaaacaa tatcaatcac attagtaagt tctgattttg aaagcttaca
    21361 aaaaattgaa aataaactaa aacaatcaca actcaaagtg aaacaaactc aagcctcaac
    21421 gaaagatcaa caagtggttg caactctgga gttaacgtga agacctattt aaacacgctg
    21481 aatgatcgag aaaagtggat ggttatagta gctggtgtat cactttttat ttatggctac
    21541 tatttattac tttacgcccc tctgagcaac caggtaaatc aaaaatccac tcaattaatt
    21601 gaaaaaactg aaacattgga atggatgaag caagtaagaa tgcaaaaacg ctctgccaaa
    21661 agaaaggaat ctgtagataa cagccaattg ttaactatgc ttgcttccca acttaaaaat
    21721 aataaaacac tcaaattccc ctatcaatta caacaaacag gttcggggga tgttcaatta
    21781 acctttgacg aagtcccttt tcaaaacttt atacaatggc ttgcaaaaat caacgaagtc
    21841 tattctatca atataaaaca atttgatgtg gagaaaaccc caacaccggg agtaacccga
    21901 ctgatgatca tactctctac aagttaaaag tgctattatg cattagtcaa gactataagc
    21961 cctgacctta gaataataaa atacagaata tcaaagtatt ttttaaatac atatagtttc
    22021 tcaagataac ctattcattc aaatttcctt ggatactccc ccaatcttta attccagcac
    22081 cctgtttaat ttcagggggt aatgattcca atgctttttg agctgcttct tgactatcat
    22141 aagttccata taaaccctta taatgagttt tgccattttg ataatatttc acttgagcca
    22201 ttctatcatt tttaggtgct ttgtagagtt tttgggccac ataagaagcc ttttcgtcat
    22261 cggcaatctg aatcgtatag ccttggggat tttgactgtt tacccaattt ctatctctgt
    22321 ccttaaagga tacaggagaa tgatactcac caacatgata tgaatcagga accacaacac
    22381 cttgaccaga ttgattgcca taattgtaat cacgagcatg ataatctgct tgaggataca
    22441 actgaccata atcataggta taaatttgat agcgggtagt gtatgtatcc tcataaacca
    22501 tacaagaaga aatattcatt acgctgatac ctagaatgat gtacctgatg ctaataccca
    22561 tcataacacc cccctgattt taacttttac ttttattctt atttgtagtt atctacaaaa
    22621 ataaggaaaa ctttaggaaa aagttttatg agttcattca gagttattgg atttctattc
    22681 aagccatcgt ccgaaaaaat ttctcttatt ctgcatataa caaaaaaata aaagtcatta
    22741 taaacaaccg tgattttcca gagactatgc aaagactcta ttgtttccca acatggtata
    22801 gaataaagta attagtttgt ggaataaggg caaaagatat gtggactcat agacgttctg
    22861 gtcaaaccaa tcaaagaggc tctcaagatc atagagatcc ctatgagcga gataggacaa
    22921 gagtaattca ctgtccagca tttagaagac tacaaagaaa aacacaaatt cttggaacag
    22981 atgagggcga ttttcataga acccgcctaa cccactccct ggaagtagat tctataggta
    23041 gaagtatcgt tcgcaacttg atgaccaatc aggaacacca gctgttattg tccagccttt
    23101 tgccaaatga tgatctcatc agcgtcattt gtttgcttca tgacattggc catccgccct
    23161 tcggacatgg aggagaagtc gcattgaatt atatgatgcg acattatgga ggatttgaag
    23221 gcaatggaca aactctacgt ttactgacaa aagttgaaaa tagttacggt ggttttggcc
    23281 tggatctaac tcgtcgtgcg ctgctaggta tcttaaaata cccggtaaaa cgagctaatg
    23341 tggtggctcc acaacaaccc cctattcatg aatcgattca taaaacaatc aaaatcaatg
    23401 actggttgcc tcctaaagct tattttgatt gcgagcagcc agaagtcgat tggttattat
    23461 cgcctttatc agataatgac agagaattat ttcagtctct ctccgtaaag cccaatggca
    23521 cccaagcagg taaagcagcc tatcataatt ttgattgctc tattatggac acagcggacg
    23581 atattgccta tggagtccat gacctggaag atgcaattca cttaagatta attaaccgtt
    23641 cacatcttga tactcctgag ttcaggcaat taatcagtga aacaaccctc tcgctacatc
    23701 gggagcgttt gcttaattct ctattttctc cagaaatctg tttaagaaaa caagctattg
    23761 gagaaatggt caattatttt attacctcta ctcaaatcat tgtaaccaat gaagaatttg
    23821 aaaacagttt attaaaacat aatattgcac ttatcccaga agcccatgcc ctcctcagct
    23881 atttaatgca atgtatctat aataatgtca ttgactccca agaagcacgt acctttgaat
    23941 atggaggtca aacggtagta ctccggctat ttgatgccat cagctccaat ccagccagct
    24001 tgcttgataa taaaaatcga gtgttatttt cgcaagctcc agatgaggtc gctgcttata
    24061 gagtcgtttg tgattatcta gcaaacatga ctgacgagta tgcttatcga atgcatgaga
    24121 gattattcgg gttcaataca agaacaattt ttgaaaggct ttaaaaatgg caaacatgta
    24181 aagtaatgac attaaaacaa aaccaaattg ttacttaata aaaacctttc aactaaaaat
    24241 ataattatta ttggggctat cccccaaatg tataccctat ttataccaat caattttttc
    24301 acaatttttt acaaagttat tcccttgctt aaataataaa caaactcact ccgagccgca
    24361 ttttttggtg tttttttatt tttattgtat atttattata atattagttc ttttgttgtc
    24421 tgatattatg ccatacacac ttactttatt aggtacagac actcaattct cgcctaatcg
    24481 cttagaaggt gcatacgata aagctgaaac tttaagttat gtatcaactt tagttagtaa
    24541 caaccaacct caggatagaa ccttccctac agatgagata gttaaataca gaacttcaaa
    24601 aatagcagtt gtagatggtc cgacgactct gggaacagaa gttggagacc gaattgcgcg
    24661 aggtgttgaa gccatactag aagcaattag tcgcggtgaa acagatatca gcattattgc
    24721 tcatagccga ggagctgtag aggcaatatt ggtagcccat gaattagagc gcattcaaag
    24781 ccttgttgaa aaaggcaatt ttaatcgtta tcaactgact aatagcgaat gccgatatac
    24841 taacagagca atgaatagag atgctaacca taccaaagca tttgattccc tggatcttga
    24901 aaaaattgct aataatattg gtagagtcaa aatatcaatg tttaatattg atccagtacc
    24961 tggcggtaac tacatgggca tcactcacgc ttcatcactc gcatggagag atcctcgttt
    25021 ttactcaata ccaaaaattg ttaaagaata tgaacagtat acttatgaaa atgagagaac
    25081 gcgttgcttt aaaccgatag tgcctaaatg cgcatccaca gaaacccatt ttaaactcca
    25141 tactcttcct ggacaccatg gaacaggctc gggaaaccta ctggatcaac aaagaggcaa
    25201 cattccctca gataaaacca ctgagcacgt acaagaacta gtggtagtca aactcctgga
    25261 ttttttaact cgtaataacg taactattag gcctaaatca tccgaagaac atgatccatt
    25321 tgcaaatatt accgatcaac tttttaatgg agaatccatt gatagaggaa aattgaaaag
    25381 tctatttttt aatctttatg aagagattag tagaaaccga gaagcatatc aacattttaa
    25441 tagaacctct tatgctgtgc ttgggcaaga gcaagcaata ctaagacgaa tatggaatat
    25501 tactgatcaa cgtattgtac attatcaagc tcacaatgat acatatttgg atacagttgt
    25561 tcctcctgta cctggtggac atttcttaaa ctacgagcat gctcgtcttc atttaaatca
    25621 agaattagga ttggaagaag gaaggccact tagcgaaaca ataaataatg cagtagatag
    25681 attgattagc gtatgcagac atacccatca gttaaaagat ttgagagtaa gtggagctgc
    25741 aattgatccc acagcttcag ttttattaga taaaatagca ccaacccttg ataccaggga
    25801 aggatttgat ctttttctgg aaggtttggg gatgctaatc gatgaagtga gaagacctta
    25861 tttgcaaggt gaattggaat taataaatcc ggaagaaagg gcatctctgt attctgctat
    25921 agtcagagca tttgagtcat ttaacaaata cacacatgac aaccctcaaa atgaactggc
    25981 aaaatccatt ctaagctcat tgaactcaaa tttggaatct acactagaaa ctaaacgcaa
    26041 aaaactcgat gagcgatatg agacgctttc aatgaaacta agaggtaaag ggtttcttac
    26101 tgctttacaa aatagaatta aagaaattaa aacgaatcta aatgagaaat cgactggact
    26161 tgactcttct gaatatgaat tagatctaaa attacaagaa ctcttaatcc agacagaaaa
    26221 attatctaat tcaagagtag aagaaattaa agaaactttt gaacaggcac ttcaatcctt
    26281 tcgagaagta cgttttacaa gcgaattagc aagaaacact caggaatgga tctgtttagt
    26341 tctagatgaa gccatagacg aaagcctaaa ttatagtgta gaaagtctaa tgtcagaagt
    26401 cattaaatca tataatgaac tagataactt taagaagaca cttcccgatt ttaaaatact
    26461 ttatgattca ttgaactatt ctgaatggga atccaatcta gaaagaaaac gagaccacat
    26521 ggttcattta gctgctcgat atatagcaca tgaaggcttg gatctcgaaa aagacattaa
    26581 accgtttttt cctcatgatt ctgcaatcta tctgcaaatt gaagcacttg cgatcgggtt
    26641 aggggcaagg aaccctcata tcatacgatt attagatgaa aatagattaa acctggaaaa
    26701 aattgatgaa
//
LOCUS       DAXLDT010000032        25815 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXLDT010000032 DAXLDT010000000
VERSION     DAXLDT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25815
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.032"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(72..1130)
                     /locus_tag="ACF2SP_001042"
     CDS             complement(72..1130)
                     /locus_tag="ACF2SP_001042"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFD2358836.1"
                     /translation="MTEEVLFSQEGQLGFITLNRPKALNALTLTMIMALQKQLSIWKE
                     DNSIKAVVVQAVPGNAFCAGGDIRWLYNAGRSKDSEQMQFFWHEYRLNHFIHHFGKPY
                     ISLLDGITMGGGVGISLHGSHPVASERFVFAMPETGIGFFPDIGASYLLNKCPGFLGV
                     YLGLTGNKLGPHDARKAGLVKQIVFAEQMQSMIEALKREDLSEDAFNLVDQCISSFAS
                     DAMPTEASQIKPLIDVCFSKPSVELIRESLQSTDGVWALGVDNTLLQKSPLSLKITLA
                     QIQKAKGLSLAECLKMDFDLASHFMKGSDFYEGVRSLLINKDKNPQWRPSSLELVTDA
                     MVVSYFESSSSGLELMIL"
     gene            complement(1133..1909)
                     /locus_tag="ACF2SP_001043"
     CDS             complement(1133..1909)
                     /locus_tag="ACF2SP_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFD2358837.1"
                     /translation="MNLIEQDIDNNGILTLTLNRPEKLNALSTDVLSALNELFFQAKS
                     NPKVKALLLTGNGKAFCAGADINRLAECNAQTGYEFACQGQEVFRLLETMGKPSLAAV
                     NGFAFGGGCELAISATLRIASNKAMFGQPEVKLGVIPGYGGTQRLARLIGKGRAMDLC
                     LTGRFINAETALNWGLVSEVVAPEDLLTQGKNILNGILSMAPLAVASVMEVIDHGYDL
                     SLTEALHLEAIHFAKVCATEDKKEGVAAFLDKRTADFKGV"
     gene            complement(1926..3080)
                     /locus_tag="ACF2SP_001044"
     CDS             complement(1926..3080)
                     /locus_tag="ACF2SP_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFD2358838.1"
                     /translation="MHFNFTDEHLAFREMASEFARNKLAPMADFWDEQGYFPIETLRE
                     AAQLGMAGMVVREDIGGAQLSRLDAALIFEQLATGCIPTSAYLSIHNMVASLVDRYGS
                     QEIRKKWGPKLTSMEVIASYCLTEPESGSDAASLKTRAVKEGEYYVLNGAKAFISGGS
                     VSDVYLCMVRTGDESHHGISCLLIEKNTPGLTFGKLEKKLGWRNQPTCMLYFENCRVP
                     IANRVGEEGMGFKIALNALNGGRVNIASCSLGGALACLRMSQSYMHERKQFGKPLTQM
                     QALRFYFADMLTDYEAARLMVYRAAAAMDNDDPNAPMYCAMGKRLATDVAFRISDKAM
                     QLHGGYGYLRDYQIERIFRDLRVHQILEGTNEIMREIIAKASLDEEYFIE"
     gene            3194..5020
                     /gene="recQ"
                     /locus_tag="ACF2SP_001045"
     CDS             3194..5020
                     /gene="recQ"
                     /locus_tag="ACF2SP_001045"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFD2358839.1"
                     /translation="MGTLAQPSFINPNALAVLKKYFGFDSFRPPQEKIIEDVISGEDV
                     LVLMPTGGGKSLCYQIPAIVRPGVGIVVSPLIALMEDQVTALKLQGIRAAYYNSSLTA
                     EEARNVLNQLHHNELDLLYIAPERLISTSFLDRLSECTISLFAIDEAHCISQWGHDFR
                     PEYACLGLLKTNFPDIPMIALTATADKQTREDIVTKLNYQPKKYVVSFNRPNIHYKVV
                     PKTNAVKQLNLFLQSDTQQSGIIYCGTRHTVEHLTAKLQELGFKARAYHAGLPHAVRR
                     EVQNLFRYDRIDIVVATIAFGMGIDKPNVRFVVHHDLPKSIEGYYQETGRAGRDGLPA
                     KALLLYDAADSARLRSWIMNIPLEEQRHIETNKLNHMLAFAEASHCRRQILLRYFDEP
                     SHTECKNCDVCDNPPQTADATEDAQKFLSCIYRLKQSYGLTHTIDVLRGSLSEKIKQS
                     GHEHLSTFGIGKEKSANYWKHLAWQLIHKDYCLQDMNHFNVLRLTPKAIPLLKGEESI
                     SLTIPNNDLNSSKKKGKQNPSFKPESNPLFEKLRALRRQLADEENKPPFMIFSDATLH
                     EMAQSKPKNSKQLLNVSGVGQHKLARYGHYFLEALNEFSEVE"
     gene            5030..5581
                     /locus_tag="ACF2SP_001046"
     CDS             5030..5581
                     /locus_tag="ACF2SP_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFD2358840.1"
                     /translation="MRKLLRPFYERDTVLVAKELLGKYLVHHDGLEEKIGRIVEVEAY
                     LGQHDLACHSSKGLTKRTKVMFGPAGYAYVYLIYGMYYCMNVVTEKEGIGSAVLIRAL
                     EPIKNIQDRTQGPGLLSKAMRIDSKLNHRDLLSNDFYIAEPNSPTDFTIIEKPRIGVH
                     YAKEWANELLRFYIKDNPYISKT"
     gene            complement(5776..6180)
                     /locus_tag="ACF2SP_001047"
     CDS             complement(5776..6180)
                     /locus_tag="ACF2SP_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFD2358841.1"
                     /translation="MKKLLSASVITLMITGCAAIPADPQAERVIASPNPAPKGCKYVG
                     QVVGNQGNFFTGGFTSNRNLEEGAMNDLKNKASKKGANYIQLVTNRAGVTGSMSGSFD
                     RHGGFMSGGSEQTNVTNLGNAYICPPKAIGLE"
     gene            complement(6236..6439)
                     /locus_tag="ACF2SP_001048"
     CDS             complement(6236..6439)
                     /locus_tag="ACF2SP_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HFD2358842.1"
                     /translation="MTDKVYQIVELIGTSQEGIEKAIDNAIKQVAKTHGKLDWFEVVE
                     TRGFIDDNKVKYYQVHLKVGCNV"
     gene            complement(6429..7115)
                     /locus_tag="ACF2SP_001049"
     CDS             complement(6429..7115)
                     /locus_tag="ACF2SP_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD2358843.1"
                     /translation="MNEWWLVILLVVLAVFACTLITYPLRRNRLVSLLLAPIIFIMVF
                     TGYYFWGDFARWQEYLHRNEAQELAQSMLKSIKSPQELIDKLKAKLDAQPESAKGWYL
                     LGRLYSSQKDNQNASLAFAKAHQLKPEDEQFAVNYAHSLWQLSNQQFNPDILEIFHTL
                     LKNNPRQPDALAMLAMHAYLSHDYEAAINYWQRLLQLAPAQSEEALAIRKAIAKAEQQ
                     INRGGKKSDR"
     gene            complement(7108..7509)
                     /locus_tag="ACF2SP_001050"
     CDS             complement(7108..7509)
                     /locus_tag="ACF2SP_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HFD2358844.1"
                     /translation="MRVFHFCFKVILLLLLVSVAFANSMYPLDSARKEAQFNHLLKDL
                     RCLVCQNQDLADSNAQLAKDLRFQVYELVKEGKSDSEIMDYLTARYGDFILFKPPVKS
                     VTLLLWFGPFLFLLIGFIIFWRTCFKRNSHE"
     gene            complement(7509..8042)
                     /locus_tag="ACF2SP_001051"
     CDS             complement(7509..8042)
                     /locus_tag="ACF2SP_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HFD2358845.1"
                     /translation="MKKIGWRVIPVAIFCLLCFFLWRGLSLDPHHLPSARIGKPLPDF
                     ALPELQNPDSTFSAMNFHNQVVLLNVWASWCEACVEEQVFMLQLAREGVPIYGLNYKD
                     NPEHARAWLVQWGNPYRLIGQDSEGKVAIDLGVYGAPETFVIDKKGIVRYRHVGIMNQ
                     KTWLTDILPLMRNLEKS"
     gene            complement(8039..9991)
                     /locus_tag="ACF2SP_001052"
     CDS             complement(8039..9991)
                     /locus_tag="ACF2SP_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFD2358846.1"
                     /translation="MIAELGLFFLVLALVASLMLALIPLAGLQFNRKDWIDSSRNYVV
                     LQFLFVACAYLCLTACFLMDDFSVIYVLNNSSLLLPWFYKFCAVWGGHEGSMLLWVAI
                     LSFWMILVCFFSSSLDTEMRARVLVILGWLSIGFILFLLMTSNPFLRQFQVLNTQGRD
                     LNPLLQDPGFLFHPPMLYMGYVGFCVAFAFAIAALWAGKVETSWSKWTRPWTLAAWCC
                     LTAGITLGSWWAYRELGWGGWWFWDPVENASFMPWLVGTALLHSLAVTEQRQQFKAWT
                     MLLAIAAFSLSLIGTFLVRSGVLTSVHAFAVDPQRGLYILGFLLFVIGGSLTLFLFRA
                     QTLQVNKHPGLLSRESALLLNNVFLVVIMLTVLMGTVYPLLVEGLGLGKLSVGAPYFN
                     AVFVPLMIPLLLLMGLGVRLKWQNDCWRDVFRKLNRIFLLSLILPVILLFCTSTEIHL
                     TALLGLILAFWVLLSTANAAYHKVRDRGVAGVGQAYWGMVLAHCGVAATVIGIAVSSA
                     YGVQDDVRMSPGKQVNLLDYQIGFINQTPLNGPNYKGTRAEFSIQYQNKTRKIFPEKR
                     VYTIGQMPMTESAIDVTPFRDIYIALGEPLSEDAWSVRIYYKPLVRWIWAGGFMILAG
                     GVLALTDRRYYRKRQVTVMSVREIKI"
     gene            complement(9988..10419)
                     /gene="ccmE"
                     /locus_tag="ACF2SP_001053"
     CDS             complement(9988..10419)
                     /gene="ccmE"
                     /locus_tag="ACF2SP_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFD2358847.1"
                     /translation="MTPVRRRKLFILLFALSVLSAAAALVLYALRQNISLFYTPTQIV
                     AGEAPAKHHIRVGGMVEANSIVRAKKGLDVQFKITDFENTIVVTYSGILPDLFREGQG
                     IVAEGEVTDNHHFHATQVLAKHDANYMPPQVKSALADKVKQ"
     gene            complement(10425..10583)
                     /gene="ccmD"
                     /locus_tag="ACF2SP_001054"
     CDS             complement(10425..10583)
                     /gene="ccmD"
                     /locus_tag="ACF2SP_001054"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFD2358848.1"
                     /translation="MNQFFEWLAMGGYSLYVWPAYGLVCVVLGMNILGIKWQRTRTRK
                     KLQRWFKR"
     gene            complement(10567..11322)
                     /gene="ccmC"
                     /locus_tag="ACF2SP_001055"
     CDS             complement(10567..11322)
                     /gene="ccmC"
                     /locus_tag="ACF2SP_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HFD2358849.1"
                     /translation="MWKILYQLASPKNFYNYAGRLIPWLAVSALTTMAIGMVWGLVFA
                     PPDYQQGDAYRIIFVHVPSAFLSMALYAWMGFLAILLLVWRIKMAGLLIHKVAQLGAC
                     MAFLALITGSIWGKPMWGAWWVWDARLTSELILLLLYLAILATYQAVKNKEDGDKIIA
                     ILALVGLIDLPIIHYSVYWWNTLHQGATLSVFAKPKIALSMLYPLLITLLGFFLYSLW
                     IILEKARNEVLFRERKQSWVKIQFEEESDESVF"
     gene            complement(11536..12216)
                     /gene="ccmB"
                     /locus_tag="ACF2SP_001056"
     CDS             complement(11536..12216)
                     /gene="ccmB"
                     /locus_tag="ACF2SP_001056"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFD2358850.1"
                     /translation="MNSPFQLFGKQFRREILIQVRQIRYLVNSCLFFLMLLFIFPLTL
                     RPELMLMRMFAPGLVWMAILLSMLLSLERLFQQDYEQGVLEQWLVSGQPLNLLVTAKV
                     MAHWLISVTPLLLLSPLIAILFSFNVWETCILLLSLICGTPAILFLCALAAAFGIGIN
                     QRGALMALILLPLTLPLLIFGSGTLSVAMQGLPVSGYLALLLAMSLLAVGFLPYAIAA
                     VIRISHID"
     gene            complement(12213..12815)
                     /gene="ccmA"
                     /locus_tag="ACF2SP_001057"
     CDS             complement(12213..12815)
                     /gene="ccmA"
                     /locus_tag="ACF2SP_001057"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HFD2358851.1"
                     /translation="MLDVIELDFDYHDQPLLQQISFHLPAGGLLHLKGSNGAGKTTLL
                     KLIAGLLNPEKGEILFERQSIKKDLCTYQKQLCFVGHRSGINPYLTLRENCLYDIHFS
                     PGAVGITELCRLFSLEHLIDYPCGLLSSGQKRQVALLRLWMSKAKLWLLDEPLVALDE
                     LSLLTIITKIQEHRAKGGAVLLTSHQDLPLNKADYEEYHL"
     gene            12963..13898
                     /gene="sohB"
                     /locus_tag="ACF2SP_001058"
     CDS             12963..13898
                     /gene="sohB"
                     /locus_tag="ACF2SP_001058"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFD2358852.1"
                     /translation="MEFLSEYGMFLLKCITLVIALLILLAGIFSMGRKTKPKLEITSL
                     NEEYQHLNALMNKEILGKKPGKKKKDKTKRPVLYVIDFSGDIKATQVEQLRDEVTSVL
                     SIAKPEDEVLVRLESPGGAVNGYGLAASQLQRIRDKKIPLTVSIDKMAASGGYLMACV
                     ANKIIAAPFAIIGSIGVVAQIPNFHRWLKKNNIDVELLTAGEYKRTLTLFAENTEKGR
                     KKFQEDLEKIHTAFREYVLKNRSQLDIDKVATGEHWIAKDAFDLRLVDKLATSDEYLI
                     EKMAEFNAFKLTVLAKLPIISKVLKPAMRLIHPWI"
     gene            14121..14402
                     /locus_tag="ACF2SP_001059"
     CDS             14121..14402
                     /locus_tag="ACF2SP_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358853.1"
                     /translation="MSDFKSKLPDLNELTSMTAKLFKGIKTSVSEIIQDYKQKRAQPV
                     STEEKTTENKPAESKAEPKTESKTTENKTEPKTESKPESNTEETQEKEK"
     gene            complement(14446..15861)
                     /locus_tag="ACF2SP_001060"
     CDS             complement(14446..15861)
                     /locus_tag="ACF2SP_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HFD2358854.1"
                     /translation="MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVN
                     PNPSVIVLGACASFEKMMDELDTLVKRFPKTPILVIDAVLSTSECADRNIVDCLSFPF
                     SYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
                     VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAI
                     TSRQGRFELANGGTLFLDEIGDMPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAA
                     THRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTV
                     RLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGENKPSCPEA
                     SGAERETLLQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMR
                     RTTLVEKMRKYGLTRPERMER"
     gene            complement(16057..16650)
                     /locus_tag="ACF2SP_001061"
     CDS             complement(16057..16650)
                     /locus_tag="ACF2SP_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358855.1"
                     /translation="MSNFRNFLVFILLFFSFCGQLTASSKQNLAFRNFWHPTYLGQRL
                     DYCTLDGKECGKEVANRYCQMLGYDYSTQNVIAYNVGLTNYLGSKAQCKGWRCNGFMT
                     IACAIGLSHTPPKPYHYREKKFAVPRYNDYRVDWCMERNKGCGKQAANSFCNRMGYMQ
                     AKDFVKQAQVSATKTIGSQELCFGNQCNAFKMIICYR"
     gene            17040..18056
                     /locus_tag="ACF2SP_001062"
     CDS             17040..18056
                     /locus_tag="ACF2SP_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2358856.1"
                     /translation="MKKYLTFIVSGLALLAIIWFIRHLWIIGTVKVVRPVYGTAVQAV
                     YATGTVEPTVMMPIASQNTARLVKLNADEGSEVVKGQILASLEDEHLQHNLKELKEHE
                     TLAKIRYERNAQLVKKGVISKDEYDRTLSEWQAASAAVRRIEAQISYLQLIAPADGRI
                     IKRDGEVGQLIPANQPVFWLSCCAPLRVSAEVDEEDIVLVRVGQKVLIRSDAFPNQIF
                     YGRVKSITPKGDPIARSYRVRINFSENNPLLIGMTAETNIIVHEKKNALLLPVSSISE
                     NKVWIVQDDGKLSQNTVTIGARGLKQAEILKGVNINDLVVLQPDNKLKNGQKVRFILV
                     KQEE"
     gene            18056..19306
                     /locus_tag="ACF2SP_001063"
     CDS             18056..19306
                     /locus_tag="ACF2SP_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2358857.1"
                     /translation="MKLFLKIALKHLLARKRQSLVSLMGIVLGVAFFLTISSLMQGSE
                     KDFIKRLVDNSPHITIIDEYRNPRIQPIQQLHTHGAIEIRSVKPLTEPRGIRGYEQTI
                     NNLSLTIPGIVISPVLIGQALISYAGKDYSVTLNGMIPEEIKKVSTIENYMTSGSIDD
                     LIVNPDGIVLGSELLRKLSLTKDDNITVTAPNGQIRTFKILGVFRTGRSDFDTNQAFI
                     SIKRAQALLNRPNRSNNIIIKLPNPYQAYDIAAQIEKQIGYKTISWQEKSEDLLNTLV
                     IRNIIMYSVVSAVLIVAAFGIYNVISTVVMEKHRDIAILKSIGFQKHDIQFIFIIEGF
                     LLGLVGCLLGLPLGSGLMYSLMQVQFKPPGSSELINMPLDWSYLQFVIATAFAMSASI
                     IAAVLPAHKAALVHPVDILRGGGM"
     gene            19303..19980
                     /locus_tag="ACF2SP_001064"
     CDS             19303..19980
                     /locus_tag="ACF2SP_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358858.1"
                     /translation="MNKIVIKTKELSRQLPGEVPVTLVKDINLEVREGEFLVISGPSG
                     SGKSSLLYLLGLLDRPSQGTIWLNNEDVSTYEEEKMAWLRLTQIGYVFQFHFLLPEFT
                     ALENVMLPMQRLGKLSLGEMKNRATKLLTSLGLEEQLSKLPKQLSGGQSQRVAIARAL
                     ANDPILLLADEPTGNLDTVSSTNVQNILKELAHEYRRSVIIVTHDMNFAATADRVIHI
                     VDGKMQE"
     gene            complement(20169..20927)
                     /locus_tag="ACF2SP_001065"
     CDS             complement(20169..20927)
                     /locus_tag="ACF2SP_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFD2358859.1"
                     /translation="MITRDELSLFLLDFLNCSQYQDYAPNGIQVEGKDKIKRICTAVS
                     ASEDVISQAIEQQADALLVHHGYFWKGENPVISGMKRRRIAKLLGHNMNLFAYHLPLD
                     CHPELGNNASLANLLLIGSHEMHKVNNTANLLWSGKLSKAMNSEQFSSFLEHKLGRYP
                     VHIAGNEKMIHSIAWCTGAAQDFIEEAYQLGVDAYLSGEVSERTFYQAMELGIQYFSC
                     GHHATERFGIQSLGVYLANYFDLEHWFIDSPNPI"
     gene            complement(20924..22192)
                     /gene="murA"
                     /locus_tag="ACF2SP_001066"
     CDS             complement(20924..22192)
                     /gene="murA"
                     /locus_tag="ACF2SP_001066"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFD2358860.1"
                     /translation="MDKLLINGGKALHGEVVISGAKNAALPIMAASLLASDHVTISNV
                     PHLKDITTMMELLGQLGAHLIVDEKMNVQVDSSQVNEFVAPYDLVKTMRASILVLGPM
                     LARFGKADVSLPGGCAIGTRPVDLHLKALRAMGADITVKNGYINARCKKGCLQGKRLM
                     FDTVTVTGTENVLMAAVLAEGITTIKNAAREPEVVDLANFLIQMGAKIRGAGTSTIEV
                     EGVESLNGGTYSVMSDRIEAGTYLAAGALTRGQVTVKKVRPDTLLSQLCKFEEAGAEL
                     TIGEDWVSLNMHNKRPQAVNISTAPYPAFATDMQAQFMAMNSVAEGSSTIIETIFENR
                     FMHVQELQRMGANIQLNGNTAIVHGVEKLTGAPVMATDLRASASLILAGLVAEGETVV
                     ERIYHVDRGYERIEEKLSLLGADIKRVSDR"
     gene            complement(22185..22430)
                     /locus_tag="ACF2SP_001067"
     CDS             complement(22185..22430)
                     /locus_tag="ACF2SP_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HFD2358861.1"
                     /translation="MLSNEEIEQRLNEIDDVFYVKVEGDGYQYQVTVVTDIFLKKSKV
                     ARQQWVYAQLKDFITTGRLHAISMKTWTKEEWGKQNG"
     gene            complement(22820..23101)
                     /locus_tag="ACF2SP_001068"
     CDS             complement(22820..23101)
                     /locus_tag="ACF2SP_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HFD2358862.1"
                     /translation="MEINNFKPGAELTFKSVVSVRDKLYKALMEDTRGRFCLDLSEVT
                     HCDSAGLALIIEARKLCKQNNKVFDVIGISPETQSLAEFCGVKTILQRS"
     gene            complement(23104..23712)
                     /locus_tag="ACF2SP_001069"
     CDS             complement(23104..23712)
                     /locus_tag="ACF2SP_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFD2358863.1"
                     /translation="MRVFKTILLVVLMTLSQAMVAQTSPIPMLERAANQIISILKENK
                     ARLKSNPEIIYKAVEENLLPNVDVAGMSRSVLGRQAWSKATEAERVEFSKAFTRLVIR
                     TYSSPLAQYSDETVQFLPLRGSLNSRFIRVNSVIVRSAGQNIPLSYSLVSKNGKWKIY
                     DISVEGVSLLQSFRSQFAQALQHSSISQVIKEMQQKQIKKAS"
     gene            complement(23720..24196)
                     /gene="mlaD"
                     /locus_tag="ACF2SP_001070"
     CDS             complement(23720..24196)
                     /gene="mlaD"
                     /locus_tag="ACF2SP_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFD2358864.1"
                     /translation="MKKQRYVDISVGIFMLLGLLALLVMAMKVSNITDFMSDKTYRVT
                     ADFTDIGGLKVRAPVTVAGVRIGEVSRIELQPGELNARVTMTLNGDKKIPYEDASARI
                     LTEGLLGSNYISIVPGFDEDGDTDHPYLRDGDTIAKTQEAIILENLIGQLLFNIKK"
     gene            complement(24197..24979)
                     /gene="mlaE"
                     /locus_tag="ACF2SP_001071"
     CDS             complement(24197..24979)
                     /gene="mlaE"
                     /locus_tag="ACF2SP_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFD2358865.1"
                     /translation="MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLR
                     YQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKFGASSQLGQLLALSVARELGPVI
                     SALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
                     FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVY
                     QGFECVPTAEGISHATTKTVVYSSLAVLGLDFLLTAMMIGDW"
     gene            complement(24972..25769)
                     /locus_tag="ACF2SP_001072"
     CDS             complement(24972..25769)
                     /locus_tag="ACF2SP_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358866.1"
                     /translation="MPDNWVEIKNLFFSLDDRCIFDGIDMVVKQGKITAIMGPSGSGK
                     TTLLRLIGAQLRPDSGQIYVNGQNIHQLSRKSLYETRRNMGLLFQSSALFTHLSVFEN
                     VAFPLREHTELNASMIRNIVLMKLEAVGLRGAAHLMPAQLSGGMCRRVALARTIALDP
                     VLMMYDEPFTGQDPISMGVLVRLIQRLNQLLHTTTIIVSHDVEETCSIADYIYLISGG
                     KIIGQGTPDELKRSIEPQIRQFMHGEADGVVPFHYPAKPYTEELLNA"
BASE COUNT         8334 a         5650 c         4508 g         7323 t
ORIGIN      
        1 tcagactgct ttcaaccata aggaagtatt catacgggaa atctattatt cccccaatca
       61 aaatccaaaa actaaagtat cattaattcc aatccggaag atgagctttc aaaataactc
      121 actaccatgg catcagtgac caactccaaa ctggacggtc tccattgagg atttttatct
      181 ttattaatca ataaagagcg aactccttca taaaaatcac tacctttcat aaaatgactg
      241 gcgagatcga aatccatttt taaacattct gctaaagata agcccttggc tttttggatt
      301 tgtgcaaggg ttatcttaag gctcagaggc gatttttgca gcaaagtatt atccacccca
      361 agagcccaca caccgtctgt gctctgtaaa gactctctta ttagttcaac actaggcttg
      421 gagaaacaga catcgattaa aggtttgatt tgacttgcct cggtgggcat ggcatcactt
      481 gcaaaagaag aaatgcattg atcaacaaga ttgaaagcat cttctgataa atcttctctc
      541 tttaatgcct caatcattga ctgcatttgc tctgcaaaga caatctgctt cactaaccct
      601 gccttcctgg catcatgagg acccaattta tttccggtta accccaaata gactcctaaa
      661 aaccctgggc atttgtttaa taaatagctg gcaccaatat caggaaaaaa accaatccct
      721 gtttctggca ttgcaaatac aaaacgctca ctggcaacag ggtggcttcc atgtaaggaa
      781 ataccgaccc ctcctcccat agtaatccca tccagcaaag aaatataagg tttaccaaaa
      841 tgatgaataa aatggtttaa cctgtactca tgccaaaaga actgcatctg ctcagagtct
      901 ttactacggc ccgcattata tagccaacga atatcaccac ctgcgcaaaa agcattaccc
      961 ggaacagctt gtacgacgac tgctttaata gaattatctt ctttccaaat tgacaattgc
     1021 ttttgcagtg ccatgatcat ggttaaagtt aaagcattta aagcctttgg tcgatttaaa
     1081 gtaatgaatc ccaattgacc ttcctgagaa aacagcacct cttcagtcat aattaaaccc
     1141 ctttaaaatc cgcggttctc ttgtctagaa acgcagctac tccttccttc ttatcttcag
     1201 tggcgcaaac ttttgcaaaa tgaattgctt ccaaatgaag tgcctctgtg agtgataaat
     1261 cataaccatg atctatcact tccattacac tggcaactgc taaaggagcc atgcttaata
     1321 ttccgtttag aatatttttt ccttgagtca acaaatcctc tggtgctaca acttcactga
     1381 ctaaccccca gtttaaggca gtctcagcat ttataaagcg cccagtcaaa cataaatcca
     1441 tcgcgcgccc tttgccaatt agcctggcca atcgttgtgt gccgccatac ccgggaataa
     1501 cacctaattt tacctcaggc tgaccaaaca ttgccttatt tgaagcaatt ctaagagtcg
     1561 cagatatcgc taactcacat ccgccaccga aagcaaaacc attcactgcg gcaagtgaag
     1621 gtttccccat cgtttccaaa agtcgaaaaa cctcttgtcc ctggcaagca aattcataac
     1681 cagtttgagc gttacattca gccagtcgat taatgtcagc cccagcgcaa aatgctttac
     1741 catttcctgt taacaataat gctttgactt tgggattgga tttcgcttga aagaataatt
     1801 catttaatgc gctcagaaca tcagtgctta aagcattgag cttctctggc ctattcaaag
     1861 ttaaagttaa gattccgtta ttatctatat cctgttcaat taaattcatc aataatcccc
     1921 tatgattact ctataaaata ctcttcatcc aaacttgctt tcgctataat ttcacgcatg
     1981 atttcattag tgccttcaag aatttgatga actcttaaat ctctaaatat tctttcaatt
     2041 tgataatctc gcaggtagcc ataacctcca tgcaattgca tggctttatc actaatacga
     2101 aatgcgacat ctgtagccaa gcgcttgccc atagcacaat acattggcgc attgggatca
     2161 tcattatcca ttgctgctgc agccctataa accatcagcc ttgccgcttc ataatctgtt
     2221 aacatatccg caaaataaaa acgaagggct tgcatctgag ttaaaggttt tccaaactgt
     2281 ttacgctcat gcatataaga ttggctcatt cgcaggcaag caagagcccc tcccaatgag
     2341 caggaagcaa tattcactct ccctccgttt aaggcattca gtgcgatttt aaaacccata
     2401 ccttcttcac caacacgatt ggcaatgggc acacgacaat tttcaaaata tagcatacaa
     2461 gttggctgat ttcgccaacc taattttttc tccagtttgc caaatgtcag accaggagta
     2521 ttcttttcaa ttaacaaaca actgatgcca tgatgagatt catcgcctgt acgtaccata
     2581 cataaataca catcactaac acttccaccc gatatgaatg ctttcgcacc attaagaaca
     2641 taatattcac cctcttttac cgcgcgagtt tttaaagagg ctgcatcaga tccggattct
     2701 ggctctgtaa gacaataact ggctatcact tccatactgg ttaattttgg cccccatttc
     2761 ttgcgaatct cttgactgcc atagcggtcg accaatgaag caaccatatt atggatggag
     2821 agatatgcac ttgttggaat acagcccgta gcgagttgtt caaagatgag tgctgcatcc
     2881 agacgagaga gctgagcacc tcctatgtct tcgcggacta ccattcccgc catacctaat
     2941 tgtgccgctt ctcttaaagt ttcgattgga aagtaacctt gttcatccca gaaatctgcc
     3001 attggagcca gtttgttacg agcaaactcg gaggccattt ctcgaaatgc caaatgttca
     3061 tcagtgaaat taaaatgcat agaccatcct tagatttatt gattttagtc attggattta
     3121 tttatgacgc tttcttaaaa aatgatatta ttgcgaattt ttagcgccca ggaaagataa
     3181 taagaataat tacatgggaa ctttagcgca accatctttt ataaatccaa acgcattagc
     3241 tgttttaaaa aaatattttg gatttgattc cttcagacct cctcaggaaa aaataattga
     3301 ggatgtcatt tctggggaag atgtactggt tttgatgcct actggagggg gaaaatcgct
     3361 ttgctatcaa attccagcca ttgtccgccc tggtgttggc attgtagtat ctcctttaat
     3421 cgcgttaatg gaagatcaag taaccgctct caaactgcag ggtattcgtg cggcttacta
     3481 caactcatcc ttaaccgccg aagaggctag aaatgtatta aatcaattac atcataatga
     3541 attggattta ctctatattg ctccagaacg tcttattagt acctcgtttc tggatcggct
     3601 gtcagaatgt actatctctt tatttgctat cgatgaagca cactgtattt cacaatgggg
     3661 acatgatttt cgacctgaat acgcctgttt aggtttactg aaaactaact ttcctgacat
     3721 tcctatgatt gctctgacag ctactgccga taagcaaaca cgtgaggata ttgttaccaa
     3781 gttgaattat caacccaaaa aatacgttgt ttcattcaat cgaccgaata tccattacaa
     3841 agtagttcct aaaacaaatg cagtgaagca attaaatctg tttttacaat ctgataccca
     3901 gcaatctgga attatttatt gtggaacccg ccatacagtc gaacatctta cagcgaaatt
     3961 acaagaactt ggatttaaag cacgcgctta tcatgcaggc ctgcctcatg cggtgcggcg
     4021 tgaggtgcaa aaccttttca gatacgatcg cattgatatt gtggtagcta ctattgcttt
     4081 tggaatgggt attgacaagc ctaatgttcg ctttgttgtt caccatgatt tacccaaaag
     4141 catcgaaggt tactaccagg aaacaggtcg tgcaggacgt gacggattac cggccaaggc
     4201 ccttcttctt tatgatgctg cggatagtgc ccgtttgcgg tcctggatca tgaacattcc
     4261 tcttgaggag caaagacata tagaaaccaa taaattaaat catatgcttg cttttgctga
     4321 agcgtcgcat tgtcgtcgtc aaattttatt acgctatttt gatgaacctt ctcatacaga
     4381 atgtaaaaac tgtgatgtct gcgataaccc accgcaaaca gccgatgcaa cagaagatgc
     4441 gcaaaaattt ttatcgtgta tttatcgatt aaaacaaagc tatggtttaa ctcatactat
     4501 tgatgtcctg agaggtagtt tatctgaaaa aatcaagcag tctgggcatg agcatttaag
     4561 tacctttgga attgggaagg agaaatctgc caattattgg aagcatcttg cctggcaatt
     4621 aattcataaa gattattgtt tacaggacat gaatcatttt aatgtgttac gcttgacccc
     4681 aaaagcgatt cccctcctaa aaggtgaaga gagtatttca ttaaccattc ctaacaatga
     4741 cctgaatagc agtaaaaaga aaggcaagca gaatccttcc tttaaacctg aaagtaaccc
     4801 attatttgaa aagcttcgtg cgttaagacg tcagttagcc gatgaagaaa acaaaccacc
     4861 atttatgata ttcagtgatg ccacattaca tgaaatggcc caatcaaaac ccaaaaacag
     4921 taagcaacta ctgaatgtat ctggggttgg gcaacataaa ttagctcgtt atggccatta
     4981 ttttctcgaa gccttaaacg aattttcaga agtcgagtga gattccgtta tgcgtaaatt
     5041 actaagacct ttttacgaac gtgataccgt tctggttgca aaagaacttt taggtaaata
     5101 tttagttcat catgatggat tggaagagaa aattgggcgc attgtagaag tcgaagccta
     5161 ccttgggcaa catgatttgg cttgtcattc atcaaaagga ttgactaaaa gaactaaagt
     5221 tatgtttggg ccagcaggct atgcgtatgt ttatctaatt tatggcatgt attattgcat
     5281 gaacgtagtc accgaaaagg aggggatagg ttcagcagta ttaatacgtg ccctggagcc
     5341 tatcaaaaac attcaagaca gaactcaagg accagggtta ttatccaaag ctatgcgcat
     5401 tgactccaaa ctgaatcatc gtgatttatt aagcaatgat ttttatattg ctgagccaaa
     5461 cagtccaact gattttacca ttatagaaaa acccagaatt ggggttcatt atgctaaaga
     5521 atgggctaat gagttattaa gattttatat taaagacaat ccttatattt cgaaaacctg
     5581 attaggcctc gtgcataaac tgtctctttc attttattgc cgcattgcaa agatttctgt
     5641 gatgctcagg gtatgtaaaa agcgtattat cgtattaatg agcccattat tattacatcc
     5701 ctttcttcaa gcatgtcctg tcaatccccg gatccaggct ttgtgcctaa tccggggtaa
     5761 tttcttgaaa taactctact ctaacccaat tgccttgggc gggcagatat aggcattacc
     5821 cagattagtc acattggttt gttctgaacc gccgctcata aaaccaccgt gacgatcaaa
     5881 tgaaccactc atggaacccg ttacaccggc tcggttcgtc accaattgaa tgtaatttgc
     5941 cccttttttg cttgctttat tcttcagatc attcattgct ccctcttcca aattgcgatt
     6001 ggaagtaaag cctccggtaa agaaattacc ctgattgcct acgacctggc ccacgtattt
     6061 gcaaccttta ggtgccggat tcgggcttgc aatgacacgt tcggcctgag gatctgctgg
     6121 aatagctgcg catccggtaa tcatcaaagt aataacagac gcagatagta gttttttcat
     6181 aattacacct ttttgttatc aacaaaacaa tgaaatgtta acaactcctg tttgattaaa
     6241 cattacaacc cacttttaaa tgcacttgat aatatttcac tttattatca tcaatgaaac
     6301 ctcttgtttc tactacttca aaccaatcca atttcccatg cgttttagcc acttgcttta
     6361 ttgcattatc aattgctttt tcaattcctt cctgcgaggt cccaattaac tccacgattt
     6421 ggtaaacttt atctgtcact tttctttccc cctctattaa tttgttgctc agcttttgct
     6481 attgccttgc gaatagccaa tgcttcttcg gactgcgctg gcgccaactg gagtagtcgt
     6541 tgccagtaat taatcgcagc ttcataatca tgacttaagt aagcatgcat cgcaagcatg
     6601 gctaaagcat ccggttgcct ggggttattt tttaataagg tatgaaaaat ttctaaaata
     6661 tcaggattga actgttgatt actcaattgc cataaactgt gggcataatt gactgcaaat
     6721 tgctcgtctt caggtttaag ctggtgggct tttgcaaaag cgagggaagc attttggtta
     6781 tccttttgac tgctgtataa tctgcctaac aaataccaac ccttggcact ttcaggctgc
     6841 gcatccaact tggccttcag cttgtcgatt aattcctgag ggcttttaat cgattttagc
     6901 atcgattgcg ctagctcctg agcctcattt cgatgtagat attcttgcca ccgagcaaaa
     6961 tctccccaaa aatagtaccc tgtaaaaacc attataaaaa taatgggggc gagcaacaaa
     7021 cttaccaaac gattgcgccg caaaggatag gtaataagag tgcatgcaaa aacggctaaa
     7081 acaaccaata aaataactaa ccaccattca ttcatgagaa tttcgcttaa aacatgtccg
     7141 ccaaaaaata ataaagccaa tcaataaaaa caggaatggg ccaaaccaca ataaaagagt
     7201 cacgctctta acaggcggtt taaatagtat aaaatcccca taacgtgcgg ttagataatc
     7261 cattatttca ctatcgcttt tgccctcttt cactaattca taaacctgaa aacgtaaatc
     7321 cttcgctaat tgagcatttg aatctgccaa atcctgattt tggcatacca aacaacgcaa
     7381 atcttttaaa agatggttaa attgtgcttc ttttcttgct gagtccaatg gatacatact
     7441 atttgcaaac gcaacggata ctaatagcag caataaaatc accttaaaac agaagtggaa
     7501 tactctcatt atgatttctc caaattcctc attaaaggta aaatatcagt taaccaggtt
     7561 ttctgattca ttatcccgac atgcctgtag cggactattc ctttcttatc aataacaaac
     7621 gtttcaggcg ccccatatac acctaaatca atagcgactt taccctctga atcctggcct
     7681 atgagtcgat aagggttccc ccattgtaca agccatgctc ttgcgtgttc tggattgtct
     7741 ttataattta aaccatagat aggtacgcct tctcttgcga gttgcaacat aaatacttgc
     7801 tcttcaacgc aagcctcgca ccaacttgcc cagacattaa gtaaaacgac ctgattatga
     7861 aaattcattg cactaaaagt agaatcggga ttttgcaact ctggtaaggc aaagtctggt
     7921 aatggctttc caattcgagc tgaaggcaaa tgatgaggat ccaaagataa acctcgccag
     7981 aggaaaaaac ataaaagaca aaaaatagcg acaggaataa cacgccatcc tatttttttc
     8041 atatcttgat ctcccgcact gacattaccg ttacttgcct ttttctgtaa taccttctat
     8101 ctgttaatgc caaaacgcct ccggctaaaa tcataaatcc gccagcccaa atccagcgga
     8161 ccaacggttt ataataaata cgtaccgacc aggcatcctc agacagtggt tcccccaaag
     8221 caatgtaaat atctcggaaa ggagtaacat caatcgcaga ctcagtcatt ggcatttgtc
     8281 caatggtata cactcttttt tcaggaaaaa ttttcctggt tttattctga tattgtattg
     8341 aaaactcagc ccttgtccct ttgtaatttg ggccattcaa cggagtctga ttaatgaatc
     8401 caatctgata atccagcaga ttcacttgct tgccaggaga catacgtaca tcatcttgta
     8461 cgccataagc tgaagaaaca gcaatgccaa tcactgttgc tgcgacacca caatgagcca
     8521 aaaccatccc ccaataagcc tgtccaactc ctgccacccc tctatccctg actttgtgat
     8581 acgccgcatt agcagtactc aacaaaaccc agaatgccaa aattaatccc aaaagggctg
     8641 taaggtgtat ttcagttgag gtacagaata gtaaaatgac aggcaatatc agactaagca
     8701 aaaaaatacg atttagcttt ctgaagacat ccctccaaca atcattctgc catttcaaac
     8761 ggacacctaa ccccattagc aacaataaag gaatcattaa tggaacaaat actgcattaa
     8821 aatagggcgc tccaacggat aatttaccta accctaaccc ttccaccagc aatggataaa
     8881 ccgtgcccat taataccgtc agcataataa ccactaaaaa cacattgttt aacaacaacg
     8941 cactttctcg ggataataag ccaggatgtt tattgacttg caaggtttgg gctctgaaaa
     9001 gaaacaatgt taaagaaccc ccaatgacga ataacaaaaa tcccagaata tataatcccc
     9061 gctgaggatc aaccgcaaag gcatgaactg aagttaaaac tccagagcga acaagaaaag
     9121 taccaattaa acttaaagaa aaagcagcga ttgcaagcaa catggtccac gctttaaatt
     9181 gctggcgttg ctctgtaact gccaaagaat gcaatagtgc cgtaccaacc agccagggca
     9241 taaaagaagc gttttctaca ggatcccaaa accaccaacc accccagcct aattctctat
     9301 aagcccacca actgcctaaa gtaattcccg cggttaaaca acaccaggca gccagagtcc
     9361 agggtctggt ccatttagac caactggttt ctactttccc tgcccataaa gcggcaatag
     9421 caaaagcaaa agcgacacaa aaccctacat atcccatgta caacatagga ggatggaata
     9481 aaaagccagg atcttgcagc aatggattca gatcacggcc ctgtgtattc aatacttgaa
     9541 attggcgcaa gaaaggattg gaagtcatta acaaaaaaag aataaaacca atacttagcc
     9601 aacctaaaat aactaatacc cgagcgcgca tttccgtatc aaggcttgag ctaaaaaaac
     9661 aaaccaggat catccaaaaa ctaaggatag cgacccataa cagcatggaa ccttcatgcc
     9721 caccccacac agcacaaaat ttataaaacc atgggagcaa taagctggaa ttattcagca
     9781 catagatcac cgaaaaatca tccatcaaaa aacaggccgt gagacatagg taagcacatg
     9841 ccacaaataa aaattgcagc acgacataat tcctggagga atcaatccag tctttccgat
     9901 tgaattgcaa acccgccaat gggatcaaag ctaacatcaa agaagccact aaagccaaaa
     9961 ccaaaaaaaa cagccctaat tccgctatca ttgtttcacc ttatcagcta aagcagactt
    10021 tacttgaggt ggcatataat tggcatcgtg ttttgccaaa acttgtgtag catgaaaatg
    10081 atgattatca gttacttctc cttccgcaac tattccttgc ccttctcgaa ataaatcggg
    10141 aagtatccct gaataggtaa caacaatcgt gttttcaaaa tctgtaattt taaactggac
    10201 atccaaacct tttttggcac gcactatgct gtttgcttcc accatacctc caactcggat
    10261 gtggtgtttt gctggtgctt ctcccgcaac aatctgcgtg ggggtgtaaa ataaactgat
    10321 attttgtctt aaggcatata gaaccaaggc agccgctgca gataaaacag acagggcaaa
    10381 cagtaagata aataatttgc gtcgccgtac aggggtcatt taacctatct cttaaaccaa
    10441 cgttgtaatt ttttgcgggt acgagttctt tgccacttta ttcctaaaat attcatgccc
    10501 aaaaccacgc aaaccaaacc ataagcaggc caaacataga gagaataacc gcccattgcc
    10561 aaccattcaa aaaactgatt catcagactc ttcctcaaat tgaatcttaa cccatgattg
    10621 ctttctctcc ctgaataaga cttcattacg tgctttttcc aaaatgatcc ataaggaata
    10681 caagaaaaaa cccagcaaag tgattaacaa tggatacaac atactgagag caattttggg
    10741 tttggcaaac acagataaag ttgcaccttg gtgtaaagta ttccaccaat aaactgaata
    10801 atgaattatt ggtaaatcaa ttaaacccac caaagctaaa attgctatta ttttatctcc
    10861 atcttcttta ttttttaccg cttgataggt agccagaatt gccagataga gcaacaaaag
    10921 tattaattct gaggtcaggc gggcatccca tacccaccag gcaccccaca tgggtttacc
    10981 ccaaatgctc cctgtaatta aagcaagaaa tgccatgcag gcacctaatt gcgcgacctt
    11041 atgaatcaaa agccctgcca ttttgatacg ccacaccaac aataaaatgg ccagaaaccc
    11101 catccaggca tacaatgcca ttgataaaaa agcgctgggt acatgaacaa aaataattcg
    11161 gtatgcatcc ccttgctgat aatctggtgg agcaaatacc aatccccaaa ccataccaat
    11221 ggccatggta gtcaaagcac tgactgccaa ccagggaatg agacgtcccg cgtagttata
    11281 aaaatttttt ggcgatgcca actgatacaa tatcttccac ataggaaaca ataaaatcaa
    11341 aaagaaggat tctaccacgc atcaaaacaa tcacaaaatt tagttccaaa cttagggtct
    11401 gttgacattt cgctcgcttg agtcgaaaag ccttgatttt cgagcaccgt agcggagcat
    11461 acatagaagt atgtgagtaa cggagcacag acaatcaagg cttttcggcc aagatagtag
    11521 aaatgtcaac agaccctagt ctatatgact aatacgtatc actgccgcaa tcgcataagg
    11581 tagaaaacca acagccaata gagacatcgc caataataaa gccagatagc cgctgaccgg
    11641 caaaccttgc atcgcaacgc tcaaagtacc actaccaaat atcagtaaag gtaaagttaa
    11701 cggtaataga atcaatgcca tcaaagcccc acgctgattt atccctatac caaaagctgc
    11761 tgcaagcgca cataaaaata atatggcagg cgtcccgcaa ataaggctca ataacaatat
    11821 acatgtttcc caaacattga atgaaaataa tattgcaatc aaaggagata ataataataa
    11881 cggagtcacg gatatcaacc agtgtgccat aactttggct gtcacaagga gatttaatgg
    11941 ttgccctgaa actagccact gttcaaggac tccttgttca taatcttgtt gaaacaaccg
    12001 ttctaacgaa agaagcatgg ataacaaaat ggccatccac actaatccgg gagcaaacat
    12061 acgcatcaac attaattctg gtcttagagt aagagggaaa ataaataaca acattaaaaa
    12121 aaataaacac gaattaacta aatatctgat ttgccgcacc tggattaata tttcacgcct
    12181 gaactgtttt ccgaataatt gaaatgggga attcatagat gatactcctc ataatccgcc
    12241 ttgtttaaag gcaaatcctg atgagaggtc aataatacag caccaccttt tgccctgtgc
    12301 tcctggattt ttgtgataat tgtaagcaat gataactcat caagagcaac caaaggctca
    12361 tcaagcaacc ataattttgc ttttgacatc cataatctta ataaagcaac ttgccttttt
    12421 tgtcctgagg atagtaatcc acatggataa tcaataaggt gttctaaact gaataatctg
    12481 cataattctg taatgcccac tgcaccagga gaaaaatgta tgtcatataa acaattttct
    12541 cttagcgtaa gatagggatt gatccctgat ctatgcccca caaaacataa ttgcttttga
    12601 taggtgcata gatctttttt tatagattgc ctttcgaaga gaatttctcc cttttcagga
    12661 ttaagcaaac ctgcgattaa tttaagtaaa gtcgtttttc ctgctccatt ggaacctttt
    12721 aaatgcaata agcctccagc aggaagatga aaagaaattt gttgcaacaa tggctggtca
    12781 tgatagtcaa aatcaagttc aatcacatca agcataaaac actttatttt tagatacact
    12841 cggctaacta taacaggaca taagaaaaat ccccaatgcc ttcgtgaaaa gttatttaat
    12901 ttttgcttgc gaagtgagtg gatgatcgcc ataatctgct ttattttttg caaaggtata
    12961 tgatggaatt ccttagtgaa tatggcatgt ttctcttaaa atgcatcact ctcgtcatcg
    13021 cattactcat tcttctggca ggtatatttt ctatgggtcg caaaaccaaa cccaaactgg
    13081 aaatcacctc tcttaatgaa gaatatcaac acctgaatgc cttaatgaat aaagagattt
    13141 taggaaaaaa acctggaaaa aagaaaaaag ataaaaccaa gcgcccggtt ctttatgtca
    13201 ttgatttcag cggtgatatc aaagcaaccc aagttgaaca actaagagat gaagtcacat
    13261 cggtattaag tatagcaaaa cctgaggatg aggttttagt aaggttggaa agccctggag
    13321 gggccgtcaa tggttatgga ttggctgcgt cccagttaca gcgaatccgt gataaaaaaa
    13381 ttcctttgac tgtcagcata gacaaaatgg cagccagcgg cggatattta atggcctgcg
    13441 tagcaaataa aataattgct gccccattcg caataatcgg ctctataggt gtagttgctc
    13501 aaattcctaa ttttcatcgc tggttaaaga aaaacaacat tgatgttgag ctattaactg
    13561 caggagaata caaacgcacc ctaacacttt ttgcggaaaa tactgaaaaa ggtagaaaaa
    13621 agtttcagga agatttggaa aaaattcata ccgcattcag agagtatgtt cttaaaaata
    13681 gaagtcaact cgatatagat aaggttgcaa ctggtgaaca ttggatagca aaagatgctt
    13741 ttgatctccg gctcgttgat aagttagcta ctagcgatga atatttaata gaaaaaatgg
    13801 cggagtttaa tgcattcaaa ttaactgtcc ttgctaaatt gccaattata agcaaggtgc
    13861 ttaaaccggc aatgagactc attcatccgt ggatttaaaa attagtactg aaacagcttg
    13921 ggaacgctat tgaaattcaa taccgttaaa tcaggcagca ttccatagga aacgagatca
    13981 attttatcat gggcttaaag attaatgatg gtagcggaat ttaaggaatt aatgagcaaa
    14041 aactttcaaa atctgccatt aaccaaaatt tgaatctcgg tatataatta aagaataaca
    14101 atttttctga ggagtaagac atgagtgatt tcaaatcgaa gttaccagat cttaatgaat
    14161 taacatcaat gaccgcgaaa ttatttaaag ggattaaaac cagtgtcagt gaaataattc
    14221 aggactataa gcaaaaaaga gcacagcctg ttagtaccga agagaaaacc acagaaaata
    14281 agccagctga aagcaaggcc gaaccaaaaa cagaaagcaa gaccactgaa aacaaaactg
    14341 agccaaaaac agaaagcaaa cctgaatcaa atacagaaga aacacaggaa aaagagaaat
    14401 aatcctgtgc tagcaaataa gtgatacggg gtgagctgta tctatttatc gttccattct
    14461 ttctggtcga gttaagccat atttgcgcat tttttcaact aaagttgtcc ttctcatgtt
    14521 gagataactg gccgcatgag caacaaccca atctgactca tctaaggcct ggctaatcaa
    14581 ggcaagttct gttttaacca aatgctcttt taaatcaatg ccctctgcat tagctactga
    14641 ctcctgcttt attatttgca acaaagtttc acgttccgcc cccgatgcct ctggacaaga
    14701 tggtttattt tcaccttgaa accgatgagg taaatcactc ttgccaataa taccattagg
    14761 gtaaagaata gataatctct ccaccaaatt agctaactcc ctcacgttac ctggccaact
    14821 gtattcgcat aaagcatcca tagcatcggg cattaatcga acggtaggtc tgccctctcc
    14881 ttcaattcga gaaaccagct cattaaaaag cagtgataaa tcactacttc tattacgtag
    14941 tggaggcatt tcaatgggaa atacattcaa tctgtaaaac aaatcttctc taaatttccc
    15001 ttcttgaata gcaatttcta aatttctatg tgtagcagca atgatacgaa cattgacttc
    15061 gatgctttta tttgaaccta ctctttcaaa gcaacgttct tgtaaaactc ttaataactt
    15121 aacctgcata gctaatggca tatctcctat ttcatccagg aatagagttc ctccattggc
    15181 caactcaaaa cgtccttgcc ttgatgtaat cgctcctgta aatgcacctt tttcatgacc
    15241 aaataattca ctctccaaca attcccccgg gattgctcca caattgatag ggacaaatgg
    15301 tttatttgct cgggatgaaa gagaatgtat attgcgagca acgacttcct tacctgtacc
    15361 tgactcacct aaaatcaaca cactggcttc agtatcggct acttgctcaa ttagtttgcg
    15421 tacttgacga ataccttcac tattcccaac caagcttcta aataaaggag tcttatgggt
    15481 acctgcagtg ataaatttaa ctgcttcttt agctatttga cacctatgga gcgatttaag
    15541 catttttgca tagctgaatg gaaatgataa acaatcaacg atatttcgat ccgcgcattc
    15601 agatgtagaa agcacagcat caataaccaa tattggagtt tttggaaacc ttttaactaa
    15661 tgtatccaat tcatccatca ttttttcaaa agaagcgcac gctccaagaa ctatcacact
    15721 tggattagga ttaacactca attgccaatt gccatattga gtgacctcag agaattggcc
    15781 tataaaatca aaaatagtcc gaagcttgtc acaacgttcc ttatcatcat cgataatata
    15841 aatcctgtca ttgctcctca taaacctatc cttaagtttg agtcatttaa tttaaagtat
    15901 cggacacata atgaataact gtcaaataat tgtcacaatt tattttaacc gtcataaaat
    15961 ctatcaaatt aggccgtatc gctataatta aaaaaatact aacctatccg gatgctaatt
    16021 aaaaaatcat agtcatcacc agactaagta tttgatttat ctgtaacaaa taatcatctt
    16081 aaacgcatta cactgattac caaaacataa ttcctgactt cctatggtct tcgtagcgct
    16141 cacttgagcc tgctttacga aatccttggc ttgcatatat cccatacgat tacagaacga
    16201 attggcagct tgtttaccac agcctttatt gcgctccata caccaatcca ctcggtaatc
    16261 attataacgg ggtacggcaa attttttctc tcgataatgg tagggtttgg gaggagtatg
    16321 tgataaacca atagcacaag ctattgtcat aaaaccatta caacgccatc ctttgcattg
    16381 agccttgcta cccaagtaat tagttaaacc gacgttatag gcaatgacat tttgagtgga
    16441 gtaatcataa cccagcattt ggcaataacg attcgctacc tcctttccgc actctttccc
    16501 atctaaagta cagtaatcaa gtcgttgccc taaataagta ggatgccaaa aatttctaaa
    16561 tgccaaattc tgcttacttg aagctgttaa ctgaccgcaa aaagaaaaaa ataaaagtat
    16621 aaaaaccagg aaatttctaa aattgctcat tataacaatc cattaccaag tattagcaca
    16681 tgttagctta ggatgactgg gaggcacaag agagatttat tcttctttta ctcgtactgt
    16741 aatgaacttt tgtcttattg cattttctag atacgactat gatttaaaag ctgaccaaag
    16801 acaaaacaac tatatcaaga taatttttca aaggctataa acaatgttat aaatagcata
    16861 tgaacagaac ttatcccctg gaatcaaggc aacaaatgtt tttaataaga aagtttggcc
    16921 aaattgaatg acagaattaa aaacactttg taacaaagag cataggggct taagcaaatt
    16981 ctaatgaaac aagcttttca atcatcactt taaaagccca tatcaaaggc tagtcgttta
    17041 tgaaaaaata tttaactttt atagtgtcag gcttagcctt acttgcaata atatggttca
    17101 ttcgccactt atggataatt ggaactgtca aggttgtgcg tcctgtttat ggaactgcag
    17161 ttcaagctgt ttatgccaca gggacagtcg aaccaaccgt catgatgcca attgcctcac
    17221 aaaacacagc ccgccttgtc aagttgaatg cagatgaagg ctcggaagta gtaaagggac
    17281 agatattagc cagcctggaa gatgaacacc tgcaacataa cttaaaagag ctgaaagaac
    17341 atgaaacatt agcaaaaata agatacgagc ggaatgctca attagttaaa aaaggagtca
    17401 tctccaaaga tgagtatgat cgcaccttgt ccgaatggca agcagccagt gccgccgtac
    17461 gcagaataga agctcaaatc agttatttac aactgatagc ccctgcagat ggccgtatca
    17521 ttaaacgtga cggtgaagtt ggacaactaa tcccggcaaa tcaacctgta ttttggcttt
    17581 cttgctgtgc tcccctaaga gtttctgctg aagtcgatga ggaagacatt gtattggtca
    17641 gagtcgggca aaaagtgttg atcagatcag atgcttttcc aaatcaaatc ttttatggcc
    17701 gtgtcaaaag catcacccct aaaggcgatc caatagccag aagttacaga gttcgcatta
    17761 atttttcaga aaataatcct ttattgattg gtatgacagc tgaaaccaat attattgttc
    17821 atgaaaagaa aaatgcctta ttgcttcctg taagtagtat ttctgaaaac aaagtttgga
    17881 ttgtacagga tgatgggaag ctctctcaaa atacagtgac tattggcgca agaggattaa
    17941 aacaagccga gattttaaaa ggtgtcaata ttaacgacct ggttgtctta caacctgata
    18001 ataagctaaa aaatgggcaa aaagtgcgtt tcattcttgt aaaacaggaa gaataatgaa
    18061 gctattttta aagatcgctt taaaacattt gcttgcacgc aaacgacaaa gcctggtttc
    18121 tttgatggga attgtattgg gagtagcctt ctttttaaca atttcgtcac tgatgcaggg
    18181 gtctgaaaaa gatttcatca aacgcttggt ggataattca ccgcatatca cgatcataga
    18241 tgaatatcga aaccctcgta tacaacccat ccaacagctg cacacacatg gcgctattga
    18301 aattcgcagt gttaaaccat taacagaacc ccgagggatt agagggtatg aacaaacaat
    18361 aaataattta tcgcttacca tcccgggaat tgttatttct cctgttctaa ttggacaagc
    18421 cttaattagt tatgcaggga aagattattc ggtcactttg aatggtatga tccctgaaga
    18481 gataaaaaaa gtttctacta tcgaaaatta tatgacttca ggttcgattg atgatctcat
    18541 agtcaatccc gatggtattg tcttgggaag cgagctcttg cgtaaattat ccctgacaaa
    18601 agatgataac attactgtga cagcgcccaa tggtcaaata cgcactttca aaattcttgg
    18661 cgtatttcgc actggccgtt cagattttga taccaatcaa gcgtttatta gtattaaacg
    18721 cgctcaagca ttattaaacc gacctaaccg ctccaataac atcattataa aactgcctaa
    18781 cccctatcag gcctatgaca ttgccgctca aattgaaaaa cagataggct ataaaactat
    18841 ttcctggcag gaaaaatcgg aagatttgct caatacacta gtcattcgaa atatcattat
    18901 gtatagcgtg gttagcgctg ttttaattgt cgctgcattt ggaatttata atgttatttc
    18961 gactgtagta atggaaaaac acagagacat tgccatactt aaatcaatcg gtttccaaaa
    19021 acatgatatt caatttattt ttatcataga aggttttctt ctaggactgg ttggatgttt
    19081 gttaggactg cctttgggaa gtggactgat gtacagtctc atgcaagtcc aattcaagcc
    19141 gccaggaagc tctgaattga ttaatatgcc actcgattgg agttatttac aatttgtcat
    19201 cgccacagct tttgcgatgt ccgcctcaat catagccgcc gttcttcctg ctcataaggc
    19261 agcgcttgtt catcctgttg atatcctgcg aggaggggga atgtgaacaa gattgtcata
    19321 aaaacaaaag agctttcgcg tcaactgcca ggtgaagttc ccgtcacttt agtcaaggac
    19381 ataaatctcg aagtcagaga aggtgaattt ttagtaattt ctggcccctc aggttctggc
    19441 aaatcgtcac tgctttattt gctaggcttg cttgatagac cctctcaagg aacaatttgg
    19501 ctgaataatg aagatgtttc aacttatgag gaagaaaaaa tggcctggtt gcgcttgact
    19561 caaattggtt atgtctttca attccatttt ctattacctg agtttacagc cctggagaat
    19621 gtgatgttgc ctatgcaacg cctaggcaaa ctaagtcttg gggaaatgaa aaaccgagct
    19681 accaagcttt taaccagcct agggttagag gagcaattat caaaactgcc taaacagctg
    19741 tcaggagggc aaagccagcg cgttgctatt gcacgagctc tggccaatga tcccatattg
    19801 ctcttggcag acgagccaac tggaaatctt gataccgttt cgagtaccaa tgtgcaaaat
    19861 atactaaaag aacttgctca tgaatacaga agatcggtaa tcatcgtaac gcacgatatg
    19921 aattttgctg ctacagcgga cagagtgatt cacattgtcg atggcaaaat gcaagaataa
    19981 cctcacttct tgctagtcca tgcttacttc atcagtttca tcctgatcta ttcttcagga
    20041 ctaacgtaaa accccaaggt cgaggcaaaa aatgtttgta atgagtgagt ttaaataata
    20101 aactaaatga acgaattaaa aacatttttt aacaaagaga ttgggatttt gcgtaggtcc
    20161 tgttcttttt aaatcggatt aggactatct ataaaccaat gttccaagtc aaaatagttt
    20221 gcaagataaa caccgaggga ttgaatgccg aaacgttccg tcgcatgatg accacaagaa
    20281 aaatattgta tacccaattc catagcctga tagaacgttc gttcagaaac ttcaccactt
    20341 aaataagcat caacgccaag ttggtaagct tcttctataa agtcttgtgc tgctccagtg
    20401 caccatgcta tggagtgaat cattttttca tttcccgcaa tatgcacagg ataacggcca
    20461 agtttgtgct ctaaaaaact ggaaaactgt tcgctgttca ttgcctttga taattttcca
    20521 gaccaaagga gattggcagt attgttgacc ttatgcatct catgggaccc tattaacaac
    20581 aaattcgcca ggctggcatt attccctagc tcgggatgac aatccaaagg caaatgatag
    20641 gcaaataaat tcatgttatg gccaagtagc ttagcaatcc ttcgcctttt cattccggaa
    20701 ataacaggat tttcaccttt ccagaaataa ccatggtgaa ctaacagtgc atcagcttgc
    20761 tgttcaatag cttgggaaat cacgtcctct gatgcactga ctgccgtaca tatacgctta
    20821 atcttgtctt tgccttcaac ttgaattcca ttaggtgcat aatcctggta ttgagaacaa
    20881 ttcaagaaat ccagtaaaaa aaggcttaat tcatctcttg taatcacctg tcagaaactc
    20941 tcttaatgtc cgctcccaat agagataact tttcttctat tcgctcatag cctctatcaa
    21001 catgataaat ccgctcaact actgtttccc cttcagcaac caggcccgcg agaattaaac
    21061 tggctgatgc ccttaaatca gtagccatta ccggtgcgcc agttaatttt tcaacaccat
    21121 gaacgatggc tgtatttcca ttaagttgga tattagcccc cattctctgt aactcttgaa
    21181 catgcataaa tcggttttca aaaatagttt ctataatagt tgaagaacct tctgctacgg
    21241 aattcattgc cataaattga gcttgcatat ccgttgcaaa tgcaggataa ggggcggttg
    21301 aaatattaac tgcctgaggc cgcttgttat gcatatttaa actgacccaa tcctcaccaa
    21361 tggttaattc cgcgcctgcc tcttcgaatt tacataattg agataataaa gtatcaggac
    21421 gtactttctt aacagtcact tgaccacgag taagcgctcc agctgccaaa taagtgcccg
    21481 cttctattct atcggacatg accgaataag tcccaccgtt tagtgattcc acaccttcta
    21541 cttcaatagt agaagtaccc gcgcctcgaa ttttcgctcc catttgaatc agaaagttag
    21601 ccaagtctac tacttctggt tcgcgcgcag catttttaat cgtagtaatg ccttctgcca
    21661 acacagcagc cattaacaca ttttcggttc ctgtgacagt aactgtgtca aacatcaagc
    21721 gtttaccttg cagacatccc tttttgcaac gagcattgat gtagccattt ttaaccgtaa
    21781 tgtctgcccc catggctctt aatgctttta aatgcaggtc aactggtctt gttccaatgg
    21841 cgcatccacc gggcagagaa acatctgcct taccgaagcg cgccagcatt gggcctaata
    21901 ccaaaatgga ggcacgcatt gttttgacca gatcgtaagg agcaacaaac tcattgactt
    21961 gactggagtc aacttgaaca ttcatctttt catcaacaat taaatgagct cccaattgtc
    22021 ctaacagttc catcatggta gtgatgtctt taagatgagg cacattagat attgtgacgt
    22081 gatcactggc taatagactg gcagccatga ttggaagagc agcgtttttt gctcccgaaa
    22141 tgaccacttc accatgtaat gctttaccac cattaatcaa taatttatcc attttgcttc
    22201 ccccattctt cttttgtcca tgtcttcata ctgatagcat gaagccttcc ggtggtaata
    22261 aagtctttaa gctgggcata aacccattgc tgtcttgcta ctttagactt cttaagaaat
    22321 atatcggtca ctacagttac ctgatattga taaccatcgc cttctacctt gacataaaat
    22381 acatcgtcaa tttcattaag tctttgttca atttcttcat tacttaacat aaacttagct
    22441 cactaattaa aataaataca aattttttat acttttattg ataaaaaata atggacatga
    22501 ctgaatcatc atcaaaaaaa tccgtgttta caccatcatt ctctaaagat ttagactgtt
    22561 tcaccaacca caacattacc tcaaaactca tgggaattta tcaattcagg aggacaacta
    22621 gagtctgtta atttttctct ggcttaaaac ccaaaacttt gatttttcag caccgtagca
    22681 gaaatataaa accattatta agcaacggaa cacagaaaac caacgcttta aagccaagac
    22741 agtagaaatg tcgatagatt ctagcgctct aaagggataa gctctatgga ataataaata
    22801 tttctgactc agaattattt caagatcttt gtaaaatagt cttcactcca cagaattcag
    22861 ccagggattg tgtttcagga gatattccta ttacatcaaa aaccttatta ttttgtttac
    22921 ataattttct agcttcaata attaaagcaa gtccagcact atcgcaatga gtcacctcgc
    22981 ttaaatcaag gcaaaatctc cctctggtat cttccattaa agccttatac agcttgtcac
    23041 ggaccgatac cactgatttg aatgtaagct cagcgccagg tttaaagtta ttaatttcca
    23101 tgctcaggaa gccttcttaa tctgtttttg ttgcatttcc ttaatcactt gactaatgct
    23161 ggaatgctgc agtgcctgtg caaattgcga tctaaaactt tgcaacaaac taacaccttc
    23221 aacgcttata tcataaattt tccatttccc attcttggaa acaagactat aacttaatgg
    23281 tatattttgc cctgcagaac gcacaatcac gctatttaca cgaataaacc gactatttaa
    23341 agaaccgcgt aaaggtaaaa actgcacagt ttcatcggaa tattgggcca atgggctaga
    23401 atatgtacga atcaccaagc gagtaaatgc ttttgagaat tcaacccttt ctgcttcagt
    23461 agctttactc caggcttgtc ttcccaagac tgaacgagac ataccagcaa cgtcaacgtt
    23521 gggtaaaaga ttctcctcga ctgctttgta aataatctca ggattacttt ttaagcgcgc
    23581 tttattttct ttgagaatag aaataatttg attagctgca cgttctaaca tagggatagg
    23641 agaggtctga gcaaccattg cttgagacaa cgtcatcaac acaactaata aaattgtttt
    23701 aaaaactctc atggttcacc tattttttta tattaaataa taattggcct ataagatttt
    23761 caaggataat tgcttcctga gtcttcgcaa tggtatcccc atcgcgtaaa tagggatgat
    23821 ctgtgtcacc gtcctcgtca aaaccgggaa cgatactaat ataattcgaa ccaagcaagc
    23881 cttcggtcaa aattcgtgca gaagcatcct cataaggaat ctttttatca ccattcaaag
    23941 tcatagtcac tcttgcattg agctcacctg gctgcaactc aattcgactg acctcaccaa
    24001 tacggacacc tgcaacggtc actggagctc gaacttttaa accaccgata tcagtaaaat
    24061 ctgcagtgac acgataagtt ttatcagaca taaaatcagt aatgttactc actttcatcg
    24121 ccattactaa taatgctagc agcccaagca acataaatat tcctacacta atgtctacat
    24181 aacgttgctt tttcatttac caatctccaa tcatcattgc agtcaacaag aaatcaagac
    24241 ctaatacagc aagcgatgag tatactacag ttttagttgt agcatgacta atgccttcag
    24301 ctgttggcac acactcaaaa ccttggtata cagaaatcca agtaaccaca aatgcgaaaa
    24361 ccaagctttt aataatcccg cttaagatat ctatacgaaa atttacagca gcttgcatat
    24421 tagaccaaaa actccctgca tccacgccta gccagtaaac accaataaaa taaccaccat
    24481 atatagctac tgcagaaaaa atcaaagcaa gtatcggtaa cgctataaat cctgccacaa
    24541 aacgcggata aaccactctt cctaatggat ccactcccat catatccata ctggataact
    24601 gctctgttgc cttcattaaa ccaatttcag ctgtaagggc tgacccggca cgccctgcaa
    24661 acaacaaagc gctaatcaca ggtcctaatt ctctggcgac gcttaaagcc aataattggc
    24721 ctaattgact tgaagcacca aatttttgta acgtattata accctgaagt cctacaacca
    24781 taccaataaa caaggctgaa accacaataa ttaaacaaga caatacaccg ataaaataaa
    24841 gctgataacg taataatggc cataacctta aaacattagg ctttctgaac aacatagaaa
    24901 ataaaaacag ccctgagcta ccaatacctt gtaaggcttg tacacctcga ttacccagac
    24961 gcgcgattgt atcaagcatt taataactcc tctgtatacg gttttgccgg ataatggaaa
    25021 ggcactaccc catccgcttc tccatgcata aactgtctta tctgaggctc aatagatcgc
    25081 tttaattcat ccggagtacc ttgacctata attttacccc ctgatattaa gtagatataa
    25141 tctgcaattg aacaagtttc ttcaacatca tgagatacga taatagtggt ggtatgcaat
    25201 aattgattca atcgttgtat taatctgacc aataccccca tggaaattgg atcttgaccg
    25261 gtaaaaggct catcatacat cattaataca ggatcaagag caatggttct ggctaatgca
    25321 actctcctgc acattccccc tgataattga gcgggcatta gatgggcggc tccacgcaaa
    25381 ccaaccgctt cgagcttcat taatacaatg tttcttatca tggacgcgtt taattcagta
    25441 tgttctctta atggaaaggc aacattttca aatactgaaa ggtgtgtaaa taaggcacta
    25501 ctttgaaaaa gcaatcccat gtttcgcctg gtttcataaa gtgatttgcg ggataattga
    25561 tgaatattct gaccattaac ataaatttgg ccactatcag gcctcaattg cgcaccaatt
    25621 aaccgcagta aagtagtctt tccagaacca ctgggtccca taatagcggt aatcttgcct
    25681 tgcttaacca ccatatcaat gccatcaaaa atgcagcgat catcaaggct aaagaataga
    25741 ttttttattt caacccaatt atcaggcatg ctttattatc cacttattat tttaacgggt
    25801 aatgatgtca gtttg
//
LOCUS       DAXLDT010000033        25646 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXLDT010000033 DAXLDT010000000
VERSION     DAXLDT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25646
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.033"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(69..527)
                     /locus_tag="ACF2SP_001073"
     CDS             complement(69..527)
                     /locus_tag="ACF2SP_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFD2358867.1"
                     /translation="MTHDAFLDDLILSIVQTESIAEQSDLQNSLRKRGYEIPQATLSR
                     RLKKMKIAKVSGYYKIIDYNQPGLPLVLNVQISDFGLIILQTHPGNANSLAYYIDRKY
                     VSFSIKDSSNSGILGTIAGDDTVLLIIKSKSDQQKVLDILMKEFPYLFAS"
     gene            658..1392
                     /locus_tag="ACF2SP_001074"
     CDS             658..1392
                     /locus_tag="ACF2SP_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD2358868.1"
                     /translation="MRKVFSLLLISSIIVISSCSEQKNQNTLRFSTSAEYPPFEYIEN
                     GEIKGFDIDLAKLIAKELGKEAVFDNMQFSAVLPAVNSGQDDVAIATITITEERKKNF
                     DFSKPYYFEGMAAVFDTSQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPKAEITALDN
                     NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLKKGSPLRDE
                     INSALENLKAKGEIQKLESIWIKGSI"
     gene            1389..2036
                     /locus_tag="ACF2SP_001075"
     CDS             1389..2036
                     /locus_tag="ACF2SP_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFD2358869.1"
                     /translation="MTEIVQNLFFIGKGIVLTMQLLTGGMLIGVLLGTLLSICRHQGV
                     AGFVINRFISILRGTPLILQLSFIYFAVPVLLGMRPSILVAGIVAFGLNSSAYFAEIL
                     RSGIESLPKGQFEAALTLQIPTFYLWKDIILPQVIKNIFPAMINEMIALLKETALIST
                     IGGMDLMRQAQTLAAQQFTYFMPLCITGFYYYSLVLLLEALGKKIEKRGHYAASH"
     gene            2020..2688
                     /locus_tag="ACF2SP_001076"
     CDS             2020..2688
                     /locus_tag="ACF2SP_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFD2358870.1"
                     /translation="MLQVINASKKIGDVQVLNEINLSIQANSIVGLVGPSGSGKTTLL
                     RCIQQLESIDSGSIQCEGKSGFMFQDFQLFPHMTVLNNLIYAPSLHDKTADHIANAWA
                     LLAMLGLSDKAHSYPQGLSGGQKQRVALARSLMMKPDLLLCDEPTSGLDMATTNDVIA
                     LLNTVKTLKITMLIASHDLFFLSNIAERLLVVRQGQLIADFKTQEVSDPILYLQQFYQ
                     GEIL"
     gene            2685..3902
                     /locus_tag="ACF2SP_001077"
     CDS             2685..3902
                     /locus_tag="ACF2SP_001077"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFD2358871.1"
                     /translation="MKKVIKKIALAYSGGLDTSIMIPWLKEHYEHAEVIAVICDLGQQ
                     EDLDAIKNKALKSGASKAYVVDVKNEFAIQYLWPLVKSGALYEDQYILGTISRPLIAQ
                     KLVEIALTEEVNAVAHGATGKGNDQVRFEYSIKALAPQLEIIAPWRTWDIKSRQEAIV
                     YAKAHGIEVPVTPKAPYSRDHNIWYISHEGGVLEDPSQEMPDDVLLMTAPVSQTPDEE
                     EVVVLDFKKGVPVALNGQELSPVDLLNSLNQKAGQHGIGVADIVENRLVGMKIRGIYE
                     APAAAVLYKAHKLLESLCLTRSTLHLKQSLQQTYANLVYEGRWFSQTKQALDAFIDVT
                     QQHVTGCVKLKLFKGNIIPAGMHSPYSLHHPELATFEEDNVYNQKDAEGFINLFSLSA
                     KIYSQVHQGGNYD"
     gene            3895..5130
                     /gene="argH"
                     /locus_tag="ACF2SP_001078"
     CDS             3895..5130
                     /gene="argH"
                     /locus_tag="ACF2SP_001078"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFD2358872.1"
                     /translation="MTNKTWGGRFKKSLDSSVNQFNASLSFDHVLFDQDINGSQVHVK
                     QLAKQKILTEAECQGIYSALEEIRTEIKQGQYSFNERDEEDIHMFIEQLLIQKIGDLG
                     KKLHTGRSRNDQVALDLRLYTRDKGCLINELLTRLIDCLDDLTSKHQQDLMPGYTHLQ
                     QAQPVALGAYFNAYQCMFGRDKSRLDDWFKRMNYSPLGAGALAGSTLPLDREWVAESL
                     GFAGIIPNTLDAVSDRDFVIELCSVAAMIMMHLSRLCEDLILWSTQEFNFVILDDAFA
                     TGSSLMPNKKNPDVPELIRGKSGRVYGHLMAILTVMKGLPLAYNKDMQEDKEGLFDTI
                     NTIIACLQMITPFLQSLTFNTLLMKTKAQSGYLDATAILESLVMKGMPFRNAHHQVGA
                     WIAEAIEKQCSLNELLKGG"
     gene            5133..6248
                     /gene="argF"
                     /locus_tag="ACF2SP_001079"
     CDS             5133..6248
                     /gene="argF"
                     /locus_tag="ACF2SP_001079"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFD2358873.1"
                     /translation="MSFSTSTNLTPIEMQDDDLEKIQSQNNPSAINEVKTNTFVINLQ
                     QAIFGLETGSEAVDFDSHKNTGFPFPAGEYKPRHFLTGTELAREEIQFVLSQARSLKQ
                     NPGLYNQALANKSLVMIFEKPSFRTRLSFALAIQSMGGIVVESIANSRKQETPADMAR
                     VLNGYADFVMVRTHEDSFLQEMAEYSKIPIINGLSALHHPCQTLADLLSLEEQFGALE
                     GLTLTYIGDGNNILHSLMLLAPQLGVTVHYCCPKSNQPNEAIIKEAQSKTGKQMIYCF
                     DKPEEAVKQADAVYTDVWTSMGFDNPEAVKIFKGFQVNETLMSHAKPDSVFMHCMPME
                     RGKEVSATLPDSASSIIFRQSENRMHVQKALMLFLDQ"
     gene            complement(6337..7812)
                     /locus_tag="ACF2SP_001080"
     CDS             complement(6337..7812)
                     /locus_tag="ACF2SP_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFD2358874.1"
                     /translation="MNITRLLTFFTSFFLLCPIYASVDSYFSHIKKDSSALYAFFKTM
                     PKGGELHYHLAGGAYPETMLALAANGNYCLDKHSFAVSKDSSHCEGVNVKEVFNNPEL
                     YSNIIKDWSMKDFIPGKESGHDHFFNGFVKYMPIVFDYRPQLLADIVQRAAQQKEQYM
                     EIMIIPDNAHSLTYGDLIKDIPSYDQKRHLLLANKDFQSNIKNTVLESDRILAKASQE
                     LGCKDNPSSDSCLVKIKFIYYVLREQPVDNVFAQALNAFEAVSKSKGNLIGVNLVQPE
                     DGIISLRDYHKHMSIFEYLHRIYPNVPITLHAGELSPQAVTPENLSNHIRDALLIGHA
                     QRIGHGVDIGYENNAEDTLKYMANHHIPVEINLISNLKILNISGRNHPLNYYLTHNVP
                     VVLSTDDEGVLRTDLTQQYVEAAKEHGLNYQQLKQINRNTLTYSFLPGKSIWANADKA
                     ELIPDCHDLGSKSCQKFIEHNEKAKLQWSLEQQLIAFEKNY"
     gene            7987..9102
                     /locus_tag="ACF2SP_001081"
     CDS             7987..9102
                     /locus_tag="ACF2SP_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD2358875.1"
                     /translation="MPYFYTRSAPLFFVFVIALIFINFSYAHEFQTDSKNKHKVDVHI
                     GIYAPFSSHSAFIGRNILAAMEMGSERLQSSDIHYSFYTLDQLPKNKNAGLVLQKFID
                     THHINVLVTGGKETGLIAAPIAKKNNLIHFSLTNNTKVADGRNNFIVWSPVYDQAKVL
                     VNELKRQQVHHLGIITANQPSSTALTHSVIKQLQADSHIDVAASEFFEPGNKDFSRLT
                     SRIKRKNADMYLVMASPEDIKLIQEQMEADKQHVPITSIVERATPEVVKVFEGQWYVD
                     SHEMQPDFINEFKQNYLNYPVTEAGYAFDLFQLLNKSIVMAIRTQGSPTRQEVVHQLH
                     DIANSTGVMGTMVLDTKGSLFTKSEVKTVKNGEIHTV"
     gene            complement(9175..10512)
                     /locus_tag="ACF2SP_001082"
     CDS             complement(9175..10512)
                     /locus_tag="ACF2SP_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFD2358876.1"
                     /translation="MVIKKLFSSTLALVYALTLMLPLTAFSAEETNSNYSNTKRIPLE
                     DVQRFSNAIGEIKKYYVKPVDDKELFDNAIRGMLTGLDPHSSYLNEEEFKELQTSTSG
                     EFGGLGIEVTMEEGVVKVITPLVDTPAFKAGIKSGDYIIKLGKESVQGLSLKDAVNLM
                     RGKPGTTIELTILRKGVNKPLTFDLIREVIQIKSVKSKMLSEGYGYIRLTQFQALTGK
                     DMIKAIEQLKQQAGGKLKGLVLDLRNNPGGLLDSAIQVSDAFLGNDKAGKQEMIVYTE
                     GRLPGSKFTALANPGDVLDNAPIVVLINNGSASASEIVAGALKDNKRAIILGTKSFGK
                     GSVQTVLPLDGKTGIKLTTALYYTPSGVSIQAKGIIPDIIVEEVDVPKNAVKKDTLAG
                     FTEADLNGHLINKDNSENTESKNNQIQTGDLIHEDYQLYAALTVLEGMALANR"
     gene            complement(10593..11735)
                     /locus_tag="ACF2SP_001083"
     CDS             complement(10593..11735)
                     /locus_tag="ACF2SP_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFD2358877.1"
                     /translation="MHMPSLSNSLSGASLGLALMVILFFCNGLEAKAAPNSITQAQTK
                     LKQLDAKINKLKQVLANAQDKRTVLNQELGSTEKQIGEGIRKLRIIQANMSAKEKSIA
                     ELQGKINHLNKRLQAQQEMLANHVRARYQMGEYQPLKWLINQDEPFKINRILTYYQYI
                     IRSRQQLIDEIARTRQNLNDSKTKLGNELAENKQLQFKLSQNQKQLEQNKRYHSTLIQ
                     SIDNDIQNNQQSLKDFQKDRDNLSHLMKSLSQQSVIQSGKPFTGMRKKLPLPVQTTHR
                     SLRRMNQGVTFFADEGSIVTAVYPGKVVFSDWLKGYGLLLIIDHGQGFMTLYAHNQSL
                     FKRKGQIVQQNEQIASVGHTGGIKQNGLYFEIRQRGKAVNPLDWLS"
     gene            complement(11722..13266)
                     /gene="gpmI"
                     /locus_tag="ACF2SP_001084"
     CDS             complement(11722..13266)
                     /gene="gpmI"
                     /locus_tag="ACF2SP_001084"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFD2358878.1"
                     /translation="MSHNAPLVLMILDGWGYNENDRYNAIAKANTPQWDEWWQTCPHI
                     LLKASGLPVGLPDEQMGNSEVGHMHIGAGRVIQQDFTRINEAINNGKFAKNAVFHEVI
                     DQLKKTEKSLHIMGLLSPGGVHSHEQHLFALLALCNQKKFRSVHLHLFLDGRDTPPQS
                     ALDSLKCLNEELAKHPVATINSICGRYYAMDRDKRWERVEPVYNLLTQGKSERQFPDA
                     ETAIHFYYKNKISDEFVPPTLIGKEHSIQDGDAVLFFNFRADRARQLTSTFLDPLFKG
                     FERKTLPKLSCFVSMTQYDKNLFTTTAFPPVPLNNTLGEVLSSHGLSQLRIAETEKYA
                     HVTFFFNGGCESVFTNEERIMVPSPQVATYDLQPEMSAHELTKTLIAAINSRDYHVII
                     CNYANADMVGHTGNFEATVQAIECLDQCMQQVWQALKNNGGKLLITADHGNAEEMFSE
                     ATNQAHTAHTSEPVPFLYVGGGWHFTHSEGSLIDIAPSLLALLGITPPPEMTGRILLE
                     KNHAHA"
     gene            complement(13369..13665)
                     /locus_tag="ACF2SP_001085"
     CDS             complement(13369..13665)
                     /locus_tag="ACF2SP_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358879.1"
                     /translation="MSRDTRNLILKAIILFISLTALVNHAMAFNLLGIGPNPKNLPKE
                     NDVRASFNHCKSCDKSTTPPLIITTPVPIIYYPGCRPIQSCDAYGHCEQKEDCY"
     gene            complement(13742..15013)
                     /locus_tag="ACF2SP_001086"
     CDS             complement(13742..15013)
                     /locus_tag="ACF2SP_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HFD2358880.1"
                     /translation="MKKSIHRLVKPRLFAMSISLLAFSSIIYGESNGSFALSEHWSMG
                     QQIKLRFDINEVPQSGAVLHLKNGLALTAGDIISLGDLYGIVGKPISHGLTKLEKQTR
                     FKEVFNSFARNVIAVSEVEDLNSVIRAEMREVESGIAKGETAEAIYKRIGNEVGRQIN
                     CITGGGCNSLTWWLNPGRYLKLAIENYDHFTPNNLIAYKSGHHVALQQALKAKRTGKR
                     SDLEIAYAMDAFACHYLSDHFAAGHLRTPREELSAIITPTVLGALLSNYMHNEENKYG
                     IHVTNNLGEQWMVYGDYSYFNPFNQTNQQMLLKALQASADEIFEAYYSGVLPEKNQIE
                     DMIPHITKLNDENNMDIAPMFYWDKENKQLLRRTNLTNPYDRHWTNSWWGWSTLLLLK
                     TQYGITSTVQLSLSKYLSQYVPEELQDNPLV"
     gene            15363..16079
                     /locus_tag="ACF2SP_001087"
     CDS             15363..16079
                     /locus_tag="ACF2SP_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFD2358881.1"
                     /translation="MNHKLPQHIAVVMDGNGRWAESRGLPRVEGHKAGLDSVKKIINC
                     CLEKKISCLSLFAFSSENWSRPVTEVNFLMELFLEALRKEIDDLNQHGIRLKFTGDRE
                     PLSQVLQKQMYDAEALTRNNQQLILNVVVNYGGKWDIVTAAKKLIHSVLDGKLACDEI
                     NEAVFAQFLDTSGMPEPDLFIRTSGELRISNFFLWQLAYTELYFTDVHWPDFNEHEFE
                     LALTSFARRARRFGQISQSE"
     gene            16090..16887
                     /locus_tag="ACF2SP_001088"
     CDS             16090..16887
                     /locus_tag="ACF2SP_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFD2358882.1"
                     /translation="MFMQRLITTLILAPLVLLAIFYAPSWILWGIVLVILLLAGLECF
                     KLIPVDNLTVQACFLIFLLMCFWASGYFYPYWLSVGLLVWLLNGLAILTYPRSQNYWG
                     YPIVVAIASLILLPLFTQSLIQISFLQQGKELLVYLLFLIWASDIGAYLAGKQWGKHK
                     LIPKVSPGKSWEGVTGGVILAMIVATLGYYYFIPYSIYIWFGLALSTVVISIFGDLFI
                     SILKRRCQLKDTGNIIPGHGGILDRLDSLIAALPWFYFGLTYIPLGI"
     gene            16920..18272
                     /gene="rseP"
                     /locus_tag="ACF2SP_001089"
     CDS             16920..18272
                     /gene="rseP"
                     /locus_tag="ACF2SP_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFD2358883.1"
                     /translation="MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKI
                     LARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGP
                     LFNFIFAFVALWLVLVIGMHSLAPMIESVRPNSIAARAGLAPKQEILALNGVKINSWR
                     DFQYEIMPLIGSQETVSLTVKSMINGEESTLLLPLDKWQLDNKKPDPIKSLGIVPFIP
                     TIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKR
                     QGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQ
                     LTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALN
                     LLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
                     "
     gene            18443..20755
                     /gene="bamA"
                     /locus_tag="ACF2SP_001090"
     CDS             18443..20755
                     /gene="bamA"
                     /locus_tag="ACF2SP_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFD2358884.1"
                     /translation="MKKVSNKLILGVCCSTLLAWSSQTFSSDTFIVKGIRVNGLQRVS
                     TGTVLNYMPVQVGEEISSSSTAQIIRALYETGFFQSVSLERQGNVLVVNVVERATIGS
                     ITVVGNKEIPSDKMKAFLKEMGLVKGRVFQRSSLERLEKELKQAYTARGKYNSRIETK
                     VTPLTENRVAISITVSEGRVSRIKEIKIIGNHDFKANELLPELTLTTSNLFTYFTKKD
                     QYSKPGMDASLEALRSFYLDRGYLKFNVVSSQVLLSPDKKDVYINIHIEEGPQYHFSG
                     YDVVGKTILPKEKIDSLIQVKKGDVFSRKKVTESISAIGLALGDVGYGFPAINAEPRI
                     DENNKTVFITFVVQPGRHVYVRRINFHGNTKTGDYVLRNVIRQDEGGLLSLHNIKESE
                     RQLRMLGYLKNIDVKTTPVPGTNNQVDLDVNVEEAPSAEASASMGYGTNGYQFNASVN
                     QRNFMGTGRSMGAAFNASQWGQDYSFNYYNPFYTDTGVGRGGSLYYSRIDPKNLNVST
                     YSSNRYGGDISYNFPLGEKSSFQLGYGYQDINIKSVGYVLPIINFVALNGNHFQEIRL
                     TSGWSRNSYDQMPYPNQGFNQQAIAMVALPATSQSLSYYKSSYQAHLYYPLTRGWIFS
                     VLGNVGYGNTFDNFGLPFFENYYAGGPVQPGQVRGYDSYSLGPQDNFGNAMGANFLVN
                     GSVGLILPYPLSRDNVRTTIFADAGNVFASGTPPALRGTPAGPMRYSAGVSLEWRSPF
                     GPLSFSLAKALNPQPLDQTQLFQFALSSGF"
     gene            20869..21369
                     /locus_tag="ACF2SP_001091"
     CDS             20869..21369
                     /locus_tag="ACF2SP_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFD2358885.1"
                     /translation="MKRLGAVLVALFLGLFGTTVFADWAKIGVVDLQKIMQTSNQMKE
                     IQQKLEKEFKPRRDKLVAMEASLKSDMEKFKRDSAIMSASQKKELEKKIVASQQQFER
                     DGQQYQQELSTAHNEAMEGLYNKVRTAITKIAKDEKYDIIVQKDAAPFSSESLDVTDK
                     VIKAIN"
     gene            21383..22393
                     /gene="lpxD"
                     /locus_tag="ACF2SP_001092"
     CDS             21383..22393
                     /gene="lpxD"
                     /locus_tag="ACF2SP_001092"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFD2358886.1"
                     /translation="MLTLEELANYLNGVWHGNANHAIFSLSSLARASSKDLAYFDNPL
                     LQSVLNSTRAGAVLLKPEHKDWCPVNCIVVPNPSEAMFRAAKSLPHLATNVCGIHPTA
                     QIHKSAQIGQYVSVGANSVIGENVQLDDYVTIGSNTTIESSVLIGRGSQLGSGSIIHS
                     GTVLGQSVIIDSGCIVGAAPFNCYKEHGVWQQAPNFGGVVIGQRTQIGANTVIHRGSI
                     GDTYLGDGVCIDSLVLIAHDVYIGNNTAIAGCAAIGALVQIGSDCIIGGASCLAANIR
                     LTNDVVITGMSTVTKSIARSGVYSSGTTVHDHRKWRRNAARFRHLDDYITKLITIEKK
                     IE"
     gene            22521..22973
                     /gene="fabZ"
                     /locus_tag="ACF2SP_001093"
     CDS             22521..22973
                     /gene="fabZ"
                     /locus_tag="ACF2SP_001093"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFD2358887.1"
                     /translation="MNESIDINQIFTLLPHRYPFILVDRVIDYKVMEYLIAIKNVTIN
                     ENFFTGHFPGNPIMPGVLMLEALAQACGILANLSRQPKEGYEFLHYFAGIDNARFKQV
                     VIPGDQLRLEVRLAGQKRDFWRMHGEAYIGDKLACSADLLSAAKEIKK"
     gene            22970..23740
                     /gene="lpxA"
                     /locus_tag="ACF2SP_001094"
     CDS             22970..23740
                     /gene="lpxA"
                     /locus_tag="ACF2SP_001094"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFD2358888.1"
                     /translation="MIDERAMIHPSAKLASGVSIGPGTVIGADVEIGENTWIGPHVVI
                     EGPTVIGKNNKIFQFASVGDEPQDITYKGEPTRLEIGDNNVIREYCMISRGTVKGGGV
                     TRIGNSNYLMAYSHIGHDCMVGNHIIMVNYAALSGHVTINDYAIIGPYAAVHQFCQVG
                     AYAFIARATYVTKDVLPYVMIAGHTTSACGINTVGLRRRGFSSAAIDCLRRAYKIIFR
                     KGLTVQQAVSELELIQNECPEIIPMIDALNQSTRGIVR"
     gene            23744..24148
                     /gene="crcB"
                     /locus_tag="ACF2SP_001095"
     CDS             23744..24148
                     /gene="crcB"
                     /locus_tag="ACF2SP_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFD2358889.1"
                     /translation="MVVAPYLAVAIGGSLGAMSRYLVTIMAQNAWGIKFPYGTLLVNT
                     LGSFLAGFFLIVLVGRFSAEESFRLFLFTGFLGAFTTFSSFAAESLFMFEQGYWFKLI
                     TNILVNNVGSLSMVFIGTLVAKYVLLGHQGSN"
     gene            24170..25450
                     /gene="serS"
                     /locus_tag="ACF2SP_001096"
     CDS             24170..25450
                     /gene="serS"
                     /locus_tag="ACF2SP_001096"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFD2358890.1"
                     /translation="MLDNQLLRENPQYVATQLLKRGFQFDAVTFSQLEEKRKALQVST
                     QSLQNERNLRSKAIGEAKSRGENIEPMREEVNKLGAKLEQQKTELDEILKQIEVISLS
                     LPNIPHESVPVGKDELDNQEIRKWGDVPAFSFPVKSHDELGEALGQMDFALAAKITGS
                     RFVVMKGHLARLHRALIQFMLDIHIQQHGYQEIYVPYIVNADSLLGTGQLPKFEADLF
                     KLTGDNGYYLTSTSEIPVTNTVREMILSAEQLPIRYVCHSPCFRSEAGSYGKDTKGMI
                     RQHQFEKVELVWITKPEDSYNALEQLTQHAEVILQRLNLPYRVVALCTGDIGAGSAKT
                     YDLEVWLPSQNTYREISSCSNMEAFQARRMKARFRNPDTNEIQLVHTLNGSGLAVGRT
                     LVAIMENYQDEHGNIHIPDALKPYLGGIDIISVK"
BASE COUNT         7562 a         4505 c         5374 g         8205 t
ORIGIN      
        1 ccctcattaa aaacatgttt tacttcaagc ttgggttttt cataagccct gtaaaattaa
       61 aattgtagct acgaagcaaa taaatatgga aattccttca ttaaaatatc aagaactttt
      121 tgttgatcag atttactttt aataatgagc aagaccgtgt cgtctccggc aatagttcct
      181 aaaattcctg aattcgaaga gtcttttatg ctgaaggaaa cgtatttacg gtctatataa
      241 taagcaagac tgtttgcatt accaggatga gtttgcagaa tgattagccc aaaatcagaa
      301 atctggacat ttagaaccag aggtaagcca ggctgattgt aatcaatgat tttgtaataa
      361 ccggatactt ttgcaatttt catttttttt aagcgacgag aaagagttgc ttgaggaatc
      421 tcataccctc tttttcttaa gctgttctgc aaatcacttt gttccgcaat actttctgtt
      481 tgcactatcg ataaaatcaa atcatccaaa aaggcatcat gggtcaattg ctttctccct
      541 gttatcaatt caattgaata taaattacta aaattgaaat tatatccatt ttttcttgac
      601 agtacaaatc tcgatcatgt atattcaaaa aatgcataaa tatgggattc atattcaatg
      661 agaaaagttt ttagtttatt gttaattagt tcaattatag ttatatccag ttgtagtgag
      721 caaaaaaatc aaaatacact gcgtttttcc acttcagcag aatatccacc ttttgaatat
      781 attgaaaacg gagaaataaa aggatttgac attgatttgg caaaattgat cgccaaagag
      841 ctaggtaaag aagcagtttt tgacaacatg cagtttagtg cggttttgcc tgctgtgaat
      901 tcaggccagg atgacgttgc gattgccaca attactatta ccgaggaacg taagaaaaat
      961 tttgattttt ccaaacctta ttattttgaa ggaatggctg cagtttttga tacttcacag
     1021 ccagtgaaag agatctcgca attaacagga aaaaaaatag cagtccagct aggtagtgtt
     1081 atggaaattt ggcttcatca aaactttcct aaagctgaaa ttacggcttt ggataataac
     1141 aaccaagcga tagaagcttt aatcgcaggc catgttgatg tggtccttat ggatggggca
     1201 caagggggtg tttatagcaa gaaatatcca ggactgtctt attctatcct ggcgaaagct
     1261 aatgaaggtt atggattggt cttaaaaaaa ggttctcctc taagggatga aatcaattcc
     1321 gcattggaaa atcttaaagc gaagggtgaa attcaaaaat tggaatctat ttggataaag
     1381 gggtcaatat gacggagata gtgcaaaatc tgtttttcat tggcaaagga atagttctaa
     1441 ctatgcaatt attaacagga ggtatgctga ttggagtatt acttggaact ctcttgtcaa
     1501 tttgcagaca tcaaggagta gctggctttg ttataaaccg attcatatcc atattgagag
     1561 gcacaccact cattttacaa ttaagtttta tttatttcgc agtgcctgtt ttattaggta
     1621 tgcgaccaag tattttagtg gctggaattg ttgcttttgg tttgaatagc tcagcttatt
     1681 tcgccgaaat tttaagatcc ggaattgaga gtttacccaa agggcaattt gaagcagccc
     1741 ttactctgca aattcctacc ttttacttat ggaaagatat tattttaccc caagtgataa
     1801 aaaatatttt tcccgcaatg atcaatgaaa tgatcgcctt gttaaaagaa acggccttga
     1861 tttccactat tggaggcatg gatttaatgc gtcaggctca aactttggca gcacaacaat
     1921 ttacttattt tatgccttta tgtatcacgg gtttctatta ttacagtttg gtcttactcc
     1981 ttgaagcact aggtaaaaaa atcgaaaaaa gggggcatta tgctgcaagt cattaacgca
     2041 tcgaaaaaaa tcggcgatgt ccaagtactt aatgagatta atttaagtat tcaagcaaac
     2101 agtattgttg gtttagtagg gccttctggt agtggaaaaa caactttact gagatgcata
     2161 caacaattgg agtcaatcga ttcaggctct atccagtgtg aaggaaaaag cggatttatg
     2221 tttcaggatt ttcaattatt tccgcacatg acagtactca acaacctaat ttatgcacca
     2281 agtcttcacg ataaaactgc tgatcatata gcaaacgcat gggctttact tgctatgtta
     2341 ggtttgtcag acaaagctca ttcttaccca caaggattgt ctggtggaca aaaacaacgc
     2401 gtggccttgg ctagaagttt aatgatgaaa ccggatttat tgctttgtga tgaaccgact
     2461 tctggtctag atatggcgac cactaatgat gtcattgctc tattaaatac agtgaagact
     2521 ctaaaaataa ctatgcttat cgcttctcat gatttgtttt ttttatccaa tattgcggag
     2581 cgattgttgg ttgtaaggca agggcaacta attgctgact ttaaaaccca agaggtgtct
     2641 gatcctattt tatatctaca gcagttttac cagggggaaa tattatgaaa aaggttatta
     2701 aaaaaatcgc attagcttat tcaggtggct tggatacctc catcatgatc ccttggctta
     2761 aagaacatta tgagcatgct gaagtaatcg ctgtgatttg tgatttaggg caacaggaag
     2821 atctcgatgc cataaaaaat aaagcgctaa aaagcggcgc ttccaaagcg tatgtagtcg
     2881 atgtgaagaa tgagtttgcc attcaatatc tttggccttt ggtaaaatca ggtgctttat
     2941 atgaagatca atacattttg ggcactattt caaggccact aattgctcaa aaacttgttg
     3001 aaattgcttt gacggaggaa gtaaatgctg ttgcgcatgg agccaccggc aaagggaatg
     3061 atcaagtgcg ttttgaatat tcgattaagg cacttgcacc tcagcttgaa attattgctc
     3121 cctggagaac ttgggatatt aaatcaagac aggaagcaat tgtgtatgct aaagcgcatg
     3181 gcattgaggt accagtgact cccaaagcgc cttattctag agatcataac atttggtata
     3241 tttctcatga aggtggtgtg ctcgaagatc cttctcagga aatgccggat gacgtgttat
     3301 taatgactgc tcctgtatca cagactcctg atgaagagga agtggttgtt cttgatttca
     3361 aaaagggggt tccagtcgcc ttaaatggac aagaactttc tccagtggat ctcttaaatt
     3421 cattaaatca aaaagcaggg cagcatggta ttggagtggc ggacattgtt gaaaatcgat
     3481 tagttggaat gaaaattcga gggatttatg aagcacctgc tgctgcagtg ctttataagg
     3541 cacataaact tttggagagt ttatgtctca ctcgttctac gttacatctg aagcaatctt
     3601 tgcagcaaac ttatgccaat ttggtttatg aaggaagatg gttttctcaa accaaacagg
     3661 ctttggatgc attcattgat gtgacgcagc agcatgtcac aggctgtgtt aagttaaagt
     3721 tattcaaagg aaacattatc cctgccggta tgcattctcc ctatagcttg catcatcccg
     3781 aattggctac ttttgaagag gataatgtat acaaccaaaa ggatgcggaa ggattcatta
     3841 acctcttttc tttatccgct aaaatctata gccaggtaca tcagggggga aattatgact
     3901 aataaaacat ggggagggcg atttaagaaa tctttggata gtagtgtcaa ccagtttaat
     3961 gcgtcattgt cttttgatca tgtgcttttt gaccaggaca ttaatggcag tcaggttcat
     4021 gtgaagcaat tagctaaaca gaagatttta acagaagctg aatgccaggg gatttattct
     4081 gctcttgaag aaatcagaac agagataaaa caaggccaat attcatttaa tgaacgtgat
     4141 gaagaagaca tacatatgtt tattgagcaa ttgctgattc aaaaaatagg tgatttgggt
     4201 aagaaattac acaccggccg tagtcgtaat gatcaagtgg ctttggatct tcgtttatat
     4261 accagagata aaggctgtct gattaatgaa cttttgacaa ggcttatcga ttgtctagat
     4321 gatctaacca gtaaacatca acaggattta atgccaggct atactcattt acagcaagcc
     4381 caacctgttg cgttaggggc ttattttaac gcgtatcaat gcatgtttgg ccgagataaa
     4441 agcaggttgg atgattggtt taaacgcatg aattattctc ctttgggcgc cggggcactg
     4501 gctggaagta ccttgcctct tgatagagaa tgggtagccg aatccttggg atttgcaggg
     4561 atcattccta atactcttga tgcagtcagt gacagagatt ttgtcatcga gttatgcagt
     4621 gttgctgcaa tgattatgat gcatctttcc agattatgtg aagatttgat tctatggtct
     4681 acccaggaat ttaattttgt tatattggat gatgcgtttg ctacaggctc ttcactgatg
     4741 cctaacaaga aaaatcccga tgttcctgaa ttgattcgtg gcaaatcagg acgagtttat
     4801 ggtcatctaa tggccatttt aacggtaatg aaagggttgc ccttggctta taacaaagac
     4861 atgcaggagg ataaagaagg attatttgat acgattaata cgataatcgc ttgcctgcaa
     4921 atgataactc ctttcttgca aagtctgact tttaataccc tgttaatgaa aaccaaagct
     4981 caaagtggtt accttgatgc gactgccatt ctggagtcat tagtgatgaa gggtatgcct
     5041 tttcgtaatg cacaccatca agttggggca tggattgcag aagcgataga aaaacaatgt
     5101 tcactgaatg aattattaaa aggaggctaa gaatgtcttt ttccacctca accaatttga
     5161 ctccaattga aatgcaagac gatgaccttg aaaaaattca atctcagaat aacccatctg
     5221 caattaatga agtgaagacc aatacttttg tcattaattt acaacaagct atttttggct
     5281 tggagacagg tagtgaggcc gttgattttg attctcataa gaatacaggc tttccatttc
     5341 ctgctggtga atacaaacca agacattttc ttactggcac agagttagca agggaggaaa
     5401 ttcagtttgt cttaagccag gctcgctctc taaaacaaaa cccgggatta tataatcagg
     5461 cacttgccaa taaaagtttg gtaatgattt ttgaaaaacc ttcctttaga acccgtttga
     5521 gttttgcttt agcgattcag agtatggggg ggatcgttgt tgaaagcatc gctaactcaa
     5581 gaaagcagga gactccagct gatatggcta gagttttaaa tggttatgct gattttgtaa
     5641 tggtcagaac acatgaggac agttttttgc aagaaatggc cgaatattcc aaaatcccta
     5701 ttattaatgg cctctccgca ttgcatcacc cttgtcagac tcttgctgac ttgttaagtc
     5761 ttgaagaaca atttggtgct ttggaaggat taaccctcac ttacataggg gatggcaata
     5821 atattttgca tagtttgatg cttttggcac cgcagctagg tgtcactgtg cattattgtt
     5881 gcccaaaatc aaatcagcct aatgaggcca ttattaagga agcccaaagc aagacaggga
     5941 aacaaatgat ttattgtttt gataaaccgg aggaagctgt gaagcaagcg gatgccgttt
     6001 ataccgatgt gtggacaagt atgggatttg ataatcctga agcggtcaag atttttaaag
     6061 ggtttcaggt taatgagaca ttaatgtctc atgcaaaacc cgattcggtg tttatgcatt
     6121 gtatgcctat ggaaagggga aaagaggttt ctgctacttt acccgacagc gcgtcatcta
     6181 tcatttttag gcagagtgag aatagaatgc atgtacaaaa ggctttgatg ttatttttgg
     6241 atcaatagaa ccttggatta tgaggcccat atttgtgcat aataaacgct attgcgcatt
     6301 cctcctgata tgggctcatt atttttgcaa attaatttag taatttttct caaaggcgat
     6361 taattgttgt tccagactcc attgcaattt tgctttttcg ttgtgctcga taaatttttg
     6421 acagcttttg cttccaaggt catggcaatc aggaatcaat tcagctttat ccgcattagc
     6481 ccatatactt tttccaggta aaaaggagta agttaatgta tttctattaa tttgttttaa
     6541 ttgctgataa ttaaggccat gctctttggc tgcctctaca tattgttggg ttaagtccgt
     6601 tcttagcact ccttcgtcat cagtggataa aacgactggt acattatgag taagataata
     6661 attcagagga tgattgcggc cagatatatt cagaattttt aaattactaa tcaaattgat
     6721 ttcaacgggt atgtgatggt tagccatgta ttttaaggta tcttctgcat tgttttcata
     6781 accgatatcc acaccgtggc caattctttg ggcgtgccca atcagcagtg catcgcgaat
     6841 atgattactt agattttctg gtgtcactgc ctgaggtgat aattctccgg catgcaaggt
     6901 aataggaaca ttcgggtata ttcgatgcag atattcaaag atggacatgt gcttatggta
     6961 atcacgcaaa gaaataattc catcttccgg ttgcacgagg tttacgccga ttaagttccc
     7021 cttagacttt gagacggctt caaaggcatt caaggcttgg gcaaagacat tatcaacggg
     7081 ctgttctctg agtacataat aaataaattt aatttttaca aggcaggaat cggaagaagg
     7141 gttatctttg cagcctaatt cttgagatgc tttagcaaga attcggtcac tttccaaaac
     7201 agtatttttt atgttgcttt gaaaatcttt attcgctaat agcaggtgcc ttttctggtc
     7261 atatgatgga atgtccttga ttaaatcccc ataagttaat gagtgggcat tgtccgggat
     7321 tatcataatt tccatgtatt gctctttttg ctgtgctgct cgctgaacaa tatcggcaag
     7381 caattgaggc ctataatcaa aaactatggg catatatttg acaaatccat tgaaaaaatg
     7441 atcgtggcca gattctttac caggtataaa gtctttcata gaccagtctt taattatgtt
     7501 ggaatagagt tcaggattgt taaaaacctc tttaacatta actccctcac aatgagaaga
     7561 atctttactt acggcaaatg agtgtttatc gaggcaatag ttgccatttg cggcaagtgc
     7621 cagcatggtc tcaggatagg ctcctccagc caaatgataa tgtaactcgc cacccttggg
     7681 cattgtttta aaaaaggcat aaagggcact ggaatccttt tttatatgac taaaatagga
     7741 atcaacactg gcataaattg ggcacaaaag gaagaaactg gtaaaaaatg tgagtaatct
     7801 ggtaatgttc ataatttcaa tgtgatcatt aattgggcag atattatctc ataatcttgc
     7861 acgattccca acagaatatc tgttttatca tgcaatctct tatgattttt tacgaaatga
     7921 taatgattgt atttttttat tattcattgc agaatgtttt taaggtttaa cacatggaag
     7981 aaaaaaatgc cctatttcta tacaagatca gcgcctttgt tttttgtatt tgttatcgca
     8041 cttattttta taaatttttc ctatgcccat gaatttcaaa ctgattcaaa aaataaacat
     8101 aaagtggatg ttcacatagg tatttatgct ccgttttcca gtcattcagc ttttattgga
     8161 agaaatattc tggctgcaat ggaaatgggc agtgaacggt tacagtcctc tgatattcat
     8221 tattcatttt atactctgga tcaacttcct aaaaataaga atgctggtct tgtgctgcaa
     8281 aaatttattg atacccatca tatcaatgtg ttggtgactg gaggtaaaga aacggggtta
     8341 atagcagctc ccatagcaaa gaaaaacaat ttgattcatt ttagtctgac taacaatact
     8401 aaagttgcag acggcagaaa taattttatt gtctggagtc cagtttatga ccaagccaag
     8461 gttttggtta acgagctaaa acgtcagcaa gtccatcact taggaattat tacggcaaat
     8521 caaccttcct caaccgcttt gacgcatagt gttataaaac aattacaggc tgattctcat
     8581 attgatgtgg ctgcctccga gttttttgaa cctggaaata aggatttttc aagattaact
     8641 agtagaatta aaagaaaaaa tgcagatatg tatttggtaa tggcatcacc ggaggatatt
     8701 aaattaatac aagaacaaat ggaggcagat aaacagcatg tacctattac cagtatagtt
     8761 gagcgtgcta ctccagaagt ggtaaaggta tttgaaggtc aatggtatgt tgattctcat
     8821 gagatgcagc ctgattttat taatgaattt aaacaaaatt atttaaatta tcctgtaact
     8881 gaggcaggtt acgcgtttga tttatttcag cttttgaaca aaagcatagt aatggcgata
     8941 agaacacaag gttctcctac aagacaggaa gtggttcatc aactccatga cattgccaat
     9001 agcacaggtg taatgggaac tatggtattg gacactaagg gttctttatt taccaaatct
     9061 gaagtgaaaa ctgtgaaaaa tggcgagatt catactgtct aggtagaaaa taaaacctga
     9121 aatcatcagc tcattgtgaa tcgatgattt caggcgggag tgactaattg gtcattatct
     9181 gttagctaac gccattcctt ccaatacggt gagtgccgca tataactgat aatcctcgtg
     9241 tattaaatct ccggtttgaa tttggttgtt tttggattcg gtgttttctg aattgtcttt
     9301 gttaatcaag tgcccattaa ggtccgcttc agtaaaacca gccagagtgt cttttttgac
     9361 tgcattttta ggaacgtcta cttcttctac aatgatgtca ggaattattc ctttggcttg
     9421 aatggagact cctgatggag tgtaataaag tgctgtggtt agttttattc cggttttgcc
     9481 atcaagaggt aatacggttt gaacggatcc ttttccaaaa ctctttgtac caaggattat
     9541 agctctttta ttatctttta atgctccagc gacaatttct gaagcagagg cagaaccatt
     9601 attaattaag acaacgatag gcgcattgtc taacacatcc cctggattgg ccagggcggt
     9661 gaatttagag ccaggtagtc tgccttccgt atagactatc atttcttgct tacctgcctt
     9721 gtcattgcca agaaaagcat cggatacctg gatagctgaa tcaagtaagc ccccggggtt
     9781 atttcttaaa tccaatacta atccttttaa tttcccgcct gcttgttgtt ttaattgctc
     9841 gattgcttta atcatgtctt ttccagttaa agcctgaaat tgagtcaatc gaatgtaacc
     9901 ataaccttcg cttagcattt tgctttttac actttttatt tgaataactt cgcgaattaa
     9961 gtcaaaagtt aaaggtttgt ttacaccttt tctcaagata gttaattcta tggtagtgcc
    10021 tggtttaccg cgcattaaat tgacagcgtc tttaagagaa agaccttgaa ctgactcttt
    10081 acccagttta ataatataat cgccagattt gattcccgct ttaaatgctg gagtgtctac
    10141 aagaggagtg attactttaa caacaccttc ttccatggtc acttcgatac ctaaaccacc
    10201 aaattcaccg ctggttgatg tttgcaactc cttgaattcc tcttcattga gataactgga
    10261 atgcggatca aggccggtta gcatcccgcg aattgcgtta tcaaataatt ctttatcatc
    10321 aactggtttg acatagtatt tctttatttc accgatggca ttggaaaacc gttgcacatc
    10381 ttccaacgga attcgttttg tattagaata atttgaattg gtttcttcag ccgaaaacgc
    10441 ggtcaaaggc agcatcagag ttagggcata aaccaatgcc aaagtactgg aaaaaagttt
    10501 cttaataacc atagcgatct cctcagatgc aaggccatga aattagccac ctgcgtattt
    10561 ttttatgcaa tttaaatgcc agcggctaag ctttaagaca accagtctaa cggattgacc
    10621 gcttttcctc tttgtcttat ctcaaagtat agaccgtttt gcttaattcc accggtatgg
    10681 cctacacttg cgatttgctc gttttgttgg acgatttgtc ctttgcgttt gaagagagat
    10741 tgattgtgtg cgtataaagt cataaaccct tggccatggt caataatcag aagcaaacca
    10801 taacctttta gccaatcact aaatacaact ttccctgggt aaacggcagt aactatggag
    10861 ccttcatcgg caaaaaatgt cactccttga ttcatcctgc gtaaagatct atgggtggtt
    10921 tggacaggta acggcaattt tttgcgcatt cccgtgaacg gtttgccgct ttgaattaca
    10981 ctttgttgag ataatgactt catgagatga gataagttgt ctctatcttt ttgaaaatct
    11041 tttaatgatt gttgattatt ttgaatgtca ttatcaatgg attgaatcag tgtgctatgg
    11101 tatcttttat tttgctctag ttgtttttga ttttgtgaca acttaaattg tagctgcttg
    11161 ttttctgcaa gttcatttcc aagcttggtt ttactgtcgt taagattttg tcgggttcgg
    11221 gcaatttcat caatcaactg ctgacgtgat cttattatgt actgataata agttaaaatt
    11281 cggttgatct taaaaggctc atcttggtta atcaaccatt tcaaaggttg atactccccc
    11341 atctgataac gtgccctgac atgatttgcc agcatttcct gctgagcttg caatcgttta
    11401 tttaagtgat ttattttccc ttgcagttcg gcaatacttt tttcttttgc ggacatattt
    11461 gcttgtatga ttcttagctt gcgtattcct tctccaattt gtttttcagt gctgcccagc
    11521 tcttggttta gcacagtccg tttatcctgt gcattggcta aaacttgctt tagtttgtta
    11581 atcttggcat ctaactgttt tagtttggtt tgtgcttgag ttatagaatt tggagcagct
    11641 tttgcctcaa ggccattaca aaaaaataga atgaccataa gtgccaaacc aagagaagca
    11701 ccggataaag agttggatag gctaggcatg tgcatggttt ttttccagaa ggatacgacc
    11761 agtcatttcc ggaggcgggg ttatccctag taatgcaagc aaagaaggtg ctatatcgat
    11821 taaacttcct tcggaatgag tgaagtgcca ccctccacct acataaagaa aaggaaccgg
    11881 ttcactagta tgtgctgtat gagcttgatt tgtcgcttcg ctaaacattt cctcagcatt
    11941 tccatgatca gcagtgatca ataatttacc cccattgttt ttaagggctt gccaaacctg
    12001 ctgcatgcac tgatcaaggc attcaatagc ttgaacagta gcttcaaaat ttcccgtatg
    12061 tcccaccata tcagcattgg cataattaca aataatgaca tgataatctc ggctattgat
    12121 ggcggctatt agagttttgg ttaattcatg cgcactcatt tctggttgca gatcataagt
    12181 tgccacttgc ggagagggta ccataatgcg ttcttcattt gtaaaaacac tttcacatcc
    12241 gccattaaag aaaaaagtaa cgtgagcata tttttcagtt tcagctatgc gtaattgact
    12301 gagaccatga gaagacaaga cctcacccag ggtattattt aaaggaacag gtggaaatgc
    12361 tgtcgtggta aataaatttt tatcatattg ggtcatggaa acaaaacagg acagctttgg
    12421 cagtgttttt ctttcaaatc ctttaaacaa aggatcaagg aatgtagagg ttaattgcct
    12481 tgctctgtca gcacgaaaat tgaaaaacag gacagcatcg ccatcttgta tagaatgctc
    12541 tttccctata agagtgggtg gaacaaactc atcagaaatt ttatttttgt aataaaaatg
    12601 tattgcagtt tctgcatcag gaaattggcg ctcacttttg ccttgtgtta acaaattata
    12661 aacaggctct actctctccc aacgtttgtc tctatccatc gcataatatc ggccacaaat
    12721 tgaattaatg gttgcaacag ggtgtttagc cagttcttca ttcaggcatt tcaagctatc
    12781 aagagcactt tgtggtgggg tatctcggcc atcaagaaac aaatgcagat ggacggatct
    12841 gaattttttt tgattacaaa gggcaagtaa agcaaacaaa tgttgttcgt gactgtgcac
    12901 tccgccggga gagagtaacc ccatgatgtg aagagatttt tctgttttct ttaattggtc
    12961 aattacttca tggaaaacag catttttagc gaattttccg ttatttatcg cttcattaat
    13021 gcgagtaaag tcttgctgta tcactcggcc tgcgccaatg tgcatatgcc caacttctga
    13081 attacccatt tgctcatcag gtaaaccaac aggcaagccg gaggctttca aaagaatatg
    13141 agggcaggtt tgccaccatt catcccactg aggggtattg gcttttgcaa tagcattgta
    13201 tctatcattt tcgttatagc cccagccatc aagaatcatg agtaccaggg gagcattatg
    13261 cgacatgacc taaatcctaa attgtacaat gaaagggcta aaatgataat tataccttcg
    13321 cggcagtaag aggtatatag tttcagtaag agttcgctgt tattaagttt agtagcaatc
    13381 ttctttttgt tcgcagtgac cataagcatc gcatgactgg atcggtcgac acccagggta
    13441 ataaataatg ggtacaggag ttgtaataat taaaggagga gtagttgatt tatcacagga
    13501 tttacaatga ttaaaactgg ctcttacatc attctctttg ggtagatttt ttggatttgg
    13561 tcctatccct aatagattga acgccatggc atgattaaca agagcagtta aactgataaa
    13621 taaaataatt gctttgagaa tcaggtttct agtatcccta ctcatgatgt gaactccttt
    13681 ttaattttat gccccgtatt atgcgataat aagagtatag tttcttgtta tggttctgct
    13741 actataccaa aggattatct tgcaattctt ccggtacata ttgagacaaa tatttgctta
    13801 aactcaattg cacagtcgag gttatcccat attgcgtttt taacaataat aaagtactcc
    13861 agccccacca actgtttgtc caatgcctgt catatggatt tgtcaaattg gttcttctta
    13921 acaactgttt gttttcttta tcccaataga acataggagc aatatccatg ttattttcat
    13981 catttaattt tgtgatgtgt ggaatcatgt cctctatttg atttttttct ggtagaacgc
    14041 ctgagtaata agcttcaaat atttcatcag cggaagcttg caaggctttt aagagcatct
    14101 gctggttggt ttgattaaaa ggattaaagt aagaataatc accataaacc atccattgtt
    14161 ctcccagatt attcgtcaca tgaattccgt acttattttc ttcattatgc atataattgg
    14221 ataacaacgc accaaggact gttggtgtga taatggctga taattcttct ctaggagtgc
    14281 gcaaatggcc ggcagcaaag tgatctgata aatagtggca ggcaaaagca tccatcgcat
    14341 aggcgatttc caaatcagag cgttttcctg ttcgttttgc ttttaatgcc tgttgcagtg
    14401 cgacatggtg gccactttta taggctatca gattatttgg cgtaaagtga tcatagtttt
    14461 ctatggctag ttttagatat ctgccaggat ttaaccacca ggtcaggcta ttacaaccac
    14521 ctcccgtgat gcagttgatt tggcgaccta cttcattccc aattcgttta taaatggctt
    14581 cagcagtttc tccttttgca atccctgatt cgacttcgcg catttctgct ctgataacgg
    14641 aattaagatc ttctacctca ctaactgcaa taacattcct ggcaaaactg ttgaaaacct
    14701 ctttaaaacg agtttgtttt tccagttttg ttaagccatg actaattggt tttcctacaa
    14761 ttccatacag atcacccaga cttatgatat cgccggctgt tagtgccaat ccatttttta
    14821 aatgtaatac agcacccgat tggggaacct catttatgtc aaatcgtagt ttgatttgtt
    14881 gccccatgga ccaatgctcg gacaaagcaa aactgccgtt cgattcacca taaattatag
    14941 aggaaaaagc caacaagcta atgctcattg caaataaccg aggctttact aatcgatgga
    15001 tagatttttt cattatgtcc tcatcaacag ggattgatca ttgggtttag tgagaaatag
    15061 cgtattgtat aacatttgag atgaaatatt gatgcattgg ttcgtcccga gataatgcat
    15121 aggtatgaat ggagtctgta caaataaccg taaccaaaaa cgacttagag cgctataggt
    15181 catgggaatt atctcctttc tgggtattca tgatgaaaag tgacaactcg ttacgaagat
    15241 tttttcaggt ttaataatga ggtgtttcat aaacaaggtc aattgatttc ttaagtagta
    15301 cttaaatttt tatctcaaag aggttagact atgcatcatt tttattagtc ggataagttg
    15361 ttttgaatca taaattacct caacacattg ccgttgtcat ggatggaaac gggcgttggg
    15421 ctgaaagccg cggtttgccc cgtgtagagg gacacaaagc gggccttgac tcagttaaaa
    15481 agattatcaa ttgttgcctg gaaaaaaaaa tttcctgctt aagcctgttt gcatttagct
    15541 ctgaaaattg gtcaagacca gtgacagaag tcaattttct tatggaattg tttttggagg
    15601 ctttgcgaaa agagatagac gatttaaacc agcatggaat acggttaaag tttactggcg
    15661 atagagaacc tttgtctcaa gtattacaaa agcaaatgta tgatgcggaa gctcttacca
    15721 ggaataatca acaattaatt ctcaatgttg ttgtgaatta tggtgggaaa tgggatatag
    15781 tgacggcggc taaaaaatta atacactctg ttttagatgg gaagttggca tgtgatgaaa
    15841 tcaatgaggc agtattcgct caatttcttg atacgagtgg tatgccggaa cccgatctgt
    15901 ttattcgaac tagtggtgaa ttaaggataa gtaatttttt tctttggcag ttagcttata
    15961 ctgagctgta ttttactgat gttcattggc ctgattttaa tgagcatgag tttgaattgg
    16021 cattgacttc ttttgccagg cgggcaagaa ggtttgggca aatttcacaa tctgaataaa
    16081 ggtaaaacca tgtttatgca acgattgatc acgacattaa tattagcgcc cctggttttg
    16141 ctagctattt tttatgctcc ttcctggatt ttatggggta ttgttctagt tatactcctg
    16201 cttgcaggac tggaatgttt caaattgatt cctgttgaca atttaactgt acaggcttgt
    16261 tttttgatat ttttattgat gtgcttttgg gccagtggct atttctatcc ctattggttg
    16321 agtgtgggct tattggtttg gttattaaat gggttagcaa ttcttactta tccgcgttca
    16381 caaaattatt ggggttatcc aatcgttgtt gccatagctt ctttgatatt actaccctta
    16441 tttactcaaa gtcttattca aatttctttt ttacagcaag gaaaggaatt acttgtctat
    16501 ttgctttttt taatttgggc ttctgatatt ggtgcttatc tcgctggaaa acaatgggga
    16561 aaacataaat taatccctaa agttagtccg ggtaaatctt gggaaggtgt tacaggcgga
    16621 gttattttag ccatgatagt cgcgactttg ggctattatt attttatacc ttactcaatt
    16681 tatatatggt ttggtttagc attgtctact gtagttattt caatttttgg tgatttgttc
    16741 atcagtatat taaaacggcg ttgtcaatta aaagatactg gaaatattat tccaggtcat
    16801 ggcggtatat tagatcgact ggatagtcta attgccgcgt taccatggtt ttactttgga
    16861 ttaacctaca taccactggg aatttaattt tccggtcgta ttaatctttg gataacgata
    16921 tgctttcaac attgctgtac tttttattag cattggtatt gttagttacc atacatgaat
    16981 acggccactt tcaagtagcg cgatggtgtg gtgtgaaagt gttaaggttt tcatttggct
    17041 ttggaaagat tcttgcgcgt ttttatgata agaaaggaac agagtatgcc tggtctttgt
    17101 ttcctttggg tggctatgtc aaaatgcttg atgaaacgga aggagaggtt tctgaaaaag
    17161 aaaagccttt cgcattcaat aatcaatctg ttcttgtcag gatagctatt gtagtggcag
    17221 gacctttatt caattttatt tttgcgtttg tagcattgtg gttggttttg gttatcggca
    17281 tgcactcttt ggcaccgatg atagagtcag taagaccaaa tagtattgct gcacgagctg
    17341 gcctagcccc caagcaggaa atccttgcac ttaatggagt taaaattaat agttggcgag
    17401 attttcaata tgaaataatg cctcttatcg gttcccagga aacagtgagc ttgactgtaa
    17461 agtcaatgat aaatggtgaa gaaagcacgt tgcttttacc tttggataag tggcagttgg
    17521 ataataaaaa gcctgatcct attaaaagcc tgggcatcgt tccttttata cccactattc
    17581 cacccatagt cggtgaagtg gttcccgatt ctcccgctga aaaagcaggc ttaaaaatag
    17641 gggatgaaat tattagtgtt aatggtcagc atttcaatga ttggttgtat ttagtcagct
    17701 acgtaaggga aaggcccaat tctcaaatca atttggatat taaacgccaa ggtaaattgc
    17761 tgaagataac ggtacacact ggaagtcagg ataataatgg aaaattagag gggttaatag
    17821 gagttagatc tcagaaagta gattggcctg ctcattggct acggctggag caacagccgc
    17881 caatttcagc acttggtacg gcttttaagc aaacagtaca actcacaggc acaactttta
    17941 tcttaatggg aagattagtg actggaaaat tgggattaaa cagtattagc ggtcccgtcg
    18001 gtatagccca gggggccggc gactccggac gcggaggatt ggtatcctat ttattttttc
    18061 tggctttagt aagcattagc ctgggagcgc ttaatttatt accaattcct atgctggatg
    18121 gcggtcatct tttgtattat gtcttggaaa tcataagaag aaagccatta tcggacggag
    18181 ttaaatctgt tgggatctat tttggattat tactgttggt tgccttaatg tttgttgctc
    18241 ttagtaatga tatatcaaga ttaaccagtt aggatttagt gagagtaatt ctaatttctg
    18301 tatgattaat gaatgaaatg atgttttagc gacaaagaaa cttgacagta gtttctagtt
    18361 cctataaaag ggtttcaatt ttttgtacaa ttgaatggtt tttaatttca taagtgcgcg
    18421 tagtgctgga cgtaaaataa taatgaaaaa agtcagtaat aaattaatat taggtgtttg
    18481 ttgttctact cttttagctt ggtcatccca aaccttttct tctgatacct ttattgtcaa
    18541 agggattaga gttaacggat tacaaagggt ttcgacaggt acggtattaa actatatgcc
    18601 tgtgcaagtg ggtgaggaaa tcagttccag ctcaacagct caaattatcc gcgctctcta
    18661 tgagacagga tttttccagt ccgtttcgct tgaacgccaa gggaatgtgt tagtggtcaa
    18721 tgtggtagag cgagcaacta ttggctctat taccgttgta ggaaataagg aaataccctc
    18781 tgataaaatg aaggcttttc ttaaagaaat ggggctggtc aaaggcagag tatttcaaag
    18841 atcttccttg gagcgtttgg agaaggagct gaaacaggcc tacacagcca gagggaagta
    18901 taattctcgt attgaaacta aagtaactcc tcttactgaa aatagagtag ccattagtat
    18961 tactgtatca gaaggtcgag tttcacggat taaagaaata aaaataattg gtaaccatga
    19021 ttttaaagca aatgagttat tgcctgaatt gacgttaact acaagcaatc tgtttactta
    19081 ttttactaaa aaagatcaat attccaaacc aggaatggat gcttctttag aagcattacg
    19141 ttcattttat ttagataggg gatatttgaa atttaatgtt gtctcttcgc aagttttgct
    19201 atcgcctgat aaaaaagacg tctacatcaa tattcatata gaagaaggac ctcaatatca
    19261 tttctcaggt tatgatgtgg ttggaaaaac gatattacct aaagaaaaaa ttgattcact
    19321 gatacaggtt aagaaaggcg atgttttttc tcgtaaaaag gttactgaat ccatctctgc
    19381 aatagggtta gctttagggg atgtaggata tggttttccc gcgattaatg ccgaacctag
    19441 aatagatgaa aataataaaa cagtatttat tacttttgta gtgcagccgg gccgtcatgt
    19501 ttatgtgaga cgtattaatt ttcatggtaa tacgaagaca ggagattatg ttctacgtaa
    19561 tgtgattcgt caggatgaag gtgggttact gtctttacat aacatcaagg aatctgagcg
    19621 ccagttacga atgctgggtt atctaaaaaa tattgatgtc aaaacgacgc ctgttccagg
    19681 aaccaacaat caggtcgatt tggatgttaa tgtggaagaa gctccttcag cagaagcaag
    19741 cgcatctatg ggatatggta caaacggtta tcaatttaat gcatccgtta accagcgcaa
    19801 ttttatggga actgggcgtt ccatgggggc tgctttcaat gcaagccaat gggggcaaga
    19861 ctactctttt aactattata atccgttcta taccgatact ggggtaggcc ggggaggaag
    19921 tttatattac tcaaggatcg atcctaaaaa tttaaatgtc agtacgtaca gttctaatcg
    19981 ctatggtggc gacatcagct ataatttccc gttaggtgag aaaagcagtt ttcaattggg
    20041 atatggttat caagatatca atatcaagtc agtgggttac gtactcccca taatcaattt
    20101 tgttgcttta aacggtaatc attttcagga aataaggttg acatccggtt ggagtagaaa
    20161 cagctatgat caaatgcctt atcctaacca aggatttaat caacaagcta ttgctatggt
    20221 cgcattgcca gcaacatcgc aatctctatc ttactataag agctcttatc aggcgcattt
    20281 atattatcct ctaacccgcg gctggatttt ttctgtcctg ggtaatgtgg ggtatggaaa
    20341 tacttttgat aatttcggat tacccttttt tgaaaactac tatgccggtg gtccagttca
    20401 accaggccag gttcgagggt acgatagtta ttctttaggc ccgcaagata attttggaaa
    20461 tgcaatgggt gcgaatttcc ttgtgaatgg aagtgtaggc ctgatactac catacccatt
    20521 gagtcgagat aatgtaagaa cgactatttt tgctgatgca ggtaatgtat ttgcttccgg
    20581 gacacctcct gctttacgtg gaaccccggc aggtccgatg cgttattcag ctggtgtgtc
    20641 attagaatgg cgctcacctt ttggtccatt gtcttttagc ttggctaagg cattaaatcc
    20701 acagccattg gatcagactc aactcttcca atttgctctt tcctcaggtt tttagaggta
    20761 ttatggatta gggtaagcag attatagtat tttaaaaact agtgctgtac atatcgcaac
    20821 ctttttcgaa ttgtgctagc atttcaaact ttaaattagg agatcattat gaaacgtctg
    20881 ggtgcggtgc tagtcgcatt atttttgggt ttgttcggca cgactgtgtt tgctgattgg
    20941 gcaaagatag gcgtagttga tttacaaaaa atcatgcaaa cctcaaatca aatgaaggaa
    21001 atccaacaga agttggaaaa agaatttaag cctcgtcgag acaagcttgt cgcaatggaa
    21061 gcaagtttga aaagcgacat ggaaaaattt aagcgtgata gtgctataat gagtgcgagc
    21121 caaaagaaag aattggaaaa gaaaattgtt gcgtcacaac aacagtttga acgtgatgga
    21181 caacaatatc agcaagaatt gagcacggct cacaatgaag ctatggaagg tttgtataat
    21241 aaggttcgta ctgccattac caaaattgcc aaggatgaga agtacgatat tattgttcag
    21301 aaagatgcag cgcccttcag cagcgaatct cttgatgtaa cagataaagt tatcaaagca
    21361 attaattaat ccggcggatg cgttgctaac cttagaggaa ctggcaaact atttaaatgg
    21421 cgtctggcat ggcaatgcca atcacgccat ttttagtcta tcctcgttgg ctcgagcttc
    21481 atctaaggat cttgcgtatt ttgataatcc cttattgcag tctgtcttaa actctaccag
    21541 agcaggtgct gttttattaa agcccgagca caaagattgg tgtcctgtga actgtattgt
    21601 agttcccaat ccgtcagaag caatgtttag agcagcaaag agcctgcctc accttgctac
    21661 caatgtttgc ggaattcatc ctacagctca aatccacaaa tctgcccaaa taggtcaata
    21721 cgtatcagtt ggcgctaaca gcgtgattgg tgaaaatgta caattggatg attacgtaac
    21781 tattggttct aatacgacca ttgaatcttc agtactgatt ggcagaggga gtcagctggg
    21841 ttctggatca ataattcatt ctggtacagt tttgggccaa tctgtaatta ttgattctgg
    21901 gtgtattgtt ggtgctgccc cgtttaattg ctacaaggaa catggcgtct ggcaacaagc
    21961 accaaatttt ggtggagttg taattggcca aagaactcaa atcggtgcaa atacagtaat
    22021 tcacagaggc tccattggcg atacttattt aggcgatgga gtttgtatag acagtttagt
    22081 cctcatcgcg catgatgttt atatagggaa taacactgct attgcaggtt gtgctgcaat
    22141 tggtgcttta gtgcaaatag gcagtgattg tatcataggt ggggcaagtt gccttgcagc
    22201 gaacattcgt ttgactaatg atgtagtaat aacagggatg tctacagtga caaaatccat
    22261 tgcccgctcc ggggtttatt cttcgggaac gacggtccac gatcatcgca agtggcgtcg
    22321 taacgcagcg cggttcaggc atttggatga ctacattaca aagctgataa caatagaaaa
    22381 aaaaattgaa taataacaac taattatgtt ttatcaattt gaagtaacac tatttttgaa
    22441 atttttaatt gattagaatg agctaaacca agataatgag ccattttgta atgcaatagg
    22501 cgattaatga gaactagggt atgaacgaat cgatagatat aaatcaaatt tttactttgt
    22561 taccacaccg ctacccattt attctggttg atagagtgat agattacaaa gtaatggaat
    22621 atttgattgc aatcaaaaat gtgactatta atgagaactt ctttaccggg cattttccag
    22681 gaaatcctat tatgcctggg gtactaatgc ttgaggcctt agcccaagct tgtggcattt
    22741 tagccaattt gtctcgccaa cccaaagaag ggtatgagtt tctgcattat tttgctggta
    22801 ttgacaatgc tcggttcaag caagtagtga ttcccgggga tcaattgcga ttagaggtga
    22861 gactggctgg acaaaaaaga gatttttggc gtatgcacgg agaagcttat attggagaca
    22921 aattggcttg ttccgcagac ttattaagcg cagcaaagga aattaaaaag tgatagatga
    22981 aagagcaatg attcacccat cggcgaagct ggcaagcgga gtgtcaatag gccctgggac
    23041 cgtgataggc gctgatgttg agattggtga aaatacctgg atagggccgc atgtggtaat
    23101 tgaaggacct actgtaattg gtaaaaacaa taagatcttt caatttgctt ccgtaggaga
    23161 tgagccacaa gatatcactt ataagggcga gccaactcgt cttgaaatcg gcgataataa
    23221 tgtaatcaga gaatattgca tgattagccg aggtactgtc aaaggtggag gtgtcactcg
    23281 cattggcaat agcaattatt taatggctta ttcacatatt ggccatgatt gtatggtagg
    23341 caatcatatt attatggtca attatgcagc cttatcaggt catgtcacaa ttaatgatta
    23401 cgcaattatc ggcccttatg ctgcagttca ccaattttgt caggtgggag cctacgcctt
    23461 catagcccga gctacctatg tcactaaaga cgtattgccc tatgttatga ttgcaggtca
    23521 taccacttct gcttgtggaa tcaacactgt tggtttaaga aggagaggtt tttcttctgc
    23581 tgcgattgat tgtttgcgac gcgcatataa aattatcttt cgcaaagggt taaccgttca
    23641 acaggcagtt tcagaattag agttgattca aaatgagtgt ccagaaatta ttcctatgat
    23701 tgatgctttg aatcaatcca caagaggcat tgtgaggtaa gcaatggttg ttgcacccta
    23761 tttggctgta gctatcggag gctctttagg cgcaatgtct cgttatttag ttacaataat
    23821 ggctcaaaat gcctggggga ttaaatttcc ttatggtact cttttagtga acacactagg
    23881 ttcttttcta gctggttttt ttcttattgt attagtaggt cgttttagcg ccgaggagtc
    23941 gtttcgttta ttccttttta cgggattttt aggagctttt actacctttt cgagtttcgc
    24001 tgcagaatct ttatttatgt ttgagcaagg atattggttt aaattaatta ccaatatact
    24061 ggtcaataat gttggctcat tgtccatggt cttcattgga acattagttg ctaaatatgt
    24121 attattagga catcagggct ccaattaata atttttagag tgtttgctta tgttagataa
    24181 tcaattactt cgagagaatc ctcaatatgt agcaacccaa ttgctgaagc gaggatttca
    24241 atttgatgcg gtcactttca gtcagttgga agaaaagcgt aaggctttgc aagtaagtac
    24301 gcaatcatta cagaacgaac gaaatttgcg ttcgaaagcc atcggtgaag caaaatctcg
    24361 aggtgaaaat atagaaccta tgcgcgaaga agtgaataaa cttggcgcta aactggagca
    24421 acaaaagact gaactggacg agattcttaa gcaaattgag gtaattagcc tatcattacc
    24481 taatatccct catgagtcag tacctgttgg taaagatgaa ttggataatc aggaaatcag
    24541 aaaatggggc gacgtacctg ctttttcttt ccctgtaaag tcacatgatg aattgggtga
    24601 agcactaggg caaatggatt ttgcgcttgc agctaaaatt acgggcagtc gttttgtcgt
    24661 catgaaaggg catctggccc gattacatcg ggctttaatt caattcatgc tggatataca
    24721 tattcagcaa catggttacc aggaaatata tgtaccttat attgttaatg cggatagctt
    24781 actagggact ggacagttac caaagtttga ggctgattta tttaaattaa caggtgataa
    24841 tgggtactac ttgacgtcta cttcagaaat tccagtgacc aatacagtaa gggaaatgat
    24901 attgtccgct gagcaattac ctattcgtta tgtttgccat tccccgtgtt ttcgtagtga
    24961 ggcgggttct tatggcaaag acacaaaggg aatgattaga cagcatcaat ttgagaaagt
    25021 tgaactggtc tggattacca aaccggaaga ttcttataat gctttagagc aacttactca
    25081 gcatgctgaa gttattttac agcgtttgaa cttgccttac cgagtggttg ctttgtgtac
    25141 cggggatata ggcgctggtt cagccaaaac ctatgatctt gaggtttggt tgcctagcca
    25201 gaatacttat cgagaaatat cctcttgttc caatatggag gcatttcagg cacgccgaat
    25261 gaaagcgcgt ttccgcaatc cggatactaa tgagattcaa ttagtccaca ctctaaatgg
    25321 atcaggttta gctgttggac gaactttagt tgccattatg gagaattatc aagatgagca
    25381 tggaaacatt catataccgg atgctttgaa gccttattta ggtggtattg atatcatctc
    25441 tgtgaaatag gtttgctcat aattatggat attttttctt aaaagttagc cttcaatagg
    25501 aaagagcgat agataggcta tattgagcca aatcaattgc tcttaagtat ttttaagaaa
    25561 aaaatccggt gactctaaac taatccaaat gaaacatttt gatttttcct taagaatctg
    25621 gccaaaaatc atagatatca atggaa
//
LOCUS       DAXLDT010000034        25287 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXLDT010000034 DAXLDT010000000
VERSION     DAXLDT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25287
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.034"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1381)
                     /gene="ankI"
                     /locus_tag="ACF2SP_001097"
     CDS             complement(<1..1381)
                     /gene="ankI"
                     /locus_tag="ACF2SP_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkI/LegAS4"
                     /protein_id="HFD2358891.1"
                     /translation="MIILYDFLQESSVMPRSKNDSNLKKKSALQSKFKEQQWNHGSKE
                     HKSKFKFTQRKAKKKGPGMTHLPGNIYTLFTPVNGLKNNAQLTDTSKIMVNLHIDNMS
                     SSDYIPSAIDRTDLVIVQPVHLLRKTGGRGLFAREDIPKGTCIGIYTGEVYSEQEFEQ
                     YLMEHVGSDKSYAMYVGGRVVDAARKGNLTRYINFSDSQDNAEFVETTLNRKKVVKVI
                     TTKNIKAGQQLLINYNTYEEQASRYYYFLNPGDGWLSAQEFYQTYQSQYRLEQMPYNL
                     EGFDLKAGDRILMTQIGRIIFANYSLAKEQELNASDIDLPFLKVGSDEKILDFDEADT
                     FTPLMAACYLGQVENVKWLIEHGANIDQQQSHSGHCPLSLTLKGYSLAKDTKKYIDII
                     QLLIKNQVNLLVHDRSDKTFLHNAALVLNNLDFQSVVKFLIGQNPIDINEYFTYIDEN
                     DFDIVMHCYN"
     gene            1525..2289
                     /gene="map"
                     /locus_tag="ACF2SP_001098"
     CDS             1525..2289
                     /gene="map"
                     /locus_tag="ACF2SP_001098"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFD2358892.1"
                     /translation="MGVTIKTPDEIEKMRVAGRLAAEVLEMIGPYVQVGITTDELNTI
                     CHDYIVNVQKAIPAPLNYNGFPKSICTSINHVVCHGIPGKKTLKDGDIINIDVTVIKN
                     EYHGDTSKMFIVGTPSVKAKHVVQIAHECLYIGIDMVKPGVQLGDIGHAIQQHAEKNR
                     CSVVRDYCGHGIGRVFHEDPQVLHYGVPGTGMKLEPGMTFTIEPMVNIGKHHTRLLPD
                     HWTVVTKDHSLSAQWEHTLLVTDTGVEILTLRNEER"
     gene            2276..4861
                     /gene="glnD"
                     /locus_tag="ACF2SP_001099"
     CDS             2276..4861
                     /gene="glnD"
                     /locus_tag="ACF2SP_001099"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFD2358893.1"
                     /translation="MKNDNRIIKNTIKQFKEKLCKDFSQKANITSITRKLAVFIDTIL
                     IQLFIKNKLHFGDNFCLLALGSYGRRELQLHSDIDLLILHTEKVSNIQLQRAQKFIQD
                     CWDVGLEVSHQITTVSSCANLASQDLSVISTIMDMFLLCGHGALMEELIYQTHTLHMW
                     PSHQYFFAKLQEQQNRYAKYGETAYNLEPNIKNGPGGLRDLQILLSISKRHFKIKKLA
                     EGIGYGFITDKEYEELKYCQNFLWRVRFALHMLAGKPEERLSFDYQVKLAQFFGYQDQ
                     SHILAIEQFMKDYFKVIKRNRELNEMLLQWFNETIVYHQKQKIIRLDDEFQLSNRFIE
                     VRNNRVFKQNPQSILKLFYWLVKRPDIEGVRASTIRLIRESLFLMGKRFRESKETANI
                     FINIFRTGNDPYDALQRMNRYGVLAHYLDCFATVTGQMQYDLFHAYTVDQHTLFVIRN
                     ISRFKKNEYAKQFPLCAKIISALEKPEILYLGALFHDIAKGRGGDHSELGAIEAQQFT
                     QRHYMEAEDSKLIVWLVRYHLLMSQTAQRKDIYDPKTIEQFCQLLPHARYLDYLYLLT
                     VADICGTNPTLWNAWKDSLLKELYHAAKTRLHKQQELLDEAALISIRKQYAMDILISD
                     GISSRVIQDLWSQFKGKYFLHESPEVIARHTKAILNSKQFPVVIIMPHHSQGGTEVFI
                     YMPHKDERFTITTSVLSNHHVTIQEAAIITCDNQFDLDTYIILDENNQAFLNEQRARD
                     IQKSLCDHLANTGRLPAVSRRRLSRALTHFNVKTQINFIDDNTNHQTQLFLVTNDRPG
                     LLATISRVFLTLNIHLHNAKIATAGERVEDMFYISNQTGYSLNHEEKTILKEKLILEI
                     SKSKY"
     gene            complement(4883..5326)
                     /gene="tadA"
                     /locus_tag="ACF2SP_001100"
     CDS             complement(4883..5326)
                     /gene="tadA"
                     /locus_tag="ACF2SP_001100"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFD2358894.1"
                     /translation="MIDKFWMQLAYEQAVIARNEGEVPVGAVLVSKDNQLLGVGRNVI
                     EKSHDPSDHAEIRAIRQASRKLNNHRLLDTTLYVTLEPCVMCAGLMVHARIKRLVFAT
                     RDFKAGAAGSMFNVLHALNHSVLIDEGIMQAECSQLLSDFFRNLR"
     gene            complement(5323..6900)
                     /gene="guaA"
                     /locus_tag="ACF2SP_001101"
     CDS             complement(5323..6900)
                     /gene="guaA"
                     /locus_tag="ACF2SP_001101"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFD2358895.1"
                     /translation="MNDLKKSPLLILDFGSQYTQLIARRVREMGVYCEIYPYNINHEQ
                     FKKLNPCGVILSGGPSTVTHDANPRAPQWLFESDLPLLGICYGMQTMAVQLGGQVHSS
                     TLREFGYAELRLHGHSQLLSNIEDRTAMDGSALLDVWMSHGDKVTELPPGFKVICETR
                     NAPIAGMADESRQMYGLQFHPEVTHTLQGLRILQRFVVDICKASTDWTPEHIIDEAIN
                     KIREQVGTEKVLLGLSGGVDSSVVAALLHRAIGEQLVCVFVDTGLLRLNEAEQVLSMF
                     GRHMGIRIIAVNAEDKFLTALKGVTCPEEKRKIIGRTFIEVFDEEAQKLTDIKWLAQG
                     TIYPDVIESAATSTNDAAVVIKSHHNVGGLPDTLNLKLLEPIRELFKDEVRHVGLELG
                     LPHDMVYRHPFPGPGLGVRILAEVKKEYADILRKADAIFIEELHNAQLYHKISQAFAV
                     FLPVKSVGVMGDGRRYDYVICLRAVETVDFMTAHWSQLPWDFLGKVSNRIINEVEGVS
                     RVTYDISGKPPATIEWE"
     gene            complement(6903..8375)
                     /gene="guaB"
                     /locus_tag="ACF2SP_001102"
     CDS             complement(6903..8375)
                     /gene="guaB"
                     /locus_tag="ACF2SP_001102"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFD2358896.1"
                     /translation="MPLSIVQQSLTFDDVLLVPAHSTILPKDVSLKTKLTRAIHLNMP
                     LISAAMDTVTEARLAIALAQEGGLGIIHKNMSIADQAEEVRRVKKFESGMVKDPISVT
                     PDLTVKELLAVMTKYNFSGVPVVDGKHLVGIVTSRDIRFETNMNLTVAQVMTPKGRLV
                     TVKEGASREEVRSLLHKHRIEKLLVVNENFELRGLITVKDIQKAKDNPYACKDSFEQL
                     RVGAAVGVGEGTDERVAALVESGVDVIVVDTAHGHSQGVLNRVKWIKKNYPDVQVIGG
                     NIATAAAARDLYEAGADAVKVGIGPGSICTTRIVTGVGIPQISAIANVAQELKGIIPV
                     IADGGIRFSGDVCKALAAGADTVMLGSMFAGTEESPGEIELYQGRTYKNYRGMGSIGA
                     MSLAQGSSDRYFQDASLGTEKLVPEGIEGRVPYKGPVQTIIHQLLGGLRSCMGYTGCA
                     TIEELHSKTEFVQVTNAGMRESHVHDVSITKQAPNYQVDN"
     gene            8584..9414
                     /gene="minD"
                     /locus_tag="ACF2SP_001103"
     CDS             8584..9414
                     /gene="minD"
                     /locus_tag="ACF2SP_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFD2358897.1"
                     /translation="MAKIIVITSGKGGVGKTTSSAAISSGLALLGHKTVVIDFDIGLR
                     NLDIIMGCERRVVYDFINVINGEANLNQALIKDKRIPNLCILPASQTRDKDALTLEGV
                     EKTLNELAKDFDFIICDSPAGIETGALMAMYFADHAIVVTNPEVSSVRDSDRILGILA
                     SKTKRAIENKAPVQEHLLLTRYDPERVERGEMLSVTDVKEILAIPLIGVIPESKSVLK
                     ASNTGTPVILDESSDAGIAYQDAIARFLGEERPMRFISIDKKGLFRRLFSKNKEEVTI
                     "
     gene            9411..9680
                     /gene="minE"
                     /locus_tag="ACF2SP_001104"
     CDS             9411..9680
                     /gene="minE"
                     /locus_tag="ACF2SP_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFD2358898.1"
                     /translation="MSIFNYLRRRASTASVAKERLQIIISHERSQRNTPDYLPKLQEE
                     ILAVIAKYVNISRDQVSVNLERMEDSAVLELNITMPDKALEDSNS"
     gene            complement(9996..12482)
                     /locus_tag="ACF2SP_001105"
     CDS             complement(9996..12482)
                     /locus_tag="ACF2SP_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFD2358899.1"
                     /translation="MPELILILAAIGVAGTLLTRHASISVWAISYTIFLGLVLQFSSP
                     GLLSQIVLWAIEAVLVLGSIKPLRREVLSKHLFQIIKKAMPSMSITEREALEAGTVSW
                     EGDLFSGNPNFSLLRNAPTVALTEEEQAFIDGPVSALCEMLDDWDITHNRTDLPPEVW
                     QFIKENRFLGMIIPKQYGGLGFSATAQMSILVKIYGRSITAATTISVPNSLGPGELLL
                     KYGTEEQKNYYLPRLADGREIPCFALTGPNAGSDAASIPDKGVVCRQEFNGELILGVR
                     LNWDKRYITLCPVATVIGLAFRMFDPDNLLGKGEDIGITCALIPATTPGITKGRRHFP
                     LNTGFMNGPTQGKDVFIPLDYLIGGASMAGQGWRMLMECLSAGRAISLPSSASGGAQA
                     VALASGAYARIRKQFNQPIGKFEGIEEPLARIASAAYIIDSSLTMAAAAIDNGAKPSV
                     AGAILKYHTTERARQLALDGMDIHGGKGICLGPNNYIGRGYQGSPIGITVEGANILTR
                     SLIIFGQGAIRCHPYVFKELESIRNNDLVAFDDSFFAHVGFVITNFTKSLIFGWTDAR
                     FSKSPASSVKRYYQLIHKYSSNLAFLADFSMIVLGGELKRKEKLSARLGDMLSCLYMA
                     SAVLKRFYEDGEPRADLPVVHWSCQQLLHECETAMHGVIINFPARWARIVLSLILKPL
                     GNKRHKPDDKLGHKVARILIEPNETRNRITRLVYKKPGDNCPLGRMEEAFHKICAVEE
                     LERKVMKAVKDNILHSLTFQEQILEALEHQILTPVEAKQLEEAEKARQDVIRVDDFAD
                     EELRRPSSLGEKVKSEKKIPARDDMETETA"
     gene            complement(12575..13360)
                     /locus_tag="ACF2SP_001106"
     CDS             complement(12575..13360)
                     /locus_tag="ACF2SP_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2358900.1"
                     /translation="MRELIHFAHGNGFPSLCYKQLLEQLETRFDCCFIDKIGHDPDYP
                     VGENWHNLVAEIIASIKRQAEQPVIAVGHSLGGVLSLLAAIEQPKLFKAVIMLDSPLI
                     GAFKSSMVRLAKALGIIDRVTPAFRTKKRREHWQNYEQLISYLKTRDLFKTFTEECLN
                     DYIKYGLQYKEDGYYLRFDRHVEYQIYRTIPHVIPRYKGKLIVPAALIYGDKSTVVDR
                     IDIRYMKNHFNVKCFKTMGTHLFPMEHPKAVGKQVIEVIDAII"
     gene            complement(13357..14274)
                     /locus_tag="ACF2SP_001107"
     CDS             complement(13357..14274)
                     /locus_tag="ACF2SP_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HFD2358901.1"
                     /translation="MYLMREQENVWESGVAKEKNKLKPIDAPIYSYWQALYMSFYSKR
                     LFVDVGKHWRGIGLIYLLLVIAICSIPFSIRIASDFNKTFNQQIIEPLLQLPTVYVQN
                     GNVIFDKPMPYLIRNDKNQVVIIIDTTGKVADFTEEYPYLNILIKKNSINFLLPTPEL
                     LGSSPVNEQKGVPIVQPLDKDMNMVFDGKKIIQDNAINGLKYASQLMIYPIVVAILYS
                     IMVTVLLVIAFLGQVFSKIFFSFTISFKKSSRLLMVASTPMLVVLVTMLSLHFIFPGF
                     GVILLILLAAYYSYGVYGLRDESRKMVLK"
     gene            complement(14225..15316)
                     /locus_tag="ACF2SP_001108"
     CDS             complement(14225..15316)
                     /locus_tag="ACF2SP_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2358902.1"
                     /translation="MATLNLIEVSKQVGETTILNRINVDIKKGEFMVVVGPSGCGKST
                     LLRLIAGLDVLSSGKILINNQCVNDTEAAKRDMAMVFQNYALYPHMTVFDNMAYGLKL
                     RRFSKADINQRVTGVAKLLHLTPYLDRKPQALSGGQKQRVAMGRAIVRSPAVYLFDEP
                     LSNLDAKLRTEMRHEIKRLHQQFNTTSLYVTHDQTEAMTMADRILVLNQGEVEQIGTP
                     QELYQSPATQFVAGFTGHYPINFISGKYDKKTDSIHTDIGIVYSAPNWLHSVDDKADL
                     IVAIRPEHIQLCSSQNQDAVELHVEFVDDMGADKLIQAKCVKNNEQINLRISAEQMVS
                     NRQLHVELPKLKLHVFDERTGKRVGEWRG"
     gene            complement(15316..16143)
                     /gene="ugpE"
                     /locus_tag="ACF2SP_001109"
     CDS             complement(15316..16143)
                     /gene="ugpE"
                     /locus_tag="ACF2SP_001109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFD2358903.1"
                     /translation="MRQFKRFGSHGLILLFIGLMMAPLYLAVVAASHEGTVMMHSQLP
                     LIPGTSFFKNIKSVLTEGLAVTGGEPITSMLVNSLFMAFIIAVGKIILALGSAFALVY
                     FDFPFKKSCFALIFATMMLPVEVRIVPTFQVVASFGLLNSFSGLTLPLLASATGTFLF
                     RQFFKTISKELVDAAKLDGAGPLRFFIDIVLPLSKIQISALFVILFVYGWNQYLWPLV
                     ITTETKMTTIVMGIRYLAGVADQVPEWHYIMAVALIALVPPCMVVMAMQRWFEKGLK"
     gene            complement(16140..17018)
                     /locus_tag="ACF2SP_001110"
     CDS             complement(16140..17018)
                     /locus_tag="ACF2SP_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFD2358904.1"
                     /translation="MPKFNHQRLFAWLFIVPQLIVTIIFFIWPACSALLQSFFYTDAF
                     GLHKNFAGLTNFFDLFLDPGYAKAIWVTFVIAISVTLITMSLGLLMATLINYREKSQG
                     VYKTLLIWPYAIAPAVAAILWRFLCHPTLGWLTQVLEFLGIHFNYVNDANQALLVVIL
                     TASWQQFSYNFLFYFAALKAVPQTLIDAAIMDGANAWQRFWQIIFPILSPTTFFLLIM
                     NLLYGFFDTFGIIQVMTHGGPGNSTTNLIYKVYEDGFEGMDLGSSSAQSVLLMLIVIA
                     VSLLQFKYLEKKVHYE"
     gene            17168..18139
                     /locus_tag="ACF2SP_001111"
     CDS             17168..18139
                     /locus_tag="ACF2SP_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFD2358905.1"
                     /translation="MHYIQINNPGPHSELTLVEGPNPGFNESQILVRVKATALNRADL
                     MQKYGKYPPPPGESDIPGLEVAGEVIITGSKVKQFKPGDKIYGLVGSGGYAEICPVDA
                     SLAHHIPSHWNYSLAAALPEALTTVYATLYDLGALSAGQTLLIHGAGSGIASLAIQMA
                     KLTTTKVITTVGDSSKIEKALALGADQVIHYKEQDFELLIEDNSVDLIIDFVGGDYFN
                     KHLHLLKPQGKLIQIACLKGSKVECSLALLMQKRLHIIGFVLRSQSIKEKARLWKLAH
                     DHWFNAFENDKIKPVIDSEFDLNQIETALQRMQQGSHFGKIVIRVGK"
     gene            complement(18313..19596)
                     /gene="clcA"
                     /locus_tag="ACF2SP_001112"
     CDS             complement(18313..19596)
                     /gene="clcA"
                     /locus_tag="ACF2SP_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFD2358906.1"
                     /translation="MGKKILILYFVSVLLGILTGAIASLFQLTIQQMDHLLELLFHIT
                     ESYGLPVGIVSAMISMMMLFIAWMMVKYIAPEAAGSGVQEIEGTLLHVRPIFWKRLIP
                     VKFFGGVLSISAKMVMGREGPTIQMGGNLGQMLGELFRFTRRRRDTLIAAGSAAGLAA
                     AFNAPLAGVLFVMEEMRNQFNYSFTNFKMVVICCVMATIVLHLIIGSQPAIQMEVFEL
                     PTLGSLWLFFVFGILMGLAGLLFNVTLMRTVGWLDKQNPKNKKYYILAVGFLVGYLAY
                     SYPATVGGGYVIISEALTMSPAFSVLCSLIVIRFIMTMLSYSSGVPGGIFAPMLALGT
                     LFGLGFFHLFQWLSMDFSIEPGMFAVAGMGALFAAAVRSPITGVVLVVEMTKNYSLIL
                     PLMVSCITATIVVQLARNEPIYTQLLERTLRLNAK"
     gene            complement(19589..21736)
                     /locus_tag="ACF2SP_001113"
     CDS             complement(19589..21736)
                     /locus_tag="ACF2SP_001113"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFD2358907.1"
                     /translation="MLQQYKTLGGVHVEFSQQELNYQQGIDFLLESLDSQRGAVFASS
                     FEYPGRYTCWDMGFYNPPLVMICQSNTICFEALNQRGEILLSFLYPLLKTTDLWEVIS
                     QTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
                     YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREG
                     KYSPYQPIHKPEKNCDHEPGEYAEVVKKAKEKFSCGDLFEVVPSQTFYTYFAEKPSIL
                     FRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIED
                     AHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHV
                     EGILRDGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGN
                     LNTGLVLRTVRIEKGTAEIRVGATLLYDSIPEAEEEETRLKASAFLDILQKPWQKAKK
                     KTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQNNHFDLI
                     VLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSS
                     VIRVLDNKGLFSGLGDCFKAGRYHSLYARAETLPNELLVTAVSEDDVIMAVSHEHLPV
                     HAVQFHPETILSLSDQVGLRIIINLMNMVKGNG"
     gene            22010..22306
                     /gene="gatC"
                     /locus_tag="ACF2SP_001114"
     CDS             22010..22306
                     /gene="gatC"
                     /locus_tag="ACF2SP_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFD2358908.1"
                     /translation="MTLSAKDLENISKLAYLNEDSEHSAKLTQEVSAIMDFVDQLKTI
                     DTSQIAPLFHPLALNQRLRPDEVTEAECLAELEAMAPLFEDNLYLVPKVISSDK"
     gene            22309..23760
                     /gene="gatA"
                     /locus_tag="ACF2SP_001115"
     CDS             22309..23760
                     /gene="gatA"
                     /locus_tag="ACF2SP_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFD2358909.1"
                     /translation="MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLD
                     EDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDAT
                     IVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
                     NLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAED
                     LAMILHCIAGFDSKDSTSVDRVIPDYSAEIKKPVDKIRIGLPSCFFQPQVEKGIQDAI
                     HNAVKLFENLGAEIIEIDLKLQPFWVPCYYVIACAEASSNLSRYDGIRFGHRSKSAST
                     LIELITNSRSEGFGNEVKRRILTGTHVLSSGFFDAYYLHAQKVRRLIRDELITTLNSV
                     DVILGPTTPTTAFKLGEKINDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQ
                     LMGKHFSESRLLAIAHHYQQHTNWHLANPNKQG"
     gene            23764..25197
                     /gene="gatB"
                     /locus_tag="ACF2SP_001116"
     CDS             23764..25197
                     /gene="gatB"
                     /locus_tag="ACF2SP_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFD2358910.1"
                     /translation="MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGL
                     PGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTN
                     GYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
                     YSAEEAVNYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLN
                     SFRFIEKAIAFEQARHQDILERGLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDP
                     DLLPIDIDKEQIEEIKNNLPDLPEAIYKELKNTPSLNDEDINFILSSPDTYQYYKKIK
                     SLCPAADKTIINWLKGQYAAFLNEHNLTFETPPISAKTMAAFLSKIHEKKISSSIAKN
                     IFSMLCAGEEDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPEQVIEYKAGKEKLLAF
                     FIGQAMKQTKGKANPEQINLLLKKHLG"
BASE COUNT         8120 a         5273 c         4381 g         7513 t
ORIGIN      
        1 tgttataaca atgcatgacg atatcgaaat cattctcatc gatgtatgta aagtattcat
       61 tgatatcaat aggattttgt cctattaaaa attttacaac agattgaaaa tcaaggttat
      121 tcagtactaa tgcggcatta tgtaaaaaag tcttgtcaga tcggtcatgc actaaaagat
      181 taacctgatt tttaatcaat agttgaatga tatcgatgta ttttttggta tccttcgcta
      241 aagaataccc tttcaatgtc aaactcaaag gacaatgacc agaatgactt tgttgttgat
      301 cgatattagc gccatgttca ataagccatt ttacattttc aacttgtcca agataacagg
      361 cagccatcaa aggggtgaat gtatccgcct catcaaaatc taatattttt tcgtcagaac
      421 ctacttttaa aaatggcaaa tcgatatctg aggcatttaa ttcttgttct tttgcaaggg
      481 aatagttagc gaaaattatc cgtcctattt gagtcattaa aattctatct cctgctttaa
      541 gatcaaatcc ttccaaatta tagggcattt gttctaatcg gtactgagat tggtaagttt
      601 gataaaattc ttgtgccgat agccaaccat cgccaggatt aagaaagtag taatacctgg
      661 aagcttgctc ttcgtaggta ttatagttga ttaatagttg ttgtcctgcc ttgatgtttt
      721 tagtggttat aacttttact actttcttgc gattcaatgt agtttcaaca aactctgcat
      781 tatcctggct atccgaaaaa ttgatataac gagtcaaatt accttttctt gctgcatcaa
      841 ctacccgtcc cccaacatac atcgcgtaac ttttatcaga accaacatgc tccattagat
      901 attgttcaaa ttcttgttca gaatacactt ctcctgtgta aataccaata caagtcccct
      961 ttgggatatc ttctctggca aataaacctc gcccaccggt ttttctgagt aaatggacag
     1021 gctgaacaat aacaagatca gttctgtcta tagcagatgg aatgtaatca ctgcttgaca
     1081 tgttatcaat atgcaaatta accataattt tgctcgtgtc agtcaattga gcattgtttt
     1141 tcaatccatt gacgggggta aacagtgtgt aaatattacc tgggaggtgt gtcatcccag
     1201 gtcctttttt ctttgccttt ctttgggtaa atttaaattt agacttgtgc tctttgctac
     1261 catgattcca ttgctgttct ttaaactttg actgaagggc acttttcttt ttaaggttgc
     1321 tatcattctt gcttcttggc ataacactac tctcttgtaa aaaatcatat aaaataatca
     1381 ttaaggagcc attatactct attagagtat tttttgctct attttgcatg tgattttcat
     1441 tgaaaatcta ttattttgct gattgaagag tattgttgct cctcttaccc atgtgctaac
     1501 atttatgatt ttggataaac cataatgggc gttacaatta aaactcctga tgaaattgaa
     1561 aaaatgcgtg tcgctggcag actggcagcc gaagtacttg agatgattgg gccttatgtt
     1621 caagttggca ttacaactga tgaattaaac actatttgcc acgattatat tgttaatgta
     1681 caaaaggcta ttcccgcccc tttaaattat aacggtttcc ccaaatcaat ttgcacctca
     1741 attaatcatg tggtatgtca tggaatacca gggaaaaaaa ctttaaaaga tggagacatc
     1801 atcaatattg atgtcactgt tataaaaaat gaataccatg gtgataccag taaaatgttt
     1861 attgtaggca ccccttctgt caaggccaag catgtagtgc agatagcaca tgaatgcttg
     1921 tatattggaa ttgatatggt taaacctggt gtccagctag gcgatatagg gcatgccatt
     1981 cagcaacatg cagaaaaaaa tcgttgctct gtggtgcgcg attactgcgg acatggaatt
     2041 ggccgtgtat ttcatgagga tccacaagtg cttcactatg gagttcccgg aacagggatg
     2101 aaattagaac cagggatgac ttttactata gagccgatgg ttaatatagg gaaacatcat
     2161 actcggttat taccagatca ttggactgtc gttaccaaag atcatagtct ttcagctcaa
     2221 tgggagcata cacttttggt gactgataca ggtgttgaaa ttctgacttt acgtaatgaa
     2281 gaacgataat cgcatcataa aaaatacgat taaacagttc aaggagaagc tgtgcaagga
     2341 ctttagccaa aaagctaata ttacctccat cacacgcaag cttgctgtat ttattgatac
     2401 tattttaatt caattattta ttaaaaataa gttacacttt ggagataatt tttgtctcct
     2461 ggctcttgga agctacgggc gtagagagct tcaactccac tcagatattg acttgttaat
     2521 attgcacact gaaaaagtaa gcaatatcca attacaacgc gcacaaaaat ttatccagga
     2581 ttgttgggat gtaggactgg aggtaagcca tcaaattacc actgtttctt catgtgccaa
     2641 tttggccagt caggatttga gcgtaatttc taccattatg gatatgttct tactgtgtgg
     2701 gcatggtgcc ttaatggagg aattaatata ccaaacacac accttgcaca tgtggcctag
     2761 ccatcaatac ttctttgcca aacttcagga acagcaaaat cgttatgcaa agtatggaga
     2821 aacagcctac aaccttgaac ctaatattaa aaatggtcca ggtggattaa gagatctgca
     2881 aattttgcta agcatcagca aacgccattt taaaattaaa aaattagccg agggaatcgg
     2941 ttacggcttt attactgata aagagtatga agaattaaaa tactgccaga actttctttg
     3001 gcgcgttcga tttgctttgc atatgttagc aggaaagcct gaggagcgct tgtcatttga
     3061 ttatcaggtt aaattagctc aattttttgg atatcaagat cagtctcata tcttggccat
     3121 agagcaattt atgaaagact actttaaagt catcaaacgc aatagagagc tgaatgaaat
     3181 gctcttgcaa tggttcaatg aaaccatagt ctatcatcaa aagcaaaaaa ttattcgtct
     3241 ggatgatgaa tttcaactgt caaaccgatt cattgaagta cgcaataacc gtgtatttaa
     3301 acaaaatccg caatctatat tgaaactatt ctattggctt gttaaacgcc ctgacatcga
     3361 aggagtaagg gcaagcacaa tcagactaat aagagaatca ttatttttaa tgggcaaacg
     3421 ctttagagaa tcaaaagaaa ccgctaatat atttatcaac atattcagaa ctggtaatga
     3481 tccatacgat gcattacaaa gaatgaatag atatggtgtt ctagcgcact atcttgattg
     3541 ttttgctaca gtcaccggac aaatgcagta tgatttattc catgcctata ctgttgatca
     3601 gcatacactg tttgtgatcc gtaatatatc tcgctttaaa aagaacgaat atgccaaaca
     3661 atttccttta tgcgccaaaa tcatctctgc tttagagaaa cccgaaattt tgtatctcgg
     3721 cgctttattc catgacattg ccaagggtcg gggaggagat cattctgaat taggagcaat
     3781 tgaggcacaa caatttacac aacgccacta tatggaggca gaagatagca aattgattgt
     3841 atggcttgtt cgctaccatt tactcatgtc acaaactgct cagcgtaaag atatttatga
     3901 tcctaaaacc attgagcaat tctgccaact tctgcctcat gcaaggtatt tggattatct
     3961 ttatttactc acagtagcag atatttgtgg tacaaatcct actctttgga atgcctggaa
     4021 agattcattg cttaaagaat tatatcatgc agcaaaaaca agacttcata aacaacaaga
     4081 gttgcttgat gaagcagctc tgatatccat tcgtaaacaa tacgctatgg atatattaat
     4141 ttcagatggg atatcctcca gagtcataca ggatttatgg agccaattta aggggaaata
     4201 ttttcttcat gaatctcctg aggtgatagc cagacacacc aaggccatac tgaacagcaa
     4261 acaatttcca gtggtaataa ttatgccgca tcacagtcag ggtggaaccg aagtatttat
     4321 ctacatgcca cataaagatg agagatttac tatcaccact tcagtattaa gcaatcatca
     4381 cgttactata caggaagcag caattattac ttgtgataat caatttgatc tggatactta
     4441 tattatttta gatgaaaaca atcaggcttt tttgaatgaa caacgtgcca gagacatcca
     4501 aaaaagtcta tgcgatcatc tggccaatac aggacgcctc ccggctgttt ctcgcagaag
     4561 attatccaga gcattgactc attttaatgt caaaacacaa attaacttta ttgatgataa
     4621 tacgaatcat caaacccaac tatttctcgt taccaatgac aggcctggac ttttggcgac
     4681 tataagccgt gtgtttctta cactaaacat tcatttacat aatgcaaaaa ttgctacggc
     4741 tggtgagcgg gtagaagata tgttttacat ctccaaccag acaggttatt ccctgaatca
     4801 tgaggaaaaa acaattttaa aagaaaaatt aattttggaa atttctaaaa gcaaatatta
     4861 agaaatatgc caagataaat tactagcgaa ggtttctgaa aaaatctgat agcaattgag
     4921 aacactcagc ctgcataatg ccttcgtcta ttaataccga atggtttaga gcatgtaaaa
     4981 cattaaacat tgagcccgct gcccctgctt taaaatctcg ggtggcaaat accagccgtt
     5041 tgatgcgtgc atgaaccata agtcccgcac acatcacaca gggttcgaga gtaacataaa
     5101 gcgttgtatc caataatcta tgattattta gctttctcga ggcttgtcgt attgccctga
     5161 tttcagcatg atcagaagga tcatgacttt tttcaatgac atttctgcca acgcctaata
     5221 attgattgtc tttgctaacc aacacagccc caacaggaac ctctccttca ttacgagcta
     5281 taaccgcttg ctcgtaagcc agttgcatcc aaaatttatc aatcattccc attcaatggt
     5341 agcaggtggc ttgccagaaa tatcgtaagt gacacgtgat actccctcta cttcattaat
     5401 aattcgattg gatacttttc ctaaaaaatc ccagggcaat tgagaccaat gtgctgtcat
     5461 aaaatcaacg gtctcaacag ctcttaaaca aattacgtaa tcatatcggc gcccatcccc
     5521 catcacaccc acgctcttta ctggtaaaaa aacagcaaat gcctggctga ttttatgata
     5581 aagctgcgcg ttatgtagtt cttcaataaa aatagcatcg gctttacgta atatgtccgc
     5641 atattctttt tttacttccg ctaaaattct tacacccaat ccaggtccag gaaagggatg
     5701 tcgatagacc atatcatgcg ggagtcccaa ttccagacct acatgacgta cttcatcttt
     5761 aaagagctca cgaataggct ccagcagttt aagatttaat gtatctggta agccaccgac
     5821 attatgatga gactttatga ccacagccgc atcattagta cttgttgctg cagactcgat
     5881 gacatcgggg tagattgtgc cctgagccag ccactttata tcagtcagct tctgtgcttc
     5941 ctcatcaaag acttcgataa atgtacgccc aataattttt ctcttttcct ctgggcaagt
     6001 tactcccttc aaagcagtta aaaatttatc ttcggcattg actgcaataa tgcgtattcc
     6061 catatgccga ccgaacatgc tcagcacttg ctccgcctca ttaagtctga gtaaacccgt
     6121 atcaacgaaa acacaaacca gttgctctcc aatggctcta tgaagtaatg cggctacaac
     6181 agaagagtca acgcctccgg ataggcccag taaaactttt tcagttccta cctgttctct
     6241 gattttatta atggcttcat caataatatg ctctggagtc cagtctgttg atgctttaca
     6301 aatatctacg acaaaacgtt gtaaaattct taaaccttgc aaggtatgag taacttctgg
     6361 atgaaattgc aaaccataca tttgacgact ttcatcagcc atacctgcaa ttggtgcatt
     6421 acgtgtttca catatcactt taaaacctgg tggtaactca gtgactttat caccatggct
     6481 catccaaaca tccagcagag cgctgccatc catcgcggta cgatcttcga tattacttaa
     6541 taattgacta tggccatgca aacgcaactc agcatatcca aattctctta gcgttgatga
     6601 atgcacttga ccaccgagtt gtacagccat ggtttgcatc ccatagcata tacccagcaa
     6661 tggcaaatcg ctctcaaaca gccattgagg agccctggga ttggcatcat gcgttactgt
     6721 cgaaggacct cctgataaaa tcacaccaca agggtttaat tttttaaatt gttcatgatt
     6781 aatattataa ggataaattt cacaataaac ccccatttct cgaacgcgtc ttgcaattaa
     6841 ttgggtgtat tgagaaccaa aatccaatat taataatggg ctttttttca aatcattcat
     6901 tattaattat ccacctgata attgggggct tgcttagtaa tactcacatc atgcacatgt
     6961 gactcacgca taccagcatt ggtcacttgc acaaattcag ttttagaatg caattcctca
     7021 atagtagcac aaccggtata gcccatacag gagcgtaaac cacctaataa ttgatgaata
     7081 attgtttgaa ccggtccttt ataaggtacc cttccctcaa taccttcagg aaccagcttt
     7141 tctgtaccaa gagaagcatc ctgaaaatag cggtcacttg acccttgagc taatgacata
     7201 gctccaatgg atcccatacc tctgtaattt ttataagtac gcccctgata taattcaatt
     7261 tcgccaggag attcttccgt acctgcaaac atactaccaa gcattacagt atcagcacct
     7321 gcggccaacg ctttacaaac gtcacctgaa aaacgtatcc cgccatcagc tatcacagga
     7381 attatccctt ttagttcttg tgccacattt gctatagcgc taatttgcgg gataccaaca
     7441 ccggtcacta tgcgagtggt acaaattgat cctggcccaa ttccaacttt tactgcatca
     7501 gcacccgcct catataaatc tcttgcggcg gcagcggttg caatattgcc acctatgact
     7561 tgtacatcag gataattctt tttaatccat ttcacacgat ttaatacgcc ctgagaatga
     7621 ccatgcgctg tatccactac tatgacatca actccggatt caaccagagc tgcaactcgc
     7681 tcatcggttc cttcaccaac accaacagca gccccaactc ttagctgctc aaaactgtct
     7741 ttacaagcat aagggttgtc ttttgctttt tgtatgtctt ttactgtaat gagtccacgt
     7801 aactcaaaat tctcattcac taccaataat ttttctattc ggtgcttatg gagcaaactt
     7861 ctgacttctt ctctactcgc tccttccttt acagtaacca aacgcccttt gggagtcatt
     7921 acttgcgcca cagtcaaatt catattggtt tcaaaacgaa tatctctact ggttactatt
     7981 cctaccaaat gcttaccatc aaccaccggc acccctgaaa aattatattt tgtcatgact
     8041 gcaagcaatt ctttaactgt caagtcggga gtgactgaaa taggatcttt caccatacca
     8101 ctttcaaatt tttttactct tctgacttcc tctgcttgat cagcgatact catattctta
     8161 tgaataatcc ccaaacctcc ctcctgtgct aaagcgatag ctaacctcgc ttcggtcact
     8221 gtatccattg ccgccgaaat taaaggcata ttaaggtgga ttgcacgggt taatttggtt
     8281 ttcaatgaaa cgtctttggg taaaatagtt gagtgggcag ggacaagcaa aacatcgtca
     8341 aaggttaatg actgttgcac aatggataga ggcattttta tctcactcca gtaattaaat
     8401 aaccaaatag tttactacaa actttaaaaa atttaaacat ttattgtata aatatatcac
     8461 tacaatcttt taattagttt ttataacaat taagctttgg ataacttgaa ttaatgaatg
     8521 aataacgtgt aaactgttgg ttaaatattg tgttgaatcg aaaaaaatag gaagggttca
     8581 gcgttggcta aaataatagt aattacatct gggaaaggcg gagtaggtaa aactacttca
     8641 tcagcagcga tttcttcggg acttgcactc ttagggcata aaactgttgt tattgatttt
     8701 gatatcggtt taagaaacct agatatcatt atgggctgcg aaagacgtgt agtgtatgac
     8761 ttcatcaacg tcatcaatgg tgaagccaac ttaaaccaag ctctcatcaa agacaaacgg
     8821 atccctaacc tttgcatact gcccgcatca caaactcgtg ataaagacgc actaactctg
     8881 gaaggagtag aaaaaactct caatgagcta gcaaaagatt ttgatttcat tatttgtgac
     8941 tcccctgctg gaattgaaac tggagcactt atggcaatgt attttgctga tcatgccatt
     9001 gttgttacta atcctgaagt ctcttctgtt agagattcag atagaattct gggaatactg
     9061 gccagcaaaa caaaaagagc tatagaaaat aaagcacctg ttcaagaaca cttgttattg
     9121 acacgatatg atcctgagcg agtagaacga ggtgaaatgt tgtctgtaac tgatgtaaag
     9181 gaaatattag ctattccttt aattggtgtt atacctgagt ccaaatcggt tcttaaagca
     9241 tccaatactg gtacgccagt tattctggat gaatcaagcg atgcgggcat tgcttatcaa
     9301 gacgccatag cacgcttttt gggcgaagaa agaccaatgc gatttataag tattgataaa
     9361 aaaggactat ttcgtcgctt attcagtaaa aacaaggagg aagtgacaat atgagtatct
     9421 tcaattactt acgcagaaga gcctctaccg cttctgttgc taaagagcgc ttacaaatca
     9481 tcatttctca tgagcgttct caacgcaata caccagacta tttgcctaaa ctgcaagaag
     9541 aaattcttgc agttatagca aaatacgtaa atataagtcg agatcaagtg agtgttaatc
     9601 tggagcgcat ggaagatagt gccgttcttg agctaaacat tacaatgcca gataaagcat
     9661 tggaagacag caactcataa ttctattaag ttttttatac ttataggtat tattacacta
     9721 tcaaataatg tagtttacct acctacttaa catacgcatt aaaacattat ttcctccaag
     9781 aatatttcaa aaaactttaa ataaaatacc taaatgagtt aagcattaca tagatgggag
     9841 ttaaagtcat tcttaatggc tctgtctgta aagaacaatt gattaaaacc aaaattaata
     9901 aaaacatatg ggaatgatta gcctgactct aattactgaa tagggttaca tcatgtaact
     9961 ctattcagca ttatagaaaa atatctagta taaaattaag ctgtttcagt ttccatgtca
    10021 tccctggctg gaatcttttt ttctgacttt accttctcac caagtgacga gggacgacgt
    10081 agttcttcat cagcaaaatc gtctaccctt ataacatcct gtctggcctt ctcagcctcc
    10141 tctagttgct tcgcttcaac tggagtcaat atttgatgct caagcgcttc caatatttgc
    10201 tcctggaaag tcaatgaatg aaggatattg tctttaacag ccttcatcac tttgcgctct
    10261 agctcttcaa ctgcacaaat cttatgaaat gcttcctcca tccttcctaa gggacaatta
    10321 tcccctggct tcttataaac caatcttgtg attcgatttc ttgtttcatt aggctctatc
    10381 agtattcttg ctaccttatg acccaattta tcatccggtt tatgcctttt gttgccaaga
    10441 ggttttagaa ttaacgataa aacaattctt gcccaacgtg ctggaaaatt aatgattacc
    10501 ccatgcatag ctgtttcgca ttcatgcaat aactgctgac aactccaatg aacaacaggt
    10561 aaatcagcac gaggttcccc gtcttcgtaa aatcgtttta aaacagcgga tgccatgtac
    10621 aaacaactca gcatgtcacc taagcgtgcc gataattttt cttttcgctt taactcacca
    10681 cctaaaacaa tcattgaaaa atctgctaaa aaagccagat tagaactgta cttatgaatt
    10741 aattgataat acctttttac agaactggct ggagacttgc taaaacgagc atcggtccat
    10801 ccaaaaataa gtgactttgt aaaattagta ataacaaaac ccacatgggc aaaaaaggaa
    10861 tcatcaaacg caaccaaatc attatttcta atactttcta actccttgaa aacataagga
    10921 tgacaacgta tcgctccttg cccgaatata attaagcttc tcgttaaaat attggcccct
    10981 tccaccgtaa tgccaatagg agaaccttga tatcctcttc caatataatt atttggacca
    11041 aggcatatcc ctttaccacc atgtatatcc attccatcca gtgcaagttg tctcgctctt
    11101 tcagtagtat gatactttaa aatagcccct gcgacagaag gttttgctcc attatctata
    11161 gccgctgctg ccatagttaa agaagaatca atgatataag cagctgaggc tattctggca
    11221 agaggttctt ctatcccctc gaacttgccg attggttgat tgaattgttt acggattctg
    11281 gcataggcgc cagatgctaa tgcaacggct tgtgcacctc cactggcgct tgatggtaaa
    11341 gagattgcac gacccgcgct taaacactcc attaacatcc gccacccttg accagccata
    11401 gaagcaccac cgatcaaata atcaagcgga ataaaaacat ctttgccttg cgttggacca
    11461 ttcataaacc ctgtatttaa agggaagtgc ctgcgccctt ttgtgatccc cggagtagtt
    11521 gcaggaataa gcgcacaagt aataccaatg tcttcacctt tgcctagcaa gttgtccgga
    11581 tcaaacattc taaaagccaa accaataact gtcgccaccg ggcacaaggt aatatatcgt
    11641 ttgtcccaat tcaaacgaac tcccaatatg agctcaccat tgaactcctg ccggcaaaca
    11701 acacctttat caggtataga agccgcatcc gaaccagcat taggtcctgt caaggcaaag
    11761 catgggatct ccctcccatc ggccaatctt ggtaaataat aatttttttg ttcttctgtc
    11821 ccatacttta aaagtaattc accaggcccc agtgaattag gtactgaaat agttgtagca
    11881 gcagtaatag aacggccata gatttttacc aaaattgaca tctgagcggt agcggaaaaa
    11941 ccaagtccac catactgctt tggaattatc attcccagaa aacgattctc ttttataaat
    12001 tgccaaactt cggggggaag atccgttcta ttatgagtga tatcccaatc atctaacatt
    12061 tcacagaggg cactgacagg gccgtctata aaagcctgct cttcttcagt taatgcaaca
    12121 gttggagcat tcctcaaaag agagaaatta ggattaccac taaataaatc tccttcccaa
    12181 ctcactgtac ctgcttccaa tgcctctctc tcagtaatag acatggaagg catcgctttt
    12241 ttaattattt gaaacaaatg tttcgatagt acttctcttc ttaaaggttt aatggagcct
    12301 aaaactagta cagcttcgat tgcccataag acgatttgcg acaacaagcc aggagagctg
    12361 aattgcaaaa ccaagccaag aaaaatggta taactaatag cccatacaga tatcgatgca
    12421 tgtcgggtta ataacgttcc agccacacca attgctgcta gtattaatat aagttctggc
    12481 acaacattct ccttattata ggctaatcca aatgaaagaa tttaatagac ttctttctcg
    12541 ataacaaaat ctgcaaacca gttaagacgt ctaatcaaat tatagcatca ataacttcta
    12601 taacttgctt tccaacagct ttagggtgct ccattggaaa taaatgtgta cccatagttt
    12661 taaaacattt tacattaaaa tgatttttca tatatcgaat gtctattcta tcaactacag
    12721 tactcttgtc accatagatc aatgcggcgg gaactataag tttaccttta tagcgaggaa
    12781 taacatgcgg aattgttcta tatatttgat attcaacatg cctgtcaaaa cgcagatagt
    12841 atccgtcctc tttatattgc aaaccatatt taatatagtc attcaagcat tcttctgtaa
    12901 atgttttaaa taaatctctg gtttttaaat aggaaattaa ttgttcatag ttttgccaat
    12961 gctcacgcct tttctttgtt ctaaaagcag gggttactct gtctatgata cctaaagcct
    13021 tagccaatct caccatgctt gatttaaaag caccaatgag aggcgagtct agcataatga
    13081 ccgctttaaa taatttcggc tgctctatcg cagcgagcaa actcaaaacc cctcccaaag
    13141 aatgaccaac tgcaatcact ggctgctctg cttgtcgttt aatgcttgca atgatctcag
    13201 caaccaggtt atgccagttt tctccgacag gataatcagg atcatgacca attttatcta
    13261 taaaacaaca gtcaaaacga gtttccagct gctctaataa ctgtttgtaa cataatgatg
    13321 gaaatccatt gccatgagca aaatggatta actctctcat ttgagcacca ttttcctgct
    13381 ttcatctcgg agaccataga caccgtaact ataataggct gccaataata tcaacaaaat
    13441 cactccaaat cctggaaaaa taaaatgaag tgataacatg gttaccaaaa ctactaacat
    13501 tggcgttgat gcgaccatta acagccggct ggatttttta aaggatatag tgaacgaaaa
    13561 aaagattttg gaaaatactt gtcctaaaaa cgcaatgacc aatagaacag tcaccataat
    13621 cgaatataag atagcaacca ctattggata tatcatcaat tgagaagcat atttcaaacc
    13681 attaatcgca ttatcctgaa taattttttt gccatcaaat accatattca tgtctttatc
    13741 taatggctga acaataggaa ctcctttctg ttcatttact gggctgctac ccaataattc
    13801 tggtgttggc aataagaaat taatactatt tttcttaatc agaatattaa ggtaaggata
    13861 ctcctctgtg aaatcagcca ctttacctgt tgtgtctata attattacca cttgattttt
    13921 atcatttctg attaaatagg gcattggttt atcaaaaata acattaccat tttgtacata
    13981 aacagttggc agttgcagca aaggttcaat aatttgctga ttaaacgttt tattaaaatc
    14041 agaagcaatt cgtatagaaa acggtattga acaaatagca ataaccaata atagataaat
    14101 caaaccaatc ccacgccaat gttttcctac atcaacaaaa agcctctttg aataaaaaga
    14161 catatacagt gcttgccaat aactatagat aggagcatca attggtttta gtttattttt
    14221 ttctttagcc acgccactct cccacacgtt ttcctgttct ctcatcaaat acatgtaatt
    14281 tcaatttggg tagctctaca tgtaattgcc tgtttgagac catctgctct gctgaaatcc
    14341 tcaaattaat ctgctcatta tttttcacac atttcgcctg aatcaattta tcagctccca
    14401 tatcatcaac aaattccaca tgaagttcaa ctgcatcttg attctgacta gaacatagtt
    14461 gaatgtgttc aggacgaatt gccacaataa ggtctgcttt gtcatcgaca gagtgaagcc
    14521 aattaggtgc tgaatacact atacctatgt cggtatgaat gctatctgtt ttcttatcgt
    14581 atttgccaga aataaaatta ataggatagt gcccggtaaa accagctaca aactgcgtag
    14641 ccggactttg atacagttct tgtggcgttc caatttgctc aacttccccc tgattaagca
    14701 ccagaattct atcagccata gtcatcgctt cggtttgatc atgagtaacg tataaactgg
    14761 tcgtattaaa ttgttgatgc aaccgtttaa tttcatgtcg catttcagtt cttaattttg
    14821 catccagatt tgatagtggt tcatcaaata aatacactgc tggagaccgc acaatagccc
    14881 tacccattgc tactctctgc ttttgcccac cagatagtgc ttgcggcttt ctgtccagat
    14941 atggagtcaa atgcaataat ttagctactc cagtcactct ttgattaatg tctgctttgc
    15001 tgaagcgcct caactttaaa ccataagcca tattatcaaa gacagtcata tgaggataaa
    15061 gggcataatt ttgaaacacc atcgccatgt ccctttttgc ggcttcggta tcattaacgc
    15121 attgattatt aataagtatt ttacctgagc ttaaaacatc aagacctgct atcaatcgta
    15181 acaaagtaga tttgccacaa cctgatgggc caacaacaac catgaattcg cccttcttta
    15241 tatcaacgtt aattcgattc aaaatggttg tttctccaac ctgttttgac acctcgatca
    15301 aattcagtgt tgccattatt tcaatccctt ttcgaaccaa cgttgcatcg ccataaccac
    15361 catacaaggc ggcacaagag ctatcaatgc gacggccata atatagtgcc actcaggtac
    15421 ttgatccgct acacctgcca aataccgaat tcccatcaca atagttgtca ttttagtttc
    15481 tgtcgtgatg accaaaggcc ataagtattg attccaccca tagacaaaca agattacaaa
    15541 caaggcggaa atttgaattt ttgacaaggg taacacgata tcaataaaaa atcttaatgg
    15601 acctgcgcca tctaatttag cagcatcaac cagttcttta gatatggttt taaaaaactg
    15661 acgaaataaa aatgttccag tagctgatgc caataatggc aaggttagac cagaaaatga
    15721 gttcaatagg ccaaatgaag caaccacttg aaaagtaggg actattctta cttccactgg
    15781 taacatcatc gttgcaaata tcagggcaaa acaagacttt ttaaaaggaa aatcaaaata
    15841 taccaatgca aaagcagatc ccagagccaa aatgatctta cccactgcaa tgataaatgc
    15901 catgaataaa ctatttacaa gcatggaagt gataggctca cccccagtga cagctaaacc
    15961 ttcagtcagt acacttttaa tatttttgaa aaatgaggtt cctggaatca atggcaactg
    16021 agaatgcatc atgaccgttc cttcatgact tgcagcaact acagccaaat acaaaggagc
    16081 catcattaat cctataaata acagtattaa cccatgcgac ccaaaacgtt taaattgtct
    16141 cattcgtaat gaaccttttt ctcaaggtat ttaaattgta acaaggaaac tgcaattaca
    16201 ataagcatca ataatacaga ttgggctgat gaacttccca aatccattcc ctcaaaaccg
    16261 tcttcataca ccttatagat taaattagtt gtgctattac caggacctcc gtgtgtcatg
    16321 acttgaataa ttccaaatgt atcaaaaaaa ccataaagca ggttcattat taacagaaaa
    16381 aaggtagtgg gcgacaggat tgggaaaatt atttgccaga atcgttgcca tgcattggcc
    16441 ccatccatga ttgctgcatc aattaatgtc tgaggaactg ctttaagcgc agcaaaataa
    16501 aataaaaaat tgtaactaaa ctgctgccaa ctggcggtta aaataacaac cagcaaagcc
    16561 tgatttgcat cgttgacata attaaaatga attcccaaaa actcaagtac ctgagtcaac
    16621 catcctaaag ttggatgaca taaaaatcgc caaagtattg cagcaacggc aggggctatt
    16681 gcataaggcc atatcaataa agtcttataa actccttggc ttttttctct gtaatttatt
    16741 aaagtggcca ttaaaagccc taaactcata gtaataagcg tcacactaat cgcaattaca
    16801 aaagtaaccc atatggcttt tgcatagccg ggatcaagga ataaatcaaa aaaattggtc
    16861 aatccggcaa aattcttatg caaaccaaat gcgtcagtat aaaaaaagga ttgcaataat
    16921 gcgctgcaag caggccaaat aaaaaaaata atagtgacaa ttagctgagg gactataaat
    16981 agccaggcaa acaaacgctg atgattgaat ttaggcatta actaaccatg tagttgttaa
    17041 aatgataatt atcatcgcaa tagtttaaaa taaaattata tcgattttaa atcattatgc
    17101 gagttgtaaa agatataata tagtaaatta tcatactatg cattcttacc ttaaaaagga
    17161 tcttatcttg cattacatac agattaataa tccaggacct catagtgaat taacccttgt
    17221 cgaaggtcca aatcctggtt ttaatgaatc tcaaattctt gtgcgcgtca aagccacagc
    17281 cttgaataga gctgatttga tgcaaaaata tggtaaatat ccccctcccc caggagaatc
    17341 cgatatacct ggactagaag tagctggaga agtaatcata actggttcca aggtaaaaca
    17401 atttaaaccg ggtgataaaa tttacggtct tgtcgggagt ggaggttatg ctgaaatctg
    17461 tccagtagac gcttctttag ctcatcatat tccttctcat tggaattatt cattagccgc
    17521 agcactccct gaagcattaa cgacagtcta tgctacgttg tacgatttag gtgccttaag
    17581 tgctggacaa acattattga tccatggtgc tggaagcgga atagcttcat tagccataca
    17641 aatggctaaa ttaacaacca ctaaggtgat aacgacagtc ggtgattcca gtaagataga
    17701 aaaggctctt gccctgggcg ctgatcaagt cattcattac aaagaacaag attttgaatt
    17761 attaattgag gacaattctg ttgatttgat catcgatttt gtcggtggtg attattttaa
    17821 caaacactta catttattga agcctcaggg taaattaatc caaattgctt gcctcaaagg
    17881 aagcaaagta gaatgcagtc tggcgttgct tatgcaaaaa aggttgcaca tcattggatt
    17941 tgtactcaga tcgcaaagca tcaaagaaaa agcaaggctt tggaaattgg ctcatgatca
    18001 ttggtttaat gcatttgaaa atgacaagat taaaccagta attgactcgg aatttgatct
    18061 aaatcaaata gaaacagcac tgcaacgcat gcaacaaggc tctcatttcg ggaaaatagt
    18121 cattcgcgta ggtaagtaat gctaagcctc atcaatttct catgagctca atattatacg
    18181 cacagactct tccaaagctg ccttggcaat gtccagtgag ttcgtgtttt tggtagataa
    18241 atcaagggct cttcttactt ttatccttat tccccatagc tgaactatca atacatattg
    18301 agatatttag actcattttg cattgagtcg taaagttcgc tctaataatt gagtgtaaat
    18361 cggctcgtta cgtgctaatt gcaccacaat cgttgcagta atgcaggaaa ccataagagg
    18421 tagaattaga gagtaatttt tagtcatttc aacaaccaat accacgccag taattggtga
    18481 tcttactgca gctgcgaaaa gcgctcccat acctgcgact gcaaacatac caggctctat
    18541 tgaaaaatcc atacttaacc attgaaataa atggaaaaaa cctaatccaa atagtgttcc
    18601 caaagccagc attggtgcaa agataccacc aggcacgcca cttgaatagg ataacatagt
    18661 catgataaac ctgattacaa taagactgca taacacgcta aatgcaggtg acatagtcag
    18721 tgcttcactg atgataacat aaccgccccc aacggtagca ggataggagt acgccagata
    18781 accgactaaa aatccaacag ctaaaatata atattttttg tttttggggt tttgtttatc
    18841 taaccatcca acagttctca tgagtgtgac attgaataat aagccagcca gccccattaa
    18901 tattccaaaa acaaagaaca gccacaaaga gcccagtgta ggtagttcaa aaacctccat
    18961 ttgaatagcc ggttgtgagc caataataag atgcaaaaca atcgttgcca taacacaaca
    19021 aataacgacc attttaaaat tggtaaatga ataattaaac tggttacgca tttcctccat
    19081 gacaaacagt acaccagcca gaggtgcatt aaatgctgca gccaaaccag cggcagaacc
    19141 agcggctatt aaagtatcac ggcgacggcg tgtaaaccga aataactctc caagcatctg
    19201 tccaaggtta ccgcccatct gaatagttgg cccttctctt cccataacca tttttgccga
    19261 tatggataac acacccccaa aaaattttac tggtatcaga cgcttccaaa atattggtct
    19321 cacatgaagc aaggtgcctt caatttcttg cacaccactt cctgctgctt ccggggcgat
    19381 gtacttcacc atcatccagg caataaataa catcatcatc gatatcatag cggaaacgat
    19441 tcctacagga agtccataac tttctgtgat atgaaataat agctccagca gatgatccat
    19501 ctgttgaata gtaagttgga ataatgaagc aattgcgcct gttaatatcc ctaataggac
    19561 tgatacaaaa tataaaataa gtattttttt acccattgcc cttcaccatg ttcatcaaat
    19621 tgatgattat tcttaaaccc acttgatccg ataaagacaa aattgtctct ggatgaaatt
    19681 gaaccgcatg cacaggtaaa tgctcgtgag aaactgccat aatcacatca tcttcactta
    19741 cagcggtaac taataattca ttgggtaagg tctctgctct ggcatagagt gaatggtatc
    19801 ttcccgcttt aaaacaatcg cctaaaccgg agaacaaccc cttgttatct aataccctga
    19861 ttactgaaga ttttccatgc atagggtatt caagaacatc caaaacccct ccaaaatact
    19921 caacaattcc ttgcagcccc aaacaaacgc cgaatattgg tatgttcttt gagagtatca
    19981 catcgattgt ttctgaaagc ttaaaatcaa ccggttttcc ggggccaggt gataatacga
    20041 tcaaatcaaa atgattattt tgtaaatagc caatcgcctt gtcatagcga actgttgtaa
    20101 ccgcagctcc cgtttgcctt aaatagtttg ccaaagtatg aacaaacgaa tcttgatgat
    20161 cgataagtaa aactttttta tttttgccta ccagggggac atcctctgtc ttctttttag
    20221 ctttttgcca tggtttttgt aaaatgtcta aaaaagcaga agcttttaat ctcgtctctt
    20281 cttcctcagc ttcagggata gagtcataaa gtaacgttgc gcctactcta atttctgcag
    20341 ttcccttttc tattcgcact gttcttaaga ccaatccagt atttaaattt ccatcaaaac
    20401 caaaccatcc tacagcccct gcataccatt tacgcggcga tttctcatgt tgttcaatga
    20461 aattcatagc ccaaatttta ggcgcaccag ttacagtaac aacccacata tgagttaaaa
    20521 aagcatctac tgcatcaaat ccatctctta aaataccctc aacatgatca actgtatgaa
    20581 tcaatcggga atacatttcg atttggcgac gcccaatgac tttcacactc cctgcttcgc
    20641 atattcttga cttatcattt ctatccacat cggtacacat ggttagttct gattcttctt
    20701 tctctgaatc tagaagtgtt tgaatattgt gagcatcctc aatagcatct gctccccgtt
    20761 tgatagtgcc tgagataggg caagtttcta ctcttttacc ttggacacga acatacattt
    20821 caggtgaagc acctaccaga tattcttcgt cacccaaatt aacaaaaaat ccatatggcg
    20881 atggatttat ttctcgcatt tggcgaaata atattgacgg cttttcagca aagtaagtat
    20941 agaaagtttg gctaggaacc acttcaaata aatcaccaca ggaaaatttt tctttggctt
    21001 ttttgaccac ttcggcatat tcacctggct cgtgatcaca gtttttttcc ggtttatgga
    21061 taggttgata tggtgaatac tttccttctc tggataaaga ttgagtcgat cgcccctgat
    21121 attgaaaatc atagcgtcta acaaaggctt cttccttacg atgatttact atataaattt
    21181 catcaggtag atagaggacc atatctctct gtgtttcatt tcttggttta caccgctcca
    21241 actgttcaaa ttgataaatc aggtcaaacc caaaagcacc atataaaccc aagtatgaat
    21301 cttcttctgt tttaaaaaaa ttcaataaga ggcgaattgc actgaacact gacggttgat
    21361 ggctccgctc ctcctcacta aatactttgt ctgatgattt aatttttaac tgacaaaatg
    21421 aggtcgtttg actaattacc tcccagagat ctgttgtttt tagtaacgga tagagaaaag
    21481 ataataatat ctcgcctcgt tgatttaatg cctcaaaaca gatggtatta ctttggcaga
    21541 tcataactaa tgggggatta taaaaaccca tatcccaaca agtatagcgt cctggatatt
    21601 caaagctcga agcaaatact gctccacgtt gagaatcaag gctctcaagc aaaaaatcta
    21661 tgccttgttg ataattcaac tcttgttgag aaaactcaac atgaacacca ccgagagttt
    21721 tatattgttg taacatgaat accattctct accatttaat tagtacaaat attcattcta
    21781 ctgtaaaacc actgttctgc aagaatcttg taacatttca atatcattgc ttttttatgt
    21841 cttaagaaga tattgcaaaa cttgcttcaa aaaagacatg attgcagtca tgattaaaca
    21901 tggctctagg aaagcaactt ggctcaacag actaaaagat aattaaacta atacttcatt
    21961 tttagaacaa taccgctata atgtataacc cttatatttg gatatgacca tgactctctc
    22021 tgctaaagat cttgaaaata tttcaaaatt ggcttattta aatgaagact ctgagcattc
    22081 tgcaaaattg actcaagaag taagtgcgat catggatttt gttgaccagc tcaaaacaat
    22141 agatacaagc caaatagcac ctctttttca tcccttggca ctaaatcaac gcttaagacc
    22201 agatgaagta actgaagcgg aatgtcttgc tgaattagaa gccatggcgc cattatttga
    22261 ggataactta tatttggttc ccaaggtgat atcatcggat aaataataat ggaacattac
    22321 tcactggctc aattatcaaa ggctcttcat aatcgagaat tttcaagtgt tgaattaaca
    22381 caacactgca ttaacaaaat tcaaagcaat aaagacttga atgcattcat cagccttgat
    22441 gaagaccagg ctcttaaaga ggcgcaatcg gctgatttgg tactaaaaaa tggagaggga
    22501 aagccgctga ctgggatccc catggctctc aaggatttgt tttgtacaaa gcgattaaat
    22561 acaacttgtg cttcaaaaat gctggctaat tttcaagccc cctatgatgc cactatcgtc
    22621 acaaaattta aacaaaatgg cgcaataatt attggaaaaa ccaatatgga tgagtttgcc
    22681 atgggatcct ctaatgaaaa cagttacttc ggttcggtaa aaaacccgtg ggatagagaa
    22741 agagtgccag gtggatcatc aggcggttcg gcggcagcgg ttgctggaaa tctggtacct
    22801 tttgcaattg gttctgacac cggtggatca attcgtcagc cagcagcctt ttgcgggatt
    22861 agcgggataa aacccaccta tggattagtc tctcgatacg gaatggtcgc ctttgcctca
    22921 agccttgatc aagcgggacc atttgcgaag tcagctgaag atctggccat gatacttcac
    22981 tgtattgcgg ggtttgatag taaagattcc acgtcagttg atagagttat acctgattat
    23041 tcagcagaaa ttaaaaaacc agtagacaaa atacgcatag gacttccttc ctgctttttt
    23101 caacctcaag tcgaaaaagg tatccaggat gccatacata atgctgtgaa attatttgag
    23161 aatcttgggg ctgaaataat tgaaatcgac ttgaaactgc agccattttg ggtgccatgt
    23221 tactatgtta tagcttgcgc agaagcttca tcaaatcttt cgcgttatga tggcataaga
    23281 tttggccacc gaagcaaatc cgcatcaacc ttaattgaat taatcactaa ctctcgaagt
    23341 gaaggttttg gtaacgaagt aaaacgtcga attctaactg gaactcatgt gctctcatcc
    23401 ggtttttttg atgcctatta tttgcatgca caaaaagtga ggcgtttgat tcgagatgag
    23461 ttaattacaa ctttaaattc agtagatgtt atccttgggc caacaactcc taccaccgct
    23521 tttaaattgg gtgaaaaaat aaatgacccc atacaaaatt atttggcaga tgtatttact
    23581 gtagcagcca atcttgcagg cctaccagct atttctatac caactggttt tgaaaataaa
    23641 ctaccaattg gtttgcaatt aatgggtaag cactttagtg aaagccgctt gcttgcaatc
    23701 gcacatcatt atcagcaaca caccaactgg catttagcca atcctaacaa gcaagggtga
    23761 tatatggaat gggataccgt cattggtctt gaggtacatg ctcaattaaa aaccaaatca
    23821 aaattatttt caggagcatc tactgctttt ggtgccaccc ctaactcgca aacgagtttt
    23881 atcgatgctg gtttgcctgg tgttctccct gtattaaacg aacaagccat cataatggct
    23941 atccaatttg gtttggcaat tcatggaacc attaatgatc tgtctgtttt tgagcgtaaa
    24001 aattattttt atcctgactt gcccaagggc tatcaaatca gtcagtatca aaaacccatt
    24061 gttactaatg gttatttaaa tatacagctt ggtaataacc tggaaaaaac agtccatatt
    24121 gcacgcgctc atttagaaga agatgctggc aaatccctac acgatgccca tactgattac
    24181 accgggatcg acctcaacag agcaggtacc ccattattgg aaatagtcac aaccccttgt
    24241 ttgtactctg ctgaagaggc ggttaattat ttaaaaactc tacatcaatt agtgagattc
    24301 cttggaatat gcgatggcaa tatgcaagaa ggttcttttc ggtgcgacgt caatctgtct
    24361 ataaaaccta aaggcagctc agttttaggc acgcgaacag aacttaagaa tctgaattca
    24421 tttcgtttta ttgaaaaagc cattgctttt gaacaagcaa gacatcagga tatattggaa
    24481 agaggtctgt cagtcatcca ggagactcgc ctttacaatc ctgacaataa cacaactcag
    24541 gcgatgagag gcaaggaaaa tgaaaatgat taccgttatt ttcctgatcc tgatttatta
    24601 ccaattgata ttgacaaaga acaaattgaa gagattaaaa ataacctgcc ggacttgcct
    24661 gaagcaattt acaaagagtt aaaaaacact ccgtccctaa atgatgaaga cattaatttc
    24721 atattatcat caccggacac ttatcaatat tacaaaaaaa ttaaatccct ttgtccggct
    24781 gctgataaaa caattatcaa ctggttaaaa ggccaatatg ccgcctttct taatgaacac
    24841 aacctaacct ttgaaacacc accaatttca gctaaaacca tggctgcttt ccttagcaaa
    24901 attcatgaga aaaaaatctc ttccagtatt gctaaaaata ttttcagtat gctttgtgca
    24961 ggtgaagaag atatcgatgc gattattgaa cgcgaaggct accagcaaca gaatgacaac
    25021 tcggcactgg aagaaatagt tgagcaaata atcaaacaat atcccgaaca agttatagaa
    25081 tacaaagccg gtaaagagaa attattagct ttttttatcg gacaagcgat gaaacaaact
    25141 aaagggaaag caaatcccga gcaaatcaat ctgttattaa aaaaacatct tggatgatgc
    25201 aatattttta taattcacaa tgctcattta tggctttgtt gattgattgc cagctattat
    25261 catctttttg tcagaaggta taatgca
//
LOCUS       DAXLDT010000035        25108 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXLDT010000035 DAXLDT010000000
VERSION     DAXLDT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25108
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.035"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(19..3285)
                     /locus_tag="ACF2SP_001117"
     CDS             complement(19..3285)
                     /locus_tag="ACF2SP_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFD2358911.1"
                     /translation="MLNEAISKMPEAFEAKILLRGEEYFQKGQVLNIRLSDGLLKGRV
                     KGSASQIYDIHMDLKSWPAKPSRCSCAYQYNCKHAAACLFALRDREKINLQSLPSNKL
                     DRKLDVWLKNLRAQEASEIKREEATHHLTYLIDLKLRGYEHKVSIKLALAKLLKRGGY
                     GKKIIFNTLANSKKQHFMGDDNDIVAQLLFKCNVSGWFDNLLIRNTELLDKIIATGRA
                     FFIENEDTPIKKGETLTGECQWILSWNGNQILKLRHDNQNIEPLLLDESWYFDIKNVV
                     MGHLNSPYPIKQLRHLLDAPPIPLEQAELLAKKMSKNCPEFPMPNVFNQKETKQLKPI
                     PVLILDAVTEQEEHRWIDDGEENNSLYIAKVFFDYEGLLVPFAEECEKVVSLKGDVLV
                     EYQRDKEFEDLKIQELRNSIDLRPYHQWEKYHWNLPDHSFFILKDVQIVSDLESLYQQ
                     SLPYLKDHGWRIEFASPFYQEIVSADEVEWYSDIQESTTDFFSYQLGILVEGKPVSIV
                     PLVADLIQRYKGNDLDNLPDEQLVKLPLLDGRLLQLPMGRIKPLVRLLLQFGLRQIDQ
                     NLQLQINKYQLVLMQEVELAIAATKSRWQGAESLREELRKLVRLNDLPEIKIPSGLQA
                     HLRDYQHYGLNWLQFLRVSRFNGVLADDMGLGKTVQTLAHLQYEKEQSRLHKASLIIA
                     PTSLVGNWFAEAKRFTPEIRVLIYHGSDRHQDNFDDYDLIISTYGLIHRDKEKFVGYP
                     FYYLILDEAQFIKNARTKTTQIIQQLKASHRLCLTGTPLENNLGELWSLFHFLMPGLL
                     GDAKQFRLWFRTPIEKYADMGRRDLLAKRVQPFLLRRTKNQVANELPPKTEITRTIEL
                     VGAQRDLYEAIRMSMEKKVRDAIARQGLGKSHILLLDALLKLRQVCCDPRLLSMSEAE
                     IAHGTSCKLDALMELLDNLVEEGRRVLVFSQFTSMLKLIEELLIVRQYDYLKLTGQTQ
                     NRQALVDQFQQGDTPIFLISLKAGGTGLNLTRADTVIHYDPWWNPSVEDQATDRTHRI
                     GQESPVFVYKLITSGTVEEAILGMQEKKRQLVEGILSTDASRSMVLSEEDLNQFFMPL
                     "
     gene            3569..5179
                     /locus_tag="ACF2SP_001118"
     CDS             3569..5179
                     /locus_tag="ACF2SP_001118"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HFD2358912.1"
                     /translation="MNAPLTILMAQINPTVGAIDANTKKIIDVIQNHQANHDVIIFPE
                     LTLSGYPAEDLLFRKEYHDKIMLNLQDIQDTTKDCYVIVGHPMIHIGDCYNGFSIFYQ
                     GEKIRAYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCICEDLWQKGPVDDL
                     LDSGISILISLNASPFDYRKYQLREELLRSYAKRGVYIIYVNQIGGQDDLLFDGQSLA
                     MDNQGIIRALAPAFEESLCTVNIDGNKIDGSSTPYLDKEALIYKALVCGTRDYVRKNN
                     FPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASISNEDALEQLSNLKVA
                     HSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSN
                     KSETAVGYATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELK
                     PNQTDQDSLPEYGELDAIIMAYMEQNLSPSEIIAKGFKPQVVNKIIQLIKRNEYKRRQ
                     AAPGIKISNLAFGKDWRYPITNGFNQCI"
     gene            complement(5231..6679)
                     /gene="ravH"
                     /locus_tag="ACF2SP_001119"
     CDS             complement(5231..6679)
                     /gene="ravH"
                     /locus_tag="ACF2SP_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavH"
                     /protein_id="HFD2358913.1"
                     /translation="MDKEQGAYMPPKDGKELLDIVDNLNEFAEKLLSRIADESDEGCK
                     QLVHVLESARARYNKPIESWLKGWFYQFTRKRGQEIDMAIKGMEAFPDAYTRLQEFKL
                     LVGKGEWEHGSFNYYLFDELIKSVPGYKPLESELVHPIILKLRQKINKRIDEFMSQYL
                     ATQKLIEARKQELLQTHQSAQKFIDNVSIINDLEAAKKSAKENKGIIQFCLIFESDIW
                     KLSWVDATGKVYALEPGEELIQILIDGKIKDIVKDIDNLSALHLKQLKQECVKAREML
                     LGRVQLLINPKDPRTQAELTNDVLKENGTSATFVLRGKPNEYSLWWINTLGFAHEISL
                     EPYASMKTWLNDQSHPLKEEHIPQLKTYLLNVNTIKSIDVKEDMKEFKAQLQECLAAG
                     PKSKIPPASGAIKTTNAKKLDLRLFGDVERCLRSQLSERQEEKAAIQEPGKINLNKYE
                     NIATLFGNRAQNMEQHEGQHSKPNDSLSIRSI"
     gene            6845..7483
                     /locus_tag="ACF2SP_001120"
     CDS             6845..7483
                     /locus_tag="ACF2SP_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFD2358914.1"
                     /translation="MRITLLTTAILATGVAYAAVPVDGWYASAFGGYTYIPPNIYNFA
                     YGVFRDEVGYNNGYNAGGRVGYQSNPIRYEVEYTYLRGEARKFNLNFITQLGVTGFTS
                     ANTIMANIYYDCPDMLPAIAPFIGLGIGYASLRTELDSIGPFRPSYFTTSDSAFAYQG
                     TVGITYNFSENYAANLAYRYIATDNSSNFGKTYQAHLASVGAIYRFDQGNYK"
     gene            7493..8230
                     /locus_tag="ACF2SP_001121"
     CDS             7493..8230
                     /locus_tag="ACF2SP_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358915.1"
                     /translation="MKKLIQLSVLSGLLLSGSTFAFNQPQGFYVGILGEISHGPSGED
                     LIFTEPNRTITANVEYSPVSGGGGAVLGYKISHYRIEGEVLYNRISTGPFTISSCTLQ
                     SPNVLTPTGVCPSAFEVNRLGYDGSSAALYGLLNLYYDFFSSVNESTYVPYIGIGAGQ
                     VRIQNRNNFVKTTPVNLPASAGFSTTMVSGAGQGIVGVGYLMDDFAWLAMDFRFLTTS
                     SLSDFNNSRYTLSTFNVGINFTFDKST"
     gene            complement(8222..9274)
                     /locus_tag="ACF2SP_001122"
     CDS             complement(8222..9274)
                     /locus_tag="ACF2SP_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD2358916.1"
                     /translation="MNENHKELISIGLTVSIVIFSLYIVHKFIPSMIWAAIIVIATYP
                     LYTRWRKLFGNKHNTSAFLFTTLMGLLFLIPLSWLIGILIKESQLFINFLQHINKEGG
                     AAPEFFKNIPLVGDDLIQYWDVNIGKPGNIKEFLSNLHVTLTPTSYYIKQIGVNLAHR
                     SFQVGFTLLTLFFFYRDGDKLLLQIQHIGEYCLGDRWFRYSDRLPSALRGTVNGTIVV
                     GIGVGILMGVCYALVGFPAPTLTGFITALAAMIPFVVPVVFIIVALILLSVGSLIGGI
                     IVLVWGTLVMFVADHFVKPVLIGGAIQLPFLAVLFGILGGVETLGLLGLFLGPMVMVL
                     FVTLWQEPQGHKKSSG"
     gene            complement(9274..9756)
                     /locus_tag="ACF2SP_001123"
     CDS             complement(9274..9756)
                     /locus_tag="ACF2SP_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFD2358917.1"
                     /translation="MNQVNLANRVWQDPVYFIAFGFGSGLMSIAPGTWGTLAAIPLYL
                     LMINAHWSIYLLWTALAFALGVWVSDRVTEDLGVHDYKGIVWDEVVGYLLTMFLVPKG
                     ISWMLIGFILFRIFDIWKPQPIRMVDQRVHGGLGIMLDDVLAAVPAWCIMQILVWSFA
                     "
     gene            complement(9846..10802)
                     /gene="thiL"
                     /locus_tag="ACF2SP_001124"
     CDS             complement(9846..10802)
                     /gene="thiL"
                     /locus_tag="ACF2SP_001124"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFD2358918.1"
                     /translation="MNEFTLIDHYFKSIPCKRNDVLFGIGDDAACLQVPDGMHLLVST
                     DTLVSGIHFLPQWDPYDIACKAVMVNISDMAAMGAQPCWVTLALTLPELNQSWLDSFA
                     RGIKDSLGLYNVDLIGGDTTKGPLSITLTIMGLTPQGQAIRRCGAQAGDAIMVSGELG
                     AAGLAVKLLDEHKKVNAKDKVELMNKLLHPKPRVDLCHLLRKYATSAIDISDGLGADL
                     NHICAASGVGANLFEEAIPRHPLLNQYCPEQALDLALSGGDDYELCFTVSIQQLDSFN
                     RDLQKENLICFPIGVIEEKPGLRIQSADSPSKELQPRGYSHF"
     gene            complement(10795..11238)
                     /gene="nusB"
                     /locus_tag="ACF2SP_001125"
     CDS             complement(10795..11238)
                     /gene="nusB"
                     /locus_tag="ACF2SP_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFD2358919.1"
                     /translation="MEKQSIRGKRRARKFALQALYQWLMSGTDLHEIEAQFRTINNMD
                     KVDGEYFCRLLYGIPTHVEALEASLLPYLDREINALNPIELTVLRIGSFELFHCPEIP
                     YKVILDESVSLTKEFGSQEGYRYVNGVLNNLAKQVRSVEVSLDNE"
     gene            complement(11296..11763)
                     /gene="nrdR"
                     /locus_tag="ACF2SP_001126"
     CDS             complement(11296..11763)
                     /gene="nrdR"
                     /locus_tag="ACF2SP_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFD2358920.1"
                     /translation="MYCPFCHAEETKVVDSRLVADGAQVRRRRECLECHERFTTFETA
                     ELIMPLIIKRDGRREPFHIDNLRSGMLRALEKRPVSVDDLEKAIISITEEIRRRGERE
                     IDSQVVGELVMKELFRLDHVAYVRFASVYKRFKDVSDFRQTIDQMKNEDKEKS"
     gene            complement(11766..13019)
                     /gene="glyA"
                     /locus_tag="ACF2SP_001127"
     CDS             complement(11766..13019)
                     /gene="glyA"
                     /locus_tag="ACF2SP_001127"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFD2358921.1"
                     /translation="MFDESYSIKNFDDVLFKAISDEKRRQEEHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEELAISRAKLLFGAHYVNVQPHSGSQAN
                     AAVMMALLSPGDTFMGMALPHGGHLTHGSKVNFSGKLYHSVEYGVDSNTGLIDYDALE
                     KLALQHKPKLIIAGFSAYSRILDWARFREIADKVGAYLMADIAHVAGLVAVGLYPSPV
                     PYADVVTTTTHKTLRGPRGGLILCKENEEIEKKLNSSVFPGMQGGPLMHVIAAKAVAF
                     AEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEAD
                     AAVGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREITLLSNWVADVLDN
                     VHDETNISRVKTQVLLLCREFPVYA"
     gene            complement(13047..13358)
                     /locus_tag="ACF2SP_001128"
     CDS             complement(13047..13358)
                     /locus_tag="ACF2SP_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFD2358922.1"
                     /translation="MRDSLKMLLVGSISMIIIACAASPLSESTGEYLDSSTTTAKVKA
                     SLVDQLGTMGFAVKVKTYKDQVQLSGFVDSQKIKQRAGIIAAGVDGVKSVRNDLIIKT
                     R"
     gene            complement(13483..13920)
                     /locus_tag="ACF2SP_001129"
     CDS             complement(13483..13920)
                     /locus_tag="ACF2SP_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358923.1"
                     /translation="MKYLIPCVVLFPLLSSCTVIQEDFYQPEYLGPAPRVEVTPSYPQ
                     GHYHRHYNQRYHSNNRYYSAPRGQVHYEHAKTQGHAAVVTPPPSQARVEVQKNVHGHN
                     ANNGQVHGHAHDNGNVHSHPDSSNVHGHDTSNPEEQKKIHGHS"
     gene            complement(13986..14390)
                     /locus_tag="ACF2SP_001130"
     CDS             complement(13986..14390)
                     /locus_tag="ACF2SP_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358924.1"
                     /translation="MKLKSLLFVVCFSLFSNVFAANLHLHPKADNADKKSIQKSVTYP
                     GYCQIEIINQSFTDVRVFGTFDDGTTIDFNIYRYESPHYISLFYNFYCHSGMYITITS
                     PYYTIYSGWTNVNSTIRVVPYLKEAKAELSTR"
     gene            14631..15893
                     /locus_tag="ACF2SP_001131"
     CDS             14631..15893
                     /locus_tag="ACF2SP_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2358925.1"
                     /translation="MNKRLTIMGIILLVVFGGIIAFNLIKAYMIKQFFASYAPPAVTV
                     SSAVAQAVDWKPTIDAVGNFVAINGVDVNSEASGNVVQIHFESGQYVEKDEPLITIDD
                     SIDQALLKFNQSELTLKELSYKRQTDLFKRGATPSSSVDEAKANLQQAQAKVEQIQAQ
                     IKQKHIGAPFSGRLGIRQVNLGQYVTPGQTSIVSLQSLDPLYLEFYLPEQYYKRIHLN
                     QPVTFRVEEFPNILFEGVIHAVNSKVDLTTHNVQVQALLPNCPADAIQDPTKSPLIKA
                     RKQVRGDKLIVACSTELNKQNKIRNFVFIPGMFASIEVDQPAEPNTIIVPSTAISYSL
                     YGNAVYIIEKSKEGKKNKDGTDQLVVNRVFVTTGEQQGNYTVIKKGIKAGQLVVSTGD
                     LKLQNGTPVVINNSVKLDTDSDPNQLGQ"
     gene            16010..19057
                     /locus_tag="ACF2SP_001132"
     CDS             16010..19057
                     /locus_tag="ACF2SP_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2358926.1"
                     /translation="MKFTDLFIKRPVLSIVVSMLIFLFGINSIYKMQIRQYPKMENTV
                     ITIMTGYPGADAELIAGFITTPLESAVASAEGIDYMTSSSQQGLSTITLNIKLNFDPQ
                     IAFTDVMSKVQQTLNQLPKESQQPVILKKSDSSTALMYISLDSKDMTPQQITDYATRV
                     VQPQLQTVDGVAKADILGGQTYSMRIFMNPIKMAALNVTPADVSAVLASNNFLTAAGS
                     TKSEYVAINVTAKTDLNNVEEFERLIVKSDKGSIIRLRDIAKVELGSQNYDSSVTFNS
                     KKAVFISITPTPTANPLTVINDARKLFPSIVREFPPSLTGTIVYDATAFIRASIHEVM
                     ETIIEAAVIVIIVIFLFLGSVRSVLIPVVTIPLSLVGVCTLMLILGYSINLLTLLAFV
                     LAIGLVVDDAIVVVENVHRHLEEGKTPFESAIIGAREIATPVIAMTITLAAVYAPIGF
                     MSGLTGALFKEFAFTLASAVIISGVIALTLSPMMCSKILSEEATSGKFAIFLDEFFNK
                     LKRVYQNALHSLLDTREMMLLLAGVVILMLPYLYSNTAAETAPDEDQGFFFVMATAPQ
                     YATLDYIEAYTKPFDAIYKSFPETENYFTVNMSQPISGMVLKPWDQRKKSQFALKQPL
                     QNKLSKIAGLNAFAIVPPPLPGGGGGPAVQFVVKTTNDFQSLMDVSNKLTEKARNSGL
                     FIYIDNSLKFNQPQVEFSINRAKASEMGLDMRALGSSLTSALSGNYINYFNLEGRSYQ
                     VIPQLARKFRLTIEQLGQIYVKTLNGTMVPLSTVITPTEKTQPNAATHFQQLNSATIQ
                     AVMMPGKTLGQGLEFLQKAAQEVLPKGFTYDYGGESRQFMQEGSALIFAFLFAIVVIF
                     LVLAAQYESFRDPLIVLISVPMSICGALIPLNLGVATINIYTQVGLITLIGLISKHGI
                     LIVDFANHLQREKNLDKRAAVEESAGIRLRPILMTTAAMVFGVIPLLIASGAGAASRF
                     DIGLVISTGLLIGTCFTLFVVPTLYTYLAEDHRHKPNEPAEIQ"
     gene            19316..22081
                     /locus_tag="ACF2SP_001133"
     CDS             19316..22081
                     /locus_tag="ACF2SP_001133"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFD2358927.1"
                     /translation="MDKTYSPEAIEKALYKKWESHHYFQPRGEGKRFCIMLPPPNVTG
                     SLHMGHGFQHTIMDALTRYHRMLGDKTLWQPGTDHAGISTQLVVERQLEAQGVSRKDL
                     TREQFLDKVWQWKEESGNTITQQMRRLGASVDWSRERFTMDEGLSAAVQKVFVQLYEE
                     GLIYRGTRLVNWDPKLGTAVSDLEVLSEEEDGFLWHIRYPVVDSEEFLIVATTRPETL
                     LGDCAVAIHPDDSRFRHLIGKQVHLPLCDRTIPVIADDYVDKEFGSGCVKITPAHDFN
                     DHEVGKRHQLPQINILTKKGTINKNAPLKYQGMDRFVAREQIIKDLEKEGLLAKTEPH
                     KLKVPRGEKSNVIIEPLLTDQWYVKTKPLAEPAIAAVKKGDIRFIPETWDKTYFQWMD
                     NIEDWCISRQLWWGHRIPAWYDNHGNIYVGYSENDVRFKHKIDQSTPLKQDEDVLDTW
                     FSSALWPFSTLGWPERTPELEQFYPTSVLVTGFDIIFFWVARMIMMGLKFTGKIPFKE
                     VFITGLIRDSEGHKMSKSKGNVLDPLDIVDGIDLDSLIAKRTSNLMLNSVRDRITKAT
                     RKEFPEGISAYGTDALRFTYCSLASTGRNVRFDLGRVEGYRNFCNKLWNAARYVLLNT
                     DEEQIDFGDGAFQYSPADQWILSRLQNTVSKVHHYFETYRFDLLANTLYEFVWHEYCD
                     WYLELSKPILQDDQALSAMKRGTRRTLIHVLDQILKLLHPLMPFITEEIWQKTTKFTS
                     ENGISIMLSTYPKVNEEFINPAIEEELDWLKSAIQSLRTIRSEMSISPAKLIPLYIRN
                     ITPELKERIAKYEKILKTLSKIDKINYLAPDEKVPVSATAVLGEIELLIPMADLIDKE
                     AELSRLNKELAKLNKDIELAQGKLNNPKFTDKAPEEIIAKEKDKLAQAQVAKDKLLQH
                     KNRIESL"
     gene            22203..23450
                     /locus_tag="ACF2SP_001134"
     CDS             22203..23450
                     /locus_tag="ACF2SP_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFD2358928.1"
                     /translation="MVTQFNTLKSYIFPIILLLSILLGGLTGYVFNQNVAWLKPFGDI
                     FLNLIFTTIVPLIFFSVSSAIARTGSIGKLGKIFYSMTVIFLFTGIVAAIYALFVVLL
                     FPPAQNMPILLESSKQLSPTNLPERLIEIFTVPEFSKLFSHEHILALVLFSILVGLAV
                     AHSKDKGTIFNSFLKAGEDVFMSVFRLIMYYAPIGFFAYFAVIVSEIGPQLMENYLRI
                     AILYYTASVFYFFFALTAYAYLAGKSQGIRLFWGNIFLPAATSIATCSSAASIPANLM
                     ATKSMRIPAEIYETSIPLGSIIHKDGSVMGGVFKIAFLFGIFHLPFASLPVLMMALGV
                     SLLVGTVMGAIPSGGMLGELLILSVYGFPPSVLIAVAAISIIIDPIATMVNVTSNSVC
                     SMMIARLVEGKNWFLRSSDENNL"
     gene            23539..24000
                     /locus_tag="ACF2SP_001135"
     CDS             23539..24000
                     /locus_tag="ACF2SP_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358929.1"
                     /translation="MKKILYVIPGILLITNSFALDIPGNITNSNKDLLPSPFPVYVIE
                     GSAVVNHPYPGAKKVLLPTDNGYVDYPGCYIACYSHNTGVYAISPTISVMGQIRVKGQ
                     YDARICQPDGYKNQDISAAYQFKQLCAEKIPACQNNSCWAGGDTGGWFGIQ"
     gene            complement(24101..>25108)
                     /locus_tag="ACF2SP_001136"
     CDS             complement(24101..>25108)
                     /locus_tag="ACF2SP_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0716 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358930.1"
                     /translation="DDTNMSKNESGTAKPIIKPTLMFFNSKLTDLNDKRLQLNRGKGL
                     AKYLAKSQKNTRDLAPLDAALGVMSFSLYMLRFSANVGLLAHLILVESQEKTNHKIRR
                     DIYYSLLNDSLWCVVNLTQFFWLSYRNSISAGLQGMQLETLAQLIDILVMIIRYQQDK
                     KEYDLKYNQAMGMERSRLAIEWQNKELNGLRSLLTSLSVVIAFGLFSFSAVAVPLSPV
                     ISSIILISSLLRVLIDIEKDRQLIHQLKLNETSREQIKNEQLAMTNARMKDLNKIILN
                     SVFLPIGFFLLITTPIPLTIIACVSMLLVHWLITNLINMRYSPNISVSPTIQTAELSL
                     C"
BASE COUNT         7703 a         5277 c         4599 g         7529 t
ORIGIN      
        1 ataaaccgtt acttttattc ataaaggcat aaaaaattga ttcaaatctt cttcacttaa
       61 aaccattgac ctggaagcgt ctgtagacaa aatcccttcc actaattgcc ttttcttctc
      121 ttgcatacca agaattgcct cttctacagt acctgatgta atcaacttgt aaacaaaaac
      181 agggctttct tggcctattc gatgagttct gtcagtagcc tggtcctcaa cagaaggatt
      241 ccaccaggga tcataatgaa tcacagtatc tgcacgagtc agattaagtc ctgtgcctcc
      301 tgctttcaaa ctaattaaaa atattggcgt atcaccttgt tgaaattgat cgactaaggc
      361 ctgtctgttt tgggtctgtc ccgtcaattt caaataatcg tattgcctga ctattaataa
      421 ttcctcaatc agttttagca tcgatgtaaa ttgtgaaaat accagtactc gccttccctc
      481 ttcaaccaaa ttatcaagca actccatcag tgcatcgagc ttgcatgaag taccgtgggc
      541 aatctccgct tcagacatag acaacaatct tggatcacaa caaacctgcc gcaatttcaa
      601 caaggcatca agtaataata tatgactttt ccccaatcct tgccttgcta tggcatctct
      661 cacctttttt tccatgctca tgcggatagc ctcataaaga tcccgctgag caccaaccaa
      721 ctctatagta cgagttattt ccgttttggg cgggagttca ttggctacct gatttttggt
      781 tcggcgcagt aaaaaaggtt gcactctttt tgctaacaaa tcccttcttc ccatatcggc
      841 atatttttct ataggcgttc tgaaccaaag cctgaattgc tttgcatcac ccaatagccc
      901 gggcattaga aaatgaaata aagaccagag ttcgcctaaa ttattctcta gtggagttcc
      961 agtaagacat aaacggtgtg aagcctttaa ttgttgaatg atttgtgttg tttttgtccg
     1021 tgcattctta atgaattgcg cttcgtccaa aatgagataa taaaaggggt aaccaacaaa
     1081 tttttctttg tccctgtgaa ttaatccgta ggtagaaata attaaatcat aatcatcaaa
     1141 attgtcctga tgtctatcac ttccatggta aatcaatact ctgatctctg gcgtaaatcg
     1201 cttggcctcg gcaaaccaat taccaacaag actggtggga gctatgatta aactggcttt
     1261 atgtaatctt gattgttctt tctcatattg taaatgggcg agagtctgca ctgttttgcc
     1321 aagtcccata tcatccgcta atacgccatt aaaacgactg acccgcaaaa attgtaacca
     1381 atttaaccca tagtgttgat aatccctcaa atgagcttga aggccagacg gaatctttat
     1441 ttctggcaga tcatttaatc tgaccagctt ccttaattct tctcttaatg attcagcacc
     1501 ttgccaacgt gatttggttg cagcaatagc caattcaact tcctgcatta acaccagttg
     1561 atatttatta atctgtaatt gcagattctg atcgatttgg cgcaagccaa actgtaataa
     1621 caaacggacc aatggcttaa ttcgccccat gggcaattgc aataaacgac catccaacaa
     1681 tgggagttta accagctgct catcaggtaa attatctaaa tcattccctt tgtaacgttg
     1741 aatcaaatcc gcaaccagcg gcacaatact cactggcttg ccttcaacca aaatacccaa
     1801 ttgataggaa aaaaaatcgg tcgtactttc ctgaatatcg gaataccatt ccacttcatc
     1861 agcagaaaca atctcttgat aaaacgggct tgcaaactct atacgccatc catggtcttt
     1921 taaataaggt aacgattgct gataaagact ttccaagtct gaaacaattt gcacatcttt
     1981 taaaataaaa aaagagtggt cgggtaaatt ccaatggtat ttttcccatt gatgatatgg
     2041 cctcaaatca attgaatttc tcaactcttg aatttttaaa tcttcaaatt ccttatcacg
     2101 ttggtattca actaatacat ctccttttaa agacaccact ttttcacact cttccgcaaa
     2161 gggaacaagc aatccttcat aatcaaaaaa gacttttgct atataaagag aattattttc
     2221 ctctccgtca tcaatccacc tgtgttcttc ctgttcggta accgcatcga gaattaacac
     2281 aggaattggt tttaattgtt tcgtttcctt ttgattgaac acatttggca tgggaaattc
     2341 agggcaattt ttagacattt ttttagccaa taactcggct tgttcaagag gaataggtgg
     2401 tgcatccaac aaatgacgaa gctgcttgat aggataagga gagtttaagt gacccattac
     2461 cacattttta atatcaaaat accaactttc atccaataat aatggctcaa tattctgatt
     2521 atcatgcctt aattttaaaa tctgattgcc attccaggat aaaatccact ggcattcacc
     2581 agtcaaagtc tctccctttt ttataggggt atcctcattt tcaataaaaa aagctcttcc
     2641 ggttgcaatg atcttatcaa gaagctctgt attccgaatc aaaagattat caaaccaacc
     2701 ggatacatta catttaaata acaattgagc cacaatgtca ttatcatcac ccataaaatg
     2761 ttgttttttt gaattagcca gagtattaaa gattattttt ttcccgtaac cgccacgttt
     2821 taatagtttg gccaaagcta atttaatgct tactttgtgc tcataacccc gaagctttaa
     2881 atcaatcaaa taagtcagat ggtgtgtcgc ctcttctctt tttatttctg atgcttcttg
     2941 tgctcgtaaa ttttttaacc aaacatctaa ttttcgatct agtttgttag aaggcagtga
     3001 ctgcaggttt attttttctc tgtctcttaa tgcaaaaaga caagctgcgg cgtgtttgca
     3061 attatattga taagcacatg aacatcgaga aggctttgct ggccatgatt ttaaatccat
     3121 atggatatca taaatttgac ttgcactacc cttcactctc cctttgagca agccgtcact
     3181 taagcgaata ttcaatactt ggcctttttg aaagtactct tcaccccgaa gcaatatttt
     3241 agcttcaaaa gcctctggca tttttgatat tgcttcgttt aacatacctt ggatttgtcc
     3301 aggaaaaatc gtatattgta gtcatttttt acgctttttg acaatagaat gggttgttct
     3361 atccgctccc ttacggttgc ggctcggttt taaccatgat cctatctgac tcagagtgag
     3421 tcatgatccg agccgcgacc ataagtgagc ggataaatgc aaaaataaaa acttctctaa
     3481 ttaacctgac taccatcctc ttcatctgta gccatatcaa tgccctctaa gctacaattc
     3541 ctatatacaa attaattgac agattttcat gaatgcaccc ttgacaatac tgatggctca
     3601 aattaatcca acagttggag ctattgacgc gaatacaaaa aaaattattg atgtcatcca
     3661 aaaccaccag gcaaaccatg atgtgattat ttttccagaa ttaactctat ccggttatcc
     3721 ggcggaagat ttactctttc gaaaagaata tcatgataaa atcatgctca acctccagga
     3781 tattcaggat actaccaagg attgttatgt cattgtcgga catccaatga tacacattgg
     3841 ggattgctac aatggcttca gcatctttta tcaaggagaa aaaatcagag catatcacaa
     3901 gcaaaaattg ccaaattatg gagtttttga tgaagcaaga tattttactc ctggtaaaaa
     3961 agatccttgc gtcttatcaa ttaaaaacca taaattaggg ttttgcatat gtgaagattt
     4021 atggcaaaag ggtcctgtag atgatttact tgacagtggc atttccattt taatcagttt
     4081 aaacgcctcc ccctttgatt atcgtaaata ccagctgaga gaagagcttt taagatctta
     4141 tgctaaacgg ggggtgtaca ttatttatgt caatcaaatt ggtggtcagg acgatttgct
     4201 ttttgatggg caatctttag ccatggataa tcaaggtatt atccgtgctc tggctcctgc
     4261 ctttgaagaa agcttatgta ctgtcaatat agacggtaac aaaatagacg gatcaagcac
     4321 cccttacctc gacaaagaag cgcttattta taaggcactg gtttgtggta ccagagatta
     4381 cgtcagaaaa aacaatttcc ccggcgtact gcttgggctc tccggaggca ttgactctgc
     4441 cttaacttta gcaatagctg tcgatgcgct cggagcagag caagtccatg ctgtgttgat
     4501 gccctcacga tataccgcgt caatcagcaa tgaagatgca ctggaacaat taagcaacct
     4561 taaagttgca cattccatta taccgattga acccatgttt caatctatga ttgaatcgct
     4621 tcatcctgtt tttaaagact tgccaccaga taccacggaa gaaaatattc aggcaagaat
     4681 acgtgggatg ctgctcatgg ctttgtcaaa taaaacaggg aaaatggttc ttactaccag
     4741 taacaagagt gaaacagctg taggttatgc cactttatat ggcgacatgg ccggcggatt
     4801 tgccgtatta aaagatgtac tgaaaactca agtgtatgaa ttagtgaggt atagaaacaa
     4861 gatttcattg gttatcccag agcgagtcat taccagaccc ccttctgctg aattaaaacc
     4921 aaaccagaca gatcaggata gtttacccga atacggtgaa ctggatgcca ttatcatggc
     4981 ttacatggaa cagaatttaa gtccctctga aatcattgcc aaaggattta aacctcaagt
     5041 ggtcaacaaa atcattcaat tgattaaacg taatgaatac aagagacgtc aggctgcacc
     5101 aggaataaag ataagtaatc tggcctttgg taaagactgg agatatccta tcactaatgg
     5161 ttttaatcaa tgcatttgaa tgcaataact gccttgaaat aaaattcaag gcagttattg
     5221 catttattaa ttaaatagat cttatagaga gactatcatt tggttttgag tgttgacctt
     5281 cgtgttgctc catgttttgc gctctgttac cgaacaaggt ggcaatattc tcatatttat
     5341 ttagattaat tttaccaggt tcttgtattg cagccttttc ttcttgtctt tcacttaact
     5401 ggcttctcaa acaccgttca acgtctccaa ataagcgcaa atccagtttt ttggcatttg
     5461 tcgtcttgat tgcgccactg gcaggaggta ttttagattt tggtccggca gccaaacatt
     5521 cctgcaattg agctttaaat tccttcatgt cttccttgac atcaatggac ttgatagtat
     5581 ttacattcaa taaataagtc ttaagttgag gaatgtgttc ttcctttagc ggatgacttt
     5641 gatcatttaa ccatgttttc atagatgcat agggttctaa tgaaatttca tgtgcgaaac
     5701 ctaatgtatt tatccaccac aacgaatact cattaggttt accgcgcaaa acaaaagtag
     5761 cagaagtccc attttccttt aatacatcat tagtcaactc agcttgagtt ctcggatctt
     5821 tcggattgat gagcagttga actcgtccta gaagcatttc tcgggctttt acacactcct
     5881 gtttaagctg tttcaaatgc aaagcactta gattatcaat atccttaaca atatccttaa
     5941 tctttccgtc tattagaatt tggattaact cttccccggg ttccaaagca tacactttcc
     6001 ctgttgcatc tacccaggaa agcttccaaa tatcactttc aaaaattaag caaaattgta
     6061 taatgccctt attttctttg gctgattttt tagctgcttc caggtcattg ataattgaaa
     6121 cattatctat aaatttctga gcactttgat gtgtctgcaa caattcctgt tttcgagctt
     6181 ctattaattt ttgggtagcc aggtattgcg acataaactc gtcaattctt ttatttattt
     6241 tttgtctgag cttcaaaata attggatgga ctaactcact ttctaatggt ttatagccag
     6301 gaactgactt gataagctca tcaaataaat aataattaaa cgatccatgt tcccactccc
     6361 ctttcccaac caataactta aactcctgca agcgagtgta ggcatcaggg aacgcttcca
     6421 tgcctttaat tgccatatca atttcttgtc cgcgttttct ggtaaactgg taaaaccaac
     6481 ctttaagcca tgattcaatg ggcttattat atcttgcccg ggcagattcc aagacatgaa
     6541 ctaattgctt acatccttca tcggattcat ccgcaattcg acttaataac ttttcggcaa
     6601 attcgtttaa attgtcgact atatccaata actcttttcc atcctttggt ggcatatacg
     6661 ctccttgttc cttgtccaaa tttcatctta ccttaatcag aattttatac aagcaacaaa
     6721 atcattgagc taactggaat ttaaaattta aattgctcaa taaagcgcaa tagaaaactt
     6781 gcaaatttaa gacaaaccgc taacatgaat cagttaaaaa ataataataa taaaaaggga
     6841 tgtcatgaga attacattac ttacaacagc tatattagct acgggtgttg cctacgccgc
     6901 tgtacctgta gacgggtggt atgccagcgc atttggaggt tacacttata ttccccccaa
     6961 catatacaat ttcgcttatg gggtattccg tgatgaggtg ggatacaata acgggtataa
     7021 cgccggagga cgagtaggct accaaagcaa cccaatccgc tatgaagtag aatacaccta
     7081 tctcaggggt gaagccagaa agtttaatct taactttata acacagctcg gtgttaccgg
     7141 ttttacctcc gccaatacta tcatggccaa tatttactat gattgccctg atatgctgcc
     7201 agccattgcg ccatttattg gattggggat tggttatgcc tctcttagga cagagcttga
     7261 cagcataggt ccttttagac catcttactt taccaccagc gacagcgcct ttgcctacca
     7321 gggaactgtg ggaataactt ataatttctc cgaaaattat gcggcaaatt tggcttaccg
     7381 ctatatcgca accgataatt cgagtaattt tggaaaaacc taccaggctc atttggccag
     7441 cgtgggagca atctaccgtt ttgatcaagg aaattataag taaggatacg caatgaagaa
     7501 gttaattcaa ctctcggtat tatccggttt gctattaagc ggctcaactt ttgccttcaa
     7561 ccagcctcaa ggcttctacg ttgggatatt gggggaaata agtcatgggc cttctggtga
     7621 ggaccttata tttaccgaac ccaaccgaac cattactgcc aacgtggaat acagtcctgt
     7681 ttctggaggt ggaggggctg tattaggtta taaaataagc cactatcgca ttgaagggga
     7741 ggtactatat aaccggattt caactggtcc tttcacaata agttcctgta ctttacaaag
     7801 tcctaatgtc ctaactccaa caggcgtttg tccttccgct tttgaagtta atcgactggg
     7861 ttatgatggc agtagtgcag ctctctatgg tttgctcaat ctgtattatg atttttttag
     7921 ttctgtgaat gaaagtactt atgtacctta tattggaata ggcgcaggac aagtacgtat
     7981 tcaaaataga aataatttcg taaaaacaac tcccgtcaat ctgccggctt cagctggatt
     8041 tagcactacc atggtctcag gagctggaca gggcattgta ggcgtagggt atctaatgga
     8101 tgatttcgcc tggcttgcta tggacttccg ctttctcacg accagtagtc tgtccgactt
     8161 taacaacagt cgctatacac tcagcacatt caatgtgggt atcaatttta cctttgacaa
     8221 gtcaacctga tgacttcttg tggccttggg gttcctgcca caaggtcaca aacaaaacca
     8281 tcaccatcgg ccctagaaac aggccaagca aacccaaagt ttctactccc cccaaaatgc
     8341 cgaacaaaac agccaggaaa ggtaattgta tcgctccacc gataagaact ggctttacaa
     8401 aatgatcagc aacaaacata accaaagttc cccaaaccag aacaatgatg cctccaatca
     8461 aactgccaac ggaaagtaaa atcaaagcga ctatgataaa aacaacagga acaacaaaag
     8521 gtatcatagc tgccaatgcg gtaataaacc cagtcaatgt gggggctgga aacccaacca
     8581 aggcatagca aacgcccatt aatataccta cccctatgcc aacaacaata gttccattca
     8641 cagtacctcg caacgcgctg ggaagcctgt cggaataacg aaaccatctg tcacccaaac
     8701 aatattctcc tatatgctga atttgtaata gtaatttatc cccatcacga tagaagaaaa
     8761 aaagtgttaa aagggtaaat cctacttgaa aactacgatg agccaaattg accccaatct
     8821 gcttgatgta atagctggta ggggttaacg ttacatgcaa attagataaa aactccttga
     8881 tatttcccgg cttaccaata ttcacatccc aatattgaat taaatcatct ccaaccaatg
     8941 gaatattttt aaaaaactca ggggcggctc caccttcctt attaatatgc tgcaaaaaat
     9001 taataaataa ttgagactcc ttgataagga ttcctataag ccaactcaag ggaataagga
     9061 aaagcaagcc catcagtgtt gtaaataaga acgctgatgt gttgtgtttg ttaccaaata
     9121 atttacgcca tctggtgtat aagggatagg tagctataac aataatagcg gcccatatca
     9181 tggaaggaat gaatttatga acaatgtaaa gcgagaagat aacgatactg actgttaacc
     9241 ctatgctaat tagctcttta tgattttcat tcattaagca aagctccaaa ccaagatttg
     9301 cataatacac caggcaggaa ctgctgctaa aacatcgtcc agcataattc ctaacccacc
     9361 gtgaactctt tgatcgacca tacgtattgg ttggggtttc cagatatcaa aaatacggaa
     9421 caaaataaag ccaattaaca tccatgatat tcctttggga actaaaaaca tagttaataa
     9481 ataaccaacc acttcatccc atacaattcc tttgtaatca tgtaccccaa gatcttctgt
     9541 cactctgtca ctgacccaaa ctcccagcgc aaatgccagg gcagtccata ataaataaat
     9601 gctccaatga gcattgatca ttaataaata gaggggaata gcagctaatg tcccccacgt
     9661 gcctggagca attgacatca atccactgcc aaaaccaaaa gcgataaaat aaacgggatc
     9721 ttgccatact cgatttgcca aattgacttg gttcatattt gctcctcaac ctaaatatcg
     9781 ctatcacatc actttagaat cgaattttgt ctttaatttt tattatcata ctatagaggc
     9841 gggatttaaa aatgtgaata gcctctgggt tgcaattctt tacttggact atcagctgat
     9901 tgtattctta atcctggttt ctcctctatc acaccgatgg gaaaacagat cagattttct
     9961 ttttgaagat cccgattaaa agaatccagc tgttgtattg aaactgtaaa acataactcg
    10021 taatcatcac ctcctgaaag agccaagtca agggcttgtt caggacaata ttgattcaat
    10081 aaaggatgcc tgggtattgc ttcttcaaac aagttggccc caacaccact tgcggcgcaa
    10141 atatgattta aatcagcgcc taacccatcg gagatatcaa tcgctgaagt agcatatttt
    10201 ctaagcaaat ggcataaatc cacccgtggt ttaggatgta atagtttatt cattaattct
    10261 actttatctt ttgcattcac ttttttatgc tcatccagca attttacagc cagacccgca
    10321 gcccccaatt caccggaaac cataatcgcg tcacccgctt gtgcgccaca acgtctgatt
    10381 gcttgtccct gaggtgtgag ccccatgatg gtcaaagtga ttgataacgg gcctttggtc
    10441 gtgtcaccac cgatcagatc cacattatat aagccaagag agtcttttat ccctctcgcg
    10501 aaggaatcaa gccaactctg attcaattcg ggcaaggtca atgcaagagt cacccaacat
    10561 ggctgtgcgc ccatggcagc catgtcacta atattgacca ttactgcctt gcaggcaata
    10621 tcatagggat cccattgcgg tagaaaatga atgcctgaaa ctaaagtatc cgtgctgact
    10681 agcaaatgca ttccatcggg aacctgcaag caagcagcat catcccctat cccaaaaagg
    10741 acatcatttc tcttgcatgg gatggattta aaataatgat caatcagggt aaattcattc
    10801 attatcaaga ctgacttcaa cagaacgaac ctgttttgcc aaattattca ataccccatt
    10861 aacgtaacga tatccctctt gtgaaccaaa ctctttagtc aaggatactg attcatcaag
    10921 aatgacctta taaggtatct cagggcaatg aaacaattca aaagacccaa ttctcaatac
    10981 agtaagctct attggattca atgcattgat ttctctgtct agatacggca gtaaacttgc
    11041 ctccaatgct tccacatgag ttggaatgcc gtataacagg cggcagaaat attctccatc
    11101 tactttatcc atattattaa ttgttctgaa ttgagcttca atttcatgaa gatctgtacc
    11161 agacatcagc cattgataaa gggcttgcaa tgcaaatttt cgagctcgtc ttttaccacg
    11221 aattgattgt ttttccacaa ttacctcata ttattctaat aacaacaaga aaagtgagcc
    11281 cttcacattt tttatctaag acttttcctt atcttcattt ttcatttgat caattgtttg
    11341 tctaaaatca ctcacatctt taaagcgttt atacactgat gcaaaacgca cataggccac
    11401 atgatcaagc ctgaataatt ccttcattac caactcgcca accacctggg aatcgatctc
    11461 gcgttcacct cttcttcgaa tttcctcagt gatagaaata atagcttttt ccaaatcatc
    11521 aacgctgaca ggtcttttct ccaaggctct caacatgcca gaacgtaaat tatcaatatg
    11581 aaacggttct cttcttccat cacgttttat aatcaatggc ataatcaatt cagcggtttc
    11641 aaaagtagta aaccgctcat gacattccag acattcacgc cttctgcgaa cttgagcgcc
    11701 atccgcgacc agacgtgaat ccactacctt tgtttcttcc gcatgacaaa atggacaata
    11761 catgattagg cataaaccgg aaattcacga cagagtagca acacctgggt tttcactctt
    11821 gaaatattag tttcatcatg aacattatca aggacatccg ctacccagtt agataacaaa
    11881 gtaatctctc tttctttaaa gcctcttgtg gttgcagcag gcgtacccaa acgtaaacca
    11941 ctcgttacaa aaggcgagcg aggatcattg ggaaccgaat ttttattgac tgttatgtta
    12001 gctctgccta cagcagcgtc agcttcttta cctgtaattc ctttatttat caaatccact
    12061 agcaaaagat gattatcagt tccgccagaa acaatatcat agcctctgct ttgtaacaca
    12121 ctgcacatcg ttctggcatt agctaaaacc tgttgctgat aagttttgaa ttcgggtaac
    12181 agagcttctg caaaagcaac tgccttggct gcaattacat gcattaaggg cccaccttgc
    12241 ataccaggaa atacagaaga atttaatttt ttctcaattt cttcgttttc cttacaaagg
    12301 attaaaccac cgcgaggtcc tcttaaagtt ttatgagttg tcgttgtcac aacatccgca
    12361 taaggaacag gagaaggata taaccctaca gccaccagac cagctacatg agcgatgtcc
    12421 gccattaaat aagcgccgac cttatcggca atttctctaa atcgagccca atccaggatc
    12481 ctggaataag ctgaaaaacc ggcaatgatc aattttggtt tgtgttgaag cgcaagtttt
    12541 tccagagcat cataatcaat caaacccgta ttgctatcaa caccatactc gacagaatgg
    12601 tataatttac ctgaaaaatt aacctttgac ccatgagtca agtgcccacc atgaggtaaa
    12661 gccatgccca taaaagtatc gcctggggac aataatgcca tcatgactgc tgcgttcgct
    12721 tgtgatcccg aatgcggttg cacattgaca taatgagcgc caaaaagtaa ttttgccctt
    12781 gaaatggcta actcctcagc gacatcaaca aactcacaac ctccgtaata gcgtttgcct
    12841 ggatatcctt cagcgtattt attcgtcaac acagaaccct gagcctccaa tactctcggg
    12901 ctgacataat tttcagatgc tattaattca atgtgttctt cttggcgtcg tttttcatca
    12961 gaaattgctt taaaaagaac atcatcaaaa ttttttatac tataactttc gtcaaacatg
    13021 aatgttcctt ggttggaagg ttatatttac cttgttttta taataaggtc attacgaaca
    13081 ctctttacac catctacgcc agccgcaata atcccagctc tttgttttat tttttgagaa
    13141 tcgacaaaac cgctcaactg cacttgatct ttatatgttt ttacttttac ggcaaatccc
    13201 atagtcccca attggtcaac caaactggct ttcaccttgg cagtagtcgt tgaactatca
    13261 agatattccc ctgtactttc cgacagagga gaagcggcac acgcaataat tatcatggat
    13321 atggatccca ctaataacat cttaagtgag tcgcgcatag aggctcctta aagataaaaa
    13381 cgttattaga gtatcataaa aattacccgg atacgaagag gataatagta caattttagt
    13441 tcttttaaaa aaaaagtttc ctgtattcag caagttaaat tattaactat ggccatgaat
    13501 ttttttctgt tcctcaggat tggaagtatc atgtccatgt acattagaag aatcaggatg
    13561 gctgtgcaca ttaccattat catgagcatg tccatgcacc tgaccgttat ttgcattgtg
    13621 tccgtgaaca tttttctgaa cttcgactct ggcttgtgat ggtggcggag taactaccgc
    13681 agcatggcct tgagttttcg catgctcata atgaacctgc ccccttggcg cactatagta
    13741 tcgattatta ctatgatatc gctgattgta atgacgatga taatgccctt gaggatatga
    13801 gggggtaacc tctacgcgag gagcaggccc caaatactct ggctgataaa agtcttcttg
    13861 gataactgtg caggaagaaa gtaaaggaaa cagtactacg cagggaatga ggtatttcat
    13921 gtacaactcc tcaaaggaaa ggaaaactct cgaatcattg agagctttcc cgaattttca
    13981 acgaattatc tggttgataa ctcagcctta gcttctttca agtaaggaac tacacggatg
    14041 gtactgttca catttgtcca accggaataa atagtatagt aaggtgatgt aatagtaatg
    14101 tacataccac tatggcaata gaaattatag aataggctta tgtagtgagg tgactcataa
    14161 cgatagatat taaaatctat agttgtgcca tcatcaaacg taccgaatac acgaacatct
    14221 gtgaagcttt ggttaatgat ttcaatttgg cagtaaccag gataagttac gctcttttgt
    14281 attgatttct tgtctgcatt atcagccttg ggatgtaagt gaagatttgc agcaaaaacg
    14341 ttagaaaata aactgaaaca tacaacaaat aacaatgatt ttaacttcat gagcttcccc
    14401 ttagtaagat ggaagctgca tagtatcaaa gtattcaatt tagtcaatag gatttttttg
    14461 tacccataaa atcaaagttc attacaattc aattttatta gagaagaagc agaaacaact
    14521 atgaaaatat taaaaataat aagtcatctc aaacaataag atgataaagt aaatgtttat
    14581 gttatatttg ctaaattaaa atttttttca agcattaaca ggggaagata atgaataaac
    14641 gattgactat catgggaata atcttgttgg tagttttcgg tggaatcata gcttttaatc
    14701 taattaaggc ttacatgatt aaacaattct tcgccagtta tgcgcctcct gctgttaccg
    14761 tttcttctgc tgttgctcaa gcagtcgatt ggaagccaac cattgacgct gtaggtaatt
    14821 tcgttgcaat caacggagtt gatgtcaact cggaagcatc tggcaatgta gttcaaattc
    14881 attttgaatc agggcaatac gtagaaaaag atgagccatt gatcaccatt gacgacagca
    14941 tcgatcaagc attgctcaaa tttaaccagt cggaattaac gcttaaagaa ctcagctata
    15001 aacggcaaac tgatctcttc aagcgtggcg ccacgccaag ctccagtgtt gatgaagcca
    15061 aagctaactt gcaacaggct caggcgaaag tagagcagat tcaagcgcaa attaaacaaa
    15121 aacacatagg tgcccctttt tccggccgcc ttggcatccg ccaggtaaac ctcggccaat
    15181 acgtcactcc aggacaaacc tccatagttt ctttacaatc actggatccc ctttacctgg
    15241 aattttactt gccagagcaa tattacaaaa gaattcatct taaccagcct gtgacctttc
    15301 gcgtagagga atttcctaat attctctttg aaggagtcat tcatgcagta aactctaaag
    15361 tagatctaac tactcataat gttcaggtac aagctctatt accgaactgc cctgctgatg
    15421 caattcagga tccaaccaaa tctcctctta tcaaagcacg taaacaagtc agaggagata
    15481 aactgattgt ggcctgcagt acagaattaa acaaacaaaa taaaatacga aattttgtat
    15541 ttatcccagg catgtttgct tccattgaag tggatcaacc tgcagagcct aatactatta
    15601 tcgtgccttc cacagcaatt tcttacagtc tctatggaaa tgctgtttat atcattgaaa
    15661 aaagtaaaga aggtaagaag aataaggatg gtactgacca gttggttgta aaccgggtat
    15721 tcgtcactac aggggagcaa cagggaaatt acacagttat caagaaaggc attaaagcag
    15781 gccaattggt cgtaagtact ggtgacttga aattacaaaa tggtacccct gttgttatta
    15841 acaacagtgt caaactcgat acagacagtg atcccaacca actgggacag tagatcgtgt
    15901 tggcaacccg gtatttaaat cattttataa cattagcatg ctctaataat atggcttgtt
    15961 gttctttact accgactagg atcatggtca gttttttgca ggataactta tgaaattcac
    16021 agatctattc atcaagcgcc cggtactctc catagtggtc agtatgttga tttttctatt
    16081 cgggataaac tccatctata aaatgcaaat tcgccaatac cctaaaatgg agaatacagt
    16141 cataaccata atgaccggtt atcctggcgc tgatgctgaa ctcatagcag gatttatcac
    16201 tactcctctt gaaagtgctg ttgccagtgc tgaaggaata gattatatga cttcatccag
    16261 tcagcaaggc cttagcacaa ttaccttaaa catcaagttg aatttcgacc ctcaaattgc
    16321 ttttactgat gtcatgagta aagtgcagca aacactcaat cagttaccta aagagtcaca
    16381 acaaccggtt atcttgaaaa aatcagactc atcaaccgca ttgatgtaca ttagtctcga
    16441 cagtaaggat atgacccctc agcaaatcac tgactatgct acccgagtcg ttcaacccca
    16501 gttgcaaaca gtggatggag tagccaaggc tgatatttta ggtggtcaaa cttattccat
    16561 gcgaatattc atgaatccaa ttaaaatggc tgcgcttaac gtcactccgg ctgatgtgtc
    16621 agctgttttg gccagtaaca acttccttac tgcagccgga agcactaaaa gtgaatacgt
    16681 tgccatcaat gtcacggcta aaaccgattt gaataatgtt gaggaattcg aaagactgat
    16741 cgtaaaaagc gataaggggt caataatcag actcagggat atcgctaaag tggaactggg
    16801 ttcacaaaat tatgatagct cagtgacttt caacagtaaa aaagccgtct ttatatcgat
    16861 cactccaaca ccaactgcta accccctcac agtaattaat gatgctagaa aactgtttcc
    16921 ttcaatagta agggaattcc cgccatcatt aactggcaca atagtatacg atgccacagc
    16981 attcattcgg gcatctatcc atgaggtaat ggaaactatc attgaagccg cagtcatcgt
    17041 gattatcgtt atctttttat ttttaggctc tgttcgttca gttcttattc ccgttgtcac
    17101 tattccgctg tcattggttg gtgtctgtac tttgatgctc atactgggtt acagcatcaa
    17161 cttgcttacc cttcttgcct ttgtgttagc aataggattg gtagtggatg atgccattgt
    17221 agtggtagaa aatgtacatc gccatttgga agaaggaaaa accccctttg aatccgcaat
    17281 tataggcgcc agagaaattg ctaccccggt tattgcgatg acaatcaccc tggcagctgt
    17341 ttatgcacca atagggttta tgagtggact gactggcgct ttgttcaagg aatttgcttt
    17401 tacactggca agtgcggtta ttatttcagg ggttattgcc ttaacgctgt cccccatgat
    17461 gtgttcaaaa attctatccg aagaagccac tagtggaaaa tttgctattt ttctggatga
    17521 gttttttaac aaactgaaac gcgtttacca gaatgcactt catagcttat tggatacccg
    17581 agaaatgatg ttgctgttag caggggtagt gattttaatg ttgccctatt tgtattctaa
    17641 cacggctgct gaaactgcgc ctgatgaaga tcaaggcttc ttttttgtaa tggctactgc
    17701 tccacaatat gcaaccttag actacataga agcctataca aagccctttg atgccattta
    17761 taaaagtttt cctgaaacag aaaactattt tacggttaac atgagccaac ctatttcagg
    17821 aatggtattg aaaccatggg atcaaaggaa aaaaagtcag tttgctctaa aacaaccttt
    17881 acaaaacaag ttatccaaaa ttgctggact taatgctttc gctattgtcc cccctcccct
    17941 gccaggcggc ggtggcggtc ctgccgtaca atttgtggtt aagacaacga atgatttcca
    18001 gagcttaatg gatgtgtcga ataagttaac tgaaaaagcc aggaatagcg ggttattcat
    18061 ttacatagac aattccctta aatttaacca accacaagtg gagtttagca tcaatcgagc
    18121 aaaagcttcc gaaatgggct tggatatgag agcgcttgga agcagcctga caagtgctct
    18181 atccggcaac tacatcaatt acttcaatct ggaaggtcga agctaccagg taatcccaca
    18241 actggcgcgc aaattccgtc ttactataga acaattaggc caaatttatg ttaaaacgct
    18301 aaacgggact atggttccat tatccactgt gataacccca accgaaaaaa ctcaacctaa
    18361 tgcagctacc cattttcaac aactcaattc tgcaacgatt caggctgtga tgatgccagg
    18421 taaaacatta ggccaaggtt tggagttttt acaaaaagca gcccaggaag tcttgcccaa
    18481 gggatttact tatgattatg gaggagaatc caggcaattt atgcaagaag gaagtgctct
    18541 catctttgca ttcctttttg ctattgttgt tatcttttta gtgctggcag cgcagtatga
    18601 gagttttcgg gatccattaa tcgttctcat cagcgtaccc atgtccatct gtggcgcgct
    18661 tatcccgctt aaccttggag tagcgactat taatatctac actcaagtgg gtttgattac
    18721 tttaattggg ctcatcagta aacatggaat attgatagtg gatttcgcta atcatttgca
    18781 acgtgaaaaa aatcttgata aacgtgctgc tgttgaggaa tctgctggaa ttagattacg
    18841 acccatatta atgactacag ctgccatggt gtttggagta atacctcttc ttattgccag
    18901 cggtgctgga gcagcaagtc gattcgacat aggactcgtt atctccacag gtttattaat
    18961 aggtacctgt ttcacactgt tcgtagtacc tactttatat acctacttag ctgaagatca
    19021 tcgacataag ccaaacgaac ctgcggagat ccaataattc gactttggat tttttcgtaa
    19081 gccttattaa tctttaaaaa taattcgtca aacagcctgc atgtttcgtc ttctccgaag
    19141 catgcagccg tactttgtgg atacctgcga caagcaacgg tcattagaga atcggccatt
    19201 taaaacccaa aggactataa tgattttttt cctgagttcc tgttttttta aaatggctaa
    19261 attcccatac tctatgataa aattgtgagt ttttaccagt ataggctata cgataatgga
    19321 caaaacttac tctccggaag caatagaaaa agccctttat aaaaaatggg aaagtcatca
    19381 ttactttcaa ccacgcggtg agggtaaacg attttgtatt atgcttccgc cccctaatgt
    19441 aacaggaagc ctccatatgg gacatggttt tcaacacacc ataatggatg cattaaccag
    19501 atatcatcgc atgcttggtg acaaaacatt atggcaaccc ggtaccgatc acgccggcat
    19561 ctcaactcaa cttgttgtag aacgtcaact ggaagcccaa ggcgtatcga gaaaagatct
    19621 cacccgtgaa caatttctgg ataaagtgtg gcaatggaaa gaagagtccg gtaataccat
    19681 cacgcaacaa atgagacgtt taggcgcttc tgtagattgg agtcgagaac gtttcaccat
    19741 ggatgaagga ttgtctgctg ctgtacaaaa ggtatttgta cagctctatg aagaaggact
    19801 tatttaccga ggaacccgcc tggtgaattg ggatcccaaa ctgggcactg ctgtttcaga
    19861 tcttgaagtt ctctctgaag aagaagatgg ctttctttgg cacattcgct atcctgttgt
    19921 tgattctgaa gaatttctaa tagttgcaac tacacgcccc gaaaccttac taggtgattg
    19981 tgccgttgcc atccaccccg atgactcacg tttcaggcac cttattggaa agcaggttca
    20041 tctgccctta tgtgatagaa caatacccgt gattgcggac gattacgttg acaaggaatt
    20101 tggcagtggg tgtgtaaaaa ttacccccgc acatgatttt aacgatcatg aagtaggcaa
    20161 acgtcatcaa ttaccacaaa tcaatatttt aacaaagaaa gggacaatca ataaaaacgc
    20221 ccccttaaaa tatcagggga tggatagatt tgttgcccga gagcaaatca ttaaagattt
    20281 ggaaaaggaa ggcttgctgg cgaaaacaga gcctcacaaa ttaaaagtgc ctcgaggtga
    20341 aaaatcgaat gtcatcatcg aacctttatt aaccgatcaa tggtatgtta aaaccaaacc
    20401 attggcagaa cctgctatcg ctgctgtaaa gaaaggagac atccgtttta ttcccgagac
    20461 atgggataaa acctatttcc agtggatgga taatattgag gactggtgta tcagccgcca
    20521 actatggtgg gggcatagaa ttcctgcgtg gtatgataat catggtaata tttatgtagg
    20581 ttatagtgaa aacgatgttc gctttaaaca taaaattgat caatctactc ccttgaaaca
    20641 agatgaagat gttctggata cctggttttc atcagctctc tggccctttt caacattagg
    20701 ctggcctgag cgtactcccg aattggaaca attttaccct acttctgtac tggtaaccgg
    20761 atttgatatc atatttttct gggttgcccg tatgatcatg atgggactaa agttcactgg
    20821 gaaaattcct tttaaagaag tctttataac aggtttaatt cgtgacagtg aagggcataa
    20881 aatgtccaaa tccaaaggaa atgtactgga tccattggac attgtagacg gaattgatct
    20941 ggattcttta atagccaaac gaacgtctaa cctgatgtta aattctgttc gtgacagaat
    21001 tacaaaagcc actcgtaagg aatttcctga aggaatcagc gcttatggaa cagacgcgct
    21061 gcgattcact tactgctctc ttgcctcaac agggcgtaat gtgcgttttg atttaggacg
    21121 agttgaagga tatagaaatt tttgcaataa actatggaac gcagctcgct atgtcttatt
    21181 aaatacagac gaagagcaaa ttgattttgg ggatggcgca tttcaataca gcccagctga
    21241 tcaatggata ctatcccggc tacaaaatac cgtaagtaag gtgcatcact attttgaaac
    21301 ctatcggttt gatctcctcg ctaatacgct atatgaattt gtctggcatg aatattgtga
    21361 ctggtattta gaattatcca agcccatatt acaggatgac caggctttaa gcgccatgaa
    21421 aagaggcacg cgaagaactt taattcatgt tctggatcaa atattgaagc ttcttcatcc
    21481 tctgatgcca tttattacag aagagatttg gcaaaaaact accaaattta ccagtgaaaa
    21541 tgggattagt atcatgctga gcacatatcc caaagtcaat gaggaattca taaaccccgc
    21601 tatagaggaa gaattggatt ggttaaagtc agccattcaa tcactacgaa ccatacgcag
    21661 tgaaatgtct atctcacccg ccaagttaat acctttatac atacgcaata ttactcctga
    21721 attaaaggag cgtatcgcaa aatatgaaaa aattctcaag acgctaagca agatagacaa
    21781 aattaattat cttgctcctg atgagaaagt tcctgtttca gctactgcgg tattgggtga
    21841 aattgaattg ttgattccaa tggcagattt gattgataag gaggcagagt tatcaagact
    21901 gaataaagag cttgccaaat tgaataaaga catcgagctg gctcagggta aattaaataa
    21961 ccctaaattc actgataaag cgcctgaaga aatcattgct aaagaaaaag acaaattagc
    22021 tcaggctcaa gtggctaaag ataaactttt gcaacataaa aacaggattg agtcattata
    22081 actcaatgat ccttagttaa aacctcctta cagtcatggc tcaacttaac tcattattct
    22141 atacgtgact gtaaggaaca aatggcacaa actggattat ttaaaagaaa ggatgtcttc
    22201 tcgtggtaac tcaattcaat acgcttaaat cctatatttt ccccatcatt ttgctcttat
    22261 ctattttact tggtgggtta acaggatatg tttttaatca aaacgttgcc tggttaaaac
    22321 cctttggcga tatttttctt aatttgattt ttactaccat cgttccccta atttttttca
    22381 gtgtatcctc agcgattgca agaactggtt ctattggcaa attaggcaaa atattctact
    22441 caatgacagt catattctta tttacaggaa tagttgctgc aatttatgcc cttttcgtag
    22501 ttttactttt ccctccagcg caaaacatgc ccattctctt ggaaagttca aagcaactct
    22561 ctccgacaaa tctccctgaa cgcttgattg agatttttac tgtacctgaa ttttcaaaat
    22621 tattctccca tgagcatatt ctggcattag tccttttttc aatcctggta ggattggctg
    22681 tagcccattc aaaagataaa ggtacaattt ttaattcctt cttaaaagca ggagaagatg
    22741 tattcatgtc agtatttcgc ctgattatgt attatgctcc catcggtttt tttgcctatt
    22801 ttgcagtaat agtaagcgaa ataggccccc aattaatgga aaattattta cgcattgcca
    22861 tactctacta taccgcttca gtgttctact ttttcttcgc cttaactgcc tatgcttatt
    22921 tggcgggaaa atcccagggt atccggctat tctggggaaa tattttcctt cctgcagcga
    22981 cctccatagc aacttgtagt agtgccgcca gtattcctgc caacttaatg gcaaccaaat
    23041 ccatgcgcat ccctgctgaa atctatgaaa catcaatacc cttaggctcc ataattcata
    23101 aagatggatc agtcatgggc ggtgttttta aaattgcctt tttattcggg atctttcatt
    23161 taccttttgc cagcttgccc gttcttatga tggcattggg agtttctttg ttggtaggaa
    23221 cagtaatggg agcgattcct agtggcggga tgttaggaga attgttaatt ttgtcagtgt
    23281 atggttttcc accttctgtt ttaattgcag ttgctgctat tagcatcatc attgatccca
    23341 tagcgacaat ggtcaacgta acaagcaata gtgtttgcag catgatgatt gcacgtctgg
    23401 tcgaaggtaa aaattggttt ctcagatcct ccgatgaaaa taacctataa tctaaggtat
    23461 gtttaatata aaaagcacat attaaatttt cattatccca tatgtgcaat ataaaagcat
    23521 tttttggagg gaatagtaat gaaaaaaata ctgtatgtta ttccgggtat tttattaatt
    23581 acgaactctt ttgcccttga tataccaggg aatattacca attctaacaa agatctgctc
    23641 ccttcacctt ttcctgttta cgtcattgag gggtctgctg ttgtcaatca tccttacccg
    23701 ggagcaaaaa aagtactcct gccaaccgac aacggctatg tagattatcc aggatgctat
    23761 attgcttgtt attcgcataa tacaggtgtc tacgccattt ccccaacaat ttcagtcatg
    23821 ggacaaattc gagtcaaagg gcaatatgac gcccgaattt gccaacctga tggatataaa
    23881 aatcaagaca tcagtgctgc ttatcaattt aaacagcttt gtgctgaaaa gatccccgcc
    23941 tgccaaaata acagttgctg ggccggtggc gatactggcg gatggtttgg aattcaataa
    24001 gcagaccaat gaatttcaca gaataaaatg agataggata cttgattttg ggtcaaagac
    24061 acgcctttaa aatttttagc aagcatcatc actctcaact tcagcagaga gataattcag
    24121 cagtttgaat agtgggggat acggagatat ttggtgaata cctcatatta attaaattag
    24181 taattaacca gtgaaccaat aacatactta cacaggctat tatagttaaa gggattggag
    24241 ttgtgatcaa cagaaaaaaa ccaataggta aaaaaacgct attaagaatt attttattta
    24301 aatctttcat acgagcgttc gtcattgcca attgttcatt cttgatttgt tctcgactag
    24361 tctcattaag cttaagttga tgtattaatt gcctatcttt ctctatatcg attaaaactc
    24421 gtaaaagtga actaataaga atgatagaag atattacagg cgaaagtggt actgccactg
    24481 ctgaaaatga aaataatcca aaagctatca ctacagataa gcttgtgagc aaggatcgaa
    24541 gaccatttaa ttctttattc tgccattcaa ttgctaaacg agagcgctcc atacccatag
    24601 cctgattgta ttttaagtca tattcctttt tgtcttgttg gtaacgaata atcatcacta
    24661 aaatatcgat gagttgagcc aacgtttcta attgcatccc ttgaaggcct gcagatattg
    24721 agtttctata agataaccaa aaaaactggg tcaggttaac aacgcaccac aagctatcat
    24781 ttaaaagact gtagtaaata tcccttcgta ttttatgatt agttttttct tgcgattcga
    24841 ctaaaatgag atgcgctaat aaaccaacgt tagctgaaaa tcgaagcata tacagagaaa
    24901 agctcatcac acccaaagcc gcatctaagg gagcaagatc ccttgtgttt ttttgtgatt
    24961 ttgctaaata ctttgctaaa cctttaccac gatttaattg aagtcttttg tcatttaaat
    25021 cagtcagttt gctattaaaa aacattaatg tgggttttat gatcggtttt gctgtgcctg
    25081 actcgttttt gctcatatta gtatcatc
//
LOCUS       DAXLDT010000036        24997 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXLDT010000036 DAXLDT010000000
VERSION     DAXLDT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24997
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.036"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            313..3141
                     /locus_tag="ACF2SP_001137"
     CDS             313..3141
                     /locus_tag="ACF2SP_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HFD2358931.1"
                     /translation="MSEILEPVKDIPLTNQLELTANVPGMLKTIKRNGKVVIYDDTKI
                     KIAITKAFIADEGGTAPTSDRIHQQIEELTRQITQVFKRRLPSGGAIHIEEIQDQVEL
                     ALMRSGHYKVARSYVLYREERRKARESESKQKQTKDSKHLLITMPNGEVQPLDMDRVN
                     TIVKESCRDLSNVKAEPVIKDALRNLYNQAKYEDVHKALIMSARALVEKEPNYTYVSA
                     RLLLDSLRSEALTKLNIKSEATFDEMAQIYPVYFKSYIEQGIAQGMLDPKMADFDLDK
                     LSQALLPERDMKFTYLSLQTLYDRYFIHERGVRYELPQAFFMRVAMGLALREQHKEEK
                     AIEFYQLLSSFDYMSSTPTLFNSGTVRPQLSSCYLTTVPDHLDGIYSAIKDNALLSKY
                     AGGLGNDWTPVRAMGAHIKGTNGKSQGVVPFLNVADATAVAVNQGGKRKGAVCAYLEC
                     WHRDVEEFLELRKNTGDDRRRTHDMNTALWVPDLFMKRVREDGNWTLFSPDEVPDLHD
                     QYGKAFEALYVEYEEKARQGIIRNTKTISAVKLWRRMLSMLFETGHPWLTFKDPCNLR
                     SPQQHAGVIHSSNLCTEITLNTSEEEIAVCNLGSVNLPAHIKNGKLDEQKLKRTISTA
                     VRMLDNVIDINYYSVPQARNSNLQHRPIGLGIMGFQDALYELKMDYASKEAIEFADSS
                     MELISYYAIEASCDLAKERGSYSTYEGSLWSKGILPIDSINLLQQSRNKYLEQDRSQK
                     LDWEPLRVKVRTQGMRNSNVMAIAPTATISNICGVSQSIEPTYQNLYVKSNLSGEFTV
                     VNPYLVADLKTLNLWDEVMVNDLKYFNGSVQPISRIPEELKRRYATSFEIDPMWLVEA
                     ASRRQKWIDQAQSLNIYMAQPSGKKLDQLYMHAWIRGLKTTYYLRSLGASNAEKSTVT
                     DGALNAVKLIDTEPKACSITDPDCEACQ"
     gene            3155..4258
                     /locus_tag="ACF2SP_001138"
     CDS             3155..4258
                     /locus_tag="ACF2SP_001138"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HFD2358932.1"
                     /translation="MNFNDTQEIVVTPLMGATGLETLEMGAARIHVDDKQIINCRADL
                     NQLVPFKYKWAWDKYLTACANHWMPNEISMSADVALWKDPYGLTEDERLIIKRNLGFF
                     STADSLVANNLVLAVYRHITNPECRQYLLRQAFEEALHTHAYQYIIESLGLDEAEVFN
                     MYREIPSVSRKAVWALPFTQSLGDETFHTGTPENDQRLLRDLIAFYVVFEGIFFYVGF
                     TQILSMGRRNKMVGTSEQFQYILRDESMHMNFGIDVINQIKIENPHLWTPEFKNEIIQ
                     LIREGVELEYQYARDTMPHGILGMNAEMFEEYLHFIANRRLNQIGLPDQYPGAENPFP
                     WMSEMLDLKKEKNFFETRVIEYQAGGTLSWDDE"
     gene            4572..5054
                     /locus_tag="ACF2SP_001139"
     CDS             4572..5054
                     /locus_tag="ACF2SP_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1776 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358933.1"
                     /translation="MQSKIHKKEDNTNYSFLLKCMAAATAALATAAIVTAVVIKATAT
                     AAVGFGVATAGAALSPLLVVGGALLAAVGICCLPLLLCCGSSRSVYTVSSSPAYRPWS
                     SAWYTGYNPTFHSHPVYNNGGTYHTHHSGTVHTHSNPVHTHGNNVHVHATPSNVHGHR
                     "
     gene            complement(5141..6631)
                     /gene="lysS"
                     /locus_tag="ACF2SP_001140"
     CDS             complement(5141..6631)
                     /gene="lysS"
                     /locus_tag="ACF2SP_001140"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFD2358934.1"
                     /translation="MSEEHVHLDESEVYHIRKQKLAELRTSGFNFPNTFRREHLADAL
                     LKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDV
                     YEQFKHWDLGDIVGVQGELFKTNTGELTINAEYVELLTKSLRPLPDKFHGLADQELKY
                     RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVT
                     HHNTLDMTMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQA
                     YADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERLSVKEAILKYHPDIK
                     AQQLETLEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISP
                     LARRSDTNPEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHF
                     DSDYIEALEYGLPPTAGEGIGIDRLVMLFTNSQSIRDVILFPHLRQ"
     gene            complement(6628..7735)
                     /gene="prfB"
                     /locus_tag="ACF2SP_001141"
     CDS             complement(join(6628..7659,7661..7735))
                     /gene="prfB"
                     /locus_tag="ACF2SP_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031433237.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFD2358935.1"
                     /translation="MLEVNQINLSLIDLNERIESLRGYLDFEGKSERLEEVIRELESS
                     NVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTIIDIGDE
                     LKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHG
                     FTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFA
                     AVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQCQN
                     DRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEATKSDIGWGSQIRSYVLDQSRIK
                     DLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA"
     gene            complement(7795..8196)
                     /locus_tag="ACF2SP_001142"
     CDS             complement(7795..8196)
                     /locus_tag="ACF2SP_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HFD2358936.1"
                     /translation="MTHIFLYLHAIIFLLLGNYLLNQRKNINKLQNKIDSLEKLATQV
                     VKDHPMLINADLLFAKQLNEINQQLISIETQLQELENIRHNDGSYQHALRILEMGGTR
                     EEIISSCHLSNAEAELLMNLQAYRTALKHQN"
     gene            complement(8193..8996)
                     /gene="motD"
                     /locus_tag="ACF2SP_001143"
     CDS             complement(8193..8996)
                     /gene="motD"
                     /locus_tag="ACF2SP_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HFD2358937.1"
                     /translation="MKSRQKKTEEHQDSHRWVVSYADFITLLFAFFVVMYAISSVNVS
                     KYKSLSEGMKSAFNQKDQNKATQSTDNIKDGPEEKKTKGTFKDGLDELNKSLSELEDG
                     DFKLNRQEGWVEIDIKAGSLFDSGSADIKPEALIKLMKLAEKIKNLPFTVVVEGYTDN
                     VPIETPQYPSNWELSAARAATVGRILNGFGIANHRILVTGYGDQYPITDNYTEAGRSQ
                     NRRVNILITKNRKVDRILNPDLSRVHFSVISNTNSVELNKKDTKIKDKK"
     gene            complement(8971..9744)
                     /locus_tag="ACF2SP_001144"
     CDS             complement(8971..9744)
                     /locus_tag="ACF2SP_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HFD2358938.1"
                     /translation="MDTLTLIGLLTGFLAIIVGQIFEGGDLNSLLNFPALLIVMGGTL
                     GAVMVQTPLTTFKRAFRILPWVVKPPHHNFEECRTTLIELSRKARQLGLLSLEDRLDV
                     ETNPLIYKGLELLVTGVDKLTLRQVLEAEIDRTEHQDLRAAHVFESMGGYSPTIGILG
                     AVLGLIQVMRNLADPSQLGLGIAVAFVATIYGVGFANLFFLPVANKLKSCISHKVHFD
                     EMVVEGLVAMASGESPNMLQLKLNNYGQHKQDEIKTKEN"
     gene            complement(9754..10470)
                     /locus_tag="ACF2SP_001145"
     CDS             complement(9754..10470)
                     /locus_tag="ACF2SP_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFD2358939.1"
                     /translation="MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLD
                     DLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMI
                     SDAVRILEHKFGREAKDNEIAEELGVSLDDYHDMLQDSISSHLYGFDDLGVTDDVLFD
                     HEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSE
                     SRISQILSQATHRIRSRLPE"
     gene            complement(10567..11436)
                     /locus_tag="ACF2SP_001146"
     CDS             complement(10567..11436)
                     /locus_tag="ACF2SP_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HFD2358940.1"
                     /translation="MSKLSKGIKDQASGLRSLSRNKPVKVIAVSAGKGGVGKSNISVN
                     LAIALSQLNYSVMLLDADLGLANVDIMLGLHTKYNLSHVLQGICHLSDIILHGPAGVR
                     VIPAASGTEFMTQLSPAEHAGIIDSFNELTDDLDYMIIDTAAGISETVLSFTRSSQEL
                     IVVVCDEPTSLTDAYALIKVMSKRYEWTHFHILANMVRNVKDGRDLFNKLFRVSGQFL
                     DVQLDYLGAIPFDENIHKSVKKQNPILIAYPDSPAAQALRELAESVSNWSFKPSLGGN
                     TSFFLERLVAGQF"
     gene            complement(11423..12562)
                     /gene="flhF"
                     /locus_tag="ACF2SP_001147"
     CDS             complement(11423..12562)
                     /gene="flhF"
                     /locus_tag="ACF2SP_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HFD2358941.1"
                     /translation="MKLKRFVAADTRSAMQQIKAAFGPDAVILSSHNIKDGVEIVAAI
                     DFDETVISTQAAVAIAEPPSRTEKYSAQTPLDDMRQEILTLRGMLEAQLRGNVGIHEP
                     LHTVMLQKLLYLGVSPNTATIWARSVKPDLNQQRAWQFILSQMTEQLPIRDSSRIEEG
                     GIYAFVGPTGVGKTTTLAKIAARFALRFGADKLGLVTMDTYRIAAQEQLILYGKILGV
                     KVCIAQDEVSLARVLRQLNDKKLILIDTAGMNPSDERVTKQMTVLSNYLQSIEKVLVL
                     PATSHYQVLIDAIKRYQANHVDQCIITKLDESLAVGGAISAVIESNLALGYLTDGQRV
                     PEDIKIATRHQLVELFAQQELQLFQEARDISKINPNVRRENYVEA"
     gene            complement(12603..14681)
                     /gene="flhA"
                     /locus_tag="ACF2SP_001148"
     CDS             complement(12603..14681)
                     /gene="flhA"
                     /locus_tag="ACF2SP_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFD2358942.1"
                     /translation="MNNIMNYIEYWMRQGFGTPLILIVMISMMMLPLPPFLLDIFFTF
                     NIALSLVVLLAVIYSERPLDFAVFPTVILVATLLRLALNVASTRVVLLNGHNGTDAAG
                     QVIKSFGEVVIGGNYTVGIVVFIILVVINFVVVTKGAGRVSEVSARFTLDALPGKQMA
                     IDADLNAGLINQEQAIKRRTEVAQEADFYGSMDGASKFVRGDAIAGILVLIINIVGGL
                     IIGIFQYNMNFNDAVHNYILLTVGDGLVAQVPSLLLSTAAAIIVTRVSSAQNMGQAVI
                     SQVFAKPRSLVIAAAIIGLIGIIPGMPHIAFILLSAGLAGIAYLFINQEKVKREQQDK
                     EQNEFSQTSDSKLSELSWDDVNPVDVIGLEVGYRLIPMVDNAQGGILLSRIKGVRKKI
                     SQELGFLIPSVHVRDNLDLKPNHYRLSLAGVIMGESMIHPDKWLAINPGQIFGNLEGI
                     PTRDPAFGMDAVWINENQKEKAHTLGYTVVDASTVVATHLSMILEQNSQQLLGYEETQ
                     QLLDKLAISSPKLVKELVPDGLTLGMVSKVLQGLLSEHIPLTDIRTIVETLAEYASKS
                     KDTEYLISQVRIALSRLITQKISGLNEELPIITLKPELEQLLHNTIQNSGHGGVSFEP
                     GMADKIQQALIQFAAQQQAKQESSVLVVQPSIRTVLARFVRTISSDFHVLSYQEIPDN
                     KQIRIIGTVG"
     gene            complement(14678..15826)
                     /gene="flhB"
                     /locus_tag="ACF2SP_001149"
     CDS             complement(14678..15826)
                     /gene="flhB"
                     /locus_tag="ACF2SP_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFD2358943.1"
                     /translation="MADEEQSQEKTEQPSSKRLKESRKKGQVARSKDFNATVILLFTG
                     LGFFIFGKQLSLQIASIMQQAFDFDRDILVTGNNSLENLFQLTKSGLWSVVPLLLVIF
                     ILSLLAPLLMGGWVFSGQSIQPKFSRLNVLKGFKRMISLKGFVEMLKAFLKFVLVASA
                     SILVLRSQVPLLLELGKAPLEIAITSGVMILLKSFVLISASLILIAAIDVPFQLYEHS
                     KSIKMTKQELKDEYKETEGKPEVKSAIRRAQQETARRRMMSEVPKADVILTNPTHYAV
                     ALSYQKKGKKAPVVIAKGKNLVAFQISKVAKEHKIPIISVPALARAIYFSTKLNAEIP
                     RGLYVAVAQVLAYIFQLRDRQRYDYKPDILQNVPIPPELAREAEEEIE"
     gene            complement(15826..16596)
                     /gene="fliR"
                     /locus_tag="ACF2SP_001150"
     CDS             complement(15826..16596)
                     /gene="fliR"
                     /locus_tag="ACF2SP_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFD2358944.1"
                     /translation="MNLDYQTMIRLMSQFIWPMVRIGGLMLTVPVLSSALLPARIKIL
                     FVFTLSCVCFTYVPHNLSFENFNGLYLVYLVQELLLGLLMGFVLQIVFQVFILGGQII
                     SMQAGLGFAVMVDPTSNASVPLISQLYLMVTTLIFLALNGHLAILETLIESFRIMPIG
                     KSSLDPSIVWSTIMFSGWMFKEAVLISIPAILSLLIVSLSFGVMARVAPQLNLFSLGF
                     PITLLMGFVIIKIGLPTVGTEMVESIKHGIQFITGILR"
     gene            complement(16600..16869)
                     /gene="fliQ"
                     /locus_tag="ACF2SP_001151"
     CDS             complement(16600..16869)
                     /gene="fliQ"
                     /locus_tag="ACF2SP_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFD2358945.1"
                     /translation="MTPESVVSLFSDSVYIILLLLSVLILPGLIVGLIISMFQAATQI
                     NETSLSFIPKLFVTFLVLILAGPWLLKTLINYTDTLISNIPYLIG"
     gene            complement(16878..17627)
                     /gene="fliP"
                     /locus_tag="ACF2SP_001152"
     CDS             complement(16878..17627)
                     /gene="fliP"
                     /locus_tag="ACF2SP_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841869.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFD2358946.1"
                     /translation="MKIKSWLMILILLLLLPVAGHTDPLSFPVVTMQPGTNGSQSYSI
                     NLQILIFMTLLTVLPGLLMAMTSFTRIIIVLSILRQAIGMAQTPTNQILIGIALFMTY
                     FVMSPVFNKINETAIQPYMNERIDFSLALSNAQKPLRQFMLNQTRKNDLSLFEKFAHN
                     VPKTLDEIPMSVLIPAFITSELKTAFQIGFVLFLPFLIIDLVVSSLLMAMGMMMLSPL
                     IISLPFKIMLFVMVDGWTLVLGSLASSFALA"
     gene            complement(17628..18038)
                     /gene="fliO"
                     /locus_tag="ACF2SP_001153"
     CDS             complement(17628..18038)
                     /gene="fliO"
                     /locus_tag="ACF2SP_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFD2358947.1"
                     /translation="MKSKVLYLAFLSLSLISSVGWSSSSSPNAISHGELIRIISGLLF
                     VLFIIIFLSWIVKRMHRVNFTSSKGFQTVASMILGPKEKVVLLKIGDRYLLIGVGAYS
                     INLICDFGNQLPEGFDADQKSSFADVLKSAIGKS"
     gene            complement(18035..18370)
                     /gene="fliN"
                     /locus_tag="ACF2SP_001154"
     CDS             complement(18035..18370)
                     /gene="fliN"
                     /locus_tag="ACF2SP_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFD2358948.1"
                     /translation="MTQVNETSAEQLNQNQGNPLNPDSGNEKLELILDIPVSVTVEIG
                     RTKMTIRNLLQLNQGGIVALDRLAGDPLDVLVNGTLVAHGEVVVVNDKFGVRLTDIVS
                     KAERIKRLK"
     gene            complement(18380..19441)
                     /gene="fliM"
                     /locus_tag="ACF2SP_001155"
     CDS             complement(18380..19441)
                     /gene="fliM"
                     /locus_tag="ACF2SP_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFD2358949.1"
                     /translation="MTEKDVLSQEEIDALLDSVDESIDGETNDEELNSSDQPARKKPE
                     DTTSQELNSTFDSDIKVAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYH
                     LTARDFEIKQDPLLITKHKEFMKSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
                     YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVANLIHAWEPIIQLDITKFNDETNPQ
                     LVNIAEPEEMLLVARFVLNFGKETGSFYFILPYSMLEPIKQQLELGASRPDDEIDPNW
                     INSLKEELMDVELSVSASMAETVSTLGQVMSWKVGDFVPLEINEEVTLDIEGTPSFTA
                     TLGSTNEKRALKIIKIIRY"
     gene            complement(19886..20125)
                     /locus_tag="ACF2SP_001156"
     CDS             complement(19886..20125)
                     /locus_tag="ACF2SP_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358950.1"
                     /translation="MNKIFNLASIACFLFFSLIAFAADIPGLDEGTDKLNMEECVGEN
                     YQQCLDDVCLTSDEIDCNSNCQSLAEDKCEQQAVD"
     gene            20244..21539
                     /locus_tag="ACF2SP_001157"
     CDS             20244..21539
                     /locus_tag="ACF2SP_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFD2358951.1"
                     /translation="MRSKRILLTNFTRAIYSESCCIRPDTEKELIHYIAQNQPKNMLT
                     RGAGLSYSDSCLNQDGLVIDTSRFNHFIDFDVETGIVVCQGGTPFKDLFLLHDEFIPP
                     VLPGTAFATVAGGIAHDVHGKNNHSAGSFGHHISWFDLLIGDQIMHCSREKNSDLFFA
                     TIAGLGLTGIITQVAIRLKKAPRHLTIKNKPFPSIRELIAEMATYGLQQDYQVAWLDL
                     LYSEPRAILSTANYCESPNYPDEKLKIYSLPKLPFGLIKKWNMKVFNQYFYSRQKENE
                     MMSLIQYNNPLDKISHWNRLYGPRGLIQFQAVFDQDKAGQIINHILEIIRAHSATPTL
                     TVLKVFTQPGLGLLSFCRPGFTLAIDFINNPHAKKAIRAMNQFIAENDGCVYLAKDLL
                     LNPEQYFTMYKNQSKFAQILTKYRCPMRSDLATRLGIIK"
     gene            21536..22258
                     /locus_tag="ACF2SP_001158"
     CDS             21536..22258
                     /locus_tag="ACF2SP_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2358952.1"
                     /translation="MMQRKWVVLGATSVIAEQFAHLAAQAGNHLCLVARNSEQLALIS
                     KDIQLRYKTPCDAITINLSEPNEQLLTILETRERELDLFIAHCDFTDNNHLNPQSIKH
                     LIDTNISSTAILINTYLQSQQEKHRIVYLSSVASCRGRAKNSLYGASKAAIEIFLEGL
                     QQKATPHQHILIVRLGYIDTKQTYGLPGVFYAAQPHDCAKACWEKINHNKRLFYYPSF
                     WRVVMAIITKLPFFIYKRMGKI"
     gene            22377..23285
                     /locus_tag="ACF2SP_001159"
     CDS             22377..23285
                     /locus_tag="ACF2SP_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2358953.1"
                     /translation="MNIADLQSFLAVVELHSISLAAKKLHITQPALSKRIRKLESEWN
                     TQLFISSGLRTELTDKGKQLLPYVRQMVYLSHELISNTGTDKKKPQLLLNIGTTVYIA
                     QTIIPPLIIHMNALDLNYFINTKLIADKDVAHGLSEGAYDLIISPFMSNSAEIKSVPL
                     WREKLIPIVGLSHPLAKIKEKLSFAELAEFDAVLMEKNFIIRRIIDKEAEKQKLTLKI
                     RAEANIIYNNIALVEQGIAWSMINERLLNPNLKPLELSDYALSIDFYCHFLQKRTDER
                     LIRIFIDNLVNWVNASSELSSFSLVN"
     gene            complement(23364..24557)
                     /gene="mavB"
                     /locus_tag="ACF2SP_001160"
     CDS             complement(23364..24557)
                     /gene="mavB"
                     /locus_tag="ACF2SP_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavB"
                     /protein_id="HFD2358954.1"
                     /translation="MPPPYSTHVIVNVFRCVAQLVSQNPELLNWPGVFRLAGSREESE
                     KLLEQVINDNFSVGGLACYILEDGQINNLHLNNVLGMLPLVLKESVLLKTEDSLLKLF
                     MTELKNLLSSEKNNVENADAIVQLLSKFIDSLLLSKILDHQRVGEILYHYCYLMHTAG
                     VFHETNLMTWENLAIIMAPHFTNEFGLYPAEDLLGLIQFTNQLKPILECFIAHPGSGI
                     PFKERHADKLEHLANTRHTIVEKLTHMGSESRRIVVAPMKSLMLQASMLRAQIDAVET
                     QLKDSSLKKKDKKELNRQLEKLTEELEKLNIEISELTSKIKKMNHGHAKLKEEIRVIS
                     RSEDAVSTHATQQSGASSSSSNSVRIQLGIFGNNSSSSAYLIEEEEGEVTENEYSGLD
                     SAKLT"
     gene            complement(24794..24991)
                     /locus_tag="ACF2SP_001161"
     CDS             complement(24794..24991)
                     /locus_tag="ACF2SP_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HFD2358955.1"
                     /translation="MNDLLNLIAVIVVFGVGLWLINVFIPMPGAIKSLLNVLVLIILI
                     IYILQFFGVIKTILPMIKILK"
BASE COUNT         7910 a         5186 c         4259 g         7642 t
ORIGIN      
        1 acattatttc tcataaattt aacccccgca gagtatttta acccacaact tatccacaga
       61 ataatcccag attattctat tgaccaaagt aaaaaaacac attatcttgt tattaaaacc
      121 cctaaaacac cctatatctt gggttttttg ctataattat ggtaacagta aacaaaagct
      181 tctcttgacg gtcgtaaaaa caggagcaag tcttagtttc aacgaaacta ggattgcctt
      241 ttaaacaagt tcgtaaaaca tgttggattc actgaaacaa tgaattgcca aaatatattt
      301 cgtggaggaa aaatgtccga aattcttgag ccggtaaagg atataccgct aacaaatcaa
      361 ctggaattga ctgctaatgt cccaggcatg cttaaaacaa taaaacgaaa tggcaaggta
      421 gtcatttatg atgatactaa aatcaaaatt gctattacca aagcctttat tgccgacgaa
      481 ggtggtacag ccccaacttc tgatcgaatt caccagcaaa tcgaagagct aaccagacaa
      541 atcacccagg tatttaaacg ccgtttacct agtggtggcg ctattcatat tgaagagatc
      601 caggatcaag ttgaactcgc tcttatgcgc agcggacact ataaagtagc tcgctcctat
      661 gttttatata gagaagaacg tcgaaaagca cgagaatcag aatcgaaaca aaaacaaaca
      721 aaagacagta agcatttatt aataactatg cctaatggag aagtacaacc attagatatg
      781 gacagggtca atactatcgt caaagaatca tgtcgtgacc tcagcaacgt caaagcagaa
      841 cctgttatta aagatgcctt gcgtaatctt tataaccaag ccaaatatga agatgtccac
      901 aaagctttga ttatgtcagc gcgtgcgttg gttgaaaaag aacctaatta cacttatgtc
      961 agcgcgcgtt tgttgctaga cagcttacgt tctgaagcac tgactaaact caatatcaaa
     1021 tcagaagcta catttgatga aatggctcaa atctatccag tttatttcaa atcttacata
     1081 gagcaaggta ttgcccaagg tatgcttgac cctaagatgg ctgacttcga tctggacaaa
     1141 cttagccaag ctcttcttcc cgaacgagat atgaagttca cctatttaag tttacaaact
     1201 ttatatgatc gttattttat tcatgagcgc ggggtaagat atgagcttcc tcaagctttt
     1261 ttcatgcgag tagccatggg ccttgctctt cgtgaacaac ataaggaaga gaaagccata
     1321 gagttttatc agttactctc ttcatttgat tatatgtctt ccactccaac attatttaat
     1381 tccggaacag taaggccgca gttatccagt tgctatttaa caacagtacc cgatcatttg
     1441 gatggtattt atagtgctat aaaagacaat gcccttctct ctaaatatgc tggaggacta
     1501 ggcaatgact ggactccagt acgagctatg ggtgctcata tcaaagggac taatggtaaa
     1561 tctcaaggtg tagttccttt cctgaatgtt gccgatgcta ctgctgtagc agtaaatcag
     1621 ggggggaaac gcaagggagc tgtctgcgcg tacctggaat gctggcacag agatgtagag
     1681 gaatttctgg agttaagaaa aaataccggt gatgatcgcc gccgtactca cgacatgaat
     1741 actgcccttt gggtacctga tttgtttatg aagcgtgttc gtgaagatgg taattggact
     1801 ttattttcac ctgatgaagt acccgattta catgatcaat atggtaaagc atttgaggct
     1861 ctctatgttg aatatgaaga aaaagctcga caaggtatta ttaggaatac aaaaaccatt
     1921 tcagctgtca aactatggcg tagaatgctt tccatgcttt ttgaaactgg tcatccatgg
     1981 ctaacattca aagatccctg caatttacga tcacctcagc aacatgctgg agtaatacac
     2041 agttctaatt tatgtacaga gattactcta aatacttctg aagaagaaat tgctgtgtgc
     2101 aatttaggca gtgtcaatct tccagcgcat attaaaaacg gtaaactgga tgagcaaaaa
     2161 ctcaagcgca caatatcaac agctgtccgt atgttggata atgtcattga tattaattat
     2221 tattctgtac ctcaagctcg taactccaac ttgcaacatc ggccaattgg tctaggtatc
     2281 atgggctttc aggatgcgct atacgaatta aaaatggatt atgcatccaa agaagccatt
     2341 gaatttgctg actcttccat ggaactaatt agttactatg ctatcgaagc ttcttgtgac
     2401 ctggctaaag agcggggcag ttactcaacc tatgagggtt ctctatggag caaaggcata
     2461 ttgcctatcg attcaattaa cctcttgcaa caatcccgta ataaatatct ggagcaagac
     2521 aggtcacaaa aactggattg ggaaccatta cgagttaaag ttcgcacaca aggcatgcgt
     2581 aactcaaatg taatggctat tgcacctaca gccacaatat ccaacatctg cggtgtatca
     2641 caatctattg agccaactta tcagaacctt tacgtcaaat ccaacttgtc aggcgaattt
     2701 accgtggtga atccctattt ggtggccgat ttaaaaactc tgaacctttg ggatgaagtc
     2761 atggtcaatg atctaaaata ttttaatggc agtgtacaac ctatcagtcg aattccagag
     2821 gagctgaaaa gacgttatgc cacttctttt gaaattgacc ccatgtggtt ggttgaagcc
     2881 gcgtcacgcc gtcaaaaatg gatagatcaa gcccaatcct tgaacatcta tatggcccaa
     2941 ccttctggta aaaaactgga tcagctttat atgcatgcat ggattcgtgg attaaaaact
     3001 acttactact tacgtagttt aggtgcaagt aatgctgaaa aatccactgt taccgacggg
     3061 gcacttaacg cagtcaaatt aatagatacc gaaccaaagg cttgttctat tactgatcca
     3121 gactgcgagg cttgccaata atcaggagaa atttatgaac ttcaatgata ctcaagaaat
     3181 tgtcgtaact cctcttatgg gcgcgacagg gctcgaaact ttagaaatgg gggctgcacg
     3241 cattcatgtc gatgacaagc aaatcattaa ttgccgtgct gacttaaatc aattggtgcc
     3301 tttcaaatac aaatgggctt gggataaata cttgacagcc tgtgctaatc attggatgcc
     3361 caatgaaatc agtatgagtg ccgatgtcgc cttatggaaa gatccttatg gtcttacaga
     3421 agatgagcgt ttaatcatta agcgtaattt gggatttttt tcaaccgctg attcactcgt
     3481 tgcgaataat ctggttcttg ctgtttacag acatattact aaccctgagt gtcgtcaata
     3541 tcttttaagg caagcttttg aagaagcgtt acacactcat gcttatcaat acattataga
     3601 gagtttgggg ctggatgaag cggaagtatt taatatgtat agagaaatac cttctgtatc
     3661 cagaaaagcg gtttgggcat tgccatttac tcaaagctta ggtgatgaga ccttccatac
     3721 aggcactccg gaaaatgatc aacgcctgct acgcgattta attgcatttt atgtcgtgtt
     3781 tgagggtatt ttcttttatg tcggatttac tcaaattctg tccatgggac gtcgtaacaa
     3841 aatggtagga acatcagaac agttccaata tatactgcgc gatgagtcca tgcatatgaa
     3901 tttcggtatt gatgtgatta atcaaatcaa aatcgaaaat cctcatttat ggacacctga
     3961 atttaagaat gaaataattc aactcatcag agaaggcgtt gagctcgaat accaatatgc
     4021 cagagacact atgcctcatg gcattctcgg aatgaacgcc gagatgtttg aagaatattt
     4081 gcactttatt gcaaaccgtc gcttaaatca aataggtctg ccggatcaat accctggtgc
     4141 tgaaaaccca ttcccctgga tgagtgaaat gctggatctc aaaaaagaga aaaacttttt
     4201 tgaaactcgt gttattgaat atcaggctgg tggtacttta agctgggatg atgaataaaa
     4261 tttaatcatg ttaccttgag caaagtaagg gagttcattt agagaaatcc cttactttgt
     4321 gaaaaagtca tgccccagcc tttaacttaa ccaaacttaa taatattcta cgtatttcca
     4381 ttatttttga tttaattttg aacaaatcaa cctctaaatc caaatttatg gtgacatttt
     4441 agtatgatca ggtaaaatcc caagcgcata gaaataatgc tatgccagca aaataataac
     4501 aacatcgcta aaagatataa cacactcaga acaatgcatt ctattaactg aaaatcaaga
     4561 tgaggtaagt tatgcagtct aaaatacaca aaaaagaaga caatacaaat tacagctttc
     4621 tattaaaatg tatggctgct gctacagcag cacttgctac agcagcaata gtaaccgctg
     4681 tggtaatcaa agccactgcg acagcagccg taggttttgg tgttgcaacc gcaggagcag
     4741 ctctatctcc attattagtt gtaggaggag cattactagc tgctgtgggt atctgctgcc
     4801 ttccattatt gctctgctgt ggcagtagcc gtagtgtata taccgtatca agctcaccag
     4861 cttacagacc ttggagcagt gcatggtata caggctataa cccaactttt cattcgcatc
     4921 ctgtttataa caatggcggt acttaccaca ctcatcactc aggaaccgtt catacccatt
     4981 ctaatccagt acatacacat ggtaataatg ttcacgtcca tgctactcca agtaatgttc
     5041 atggacacag ataattttat ctaaaccaag tgtattttat gtattgcttg acatagcaat
     5101 ggtttgttta gaaccattgc tatctaaaaa tcgctgtact ttactgtctc aaatgcggaa
     5161 ataaaataac gtctctgatt gattgcgaat tagtaaataa catcaccaag cgatcgattc
     5221 caatcccttc accagctgta ggcggcaagc catattccaa tgcttcaata taatcactat
     5281 caaaatgcat tgcctctaaa tcacctgcat ccttttcttc aacttgcttt cgaaagcgct
     5341 cagcttgatc ctcggcatcg tttaattcag agaatccatt cgcaatctca cgaccagcaa
     5401 taaaaaactc aaatcgatcg gtgacttccg gattggtatc agaacgtctc gctaaaggag
     5461 atatttctgt agggtattcc gtaataaaag tgggctgaaa taattggtgc tctactgtct
     5521 cctcaaaaag aataatttgt agtttaccca ggccatcagt ttctttataa ggcagaccta
     5581 aatcattcag aagtgttctg catccctcta acgtttctaa ctgctgcgcc ttaatatcag
     5641 gatgatattt taatatagct tcttttacgc tcaaccgttc aaaaggttta ttgaaatcaa
     5701 tgacttgacc ttgatattct atttgccgag tagctaatac tttatcgcat agataatgaa
     5761 acaattgttc agtgaaattc atcaaatcat tgtaatcggc ataagcctgg taaaattcca
     5821 gcatcgtaaa ctctggatta tgacgagtag aaattccttc gttacggaaa tttctattaa
     5881 tttcatatac tctctcaaaa ccaccaacaa ccaagcgttt taagtacaac tcgggggcta
     5941 tcctcaaata catggtcata tccaatgtat tgtgatgagt tacgaaagga cgagccaaag
     6001 cacctccagg aattggatgc atcattggtg tttctacttc cagaaaatga ttatcatcca
     6061 taaattctct gaaggctttg attaaatgtg aacgtattaa aaaagttttt cgactgtctt
     6121 catttgcaat caagtcaaca taacgtttac gatatttcaa ttcctgatca gccaaaccat
     6181 ggaatttatc aggaagcggc cttaaagatt tggttaataa ctcaacatat tctgcattaa
     6241 tggtcagttc accagtgttt gttttaaata actcaccctg tacaccaaca atatctccta
     6301 aatcccaatg tttaaactgt tcataaacgt caggaagatc atttgaacga agataaactt
     6361 gtatccgacc tgatacatcc tgaatatgaa aaaaactggc tttacccata attcttctca
     6421 gaacgattcg gcctgcaaca gatactttca catgcttttg ttctaatgtt tgtttttcag
     6481 tttcggagta ttgcttcagc aaagcatcgg ctaaatgttc gcggcgaaag gtatttggaa
     6541 aattaaaccc gcttgtgcgc aattccgcta atttttgttt gcgaatatga taaacttcac
     6601 tttcatctaa atgtacatgt tcttcactca tgcttgccct actcctgcct ttaaactggc
     6661 ttcaataaat tgatctaatg aaccatccaa tactgcctga gtattactgg tttcaacacc
     6721 ggtgcgcaaa tcttttatac gagattggtc taatacgtaa gaacgaattt gtgatcccca
     6781 accaatgtca gatttggtag cttccaatgc ttgctgctct gcatttttct tttgcatttc
     6841 caactcataa agcttcgccc tcaattgctt cattgcctgg tctttgtttt tatgctggct
     6901 tcgatcattc tggcattgaa cgactatccc actagggata tgtgtaatcc ttacggcaga
     6961 atcagtacgg ttaacatgct gcccgccagc cccggaagct ctgtaagtat caatccgcaa
     7021 atcagctgga ttaatttcaa tttcgatatc atcgtcaatt tcaggagaga caaaaacagc
     7081 tgcaaacgaa gtgtgtctcc tgttgccaga atcaaatggc gactttctca ctaaacgatg
     7141 gacacctgtt tcagtcctta accaaccaaa cgcatattca cctgtaaaat gaatagtcgc
     7201 acttttaatt cctgccactt caccaggaga gcattctatc aactcggtgg taaatccgtg
     7261 atgttctccc caacgtaaat acattcgtaa taacatttca gcccaatctt gcgcttcagt
     7321 gccgccagaa cctgcttgaa tatcaagata agcattggag tggtccattt tgccagaaaa
     7381 cattcttcta aattccaaac tggcgacttg ttgctcaatt gattttaatt catcaccaat
     7441 gtcgatgatg gtttgctcat catcttcttc tcgagccaat tcaaacagtt ctctctgatc
     7501 actaattgat tggcttaatt ggcttaatga gtggactata gtttccagct gagttctttc
     7561 acgtcccaat gcctgagcct gttccggatt gtcccacaca ttggatgatt ccaattcgcg
     7621 aattacttct tccaagcgct cacttttacc ttcgaagtca aagatacccc ctaagcgatt
     7681 ctatgcgctc attaagatcg attaaactta aattaatttg attcacttct agcatcagtg
     7741 tctcttttaa aaataaatag gagattttac agcgaaagta agattctatc tatactaatt
     7801 ctgatgtttt aatgcggttc tatacgcctg aagattcatt agtaactctg cttcagcatt
     7861 tgataaatga caactactga taatttcctc acgagtgcca cccatttcca ggattctcaa
     7921 ggcatgttga taactgccat cgttatgtct tatgttttcc aattcttgca attgagtctc
     7981 aatactaatt aattgctgat taatttcatt aagttgtttc gcaaataata aatccgcatt
     8041 gatcagcata ggatgatctt ttactacttg agtagccaat ttttccaaag aatctatttt
     8101 gttctgtaat ttattgatgt tttttcgttg atttaataaa taattaccca agagtaaaaa
     8161 aattatcgca tgcaaataca gaaagatatg tgtcattttt tatccttaat tttagtatcc
     8221 tttttattta actctactga atttgtatta cttataacac taaaatggac tctacttaaa
     8281 tctggattga gaattctgtc cacttttcta tttttagtga taagaatatt cactcttcga
     8341 ttctggcttc ttccagcttc agtgtaattg tccgttatgg ggtattgatc accatatcct
     8401 gtaaccaaaa tacgatgatt ggcaattcca aaaccattta aaatgcgacc aacagtagct
     8461 gctcttgcag ctgataattc ccaatttgaa gggtattggg gcgtttcaat aggcacatta
     8521 tcagtgtatc cctcaacaac cactgtaaaa ggtagattct taattttctc cgccagcttc
     8581 attagtttaa tcaatgcttc tggtttaata tcagcactac cagaatcaaa taatgagcct
     8641 gcttttatat ctatctcaac ccatccctct tgtctgttta atttgaaatc accatcttca
     8701 agttcagata gtgatttatt cagttcatcc aaaccatctt taaaagttcc cttggtcttc
     8761 ttttcttcag gaccatcttt aatgttatct gtcgattgag tcgctttatt ttgatccttt
     8821 tgattaaatg ccgacttcat tccttcagaa agagatttat attttgaaac atttactgaa
     8881 gaaatagcat acatcacaac aaaaaaagca aataataagg taatgaaatc agcataagat
     8941 acgacccatc gatgactatc ctgatgttct tcagttttct tttgtcttga tttcatcttg
     9001 cttgtgttgc ccatagttgt ttaactttaa ctgcagcata ttgggactct caccactcgc
     9061 catggcgacc aggccttcaa cgaccatttc atcaaaatgc accttatgag aaatacaact
     9121 ttttaattta ttagcaacag gtaaaaaaaa cagattcgcg aacccaacac cataaatagt
     9181 tgcaacaaaa gcaaccgcaa tgcctaaacc taattgactg ggatcagcga gattcctcat
     9241 gacttgaatt aaacccaaca ccgcacctaa aattcctatg gtggggctat agccgcccat
     9301 gctttcaaag acatgcgcag cacgcaaatc ctgatgctcc gttcgatcta tttctgcttc
     9361 caaaacttgt cgtaaggtaa gcttgtccac tcctgtaacc agtaattcca accccttata
     9421 aattaaagga ttggtttcga catccaaacg atcttctagg gagagaaggc caagttggcg
     9481 agcttttcta gataactcta tgagagtcgt tctgcattct tcaaaattat gatgaggagg
     9541 tttcactacc caaggcaata ttctaaacgc ccttttaaaa gtggttaaag gagtttgcac
     9601 cataacagcc cctaatgttc cacccatgac aatcagtaag gccggaaaat taagtaagga
     9661 atttaaatct cccccctcaa aaatttggcc tacgataatg gcgagaaaac ctgtcaacaa
     9721 tcctattaat gtcaaagtat ccatacttat tttttattcc ggtaatcttg atctaatacg
     9781 gtgcgttgct tgacttaaaa tctgtgaaat tcgtgactca ctaacctcca gtatttcccc
     9841 gatttccttc aaattcaaat cctgctcata atacaaagaa agcaccagcc tctctttacg
     9901 agggagacta tcaatcactt gggttaatcg attcatcata tcacttcgca taacattcct
     9961 gtgcggttca gttgagccat tcccttcatg atcaaataag acatcatcgg ttactcccaa
    10021 gtcatcaaaa ccatataaat gactgctaat ggagtcttgc aacatatcat gataatcatc
    10081 taatgacaca cctagctctt cagcaatttc attatcctta gcctcacgcc caaacttatg
    10141 ctccaatatg cgcacagcat cagaaatcat tctggaatta cgataaacag aacgcggcac
    10201 ccaatcgtta cgtcttactt catcaagcat atgtcccctg atacgtattc ctgcataagt
    10261 ttcaaaggat gcccccttag atgaatcata atgccttgct gcttcaagca gaccgagcat
    10321 ccctgattgt attaagtcat ccagttgtac actctgcggt aatcttccga gcagatgatg
    10381 tgcaatgcgt tttaccaatg tggcatgtgt ttttaccaga gcttcctggg tttgttgatt
    10441 tactttgctg tatgcagcca aagcatccac gatactctcc ttaccataac agcagattgt
    10501 agttattagt aatttggtaa atctaaaaca aacagagtac agctaatttt aagtatagcg
    10561 aataatttaa aattgtcccg caaccaaacg ctccaaaaag aaacttgtgt ttccacctaa
    10621 agacggttta aatgaccaat tactcaccga ttcagctaat tctctcaagg cttgagcagc
    10681 aggtgagtcg ggataagcga ttaaaatagg attttgtttt tttactgatt tatggatgtt
    10741 ttcgtcaaaa ggaatcgcgc caaggtaatc taactgcaca tccagaaact gtcctgagac
    10801 ccgaaacagt ttattaaaca agtcacgacc atccttaaca tttctaacca tattagccag
    10861 aatgtggaaa tgcgtccact cataacgttt gctcatcact ttaattagtg cataagcatc
    10921 tgtcaaagag gtaggttcat cacagacaac aacaatcaat tcttgcgatg aacgagtaaa
    10981 actcaatacg gtctcggaaa tccctgctgc ggtatcgata atcatatagt ccaaatcatc
    11041 agttagttcg ttaaatgaat caatgattcc cgcatgttcc gcaggactca attgcgtcat
    11101 aaactctgtt ccagaagcag caggaataac tctgacgcca gcgggaccat gtaaaataat
    11161 atcacttaaa tgacatatac cctgaagaac atgagataaa ttgtatttgg tatgcaaccc
    11221 cagcataata tccacatttg ccaatcctaa atcggcatcc agaagcataa cactataatt
    11281 tagttgagat aacgctatgg ctaaattgac cgagatatta cttttgccta caccaccttt
    11341 ccctgccgaa acagcaatca ctttaacagg tttgtttcgg gacaaactcc taagaccaga
    11401 agcctgatct tttatacctt tgctaagctt cgacataatt ctctctccta acattcggat
    11461 ttattttgct gatgtccctt gcctcctgaa ataactgaag ttcttgttgc gcaaataact
    11521 caacaagctg gtgacgcgta gcaattttga tatcctcagg gactcgttgt ccatcggtta
    11581 aataacccaa agctaaattt gattcaataa cagcacttat ggctccacct actgcaagag
    11641 attcatccaa tttcgtaata atgcattgat ccacatgatt tgcctggtaa cgcttaatcg
    11701 catcgattaa tacctggtaa tgactggtag ctggtaatac caatactttt tctatagatt
    11761 gcagataatt acttaaaaca gtcatttgtt tggttacacg ttcatcagaa ggattcatcc
    11821 cggctgtatc aatcaaaatt aattttttgt catttaattg acgcaatact cttgccaagg
    11881 agacttcatc ctgcgcgata catactttaa cccccaaaat cttcccatag agaattaatt
    11941 gttcctgagc tgctattcgg taggtatcca tagtgaccaa acccaacttg tcagcgccaa
    12001 aacgtaaagc aaagcgtgct gcaatttttg ctagagttgt tgtcttacca actccagttg
    12061 gaccaacaaa cgcgtagata ccaccttctt ctattctact agaatcacga attggcaatt
    12121 gctctgtcat ttgtgacaaa atgaattgcc aggctctttg ttgattcaaa tcaggcttta
    12181 ctgagcgagc ccaaattgtc gccgtattgg gactaacacc taaatacaag agtttttgca
    12241 gcataactgt atgtaaaggc tcatgaatac caacatttcc acgcagctgt gcttcgagca
    12301 ttcctctcag tgtcaaaatt tcttgccgca tgtcgtctaa tggcgtttgt gctgaatatt
    12361 tttctgttcg gcttgggggt tctgctatgg ctacagcagc ttgagttgat atcactgttt
    12421 cgtcaaaatc aatagcagca actatttcaa caccatcttt tatgttatga cttgacaaaa
    12481 taacagcatc agggccaaaa gcagccttta tttgctgcat ggcactgcga gtatctgcag
    12541 cgacaaagcg ttttaatttc ataatgcctc ctgccttcaa cacacctatg caaccccaaa
    12601 aactaaccta cagttccaat tattcttatt tgtttgttat caggtatttc ctgataagac
    12661 aaaacatgaa agtcactaga tatagtacgc acaaatcgtg ccaaaaccgt tcgtatactt
    12721 ggttgaacta ccaaaactga gctctcctgc tttgcctgtt gttgtgcagc gaactggatc
    12781 agtgcttgtt ggattttatc tgccattcct ggttcaaagc taactccgcc atgtccactg
    12841 ttttgtatag tattgtgcaa taattgttcc aactctggtt ttaatgtaat aatgggcaat
    12901 tcctcgttta atccactgat tttctgagtt atcaatcgtg ataaggcaat tctcacttgg
    12961 ctaatcaaat attcagtatc ttttgatttt gacgcatatt ccgccaaagt ttcgactata
    13021 gtgcgtatgt ctgttaatgg aatatgttct gataataatc cctgcaacac tttgctaacc
    13081 atacctaaag taagtccatc aggtacaagc tctttaacca gtttagggga acttatagcc
    13141 aacttatcca atagctgctg cgtttcctca tagccaagta attgctggct gttttgttcc
    13201 aggatcatac ttaaatgagt agcaactact gttgatgcat caactactgt ataacctaaa
    13261 gtgtgtgctt tctccttttg attttcattt atccacacag catccatccc aaaagccgga
    13321 tctcttgttg ggattccctc taaattacca aatatctgtc cggggtttat tgctaaccat
    13381 ttatcaggat gaatcattga ttcacccata atcaccccag ccaagcttaa tctgtaatga
    13441 ttaggcttta aatccaggtt gtcacgaaca tgaacggatg gaattaagaa acccaattct
    13501 tgcgatattt tctttcggac acctttaatt cgcgaaagta atattccacc ctgagcatta
    13561 tcgaccatag ggatcaatcg ataacctact tccaaaccaa tgacatcgac aggattaaca
    13621 tcatcccatg ataactcact cagtttagag tcactggttt gtgaaaattc attttgctct
    13681 ttatcctgct gctcacgttt aactttttcc tgattgataa ataaataagc aataccggct
    13741 aaccctgctg ataatagaat aaatgcaata tgaggcatac caggaattat acctattaga
    13801 ccaataatag ctgctgcaat gacaagtgat cttggttttg caaatacttg agaaataact
    13861 gcctgcccca tattttgggc gctagaaact cgggtcacaa tgattgccgc tgcagtagat
    13921 aataagaggg agggaacttg agcgaccaaa ccatccccga ctgtcaataa aatataatta
    13981 tgaactgcgt cattaaaatt catattgtat tgaaatatgc ctattattaa tccaccaacg
    14041 atgttaatga ttaaaaccaa aattcctgct atggcatccc ctcggacgaa tttactggca
    14101 ccatccattg aaccgtaaaa atccgcttcc tgagctactt ctgttcgacg cttgattgcc
    14161 tgttcctgat tgattagacc tgcattgaga tctgcatcga tagccatttg ctttccgggt
    14221 aaagcatcca aagtaaaccg cgcacttact tcagatactc tccccgcccc ttttgtcact
    14281 acaacaaagt taataacgac aaggataata aataccacta tacctactgt gtaattacct
    14341 ccaatcacca cttcaccaaa agattttatg acctgaccgg cagcatctgt accattatga
    14401 ccattgagta agactactcg tgtagaagct acattgaggg ctaatcttaa caaagttgcc
    14461 acaagtatca ctgtgggaaa caccgcaaaa tccaggggtc tttcactata aataactgcc
    14521 aacaaaacaa ctaaagaaag agcgatatta aaagtaaaaa atatatccag taggaaagga
    14581 ggcagaggta acatcatcat gctgatcatg acaatcagta ttaatggagt accaaaccct
    14641 tggcgcatcc aatattctat ataattcata atattgttca ttcaatctcc tcctctgctt
    14701 cgcgagccag ttcaggtgga atagggacat tttgtaaaat gtcaggctta taatcataac
    14761 gttgcctgtc acgtagctga aaaatatagg ccagcacctg agccacagca acatataacc
    14821 ctctgggaat ttctgcattc agttttgttg aaaaataaat agcgcgagct aacgcaggaa
    14881 ctgaaataat agggatctta tgctctttag caaccttgga tatctgaaaa gcaaccaggt
    14941 ttttaccctt ggcaatcacc actggagcct tttttccttt cttttggtaa ctaagagcaa
    15001 ctgcatagtg cgtaggatta gtcaatatga cgtcggcttt aggtacttca ctcatcatcc
    15061 gtcgtcttgc agtctcctgc tgtgcgcgtc gtatagcgct ttttacctca ggtttgcctt
    15121 ccgtctcttt gtattcatct tttaattcct gtttagtcat cttaatcgat ttactatgtt
    15181 catataattg gaatggaaca tctattgccg caattaatat cagactcgca gaaatcagca
    15241 caaaagattt taaaaggatc attaccccac tagtgattgc tatttctaat ggtgctttcc
    15301 ccaattcaag caacaagggt acttgcgatc taagcactag aatagatgcg ctagccacca
    15361 ggacaaattt taaaaaagcc ttaagcatct caacaaatcc tttgagagaa atcatgcgct
    15421 taaacccttt aagtacattt aatcgagaaa attttggctg tatagactgt ccactaaaaa
    15481 cccatccccc cataagcaat ggcgctagca aagataaaat aaaaataacg agcaatagcg
    15541 gaacaacaga ccaaagccct gattttgtta attgaaataa attttctaaa gaattattcc
    15601 cggtaacaag aatgtcacga tcgaaatcaa acgcctgttg cattatactg gcaatttgca
    15661 gtgataattg tttcccaaaa ataaagaaac caagtccggt aaataataaa atgaccgttg
    15721 cattaaaatc tttggaacgt gcaacctgcc ccttttttcg tgactctttt aatcgcttgg
    15781 aagaaggctg ttccgttttt tcttgtgatt gttcttcatc ggccatcagc gcaatattcc
    15841 tgttataaat tggattccat gcttaatgct ctctaccatt tctgtcccga cagtgggcaa
    15901 acctatttta atgatgacaa accccatcaa tagcgtaatc ggaaacccta aagagaaaag
    15961 gtttaattgc ggagcaaccc gagccataac tccaaaagac aagcttacaa taagcaaaga
    16021 taaaatcgca ggaattgaaa tcaataccgc ttctttgaac atccaacctg aaaacataat
    16081 cgtactccag acaattgatg gatccagaga agatttaccg attggcatta ttctgaaact
    16141 ctcgatcaga gtttcaagaa tagctaaatg gccattcaat gccaaaaata ttaatgtggt
    16201 caccattaaa tacaattgac tgattaaagg cacactggca ttgctggttg gatcaaccat
    16261 tactgcaaat cccaaacccg cctgcatgga tataatttgc cccccaagaa tgaatacttg
    16321 aaatactatt tgcaacacaa atcccattaa caaacccaat aataattcct gtacgagata
    16381 gacaagataa aggccattaa aattttcgaa agacaagtta tgaggcacat aggtaaaaca
    16441 cacacagctt aaagtaaaaa caaagagaat tttaatgcgt gccggcaata gtgctgaaga
    16501 taaaaccggc accgtaagca tcaaaccacc aatacgcacc atgggccaaa taaattggct
    16561 cattaagcga atcatagttt gataatcgag attcattttt tatccaatca aataaggaat
    16621 attgcttatc aaagtatctg tataatttat caatgtcttt aataaccaag gccctgctaa
    16681 aattagaacc aaaaaggtaa caaataactt gggtataaaa cttaaacttg tctcattaat
    16741 ttgagtcgca gcctgaaaca tcgatataat tagtccaaca attaatcccg gtaatatcaa
    16801 cactgacagt aatagcagaa tgatataaac actatcacta aacaatgaaa cgactgattc
    16861 aggggtcata tagcctccta ggcaagagca aaacttgaag caagtgaacc caaaaccaaa
    16921 gtccatccat caaccatcac aaataacatg attttaaagg gtaatgaaat aatcaagggt
    16981 gaaagcatca tcatccccat agccatcaaa aggctggaca caacaagatc aataattaaa
    17041 aagggtaaaa aaagaacaaa gcctatttga aatgcggttt ttaactcgct agtaatgaaa
    17101 gccggaatga gaacactcat aggaatctca tctaaagttt ttggtacatt gtgagcaaat
    17161 ttttcaaata aagataaatc attttttctc gtttgattta gcataaactg acgaagtggc
    17221 ttttgcgcat tggataatgc cagagaaaaa tcaatccttt cattcatata gggctgtatt
    17281 gctgtttcat taattttatt gaatacaggt gacataacaa aataagtcat aaataaagca
    17341 atgcctatta atatttgatt ggtaggcgtc tgagccatcc ctattgcctg ccgtaaaatt
    17401 gacagaacaa taattatcct tgtaaatgac gtcattgcca tcagcaaccc tggcaatacg
    17461 gtcaataaag tcataaaaat taaaatttgc agattgatgc tataagattg cgaaccatta
    17521 gttccaggtt gcattgtaac aacgggaaag gacaaagggt ccgtgtgacc tgcaacgggt
    17581 aataaaagaa gtaatatcaa aatcattaac caacttttaa ttttcattta tgatttccct
    17641 attgctgatt tcagcacatc ggcaaaagac gatttttgat cagcatcaaa cccttcaggc
    17701 agttgattgc caaaatcaca tatgagatta atggaatatg cccctacacc tattaataaa
    17761 tatctatccc caattttcag caatacaacc ttttcctttg gacctaaaat catacttgca
    17821 acagtttgga atcctttaga tgaagtaaaa tttactctat gcatacgctt cactatccaa
    17881 gataaaaaaa taataatgaa taatacaaat aagagtcccg aaatgattct aatcagttcg
    17941 ccatggctga tcgcgtttgg gcttgaagaa gatgaccaac cgaccgaact gattagagaa
    18001 agacttaaaa atgccagata gagtactttg cttttcattt gagacgtttt attcgttcag
    18061 ccttgctaac gatatcagtt aatctgacac caaatttatc gttaactaca acgacttcac
    18121 catgtgcgac taaagtacca ttgactagca catcaagggg atcaccagcc agcctatcaa
    18181 gagctacgat tcctccctga tttaattgca gtaaattacg aatagtcatt ttggtacgac
    18241 cgatttcgac agttactgag acaggaatat ctaaaattaa ctccagtttc tcattgccag
    18301 agtctggatt cagtggattc ccttgatttt gatttaattg ctctgcgctg gtttcgttca
    18361 cttgagtcat ccttatcccc taataacgta ttattttaat aattttcaaa gctcgctttt
    18421 cattcgtact gcctaaggtt gcagtaaagc ttggtgtccc ttctatgtcc aaggttactt
    18481 cttcatttat ttccaaaggc acaaaatctc ctactttcca tgacattacc tgacccagag
    18541 tactgactgt ttccgccatt gaagcactaa ctgacaattc aacatccatg agctcttctt
    18601 tcagagaatt aatccagtta gggtcaattt catcatcagg tcttgatgcc cccagctcca
    18661 attgctgctt gataggttca agcatggagt atggtaaaat aaagtaaaaa gaccctgttt
    18721 ccttaccaaa attcaagacg aaccgcgcca caagcaacat ttcttctggc tcagcaatat
    18781 taactaattg gggatttgtt tcgtcattga attttgttat atccagttga attattggtt
    18841 cccaggcatg gattaaatta gctaccagtt ttttagtgac aacttccata acacgaagtt
    18901 cggttgcagt aaaatctgtt ttatcctttt gagcaccaaa ttggctattt ccgccaaaat
    18961 aataatcgac caagtcatac acaaaggtac tatcaaacaa aataattcct ttaccacgaa
    19021 gaggcttgaa tttgtagata cctatcaggc taggattagg taaactcttc ataaactcct
    19081 tatgctttgt aataagtagc ggatcttgtt taatttcaaa atctctagcc gttaaatgat
    19141 aaatatctgc tgcaaacaga cgaactgccc tgtcataaat tttgtctaat accggaagtt
    19201 gtcccttaac gatacgctct tgtccggtaa aattaagtgt tttgactcct tcttctgcta
    19261 cttttatatc agaatcaaaa gtggagttta attcctggct ggtagtatct tctggttttt
    19321 tacgagctgg ctgatcgctt gagttcaatt cctcatcatt cgtttcacca tcaattgact
    19381 catctactga atccagtaat gcatcaatct cttcttgcga taacacatct ttctcagtca
    19441 taagcaatcc attcgcatct caaccagatt tttcaaagtt attttaaaat tgaagggttg
    19501 attagtttaa aattagctca atccatcagt aacaactata gaatggttat gtttatgcaa
    19561 agcccatgcc atccatgaaa tatatcaata aatcaaatag atagtgtaat aatcaactct
    19621 tgattagctc atttaattcg actctgtcaa tgttttggca taaaaaatca tacgggttag
    19681 attgataggg tttacgaaaa actccaagat caagcaaaaa tgttttgagg agggagttta
    19741 tataaactaa atgactgaat taagaacatt ttttaacata gagattggga tttctcgtaa
    19801 gtcttatgca caatatctga ggttgtgagc attccttaat gttagtgtaa tcacttcaaa
    19861 aataaatgtt tagaaaatct tccacttagt ctactgcttg ttgctcgcat ttatcttcag
    19921 ctaaactttg acaattggag ttacaatcta tttcatcaga ggttaaacat acatcatcca
    19981 gacattgctg ataattttcc ccaacacatt cttccatgtt taatttatca gtaccctcat
    20041 ccagaccagg aatatccgca gcaaatgcta tcaaggaaaa aaataaaaag caggctatgc
    20101 tggcaagatt gaatattttg ttcattagaa taccttccat tgatatttga tttaattata
    20161 gacatcctat agcctttgtt cctgtttcca taataaaaaa atcatgcgac actcactcaa
    20221 ctgtctattt aaaaatttct gatatgcgta gcaaaagaat cttattaact aattttaccc
    20281 gcgcaattta tagtgaatcc tgttgtataa ggccagacac agaaaaagaa cttattcact
    20341 atattgcaca aaatcagcct aaaaacatgc taaccagggg tgcgggattg agttacagtg
    20401 atagctgtct aaatcaagac ggcctggtaa ttgatactag tcgctttaat catttcatcg
    20461 attttgatgt cgaaacaggc atcgtagtat gtcagggtgg aacaccattt aaggatctgt
    20521 ttttacttca tgatgaattc attcccccag ttctcccagg cacagcattt gccactgtag
    20581 caggtggaat agcccatgat gttcatggta aaaataacca ttcagcggga agttttggac
    20641 atcatatttc ctggtttgat ttattgatag gcgatcaaat catgcattgt agccgcgaaa
    20701 aaaatagtga tctatttttt gcaactatag caggtttagg tttaacaggc attattacac
    20761 aagtcgctat tcgtcttaaa aaagcaccac gccatctgac aataaaaaat aaaccatttc
    20821 catctatccg ggaattaatt gcagaaatgg cgacttatgg tcttcagcaa gattaccaag
    20881 tagcctggtt agacttgtta tattcagaac ccagagccat tttatcaaca gcaaattatt
    20941 gtgagtcgcc aaactatcct gatgaaaaat taaaaattta tagcctgcct aaattaccat
    21001 ttggacttat caaaaaatgg aacatgaaag tatttaatca atatttttac tccagacaaa
    21061 aagaaaatga aatgatgagt ctcatccaat acaataaccc tctggataaa atatcacatt
    21121 ggaatcgact ctacggccct agaggactta tacagtttca agccgtattt gatcaagata
    21181 aggccgggca aattattaat cacatattgg agattattag ggctcatagt gctactccaa
    21241 ccctcactgt ccttaaagta tttacacagc ctggattagg tttgctttcg ttttgtcggc
    21301 ctggatttac tctggctatt gattttatta ataatcctca cgccaaaaaa gcaataagag
    21361 cgatgaatca attcattgca gaaaacgatg ggtgtgttta tttggctaag gatttattac
    21421 taaatccaga acaatatttt acaatgtaca aaaatcaatc gaaatttgct caaatactta
    21481 caaaatatcg atgccccatg cgttcagatc ttgcgacacg tttaggaatc ataaaatgat
    21541 gcagcggaaa tgggttgttt taggagctac ttcagttatt gccgaacaat tcgcacatct
    21601 tgctgcgcaa gccggtaacc atttatgttt ggttgcacgc aattccgagc aattagcctt
    21661 aatctcaaaa gatattcaat tgcgatataa aaccccttgt gacgcgataa ctatcaattt
    21721 atccgagcct aacgagcaat tgcttaccat tttagaaacc agagaaagag aattggattt
    21781 atttattgcg cattgtgatt ttaccgataa taaccattta aatccgcaat cgattaaaca
    21841 tttaattgat accaatatct catctacagc gatcctgatt aatacctatt tacaatcaca
    21901 acaagaaaaa catcgtatcg tttatttaag ctccgttgct tcctgccgcg gccgtgccaa
    21961 aaatagtctt tatggtgcca gtaaagcagc aattgaaata tttctcgaag gcttgcaaca
    22021 aaaagcaact ccacatcaac atatcttaat cgttcgcctt ggatacattg atacaaaaca
    22081 aacgtatgga ctacccggag ttttttacgc agctcaacct catgactgtg caaaggcatg
    22141 ttgggaaaaa ataaatcata ataaaagatt attttactat ccttctttct ggagagtggt
    22201 catggctatt attacaaaac tccctttttt catttataaa agaatgggaa aaatataagt
    22261 attattatcg caatgattcg tttttatacg ttataacgcc cggttatagg cttttgtata
    22321 atgataaaaa ctatcctagt attaatttaa gtaatattat gattggtttt aaaataatga
    22381 atattgccga tttgcaatct tttcttgcag ttgtagaact ccactctatc tctttagctg
    22441 ccaaaaaatt gcatattact cagcctgctt taagtaaaag aattagaaaa cttgagtcgg
    22501 agtggaatac ccaacttttt atttcttcag gattaaggac tgagctaact gataaaggaa
    22561 aacaattatt gccttatgtt cggcagatgg tttatctgag ccatgagtta attagcaata
    22621 ctggcacaga taaaaaaaag cctcagttac tgctaaatat tggtaccaca gtatatattg
    22681 ctcaaactat tattcccccc cttatcatcc atatgaacgc actggacttg aactatttca
    22741 ttaatacgaa actcattgct gacaaagatg tagctcacgg tttaagtgaa ggtgcttatg
    22801 atctgattat ctccccgttc atgagtaatt ctgctgaaat taagtctgta cctttgtggc
    22861 gagaaaagct catcccaatt gtcgggttat cacatcctct ggcaaaaatt aaagaaaaac
    22921 tatcctttgc tgaattagca gaatttgatg cggtgcttat ggaaaaaaac tttataatac
    22981 gccgaattat tgataaagaa gccgaaaaac aaaagttgac tctcaaaata agagctgaag
    23041 ccaatatcat ttataataac atagctcttg tcgaacaagg tatagcttgg agtatgatta
    23101 atgaacgctt attgaatcct aatttaaaac ctcttgaact atctgattat gccctcagca
    23161 ttgattttta ttgtcatttt cttcagaaac gtacagatga acgattgatt cgtattttta
    23221 ttgataatct ggtcaattgg gttaacgcgt caagtgaatt aagctctttt tcccttgtta
    23281 attagataaa cactttattg ccaggttgca agaaacctct caggctgcac ttagcccaag
    23341 agatatcaat taaaactatt gatttaagtc agctttgcac tatctaagcc actgtattca
    23401 ttttcagtaa cttcgccttc ttcctcctct atcaaatagg cagatgaaga actattatta
    23461 ccaaaaattc ctaactgaat tctgactgag ttactgctgc ttgatgatgc tcctgattgt
    23521 tgggtagcat gagtacttac agcatcctca gagcgcgata ttactctaat ttcttctttt
    23581 agcttcgcat ggccatggtt cattttttta attttgcttg ttaattcaga aatttctata
    23641 ttcagttttt ctaattcttc cgttaatttt tctaactgtc tatttaattc ctttttatcc
    23701 ttctttttaa gagaggaatc cttcaattga gtttctacag catcaatctg ggctctcaac
    23761 atagatgctt gcagcatcaa acttttcata ggagccacaa ctattcttct ggattcactt
    23821 cccatatgcg ttaatttttc aacgatagta tgacgagtat tagccaagtg ctctaattta
    23881 tcggcatgcc tttctttaaa agggattccg ctaccaggat gcgcaataaa acattccagg
    23941 atgggtttta actgattagt aaattgtatt aatcccaaca aatcttccgc agggtataaa
    24001 ccaaactcat tagtaaaatg aggggccata ataatagcaa gattttccca tgtcattaga
    24061 ttggtttcat gaaaaacccc tgctgtatgc attaggtaac aatagtggta aagaatttct
    24121 ccaacacgtt gatgatcaag tatttttgaa agcaataaag agtcaataaa cttactcagc
    24181 aactgtacta ttgcgtcggc attttcaaca ttattcttct cagagctcaa tagatttttt
    24241 aattctgtca taaaaagctt gagcaatgag tcttcagttt tcaacaagac actttcttta
    24301 agaactaaag gcaacattcc taaaacatta ttcagatgta aattgtttat ctggccatct
    24361 tctaaaatat aacacgctaa tccccccaca ctaaaattgt cattaataac ttgctcaagc
    24421 aacttttctg actcttctct tgatccagcc agtcgaaata ctcctggcca gtttaatagc
    24481 tctgggtttt gtgacactaa ttgggcaaca catcgaaata cattgacaat cacatgagta
    24541 ctatatggtg gtggcataac aaattccccg attaaatttt gaacaaaata tcatgggatc
    24601 aaaactaaat caattattgt taatgaaata cgaacctgaa tttttccagc cacattaatg
    24661 tcttttggat taattgaatt catctcaagt gttacttgcg atgaaattta tgttccagaa
    24721 taaaatcatg attgtctaat caggaacggc ctaatagaat aggccgttcc tgattaattt
    24781 gaggaagcaa aaatcacttt aaaattttaa tcattggcaa tattgttttt attaccccaa
    24841 aaaattgcaa aatataaatg attaaaataa ttaataccaa tacatttaac agacttttaa
    24901 tagccccggg cataggtata aagacattaa ttaaccatag gcctacacca aaaaccacta
    24961 tcactgcgat taaattcagc aagtcattca taatagt
//
LOCUS       DAXLDT010000037        24759 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXLDT010000037 DAXLDT010000000
VERSION     DAXLDT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24759
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.037"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            98..1198
                     /locus_tag="ACF2SP_001162"
     CDS             98..1198
                     /locus_tag="ACF2SP_001162"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFD2358956.1"
                     /translation="MSLYIGLMSGTSMDGIDAALLELPSNQLIHGITKQYSDDVRRNL
                     DDLIIGNHLTLASICQLNTLIGREFAEAVRQLLIEIKVHPKEIQAIGSHGQTVCHDTS
                     GNIPYTLQLGCGHTISSLTGITVVADFRTRDLVNGGQGAPFAPLYHQQIFSKVNESVA
                     VVNIGGIANVTFIAKNQMTRGWDIGPGNCLMDAWIYKNKGALFDKNGVWASQGEVIHP
                     LLEYLLQDPFFHLDSPKSIGKEYFSLSWLQKHLKPDYTPADIQATLLALTAHTIAETI
                     LNESGEIKQLYLCGGGAHNTHLKESLARLLPGITVKSIAELGISPDYLEAMMFAWLAA
                     QTINQIPVNLASITGAKGIAILGAVYPIIKSY"
     gene            1254..2546
                     /locus_tag="ACF2SP_001163"
     CDS             1254..2546
                     /locus_tag="ACF2SP_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358957.1"
                     /translation="MTAHYNVASNSGARNTLATSYFIFFLAASFYLYEFILQVAPSVM
                     AESMMKTFGVTGQGFGVISAFYFYAYAPTQLPAGVLYDRYGPRKLMTFAIVLCAFGSA
                     FFASTDSVFTACIGRFLIGIGSAFSFIGVLVLISRWFPPYYFAILAGVAQLMSSVGAM
                     FGEMPLAALIDLVGWRSASFILAGVGFLLAILFWVFIRDYPHQQNQTIPDHYLRDEWK
                     RLVAVCKHAYTWIIGGYAFAIWTPIAVFAALWGVPFLQEKFQVSVVAASGLCSMIWLG
                     IGVGSPLLGWLSDKLESRRIALIISAVLGLIATLIILYWSELSYNWAYVVLFVLGLGA
                     GGQTVSFAVVKENNTPELVGTASGFNNLSVLIGGAIFQPLVGYILQQTGSWRIVNGAH
                     VYSISSYQTALLVMPVCFLASLLIATFLLKESHPARRG"
     gene            complement(3064..4344)
                     /locus_tag="ACF2SP_001164"
     CDS             complement(3064..4344)
                     /locus_tag="ACF2SP_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2358958.1"
                     /translation="MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKS
                     FNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHS
                     MNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
                     IIIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITKNY
                     KTLLSNQAFCYSAIAEGLVGIPCIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATIL
                     GNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYVYLIPGIIIYFFSLS
                     VINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANVFYQHHNNLHFALYCNA
                     VGLLFLIFIGLTFFIGTPKKSEIHDEANNNVANS"
     gene            4575..5741
                     /locus_tag="ACF2SP_001165"
     CDS             4575..5741
                     /locus_tag="ACF2SP_001165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD2358959.1"
                     /translation="MPKEKVVIIIPTYNEEDNISNTIDLILKETYSLADYQVDILVFD
                     SHSTDSTAHIVRELTYTSQRVHFAQEPQKTGLGSAYLQAMRIAINELNADVVFEFDAD
                     GSHQPCYIAPMLELLKQHDVVVGSRYISGGRIPSNWAFHRKLLSVGGNWVARMLLTGK
                     YKDLTSGLRAIRTNLLKKLLPSNFLSNNYAYKLELYWLLHKAKARIVEFPIQFIDREK
                     GKSKLPKNSMIDTLRVLFTLRFRELRQYLTMCCVGSIGALIQFSLYNWLRSSNHSALV
                     SSQVAITAAIISNFIFHHSITFKQKTDFRVTKTTFSRFTQFIIYSTFMVYFQSLWVHI
                     FVHSFGQGVLKENIIVASGLGIGSIINYLFYSNIIWSSKGAHSLNSNSSKETQI"
     gene            complement(5829..7202)
                     /locus_tag="ACF2SP_001166"
     CDS             complement(5829..7202)
                     /locus_tag="ACF2SP_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HFD2358960.1"
                     /translation="MNRYISLYFLLILLIAPIHLLGLDSYYYWDWSRHLALSYYDGSP
                     MIAYFIRIATLLFGDTLFALNLVTIVSTALTSFIIYKTARLCLNKEASYVTVLIWLFS
                     PLVTLDLINQTTYDTPLTLFWALTLYFTIRYIQFNRNQDLYYIGLSIGLMLLSKYSGI
                     VLVLALLIFLVATPYRRLFKSRHFYLALTLAIIVFSPVILWNAQHDWVSIRYQLSTHQ
                     TQNTHHAMGSVLHTFFSIFLPAINFLFIPPALYLSKGTPRKSPVFYLCLIICVTFGLF
                     YLFVAGKATIRGYWLSQYIITASLLAGYCYQEWAYRNSTYLLIVFYILTSGIILMDNT
                     TLFSFGYSKKLAYYQSIQQINQSNDKLPSIVITPGWFEARMLFFLKNKPTIFTFDCGS
                     MQNQYRYWSPNLNTLGEVLYIDTFDRLSCVKKYFAQCQSLNLPATFDEKGKPIIYAYW
                     CSNKLDT"
     gene            7313..8455
                     /locus_tag="ACF2SP_001167"
     CDS             7313..8455
                     /locus_tag="ACF2SP_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2358961.1"
                     /translation="MKLYFFGDDNNKPKLSEHLIIYILSLLDPKSQSVASLVNKDWSQ
                     LVQLTRQYLSMLPVIHRIPLFANLGLDVLKLKLMTGGMTNSTFRLRIKNAPEKWVMRI
                     PGIGSSAFITRRDEAHNAKQAEKLGLNVPIAFFDPEDGLQVTRFIEGVHSLDDKALAR
                     KEILHEVASMMRRLHKSPPFDNNTLFFERNEELLDLLKRKPFNFPGEIEFIEQQMKNL
                     KEIFSSYRIQQFPCHNDTTPLNFILSENPEIKGSEKIHQIDWEYSSNNDFIWDLAYFM
                     VEAKLNREQQLILLKAYFNTEELSGSLLAWIEVYKPIIEWWITIWSWTQLANGANAVD
                     LSSYEQLGLERYHNTLSHLKSEDYVKALALIEADSHSLTFTGHRLF"
     gene            complement(8508..9278)
                     /locus_tag="ACF2SP_001168"
     CDS             complement(8508..9278)
                     /locus_tag="ACF2SP_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFD2358962.1"
                     /translation="MTILVFDIETIPDIESGRRLFALDGLSDEDTACAMFALRREKAG
                     NDFLPHYLQKICAISVVISHGNQIKVWSLGDENSDEKELITRFFAGIDKHTPTLVSWN
                     GSGFDLPVLHYRSLYHSISAPTYWETGENQQTFKWNNYLSRFHYRHIDLMDVLAGYQN
                     RAFAPLDDISTMLGFPGKMGMSGSKVWEQYQAGQLKEIRDYCETDVLNTYCVYLKFEL
                     MRGTISQEGYDRNIAQLKNYLGAEKDKMHLQEFLKKML"
     gene            9524..10120
                     /locus_tag="ACF2SP_001169"
     CDS             9524..10120
                     /locus_tag="ACF2SP_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358963.1"
                     /translation="MKIRFTLLCFSIFLTGCVSKPPADINNICNIFKQYPKWYVDSKD
                     VERRWKVPVPVQMAIIHQESKFNARARPPRTKLLWIIPWTRPSTAYGYTQALRSTWDN
                     YKRTNGWFWSSRDDFGDGVDFIGWYANQANIKAGIPRTDAYSLYLAYHEGIGGYQRKT
                     YLKKPWLISVARKVKAKSQLYAMQLNSCQGSLKSRSWW"
     gene            10525..11181
                     /gene="adk"
                     /locus_tag="ACF2SP_001170"
     CDS             10525..11181
                     /gene="adk"
                     /locus_tag="ACF2SP_001170"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFD2358964.1"
                     /translation="MRLMLLGGPGAGKGTQASLLINRYKIPQISTGDMLRAAIAKGTP
                     LGLSAQKIMESGGLVSDDIIIGLVKERLKNPDCDRGFLFDGFPRTLVQAEALKDAEIH
                     LDHVIEIAVDDEEIIERISGRRIHQPSGRVYHVVNQPPKNPGVDDITGEPLIQRDDDK
                     EETIRKRLQVYHSQTAPLVQYYKEWAETGSKEAPKFHTISGTGTVEQIFDNIVTILET
                     "
     gene            11190..11552
                     /locus_tag="ACF2SP_001171"
     CDS             11190..11552
                     /locus_tag="ACF2SP_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFD2358965.1"
                     /translation="MPTQTINSAEFESLINDNEIVFIDFWAEWCAPCKQFSKVYEQVS
                     DQFPAIKFVKVNIEEQQELADFFEIRSIPHLMVFKDSIVIYSDAGSIPESTLKELAQQ
                     ALDADVSEIRAQLEQKDK"
     gene            complement(11512..13032)
                     /gene="glpD"
                     /locus_tag="ACF2SP_001172"
     CDS             complement(11512..13032)
                     /gene="glpD"
                     /locus_tag="ACF2SP_001172"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFD2358966.1"
                     /translation="MDQVFDVAIIGGGINGCGCAADAALRGLSVILFEQDDLASKTSS
                     SSTKLIHGGLRYLEHYEFGLVKKALQERQTLLNLAPHLVHSQSFVLPYLKHMRPSWLL
                     RLGLFFYDHLSRKNHLPKSRSIHRNNKNNYFTPLKEELNRGFLFYDATTDDARLTILN
                     AIQAKNHGASIRPHAKVTHAEVINNIWHLTIQPTKGQSYKAYAKSIINATGPWVQSIA
                     QLTHTPIQKDITLVKGSHIIVPKLFEGNHAYFLQHNDQRVIFVIPYHGFSMIGTTDIH
                     YTGDLNHIEIGEEEIDYLIELVNAYFKFKITKEDVVHSWSGIRPLLADDSKEVKSLSR
                     DYSYEFNHYPAPIITIFGGKITTYRQLAEEVVDQLTPVFPQMKPSKTQHTPLPGATLA
                     DMSFTQYVNYAKTKYHWLDDELLNRYLNTYGCYTEIFLSQCNNIEAMGKKYGPSLYQV
                     EVDYLILEEWANYCDDILNRRTKLGLTMDNVSKKELADYLSSISTYPSAQAELVFH"
     gene            13094..14569
                     /gene="glpK"
                     /locus_tag="ACF2SP_001173"
     CDS             13094..14569
                     /gene="glpK"
                     /locus_tag="ACF2SP_001173"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFD2358967.1"
                     /translation="MNYILAIDQGTSSTRAMIYTLQGDLIANSQYPLTQYYPNLGWVE
                     HDPEEIWQKTLNAMRDVVSQVQGKAILCCGLTNQRETTLIWNKKTGECLAPAIVWQDR
                     RTEEFCYSLSEYSSMIQEKTGLLPDPYFSASKLNWLLKNNAQAQSLINQGDLAFGTID
                     SFLIWRMTKGASHATDITNASRTMLFNIHDEKWDKQLLELFSISESILPEVYSSDNHF
                     GVIDKEWLGVEIPITGVAGDQQAALIGQGCFNGGMVKATFGTGGFLLLNTGDKPVFSK
                     HKLLTTIAYKIKEKTAYGLEGSIYHAGTTVKWLRDEMKLISHSAETEVLAQSLDSNNG
                     VYLVSSFTGLGAPHWLSITGTAIIGVSRSTNRAHFARAALEGVCYQTRDVLTCMRDDS
                     QLDLTLLRADGGMAVNQWFLQFMADQCQLTIQKPKDIETTAKGAAVLAAIGSGLFDSV
                     SSVNKVWDVENEFQPSREREVVEKDFRGWLEALAMVKARHA"
     gene            complement(14663..15934)
                     /gene="gltA"
                     /locus_tag="ACF2SP_001174"
     CDS             complement(14663..15934)
                     /gene="gltA"
                     /locus_tag="ACF2SP_001174"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFD2358968.1"
                     /translation="MTQKTAKLSIDGQETIDLPIYTPTLGNDVIDITQLGSHGVFTFD
                     QGFLSTASCESKITYIDGDKGILLYRGYPIEQLAEKKDFLDVSYLLLNGELPNKEEKK
                     AFVSLINNHTMVHQQMYQFLNGFRRDAHPMAIMVGIVGALSAFYHESMDLNNAQDRFI
                     SAIRLVAKMPTLAAMSYKYSIGMPYMYPKNKMSYAENFLHMMFGVPSEDTTPDPVLVR
                     AMDTIFILHADHEQNASTSTVRLAGSTGANPFACISAGIGALWGPAHGGANEACLNML
                     KEIGDVSRIDHYIKRAKDKNDPFRLMGFGHRVYKSYDPRAKVMRKTCYDVLEAVGAKE
                     APLFKLAMELERIALEDEYFIEKKLYPNVDFYSGLTLSAIGIPTNMFTVIFALARTVG
                     WMSHWMEMVATKSRIGRPRQLYTGEKIRDVK"
     gene            16160..17041
                     /locus_tag="ACF2SP_001175"
     CDS             16160..17041
                     /locus_tag="ACF2SP_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HFD2358969.1"
                     /translation="MITPAELPINHRGAVYHLDLRTEEIADLIITVGDPGRVSLVSQH
                     FDKIEVQRSHREFVTHTGYLGTNRITVLSTGIGMPNIDIVMNELDALANIDLSTRTVN
                     TNKKSLTIIRLGTTGGVTANCMPGDIAISRYAIGFDSLIDYYQYQPMGVLETIREELS
                     QYLMGESGPFYISESCPQLAEQFSALGHTGITATCCGFYGPQGRQLRLPLHYPNLITQ
                     LGKFCTQNYSVINLEMETAAIYALGSMLGHKCLSLSVVINNRIHGSFVTDVKSCLDSL
                     ITKSLANIKNILEPYEA"
     gene            17125..19716
                     /gene="gyrA"
                     /locus_tag="ACF2SP_001176"
     CDS             17125..19716
                     /gene="gyrA"
                     /locus_tag="ACF2SP_001176"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFD2358970.1"
                     /translation="MVYLAKEVLPVNIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYLL
                     IDGQGNFGSVDGDAPAAMRYTEVRMSKVAHALLADLDKETVDFSPNYDETEFAPVVLP
                     SRIPNLLVNGSSGIAVGMATNIPPHNLTEVINACIALVDEPDTSLEDLMEIIPGPDFP
                     TAAIINGRAGIIEGYRTGKGRVVIRARTEIETDESSGRQSIIIQELPYQVNKARLIER
                     IAELVRDKKVEGISGLRDESDKQGMRVVIELKRNEVADVVLNNLFAHTQMQNVFGINM
                     VALVDGQPRTLNLKQILEYFIKHRREVVTRRTIFELKKARSRAHLLEGLGIALANIDE
                     MIALIKQSPTPQDAKSALLSKIWQPGLVKAMLEKAGSNASRPDDLTEEYGLHENGYKL
                     SEAQAQAILELRLHRLTALEQDKIINEFEELLNLIKELLDILASPERLMQVIRDELIE
                     IKSQFGDERRTEITASQEDLTIEDLITEEDVVVTLSHQGYVKYQPITAYQAQRRGGKG
                     KSATHVKDEDFVERLVIASTHDTLLCFSNHGKLYWLKAYQLPQASRASRGRPIINILP
                     LAEGEEINAMLPVREYKDGSYVFMATKKGTVKKVPLNAFSRPRSNGIIAVDLEEDDSL
                     VGVDITDGTRDIMLFTDAGKVIRFDENKVRPMGRTARGVRGIRVEKDQAVKSLVVVDP
                     NGGTILTATENGYGKRTHIDEYRVSGRGGQGVISIQVTERNGKVVRSLQVTDNDEAML
                     ITDKGTLVRFKVNELSVIGRNTQGVRLINVSSGETVVGMQKIVDLGEELEEAEDSSLN
                     AEDNSDE"
     gene            19709..20797
                     /gene="serC"
                     /locus_tag="ACF2SP_001177"
     CDS             19709..20797
                     /gene="serC"
                     /locus_tag="ACF2SP_001177"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFD2358971.1"
                     /translation="MNSRVFNFGAGPAMLPEEILKEAQEEFLNWRNTGMSILEIGHRT
                     PEIINLLSTAEQSLRELLNIPKNYHVLFLGGAARAQFAMIPMNLLQPGDEAAYFITGI
                     WSKMAYHEANLLKQAYYLSNEEKEGFVSIPDYQKWELKSNTPYVYYTPNETINGVRFP
                     YVPKTGGVPLVADMTSCLLSEPININQYGLIFAGAQKNIANAGLTIVIIHEDLLKNQP
                     EPAIPTMLNYKNHAEHRSLYATPPVFNCYLASKMFEWIKTQGGIEGVFQRNCLKAAKL
                     YQYLDSTDFYLTPVSKEARSIMNICFSLCYPDLEQKFLDMANKRGLKALKGHRFTGGL
                     RASLYNAMPMAGVDALIEFMSEFAKENG"
     gene            20803..22104
                     /gene="aroA"
                     /locus_tag="ACF2SP_001178"
     CDS             20803..22104
                     /gene="aroA"
                     /locus_tag="ACF2SP_001178"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFD2358972.1"
                     /translation="MLNFISKPVGCLKGEITVPGDKSISHRSIIFGAIAIGTSVIDGF
                     LDGEDCIATLKAFQSMGVRIEGPDKQRVIIHGVGKYGLKQPQNIIDCVNSGTSMRLLA
                     GLLAAQQFDSQLTGDESLLKRPMLRISRPLSQMGADVTTQDGKPPIVIKGGKKLNGIH
                     YVMPEASAQVKSCLLLAGMYAEGQTKITENAVSRDHTERMLRTFSYPVQIQDGAIVID
                     RNGECHGTRLNIPGDISSAAFFIVAASITPGSDVLIRNVGINPTRTGIIHILTEMGAD
                     IRVLNQRAYGEEPVADLHIRYSQLKGIDIPASMVPLAIDEFPVIFIAAACAQGKTTLH
                     GAKELRLKESDRIGAMVDGLNQLGVHAEGFDDGILIEGGSIQGGEVNSRGDHRIAMSF
                     AIAGAVASAPVTIKNCANVATSFPSFVTTANMLHFQIEEYS"
     gene            22104..22796
                     /gene="cmk"
                     /locus_tag="ACF2SP_001179"
     CDS             22104..22796
                     /gene="cmk"
                     /locus_tag="ACF2SP_001179"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFD2358973.1"
                     /translation="MVFNNEVPVITLDGPSGTGKGTICHLVAKRLHWNMLDSGAIYRV
                     LAYAARKNSIEPNDIKKLTALARSLNLRFESSADNESKVILDDENVSQQIRSEQCGQD
                     ASQIAVIPEVRAALLERQRNFAQFPGLVTDGRDMGTVVFPNAVLKVYLYASAEERANR
                     RYLQLQDNGINVSLAQVVEELAKRDARDTARTHAPLKPAEDAVLIDTTGLTIVQVFNI
                     VLKLIDERLNNL"
     gene            22862..24538
                     /gene="rpsA"
                     /locus_tag="ACF2SP_001180"
     CDS             22862..24538
                     /gene="rpsA"
                     /locus_tag="ACF2SP_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFD2358974.1"
                     /translation="MSESFKELFEQSIAGAQFYPGAIITAKVIDIDDDFVTLNAGLKS
                     EGIVAVEEFYDKNGELEVKVGDTVEVALDSVEDGHGETLLSREKAKRQEAWRKLSKCH
                     ENNETVTGLISGKVKGGFTVEIGSIRAFLPGSLVDVRPVRDPSYLEGKELEFKVIKMD
                     LKRNNIVVSRRAVVEEESSADRQALLESLHDGQELHGIVKNLTDYGAFIDLGGIDGLL
                     HITDISWKRVKHPSEVLSVGQDVKVKVLSFDSERNRVSLGMKQLGNDPWVDLVERYPI
                     GKRLQGKVTNITDYGCFVEIEEGVEGLVHMSEMDWTNKNVHPSKVVSLGDVVDVMVLE
                     IDEERRRISLGMKQCVGNPWQQFASTHNKGEKVKGKIRSITDFGIFIGLDGDIDGLVH
                     LSDISWTVPGEEAVKQFKKGQELEAVILAIDPERERISLGLKQLEGDSFASFAETYTK
                     GSIVKGTVTAVEPKTVTVALAEDVSGTIRVSELSDERVDDATTIVKVGDEVEAKITNI
                     DRKNRTISLSVKAKDAQDEADAIKKYSRTEAASTTLGDLLKEKMASKEGE"
     gene            24652..>24759
                     /locus_tag="ACF2SP_001181"
     CDS             24652..>24759
                     /locus_tag="ACF2SP_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HFD2358975.1"
                     /translation="MRILMLVTYLLLIIIGVSFAALNASSVQVNFYFKTV"
BASE COUNT         7616 a         4289 c         5235 g         7619 t
ORIGIN      
        1 gtagttctca atttcaaagc agaagcagta atttttttgc ctgtgaataa aggttttagt
       61 agtattgata tatcctattg caaatttaga gtaattaatg agtttatata tcggtttgat
      121 gtcaggtacc agtatggatg gcattgatgc tgccttgctg gagcttcctt ccaatcaatt
      181 gattcacggg attactaaac aatacagtga tgatgtcagg agaaatcttg atgatttaat
      241 tataggtaat catttaaccc tggcttcgat ttgccaactg aacacgctaa ttggcaggga
      301 gtttgcagaa gcagttcgcc aactcttgat tgaaataaaa gtgcatccaa aagaaattca
      361 ggccattggt agtcatggtc aaactgtctg tcatgataca agtggaaata tcccatatac
      421 tttgcagctt ggctgtggcc atacgatttc ttctttaact ggcattaccg ttgtagcaga
      481 ttttagaaca agagatttgg tgaatggtgg tcaaggtgcg ccttttgctc ccctttacca
      541 tcaacagata ttttctaaag tgaatgagtc tgttgctgtt gttaacatcg gtggtattgc
      601 taatgttacc tttattgcaa agaatcaaat gactagaggt tgggatatag ggcctggcaa
      661 ttgtctgatg gatgcctgga tttataaaaa taaaggagcc ctttttgata aaaatggtgt
      721 ttgggcgagc caaggtgaag tgattcatcc tctacttgaa tatttgttac aagatccatt
      781 tttccatttg gattcaccta aaagcatagg caaagaatat ttttctttat cctggctaca
      841 aaaacattta aaaccggatt acacaccagc tgatattcag gctactttgt tggcgcttac
      901 agcccatacg atagctgaaa caatattgaa tgaatcagga gaaattaaac aattatatct
      961 ctgtggaggc ggggcccata atacgcattt gaaagagagt ttagctcgtc tactacctgg
     1021 gataacagta aaaagcatag cagagttagg gattagtcct gattatcttg aggcaatgat
     1081 gtttgcatgg ttagcagcac agacgattaa tcaaatacca gttaatttag catcaattac
     1141 tggagctaaa ggtattgcaa ttttaggtgc agtgtatcct ataataaaat catattgaca
     1201 aactaaagaa tcataaggtt taatttcata aaaattaaca gagtgatttt aaattgaccg
     1261 cacattacaa cgtcgcttca aacagtggag caagaaatac acttgcaacg tcttatttta
     1321 ttttttttct agccgcttca ttttacttgt atgaattcat actacaagta gcaccaagcg
     1381 ttatggcaga gtcgatgatg aaaacttttg gggtgacggg tcaaggcttt ggtgtcattt
     1441 cagcttttta tttttatgca tatgccccta cacaattgcc agcaggagta ttatacgata
     1501 gatatgggcc acgcaagtta atgacttttg caatcgtttt gtgtgcattt ggttctgcct
     1561 tttttgcttc cactgacagt gtatttacag cttgcatagg ccgatttttg atcggaatag
     1621 gctctgcgtt ttcttttatc ggtgttttgg tccttatttc acgctggttt cccccttatt
     1681 attttgcaat tctagcaggg gtagcgcagt tgatgagttc agtcggcgct atgtttggtg
     1741 aaatgccgtt ggcagcttta attgatctgg ttggatggag aagtgcgagt tttattttag
     1801 cgggtgttgg atttcttttg gctattttat tttgggtatt tattcgagat tatcctcatc
     1861 agcaaaatca gacaatccct gatcattatt taagagatga atggaagcgc ttagttgctg
     1921 tctgtaaaca tgcttataca tggattatcg ggggttatgc ttttgcaatt tggactccta
     1981 ttgcagtatt tgctgcactt tggggggttc cttttttaca ggaaaaattt caagtaagcg
     2041 tagtggctgc ctcaggctta tgcagtatga tttggctagg tattggtgtt ggtagtccct
     2101 tgttaggttg gttgagtgat aaattggaga gtcgtcgtat tgcattgatt atcagtgcgg
     2161 ttttagggtt gattgcaact ctgattatcc tttattggtc tgagttatct tataattggg
     2221 cttatgttgt cttgttcgtt ttaggattag gtgctggagg acagacggta agttttgcag
     2281 tagttaaaga aaataatacc ccggaattag ttggcacagc atctggtttt aataatttgt
     2341 ctgtattaat tggtggggcc attttccagc ccctcgttgg ctatatcttg caacagacag
     2401 gatcatggcg aattgtcaat ggtgctcatg tatacagtat atcgagttat cagactgcgt
     2461 tgcttgttat gccagtatgc ttcctggcca gtttgcttat tgccactttt ttgcttaagg
     2521 aatcgcatcc tgcgcgcaga ggttaataac agatttatca attcaatatt ctagccccga
     2581 tatctttttg cttaggctta aataatgtaa tgtgagttat gggtaaagat tatataggag
     2641 ccagtcgata tccggaatcc gttcaggctg tggagatgcg taagtatcgt ctcgaagctt
     2701 tggcacgaga cattaaattc catacaaggc ttcgtgacgg cataaatgcc tcctcagcct
     2761 gaacggcttg agataacagc tgctcttact cataacttac gttaatataa ttggagtctt
     2821 ggtagtgtgc ggataaagcg agctaagaaa atattcagct aaaatttgct gtaatctgtt
     2881 ggtaattgag agacctaaat tcttatatta gactttttga gcaggctgcc tctttagtct
     2941 aggtgtcgtg ttgcaaaagt tttaatatag caacaatttg atacatgtta actccattca
     3001 cctgttcatg tttgcgttgt taacctaaaa caaaggaatc ggttatgtaa atgtggcctg
     3061 ttcttatgag ttagccacat tattatttgc ttcatcatga atttcagact tcttgggagt
     3121 ccctataaaa aaagttaaac cgataaagat taaaaataat aatccaacgg cattacaata
     3181 aagggcgaaa tgcagattat tgtgatgttg ataaaacacg ttagctattt ctattcctat
     3241 agccccaatg atcataatac ttaggctgat taatgccgag gctgttcctt tgcttacagc
     3301 ggttacaaat aaacaatacc tgttcaaggg ggcattaatt acactcagtg agaaaaagta
     3361 aattatgatt cccgggatca aatacacata attattgcca taaaaataag ggagaagagc
     3421 tgtcagagca agtcccacga ccattatgat acaacctata aaaataattc tctcgatttt
     3481 atatttgtaa gttaaatgat gtaagcacca gtttcctaaa attgttgccc cgaagatggg
     3541 tagttgccaa aggccatatt gaattacagt taattttgct tcagcaatta atatgattgg
     3601 cgccagcgca atccaggcga tgcatgggat ccctaccaga ccttcagcaa ttgcactata
     3661 gcaaaaggcc tgattagata ataacgtttt ataatttttg gttatagcgt tcagtgcgaa
     3721 cggtgtttta ggtataagct ggccatctct ttttacctgg cctatgggtt ctggcataaa
     3781 ccgccataat ccccaataag ctaaaagagc acccaaagca ataataataa aaatcaaacg
     3841 ccaggacgca taatgaatga ttatggctcc caatagtggc cctaataatg gggctaatat
     3901 tgcggcatta gccatgatag caattaatcg gatagcgtcc atttcttcaa atatttcttg
     3961 aattgtggca tagcctataa cgccaatgaa acacaatccc attccctgaa aaaaccgggc
     4021 gattaaaaac tgattcatgg agtgtgagct tgcaatcaaa agagtgaaca gaaaaaataa
     4081 gcatgatcct attaacatca taggtcttct gccataagcg tctgatagtg ggccaagtat
     4141 gagctgcaaa ctggcccctc cgagaacata gacagttaga gaggtggcaa caacactttc
     4201 gtgcgcgttg aatgatttaa caacattgat catacctggc atgatcatgt cattggctat
     4261 ataagtcaaa aattcatata aaaccaggaa gcaagcaaag ataatggcct gtctgtatga
     4321 aattttaatt agtggctcag acatagcatt aaccctgaat caatcgtttc taaaaggcgg
     4381 tctgtcagta tatacgttta tttcataatt tggtagaaaa tctgataaat gttttggtaa
     4441 gtgacataaa atctgccata tactgagaat aaatccttac aatagcaact gtcacttaaa
     4501 gtttgtttac ggcaagtcca ccatttgata agatttgctt acttctaaaa tctgcttctt
     4561 atgatctgat cagtatgccc aaagaaaaag tcgttattat tatccctacc tataatgaag
     4621 aagataatat ttctaatacg attgatttga ttcttaagga gacttactca ctcgcggatt
     4681 atcaggttga cattcttgta tttgatagtc actccacaga ttctacagcc catatagttc
     4741 gagagctaac ttatacctct caacgtgttc attttgcaca agaaccacag aaaactgggc
     4801 ttggttccgc ttacctgcaa gctatgagaa ttgcaataaa tgagttaaat gcagatgttg
     4861 tatttgagtt tgatgcagac ggttctcatc aaccttgtta tattgctcca atgttagagc
     4921 tacttaaaca gcatgatgta gttgtgggga gccgatatat ttcaggggga aggattccct
     4981 caaactgggc attccatcgt aaactattat ctgtgggagg taattgggtt gctcgtatgt
     5041 tattaactgg aaaatataaa gatcttactt caggtttaag ggcaatacgt accaatctgc
     5101 taaagaaact attgccttca aacttcctat ccaataatta cgcgtataaa cttgagttat
     5161 actggctttt acacaaggca aaagccagga tcgttgaatt tcctattcaa tttattgata
     5221 gagaaaaagg gaaaagtaaa ttacctaaaa acagtatgat tgacacccta cgtgttcttt
     5281 ttacccttcg cttcagagag ttacgccagt acctaactat gtgctgtgtt ggctccatcg
     5341 gtgcattgat tcaattcagc ttatacaatt ggttgcgcag ttcgaaccat tccgctctgg
     5401 tatccagcca agttgctatc actgctgcta ttatatccaa ttttattttt caccattcca
     5461 ttacattcaa acaaaaaact gacttccggg taactaaaac aacgtttagc cgttttacac
     5521 aatttattat ttattccaca tttatggttt attttcaaag cctatgggtt catatttttg
     5581 tccattcctt tggacaaggt gttttgaaag aaaatatcat tgtagcctca ggcttgggca
     5641 ttggatctat tatcaattac ctgttttatt caaatattat ttggtcatca aaaggtgctc
     5701 attccctcaa ttcaaactct tctaaagaaa ctcaaatttg aaagcataag tgtaatgaga
     5761 ttagataaac taaaccctca ataaaagcat tttttacaaa gagattagcg gtataagtcc
     5821 cgtttcattt aagtatccag cttattactg caccaatagg cgtaaatgat tggtttacct
     5881 ttttcatcaa aagtagctgg caaattcaat gattgacact gggcaaagta ttttttaacg
     5941 catgataatc tatcaaatgt atcgatatac agcacttcgc caagggtatt cagattaggt
     6001 gaccaatacc tgtattgatt ttgcattgaa ccgcagtcga aggtaaaaat tgttggttta
     6061 tttttaagaa agaacagcat gcgtgcctca aaccatcctg gcgttatgac tatgcttggg
     6121 agtttatcat tagactgatt aatttgttgt atcgattgat aataagccag tttttttgaa
     6181 tacccaaaac tgaaaagagt ggtattgtcc atcaaaatga ttccactggt aagaatgtag
     6241 aatacaatta ataaataagt agaattacga taagcccact cttgatagca ataaccagct
     6301 aataagctgg cagtaattat gtattggctc aaccaatatc ctcttatcgt ggctttgccg
     6361 gcgacaaata aatagaaaag accaaatgtt acgcaaataa tgaggcacaa ataaaataca
     6421 ggggattttc taggcgtccc ttttgataga tatagcgctg gtggaatgaa tagaaagtta
     6481 attgcaggga ggaaaatact aaaaaaggta tgcaagacac tccccatcgc atgatgagta
     6541 ttttgcgttt gatgagtact taactggtag cgaatggaga cccaatcatg ctgtgcattc
     6601 catagaataa ctgggctaaa gacaatgatt gccagggtta atgccaaata aaaatgcctg
     6661 gatttaaaaa gccgtctata gggtgttgca accaaaaaaa taagtaaggc cagcacgaga
     6721 acaatacctg agtatttgga taaaagcata aggcctatac tcaagcctat gtaatacaaa
     6781 tcctggtttc gattaaactg gatatacctt attgtaaagt aaagagttaa tgcccaaaaa
     6841 agggttaaag gtgtgtcata ggtagtttga ttgatcaaat ctaaagtgac taatggtgag
     6901 aataaccaaa tcaaaaccgt aacataactg gcttccttat taaggcataa tcttgctgtt
     6961 ttataaatta taaaactggt caaggcagta gaaactatag tcaccaggtt taaagcaaag
     7021 agagtatctc caaataaaag tgtggctatg cggataaagt aggcaatcat tggagaacca
     7081 tcatagtatg atagagccaa atgcctgctc caatcccagt aataataact atctaatcca
     7141 aggaggtgaa tcggtgcaat taatagaatg agtagaaaat agaggctaat gtagcgattc
     7201 atgtgcaaaa acattcagta ttccctggaa agtaatgatg aattgtatgt tattttatta
     7261 tcagttttta tataatgcat tcagttttat cattaattaa taaatgacgt ttatgaaatt
     7321 atattttttt ggtgatgata ataataaacc caaattaagc gaacatttaa tcatttacat
     7381 actcagtttg ctagatccta agtctcagtc tgttgcttct ttagtgaata aagactggtc
     7441 tcagctggtt caattgacac ggcaatacct gtctatgtta ccggtgattc atcgaatacc
     7501 tttatttgcg aatttgggtc ttgacgtttt gaaattaaaa ttaatgacag gtggtatgac
     7561 gaattccaca tttaggctaa gaatcaaaaa tgcgcctgag aaatgggtta tgcggattcc
     7621 tggaataggc tcttcagcat ttataaccag aagagatgag gctcacaatg ctaaacaagc
     7681 cgaaaaactt ggactgaatg ttccaatagc tttctttgat ccagaagatg ggctgcaggt
     7741 aacacgcttt atagaaggag tgcattctct ggatgataaa gctttggccc gcaaggaaat
     7801 tctgcatgag gtagcatcta tgatgagaag acttcataaa tctcctcctt ttgataataa
     7861 cacgcttttt tttgagcgaa atgaagagtt attggattta ttaaaacgaa aaccctttaa
     7921 tttcccaggg gaaatagaat ttattgagca acaaatgaaa aatctaaagg agattttttc
     7981 ttcttatcgt attcagcaat ttccttgcca caatgataca acgccactta attttatctt
     8041 gtccgaaaat ccagaaatca agggttctga aaagattcat cagattgatt gggaatattc
     8101 aagtaataat gattttattt gggatttggc ttattttatg gttgaggcca agctaaaccg
     8161 tgaacaacag cttattctgt tgaaagctta ctttaataca gaagagctga gcggctctct
     8221 gttggcatgg atagaggttt ataaacccat cattgagtgg tggataacca tttggtcatg
     8281 gacacaattg gcaaatggag ctaatgcagt tgatttgagt tcttatgagc aactagggct
     8341 tgaacggtat cataacacct taagtcactt gaaaagtgag gactatgtca aagccttggc
     8401 attgattgaa gcagatagtc atagccttac ttttacgggg catagacttt tttagcggtc
     8461 gccattatct agacaggaca actcgtttat agtgacgatt aagatcttca gagcattttt
     8521 ttgagaaatt cctgtaaatg catcttgtct ttctcggcac ctaaatagtt tttgagttgt
     8581 gctatgtttc tgtcataacc ttcctggcta attgtgccac gcatcagttc aaattttaaa
     8641 tacacacaat aggtgttaag tacgtctgtt tcacaataat ctcttatttc tttcaattgg
     8701 cctgcctggt attgttccca aactttagag ccactcattc ccatttttcc agggaaacct
     8761 aacatggttg aaatgtcatc aagaggggca aatgctctgt tctgatatcc tgcaagaaca
     8821 tccattagat caatatgtcg gtagtgaaaa cggcttagat aattgttcca tttaaaggtt
     8881 tgttgatttt cccccgtctc ccaataagtg ggtgcactaa tactatggta tagtgatcgg
     8941 taatgcagta caggaagatc aaaaccgctc ccattccagc tcaccagggt gggtgtatgt
     9001 ttgtcaattc cagcaaaaaa acgggttatt aattcttttt catcagaatt ttcatcgcca
     9061 agagaccata cttttatttg atttccatga ctgattacca ctgatatagc gcagattttt
     9121 tgtaagtaat gaggcaaaaa atcattgcct gctttctcgc gccttaaggc aaacatagcg
     9181 catgccgtat cttcatctga taagccatca agtgcaaaaa gccttcgtcc tgactcaata
     9241 tctggaattg tttcaatatc aaaaacaagt attgtcataa tttgttggcc cagttcggtc
     9301 ttaattctta tttttgcaaa ttttcttgaa actatcgaca agtctcagat ttgtcaaaat
     9361 cacaattggg gtatcagatt gattctatca tgtgaaggaa tagaagtaac ttcaaatggc
     9421 atagattgta tgcagtatat ttgatacaat attttttaaa ttgatacctt atatcgtagc
     9481 attgatattc aatactgcag gatgagtttt ttatcattaa atcatgaaga ttcgatttac
     9541 tttactttgt tttagcattt ttcttacggg ctgcgtcagc aaacctccag cagacattaa
     9601 taatatttgt aatatattta aacaatatcc gaaatggtat gtggattcaa aagatgttga
     9661 gagacgatgg aaggttccgg ttccggtcca aatggcaatt attcatcaag aatcaaaatt
     9721 taatgctcga gccaggccac caagaaccaa actgttatgg ataattccat ggacaaggcc
     9781 atcgacagct tacggatata ctcaagcgtt gcgttcgact tgggacaatt ataaacgcac
     9841 caatggatgg ttttggtcat cacgcgacga ttttggagat ggtgttgatt ttataggatg
     9901 gtatgctaac caggcgaata ttaaggcagg tattccccga acagatgctt actcacttta
     9961 tttggcttat catgaaggga taggcggtta tcaaaggaaa acctatttga aaaaaccatg
    10021 gttgatttct gtagcgcgca aagtgaaggc taaatcacaa ctttatgcaa tgcagctcaa
    10081 ttcttgtcaa ggttcgttga agtcacgttc ctggtggtaa ttaagatggc ctaattatgt
    10141 tcttcccagt ggaaataagg tcagacttgg aacatatatg attttgtgtg tttataataa
    10201 tcgccataac acgaagtcaa tcttaagtaa ggtatacatt tctattttta aatagaaaat
    10261 cgggtaaaat cctgtttatt taacagaata aagcttgaaa agaaaaggtg agtgatggtc
    10321 tgcttatgat gtgcgcactc aatccttata atagataata gtcctaaaaa aactgttttt
    10381 tgagctgctc atttagtttt ttaagactat gcatgaccaa aattatttgt caatgtgcac
    10441 taaataatta taaaatttta tagtgctact atgttactat attttaaagt atataatgat
    10501 tatgaattat ttaaggagtt ttggatgcga ttaatgcttt taggtggacc gggagccggc
    10561 aaaggaaccc aagcttcact tttaattaac cgttataaaa ttcctcaaat ttctaccggt
    10621 gacatgctgc gtgctgccat agcaaaaggt acgcccttag gattaagtgc ccaaaaaatc
    10681 atggaatcag gtgggttggt gtccgatgat attattatcg ggttagtgaa ggaaagacta
    10741 aaaaaccctg attgtgatag aggattcctg tttgatggtt ttccaagaac attggtccag
    10801 gccgaagctt taaaagatgc agaaatccat ttggatcatg tgatagaaat agccgttgat
    10861 gacgaagaaa taattgaacg catcagcggg cgacgcattc accagccttc tggtagggtt
    10921 tatcacgttg tgaatcaacc gccgaaaaat ccaggtgtag atgacatcac aggtgagcct
    10981 ctgattcaac gtgatgatga taaagaagaa accatacgta agcgcttgca ggtttaccat
    11041 agtcaaacag cgccattggt gcaatattat aaagaatggg ctgaaactgg ctctaaagaa
    11101 gctccaaaat ttcacactat tagtggtaca ggtacagtag agcaaatatt tgacaacatc
    11161 gtaacgatac tcgaaactta ggagttaaca tgccgaccca gactattaac agcgctgaat
    11221 ttgaatcatt aattaatgat aatgagatag tttttattga tttctgggct gagtggtgtg
    11281 ctccctgtaa gcaattttca aaagtatatg agcaagtttc cgatcaattt ccagctatta
    11341 aatttgttaa agttaacatt gaagagcaac aagagttagc agattttttc gagatccgat
    11401 ccatccctca tcttatggtt ttcaaagaca gtattgttat ttattctgat gctggaagca
    11461 tacccgagtc cactttaaaa gaattggcgc aacaagcact tgatgctgat gttagtgaaa
    11521 tacgagctca gcttgagcag aaggataagt agatatacta gacagataat ccgcaagttc
    11581 ttttttactg acattatcca tagtaaggcc gagcttagtg cggcggttaa gtatgtcatc
    11641 acaataattc gcccactctt ctaaaataag ataatctact tccacctggt agagtgatgg
    11701 gccatatttt ttccccattg cttctatatt gttgcattgc gaaagaaaaa tttcagtata
    11761 acaaccatag gtattgaggt aacgatttaa tagttcatca tccagccaat gatattttgt
    11821 tttggcgtaa ttgacatatt gagtaaaact catatccgct aaagttgccc ccggtaaggg
    11881 cgtgtgttgt gtttttgatg gtttcatttg tggaaataca ggggtaagtt gatctacaac
    11941 ttcttcagcc aactgcctgt aagtggttat ttttcctcca aaaattgtaa taatgggagc
    12001 agggtagtgg ttaaactcat aggaatagtc acgacttaat gatttgactt ccttgctatc
    12061 atcagctaat aaaggcctaa taccactcca tgaatggact acatcttctt tagttatttt
    12121 aaacttgaag taggcattta ccaattcaat taaatagtca atttcttctt cgccaatctc
    12181 tatgtggtta agatctccag tatagtggat atcggtggtt ccaatcatac taaagccatg
    12241 ataggggata acaaaaataa cccgctgatc attatgttgc aagaaatagg catgatttcc
    12301 ctcaaaaagt ttggggacga taatatggct acctttaacc agggtaatat ctttttggat
    12361 aggtgtatga gttaattggg caatggattg cacccatggt cctgtggcat taatgatgct
    12421 tttagcataa gctttatagc tctgcccttt ggtgggctgg atagttaaat gccatatgtt
    12481 attgattacc tcagcatggg ttactttggc atgtggcctt atgcttgcgc catgattttt
    12541 ggcttgtatg gcattaagaa tagttaagcg agcatcatcc gtggtggcat catagaaaag
    12601 aaaaccgcga tttagctcct cttttagcgg cgtaaaataa ttattcttat tatttctgtg
    12661 gatcgatctg gatttgggta aatgattttt tcgactgaga tgatcgtaaa aaaataaacc
    12721 taacctcaat aaccatgagg ggcgcatatg ttttaagtag ggcaaaacaa aagattggga
    12781 atggactaaa tggggagcca aattgagaag cgtttgacgt tcctggagtg cttttttgac
    12841 taaaccaaat tcataatgct ctaaatacct taaaccacca tgaattaatt ttgtgctact
    12901 tgatgaggtt ttcgaagcca gatcgtcctg ttcgaagagt atcacagaca gacctcttaa
    12961 tgccgcatct gcagcgcagc cgcagccatt gatgcctcca ccaataattg caacatcaaa
    13021 aacctgatcc atgtttttat ctccgaaaaa agattacact caatatatat aaatatagag
    13081 ggattttata ataatgaact atatactagc tattgaccag ggaacaagta gcactcgcgc
    13141 tatgatctac acccttcaag gtgatttaat cgcaaatagt cagtatcctc ttactcaata
    13201 ttaccccaat ttgggttggg tagaacacga tcctgaggaa atatggcaaa aaactctgaa
    13261 tgcgatgagg gatgtcgtat ctcaagttca aggaaaagca attttatgct gtggccttac
    13321 caaccaacgg gaaaccactc tgatttggaa caaaaaaaca ggggaatgcc tcgcgccagc
    13381 tattgtgtgg caagatagac gtacggaaga gttttgttat tccttgtctg aatactcatc
    13441 aatgatacaa gagaaaaccg gattacttcc tgatccctat ttttcagcga gcaaattaaa
    13501 ctggttacta aagaataatg ctcaagctca atcactaatt aaccaggggg atttggcatt
    13561 tgggacaata gacagttttt taatatggcg catgactaaa ggtgccagtc atgctaccga
    13621 tatcaccaac gcatccagaa ctatgctgtt taatattcat gatgaaaaat gggataagca
    13681 gttactcgag cttttttcta tttcagaatc gatattacca gaggtttact ccagtgataa
    13741 tcattttggg gtgatagata aagaatggct gggagtagaa ataccaatta ccggggttgc
    13801 aggcgatcaa caagccgctt taattgggca aggttgtttt aatggcggaa tggttaaagc
    13861 tacttttggt acggggggat ttttattgct caatacaggt gataaaccgg ttttctcaaa
    13921 gcataaattg ttgactacta ttgcctataa aattaaagaa aaaacagctt atggtttgga
    13981 gggtagtatt tatcatgccg gaacaactgt taaatggtta cgcgatgaaa tgaagttgat
    14041 atctcattct gctgaaactg aagtattggc acaaagtctt gatagcaata acggtgtata
    14101 tcttgtttca tcttttactg gtcttggagc acctcattgg ttatcaataa caggaacggc
    14161 tattattggt gtatcccgtt caacgaatag agcgcatttt gccagagccg ccctggaagg
    14221 agtttgttat caaacacgtg atgtattaac ctgtatgaga gatgatagtc aattggattt
    14281 gactttatta agagcggatg gagggatggc agtaaaccaa tggtttctac aatttatggc
    14341 tgatcagtgt cagttgacaa ttcaaaaacc caaggacatt gaaacgactg ccaaaggggc
    14401 agcagtatta gccgcaatag gttctggtct atttgattca gtttcttcag taaataaggt
    14461 atgggacgtt gagaacgagt ttcagccatc cagggaaaga gaggtagttg aaaaagattt
    14521 tcgtggctgg ttagaggcgt tggcaatggt taaagcaagg catgcataga ctagtcttat
    14581 atttaaattg gtgcgattaa attagatcgc agtagctcgt attatagttt aatacaagct
    14641 actgattaac acattggatt ttttatttta cgtcacgaat tttttcacca gtatacaatt
    14701 ggcgaggtct gccaatgcgt gacttggtgg ctaccatttc catccagtga ctcatccatc
    14761 caacagtgcg ggctagagca aatatcactg tgaacatatt ggttggtatt ccaatagcgc
    14821 ttaaagtcaa tcctgagtaa aagtctacat tcgggtagag ttttttctca atgaaatatt
    14881 catcttcaag agcaatacgt tcaagttcca ttgctaattt aaataaaggt gcttcttttg
    14941 ctccaacggc ttctaacaca tcataacagg tcttacgcat tacttttgct cttggatcat
    15001 agcttttata gaccctgtgc ccaaaaccca tcaaacggaa tggatcattt ttatctttgg
    15061 ctcgtttaat gtaatgatcg atgcgactca catcgcctat ttcttttagc atatttaagc
    15121 aggcttcatt cgctccacca tgagctggtc cccataaagc ccctatacca gctgaaatac
    15181 acgcaaaggg gttagctcca gtagagcctg ccagacgtac tgtcgatgta gatgcatttt
    15241 gttcatgatc agcatgtaga ataaaaatag tatccatagc tcttaccaat actggatctg
    15301 gcgtagtatc ttcggatgga acaccaaaca tcatgtgaag aaaattttct gcataagaca
    15361 ttttattttt aggatacatg tagggcatac caatggagta tttataactc attgcggcta
    15421 aagtaggcat tttagcaacc agtctgattg cggaaataaa acggtcttgt gcgttattca
    15481 aatccatgga ttcatgataa aaagcagaca aagcccccac aattccaacc attatagcca
    15541 ttgggtgagc atcacgcctg aacccattca aaaactggta catttgctga tgaaccatag
    15601 tatgattgtt gatcagacta acgaaagctt tcttttcttc tttgttaggt agttctccat
    15661 tgagtaataa ataacttaca tcaaggaagt cctttttttc tgccaattgt tctataggat
    15721 aacccctata caacaaaatg cctttatccc catcaatata ggtgattttt gattcacagg
    15781 aggcagttga taaaaaacct tgatcaaagg taaagacgcc atgtgaacca agctgggtaa
    15841 tatcaatcac atcattacct aaggtgggag tatagattgg taaatcaatc gtctcttgcc
    15901 catcgatgct gagtttcgct gtcttttgag tcattgcttc tcctgattat ttacatgctt
    15961 taagcattca tacttgcgtt agtgcgcgga actatataaa aatgacaaca tacagtcaaa
    16021 ttaaacgtaa ttttggtcat taaaaatgct agtccaaaat ctgacgttag ttatacattc
    16081 aaataattta gatcatttgc aaccgtatac attgattata tattatggtt tacaaatttg
    16141 aggaataata gaaaaaaaaa tgataactcc agctgaatta cctattaatc atcgtggtgc
    16201 ggtatatcat cttgatttaa gaactgaaga aatagctgac ctcatcatta cagttggaga
    16261 ccctgggcga gtgagcttgg ttagtcaaca ttttgataaa attgaagtgc aacgctccca
    16321 ccgagagttt gttacccata cagggtatct cggaactaat agaattacag ttctctcaac
    16381 tggcattggc atgccaaaca tagatattgt gatgaatgaa ctggatgctt tggcaaacat
    16441 cgatttatct acacgaacgg tgaatactaa caaaaagagt ttaacaatta ttcgattagg
    16501 caccacagga ggggtgactg cgaattgtat gcctggagat atagcgatta gtcgatatgc
    16561 tattggtttt gatagtttga ttgattatta tcagtatcag ccgatgggag tgcttgaaac
    16621 aattagggaa gagttatctc agtatctaat gggagagagt ggcccatttt atatttctga
    16681 atcttgcccg caattagctg aacagtttag tgccttaggc catacgggaa ttacagcaac
    16741 ttgttgtggt ttttatgggc cgcaaggccg ccagttaaga ttgcctcttc attaccccaa
    16801 tcttattact cagttaggaa aattttgtac tcagaattat tcagtaatta atctagagat
    16861 ggaaaccgct gctatctatg ctcttggcag tatgcttgga cataaatgct tatccttaag
    16921 tgtggtgatt aataacagga tacatggtag ttttgtaact gatgtcaaat cttgtctgga
    16981 ctccctaatt acaaaatcac ttgcgaatat taaaaatatc cttgagccat atgaagctta
    17041 aggtcattta attaagacgc ccagtgtggt atccttttgc attaaattaa tcttaattta
    17101 ttgtctaaag gatacatcca aatcatggta tacctagcca aagaagtctt accagtcaac
    17161 atagaagacg aattgaagca atcctatttg gattatgcga tgagtgtcat tgtaggccga
    17221 gcgttgcctg atgtacgtga tggtttaaaa ccggtgcata ggcgagttct ttttgcgatg
    17281 agcgagttgg gtaatgattg gaataaacct tataaaaaat ctgctcgtgt agtaggggat
    17341 gtcatcggta aataccatcc tcacggggat acagctgttt atgacaccat tgttcgtatg
    17401 gcccaacctt tttccatgcg ctacctttta atcgatggac aggggaattt tggctctgta
    17461 gatggagatg ctccagctgc catgcgttac actgaagtaa gaatgtccaa agtggcgcat
    17521 gctttactgg ctgatttgga taaggaaact gttgatttta gccctaacta tgatgaaaca
    17581 gaatttgctc cagtggtatt gccatcgaga attcccaatt tactagttaa tggctcttcc
    17641 ggtattgcgg tagggatggc tactaatatt ccaccacata atcttaccga agtaatcaat
    17701 gcatgtattg ccttagtgga tgaacctgac acgagtcttg aagatttaat ggaaattatt
    17761 cctggccctg attttcctac agcagcaatt attaatggtc gtgctggaat tattgaaggt
    17821 tatcgtactg gaaaagggcg ggttgttatc agggcacgaa cagaaattga aacggatgaa
    17881 agttcaggcc gtcagtcaat tattattcag gaattaccct atcaggtgaa taaagcgcgt
    17941 ttgatcgagc gtattgctga attggtaagg gataagaaag tcgagggaat ttccggcttg
    18001 agggatgagt cagacaagca gggaatgaga gtagtcattg aattaaaacg caatgaagta
    18061 gcagatgtgg tattgaataa cctgttcgct catactcaaa tgcaaaatgt attcggaatt
    18121 aatatggttg ctttggtgga tggccaaccg cgtactttga atttgaagca aatactggaa
    18181 tattttataa aacatcgaag agaggttgtt accagacgca caatatttga attgaaaaaa
    18241 gccagaagtc gagctcattt attggagggc ttgggaatcg ccttagctaa tatcgatgaa
    18301 atgatcgcat taattaagca atctcctact cctcaagacg caaaaagtgc tttattaagc
    18361 aaaatatggc aacctggctt agtaaaggcc atgttggaaa aggctggctc aaacgcgtca
    18421 aggcctgatg atttaactga agagtatggt ttgcatgaga acggatataa attatctgaa
    18481 gcgcaggcac aagcaatact tgaattaaga ttacataggc taacagctct tgaacaagat
    18541 aaaataatta atgaatttga agagctattg aatttaatta aagaattgct tgacatattg
    18601 gcttcacctg aaagacttat gcaagttatt cgtgatgaat tgatagagat taaatcccaa
    18661 tttggtgatg agaggcgaac ggaaataaca gcctcacaag aagatttgac tattgaagat
    18721 ttgattaccg aagaagacgt tgtggtcact ttatctcatc aaggttatgt aaaatatcag
    18781 cccattacag cctatcaggc tcagcgccga ggtggcaaag gcaaatcggc aactcatgtc
    18841 aaggatgagg attttgttga gcgtttagtg attgctagta ctcatgatac cttattgtgt
    18901 ttctccaatc atggcaaatt atattggcta aaagcatatc aattgcctca agcaagccgc
    18961 gcttcgagag gaaggcctat aatcaatatt cttcctttgg cagaaggcga agagattaat
    19021 gctatgctcc ctgtccgtga gtataaagac ggcagctatg tgtttatggc aaccaagaag
    19081 ggaacagtta aaaaagtgcc tttaaacgca tttagcaggc cgcgttccaa tggtattatt
    19141 gctgtggacc tggaagaaga tgacagcctc gtcggagtcg atattactga tggtactcgc
    19201 gatattatgt tgtttactga tgcaggcaaa gtaatccgtt ttgatgaaaa taaagtgcga
    19261 cctatggggc gcacggctcg aggggttcgc ggaattcgag tagaaaagga tcaagccgtt
    19321 aaatctcttg tggtagtcga cccaaatgga ggcaccattt tgactgcaac tgaaaacggc
    19381 tacggtaaga gaacccatat cgatgagtat cgagtgtcag gacggggagg acaaggtgtt
    19441 atttccattc aggttactga acgaaatggt aaagtggtcc gttccttgca ggttaccgat
    19501 aatgacgaag ccatgttaat tacagataaa ggaactttgg ttcgttttaa agtcaatgaa
    19561 ttatctgtga taggcagaaa tacacaaggg gttcgcctga ttaatgtcag ttctggtgag
    19621 acagttgttg gaatgcaaaa aattgtagat cttggagaag aattagaaga agcagaagat
    19681 tcatctctta atgccgaaga caacagtgat gaatagcagg gttttcaatt ttggagctgg
    19741 ccctgctatg ctgcccgagg aaatattaaa agaggctcaa gaggaattcc ttaattggcg
    19801 taatactgga atgtccatac ttgaaatagg gcataggaca ccggagatca ttaatctttt
    19861 aagtactgcg gagcaatcgt taagagagct gttaaatatc cctaaaaatt atcatgtatt
    19921 gtttttagga ggagcagctc gagcacaatt tgccatgatc cctatgaatt tattacagcc
    19981 aggggatgag gcggcttatt ttattactgg aatctggtct aagatggctt atcatgaagc
    20041 gaatcttcta aaacaagcat attatcttag caatgaagag aaagagggat ttgtatcaat
    20101 tccggattat caaaagtggg aattaaaaag taatacacct tatgtctatt ataccccaaa
    20161 tgaaacaatt aatggagtca gatttcctta tgttcctaaa acaggagggg ttcctttagt
    20221 cgcggatatg acttcttgct tgctgagcga gccgataaat attaatcaat atggtttgat
    20281 ttttgctggt gcgcagaaga acattgccaa tgcaggttta actatagtta tcattcatga
    20341 ggatttacta aaaaaccaac ctgaaccagc gattcctacc atgttgaatt ataaaaatca
    20401 tgccgaacat cgctccttat atgctacacc accagtattt aattgttatt tggcaagtaa
    20461 aatgttcgag tggataaaaa ctcagggagg tatagaagga gtatttcaaa ggaattgttt
    20521 gaaagccgcg aagttatatc aatatcttga ttcaactgat ttttatctaa ctccagtatc
    20581 taaagaagct cgttctataa tgaatatttg tttttccctc tgctatcctg atttggaaca
    20641 aaaattcctc gatatggcta ataagcgtgg gttaaaggct ttaaaaggtc atcgtttcac
    20701 tggaggattg cgagccagtt tgtacaatgc catgcctatg gctggagttg atgctctcat
    20761 tgagtttatg tctgaatttg caaaagaaaa tggttaataa gcatgttgaa ttttataagt
    20821 aaaccagttg gctgccttaa gggcgaaatc acagtacctg gagataaatc catttcccat
    20881 cgttctatta tttttggcgc gattgctatt ggaacttcag taatcgatgg ttttttagat
    20941 ggtgaagatt gcatagctac ccttaaagca ttccaatcga tgggtgttag gatagagggg
    21001 cctgataagc aacgtgtcat tattcatggg gttgggaaat atggtttaaa acaacctcaa
    21061 aatattattg attgtgtcaa ctcaggtacc agtatgcgct tgctggctgg attgcttgca
    21121 gcacaacaat ttgatagcca gttaacggga gacgaaagtt tacttaaacg cccaatgtta
    21181 agaatcagta gacccttaag tcaaatgggt gcagacgtta ctactcagga tgggaagccg
    21241 cctatagtaa tcaaaggcgg aaaaaaatta aatggaatac attatgttat gccggaagcc
    21301 agtgctcagg taaagtcgtg cctgttgtta gctggtatgt atgctgaagg acaaacaaaa
    21361 ataactgaaa atgcagtcag tcgggatcat accgaaagaa tgttaagaac attttcttat
    21421 ccagttcaga tacaagatgg tgctatcgta attgatcgta atggcgagtg ccatggaact
    21481 cgtctgaata tcccaggaga catttcatct gctgcttttt ttattgttgc agcttctatt
    21541 actccaggct ccgatgtttt aattcgtaat gtaggtatta atcctacacg aactggcatt
    21601 attcatattt taactgaaat gggagcggac ataagagttt tgaatcaacg tgcctatgga
    21661 gaggaaccag ttgctgattt gcatatcaga tattcccaat taaaaggaat cgatattcct
    21721 gcctctatgg tgcctttggc cattgatgaa ttcccggtta tttttattgc agctgcttgc
    21781 gcacaaggga aaacaactct tcatggagct aaagagctgc gcttgaagga aagtgatcgt
    21841 attggcgcga tggtggatgg cttaaaccaa ttgggggttc acgctgaggg ttttgatgat
    21901 ggaatattaa tagagggtgg aagtatacag ggtggtgagg ttaatagtcg aggagatcac
    21961 cgtattgcca tgtcatttgc cattgctggc gctgtcgcca gtgctcctgt aacgattaaa
    22021 aactgcgcca atgttgcaac ttcatttcca tcttttgtta ctaccgctaa catgcttcat
    22081 tttcagatag aggaatacag ctgatggttt ttaataatga ggttcctgtc attacgttgg
    22141 atggtccaag tggaacagga aaggggacaa tttgtcattt ggtagccaag agactgcatt
    22201 ggaatatgct tgacagtggt gctatttatc gtgttctagc ttatgctgct cggaaaaata
    22261 gcatcgaacc gaatgatatt aaaaaattga ctgctttggc ccgttccttg aatttgcgtt
    22321 ttgaatcatc tgcggataat gaatcaaaag taatacttga tgatgaaaat gtcagccagc
    22381 aaatccgcag tgaacaatgt ggtcaggatg cctcgcaaat tgcagtgatt cctgaagtaa
    22441 gagcagcact gcttgaacgc cagagaaact ttgcccaatt tcctggattg gtgaccgatg
    22501 gacgcgatat gggtacggtg gtttttccaa atgctgtttt gaaggtttat ttatatgcaa
    22561 gtgcagaaga aagagcaaat agacgatatt tacagttgca agataatgga attaatgtta
    22621 gcctcgccca ggttgtagaa gaactggcta aacgggatgc aagggataca gctcgtacgc
    22681 atgcgccatt aaaaccagct gaagatgctg tcttgatcga tacaaccgga ctaacaattg
    22741 tacaagtgtt caatattgta ttaaaattaa ttgatgaacg tttgaataat ttgtagtttt
    22801 gaattggggg gaaggtgagt ggaattcatt cttccatttt tttactaaag agtttattaa
    22861 catgtctgaa agtttcaaag aattatttga gcaaagtatt gccggagctc aattttatcc
    22921 tggtgccatt attactgcta aagtcatcga tattgatgat gattttgtta ctctcaatgc
    22981 tggactaaag tcagaaggta ttgttgccgt agaagaattc tacgataaaa atggcgaatt
    23041 ggaagttaaa gtaggcgata ctgtcgaagt ggccctggat tcagtagaag atggtcacgg
    23101 cgaaacgctc ctttcaagag aaaaagcaaa acgccaggaa gcatggcgca agttatcgaa
    23161 atgccacgaa aacaatgaaa cagtcactgg tttgatttct ggaaaagtca aaggtggttt
    23221 tactgtggaa attggctcaa tcagagcatt tttaccgggt tcccttgttg atgttaggcc
    23281 tgtaagagat ccatcttacc tcgaaggtaa agaacttgaa tttaaagtca tcaagatgga
    23341 tttgaaacgc aataatattg ttgtttctcg acgagctgta gttgaagaag aaagtagcgc
    23401 tgacagacaa gcactactgg aatctttaca tgatggtcaa gagcttcacg gtattgttaa
    23461 aaaccttacc gactatggtg ctttcattga tcttggcggc atagatggtt tgttacatat
    23521 cactgacatt tcctggaagc gagtcaagca tccaagtgaa gtattatcag taggtcagga
    23581 tgttaaagtc aaagtactaa gctttgatag tgaaagaaac cgcgtttctt tgggtatgaa
    23641 gcagttagga aatgatcctt gggttgattt ggttgaacgt tacccaatag gcaagagatt
    23701 gcaaggtaaa gtaactaaca tcaccgatta tggttgcttt gtcgaaattg aggaaggtgt
    23761 agaaggttta gtccacatgt cagaaatgga ttggactaat aagaatgttc acccaagcaa
    23821 agtagtgtct ctgggagatg tagtcgacgt tatggttctt gaaattgatg aagaacgacg
    23881 acgtatttct ttaggtatga aacagtgtgt gggcaatcca tggcagcaat tcgcatctac
    23941 tcataataaa ggcgaaaaag taaaaggcaa gatccgttct attactgact ttggaatctt
    24001 tataggtctt gatggtgata ttgatggact agttcattta tctgatattt cctggactgt
    24061 tcctggtgaa gaagcggtaa aacaatttaa aaagggccaa gagttagaag ctgttatttt
    24121 agcaatcgat ccagagagag agcgtatttc tttagggttg aaacaattgg aaggcgacag
    24181 ttttgccagc tttgctgaaa cttacacgaa aggatctatt gttaaaggta cagttacagc
    24241 tgtagaacct aaaactgtaa cagttgcgtt agcagaagat gtttctggga caattcgtgt
    24301 gagtgagtta tccgatgaac gtgttgatga tgcgaccact atcgtaaaag ttggtgatga
    24361 agttgaagca aaaatcacta atattgatag aaaaaatcgc accatttcac tttctgtcaa
    24421 ggcaaaagat gcccaggatg aagcggatgc aattaagaag tactccagaa cagaggcagc
    24481 atcaactact ctgggtgatt tattgaaaga aaaaatggct agcaaagaag gtgaataacc
    24541 tttttaagtc aaaactaaaa aagcgtagaa ctatctacgc ttttttttta ttttgagaaa
    24601 aagattatgg tatgatggac gttttagctt aaggattaaa ggaaatattc aatgcgcata
    24661 ttaatgctgg taacttattt gctgcttatt attattggtg ttagttttgc ggctctaaat
    24721 gcctcgtcgg ttcaagttaa tttctatttt aaaacggtg
//
LOCUS       DAXLDT010000038        24312 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXLDT010000038 DAXLDT010000000
VERSION     DAXLDT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24312
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.038"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            91..1860
                     /locus_tag="ACF2SP_001182"
     CDS             91..1860
                     /locus_tag="ACF2SP_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFD2358976.1"
                     /translation="MNQEISNFSTFNFSSALNKALEDMKFITPSPIQAQTIPLILEGR
                     DAIALAQTGTGKTAAFALPILQNLSPEISTTQALILAPTRELAIQVAEQFELLSKYQR
                     NVTIAVLCGGQEYGRQLKQLRSGAQVVVGTPGRILDHIDKGTLLLNNLKTFILDEADE
                     MLRMGFIEDVETILEKLPEKKQMALFSATMPYRIRQIANTYLNDPASIEIRMETATVK
                     SIEQRFLFASVHQKPDALIRVLEVEDYQGVIVFVRTKSSTEEVAELLQQHGLRAMAIH
                     GDITQSLRERIIAQFKQGAIDILVATDVAARGLDVERVTHVINYDMPHDNETYVHRIG
                     RTGRAGRSGVTILFVTPKESRLISSIERHTRQRIEKVQVPNDHMIQMARQQRFMSNIT
                     TRLNHEHVYSYKRIVEEYIKEHDVSAVDVAAALALLLHKDMPWRKELNMPKAASAFKE
                     ERARVGEKGLDLRGRFKEDRKSSFADRKGARGSKKDFAKDFSNERKKFNDGRKAVNQE
                     SVAQDLFRIDVGKIHGVKPGNIVGAIANEAGLQSRYITGLKIHEDHSTVRLPKGMPKE
                     VMKDLTKAWVCGRQLNLTLIGSA"
     gene            2211..2429
                     /locus_tag="ACF2SP_001183"
     CDS             2211..2429
                     /locus_tag="ACF2SP_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358977.1"
                     /translation="MKKKHEPGFFGRETKNIKDIESNRYDPDCPIRRADKSRGFSQKA
                     SGQFQQRIKDEVKEALDDIRNESAYKFN"
     gene            complement(2586..3101)
                     /gene="mavE"
                     /locus_tag="ACF2SP_001184"
     CDS             complement(2586..3101)
                     /gene="mavE"
                     /locus_tag="ACF2SP_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavE"
                     /protein_id="HFD2358978.1"
                     /translation="MTRFERNFLINSLMFLETILSVDKKLDDAIHHFTQGQYENPRYQ
                     INSRITNADDWSKEDKLKFTSAIAEAIALVSEKYENPTSETTEQIQSARNILLDDYVP
                     LLTANTDPENRLKSVRENSSQIRKELIAKLKDEVPYKSQFENPYVLFPFVAATVAVAA
                     TAASVLFGNKP"
     gene            3371..4300
                     /gene="plaA"
                     /locus_tag="ACF2SP_001185"
     CDS             3371..4300
                     /gene="plaA"
                     /locus_tag="ACF2SP_001185"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lysophospholipase PlaA"
                     /protein_id="HFD2358979.1"
                     /translation="MKLLASLCAFLLSGVVSATPLNNIVVFGDSLSDNGNLYEYMKHQ
                     LPQSPPYFEGRFSNGPVWIERLAASYFPNDPNSHLLDYAFGGAGVSVDEEDDEVFFTL
                     RREVNSYLLAHQDKASPDSLFVIWIGANNYLGMPVEVEETLKNVNRGITDSIQRLVDK
                     GAKHILVLNLPDLGRTPAALEFGSVEEMTYFSTQHNNALSNTVDYFKKTYPEVEWLFF
                     DTGSHFDHVIEHAAEYGFTNITGTCSFSIVDEITKNSVLKMVASVKPELTESACDGYL
                     FFDLVHPTALAHKIMAEKARLMLDEAGVEFAEN"
     gene            4492..5265
                     /locus_tag="ACF2SP_001186"
     CDS             4492..5265
                     /locus_tag="ACF2SP_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HFD2358980.1"
                     /translation="MNQIKWIVLLFCFVVSNVFADKLDITVDQKKIILPYWLSRSVSY
                     GAVIIVSGGETAQWSLLLEQFAKGLSRNGWSVALLNCTKNNSIPWINQLPEVISTLRQ
                     NNNKRIVLVHYGDQLNLVLEYFSKPQSKMINGLVMLSAYDLNRNLRKVPRLRFPLFDI
                     VGQFDYDMVRQQRKSREEKFKENSYLAIDIPGATHDYQYSQQLLLAFVHGWMAKLPEF
                     EPQPPPILVSYLEPVYSTASSIVSTNKLDYQPLVSQINS"
     gene            complement(5232..6122)
                     /gene="djlA"
                     /locus_tag="ACF2SP_001187"
     CDS             complement(5232..6122)
                     /gene="djlA"
                     /locus_tag="ACF2SP_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFD2358981.1"
                     /translation="MNLRDFFVITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRG
                     LVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVAKSDGRVSEQEISMAKSIMNEMK
                     LSKGQKDLAKRLFNEGKQPDFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
                     SQKIHALDNIFTHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQGYKHTSSSQDQQ
                     GYKPQSPPNTLAHAFALLEVSPNASKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLA
                     NDKTHQIMKAYELICETKGW"
     gene            6277..7536
                     /gene="waaA"
                     /locus_tag="ACF2SP_001188"
     CDS             6277..7536
                     /gene="waaA"
                     /locus_tag="ACF2SP_001188"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFD2358982.1"
                     /translation="MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQE
                     NAPVDVWLHAVSLGEVIAATPLIDAMLNKRWSVLVTTMTPTGSERVKSRFGHKVAHQY
                     LPYDLPWVLKRFFKRTRPRVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGY
                     LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQY
                     RELKSHWGEDRPTIIAASTHDDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQ
                     LSVQAGFNTGCRSNLNTISRENEVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVL
                     EPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHVNELVDAIIKLYQDRESQNTMV
                     ANASSVLESNKGSVVRYLQKIESALGY"
     gene            complement(7541..8374)
                     /locus_tag="ACF2SP_001189"
     CDS             complement(7541..8374)
                     /locus_tag="ACF2SP_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2358983.1"
                     /translation="MFFMYFTNYGYRLNQIKKGSDQHWLFLPGGPGLGSEYLASFCDK
                     LTLQGTVSLVDFPKDGNNSEGQLDFVSWQKGLISLLKSLNKPILVTHSFSGMFTLNFP
                     EIENHLSGLVLMNTTTTNSFFQHVNEMRQKHNLPDLVPAASQYHLYPSNETYKKFWDT
                     YKHYCFTQEELPIGEQMMELFAYNNESYHYAIEHFYNDFHYKWHPQSIPTMTIASELD
                     YICPPAIFIQDKNFQCQNVINKVIYKAGHCPWILHLEQVQKCFDEFFGMIQEINIERT
                     L"
     gene            complement(8444..8851)
                     /gene="dksA"
                     /locus_tag="ACF2SP_001190"
     CDS             complement(8444..8851)
                     /gene="dksA"
                     /locus_tag="ACF2SP_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFD2358984.1"
                     /translation="MGITPYQETEGEEYMNEKQLAHIEKILLAWRQSLMEEVDRTVSH
                     MKDEAANFPDPSDRASQEEEFSIELRTRDRERKLIKKIEDALERLRNEDFGYCEACGI
                     EIGLKRLEARPTATLCIDCKTLSEIKERQNQGA"
     gene            complement(9019..9882)
                     /gene="prmC"
                     /locus_tag="ACF2SP_001191"
     CDS             complement(9019..9882)
                     /gene="prmC"
                     /locus_tag="ACF2SP_001191"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFD2358985.1"
                     /translation="MIDIRSLLEQALQQFKVKNQETKLEAELLLCHVLNKNRAYLFAH
                     PDALVSPEQIETYLQMIKQRAEGLPIAYITGQREFWSLSLKVTPNVLIPRHETERLVE
                     LALELIPDKENVSVLDLGTGSGAIALALAKERPLWHIDACDFSKEALELARYNAKTLG
                     LNNINFYHSCWFNNLPLKQYHAIVSNPPYIAENDPHLKQGDVRFEPTSALVSSQDGLA
                     DLQYIIQHSYEYLLPDGLLLVEHGFEQKNEISAILNQLGYKNIHCWQDLQGHDRVSGG
                     QRPSNRNNKPL"
     gene            complement(9875..10963)
                     /gene="prfA"
                     /locus_tag="ACF2SP_001192"
     CDS             complement(9875..10963)
                     /gene="prfA"
                     /locus_tag="ACF2SP_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFD2358986.1"
                     /translation="MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQ
                     LEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMAEEEIDTVKKQIEELDEQLQWHL
                     IPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
                     YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQ
                     INPDDLRIDTYRSSGAGGQHVNKTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLL
                     KTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDI
                     MEGNLSLVIDPLKREYHAELLADLGRHD"
     gene            complement(10947..12221)
                     /gene="hemA"
                     /locus_tag="ACF2SP_001193"
     CDS             complement(10947..12221)
                     /gene="hemA"
                     /locus_tag="ACF2SP_001193"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFD2358987.1"
                     /translation="MVFVACGLNHKTAPIHVREKVALQPAMQDSLLSSLLDLPEVNEA
                     AILSTCNRTEIYCDTNTPEVLGNWLAHEHQLSEELLSQFLYIHQGKEGIKHTLRVASG
                     LDSMMIGEPQILGQMKQAYQHACRLGTVKTQLRPVFEYIFRASKRIRTRSGIGANPVS
                     IAYAAVQLIGQLFKNYHSLSVFLIGSGETASLVAKYLHQHGVHRFLIASRTLENAQKL
                     AETFGGKTLSIGDIPQYLPLADVVISATACPLPFINKSLVEHALEQRNHAPMFLLDLA
                     VPRDIEGNVNELEQVHLYNVDDLQSMIEKGMDERRNAALQAEQLIESELDNYIRWHRS
                     LRAKDVICDYRNQMHTLAQQELQRALKKISAGQNQQDVLNEFSMRLVNKLTHNPTIGL
                     RQIAWDNREDLLDLARYLFDTTANQSLYEEIS"
     gene            complement(12345..12500)
                     /locus_tag="ACF2SP_001194"
     CDS             complement(12345..12500)
                     /locus_tag="ACF2SP_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358988.1"
                     /translation="MAIHVGGKTQQDFRKSLFSLLDMSPFEDRESKLNPLPANFPKWD
                     NLEEEGG"
     gene            12539..12817
                     /locus_tag="ACF2SP_001195"
     CDS             12539..12817
                     /locus_tag="ACF2SP_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2358989.1"
                     /translation="MDIILQGQHNAIETVESLERVLKLFKERYQVDGFREIHLTVTLV
                     DEQGDEVELVDSDTNQPYRIFEVYRKGYELAGRKGIPMLQLVVDNTHK"
     gene            12874..13947
                     /locus_tag="ACF2SP_001196"
     CDS             12874..13947
                     /locus_tag="ACF2SP_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFD2358990.1"
                     /translation="MIMALVAIILTLILVIGIHELGHALAARLFQVKISKISIGFGKP
                     LIQWQTQSGCNWIWAMWPLGGYVQLLNSRISPVKPQENAYCFDKKPIWIRVLILLSGA
                     IANLITAWIALVLVFYIGISYKQPQIQSVKLDSLAAKAGIQAGDQWVSVENYPTDSWQ
                     GVGMQLVIHWGQKNVHIVLRQANQQLKQLSLDLSQIEFTSKDGSLLESLGIKPDLSVV
                     SSLTRYPSLLASMQKAFAEIIHTMYFFIMILKQLFLGVIPFSILLGPLAIFSVSVASL
                     TQGVIVFLLFIATLSLAVALVNLFPIPGLDGGSILYSVIEKIRGKPVSVAVEVLLHRL
                     MIILFCVLLVHLLMNDLNRYLHH"
     gene            complement(13976..14680)
                     /locus_tag="ACF2SP_001197"
     CDS             complement(13976..14680)
                     /locus_tag="ACF2SP_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFD2358991.1"
                     /translation="MLQKFLSITQNLRLPSICTLCNQFHKSQLAVCSNCMEFMKQLGS
                     CCQYCAYPLSDDTYLVCGQCVRKRPSFDSAYIAYRFEEPLRSLIHQFKYHNGLYLASF
                     LKQLLLNALPKSALKPDCLIPVPMHPKRLKRRGFNQAAVLTRLLARQLNIPYDLYYCQ
                     KIINTASQANLDGEQRRKNLRHAFYVPPVTYEHVMIVDDLLTTGSTANEIAHTLKNAG
                     VKRVDICCCARAVTKN"
     gene            14724..15578
                     /gene="bioC"
                     /locus_tag="ACF2SP_001198"
     CDS             14724..15578
                     /gene="bioC"
                     /locus_tag="ACF2SP_001198"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFD2358992.1"
                     /translation="MTVNNEISKAFSKHADDYERVAKVQKEIGSRLFERLQYLKIAPR
                     RILDLGCGPGFFSKELALLYPKAQIVGMDLSFAMLEQARKKQGWRRKWPLVSADMQKM
                     PFATGAFDLVFANQVIHWSSSLGTVFRELNRVMNVNGCLMFTTLGPDTFKELQTAWSA
                     ANQYAHVNEFVDMHDIGDCLIAEHFMDPVIDMELLSIHYETLPQLLLALKTQGVRNIN
                     PKRNHGLTGKSAWKQFEAQYATMRTTTGKYPLTYEVVYGQAWKGAQRKMDQGIETWIP
                     VSRVVKKS"
     gene            complement(15622..16518)
                     /locus_tag="ACF2SP_001199"
     CDS             complement(15622..16518)
                     /locus_tag="ACF2SP_001199"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFD2358993.1"
                     /translation="MNKQVIDKYTQRHELYLEQLLNEIIIPAPKIRSALHYALFSGGK
                     RIRPILVYLAGDLIDVDQGVLDIIAAALELTHCYSLIHDDLPAMDNDDLRRGKPSCHK
                     AFDEATAILVGDGMQALAIEVVLTRLSPLLPAAQVVAITQVLVNASGISGMVSGQSLD
                     LSELAKLSVTEEQLREIHLLKTGKLILACFEMVLAAQNEVSEQIKSALKTYGKHIGLV
                     FQMQDDYLDLYAPTQILGKGRSSDQANQKTTFATLFNKQQLEEEIAVHYQIAMDSLRL
                     FGSKAAALIELTKQLQNRSNLS"
     gene            complement(16499..16729)
                     /locus_tag="ACF2SP_001200"
     CDS             complement(16499..16729)
                     /locus_tag="ACF2SP_001200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFD2358994.1"
                     /translation="MSKGIHFEQSITELEEIVRQLEKGELTLEESLKQFEKGISLARR
                     CQNALNQAEQKIETLTGTDSNIELDSDEQTSD"
     gene            complement(16923..17306)
                     /gene="lem22"
                     /locus_tag="ACF2SP_001201"
     CDS             complement(16923..17306)
                     /gene="lem22"
                     /locus_tag="ACF2SP_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem22"
                     /protein_id="HFD2358995.1"
                     /translation="MPAFKKGQNPVTELTRLKEYMEDQIAKAKESSSLTAQLKFLENA
                     HTEHFVKMGSLTTIYKGGSEVVDRLKIEIRSLYEEMLALKDKCRDQIQQHETSIKNSP
                     TFFTTKNNKTKDEEFDLDFDKAFGL"
     gene            complement(17571..18449)
                     /locus_tag="ACF2SP_001202"
     CDS             complement(17571..18449)
                     /locus_tag="ACF2SP_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2327 family Dot/Icm T4SS effector"
                     /protein_id="HFD2358996.1"
                     /translation="MAKFTVGQDPVKGLTELKAYMEEQISKIKKATSEQDIDQLLIDT
                     LNEYDDKMGSLSKVYKGGNEQVEQLKIDVRKLYEPLRDQFSHKHPLTLVSEVQTKLEE
                     QRKQAEVEKLKREEQLKLEKQVEEEKLKLAKQSEEEKPKLENQQEETITQALQKVDGI
                     IQELTLKIDRVDQHQYKKAHDTANTLLQSLIAARDEYERDLRANEFSPELAGRKFKLA
                     CQDAVKIAKPVLEKDLGWGDYLKNLLKCLGNAVITVFTFGCQQGFFAYARPDSAKAVE
                     KAEEDLGLRQAASSPK"
     gene            18684..20036
                     /gene="radA"
                     /locus_tag="ACF2SP_001203"
     CDS             18684..20036
                     /gene="radA"
                     /locus_tag="ACF2SP_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFD2358997.1"
                     /translation="MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIVPENKTS
                     RSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKS
                     TLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
                     KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEG
                     TLAGPRVLEHMVDSVLYFEGQNDSRFRVIRAIKNRFGAVNELGVFAMNDKGLKEVANP
                     SAIFLSRQPEPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAIL
                     LAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDSETIIFGE
                     VGLAGEIRPVQSGQERIKEAIKHGFKRAIVPFANAPKQQLDSMTVEPVKHLRDVLEKM
                     "
     gene            20033..20869
                     /locus_tag="ACF2SP_001204"
     CDS             20033..20869
                     /locus_tag="ACF2SP_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HFD2358998.1"
                     /translation="MSYQKPITPEYKMNEDTIRDFNRDGAVCLRQFLNAEEIAILKEG
                     IEFNLSSLSARAKIASRHDDPGYFVEDFCTWQNNPSYQRIIFGTNLGVAAAQIMQSQS
                     VRLYHDHLLVKEPGTRQVTPWHQDQPYYNIEGKQNCSFWIPVDPVSRSSTLEFIAGSH
                     LGPWLMPRTFLDNQAKWFPEGSLQELPPIEEARARYPIIGWEIEPGDVVCFHMLTLHM
                     ARGVEGRHRRRVFSVRFLGDDITHAPRAWPTSPDFPGLTAEIPAGASMDHPLFPCLPL
                     KI"
     gene            21156..23084
                     /locus_tag="ACF2SP_001205"
     CDS             21156..23084
                     /locus_tag="ACF2SP_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-gulono-gamma-lactone oxidase"
                     /protein_id="HFD2358999.1"
                     /translation="MALPTELKSKFIQILNQNKISHSVGWSNYMESVNNPTAIVVEVK
                     NEKEVQTVMKAIKELNSDREPKDKVTVRATAGWTDKQGKSCCFFPWSEAQESQYNEGF
                     SFSQVVGGKESHDTAGTDVIIRFAKKFHHAKVIGSLKNPPVFNPDNPIHRLPVTLVEV
                     SAGMQVADFADFLREHQLSLSTVSMISWVTPVGLAGTAGHGTGRDEPAFSGLIESVTV
                     CDMDGNLREINSDHPDFKDLTGGHSGLLGIVVSMKLRAVKAFNLCETIELFPNTREMT
                     GRLSDILKNNQYISIMGMPSYGCPESDKLVSKWQIRQWNYTTDKPGKKEKAPYDPDVR
                     SFAQELQVRIGASVMDFLLDSGLKHLLPSFMLLSAAVVTGTRGTSPKVDYENHITHPQ
                     VAFPKEMRDVSYLIPVKDDEAGQKLELILQKMEAMLNEAGSKGEFPVTYAIYVRYLKG
                     TNGGLSTSFTSSDNERILAIDVVTHPEAPGITNFEKGFMSFLKEMNIIPRNHLGKNFP
                     AGVVHYDQFLGAERVSKYKQALERFYCSPGKNDGAERLSMSPFYTPYLQGMLSASAPT
                     KQLVDENKYEQRIERLHEEHSDLECAEFLSKLYAEVSRMSTSSEEALTAKKGFLEACK
                     AELEHRNSRLEAPVPLLS"
     gene            23174..24295
                     /locus_tag="ACF2SP_001206"
     CDS             23174..24295
                     /locus_tag="ACF2SP_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFD2359000.1"
                     /translation="MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKS
                     NQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGE
                     IPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
                     PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYA
                     ETGHLCISTLHANNANQALDRVINFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQR
                     VAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQALFNLYKEGKITKENA
                     IKYADSKNNVGLQIRLGEEKNFNIQDENLQIQPDDIDKI"
BASE COUNT         7028 a         4389 c         5200 g         7695 t
ORIGIN      
        1 gcatcatctt gttaattttg ttattataat gcacattttt tatattagtt agccttttca
       61 gaaagacgct ttattatatt caggaacagt atgaatcaag aaatatcaaa cttctctact
      121 tttaattttt ctagtgcttt aaataaagcg ttagaagata tgaagtttat tacgccatcg
      181 cctatccagg ctcaaaccat tcctttgata ttggaaggtc gtgatgccat tgctcttgcg
      241 caaactggta caggtaaaac agcagctttt gcattaccaa ttttgcaaaa tctgtctcct
      301 gagatatcta ccactcaggc tttgatttta gcccctactc gtgaattggc aatccaggtt
      361 gcagagcagt ttgaattatt aagcaagtac caacgcaatg tgacgattgc tgttctttgc
      421 ggtgggcaag aatatggtcg tcaattaaaa caattgcgta gtggtgcgca agtggttgtt
      481 ggaacgccag gacgtattct ggaccatatc gataaaggca ctttgctctt aaataatctg
      541 aagacattta ttcttgacga agcagatgaa atgttgcgta tgggtttcat cgaagatgta
      601 gaaaccattt tagaaaaact gcctgaaaaa aagcaaatgg cattgttttc agcaaccatg
      661 ccctatcgaa ttcgtcaaat agccaatact tatttgaatg accctgcctc aattgaaatc
      721 cgtatggaaa ctgcgacggt caagtctatt gagcaacggt ttttatttgc ttccgttcat
      781 caaaaaccgg atgcgttaat ccgggtcttg gaagtggagg attatcaagg ggttatcgtt
      841 tttgtacgca ctaaaagcag tactgaggaa gtagcagaac tattgcaaca acatggatta
      901 agagcaatgg caattcatgg tgatattact cagtctttgc gtgagcggat tattgcccaa
      961 tttaaacaag gagcgattga tattctggtg gcgacggatg tcgctgcaag aggtttggat
     1021 gtcgagcgag ttactcatgt gattaattat gatatgccac atgacaatga aacttatgtt
     1081 cacagaattg gcagaacagg gagggctggc cgttctggtg ttacgatttt atttgtaact
     1141 cccaaggagt cgcgtttaat ttccagcatt gaacgccata ccaggcaacg aattgagaaa
     1201 gttcaagttc ctaatgacca tatgatacag atggctcgtc aacaaagatt catgtctaac
     1261 attactactc gactgaatca tgagcatgtg tattcctaca aaaggattgt ggaagaatac
     1321 attaaagagc atgacgtatc agcagtagat gttgcggctg ctcttgcttt attgctgcat
     1381 aaagatatgc cttggagaaa agaattaaat atgcctaaag cagcttcagc attcaaagaa
     1441 gaaagagctc gggtaggaga aaaaggattg gatttacgtg gtcgatttaa ggaagataga
     1501 aaaagctctt ttgctgatcg gaaaggtgcg cgaggaagca agaaagattt tgccaaagat
     1561 ttttctaatg aaaggaaaaa atttaatgat ggcaggaagg ccgttaatca agaatcagta
     1621 gcccaggatt tatttcgcat tgatgtaggc aaaattcatg gagtcaaacc tggcaacata
     1681 gtaggggcta ttgctaatga ggctggcttg caaagccgat atattactgg gcttaaaatt
     1741 catgaagatc actccactgt tcgtttacca aaaggtatgc ctaaagaagt gatgaaggac
     1801 ttaaccaagg cttgggtttg cggccgccaa ctcaatttaa cattaattgg tagtgcctga
     1861 atagctcagc agtaaagcgc tgttagcgct accaattggc ctttaattat tttgcaaaac
     1921 gatttcgcct gttcagggcg agatcataac attaagtaca ataaatagcg caaacaatgg
     1981 tgcacatttt attatcctcg cacaggcagg aagctatctc ttctctatag caaacggagg
     2041 ggcaagattt gaaatagatt cccactcgcg caggaatgat acgcatgacg tattatttaa
     2101 tcaaagtatg attttaccct gaagacctac cttttttctg gcttgttttt ctaatttaat
     2161 acctctaata tggattggtg ttctgaaatt taacactgag gggccaaatt atgaaaaaaa
     2221 aacatgaacc cggttttttc ggtagagaaa ccaagaatat caaagacatt gaaagcaata
     2281 gatacgatcc tgattgccca attcggagag ctgataagtc tcgtggattc agccaaaaag
     2341 caagtggtca gtttcagcaa cgaataaaag acgaagtaaa agaagcgtta gacgacattc
     2401 gtaatgaatc tgcctataaa tttaattaac cattatttta tctctggatg taagagatca
     2461 gctcctgctt gaaacgaccg tatttggtcc atcttgaacg gaaataaccc ttggagcact
     2521 gatctgtata tttctgagtt taatgaagcc tgacaaaagg cttcattagt aataaaacac
     2581 tacctttatg gtttgttgcc aaacaacact gacgcagcag tagctgctac ggctacagtg
     2641 gcagcaacga atgggaaaag aacatagggg ttttcaaact gacttttata aggtacttcg
     2701 tctttaagtt tggcaattaa ttcttttctt atctgactac tattttctcg cacagatttt
     2761 aatctattct cagggtcggt gttcgctgta agtaatggaa catagtcatc caataatata
     2821 tttcgggcac tttgtatttg ctctgtagtc tcactggttg gattttcata tttctccgaa
     2881 acaagagcta tggcttctgc gattgcagaa gtgaatttta gcttatcttc tttggaccag
     2941 tcatcagcat ttgttatacg tgaattaatt tgatagcggg ggttttcata ttggccttga
     3001 gtaaaatggt gaattgcatc atccagcttc ttgtccacag agagtatggt ttcaagaaac
     3061 atcaagctat taatcaggaa atttctttca aatctagtca ttattatggt tctcataaag
     3121 aaataggggc tgacgacatt tctgctgaat cgatcgcaag atattgattt tctgtaccct
     3181 gttgcaaaag aaagcattat gaatcgagtc agggaagtgt ttacaggatt tactataatt
     3241 aggtttctgt tttagatcaa caaaaaattt atatacagta atgtgttaaa tgccgtggaa
     3301 aaattgataa atcattatat aattgaatga acctaaagat ataaaatcaa tctattattt
     3361 aaggggtttc atgaaactat tagcatcttt gtgtgctttt ttattgtcgg gagttgttag
     3421 cgcgacacca cttaataaca tagttgtatt tggtgatagt ttgtcggata acggtaactt
     3481 atatgaatac atgaaacacc aacttcctca atctccccct tattttgaag gtcgtttttc
     3541 caatggccca gtatggatag aacgtctggc agcttcatac ttcccaaatg atcccaattc
     3601 tcatttgctg gattatgctt ttggtggggc aggagtctct gtcgatgaag aagatgatga
     3661 agtatttttt accttaagaa gagaagtgaa ttcttatttg ttagcacatc aggataaagc
     3721 aagtcccgac agtctttttg ttatttggat aggggcaaat aattacctcg gtatgcctgt
     3781 tgaagtagag gaaacgctta aaaatgttaa ccgagggatt acggatagca tacaacgttt
     3841 ggtagacaag ggcgcgaagc atattttagt tctcaattta cctgatttgg gaagaactcc
     3901 tgccgccctg gagtttggct ctgttgaaga gatgacctat ttctcaacac aacataataa
     3961 tgcattatcc aatacagttg attattttaa aaaaacttat cctgaagttg agtggttgtt
     4021 ttttgacacg ggtagtcatt ttgaccatgt gatagaacat gctgcagagt atggatttac
     4081 aaatataaca ggcacctgct ccttttcgat agtagatgag attactaaaa actccgtttt
     4141 gaagatggtt gcgtctgtga agccagaatt gacagaaagc gcatgtgatg gttatttgtt
     4201 ttttgatttg gttcatccga cagcgttggc acataaaata atggcagaaa aggcgcgttt
     4261 gatgttggat gaagctggtg tggagttcgc cgagaattaa gagtttttta tggcgatagc
     4321 aaatgaaact tgcatcgtca ttcaaatgaa tccgcaatga taagagcggg gattttatac
     4381 tgagcagaac ccaatgagag agtggtgtat ttgaacttca caaatattct ctcattctaa
     4441 ttggcactag ctagtgcgtc ttgtccatca taggcaattt tatagggaaa tatgaatcaa
     4501 attaaatgga tagtgctact attttgtttt gttgtttcca atgttttcgc ggataagctt
     4561 gacataaccg tcgatcaaaa aaaaatcata ttgccttatt ggttatcaag atctgtttcc
     4621 tatggggcgg ttatcattgt tagtggcgga gagacagcgc aatggtcctt attattggaa
     4681 cagtttgcca agggattgtc tcgaaatggt tggtcagtcg cactattgaa ttgtactaaa
     4741 aataactcga taccctggat taaccaatta ccggaagtta tcagtacttt aaggcagaat
     4801 aataataaaa gaatcgtttt agtccattat ggtgatcaac ttaatctggt attagagtat
     4861 tttagcaagc cccaatccaa gatgatcaat gggctagtga tgttgtctgc ctatgattta
     4921 aatagaaatt tgcgaaaagt acctcggttg cgttttccac tgtttgatat tgtggggcag
     4981 tttgattatg acatggtaag gcagcagcga aagagcaggg aagaaaaatt taaagagaat
     5041 agctatttag ccattgatat acctggtgct actcacgact accaatacag tcaacaatta
     5101 ttattggcgt ttgttcatgg ttggatggca aagcttccag aatttgagcc tcagccaccg
     5161 ccgatattgg tttcttatct ggaaccggtt tattctacag caagttcaat cgtatcgacc
     5221 aacaaattgg actaccagcc cttggtttca cagatcaatt cataggcttt cataatttga
     5281 tgagttttat cgttagccaa tttaatcatt tcttcgggta aaccttgagc aattaattta
     5341 tctggatggt tacggctcaa taggcgtctg taagctcttc taacctcctg tttgcttgca
     5401 ttcggactta cctcaagcaa tgcaaaagcg tgcgctaaag tattaggggg tgattgaggt
     5461 ttgtaaccct gttgatcttg agaagaggag gtatgcttgt acccctgttg attatgatag
     5521 tgttgtttgg attgttcttg ctggaaataa gagccaaaat cctcataaaa acgatactgc
     5581 ttgtgtaagg gagcaaatcc cagatgcgta aaaatgttat ctagcgcatg aattttttgg
     5641 ctacttaatc catctacttg agctgcccta tactgaatat ctacaaaaag ctttaacaaa
     5701 tcacggttat ccttgcagat tctttgtaat tggataagag caaggctgac attgaaatca
     5761 ggttgtttcc cttcattaaa taatcgtttt gctaaatctt tttgtccctt gctcaatttc
     5821 atttcattca tgatggattt agccatgcta atctcttgtt cagaaacacg tccatccgat
     5881 ttcgcaacat gccccattat ggagaaagtg gcttcaaaga aagctttttg aacgattctt
     5941 tgtttttctg catggtacag ccaatgggga ttagagtaat aactgacaag gccccgatca
     6001 aaaaagtttc caacgagaat gccaaacaaa gcgcctactg gtccagcagt taagtagcca
     6061 aagaaggcac caagtatttt tccccaccag gttgttatta caaagaaatc acgtaagttc
     6121 attatcagaa attacctata aagatatgtg ttaaatttaa cttcaagttc taataatata
     6181 gattgaggac aacatagtct ctaattgaat tgtaacatat cgcacaagga actgttttag
     6241 gtatatactt tacctttttg tgtttggtgc ctgtttatgc gttttgtgta ttccttttta
     6301 atgtacttac tgactcctta cttgcttttt cgcctgtggc ggaaaggtag aaagttgcca
     6361 gcttacaggc aaaggatagg agagcgattt tgtctgggaa tacaagaaaa tgcaccagtc
     6421 gatgtatggc ttcatgcggt ttctctggga gaagtgatcg cggcaacacc cttaattgat
     6481 gcaatgctca acaaacgttg gtcagtgtta gtcacaacaa tgacccctac aggttctgaa
     6541 cgagtaaaat ctcgatttgg tcataaggta gcgcatcaat atttacctta tgatttacct
     6601 tgggttctca aacgattttt taaaagaacg agacctcgtg tcggtatcat tatggaaact
     6661 gaattgtggc ctaatttaat taaccaggct caagcttcag gagtggcatt atttctggcg
     6721 aatggacgct tatctgatcg ctccttgcaa ggatatctaa agctcaaatt tttatttaaa
     6781 ccggttttga atcaattttc aggtattttg actcaaagca atgaagatgc tgagagattc
     6841 attgccttgg gtgcaaatgc cgatttagtc catgtattag ggaatatgaa gtttgattta
     6901 cagattaact ctgttgatcg tagtcaatat agggaattaa aaagtcattg gggagaggat
     6961 aggcctacca tcattgcagc aagtactcat gacgacgagg agtctcagat tttatctcag
     7021 ctacctcgtt tgcaggaagc cattccggga gtggtgttat taattgctcc gcgtcatcca
     7081 gaacggtttc aaacggtcta tcagttgagt gttcaagcag gatttaatac tgggtgtcgt
     7141 agtaatttga atacgatttc cagagaaaat gaagtcgtta tactggacag cttgggcgag
     7201 cttttgggat tttatcaaat tagtgatttt gcttttgttg gcggcagcct tgtcccggtt
     7261 ggtggtcata atgtattgga gccaatcgcg atgaatgtgc ccgttttaag tgggaatcaa
     7321 gtgcataact ttaaatcaat ttgccgagag cttaaagagg cccaggcaat tttgctagtg
     7381 aatcatgtca atgagttggt tgatgcaatt atcaagttat atcaggatag ggaatctcaa
     7441 aacactatgg ttgcaaacgc cagttcagtc cttgaaagta ataagggttc tgtagtgagg
     7501 tatttacaga aaattgagtc agctttggga tattagcttg ttataaagtt ctctctatgt
     7561 tgatttcttg tatcatacca aaaaattcat caaaacattt ttgaacttgt tcaaggtgga
     7621 gtatccacgg acaatggcct gctttataaa taactttatt aatcacattt tggcattgaa
     7681 aattcttatc ctgaataaat attgcaggtg gacaaatgta atcaagttca ctggcaatgg
     7741 tcatggttgg aatgctttga ggatgccact tatagtgaaa atcattataa aaatgctcga
     7801 tggcataatg ataggattca ttgttgtagg caaataattc catcatttgc tcaccgattg
     7861 gtagctcttc ctgagtaaaa caataatgtt tataagtatc ccaaaatttt ttataggttt
     7921 cgttggatgg atataaatga tattggcttg cagcagggac taaatccggc aaattatgtt
     7981 tttgacgcat ttcgttaaca tgctgaaaga agctgttggt tgttgttgta ttcattaaaa
     8041 caagcccgct taaatgattc tcaatttccg gaaaattcag tgtgaacatt cctgaaaaac
     8101 tatgggtaac aaggattggc ttgttcaaag atttcagtaa tgaaatgagg cctttctgcc
     8161 aagatacaaa atcaagttgg ccttcactat tatttccatc ttttggaaaa tcaacaaggc
     8221 ttaccgtgcc ctgcaaagtt aacttgtcgc aaaaactggc taaatattca gaaccaagtc
     8281 ccggtccacc tggcagaaac agccagtgtt gatccgagcc ttttttgatt tgatttaatc
     8341 tgtagccata attggtaaaa tacataaaaa acatcgattg cttgtaataa taaactcccg
     8401 cttggcggga gtttattgtt taatgttcag aggcaaggaa gaatcaagct ccctgatttt
     8461 ggcgttcctt gatttcagat agagttttac aatctatgca taaggtcgca gtaggtcttg
     8521 cttcaagacg tttcaggcca atttcgattc cgcaggcttc acaatatcca aaatcttcat
     8581 ttcttaatcg ctcaagagcg tcttcgattt ttttaatgag ttttcgttcc ctgtcgcggg
     8641 ttctcagctc aatgctaaac tcttcttcct ggcttgctct gtcggaaggg tctgggaagt
     8701 tggctgcttc atctttcatg tgcgagacag tacgatctac ttcttccatt aaagattggc
     8761 gccaggcgag caatattttt tctatatgtg ccagctgttt ttcattcata tattcctcgc
     8821 cctcagtctc ttggtagggc gttattccca tatttcctat tgcatcgttt ttcacgttgt
     8881 ataatctctc tttggtttta tcaattaatt gtcctgtctt gccagaccct atatttcatt
     8941 ctgtatctga aactgagttt tatacaaaat cgctacgtat accaaaaaac gtcagtatca
     9001 tcaacaaaaa aataatcact acaaaggttt gttattccga ttgcttggac gttgtcctcc
     9061 acttacccta tcatgtcctt gaagatcttg ccaacagtgt atgtttttat atcctaattg
     9121 attaagtata gcgcttatct catttttttg ttcaaaacca tgttcaacca agagcagtcc
     9181 atcaggcaaa agatattcat aactgtgctg aataatatat tgtagatcag ctaatccatc
     9241 ttgactactg actaatgcac ttgttggttc aaagcggaca tcgccttgtt ttaaatgagg
     9301 atcattttca gcaatgtagg gcggattaga tacaatagcg tggtattgtt ttagggggag
     9361 attattaaac cagcaggaat gataaaaatt aatattgttt aagccgagtg ttttagcgtt
     9421 ataacgggct aactccaatg cctctttgct gaaatcacag gcgtcaatat gccaaagggg
     9481 tctttctttt gcaagcgcca gggcaatagc accacttcct gttcccagat caaggacaga
     9541 aacattctcc ttgtcaggta ttagctccag tgcaagttcc accagacgct cggtttcatg
     9601 tcttggaatg aggacattgg gtgttacttt taacgaaaga gaccaaaatt cccgctgtcc
     9661 agtaatgtag gctattggta gaccttcagc acgttgttta atcatctgca aataggtttc
     9721 tatctgttca ggggaaacca aggcatccgg atgggcaaaa agataggccc ggtttttgtt
     9781 taacacatgg cagagtaaaa gttctgcctc taacttggtt tcctggtttt tgactttgaa
     9841 ttgttgcaga gcttgttcca gtaatgaacg aatatcaatc atgtcgacct aaatcggcta
     9901 gcagttcagc atggtattcg cgttttaacg ggtcaatgac cagggataaa ttcccttcca
     9961 tgatgtcact aagctggtag atagtcagat tgatacgatg atcagtgagc cgtccttgag
    10021 gaaagttata agttctaatg cgctcggagc ggtcacctgt accaaccagt gacttacgtg
    10081 tttgagcttg ttcttgtttt tgtttgctta cttcggcatc taataacctt gtttttaaca
    10141 aggacattgc tttggcacgg tttttgtgct gtgaacgctc atcctggcac tctacaacca
    10201 ctcctgtagg gatgtgtgtg atacgaatgg cagaatctgt tttgttaaca tgttgtccac
    10261 cggctccaga agatctgtaa gtatcgatgc gtaagtcatc gggatttatc tgaatgtcat
    10321 taatttcatc cacctcaggc atgatagcaa ccgtgcatgc ggaagtatgg acacgacctt
    10381 gcgattcagt ttcaggtact ctctgtactc tgtgagctcc tgattcaaat ttgagttgtg
    10441 aataaaccgc ttgcccactg attttggcaa ttatttcctt gtagcctcca tgttcaccat
    10501 ggctggcgct aattaattca atttgccaac cctggctttc tgcataacgg ctgtacattc
    10561 gaaagagatc cccggcaaat atagctgctt cgtctccacc tgtgccggct ctgacttcaa
    10621 gataaatatt gcgttcatca tcaggatctt taggaatgag atgccattgt aattgttcat
    10681 ccaattcctc aatttgtttc ttgacggtgt ctatctcttc ttcagccata gtcgctaaat
    10741 ctttatcatc actttccaat aattcgttta aagaatccaa gttatttttt gcctctaaat
    10801 acgactcata acattgggaa actggttcaa gttgggcata ttcttttgat aaagacttaa
    10861 attgattttg atcggcaata atggatgctt cagacagtaa gcggcctact tcctcgtatc
    10921 gctccaacat ttgctgtaat tttaactcaa gagatttctt catataatga ttgattggct
    10981 gttgtatcaa agagatatcg cgcgaggtca agtaagtctt ctctgttatc ccatgctatt
    11041 tgtcttaaac ctatggtggg attatgagtt agcttgttga ccaagcgcat actgaattca
    11101 tttaaaacgt cttgctgatt ttgaccagcg gatatttttt tcaaagcacg ttgtaattcc
    11161 tgttgagcca aagtgtgcat ttgattgcgg taatcgcaaa tgacgtcttt ggctctaagc
    11221 gaacgatgcc atctaatgta gttatccagt tcgctttcga tcaattgttc tgcttgtaat
    11281 gccgcatttc gcctttcatc catccctttt tctatcatgg attgtaaatc atctacatta
    11341 tagaggtgaa cttgttccaa ttcattgaca tttccttcaa tatcacgagg aacggccaaa
    11401 tccaataaaa acatgggggc atggtttctt tgttccaagg catgctcaac caggctttta
    11461 ttgatgaatg ggagtgggca tgctgtggca gaaataacaa catcggccag aggtaaatat
    11521 tgcgggatat caccaatgga cagtgttttt ccaccgaaag tttcagctaa tttttgagca
    11581 ttttccagag tgcggctcgc tatcaaaaaa cgatggacac catgctgatg caggtattta
    11641 gcaaccagag aagctgtttc tccggaacca attaaaaaga cacttaagga atgatagttt
    11701 ttaaataatt gccctatcag ttgtaccgca gcataggcaa tcgatacggg attagctcca
    11761 attccgctac gagttctgat gcgtttggac gctctgaaaa tatattcaaa tacaggccgt
    11821 aattgtgttt ttactgtgcc aagacgacaa gcatgttgat acgcctgttt catttggcca
    11881 aggatttgtg gttcgcctat catcatggaa tccaaaccgc tggcaacacg caaggtatgt
    11941 ttgattcctt cttttccctg atggatatac aaaaattgag ataataattc ctctgataat
    12001 tggtgttcat gagctaacca gtttccaaga acctcagggg tattggtatc acagtagatt
    12061 tcagttcggt tgcatgtaga taaaatagcc gcttcattga cttcaggtaa atcaagcaga
    12121 ctgctcaata gagaatcttg catggctggc tgcagtgcga ctttttcacg cacatgaatt
    12181 ggagcagttt tatgattaag cccgcaagct acaaatacca tagaatcttg atttatttgt
    12241 ttaagggaat taacgaattg taaacgattc attgtaaaat ttgtagagaa aaaggcaagg
    12301 ttccttgtat ttctgtttct tgcaatcatt ttacagtata ttttttaccc tccttcttcc
    12361 tcaagattat cccacttggg aaaatttgct ggcaagggat tcaatttact ttctctatct
    12421 tcaaacgggc tcatatctaa taatgagaac agtgactttc taaagtcctg ttgagttttg
    12481 ccgccaacat gtatagccac atattgcaca acatgtgtca tttcaatagg agctttaaat
    12541 ggacattatc ctgcaaggtc aacataatgc tattgaaact gttgagagcc tggagagggt
    12601 tctgaaatta tttaaagagc gttaccaggt tgatggattt cgcgagattc atttgactgt
    12661 cactttagtt gatgaacagg gcgatgaggt ggagttggtt gatagtgata cgaatcaacc
    12721 ttatcgaatt tttgaagttt atcgtaaagg atatgaattg gctggaagaa aagggattcc
    12781 aatgttacag ctggtagttg ataatacgca caaatagatt attctttctg gtgtgttttt
    12841 aataagcctc atattctgga taaggagtcc attatgatta tggcattggt agccataata
    12901 cttacgttaa tcctggtgat aggtatacac gaactggggc atgcactggc agcccgcctg
    12961 tttcaggtca aaattagtaa aatttccata ggatttggaa aaccattaat tcaatggcaa
    13021 acgcaaagtg gttgtaactg gatctgggca atgtggcctt tgggagggta tgttcagtta
    13081 ctgaattcgc gcattagtcc agtaaagcca caagaaaatg cttattgttt tgataagaaa
    13141 ccaatttgga ttcgtgtgct gattttattg tcaggagcga ttgccaacct tattaccgca
    13201 tggatagcct tggtgttggt tttttatatt ggcattagct acaaacaacc tcaaattcaa
    13261 tcggtaaaac tggatagcct tgctgccaag gcaggtattc aagcaggcga ccaatgggtt
    13321 tctgtagaga attacccaac tgattcctgg caaggggtag gtatgcaatt ggtaattcat
    13381 tgggggcaaa agaatgttca cattgtttta aggcaagcga atcaacaatt aaaacaattg
    13441 agtcttgatt taagtcagat tgaatttact tcaaaagatg gctctttgct cgagagtcta
    13501 gggatcaaac ccgatttgtc agtagtttcc agtttgacgc gctatccatc tctactggct
    13561 tcaatgcaaa aagcgtttgc tgagataatc cataccatgt acttttttat aatgatattg
    13621 aagcaattgt ttctcggggt tattcctttt tcaattttac ttggaccgtt ggctattttt
    13681 tctgtttctg tggcttcttt aactcaaggt gttatcgttt ttttactatt cattgctaca
    13741 ttgagtcttg ctgtagcttt ggtcaattta tttcctatac ctggtcttga tggaggcagc
    13801 attttatatt cggttataga gaaaattcgt gggaaacctg tctcagtggc tgtggaagtc
    13861 ttgcttcatc gcctgatgat tattctattt tgtgttttgt tagtgcattt attaatgaat
    13921 gatttgaatc gatatttaca ccattgacag tggactcata ggaacgggct gccttttaat
    13981 tcttagttac agcccgcgca caacaacata tatctactct cttaaccccg gcatttttta
    14041 atgtatgtgc tatttcattg gctgtgctgc ctgtggttaa taagtcatca acaatcatga
    14101 cgtgttcata agttacaggt ggtacataaa aagcatgacg taaatttttt cttcgttgct
    14161 ctccatcaag gttggcttgt gaagcggtat tgattatttt ttggcaataa tataaatcat
    14221 aagggatgtt taattgacga gctaataatc tggttaatac agcagcctgg ttaaatcccc
    14281 ttcgttttaa tcgtttggga tgcatgggaa cgggaattag acaatctggt ttcaatgcgc
    14341 ttttgggtaa ggcattaaga agtaattgtt ttagaaaaga ggccagataa aggccattat
    14401 ggtatttaaa ctgatgtatt aaactacgca gaggctcttc aaaacgatac gctatataag
    14461 cactatcaaa ggagggcctt tttctaacac attgaccaca aaccagatac gtatcatcag
    14521 aaagggggta ggcacagtac tgacagcaag atccaagttg tttcataaac tccatgcaat
    14581 tagagcaaac tgcaagttgg cttttgtgga actggttgca aagagtgcat attgaaggta
    14641 aacgtaagtt ctgtgttata cttaagaatt tttggagcac gatagttgga tttccagcat
    14701 ttaatttttc cctggtcttt ataatgacgg tcaacaatga aataagcaag gcatttagca
    14761 aacatgccga tgactatgag cgtgtggcca aagttcaaaa agaaatcggc agtcgtttgt
    14821 ttgagcgatt acaatatttg aaaattgctc ctcgacgaat tcttgatttg gggtgtgggc
    14881 ctggattttt ttcaaaagaa ttggctttgt tatatccgaa agcgcagatt gttggaatgg
    14941 atttgtcgtt tgccatgtta gagcaagcca ggaaaaaaca gggttggcgt cgtaaatggc
    15001 ctttagtcag tgccgatatg cagaaaatgc cttttgcaac aggcgctttt gatttggttt
    15061 ttgctaacca ggttattcac tggagtagct cattaggcac agtgtttcgg gaacttaatc
    15121 gtgtaatgaa tgtcaatgga tgcttgatgt ttactacctt gggtcctgat accttcaagg
    15181 aattacaaac ggcctggtct gccgctaatc aatatgcaca tgttaatgaa tttgttgata
    15241 tgcatgatat tggagattgt ctaattgccg agcatttcat ggacccggtc attgacatgg
    15301 aattactgtc tatacattac gagacccttc cccaattgct attagctcta aaaactcaag
    15361 gggtaagaaa tattaaccct aaacgaaatc acgggctcac aggtaaaagt gcatggaaac
    15421 aatttgaagc acaatatgct actatgcgga ctactaccgg caaatatccc ttaacttatg
    15481 aagtggttta tgggcaagcc tggaagggtg cgcaacgtaa aatggatcaa ggtatagaaa
    15541 cctggattcc tgtttcacgt gttgttaaaa agtcctaatt taaaacggct atcaattgga
    15601 ttgtctggtg caccaatggt gtcaagacaa attgctacga ttttgtaatt gtttggttaa
    15661 ttcaattaat gcagccgctt tactaccaaa taaccgtaga gaatccattg caatttggta
    15721 atgaacagcg atttcttctt ccaattgttg cttgttaaat aaggttgcaa aggtggtttt
    15781 ttggtttgcc tgatccgagg agcgaccttt tcccaatatt tgagttggag cgtaaagatc
    15841 caggtaatca tcttgcattt gaaatacaag ccctatatgt ttcccataag tttttagtgc
    15901 ggatttgatt tgctctgata cctcattttg tgctgctaaa accatttcaa aacacgccag
    15961 gatcagtttt ccagttttca aaagatgaat ttctcttaat tgctcttctg ttactgataa
    16021 tttggccagt tctgataaat caagactttg gccactaacc attccactta ttccactggc
    16081 atttaccaat acttgagtaa tggctacgac ttgtgctgcg gggagaaggg gcgataatct
    16141 cgtcaatacg acttcaatcg ctaaagcttg catgccatcg ccgactaaaa tagcggttgc
    16201 ctcatcaaat gctttatgac aactcggctt accgcggcgc agatcgtcat tgtccatcgc
    16261 cggaagatca tcatgaatca gggaatagca atgtgttaac tcaagcgctg ctgcaataat
    16321 atccagaact ccctggtcaa catcaattaa atcgccagct aaataaacca aaatgggtct
    16381 tattcttttg ccgcctgaaa ataaagcgta atgaagtgcg gagcgaattt ttggagctgg
    16441 tattattatt tcattgagca attgctcgag atatagctca tgtctttggg tatacttatc
    16501 aatcacttgt ttgttcatct gagtcgagtt caatgttgga atcagtacca gttaacgttt
    16561 caattttttg ctctgcctga ttcaaagcat tttggcaacg tcttgcaagg ctaattccct
    16621 tttcaaattg tttcagcgat tcttcaaggg taagctctcc cttttccagt tgccggacaa
    16681 tttcttcgag ttcagtgatt gattgttcga aatgtatgcc tttgctcata actgttttcc
    16741 aaggcgataa aaggtgtgat tatactgggt taacgcgagg attcaacttc tgaatggaag
    16801 taagtggaaa atcaatcata gaagcttatt ggcagttttg ggttttataa aggaactcca
    16861 gaactggatg ttggactttg ctagtttaat atcaaagacc aacattaggt ggttaacagc
    16921 gtctaaagac caaatgcttt atcaaaatcc agatcaaatt cttcatcctt tgttttgtta
    16981 ttctttgtag tgaagaaggt ggggctgttt tttatagatg tctcatgttg ttgaatctga
    17041 tcacggcatt tatcttttaa cgcgagcatt tcttcatata aagagcgtat ttcaattttc
    17101 aagcgatcaa ccacttcact gccgcctttg taaattgtgg ttagagaacc cattttgaca
    17161 aagtgttctg tgtgggcgtt ttccaggaac tttagttgag ctgttaaaga ggaggactct
    17221 tttgctttcg ctatttggtc ttccatatac tcttttaatc tggtaagttc tgttaccgga
    17281 ttttgtcctt ttttgaaagc aggcataatt ttgacctcac attttaaaat aatcccttag
    17341 gtaagttaaa gcaaaaacag gcaattgaaa agcaaatatt ttcatattct tttttattag
    17401 ataagtatta acgcatagtg cctggtatag ggttaatcaa tttaaaatga agtcaaattg
    17461 tttgtcaggt aagtctatgg aaatcaggtg cgatttgtat aaaaaaacct gacattcgca
    17521 ttgaaacatt gtgaagccaa gcgaatgtca ggcttttgtc ttaggtttaa ttatttgggt
    17581 gatgaagccg cttgacgaag ccccaaatct tcttcagctt tttctaccgc tttggctgaa
    17641 tctggacgtg cataagcgaa gaaaccttgt tgacaaccaa acgtaaagac ggtaatcacc
    17701 gcgttgccca agcactttaa aagatttttc aggtagtcac cccagcctaa gtctttttct
    17761 aaaaccggtt tagctatttt tacggcatct tgacaagcta atttaaactt tcttccagca
    17821 agttccggag aaaattcatt ggctcttaag tctcgctcat actcatcacg agcagctata
    17881 agactttgga gaagtgtatt tgctgtgtca tgtgcttttt tatactggtg ttgatcaact
    17941 ctgtctattt tgagtgtgag ctcttgaata attccatcaa ctttctggag agcttgagtt
    18001 atagtttctt cttgttgatt ttcaagctta ggtttttctt cttcagattg ttttgcaagt
    18061 tttagttttt cttcttcaac ctgtttttcc agcttcaatt gttcttcacg cttaagcttt
    18121 tctacttcag cctgtttgcg ttgttcttct aattttgttt gaacttcact aaccagtgtc
    18181 aatggatgct tatggctgaa ttgatcacgc aaaggctcat aaagttttct tacgtcaatt
    18241 tttagctgtt caacttgctc attgccacct ttgtaaactt ttgacaggga acccatttta
    18301 tcatcgtatt cattcaaagt gtctatgagc agttgatcga tgtcctgttc tgaggttgct
    18361 tttttaattt ttgaaatttg ctcttccatg tatgccttaa gttcagttaa acctttaact
    18421 ggatcctgac ctacggtgaa ttttgccata attttatcct cggtttttat aaaaaaatca
    18481 cttcggcaaa taataaagca agatagtgtt tttgaaaagt aaagttttaa taaatatttc
    18541 gtaaattgag cgcaataaaa agaaaaatgg ggtgttcaag ataaggtaag ctctggttca
    18601 tttaaaaatt atttaaggca aatatgttca gcgcgagtta tcataaatgt actaaataaa
    18661 atgcttgaat caaggcaaaa attatgaaag tgaaaaccca gtttgtttgt aatcaatgcg
    18721 cggctatttt taaacagtgg gcaggacaat gcactcaatg tggcgcctgg aattccattg
    18781 ttgaagaagg gattgtgcct gaaaacaaga cttcacgaag tgggcaatgg gctaatcagc
    18841 gctctgttat cactgctgtt gaagacgtcg tcatggacaa aatagtacgt atggattgtg
    18901 gtatatccga gctcaaccgg gttttgggag gggggctggt ttatggctcg gttgttttga
    18961 ttggaggtga tccggggatc ggtaaatcca ccttactgtt gcaaacgctg gctaatttat
    19021 ctatgcaaga aacagtattg tatgtgacgg gggaagaatc attacaacaa gttgccatgc
    19081 gtgcccaaag attgggacta cctttggccg gattgcggct attggcagaa actcaggttg
    19141 aatccatcat tgctcaagcc caaaaggaaa aaccgcgaat tattgtcgtc gattcagtac
    19201 aaacgatatt cactgaagcc ataagttctg ctccgggagg agttagtcaa gtcagggaat
    19261 cagcagcaca attggtgcgt tatgccaaga tgaccaatac ggccatcttc ctggtagggc
    19321 atgtcaccaa ggaaggaaca ttagccgggc ctcgggtgtt agaacatatg gttgatagtg
    19381 ttctctattt tgaggggcaa aatgatagcc gcttcagagt cattcgcgcc attaaaaatc
    19441 gttttggcgc tgtcaatgaa ttaggtgtat ttgcgatgaa tgacaaaggc ctgaaggagg
    19501 tagccaaccc ctcagctatt tttttatctc gccagcccga acctacgcct ggcagtgcag
    19561 tgatggtgac ttgggaagga tcaagaccca tgttggtaga agtacaagca ttggttgatg
    19621 aggcccatgg tcagcaagcg aaacgcgtta ctgcgggtct tgagcataat cgtctggcca
    19681 ttttgttagc tgttttacac cgtcatggag gtatcgctac ttacgatcag gatgttttta
    19741 tcaatgtagt cgggggagtg aaagtgactg aaacagcgag tgatttggct ttgttggctg
    19801 cagtgatctc aagtttacgt aatcgtatat ttgatagtga aaccatcata ttcggtgaag
    19861 tgggattagc tggggaaatc agaccagtgc aaagtggtca agaacgcatt aaggaggcta
    19921 taaaacatgg attcaagcgt gctatagtcc cttttgccaa cgctcccaaa cagcaactcg
    19981 attccatgac ggtagagcca gttaaacatt tgcgagatgt attggagaaa atgtgagtta
    20041 tcaaaaacca atcacaccgg aatataaaat gaatgaagat actatccgtg attttaatcg
    20101 tgatggtgcg gtatgtcttc gccaattttt aaatgccgaa gaaattgcta ttctaaaaga
    20161 aggaatagaa tttaatttat cctccttaag tgcccgtgca aaaatagcca gcagacacga
    20221 tgatcctggt tattttgttg aggatttttg tacttggcaa aacaatcctt cttatcaacg
    20281 aattatcttt ggtacgaatt tgggagtagc tgccgctcaa ataatgcaaa gccaatcagt
    20341 ccggttgtac catgatcatt tgttggtaaa ggaaccaggc acacgacagg ttacaccctg
    20401 gcatcaggat cagccttatt ataatattga ggggaagcag aactgcagtt tctggattcc
    20461 ggttgatcct gtcagtcgtt cttcaactct ggaatttatc gctggttctc atttgggtcc
    20521 ctggttaatg ccaagaacat ttttagataa tcaggcaaaa tggtttcctg aaggaagttt
    20581 gcaggagttg ccccctattg aagaggcacg cgcaaggtat ccaattattg gatgggaaat
    20641 agagccaggc gatgttgtat gtttccatat gctgacttta cacatggcaa ggggagttga
    20701 gggaaggcat agaagaagag tattttctgt gcgtttttta ggtgatgata ttacgcatgc
    20761 ccccagggca tggcctacct cacctgattt tcctggtttg acggccgaaa taccggcagg
    20821 tgcaagcatg gatcatccat tatttccatg tttgccactc aaaatatagt aaaacgatat
    20881 atgttatttc ccttcaaaaa agagttcatt tttcaaggat gagattttta agattcctat
    20941 tcacaacgga tgtgattcca ataagcgtac aatcaaaagg gtatattttt tatcattgac
    21001 taccaaaatt cgttattgtt ttccattaag tattgtattg tatttttgaa gtgtctttat
    21061 gtatacctga tcgagaggca ctgttcatgg tacatcggtt tgctctatta aacttatgtt
    21121 tgaccgtttg aggattaata ttacaaggag taattatggc gttacctacg gagttaaaaa
    21181 gcaagttcat tcaaattctg aatcaaaata aaatttctca ttctgttggt tggtctaact
    21241 acatggagag tgttaataat ccaaccgcga ttgtcgtgga agttaaaaat gaaaaagagg
    21301 ttcagacagt gatgaaggcg attaaggaat tgaattctga cagggaaccc aaagataaag
    21361 tgactgtgag agctactgcg ggatggactg ataaacaggg taaaagctgt tgcttttttc
    21421 catggagcga agctcaggag agtcagtaca atgaaggatt ttctttttcc caagttgtag
    21481 gtggtaaaga gtctcatgat accgcaggta ccgatgtcat tattcggttt gcaaaaaaat
    21541 ttcaccatgc aaaagtgatt ggttcattaa aaaatccacc tgtctttaat ccggacaatc
    21601 ccattcatag actccccgtg actttggtgg aagtgagcgc tgggatgcag gtggctgatt
    21661 ttgccgattt tttgcgagaa catcagttat ccttgtcaac tgttagcatg atttcctggg
    21721 tgacccctgt tggcttggct ggaacagcgg gacatggtac cgggcgtgat gaaccagctt
    21781 tcagtggcct aattgaatcg gttacagttt gtgatatgga tggcaacctg cgtgaaatta
    21841 attctgatca tcctgatttc aaagatttga caggtggtca tagcgggttg ttgggtattg
    21901 ttgttagcat gaaattacga gcggttaagg catttaattt atgtgaaaca atagagttat
    21961 ttcccaatac cagggaaatg acaggcaggt taagtgatat attaaagaat aatcaataca
    22021 tcagtattat gggaatgcca agttacggct gtcctgaatc ggataaactg gtttccaaat
    22081 ggcaaattcg tcaatggaat tatactaccg acaaaccagg gaaaaaggaa aaggcacctt
    22141 atgatccgga cgttcgctct tttgctcaag agttacaggt tcgcattggt gcctcagtga
    22201 tggatttctt actggactca ggattaaagc atttattacc ttcttttatg ttactgtcag
    22261 cagcagtagt cacgggtact cgtggtacgt caccaaaggt agattatgaa aatcatatca
    22321 cacaccctca ggtggctttt ccaaaggaga tgagggatgt gagttatttg attccagtca
    22381 aagatgacga agcagggcaa aaattggaac ttattttaca aaaaatggaa gccatgttga
    22441 acgaagcggg tagtaaaggg gaatttcctg ttacttatgc gatttatgtt cgttatctta
    22501 aggggactaa cggaggatta tcaaccagtt ttacttcatc agataatgag cgtattttag
    22561 cgatagacgt agtcactcat cctgaagcgc ctgggattac aaattttgag aagggattca
    22621 tgtctttttt aaaagagatg aatattattc ccagaaatca tttgggaaaa aatttccccg
    22681 cgggcgtagt gcattatgat cagtttttgg gggcggagag ggtgtcaaag tataagcagg
    22741 cactggagag attctattgc agtccaggaa agaacgatgg tgctgagcgc ctgagcatgt
    22801 ctccttttta taccccttat ttgcaaggca tgttatctgc aagtgcacct accaagcaat
    22861 tagttgatga aaataaatac gagcaacgca tagagaggtt gcatgaggag cattctgatc
    22921 tcgaatgtgc tgagtttttg agtaaattat atgccgaggt gtccaggatg agtacgtctt
    22981 cagaagaggc tcttactgca aaaaaagggt ttttagaagc atgtaaggcg gaattggagc
    23041 acaggaacag tcgattagaa gcccctgtac ctctattgag ttaggcaatc aggaaaataa
    23101 gtacaagacg gagcctagca aattcaggag tctctactaa acttaaaaat ataataatat
    23161 tttgagttaa cctatggata ttactccttt ttttaagctc atggtcgata aaggtgcctc
    23221 agatttgttt ttcagtgtcg gggcaccgcc aaatattaaa atcgagggca tcacatcacc
    23281 tattggccag gttcctatca aatcgaatca aatggcagaa attgttttat ctattatgaa
    23341 tgacgcacag caaaaagaat ttgagtctaa aatggaactc aatatggctt tttcattagc
    23401 tggagtaggg cgtttccgaa tcaatttatt caggcaacgt ggcgaagtgg ctatggtggt
    23461 gcgttacatc aagggcgaaa taccttctat agaagattta aatctccctc aaattttaaa
    23521 gtctctggtc ttggagttac gaggtttagt attggttgtt gggtccacgg gatcgggtaa
    23581 atcaacgact ctggccgcga tgatcaacca ccgtaatgaa aatcacagag ggcatatttt
    23641 aacaatagag gatcctattg aatatataca tcaacataaa aaatcaatag ttgatcaacg
    23701 agagataggc attgatactc taagctatga caatgcattg aaaaacgcaa tgagagaagc
    23761 acctgatgtt atcttaattg gtgaaattcg tgatcttaac tcaatgaaac atgcgatagc
    23821 ttatgctgaa acaggacatt tgtgcatttc tactttgcat gccaataacg ccaatcaggc
    23881 tctggataga gtgattaatt ttttccccga tgaggcaaga caacagatat tattagattt
    23941 atctcttaat ttaaaagcaa tcatttcatt acgtttgata ccaggagttg ataatcaacg
    24001 tgtcgcagca gtggagatct taattaactc tccttatatt tccgatttga ttgaaaaagg
    24061 gaagatagac gatattaaag aagtcatggc aagaagcacg gaacaaggca tgcagacttt
    24121 tgatcaggct ttgtttaatt tatataaaga aggaaaaatc accaaagaaa atgcgattaa
    24181 gtacgctgat tcaaaaaaca atgtgggttt acaaattcga ctgggtgaag aaaaaaactt
    24241 caatattcaa gatgagaatt tacaaataca acccgatgat attgataaga tttaattcac
    24301 tatgcatttg tt
//
LOCUS       DAXLDT010000039        24223 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXLDT010000039 DAXLDT010000000
VERSION     DAXLDT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24223
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.039"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1326)
                     /gene="ankH"
                     /locus_tag="ACF2SP_001207"
     CDS             complement(<1..1326)
                     /gene="ankH"
                     /locus_tag="ACF2SP_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkH/LegA3"
                     /protein_id="HFD2359001.1"
                     /translation="MSIANDIISHRMPDFDAYLRAGESLDDIDEYGFTPLIECAITRQ
                     IKIAEQLIARKVDINKPDVTGRTPLHWAVDNNDLDMTKLLLTYGADPNAYTRNGLCVL
                     VYPVLRGQDAIKQLLYHHGAKLDFALDFINAKLIGHRFELQGDVDIVNAKGEFIELDY
                     EGFILEFTVAVVKDSLRRFISSYSTRHLRDYFPYIHNIMDAFTDAAELLQYQHHPRLG
                     EQHFKRIAALLKAPMLILPAASRGHALCFVRYHQWWAKIDRGENSLQEGSVNIYRITR
                     PEALTINFIHDFLYKKQNRRYYHQMINQQLGLLPFAQMPISSQISGNCSWANVQAVVP
                     VAYSMQELTSVENFKPDVALSLYDEWVEWDKDRALDECIQRFYLASPERKASIAAILG
                     GILFQACDHANKRHLERAEKILNILILDEYYYVLSSYLEEYCIKRLTRKG"
     gene            complement(1327..2100)
                     /locus_tag="ACF2SP_001208"
     CDS             complement(1327..2100)
                     /locus_tag="ACF2SP_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFD2359002.1"
                     /translation="MAKEYYLSDEIEEIVLASTVLSYSRPNLSSKRFNDLKNGKTPWE
                     AELDLNGLNVESARKSLYQFIQTQIKNNKQCVLIIHGADNSQDKPPLMKNLVNRWLPQ
                     INEVLAFHSAKPKDGGSAAVYVLLGSVFHVELPTRLPSNRSESTFLVAETKAMERRRR
                     LAEQQGERRFASVKAREHPEEEIKVAPEGELQNNILQHPELNSQRFDGIDPNLNPEPP
                     LNTEARREFDNERREQDKEKQLRLGNMPKFTTAPTPRGP"
     gene            complement(2248..3270)
                     /locus_tag="ACF2SP_001209"
     CDS             complement(2248..3270)
                     /locus_tag="ACF2SP_001209"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFD2359003.1"
                     /translation="MSRHLNVAIVGATGAVGETFLTVLEERNFPIKSLYPLASSRSVG
                     KTVTFRDQELDVLDLAEFDFSKVDLALFSAGGAVSKEYAPKAVAAGCVVVDNTSCFRY
                     EDDIPLVVPEVNPHRIADYTKRGIIANPNCSTIQMVVALKPIYDAVGISRINVATYQS
                     VSGTGKKAISELVAQVGDLLNGRPANVQVYPQQIAFNALPHIDQFEDNGYTREEMKMV
                     WETRKIMEDDSIMVNPTAVRVPVIYGHSEAVHLELKKPLTADDARALLAKAPGVTVVD
                     NLSKASYPTAIKNAVGHDDVFVGRIRQDISHPCGLNLWIVADNIRKGAATNAVQIAEI
                     LQKEFL"
     gene            complement(3380..4438)
                     /gene="aroC"
                     /locus_tag="ACF2SP_001210"
     CDS             complement(3380..4438)
                     /gene="aroC"
                     /locus_tag="ACF2SP_001210"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFD2359004.1"
                     /translation="MSGNTFGALFTVTTFGESHGPAIGCVVDGCPPGMSLTEADIQPF
                     LDKRKPGQSKYTTQRREEDKVQILSGVFDGKTTGAPIALLIQNTDQRSRDYEDIKNLF
                     RPGHADFTYHYKYGHRDYRGGGRSSARETAARVAAGAIARLYLKRYLNLDIIGYLQQM
                     GDLKLQFENENEINKNPFFCPNNKQTQELADYIDRLRRQGDSVGARVKILARGVPTGL
                     GDPVFDKLDATLAYAMMSINAVKGVEIGAGFNAVEQLGSYHRDQMTAKGFLSNHAGGI
                     LGGIATGQPIEVSIALKPTSSITTPGQTINTEGEEVTVVTKGRHDPCVGIRAVPIAEA
                     MMALVLMDHYLRHKAQCK"
     gene            complement(4450..5382)
                     /gene="prmB"
                     /locus_tag="ACF2SP_001211"
     CDS             complement(4450..5382)
                     /gene="prmB"
                     /locus_tag="ACF2SP_001211"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFD2359005.1"
                     /translation="MVMVDTLVSSYFSKETEELVTILDFLRFSLSCAENAQLFYGHGT
                     DNAWDDMRSLILRSLSLPYDVDSFLLNARLTTSERKHLCNQLDKRINQRVPVPYLIKE
                     AYFCDLPFYVDERVLIPRSPIGELINNQFSPWIEAERVHHVLDLCTGSGCIAIACCYA
                     FPEAQVDAVDISNQALAVATINCERHDVGDQLALIESDCFTALSGKQYDLIVSNPPYV
                     GKEEMQTLPDEYRHEPVLALETGNNGLAIIEKILKNAHAYLSEHGILVVEVGNSEEAL
                     CETYPMVPFTWLEMSNGGQGVFLLTKQQLEDYFK"
     gene            5547..6116
                     /locus_tag="ACF2SP_001212"
     CDS             5547..6116
                     /locus_tag="ACF2SP_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFD2359006.1"
                     /translation="MTDDFLSDEDKALFRHHMRSVKPLNEKTKRVKSPTQPTHEKKTT
                     TFKSQPVPKEEYFLSDFIRDTVQPDTILSFSEPGIPNQRFRALKNGQIPWEAKLDLHG
                     LKAEAARQALYDFIKTQSLNNKRCLLIVHGKGGHQGAPPVIKNLINRWLPQFSQVLAF
                     HSALPKDGGLGAVYVLLKRNREKDEQGNY"
     gene            6189..6608
                     /locus_tag="ACF2SP_001213"
     CDS             6189..6608
                     /locus_tag="ACF2SP_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFD2359007.1"
                     /translation="MEKLGQFIINHWQLWLAFVVVVFLIFINEILSQRKKAKEVSPQS
                     LVDLMNNGNAIVIDIRDKESFKNGHIINSMNVNPDDFEQQKMSKYKDKPIILVCARGL
                     QSPTLAMKIRNQGYQPLVLSGGIQAWQNADLPLVKGK"
     gene            6613..6867
                     /gene="grxC"
                     /locus_tag="ACF2SP_001214"
     CDS             6613..6867
                     /gene="grxC"
                     /locus_tag="ACF2SP_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFD2359008.1"
                     /translation="MNEVILYTTGYCPYCIKAKELLDRKKVIYTEIRVDLQPELREEM
                     IQKSGRRTVPQIFINGQAIGGCDDLYALEAQGTLNELLKK"
     gene            6877..7365
                     /gene="secB"
                     /locus_tag="ACF2SP_001215"
     CDS             6877..7365
                     /gene="secB"
                     /locus_tag="ACF2SP_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFD2359009.1"
                     /translation="MTEQLNTNQQNDEAQFMIQRIYIKDLSYETPNTPAVFQQQWEPE
                     LKLDLNTTTTQLDKNVYEVVLTVTTTVMNQKTTAFLVEVKQAGIFTIQGAAASQLDHL
                     LHSFCPSILFPYAREAITSQVIRGSFPQLVLAPINFDALYMQQLEEKQKATGAKDETV
                     SH"
     gene            7367..8356
                     /locus_tag="ACF2SP_001216"
     CDS             7367..8356
                     /locus_tag="ACF2SP_001216"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFD2359010.1"
                     /translation="MNKKTIAMLGAGSWGTAVAIHLAKIGHKTLLWSHNPQHVALMAE
                     QHSNPAYLPGIPFPENLIPSDDLIECVQLADYVIIAVPSHAFAEIINKIPKPTQGLAW
                     LTKGVDPASHQLLSQLVASRFGVDFPIAVISGPSFAKEVARFLPTALTLASNNTNYQK
                     KMHQLFHHDNIRVYLSDDLIGVQLCGAVKNILAIACGISDGLGYGANAKAALITRGLA
                     EMTRLGLSMGARQDTFLGLAGVGDLVLTCTDDQSRNRRFGLLLGREVPIPEAEHQIGQ
                     VVEGKHNAAQICAIANKNKVEMPICEQINALLHGIVHAQQAVNNLMSRPAKEE"
     gene            complement(8434..9273)
                     /gene="murI"
                     /locus_tag="ACF2SP_001217"
     CDS             complement(8434..9273)
                     /gene="murI"
                     /locus_tag="ACF2SP_001217"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFD2359011.1"
                     /translation="MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYL
                     GDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIP
                     VLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSSRACSVLVA
                     LAEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQ
                     ATAISLYELLQNQNLHNDNLEVPPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELID
                     AMG"
     gene            complement(9343..12882)
                     /locus_tag="ACF2SP_001218"
     CDS             complement(9343..12882)
                     /locus_tag="ACF2SP_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interaptin"
                     /protein_id="HFD2359012.1"
                     /translation="MILEEYIRMAKNKEFFDALEEIAVSAKNDETLRDELAKVLDDIL
                     KTDPSDPDAFRKIIAEHQDFWDEHDPSLMEFNEGLFFGPSTEEYLESDDFLDSNDPIN
                     SFQKLHQFAAEQRVKLDLEKSDTDTLVAILKNNPEECRAYIESKKPGLGNFSEGNVHG
                     WLKEEYTPTIPPKAINKSTDVLSDGAIKKIKEQASELLLLKLIKNCENRQLLKDLLTA
                     NSKTEAEHAANAIGFPTEGNGELSYPLSDEVEEALDDIIQELEEEAAKAGFDSYVQSL
                     SHDALLAKKNGLESTTAVGFKNSLDEPYKTYLPESEWQRAQGVLGARYLQAVLSSGTQ
                     NLKDALNAKDANALIAELRKPALLGPHDYIDKTVTEENLGSLKKNMMKSFINNIKDET
                     NLKALDALKALDGAKNLDKFKEVLGKLGITPADWVKDTDLKDMKQWARDRQFELEINR
                     VSSLGSGAHSKLMSTLTQLPVEKQREILAKPQQLRHLMNAYESHVAEHYLGKNASGIA
                     ELLTENKRLEGFRAIHNAEVARVLANFKPEITLNDKQVAAINQALTTANSNPNTYTQV
                     TDYKTLIDAIKTQSGSVNQKDFYNAFNLNDDGSAFTSSTPRKDEMSKQQEYNKNLYIE
                     YNNPANSGNKKLLGVLLSLDKLVIFGIRGKPSTPTSSPITNYFLENLKNSKTVEEYSD
                     NLFGKNPTDPGLKKLKQDCLRELTPALFNEIKNDLRKQELLDTNPANVMTAIKALSTE
                     LESIKGITGPIRTNADKLKFINDIDPVHLYNPTFQGTARSKAAQMKERYEGLSRDCEL
                     VVDQLRRQVIALEGHLKSLPQDSQFKAAGLTLEQKEEIRKLRTELQTELDAVRKDLDF
                     YKKIQGKLETIVKEVDVAAKGKMHYYYNSEGIKRHPPVSRDQIPPLPNVPNPSLRSST
                     TATTGSNGRIQEFLVGEKIPEGQIVVVDVSHKTAPKSGAPVETIGRYTQDNNVPDQVT
                     SKKGEISKVPGSKFEILQFPTQVPPPNPPSGDPLVEAKVNFSMAMAADILASLDSPPT
                     KDKPIRLRGSNPEELEYLYTALVILGEKNPKFKFNRDAIEVNSAVFHPDNVKGRLWGF
                     SSNSLYSQVFTNTGLTETQNIIQSKIKHMQQMTDEKFSPQKEREKVDSKVQEITDKQS
                     KMKKELNPVHKTTERTIEQEGPAPESPSTGMRK"
     gene            13004..13306
                     /locus_tag="ACF2SP_001219"
     CDS             13004..13306
                     /locus_tag="ACF2SP_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359013.1"
                     /translation="MRIKIILCGLILTNLTYAQGCIVGGQASQPRYVCFDGRTLDLTE
                     TGLVWNAKRGCFISSVPCCNYNATHYAYHPNPAKIGGAYARCRHDYPFTLGQMQTH"
     gene            complement(13310..14602)
                     /locus_tag="ACF2SP_001220"
     CDS             complement(13310..14602)
                     /locus_tag="ACF2SP_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HFD2359014.1"
                     /translation="MSPSESGELSIIQELSQRIVEAQRQIRILDSIKWDESVKQEFFK
                     FKGKKLPSIDKAYYDGKPLPFKVKDKQEEFRIILRDAQNQLGQYSTVTRLIRRQCEEY
                     SRAAQMLSLRGTPSFSEMAMELYGSPDDVFYVGGPRMCEMGSLLFDVLKNLQVQLQSQ
                     ADVKRYTPIQAQEILQERLEKFFDKHPGRVSVMVSDDMIADASAGADSIKLSQNAMFS
                     DRDLRYLEVHEGWVHVGTTLNGALQPNCFFLSKGSPSSSVIQEGLAVITEVVTFSSYP
                     GRMLKITNRVIALDKVTQGADFIDIYRYFLECGLSEEDSYNQSVRIFRGSTPTGGPFT
                     KDLSYAKGFVLIYNYIRFAIGQKRVDTIPLLFTGKLVLDDLPLLGELKEREILTDPVY
                     LPPPFQDLAALSAWMSFSLYLNQFDLNEIQKNFRFLLV"
     gene            complement(14612..14842)
                     /locus_tag="ACF2SP_001221"
     CDS             complement(14612..14842)
                     /locus_tag="ACF2SP_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359015.1"
                     /translation="MKKLSFIVLAALALTACNSRYASNGENLYLQSRNGEALVVPPPL
                     TSANISNFYDLPQQTQDARVSIAPPVEDITTS"
     gene            complement(14857..15729)
                     /gene="dapA"
                     /locus_tag="ACF2SP_001222"
     CDS             complement(14857..15729)
                     /gene="dapA"
                     /locus_tag="ACF2SP_001222"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFD2359016.1"
                     /translation="MFSGSIVALVTPMRNDSVDVHHLRELVEFHIAKGTHALVAAGTT
                     GEAGTLSHSEKLLVIKTVIEQAKERVPVIAGTAMNATKDCIELTQQAMEYGAHAALIM
                     TPAYIKPTQEGLYLHYSHIAQSVAIPIILYNVPGRTACDILPETVARLAKISNIIGIK
                     EATGQMTRLQQILRLCEGSLDVYSGDDLTAAQWLLAGAKGVISVTANVAAKLMAKMCD
                     LAMDDDQAGCLRIQEQLMPLHELLFVESNPIPVKWAMNKMGLIGGELRLPMTELSEKH
                     HQALEKVLKNLELI"
     gene            15941..16195
                     /locus_tag="ACF2SP_001223"
     CDS             15941..16195
                     /locus_tag="ACF2SP_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HFD2359017.1"
                     /translation="MKKIIPTLFFIGISTTMMVGCTNTQVGTAVGAAAGAGIGYGVSG
                     GSALGTAIGAGAGALVGGAIGQEQDRREYYYYSGYPYYYY"
     gene            16473..17255
                     /locus_tag="ACF2SP_001224"
     CDS             16473..17255
                     /locus_tag="ACF2SP_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFD2359018.1"
                     /translation="MKLKNKVAIVTGAASGIGKEIALVYAKEGAKVAIADLNLDQASH
                     VAEEIKSSGGQAMAVAMNVTDEAQVNQGVDKVADHFGGVDVLVSNAGIQIIESVDKLA
                     FSDWKRMLAIHLDGAFLTTKACLKYMYASGNGGSIIYMGSVHSKEASVLKAPYVTAKH
                     GLLGLCKVVAKEGGTHKVRANVICPGFVRTPLVDKQIPEQAKELGISEEDVIKKVMLK
                     ETVDGEFTTTDDVAQTALFFASFESNALTGQSLVVSHGWFME"
     gene            17262..18422
                     /locus_tag="ACF2SP_001225"
     CDS             17262..18422
                     /locus_tag="ACF2SP_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2359019.1"
                     /translation="MTRTKKSARQIEYQHIACSFQGGGALGAYQAGVLHALNEVGYHP
                     HWYVGTSIGAINAAIAAGNSDKERIEKMYQFWESIATPSAVNESTLLDDKISRKIQHL
                     IAAHSALLLGQPGFFVPRFPSPDFGWHNNPDLLSYYDTSPLHNSLEKFIDFDRLNNNK
                     LTRISVGAVEVSSGMMTYFDSYKQKIGPDHIMASGALPPGFPAVKVDGKYYWDGGISS
                     NSPVNYILNHNNQKQMLCFMIHLFDSFGMIPTSMDEVLKRKKDIEFSSRFIKLINLHK
                     EIHDLRNTIHILSNHISPEEKAKPEIRECIKKGRPSAISLVRFLYEHDETELSSKDYE
                     FSKKSIHERIQKGYDDGKNAIKKSPWNQSVPMTEGIALYDMSAKDTMKKYQD"
     gene            18452..19357
                     /gene="motA"
                     /locus_tag="ACF2SP_001226"
     CDS             18452..19357
                     /gene="motA"
                     /locus_tag="ACF2SP_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFD2359020.1"
                     /translation="MLIIVGYIVILLCVFGGFSLTGGHLAAIFQPVELLIIGGAAIGS
                     FIVGNNITVLKSVLKALPATFRGQKSAKQASSDLLSLLYHLLSKARQQGLMSLENDID
                     EPDKSPIFTSYPNLMSNHHLIEFICDYLRLIITSNMQPFQLESLMDMEIESHHEEEMI
                     PANAITKLADAMPAFGIVAAVLGVVHTMESISLPPSELGVLIAHALVGTFLGILIGYG
                     FVGPIATAMEQNANQNQLMLQCIKVTILASLHNNPPIIATEFGRKVLFTSQRPSFNQL
                     NDEIKNAKTAQTTTQNSEATEATSS"
     gene            19367..20305
                     /gene="motB"
                     /locus_tag="ACF2SP_001227"
     CDS             19367..20305
                     /gene="motB"
                     /locus_tag="ACF2SP_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFD2359021.1"
                     /translation="MSEINDISDPINDDLEKKDKREKKEKTAQYTPIIRKIKKNDHKH
                     HGGSWKIAFADFVTAMMAFFLLMWLVASLNKAQKAGIAEYFKQPMKVALFGGESMGNR
                     KATIKGGGPNIEDTDGQVSATNKPLINESIAKDKEIIESQANEIKKLEELKSEINLSM
                     EKDPSLAGLKKQLLMDVVSDGLRIQLIDNQKKPMFDVGSDKINPEIEPILANIAKLLN
                     SVPNKITIQGHTDANPYHNPEELEYTNWELSAQRANAARRALIKAGMDENKVMTVTGY
                     SSTVLLDKKNPYNPENRRISIIVMKKGAENKIKVNK"
     gene            complement(20371..20808)
                     /locus_tag="ACF2SP_001228"
     CDS             complement(20371..20808)
                     /locus_tag="ACF2SP_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359022.1"
                     /translation="MKRIILFMIAWSLLNSAYSSHSLPSHASILFTLANTERSNSCPA
                     LLHNAKVVVDYDYNFERNMGLAHLRQLQSTKLSETLHPLGLSNYYAFMSDMKPKAVQI
                     EGGEVTIYRIIFHLYKNGDSRIYLMINQDGECIMSSDVVNVNL"
     gene            21089..22384
                     /locus_tag="ACF2SP_001229"
     CDS             21089..22384
                     /locus_tag="ACF2SP_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter"
                     /protein_id="HFD2359023.1"
                     /translation="MSSNESAAIVSQIVNQKLSQWKKQDTIWMLSLYGTAIGAGTLFL
                     PIDAGLNGILPLIIMTILAFPMTYYSHRALCRFVLSGSSSHNDITDVVEEHFGSFAGK
                     MLTILYFFAIYPILLMYSVAITNTTESFLINQMGITPPPRALLAIILILGLMGIVRLG
                     QEIIVKSMSVLVYPFATILFLLSLYLIPNWNNSIFIQSNSLHANGGHGLLMTLWLIIP
                     VMVFSFNHSPIISSFAVNQKQQYGSQADEKSSLIMKYSHLMMVFSVMFFVFSCVLSLS
                     PQDLLQAKQQNISILSYLANHFNTPIMAYIAPVIAFIAISKSFLGHYLGAKEGLSGII
                     VKSLKSTTGKTINKNSLHRIIEIFMILTCWVVATINPNILKMIETLGGPIIAVLLFIM
                     PMYAIAKVPAMKKYNNQISNVFVTVMGIISISAIIYGLI"
     gene            complement(22477..>24223)
                     /gene="ankK"
                     /locus_tag="ACF2SP_001230"
     CDS             complement(22477..>24223)
                     /gene="ankK"
                     /locus_tag="ACF2SP_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkK/LegA5"
                     /protein_id="HFD2359024.1"
                     /translation="QLARLFLDNGLTSFQKMVVNDANKVQGLIVEHLNYVIENKEGLK
                     QPFYTLNAPKNECDCTEKRVTNSNEIPFYFLDKLPQGFFNQLLAAEKNNKLSIDYASL
                     ASILATSYTLEEDDLHKGNFGFYLVKKQGKPRVVFFKIDHDLMFVDSIMSFTTRRFCH
                     LFDGCDAFDITEEDLLKFPNLKYSANGYWPTKTSFFYKPWDNKDYRTYAEIQAFADLS
                     HVEEFNKAKWRSFYKHILISQSQMEATLKACFDENNSSDRAHISLVIQAMLARQARLK
                     AMLFSLKDFRDFILSQNGKERDLLCHEILNNLPEEERKSFENEIRQSLDYNHNLCCSG
                     LFEDGDTPLHIAIKSGDYRYDETIGMYGQFINTKNSSGKTPLDIALQMAGQSKVHPAD
                     VRKDYCFIMKHLLANGANQTKQFEEFDKIENIRSYQFHTPYLNKAIKAKTYHELKEVL
                     RDIGEDHQYCLKFKKMLAVECISEFIKANQDNLSLRGILLKLKKEVDGKGTKSENAAL
                     MYIRQLRSRLWIVRQIRGLYGWSTTQGEIDYMIDKELARLDTKDLKRLSLFDSRDSST
                     LDNVFLDISLSKNKI"
BASE COUNT         7322 a         5132 c         4408 g         7361 t
ORIGIN      
        1 tcctttacgg gttaatcgtt taatacagta ttcttccaga taactgctca aaacataata
       61 atattcgtct aaaatcagaa tattaagaat tttctccgct ctttccagat gacgcttgtt
      121 agcgtgatca caagcctgaa acaaaatgcc tcccaagatt gcagcaatac ttgccttacg
      181 ttcaggactg gctaaataaa atcgttgtat gcattcatca agagccctgt ctttatccca
      241 ttcgacccac tcatcataca gtgataaggc cacatcaggt ttaaaatttt ctacgctggt
      301 cagctcctgc atagaatacg cgactggtac aacagcttgc acattagccc acgaacaatt
      361 tccactaatt tgagaactaa ttggcatttg agcaaaaggg agcagcccca attgttgatt
      421 aatcatttga tgataatatc gtctgttttg ttttttatat aaaaaatcat ggataaaatt
      481 gattgtcagt gcctctggcc tggttattcg gtaaatatta acgcttcctt cttgtaagct
      541 attttcaccc ctgtcaattt ttgcccacca ctggtgataa cggacaaagc ataaggcatg
      601 gccacgactc gccgcaggaa gaatcagcat aggggctttt aacaaagcag cgatacgttt
      661 gaaatgctgt tcgccaagtc tgggatgatg ttgatactgc aacagttctg ccgcatcagt
      721 aaaagcatcc ataatattat gaatatatgg aaaataatcc cttaaatgcc gagtcgaata
      781 actactgata aatctccgca gagagtcttt tacaacagca acagtgaatt ccaaaataaa
      841 cccttcataa tcaagctcga tgaattcacc tttcgcattg acaatatcca catcaccttg
      901 caattcaaat cgatgaccaa tcaatttggc attaataaaa tcaagagcaa aatcaagctt
      961 cgcaccatga tgatataaca actgctttat tgcatcttgg ccacgcaaaa ccgggtaaac
     1021 caacacacaa agaccattac gagtgtaggc attaggatca gcgccatagg ttaataagag
     1081 tttggtcata tccaaatcat tattgtctac tgcccaatgt aatggagtgc gtcctgtgac
     1141 gtctggcttg ttgatatcca cttttcgagc aattaactgc tctgctattt taatttgtcg
     1201 tgtaatggca cactctatga ggggagtaaa accatattca tcgatatcat ccaaagattc
     1261 ccctgcacgc agataagcat caaaatcagg cattctatga ctgataatat catttgcaat
     1321 actcatttag ggcccccggg gggtaggtgc tgttgtaaat tttggcatat tgcctaagcg
     1381 taattgtttt tctttatcct gttctcttct ctcattatca aactcccttc ttgcttctgt
     1441 attaagcggc ggttcagggt ttaaattagg atctattcca tcaaaacgct gactattcaa
     1501 ttcagggtgt tgcaatatat tgttttgcaa ctccccctct ggggcaacct tgatttcttc
     1561 ttcagggtgt tcacgcgctt taacagaagc aaaccttctc tctccctgct gttcagccaa
     1621 acgtcttcgt ctttccattg cttttgtttc agcaaccaag aaagtacttt cactgcgatt
     1681 ggaaggcaat cgtgtgggta actcaacatg aaatactgag cccaacaaga cataaacagc
     1741 tgcagaacca ccatccttgg gtttggcgct atgaaatgcc aatacctcat tgatctgagg
     1801 cagccaccga ttcaccagat ttttcattaa aggcggtttg tcctgagaat tatccgcgcc
     1861 atgaataatc agaacgcatt gtttgttatt ttttatttga gtttggataa attgatataa
     1921 gctcttacga gcagattcaa cgtttaaacc gtttaaatcc agttccgctt cccagggagt
     1981 ttttccattt tttaaatcat tgaagcgttt tgatgataaa ttgggtcgag agtaagacaa
     2041 tacagtactc gcgagcacta tttcttcgat ctcatcggat aaataatatt ctttcgccat
     2101 ctttttcaaa atttaacagt cataatagaa gtataactta ggatataaaa agtatggcat
     2161 tttttctaat ataacctgca tgaccttagg gggcatacag gttttctatc aagatactgg
     2221 acctatgcta ttgcgggggt gggagcttca cagaaactct ttttgcagta tttcagcaat
     2281 ttgtactgcg ttagttgctg cacctttgcg aatgttatcc gcgactatcc acaaatttag
     2341 tccacaagga tgagaaatat cctgtcttat gcgccctaca aaaacatcat catgcccaac
     2401 tgcattctta attgctgtgg gataacttgc tttagaaaga ttatccacta cagtaacgcc
     2461 gggtgccttt gccaaaagcg cgcgagcatc gtcagccgtc aaaggctttt ttaattccag
     2521 atgaaccgct tcagaatgcc cataaataac gggaaccctg acagctgtag ggttaaccat
     2581 aatgctgtca tcttccataa tcttgcgggt ttcccagacc atcttcatct cttcccgggt
     2641 ataaccattg tcttcgaact gatcaatatg aggaagcgca ttaaaagcaa tttgctgagg
     2701 atagacttga acattagctg gtctcccatt taaaagatcg cctacctgag caaccagttc
     2761 gctgatggct tttttgccag tcccggaaac agactggtaa gtcgccacat taatacgact
     2821 aataccaaca gcgtcataga ttggctttag agcaacgacc atctgaatgg tggagcaatt
     2881 agggttagca ataatgcctc ttttagtata atcagcgatt cgatgaggat tcacttcagg
     2941 gacaacaaga ggaatatcat cctcataacg aaaacaggag gtattatcaa ccaccacgca
     3001 cccagcagcc acagcttttg gcgcatactc ttttgaaaca gcgccgcctg cagagaataa
     3061 agcaagatcg actttgctaa aatcaaattc tgctaaatcc aaaacatcca attcctgatc
     3121 tctaaaagtc actgtcttac ctactgagcg agaactcgct aatgggtaaa gggacttaat
     3181 tggaaagttt ctttcttcca aaacggttaa aaaggtttct ccaacagcgc cggtggcacc
     3241 tacgatagct acattcaaat gtctgctcat tatgacctca tcttaccatc aattaacaat
     3301 gtgttagtca agtacaattc ctgcttgcgc agcaaaaaac ggactacgat atggctatat
     3361 tttgaaacgt tataatttat tatttacact gcgctttatg tcgtaaataa tgatccatta
     3421 acaccaaagc catcattgcc tctgcaattg gcacagccct tatccccacg caaggatcat
     3481 gccgcccttt ggttactaca gtaacctctt caccttcagt attaatggtc tggcctggag
     3541 ttgtgatgct tgaagttggc tttaaagcga tgctcacttc gatgggttgg cctgtcgcta
     3601 tgcctcctaa aataccaccg gcatgattac tcaagaatcc cttggcggtc atctgatctc
     3661 tatgataact tcctaactgc tccactgcat taaaaccagc tccaatttca acacctttaa
     3721 cagcattaat tgacatcatt gcataagcaa gagtagcgtc cagtttatca aatacagggt
     3781 ctcctaatcc tgttggcact ccccgtgcca gtatctttac ccgagcacca acggaatcac
     3841 cctggcgtct taaacgatct atataatcag ccaactcctg agtttgctta ttattaggac
     3901 aaaaaaaggg atttttattg atttcatttt cattttcaaa ctgtaacttc aaatcgccca
     3961 tttgttgcaa ataaccaata atatccaagt ttaaataacg ctttaaatac aatctggcaa
     4021 tagcaccggc agcgactcgt gcggctgtct cacgagcgga agaacggcct ccccctctat
     4081 aatctctatg cccatattta taatgatagg taaaatcagc atgccctgga cggaacaaat
     4141 ttttaatgtc ttcataatcc cgggaacgct gatcagtatt ctgtatcagc aatgcaatgg
     4201 gagctcctgt cgttttccca tcaaatacac cagaaagaat ctgtacctta tcttcctctc
     4261 gtctctgggt agtatattta gattgacccg gtttacgttt gtctaaaaaa ggttggatat
     4321 ctgcttcagt taaagacatg ccgggaggac aaccatcgac tacacatccg atagcaggac
     4381 cgtggctttc accaaaggtt gttacagtaa ataacgctcc aaaggtattc cctgacatgc
     4441 cttactcgct tatttaaaat agtcttctaa ttgctgtttt gttaataaaa acaccccttg
     4501 cccgccgttg ctcatttcaa gccaggtaaa aggaaccata ggataagtct cgcataaagc
     4561 ctcttcacta ttacctactt ccactaccaa aatcccatgt tcacttaaat aagcatgggc
     4621 attctttaaa attttttcaa taatagccaa gccattattt cctgtttcca atgccaaaac
     4681 cggttcatgg cggtattcat ccggcagggt ctgcatctct tccttcccca cataaggagg
     4741 attgctcacg atcaaatcat attgtttacc tgacagtgcc gtaaaacaat ccgattcaat
     4801 cagtgccaat tgatcgccta catcatgacg ttcacaattg atagtagcga cagccaatgc
     4861 ttgatttgaa atatccacag catccacctg agcctcaggg aatgcataac aacaggcaat
     4921 cgctatacaa ccactcccgg tacataaatc caaaacatga tgcactcgtt ctgcttctat
     4981 ccaaggggaa aattgattat tgattaactc tcctattggt gatctgggta taagcactcg
     5041 ttcgtcaaca taaaaaggta aatcgcaaaa ataggcttcc ttaattaaat aaggtacggg
     5101 taccctttga ttaattcgtt tatccaattg attacaaaga tgtttgcgtt cactcgttgt
     5161 taaacgtgca ttcaacaaaa aagagtcaac atcgtatggt aaagacaagc ttctcaatat
     5221 cagagatctc atgtcatccc aagcgttgtc agttccgtgg ccataaaata attgcgcgtt
     5281 ttctgcacaa gacaaactaa accgtaaaaa atccagaata gtaaccagtt cttctgtttc
     5341 cttgctaaaa taactactca caagcgtatc aaccataacc atagaaaaag tacaaattgt
     5401 acactaaatt aaaatctatg tcctgtattc tttgttaagg ggtgcgattt aagacgcccc
     5461 ttcaaatggc tcgattttca cctaacctaa agaattactt caaattaagt tataattaaa
     5521 tatattcttt tgatacctgt taaaaaatga ctgatgattt cctgtctgat gaggataaag
     5581 ctctatttcg tcatcatatg cgttctgtaa aaccattaaa cgaaaaaaca aagcgcgtaa
     5641 aaagccccac gcaaccaact catgaaaaaa aaaccacaac tttcaagagt cagccggtac
     5701 ccaaagaaga atatttttta tctgatttta ttcgtgacac tgttcaaccc gatactattt
     5761 tatctttttc agaaccaggc attcccaatc agagattccg tgcactaaaa aatggacaaa
     5821 ttccatggga ggccaagctg gatttacatg gattaaaagc tgaagcagct cgccaagcgc
     5881 tttatgattt tatcaagact cagtcactca ataataagcg ctgcttactt attgttcacg
     5941 gcaaaggagg ccatcaaggc gctcccccgg taataaaaaa cctaatcaat cgctggctgc
     6001 cgcaatttag tcaagtcctg gcattccaca gcgctcttcc aaaggatgga ggattgggag
     6061 ctgtgtacgt cttattaaaa agaaacagag aaaaggatga acagggcaat tactagtaac
     6121 actttacttg cgttaaagca gtaatcttag gataattcgt caaattaaaa aatatcttga
     6181 gtacagctat ggaaaaatta ggtcagttca tcatcaatca ttggcaactc tggctcgcct
     6241 ttgttgtggt tgtattttta attttcatta atgaaatact ctctcaaaga aagaaagcca
     6301 aagaagtttc accgcaatcc ctggttgatt taatgaacaa tggaaatgcc atcgtaatcg
     6361 acatacgcga caaagaatca ttcaaaaatg gccatatcat taattcaatg aacgtaaatc
     6421 ctgatgattt tgaacaacaa aaaatgagca agtacaaaga taaacccatt atcctggtct
     6481 gcgcacgagg cttacaatct ccaaccctag ccatgaaaat ccgtaatcaa ggttaccaac
     6541 ccctggtttt gagtggagga attcaagcct ggcaaaatgc tgatcttcct ttagtgaagg
     6601 gtaaatgaac agatgaatga agtcatactc tacactacag gctattgtcc ttattgcatc
     6661 aaagcaaaag agctactaga tagaaaaaag gttatctata ccgaaatccg cgtcgattta
     6721 cagcctgaat tacgtgaaga aatgatacaa aaaagtggcc ggcgtacagt gcctcaaatt
     6781 ttcattaatg gccaggctat tggtggctgc gatgatttat atgccctgga agctcaggga
     6841 acattaaatg aacttttaaa aaaataggaa taaaacatga ctgaacaact gaataccaat
     6901 cagcaaaatg atgaagctca attcatgatt caacgaatct atatcaaaga tttatcctat
     6961 gaaacaccaa atacaccggc tgtttttcaa caacaatggg aaccagagct caaactggat
     7021 ctcaatacca cgactactca actggataaa aacgtttatg aagtggtttt aactgttaca
     7081 accactgtga tgaatcaaaa aaccacagca tttttggttg aagtaaagca agcgggcatc
     7141 tttactatcc aaggcgccgc agcaagtcag ttggatcact tgcttcatag cttctgcccc
     7201 agcatcctgt tcccctatgc tcgtgaagca attacctcgc aagtcattcg tggcagcttc
     7261 ccccaattag tactcgcacc gattaatttt gatgctttat atatgcagca gctggaagaa
     7321 aaacaaaaag caactggagc taaagacgag actgtttctc actaagatga ataaaaaaac
     7381 cattgccatg ttaggtgccg gctcctgggg cactgctgtc gcaattcatc tagctaaaat
     7441 agggcataaa acgctgctgt ggagccataa tccacagcat gttgccctca tggctgaaca
     7501 gcattcaaat ccagcttacc ttcctggtat accttttcca gagaatttga ttccttctga
     7561 tgatttaatt gaatgcgttc aattagcaga ttatgtcatc atagcagtcc cttctcatgc
     7621 ctttgcggaa ataatcaata aaatccctaa accaacccaa ggattggcgt ggcttacgaa
     7681 gggtgttgat ccagcgagtc atcaattatt aagccaactg gttgccagtc gatttggggt
     7741 tgattttccc atagcagtta tttcaggccc atcctttgcc aaagaagtgg ctcgcttcct
     7801 gcctacagct ctcactttgg ccagtaacaa tacgaactat caaaaaaaaa tgcatcaact
     7861 atttcatcac gacaacattc gtgtctattt aagtgatgat ttaataggtg tgcaattatg
     7921 tggcgctgtt aaaaatatcc tggcgatagc ctgtggaatc agtgatggct tagggtatgg
     7981 cgctaatgcc aaggcagcgc ttattacccg tggacttgct gaaatgactc gtttgggatt
     8041 aagcatggga gcacggcaag atacttttct aggcttggct ggtgtagggg acttggtatt
     8101 gacttgtact gatgatcaat cgcgcaatcg ccgctttgga ttgctcttgg gccgtgaagt
     8161 gcctattcct gaagccgaac accaaattgg ccaagtggtt gaagggaaac acaatgcagc
     8221 gcaaatttgc gctatagcca ataaaaataa agtggaaatg ccaatttgtg agcaaattaa
     8281 tgcgctattg catggtattg ttcatgccca acaggccgtg aacaacttaa tgagtcgccc
     8341 cgccaaggaa gaataacctt tgcccttgta aaaaatcata ttaaattcaa tttagtgcac
     8401 caggacgcag atgatacaga tggatagacg acactaaccc atagcatcaa tcaattcaat
     8461 atcactcaaa ctcaattgct cccctaaaaa tatctcgcct accttctgga acctattgat
     8521 agaatctgtt accaaatagt tgattctggg gggaacttcc aaattgtcat tgtgcagatt
     8581 ttgattttgc aataattcat aaagagaaat tgcagtcgcc tgagcagaat cgacaattgc
     8641 aacatctttt ggcaataagc tggataacaa gggtttaaat actggaaaat gagtgcaccc
     8701 caataacaca gcatcttcat ccgaaaaatc gtcgagataa tgcttcaatg cctcacgagc
     8761 aactgcatta gtcaccatac cttcctctgc caaggcgact aaaacactac atgcccgact
     8821 gctgacaacg gcctcaggca aattctttat gatcaaatca tgataggctc tggaagcaat
     8881 ggttgtttct gtagccagaa cagcaatacg atgatttttg gtagctgaca ccgcagcggc
     8941 tgcaccaggt gccaccacac ccagaaccgg aatatcggtt aacatgctct gtaaatgggg
     9001 taaggcagcc gtcgttgcag tattgcaggc aatgactaga gcttttatgc gacgctcaac
     9061 cagaattttt gccatttgca tagcgtattg cttgacagta tcgggacttt tcgtaccata
     9121 aggtaaacgg gctgtatctc ccaaataaac aaaagattca tgaggtaatt tatcccgcag
     9181 agcccttaaa accgtaagcc cccccatgcc tgaatcgaat acaccaatag ccaaatcctt
     9241 gttagagttc aattgattac tccttcttgt catttatttc ttggctctct tatttggcaa
     9301 acgcttcgtt ctaccaaaaa tgagagccaa agtcattatt atttacttac gcatccctgt
     9361 actcggactt tcaggcgctg gtccttcttg ttcaatggtt ctttcagttg ttttatgcac
     9421 cggattcagt tccttcttca tcttgctttg tttatcagtg atctcttgta ctttggaatc
     9481 cactttttct ctttctttct gaggactaaa tttctcatca gtcatttgct gcatatgttt
     9541 tattttagac tgtatgatat tctgggtctc tgttaatcct gtgttagtaa atacctgact
     9601 atacaatgaa ttgctgctaa atccccacaa ccgtcctttc acattatccg gatggaatac
     9661 tgcagaattc acctcaatcg cgtcacggtt aaacttgaat tttggattct tttcaccaag
     9721 gatcaccagg gcagtgtaca aatattccag ttcttcggga ttagatccgc gtaatcgtat
     9781 cggtttatcc ttggttggtg gactatccag actcgctaaa atatcagccg ccatcgccat
     9841 ggaaaaattg actttagctt ctaccagagg atctccactt ggagggtttg gaggtggaac
     9901 ttgggtcggg aattgtaaaa tctcaaattt acttccggga accttgctta tttctccttt
     9961 tttgctcgtt acttgatcag gaacattgtt atcctgagta taccgtccta tcgtttccac
    10021 aggagcgccg gatttcggtg ctgttttatg agatacatca acgacaacaa tctgcccttc
    10081 cggtattttt tcaccaacaa gaaattcttg tatccttccg ttagacccag tagtagcggt
    10141 tgtactcgat ctcagagaag gattaggaac attgggtaat ggcggtatct gatcacgtga
    10201 aacaggagga tggcgtttga taccctcaga attgtagtag taatgcatct tccctttggc
    10261 tgccacgtcc acttctttta caatggtttc taactttccc tgtatttttt tataaaaatc
    10321 caaatccttg cgcaccgcat ccaactcagt ttgcaactca gtacgcaatt ttctgatttc
    10381 ctctttttgt tccagggtca agccagctgc cttaaattga ctatcttgag gcaagctctt
    10441 caaatggcct tcaagagcaa tcacttgacg tcgcaattga tcaacaacca gttcgcaatc
    10501 cctggataac ccttcatacc tttctttcat ctgagctgct ttcgatctgg cggtgccctg
    10561 aaaggttgga ttgtataaat gaactggatc aatatcattg ataaacttta atttatctgc
    10621 gtttgttctt attggtcctg tgattccctt aatagattct aattcagtac tcagagcttt
    10681 gattgctgtc atcacattag caggattagt atctaacaat tcctgtttcc tgagatcatt
    10741 ttttatctca ttaaataaag caggggttag ctctcttaaa caatcttgtt taagcttttt
    10801 aagtcccgga tcagtcggat ttttgccaaa taaattatca gagtattctt caacggtttt
    10861 actgttcttt agattttcca aaaagtaatt tgttataggt gagcttgtag gtgttgatgg
    10921 ctttcctcgt attccaaaaa ttacaagttt atccaaactt aataagacac ctaatagttt
    10981 tttatttcct gagttagcag gattattgta ttcaatatac aaattcttat tatattcttg
    11041 ctgcttactc atctcatctt ttcgaggagt tgagctggta aaagcactgc catcatcgtt
    11101 taaattaaac gcgttataaa aatctttttg atttacagag ccagattgag ttttaatagc
    11161 atcaatcaag gttttgtagt cggtgacctg ggtataggta ttggggttag aattggcggt
    11221 ggttaatgcc tgattaatag cagcaacttg cttatcattt aaagtgattt caggtttaaa
    11281 attagccaaa accctggcta cctcggcatt gtgaatagcc ctaaatccct cgagtctttt
    11341 attctctgtc aagagctctg caatgccgga tgcgtttttt ccaaggtaat gttctgctac
    11401 atgagattca taagcgttca ttaaatggcg taattgttgt ggcttggcca aaatctctct
    11461 ttgtttttcg actggaagct gggttaatgt gctcattaat ttcgaatggg ccccgctgcc
    11521 taaagaagac actctattta tttcaagttc aaactggcga tctcttgccc attgcttcat
    11581 atctttcaaa tcggtgtctt tcacccaatc cgctggagta atacctaact tcccgagtac
    11641 ctctttaaat ttatccaaat ttttagcccc gtctaatgct tttaaagcat ccagggcctt
    11701 taaatttgtt tcatccttaa tattattaat aaagctcttc atcatatttt ttttgagcga
    11761 gcctaaattt tcttccgtca ccgttttatc aatgtagtca tggggtccca aaagagcagg
    11821 ttttcttaat tcagcaatca atgcattggc atctttagca ttgagagcat ccttgagatt
    11881 ttgagttcct gatgataaaa cggcttgaag ataacgggct cccaatactc cttgggctct
    11941 ctgccattct gattccggta aatacgtctt gtagggttca tccaaactgt ttttaaaccc
    12001 tacagctgtc gtactttcta acccgttttt tttggctaat agcgcatcgt gagataatga
    12061 ttggacataa ctatcaaatc ctgccttggc agcttcttct tccagctcct gaattatgtc
    12121 atccaaagct tcttctactt catcacttaa gggataagat aattctccat ttccctcggt
    12181 tggaaaacct atggcatttg cggcatgctc tgcttcagtt ttactattag ctgttagcaa
    12241 atctttcaat aattgccggt tttcacagtt tttaatcagt ttaagcaata ataattcgct
    12301 tgcctgttct ttaatctttt tgattgcccc atcagataaa acgtcagtgg atttgttaat
    12361 ggctttcgga ggtattgtcg gggtatattc ttcttttaac caaccatgca cattcccttc
    12421 actaaaattg cccaaaccag gttttttgga ttcgatatac gctctgcact cttcagggtt
    12481 gttttttaaa atagcaacca aagtatcagt atctgatttt tccaagtcaa gcttgactcg
    12541 ttgttcagca gcgaattgat gtaatttttg aaaactgttt attgggtcat tagaatcgag
    12601 gaaatcatct gattctaaat attcttctgt gcttggtcca aaaaataatc cttcattaaa
    12661 ctccatcaag ctgggatcat gctcatccca gaagtcctga tgctcagcta ttatctttct
    12721 aaaagcgtca ggatcagaag gatcagtttt cagtatatca tctaaaactt ttgcaagctc
    12781 atcacgaaga gtttcatcat ttttcgctga taccgcaatt tcttccagcg catcaaaaaa
    12841 ttctttattt tttgccatac ggatgtactc ctcgagaatc attatgtgtt cattttaagc
    12901 caaaaaactt aagttatcct taaattttgc cgatattaat accacagatg aaaacctaag
    12961 ctagctttat aattagaagt atagcaagga taaggtgaat agaatgagaa taaaaataat
    13021 tctttgtggg ttaattctaa caaacctcac ctatgcccaa ggctgtattg ttggtggtca
    13081 ggcctctcaa cccaggtatg tctgttttga tgggcgaacg ttggatctga ctgaaacagg
    13141 ccttgtatgg aatgccaaac ggggatgttt tatcagctca gtaccttgct gtaactacaa
    13201 tgccactcat tatgcctatc acccaaatcc ggccaaaata ggcggagcct atgcccgttg
    13261 caggcacgac tatccgttta ctcttggcca aatgcaaact cattaatgtt taaaccaaca
    13321 aaaatcgaaa attcttttga atttcattta aatcaaactg gttcaaatac agagagaaac
    13381 tcatccacgc acttaatgca gccaaatcct ggaaaggagg tggaagatac actggatccg
    13441 ttaaaatctc gcgttctttt aattcaccta gcaaaggtaa atcatccaaa accagcttgc
    13501 cggtaaataa tagtgggatt gtgtcgactc ttttctgccc tatagcaaag cgtatgtaat
    13561 tgtaaattaa tacaaatccc ttggcatagg ataaatcctt ggtaaatggg ccacctgtcg
    13621 gtgtgctgcc tctaaatata cgtacggact ggttatagct gtcttcttct gacaatccgc
    13681 actccaggaa atagcgataa atatcaataa aatctgcccc ttgagtcacc ttatccaggg
    13741 caatcacccg gtttgtaatt ttcaacatcc gacctggata agaagaaaat gtcacgactt
    13801 cagtaatgac tgccaagccc tcctgaatca cgctgctgga aggagaacct ttagacagga
    13861 aaaagcaatt cggttgcaag gctccattga gagtcgttcc cacatgaacc cagccctcat
    13921 gaacttccaa atagcgtaaa tctctatcgc taaacatagc gttctgactt aacttgatgc
    13981 tgtcagctcc agcagaagca tcagcaatca tatcatcgct gaccataaca ctcactcggc
    14041 caggatgctt gtcaaaaaat ttctctaatc gctcctgtaa aatttcctga gcctgtatcg
    14101 gagtgtaacg cttgacatca gcctgagact gcaattggac ctggagattc tttaatacat
    14161 caaatagcag ggaacccatt tcacacatac gcggtccacc cacataaaac acatcgtccg
    14221 ggctaccata caattccata gccatttcgc taaatgaagg agtaccccgc aaggataaca
    14281 tctgggctgc gcggctatac tcttcgcatt gacgtctgat taggcgagtt actgtggagt
    14341 attgccctaa ttgattctgt gcgtcacgca aaatgatacg aaactcttcc tgcttgtctt
    14401 tgactttaaa aggcagtggc ttaccgtcat aataagcctt atcaatagaa ggaagttttt
    14461 ttcccttgaa cttaaaaaat tcctgtttta ctgattcatc ccatttgatg ctatcaagaa
    14521 tcctgatttg acgctgcgcc tccacgatac gctgagacaa ctcctgtatt attgacaact
    14581 ctcctgattc tgatggcgac atgatgaatc cttatgaagt tgtgatatct tccaccggcg
    14641 gcgctatact cacccgcgca tcctgagttt gttgaggtaa atcataaaaa ttactaatat
    14701 tggcactagt caatggaggt ggtactacca aagcttcgcc atttcggctc tgtaaatata
    14761 aattttctcc attactggca taacgagaat tacatgctgt tagtgccagt gctgccaaga
    14821 ctataaaact caattttttc acaacaaaac cctcagttat atcaactcta aattcttcaa
    14881 taccttttcc aaagcctgat gatgtttttc agacaactcc gtcatcggta atcttaattc
    14941 ccctccaatt aatcccattt tgttcatagc ccatttcact ggaattggat tcgactccac
    15001 gaacaaaagt tcatgtaaag gcattaattg ctcttgaata cgcaaacatc ctgcttgatc
    15061 atcatccatt gccaaatcac acatttttgc catcaattta gcagccacat tagccgtgac
    15121 tgaaataaca cctttagccc cggccaacaa ccattgggca gctgtcaaat catcgccact
    15181 gtaaacatca aggctccctt cgcataaccg caaaatctgc tgtaaacgag tcatctgtcc
    15241 ggttgcttcc tttattccta ttatattcga aattttagcc agtcttgcta ccgtttcggg
    15301 taagatatca caggctgttc ttcccggaac attatataaa ataatcggaa tcgctacgga
    15361 ttgggcaata tgactgtagt gcaaatacaa tccttcttga gtaggcttga tataggctgg
    15421 tgtcatgatt aaagcagcat gcgccccata ttccatagct tgctgggtta gttcaataca
    15481 atccttggtc gcattcatag cagtacctgc aatcacaggc actctttctt ttgcttgttc
    15541 gatgactgtt ttgatgacca aaagtttttc gctatgtgat aaagtaccag cctcaccagt
    15601 cgtgccagct gcgaccaagg catgagttcc cttagctata tgaaattcaa ccagttctcg
    15661 caaatgatga acatcgacgc tatcatttcg catgggggtt accaaggcta ctatacttcc
    15721 actgaacatg ttgccctcaa aaattaatga tccatgatcg tatattactg gaaaacagac
    15781 acccgagtaa atttttatat aacactttag tcaactggaa tctctatcag caatcattct
    15841 cttcaatccc aaacacatat ttgtttaaat tttttacata tttagcttat tttgtactat
    15901 tcttgatatg aaggcaacaa cttacaaaag gaagtttact atgaaaaaga ttataccaac
    15961 gttgtttttc atagggatat caaccactat gatggtaggt tgcacaaata ctcaagttgg
    16021 aacagcagtt ggagcagctg cgggcgctgg tattggctat ggagtttctg gaggatcagc
    16081 attgggaact gcaattggag caggagctgg tgcattggta ggcggtgcca tcggtcaaga
    16141 gcaagacaga cgtgagtatt attactacag tggctacccc tattattatt attaattctg
    16201 actagtcaac taaatatatt ccttaatcat ttttagcgat cataagagcc tggtcttttc
    16261 tagatagaga aaaggccagg atagttattg atttgggtaa aaccaaaact cttggcgaaa
    16321 tgatacagaa aagaagcaat tacctccctt aaagacaact ctctaaaaaa cttaaacaat
    16381 tcatcaaaga tttttgttag caaataataa ggcattatta aaaatccatt gctataatca
    16441 attcatggaa aacaatgaca aggatagtaa agatgaaact gaagaataaa gtcgctattg
    16501 tcacaggagc agcaagcgga attggcaaag aaattgcgct tgtttatgct aaagaagggg
    16561 caaaggtagc aatcgctgat ctgaatcttg atcaggcaag ccatgtagca gaggaaatca
    16621 aatcatcagg cggacaagca atggctgttg ccatgaatgt aactgatgaa gcacaagtca
    16681 atcagggcgt ggataaagta gctgatcact ttggaggagt tgatgtttta gtcagcaatg
    16741 ccggtattca aatcatcgaa tcagtggata aattagcctt ttcagactgg aaaagaatgc
    16801 ttgccattca tttggatggt gcctttttaa ctactaaagc ttgcttgaaa tacatgtatg
    16861 cctcaggcaa tggcggcagt attatttata tgggctcggt ccattccaaa gaagcctctg
    16921 tactcaaagc accttatgtt actgccaaac atggtctatt gggtttatgc aaagtagttg
    16981 ccaaggaagg tggaactcat aaggtaagag ccaatgtcat ttgcccggga tttgtccgta
    17041 ctcctctcgt tgacaaacaa attccagagc aagcaaaaga attaggcatc agtgaagaag
    17101 atgtcatcaa gaaagtgatg ctaaaagaaa cagttgacgg ggaattcacc accacagacg
    17161 atgtagcaca aaccgcttta ttttttgcat catttgaatc gaatgcgctt acaggacaat
    17221 cattggtagt cagtcatggt tggttcatgg agtaaacaac catgactcga actaaaaaat
    17281 ctgcaagaca gattgagtat caacatattg catgcagctt tcagggagga ggggctttgg
    17341 gtgcctatca agctggagta ttgcatgctt taaatgaagt gggataccac cctcattggt
    17401 acgtgggaac ctcaattgga gcaattaatg cagccattgc tgcaggtaac tctgacaaag
    17461 aacggataga aaaaatgtat caattttggg agtctatcgc tactccgtcc gcagtcaatg
    17521 aatcaactct cctcgatgat aagataagtc ggaaaattca acatttaatt gctgctcact
    17581 ccgctttact cttgggacag ccaggttttt ttgtacctcg tttcccttcc cctgattttg
    17641 gttggcataa caaccctgac ttattaagtt actatgatac ttctcctttg cataacagtt
    17701 tggaaaaatt tattgatttt gatcgtctga ataacaataa attaacccga atcagtgtag
    17761 gagccgttga agtaagtagt ggaatgatga cttattttga ttcctataaa caaaaaatag
    17821 gacctgatca cattatggca agcggtgcgc ttcctcccgg ctttccggca gttaaagttg
    17881 atggtaaata ttactgggat ggcggcatat caagtaattc accagtcaat tatatcctga
    17941 atcataataa tcaaaagcaa atgctttgtt ttatgattca tttgtttgat tcttttggga
    18001 tgattcccac ctcaatggat gaggtattaa aacgtaaaaa agacattgaa ttttccagtc
    18061 ggtttataaa attaattaac ctccacaagg aaattcatga tttaagaaac acaattcaca
    18121 ttttatcaaa ccatatttct cctgaggaaa aagcaaaacc tgaaataagg gaatgtatta
    18181 aaaagggacg tccttccgct atttcactgg ttcgattttt atatgaacac gatgaaacgg
    18241 aattgtcttc aaaagattat gaattctcta aaaaatctat tcatgaacgc attcaaaaag
    18301 gttatgatga tggtaaaaat gcgatcaaaa aatcgccttg gaatcaatca gtcccaatga
    18361 ctgaaggtat agcactgtat gacatgtctg ccaaagacac tatgaaaaaa tatcaagact
    18421 aattggacac ctgatactat aggataggga tatgttaatt atagtaggtt atattgtaat
    18481 actactctgt gtatttggtg gattcagcct gacaggcggc catctcgccg ctatatttca
    18541 acccgttgaa ttattgataa tcggaggggc cgccataggt tcctttatcg ttggtaataa
    18601 tataactgtt ttaaaatctg ttctaaaagc tttgcctgct acatttcgtg gccaaaaatc
    18661 ggcaaagcaa gcctccagtg atttgttaag ccttctatac catttactca gtaaagcaag
    18721 gcagcaaggg ttaatgtcgc tcgaaaatga cattgacgag ccggataaaa gcccaatttt
    18781 taccagttat cccaatttaa tgagtaatca tcatttaata gagtttattt gtgattacct
    18841 gagattgatt attacttcca acatgcagcc ttttcaattg gaaagcttga tggatatgga
    18901 gatcgaatct caccatgaag aagaaatgat cccggccaat gccatcacca aactggctga
    18961 tgccatgcct gcttttggta tcgtcgcagc agtattaggt gttgtccata ccatggaatc
    19021 catcagttta cctccctctg aattaggtgt tttaatcgct cacgcgttag tgggaacctt
    19081 tttaggtatt ttaattggct atggttttgt tgggcctatc gctactgcta tggagcaaaa
    19141 tgccaatcaa aatcaattga tgctgcaatg tataaaagtt accatcctgg caagtttgca
    19201 caataaccct cctattattg ctactgaatt tggacgtaag gtattattta catcgcaaag
    19261 accttcattt aatcaattaa atgatgaaat aaaaaatgcc aaaaccgctc agacaacaac
    19321 tcaaaactct gaagcaacag aagctacgtc cagttaggtt acagccatga gcgaaataaa
    19381 cgacatcagc gatccgatca acgacgatct ggaaaaaaag gacaagcggg agaaaaaaga
    19441 aaaaaccgct cagtataccc ctatcatcag aaaaattaaa aaaaatgatc ataagcatca
    19501 tggaggctct tggaaaattg cctttgctga ctttgtaacg gccatgatgg ccttttttct
    19561 actcatgtgg ttagttgctt cattaaacaa agctcaaaaa gcgggtattg ccgagtattt
    19621 taaacagcca atgaaagtag cattgtttgg cggggaaagc atggggaata gaaaagcaac
    19681 aattaaagga ggaggcccta atattgaaga cacagatggc caggtttctg ccaccaataa
    19741 acctttaatc aacgaaagca tagcaaaaga taaagaaatt attgaatctc aagctaatga
    19801 aataaaaaaa cttgaagagc tgaaatcaga gatcaattta agtatggaaa aagatccatc
    19861 gcttgctggt ctaaaaaaac aactcctgat ggatgttgtg tccgatggct tgcgcattca
    19921 actgatagat aatcagaaaa aacccatgtt tgatgtaggc tctgataaaa tcaatccgga
    19981 aattgaaccc atactcgcta acatcgcaaa actactcaat tcagtgccta ataaaatcac
    20041 tattcaaggc cacaccgatg ccaatcctta tcataatcct gaagaattgg aatacaccaa
    20101 ttgggaatta tctgcacaaa gagcgaatgc agcaagacgc gcattgataa aagctggaat
    20161 ggacgaaaat aaagttatga cagtcacagg ttattcttcc actgttcttc tcgataaaaa
    20221 aaatccctac aatcctgaaa atcggcgcat tagcatcatt gtcatgaaaa aaggggcaga
    20281 aaacaaaatc aaggtcaata aataatttgg cattcgacca aggctagccc cgtttcttag
    20341 gggttttcat taaaattaat actatttact ttacaaatta acattcacaa catcagaact
    20401 cataatgcat tcgccatctt gattaatcat aagataaatc ctgctatcgc catttttata
    20461 caaatgaaaa ataattcgat aaatggttac ctcaccccct tcaatctgaa ccgctttggg
    20521 cttcatatcc gacataaatg cataataatt acttaaaccg agcggatgta gtgtttcgct
    20581 caacttggtc gattgcaact gtcttaagtg agctagcccc atatttcgct caaagttata
    20641 gtcataatca acaacgacct ttgcattatg cagcaaagcc gggcatgaat tactacgctc
    20701 tgtattggca agggtaaaga gaatgctggc atgggatgga agtgagtgag aagaataggc
    20761 tgaattcaat aaactccaag cgatcataaa caaaatgatt cgtttcataa ctactccatt
    20821 aattaaacct gcttcgagaa acattcttat tacaatacga attcatttgc aacaatttaa
    20881 aagaataaga tcggaatgag cttcatgaat tgttaagaag gcaaggccat tttcctcgct
    20941 ccttacctta aattaatctc ttgctttgtt catttcagtt ggatatgatt tagttcgcaa
    21001 ttgttgaaat attgtctttg tagtgttaat attgatcaat ttgcgaggcg ctattttatt
    21061 agttagtatt aaaacgagga tgtttttcat gtcatcaaat gaatctgcag ctatagtatc
    21121 tcagatagtc aaccaaaaac tatcacaatg gaaaaaacaa gatacgattt ggatgttaag
    21181 cctctatggc acagcaattg gtgccggaac tcttttcctt cctatagatg ctggtcttaa
    21241 tggcattttg cctttaatca ttatgacaat tctggccttc cccatgactt attattctca
    21301 tagggcttta tgtcgttttg tcctttcagg ctcgtcctca cacaatgaca ttaccgatgt
    21361 tgtagaagaa cactttggct catttgcggg taagatgctg actatcctgt acttttttgc
    21421 gatttatcca attttgctta tgtacagtgt cgcaataaca aataccacgg aaagtttcct
    21481 gattaatcaa atgggaatta cccctccgcc acgagctctt ttagcaatca tattaattct
    21541 tgggttaatg ggtatcgtgc gtttaggaca agaaattatt gtcaaatcga tgtcggtatt
    21601 ggtatatcct tttgcaacta ttctatttct gctttcgctt tatttaattc caaattggaa
    21661 taactctatt tttatccaaa gcaattcctt acatgccaat ggagggcatg gactattaat
    21721 gactttatgg ttaattattc cagtgatggt gttctccttt aaccattctc ctatcatttc
    21781 atcttttgct gtgaatcaaa agcagcaata tggtagccaa gctgacgaga aaagctcatt
    21841 aattatgaaa tacagtcact tgatgatggt tttctctgtt atgttttttg tatttagctg
    21901 tgtgttgagc ctgtcacctc aagatctttt gcaagcaaag caacaaaata tttccatttt
    21961 gtcttatctg gcgaatcatt ttaatacccc tattatggcc tatattgcac cggttattgc
    22021 attcatagcc atttcaaaat cttttctggg acattattta ggtgccaaag aaggtttaag
    22081 tggaattatt gttaaatcat taaaatcaac aacaggcaaa acaattaata agaactccct
    22141 gcacagaatc attgaaattt ttatgattct gacttgttgg gttgttgcta caattaaccc
    22201 taatatttta aaaatgattg aaactttagg aggccccatt atagctgtct tattatttat
    22261 catgccaatg tatgccattg ctaaagtgcc agccatgaaa aaatacaaca atcaaatcag
    22321 taacgtattt gttaccgtca tgggcattat ttctatctca gccatcattt atggcttaat
    22381 ttaacaatca atctggtagt tgcatatatt tattaaggcg tatattacgc cttaataata
    22441 ttgctaaaca ttggtatttt tctcaataga atgaacttag attttattct ttgatagtga
    22501 tatatccaaa aagacattat ccaaagtact gctgtctctg gaatcaaata atgaaagtct
    22561 ctttaaatct ttagtatcca aacgcgccag ctctttatca atcatataat caatttcgcc
    22621 ttgcgttgta gaccagccat aaagtcctct gatttgacgt acaatccata gccgacttct
    22681 taattgacgt atatacatta aagccgcatt ttctgatttt gttcccttac catccacttc
    22741 cttttttaat tttaacagta ttcctcttaa actgagatta tcttgattgg cttttataaa
    22801 ttcagaaatg cattcaactg ccaacatctt tttgaatttc aaacaatact gatgatcttc
    22861 accaatgtcc ctgagtactt ctttcagttc atgataagtt ttagccttta tggctttgtt
    22921 tagataagga gtatgaaatt gataggatct gatgttttca attttatcga attcttcaaa
    22981 ctgttttgtc tgattagctc cattcgccaa taaatgtttc atgataaaac aataatcttt
    23041 acgcacatcc gcaggatgaa ctttagattg ccctgccatc tgcaaagcaa tatccaaagg
    23101 agtttttcct gagctatttt tcgtgttgat gaattgccca tacatgccaa tcgtttcgtc
    23161 ataacgataa tcgcctgact tgattgctat atgcagtggg gtatccccat cctcaaaaag
    23221 accggaacaa cagaggttat ggttataatc taacgattgt ctaatttcat tttcaaaaga
    23281 ttttctctct tcttctggca agttattcag gatttcatga cataataaat ctcgctcttt
    23341 tccattttgg gaaagaataa aatcacgaaa atcttttaga gaaaatagca tcgcctttaa
    23401 acgcgcctgt ctggctaaca tcgcttgaat taccagagaa atatgtgccc tgtctgaaga
    23461 gttattctca tcaaagcagg cctttaaggt agcctccatt tgagattgag agattaaaat
    23521 atgcttgtaa aatgaccgcc attttgcctt gttaaactct tccacatggg ataaatccgc
    23581 aaaagcctga atttcagcat aagtacgata atctttatta tcccatggtt tataaaaaaa
    23641 gctggttttg gttggccaat aaccattagc agaatatttt aaattaggaa attttaacaa
    23701 atcttcttct gtgatatcaa aagcatcaca tccatcaaac aaatggcaga atcttcttgt
    23761 tgtaaatgac atgatgctgt ctacaaacat taaatcatga tcaattttga aaaaaacaac
    23821 tcgcggtttt ccctgtttct tcaccaaata aaaaccaaaa ttccctttat gcaaatcgtc
    23881 ttcttccaaa gtgtacgaag tggccaatat acttgccaaa gaggcataat ctatgctcaa
    23941 tttattattt ttttctgccg ccagtaattg attaaaaaaa ccttgaggca acttatccag
    24001 aaaatagaag ggtatctcat tcgagttggt cactcgcttt tcagtacagt cacattcatt
    24061 ctttggagca ttcaaagtat aaaacggctg cttcaatccc tctttatttt caatcacata
    24121 atttaaatgc tcaactatca atccctgtac tttattcgca tcgttcacta ccattttttg
    24181 aaaggaagtt aatccattat ctaaaaataa tcgggctaac tga
//
LOCUS       DAXLDT010000040        24202 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXLDT010000040 DAXLDT010000000
VERSION     DAXLDT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24202
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.040"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..187)
                     /locus_tag="ACF2SP_001231"
     CDS             complement(<1..187)
                     /locus_tag="ACF2SP_001231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359025.1"
                     /translation="MSNFFDSSQFNEDENALIAKGIKLELVVALHEAVNASIAELGNN
                     LPKLTTRVKGSIKVILEL"
     gene            370..7383
                     /locus_tag="ACF2SP_001232"
     CDS             370..7383
                     /locus_tag="ACF2SP_001232"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2359026.1"
                     /translation="MPKNSNTTNLSQEDIDKVRESLENQWDSLGLNLFSKSKIFKTAK
                     KISKGIKTVDTHVKGEKQYYWCGVAGGTIDAGLNTLGSLAIGAMTGANPYALMLSQPA
                     ISKSAKAWGDLGQRGCDAAVDFFINRSRYQSANTYNSRFIGLSGTNSFHASSSFSSSS
                     NNSPSFNSYQVERTVKDAIRGEMSRLKPSMDSAYLRAHMNLGLGYNLSRSEFRSLASS
                     TNLISSYNLFNPSSKLSDVKAFAREIGGVAGEVATITDLIDSDAQAEADNYIFCFESD
                     QFPFSKEMVYQIQQELKEAYFTHKTSPFFSLHFNNFGYLYPVIHPALQNTLVGQIIAL
                     LDYWMKGFLNGGIYDEEFLKLWHEIRQCDESFLRQKIIDLKKYCETNADGLPYISLRE
                     LMNRNGINESHSSENSPYKQPFMTSFRIIAYQEAIERHENILVPHSNFRIEYSIDLMP
                     DYKNYVESYLQEHGHYPSDYQHTRDCYELFAEEIKEKMPKLPFCQDFFRLLGVMNTFS
                     YFYATLDKMGKQPDLLPLTSTMQVSVPKAFPPIPVRYFETKPIDLRFKQVLQAIQANL
                     GSESALDELLFACFNTPRQRMTLPPRLVNEIKSTIEAIVKNHLLAAGTSEAMLELNQS
                     ELKNYSQLATQLLTQQLQSAQFNIADTVKKSTLMLAQEERTSLAPLPLPARIQASIGK
                     LEAHTETLLARWNANPALAQTEIFDILTNNMMVTVNKETGEKIKFIEFVRNKFLQIEN
                     SINEELPKQIEYIIKENEKEKNHIILILKNIADTKNEAAQHIAKRQTTINEAGNAIAA
                     IHEANVKNNGLIVQVNNSIAQVSQEFDTWYASIPPRLISLNQAIISQNRASRDQEIAR
                     LNAIVQEISNKIAQNNEFISKNNQLIATLQQDIANINAELPASINAFIREFFNDENQG
                     LIEQIISVRLADVAGDLAPCIAKLNQHFKTKNDQAIEECNKRVSSKTILNELCKQEAE
                     AYLKQIMTGEISRLSRHLSLLQHMVANWMTHEKLMNFPIAKTYTYSVIGFTGEALDKK
                     TGDKFKIIGGCGVSLPNISTRVLAKSAQFCDALVHAFGESLKDMAEFTVDGRAYTALR
                     IGVIEKSTAEDLLSDEQSSLCDVFSRVMNTGESLAITNAGLSDVVDQSGASLIHYIAS
                     FLNADQTRLVIETHPEQLYKRDLFGHLPIHAAAQDNQADVVKYLLQKDPTLLEATNQR
                     HATPIITAIEYGCRNSVDTLLASGANVNHLLPNGLFPLMLAIQNNFSDIAIALIKNGR
                     ELNLNQKLDSGLTALHVAIENQMRDVVLLLIVGGSALDIPRQSDGYTAWHTAAALGEL
                     AMMQAMVARGVSIKSVLESQKNALHLASEAGHLEVVNYLLSLGVPNTKTSDGDTPLML
                     ALKAGQSQVAERLAQVIDLNTTNKLGQSASLLAIQYGQVAVSDCLIARGENPLIEDVH
                     GNSYLYYLVRNGEFHRFANLIESHSIPLDKPIHGHSLIHIAAQYGHFEIVYYLLDQGC
                     SFIAVNNSPDLLGYAVMADEIGYLREQFSGNSAPLAKLAAQAGSVRCLRFLLDKLSKE
                     EIIAAELLKIAITNGHVTAVDAIIKKQGDMRCVIDHLGNTPLHLAAATGNTQIIELVV
                     KSGADFLIQNAKQQTAFHLAFANNDAQSFKKLLRLSQPAEWPADLWSMNSIRLDSNLK
                     KLLVKYEKRLPKTALQQKISANITAESVLDDRIQGERRQELISLLNDQEFDDAIALLS
                     QPGSQFGEIKNLNEVLRLTLENSYDYAELFDSQDDELTPDTDLPWKPDRLLAFLKSKG
                     MDPSCFTGRRNPLLSILASSNDEEACYRLTLFAKVFPECIAALVQDKVISGKNCAELA
                     LIKRFNQFFDQCDSLCRLTQAHESPNYNALHQAVASDNYELTKQLLSRTSVVDCLNAK
                     GQTPLMLAALQGNVPIAQLLIARGAQPDRRDHKQLNSLHYAILGKSEAVALHILPLLR
                     QRHHASQEGMTPLLYAAQQGMLSVVSALCSDSNVLNQVDFLGRNALHLAALHGHVHVI
                     KYLAQRGFGLDQVECPSSEKRSQRSLKRTALHYAALKAQLDAVVTLLQFGADMYATDA
                     VGNGLCEYAVISQDPEMLRFVQNMSLYHQPERAGKILHAGVMVDHPEVVSEMILNGRT
                     LTTLDESGNSVLHSAARNNSGEAIELLLQDDDIPLNLKNRFGDAPLHVAARFGHVRII
                     EALLEAGADVDQKNDGGTTALILAAKEGKLGAVITLLKYQADYSIKDATDLTAAQTAL
                     LNGHLQIACRIVMAGDQSLQPVAVEQLSTENHTLLYEKMTDFERQLQRTANTASLCQL
                     GLFKSLDAHADASLLRHLTRGLHDHASSMSR"
     gene            7355..11392
                     /locus_tag="ACF2SP_001233"
     CDS             7355..11392
                     /locus_tag="ACF2SP_001233"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFD2359027.1"
                     /translation="MITPAACPDKPVLGLVDYSKFKLESSTCVATLFSQHQSAWDAFC
                     RHVDSSELNNTNRLMLMKIAFDCLHEHCNTSDLAKLLTLGKYLKDNVGTQLFFMLFSK
                     RVTPESRRLEHYIAACQLLIDLQETSGLTNLVKQSMPNLTLLVQRLEEKQATYLAEKY
                     PSRDIHETIVRFGENSFIEFPLSREELTHLKAEYIAINPWFAFLQKRSSTDLKEKALQ
                     SAEIWNERKEIEHHRRLIAIIAELIRRHYNIFPHDTQMLAFMALLNTPEHLLKGRIAQ
                     INTGEGKSTIIAMLAAYFACFDPAVYVITPTSYLAIRDQEKYQPFFNSLGFKSTNICT
                     ESPKVEQFKANIIYGTNTDFEFAMLRNSLYCLGLNDGKYTAIIDEVDNLFLDAALNSA
                     ILAIPGTEDRSWIYQPILSFANNSSSSAPVAELKNFMQSTLSPAYLEQLQQINDRQLS
                     RWLRSAREALFTKHIDRDYLVKELSTGPDIVIVDFNNTGRLNEGCHWQNGLHQFLQAK
                     HGLKITPESLSVASLSHPTYFTQFRRIIGLTGTMGQIVEREEIQRIYNVGSFDVPPHY
                     PSMRKKLPAYFAKTPDEQYDRILEHARAMRSEGRPMLVLVKTIAESVKLNTFFVNTGI
                     TPQLINLTQREDEDYLIGQAGEPGTITIATNAAGRGTDIILALNSKLAGGLRFIFGFY
                     PKNPRVEGQGDGRAGRQGQPGDCQMILCEQDDYIIFLRRWATANPGEYSERIAAWEQL
                     NADEKLSTLNSIRASFIKTESDQRYVLCQKEAIYFEKLEGFFTRNQRLYHALQNESLL
                     KSLTQTCESALQRPLSEPTVHLNTLLKGFEPLQETIDTLLMSQIHNIKVDWSVLLKQY
                     KELYMEQFQKTWAEFYDALHKENDQASLEIIRQTTDSKYQAVDQVLKIMDVNPNESIS
                     KILQILLSASLRRLLETGHCSFDFSVGSSSSPPSIVSSSSSTFSPRKITVVNMGVNES
                     LPFKLTKGFSGAEKTHRWTDGNEAVLTIPISMENNTISHIIFHTTALISNKITQKLII
                     SGKGLETKIYEYNTSRPEQQIIIDIPAELNDDMDIHFSMPNACKPCEVDPSNRDPRSL
                     AIAFKTAIISVTVQPQKKIQTSPVVQLGLFSSSDTRTLTTAIEALEAADRSEKRRLLA
                     AAYRSRAKEYESFNAFDLAQKDLVSASNICNELISEGMAFYNQTPRDFNNALKSFQAV
                     VTLNTNDKAAWWYIGQCYASLPSIASYQELSFKAFNQATTIDPEYFNAQNCKGVMLVN
                     LGRHQEAIPVLLKAASLNKDFAHTYYYLSIAFKAQGDIPKAYETMNTAAELLPASYAT
                     ARDNLKTLLDETNRLSLEEGASLQI"
     gene            12063..12869
                     /locus_tag="ACF2SP_001234"
     CDS             12063..12869
                     /locus_tag="ACF2SP_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HFD2359028.1"
                     /translation="MRKYNLRQTANQYLKLGNQGSYKIKKQRAYVIRKMIDDLYTIGD
                     VPFSWKNIQSHHIHQLVAHWKKSKIRTSTIMNHMTIVRHYLTSIDCPIPNIDNQSLQL
                     SKTTRKRKRKLKIQHDHWHSWDNPIPRLIMALQTEFGLTFQEAIYIKPEINIQADSIW
                     LTRNIAFNSLDRTIPIRFEMQKAILDKIKKLTDGKSIAEFNDYEDTKVAWRKALKKNA
                     LPINKAYRYLYAKQMAQYLLPILGKYETYWVIRSEMGIKSRDSLWRYLNE"
     gene            12862..13761
                     /locus_tag="ACF2SP_001235"
     CDS             12862..13761
                     /locus_tag="ACF2SP_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HFD2359029.1"
                     /translation="MSKSKLKNAKYSINECIKKIQNYSHASRADMKHMLNRCVKDLHE
                     LGYMITHVKGLKPKHIHILVDHWKAQNKNPATIKNYMAKLRKVASVLNKPELVKQGND
                     CYQINKRNYAPQYNKAINNVDFSKCSDPMIRLSLEAQSLFGLRREESMKIVLSDAWQG
                     NKLVIKPSWTKGGIGRDIKLTNEQQRLWLLNATKQVPAGQSLIPKEKTYKNHLAQYHE
                     VIEKMGLSKCHGLRHAYAQRRYHEITKSYDKTGNGLICPIQGGRTYRELNPLEKYWDR
                     TAREIISQLLGHSRLSITKIYLG"
     gene            complement(13794..14660)
                     /locus_tag="ACF2SP_001236"
     CDS             complement(13794..14660)
                     /locus_tag="ACF2SP_001236"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFD2359030.1"
                     /translation="MDKVKIDTNLVQKLIAEQFPQWQLLPIHSVAQSGWDNRTFHLGE
                     EMVVRLPSDKEYEPQINKEYQWLPWLSKQLSFQITHPIALGKPSPAYPLHWSINRWIE
                     GESASRQNIHDMKCFAEALGKCLREFQSLDATGGPVAGAHNFHRGGSLKAYDHEVQLA
                     IPKIKDAHEQSFASSLWQQALSSEWHLKPVWVHGDMAIGNILVRDGRLCALIDFGQLA
                     VGDPACDLVIAWNFFSGEERTAFKNAIQLDNDTWIRALGWAFWKTLCWPIKGTDVKGV
                     LHEIYSDYEMVK"
     gene            complement(14669..15700)
                     /locus_tag="ACF2SP_001237"
     CDS             complement(14669..15700)
                     /locus_tag="ACF2SP_001237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359031.1"
                     /translation="MITLEPHNPAWKAAFEVEKEQLLQLSIRNITQIEHIGSTAIPGI
                     CAKPVIDILIGVKCLSEFTSEYIQKIESLGYRYNPVFETVFPHRRYFQKDNEQGERTH
                     QIHLVTYPSSWYAKHLLFRDYLRLYPKIANEYEALKLNLSKIHDNTIDYANAKNEFCQ
                     LIDKKAFLHFGVNKPFIETARLIAFIPQVACHKDYAIMLSNPEFIQCYGVSYNEDQAL
                     NRLESDMTHYNQYGFAPWMWYDKETHNYVGRGGLKTFIFEGKEEVELTYQIAQSYWGK
                     GLAFEIGQASLEYAEEHLDLASTICFTAYNNYPSLRVMEKLGFKFEFDFEHAGITHKF
                     HRKPTIKKL"
     gene            15796..16356
                     /locus_tag="ACF2SP_001238"
     CDS             15796..16356
                     /locus_tag="ACF2SP_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359032.1"
                     /translation="MIAIKKLDELHVDELSSYLNHYQETTMFIRNNLYHSGITYQDAP
                     FHGEYYGSFENKQLNGLSLIAYHIEALGDDVNNPTLEQSIIDEIQDTQLSQNRWILFV
                     NNTPVSLCGFNANLPDIVQIGPVYTPPSLRNKGFARIAVYLCLKQAADRQVKRAILFT
                     NDNSAICAYQALGFHQIGKYRLALLK"
     gene            16380..17033
                     /locus_tag="ACF2SP_001239"
     CDS             16380..17033
                     /locus_tag="ACF2SP_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFD2359033.1"
                     /translation="MVDLEIVSLLSLISLGIVFGYIAGRYRKRAAENCGEARVRHTLA
                     KYCQNKDAHILSNITLRLEDGSTTQIDHILITTKGIFVIETKHYKGWIFAKANARSWS
                     QSLYYDKFRFQNPIHQNYRHVKAIQKTLDFIEPQCIHNIVVFSGEAVFKSAKPHNVFY
                     IEELIPAIEQYPDGVLSLNRVQFCVGRLEYLRMALTYETDVEHQAYLTQRFGNRCRS"
     gene            17444..17638
                     /locus_tag="ACF2SP_001240"
     CDS             17444..17638
                     /locus_tag="ACF2SP_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359034.1"
                     /translation="MQQQKPVQLEIEHLLFELNCCVNDLGRHRLQINTHDIQQAELSL
                     HKLESLISFVKQFDTQRIAC"
     gene            17739..18161
                     /locus_tag="ACF2SP_001241"
     CDS             17739..18161
                     /locus_tag="ACF2SP_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFD2359035.1"
                     /translation="MTASLNRIQLIGNLGADPKSIAGKEGQSFVTATLATNESFKQND
                     EWQTRVEWHQLVLFGKLTKVAEYLKKGSQVYIEGKLRSNQWTDNEGKARQALSIVVSH
                     IQLLNSPKSADESSNKTAEEHMAQMREMLQPDSEEIPF"
     gene            18227..18733
                     /locus_tag="ACF2SP_001242"
     CDS             18227..18733
                     /locus_tag="ACF2SP_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFD2359036.1"
                     /translation="MDTPSIYVACLASYNNAILHGVWIDATQSEDDIMKEIWEMLDNS
                     PEPNAEEYAIHDYEGFGSIKIHEYESISNIVEYASFIQEHGELGLALLCDYSVDDAQT
                     MLEDHYQGCYDSEADFARQLFDDCYAHQMPDSLICYFDFEAFARDLFINDYCSVESEN
                     SMHIFSTF"
     gene            18887..19027
                     /locus_tag="ACF2SP_001243"
     CDS             18887..19027
                     /locus_tag="ACF2SP_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359037.1"
                     /translation="MQSQIDKQGHSSIRTDFLMMRTSYMEINYTYILFDVAVNIVFME
                     KT"
     gene            19024..20982
                     /gene="qatA"
                     /locus_tag="ACF2SP_001244"
     CDS             19024..20982
                     /gene="qatA"
                     /locus_tag="ACF2SP_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Qat anti-phage system ATPase QatA"
                     /protein_id="HFD2359038.1"
                     /translation="MTVSPSVLFLSDHETKVDLLNNEAIARTIVQLIKEIPERPMTIG
                     VHGDWGAGKSSVLEMIEEAFTENEKMLCIKFNGWQFQGFEDAKIAVLEGIVTELIANR
                     SSLTNANEEVKNILRRIDWLKAAKKIGGLAFTAFTGLPTLEQLKSVLDTVKDKLSDPA
                     TLATKENAEAASELFSDLLKDGESRSVPKEIHEFRKAFSDLLKKAKIDRLIVLIDDLD
                     RCLPETAIETLEAIRLFVFLPKTAFIVGADEAMIEYAVRNHFPDLPESSSSQGYARSY
                     LEKLIQVPMRIPALGETETHIYVTLLAVGSRLGEDSDEFKKLLQLGRNALCNPWEGKG
                     INTSEIKTILGDKYDDVSDLLLITEQISPILSAGTKGNPRQIKRFLNALSLRLQVSNA
                     RGFGDNIRAEILAKLMLAELFLPVSVFEHIATSAANADDGICKELNILEQGERLKETS
                     KGQTKKSSNKEETSSEESSTLADWKMRSEVVKWASIEPKIGTTSLKPYLFVIKDRKNY
                     LGSTAPLSPKLMALLEKLSGGEANAMGCRQDLTSLTSNEIESLLQALRSKLLGSNAFS
                     TKPPTIFGIAELVQAHPKALQMRYVEILEELPVKQLGPWAAAGHEKIITDQIYKAQLE
                     RLIQNWRTNGSTPLKAALSAQPQNNRGQ"
     gene            20982..21848
                     /gene="qatB"
                     /locus_tag="ACF2SP_001245"
     CDS             20982..21848
                     /gene="qatB"
                     /locus_tag="ACF2SP_001245"
                     /inference="COORDINATES: protein motif:HMM:NF041924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Qat anti-phage system associated protein QatB"
                     /protein_id="HFD2359039.1"
                     /translation="MGTSTAFGGGKNSNPLIPSWLEPELIDETTTPKNDDTSTDQSDL
                     SQVASETIVSNPQPSVLPVDNRYSTARRNFTAFINSGGSDRAALGRAIVSYIHKTTGG
                     AKQAAKRMKSERQASVRLGNILSMASVNGIQSVIKNLNLENLANRPIAEIYAALIDVI
                     CPMGGDLDDSIVRDAYIEAVGEVAELGLADLEQPSTATIATIMECFISNTIKDRIMNA
                     IATKIIILPNNILIVQDIENQLKDFIRGAVSDAMTNSSFFSAGQTSRTINSIFERSFA
                     ILEALAEHEAEQ"
     gene            21845..23218
                     /gene="qatC"
                     /locus_tag="ACF2SP_001246"
     CDS             21845..23218
                     /gene="qatC"
                     /locus_tag="ACF2SP_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Qat anti-phage system QueC-like protein QatC"
                     /protein_id="HFD2359040.1"
                     /translation="MTKYILVGGFGSADAAEHKYTEKKGEQLKFLDFLTGKNSLRFGI
                     GGAIRQLAKFNIYPSEVGLDLLIFAVMVQAADTRLNRIQSSQDAWTREIRLVVPVSDT
                     ELWSKSKSVLEKILRFLTGDLWEIEFRIRPDGFENLIPQKPYLPYNYNGLSLFSGGLD
                     SLIGAIDSLESGITPLFISHAGEGAVSSPQKAIFEKLINKYTDNRVNRLRFLSARFPK
                     NLFPGIHSENSTRGRSFLFFALGAFSGSGLKKSFDLRVPENGLIALNVPLDPTRLGSL
                     STRTTHPYYIHRWNELLQTLNIPGTIFNPYWNKTKGEMVKECPNQDFLKSIVHLSVSC
                     AHPSASRYLGGGMYHHCGHCVPCIIRRAALNHAWQLGEDTTPYSPPNIFEKPLDSRKA
                     EGIQIRAFQYALANLNNKPSYLPMLIHKPGPLLEDIAILNDLKDVYKRGMKEVENFLQ
                     GVVTTPN"
     gene            23233..23973
                     /gene="qatD"
                     /locus_tag="ACF2SP_001247"
     CDS             23233..23973
                     /gene="qatD"
                     /locus_tag="ACF2SP_001247"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Qat anti-phage system TatD family nuclease QatD"
                     /protein_id="HFD2359041.1"
                     /translation="MSLELVDFHCHLDLYPNFPELVTECEHRRIYTLAVTTTPRAWQR
                     NNMLAERTKYVRAALGLHPQLIGELGHEVDVLCDLIPEARYIGEVGLDGSPQYSNSMG
                     LQKNILDKILHSCAKNGGRIISLHSRRATREVLDLLETHKKSGIPILHWFSGTLKELE
                     RAICLGCWFSVGPNMLQTKKGIELIKNIPRDKLLTETDGPFTQEKGEPNMPWNVGIAI
                     KKLSIIWKQDEALVQNQILTNLKSLCKE"
BASE COUNT         7817 a         4972 c         4709 g         6704 t
ORIGIN      
        1 aaagctcaag gatcactttt atgctccctt tcacccgagt ggttaacttt ggtagattat
       61 taccaagttc tgctattgaa gcattgacgg cttcatgaag tgcaacaact agttctagtt
      121 ttatgccttt ggctatcaag gcgttttcat cttcattgaa ctgcgagcta tcaaaaaaat
      181 tcgacatatt tcctccaaaa taaagatcat tttttgcgca cgccaccatt ataagcaaca
      241 tgaaagtatc gcacaataca gtagaattac ggtattgata catctcttgt tattaactaa
      301 aatgcacaaa aattattcat ggtaattgcg ctccctatca atgcctaagt cattatttcc
      361 gagattttta tgcctaaaaa tagcaatact accaatttgt cacaagaaga tatcgataaa
      421 gtcagagaaa gtctcgaaaa tcaatgggac agtctcgggc ttaacttatt ttcaaagtca
      481 aaaatattta aaactgcaaa aaaaataagt aaaggcatta aaacagtaga tactcacgtt
      541 aaaggggaaa agcagtatta ctggtgtggt gttgcgggtg gcaccattga tgcaggctta
      601 aatacgcttg gctccttagc aataggtgca atgacaggcg cgaatcctta tgccttgatg
      661 ctctcacaac ccgccatatc aaaaagtgca aaagcctggg gtgatcttgg gcaacgaggc
      721 tgtgatgccg ccgtcgattt ttttataaac cgatcacgct atcagtctgc taatacatat
      781 aactctcgat tcattggcct ttcaggtact aactcttttc atgcttcaag ctccttctca
      841 tcatcatcta acaacagccc ttcttttaat agctatcagg ttgagcgtac ggtaaaagat
      901 gcgattcgag gtgaaatgag tcggcttaaa cccagcatgg attctgcata tctgcgagcc
      961 catatgaatc tgggattggg ttataactta tcccgatcgg agtttcgctc cctcgcttcc
     1021 agcactaacc tcattagcag ctataatctt tttaatccaa gttcaaaact ttccgatgta
     1081 aaggcttttg ctcgtgaaat cggcggggtt gctggagaag tggcaacaat cacggatctc
     1141 atagacagcg atgcgcaggc ggaagcggat aattatattt tttgttttga atccgatcag
     1201 tttccctttt cgaaagaaat ggtttaccaa attcagcaag aattaaaaga agcatatttt
     1261 actcacaaaa cctcaccgtt tttcagttta cattttaata attttggcta tttatatccc
     1321 gtcatccacc cagctttgca aaacacgctg gttggtcaga ttattgccct gcttgattat
     1381 tggatgaaag gttttttaaa tggagggatt tatgatgaag aattcctaaa actctggcat
     1441 gaaataaggc aatgtgatga atctttttta agacaaaaga tcattgacct aaaaaaatac
     1501 tgtgaaacaa atgctgatgg cttgccttac atctctttgc gtgaactcat gaaccgcaac
     1561 gggataaacg aatcgcatag tagcgagaac tcaccttaca agcaaccgtt catgacttca
     1621 tttcgtatta ttgcgtacca ggaggctatt gaacgtcatg aaaatatcct ggtacctcac
     1681 tcgaattttc ggattgagta cagcattgat ttaatgcccg attataaaaa ctatgttgag
     1741 tcctacctgc aagaacacgg acattatcca tcagattatc aacatacacg cgattgttac
     1801 gaattattcg ccgaagaaat taaagaaaaa atgccgaagc tccccttctg tcaggacttc
     1861 tttcgtttac tcggtgtcat gaataccttt agttattttt atgccaccct cgataaaatg
     1921 ggtaaacaac ccgatttgct tcctcttaca tcaaccatgc aggttagtgt cccgaaagca
     1981 tttcccccca ttcctgtacg ttattttgaa actaaaccta tcgaccttcg ttttaaacaa
     2041 gtgttacagg ccatacaagc taacttaggc tcagaatcag cactcgatga gttattgttt
     2101 gcctgtttta atacgccacg tcagcgcatg acattaccac cacgattggt taatgaaata
     2161 aaaagcacca tcgaagcgat agtaaaaaac catttattag ctgcaggtac cagcgaagca
     2221 atgcttgaat taaatcaaag tgaattgaag aactatagcc aattggccac ccaattgcta
     2281 actcagcaac ttcaatcggc tcagttcaac atcgctgata ccgtaaaaaa atccacactg
     2341 atgttagcgc aagaggaacg tacatctctg gctcccctcc ccttacctgc ccgcattcag
     2401 gcaagcattg ggaaacttga agcgcatact gaaacactgt tagcccgctg gaatgcaaac
     2461 cctgcgcttg cacaaactga aatctttgac attctcacga ataacatgat ggtgacggta
     2521 aataaagaaa cgggagaaaa aataaagttc attgaattcg tgcgcaataa atttctacaa
     2581 attgaaaact caattaatga agagcttcca aagcaaattg aatatattat taaagaaaat
     2641 gaaaaagaaa aaaatcacat cattcttatt ctgaaaaata ttgcagacac taaaaatgaa
     2701 gcagcccaac acatcgccaa acgacaaaca acaataaatg aagctggaaa cgcaattgca
     2761 gcaatccatg aagctaacgt caaaaataac ggtctgattg tgcaggtaaa taacagcatt
     2821 gcacaagtta gccaggagtt tgatacatgg tatgcatcta ttccgcccag gcttatctcg
     2881 ctaaatcagg ccataattag ccaaaaccgg gcgagtaggg atcaggaaat tgcacgactt
     2941 aatgccattg tacaagaaat aagcaataaa atagcgcaaa acaatgagtt catttccaaa
     3001 aacaatcaac tcattgctac gcttcaacaa gatatagcga acatcaatgc agaactcccg
     3061 gccagcatta atgcatttat tcgtgagttt ttcaacgatg aaaaccaggg actgattgaa
     3121 caaatcatat cagtacgact ggcagacgtc gctggcgatc tcgccccttg cattgccaag
     3181 ctgaatcaac attttaaaac taaaaacgat caagcaattg aagagtgtaa taaaagagtt
     3241 agctcaaaaa cgattttaaa tgaactatgc aaacaagagg ctgaagccta cttaaaacaa
     3301 atcatgacag gtgaaatcag ccgactgagt cgacatcttt ccttattgca acatatggtt
     3361 gcaaactgga tgactcatga aaagttaatg aattttccca ttgctaaaac atacacctac
     3421 tcggttatcg gcttcaccgg agaggcactc gataaaaaaa ccggggataa atttaaaatt
     3481 attggcggtt gtggcgtctc gcttcctaat atatcaacca gagtattagc taaaagtgca
     3541 caattttgcg acgcacttgt ccatgcattt ggtgaatcat taaaagatat ggccgaattc
     3601 actgtcgatg gtcgagccta tactgcactg cgcattggcg tcattgaaaa atctactgca
     3661 gaagatctct taagcgatga gcaatcatca ctgtgtgatg tatttagccg agtaatgaat
     3721 actggggaat cgttagcaat caccaatgca gggctgagtg acgtagtgga tcaaagcggt
     3781 gcctcattaa ttcattacat cgcaagcttt ttaaacgcag atcaaaccag actagtcatt
     3841 gaaacgcacc cagaacaatt gtataaacgt gatctctttg gccatctacc aatacatgct
     3901 gcagcccaag acaatcaggc cgatgtggta aaatacctgc tccaaaaaga ccctaccctt
     3961 cttgaagcaa ccaatcaacg ccacgccacc cctattataa cagccattga gtatggatgc
     4021 aggaacagcg ttgatacttt attagcctct ggtgccaacg taaatcatct tttacctaat
     4081 ggcttatttc ccctgatgct cgccattcag aataattttt ctgacatagc aattgcctta
     4141 ataaaaaatg gccgagagct aaatctcaat caaaagcttg atagtggtct caccgcattg
     4201 catgttgcta tcgaaaatca aatgcgcgac gttgtactct tactgattgt cggagggagt
     4261 gcgctagaca tccctcgaca atccgacggt tacactgctt ggcatactgc agcagcgtta
     4321 ggagaattag ccatgatgca agcaatggtt gcgcgtggcg tttccattaa atcagttttg
     4381 gagtcgcaaa aaaacgcact acatcttgcg tcggaagccg gacacctgga agtggttaat
     4441 tatttactta gtctgggagt gcctaatacg aaaacaagcg atggtgacac accgcttatg
     4501 ttggctttaa aagccggtca gtcacaagtg gctgagcgcc tggcacaagt aatcgacctt
     4561 aataccacca ataaactagg ccaatctgca tcactactag ccattcaata cgggcaggtt
     4621 gcggtgtcag attgcttgat tgcacgtgga gaaaatccat taatcgaaga cgtgcacggc
     4681 aactcttatt tatattattt ggttcgtaat ggtgaatttc atcgattcgc aaatttaatc
     4741 gagagccatt cgattccctt ggataaacct atacatggtc attctttaat tcatattgcc
     4801 gcccaatacg gtcattttga gattgtttat tatttgttgg atcagggatg ctcattcatc
     4861 gctgtcaata attcacctga tttgcttggg tatgcggtga tggccgatga gattggttat
     4921 ttaagagagc agttttctgg aaatagtgcg cctcttgcca agcttgccgc acaagctggc
     4981 agtgtacgtt gtctgcgttt tttattagat aaattaagta aagaggaaat cattgctgca
     5041 gagcttctca aaattgccat cacaaatggg catgtcacgg cagtcgatgc cattataaaa
     5101 aaacagggtg atatgcgctg cgtgatagat catctgggga atacgccact gcatttagca
     5161 gctgccaccg gtaacacaca aattatagaa ctggttgtta aatccggtgc tgatttcctg
     5221 attcaaaacg caaaacaaca aacggcattt cacttggctt ttgccaataa tgatgcgcaa
     5281 tcgttcaaga aattactcag actaagtcaa ccggcagaat ggccggctga cctttggagc
     5341 atgaatagca ttagacttga ttcaaattta aagaaattat tagttaaata tgagaaacgc
     5401 ttgcctaaaa cagcgctaca gcaaaaaatt tcagccaaca tcacggcaga atctgtattg
     5461 gatgatcgca ttcaaggaga acgaaggcag gaattaattt ctttattaaa tgatcaggaa
     5521 tttgatgacg caattgcttt attatctcaa cccggatcac agtttggtga gataaaaaat
     5581 ctcaatgaag ttttgcgact aacgctagaa aactcctacg actatgcaga actatttgat
     5641 tctcaagatg atgagctcac gcccgatacg gatcttccat ggaaaccaga cagactgtta
     5701 gcctttctca aatcgaaagg catggatcca agctgtttta ccggacgtcg taatccctta
     5761 ttatcaattc tcgcatcaag caacgatgaa gaggcttgct accgattaac cctgttcgcc
     5821 aaggtttttc ccgagtgcat tgctgcatta gtacaagata aagttatttc aggaaaaaat
     5881 tgtgctgagc tggctttaat aaaacggttt aaccaatttt ttgatcaatg cgattctctt
     5941 tgccgtttga ctcaagcaca tgagagtccc aattataatg cgcttcacca ggcggtagct
     6001 tcagataatt atgagctgac taaacagctg ctttctcgaa cctctgttgt tgattgtctc
     6061 aacgcaaaag gacaaacgcc gttgatgctg gccgctttgc aaggaaatgt acctatcgcg
     6121 caattactca ttgccagagg tgcgcaacct gaccgtcgcg atcataagca attaaacagt
     6181 ttgcactatg ccattctcgg aaaatcagaa gcagttgctt tgcatatatt gccactactc
     6241 agacagcgtc atcacgcttc gcaagaggga atgacgccct tattatatgc ggctcaacaa
     6301 ggcatgttgt ctgtcgtcag tgcgctttgc tctgatagca atgtcttaaa tcaagttgat
     6361 tttctgggac gaaatgcctt gcatttggcg gccttgcacg gacatgttca cgtcattaaa
     6421 tatttggcgc aacgaggatt tgggttagat caagtcgagt gcccctcatc cgaaaaaaga
     6481 tcacaacgat cactaaaacg aacagccctg cattacgcag cattaaaagc acaactggat
     6541 gcagtagtaa cattattaca gtttggtgcg gacatgtatg cgacggatgc cgtcgggaat
     6601 gggctgtgtg aatatgcggt gattagtcaa gaccctgaga tgctccgttt cgtgcaaaac
     6661 atgtcactct atcatcaacc agaacgtgca ggtaaaattc tccatgcagg tgtcatggta
     6721 gatcaccctg aagtcgttag cgaaatgatt ttaaatggcc gcacattaac gaccctggat
     6781 gagtccggta acagtgtgtt gcatagtgcc gcaagaaaca actctggaga agctattgaa
     6841 ttattgcttc aagatgacga catcccctta aatctaaaaa atcgatttgg tgatgccccc
     6901 cttcatgttg ctgcgaggtt tggccatgta cgtatcatcg aagccctgct tgaagcaggt
     6961 gctgatgtgg atcaaaaaaa tgatggcgga acaacagcgc ttatcctggc agccaaggaa
     7021 ggaaaactcg gggcagtcat cacacttcta aagtatcaag cagactattc aatcaaagat
     7081 gcaaccgatc tgaccgcagc acaaacagcc ttactaaatg gccatcttca aattgcttgc
     7141 cgcattgtca tggcgggtga tcaaagctta caacctgttg cggttgaaca attaagtacg
     7201 gagaatcata cgctgctcta tgaaaaaatg actgactttg aacgacagct acaacgcact
     7261 gccaatacag caagtttatg tcaattggga ctctttaaat cgctagacgc tcacgccgat
     7321 gccagtttat tgcgtcatct aacgaggggg ttgcatgatc acgccagcag catgtccaga
     7381 taagcctgta ttaggcctgg tagattattc gaagttcaaa ctggagtctt cgacctgtgt
     7441 cgctacactg tttagtcagc atcaatccgc atgggatgct ttttgtcggc acgtggatag
     7501 ctcagagctc aacaacacaa accgtttgat gttgatgaaa atcgcatttg attgtttgca
     7561 tgagcactgc aacaccagcg acttggcaaa attacttacg ttagggaagt atcttaaaga
     7621 taacgttggt acacagcttt ttttcatgct tttttccaaa cgggttaccc ctgaatcaag
     7681 aaggcttgaa cattatattg ccgcctgtca attgctcatc gatttgcaag aaacgagcgg
     7741 actcacgaac ctggtcaagc aaagcatgcc gaacctgacc ttgctggtgc aacggttaga
     7801 agaaaaacaa gccacttatc tcgctgaaaa atacccaagt cgagacattc atgaaacaat
     7861 tgtccgcttt ggagagaata gcttcattga atttccattg tctcgtgaag aattgacaca
     7921 cttgaaggcg gaatacatag ccatcaatcc ctggttcgcc tttttgcaaa aacgttcaag
     7981 tactgattta aaagaaaaag cattgcaatc tgcggaaatc tggaatgaaa gaaaagaaat
     8041 tgaacatcat cgtcgattaa tcgcaattat tgctgaatta attcgtcgtc attacaacat
     8101 tttcccgcac gacacgcaaa tgctcgcttt catggctttg ctcaatacac ctgaacattt
     8161 gttaaaaggc cgtattgccc aaattaatac cggcgaagga aaatcaacca tcattgcgat
     8221 gcttgcagcc tattttgcct gttttgatcc agcagtctat gtaatcaccc caacatctta
     8281 tttagcgatt cgtgaccagg aaaaatacca gccttttttc aacagtttgg gctttaagag
     8341 tacgaatatt tgcaccgagt ctcctaaagt agaacaattt aaagcgaata ttatttatgg
     8401 aaccaacaca gactttgagt ttgcgatgct gcgaaacagt ctttattgcc tgggattaaa
     8461 cgatggaaag tacactgcga taatcgatga agtggataat ttatttttag atgccgcact
     8521 taactctgcc atattagcca tacccggaac agaagacagg agttggatat atcagcctat
     8581 tttatccttt gctaataact cgtccagcag tgcgccggta gctgagttaa agaactttat
     8641 gcaatcaacc ctgagcccgg catatctgga acaacttcaa caaataaacg ataggcaact
     8701 cagccggtgg ttaagaagcg cccgcgaagc tttatttact aaacatattg accgcgatta
     8761 tctggttaag gagttatcaa caggacctga tattgttatc gttgatttta ataacaccgg
     8821 tcgattaaac gaaggatgtc actggcaaaa tggtttgcat caatttttac aggcaaagca
     8881 cggacttaag ataacacctg aatcattaag cgtagcctct ttgtctcacc caacgtattt
     8941 cacccaattc cgccgcatca taggcctgac aggaaccatg ggacagattg ttgaacgtga
     9001 agaaattcaa cgaatttata acgtgggaag ttttgatgta ccaccccact accccagcat
     9061 gcgaaagaaa ttaccggctt attttgcaaa aacaccggac gagcaatacg atcgcatcct
     9121 ggaacatgca agagcaatga gaagtgaagg ccgaccaatg ctcgttctcg ttaaaaccat
     9181 tgctgaatca gtaaaattaa atacattttt tgtcaataca ggaatcactc cccagttaat
     9241 taatctgacc caacgcgaag atgaggacta tttaattggt caggctggcg agcctggaac
     9301 cattaccatt gccacaaatg cagcggggcg cggtacagac attattcttg cactgaacag
     9361 taagttggcg ggtggtttac gtttcatctt tggattttac ccaaaaaatc caagagttga
     9421 aggtcagggc gatgggcgag cagggcgaca aggtcagcct ggtgattgcc aaatgatttt
     9481 atgcgaacag gatgattata ttatatttct gagaaggtgg gcaaccgcaa atcctggtga
     9541 gtactcagaa cggattgccg catgggagca attgaatgct gatgagaaat tatcgacttt
     9601 aaatagtatt cgtgcatcgt ttattaaaac tgagtcagat caacgttatg tattatgtca
     9661 aaaagaagcc atttattttg aaaagctcga aggtttcttt acacgtaatc aaagacttta
     9721 tcatgcctta caaaatgagt cccttttaaa atcattaacg caaacctgtg aatcagcgct
     9781 gcaacgtcct ctttcagaac caacagttca tttgaacacg ttattaaagg ggtttgaacc
     9841 gcttcaagaa accatcgaca ctttgctgat gagtcaaata cataatatta aggtcgattg
     9901 gtctgtcttg ctgaagcagt ataaagaact gtatatggaa caatttcaaa agacctgggc
     9961 agaattttac gatgccctgc ataaagaaaa cgatcaagcc tcactggaga tcataagaca
    10021 aactacagac tcaaaatacc aggcagtcga tcaagtacta aaaatcatgg atgttaaccc
    10081 aaacgaaagt atcagtaaaa tattgcagat attattaagt gcttcactac gacgtctgct
    10141 agaaactgga cactgttctt ttgacttctc agttggttcg agttcgtctc caccgtctat
    10201 tgtttcatca agttccagta cattttcacc caggaaaata actgttgtta acatgggggt
    10261 aaatgagtct ttacctttca aattaacaaa aggattttca ggtgcagaaa aaactcatcg
    10321 ctggacagat ggtaatgaag cagttttaac gataccaata tccatggaaa ataatacaat
    10381 tagtcacatt atatttcaca caactgcgct tatttccaat aaaattactc aaaaattgat
    10441 tatctcagga aaaggtttag aaactaaaat ctatgaatat aatacgtcca gaccggaaca
    10501 acagataatt atagatattc ctgcagaact aaatgatgat atggatattc attttagcat
    10561 gccaaatgca tgcaaacctt gtgaagtaga ccccagcaat agagatccaa gatcgttagc
    10621 aattgcattc aagacagcga tcattagtgt tactgttcaa cctcaaaaaa aaatacagac
    10681 atctcctgtc gttcaacttg ggctattcag ctcttcagat accaggacat taaccacagc
    10741 aatcgaagca ctcgaagcgg ctgatagatc agaaaaacgt cgattgttag cagcagctta
    10801 tcgctccaga gcaaaagagt atgagtcttt taacgccttt gatttagctc aaaaagattt
    10861 agtgagtgca agcaatatct gcaatgaact catatcagag gggatggctt tttacaatca
    10921 aacaccccga gactttaaca atgcgctaaa atcgttccag gccgtggtga cgctgaatac
    10981 aaatgacaag gcagcatggt ggtatatcgg ccagtgttac gccagcctac ccagcattgc
    11041 cagctaccaa gaattatcat tcaaagcgtt taaccaggct accacgattg atccggaata
    11101 ttttaatgca caaaattgca agggagtaat gctggtaaat cttggacgtc atcaggaagc
    11161 tattccagtc ttgttaaaag cggctagctt aaataaagac tttgctcaca catattatta
    11221 cttgtcaatt gcattcaagg cacagggcga tatccctaaa gcttatgaga ccatgaacac
    11281 ggcagcagaa ttgcttccag catcttacgc tacggcaagg gataatctaa aaacgctact
    11341 agatgagacc aatcggctct ccttagagga aggagcgagc ttacagatat agaaaccata
    11401 ggaggaaaag gaatctcagg cgacgctctc actagtccat gccacattac gccgggcaga
    11461 taaccgttgg ctaacttgca cctcatgcat tctgcgtcac tgcaattttc cagcagtgac
    11521 gcagaaaaat caccttgatt ttaccgcgta aaatcaacac atttcagcat tttgtccgat
    11581 cccgccaatc gggattggac aaaattcttt ccaaaatccc ttgcgaagca agcaccgctt
    11641 tgctaatttg gaattaaaca tgactgatga gctggtgaaa gtccggcgaa acgtcaaccg
    11701 acgtcttatg ctaagccact tcattcccaa accccgtggc tggacgttca gtagggaaaa
    11761 ccatctagga atctctgtca tccggcaggg caactccctc gggagtttaa gtcaatttat
    11821 ttcaatttga ccatggactt gtatgacaag agccattgtc ttgtcgtttt tattaaccat
    11881 caaaacaagg agaaaataag cacccaaaga ctgttgtaaa tcggcgagat cgtttgcctt
    11941 ataacgattg atttgccccc cacttcgagc ttaatggctt tggccaggtt tcatctttga
    12001 gtgagccgct cgttcaacac taaaccaaag acagggcaaa taaattttta tcggatggga
    12061 tgatgagaaa atacaactta agacaaactg ccaatcaata tttaaagctc ggcaatcaag
    12121 gcagctacaa aatcaaaaaa caaagggcat acgtcattcg aaaaatgatc gatgatctct
    12181 atactattgg tgatgtacct ttttcctgga aaaatatcca atcgcaccat attcaccaac
    12241 tggtagccca ttggaaaaag agtaaaatac gtaccagcac gattatgaat catatgacta
    12301 tcgtcaggca ttatctaacc agcatagatt gccccattcc caatatagac aatcaatccc
    12361 tacaacttag taaaaccacc cgaaagcgta aaagaaaact caaaatccag cacgaccact
    12421 ggcactcatg ggacaatcca ataccacgcc tcatcatggc attacaaaca gaatttggcc
    12481 tgacctttca agaggccatt tatataaaac ctgaaattaa catccaggca gacagcattt
    12541 ggttaaccag aaacatcgca tttaattcct tagacagaac aatccctatc agatttgaaa
    12601 tgcaaaaagc catcctggat aaaatcaaaa agctgactga cggtaaatcc attgctgaat
    12661 ttaacgatta cgaagatacc aaagttgcct ggcgaaaggc tttaaaaaaa aatgccctac
    12721 caattaacaa agcctaccgc tacctctatg caaaacagat ggctcaatat cttttaccca
    12781 tattggggaa atatgaaacc tattgggtta ttcgcagcga aatgggcatc aaatctcgtg
    12841 attctttatg gaggtatctc aatgagtaag tccaaactta aaaatgccaa gtactcaatc
    12901 aatgaatgta ttaagaaaat ccaaaactac tcccacgcca gccgagcaga tatgaaacac
    12961 atgttgaatc gctgcgttaa agatctgcac gaattaggct acatgatcac acacgtaaaa
    13021 ggattaaaac caaaacacat acacatcctg gttgatcact ggaaagccca gaataaaaat
    13081 cctgcaacaa taaaaaatta catggctaaa ctgcgcaaag tggcctcagt gctaaacaaa
    13141 ccggagctgg ttaaacaagg aaacgactgc taccaaatca acaaacgaaa ttatgctccc
    13201 caatataata aagcaataaa taatgtcgat ttttccaaat gctcagaccc aatgatccgt
    13261 ttatcgctgg aagcccaatc cctatttggt ctccgccgtg aagaatcgat gaagattgta
    13321 ctaagcgatg cctggcaagg aaataaattg gttataaagc ccagttggac aaaaggtgga
    13381 ataggacgtg atattaaact aacgaatgag caacaacgac tatggctact caatgctaca
    13441 aagcaagttc ctgctggaca atcgctcatt cctaaagaaa agacttataa aaatcatcta
    13501 gctcagtatc atgaagtgat agaaaaaatg ggcttaagca aatgccatgg ccttcgccat
    13561 gcctatgctc agcgaagata tcatgaaatt acaaaatcct atgacaaaac tggtaatggc
    13621 cttatatgcc caatacaagg tggtagaaca tatagagaac ttaaccctct tgaaaaatat
    13681 tgggacagaa ccgcaagaga aattattagc cagttgcttg ggcattcgag attgagtatt
    13741 acaaaaattt atttgggctg acagttttca acacataatt tcattcgata tggttactta
    13801 accatctcat aatcggaata aatttcatgc aaaactcctt ttacatcagt acctttaatt
    13861 ggccagcata aggttttcca aaatgcccac cccaaagctc gtatccaagt atcattatcc
    13921 aattgaattg cgttcttaaa tgctgtcctt tcttcgccag aaaagaaatt ccatgctatt
    13981 accaaatcac acgcaggatc acctacagca agttggccga aatcgattag tgcacacaac
    14041 cttccatcac gaactaaaat attgccaata gccatatctc cgtgaaccca aacaggtttt
    14101 aagtgccatt cagaagagag tgcctgttgc cacaatgatg aagcaaagct ttgctcatga
    14161 gcatctttaa ttttaggaat tgcaagttgc acttcatgat cataagcctt taacgaacca
    14221 cctcgatgaa aattatgtgc gcctgccaca ggcccacccg tggcatctaa agattgaaat
    14281 tctcttaaac acttccctaa tgcttccgca aaacatttca tatcatgaat attctgtctt
    14341 gaagcagact ccccttcaat ccaacgattg atactccaat gcaatggata agctggtgat
    14401 ggttttccta gtgcaatagg atgcgtaatc tgaaatgaaa gctgtttgga aagccatgga
    14461 agccattgat attctttatt tatttgtggc tcatattcct tatcactagg caaacgaact
    14521 accatttcct cacctaaatg aaacgttcga ttatcccaac cactttgagc aactgaatgt
    14581 atcggtagta attgccattg cggaaattgc tcagcaatca gcttttggac taagtttgtg
    14641 tcaattttta ctttatccat tacaacctct atagtttctt tattgtaggt tttctatgaa
    14701 acttgtgcgt aatccctgca tgttcaaaat caaactcaaa tttaaaacct aatttttcca
    14761 taacgcgcaa agatggataa ttattgtatg cagtaaaaca aatagtgcta gccaaatcca
    14821 aatgctcttc ggcatactcc aaggatgctt gccctatttc aaatgctaaa ccttttcccc
    14881 aatagctttg agctatttga taagttaact caacttcttc ttttccctca aatataaaag
    14941 tctttaatcc ccctctccca acgtagttgt gggtttcttt gtcataccac atccagggag
    15001 caaaaccata ttggttgtaa tgcgtcatat ccgattccaa acgatttaaa gcctggtcct
    15061 cattgtatga aacaccataa cattgaataa actcaggatt tgaaagcatt attgcatagt
    15121 ctttgtgaca agctacttgg ggaataaatg caataagacg agctgtttca atgaatggtt
    15181 tattaacgcc aaaatgtaaa aaagctttct tatctataag ctgacaaaac tcattcttag
    15241 cattggcata atcaatcgta ttgtcatgga ttttgctcaa gttgagctta agtgcttcat
    15301 attcgttggc aattttgggg taaagacgca aataatcgcg aaacaacaga tgcttcgcat
    15361 accaagaaga gggataagta accaaatgaa tctgatgcgt tcgctcaccc tgttcattgt
    15421 ctttttggaa ataacgccga tgtggaaaaa ctgtttcgaa aaccgggtta taacgataac
    15481 ccaatgattc aattttttgg atatattcgg aagtaaattc actcaaacac ttaacaccaa
    15541 ttaaaatatc aattactggt tttgcacaaa ttccaggtat agctgtactg ccgatatgct
    15601 caatttgggt tatattcctg atactcaatt gtaatagctg ttctttttca acttcaaatg
    15661 cagctttcca tgcaggatta tgaggctcta aggtaatcat attctaagat tttgtattta
    15721 ataataatca atagtgggct atcttaacat aggtcttaaa agtgtattaa aaatattcat
    15781 cataccgagg aaaaaatgat tgcgataaaa aaactagacg aattgcatgt agatgaattg
    15841 tcatcctatc tcaatcatta tcaagaaacg accatgttca ttaggaataa tttataccac
    15901 tcgggaatta cctaccaaga tgcaccattt cacggagaat attatggctc ttttgagaac
    15961 aaacagctta atggcttatc gcttatcgct tatcatatag aagcacttgg ggacgatgtt
    16021 aacaatccca cacttgaaca atccattatt gatgaaattc aagatacaca acttagccaa
    16081 aatcgatgga ttttatttgt aaacaacacg cctgtttctc tatgtggatt taatgccaat
    16141 ctacctgata ttgtacagat cggccctgtt tataccccgc cttcattaag aaataaaggt
    16201 tttgccagaa ttgctgtata tctttgtcta aaacaagctg ccgaccggca ggtcaaaaga
    16261 gcaattttgt tcaccaatga taattccgct atatgcgcat accaagctct tgggtttcac
    16321 caaattggaa aataccgact agcactgtta aaataatgtg cttgttgggg gtaagatgta
    16381 tggttgatct tgaaatcgtt agtttattat cattaatttc tctaggtata gtatttggtt
    16441 atattgccgg acgatatcgt aaacgggcag ctgaaaattg cggtgaagcc cgagtcaggc
    16501 acactttagc aaagtattgc caaaacaaag atgctcatat tctaagtaat attactttac
    16561 gtttagagga tggttccaca actcaaatcg atcatattct aattacaaca aaaggcatat
    16621 tcgttataga aacaaagcat tacaagggat ggatattcgc taaagcaaac gcaagaagct
    16681 ggtctcaatc gctctattac gataaattca gatttcaaaa tcccatccat cagaattata
    16741 gacacgttaa agccatacaa aagacccttg atttcataga gccacagtgt atccataata
    16801 tcgtggtttt tagcggggaa gccgtgttta aaagtgccaa acctcataat gttttttata
    16861 tagaagaatt aattcctgca atcgagcaat accccgatgg tgtcttaagt ttaaatcgtg
    16921 ttcagttttg cgttgggcga ttagagtatt tacgcatggc attaacatac gaaactgatg
    16981 ttgagcatca ggcatattta acgcaacggt ttggtaatcg ttgtaggagt taatgaagac
    17041 aacatcctgc ttgatgaatc accaactatc gaaacacatt ctctatcact aaaagagcac
    17101 aattagttca tttgggttca tttaatatca ccaagaaaaa aatcttgatt tccaatactg
    17161 gcttgatatt ctgaaagcaa cacaactgat gagtcagtgc aattctgacg aaaccaccag
    17221 gtcttgtgat atgccatctt gttcccctaa ttccatggca aagcgtttag cagggaaacc
    17281 ccaccaagag atgttttacg gctttacgcc cgaggagtgc tttaagcaca tcatcattaa
    17341 taaccaggta aatcaaccga tgtttcggtg atcttttttg cataacccaa ttgggatgaa
    17401 cagtcctttt tggggctaat cctccctacc ctaaggagct accatgcaac aacagaaacc
    17461 agtacaactt gaaattgaac atttgctctt tgagctaaat tgttgtgtga atgatttagg
    17521 ccgtcatcga cttcaaatca atacccatga catacagcaa gctgagttaa gtttacacaa
    17581 attggaatcc ctaatttcct ttgttaaaca gttcgatact caacgaatcg cttgttaatt
    17641 gaggtaataa aagtcttatc cgcataacag aaaaccacct gcctatgcgg atgtttttct
    17701 gttatgcggc tcacattaaa aaataacagg agaatacaat gactgcatct ttaaatcgca
    17761 ttcaattaat cggtaatctc ggtgctgatc ctaaaagcat cgccggaaaa gaaggacaat
    17821 cttttgtcac cgccactttg gcaactaatg aatcattcaa gcagaatgat gaatggcaaa
    17881 ccagagtgga atggcatcag ctggtgcttt ttggcaaact gactaaagtc gctgaatatt
    17941 taaagaaagg ttcgcaagtt tacatcgaag gtaaattgcg ttccaatcaa tggactgaca
    18001 atgaaggcaa agcgcgtcaa gcattaagta ttgttgttag ccacattcag cttctcaact
    18061 caccaaaatc agctgacgaa tccagtaaca aaaccgcaga agagcacatg gctcagatgc
    18121 gtgaaatgtt gcaaccggat tcggaagaaa ttccgtttta atcataccct agcacgggag
    18181 caagctcccg tgtgggattg ctccattcac ataaggagat aatcccatgg acaccccttc
    18241 aatctacgtc gcctgtttag catcgtacaa taacgccatc ctgcatggtg tttggataga
    18301 tgctactcaa agcgaagacg acatcatgaa agaaatctgg gaaatgttgg ataacagccc
    18361 agaacccaat gctgaggaat acgccataca cgattatgaa ggattcggca gcatcaaaat
    18421 tcatgaatat gagtcaataa gtaacattgt ggaatacgct tcatttattc aagaacacgg
    18481 agagttagga ttagccctac tctgtgatta ctcggtagat gatgcacaaa ccatgttgga
    18541 ggatcattac caaggttgtt atgattcgga ggctgatttt gccaggcagt tatttgatga
    18601 ctgttatgcc catcaaatgc cggatagttt gatctgctat tttgattttg aggcttttgc
    18661 tcgggatctt tttatcaatg attactgttc ggttgaatct gaaaattcaa tgcatatttt
    18721 ttcaaccttt tagccccata atatggggca cctatctaag accactaaaa taaaaatgga
    18781 ttttcagtta aataattgca cccatcagcg ttgaaaaaac aaattttgac tatatattgt
    18841 aaagaccata gcagattaac aatctttctc atcaaggagg tgagatgtgc aatcacaaat
    18901 agataagcaa ggccactcat ctattcgtac cgatttttta atgatgagaa ccagttatat
    18961 ggaaataaat tacacttata tactgtttga cgtagctgtg aatattgtgt ttatggagaa
    19021 aacatgactg tgtccccatc tgtacttttt ttatctgatc atgaaactaa ggttgacctt
    19081 ttaaataatg aggcgattgc tcgtacaatt gtgcaattga ttaaagaaat tccagagcgt
    19141 ccgatgacta tcggtgttca tggtgactgg ggagcaggaa aatctagcgt acttgaaatg
    19201 attgaggagg ccttcactga aaacgaaaaa atgctttgta taaaattcaa tggctggcaa
    19261 tttcagggat ttgaagatgc taagatcgcc gttcttgaag gaatagtaac agagctaatt
    19321 gctaatcgat catcactgac aaacgcaaat gaagaagtaa aaaacatatt acggcgtatt
    19381 gattggttga aagcggcaaa aaaaataggt gggttagctt ttactgcttt tactggactc
    19441 ccaactttag agcaactaaa aagtgtcctc gatactgtta aagacaaact atctgatcct
    19501 gcgacactgg caacaaaaga gaatgcagaa gcggcatccg aattgttttc tgatttgctg
    19561 aaagatggcg agagtcgcag tgttcctaaa gagatacatg aatttcgtaa agcatttagt
    19621 gatttattaa aaaaagcaaa gattgatagg cttattgttt tgattgatga ccttgatcgc
    19681 tgcttacctg agacagcaat cgaaacatta gaagctattc gtttgtttgt ttttttgcca
    19741 aaaacggcat ttattgtagg agctgatgag gcaatgattg aatatgcggt gcgcaatcat
    19801 ttcccagatc ttccagaatc aagtagctcc caggggtatg cacgtagtta tcttgagaag
    19861 cttatacaag tacctatgcg aattcctgcg ttaggtgaaa cagaaacaca catatatgta
    19921 acattgcttg cggtaggaag ccgtttagga gaggattcag atgaatttaa aaagcttctt
    19981 caattaggaa gaaatgcttt gtgtaaccca tgggaaggta aagggataaa tacttccgaa
    20041 attaaaacta tactgggtga taagtatgat gatgtatcag atttactttt aatcacagag
    20101 caaattagcc caattttatc agctggcaca aaaggaaacc ccagacagat taaacgattt
    20161 ctaaacgcac tcagtttaag acttcaagtt tctaatgctc gcggctttgg tgacaacatc
    20221 agagctgaga tcctggcaaa acttatgctt gctgaattgt ttttacccgt ttcagtattt
    20281 gaacatattg ccacttcagc agcaaatgca gatgatggaa tatgcaagga acttaatatc
    20341 ttagaacaag gggaacggct aaaagaaacc agcaaaggac aaacaaaaaa atctagtaat
    20401 aaagaagaaa cctcatctga ggaaagctct acacttgctg attggaagat gcgctcagag
    20461 gtagttaaat gggcgtcgat agaacctaaa attggcacaa cttcgctgaa accatattta
    20521 ttcgttatta aagatcgtaa aaattatctt ggatctacag ccccattatc tccaaaacta
    20581 atggccttgc ttgagaaatt atctggagga gaggcaaatg ctatgggatg tagacaagat
    20641 ttaacgagtt taacatcgaa cgaaattgaa tctttattac aagcccttcg ttcaaaacta
    20701 ctaggaagta atgcgttttc tactaagcca cctacaattt ttggtattgc tgagctggtg
    20761 caagcccatc cgaaagcact tcaaatgcga tacgtagaga tacttgaaga actacccgtc
    20821 aaacagcttg ggccatgggc agctgctggc catgaaaaaa taataaccga ccaaatatat
    20881 aaagcacaat tagaacgact cattcagaac tggagaacta atggttcgac tccattaaaa
    20941 gccgctttgt cagcacaacc tcaaaacaat agaggtcaat aatgggaact tctacagcct
    21001 ttgggggtgg caaaaactct aatcctctca ttccaagctg gctggagcca gagttaatag
    21061 atgagactac gacccccaaa aatgatgata cttcgactga ccaatctgat ttatctcagg
    21121 ttgcttcaga gacaatagtt agtaatcccc aaccaagtgt gttgccagtc gataatcgtt
    21181 atagcactgc gcgtcgtaac tttactgcat ttataaattc agggggatct gatcgcgctg
    21241 cgcttggacg agctattgtc tcctatatac ataaaacaac aggtggtgca aaacaagctg
    21301 ctaaaagaat gaaatcagag cgtcaagcca gtgtacgact tggcaacatt ctttcaatgg
    21361 caagtgtcaa cggtattcaa tccgttatta aaaacctcaa tttagaaaat cttgctaatc
    21421 ggcctatcgc tgaaatttat gcagcgctaa tagatgtaat ttgcccaatg ggtggtgatc
    21481 tggatgattc tattgttcga gatgcgtata tagaggcagt tggggaagta gccgaattgg
    21541 gattagcaga cctggaacaa ccatcaacag ctacaattgc taccatcatg gaatgtttca
    21601 tctcaaacac cattaaagat cgtatcatga atgcaattgc cacaaagatt attatattac
    21661 ctaacaatat tttgattgtg caagatattg aaaatcaatt aaaggatttt attcgtggtg
    21721 ctgtctctga tgcgatgaca aatagtagtt ttttttctgc tggccaaaca agtagaacta
    21781 taaattctat atttgagcgc tcgtttgcaa tccttgaagc acttgcggaa catgaggcag
    21841 aacaatgaca aaatatatat tggtgggagg attcggtagt gctgacgcag cagaacataa
    21901 gtatactgag aaaaaaggcg aacaactaaa atttttagat tttctgacag gaaaaaactc
    21961 tctccgattt ggtattggag gcgcgataag gcaattagct aaatttaata tttatccgtc
    22021 tgaagtggga cttgatctcc tgatatttgc tgtaatggtt caagctgccg atacccgatt
    22081 aaatcgtatt caatcttcac aggatgcatg gacgcgtgaa attcgtctag tcgtacctgt
    22141 gtcagatact gagctttggt caaaaagcaa atcagttcta gaaaagatat tacgtttctt
    22201 gactggcgat ttatgggaaa ttgaatttag gattagacct gatggattcg agaatctaat
    22261 cccccaaaag ccatacttac cttacaatta taacggatta tcactttttt caggcggcct
    22321 tgatagcctt attggagcga tagattctct tgaatcgggt attactcctc tttttataag
    22381 ccatgcaggt gaaggagctg taagtagccc gcaaaaagct atttttgaaa aattgatcaa
    22441 taaatatact gacaataggg tcaatagact acgctttctt tcagctcgtt tcccaaaaaa
    22501 tttatttcca ggaatacatt cggaaaactc tacgcgagga agatcatttc ttttttttgc
    22561 tttaggtgct ttttcgggct ccgggttaaa aaaatcattt gatttacgtg tcccagaaaa
    22621 tggtttgata gcacttaatg taccactcga cccaacacgg ctaggttctt taagcacaag
    22681 aacaacacat ccgtattata ttcatcgatg gaatgagtta ttacaaacat taaatatccc
    22741 aggtacaatt ttcaatcctt actggaataa aacaaagggg gaaatggtta aggaatgccc
    22801 aaatcaggat tttctgaaat ccatagtcca tttatcagtt tcttgtgcac acccctctgc
    22861 tagccgatat ctcggtggag ggatgtacca tcattgtgga cattgtgtgc cctgtattat
    22921 aagacgggca gcattaaatc atgcatggca attgggtgaa gatacaacgc catatagccc
    22981 ccctaatatt tttgaaaaac ccttggattc aagaaaagct gagggcatac aaattagagc
    23041 attccaatac gctcttgcaa atttgaataa taaaccttct tatttaccta tgcttataca
    23101 taaaccggga ccattattag aagatatagc aattcttaat gacctaaagg acgtctataa
    23161 acgtggtatg aaggaagtgg aaaacttctt acaaggcgta gtaactacac caaattagaa
    23221 atgaggtatg ttttgtcatt agaattagtt gattttcatt gccatttgga tctttatcca
    23281 aactttcctg agctagtcac tgaatgtgaa cataggagaa tatatactct tgctgttaca
    23341 actactccta gagcatggca aagaaataat atgttagccg agcgaacgaa atatgtacgt
    23401 gcagctcttg ggctacaccc tcaattgatt ggcgaactcg gccatgaagt tgatgtctta
    23461 tgtgacttaa tacctgaagc tcggtatatt ggagaagtcg gcttggatgg ttctcctcaa
    23521 tattctaaca gtatgggctt acaaaaaaat attttagaca aaatactaca ttcatgtgca
    23581 aaaaatggtg gccgcattat aagtttgcat tcacgacgtg cgactagaga agttttggat
    23641 ttactcgaaa ctcataaaaa atcaggaata cctatccttc attggttctc aggaacttta
    23701 aaagaattag aaagggctat ttgccttgga tgctggttca gtgtaggacc aaatatgtta
    23761 cagactaaaa aaggaataga attaatcaaa aacattcctc gagataaatt gttaactgaa
    23821 acagatggac catttactca ggaaaaaggg gaaccaaaca tgccttggaa tgtaggtatc
    23881 gccattaaga aattgagtat aatatggaag caagacgaag cactggttca aaatcaaata
    23941 ttaactaatc taaaatcttt atgtaaagaa taatgactta caaacaatat aatctcttac
    24001 tttaataaat aatacaaaaa taccccttcg ggagatcgtc gtatttgagt acgacatcgt
    24061 acgacacagt acgccatttg taggaaaaac ccgcggcaaa atttataata aaatcaagtg
    24121 gttaaaattg ttaagcatga ctcataatcc gttggtccta ggttcaagtc ctagtgggcc
    24181 caccaattaa agccagtaaa at
//
LOCUS       DAXLDT010000041        23640 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXLDT010000041 DAXLDT010000000
VERSION     DAXLDT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23640
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.041"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(33..323)
                     /locus_tag="ACF2SP_001248"
     CDS             complement(33..323)
                     /locus_tag="ACF2SP_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359042.1"
                     /translation="MQHTIFVERERTADFQTTKGLLDILGRNGNKDDIMAFLIERNLP
                     VDEIGADQLAEQIINLPPKLGYLTISNALQWRLQYSRVIQQAGNVDGITRIR"
     gene            complement(421..687)
                     /locus_tag="ACF2SP_001249"
     CDS             complement(421..687)
                     /locus_tag="ACF2SP_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359043.1"
                     /translation="MINKLTIKELKQEAAIFAEIETKYDEPGLYGVTDGKAVGTYLEH
                     KFTAYLLEKYVCEKGNSASGIDLPGLEVDIKVTSIKQPQSSCPL"
     gene            complement(756..2054)
                     /locus_tag="ACF2SP_001250"
     CDS             complement(756..2054)
                     /locus_tag="ACF2SP_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359044.1"
                     /translation="MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLMGDASKGL
                     FLVFLTFFAGYLARPIGAIMMGLLSDIYGRKIILAASILTMGLSTSLIGFIPPNNTIG
                     IISVLLLLSLRVIQSFSCGAEYLNSSTYLVENAETTKKGFTGSWASFGAMAGMMLASL
                     VTLSLTYFVDNYPEHEWFIWRIPFVLALLGSTIGLYIRLCIPESMEYIMYYADRPKPK
                     LRNLFSETVQYIKTRKLQSLYVFFLSSLGVTTTFQIYIYGPMQARLSGNFSDQQIMTS
                     NIISLIVLLCLFPLVGRLSDKVNRERIMIAASFGFLVLSIPFFYSLSKGDFHQLLLGQ
                     TMISIPAGAYYATVPVILAEMFPLKLRCSVLSVLYSTAASLSAGLAPLISFILVKKTL
                     ISTSPSILTIILVVIIYIILCMKFYMVNKYNKIFLTNSVQ"
     gene            complement(2060..2578)
                     /gene="def"
                     /locus_tag="ACF2SP_001251"
     CDS             complement(2060..2578)
                     /gene="def"
                     /locus_tag="ACF2SP_001251"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFD2359045.1"
                     /translation="MNTLLDKDDPILRQTAEPIAESEFGSSWLKELIKAMFGIMADKG
                     AVGVAAPQIGISKRVIVFSTEYTKRRKPEYPIPDTALINPSLKILSKEIQIGHEGCLN
                     CGEIMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
                     SLTTLSAMQSKG"
     gene            complement(2559..3173)
                     /locus_tag="ACF2SP_001252"
     CDS             complement(2559..3173)
                     /locus_tag="ACF2SP_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgJ"
                     /protein_id="HFD2359046.1"
                     /translation="MQFKKFVDKDVTAVEVTLHPKAKEFLFEHFVTMRKVFSDVLGQV
                     ETDYISIALINQTGQIFFLSSKPSIEQNLIEKHLWLFDGCYQSSFFSQDKPKLWNELL
                     HKGHTELIKKYKQIEPGLITGISIPAEYDSYKVIFSFGLKRINPYIQSKTSILCEKLL
                     AMGKYALRQIQDYLTFPDKLPYTIAKPKLKLIINNQVTYEHTPG"
     gene            complement(3163..3654)
                     /locus_tag="ACF2SP_001253"
     CDS             complement(3163..3654)
                     /locus_tag="ACF2SP_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2359047.1"
                     /translation="MSKGLYQSFANRLISTLKDKGYTASRSPNGICIKTLAEFTGASE
                     QICRRYIRGDALPDYEKVKQLALHLTVSPGWLLFGEDDHAVSKENEVDEKLLHYILKH
                     SHHLYPVSHGSNDDYADFVLGLIKEVKAIDTSENNLLKIIDLALGSISSYEEKRKKHS
                     HAV"
     gene            3864..4109
                     /locus_tag="ACF2SP_001254"
     CDS             3864..4109
                     /locus_tag="ACF2SP_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359048.1"
                     /translation="MFHDQKITIYKGIIQYLLDSTNYSLQRIANLSNSSVAHLQLIHQ
                     YGRLPKESKVEINLLKLFTTVTDMELKGEWKARVLLK"
     gene            4133..4339
                     /locus_tag="ACF2SP_001255"
     CDS             4133..4339
                     /locus_tag="ACF2SP_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2359049.1"
                     /translation="MQPVNELFTQQVLFINDVVAIIGKDRLTLRRWWTDGRFPKPVKL
                     HGTTLVWHKDVIEEWINHNIRRTF"
     gene            4581..5708
                     /locus_tag="ACF2SP_001256"
     CDS             4581..5708
                     /locus_tag="ACF2SP_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2359050.1"
                     /translation="MGRKRIPGIYKKGSHWQIDKKIFGYRLCESTGTGDLEEAEKYLA
                     RRIDEIRQATVYGVRPKRSFMEAATKFLMENQHKRSIDDDAGRLREVVKYIGDLSLES
                     IHIGSLQPFIEGRRKDGVSTRTINHGLKVVRRILNLAASEWMDEFGLTWLANAPKIKL
                     LPEHDLRKPYPLNWDEQHRLFDELPQHLEKMALFAVNTGCRDQEICELRWEWEIKIPE
                     MPHIMVFIIPAELVKNGEERLVVCNDTARSAVNGERGKHPTHVFSFKGRPLARILSTG
                     WRNARVKSELPHVRVHDLKHTFGRRLRAAGVSFEDRQDLLGHRSGRITTHYSSAELQN
                     LFDAANRVCEKQKSGVVLTLLRNTNFKTRKVANSGVSAISL"
     gene            complement(5799..5875)
                     /locus_tag="ACF2SP_001257"
     tRNA            complement(5799..5875)
                     /locus_tag="ACF2SP_001257"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5839..5841),aa:Ile,seq:cat)
     gene            complement(5965..7830)
                     /gene="rpoD"
                     /locus_tag="ACF2SP_001258"
     CDS             complement(5965..7830)
                     /gene="rpoD"
                     /locus_tag="ACF2SP_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFD2359051.1"
                     /translation="MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHF
                     DVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVR
                     MYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
                     LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPE
                     QAKIYFDELREAFEKAMKALKEHGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHF
                     RQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLSWLETIISKHAKKLDLDR
                     IEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLNRISRQILQETGREATPEELAEKMELSEDKIRKVLKIAKE
                     PISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAKVLRM
                     RFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG"
     gene            complement(7917..9650)
                     /gene="dnaG"
                     /locus_tag="ACF2SP_001259"
     CDS             complement(7917..9650)
                     /gene="dnaG"
                     /locus_tag="ACF2SP_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFD2359052.1"
                     /translation="MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHN
                     EKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGT
                     GEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
                     HNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKD
                     QKPKYLNSPETVLFQKSRELYGLHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVA
                     TLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLP
                     NEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYL
                     QKMVEGSYKQLLIDDLARLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLL
                     QNPEIYSIAIQQINPALLHEQEHHILLKLLQQLKDKPNANTATLIEFWRNSSYFELIV
                     KLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERLSLQNLL
                     KERHGQAEIEK"
     gene            complement(9659..10102)
                     /locus_tag="ACF2SP_001260"
     CDS             complement(9659..10102)
                     /locus_tag="ACF2SP_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2359 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359053.1"
                     /translation="MTIKEQINNDIKDAMRAKDKNLLNALRLISAAVKQIEVDERIEV
                     DDERMLVILDKMSKQRKESIAQFEKASRDDLVAQEQFELNVISKYLPEPLTDNEIEEL
                     ISDAIKSTGAEKMSDMGKVMAILKPKLQGRADMAQVSARIKAKLS"
     gene            complement(10305..10544)
                     /gene="rpsU"
                     /locus_tag="ACF2SP_001261"
     CDS             complement(10305..10544)
                     /gene="rpsU"
                     /locus_tag="ACF2SP_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFD2359054.1"
                     /translation="MPTVRVKEGENPEYALRRFKRSCEKAGILTELRRREFYEKPTAE
                     RKRKQAAAVKRHLKKISRDVSSRRGVGHRRKKSTT"
     gene            10749..11750
                     /gene="tsaD"
                     /locus_tag="ACF2SP_001262"
     CDS             10749..11750
                     /gene="tsaD"
                     /locus_tag="ACF2SP_001262"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFD2359055.1"
                     /translation="MLVLGIESSCDETGVAVYDSESGLLSHALHSQIATHRVHGGVVP
                     ELASRDHVNYLVPLVDEVLTKAQIRKNQLDGIAYTAGPGLIGALLVGSCFAKSLAYAL
                     NIPALAIHHLEAHLLAAKMETPSLDFPFIALLVSGGHCQLIEVNNIGEYRLLGDTLDD
                     AVGEAFDKTAKLMGIPYPGGAVLANLADQCLSTPYQFPRPMTDRPGLDFSFSGLKTHA
                     LNTWNQSEKKESDRSEIAKAFQQAVVETLIIKCKRAIKESQSKRLVVAGGVGANKALR
                     SALQKWIKDIKGEVYFPALEYCTDNGAMVAYAGCLRMMRGESDGGWGVMVKPRWPLA"
     gene            complement(12049..12882)
                     /gene="plsY"
                     /locus_tag="ACF2SP_001263"
     CDS             complement(12049..12882)
                     /gene="plsY"
                     /locus_tag="ACF2SP_001263"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFD2359056.1"
                     /translation="MALFIFLILVGYLMGSINSAIIVCRTFGLPDPREEGSKNPGATN
                     VLRLGGKQYGIMVMVFDALKGILPVILAKLLSAEPVTVAFTALAAVVGHMYPVFFHFR
                     GGKGVATTIGALLAFHFVIGVMVAATWLLVANFWRYSSLASIASISLAPFYSLILVGN
                     LNIFPPLFMITILVLYKHRDNFNRLIDGKEPKIKFKHSVIEEIMEASPATSAEQEFPG
                     KEVIDTNIDETEKTEQAEAVKKPKAKKATTKAKKTASKEETTKKPRSTKPKTKTVKEK
                     E"
     gene            13236..14645
                     /locus_tag="ACF2SP_001264"
     CDS             13236..14645
                     /locus_tag="ACF2SP_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFD2359057.1"
                     /translation="MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINAL
                     TERIPPEECLKQAKEIDKSIASKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGE
                     KAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
                     SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNL
                     ISFGPMARSVSDLRLGLSVLAGSDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVD
                     AEIQNVVKSAALALQDDVAMVREVRPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGV
                     NNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTAAK
                     PHGIGIKEISDFSYAMTHNLSGFPTISLRCGTSAKGLPINILIAANRWKDTTSLAVAE
                     RLEQLMGGYKPPSLIHSLQ"
     gene            complement(14677..15804)
                     /gene="hemW"
                     /locus_tag="ACF2SP_001265"
     CDS             complement(14677..15804)
                     /gene="hemW"
                     /locus_tag="ACF2SP_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFD2359058.1"
                     /translation="MLPLSLYIHIPWCIRKCPYCDFNSHKSPDVLPEHQYVQALIEDL
                     KTDISSYNTREINSIFIGGGTPSLFSAEAYNNLFNELKHMLPFAKNIEITMEANPGTV
                     EQHRFTDYRQSGINRLSLGIQSFNPNHLKILGRIHDEKQAHSAIDTARKAGFDNLNLD
                     IMHSLPNQSVTQGLQDLKTALSYQPEHLSWYQLTIEPNTVFYKHTPPLPSEEEDYLLE
                     EQGFALLHNSGYNRYEISAFSKPEKQARHNINYWLFGDYLGIGAGAHGKMTTPNAIIR
                     TRKHRQPKDYLDNEKPYLAARETITGKELLFEFMLNTTRLEQSIPLELFTTATGLELN
                     QLLPLLKLANKKQLINLTDTHWQVTPLGRQYTNDLQALFLP"
     gene            15886..16446
                     /gene="nudE"
                     /locus_tag="ACF2SP_001266"
     CDS             15886..16446
                     /gene="nudE"
                     /locus_tag="ACF2SP_001266"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFD2359059.1"
                     /translation="MRNKPKCKQRTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGH
                     GAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVIDRNETPIEAANRELREETGFAA
                     RELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
                     DFTEARSIAALFLVKEWLNKRKSYDK"
     gene            16436..17428
                     /locus_tag="ACF2SP_001267"
     CDS             16436..17428
                     /locus_tag="ACF2SP_001267"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFD2359060.1"
                     /translation="MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLEL
                     EPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDL
                     LQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
                     ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKE
                     TFVSTVQQRGAAVIKARGSSSAASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGV
                     DEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSERDTVKSLGLLD"
     gene            complement(17526..18446)
                     /gene="mavF"
                     /locus_tag="ACF2SP_001268"
     CDS             complement(17526..18446)
                     /gene="mavF"
                     /locus_tag="ACF2SP_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavF"
                     /protein_id="HFD2359061.1"
                     /translation="MQNIWLLCFYKLLLKKGLWRNIGMEDPFSVLLKSLQSVFHLEAM
                     RNGKYTFPAVQHLKEATENYNPKARNTFIELLRAIREALPYIEKWRINFNVIRKSMDA
                     LAKLHHMPTIDWNQVLSHPKVSPRFQFSALNHSHHEHDLIPWLENKVGKPFAQLDHTE
                     LTQTLVDNRDRLGFSQKLSNQLKKDPDFLYNIILRSERNFIKISQTRLILYLTDEQLA
                     RAIIKHIPVLMHKRKAPFEQIEQLIHTLNEILSNGRSVSTLLRNTDAKTILENSPLFQ
                     MYLSEEYKNRHQHPNKDPDLQTKESLKPGL"
     gene            18577..19116
                     /locus_tag="ACF2SP_001269"
     CDS             18577..19116
                     /locus_tag="ACF2SP_001269"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFD2359062.1"
                     /translation="MITVGNKFPEFQLKATVANDINNAFQMISNETYRGKWLVVFFWP
                     KDFTFVCPTEIAEFGRLNSEFADRDAQILGGSTDSEFVHLAWRNQHPDLKDLPFPMLA
                     DVKRELTANLGILDDEEGVAQRATFIVDPQGITRFVMVTDLNVGRNPAEVLRVLDALQ
                     TDELCPCNWKKGEETIHIK"
     gene            19139..19654
                     /locus_tag="ACF2SP_001270"
     CDS             19139..19654
                     /locus_tag="ACF2SP_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFD2359063.1"
                     /translation="MLENVKESIPEVAKDIRLNLTKVLDLEQSDGLSEHQILGSALAV
                     SYSLGNEVLIRGLKDLVHNEQIVRAAQLAASLMAMTNIYYRFVHLTEIKEIEHIPAGL
                     RMQGMMTPGVDRVTFEILSLAVSALNGCGACVSSHARQLKEHGLSDTSLARVGRITAV
                     IHAVHVSGRIK"
     gene            complement(19698..20186)
                     /gene="sodC"
                     /locus_tag="ACF2SP_001271"
     CDS             complement(19698..20186)
                     /gene="sodC"
                     /locus_tag="ACF2SP_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodC"
                     /protein_id="HFD2359064.1"
                     /translation="MNKSGIILISTILFSSMAIADDLTAPIYTTGPKPVAIGKVTFTQ
                     TPYGVLITPDLTNLPEGPHGFHLHKNADCGNHGMDAEGHYDPQNTNSHQGPYGNGHLG
                     DLPVLYVTSNGKAMIPTLAPRLKLSDMHNLAVMIHANGDTYSDNPPQGGGGDRIACGV
                     IK"
     gene            20491..22515
                     /locus_tag="ACF2SP_001272"
     CDS             20491..22515
                     /locus_tag="ACF2SP_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2359065.1"
                     /translation="MELRIDNTPFKAIFQPLDLGFTQIKNRLLMGSMHTGLEEDKENL
                     NRLASFYRERAQGGVGLIVTGGFAPNRAGRLAPFAAKLTNSKEQHRHEIITQAVHEAG
                     GKIALQILHAGRYGYHPFIVAPSGIKSPISPFKPWEMSKRRIIKTIKHFARCARLAQL
                     AGYDGVEIMGSEGYLINQFIVTHTNHRRDEWGGSFSNRIRFPIEIVRSVREAVGEHFI
                     IIYRLSMLDLVAEGSNWQEVVTLAKAIEQAGASLINTGIGWHEARIPTIGTMVPSAAF
                     TQVTQHLKPEIKIPVITSNRINTPELANQLLEDGVADMISMARPFLADPCFAEKAKLG
                     ESKAINVCIACNQACLDQVFVNKTASCLVNPRACNETTLVYETTNQPKAIAVVGSGPA
                     GLAFAVTAAERGHKITLFEKANQIGGQFNLAKKIPGKEEFQHTLNYFAYQLQKYKVDV
                     RLNTEATVELLKDYDEVVLASGIIPRVPEIKGIDHKKVVSYIDVITQNKPVGSRVAVI
                     GAGGIGFDVAEFLTHDHQLTSEQFYNEWGIDISGQHRGGLKPPEIAPSPREVYLLQRK
                     KEKMGKRLGKTTGWIHRSSLKHKQVKMFSGVNYERIDDQGLHLTIDDKPQVLEVDSIV
                     ICAGQIELKNLYEPLKMAGKLVHLIGGAYKALELDARHAIDQACRLAALV"
     gene            22512..23429
                     /locus_tag="ACF2SP_001273"
     CDS             22512..23429
                     /locus_tag="ACF2SP_001273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFD2359066.1"
                     /translation="MSKIAVVINPIAGGGRGKKIWKVLKPGLHALFEQTVYRISNRVN
                     DLEAITDGLLAENPDSLLIIGGDGTLNHVLNGLIENDKLKNPQIKIAFFNAGCGGDFT
                     RQFPQQKMTEFLDRLSHNQFIKCDIGKITLANQVSRYFINIASCGLSGHVVLRVSKSK
                     WLKKLGGTLNYLLHALTGLMTYRKTKVRVQIDDALPFDSSILLMAVCSGQYFGGGMHV
                     APIAKVNDGLFDVVSFNEFSKLNALLKLYKIYSGGHLLDNNVHYVQAKKIKIEPLEES
                     KIEIEADGEIIGCLPAQFELIKETLPLIV"
BASE COUNT         6856 a         4367 c         5040 g         7377 t
ORIGIN      
        1 ccaaattgag atttatctaa attttttttc atttacctaa tccttgtaat gccatcgaca
       61 tttcctgctt gttgaattac acgtgaatat tgaagtctcc attgtaatgc gttggaaata
      121 gtaagatatc ctaatttggg tggcaaattt ataatttgct cagctaattg gtccgctcca
      181 atttcgtcaa caggtaaatt tctttcgatc aaaaaagcca taatgtcatc tttatttcca
      241 tttctaccaa gaatatccaa taagcccttt gttgtttgaa aatcagcagt tctttctcgt
      301 tcaacaaaaa tagtatgttg catatccaag cggcctgttt tattagtatg gtcatcatat
      361 ttttcatata caaatattaa aagattataa ccaagaccgt atactttttg tgttgctgat
      421 ttaaaggggg catgaagatt ggggttgttt gatgctagtg acttttatat caacctctaa
      481 accaggtaag tcaattccag atgcagaatt tcccttttca cagacatact tttctaaaag
      541 atatgcggtg aatttatgct caagataagt tccaacagct ttaccatcag ttacgccata
      601 aaggcctggt tcatcatatt ttgtctcaat ttcagcgaat attgctgcct cttgttttaa
      661 ttcttttatt gttaacttat taatcatttt tttctcgatt gttaagtttc tttccgacgt
      721 cactcataaa gtaaacaatg aattatttaa ctggttcatt ggacagagtt tgtcaaaaat
      781 atcttattgt atttattaac catgtaaaat ttcatgcaca atattatgta aataataacc
      841 actagaataa ttgtcaaaat cgaaggagac gttgaaatta aagtcttttt aaccaatata
      901 aaagaaatta gtggagcaag tccagccgat aaacttgcag ctgttgaata cagtaccgat
      961 aatacactac atcgcagctt aagtggaaac atctcagcta aaatcaccgg tacagttgca
     1021 taataagctc ctgcggggat agaaatcatg gtttggccta aaagtaactg gtgaaaatca
     1081 ccttttgata gtgaataaaa aaaaggtatg gatagaacaa gaaaaccaaa acttgcggca
     1141 atcataatcc tttctctatt tactttgtca gatagtcgcc ccactaatgg gaataaacaa
     1201 agcagtacga ttagtgaaat aatattagag gtcataattt gttgatcaga aaagttaccc
     1261 gacaagcgtg cttgcatcgg tccatatata tagatttgaa atgtggtagt gactccaaga
     1321 ctacttaaga aaaaaacgta tagtgattgg agctttcttg ttttgatgta ttgtactgtt
     1381 tctgaaaata agtttctcaa ttttggtttt ggcctatctg cataatacat aatatattcc
     1441 atactttcag gtatacataa cctgatatat aaaccaattg tggagcctag caaagcgagt
     1501 acaaatggta tgcgccaaat gaaccattca tgttcaggat aattgtctac gaaataagtc
     1561 aaagaaagtg ttaccaacga tgccagcatc attcctgcca tcgctccaaa tgaagcccaa
     1621 ctaccggtaa accctttttt tgtagtctca gcattttcta caagatatgt tgatgagttg
     1681 agatattctg ctccacaaga aaaactttgt atgactctga gcgagagcaa caataggact
     1741 gatatgattc cgattgtgtt attagggggt ataaaaccaa ttaatgatgt ggatagtccc
     1801 attgttagga tggatgccgc taggataatc ttccttccat agatgtcaga taataaaccc
     1861 atcattatag cgccaattgg acgagcaaga tatcctgcaa agaaagttag aaaaacaaga
     1921 aataaccctt tagatgcatc acccattagg tatttggcaa tatagactga gagtaggcca
     1981 caaatggcca tgtcaaacga ttcaattaaa ttacctaatg tacctgccag tatgattcgg
     2041 gtttgatgct tcatgcgatt tatcctttac tttgcattgc cgataaggtg gttagtgaat
     2101 cttgatcttc cactcggtct aaaaataaaa atccatccag gtggtcaatt tcatgctgaa
     2161 ggatacgcgc ttctaagccg gaagctttct tagtaattct atttccatct atatcaaagc
     2221 cggaatattc aatctccata gctcttggaa cctcgcccat tatttcgccg caattaaggc
     2281 agccttcatg accaatttga atttccttgg aaaggatctt taaggaagga ttaattaaag
     2341 ctgtatcagg aatgggatat tcaggttttc ttcgtttggt atattcagta ctaaatacaa
     2401 taactcgttt gctgatccca atttggggag ctgcgacacc cacagcgccc ttgtcagcca
     2461 taatgccgaa catggcctta atcaactctt ttaaccagct gctaccaaat tctgattcag
     2521 cgattggctc agcggtttgt cttaaaatgg gatcgtcctt atccaggagt gtgttcatag
     2581 gtcacctgat tgttaatgat taatttcagt tttggtttgg ctatcgtgta tggcagtttg
     2641 tcaggaaagg ttaaatagtc ttgaatttgc cttaacgcat atttccccat ggccagtaat
     2701 ttctcgcaaa ggattgatgt tttactctga atgtatggat ttatccgttt taatccgaaa
     2761 gagaaaataa ctttataaga atcgtattca gccggaatag aaatgccggt aattagtcct
     2821 ggttctattt gcttatattt ttttattaac tctgtatgcc ccttatgaag aagttcgttc
     2881 catagtttag gtttatcctg gctaaaaaag ctggattgat agcagccatc aaacagccag
     2941 aggtgcttct caattaaatt ctgttcgatg gatggtttag aggacaggaa aaatatttgt
     3001 cctgtttggt taataagagc aatagaaatg tagtctgttt ctacttgccc tagtacatca
     3061 gagaacacct tgcgcatggt gacaaagtgt tcaaagagaa attccttggc tttggggtga
     3121 agcgttactt ccacagctgt tacatcctta tctacaaact ttttaaactg catgactatg
     3181 cttttttctc ttttcttcat aggaagaaat tgaaccaaga gccaagtcaa tgatttttag
     3241 taaattattc tccgaagtgt caattgcttt gacttctttg atcaatccta acacaaaatc
     3301 ggcgtaatcg tcattactcc catgagatac gggatataag tggtgactgt gttttaagat
     3361 gtaatgaagc aatttttcat cgacttcatt ttctttagat accgcatgat cgtcttcgcc
     3421 aaaaagcagc cagccagggc ttaccgtaag atgtaaagcc agctgtttga ctttttcata
     3481 gtcgggtaaa gcatcccctc gtatgtaacg gcgacagatc tgttcggacg caccagtaaa
     3541 ctcagctaga gttttaatac atatcccatt tggagaacgt gaggcagtat atcctttatc
     3601 tttgagagtg ctaatcaagc ggttagcaaa cgattgatag agtcccttac tcataaaatt
     3661 atccttgtaa tcgaacgtca taaaaaaatt atcatataaa acggtatcat tcaatatcat
     3721 atcgtatatt gatttaaatt tctttaaaac acatattgat aataaaatta atatcgtttt
     3781 aatataagtt tcgtcaaatg atgctaaatg ttaatttgac gcgaattgga ctttagtaca
     3841 catgttttac aggagtatgt cgcatgtttc atgatcagaa gatcaccatc tataaaggga
     3901 tcattcagta tttactggat tccaccaact attctctaca acgaattgcc aacttatcca
     3961 actcgtcagt agctcatttg caattgatac accaatatgg tcggttacca aaagaaagta
     4021 aggttgaaat caatttattg aaattgttta ccacggtaac tgatatggaa ctcaagggtg
     4081 aatggaaagc acgtgtgctt cttaagtaac aaaaggattt ggagaatcaa taatgcaacc
     4141 tgtgaatgaa ttatttacac aacaagtttt gtttattaat gatgttgtag ccatcattgg
     4201 caaagatcgt ttgacattac gtcgctggtg gacggatggg cgttttccta aacccgtgaa
     4261 gttacacggt acaactttgg tatggcataa ggacgtgatt gaggaatgga ttaatcacaa
     4321 catcagacga acattttgat tcaggatgaa atgatgagca gatttaacat attaccaaga
     4381 ttaatacgtc tcagagacgc accagcatac gtgggcatgg atcgacatcg ttttaatgaa
     4441 gaagtacgac ctggcatacg agaaattgtt attggtacac agggtgtggc ttttgatcga
     4501 cttgatttag acgcgtgggt agacgattat atacagtgta gtgatcgacc cgcggcatcc
     4561 aagcgtagga gtttggaatt atgggacgaa aacgaatacc agggatctac aaaaaaggta
     4621 gtcactggca aattgacaaa aaaatctttg gatacagact ttgcgaaagc actggtacag
     4681 gcgacctcga agaagccgag aaatatctag caagacgaat tgatgaaatt cgccaagcaa
     4741 cggtatatgg tgtcaggcca aagcgaagtt ttatggaagc cgcaaccaaa tttctaatgg
     4801 aaaatcagca taaacgcagc attgatgacg atgcagggcg tttacgtgaa gttgtaaagt
     4861 acattggtga tttgtcgtta gaatctatcc acataggttc gttgcaaccc ttcattgaag
     4921 gcagaagaaa ggatggtgta tcaacgagaa ccattaatca tggtttgaag gtggtacggc
     4981 gaattctgaa tctggcagca agcgaatgga tggatgagtt tggcttaacc tggttggcta
     5041 atgcgcctaa aattaagctc ttaccggagc atgatttgcg caagccttat ccactgaatt
     5101 gggatgagca acatcgactc tttgatgagt taccgcaaca cttggaaaag atggctttat
     5161 ttgccgttaa tactggttgt cgggatcaag agatatgtga gctgcgttgg gaatgggaaa
     5221 ttaaaattcc tgaaatgccg catattatgg tgtttatcat accagctgag ttggtcaaaa
     5281 atggtgaaga acgactggtc gtttgtaatg atacagccag atcagctgtg aatggagagc
     5341 gtggaaagca tccaacgcat gtgtttagct ttaaaggtag acctttagcc cggattttat
     5401 caacgggttg gcgaaatgcc agagttaaat ctgaattgcc acatgtgcga gtgcatgatt
     5461 taaagcatac ctttggccgt cggttacgtg ctgctggggt aagttttgaa gacagacagg
     5521 acttgttagg acatcgttct gggcgaatca ccacgcacta ttcatcggct gagttacaga
     5581 acctatttga tgcagccaat agagtttgtg agaagcaaaa gagtggggtg gttttgactt
     5641 tgttgcggaa cacaaatttt aaaaccagga aggtagcaaa tagtggtgta agtgcaattt
     5701 cgctttagat tttgcacgca ggtcccgaag aagtcccgaa gcgggtttct aggggtttaa
     5761 ggtatcaagt gtaacttatt gattttactg actttaattg gtgggcccac taggacttga
     5821 acctaggacc aacggattat gagtccgctg ctctgaccaa ctgagctatg ggcccggaga
     5881 gggggtcatt ttaaacttaa tttctttaat gttctactat tttttatatt tttttaatga
     5941 ctgtttatat aaacagtcat taaattaacc ttcatcccct tcaaggaagc ttcttagctt
     6001 ttctgaacgt gaaggatggc gcaatttgcg tagtgctttt gcttcgattt ggcgaatacg
     6061 ttcgcgggtt acgtcaaatt gtttaccaac ttcttccagg gtatggtctg tattcatttc
     6121 gataccaaaa cgcatacgca gaaccttggc ttctcgtggg gtcagggttt ccagtatttc
     6181 taaagtggct tctcttaaac cttccgctgt agccatatcg attggcgatt caatgttgtt
     6241 gtcttcaata aaatcaccca gatgagagtc gtcatcatca ccgacagggg tttccatgga
     6301 aataggctct ttggctatct ttagaacttt gcgaatttta tcttcactta gctccatttt
     6361 ttcggccaat tcttccggtg tcgcttccct gccagtttct tgcaaaattt ggcgagaaat
     6421 acgattgagc ttattgatgg tttcaatcat atggactgga atacgaatag tccgcgcctg
     6481 atcggctatg gatcgtgtaa tcgcttgtct aatccaccag gtcgcatagg tagaaaactt
     6541 atagccacgg cggtattcga atttatcgac tgctttcatc aggccaatgt tgccttcctg
     6601 gatcaaatca agaaactgca agccgcggtt ggtgtatttt ttggcgatgg aaatcacgag
     6661 acgcaagtta gcctcgacca tttctttttt agcacgcctt gctttggctt cgccaataga
     6721 catcttgcgg ttaatgtctt tgatttcact aatggttaaa ccatattcga tttcaaaaga
     6781 ggccagcttg gattgtaagc gtagtatttc ttcccgattt tcttctatac gatcaagatc
     6841 aagttttttg gcatgtttgc tgataatggt ttctaaccag cttagatctg tttcctggcc
     6901 tgggaaggta tctataaaca attttctggg gatgcgtgct ttttcgatac aaagacgcat
     6961 tatgcttctc tcaaattcac ggatatgatt tcgcaattgg cgaaaatgac gagttagttt
     7021 atcaacttgt ctggaagtca atttaagttt caaaaaagat tccgacatgg aatcgagcaa
     7081 ctcaaccgtt tttttattcg tacggccatg ctctttgagg gctttcattg ccttttcaaa
     7141 ggcctcacgc agctcatcaa agtaaatttt tgcttgctca ggattgggtc catcatccac
     7201 atcagatata ccaccttcgc cgtcttcatc ttcctcatcg acatcaagaa gtatttcttc
     7261 ctgttgctct tcatccagca tggaaccaat gttagtagca ggtgctattt cttcatcaac
     7321 atccgcaaaa ccactaatga tttcattgag gcgaatttct tccgcaagga aacgatcata
     7381 gtcttcaaga accagttgca ctgtttccgg ataatgcgcc agggatttta gaacctggta
     7441 aattccttct tcaatgcgtt tggcaatgcg gatttcacct tcacgagtca gcagttctac
     7501 tgtgcccatt tcgcgcatat acatacgaac cgggtcagtt gttctgcctg tttctttatc
     7561 cacagaggcg agtaccatgg ctgcttcttc aatatctggg acttcctcag tatttaataa
     7621 gagcgcaagt tcatcttcac tgggtggaag ctcaaaaact ttgatgttca tgccttccag
     7681 catgctaatg attacatcaa aatgctctgt atccacaata ttaggtaata agtcattaat
     7741 ttgactgtaa gtcaaataac cttgctcttt acccaggcta atgactttcg taatttgtga
     7801 gctttgctga tcttggtcat tcatggtcat aggcacttct gttggaacaa tgggaaatta
     7861 caaaaagttt taaattataa actgggtggc tgcaacatac cagtatttca gtatttttat
     7921 ttttcaattt cagcttgccc atgtctttcc tttaataaat tttgtaaact gagccgttca
     7981 gcttctgtta agccagtttt tctggatttt tctatgtatt gtcgtatcaa tatctcacga
     8041 ttttgttttt gtaaaaataa taagacatca ataaattctt tagtcagctc ctgctcaggc
     8101 acttgatgat cccaggccgc caatttaact attaattcaa aataactgct attgcgccag
     8161 aattcaatga gggtggcagt attggcattg ggcttgtctt ttaattgttg caataatttc
     8221 aataaaatat gatgttcctg ttcatggagc agagcaggat tgatttgttg tattgctatt
     8281 gaataaattt cggggttttg taagaggagc gcgatagcaa tacgcagagg tgaacgagaa
     8341 atagcagctt gtacttccgg cttgctttcc gatttatcag taatcaaatt agttaaccga
     8401 tggctttcaa tatgagttaa gcgtgccagg tcatcaataa gcaactgttt gtatgagccc
     8461 tcaaccattt tttgcaaata gggtttcact gcattgataa gctgtgtttt tcctgcaggg
     8521 tttgacaaat taatgtcttt acttaaagta tcaaagaaaa agcggtggag tggtgttgct
     8581 tgttgcaaga gctcaaggaa accgtttttt ccttcatttc ttaccaggct atcagggtca
     8641 tgttcattcg gcaagaaaat aaatccggca tctaatccgg cattcaaatg cggtaaactg
     8701 ctctccaatg ctcgccaggc tgcttgttta cctgccgcat caccatcaaa gcaaaaaatg
     8761 agatgtttgg tatgctttgc caatagttgt atatggaatg tgctggtggc agttcccaac
     8821 gttgccacca cattaaaaat accatgttgt gccaaggcaa tgacatccat gtaaccttca
     8881 acaacaataa tactgtcggg atttttctgc tgagacagga cttgatgtaa gccatacaac
     8941 tcacgacttt tttgaaaaag tactgtttca ggcgagttaa ggtatttggg tttttgatct
     9001 ttatcgagta cacgtccgcc aaaaccaatc acacgaccgt ttctgtcatg gatggggaac
     9061 ataattcgat tacgatatct gtcataaatt ttaccctcat cgcttttaat gagcataccg
     9121 gtactcaata gctcacgctg attgtttggg aaagcttttt ccaggttgtg ccaaccttca
     9181 ggtgcatagc ctaaatgata tcgtttggca atttccccac taagacctcg gttgcgaagg
     9241 tagtcaattg ccacttgtcc attatatttt aatttttttt ggtaatattg gctgacttct
     9301 gacatgagat tatagagatt gagagatgat ttatgtttct ctcctgtacc atcccttggg
     9361 acacttaaac ccaagcgcgt cgcgagggtt tcaacggcat ccgtaaaacc ttgattcaga
     9421 taattcatta caaaactgat ggcgttacct gaagcaccac aaccaaaaca atgataaaat
     9481 tgtttttttg caacaacatt gaatgatggt gttttctcat tatggaaagg gcaacacgca
     9541 atatgactgt tgcctctttt tttcaaagga acatatccgt ctattaattc caccaaatca
     9601 gtgcggttta atagatcatc aatgaatggc tgtgggatta agccagacat attggaactc
     9661 agcttaattt cgcctttatc cgggcgctga cttgggccat gtccgcgcgt ccttgtaatt
     9721 ttggtttaag gatagccatg actttaccca tatcagacat tttttcagcg ccagtagact
     9781 tgatggcatc gctaattaat tcttcaattt cattgtcagt taaaggttca ggcaggtatt
     9841 ttgaaatgac attcaactca aactgttcct gggcaacgag atcgtcacgg cttgcttttt
     9901 caaattgagc aatagattct ttgcgttgtt tggacatttt atccaggatg actaacatgc
     9961 gctcatcatc cacttcaatg cgctcatcga cttcaatttg ttttaccgca gcactgatta
    10021 agcgtaaagc attgagcaag tttttgtcct ttgcgcgcat agcgtcttta atgtcattgt
    10081 tgatttgttc ttttatagtc attttaattc cttaaaaaac aaaaggtcac atacaatgtg
    10141 accttggtgt aatataggac gagctgcgat ttactaccga aactgccacg aaggccaatt
    10201 tcaaataaaa acaattaaaa ataacagagc ttccaaaaca gggcactgtt taagtgcctc
    10261 acgcaaactt gctttttagt gcagtcaatc ttgaaaaaat cagatcaggt agtgcttttt
    10321 ttacgtcggt gaccaacacc tcgtcttgaa ctaacatcac gagatatttt tttaagatga
    10381 cgttttactg cagcagcttg cttgcgctta cgctctgcgg ttggtttttc ataaaactca
    10441 cggcgacgta attcggttaa aataccggct ttttcacaag agcgtttgaa acgtcgtagt
    10501 gcatattctg ggttttcgcc ttcttttaca cgaacggtag gcattaacta gtcctctggt
    10561 tatttaatct taataggtgg gcaattctag tgctagattt tccacacgtc aagattaatc
    10621 tatacgtttc ttgctatttt agcaaattat caagacattg ttctttaatt taatggtatc
    10681 ttttgtctgt gatataattg aaaaatgatt aaaatctgga aaattgattc cgattattga
    10741 ggtagttcat gcttgtattg ggtatagagt cttcctgtga tgaaacaggt gttgctgtct
    10801 atgattcaga atcaggatta ttgtcccacg ccttgcattc acaaattgcc actcatcgtg
    10861 tacatggcgg tgttgttcca gaactggcat caagagatca tgtgaattac ttggttcctt
    10921 tagtcgatga ggtactaact aaagcgcaaa ttcgtaaaaa ccaattggat ggaatagcct
    10981 acaccgcagg acccggatta attggcgctt tgttagttgg ttcctgcttt gccaaaagtc
    11041 tcgcctacgc cttaaatatt ccggcattgg caattcacca tttggaagcc catctgttag
    11101 ccgcaaaaat ggaaacccca tcccttgatt ttccctttat tgctctgtta gtttccggtg
    11161 gccattgtca actaatagaa gtaaacaaca ttggtgagta tcgattatta ggggatactc
    11221 tcgacgatgc agtaggagaa gcctttgaca agacagccaa actgatgggg attccctatc
    11281 ctggaggagc cgtgttggca aatttggctg atcaatgttt atcaactccc tatcaatttc
    11341 cccgccccat gactgacagg ccaggcttgg actttagttt tagcggacta aaaactcatg
    11401 ccttaaatac ctggaaccaa agtgaaaaaa aagaaagcga ccgctccgaa attgcaaaag
    11461 cattccaaca agcagtagtt gaaacactaa ttataaaatg caaaagagcc attaaagagt
    11521 cgcaatcaaa gcgccttgtg gtagccggtg gagtaggggc caataaagcc ttgcgtagcg
    11581 ctttgcaaaa atggattaaa gacatcaaag gggaagtgta tttccccgct ctggaatatt
    11641 gcacagataa tggtgcaatg gtcgcttatg caggatgttt gcgcatgatg cggggggaga
    11701 gtgatggggg gtggggggtt atggttaaac ctcgttggcc cctcgcttga acgtccgtct
    11761 tcggcgcatc ttgccagatc agtgctctaa aaaatgctca tgtacttatc tgtacactcc
    11821 gcttttttag tcgcaatgat ttgtcaacct gcactcgaat acgggcgttc aagggtaggt
    11881 gtcttcggcg catcttgcca gatcagtgct ctaaaaaatg ctcatgtact tatctgtaca
    11941 ctccgctttt ttagtcgcaa cggcttgtca acctgcgctc gaatacgggc gttgaagggc
    12001 aagttgtctt tggcgcatct ttgcttgtgc attaataagt gctgcgcctt attccttttc
    12061 tttgactgtt ttcgttttag gtttggttga tctcggtttt ttagttgttt cttccttgct
    12121 ggctgtcttt tttgcttttg ttgttgcttt tttggctttt ggttttttta cagcctcagc
    12181 ttgttctgtc ttttctgttt catcaatatt cgtatcaatg acttctttgc caggaaactc
    12241 ttgctctgca gaagttgcag gagaagcttc cattatttcc tctatcacac tgtgtttgaa
    12301 cttgatttta ggctcttttc catctatcaa acgattaaaa ttatctctgt gtttgtagag
    12361 aaccagaatg gtgatcatga ataatggggg gaaaatatta agattaccca ccaatattaa
    12421 ggagtaaaaa ggggctaaag agatagaagc tattgatgct aaagaggaat agcgccagaa
    12481 atttgctacc agtaaccagg tcgcggcaac catgacacca attacaaaat gaaatgctaa
    12541 caaggcacct attgttgtag caacaccttt tccccctctg aaatgaaaga aaacagggta
    12601 catatgacca acaactgcag ctaaagcagt aaaggcaacg gttacaggtt cggcgcttaa
    12661 caatttggcc aggatgacag gaagtatacc ttttaaagca tcgaatacca tgaccataat
    12721 accgtactgc ttccctccaa gacggagtac attagtagct cctggatttt ttgatccttc
    12781 ctctcttggg tcggggagcc cgaacgtacg gcacactatg atagctgaat taatggagcc
    12841 cattaaataa ccaactagta ttagaaatat aaaaagcgcc accgatttct ccttactcgg
    12901 gaataatttt tattatcaac caatctcaat taacaggcaa ttttaagata gaaaatatgc
    12961 caagactaga taatagaatt catttcatgt tatttcaatt taattccatg attagtgctt
    13021 gaatgagtgt aggaattgac ttgtttatgc aaacagcagg catagttggt ataatttgat
    13081 ccaacaagag cgttcatatt gctaaaaaag aggatgttcc atagaatttg aattatttgc
    13141 aatagtttga gacggatact gaatgtcgcc ttttagtcct gttcggtgtg tcactatcat
    13201 tctattccat tatttaaggg aattttacta aagctatgga tgccattcat ctcctttctg
    13261 caacagaaat cattcgccgc attaaaatga aagaactctc ggctgcagag gtcatggtgg
    13321 cgcacttaaa ccatattgat aagattaatc ctgtaatcaa tgcgctcacc gaacgtattc
    13381 cccctgagga atgcttaaag caggcaaagg aaattgacaa gagtatcgct tcgaaaaaaa
    13441 atctgaacaa gttaatgggg ttgcctgttg ccataaaaga cgctttgtat gtgaaaggac
    13501 taatttgttc ttcggcatgt tcaggatttt acaagggaga aaaagcagta cgtgatgcga
    13561 ccttggtttc tcgactgaaa aaggaaggag caattattct tggcttaact aatgttcctg
    13621 aattgtgtcg cggtggtgat tcagataatt taatctatgg acggaccaat aatccttatg
    13681 atctggcaag aaccagtgga ggcagtagtg gtggttccgc ggctttgatc gctgcaggag
    13741 gcgttccctt tgctttgggt tccgatggtg gaggtagttt aatgcagcct gcccattgtt
    13801 gcggtattgt tgcattgaag ccaacacatg gccatcttcc tcatacagga tctgtaggtg
    13861 gggattccta tggtcttatt ggcaatctga tttcttttgg accaatggct cgttctgtca
    13921 gcgaccttcg cttaggactc tccgtgcttg cgggctctga ccaatatgat ccttatacaa
    13981 acccggttcc tgttatgcct gctgcaccac taaaaaaatt acgtgtagcc tattttacag
    14041 agaatggttt tacgccagtt gatgcggaga tacagaacgt tgttaaatca gcagccttgg
    14101 ctttgcagga tgatgtggct atggtgaggg aggtacgacc tgattgtgtc agcaaggctt
    14161 ttgatttgca ctgggagctt tttctcgggg gcgatcgcgg agcgggattt aaaactatgt
    14221 tatcggagtt aggggttaac aatctgtcat gggaattaca agagttcttg cgtcaagcgg
    14281 aacaaactca attctcggta acccaattgc atcaacgtat gcgtgaaatc gatttgtttc
    14341 gtctggaatt agctttattt atgcaagatt atgatgtatt gattagccca gtgtttccaa
    14401 cagcagcaaa accacatggg attgggatta aagaaatcag cgatttttcc tatgcgatga
    14461 cacataattt aagtggattt ccaacgatca gcctaagatg cgggacttca gcaaaggggt
    14521 tgcccattaa tatattaata gccgctaatc gctggaaaga tactacctct ctggctgttg
    14581 cagaacgcct tgagcaatta atggggggat ataaaccccc ttctctaatc cactctctgc
    14641 aatgaagcaa gaatcaataa tttttgaaac agcgaactag ggcagaaaca aggcttgcaa
    14701 atcattagta tattgcctgc ctaatggtgt tacttgccaa tgagtatctg ttaaattaat
    14761 caattgtttc ttattagcca atttcagcaa gggtaaaagc tgattaagtt ccaatcctgt
    14821 cgctgtcgta aataactcca aaggtatgga ttgttccagc cgggttgtat tcagcataaa
    14881 ctcaaacaac aattctttac ctgtaatggt ttcgcgagca gccaggtaag gtttctcatt
    14941 atccagataa tctttgggtt gacggtgctt acgggttctt attattgcgt ttggggttgt
    15001 catttttcca tgagcaccag cgcctatacc caaataatca ccgaatagcc aataattgat
    15061 attgtgtctg gcttgctttt ccggtttgga aaaggcagaa atttcatagc ggttatatcc
    15121 ggaattatgt aacagggcaa acccttgctc ctccagcaag taatcttctt cttcggaagg
    15181 caggggagga gtatgcttgt aaaatacagt attcggttct atggttaatt gataccagga
    15241 taaatgttca ggttgatagg ataaagcggt cttcagatcc tgtaatccct gcgttacact
    15301 ttgattaggt aaactgtgca tgatatcaag attgagatta tcaaagccgg ctttacgggc
    15361 agtatctatt gcagaatggg cttgtttctc gtcatgaata cggccaagaa tttttaagtg
    15421 attgggatta aagctttgaa ttcccaaaga aagacgattg attcctgatt gtctataatc
    15481 agtaaatcga tgttgttcta cagtaccggg attggcttcc attgtgattt caatattttt
    15541 tgcaaagggg agcatgtgct ttaattcatt aaataagttg ttataagctt cggctgaaaa
    15601 taaactgggt gtgccgccac caataaaaat ggagttaatt tcacgggtat tgtatgagga
    15661 aatatccgtt ttaaggtctt caatcaaagc ctgaacatac tggtgttcgg gtaaaacatc
    15721 agggcttttg tgtgagttaa aatcacagta tgggcatttt ctgatgcacc agggaatatg
    15781 aatgtacaag gataacggaa gcattacaat ttttaaactg gcttgttagg gaatgcaata
    15841 gtatcatggt agcaattttg ctacaaaatt aagtgatgac cattgatgcg aaataaacca
    15901 aaatgtaagc aaaggaccgt gatagccaaa tcccggttgt ttacagttga agaaatgtat
    15961 ctggaatttt ccaatggggc tcagcgagtc tatgagcgga ttaaaagtca tggtcatggt
    16021 gcggtgttaa tagttgcatt gactcccgga gaatcattat tattagttcg ggaatacgcc
    16081 gcaggtactg attcttatga attatcattt cccaagggag tgatagacag aaatgaaacc
    16141 cctatagaag cagctaacag agagttacgt gaagagactg ggtttgcggc aagggaactg
    16201 cactggattc gttccatgtc attggctcct ggttattttg gagctcgcat tgagcttatt
    16261 gttgcagagg gtttatacca ttcgccgttg ccaggtgatg agcctgagcc tcctgaaaca
    16321 gtagagtggc ctttacacgc tgcttatgag ttattggaaa aaccggattt taccgaggcg
    16381 cgcagtattg ctgcgctatt tttagttaaa gagtggttga ataagaggaa atcatatgac
    16441 aaataatcga gtaagagttg ctgtcacagg tgcagcaggg caaataggtt atgccctggt
    16501 gtttcgtatt gcttccggtc aaatgtttgg gccaaatact gaagtggaat tgaatttact
    16561 tgaacttgag ccggcacttc cctcactgga aggagtcgct atggagcttg atgattgcgc
    16621 atttccctta ttaaaacgca ttgtttgtac tgcagatttg aataaggcaa tggatggtgt
    16681 taactgggca ttattagtcg gttcagttcc gcgaaagcaa ggcatggagc gttctgattt
    16741 attacaaatc aatggtggta tattcaccaa acaaggtcag gcgattaatg attatgccag
    16801 cgatgatgta cgggtatttg ttgtaggaaa tccttgtaat accaattgcc taattgctat
    16861 gaatcacgca aaagacgtgc cttctgatcg tttttatgca atgaccacat tggatgagtt
    16921 aagagcccgt actcaattgg ctaaaaaagc aggcgttgac attactgcag tgactcaaat
    16981 gacgatttgg ggtaatcatt ccgctaccca atatcccgat ttttataacg caaaaatcaa
    17041 tgggacttct gctgcgcaag tcattaatga tgaaacctgg ttaaaagaga cttttgtatc
    17101 aaccgttcag caacgaggag ctgcagtgat caaagcgcga ggctcgtctt ctgctgcttc
    17161 tgccgccaat gcgataatta caggggttaa tcatttggtt actgatactc ctgctggcga
    17221 atcgttttca atgtgccgtc gctcaaaggg agagtatgga gttgatgaag gtttgatctt
    17281 ctctttccca tgccgaagag aacatggcga attaaaagtg gttgagaacc tggagttcaa
    17341 cgattttgga cgcgagagat tcaataccac tttaaacgaa ttacgaagtg agcgtgacac
    17401 ggtcaagtct ttaggcttat tggattaacc aaaccgggtc aattcttgat tgatttgatt
    17461 ctgttgtttt tgctgattac cctgctttgt gaatttttca gggcagggtg ctgttgcacc
    17521 ttgttttata aaccaggctt caatgactct tttgtttgta aatcagggtc tttattggga
    17581 tgttgatgtc tgtttttata ttcctcactc aaatacattt gaaataatgg cgaattctcc
    17641 aggatggttt tggcgtctgt atttcgtaaa agagttgata cggaacgacc attggataat
    17701 atttcgttga gagtatgaat caattgctct atttgctcga agggtgcttt tcttttatgc
    17761 atcaaaacgg gaatatgctt aattatagct ctggcaagtt gttcgtctgt caagtaaaga
    17821 attaaacggg tttgagaaat tttgatgaaa tttctttctg atctgagaat gatattgtat
    17881 aagaaatcag ggtctttttt taattgattg ctcaattttt ggctaaatcc aagcctgtct
    17941 ctgttgtcaa ccagtgtctg ggttaattca gtatgatcca attgagcaaa gggttttcct
    18001 accttgtttt caagccaggg tattaaatca tgctcatgat gagaatgatt cagtgcgctg
    18061 aattgaaatc gtggcgatac ttttggatga gataagacct gattccagtc aatagttggc
    18121 atgtggtgca gtttagccag agcatccatg gattttctga tgacattaaa attaatccgc
    18181 catttttcaa tataaggcaa ggcttctcta attgctctaa gcagttcaat aaaagtattt
    18241 ctggcttttg gattgtagtt ctcggtggct tctttaagat gttgaactgc tggaaaagta
    18301 tacttgccat ttctcattgc ttctaaatga aatactgatt gtaatgattt tagcaataca
    18361 gaaaaagggt cttccatacc aatattcctc cacaggccct tctttaaaag tagtttataa
    18421 aaacataaaa gccaaatatt ttgcaaaatg gattaataat tcttatattg attgacttta
    18481 tttatcgtct ttataaaaac aattgatttt atttattaaa tgatttcacc taaatttaac
    18541 aatgaaaata gtgttaattt aacgaggaga aaacttatga ttacggtagg caataaattt
    18601 ccagagtttc aacttaaagc aacggtagct aatgatataa acaatgcatt tcaaatgatc
    18661 tctaatgaaa cgtatcgggg taaatggtta gtcgtatttt tttggcctaa agattttacc
    18721 tttgtctgtc ctactgaaat tgcggagttt ggaaggctaa attccgagtt tgccgatcga
    18781 gatgctcaaa ttctgggtgg aagtacagac agtgaatttg tacatttggc ctggagaaat
    18841 cagcatccgg acttaaaaga tttgccattt cccatgctgg ctgatgttaa acgagaattg
    18901 acagcaaatt tgggcatatt ggatgacgag gagggggttg ctcaacgagc gacatttatt
    18961 gtagatcccc agggaattac tcgttttgtg atggttacgg atttaaatgt agggcgcaat
    19021 ccagctgaag tattacgtgt tctggatgct ttacagaccg atgagctttg tccatgtaat
    19081 tggaaaaaag gtgaagaaac aattcatatc aaataaattg aaaaaagagg catcaatcat
    19141 gttagagaat gttaaagaaa gtataccgga agtggctaaa gacatacgct tgaatttgac
    19201 taaggtactt gatttggaac aaagtgatgg attgagtgag catcagattt taggttctgc
    19261 attggctgtg agttattctt taggcaatga ggttttaatt agaggactta aagacttggt
    19321 gcataacgaa cagattgtgc gtgcagcaca acttgcggca agccttatgg cgatgactaa
    19381 tatttactat cgctttgttc atttaactga aattaaggaa attgagcata ttcctgcagg
    19441 attgcgaatg caaggaatga tgactccagg agtagacagg gtaacttttg aaatattgtc
    19501 ccttgctgtt tctgctctca atggttgtgg ggcttgtgtc agctctcacg cacgtcaatt
    19561 gaaagagcac ggcttatccg atacaagcct tgcacgagtt ggacgtatta ctgctgtgat
    19621 tcatgcggtt catgtgagtg gtcgaatcaa ataagtcatc gcatttgaag cagattatct
    19681 gcttcaaatg caaaaaacta tttaatgacg ccgcaagcta tacggtcccc cccgccacct
    19741 tgtggaggat tgtcactgta agtgtctcca ttggcatgga tcatgacagc taaattgtgc
    19801 atatcactta atttcaaacg aggagctaaa gtaggaatca ttgctttccc attacttgtg
    19861 acatataaaa caggtaaatc tcccaaatgc ccgttaccat aagggccttg gtggctattg
    19921 gtattttgag gatcataatg tccttctgca tccattccgt ggttaccaca atctgcattt
    19981 ttatgtaaat ggaaaccatg cggaccttca ggaaggttag ttaagtctgg tgtaatcaaa
    20041 acaccataag gggtttgtgt gaaggttacc ttgcctattg caactggttt gggaccagtt
    20101 gtgtaaattg gtgctgtaag atcatcggca atagccatgc ttgagaataa aatagtacta
    20161 attaatatta ttcctgattt attcatgtca tttcctggta tttttcaaag tccgttaata
    20221 tttttagtat tatccaagaa aaacatttat tccacaggat ttaaaaaaaa ttcgattttt
    20281 ttgggttaaa aatattttta attcagacgt ttagtatatc aggatttgca cgttcaaacc
    20341 ttcattgtgt agcccatgag agtttcgtaa actctatttt aaagtttaga gagaggatta
    20401 caaattaatt gaatcggtaa aaaatgattg ctgtataata agcaaggttt atactttatg
    20461 ttttaagctt taattaaaca gagagtcctc atggaattga gaattgataa tacccctttt
    20521 aaagccattt ttcagccttt ggatttgggc tttacccaaa ttaagaatcg tttattgatg
    20581 ggttcaatgc acactggact tgaggaagat aaagaaaatt taaatcgcct ggcaagcttt
    20641 tatagagaaa gggctcaggg tggagttgga ttaatcgtaa cgggtggttt tgctcctaac
    20701 cgagctggcc gtctggctcc cttcgctgcc aagctgacta atagtaaaga gcaacatcgg
    20761 catgagatca ttactcaagc ggtccatgaa gcaggtggaa aaattgcctt gcaaatattg
    20821 catgcagggc gttatggtta tcaccctttt attgtagcac caagcggcat taaatccccc
    20881 attagcccgt ttaaaccttg ggaaatgagt aaaagacgaa ttatcaaaac catcaagcat
    20941 tttgcccgat gtgccagact ggcccaattg gctggctatg atggggttga gatcatggga
    21001 agcgaaggat atttgataaa ccagtttata gtcactcata ccaatcatcg aagggatgaa
    21061 tggggaggaa gtttctccaa tcgcattcga tttcctattg aaatagttcg tagtgttcgt
    21121 gaggcggtag gcgagcactt tattatcatt tatcgcttgt ctatgcttga tttagtggct
    21181 gaggggagta attggcagga agtggttacc ctcgctaaag ccattgagca agctggtgcc
    21241 agtttaatca atacgggcat aggttggcat gaagcaagaa ttccaacgat aggaacgatg
    21301 gttccatctg ctgccttcac tcaagtgacc cagcatttaa agccggaaat taaaatacct
    21361 gtaattacat ctaaccgaat taatacacca gaattggcta atcaattatt agaagatggg
    21421 gtcgcagata tgatctctat ggccagacca tttctagcgg acccctgctt tgcagaaaaa
    21481 gcaaaattag gcgaaagcaa agcgattaat gtatgcatcg cctgtaatca agcatgcctt
    21541 gatcaagttt ttgtgaacaa aacagcatct tgcctggtta atccccgcgc atgcaacgag
    21601 acaaccttgg tttatgaaac aacaaatcaa ccgaaagcta ttgctgtagt tggttctggg
    21661 ccagctggcc ttgcttttgc tgtaacagca gctgagcgag ggcataaaat tactctattt
    21721 gaaaaagcaa atcagattgg cgggcagttt aatttagcga aaaaaattcc tggaaaagaa
    21781 gagtttcagc ataccttgaa ttattttgct taccagctcc aaaagtataa agtggatgta
    21841 cgtttgaata ctgaagctac tgtggaatta cttaaagact atgatgaagt ggtcttggcc
    21901 agcggaatta tacccagagt tcccgaaatt aaaggcatag atcataaaaa agtggtgagt
    21961 tatattgatg tgattactca aaataaaccc gttggctcac gagttgctgt cattggggca
    22021 ggaggaattg gttttgatgt cgctgagttt ttaactcatg atcatcaatt gacttctgag
    22081 caattttata atgaatgggg aattgatatt agcgggcagc atagaggtgg attaaaacca
    22141 cctgaaattg caccgagccc tcgtgaagtg tatttattac aacgcaaaaa agaaaaaatg
    22201 ggtaaacgat tgggaaaaac taccggatgg attcatcgat ccagtttaaa acataaacaa
    22261 gtcaaaatgt tttcgggtgt aaattacgag cgtattgatg atcagggatt gcacttgact
    22321 attgacgaca agcctcaagt tttggaagtt gattctatag tgatttgtgc aggccaaata
    22381 gaattaaaga atttgtatga gcctctgaaa atggcgggca agttggttca tttaatagga
    22441 ggcgcttata aagctttgga gcttgacgca cgtcatgcta tagatcaggc ttgtcggttg
    22501 gctgcattag tatgagtaaa attgctgtag tgataaatcc tatagcaggc ggcggtcgcg
    22561 gaaaaaaaat ttggaaagtc ctaaaacccg ggttacatgc cctattcgaa caaactgttt
    22621 acagaatatc taacagggtt aatgatcttg aagcaatcac agacggactt cttgctgaaa
    22681 accctgacag cctgttaata atcggtggag atggtacttt aaatcatgtt ttgaatggct
    22741 taattgagaa cgataaatta aaaaatccgc aaataaaaat cgcctttttt aacgcaggtt
    22801 gtggcggtga ttttacaaga caatttcccc aacagaaaat gaccgaattc ctggacagat
    22861 taagtcataa ccaatttatc aagtgtgata ttgggaaaat tactcttgcc aatcaagtgt
    22921 cgaggtattt tattaatatt gccagctgtg gtctgtcagg acatgtggtt ttgcgcgtat
    22981 ctaaaagcaa gtggttaaaa aaactgggcg gcactctgaa ttacttgttg catgctttaa
    23041 ccgggttgat gacctatcgt aaaaccaaag taagggttca gatagacgat gctctaccat
    23101 ttgatagctc catcctgctc atggccgtct gcagtggcca gtatttcggc ggcggtatgc
    23161 atgtagcacc aattgcaaaa gtgaacgatg gtttgtttga tgtggtttct tttaatgaat
    23221 tttcaaaact aaatgcactt ttaaagttat ataaaattta ttcagggggg catttgttgg
    23281 ataataatgt tcattatgtt caggctaaaa aaattaaaat agagccgttg gaagaaagta
    23341 aaatagaaat tgaggcagat ggtgaaatta ttggttgctt accggcacag tttgagttaa
    23401 ttaaagaaac tcttcctctt attgtttaaa aataaaagcc aaaatagggc tcattctata
    23461 aaattattat ttatcaagac aggttgttgc ctcaagacga gatcacatta ttaagtgcaa
    23521 taaacaacac aaacaattgt tcatattttg tcatcctgcg caggcgggaa gccattttta
    23581 ttgtaagctg tggagcaaaa tttaaaatag attttcactt gcgtgggaat gacacatatg
//
LOCUS       DAXLDT010000042        23087 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXLDT010000042 DAXLDT010000000
VERSION     DAXLDT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23087
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.042"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            246..1376
                     /locus_tag="ACF2SP_001274"
     CDS             246..1376
                     /locus_tag="ACF2SP_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HFD2359067.1"
                     /translation="MKEKYIGLHKIELNTPCLVIDYEKLKYNLKMMQEHGFKWGIGIR
                     PHCKTHKCTQIARLQLEYGAIGISAAKLSEAEVLVSKQIANVLITSPIVSEAKHSRLF
                     NCLKSAPDLMLVVDGKDNIRMLNELGKSLNQPVYVLLDIDSGIGRTGINPEAALEYGH
                     FINGMNWLKLKGIQCYAGHLQHIQNYEERKAFSLQIMEMASRIVQSFRDANLPCEILT
                     GTGTGTFDIDVEASEVTEIQPGSYAVMDVEYGSIGSKNNPSSFRLFKNSMTLLTSVIS
                     SNRREHATVDAGTKSIYVDPHHKPHIISHPGLVYDWGGFGDEHGKVFSCEGGSHFLQN
                     GDVLELIVPHCDPTINLYDKFYITENNVVVDIWDIDMRGKSQ"
     gene            complement(1417..1596)
                     /locus_tag="ACF2SP_001275"
     CDS             complement(1417..1596)
                     /locus_tag="ACF2SP_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HFD2359068.1"
                     /translation="MLTWALIFFIIAIIAAAFGFGGIAVAAAGIAKILFFLFLVMFVI
                     FLIMGLVGRRGPPPV"
     gene            1818..2186
                     /locus_tag="ACF2SP_001276"
     CDS             1818..2186
                     /locus_tag="ACF2SP_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359069.1"
                     /translation="MQTTKAHYSKIFFALFLFFCTGSSALFACPCFNMGFLHVAFLDD
                     KDTECRIYKEGSIIYKIEIFDKEKIASSTHTQCTLNTEHHDVFMNYDFHYLDDHGDCI
                     REILDACNKLKINSLHMDDE"
     gene            complement(2279..2809)
                     /locus_tag="ACF2SP_001277"
     CDS             complement(2279..2809)
                     /locus_tag="ACF2SP_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359070.1"
                     /translation="MSKFPHKTPETLRQYFREKSLEELIEINRSYGPHFENLENTIDR
                     LTQEISTRAERLPSLLKRLEELAQNYGKIAIEQERYEDNRASIMSDSCTGAERYLALS
                     ALGMSPFDSYHHNSRNLQNEIDTINKKLNELNSHLALWKNQRSKAVSELKILNPIIDE
                     KRRELEIALQFTLSSN"
     gene            complement(3029..3370)
                     /locus_tag="ACF2SP_001278"
     CDS             complement(3029..3370)
                     /locus_tag="ACF2SP_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359071.1"
                     /translation="MSMTLSLGQKYGSFFWGCTKFAVSPYTNTFLSIVKSKEVEEADC
                     LSKALAASIVCGVLTFVVPVLPVLCAFTFTLSSIAMLLAVASMFVTYPIAILGDALCG
                     PELSDDYRYSF"
     gene            complement(3567..4040)
                     /locus_tag="ACF2SP_001279"
     CDS             complement(3567..4040)
                     /locus_tag="ACF2SP_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFD2359072.1"
                     /translation="MMIDRTDKKILEILQANCEINNQELADLVALSPSSCLRRVKLLI
                     DNGYIKKQVALLEPEKVGLKLTVIVLVGLNNHQPEVMNQFEKTIQLFPEVIQCYLITG
                     QSADYLLKIIVPDLNAYQAFLLGKLTRINGVASVHSSFILRNVSDTTALPLDHLD"
     gene            4240..5472
                     /locus_tag="ACF2SP_001280"
     CDS             4240..5472
                     /locus_tag="ACF2SP_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359073.1"
                     /translation="MSVSRVTAWFVWVIVSIFYAYQYVLRVMPNIMLDDITQQFHIDA
                     IIFGQFSGIYYIGYSLMHLPIGIMLDRFGPRKVMTGCIMLTVIGLLPILFAENWVYPV
                     IGRALIGIGSSAAILGTFKIIRMTFKEKHFPRMLSFSVMIGLVGAVYGGGPVSYMCQL
                     MGYKMVVEIFVGIGIVLAGITYLIVPDVQPSRQDSVISNIRMVFGNSKVILLCCFAGL
                     MLGPLEGFSDVWGSGFLKMAYSLNTNAANYLPSMIYIGMCFGAPVLSLIAEKTGYYLG
                     TIIASGVVMFLVFAMLVGGLLTVPSITISFILVGICCSYQILAIYKVSTYVPDNLAGL
                     TTAIANMIIMIFGYGFHSVIGYVVNMYGGTHVARAFVYGVGVIPLTLAVGVVGFLFLA
                     YQEKNFTPDASFASPELR"
     gene            5527..5841
                     /locus_tag="ACF2SP_001281"
     CDS             5527..5841
                     /locus_tag="ACF2SP_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFD2359074.1"
                     /translation="MYMLYFHVPETHLDEVKNAVFAAGAGYVDNYNHCAWQTLGEGQF
                     MPLPGGQAFIGEINQLEKVPEYKVETICARERIKEVVAALKKAHPYESPSYQVVRVET
                     EF"
     gene            6032..7585
                     /locus_tag="ACF2SP_001282"
     CDS             6032..7585
                     /locus_tag="ACF2SP_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFD2359075.1"
                     /translation="MLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSA
                     FQNANWWKVFKDPTLTALIDNGYHNNLSLQMAGVRVLQARAQLAQSVGELYPQQQAMV
                     GDYNYNRVGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAY
                     DSALVTLTSDIASTFIKIRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQ
                     AATELAETQSKLPQYVSDLQHQKDILAVLLGTTPDKVDDLLKKNKGIPKASAVVEVGI
                     PKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGTFVFASNNIGNSTISDIF
                     NWSNRNIVAGPSLTWPLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITR
                     YVEGQKTVSSLKKANASAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATG
                     ELALSLVGLYRSLGGGWQIRGKNDIVSNQIKSEMAARTNWGSLLKQENHLPPDSNGQR
                     MKQLYLPTW"
     gene            7591..8745
                     /locus_tag="ACF2SP_001283"
     CDS             7591..8745
                     /locus_tag="ACF2SP_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2359076.1"
                     /translation="MNSQNKQNLVKTVAIVFFGIVFVYLVYDHFTRTQVATPAPPVVI
                     VQKPKLMKMGQYVTQTGNTVAFQSVDLVARIEGYLDAIKFTDGAFVKKNQELFIIEPE
                     PYLEQLRAAEAKVAAQKANYAYAKSEYARQQRMYKQNATSLNSVEKWKAKVEEAQAEV
                     DKSEADATNAAITYGYTHISAPFDGRMGRHLVDVGNLVGHGAATDLATIEQIDPIYVY
                     FNLNELDLIRIRNAARKRGYKPMELVKIPVEVALQDEKGFPHKGTLDFVNTGLNASTG
                     TMEFRALLPNKGYPLVPGLFVQVRVPISEPSPKLTIPDTSVQYDQIGPYVLVVDKDNY
                     VLTKRVTLGGLENGVRAITKGLDAQDSVIVSGLQNATPNHQVTPKTEQPE"
     gene            8805..11957
                     /locus_tag="ACF2SP_001284"
     CDS             8805..11957
                     /locus_tag="ACF2SP_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2359077.1"
                     /translation="MISKFFIERPVLANVIALFIVFIGIIAIAVLPVSQYPAIVPPTI
                     QVTTTYPGADAKTLINTVALPIEQQVNGVEDMLYMQSTSTSGGTYTLIVTFAIGTDLN
                     YAQVLVQNRVQAAMAQLPESVQKQGVVVQQKSTAILQFITLTSKNNEYDGVFLDSYAT
                     INMQDELSRLPGVGNVVVFGSGSYAMRVWLDPKKMLAFSLNPSDVLNAISYQNKEVSA
                     GQLGAPPTVGKQAYQFTVNVPGQLSDPKEFENIIIKTIDTPPDQNSSASSSAQVVRIR
                     DVGRVELGSESYNQLANLNGKPTAAIGIFQLPGANALDVAQEVRKAVAKMAKQFPPGL
                     EYSIPFDTTVFVKASIEEVYKTLFEAGVLVLIVIIVFLQNFRASLVPATTVPVTIIGT
                     FFAMLLLGYSINLLTLFALVLAIGIVVDDAIVIVEGVTQHIEKGMSPKESAILAMKEL
                     FGPIIGITLVLMAVFVPAGFMPGLTGSMYAQFALVIAATAFISAINAMTLKPTQCALW
                     LKAPDTSKPKNIFFQAFDRIYNPIESAYVKFIDRLVHRSGKVCLIGIILVACAIYGLT
                     RIPTGFIPMEDQGYLMLSIQLPDGASLGRTDEVVSRLAKKASEVGGVDNVIAIDGISL
                     LDNNSLLSNAGVIYVIFKDWSVRGKSENLRALYTKFNAIAKETLDAKVLVVVPPPIQG
                     LGMSGGFQMQVELQDGTFDYRKLQQATDQMINTGRQYPQLQNLMTTFRASVPQVAAPI
                     NRTKAESLGVRVADAFDTLQTYLGSSYVNLFTKFGQVFPVYVQADASSRISSEDLRNY
                     YVRNQSGSMVPLGTLTDVGPAVGPSIISLYNLYPSSNINGVAARGYSSGQGIQVMEEL
                     AKEQLPPGISYEWTSTAYQEKVAGNLSYFIFALSLVLVYLILSGQYENWLIPSAIILS
                     VPLTLVGTVLALGSLGMDNNMYTQIGLLLLIALATKNAILIVEVAREQREIHNKSVLE
                     AAVIGAKTRFRPILMTSFAFIMGVMPLVFATGAGANSRRSIGIAVSSGMLASTCLAVV
                     FVPVFYVLLQTWQDKRKAKH"
     gene            complement(12276..15002)
                     /locus_tag="ACF2SP_001285"
     CDS             complement(12276..15002)
                     /locus_tag="ACF2SP_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SdcA"
                     /protein_id="HFD2359078.1"
                     /translation="MVINMVDVIKFKEPERCDYLYIDENNKVHILLPIVGGDEIGLDN
                     TCQTAVELITFFYGSAHSGVTKYSAEHQLSEYKRQLEEDIKAINSQKKISPHAYDDLL
                     KEKTERLQQIEKYIELIQVLKKQYDEQNDIRQLRTGGIPQLPSGVKEIIKSSENAFAV
                     RLSPYDNDKFTRFDDPLFNVKRNISKYDTPSRQAPIPIYEGLGYRLRSTLFPEDKTPT
                     PINKKSSKEKIVETVLAQVDADYMKDGDKEQKLEELKKLIQQELVKIDSNLSVNMSRD
                     GKEVNLSYLDIVMALDDDSTIKDWIEGIIEVSIDPTVWEAQASSPFYDGAKEIKSDHE
                     ADRISIRVQYLLAEANIYCKTNKLSDANFGEFFDKEPHATEIAKRVKEGFAQGAEIEP
                     IIYDYINSNHAELGLKSPLTGKQQQEITEKFTRHYNTIKESPHFDEFFVADPDKKGNI
                     FSHQGRISCHFLDFFTRQTKGKHPLGDLASHQEALQEGTSNRLHHKNEVVAQGYEKLD
                     QFKKEVVKLLAENKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRNWPAIQKELE
                     KATSIPESQKQDLLRLLSRDNLQHDNLSAITWSKYSSKPLLDVELNKIAEGLELTAKI
                     YNEKRGREWWFKGSRNEARKTQCEELQRVSKEINTLMQSESLTKSQVLEKVLNSIETL
                     DKIDRDISAESNWFQSTLQKEVRLFRDQLKDICQLDKYAFKSTKLDEIISLEMEEQFQ
                     KIQDPAVQQIVRDLPSHCHNDEAIEFFKTLNPEEAAKVASYLSLEYREINKSTDKKTL
                     LEQDIPRLFKEVNTQLLSKLKEEKAIDEQVHEKLSQLADKIPPEHFTRNNIIKWSTNP
                     EKLEESNLNEPIKSVQSPTTKQTSKQFREAMGEITGRNEPPTDTLYTGVIKK"
     gene            complement(15153..17831)
                     /locus_tag="ACF2SP_001286"
     CDS             complement(15153..17831)
                     /locus_tag="ACF2SP_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SdcA"
                     /protein_id="HFD2359079.1"
                     /translation="MADVIKFKEPERCDYLYIDENNKVHLLMPIVGGDEIGLDNTCQT
                     AVELRSFFYGNTHHGEARHSAEQQLTDYKKQLEEDIKAINSQKGISRYAYTDLLREKK
                     ERLKQIEKYIELINVLKTEYDHNGEIMTIKNNIIPPLPSGLNQIIQSSENAGAVRLSP
                     DRPDLATSFKNPLFRLNRHYETSDYKLTEGLGVRLSSTLLPDPETPTPINRKSQKEKI
                     VETVLAKFQPEKIAEPDRDQKLKELKALLQEELVKIDSNLSVDISHDKQETNYDYLEN
                     MMGMDEDSSIQEWVDSILTATVDSSVWVTQSASPFYDGAKEIKQKDDADKMSIRVQYL
                     LAEANFYCKTNKLSDANFGEFFDKEPHATEIAKRVKEGLVQGVDIEPIIYNYINSNHA
                     ELGLKSPLTAKQQQEITDKFSQHYNTIKDSPHFDEFFIADPDKKGNIFTHQGRLSCHF
                     LDFFARQTNAKHLLGELDGHAEALLEGTSNRLNHKNEIVAEGYEKIEQFKQEVVRLLA
                     ENKPKELLDYLTATSPTGVPNYSLLSLETQNYISYNRNWPAIERELQKSDNIQPNIKQ
                     DLLRLLSRDNVQHDNLSAITWSKYSSKPLLEVELSKVAEGLNLTADIYDEKRQQQWYK
                     GSRNEAREAQCAELKKVAEEINTLLDNPSLSKGEVLNTLLKSIETLDKIDDEISSEFN
                     LFQSTLQKEVRLFREQLKDICQLDNYAFKSTKLGEIISLEMEEQFQKIKDPTVQQIVR
                     DLPSHCHNDEAIEFFKTLNPEEAAKVASYLSLEYREINKSTDKDKLLNEDIPNLFKEV
                     NMQLLSKLKEDSVLGEGVYEKLAQLADKIPPEHFTRNNIRKWSANPEKLEESNLGELL
                     KSSDGSLSEMARKYRETINEMAGRNEPPRETVRQTI"
     gene            18090..19283
                     /gene="dupB"
                     /locus_tag="ACF2SP_001287"
                     /gene_synonym="laiF"
     CDS             18090..19283
                     /gene="dupB"
                     /locus_tag="ACF2SP_001287"
                     /gene_synonym="laiF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupB/LaiF"
                     /protein_id="HFD2359080.1"
                     /translation="MLYNKSSFCLWKETIVPIILDSDVLEVAEYVYKTRLSQPYTEVG
                     SEWEYNYKNPTATFAKGDGHNLQRYITIDGKQLHRPIHGLAHTMRTLMYSQLMYCSSK
                     KQLSPHVCQDGRTIADLSELDLKKINIAQLFFVAGRESEASYGDAYHRYHLYGAKQFE
                     EYARKHLTHLFSEEEIILYSRCIEDRVGDSFDGTPEGYIIHLSHMIDLMRCKSPVEVF
                     LGHSKGVSGIVPTLIHLFGKQDGLDIMHYARGLFAATGEAVPYIDSSEWPHLGVDLSR
                     VQRALSIVGDINVPGQEADSKKTAQAGFSVDGCYSALTSVPTPSWYEKELKEVDDEKI
                     DVKEVDDREIEKEHEIVVPSQATTPLSTEKPGSFVEKFLKPFMIWKKPEVQTTQPTTE
                     KTNKP"
     gene            19355..21778
                     /gene="sdjA"
                     /locus_tag="ACF2SP_001288"
     CDS             19355..21778
                     /gene="sdjA"
                     /locus_tag="ACF2SP_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdjA"
                     /protein_id="HFD2359081.1"
                     /translation="MFSYLDKLLDGIFGNQTPDTSQLSTSKSPTLTTVPLKQEYFVKI
                     GESETQDLGLLPVVSKRHNQSVPLEEIPFEETRLELIGLYITLLKQCVLDEKLKSIPA
                     QYLISHYLFIKTLAANEGNKGRKDLYLNLAQKVADYLEKNESKIWSMAVECTKTSEYP
                     IVDWIKKHHLHFNFIRAFILDYNKKPLTHNQRAFMQQFRDSAAFFFPDQVYLAWLTQS
                     YESGSILNPMYRESRSTHYYHANITDNLLLRTRPKQVNLGPQHFFQKGKGSVKNTYRF
                     NINDGKLMRIQGRTLLFSTNKGNEVIAVKVQKKGEPQSALSNEFQMADYLLKHQRRLN
                     LQSQLPTPLSQYSINRTEIIEKCSKSPDFEKFKNLISDAKSLEIYVYKATPSYFTYLH
                     DKQQSFSQLTSSVQKNVHDLFMLLREGIVFPYLADMFHTHIDESKRSDKGRYQTLVEL
                     LSALQSQMGRLDKWQKAVEFVNLRASGIADLGDNLPLISFLTVSDWTKHYHAELLTGV
                     YHPSFLFLDKSSGSVRSLFNSRRKVFGNYLYLNIIAEYLLVIQLVIGCYGDKVTRKMD
                     SKSKAKVWEHLAELMFSSCAEAVNLITGMPKSRALAFLKQRANVRKHTQQTSFWMTPD
                     YSNLDRLSVQSQQSTLYPGESDYEINSDLIPGVGLSLDGINQDLGDYNQASPLRELEK
                     LLYATVTLIEGTQQLDKQFFQQLDETEKMILSAAGVDKCYQAVAKLLDLARPGCQMQR
                     RLAFTYYEMIKRIYPCSNPADVRFELVAKEEAIIKIQRFWREHKKENQSIEKGFDFDR
                     NTSSSQSPL"
     gene            22175..22870
                     /locus_tag="ACF2SP_001289"
     CDS             22175..22870
                     /locus_tag="ACF2SP_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HFD2359082.1"
                     /translation="MPEETSTNLPKSLSHFLIQSDLDYKNSLSSIPLFDVKRTIFWDD
                     NLRKMFASIFYHLRGHFINFLWYVANFADNEASKQIIIQNIYEEIGIGNRFSHEKLYE
                     RFAKECGVDIHDEIVNETNYIPFAKEFNKAHLSWLAQHDPIDHLCAFAAYERLDNLDY
                     PFLVELAKSMKISQHGMTFFNVHTHVDHFDTTLQLIVPIWEESPQRLIESFHFIQTHQ
                     LGMWEQFSNVLFN"
     gene            complement(22918..>23087)
                     /locus_tag="ACF2SP_001290"
     CDS             complement(22918..>23087)
                     /locus_tag="ACF2SP_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD2359083.1"
                     /translation="EYEKIFDFFYSGRKEGTGLGLAYCKLLMQAAGGDISCKGKKNEF
                     AHFTLKFPKID"
BASE COUNT         6721 a         4108 c         4649 g         7609 t
ORIGIN      
        1 gatgttttag ttttactgta ccactttaag tataatggca cagcaataga ctctgcagga
       61 gttatatttt caagtttgaa cataggttaa attactacct tttgaagctt tattaaaact
      121 catgatgccg ttggaggggc aaatgtctaa tgcctcaagt gatctaagta aagatttaaa
      181 ttgtggcaaa cctaaataat ttatgtcata aaacgcattt attttacatt gtgatgaatt
      241 atctcatgaa agaaaaatac atcgggctcc ataagataga actaaatacc ccatgccttg
      301 tgattgatta tgaaaagctg aaatacaact taaaaatgat gcaagaacat ggtttcaaat
      361 ggggcatagg aataaggccg cattgtaaaa ctcataaatg tactcaaatc gcacgtttac
      421 aacttgaata tggggcaata gggatttctg ccgccaaact atcagaagct gaagtattgg
      481 tatccaaaca aatcgcaaat gtcttaatca cttcacccat tgtatcggag gctaagcatt
      541 caagattatt caattgtctt aaatcagcgc ctgatttaat gctggttgta gatggcaagg
      601 ataatattcg catgcttaat gagcttggaa agtcgctaaa ccagccagtt tatgtcttgc
      661 ttgatatcga ttcaggtatt ggtcgaacgg gcattaatcc cgaggcagca ttggaatatg
      721 gtcatttcat caatgggatg aattggctta agctaaaagg gattcaatgt tatgcaggcc
      781 acttgcaaca tattcaaaac tatgaagaga gaaaagcgtt ttctctacag attatggaaa
      841 tggccagcag gattgttcag agttttagag atgccaatct tccttgtgaa atattaactg
      901 gcaccggaac tggtactttt gatattgatg tcgaagcaag tgaagtaaca gaaattcagc
      961 ctggctcata tgctgtaatg gatgttgagt atggttcaat tggttcaaaa aataacccat
     1021 caagctttcg gctatttaaa aactcgatga ctcttttaac gagcgtgata agcagtaatc
     1081 gtagagagca tgccactgtt gatgcaggaa cgaaatcgat ttatgtcgat cctcatcata
     1141 aaccacatat tattagtcat cccggactag tttatgattg gggtgggttt ggagatgagc
     1201 atggcaaggt attttcttgt gaaggcggaa gtcatttcct gcaaaatggc gatgttttag
     1261 agcttatcgt tcctcattgt gatccgacaa taaatttgta tgataagttt tatatcacag
     1321 aaaataatgt tgttgtcgat atttgggata tcgacatgcg tggaaaatcg caataatcta
     1381 tgctattcaa ccaggtgttt gagcttcata tttctgctaa acaggaggag ggccacgacg
     1441 tccgactaat cccataataa gaaatatgac aaacatcacc aaaaatagaa agaataatat
     1501 ttttgcaatt ccagcggcag ccactgcaat accaccaaat ccaaacgcgg ctgcaataat
     1561 tgcaatgata aaaaaaataa gtgcccaagt taacataata cctcctgtag gacttacgaa
     1621 aaaccccaag gtcaaggcaa aaaaatgttt ttaatgaggg agtttagaat aaactaaatg
     1681 accgaattaa aaacattttt ttaacgacga gattggggct ttacgtaagt cctgtccctg
     1741 tcaagattat ccttttgtct aattgtagac caataatggt ctacaattag acaaaaggca
     1801 gttaatcagg gaagaatatg caaacgacta aagctcacta tagcaagata ttctttgcct
     1861 tatttctttt tttctgcact ggttcaagcg cattatttgc ctgtccttgt tttaacatgg
     1921 gctttttaca tgtagccttt ttagatgata aagatacgga atgtcgtata tataaagaag
     1981 gaagtattat ctataagata gagatttttg ataaggaaaa aattgcgtca agcactcata
     2041 cccaatgtac gctgaataca gagcatcatg atgtgtttat gaattatgat tttcattatc
     2101 ttgatgatca tggagattgc atcagggaaa ttctggatgc gtgtaacaag ctgaaaataa
     2161 attccttgca tatggatgat gagtaagata aagcaaggtg aaataggact ggtattgaat
     2221 gattaggacg ctcacccgga ttataattaa gattaacccg ggttagcctt atcaattttt
     2281 agtttgaact caatgtaaac tgaagggcga tttcgagttc tcttcttttt tcatcgataa
     2341 tggggtttaa tatctttaat tcagaaaccg ctttcgatct ttgatttttc cacaaagcaa
     2401 gatgagaatt taattcattt aattttttgt ttatcgtatc gatttcattt tgtaaattcc
     2461 tgctattgtg atggtaggaa tcgaaagggg acattccaag agcggagaga gctaaatagc
     2521 gttctgctcc ggtacatgag tcactcataa tcgatgcgcg attgtcttca tatcgttctt
     2581 gttcaatagc gatttttcca taattttgcg caagctcttc cagtcttttt aaaagactgg
     2641 gtagtctttc tgcacgagtg gatatctctt gagtcagtct atcaatcgtg ttttcgaggt
     2701 tttcgaaatg aggcccatag gaacgattaa tttctattaa ttcttccagg cttttttctc
     2761 ggaaatattg acgaagtgtc tcgggggttt tgtgcgggaa tttagacatg tttttacctt
     2821 tttgaatcaa aatcagcata tcttagcata tgctttttga tcatatcaaa catatatgct
     2881 tcataatgag taaattaagg gcttttattg atgactgggt tcatttttgc tcaatatcaa
     2941 acaggaagag tatttttacg agtttaaaaa tactcttcct gttggttgat acttatgatg
     3001 atttcaaatg aaattatctt aaaactgatt agaaagaata acggtaatcg tcagacaatt
     3061 ctggtccgca cagtgcatca ccaagtattg cgatcggata agtcacgaac atactggcta
     3121 ccgcaagcaa catggctatt gaagacaggg taaacgtaaa agcacataga actggtaata
     3181 ctggaacaac aaaagtgaga actccgcaga caatgctggc agcaagagcc ttggataaac
     3241 aatctgcttc ttcaacctct ttcgatttga cgatggatag aaaagtattg gtataggggg
     3301 aaacggcaaa tttagtacat ccccaaaaga atgagccgta tttttgacct aacgagagag
     3361 tcattgacat tttttactcc aatttaactt aaattaggtc agcaattata attcaaattc
     3421 agattaaaac ctataaaaat aagcattaaa ttaataaatt tgttctattt ttatctattt
     3481 tttatagaat ttttgatttt ataggtagag ccgggtactt tcgagttagt atttattgaa
     3541 taatttaata ttcgaagaac agtgtcctaa tccaaatgat caaggggtag ggcggttgtg
     3601 tcagaaacat tgcgtaatat aaagctggag tgaacacttg caacgccatt gatgcgtgtt
     3661 aatttgccta ataaaaaagc ttgataagca tttaaatcag gcacaattat cttcaataaa
     3721 taatcagcag attggcctgt aatcaagtag cattgaatga cttccggaaa aagttgtatg
     3781 gttttttcaa attgattcat aacctcaggc tggtgattgt ttaaccctac taaaacaata
     3841 acggtcaatt ttaaccctac tttttctggc tcaagcaggg caacctgttt cttgatataa
     3901 ccattatcta ttaataattt gactcgacgc aaacaggaag aaggagacaa ggccacaagg
     3961 tcggccaatt cctggttatt gatttcgcaa tttgcttgca gtatttcaag tattttttta
     4021 tcagtacgat cgatcatcat attttatgca atgaaaatgg cattgatatt tcttaatata
     4081 gaattatatt ccaaaaaaat ctattaaatt agaatttaaa atcaaaaaag agggttttat
     4141 tttgtttttt gcaatccaat ttcaagggaa gtaagataaa atactttatg atgattattt
     4201 gatctccgtg gtcttttatt tgcttttctg agggtgatta tgtctgtttc acgagttaca
     4261 gcgtggtttg tgtgggtcat agtatccata ttttacgcat accaatatgt tctgagagtt
     4321 atgcccaata ttatgctgga tgatattact cagcaatttc acattgatgc cataattttt
     4381 gggcaatttt ctggtattta ttacattggt tattctttga tgcatttgcc tattgggatt
     4441 atgctcgatc gttttggccc aagaaaagtg atgacaggat gcatcatgtt gaccgtgata
     4501 ggtttacttc ccattttgtt tgctgaaaat tgggtttatc cagtgatagg aagagccctg
     4561 attggtatag gttcatcggc tgccatttta ggaacattta aaataatacg catgactttt
     4621 aaggagaaac attttccaag gatgctgagt ttttcagtca tgataggact tgttggcgcg
     4681 gtatatggtg gcggccctgt aagttatatg tgtcaactta tgggttataa aatggtggtg
     4741 gaaatatttg taggcatagg aatagtgctg gctggtatta cctatctgat tgttcccgat
     4801 gtacagcctt ctcgccagga tagcgtgatt tctaatatca gaatggtttt tggtaatagt
     4861 aaagtgatcc tgttgtgttg ttttgcggga ttgatgttgg gaccactgga aggtttttct
     4921 gatgtatggg gctctggctt tttaaaaatg gcatacagtt tgaataccaa cgcggcaaat
     4981 tatcttccat caatgattta tattggcatg tgttttggtg cccctgtctt aagtctgatt
     5041 gcagaaaaaa cagggtatta tcttggaact atcatcgcat caggcgtggt tatgtttctg
     5101 gtatttgcta tgcttgtcgg aggtcttctg actgttccca gtataacaat aagttttatc
     5161 ctggttggca tttgttgttc ttatcaaatt ttggctattt ataaagtatc cacttatgtc
     5221 cccgataatc ttgcagggtt aactactgca attgcaaata tgattattat gatttttggg
     5281 tatggttttc attctgtaat tggttatgtc gtcaatatgt atggggggac acatgtagct
     5341 cgcgcttttg tttatggagt tggggtgatt cctttgacat tggctgttgg cgtggtgggt
     5401 tttttgtttc tggcctatca agagaaaaat tttaccccgg atgcttcctt tgcctctcct
     5461 gaattacgat aattactggc atttctatct tgtgtggtaa taaatatcaa gggaggtata
     5521 gctatcatgt atatgttgta ttttcatgtt ccagaaactc atcttgacga agtaaaaaat
     5581 gcagtttttg cggcaggggc tggttatgtt gataattata accattgtgc ctggcaaact
     5641 ttaggtgaag gccagtttat gcctttacca ggtggtcagg catttattgg agaaattaat
     5701 caattggaaa aagtgcctga atacaaggtg gaaacgattt gtgccagaga gagaatcaaa
     5761 gaggtggttg cagctttaaa aaaagctcat ccctatgaaa gcccttctta tcaagtggta
     5821 cgtgttgaga cagagttcta aatattagag cgagaaattc aggcaggtcg aatcaccaga
     5881 tggttggtca attagtaaga atttgtttct tcaggatact tgttcaattg agtattcata
     5941 gataatatct aatgattgat gaaccaatag ttcaaatcca tcttcaatct acataagctg
     6001 cttttgaaca gatagggaaa tactggtata catgttgaaa atccttgctc tcttaagttg
     6061 tttgattcta agctcctgtc tggtcgggcc aaactttaaa gagcctaaaa aagatgcggc
     6121 caattattgg cttacttcaa gccctgcaat aaaacaatcc gcttttcaaa atgcgaattg
     6181 gtggaaagtc tttaaagatc ccactttaac cgcattaatc gataacggtt atcacaacaa
     6241 tctctccttg caaatggcag gagtacgagt tttgcaagca agagctcaac ttgctcaatc
     6301 ggttggggag ctttatcccc agcaacaagc tatggtggga gattataatt acaatcgagt
     6361 tgggggcaat caattacaag ggcttctacc ccccgatttt ttaacagcta gcctcggatt
     6421 ttccgcaagc tgggaacttg atttttgggg aaaataccga agagcgattc aatctaatga
     6481 tgcactgttc cttgcctctc tggccgctta tgattctgca ttggttactc tcacgtcaga
     6541 tattgccagt acttttatta aaatccgcac cagcgaagaa ttaatcaaag taacaaaaac
     6601 taatatccag gtgcaggcga tgagtcttaa aatcgcaaag gaccgttatt atggtggtca
     6661 aaccagttta ctggatgtgg aacaagcggc aacagaactt gcggaaactc aatcaaaact
     6721 gcctcaatac gtgagtgacc ttcaacacca aaaagatatt ttggctgtat tacttggtac
     6781 cactcccgat aaagtggatg atttgcttaa aaaaaataaa ggaataccaa aagcttcagc
     6841 ggtagtggaa gttggaattc ctaaagaagt gatagcgcga aggccggatg tttatcaagc
     6901 cagaatggag gctatagccc aatcagctgc aattggcgcc gtgaaggcta atctttatcc
     6961 ttcactttca ttagtgggga cttttgtttt tgcttcaaat aatattggca attctactat
     7021 tagtgatata tttaactggt ctaatcgcaa catagtcgca ggcccgtcac tgacatggcc
     7081 tttattaaat tatggtcaaa tcacgaacgc ggtaagggct caagatgctg ctttccagca
     7141 agcattgctt aactatctta atttggtgct caaagtgcag caggaagttc aggacaacat
     7201 tacgcgctat gttgaggggc aaaaaaccgt ctcttcactt aaaaaagcca atgcttctgc
     7261 catagaatcg acaagacttg ctttagtacg atataaggaa ggagaggcta attacaccac
     7321 tgtattgaat gcagaacagc aacaattacg agtacaaact tcactggtta atgccacagg
     7381 agaattggct ttgtctctgg ttggcttata ccgctcgctt ggaggcggtt ggcaaatacg
     7441 aggcaagaat gatattgttt caaatcaaat aaaaagtgaa atggctgccc gtactaattg
     7501 gggtagttta ttgaagcaag aaaatcattt gcctccagac tctaacggac aacgaatgaa
     7561 acaactatat ttaccaacct ggtgatgaga atgaactctc aaaataaaca aaatttagtt
     7621 aaaaccgtag cgattgtatt ttttggtatt gtctttgttt atttagtata tgaccatttt
     7681 acaagaaccc aggtagcaac cccagcacca ccagttgtga tcgttcaaaa gcccaaatta
     7741 atgaaaatgg gacagtacgt tacccagaca ggcaatacag tagcatttca atcggtagat
     7801 ttggtggcac ggattgaagg ttatttggat gccataaaat ttactgacgg cgcttttgtc
     7861 aaaaaaaatc aggaattatt tattattgaa cccgagcctt atcttgagca actgcgtgct
     7921 gctgaagcca aggtagcagc tcaaaaagca aattatgcct atgccaaatc agagtacgcc
     7981 agacagcaac gcatgtataa acaaaatgcc acgtctctta acagtgttga aaaatggaaa
     8041 gcaaaagttg aagaagcaca ggcggaagta gataaatcgg aagctgatgc cacaaacgcc
     8101 gcaattacat atggttatac ccacatttca gctcctttcg atgggcggat gggaaggcat
     8161 ttagtcgatg taggtaattt ggttgggcat ggtgccgcaa cggatttggc taccatagaa
     8221 caaatcgacc ctatttacgt ttattttaat ttaaatgaac tggatttaat caggattaga
     8281 aatgcagcgc gcaagagagg atataaacca atggaattgg tcaaaatccc tgttgaagtg
     8341 gctttgcaag atgaaaaagg atttcctcat aaggggacat tggattttgt aaatactggt
     8401 ttaaacgctt caactggaac catggagttt cgcgccttgt tacctaacaa gggatatccc
     8461 ctggtgcctg gtttatttgt gcaagtccgg gtaccgatat ctgagccgtc tccaaaatta
     8521 acaatacctg acacctctgt tcaatacgat caaattggcc cctatgtcct tgtggttgat
     8581 aaagacaatt atgtgcttac caaacgcgtg acacttggcg ggctagaaaa tggggtaagg
     8641 gctattacta aaggcctcga tgctcaagac agtgtgattg tttccgggtt gcaaaatgcg
     8701 acgcccaatc atcaggttac gcctaaaact gagcaacctg agtaagtatg gccaaaaaat
     8761 tgtaagaatt taagaccctt tattcaggga gcgaaacaac tgaaatgatt tccaaatttt
     8821 ttattgaaag acccgttctg gccaatgtca ttgccttgtt tattgtattt atagggataa
     8881 tagcgattgc tgttttgcct gtgtctcaat accctgccat tgttcctcct accatccagg
     8941 ttactacgac ctatcctggc gctgatgcaa aaactttgat taataccgtc gctttgccaa
     9001 ttgagcaaca agtcaatggg gttgaggaca tgctgtacat gcaatcgaca agtactagtg
     9061 gaggcaccta tactttaatt gtcacgtttg ctataggcac ggatctgaac tatgcgcaag
     9121 ttctggtgca aaatcgagta caagcggcga tggctcaact ccccgaatca gtacaaaagc
     9181 aaggtgttgt cgttcaacaa aagtcgactg ctattcttca atttattact ttgacttcaa
     9241 aaaataatga atacgacggc gtatttcttg acagctatgc aaccatcaat atgcaagatg
     9301 aattatcccg tttacccgga gtagggaatg tggtggtttt tggttcagga agttatgcga
     9361 tgcgtgtttg gctcgatcct aaaaagatgc ttgctttttc tttgaaccca agtgatgttt
     9421 taaatgctat cagctaccaa aacaaagagg tgtcagccgg acaactgggt gctcctccta
     9481 ctgttggcaa acaagcctat caatttacag tgaatgtgcc tggacaactc tctgatccca
     9541 aagagtttga aaacattatt attaaaacga ttgatacccc acccgatcaa aattcctctg
     9601 ccagtagctc tgctcaggta gtacgtattc gtgatgtagg gcgagttgag ttgggatccg
     9661 agagctataa tcagctcgct aatcttaatg gtaaacctac agcagcaata ggtatctttc
     9721 aacttcctgg tgccaatgcg ctggatgttg cgcaagaagt tcgtaaagcc gtggcgaaaa
     9781 tggcgaaaca gtttcctccg ggtcttgaat attctattcc atttgatacc accgttttcg
     9841 tcaaagcttc aattgaagaa gtatataaaa cgctttttga agcaggagtt ttggttttaa
     9901 tagtgattat tgtattttta caaaattttc gcgcttcatt agtacccgcg acaacggttc
     9961 ctgtgactat cattgggaca ttttttgcca tgcttttatt gggctatagt attaatttgc
    10021 tgactttatt tgctttggtg ttagcaattg gaattgtggt tgatgatgcc atcgttattg
    10081 ttgagggtgt gactcaacat attgaaaaag gcatgtcacc taaagaatca gccatacttg
    10141 caatgaaaga attatttggc ccgattattg gtatcacttt ggtattaatg gctgtttttg
    10201 taccggccgg ttttatgcct ggtttaacgg ggtcaatgta tgcacagttt gctttggtta
    10261 ttgctgcaac tgcctttata agcgcaatta atgccatgac gttaaagccg actcaatgtg
    10321 ctttatggtt aaaggctcct gatacgagta aaccaaagaa tatattcttt caggcctttg
    10381 atcgaattta taatcccatt gaatcggctt acgtaaagtt tattgatcgg cttgtacatc
    10441 gtagtggtaa agtttgtttg attggaatca ttttagtggc ttgtgccatt tatggcctga
    10501 cgcgtattcc aactggcttt attcccatgg aagatcaagg ctatttgatg ttaagtatcc
    10561 agctaccgga tggcgccagt ttaggccgta ctgatgaagt agtgagtaga ttagctaaaa
    10621 aagcatctga agttgggggt gtagataatg tgattgctat tgatggtatt tcattattgg
    10681 ataacaattc tctgctttct aatgccggtg tcatctatgt tatattcaaa gattggagtg
    10741 tcagaggaaa atctgaaaat ttacgagctc tttataccaa attcaatgca atagccaaag
    10801 aaacccttga tgcaaaagtg ttggtggttg tgccccctcc cattcaaggt ttagggatgt
    10861 cggggggatt tcaaatgcaa gttgaattac aagacggtac ttttgattac aggaaattac
    10921 agcaagctac tgatcaaatg atcaatacag ggcgtcaata cccacagttg caaaacctta
    10981 tgacgacatt tcgtgcgagt gtaccccaag ttgcagcacc tattaacaga accaaagccg
    11041 aatctttagg agtgcgtgtt gctgatgcct ttgatacttt acaaacttat ttaggctctt
    11101 cttatgtcaa tctgttcacg aaatttggcc aagtatttcc agtttatgtt caggcagatg
    11161 catcctcgag gattagcagc gaagatttaa ggaattacta tgtaagaaat caatccggct
    11221 ccatggtgcc cttaggtact cttacagacg tggggcctgc ggtaggaccc tcaattattt
    11281 ctttatataa cctttatcca tcaagcaaca taaatggggt tgctgcaaga ggttatagct
    11341 cggggcaggg aatccaggtg atggaggaat tggcaaagga acaattaccc ccagggatat
    11401 cttatgaatg gacaagtaca gcttaccagg aaaaggtggc tggtaacctc agttacttta
    11461 tttttgcctt gtctttggtt ttggtttatt taattttatc tggccagtat gaaaactggc
    11521 ttattccatc tgcgattatt ctgagtgttc ctttgacttt ggttggaaca gtattagctc
    11581 tcggcagttt gggtatggat aataatatgt acactcaaat tggcttgtta cttctcattg
    11641 cattggctac aaaaaatgct attttgattg ttgaggtagc gcgtgaacaa cgtgaaattc
    11701 ataataaatc agttcttgag gcagcggtca ttggcgccaa aacacgtttt cgtcctattc
    11761 tgatgacatc ttttgcattt atcatggggg ttatgccttt ggtttttgca acgggggcag
    11821 gtgccaattc tcgtcgctca attggtatag cggtgagcag tggtatgctt gcctcaactt
    11881 gccttgcagt tgtttttgtg cctgtttttt atgtattgtt gcaaacctgg caggacaaga
    11941 ggaaggcaaa acattaatta atttaacgta agttatggat aaagattaca taagccagtc
    12001 gatatctaga gtatgtttgg gctgaggaga tgcgtaagca tcgtctcgaa gccttggcat
    12061 gaaatattaa atcagatacg aggtttcgag ataacaactg attttatcca taactcacgc
    12121 taatttacag cgatagaaga acgctgtgag aaactctcac agccaaatag tgtatctatc
    12181 ttactgaaca ttcaaacagt tgatgctatc aagctatatt gtatgcccaa gggtgtctgt
    12241 acgaggctca tttcttccag tgatttcact cttacctatt tctttataac tcccgtgtac
    12301 aaagtgtctg taggaggctc atttcttcca gtgatttcac ccatcgcttc cctgaattgt
    12361 tttgatgttt gtttagtagt agggctttgg actgatttga ttggctcatt aagatttgat
    12421 tcctcaagct tttcagggtt agtagaccat tttataatgt tatttcttgt aaaatgctca
    12481 ggaggaattt tgtcagccag ttgactgagt ttttcatgaa cttgctcatc aatagctttt
    12541 tcttctttta gtttggagag taactgcgta ttgacttctt taaacagtct gggaatatct
    12601 tgctcaagga gagttttctt atcggttgat ttattaattt ccctgtattc caggcttaaa
    12661 tagctagcta cttttgctgc ctcttcaggg ttcaatgtct taaagaattc aattgcttca
    12721 tcattgtggc aatgagaagg caagtccctg acaatttgtt gaacagcagg atcttgtatc
    12781 ttttgaaatt gttcttccat ttccagagag atgatttcat caagttttgt tgatttaaag
    12841 gcatacttgt ccaattggca aatatctttc aattgatctc gaaataacct gacttccttt
    12901 tgcagagtac tttgaaacca attggattcg gcagaaatgt ctctgtcaat tttatctaat
    12961 gtttcaatag agtttaaaac cttttcaagg acctggcttt tcgttaaaga ttcactttgc
    13021 ataagagtat tgatttcttt ggatactctt tgtaattctt cacattgggt cttacgggct
    13081 tcatttcttg aacctttaaa ccaccattcg cgtcctctct tttcgttgta aatttttgca
    13141 gtgagttcta atccttcagc gattttattt aattccacat ccaataatgg cttggaggaa
    13201 tattttgacc aggtaattgc gcttagatta tcgtgttgta aattatcacg agaaagcaat
    13261 cttaaaagat cttgtttttg actctctggt atgctggtag ccttttccag ctctttttga
    13321 atggctggcc agttacgatt gtaagcaatg taattttgag tttccttcga aagcatggag
    13381 taatttggaa cacctgtagg tgaggtagca actaaataat ccaataattc ttttggttta
    13441 ttctcagcca gcagtttgac aacctctttc ttgaattgat ccagtttttc gtacccctgg
    13501 gccactacct cattcttgtg atgtaaccga ttggaggttc cttcctggag agcttcctga
    13561 tgacttgcaa gatcaccaag aggatgtttg cctttggttt gtcgggtaaa gaaatccaga
    13621 aaatgacaac tgattctgcc ttgatgagaa aagatattgc cttttttatc gggatcagcg
    13681 acaaaaaact catcgaaatg aggagattct ttaatcgtat tataatgtct tgtaaatttc
    13741 tcagtaattt cttgttgttg cttgcctgtt aacggggatt ttaatcctaa ctcggcatga
    13801 ttgctgttaa tatagtcgta tataattggt tctatctccg caccttgcgc aaatccttct
    13861 tttactcttt ttgcaatttc tgtagcatga ggctctttgt cgaaaaattc tccgaagtta
    13921 gcatccgata atttgtttgt tttacaataa atgtttgctt ccgccaacag gtactgaact
    13981 ctgatggata tcctgtctgc ttcgtggtct gattttattt ctttagcacc atcatagaaa
    14041 gggctggatg cttgagcttc ccatacagta ggatctattg atacttcaat aatcccttca
    14101 atccaatctt taatagtgga atcatcgtct aaagccatga cgatatccaa atagctcagg
    14161 ttgacttctt taccatcgcg actcatgttt actgacagat tggagtcaat ttttaccagc
    14221 tcttgttgaa tcagtttttt taactcttcc agtttttgtt ccttgtctcc atctttcata
    14281 tagtccgcat ctacttgtgc cagtacggtt tctactattt tttccttaga tgatttttta
    14341 ttgattggag tcggtgtttt atcttccggg aagagagttg aacgcaggcg ataccctaat
    14401 ccctcgtata ttggaatagg agcttgcctt gagggcgtat cgtatttaga gatgtttctt
    14461 ttgacattga ataaagggtc atcaaagcga gtaaatttat cgttgtcata aggagaaagt
    14521 ctcacagcga aagcattttc agaggattta atgatttcct ttacaccaga gggtaattgt
    14581 ggaatccctc cagtacggag ttgcctgatg tcattttgct catcatattg tttttttagt
    14641 acttgaatta attcaatgta cttttcaatc tgttgcaggc gttccgtttt ctcttttaat
    14701 aaatcgtcat aagcatgagg tgaaattttc ttttggctat taatggcttt gatgtcttct
    14761 tccaattgcc ttttgtattc actaagttgg tgttcggcag aatatttggt cacaccactg
    14821 tgtgcactgc catagaaaaa cgtgatcaat tcaactgctg tttgacaggt gttgtctaaa
    14881 cctatttcat ctcctcctac aatcggtaaa aggatatgaa ctttgttgtt ttcatcaata
    14941 tataggtagt cacaacgttc cggctctttg aatttgatta cgtcaaccat gtttatcacc
    15001 attttttagt gtcttgttta taagtatatt ataaagacaa tttgtttata aaacaacaaa
    15061 aagagttgca attacacttt ttgtttgatt aaacataccc cgcaatctaa ctgtattttt
    15121 tattctcaat tgcttgattt gatattatca agttatatcg tctgtctaac cgtttctctg
    15181 ggcggctcat ttcttcctgc catttcattt atggtttctc tatattttct tgccatttca
    15241 gaaagtgagc catcagatga ttttagtaat tcgccgagat tggactcctc aagcttttca
    15301 ggattggcag accattttct gatattattc cttgtaaaat gttcgggagg tattttgtca
    15361 gctagttgag ccagtttttc atagacacct tctcccagaa cactatcttc ttttaatttg
    15421 gataatagct gcatgttgac ttctttaaac aaattgggga tatcctcgtt caagagctta
    15481 tccttatctg tggatttatt aatctcccgg tattccaggc ttaaataact ggctacttta
    15541 gctgcctctt cggggtttaa tgtcttgaag aactcaattg cttcgtcatt atggcagtgt
    15601 gagggtaagt ctctgacaat ttgttgcaca gtagggtctt taatcttttg aaattgctct
    15661 tccatttcaa gggagatgat ttcaccaagc tttgttgatt taaaggcata attgtctaac
    15721 tgacagatgt ctttcaattg ttctctgaat aatctgacct ctttttgcaa agtactttgg
    15781 aacaaattga actcggagga tatttcatca tcaattttat ccagcgtttc tatcgatttt
    15841 aatagtgtat taagtacttc accttttgat aaagatgggt tgtccaggag ggtattaatt
    15901 tcttcagcta ctttttttaa ttcagcgcat tgagcttccc gtgcttcatt tcttgagcct
    15961 ttgtaccatt gttgttgcct tttttcgtca taaatatctg ctgttaaatt taatccttca
    16021 gcaactttac ttaattccac ttccaaaagt ggctttgaag agtattttga ccaggtaatt
    16081 gcactaagat tgtcgtgttg tacgttatcg cgagaaagca atcttaaaag atcctgtttg
    16141 atattgggtt gtatattgtc agacttctgt aactcccgtt caatggcagg ccagttgcgg
    16201 ttataagaga tgtaattttg ggtttccagt gaaagcaagg agtaatttgg cacgccagtt
    16261 ggtgatgtcg ctgttaaata atccagcaat tctttgggtt tattttcagc cagaagcctt
    16321 actacttctt gtttaaattg ctctattttc tcatatcctt ccgccacaat ttcattttta
    16381 tgatttaagc gatttgaagt tccttccagt aatgcttctg cgtgaccgtc aagttctccc
    16441 aaaagatgtt tggcgttagt ttgtcgggca aagaagtcaa ggaaatggca actgagtctg
    16501 ccctgatggg taaatatatt tcccttctta tcaggatcag cgataaaaaa ttcatcaaaa
    16561 tgaggggagt ccttaatggt attgtaatgt tgcgaaaatt tatcggtgat ttcttgttgt
    16621 tgtttggcag tcaaagggga ttttaatcca agctctgcat ggttgctgtt gatgtaattg
    16681 tatatgatag gctctatatc tactccttgc accaatcctt ctttgactct tttcgcaatt
    16741 tcagtggcat gaggctcctt gtcaaaaaat tccccgaagt tagcatcgga taatttgttc
    16801 gttttacaat aaaaattagc ctcggctaat aggtattgta ctctgatgga cattttatcc
    16861 gcatcgtctt tttgttttat ttcttttgcg ccatcataga agggactggc ggattgagta
    16921 acccaaacgc tcgaatctac tgtcgctgtg agaatagaat caacccattc ctggattgaa
    16981 ctatcctcat ccatgcccat catattttcc agatagtcgt agtttgtctc ttgtttatca
    17041 tggcttatat ccactgacaa attggagtca atttttacaa gctcttcttg aagaagggct
    17101 tttagctctt tcaatttttg gtcacggtct ggttcagcta ttttttctgg ttgaaattta
    17161 gctaaaacag tttctacaat tttttctttt tgtgattttc tattaattgg cgtaggagtt
    17221 tcaggatctg gcaagagcgt tgaactgagg cgaaccccta agccttctgt cagtttataa
    17281 tcgctggttt cataatgtct atttaacctg aataaagggt ttttaaaact ggttgccaaa
    17341 tcagggcgat ctggggacag tcttacagca ccagcattct cagaagactg gattatttgg
    17401 tttaatccgg aaggtagagg tggaataatg ttatttttta tagtcattat ttcaccgttg
    17461 tgatcatatt ctgttttgag tacgttaatt aactcaatgt atttttcaat ttgtttaagt
    17521 cgttcctttt tttcccttaa cagatcggta taagcataac gtgatatccc tttttggcta
    17581 ttgatggctt ttatgtcttc ttccagttgt tttttataat cagtcagctg ttgctcagca
    17641 gaatgcctag cttcgccatg gtgagtattt ccgtagaaaa atgatcttaa ttcaactgct
    17701 gtttgacaag tgttatccag tcctatctca tcgcctccta caataggcat gagtaaatgg
    17761 actttgttat tctcatcaat atatagatag tcgcatcgtt ctggttcttt aaatttgatt
    17821 acatcagcca ttttgatcac catatttcag tgcctcaatt ataagtatat tataaaatca
    17881 ttatgaattt taaacaatca gttagattgt taacaaatcg ataagattta tgttttttct
    17941 attagtgtaa aaaaccaatc attgttcttc tatacaataa tcgagtgcac aatctaagtt
    18001 atctaaaata atcgaatatg agattttcga ttaaatcatt gcgatttttt gctttatacc
    18061 tatcgcaatg aattgataaa taattacact tgttatataa taaatcctca ttttgtttat
    18121 ggaaggagac cattgtgcct attattctag attctgatgt gttggaagtg gcggaatatg
    18181 tgtataaaac acgcttatct cagccttaca ctgaagtggg ttctgaatgg gagtataatt
    18241 acaagaatcc aacagccact tttgcaaaag gcgatggcca taacttgcaa aggtatatta
    18301 caattgatgg caagcagctt catcgcccta tccatggctt agctcatacg atgcgcacat
    18361 tgatgtatag ccaattgatg tattgctcct caaaaaaaca actttcccca cacgtatgtc
    18421 aagacggacg aacaatagcc gatctcagtg aacttgatct taaaaagata aacattgcgc
    18481 agttattttt tgtagccggg cgcgaaagcg aggcctctta tggtgatgct tatcatcgtt
    18541 atcatctgta tggggctaaa cagttcgaag aatatgctcg caagcatttg actcatcttt
    18601 tttcagaaga agaaatcata ttatactcac ggtgtataga agacagagta ggtgatagtt
    18661 ttgatggtac ccctgaaggt tatataattc acttatcaca catgatcgat ttgatgcgtt
    18721 gcaaaagccc ggttgaagtt tttttgggac actcaaaagg ggtttctggt attgtaccta
    18781 cattgattca cttgtttggc aaacaagatg gcttggatat tatgcattac gcccgaggct
    18841 tgtttgcggc tacaggtgaa gcagtaccct atattgattc atctgaatgg cctcatttgg
    18901 gagttgattt gagtcgggta caacgtgctc tctctatcgt tggtgatatc aatgtccctg
    18961 ggcaagaggc ggattctaaa aaaacagcgc aagccggttt ttcagtggat ggttgttatt
    19021 ccgcgttaac cagtgtgcca acaccttcct ggtatgagaa agagttaaaa gaagtcgatg
    19081 atgaaaaaat cgatgttaag gaagttgatg atagggagat agaaaaagaa catgagatag
    19141 ttgttccttc gcaggcaaca acacctttaa gtacagaaaa acccggctct tttgtcgaaa
    19201 aatttctgaa gccatttatg atttggaaaa agccagaggt acaaacgact cagccaacaa
    19261 cggagaagac taataaacct taacctgttt tgcttaaagt agttagagaa acgaattctc
    19321 ctagggaatt aaaacgcaat gaggtgttgt aactatgttt agttatctgg ataaattatt
    19381 agatggaata tttggtaacc aaacgcctga taccagtcag ttaagcacct caaagtcacc
    19441 tactttaact actgttccct tgaaacaaga atattttgta aaaatcgggg aaagtgaaac
    19501 tcaggatctt ggcttactgc ccgtggttag caagcgacac aatcaatcag tgcctctgga
    19561 ggaaatccct ttcgaggaaa ccaggcttga gttgattgga ctatatatta cattgttaaa
    19621 acaatgtgtt ttggatgaaa aactgaagag catccctgcg cagtatctta tatctcatta
    19681 tctttttata aaaacattag ccgcaaatga gggaaataaa ggtcgaaaag atctgtatct
    19741 taatttagct caaaaagtgg ctgattatct tgaaaaaaat gaatctaaaa tatggagtat
    19801 ggctgttgaa tgcactaaaa ccagtgagta tcccatagtt gactggataa aaaaacacca
    19861 tcttcatttt aattttataa gagcttttat actggattac aataaaaaac cattgactca
    19921 taatcaacgt gcgtttatgc agcagtttag ggattccgct gcttttttct ttcctgatca
    19981 ggtttacctt gcttggctta cccaatccta tgaatcaggc tctatcttga atcctatgta
    20041 cagggagagc agatcaacgc attattatca cgcgaacatt actgataatc tcttgttaag
    20101 aacacgcccc aaacaggtca atcttggtcc tcaacatttt ttccaaaaag gaaaaggctc
    20161 cgtcaagaat acttatcgtt tcaatatcaa tgacggaaaa ttaatgcgta tacagggaag
    20221 aacattactt tttagcacga ataaaggtaa tgaagtgatt gctgtaaagg tgcaaaaaaa
    20281 aggggaacca caatctgctt taagtaatga atttcaaatg gcagattatt tattgaaaca
    20341 tcaacgtcgt ttgaatttgc aaagtcaatt acctacccct ctcagccaat attcgattaa
    20401 tcgaacagag attatcgaaa aatgcagtaa atcacctgat tttgagaagt ttaagaattt
    20461 gataagtgat gcaaaaagcc ttgaaattta tgtttataaa gcaacacctt cttattttac
    20521 ttatctgcat gataagcagc agagctttag tcaactcact tcatcagtgc agaaaaatgt
    20581 acatgatctt ttcatgctcc tgagagaggg catagtcttt ccttatctgg cagatatgtt
    20641 tcacacccat attgatgaga gtaaaaggag cgataagggt cgatatcaga cgttagttga
    20701 gcttttaagt gctttgcaat cacaaatggg ccgtcttgat aaatggcaaa aagcagtcga
    20761 atttgttaac ttacgagcca gcggtatcgc tgatttgggt gacaatcttc cgttgattag
    20821 cttcttaact gtttctgact ggacgaaaca ttatcatgcg gaattgctaa cgggcgttta
    20881 ccatccatcc tttctattcc tcgataaatc cagtggctca gttcgctcct tgtttaacag
    20941 ccggcgcaaa gtattcggca attatttgta tctgaatatc atcgctgagt atctactagt
    21001 tattcaattg gtaattggct gttatggcga taaagtgacc cgtaaaatgg atagtaaaag
    21061 caaagctaag gtttgggagc atttagctga attgatgttt tccagttgcg ctgaagcggt
    21121 taatttgata actggtatgc ccaaatccag agcacttgct tttttaaagc aaagagccaa
    21181 tgtaagaaaa catactcaac aaacgtcgtt ttggatgact cctgattatt caaatctgga
    21241 taggctgagt gtgcagtctc aacaatccac tttgtacccc ggcgaatcag attatgaaat
    21301 aaacagtgat ttaattccgg gggtaggcct gagtcttgat ggcatcaatc aggatttagg
    21361 tgattataac caggcaagcc ctttaaggga gctagagaaa ttattgtatg ctacagtgac
    21421 cttaatagag ggaacccagc aactggataa gcaatttttt caacagttgg atgaaactga
    21481 gaaaatgatt ttgagtgctg ctggagttga taaatgctat caagctgtcg caaaactcct
    21541 agatcttgct cggcctggtt gtcaaatgca gcgaagattg gcttttactt attatgaaat
    21601 gatcaaacga atatatccct gctcaaatcc tgcagatgtg agatttgaac ttgttgcaaa
    21661 agaagaggcg attataaaaa ttcagcgttt ttggcgagag cataaaaaag aaaaccaatc
    21721 gattgaaaaa gggtttgatt ttgacaggaa cactagctca tcgcagtcgc ctttatgaaa
    21781 aagtcatttc aattgtaaga gatcatcccc gaccgttaga ggatatttca acatgagggc
    21841 actgacctgt tatttttaat tttggagaat taaaagtact cataagctta atgagatttt
    21901 tcttatttcc gctaacaatt ggattttatg ctatttaatt aagacaagat tttattattc
    21961 atccagggaa ttatggtgga agtttaaact caatatcaga tatgattacg ctttgaattg
    22021 acaaaaacaa gacaaaaaac gatacttttg cttttataaa gaaagcaaat ttttttaaaa
    22081 cggtcaataa tatcttgttg attaaataat gttcaaatgg tataatgttt aattaacttg
    22141 ctggacttaa cttatttcat atgaagtgtg aatcatgcct gaagagacga gcacaaattt
    22201 accaaaatca ctcagccatt ttcttattca atctgatttg gattataaga actcattgag
    22261 ttcgattcct ttatttgacg ttaagcgaac tattttctgg gatgacaacc ttagaaaaat
    22321 gtttgcatca attttttatc atttaagagg gcattttata aattttttgt ggtacgttgc
    22381 caactttgct gataacgaag cttcaaaaca aattattatt caaaatattt atgaagaaat
    22441 tggaattggc aatcgctttt cccatgaaaa gctttatgaa cgttttgcaa aagaatgcgg
    22501 tgttgatatt catgatgaaa tagtgaatga aaccaactat attccttttg ccaaagaatt
    22561 taataaagct catttatctt ggttggcgca acatgatcct atagatcatt tgtgtgcttt
    22621 tgcggcttat gagcgcttgg ataatcttga ttatcctttt cttgttgaac ttgccaaatc
    22681 gatgaaaata tctcaacatg gcatgacctt ctttaatgta catacccacg ttgatcattt
    22741 tgataccacg ctacaattga ttgtgcccat atgggaagaa tcacctcagc gtctgatcga
    22801 atcatttcat tttattcaaa ctcatcagtt agggatgtgg gaacagtttt ccaatgtctt
    22861 atttaattga tattttttaa cataagaaat cttgggattt cttatgttaa ataggcacta
    22921 atcaattttg gggaatttta aagtgaaatg agcgaattca ttttttttcc ctttacagga
    22981 aatatcacca cctgctgctt gcatcagaag tttacaatag gccaagccta aaccagttcc
    23041 ttcttttctg ccagaataga agaaatcaaa tattttttca tattctt
//
LOCUS       DAXLDT010000043        22490 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXLDT010000043 DAXLDT010000000
VERSION     DAXLDT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22490
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.043"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..677
                     /locus_tag="ACF2SP_001291"
     CDS             <1..677
                     /locus_tag="ACF2SP_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFD2359084.1"
                     /translation="GTVSHGLLSYLQIMYHSVFGLNSKHELQALNLLHELMAKIYRNI
                     FNELGDSSFAIIDLASSFDYRNGKLYQAQIEPSDEGGELIASLIAHTITHHDFQNESM
                     LYAKPPLQLNPKILAVPLTKLKKDWFPRQIYVTQEQAISAFKQAYLLQLNKDRQSYLG
                     LYGFFARSNVDFEHATLEKLIEHAQNYSTKKTGHRTLKVMQNLGWLTEQGTIKEESGI
                     GRLLKN"
     gene            complement(790..1974)
                     /locus_tag="ACF2SP_001292"
     CDS             complement(790..1974)
                     /locus_tag="ACF2SP_001292"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFD2359085.1"
                     /translation="MTDVYVVDVLRTPVGKAPRGVFKNTLPDDLLAHTIKCLIHRHQN
                     IDWQEIADVVIGCAMPEAEQGMNVARIASLLADLPQSVPAMTINRFCSSGIQSIVTAA
                     DTIRNQEAHLALGGGVESMSMVPMGGNKYTANPSIFNDEHVAIAYGMGITAENVAKRW
                     EITREQQDEFAIESHKKAIAAQERLDFNAEISPIEISIRYPDLKTSKVEIKKKLINKD
                     EGPRADTSYEAISKLKPVFALNGTVTAGNSSQTSDGAGVALLASEKALKQYDLKPLGR
                     LMSYAVAGVPPEIMGIGPVKAIPMALKRAGITLDQLDWIELNEAFAAQALAVIKDLDL
                     PREKVNPLGGAIALGHPLGATGTIRTATLLHGLRRIKGRYGMVTMCIGTGMGAAAIFE
                     AL"
     gene            complement(1990..4359)
                     /locus_tag="ACF2SP_001293"
     CDS             complement(1990..4359)
                     /locus_tag="ACF2SP_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HFD2359086.1"
                     /translation="MQEPFFIKKVAVLGAGVMGAQIAAHCVNAGIETLLFDLAGKEGP
                     KNSLVDKAIANLGKLKPTPLATPQTNELLQAKNYAENLEELSSCDLVIEAIAERIDWK
                     EDLYKRISPYLSDKTILVSNTSGLSINKLCEVLPAQHRSNFCGVHFFNPPRYMHLAEL
                     IPANTTSSDLLDYLETWLTEYLGKGVVRAKDTPNFIANRIGVFSLLTTLHHTLAMNMG
                     IDEVDALTGPLLGRPKSATFRTMDVVGLDTMQHVINTMQSQLTGDPWHKMFILPDWLN
                     GLIKEGHLGQKSGQGIYRKAGKVIEVYDIQTGQYRPSRAEASDELKAIMDIKDPEARM
                     KNLMTSNNKQAQFLAACFRDLFHYCAYHLEEIADNARDVDLAIRWGFGWAQGPFETWQ
                     LSDLHSVTRQIDESIQDNTALSSAKLPEWLYEIKEFYTREGAYSPGKNNYHARSQLPV
                     YHKQFFHDRVLKESHHPVHVIYENEGVCLWYLKDDVAVISFKSKANTIGQSVLDGIET
                     ALDIAERDYQGLIIYQQDASNFSSGADLRGVFKLLTDGTIQALEHMVEQFQRVAMRLK
                     YSSIPTVAALRGRALGGGCELMMHCDSIVAAFESYPGLVEAGVGVIPAGGGCKELAMR
                     SAHHAQQADLLTFILPVFQQIATAKVAASAVEARNMGYLRAADSILMHADEVLYASLE
                     KIKAMRAANYLPPMKKQRFKVAGIEGHARLQSGLVNWLEGGFISQHDYFLANELAKVL
                     CGGDVNHGTLVDEQWMLKLEREAFIKLANTSLTQARISHLLETGKPLRN"
     gene            complement(4479..5999)
                     /locus_tag="ACF2SP_001294"
     CDS             complement(4479..5999)
                     /locus_tag="ACF2SP_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFD2359087.1"
                     /translation="MDLLQRLNIKSVNPGAFSGHGWHSDNHAHTLESFNPSTGNKLAE
                     IATCTMDDYEQVMQRAEHAAQAWRKVPAPKRGEIIRQIGQALRENKDSLGSLVSLEMG
                     KSKQEGDGEVQEMIDIADFAVGQSRMLYGNSMHSERPNHRMYEQWHPYGIVGVISAFN
                     FPVAVWSWNAFLSAICGNVTIWKPSAKTPLCAVAVQHICNQVLKENNCPEIFGLIIPN
                     SHDVVEAMVDDKRIQLISFTGSTAVGKQVAAKVAARLGKSILELGGNNGIILDESADL
                     NLAIPAIVFGAVGTAGQRCTTTRRLFVHESKYQDVIKRLRHAYEQITIGDPLDTRNLM
                     GPLIDQQAVEQFKKAINRIKAAGGQIVYGGEILKQAGSFVQPTLVCDVKNDWDIVQEE
                     TFAPILYVMSYRTLDEAIALHNGVPQGLSSALFTQNLKNAELFLSACGSDCGIANINI
                     GTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINWGDELPLAQGIRFNLS"
     gene            complement(6009..7097)
                     /locus_tag="ACF2SP_001295"
     CDS             complement(6009..7097)
                     /locus_tag="ACF2SP_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HFD2359088.1"
                     /translation="MYNVMITGAGKIGSLIACLLADSGSYQVHLADLEFNGSDVTRLL
                     TALPEIKTVALDVKDEQSTQAYLQKHNIIAVISSLPYFLNTHVAKAAKAAKAHYFDLT
                     EDTSVTEAVKAIAQNAETAFVPQCGLAPGFISIAANSLMQEFEKCHHARLRVGALPQR
                     ANNALHYSLTWSTDGVINEYGNPCYGIEGGKDVVMAPLEGLESIQIDGCEYEAFNTSG
                     GLGSLAELYAGKIQSMNYKTMRYPGHCKKMRFLMNDLRLNEDRGTLKRILENAIPKTY
                     QDIVIVYVTVEGIKQGELTEKSYVKKIYPEVIRGLEWSAIQVSTASGVCAVVDLVLGQ
                     GNEYKGLILQEKFKLSHVLENRFGKYYA"
     gene            7251..8786
                     /gene="lnt"
                     /locus_tag="ACF2SP_001296"
     CDS             7251..8786
                     /gene="lnt"
                     /locus_tag="ACF2SP_001296"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFD2359089.1"
                     /translation="MKQAALIHSTIINTSTLNQYSKFIVVFLAGLLAPLGFAPFHLPG
                     LTILSLAILFSKFLQCTVKQGFTLGFLYGLGYFGFGVSWVIISIHDYGQLDYFLAGVI
                     TLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLT
                     GITQIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFTLILIAPS
                     LGKNIHWTEIKKEPISVGAIQPNLSMRDKWDETLFWDLLKYYENKTDKLLGKNLIVLP
                     ESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNSIISLGKATGAY
                     VKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYP
                     NILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVI
                     NHQGTIVEGLPAFNSGMLQTDIFPANGITPWIIWSDYPAFIFCFIFVLFVLFFNIKRF
                     TEK"
     gene            8852..11323
                     /gene="leuS"
                     /locus_tag="ACF2SP_001297"
     CDS             8852..11323
                     /gene="leuS"
                     /locus_tag="ACF2SP_001297"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFD2359090.1"
                     /translation="MDNTYNPQEVEEQAQQYWHKKQSFNVTEDLNKEKFYCLSMFPYP
                     SGTLHMGHVRNYTLGDVIARYQRALGKNVLQPIGWDAFGLPAENAAIKNKIPPAEWTR
                     KNIAAMKEQFLRLGNAYDWKREITTCDPEYYRWEQWFFIRLFEKGLVYKKNAVVNWDP
                     VDQTVLANEQVVDGRGWRSGALVERKEISQWFIKITSYADELLSSLDSLDEWPAQVKQ
                     MQRNWIGKSIGTEIYFNVNNYPKRLKIYTTRPDTLMGATYLAVATDHPLAKEAASNNK
                     KVQEFLDSCQGIKIAEAELATMEKRGIDTGMTAIHPITGKELPIWVANFVLMQYGSGA
                     VMAVPAHDQRDWEFAQKYQLPVKQVIKPIDIEHDFNQSAYTEEGILINSNQFDNLLSS
                     KAIQVITNFLEENDAGKATINYRLRDWGVSRQRYWGTPIPMIICEQCGIVPVPDEELP
                     VVLPENVDFTGTGSPLTQCKEFVNVTCPKCGQDANRETDTFDTFVESSWYYARFACKG
                     QENAMLDDRAKYWTPVDQYIGGIEHAVMHLLYARFFHKLMRDEGLVNSDEPFKALLTQ
                     GMVLKDGHKMSKSLGNVVDPNHLINTYGADTARLFVMFASPPEQSLEWSDSGVEGAHR
                     FLKRVWAFSHQHRDMLIDINDSILSGNGHVDWKEAESRLKKSRHIVHQILAQATHDYD
                     RNQFNTVVSGCMKLFNEISDYSIETENDKFFIHSSISILLRLLAPITPHICHCLWQQL
                     GFDKAIIDAPWPKVDKSALKTDEVDYVVQVNGKLRAQFTASTDASEEELIAAAKEHAH
                     NFVVNHTIKKAIIVPHRQLINLVIG"
     gene            11424..11915
                     /gene="lptE"
                     /locus_tag="ACF2SP_001298"
     CDS             11424..11915
                     /gene="lptE"
                     /locus_tag="ACF2SP_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFD2359091.1"
                     /translation="MTQIHKYLASLILIWLLTGCGFHLRGAIDIPEWLDNVSIISRDG
                     NKELVSILKSQLEGYKIDVNSDPSLAKYWLIINSSSLQQQIVSIGASTNPRQYQLILT
                     IEFMLQTPKGQIIKTPRKVNVSRQLTVNNDRILGSNEEQALLVSEMRRDAAIQIINRL
                     NRP"
     gene            11917..12942
                     /gene="holA"
                     /locus_tag="ACF2SP_001299"
     CDS             11917..12942
                     /gene="holA"
                     /locus_tag="ACF2SP_001299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFD2359092.1"
                     /translation="MQINQQALTQLLQKKIHHLNVLIGQDHYLIDEALNNIKSMLKKS
                     SECDEKILSIQSPEEWHVVLEETNNYSLFYQTVILTIFFDKKTIDTMGKKILSEYLKS
                     TNSHSYIIIRAPNLTAKQLQWLTHDEQALIIIAQPLSSEAMKRWINEQLKLNTISFDP
                     YVPELIYQYTKGNMLATAQVIEKITLSNTPGTLITPYLALEHLSDQCDFSLFELIDSC
                     LLGHSDRAIHILRQAANNKTEVTLVLWMLTQEIRTLLQLLFLMQQKMDIKMASNQLKI
                     WTQRVNLYQSAVKRLNTLLLQQLHHYCQSIDDKIKSNSSNQAWNALENISLSLCLGQL
                     LGDACIA"
     gene            12930..13565
                     /gene="nadD"
                     /locus_tag="ACF2SP_001300"
     CDS             12930..13565
                     /gene="nadD"
                     /locus_tag="ACF2SP_001300"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFD2359093.1"
                     /translation="MHSIAIFGGTFDPVHNGHIKTSLAIQANFGFDSYYFLPCKSPAI
                     KPPSFASSEQRVEMLKLALKPYPDFKIDTRELDRDTPSYMVYTLQSFRQEYTDSSLTL
                     IIGYDGLLNLPQWYQWEKIISLANLLVINREEFFQQPVPKSVQTLLNQYRNDDKNILL
                     NHHAGSICLYNAGHYDISSTKIREQLKQHKDVKSNLPDLVYDYIKKQELYQ"
     gene            complement(13540..14073)
                     /locus_tag="ACF2SP_001301"
     CDS             complement(13540..14073)
                     /locus_tag="ACF2SP_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFD2359094.1"
                     /translation="MQLQWVDIAIVAIIALSVLTGLFRGFVKELVALCVWILAIWLGF
                     NYSQSLDPWLSSYIEEQSVRTAIGFIIILFATLFVGGVVNAILSFILKRAGLSGTDRT
                     LGMGFGFLRGVFIVALIMVAVKMTSLPYQEYSQKSKLYARFDPVVALLYSHLPEFIKQ
                     VKTVDKTENIIDTVPAS"
     gene            complement(14054..14842)
                     /locus_tag="ACF2SP_001302"
     CDS             complement(14054..14842)
                     /locus_tag="ACF2SP_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFD2359095.1"
                     /translation="MKLIMDEKVKHRLIGLAVIISLGAIFAPALIKKSSQSPESNFSV
                     NVKLPPKPLPPDVVITDEKEVFKTIKVAKTEIPPIPVEKQSVSLVKAEPISTEEDNLN
                     RTSKIATLEADRENSIKSEPIKLAVNKAANATAKKVMTLAAAKPPAKRSPVVTAKVNT
                     NKKVSQIVAARKPAVKQDIYAVQLASFTQIANAQALVNRLRSKGYKANYLKSNSRNGV
                     VYKVVVGHSPVKKDVIKLKTQLASAMQLNGFVVNTGVSYATTMG"
     gene            complement(14839..16125)
                     /gene="folC"
                     /locus_tag="ACF2SP_001303"
     CDS             complement(14839..16125)
                     /gene="folC"
                     /locus_tag="ACF2SP_001303"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFD2359096.1"
                     /translation="MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRP
                     NCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTSPHLLVFNERIRINLAPIQDKDL
                     CKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
                     AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSLPKSVVEVAVQLGALSY
                     LYGKDYFYQEEADHWGVTCQGKPFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYENL
                     KKAMDLTDIPGRLQLQKGKTDILYDVSHNPQSVRLLADTVKKINVKGQIHAVFSALKD
                     KDLYGLIMPLKDCVNRWYPAQLHNKRAASQEELLSVFREAEIFADVCYNDPFAAFEKA
                     LKQASDDDLIIVYGSFFTVSHVMAAQHNLLEQKENQ"
     gene            complement(16106..16990)
                     /gene="accD"
                     /locus_tag="ACF2SP_001304"
     CDS             complement(16106..16990)
                     /gene="accD"
                     /locus_tag="ACF2SP_001304"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFD2359097.1"
                     /translation="MSWLKKLLPSRIRTETSQKKGVPEGLWVKCSGCNEVLYSTELAK
                     NFMVCPSCNFHHRISARLRIAQFLDESGQEEVAAGLEPQDRLKFRDSKKYKDRISQAQ
                     KATGEKEALIVVKGFLMQQPVVVSAFEFNFMGGSMGATVGEKFVRAVNVAATEKRPYI
                     CFTASGGARMQEGLFSLMQMAKTSAALAKFAETGLPFIVVLTDPTMGGVSASFGSLGD
                     IIIAEPNALIGFAGPRVIEQTVRQTLPEGFQRSEFLLEHGHIDMIVERKHLRATISEL
                     VSKLTHKGAESFFAHAME"
     gene            17400..18827
                     /locus_tag="ACF2SP_001305"
     CDS             17400..18827
                     /locus_tag="ACF2SP_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFD2359098.1"
                     /translation="MAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDD
                     AAGLAISQRMTAQIRGMNQAVRNANDGISLAQVAEGAMQETTNILQRMRELSVQAANS
                     TNNSSDRASIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASFQVGANSNQTINFS
                     IGSIKASSIGGIATATGTEVAGAAATDITIAIGGGAATSINSSANFTGALNGQDATSA
                     YAKAAAINDAGIGGLSVTASTSGTQAVGAIGGTAGDTYNLTINGVAIYTNLNVATALT
                     NSDLRDAINGVSNQTGVVASLNGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGA
                     FDGALRGTLSISAVDTIAIGGTVANVGLSANIAKDTVGVDSLDVSTAAGAQTAIKRID
                     AALNSVNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTKNQI
                     LQQAGTAMLAQANSLPQSVLSLLGR"
     gene            18910..19191
                     /locus_tag="ACF2SP_001306"
     CDS             18910..19191
                     /locus_tag="ACF2SP_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359099.1"
                     /translation="MSIEPVPSVNRAAQTDQTKIVEEVSKPEIKNMVYDSALDAETKQ
                     ALDKATGLLQTIITEKISDKVLRKMPSDEYLQLLSLLDDIISGSIDKHV"
     gene            19254..20879
                     /gene="fliD"
                     /locus_tag="ACF2SP_001307"
     CDS             19254..20879
                     /gene="fliD"
                     /locus_tag="ACF2SP_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFD2359100.1"
                     /translation="MELSSPGVGSGLDVKNLVDAIVTAEITPSQVRHDKQLNSVNTEL
                     SALGQLKSYLTNLQTSLAKLSSLSQFYTMKSTISDPNYFSATLGTGATKNTYQIEIQK
                     LAQQHSLASSYMTNTGSGTITIDFGTYNSDKTTFTPNPSATSVNITIAPGNDSLVAVR
                     DAINNTNSGITASIVQDSQGSRLTITSSKTGENYAMKISGGVASLNYDPTMGVNSLTE
                     TVAAQNSTVKINGLTLVQSSNQLNEAITGVTIDLKKAEIGKIITLTVDDNKDQLTGLI
                     NEFIKQYNDTMTFLTNLTGYNNETKQGGVFQGDPQFRNLKLNLNKWATTPLANQEGPI
                     RSLADLGIITNKQGLLEIKQNKFKTALDNHYKEIGALFAKTAKTNDTGVNIKSINSTV
                     KSGTYDVVLTEFTPGVSMSGTIGGLSATSSDGITLTGSGDLSGLSINVFSGSAGSRGQ
                     ITVTDGIAIQLSSLLDAYIETKGDLEQRKDQLNNQVKQLNKIQNEIDIRRKSIETRYL
                     KQFTALDTLLSQLQSTSVFLSQQLANLPQLKLK"
     gene            20889..21299
                     /gene="fliS"
                     /locus_tag="ACF2SP_001308"
     CDS             20889..21299
                     /gene="fliS"
                     /locus_tag="ACF2SP_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFD2359101.1"
                     /translation="MRMKNLYSQISSQYKAIELQTRIETASPHELIDLLLQGARSHIA
                     TAQGNIQRNQIKEKGEHISKAISIIEGLKMSLNHDKGGEIAENLLQLYDYVQQILLKA
                     NLKNDEDLLAQSNMLLAEVHQAWQAINSNAGNEL"
     gene            21622..22368
                     /locus_tag="ACF2SP_001309"
     CDS             21622..22368
                     /locus_tag="ACF2SP_001309"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFD2359102.1"
                     /translation="MNTFKSSLASVICLLLSMQSAQAQMRYGETLCNLPEYFCMKIKS
                     GDSWDRLFPNIEEQDIVRRINRMNIRLKPGMIIAVPKNIERLTIYDVSPFPRYIEPSG
                     EKTIYVSQKKLAWGAYDEDGELLWWGPISSGVGKCKVIGGCSTPTGSFRIIRKQDIDC
                     VSTVFPRRANGEHGGALMPYCMHFFRGYALHGSYEVPGYRASHGCVRMFIEDARWLNE
                     EFIDLPGGGMRGTRVILDAPDAQENPDNLQ"
BASE COUNT         7041 a         4657 c         4051 g         6741 t
ORIGIN      
        1 acggaactgt ttcgcatggt ttattgtcct atttacaaat aatgtaccat tctgtatttg
       61 gtttaaattc taaacatgaa ctacaggcgc taaacctgct tcatgaacta atggcaaaaa
      121 tatatcgtaa tattttcaat gaacttggtg atagttcttt tgccattatt gatctcgcta
      181 gtagttttga ctatcgaaat ggcaaattat atcaagctca aatcgaacct agcgatgaag
      241 gaggtgaact gattgcctct ttaattgcac atacgattac acatcacgac tttcaaaatg
      301 aatccatgct ctatgccaaa ccacctttac agcttaatcc gaaaatactg gcagtccctt
      361 taaccaaact taaaaaagat tggtttcctc gacaaatcta tgtaactcaa gaacaagcaa
      421 taagtgcttt taaacaagct tatttgttac agctaaacaa agatagacaa tcttatttag
      481 gtctatatgg tttttttgct cgcagtaatg tagattttga acacgcaact ttagaaaaac
      541 tcatagaaca tgcgcaaaac tattcaacta aaaaaacagg gcatcgcaca ctaaaagtta
      601 tgcaaaatct aggttggctg acagaacaag gaactataaa agaagaaagt gggataggaa
      661 gactattaaa aaattgaatt atgtgtagta gttcaaatct gctgatacta atcagttctt
      721 ggcaccaagc tttgcttggt gccaagaacg tcttatacta gcattataga ttggattaaa
      781 atgatatatt tatagcgctt caaatatcgc agcagctccc attccggttc caatacacat
      841 ggttaccata ccgtagcgcc cttttattct tctcaatcca tgtagtaatg tagctgttct
      901 tatggtgccc gttgctccta aaggatgtcc caaagcaata gcccctccta aagggttaac
      961 tttctctcta ggtaagtcaa ggtctttaat aactgccaaa gcttgggcag caaaagcttc
     1021 attcaattca atccaatcca attgatctaa agtaattcca gcacgcttta gcgccatagg
     1081 aattgccttt actggtccga ttcccatgat ttcaggcggt actcccgcaa cggcatagct
     1141 cattaaacgg cctaaaggtt ttaaatcata ctgttttaaa gccttctcac tagctagtaa
     1201 tgccactcca gctccgtcac tcgtctgaga actatttcct gcagtcactg tcccgtttaa
     1261 agcaaatact ggttttaatt tagaaatcgc ttcgtaagat gtgtctgctc ttgggccttc
     1321 gtccttattt atcaattttt tctttatttc aacctttgaa gttttcagat cagggtatct
     1381 tatagatatt tcgattggac ttatttcagc gttgaaatcg agtctttcct gcgcagcaat
     1441 tgctttttta tggctttcta tggcgaattc atcctgttgt tctcgagtaa tttcccagcg
     1501 cttggcaacg ttttctgctg taatccccat gccataagct atagcaacgt gctcatcatt
     1561 aaaaatagat ggattcgccg tgtatttatt tccacccata ggtaccatgg acatgctttc
     1621 gacaccgcca cctagtgcta aatgagcctc ctgattacgg attgtatctg ctgcagtaac
     1681 tatactttgt atgccagatg agcaaaaacg attaatagtc attgctggaa cagattgtgg
     1741 aagatcagcc aataaagaag cgattcttgc tacattcata ccttgttccg cttctggcat
     1801 tgcacaacct attactacgt cagctatctc ttgccaatca atgttttgat gtctatgtat
     1861 taaacattta atagtatgag ccaataaatc atcgggtaat gtgtttttaa aaacacctct
     1921 tggtgcttta cctactggtg tacgtaatac gtcaactaca tatacgtcag tcatcctaat
     1981 tctccttgct tagttgcgca gtggtttacc agtctctagc agatgactga ttctggcttg
     2041 ggttaatgat gtattggcta attttatgaa tgcttctctt tccaacttta gcatccattg
     2101 ttcatcaacc aaggttccat gattgacatc cccaccacac aacactttcg ccaattcatt
     2161 cgccagaaaa taatcatgct gtgatataaa tccaccttcc aaccaattta ctaaaccgga
     2221 ttgtaatcgg gcatgcccct ctatacctgc cactttaaat cgttgttttt tcataggagg
     2281 aagataatta gcagctcgca tggctttgat tttttctaaa gaggcatata aaacctcatc
     2341 agcatgcatt agaatagaat cagccgcgcg cagatatccc atatttctgg cttccaccgc
     2401 acttgcagct acttttgcag ttgctatttg ctgaaaaact ggcaatataa aagttaataa
     2461 atcagcttgt tgggcatgat gtgccgaacg catagcgagc tctttgcaac caccaccagc
     2521 aggtataact cctactccag cctcaactaa accgggataa gattcaaaag cagcaacaat
     2581 agaatcacaa tgcatcatta attcacaacc acctcctaat gctcgacctc ttaaagcagc
     2641 tacagttgga attgaactat attttaagcg cattgctacc cgttgaaatt gctctaccat
     2701 atgctcaaga gcttgtattg ttccatctgt taataatttg aagacgccac gtagatcagc
     2761 tcctgaacta aaattggatg catcttgttg ataaataatt aatccctggt aatccctttc
     2821 tgcgatatcc aaagcagtct ctatgccatc gagaactgat tgaccaattg tattggcttt
     2881 gcttttaaaa ctaatcacag ccacatcatc ttttaagtac cacaaacaaa caccctcatt
     2941 ttcataaata acatgaacag gatgatgtga ctcctttaat accctgtcat ggaagaattg
     3001 tttatggtaa acaggtaact gacttctggc gtgataattg tttttaccag gcgaataagc
     3061 gccttctcgt gtgtaaaatt cctttatttc atataaccac tcaggtagtt ttgcgcttga
     3121 taatgccgta ttatcctgta ttgattcatc gatttgtcga gtcacactgt gtaaatcaga
     3181 aagctgccaa gtttcaaatg gtccttgcgc ccatccaaat ccccagcgaa ttgccagatc
     3241 aacatctctt gcattatcag caatttcttc caaatgatac gcacagtaat ggaataaatc
     3301 tctaaagcaa gccgccaaaa attgagcttg cttattattt gatgtcatta aattcttcat
     3361 tcgagcttca ggatctttaa tatccattat cgctttcagt tcatcactcg cttctgctcg
     3421 agagggtcta tattgaccgg tttgaatatc ataaacttct attactttac ctgcttttcg
     3481 gtaaattcct tggccagatt tttgtcctaa atgaccttcc ttaattaaac cattcaacca
     3541 gtccggcaga ataaacattt tatgccatgg atcaccagtt aattgagact gcatggtatt
     3601 aataacgtgt tgcatagtat ccaaaccaac cacatccatg gtacgaaaag ttgcagattt
     3661 cggtcttcct aataatggac cagttaaggc atccacctca tcaattccca tattcatggc
     3721 caaagtatga tgcaaggtag ttaatagtga aaagactcct attcgatttg cgataaaatt
     3781 aggagtgtct ttcgctctca caactccttt gcccaaatac tcggttaacc atgtttccaa
     3841 ataatcgagc aaatcagatg atgtggtatt ggcaggaatt agttctgcca aatgcatgta
     3901 acgcggtgga ttaaaaaaat ggactccaca aaaattggat ctatgctgcg caggtaatac
     3961 ctcacatagc ttattgatac ttagccctga tgtattacta actaaaattg ttttatcaga
     4021 taaataaggc gaaattcgct tgtataagtc ctctttccaa tctatacgtt ccgctatcgc
     4081 ctctatgact aaatcacaag aagataactc ttccaaattt tccgcataat tttttgcttg
     4141 taataactca ttggtttgag gagttgcaag aggagttggt tttagtttac ctaaattggc
     4201 aatagcttta tcgaccaagc tgtttttagg cccttctttg ccagctaaat caaacagtaa
     4261 agtctctatt ccggcattaa cgcagtgtgc tgcaatttga gcacccatga cgccagcccc
     4321 taaaaccgca acttttttaa tgaaaaaagg ctcctgcatt gttattatcc tcttgttaat
     4381 aatcgattga tgcctctatg agtgagattt acaggtaaca ggcacttacc attggctaaa
     4441 aggagcaata atctgttgat cttactcctt tctacatctt aacttaaatt aaaacgaata
     4501 ccctgagcca aaggcaattc atctccccaa ttaattgtat tggtttgtct cctcatataa
     4561 gctttccagc tatctgaacc tgattcacgt ccgccaccag tttctttttc gcctccgaaa
     4621 gcacctccaa tttcagcccc tgaagtgcca atattaatat tggcaatacc acaatcactt
     4681 ccacaagcgc tgagaaagag ctctgcattt tttaagtttt gggtgaataa ggctgaagaa
     4741 agcccttgag gaacaccatt atgtaaagca atagcctcat ccagtgtccg ataggacata
     4801 acatatagaa taggagcaaa agtctcttcc tgcacaatat cccaatcgtt ttttacatca
     4861 caaactaaag taggctgcac aaaggagcca gcttgtttta atatttctcc accataaaca
     4921 atttgacctc ctgccgcttt gattctattt attgcttttt taaactgttc aacagcttgc
     4981 tggtcaatca aaggacccat taaatttcgt gtatcgagtg ggtcaccaat agttatctgt
     5041 tcataagcat gtcttagcct tttgataaca tcctggtatt ttgattcatg aacaaacagg
     5101 cgtctcgtag tagtacaacg ttggccagca gtacccacag caccgaatac tatggcagga
     5161 atagccaagt tcaaatcagc agactcatcg aggatgatgc cgttattacc tcccaactcc
     5221 agaatgcttt tacccagtct ggcagcgact tttgctgcta cttgctttcc aactgcagta
     5281 gagcctgtaa aggatattaa ctgtattcgt ttatcatcaa ccatggcttc aaccacatcg
     5341 tgagaattgg gaatgatcaa gccaaatatt tcaggacaat tattttcttt caatacctga
     5401 ttgcaaatat gttgtactgc cactgcacag agaggtgttt ttgccgatgg cttccatata
     5461 gtaacgttgc cacaaatagc agataaaaat gcattccatg accatacagc aacaggaaag
     5521 ttaaatgctg aaatcacccc aactattccg tacggatgcc attgctcata cattcggtga
     5581 tttgggcgtt ctgagtgcat ggaattacca tacaacatac gcgattgccc tacagcaaaa
     5641 tcagcgatat caatcatttc ctgaacttcg ccatcacctt cctgtttgga tttacccatt
     5701 tccagcgata ccagacttcc caagctatct ttattttctc ttagtgcttg ccctatttgc
     5761 ctgatgattt caccacgctt tggagctggc acttttcgcc aggcttgcgc tgcatgctcc
     5821 gctcgctgca taacctgctc ataatcatcc atagtgcaag ttgctatttc agcaagctta
     5881 ttcccagtac tgggattgaa ggattccaga gtatgggcat ggttgtcact atgccaacca
     5941 tgtccactaa aggctccggg gtttactgat ttaatattta aacgttgcaa taaatccata
     6001 agattctcct aggcataata tttgccgaag cgattttcca gaacgtggga caatttaaac
     6061 ttttcttgca aaataagccc tttatattca tttccctgtc ccaataccaa atcaacaaca
     6121 gcgcatacgc ccgaagctgt actgacctga atagctgacc actccaaacc tcgaattact
     6181 tcagggtaaa ttttcttgac ataacttttt tcagttagtt cgccctgctt aataccctca
     6241 acagtaacat aaactatcac gatatcctgg tatgtttttg gaattgcatt ttccagtatt
     6301 ctttttaacg tccctcgatc ttcatttaat cgtaagtcat tcattaaaaa acgcattttt
     6361 ttacaatggc ctggatagcg catggttttg taattcatcg attgtatttt tccagcataa
     6421 agttcagcca aacttcccaa accaccggac gtgttgaacg cttcatattc acaaccatca
     6481 atctgaattg actccaatcc ttccagtgga gccataacca catctttacc accttcaata
     6541 ccataacaag gattaccata ttcattaatt accccatcag tagaccaggt taatgaataa
     6601 tgaagcgcat tattagcgcg ctgtggcaat gctccaactc ttagtctggc atgatgacat
     6661 ttctcaaatt cctgcatcaa actattggcc gcaatactga taaagccagg agctaatcca
     6721 cattgaggaa caaaagctgt ctcggcattt tgagcaatag cttttactgc ctccgttaca
     6781 gaggtatctt ccgttaaatc aaaataatga gctttggcag ctttggcggc tttggcaaca
     6841 tgagtattta aaaaataggg tagactcgaa atcactgcga taatattatg cttttgcaaa
     6901 taggcctgag tggattgttc atctttaaca tccaatgcaa ccgttttaat ctcaggcagc
     6961 gcagttaata atcttgtcac atcagagcca ttaaattcca gatcggccaa atgaacctga
     7021 taagacccac tatcggcgag tagacaagca attagactgc ctattttccc agcaccagta
     7081 atcattacgt tgtacataca atccttctcc tttgtaaaac ttgataatcc tacacaaatt
     7141 atcagagatt agatagatcc agaacatgat caatacagca attcttgata tcacttgacc
     7201 aattagtctt actcatttat cttgaccact ttaatcagta tagttgtgac atgaagcagg
     7261 ccgctttgat acattcgaca ataataaaca catcaacact aaaccaatat tcgaagttca
     7321 ttgttgtatt tttagcaggt ttattagccc ctttgggatt tgcacctttc catctgccag
     7381 gattaactat tttgagtctg gccattttat tttctaaatt tcttcaatgc acagttaagc
     7441 agggatttac tcttggtttc ctatacgggt taggatactt tggcttcgga gtatcatggg
     7501 tgattatcag catccacgac tatggacaac ttgattactt tcttgctgga gttatcacct
     7561 tattatttat catgtatctt tctctgtttc ctggaatagt cggttattct tttaaactcc
     7621 tttcagccaa gtgtaattta ttaacatgca tgcttttgtt tagcactctt tggtgtatag
     7681 gtgagctcat ccgttccaag ctttttactg gattcccctg gctattaaca ggtataaccc
     7741 aaatcgatac accattgaga tatatggcac cagtagttgg tgtttatggg cttggattaa
     7801 tatgttcttt tttgggtacc ctactggttc tgataattaa agacaatacg attaaacgat
     7861 attactacct tagcgttttt accttgattc ttattgcacc atccctgggg aaaaatatcc
     7921 attggacaga aatcaagaaa gaacctattt ctgttggtgc aattcagcct aatctctcaa
     7981 tgagagacaa gtgggacgaa actctctttt gggatttatt aaaatactat gaaaacaaaa
     8041 cagataaact gttaggaaaa aatttaattg ttttacccga atcagccata cccctaccag
     8101 caagttatgt tgaagattac ctacagaaac tgcatgaaaa agcattaaaa gccaaaagca
     8161 gtttaatttt gggaatcttg caacctacag ataatgatga aaccaatttt tacaactcta
     8221 tcattagctt ggggaaagct acaggggcct atgtgaaacg ccaattggta ccatttggag
     8281 aatatatacc aaaacccttt atccccatca atcgttggtt aaatcttcca gaacctaatg
     8341 ttgttcccgg cagatcgaat cagccattaa ttaacatagc taaccatccc attgccagtc
     8401 taatttgcta tgaaattgct tacccaaata tcttgagatc acaaatgcct gctgctcaat
     8461 ggatagtctc tatcagtgat aatggctggt ttggccattc cctggcaagc taccagcaat
     8521 tacaaatggc tcaaatgctc tctttgctta caggacgata ccaaatcctg gtgaataatg
     8581 atggactctc ctcagtaatt aaccaccaag gaactattgt cgaagggcta ccagctttta
     8641 attcaggaat gctccaaaca gatatttttc ctgcaaatgg catcactccc tggataattt
     8701 ggagtgacta tcccgctttt attttttgtt tcatatttgt tctttttgtt ttgtttttta
     8761 atattaaacg atttacagaa aaataatcta ttgctggcaa aggcaagagg ggttatcctt
     8821 agcagttccg tgttattaaa tttattggcc tatggataat acctataacc cccaagaagt
     8881 tgaagaacaa gcacaacaat attggcataa gaagcaatca tttaatgtta ctgaagattt
     8941 aaacaaagaa aagttttatt gcttatccat gtttccatat cccagtggca cattgcatat
     9001 ggggcatgtg agaaactata ctctcggtga tgtcatagcc cgatatcagc gggcattggg
     9061 aaaaaatgtc ctgcaaccta taggatggga tgcttttggc ctaccagccg aaaatgcagc
     9121 catcaagaat aaaattcctc ctgcagaatg gaccaggaaa aatattgctg ccatgaagga
     9181 gcagttttta cgcttgggaa atgcttatga ctggaaaaga gaaattacta cctgtgatcc
     9241 tgaatattat cgctgggagc aatggttttt tattcgttta tttgaaaagg gcttagttta
     9301 caagaaaaac gctgttgtta actgggatcc tgttgatcaa acagtattag ctaatgaaca
     9361 ggtagttgac ggtcgtggct ggcgttcagg tgctttggtt gagcgcaagg aaatttctca
     9421 gtggtttatt aaaattacct cctatgctga tgagctttta agctctttag atagcctgga
     9481 tgaatggcct gcacaagtaa aacaaatgca gcgcaattgg attgggaaat ccataggcac
     9541 agaaatttac ttcaatgtaa acaattatcc caaacgattg aaaatttaca ctacccgccc
     9601 agatacattg atgggagcaa cctatcttgc tgtggcaaca gatcatccat tagcaaaaga
     9661 agcggcttcc aacaataaaa aggttcaaga attcctcgat agttgtcaag gaattaagat
     9721 agctgaagca gaactggcaa ctatggaaaa acgagggatt gacactggaa tgactgcaat
     9781 ccaccctata acgggtaagg agttacccat atgggttgct aattttgttt taatgcaata
     9841 tggttctggt gcagtcatgg cagttcctgc acatgatcag cgcgattggg agtttgcaca
     9901 aaaataccaa ttgcctgtca aacaggtaat caagcctata gacattgagc atgactttaa
     9961 tcaatcagct tatactgaag aaggaattct gattaactca aatcagtttg ataatctact
    10021 ctctagtaaa gccattcaag tcatcacaaa ctttctggaa gaaaatgacg ctggtaaagc
    10081 taccattaat taccgtttaa gagattgggg cgtttccagg caaagatact ggggaacccc
    10141 tatccccatg atcatctgtg agcaatgtgg tattgttcca gtccctgatg aggaattacc
    10201 tgttgttctg ccagagaatg ttgattttac cggtaccggc tctccactta cacaatgcaa
    10261 agagtttgtt aatgtgacat gtccaaaatg cggtcaagat gcaaatcgag aaacagatac
    10321 atttgatacc tttgtcgaat cttcatggta ctatgctcgc tttgcttgca aaggtcagga
    10381 aaatgccatg cttgatgacc gagctaaata ttggacacca gtagaccaat acatcggtgg
    10441 aattgaacat gcagtgatgc atctgctata tgctcgattt tttcataaat tgatgcgcga
    10501 tgaagggtta gttaattcgg atgaaccctt taaggcttta ttaactcaag gtatggtttt
    10561 aaaagacggg cataaaatgt caaaatcatt aggtaatgtg gttgatccaa accatcttat
    10621 caatacctac ggggctgata ccgctcgctt atttgtcatg tttgcatccc ctcccgagca
    10681 atcactggaa tggtcagatt ctggagtcga aggagcacac cgctttttga aacgagtttg
    10741 ggcgttttca catcagcata gagacatgct cattgacatt aacgatagta ttttgagtgg
    10801 aaatggtcat gtcgactgga aggaagcgga gtctcgatta aaaaaatccc gtcatattgt
    10861 gcatcaaata cttgctcagg caacccatga ctatgaccga aatcaattta acacggttgt
    10921 ttctggctgt atgaaattat ttaatgaaat atctgattac tccattgaaa ctgaaaatga
    10981 taaatttttt atacactcaa gcatcagtat attgctgaga ttactcgcgc caattacccc
    11041 gcatatttgt cattgccttt ggcagcaatt aggttttgat aaagcaataa ttgatgcgcc
    11101 gtggccaaag gtggataaaa gtgctctaaa aactgatgaa gtagattatg ttgtacaggt
    11161 taatggaaag ctcagagctc aatttactgc aagcactgat gcgagtgaag aggaattaat
    11221 tgctgcagct aaggagcatg cccacaactt tgtggtaaac cacaccatta aaaaagcaat
    11281 tattgtccca cataggcaac taatcaactt ggtcattgga taaacatgtg tgattcgtcc
    11341 cagcaatctc taaatatttt acacaatttc tcaagttatg ggtggtatta cctgtaagtg
    11401 caaataagag agggaaaaga agaatgactc agatccacaa gtacttagca tctcttatac
    11461 tgatttggct gctaactggt tgtggttttc atttgcgtgg agcaattgat atccctgagt
    11521 ggcttgataa tgtttctatt atttccaggg atggaaataa agaactggtt tctatactca
    11581 aatcccagct ggaaggctat aaaattgatg tcaattctga cccatctctc gcgaaatact
    11641 ggttaattat taacagttcc agtttacagc agcaaattgt tagtattggt gcaagtacca
    11701 atccaagaca atatcaatta atcttaacca ttgaatttat gttgcagact cccaaagggc
    11761 aaattattaa aacccccaga aaagttaatg tgagccgtca actcaccgtg aataatgaca
    11821 ggatactagg gtcaaatgaa gaacaagctc ttttagtcag cgaaatgagg cgtgatgccg
    11881 ctattcaaat cattaatcgt ttaaaccgac catgatatgc aaataaatca acaagctctt
    11941 actcagcttt tacaaaaaaa aatacatcat cttaatgtac taattggaca agatcattat
    12001 cttattgacg aagcactcaa taacataaaa tcaatgctta aaaaatcatc tgaatgtgat
    12061 gagaaaatcc tctctattca gtcccctgaa gaatggcatg tcgttctgga agaaacaaat
    12121 aattactctc ttttttatca aacagttatt ttaaccattt tctttgataa aaaaacgata
    12181 gatacaatgg gaaaaaaaat tcttagtgaa tatctcaaat caaccaactc tcatagttat
    12241 attattatca gagcccctaa ccttaccgcc aagcaacttc aatggttaac acatgatgaa
    12301 caagcgctga ttatcattgc acaaccatta agctcagaag caatgaagag atggattaat
    12361 gagcaattaa aattaaatac tatctctttt gatccatatg ttcctgaatt aatttatcaa
    12421 tatactaaag gaaatatgct agctacggct caggtaattg aaaaaattac tttgagcaat
    12481 acacccggca ctctcattac tccttacctg gccttggaac atttatcgga tcaatgtgat
    12541 ttttcccttt ttgaactgat tgattcatgc ctacttggtc atagcgatag agcaattcat
    12601 attttaagac aagctgccaa taataaaaca gaagtcacat tggtattatg gatgcttacc
    12661 caagaaataa gaacgctatt acaattatta tttctaatgc aacaaaagat ggatatcaaa
    12721 atggcatcta atcaattaaa aatttggact caaagagtta atctttatca atccgctgtt
    12781 aaaagattaa atactttact tttacagcaa cttcatcact attgccaatc tattgatgat
    12841 aaaatcaaat ccaactcaag caatcaagca tggaatgctc tagagaacat ttctctttcc
    12901 ttatgtttag ggcaattact aggtgatgca tgcatagcat agcaatattt ggtggaacat
    12961 ttgacccggt gcataatggt catatcaaga ccagcttggc aattcaagct aactttggct
    13021 ttgattccta ttatttttta ccttgtaaaa gccccgcaat caaacctccg tcctttgcca
    13081 gcagtgaaca aagagtagaa atgctaaagt tagcgctcaa accttaccct gattttaaaa
    13141 tagatactcg agaactggat cgagataccc cttcttatat ggtatacact ttgcaaagtt
    13201 tcagacagga atataccgac tcatcattaa ctctaattat tggatacgat ggattattaa
    13261 atctaccaca atggtaccaa tgggaaaaaa taatatcctt agccaattta cttgttatca
    13321 atagagaaga gttcttccaa cagcctgtgc caaagtcagt gcaaactcta cttaaccaat
    13381 atagaaatga tgataaaaat atactgctaa atcatcatgc tggtagtatc tgcttataca
    13441 atgctggaca ctatgatata tcctcaacaa aaattagaga acaattaaaa caacataaag
    13501 atgtaaagag taatttgcct gatttggtat acgattacat taagaagcag gaactgtatc
    13561 aataatattt tctgttttat caacagtctt gacctgcttt ataaattcag gcaaatggct
    13621 atatagcaaa gctacaacag gatcaaatcg tgcataaagt ttggactttt gtgaatattc
    13681 ctgataaggc aaagaggtca ttttaacagc aaccataatc agggcaacga taaaaacgcc
    13741 acgtaaaaag ccaaaaccca ttcctaaagt tcgatcagtt ccgcttaaac cagcccgttt
    13801 taagataaaa ctgagtattg cattcactac ccccccaaca aaaagggtgg caaaaagaat
    13861 aataataaat ccaatagccg ttcgtactga ctgttcctcg atataagaac ttaaccaggg
    13921 atccaggctt tgtgaataat taaaacctag ccaaatagct aatatccaca cacataatgc
    13981 aacaagttcc tttacaaaac cacgaaacaa tccggtcaat acggataaag cgataattgc
    14041 aacaattgct atatcaaccc attgtagttg cataactaac tccagtattc acaacaaaac
    14101 catttaattg catggcacta gctaattgag tttttaattt tattacatct tttttaacgg
    14161 gcgaatggcc aacaactact ttataaacta ctccatttct gctattggac ttaagataat
    14221 tcgccttgta gcctttgctg cgcaatcgat tgactaaagc ttgagcatta gctatttgtg
    14281 taaatgaagc cagctgaaca gcataaatat cttgtttaac tgctggtttt ctcgcagcaa
    14341 caatttggct gacctttttg ttagtattaa cctttgccgt aacaactgga gatcttttcg
    14401 ccggtggttt tgctgcagcc agggtcatta ctttttttgc tgtcgcattc gccgctttat
    14461 ttactgccaa tttaattggc tctgatttaa ttgaattctc tctatccgct tcaagcgtcg
    14521 cgattttaga cgttctgttg agattgtctt cttctgtgct gatgggttct gcctttacca
    14581 gactgaccga ttgtttttca acaggtattg gggggatttc tgtcttagcc acttttatag
    14641 ttttaaatac ctctttttca tcagtaataa ctacatcagg aggcaatggc tttggtggta
    14701 atttcacatt gacgctaaaa ttactttcag ggctttggct ggattttttt attaatgcag
    14761 gagcaaaaat tgcccccaat gaaataataa cggccaaacc tatcaagcga tgtttaactt
    14821 tctcatccat tatcagtttc attgattctc cttctgctcc aaaagattat gttgggcagc
    14881 cattacatga ctaaccgtaa aaaatgaacc atagacaata attaaatcgt catctgatgc
    14941 ttgctttaat gccttttcaa aagcagcaaa tgggtcatta tagcaaacat ctgcaaaaat
    15001 ttctgcttcc ctgaatacag aaagcaattc ctcttgactt gcagcgcgct tattatgtaa
    15061 ttgtgcagga taccatcgat taacacaatc ctttaaaggc ataatcaatc cataaagatc
    15121 tttatccttt aaagcggaga aaacggcatg aatttgccct tttacattga ttttttttac
    15181 tgtatctgca agtaatcgaa ctgactgcgg attatgagat acatcgtaca aaatatcagt
    15241 ttttcctttt tgtaattgca atcgccccgg gatatcagtg agatccatcg ctttctttag
    15301 gttctcatag gatacaggca aatcattttg catcaaatga caagccatta cagctgctgc
    15361 cgcagaacga agttgaattt ttggcctggt caattgctca aatggcttac cttgacaagt
    15421 aaccccccaa tggtcagctt cctcctgata aaaatagtct ttaccgtata aataagataa
    15481 tgctccaagt tgtaccgcaa cctcaacgac actttttggt agtgagagat ccgcgtagat
    15541 aaaaggttta ttttttcgta gtattccagc tttctcatac cctatcaact caagtgtatc
    15601 accaagaaaa ttctgatgat cgaagtcgat agtagtaatg atcgctaaat cagtatcaac
    15661 aatgtttgta gcatccaacc gtcctcccaa gcctacttca agaattatca gatccagatc
    15721 atgctgtttg aaataccata aggcagcaag tgttgccatt tcaaaatagg ttaaattggt
    15781 agggtaacgt gcttcttcaa tcacagaaaa agccttacat aaatctttat cttgaatcgg
    15841 tgctaaatta attcgaatac gttcattaaa cacaagaaga tggggcgacg tataggtacc
    15901 aactctaaaa cctgactggt gatagatggt ttcaagtgct gtgactgtag aacctttacc
    15961 gtttgttcct gcaactgtta ttaccttgca atttggtcgc tgcaaattta atttctgcgc
    16021 tacagtcaaa actctggtca aaccaagctg tatttcctgg gtatttctgg tttccaaatg
    16081 agtcaaccag tgatttaaat cccattcact ccatggcgtg tgcaaaaaaa ctctccgctc
    16141 ctttatgagt caatttggaa accaattcgg atatagttgc tcgaagatgt ttacgttcaa
    16201 ctatcatatc aatatgccca tgttccagca aaaactcact tcgttgaaag ccttctggca
    16261 aagtttgtct aaccgtttgc tcaatcactc ttggcccagc aaaaccaatt aaagcattgg
    16321 gttcagcaat gattatatca ccaagactgc caaaactagc tgatactcca cccatcgttg
    16381 gatcggttaa cacaacaata aaaggcaacc cggtttcagc aaattttgct aacgctgctg
    16441 atgttttagc catttgcatt aaagaaaata atccctcctg catacgagca ccgccgctgg
    16501 cagtaaaaca aatgtatggc cgcttttctg ttgcagcaac attcactgcc cttacaaatt
    16561 tttctccaac cgtcgccccc atggagccgc ccataaaatt aaactcaaag gcactgacaa
    16621 ccactggttg ttgcattaaa aaccctttaa caacaattaa tgcttccttc tctcctgtcg
    16681 ctttttgcgc ttgagaaatt ctatccttgt attttttgga gtccctgaat tttaatctgt
    16741 cctgtggctc cagcccagcg gccacttctt cttgccctga ctcatctaaa aattgagcaa
    16801 ttctcaagcg cgcggaaatg cgatgatgaa aattacaaga tgggcaaacc ataaagtttt
    16861 tcgccagttc ggtactgtaa agcacctcat tacaaccaga acatttaacc cataaacctt
    16921 ccggaacccc cttcttttga gaggtctctg ttcttattct tgatggtaac agttttttaa
    16981 gccagctcat aaattagaac ccagatatta tcaaatcgga ttattataca cagaaaaaaa
    17041 taatctgaca tgcctatatt ggacttgaat ttcacataag ggaataagat attttaccaa
    17101 atcggcttga caatcagaac caaatcaccc tgaaaacccc aattactctt taaaatattc
    17161 aagtccatct gagcgatttt agacgcattg attatatttt gtcgatcttt ctatgtattt
    17221 aacaccctaa ttttattcaa taattttttg cgatttttca aaaaagcgct tttggaacta
    17281 aagttttttt cactatttcc gataactaaa tcgggtacag taaattcaca acaataaatc
    17341 gaatttactg aattacaaat gtcccatacc agggattcag gtctgaggag actaaaacta
    17401 tggctcaagt aatcaacact aatgtggctt cgcttacagc ccaacgtaat ttaggtgttt
    17461 cgggcaacat gatgcagaca tcaatccagc gtttatcatc aggattaaga attaacagtg
    17521 ctaaagatga tgcagcagga ctcgccattt ctcaacgaat gactgcacaa attcgcggga
    17581 tgaatcaagc agttagaaat gctaacgatg gtatttctct ggctcaggtt gctgaaggag
    17641 ctatgcagga aacaaccaat attttgcagc gtatgcgtga gctttccgtt caagctgcta
    17701 actcaaccaa taactcatct gaccgggctt caattcaaag cgaaatttca caattaaaaa
    17761 gtgaattgga gcgtattgct caaaatactg aattcaatgg tcaaagaatt ctcgatggct
    17821 ctttctctgg tgcaagcttc caggttggag cgaattcaaa ccaaaccatc aatttcagta
    17881 tcggcagcat aaaagcttct tccattggtg gtattgccac ggcaacagga acagaagtag
    17941 ctggtgcagc agcgacagat atcactatcg caattggagg aggagcagca accagtatta
    18001 actcttctgc caattttacc ggggcactaa acgggcaaga tgctacgtct gcctatgcca
    18061 aagcagcggc tatcaacgat gccgggatag gaggactatc agttacagca tctaccagcg
    18121 gcacacaagc agttggcgca ataggtggaa ccgcaggtga tacctacaac ttaacgatta
    18181 atggtgttgc aatatatact aaccttaacg tagcaacggc attaaccaac tccgatcttc
    18241 gggatgccat caatggtgtc agtaaccaaa ctggagttgt cgcgtcactt aacggaggta
    18301 atatgacact cactgctgct gatggcagaa acattacggt aacagaaagc ggtacaggtt
    18361 ttactgcagg tactgacggt ttaactgtga ctggtggcgc atttgatggc gcgctacgcg
    18421 gtacattatc aatcagcgcc gttgatacta ttgcaatcgg tggtactgtt gccaacgtgg
    18481 gtttaagtgc caatatcgcc aaagatacag taggtgtaga ttcactggat gtgagtacgg
    18541 cggctggtgc tcaaacagcg attaagagaa ttgatgcagc acttaatagt gtcaactcta
    18601 atcgggctaa tatgggtgct ttgcaaaacc gctttgaatc tactatagct aacctgcaaa
    18661 acgtatcaga caacttatca gctgcaagaa gtcgtattca agatgctgat tacgctgctg
    18721 aaatggccag tttgactaaa aaccaaattc tgcaacaagc aggtacagcg atgttggcac
    18781 aagctaatag cctaccgcaa tctgtattat cgttgttagg tcgataggat gtcgcaatcg
    18841 aagtgcagct gttgctgcac ttcgatttct ttatacctcg gttgatgggt ataaaaagga
    18901 gtaagaaaaa tgagtattga acctgtaccc tcagtcaata gggcagctca aacagatcaa
    18961 acaaaaatag ttgaggaagt ctctaagcca gaaattaaaa atatggttta tgattccgct
    19021 ttggatgccg aaacaaaaca ggcgctggat aaagcaacag gtttgcttca aactataata
    19081 acagagaaaa tatccgataa agtattgcgt aaaatgcctt ctgatgaata tttgcaatta
    19141 ctaagcttgc ttgatgatat tattagtggt tctatagaca agcacgttta ataatttgaa
    19201 atatttatta aaaatttgac tagaacaata acaacataga aaagggatac tctatggaac
    19261 tctcttctcc aggcgtaggg tcaggacttg atgtaaaaaa cctggtcgat gcgatagtca
    19321 cggcagaaat aactccatcg caagtacgtc atgataaaca gttaaatagt gtcaatacag
    19381 agttgtcggc gcttggtcaa ttaaaaagct atttaactaa tttgcaaact tcgttagcca
    19441 agctctccag tcttagccaa ttctacacta tgaaatcaac cattagtgat ccgaattact
    19501 tttccgctac tctggggact ggcgcgacta aaaatactta tcaaattgaa atacaaaaac
    19561 tggcacaaca acacagtctg gcaagctctt acatgactaa tactggaagc ggaacaataa
    19621 ccatagattt tggaacctat aacagtgata aaactacttt tacaccgaat ccttctgcta
    19681 cgtcagtcaa cataaccatt gcgcctggca acgatagcct tgttgcggta cgagatgcca
    19741 ttaataatac taattcagga ataacagcca gcatagttca agatagccag ggctccaggc
    19801 taactatcac ttcgtcaaaa accggcgaaa attatgccat gaaaattagt ggtggagttg
    19861 cctcattgaa ttatgatcct actatgggag tgaattcatt aactgaaaca gtagcggcac
    19921 aaaacagtac tgttaaaatt aacggattaa ctttggttca aagttctaat caattgaatg
    19981 aggcaataac tggtgtcacc attgatctaa agaaagcaga gattggaaaa attattacct
    20041 taactgttga tgataataaa gatcagctca caggcttaat caacgagttc ataaaacaat
    20101 acaatgacac catgactttt ttaaccaact taactggtta taacaatgaa accaaacaag
    20161 gaggagtatt tcagggcgac cctcaatttc gcaatttaaa actcaattta aacaagtggg
    20221 caactacccc tttagctaac caggaaggac caatccgctc attagccgat ttgggaatca
    20281 tcaccaataa acaaggttta ctggaaatca aacaaaacaa gtttaaaact gcactcgata
    20341 atcactataa agaaatagga gcattattcg caaagactgc gaagactaat gatactggcg
    20401 taaacatcaa atccattaat tcaactgtca aatcaggtac ctatgatgtg gtgttgactg
    20461 aatttactcc tggagtcagc atgtctggaa caataggagg cttatctgct acatcatccg
    20521 atggcattac attgacagga agtggtgatt taagtggtct gtcgattaat gtattttcag
    20581 ggtctgcagg ctcccgtgga caaataactg ttacagacgg tattgcaatc caattaagtt
    20641 ccttgctgga tgcttacatc gaaactaaag gcgatttgga gcagagaaaa gatcaactca
    20701 ataatcaagt caagcaattg aataaaatac aaaacgaaat tgatatcagg cgcaaatcaa
    20761 ttgaaactcg ctatttaaaa caattcactg ctctggacac tctactcagt caattacaaa
    20821 gcactagtgt ttttctatcc cagcaattag ccaatttacc ccaattaaaa ttgaaataat
    20881 aggaggtaat gagaatgaaa aacctatatt cacaaatttc tagccaatac aaagccatag
    20941 agctacaaac tcgcatagaa acggcctccc ctcatgaact tatagattta ttacttcaag
    21001 gagccagatc tcacattgct actgctcagg gtaatataca gcgtaaccaa attaaagaaa
    21061 aaggagaaca cattagcaaa gctattagca tcattgaagg tttaaaaatg agtctcaatc
    21121 atgataaagg tggcgaaatt gccgagaatt tactccagtt atatgattat gttcaacaaa
    21181 tactacttaa agccaattta aaaaatgatg aagatttatt agctcagtcc aatatgctat
    21241 tagctgaagt gcatcaagcc tggcaagcga ttaactctaa tgctggtaat gaactttaat
    21301 atttgcaatg agctcccatg attcagcatt tgctttttac ggcttgataa caaccaaaat
    21361 tagagtcttc ttggcttggc aattattaaa cccagcccat ctatttttat tctcccaatg
    21421 caaatagttt aaggcttcca aatatcaata agatatgagg aattgtctgg aagtcttgta
    21481 gttatcgaaa tttttgtcca ttttggtaat caagaatagg attataattt tggcaaattt
    21541 gtatagattt atcgaattgg aatataaagt ttttccatta tatgccgcca aaacagttag
    21601 gatgaataaa ttaatttcat tatgaataca tttaaatcat cactggcatc tgtaatttgt
    21661 ctcttactta gcatgcaatc ggcacaggcg caaatgcgat atggagaaac tctctgcaac
    21721 ttgcctgaat atttttgcat gaaaataaaa agcggggaca gttgggatag gcttttccct
    21781 aacatcgaag agcaagatat tgtcagacgc attaaccgaa tgaatatcag gctcaaaccc
    21841 gggatgatta ttgctgttcc caaaaatata gagcgtttaa ccatctatga cgtctcgcct
    21901 tttccaagat atattgaacc aagtggtgaa aaaacaattt atgtcagtca aaaaaaattg
    21961 gcatggggag cttatgatga agacggtgaa ttgctttggt ggggaccaat atcatcaggt
    22021 gttggaaaat gtaaagtaat aggtggttgt tctacaccaa caggttcatt tcgcattatc
    22081 cgcaaacaag atattgattg tgtttcaact gttttcccaa gaagggcaaa tggcgaacat
    22141 ggtggtgcac tgatgcctta ttgcatgcac ttttttagag gttatgcttt gcatggaagt
    22201 tatgaagttc caggatatag ggccagccac ggctgtgttc gcatgtttat tgaagatgca
    22261 cgttggttaa atgaggaatt tatcgacctc ccaggaggag gaatgagagg aacacgtgta
    22321 atccttgatg cgccagatgc tcaagagaat cccgataacc tacaataata tattgaaaac
    22381 acaatcaaaa aactcacccc caatagacaa atagtggtca acctgataat attttattta
    22441 gtgaattttg ccatgaggtg tgttaacgta taaattcact gtgtgaataa
//
LOCUS       DAXLDT010000044        22429 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXLDT010000044 DAXLDT010000000
VERSION     DAXLDT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22429
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.044"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            86..1828
                     /locus_tag="ACF2SP_001310"
     CDS             86..1828
                     /locus_tag="ACF2SP_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2359103.1"
                     /translation="MSFSKISKTLENSATLIDEKAEQDASDLSHTLGSDSQLLISEES
                     SDVLLENTGENLVQQACIDGNLEKLKELLRGDMWGMDEVSLNQVNNRGETLLMLAIKY
                     GHKQMALFLARSLFHLHKIDNSGKSALSLAHEKGWEDIVQLILESINNREARIRELHY
                     AAIHGYVESVSQLLGIIDKYGFKPHGFILVGDETPRFENIFHAACCGGSLEIVKLLIE
                     HGADINSKSQFSENNYSHYPATPILVACRYGHANIVEYLLAQDGIEYDINELLHTAVG
                     FGHFAVLDLLLRHSKENSTRNLNIVGEYPLTHFSATLLTRACYSGQVDIVDRLIAEDI
                     DMSNADNRQAFWTAIFCAKTQGSPAIDIIDRLLAENDRQNIDIPLDGFKDLVLWAYRQ
                     PFVGILDRLIIRGVDFYRERDPGDYPLIEAAKNGYKDIVHRLIDLSVDINLRDRKGRT
                     ALFCAVMQGEKEIVELLINNGADPNIQDNEGVTPLMKVVPTDNSEMVEFLLNAGADTS
                     IKDKRNRLAIDLAKHFPYHMKLLKDATEIQNANKSSSSSNQSANALYTLWYSNRGDLR
                     EEAQFKEKNTPHPF"
     gene            2326..3405
                     /locus_tag="ACF2SP_001311"
     CDS             2326..3405
                     /locus_tag="ACF2SP_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359104.1"
                     /translation="MNALSLIRQMAEKPELNEFIQGIKEGNLTAEHVKLFCQATAQVG
                     VEATRGLWDTNVEQNDFSNDKNREAIYSASLLHGAHNIGHVNNLLHNKNIGISDRSKE
                     AIIGQMEDHSPIGSRRDLAWEKEIATGFLHDAINKFQEGRAKNAAKKGSGDKDFHDGV
                     VDMVGFVMHSLFGVGKAMGRGFLDSQERFSNSAQSNEPTINEHGQLINERLASTPQEP
                     YVQNIGSSKSSIGVSPQLNSTTQNSTIPNEIRKFVTGGLQFKNDDQQVVDNTIEDLKT
                     EVISAFELHNMVQDLPTSKSVQGIEAFKVEQEPSRSNDVTHYFKSILQQLKTTDPQPH
                     KGEIAAHNPGEDNESDISKSIRFRS"
     gene            3704..4609
                     /locus_tag="ACF2SP_001312"
     CDS             3704..4609
                     /locus_tag="ACF2SP_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2359105.1"
                     /translation="MHMSDIKSFLAVVEFHSISLASKRMHISQPTMTRRIQKIEQELG
                     TPLFFSSGIRTELTAKGKELLPYFRQMLDIYQQMYINVGNKTKNPLKLINVGTSVYVA
                     NSIIQDFMHSAEISSSNYFFNIKLLAEPHIIDSLNSGEYDLIIAPPVYDCSSSVVSIP
                     LWKEKILAVVGASHPLARNKKAISITELMSHDVALMEKGFTLRKKLDSLSEGLHTPLK
                     AIFESNSIYNNIALIEKGVGWCLIHERLLNPNLIPVELSDCNMEIEFHCSFLKRREDE
                     RLIWQFLEQIRKWILADYLKQYACP"
     gene            complement(4824..6578)
                     /locus_tag="ACF2SP_001313"
     CDS             complement(4824..6578)
                     /locus_tag="ACF2SP_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2359106.1"
                     /translation="MSFSKVNKILDSNSILKEEIDKPEVLGLAQSSGGEEPPMIPDEM
                     LFALQDRVGVNRVQQACIDGNLHVLERMNCDEFDYVDSYGTTPLMLAIEHDHKAIAYY
                     LLRSRMINLNLHKKDNAGRSALSMAKEKGWDDIVDLIRNTMENPVYNNMELYYATING
                     YLDSVTELLPKIDEPGFATKETWINTYPLFGNIFHAACCGGNLEIVKLLMEYGAEINS
                     RSYNRERHNFYYATTPILVACRYGHAHLVEYLLTQDGVQYDIIELFHIAVEYGHYAVM
                     DLLLRKIRAENIALDLNRGLEFHASWYGTPLDKACRSGQVDIVDRLIAEGIDMKEEDN
                     ISAITTAIWNADNPEYPAIDILDRLLAECDRQGISIPLTAPEIAIIACNRNRADILDR
                     LIIRGFDFNLGNRHGEYPLITAAIKGHEAIVDRLLSLSVDVNQRDSEGKTALFQAARH
                     NNLEIVKLLVNNGADINIPDNKGVTPLMRVAETCFTEVVKYLLNSGADTSLKDKGGRS
                     ALDYASSSSLYFMQENLALIRDADIQKNLYKSPSSSSQSVNNPYMLWSSNRANLPEEE
                     SVKSEIISEEKAFTFQSK"
     gene            complement(6797..11278)
                     /locus_tag="ACF2SP_001314"
     CDS             complement(6797..11278)
                     /locus_tag="ACF2SP_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HFD2359107.1"
                     /translation="MPRYVEGVELTQEGMDAIFTRMGHSNIISGIIYNGEPTIDQDAL
                     DKQGFMPVLAGVGSRSDYGHWLMLIKGSGNQYYLFDPLGKTSGENYQHILADQLPEDS
                     NLSVIPNGPDLNKGLCGYWVASVGLRAHAQLNTDSPPDLVNLGQTITNEMRNELEHDG
                     YRIITDWLRAVADEFPEGDPQPDARALREFTQKALGINIPPPVPPMKDLTPKELPVES
                     NCFQLPYVPVWNGFSLYTDDIVRAAAQYAYDNYLGKPYTGTVESVPANFGGQMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLRKFKDGRTIADVTPEELKKIMIAQAFFV
                     AGRDDEASDAENYRKYHEQSRDAFLKYVKDNEPTLIPDVFKDEEDVNLYAQVIEDKNH
                     DWSSSPAIVLINQAHMVDLVRVKQPPESYLENYFKSMLPWIGPQATEAVFAIQRQFFH
                     ATHEVVAGFDSDNKEPHLVVAGLRRYVIGEDGQPMREAPKEGQREGDLKAFPQAYKLK
                     ETERFMRVDEFLKLPEVQSTFPGAGKHLQGGMPGMNEMDYWNRLNSVNRARCENDVDF
                     CLKQLEIAHHKAKIDPIKVAVQPSEKITRREPNIDEIAAAGIIREILANPDSIQNDHV
                     LINGQKLEEQFFRDLLAKCDMAIVGSLLNDKDISNIDKLMEYEKNTEFHETGEEPVAC
                     RAIGKEWLENYRLDRYNQRRTPEHSIKMALIHMMQDGSWYYRRLNAVAQGRDTGSSFK
                     EVLISALMVPSTFKALSDIQEPEFGKKISQTHPTKIHKGLMSLPPDITQKILNQSEAI
                     IANTTMGLFSDPSAKTYQQMKINQFSHLLARTCSSTTIDEDVGTFFTNGRDNNIRLDI
                     EDPDGLLNAKRVGGIGVGGENEYSVYLPDDVALIPIAVNKGNPNVISLVAVKSPDFIP
                     RHESGYAVEPFLRMQTAKLAEIKSSVENNQVEPIIELQSDLSLVEETDLPSAYKNFIK
                     NTVGPVLENFLNGFMEKDAVALSKALAAFPPDGQWSAFNSEVAGHAKRQMDALKQMVP
                     NIVVLNTLAQCQDALEKQNIAGALEAFKNIPSEKEMGTISRELRGQIQRVRQDLESLQ
                     RAVVTPVVTDEKKVKERYDALIEGTSKKITELEKATLDELDVIKKAISNLGNLRQEVT
                     LLHNEKVRMHSKTDTIDFSDIEKLEQQIQVMDTKLTDAYLLAVTRQISALESTPKNQS
                     ELKAKIATFFDTITEIDMLRNERIKKYGASKDPRDLSDLDKLSGKLQRINQSLVLNLI
                     KTTWSTCALMEYKKFETEETQIKNNITLLETLQESMDKSPKAEKLRGDISKIKDFLVA
                     QQKAYPKITQFIDQLRNLNLTQKKSTTRLPESDSQSPEAGIFRTVGNLIHDVTQYIGY
                     RKLEIRMKKQSLQSLDTYLSTEYLSTEEIGINDLLLRLAYKSPSDLQEGLGISENNAK
                     ELCSLIKRLANKCSAANELEEKLKAIDEISPKLARERESLKFEAIDNDESTGNTMKF"
     gene            complement(11602..12063)
                     /locus_tag="ACF2SP_001315"
     CDS             complement(11602..12063)
                     /locus_tag="ACF2SP_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFD2359108.1"
                     /translation="MSKADLHNCMLFKGVDGKYIDEFLENCEEVELPKGEFLFRQNEI
                     GDSMYIVEQGELQIILEQNSTGSDTKEEQLIGVFKSGALLGELCVFGQLKRAASIRAL
                     ADCRLLKIEGEDFRVRIYSKDLDALLICYNIAKLLSQRFITLLSLRHSPNV"
     gene            complement(12113..13990)
                     /locus_tag="ACF2SP_001316"
     CDS             complement(12113..13990)
                     /locus_tag="ACF2SP_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HFD2359109.1"
                     /translation="MMNESLTEKRNELSLSFAALGVVFGDIGTSPLYAFGQVIKYFPI
                     NDHNIYGILSLIFWSLIIIVSIKYLVIVFRADNDGEGGIIALAGVIRQKIKQPGGWLL
                     FITLVGIGLIIGDGMLTPAISILSAVEGLESLSPNLAKYVLPVTLIILFFLFKMQSIG
                     TGKIGVYFAPVMLVWFITIGILGFLQIIQNPKVLMAINPYYAINFFMIHKYFALFILG
                     GVFLVMTGGEALFADLGHFGKKAIRTGWFAVALPALLLCYFGQGAFVLMHTEDIKYPF
                     FSLSPDWFLPVMIILATLATIIASQAIISAAFSILKQASLLNLIPRLKIVFTSKFEKG
                     EVYLPLINFILALGTCSLVVIFKSSSNLADAYGIAVNLDMLITTVLVGIIAYRCWSWH
                     AFKILIFLLILIIELAFFAGNIPKLLTGGWIPILIAFLGFVVMYTWHCGFEKLRELHH
                     RDALMDAFIIDELNQNKISRQPGMGLYIIDPYDCEGESLLHHLRLNRIFFENMVFVSI
                     KIENKPYIPIEDKFELIKKAEGFYLIFIHYGFTENINLPNELDEMFKRVYLPFDIIKN
                     KLIYFIEIVFVEMTGERQKHMYLWQKHFFSLMIRNAVPDIQFYQLPYNNTIAIGTYYQ
                     F"
     gene            14400..15920
                     /gene="ankQ"
                     /locus_tag="ACF2SP_001317"
     CDS             14400..15920
                     /gene="ankQ"
                     /locus_tag="ACF2SP_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkQ/LegA10"
                     /protein_id="HFD2359110.1"
                     /translation="MATTIPDIETITFTDWFLRIIDTPGVPIDEIEISVIEGFKKTLT
                     DAQFQHLTSLIHLISFIKHRNFSNPTLALRIYIDENTSPLSRTALVEAIRMLPPENNK
                     TYELVCRLAQKNKLDQYTDITKVPPLHIYQGDGVLERYDDWILLFELFDLTQITRGDL
                     IPFLADLLSSKNFDATELRALSKFIKTLDKLGIVSQNFMEKISPHLRNRKNIEQYESF
                     FLKLQVNGLLTEKILEAIYPLLHQLDALEHFFCLYRQELLHDNTPSLLSDTVQSFCNM
                     SIPSKVSYDEPVSTNTPLHLAVIEGNLEHLVRTLAFANRNLLIKCSCENTALLLACKL
                     ADKEAARLILAKMQELGCDVNQRDHHGMTALHWANFYHFNELIKELENAGANPKLRAG
                     NGKTCEYFYQHQFTMQDLKLNGKEIIDGEFKLGDSALTDIAFHMDKIALNLKLTTPSE
                     IAELYSGDGMAQIRSSNRFNLFFRLFRLKLINWLDKQHDFESKPIDDISELTLSKK"
     gene            complement(16044..19115)
                     /locus_tag="ACF2SP_001318"
     CDS             complement(16044..19115)
                     /locus_tag="ACF2SP_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359111.1"
                     /translation="MAYTLTLLGTDTTFTPKPDTGYKYGETLSYVSTLVDRQGEQAQL
                     SDDIPKYRNSNITVVDGPDTMGKAVGDRIARGVASILEAISRGETDISIMAHSRGAVE
                     AILVAHELERIQENCKQGKDSADLMNSKCPLTKTAMKDQKSTFDSLNLAGIANNINNV
                     KLSIFNIDPVPGGDFLGAPVGWKDERFFRVPKIVKEYEQCVYENERTRCFKPIVPKCE
                     SSDTKFNLFSLPGHHGTGSGNLNDQQQAVLVGDTTTEHAQQLMIIKLIDFLNRNGVAI
                     KPGQNGDEEQVNNTSFNALIKPLFDEDGKIKKEELKEKYLTLYQEIAKNKAAYEHFNT
                     TSYKVLGQEQSIQKLFGRYRDDRIILHQVYEDAFLSDVLPHLPGGHFVNYDHARLTIN
                     QELGLEDGLSLNQVIDKTVSRLGEIMKHSSKLANLNNEDAEANDLSQSVLDDNCAHAI
                     QSKEGFNILVDSLEGLIGEVGNIYFHDKHKSMREGERNALFNSVKGAIDLFKSADLND
                     NELATAVREKIDTTIKEILEGKITRLNDDAEILSHKLGENLSEEVNSAFDKLLRTLRP
                     EDVNNDRINEFDGFKNQLIRLLESKPPQIKESRELLEEKLKSFEKTDPPDEVNEDMTS
                     FFGELLEIIPDYDVNKLMEDANQLYDEFDSFKKSLSDFTQLNDSLDYAQWQTKLEEKQ
                     RQLIDQTARYIQKNNLNLEKDIDPLFKDNKSPLYEQIKLVASNYGLENPDFVKLKRQN
                     SELSCKLDEQVEKIKELQQIKERHDADKIKLEKLNQELQVLRDSGSEKEKEVEQIRQE
                     IAALRTGIQFQDRQQDALEKQIKNLNSEIAWLSTDKQQLTREREFQDNRINDLNELVN
                     RLNTDEEQECTAVVRTLKKLTADHLDYLTAKKGNTQNDTIKEQIEITKNLQGILCNTK
                     EYPLPSDRITAFTNQLNEDNKKLSAQHDPDPAWKRFAKSCLITIGVICSGIIPGILAL
                     AAYNHFKDKSSPQSFTDKYKNELSKIKQQSDISKHQTEDETQNLTTTGLAR"
     gene            19439..19927
                     /locus_tag="ACF2SP_001319"
     CDS             19439..19927
                     /locus_tag="ACF2SP_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFD2359112.1"
                     /translation="MKIRAIFFIIAIFLPFTLFASDATCPLSNGINVLTKKRILNICK
                     NGTVVKTFKVALGYKGVGKKKAGDNKTPIGLYGLAHPRKSNQFKVFIPILYPTKKQLA
                     AGYTGRDVGIHGPTQSPGVFSWLNNLPGSTRGCVAVGKNNYIEYVANWVKANPGAKVL
                     II"
     gene            complement(19996..20250)
                     /locus_tag="ACF2SP_001320"
     CDS             complement(19996..20250)
                     /locus_tag="ACF2SP_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359113.1"
                     /translation="MCNEDGSILKILKEIKAIIKEIDPQEEQSISDALRLIRNVIKDG
                     ARQERPLVAEVLKIFNATGSLNFRQIRNSLEHLPGLNEFS"
     gene            20446..20712
                     /locus_tag="ACF2SP_001321"
     CDS             20446..20712
                     /locus_tag="ACF2SP_001321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359114.1"
                     /translation="MPLSVSDCQSNFPVMKNRHHLLYQKQTGIDKSKITQTAAQAANN
                     TNPLKVIIESEYDVDSEKKVTKVGEVKRFFILGLIQKDYFIISI"
     gene            20737..21123
                     /locus_tag="ACF2SP_001322"
     CDS             20737..21123
                     /locus_tag="ACF2SP_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359115.1"
                     /translation="MDIVKAQQDMKVKVNVLRIPANEREANIVAVYSILINKDLMGDM
                     DHIPNVIWQIKSIIENINLDDDDIARSICLIKEKIENSNENYTNKNIMDFLNAFSKKS
                     DLTFRQIRQELAQSNSEMKKILDAYD"
     gene            complement(21375..>22429)
                     /locus_tag="ACF2SP_001323"
     CDS             complement(21375..>22429)
                     /locus_tag="ACF2SP_001323"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFD2359116.1"
                     /translation="LNYWFLGTALLIYFCFLSIISWMGYGLLNKNFKLRYENLDLINK
                     LYAINANLEESELRFRSAFNSAAIGMAIVSLEGMWLKVNQSLCQIVGYSEEELLETNF
                     QSITYPEDLELDLGYVRQLLEGDIRFYHMEKRYIHKNGSIIWILLSASLIRDPENKPL
                     YFISQIQNIDAQKRAEQELQHIAYHDILTGLGNRKLLELSFDQALAHAKRHQTQIAIM
                     FMDLDYFKNVNDKLGHDIGDLLLVEIGKRLQTILRSTDLIVRHGGDEFIIALTELSNV
                     NQVIEIANKILIAISKPITIKRYGISITGSIGISIYPYDGHDSDTLIRKADEALYRVK
                     IGGKNNFQLFNTALHK"
BASE COUNT         7026 a         4347 c         3900 g         7156 t
ORIGIN      
        1 gcataaaaaa tttaaatgtc tggttaatgt tttattaata tacagactgt aacttaaggc
       61 tgattgacct tgttggagaa aatgaatgtc atttagcaaa atcagtaaga ctcttgaaaa
      121 tagtgcgaca ctcatagatg aaaaagctga acaggatgca tctgatttat cgcatacttt
      181 gggatcagac agccaattat taatttcaga ggaaagctca gatgttctgt tggaaaatac
      241 aggagaaaac ttggttcaac aggcttgtat agatggaaat ttagaaaaat taaaagaact
      301 tcttagaggt gatatgtggg gcatggatga agtttcactc aatcaagtga ataacagagg
      361 agaaacgctc ttaatgctag ccattaaata tgggcataag caaatggctc tatttctggc
      421 acgttctctc tttcatttgc ataaaataga taattcaggt aaatcagcgc tgtctctagc
      481 acatgaaaaa ggctgggaag atatagttca acttattttg gaaagtataa ataatcgtga
      541 agctagaatc agggagttgc actatgctgc aatacatggg tacgtagaaa gtgtctctca
      601 attgttaggc atcattgata agtatggatt taaaccacac gggtttatat tagttggcga
      661 tgaaaccccc agatttgaaa acatctttca tgctgcttgt tgcggtggta gtttagagat
      721 agtcaaatta ctgatagagc acggcgctga tatcaattcg aaaagccaat tttcagaaaa
      781 taattatagc cattatcctg ccacacctat acttgtagct tgccggtacg gccatgctaa
      841 tattgtcgag tatctattgg ctcaagatgg aattgagtat gacattaacg aattattgca
      901 tacggcagtt gggtttggtc actttgcggt attggattta ttgcttcgtc atagtaaaga
      961 aaatagcacc cggaatctga atatagttgg ggaatatcct ttgacccatt ttagtgcgac
     1021 ccttttaaca agagcttgtt attcaggaca ggtagatata gtcgatcgct tgattgcaga
     1081 agatattgat atgagtaatg ctgacaacag acaagcattt tggacggcaa ttttttgtgc
     1141 aaaaactcaa ggctcacctg caatagatat aattgaccga ttgcttgcag aaaatgatcg
     1201 tcagaacata gacatacctc ttgatgggtt caaagatctt gtactttggg cgtacagaca
     1261 accgtttgta ggaatccttg accgcctgat tatccgtggt gttgattttt atcgagaacg
     1321 cgatccagga gattatccat tgattgaagc tgccaaaaat ggatacaagg acatagttca
     1381 ccgacttatc gatttgtctg tggatataaa tctgagagat aggaagggta gaacagcact
     1441 tttttgcgca gtgatgcagg gagaaaaaga aattgttgag ctattaatca ataatggtgc
     1501 ggatcccaat atacaagata atgaaggcgt gactccttta atgaaagttg ttccaactga
     1561 taacagcgag atggttgaat ttttattaaa tgccggtgca gacacaagca ttaaagacaa
     1621 gaggaatcgc ttagctattg atcttgccaa gcatttccca tatcacatga agttattgaa
     1681 ggatgccaca gaaatacaaa acgctaacaa atcgtcaagt tcgtcaaacc aatccgccaa
     1741 tgcgctgtac acgctatggt attcaaatcg gggggatttg agagaagaag cacaattcaa
     1801 ggaaaaaaat accccacatc ccttttaaag gaaacagttg ccccctgctg ccctttatct
     1861 gaccaaaata tcagataagg gctcatcata tcgcaagagt gcccgttgta gatttcaacc
     1921 aatagcacta gcatggataa tcaatcattt aaatcatttt gaaatcaagt caaggaagac
     1981 aaaagtcttt gcaaacgagc attttagcgc cacagataac aataccatat ctactaaagg
     2041 ataaattcag cacatggaaa ccttacagtt atcatcaatc gattatcttc cttttggata
     2101 gagcgtaaaa tataagaact aagttaactc tttacccaag cacgagaata cctaacgtct
     2161 tatacctagt acaagataaa aaattacact gtaataattt tccttcaaca ctttgctatc
     2221 atgattacca aattgcgtat aagccattaa aaatacgaat gcgctcataa tgatacaata
     2281 atgctcattt tgtaaaataa aagaacacac agtggaggat acaaaatgaa cgcattatca
     2341 cttattaggc aaatggctga aaaaccagag ctaaatgaat ttatacaagg tataaaagag
     2401 ggcaatttaa cagccgagca tgtaaaatta ttttgtcaag ccacagctca agtaggagtg
     2461 gaggctacaa gaggactttg ggacacaaac gtagagcaaa atgattttag taatgataaa
     2521 aatcgagagg caatttattc agcgagtcta ttacacggtg cgcataatat cggccatgtt
     2581 aataaccttc tccacaataa aaacatcgga attagtgata gatcaaaaga agccataatt
     2641 ggacagatgg aagatcactc accaattgga agcaggagag atctcgcatg ggaaaaggaa
     2701 attgctacgg gttttttaca tgatgccata aacaaatttc aggagggaag agctaaaaat
     2761 gctgctaaaa aaggatctgg agataaagac tttcatgatg gtgttgtaga tatggtaggg
     2821 tttgtcatgc attcactttt tggtgttgga aaggctatgg ggagaggttt tttagatagt
     2881 caggagcgct tttccaactc agcccagagt aatgaaccta ctataaatga acatggacaa
     2941 ttaattaacg aaagattagc tagcacacct caagaacctt atgttcaaaa catcggttcc
     3001 agtaaaagct ctattggggt tagtcctcag ttaaattcaa cgactcaaaa ttctactatt
     3061 ccaaatgaga taagaaaatt tgttacggga gggcttcagt ttaaaaatga tgatcagcaa
     3121 gttgtagaca atacaattga agatttgaaa accgaagtaa ttagcgcatt tgaattacat
     3181 aatatggtgc aagatctacc tacttctaaa tctgtgcaag gaatagaagc ttttaaagtt
     3241 gaacaagaac cctcaagaag caatgatgtt acccattatt ttaagtcaat attacaacaa
     3301 cttaagacaa cagatccaca accacacaag ggtgaaattg ctgcccataa tcctggtgag
     3361 gacaatgaga gtgacatatc taaatctatt agatttcgaa gttagcccaa actcaatctg
     3421 taagttactt tttcaagaag taacttacag atcagcttaa ccccttcgac tttttcagct
     3481 aaaccaatcg cttacatcaa cagttataat gtattacagc ttctcagatg ctcttcggga
     3541 aaagagcaaa ataatcatcc tgaaactcga aataaaattt actaatttta ggtggtcata
     3601 tcaaaatcga acatggttta ttgatatttt tatgatatag cacaatactt atgttaaaca
     3661 attaatgcga ccacactctc aatggtatga ggtaaacata aatatgcata tgtcagatat
     3721 caagtctttc cttgcggttg ttgaattcca ttccatatca ttagcgtcta agagaatgca
     3781 catatcccaa cctacgatga caagaaggat tcaaaaaatt gaacaggaat tgggaacgcc
     3841 cttgtttttt tcttccggga taaggactga acttacagca aaaggaaagg agcttttacc
     3901 ttactttcgt cagatgcttg atatttatca acaaatgtat attaatgtcg gcaataaaac
     3961 gaaaaaccca ttaaagctta ttaatgtagg aacttctgtc tatgttgcta actccattat
     4021 tcaagatttc atgcacagcg cagaaatatc ttcctccaac tattttttta atattaaatt
     4081 actagcagaa cctcatataa tagatagctt aaacagtgga gaatatgact taattatagc
     4141 cccaccagtg tatgattgtt cttcatctgt tgtatctatc cccttatgga aagaaaaaat
     4201 tttagctgtg gttggagcaa gccatcctct ggcgagaaat aaaaaagcca tttcaatcac
     4261 cgagctaatg tctcatgatg ttgcgttgat ggaaaaagga tttaccctgc gtaaaaaatt
     4321 agattctttg tcggaggggc ttcatactcc tctaaaagct atttttgagt caaacagcat
     4381 ttataataac atagcattga ttgaaaaagg ggttgggtgg tgtctaattc atgaacgctt
     4441 gctcaatcca aatttaattc ctgtggagtt atcagattgc aacatggaaa ttgaatttca
     4501 ttgctcgttt ttaaaaagga gagaggatga acgtttgatt tggcaatttc tagagcagat
     4561 tcgaaaatgg attttggcag attatttaaa acaatatgca tgcccataaa cattatgtgt
     4621 agtagtttca cactggatct gagagttacc ggtttttcaa gaagggtctg tcaggtcaaa
     4681 tttagtttat ctaaaatccc catcaaaaat tttttgcctt gacctaaagg tttttcgtaa
     4741 atcctgtaac cgttaaaatt tctttgagac agcttcccat gaccgatttt tatatggtca
     4801 tgggatagaa tctcaagata aagttattta ctttgaaaag tgaatgcttt ctcttctgag
     4861 attatctcag atttaacact ttcttcctct ggcaaattag ctcgatttga cgaccacagc
     4921 atgtatggat tattgactga ctgggatgaa gagcttggtg acttatagag atttttctga
     4981 atatcagcat ccctaatcaa agccaggttc tcttgcataa aatacaaaga ggaagaacta
     5041 gcgtaatcca gtgctgagcg ccccccctta tcttttaagc ttgtatccgc accagagttt
     5101 aataaatatt taaccacctc tgtgaaacaa gtctcagcga cacgcatcaa aggagtcaca
     5161 cctttattat cgggtatatt gatgtccgcg ccattattca ccaaaagttt aacaatttcg
     5221 agattattgt gacgagcagc ctggaataga gctgtcttac cctcactatc cctttgatta
     5281 acatcaacag acaaactaag caagcggtca acaatagcct catgtccctt tatagctgct
     5341 gtaattaaag gatactctcc atgtctattg ccaagattaa aatcaaagcc acgaataatc
     5401 aagcgatcaa gtatatctgc acgattccta ttgcaagcga taatagcaat ctcgggggct
     5461 gtaaggggta tgctgattcc ctgacgatcg cattcggcaa gcaagcgatc cagaatatct
     5521 attgcaggat attcaggatt atctgcattc caaatagcag ttgtaattgc tgatatatta
     5581 tcttcttcct tcatatcgat accttcagca atgaggcgat ccactatgtc tacttgtccc
     5641 gatctacaag ctttatccaa aggagtccca taccaggatg catgaaattc caaaccccgg
     5701 tttaaatcca gtgctatatt ttctgctcta attttacgaa gcaataaatc cattaccgca
     5761 taatgaccat actcaactgc aatatgaaat aactcgatga tatcgtattg gactccatcc
     5821 tgcgttaata aatactcaac aagatgggca tgaccatagc ggcatgccac taaaattggt
     5881 gttgtagcat aatagaagtt atgtcgctcg cgattataac ttctggagtt aatttcagcg
     5941 ccgtactcca tcaataattt gacaatctct aaattaccac cgcaacaagc cgcgtggaaa
     6001 atatttccga aaagaggata cgtatttatc caagtttctt ttgtggcaaa tccaggctca
     6061 tcgattttag gcaataactc agtcacactg tccaaatatc catttatggt agcatagtag
     6121 agttccatat tattatacac aggattttcc atagtgttac gaataaggtc aactatatca
     6181 tcccaacctt tttcttttgc catagaaagg gcagacctac ctgcattgtc tttcttgtgt
     6241 aaattaagat taatcattct acttcgtaac aaataatatg cgattgcttt atgatcatgt
     6301 tcaatggcta gcatcagagg tgtggtgcca taactgtcaa cataatcaaa ctcatcacag
     6361 ttcatcctct ccaatacgtg taagtttcca tctatacatg cttgctgcac acggttaaca
     6421 cctactctat cctgcaaagc aaatagcatt tcatctggta tcattggtgg ttcttctcca
     6481 cctgaacttt gggctaaacc aaggacttcc ggtttatcta tttcttcttt aagaattgaa
     6541 ttgctgtcta gtattttgtt gactttgcta aatgacatcc tattttctcc atcaaaatta
     6601 atggacttca gtttacaatt tgattattaa ttaaacatta ccaatgtatt tcaattttct
     6661 ataccttatg caatctttat tgaacagaat agagctgtgc tctcagggat actatttata
     6721 tgaagttata tgaagtaatc tgggccttgt cggcccaatt tcagaggtta aaactgttct
     6781 ggcttaaaaa atccaattag aatttcattg tatttccagt agactcatca ttgtcaatcg
     6841 cttcaaattt caaactttcg cgctctctgg ctagttttgg ggagatctca tcaatagctt
     6901 tcaatttctc ttccaattca tttgctgcag aacatttatt agccaaacgc tttataagtg
     6961 aacacaactc cttggcattg ttttcggata tccctaaccc ttcttgcagg tcagatggac
     7021 ttttatatgc caacctaagg agaagatcat tgataccaat ttcctcggtt gacaaatact
     7081 cggttgacaa atatgtatcc aagctctgaa gcgattgctt cttcattcta atttcgagtt
     7141 tcctataacc gatatactgg gtgacgtcat gaattaaatt tcctactgtt ctgaatatcc
     7201 cagcttctgg ggactgacta tccgattctg gcaatctcgt tgtactcttc ttttgggtta
     7261 gatttaaatt gcgtaattgg tcgatgaatt gtgttatttt aggataggct ttttgttgtg
     7321 caaccagaaa atcctttatt ttagaaatat ccccccttag tttttctgct tttggtgatt
     7381 tatccattga ttcttgaagt gtttcgagca aagtaatatt attttttatt tgtgtttctt
     7441 ctgtttcaaa ttttttatat tccattagag cgcaagtgct ccatgtagtt tttatcagat
     7501 tcaaaaccag ggattgatta atacgctgta acttgccgct taatttatcc aaatcagaca
     7561 gatcccgcgg atctttcgat gcaccgtatt tcttgattcg ctcattacgc aacatatcaa
     7621 tctcggtaat tgtatcaaaa aatgtagcta tttttgcttt caattcagac tgatttttgg
     7681 gtgtgctttc aagagctgaa atttgcctgg ttactgcaag taaataagca tcggttaatt
     7741 tggtatccat gacctgtatt tgttgttcca gtttttcaat atctgaaaaa tcaattgtat
     7801 cagttttact gtgcatccgt actttttcat tatgcaacag cgtcacttct tgtctgaggt
     7861 tacctaaatt agaaattgcc ttcttaatga catcaagttc gtcaagtgtt gctttttcaa
     7921 gttcagtaat tttcttcgat gtgccttcga taagggcatc atagcgttct ttgactttct
     7981 tttcatcggt tactactgga gtaacaacag cacgttgcaa tgattccagg tcttgcctta
     8041 cgcgttggat ttgtcctctt agctctctgc taatggtacc catctctttt tcagatggga
     8101 tgtttttgaa tgcctccagt gcgccagcga tattttgttt ttccagcgcg tcctggcatt
     8161 gagccaaggt gttgagcact actatatttg gaaccatttg tttaagagca tccatctgtc
     8221 ttttggcatg ccctgctact tcagaattga aggctgacca ttgtccgtcg ggtggaaatg
     8281 ccgctaaggc tttacttaaa gcgaccgcat ccttctccat gaatccatta agaaaatttt
     8341 ccaaaacagg gccgactgta ttttttataa aatttttata tgcagagggt aaatctgtct
     8401 cctccactag gcttaaatcg ctttgtaatt cgataattgg ctctacctgg ttattttcaa
     8461 cagagctttt gatttcggct aacttggcag tttgcattct taaaaacggt tcaaccgcat
     8521 agccgctttc atgacgtgga ataaagtcag ggcttttaac agcaaccaag ctaatcacgt
     8581 taggattgcc tttattcact gctataggta tcaaggcaac gtcatcaggc aaatagactg
     8641 agtattcatt ctcaccacct acgccaattc cacctactcg ttttgcgttt agcaaaccat
     8701 ccggatcttc aatgtctaat cttatgttgt tatcccttcc attggtaaaa aatgttccga
     8761 catcttcatc gatagtggtg ctggaacatg ttcttgccag taaatgagag aattgattga
     8821 ttttcatttg ctggtaagtt tttgctgaag gatcagaaaa taatcccatg gtagtattcg
     8881 cgataattgc ttcagattga tttaatattt tctgagtgat gtctggtggc aaactcatca
     8941 gacctttatg aatttttgtt gggtgtgttt gtgatatttt tttaccaaat tcaggctctt
     9001 gaatatctga caaagcttta aaggtagagg gtaccatcaa agctgaaatc aaaacctctt
     9061 tgaatgaaga gccagtgtct cgcccttgag cgaccgcatt aagcctcctg tagtaccagg
     9121 aaccatcttg catcatatgt atgagagcca ttttgataga atgctcaggg gttcttcttt
     9181 gattatacct atcaaggcga tagttttcta accattcttt accaatggcg cgacacgcaa
     9241 cgggttcttc tcctgtctcg tgaaactctg tattcttttc atactccatc agtttatcga
     9301 tatttgatat atccttatca ttcagtaatg atcctactat tgccatgtca catttagcca
     9361 acaaatcgcg gaaaaattgt tcttccagtt tttggccatt aatgagcaca tgatcattct
     9421 gaatggagtc aggattggcg agtatttctc taataatgcc agccgcagct atctcatcga
     9481 tattaggttc tctcctcgtt attttttcgc tcggttgaac agcaaccttt ataggatcaa
     9541 ttttggcttt gtgatgggcg atttctaatt gttttaaaca aaaatctaca tcgttttcac
     9601 accttgccct gtttacagag ttcagtcgat tccaataatc catttcattc atacctggca
     9661 ttccaccttg taaatgtttg ccagcaccag ggaaagtact ttgtacttca ggaagtttta
     9721 aaaactcgtc cactcgcata aaacgttccg tttctttcaa tttataagct tgaggaaaag
     9781 cttttaaatc accttctctt tgtccttctt taggcgcttc tctcataggt tggccatctt
     9841 caccaatgac ataacgacgt aaaccagcaa caaccaaatg aggctctttg ttgtcggagt
     9901 caaaacccgc caccacttca tgagtagcat gaaaaaattg tctttgtatg gcaaaaacag
     9961 cttcagtggc ctgggggcct atccaaggca gcatggactt gaaataattt tccaaataag
    10021 actcaggggg ttgtttcacc cggaccaaat cgaccatgtg tgcctggtta atcaaaacaa
    10081 tggccggact tgaactccaa tcatgatttt tatcttcaat cacttgggca tacaaattga
    10141 catcctcctc atctttgaag acatcaggaa ttagtgtggg ctcattgtct ttcacatact
    10201 ttaagaaagc atcacggctt tgttcatgat actttctata gttctcggca tcggatgctt
    10261 catcatcacg cccagctaca aaaaatgcct gggcaatcat gatttttttc agttcttcag
    10321 gggtaacgtc agcaatagta cgaccgtctt tgaattttct taatgtttcc cctcgtaatt
    10381 tggctttacg tgcttcttca acaatcaatt ctgcataagc catggtccgt aaagtatggg
    10441 aaagcccatg gtgttgtcta tacaccattt gcccaccaaa atttgccggt actgattcaa
    10501 ctgtcccagt ataaggtttc cccagataat tgtcataggc gtattgtgcc gcagctcgaa
    10561 cgatgtcatc ggtatacaat gaaaacccat tccaaactgg cacgtatggc agttgaaagc
    10621 aattagattc aacagggagc tctttgggag ttagatcttt catcggagga acaggagggg
    10681 gtatattgat accaagagct ttttgtgtaa actctcttaa tgctctcgca tcaggttggg
    10741 gatcaccttc cggaaattca tctgctacag ctcttagcca gtcagtaatt attctataac
    10801 cgtcatgttc cagttcattg cgcatttcat tggtgatggt ttgccccaga ttcactaaat
    10861 cagggggact atctgtgttt aattgagcat gcgctcttaa accaactgaa gcaacccaat
    10921 agccgcataa tcccttgttt aaatccgggc catttggtat gacagagaga ttagagtctt
    10981 caggtagttg atcagctaaa atgtgttgat aattctcacc ggaagtttta cccaatgggt
    11041 caaagaggta atattggtta ccagatcctt ttattaacat gagccagtga ccgtaatcac
    11101 ttctggagcc tacccctgct aacaccggca taaaaccttg tttgtcgaga gcatcttgat
    11161 caatagtcgg ctccccatta tatataatac cgctaataat attgctatgc cccatacggg
    11221 tgaaaatagc atccatacct tcttgagtta attctacccc ttcgacatat ctaggcatga
    11281 tggtttctcc caaaataata ttttaaaatg taccaatcag cacattcacc tatgctttta
    11341 ttctactgtt caggtattaa ccaaatatta cttgagtatt actattccta atgccttatg
    11401 cattaatgtc atattgagaa tgacaacatc atacttttgc accggttcca taagaggcaa
    11461 gctgaggctg ccgggttgtc ttggaagtta ttggaaatta aataactaca gaagggtaag
    11521 aataaaaaaa tcagttggtt tcttaagcgt taaaactacg agaacacaac tctgggatta
    11581 gcaatcccct agctgctgaa atcacacatt gggagaatga cgtaaactta aaagagtaat
    11641 aaaacgctgg cttaatagtt ttgcgatatt ataacagatc aataaagcat ccaaatcttt
    11701 tgaatagata cgaaccctga aatcctctcc ctcaattttt aataatctac aatcagccag
    11761 ggcacggatt gatgcagctc gttttagctg gccaaataca cataattcgc caagcaatgc
    11821 cccgctttta aatactccaa tcaattgttc ctctttagta tcagagcctg tcgaattttg
    11881 ctctagtata atttgtaact cgccttgttc gactatatac atcgaatcac caatttcatt
    11941 ttggcgaaaa agaaactcac cttttgggag ttccacttct tcacaatttt ctaaaaattc
    12001 atcgatatac tttccgtcta cacccttgaa taacatgcag ttatgtaaat cagctttgct
    12061 catttcactg tccttaaact ctaggaaatc caataatctc ctttcgattg acttaaaatt
    12121 gataataagt ccctattgca atggtattat tatacggtaa ctgataaaac tgtatatctg
    12181 gcacagcatt acgtatcatg agagaaaaaa agtgcttttg ccatagatac atgtgttttt
    12241 gcctttctcc agtcatttca acaaaaacga tctcgataaa atagattaat ttattcttaa
    12301 ttatatcaaa aggtaagtat actcttttga acatttcgtc taattcatta ggcaaattaa
    12361 tattttcagt aaacccataa tggataaaaa ttaaataaaa cccttccgct ttctttataa
    12421 gctcaaattt gtcttcaata gggatataag gtttattttc aattttgata cttacaaaaa
    12481 ccatattctc aaaaaaaata cggttaagac gaagatgatg aagaagactt tccccttcgc
    12541 aatcataagg gtcgataata tataacccca tcccgggttg tcttgaaatt ttgttttgat
    12601 ttaactcatc aataataaag gcatccatca gagcatctct atgatgcaat tcccgtagtt
    12661 tctcaaagcc acagtgccag gtatacatca ccacaaatcc aagaaacgcg attaaaatag
    12721 ggatccagcc tccggttaac aacttgggta tattgcctgc aaaaaatgca agttcaataa
    12781 ttaatatcaa aagaaaaatc agtattttga acgcatgcca actccaacaa cgataagcaa
    12841 tgatcccaac taaaacagtg gtgataagca tatctaaatt aacggcaatg ccgtatgcat
    12901 cagcaagatt tgatgacgat ttaaaaatca caacaagcga acatgtacct aaagccaaaa
    12961 taaaattgat aagtggcaaa tagacttcac ccttctcaaa tttggacgtg aagacaatct
    13021 taaggcgtgg aatcagatta agcagcgatg cctgttttaa aattgaaaat gccgctgaaa
    13081 taattgcctg tgatgcaata atcgttgcga gcgtagccaa aataatcatc acagggagaa
    13141 accagtctgg cgataaacta aaaaatggat atttgatatc ttctgtatgc atcaatacaa
    13201 acgctccctg accaaaatag cataaaagca atgcaggaag cgccacggca aaccagcctg
    13261 ttcgaatggc ctttttgcca aaatgcccta aatcagcgaa taaggcttca ccaccagtca
    13321 ttactaaaaa taccccaccc aatatgaaga gagcaaagta tttatgtatc ataaaaaaat
    13381 taatcgcata ataaggattt attgccatca gtactttggg attctgtata atttgtaaaa
    13441 aacccaaaat tcctatggta ataaaccaaa caagcataac aggagcgaaa taaacaccaa
    13501 ttttccctgt acctatgctc tgcatcttaa agaggaaaaa taaaataatt aatgtcactg
    13561 gtagaacata tttggccaaa ttgggagaca aagattccag accttcgaca gcacttaata
    13621 tagatattgc tggagttaac attccatcac caatgataag cccaatacca accaaagtaa
    13681 tgaataaaag ccagcctcct ggctgtttta ttttctgcct tatcacccct gctaaagcta
    13741 taatgccgcc ttcgccatca ttatccgcac gaaaaacgat caccagatac ttaatactga
    13801 ctataatgat cagcgaccaa aaaatgagcg ataaaatacc ataaatatta tggtcattga
    13861 ttggaaaata ctttattacc tggccaaatg cataaagagg gcttgtccct atatcaccaa
    13921 aaacaacccc caaggctgcg aatgataagc tgagctcatt cctcttttct gttaaagact
    13981 cattcatcat ccgggactac tcccaatatc ataataaatc ctttattaat tattattttg
    14041 agtatagtac tatctgatat caaaaatgaa ttgacattta gtatctgaag gtcgatgaat
    14101 tacagatttg cctgcatatc cgctctctat gactcaattc cttaaaatga gcgattaaca
    14161 acttcaaaca gcttcggtta taaatgcaaa ccatatgaaa ttggtatgcg ttataagctt
    14221 taatatggcc atattttgca tagttcaaaa agaagaaaat aactttctaa aattttttaa
    14281 tactaaatgc tctctgtaca tgacaaaaag acctgtcatg tatagaacta aatgctattt
    14341 ttttgttata aaaatgaatc tattggttat aataacatca tttggtttgg gaaatgatta
    14401 tggccacaac aattccagat attgaaacaa tcacttttac cgactggttc ctgagaataa
    14461 ttgacactcc cggtgttccc atcgatgaaa ttgaaattag tgtaatcgag ggtttcaaga
    14521 aaacattgac agatgctcaa tttcaacact taacctcatt aatccattta attagtttca
    14581 ttaagcacag gaatttttcc aatcctaccc tggcactccg aatatatatt gatgaaaata
    14641 ccagcccttt atctagaact gccttggttg aagcaatcag gatgcttccg cctgaaaata
    14701 ataaaaccta tgagttagta tgccgtttgg ctcagaaaaa taaactggat cagtatactg
    14761 acataacgaa agtgcctcct ctgcatattt accaggggga tggcgtgctt gagcgatacg
    14821 atgactggat tctgcttttt gaactgtttg atctaaccca aataacccgt ggtgatttaa
    14881 ttccatttct tgctgattta ttaagcagca agaattttga cgccactgaa cttagagctc
    14941 tttccaaatt tattaaaacc ctggacaaat taggtatagt aagccaaaat tttatggaaa
    15001 aaatctctcc tcatttgcgt aacagaaaaa atatagagca atacgagtca ttcttcctta
    15061 aattgcaagt caatggcttg ttaaccgaaa aaattctgga agcaatttac ccgctacttc
    15121 atcaattaga tgcccttgaa catttttttt gtctctatcg tcaagagcta ttgcatgata
    15181 acaccccttc attactatcg gataccgtac aatccttttg taatatgagc atcccaagta
    15241 aagtaagcta tgatgaaccg gtatcaacaa acaccccatt acatctcgct gttattgagg
    15301 gcaacctgga gcatctcgta cgaaccttag cctttgccaa tcgcaattta ctaataaaat
    15361 gctcgtgtga aaataccgct cttttgttag cttgcaagct tgcagataaa gaagcggctc
    15421 gattgatttt ggcaaaaatg caagaattag gctgtgatgt caatcagaga gatcatcatg
    15481 gtatgaccgc cttgcactgg gctaattttt atcattttaa cgaactaatc aaggaactgg
    15541 aaaatgctgg ggcaaaccca aaactacgag caggtaacgg gaaaacttgc gaatattttt
    15601 atcagcatca attcactatg caagatttaa agttaaatgg caaagaaatc attgatggtg
    15661 aattcaagtt aggtgatagt gcgctaacag atattgcttt ccatatggat aaaatagcct
    15721 taaatttaaa attaactacc ccatccgaga ttgcagagct gtattcagga gatggaatgg
    15781 cacaaattcg ttcgagcaat cgattcaatt tatttttcag gttatttagg ctcaaattga
    15841 ttaactggtt agacaaacag catgacttcg aatctaaacc aattgacgat atatcggaac
    15901 tcaccctttc aaaaaaataa cattggctaa actccattta ctttaaatgc aatgtattta
    15961 aatatgcctc aggggtaact gaggcataca gactttatga atatttgagt aatgctctct
    16021 cttaaacaaa aatttcttaa tcttcaccta gccaatccag ttgttgtcaa attctgggtt
    16081 tcatcttccg tttgatgttt ggagatatca gattgttgtt taattttgct taattcattc
    16141 ttgtatttat ccgtaaaaga ttgtggggat gatttatctt taaagtgatt atacgcagca
    16201 agggcaagaa tacctggtat aatcccacta caaattactc caatggtaat cagacagctt
    16261 ttcgcaaatc gtttccaggc tggatctgga tcatgctgag cgcttaattt tttattatct
    16321 tcattaagtt gattggtaaa cgctgtaatt cgatcactcg gtaatggata ttctttcgtg
    16381 ttacataaaa tcccttgcaa attttttgta atttcaattt gttcttttat agtatcattt
    16441 tgtgtatttc ctttttttgc agttaaataa tcgagatgat ctgcggtcaa tttcttcaac
    16501 gtacgaacaa cagcagtaca ctcctgttct tcatctgtat ttaatctatt cactagttca
    16561 tttaaatcat tgattcgatt gtcttgaaat tcacgctctc tagttaactg ctgtttatca
    16621 gtgcttaacc aagcaatttc actgttaaga ttcttaattt gcttttctaa agcatcttgt
    16681 tgtctatctt gaaattgaat tccagttctt aaagccgcaa tttcttgtct tatttgttca
    16741 acttcttttt ctttctctga gcctgaatcc ctcaaaacct ggagttcttg gtttaatttc
    16801 tctaatttta ttttatcagc atcatgtctt tcttttattt gttgcaactc ttttattttt
    16861 tcaacttgtt catctaattt acatgacaac tcactattct gccgctttaa cttcacgaaa
    16921 tctggatttt ccaaaccata attgctggca acaagtttga tttgttcata cagtggagac
    16981 ttgttatctt taaaaagagg atcaatgtcc ttttcaaggt tgagattgtt cttttgtata
    17041 tagcgagcag tttgatcaat caattgtctt tgtttttcct ccaatttggt ttgccattga
    17101 gcatagtcta atgaatcatt aagttgtgta aaatcagaga gtgacttttt aaagctgtca
    17161 aattcatcat acagttgatt ggcatcctcc attaatttgt ttacatcata atctggaata
    17221 atctctaata actctccaaa aaagcttgtc atatcttcat tcacttcatc tggaggatct
    17281 gttttttcga atgactttaa tttctcctct aaaagctccc tggactcttt aatttgaggt
    17341 ggtttcgatt ctaatagtct aattaattga tttttaaaac cgtcaaactc attgattcga
    17401 tcattgttta catcttccgg ccttagtgtt cgcagtaatt tatcaaacgc agagttaact
    17461 tcttcagata aattttcgcc cagtttgtgg gaaagaattt cagcatcatc attcagtcta
    17521 gttattttac cctccaaaat ttcttttatg gtcgtatcta ttttttccct aacagccgtt
    17581 gccagttcat tatcgttcag atcagcactt ttgaataagt caattgctcc ttttactgaa
    17641 ttaaataatg catttctttc tccctctctc atcgatttat gcttatcatg gaaataaata
    17701 ttaccaactt caccgataag tccttctaag ctatcgacta aaatattaaa tccttctttg
    17761 ctttgaatcg catgtgcgca gttatcatca agaactgatt gcgaaagatc gttagcctct
    17821 gcatcctcat tgtttaaatt cgccaattta gatgaatgct tcattatttc acctaatcta
    17881 gatactgttt tatcaatgac ttgattgagt gacagtccat cttctaaccc aagctcctga
    17941 ttaatagtaa gacgggcatg atcataattt acaaaatgac cgccaggaag gtggggcaaa
    18001 acatcactta agaatgcatc ttcatagact tggtggagaa taatacgatc atctcggtac
    18061 ctaccaaaca atttttgaat tgattgttcc tggccaagaa ccttgtagga agttgtatta
    18121 aaatgctcgt aggcagcttt gtttttggct atctcctgat atagagtcaa atatttttct
    18181 ttaagctctt ctttcttgat cttgccatct tcatcaaaca atggtttgat cagagcgtta
    18241 aatgaagtat tattcacttg ttcttcatca ccgttttgtc ccggctttat agctactcca
    18301 ttgcgattca gaaaatctat cagttttata atcattaatt gttgtgcgtg ttctgtggtt
    18361 gtatcgccca caagaacagc ttgttgttga tcgtttagat tgcctgagcc agttccatgg
    18421 tgtccaggca gactaaaaag attaaatttt gtatcgcttg attcacattt tggaacaata
    18481 ggcttaaagc agcgtgttct ctcattttca taaacgcatt gctcatattc cttaactatc
    18541 ttgggtactc taaaaaagcg ctcatctttc cacccaactg gtgcgcccag gaaatcaccg
    18601 cctggaaccg gatcaatatt gaatattgat agttttacat tattaatgtt attcgcaata
    18661 cccgcaagat tcagagagtc aaaggtactc ttctgatctt tcatcgccgt tttggttagc
    18721 ggacacttac tattcatcaa atcggcagaa tctttaccct gcttacaatt ttcctgaatt
    18781 cgttccagtt catgagccac caaaatcgct tctactgcac ctcgactatg agccataata
    18841 ctaatgtctg tttcacctcg gctaattgcc tctaatatgc tagctacacc acgtgcgatc
    18901 cggtcaccaa ctgctttccc catagtgtct gggccatcaa ctacagtgat atttgaattt
    18961 ctatatttag gtatgtcgtc agataattgc gcttgctcac cctgcctatc aaccagagta
    19021 gatacgtaac tcaatgtttc tccatactta tatccagtgt caggttttgg agtaaaagtg
    19081 gtatctgtac ccaataaagt aagtgtgtat gccatgatgt cagctcctca gtgaacataa
    19141 tgacagttta tattttatac attaaggtca aattactttc acatagaata aaagcatggg
    19201 gcattggaaa aacaaataat agccaatagg gttagatggg aaatcagact tatttccgag
    19261 gaatatgtta aaaattataa gctcatccac aaatccttat catttaccaa gaaaactgag
    19321 gatctcaggg ttcctcgcgc tatgattttt tctgaaagat ttagcctgcc agctctatga
    19381 ataaaagcaa tattgtggga taattattgc ccaacttcaa caatattagc ctatttatat
    19441 gaaaataaga gcaatttttt tcataatcgc tatttttctt ccatttactt tatttgccag
    19501 tgatgcgact tgtcctttat caaatggaat caatgtactc actaaaaagc gaattttaaa
    19561 tatttgtaaa aacggcaccg tcgttaaaac ctttaaagta gcactcgggt acaaaggcgt
    19621 tggtaaaaaa aaggcagggg ataacaaaac tccaattggt ttatatgggt tagcccatcc
    19681 aaggaaatcc aatcaattta aagtctttat tccaattctc tatccgacta aaaagcaatt
    19741 agccgcagga tatactggta gagatgtcgg gatccatgga ccaacccagt cgcccggcgt
    19801 gtttagttgg ttaaacaact tacccggctc aactcgtgga tgtgttgctg ttggcaaaaa
    19861 caactacatc gagtatgtgg caaattgggt aaaagccaat cctggagcta aggttttaat
    19921 tatttaaatt gagacgaaat ttgataccgc ctatttattt tatgtagaat gccctgatag
    19981 tggtcgtctt gatgattaag aaaattcatt taaaccaggt aagtgctcta acgaattgcg
    20041 gatttgtcta aaattaaggc tacccgtagc attaaatatt tttagaactt cagcaactaa
    20101 aggtctctct tgtcgggccc catcttttat cacattgcga atcaggcgaa gtgcatcact
    20161 aattgattgc tcttcttgtg ggtctatttc ttttataatc gctttgattt cttttaaaat
    20221 tttgagaatt gagccgtctt cattacacat taaaggacac tctagcaagt gtagagtgcg
    20281 agtccatctg aactcctcac atcaaataga aggtttaatc taataaggcc ggtctttatg
    20341 gctgtgtttt acttaaccca gatatcaatt tttttggtaa taaaaactct tggtatttgt
    20401 tctatacttt tatttaaata taaatacact tagtttggaa ttcatatgcc cttgtctgta
    20461 tcagactgtc aatcgaattt cccggtgatg aaaaacaggc accatttact ttaccagaag
    20521 caaactggga tcgataaatc caaaataaca caaactgctg ctcaagcagc taataataca
    20581 aaccctctaa aagtaattat tgaatctgaa tacgatgtcg atagtgaaaa gaaagtaact
    20641 aaagtcggag aagtaaaacg attttttata ctcggattaa ttcaaaagga ttattttata
    20701 atatcaattt gatttttata acctgagagg attgaagtgg acatagtaaa agcacagcaa
    20761 gacatgaagg taaaagtaaa tgttttaaga attccagcca atgaaagaga agctaacata
    20821 gtagctgtct attcgatatt aattaacaaa gacctgatgg gagatatgga tcatatccca
    20881 aatgtcattt ggcaaataaa atcaattatt gaaaatatca atctggatga tgatgatatt
    20941 gcaagatcaa tttgtctaat taaagaaaag atagaaaatt ccaatgaaaa ttataccaat
    21001 aaaaatatca tggattttct aaatgcattt agtaaaaaat cagatttgac tttcagacaa
    21061 atccgacagg aattagctca atccaattct gaaatgaaaa aaattctgga tgcctatgat
    21121 tgagatgatg tgattcatag acatcatttc aattcattcg tattatccat cgtctaaaat
    21181 aagcaaaaaa gcataggcta atttttacct tgatactaaa aaatagaggc tctgtttgcc
    21241 atcgatatgg catctctcgt cagacaaata accaagaaaa tggcaatgtt gatatttaga
    21301 gaggaaacag ggtaataatc tgacaaatta agaacaaagg ggctggaaag aagcttgttg
    21361 attaaaaaat tattttactt atgtagtgcg gtattgaaca attgaaaatt attttttccc
    21421 cctattttca ccctatataa tgcttcatca gcttttctaa ttaatgtatc tgaatcatgg
    21481 ccgtcatatg gatagatact tataccaata ctgccggtaa ttgaaatacc ataacgtttg
    21541 attgttatag gcttgcttat agctattaga attttatttg ctatttctat aacttgattt
    21601 acattggata attcagtaag cgcgatgatg aattcatcgc caccatgcct gacgattaga
    21661 tcagtagacc ttaaaatcgt ctgtaatctt tttccaatct caactaataa tagatcccct
    21721 atatcatgac ccaatttgtc attcacattt ttaaaataat ccaaatccat aaacataata
    21781 gcaatttgcg tttggtgacg tttcgcatgt gctaatgcct ggtcaaatga taactctaat
    21841 aactttctat ttcccaaacc agttaaaatg tcatgatatg cgatgtgttg aagttcttgc
    21901 tctgctcgct tttgggcatc aatgttttga atttgcgaga taaaatagag tggtttattc
    21961 tcgggatctc ggattaaaga agcgctaagt aaaatccaaa tgatactacc atttttatga
    22021 atatagcgtt tttccatatg atagaatctt atatccccct ctaataattg cctcacatag
    22081 cccaaatcga gctctaaatc ctctggatat gtgatcgatt gaaaattagt ttccaataat
    22141 tcttcttctg agtatcctac aatctgacaa agcgattgat taacctttaa ccacatcccc
    22201 tccaaagaaa ctatagccat accaatagca gcagaattaa atgcggaacg gaaacgtaac
    22261 tcactttcct ccaaattagc gttaattgca tacaatttat taattaaatc caaattctca
    22321 taacgtaatt taaaattctt attaagaagt ccatatccca tccaggatat aattgataaa
    22381 aaacaaaagt aaatcagcaa tgcagttcca aggaaccaat agttaagtg
//
LOCUS       DAXLDT010000045        22237 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXLDT010000045 DAXLDT010000000
VERSION     DAXLDT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22237
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.045"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            179..1162
                     /locus_tag="ACF2SP_001324"
     CDS             179..1162
                     /locus_tag="ACF2SP_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HFD2359117.1"
                     /translation="MKNNNKSVITVDLNDPVKSLEYLEEAFQIGTFILKNSPVNFKRL
                     AKVLSDLDTFYNLDFKTKIEISGQISGGGATRGYFPLGVESGLVDVFEKKESYSFGWP
                     DNEIPDSDTPNLSLVAPNIYPDHGYINQDDVGGLIKDFANTAKELSKLIEIILFNGKT
                     ALPIDESSDFQSLLRSFRYYKKTELDPPNTGANSLHTDWNLITLVYADADGYQYRDNE
                     GNFQNIIPETDDCLIVNFGDFLSAWTGGKIVSPWHQVVEAKKKDVRNSLVFFYYPAAS
                     SSAVKNPIYNQGEPLSLFVDQSDPLNRLWDFSKIETMNEMYAEKWKQVSRK"
     gene            complement(1287..1736)
                     /locus_tag="ACF2SP_001325"
     CDS             complement(1287..1736)
                     /locus_tag="ACF2SP_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFD2359118.1"
                     /translation="MSLLLTSPAFEHNDFIPDEFTCHGLDCPPPLAWENAPPNTKSFV
                     IIMDDPDASGGLWVHWVLFNIPASYNELDAAIDLPDSAISVKNSWGKTGYGGPCPPRG
                     THRYFFRLYALDTFLSLDEKATKKDVEYAMQGHILESSELICKYTKL"
     gene            1954..2319
                     /locus_tag="ACF2SP_001326"
     CDS             1954..2319
                     /locus_tag="ACF2SP_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359119.1"
                     /translation="MKDLLVYVLFFIFSSLINVAWADNNLCIAINNFGTVPINVSGAN
                     SLEDHIVKPRETLILPEKEVAASCFPDWGCDVIIIPIEQNAKGLYLKQLPKGSRIIYS
                     QPDLYYVNRTANVPCIPDL"
     gene            2358..3209
                     /locus_tag="ACF2SP_001327"
     CDS             2358..3209
                     /locus_tag="ACF2SP_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2359120.1"
                     /translation="MTSYEEKKVKIPGFTIALKIWNPNNPNPVLCLHGKMDNAASFDL
                     LAPLLSDRQLVAVDYPGTGLSSHYPEGVVPHWKNDAFLMCHVIKALGWKRFDIIAHSL
                     GSFLATVITIAQPKQVNKLVFLDILGPTVHIIENSMAYLPLNIESYLISGKQPRTVFS
                     SLDAAIKDRMNIGNISYQAAQALVQRGTKQDNEGWVWTFDPRLRCISATIPHEDEIRA
                     MFRAIDVPVCLILANQGVSYPESVFKGRSQSIKNLTIHRVQGGHHVHMDDPAPVAEII
                     SQYLSSK"
     gene            3336..3854
                     /locus_tag="ACF2SP_001328"
     CDS             3336..3854
                     /locus_tag="ACF2SP_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1453 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359121.1"
                     /translation="MPRWFRRIVDEGERLLVEVTDELRDESEVHKAASQLHLRIEQVL
                     NANRARLEKPVIEAAEGFLHDLGVILEEDSMLRFPHAPVNQYLLAEKCTDIIYNYKPS
                     LESAPGIWNQIKAHINNFIEIIFGLENVLPTTQTMFAKDVSFTRCKRNLSRLKESLES
                     DAPDLLSVLVNC"
     gene            complement(3976..5361)
                     /locus_tag="ACF2SP_001329"
     CDS             complement(3976..5361)
                     /locus_tag="ACF2SP_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359122.1"
                     /translation="MKKNIIMFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKL
                     VLISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARII
                     QGLGGSLTVPVGRLIILRTCKRHELITKMSIVVMVASLGMLLGPLLGGIITDYFSWRW
                     IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKT
                     DIVYSASITLLSALLLIGYTKHSRNKKNPIIKVTLLNIRTFRIAVLGNLLTRLSFGGI
                     PFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRFGYKKLLLLNTVLVC
                     FSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMST
                     VQQLSQSFGVAISALLVSLFTSQVSQHFVLTVKIFHLTFFALGMLTILSGLIFTSLKK
                     EDGKELIESPT"
     gene            complement(5390..6646)
                     /locus_tag="ACF2SP_001330"
     CDS             complement(5390..6646)
                     /locus_tag="ACF2SP_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HFD2359123.1"
                     /translation="MLKNITRALSLSLLISHNICANEASSIETISQPTTIQFLEKFGA
                     GFANLEHKNLGDRVQLYLPGHNSKTGIVKLKLPNGSKLSYGEIVMYGGDMFGNPAKPI
                     SNCSEKNRLACFQAQFDALGTLGNKQDKRCSNPLNQITHLDRFFANLAEEIEKSKQNG
                     TNDWDYYKQHDSAISKELNKLTCGGSIISGFIPFGNYIKLAEVNYDHFVPDSLVAYKT
                     GHRFALETALKAYEKKQQGLIDEAHKLLELAYAQNAFANHYLTDSFSAGHMRTPRRAI
                     GHDILLPSVLNLLIANLMHNEDNQHGLNVVNHEGISWLAYGDGYLYKPEAELQRVIIL
                     DAMQRSADSIHSTFMNGVIPEHYPEMDVFPDYSKIEQINDTAPLFKVENGVLLKRVNN
                     KDHYDHHWTKYWSGLVTLIEFQVNKI"
     gene            6767..8101
                     /gene="rlmD"
                     /locus_tag="ACF2SP_001331"
     CDS             6767..8101
                     /gene="rlmD"
                     /locus_tag="ACF2SP_001331"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFD2359124.1"
                     /translation="MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGE
                     VVEFQYTRVKKDFDEGKLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQS
                     HLLDLLSRYGHTEPQSVLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
                     TEINQCPILNSKIDTDIVHLRKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQ
                     DELKIREFAQQFQYKVFLQPGGLDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQ
                     VNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVER
                     AYMNAKSNHITNVDFYAANLDDVMEVRSLVNTSFSKVLIDPPRSGALEIVKQIDSIDP
                     ERIVYVSCNPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG"
     gene            8107..10311
                     /gene="relA"
                     /locus_tag="ACF2SP_001332"
     CDS             8107..10311
                     /gene="relA"
                     /locus_tag="ACF2SP_001332"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFD2359125.1"
                     /translation="MVRVKDTTPLMPDGSIDVEMWLHHLGSKGYLDNLELVRAACTLS
                     QLAGQDHATETGQTCLQQGLSMADLLADLEVDQETLAAAIIFENVHYADLSIDDVEEQ
                     LGHNIAKLVKGIEKMSAMNNFQALNKYPQNKNQIDNIRKMLLAMVDDVRVVLIKLAEC
                     LCILRTAGHLSETVRKQLATEAMEIYAPLANRLGIGAIKWEMEDLAFRHLHPEEYKAI
                     AKGLKAKRLERDSFVNRIVEQLEHQIRATGARHFAVYGRSKHIHSIYKKMKRKNVSLD
                     EIYDATAVRILVDTEAQCYEVLGMVHTLWKQIPAEFDDYIINPKSNGYQSLHTAVEGP
                     EGRVFEVQIRTFHMHDLAEMGVAAHWKYKEGAVSQKESHERKIEWLRDVLAWHQEMAA
                     NKGVAENVTTEFLEDRVYVFTPDGDVLDLPHGVTPLDFAYHVHSNLGHRCRGAKVNGH
                     IVPLTYQLKTGDRVEVLTGKEIKPSRDWINPHLNYLKTARAKAKVLHWFKMQDYEDNV
                     RDGRELLEKELKSLGIKSDKLNEVITALNYKKADDLYASLGRGDIKIGQIVNRLAPPD
                     TSEQNLQKFVRQQQKPEVTGSDLRIEGVGNLLTFMARCCQPVPGDEIVGYITIGRGVS
                     VHRKDCPNIIHASEKQKRRFIQVNWGTATRENYVVDVLIKAFDRSELLKDVTSLLSNE
                     KAHVYALQTHSNKHENMAYITLTVEIDGLNSLSRLLTKLEQIPNVLEARRQL"
     gene            complement(10352..10717)
                     /locus_tag="ACF2SP_001333"
     CDS             complement(10352..10717)
                     /locus_tag="ACF2SP_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFD2359126.1"
                     /translation="MNINLETAEQHAVQAYSDKKIQINSIIYESSLIVSKKEIITDFV
                     IKDIQDINDSYIDLLLRSKPELIIIGHLQTGKTLPVTLISQLSQKRIGIECMSIGAAC
                     RTYNVLLSEHRAVVAGFIF"
     gene            10776..11969
                     /gene="alaC"
                     /locus_tag="ACF2SP_001334"
     CDS             10776..11969
                     /gene="alaC"
                     /locus_tag="ACF2SP_001334"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFD2359127.1"
                     /translation="MSQFPRIKRLPPYVFNTINQLKMEARARGEDIIDFGMGNPDQPT
                     PPHIVNKLIEVAQRPDTHRYSMSKGIPRLRRAMATWYLRHYDVHLDSETQILATIGSK
                     EGLAHLALAISGPGDTVLVPDPAYPIHTYGFIIAGANVKQIPLIDEVQFLAAVEDAIN
                     RTWPKPKALVLNFPANPSTHCVEYEFFEKVVDLAKRNNIWIIHDLAYADIVFDGYKAP
                     SILQVPGAIDIAIETYSMSKSYNMPGWRVGFACGNEELVAALTRIKSYLDYGTFTPIQ
                     VAAITALEGPDDCVLEIRNLYEKRRNLLCDGLNDIGWEVSKPKGTMFVWAPIPLNYRA
                     MGSLEFSKYLLKEAQVAVSPGIGFGQQGDGYVRFGLIENKDRIRQALRNLKALFKRDG
                     LLKAI"
     gene            11974..12783
                     /locus_tag="ACF2SP_001335"
     CDS             11974..12783
                     /locus_tag="ACF2SP_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359128.1"
                     /translation="MNVIINTESDCDSKEGKLLKSQGLAVLNLLACGGYDLVNPPIGN
                     LLKSSHSLEGDWVVLTPMHWQASHNDAVIVALDKDLRVTDEEVKYWFDLYSAYLAEEG
                     ASLYFYDKYTWLLRVDDKPPLNAKPIYQVLNKSLMPELSQLDETMYWQKFFTESQMFF
                     SSNARKSLINGVWAWGSGKLKTKKTTSICTDKHFLAVAQAYSSNVTLYDPSVNVSGFE
                     VVLLHDIDSLSELHQVEIKKIPAHWYWNNCALIKAKSHWFTRLWRSLTHAD"
     gene            12773..14512
                     /gene="recJ"
                     /locus_tag="ACF2SP_001336"
     CDS             12773..14512
                     /gene="recJ"
                     /locus_tag="ACF2SP_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFD2359129.1"
                     /translation="MLIKQRQQPEFTVNLPNVPDVLKRIYVTRGISDESQLDKQLQTL
                     LPFHTLKGINEACLRLENALRSEQRILIIGDFDADGATSTALAITALRAMGARFVEYL
                     VPNRFEFGYGLTPAIVEVASKWQPHLIITVDNGIASFEGVDAANKMGIDVLITDHHLP
                     AESLPDACAIVNPNQPGCLFPSKSIAGVGVIFYVMLALRRHLSNCNWFNEINMPEPNM
                     ASFLDLVALGTVADVVGLDQNNRIMVNQGMMRIRQGQCREGIKALIEIAGRDYSRLRE
                     SDLGFAIAPRLNAAGRLDDMSLGIECLITTDPKMARNFCQQLDELNLERRQIESEMKE
                     QAMLALEKLSINDEENKHLPVALCLHDKTWHQGVIGILAGRMKEKYHRPVIAFASVSE
                     HELKGSARSVPDLNIRDVLAAVDKDNPGLILKFGGHAMAAGLSISPELLNDFRSAFVS
                     EVLKHLDLSQCEGEILTDGPLQSHELSLETAQLIQQAGPWGQAFAEPVFDNVFEILDQ
                     RIVGKNHLKMTLISSQGGEPLDAIAFNVDLKSWPNHRTKYIHVAYKLDINFYQGRTRL
                     QLLIQAMNPVQQG"
     gene            14591..15583
                     /gene="dusA"
                     /locus_tag="ACF2SP_001337"
     CDS             14591..15583
                     /gene="dusA"
                     /locus_tag="ACF2SP_001337"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFD2359130.1"
                     /translation="MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGA
                     VQNNPGRALYFNSVEHPIAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQA
                     GRFGACLMREPEHVADCIRAIKEVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
                     DKLIVHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTIEEINSH
                     LMYVDGVMLGRLACDNPYQIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSPANLSLS
                     ILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLNLEAINQVEFV"
     gene            15684..18374
                     /gene="secA"
                     /locus_tag="ACF2SP_001338"
     CDS             15684..18374
                     /gene="secA"
                     /locus_tag="ACF2SP_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFD2359131.1"
                     /translation="MLSTLIKKMFGSRNERTLRRMEKSVMAINAFEPKMQALSNEELA
                     GKTQEFKERFNNGESLDELLAEAFATVREVSLRTLGLRHFDVQLIGGMVLHEGNIAEM
                     RTGEGKTLVATLPAYLNAISGRGVHIVTVNDYLAKRDSQWMRPIYEFLGLTVGVIYPD
                     MSHKEKQEAYKADIVYGTNNEYGFDYLRDNMAFSLTDKVQRELNFAIVDEVDSILIDE
                     ARTPLIISGAAEDSSELYIKINSLIPQLKKQEEEGDEGDYTVDEKQKQAHLTDAGHLH
                     IEELLTKAKLLDPGESLYHASNIMLMHHVNAALKAHAMFHRDIDYIVKDNQVVIVDEH
                     TGRTMPGRRWSEGLHQAVEAKEGVPIQNENQTLASITFQNFFRMYNKLSGMTGTADTE
                     AYEFQQIYNLEVVVIPTNRSMIRKDEADLVYLTQADKFQAIIEDVRECGVRRQPVLVG
                     TVSIEASEFLSQLLKKENIKHQVLNAKFHEKEAQIIAEAGRPGAVTIATNMAGRGTDI
                     VLGGSLAADLANLPADASEQEKEAVKKEWQKRHDEVIAAGGLRIIGSERHESRRIDNQ
                     LRGRAGRQGDPGSSRFYLSLEDNLMRIFASERVASMMRRLGMQPGEPIEHSLVTRAIE
                     NAQRKLEGHHFDVRKQLLDYDNVANDQRQVIYTQRSSIMAMTDTQEVVEMMREEVMDS
                     LVDTYIPPQSLEDQWDPQALSDVLSDEFKIKAPVPDWIDKDHSIQPEQIKEKILALAI
                     EHYDEKVRKVGRPVISQFEKSIILQTLDNHWREHLAAMDQLRQGIHLRGYAQKDPKQE
                     YKKEAFSLFTMMLDNLKYEVIRILSSVEIQTEEDAQVVEEQRRADQIRKMNLMHESLS
                     ENDEASETQTFRRQEKKIGRNDPCPCGSGKKYKACHGSLV"
     gene            18371..18775
                     /gene="mutT"
                     /locus_tag="ACF2SP_001339"
     CDS             18371..18775
                     /gene="mutT"
                     /locus_tag="ACF2SP_001339"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFD2359132.1"
                     /translation="MKVAVAIIIDEKQRILITQRPFHVAHGGFWEFPGGKLEPHESPE
                     DALVREIREELGIIVNEYRFLGYVDYDYPDKHIQLIIFMVTRFTGNPLCQEGQLNMKW
                     VKKEELNINDFPKANHAVFDLINIPELQYQAL"
     gene            19004..19822
                     /locus_tag="ACF2SP_001340"
     CDS             19004..19822
                     /locus_tag="ACF2SP_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFD2359133.1"
                     /translation="MITKQNGIYTLTLSLLLFSLQINAATDYQTYETVCGEPTDMLSF
                     LERGGIATNPCVVPPRSVLITSGYQYQQLIGEGVQHNFPAAAIQLGLPGYFEVDLLLP
                     NYINQTVDPRIGFSQTQLIVNHVLWFNNKWVITASGTFVFPSGSASFGSPNPGGGVIG
                     ILSYNFNSQLNLTSNLGITTQSEPSYDGGQSYTSVNPDLILSWTKNKISLFAEIYGQS
                     KTAPDEGSGFSTDAGVLYQVKKNIVIDFEVDQRITGLLNGVERYYGGGITIQFN"
     gene            complement(19867..20613)
                     /gene="zapD"
                     /locus_tag="ACF2SP_001341"
     CDS             complement(19867..20613)
                     /gene="zapD"
                     /locus_tag="ACF2SP_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFD2359134.1"
                     /translation="MHDHMITFQLATHFLSRIALRLEYLFKTINEACNESHEVIHRFA
                     LKNIIEIVEIVEKPELKSRFIKELIRIEHVLKKANLLNHSELFDDLETQIHVLNHVPG
                     RFSNKIHDDEFLKTLRQIHHPNTKECEFNSPHLVLWFDSDPLMRQKTISQWVSCLKDL
                     EDTVKIYLSLLRGVTQYTPIRANNGFYQHSLSPKSLNHLILLRIDKKLKMIPKIQLGH
                     HSLTIRLYELVTGQEIRDKTIEMEIAFCQI"
     gene            complement(20851..21456)
                     /gene="coaE"
                     /locus_tag="ACF2SP_001342"
     CDS             complement(20851..21456)
                     /gene="coaE"
                     /locus_tag="ACF2SP_001342"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215598.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFD2359135.1"
                     /translation="MVYSVGLTGNIASGKSTVAEFFSELGINVIYADKIAKELTSKNT
                     PCYQDIISHFGSSVVLNNGELDRKRIRDIIFSNSNERLWLESLLHPVIREKIEEQLIA
                     CTSPYCLIEIPLLFNKHHYPYLQKVLLVIAPLESQLDRIVKRDHCTKKQALAILATQP
                     NLEQRLEAADDVLINESGLSELKAKVNKLHQKYLREAKIKQ"
     gene            complement(21428..21901)
                     /locus_tag="ACF2SP_001343"
     CDS             complement(21428..21901)
                     /locus_tag="ACF2SP_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359136.1"
                     /translation="MRNYIFYGLLLLIGLSHHGFTAPMMKKGSYWKCVTYDKANKAWT
                     AQNSYRKVAINVAFAACKKESQLPATCKTSISNCEGFINGVSTRPMWRCTAIDITAQP
                     WESNFYSNRDDAALAAQAYCKENSTLPATCYINMVTCANKNEGAHSDGLFSGTNW"
     gene            complement(21898..>22237)
                     /locus_tag="ACF2SP_001344"
     CDS             complement(21898..>22237)
                     /locus_tag="ACF2SP_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2359137.1"
                     /translation="QELRQAGFSLAVDDYGTGHASISYLQHFPFNYLKIDKLFVQAIG
                     SNDIIESLNDAIIQMAKGLNLVTIAEGVETKDQANYLANNGVRLQQGWYYSKGLSIAE
                     LTALLKGEKI"
BASE COUNT         6994 a         3778 c         4571 g         6894 t
ORIGIN      
        1 caaaatcgag gaccggtttt caatttttct caaatcaaga taagaagcaa acactagagg
       61 aaaaatctgt cttcactcct taaggtagta ttgtaaatta aagtatggtt gatattgata
      121 gttacattag atagttattc atggtttaaa aagttcttaa caggaaataa tgagaaagat
      181 gaaaaacaat aataagtctg taattactgt tgatttaaat gatccggtga agagtctgga
      241 atatcttgaa gaggccttcc agattggtac ttttattcta aaaaattcgc cggtaaattt
      301 caaaagatta gcgaaggttt tatctgatct ggacacattt tacaatttgg attttaaaac
      361 caaaatcgaa atttccggac aaatttccgg cggtggtgca accagaggct attttccact
      421 gggtgtagaa agtggtcttg ttgatgtatt tgaaaaaaag gaatcttact cgtttgggtg
      481 gcccgataat gaaattccag attcagatac cccaaattta tcattagttg ccccaaatat
      541 ctatcctgat catggttata ttaatcaaga tgatgtcggg ggattgataa aagactttgc
      601 caataccgca aaagaattgt ccaagttaat tgaaatcatc cttttcaatg gaaaaactgc
      661 tttaccaatc gatgaatctt cagattttca atcgttgtta cgaagcttca ggtattataa
      721 aaagactgag cttgaccctc ctaacacagg agcaaattcg cttcacacag attggaacct
      781 gattacgttg gtatatgcag atgctgatgg gtaccaatat agagataatg agggcaattt
      841 tcaaaacata atcccagaaa cagatgattg ccttatagtt aattttggag attttttatc
      901 ggcttggact ggtggaaaaa ttgttagtcc ttggcatcag gtagttgagg caaagaaaaa
      961 agatgtaaga aactctctgg tcttcttcta ttatccagcg gcctctagct cagctgttaa
     1021 aaatcctata tataatcaag gtgagcctct tagtttgttc gttgatcagt ctgatccttt
     1081 aaataggtta tgggactttt ctaaaataga aaccatgaat gaaatgtatg cagaaaaatg
     1141 gaaacaagtg agtagaaaat aggattcagt ttaagtcatt aagcaatgat tatcataaaa
     1201 cgtatttttt gctggtacaa ttttttctaa aaaaatatgg atatgctgtt attagtgtta
     1261 ttgttctctg ctgatctcaa catgtattag agtttagtgt atttacatat gagctcactt
     1321 gactcaagaa tatgaccttg catagcatat tcaacatctt tctttgttgc tttttcatcc
     1381 agagataaaa aagtatccaa agcatataac ctaaaaaaat atcgatgtgt tcctctaggg
     1441 gggcatggtc cgccgtaacc tgttttgccc caactgttct taacgcttat cgcggaatcg
     1501 ggtaaatcta tagcagcatc cagttcatta tagcttgccg gtatgttgaa taaaacccaa
     1561 tgaacccata atcctcctga cgcatctgga tcatccataa ttatgacaaa ggatttggta
     1621 tttggcggag cgttttccca agcaagcggt gggggacaat ccaatccatg gcaagtaaat
     1681 tcatcaggaa taaaatcgtt atgctcaaat gccgggcttg ttagtaataa actcaaaata
     1741 ctctccttat ctctttcgga tacagcattg tgaatcttaa ctttttgtta tttttcccta
     1801 ctacccttaa attatattag catggtaatt ttggatctgc cattcatcaa agcaataatg
     1861 aaattgaaaa tattgtgctc ttcattgact cttactcctc ctacttatta gtatataaac
     1921 ctcttaaata tttgccccaa ctggagagtt ttaatgaagg atttattggt ctatgtttta
     1981 ttttttattt tttcttcatt aattaatgta gcttgggctg ataataactt atgcattgct
     2041 attaataatt ttggtacagt gccaatcaat gtttctggcg ctaattcttt ggaagatcat
     2101 atagtcaaac ctagagaaac attaatttta cctgaaaaag aggttgctgc ttcttgtttt
     2161 cctgattggg gctgtgatgt cattattatt ccaatcgagc aaaatgctaa aggactatac
     2221 cttaaacagc ttcccaaggg atctcgtatt atttatagtc agccagattt atattatgtg
     2281 aatcgaactg ctaatgttcc atgcatacca gacctctgac tctttgcatt ggcccgtgtt
     2341 aataaaaggt atctatcttg acatcttatg aagaaaaaaa ggttaaaatc ccaggtttta
     2401 ctattgctct taaaatttgg aatcccaata atcctaatcc agtactgtgt ttgcatggca
     2461 aaatggataa cgcagcaagt tttgatttgc tggcgccttt gttatcagac aggcaattag
     2521 ttgcagtaga ttatcctgga acagggttgt caagccatta tcctgaaggt gttgtccctc
     2581 attggaaaaa tgatgctttt cttatgtgcc atgttatcaa ggcattagga tggaagcgct
     2641 ttgatattat tgcccactca ttaggttctt tcctggcaac tgttataact attgcccaac
     2701 ctaaacaagt taataaattg gtatttttag atattttagg accaacggtt catattattg
     2761 agaacagtat ggcttattta cccctaaata ttgaaagcta tttaatatcc ggtaaacaac
     2821 caaggactgt atttagcagt ctggatgctg caatcaagga ccgcatgaat attggtaata
     2881 taagctatca agcagctcaa gccctggttc aaaggggtac aaaacaagat aacgagggct
     2941 gggtttggac atttgatccc cggttacgct gcattagtgc aactatacct catgaagatg
     3001 aaattcgagc catgtttcgt gctatagatg tgccagtttg cctaattctc gctaaccaag
     3061 gtgtatctta tcccgaatct gtttttaaag gacgctccca atcaataaaa aatttgacca
     3121 tccatagggt acaaggggga catcatgtgc atatggatga tccagcccct gttgcagaaa
     3181 ttatttctca atatttaagc tctaaataaa actggtttat taaagaataa caagtataga
     3241 tggactcttg ctctaaatgt tgatgcaggg atgattgata atgaatgatt tcatgatgta
     3301 taattggcca atctatttaa tagtggagca ttaatatgcc tagatggttt agaagaatag
     3361 tagatgaggg agaaaggctg cttgtcgagg taactgatga attgcgtgat gaaagcgaag
     3421 tgcataaagc agcaagtcag ttacatttac gtatcgaaca agttttaaat gccaatcgag
     3481 ctagacttga aaaaccggtt attgaagcag ctgaaggttt tcttcatgat ctaggggtta
     3541 tcctggaaga agattccatg ttgagatttc ctcatgcacc agtaaaccaa tatctactcg
     3601 cagagaaatg taccgatatt atttacaatt acaaaccgtc cttagagtca gcaccaggga
     3661 tttggaatca gatcaaagct catattaata actttattga aataattttc ggtttagaga
     3721 atgttttacc aacgacgcag actatgtttg caaaagatgt aagtttcacc agatgcaaaa
     3781 gaaacctttc cagactaaag gaatcacttg aaagtgatgc gcctgatttg ttgtctgtgt
     3841 tggttaattg ttaagtcgtc attgaattta attaatttac ttacgcatta atttttcttg
     3901 aatgaccaga tactgcatcg atatgcattg tcttggtcat cctgctgtct gcttagtgat
     3961 gcaactgatg cgaatctagg tgggtgactc aatcagttct tttccatcct ctttttttaa
     4021 gctggtaaat attaatcctg aaagaatcgt aagcatccct aaagcaaaaa aggttagatg
     4081 aaaaatcttg actgttaaaa caaaatgttg cgaaacctga gaagtgaata aactgaccag
     4141 tagggcagaa attgctactc caaaactttg ggataattgt tgaactgtgc tcataatgct
     4201 tgtcgcataa ctcatatcat tatcgtcaat attcgcatac gcaagggaat tcatccctgt
     4261 gtattgcaga gcaatgaaaa atccataaag aaatgttaaa aaaccaatgt agatgacaga
     4321 agtgctttga tttattgtgg caaaggacca gagagaaaaa cagactaaaa cggtatttaa
     4381 aaggagtaat tttttataac cgaatcttcg taagatggag aaagataatg gtttaaccag
     4441 gaatacgcct aatgctactg gtgtaagtaa cattcctgat aactggggag aatattctaa
     4501 aataatttgg aagagtaaag gtaataaaaa gggaattcca ccaaaactta aacgagttaa
     4561 taaatttcca agtacggcaa tacgaaatgt tcttatattt aacaaagtaa cttttattat
     4621 aggatttttt ttgttacgag aatgtttggt atatccaatt aatagcaagg cggataaaag
     4681 agtaattgaa gcggagtaga caatatccgt tttcgtctca ctaaacaaag acaaacctaa
     4741 agtcaagaat gcaaggcctg ctccgaacat aataaatcct gatttatcca aaggcggaac
     4801 cgatctgggc aggatagttg gaagcagttt gtgagataaa agcatggcga ttatgccaac
     4861 agggatatta acccaaaata tccaccgcca tgagaaataa tcagtaatga taccgcccag
     4921 taaggggcca agtagcatac ctagcgatgc aaccatgacg acaatactca tttttgttat
     4981 taattcgtga cgcttacaag ttctcagtat aattaaacgt ccaacaggca cggtcagtga
     5041 gccgcctaaa ccctgtataa ttctggctaa taccaattcc caaagacttt gagtgaatcc
     5101 gcaccataag gaacttagag taaagacacc aatagctgct ataaatactt tttttacacc
     5161 atatttatca gcaatccatc cacttatggg aataaagatt gccaaactta aaagatagct
     5221 aatgagcacg agtttgagat caattggatt gacttctaaa ctatgtgcca tcaccgggat
     5281 tgccgtgttg agaatagttg tatcaatagc ctccataaac atggcgcatg atacaatgaa
     5341 cataataata tttttcttca taatgcctgt tttatggaag ttagaacgtt taaattttat
     5401 taacctgaaa ctctattaaa gtcacaagcc cgctccagta ttttgtccaa tgatgatcat
     5461 agtgatcctt attattaact cgtttaagca aaacgccgtt ttcaacttta aataatggag
     5521 ctgtatcatt tatctgttca atttttgagt aatcgggaaa cacatccatc tctgggtagt
     5581 gttcagggat aactccattc ataaaagtac tatggatact gtctgcagaa cgttgcatgg
     5641 cgtctaagat gatcactctt tgtaattcgg cttctggttt gtataaatag ccatctccat
     5701 aggcaagcca acttattcct tcatgattca ctacatttaa cccatgttga ttatcttcat
     5761 tatgcatcaa attggcgata agaagattta aaacagaagg cagtaatata tcatgtccta
     5821 tggctcgtct tggagtgcgc atgtgtcctg cagaaaaaga atcagttaag taatgatttg
     5881 caaaggcgtt ttgagcatag gctaattcaa ggagcttgtg agcttcatcg ataagtccct
     5941 gctgcttttt ctcataagct tttaatgctg tttccaaagc aaatctatgg cctgttttat
     6001 aggctacaag tgaatcagga acaaagtgat catagttgac ttcagctaat ttaatataat
     6061 tgccaaaggg tataaaacca ctgattatac ttccaccaca agtcagtttg tttaattcct
     6121 tgctaatagc cgagtcgtgc tgcttataat aatcccaatc gtttgttccg ttttgttttg
     6181 atttttcgat ctcttcagcc aaattggcaa aaaaccgatc aagatgggtt atctgattaa
     6241 gagggttaga gcaacgtttg tcttgcttgt tacctaaagt ccccagagcg tcaaactgcg
     6301 cctggaaaca agctaatctg tttttctcag agcaattgga tatgggtttt gctggatttc
     6361 caaacatatc accaccgtac atcacgatct cgccataact tagtttactc ccattgggca
     6421 atttcagttt tacaatacca gttttgctgt tatggcctgg caaataaagt tgaactctat
     6481 cccccagatt tttatgttcc agattggcaa agcctgcacc aaatttttct aaaaattgaa
     6541 tggtcgttgg ttgagatatt gtctcgatag aagatgcttc attggcgcag atgttatggc
     6601 ttattaacaa tgacaaagac aaagctcgtg tgatattctt caacattatg cattcccttg
     6661 attatttggc cctttatttg ggcatttttt gttttttata tccatacaac attctaaaaa
     6721 tgtagcatga gaattttgta attgataatt atttgagaat catttcatga gaaaagttaa
     6781 gccaaaattg aatttaacct cacaaaccgc tcggatcgta aatttaagcc atgatgggag
     6841 ggggatagca cgagttaatg gcaaagcaac ttttattcag ggagcattac ctggtgaggt
     6901 ggttgaattt cagtatacac gggtaaaaaa ggattttgat gaaggtaaat tattatcaat
     6961 tgttgagccc tctactctca gggttgaacc aaaatgccct cattatcaaa tgtgtggggg
     7021 ctgttctttg caacatatga gtgccgagga acaaattcgt ttcaaacaat cccatctttt
     7081 ggatttacta tcccggtatg gccatactga acctcaatca gtgttgtcgc ctttaacaag
     7141 ccatcattgg aattatagaa ataaagcgcg cttaagtact cgctttgtag aaaagaaaca
     7201 aagcaccatg gttggattta gagagaggaa taatccaagg ttcattacag aaataaatca
     7261 atgtcctatt cttaattcaa aaatagatac tgatattgtc catttgagaa aattaatcga
     7321 tacgatggaa gataagcact gtatcgcaca aattgaagtg gctgcaggtg ataatgaagt
     7381 ggcgctgatt ttccgcaatc tatctccatt aacagagcaa gatgagttga aaatcaggga
     7441 atttgcgcaa caattccaat acaaggtatt cctgcagcca ggtggacttg attcggtttt
     7501 ttgtttttat ccttcagatg ctcatgctta tttgagttat gagctgcctg attatcaaat
     7561 aacttttcaa tttcatccca acgattttac tcaggtaaac gccgagttaa acagaaagat
     7621 ggtaacgcag gcaattcagt taatggaatt aaaaaattct gatatcgtgc tagacttatt
     7681 ttgtggactg ggtaattttt cgcttcccat ggctaaacat tgctctcgag ttatcggtgt
     7741 cgaaggcaat aagaacatgg ttgaaagagc ctatatgaat gccaagtcta atcatattac
     7801 caatgtggat ttttatgccg ctaatcttga tgatgtgatg gaagttagga gtctggttaa
     7861 tacgtctttt agcaaggttt taatcgatcc tccccggtct ggtgcgcttg aaattgtaaa
     7921 acaaattgat tcaattgatc ccgagcgtat agtatatgta tcttgtaacc cgataacatt
     7981 agctcgagat acagatatat tggttaatca aaaaggttac gttttgataa cagctggggt
     8041 aatggatatg tttcctcata cagcgcatgt cgagtctatt gccttgtttc aaaaaggata
     8101 atatgtatgg taagagtaaa agatacgact ccgttgatgc cagatggcag tatcgacgta
     8161 gagatgtggt tacatcatct tggttcaaaa ggatacttgg ataatctcga actcgtaaga
     8221 gctgcctgta ctttaagtca attggctggt caggatcatg ccactgaaac agggcaaact
     8281 tgtttacaac aagggctatc aatggctgat ttacttgctg acctggaagt agatcaggaa
     8341 actctggctg ccgctattat ttttgaaaat gttcattatg cggatttatc tattgacgat
     8401 gttgaagagc aattggggca caatatcgct aaattggtca aaggcataga aaaaatgagt
     8461 gccatgaaca atttccaggc tttaaataaa tacccgcaaa acaaaaatca aattgacaat
     8521 attcgtaaaa tgctccttgc gatggttgat gatgtgcgag ttgttttaat caagctggcg
     8581 gagtgtttat gtattttaag aacagcaggg catctgtctg aaacagtacg caaacagctg
     8641 gcaaccgaag ccatggaaat ttatgcgcct ttagctaacc gcttgggaat aggggcaatc
     8701 aagtgggaaa tggaagattt ggctttccgg catttgcatc cagaggaata caaggcgatt
     8761 gccaagggct tgaaggctaa acgattggaa agggacagct ttgtcaatcg cattgtcgaa
     8821 cagcttgaac atcagattcg agcaacagga gctcgccact ttgccgttta tggtcgctca
     8881 aaacatatcc atagtattta taaaaagatg aagcgcaaaa atgtatcatt agacgagata
     8941 tatgatgcaa cagcggttcg tattctggta gatactgaag cacaatgtta cgaagtgttg
     9001 ggtatggttc atactttgtg gaagcaaatt ccagcagagt ttgatgatta catcatcaat
     9061 ccaaaatcca atggctatca gtctcttcat accgcggtag agggtccgga aggaagagtt
     9121 tttgaagtac aaatcagaac ttttcatatg catgatttag cagaaatggg tgtggcagcc
     9181 cattggaaat ataaagaggg cgcggtatca caaaaagaga gccatgagcg taaaatagaa
     9241 tggttaagag acgttttggc atggcaccag gaaatggcgg ctaataaagg tgtcgcagaa
     9301 aacgttacga ccgagtttct tgaagacaga gtgtatgtat ttactcccga tggcgatgtg
     9361 ttggatttac cccatggggt gactcctttg gattttgctt atcatgtgca tagtaatttg
     9421 ggacatcgtt gccgaggtgc caaggttaat ggtcatattg tcccattgac ttatcaattg
     9481 aaaacaggag acagggttga agtactgact ggaaaagaaa tcaaaccatc aagggattgg
     9541 attaatcctc atctgaatta tttaaagacc gcaagagcca aggctaaagt attgcattgg
     9601 tttaaaatgc aagattatga agataatgtg agagatggtc gtgagttatt ggagaaggaa
     9661 ttaaaatctt taggtattaa atcggataaa ctcaatgaag tgattacggc attgaattat
     9721 aaaaaggcgg atgatcttta tgccagcttg ggacgaggtg atattaaaat aggtcagatt
     9781 gttaatcgat tggcacctcc agatacttca gaacaaaatt tacagaagtt tgtaagacaa
     9841 caacaaaaac cagaggtcac agggagtgat cttcgtattg agggagttgg gaacttattg
     9901 acatttatgg cgagatgctg ccaacctgtt cccggtgatg aaatagtagg atacatcacg
     9961 attggaaggg gggtttccgt tcatcgaaaa gattgcccta atattataca tgccagtgaa
    10021 aagcaaaagc gacggtttat tcaggtgaat tggggtacag caacacggga aaattatgtt
    10081 gttgatgtgc tgattaaggc ctttgatcgt tccgagttat tgaaggatgt tacttcgctg
    10141 ctttcaaatg aaaaagccca tgtttatgct ttacagaccc atagcaataa acatgaaaat
    10201 atggcttata tcactttaac tgttgagatc gatggtttaa acagtttgtc gcgcttatta
    10261 actaagttgg agcaaattcc taacgtgctg gaagcaagaa ggcaattata gctttggtat
    10321 atttagccag agccttctca aaaaattgat attagaagat aaaccctgca acaactgctc
    10381 tgtgttcgct caataagacg ttataagttc tgcaggcggc gcctatggac atgcattcga
    10441 taccaattct tttttgcgat aattgactga ttagagtgac aggcagcgtt ttgcctgttt
    10501 gcagatgacc aataataatt aattcgggtt tgcttcttaa taataaatca atatatgaat
    10561 cattaatgtc ctggatgtct ttaattacaa aatcagtaat gatttctttc ttagaaacaa
    10621 ttaaactact ttcataaatt atggaattaa tctgaatctt tttatcgctg taagcttgca
    10681 ctgcatgttg ctctgcggtt tctagattga tattcatgat tttaaaaagt tcattagttt
    10741 ataatggcta acagtatatc acagaggtca atattatgag tcaatttcca cgcattaaac
    10801 gcttaccccc ttatgtattt aatacaataa accagcttaa gatggaagcg cgggcgcgag
    10861 gagaggacat tatagacttt ggcatgggga atcctgatca accaacccca cctcatattg
    10921 ttaataagct tatagaggtt gctcaaaggc ctgatacgca tcgatattca atgtcgaaag
    10981 gaatcccgag attgcgtcga gcgatggcaa cctggtatct tcgacattat gatgttcatc
    11041 tggacagtga aacacaaata ttagctacaa taggatctaa agagggactt gcacatttgg
    11101 ctttggcaat ttcaggtcca ggtgatacgg tgcttgttcc tgaccctgct tatcctatcc
    11161 atacctacgg atttatcatt gctggtgcta atgtaaagca aattccttta atcgatgaag
    11221 ttcaattttt ggctgcagta gaagatgcta ttaacagaac ctggccaaaa cccaaagcct
    11281 tggttttgaa ttttccggca aatccaagta ctcactgtgt cgaatatgag ttttttgaaa
    11341 aggtggttga tcttgctaag cgtaataaca tttggattat tcatgatttg gcctacgcag
    11401 atatagtttt tgatgggtat aaagcccctt cgattttaca agttcctggt gccatagaca
    11461 ttgcaattga aacttactca atgtccaaat cttataatat gcctggctgg cgtgttgggt
    11521 ttgcttgtgg taatgaagag ttagtagctg ccttaacacg tataaaatcc tacttggatt
    11581 acggtacttt tactcctatc caggttgctg caattacagc actggaaggc cctgatgatt
    11641 gtgtgttgga aataagaaat ctttatgaaa aacgacgcaa tcttttgtgt gatggactga
    11701 atgacattgg ttgggaagtg agcaaaccta aagggaccat gtttgtttgg gcacctattc
    11761 ctctcaatta ccgcgctatg gggtctcttg agttcagtaa atatttattg aaagaagcac
    11821 aagttgctgt ttcacctggt ataggttttg gacagcaagg ggatggttat gtccgattcg
    11881 gcttaattga aaataaagac agaataagac aggccttgag aaatttaaaa gctctgttta
    11941 aaagagatgg tttactaaag gctatttagt gcaatgaatg tcattataaa cacagagagc
    12001 gactgtgata gcaaagaggg caaattatta aaatcgcaag gtttggcagt acttaattta
    12061 ttagcatgcg gcggttatga ccttgtaaat cccccaatag gtaacttatt gaaatcgagc
    12121 catagtcttg agggagattg ggtggtttta actcctatgc attggcaagc atcacataat
    12181 gatgctgtca ttgtcgctct tgataaggat ttacgagtaa cagatgaaga ggtgaaatat
    12241 tggttcgatt tgtattctgc ctatttagcg gaagaaggag cgtcccttta tttttatgac
    12301 aaatacactt ggttattgcg agttgatgat aagccacctc ttaatgccaa accaatttat
    12361 caggtactaa acaagtcttt gatgccagaa ttatcgcaac tggatgagac tatgtactgg
    12421 caaaaatttt ttactgaaag tcaaatgttt ttctcttcca atgctcggaa atcattaatt
    12481 aatggtgttt gggcatgggg tagtgggaaa ttgaaaacta aaaaaacaac atcaatttgc
    12541 acagacaagc attttctggc agtagctcaa gcatattctt caaatgtgac tttatatgac
    12601 ccgtcagtga acgtgagcgg gtttgaggtt gttctattac atgacattga ttctttaagc
    12661 gaattacatc aagttgaaat aaaaaaaata ccggcacatt ggtactggaa caattgtgct
    12721 ttaattaaag ccaaatctca ttggtttact cgattatgga gaagtctgac gcatgctgat
    12781 taaacaacga caacagcctg agtttactgt taatttacct aatgtacctg atgtattaaa
    12841 acgtatttat gtaacacgag gcattagtga cgaatctcaa cttgataaac aattacaaac
    12901 attgcttcct tttcacactt taaaaggcat taatgaagct tgtctaagat tagagaatgc
    12961 tttgcgttca gagcaacgta ttcttattat cggtgatttt gatgccgatg gggcaacttc
    13021 tacggcatta gccattactg ccttgcgtgc tatgggtgca aggtttgttg agtacttggt
    13081 acctaatcga tttgaatttg gttatggtct tactcctgct attgttgaag tggcaagcaa
    13141 atggcaacct catttaatta tcactgttga taatggtata gccagttttg aaggagtaga
    13201 tgcggctaat aaaatgggaa tagatgtatt aattactgat catcatttac cagcagaatc
    13261 actacctgat gcttgtgcaa ttgttaatcc caatcagcct ggttgtttat ttccaagcaa
    13321 gtcaattgcg ggagttggcg ttatttttta cgttatgctg gcattgcgca ggcatctttc
    13381 caattgtaac tggtttaatg aaatcaacat gcctgagcct aatatggcga gttttctgga
    13441 tttggttgca ttagggactg tagccgacgt tgtggggctt gaccaaaata acaggatcat
    13501 ggttaatcaa ggcatgatgc gtattcgtca aggacaatgc agagagggaa taaaagcttt
    13561 gattgaaatt gctggtaggg attactccag gctaagagag tctgaccttg gttttgccat
    13621 agcgccaaga ttaaacgctg caggccgttt ggatgatatg tcgcttggta ttgaatgttt
    13681 gattacgacg gatccaaaaa tggcaagaaa tttctgtcaa cagttggatg agttgaatct
    13741 ggaaagaagg caaatagaat ctgaaatgaa agagcaagcc atgcttgctt tagagaaatt
    13801 gtcaattaac gatgaagaga ataagcactt accggttgcc ttgtgccttc atgataaaac
    13861 ctggcatcaa ggagtgatag gaatcttggc aggaaggatg aaggaaaaat atcatcgtcc
    13921 tgtcattgca tttgcttctg tcagtgagca tgagttaaag ggctcagcac gttctgtccc
    13981 cgatttaaac attagagatg tattggctgc tgttgataaa gacaatccag gactgattct
    14041 taaatttgga ggacatgcta tggctgctgg cttaagtatt agccccgaat tattgaatga
    14101 ttttcgctca gcattcgtgt cagaggttct gaaacatctt gacttatcac agtgcgaagg
    14161 agaaatactg acggatggac cattgcaatc ccatgaactg agtttggaaa cagcacaatt
    14221 aatacaacaa gctggtcctt ggggccaagc gtttgcagaa ccagtatttg ataatgtatt
    14281 tgaaattctg gatcaacgaa ttgtcggtaa aaatcattta aaaatgacat taatttcctc
    14341 acaaggaggg gagccactgg atgccattgc ttttaatgtg gatttaaaat cctggcctaa
    14401 tcataggact aagtacattc atgtcgccta taaactggat atcaattttt atcagggaag
    14461 aacaaggctc caattgctca tccaggcaat gaatcctgtt cagcaaggat aaactcttaa
    14521 attgatgcta tttttgtgta tttctcaaac aacaatgtat aatgttatat ttgctttaaa
    14581 ttgattaaac gtggttaata gtattttttc caaattatct atagcgccaa tgattgattg
    14641 gactaacagc ccatttcgta ctctgatgag gttattagct cctaacgccc ttttgtatac
    14701 tgaaatgcaa acaacagggg ctgtgcaaaa taatcctggc agagctttat attttaattc
    14761 tgttgaacat cctatcgcga ttcaattggg tggggcagat aaatccgcat tggttaagtg
    14821 cgccattatt gctgaacaaa aagggtttaa tgaaattaat ttgaatttgg gatgtcctag
    14881 tgacaaagta caggcaggac gatttggagc ctgcttgatg agggagccag aacacgttgc
    14941 tgattgtatt cgtgcaataa aagaggtagt aggaatacca gttacagcga aaacgagaat
    15001 cggtatcgat aaccaggaca gttatgaatt tttcagatct ttttctcttc acttggttga
    15061 agcaggctgt gacaaactga ttgttcatgc tcgcaaggct tggttaaatg ggttaaatcc
    15121 aaagcaaaat agaacgattc ctcccgtaaa ttatgattat gtatatgagc taaaaaagga
    15181 aattcctcat atctttatta caatcaatgg gaacatatta actattgaag aaataaacag
    15241 tcatttaatg tatgtcgatg gagtaatgtt aggacggctt gcttgcgata acccttatca
    15301 aattgctgaa attcatcaag ccttataccc agaattcatc aaaattaaac gtagccagtt
    15361 gttaatgaat tatctggatt attggtttga tcaatctcca gccaatctct cgttaagcat
    15421 tttggttaag ccacttttta atttagcttt tggattaccg ggagccaaac attggaaaaa
    15481 gaaattgatg aatattattc aggagaaaaa tacttccggt tttagtgagt taatccagta
    15541 cttattaaat ttggaagcaa taaatcaagt cgagtttgta taagaaataa tagagcagat
    15601 gtcaacattt cgtggaattt tatcaagatt tctagtacat taacgagttt attaattgga
    15661 acatcctgag ggttattgag gccatgctga gtacgttaat taagaaaatg tttggaagcc
    15721 gaaatgaacg gacattacgg cgaatggaaa agtcggtaat ggctattaat gcgtttgagc
    15781 cgaaaatgca ggctttatca aacgaagagc tggctggaaa aactcaagaa tttaaagaac
    15841 gtttcaataa tggtgaaagt ctcgatgagt tacttgcgga agcttttgct acggttagag
    15901 aagtatcatt gcgcactctc ggtcttagac attttgacgt acaattaata ggcgggatgg
    15961 tattgcatga gggaaatatt gctgaaatgc gtaccgggga aggtaaaaca ttggttgcaa
    16021 ccttgccagc ctatctcaat gccattagcg gacgtggcgt acatatagtc actgttaatg
    16081 attatcttgc gaaaagagat agccaatgga tgaggcccat atatgaattt ttagggctta
    16141 cagttggcgt tatctatcct gatatgtcgc acaaggaaaa acaggaagct tacaaagcag
    16201 atattgttta tggaactaac aacgaatacg gttttgacta tttacgtgac aatatggctt
    16261 ttagtctgac tgataaggta caaagagagc ttaattttgc tattgtcgac gaagtagatt
    16321 ccattttgat cgatgaagcg agaactccat tgatcatatc tggcgcggca gaagatagtt
    16381 cagagctgta tatcaaaatt aattctttga tcccacagtt aaaaaaacaa gaagaagagg
    16441 gtgacgaagg cgattatact gtagatgaaa agcaaaaaca ggctcatctg actgatgcag
    16501 gtcatctgca tatagaagaa ctgctgacca aagccaaatt attagatcct ggtgagagtc
    16561 tttatcatgc cagcaatatc atgttgatgc atcatgtgaa tgcggcactc aaggctcatg
    16621 caatgtttca cagagatatt gattatattg tcaaagacaa tcaagtggtg atagtggatg
    16681 agcataccgg ccgtaccatg cctggaagac gttggtcaga aggcttgcac caggcagtcg
    16741 aagccaagga aggagtccca attcaaaatg aaaatcagac tttggcttct ataacttttc
    16801 agaatttttt ccgcatgtat aataaattgt caggaatgac gggtactgct gacacagaag
    16861 cctatgagtt ccagcaaata tataatcttg aagtggttgt aatacctacc aataggtcaa
    16921 tgattagaaa agacgaggct gatttggttt atctaacaca ggcagataaa ttccaggcga
    16981 taatcgaaga tgtacgagaa tgtggtgttc gtagacaacc tgtcttagtt ggaacagttt
    17041 caatcgaagc ttctgagttt ttaagtcaac tcttaaaaaa ggagaacata aagcatcagg
    17101 tattgaatgc aaaattccat gaaaaagaag cccaaattat tgctgaagcc gggcgccctg
    17161 gcgctgtaac cattgcgact aacatggctg gacgaggaac agatattgtt ttaggtggta
    17221 gcttggcagc tgatctggct aatttgccag ctgatgcgag tgagcaagaa aaagaagcgg
    17281 ttaaaaaaga atggcaaaaa cgtcatgatg aggtgattgc agcaggcgga cttcgaatta
    17341 ttggctctga gcgtcacgag tcaagacgta tcgataatca attaagaggg cgagctggtc
    17401 gccaaggtga tcccggaagt agccgttttt atttgtcact tgaagacaat ttaatgagaa
    17461 tatttgcttc tgagcgcgtg gcttctatga tgcgtcgttt aggaatgcag ccaggtgagc
    17521 cgattgagca tagtttggtt accagagcaa ttgaaaatgc tcaacgcaaa ctggaagggc
    17581 atcattttga cgtaagaaag caattgttgg attatgataa tgtagcgaat gatcagcggc
    17641 aggtcattta tactcagcgt tcctctatta tggcaatgac ggatacacag gaagttgttg
    17701 aaatgatgag agaagaggta atggatagtc tggttgatac ttatatcccc cctcaaagtc
    17761 tggaggatca gtgggatcca caggctttgt ctgatgtttt gtcagatgaa tttaaaatca
    17821 aagcccctgt gcctgactgg attgataaag atcatagtat acaacctgag caaattaaag
    17881 aaaaaattct tgctttagct atagagcatt atgatgaaaa agtaagaaaa gttggtaggc
    17941 cagtcatttc acaatttgaa aaatcaatta ttttacaaac tttagataat cattggcgtg
    18001 aacacttggc tgcaatggat caattacgtc agggaattca tttaaggggt tatgctcaga
    18061 aggatccaaa gcaggaatac aaaaaagaag cttttagttt gtttacaatg atgctggata
    18121 atcttaagta tgaggtgatc aggatactat catccgttga aattcaaacg gaggaggatg
    18181 ctcaagttgt tgaagagcaa cgtagagcgg atcagattag aaaaatgaat ctcatgcatg
    18241 aaagtttatc agagaatgat gaggcaagtg aaacgcaaac ttttagacga caagaaaaga
    18301 aaattggccg taatgatcct tgcccctgtg gctcaggtaa aaaatataaa gcctgtcatg
    18361 gaagcctcgt gtgaaagtag cagttgctat cattatcgat gaaaaacaac gtatactaat
    18421 tacccaacgt ccttttcatg tcgcacatgg aggattttgg gagtttcctg gaggaaaact
    18481 ggagcctcat gaatctcctg aggatgcttt ggttcgagag attagggagg agttaggcat
    18541 aattgtaaat gaatatcgtt ttctggggta tgtcgattat gattatccag ataaacacat
    18601 acaattaatt atttttatgg taactcgttt tacaggcaac cctttatgtc aggaagggca
    18661 gctcaatatg aagtgggtaa aaaaagagga attaaatatt aatgatttcc ctaaagcaaa
    18721 tcatgcggtt ttcgatttaa ttaatatccc tgaacttcaa tatcaagctt tataaatgtg
    18781 cgaatagtct aaagtttgaa gctaggacat catatttaat tgctaagcac aaaatacact
    18841 ccttgtttag atgatgttat atcagatcag ttgtagagat caaatcaatc agaatggtca
    18901 ctgcaaggta tgttcacata gactagagat tgcaaagata gtagcctttt gtaaatgatt
    18961 agattttgta tttcaattaa ttgttaatca attggacaga aaaatgatta ctaagcaaaa
    19021 tggaatttat acacttaccc tttctttatt actattctca cttcaaatca acgctgctac
    19081 ggattatcaa acatatgaaa cagtttgtgg tgaaccaaca gatatgcttt cttttcttga
    19141 aagaggtggt attgcaacta atccttgtgt agtccctcct agaagcgtat taattacctc
    19201 tggatatcaa tatcagcaat taattggaga aggtgtacag cataattttc ctgctgctgc
    19261 aatacagtta ggtttgcctg gttattttga ggtagacttg ttgttaccca attatattaa
    19321 tcagacggtt gatccacgca taggttttag tcaaactcag cttattgtca atcatgttct
    19381 atggtttaac aataagtggg ttattactgc tagcggaaca tttgtatttc catcaggcag
    19441 cgcttctttt ggtagtccaa atccaggagg tggtgtaata ggaattttaa gttataactt
    19501 taattcccaa ttaaatctta caagtaattt gggtattacc actcaatcgg agcctagtta
    19561 cgatggtgga caaagctata cgagtgttaa tccagatctt attttatcgt ggactaaaaa
    19621 taaaatatca ttatttgctg agatttatgg gcaaagtaaa acagcacctg atgaaggaag
    19681 cggttttagt actgatgcag gagttcttta tcaagttaaa aagaatattg taattgattt
    19741 tgaggttgat caacgtatca ctggcctttt gaatggtgtt gagcgttatt atggcggagg
    19801 aattactata caatttaatt aatgcggctt cactttaacg caaagccagc atagcgcaca
    19861 gggtgtctat atttggcaaa atgcaatttc catttctatt gttttatctc tgatttcttg
    19921 accagtgaca agctcataaa gcctgatagt taaactatga tgccctaact ggattttggg
    19981 tatcattttc aatttcttat ctattcttaa caagatcaga tggttcagtg atttaggtga
    20041 aagactatgc tgatagaatc cattatttgc tctaatggga gtatactgag taacacctcg
    20101 taacaatgac aaataaattt ttacagtatc ttccagatcc ttcaggcatg atacccattg
    20161 actaatggtt ttttgtctca ttaggggatc tgaatcaaac cacaatacca aatgagggga
    20221 attaaattcg cattctttag tattggggtg gtgtatttgt cttaaagttt ttaaaaattc
    20281 atcatcatgg attttgttac taaatcgacc tggtacatga tttaacacat gaatttgtgt
    20341 ttccagatca tcaaataatt cactatgatt caatagatta gcttttttaa gtacatgctc
    20401 aattctaatt aactctttga taaatctgct ttttaattcc ggcttttcaa ctatttcaac
    20461 aatctcaatt atattcttta atgcaaatcg atgaatcacc tcatgagact cattacaagc
    20521 ttcattaatg gttttaaaaa ggtactccag gcgcaaggca attcttgata aaaaatgagt
    20581 tgctagttga aacgttatca tatggtcatg catagattaa ggccatcctt agctttatcc
    20641 tataataatg gcaatttaaa tttttataga tgcacattgg gtaaaaatgg gctcctgggt
    20701 ttatttaaaa acaatattgt tttttataaa caaatctaaa aaatcaagct attaaggtga
    20761 attttgtgtt aagactaaca taaaagttgt tgaaaataaa aaaatcttta aaatccatat
    20821 attaactggt cttttgctag aaatttattg ttactgtttg atttttgctt ccctaagata
    20881 tttttgatga agcttattca ccttagcttt taattcactc aatccagatt cattgatcag
    20941 cacatcatcc gctgcttcca agcgttgctc taaattaggt tgtgtggcta aaatagctaa
    21001 tgcttgcttt tttgtgcaat gatctctttt aacaattctg tctagctgag attccagggg
    21061 agcaataacc aatagaactt tttgaagata ggggtagtga tgtttattaa ataacaaagg
    21121 aatttcaata aggcaatagg gcgaagtgca ggcaatgagt tgctcttcaa ttttttcacg
    21181 gatgactgga tgtaatagac tttcaagcca taagcgctca tttgaattag aaaatataat
    21241 atctcttata cgtttccgat caagctcacc attattaaga accactgaag aaccaaaatg
    21301 tgaaataata tcctggtagc agggtgtatt tttagaagtt aattctttgg caattttatc
    21361 tgcataaata acatttatac ccagctcgga aaaaaattct gcgactgtac ttttgccact
    21421 ggcaatatta ccagttagtc ccactgaata aaccatctga gtgggctcct tcatttttgt
    21481 ttgcacaagt taccatatta atataacaag tagcaggtag tgtactgttt tctttacaat
    21541 aggcctgggc agctaaggcg gcatcatccc gatttgagta aaaattactt tcccaaggtt
    21601 gagcagtaat atcgatggct gtgcagcgcc acattggtct ggtgcttact ccatttataa
    21661 atccttcaca atttgatatg gatgtcttac atgttgcggg aagttggctt tcttttttgc
    21721 aagcagcaaa tgcaacattg atagccacct ttcggtatga gttttgcgcc gtccatgctt
    21781 tgttggcttt atcataagtt acacatttcc aataacttcc ttttttcatc attggggcag
    21841 taaagccatg atgggataaa ccaattaata gtagtaaacc ataaaaaata taattcctca
    21901 tattttttct cctttaagca aggctgttaa ttcagcaatg gatagccctt tggaataata
    21961 ccagccttgt tgtaaacgaa caccattatt tgccaggtaa ttagcttggt cttttgtttc
    22021 cacgccttct gctatagtaa ctaagtttaa tccttttgcc atctgtataa tggcgtcatt
    22081 taatgattcg ataatatcat tagaaccaat tgcttgaaca aataatttat caatttttaa
    22141 ataattgaat ggaaaatgtt gcaaataact aatgctggca tggccagtcc cataatcatc
    22201 aacagcaagt gaaaaacctg cttgccttag ttcttgc
//
LOCUS       DAXLDT010000046        22163 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXLDT010000046 DAXLDT010000000
VERSION     DAXLDT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22163
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.046"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..196
                     /locus_tag="ACF2SP_001345"
     CDS             <1..196
                     /locus_tag="ACF2SP_001345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359138.1"
                     /translation="CHLLSPLLENIVFRSNNSDNQPILDALALIKKYFDSNKTYFPSN
                     EEVPLDCLPDKWKKTYYSSN"
     gene            589..861
                     /locus_tag="ACF2SP_001346"
     CDS             589..861
                     /locus_tag="ACF2SP_001346"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFD2359139.1"
                     /translation="MIKEVDLENQFTHDFISYNQKIYLKPNEISERSLLFIYGMGTNV
                     GLKHMCAGNAHVSEYQLRYIKNYFLSTDNLKNALSKVANALFKIRL"
     gene            877..1119
                     /locus_tag="ACF2SP_001347"
     CDS             877..1119
                     /locus_tag="ACF2SP_001347"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFD2359140.1"
                     /translation="MPIAVAGDSKQISAYFQNLISECHNRFGGRGVMIYWHVEKNACC
                     IHSQLKSVSSSGVSAMIEGILGSVDIHFNQRAVAAI"
     gene            complement(1095..1825)
                     /locus_tag="ACF2SP_001348"
     CDS             complement(join(1095..1507,1507..1825))
                     /locus_tag="ACF2SP_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611567.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFD2359141.1"
                     /translation="MLTDDEWNRIKDCLPGKPGDPGRRGADNRLFIDGIMWMARNGAK
                     WRSLPEEYGKWSTVHKRFIRWSKKGIWQMIFNTLAVDTDTEWLMIDSTIIRAHQHAAG
                     AKGGKEKQALGRSRGGFSTKIHALCDALGNPLKFILTPGQTSDFTQALTLLEGKTTTA
                     ILADKGYDADYIINAAIDMNAVVIIPPKSHRKVLREYDKDIYKERNLIERMFNKLKHF
                     RRVATRYDKLAQSFLSFVYIAATAL"
     gene            1870..2223
                     /locus_tag="ACF2SP_001349"
     CDS             1870..2223
                     /locus_tag="ACF2SP_001349"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFD2359142.1"
                     /translation="MHNHECRQNLRHCTEMSVQENYVDTHGQSEVGLTISHLLGFSLM
                     PRLANLNKQKLAQCELGDYQKYKNLQPVLTQTINWQLTDVDLTNCRIKINQGKGKKIE
                     LSLFRQHLKKLWLSI"
     gene            2220..2387
                     /locus_tag="ACF2SP_001350"
     CDS             2220..2387
                     /locus_tag="ACF2SP_001350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2359143.1"
                     /translation="MKEYKNENRLFLFESGFRRHYTDCGIRKILMRYTKLAGIERSIS
                     PHKLRHFFYLA"
     gene            complement(2608..4608)
                     /locus_tag="ACF2SP_001351"
     CDS             complement(2608..4608)
                     /locus_tag="ACF2SP_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFD2359144.1"
                     /translation="MSDTPFIAANKKVAELTFRVIILAIILTVLLAMSNAYLALKLGI
                     LTSASIPAAIISMGILRFFKNSTILENNAVQTAASAGEAVAGGIVYTIPALIIIGFWN
                     HFDYVTNFFIAVCGGILGVLFSIPLRRILVNDQTLKFPEGRAIAEVLKSSADKVGIKD
                     IFLGGLVGGLLELLQIGFKVIASSWNYWFVVKRSLFGLGAGFSATMIGAGYLVGHDMA
                     ISIFLGAVISWLIALPIASQFYPDFLNQYPAEQAATLLWNSEMRYLGIGAMLFAGIWT
                     FIKLVKPLTRSVKTSFRTYISKSKFREQYPRTEKDIPMPFILMGIGLMAGILFLFFQI
                     IFPLGQVGLDNDFSPTIVFIGVIYVLVVGFLFSVITAYFSGMVGVTASPGSSVVISGM
                     LFAAWLLVSAINHLLPLPLTSHQIQAAEAITIIIGAVVTGIAAIANDNTQDLKVGQLV
                     GATPWKQEVMLLLGVVISALVIPPVMQILFNVYGIAGVMPHAGMDVTQSLPAPTAALM
                     AAITEAVFRNSLPWNMMLAGSCIILLAIFLNRILNIKRFLNLSILGIAIGMYLPLSSS
                     VPLFIGGMIALFVQKQLGRVKIASEESMIRKQKGTLIACGLVAGSAIMDVLLAIPFSI
                     FHSPDALQLVGTSWKQYGVYLGVFSTLLLAWWISHRVCRSKI"
     gene            complement(4608..5303)
                     /locus_tag="ACF2SP_001352"
     CDS             complement(4608..5303)
                     /locus_tag="ACF2SP_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HFD2359145.1"
                     /translation="MPIQMNLINKIGLVFGLLFYSFLASAITTEVIKKETAEYLLDIK
                     YPQGFQSNGVNQAVKEFITNQQKSFMNELSEDADTPADAPGKTGLNITYSIPYQSTNA
                     LSVRFNISIYHRGAAHPSNAVKVLNFIKNQPVQLSDLFVPGSDYLQPIADLAKKEITA
                     KKISDENWIKEGTKPTQENYSIWHFTKKGIAIVFNTYQVAAYVYGEQTVDIPLSLISA
                     MVKPEISKAVWGN"
     gene            complement(5330..6541)
                     /gene="hutI"
                     /locus_tag="ACF2SP_001353"
     CDS             complement(5330..6541)
                     /gene="hutI"
                     /locus_tag="ACF2SP_001353"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HFD2359146.1"
                     /translation="MFACDELLLNASTIDATGLQLSNQAIVIRKGRIEWCGSEDQLPA
                     HFQESAKSRKDCHGQLITPGLIDCHTHLVYAGHRAAEFRLKLQGVSYADIAKSGGGIL
                     STVQMTRDASEEELVDQSLPRLLALKNEGVTTVEIKSGYGLDLQNELKMLRVARQLGE
                     VAGIRVKTTFLGAHAVGPEFKGNSQAYVDFLCNEMLPAAKNMDLVDAVDVFCESIAFS
                     IRQAEQIFQAAKDLNLPIKCHAEQLSNMGASSLAARYGALSCDHLEFLDENGALNMVK
                     ANTVAVLLPGAFYFLKEKQKPPVDLLRQVGVGMAIATDSNPGSSPTTSLLLMMSMACQ
                     FFSMSIPEVLSAVTYQASRALGMEKDIGSIEAGKIADLVLWSIKDSAALCYYFAYPLP
                     HQTMVAGEWVS"
     gene            complement(6541..7350)
                     /locus_tag="ACF2SP_001354"
     CDS             complement(6541..7350)
                     /locus_tag="ACF2SP_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2359147.1"
                     /translation="MSGIISPIVFYIDEEIVYLITGGGSGIGRALAFALAKRNKQVFI
                     VGRREKLLQETASLSANIHYLCADVSTPQGRELIKASLSATPQIDGLINNAGTLEPTL
                     LLKDISFAEWHQALNTNIDAALFLPQLLYDKLSGGRVLNVGSAAAYFPIKGWGAYCVS
                     KAALAMLTRCWQIECDSLAFASVMPGIIDTHMQAIARNGINLDPEQVNFYRRLKDHNR
                     LISPETVAEFFIWLLLDIDNETYVSKEWDIYDTTHHSAWLKPPHQVLHWEF"
     gene            7640..8599
                     /gene="hutG"
                     /locus_tag="ACF2SP_001355"
     CDS             7640..8599
                     /gene="hutG"
                     /locus_tag="ACF2SP_001355"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HFD2359148.1"
                     /translation="MFDDLSNYRPANPALWQGRMDTANQERFFQKITFIDNQNELMTK
                     DKKTIFLGFASDAGIKRNLGRTGAKLGPDQIKTQLAKLPCHNNKHYVDLGNVVCENDE
                     LELSQSQFAQIVHFCHENGHQICAFGGGHEIAWAHYQGLSSLYPKLGVINFDAHFDLR
                     PYKKGEFGNSGTPFSQIATYCEEKKMPFHYCCIGVQKFGNTPSLFEKAKELNVSYLSA
                     EDLYEQSQAWQIAFLDDFILNLDHIYLTICLDVLAECYAPGVSAPQALGLSPWQIMPL
                     LKYLIQSGKVVSLDIAELSPPLDSELKTARLAALIIAELLDTN"
     gene            8612..9586
                     /locus_tag="ACF2SP_001356"
     CDS             8612..9586
                     /locus_tag="ACF2SP_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFD2359149.1"
                     /translation="MKKTVLWGALFTLITTQANSEATQQAISTQSSASTGTNVVSEST
                     NQVPATNQNTQQNQLNQPVQSNGQQPTQTIQNNQPNQPSQTGEQQSNQNTQNNQQNQP
                     AQQQTTQQPAQQQVPSQQTAPVINCDYKISAETKAIEQSLVLTWSEKAIIQSFDFDPA
                     TVDAQLQKLQACFTDQGWLSFNSALQKSGNLDAIKSQKLHVSSQIDGQPQVTEAIENQ
                     WKIIIPLQVVYQNDKEKVTQLLNVNVTVGRKITGDLGINQMIAVPRTATNTSQTNSPS
                     NTNPADANTSTPQTPGDTTNNATQTNTQTQTGTPTNSPPSGSTTNTQN"
     gene            9643..10815
                     /locus_tag="ACF2SP_001357"
     CDS             9643..10815
                     /locus_tag="ACF2SP_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFD2359150.1"
                     /translation="MRLIFTGVLCLLTLNAQANVIQYFAGISYNNPADLFKVKNGILL
                     IGGTGSYADLQFKGSALNFNTFQYESGVNHSRTYIVWPYGRVAKRLNDKTVVAVDLTE
                     PFNSNLDWGNDAFTRYAATQNYLTDVDLSPKISYAISKKLQIGGGINFNVLLKNEVNW
                     AFPTGQSTYANLINRSSSFGVGYNLGINYAVNDTNFLGITYYSRIRQNTSGTSYLGLA
                     ANPDFQFGFYMPATTVASYVHIFNPKWLINLQVFQSEWNANQKVRLYNTAAPPPFTNF
                     IFDMHFDASYAYLAAIRKQVSDKLGIALAGMIDDGPEEDGLRTIVFPSDTQYFLGLIG
                     DYRFTEHASMELILGHVYSNPSIQNKAKVNNVPVPFTTGRVTINANVLDLKVKIEG"
     gene            complement(10855..11280)
                     /locus_tag="ACF2SP_001358"
     CDS             complement(10855..11280)
                     /locus_tag="ACF2SP_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HFD2359151.1"
                     /translation="MYNIMSFLCGLLFGAGLTISNMINPNKVLNFLDINGFWDPSLLI
                     VMAVAVTVTFLGYKLINKFDKPILCNQFYLPEKKNIEKKLILGSIIFGIGWGVAGYCP
                     GPSITALTTFNSDPVYFVIGLIAGSWTYYWLFKKRTIQK"
     gene            complement(11280..11717)
                     /locus_tag="ACF2SP_001359"
     CDS             complement(11280..11717)
                     /locus_tag="ACF2SP_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HFD2359152.1"
                     /translation="MDYITSFTPYLSAIGGLLIGLSAVLFLWLNGRIAGISGMIHGLF
                     PPEKPFPAWRIAFLLGLAAAGLIYFISPVIQFPLRTHYPVFLLLISGFLVGFGTRMGQ
                     GCTSGHGVCGLARFSKRSFIATLLFILSAMITVYLIRHVGGLY"
     gene            complement(11827..12438)
                     /locus_tag="ACF2SP_001360"
     CDS             complement(11827..12438)
                     /locus_tag="ACF2SP_001360"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFD2359153.1"
                     /translation="MKKIYWAVILMCLGLTSCVEFDESTLITDDAGYVHRTVHYLRDK
                     RGRNYFPMQIKAPGERLFVFDPKASAWAAYDEEGHRVMTGAGSGGIDVCEENPNQSCR
                     TITGTFRIYNKRGIDCRSGEYPVDTKGGAKMPYCMYFYRGFTIHAAYEVPEHPSSHGC
                     VRVFPSAAKWLNEEFLRVGTKVIVLAYPDQNGVKTMSSLEKVN"
     gene            complement(12480..14153)
                     /gene="ettA"
                     /locus_tag="ACF2SP_001361"
     CDS             complement(12480..14153)
                     /gene="ettA"
                     /locus_tag="ACF2SP_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFD2359154.1"
                     /translation="MAQYIFTMNRVSKIVENQRFILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDTQYEGEARPQPGIKIGYLAQEPELDLNKTVREIVEEGVAEIKEKLS
                     RFDAISMRFAEPMSDDEMNTLLMEQGELQNEIEACGGWDLERKLDVAADALRLPEWDA
                     IIGKLSGGERRRVALCRLLLSSPDMLLLDEPTNHLDAESVAWLEHYLEEFSGTVVAIT
                     HDRYFLDNAAEWILELDRGEGIPYKGNYSSWLEQKEARLSMEKKQEDALQRAIKAELE
                     WVRTSPKGRHAKNKARLARFEEMNSKEFQKRNETNEIYIPPGERLGDLVLEGEKICKS
                     YGDRILIDNFDFKLPKGGILGIIGPNGAGKSTFLKMLTGQEEPDKGVIRIGETVKLAY
                     VDQMRDNLNPNNSVWQEISDGHDIMQIGNFQMPSRAYVGRFNFKGTDQQKKISQLSGG
                     ERNRVHLAKLLKSGGNVLLLDEPSNDLDVETLRALEEAILNFPGCAIVISHDRWFLDR
                     ICTHLMAFEGDSQITFFEGNYTEYETDRKRRLGDEANRPSRIKYRKLSD"
     gene            complement(14196..15218)
                     /gene="tdh"
                     /locus_tag="ACF2SP_001362"
     CDS             complement(14196..15218)
                     /gene="tdh"
                     /locus_tag="ACF2SP_001362"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFD2359155.1"
                     /translation="MKSLVKAKKEPGIWMQDIPVPEYGVNDVLIKIKRTAICGTDIHI
                     YSWDEWAQATIPVPMTVGHEFYGEIVEVGKEVQGLKVGQRVSGEGHITCGFCRNCRAG
                     KRHLCRNTLGVGVNRPGCFAEYLALPATNVIALPDNITEEQAAILDPFGNAAHCALAF
                     DVVGEDVLITGAGPIGIMAAAIVRHIGARHVVITDVNDHRLELARQMGVSRAVNVKYQ
                     KLIDVANELGMLEGFDVGLEMSGNPMALNDMMKAMNHGGHVALLGIPPQETPIDWNQV
                     IFKGLVIKGIYGREMFETWYKMIAMLQSGLNISPVITHNFPVDEYQHAFQIMASGQSG
                     KVILNW"
     gene            complement(15305..16489)
                     /locus_tag="ACF2SP_001363"
     CDS             complement(15305..16489)
                     /locus_tag="ACF2SP_001363"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFD2359156.1"
                     /translation="MLERFINYLQNELETLKADGLYKSERVISSQQQADVKVNEREVI
                     NLCANNYLGLANDPELIAEGQAALAKYGYGMASVRFICGTQTPHKQLEQKISHFLSQE
                     DTILYSSCFDANTGLFETLLTEEDAIISDALNHASIIDGVRLCKAARYRYANNDMKAL
                     EEQLIAAKNARFRLIATDGVFSMDGILANLPAICELADKYDAMVMVDDSHAVGFMGKT
                     GRGTPEHFGVSDRIDIVTGTLGKALGGASGGYTASNQVVIDWLRQRSRPYLFSNTLAP
                     VIAHTSCVVLDKLSTNNSLAEKLKRNSHYFREGMTQLGFELIPGEHPIIPVMLGDASL
                     AGRIANRLLDLGVYVVGFSYPVVPKGLARIRTQMSAALELHHLDKALKAFETVGKEFS
                     VI"
     gene            16670..17320
                     /locus_tag="ACF2SP_001364"
     CDS             16670..17320
                     /locus_tag="ACF2SP_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFD2359157.1"
                     /translation="MNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHF
                     AYSDMQIPLAYGQRMLTPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVI
                     SIDYYSEFTANAKRKLEEHNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHK
                     LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF"
     gene            17332..18699
                     /locus_tag="ACF2SP_001365"
     CDS             17332..18699
                     /locus_tag="ACF2SP_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFD2359158.1"
                     /translation="MRKSLFCWILTLGVSTHVFATDLMDIYQQALENDTIFKEAYDTY
                     MSSTEAIPQARAALYPQVGLGSQAGRNYQDAVAGAFSANQYYGSYLWQVNASQALFNY
                     QAWAKVAQAKASVKAAQATFNDAAQNLILRTAKAYFDVLFAKDTLDFAEAKKRANKRQ
                     YDQATQRFQVGLDAITSVYEAKAAYDQSIATVIAARNNQINQSENLRKLTNHVYETLA
                     PLKDSKIPLVKPEPNDVNQWIDTGLKQNYKLYAAKYNLEVAKDNVKAISAGNWPVFSL
                     QSNASQVHNNASGNTVFIPSKQTQANIAIAMNFPVFQGGLVQAQTRQAQYGFQSTSEK
                     LEQTYRDVIVNSRIAFNTITDGISKVKADRQTVISVQNSLQSTEAQFEVGTRTMVDVV
                     NAQQRLFEAQEQLARDQYDLINSILTLKYLAGTLNVNDLEQINSWLATTRVNGFSPVD
                     NKTSK"
     gene            18717..19568
                     /gene="ttcA"
                     /locus_tag="ACF2SP_001366"
     CDS             18717..19568
                     /gene="ttcA"
                     /locus_tag="ACF2SP_001366"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFD2359159.1"
                     /translation="MSSNPSSVEKKLLHYTGKAIADFNMIQRGDRVMVCLSGGKDSFT
                     LLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVK
                     EKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
                     MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLI
                     DQLAIENPKVPSNMLHALQSLKPSQLMDQNFWNFKNLEDGLETAQSIQCEEVFNAQEF
                     EIEDEKI"
     gene            19758..22064
                     /locus_tag="ACF2SP_001367"
     CDS             19758..22064
                     /locus_tag="ACF2SP_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFD2359160.1"
                     /translation="MIDRNIVNLRLFRIYSKRYLKFDFVAAIVVFLVAIPLCLGIALA
                     SGAPLFSGILSGIIGGIVVGIFSGSQVSVSGPAAGMAAVVLAAISQLGDFNTFLLALT
                     IAGLLQMIIGALRAGFVADYVPSNVVQGLLCSIGILLIIKQLPLAFTLSSDFDELKTH
                     LLETTEGFTVSPLLALSQHINEGALIITTLSLAILIYFDITKNKILKEIPAPILVVLA
                     GILLNELFIWTNSSLAQNSPQLVNIPDTNGFFQFFSHLEYPDWSAWTNPKVYLYALVI
                     CIVASLETLLNLKASERLDKKRRHSPTNQELVAQGLGNITSGLVGGIPVTSVIVRTSI
                     NIHAGSKTKFSAVLHGFFILFAVMLIPGALNKIPLSSLAAILIYTGYKLNKPAIYINI
                     FSQGSDRFIPFIVTVISIIAFNLLAGILIGLAISLFYILKSNSQARIDIIKEIYPNGS
                     TYRLMLPQQMTFLNKAALVAELDTLPRRSQLIIDARHSQYIDKEILDLFNELKEELAG
                     SKQISVNFTGFQEHYKIHNYIDFITVTTYDVQTNLTPAQVLNILLEGNQRFLSDNRIH
                     RSNQIDIKYTAKTQHPIAVVLACIDSRVPVETIFDMSFGDLFCVRIAGNVINDDILAS
                     IEYACNVVGAKLIMVLGHTRCGAIQSACDGIEKGHITQLLSKIKPAVNAEKETTTERN
                     GKNQTFVNHVTELNVANTLQNIYKKSDILRTMIDSNEIGMVGAIYDVSSGKVSCKTYT
                     EELSTLDGDENKLLAQKFETILLEAKAS"
BASE COUNT         7070 a         4741 c         3933 g         6419 t
ORIGIN      
        1 atgccattta ttatcgcctt tattagaaaa tatcgttttt cgctccaata attcagataa
       61 tcaaccaata ctcgatgcat tggccctcat taaaaaatat tttgacagca acaaaactta
      121 ttttccaagc aacgaagagg tgcctctaga ttgtttacca gataaatgga aaaaaacata
      181 ttattcatcc aactagtgaa aaattaaacg tatttattat gaggtctatt tactaaaaaa
      241 acttgcagac aggattagat gccgtgaaat atggattgaa tcaccattta aacaccgaaa
      301 cccagatgag gatttgccaa ataattttga agaagacaaa gaagtgtatt ttgatgaact
      361 ttctttgtcc cttgacggcg atgtatttat tgaacaatta aaacagcgcc atacaaatgc
      421 atgagctgct ttaaatgata atattcccaa aaatacgaaa gttcgaatta acccccaaaa
      481 tggcggtcgt attatcgtaa cccctcttac accccagata gaatcaaaca atattggaat
      541 tataaaaacc atttgcagga aaaatgggaa ggaaccaatc ttattgatat gatcaaagag
      601 gtcgatttag aaaaccagtt tactcatgat tttatttcct ataaccaaaa aatttattta
      661 aaacccaatg aaatttcaga aaggagctta ttgtttattt atggaatggg aactaatgtt
      721 gggttaaaac atatgtgtgc gggaaatgct catgtaagtg aataccagct ccgatacatc
      781 aaaaattact ttttatccac tgataactta aaaaacgcgc taagcaaagt ggcaaacgct
      841 ttattcaaaa taaggttata agatatatgg agcgatatgc ctatagctgt tgccggtgat
      901 tcaaagcaaa tttcggctta ttttcaaaat ttgatttcgg aatgccataa tcgcttcggg
      961 ggaagaggcg tgatgattta ctggcatgtt gaaaaaaacg cctgttgcat ccactctcaa
     1021 ttaaaaagcg tatcaagctc tggggtttct gccatgattg aaggtatact aggttctgtc
     1081 gacattcatt ttaatcaaag ggcggtggca gcaatataaa cgaacgataa aaaagattga
     1141 gcaagtttgt catatcgtgt tgccactctt ctaaaatgtt tgagtttatt aaacatgcgt
     1201 tcgatgaggt tccgttcctt ataaatatct ttgtcatact ccctgagcac ttttcgatga
     1261 gattttggcg gtatgatcac aacagcattc atgtctatgg cagcgttgat aatgtaatcc
     1321 gcatcataac ctttgtctgc gagtatagcg gtagtcgtct ttccttcaag caatgtcagt
     1381 gcttgtgtaa aatctgaagt ctgtcctggt gtaaggatga atttaagagg attaccaaga
     1441 gcatcacaca atgcgtgtat tttggtagaa aaaccgcctc tggagcgacc taatgcctgt
     1501 ttttcttgcc cccttttgct ccggcagcat gttgatgggc acgaataatc gttgaatcta
     1561 tcatcagcca ctctgtatca gtgtcgacgg ccaatgtatt aaatatcatt tgccagattc
     1621 ctttcttgga ccaacgtata aatcgtttat ggactgtaga ccatttacca tattcctcgg
     1681 gcaatgacct ccattttgca ccattccggg ccatccacat tataccgtct ataaaaagcc
     1741 ggttatctgc tcccctccga ccaggatctc ccggcttacc tggtaagcaa tctttaatcc
     1801 tgttccattc atcatctgta agcataaaaa aaccactaaa aaactaccat gtcatttcaa
     1861 tagcagatta tacacaatca tgaatgtcga cagaacctaa gacactgtac agaaatgtca
     1921 gttcaagaaa attatgtcga tactcatggc caaagtgagg ttggccttac catttcacat
     1981 cttctaggat tttctttaat gccacgactg gcgaatttga acaaacaaaa actggcacaa
     2041 tgtgaacttg gagattatca aaagtataaa aatctacaac cggtgcttac acaaactatt
     2101 aattggcaat taactgatgt tgatttaacc aattgtcgaa taaaaattaa tcagggtaaa
     2161 ggaaaaaaga tagagttgtc cctttttcgc cagcatctaa agaaactttg gctctccata
     2221 tgaaagaata taaaaatgaa aacagactat ttttatttga gtcaggattt agacgccact
     2281 atactgattg tggaattaga aaaattttga tgagatatac taagcttgct ggtattgagc
     2341 ggtctatctc acctcataag ttaaggcatt ttttttacct ggcttaagaa gaaaaatatc
     2401 gatgatgctt ttattcaacc ctactcaggt catgctaaaa gagactctct ggaaatctat
     2461 tccaaattaa gtttggccga tgcacaagat aagtataatg atgttattgg ggattttccg
     2521 atctcatatt ttaaattttt gcgtcagggg gcaagttccg gagcactagc ccaataagta
     2581 gaattgttag agtaattatc aattaactca aattttgctt ctgcagacac gatgacttat
     2641 ccaccatgct aaaagcaaag tagaaaatac tcctaaatac actccatatt gtttccatga
     2701 tgttcctacc agctgcaaag catcagggct atgaaaaata gaaaacggta tagccagtaa
     2761 cacatccata atcgctgatc cagcgaccag accacaggca ataagagtgc ccttttgttt
     2821 cctaatcata gactcttcag acgctatttt cacacgtcct aattgctttt gaacaaatag
     2881 ggcaatcatt cctccaataa ataaagggac cgaagaggaa agaggtaaat acatgccaat
     2941 ggcaatacct aaaatagata aattaagaaa gcgtttgatg tttaatatgc gattgagaaa
     3001 aatagccaat aaaataatac atgaaccagc caacatcata ttccagggca atgaatttct
     3061 aaatactgct tccgtgatgg ctgccattaa tgcggcagtc ggggcaggta acgattgtgt
     3121 tacatccatt ccagcatgtg gcattactcc tgctatgcca tagacattaa ataaaatctg
     3181 catgactgga ggaataacca aagcagaaat aacaactcca agcaacaaca tcacctcctg
     3241 tttccatgga gtagccccaa ctaattgacc tacttttaag tcttgggtgt tgtcgtttgc
     3301 gatagccgct atgcctgtga ctacagcacc tataataata gtaatagctt ctgcagcttg
     3361 aatttgatgt gaagttaatg gaagtggcaa tagatgatta attgctgaaa ccaataacca
     3421 tgcggcaaaa agcatacccg agataaccac tgaactccca ggacttgcag tgactcctac
     3481 cattcctgaa aaataagctg taatcactga gaataaaaaa ccgacgacta aaacataaat
     3541 aacaccaata aatacaatag ttggtgaaaa atcattatcc agtccaacct gccctaatgg
     3601 aaaaatgatt tgaaagaaca aaaacagtat tcctgccatc agtccaattc ccatcaatat
     3661 aaatggcatg ggaatatctt tttcagtacg aggatattgt tctctgaact tgctcttgga
     3721 aatataagtt ctgaatgatg tcttgacact tctggtgagt ggtttcacta attttataaa
     3781 agtccaaata ccagcaaaca gcatggctcc tatccccaaa tagcgcattt cactattcca
     3841 taacaaagtg gctgcttgtt cggcgggata ttgatttaaa aagtcaggat aaaactggct
     3901 ggctattggc aaagcgatca accatgaaat gactgcccca agaaaaatgc ttattgccat
     3961 atcgtgacca acgaggtagc ccgctcctat catggttgca gaaaagccag cgcccaaacc
     4021 aaatagagaa cgttttacaa caaaccaata attccagctg ctggcgatta ctttgaaacc
     4081 aatttgcagt aactcgagaa gtccgcctac aagtccaccg agaaaaatgt ctttaatacc
     4141 aaccttatct gccgaagatt tcaagacctc tgcaatcgct cttccttctg gaaatttgag
     4201 ggtttggtca ttaacaagaa ttcgacgcaa aggaatagaa aacaaaactc ccaggattcc
     4261 tccacaaaca gcaataaaaa aattagtgac gtaatcaaag tggttccaaa acccgatgat
     4321 aatgagcgca ggaatagtgt aaacgatacc gcctgcaaca gcctcacccg cagatgctgc
     4381 tgtttgtacg gcattgtttt caagaatggt tgaattttta aagaaccgca aaatccccat
     4441 ggaaataata gcggcgggaa ttgaagcgga agttaatata ccgagtttaa gcgccagata
     4501 agcattagac atggccagta gaacggtcaa aattatcgcc agaataatca ctctaaaagt
     4561 gagttcagct acttttttat tcgctgcaat aaatggagta tcagacatca attaccccac
     4621 actgctttgg aaatttctgg ttttaccata gcagaaatta gtgataatgg gatatccact
     4681 gtttgctctc cataaacata agctgccacc tggtatgtat taaaaacaat agcaatccct
     4741 tttttggtaa aatgccaaat actataattt tcctgtgtcg gtttcgttcc ttctttaatc
     4801 cagttttcat ctgaaatctt cttggctgtg atttcttttt tagccagatc agcgattggc
     4861 tgcaaataat cggaacctgg aacaaataaa tcagacaatt gtacaggctg attcttgata
     4921 aagttgagta cctttactgc atttgaggga tgagcagctc ctcgatgata tatggaaata
     4981 ttaaatcgaa cacttagggc attagttgat tggtaaggaa tagaatatgt aatatttaaa
     5041 ccggttttac ctggagcatc tgcaggagta tcagcatctt cagataattc attcataaaa
     5101 ctcttttgct gattagtaat aaattccttt accgcctgat ttactccgtt actttgaaat
     5161 ccctgagggt attttatatc cagtaaatac tccgctgttt cttttttaat aacttctgtt
     5221 gtaatcgcag aagcaagaaa cgaataaaat aatagcccaa acaccaggcc aattttattg
     5281 attaaattca tttgtatcgg cattgtttat ctccaatcaa gtaatcctct caggataccc
     5341 attcaccagc caccatcgtt tgatgaggta acggataagc gaaataataa catagcgcag
     5401 cactatcttt tatagaccat aaaaccaaat cagcaatctt gcctgcttca atgctcccaa
     5461 tatccttttc cattcctaaa gctctggaag cctgatacgt cactgcagat aaaacttcag
     5521 gtatagacat agagaaaaat tggcaagcca tactcatcat caataacaaa gaagtcgttg
     5581 gagaggaacc cgggttagaa tccgtggcaa tggccatacc aacaccaacc tgacgcaata
     5641 aatcaacagg tggtttttgt ttttctttaa ggaaataaaa agcgccagga agtaagacgg
     5701 caaccgtatt ggcttttacc atatttaacg cgccgttttc atccaaaaac tccagatgat
     5761 cacaggataa cgcaccataa cgagcagcca aagaactggc ccccatgtta gataattgtt
     5821 cagcatggca ttttattggt aaatttaaat ccttagcggc ttgaaaaatt tgctcagcct
     5881 gccttataga aaaagctatg gattcacaaa aaacatccac ggcatctacc aaatccatat
     5941 ttttagctgc aggcaacatc tcattacaaa gaaaatcgac ataagcctga ctatttcctt
     6001 taaactcggg acctacagca tgcgccccca gaaaagttgt ttttaccctg attccagcca
     6061 cttcccctaa ttgcctggca accctgagca ttttcaattc attttgcaaa tcaaggccgt
     6121 agcctgactt aatttcaaca gtagtaaccc cttcattttt tagcgcaaga agtcttggca
     6181 atgattgatc aactaactct tcttcagagg catctcgtgt catttgtact gtggataata
     6241 tacccccgcc tgatttggcg atatcagcgt aactgacgcc ttgcaatttt aatcgaaact
     6301 ctgcggctct gtggcctgca taaaccagat gagtatgaca atcaattaat cctggtgtaa
     6361 ttaattggcc gtgacaatcc tttcttgact tggcactttc ctgaaaatgg gcaggtaact
     6421 ggtcctcaga accacaccac tctattctgc cttttcttat aacaatggct tgattagaaa
     6481 gctgcaaccc tgtggcatcg attgtgcttg cattgagcaa taattcatca caggcaaaca
     6541 ttaaaattcc caatgaagta cctggtgagg cggcttcaac caggcagagt ggtgagtcgt
     6601 gtcatatata tcccactctt tggatacata cgtttcatta tcaatatcta acagcagcca
     6661 aataaaaaat tctgctactg tttcgggtga tatcaaacga ttatgatctt ttaaacgcct
     6721 ataaaaattg acctgttccg gatcaagatt aatcccgttt cgggctatcg cctgcatatg
     6781 agtgtcgata attccaggca ttacgctggc aaaggccagt gaatcgcatt caatctgcca
     6841 gcatcgtgtt aacatagcca aagctgcttt cgatacacaa taagcccccc atcctttgat
     6901 aggaaaataa gcagcagctg aacctacatt caatactctg ccacccgaca atttatcata
     6961 caaaagttgt ggaagaaaaa gtgccgcgtc tatattagtg tttaatgcct ggtgccactc
     7021 ggcaaaagaa atatctttta aaagcagtgt aggctccagg gttcctgcat tgtttatcaa
     7081 accatcaatt tgaggtgtag cacttaaact ggctttgatt aactctctgc cttgaggagt
     7141 agagacatcg gcacataaat aatgaatgtt tgcagacaaa gaagcggttt cttgcagtaa
     7201 tttttccctg cgccccacta tgaacacctg tttattgcgt tttgccaaag caaacgccaa
     7261 agctcttcct atgccgctac caccaccagt aatgagatac acaatttcct catcaatata
     7321 gaaaacaata ggtgaaataa taccagacat tagacttctt gcataacctg cacgttttat
     7381 tttagaacaa gaaaaaagca actgaacggg ctaattttat caaaagtaaa tgaaaatctt
     7441 gagtatttag catttctctt ggaaactagc tctatcatta gagctcttgc ataacctgat
     7501 tattttgttt taggacaagg cgcaaacgtt ttgaggagcg gagtttaaat gtagtaaatg
     7561 agcacccaga aacgtttgca acgcggcaat aaaactaaag aaacaggtta tgcaagaggt
     7621 ctattaaaag gagaacttga tgtttgacga tttaagtaac tatcggccag ccaaccctgc
     7681 gctttggcaa ggcagaatgg acaccgctaa ccaagaacgt ttttttcaaa aaattacttt
     7741 tattgataat caaaatgaat taatgactaa agacaaaaaa accatatttc ttggatttgc
     7801 cagtgatgcc ggaattaaac gcaatttagg cagaacagga gcaaaattag gccctgatca
     7861 aattaaaacc caacttgcca aattaccatg tcataataat aaacattatg ttgatcttgg
     7921 aaacgtggta tgtgaaaacg atgaattgga attatcccaa tctcaatttg ctcaaatagt
     7981 tcatttttgc catgaaaatg gccatcagat ctgtgctttc ggcggtgggc atgaaatagc
     8041 ctgggcgcac tatcaaggat tgtcctcact ctatccgaaa ttaggggtta tcaactttga
     8101 tgctcatttt gatttacgcc cttataaaaa aggtgagttt ggcaactcgg gaactccttt
     8161 ctctcaaatt gccacctatt gcgaagaaaa aaaaatgcct tttcattatt gttgtattgg
     8221 ggtgcaaaaa tttggcaata ccccttctct ttttgaaaaa gcaaaagaat taaatgtcag
     8281 ttacctctca gcagaagacc tttatgagca aagccaggct tggcaaattg catttcttga
     8341 tgattttatc cttaatctgg accatattta cttaacgata tgcctggatg ttctcgccga
     8401 atgctatgcc cctggtgtaa gcgccccgca agcattaggt ttatctccct ggcaaataat
     8461 gcccctgttg aaatacctta tacaaagtgg caaagtagta agtttagata ttgcagaact
     8521 ttcgccgcca ctagattccg aattaaaaac tgctagacta gcggcactta ttatcgcaga
     8581 attgttagat actaattgag gagtatattt catgaagaaa actgtgctgt ggggtgcttt
     8641 atttactcta atcactactc aagcgaactc tgaagcaact cagcaagcga tatcaactca
     8701 aagttcggcg tccaccggca ctaatgtagt ttctgagtca acgaatcagg tacctgccac
     8761 gaatcaaaac acgcagcaaa atcagctgaa ccaacctgta cagtcaaatg gacaacaacc
     8821 aactcaaacc attcaaaaca atcaaccaaa tcaaccgagc cagacagggg aacaacagtc
     8881 taaccaaaac acccagaata atcagcagaa tcaaccagca cagcaacaaa ccacacagca
     8941 accagctcaa caacaagtac cttcacaaca aacagcgcca gttattaatt gtgattacaa
     9001 gatttccgcc gaaacaaaag caatagagca atccctggtt ttaacctggt cagagaaagc
     9061 aataattcag tcatttgatt ttgatccggc tactgtagat gcccaattgc aaaaattaca
     9121 agcctgcttt acagatcaag gatggctaag ctttaattca gcattgcaaa aatcaggtaa
     9181 tctcgatgcc attaaatcac aaaagttgca tgttagcagc caaattgatg gtcagccaca
     9241 agtgactgaa gcaatagaaa atcaatggaa aattatcata ccattacaag ttgtctatca
     9301 aaatgacaag gagaaagtaa cccaattact taatgtcaac gtaactgttg gacgaaaaat
     9361 taccggtgat ttaggcatca accaaatgat cgctgtaccc cgcacagcaa ccaatacctc
     9421 acaaaccaat tcaccgtcta atacaaaccc agcagacgca aatacctcga cccctcaaac
     9481 acctggtgac acaacaaata atgctaccca aaccaataca caaacacaga ctgggacacc
     9541 aaccaactca cctccgagtg gctccacaac aaatacccag aattaaattc aacaattagt
     9601 gcggcattcc attaagaatg ctgccaaaat cacggaaata tcatgcgatt aatatttaca
     9661 ggagtattat gcctgttgac tctcaatgcc caggcgaatg tgatacagta ttttgcagga
     9721 attagttata acaaccctgc tgacttattc aaagtaaaaa atggtatctt gcttattgga
     9781 ggaaccggtt cttatgctga cttgcaattt aagggaagcg ccttaaattt taatacgttt
     9841 caatacgaat caggagttaa tcactcgcgt acttatattg tttggccata tggtcgagtt
     9901 gccaagcgcc tcaatgataa gactgtagtt gcagtagatc ttacagaacc ttttaattcc
     9961 aatcttgatt ggggcaacga cgcttttaca agatatgctg cgacccaaaa ttacctgacc
    10021 gatgtggatc taagtcccaa aatatcctat gcgattagca aaaaattaca aattggtggt
    10081 ggcataaact ttaatgtact actaaagaat gaagtcaatt gggcttttcc tactggccaa
    10141 tccacttatg cgaatttaat taaccgatcc tctagttttg gtgtgggtta taatttgggg
    10201 attaattatg cagtgaatga taccaatttc cttggtatca cttattattc aagaattcgc
    10261 caaaatactt ccggtaccag ttatctggga ttagctgcca accctgattt ccaattcggt
    10321 ttttatatgc cagcaacaac ggttgccagt tatgtccata tttttaatcc caaatggctc
    10381 atcaacctac aggtttttca aagcgaatgg aacgccaatc aaaaagtgag actttataac
    10441 acagcagcac caccaccatt tactaatttt atttttgata tgcattttga tgcctcttat
    10501 gcttatcttg ccgcgatacg taaacaagtc tctgataaat taggcattgc gctggcaggc
    10561 atgatcgatg atggtcctga agaagatggc cttagaacca tcgttttccc ttctgatacc
    10621 caatattttc ttggattgat tggtgactac cgttttacgg aacatgcttc catggaactt
    10681 atcttgggac atgtgtattc taatcccagc attcaaaaca aagccaaggt aaacaatgta
    10741 cccgttccct ttactacagg ccgggtgaca attaatgcca atgttttgga tcttaaagtc
    10801 aaaatcgagg ggtgatcttc caggtgtaac cacctggcac ttcctccaag agttttattt
    10861 ttgaatagtc ctctttttaa atagccaata ataagtccat gatcctgcta tcagcccaat
    10921 cacaaaatat accggatcgg aattaaaggt agtcaaggca gtaatagatg ggcctgggca
    10981 gtatccagcc acaccccaac caattccaaa aatgatactt cccaagatta attttttctc
    11041 aatgtttttc ttctcgggta aataaaattg attacacaat attggtttat caaatttgtt
    11101 tatcagtttg taccccaaaa aagttacggt gacagcaaca gccattacaa tcagtaaact
    11161 cggatcccaa aatccattga tatcaagaaa atttaatacc ttgttaggat tgatcatatt
    11221 tgaaatagtc aacccggctc caaataacaa accacataga aatgacatga tgttatacat
    11281 taatataatc ctcccacatg tcttatcaga tacacagtaa tcattgcaga taagataaaa
    11341 agcagtgtcg ctataaagga gcgcttggaa aatctggcca gtccacatac cccgtgtccc
    11401 gaagtacaac cttggcccat tcgggttcca aaacccacca aaaaaccact aattagtaat
    11461 aggaaaaccg gataatgtgt tcttaaagga aactggataa cagggcttat gaaatagatc
    11521 aatcctgcag ctgccaaacc aagtaaaaag gcaatacgcc aggctgggaa tggtttttcg
    11581 ggtggaaaga gtccgtgtat cattccactg atacctgcta tacggccatt taaccataaa
    11641 aaaaggacag cacttaagcc aatcagtaat ccaccaatgg cacttagata aggagtaaaa
    11701 gaggttatat aatccattat ttatatttcc gtttcattta caatgaataa cacctgaatt
    11761 gagacacttt aaaaaattaa agtgtctctc aaattacatt gatgttttaa aacaatagtc
    11821 caaattttaa ttcacttttt ccaatgagga catggttttc accccgtttt gatcagggta
    11881 ggctaatacg attaccttcg ttccaactcg caagaactct tcgttcagcc atttggcagc
    11941 actggggaaa acccttacac atccatgact tgatgggtgc tctggaactt cataagccgc
    12001 gtgaatagtg aaccccctgt aaaaatacat gcaatagggc attttagccc cgcctttcgt
    12061 atcgacaggg tattcacccg aacgacagtc aatacctcgt ttgttataaa tcctgaatgt
    12121 tccggttatc gtcctgcaag attgatttgg attttcttcg caaacatcta ttccaccaga
    12181 accagctccc gtcattacac gatgaccttc ttcatcgtag gcagcccagg cagacgcttt
    12241 aggatcaaaa acaaataatc tttcgcccgg tgctttaatt tgcattggga aataattacg
    12301 tcctctttta tcccttaaat aatgaacggt cctatgtaca taacccgcat cgtcagtaat
    12361 taatgtcgac tcatcaaact caacgcacga agttaatcca aggcacatta aaataactgc
    12421 ccaataaatt tttttcattt agattttatc tccacaatag taaaacctgg tggccttaat
    12481 taatcgctta attttcgata cttaattcgt gatggtctgt tagcttcatc acccaatcga
    12541 cgctttctat ccgtttcgta ttcagtgtaa ttaccttcga aaaaggttat ttgtgaatct
    12601 ccttcaaaag ccatcaaatg agtacaaatt ctatctaaaa accatctatc gtgagaaatc
    12661 actatagcac aacctggaaa attaaggatg gcctcttcca aagctctcaa tgtctcaaca
    12721 tccaagtcat tactgggttc atcgagtagc aaaacattcc cgccactttt caataatttt
    12781 gccaaatgaa cccggttacg ctccccgcca gacaattgag aaattttctt ttgctgatcc
    12841 gtccctttaa aattaaaacg cccaacatag gctcgtgaag gcatttggaa attacctatc
    12901 tgcataatgt catggccatc tgaaatttct tgccacaccg aattgttggg gtttagattg
    12961 tcccgcatct gatcaacata agccaacttc actgtttctc caatacgaat gacgccctta
    13021 tctggttctt cctgcccagt taacattttt aaaaaagtag acttacctgc cccattggga
    13081 ccaataattc ctaaaatacc gcctttggga agtttgaaat caaaattgtc gattaatatc
    13141 cggtcaccgt aagatttgca tattttttca ccttccagaa ccaaatcacc taaccgctca
    13201 ccgggcggga tatatatttc attggtttca ttacgctttt ggaattcttt agaattcatt
    13261 tcttcaaaac gggccaagcg ggctttattt ttagcatgac gtcctttagg tgaggttcta
    13321 acccattcca actctgcttt aatagcacgt tgcaaagcat cctcttgctt tttctccata
    13381 ctcaaccgtg cttctttttg ttcaagccag gaactgtagt tccccttata cgggatacct
    13441 tcgccccgat ccaactctaa aatccactcc gcagcattat ccaggaaata ccggtcatgg
    13501 gtaattgcaa caacggtacc agaaaactct tcaagataat gttctaacca ggcaacactc
    13561 tctgcatcca aatggttggt aggttcatca agaagcaaca tatcaggact tgatagcaac
    13621 aatcgacaaa gagcaacacg tctgcgttca ccacccgaca gttttcctat tatggcatcc
    13681 cactcgggta atcttaacgc atcagcagcc acatcgagct ttctctcaag atcccaaccg
    13741 ccacaagctt ctatctcatt ttgcagctca ccttgttcca ttaacaaggt attcatttca
    13801 tcatcgctca ttggttctgc aaaacgcata ctaatcgcat cgaaacgaga cagtttctct
    13861 ttaatttcag cgacaccttc ctcaactatt tcgcggacag ttttatttaa gtcaagctca
    13921 ggttcttgag caagatatcc aattttaatt cctggttgag gtcttgcttc accttcatat
    13981 tgtgtatcaa cacccgccat aatccgcaat agagtagatt ttccagagcc atttaatcct
    14041 aatacgccaa tttttgcccc gggaaaaaaa cttaatgaaa tatcctttaa aataaacctt
    14101 tggttctcaa caatcttgct cactctattc atagtgaata tatactgcgc cataatcgct
    14161 ccaaatctct ataatcagcc ttttatcaaa caatactacc agttcagtat aactttccct
    14221 gactggcccg atgccataat ttgaaatgcg tgctgatatt catctactgg gaaattatga
    14281 gtaatgacag gagaaatatt taaaccgctc tgtaacatag caatcatctt ataccaggtt
    14341 tcaaacattt ctcgaccata aataccctta ataaccaatc ctttaaagat aacctggttc
    14401 caatctatag gggtttcttg tggtgggata cccaataaag ccacatgacc accatgattc
    14461 atcgccttca tcatatcgtt taatgccata gggttcccgg acatttccag acccacatca
    14521 aaaccctcaa gcattcccag ctcattagcc acatcaatta atttttggta tttgacatta
    14581 accgctcggc tgacacccat ttgcctggcc agttccaatc gatgatcgtt aacatcggtg
    14641 ataacaacat gccgtgcgcc aatatgtctt acgatggctg cggccattat tcctatggga
    14701 cctgcaccag taatcaaaac gtcttcgcct actacatcaa atgccaatgc acaatgagca
    14761 gcatttccaa aaggatcgag aatagccgct tgctcttctg taatgttgtc aggtagagca
    14821 atgacattgg tagcaggaag tgccagatac tcagcaaagc atcctggtcg attcaccccc
    14881 actcccagag tattacgaca taaatgacgc ttgccggcac gacaatttct gcaaaaacca
    14941 caggtaatat gaccttcacc agaaactctc tgtccaactt tcaaaccttg gacttctttt
    15001 ccaacctcta ctatttcacc ataaaattca tgccctaccg tcataggaac aggaattgtc
    15061 gcttgcgccc attcatccca ggaataaata tggatatctg taccacaaat tgcagtcctt
    15121 ttaattttta ttaaaacatc atttactccg tattcaggaa caggaatatc ctgcatccaa
    15181 attccaggct ctttttttgc tttaaccaat gatttcattg tcatacctca aattaattag
    15241 gtaataccac catttcgcaa ttcatcctaa aatatcaggt tgtgaaatgt agagtaattt
    15301 agttttagat tacagagaat tccttaccaa ccgtttcaaa cgccttcaaa gctttatcca
    15361 aatgatgcaa ttcaagagca gcagacattt gggtacgaat gcgagccaaa cctttgggta
    15421 ccactggata ggaaaacccc acaacataga cacccagatc cagcaagcga ttagcaattc
    15481 gtccggccaa actggcatct cctaacatga caggaataat aggatgttca cccgggatta
    15541 actcaaaacc taactgagtc attccttcac ggaaataatg actattgcgc tttaattttt
    15601 cagcaaggga gttatttgtg gacaatttat cgagcaccac acaagaagta tgagcaataa
    15661 cgggtgctaa tgtattggaa aacagataag gcctggatcg ttggcgcaac caatcaatga
    15721 caacttgatt tgaggcagta tatcctcctg atgcgccccc taaggcctta ccaagagtgc
    15781 cagttacgat atcgattcta tcactaactc caaaatgctc tggtgttcct ctacctgtct
    15841 tacccatgaa accaacagca tgagaatcat caaccatcac catggcgtca tacttgtcgg
    15901 ctagttcaca aattgctggc aagttagcta atataccgtc cattgaaaaa acaccatctg
    15961 ttgcgatcaa acgaaatctc gcattttttg ctgcaattaa ctgttcttcc agggccttca
    16021 tatcattatt ggcatatcga taacgtgcgg ccttacacag cctgactccg tcaataatac
    16081 tcgcatgatt cagggcatcg ctgataatgg catcttcttc cgtcaataat gtctcaaata
    16141 atcccgtatt ggcatcaaag caggaagaat acaatatagt gtcttcttga cttaaaaaat
    16201 ggctaatctt ttgttctagt tgcttatgcg gggtttgtgt tccacaaata aaacgaacag
    16261 aagccatgcc atatccgtac ttggctaacg cagcctgtcc ctccgctatt aattcaggat
    16321 cattggccaa acccaagtag ttatttgcac aaagattaat aacttctctt tcgttaacct
    16381 tcacgtcagc ttgctgttgg ctggaaataa ctcgctctga tttatacaat ccgtctgcct
    16441 tgagtgtttc aagttcattt tgtaaataat taatgaatcg ctcaagcact ataatctcct
    16501 gtatttctgg ataaatttgt aaataatacc aaattttaaa cattctcacc atgagatatt
    16561 atcaagaaaa tttcactttc aaacattaat gcttagacat aagctgaatt cttgctaaaa
    16621 ttgctccaaa gttttaacct atccaatgat tattaagacg gtagagcaaa tgaatcacag
    16681 tgcacgcata aatatgatca aacaacagtt acgaactggt gatgtattaa acgaatccat
    16741 ccttgactta tacgatgagc tcttaagaca tgagtttgtt cctgaacctt tttctcattt
    16801 tgcttattca gacatgcaaa ttcctttggc atacggtcaa agaatgttaa ctccattgga
    16861 agaaggtaca attttacaat cccttgatct taaaggtcat gaaactgtgt tggaagtggg
    16921 aacaggaacc ggttttatga ctgcattact cagtaagtta tgtaaaaaag ttatcagtat
    16981 tgactattat tctgaattta ctgccaatgc aaaacgcaaa ctcgaagagc ataattgtaa
    17041 taatgttgaa ttaattaccg gagatgcttg tcgtggatgg ttggaaagtg caccatacga
    17101 tgttatggta ttcactggtg ccatggaaaa actgacagat acccataaac tacaaatttt
    17161 acctggtggc aagttgttcg caatccttgg taaatcccct gtaatgaaag cttatctttt
    17221 tcagttggat cataatgcaa tctggacaga atccatgctt tttgaaacgg atatacctcc
    17281 tctggtagat caattgaaac cgaaagaatt tgttttctag ggaaaactcg aatgagaaaa
    17341 tctctgtttt gttggatttt aactttaggt gtttcaaccc atgtgtttgc aacagaccta
    17401 atggatatct atcaacaagc tctggagaac gataccattt ttaaagaggc atatgatacc
    17461 tatatgtcca gcacagaagc catacctcaa gctcgtgccg ctctttaccc tcaagtaggc
    17521 cttggttcac aagctggtcg caattatcag gatgctgttg ctggagcttt cagtgccaat
    17581 caatactatg gaagctattt gtggcaagtc aatgcttctc aagcactgtt taactatcag
    17641 gcatgggcca aggtagctca ggctaaagcc tctgttaaag cagcacaagc cactttcaat
    17701 gatgcagcac agaatttaat tctaagaacg gccaaagcct attttgatgt cttgtttgct
    17761 aaagacactt tagactttgc cgaagcaaaa aaacgggcga acaaaagaca atatgatcaa
    17821 gcaactcaac gttttcaagt tggcctggat gccattactt ctgtctatga agcaaaagca
    17881 gcgtatgatc aatccatagc gacagttatc gccgcccgca ataatcagat taatcagagt
    17941 gaaaatttac gaaaacttac taatcacgta tacgaaacac tcgcgccact caaagacagc
    18001 aaaattcctc ttgttaagcc agagccaaat gacgtgaatc aatggataga taccggactt
    18061 aagcaaaact ataaattata tgctgcaaaa tataatcttg aggtggcaaa agacaatgta
    18121 aaagcgatct ctgcgggcaa ctggccagtt ttttcactac agagcaacgc aagccaggta
    18181 cataataatg catccggcaa taccgttttt attccgtcaa aacaaaccca ggctaatatt
    18241 gcaatcgcga tgaattttcc tgtttttcaa ggcggcctgg tccaggctca aacccgacaa
    18301 gctcaatatg gctttcagtc tacgagtgaa aaactggaac aaacgtatcg cgatgtcatt
    18361 gtaaacagcc gcattgcttt caataccatc actgatggga tcagcaaagt aaaggcggac
    18421 agacaaacgg tcatctccgt gcaaaattct ttgcaaagta ctgaagctca atttgaagtt
    18481 ggtactcgaa ccatggtaga tgtggtcaat gctcaacaaa gattgtttga agcacaggaa
    18541 caattagccc gggatcaata cgatttgatt aattccatac ttacgctgaa ataccttgca
    18601 gggacattaa acgttaatga tctcgaacag attaactcat ggttagcaac tacacgtgtc
    18661 aatggatttt cacccgtaga taacaaaacc tccaaataac aaatttaggt ttttttatgt
    18721 cttccaatcc ttcttcagtt gaaaaaaaat tactccatta tacaggtaaa gccatagctg
    18781 attttaatat gatccagcgt ggtgacagag tcatggtatg tctgtctggc ggaaaagatt
    18841 cattcacact attaacaatt cttaaccagc tcaggatcaa atcaggaaat aaatttgaaa
    18901 tttttgcgtt taccctggat caagcccagc cagggtggaa tgatgcctgt ttgcgtcaat
    18961 ggttggctga aaaatccatt cctcatgaaa tactaacccg agacacatac agtatagtga
    19021 aggaaaaaat tcctgaaggc aaaacctact gttctttgtg ttctcgcttg agacgtggaa
    19081 tcatctacag atatgctgaa gaaaacggat ttaataaaat agcattaggt caccatcgtg
    19141 atgatcttat tcgtacattg atgatgtcaa ttctatataa tggcgatata cgttccatgc
    19201 cacccaaatt attaagtgat aataaaaagc acatagtcat ccggccactg tgctatgtgc
    19261 aggaaaaaga tattattacg tttgcatcag aacaagcctt tccaattatc ccatgcaatc
    19321 tttgtggttc tcaagaaaat ctaatgcgca aaaaagttgc ctcactcatt gatcagcttg
    19381 cgattgaaaa ccccaaagtc cccagtaaca tgcttcatgc gctgcaaagt ttgaagccaa
    19441 gtcaattgat ggatcaaaat ttttggaatt ttaaaaatct ggaagatggg cttgaaaccg
    19501 cgcagtcaat ccaatgtgaa gaggttttta atgcacagga atttgagatt gaggatgaaa
    19561 aaatttgact tatgaaaatt attatctaat atttcttttc atttttgcaa tataatgaaa
    19621 taattggtac gtaaataagc ttattatttt gacaaatgga attaaatata taaaaatagg
    19681 ctttcaaatt atcacatgac ctgtggcttt gtttcattaa cccgatactg aattcaaaat
    19741 aaaggaactt ttagattatg attgatagaa atatagttaa cctgcgtcta tttcgtatct
    19801 actctaaacg ttatttaaaa tttgattttg ttgctgcaat tgtcgttttc ctcgtggcga
    19861 ttcctttatg cttaggtatt gccctggctt ctggcgcacc tcttttttct ggtatattaa
    19921 gtggaatcat tggcggtatt gttgtaggca tatttagcgg ctcacaggtt agtgtcagcg
    19981 gacctgctgc gggtatggca gctgttgtat tagcggcaat ctctcaacta ggtgatttta
    20041 ataccttctt attagctctt acgattgcag gccttttaca aatgataata ggtgcattaa
    20101 gagcaggatt tgttgcagat tatgttcctt ccaacgtagt tcaaggctta ctgtgttcaa
    20161 tcgggatctt gctaattatt aagcaattac cacttgcatt cactctctca tcagattttg
    20221 atgagcttaa aacacattta ttggaaacaa cagaagggtt tacagtaagc cctttgttag
    20281 ctttgtcgca gcacattaat gaaggcgcac tcatcattac tactctttca ttggctattt
    20341 taatctattt tgatataacc aaaaataaaa ttctgaagga aatcccagcc cctatccttg
    20401 tagttctggc aggaatactg ttaaatgagc ttttcatctg gacaaactcc agcctggcac
    20461 aaaactcgcc tcaactggtt aatatcccag ataccaatgg ttttttccaa tttttcagcc
    20521 atcttgagta ccctgactgg tccgcctgga ctaatcctaa agtctatctc tatgcgctag
    20581 tcatatgtat cgttgcttct ttggaaacgt tgcttaatct caaagcgtca gaacgattgg
    20641 ataaaaaaag aaggcacagc ccaactaacc aggaattagt tgctcaaggc ttgggtaata
    20701 taacatcagg cttggttggc ggtattcctg ttacatcagt cattgtcaga acatccatta
    20761 atatccatgc tggttcaaaa accaaatttt cagccgtact ccatggtttt ttcatcttgt
    20821 ttgctgtcat gttaatacct ggtgcattga ataaaattcc tttgtcttct ctcgctgcaa
    20881 ttttaattta cactggctat aaactaaata aaccagccat ctatatcaat atattttcac
    20941 aaggcagcga tcgattcatc ccttttatag tgaccgtgat cagtattatt gccttcaatc
    21001 ttcttgcagg tatattaata ggtctggcta tcagtctctt ttatatatta aagtccaata
    21061 gccaagctcg tattgatatc attaaggaaa tttatccaaa tggttcaaca taccgtttga
    21121 tgcttcccca acaaatgact tttcttaaca aagctgcgtt ggtcgctgaa ttggatacat
    21181 tgcccagacg ttctcaactg attatcgatg cgcgtcattc tcaatacatt gataaagaaa
    21241 ttctggactt atttaatgaa ttgaaagaag agttggcagg cagtaaacaa atttctgtaa
    21301 atttcaccgg atttcaggaa cactataaaa ttcataacta catcgatttc attacggtta
    21361 caacctatga tgttcaaact aacctgacgc ccgcacaagt attaaacata ctgcttgagg
    21421 gaaatcagcg ctttttaagc gataaccgga ttcatcgatc aaaccaaatc gatataaaat
    21481 acactgcaaa aacacaacat ccaattgctg tggttttagc ctgtatcgat tccagagttc
    21541 ctgtagaaac catctttgat atgagtttcg gtgatctttt ttgtgtccgc atagcaggca
    21601 atgtgattaa tgacgatatt ttggccagca tagaatacgc ctgtaacgta gttggtgcaa
    21661 agcttatcat ggttctggga catacccgat gcggcgccat tcaatctgcc tgtgatggta
    21721 ttgaaaaagg tcatatcacc caattgctct ctaaaattaa gcctgcagtt aacgcagaaa
    21781 aagaaactac aacagaacgt aacggaaaaa atcaaacttt tgtaaatcat gtcactgaat
    21841 taaatgttgc gaatacctta cagaacattt ataaaaaaag tgatatttta cggactatga
    21901 tagacagtaa tgagataggg atggttggtg ctatttatga tgtaagcagc ggaaaagtga
    21961 gctgtaaaac atataccgaa gagttatcca ctttggatgg agacgaaaat aagctcctgg
    22021 ctcaaaaatt tgaaaccatt cttttagaag ccaaagcaag ttaaactttt ttgaaattgt
    22081 aaaaataccg gaaagaaata aatttctcaa gccaggcata agattttcta caagccatta
    22141 agcctgacgt atgaagttac act
//
LOCUS       DAXLDT010000047        21331 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXLDT010000047 DAXLDT010000000
VERSION     DAXLDT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21331
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.047"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..364
                     /locus_tag="ACF2SP_001368"
     CDS             <1..364
                     /locus_tag="ACF2SP_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359161.1"
                     /translation="LIATTLVLSTSLFPLISNAEDTANPNEMTKDAWLSSMTPLLPDL
                     ICKGFIQDPDLKKRFDEIKMTYEQCVTLIPESTKKCQDELYASMPDKINSETAGTWGR
                     SLGECIGKDFAEKHLIPK"
     gene            complement(571..1272)
                     /gene="dnaQ"
                     /locus_tag="ACF2SP_001369"
     CDS             complement(571..1272)
                     /gene="dnaQ"
                     /locus_tag="ACF2SP_001369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFD2359162.1"
                     /translation="MRQVILDTETTGIGPEQGHRVIEIGCIELIDRKLTGKHFHIYLN
                     PQREVDEGAFRVHGISTEFLQDKPLFQDIVTEFIQFVEGSELIIHNAPFDVGFLNSEL
                     KHVKWNKTLEDYCRILDTLILAREKHPGQRNSLDALCKRYEIDHFNRELHGALLDAEI
                     LAYVYLAMTGGQSSLFTEVESSLSQSKIKTQEIKSLSLKKPVIMKANEEELIQHQSFV
                     EFISKKSGINHWEEV"
     gene            complement(1277..1708)
                     /gene="rnhA"
                     /locus_tag="ACF2SP_001370"
     CDS             complement(1277..1708)
                     /gene="rnhA"
                     /locus_tag="ACF2SP_001370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFD2359163.1"
                     /translation="MKVEIYTDGACKGNPGPGGWGVLLRYNGREKTLHGGEAQTTNNR
                     MELMAAIKGLEALKRPCEVDLYTDSQYLQQGMKEWIKTWKRNGWRNSKKELVKNAELW
                     KSLDNLASIHNIHWHWVKGHSGHLENDLVDALANLGIEELS"
     gene            complement(1705..2514)
                     /locus_tag="ACF2SP_001371"
     CDS             complement(1705..2514)
                     /locus_tag="ACF2SP_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2359164.1"
                     /translation="MNKVIFITGGSRGIGREIALKFAAKDKANIVIAAKTAEPHPKLE
                     GTIYSVAKEIEELGGQALPVMVDVRDEQQIQNAITKTIETFGRLDVLVNNASAINLTD
                     TLNTPMRRYDLMQSVNVRATFACSQAAIPYLLKSENPHILTLSPPLNMDKTWFAPHLA
                     YTISKYGMSMCTLGLAEEFKEAGIAVNSLWPKTTIATDAIRVHFPSELYMASRKPLIM
                     ADAAYWIINQPSKSTTGNFFIDEDVLKNSGVTDFSCYAMNPKAQLCPDLFL"
     gene            complement(2526..3914)
                     /locus_tag="ACF2SP_001372"
     CDS             complement(2526..3914)
                     /locus_tag="ACF2SP_001372"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HFD2359165.1"
                     /translation="MELMTEFLALHQQHKNNPYPSLSERKKLLIAIKKILQTEAYTLA
                     EAINKDFTHRPVEETLFLEVFPTIKAINFCLKNMKKWMRKRRRSVSWLFIPAQAYVIP
                     QPLGVVGILVPWNYPVYLALVPAIYALASGNRVMIKMSELSPHIGDTLQKLVHASGIN
                     HSIRIINGGIELSKHFASLPFGHLMFTGSTSVGKLVMKAASDNLTPVTLELGGKSPAI
                     ISPTMNPAYFKRLFMGKLLNAAQTCIAPDYVLIPKGWEERVETEFKKFINTFYPDLMS
                     NEQYSSIISESHKKRLLDLVEDARSKGARVVEFGHLIPNSSKLPVFLLFDITNDMLVM
                     KEEIFGPVLPVLTYNSIFDAVDLINSFPNPLALYYFGEDKNEIKIIQTTTLSGALTIN
                     ETLMHIAIDDLPFGGVGHSGMGHYHGKEGFDTFSKLKPVLVQRFISTVSWLYPPYGAF
                     MRMFLAWVGGIK"
     gene            complement(3928..5448)
                     /gene="hutH"
                     /locus_tag="ACF2SP_001373"
     CDS             complement(3928..5448)
                     /gene="hutH"
                     /locus_tag="ACF2SP_001373"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HFD2359166.1"
                     /translation="MSEHFILQPGQLSLVSIKQILDEELSCVLAEHAFELIRASHQTV
                     KKVIDEKKTVYGINTGFGSLANQTISSDCLKELQRNIVLSHACGTGKLLPDSVVALIL
                     LLKINNLSQGYSGVRLELINALIALFNHKVYPCIPSKGSVGASGDLVPLAHLSLPLLG
                     EGEVRHQGQVISAEEGLKLAGLKPLELEAKEGLALLNGLQVSTALALSALFISETLFE
                     TAIISGSLSVDAASGSDVPFDDRIHQIRGHQAQISAASMYRNLLAGSQIRESHRHCNR
                     VQDPYSLRCQPQIMGAVLHQMQFVGQTLQVEANAISDNPLVFAEQGDILSGGNFHGEI
                     IAMAADNLALALSEIGGSAERRIALLIDKNFSGLPAFLVRESGLNSGFMIAHVTAASC
                     ASDNKALAHPHSVDSLPTSANQEDHVSMATSAARRLHEMIDNTSTILAIELLAACQGL
                     EFHKPLKTSPQLDKIYQSVRSVVKEYDKDRYFAPDIEKIKKKILDKEFSLLTLTNE"
     gene            complement(5462..7126)
                     /gene="hutU"
                     /locus_tag="ACF2SP_001374"
     CDS             complement(5462..7126)
                     /gene="hutU"
                     /locus_tag="ACF2SP_001374"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HFD2359167.1"
                     /translation="MNKDKNKRVISAPRGTEIQAKNWLTEAALRMICNNLDPNVAEDP
                     DSLIVYGGLGKAARNWECFDEIVHVLKLLNNDQTLLIQSGKPVGVFTTHEEAPRILIA
                     NSNLVPRWATWEHFNELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAAAKKHYQ
                     GDLSGRWILTAGLGGMGGAQPLAGTMAGASVLAVECDRQRIEKRLQTKYLDRYTDNLS
                     EALDWINESCRCKKPVSVAVLGNAAEIFPQLVKLGVHPSLVTDQTSAHDPLNGYLPLG
                     WTLEQAVEMRKKSPEEVVDAAKKSMAVQVHAMLEFHNRGIPVFDYGNNIRQMAFEAGE
                     KNAFSFEGFVPAYIRPLFCEGIGPFRWVALSGDPEDIYATDERVKQLIPDDPHLHHWL
                     DMAREKISFQGLPARICWVGLKDRARLALAFNEMVKNKQVKAPIVIGRDHLDSGSVAS
                     PNRETEGMLDGSDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGVVIVADG
                     TEKAAKRLARVLHNDPATGVMRHADAGYQIAKQCAKENSLWLPMES"
     gene            7349..8791
                     /locus_tag="ACF2SP_001375"
     CDS             7349..8791
                     /locus_tag="ACF2SP_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFD2359168.1"
                     /translation="MEKHPKSLRIYFTTEMWERYGFYAVQSLLALYLALHFKWPDKEI
                     YSLVGSFTALTYLSPLVGGWIADKLIGQKRAILLGAIVLFLSYCMLSLIDNSLALTSS
                     LAGVAVGTGLLKPNISSLLGNEYPVGSTKRESGFTIFYMGITTGIILGTTLPSKFSEY
                     FGWSASFTSASIGLIIAFVVFLFGIHQYKIKDYNPFVFQPGKIISAFVLLVFLWSLSF
                     YILSSPQLANIMFGLVVLFSASYILYSVNRESANQSRQTLVIGLLCIISVIFWAFYFQ
                     MFMSLTLFIARVVEPSFLGIDFPPPYYITVQSIGMLIIGYFLAKKHHSLTMIERGLST
                     GKKFVLAMVFMTLAYSIIAFVSTVVDKSILISPLLIIPAYLMISLAELLLSPVGLSAI
                     TVLADKNKVSTMMGIFFVSLGIGGFLSGKLADLTAIPTGETNIIVLKTLYATAFTQQL
                     SILFIATLGCLVLFAVIKFLLTHIQICEKQ"
     gene            8896..9234
                     /gene="rsfS"
                     /locus_tag="ACF2SP_001376"
     CDS             8896..9234
                     /gene="rsfS"
                     /locus_tag="ACF2SP_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFD2359169.1"
                     /translation="MLEKNPTLEKLLKSLEDIQAIDIKIIDVHKQTTITDFMVVTSGR
                     SSRHVKSIAQKVLEDMKVAGLPCLSSTGIEHGDWALIDFGDIILHVMQPESRHFYNLE
                     GLWEEHPGNS"
     gene            9243..9713
                     /gene="rlmH"
                     /locus_tag="ACF2SP_001377"
     CDS             9243..9713
                     /gene="rlmH"
                     /locus_tag="ACF2SP_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFD2359170.1"
                     /translation="MLKITIITLGNKMPDWVNSGVNEYAKRFHDGIQIKLIEIPLLRR
                     NKSSDLARILEKESALIKDALPANARLIALDMLGKSFSSEELALKLTQLQQISSHLCF
                     IIGGPEGLSNEILTLCDERWSLSKLTLPHPLVRIILLESLYRAWSIINNHPYHK"
     gene            complement(9976..10947)
                     /locus_tag="ACF2SP_001378"
     CDS             complement(9976..10947)
                     /locus_tag="ACF2SP_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HFD2359171.1"
                     /translation="MKFTQDKFEKFDELTKDLQIETAQNLLNRGLVNLTQTSKPHLVL
                     FKPMVDVRKLLYCVVCGEHAKVQSMLEKDCSLIFKRGKVTDCSGREFESISAFEYVLW
                     ALDKHMWAAMIACIPSNEESRGVFVQLLAQYNKVNTDGVTYRLNGNTITEQHFDFENT
                     IIKELKTQVKEMKKQVDSVNAHNPTGDAIDNWDAIDNQWREGVGGAQKLLPMHIVDEY
                     CSNEPFYPVPQFTSQPKSSKQFYNWTTKKDEDWFNLNSKMGVDLAIYKGRGDRTLGCG
                     HVGIAASRDDLGAISALCEVRTMDFIKLKSLLEERATLDNYHQVVPM"
     gene            11179..13032
                     /gene="mrdA"
                     /locus_tag="ACF2SP_001379"
     CDS             11179..13032
                     /gene="mrdA"
                     /locus_tag="ACF2SP_001379"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HFD2359172.1"
                     /translation="MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQI
                     SEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTL
                     TKLRELIPSITDEDIDNFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVSIK
                     ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGVEKYYEDILHGK
                     VGYQMVETDVSGRTLRIINKVNPHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSR
                     NGEVLTMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVRGVYPPASTIKPFVG
                     LAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQL
                     GNKMGISNIEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAI
                     GQGFMLATPLQMANATASLSQHGQRFRPHLLIKTVNSDTNETEEYKPFEEYPVSLRDE
                     ANWDIVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKYEDIPEH
                     LRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP"
     gene            13029..14147
                     /gene="rodA"
                     /locus_tag="ACF2SP_001380"
     CDS             13029..14147
                     /gene="rodA"
                     /locus_tag="ACF2SP_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HFD2359173.1"
                     /translation="MSRQTTKPVYRFTAKSLHLDFPLLGLILTLIAFGLLILYSASNA
                     NMGMIMRQSMRLLFAFLIMFVLGFIPPHKYKIWTPWIYGVGLSLLIAVMLMGKIGKGA
                     QRWLELGLFRFQPSEIMKLAVPMMAAWFFDRQSHPSSIRSIGIASLIIFIPALLIAKQ
                     PDLGTAIMVTVAGLCVVFLAGIRFKIILLIALLMCSAIPVVWNLMHDYQKQRVYTLID
                     PEQDPLGAGYHIIQSKIAIGSGGLMGKGWLKGSQSHLNFLPEHATDFIFAVSGEEFGF
                     AGGFAIVALIVLISLRSLNIANNAQTTYTRLLSASLAMTFFLSAFVNIGMVMGIIPVV
                     GIPLPLVSYGGTAMVTFLASFGILMSISSHRILFNSLN"
     gene            complement(14259..14834)
                     /gene="rnhB"
                     /locus_tag="ACF2SP_001381"
     CDS             complement(14259..14834)
                     /gene="rnhB"
                     /locus_tag="ACF2SP_001381"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFD2359174.1"
                     /translation="MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKL
                     SPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVVV
                     DGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
                     ATPVHKEALMRLGPCKIHRRSYSPVADLISK"
     gene            15055..16017
                     /locus_tag="ACF2SP_001382"
     CDS             15055..16017
                     /locus_tag="ACF2SP_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD2359175.1"
                     /translation="MNKIRCAVIGVGYLGRFHAQKYQLIPNAELVAVCDVNSTVCEAV
                     SSELNVPAYINYHDLFGKVDAVSIAATTNKHFEIAKFCLQHGIHVLIEKPITETVSQA
                     EELIRLAKQHQVKLQVGHLERFNSARLALDEYLELPLFIESQRLAPFNPRGTDVNVIL
                     DLMIHDIDIIQNIVQSPIKSITAQGAPVLTKAIDIANARISFANHCVANVTASRISFK
                     TERKTRIFQENSYISIDYQNKQLAVFKKGTGEMFPGIPDITRDQREFEKGDALLEEIR
                     AFLNCIENDSVPLVTGEEGRDALKTAEQITALINNNLVERYAEC"
     gene            16004..17158
                     /gene="lpxB"
                     /locus_tag="ACF2SP_001383"
     CDS             16004..17158
                     /gene="lpxB"
                     /locus_tag="ACF2SP_001383"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFD2359176.1"
                     /translation="MPNASRVVIVAGEESGDHHAAELVKQLKAVYPNLKISGIGGKHL
                     RAAGVHLISDLTRYAVTGLTEIIPFLKIFHKAFQDIKQHLSTQKPDLLILVDYPAFNL
                     RLAKYAKKKLGLKIIYYISPQIWAWKGKRIHLIKDSIDKMAVIFPFEKTIYENAGVPV
                     SFVGHPLVKKIASAKDKHSSRTFLGLPLDEPIIALLPGSRHSEIERHIPILVNTAKLL
                     TLDNPKLRFVVPIAGTINPDKVKAYFSNQNLTVTFIQGQAIECMSAADFVIVASGTAS
                     LECALLEKPMCIIYKSSFLTYVAAMYFIKVKFLGLCNLLANKMMVPEFLQYDCNEIEL
                     SRYISNFHSDPNQPKSMINQLAKLKESLSSSQADCSLFDLVVAELPEKNA"
     gene            17274..17570
                     /locus_tag="ACF2SP_001384"
     CDS             17274..17570
                     /locus_tag="ACF2SP_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2359177.1"
                     /translation="MNVIDTQATTQSTKQEQGLQDLVYSLVTRFLAENKSKSINDLYD
                     MILSEVEPPLLQAVMEKRRGNQLQAAKMLGISRGTIRKKLQRYFGTKYFRLTEE"
     gene            complement(17682..19553)
                     /gene="htpG"
                     /locus_tag="ACF2SP_001385"
     CDS             complement(17682..19553)
                     /gene="htpG"
                     /locus_tag="ACF2SP_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFD2359178.1"
                     /translation="MVSKQQTMGFQTEVKQMLHLVVHSLYSNKEIFLRELISNASDAL
                     DKLRFLALSNGSLFENDSDLKISIQINEKLQTITISDNGIGLSWEEAVENLGTIAKSG
                     TKEFISQLTGEQAKDSQLIGQFGVGFYSAFIVADKVTVKSRRAGLQPEDGIVWESKGD
                     GEFTIGYEKKLTRGTEITLHLKPENDEFLSDWRIRGIISKYSDHICWPILMKKLSEEG
                     KESKEFETVNKATALWTLQKSEISEEEYKQLYKHISHDYMDPLTWSHNHVEGKHEYIT
                     LLYIPAHAPFDLWQHEAKHGLKLYVKRVFIMDEATQFLPRYLRFVKGIVDASDLPLNI
                     SREILQDNKQVESIRAACTKRVLSMLEKMATNDKETYQKFWNEFGLVLKEGPIEDFAN
                     KEAIAKLLRFSTTASGSEKQEVSLEEYVSRMKEGQDKIYYITASSYNAAKNSPHLEIF
                     RKKGIEVLLLSDKVDEWLVGYMNEFAGKKLQSISKGKIELGDDETSEQIKEQEKTLEP
                     LIKHIKSVLNDRVKDVLLTNRLTDSPACVVADEQDMGLEMQRILQAAGQQVPVSKPIF
                     EINPDHALIKRLHDIQDDNQFELWVTMLFEQAVLAEGGQLDNPADFVNRVNRLLVSS"
     gene            complement(19755..>21331)
                     /locus_tag="ACF2SP_001386"
     CDS             complement(19755..>21331)
                     /locus_tag="ACF2SP_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 88 protein"
                     /protein_id="HFD2359179.1"
                     /translation="KARRDQQLSKLRMRFFSALNHTSEIDLHMLFNDLKSILTLDSIE
                     HLKEGSVAYAIIQELLKQDDAQNKIQSFLHGAIKNVIHPGVIKGLTPDEINWNVAKAY
                     PKYYEHEEFPDVTFGGFKVRDSNEFKFKTNIQTSIWFSIKPDLFMPSKQQEALKRRRE
                     QYPGCEIRLIYSSSLLNAEANRQMKAFARKQNISLIDVDSVKTDSPLYPLLKSELAHL
                     GKGGNPAAASDLCRWIPELFNEGFYVDIDLPVDSSKIVEGHQITGGVPIMLNMGSIIS
                     EPIAPHHRRQEAVCMNTDIIAYSNDKRTQKMMDTVAHHLKNIYDDPYTALKDAPLAQT
                     AFFNKCKEEKKSIFDLRKGLQDAFRSDSLLQLYDFLGADKFKEVFKLKEAQSKYITEH
                     ISEFSEKDLLLNLISDKPSEINQHTLDFVKAKAMYIDIAKEHYSAFYKPLVEEISGPG
                     AIYNALGGAGSFTTTHRRLTGPMLPTTPPRVLQVFCDAHDKGPFVSDNIARWQTNVRD
                     LGVLNREGLSWLPSVG"
BASE COUNT         6463 a         4429 c         3743 g         6696 t
ORIGIN      
        1 acttattgct accacgctcg tgctaagcac ttcattattt cccttaatat ccaatgctga
       61 ggacacagca aatcctaatg agatgactaa ggatgcttgg ttaagctcta tgaccccctt
      121 attaccagat cttatctgca aaggatttat tcaagatccg gatctcaaaa aacgatttga
      181 tgaaattaaa atgacttatg aacaatgcgt gactttaatt cctgaaagca ccaaaaaatg
      241 ccaagatgaa ctgtatgcca gcatgccaga taaaatcaat agtgaaacag ctggcacatg
      301 gggaagatca ttaggagaat gtatcggaaa agattttgct gaaaaacatc taattcccaa
      361 atagataata aaaaaattgc ttgattgaga taatcattag gcttcgcctc tgcctataac
      421 ccatcacttg gattgcttgt tttgaggata gcgaccagga gtaagatgtc taaagaaatt
      481 tgttatttta taaggtttag tattggcaga cgcgaaaccg aataataagc gtaacagaat
      541 aattatttat aatttatata atcatgagag ctatacctct tcccaatgat tgatacccga
      601 ttttttagat ataaattcaa cgaaactttg atgttgaatt aattcctctt cattagcctt
      661 cattattact ggttttttta aacttaatga ctttatctct tgagttttta tttttgattg
      721 actcaaactg gattcaacct cagtaaataa actgctttgg ccacctgtca ttgccaaata
      781 cacataagct aatatttcgg catcaagcaa agcaccgtgc agttcacgat taaaatgatc
      841 tatttcataa cgtttacaca aagcatccaa actattacgt tgtcctggat gtttttctct
      901 ggctaaaatc aaagtatcaa gaatccggca ataatcttct aatgtcttat tccatttcac
      961 atgttttaat tcagaattca gaaatccaac atcaaatgga gcattgtgaa taattaattc
     1021 tgaaccttcc acaaattgta taaattcagt tacaatatcc tggaataaag gcttatcttg
     1081 caaaaattca gtacttatac cgtgaactct aaatgcccct tcatccactt cacgctgtgg
     1141 attaaggtat atgtggaagt gcttgccggt taattttcta tcgataagct caatacagcc
     1201 tatttcaatt actctatgtc cctgttcagg tccaatacca gtcgtttcgg tatctaatat
     1261 aacttgtctc ataactctat gataattcct caatacccaa gtttgccaaa gcatcaacca
     1321 aatcattttc caaatggcct gagtgccctt taacccaatg ccaatgaata ttatgaatag
     1381 aagccagatt atctaatgat ttccataatt ccgcattttt aaccaactct ttcttggagt
     1441 tacgccaacc atttcttttc catgttttga tccattcctt cattccttgc tgcaaatatt
     1501 gggagtcggt atacaaatcc acctcacagg gtcgttttaa tgcttccagg cccttaattg
     1561 ctgccattaa ttccatgcga ttattggtag tttgcgcctc tcctccatga agagtttttt
     1621 ctctaccatt atatcggagc aatacgcccc agccgccagg ccctggattt cccttacatg
     1681 caccatcagt atagatttct accttcataa aaataaatca ggacaaagtt gagctttagg
     1741 attcatagca tagcaagaaa aatcagtgac accactattt tttaatacgt cttcatcaat
     1801 aaaaaaattt ccagtggttg attttgatgg ttggttgata atccaataag cagcatcagc
     1861 cataatgagt ggctttctgc ttgccatata cagctcactg ggaaaatgaa ctcttatggc
     1921 atcagtagct atagtagttt tcggccataa agaattaaca gcaattcccg cctctttaaa
     1981 ttcctcagcg aggcctaatg tgcacatgct cataccgtat ttactgatgg tataagccag
     2041 atgtggtgca aaccaggttt tatccatatt taaaggtggg gacaaagtca gaatatgcgg
     2101 attctcgctt tttaaaagat atggaatggc tgcttgagaa catgcaaacg ttgccctgac
     2161 attcacactc tgcatgagat cataacgcct cattggagta tttaatgtat cagtcaaatt
     2221 gattgcgctc gcgttattga ctaatacatc caatcttcca aaagtttcta tggttttagt
     2281 gatcgcattt tggatctgtt gctcatctct gacatcaacc attacaggca gggcctgtcc
     2341 gcctaactct tcaatttctt ttgcaactga ataaatagtt ccttccaatt taggatgagg
     2401 ttctgctgtt ttcgctgcaa taacgatatt agctttatct tttgctgcaa actttaaagc
     2461 gatttctcta ccaattcctc tgctgccacc cgtaataaaa atgactttat tcatgatttc
     2521 tccttctact tgatgccacc aacccaagcc aaaaacatac gcataaaagc accatagggt
     2581 gggtataacc agctcactgt agatataaag cgctgcacta ataccggttt taattttgaa
     2641 aaggtatcaa acccttcttt tccatgatag tgccccatac cactatggcc aacaccgcca
     2701 aatggcaaat catcaattgc aatatgcatt aaggtttcat taattgtcaa agcaccagat
     2761 aatgttgttg tttgtatgat ctttatttca ttcttatctt ccccaaaata atatagcgca
     2821 agcggattgg gaaaagaatt gattaaatct acggcatcaa atatcgaatt ataagtcaac
     2881 acaggaagaa cagggccaaa aatttcctcc ttcataacaa gcatatcatt tgttatatca
     2941 aataataaaa acacaggtag ctttgaacta ttcggaatta aatggccaaa ctcaacaaca
     3001 cgggcccctt tgcttcgcgc atcttcgacc agatcaagta atcgcttttt atggctctct
     3061 gatatgatac tggaatactg ctcattactc attaaatctg gataaaaggt attaataaac
     3121 ttcttaaact ctgtttcaac tctctcctcc catcctttag gaatcaatac ataatcaggt
     3181 gcaatacaag tttgagcggc attcaataat ttccccataa acaggcgctt aaaataagct
     3241 ggattcatgg tgggagatat tattgccgga gatttaccac ctagctccag tgtcactggg
     3301 gtcaggttat cgctggcagc cttcatcacc agttttccta cgctggttga accagtgaac
     3361 ataagatggc caaaaggcag agaagcaaag tgttttgata gctcaatacc gccgttaatg
     3421 attctgatag aatggtttat tccacttgca tgtaccaatt tttgtaaagt atcgccaata
     3481 tgtggagata actcggacat ttttatcatt accctgtttc ccgatgccaa ggcataaata
     3541 gcaggtacta aagccagata gacaggataa ttccagggaa ccaggatgcc tacaactcca
     3601 aggggttggg gaataacata ggcttgtgca ggaataaaca accaagacac actccttcgt
     3661 ctctttctca tccatttctt catatttttt aaacaaaaat taatggcctt tatcgttgga
     3721 aaaacttcca aaaataaggt ttcctcaaca gggcgatgag taaagtcctt atttattgct
     3781 tctgccaaag tgtaagcctc tgtctgcaag atttttttta ttgcaatcag caattttttt
     3841 ctttcagaca aagaaggata aggattgttt ttgtgttgtt gatgcaaagc tagaaattct
     3901 gtcatcaatt ccattgtatt tttcctgcta ttcattcgtg agtgtgagta atgagaattc
     3961 cttatctaaa atcttctttt taattttttc tatatcaggt gcaaaatagc gatctttatc
     4021 atactcttta accacgcttc ttacgctttg ataaatttta tccaattgcg gtgatgtctt
     4081 tagaggttta tgaaactcca gcccttgaca agctgccaat aactcaatag caagaattgt
     4141 tgaagtatta tcgatcattt catgcaatcg acgtgctgcg ctagttgcca tagaaacatg
     4201 atcttcctga tttgccgagg taggcaaact atcaacagag tgcggatgag caagcgcttt
     4261 gttatcactg gcacaggagg cagcagtaac atgggcaatc ataaaacctg aatttaagcc
     4321 gctttctcta accaggaaag caggcaatcc actaaaattc ttatcaatta ataaagcgat
     4381 tcgccgttct gcactacctc ctatctcgga taatgccaat gctaaattgt cagcagccat
     4441 tgcgatgatt tcaccatgaa aatttccccc agacaaaata tccccctgct ctgcaaaaac
     4501 gagaggatta tcggaaattg cattggcctc tacttggaga gtttgtccaa caaactgcat
     4561 ctgatgaaga actgcaccca tgatttgtgg ctgacatctt aaagaataag ggtcctgaac
     4621 acgattacaa tgcctgtgtg actctcgaat ttgactacct gccaataaat ttctgtacat
     4681 gctggcagca gaaatttgag cttgatgtcc tcgaatttga tgtattctgt catcaaaggg
     4741 cacatcactc ccactggcag catctactga caaactgccg gaaataatcg ctgtttcgaa
     4801 caatgtctca ctaataaaca gagctgacaa agctaaagcg gtagatacct gtaagccatt
     4861 aagcagtgct aatccttctt tagcttccaa ttcaagcggt ttcaatccag ccagttttag
     4921 cccttcctca gcactgatca cctgaccttg atgccttact tcgccttctc ctaacaaagg
     4981 taaagacaaa tgcgcaagcg ggactaaatc tccggacgca cctaccgaac ccttagaggg
     5041 aatacatgga tataccttat gattaaacaa agcaatcaat gcattgatta attccagcct
     5101 aactccggaa tacccttgag acaaattatt aattttcaaa agcaaaatca aggcaaccac
     5161 gctatccggc aataatttac ccgttccaca agcatgagaa agtacaatat tacgctgcaa
     5221 ctctttcaaa caatctgaag aaatggtctg gtttgccaga gaaccaaaac ctgtattgat
     5281 tccatatact gttttctttt catcaattac ttttttaacc gtttggtgag aagctctaat
     5341 taattcaaag gcgtgctcag caagaacgca agatagttcc tcatctaaaa tttgtttaat
     5401 agaaactagc gataactgac caggctgcaa tataaaatgt tctgacataa acctgctcct
     5461 tttaagattc catgggtaac caaagcgaat tctctttggc acattgcttg gcaatttgat
     5521 aacctgcatc agcatgcctc attacccctg tcgcaggatc attgtgtagc acgcgagcca
     5581 atcgtttagc cgctttttct gttccatcag caacgatcac aacgccagca tgttgagaaa
     5641 agcccatacc aacccctccc ccatgatgaa tgcttaccca agtagctcca ctggcacaat
     5701 ttaacagcgc attaagcaaa ggccaatcag aaacagcatc actgccatcc agcataccct
     5761 ctgtttcgcg gttggggctg gccacagaac cagaatccaa atggtcacgg ccaatcacaa
     5821 taggcgcttt aacttgttta tttttaacca tttcattgaa agccagggca aggcgcgctc
     5881 tatcctttaa ccctacccaa catatccttg caggtaatcc ttgaaatgaa attttttccc
     5941 tggccatatc taaccaatga tgtaagtgag gatcatcagg aattagctgc tttactcgct
     6001 catctgttgc ataaatatct tctggatcac ctgacaatgc aacccatcga aatggtccga
     6061 ttccttcgca aaataatggc ctgatataag caggtacaaa tccctcaaag gaaaaagcgt
     6121 ttttttctcc cgcttcaaat gccatttgtc taatgttatt gccataatca aatacaggaa
     6181 tgcctctgtt atgaaactca agcatcgcat gcacttgaac agccatcgat ttcttggctg
     6241 catcaacaac ctcttctggt gatttttttc tcatttcaac agcttgttcc aatgtccatc
     6301 ctaatggcaa atagccattg agcggatcat gagcgctggt ttgatcggta accagggaag
     6361 ggtgaactcc caacttaacc agctgaggaa atatttcggc agcatttccc aaaacagcta
     6421 ctgaaactgg ctttttacag cgacaggatt catttatcca gtctaatgct tcacttaaat
     6481 tatcagtgta ccgatccaga tacttcgtct gtaaacgttt ctcaatcctt tgcctgtcac
     6541 attctacagc cagaacgctg gcaccagcca tagttccagc taaaggttga gctccaccca
     6601 ttccccctaa acccgctgtt aaaatccaac gcccggacaa atcaccctga taatgttttt
     6661 tagctgccgc aacaaatgtc tcataagttc cttgcacaat gccttgagaa ccaatataaa
     6721 tccagctgcc ggcggtcatc tgaccataca tcatcaggcc tttcttatcc agttcgttaa
     6781 aatgctccca agtagcccag cgcggtacca aattagaatt ggcaattagt attctgggag
     6841 cctcttcatg ggtagtaaat acaccgacag gttttccgga ctgaatgagt aaagtctgat
     6901 cattatttaa taattttaat acatgaacaa tttcatcaaa acattcccag tttctggctg
     6961 ccttgcctaa tccaccataa acaatcaaag aatcgggatc ctctgcaaca ttgggatcca
     7021 aattattgca aatcatcctt aatgccgcct cagttaacca atttttagct tgaatttcgg
     7081 tacctctagg ggcagaaata acacgtttat tcttgtcctt gttcatcaag aatactcctt
     7141 attgctatga aaacttcagt tattatttcc tatcaaatag cgattaaaaa attttttagc
     7201 atcatacacc agtcaactac ctccgggaat gagataatct aatgagttga gaaaaaccag
     7261 ataaaatgac caatcaatgt tcatactgtt ttttatatga tataattttt atttaataaa
     7321 ttaatttaat cgtagttact taaataatat ggaaaaacac ccaaaatctc tccgcatcta
     7381 ctttacaacc gaaatgtggg aacgttatgg tttttacgca gttcaatcct tattagcact
     7441 ttatttggcc ttgcacttta aatggccgga caaagaaatc tattctttag taggatcgtt
     7501 cacagcactc acctacctct caccattagt aggaggctgg attgctgata aattaatagg
     7561 ccaaaaacgc gctatattgc tgggtgctat tgttctgttt ttaagttatt gcatgttgtc
     7621 attgattgat aattctctgg cattaacctc ttcacttgca ggagtagctg taggaacagg
     7681 tttattaaaa cctaacattt cttcattact aggtaatgaa tacccagttg gatcaacaaa
     7741 aagagaaagt ggttttacta ttttttatat gggcatcacg acaggaataa tcctcggaac
     7801 aacattgcca agcaagttta gtgagtattt tggctggtct gcttccttca ccagtgcatc
     7861 aatcgggcta atcatcgcat ttgtagtgtt tttatttgga atacatcaat acaaaattaa
     7921 ggattataat ccttttgttt tccaaccagg taaaatcatt tcagcttttg tgttattggt
     7981 ttttctgtgg tcattgtcat tttatatttt aagttcacct cagctggcga atatcatgtt
     8041 tggacttgta gttctttttt cagccagtta cattttatat tcagtaaatc gtgaaagtgc
     8101 aaatcaatcc aggcaaacat tagttatcgg gttattgtgc atcatctctg ttattttctg
     8161 ggcattttat tttcagatgt tcatgtcttt aacattattt attgcgcgag tagtcgagcc
     8221 aagcttttta ggtatcgatt ttccccctcc atactacatc acagtgcaaa gtattggcat
     8281 gttaataatt ggttattttc ttgccaaaaa gcaccatagt cttacaatga ttgaaagagg
     8341 cttaagtacc gggaaaaaat ttgtacttgc tatggtattc atgacactcg cctactcaat
     8401 tattgccttt gtaagtactg tagttgataa atccattttg atttcaccgc ttcttatcat
     8461 ccctgcctac ctgatgattt ctttggctga attactgctt tcaccagtag ggttgtcagc
     8521 aataactgta cttgctgaca aaaacaaggt aagcaccatg atgggtatct ttttcgtttc
     8581 attaggaata ggaggattcc tttcaggaaa attagccgat ctgactgcca ttcctactgg
     8641 agagacaaat ataatcgttt taaaaacgct ctacgctaca gcattcaccc aacaactcag
     8701 tatattattc attgctacgt taggctgcct ggtattgttt gctgtaatta aatttttatt
     8761 gactcatatt cagatatgcg aaaaacaata attagatttt tacgttttag ttttccagac
     8821 ttgaaaacta aaaccctttt tatacaaatc aattgttgat attattcaac ccttttgacg
     8881 gaatatatct tagttatgct tgaaaaaaat cctacgctcg aaaaactgtt aaaatctctt
     8941 gaagacattc aagcgataga tattaagatc attgatgtgc ataaacaaac aacaatcact
     9001 gattttatgg tcgtcacatc aggccgttca tccagacatg tcaaatcgat agctcaaaaa
     9061 gttctggaag atatgaaggt tgctggtctg ccttgcttga gttctactgg aatagaacat
     9121 ggtgattggg ctttaattga ttttggagac atcatattgc atgtcatgca accagaatcc
     9181 cgacattttt ataaccttga aggattatgg gaagaacacc caggtaactc ctgacattat
     9241 ctatgctaaa aattaccatt atcactcttg gcaataaaat gcccgactgg gttaactcag
     9301 gagttaatga atacgcaaag aggtttcatg atggcatcca gataaaactc atcgaaatcc
     9361 ctttgctgcg tcgtaataaa tcctcggatt tagcaagaat actggaaaaa gaaagtgctt
     9421 tgattaaaga tgcccttcct gccaatgctc gcctcattgc cctggacatg ctgggaaaat
     9481 cctttagcag cgaggagtta gcacttaagt tgacgcaatt acaacaaatt tcaagtcacc
     9541 tttgttttat aattggcggc ccagaaggat tatccaatga aatactgact ctttgcgatg
     9601 aacgctggtc gttatccaaa ttaacccttc ctcatccctt ggttcgcatt attttactgg
     9661 aatccctcta tagagcatgg tctatcatta acaaccatcc ttatcataaa taaggatata
     9721 tagtcatttc aaaaattcct catgcatgaa ctctaatccg aaaataacag ggtgtggtct
     9781 ccaattaata tacatttatt tctaaataac cagtaaattt ccgccaaacc aattaatcct
     9841 gcttgattaa tacgccccaa aactattccc aacgatcttt ggttgctatc aattctctta
     9901 ctttagattg atactcacat caacatatgg tgacttacac cgccaggctt cttcatagac
     9961 atgtcgtcaa ctcgatcaca ttggaacaac ttggtgataa ttatccagtg tagcgcgctc
    10021 ttcaagtagc gatttgagct taataaagtc cattgttctt acttcacata atgccgatat
    10081 agctcccaaa tcatcacgag atgccgcaat gcctacatgc ccgcacccca gggtacgatc
    10141 accgcgacct ttatatatag ctaaatcaac ccccatctta gaattcaggt taaaccaatc
    10201 ttcatccttt ttagtagtcc aattgtagaa ttgtttcgat gattttggct gtgaggtaaa
    10261 ttgaggcaca ggataaaaag gttcattaga gcagtattca tcaacaatat gcatcggcag
    10321 cagtttttgt gcaccaccaa caccttctct ccactgatta tcaatggcat cccaattatc
    10381 aatggcatct ccagtcggat tgtgggcgtt taccgaatct acttgctttt tcatctcttt
    10441 tacttgagtt ttaagctctt taatgatggt attttcgaaa tcaaaatgct gttcagttat
    10501 ggtgttccca ttaagtctat aagtgactcc gtctgtgtta actttattgt attgagcaag
    10561 caattgtacg aatactcccc tgctttcttc gttacttggt atgcacgcaa tcattgctgc
    10621 ccacatgtgt ttatccaagg cccacagtac atattcaaaa gcactgatac tctcaaattc
    10681 tcgtccagag caatctgtga ctttacctct tttgaaaatt aaactacagt ctttctctaa
    10741 cattgattgc accttagcat gctcaccaca tactacgcag tacagtaact tacgaacatc
    10801 aaccatgggt ttaaataaaa ctaaatgagg ctttgatgtc tgggtcaagt ttactaaacc
    10861 acgatttaat aaattttgtg ctgtttcgat ttggagatct ttggttaatt catcaaactt
    10921 ctcaaattta tcttgcgtaa atttcatttt cactaacttt atacatattg gtcaatcatt
    10981 atacttttat tgattgtaaa tatcaagaat tttatgaatt caagcagagc atactacgcc
    11041 gtagagtaat ctagacaata ggacattaat gcgagatcgt accgaaacaa catacagagt
    11101 aagtatttta taattggtaa aaatctgtaa tttcagttac tattcctact tttgttttgt
    11161 catacaacat tttctgcgat gtatcggaat caatcattta aaaactatcg tgctgaatcc
    11221 caacatcaac gatttagact taatctatta attgcaattc tgattatttt atctttaata
    11281 ctgattttaa gattggcctt cctacaaata tcagaattta aacgatatca gaccttatct
    11341 ttaaaaaatc aaatgagcgt gataccgatt gctcctccaa gaggggttat tttggataga
    11401 aatggagtat tgctcgctga aaacatacca gtgtatgtgc tggaaatcac tcccgaacgt
    11461 gttaaagaca tgaaacagac tttaactaaa cttcgagagt taattccttc aattacggac
    11521 gaagatattg ataacttcaa taaaaccaga ttacaaaatc gttcttttgt tcccattcct
    11581 atcaaattaa aattaagtca ggaagaagta gctacttttg ccagcaatca gtatcatttc
    11641 ccgggagtga gtattaaagc gagattgatg cgtcattacc ctcttgggga gattacagcc
    11701 cacgctttag ggtatgttgg tcgcattaac attcaggaac ttaagcaagt ggactcaact
    11761 aattaccgtg ctaccaattt tattggcaag gccggtgttg aaaaatacta tgaagatatt
    11821 ctccatggta aagtgggtta tcaaatggta gaaactgatg ttagtggccg cactttaaga
    11881 ataataaata aagtcaatcc tcattcagga gcaaaactgt atttaagcat cgacgccaga
    11941 cttcaggaag cggcatataa tgcattgaag gataaacgag gtgcggtagt agtaataaac
    12001 tctcggaatg gcgaagtact cacgatggtg agttctccaa gttttgatcc caatattttc
    12061 gtcagtggag tcagtaaagc ggattataaa gtgttatcca atgccctcca gcgccctctt
    12121 tttaatcgag cagttcgagg tgtttacccc cctgcatcaa caatcaaacc gtttgttgga
    12181 ctggctggtt tggataaagg cttcattacc acgaccactg aaatatatga ccccggtaag
    12241 tacaaactcc ctacttccag ccatgtttac cgtgattgga aaaaaacagg tcatggggtt
    12301 attaatttca aacgagccat taccgtctct tgtgacactt tcttttacca gttaggtaat
    12361 aaaatgggga tctccaacat tgaagacatg cttgtaaaat ttggcttggg acaattaaca
    12421 cacgttgacc ttcatgaaga agcaaatgga gtaataccta gcgctcgctg gaaaagacaa
    12481 actaaaggag tatcctggta tccaggagat actctcattt ctgcaatagg acaaggattt
    12541 atgctggcaa cccctttgca aatggctaat gccacagctt ccctaagcca gcatggacaa
    12601 cgattcagac ctcacctact gataaaaaca gtaaacagcg ataccaatga aacggaagaa
    12661 tacaagcctt ttgaagaata cccggtttcc ctgagagatg aagcaaactg ggatattgtg
    12721 attgatgcga tgcatagcgt actaaccagc aatgaaggaa caggttaccg ttttggcaga
    12781 aatcctccct acccggtagc aggaaaaaca ggtacagcac aagtatacag cggaagacaa
    12841 tatgaaaagg caaaatacga agacatacct gagcatttga gggataattc tttatttatt
    12901 gcatttactc cagtagaaaa accagaaatt gccattgccg tggtagttga aaatgatact
    12961 atcgcttcca ctgtagcacg taaagtactt gacacttatt atcaacttta tcctattaag
    13021 aaaacaccat gagtagacaa acgactaagc ccgtatatcg ttttacagca aagtcccttc
    13081 atctggattt cccattactt ggattaatac ttactcttat tgccttcgga ctcttgatac
    13141 tttatagcgc ttcaaatgcc aatatgggca tgatcatgcg ccaatccatg aggctgctct
    13201 tcgcttttct aatcatgttt gtactgggat ttatcccgcc acataaatat aaaatctgga
    13261 ccccatggat ttacggggtt gggttaagct tgttaattgc agtcatgcta atgggtaaaa
    13321 ttggtaaagg ggctcagcgc tggttagaac tggggttatt tcgttttcaa ccttccgaga
    13381 ttatgaaact ggctgtacct atgatggccg cctggttttt cgaccgacaa tctcatccaa
    13441 gcagcatacg ttcaattggt attgcttccc tgattatttt tattccggct cttcttattg
    13501 caaagcaacc tgatctaggt actgcaatta tggttacggt tgccggttta tgtgtcgtat
    13561 ttttggcagg aatacgtttt aaaatcatat tattaattgc attactgatg tgttcagcaa
    13621 ttcctgttgt ctggaatctg atgcatgact accagaaaca aagggtgtat accctgattg
    13681 atcctgaaca agatccactt ggtgctggct atcacattat tcaatccaaa attgccattg
    13741 gctcaggcgg actaatgggt aaaggatggt taaaaggtag ccaatcacac ctcaatttct
    13801 tgccagaaca tgctaccgat tttatttttg cggtaagtgg cgaagaattt ggatttgccg
    13861 gcggatttgc tattgttgcg ctgatagtct taatttcctt acgcagttta aatattgcca
    13921 ataatgcaca aactacatac acgcgcctgc tttcagccag tctggccatg acctttttcc
    13981 tttctgcatt tgtcaatatt ggcatggtca tggggattat tccagttgta ggtatccctc
    14041 ttccgctcgt cagttatggt ggaacagcta tggtaacttt cctggcaagc tttggaattt
    14101 taatgtcgat aagctcacac cgtatattat ttaatagctt gaattgattt aatttttttt
    14161 gctctgattt gcataaatca tattatgaca ttttaaaagc tatttcttat gtatttttat
    14221 ttgggcggta tttttccaaa ttaataccac tcagagtttt attttgaaat caaatctgca
    14281 acaggggagt aacttcttct atgtatttta caaggaccca aacgcattag cgcttcttta
    14341 tggacagggg ttgcataacc tttatgttct gcaaaaccat atcctggata tattttatca
    14401 agagctacca tttcttcatc acgcaatacc ttggcgagga tggatgccgc actaatttca
    14461 ggtatcaagc tatctccctg gacaatagcc ttgcatggta tgttcaattg aggagtaaaa
    14521 gctccatcga caacgacatt atctggctga atgggtaaag cttcaactgc ccttcgcata
    14581 gctaatagag tagcatgatg aatatttaat tgatctattt cctcaacttc tgctcgccca
    14641 taggcaaaag ctaacgcttc ttcctttatt ctaattgcta ataaatttct ttgttttgga
    14701 cttaattttt ttgagtcggt aagcccatct attggttttt ttaaaataac ggctgcggtc
    14761 acaacggcac ctgcaagtgg tcctctcccc acttcatcga ccccagccat caatattttt
    14821 agctcagtat tcatttaact gctccctgcg aaaaattagg cgtaagataa tagctattga
    14881 atgcaagtgc aagacaagaa tcgacttctt tgatttggac caatctctgg agtttgattg
    14941 gagttttaaa tgcctcaatt actgtatacc tggcatgata acataggaaa tacaattgat
    15001 ttatcactaa ggaaatgcga tcataagtac aaataattat caattgatga tgagatgaat
    15061 aaaattcgtt gtgcagttat tggtgttggt tatcttggca ggttccatgc ccaaaaatat
    15121 caactaattc ctaatgcgga gttagttgca gtttgtgatg taaactctac agtctgcgaa
    15181 gcggtgtcca gtgagttaaa tgttccggca tacatcaatt accatgacct gtttggcaaa
    15241 gtagacgctg tcagtatcgc agcgactacc aacaagcatt ttgaaattgc aaaattctgt
    15301 ttacaacatg ggatacatgt tctgattgaa aaaccgatta ctgaaacagt atcccaggct
    15361 gaggaactga ttcgacttgc aaaacaacat caggtgaagt tacaagtagg ccatttagaa
    15421 cgcttcaatt ctgctcgcct tgccttggat gaatatctgg aattaccatt atttatagaa
    15481 tcgcagagac tcgcaccttt taacccccgt ggaactgatg taaacgtgat acttgatttg
    15541 atgattcacg acatagacat tattcaaaat atagttcaga gccctattaa atccattacg
    15601 gctcaaggag caccggtatt aacaaaagca attgatatag ctaatgcacg cataagcttt
    15661 gctaaccact gcgtagcaaa tgtaacagcc agcagaatca gtttcaaaac ggaacgaaaa
    15721 acaagaatct ttcaggagaa ttcttatatt tcaattgatt accaaaacaa acagcttgcc
    15781 gtatttaaaa aaggcacggg agaaatgttc cctggaatac ctgatatcac cagagatcaa
    15841 agggagtttg aaaaaggaga tgcattacta gaggaaatca gagctttctt gaattgcata
    15901 gaaaatgata gcgtaccgct tgttacaggt gaagaaggaa gggatgcttt gaaaacagcc
    15961 gaacaaatca ccgctttaat taataataac cttgtggaac gctatgccga atgctagtcg
    16021 tgttgtcata gtggcaggcg aagaatctgg tgaccaccat gccgccgagc tggtaaagca
    16081 attaaaagca gtttatccga acctgaaaat tagtggcatt ggtggaaagc acttaagagc
    16141 cgccggggtt catttaattt cagatttaac gcgttatgct gtaaccggtt tgactgaaat
    16201 aattcctttc ctgaaaatat tccataaagc ctttcaagat atcaaacagc atctgagcac
    16261 acaaaaacct gatttgctga ttttggtaga ttaccctgct ttcaatttga gattggcaaa
    16321 gtacgcaaag aaaaaactgg ggctcaaaat tatttattat atcagccctc aaatatgggc
    16381 atggaaaggc aaacgtatcc atcttattaa agatagcatc gataaaatgg ctgtcatctt
    16441 cccatttgaa aagactatct atgaaaatgc aggtgttcct gtcagttttg taggacatcc
    16501 tttagtaaaa aagatagctt ctgccaagga taaacactcg agcagaactt tcttgggatt
    16561 acctttagat gagccaatta ttgccttgct gcctgggagt cgccattctg aaattgaacg
    16621 ccacataccg atattggtga atacagcaaa attgctgacc ctagacaacc ccaaacttcg
    16681 atttgtagtc cctatcgctg gaactatcaa tcctgataaa gtaaaagctt atttcagcaa
    16741 tcagaatctt actgttactt ttatccaggg acaggcaata gaatgcatgt ctgctgctga
    16801 ctttgtcata gtagcttctg gaactgcctc attggaatgt gccttattgg aaaaaccaat
    16861 gtgcattatt tacaaatcgt cctttcttac ttatgttgct gccatgtatt ttattaaagt
    16921 taaatttttg ggcttatgta atctgttagc caataaaatg atggtacctg aatttttgca
    16981 atacgattgc aatgaaattg aattatcaag atatatttcc aattttcata gtgatcctaa
    17041 ccaacccaag tcgatgatta atcaactggc aaagttaaag gaatctctgt cttccagcca
    17101 ggctgactgc tctctttttg atcttgtagt ggcagaattg cctgaaaaaa atgcatagtt
    17161 ttgatatgaa aatctagatt atcatttagc cttcatgtac aattaatttt gctttcattt
    17221 acatgtgtaa gcaattggac tgtatgattt aattaaataa taaggaagtt aagatgaatg
    17281 tcattgatac acaagctaca actcaatcca ctaaacaaga acaaggttta caggatttag
    17341 tttacagttt ggttacccgc tttcttgctg aaaataaatc caaatcaatt aatgatctct
    17401 atgatatgat cctgtcagaa gttgagccac ctctattaca agctgtaatg gaaaaacgtc
    17461 gtggaaatca attacaagca gccaagatgt tgggtattag tcgcggtacg atcagaaaaa
    17521 aattacaaag atatttcggt accaagtatt ttcgcttaac tgaagagtaa gggaaattta
    17581 aaaataaaaa ggctcatttt tgagcctttt tattttatgt tcagctcatc ccggataatg
    17641 acttgttctc atccgagatt gattgccagg ccaatggaat actaggagga aaccaggagt
    17701 ctgttaaccc gattcacaaa atcagcggga ttatccaatt gccctccttc tgccaacaca
    17761 gcttgttcaa acagcatagt aacccataat tcgaattggt tatcatcctg aatatcatgc
    17821 aaccgcttaa tcagggcatg atccggatta atttcaaaaa taggcttgct cactggaacc
    17881 tgttgacccg cagcttgtaa aattctttgc atttccaatc ccatgtcttg ctcatcagca
    17941 acaacacatg caggggaatc agtaagacga ttagtcagca atacatcttt aactcgatca
    18001 tttagaactg atttaatatg cttaatcaat ggctccaggg ttttttcctg ttctttaatt
    18061 tgttcggaag tttcatcatc ccccaattca atcttgcctt ttgaaattga ttgcagtttt
    18121 ttgcctgcaa actcattcat atagccaaca agccattcat cgactttatc actgagcaat
    18181 aatacctcaa ttcccttttt cctgaatatt tccaaatgag ggctattttt agcagcattg
    18241 tagctggatg ctgtaatata ataaatttta tcctgccctt ctttcatgcg actaacatat
    18301 tcctccagcg atacttcttg tttctcacta ccgcttgctg tagtagaaaa acgaagcaac
    18361 ttggcaatcg cttccttatt agcgaaatcc tcaataggtc cttcttttag cacaagtcca
    18421 aattcattcc aaaacttctg gtaggtttct ttatcattgg tagccatttt ttcaagcata
    18481 gacaaaacac gtttggtgca tgcagctcga attgactcta cttgcttatt atcctgtaat
    18541 atttctcgag aaatattaag cggtaaatca ctggcatcga caatcccttt tacaaatcgt
    18601 aaatatcggg gtaaaaattg tgtcgcttca tccataataa atacgcgctt tacataaagc
    18661 tttagtccat gtttggcttc atgctgccat aaatcaaatg gggcatgggc tggtatgtaa
    18721 agcaaagtaa tatactcgtg ttttccctca acatggttat gagaccaggt taatggatcc
    18781 atgtaatcat gagaaatatg tttatacagt tgcttatatt cttcttcact aatttcagat
    18841 ttctgtagtg tccaaagtgc cgtcgctttg tttactgttt caaattcttt tgattccttg
    18901 ccttcctctg aaagcttttt catcaaaata ggccaacaaa tatgatcaga gtatttgcta
    18961 ataataccgc gaatacgcca atcacttaaa aactcatcgt tttcaggttt taagtgcaaa
    19021 gtaatttctg taccacgggt caattttttt tcatatccta tagtaaattc accatcaccc
    19081 ttagactccc atacaattcc atcctcaggt tgtaatccag cacgacgact tttaactgtt
    19141 actttatcag caacgataaa cgcggaataa aagcccactc caaattgtcc tatgagttga
    19201 gaatcttttg cctgttcacc agttaattgg ctaataaatt cttttgttcc agattttgca
    19261 attgtcccca aattttctac ggcctcttcc caacttaagc caatcccatt gtcgctaatt
    19321 gtaatggttt gaagcttttc attaatctgg atgctgattt tcaaatcaga atcattttca
    19381 aacaatgaac cgtttgataa agctaaaaat cgtaacttgt ccaaggcatc agaagcatta
    19441 gaaattaatt ctctaagaaa aatctctttg ttgctataaa gcgaatgcac aaccaaatga
    19501 agcatttgct ttacctctgt ttgaaatccc atagtttgct gcttgctaac cattaaacaa
    19561 tctccccgtt aatagttgtg ttcgaataat taacttctca tatagggttg atctattaaa
    19621 tttcaagggg atagcgctaa aaaaggaaaa atcagcaaaa aaaactaatc accaataaag
    19681 tcttttatct attggtgatt attcatattg ctaatgcttg agacaatatt caaaatttgt
    19741 gtgttgaaat taagctaccc tactgaaggc aaccaactca aaccctctcg attcaacaca
    19801 cccagatctc gaacattggt ttgccaacgc gcaatattat ctgaaacaaa aggacccttg
    19861 tcatgcgcat cacaaaatac ctgtaaaaca cgtggtgggg ttgttggcaa cataggtcct
    19921 gttaaccgcc tatgagtggt agtgaagctg cccgctcctc ctaaagcatt ataaatggca
    19981 cctggccctg aaatctcctc aactaatggt ttgtagaatg cagaatagtg ctctttggca
    20041 atatctatgt acattgcttt agccttgaca aaatcaagtg tatgctgatt aatctcggaa
    20101 ggtttatcag aaattaaatt taacaacaga tccttctcgc taaattcact tatatgctca
    20161 gtgatgtatt tgctttgagc ttcctttaat ttaaatactt ccttaaattt gtctgcccct
    20221 aaaaagtcgt ataattgcaa taatgaatcg gagcgaaatg catcttgtaa tccttttctt
    20281 aaatcaaata tacttttctt ttcctctttg cacttattaa aaaaagccgt ttgcgcaaga
    20341 ggtgcgtctt ttaatgctgt atatggatca tcgtatatat ttttcaaatg gtgggcgacc
    20401 gtatccatca ttttttgagt tcttttatca ttggaataag caataatgtc agtattcata
    20461 caaacagctt cttgccttct atgatgtgga gctatgggtt cactgataat ggagcccata
    20521 ttgagcatga ttggaactcc tcctgtaatt tgatgtcctt ctactatttt tgaactatca
    20581 acagggagat caatatccac ataaaaccct tcattgaata actcagggat ccagcgacac
    20641 agatcggaag cagcagcagg atttcctcct tttccaaggt gtgcaagttc agattttagt
    20701 aatggatata atggtgaatc ggttttaaca ctatcgacat caattaatga aatattttgt
    20761 ttcctggcaa atgccttcat ctggcggttt gcttctgcat ttagcaatga actggaataa
    20821 attaatctaa tttcgcaacc agggtattgt tcccttcttc tttttaaagc ctcctgttgt
    20881 ttagaaggca tgaaaagatc aggcttgata ctaaaccata ttgaagtttg tatattcgtc
    20941 ttaaatttaa attcattact atcacgtact ttaaagccgc caaaagtcac atcaggaaat
    21001 tcctcatgct cataatattt cgggtatgct ttggcaacgt tccagttaat ttcatcaggg
    21061 gtaagccctt tgatgacacc cgggtgaata acattcttga tcgctccatg taaaaaagat
    21121 tgaatcttat tttgagcatc atcctgtttc aagagttctt gaataattgc atacgctaca
    21181 gagccttctt tgagatgctc aatactatcc agtgtgagaa ttgatttcaa atcattaaaa
    21241 agcatgtgta agtcaatttc agatgtgtga ttgagcgctg aaaaaaatct cattcgcaat
    21301 ttagagagtt gttgatccct tcttgctttc a
//
LOCUS       DAXLDT010000048        19965 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXLDT010000048 DAXLDT010000000
VERSION     DAXLDT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19965
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.048"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(53..346)
                     /locus_tag="ACF2SP_001387"
     CDS             complement(53..346)
                     /locus_tag="ACF2SP_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HFD2359180.1"
                     /translation="MTQYNFERWSEMAKKIQEPFQAIAELNVKTLQGLSYLKPDELAQ
                     VKKPEEMLEKQINLAVENGHKALDYMQKSFQIIEKAMLSLVQEAKKTTAEVKK"
     gene            complement(553..951)
                     /locus_tag="ACF2SP_001388"
     CDS             complement(553..951)
                     /locus_tag="ACF2SP_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HFD2359181.1"
                     /translation="MTRLIKKYKNRRLYDTEISQYITVEQLQKYVVDGIDFKIEDSET
                     GKDLTNSVLLQIIVEMEAGPTQFLSSEILRQIISLANHPMHVSFKEMMEQMFHAMDKP
                     LQNNPYQQATEVWNKQMQQMVHQWQKMFKV"
     gene            complement(1081..1827)
                     /gene="phbB"
                     /locus_tag="ACF2SP_001389"
     CDS             complement(1081..1827)
                     /gene="phbB"
                     /locus_tag="ACF2SP_001389"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HFD2359182.1"
                     /translation="MLNNNVVLITGGTGDIGTAVAKQLDSTFKHVIALDLVSYDEGEA
                     WLHDLKTQGYKNINFRHMDVTDYEQCAEVIGLIIEEFGGIDVLINNAGITRDAVFTKM
                     TKRQWDEVLRVNLDGMFNVTKQVVESMKAQESGRIVNVSSVNAQKGQFSQANYAASKA
                     GVYGFTKSLAQELMIKNITVNSISPGYVNTRLMKGIRPDILEGIINLIPAKRLAEPQE
                     IAWAIEFLISEKSRYITGANLSVNGGLHMY"
     gene            complement(2006..2746)
                     /gene="phbB"
                     /locus_tag="ACF2SP_001390"
     CDS             complement(2006..2746)
                     /gene="phbB"
                     /locus_tag="ACF2SP_001390"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HFD2359183.1"
                     /translation="MDKMIAIVTGGTGGIGSAISQRLADSYQVVACYYKDGRHEEAKK
                     WQDEQKQLGYDIDIVYGDIAQYSDCEKITSLVMERYGRIDVLVNNAGITKDCSLRKMT
                     PQQWQQVLDANLTSVFNMTRNVVPVMLERGYGRIVSISSINGRKGQFGQCNYASTKAA
                     LFGFTKSLALEVASKGITVNTVSPGYIETPMLAALKEDVLNSIISSIPVGRLGYPKEI
                     ADAVAFLASPDSGFITGANLDVNGGQYM"
     gene            3012..3410
                     /locus_tag="ACF2SP_001391"
     CDS             3012..3410
                     /locus_tag="ACF2SP_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359184.1"
                     /translation="MFNKFLVTTLSVVTLTLSPYGFAKDDSCEVQVAVSTPPVQFDQS
                     VAFNVTNDDGISKSIILQGGSSPKTIGKLICSPLPYTISATQYFTPTGLTNKHAQPVG
                     QCVLKAGNVILNGSNNSVAVVFPNDFICHH"
     gene            complement(3451..4638)
                     /locus_tag="ACF2SP_001392"
     CDS             complement(3451..4638)
                     /locus_tag="ACF2SP_001392"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HFD2359185.1"
                     /translation="MVFGFLNLSFWGYVLATFILTQITIASVTIYLHRNQTHRALTLH
                     PVVSHFFRFWLWLTTGMVTADWVAIHRKHHATTDVEGDPHSPVVEGIRKVFWQGAELY
                     REARKDRDMVAKYSHGTPTDWIERNVYSRHSSKGILLMLLLDLFFFGIPGMTVWAIQM
                     MWIPLFAAGVINGIGHYWGYRNFECPDAATNIIPWGFWIGGEELHNNHHTFASSAKFS
                     VKWWEFDIGWMYIRILSLFGLAKVKKLPPKLAMDEGKFHVDLDTVRAVISNRFQVMSY
                     YYKNVIRPILKHEKNTSIETKEDKKLFQRAGRLLRREDKLLSTKAKTRLQKLLDAREQ
                     LRVVYSYKQSLQNVWLKTATTQKELVEALQQWCRQAEESGLDVLKQFAQQLKGYVPVY
                     TRE"
     gene            4819..5049
                     /locus_tag="ACF2SP_001393"
     CDS             4819..5049
                     /locus_tag="ACF2SP_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359186.1"
                     /translation="MSDKVQKLIENIVPFIVIGVGIAIVVALLFMFFYVAIWGVIIGG
                     ILWLGALAKEYFFPSKSSQKEQGRIIEHDDKK"
     gene            5049..5873
                     /gene="mutM"
                     /locus_tag="ACF2SP_001394"
     CDS             5049..5873
                     /gene="mutM"
                     /locus_tag="ACF2SP_001394"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFD2359187.1"
                     /translation="MPELPEVETTKQGIKPHLEGRIITTAQVRNRKLRLPVPLNLNEL
                     CEGKHITAITRRGKYILLHMDKGYILIHLGMSGHLRIVSQTANPQKHDHVDLHINNGL
                     ALRFCDPRRFGLFIYIDENPYQHPLLAHLGPEPLSDDFNSEYLLRKAANKSQSIKSFI
                     MDSQIVVGIGNIYAAESLFLAKIHPNTSAKKITTEEFNSLTGHIKKILESAIEAGGTT
                     LRDFYSSDGKPGYFRFALKVYGRKNLPCLVCENKIETVVIAGRHSAFCPHCQPIIT"
     gene            5948..6568
                     /locus_tag="ACF2SP_001395"
     CDS             5948..6568
                     /locus_tag="ACF2SP_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359188.1"
                     /translation="MDIKKLKKAASDFLERYPDGFCHPEMIEMGKKHKMDKMVALAQG
                     SFARQNFKNPDEITNSMLKIVTQSSLISIFEKPKFRDFIKRTPPNDKVLIAKGLKELL
                     YGNEQEGFEELLFQFQREKLAKWTLMTVFQAYFNPEVDVFIKPTTTKNIIKELNLDIV
                     YNPTPSWEFYESYRNIINQMKTKIEKSLAPSNAAFCGFLMMSLDDR"
     gene            complement(6626..6696)
                     /locus_tag="ACF2SP_001396"
     tRNA            complement(6626..6696)
                     /locus_tag="ACF2SP_001396"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6662..6664),aa:Gly,seq:ccc)
     gene            7008..8075
                     /gene="dinB"
                     /locus_tag="ACF2SP_001397"
     CDS             7008..8075
                     /gene="dinB"
                     /locus_tag="ACF2SP_001397"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFD2359189.1"
                     /translation="MNPIRKIIHIDMDCFYAAIEMRDFPELANKPIAVGGDAKRRGVI
                     ATCNYAARQFGIRSAMPTAHALKLCRELILRPVRMDVYQKESQYIRSLLTEYTDLVEP
                     LSLDEAYLDVTESTQCQGSATWIAEEIRARIYQTRQLTASAGIAPNKSLAKIASDWHK
                     PNGQMVIRPEDVSAFVLDLPVRKLFGVGPKMEEKLGALNIKTCADLQRYSVEYLLQKF
                     GTMGQRLYELARGIDNRPVNPERIRKSISVEETYPKDLPNSEACLAVLPDLMARLEAR
                     IQRAGKISGIHNLFVKLKFNDFQQTTIERVMDKLDLIVLRQLIQEGFARRGMPVRLLG
                     IGIKLKQENTYQSIQLPLLDL"
     regulatory      8327..8390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            8414..8734
                     /gene="sugE"
                     /locus_tag="ACF2SP_001398"
     CDS             8414..8734
                     /gene="sugE"
                     /locus_tag="ACF2SP_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFD2359190.1"
                     /translation="MLLPWLYLIIAGLLEICWAVGLKYTDGFSKWQPSLFTVTTLIIS
                     MFLLAKAAQTLPIGTAYAVWVGIGSLGTAIMGILLFQEHLSVFRLLYLVLMLISIFGL
                     KYTA"
     gene            8803..9534
                     /locus_tag="ACF2SP_001399"
     CDS             8803..9534
                     /locus_tag="ACF2SP_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFD2359191.1"
                     /translation="MKISRLKTFWIITRLFYYTGVACTRSIFKYLFSTPTRPWVDKVI
                     HHWVDQLLNAVKVEYKVINPHNVQPQPGKATILMCNHSSAYDIPLGFKAFPNNSIRML
                     AKRELSKIPLLGKGMAAAEFPFIDRKNRFQAIKDLEFAQQLMESGIILWIAPEGTRSK
                     DGKLAPFKKGGFITAIQAKATIIPIGIRGAFDILPARTFNIHLNQKAEIHIGEPIDAA
                     QYTIENKEELIEKTHQSIKKLVGEN"
     gene            9743..11959
                     /locus_tag="ACF2SP_001400"
     CDS             9743..11959
                     /locus_tag="ACF2SP_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2359192.1"
                     /translation="MSINKIEEDRDTSSDLEMKTKMYLVHRMDKAGPLTVKDVHILIN
                     KLSKDLQKNQLTLIDCQLIINLVESRFTLVKQATRHLQFFLNKLDGKSINPILIPLSN
                     KPFWQNEPHPFDHYRSTDQLPEKADIVIIGAGLTGASTAYHLIDAVKKRNLTVVLLDK
                     GNPATEASGRNGGNFELLPENSVGIYSGLVSERFRFLKRCYPHVHPSILQIESEYHAS
                     LVFQFALKNRNRFKEIARKERIYCDLAAKGWLYIAHTEEEEQAICDEVTLAAQHGERI
                     YIWSRKKIREQFGINSKFIGRFIPDDGTYNPFKYTCCLIKAAIKSGIKLYTFVKVQQI
                     KSMDTEYHQLKTNMGLLKARQVIVATNAFTSELLPELSAIKPHQSQIAITESTPDYCK
                     GRLITSEDGPLYLNQPRVRSKNGLVPLLIGAGDDRPMKNPYYRKRSKKVHDLIVQQRD
                     KYFPDLKGKPFSTEWVGALAFTPDQLPAIGYLSPGIIIVMCCNGYGGSYTTAAGFAAA
                     EIALTGKNPPWLPADVFSPKRLLSDKPLFLEDSDSLWRIGKTLCTTLNNLNQLLAESL
                     SYQSMYQPYSTISSLPHGHQEKTCNLDPLRVMHQLAIFRTFAYQELEMLIRYTSPVCL
                     NKGEILFKQGDAGHSCFILIQGKINLYYEHAEGFTSMVAELEAGSVFGQMALFLENKR
                     MATCQATENCSMLEIMVEPCKSLFIQQQALGIKLLRLLNQGVIDALHKLNKRLKYT"
     gene            complement(12057..13781)
                     /gene="ggt"
                     /locus_tag="ACF2SP_001401"
     CDS             complement(12057..13781)
                     /gene="ggt"
                     /locus_tag="ACF2SP_001401"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFD2359193.1"
                     /translation="MKYNYKWLSCLFFLICNIYQVNAKTFPQHPPGYAVASAHPLATH
                     AGLEILAAGGNAFDAAVAVAAALAVVEPYHSGLGGGGFWLLHQEKEHLNLFIDGREVA
                     PLAASKDMYLAPDGQVIPGLSLNGGLSAAIPGQPAALVYIAKNYGRLPLSKTLAPAIK
                     LAEEGFPVDPQFRYFSTLDDRLENLKKYPDTAAIFLKNDKPFEIGEKLVQKDLAKTLR
                     LIAKYGNAGFYSGETASLLVQGVNAAGGIWSLDDLAQYKIKVRPPLISAYHNMLIITA
                     PLPSAGGVALVTMLNILSKFPLSSFPKLKWVHYVVESMRLAYWQRDQFLGDPDFIKIP
                     LDKLISSDNAKQLSRLISPDKAIDSKVLQGSVPIGKHSNTSHISIIDTEGNRVAATMT
                     INYIFGSSVVAKGTGVLLNDEMDDFSTKVGEQNVFGLVGSERNAIEPRKRPLSSMAPT
                     FLEMPGRVAILGTPGGSRIPTMVLLASLLFYDGYGAISMVSAMRFHHQYLPDVLQFEP
                     DTFPSSIQQGLQAMGYHLMPLKQYYGDMQAITWDKESNVLTAASDPRHIGLAAAISNM
                     AYGYGVNY"
     gene            complement(13771..14277)
                     /gene="coaD"
                     /locus_tag="ACF2SP_001402"
     CDS             complement(13771..14277)
                     /gene="coaD"
                     /locus_tag="ACF2SP_001402"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFD2359194.1"
                     /translation="MQMVINEMKQKAIYPGTFDPVTNGHIDIITRASTIFPELIVAVA
                     SNKNKRPYLSWETRISLLEESVGHLTGVRVVGFDNLLIDFVLEQSAGIILRGLRAVSD
                     FEYEFQLAGMNRKLSKKVETLFLTPAEHLMYISSTLVREIAALNGDISQFVPPNVVRE
                     LKKRQNEI"
     gene            14340..14939
                     /gene="lolB"
                     /locus_tag="ACF2SP_001403"
     CDS             14340..14939
                     /gene="lolB"
                     /locus_tag="ACF2SP_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFD2359195.1"
                     /translation="MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETAT
                     VSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIRLMGPLGGGTVLISKKGNTITFQ
                     DGSKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGVQSEKHDQFNHLIQLKQN
                     GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV"
     gene            15067..15141
                     /locus_tag="ACF2SP_001404"
     tRNA            15067..15141
                     /locus_tag="ACF2SP_001404"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15099..15101,aa:Gln,seq:ttg)
     gene            15321..16268
                     /locus_tag="ACF2SP_001405"
     CDS             15321..16268
                     /locus_tag="ACF2SP_001405"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFD2359196.1"
                     /translation="MSTMMIFTGNANPELALKISSHLQIPIGKATVGTFSDGETMVEI
                     LENVRGKDVFVLQSTCAPANNNLMELLIMADALRRSSAGRITAVVPYFGYARQDRRVR
                     SARVPITAKVVADMMASVGICRVLTVDLHADQIQGFFYMPVDNVYSTPVLLEDITKQK
                     LNNIMIVSPDVGGVVRARAVAKRLNDAELSIIDKRRSGPNKSEVMHIIGEPANKNCII
                     VDDIVDTAGTLCTAAQELKKNGAKSVRAYITHPVLSGPAVNNIKHSGLDEVVVTDTIP
                     LSAEAQNCEKIRVVSLADMLAQAIKRVNVEESVSSMFAE"
     gene            complement(16330..16611)
                     /locus_tag="ACF2SP_001406"
     CDS             complement(16330..16611)
                     /locus_tag="ACF2SP_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2359197.1"
                     /translation="MTTISNDAGDTTASLAESVTQSVQKYFSELKGTDPVDLYQFVLE
                     EIETPLFRAVMEHCKYNQSRAAIMLGISRGTLRTKLRRYFDDKYVGTRD"
     gene            complement(16801..18258)
                     /locus_tag="ACF2SP_001407"
     CDS             complement(16801..18258)
                     /locus_tag="ACF2SP_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFD2359198.1"
                     /translation="MKKLTGLVIILAVLILGGYYGMGILTERTVKKTVEVINQSNGLF
                     ADIQQYKRGWFCSDALVKWRLHVPERVVKDDDGKLQTTPAQDYQMEMPIKIYHGPIIY
                     ANKQLRFGMGFAQTVFPFPEKYNQQFNELFSQDSEKPQLDLSIFVNYLNKSTVELSIP
                     SFKLTAKDGNGKFNWMGMDSTTSMSSNLDKVEGEIEFDGAEFSKDDTKLSLGKVISEY
                     DLHKTTTGLYLGQASFSLPSLAVVVKDQKMFEISELTLSSSSDIDDNLFSTHFSAALK
                     SLFANGQNYGPGEVEMSLRNLDAEVLAKINEQANAMQRGTDQERQQALMAMLPELPKL
                     FSKGAEFEISKLSLKLPQGMIDGNLLITLPKGDNSNPFELIQKTQGNAKLKMPIEVVK
                     QLMQQSIMQQMAKQPEMQQALIQQLQSSQGQSNQAEPTPEQIAAMQVDKQINELEQSG
                     LITVDGKDYVIEMSLSEGKFTVNGKPFDPSMMKFQ"
     gene            complement(18486..19769)
                     /locus_tag="ACF2SP_001408"
     CDS             complement(18486..19769)
                     /locus_tag="ACF2SP_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359199.1"
                     /translation="MGISKVEIYKRKNYLGFAWLVWGLAAAFYFSDYLARVAPGVMHR
                     YLQMDFGINEAGFGILTASFYVPYILMQIPVGLTVDRLSIRWLLTIMSLVTAFGCCVF
                     GLADGLLTASIGRMLIGFSAAFAFICSLRLATSWFPPTMLGLLSGLTQALGMLGAAAG
                     EAPVSFLVSNVGWRHSMLIIAFLFIALSGLLYQFVQDKPGEHRNEIRSVNRISILDSL
                     KIILSNKQTWLNALYAGFLFGPTAVIGEAIGPAYLQFGRGLGAHAAAFATGLIFIGWG
                     ISGPLSGWISDKMGRRKPLMIISAVCGVILSSLFVFIPEMSQTTAYILFFVFGLTNTG
                     VAISYAVSTEIHDRSVVGTSIAFTNMTSIFVGALFQPLVGRIIDIVSGPRAYNVETLL
                     LSDFQAGLKLLPLCSLVALILAFTVKETYCKPIRH"
BASE COUNT         6337 a         4051 c         3557 g         6020 t
ORIGIN      
        1 tgatatgaaa atagaaaagg cttattttga tcaaataagc cttttaaagg aattatttct
       61 ttacttcagc agttgttttt ttagcttctt gcaccaaact taacatggct ttttcaataa
      121 tttgaaatga tttttgcata taatccaaag ccttgtggcc attttccact gccaaattaa
      181 tttgtttttc cagcatttct tcgggctttt taacttgagc cagttcatca ggcttcaaat
      241 aacttaaccc ttgcagggtt ttaacattca gttcagcaat ggcttggaaa ggctcttgta
      301 ttttttttgc catttcactc catctttcaa aattgtattg ggtcataata ttctccattt
      361 gttgcactgc acaatataat agtagacctt atctttatta tttgtcaata tattatctaa
      421 taattttttg cagtgcacca aaaatcacga ttacattcat tgatttttga tgtgtattgc
      481 gctttccaaa tcatcagtaa aattcgtaaa gcgaaacatt ctatttacta caaaaaagtt
      541 tgggaggaat ttttagactt taaacatttt ttgccattga tgtaccattt gttgcatttg
      601 cttattccag acttcagtcg cttgctggta aggattattt tgtaaaggtt tgtccattgc
      661 atgaaacatt tgctccatca tttccttaaa ggaaacatgc attggatgat tagctaatga
      721 aatgatttga cgtaaaattt ctgaagacaa aaattgcgtt ggtcccgcct ccatttccac
      781 tataatttgc aacagaaccg aattagttaa atcctttcct gtctctgaat cttctatctt
      841 gaaatcaata ccatccacaa catacttttg tagttgctca acagtaatat attgactaat
      901 ttcagtatca tacagtcttc gatttttgta tttttttatg agtcgagtca ttattttctc
      961 aagtatcaga tatagaaata taattataaa atgatgtgca aaattggtat agtttgcttc
     1021 tttaagccca agagtaaatc tttcacactt cttttacaaa ttctttcaag gttaaaaaag
     1081 ttaatacata tgcaaaccac cattgacact aagatttgct cccgtgatat atcgactttt
     1141 ctcactaatc agaaattcaa tagcccaagc aatttcttgt ggctctgcca atctttttgc
     1201 gggtatcaaa ttaataatcc cctccagaat atctggccta attcctttca tcagacgagt
     1261 attaacgtaa ccaggagaaa tactgtttac tgtaatattt ttaatcatta gttcctgggc
     1321 aagacttttg gtgaaaccat atactccagc ctttgaagca gcataattgg cttgggaaaa
     1381 ttgccctttc tgggcattaa ccgaagacac attcactatt cggcctgact cctgagcttt
     1441 catactctct actacttgtt tagttacatt aaacattcca tcaagattga cacgcaacac
     1501 ttcatcccat tgccttttgg tcattttggt aaacacggca tctcgagtaa ttccagcatt
     1561 attaatcagc acatcaatac cgccaaactc ttcaataatt aagcctatta cttctgcaca
     1621 ttgttcataa tcagttacat ccatatgcct gaaattaata tttttatagc cttgtgtctt
     1681 caaatcatgc aaccatgctt caccctcatc ataggaaacc aaatccaaag caatgacatg
     1741 tttaaaagtt gaatccagtt gttttgcaac agccgtacca atgtcaccag ttccaccagt
     1801 aattaatacc acattattat tcagcatgaa tttctctatt agttttttcc tgtccccaat
     1861 tgaagattta aaattggagc ttatatttta ttcattgtta acggtgatcg cagccacttt
     1921 ttcaaaaccc caaggtgtct tgcgaacagt aaataatatg ttcacaaaac ctcttgagat
     1981 tcttaatcgg tgaatcaaac tctgactaca tatactgacc accattgacg tccagatttg
     2041 cccccgtaat aaatccgcta tctggtgatg ctaaaaatgc aacggcatct gcaatttctt
     2101 ttgggtatcc caatcgacca acaggaatac tggatatgat tgaattgagt acatcttctt
     2161 tcaatgcagc cagcatagga gtttcaatat acccaggcga cactgtgttt accgtaatac
     2221 ctttacttgc tacttctaat gcaagactct tggtaaaacc aaataaagcc gccttggtgg
     2281 aagcataatt gcattggcca aactgccctt ttcttccatt gattgaagat atactgacga
     2341 tacggccata acctctttcc aacatcactg gaacgacatt gcgcgtcata ttgaatacac
     2401 tggtcaagtt ggcgtctaaa acctgttgcc attgttgagg tgtcattttt cttaaactgc
     2461 agtctttagt aatgcctgcg ttgttaacca aaacatctat acggccataa cgctccatca
     2521 ccagggatgt gattttttcg caatcactat attgagctat atctccatat acgatatcaa
     2581 tatcataacc aagttgcttt tgctcatctt gccatttttt tgcttcttca tgtcttccat
     2641 ctttatagta gcatgccaca acttggtaac tatcagctaa gcgctgacta atagcagaac
     2701 caataccgcc tgttccccca gttacaatcg ctatcatttt atccatcgac aactcctttt
     2761 gttgctaggt aacattacat tactacaatc taatttcact ttcttgtcaa tgacatatat
     2821 ataatactaa gttggtgata ataattcagc tactttattt ttgatcatgt tacggagctg
     2881 atataaatgt attaatgatg actaatttgc attacaaatc gaatgaagat accctaaatc
     2941 tgcttttaag taagcatcgt aatataagtg ttttttaata ttagaagtag cattcagaga
     3001 atgagggtat tatgtttaat aaatttttag ttacaacact atccgtagtc acattgacac
     3061 tttcaccata tggttttgcc aaagatgatt catgcgaagt ccaagtggct gtttccactc
     3121 ccccagttca atttgatcaa agtgtggcgt ttaacgtaac gaatgatgat gggattagta
     3181 aatccattat tttacaaggc ggaagcagtc caaaaactat agggaaatta atttgttctc
     3241 cactacctta taccattagc gccacccaat attttacacc aacaggctta accaataagc
     3301 atgctcaacc tgttggccaa tgcgtattaa aagctggaaa tgtcatattg aatggttcta
     3361 acaatagcgt tgctgttgtt ttccccaatg attttatatg ccaccactaa aatattagct
     3421 gcattgtgca ttgctcggga gtgcaaaaca tcactccctg gtatataccg gtacgtagcc
     3481 tttcaattgt tgggcaaact gtttcagcac atcaagaccc gattcctctg cctggcggca
     3541 ccattgctgc agagcttcta ctaattcttt ttgagtcgtt gctgttttca gccaaacatt
     3601 ttgtaatgat tgcttgtaag agtaaaccac tctcaactgt tcgcgggcat cgagcagctt
     3661 ttgcaaacga gttttagctt ttgtcgataa tagcttatcc tctcgacgca gtaatctacc
     3721 tgcccgttga aacaatttct tgtcttcttt tgtctcaatg ctggtgtttt tttcatgctt
     3781 caaaataggt ctgatgacat tcttatagta ataagacatc acttgaaagc gatttgaaat
     3841 aactgctctc acagtatcca gatcaacatg gaattttcct tcatccatag ccaatttggg
     3901 aggtaatttt tttaccttgg ccaaaccgaa aagggaaaga atgcgaatat acatccaacc
     3961 aatatcaaac tcccaccatt tcactgaaaa tttggctgag gaagcaaaag tgtgatgatt
     4021 attatgtaac tcttctccac caatccaaaa tccccaggga atgatattag tagcagcatc
     4081 gggacattca aaattcctgt atccccaata atggccgata ccattgatga ctcccgcagc
     4141 aaacagcgga atccacatca tttggattgc ccatacagtc attccaggaa tcccgaagaa
     4201 aaataaatcc agtaataaca tcagcaaaat acctttactt gaatggcgcg agtagacatt
     4261 acgctctatc caatcggttg gtgtgccatg agaatatttg gcaaccatat ccctgtcctt
     4321 gcgtgcctct ctgtaaagct cagcgccttg ccaaaatacc ttcctgattc cctcaaccac
     4381 tgggctatga gggtctccct caacatcagt tgttgcatgg tgcttgcgat ggatagcaac
     4441 ccaatccgca gtaaccatac ccgtagttaa ccataaccaa aaacggaaaa aatgactgac
     4501 cacagggtgc aatgttagcg ctctatgagt ctggttccga tgaagataga tagtaacaga
     4561 tgcaattgtg atttgagtca ggataaaagt tgcgagaaca tatccccaga aagacaggtt
     4621 taaaaagccg aataccataa taaccccaca ttgtacggaa ttataaaaat aactataatt
     4681 taagagtgaa tgtattttat tcctaaatta tagaatcaat atatgtgtaa tgataacaca
     4741 ttttttatca tttcattgca tgatttcatg atgtagtcga taataaattt actcatctta
     4801 ttttatcagg atatagacat gagcgataaa gttcagaaat tgattgaaaa tattgtgcct
     4861 tttatagtga ttggtgttgg gatcgcaata gttgtagcct tattgttcat gtttttttat
     4921 gtggccattt ggggcgtaat catcggtgga atactctggc ttggggcctt agctaaagaa
     4981 tatttcttcc ccagcaaatc atcccagaaa gaacaaggaa gaattataga acatgatgat
     5041 aaaaaataat gcctgaatta cccgaagtcg aaacaacaaa gcaaggtatt aaaccccatc
     5101 ttgaaggccg tataattacc acggcgcaag tgcgtaatcg aaaacttaga ttaccagtac
     5161 ctctaaacct gaatgaatta tgcgaaggaa agcatataac agctattacc cgtagaggaa
     5221 aatacatctt acttcatatg gataaaggtt atattctcat ccatcttgga atgtccggac
     5281 atttgcgtat cgtctcccag acagcaaatc ctcaaaaaca tgatcatgtt gatctgcaca
     5341 ttaataatgg gcttgctctc agattctgcg atccaagaag atttggctta tttatttaca
     5401 ttgatgaaaa tccttaccaa cacccactgc tggctcattt aggcccggaa cctctttctg
     5461 atgactttaa tagcgagtac ttattacgta aagctgcgaa taaatcgcaa tccatcaagt
     5521 catttattat ggatagtcaa attgttgtcg ggataggtaa tatttatgcg gcagagagtc
     5581 tttttcttgc caaaatccat ccaaatacct ctgcaaaaaa gataactaca gaagaattca
     5641 attctctgac tggtcacatc aaaaagatac ttgaatctgc catagaggca ggagggacga
     5701 cacttcgtga tttctattct tctgatggta aaccagggta ctttcgtttt gctttaaagg
     5761 tttatggcag gaaaaatctc ccctgtttgg tatgtgaaaa taaaatagaa actgtcgtga
     5821 ttgccggtcg ccactccgca ttttgcccgc attgccagcc aatcattaca taataagttt
     5881 aataatggct acctggaacc atttttgttt cttaattgat tctgttgttg tgacaaaagg
     5941 ttaatttatg gatattaaaa aacttaaaaa ggcagcctct gattttctgg aaagatatcc
     6001 agacgggttc tgccaccctg aaatgattga aatgggtaaa aaacataaaa tggataagat
     6061 ggttgctttg gctcaaggaa gttttgccag gcaaaatttt aaaaaccctg atgaaataac
     6121 aaattccatg ttgaaaattg ttactcagtc atctctgatt tcaatatttg aaaaacctaa
     6181 atttagagat tttattaaaa gaactcctcc aaacgataaa gtacttattg caaaaggatt
     6241 aaaagaactt ttgtatggaa atgaacagga aggctttgaa gagcttcttt ttcaatttca
     6301 aagagagaaa ctggcgaagt ggactttaat gactgttttt caggcttact tcaatccgga
     6361 agtggatgtg tttataaaac ctacaacaac caaaaatatc attaaagaac tgaaccttga
     6421 tatcgtatat aacccaaccc cttcctggga gttctatgaa tcttatagga atatcattaa
     6481 ccagatgaaa acaaaaattg aaaaatcact agcgcccagc aatgcggcat tctgtggttt
     6541 tttaatgatg agcctggatg atcgttaaat tttctatgag tatcgcttga tgggctctat
     6601 gaagacatgc cacattcttt tattgagcgg gtgatgggaa tcgaacccac gccatcagct
     6661 tgggaagctg aggttctgcc attgaactac acccgcaaaa actcacgaac cctttcgtca
     6721 atagaacatt aaggtatcat tttataatta ttgattcaat taagttatca caacggcgat
     6781 ttaaatttaa aaacaggttt taaaactcaa ctattaactt aaactatgca aatgtatggc
     6841 aagcataact cacagaacat agcttggttg cttcttttga ttgcaatctt atattgatat
     6901 ctaaataaag taaaaaatag ttattctgta gactaaactt tacagcttca agaaaatcag
     6961 ctaagaccta atatgaaatc tctgttattg caatctgtga ttgaaaaatg aatccaataa
     7021 gaaaaattat ccatattgat atggactgtt tttatgcggc aattgaaatg cgtgatttcc
     7081 ctgaactggc aaataaacct atcgctgtag gcggggatgc aaaacgtcgt ggagttatag
     7141 cgacctgtaa ttatgctgcc cgccaatttg gcatacgctc tgcaatgcca acagcccatg
     7201 cgctcaaact ctgccgagag ttaatcttgc ggccagtacg tatggatgta taccaaaagg
     7261 aaagccaata tattagatcc ctattaacag aatatacgga cctcgttgaa cctttatcat
     7321 tagatgaagc ctatctggat gtcactgaat ccacacaatg ccagggaagc gccacctgga
     7381 ttgctgaaga aatacgcgcc cgaatttatc aaacaagaca actcactgca agtgcgggca
     7441 ttgcgccaaa taagagtctc gcaaaaatag ccagtgattg gcataaaccg aatgggcaaa
     7501 tggttattag acccgaggac gttagcgcat ttgttcttga tttaccagta cgcaagttat
     7561 ttggtgtagg gcccaagatg gaggaaaaat tgggtgcgct caacattaaa acctgtgctg
     7621 atttgcagcg atattccgtt gaatatttat tacaaaaatt tggcaccatg ggtcaaagac
     7681 tttatgaatt agcgcgaggc attgataatc gccccgtcaa tcctgagcgt attcgaaaat
     7741 ccatcagcgt tgaagaaacc tatccaaaag acttgccaaa tagtgaagct tgcctggcag
     7801 tcttgccaga tcttatggcg cgccttgaag caagaataca acgtgcaggt aaaatttctg
     7861 gcattcacaa tttatttgta aaattgaagt ttaatgactt tcagcaaaca actattgaac
     7921 gagttatgga caaacttgat ttaatcgttt tacgtcaatt gatacaagaa ggttttgcca
     7981 gaagaggcat gccagtacga ttattaggta taggaattaa attgaaacaa gaaaatacgt
     8041 atcaaagcat acaattgcca ttacttgact tataacaagt atctaaacag ttgatatcag
     8101 attctttccc aaacttgcca taattatcag aacttaatac atcagctctc ttttttatgc
     8161 aacaccctgc aatagttagc aaaatcagga cttttatatt gaaaatatca atgcaatgga
     8221 agtgcaaaac tcctgccata ttattaatga gctattcttg actgagctat aatctatacc
     8281 atatcgctca atttgactca gggaatacta tttccttgta ggcagtgata tgctataaaa
     8341 taatctccgg acgagacgcg ccgtacccgg ttaagacaag acgacgcctt taatcaatct
     8401 aaaaggagtc gttatgctac taccctggct ttatttaatt atcgcagggc tcttggaaat
     8461 ctgttgggct gtcggtttaa aatataccga tggtttttcg aaatggcagc caagcctgtt
     8521 tacagtcacc acactgatta tcagtatgtt tttattagcc aaggccgccc aaacattacc
     8581 tatcggcact gcctatgcgg tatgggtagg gataggttca ctggggactg caatcatggg
     8641 aatactgctg tttcaggaac atctatctgt cttccgattg ctataccttg tattaatgct
     8701 gatatctatc tttggtttaa aatacacggc ttgatttgaa tcgttccagt attcctcaac
     8761 ttgttgtatg atggccctct cataatccca tgcgctctaa cgatgaaaat aagtcgatta
     8821 aaaacttttt ggataattac ccgcctattt tattacacag gtgttgcttg cactcgctcc
     8881 atatttaagt atctttttag cacccctacg aggccatggg ttgataaagt catccaccat
     8941 tgggttgatc aactcttaaa tgctgtgaaa gtagaatata aagtaatcaa tccacataat
     9001 gtccagcctc agcctggcaa ggcaacgatc cttatgtgca atcattccag tgcttatgat
     9061 attcctttag gcttcaaagc attccctaat aactctattc gtatgcttgc caaacgagaa
     9121 ctctccaaaa ttccactttt agggaaggga atggctgcag cggaattccc ttttattgac
     9181 agaaaaaaca gatttcaagc cataaaggat ttggaatttg cccagcaact gatggaaagt
     9241 ggaattattc tatggattgc accagaagga actcgctcaa aagatggcaa actagcccca
     9301 tttaaaaaag gtggttttat cactgctatt caagccaagg ctactataat tcccatagga
     9361 attcgcggag cttttgatat tctccctgcc cgcaccttta atatacattt gaatcaaaaa
     9421 gcagaaattc atattggcga gcctattgat gcagctcaat atacaattga aaataaagag
     9481 gaattaatcg aaaaaaccca tcaatcaata aaaaagctgg ttggggagaa ttgaaaaaac
     9541 tcacattccg gtttgctgaa aaagatgact aaaaatacct acaataacta gaaaaaaaac
     9601 cacgctatac aaaaaataaa attgatttct acaatagaat aaacaatcca ggctatctgt
     9661 cttttaatat cggtactaac tgttttgaaa tttcctaaaa attcacctag actttaaaaa
     9721 agaaaatcaa tatggccgtg ccatgagcat taataaaata gaagaagaca gggatacctc
     9781 ttctgatctg gaaatgaaaa ccaagatgta cttagtacac cgaatggata aagccgggcc
     9841 attaacagtt aaagatgttc acatcctgat aaacaagtta agtaaagatt tgcagaaaaa
     9901 tcaattaaca ttaattgatt gtcaactaat aataaatctt gttgaatccc gctttacttt
     9961 ggtcaaacaa gcaacaagac atctacagtt ttttctaaat aaacttgatg gcaaatcaat
    10021 taacccaatc ttaatcccac tttcaaataa acccttttgg caaaatgaac cacatccatt
    10081 cgatcattat cgatctaccg atcaactacc tgaaaaagca gatatcgtaa tcataggtgc
    10141 aggtctaact ggcgcttcta ccgcatatca tctgatcgat gcagtcaaga aaagaaacct
    10201 tactgttgta ttgttagata aaggaaatcc agccactgaa gccagtggtc gcaatggggg
    10261 taattttgaa ttattacctg aaaactctgt gggaatctat agtggtttag tctcagagcg
    10321 gtttcgtttt ttaaaacgat gctaccccca tgttcatccg tcaatattac aaattgaaag
    10381 cgaataccat gcctcactgg tttttcaatt tgctttaaaa aatcgcaatc gctttaagga
    10441 aattgccaga aaagaacgta tttattgtga tctggcagca aaaggatggc tctacattgc
    10501 tcatactgaa gaggaagaac aagcaatttg tgatgaagtc actctggctg cccaacatgg
    10561 cgagcgcatc tacatttggt ctcgaaaaaa aattagagaa caattcggca tcaactccaa
    10621 gttcataggt cgatttattc cagatgacgg cacctacaat ccctttaaat atacgtgctg
    10681 tctgattaag gcggcaatca aaagtggtat caagctctat acgtttgtga aagttcaaca
    10741 aattaagagc atggatacag aataccatca attgaaaacc aatatgggtt tgcttaaggc
    10801 tcggcaagta attgttgcaa caaacgcatt tactagtgag ttgctcccgg aattgagcgc
    10861 gattaagcct caccaaagtc aaattgcaat taccgagtca acaccagatt actgtaaggg
    10921 tcgattaata accagcgaag atggaccact ctatctcaat caacccagag tcagatcaaa
    10981 aaatgggttg gtgccattac ttattggtgc cggtgacgac cgaccaatga aaaaccctta
    11041 ttatcgcaag cgttcaaaaa aagttcatga tcttattgtg cagcaaagag ataagtattt
    11101 cccggatctt aaagggaaac cattttcaac cgagtgggtt ggcgccttag cttttacacc
    11161 tgatcaatta cccgctattg gttacctgtc tccaggaata attattgtga tgtgctgtaa
    11221 tggatatgga ggaagctata ccacagcagc aggatttgcc gcagctgaaa ttgcattaac
    11281 tggaaaaaat cccccatggt tacccgcaga tgtattttct cctaaacgcc tcctgtctga
    11341 caagccgcta tttttggaag atagcgacag tttatggcgg attggaaaaa cactttgtac
    11401 aacacttaat aatctcaatc aacttctggc tgagtccctt tcatatcagt ccatgtatca
    11461 accttattct acaatttctt cattaccgca tgggcaccaa gagaagactt gcaatcttga
    11521 cccactaaga gtaatgcatc aattagcaat ctttagaact tttgcatatc aggaattaga
    11581 aatgttaatt cgctatacct cgcctgtttg cttaaataag ggagaaattc tttttaaaca
    11641 aggtgatgcg ggacattctt gttttattct gattcaagga aaaatcaatc tttattatga
    11701 gcatgctgaa ggctttactt caatggttgc agaactggag gccgggtcag tatttggtca
    11761 aatggcatta tttcttgaga ataaacgtat ggccacttgc caagctacag aaaactgttc
    11821 catgttagaa atcatggtag aaccttgtaa atcgcttttt attcagcaac aggctcttgg
    11881 aattaaatta ctccgcctct taaaccaggg agtcatcgat gcgttacaca aactcaataa
    11941 gcgattgaaa tacacttaac aaaaatcata aaacaaatat tgattttgat tgctttattc
    12001 agcactaaca atttacacac caagtgtagc tggccaattc atttcaaaaa tgtattctag
    12061 taattaaccc catacccata tgccatatta gatatcgcag cagctaaacc aatatgtctt
    12121 ggatcagaag cagccgttaa gacattactt tctttgtccc atgtaatggc ttgcatatct
    12181 ccataatatt gtttcaatgg cattaaatgg tatcccatag cttgtaaacc ctgttgaatt
    12241 gaacttggga aagtatcagg ctcaaattgc aaaacatctg gtaaatattg atgatgaaat
    12301 cgcatggctg aaaccatact gatagctcca tacccatcat aaaataatag tgaggcaagt
    12361 aaaaccatag taggaatacg gcttcctcca ggagtcccca ggatagctac acgccctggc
    12421 atctctagga aggttggagc catgctagat agcggccttt ttcttggttc aatggcattg
    12481 cgctcacttc ccaccaaacc aaagacattt tgctcaccca ctttggtaga aaaatcgtcc
    12541 atttcatcat ttaataatac tccggttccc ttcgcaacta cactggatcc aaaaatgtaa
    12601 ttaattgtca tagttgcagc aacgcgatta ccttccgtat caataatcga aatatgtgag
    12661 gtatttgagt gttttcctat cggaactgag ccttgcaaga ctttactatc aatcgcttta
    12721 tctggtgaaa tcaagcggct taattgtttc gcattatctg aggaaatcaa tttatccaga
    12781 gggatcttaa taaaatcagg atcacccaga aattgatctc tttgccaata agccaatctc
    12841 atcgattcaa cgacgtaatg aacccatttt aatttgggaa atgaagacaa aggaaatttg
    12901 gataatatat ttagcatggt taccaatgcc actccaccgg ctgaaggcag aggagctgtg
    12961 ataattaaca tattatgata agcactaatt aaaggtggcc tcactttaat tttatattga
    13021 gccagatcat caagagacca aattccacct gccgcattga caccttgaac tagcagagaa
    13081 gccgtttctc ctgaataaaa tccggcattt ccgtacttgg ctatcaatct gagtgttttt
    13141 gctaaatctt tttgaacaag tttttctcca atttcaaaag gcttatcatt ctttaaaaaa
    13201 atagcggcgg tatcgggata tttcttcaag ttttccaatc tatcatccag agtggagaaa
    13261 taacgaaatt gaggatctac aggaaaaccc tcttccgcaa gcttaatagc cggagctaat
    13321 gtcttgctaa gcggaagacg tccatagttc ttggctatat aaaccaaggc agctggctgc
    13381 cctggtatgg ccgcagataa accaccattt agagaaaggc cgggaatcac ttggccgtca
    13441 ggagccaaat acatatcctt acttgcggcc agaggagcta cctccctgcc atcaataaag
    13501 aggtttaaat gttccttttc ctgatgaagt agccaaaaac ctcctcctcc aagccccgaa
    13561 tgatatggct cgactactgc caaggcagca gctaccgcaa ctgcagcatc aaaagcattt
    13621 cctcctgctg ccaaaatctc caaaccggca tgagttgcca aggggtgagc acttgctaca
    13681 gcatatccag gagggtgttg tggaaatgtt tttgcattaa cctgataaat attacaaatt
    13741 aaaaaaaata aacaactcag ccatttatag ttatatttca ttttgtctct tttttaattc
    13801 tctaactaca ttgggcggaa caaattgaga aatatcacca tttaaggctg caatttcacg
    13861 aactaaagtg gatgagatat acattaaatg ctctgctgga gttaaaaata atgtttccac
    13921 ctttttagat agctttctat tcattccagc cagttgaaat tcatattcaa aatcagaaac
    13981 agctcgcaat ccgcgtaaaa taatgcctgc gctttgttct aatacaaaat caattaacaa
    14041 attatcaaaa cccacgactc tcacaccagt taaatgtcca acggactctt ccagtaaact
    14101 aattctggtt tcccaagata aataaggtcg tttgttctta ttactcgcta ctgcaactat
    14161 caattcagga aaaatagtgc tggcccgagt aataatatca atatggccat tggttacagg
    14221 gtcaaatgtt cccgggtaga ttgctttttg tttcatctca ttaattacca tttgcatata
    14281 gaagcagtct agcgtattgt aaattgaaaa actataaaaa aatggaattg aggtaagcaa
    14341 tgaatgcgat aaaacgtttg acaatcattt taatttgctt tctaacagca tgcgctcccc
    14401 caagaccctc tgaagagccg cctgcaaacc gtgaaattcc tcttgaagaa cgcaaagccg
    14461 aaacagccac tgtttcctca tgggaaatta aaggcgccct ggctgccaaa accaaagcga
    14521 aaggctggtc tgctactatg aattgggtac aacaaggggc caactcatac caaattcgtt
    14581 taatggggcc attaggtggt ggaaccgttc taatcagcaa aaaaggcaat actattacat
    14641 ttcaagatgg ttccaaaata actacttcga ccaatgccga tgagttatta ctaaaacaaa
    14701 ctggcattcg acttccagta aataacttgt attactgggt aagaggtttg cctgccccag
    14761 gtggagtcca atctgaaaaa catgaccaat tcaatcacct gatacaatta aagcaaaatg
    14821 gctataccat tgattttact aaatatactt cagtcaaggg aatagattta ccaagcatga
    14881 ttcgccttga aggaaatggt gtcatggtaa aggtagtgat aaagaattgg gttgtataat
    14941 ttaattcttg taatttattt gctttctgaa gttgacatcg ataaaaaagc actgcaaaat
    15001 acgcagcaca ttgcagggat gaatactatt tataaactca tggatgatta aagaatctac
    15061 aattataggg gtgtcgccaa gcggtaaggc acagggtttt gatccctgca tacctaggtt
    15121 cgaatcctag cacccctgcc attttcttgt tgattcaacc tagaatatga agttgaatct
    15181 aggtacaagt aaacagacgg cagaagataa cctagaagga tgcgtacttc gtatcgctca
    15241 aataataata atatcttttt ggctgtcttc tgctatgtgt ttaagttatg tgattaacct
    15301 aaatctaagg cttttgacac atgtccacga tgatgatttt tacagggaat gctaaccctg
    15361 aattagcact aaaaatttcc tcacatttgc aaatacctat tggcaaagcc accgttggta
    15421 ccttcagcga tggagagact atggttgaga ttttggaaaa tgtccgaggt aaagatgttt
    15481 ttgtcctcca atcaacttgt gcgcctgcaa ataataattt aatggaattg ctgattatgg
    15541 cggatgccct gagaagatca tctgctggtc gtattacagc tgtagttcct tattttggct
    15601 acgcacgcca agataggcga gtgcgctccg cccgtgttcc tatcacagct aaagtggtag
    15661 cagacatgat ggcttccgtt ggaatttgtc gagtccttac tgttgatttg cacgcggatc
    15721 aaatacaggg tttcttttat atgccggtcg ataacgtata ctcaactccg gtattgctgg
    15781 aagatataac aaaacaaaaa ttaaacaaca tcatgatcgt atcgccagat gtcggtgggg
    15841 ttgttcgtgc cagagcagtt gctaaaagac tgaatgacgc tgagctgtcg attattgata
    15901 aacgccgtag tgggccaaat aaatcagaag taatgcacat tattggtgag cccgccaata
    15961 aaaactgtat tattgtagac gacatagtag atactgctgg aacactttgt acggctgctc
    16021 aggaattaaa gaaaaacgga gcaaaaagcg taagagctta cattacacat cctgtgttgt
    16081 ctggtcccgc agttaacaat attaaacatt caggtcttga tgaggtggta gtaactgata
    16141 ccattccttt atcagccgaa gcacaaaact gtgaaaaaat cagagtggtc agtcttgctg
    16201 atatgttagc tcaagcaatc aaacgagtaa atgttgagga gtcagtaagc tctatgtttg
    16261 ctgaataaaa tgaaatattc attgataata aaaagggagc caaggctccc tttttattat
    16321 caatgtttct taatcccttg taccaacgta tttgtcatca aaataacgtc ttaatttagt
    16381 tcttaaagtt ccacgactaa ttcccagcat gattgcagcg cgggactgat tatacttgca
    16441 atgttccatt actgcacgaa atagaggggt ctcaatttct tcaagtacaa actggtataa
    16501 gtcaacagga tcagttccct taagctctga aaaatatttt tgtacggatt gagtcacgct
    16561 ttcggctagt gaagcagtgg tatctcctgc atcattactg attgttgtca ttattattag
    16621 ctcctccttt taaaagcggt atattaccga attgagcaat acgagacaag ggcagaacag
    16681 aaaaaactgc attaaaattc aatatatact gtttgcactt gaatcattgc tacgtttctc
    16741 accatagtgg attgaaaaga ggataagccg ctctcaacag cttatcctat atttaacgaa
    16801 ctactggaat ttcatcatag acggatcgaa tggtttccca ttaactgtga attttccttc
    16861 actcaaactc atctcaatga catagtcttt gccatcgaca gtaattaaac ctgattgctc
    16921 caactcatta atttgcttat ctacttgcat tgcagcgatt tgctcaggag taggttctgc
    16981 ttggtttgat tgaccttgac tgctttgcag ctgttgaatt aacgcttgtt gcatttctgg
    17041 ctgttttgcc atttgttgca taatcgattg ctgcatcagt tgcttcacta cctcgattgg
    17101 catttttaac ttggcattac cttgagtttt ttgaatcaat tcaaagggat tagaattatc
    17161 gcctttgggt aaagtaatta acaagttacc atcaatcatg ccttgtggaa gtttgagact
    17221 taatttggaa atttcaaact ccgcaccttt actaaatagc tttggcaatt ctggcaacat
    17281 tgccattaat gcttgttggc gctcttgatc tgtgcctctt tgcatcgcat tagcttgttc
    17341 attgatttta gccagtacct cagcatcaag gtttcttaag gacatctcga cttcaccagg
    17401 accgtaattt tgcccattgg cgaataacga ttttaaagca gcactaaaat gcgtgctgaa
    17461 gagattgtca tctatatcgc tgcttgagct taatgtcaat tcactaattt caaacatctt
    17521 ttgatcttta acaactacag caagtgatgg taatgaaaaa ctggcttgac ccaaatataa
    17581 ccctgtagtc gttttatgta agtcatactc gctgattacc ttacccaaag agagcttggt
    17641 atcatcttta gaaaactctg caccatcaaa ttcaatttcc ccttccactt tatccaaatt
    17701 ggaagacatt gatgtagtgc tgtccatacc catccaatta aatttaccat ttccatcttt
    17761 agcagttaat ttgaaagaag gaatggacaa ctccaccgtg cttttattca aataatttac
    17821 aaaaatgctc aaatccagct gaggcttttc tgaatcttga gaaaaaagct cattgaattg
    17881 ctgattatat ttttctggaa atggaaatac ggtttgtgca aagcccattc caaaacgtaa
    17941 ctgcttattc gcgtatataa tggggccatg ataaatctta atcggcattt ccatttgata
    18001 atcttgggca ggagtagttt gcaattttcc atcgtcgtcc tttactactc gctcaggtac
    18061 gtgtaaacgc catttaacta atgcatcaga gcaaaaccaa cctctcttat attgctgaat
    18121 gtcagcgaaa agcccattgg attgattaat tacctcgaca gtttttttaa ccgttctttc
    18181 ggttagaatt cccatgccat aatatccacc taggattaaa acagccagga taattactaa
    18241 tcctgtaagt tttttcatta ttatctccat gttattaatt tgcaaatagc attactcgag
    18301 cgcagcaaag tgctgcgaaa agtataacag caaataacct aaaagtgcga cagtctaatg
    18361 caagaagatt aaaacttaat gaaaaatatt ttcattaagt ttcataaatt tgatgtgttt
    18421 acagcaaaaa tcaaacaatg atggcatatt tatcatgcaa tgctcaatgt ttgatttttg
    18481 ctatcttaat gtcttatggg tttgcaataa gtctctttaa cagtaaacgc gaggattaag
    18541 gccactaagg aacataaggg aagtaatttt agaccggctt ggaaatccga taataataac
    18601 gtttcaacat tataggctct gggcccagac actatgtcta tgatacgccc tactaaaggc
    18661 tggaataagg ccccgacaaa aattgaagtc atattggtaa atgcgataga tgtaccaaca
    18721 acactcctgt catgtatttc tgtagaaaca gcgtatgaaa tcgcaactcc agtgtttgta
    18781 agaccaaaaa caaaaaatag tatgtaagcc gttgtttgac tcatttcagg aataaaaaca
    18841 aataaactgc tcaagatcac accacatact gcggaaataa tcatcaatgg cttacgacgt
    18901 cccattttat cagaaatcca acctgataat gggccactaa ttccccagcc tataaagatc
    18961 aatcctgttg caaatgcagc tgcgtgcgcc cctaaaccac ggccgaactg taaatatgcc
    19021 ggacctattg cctctccaat tacagcggtg gggccaaata aaaatccagc atacagcgca
    19081 ttaagccaag tttgtttatt tgataaaatg atctttaagc tatccaagat actgattcga
    19141 ttaacagaac gaatttcatt gcgatgctcg ccgggtttat cttgcacaaa ttgatacaat
    19201 aatcctgata aggcaataaa taagaacgcg ataatcagca tactatggcg ccagccaaca
    19261 ttactcacta aaaatgaaac aggggcctcc cctgcagcag caccaagcat tcctaacgct
    19321 tgagttaatc cagataataa tccgagcata gttggaggaa accatgaagt ggcaaggcga
    19381 agtgagcaaa tgaatgcaaa agcggcactg aaaccaatca acattcttcc aattgatgca
    19441 gtcagcagac catccgcaag gccaaataca cagcaaccaa aagcagtaac taatgacata
    19501 atagtcaata accatcgtat gcttaatcga tcaaccgtca atcctacagg aatttgcatt
    19561 aagatatatg gaacatagaa ggatgcagtt aatatgccga agccagcctc attaatgcca
    19621 aagtccattt gtagatatcg gtgcattaca ccgggcgcta ctctagctaa ataatcagaa
    19681 aaataaaaag cagctgccaa cccccaaact aaccacgcaa aacccaggta atttttacgt
    19741 ttataaattt ccactttaga tattcctatt ttggtataac caccacatgc tgtatttatt
    19801 taaaaaatga tcaataaatt aacaatgata acttatcatt atactgctgt tgtaacttat
    19861 ttcaacatat agtttatttt tacctttgcc ctattggtaa tgtattgagg atttaggata
    19921 taatcctaat ttttaatcac tcatcgtcat ccagtaaaac aaaaa
//
LOCUS       DAXLDT010000049        19774 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXLDT010000049 DAXLDT010000000
VERSION     DAXLDT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19774
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.049"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            342..842
                     /locus_tag="ACF2SP_001409"
     CDS             342..842
                     /locus_tag="ACF2SP_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HFD2359200.1"
                     /translation="MNTTSLSLTPLLRHSVGFERFNDLFESMRNADDSTYAYPAYDIE
                     KHGEDSYTITMAVPGFQESDLNIMVQNDKLTVSGRIQEKETKESEYLHRGIVTRAFEQ
                     TFRLADHMKVTGAEIKNGLLSISLIREIPEEAKPRIIPIKSISDQESNKKSKTIEPES
                     KKKISN"
     gene            1024..2544
                     /locus_tag="ACF2SP_001410"
     CDS             1024..2544
                     /locus_tag="ACF2SP_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFD2359201.1"
                     /translation="MKRGLFVHLKHDLPASIVVFFVALPLCLGIALASHVPLFSGIIA
                     GVIGGVVVGFISGSALGVSGPAAGLVAIVITSVETLGSWEAFLLAGVIAGILQLIAGF
                     LRGGIIAYYFPSSVIKGMLTGIGIIIILKQIPHLLGHDVTPLLLSMSDGELDINDIRE
                     ALDDINPEVVIISIVSLLTLIFWEKVLLKKHKLFEMIQGPLVVVILGIALNLFYEHRL
                     TGFSLDPSELVQLPKFNKASELFNYLTLPDFSLITDLKIYKIAILIALIASLETLLCV
                     EAVDKLDPYKRVTSTDRELKAQGVGNILSCLIGGLPITQVIVRSSANVAFGAKSKLSA
                     ILHGLLLFICVISIPEILNEIPLASLASILIIIGYKLAKPALFKQMYKMGWEQFVPFL
                     ITITGIIFSDLLFGITLGFSVAIFIILRHHFLNSHDIIKIRGKNKTQYCLKLAEEVTF
                     LNKGSIINELKNIPEGAEVIIDGSQSKIIDHDIKEVFQNFILNSKTKNIQVKLIGI"
     gene            2548..3174
                     /locus_tag="ACF2SP_001411"
     CDS             2548..3174
                     /locus_tag="ACF2SP_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFD2359202.1"
                     /translation="MWTLTKEQQQAITPEKAIELLKEGNKRFVSNLKLNRNLIQQVNE
                     TSQGQFPFAVILSCMDSRTPAELIFDQGLGDIFSIRVAGNILNDDILGSIEFACQVVG
                     VKLIAVVGHTQCGAIKGACDGVKLGNLTNLLNKINPVIQEAKKLDAKHDVHSPEFLNC
                     VTSLNVKHTMNEITQRSDIVHQLLNEKRIAIVGGLYQLETGEVQFFDE"
     gene            complement(3616..4587)
                     /locus_tag="ACF2SP_001412"
     CDS             complement(3616..4587)
                     /locus_tag="ACF2SP_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFD2359203.1"
                     /translation="MSLRILSLSDVKHSITMHQAISALESAFIQLANNTVKLPLRTGV
                     TIEEEKGLMLTMPGYLAQEKSLGLKVVSIFPENLTKNKPTITGFIMLLDATTGEAKVL
                     MDAAYLTSLRTGAVSGLATKYFARDNATQVAIIGSGAQAKTQLEAVASVRDIQQVFVW
                     SRNIKNAEKFANEFEDQFDMTICSSVAMAVKDADIICTATNSTEPLIHFGDISPHVHI
                     NAIGSHHPSMKEISHDVLSRAVVIADQLDAVLAESGEIISAVEHNHLKKESIIEIGQW
                     LLQKNTDCKDKLTVFKSVGLAIQDLSVANAVYQNALKKNLGELFSIS"
     gene            complement(4642..5088)
                     /locus_tag="ACF2SP_001413"
     CDS             complement(4642..5088)
                     /locus_tag="ACF2SP_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFD2359204.1"
                     /translation="MKSIVNKENEVAIIHKLIEEYANAARDKDIDKIMSHYAPDIRSF
                     DAVSQLQFKGADDYRKHWQTCLSFCPGPTVFEVHELETLVDNNLAVSYYLTYCGGTNE
                     KGETQGGWMRGTMVHCKMNGKWKIMHEHYSVPFDMKTGNTLFDLKP"
     gene            5462..6616
                     /locus_tag="ACF2SP_001414"
     CDS             5462..6616
                     /locus_tag="ACF2SP_001414"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2359205.1"
                     /translation="MPTKINENDITELNTSEFFKRKKATPRIVSKNDPQIIKSNQILK
                     LLNSKTRRKEAISAFLYSFADIDRNAVLDFIKVLQTYPADTPDTEIAQVIYQWYCSIG
                     KENKIGFKMTDQEYQLNKAQNIASIITANFSPRRKEQFKRKALLDIGAGDCAMTSLVS
                     ELLGMEANAIDIQSNIDWGGENSSDKKSKNDYMTKIHHHYTYDGRDLLGTLKGKKFAV
                     VMYNHSLHHFPSFQDQIESLKQVSQILEPGGILFLSEHANCFDDDIFDLSHILLNLRY
                     SIDKNQILTPSEAGDAIAKFKSEYQSHYFSKNILNAIATQLGFNLVKQEIRSATDVAK
                     ATFFCFVKKEPKIELPCSLRFFDTDAANGLPHHIEKSDSQQKKYSAESFK"
     gene            6789..8291
                     /locus_tag="ACF2SP_001415"
     CDS             6789..8291
                     /locus_tag="ACF2SP_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFD2359206.1"
                     /translation="MIKIIREMPHGVAALCLIQAFSTFSYAVQYSSLALFITKQLGIA
                     NLSANSIVGLFLSFSYIFQLIGGVIGSRFISNRLLFVTTILIQNMGLFVLAQANPSSL
                     NIGLSLFMVGSGLNVTSSNSMLTQCFIPQDDRRESAFFLSYAGMNVGYCIGYIACGFF
                     DYSNQYQYLFYASILTNSIALLIIIKNWAYLGDKNFPLPDLTKRNWYFIKNILAIAFI
                     TLLIPFMRVCFEFANFCNGLVIITCLFMFLIIFILGLKQKSSSDRKKMMAYLILAISS
                     VLFWMIYLTGPMGVTLFVKNNVDKHLFSFELATQWIKNINPVVIILGAPIMAMLINKL
                     KAKGFHVSVPVQFSCSFVFLALSFLCLIFGIICSNSYGYISVYWIIGHHILQGMSELL
                     LGPVGYALIGRIAPPKLQGILMGTWMLVTGVAASFSQYFSNAMVQSESVDPLITNQDF
                     LRAFKQLGLWSLLCAVLLYFVSGKITKIINGVQDNKPVNQSKAIPIPYSN"
     gene            complement(8296..8950)
                     /locus_tag="ACF2SP_001416"
                     /pseudo
     CDS             complement(8296..8950)
                     /locus_tag="ACF2SP_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9094..9630)
                     /locus_tag="ACF2SP_001417"
     CDS             complement(9094..9630)
                     /locus_tag="ACF2SP_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2359207.1"
                     /translation="MSAKQKDLERLLELKKKQEDLQVLNEKDMQERIKLERKYMEFLQ
                     MTSQQMQEELKKRGPVKEVDVKSKDIDPIIEDYKKLYSKESWYKEPETKDGKTHLTFP
                     SQEAAGNFFKDQAGKNRSFIVIDGATNKVLAYSNGDGKLYNGNGSVYQGGDFKASKED
                     FTSFKMPEREDPKMGMQL"
     gene            complement(9763..11193)
                     /locus_tag="ACF2SP_001418"
     CDS             complement(9763..11193)
                     /locus_tag="ACF2SP_001418"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFD2359208.1"
                     /translation="MDLSLLKKINDFEWQIPRHGKMLVPGVIFASEQLILDMDMKVYE
                     QLSNVATLPGIVSAAYAMPDAHWGYGFPIGGVAAFDPDDGGVISAGGVGFDISCGVRL
                     LSTGLKRDELESVKVSLADALFTHIPAGVGSRSGIHLTIKQLDDMMRGGAQWAVKQGY
                     GEVADLERIEDRGCVEGAIPDFVSEQAKKRQKNEMGTLGSGNHYLEVQEVRQIYCSDT
                     ATALNLHEGDIVVSIHCGSRGLGHQIGTDYLRSMVIESQHYGIKLADRELACAPIHSK
                     LGENYLGAMRAGINCALANREIITHFMRNVFQDILPRTQIDLIYDVSHNTCKEEFHEI
                     DGKKKRLFVHRKGATRALGPGHPQLPKAVSHIGQPVIIGGSMGTGSYVLAGVKGSEHK
                     SFSSACHGAGRAMSRHQAMKKWNGKDIVDKLAQQGILIRSGSYRGVAEEAPGAYKDVH
                     SIVDAAEMSGLAKKVAKLIPIICVKG"
     gene            complement(11227..11673)
                     /locus_tag="ACF2SP_001419"
     CDS             complement(11227..11673)
                     /locus_tag="ACF2SP_001419"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HFD2359209.1"
                     /translation="MISKRQNPLPLNKYWEHFTHEADIGVRGIGPTLTDAFEMGALAL
                     TNVVADATLIKPSLSIKIHCEAPNQEILFTDWLNAVVYNMQIHNMLFCEFDLKLNRFA
                     LDAVIKGEKVDQKKHQPVVEVKGATYTELKVYKNNDIWIAQCVVDV"
     gene            complement(11640..11924)
                     /locus_tag="ACF2SP_001420"
     CDS             complement(11640..11924)
                     /locus_tag="ACF2SP_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HFD2359210.1"
                     /translation="MGKFWSQRVTNTSNALDLEKGVFTWNDPQKIAASLLDSALNSNR
                     RKGTPYQSAMSMLNFYINRAGSKLAAGQKEILMEAKKELRLLAKDKTRYH"
     gene            complement(12008..13561)
                     /locus_tag="ACF2SP_001421"
     CDS             complement(12008..13561)
                     /locus_tag="ACF2SP_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase"
                     /protein_id="HFD2359211.1"
                     /translation="MRIFSRFSDRLVIFAFLVIIFVPGIGIFFEKQADEVRSLLNREP
                     HQLPPINIKKIGRTDFKGIENWFVDHTLFMTSLSKFWSHVVYQLGASIKPGQAILGKE
                     DWLFLGNDYAASIDQYTGRNKPAEEEILLKLSVLKQMNHLAKQNNIPFLVVIAPDKHE
                     IYPEYLPANVHKSSNKNRLDLLQEGMLARGIDFINLRQKEIEAKNTLGKQYGDLYLKG
                     DSHWNYVGAYVAYQAISDYMQQKGLQSRQLQFHFIPRETTYSDLTNFLQLTHIKSNNP
                     LPDVSNLKIDLFGRDINGKEIKLDDFQGNPNGVILIAPYENINKAVQNKQTCLLIGDS
                     FSESLSFYFHNDFYNTVRIHSGNTSWNLSDLIQKYHPDLIVYEKVERDLLYPLVNFQT
                     TANQMSLELPKQALAARGELDKFKIGPDTISVNGWAYIPDLDAGNGEVFLKLSMGTHT
                     YLYSMNKMQKQSVNLAFKQDGKHLDLSGFNGTISRKDLPSGLYKVSLIVLNDEVVGET
                     ELPGTYTLS"
     gene            complement(13564..14814)
                     /locus_tag="ACF2SP_001422"
     CDS             complement(13564..14814)
                     /locus_tag="ACF2SP_001422"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HFD2359212.1"
                     /translation="MINYLCGLLIEDSKKPGQRKIYLITSVSASLAMLMYFKYFNFFL
                     DSLNSVFDLNVMGISKIVLPLGISFYTFHALSYVVDVYWKKVPAERNFVTFVCYVSFF
                     PQLVAGPIVRYADIRHSFYRRYITRAGVRNGILRFCFGLAKKVLIANEVAKLADYVFY
                     LPANELTFWVAWLGAIAYALQIYFDFSGYSDMAIGLGLMFGFKYKENFNFPYNALSIQ
                     EFWRKWHISLSSWFRDYLYIPLGGSYKSRFRTQINLIIVFGLCGLWHGANYTFIVWGL
                     FHGFFLILERTHFGYFMKKLPKVIRHGYVLSVVTVGWVFFREDNIQSALVFLKTMFIP
                     AEFNLDSIAACINIILIFAFIVGIILALGFNSRIRDSLILSRNKNWIIAYKQSSKVLG
                     ILLLLVSILLLSTNSYNPFLYFRF"
     gene            15201..15839
                     /locus_tag="ACF2SP_001423"
     CDS             15201..15839
                     /locus_tag="ACF2SP_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359213.1"
                     /translation="MQDSFFSFLSSPINLLFLFVAFFLLFVTPWLWCMVNSTHNIHKL
                     LDLIGIKKKENSRVVNGLLLLPLLLITIFIEWLWPLENITLFPILASPYIKFNIIAVI
                     SVVILLILIQFDKKKSMGLWVLAYAAVLYLLYLIIIMATVVVGMNAFPASHHIFAPES
                     SITINRTIIYGLLIAHLIFIWLIISLKFTWRLLLMILYTLALSFAYFSFIVS"
     gene            16075..17175
                     /gene="wipC"
                     /locus_tag="ACF2SP_001424"
     CDS             16075..17175
                     /gene="wipC"
                     /locus_tag="ACF2SP_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipC"
                     /protein_id="HFD2359214.1"
                     /translation="MNRMALFIIFIIHCFFSQSFAEQEKPYNELYVKQANLKQYPREI
                     NSYPPGVEITIGDLHGNALKLLYFLIRNDVIKIDKEDYKLFVTIYQKNPNELTTKDLS
                     FFQIIVNSAEINTQHKIRFLGDDLCDRGMNDYYTLVIYKKLDQANVPFEVILSNHGNF
                     FLTAYERPEQSFNYNPYGEGENESTVQSMLNMGRLIDRGLIDKQDILEMIQYHYLKHI
                     VLPGYTHNKDKNELTIYTHAPIDLGIISALANDLQVPFKDSNLYELTKSLDAINSKIK
                     QWILSNTFTRHYKELNEAHNQTNTPSPIKQILWNRDYSILDRHANPNNKPYGINYVHG
                     HDSMPNVFDLDNLFGKGEDFYQGPYAVHITHS"
     gene            complement(17223..18446)
                     /locus_tag="ACF2SP_001425"
     CDS             complement(17223..18446)
                     /locus_tag="ACF2SP_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HFD2359215.1"
                     /translation="MIIYLLWLLAVTSFNFSTSTITVSKAEPQGNFLYSEIPSIKMPL
                     NEIKTLLQKEGNSLQPAVIDKVITTIQCANAYQVDRNNILTIIDYSMPSNQKRLWVFD
                     LDKKELLFHTYVSHGIKSGTLLTDKFSNKFDSKASSIGVYKTEQSYYGREGLSLRLVG
                     LDTKFNDNAFNRYIVMHGGWYMDEQFIKRYGRPGRSWGCPALPLPIKKQIIDTIKDNS
                     LLVIYYPSDEWFNKSKFLNCSKQKSDQVVINRLSETQAPVDDEIREDILFVDLNKNNS
                     REEHEPIITMSADAYERIFHSQPPLSRMLRRQINNAEYIALSKEEFNKLVLQGNREGL
                     GEIHFVIPVIIMEHGYYETQMQIVNMGKIKEVQPNSDTSRITQEPAKSYRIDFESKPA
                     LNLKTTNRFIRWLGL"
     gene            complement(18789..>19774)
                     /locus_tag="ACF2SP_001426"
     CDS             complement(18789..>19774)
                     /locus_tag="ACF2SP_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFD2359216.1"
                     /translation="PITDNKALMDLDVSVPKATGHDILVEIKAVAVNPVDTKVRRNVQ
                     PEPQQPKILGWDASGVVTEIGSEVTMFKPGDEVWYAGDLTRPGSNCEFHLVDERIAAK
                     KPKSLSFQEAAALPLTSLTAWELLFDRLQLSAEDKGILLITGAAGGVGSILVQLARQL
                     TQLTIIGTGARPESIQWILDNGAHHVIDHTKDMKAQLQNLHYSEVDYVISLTHTDQHA
                     KSLVEVLKPQGKFALIDDPQDLDIKLFKLKSISIHWEMMYTRSMFKTADMIKQHDILT
                     EVARLVDAGKIKTTIAECLGLINADNLRRAHALLESGTSRGKIVLNGFKSI"
BASE COUNT         6225 a         3905 c         3377 g         6267 t
ORIGIN      
        1 tacgcatctc ctcagcccaa acgggccttg gatatcgact gcttcttata taattcttat
       61 ccataactca cgttattcta ctgtttaata gtgtggtggt aactcattaa tttttcaaat
      121 actttagtat tctgatttcg atccatggaa gaattgctat tccgcatgtg tgttcctttt
      181 agacgataaa aatctattat tttgctactt gaaattgtat caatcgaccc tatttaagct
      241 tacaactaaa tagagtttta cacctcaatt gtgagacgtg gagcagctag taaagtgctc
      301 gggtaatatt aaacttggcc tcaaagttaa ggagatatat tatgaacact acctcattat
      361 cactaacccc gttactacgg cattccgttg gttttgaacg attcaatgat ttgtttgaat
      421 cgatgcgcaa tgccgatgat tcaacctatg cctatccggc ctatgacatt gagaaacatg
      481 gagaagacag ttacacaatc acaatggctg ttccaggatt tcaggagagt gatctgaaca
      541 tcatggtgca aaatgacaag ttaacagtta gcggccgcat tcaggaaaaa gaaaccaagg
      601 agtctgaata cttacaccgt ggcattgtga cacgagcctt tgaacagacc tttcgacttg
      661 cagatcacat gaaagtaaca ggtgccgaaa taaaaaatgg gctattgtct atcagcttga
      721 ttcgtgaaat accggaagaa gcgaaaccac gcatcattcc aattaagagt atttctgatc
      781 aagaaagcaa taaaaagagt aaaaccattg aaccggaaag caagaaaaaa atttccaatt
      841 aaaaaatgtg ccggatgata tggaaaatcc ggcacagatt tgagataaaa tagacagaat
      901 acttctgcac atgttctatt taataacttt ttacaataaa aataccagat aaactatact
      961 ttcaatttag aaagcacatt agtattacag aggctcaaac aaacaactga atatgaggcc
     1021 attatgaagc gtggtttatt tgtccatctc aaacatgact taccagcatc cattgttgtt
     1081 ttttttgtgg ctttaccttt atgtctgggc atagcattgg cttctcatgt tcctcttttt
     1141 tcaggaatta ttgctggtgt tattggtgga gtagtcgtag ggtttatcag cggctcagcg
     1201 cttggagtga gtggaccagc tgcgggactt gtagcaatag tcattacctc tgtagaaaca
     1261 ttaggcagct gggaagcttt tttgttagct ggggtgattg ctggaatatt gcaattaatt
     1321 gcaggttttc tgcgaggtgg aattattgct tactattttc cctcctcggt aatcaaaggc
     1381 atgctcacgg gaatcggtat cattattatt ttaaaacaaa ttcctcactt attagggcac
     1441 gacgtcacac cacttctatt gagtatgtcg gatggtgaat tggatattaa tgacatcaga
     1501 gaagcattgg atgatattaa tcctgaggtt gttattatca gcattgtttc tcttttaacc
     1561 ctgatttttt gggagaaagt gctgttaaaa aaacacaaac tgtttgaaat gatacaaggc
     1621 ccccttgtcg tggtcatcct gggaattgct ttgaacttgt tttatgaaca ccgactaaca
     1681 ggtttttccc ttgatccatc ggaactggtt caactgccga aatttaacaa agcatccgaa
     1741 ttatttaatt atttaacact gcctgatttt tcgctaatta ctgatttgaa aatatacaaa
     1801 atagcaatcc tcattgccct aatcgccagt ttggaaacac tgctctgcgt agaagctgtt
     1861 gataaactgg atccctacaa gagagtaacg tctacagacc gtgaattaaa agcccagggg
     1921 gtaggaaata tactctcttg cttgattggc ggactaccga ttacacaggt tattgtgcgt
     1981 agtagtgcca atgtcgcttt tggagcaaaa tccaagctgt ccgccatttt acatggctta
     2041 ttacttttta tttgtgtcat cagcatacca gaaatactca atgaaattcc acttgcatca
     2101 ttagccagta ttttgattat tataggctat aagttagcca agccggcttt attcaaacaa
     2161 atgtataaaa tgggatggga acagtttgtg ccatttctta ttacaattac tggaattatt
     2221 tttagtgact tgcttttcgg gattacttta ggcttttcag tagctatttt tattatcttg
     2281 cgccatcact ttcttaattc acatgatatt attaaaatcc gtggcaaaaa caaaacccaa
     2341 tactgtctga aactggcgga agaggtaact tttttaaata aaggaagtat aatcaacgaa
     2401 ttaaagaata taccagaagg tgcagaggta attattgatg ggtctcaatc caaaattatt
     2461 gatcatgata ttaaagaagt ttttcaaaat ttcattttga attcaaaaac taaaaacatt
     2521 caagttaaat taataggaat ttaaatcatg tggactttaa ccaaagaaca acaacaagca
     2581 attactccag agaaagctat tgagctctta aaagaaggca ataagcgttt cgttagtaac
     2641 cttaagttaa atcgcaactt gatccagcaa gtcaatgaaa catctcaagg gcaatttcca
     2701 tttgctgtta ttttaagttg tatggattca cgtactccag cagagctgat ttttgaccaa
     2761 ggccttggcg atattttcag tattcgcgtt gcaggtaata ttttaaatga tgatatcctg
     2821 ggcagcattg aatttgcatg tcaggtagta ggtgtcaagc tgattgcagt tgtcggccat
     2881 actcagtgtg gggcaattaa aggagcctgt gatggtgtaa aattaggcaa cctgacgaat
     2941 ctactgaata aaatcaaccc agtgattcag gaagccaaaa agctggatgc aaagcacgat
     3001 gttcatagcc cagagttttt gaattgcgtt acgagcctga atgtaaagca taccatgaat
     3061 gaaattaccc aacgcagtga tatcgtacat caattactta atgaaaaaag aattgcgatt
     3121 gtaggtggtt tataccagct tgagacagga gaggttcaat tttttgatga gtgatatcat
     3181 gatattaata acttccaata attagtcttt tcaactgtgc aacatttctg atacctaaag
     3241 cttttaccag gaattgatgc gggcttggtg cttatagtcc gcattcatga atcaatgata
     3301 ttttttattc aggtaataaa accaaaataa aattccgaaa tagagaaaaa ggggtttaat
     3361 aataaaatta acaactaata taaagcgtaa aaaaaattca ttggacaacc acaagacgcg
     3421 gcacaaagac aaaagtgaaa gcgttgaata atcagaagga atatacaata gtactgttac
     3481 tagcttaatc aaataaatac caactaacat aaccagtaaa gctatcagat gacgtaagca
     3541 atataaaacc gaaataagca ttttattttt tgcagaatga ttcatttaca ctcactctaa
     3601 tcggttttac ccttactaac ttatagaaaa taactcaccc agatttttct tcaatgcatt
     3661 ttgataaaca gcattggcaa cacttaaatc ctgtattgcc aagccaacgg atttaaacac
     3721 cgtgagctta tctttgcagt cagtattctt ttgtaataac cattggccga tttcaataat
     3781 ggattccttt tttaaatgat tgtgctctac tgcactaatg atttcgcctg attcagctaa
     3841 aacagcgtct aactgatcag caatcaccac tgctcggctt aacacatcat ggctaatttc
     3901 tttcattgag gggtgatgag aacctatggc attaatatgg acatgtggcg aaatgtctcc
     3961 aaaatggatc agtggttctg tgctattggt tgctgtacaa ataatatcgg catctttcac
     4021 tgccatggcg acagaagaac aaattgtcat atcaaattga tcttcgaatt cgttagcaaa
     4081 tttttcggca tttttaatat tgcgcgacca aacaaaaacc tgttgtatat ctcgcacaga
     4141 agcaactgcc tcaagttgtg ttttcgcttg agcacctgac ccaatgatcg cgacctgagt
     4201 cgcattatcg cgtgcaaaat attttgtcgc cagtcctgaa acagcgcccg ttcttaagga
     4261 agtgaggtaa gcagcatcca ttaatacttt agcttcacct gtcgttgcgt caagcaacat
     4321 aataaaacca gtgatagtag gtttgttttt agtcaaattt tcaggaaaaa tggatactac
     4381 ttttaagccc aagctttttt cttgggcaag ataacccggc atagtcaaca ttaatccttt
     4441 ttcttcttca atggtaacac ccgttctcaa gggcaacttc accgtgttat ttgccaattg
     4501 aataaatgcg ctttccaaag cactgattgc ctgatgcatg gtgatgctat gctttacatc
     4561 gcttaaagac aatatcctga gactcatatc aataaaccct atactttagt taactacgta
     4621 gtgactgaat agaatcacat cttatggttt taaatcaaat aaagtatttc ctgttttcat
     4681 atcaaaggga acagaataat gctcatgcat gattttccat tttccattca ttttgcagtg
     4741 taccattgta cctcgcatcc accctccttg cgtttcacct ttttcgttcg ttcccccaca
     4801 ataagtcaaa taataggaaa ccgccagatt attatcaacc aaagtttcca actcatgcac
     4861 ttcaaatact gtaggtcccg gacagaatga aagacaggtt tgccaatgtt ttctataatc
     4921 atcagctccc ttaaactgaa gctgtgaaac tgcatcaaaa gaacgaatat ctggtgcata
     4981 gtgggacatg attttatcga tgtctttatc acgtgctgca ttggcatact cttcaatgag
     5041 cttatgaatt attgccacct cattttcctt attcacaata gatttcatct ttatctcctt
     5101 taattgactt cttcctgaat tttaattgta gcaaattctg caaaaaacct aatgaattgt
     5161 taacctgcaa cagagtctgt tgcttttgca actcaagcca gcagaaaggt caagagatcc
     5221 tgttacagcc tgaattatct actatgggta attatataat aagcctcttt ccaaacatga
     5281 ctgcattaaa atctgaaaaa tgtgtcagga atattatgat ggtaaataca aacgattaga
     5341 ctcataacca ttttctccaa tacaaaaaaa tcataatgtc gatttatggt gcactgtgat
     5401 ttaattttaa aattaattag catataatga tcatttattt taatttaact ataagagtga
     5461 tatgccaacc aaaattaatg aaaatgacat tactgaattg aatacatctg aattttttaa
     5521 gagaaaaaaa gcaaccccca ggatcgtttc aaaaaatgat ccacaaataa taaaatccaa
     5581 ccaaatactc aaactattaa actccaagac tcgcaggaaa gaggcaataa gcgctttttt
     5641 atattcattt gctgacatag acaggaacgc cgttttagac ttcattaagg ttttacaaac
     5701 ctatcctgca gatactccag atactgaaat tgcacaagtg atctatcaat ggtattgctc
     5761 aattggtaaa gaaaataaaa tcgggtttaa aatgactgat caggaatatc agctgaataa
     5821 agctcaaaat attgcgagta ttatcaccgc caatttctca ccgcgcagaa aagaacagtt
     5881 caaaagaaaa gcgcttctgg atataggggc tggtgattgt gccatgacta gtttagtaag
     5941 tgaattatta gggatggagg caaacgccat tgacatccaa tcaaacattg attggggtgg
     6001 tgaaaatagc agcgataaaa aaagtaaaaa tgattacatg acaaaaattc atcatcatta
     6061 cacctatgat ggtcgagatt tattaggtac cttaaagggg aaaaaatttg cagtggttat
     6121 gtataatcat tcattacatc acttcccttc ttttcaagat caaatcgaga gtttgaaaca
     6181 ggtcagtcaa attttagaac cgggaggaat tcttttttta tcagaacatg cgaattgttt
     6241 tgatgatgat atttttgatc tatcccatat tttattaaac ttaaggtata gcattgataa
     6301 aaatcagatt cttacaccgt cggaagctgg tgatgcaatt gcaaaattta aatccgaata
     6361 ccaatcccat tacttttcga aaaatattct taatgcaatc gccacacaac ttgggtttaa
     6421 tctggttaaa caggaaattc gttctgcaac agatgttgct aaagcaacat ttttttgctt
     6481 tgttaaaaaa gaacctaaaa tagaattacc ctgttccctg aggttttttg ataccgatgc
     6541 agcgaatgga ttgcctcatc atatagagaa atctgactct caacagaaaa aatattctgc
     6601 tgaatcattt aaataaaata acctatacaa ttcaaacaga tttgattaac gaagcttaat
     6661 caatatttct ttcctaattt tactaaagta aagtttggaa agaaattgaa tggcaggttg
     6721 ttacattctt gaaggcaaat gattattgat ggtaaagtct attctcataa aatcagccaa
     6781 aatgtttgat gattaaaata attcgggaaa tgccccatgg agttgctgcg ctctgcttaa
     6841 ttcaggcatt ttcgactttt tcatacgctg ttcaatattc ttcgctcgca ctttttatta
     6901 ccaaacaact tggaattgcc aatttatcag ccaatagcat agtgggcctg tttttaagtt
     6961 ttagctatat ttttcaacta attggcggcg tcatcggaag ccgttttata agtaacagac
     7021 tactatttgt tacaactatt cttattcaaa atatgggttt attcgtttta gcgcaagcaa
     7081 atccgtcctc acttaacata ggcttaagtt tgtttatggt aggttcgggt ttgaatgtta
     7141 cttccagcaa cagcatgctt actcaatgtt tcataccaca ggatgatcgc agggaaagtg
     7201 cattttttct cagttatgcc ggcatgaatg ttggttattg cataggttat atagcctgtg
     7261 gtttttttga ttactccaac cagtatcaat atttgttcta tgcaagcatc ctgactaatt
     7321 ccattgcttt attgattatt atcaaaaact gggcctacct tggcgataaa aattttccat
     7381 tacccgatct cactaaacga aactggtatt tcataaaaaa tattctggca atagcattca
     7441 ttacactcct tattcctttt atgcgggtat gttttgagtt tgcaaacttt tgtaatggat
     7501 tggtaataat tacctgttta ttcatgtttc ttatcatctt tatcctgggg ctaaaacaaa
     7561 aatcctcctc cgacaggaaa aaaatgatgg catatttaat tttggcaatc agttcggttt
     7621 tattttggat gatttatcta actggcccga tgggtgtaac tctttttgtc aaaaataatg
     7681 tggataagca tcttttcagc tttgaattgg ctactcaatg gataaaaaat attaatcctg
     7741 tggtcattat tttaggagct ccaatcatgg ccatgctcat taacaaatta aaagcaaaag
     7801 gttttcatgt ctctgttcct gtgcaatttt cctgttcctt cgtgttttta gccctgtcct
     7861 ttttatgtct tatctttgga attatttgtt caaattccta tgggtatatc agcgtatact
     7921 ggatcatagg ccatcatatc ttgcaaggaa tgtctgagtt gcttcttggt cctgttggct
     7981 atgcgttaat tggaagaata gctcccccaa aactacaagg gattttaatg ggcacctgga
     8041 tgttggttac aggtgtcgca gcctcttttt cccaatattt ctccaatgcc atggtgcaat
     8101 cagaatctgt agacccccta attacaaatc aggattttct aagggcattt aagcaattgg
     8161 gactttggtc tttactctgc gctgtcttgt tatattttgt ttcaggtaaa ataaccaaaa
     8221 taatcaatgg ggtacaagac aataaacctg tcaatcagag caaggctatc ccaatacctt
     8281 actccaacta atcatttaaa aatccagagt attgctttgc tcatcgggtt cttccagact
     8341 tttagcaaca tccaggtaaa ttttcggatt gtactggtac aaacggaaga atatgctttg
     8401 ggaaaatttt tcctttgcct gcttgcgcct gtctacctct tgaatagaac atatctcatt
     8461 aagcagtgac caaaactcgg tgtaatccaa atgctttact tcagttgatg ataaactttt
     8521 tatatatcca tctacttcac ccgcatagtg gctaattctc tgaagcaata gcacatgact
     8581 attcataaga tctgtatcaa gtggtgttaa tacattgtac gatggagcta aaagcttata
     8641 caattcatta taaaatacag ccaaacccaa ggtattctta tcaatatcaa aggatacaat
     8701 agcatcacga atctgactta attcgtttaa aaaattgggg ggaatctctg gctgatgagt
     8761 gatttttctc aaccacagcg gagttttttt ccattgcgca atttgaatgc aaaccagatg
     8821 tttctctttt aactgctttt taatttcctc aaatttttct ctatcaatag ttagcatgtg
     8881 ttttctctta aaaaaggcaa gctataccct ggaagttagg gtatagtttc caaagttggg
     8941 aatatttgat accacttaag tggttttagg ccaaatgata catggcctga taatttgtgt
     9001 caaaaaaaaa tcaaagtggc ctttatttga aacaagagaa taaaccatca taacaagatt
     9061 atctgcatcg aatccgattt aaaccaaagc agattaaagt tgcatcccca ttttaggatc
     9121 ttctctttca ggcattttaa agctggtgaa atcttctttt gacgccttga agtcaccacc
     9181 ttggtaaaca ctaccatttc cattgtatag ttttccatcg ccattagagt atgctagcac
     9241 tttattggtt gccccatcga taacaataaa agatctgttt ttcccagcct gatctttaaa
     9301 gaaattgcct gctgcttcct gagacgggaa agtcaaatga gtttttccgt ctttcgtttc
     9361 gggttcttta taccaacttt ccttactata aagctttttg tagtcttcaa taataggatc
     9421 aatatcctta ctcttcacat caacttcttt tacaggacct cttttcttta attcttcttg
     9481 catttgctgg ctggtcatct gcaaaaattc catatatttc ctctcaagct ttattctttc
     9541 ttgcatatct ttctcattca aaacttgcaa gtcttcctgt tttttcttta attcaagtaa
     9601 tctctcaaga tctttttgtt ttgcactcat tctggatatc tcctcgtact tattgaacta
     9661 attttagtct aaaaatttat cgaaaattac tctgggttta ttttttttac agattcctta
     9721 caattatagg ttgctccatt cacctcaatc gataatggat tgttaacctt tgacacaaat
     9781 gatgggaatt aatttagcaa ctttttttgc caaacccgac atttcagcgg catcaacaat
     9841 agaatgaaca tctttataag ctcctggagc ttcctcagca accccacggt atgatccgct
     9901 acgaattaaa atcccttgtt gagccaattt atctactatg tctttcccgt tccatttctt
     9961 catcgcctga tgacgactca tagcgcgacc tgctccatga catgccgagc taaaagattt
    10021 atgctcagaa cctttgactc ctgctaatac ataggagcct gttcccatac tccctccaat
    10081 aatcacaggt tgacctatat gagaaacagc tttaggtagt tgtgggtgac ctggacccaa
    10141 ggcacgagtt gctccttttc gatgaacaaa taagcgcttt ttcttgccat ctatctcatg
    10201 aaattcttct ttacaagtat tgtgagaaac atcatagatt agatcaatct gagtgcgggg
    10261 taaaatatcc tgaaacacat tgcgcataaa atgagtgatg atttctctgt tggctaaagc
    10321 gcaatttatt cctgctctca tagcgcccag gtaattttcc cctagctttg aatgaatagg
    10381 agcacaagcc aactccctat ccgccagctt gataccataa tgttgtgatt caataaccat
    10441 ggaacggagg taatctgtac caatttgatg ccctaatcct ctggaaccac aatggatact
    10501 cactacaata tccccttcat gcaaatttaa agccgttgct gtatcggaac agtagatttg
    10561 cctgacttcc tgcacctcaa gataatgatt acctgaaccc aaggtaccca tttcattttt
    10621 ctggcgcttt ttagcttgct cagagacaaa atcagggata gctccttcca cacaaccgcg
    10681 atcctctata cgctctaaat cagcgacttc gccatagcct tgttttactg cccactgtgc
    10741 gcctcctcgc atcatatcat cgagctgttt gatagttaaa tgaatgccgc ttctactacc
    10801 tactccagct ggaatatgag taaataaagc gtctgcaagg ctaactttaa cagactccaa
    10861 ttcatctcgc ttcaatccag tagataagag cctcactccg catgaaatgt caaagcccac
    10921 accacctgct gaaataaccc ctccatcatc cggatcaaaa gcagcgacac cgccaatcgg
    10981 aaaaccatat ccccaatgcg catcaggcat ggcatatgca gcagaaacaa ttcccggcaa
    11041 agtagcaaca ttagataatt gttcgtaaac tttcatatcc atatcaagaa tcagctgttc
    11101 tgaagcgaaa ataactcctg gcaccaacat ctttccatgt cgcggtattt gccattcaaa
    11161 atcatttatt tttttcaaga gacttaaatc catcactgtt caccacatta attcctgcta
    11221 aggagtttat acatcaacga cacattgagc tatccaaatg tcattatttt tgtatacttt
    11281 taattcagta taggtagccc ctttgacctc gacaactggt tgatgctttt tttgatcaac
    11341 cttttcaccc ttaatgacag catccaaagc gaagcgattg agttttaaat caaactcaca
    11401 aaatagcata ttatgaattt gcatattata aaccacggca tttagccaat cggtaaataa
    11461 aatttcttga ttgggtgctt cacaatgtat ttttattgat aaagaaggct tgattagggt
    11521 tgcatctgca actacattcg ttaatgccaa agcccccatt tcaaaggcat ctgtgagtgt
    11581 aggtccgatg ccgcgaactc caatatctgc ctcgtgagtg aaatgttccc agtatttatt
    11641 taatggtaac gggttttgtc ttttgctaat aatcgcaact cctttttagc ttccattaaa
    11701 atttcttttt gcccggcagc taatttgctt ccagctcgat taatgtaaaa attcaacata
    11761 gacatcgctg attgataagg tgtacctttg cgcctattgc tgtttaatgc cgagtcaagc
    11821 aaagaagcag caattttttg ggggtcattc caggtaaata cccctttttc gagatcaagc
    11881 gcattgcttg tattcgtcac tctttgtgac caaaattttc ccattgattc gaccaactga
    11941 atctcataca cttatattat agcgatttaa tataagctct gctgttttga atcagggcca
    12001 ataccatcta acttaaagta taagtgcccg gaagctcagt ttcgcccacc acctcgtcgt
    12061 ttaagacaat gagtgatact ttatatagac cagaaggcaa atcctttcgc gagatagttc
    12121 cgttaaaccc tgacaaatca agatgcttac catcctgttt gaaagccaga ttaacacttt
    12181 gtttttgcat tttattcata ctgtataaat aagtatgagt tcccatcgac aactttaaaa
    12241 acacttcccc atttccagca tctaaatcgg gaatataagc ccagccattg actgatattg
    12301 tatctgggcc aatcttgaat ttatcgagct ctcccctggc agctaaagcc tgtttaggaa
    12361 gctcaaggct catttgattt gctgtagttt gaaaattaac gagaggatag agcaaatcac
    12421 gctcgacttt ttcatacaca atcaaatcag gatggtattt ttgaattaaa tcagacaaat
    12481 tccaggatgt attcccggag tgaatacgta ctgtattata aaaatcatta tgaaaataaa
    12541 aactgagaga ctccgaaaag ctgtctccta ttaataaaca ggtttgtttg ttttgtacag
    12601 ctttattaat attctcgtaa ggcgcaatga ggattacacc atttggattg ccctggaaat
    12661 catccaattt aatctctttt ccattaatat ctctgccaaa caaatcgatt tttaaatttg
    12721 aaacatcggg aagaggatta ttagatttaa tatgtgtcaa ctgcagaaaa ttggtaagat
    12781 cagagtaggt ggtttctctg ggtatgaaat gaaactgtaa ttgtctgctc tgtaaccctt
    12841 tttgctgcat gtaatctgat atcgcctggt aagcaacata agcaccaaca taattccagt
    12901 gactatcccc ctttaaataa agatctccat actgtttacc aagagtattt ttggcttcaa
    12961 tttccttttg tctcaaattg ataaaatcaa tacctcgagc aagcattccc tcttgtaaaa
    13021 ggtcaagtct gttcttattg gagctcttat gaacgttagc cggtaaatat tccggataaa
    13081 tctcatgttt atctggggca atgaccacca gaaaaggaat gttattttgc ttagccaggt
    13141 gattcatctg ttttaagaca gataatttca agagaatttc ttcctcggca ggcttatttc
    13201 tccctgtgta ctgatcgatg gatgccgcgt aatcattgcc caaaaacagc caatcttctt
    13261 tccctaaaat ggcttgccca ggtttgatgc tagctcccaa ctgatatacg acatgactcc
    13321 agaatttact caacgaggtc atgaacaaag tatgatcaac aaaccagttt tctatgccct
    13381 tgaaatcagt acggcctatt ttttttatat taatgggggg gagttgatga ggttctctgt
    13441 ttaatagaga tctcacttca tccgcttgtt tttcaaaaaa aataccaatc cctggtacaa
    13501 aaatgattac cagaaaagca aagatgacca gcctgtcaga aaacctggaa aaaatacgca
    13561 tcatcaaaac ctaaaataga ggaaagggtt ataagaatta gtgcttaata ataatattga
    13621 aaccagtaaa agcagtattc ccaaaacctt gctggattgc ttgtatgcga taatccagtt
    13681 tttatttcta cttaagatga gactatccct tattctgctg ttaaacccta acgcaagtat
    13741 aatgcccaca ataaaagcaa aaataagaat aatattaata catgctgcta tggaatcaag
    13801 attgaactca gcaggaataa acatcgtttt aaggaatacc agtgccgact gaatattatc
    13861 ttctctgaaa aatacccatc cgactgttac aacagaaaga acatagccat ggcgaataac
    13921 tttaggtaat tttttcataa aatagccaaa gtgggttctt tccagaataa ggaaaaaccc
    13981 atgaaataac ccccaaacaa taaaggtata attagcgcca tgccataaac cacacaaacc
    14041 aaatacaatg atcaagttaa tctgcgttcg aaatcttgac ttataactcc ctccgagcgg
    14101 tatatataaa taatctcgaa accagcttga taatgagata tgccacttcc gccagaactc
    14161 ctgtatcgat aaggcattat agggaaaatt aaaattttcc ttgtatttaa acccaaacat
    14221 caaaccaagg cctattgcca tatcagaata gccggaaaaa tcaaagtaga tttgcagagc
    14281 ataggctata gccccgagcc aggccaccca aaaagtcaac tcgttggcag gcaaataaaa
    14341 tacataatca gcaagctttg ccacttcatt agcaataagg actttttttg ctaaaccgaa
    14401 acaaaaacgc agaattccat tgcgtactcc tgcacgagtt atatatctcc gatagaatga
    14461 atgtcttata tctgcatagc gaacgattgg tcctgcgacc aactgcggga aaaaagatac
    14521 ataacaaacg aaagtaacaa aattacgttc tgccggtact tttttccaat acacatcaac
    14581 tacataactt aaagcatgga aagtataaaa acttattcca agaggcagca caattttact
    14641 gattcccatg acatttaagt caaagacact gttcagtgaa tcaagaaaaa aattaaaata
    14701 cttaaaatac atgagcatgg ccagtgatgc gctcacagag gtgatcaaat agatttttcg
    14761 ttgtcctggt tttttgctgt cctcaatcaa taggccacat aggtaattta tcaaaatgca
    14821 cgccagcata ataaagatat gatctttctc accccaataa taaaaggaca agctaaagag
    14881 cattaaaaca aaattttgcc acttgatact tttaaaacag actagataag tcaggttaag
    14941 gcacggaaga aataaaaaaa gaaatattaa agatgaaaaa accattctat ctccgtgaag
    15001 gcatttgcgg aattaattaa acttgtatta actgttcaag acgttagatt catcgcgtga
    15061 agcgcattat agggaagtgt tatccgttgt gcaaatagaa tacgataaaa agaaccatct
    15121 gcaaaatctg aattgaagct gccattataa cattcattag ctatacttaa cctaaggcag
    15181 cattcgttga tgaattaata atgcaagatt cctttttctc atttttatca agccctatca
    15241 atttactctt tctttttgtt gcattttttt tgctttttgt aacaccatgg ctctggtgca
    15301 tggtgaattc aactcacaac attcacaagc tacttgacct tatcgggatt aagaaaaaag
    15361 aaaactcacg agtagtgaat ggactgttac ttcttccact gttactgata acgattttca
    15421 ttgagtggct gtggcctttg gaaaatatca cgttgtttcc tatactggcc tcaccctata
    15481 ttaaatttaa tattattgca gtcatttcag tagttattct tttgatttta atacaattcg
    15541 ataaaaaaaa atcaatgggg ctttgggtgc ttgcctatgc agctgtactt tatttattgt
    15601 atttgattat catcatggcg actgtagtgg tagggatgaa tgcttttcct gcaagccatc
    15661 atatttttgc ccctgaaagc agcataacaa tcaataggac aataatttat ggtttattaa
    15721 tagctcattt aatttttatc tggctaatca tatcgcttaa attcacatgg cgtttattgt
    15781 taatgatact ctatactctg gccctgtctt ttgcttattt ctcttttata gtctcttgaa
    15841 taagctgttt cttttatttt attgctgcgt cgcaaacgtt tctgtggtgc tcatttgaca
    15901 tagtaaactt cactccccaa acgtttgcac cttgccctaa agcaaaataa tcaggttggg
    15961 caagaggtct aatataaagc cattgccttc atgataaaac caaggtatac ctagcttacc
    16021 agattacagt catcgacaca gaacaataaa aaatttttac aggatgtagc attcatgaat
    16081 cgcatggcac ttttcatcat ttttattatt cattgttttt tttctcaatc atttgccgag
    16141 caggaaaaac catacaacga gttgtacgtc aaacaagcaa acttgaaaca gtatcccagg
    16201 gaaatcaata gttatccacc tggtgtagaa atcactattg gagatttgca tggaaatgca
    16261 ttgaaattgc tctatttttt aattcgcaat gatgtgatta aaatcgacaa agaggactat
    16321 aagttatttg taactatcta tcagaaaaat cctaacgaat tgacaacaaa agatttgtct
    16381 tttttccaaa ttatagtcaa ttccgctgaa atcaataccc aacacaagat acgttttcta
    16441 ggagatgatc tatgtgatcg tggtatgaat gactattaca ctttggttat ttataaaaag
    16501 ctcgaccagg ccaatgtccc ttttgaggtg attctatcca atcatggcaa ctttttttta
    16561 acagcctatg aaagacctga gcaatctttt aactataatc cttatggtga aggtgaaaac
    16621 gaatccacag tgcaatccat gctaaatatg ggaagactga ttgatcgggg attgattgat
    16681 aaacaggaca ttttggaaat gattcaatat cattacttaa agcatatagt acttcccggt
    16741 tatacgcata ataaagataa aaatgagctt accatttata ctcatgcgcc aatcgattta
    16801 ggaataatct cagcattagc caatgatctg caagttcctt ttaaagactc taacttgtat
    16861 gaattaacca aaagtcttga cgcaattaac tccaaaataa aacaatggat actctctaac
    16921 acgttcacta gacattacaa ggagcttaac gaagcacaca atcagaccaa cacgccaagc
    16981 ccaattaagc aaatattgtg gaatcgtgat tattctatcc tcgatcgcca cgctaatccc
    17041 aataacaaac cctatggcat taattatgtt catggccatg actctatgcc caatgtgttt
    17101 gatctggata acttatttgg aaaaggtgag gatttttacc aagggcctta cgctgtgcat
    17161 attacacaca gctagctgtg aataaactca accctgtcat taaacagggt aacaaattga
    17221 aattatagcc ctagccaccg aataaagcgg tttgttgttt ttaaattgag ggctggtttt
    17281 gattcaaaat ctattctata gcttttagca ggttcctgtg taatacgaga cgtatcagaa
    17341 ttgggttgaa cttccttaat tttacccata ttgacgattt gcatttgagt ttcataatac
    17401 ccatgttcca taataatgac aggtattaca aaatgaattt ctcccaaacc ctccctattc
    17461 ccctgaagaa ccagcttgtt aaattcttcc ttactcaaag caatatattc tgcattgtta
    17521 atttggcgtc ttaacatgcg ggacaaagga ggttgagaat gaaaaatacg ctcataagca
    17581 tctgctgaca tagtaatgat aggttcatgc tcttcacggc tgttattttt attgaggtca
    17641 acaaacaaaa tatcttcccg tatttcatcg tcaacaggag cttgggtctc agaaagcctg
    17701 ttaataacga cttgatctga tttctgttta ctgcaattta aaaatttcga cttattaaac
    17761 cactcatcgc taggataata aataaccagt aaagaattat cttttatagt atcaataatt
    17821 tgctttttaa ttggcaaagg taacgcagga cagccccaac tacggccagg ccgaccatat
    17881 cttttgatga attgttcatc catataccat ccgccatgca taacaatata gcgattaaag
    17941 gcattatcat taaacttggt atccaaaccc actaatcgta aagacaaccc ttctcgacca
    18001 taataggatt gctctgtttt ataaacacca atactgctgg ccttactatc aaatttattt
    18061 gaaaatttat ccgttaaaag agtccctgat tttatcccgt gagatacata agtatgaaat
    18121 aataattctt tcttatccaa atcaaacacc cataaccgtt tttgattaga aggcatggaa
    18181 taatcaataa tagtcaatat attatttcta tccacttgat aagcattagc gcattgtatg
    18241 gtcgttatca ctttatcaat aacggcaggt tgaagagaat taccctcttt ttgtaacaat
    18301 gtttttattt cattcaaagg catcttgatt gacggtattt cactgtataa aaaattccct
    18361 tgaggctccg cttttgacac tgtaatcgta gatgttgaaa aattaaatga tgttactgct
    18421 aataaccata gcagataaat aatcatagtt tttccttgtg attgagttga tcttatattt
    18481 tttattccct gtattaatta atatagaaca cgacaaaaca ccatgtccaa tttttaatta
    18541 aaagatattt ttttaacaca aatagatttg cgtaaaacac caaggccaag gcaagaaatg
    18601 tttttaatga gggatttaga taaactaaac gagtgatcct ccgggtactg ctgcttgtca
    18661 gcaataccaa ccaagcactc tctggatgcc tcagacaagc tgaggcacgt aggctattta
    18721 gagaatcatt taaaaatcaa gtgactctct ctgtttcatt ccaggcgtcg tcgctgtttc
    18781 gagaataact aaattgactt gaatccattc aaaacgatct tgcctctgga agtaccactt
    18841 tccagtaaag catgtgctct tctcaaattg tcagcattga tcaatcccag acactcggca
    18901 atagttgtct tgattttacc ggcatccacc aatctggcaa cctccgttaa aatatcatgt
    18961 tgcttaatca tatcagctgt tttaaacata gagcgggtgt acatcatttc ccagtgaata
    19021 gaaatactct ttagtttaaa taacttaata tccaaatcct gagggtcatc tatcaatgca
    19081 aatttccctt gaggtttcag gacctcaact aacgatttgg catgttgatc ggtatgggtc
    19141 aagctgatca cataatccac ttcagaataa tgtaaattct gcaattgggc cttcatgtct
    19201 ttagtgtgat caatgacatg gtgggcccca ttatccagga tccactgaat tgattcaggg
    19261 cgggctcctg ttccgatgat agttaactgg gtaagttgcc ttgccagttg taccaaaata
    19321 gaaccaaccc ccccagccgc tccagtaatt aatagaatac ctttatcttc tgctgataat
    19381 tgcaatctat caaataaaag ctcccaagct gttaaactgg ttaaaggtaa tgcagcagcc
    19441 tcttgaaaac ttaacgactt tggttttttg gctgcgatgc gttcgtcaac aagatgaaat
    19501 tcacaattag accctggtct ggttaaatca ccggcatacc acacttcatc acctggttta
    19561 aacatagtca cttccgatcc aatctcagta actaccccgg aagcatccca gcctaatatt
    19621 ttgggctgct gaggttctgg ttgaacatta cgacgcacct tggtatctac gggattcact
    19681 gcaacagctt tgatttcaac taaaatatca tggccggttg ctttgggaac agaaacatcc
    19741 aggtccatca atgctttatt atctgtaatg ggta
//
LOCUS       DAXLDT010000050        19513 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXLDT010000050 DAXLDT010000000
VERSION     DAXLDT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19513
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.050"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1103
                     /locus_tag="ACF2SP_001427"
     CDS             <1..1103
                     /locus_tag="ACF2SP_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359217.1"
                     /translation="ELFVLINMAQSYDVDEQLKAIMSWLYQFNPILKASNKGLLSFYT
                     ELEPKFHSEGHLEKRVETGTDDLLYRIKTFLVSLFVTPFEVFPFSGKTISFWDIENVI
                     FSEGQEIYNQFAPFIMTNKLDTLIAVYKKIMDEHIIPCQKNKHICKWLTHYQSTLDWY
                     QRVEAGELSKMDVYWFDPELLFHVLVHFRLINKSLGEKIVNFLDELIHTYAQNNNEFQ
                     IQLRVNILFSRFLKSLDEQQRRKLILTLSLFDPVEAKSKFLTNCIHYVTNRLCQISMH
                     QLDSSPNFFGTYQCIDSKKLLINKTDVKQVSAILEAFKEMLHSLEERCNPEQLENMLI
                     FLRSISRPILTVAEIEEAQQSARVIDYIGAPT"
     gene            1264..2433
                     /locus_tag="ACF2SP_001428"
     CDS             1264..2433
                     /locus_tag="ACF2SP_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2744 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359218.1"
                     /translation="MSNLIELALRNAALDRTKLLSQVQKIEFLNGYDPRFVEGLISLE
                     MIGAIDEYVASLMYVKMTDKYTQEEKGQLTDTILTINNDRLISCQYRFPQLYQKLMQD
                     QFLSTFFSSSHLLPNIAPGTNNQSYVTVSTNNKMKMESAKLYGHYEYYQCVVALITQI
                     DGSRYFYHLNPNRIFGDYMGSAPLKLSGSELKDVKDITFILHPRSMVDYRELQRLQER
                     NIVFKTVMLPVGAKSVSLSYDTDKDVLTVNNLEAGQTVTYNNLLQTPQYNRYQYTPEQ
                     YFMTQLYRGQTWAEALGEALNKYIVTRQEGATWWDLKPLFFNRETTKNNMIAAARIKK
                     EVQAGATSFDFTGVSRNGLLNQLLTTAVDYQLEYQRGLQNGMLSAEDLTTSAALR"
     gene            complement(2468..3445)
                     /gene="rsgA"
                     /locus_tag="ACF2SP_001429"
     CDS             complement(2468..3445)
                     /gene="rsgA"
                     /locus_tag="ACF2SP_001429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFD2359219.1"
                     /translation="MSKRRINKQQSARIAKIQKNYHESMENNSDNLADGLVITRFSRH
                     AEIEDNQGKRILCSIRPNLDTLVAGDRVIWQIEGEKQGVVVSIYPRGSLLARPSQKGL
                     KKPVAANITQLVVVIAPKPEISWPLLDSYLIMAEILQLHVAIVLNKIDLPCQTLQQRL
                     MTDYQLLNYPVIMASNQTPDTIVPLKQALNNQISVFVGQSGVGKSSLISSLLPHEENI
                     AIDKISMVSELGKHTTRNSRYYHLPSGGALIDSPGVREFSLWDIDCSMIAQGYKEFKP
                     YLSQCKFRNCTHIDTPQCAIIDALNKGKISTQRYENFTKLCAQFNSLKE"
     gene            3508..4740
                     /locus_tag="ACF2SP_001430"
     CDS             3508..4740
                     /locus_tag="ACF2SP_001430"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFD2359220.1"
                     /translation="MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGR
                     DFPVFLHPETKEEYALARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDE
                     QGNLIDPYQGQRDLEEKLLRHVSPAFAEDPVRVLRVARFASRFHHLGFKIANETRLLM
                     YSMVKQGELAHLIPERVWQEWQKSLQEKNPEQFILSLRSCDALRVVLPEINSLFGVPN
                     PHQYHQEIDTGIHSLMALRASSELSEEPLVRFAALVHDLGKASTPIQDWPKHHGHEEE
                     GTKLIRALCARLRIPNDYRDLAVTVARAHLNIHRVCELRPNTIVKLLEQVDAFRRPQL
                     FHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVTPQTFIVQGYEGKAIKEAMHQ
                     SRVACVERIMTSWKSNEK"
     gene            4730..5293
                     /gene="orn"
                     /locus_tag="ACF2SP_001431"
     CDS             4730..5293
                     /gene="orn"
                     /locus_tag="ACF2SP_001431"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFD2359221.1"
                     /translation="MKNNQNLIWIDLEMTGLEPEQDRIIEMATIVTDPQLNILAEGPV
                     IAVSQPKILLDSMDAWNTKQHNQSGLVKRVLESNVSESQAEQLTIEFLKQYVDKGKSP
                     MCGNSICQDRRFLYKYMPELAAYFHYRNLDVSSLKELVLRWRPELMNGVVKESKHLAL
                     DDIKDSINELIYYRQHFINLPEVKNDK"
     gene            5283..5945
                     /locus_tag="ACF2SP_001432"
     CDS             5283..5945
                     /locus_tag="ACF2SP_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFD2359222.1"
                     /translation="MTNRERLELPNGAKKLLLHSCCAPCSGEVMEALIFSEIDFTIFF
                     YNPNIHPLQEYEIRKEENIKFAQKHNIPFIDADYDKENWFARAKGLEWEPERGKRCTM
                     CFDMRFERTALYAYEHDFPVISSSLGISRWKDMNQINDCGVRAASHYPDLVYWTYNWR
                     KNGGSERMYEIAKREAFYKQEYCGCVYSLRDTNAWRKQNTRDRIKIGLNYYSASKNEG
                     EL"
     gene            5942..6865
                     /locus_tag="ACF2SP_001433"
     CDS             5942..6865
                     /locus_tag="ACF2SP_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFD2359223.1"
                     /translation="MSKHSHDGAYHHEHGEVTYDRAFIIAITANGLFVVMQIIFAYLA
                     NSTSLLADAFHNLGDVLGLILAWVATGLMKRKPTHKATYGFKKTSILAALANGALLVF
                     TCGIIATDAMYKLFSPTEVQAVSVMIVAAIGIVVNFSTALLFLRGSDDLNIRGAYLHL
                     FYDALISVGVVLSAGVLYLTGWLWIDPVVGLLIALVILKGTWSLFADSFRLIIDGVPR
                     DISWIKVSDFLLAIPGVKSIHDLHIWAISTKENALSVHLFMPEESLSDDLRRELVEKL
                     KHDFSIQHVTIQVEKTEAECNDACHNPRIII"
     gene            complement(7001..8188)
                     /locus_tag="ACF2SP_001434"
     CDS             complement(7001..8188)
                     /locus_tag="ACF2SP_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HFD2359224.1"
                     /translation="MIRVLFAFIILLLSVFLGIQLNKDPGYVLIAINNWTIETTLWVS
                     AFGLFFLFFLIYGLVQLCNKITRMPGMVNKWHSRRLAQKAQATTRKGLIEYSEGYWQK
                     AKNHLIQALPNTDTPLLNYLTAARAAQKMGDSKLRDHYLREAQQSMPEAKIAVELTQA
                     QLQLANHQWEQALATLKHLQDLAPRHPYVLKLLMNLYEEIKDWQQLIALLPDLKKNHI
                     ISDQAFEQLQKNTYLQAMIDLAKHNQSDGITKLYQTLPKTMVNDTAITAEYARFLIRN
                     QDYDQAEYHLRRCLRKELDNRLIELYSLLPCEENQLLFAESLLKKNPHSAALYLCLGR
                     MCLSRHLWGKAKHYLEQSIELNPTPVAFIELGKLHEKLNDPLSSGSCYKKGLELITKD
                     ELS"
     gene            complement(8191..9315)
                     /locus_tag="ACF2SP_001435"
     CDS             complement(8191..9315)
                     /locus_tag="ACF2SP_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFD2359225.1"
                     /translation="MADSNEEQKKTIQPQQSSKVEPDINQQMKAPFFVSKYLSLIISL
                     TSLLAFLAIIISIYSIQLDKQFQNHQLVENKKLTDELGRIKAEQNTVQKLLDNNANHL
                     QQIQSDLTSKMDSLNKELQTAMKQKLYQNNDWLLLKARYYLELAQINVHWSDNFNTSV
                     ALLQQADALLKGMNIPKIFTIRQTIAKEIAQLKSISVVDITGILSQLDAAQAAINNLS
                     IQSVVDQQEVLNHITKSEESGKTGWRNRLQDSVNFLEKLVVIRRHNENIQPLISPLYE
                     SAIKENIRLNLQEAQWAILNNNPAVYQFALNQAIANLKRVFNESSQNTDALIKQLSTL
                     QNVKLTQEKPVVGQAIPLINQMIDNKELLVDQANSKKGEN"
     gene            complement(9319..10071)
                     /locus_tag="ACF2SP_001436"
     CDS             complement(9319..10071)
                     /locus_tag="ACF2SP_001436"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFD2359226.1"
                     /translation="MTKSLNGLRILNTRPREQAQVLNKKIIESGGIPIDCPTMEIMAS
                     ENNWINNLPDLTSVNQAIFVSANAVRYFMMELASKNINWLNSIRVAAIGKGTSRVLNE
                     FNIQVSDVPELPDSEHLLTLNSLQQIRNQTILLVKGEGGRQLIEEYLMLKGAKLCLLS
                     VYKRVMPTIDQEFLNSLWRDDLADIILLTSEQSIHNLFKMFSIEGQMWLQNKPCLVIS
                     DRLAKVASLFGIKKILISHPERMIGTLFDYKD"
     gene            complement(10068..10997)
                     /gene="hemC"
                     /locus_tag="ACF2SP_001437"
     CDS             complement(10068..10997)
                     /gene="hemC"
                     /locus_tag="ACF2SP_001437"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFD2359227.1"
                     /translation="MSAKIIRIATRQSPLALWQANHVREMLVKQWPNLSIELLPMITS
                     GDRFLKDKLLSAGGKGLFVKELEEALLDKRADLAVHSTKDMPAQLPDGLSLAAICKRD
                     NPFDALISPRFKSLDALPKNAIIGTSSLRRQSQLLAYNPNLQIKTLRGNIHTRLSKLE
                     SGEYQAIILAAAGLERMGLAHHITQLIPDDIMLPTCAQGALCIECRTDDLEIQELIHG
                     LNDPISALCVHTERRVNAKLGGNCHIPFAVYCTITAEKLLLLRAKVLNLDGSQMIDDE
                     QQGKIEEAEIIADRCTESLMTKGAMSLLSTIPS"
     gene            complement(11039..11200)
                     /locus_tag="ACF2SP_001438"
     CDS             complement(11039..11200)
                     /locus_tag="ACF2SP_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359228.1"
                     /translation="MTINDRIKKFFSKEIGGWHYKPILKPVGMLIYKSIARQDLRTYY
                     LNGVLFKLE"
     gene            11388..12677
                     /locus_tag="ACF2SP_001439"
     CDS             11388..12677
                     /locus_tag="ACF2SP_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD2359229.1"
                     /translation="MSQLKKIVKHLDESMQRSLSNSAHQLVAVGAIAFVGFPLFYVIW
                     AFWLPQPYENLPLRLIGSLLGLGLMLTPYWPLKWKQYLSWYWFLTLLFTLPYFFTFLF
                     LMNQASVISAMSLLCGVFLLVLLVDLLSLSIVLILGFSLALVSYYLVSPQMYFGEEHI
                     QMTLVIIFTIIAGSTLNYKTAMLQQQKLAGMAAAAGMIAHELRSPLLGIKSGAQALVQ
                     YLPRLIDGYQLAKKQELISSPLRDRLLLQLGGVSNRIVSEIDYANTIIDMLLVKASRD
                     NILQNCILETCSIAECLSEAITRYPFQSPRERTLITWTGDFQFKGSKLLMQHILFNLI
                     KNALYVIAMAQRGEINIWTESGDKYHYLYFKDTAKGMSKQQLNRLFNHFYTTTFMGTG
                     IGLSFCKLVMNAFGGDIVCESKEGEYALFILSFPGSV"
     gene            complement(12765..13328)
                     /locus_tag="ACF2SP_001440"
     CDS             complement(12765..13328)
                     /locus_tag="ACF2SP_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFD2359230.1"
                     /translation="MANSQEFYQPPVDELKRNWGWILGFGIFFLILGCVGLGMVVGLT
                     LVSMFFFGALLIIGGISHIIDVFKYKEWKGMIWQALIAVLYIAAGCIVLYDPFLASTL
                     ITAFLAAVLIVIGLTRMIMAFALKDSKGWGWLLLAGITAIILGILILMQWPVSGLWII
                     GLFIAIELIVTGWTYIFIAISVKMAKL"
     gene            13609..14634
                     /locus_tag="ACF2SP_001441"
     CDS             13609..14634
                     /locus_tag="ACF2SP_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2359231.1"
                     /translation="MQHFSIPTCYFPSTAVFVDDSRDFLLNFVLQLDEGLAYRVFDSP
                     FEALDCIKQKRCELELLSERCLSEYTEAKNCPLTNHTINLDLAAIHAEVYNSRRFSEI
                     SVVVVDYAMPGMDGLEFCRRIEDTNIKKILLTGQADEKLAIAAFNEGLIHRYIKKSDP
                     DVASLITQSIHDLQLQYFQSMSDMIVRMLSVTSPNCLHDKKYAELFWRLCREKGIVEF
                     YLADNSGSFLMLDDDANISFLIVKNEADMQLHYDLALDNGASGDVLDQLHNGEKIPCF
                     WQSNLVTPQWNEWSNCLVPANRFVSDETYFYAYVQGAVLFDVRLDKILSYHQYLEELD
                     AEEMFLN"
     gene            complement(14715..15965)
                     /gene="lqsA"
                     /locus_tag="ACF2SP_001442"
     CDS             complement(14715..15965)
                     /gene="lqsA"
                     /locus_tag="ACF2SP_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing autoinducer LAI-1 synthase LqsA"
                     /protein_id="HFD2359232.1"
                     /translation="MSNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVN
                     KSWQGKHILKGKTPTANSLIFSSNDYLSISQHPQLIKAQIAAMQKYGNGQMQSAVFLN
                     NSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
                     GVKAARAKPIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFK
                     CLLIVDESHSLGTHGSQGKGMVAKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEY
                     IRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADFLREALSYEGINIG
                     SSGSQIVPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISANHEKSD
                     LLRVIECLAKLARKRPDIPLFKGN"
     gene            16154..16582
                     /locus_tag="ACF2SP_001443"
     CDS             16154..16582
                     /locus_tag="ACF2SP_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFD2359233.1"
                     /translation="MRLNIALILSFFSIMLFAMDEVDEQQILQRIQPVGKVRVQEEQG
                     NNIEAGATKTTVVDSKSKKEPGQDIYDKYCSVCHRDGLAGAPKFQSEADWKPRLASKK
                     IDDLLASAIKGLNAMPAKGTCMDCSDAELKAAIQYMLPKS"
     gene            16579..17070
                     /locus_tag="ACF2SP_001444"
     CDS             16579..17070
                     /locus_tag="ACF2SP_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFD2359234.1"
                     /translation="MKKLTYRKIQSFQAIITVLVIFASFYLEYAVGLQPCPLCLMQRV
                     CVFILLGLMGLSLGTVKKAHIVSLMQFLVACAGLYFSLRQLWLQSLPSDQAPACMPGL
                     DVLIQYFPWQTVAKALFWGAGDCAEVTWTMFGISMPGWAAMYFLFMAIMGLFLFFRTR
                     TIN"
     gene            17132..18283
                     /gene="tgt"
                     /locus_tag="ACF2SP_001445"
     CDS             17132..18283
                     /gene="tgt"
                     /locus_tag="ACF2SP_001445"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFD2359235.1"
                     /translation="MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVN
                     NMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVF
                     SLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
                     KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSA
                     MNTDGIAIGGETVGFDMNTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGID
                     MFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRIQIKKSCFADDLAPVMENCS
                     CYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS"
     gene            18310..18804
                     /locus_tag="ACF2SP_001446"
     CDS             18310..18804
                     /locus_tag="ACF2SP_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD2359236.1"
                     /translation="MVLISTSMGDIHIKLDTENTPLTAENFLNYVRKGFYNDTIFHRV
                     IDGFMIQGGGLKTNMEQKTTASPIQNEAKGAKPNKRGTIAMARTMDPHSATAQFFINV
                     ADNGFLNYSGDHPQGYGYCVFGEVVEGMDVVDAIAKVKTGQRNGHADVPVEEISIIEV
                     RELA"
     gene            complement(19014..>19513)
                     /locus_tag="ACF2SP_001447"
     CDS             complement(19014..>19513)
                     /locus_tag="ACF2SP_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFD2359237.1"
                     /translation="TVRDTLFNTYPLTWVAHPEYLLYTSVCAFLTIFIAHSLVRIMKV
                     FLILDALGLSTFVIIGTQKVLAHGMPPSVAVISGMITGICGGMLRDILCNDIPLVLRK
                     ELYAVIALAGALLFITLDSFHIPHNLNIIITLLCIFTARLLAIFFHIEIPIFDYSESI
                     KRRKK"
BASE COUNT         5811 a         3381 c         3981 g         6340 t
ORIGIN      
        1 tagaactttt tgttttaatc aatatggcgc aatcttatga tgtagatgaa cagttaaaag
       61 ctataatgag ttggctgtat caatttaatc ccatattaaa ggcatcaaat aaagggttat
      121 tatcttttta tacagaattg gaaccaaaat tccactcgga aggtcactta gagaagagag
      181 tagaaactgg gacagatgat ttattgtatc gtattaaaac ttttttagtg tcattatttg
      241 ttaccccatt cgaagttttt cctttttcag gtaaaacaat cagtttttgg gatatcgaaa
      301 atgtaatctt ttcagaagga caagagatct ataatcagtt tgctcctttc ataatgacga
      361 acaagttaga cactttaatt gcagtctata aaaagataat ggatgagcat attatccctt
      421 gccaaaaaaa taaacacatt tgtaaatggc tgacacacta tcaatcaacg ctggattggt
      481 atcagcgggt agaggcgggt gaattatcta aaatggatgt ttattggttt gacccggaat
      541 tattatttca tgttttagta cattttagat tgattaataa atcattgggc gaaaaaatcg
      601 ttaatttttt ggatgaatta atacatacct atgctcaaaa taataatgag tttcaaattc
      661 aactgagagt caatatttta ttttccagat tcttgaagag tttagatgag caacagagac
      721 gaaaattaat tttaactttg tcattgtttg atcctgtaga ggctaaaagt aaatttttaa
      781 cgaactgcat tcattatgtg acaaaccgac tatgtcaaat tagtatgcat cagttagaca
      841 gttcccctaa tttttttggc acttaccaat gcatagatag taaaaaattg ttaattaata
      901 aaacagatgt aaagcaagtg agtgctattt tggaagcctt taaagaaatg cttcattcgt
      961 tagaagagcg ttgtaaccct gagcaattgg aaaatatgct cattttcctg cgaagtataa
     1021 gccgccctat tttgactgtt gcggaaatag aagaggcgca acaatcagcc cgagttatag
     1081 attatatagg cgctccgacc tgacttaatt aactggaatt atcctaattc attattgtgc
     1141 attttgatct catcgcgctg ataaggatcg attgtcttgg cctggatgag caaagcttgc
     1201 tccagtgtat gaattgaatt ataattaacc aatttgtctc atttatgctt ttaaaattat
     1261 aatatgtcaa atctaataga acttgcttta agaaacgcgg cacttgatag aacaaaactt
     1321 ttaagtcagg tacaaaaaat tgaattttta aatggttatg atcccaggtt tgtcgaggga
     1381 ttgatatcat tagaaatgat aggcgctatt gatgaatacg ttgcttcttt gatgtatgtt
     1441 aaaatgactg ataaatacac tcaggaggaa aaagggcaat taacagatac tatattaacc
     1501 atcaataatg atcgattgat ttcatgtcaa tatcgctttc ctcaattata tcaaaaatta
     1561 atgcaagacc aatttttatc tactttcttt tcaagttcgc atttactccc caatattgct
     1621 ccaggcacaa ataatcaatc ttatgtaaca gtaagtacta ataataaaat gaagatggag
     1681 tcagcaaagt tatatgggca ttatgaatat tatcaatgtg ttgtcgcttt aatcactcaa
     1741 attgatggtt ctcgatattt ttatcatttg aatccaaacc gtatctttgg tgattacatg
     1801 ggtagtgcgc ctttgaaatt atcaggtagt gaacttaaag atgtaaagga tataaccttt
     1861 atccttcacc cgcgaagtat ggttgattat cgcgaattgc aaagattgca agagagaaat
     1921 attgttttta aaaccgttat gctaccagtg ggggcaaagt cggtatcact tagttatgat
     1981 acagataaag atgtattgac agttaacaat cttgaggcag gacaaactgt aacatataac
     2041 aatctgctac aaacacccca atataataga tatcaatata cacctgagca atatttcatg
     2101 acccaactat atagaggcca aacatgggca gaagcacttg gcgaggcatt gaataaatat
     2161 attgttacaa gacaagaagg ggcaacctgg tgggatctga aaccattatt ttttaatcgt
     2221 gagactacaa aaaataatat gattgctgct gcgcgaatca aaaaggaagt tcaggctggt
     2281 gctacctcat ttgattttac aggggttagc agaaatggct tgctgaatca attattaaca
     2341 actgctgttg actatcaatt agaataccag cgtgggttgc aaaacgggat gctttcggct
     2401 gaagacctaa caacaagtgc tgcgttacgt tgataactga ttatctattt tcggaagagc
     2461 tttttgttta ctcttttaaa ctattaaatt gggcacataa ttttgtaaaa ttttcatatc
     2521 gttgggttga aatctttccc ttatttagcg cgtcaatgat tgcgcattgg ggcgtatcaa
     2581 tatgtgtgca gttgcggaat ttacattggg acaaataagg tttgaattct ttatatcctt
     2641 gagcaatcat agagcaatca atatcccaca aactgaattc ccgaacgcct ggcgagtcta
     2701 tcaaggcacc accactaggt agatgataat agcgagagtt tcttgtagta tgtttgccta
     2761 actctgaaac catggatatc ttatcgatcg caatattttc ctcatgtggc aataaacttg
     2821 aaatcaatga tgatttgccc acccccgatt ggccaacaaa aacactgatt tgattattta
     2881 atgcttgttt taatggtact attgtgtctg gggtttggtt acttgccatg atgacgggat
     2941 agttcagcaa ttggtaatcc gtcatgagtc gctgctgaag tgtttgacag ggaagatcaa
     3001 ttttattgag aacgatcgcc acgtgtaatt gaagtatctc tgccataatc aaatagctgt
     3061 caagtaatgg ccaactaatc tctggttttg gagcaatcac tacaaccagt tgagtgatat
     3121 ttgccgcaac gggtttcttt aaccccttct gactgggcct tgccagtaag cttcccctgg
     3181 gatagatgct cacaaccacc ccttgtttct caccttctat ctgccagatt actctatcac
     3241 cagctaccag tgtgtcaaga ttaggccgga tagagcaaag aattcgtttc ccctgattgt
     3301 cttcaatttc tgcatgcctg ctaaatcgag taatgactaa accatcagcc aggttatcgg
     3361 agttattttc catgctttca tggtagtttt tctggatttt agctatacgg gcagattgct
     3421 gcttattgat tcgtctttta ctcatagtgt caatctatac tataattgat gcgattaata
     3481 atacagtatg aaggtaaatc atatttaatg aaagtctact tggtaggcgg tgccgttcgt
     3541 gatcgattat taggaattcc tgtccaggaa caggattggg tagtcgtcgg agcaacgccg
     3601 gaagaattat taaaaagaaa atataggcaa gttgggcggg attttcccgt ttttttgcat
     3661 cctgaaacga aagaggaata tgcattagca agaacagagc gtaaatcagc tccaggatat
     3721 tatggattca tttgtgattt tagtgaatcg gtgacgttgg aggaggattt ggcacgacga
     3781 gatttaacga ttaatgccat ggctatggat gaacagggga atttaattga tccatatcag
     3841 ggacagcgag atctcgaaga gaaactatta cgtcatgtgt cccccgcttt tgcagaagat
     3901 cctgttcgag tattacgtgt tgcccgtttt gccagccggt ttcatcatct tggatttaaa
     3961 attgccaatg agactcgttt attaatgtat tccatggtta aacaaggtga attagctcat
     4021 ttgattcctg agcgtgtttg gcaggaatgg caaaaaagtc ttcaagaaaa aaatcctgaa
     4081 caatttattc tttctttacg ttcctgtgat gcattaagag tggttttgcc agaaattaac
     4141 tcattatttg gtgtgcctaa tccgcatcaa taccatcaag aaatagatac gggcattcat
     4201 tcattaatgg cattaagggc atcatctgag cttagcgaag agccattagt acgatttgct
     4261 gctttggttc atgacttagg caaagcctcg acaccaattc aggattggcc taaacatcat
     4321 ggacacgagg aggagggtac caagctgatt cgagctttat gtgctcgctt gcgcattcct
     4381 aatgattatc gcgacttggc cgttacagtg gcgcgagctc atttgaatat tcatagggta
     4441 tgcgaattgc gtcctaacac aatcgtcaag cttttggagc aggtagatgc ctttagacga
     4501 cctcaattat ttcataaaat attgattgct tgtcaagcag atgccgagag ttgtggcaaa
     4561 acagtggtct atcgccaaac tcaattatgg aatgaaatat tatctgaatg tgttaaagtg
     4621 acgccacaaa cttttatcgt acaaggttat gaaggtaaag cgattaagga ggccatgcat
     4681 caaagtcgtg tggcttgtgt agagcgaatt atgacttctt ggaaatcaaa tgaaaaataa
     4741 tcaaaattta atttggattg atttggaaat gactggcctt gagccagaac aagatagaat
     4801 tatagaaatg gccacgattg taacggatcc acaacttaac attctcgcag agggacctgt
     4861 catagcggtg tctcagccta aaattcttct agacagtatg gatgcatgga atacaaagca
     4921 acataatcag tcgggattgg tgaagcgagt tttagagagc aatgtcagtg aatctcaagc
     4981 tgaacaatta acgattgaat ttttaaaaca gtatgtggat aagggaaaat cacccatgtg
     5041 tggcaatagt atctgccagg atagacgttt tttatataaa tacatgcctg agttggctgc
     5101 ctatttccac tatcggaatt tggatgtcag ttcgttgaaa gagctggtat tgcgctggcg
     5161 tccagaatta atgaatggag tagttaagga atcgaaacat cttgctttgg atgatataaa
     5221 agattcaatc aatgagctga tttattatcg acaacatttt attaatttgc cggaagtaaa
     5281 aaatgacaaa tagagaacgt ttagaattac ctaatggcgc taaaaagtta ttacttcatt
     5341 cctgttgtgc tccttgttca ggtgaagtga tggaagcgct cattttttct gagattgact
     5401 ttactatatt tttttataac cccaatattc atcctctcca ggaatatgaa ataagaaaag
     5461 aagagaatat taagtttgcc caaaagcata atattccctt tatcgatgcc gattacgata
     5521 aagaaaactg gtttgctcga gccaaaggat tggaatggga gccagaaaga ggcaagcgtt
     5581 gcaccatgtg ttttgatatg cgatttgaaa gaacagcact ctatgcttat gagcatgact
     5641 ttcctgttat cagtagctct ttgggtattt cacgttggaa agatatgaat cagatcaatg
     5701 attgcggagt gcgggcagca agtcattatc ctgatttggt atattggact tataattggc
     5761 gaaagaatgg cggctctgaa agaatgtacg aaattgccaa aagagaagca ttttacaaac
     5821 aagagtattg tggttgtgtt tattctttac gagatacgaa tgcctggcgc aagcaaaata
     5881 caagagatcg tattaaaatt ggacttaatt attactctgc aagcaagaac gaaggagaat
     5941 tatgagtaag cattcgcatg atggagcata ccatcatgag catggggaag tgacttatga
     6001 cagggctttt ataattgcaa ttactgcgaa tggccttttt gttgtgatgc aaattatttt
     6061 tgcctattta gcgaattcta ccagcttgct agcagatgct tttcataatc taggtgatgt
     6121 attagggttg attttagcat gggtagcaac cggattaatg aagagaaaac ctacccataa
     6181 agcaacatat ggatttaaaa aaacctctat tttagcggca ttggccaatg gtgcgttact
     6241 ggtatttact tgcgggatca tagcgacaga tgctatgtat aagttatttt caccgactga
     6301 agttcaagct gtctctgtga tgatagtcgc cgcgattggt atagtcgtga acttctctac
     6361 agcgctcttg tttttacggg gctccgatga tctcaatatt cgaggagctt atttacattt
     6421 attttatgat gccttgatct cggtaggtgt tgttttatca gcgggtgtat tatatctgac
     6481 gggttggtta tggatagatc cggttgtcgg actattaatt gcattagtga ttttgaaggg
     6541 cacttggtca ctatttgctg atagtttcag attaattatt gatggtgttc cccgtgatat
     6601 atcctggatt aaagtcagtg attttctctt ggctatacct ggcgttaaat ctattcatga
     6661 tttacatatt tgggcaatca gtacgaaaga aaatgcactt tctgttcatt tatttatgcc
     6721 agaggagtca ttatctgatg atttgcgtag agagctggtt gaaaaattaa agcatgattt
     6781 cagtattcaa catgtcacta tccaagttga aaagacagaa gcagaatgca atgacgcatg
     6841 tcataatccc aggataatca tttaattggg gctggattta cgacccctaa tttctttgtt
     6901 taaaaaaggg tttaatttat tcattaattg tatctaagcg cccccattaa aaacattttc
     6961 gtcttgcatt tcgagttatc tttttcagtt ttttaatcaa ttaggataat tcatcttttg
     7021 ttatcaactc cagacctttt ttataacaag agccactgga taagggatca tttagctttt
     7081 catggagttt accaagttct ataaatgcta cgggcgtggg atttagctca atagactgtt
     7141 caagataatg tttggctttt ccccataaat gccgagagag gcacattctc cctaaacata
     7201 ggtacaatgc ggctgagtga ggattttttt tgagtaatga ttcagcaaac aacaattgat
     7261 tttcttcaca agggagcagg ctataaagct caattaaccg attatccaac tctttgcgaa
     7321 ggcatcgcct taaatgatat tctgcctgat cgtaatcctg attcctaatc agaaagcggg
     7381 catattctgc tgtaatcgct gtatcgttaa ccattgtttt tggcagcgtc tgatacaatt
     7441 tggtaatgcc atctgattga ttgtgttttg ccaaatcaat catggcttgc aaataagtat
     7501 ttttttgtaa ttgttcaaaa gcttgatctg aaataatgtg gttctttttt aaatcaggta
     7561 aaagagcgat caactgctgc caatctttta tttcttcata aagattcatt aataatttca
     7621 agacataagg atggcgtggt gctaaatctt gaagatgctt taatgtggcc aatgcctgtt
     7681 cccattggtg attggccaat tgtaattgcg cctgggttaa ttcaacagca attttcgctt
     7741 cgggcataga ttgctgtgct tccctaagat aatgatcgcg aagcttgcta tcccccattt
     7801 tttgagcagc tctcgctgct gttaaataat taagtaaagg agtgtcggtg tttggcagag
     7861 cttgtattaa atgatttttt gctttttgcc aatagccctc gctatattct atcagtccct
     7921 ttcgagtcgt ggcttgagct ttttgtgcca agcgccgtga gtgccattta ttgaccatgc
     7981 ctggcattct tgtaatttta ttgcaaagct gaaccaggcc atagattaaa aaaaacagaa
     8041 aaaacaaacc aaaagcagag acccataagg tggtttctat agtccagtta ttaatagcta
     8101 ttaagacata acctggatcc ttattgagtt gaataccaag gaacacagag agcagtaaaa
     8161 taataaaagc aaacagaact cgtatcattc ttagttttct cctttcttgc tgtttgcttg
     8221 atcgaccagc aattctttat tgtcaatcat ttgattgatt agcgggatgg cttgtcctac
     8281 gacaggtttt tcctgagtta attttacatt ttgcagggtg gataattgct taatcaaagc
     8341 gtcagtgttt tgggaagatt cattaaatac tcttttaaga tttgcaatgg cctggtttaa
     8401 agcaaactgg tagactgcgg ggttattatt taaaatagcc cactgcgctt cctgaaggtt
     8461 aaggcggata ttctctttta ttgcagattc atatagaggg gagataagtg gttgaatatt
     8521 ttcattgtgg cgacgaataa caactaattt ctccagaaaa ttcacactgt cttgcaaccg
     8581 atttcgccat ccagttttac cagactcttc agacttggtg atatggttta gcacttcctg
     8641 ttgatctact actgattgaa tagaaagatt attgatagcc gcttgagccg catccaattg
     8701 gcttaatatc cctgtgatat caacaacaga aattgacttt aattgggcaa tttctttggc
     8761 gattgtctgg cgaatggtaa atattttggg gatattcatg cctttcagta gggcatcagc
     8821 ttgctgtaaa agggcgacag aggtattaaa attatcactc caatgaacat tgatttgtgc
     8881 taattcaaga taataacgtg cttttaaaag aagccaatca ttattttgat acaatttttg
     8941 cttcatagct gtttgtaatt ctttatttaa gctatccatt ttgcttgtta aatcactctg
     9001 aatttgctga agatgattgg cattgttatc caggagcttt tggactgtat tttgctctgc
     9061 ttttattctt cccaattcat cagttaactt tttgttttca acaagctgat ggttttgaaa
     9121 ttgtttgtcc aattgaatgg aataaataga gatgatgata gcgaggaacg ccaaaagcga
     9181 tgtcaagctg attattaagg ataggtattt actaacgaag aaaggggctt tcatttgttg
     9241 gttgatgtcg ggctcaactt tagatgattg ttgcggctga attgtttttt tctgttcttc
     9301 attgctatcg gccatggatt aatccttata gtcaaataac gtacctatca tcctttctgg
     9361 atggctaata agaatctttt taattccaaa taaagaagct acctttgcaa gtcggtcact
     9421 aatgaccagg caaggtttgt tttgtaacca catttgcccc tctatgctaa acattttaaa
     9481 aagattatgt atagactgct cacttgttaa cagtataatg tccgccaggt catcgcgcca
     9541 cagagaatta agaaattctt ggtcgatggt tggcatgact cgtttataga ctgacagaag
     9601 acatagcttc gctcccttca gcattaaata ttcttcaatc agttgcctgc ctccttctcc
     9661 tttcacaagc agaatcgtct gatttcttat ttgttgcaac gaattgagag tcagtaaatg
     9721 ctcgctgtca ggtaattcgg ggacatcact aacctggata ttgaattcat tgagtactct
     9781 ggaggtgcct ttacctatag cagctacccg gatgctattt agccaattga tgtttttgga
     9841 agccagttcc atcatgaaat aacgaactgc atttgcactg acaaaaatgg cctgatttac
     9901 tgaggtcaaa tcgggcaaat tatttatcca attgttctca gatgccatta tttccatcgt
     9961 agggcagtcg atgggtattc cgccagactc gataattttt ttattaagta cttgtgcttg
    10021 ctccctgggt ctggtgttta atatgcgaag accattaagt gatttggtca tgatggtatc
    10081 gtacttaata aggacattgc acctttagtc attaatgact cggtacacct gtctgcaata
    10141 atttcagctt cttctatttt cccttgttgc tcatcatcaa tcatttgaga accatccaga
    10201 ttaagtactt ttgctctcaa aaggaggagt ttctccgcag taatggtaca ataaacagca
    10261 aagggtatat ggcaattgcc tcctaatttt gcgttgacgc gtcgctcggt atgaacacaa
    10321 agggcagaaa ttggatcatt taagccatga atcagctcct ggatttccaa atcgtcagtt
    10381 cggcactcaa tacataaagc gccctgagca caagtaggca gcataatgtc gtcgggtatg
    10441 agttgggtga tgtgatgtgc caagcccatt ctttccaagc ctgcggcagc aagaataatg
    10501 gcttgatact ctcctgactc cagtttgctg agtcttgtat ggatgtttcc tcttaatgtt
    10561 ttgatttgca aattgggatt gtaagccaat agttgagatt gacgtctaag acttgatgta
    10621 ccaataattg cgtttttggg gagagcatcc agactcttaa atcggggact tattaaagcg
    10681 tcaaaagggt tatctctttt gcaaatggcg gccaacgaga ggccatccgg aagttgtgct
    10741 ggcatgtctt tggtcgaatg aacagccaag tccgctcttt tatccaataa tgcttcttct
    10801 aactctttaa caaataaccc ttttcctcct gcggataata atttatcttt aagaaaccga
    10861 tcgccagagg ttatcatggg taataattca atgcttagat ttggccattg ctttacaagc
    10921 atttcccgta catgattagc ctgccatagt gcaagcgggc tctgcctcgt tgcgattcgt
    10981 ataatttttg cgctcatgga atagacggta tatgttcttt aggtaaagat cataacattt
    11041 attctaattt aaaaagaacc ccattgagat aataggtacg caaatcttgt cgggcaattg
    11101 atttataaat gagcattcct accggtttga gaatgggttt atagtgccaa cccccaattt
    11161 ctttgctaaa aaatttttta atcctgtcat ttatagtcat ttaacaatca aataactata
    11221 gcttatctaa gctccttatt aaaatttttt ttgcctcgac cttggcgctt tgtgtaagtc
    11281 ctatagagca tatctcatgc acaatcccta tattttatga taggctttcc cggtagataa
    11341 agcgctgaaa atcaggggcg ttttttagct ggatttaagg ataaggaatg tcacaactaa
    11401 aaaaaatagt gaaacattta gatgaatcaa tgcagcgtag tttgtctaat tctgctcatc
    11461 aattggttgc agtaggcgcg atagcttttg tgggatttcc tttattttat gttatttggg
    11521 ctttctggtt gccccagcct tatgaaaacc tgccactccg tcttattgga agccttttag
    11581 gtttgggatt gatgcttact ccttattggc cattaaaatg gaagcaatat ttatcttggt
    11641 attggttttt aactcttttg tttactttgc catatttttt tactttttta tttttaatga
    11701 atcaagcaag cgttatttct gcgatgtctt tgttgtgtgg tgtattttta cttgtattgc
    11761 tcgttgattt attgagttta tccattgtgc tgatattggg gtttagttta gcccttgtaa
    11821 gttattatct tgtttctccg caaatgtatt ttggagaaga gcatatacaa atgacattgg
    11881 tgatcatatt taccattatt gcaggttcta ctttaaatta taaaacagcc atgttgcaac
    11941 aacaaaaatt ggctggtatg gctgctgctg caggcatgat tgcccatgaa ttgcgttcac
    12001 cattgttagg aataaagagc ggcgctcaag ctttagtcca atatttgcca agattgattg
    12061 atggttatca attggctaaa aaacaagagt taatatcctc acctttacgt gacagattgt
    12121 tattacagct tgggggagtc agcaatcgaa ttgttagtga aattgattat gctaatacga
    12181 tcattgatat gttattagtc aaggcaagtc gcgataatat tttgcaaaat tgcatattag
    12241 agacttgttc tatagcagaa tgtttgtctg aggctattac cagataccca tttcagtcac
    12301 caagagaaag aacattaatc acatggactg gtgattttca atttaaaggc tctaagttgt
    12361 taatgcagca tattttattt aatttaataa aaaatgcact ttatgttatc gcaatggcgc
    12421 aacgtgggga gattaatatt tggactgaat ctggcgataa gtatcattat ttgtatttta
    12481 aagacacagc aaaagggatg agcaaacaac aattaaatcg attatttaat catttctata
    12541 ctactacctt tatgggaaca ggcattggtt tatcattttg taaattagtt atgaatgcgt
    12601 ttggtggtga tattgtttgt gaatctaaag agggcgaata cgctttgttt atactgagtt
    12661 ttccaggctc ggtttaaaat tggttttctg ttgaacatac catcattcgt cttaaaagac
    12721 gtaaggatgg tattgagtta gagagcccct gtgattctct tctgctagag tttggccatt
    12781 tttactgaga tagcgataaa aatatatgtc caaccagtga cgatcaattc aattgcaata
    12841 aaaagcccaa tgatccacag cccgctaact ggccattgca ttagaattaa tatcccaaga
    12901 ataatggcgg tgattccggc caataacaac catccccatc ctttggagtc ttttaaggca
    12961 aaagccataa tcattcttgt taaaccaatt acaataagca ccgctgccag aaaagctgtg
    13021 attagagtag atgctaaaaa cgggtcatac aaaacaatgc atcccgccgc aatatacaac
    13081 actgcaataa gcgcctgcca tatcattcct ttccactctt tgtatttaaa tacgtctata
    13141 atgtgactta ttcccccaat aattaataag gcaccaaaaa agaacatact taccaaggtt
    13201 agaccaacta ccattccaag accaacacag ccaaggataa ggaaaaatat tccaaatcct
    13261 aaaatccatc cccagtttcg ttttaactca tccactgggg gttggtaaaa ttcttgtgaa
    13321 ttagccatat caatatcctt ttttgatttc ttagctaaga taaggtactt attaataata
    13381 tagacttata ttcaataact tgtctaattt tgagctttga ttttaagaac tcttattttg
    13441 aatactcact ccaaaaagcc cggtgaatat aaaacgtaaa taatttggtt ataataaaca
    13501 atcatgttac tttattcaca agttagccag gttctgatga tggagccata ggttatttga
    13561 agttcataga aaggatataa ctttggaacg ttttgctcag gaggagccat gcaacatttc
    13621 tcaataccta cctgttactt tccaagcact gcggtttttg tcgacgacag tcgggatttc
    13681 ttgctcaatt tcgtattgca gctggatgag ggtttggcgt accgtgtatt tgattcccct
    13741 tttgaagctt tggattgtat taaacaaaaa cgctgtgaac tggaattatt aagtgaacgt
    13801 tgtctcagcg aatacacaga agcaaaaaat tgcccactaa ctaatcatac cattaatctt
    13861 gatttggctg ctattcatgc cgaggtttat aactcccgac gattttctga aatttcagta
    13921 gtcgttgttg attatgcaat gccaggcatg gatggattag agttttgcag gcgaatcgaa
    13981 gatactaata ttaaaaaaat attattaact gggcaagcag atgagaagct ggcaatcgct
    14041 gcctttaatg aaggattaat tcatagatac atcaaaaaga gtgatccgga tgtcgccagt
    14101 ctcattactc aaagtatcca tgatttgcag ttacaatatt ttcaaagcat gtctgatatg
    14161 attgtacgta tgctttcggt gacatcccca aattgtttac atgacaaaaa atatgctgag
    14221 ttattctggc gtttatgcag ggaaaaggga atcgttgaat tttatctggc cgataactca
    14281 ggtagctttt tgatgctgga tgatgatgcc aacatcagtt ttttaatcgt gaaaaacgaa
    14341 gcggatatgc agttacacta tgatttggct ttggataatg gagctagtgg agatgtattg
    14401 gaccaattgc ataacggaga aaaaattcct tgtttttggc aatctaattt agtgacacct
    14461 caatggaatg agtggtcgaa ttgtttggtt cctgccaacc gatttgtttc tgatgaaacc
    14521 tatttttatg cttatgtgca aggagctgtt ttgtttgatg tgcgcctgga taaaattctt
    14581 tcttatcacc aatacctgga agagctggat gcagaagaaa tgtttctgaa ttaataggaa
    14641 ttctatataa agagcatata attcttattt gaaatggtaa ctgttcaaat gagctgccaa
    14701 taatagaagt cctgttaatt acccttaaac aaaggaatat cgggtctttt tcttgcaagt
    14761 ttggctaggc actcaatcac tctgagcaag tccgattttt cgtgattcgc actgatggat
    14821 aaacgaatca ggcatttatt tttgggagtg gcgggtgaac agaacacggc tccaaagagg
    14881 tcttcttttt ctaactcatc ccttaaccaa agcgtgttag cctcgctgcc tgtaactaaa
    14941 gggactattt gagagccact acttcctata ttaattcctt cgtatgataa ggcttctcgt
    15001 aaaaaatcag cattggtttg taattttgct cttctccatt cttcttgagt aataatttct
    15061 aatgatgcgc taaatcctgc taaatcatga ggcattaaag cagaactaaa aatagaaggc
    15121 agagatgaat atctgatata ttctataagt tgcctatctc ctgtaatgat gcctccacgt
    15181 cctgataagg ctttagccag gcttgctgtt atgatgtcta tttggcttga taatcccagt
    15241 ttagccacca ttcccttgcc ttgcgagcca tgcgtaccta atgaatgaga ttcatcgacg
    15301 atgagtaaac atttgaattt tcttgcaatg tcagcatagt cagccaatgg actaatcgta
    15361 cccagcgtac tataaataga atctactgca attattcctg gtccatggtg ttcaattcgt
    15421 ttttttagtg agtcgactga attatgtaga aaaggtatag gtttggctct tgctgctttt
    15481 acaccagacc aaaaagacat atgagcataa aaatccagat acactggtgt attgtacttg
    15541 gccagggttt gtatgagacc aacgttagcg ctccagcctg attgagtgag cagggccgca
    15601 gaataattta aaaaggaact gaatttcttt tcacattggc tgagtaaaaa agagctgtta
    15661 tttaaaaata cagcggattg catttgacca ttaccatatt tttgcatcgc agcaatttgc
    15721 gctttaatta attgcggatg ttgactgata cttaaataat cattgctcga aaaaatcagt
    15781 gaatttgcag tcggggtttt gcctttaagt atgtgtttcc cttgccagga tttattgact
    15841 cgcccctcgt aatatttttc catggaatga ttgatgtact caggataagc atggttttga
    15901 tgtttttctt ttccattgat gtattgagtc tcgttatcaa ttgtttggta ttctgtattg
    15961 ctcatccata actcagattc ttttcccttg tgtgcagttt agcacaatgg ttgatagtgg
    16021 aaggatcttt tcagtaaaag tctaagtatt aagttaatga ttttataaat cgttgggctt
    16081 attgaaatct ttctggaaaa aaaagtcact tgtcggtatg atttttaaat taactattag
    16141 aggacggata aatatgaggt tgaatattgc gctgatcttg tctttttttt caatcatgct
    16201 ttttgcaatg gacgaggtgg atgagcaaca aattttacaa agaattcaac cggttgggaa
    16261 agttcgagtt caagaggaac aggggaataa catagaagct ggagcgacta aaacgactgt
    16321 tgttgattcc aagagcaaaa aagaaccagg ccaagatata tatgataaat attgctccgt
    16381 atgtcatagg gatggattgg caggggcgcc taaatttcaa tcggaggccg attggaaacc
    16441 tcgattggca agtaaaaaaa tagatgattt actcgcttct gcaatcaagg ggttaaatgc
    16501 catgccagca aaaggtactt gtatggattg ttctgatgct gaattaaaag ccgcaataca
    16561 atatatgttg ccgaaatcat gaaaaagctt acttacagaa aaatacaatc ttttcaggca
    16621 attattacag tattggtaat attcgcttcg ttttatcttg aatatgctgt tgggctacaa
    16681 ccatgtcctt tgtgcttgat gcaacgagtt tgtgtattta ttttgttagg attaatgggg
    16741 ttgtctctgg gaaccgtgaa aaaagcgcat atagtaagct tgatgcagtt tctggttgcg
    16801 tgtgccggtt tgtatttctc tttaaggcaa ttgtggttgc aatctttacc atcagatcaa
    16861 gctcctgctt gcatgcctgg gcttgatgta ttaattcagt attttccatg gcaaactgtt
    16921 gctaaagccc tattttgggg agcaggggat tgtgctgaag tgacttggac tatgtttgga
    16981 atttctatgc ctgggtgggc tgccatgtat tttctgttta tggcgataat ggggcttttt
    17041 ctgttttttc gaactcgaac gatcaattaa ttcctggcat taaacgcgtg ctttcgttat
    17101 cattctttgt tttatcttgt cgtttctgcg aatgaacact ttttcctgtg aagtactgta
    17161 ccagcattcg ggtaccaaag ctcgtgttac acgtgtggtt acgcctcatg gtgaatttat
    17221 aaccccggta tttatgccag ttggaacccg tgcaggggtc aataacatga tgccgtcaga
    17281 actgtctgct gcgggtactc aaattattct gggaggtaat acctatcata tgctgtgtgc
    17341 gcctggcatg aaggtgatag aaaatgcagg ggggatgcat cgctttatgg ggtggcatgg
    17401 acctatgcta acagacagcg gtggttttca agtttttagt ttatcgaaga acaaggaaat
    17461 ttgttctata gatgaatctg gtgctcattt taaactgcct ggcagtaagg ggattatcca
    17521 tatgaccccg gaaatgtctc tggagaccca gaaaataatt ggtgccgata ttataatggc
    17581 atttgatcag tgtactcctg acagctgctc caaggatgaa gtaagtcaaa tcatgttgcg
    17641 gacccacaga tggctaaaac aatcgattga ctaccatcaa caatatccca actctcgata
    17701 cggatataaa caagctttat ttggcattgt gcaaggagga gtttttcaag atttacgtcg
    17761 agaaagtgct gattttgttt ctgcaatgaa tacggatggt attgcaatag gtggagaaac
    17821 tgtaggtttt gatatgaata ctacagtgga agtcattcgc tgggtgtatg agtatcttcc
    17881 gggcaataag ccacgttata ctatgggagt tggaatgtca cctcaagatt tgttggatgt
    17941 ggtggcagaa ggtatcgata tgtttgattg tgttgcacca acacgtaatg cccggcatgg
    18001 tgccttatat tgtggtaaat tggtaagaga agggaattgg ctaaaatttc aaagcgagca
    18061 tgaaaatgga cgaattcaaa taaagaaatc atgttttgct gatgatttgg cacctgttat
    18121 ggagaattgt tcttgttata cttgtcttaa ttattcaaga tcctatttgc atcatttatc
    18181 aaaacaaaaa gctaatttat ttactgcttt ggcaagcatc cataatgttc atgttttgca
    18241 tgatgtttgt aatcacatgc gtcatttgat aataaattcc taaattattt aatattaaga
    18301 ggtgaaataa tggttttaat ttctacatcc atgggtgata ttcatatcaa attagataca
    18361 gaaaataccc cattaactgc agaaaacttc ctaaattatg tgcgtaaagg tttttataat
    18421 gacacgattt tccatcgtgt tatcgatggt tttatgattc aaggcggcgg tttgaagaca
    18481 aatatggagc aaaaaacaac tgcctctcca attcaaaatg aagcaaaagg agcgaaacct
    18541 aacaaacgtg gaaccatcgc aatggcgaga acaatggatc cccattctgc aacagcccaa
    18601 ttttttatta atgttgcgga taatggtttt ctgaattaca gcggcgacca ccctcaaggt
    18661 tacggttact gcgtatttgg agaagtagtt gaagggatgg atgtggttga tgccattgct
    18721 aaagtgaaaa caggccaacg aaatggtcat gcagatgtcc ctgtagaaga aataagcatc
    18781 attgaggtta gagaattggc ttagcgagaa aatatatggc tgaaatagca taggttttcc
    18841 atagcaaagg tatattttct ttaatttcat ccccagtatg gaagagggat cttccttatg
    18901 catgcaggaa gccgcatttg caaaacagcg tgccattttt cagaggcaaa atatcatgcg
    18961 atatctctaa gagattaaca tcattattta agtgttttaa tgaaaaaata acgttatttt
    19021 tttctcctct ttatactctc tgagtaatca aaaattggaa tttcaatatg aaaaaaaata
    19081 gccaataagc gagcggtaaa aatgcataac aaagtgataa tgatatttaa attatgaggg
    19141 atatgaaaag aatctaatgt tataaacaga agcgcaccag ccagggcaat aacagcatat
    19201 aattcttttc ttaaaaccag aggaatgtca ttacataaaa tatctcgcaa cattcccccg
    19261 caaatcccgg taatcattcc actgattact gcaacacttg gtggcatacc atgagcaaga
    19321 actttttgtg taccaataat aacaaaagtt gagagaccaa gggcatcaag aattaagaat
    19381 actttcatta ttctaaccag tgaatgagca ataaaaatag ttagaaaagc gcatactgag
    19441 gtatataaaa gatattccgg gtgagctacc caggtcagtg gataagtgtt aaataatgta
    19501 tcccttacgg tcc
//
LOCUS       DAXLDT010000051        19354 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXLDT010000051 DAXLDT010000000
VERSION     DAXLDT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19354
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.051"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            223..660
                     /locus_tag="ACF2SP_001448"
     CDS             223..660
                     /locus_tag="ACF2SP_001448"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFD2359238.1"
                     /translation="MIDDSKAKPQVKLVRGQLQIIASRNIEHRRQLLSDWYKQKAFEV
                     FSKRLDQLLSTAPWIKECPSINLRAMRSRWGSCAVNGKITLNTHLIKAPIACIDYVIL
                     HELCHHFEHNHSDKFYRLLNQVSPNWKQVKQYLDKAANLYLMT"
     gene            complement(650..1324)
                     /locus_tag="ACF2SP_001449"
     CDS             complement(650..1324)
                     /locus_tag="ACF2SP_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFD2359239.1"
                     /translation="MNIKKEIGKRILEARKAKGLTLKALGELAGGLKQTRLTNWEQGV
                     RTPGPEEIKSLARALDVSPAYLMCLSDELQFKEAKNPSQLIPLLDYRQACEASLHTGA
                     ESSGDKVFISVSTSLQPELSTDAFALKITDDSMMPEIRINDVLVIEPSVLPEPGDFVA
                     VKINGKPETIICQYKKLSYTSSEFELLTLNDNWPNIKVSDDINVKIIGVLVQNLRCYK
                     SPKVKS"
     gene            1486..2355
                     /locus_tag="ACF2SP_001450"
     CDS             1486..2355
                     /locus_tag="ACF2SP_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359240.1"
                     /translation="MMDFVINGDELEAMSGLPHFQQLTYLRGIRPYMDVKTGIVGIKR
                     RISHQSISEQLYVEPHQGIKSQNFSRDQVRRSVSGLVRAGLIEVQSEGMHLILKCLLA
                     SSHYSAQNKAAINPPQKATIKPRGESLENTGLTEFEALKADTVESQKAAIPHKDNYYI
                     YLLSQFEKFWSLYPEKKSKNNAQAVFEELKPNAELCHTIINALTAQINYRDTMKLQGT
                     WVPPWKYPANWLAQRCWEDELPTDALQEKNHAEHTKNTRKRHATEDLFCPPCDVKDRE
                     HNNVIQLQRYLQS"
     gene            2321..2683
                     /locus_tag="ACF2SP_001451"
     CDS             2321..2683
                     /locus_tag="ACF2SP_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HFD2359241.1"
                     /translation="MSSSSKDTFKAERRIDLLFSKFAAFYGHVWRSQFKDEVFLKFAK
                     KEWQEALADFTDMVLTRAILNCREFYELPPTLPQLLYCCRQIRKQESFYVVKDVYQPA
                     NKAVVSSCLQKCKELLAK"
     gene            2696..2899
                     /locus_tag="ACF2SP_001452"
     CDS             2696..2899
                     /locus_tag="ACF2SP_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HFD2359242.1"
                     /translation="MLVLTRKAGQQILIGKGLIQMKVLKVENDIISIGIKAPSHIDID
                     REEIYFRKLRQEQEAANDAEMAI"
     gene            2896..3198
                     /locus_tag="ACF2SP_001453"
     CDS             2896..3198
                     /locus_tag="ACF2SP_001453"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFD2359243.1"
                     /translation="MKTDKQLSKRAFQKIRTLYWGTVCLCAPSVSSAQNIESIINRTI
                     NYLQGGLARTVGVFCIIIAGYLCLARQKFPKEYFVMILVGMGIIFGGSSLYSTLIG"
     gene            3208..3489
                     /locus_tag="ACF2SP_001454"
     CDS             3208..3489
                     /locus_tag="ACF2SP_001454"
                     /inference="COORDINATES: protein motif:HMM:NF016957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFD2359244.1"
                     /translation="MSDLEISPIFNALTRPAMTAGVTFEYHMLNLIVSMCAFIGFSPL
                     YGIIFIPLHVFGWLVCRYDTHFFTICAKRFLLPQAPNTSLWRVRAYEPF"
     gene            3476..5956
                     /locus_tag="ACF2SP_001455"
     CDS             3476..5956
                     /locus_tag="ACF2SP_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HFD2359245.1"
                     /translation="MNLSKTLKHVYQEAKVSNLFPVTHLNSPCIFESQSGLVGSVIKV
                     NGAAFEIEEAETLNHQRFLLHQALVSLDSRFIIYVTTHRQKISCPLIGEFKPGFAKDL
                     DERYQQRFKDKNLYKNTLYITVVLKGDDTSKTGSWLQWAKSLSMKGNSELAEHHREQN
                     IQILNRAVEQLHTNLNSFGASILGMQDEELGFSELLQFLGLVVNAGQTASFKHPVYSP
                     PIANSIPQTFKQEAKYPEGHLGQYLSSCQLLFGECIQFQGNTQQDCLFGAMLSLKKYP
                     TNTASILLDSMLSLDCEFIATHTFAPIGRDSALDTISKKRSKLLNAEDKALSQTTALT
                     DLEDGIASETLLLGAHHHTVMLLAPNKSLLDAAILEATKRYGATGVVVVKETLGQEPA
                     FWSQLPCNQHFITRASLITSQNFVDFCPLHNTQTSFSNENFLGGAVTLLETPSKTPVY
                     FNYHARGSKTNPSKGHAAIFGGNNAGKTTLVNFLDAQMGRFGGRSFFIDRDESSKIYI
                     LASGNSSYIKIEPSNPIAMNPLQLPDTSENRSFLKSWFATLVQEDSERAIPSPIAEII
                     NDCIDYSFEQLAPEFRTLSHLSQFLPVDFPRWPHLKRWLKRNDSRIDGEFHWLFDNQH
                     DALNLDFDKVGFDVTYLMDQVQSIIATPVYLYLLHRMRQCLDGRLTSFIIAEAWQLFA
                     SPFWEKALREWLPTIRKKNGHFIFDTQSPKTITDSPIKHIVLDNLATLIAFPNPLADK
                     ETYMEHLKLTEAQFEAIKENTPESRIFLYKQDHEAFLCKLDLSGLSQYIRVLSANTQS
                     VKLLDDIMQEVGHDPELWLPVFFERSKK"
     gene            5953..6663
                     /locus_tag="ACF2SP_001456"
     CDS             5953..6663
                     /locus_tag="ACF2SP_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFD2359246.1"
                     /translation="MKRTACFLIAVLGISGASHADILGVEDAQLIVLAMKQLGELQEQ
                     YRVLTDTYQTTRSQLDNLNQLKSMNSGHYGFGDFNNGLDTLQSWQSPVSTWQDALENL
                     SGGNQQRYQALMDAYEKNHPSLDESSYARFTTPANVARFKEDKAVNRAVLVQTTESYN
                     EINKHMEALHKLSQQIEKTPNTKGAIDLNSRLITEIGFIQLMSLRLQALISQQAAQEN
                     LSALQDRAEMAKFNRLPK"
     gene            6678..6824
                     /gene="lvhB7"
                     /locus_tag="ACF2SP_001457"
     CDS             6678..6824
                     /gene="lvhB7"
                     /locus_tag="ACF2SP_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS-associated protein LvhB7"
                     /protein_id="HFD2359247.1"
                     /translation="MKWLLSVLMIALLSGCSKPPDLDSPCHHFGQYCPQYPINDEPIM
                     ELTK"
     gene            6821..7216
                     /gene="lvrD"
                     /locus_tag="ACF2SP_001458"
     CDS             6821..7216
                     /gene="lvrD"
                     /locus_tag="ACF2SP_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS-associated protein LvrD"
                     /protein_id="HFD2359248.1"
                     /translation="MNTLIKGLMVILIAATISACHHENPLKTQPAKASVRFLINASAN
                     AEKQLHFAIHKDAYGFAYLECMEGKQSPDIQCNALYQGMIAFAKEGHYAGFQMITQAD
                     LTDKKVFESLADEYAETAATSFPHFVSGS"
     gene            7220..8260
                     /locus_tag="ACF2SP_001459"
     CDS             7220..8260
                     /locus_tag="ACF2SP_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFD2359249.1"
                     /translation="MNTLQFDNFIIQLAGEVDKLTNHFVFDGYTALATLLKAPLGAMI
                     VLYIILKGYGIALGVIERPQHELFRFSMRAGLIYMMAMNWDLFSSYMRDLFVSGSESI
                     ATRLMQAVHKYPSGGSINQGLQNVLNEILNLGSDLFDAGSLRKLTPYFAGMMVFLSGS
                     VTIGLAFIEIVIAKLMLAVTLCTAPLFILFTLFDQTKSFFDRWLGVLAGFAFVLIFVS
                     SVVGLCIHLLHWVTYAFLGNTDELTAAIWVPIFIVACLCVMGITQAVAIGKSIGGSVC
                     TSAGSAMVGGFIGGSIGAWGFSKSSVQKPVNTLRQGLGNASRLMEKGKQLGEASHNVF
                     KQLHKNLRRGAS"
     gene            8257..8973
                     /locus_tag="ACF2SP_001460"
     CDS             8257..8973
                     /locus_tag="ACF2SP_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFD2359250.1"
                     /translation="MSKTNLNEYFKRARSWADDQFGRIEQSRNRYQAAFFSAMGLNVA
                     AIMVIGMLAHYQTVVPMLVHHYDNGVTTVEPIDNKETPINRAQIESDIARYIQYRESY
                     DVSSYRAQFELVHLLSNSTVAKEYLQEQDAANTASPIHALGNHIKREVRIYSINFLDS
                     VLANEKDLHKDHHALAEVVFSLIDTDKTSGKTTSTHYNAMISWRYTNPPDSPETRWKN
                     WDGFEVTRYTRQTRLMEAQA"
     gene            8970..9722
                     /gene="virB9"
                     /locus_tag="ACF2SP_001461"
     CDS             8970..9722
                     /gene="virB9"
                     /locus_tag="ACF2SP_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HFD2359251.1"
                     /translation="MKKILLSAAVLLLSLGTYAQNNPTSLSTDTRIKKVVYRENDVIP
                     VHGIPFTTTQIQFAKQERVLDIEGGDTTAWMVTYHPQLENMIFVKPTMFDSKTNMTVI
                     TNQRAYYFSLTSTKKLEQEPGKKTYALKFEYPQAQPIHTKNTTPNKTIHPKVVNTAYR
                     FSGSPQLVPRHVFDDGKFTYFELSSQGSVPAIFAVDDQSGKESTVNTRREGKYIVVQR
                     LAPQFTLRQGGLTASVFNAPEINRIKANRRPK"
     gene            9719..10810
                     /locus_tag="ACF2SP_001462"
     CDS             9719..10810
                     /locus_tag="ACF2SP_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFD2359252.1"
                     /translation="MSMDDKKREDSLGNRTQVAKNPKHNAMKDKLLLAGVVIICIVIG
                     IGGLLPKRKANSVNEPDEKQSLSMALNQNLELIAALKEKNATAQTGYKGSDPRHPPVL
                     KTIQSQTVSKETLARMNAPSTFFNSGGNEVISTATGGEVQASKTLTGRDGNSEFLNQQ
                     NDITSVSAKRLPHPAMTVPAGEMIPATLETAINSELAGMVRAITTRDIFALEGSKLLI
                     PKGSTLVGQFNTAITQGQSRIFVVWNRLQMTNGVIVTLNSPSSDPIGRSGQAADYIDR
                     HFFERFGTGALLSVLGAYTATGGVNGQDEYNSRSQYRMNIASSFQQAANQTLQQDMQT
                     RSTLQINQGAAINVFVAHDLDFYRVAGRA"
     gene            10812..11816
                     /gene="virB11"
                     /locus_tag="ACF2SP_001463"
     CDS             10812..11816
                     /gene="virB11"
                     /locus_tag="ACF2SP_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFD2359253.1"
                     /translation="MEAIKACFSPGATGLLSPLQDFLEDEHVSEILLNKPQEVFIEQH
                     GQLTRFDIPVLTSQYLRRLFLLIANENKQTLSESTPVLSGNLADGSRVQLVMPPASLH
                     ETLSIRKFTLKQVSFEDYKAKGFFSSARGVGVKEYSECIGKTDGLLELYDAKSWQEFI
                     RQAILQKKNIIISGGTSSGKTTFLNSCVGQIPLHERLITLEDTYEMDVPHSNIVRLKA
                     LKQIGDHAQKITMQDLVQATLRLRPDRIIMGEIRGRELFDFVSACSTGHSGALATIHA
                     NNPKVAFMRMAQLYKLNQVAGMDETDIYKVLHEVIDVIVQLQKTTDGRRLVEVYYKHA
                     "
     gene            11809..13710
                     /locus_tag="ACF2SP_001464"
     CDS             11809..13710
                     /locus_tag="ACF2SP_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFD2359254.1"
                     /translation="MRNPWVVAITLIGGLAINGQMAVLLTGIAQWASPLVLVDLLTHG
                     FYWETAIAGCITALLMGCAVGFLKQGFYRYLSFLISSILLGGMVVFLLGFELYSWIFL
                     SSVQSLSGLMELAFLRYQEPELWHLFLTVEGIVFILTFLAIALYLFHWLKPNNKALGN
                     AHFSNGFETKKAGFFERQDQSIVIGKKYGAPLYSNGFEHVLVFAPTGSGKTRSIGIPN
                     LFNYPYSVVCNDVKLTLFRTTSGYRERVLGHRCFCWAPADENRITHCYNPLSLISEDK
                     IQRLTDIQRIAHILMPDNKKSDPIWQQASRKLFKVAVLYLLDTPERPTTLGEINRLVK
                     QAGFDDWLASVLEETNHLDPEFYRNGYSYLNNHEKTRSSILETFSGYFELFDDPTIDA
                     ATARSDFDLRQLRREKITIYIGFTDDDMERLSPLLTLFWQQLISVMIKNIPDPVDEPY
                     PLLCLIDEFSSLGRIERLRRSLKLLREYRVRCVLIMQYIAQTYEQYTHDEAKAFTNIK
                     TKIAFATEDIFDAEYVSKLLGTRTVKVSAGSTSTQTQGYSESKSYNYQAIPLLRPDEV
                     MRLPEDQTLIMRTGHAPVKAAQMVWYLDTTMKHLACGSTEVPRQNVTHHPFVHQASDK
                     SLMPEALEL"
     gene            13808..14605
                     /locus_tag="ACF2SP_001465"
     CDS             13808..14605
                     /locus_tag="ACF2SP_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HFD2359255.1"
                     /translation="MNHLKIIGTAGLFMMSCAAQANDKELWAHIEALYSKIGTLQTQI
                     NSIPQGAPGPMGPAGPKGERGPAGTYTAGEGISISNGEIKTTLTHHIGEEYNGGIIFW
                     VDKTGQHGLIASKTDLNNNEGIQWRNGESGNKVTNARADGIYAGESNTKLIIAQQTID
                     NQSGTFAALLAANYRVLGDGITPCPIPTPESATCYGGWYLPSAYELTLLHNNLQRQGI
                     STFAPDYYWSSTESSVGEAWLQNFSTGELMASGKASTLGHVRAVSRF"
     gene            14839..15600
                     /locus_tag="ACF2SP_001466"
     CDS             14839..15600
                     /locus_tag="ACF2SP_001466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359256.1"
                     /translation="MRFTMITPVSPIYLKQEAKKLKKIQGLSMGKALDEVSKKLGFSN
                     YRHYLNVYESNLKEPAPSKEALLKMISSERDMLKKMKIATSFIQQFKTPFRDVLNIIN
                     QFQHSRKAVQSICGKLNLMKKEIQSFLLNDFLSEEGQDEINFRAPYFIAKKIFISHLD
                     YEINANALNVNGQYVLQTEFELELDHNDPLSKDARFNDREFEGSFRVEINKDKTITLI
                     HSDMSLDSRLEPMHGFTEEEVEDYYNRFPNERGLI"
     gene            complement(15724..18363)
                     /gene="traA"
                     /locus_tag="ACF2SP_001467"
     CDS             complement(15724..18363)
                     /gene="traA"
                     /locus_tag="ACF2SP_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HFD2359257.1"
                     /translation="MAIAFAQASIHSRAKGHSAIAASSYRSGAKLYDARTGITHDYSN
                     RHDVIYSDILLPEGSPEAFSEREFLWNKAELAEKRCDAQVCKDIVLALPKELDLVQQI
                     ELARRFAQTHFVDKGIPADITIHDHHDGNPHAHILITTRRLEKTGFSKYKARDLNPAF
                     AKGFIIEKDYWGEQWRDMQNEYFIEKNLDLTVDLNHIISERHHGKLKDTDNHYLLTEK
                     TILQQARQEVLLNDIDNVINHISAQNSVFTRRDVERLVFKTFRPSNTPQNYLHWVEQI
                     MGHEDLIELGTNERGQLCYTTRHQYIQEAKLRDDIEAMIKNKDGIGGKGIDNIIKNYP
                     LSDEQLEAVRYITGGSQISVVIGRPGTGKSYLLKPIKEHCEQNNYLVIGAALSGKVAK
                     SLQTDTGIPSSTIASLTYKLANQQLKLNSNDVLIIDEAGMVDFASMALLIREAKKAGS
                     KLILVGDPDQLKPIHKGEIFRGIAALTGYIELEHIKRQNDLGDRLASMNMAKGMIVEA
                     VDHYHEKGAIVFSETTEAAAQNLIHDWQADITNTNLQDSVVLAFTRASVGYLNEKARL
                     ALKEKQILGQEEIAFQGFKKPLKIAVGERLLFRQNDKILGVRNGDLGTVQSIKKDQFQ
                     IKLDSGELLTIPNSYNKIDYGYALTVHKSQGMTVRHSKVLIDSKYWDRHLSFVAMTRH
                     KDSLKIYADSINHPTIKELKQTLSRSTTKDNVIDWPLDFATRAGFNPDQLIGRVLNHI
                     AGVGHKIKSAFNYVVHYEAYLVDRQFRGLQEDKTELRAKAKLIADRQDQSTQEITISN
                     SKETVYKELQKNHPTLAKYQKLLDKRKNLTGYFAERIDKQINAYARELAKDRQLMNHL
                     QSHLPRVAEKINRVSRHAKSHNL"
     gene            18612..18818
                     /locus_tag="ACF2SP_001468"
     CDS             18612..18818
                     /locus_tag="ACF2SP_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359258.1"
                     /translation="MSKMTVSQMEAVLLDRIEKAKIKLDKLQQKHKLEIGTLAYKHGL
                     HQFDIQRLDAAFLKLARELRNDND"
     gene            18805..19074
                     /locus_tag="ACF2SP_001469"
     CDS             18805..19074
                     /locus_tag="ACF2SP_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFD2359259.1"
                     /translation="MTTIKQIEKERQLLARCEKSLMLEKLKKRKADTRHKIELGGLII
                     KAGLHRFEKAIILGALDFSLELIKHDKHYENLFLDRGIDLFSSIR"
     gene            19168..>19354
                     /locus_tag="ACF2SP_001470"
     CDS             19168..>19354
                     /locus_tag="ACF2SP_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359260.1"
                     /translation="MLRRSLKTKNLKFLIPVLVTNMLMIGILISSNIKLDVSLLQNLY
                     LFPIKAEIASAPIIIFLC"
BASE COUNT         5732 a         4172 c         3996 g         5454 t
ORIGIN      
        1 agcaagatct gaccctgtac gtgttagatg cagttttagg ggcacaatag gtcatatttg
       61 gttatatttg ctttttttca tcttatttat tttataatta acttgataat tataaagtgc
      121 tattttactc tgttgctttt tctgacaata cctgtcttta cagattatct ttgtaacaaa
      181 ttttcttaat ttaacctcag ctcgatataa gaataaggtt atgtgattga tgattcaaaa
      241 gccaaaccgc aagtcaaatt agtacgcggt caacttcaaa ttattgcatc aaggaatata
      301 gaacatagac ggcagctgtt gtcagattgg tataagcaga aagcttttga agtttttagc
      361 aaaaggttag atcaacttct atcaacagcc ccttggataa aagaatgtcc ttcgataaat
      421 ttaagagcga tgcgttcccg gtggggtagc tgtgcggtta atgggaaaat aacacttaat
      481 actcacctta ttaaagcacc gatagcatgt attgattacg tgatactcca cgagctatgt
      541 caccattttg aacataatca cagcgataag ttttatcgtc tattaaatca ggttagcccc
      601 aactggaagc aggtaaaaca atatctcgat aaagcggcca atttatatct tatgacttaa
      661 ctttgggaga cttataacat ctaagattct gaactaaaac accgataatc ttaacgttaa
      721 tatcatcact taccttaata tttggccaat tatcatttag tgttaacagt tcaaattctg
      781 aagaagtata agataatttt ttatactgac atatgatcgt ttctggctta ccattgattt
      841 taactgctac aaaatcaccc ggttctggca aaacagaagg ctcaatcacc aagacatcgt
      901 tgattctaat ttcaggcatc atactgtcat ctgttatttt caaggcaaat gcatccgtgc
      961 ttaattctgg ttgtaaagac gtactcacag aaataaaaac cttatcgcca gatgactccg
     1021 caccagtatg caatgaagcc tcacaagcct gccgataatc caaaagcggt attaactgac
     1081 taggattttt cgcttccttg aactgcagct catccgacaa acacatcaaa taagcaggcg
     1141 aaacatccag tgcccttgcc agcgatttta tctcttcagg ccccggagta cgaacaccct
     1201 gttcccaatt tgttaggcgc gtttgtttca accccccagc aagctcgcca agagccttta
     1261 aagtcaaacc ttttgccttc cgtgcctcaa gaatacgttt gccaatttcc tttttgatat
     1321 tcagttcagt caaatttacc cctaaaaatg agagttaact attgcaatat cactcttggt
     1381 gatataaatt agttataaat tcacatattg tgaataataa tcttttattt ttataaagat
     1441 atcacattat ggaaataaaa acgaggatga atagggatat tacccatgat ggattttgtg
     1501 attaatggtg atgagttaga ggcaatgagt gggttacccc atttccagca actaacttat
     1561 ttaagaggga tacgacctta tatggacgtt aaaaccggaa tagtcggcat caaaaggcgc
     1621 atcagtcatc agtctatttc tgagcagctt tatgttgaac cgcatcaagg cataaaaagc
     1681 cagaattttt cgcgtgatca ggttcggcgt tcggtttcag gattggttcg tgctggctta
     1741 atcgaagtgc aatccgaagg gatgcatctg attttaaaat gtcttttggc ttcatcgcat
     1801 tattctgccc aaaataaagc cgccataaac ccgccacaga aagccaccat aaagccgcgc
     1861 ggggaatccc ttgaaaatac tgggctaacc gagtttgagg cactaaaagc cgacacagta
     1921 gaatcccaaa aagccgccat acctcataaa gataattatt atatttattt attatcgcaa
     1981 ttcgaaaaat tttggtcgct ttatcccgag aaaaaatcaa agaacaacgc acaagccgta
     2041 tttgaagagc taaagcctaa tgctgaactg tgccatacca tcatcaacgc actgacagcc
     2101 caaattaatt atcgtgacac catgaaacta caaggcactt gggtgccacc ttggaaatac
     2161 ccagcaaatt ggcttgctca acgctgctgg gaggatgaat tacctacgga tgcattacag
     2221 gagaagaacc atgcagagca tacaaaaaat actcgtaaac gtcacgccac agaagacctg
     2281 ttctgcccac cctgtgatgt caaagaccga gagcacaaca atgtcatcca gctccaaaga
     2341 taccttcaaa gctgagagac gtattgattt gctgttctcc aaatttgctg ccttttatgg
     2401 ccatgtttgg agaagtcagt ttaaggatga agtgtttttg aaatttgcca aaaaagaatg
     2461 gcaagaagca ttggctgatt ttactgatat ggtactgacc agggcaattt tgaattgccg
     2521 tgagttttat gaattaccgc ctacgctgcc gcaattgttg tattgctgca gacaaatcag
     2581 gaaacaagag tcattttatg tggtcaagga tgtgtatcaa cctgcaaaca aagccgttgt
     2641 gagttcctgt ttgcagaagt gcaaggaact tttagcaaaa taactggaga acatcatgtt
     2701 ggttttaaca cgaaaagcag ggcaacagat tttaataggc aagggcttga ttcaaatgaa
     2761 ggtattgaaa gtagaaaacg acatcatcag cattgggata aaggccccat cgcacatcga
     2821 tatcgacaga gaggaaattt attttcgtaa actgcgacaa gaacaagaag cagccaacga
     2881 tgcggagatg gcgatatgaa aacggacaaa caactgagca aaagagcctt tcaaaaaatc
     2941 agaaccttgt attggggaac tgtttgtttg tgcgcgcctt ccgtctcttc tgcgcaaaac
     3001 attgaaagca tcattaaccg aaccatcaat tacttgcaag gaggccttgc acgaaccgtt
     3061 ggcgtttttt gcatcatcat tgcaggttac ctgtgtttgg cacgtcaaaa atttcctaaa
     3121 gaatattttg tgatgattct ggtaggaatg gggatcattt ttggcggctc ttccctgtat
     3181 tcaacactca ttggatagga gattcttgtg agcgatttgg aaataagccc catattcaat
     3241 gcgctgaccc gtccggcaat gacggctggt gtgacctttg aataccatat gttaaacctc
     3301 attgtctcaa tgtgcgcctt tattggattc tccccactgt acgggataat ttttattcca
     3361 ctgcatgttt ttggatggct agtgtgtcga tacgacaccc acttttttac catctgcgca
     3421 aagcgtttct tattgccaca agctcccaat acgtcattat ggagggttcg cgcttatgaa
     3481 cctttctaag acattaaagc acgtttatca ggaagcaaaa gtgtctaatt tgtttcctgt
     3541 aacgcatctt aatagcccct gcatctttga gtcgcaatca ggcttggttg ggtcggtgat
     3601 taaggtgaat ggtgcggctt ttgaaatcga agaagctgag actctaaatc accaacgctt
     3661 cttgctccat caggcattag tgagtttgga ttcgcgtttc attatttatg tgaccacgca
     3721 ccgacaaaaa atatcatgcc ctttgattgg ggaatttaag ccaggatttg ccaaggatct
     3781 ggatgagcgt tatcaacaac gatttaaaga taaaaacctg tataaaaata ccctgtacat
     3841 tactgttgta cttaaagggg atgacaccag taaaacagga tcttggttgc agtgggcaaa
     3901 aagcctctcg atgaagggaa acagtgagct tgctgaacac catagagaac aaaacatcca
     3961 aatcctaaat cgtgctgttg agcaattgca tacgaacctc aactcttttg gggcatcgat
     4021 actcggcatg caggatgagg aattaggttt tagcgagctg cttcaattcc ttgggttggt
     4081 tgtcaatgca ggccaaacag cgtcattcaa acatcctgtg tacagcccac caatagcgaa
     4141 ctcgatccct cagaccttca aacaagaggc caaatacccc gaaggacacc tcggccaata
     4201 tttaagtagc tgtcagcttt tatttggaga gtgcatccag tttcagggaa atactcaaca
     4261 ggattgtcta tttggtgcga tgctgtcact caaaaaatac cccaccaaca ccgccagtat
     4321 cctcttggac tccatgttat cgctggattg tgaatttatt gccacccaca cttttgcccc
     4381 cattggccgt gacagtgcct tggatacgat ttctaaaaaa cgctccaaac ttttaaatgc
     4441 cgaggacaag gcattaagcc aaaccactgc actaaccgat ttggaagatg gaattgccag
     4501 tgaaaccctg ttgctaggtg cacatcatca caccgtgatg ttattggcac caaacaagtc
     4561 gttattggat gcagccatac tggaagccac caaacggtat ggagctactg gagttgttgt
     4621 ggtgaaagaa acactaggac aagagcccgc tttctggagc caattgccgt gcaaccagca
     4681 ttttataacg cgagcctccc tgattacctc tcaaaatttt gtggactttt gcccattaca
     4741 caacactcaa acgagctttt ccaatgagaa ctttttaggt ggcgcggtta ccttacttga
     4801 aaccccatcg aaaacaccag tctatttcaa ctaccatgca cgaggctcta aaaccaaccc
     4861 ctccaaagga cacgccgcca ttttcggagg aaataacgca ggaaaaacca cactggtaaa
     4921 ctttcttgat gcacaaatgg ggcgttttgg tggaagaagt tttttcattg accgggatga
     4981 gtcctccaaa atttatattt tggcttccgg taacagctct tacatcaaaa tagagccatc
     5041 caatccaatc gccatgaatc ccttgcaact gccggatacc tcagaaaatc gttcatttct
     5101 gaagtcatgg tttgcaacct tggtacaaga agatagcgaa cgtgctatcc caagtcctat
     5161 tgctgaaatc atcaatgact gtatcgatta cagctttgaa caactcgccc ctgaattcag
     5221 gacgcttagt cacttaagcc agtttctacc tgttgatttt ccacgttggc cacatttaaa
     5281 aagatggttg aaacgtaatg attccagaat tgatggcgaa tttcattggc tgtttgacaa
     5341 ccagcacgat gccttaaatc ttgattttga caaggtgggc tttgatgtga cctacctgat
     5401 ggaccaagtt cagagcatca ttgcaacacc agtttatttg tatttgctgc atcgtatgcg
     5461 ccaatgcctc gatggacgtt taacctcttt tattatcgcc gaagcatggc agctttttgc
     5521 gtctcctttt tgggaaaaag cgcttcggga atggttgccc acaatccgaa agaaaaacgg
     5581 gcattttatt tttgataccc aatctcctaa aaccattacc gattccccca ttaaacacat
     5641 tgtgctggat aatctggcca ccttgatagc atttcccaat ccgctcgcag acaaggaaac
     5701 ctacatggag catttaaagt taaccgaagc gcaatttgag gcgattaaag aaaacacacc
     5761 cgaatcacgc atttttttgt ataaacaaga ccacgaagcc tttttgtgca aactggattt
     5821 aagcggttta agccaataca tcagagtact ttctgccaat acccaatccg ttaagttgct
     5881 ggatgacatc atgcaagagg tcggtcatga ccctgagctt tggcttcctg tcttttttga
     5941 aaggagcaaa aaatgaaaag aacagcctgc ttcttaattg cggttctcgg tataagcggt
     6001 gccagtcatg cggatattct gggtgtagaa gatgcccagc tcatcgtttt ggcgatgaag
     6061 caactcggag agcttcaaga acaatatcgt gtacttactg atacctacca aaccacaaga
     6121 agccagctcg ataacttgaa tcaactcaaa tcgatgaact cagggcatta tggatttgga
     6181 gactttaaca atggtttgga taccttgcaa agctggcaat cacctgtaag cacttggcag
     6241 gatgcattag aaaacctctc aggaggcaat caacaacgtt atcaagcact catggatgcg
     6301 tatgaaaaaa atcatccttc actggatgaa agcagttatg cacgttttac tactcctgca
     6361 aatgtagctc gctttaaaga agataaagcg gtcaaccgtg cggtgcttgt gcaaaccact
     6421 gagagttata acgaaatcaa caagcatatg gaagccctcc ataagctctc acaacaaatt
     6481 gaaaaaacac ccaacaccaa gggggctatt gatttgaact cccgtctcat taccgaaatt
     6541 gggtttattc aattaatgag cttaagactt caggcattaa tcagccagca agcagcacag
     6601 gaaaatttat ccgcgctcca agacagggct gaaatggcaa agtttaatcg attacccaag
     6661 tgacaaggag taaccacatg aaatggcttt tgagtgtatt gatgattgca ttattaagtg
     6721 gctgttcaaa gccccctgat ctggactctc cctgccatca ctttgggcag tattgccctc
     6781 agtatcccat taacgatgaa cctattatgg aactcacaaa atgaacacac tcatcaaagg
     6841 tctaatggtt attttaatag ctgcaacaat tagcgcgtgt catcacgaaa acccgttaaa
     6901 aacacaacct gccaaggcca gtgttaggtt tttgattaac gcctcggcca atgctgaaaa
     6961 gcaattgcac tttgccattc acaaagatgc ttatggcttt gcctatctgg aatgtatgga
     7021 aggcaagcaa agtccagaca ttcaatgtaa tgcgctatac caaggcatga ttgcctttgc
     7081 caaggaaggt cattatgcgg gcttccaaat gataacgcag gctgatttaa cagataaaaa
     7141 agtgtttgaa tcactggcag atgaatatgc cgaaacagca gccaccagct ttcctcattt
     7201 tgtttcggga agttaaatca tgaatacact acagtttgac aatttcatca tacagcttgc
     7261 aggtgaagtc gataagctga ccaatcattt tgtttttgat ggctatacgg ctcttgcaac
     7321 actgcttaaa gcccctttgg gagcaatgat tgttctttac atcattttga aaggctatgg
     7381 aattgctttg ggtgttatcg aacgacctca gcatgaattg tttcgattca gtatgcgggc
     7441 gggacttatt tacatgatgg ccatgaattg ggatctgttt tcatcctaca tgcgcgatct
     7501 gtttgtatcg ggtagtgagt ccattgccac ccgattaatg caggcggttc acaaataccc
     7561 atcaggcggt tcaatcaatc aggggttaca aaacgtattg aatgaaattc tcaatctggg
     7621 ttcggactta tttgatgcgg gatcgctcag aaaactcacg ccctattttg cgggcatgat
     7681 ggtgttttta tcgggaagcg tcacgatagg tctggccttt attgaaatcg tcatcgccaa
     7741 gttgatgttg gctgtaactt tgtgcacggc acctttgttt atcctgttta cgctttttga
     7801 ccaaaccaaa tctttttttg atagatggct tggcgtgctc gctggatttg cctttgtgct
     7861 tatttttgta tccagtgtgg taggcctttg catccactta ttgcattggg tgacttatgc
     7921 ctttttaggt aatacggatg agttaaccgc tgctatttgg gttccgattt ttatcgtggc
     7981 ctgtttatgt gtaatgggca tcacccaagc cgtggccatt ggtaaaagta ttggcggttc
     8041 ggtatgcact tcggctggct ctgccatggt gggaggcttt attggaggca gtataggggc
     8101 atggggattt tcgaagtcct ccgttcaaaa accagtaaat accctgcgcc aaggcttagg
     8161 aaacgcatcg cgtctcatgg aaaagggcaa acaactgggg gaagccagcc acaatgtatt
     8221 caagcaactg cataaaaacc tgcgaagagg agcatcatga gtaaaaccaa tttgaatgaa
     8281 tactttaagc gtgcgcgaag ttgggcagac gaccagtttg gccgaatcga acaatccaga
     8341 aaccgctatc aagccgcatt tttctcagcc atgggcttga atgtggcagc aattatggtg
     8401 atagggatgc tcgcgcatta tcaaaccgta gtaccgatgc tcgttcatca ttacgacaat
     8461 ggggtaacta cggttgaacc catagataac aaagaaaccc ccataaatcg cgcacagatt
     8521 gaaagcgaca tcgcccgtta cattcaatac cgagaatcct atgatgtatc aagctaccgc
     8581 gctcaatttg aattggtgca cctgttatcc aacagcacgg tggccaagga atatttacaa
     8641 gagcaagatg ctgcaaatac tgcctcaccc attcatgctt tggggaatca catcaagagg
     8701 gaggttcgta tttacagcat aaattttctg gattccgttt tggcgaatga aaaagacctt
     8761 cataaagatc accacgcctt ggcagaagtg gtttttagtc tcatagacac cgacaaaacc
     8821 agcggaaaaa ccacctctac acattacaac gccatgattt catggcgata caccaatccc
     8881 cctgattccc ccgaaacccg ttggaaaaac tgggatggat ttgaagtcac gcgctataca
     8941 agacaaaccc gtttaatgga ggctcaagca tgaaaaaaat actgttgagt gcggcagttc
     9001 tgctcttatc attaggcacc tatgctcaaa ataacccaac gtccttatca accgacaccc
     9061 gaattaagaa ggtggtttat cgggaaaacg atgtaatacc cgtccatggc attcccttta
     9121 ccaccaccca aattcaattt gcaaagcaag aacgggttct tgatattgaa ggtggtgaca
     9181 ccaccgcatg gatggtgacc tatcacccac aactggagaa tatgattttt gtaaaaccca
     9241 cgatgtttga ttccaaaact aatatgacgg tgattaccaa tcaacgcgcc tactacttta
     9301 gcctgaccag cactaaaaaa ctggagcagg agccaggtaa aaaaacctat gccttgaaat
     9361 ttgaataccc tcaagcccaa cccattcaca caaaaaatac gactcctaat aaaaccatac
     9421 atccaaaagt ggtcaacacc gcgtatcgat ttagtggcag tccgcagctt gttcctcgtc
     9481 atgtatttga tgacggcaag ttcacctatt ttgagctaag tagccaaggc agcgttccag
     9541 cgatttttgc ggtggatgac caaagcggaa aggaatcaac cgttaataca aggcgcgaag
     9601 ggaaatacat tgttgtgcag cgtttagccc cgcaatttac tttaaggcaa ggtggcctta
     9661 cggcttcggt ctttaacgca ccggaaatca atcgtatcaa agcaaacagg aggccaaaat
     9721 gagtatggat gataaaaagc gggaagattc tctgggcaat cggactcagg ttgctaaaaa
     9781 cccaaaacac aacgccatga aagacaagtt attgctcgca ggcgttgtaa tcatttgtat
     9841 tgtcatcggt attggcggat tactgcccaa acgaaaagca aactcggtca atgaacccga
     9901 tgaaaaacaa agtttaagca tggctttaaa ccaaaatctg gagctgattg cagcccttaa
     9961 agagaaaaat gcaacagcgc aaacaggtta taaaggtagc gatccaaggc atccccccgt
    10021 actaaaaacc atccaatcgc aaacggttag caaggaaact ctagctcgaa tgaatgcacc
    10081 ctccaccttt tttaattcag gaggtaatga ggtgataagt actgcaacgg gcggcgaagt
    10141 tcaagccagc aaaaccctga caggtcgcga tggaaattct gagtttttga atcagcaaaa
    10201 tgacattacc tctgtttctg caaaacgatt accccatcca gccatgacag tacctgccgg
    10261 agaaatgatt cctgcaacac tcgaaacggc gattaattct gagcttgctg gtatggtacg
    10321 agccattaca acacgagata tctttgcttt ggaaggcagc aagttattaa tccccaaagg
    10381 atccacgcta gtcggacaat ttaataccgc aattacccaa ggccagagcc gaatctttgt
    10441 cgtatggaat cgtctgcaga tgaccaatgg cgtgattgtc accctaaata gtccaagcag
    10501 tgatccaata ggtcgttcag gacaagcagc tgattatatt gaccgccatt tctttgagcg
    10561 ttttggtact ggtgcgcttt tgtcggtttt gggtgcctat acagcaacag gcggggttaa
    10621 tggacaggat gaatacaact ccagatcaca ataccgcatg aacattgcca gcagcttcca
    10681 acaagccgca aatcaaacac tacagcaaga catgcaaaca cgctcgactt tgcaaattaa
    10741 tcagggagct gccattaacg tatttgtagc tcacgatctt gatttctacc gtgtggcagg
    10801 gagggcataa tatggaagcc attaaagcct gcttctcacc cggcgcaaca ggtctgctct
    10861 ctcccttgca ggattttttg gaggatgaac acgtctctga aattttactc aataagcccc
    10921 aagaggtttt tatcgagcaa cacgggcaat tgactcgttt tgatattccg gtattaacgt
    10981 ctcaatacct gcggcgtttg tttttactca tagccaatga aaacaaacaa actttatcgg
    11041 agtcaacgcc cgtgctttct ggaaatctgg cggatggctc aagggttcaa ctggtgatgc
    11101 cgccagcctc cctacatgaa acactatcca tccgaaaatt tactttaaaa caggtcagct
    11161 tcgaagatta caaagccaaa ggattttttt catcggcgcg tggggtagga gttaaggagt
    11221 actcggagtg cattggtaaa accgatgggc ttcttgaact ctacgatgct aaaagctggc
    11281 aggaatttat aagacaagcc attttacaga agaaaaacat catcatctcc ggtggaacct
    11341 cttcaggtaa aacgactttt ttaaacagtt gtgtagggca aataccgctt catgaacgcc
    11401 tcattacact tgaggatact tatgaaatgg atgtacccca cagcaatatt gttcgtctta
    11461 aagcattaaa gcaaattggg gatcacgcac agaagatcac catgcaggat ttggttcaag
    11521 ccaccttaag actcagaccc gaccgcatta ttatgggtga aattcgcggg cgagaactct
    11581 tcgattttgt atcagcctgc tccacgggac atagtggtgc acttgcaacc atccatgcca
    11641 ataacccaaa agttgctttc atgcgtatgg cgcaactcta caaactgaat caggttgcag
    11701 gcatggatga gactgatatt tacaaagtgt tacatgaagt gattgatgtc atcgttcagt
    11761 tacaaaaaac gactgacggc aggcgtttgg tggaggtgta ttataaacat gcgtaatcca
    11821 tgggttgttg ccattacctt gataggtggg cttgctataa acggccaaat ggccgtgctg
    11881 ttaaccggta tcgctcaatg ggcaagtccg ttggtattgg ttgatttact aacacacgga
    11941 ttttattggg aaactgctat cgctggatgt atcaccgctc ttttgatggg ttgtgcggtt
    12001 ggctttttaa agcagggatt ttatcgatac ctttcgtttc taatcagcag cattctgctg
    12061 ggtggaatgg ttgtgtttct actgggattt gagctttata gctggatttt tttaagttct
    12121 gtacaaagcc tctcaggact catggagctt gccttccttc gttatcaaga acctgagctg
    12181 tggcatcttt tcctgacggt cgaggggatt gtgtttatcc tgacgttttt ggctattgcg
    12241 ctttatctgt ttcattggct taagcccaac aataaggcac tcggtaacgc gcatttttca
    12301 aatggttttg aaactaaaaa ggctggattt ttcgagcgtc aggatcaaag catcgttatt
    12361 ggtaaaaaat acggtgcgcc actttactcc aacggctttg aacatgtttt ggtttttgcg
    12421 cccacgggaa gcggtaaaac acgaagtatc ggcataccca atctttttaa ttacccctat
    12481 tcggtggtgt gtaatgatgt aaagctcacg ctgtttagga ccacatcagg ctaccgtgag
    12541 cgagtattag gacatcgatg cttttgctgg gcacctgccg atgagaaccg aatcacccac
    12601 tgctacaatc cattgtcctt aatttccgag gacaaaattc aacgcctgac cgatattcag
    12661 cgcatagccc atattttaat gccagacaat aaaaaatccg accccatttg gcagcaagcc
    12721 tcacgtaagt tatttaaagt ggctgtgttg tacctgctgg acacacctga aagacccacc
    12781 acgcttggcg aaataaatcg cttggtcaaa caggctggat ttgatgattg gctggcttcc
    12841 gtcttggaag aaaccaatca tctcgaccca gagttttatc gcaacggata ctcttatctg
    12901 aataatcatg aaaaaacgag aagctcaatt cttgaaacct tttcgggata ttttgagtta
    12961 ttcgatgatc cgaccattga cgcagccaca gcacgttctg attttgatct gcgccaatta
    13021 agacgagaaa aaataacgat ttacataggc tttaccgatg atgacatgga acggctctca
    13081 ccattgctaa cactgttttg gcagcagctt atttctgtca tgattaaaaa catccccgac
    13141 ccagtggatg aaccctatcc tctgttatgc ctgattgatg agttttcatc attaggccgt
    13201 attgaaaggc taaggcgttc gcttaaatta ttgcgcgaat accgtgtgcg ttgtgtactc
    13261 atcatgcaat acatagccca aacctatgaa caatacaccc atgatgaagc caaagccttt
    13321 accaacatta aaactaaaat agcatttgct accgaagaca tctttgatgc ggagtatgtc
    13381 agtaaattgt taggtacacg caccgtgaaa gtatccgcag gctccaccag tacccaaacc
    13441 caaggttatt ccgagtctaa aagctataac tatcaggcca tccctttatt aagaccggat
    13501 gaagtcatgc gtttacccga agatcaaacc ctgattatgc gaacaggcca tgcgccagta
    13561 aaagcagcac aaatggtttg gtatttggat accacaatga agcatttagc ctgcggcagt
    13621 accgaagtac cgcgccaaaa tgttacgcat catccatttg tccatcaggc atcggataaa
    13681 tcgctgatgc ctgaagcact cgaactataa accacacagg aggaaacaaa gccagtaaca
    13741 aaatcacaaa ggatgctctt taaagagatt atggattcat acagacttaa ttgatgtgag
    13801 aatcaaaatg aatcatctta aaattatagg tacagcaggg cttttcatga tgtcatgtgc
    13861 agctcaggca aatgacaagg aactttgggc tcatattgag gcgttataca gtaaaattgg
    13921 tactttgcaa acgcaaatta actcgatacc ccaaggtgca cccggtccaa tgggtccagc
    13981 aggaccaaaa ggggagcgag gacctgccgg aacctataca gcaggagaag gcatttcgat
    14041 cagcaatggt gaaataaaaa cgactctaac ccaccacatt ggcgaagaat ataacggggg
    14101 cattatcttc tgggtcgata aaacaggaca gcatggatta attgcttcca aaaccgacct
    14161 caataacaat gaagggatac aatggcgaaa cggcgaaagt ggtaataaag taaccaatgc
    14221 ccgcgctgat gggatttacg caggcgaatc caataccaaa ctcatcattg cccaacaaac
    14281 catagacaac caatcaggta cctttgccgc acttctggct gcaaattatc gcgtgctagg
    14341 cgatggcata acgccttgcc caatcccgac tccagaatca gctacttgtt acgggggatg
    14401 gtatttgcct tcagcctatg aattgacttt gcttcacaat aatctgcagc gtcagggcat
    14461 tagtactttt gctccagact attattggag ttcgactgaa tccagtgtgg gcgaagcatg
    14521 gctgcaaaat ttttctaccg gggaattaat ggctagcggg aaagctagta ctttggggca
    14581 tgtcagggct gtgagtcggt tttaattgat tagcgtacct attcttcatc aagtaagaat
    14641 aagtacgttt gagccaatcg atctcttgac atattttctt taagagataa tatcaccaac
    14701 aggttagaca gaagccttgc acctgcatac taaagctttc acgagtactg tcattgaata
    14761 aaattctata aatcatatta gaccagatat tgatcacaat gttttggaag caggtgacat
    14821 aacgttgatg agtacaaaat gaggtttact atgattacac cagtttctcc tatatatctt
    14881 aaacaagaag caaaaaaact aaaaaaaatt caaggcttat caatgggtaa agctcttgat
    14941 gaagtttcaa aaaagctagg gttttccaat tacaggcatt atctaaatgt ttatgaatct
    15001 aatctcaaag agcccgcgcc ctcaaaagag gctcttttaa aaatgatatc ttcagaacga
    15061 gatatgttaa aaaaaatgaa gattgcaact tcattcattc aacaatttaa aactccattt
    15121 cgcgatgtgc tgaatatcat caatcaattt caacactcac gaaaagctgt ccagtctatt
    15181 tgtggaaaat taaatttgat gaaaaaagaa attcagtcat tcttgcttaa cgattttctt
    15241 tctgaagaag gacaagatga aataaatttc agagcaccct attttatagc caagaaaatc
    15301 tttatcagcc atcttgatta tgaaattaat gcgaatgccc taaatgttaa tggccagtat
    15361 gttctccaga ctgaatttga gcttgagcta gatcacaatg acccactcag taaagacgca
    15421 cgcttcaatg atcgtgaatt tgaggggtca tttagagttg aaatcaataa agataaaaca
    15481 ataaccctca tacattcgga catgagtttg gatagtcgat tggaacctat gcatggattt
    15541 accgaagagg aagttgagga ttactacaat cgttttccaa atgagcgtgg acttatttga
    15601 ttacatactg gtacttaatg attaagatca gatatataat tttacaattg tagatgtagc
    15661 caagaatctc tatagtgggt agcaagcaaa ctgacattat ttatctttga ttaaatttgt
    15721 cgtttagaga ttatggcttt tggcatgtct actcacacga ttgatttttt ctgcaactct
    15781 cggtaggtga gattgcaagt gattcattaa ttgcctgtct tttgctaact cacgggcata
    15841 agcattgatt tgtttgtcta tcctctcagc aaaataacct gttaaattct tacgcttatc
    15901 aagcaatttt tgatacttag caagagtagg atgatttttt tgtagctctt tgtaaacagt
    15961 ctcctttgag ttacttatag ttatttcttg agttgactga tcttgcctat ccgcaatcaa
    16021 ctttgccttt gcccttagct cagtcttatc ttcttgcaag cctcgaaact gacgatcaac
    16081 aagataagct tcgtaatgta ccacatagtt aaacgcactc ttgattttat gccccacgcc
    16141 agcgatgtga ttgagcacac gacctatgag ctgatccggg ttaaacccgg cacgggttgc
    16201 aaaatccagt ggccaatcaa taacgttatc ttttgtggta cttcgcgata aggtctgttt
    16261 caactcttta attgttggat gatttataga gtccgcataa attttaaggc tgtctttatg
    16321 acgagtcatg gccacaaaac ttaagtgccg atcccagtat ttgctgtcaa tcaaaacctt
    16381 ggaatgcctg acagtcattc cctgagactt atgaaccgtc agcgcataac cgtaatctat
    16441 tttattgtag ctatttggaa tggtaagcag ttcgccgcta tcgagtttga tttgaaattg
    16501 gtcttttttg atggattgaa cggtgcctaa atctccgttt cttacgccaa ggattttatc
    16561 attttgccgg aatagcagcc gctccccgac agctattttt agtggctttt taaacccttg
    16621 aaaggcgatc tcttcctggc cgagtatttg tttttccttt aatgccaacc gtgccttctc
    16681 attcaaatag ccaacagagg ctctggtgaa tgccaacacg acagaatctt gtaaattagt
    16741 attggtaata tcagcttgcc aatcgtggat taagttctgg gctgcggcct cagtcgtctc
    16801 agagaataca atcgctccct tttcatgata gtggtctacc gcctcaacaa tcatgccctt
    16861 agccatattc atactggcaa gcctgtcgcc caaatcattt tgacgtttaa tgtgttccag
    16921 ctcgatgtag cctgttaagg cggctatccc tctaaaaatt tcacctttat gaatgggttt
    16981 taattgatca ggatcaccca caagaatcaa cttggaacct gccttttttg cctcacgaat
    17041 cagcagtgcc atgctggcaa aatctaccat acctgcctca tcgataatca aaacatcatt
    17101 gctgttgagc ttcagttgct ggtttgccag tttataagtc aacgaggcaa tggtagagga
    17161 tggaatgcct gtatctgttt gcagggattt agcgactttt ccagacaatg cagctccgat
    17221 aacaagatag ttgttctgct cgcagtgctc tttaatcggc tttaacaaat aacttttacc
    17281 tgtacccgga cgacctatca cgactgaaat ttgagagcct ccggtaatgt aacgaaccgc
    17341 ctccaattgc tcatcactca atgggtagtt tttgatgatg ttgtcgatgc ccttaccgcc
    17401 aataccatct ttgtttttaa tcatagcttc gatatcatcc cgaagttttg cctcctgaat
    17461 atattggtga cgtgtggtat aacacaattg cccccgttca tttgttccca gctcaatgag
    17521 atcctcatgc cccatgattt gctcaaccca gtgcaaataa ttctgtgggg tgttgcttgg
    17581 ccgaaaggtt ttaaatacca aacgctctac gtcacgtcgg gtaaatacgc tgttctgcgc
    17641 cgaaatatgg ttaatgacgt tatcaatatc attcagcaaa acctcttgcc gcgcttgttg
    17701 cagaatggtt ttctcggtga gtaggtaatg attgtccgta tcttttagtt tgccatggtg
    17761 ccgttcagaa atgatgtgat tcaggtcaac tgtcaaatcg agattttttt caataaaata
    17821 ttcattttgc atatcacgcc actgctcgcc ccaataatct ttttcgataa taaatccttt
    17881 ggcaaaagcc ggattcagat cgcgtgcttt gtattttgaa aaacctgttt tttccaaacg
    17941 gcgggtggta ataagaatat gtgcatgggg attgccatca tgatgatcat gaattgtaat
    18001 atcagcagga atccccttat caacaaaatg agtttgtgca aatcgtcttg ccagctcaat
    18061 ctgttgcacc aaatccagct ctttgggaag tgccagaacg atgtccttac acacttgagc
    18121 atcgcatctt ttttcagcaa gctctgcctt attccataaa aattcacgct cggaaaatgc
    18181 ttcagggctc ccttcgggta acaggatatc agagtaaatg acatcatgac gattggaata
    18241 atcgtgtgtt atgccagtgc gtgcatcata taacttggca ccagaacgat agctgctagc
    18301 agcaatggca ctgtggcctt tggcacgact atgaatggat gcttgggcaa aagcaatcgc
    18361 catgaattca cgctctaatc gaactgatcc atcggttgct ttaataaaag aaaaaatacc
    18421 ttatccataa aggcattttt gagtcgcagt tctaatttag caaacattcg aacgaatgta
    18481 tattgcgccc tagcttttcg cttcgggatt ttgaagttcg ctgcgctcac ttttttggat
    18541 gattttctat tgcatttaga aatcctacat tgtacgcttg gtttgttaac ctcatttgcg
    18601 gagataaaat tatgtcaaag atgacagtat cgcaaatgga agcagtactc ttggatagaa
    18661 tcgaaaaagc caaaatcaag ctggataaat tacaacagaa gcataagctt gaaattggta
    18721 ctcttgccta caaacacggc cttcatcaat ttgatattca gcgattggat gcagcgtttt
    18781 taaagctggc tagggagctg cgcaatgaca acgattaaac aaattgagaa agaaagacag
    18841 cttcttgccc gttgcgaaaa atcacttatg ctggagaaat tgaagaaacg taaggctgat
    18901 actcggcata aaatcgaact tggtggatta ataattaaag ctggtctaca tcgatttgag
    18961 aaagctatta ttctaggagc attagatttt tctctagaac taattaagca cgataaacat
    19021 tacgaaaatc tctttttaga tagggggatt gatttattct caagtattcg ttaaaaaaat
    19081 ttcataacat cttgattttt taatgatcat ccccatatta ccctgaaaat tacacaagta
    19141 ccttgcttgt ttaaaggata acatggaatg ttaagacgtt ctttaaagac caaaaatctt
    19201 aaatttttaa tccctgtttt agtaacaaat atgcttatga tagggatttt aatctcgtca
    19261 aacattaaac tagatgtttc cttattgcag aatttatact tattccctat caaagcagag
    19321 attgccagtg cgccaattat tattttttta tgta
//
LOCUS       DAXLDT010000052        19268 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXLDT010000052 DAXLDT010000000
VERSION     DAXLDT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19268
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.052"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..648)
                     /locus_tag="ACF2SP_001471"
     CDS             complement(<1..648)
                     /locus_tag="ACF2SP_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359261.1"
                     /translation="MLYNKWGYLMTTLYIKNLRYNEVAVCRALFEAAEKTEEGIKTKR
                     YGMQMTADMPGLWSPLSNYDIENDLRMARIQRNGRVREIGYLTVNLVFQFDSLDVTEY
                     ETKYGISAKDVLEKAGFTCSKDAAGYSESVSTSTRSLGQESKPEKPTQTSVVNTSFFS
                     LKSVDTDKRFTKSTKPGILYEARSYGKIEDDTLKSYGFTEGQWERQKEGIENIVII"
     gene            838..1404
                     /locus_tag="ACF2SP_001472"
     CDS             838..1404
                     /locus_tag="ACF2SP_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HFD2359262.1"
                     /translation="MKAKYDPTEPGLQKLPSEIKVMILEFLDAKSKLALSQTNYGWRD
                     LILDWPEYTKEITNTLFRLDKKRHRQAIAQMMSGRVTASSMAKLFEELLCSSIPSSYV
                     FLIFFASQNSVAFIEVLTVILVFAAITSLAHDLVDYFIESDTKAEKQHAQRRAFQFFA
                     QPSQNAVQQNSEEENLSADPKARPCEPL"
     gene            1670..2425
                     /locus_tag="ACF2SP_001473"
     CDS             1670..2425
                     /locus_tag="ACF2SP_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2359263.1"
                     /translation="MYVFRRFSKHIITFLHSLELAFRFLGHLVDSTIKVIFGKLSIVW
                     PNTLEMIYYSGARLVILLSFIGILLGATVSQTVYALLKPFQLHQRVLPIVQNILTHEI
                     LPVLIGFILCIQAALHMVNTRLEHYQENPEAIILAQVLPIIVGMNIASLLLYVYLVSA
                     IFFSFYLTFHFMLGFTNNEFLSYVVSSTTLFDLIYSVVKTLILCIIVGLTSGYYYYEA
                     SIRHIYLRKAVSRILTRGSFWLIIASMYITLTF"
     gene            2493..3179
                     /locus_tag="ACF2SP_001474"
     CDS             2493..3179
                     /locus_tag="ACF2SP_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HFD2359264.1"
                     /translation="MILGGTFFYIEYKRAQKQTFVMFFKGSLKGLVTTAPVTYRGVKI
                     GEVKVIEITENKEHSKVLIPVYVQFFVERTYGFSQDPIHLLIDNGYVANITKPNLLTG
                     VAEIELIKPTPAVKYKQTYYRSYPVFPTHNSAEKYTSMEEAFEAAKKAFEDVSELVRS
                     KEIQDTLEAIQKVSENLGQLASSLNQDVPSVVAYLNQSLKQITSAAYSTQNLTDYLSR
                     YPESLLRGKR"
     gene            3176..3901
                     /locus_tag="ACF2SP_001475"
     CDS             3176..3901
                     /locus_tag="ACF2SP_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HFD2359265.1"
                     /translation="MKNNRHFLKFYFIKDLSWLSSVRTSFIYSPFESIASSPFSSYIE
                     LRKKPVKFLLILLIALTLCDCGRSKETQFYLLTPISPQKNSRNSYNHLQIGVDEVSIP
                     DYMKKPQLMIHQTPHHLTLEEFNQWAGALDKNITLVLTTNLSTLMPGVVVQTSPWNNK
                     FHPDYHLQVMISQFEIDIHGNSILRAEYLIYRKEELIYKGNAYYHIKVPLISIETLVQ
                     SMNTNLTHLTEEIARFFIKNTTR"
     gene            4002..4412
                     /locus_tag="ACF2SP_001476"
     CDS             4002..4412
                     /locus_tag="ACF2SP_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HFD2359266.1"
                     /translation="MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTAS
                     TVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKLQAVFSDEKTKTQQRIEKIKRQK
                     RRRSRRAKQKMLDEKSRQSQLKTLRKSPRSHDIE"
     gene            4679..5563
                     /locus_tag="ACF2SP_001477"
     CDS             4679..5563
                     /locus_tag="ACF2SP_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFD2359267.1"
                     /translation="MAGTSLLTLIDDIATALDDIAVMTKVATKKTAGVLGDDLALNAE
                     QIIGIHANRELPVIGAVAKGSAVNKLILIPLAILINFFAPALVGILLILGGLYLCFEG
                     FEKIVEKIFHKKNEPAKKKIDTQTIDMRQFEKEKISGAVRTDFVLSAEIIVITLGTVA
                     ATSLINQIAVLTLISLIMTIGVYGLVACIVKLDDLGFILREKKYPLKQIGILLIGAAP
                     ILMKILSVAGTIAMFLVGGGIITHQIAAIHHFTENLSAMMSIGVDFFIGIISGAITAA
                     LYFTFKKMKSKFKLMNTK"
     gene            5731..6186
                     /locus_tag="ACF2SP_001478"
     CDS             5731..6186
                     /locus_tag="ACF2SP_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFD2359268.1"
                     /translation="MPDIKHYLKPIILFSGLLAAPFAMAEATKAEETSTVKLSTILQQ
                     LSDQGYKNIHDAEIDDGVLTVEGSNEQGVKFEIHLDPATGKILKKQEHPQPKYSIIDI
                     TKKIETEGKFKIIEIEFDEGIYKIKGETDSHEKKELHIDPNTGETKTDH"
     gene            complement(6268..7041)
                     /locus_tag="ACF2SP_001479"
     CDS             complement(6268..7041)
                     /locus_tag="ACF2SP_001479"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HFD2359269.1"
                     /translation="MYKTVHFYSNNLKLEGKLEEPTGKCLGYVLFAHCFTCGKDIAAA
                     SRIASALVSNGFAVLRFDSTGLGSSEGSFSETNFSSNVEDLVAAADYLRAHYRAPVLL
                     IGHSLGGAAVLLAAKNISEVKAIATIGAPASAHHVKHHFSADLSKIESDGEAQVTLGP
                     RSFTIKKQFLQDIDRYQDTINSDAGKALLILHSPIDKVVSIKEAEKIYKAAKHPKSFI
                     SLDKADHLLSDKRDSQYAAEVIAAWASRYLTPSSEDQSG"
     gene            7466..7885
                     /locus_tag="ACF2SP_001480"
     CDS             7466..7885
                     /locus_tag="ACF2SP_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFD2359270.1"
                     /translation="MKTYRKELWFNIPERMGFINITDKVIECLKESSIQEGLILVNAM
                     HITASVFINDDESGLHQDYKKWLEQIAPHEPIRQYRHNDTGEDNADAHIKRQIMGREV
                     VVAITEGRLDFGPWEQIFYGEFDGRRDKRVLVKIIGQ"
     gene            complement(8014..8991)
                     /gene="ravD"
                     /locus_tag="ACF2SP_001481"
     CDS             complement(8014..8991)
                     /gene="ravD"
                     /locus_tag="ACF2SP_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase RavD"
                     /protein_id="HFD2359271.1"
                     /translation="MNLKKEVFLNQNCAEMMIKKAAQLILRSDLDFEYTRGIQDIQVD
                     LGPAFMFSPDEEKTLWVSGKNQETLEKDLATLNKSSVYFFRTGTQGGAGHWQVLYYEA
                     AKSGWVSYSSQSNHFQVTDSNGKLTASGKGLLVPHANWGKENGNYAFLLVNASAQNII
                     HAANFVYILRAQDENAAIEYCALNHEFHPEIKRTARVKAEPETRTNGEIHNPPKDLSC
                     QNLKTIYSKHVALLHVINRLEAGSKSYNPYWMGSQAKLNTILEALGACYQKGLDVDNE
                     LSNSNSELSKAINIRRLPSIFSFWGGKNESPVESRKIINEKRTYRRNNP"
     gene            9227..9706
                     /locus_tag="ACF2SP_001482"
     CDS             9227..9706
                     /locus_tag="ACF2SP_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359272.1"
                     /translation="MKEKTAKQLKLFIIYLLSYWLLSIISCLIAFGYDNSLRMLLASP
                     KSDLSGALLFFSSFIATALLFVFRYKTFSDQPYPYFIFGFYVGNVSLLMLFILDAFIR
                     ELIVWKFPEFLLVFISPFVELVLSYLFFGFAFLAIIPAVTSAFILYAVQRKLLLPPI"
     gene            9883..10095
                     /locus_tag="ACF2SP_001483"
     CDS             9883..10095
                     /locus_tag="ACF2SP_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HFD2359273.1"
                     /translation="MGIVKISDELHEATKLMARAMSRSINSQAEYWIRIGKLAEENPS
                     ITYTDILKILVHQASLGEIYDVSEDA"
     gene            10076..10837
                     /gene="map"
                     /locus_tag="ACF2SP_001484"
     CDS             10076..10837
                     /gene="map"
                     /locus_tag="ACF2SP_001484"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFD2359274.1"
                     /translation="MLVKTPEEIKKMRVAGHLAASVLEMIEPYVIAGASTKELEQVCR
                     QYIIEDLKAIPSTLNHYGFPACICTSINHVVCHGIPSEKKLKNGDIINIDVTVQKDGY
                     IGDTSKMFLVGNIKPFAKKLVEVTQECLYKAISIVRPGTHLGDIGNIIQTHAEQHGYS
                     VVREFGGHGIGKSMWEDPHIMHFGKPNTGLRLQAGMTFTIEPMLNLGRKEVKTLGDGW
                     TVVTKDHKLSAQWEHTILVTDNGHEILTLRADEHL"
     gene            10952..12034
                     /locus_tag="ACF2SP_001485"
     CDS             10952..12034
                     /locus_tag="ACF2SP_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2359275.1"
                     /translation="MPCEKCDSAATYHLSGTYQLLILPPRGHCYSKLLKYLTENNFDI
                     NKRDSGIIELLFTAERAQDIGHALERCYSQAELEDSKAVLLPIDIADRNVERVLNHTH
                     SLKKIVGLCLSQWLVGLIENQGLSTFCQPILHNTTLKPYGFECLLRGKQDNNILPSSD
                     LFGAAKSSDMLFLLDKKARICHIENMSKIDIADKKIFINFNPTAIYDPLFCLTTTNQS
                     LKKTSIKPAQIVFEVTESDEVKDKKHLLKIIDYYRNQGYCVALDDLGSGYSSLNLLTE
                     LQPDYVKLDQELISQIHTNEFKQIILEKICEMATKLKIKIICEGIETEDELQIIKNYA
                     IDFYQGFLFEKPMPYEKIPDYIKAST"
     gene            12064..12942
                     /locus_tag="ACF2SP_001486"
     CDS             12064..12942
                     /locus_tag="ACF2SP_001486"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HFD2359276.1"
                     /translation="MLTNIERAKLQLIDETSDMVIITKAEPLDAPNGPEVVWVNKAFE
                     NISKYKANEIIGQTPRILQGKETNQNTLKMIKEAIKAQTNINVDLLNYTKDGTPYWIK
                     FSIVYLRDGQGNLCYLGAIEKDISAIKNLNIQLSEKAKTDPLTNVYNQENFFITGNEA
                     VKNFHQNQETVGLLFFDVDDFKGINDTQGHIYGDKILKKVAQESLKLIRKTDNVFRYG
                     GDEFAIIFSGINVKILEKKAQQLQKKLAKSHISVSIGGTISKQSDKSINTLLARADEA
                     LYYVKANKKGLFFIRE"
     gene            complement(13290..14036)
                     /locus_tag="ACF2SP_001487"
     CDS             complement(13290..14036)
                     /locus_tag="ACF2SP_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359277.1"
                     /translation="MKRYIWFHPSYVSESAEATLLGQQTNAKDHGYILLNDQDLGKVT
                     CEDHLTIMGHSTLAPTPAEDTGLYMQGDTADDLIGRLTTNGLKEAPKILSLESCRAGI
                     SGGLAQQLSRSPFFKYTLIEANLNSVGRKPMEMQWNFPTDSLGRAVLKLDNSPWMFYL
                     GGFLVGRLTHDTYTTEDLFSLLSPPTFHKHFFAHYQPGFFGGRSGRHCLFNKTTITLE
                     KASNFAHEDQDSASAKALEVVLKKLYFQPA"
     gene            complement(14297..15733)
                     /locus_tag="ACF2SP_001488"
     CDS             complement(14297..15733)
                     /locus_tag="ACF2SP_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359278.1"
                     /translation="MTPLFPQKGHPIAIKQGRSGSCFLLACLDCLLNGEAEGIDVVRK
                     KFKQTTSGVTLRIKRTDNTMKVQWEKMAGKYTYSYDPRTDEDVIFIDNEKLKEIDNSK
                     KGSSSNSLAVKILEHISAYYYQIAWDGESLLAHKLHPKFRNNPIAKQSDTAFLAKLLG
                     VHVTSFYDPRKLVKLKTIDPMIPVYVGMNFGKLDRLGRHRRHALRIGNIKPLDDGEYE
                     VTLINPWNNQKQTTCLLSALRKRKCHFAVFDSSPQKKLLTEIFLEYKTEIGCELFRNK
                     ELAFLLCEMKQCGLLDSELAKYPLSSCMVLYKKLPNLVANYRRLPYNERYNMLICVAN
                     SNGHVRLFNKLMQGLSVVDKPSMSRSPQRVYPSAKSFFADLFASLEASLERVHYNDLL
                     KTLRVNYGHLRSVNTALNQVENRQGIYSKMELRNMNQRLERLGFFMKCHTDPRGYRHP
                     DLGQQPNFCNRSTEKVNAKSHQDKGVSL"
     gene            16091..16342
                     /locus_tag="ACF2SP_001489"
     CDS             16091..16342
                     /locus_tag="ACF2SP_001489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359279.1"
                     /translation="MVKGLPSALEYRNDLYYWPPNVAISPDTTNLFITMDGIDKVCFL
                     NTGLQGGLEQISEISIIINGAKKDWNCFPYTTTILEARP"
     gene            16737..16907
                     /locus_tag="ACF2SP_001490"
     CDS             16737..16907
                     /locus_tag="ACF2SP_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359280.1"
                     /translation="MVTMLLPIGHKVPGINALANGLGRFHSGLACPKLPFLFNPLRLK
                     ITGTWDGNPPRF"
     gene            complement(17232..17747)
                     /locus_tag="ACF2SP_001491"
     CDS             complement(17232..17747)
                     /locus_tag="ACF2SP_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFD2359281.1"
                     /translation="MFNVSIRKATSLDAEAIASVHVHSWKKAYQQFIPEIVLNSLSIP
                     ERTQLWNELLNNGVTVLVLEIDNQIIGFASICTFRDGIEDNSKGEISAIYIHPNFWHQ
                     GFGKKLSLAALKTLKELGYTEALLWVLSDNHQARSFYEGMGFELNDKSKLAEFYDGGA
                     LLKEVLYKKKL"
     gene            complement(18120..18371)
                     /locus_tag="ACF2SP_001492"
     CDS             complement(18120..18371)
                     /locus_tag="ACF2SP_001492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359282.1"
                     /translation="MRWFISKEASIPSQIQDGPARLNGSQEADEGIKMHGGSTILSPK
                     NKSSRVRFYSSPVLDAEKPAMRFFSSLSTEPVSSNNSPI"
     gene            complement(18498..>19268)
                     /locus_tag="ACF2SP_001493"
     CDS             complement(18498..>19268)
                     /locus_tag="ACF2SP_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359283.1"
                     /translation="YEYAYWAKDSHMQRMLEKYIRQDDETRQFMLERVKAIEELVNPP
                     EAERFFAHPKPRGLHYTTQNKEGKTIDHWEAHVDLTPLKKALQHFLDEFKKLYNNPKP
                     NNYREVLNNIWVKEVGMAQRCVPAHIAQEYCRVGWDLFLRLKDNKALFDASNPNNLKR
                     QLKIFDWNTYTYDLWFTPGSHAVDCGLGFSCALIRGGNNCPRPTNMYDRWTTPNEVVE
                     QDLEMISIIDEVRTCDLKQSLENLSQPLIAQAAQYLSI"
BASE COUNT         6045 a         3796 c         3507 g         5920 t
ORIGIN      
        1 aataatcact atattctcga tgccttcttt ctgcctttcc cattgtcctt cagtaaaacc
       61 atagcttttc aaagtatcat cttctatttt accgtaggat cttgcctcgt ataatattcc
      121 tggttttgtc gatttggtaa atcttttgtc tgtatccaca ctctttagag aaaaaaatga
      181 ggtattaacc acacttgtct gggttggttt ctcgggttta ctttcttggc ctaatgacct
      241 tgttgaagtt gaaacagatt cagaataacc cgctgcatct ttactgcatg taaagccggc
      301 tttttctaaa acatcttttg ctgaaatgcc atattttgtt tcatactctg taacatcgag
      361 gctatcaaat tgaaatacaa gattaactgt caaatatcca atttccctca ctcttccatt
      421 gcgttgaatt cttgccattc tgagatcatt ctcaatgtca taattagaaa gaggagacca
      481 taaaccaggc atatcagccg tcatttgcat gccatatctc ttggtcttta ttccttcttc
      541 agtcttttca gccgcttcaa ataaggcacg acagactgct acttcattat atcttaaatt
      601 ttttatgtaa agcgttgtca ttagataacc ccatttattg taaagcaggc attttatcca
      661 tttttgctca aaataaaagc gtgtttaccg attgaactta atgaaaacgc attaattact
      721 cccgcatatc aatattttat atagccccta tacaaataaa tcatttttga cacaatgcaa
      781 aactacctaa aataaatttt aattttttca gaaaataaat aatttaggtt attcactatg
      841 aaagcaaaat acgaccccac agagcctgga ctccaaaagt taccctctga aatcaaggta
      901 atgattcttg agtttcttga tgccaaatca aaacttgctc tttcacagac aaattatggt
      961 tggcgtgatt taattctaga ctggccagaa tataccaaag aaataacgaa tacattattt
     1021 cgtcttgata aaaaacgcca tcgtcaagca atagcacaaa tgatgtcagg aagagttaca
     1081 gcaagttcta tggctaagct atttgaagaa ttactatgtt ccagcatacc ttcgtcctat
     1141 gtgtttttaa tcttcttcgc atcgcaaaac tctgtagcgt ttatagaagt cttaactgta
     1201 atccttgtat ttgctgcaat cacttctctt gcccatgatc tggtggatta ttttattgaa
     1261 agtgacacaa aagctgagaa acagcatgca caacgccgtg cttttcaatt ctttgcccaa
     1321 cccagtcaaa atgctgtaca acaaaactcg gaggaagaga atttaagtgc tgatcccaag
     1381 gcccggccat gtgagccatt gtagttcggg ttttggcccg atatttaccg aatgcctcta
     1441 cttaattctt cgatatttat tatatttaca accttatttt gatcctccac tcacccgaat
     1501 ataactagca acgaactaaa cagagtgtta ttcttggttg tagtaaaaga gcaatacgtc
     1561 tcactgctat taagttaaga attaacttct ttttgcctga tacaagcatc ttgctaacga
     1621 agctattatt cttgtcgcgg cattttgaaa gaggactaat ggattagcca tgtatgtttt
     1681 tcgccgtttt tcgaagcata ttattacttt tctccattcg cttgagctcg cttttcgatt
     1741 tttaggccat ttggttgata gcactatcaa agttattttt ggcaaactgt ccatcgtctg
     1801 gcctaacaca ctagaaatga tttactattc tggagcgcgg cttgttattc tactgagttt
     1861 tattggtata ttattaggcg ctactgtttc acaaacggtt tatgcgctct taaaaccttt
     1921 tcagctacat caacgagtac ttcccattgt tcaaaatatt ttaacccatg aaatattacc
     1981 ggtacttatt ggcttcattt tatgtatcca agccgccttg catatggtta acacccgttt
     2041 ggagcattac caggagaatc cagaggcaat tatccttgct caagtattac caatcattgt
     2101 aggtatgaac attgcctcct tactgttata cgtttatctt gtatccgcta ttttttttag
     2161 tttttatctc acttttcatt ttatgctagg gtttacaaac aatgaatttt tatcctatgt
     2221 tgtcagctcg acaaccttat ttgatctgat atactcggtc gttaagacat tgattttatg
     2281 catcatagtt gggttgactt ctggttatta ttattatgaa gcatctatta gacatattta
     2341 tttgagaaaa gcagtttctc gaattttaac gcgtggctca ttctggctga tcattgccag
     2401 tatgtatatt actcttacgt tttagggacg acgtgcataa cgaacgattc tatacattag
     2461 tgggtatttt tatagtaggc gccctatgtt taatgattct aggcggtact tttttttata
     2521 tagaatacaa acgcgcacaa aaacaaactt ttgtcatgtt tttcaaaggc tctttaaaag
     2581 ggctagtcac tactgcccca gtaacctatc gaggtgtgaa gatcggtgaa gtcaaagtca
     2641 tagaaatcac tgaaaataaa gagcacagta aagtactgat tcctgtttac gtccagtttt
     2701 ttgtcgagag aacctatggt tttagccaag accctattca cctgcttatt gataatgggt
     2761 atgtagcaaa catcaccaaa ccaaacctgt taacgggcgt tgctgaaatt gagttgatta
     2821 agccaacccc agcggtaaaa tataaacaaa catactaccg ttcttatcct gttttcccga
     2881 ctcacaattc ggctgaaaaa tatacatcta tggaagaagc gtttgaggcc gcgaaaaagg
     2941 cttttgaaga cgtcagtgaa ttagttcgtt ccaaagaaat tcaagatact ttagaggcca
     3001 tacaaaaagt ttctgaaaat ttagggcaac tagcctctag cttaaaccaa gatgtgccca
     3061 gtgttgttgc gtatttaaat caaagcttaa aacaaattac cagtgccgcc tactccactc
     3121 aaaacttgac agattatttg tctcgatatc ctgaatcttt acttcgaggc aaaagatgaa
     3181 aaacaatagg cattttttaa aattctattt catcaaagat ctgagttggc taagtagtgt
     3241 tcgaacctct tttatatatt ccccctttga gagcatcgca tcctcgcctt tttctagcta
     3301 tattgaactt cgaaagaaac ctgtgaagtt cctgttaatc ttgctcattg ccttaacact
     3361 ctgcgattgt ggtcgaagca aagaaactca attttattta ttaacaccta tttcccctca
     3421 aaaaaacagc cgtaactcct ataatcattt acagattggt gttgatgaag tgagtattcc
     3481 tgattatatg aaaaaacctc aattaatgat tcaccagaca cctcatcacc taactctcga
     3541 agagtttaac caatgggctg gggccttgga taagaatatt actcttgtat taactactaa
     3601 tctttcaact ttaatgccag gagtagtagt tcagacctca ccctggaaca ataaattcca
     3661 cccggattat catcttcaag taatgatttc acaatttgaa attgatattc atggtaacag
     3721 tattttaaga gccgaatacc ttatttaccg aaaagaagag ttaatctaca aaggaaatgc
     3781 ttattaccat attaaagtcc ctttaatcag tattgaaacg cttgtgcaga gcatgaatac
     3841 gaaccttact catttaactg aagaaattgc acggtttttt ataaaaaaca cgactaggta
     3901 atatactcat agtgtcttca aattaaggaa aattattttg attgaattca gctatgataa
     3961 tgctttaaag gcattatttt atgggataac aaagagactt catgattggc aaagataaat
     4021 gggaaatact gaccgagaag atgaccaatc ttcatatcta tgaaacagat ctcagcgaaa
     4081 aatttatact aggcagtggt aaaggcggac aaaaactaca taaaacggca tcgacagtct
     4141 atttgaaaca tttgcctacc ggtatggaag taaaatgcca ggaatccagg agtcgtgaag
     4201 ataaccgcta ttttgcgagg caacggcttt gtgagaaatt acaagccgta ttcagcgatg
     4261 aaaaaacaaa gacacaacaa agaattgaaa agataaaacg ccagaagaga cgacgctcga
     4321 gaagagccaa acaaaaaatg ttggatgaaa aatcaaggca gagccaactc aagacattaa
     4381 gaaaaagccc acgatctcat gacattgaat aataggcaca ttaaccactt accgttttag
     4441 ttaaatcaaa ataccgggta acatccccaa agttttttgt agactcctga actttaaccc
     4501 agggacatta ttttcagttc gcttcgatca attccttctc ccacgtctgg tgattttgaa
     4561 attgaaattt ttatatcgtc aggttcgagt ctttgaaaag gagataagac atctttttgc
     4621 aatgctttga atttgcttta gtattgaaag gaatcattaa attaaggtca gctccgatat
     4681 ggcaggaaca agtttactaa cattgattga cgatattgcg acggcactgg atgacatagc
     4741 tgtcatgact aaagtggcaa ctaaaaaaac tgccggggtg ttgggcgatg acttagcact
     4801 caacgccgag caaataatag gcatacatgc gaacagagaa ttacctgtca taggagctgt
     4861 agccaaaggc tctgctgtta ataaattaat attaattcca ttagcgattt taataaattt
     4921 tttcgccccc gcactggtgg gcatcctatt gattttgggt ggattgtatt tgtgttttga
     4981 aggttttgaa aaaattgtag aaaaaatttt ccataaaaaa aatgagcctg caaaaaagaa
     5041 aatagataca cagaccatcg atatgcgcca atttgaaaaa gaaaaaatta gcggtgctgt
     5101 acgaacggat tttgtattat cagctgaaat tattgtaatc accctaggta cggtcgcggc
     5161 gacatctctg attaaccaaa tagcggtatt aactttgatt tcattaatta tgaccattgg
     5221 tgtttatggt ttggttgcgt gcattgtgaa attggatgat ctgggcttca ttttacgaga
     5281 aaaaaagtac ccgcttaaac aaattggaat tcttttgatt ggagcagccc cgatacttat
     5341 gaaaatttta agcgtagcag gaacaattgc catgttttta gtaggagggg ggattattac
     5401 tcatcaaata gctgccattc atcatttcac agaaaacctg tctgcaatga tgtctatagg
     5461 agttgatttt tttataggaa ttatttcagg tgcaattacg gctgctctat attttacatt
     5521 caagaagatg aaatcaaaat ttaaactaat gaatacgaag taagtactat aagagtcgaa
     5581 atccatagta aaatcaaagt aactttgcat tccatcgctc aaatgactat agttaaacaa
     5641 tgatttttta acaccaaacc tcatcaagcg attctttttt tgtgatttat ttgaatttta
     5701 ataccacact atcaatagga gataagcagg atgccagata tcaaacatta ccttaaaccc
     5761 atcattttat tttcaggact attggctgct ccttttgcca tggctgaagc aacaaaagct
     5821 gaggaaactt ctaccgtaaa attaagtact attcttcaac agttgtctga ccaggggtat
     5881 aaaaacattc atgatgctga aattgacgat ggcgtattga ctgtcgaagg cagcaatgag
     5941 caaggcgtta agtttgaaat tcaccttgat ccggccacag gcaagatttt aaaaaagcaa
     6001 gaacatccac aaccaaaata ttcaatcata gacattacca aaaaaattga aaccgaaggt
     6061 aaattcaaaa tcattgaaat tgagtttgat gaaggtattt acaaaataaa aggtgaaaca
     6121 gacagccacg aaaaaaagga actgcatatt gaccccaata ccggggaaac caaaacagat
     6181 cattaactcc acatgaaagg caagtctttc ctttccctta gtacacccaa ttatttccct
     6241 aaggaaaagg gagaggcttg accctactca accactttga tcttctgatg aaggagttaa
     6301 ataacggctg gcccaggcag caataacctc tgcagcatat tgggagtccc ttttgtcact
     6361 taacaaatga tcagccttat caagactgat aaagcttttt ggatgcttgg cggctttata
     6421 gattttttcg gcctctttaa ttgataccac tttatctata ggggagtgca aaatcagtaa
     6481 cgctttacca gcatcgctat taatcgtatc ctgatatcgg tcaatatctt gcagaaattg
     6541 ttttttgata gtaaacgagc gtggaccaag cgtaacttga gcttcaccat cagactctat
     6601 tttacttaaa tcagcagaaa aatggtgctt aacgtggtgg gcactggcag gcgcgccaat
     6661 agtggcgatt gcctttactt cagaaatatt cttggctgca agcagtacag cggcacctcc
     6721 aagactatga cctatcagca gaacaggagc tcgataatgg gctcttaaat agtcagcagc
     6781 agcaactaaa tcttcaacat ttgaagaaaa attcgtttcc gaaaaggaac cttcgctgct
     6841 gcccaagccc gtagaatcaa agcgtaacac cgcaaatcca tttgagacca aagcgctggc
     6901 aattctgctc gcagcagcta tatctttacc gcaggtaaag caatgagcaa ataaaacata
     6961 accaagacat ttccctgtcg gttcttccag tttcccttcc agcttcaaat tgttactgta
     7021 aaaatgaacg gttttgtaca ttgcatattc cttatttatc ttgattaggt gctcatacag
     7081 aacattcaaa tctatttata aatccttttt cttgctatga aattaattta attgattact
     7141 gatccagagt ttggatagct tcaaaatgtt cacatactaa caaatatagt catatttaac
     7201 ccaatttccc aatctatttc gactctttat tcactttgtg tcatggtgtc gcgaagtgta
     7261 atcaaagcaa cgatttatct gcactacacc ccacttaaag cgccgctcgt ttaaaatcat
     7321 gggagattga ccgccctctt tttccatcat tatgattcgc gccttaagac ccggatgttt
     7381 ccagaaattt tcatctattt gaaataaagc taagaatgct atatgtttgc cagatattga
     7441 tttattgcca aatcattgtc acatcatgaa aacgtaccga aaagaactat ggtttaatat
     7501 cccggagcgt atgggattta ttaatatcac agataaagtc attgaatgct taaaagaaag
     7561 tagtattcag gaaggtttaa tattggtcaa tgccatgcat atcactgctt ccgtattcat
     7621 caatgatgat gaaagcggct tgcatcaaga ttataaaaaa tggcttgaac aaatagcacc
     7681 ccacgaaccc atcagacaat accgccataa tgacaccggc gaggataatg ctgatgccca
     7741 tatcaaacga caaatcatgg ggcgagaagt ggtcgtcgct attacagaag gaagactgga
     7801 ttttggccca tgggagcaaa tattctatgg tgaatttgac ggtcgccgtg ataaaagagt
     7861 ccttgtaaaa attatcggcc aataaatcag gactcgaagg catcatgggt aacttaagaa
     7921 taaaaggtca ggcatttctt tattctctag taagccatgc ttctaattct cgcttagaaa
     7981 aaaagaaata gtcgacctta ctctactcgg ctttcatgga ttatttcgtc gataagtcct
     8041 cttttcatta attatctttc ttgattctac tggggattca ttttttccgc cccaaaaact
     8101 aaaaatcgat ggtaagcgac gtatattgat tgcttttgat aattcgctgt tgctattgct
     8161 taattcatta tcgacatcaa ggcctttttg ataacaagct ccaagcgcct ccaaaattgt
     8221 attgagcttc gcctgactac ccatccagta aggattgtaa ctcttacttc ctgcttccag
     8281 acggttgatg acatgcaaca aggctacatg cttactgtag atagttttaa gattctggca
     8341 agacagatct ttggggggat tatgaatctc accatttgtc ctggtttctg gttctgcttt
     8401 gactctggca gttcttttta tctctggatg aaactcatga ttaagtgcac aatattcgat
     8461 tgcagcattc tcatcttgag ctctcaaaat atatacgaaa ttcgctgcat gaatgatgtt
     8521 ttgtgcactc gcattaacaa gtaaaaatgc atagtttcca ttttcttttc cccaattggc
     8581 atggggaaca agcagtccct taccactggc tgttaacttt ccattgctgt cagtcacttg
     8641 aaagtgattg gattgagatg aataactaac ccaacctgat ttcgcagcct catagtacag
     8701 gacttgccaa tgaccagccc ctccttgagt accagtccga aaaaaataga cactgctttt
     8761 attaagagtc gccagatcct tttccaacgt ttcctgattt ttcccactta cccatagtgt
     8821 tttttcttca tctggactga acataaaagc aggacccagg tcaacttgaa tatcttggat
     8881 acctcgagtg tattcaaaat caagatcact ccttaaaatc aactgagcag ctttctttat
     8941 catcatttcc gcacaatttt ggtttaagaa tacttctttt tttaaattca ttggaaaatc
     9001 ccagtcattt ttggattatt ataatacaaa ataatcacat aaaaagctaa gtgattgctt
     9061 ggattataga atcatcccaa tatttgagtc acctgcaaga cagcagaaag gaacaaagca
     9121 aggaggggga agtcttactc ctttcctcgc cagtcaatgc cccaaaagtc gtgttaaaat
     9181 cccaaagata gaagctccgt ttaaaacagc ttgattggaa ttggttatga aagaaaagac
     9241 cgcgaaacaa ttgaaattgt ttattatcta tctacttagc tattggctat taagcattat
     9301 ttcatgcctt atcgctttcg gttatgataa ttccctacgc atgttgcttg cctcgccaaa
     9361 atcagatctc agcggtgctt tattattttt ctcttcattt attgccaccg ctttgttgtt
     9421 tgtttttcga tacaaaactt tttcagatca accttatcct tattttattt ttgggttcta
     9481 cgtaggaaac gtctcgttac tgatgttatt catattggat gcttttatcc gcgaactcat
     9541 tgtatggaaa ttccctgaat tcttacttgt ttttatatca ccattcgttg aattagtgct
     9601 ctcctatcta ttttttggct ttgcattcct ggcaatcatt ccggcagtta ccagtgcctt
     9661 tattctctac gcagttcagc gaaagttatt attgccacca atataaacct ccagtgtctt
     9721 ttgtttttca tttgtaatcg ctcccttgtt cttgccttgg aaaaaaggaa gcctggccct
     9781 aatctattgc ccgatttatt tattccccaa aaaaaatata ttttataatg aaatgtatca
     9841 ttttatatta aaacgctata ttatacattt tttggagaaa atatgggtat agtaaaaatt
     9901 tcggacgaat tgcatgaagc aaccaaactg atggcaagag ccatgagtcg gtcaatcaat
     9961 tcacaagcag aatattggat tagaataggg aaattggcag aagaaaatcc ctccattacc
    10021 tatactgaca ttcttaaaat tttagtccat caagcttcac taggtgaaat atacgatgtt
    10081 agtgaagacg cctgaagaaa taaaaaaaat gcgcgttgcg gggcatttgg ccgcatctgt
    10141 acttgaaatg atagaacctt atgtcattgc aggcgccagt accaaagagc ttgagcaagt
    10201 ttgtcgtcag tatattatcg aggatttgaa ggcgattccc tcaacattaa accactatgg
    10261 gtttcccgct tgcatttgta cttcgataaa tcacgtggta tgccatggca tcccctccga
    10321 gaaaaaactt aaaaatggcg acatcattaa tattgatgtg actgtacaaa aggacggtta
    10381 tattggggat accagcaaaa tgtttctcgt tggcaacata aagccatttg ccaaaaagtt
    10441 agtagaagtt acccaggaat gcctatacaa agctatttca atagttcgcc cagggactca
    10501 tctcggagat atcggaaaca taatccagac acacgcagaa caacatggat attcggtagt
    10561 acgagagttt ggtgggcatg gaattggtaa atccatgtgg gaagatcctc acattatgca
    10621 cttcggcaaa ccaaataccg gattacgatt acaagcagga atgactttca ctattgagcc
    10681 aatgctcaat ttgggccgta aagaggtcaa gactttaggg gatggttgga ctgttgtcac
    10741 aaaagaccat aaactatctg cacaatggga gcatactatt ttagtgaccg ataatgggca
    10801 tgaaatactg accttaagag cggatgagca tctatagaca tctctcctct tgcttagtaa
    10861 ctactgttcc cccctgttcc cccaagcctc gcgacggaaa acatatacta taatcaaact
    10921 aaaggaaacc cagtcaatca agggaaatag catgccttgc gaaaaatgcg attcagcggc
    10981 aacctaccat ttatcgggaa cctaccagtt actcatctta cccccacgag gtcattgcta
    11041 ttcaaaatta ttaaagtatt taaccgaaaa taattttgat atcaataaaa gagacagcgg
    11101 aattatcgaa ttattattta ctgctgagag ggcacaagac ataggtcatg cactggagcg
    11161 atgttattca caagcggagc ttgaggacag caaagcagtt ttgttgccaa ttgatatcgc
    11221 agacagaaac gtagagagag tactcaatca tacgcactca ttaaaaaaaa ttgtggggct
    11281 gtgtttgtct caatggctgg ttggactcat tgaaaatcag gggctctcaa ccttctgcca
    11341 gcctattttg cataatacca cgctcaaacc ctatggtttt gagtgccttc tcaggggaaa
    11401 gcaggataat aatatactgc cttcctccga tttatttgga gcagcaaaat cgagtgacat
    11461 gctttttttg ttagataaga aagcacgtat atgccacatt gagaatatga gcaaaattga
    11521 catcgctgac aaaaaaatct tcattaattt taacccaact gccatttacg atcccctttt
    11581 ttgcttgaca acaacgaacc aatccttaaa aaaaacgtcc attaaacctg cacaaattgt
    11641 atttgaagtc actgaaagtg atgaagtaaa agataaaaaa cacttgttaa aaataataga
    11701 ttattacaga aatcaaggtt actgtgtcgc ccttgacgat ttgggctccg gttacagctc
    11761 tttgaatttg cttactgagt tacaacctga ttacgttaaa cttgatcaag aattgattag
    11821 ccagattcat acaaatgaat tcaaacaaat catattggaa aaaatatgtg agatggctac
    11881 caaactaaag ataaaaataa tctgcgaagg catagaaaca gaagacgagt tacagattat
    11941 aaaaaattat gccatcgatt tttatcaagg ttttttgttt gagaaaccta tgccttacga
    12001 aaaaataccc gactatatca aagcaagcac ataaaaatgg attggctatt ggatttatga
    12061 caaatgctta caaacattga aagagcaaaa cttcaattaa ttgatgaaac cagcgatatg
    12121 gttattataa ccaaagcgga accacttgat gcgcctaatg gtccggaagt ggtttgggtg
    12181 aataaagcct ttgaaaacat ttcaaaatac aaagccaatg aaattattgg ccaaacacca
    12241 cgcattcttc aaggaaaaga aaccaatcaa aacacattaa aaatgataaa agaagcaatt
    12301 aaagctcaaa cgaatatcaa tgtggattta ttaaactata ctaaggatgg aacaccttat
    12361 tggattaagt tttcaatagt atatttgaga gacgggcagg gtaatttatg ttatttaggc
    12421 gcaatagaaa aagacatctc cgcaatcaag aatttaaata ttcaattatc tgaaaaagca
    12481 aagactgatc cgcttaccaa tgtttacaat caggaaaatt tcttcatcac agggaatgaa
    12541 gcggttaaaa actttcatca aaaccaagaa acagttggat tattattttt cgatgttgat
    12601 gattttaaag gaataaacga tacgcaaggg catatctatg gcgataagat actaaaaaaa
    12661 gttgcccagg aatctttaaa gcttatccgt aaaacggata atgtatttcg atatggcggc
    12721 gatgagtttg ctattatttt ttcaggcatt aacgtgaaaa tcctggaaaa aaaagcacaa
    12781 caactacaaa aaaagctggc aaaatctcac atctcagtga gcattggagg gactatttca
    12841 aagcaaagtg acaaatctat aaacaccctc ttggcgaggg cggatgaagc actctattac
    12901 gtcaaagcca ataaaaaagg attatttttt atcagagaat aatcacattt tgacgctgcc
    12961 aaattgcaag gaaattaaac ccagagacat tcaattcaat gatatcgacc tttaaacaat
    13021 tcttttatgc cacgaaaaaa taattatttt cagttttaat cgggtttctc tcaaaagtca
    13081 ttttgcataa tcatgtgtta catcatattg ttccttaata ttattgaggg tgagtctgac
    13141 taaaagtcta ttgatcagga cttacgaaaa accccaagat caaggcaaaa aatgttgcta
    13201 ataagggagt ttagataaac taaatggcta aattaaaaac atttttaaca aagagagtgg
    13261 ggtttttcgt aaatcatgtt gatgttgaat taagctggtt ggaaataaag ctttttcaag
    13321 accacttcaa gagccttggc gctggctgaa tcctgatctt catgggcaaa atttgatgct
    13381 ttttccaatg ttattgtggt cttgttaaac aaacaatggc gtccggatcg tcctccaaaa
    13441 aagcctggtt gataatgggc aaagaaatgc ttatgaaaag ttgggggaga caggaggcta
    13501 aacaaatcct ccgtggtata ggtatcatga gtgagtctcc cgaccaggaa tccccccaga
    13561 tagaacatcc aaggactgtt atccagtttt aatacggcgc gacctaatga gtccgttgga
    13621 aaattccact gcatttccat gggttttcgg cctacgctgt ttaggttcgc ttcaatcaag
    13681 gtgtatttaa aaaaaggaga gcgtgacagt tgctgggcaa ggccaccaga gattcctgct
    13741 cgacaagatt caaggctcaa tatcttgggg gcttccttta acccattagt agttaatcgt
    13801 ccgattagat catctgctgt atccccctgc atatacaacc cggtatcctc tgctggtgtt
    13861 ggcgcaagtg tggaatgtcc catgattgtc agatgatctt cgcatgtgac ttttcctaag
    13921 tcctgatcgt ttaataagat ataaccatga tccttggcat tggtttgctg gccaagcaaa
    13981 gttgcctcgg cagactctga aacataggat ggatgaaacc agatgtatcg cttcacagag
    14041 ggaatctctt ttattaaaca agatggactc aaacgataca aatttatttg ttttttgtaa
    14101 atgcaaaatt cgatatttgt tgtcttttca aatttaaaat aatgtgccca gctcaggaaa
    14161 ataacttgtg aagccaagct tgcaagaaag gcttgtccct gtttttatag tggtggcacc
    14221 caaaacccgg ggttggcagc cctccttcct attgtattgt tccggctctt tacaggtatt
    14281 tccaagttcc tggatatcaa agcgaaacgc ctttatcttg atggctttta gcattcactt
    14341 tttcagtcga ccggttacaa aagtttggct gttgtccaag gtcagggtga cgatagcctc
    14401 ttgggtcggt atggcacttc ataaagaaac ccaggcgctc aagacgctgg ttcatgttcc
    14461 ttaattccat tttagagtag atcccttgtc tattttcaac ttgatttaac gccgtattga
    14521 cagaacgcaa atggccgtag ttgactctta gtgtttttag taaatcattg tagtgcaccc
    14581 gctccaaaga cgcttccagc gatgcaaaca agtcagcaaa aaatgatttt gcactgggat
    14641 atacacgttg tggcgaccta gacatagatg gcttgtcaac taccgacaac ccttgcatta
    14701 gtttattgaa taaacgcaca tgaccattgg agttagcaac acatattaac atattgtatc
    14761 gttcgttata aggaagacgt ctgtagtttg cgaccaaatt ggggagcttt ttatacaaaa
    14821 ccatacatga acttagcgga tatttcgcaa gctcgctatc caataagcca cattgtttca
    14881 tttcgcataa caaaaaggct aattctttgt tgcgaaataa ttcacatcct atctcggtct
    14941 tatattccaa aaaaatttct gttaataatt ttttttgtgg ggagctgtca aatacggcaa
    15001 aatgacattt ccttttcctc agagcggaga gcagacaggt ggtttgtttc tgattattcc
    15061 agggattgat taaagtaacc tcatactctc catcgtctaa aggcttaata tttccaatac
    15121 gtagtgcatg acgcctgtgc ctgcccaatc tatccagctt cccaaagttc atccctacat
    15181 aaacaggaat catggggtct attgttttaa gcttaaccag cttgcgcggg tcatagaaag
    15241 aagtgacatg aactcccaaa agcttggcta gaaatgcagt atcgctttgc ttggcaatag
    15301 gattattgcg aaattttggg tgtagtttat gagccaaaag actttcgcca tcccaggcaa
    15361 tttggtagta gtaagcactg atgtgctcta aaatttttac tgcgagcgaa ttggaactgg
    15421 atcctttttt actgttatca atttctttta atttttcatt atcgataaaa ataacgtctt
    15481 catcagttct gggatcataa gaataagtat acttcccagc cattttttcc cactgcacct
    15541 tcatggtgtt gtcagtgcgt ttaattctaa gtgtaacgcc ggacgttgtt tgtttgaatt
    15601 ttttccgtac aacatcgatc ccttctgctt caccgttgag caaacaatcc aggcaagcca
    15661 ggagaaagca agacccactc ctgccttgtt ttatggctat aggatggcct ttttgcggaa
    15721 ataatggcgt cattggttac aaatatacga attgcttcag gtgctgtata tgataaccga
    15781 agactcaaat ttatcaaaat aatgcgcact tagggcattt gcaggccaaa agacaataag
    15841 gaaggtaaat ttataaccca cagatccgcc agggatcccc gtattgagca cttatttatc
    15901 ctcatctcat gggttcaaac caaatacacc aagccataat agtctattct atatggtata
    15961 ggagtatgtg agagatgccc ttaggcaatc tcattatcat attattggtt aaaagggata
    16021 agaatttatg aaacccaccc tatttttagt attactgtgg atctgcactg agtcttttgg
    16081 gacaactatc atggtaaaag gactgccatc tgctttagag tacagaaatg atctttatta
    16141 ttggcctcca aatgttgcta tcagccctga cactaccaat ttattcatta caatggatgg
    16201 tatcgacaag gtgtgtttct tgaatacagg gctgcaaggt gggttagagc aaatttccga
    16261 gattagtatc atcatcaatg gagcaaaaaa ggattggaac tgtttcccat ataccacgac
    16321 tattcttgag gcacgcccat agaaacacca aaccaccaat cgaataggcg cctcccccgt
    16381 atctactctt gtttggataa gctcacattg tccctttata accgtaaatt gtccttttaa
    16441 accatggctc aattgatttt ttaaagtaca attgttatat atttttatca ttgaggattt
    16501 ttagctggct ttttgatttt aatttgcacc caaataactt ttaacctgag gatagataat
    16561 gaaacctata aaaaacagta aagaacaaga acatattgct cccgaagctc aagaccccag
    16621 attgagctac actgaggacg atcgtcgccc actaacgagc acaacatcag caaaacatcc
    16681 tagtagaatg ttcggcaaat cggaagagcg ctcagcaagg gagagttacg atcaatatgg
    16741 taacaatgtt actacctatt ggacacaagg tccctggaat taatgcccta gctaatggct
    16801 tgggtcgttt ccactcaggc cttgcatgcc ccaaattacc cttcctgttt aatccattaa
    16861 gattaaaaat aactggtacc tgggatggta atcccccccg attttaatta gcatcaaaag
    16921 ttgagattta aatagatata taattaaata agttgcaaag gtatattgaa tgtcaatttt
    16981 taaaaaattc aaaaaatttt acagatcctc atcagagaac aggacacaaa ttcacctgtt
    17041 tcttggcttt gttataattc caataatagg catgactgtt ttatatattt gcgtaagaat
    17101 attctggatg tagcaaaaaa actttgattt ttgaccagag ttttacaacc aattaagatc
    17161 caaacagaaa agctatattc ttttccttac acagtgcaca aacccatcat tttaagaata
    17221 atgtgttgat gttataattt ctttttatac agcacctcct ttaataaggc tccgccgtcg
    17281 taaaactccg caagttttga tttatcattt aattcaaagc ccatgccttc ataaaaacta
    17341 cgcgcttgat gattatctga taaaacccaa agcaaagctt cggtatagcc tagttctttc
    17401 agggttttta acgcagcgag agacaatttc ttaccaaatc cttgatgcca aaaattgggg
    17461 tgtatataaa tagcactaat ttccccttta gaattatcct caatgccatc gcgaaatgta
    17521 caaatactcg caaacccaat tatttgattg tcaatttcca atacaagtac tgttacgcca
    17581 ttatttagta gttcattcca tagttgggtg cgctcaggaa ttgataaact attgagtact
    17641 atttctggaa taaattgttg gtaggctttt ttccaagaat gaacatgtac tgatgcaatc
    17701 gcctctgcat ccaaagaagt agcttttcta atagaaacat taaacatgaa ctgatgcccc
    17761 tttatttagc tttgggtctc gaataattta atttgtttta ccatttttat gataaaaggg
    17821 acagtatacc aaccaattaa tccctctgta acataattca atcttaaggc aatatagaaa
    17881 aaaccaccta ttaagagtca gggtgttatg tgcttgataa aatccccctg tatttgtgat
    17941 agtggacgaa attaaaaaag ctgattcaac cctgcaagaa tacttgatta aatcactacg
    18001 agaaatcatg atttctccat catttaaagg ttctattccc gggcattttg ctcaattatg
    18061 gagcattgcc aatgaacttt ttcagaaata ttgtacatga gcatttcata tggaattagc
    18121 tatatgggtg aattattgga tgagacgggc tcagttgaca aagaactgaa aaacctcatt
    18181 gcgggttttt ctgcatctag tacaggacta gaataaaatc taacacgtga cgatttattt
    18241 ttgggtgaca aaatcgttga cccgccatgc atcttaatgc cttcatccgc ttcttgagat
    18301 ccattaagac gagccggacc gtcttgtatt tggcttggaa tagatgcttc cttgcttatg
    18361 aaccacctca ttgggaaata attataaagc gctgattcca agcttgccgt cttttatgga
    18421 taaaggcaaa gagcaagatc tgtaggtgct catttatgac agccacatag caggaatcac
    18481 catggggctg ttactaacta aatgctgaga tactgagctg cctgggcgat tagcggctgg
    18541 cttagatttt ctagtgactg cttgaggtca caagtcctca cctcatcaat aatcgatatc
    18601 atttccaaat cctgctcgac aacttcatta ggtgtagtcc atcggtcgta catgttcgtg
    18661 gggcgggggc aattattccc tcccctgatt aatgcacatg agaatcccaa tccgcaatct
    18721 accgcatgag aaccaggagt aaaccacaaa tcataggtgt aagtattcca gtcaaaaatc
    18781 ttaagctgcc gttttagatt attcggatta gaggcatcaa acaaagcctt attgtccttt
    18841 aaccttaaaa acaaatccca ccctacgcga cagtattctt gggcaatatg agcaggaacg
    18901 catcgttgag ccataccaac ttcttttacc catatgttat taagcacctc gcgataatta
    18961 ttaggcttag gattattata aagcttttta aactcatcca aaaaatgctg taatgctttt
    19021 tttaatggtg tgaggtcaac atgagcttcc caatggtcta ttgtctttcc ttccttattt
    19081 tgggtggtat aatgtaagcc ccgcggtttt gggtgcgcga aaaatctttc agcctctgga
    19141 ggatttacca gttcttctat agcctttacc cgctcaagca taaattgtct ggtttcatca
    19201 tcttgtctga tatatttctc taacattctc tgcatatgag aatctttagc ccaataggcg
    19261 tattcata
//
LOCUS       DAXLDT010000053        19206 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXLDT010000053 DAXLDT010000000
VERSION     DAXLDT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19206
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.053"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..551)
                     /locus_tag="ACF2SP_001494"
     CDS             complement(<1..551)
                     /locus_tag="ACF2SP_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HFD2359284.1"
                     /translation="MEYLTPKGLIPFITELKALNILQQDDNTNIALVSEILARWVVDK
                     EWLDEKFYYVNPEVGFNSWLIHEEKDHTLAVNIVAWQSGREITPHDHQTWGVVGSIIG
                     VEKNYFWKRMDDGSKPGYAEIIREKTAVIRPAGGVIGFLPNDIHSVINESDDVAISLH
                     VYGKNLNYTGRHQYDPVNNLMQP"
     gene            complement(772..2067)
                     /locus_tag="ACF2SP_001495"
     CDS             complement(772..2067)
                     /locus_tag="ACF2SP_001495"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFD2359285.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTL
                     KINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQALLSEIKELEGNGVNVRERLRISL
                     ACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
                     KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGEN
                     ILFEGAQGVYLDIDHGTYPYVTSSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGG
                     GPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLD
                     VLDGLEVLRIAVAYKDRDGNILSRPPLAADDFDDLLPVYEELPGWQESTADVTVMSDL
                     PANARAYLKRIEEILGIPIDLLSTGPERDSTITLRGPFL"
     gene            complement(2202..3116)
                     /gene="hflC"
                     /locus_tag="ACF2SP_001496"
     CDS             complement(2202..3116)
                     /gene="hflC"
                     /locus_tag="ACF2SP_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFD2359286.1"
                     /translation="MNAFKTTLGVLIFLVLVLLFTTMFTVTQGQQGIILRLGRLVKDP
                     QTDAVKVLNPGLHFKTPFIESVRIFDTRIQTMDIKSTRIVTKEKKDVMVDYYVKWRIS
                     DLAQYFKSTGGNEFKAETLLEQQLNTLLRAQFGKRTISDAVSGGRDDVMEILRNAAEK
                     QAGELGIKVVDVRIKGIELPSNTSNAIYQRMRADMQKIANRHRADGQAAAEQIQAKAD
                     ADVTVLLAKTNSNAQRIRAVGEAEAAAIYSKAYTQNPDFFALYKSLLAYEASFHSKKD
                     ILILDQSSSFFDYFKQAMPKNDGTAAKK"
     gene            complement(3119..4261)
                     /gene="hflK"
                     /locus_tag="ACF2SP_001497"
     CDS             complement(3119..4261)
                     /gene="hflK"
                     /locus_tag="ACF2SP_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFD2359287.1"
                     /translation="MGWNEPEKGKDPWSGKNQPPDLDEALKRIQDKLKKTFFGGTGKS
                     NNNSSGGSSGGLLAVTVLLIAFILWALSGIFIVDPAEQAVILRFGKYAETVGPGPHWI
                     PRFISSKIVMNVDRVLDYSYSAQMLTSDENLVSVSLAVQYRINDLSEYLFNVANPEES
                     LQQATSSALRQVVGTTTLDQIITEGREVWGGRVQETLTKTLESYKTGILIVNVSPQPA
                     RAPESVQDAFDDAIKAQEDEKRFKEQAYAYAAKVVPIAEGKASRIQQEAEAYSKQVVL
                     RAQGEVAEFLALLPQYNAAPQVTAKRMYLEAMQKVMNKSSTIIVDSKAGNLLYLPLDK
                     LLGNQYVPQSENLKAIKNGTNVSNEEDNLILSGRDLTRPTYSQGRD"
     gene            complement(4470..5957)
                     /gene="ankC"
                     /locus_tag="ACF2SP_001498"
     CDS             complement(4470..5957)
                     /gene="ankC"
                     /locus_tag="ACF2SP_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkC/LegA12"
                     /protein_id="HFD2359288.1"
                     /translation="MDFVSEMNKILQMELEQFKEFIQKKQEEDKNYLEKLFWLPNGSQ
                     MTVLNYLIEQYSEPKLGIDDANRDLLAKIDFVLHEAKDVNVGEPLHQAIVTGKISLAL
                     HLLGVDENNFTPEESEKTDVLSILSKVRKKIEESFNHVKRCFFDVDKRDGYGRTLLSL
                     ALDTKRQELLIAILARNPIVHATTLRSSAYVPFQPIHQAVVLDYAEGITLLAGMGAQL
                     TNPLGSMRDTPVILAARLGKINALAALLELPTQSLSLESENNHLFEDKQTGHTAVEEL
                     CERMANENDKADALRGIAMLICRGAEPPRNEKMRNLLSSNRVALLKAVSTYLADKPQL
                     VDAFVERCHLRESALHNIVYADHSWGSSIRHLLGVPSEAALIVEELVTRKYSDPSFAS
                     ENVSSLSTTATENLRSERNPLKLYAEFVRRYTQAYDSQMITNRWSTMRWMIAEGNCDW
                     DTVVRYSKNHPTSRTRIVLNEMFNPIPRVHEDIESQQNSPRVVLK"
     gene            complement(6035..7159)
                     /locus_tag="ACF2SP_001499"
     CDS             complement(6035..7159)
                     /locus_tag="ACF2SP_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFD2359289.1"
                     /translation="MIKHATLISGLIVSFLASAAEMSFLAQLTEVPGASGYEKNVRHL
                     VQKQWQPYMAELKIDGMGNLIGQRKDNQQGPKLLFMAHLDETGFMVESITPDGFLKVI
                     PLGGIANSVIYAQRWTVSTPKGPVLAYSGMDSPHLLGDKKMLGSPDINALFLDIGAEN
                     KEQAQKEFAIQPGLEVTPVSEFSQLSKNRFLGKALDDRLGLAVISDALRAVKQQPNQL
                     YLAATVQEEVGMRGASTVYKATHPDIAINVEVGIADDYPMLLADRKGRIGLGKGPSLF
                     VYDKSMIPNQELINWIMELADKNNIPLQLEVEPGYGEDGSKVQASGMGVPVVNIGIPI
                     RYAHQHAGIFDKRDYDNTVKLLRLIAENLNQEVVDKIKKS"
     gene            complement(7134..7847)
                     /gene="trmB"
                     /locus_tag="ACF2SP_001500"
     CDS             complement(7134..7847)
                     /gene="trmB"
                     /locus_tag="ACF2SP_001500"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFD2359290.1"
                     /translation="MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPTPWNFAK
                     EFGRHDADTIVEIGFGMGTSLFAMAMNNPQCNYLGIEVHKAGVGSLVADLHEHQISNV
                     RVVVHDAVEVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
                     FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFEQRGERLGHGVW
                     DLVFIKNKEVTNDKACNSY"
     gene            8116..8352
                     /gene="rpmB"
                     /locus_tag="ACF2SP_001501"
     CDS             8116..8352
                     /gene="rpmB"
                     /locus_tag="ACF2SP_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFD2359291.1"
                     /translation="MSRVCQVTGKRPMVGHKVSHANNKTKRRFLPNIQNHKFWVEEEN
                     RFVTLRLSTKGMRIIDKLGIKAVLDKIRAKGEKI"
     gene            8367..8531
                     /gene="rpmG"
                     /locus_tag="ACF2SP_001502"
     CDS             8367..8531
                     /gene="rpmG"
                     /locus_tag="ACF2SP_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFD2359292.1"
                     /translation="MAAVTIKVKMESTAGTGYYKTTTKNPRNHPEKMELMMYDPKVRK
                     HVLFKEKKVK"
     gene            8739..10133
                     /locus_tag="ACF2SP_001503"
     CDS             8739..10133
                     /locus_tag="ACF2SP_001503"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFD2359293.1"
                     /translation="MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESN
                     PLLEATPVEEKEDAQINTNQNQDEYSDFQWSQLYNNLNKRNNFEENDYNFDNLHCTTT
                     NLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVSEIHASLNSDDFPLDIEE
                     IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNY
                     RQLMKNHQISEKTIHEILQVIHKLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLN
                     QDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQSRQETLLKVATCIM
                     EYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSH
                     VNTDSGGECSSTAIRAVIKKLISTENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREA
                     MGIPPSNERKVISS"
     gene            10160..10459
                     /gene="hpf"
                     /locus_tag="ACF2SP_001504"
     CDS             10160..10459
                     /gene="hpf"
                     /locus_tag="ACF2SP_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFD2359294.1"
                     /translation="MQINITGHGMDVTPALRAFTEEKFDKLERHFDHIRAINVVFDVE
                     KVRQIAEATVLVTKGELHASSESEDMYAAIDTLVDKLNRQLIKHKEKLLAHRDRD"
     gene            10650..10919
                     /locus_tag="ACF2SP_001505"
     CDS             10650..10919
                     /locus_tag="ACF2SP_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFD2359295.1"
                     /translation="MIKTKIKIINRLGLHARASAKFVSTAAKFQSHIDVTKDTQTVNG
                     KSIMGVMMLAANKGSELTLEIDGPDEERMNEALVELINNRFGESE"
     gene            complement(11148..11885)
                     /gene="pssA"
                     /locus_tag="ACF2SP_001506"
     CDS             complement(11148..11885)
                     /gene="pssA"
                     /locus_tag="ACF2SP_001506"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFD2359296.1"
                     /translation="MNKENHSGIYLLPNLLTTVSLFAAFYSIVASTKGQYEAAIVAMF
                     IGMIADSLDGRIARLTNTQTEFGAQYDSLSDMVTFGVAPSLLLYNLTLHQLGKFGWLV
                     AFIYTAAVALRLARFNTQIGKSDKRYFQGLPCPPAAAVMASFAWICFQNEWANLIVSL
                     FTAVLALIVSVLMVSNLRYYSFKEVDYKGKVPFLYVLVMILLFVAIAANPAIVLFASS
                     GIYALSGPMLTVISLHKMRKQRRNMSK"
     gene            12115..12399
                     /locus_tag="ACF2SP_001507"
     CDS             12115..12399
                     /locus_tag="ACF2SP_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HFD2359297.1"
                     /translation="MSNRKNKTIVIEGVTSQGKTFRPSDWAERMSGSLAVFKNSRIYY
                     SPLLQPSVNSEGYKCVLLDPKLKESSPQVYQAIMDFAKANNLKICGEEDL"
     gene            complement(12543..13085)
                     /gene="dotV"
                     /locus_tag="ACF2SP_001508"
     CDS             complement(12543..13085)
                     /gene="dotV"
                     /locus_tag="ACF2SP_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein DotV"
                     /protein_id="HFD2359298.1"
                     /translation="MNTIDLITILGNISQSLYPVQKLITGGAYILGVLFFLTAIGKLK
                     KIGDHRAQSSSQEKMFTPMMYLLVGALLIYLPSGMDLMANTAFGVGNVLTYSNYNPAN
                     IYSSMGFLIRTAGVLWFIRGCVLVAHSSQPGTQQGLKGLVFLIAGVFAMNFDNTIAMI
                     NSALDYFVKATLSVKAAAGF"
     gene            13169..13903
                     /locus_tag="ACF2SP_001509"
     CDS             13169..13903
                     /locus_tag="ACF2SP_001509"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFD2359299.1"
                     /translation="MQINTFPITLEESFMISPKVKHFIFNCELSPPFEYLPGQFITIH
                     FEHDGKNLKRSYSIANEPKKDNRIEFAAGYFEGGPGTELLYNLKPGDVIHINGPFGRL
                     IFKDETPGRYILVATSTGTTPYRAMLNELGQRIEKHPDLQVVILQGVQRSEEILYPDD
                     FQAFAKKYPQASFRPYLSRVQKQDLKDNEYSGYVQHAFPELNLNPTRDMVYLCGNPGM
                     IDEAFNYLKEHGFSMQHIVREKYISR"
     gene            14053..15063
                     /locus_tag="ACF2SP_001510"
     CDS             14053..15063
                     /locus_tag="ACF2SP_001510"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFD2359300.1"
                     /translation="MSYEELSNTMEQMLHEGKGILAADESNSTIGKRFASINTENTEE
                     NRRDYRLLLATTPDLEQYINGVILFEETFTQTDEHGTPIPELFANKGIIPGIKVDKGL
                     VDLANTEHEKVTQGLDGLTERLLHFKKLGAKFAKWRNVYSISEYTPSLTAIKTGAENL
                     GRYAAACQSVGIVPIVEPEVLMDGNHSIEHCAEVCEMVLHELFHTLFIHQVELEHIIL
                     KPSMVTCGKEVTPFSDPEEVADYTISVFRNNVPASVPTINFLSGGQTPQQATANLNAI
                     NQIGHQPWLLSFSFGRALQEDCLKEWCGNKANITKAQEALIKRARLNSMACLGEYQND
                     ME"
     gene            complement(15242..15997)
                     /locus_tag="ACF2SP_001511"
     CDS             complement(15242..15997)
                     /locus_tag="ACF2SP_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFD2359301.1"
                     /translation="MQPEVRSVSLITYNIHKGFGVGAVRFLLPEMRDAIAGLNPDFVF
                     LQEVQGKHRKREKRIVSWPDSPQCEYIAENIWPHYVYAKNAVYQSGHHGNAILSKYPF
                     ESFESINLSNMNRASRGILHTQLKLDNNIIHLLCVHLGLFKAERTEQCKALIQRIKDV
                     VPKNEPMIMAGDFNDWRHVISKILAENLGIEEAFVALEGQHARSFPAIKPALCVDRVY
                     FRGMKVQEVACLQGKPWRMLSDHLPLYARFELL"
     gene            16465..17313
                     /locus_tag="ACF2SP_001512"
     CDS             16465..17313
                     /locus_tag="ACF2SP_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2359302.1"
                     /translation="MKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDDIAE
                     HLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGA
                     CLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKF
                     EHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCL
                     TEIKAQTYLIWASKGFSFDSDKMKARIQAVKNIKIEKLDGGHHIHMEKPEVISRLLAE
                     FYQSIK"
     gene            complement(17315..18946)
                     /gene="proA"
                     /locus_tag="ACF2SP_001513"
     CDS             complement(17315..18946)
                     /gene="proA"
                     /locus_tag="ACF2SP_001513"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ProA"
                     /protein_id="HFD2359303.1"
                     /translation="MHPNYYLSPLAVAIALGIASPVKAADPIPLQKSSFSEVTQKFQL
                     TLPGVMKGAVVSTNSLQFIRQHTDGNKVTHVRMQQQYAGFPVFGGYAILHSKNATPSL
                     ATAKSDVKMNGVIYDGLQAELGQPKPSFVKNASMALQQFKDKYANKQVSEDQVTPMIY
                     IDEKHQAHWAYKVSVLVIHDDRIPERPTAIIDAETNKPFVQWDDVKTEKVQAKGMGFG
                     GNRKIGEYQFGKDLPLLEITRDSSVEMCFMENTDVKVVDMGHKYYSNNKPMQFTCKET
                     PDTQSTKTYYTGYSADGYDRDNGAASPTNDALYAGYVIKHMYHDWYGVEALTKSDGSP
                     MQLVMRVHYGQGYENAYWDGKQMTFGDGDTMMYPLVSLGVGGHEVSHGFTEQHSGLEY
                     FGQSGGMNESFSDMAAQAAEYYSVGKNSWQIGPEIMKEDSGYDALRYMDKPSRDGMSI
                     DVADDYYGGLDVHYSSGVYNHLFYILANQPNWNLRMAFDVMVKANMDYWTPYSTFDEG
                     GCGMLSAAKDLGYNLDDIKKSLSEVTINYQSCYVD"
BASE COUNT         6145 a         3993 c         3336 g         5732 t
ORIGIN      
        1 aatggttgca ttaaattgtt aaccgggtcg tactgatggc gacctgtata attcaaattt
       61 tttccataaa catgcagtga aattgctaca tcatctgatt cattaataac actgtgaatg
      121 tcattaggca aaaaaccaat taccccacca gcgggacgta taacagccgt tttttctctt
      181 atgatctcgg cataccctgg ctttgaacca tcatccattc ttttccaaaa ataatttttt
      241 tcaacaccta ttatgctacc cacaacaccc caagtctggt gatcatgcgg agtaatttct
      301 cgtcctgact gccaggcaac gatattaact gctaaagtat gatctttttc ttcatgaatt
      361 aaccaggagt taaagccaac ctctggattg acatagtaaa atttttcatc cagccattct
      421 ttatctacaa cccagcgtgc taaaatttct gatacgagag caatattagt attatcatct
      481 tgttgcagaa tattaagcgc tttgagttcg gttataaaag gaatcaatcc tttgggcgtt
      541 agatattcca tcattcacca ttttattgag taaacattca gataagagat aatttatctt
      601 ttatgtacat taactttagc atgaaatgaa atttagcaat gcaagaattg cttgcggccc
      661 ccaaggaaat agcttgctaa aacacagaac aataatactt aaaaggcgga aagtgtcata
      721 tcaagaaatc cttgcatgac actcatagtg gttacaaaac ttaactttgt atcaaagaaa
      781 aggacctcgt aaagtaattg ttgaatccct ttcaggtcct gttgatagca aatcaatagg
      841 aatccctaat atctcttcaa ttcgctttag ataagccctt gcattggcgg gtaagtcact
      901 catcacagtc acatcagcag tagattcctg ccaaccagga agctcctcat agactggtaa
      961 caaatcgtcg aaatcatcag ctgccagagg aggtcgagat aatatattgc catccctgtc
     1021 tttataagcc acagcaatcc gtaatacctc taaaccatcg agaacatcca atttggtaac
     1081 acagagtcct gaaatactat ttaactcaat cgagcgtttt aataaaacag cgtcaaacca
     1141 cccacaacgt ctgggtctac cagttactgc gccgaattcc tgtcctcgtt ccgcaatgcg
     1201 ttttccaacg tcatcaagta attcagtggg gaatgggcca ccgccaactc gagttgtgta
     1261 agcttttgta atgcctaaca cgtaatcaat atagcgagga ccaaatcccg caccattaat
     1321 tacagatccc acacaggtat ttgaagaagt cacgtagggg tatgttccat gatcaatatc
     1381 taaataaaca ccttgcgccc cttcgaataa aatattttca ccttgttttc tatgttcatg
     1441 caaacaggca gaaacatcgc aaaccattgg tcttaattct tcagcccatt gtaatgactc
     1501 atccaatagg gattccaaat caacagctgg ttgtttgaaa tattgtgtta atacaaaatt
     1561 atgataatcc aataattctg ttaatttatt ggcaaatcgg tctctatgaa ataaatcacc
     1621 tactcttaaa gcacgccgag ccactttatc ctcataagca ggacctatgc cgcggcctgt
     1681 tgttccaatt gcagacttgc ccatatgtgt ctcacgagcc ttatccaaag caatgtggta
     1741 agggagaatc agggggcaag ccaggctaat gcgtaatcgc tctctcacat taacgccatt
     1801 accctcaagc tccttaattt cactaagcaa tgcctgaggt gataaaacca caccatttgc
     1861 aatatagcag gttacgttag ggcgaagcat cccggatgga attaaacgaa gtactgtttt
     1921 tactccattg atttttaatg tatggcctgc attatggccc ccttgataac ggacaaccac
     1981 ttgagcatct tgtgtcagca aatcaacaat tttgcctttg ccttcatctc cccattgtgt
     2041 acccaaaaca acaacattct tacccatgat ttactctttg tttgcatacc tgtaaaaatt
     2101 aaaaatgcag ttgcatttta cgagaaaccc aactgcattt atattagatg gatgatgcta
     2161 ataaatatcg aacctgatta cagcaaaatt tcacattata attacttttt agccgctgta
     2221 ccatcatttt ttggcatagc ttgtttgaaa taatcaaaaa atgaactgct ttgatccaat
     2281 attaaaatat cttttttact atgaaaactg gcttcatagg ctagcaagct cttgtataat
     2341 gcaaaaaaat caggattttg agtgtatgct ttggaataga tagccgctgc ttccgcttca
     2401 ccaactgctc taattcgctg agcattacta ttagttttag ccaataaaac cgtgacatca
     2461 gcatcagctt ttgcctggat ctgttctgcg gcagcttgtc catcagctct atgtctgttt
     2521 gcaatttttt gcatgtcagc tctcatgcgt tgatagatgg cattactggt atttgaaggc
     2581 aattcaatac ctttaatacg aacatcaacc actttaatac ctaattcacc agcttgcttc
     2641 tcagcagcat tacgcagtat ttccataaca tcatcacgcc ctcctgaaac agcatcggat
     2701 atggtacgct taccaaattg ggctctgagt aaggtattta actgttgttc tagcaaagtt
     2761 tcagctttaa attcatttcc acctgttgat ttaaaatact gggccaaatc tgaaattcgc
     2821 catttcacat aatagtcaac catgacatct tttttctctt tggtcacaat acgggtcgat
     2881 ttgatatcca tggtttggat gcgtgtatcg aaaattcgca cactttcgat aaaaggggtt
     2941 ttaaagtgga gcccaggatt taacactttc actgcgtcag tttgaggatc ttttaccaaa
     3001 cgtcctaaac gtaaaataat accctgctgt ccttgcgtga cagtaaacat agtggtaaac
     3061 aaaagcacta gtaccaaaaa gattaagaca cccaatgtcg ttttaaatgc attcattctc
     3121 aatctctccc ttgactataa gttggtcttg tgagatctct tccagaaaga atcaagttgt
     3181 cctcctcatt actgacattc gtaccgtttt ttatggcttt tagattttca gattgcggaa
     3241 cgtattgatt accaagtagt ttatccaaag gtagatacaa taaatttcct gctttactgt
     3301 caacaataat cgtgctgctc ttattcatta ctttttgcat agcttcaaga tacatccgtt
     3361 tagccgtaac ttgaggagct gcattgtatt gaggaagtaa ggctaaaaat tccgctactt
     3421 ctccctgtgc ccttaagacg acttgcttcg aataagcttc tgcttcctgt tgaatacgac
     3481 tcgctttacc ttctgcaatg ggcaccactt ttgctgcata agcataagcc tgctccttaa
     3541 agcgtttttc atcttcttga gctttaatcg cgtcatcaaa agcatcctga acactttctg
     3601 gagctctggc aggttggggt gacacattaa cgattaaaat tccggtctta tagctttcta
     3661 aagttttcgt cagagtttct tgtactctac ctccccaaac ttctcgacct tcagttatta
     3721 tctgatccag ggttgtcgtg cctacaacct gccttaaagc actggaagtt gcctgttgta
     3781 agctttcctc aggatttgcg acattaaaca aatattcgct caaatcatta atgcggtact
     3841 ggacagccaa agagaccgat actaaatttt catcactggt aagcatttga gcggagtagg
     3901 agtaatctag caccctatcc acattcataa caatctttga tgagataaaa cgaggaatcc
     3961 aatgaggacc aggaccaact gtttctgcat atttaccaaa gcggagtatc acagcttgct
     4021 ctgcaggatc cacaataaaa ataccagaaa gagcccaaag aataaaagct attaataaaa
     4081 ctgtgacagc aagcaatccg ccactggagc cacctgacga attattattc gatttgcccg
     4141 tgcctccaaa aaatgttttc ttcaatttat cctggatacg tttaagagct tcatccaaat
     4201 ccggaggttg atttttaccc gaccatggat ctttgccctt ctctggctca ttccacccca
     4261 tgtactactc ctagcaattt caaatgaatt taggatttta tggggtatat gaagtttaag
     4321 caagtagata cttcaggact tgtatattga aatgaagcaa aagactatct gtacttggca
     4381 atcatctgga aatactcaag atgttttata aaccttcgca gaccgccaag gtgtctgtcg
     4441 aaggttcaag atcaaaatat tgacataaat tattttagaa caactcttgg agaattctgt
     4501 tgagactcaa tatcctcatg taccctcggt atcgggttaa acatttcatt caacacaatc
     4561 ctggtacggc tcgttggatg atttttcgaa taccgaacta ctgtatccca atcacaattt
     4621 ccttcggcga tcatccagcg catggtactc caacgattgg taatcatttg gctatcatat
     4681 gcttgcgtgt atcgcctgac aaattcggca tagagtttca gtgggttcct ctctgaacgt
     4741 agattttccg tagcagtagt tgataatgac gaaacatttt cgctggcaaa ggaaggatca
     4801 ctgtattttc tcgttactaa ttcttcaaca ataagggccg cctcactggg aacaccgagt
     4861 aaatgacgaa tagaactccc ccatgaatga tcggcataaa ctatattatg caaagcactt
     4921 tctcgtagat gacaacgctc tacaaaagca tcgaccaatt gaggcttatc tgccaaatag
     4981 gtactgaccg cttttaagag ggcaacccgg ttgctgctta acaaatttcg cattttttca
     5041 ttacggggtg gttcagcacc acggcatatt agcatggcaa tcccacgaag agcgtctgct
     5101 ttatcattct cattggccat gcgttcacat aattcttcca ctgctgtatg gccagtttgt
     5161 ttgtcttcaa ataaatgatt gttttcggat tcaagagaca aactttgagt tggtaactcc
     5221 agtaaagccg ccagtgcatt tattttgccc aaacgagcag ccaaaatgac cggagtgtct
     5281 ctcatgctac ccaggggatt tgtcaattgc gcccccatgc cggctaataa ggtaatgccc
     5341 tcagcataat ctaaaacaac agcctgatga ataggctgga aaggaacata ggctgaggat
     5401 cttaacgttg tcgcgtgtac aatgggattc ctggctaaaa tggcaattaa taattcctgg
     5461 cgtttggtat ctaatgccaa agacaacaaa gtgcgcccat aaccatcccg tttatccaca
     5521 tcaaaaaaac acctttttac gtggttaaaa gactcttcga tttttttcct tactttagac
     5581 aaaatactca aaacatccgt tttttcactc tcctcgggtg tgaaattatt ttcatccact
     5641 cccaacagat ggagtgccaa ggaaattttt ccagtaacaa ttgcctggtg caagggctca
     5701 ccaacattca catctttagc ctcgtgaagg acaaaatcta ttttggcaag caaatcccta
     5761 tttgcatcgt ctataccaag cttaggctca gagtattgtt caatcaaata atttaaaaca
     5821 gtcatttgag aaccgttcgg caaccaaaat aacttttcta aataattttt atcttcttcc
     5881 tgcttctttt gaataaattc tttaaattgc tctaattcca tttgtagaat cttattcatt
     5941 tcacttacaa aatccataat aatacttcct ttatttataa actatttata ggacaccaat
     6001 attacacaat ggacaatggg agatcaaaat attgttaact ctttttaatc ttgtcaacca
     6061 cttcctgatt caaattctca gcaatcaacc tcaacaattt aactgtatta tcgtaatctc
     6121 ttttatcaaa tatcccagca tgttggtgtg catatcgaat agggatacca atgttgacga
     6181 caggaacacc catgcctgat gcctgaacct tggagccatc ttcaccatat cctggctcca
     6241 cctcaagttg tagcgggata ttatttttat cagcaagttc cattatccag ttaattaact
     6301 cctgattggg aatcattgat ttgtcataaa caaacaagga tggtcccttc cccaaaccaa
     6361 ttcgaccttt tctatcagcc agcagcattg gataatcatc tgcgattccc acctcaacat
     6421 taattgcaat atctggatga gtcgctttgt atactgtgct ggcaccccgc ataccaactt
     6481 cctcttgcac tgtggcagca agatataatt gattgggttg ttgtttcact gctctcagag
     6541 cgtcactaat gacagctaat cctaggcgat catccaaggc cttgcctaaa aatctgttct
     6601 tgcttaattg gctaaattca cttactggtg tgacttcaag gccaggttgg attgcaaatt
     6661 ctttctgagc ttgttcttta ttctctgctc cgatatctaa gaacagggca ttgatatcag
     6721 gggaccctaa cattttctta tcacccaata aatgcggaga atccatgcca gaataagcta
     6781 acacaggtcc tttgggtgtt gaaactgtcc agcgttgagc atagatgacc gagtttgcga
     6841 tcccgcctaa agggatcact ttcaaaaagc catcaggtgt tatggattcg accataaacc
     6901 ccgtttcatc gaggtgtgcc ataaagagta atttcggtcc ttgctgatta tctttccgct
     6961 ggccgatcaa attacccata ccatcgatct ttagctccgc catatagggt tgccactgtt
     7021 tttgcaccaa atgacgaaca tttttttcgt acccagaggc accaggtacc tcagtcaatt
     7081 gggctaaaaa tgacatttca gctgctgaag ctaaaaagga aacaatcaat ccgctaataa
     7141 gagttgcatg ctttatcatt ggtaacttcc ttatttttta taaaaaccaa atcccatacc
     7201 ccatggccta gacgctcacc acgctgctca aattttgtta acggccttga gtccgggcga
     7261 ggtgagtatc cgccctcttt ttgttggtta aacaaggcgg attctgaaga taaaacattt
     7321 aaaatgtgct ccgcataatc ttcccaatca gtagcacagt gaataaatcc cgatggcctg
     7381 atttttttta ctaacatttg aataaattcg gattgaatta gtcgtcgctt atgatgacgc
     7441 tttttatgcc acggatcggg gaagaaaatt tgaaccccgg caagtgagtt ttctggtatt
     7501 ttggtttgta atacttctac tgcatcatgc actactaccc gtacattaga tatctggtgc
     7561 tcatgcaagt ccgctaccag actaccaacg cctgctttat gtacttctat gcccagataa
     7621 ttacattgag ggttattcat tgccatggca aacaacgagg tacccatacc aaaaccaatc
     7681 tctacaatag tatccgcatc atgacggcca aattcttttg caaaattcca gggagtggga
     7741 ctatcgaatt ttaattcata gtcttcaagc caaagatcta atccttgttg ctgtctgtta
     7801 ctgattcgcc cagcgcgcaa cacgtaactt ttaatcttac gttgcattac aataactcaa
     7861 tataaaattt caggattata ctgattttga ttaaatattc tacttttacc agatacctgt
     7921 ttggacaaca gccctgaaaa atcagttttt aatgaaaaca aagtaaaaat attgcaatat
     7981 ttgggttttt ttgttataaa accgctcttg tatttcatat ctggttattt tttacaacga
     8041 aagcccgcca aaataaacac cactgtagtg cattggtaag ggctgttaaa atttttttta
     8101 tcttggagta acacaatgtc tagagtatgt caggtaactg gcaagcgacc catggttggt
     8161 cataaagtgt cgcacgctaa taacaaaacc aaaagacgct tcctgcctaa cattcagaat
     8221 cataaattct gggttgaaga agaaaatcga tttgtcacct tgcggttaag tactaaaggt
     8281 atgagaatta ttgacaagtt aggcataaag gcagttctgg ataaaatcag agctaaaggc
     8341 gagaagatat aacaagggga taagccatgg cagctgttac aattaaagtg aaaatggaat
     8401 ccacagcagg aactggatac tataaaacca ctaccaaaaa tccacgtaac catcctgaaa
     8461 agatggagtt aatgatgtac gaccctaaag tacgaaagca cgtgcttttc aaagagaaaa
     8521 aagtaaaata aattgatagg cccctgtctt tggggcttac ttcctatcgt catccacttt
     8581 aaaaaccaat ttgttatatt tcaaaacaag cctatcgttt tattaaaaat ataggtaatt
     8641 ccatcacttt taaattcaaa ttaacgcccg taagcactgg ataatccttg tggatcgttt
     8701 aaactgatag ttaaagacag taaaaaaaga ggatttgaat gaaaccgaca ttgcagattg
     8761 gtattggtca acatctcacg ttaaccccac aactgcaaca agccataaga ctgctgcaat
     8821 tatccacctt tgatttacaa caagaaattc aactcgttgt tgaatcaaat cctttgcttg
     8881 aagccactcc tgttgaagaa aaagaagacg cccaaataaa taccaatcag aaccaagatg
     8941 aatattcaga ttttcaatgg tcacaactgt ataataactt aaacaagcgc aataattttg
     9001 aagaaaatga ttacaatttt gataatttac actgcactac caccaattta caagatcatc
     9061 ttaactggca acttgacctg acgcctatga gcgatattga ccgagtcatt gctacaacaa
     9121 ttattgatgc gataaacgat gacggctttt taactatacc cgtttctgaa attcacgcca
     9181 gcttaaatag cgatgatttt cctctggata tagaagaaat agaagcagtg agacacaggc
     9241 ttcaattatt tgaccccgta ggatgcgcat ctgttaattt agcagaaaca ctcgttttgc
     9301 aattggagca acttccactg gaacccaagc ttctggctac tgccaagaaa gtcattcttg
     9361 aagacattga gttgctaggg caacataact acagacaact catgaaaaat catcaaataa
     9421 gtgaaaaaac aattcatgaa atattacaag tcattcataa attaaatcca aaaccaggaa
     9481 gtttgatcca tcaagataat attgaatatg ttattcccga cttaactgtt aaaaagatag
     9541 ataatcaatg gaaagttttt ttgaatcaag atattcttcc caggctgagt atcaatagcc
     9601 aatatgccgc tttaatccaa cgcgcaaaca atagtctgga taatcaattc ttaaagcata
     9661 atttacaaga agcacgttgg tttttaaaaa gcattcaaag ccgtcaggaa acacttctaa
     9721 aagtagcaac ctgtatcatg gaataccaac atggatttct ggaatttggt gaagaagcga
     9781 tgaaaccatt aatattgaat gatgtagctt ctgccttgga tatgcatgaa tcaacggtat
     9841 caagagtgac aactcaaaaa tttattaaca ctcctcgagg tgtttttgag ttaaagtact
     9901 tcttctccag ccatgtgaat actgatagcg gaggagaatg ctcttccacc gcaatccgag
     9961 cggtaataaa aaaattaatt tcaacagaga atagaaaaaa acctttgagt gatagtaaaa
    10021 ttgcccaact aataggtgag caaggcatta aagtagcaag acgtactgtt gccaaatatc
    10081 gcgaagcaat ggggattccc ccatccaatg aaagaaaggt tattagtagt taattaatcg
    10141 gtcaacgaaa ggaggtttta tgcaaatcaa cattactgga catggtatgg atgtcactcc
    10201 tgcactaaga gcattcactg aagaaaaatt tgataagctt gagcgtcatt ttgatcacat
    10261 cagagcaatt aatgtagtat ttgatgtcga aaaagttaga caaatagcag aagcgaccgt
    10321 attggttacc aaaggtgagt tgcatgccag ttcagaatca gaagacatgt atgctgctat
    10381 tgatactttg gtagacaaat taaatcgaca actgattaaa cataaagaaa aacttttagc
    10441 ccatagagac agggattaaa tgttatttta aatacaaacc cttttagttt aatttaatat
    10501 caacaccttc tcagtgctta tgtaaactga cataaaacaa ataaacgttt tttagatgaa
    10561 gtcatagcac tgagaaagag ggttgattac taaaatcact tatttaatca ctgtacctga
    10621 tgccagagtc atgatacatt gtacgtgcta tgatcaagac caaaataaag ataatcaaca
    10681 gactcggttt acatgctcgt gcctcagcga aatttgtctc aacagcagca aaatttcaaa
    10741 gccatattga tgtaaccaaa gatactcaga ccgttaatgg caaaagcatt atgggtgtga
    10801 tgatgcttgc tgctaacaaa ggtagtgagt tgacgttgga aattgacggt ccagatgaag
    10861 aacgcatgaa tgaagcttta gtcgaattaa tcaataatcg atttggggaa agtgaataaa
    10921 gaatactaac ctctgctcca tatctttttg cgacacacag aggttcctgt tctccatcaa
    10981 tgttcctcgc ggttcttgta acatctttat tatgatcaac ctgtgattca ataaaccaac
    11041 ctaagatctc aatacaccat aattgatact aatctgaaga aatctactgt tcacaggtct
    11101 gggtaacaga tttacataat aattcatcgc gaaagacaca acccatgtta tttgctcata
    11161 tttcttcttt gtttcctcat tttatgtaat gaaatcaccg tcaacatagg gcctgacaaa
    11221 gcataaatac ctgaacttgc aaaaagcact attgcaggat tcgcagcaat tgcaacaaac
    11281 aaaagaatca tcactaaaac atataaaaat ggaaccttac ccttataatc tacttcctta
    11341 aaactgtaat agcgtaaatt actcaccatc aatacggaaa caatcaaagc caagacagct
    11401 gtaaataaag atacaatcaa atttgcccat tcattttgaa aacagatcca agcaaaagaa
    11461 gccattactg ctgcagccgg tgggcatggg agtccctgga aataacgttt atctgatttt
    11521 ccaatctgag tattaaaccg agcaagacgt aacgcaacag ccgctgtata aataaatgcg
    11581 accaaccagc caaattttcc taattgatgc agagttaaat tatataacag caaggatggc
    11641 gctactccaa aagtaaccat atcagacaaa ctatcgtatt gagcaccaaa ctcagtttgt
    11701 gtattggtta accgggcgat ccgtccatca agactgtcag cgatcattcc tataaacata
    11761 gcgacaatag cagcctcata ttgtcccttt gtggaagcca ctattgaata aaatgctgca
    11821 aataaactga cagtcgttaa taaatttggt aacaaataaa tcccagaatg attttcttta
    11881 ttcattcgca atcatacctc attcattttt aaatcaagga catgctatca taagtagaac
    11941 taaatctact atggaaactt atgcttagtc ataaagacag catgtaattg tgactaataa
    12001 ttggcaatga ctaaagcgaa acaaccattt attgcaataa aataattaat tttccctgca
    12061 ggcccagcat gatgatgata taattatcat actgtattta atatgcatag agttatgtct
    12121 aatcgaaaaa ataaaactat tgtcattgag ggtgttactt cacaaggcaa aacatttcgc
    12181 ccaagtgact gggccgagcg tatgagcggt tctttagctg tttttaaaaa tagtagaatt
    12241 tattattctc cgctcttgca accaagcgtt aatagtgagg gttataaatg tgttttactt
    12301 gatcccaaac tcaaggaatc aagcccacaa gtttatcagg caattatgga ttttgctaaa
    12361 gctaacaact tgaaaatctg tggtgaagaa gacttataac attccaaagg attgccctcc
    12421 agttttattt tctcaggact taggaaaacc cccagtttct tggttaaaaa catttttgcc
    12481 tcgatcttgg ggtttttcct aagtcctgtt tctttaaagc gcagacaaat caatcagaaa
    12541 aactagaatc ccgctgcggc ttttactgat aaagttgctt tgacaaaata atctaaagca
    12601 gaattaatca tggcaatcgt attatcaaaa ttcattgcaa aaacgcctgc aataagaaat
    12661 accaatcctt ttaatccttg ttgagtacct ggctggctgg aatgagcgac aagtacacat
    12721 ccacgtataa accacagaac acccgccgtg cgaattaaaa accccattga actgtagata
    12781 ttggcaggat tataattgga ataagttaat acattaccga caccaaaagc ggtatttgcc
    12841 ataagatcca tacccgaagg caagtagatt aaaagtgccc caactaataa atacatcatt
    12901 ggagtaaaca ttttttcctg tgaggaggat tgtgcccgat gatccccgat tttttttaac
    12961 ttgccaattg ccgttaaaaa aaataagaca cccaatatat aagctcctcc tgtgataagt
    13021 ttttgcacag gataaaggga ttggctaata ttacctaata tagtgattaa atctatcgtg
    13081 ttcatatgtt aattatatgc gcttagctct tgcaatgcat aatttttccc ctatattaat
    13141 cattttagaa taaaagttga tattccttat gcaaataaat actttcccca tcactctaga
    13201 agagtctttc atgatttctc ccaaggtaaa gcactttata ttcaattgtg aattatcacc
    13261 cccttttgaa tatttaccag gacaatttat cactattcat tttgaacatg atggaaaaaa
    13321 cttaaagcgt agttacagta ttgccaatga acccaaaaag gataatcgca ttgaatttgc
    13381 tgccgggtat ttcgaaggag ggccaggcac agaactactc tacaatttaa agccaggtga
    13441 tgtgatccac attaatggac ctttcggtcg tttgattttt aaagatgaaa ctccaggaag
    13501 atacatattg gtagcgacta gtactggtac cacaccttat agagccatgc ttaatgagct
    13561 tggtcaaaga atagaaaaac atcctgattt gcaggtagtc atccttcaag gagtgcaacg
    13621 cagcgaagag attctctatc cagatgattt tcaagccttt gcaaaaaaat atcctcaagc
    13681 aagttttcgt ccctatttaa gccgtgtgca aaaacaagac ttgaaagaca atgaatacag
    13741 tggttatgta caacatgcat ttccagagct taatttaaat ccaactcgcg atatggttta
    13801 tttatgtgga aatccaggta tgatcgacga ggcatttaat tatttaaaag aacatggttt
    13861 ttccatgcaa catattgtac gtgaaaaata tatttcacgc tgaaataccg catcaaaaac
    13921 cattgttaat cagtgtcatc ctttcggtaa ataacccgga tttgacaaaa gatgactagt
    13981 cgttatttaa atttaatagg tgccaaacca cttcgaaagg tcgacacccg ataatgagga
    14041 gataaacacg ctatgagtta tgaagaatta tcaaatacca tggagcaaat gctgcatgaa
    14101 ggaaaaggta ttttagctgc agatgaaagc aacagtacaa ttggcaagcg ttttgcgtca
    14161 atcaatacag aaaataccga ggaaaatcga agagattatc gcttgttact agcgaccact
    14221 cctgatttag aacagtatat caacggcgtc attttatttg aggaaacttt tactcaaact
    14281 gacgagcatg gaacaccaat tcctgagctt tttgctaata aaggcattat tcctggaatt
    14341 aaagtagaca aagggcttgt tgatttagcc aatacagaac atgaaaaagt tacccaggga
    14401 ttagatggtt taactgagcg tctgcttcat tttaaaaaat taggtgcaaa attcgcaaaa
    14461 tggagaaacg tttattcaat ttccgaatac acacccagcc taactgcaat caaaacagga
    14521 gctgaaaatt tgggccgtta cgctgctgct tgtcaatcag tcggcatcgt tccaattgtt
    14581 gagcctgaag tattaatgga tggcaaccat agcatagaac attgtgctga agtctgtgaa
    14641 atggttctcc atgagctgtt tcataccttg tttatccacc aagtggaatt ggagcatatt
    14701 atacttaaac cgagtatggt tacctgtggt aaagaagtga ccccatttag cgatcctgaa
    14761 gaagtggctg actacaccat tagtgtattt cgtaataatg ttccagcatc agtacccacc
    14821 attaatttcc tctccggcgg ccaaacaccc caacaggcta ccgctaactt gaatgcaatc
    14881 aatcaaattg gccaccaacc ctggttatta agtttttcat ttggaagagc attacaagag
    14941 gattgcttaa aggaatggtg tggaaacaaa gcaaatatta ccaaagcaca agaggcttta
    15001 attaagagag cccgcttaaa cagtatggct tgtttgggtg aataccaaaa cgacatggag
    15061 taaagttgca atagcgacag aaccttctct cgaccaggaa gactgtcgct ttaatcttaa
    15121 tcatacaagg cttactaaaa accccaaggt caaggcaaaa aaattttaaa tgtgggagtt
    15181 agaataaacc aaatgaccgc attaaaaatc tttttcaaca aagagactgg aggtagccct
    15241 gtcataataa ttcaaaacgt gcatacaagg gtaaatgatc agataacatc cgccaaggtt
    15301 taccctgcaa acaagcaacc tcttgaactt tcatcccgcg aaaatacacc ctgtcgacac
    15361 acaaagcagg ttttattgca ggaaatgaac gggcatgttg tccttcaagc gcgacaaaag
    15421 cttcttcaat ccctaagttt tcagcaagga ttttagaaat aacatgacgc caatcattaa
    15481 aatctcctgc cattatcata ggctcatttt taggcacaac atctttaata cgttgtatca
    15541 atgccttaca ttgctcagta cgctctgcct taaataggcc taaatgaaca cacaaaagat
    15601 gaataatatt attatccaat tttaattggg tatgaagtat acctcttgat gctctgttca
    15661 tgtttgaaag attaatactt tcaaaactct cgaacgggta tttgcttaaa atagcatttc
    15721 cgtggtggcc agattgatat actgcattct ttgcataaac gtaatgtggc catatattct
    15781 cagcaatgta ctcacattga ggggaatcag gccaactgac aattcttttt tcgcgttttc
    15841 tatgtttacc ttgcacttct tgtaaaaaaa caaagtccgg atttaatcca gcgatagcat
    15901 ctcgcatttc tggaagcaaa aaccttactg cacctactcc aaaaccttta tgaatattat
    15961 atgttatcag cgatacgctg cgcacctcag gttgcataga tactctatct ctcatttagg
    16021 tttctataat cagtataatg gtaataggat taatgtattg ctctataatt ataacccaca
    16081 ttttgaaaag aatttgtcat taaacatgat tagctcggac tctgaattat ttttatttat
    16141 cataatattg gcctttcccc aaaaaccagt aaagccattt attttgtgat cttttatagt
    16201 ttgttacaag tcaacccaat aatcgactta ctttattgct ttttgtctat ttcaattact
    16261 atcaagactt gcgaaaaaaa ccaagaccaa ggcaaaaatt gattttaatg agagagatta
    16321 aataaactaa atgactggat taaaagcatt ttttagcgaa gagattgttt ttttcgcaaa
    16381 tcctgactat tttaaaaact gaatttacat ccctgcgtat aaagtaataa gctataacta
    16441 ttacctacaa accaaagaaa attgatgaaa acaaagattt taagtatccc gggactatct
    16501 atagcatgca aagtgtgggg aaaccctgat aatccgccta ttcttgcctt gcacggttgg
    16561 ttagataatg ctaatagttt tgatgatatt gctgaacatt tacagaatga ttactatttt
    16621 atagccgtag atctgcctgg acatggtcat tcttcacact tacccccagg atgcatatat
    16681 cattttactg atggaatatt tactgttgtt gaaattatta atgccttggg actaaacaag
    16741 cttcacttgc taggacactc tatgggagcc tgcttgggaa gtcttgtagc aggggttgca
    16801 ccggatcgtt ttctttcatt aagcttaatt gaaggtttag gccctttttc tcacccggca
    16861 gaaacagcat gccagcaact gtcaaaatac ctggactatc tatcacaaaa acaatcaaaa
    16921 aaggcaaaag gctacaataa gtttgaacat gctgctcttg cccgctccgt taaaggatat
    16981 gtttctctgg atattgccaa gtcactttgc gaacgcggaa ttcaacaaga aaatggtcta
    17041 tattattgga gacacgatcg caggctatta gccccatctc cattacaaat gactgaagca
    17101 caggttcttt cgtgccttac agaaataaaa gctcaaactt accttatttg ggccagcaaa
    17161 ggattttcat ttgattcgga taagatgaaa gcaaggattc aagcagtaaa aaatattaaa
    17221 attgagaagt tagatggtgg acaccatatt catatggaaa agccagaagt catcagtcga
    17281 cttctggctg aattttatca atcaataaaa tgaattaatc gacataacaa gattgataat
    17341 ttatagtcac ttcactcaat gacttcttaa tatcatccaa attgtaaccc aaatctttgg
    17401 cagcactcaa cataccgcaa ccaccctcat caaatgttga ataaggtgtc caataatcca
    17461 tattggcctt caccataaca tcaaaagcca tacgaagatt ccaattaggt tgattagcta
    17521 atatgtagaa taaatggtta tacactccac tggagtaatg aacatctaac ccgccataat
    17581 aatcatcagc tacatcaata gacataccat cacggcttgg tttatccatg taacgcaatg
    17641 cgtcataacc actgtcttct ttcattattt ccgggccgat ctgccagctg ttttttccaa
    17701 ctgaataata ttcagcagct tgagcagcca tatctgaaaa cgactcattc atgccaccag
    17761 actgaccgaa atattcaagt ccagaatgtt gttcagtgaa tccatggcta acttcatgac
    17821 cacctacacc tagagaaacc aaaggataca tcatggtgtc accatcacca aaggtcattt
    17881 gcttgccgtc ccagtaggcg ttttcatagc cttgaccata atgcactcgc ataactaatt
    17941 gcattggcga tccatctgat ttagttaaag cctctacacc ataccaatcg tggtacatgt
    18001 gtttaatgac ataaccagca tacaatgcgt cattggtagg agaagcggct ccattatctc
    18061 tatcataacc atcagcggaa tatccggtat aataagtttt agtagattga gtatcgggag
    18121 tctctttaca agtaaattgc ataggtttgt tatttgaata gtacttatgt cccatgtcca
    18181 ctactttaac atcggtattt tccataaagc acatttcaac acttgaatca cgtgtgattt
    18241 ctaataatgg caagtccttg ccaaattgat attcaccaat tttacgattt ccaccaaatc
    18301 ccatcccttt tgcttgaact ttttccgttt ttacatcatc ccattgaaca aaaggcttat
    18361 tggtttctgc atcaataatt gctgttggtc gctcaggaat tctatcgtca tggataacta
    18421 aaacgctgac tttgtaagcc caatgtgctt gatgtttttc atcaatataa atcattggag
    18481 ttacttgatc ttcactaact tgtttattcg cgtatttatc cttaaattgt tgtaatgcca
    18541 tagaagcatt tttcacaaat gaaggtttag gctgtcctaa ttctgcttgc aacccatcat
    18601 aaatcacacc attcattttc acatcagatt ttgccgtcgc taaactagga gtggcattct
    18661 tactgtgcag gatagcgtaa ccaccaaaaa ctggaaaccc ggcatattgt tgctgcatac
    18721 gaacatgcgt cactttattc ccatcagtat gctgtcttat aaactgcaaa ctattcgttg
    18781 aaactacagc acctttcata acaccaggca aagtcaactg aaatttttgt gtgacttcag
    18841 aaaaagacga cttttgtaaa gggatcgggt cggctgcctt cacaggtgaa gcaatcccta
    18901 atgctatagc tacagctaaa ggtgacaaat aataatttgg gtgcattgat tctaactcct
    18961 taaactaaat tctcatacag caactcttcc tggagtgccc aggtttgtta tattccccta
    19021 taatttttca gttaattttt tagcgtagac taatgctcaa ctgcaaaatt aaggatgatc
    19081 caaatcagga caatacttaa gataataaaa aagtattttc cttttttaat tcaactattt
    19141 ggatagtatc ctaatatttc taggcatata accaactacc attttcaaaa tgcaagaact
    19201 cttttc
//
LOCUS       DAXLDT010000054        18850 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXLDT010000054 DAXLDT010000000
VERSION     DAXLDT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18850
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.054"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..355)
                     /locus_tag="ACF2SP_001514"
     CDS             complement(<1..355)
                     /locus_tag="ACF2SP_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2359304.1"
                     /translation="MKRLIICNDNKLTVCTQAISSGDIVEKYTPIFSLTKESDHELTL
                     ELSGIARGYYIIPSELSSSQEKAAHLITLLTRAEESQVTDMHKILNSFVSGKITSGSM
                     FNFENDGSFKREPEEA"
     gene            587..748
                     /locus_tag="ACF2SP_001515"
     CDS             587..748
                     /locus_tag="ACF2SP_001515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359305.1"
                     /translation="MQQNKTSMITLLTSWPSKETLGKPRAVYEYKKEKPSNAKLICNG
                     TMIFLFKVN"
     gene            1201..1443
                     /locus_tag="ACF2SP_001516"
     CDS             1201..1443
                     /locus_tag="ACF2SP_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359306.1"
                     /translation="MTNKILTERLNNELDELGVPCLMTERVQVCSKLFNLPKFKMEAL
                     LNGVIAIDANSIQKIADELEVNKDWLLGGIKKKTKH"
     gene            1912..2805
                     /locus_tag="ACF2SP_001517"
     CDS             1912..2805
                     /locus_tag="ACF2SP_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HFD2359307.1"
                     /translation="MFSLKKTTAAVLALGSSALFAGTMGPVCTPGNVTVPCERTAWDV
                     GITALYLQPVYDADWGYNGFTDVGGWRNWHDVDLEWDWGFKLEGSYHFNTGNDINVNW
                     YHLDADTDHWAFVDQLHAYDITWDAVNAELGQFVDFSANKKMRFHGGVQYAYIKTDVN
                     RHLNGFFLDNFNSKFNGFGPRTGLDMNYVFGNGFGVYAKSAVAILVGTSKFLDNCTVC
                     GYSYGSKNAIVPEVEMKLGADYTYAMAQGDLTLDVGYMWFNYFNALHNTAAVNVGLGA
                     SLETDFSASGPYIGLKYVGNV"
     gene            3170..4597
                     /locus_tag="ACF2SP_001518"
     CDS             3170..4597
                     /locus_tag="ACF2SP_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HFD2359308.1"
                     /translation="MPDVSGKPDANQIPDDVWLDILINYLQPQDAVNLSEVNNRLNRL
                     FATQHTQSEQLHRKKTLGILKVFTGYSSTFLFIRFNGKQRLFRAMDSNSITPLLMPIS
                     LPENVSYIESIQSTYKHTVVVGRDNRDQIIVATNKIHKDYSRQETSHNQLIPLPLRNN
                     NISLVSVGNNEESYIFNPIFLLSRNAAGQLSLQLHKINSKAPLETTIEDRDCQLPIPL
                     PDTFKTVTKISSGVWHTVIAGYDQHNRPQIATFGKNCDGQLGTGDWENRDTPTQAKLP
                     PEMHTLHTIQTRFFHTIATGLDKNGYPIVASCGYNSDGQLGCGDKDNKNALIPIKLPE
                     NMKSVEMVATGVKHTVIVGKDINNKPMVATCGHNGYGQLGTGDEENRLYLTLIGIPED
                     ITTVDYIEAGTYHTVICGRNMKNQPTLALCGCNSDGELGLAPQKITQKSSLMTNPALF
                     FPPRQDRNAINNMMKISTFSPCNLL"
     gene            4786..5370
                     /locus_tag="ACF2SP_001519"
     CDS             4786..5370
                     /locus_tag="ACF2SP_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFD2359309.1"
                     /translation="MSTSKKILMIVGDFNEDLEVYFPYQAMLMNNYLIDTVSPGKNKG
                     DFIQTAVHDLDKPHLQSYTEKLGHLFPITADFSKITLKDYDALIIPGGRAAEYQRLNK
                     DILAIIRHFHETNKPIACICHGIQILAEAGILENKKCTTVGFCEPDVRKARGHFIDTG
                     MDGVVVDGKLVTGATWLGNAPWMREFLHILQNEE"
     gene            complement(5440..7374)
                     /gene="setA"
                     /locus_tag="ACF2SP_001520"
     CDS             complement(5440..7374)
                     /gene="setA"
                     /locus_tag="ACF2SP_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SetA"
                     /protein_id="HFD2359310.1"
                     /translation="MYKIYSYLSWRIDMNIENLPQAGQEAQIDKKIHFIWVGHIMPQK
                     NIQVVSEWAEKNPGYETIIWVDKKIAPAKELDLFIQDMKSKGITVKDINEEGVCRDSI
                     RHELDQESPNYGMASDMLRLNILAAEGGIYLDSDILCSTPFPDEIHAPFGFLLSPWSQ
                     GANNALCNDIILCSKGDQITQQLADAIEQSYIARDSFEFTHEYASMKETKGERVAKTL
                     GVTGPGFLFHQLKKMGILNDKSELSAVHWELQDQRYLINGSVEAPDYFYVPQNNKNDA
                     SWVPSIKRPGIENMSFQERLENAVQLIAFDIQKTGLFNLDHYANELKVKQNSWCIAAE
                     TPAELKPDSYLLIRPRDKTGEWALYYVDEDKKLNPVTLPVIKGAIKLSELSDPLRKFH
                     IVLSQVSDPVNPTTHELKQIGRALMDLKPKQNEWQCKNKWPDVEEIAQDLWQRVTSNE
                     TLRAQIKQCSTQFESLKPRVAELGLTRVSEAETEVEEHESTVKEQEIISQKTVVEECT
                     QEKNSAQLAPENPSEEKIKTAHDLIDEIIQDVIQLDGKLGLLGGNTRQLENGKVINIP
                     NGAAMIFDDYKKYKQGELTAEGALESMIKIAKLSNKLNRHTFFNQRQPETGQFYKKVA
                     AIDLQTTIAAEYDNNHGLRM"
     gene            7610..8974
                     /locus_tag="ACF2SP_001521"
     CDS             7610..8974
                     /locus_tag="ACF2SP_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5624 domain-containing protein"
                     /protein_id="HFD2359311.1"
                     /translation="MKTLMTCIFSLIILFSPPFNPIVFAHTNNTNNQGLSASANNSGG
                     SNTPNNTYVTPQAFWNLYFDFTGDETPGYPKGKINISQTLFQSEMKKNSSLAQQNEGQ
                     LILFINSTLYIYNSDRQLELKQLMRTAPNSGFTEMTAISHIGPALMYLAKIKENGDAS
                     WKSQMENLLKDIQAVKVINAQTPNNWLEQVNAPAWKPHLTTIHNMIDYACSMAGNYMS
                     EVLNEKLSFDMASLQNDFLNGNKTYPIPYNNVMIGTFMLTALQSMDQLHSKISQLKID
                     WLHAKVIIRFVAGSNVSAGVTKGSNWLVPFVQALSNNTLAKDRIYITPYAAVKPSLGA
                     QELTQADYNYYNNTVWGARHNRTIIANEVFTNITSIFLPDRPAIPGDYTYSKPPKIED
                     FLMRLKFSLAEPTEMLSNTVGFWMAGELADKNWNYNKISIPGITTGFPQGISTYPNNN
                     PVIQ"
     gene            9064..10728
                     /locus_tag="ACF2SP_001522"
     CDS             9064..10728
                     /locus_tag="ACF2SP_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359312.1"
                     /translation="MKDFVIVGAGPIGLYWAARLAQIKESSGLPINIVVIDPRAGNYN
                     RERIVSNEAIKSISSQFSISMPSGKGIPSEAMYIRDFEKSLYEYCESKGVKFKKGTFS
                     GLSSDLVSYKDHKQSTHTVKCDMVIDCSGSNRMVLKETNRLLGKEIFEIKALGKNPHT
                     NHFSCYMSLSTEEAKKLLIPDEIDPLLQAKQLCKLRQKYDWPNFSFPVVDIRSAKNEE
                     GGVNYFIYYEVPDNLKEKDTIIQIEFLKDLLKLKYGFEDIDFTIHSFGHFPVCPKYVD
                     KPFYLGEGLPVVLAGGDCQIEPDYRKGIGIESGIERANFLFDTVHGTGKELGFLFDNY
                     YQQVARYVGYHGNLIEQFYLQRVDNIKGSSLEQAKKILCSACGSVKEIEDVAAIASEL
                     KLLGNELFKKPNYESALECYLNAIHLCQSFEKALPLTMDFVTLHSNACQTCLKLKKYE
                     QCINLANEGIKTYAEINAEDKDMLFKLLFRKASALVELGNGLDAKTQIKELDESLKAL
                     KETYELMKENSGVNNTTFVKQIESKIVNIEKKLPPPQEEVNKIEFI"
     gene            10912..12111
                     /locus_tag="ACF2SP_001523"
     CDS             10912..12111
                     /locus_tag="ACF2SP_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HFD2359313.1"
                     /translation="MLNKGYIKTTPYGLGFSELFFEENLKKYFSDPELEVLSAEYSPS
                     ASDKMAWSAKHPKQGDHPIGVQRYKISFKKNGKIQTRHVIAKSKISDKQYLQIISNAF
                     AKCGIQTEIPISDYLSQLEFSNLNRKEITIYYMQDSFEAFRHAMPLCYGSYLDEQEDI
                     CVLILECLDEEWLSLDPYNTSKWDQTAIEALIDAIASMHAVWYKKENAITEIKGFKNP
                     LNTDQMQRLMPYWNALAKAVTTSNLPFLTKEDYLFHTHLIETMPQWHIKIDAMAKTLV
                     QNDCVPKNIALCNQNGTKKVLVYDWEIATIHIPQRDIVEFLSYVLPENFDSTLLGNYI
                     ERHRVKLGETTKQPIDATEWRLGFQYTLYDYLIQRVFPQIPFEKLEARNIEKVYNNAR
                     RMMALLN"
     gene            12131..12868
                     /locus_tag="ACF2SP_001524"
     CDS             12131..12868
                     /locus_tag="ACF2SP_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFD2359314.1"
                     /translation="MAHIRVILPGYNTKVLNKEMLKPFESPGLELSVDYADIAVDAVR
                     HEFDIALMAPYLIKKVIKAEQEKVHAIVIDMMADVAMRALREAVTIPVVSLAETATHV
                     AAMMGNNFAIINTTDELTPIQESLVRSYGLYDRLACIQGIQLDPHDDYRSEKTIHGLV
                     EQCADAVIKKHADTLIFGSGRLIGYREVIQKGLEEKGLHITIIEPLPTGIYFAKFLVD
                     SKISQSKYVYRSPDASTLKTYSKILGN"
     gene            complement(12908..14221)
                     /locus_tag="ACF2SP_001525"
     CDS             complement(12908..14221)
                     /locus_tag="ACF2SP_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFD2359315.1"
                     /translation="MNSIPHLILGDIITPTQTGQSLLIKKGYVLIDGDSIIEVGEQAH
                     LNQEVSCIVSNYPDHLILPGLIDTHSHLSQYAISGSGDLPLMDWLNALVFPAEAAFAN
                     DLKRCQRHAELFMHACLGSGTTTINTMVTSSREATEVVCDVAARMGIRAFIGLVLMDR
                     NAPDSLLVDCDQAFNDLTFLKDNYHGKNNIHITVSPRFAVTCSAAMLRQAGEFARANK
                     LILHTHLDKDEGFDELIQSLFSTAHDYFDVFESTQCIADKTVFAHGTLLSLHEMKRMG
                     DYAGQVGISHCPSSNFSFAMGMAPVSFFKGLGIEVGLGSDSGGGDSLSLFNEMRSASF
                     TNKALWRLDKKTALIGAKSWLYHATLGGAKLLGLEKQIGSIEAGKKADLIILDDRNNY
                     PLAELSSLSQELTLDELQYRLARVIARSCENKLVAVYIDGKKVYQ"
     gene            complement(14403..17231)
                     /locus_tag="ACF2SP_001526"
     CDS             complement(14403..17231)
                     /locus_tag="ACF2SP_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFD2359316.1"
                     /translation="MGKTTTPEINQLNKYIIDKKSIKLANDLWIRSVPLKDTPAEKYI
                     VGTRKIPIDVAEKLQIRSLKGPIGIPEFDKNKPYNDYVVVPVCNLDDELVGLQIIQID
                     PAGNKATNLENSDFFCKKYLGPTKPLREGKAAVINKTDNVNVVFIAEGVETAASVASL
                     HEVRNNYSVLASMGVSELPATLAYVKTHYPPGATVVLLKDHDDPVENPFANSEFDKAK
                     KAFVDAGYRVIVKEPPTVKEDWNDVLAKGGVNELERQLNLNDPILCLPDIPARNSKVS
                     PHFKSLYVDLLKNEKLAEEQAICGLLDKVIGQLIDKLEKTQSSDDLHKTIKEIDEVVR
                     TTGVIRTLLSRSDSNIPKIPCQLRGLRRALKELKSLEERRRDSLRDNEDIDFSTDPKT
                     NSNGDLYKAYAYVMRHCHKHLQSLNPGDIFDLEKHSQILGFFEGRLNDIDNEIKKIPK
                     LIAEFSSDGEISRDDEVIIKQLKDKLKSLKLERKLHALEFHDIKKQLHLLRSVYPHGD
                     TPYANYYEQFIEDANSIMMKGKIEPKTIRDGLSRRWSNRKQELQEYYKKQIEAARQNF
                     VSERKKAIPPMIRYLEGLRAIVKKRSEEIDKKASLPESRDYQERYLRAMDELGQSDGM
                     DKILQGWLNNLENFKTVSPLIYYPPKEGETVDEIELIDYDSDGEETLTNLSKQIFDDK
                     ERLLEAPMNERDKGKEKREGKEGDSDPRAGVSTQALSGPSHLAEDLMQSIQPSSLKTS
                     DPQLYLTIKDFSERLAVSLYKSFEVISPKSGERQEFDGLALRKKGPTIIERKTNDGTG
                     PGLFQRNFCQQKIVSKHDFVLKNVIAELKTQSHPEEYVDIDVPDYLSWYSKNFTTEMQ
                     ERLVNAAKSTVIKALQNLTFEFNVNRPKAYSATDYRGLFFNRGLIPGGVSLRFSSLGK
                     GNEEIAHQHMDKISKERTAERESEIYFI"
     gene            complement(17447..17914)
                     /gene="sixA"
                     /locus_tag="ACF2SP_001527"
     CDS             complement(17447..17914)
                     /gene="sixA"
                     /locus_tag="ACF2SP_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFD2359317.1"
                     /translation="MKIYLVQHGDSLDKDIDPERPLSDQGQKDIQLLSNFLSLLKIEV
                     SCVYHSGKLRAQQTAESLSDHIFSKNGVESRAGLDPLDDVIPLAKEINQLKQNMMFVG
                     HLPFMNKLVSQLVAEEEHRILVSFKPGSLVCLDKADGINWCIAWMICPELLRE"
     gene            17989..18534
                     /locus_tag="ACF2SP_001528"
     CDS             17989..18534
                     /locus_tag="ACF2SP_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFD2359318.1"
                     /translation="MKPKISVFIATSLDGYIARNDGSIDWLIEANSLASPGEDCGYRS
                     FIATIDTIIMGRYSYEKVITFDEWPYGSIPVIVMSSKLIQIPEHLQHIITVSNQSPPD
                     LVSELSLQGIQHVYLDGGITIQGFLQNNLVNEMTITVIPVLIGSGRSLFGSLKNDIKL
                     THRLTTSYPGGFVQINYQIGE"
     gene            18633..>18850
                     /locus_tag="ACF2SP_001529"
     CDS             18633..>18850
                     /locus_tag="ACF2SP_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFD2359319.1"
                     /translation="MDIRYLIYAWGSSILFFIYLYLSYLVIIQRRKYHVLYLTNDDKA
                     FIARFRAHANFAEYVPLTLILMGISIIL"
BASE COUNT         5920 a         3744 c         3371 g         5815 t
ORIGIN      
        1 aagcttcttc aggttctctc ttaaatgaac cgtcgttttc aaaattaaac atgctgcctg
       61 acgtgatttt tcccgaaaca aatgagttaa gtattttatg catatccgta acttgagatt
      121 cttctgcgcg agtaagaagt gtaatgagat gtgctgcttt ttcctgagaa gaacttaact
      181 ccgaaggaat aatataatat cctctggcaa ttccagacaa ttctaatgtg agctcatggt
      241 cagattcttt tgtaagactg aaaatgggtg tatatttctc aactatatca ccagaagaaa
      301 tcgcttgtgt acatactgtt aatttattgt cattacagat aatcagtctt ttcattagtt
      361 agctcacatt ttggaaaaat aaagggcttt gattatataa aaataaaacc aaacagtaaa
      421 tatttgatct tttgtgatta cattaaaacc attagatctt acaaaaatca ataaggtcaa
      481 aagatagcat aatgaattga attaacattg atagaataga ccttttgcat aaactaatta
      541 ttttatttta ggacaaagcg cagatatttt aagaagtgga gtttatatgc agcaaaacaa
      601 aaccagcatg atcacgttgc ttacgtcctg gccttcaaaa gaaactcttg gcaaaccaag
      661 ggctgtctat gaatataaaa aggagaaacc cagtaatgcc aagctaattt gcaatggaac
      721 tatgatattt ttattcaaag ttaattaatc ctgtcctttt aaataataca atttcaacac
      781 atctcatcag taaataatgt cacttagaaa aataatctaa aaatactgac attccagatg
      841 ggagctttgc tttccgcatt caataaacat gctttaaact aaaaatgccc taaaataatt
      901 tcagttattt ctcaaaaatg gcgctgcaga tatacttcat gaagacaaca ttcgctgatt
      961 ctcagcttgt atgaaaatag ataacaatca gacgcagtgt ttgacctaaa agataataga
     1021 atctgtttta tctactcaag cgaacatccc tttcaaatta ccaaaccatt caccattgga
     1081 tttttctatt tttagagaca gggatcatgc cttcaattaa ttatctgaca aaaattaatt
     1141 tgttaatcaa tccttaagct ttagtgtttt acactgatat aaaacgagaa ggagaatacc
     1201 atgactaata aaatattaac tgaacgcttg aataatgagc tggatgaact tggtgttcca
     1261 tgtctaatga ccgagcgtgt gcaagtgtgt tccaaactat ttaatttgcc taaattcaaa
     1321 atggaagcct tattgaatgg tgttattgct attgatgcca actcgataca aaaaattgcc
     1381 gatgaacttg aggtgaataa ggattggcta ctgggcggca ttaagaaaaa aacaaagcac
     1441 tagaggctgt tgacattttt ccgacagcta acctactgcg gctttgtccg cagtatccta
     1501 caaacttgac tcaggctctc tgtatttata aaggcactaa tccatattta tgttgccaat
     1561 tttagaatag cattttctga aatggcagga gctaatgatt ttttcgaatg atatcttaaa
     1621 tctctccact atcctggctt ttatctacca ttgtaaattc cctggcatca tgtgtttctc
     1681 cacccgttat ttcaaaatcc aagggaaaag caggctaatt gttgaagtat aaatcaacaa
     1741 cggcatatac tgatcaaatc ttatatttaa tgcttaaatc aatgagttag ataatcttaa
     1801 tttaataaaa ttacctctat tatttgatga agaatcaaat tgattctgtt agaattggtt
     1861 tatcctagat gttcaaagga ataacaataa taaatcagtg gagaacagga tatgtttagt
     1921 ttgaaaaaaa caactgcggc tgtacttgct ctaggaagca gtgctttgtt tgccggtacg
     1981 atgggaccag tatgtactcc aggcaacgta actgttcctt gcgagagaac agcatgggat
     2041 gttggtatta ccgcgctcta tctgcagcca gtgtatgatg ctgattgggg ctataatggt
     2101 tttactgatg ttggtggctg gagaaattgg catgacgttg atttagagtg ggattggggc
     2161 tttaaactag aaggctctta tcacttcaac acaggcaacg acatcaatgt caattggtac
     2221 catttggatg ccgacaccga tcattgggcg tttgttgatc aattgcacgc ctacgacatt
     2281 acctgggatg ctgtgaatgc tgaactgggg caatttgtcg attttagcgc gaacaaaaaa
     2341 atgcgcttcc atggtggcgt tcagtatgcc tacatcaaaa cagacgttaa tcgtcattta
     2401 aacggcttct ttttagataa cttcaattcc aaattcaatg gatttggtcc ccgtactggc
     2461 cttgatatga attatgtatt tggtaacggg ttcggagttt atgcgaaatc agctgttgct
     2521 atcttggttg gtacaagtaa gttcctcgat aactgcactg tctgtggata ttcttatggt
     2581 tccaaaaatg caattgtgcc agaagttgaa atgaagctgg gagcagatta tacctatgca
     2641 atggcccaag gcgatttgac tctggacgtt ggttacatgt ggtttaatta cttcaacgca
     2701 ctgcataata ctgctgccgt gaatgttgga ttaggagctt cattggaaac agacttctct
     2761 gcatctggcc cctacatagg cttaaaatac gttggcaatg tctaaatgac cccaaattat
     2821 gccgctaatc tagtcatgat caagcagcag tatgcaaaaa acccgtattg acataggcta
     2881 atacgggttt ttttatttct ctctgtttaa ttaagttagt taacctatct aagctaactt
     2941 gtaattatat gatggtttat caatcaaatt ggtattattt tatactaaaa caataattgg
     3001 tagccctgtt gagcaacata accaatgaaa aatcacaatc gtgatctgct gtgcagacta
     3061 tggcaaacgc aataagctgt tgattctgcc tgatgtataa ttcttttcca ttccaaagat
     3121 cgtgggttat aatgaaatca tctgtcacca accgtttata aaaataaaca tgcctgatgt
     3181 tagcggcaaa cctgatgcaa accagattcc agatgacgtc tggcttgaca ttcttatcaa
     3241 ttatcttcaa ccacaggatg ctgtgaacct gtctgaagtc aacaatcgcc tgaaccgact
     3301 tttcgcaact caacatactc aatctgagca attgcaccga aaaaaaacat taggaattct
     3361 gaaggtattt acaggatata gttccacctt cctgtttata cgcttcaatg gcaaacaaag
     3421 attatttaga gcaatggata gcaatagcat aaccccacta ttaatgccca tctctttgcc
     3481 agagaacgtc tcttatattg aatccattca atcaacatac aaacacactg ttgttgttgg
     3541 tcgtgataat cgcgatcaaa ttatagtggc aacaaataaa atccataagg attattcaag
     3601 gcaagaaacc agccacaatc agctgattcc ccttccatta cgcaataata acatttctct
     3661 ggtatctgtc gggaataatg aagagagcta tatttttaac cctatttttc tcttatcccg
     3721 caatgcagca gggcaattga gtcttcaatt acataaaata aatagcaagg ctccattgga
     3781 aaccacaatt gaggatagag actgccagtt gcccatccca ttgcctgaca cgttcaaaac
     3841 agtaacaaaa ataagctcag gggtgtggca taccgtcatt gcaggctatg atcagcacaa
     3901 ccgacctcaa attgctacct ttggaaaaaa ctgcgatggt caattaggta caggggattg
     3961 ggaaaaccga gatactccca cccaggcaaa gcttccccct gaaatgcata cactccatac
     4021 aatccaaacc agattttttc acaccattgc cactgggctt gataaaaatg gataccctat
     4081 cgttgcaagc tgcggttata actccgatgg ccaattggga tgcggggata aagataataa
     4141 aaacgcattg attcccatca aacttccaga aaatatgaaa tcagtagaaa tggtcgccac
     4201 tggagtaaaa cacaccgtta ttgtgggaaa agacataaat aacaagccta tggttgcaac
     4261 ctgtgggcat aacggctatg gacagctggg tacaggcgat gaagagaacc gcctctactt
     4321 aaccctcatc gggattcccg aggatattac aactgtcgat tatattgaag caggcaccta
     4381 tcatactgtt atttgtgggc ggaacatgaa aaatcagccg actcttgctt tatgtgggtg
     4441 taattcagac ggtgaattgg gacttgctcc acaaaaaatc acccaaaaat catccctgat
     4501 gaccaaccca gccctgttct ttccgccgag acaagacagg aacgcaataa acaatatgat
     4561 gaaaattagt acattctcgc catgtaattt gctatgaaat ttaatttgtt tccatagaga
     4621 aaaatggagc tccagaatct cattattttt ccggaagaaa agaaccaatc ccacaaccaa
     4681 accactctct tacttatatc tgttctgata gacaagaatt tctgggcaaa gctaattaat
     4741 agctgccata cttaatgaat tcaattcgtt aaaatggaat gcatcatgtc gacttcaaaa
     4801 aaaattctta tgattgtagg agactttaac gaagatttgg aagtttactt tccctatcaa
     4861 gcaatgctca tgaacaatta cctcatcgat actgtttcac ccggcaaaaa taaaggagat
     4921 tttattcaaa ccgctgtaca tgatttggat aaaccgcatc ttcaatctta caccgaaaaa
     4981 ttaggacacc tattccccat tacagccgat ttctccaaaa tcactttaaa ggattatgac
     5041 gctttaatca tcccgggtgg aagagccgcc gaatatcaac gtttgaacaa agatatttta
     5101 gctattatca ggcattttca tgagacaaat aaaccgattg cctgcatttg tcatggcata
     5161 caaattctcg ctgaggccgg gatacttgag aataaaaaat gtacaaccgt cggtttttgt
     5221 gaaccggatg ttcgaaaagc aagaggacat tttatcgaca cagggatgga tggtgttgtg
     5281 gttgatggta aattggttac gggagcaaca tggctaggaa atgccccctg gatgagagaa
     5341 tttcttcata tcttgcaaaa tgaggaataa tcattttgca aaattattgg ctatgggcaa
     5401 tcagattaag ttgaaaccaa ttcactgttt atataaaaac tacattctta acccatgatt
     5461 gttatcgtat tccgctgcaa tggttgtttg taaatcaatt gcagccactt ttttatagaa
     5521 ttgcccggtt tctggttgcc tttgattaaa aaaagtatgc cgattaagtt tattagacag
     5581 ttttgcgatt tttatcatag attctaacgc accttcagct gttaactctc cttgcttata
     5641 ttttttataa tcatcaaata tcatagcagc gccattaggt atattaataa ccttgccatt
     5701 ttccagctgg cgtgtattcc cacccaatag acctaatttt ccatccagct gaatcacatc
     5761 ctgaataatt tcatcaatta aatcatgagc agttttaatc ttctcctcag atgggttttc
     5821 tggcgctaat tgggcagaat tcttttcttg agtgcattcc tctaccacag ttttctgcga
     5881 gataatttct tgttctttaa cagtagactc atgctcttca acttctgttt ccgcttcaga
     5941 gactcttgtt aaacctaact cagctaccct tggttttaat gattcaaatt gagtggaaca
     6001 ttgtttaatt tgtgctctta acgtttcatt tgaagtaact ctttgccaaa gatcctgtgc
     6061 aatctcctca acatcaggcc atttgttttt gcattgccat tcattttgct taggcttaag
     6121 gtccattaag gctcttccaa tttgctttaa ttcatgagtt gttggattta cagggtcaga
     6181 aacctgactt aatactatat gaaattttct gagaggatct gagagttctg aaagtttaat
     6241 agctccttta ataactggta atgtcacagg atttaatttt ttatcctcat caacataata
     6301 tagagcccat tccccagttt tgtccctggg tctaatcaac aaataagaat ccggttttaa
     6361 ctcagcgggt gtttccgcag caatacacca gctgttttgt ttcactttta attcattggc
     6421 ataatgatcc aaattaaata aaccagtctt ttgaatatca aaggctatca gttggactgc
     6481 attttcaagt ctttcttgaa aactcatgtt ttctatcccc ggccttttga tactgggaac
     6541 ccaactggcg tcatttttat tgttctgtgg tacataaaaa taatctggtg cctccacact
     6601 cccattgatt aaataacgtt gatcttgtaa ttcccaatga accgcactaa gttcactttt
     6661 gtcattcaaa attcccattt tttttaactg atgaaacaaa aatcctggcc cagttacgcc
     6721 taatgtttta gcaactctct ccccttttgt ttccttcatt gatgcatatt catgagtgaa
     6781 ctcaaaagaa tctctcgcaa tataagattg ctcgatcgca tcagctaatt gctgggttat
     6841 ctgatctcct ttgctacata aaatgatgtc attgcaaaga gcattatttg ctccctgaga
     6901 ccagggagac aataaaaaac caaaaggcgc gtgaatttca tcagggaaag gtgtggaaca
     6961 taagatatct gaatccagat aaatccctcc ctctgcagcc aagatgttta atcgcaacat
     7021 atcactcgcc atcccataat taggagattc ctgatcgagt tcatgacgta tgctatcccg
     7081 acacacacct tcttcattaa tatcttttac cgtgatgcct ttggatttca tgtcttgaat
     7141 aaaaagatcg agttctttgg caggcgctat ttttttatct acccagataa tcgtttcata
     7201 gccagggttc ttttcagccc attcggagac aacctggatg tttttttgag gcataatatg
     7261 accaacccat ataaaatgga tttttttgtc aatttgagcc tcttgaccag cctgtggtaa
     7321 attttctata ttcatatcaa ttctccaact tagatatgaa tatattttat acagattgat
     7381 attaagcccg gatgagcatc ataagaataa aactcatagt acatgcctgt taagaatagc
     7441 cagaacctaa cgagagcaat taagaaataa agaataacta caagccgatt tcccaagtcc
     7501 taacccatga gtgtcgatgg attttcgtaa cgattatcaa tataattttc cggtaaattt
     7561 gtggctttaa tttgctagga acagagccca aaccaccaag gattgaatca tgaagacctt
     7621 aatgacctgc attttctccc tcataatatt attttcaccg ccttttaacc ctatagtttt
     7681 tgctcatacc aataatacca ataatcaagg tttatcggct tcagctaaca acagtggagg
     7741 cagcaacaca ccaaataaca cttatgtcac acctcaggct ttttggaatt tatattttga
     7801 cttcaccgga gatgaaactc caggatatcc caaaggtaaa attaatattt cccaaacctt
     7861 atttcagtca gaaatgaaaa aaaacagcag cttggcgcag caaaatgaag gccaattaat
     7921 ccttttcatc aactcaacac tgtatattta taatagtgac cgtcagcttg agctaaaaca
     7981 attgatgcgt actgcgccaa attcaggatt cactgagatg actgcgatat cacatattgg
     8041 cccagctctg atgtatctcg caaaaattaa agaaaatggc gatgcaagct ggaaatcaca
     8101 aatggaaaat ttacttaagg atatccaggc agtaaaagta atcaatgcac aaacacctaa
     8161 taattggctg gagcaagtca atgcgccagc ctggaagcct catctcacta caatccataa
     8221 tatgattgat tatgcctgtt ctatggcagg caattacatg agtgaggtat tgaacgaaaa
     8281 actatcgttt gatatggctt ctttacaaaa cgactttctt aacggcaata aaacgtatcc
     8341 tatcccttat aataacgtca tgatcggcac ttttatgtta actgccttgc aaagcatgga
     8401 tcagcttcat agtaaaataa gccaattaaa aatagattgg cttcatgcga aggtaattat
     8461 ccgttttgtc gcaggatcta atgtttcagc tggcgtgact aaaggcagca attggttggt
     8521 cccatttgtt caagcactat ccaataatac attagctaaa gatagaattt atatcacgcc
     8581 ttatgccgca gtgaaaccat cgttaggtgc tcaagagtta acacaagcag attacaacta
     8641 ttataataat acggtatggg gtgcaagaca taaccgtaca atcatcgcca acgaagtatt
     8701 tactaacatt acgagcatct tcttacctga tcgccctgcc atacccggtg attatactta
     8761 ttccaaacca cctaaaatcg aagattttct aatgcgctta aaattttcat tggcagaacc
     8821 aactgagatg ttatccaata ctgttgggtt ttggatggct ggtgaacttg ctgataaaaa
     8881 ctggaattac aataaaatca gcatcccggg tattactact gggttcccac aaggcatatc
     8941 cacctatccg aacaataatc ctgtaattca atgatgaaca aagcaatcaa attatcctct
     9001 taaggtttga ttaatattct tacattataa ttactcaata taacccttga gtgaggatat
     9061 caaatgaaag attttgtcat tgttggggct gggccaatag gcttatattg ggctgcaaga
     9121 cttgcacaaa taaaagaaag ttcgggatta cctatcaata ttgtagttat cgacccaagg
     9181 gcgggcaatt acaatcgaga aaggatagta tccaatgaag caatcaaatc gataagctct
     9241 caattctcaa ttagtatgcc gtccggcaaa gggatcccca gtgaggccat gtatatccgg
     9301 gactttgaaa aatcactata cgaatattgc gaaagtaagg gtgtaaaatt taaaaaaggg
     9361 acattttccg gactaagtag cgatttggtt tcatataaag atcataagca aagcacacat
     9421 acagtcaaat gtgacatggt cattgattgt tctggttcta atcgtatggt tctcaaagaa
     9481 actaatcgct tattgggaaa agaaatattt gaaatcaagg cattgggtaa aaacccacat
     9541 accaatcatt tttcttgcta tatgtcatta agtactgaag aagcaaagaa actactcatc
     9601 ccagatgaaa tagatccctt actccaggcg aaacaattat gcaaattacg acaaaaatat
     9661 gattggccaa atttttcatt tccagtggtc gacatacgct ctgcaaaaaa tgaagaaggt
     9721 ggggttaatt attttattta ttatgaagta cccgataatt taaaagagaa agacacgata
     9781 atacaaattg aatttcttaa agatttactc aaattgaaat acggttttga agacattgat
     9841 tttacaattc attcgtttgg acatttccct gtttgtccca agtatgtcga taagccattt
     9901 tatcttggtg aaggattacc tgttgttctg gcaggtggtg attgtcaaat tgaacccgat
     9961 tatcgtaaag gaattggcat agaaagcgga attgaacgag ctaattttct atttgatact
    10021 gtgcatggta caggtaaaga actagggttc ttatttgata attactatca acaagtagcc
    10081 agatatgtag gctatcatgg caacctgatt gaacagtttt atctgcaaag agtggataac
    10141 attaaaggct cttcgcttga gcaagcaaag aaaattcttt gctctgcttg cgggtcagtg
    10201 aaagaaatcg aagacgttgc tgccattgct agcgaattaa agcttttagg caatgaactt
    10261 ttcaagaaac ctaattatga aagtgctttg gaatgttatc ttaatgctat ccatttgtgt
    10321 caatcctttg aaaaagcctt gccactgacc atggattttg tgacgttgca ttccaatgcc
    10381 tgccaaacct gcctgaaatt aaaaaaatat gaacaatgca taaatctggc aaatgaaggc
    10441 atcaagactt atgcggaaat aaacgcagag gataaagaca tgcttttcaa attattattc
    10501 cgaaaagcct cagctctcgt tgaactaggt aatggtcttg atgccaagac acaaataaaa
    10561 gaattggatg agtcattgaa agctctgaaa gaaacatatg agctaatgaa ggaaaactca
    10621 ggtgtgaata ataccacctt tgttaaacag attgagtcca aaattgtcaa tatcgagaaa
    10681 aaactccctc ccccacaaga ggaagtaaat aaaatcgaat ttatttagct ttaaattggc
    10741 aacattataa aaatgcatgg cttgctcatt gatgaataga agtctatctc acagtgaaca
    10801 agcctaatca tttctcgtag tcgattagaa agactgtctt ccctaataaa tgttcaatac
    10861 agctcaatgc tgctattctt aattgaaata ggtttagtag agggagtgaa aatgctaaat
    10921 aagggatata ttaaaacaac accctatgga ttaggatttt cagagctttt tttcgaagag
    10981 aatttaaaaa aatatttttc tgatcccgaa ttagaagtat tgtcagcaga atactctcct
    11041 tctgccagtg ataaaatggc gtggtctgcc aaacacccca agcaggggga tcatcccata
    11101 ggagtacaac gttataaaat aagctttaaa aaaaacggta aaatacaaac ccgtcatgtc
    11161 atcgccaaat ctaaaattag cgataagcaa tatctccaga ttatatcgaa tgcttttgca
    11221 aaatgtggta ttcaaacgga aatacccatc tctgattatt tgtcgcaact ggaattttcc
    11281 aatttaaaca ggaaagaaat cactatttac tatatgcaag attcgtttga agcctttcga
    11341 catgctatgc cattatgcta tggatcttat ttagacgagc aagaagacat ttgtgtatta
    11401 attttagaat gccttgatga agaatggcta tccctcgatc cttataatac ctcgaaatgg
    11461 gatcaaacag cgattgaagc attgattgat gcaattgcct caatgcacgc cgtctggtat
    11521 aaaaaagaga acgcgattac cgagatcaaa ggcttcaaaa atcctttaaa tactgaccaa
    11581 atgcaacgct tgatgcccta ttggaatgcc ttggcaaagg cggtgacaac atcaaatttg
    11641 ccttttctta caaaggaaga ttatttattc catacccatc ttattgaaac tatgccgcaa
    11701 tggcatatca aaattgatgc tatggctaaa acattggtac aaaatgactg tgtaccgaaa
    11761 aatattgcat tatgtaatca aaatgggaca aaaaaagttt tggtttatga ttgggaaatt
    11821 gcaactattc atataccaca acgcgatatt gttgagtttt tgtcttatgt cttacctgaa
    11881 aatttcgata gtacgttatt aggcaattat atagagagac atcgggtaaa acttggcgaa
    11941 acaacaaaac agccgattga tgcaacagag tggcgactcg gatttcaata tactttatac
    12001 gattatttaa tacagcgtgt ttttccacaa ataccttttg aaaagcttga agctcgtaac
    12061 atagaaaaag tgtataacaa tgcccgtcgc atgatggccc tactgaacta attttctgga
    12121 gtgattaagg atggctcata tacgtgtaat actgcccggg tacaatacta aagttttaaa
    12181 caaggaaatg ttgaaaccat ttgaaagccc tggcctggag ttaagtgttg attatgctga
    12241 cattgcggtt gatgcggtaa ggcacgaatt tgatattgcc ttgatggcgc cttatctcat
    12301 aaaaaaagta ataaaagccg agcaagaaaa ggttcatgcc atagtgattg acatgatggc
    12361 agatgtggca atgcgcgcac ttcgcgaggc agtgacaatt cctgtcgtca gtcttgctga
    12421 aacagcaaca catgttgcag ccatgatggg caataatttt gcgattatca atacaacaga
    12481 tgaactgacg cccattcagg aaagtttggt ccgtagttat gggttatatg atcggttggc
    12541 atgtatccaa ggcatacaat tggatccgca tgacgattat cgctcagaaa aaacaatcca
    12601 tggcttagtt gagcaatgtg cggatgccgt gataaagaag catgctgata cacttatttt
    12661 cgggtcaggg cgcttgattg gttatcgtga agttattcaa aaaggtttag aggaaaaagg
    12721 tttacacatc accattattg agcccctgcc taccggaatt tattttgcaa aatttctggt
    12781 agactccaaa atttcccaaa gcaaatatgt ttatcggtca cccgatgctt ccacgttaaa
    12841 aacgtattca aaaattttgg ggaattaatc atcttgattt tatctcgaat ctcattaggc
    12901 ctggtgatta ctgataaact ttctttccat caatataaac agcaactaac ttgttttcgc
    12961 aggatctggc aatcactctt gccagacgat attgaagttc atcaagggtt aactcttgtg
    13021 acaaagaaga aagctctgcc agaggatagt tattcctgtc atccaggata atcaaatccg
    13081 cctttttacc tgcttcgatg ctgccaattt gcttctctaa ccccagcaat ttagctcctc
    13141 caagagtggc atgatatagc cagctttttg caccaatcag agccgtcttt ttatcaagcc
    13201 tccataatgc tttattagta aaactcgctg aacgcatttc attaaaaaga gaaagactgt
    13261 cccctccacc actgtctgag cccaatccaa cctcaatccc caagccttta aaaaaagaaa
    13321 ccggtgccat tcccatagca aaagaaaaat tactggaggg acaatggcta attccaactt
    13381 gcccagcata gtctcccatg cgtttcattt cgtgaagtga taacaaagtg ccatgtgcaa
    13441 acacagtctt atcagcaata cattgagtgg attcaaatac atcaaaataa tcatgagctg
    13501 tgctaaacaa gctttgaatc aattcatcaa acccttcatc cttatcaaga tgggtatgca
    13561 atatcagctt gtttgcacgg gcgaactcac ctgcttgcct caacatagcc gcagaacaag
    13621 tcactgcaaa ccggggtgaa acagtgatat gtatattatt tttaccatgg taattgtctt
    13681 ttaaaaaggt tagatcgtta aatgcttgat cacaatcaac cagtaaacta tcaggtgcat
    13741 ttctatccat caacaccaat ccaataaacg ctctgatacc catgcgcgcg gccacatcgc
    13801 aaactacttc tgttgcttct cgactgctgg ttaccatagt attgatagtt gttgtgccag
    13861 aacccaaaca agcatgcata aaaagctccg catggcgttg acatcttttc aggtcatttg
    13921 cgaaagctgc ttctgcagga aaaaccaaag catttaacca atccatcaac ggcaaatcac
    13981 cagatccact aatagcgtac tgggaaagat gactatgtgt atcaattagg cctgggagga
    14041 ttaaatgatc aggataattt gacactatac atgatacttc ttgatttaaa tgggcctgct
    14101 cgcccacctc aataatgcta tcaccatcga tcaagacata acccttcttg ataagaagac
    14161 tttggccagt ttgcgtagga gtgataatat cccctaaaat gagatgagga atgctattca
    14221 attcatatcc tgagatttat ttctttctat caaaaagtat agacaacctc ttggactaac
    14281 tgaaatttgg ggtaaaaaat atcatagtaa aaagagctct ttcttttcca cttgggtaag
    14341 agagagctct tttgtaagat taacaacttg agttattgaa gcaatgagtt tttatttaac
    14401 tctcatataa aataaatctc gctttctcgc tcagctgttc tttcttttga aattttgtcc
    14461 atatgctggt gagctatctc ttcatttcct ttgccaaggc tggaaaaacg cagtgaaacg
    14521 ccacctggaa ttaaaccgcg attaaagaac aatccgcggt aatctgtcgc tgagtaggct
    14581 ttaggcctat tgacattgaa ctcaaaagtc aaattctgca atgctttaat aactgttgat
    14641 ttggcagcat taaccaatcg ctcttgcatt tccgtagtaa aattcttact gtaccagctt
    14701 aaatagtccg ggacatcaat atcaacatat tcctcgggat gagattgggt ttttaattcc
    14761 gcaataacat ttttcaagac aaaatcatgc ttggagacaa ttttttgctg acaaaaattg
    14821 cgttgaaaga gccctggacc tgtaccatca tttgttttac gctcaataat ggttggtcct
    14881 tttttccgta aagcaagacc atcaaactcc tgtctttcac cactttttgg agaaataacc
    14941 tcaaaagact tgtaaagact cactgccaac cgctcggaaa aatccttgat agtcagatac
    15001 aactgcggat cgctggtttt caggctagaa ggttgaatag attgcatcaa atcttcagca
    15061 aggtggcttg gaccactcaa agcctgtgtg ctaacacccg cccgcggatc agaatcgcct
    15121 tcttttcctt cccgtttttc ctttccttta tcacgttcat tcattggcgc ttctaataaa
    15181 cgctctttat cgtcaaagat ttgtttggac aaattagtta aggtttcctc tccatcagaa
    15241 tcatagtcaa tcaactctat ctcatccacg gtttcaccct ctttaggagg ataataaatc
    15301 aatggagaca cagttttgaa attttcaaga ttattcaacc agccttgtaa tattttatcc
    15361 attccatcag attggcccaa ctcgtccata gcgcgtaaat atcgctcttg ataatcacga
    15421 gattcgggga gtgaggcttt tttgtctatt tcctcacttc tctttttcac aattgctctt
    15481 aatccttcca gatatcgaat catgggagga attgccttct ttctttcgct gacaaaattc
    15541 tgcctggctg cttcaatttg ttttttatag tactcttgta attcctgctt tctgttagac
    15601 catctcctgg ataacccatc cctgatagtc tttggttcaa tcttgccttt catcatgata
    15661 gagttcgcat cttcgataaa ctgttcataa tagttcgcat aaggagtatc tccatgcgga
    15721 taaacgcttc tcagaagatg caattgtttt ttaatgtcat ggaactccag ggcatggagt
    15781 ttcctttcca gtttcaaact ttttagttta tctttcagtt gcttaattat gacctcgtca
    15841 tctctgctaa tctcgccatc actgctaaat tcagcaatta gcttcggaat ttttttgatt
    15901 tcattatcaa tatcatttag ccttccctca aagaatccta gaatttggct gtgtttttcc
    15961 aaatcaaaaa tatctcccgg atttaaagat tgaagatgct tatgacaatg tctcataaca
    16021 taagcataag ctttatataa atcaccatta ctattggttt ttggatcagt cgagaaatcg
    16081 atgtcctcat tatcccgtaa actatcccga cgtctttctt caagtgattt gagttctttt
    16141 agtgccctcc gaagccctct caattggcac gggattttag gtatattgct gtcggatcta
    16201 gataaaagcg tacgaattac tcctgttgtt ctaaccactt catcaatctc tttaatggtc
    16261 ttgtgcaaat catcactcga ttgagttttc tccaatttat caatcaactg cccaattact
    16321 ttatccaaaa gaccacaaat cgcttgttct tctgccaatt tttcattctt taaaagatca
    16381 acgtataagg atttgaaatg aggtgatacc ttgctatttc tggcaggaat atccggtaaa
    16441 cacagaatag ggtcattaag attgagctga cgctccagtt cgtttactcc ccctttggct
    16501 aaaacgtcat tccaatcctc cttgacagtt ggaggttctt tgacaatgac gcgataaccg
    16561 gcgtcaacga atgctttctt agccttgtca aattctgagt tagcgaaagg attctccaca
    16621 ggatcgtcat gatcttttag taaaacaacc gttgctcctg gggggtagtg agttttgaca
    16681 taggccaagg tagcaggcag ttcagatacg cccattgatg ctaacacaga atagttattt
    16741 ctgacctcat gcaaactagc cacacttgcc gctgtttcaa ctccttcggc aataaaaaca
    16801 acattgacat tatctgtttt attaataact gctgctttac cttctcgcag aggtttagtc
    16861 ggtcctaaat atttcttgca aaaaaaatct gaattttcaa gatttgtcgc tttgttacca
    16921 gcagggtcaa tttgtattat ctgcagacca acgagttcat catccagatt acaaacaggg
    16981 acaaccacat aatcattata aggtttgttt ttatcaaact ctggaatacc aatagggcct
    17041 tttaagcttc gtatctgtaa tttttcggcc acgtctatcg ggatttttcg agttcctacg
    17101 atatactttt cagcaggggt atcttttaaa ggtacgcttc ttatccataa atcattggct
    17161 aatttaatgc tttttttatc aataatatat ttattcaatt gattaatttc tggagttgtt
    17221 gtcttgccca taccgattac ctcacttaaa tgaagtgaaa attttggcat atttagctta
    17281 aggaaatatt aaaaaaacaa cacaaaatga atataaatta atcaaatgat ttgcaattgc
    17341 tggtaaaaaa tagaagtgac ttatcttata tcaaattata attgtctctg atctaaatca
    17401 tggataaaac ataggcagcg agctatgaag atgagaacag tattcttcat tctcttaata
    17461 actcaggaca aatcatccag gcaatacacc aattaatccc gtctgcttta tcgaggcaaa
    17521 caagactgcc aggtttaaat gaaaccaaaa tgcgatgttc ctcttcagca acaagctggc
    17581 tgaccagttt attcataaat ggcaaatgcc ctacaaacat catgttttgt tttaattgat
    17641 taatctcttt tgccaatggt atgacatcgt caagtggatc aagacctgcc ctggactcaa
    17701 cgccattttt gctgaaaata tgatcagaca aactctctgc tgtttgttgt gctcttaatt
    17761 ttccactgtg gtatacacag gacacttcga ttttaagcag ggaaaggaag ttgcttaaca
    17821 attgaatatc tttctgacct tgatcactca aaggtctttc aggatcaata tctttatcca
    17881 aactgtcacc atgttgaaca agataaattt tcatctcacc cttttaattc attagctaat
    17941 ttattaaagt gtagactcat ttgaaataac agataagaaa agcttgtaat gaaacctaaa
    18001 atttcagtat ttattgcaac aagtctcgac ggttatattg caaggaatga tggctcaatc
    18061 gattggctta tagaagcaaa ttctctcgcc tctccaggag aggattgtgg atatcgatca
    18121 tttattgcca ccatagatac gataattatg ggtcgatatt cctatgaaaa agttattaca
    18181 tttgatgaat ggccatatgg ttccataccg gtaatagtaa tgagctcaaa actcatacag
    18241 ataccagaac acttgcagca tatcattaca gtttctaacc aaagtccccc tgatttagtt
    18301 agcgaattat ccctccaagg cattcagcat gtatacctgg atggaggtat tactatccag
    18361 ggatttcttc aaaataatct tgttaatgag atgaccatta cagtaatacc tgttttgatt
    18421 ggttcaggcc gctctttatt tggctctctt aagaatgata tcaaactcac acaccggtta
    18481 actactagct atccgggtgg ttttgtccaa attaattacc agattggtga atagccagat
    18541 acttcagtcc tgtttactct gtaattgtaa tgccgcatgc aattgctatg ttatgatggt
    18601 atagtgccaa tttaccaata acaggataag aaatggacat tcgctatttg atctatgctt
    18661 ggggcagcag cattttattc tttatttatc tttatttaag ttacctggtg attatccaac
    18721 gtcgtaaata tcatgtgctc tatttaacca atgatgataa agcatttatt gcccgctttc
    18781 gcgcgcatgc caactttgct gaatacgttc cgctcacctt aatccttatg gggatatcta
    18841 ttatactcca
//
LOCUS       DAXLDT010000055        18812 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXLDT010000055 DAXLDT010000000
VERSION     DAXLDT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18812
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.055"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..231
                     /locus_tag="ACF2SP_001530"
     CDS             64..231
                     /locus_tag="ACF2SP_001530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359320.1"
                     /translation="MKAYEKAPTSLVGTLRYSISSILRDKDRVPLHIPRHRITLLGMN
                     STPPRPIPVPD"
     gene            complement(241..1620)
                     /locus_tag="ACF2SP_001531"
     CDS             complement(241..1620)
                     /locus_tag="ACF2SP_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5' nucleotidase"
                     /protein_id="HFD2359321.1"
                     /translation="MDTHKVFVNRIINMRKIKLIGLDMDHTLIRYNSKNFESLVYDLV
                     KERLAESFHYPEEIKKFKFNFDDAIRGLVIDSKNGNILKLSRYGAIRLSYHGTKQISF
                     SDQKKIYRSIYVDLGDPNYMAIDTSFSIAFCILYGQLVDLKDTNPDKMPSYQAIAQDV
                     QYCVDKVHSDGTLKNIIIKNLKKYVIREKEVVEGLKHFIRYGKKIFILTNSEYSYSKL
                     LLDYALSPFLDKGEHWQSLFEFVITLANKPRFFYDNLRFLSVNPENGTMTNVHGPIVP
                     GVYQGGNAKKFTEDLGVGGDEILYIGDHIYGDILRLKKDCNWRTALVVEELGEEIASQ
                     IRALPIEKKIVEAMSIKKELEQKYVDLCTRSIDESSQQYDQEIHDLQLQISTVDLQIS
                     RLLQEQNSFYNPKWERVFRAGAEESYFAYQVDRFACIYMEKLSDLLEHSPMTYFRANR
                     RLLAHDIDI"
     gene            complement(1684..2334)
                     /gene="rpiA"
                     /locus_tag="ACF2SP_001532"
     CDS             complement(1684..2334)
                     /gene="rpiA"
                     /locus_tag="ACF2SP_001532"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFD2359322.1"
                     /translation="MSELKIKAAKAAIAYIEDDMVIGVGTGSTVNFFIKELAAIKHKI
                     EACVASSKATEALLRAEGIPVIDLNSVQDLPIYVDGADEVNERGEMIKGGGGALTREK
                     IVANVATQFICIVDESKVVKRLGEFPVAVEVVPMARSFVARQIVKLGGDPEYREGFVT
                     DNGNIILDVFNLNFSTPMALEDSLNVIPGVVENGVFAKRLADKVLVASASGVNNLK"
     gene            complement(2327..3076)
                     /gene="rlmB"
                     /locus_tag="ACF2SP_001533"
     CDS             complement(2327..3076)
                     /gene="rlmB"
                     /locus_tag="ACF2SP_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFD2359323.1"
                     /translation="MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKA
                     ENAHVPIEKLSSQKMNQQFSEFTHQGVVALASALPEYGESDLVTLLERSKKPVLILIL
                     DGITDPHNLGACLRSADATGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLA
                     RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSL
                     PMMGSVESLNVSVATGVSLYEVIRQRGQFRE"
     gene            complement(3069..5249)
                     /gene="rnr"
                     /locus_tag="ACF2SP_001534"
     CDS             complement(3069..5249)
                     /gene="rnr"
                     /locus_tag="ACF2SP_001534"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFD2359324.1"
                     /translation="MSKKSKDPFYKREREKYAEPIPSREYIMEILDEYGRPMSRSKLF
                     DKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFF
                     IPDDGSEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFF
                     TDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRSQAIGRVIHILG
                     EHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLRNLPFVTIDG
                     EDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVP
                     MLPEALSNGVCSLNPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTAVGAW
                     LEQGKTDEKHQALWPMLESLYDLYHILLDTRRLRGAMDFETTETRIEFDENKKIKCIV
                     PVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELGL
                     SLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYT
                     HFTSPIRRFPDLLIHRAIGHLLDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREV
                     ITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAK
                     HRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE"
     gene            complement(5645..6109)
                     /locus_tag="ACF2SP_001535"
     CDS             complement(5645..6109)
                     /locus_tag="ACF2SP_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359325.1"
                     /translation="MRILLSFYLLLLSTGLFAESTFPDGCRAVPVQGQSVTLKAKKNK
                     LVFIHNITNLDLWITHPVTDPGASAGWTSRLQAGKWSALVVDKPAFILNCIESRPGHE
                     QQIPCENAIAVCQWKGVKIPTSNQGTFWVAEDLSLAALNAAVGGRGFVLPEK"
     gene            complement(6330..10304)
                     /gene="lem1"
                     /locus_tag="ACF2SP_001536"
     CDS             complement(6330..10304)
                     /gene="lem1"
                     /locus_tag="ACF2SP_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem1"
                     /protein_id="HFD2359326.1"
                     /translation="MKQLISIIKGQIQQLPIPDLKHLLLSHLKEDAHDLTELFCQQIP
                     AQKNQFSLNHSVPFGLPASLSVLAIVLTYAPNLLHRELSEQLLTMLDSIRETETSVDN
                     QLAERCTEQLNYYRDAKPVLIALKERGAKDLIQILEQADERSLLAFSHLAKDTDLNIL
                     IETFLVKNNKDLDLVNRNHLVQFLRQPDLAGWLTRFLDSLIDESINALSIRLLLSLIS
                     SEKAIEKLKEARACLAQYPFSIDLHAMRAVFDFYQYELKKDLFSPESHLVMQLLHDLL
                     KNTKLDKQQEFFQGLSQETVLLIIDYCIAEYNKTTGEEKQNCKNLLTRLCSAFSDSSQ
                     DFLGKIRTKINQLDPYLFDTPQLYQLASDLITKSCNSGKEPLSSSPIALLLSMPQFVG
                     VCTPKVLKQLVERYSLYTLTLNQKDYKQDLCFKEYAPYFDEINEHMAQIERNLLIHPE
                     AQSFWLDKKYSLLQFRVEISKSALLYQMECLLEENKRGDELNNKGLEVLYHYYALNYP
                     NLRSDLFLKVVNHIYSRFFTKGNNETEGRNELLHWLKQNFPLIDKLPKELDRKKEIFL
                     YNSAGKKIGFLGESNVAMTFVNEEIVLLARTGSMDINEPLYDQEGLVLGYLTEESCLR
                     SESAIHKNAAAWVLAGLSKSDLESSSFGLESLLHNVLFENAIGVLYQCESICLDANKR
                     IWLNNKLLKEIQEAPGEIPASVLYPLLDHFEDETVFELLGIIRNKNNALELFHYILNR
                     DSRRSILLNGLYESDFQRFLEHQGADVCLADYLVNYYDKPWFSEVLLRFAYHGKKYKK
                     PDLLSSALTFITKEISQDKKQETICDAVLDRLINSEACAALVLKEFLGDRTHNSIQKA
                     NNPEIGKVSQFFDRRHIVSLIAQLNKTSYWEQNAQYKLALHILANQHGRLFSETDLNP
                     DSPQAWQGKELKELACFTSRHLSKKRPFDNKHDIGYRVLGELLFRCANSGITSLFYKQ
                     KTLNRAITRLSITEPFLVRMVDKLWMPKGIRELYGKSLFFKGSWFDDQVFLQKELKDH
                     HPLLDWSHFIKQTWSKRDKQKKSPIICSYLLSYTGKKSVLSHLLQDYFNSFQGMPDYI
                     RPVSQLLRFFPQRDVSAVIYDALEAEMIKKPQLLDRKILSDMAFYYGKQLARKDTSFP
                     QADINLLTYWGQNKHYSLVKRGCELLVRDCEDKGVKKRLVSAAKEAEVEQDLSSSVQT
                     FYFGLIKFFKRLWHYGFNTENSSSKIIKFCDDPTPCPTRKKAGDFINAPVAESRTNSL
                     YLGFSERRKQLISLLATIKSSPAHFTRVVNYSDNTQTFFGEQPKKSINYNTPAITSNF
                     ESIM"
     gene            10415..10861
                     /locus_tag="ACF2SP_001537"
     CDS             10415..10861
                     /locus_tag="ACF2SP_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFD2359327.1"
                     /translation="MTIWVDADACPKMIKDILFRAAIRTNTNLILVANSYLTYPNSPF
                     IRSVLVEKGYDRADHYITSHMKAKDLVITADIPLAAEVIVKQGLAMSPRGELFTANNI
                     KQRLTLRDINEQLRSAGERTGGPSALSAREKTNFANALDRWLAKSK"
     gene            10952..11680
                     /locus_tag="ACF2SP_001538"
     CDS             10952..11680
                     /locus_tag="ACF2SP_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2359328.1"
                     /translation="MKRFMFILLIFLNFHAFGKPIIIGVPEFAPPFVSRSGGGEFYFG
                     FNIDLMNSICKIIEEECQYKGMNIQQLYTSLNAGKVDIMLAPTPIVSTGDTNYIFSLP
                     YLPSNAQVITLKANDNINTLMDLKGKKVGTLKYTLYGGLLQEHFQDFFDLVEFSKLSD
                     MALSLSNQKIEAIVLNAHAAKYAMSNSATELKMVGDQIPIGNGYGILALKNNAPLIMK
                     INKALLQIEKDGTYLEIYNTYFSD"
     gene            11907..12497
                     /locus_tag="ACF2SP_001539"
     CDS             11907..12497
                     /locus_tag="ACF2SP_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HFD2359329.1"
                     /translation="MQENFDPSTITTLHKTSIIRWKANGIEYQHKQFLQLVEENHAFN
                     FQLWHAEDKARRDDMGFEFVYQAKREIDAFNQQRNNRMEAMDEWLYKALQPENHNHCP
                     VNSESPGMIIDRLSILSLKSYHMDLQTKRQDVGVEHRMACTQKLEIIQQQLNQLALCL
                     DELLNEVRAKTRTFRVYHQFKMYNDPKLNPELYCSD"
     gene            complement(12463..14388)
                     /locus_tag="ACF2SP_001540"
     CDS             complement(12463..14388)
                     /locus_tag="ACF2SP_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359330.1"
                     /translation="MPHASDENQASKPTDLVFILAIAGRIDAQSRILAQKLDKSRSIY
                     YAYAVMDSLSSSYSMFKYFFDLFASPNDNDAMHEFLNSPAGLITIAVESVFLVTFSVL
                     ACQFENEKEDYYKKFIANAWPYFRDVMKGLKNAYKGWRSAVVAFSLIGGVDAKFLILP
                     MGVILGVFAAANRFWIRSMLEDRKTMMSENIALLMEIKKLISLSHEESFSYFKRIRYQ
                     TMQTRNLAFWAVAAGGLLDGLYLYVGVLSLAVLSPQLLMAMALICAFYTVACVITRIY
                     EEYDFQLRLLVTQTKCQLALITKELETNYAKLLLLQDKMDLDKVDQEELKRLKRLVHN
                     LITRFDDSRKLLSQQSNRTYLSSALLGMKNGLYAYGALASVLFLVGSILVMAGVSFPP
                     ALLTACIILGLVLVLSFTIHSLVSNYWHQKKQETQKQRPYERLIEWNNCLNDEFNKTE
                     LLAPEEFHKLLNDGLSLDPSPQFFFQEWFEVLRSLFSGFGKGQKFIDFAGNSLQELGE
                     DGHYRDTPVMYVLSVFSALLFGVTLALRALAKGLGRTPLAQQAKLTEDSKEASVSPEI
                     TKGVMQKTEEQRESIQSEKTRTDSPGHTDSLSLFGIFETKTKRNQPDSPSVPHSKSES
                     ALNQLIPSQNSTILGLA"
     gene            14638..15165
                     /locus_tag="ACF2SP_001541"
     CDS             14638..15165
                     /locus_tag="ACF2SP_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HFD2359331.1"
                     /translation="MRSKFVLFMLMSLLTGSLMANSEAQIYGYVEKATLIDQNLTLSA
                     KLDTGAKSASLHAVNITEIEKKGIPYLRFTVPTKTGDYSFEGEYVGKVKIKVRTSEAN
                     PGLLRTTPMKRPVVLLNIKLGDKVRTIKVNLTNRKRFLYPLLLGRDAIIDFNGAVDPA
                     LTFTTKSKSPVNNEK"
     gene            15155..16678
                     /locus_tag="ACF2SP_001542"
     CDS             15155..16678
                     /locus_tag="ACF2SP_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HFD2359332.1"
                     /translation="MKNNKRHVYGLILTLLLLGSGIFLYRHIVLDVPLTDTETINSWM
                     VESNLRFTADRNTPIKASFNIPYLPPNFAILDEYFVSRNYGVTTNLNGSNRETVWSIR
                     RGHGPQSLYYRAIFRQTDSDESSLPKPSVTKSQPLNDSQKSAVETITNQVRSTSADIQ
                     TFAQSTIKELNKRDGNAKLLVGNEFNDDNIINATILILNQSKIPAITVQGIYLNQQKK
                     ADLKSLLAVFNGKNWIYINPKTGSAGLPKEFLIWQYGNGPLFNVVGGNRAQFSLTVSP
                     TPINALSVAKSRGLEDSQLLRFSLLQLPVNVQGIYKILLTVPIGAFIILILRNFIGIK
                     TFGTFMPVLIALAFRETHVAWGITLFVIIISFGLLARFYLDQLRLLLVPRLAAILTVV
                     ILLMIFISVLCQNLSLDTGMSVALFPMVILTMTIERMCITWDERGASEAIKSGVGSLA
                     AAVISYGAMSYEPLQYLIFAFPELLLVLLSLILWFGQYRGYRLVELKRFKSLASAMK"
     gene            16675..17622
                     /locus_tag="ACF2SP_001543"
     CDS             16675..17622
                     /locus_tag="ACF2SP_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HFD2359333.1"
                     /translation="MINLFRRLKNHGVLSINQRNTDFVLRYNPRKLFPLVDDKLKTKK
                     LALKAGIAVPALYDIIETEQQIKSIETRFASYNDFVVKPARGSGGDGILVFKDKVYGR
                     YRQINGKLTTVQELSYHLSCLLSGAYSLGGSSDYAIIEKRVVVDPVFAEVSYEGIPDI
                     RIITLLGYPAMAMVRLPTRLSGGKANLHQGAIGVGVDLATGKTLGGVFQNDTIDYHPD
                     TLNPIVGIEVPYWNQILEIASSCYELTGLGYLGVDIVLDKEHGPLMLELNARPGLNIQ
                     IANREGGLKRYRTIEARFKENGKESTLDKVNFCRKEFAR"
     gene            complement(17619..18077)
                     /locus_tag="ACF2SP_001544"
     CDS             complement(17619..18077)
                     /locus_tag="ACF2SP_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359334.1"
                     /translation="MFGRLWLRLPACLPALDLSKMVLGRMPAETTFESVKHQFEQELH
                     RVGLKRFQENIHAEKLILEQLLRQREELTKKIDEECLNPALRRCANGHDVKGLFWSNM
                     QGNLAKIQSKKERVEMDEMIDWLCARRRFLKEWNNLSVDKELNCSASPTL"
     gene            18363..>18812
                     /locus_tag="ACF2SP_001545"
     CDS             18363..>18812
                     /locus_tag="ACF2SP_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2359335.1"
                     /translation="MRSKLVSFFSRSSRPIKAIPSKTWFETQLKSSGLDKKFSIDELS
                     ETQSSVRVFANKKYLPDTQTINEALTKVTAVNVSGDRSGYFQNGLPFPNEAGYFEKIP
                     AGHPELLSPIERLASSKKVVSSHSLVTASGGYPLTNPLLPYRKPIKVS"
BASE COUNT         5912 a         3992 c         3260 g         5648 t
ORIGIN      
        1 tatcaaaatg ttttctgcat agaatactta taatttatag ttgcaattga ttatttagac
       61 agcatgaagg cctatgaaaa agcaccaacc agtctggttg gcacacttcg ttatagcata
      121 tccagtattt tgcgtgataa agatcgtgtt cccttgcaca taccacgtca tagaataact
      181 ttgctgggga tgaatagcac tccccctcgt ccgatcccgg tacctgatta atagaaaaaa
      241 tcaaatatct atatcgtgag ctaacaatcg cctgttggca cgaaaatagg tcattggaga
      301 atgctcgagt aaatcggata atttttccat gtagatacag gcgaatctat cgacttggta
      361 ggcgaagtag ctctcttccg ccccggcgcg aaaaactctt tcccacttgg gattatagaa
      421 actgttttgt tcttgtaaca atcttgaaat ttgtaagtca acagtggata tttgcaattg
      481 caaatcatga atttcctgat catactgttg agagctttcg tcaatacttc ttgtacaaag
      541 atcgacgtat ttctgctcta actctttttt aatggacatg gcttcaacaa ttttcttttc
      601 aattggcaaa gcccttattt gggatgcaat ttcttcacct aattcttcaa caaccaaagc
      661 agtacgccaa ttacaatctt ttttcaatcg taatatatca ccgtaaatat gatcccctat
      721 atagagaatt tcatctccgc cgaccccgag atcttcagta aattttttgg cattacctcc
      781 ttggtatacg ccaggaacta ttgggccatg gacattagtc atcgttccgt tttcgggatt
      841 aactgataaa aaacgtaagt tatcataaaa gaaacggggt ttatttgcca aggtgataac
      901 aaactcaaat aaactttgcc agtgctcccc tttatccagg aatggggaca aggcataatc
      961 caacaacaat ttagaataag aatattctga gttggtcaga ataaatatct ttttcccata
     1021 acgaataaaa tgcttcaatc cttcaacaac ttccttttct cttatcacat attttttcaa
     1081 atttttaata attatgtttt tcagagtacc atccgagtga actttatcca cacaatattg
     1141 cacgtcttgt gctatggctt gatagcttgg cattttatcg ggattggtat cttttaaatc
     1201 aaccagctgg ccatacagga tgcaaaatgc tatagaaaaa gaagtatcaa ttgccatgta
     1261 attcggatca cccaggtcaa cataaatact tctgtaaatt tttttctgat cgctaaaact
     1321 gatctgtttc gtaccatggt aactcaatct aatagcacca taccggctta gttttaaaat
     1381 gttgccattc ttactgtcaa tcaccaaacc acgaatagca tcatcaaaat taaatttgaa
     1441 ttttttaatt tcctcaggat agtgaaagct ttctgccagc ctttccttga cgagatcgta
     1501 aactaaagat tcaaaatttt tcgaattata tcggatgagg gtatgatcca tgtccaaacc
     1561 aatcagttta atttttctca tatttataat tcgatttaca aaaaccttgt gcgtatccat
     1621 ttttgaggaa cctataaaat tattggcaat taatgttttg agatatgaac gtatgcaagt
     1681 cgactatttt aaattattca caccacttgc tgaagccact aacactttat ctgccaaacg
     1741 tttggcaaac actccatttt caacgacacc cgggattaca ttaagactgt cttccaaggc
     1801 cataggtgtt gagaaattca gattaaacac atccagaata atattaccat tatcagtaac
     1861 gaaaccttct ctgtattcag ggtctccacc taatttaaca atttgtcgtg caacaaaact
     1921 acgggccatt ggaacgactt ccacagcaac aggaaattcc cctaaacgtt tcacaacctt
     1981 ggattcatcg acaatacaaa taaattgtgt ggctacattc gctacaattt tctcccgagt
     2041 cagagctcct ccgcccccct ttatcatttc accgcgttca ttgacttcat cagcgccatc
     2101 aacataaata ggtaaatcct gtactgaatt taaatcaatt actgggatac cttctgcccg
     2161 aagcaatgcc tcggttgctt ttgaactggc tacacaagcc tctatcttgt gtttgattgc
     2221 tgccagttct tttataaaaa aattaactgt agagcctgtt ccaacaccta taaccatatc
     2281 atcttcaata taagctatag ctgctttggc tgctttgatt tttaattcac tcacgaaatt
     2341 gccctctttg cctaattact tcatacaatg aaacaccagt cgccactgac acatttaaac
     2401 tctctacact tcccatcata ggcaaagaaa acaatccatc gcaatgctcg cgagtcaatc
     2461 ttcttaaccc ggtcccttct gctcccatta caagggcgat gtttccagag caatccattg
     2521 cataaagtaa atgctctgct tcgccagctg ctccaaagat ccagacaccc tgttgtttta
     2581 agatgtccat agctcttgct aaattagtta ctctcaccaa aggaattgat tcagcagcgc
     2641 cacaagccac tttactgact acgggagtaa tattcgcact tttatcttta ggaatcacaa
     2701 caaaatcaac accggttgca tcagccgacc gcaagcatgc acctaaatta tggggatctg
     2761 taatgccatc cagaataaga atcaacacag gctttttact tctttctaat aaagtaacca
     2821 aatcactttc accgtattcg ggtaatgccg aagcgagggc aacaacgccc tgatgcgtaa
     2881 actctgaaaa ctgctgattc attttttgcg agcttagctt ttcgatgggg acatgggcgt
     2941 tttctgcctt gaccaaaaac tcatgaagtc ttttatctat tctatcttga ttaacaaaga
     3001 gttttttgat cattcggtgc gggtttgcta ataatgctga aactgcatgc aacccgtaaa
     3061 catactgctc attcattaca aacttcatcc tcgacaggct caaaatctat tttacgttcg
     3121 tttaaatcaa cacgagctac caacacagtc attttatctc ccaaacgata aacttgaccg
     3181 ccgcgagccc ctattaaacg atgttttgca gaatcaaacg tataataatc gttttttaaa
     3241 gaagtgacat gaaccaagcc ttccacataa atttcatcaa gctccacaaa aataccaaag
     3301 ctggtcacag cggatattcg acccttaaat acctgcccca atttgtcttg catatactcg
     3361 cattttagcc aggtaatcac ttcccgggtc gcttcatcag cacggcgctc cgtcatcgag
     3421 gaatgtttac ccaaacgatt catatcttca tggctatact caaattcata aacaggatga
     3481 ttatccagta aatgccctat tgctctgtga attaatagat caggaaatct tctgattggt
     3541 gatgtaaaat gcgtgtaagc ggaataggcc aaaccaaaat gaccttcatt cgattcaacg
     3601 tactgagcct gttttaaaga acgcagcatt accgtttcaa taagatgctt ctctggtcgg
     3661 tcaccaatca tgttcatggt acgctgaaaa tccttgggtc caggttttct cccccctccc
     3721 aatgacaatc ccagctcccc taaaaactgc ctcaatgcag taattttatc ctcttcagga
     3781 gccgcatgaa ctcggtataa agtaggaatt tcagcctttt ctaaaaatct tgctgttgca
     3841 acattcgctg ccagcatgca ttcttcaatt aatttatgag cgtcattacg tattacaggc
     3901 acgatacatt ttattttctt attttcatca aattcgatac gagtttccgt agtctcgaaa
     3961 tccatagcac ctctaagtct acgggtatcg agcagaatat gatataaatc ataaagagac
     4021 tcaagcatag gccacaaggc ttgatgtttt tcatctgtct tgccttgttc caaccatgcc
     4081 ccaactgccg tgtaggtcaa acgagcatga gaatgaatca ctgcccgata aaagcgggat
     4141 cgggatattt ttccttcttg ggtaatcgat aactcagcca ccatacataa gcgatcaaca
     4201 tggggattta atgagcaaac accattggat aaggcttctg gtagcattgg caccacttta
     4261 ccgggaaaat aaactgaatt tcctcgtctt gctgcttctt gatccaaagc agaatccttc
     4321 gccacatagt gactgacgtc tgcgatagcg acatacaatt ggaagccgcc tttgggcttt
     4381 ttgtaacaat acacagcatc atcaaaatct tttgcatctt ccccgtcaat ggttacaaaa
     4441 ggaagattac gcaaatcggt tcgacccttt aattgttcct cggaaatctg ttgtggaatt
     4501 ttacccactt ccgcaagaac atcatctggc cattcgaaag gaatgccgtg ggcatgaatt
     4561 gctacctgaa tttccatacc aggagccata tgctcaccca aaatatggat tactctgcct
     4621 atagcttgcg aacgcttgtt aggatatgcg attaactcaa ccagtactat ttggccattt
     4681 ttggcctcgc caatgaattc ttggggaatt gaaatatcct gagtcagacg tttgctatca
     4741 ggaatgacaa aattcacgcc atgatcagta aaaaatcgac ctacaaccgt ggtattcgca
     4801 tgttccagga cttcgtggat tttagcttcc ggccttcctc ttctatccaa tccagattga
     4861 taggcaagca ccatatctcc atgcatgaca gccctcattt ctttagcaga gagcaccata
     4921 tcctcggaac catcatccgg gatgaaaaat ccaaacccat caggatggcc ttgaaccgtt
     4981 ccacgcaata aattaatttt ttgtaataaa caaaagcgtc cacgcctgtc ctgcatgatt
     5041 tggccatcac gcaacatggc cttcaagcga aaacccaagg actcctgctg gctctcagag
     5101 ctcaaatcca atttatcaaa cagtttactg cgagacattg gcctaccgta ttcatccagg
     5161 atttccatga tgtattcccg gcttggaatt ggctcggcgt atttttcgcg ttccctctta
     5221 taaaagggat ctttggattt tttactcact tttcaccatt tttctcttca aacctaatgc
     5281 taactaatac ttcttaagtc ctgttctacc atttatcact ataactagca agaatatgct
     5341 gccaactatc aaagaattct gtataaaacg ccttttattc atcttttttg caatcttaca
     5401 aagcaagaag tcctcataat aatatgaggc ttttttatat tggataatct caatccattt
     5461 agcccattat taaagtttta aattttcata aggactcaag agtgggcagc atggcagcta
     5521 gctaaacaat ttgccaaagg ctcaggatag cctcatcagc ggctatcctg atttgtaatg
     5581 agaattgata tgactttttg cagcatagag tccatagcca tgccccaaga tattcattga
     5641 taaattattt ctccggcaat acaaagcctc ttccaccgac tgcggcattt aatgctgcta
     5701 atgacaggtc ttccgctacc cagaaagtac cttgattgct tgtcggtatt tttactcctt
     5761 tccattgaca tacggcaata gcattttcac aaggtatctg ttgctcatgc cctggccttg
     5821 attctatgca atttaaaata aatgcaggtt tgtccactac caaagctgac catttaccag
     5881 cttgcaatcg gcttgtccaa cccgcactgg ccccaggatc tgtaactgga tgagtaatcc
     5941 atagatccag gttggtaata ttgtgaataa acaccaactt atttttcttg gcttttaaag
     6001 tcactgattg accttgtaca ggaacagccc gacaaccatc aggaaaagta gattctgcaa
     6061 aaagccctgt tgaaagcaat aaaagataga aagagagcaa aattctcatg attcacccca
     6121 tagtgtttat gtaaccaaaa attattaaat cttcactaat aaacccatca tcaatatcgg
     6181 catcgattta agaaaacact catataatat ccctactaat ataagacttc cattgataag
     6241 tcatgcatcg ggttgattaa tgcattcaga acgcctggct aataaaacaa aaatatgagc
     6301 gaaggtataa atgccctata aggataatcc tacattatac tttcaaagtt acttgttatt
     6361 gcaggagtat tatagttaat ggactttttg ggttgttctc caaagaatgt ttgagtattg
     6421 tctgaataat ttacaactcg tgtgaagtgc gccggactac ttttaatagt agctaaaaga
     6481 ctaattaact gcttgcgtct ttcagaaaag cctaaataca aagaattggt tctgctttct
     6541 gcaaccggtg cattgatgaa atcccctgct tttttacgag tagggcaggg agtgggatca
     6601 tcacaaaact ttataatttt tgatgagctg ttttcagtat taaaaccata atgccataat
     6661 cttttaaaaa atttaattag cccaaaataa aatgtttgca cactggagct caagtcctgt
     6721 tctacttccg cttctttggc agcactaacc aatcgttttt ttactccttt atcctcacaa
     6781 tccctgacta atagttcaca tcctcttttt actaaagaat aatgtttatt ctgcccccaa
     6841 tacgttaata aattaatatc agcctggggg aaacttgtgt ccttcctggc caactgtttt
     6901 ccataataaa aagccatgtc acttaaaatt ttcctgtcta ataactgagg tttttttatc
     6961 atttcggctt ccagtgcatc atatatgact gcggacacat cgcgttgagg gaaaaatctc
     7021 aataattgac tgactggcct gatgtagtct ggcattcctt gaaaagaatt aaaataatcc
     7081 tgtaataaat gcgacaatac gcttttctta cctgtataac tcagcaaata agaacatatt
     7141 atgggtgact tcttctgttt atccctttta ctccaggttt gcttaataaa atgactccag
     7201 tccaacagag gatgatggtc ttttaattcc ttttgcaaga agacttgatc atcaaaccag
     7261 gaacccttaa aaaacaaaga ctttccataa agttccctga ttcctttagg catccataat
     7321 ttatccacca tcctgactaa aaagggttct gtgattgaca atcgggttat tgcgcgattc
     7381 aacgtttttt gcttgtaaaa taaagacgtt atccccgaat tggcgcacct gaaaagaagc
     7441 tcgcctaaaa cacgatatcc aatatcatgt ttattatcga aaggacgttt tttagataaa
     7501 tgcctgcttg taaaacaggc cagttctttt aattctttac cttgccaggc ctgtggagaa
     7561 tcggggttca aatcagtttc agaaaaaagg cgtccatgtt gattagccaa aatatgtaaa
     7621 gccaatttat actgcgcatt ttgctcccaa taagaagtct tatttaactg tgcaataagg
     7681 gacactatat gccgcctatc aaaaaattgg cttactttgc caatttccgg attatttgcc
     7741 ttctgaatgg agttatgagt tctatcacca agaaactcct ttaacactaa tgcggcacat
     7801 gcttcagaat ttattaaacg atccagtaca gcatcacaga ttgtctcttg cttcttatct
     7861 tgagaaattt ccttggtaat gaaagtcaag gcactactta gaagatctgg ctttttatat
     7921 tttttaccat ggtaagcaaa tcgtagtaag acttctgaaa accagggttt atcataataa
     7981 ttaaccaaat aatcagctaa acaaacatca gcgccttgat gttccaaaaa tctttgaaaa
     8041 tcagattcat acaagccatt cagtaaaata gaacgccttg agtctcggtt taaaatataa
     8101 tgaaaaagct caagcgcatt gtttttattc ctgatgatgc ctaataattc aaatactgtt
     8161 tcatcctcaa aatgatcgag caatggatat aaaacagatg cagggatttc tcctggagcc
     8221 tcttggattt cttttaataa tttattattc agccatatac gcttattggc atcaaggcaa
     8281 atagactcac attgatataa aacccctatc gcgttctcga ataacacatt gtgtaacagg
     8341 gactccaatc caaacgagga actttccaaa tcacttttgg acaaaccagc taaaacccag
     8401 gcggcagcat ttttgtgaat ggcactttcg cttcttaaac aactctcttc cgttaaatac
     8461 cctagaacca aaccctcttg atcatataaa ggctcattga tatccataga gcccgttcga
     8521 gctaataata caatttcctc attaacaaaa gtcattgcaa cattagactc accaagaaat
     8581 ccaatcttct tcccggcaga gttatataaa aaaatttctt tttttctgtc taattctttg
     8641 ggtaacttat caattagagg aaaattttgt tttaaccagt gaagtaactc gttccgacct
     8701 tcggtttcat tattcccctt agtaaaaaac cgtgaataaa tatgatttac cactttaagg
     8761 aataaatccg aacgtaaatt gggataattc aaggcgtaat aatgatataa cacctctaat
     8821 cccttattat ttagctcatc tcctctcttg ttctcctcca gtaaacactc catttgataa
     8881 agcaaagcac ttttactgat ctcaactcta aactgcaata agctgtattt tttatccagc
     8941 caaaatgact gagcttccgg atgaataagc agattacgct caatctgagc catatgttca
     9001 ttgatttcat caaaatatgg agcatattct ttaaaacaaa ggtcttgctt atagtccttc
     9061 tgattcagag ttaatgtata caagctgtat ctttcaacta attgttttaa aaccttaggc
     9121 gtacaaaccc cgacaaattg tggcatggat agcaataaag cgattggaga actggataac
     9181 ggctcctttc ctgagttaca tgatttagtt attaaatcgg atgccagctg atatagctga
     9241 ggggtatcaa agagataagg atccagctga ttaatttttg ttcttatttt ccccaaaaag
     9301 tcctggctgg aatcagaaaa agccgaacat aacctggtaa gcaagttttt acagttctgc
     9361 ttctcttccc cagtcgtttt gttatattcc gctatacaat aatcaatgat caaaagcaca
     9421 gtttcctgac ttaagccctg aaaaaactct tgttgtttgt ctaatttggt attttttaat
     9481 aaatcatgca gcaactgcat gactaaatgg ctttcaggag aaaataaatc tttttttaat
     9541 tcatactgat aaaaatcaaa cacagcccgc atggcgtgca aatctattga aaaaggatac
     9601 tgagcaaggc aggctctagc ctccttcagt ttctcaatcg ctttctcaga agaaatcaaa
     9661 gacaacaaca atcgaattga gagagcattg atagattcat cgattaatga atcaaggaaa
     9721 cgggttaacc atcctgccaa atcaggttga cgtaaaaatt gtaccaaatg gtttctattg
     9781 actaaatcca aatccttatt gtttttaact aaaaaggttt caattaaaat atttaaatcg
     9841 gtatctttag ccagatgaga aaaggctaac aaactgcgct catcagcttg ctccaagatc
     9901 tgaattaaat ctttggctcc tctttctttc aacgcgataa ggacaggttt tgcatcacga
     9961 taataattca attgctccgt gcatctttct gctaattgat tatcaacact ggtttcggtc
    10021 tctcttattg aatctaacat tgtcagcaac tgctctgata actctcggtg tagcagattg
    10081 ggagcataag tcaaaacgat tgcgagtaca gataacgaag ccggtaaacc aaatgggact
    10141 gagtgattaa gcgagaattg atttttctga gccgggattt gttggcaaaa gagttctgtc
    10201 aaatcgtgag catcttcttt aaggtgactt aacaacaaat gcttcaggtc gggaatgggg
    10261 agctgttgaa tttgtccttt tattatactt attaactgct tcattacgac acttccctaa
    10321 catcttaaaa taatcctttc cccaataggg atttaaaagg ataatgtttt tactataccc
    10381 ttaaactatt gatttgaaaa ctttaggata gcaaatgacg atctgggttg atgccgatgc
    10441 ttgcccgaaa atgataaaag atatcctctt tcgcgcggcc atacgaacca ataccaacct
    10501 tatcctggta gctaattcct accttactta tccaaattca ccgtttattc gctcagttct
    10561 tgtagaaaaa ggctatgacc gagcagatca ttatattacg agtcatatga aagctaaaga
    10621 tttggtgatc acagcagata ttcctttggc agcagaagta atagttaaac aagggttagc
    10681 aatgagccca agaggagaac tgtttactgc aaataatatt aaacagcgtc tgactcttag
    10741 agatatcaat gaacagttgc gttcagctgg agagcgcaca ggaggcccct ctgcattaag
    10801 cgcgagagaa aaaaccaatt ttgccaatgc tctggatcgt tggcttgcaa aaagcaaata
    10861 aaaacatcaa ttaagaaaca cattggtttt aatttgacaa aatcgattgc tcctcctaaa
    10921 ttttaagtac atcctaaatg gacttaaaaa catgaaacga ttcatgttca ttcttttgat
    10981 ctttttaaat ttccatgcct ttggaaaacc aatcattatt ggcgttccag aatttgcgcc
    11041 tccatttgta tcaagatctg gtggaggtga gttttatttc ggattcaaca tcgatttgat
    11101 gaactcgatt tgcaagatta ttgaggaaga atgccaatac aagggcatga atattcaaca
    11161 gttatatacc agtctcaacg caggaaaggt tgatattatg ctggcaccta ctccaattgt
    11221 atcaactggt gacactaatt atattttcag tcttccctat ttaccgagta atgcgcaggt
    11281 aataaccctc aaagctaacg acaacatcaa taccttaatg gacctgaaag gtaaaaaggt
    11341 aggtacttta aaatacactt tatatggtgg cctcctccaa gaacattttc aggatttttt
    11401 tgacctggta gaattttcca aactgtccga tatggctctc tcattatcaa accaaaaaat
    11461 cgaagccatt gttcttaatg cgcatgcagc gaaatatgcg atgagcaatt ccgcgactga
    11521 gttaaaaatg gtaggagatc aaatccctat aggtaatggc tatggaatac tagccctcaa
    11581 aaataatgcc ccgctcatca tgaaaatcaa caaggcttta ttacaaatag aaaaagacgg
    11641 aacctatttg gaaatatata atacctattt cagtgattag gatattatcc tgagcaattc
    11701 accgggcatt tcaaaaaccc gaaactagat ccaactccgg aattcaggtt aaactaatac
    11761 caattgaacc tgtctctcat aatgtattga gcctcaatgc atgagatata gttcaaaaca
    11821 gtcgagttca agttatgaaa acaggtatat tttcatagac taaactcatc ttaaagtaca
    11881 atccaaatta atctgcagga tatgacatgc aagaaaattt tgatccatca actatcacta
    11941 ccctccataa aacgagcatc atacgctgga aagccaatgg catagagtat caacataaac
    12001 aattccttca acttgtagaa gaaaatcatg cttttaattt ccaattatgg catgcagaag
    12061 acaaggcgcg ccgagatgat atgggctttg aatttgttta ccaggccaaa agagaaatag
    12121 acgctttcaa tcagcaaaga aacaaccgca tggaagcaat ggatgagtgg ctgtataaag
    12181 cattacagcc tgaaaaccat aatcattgcc ctgtcaattc cgaatcacct gggatgatta
    12241 ttgacagatt atctatttta tcactgaaat catatcatat ggatttacaa accaaacgcc
    12301 aggatgtagg cgtagaacat agaatggcct gcacacaaaa actggaaatc atacagcaac
    12361 aattaaatca attggctttg tgtcttgatg aattacttaa tgaggtacga gctaaaacac
    12421 gcacattcag agtttaccat caatttaaga tgtataacga tcctaagcta aacccagaat
    12481 tgtactgttc tgactaggta ttaattgatt taatgctgac tcgcttttcg aatgaggaac
    12541 tgatggagag tcgggttgat ttcttttggt tttggtttca aatataccga ataaagacag
    12601 agaatccgta tgccctgggg aatcagtccg agttttttca gattggattg attccctttg
    12661 ttcttcggtt ttctgcataa ctcccttcgt tatctcaggg ctaaccgacg cttctttgct
    12721 gtcctccgtc agcttggcct gctgcgccaa gggagttcgc cccaatcctt tggctagagc
    12781 tcttaaagcc agagtaaccc caaacaataa ggcactgaag acagataaga cgtacataac
    12841 aggcgtatca cgataatggc catcctcgcc cagttcctgt aacgaattcc ccgcaaagtc
    12901 aataaatttt tgccccttac caaacccgga aaacaaggaa cgcaaaactt caaaccattc
    12961 ttgaaagaaa aattgcggtg aagggtctaa agacaatcca tcatttagta atttatgaaa
    13021 ttcttcagga gccaacagct cagtcttgtt aaattcgtca ttaagacaat tattccactc
    13081 gatcaatcgc tcatatggac gttgtttttg agtttcctgc ttcttctgat gccaataatt
    13141 actcaccaaa gaatgaatag tgaaactgag cactaatacc agacctaaaa taatacaagc
    13201 tgtaagcaag gcaggaggaa aagaaacacc tgccattact aaaatagaac ccactaaaaa
    13261 caaaacactt gcaagcgcac cataagcata taatccattt ttcataccca acaaagcaga
    13321 tgataaataa gtgcggttcg attgttggga taataactta cgactatcgt caaaccgggt
    13381 tatcaaatta tggactaacc tttttagtcg tttgagctct tcctgatcaa ctttgtccag
    13441 atccattttg tcttgcaata agagtaattt ggcgtaatta gtttccagtt ctttagtaat
    13501 taaagccaat tgacatttgg tttgcgtcac taataatctc aactggaaat catattcctc
    13561 ataaatacgg gtaataacac aagccactgt ataaaaagca caaataagcg ccattgccat
    13621 aagcaactgc ggtgacaata cagccagact caatacacca acgtagagat ataaaccatc
    13681 aagaagtccg cctgcagcaa cagcccagaa tgcgagatta cgagtttgca tggtctggta
    13741 tctgattcgc ttgaaataag aaaagctttc ctcatgagat aaagaaatta atttttttat
    13801 ttccatgaga agcgctatat tctcactcat cattgtttta cgatcttcca gcatgctacg
    13861 tatccaaaac ctgttggctg cagcaaatac ccccaaaatc actcccattg gaagaataag
    13921 aaattttgca tccactcctc caattaaact gaatgcaaca acagcgcttc tccagccttt
    13981 gtaagcattt ttcaagccct tcatgacatc acgaaaatat ggccaggcat tagcaataaa
    14041 ttttttatag taatcttctt tttcattctc aaattgacag gccaaaacag aaaaggtaac
    14101 taaaaatact gactcaactg caatggtaat aagaccggct ggagaattta agaattcatg
    14161 catcgcatcg ttatcattag gagatgcaaa cagatcaaaa aaatatttga acatgctgta
    14221 cgaggaactt agagagtcca taacagcgta ggcataatag atactgcgac ttttatcgag
    14281 tttttgagcg aggattctac tttgcgcgtc gatacgacca gcaattgcta aaatgaaaac
    14341 taaatcagta ggcttggaag cctgattttc atcactcgca tgaggcatgt ataggtatgg
    14401 ggctatcagc taagattggg cgctattgta tgcataatta gacttgatgt caattaaact
    14461 ttcatcctga gttaaaaaaa tacaacaatg acctcaaacc cttcataata acacatccta
    14521 ttagcaaaat tattaaaaaa aataaaaaaa attgcattaa attctctctc agactgtgac
    14581 ttatgcctta aacctgctac gatattgcac caaaataaaa cccaatatga ggattaaatg
    14641 agatcaaaat ttgttctctt tatgttaatg tcactactga caggatctct tatggcaaac
    14701 agtgaagctc aaatctatgg ctacgtagag aaagccacat taatcgatca aaacctcacc
    14761 ttatctgcaa agcttgatac tggcgctaaa tcagcatcat tacatgccgt gaatatcaca
    14821 gaaattgaaa aaaaaggcat tccttattta cgttttaccg ttccaaccaa aaccggtgat
    14881 tactcatttg aaggagagta tgtcggtaaa gtaaagatta aagttaggac aagtgaggcc
    14941 aatccaggat tattaagaac aactcccatg aagcgaccgg tagtcttact taatatcaag
    15001 cttggtgata aagtcagaac aattaaagtg aacttaacta acaggaaacg attcctttac
    15061 cctttattgt tgggaagaga tgccattatt gattttaatg gggccgttga tcctgccctt
    15121 actttcacaa ctaaatccaa gagcccagtg aacaatgaaa aataacaaac gtcatgttta
    15181 tggtttgatc ctcactctat tgcttttagg atcaggtatt tttttatata gacatatcgt
    15241 tctcgatgtc cctctgaccg atacggaaac gatcaacagt tggatggttg aatccaatct
    15301 acgttttacc gctgatagaa atacgccgat aaaggcaagc tttaatatcc cttacctgcc
    15361 ccccaatttt gccattctcg atgaatattt tgtgtccaga aattatggtg tcaccaccaa
    15421 tttaaacgga tccaatagag aaaccgtatg gtctataagg cgtggtcatg gtcctcaatc
    15481 actttattat agagcaatct tccgccaaac ggacagtgat gagtcttctt tgcctaaacc
    15541 ttcggtaact aaatcacaac ctctgaatga cagtcaaaaa tcggccgttg aaacgattac
    15601 caatcaagtg agatcaacat cagcagacat tcaaacattt gcccaaagca ctattaaaga
    15661 attaaacaaa agagatggta atgcgaagct tttagttggg aacgaattca atgatgacaa
    15721 tattatcaat gccaccattt tgattctcaa tcaatcaaag attccggcca ttacagtcca
    15781 ggggatttat ctaaaccaac aaaaaaaggc tgatttaaaa tccctgctcg ctgtgtttaa
    15841 tggaaaaaac tggatttaca ttaaccccaa aactggcagt gccggcttgc caaaagagtt
    15901 tctgatttgg caatatggga atggccctct atttaatgtt gttgggggta atagagctca
    15961 attttcatta actgtatcgc caacacccat taatgcctta agtgtggcca aatcaagagg
    16021 gctagaagac tcgcaactct tgcgcttctc cttactccag ctacctgtca atgtacaagg
    16081 gatctataaa atcttgctaa ctgttcctat tggagcattc atcattttaa tactgcgtaa
    16141 ttttataggc attaaaactt ttggtacgtt tatgcccgtc ttgatcgccc tggcctttag
    16201 agagactcat gtagcatggg gcattacttt atttgtcatt ataatttctt tcggactatt
    16261 agcccgattt tatctggatc aattaagact actgttagtc ccaagacttg cagccattct
    16321 cactgtagtg attctattaa tgatctttat cagtgttcta tgccaaaatc ttagtcttga
    16381 caccggtatg tctgtcgcat tattccctat ggtcatctta accatgacta tcgagcgcat
    16441 gtgcatcaca tgggatgaaa gaggggcttc cgaagcaata aaatcaggag ttggtagtct
    16501 tgctgcagca gttatttcat atggggcaat gagttatgag cccttacaat atttgatctt
    16561 tgcttttccc gaattacttt tggtgctttt gtccttgatt ttatggttcg gtcaatatcg
    16621 tggctatcgt ttagtcgaat taaaacgctt taaatcactt gcgagtgcga tgaaatgatt
    16681 aacctgttta ggcgcctgaa aaatcatggg gttttaagca ttaaccagcg gaatacggat
    16741 tttgtattac gatacaatcc aagaaaatta tttcctttgg ttgatgacaa gctcaaaacc
    16801 aaaaaactgg ccttaaaagc tggaattgca gttcctgcgt tatacgacat cattgaaact
    16861 gagcaacaaa tcaaatcaat agaaacccga tttgccagct ataatgattt cgtggtaaaa
    16921 ccggccagag gttctggtgg tgatggaata ctcgtattta aagataaagt ttacggtcga
    16981 tatcgacaaa ttaatggtaa gctgactacc gtgcaggaac tgagttacca tttatcttgc
    17041 ttgttatccg gggcctatag ccttggcgga tcctcggatt atgccatcat tgaaaaacga
    17101 gtggtcgtcg acccggtatt tgcagaggtc agttacgaag gcatacccga tatacgtata
    17161 attaccttat taggctatcc ggcaatggcc atggtaagac ttcccacccg tttatccggc
    17221 ggaaaagcca acttacatca aggagcaata ggcgttggcg ttgatcttgc aacaggaaaa
    17281 actcttggtg gcgtatttca aaatgatacc atagactacc atcccgacac gctaaatccc
    17341 atcgtaggca tcgaagtgcc ctattggaat cagattcttg aaatagcctc cagttgctat
    17401 gagttaactg gtttgggtta tcttggagtt gatattgtac ttgataaaga gcatggtcca
    17461 ttaatgctgg aattaaatgc cagaccagga ttaaatatac aaatagccaa ccgagagggc
    17521 gggcttaagc gctatagaac aattgaagcc cgatttaagg aaaatggcaa agaatctact
    17581 ctggacaaag ttaatttttg ccgcaaagag tttgcacgct aaagcgtcgg tgacgcagaa
    17641 caatttagtt ctttatccac ggataaatta ttccactctt ttaaaaatcg gcgacgagca
    17701 catagccaat cgatcatttc atccatctct acacgctctt ttttagattg aatttttgcc
    17761 agatttcctt gcatattact ccaaaacaat ccttttacat catgtccgtt agcacatcgt
    17821 cttagtgcgg gattcaagca ttcttcatca attttttttg tcagctcttc cctctgtctc
    17881 agcagttgct ctaaaatcaa tttttcagca tggatattct cttgaaatcg cttcaatcca
    17941 actcgatgaa gctcctgctc aaactgatgc tttacggatt caaaagtagt ttctgcaggc
    18001 attcgtccta aaaccatctt tgataaatca agagctggca agcatgcggg gaggcgcaac
    18061 cataatcgtc caaacatgaa ctctccattc aaatcatcaa cgaatttcat tattgccaaa
    18121 caaatacatt tagcaaataa atagactatc aatttttaaa gcacaagttt caatttaatt
    18181 cataatgaaa tataataaaa caacgaatgg atttaattgt taattgcata gaaaatccct
    18241 cctttctgca tatgaaaacg agagagtttt actttgttta aaatccatct ttaatgcaaa
    18301 tcattaacag aacgatacca tactgacgct ctggcatgcc atattcgttg gggagatttt
    18361 ttatgcggtc taaacttgtt tcatttttct cgcgctcgtc aagaccgata aaagctatcc
    18421 cctctaaaac ttggtttgaa acacaattaa aaagcagcgg actcgataaa aaattttcaa
    18481 tagatgagtt gagcgaaacg caatcttccg tcagagtatt tgctaataag aaatatcttc
    18541 cagatactca aacgattaat gaggcattaa ctaaagtaac ggctgtcaat gtgtcaggag
    18601 ataggagcgg gtattttcaa aatggattac cgttcccaaa tgaagcagga tattttgaga
    18661 aaatccctgc aggacatcct gaattattgt ctcccataga aaggttagca agtagtaaga
    18721 aagttgtttc ttcgcacagc ctggtcaccg cgtcaggagg ctatccctta actaatccat
    18781 tattacccta tcgaaaacca attaaagtaa gt
//
LOCUS       DAXLDT010000056        18670 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXLDT010000056 DAXLDT010000000
VERSION     DAXLDT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18670
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.056"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            78..308
                     /locus_tag="ACF2SP_001546"
     CDS             78..308
                     /locus_tag="ACF2SP_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359336.1"
                     /translation="MNKLIVAVTAGASLLLLTGCAVDGVAVDDVSYDSGYSNDYVYSV
                     GYYSNRPYWGNNYYSSPSFVGPYGYWGGDYDF"
     gene            complement(363..932)
                     /gene="efp"
                     /locus_tag="ACF2SP_001547"
     CDS             complement(363..932)
                     /gene="efp"
                     /locus_tag="ACF2SP_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFD2359337.1"
                     /translation="MAVYSTNEFKNGLKVMVDDAPCSILDCEFVKPGKGQAFTRIKIR
                     NLKTGRVVERTFKSGDTLPSADVADVEMQYLYNDGEYWHFMVPDTFEQYAVTENVLAD
                     AAQWLKEQDVCVLTLWNNEPIQVTPPNFVILAITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTGELIKVDTRKGEYVSRAKE"
     gene            1004..1984
                     /gene="epmB"
                     /locus_tag="ACF2SP_001548"
     CDS             1004..1984
                     /gene="epmB"
                     /locus_tag="ACF2SP_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFD2359338.1"
                     /translation="MRDTSLSWQKILAQGFTSTTDLLDFLELPRSEGNLFAEKQFPSR
                     IPLGFAKRMQKGNPKDPLLLQVLAKEDELTEADDYVIDPLSESNTLIKGLLHKYHGRV
                     LLTLTGVCAVNCRYCFRRHFPYQANNPGRRGWKEVCAYIANDPSITEVILSGGDPLLA
                     ANLVLEELLQSLEEISHIHTLRIHTRIPIVLPERIDKGLLDLLTNTRFKKVIVVHCNH
                     PQELDESVLRACSDLKKAACYLLNQSVLLAGINDDAGILSKLSHTLFDYGIMPYYLHL
                     LDKVKGSAHFDMPLPRAQSIYHQLQSLVPGYLLPRLAREEPGRSSKTLLI"
     gene            complement(2053..4125)
                     /gene="ppk1"
                     /locus_tag="ACF2SP_001549"
     CDS             complement(2053..4125)
                     /gene="ppk1"
                     /locus_tag="ACF2SP_001549"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFD2359339.1"
                     /translation="MDTTLDNPDYYINREFSAVAFNQRVLMLANDERVPLLERMRFLS
                     ICSSNLDEFFEIRVAGLKEKIAMSSGKLTIDGLRADEAFSQISHKAHALIDQLYNIFN
                     KQLLPALRKENIHFLETDQWTDDIHLWAKHYFKHEILPVISPIALDLAHPLPRLINKS
                     LNFIISLSGKDAFDRNINYAVVHAPRSIPRMIHLPSELCGDAHYFVYLSSIIKTHINS
                     LFPGMEISGCYSFRLTRNSDLFLREEEIDDLAKAVQREIFSRHYGHVVRLEIENKCPE
                     KIVDFLLQKYHLRHEDTYYCDGPVNLQRYMSAINSIDRPDLNYPAFTPQYPKFSKSQR
                     NLFNVLDEQDILLHHPYQAFDLVIDFVRQAASDPNVLAINQTLYRTHSESVMVDALVN
                     AAHSGKEVTAVIELRARFDEESNLKLANKLHAAGILVLYGVVGYKTHAKMTLVVRRTH
                     GKLKRYVHLSTGNYHEQTAKRYTDLGLLTSEPTITSDAQLIFQQLTGLGKVVKLKALS
                     HSPFTLQKTLLQFIEQCSTAATGGIDTEIILKVNGLTDKVMIQALYKASQAGVKINLL
                     VRGVCCLKPGLPGVSENIRVLSFIGRFLEHHRVFYFRISEEEHYFCSSADLMERNLYN
                     RIEIMFPIYDEGCKKRIKNEIIKNYLKDNNNVWEMQSDGTYKHLMQNGGCAQEKLIAL
                     YEEEDKSI"
     gene            complement(4166..5371)
                     /locus_tag="ACF2SP_001550"
     CDS             complement(4166..5371)
                     /locus_tag="ACF2SP_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2359340.1"
                     /translation="MKRIFFVVFLLCLETACNTAKNNPLPAQLSDKALVPGFKEVRTE
                     FTRSDDPIIKKQSLERVKQLAKAYPDTMFHQKGSFSLLIISGGGDYGAFGAGLLNGWT
                     KSGSRPTFTTVTGISTGALIAPLAFAGPKYDATLKKVYTTITTKDVLSNKPVLWTLLT
                     GGSALKGTKPLQDLIARHVDEKLLDDIAKGYYQGRRLYVGTSNLYSRKLVVWDMTRIA
                     ASKNPERVQLFRKILLASASIPVVMPPVYFDVIANGKPYTEMHVDGSTTFAVFLSRLG
                     SDIKTAKKHFKLKQKPNVKIYVIRNNQNQFHYKVIKPKLLVIGKRAIESVTASQGAAD
                     VIFIYLCALIEGVDFNLANVPNEFDSNSEELFDTQRMNQLYQIGYSKAVKGYPWQKIP
                     PDVRALHGD"
     gene            complement(5447..5980)
                     /locus_tag="ACF2SP_001551"
     CDS             complement(5447..5980)
                     /locus_tag="ACF2SP_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFD2359341.1"
                     /translation="MIKTFIEIIYSFGKIGLISLGGGNSMLKLLEYEAVTYRHWIGKE
                     EFVGMLGSSFLFPGLTGIKLAALIGYKAAGITGLILAIICLNLPGLIMALVGYQWLNS
                     HNSPITRKIMISVQYGALALLAAATFSVVQGVIDVYYSIPMAICCILFFLALAFWNLS
                     PFYGFLAFIAVCFFVVR"
     gene            complement(5977..6597)
                     /locus_tag="ACF2SP_001552"
     CDS             complement(5977..6597)
                     /locus_tag="ACF2SP_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359342.1"
                     /translation="MQRLVWNFEFSSEQTTPLTTLGHDAQDNLKWEKRYFWPADQIIR
                     LNTIDSSLLDLANYQKKHKEDYYYLLPDSDHNIKQRRNELLYKPVIKQSGSVLGFGPK
                     VHLENTQTLSCHGDAGQLNRQELLKKIKEKGMVVFVKKEAFVFKFPTTPTVKLELARL
                     EVKDKVYFSACVEGRSLNLVETISVLLFGPQPSFEYVTFLKKTLKI"
     gene            6773..9211
                     /locus_tag="ACF2SP_001553"
     CDS             6773..9211
                     /locus_tag="ACF2SP_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFD2359343.1"
                     /translation="MAIVFFLIYLAFTLVVLSRRINPLVWEIGSIIYLIPATFYFGFP
                     WIIGLFIWFFIIAAVLVVRVEPLRLFIGDYLYKTAGKSVPKLSKTEEEALNAGDTWLE
                     QDIFTGKPDWQRLSAVSTDLTAEEQAFLDNETHTLCSMLDEWEISQEYDLSPKVWNYI
                     KEKGFLGLVIPKEFGGKGFSARAHSDIVMKIATRSRVAAVTVMVPNSLGPGELINYYG
                     TDEQKAYYLPRLAKGVEIPCFALTEPGAGSDATSIQSEAIVVKKKIDGKSVLGLNITL
                     DKRWITLAPVATLIGLAVNLKDPDGLLKGEGEEGITCLLIPRDTENLEIGNRHLPADQ
                     PFMNGTLRGKDIFVPIDTIIGGQKKAGSGWQMLVECLSIGRSISLPALGAGSSSISYL
                     TTGAFARIRRQFNVEIAQFEGVEEKLAEIAGLNYLINSTRLLTVAAVNEHKKPSVASA
                     ITKYFNTELARITVNASMDVHAGRAIVVGPRNYLNSLYQSVPISITVEGANIMSRNLL
                     IFGQGSMACHPFIRDEFYAISSENKEAFREIIWKHIHYFMQNFAKTICSAWTSGLFIA
                     VPENSMKREYKKLARLSYAYSWLADLSLIYLGGDLKRKERLSARLADGMSYLYMAMAA
                     LRNVQLNQDHPDEQLHARWAVSYCFYHAQKSMIALCHNFPSKFLGFIARVVAFPFGQT
                     MRYPSDKLDHQLARLMTGNNHYRDRLKKSVFLSGDSKQPVDRMEHALQLIIEADGLSK
                     KVSDLKRYKWSKLKEKLKEKVESGQLSQQEMEELLAVEDARWDAILVDEFTFDSMKKK
                     TLHSVIEKMKSPLA"
     gene            9325..10017
                     /locus_tag="ACF2SP_001554"
     CDS             9325..10017
                     /locus_tag="ACF2SP_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0294 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359344.1"
                     /translation="MPKYTLPTRDALLKAMQVGETSIEAAEYMATRFEQILTKAKLLP
                     ECNDMLEKIKEYAQFVKFKLLSSAQVWSGQERPTSDYQNTQENKAEFLASHLEGLPSG
                     LKLEVAIGDDAKILRGFSSNGKMVEGDQLKTMDGLLEGWLAKNGLAISGGAVVKIDNT
                     GNQIKVDPQEIRQLINDSEKGVAKYFADKGVSMEVAQRTYQEPKALETKREEIRQEIE
                     SGAEAPTTQSIR"
     gene            complement(10054..10716)
                     /gene="murU"
                     /locus_tag="ACF2SP_001555"
     CDS             complement(10054..10716)
                     /gene="murU"
                     /locus_tag="ACF2SP_001555"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFD2359345.1"
                     /translation="MKTAMILAAGRGERLRPLTDKIPKALCTVRNKPLIEHHIINLAN
                     AGFERLIINHAYLGGQIRQYIGNGKQWGLNVIYSPEPPGGLETGGGIVNALPLLGEEP
                     FLTVNADIYTDFDFAKLRLKNIDTFHLLLIPKNPSLLHHGDFGLINGSHLTNTNQAYT
                     FSGIACYNPKVFANCRQGRYSVTPLLRKYVEQNSATGSVHSGIWYDIGSFDRLQAANQ
                     LT"
     gene            complement(10713..11690)
                     /locus_tag="ACF2SP_001556"
     CDS             complement(10713..11690)
                     /locus_tag="ACF2SP_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2359346.1"
                     /translation="MHERENALKEWLAQTIQEKDFVLLPLAGDASFRRYFRIQYNGLT
                     QVVMDAPPEKENIEPFLHIANVLDKVKIPVPDILAINKREGFLLLSDLGDQLLLNKLN
                     HETVDNYYSQAMNLLLQIQKCPTGDPELPLFDKGFMLKEMNLCLEWFLKAYLSLDLKQ
                     DELQLFQHTIEWIASEVATQPRVFIHRDYHSRNLMLVKNKDSCLATIDFQDAMLGPVT
                     YDLVSLLKDCYISWPREKVLEWITYFHEKCPHANIYSLTDFIRAFDLCGLQRHLKVLG
                     VFCRLYLRDNKAGYLRDLPLTLKYALECAETYEELHPFFNFLQKRVYLP"
     gene            11933..14452
                     /locus_tag="ACF2SP_001557"
     CDS             11933..14452
                     /locus_tag="ACF2SP_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFD2359347.1"
                     /translation="MAIGITACVLSLINYQGLAYSAALINEPIQACVIAREVDLTDDI
                     RTKFAQCLGWQADQSSPVCLGFYKPITVTPLASPDEVRILADTASFYRTQRSTLSGHV
                     EMQQGQRVVNAQTAYVYRDPKTNEVTKIEFLNHVRYLEPDRMMIARKAVVYPQDKSGE
                     VEDVLYRFNTNRSNALLPAWGRASLIKRFANQDYFLKEATYTTCAPQDKAWAIEAESI
                     SIDNEKGKGIARNAKLRIHEWPVLYTPYLSFPTNRDRKSGFLMPIVGYSNVGGADLGI
                     PYYWNMAPNYDMTLVPHLYTKRGLMLGGQFRYLTSKSTGTFNGNFLPKDKAFGRFLQD
                     NEVEFPQIRGLSTNRWEVNFVDSTQFLSDLQLNVNFQQVSDDYYLQDFSTNLASVTQR
                     QLLRQADLTYTTENWTFRGMGQSYQTLHPINEIPVSPVYERLPQLMARGYYDDLPFNA
                     NFNILGQYDQFHWPNDSWNIALNNMPQGPRFHLNPILSVPMMKPWGYVTPSVQFVENY
                     YDISRNYTWGTSRANYNLTIPRYSLDGGLYFERDLHLKGTYYIQTLEPRLFYLRVPYY
                     NQTLIPVYDSGFMIFNVDQLFRTNRFSGFDRIGDANQLSYALTTRWLEDESGAEKANF
                     SIGQIKYFSERRVQLCQSPTGFCTDNPDTFGNLSSTFGTSPVASRAVYKFNPAWGITG
                     DYIWDPATRATNNADLNLHYQPARNAIINGGYSYLVNGDVTQVRNNDTENNALHQAIL
                     SAAWPLSEKWSGIGAYSYNISKNYSMMSFLGVQYDSCCWAMRILGGRTFRSLNEEFEP
                     RYNNNIYLQILLKGLGSVASSDPSGILNTYIPGYYDPFRRR"
     gene            14628..15917
                     /locus_tag="ACF2SP_001558"
     CDS             14628..15917
                     /locus_tag="ACF2SP_001558"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD2359348.1"
                     /translation="MFKRIALVCALFSGICFAEGKQLLDKVVAIVNDNVITSSELNAQ
                     VELSKKQIIAQNMQMPDESVLRKQVLQHLIDVDLEMQMAKQNGITIENAEIDEAIEKI
                     AASNHLNLSQMRDEITKQGISWQEYRQNIRKEMLISRVQQKAVGKDIIVTNEQVEQYL
                     KNAGRIENSNLTYHLKNIVIPLSEEPTTKQLQRAKIEAENLLNKIKKGEDFSRLAIEE
                     SSGEFALEGGDLGERHLAELPEVFAKEVVHMKVGQVAGPIRAGNGFHLIKLVAVGGEN
                     QRHVITQTHVRHILLKPDASMVPSEAIKQVNNIYRQIQSGKDFALMAKQYSLDAASAV
                     KGGDLGWVNPGELVPEFEKTMNSLPLHKVSKPVKTQYGWHLIEVIARRQKDDSEAFKK
                     QQVRQFLQQRKFVEAVQNWQQHLRSQAYINIVDKDLA"
     gene            15914..16888
                     /gene="pdxA"
                     /locus_tag="ACF2SP_001559"
     CDS             15914..16888
                     /gene="pdxA"
                     /locus_tag="ACF2SP_001559"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFD2359349.1"
                     /translation="MNPLLISSGEPAGIGPDLCLALAETNLPVVILGDLSLLEARASE
                     LNLSIKFLEYGPHQSFEKKAGYLTVWPVPCAAPVISGELNPQNAAYVIELLTLGASLC
                     SKGEFSALVTAPVHKANINAAGITFTGHTEFFADFFEVETVVMMLACSHMKVALVTTH
                     LPLRMVPDAINSLLIIKVIQQLHHSLKHDFGIQSPKINVAGLNPHAGESGYLGREEIE
                     IITPALNTLKNQGIDVLGPLPADTMFITNNINHCDAYVAMYHDQGLPVLKYAGFNEAV
                     NITLGLPIIRTSVDHGTALELAGKNKANPGSMLAAVKMAKDMALTRIK"
     gene            16890..17381
                     /locus_tag="ACF2SP_001560"
     CDS             16890..17381
                     /locus_tag="ACF2SP_001560"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFD2359350.1"
                     /translation="MSLISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIM
                     GRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSLGQAIKETADASEVMIIGGAKIY
                     SQAISIANRLYITRIHHQFMADVFFPKINELVWSCRSKEYRQHDNKNQYDMTFCIYER
                     ISI"
     gene            complement(17388..17933)
                     /locus_tag="ACF2SP_001561"
     CDS             complement(17388..17933)
                     /locus_tag="ACF2SP_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HFD2359351.1"
                     /translation="MSKLVYLLLMIFFLNPAWANTHYHQHPVSPLANALRTGTDTNGN
                     ALYLCKAKLFNSIQPGKTWAGYGRCNVPYGGKEYVLSQFTIPNQNEFGRYSWEPNVEH
                     ALLMGKDTNGNPLFVCQSNFNGSIQPGKTWPGYSHCNISYGGREIITDNYRILSRPTE
                     IIVRTQPPVHRHPSQNRHYHY"
     gene            18221..>18670
                     /locus_tag="ACF2SP_001562"
     rRNA            18221..>18670
                     /locus_tag="ACF2SP_001562"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5680 a         3425 c         3903 g         5662 t
ORIGIN      
        1 ttaaaataaa gaacaatttg agcaattaat aatactatct tataattaaa gaacaactat
       61 tttgccagga agcaattatg aacaagttaa ttgttgcggt cactgctggt gcatcattat
      121 tgctgctaac tggatgtgcg gttgatggtg tggctgttga tgatgtttct tacgattcag
      181 gttatagcaa tgactacgtt tattcggttg gatattatag caaccgccct tattggggta
      241 acaactacta ttcgtcacct tcttttgtag gtccttatgg atattgggga ggagattacg
      301 atttctaatt tccggggaga aggcaagcct tcaaatcaaa tgaaggctat ttctgttgat
      361 aattactctt tagcgcgaga cacatattcg cctttgcgcg tgtctacctt aatcagttcg
      421 ccagtttgaa caaacaatgg tacacgaaca acggcacctg tttccaatgt agctggcttt
      481 ccgcctccac cagaagtgtc tccttttaat ccaggatctg tttcggtgat tgccaaaatg
      541 acaaaattgg gtggcgtgac ctgtataggt tcattattcc atagagtaag tacacaaacg
      601 tcctgttcct ttaaccattg tgctgcatcg gctaatacgt tttcagttac ggcatattgt
      661 tcaaacgtat ctggaaccat aaaatgccaa tactccccat cattatatag atactgcatt
      721 tctacgtcag ccacatcagc agatggtaag gtgtccccag atttaaaggt acgttccaca
      781 acgcgcccgg tttttagatt gcgaatttta atacgggtga acgcttgacc ttttccaggt
      841 tttacaaatt cacagtcaag aatgctgcac ggagcatcat caaccattac ttttaagccg
      901 tttttaaatt catttgtgct atagactgcc atttctgctc catagttgag attttgctgt
      961 attttcgcta aagttcgcac agtttatcaa gtctgtaact ttaatgcgag atacctcttt
     1021 gagttggcaa aaaattttgg cgcaaggatt tacttcaacg actgatttat tagatttcct
     1081 ggaattgccg agatctgaag gaaacctatt cgcggaaaaa caattcccaa gccgtattcc
     1141 tttaggtttt gctaagcgaa tgcaaaaagg gaatcccaaa gatcccttgc ttttacaggt
     1201 tcttgcaaaa gaagatgagc ttaccgaagc agatgattat gttatcgatc ctctgagtga
     1261 gagtaatacc cttattaaag gtttgttgca taaatatcac gggcgtgttt tgttaacttt
     1321 aaccggtgtt tgcgctgtga attgtcgtta ttgctttaga agacattttc cttatcaagc
     1381 gaataatccg ggccgccggg gatggaaaga ggtgtgtgcc tacatcgcaa atgatcccag
     1441 tattactgaa gttattttaa gtggtggcga tccattgctc gctgccaatt tggttttgga
     1501 agaattactc caatctttgg aagaaatttc tcatatacat accttaagaa ttcatacaag
     1561 aattcctatt gttttaccgg agcgtattga caaaggatta cttgatttat taacaaatac
     1621 gcgatttaag aaagtgattg ttgttcattg taatcatccg caagagttgg atgagagtgt
     1681 attgcgggcg tgctctgatt tgaagaaagc agcatgttat ttactgaatc aatctgtttt
     1741 attagccggg attaatgatg atgcagggat tttatccaag ctaagtcata ctctttttga
     1801 ttacgggatc atgccttact acttgcattt gctcgataaa gtaaagggtt cggcacattt
     1861 cgatatgccg cttcccaggg ctcagtcaat ttaccatcaa ttacaatccc ttgtgccagg
     1921 atatttattg cctcgcctgg cacgagaaga gccgggaaga tccagtaaaa ccttgctgat
     1981 ttaaagagct tgatatgtcc tggctattta tggttttatt caatagccat gttttcactg
     2041 ttatttttct gactaaatcg atttgtcctc ttcctcataa agtgcaatca gcttttcctg
     2101 agcacagccg ccgttttgca tgaggtgctt gtaagtcccg tcactttgca tttcccaaac
     2161 attattatta tctttaagat agtttttaat gatttcgttt tttattcttt ttttacatcc
     2221 ctcatcataa atggggaaca tgatttcaat acgattgtat aaatttcttt ccatcagatc
     2281 ggcactggag caaaaatagt gttcttcttc gctaatgcga aaataaaaca ctctgtgatg
     2341 ttcgaggaat ctgccaataa aagacaagac tctgatgttc tcagacacgc caggcaatcc
     2401 tggttttaaa caacacaccc cacgtacaag aagattaatt ttaacgcctg cctgtgatgc
     2461 cttatataat gcttgaatca tcactttatc agttaatcca ttcactttca ggatgatttc
     2521 agtatcaatg cctcccgtag cagcagtact gcattgttcg atgaattgta aaagagtttt
     2581 ttgtaaggta aatggcgaat ggctcaaggc ttttaactta acaacctttc caagaccggt
     2641 taattgttga aaaatgagct gggcgtcaga agtaatagtg ggttcactgg ttaataatcc
     2701 caaatcagtg tatctcttgg ctgtttgttc atgatagttt cctgtgctta agtgcacata
     2761 tcgttttaat ttaccatgcg ttcttcgaac taccagcgtc attttcgcat gagttttata
     2821 gcctactact ccgtagagta ccaggatccc cgcagcatgg agtttgttag ccaattttaa
     2881 gttggattcc tcgtcaaaac gggctcttag ctcaatgaca gcggttactt ctttccctga
     2941 atgggcggcg ttaaccagcg catcgaccat cactgattca gagtgggttc tgtaaagcgt
     3001 ttgattaatt gccagtacat tcgggtctga agcagcttgc ctgacaaaat caatgactaa
     3061 atcaaatgct tgatagggat gatgtaataa aatgtcttgt tcgtcgagaa cattaaataa
     3121 attacgttgt gatttggaga acttgggata ctgtggggta aaagcgggat aattcagatc
     3181 aggtctatcg atgctgttaa tagcactcat atatcgttgt aaattaacgg gcccatcaca
     3241 ataataggtg tcttcatgtc gcaaatgata tttctgcagg aggaagtcaa caattttttc
     3301 agggcatttg ttttcaattt ccaatctcac gacatggcca taatgacggg aaaatatctc
     3361 tctttgcacc gctttagcca aatcgtcaat ttcttcttct cttaaaaata aatcactatt
     3421 tcgtgtcaac cgaaatgaat aacatccgct tatttccata cctggaaata agctattaat
     3481 atgcgtttta ataatggaag ataaataaac aaagtaatgg gcatctccac aaagctcaga
     3541 aggcaaatgt atcatgcgtg gaatagagcg gggagcatgt actacggcgt aattgatatt
     3601 tcgatcaaaa gcgtctttcc cactcaatga aatgataaaa tttaaacttt tattgataag
     3661 ccttggaaga ggatgggcca aatccagagc aatagggctt ataacaggta gaatttcgtg
     3721 tttaaagtaa tgctttgccc ataaatgaat gtcatcagtc cattgatctg tctcaagaaa
     3781 atggatattt tctttgcgca atgccggaag taattgtttg ttaaaaatat tgtaaagctg
     3841 atcgattaaa gcatgagctt tatggcttat ttggctgaat gcttcatcag cccgtaatcc
     3901 atctatggtt agcttgcctg acgacatggc aattttttct ttcaacccgg ccacccgaat
     3961 ttcaaaaaat tcatcaagat tactgctgca gatactgaga aatctcattc tttccaataa
     4021 gggtacgcgt tcatcattag caagcatgag tactctctga ttaaacgcaa cagcagaaaa
     4081 ttctctatta atataataat ctgggttatc cagagtagta tccatgagga actccaggta
     4141 ttttcatttc atgttcacag gcaatttagt caccatgcaa agcccttaca tctggaggga
     4201 ttttttgcca ggggtaacct tttaccgctt tggaataacc aatttgatat aactgattca
     4261 ttctttgagt atcaaacaat tcctccgagt tcgaatcaaa ctcatttgga acattggcta
     4321 aattaaaatc aactccttct ataagagcac aaagatagat aaaaataaca tcagcagcac
     4381 cctgggatgc cgtgacactt tctatcgctc gcttgccaat taccaataac ttgggcttga
     4441 ttactttgta atggaattga ttctggttat tgcgtattac ataaatttta acgtttggtt
     4501 tttgttttaa tttgaaatgt tttttcgcgg ttttgatatc agaaccaaga cgacttaaaa
     4561 agaccgcaaa cgttgttgag ccatcgacgt gcatttctgt gtagggtttt ccgttggcta
     4621 tgacatcaaa ataaacaggc ggcatgacca cgggaatgct ggcagaagca agtagaattt
     4681 ttcggaataa ctgaactctc tcggggtttt tgcttgccgc aatgcgggtc atatcccata
     4741 ccaccagctt tcgagagtaa agattggatg tccctacata aagccgtcgt ccttgataat
     4801 aacctttggc aatgtcatca agcaattttt catcgacatg acgagcaatc aggtcttgta
     4861 atggtttggt tcctttaagg gcgctgcctc ctgttaacag agtccataat acaggtttat
     4921 tactcagtac atccttcgtg gtaatggttg tatacacttt ttttaatgta gcatcatact
     4981 tggggccagc aaaagctaaa ggtgctatca atgccccagt acttatacct gtcacggttg
     5041 taaatgttgg ccgagaaccg gattttgtcc acccattcag taatccagca ccaaaagcac
     5101 cataatcgcc accacctgaa ataatcagca agctgaaaga gcctttttga tggaacatgg
     5161 tatcagggta agctttagcc agttgtttta cacgttcaag agattgcttt ttaattattg
     5221 ggtcatcact gcgggtgaat tcagtgcgta cctctttaaa tccaggcact aaggccttat
     5281 ctgataattg cgctgggaga ggattatttt ttgctgtatt gcaagcggtt tctaaacaaa
     5341 gcaaaaatac aacaaaaaaa atccttttca taattttcct ttaaattaat gttttcctga
     5401 attttattca aaaaatggat taaaagcgat agtgtttgtt ttcatattaa cgcaccacaa
     5461 aaaaacatac ggctataaat gctaaaaagc cataaaaggg tgatagattc caaaaggcca
     5521 aggcaagaaa aaacaaaata cagcaaatag ccataggaat agagtaatag acgtcaataa
     5581 caccttgaac tactgaaaat gtagcagccg ctaacaaggc caaagctccg tattggactg
     5641 agatcataat tttgcgtgtt attggactgt tatgagaatt aagccactga tatcccacaa
     5701 gagccatgat taatcctggt aaattcagac agatgatcgc aagaattaaa ccggttattc
     5761 cggccgcttt atagcctatt agtgcggcca gttttattcc cgttaatcct gggaataaaa
     5821 aacttgaacc taacatacct acaaattctt cttttcctat ccagtgcctg tacgtgactg
     5881 cctcatattc caataatttt aacatggaat tgccgccgcc tagggatatc aggccaattt
     5941 tcccaaaact gtaaatgatt tcaatgaaag tttttatcat atttttaaag tcttttttaa
     6001 gaaggtaacg tattcaaaag aaggttgggg tccgaataaa aggacgctga tggtttctac
     6061 caggtttaat gatctcccct caacacaggc actgaaatac actttatcct tgacttcaag
     6121 acgagcaagt tcaagcttga cagttggcgt tgtagggaat ttaaaaacaa aggcttcttt
     6181 tttaacaaaa acaaccattc ctttttcttt tatttttttc aatagttctt gtcgattcaa
     6241 ttgcccagcg tcgccatggc aagagagagt ttgtgtgttt tcaagatgaa ctttaggccc
     6301 aaaacctagt acagagccag attgcttaat aacaggttta tacaataatt cgttacgcct
     6361 ttgttttata ttatgatcag agtcaggcag taaataataa tagtcttctt tatgtttttt
     6421 ctgatagttg gctagatcaa gcaacgaact gtcaatagta tttaaacgga tgatttgatc
     6481 tgcaggccaa aaatagcgct tttcccattt tagattgtct tgagcatcat gacctaaagt
     6541 ggtaagtggt gttgtttgtt cagaggaaaa ttcaaagttc caaaccagtc tttgcattat
     6601 tagtatttca ttttctgttt tgcttacgct agatgatttg atttgttttg gcaaggttaa
     6661 cttcaatgtt tcttataatt tttaaaggat tattgctcgc taaataaagc gtaaaaagta
     6721 tatactggtt attctataaa cctcttaata aaatagcaaa ggaaggtatt ttatggctat
     6781 tgtgttcttt ttaatttatt tggcttttac tctggtagta ctgtccagaa gaattaatcc
     6841 attggtttgg gaaattggaa gcataatcta cctcattccc gcaacatttt attttgggtt
     6901 cccctggatt atcggtttat tcatttggtt ctttattata gctgccgtgc tggtagttcg
     6961 agtggagcca ttacgccttt tcatcgggga ttatttgtat aaaactgcag gaaaatcggt
     7021 acccaagctc tctaaaacgg aagaagaagc tttaaacgcg ggggatacct ggctggaaca
     7081 agatattttt acaggaaaac ctgattggca aagattatca gcagtttcta cagatctcac
     7141 agcagaagaa caagcttttt tagataatga aactcatact ttgtgcagta tgttggatga
     7201 atgggaaatt agccaggaat atgatttatc tcccaaggtt tggaattaca ttaaagagaa
     7261 aggattttta ggattagtga taccaaagga attcggtggt aaagggttct cagctcgagc
     7321 ccattccgat attgtaatga aaattgcaac tcgctcaaga gtagcggcag tgacggtaat
     7381 ggttcctaat tccctgggcc cgggtgagtt aataaattat tatggaactg atgagcaaaa
     7441 ggcatattat cttccgcgtc ttgccaaagg tgttgagatc ccctgttttg cattgacaga
     7501 gccaggggct ggaagtgatg cgacctcaat ccagtcagaa gccattgtag ttaaaaagaa
     7561 gatagatgga aaatcagtac ttggcttaaa tatcaccctg gataaacggt ggattacttt
     7621 agctcctgtt gcgactttaa ttggattggc cgtgaacttg aaagatccgg atgggctttt
     7681 gaagggtgaa ggcgaggaag gcataacttg cttgctgatt ccaagagata ctgaaaatct
     7741 tgaaattggc aataggcatt taccagctga tcagcccttt atgaatggaa ctttacgtgg
     7801 caaagatatt tttgtgccta ttgataccat cattggtggt caaaagaaag cgggatccgg
     7861 ttggcaaatg ctggttgagt gcttatctat agggcgctca atatctctgc cagcacttgg
     7921 agcaggttct tcttctattt cttatttaac aacaggtgct tttgctagaa ttcgtcgcca
     7981 atttaatgta gaaatcgctc agtttgaagg agtagaggaa aaacttgcag aaatagcggg
     8041 tttgaattat ttgatcaact ctacccggtt gttgacggtt gctgctgtga atgagcataa
     8101 gaaaccctct gtagcttcag cgattaccaa atattttaat acggaattgg cacgaatcac
     8161 tgtgaatgct tctatggatg ttcatgctgg acgagccatt gttgttgggc cacgcaatta
     8221 cttaaatagt ttatatcaaa gtgttcctat ttctattacg gtagaagggg ctaatattat
     8281 gtctcgcaat ttattgattt ttgggcaggg atctatggcc tgtcatccgt ttattcgtga
     8341 tgaattctac gccatctcca gtgagaataa agaggcattt agagagataa tttggaagca
     8401 tatccattat tttatgcaga attttgctaa aacaatctgt tcagcatgga caagtggttt
     8461 gtttattgcg gttcccgaaa attcaatgaa aagagagtat aagaaactgg cgcgtttgag
     8521 ttatgcctat tcctggcttg ctgatctgtc tttaatttat ttgggaggtg atttaaagcg
     8581 caaggagcga ttgtctgcaa gattagccga tggcatgtct tatctctata tggcaatggc
     8641 tgctttacga aatgtgcaat taaatcaaga ccatcccgat gagcaattgc atgccagatg
     8701 ggcagtaagc tattgttttt accacgcgca aaaatccatg attgcacttt gccacaattt
     8761 cccttccaaa tttttaggtt ttattgcccg agtagttgcg tttccatttg ggcaaaccat
     8821 gcgttatcct tcagacaaac tagatcatca gttggctcgt ttgatgacag gcaacaatca
     8881 ttatcgtgac cgtttgaaaa aatcggtctt tctcagtggt gattccaagc agcctgtgga
     8941 tcgtatggaa catgctttac agcttattat tgaagcggat ggattaagca aaaaagtgag
     9001 tgatttgaag cgctataagt ggagtaagtt gaaagagaag ttaaaagaaa aagtagagtc
     9061 tggccagttg tcacaacagg aaatggaaga gttacttgct gttgaagacg cacgttggga
     9121 tgccatttta gtggatgaat ttaccttcga ctccatgaag aagaagacat tgcattccgt
     9181 gatagaaaaa atgaaatctc ccttggcgtg atttttacag acttttaaca tcgatttgcc
     9241 aaccagctat caatccaaat gaggctggtt tgctataatt aaactatatt tcctggacca
     9301 aatatacatt gttgagagta agttatgcca aaatacacac tgcccacaag agatgcctta
     9361 ttaaaagcaa tgcaagtagg tgaaacatcc attgaagccg ctgaatatat ggcaacaagg
     9421 tttgagcaga ttttaacaaa agctaagtta ttaccagaat gtaatgatat gctggaaaaa
     9481 ataaaagaat atgctcaatt cgttaaattc aaattattat cctctgctca agtttggtct
     9541 ggccaagaaa ggccaacttc agattaccaa aacacgcagg aaaacaaagc cgaatttttg
     9601 gcaagtcatt tggaagggtt gccaagtgga ttaaaactcg aagttgctat tggggacgat
     9661 gccaaaatat tgcgaggatt ttcttccaat ggaaaaatgg tagaaggaga tcagctcaaa
     9721 acgatggatg gattattaga aggatggctt gccaagaatg gtcttgctat ttcaggaggt
     9781 gcggttgtta aaatagacaa cacgggcaac caaatcaaag tagatccaca ggaaatcaga
     9841 caattaatca acgatagcga aaagggagtc gcgaaatatt ttgcagataa aggcgtgagt
     9901 atggaagtgg cacaacgaac ctaccaagaa cccaaagcat tggaaaccaa gcgagaagaa
     9961 atcaggcaag aaattgaatc cggtgctgaa gctccaacca ctcaatccat cagataggaa
    10021 tatactggct cacctaaaaa ccaagattgg tttttaggtg agctgatttg ctgcttgcag
    10081 tctatcgaaa gagcctatgt cataccaaat tcctgagtga acacttccag tggcactatt
    10141 ttgttccaca tattttctga gtaaaggggt aacagaatac cttccttgtc tgcagttggc
    10201 aaaaaccttt gggttatagc aggcaatacc tgaaaaagtg taagcctgat ttgtattggt
    10261 taagtgggat ccattgatta aaccaaaatc gccatgatga agcaaagaag gatttttggg
    10321 aattaaaagc agatgaaatg tatcaatatt tttgagtcgg agcttggcaa aatcaaaatc
    10381 agtataaatg tcggcattta ctgttaaaaa tggctcctcg cccagcaaag gcaaggcgtt
    10441 cacaatccct cctcctgttt caaggcctcc tggcggttct ggagaataga taacatttaa
    10501 gccccattgt tttccatttc ctatatattg gcgaatttgt ccgcccaggt aggcatgatt
    10561 aattatcaat cgttcaaagc cggcattggc caggttaatg atatggtgtt caattaaagg
    10621 tttgtttctt accgtacaaa gtgctttagg aattttgtcg gttaaagggc gtaatcgttc
    10681 tccacgtcct gctgcaagaa tcatagcggt tttcatggta aataaactcg tttttgcaga
    10741 aaattaaaaa atgggtgtaa ttcttcatat gtttctgcgc attctaacgc atattttaac
    10801 gtgagaggca agtccctaag atatcctgct ttattatcac gcaaatataa acggcaaaaa
    10861 acacctaata cttttaaatg cctttgtaac ccacatagat caaaggcccg aataaaatca
    10921 gttagagaat aaatattggc gtgggggcat ttttcatgaa aataggttat ccattctaaa
    10981 accttttctc tcggccatga aatataacaa tctttgagta aagaaactaa atcataggta
    11041 acaggaccta gcatagcatc ctgaaaatca atagttgcta aacagctatc cttatttttg
    11101 actagcatca gatttcttga atgatagtcc ctgtgaatga aaactcttgg ttgagtcgct
    11161 acttcacttg cgatccactc gatggtatgc tgaaataatt gtaattcgtc ttgttttaaa
    11221 tcaagagata gataggcttt caaaaaccat tccaaacata aattcatttc tttgagcatg
    11281 aaacccttat caaataaggg aagttcagga tcgccagtgg gacatttttg aatttgtaat
    11341 agcaaattca ttgcctggct ataataatta tccactgttt catgattcag cttatttaat
    11401 agtaattgat cccctaaatc gcttagtaat aggaaacctt ctcttttatt tattgccaga
    11461 atatcgggga ctggtatttt aactttatca agaacgttag cgatatggag gaaaggttca
    11521 atattttcct tttcaggtgg agcgtccata actacttgag ttaatccatt gtattgaatg
    11581 cgaaaatacc ttctaaaact tgcatctcct gctaaaggaa ggaggacaaa gtctttttct
    11641 tgaatcgttt gagctaacca ctctttcagt gcgttttctc tttcatgcat ggtatacttc
    11701 ccgtttttat ttacagagtg cggttgatat actcccttct taataaagag taatttcatt
    11761 cgcgcacaaa taataaggta ttttgttagc tgtgaacatg aggttttata aaaagctaat
    11821 tatatccggt tttatttcat ttatcgcctt attgcgaggt ttactctatt gccatttaaa
    11881 aaggccattt aaaaaatggc ctgaatcttt attttcagtg aaatgggttt taatggccat
    11941 agggataact gcttgtgttt tatctttgat aaattatcaa ggcttagcct attctgctgc
    12001 cttgataaat gaacctattc aagcttgcgt gattgcgcga gaagtcgatt taacagacga
    12061 tataagaact aaatttgccc aatgtttggg ttggcaagct gatcaaagtt cccctgtgtg
    12121 cctcggtttt tacaaaccca ttacggtgac accgttagct agtccagatg aagtacgtat
    12181 tctggcagat acggcttcct tctatcgcac tcaaagatcc actttatctg ggcatgtaga
    12241 aatgcagcag ggccaaagag tagttaacgc tcaaacagcc tatgtgtaca gggatcctaa
    12301 aacgaatgaa gtaacaaaaa ttgaattttt gaatcatgta cgttatttag agcctgatag
    12361 aatgatgatc gctagaaaag cagttgtgta tcctcaagat aaatcaggcg aagtggaaga
    12421 tgttctttat cgatttaata ccaatagaag caatgcattg ctgcctgcct ggggtagggc
    12481 aagtttaatc aagcgttttg caaatcagga ctactttttg aaagaggcaa cttacacgac
    12541 ttgcgcgcct caagataagg cttgggctat cgaggccgaa tccatcagta tagacaacga
    12601 aaaaggaaag ggcattgctc gcaatgccaa attacgtatc cacgaatggc cggtgcttta
    12661 taccccttat ttaagttttc ctaccaatag agatagaaaa tcggggtttc tgatgcccat
    12721 agtgggttat tccaatgtag ggggagctga tttgggcatc ccttattatt ggaatatggc
    12781 gccaaattat gacatgactc tcgttcctca cctttataca aaacgcggct tgatgttggg
    12841 aggacaattt agatatttga cttcaaagag cactggaaca tttaatggaa attttttacc
    12901 aaaagataaa gcctttggca ggtttttaca ggacaatgag gtagagttcc ctcagattcg
    12961 aggactttcc acgaatcgtt gggaagtaaa ttttgtcgat tcaacccaat ttctctctga
    13021 tcttcaatta aatgttaatt ttcaacaggt ttctgatgat tattacttgc aagatttcag
    13081 tacaaatttg gcatcggtaa ctcaaagaca attattgcgc caggctgatt taacctacac
    13141 taccgaaaac tggactttta gaggtatggg acaaagttat caaacgttac atccaattaa
    13201 cgaaatcccg gtttcccctg tttatgaacg gttaccgcaa cttatggcac gaggttatta
    13261 tgatgattta ccttttaatg ccaatttcaa catccttggt caatacgacc agtttcactg
    13321 gcctaatgac tcctggaata tagcacttaa taatatgcct caggggcccc ggtttcatct
    13381 gaatcccatt ttatcagtgc caatgatgaa gccttgggga tatgttactc cctcagtgca
    13441 atttgtggaa aattattacg atatttcaag aaattatacg tggggcacat ctcgagctaa
    13501 ctataacctg acaatacctc gctatagcct tgatggcgga ttgtatttcg aacgagattt
    13561 gcatcttaag ggtacgtatt acatacaaac tttagagccc agattattct atttaagagt
    13621 accttattat aaccaaacac ttattccagt ttatgattca ggatttatga tttttaatgt
    13681 cgatcaattg tttagaacca atcgcttttc agggtttgat agaattggag atgccaatca
    13741 gctaagttat gcccttacca caaggtggtt agaagatgaa tcaggtgctg agaaagcgaa
    13801 tttttctatt ggtcaaatca aatatttcag tgagagaaga gtacaacttt gtcaaagccc
    13861 taccgggttt tgtaccgata atccagatac ctttggaaac ttatcttcta cttttggaac
    13921 atcgcctgtt gcgtcaaggg cggtatataa atttaatcca gcttggggaa taacagggga
    13981 ttacatttgg gatccggcaa caagggctac aaataatgca gatttgaatt tgcactatca
    14041 gccagctcgt aatgctatca tcaatggcgg gtatagttat ttagttaatg gtgacgtcac
    14101 tcaagtaaga aataatgaca ctgaaaataa tgccttacat caagctattt tatccgctgc
    14161 ctggccgctt agtgaaaaat ggagtggtat tggagcctat agttataata tcagtaaaaa
    14221 ttacagtatg atgtcatttc ttggcgttca atatgatagt tgttgttggg ctatgcgtat
    14281 tttgggaggg cgaactttta gaagtttaaa tgaagaattt gagcctcggt acaataacaa
    14341 catttatttg caaatattac taaaaggctt aggatcagta gctagcagtg atccaagtgg
    14401 tatattgaat acctatattc caggatacta tgaccctttc cgacgccgtt aaaatccaaa
    14461 gataaatggc gcaataaaag cgagtcaggt ttagatgttt aggttagtac aggttgaaaa
    14521 tattttaatc tgttttttgt gaattatggt gtaaattaag caaacttgct tgtattttga
    14581 caaagaataa cataaattgc ccgaaaaatg taaatgagga ttatggcatg tttaaaagaa
    14641 tagcattggt atgcgctttg ttttcaggaa tatgtttcgc tgaaggaaag cagctgttag
    14701 ataaagtggt tgctatagta aatgataatg tcattacttc cagcgaattg aacgctcaag
    14761 tcgagttatc taagaagcag attatcgctc aaaatatgca aatgccagat gagtctgtat
    14821 tacgtaagca agtattgcaa catttaatag acgtagatct ggaaatgcaa atggcaaaac
    14881 aaaatggtat tactattgaa aatgctgaaa ttgatgaagc gatagaaaaa atagctgctt
    14941 caaatcatct aaatttatcg caaatgcgcg atgaaattac aaagcaagga attagctggc
    15001 aagaatacag acaaaatatc cgtaaagaaa tgctgatttc cagggttcag caaaaagcag
    15061 taggtaaaga tatcattgtt actaatgagc aggtggaaca atacttaaaa aatgcaggcc
    15121 gcatagaaaa ttctaacttg acgtatcatt taaaaaatat tgttatccct ttaagcgaag
    15181 aaccaacgac caaacagctt caaagagcta aaatcgaggc agaaaacttg ttaaataaaa
    15241 ttaaaaaagg tgaagacttt agccgtttgg caatagaaga atcaagtgga gagtttgcac
    15301 tggaaggtgg tgatttgggt gagcgtcatt tggctgagct gcctgaggta tttgccaagg
    15361 aagtagtaca tatgaaagta ggtcaggtgg ctggcccgat aagagcagga aatggatttc
    15421 atttgatcaa attagtagct gttggcggcg agaatcagcg tcatgtgatt actcaaaccc
    15481 atgtcagaca tatactgctt aagcccgatg ccagcatggt gccttctgaa gcaattaagc
    15541 aagtcaataa catttaccgg caaatacagt caggcaaaga ttttgctctt atggctaaac
    15601 aatattcact cgatgctgcc agcgcagtga aaggcggtga tttaggatgg gttaatcctg
    15661 gtgaattagt tccagagttt gaaaaaacca tgaacagttt gccattacat aaagtcagta
    15721 aaccggtaaa aacccagtac gggtggcatc tcattgaagt gattgcacgc agacagaagg
    15781 atgattcaga agcttttaag aagcaacagg tcaggcaatt tctacaacag cgtaagtttg
    15841 tagaggcagt gcaaaactgg caacaacatt tgcgttctca agcttatatt aatatagttg
    15901 ataaggattt agcatgaacc cactgcttat tagtagtgga gagcctgctg gaattggccc
    15961 ggacttatgc ctggctttgg cagaaaccaa tttgcccgta gtgatattgg gagatctgtc
    16021 tttattagag gcaagagcca gtgagttaaa tctcagtatt aaatttttag agtacggccc
    16081 tcatcaaagc ttcgaaaaaa aagcaggata tttaactgta tggcctgttc catgcgctgc
    16141 accggtcatt agtggtgaat taaatccgca aaatgcggct tatgttattg aacttttgac
    16201 tttaggggct tcgctatgct ccaaaggtga gttttccgca ttagttacag cgcctgttca
    16261 taaggcaaac attaatgcag ctggtattac gtttactggc cacacggagt tttttgctga
    16321 tttctttgag gtggaaacag tggtgatgat gttggcctgt tctcatatga aagtggcatt
    16381 agtcacaacg catcttcctc tgcgtatggt gcctgatgcc attaactctt tgcttatcat
    16441 aaaggtaatt caacaattac atcattcatt aaaacatgat tttggaattc aaagtccaaa
    16501 aatcaacgtg gcaggtctga atccgcatgc tggagaatca ggatatttag gtcgagaaga
    16561 aattgaaatt attactccag ccttaaatac tctaaaaaac caggggatcg atgtgctagg
    16621 gccgttacca gcagatacga tgtttataac aaacaatatc aatcactgtg atgcttatgt
    16681 ggcaatgtac catgatcaag gactgccagt cttaaagtat gctggattta atgaagcagt
    16741 aaatataact ctcggattgc cgataattcg tacttctgta gatcatggaa cagcattaga
    16801 attagctggt aagaacaaag ctaaccctgg gagtatgctt gctgcggtta agatggcgaa
    16861 agatatggct ttaacaagga taaaatgaaa tgtctctgat tagcctgata gcagccatag
    16921 atgaaacagg aggattgggt attaacaacc aattactctg ctatttgcca gccgacttgc
    16981 agcactttaa atcgattacc atgggtaagc ctattatcat gggcagaaaa acttatgaat
    17041 cgataggcaa acctcttcca ggcagactaa atattgtgat tagtcaatcg attcagtcaa
    17101 taccgggtgt tgttgttgtc gattctttgg gccaggccat aaaagaaacc gctgacgcat
    17161 cagaggtaat gatcattgga ggagccaaga tctattctca agccatatca atagctaatc
    17221 gcttatatat cactcggata caccatcaat ttatggccga tgtatttttt ccaaaaatca
    17281 atgaattggt atggagctgc cgaagcaagg agtatcgaca gcatgataac aaaaatcaat
    17341 acgatatgac attttgcata tatgaaagaa tatcaatttg atagctatca ataatgataa
    17401 tgtctgtttt gtgagggatg tctgtgcact ggtggctgag tccttacaat tatttcagtt
    17461 ggcctgctca gtattctata attgtccgtg ataatttccc gaccaccata agaaatatta
    17521 caatgtgagt aaccaggcca tgttttccct ggctgaatac ttccattgaa gttactttga
    17581 cagacaaata aaggattacc attggtatct ttacccatta acaatgcatg ctctacgttg
    17641 ggttcccagc tgtatcgccc aaattcattt tgattcggta tggtaaactg gctaagaacg
    17701 tattctttac caccataagg tacgttgcat cgaccatatc ctgcccatgt ttttccaggc
    17761 tgtatactgt taaacagttt tgccttacac agatacaatg cattgccatt cgtgtcagtt
    17821 ccagttcgta acgcattagc taacggtgaa acaggatgtt gatggtaatg agtattggcc
    17881 caggcaggat taagaaagaa tatcatcaat aaaaggtata caagtttgga catatcaact
    17941 ctctaaaaat ttaaagagcc gcaagcataa cgcaagaaaa aaatatgcgc aagatgaata
    18001 cttaaagatc gatatggttt atctattgta acccaaagaa aaacaattaa aaaaaattaa
    18061 aaaagtattt gacagtgatg ctgaaatgag tagaatacgc cctacgccac gagggcgggt
    18121 tcattaagaa gagagaagac aaactgtgtg ggcactttga gatactttga gtgctttaga
    18181 gagataaaga aatgagctag cgagagctag tgccggaatt gaactgaaga gtttgatcct
    18241 ggctcagatt gaacgctggc ggcatgctta acacatgcaa gtcgaacggc agcattgtct
    18301 agcttgctag acagatggcg agtggcgaac gggtgagtaa cgcgtaggaa tatgccttga
    18361 agagggggac aacttgggga aactcaagct aataccgcat aatgtctgag gacgaaagct
    18421 ggggaccttc gggcctggcg ctttaagatt agcctgcgtc cgattagcta gttggtgggg
    18481 taagggccta ccaaggcgac gatcggtagc tggtctgaga ggatgaccag ccacactgga
    18541 actgagacac ggtccagact cctacgggag gcagcagtgg ggaatattgg acaatggggg
    18601 caaccctgat ccagcaatgc cgcgtgtgtg aagaaggcct gagggttgta aagcactttc
    18661 agtggggagg
//
LOCUS       DAXLDT010000057        18667 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXLDT010000057 DAXLDT010000000
VERSION     DAXLDT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18667
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.057"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2174
                     /locus_tag="ACF2SP_001563"
     CDS             <1..2174
                     /locus_tag="ACF2SP_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2359352.1"
                     /translation="QTNKPTLNSNLKTNTDGINPIDITSQFLTEQINDKSKNTSDYVY
                     AIYRYMENIIAQIPVSVYWMNKNHVYMGCSNNMVKLLNLNSRYDIVGKTYEDLYHEKS
                     TSYYKKADKIVMDKGIPLSLEEPFYCPDGTTKETYLSNKVPLRDLEGKIIGMLGVSVD
                     ITDRKKMELELSKAKEAAEEANNVKTEFIANMSHDLRTPLSGVVGMAELMKEQVTDPV
                     QVQYAEWIFDCSQQLLSLLDGILDIVSLGNVKEDELKITTFDINECIAELVHLEMPSI
                     KLKQLDLIVNIDKKIPRLIKTDRIKLHRIILNLLGNAIKFTDSGYIAIDVKLLELNNE
                     SGLIYFAISDTGIGIPENQQTKIFDRFHRIIPSSIGKYKGHGVGLHIVQSYVNLLGGE
                     IQVNSEPGVGTTFYFELPIQIEKENEVTKGHYKLEKNIIPVPKKEKILDKNSKNEFLY
                     NSPNILILEDNLVARRTIESLATQEKCRFVSVAKGKEAIRLAKTNEFDLIITDIGLPD
                     IEGHVVAKLIREWELSQSKPPIPIIGLTAHVKEKSKEICLKSGMDDVFNKPISRQLLQ
                     YILSRFIPSKNKNPSSCSSKKTTSSEQIIEADLPESESNFFNLDAYPLLDIHNAVSIM
                     GSLKLVREILPLMIGEETQKDVESIEKAFLIKDWETVERIAHKMKGGAANCGAVRMKY
                     ACQYLERYQKMGYTKLLEPLYQQLIKVVEDTQKNIHEWLKH"
     gene            2346..3254
                     /locus_tag="ACF2SP_001564"
     CDS             2346..3254
                     /locus_tag="ACF2SP_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2359353.1"
                     /translation="MSDEISKEYFNETLGIHQEYLGILNCLPDIVYWVDKDCLLKGCN
                     QNFIKLLGLKKIKDLKGTPYEQMAKYTGWPEKNIEAFKLDDMKVLFSGEPQWDVEEGP
                     LFNKDKEVSYYRTRRVPLFDKDNKVHGLVVVLTDITKFKKMEEQLQKVATKEKIKPSP
                     AKRIEEPKILMVEDNFVAQKVEEALLKELQCQVDIADTGDKALNLFDPGKYDMVFMDI
                     GLEDTSGYLVAKKIRQKEQNTNYHVPIIALTSYQADVVKYDVEDYFMDGVLTKPLTSE
                     QADQLIKHFVHKEDIMVSGLKSAREN"
     gene            3536..4297
                     /locus_tag="ACF2SP_001565"
     CDS             3536..4297
                     /locus_tag="ACF2SP_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFD2359354.1"
                     /translation="MIWDPERYVLGNYIQNKINDVFLKTIHSKLSGNILDIGCGDGHY
                     TSLLAGKVKHGQVLGIDSSEQMIMHANQQWARTGLSFEVHNIEEFHQPQSFDLALSFW
                     CLHWTNIHISFPNIFHLLKREGKLYAVMSSFSDHSILQTWKELAKQNLYRDLTEQYIS
                     PINEQYFYGVINLLNRLPFKRIKLDLKTCRVHLPHIDYYKNLLLTMPFIKRVPSEITD
                     TLVEDMLKAFQTICDRKYGGQLYYETRPIFLEAIK"
     gene            4322..5356
                     /locus_tag="ACF2SP_001566"
     CDS             4322..5356
                     /locus_tag="ACF2SP_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFD2359355.1"
                     /translation="MHLNFNTTPGMNIPMVIANQDNLKASPTPLLHFISEYHDEIIQK
                     LNQYGAIVLRGFSCQEENCFSKAIELCALGTRCSTSDYDLPRTVLANEIYTSSDLPAH
                     IPLPLHHEKPRSPKPPNHIYFCCIIPPQEGGGTIFANAEEIWIDMPQDIQNKILEYGV
                     QYKQFFHGQSVKYRVLRKILGNHCARSWVDYFGTEDKTQIEQNLTQKQVVWDWINHGN
                     DLIILNYLPGALKHPLTDKIAWFNSSAYLNYYSNLNYGELKNLRSFKYWASRYLILKD
                     MLPMICHYGNGQEFSAKEISEINQVIQRHTRVFHWQKGDFMIVDNFTFMHGKQAHVGE
                     RLLYSCMTAY"
     gene            complement(5336..5596)
                     /locus_tag="ACF2SP_001567"
     CDS             complement(5336..5596)
                     /locus_tag="ACF2SP_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359356.1"
                     /translation="MPGYAQKLFVWQRVIIKTTITEREFEYKQINLEGLFTLSIARIQ
                     QDFVWEYHIRSLTVAARIMVQTLNRDCQGADKNNPALISRHA"
     gene            5614..16959
                     /locus_tag="ACF2SP_001568"
     CDS             5614..16959
                     /locus_tag="ACF2SP_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2359357.1"
                     /translation="MVNVTMKNKTKDLLNEPIAIIGMNCQFPGIDSDIEDVSAFYDML
                     MKGQTPIKDVPENRWNIEEYYSADRKKADKIISKKGGFLDNTHLFDAAYFKISSAEAK
                     QIDPQQRLFLEVAIRALNDANIPLDSLKDSNSGVYCGISTHEYSQLNYKDHIKFNAYT
                     PIGIANSAAAGRLCHFLNLKGPSMAVDTACSSSFTALYLAATALRNQQCDMAIVGGVH
                     LSLCPESFIGLTKANMLSATGQCSSFDSKADGFVRSEGCGVVIVKRLSDAIRDNNKIY
                     ALIKSMVINQNGDGTGLAAPSTNAQIAMHQAALEHAHLTAGEIDYIETHGTGTTVGDP
                     VEFNAIQSIHQGHHSAQKPLIVGALKSNIGHTISSSGIASLIKALCSLQNEAIPPNLH
                     YSNPNNLIDPESIPALLPVEAIPFPKLKNKKRYIQVSNFSFTGTNVSVILEEAPELES
                     HESKQDNNEPKCFVASANSELSLKQLMSRYEHYLKNSSSTLGDICHTLINCRDHYKFR
                     CAIIVNDKKELIKKIESKDYEIRKVVLQNNPRTVLNDAKAIYEAYLSGINIITDDENT
                     AYNKVDLPLYCFDRKPYWHEVRKQVKNDHWLDSLYQQPMAQQVDAIKAELASQIKDLL
                     KKDSIDPHQDLETLGLTTSLLESLDQILQDMFSPRYKVPSLQSLRYLTLDKLARHLQK
                     MIMPPVVYRQPSINVLNVEPIAIIGMSCRFPKAANVDEFLSLLEKGESGMIDIPLERW
                     DNEKFYDSDVDALGKLYIKQLGFIDHVNHFDAEFFNISPREAKLMSPQLRVFMETSYH
                     ALEDANLSLDAVKDTNTGVFVGVGTNEYPRVLAYQGVTLEDLNIYFATGNVLNALAGR
                     VAYSFDFHGPIQAIDTACSSSMTAIHNACLSLQSGDCNMALAGGVNIILSPDSNITLS
                     KARMLSPDSRCKTFSEDADGYGRSEGCGVVVLKRLSDAVKDNDNILAVIKGTSINSDG
                     KSGGFTVPNGAAQEEVILSALAKANLTPGDIDFVEAHGTGTPLADPIEVNTLTKIFSE
                     HHNKENPLYISSVKTNIGHSESASGVASVIKTVLSLKTKTIFKHLNFKKLNPAIQLIN
                     TVIPLDKTDWAKKQGLRCAGVSSFGFSGANAHAIIQEAPEDKKEARALPEESLLVLSA
                     KSKTSLELLLASYQNYLSNTREEFADICYTAATCRTHFMYRVAIRAQNATQAANIIKN
                     NGYTICHITKEKEWAQQPDTLAELQTAYLDGFLINWSEFYRSFGHHFAKVKLPLYEFE
                     RIEHWFGDKNKLKDAPLPKDWCFQIQWQNEPCDKSNRKLRGNRWLLIGPRHLADGFKA
                     QGLEIIYEDEEYSRDKLEGIIFAESLHLAPPDIKANIDFQKKTLKKLLNLIKELDNQS
                     IDLQLLILTSNGIAELANDKHPVNISNSPLIGFCKTLALELPKFKTILIDMDKIEEDK
                     YPTQVIEEINYNHGSRYEHIVAYRGGERLASRLKKTPLEDRRRLLHGVKGRYLITGGC
                     GGLGLVTAQSLLSSGARELILTSRNIDKPRIKEAIYKIKSHYPGANIRVVSLDITDRE
                     SLKNLLSELNTDGQLKGIIHAAGAAIKAPLLEHQDEDVDYLFSAKVKGGWYLHELSLD
                     CNLDFFVVYSSVSSVFGSNKESVYSGTNSFLDALIAERQRMGLPGTAVQWGPWGEVGM
                     AQKRSRDEGLKQALISNAQGHVIIKCLINDEFKHATIISPEYLKFMLDFVPKPLPGFY
                     GQLDEELEKIPQQHHQSPAKNLSPWLNQYLEISEEERFKACKAMVSDICTGILELTEN
                     EDLNEDEGFFELGFDSLMITELASELKKKLEPFLKVTVTIGFNYPSISKLAKYIESEL
                     DKNLVKIQSPKSAPKPIDDGIAIIGMSCLLPNAPDIAAFEKLLEEGLSGIKDIPNDRW
                     DNNQYYDPNMEAPGKSYVTKLGLIENIKYFDAAFFGISPREAKLMEPQQRVFLECCYK
                     ALENANYPSESLRGSLTGVFAGVGPNEYYAQLEKSGFSNEELSAYSITGNVLNLIPGR
                     VAYTFDFKGPSISVDTACSSSLVAIHYACQSLKNKEIDYALAGGVNILLMPESNVTLC
                     KAKALSPDGQCKTFDERADGYVRGEGCGVVLLKRLSDALRDNDHILAVIKGSAVNNDG
                     KTAGLTVPNGKSQEEVMKKALGQTELSSTDISYIEAHGTGTPLGDPIEVDAINKVYGH
                     QRNKENPLYLGTVKTNIGHLESASGVAGVIKTVISLQRKTIYKNLNFNKLNPNIKLDG
                     TQLALEKTNWNTNTKLKYAAVNAFGFSGTNAHVILAEFPKEEKPKTSKPSQKHLLVIS
                     AHSKTALDNLTQRYQQYLETTEHHFGDICFTAATCREHYHYRLALTAENAVEASRMLG
                     AGQFALSYHENITLDLQNDRALNSLLMDYLQGKPVDWKSYYQSLKGDFTKVLLPNYTF
                     DRSEYWLDKKNNDSMATHKDEQHPLLGQMLAMPGNEYLFHQKLDLENLAYIKQHSVFE
                     KVIFPATAYIESGLAAAKSIFQSSAFQIEKFNIERPLHPKQGQEFQLQVKPKMDGQYK
                     INLFAKQENDWQTFCEMEIHAKPTAARESIHIDVLKSSFGDRVELSDIYEQFQKSSLF
                     YGDEFHVLQEGYVQANSVLAKVTLTKASQGQDYYYHPVLLDGAMQSILLLSVKNDENK
                     NFNAENITYIPYAFTRMTVFQEAPRSLWVHLTKQNTEDASDLCVNMNLYEQSGLLIAQ
                     IAGLKLRRVTRSHFVSYDAALSHLYYMEWSPVPFKTLPHQQEIPDLLAITKNPGKAKE
                     ILDGLNYQLVERIDDYTNIENSNIIFFYEQGQFHDLFHCCQILFRLRPKRFILVTEHA
                     YAIQDHDQVNPYHTMACSFWKSFRNEYEPNKNYAIDLGSRSRLAKALMYLLNADTHDT
                     QIAIRESLYLPRLKKKQLVSNPDPERLIDGNATYLITGGTGGLAKPLMEYLMRRGARH
                     IAITSRTHYPDDINALIDKAKQNKIDIKHYMVDAGNYQKMEQVIGEIQQSPNPLKGVF
                     HLAGLIQDGLIVNLNDEAMQKVISAKMDGALILHQLTQNIPLKWFVMFSSSASLLGAR
                     GQANYAAANGFLDGLAHLRRQQGLPAISINWGPFHTTGMAANLTHTLQHYGFLPLDKD
                     NIDILDVLLPSQLPQISPCPMNWDVYCKHTPKHMELSGLVKAKPLPDQSFLNALRQHS
                     KEESISILSQALREIAADVLALDDSEHISNHHDLFSMGLDSLMSMDIRNRIYDKLQCQ
                     TLSLPIEYFINTPTINKIARHISDELDHVFDSHSDYHPTENPSEREIALCDFQYVFWV
                     LNKLDYHFNIGTQIQLHGRINKDYVQRAFEFVVNQNSVFWIHFNKDKPTQKLHKVGQF
                     ELIYQDISLNHETKALNHEFQNNILRAIPLTKQPLIRVYLYKINNDLHELHIVIPHII
                     VDDASCEMVFCQFKNNYEKLTLGKELVSTPEKGSYFSYVKQNNNHYQKNLKNKIDFWQ
                     NYNKGFKMLSFGNRYHLSDTSKQTKHLFHYPIDPQHAEQFINWHKAQNINVSTGLIAA
                     CQIVFYRLSRQNKIPIILIHSGREGGEYKSTLGLFSEYKRINLTLNENSQFIDCIHSI
                     EEQLLKTAPYQKCSHLIKDSGLKGSSLSFGQYLTLALNKMFMLKHFKESKLHSIIIDY
                     YLKYLSRLVSFKKNISMKQRLNQLFKLDMSLQKPERLRVLVSVTPSFFTKERPDMSFA
                     NLDYSYPNHFGCMDRPIGNRTLWIYFSKNQDGEYQISINAPLTIECKNKIGIGLSQFI
                     TTFVENNNCNIAELIQAMEDSSIAS"
     gene            complement(16962..17363)
                     /locus_tag="ACF2SP_001569"
     CDS             complement(16962..17363)
                     /locus_tag="ACF2SP_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFD2359358.1"
                     /translation="MKSLRMAGVFFLFLFSFVSIHVYAAPNTGASNAEMKDVCQLAGG
                     TFTSTIEGNWACCWDNWGCYGCVQGVCKMKCHTQRCRRANGQSAVLQPGEVKIKGKFG
                     TDIVVPKVSTQPSSSNPKQIKNNNTTVNQAQ"
     gene            complement(17590..18543)
                     /locus_tag="ACF2SP_001570"
     CDS             complement(17590..18543)
                     /locus_tag="ACF2SP_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFD2359359.1"
                     /translation="MTSISEWWMWLGFLAFVLIMLFVDLFVFGGRKAHRVSTKEALSW
                     TILWFTLAIIFNFLLWWYLVENFTFSVANEKALEFFTGYLIEKSLSFDNIFVFLILFE
                     YFAIPAKYQHRVLIYGVIGAIVMRLILILFGIWIVNQFHWILYIFGFFLMITGIKMFI
                     FADKKPELAKNPILHWMRSHLRITHELHGERFFLRKNQLVYVTPLFMVLVLIEISDLI
                     FAVDSIPAIFAITNDPFIVFTSNIFAILGLRALYFLLVNMHKRFYFLKYGLAFILVFV
                     GFKMLIAPWFKIPIFIALGIVMATLVFCILFSIWHRKNEVI"
BASE COUNT         6276 a         3647 c         3514 g         5230 t
ORIGIN      
        1 atcaaaccaa taaacccaca ttgaatagca atctcaaaac aaacacagat ggcatcaacc
       61 ctatagacat cacaagtcaa tttttaacgg aacaaataaa tgataaaagt aaaaatactt
      121 cagattatgt atatgctatt tatcgctaca tggaaaatat tatcgcacag attccagtta
      181 gtgtttattg gatgaacaaa aatcatgtgt acatggggtg tagcaataat atggttaaat
      241 tattaaatct caattcaagg tacgatattg ttggtaaaac ttacgaggat ctatatcacg
      301 aaaaatcaac ctcatactat aaaaaagcag ataaaatcgt tatggataaa ggtatcccat
      361 taagtctgga agagccattc tattgccccg atggaactac gaaagagact tatttgtcga
      421 ataaagtccc tcttcgtgac ctggagggta aaattatagg tatgctcggg gtatcagtag
      481 atattactga tagaaaaaaa atggagttgg aacttagtaa agccaaagaa gctgctgagg
      541 aagcaaacaa tgtaaagact gaattcatag ccaatatgag ccatgatctc agaacccctc
      601 taagtggtgt tgtgggtatg gctgaactta tgaaagagca agtaactgat cctgtacaag
      661 tacaatatgc ggagtggatt tttgattgca gccaacaatt attgagttta ttagatggca
      721 tacttgacat cgtatccttg ggaaatgtta aagaagatga attaaaaata acaacctttg
      781 acatcaacga atgtattgca gaattagttc atcttgaaat gccgtccatt aaactgaaac
      841 aacttgattt aatcgttaat attgataaaa agattcctcg attaataaaa accgaccgta
      901 taaaacttca tcgaattatt ctcaatcttt taggtaatgc cattaaattt accgattctg
      961 gttatattgc aattgatgtg aaattacttg agctaaataa tgagtcagga ttaatttatt
     1021 ttgcaatatc tgatactggc attggtatcc ccgaaaatca acaaacgaaa atatttgaca
     1081 gatttcatcg tattatcccg tccagcatag gaaaatataa aggtcatggc gttggtttac
     1141 atattgttca atcctatgta aacctgctag gtggagaaat acaggtaaac agtgaaccgg
     1201 gagtaggaac taccttttat tttgaactcc ctattcaaat tgaaaaagaa aatgaagtga
     1261 caaaaggtca ttataagctt gaaaaaaata taatacctgt acctaaaaaa gaaaaaattt
     1321 tagataaaaa ctcaaaaaat gagtttttgt ataactcacc taatattctc atcctggaag
     1381 ataatctggt agccagacgt acaatagaaa gcttggcgac tcaagaaaaa tgccgttttg
     1441 tttctgtggc aaaaggtaaa gaagcgatac ggcttgctaa aaccaatgaa tttgatctga
     1501 taatcacaga tattgggtta ccagatattg aaggacatgt ggtagcaaaa ttaattagag
     1561 aatgggagtt atctcaatct aagccaccga taccaataat agggttaact gcacatgtca
     1621 aagaaaaatc caaagaaata tgcttgaaat caggtatgga tgatgtattc aacaaaccaa
     1681 tcagtcggca gcttttacaa tatatactga gtcgattcat tccatcaaaa aataaaaacc
     1741 caagttcatg ctcttctaaa aagacaacat catcagagca aataatagag gcagatttgc
     1801 cagaaagtga aagtaatttt tttaacttgg acgcctatcc tttactcgat attcacaatg
     1861 ctgtttccat catgggtagt ctcaaattgg ttcgagagat attgccactt atgataggtg
     1921 aagaaaccca aaaagatgtt gaatccattg aaaaagcttt tcttataaaa gattgggaaa
     1981 cggtagagag aatcgctcat aaaatgaagg gtggtgctgc gaattgcggt gctgtacgta
     2041 tgaaatatgc ttgccaatat ttagagcgct atcaaaagat gggatatacc aagcttcttg
     2101 agcctcttta ccaacaactt ataaaggtcg tggaagatac ccaaaagaat attcatgaat
     2161 ggctaaaaca ttgactctca ctagaaacat caatggtatt caataattaa gcccccagaa
     2221 aaaacctcaa atataatact tatcaatcaa tttgaatatt ataaatacac attaattaac
     2281 tttgctataa ttaatttaaa taacgccaaa ttggattcgg tgtaaccttt gggaggaagc
     2341 agtaaatgtc tgacgaaata agtaaagaat attttaatga gactctcggc atccatcaag
     2401 aatacctggg tatccttaat tgcctaccag atatcgttta ctgggtggac aaagattgtt
     2461 tattaaaagg ttgtaatcaa aacttcataa aattacttgg tttaaaaaaa ataaaagatt
     2521 taaaaggaac tccttatgaa caaatggcaa aatacactgg gtggccagaa aaaaatattg
     2581 aagcatttaa actggatgac atgaaagtgc tcttttcagg agaaccccaa tgggatgtag
     2641 aagagggacc tttatttaac aaggataagg aagtgagtta ttaccgaact cgtcgcgtac
     2701 cattatttga taaagacaat aaagtacatg gattggtagt tgtactaact gacatcacca
     2761 aatttaaaaa aatggaagag caattacaga aagtagctac aaaagaaaag ataaaaccta
     2821 gcccagccaa acgaatagaa gaaccaaaaa tattaatggt cgaagacaat tttgttgcac
     2881 aaaaagttga agaggcattg cttaaggaac tgcaatgcca agttgatatc gcggacacgg
     2941 gagataaagc attaaatctt ttcgacccgg ggaaatatga tatggtcttt atggatattg
     3001 gtctcgaaga tacttctggc tatttggtag caaaaaaaat tcgccaaaag gaacaaaaca
     3061 ccaattatca tgtgccaatt attgccttaa catcttacca ggctgatgtg gtgaaatacg
     3121 atgttgagga ttattttatg gatggagtgc tcactaagcc tttaacaagc gaacaagcag
     3181 accagttaat caaacacttt gttcataaag aagatattat ggtttcaggc ttaaaatcag
     3241 caagagaaaa ttaagatcaa cttgcttagc agacaatatc tgatttttag tttgttcgca
     3301 tgaaacaaaa tgaaacattg ccaaaacctg gtaaatagag aaaaggttaa attggcacat
     3361 ccgtgcacta caggattaaa gtacaacctc gtcggcgagg actgtatcct cataaaaaca
     3421 agacacatcc cgcataatgt gtgagaatag atttcagcca tgccaaagat atttgtaatc
     3481 gctggtttct tgggtatatc ttcccttaca atgattattg atactggaga atgaagtgat
     3541 atgggatcct gaacgttatg tgcttggaaa ctatattcaa aataaaatca acgatgtatt
     3601 tctaaaaaca attcacagca agttgtctgg aaatatttta gatattggtt gcggagatgg
     3661 gcactatacc agcttgttgg ctggcaaggt aaaacatggc caagttctgg gaattgatag
     3721 ctctgaacaa atgataatgc acgccaatca acaatgggca cgtacggggc tatcttttga
     3781 agttcataac attgaggaat ttcatcaacc gcaatctttt gatttggcgc tctcattttg
     3841 gtgtctgcat tggacaaata tccacatttc ttttccaaat atttttcatc ttctaaaaag
     3901 ggaaggcaaa ttgtatgctg ttatgtcttc cttttccgat cacagcattt tgcaaacctg
     3961 gaaagaattg gccaaacaaa acctgtatag ggatttaaca gaacaataca tctctccaat
     4021 caatgaacag tatttttatg gtgtgattaa tcttctcaac agattgcctt ttaaacgaat
     4081 taaacttgat ttaaaaactt gccgtgttca tttgccgcac attgattatt ataagaattt
     4141 acttctaacc atgcctttca taaaaagagt tccttctgaa ataacagata cactggttga
     4201 ggacatgtta aaagcatttc aaactatctg tgacaggaaa tacggtggtc aattatatta
     4261 tgaaactcgc cccatatttt tagaggctat caaataactc aaggtgttag gaggcaatac
     4321 cgtgcattta aattttaata caactcctgg catgaacata cctatggtca ttgcaaacca
     4381 ggataatttg aaagcaagcc ctaccccttt gttacatttt atttctgagt atcatgatga
     4441 gataatccaa aagctcaatc agtatggagc aatagtatta agaggatttt cctgtcaaga
     4501 agagaactgc ttttcgaaag caatcgaatt gtgtgcctta ggcacgcgat gttcgacaag
     4561 cgattatgac ttgccaagaa ctgtactggc aaatgaaatt tacacatcaa gtgacctgcc
     4621 ggctcatatt cccttaccgc tgcatcacga aaaaccccga tcaccaaaac cacctaatca
     4681 catctatttt tgctgtatca tcccgcctca ggaaggcggt ggaacaatct ttgccaacgc
     4741 tgaagaaata tggatagata tgccccaaga cattcagaac aagattcttg aatacggcgt
     4801 ccaatacaag caattttttc atgggcaatc tgtaaaatat cgtgtattaa gaaaaatcct
     4861 gggaaaccat tgtgctcgaa gttgggttga ttattttggc acagaagaca aaactcaaat
     4921 agaacaaaat ttaactcaaa agcaagtagt atgggattgg ataaatcatg ggaatgattt
     4981 gataatacta aattatttgc ccggagcact aaagcatcct ctaactgata aaatagcctg
     5041 gtttaattca tcagcttatt tgaattatta ttccaatttg aattatggtg aattaaaaaa
     5101 cctgcgttca ttcaaatatt gggcaagccg gtatcttatc ctgaaagata tgcttcccat
     5161 gatctgtcat tatggtaatg gccaagagtt ttctgcaaaa gaaatatctg aaattaacca
     5221 ggttattcag cgtcatactc gtgtttttca ttggcaaaaa ggagatttca tgattgtgga
     5281 taattttact ttcatgcatg gcaaacaagc tcatgttgga gagagattat tgtattcatg
     5341 catgacggct tattaatgct ggattatttt tatccgctcc ctgacagtcg cggttcagtg
     5401 tttgaaccat aatccgagcc gcgactgtca gggagcggat atggtattcc catacaaaat
     5461 cttgctgaat tctggctata cttaaggtaa atagaccttc caggttaatt tgtttatact
     5521 caaattctct ctctgttatg gttgttttta ttataactct ttgccagaca aataattttt
     5581 gtgcgtagcc aggcactttc aggagcattg taagtggtaa acgtaactat gaaaaacaaa
     5641 accaaagacc ttttgaatga acccattgcc atcattggga tgaattgtca atttcctggg
     5701 attgattctg atatagaaga tgttagtgcc ttttatgaca tgctgatgaa agggcaaact
     5761 ccaatcaaag atgtgcctga aaatcgctgg aatattgaag aatactatag tgctgacaga
     5821 aaaaaagcag ataaaattat tagcaaaaaa ggcggttttt tagataacac ccatttattc
     5881 gatgctgctt attttaaaat atcttctgcc gaagcgaagc aaatagatcc ccaacaacgc
     5941 ctttttctcg aagtggctat tcgagcttta aatgatgcca atatcccgct agactcttta
     6001 aaagattcta acagcggagt ttattgtggc atttccacac acgaatacag ccaactgaac
     6061 tataaagacc atatcaaatt taatgcctac acacccattg gaatagcgaa cagtgctgcc
     6121 gctggcaggc tctgccattt tttgaattta aaaggaccca gcatggctgt tgatacagca
     6181 tgctcttctt ccttcactgc actctatcta gcggccaccg cattgagaaa ccagcaatgt
     6241 gatatggcaa ttgtcggggg cgtccatttg agtctctgtc ctgaaagttt tatcggatta
     6301 accaaagcca atatgctgtc agcgacaggt caatgcagca gctttgacag taaagcagac
     6361 ggctttgttc gtagcgaagg ttgcggagtg gttattgtta aacgcttaag tgacgccata
     6421 cgagacaata acaaaatcta tgcactcatc aaaagcatgg ttattaatca aaatggcgat
     6481 ggaacaggcc ttgcagcccc gagtactaat gcccaaattg ccatgcacca agccgcattg
     6541 gaacatgccc atttaactgc aggtgaaatt gattacattg aaacccacgg aacaggcaca
     6601 acagtaggtg atcccgtcga atttaatgcg atccaaagca ttcaccaggg gcatcactct
     6661 gctcaaaaac ccttgatagt aggagcactg aaaagtaata taggccatac tatttcatca
     6721 tcaggcattg cttctttaat taaagcactt tgctctttgc aaaatgaagc gattcccccc
     6781 aatttgcatt attcaaaccc gaacaacttg attgatcctg aaagtatccc tgcgcttcta
     6841 cccgttgaag caattccttt tcccaaactc aaaaataaaa aaagatatat ccaggtatct
     6901 aatttcagct ttacgggcac gaatgtaagt gtcattttgg aagaagcgcc tgagttggaa
     6961 tcacacgagt caaagcaaga caacaatgaa ccaaaatgtt ttgttgcctc agccaatagt
     7021 gaactctcat tgaaacaatt gatgtcacgc tacgaacact atttgaaaaa ttcttcctca
     7081 accttgggcg atatatgtca taccttgatc aattgtagag accattacaa atttcgctgc
     7141 gccatcattg taaacgataa aaaagaactc attaaaaaaa ttgaatcgaa agactatgaa
     7201 ataagaaagg tagttctaca aaacaacccc agaacggtat tgaatgatgc caaagccatt
     7261 tacgaagctt acttatccgg tattaacatc attactgatg atgaaaatac tgcatacaac
     7321 aaagtagatt taccgctcta ctgttttgac agaaaaccct attggcatga agtcagaaaa
     7381 caagtcaaga atgatcattg gctggattct ttatatcaac aaccaatggc acaacaagtt
     7441 gacgccatca aagcagagct tgcctctcaa atcaaagatc ttttaaagaa agacagtata
     7501 gatccacatc aggatttgga aactttgggt ttaactacct ccttattgga atccctggat
     7561 caaatccttc aggatatgtt ttccccccga tataaggtgc cttctctcca atccttgcgc
     7621 tacttaaccc tggataaact ggcaaggcat ttacaaaaaa tgatcatgcc gcctgtcgtt
     7681 taccgacaac catctattaa tgtactcaat gttgagccca tcgccattat cggtatgagt
     7741 tgtcgtttcc ccaaagcagc caatgtggat gaattcctgt ctttgctgga aaaaggcgaa
     7801 agtggcatga tcgatatccc cttagagcgt tgggataacg aaaaatttta tgactcggat
     7861 gtcgatgcat taggaaaact gtacatcaag caactgggct tcattgatca tgtcaaccat
     7921 tttgatgcgg aattttttaa tataagcccg cgtgaggcta agctcatgtc acctcaattg
     7981 cgtgttttta tggaaaccag ttatcacgca cttgaagatg ccaatctctc tcttgatgcg
     8041 gtcaaagaca ctaatacagg agtttttgtc ggagtgggga ctaatgaata tccccgagtt
     8101 ttagcttatc aaggtgttac cttggaagat ttgaatattt actttgcgac ggggaatgtt
     8161 ttaaatgcgc tggccggtcg tgtagcctat tcctttgact ttcatggtcc aatacaagca
     8221 atagataccg catgctcatc ctccatgaca gccatacaca atgcctgcct gagtctgcaa
     8281 tccggtgact gtaatatggc tcttgctggc ggtgtgaaca tcatccttag ccctgattct
     8341 aacattactc tttcaaaagc cagaatgctc tcacctgaca gccgctgcaa aaccttcagt
     8401 gaagacgctg atggctatgg tcgcagcgaa gggtgtggtg tggtggtttt aaaacgttta
     8461 agtgacgccg ttaaagacaa cgataatatt ctggcagtaa tcaaaggcac ttccatcaac
     8521 agcgatggta aaagtggtgg ttttactgta cctaatggtg ctgcgcagga agaagtcatc
     8581 ctaagtgcat tggcaaaagc taacctgaca cctggtgata ttgattttgt cgaagcccat
     8641 gggactggca caccattagc tgatcctatc gaagtgaata cgctgaccaa gatttttagt
     8701 gaacatcaca acaaagaaaa cccgctttat atcagttcag taaaaaccaa tattgggcac
     8761 tcggaaagcg catccggtgt agccagtgtt atcaaaaccg ttttaagcct gaaaaccaaa
     8821 acaatattta agcatctgaa cttcaaaaag cttaatcctg ccattcaatt aattaataca
     8881 gtaatccctc ttgataaaac cgattgggcc aagaaacaag ggttaaggtg tgccggggtt
     8941 agttcttttg gttttagtgg ggctaatgcc catgccatca tacaggaagc tcctgaagac
     9001 aaaaaagaag cacgcgccct tcccgaagaa tctctgctcg ttttatcagc aaaaagtaaa
     9061 acctctctgg agttattact ggccagctac caaaactatt tatccaatac ccgggaagaa
     9121 tttgccgata tctgctacac agcagcaact tgccgcactc attttatgta tcgtgtagca
     9181 attagagcgc aaaatgcaac ccaggcggcc aatatcataa aaaacaatgg atacactatt
     9241 tgccatatca ccaaagaaaa agaatgggct caacaaccgg acacacttgc agaattgcaa
     9301 acagcctacc tggatggatt cctcatcaac tggtctgaat tttatcgctc ctttggccat
     9361 cattttgcaa aagtaaaatt acccttatac gaatttgagc gtatagaaca ttggtttggt
     9421 gacaaaaaca agctgaaaga tgcgcctcta cccaaggact ggtgttttca aatccagtgg
     9481 caaaatgaac catgtgataa aagcaatcga aagctgcgag gaaatagatg gcttttaatt
     9541 ggtcccagac atctggctga tggtttcaaa gctcaaggcc tggaaattat ctacgaagat
     9601 gaagagtatt ctcgagacaa actggaaggc atcatttttg ctgaaagttt gcatctggca
     9661 ccacctgaca taaaagctaa tattgacttt caaaagaaaa ccttaaaaaa gctgctgaac
     9721 ttaattaaag aacttgataa ccagtcaatt gatttgcaat tgctcattct taccagcaat
     9781 ggaattgctg aactggcgaa tgacaaacac cccgtcaata taagcaatag ccctctcatt
     9841 ggtttctgca agactctcgc tttagagctg ccaaaattca aaaccattct catcgatatg
     9901 gataaaatcg aagaggacaa atacccgaca caagtgatag aggaaatcaa ttataaccat
     9961 ggaagccgct atgaacacat agtagcttac cgtggtgggg aaaggctggc ttcccgttta
    10021 aaaaagactc cgctggaaga taggcggcga ttattgcatg gagtgaaagg acgctatctt
    10081 attactggcg gctgcggtgg tttgggattg gtaactgccc aatccttgct ttcctctgga
    10141 gcaagagaat taattctcac ctcaagaaat atcgacaaac caaggatcaa ggaagccatt
    10201 tataaaatta aatcccatta ccctggcgcc aacatccgcg ttgtcagttt ggatattact
    10261 gatagggaaa gtttaaaaaa tttattatca gagcttaata ccgatggtca gttaaaaggc
    10321 atcatccatg ctgctggtgc tgcgattaaa gcgcctttgc ttgaacatca ggatgaagat
    10381 gttgattatt tattttcagc taaagtaaaa ggcggatggt atttacatga actaagtctg
    10441 gattgcaacc tggacttctt tgttgtctat tcttcagttt cctccgtatt tggaagcaac
    10501 aaagaatccg tttatagtgg aacgaacagt ttcctcgacg ccttgatagc ggagcgccag
    10561 cgtatggggt tacccggtac tgctgttcaa tgggggcctt ggggtgaagt aggaatggcc
    10621 caaaaacgct cgcgtgatga aggactaaaa caagcgttaa tcagtaatgc acaaggtcat
    10681 gtaatcatta aatgcctcat caacgacgaa ttcaaacacg ccactatcat atctccagaa
    10741 tacctcaaat ttatgctgga ttttgtaccc aagcccctac ctgggtttta tggacaactt
    10801 gatgaggaac ttgaaaaaat accacaacaa catcatcaaa gcccggcaaa aaatctgtct
    10861 ccatggctta atcaatatct ggaaattagt gaagaggagc gatttaaagc ctgtaaagca
    10921 atggtatctg atatttgcac aggaatcctt gaactgacag aaaatgaaga cttaaacgaa
    10981 gacgaaggtt ttttcgagtt gggttttgac tcgctcatga ttactgaatt ggccagtgaa
    11041 ctaaagaaaa agctggaacc attccttaaa gttaccgtga ccattggttt taattatcca
    11101 tccatcagta agctggccaa atacattgaa tcagaacttg ataagaactt ggtcaagata
    11161 caaagtccaa aatcagctcc caagccgatt gatgacggta tagcgattat aggcatgagt
    11221 tgtttattac ctaatgcgcc agacatcgcc gcctttgaaa aactgttgga agaaggatta
    11281 agcggtatca aagacattcc aaatgaccgc tgggataata accaatatta cgatcccaat
    11341 atggaagcgc ctggtaagtc ctacgtcacc aaactgggat taatcgaaaa tattaaatat
    11401 tttgacgctg cattttttgg tattagtccc cgagaagcaa aattgatgga acctcagcaa
    11461 agagtatttc ttgagtgttg ctacaaggca ttggaaaatg ccaactaccc ttcggaatct
    11521 ttgcgtggca gcttaacagg cgtatttgcg ggcgtaggtc ccaatgaata ttatgcgcaa
    11581 ctggaaaaat cagggttttc aaatgaagaa ctcagcgctt actctatcac gggtaatgta
    11641 ctcaatctga ttccaggccg agtagcctac acttttgatt ttaaaggccc gtcaatcagt
    11701 gttgacacgg catgctcttc ctcgttggtc gcaatccatt acgcctgcca aagtttaaaa
    11761 aataaggaaa ttgattatgc tcttgcgggt ggggtgaata tactgcttat gccggaatct
    11821 aatgtaactt tgtgcaaggc aaaggcatta tcacctgatg gccaatgcaa aacctttgat
    11881 gaaagagctg atggatacgt cagaggcgag ggttgtggcg tagtattgct taaaaggctc
    11941 tctgatgcct tgcgggataa cgatcatatc ctcgccgtta ttaaaggctc agccgtaaat
    12001 aacgatggta aaacggctgg attaaccgtg cctaatggta aaagccagga agaagtcatg
    12061 aaaaaagcgc ttggccagac tgaattgtcg agcaccgaca tcagttatat tgaggctcat
    12121 ggcacaggaa ctccgttagg ggatcctatt gaagtggatg caatcaacaa agtctacggt
    12181 catcaacgca acaaagagaa ccccttatac ctgggtactg ttaaaaccaa tattggacat
    12241 cttgaaagcg catcaggcgt agcgggcgtt ataaaaaccg ttatcagcct gcaaaggaaa
    12301 accatttata agaatttgaa ttttaataaa ttaaatccca acatcaaact ggatggtact
    12361 caactggcct tagaaaaaac aaattggaat acgaatacaa aactcaaata cgcagcggtc
    12421 aatgcgtttg gttttagcgg cacgaatgca catgttatcc tagcagagtt tcctaaagaa
    12481 gaaaaaccca aaacctcaaa accctctcaa aaacatttac tggttatttc agcccactcc
    12541 aaaacggctt tagataattt aactcagcga taccaacagt atttagagac gactgaacat
    12601 cattttggtg acatatgctt taccgcagcc acttgtcgag aacattatca ttaccgtttg
    12661 gctttaaccg ctgagaatgc agttgaagcg agtcgtatgc taggtgcagg gcaatttgcc
    12721 ttgtcttatc atgaaaatat tacactggac ttacaaaacg acagagcact caattccctg
    12781 ttaatggatt atctgcaagg aaaaccggtt gattggaaat cctattatca atcccttaaa
    12841 ggagatttta caaaagtcct gctgcctaat tatacctttg accgcagcga gtattggctg
    12901 gataaaaaaa acaacgatag catggccact cataaagatg agcaacaccc tcttttaggg
    12961 caaatgcttg ccatgccagg aaatgaatat ctgtttcatc agaaactgga tttggagaat
    13021 ttggcttata tcaaacaaca cagtgttttc gaaaaagtga tttttccagc aacagcctat
    13081 attgaatcag gtttggcagc agcaaaatcc atatttcaat ccagcgcttt tcaaattgaa
    13141 aaattcaata tcgaacgtcc tctacatcct aaacaaggtc aggagtttca attacaagtc
    13201 aaacctaaaa tggacggaca atacaaaatc aatctattcg cgaagcaaga gaacgactgg
    13261 caaactttct gtgaaatgga aatccatgcc aaaccgacag cagcccggga atctatccat
    13321 attgacgtgc ttaaatcatc ctttggcgat agagtagagc tgtcggacat atatgagcaa
    13381 tttcagaaaa gctcattatt ttacggcgat gaatttcatg tcctgcaaga gggctatgtt
    13441 caggcgaaca gtgttctggc taaagtcaca ttgactaagg caagccaggg ccaggactat
    13501 tattatcatc ctgttctgct tgatggtgcc atgcaaagca tcttgttatt aagtgttaaa
    13561 aatgacgaga ataagaattt taacgccgag aatattacct acatacccta tgccttcacc
    13621 cgcatgactg tatttcaaga agcgccacga agcctatggg tgcatttaac taaacaaaat
    13681 actgaagatg ccagcgatct ttgcgttaat atgaatttat atgagcaatc tggtttatta
    13741 attgctcaaa ttgcaggcct taaattgcga agggtaacac gaagtcattt tgtttcctat
    13801 gacgctgctt taagccacct ttattacatg gaatggagcc ctgtcccttt taaaacctta
    13861 cctcaccaac aagaaatccc tgatctcttg gccattacaa aaaatcctgg caaagctaaa
    13921 gaaatattgg atgggcttaa ttatcagctg gtcgaaagga tcgatgacta cacaaatatc
    13981 gagaacagca acataatatt cttttatgaa caaggacagt ttcatgatct ttttcattgc
    14041 tgccagatct tattccgatt gcgtcccaag cgctttatac tggttacgga acatgcttat
    14101 gccattcaag accatgatca ggttaatcct taccacacaa tggcctgttc tttttggaaa
    14161 agctttagaa atgaatatga gcccaataaa aattatgcca ttgatttggg ctccaggagt
    14221 cgcttggcta aggcgttaat gtatttattg aatgcagaca cccatgacac tcaaatagca
    14281 atcagagagt ctctttactt acccaggcta aagaaaaagc aattggtcag taaccctgac
    14341 ccagagagat taattgatgg caatgccact tatcttatta caggcggtac tggaggatta
    14401 gccaaaccct tgatggaata tctgatgagg cgcggtgcca ggcacattgc cattaccagc
    14461 cgcacccact atcccgatga cattaatgca ttaatagata aggccaagca aaataagatt
    14521 gatatcaaac attacatggt tgatgccgga aattatcaga aaatggagca agtgattgga
    14581 gaaatacaac agagccccaa tccattgaaa ggcgtatttc atttggcagg tttgatacaa
    14641 gatgggctca tcgtcaattt gaatgatgaa gcaatgcaaa aagtaatcag cgcgaagatg
    14701 gatggcgctt taattttaca tcaattaacc caaaacatcc cattaaaatg gttcgtcatg
    14761 ttttcttcat ccgcttctct gttaggtgcc agaggtcagg caaactatgc cgcagctaat
    14821 gggtttttag atggtttagc ccatttaagg cgccagcagg gcttaccagc aatttctatc
    14881 aactggggac ctttccatac cacaggaatg gcagccaatt tgactcatac tttacagcat
    14941 tatggttttt taccattaga taaggacaac attgatattc ttgatgtgct cttgcctagc
    15001 caattgcctc aaatttctcc ttgccctatg aattgggatg tttactgcaa acatacaccg
    15061 aaacatatgg aattatctgg gctggttaag gcgaaaccac tacctgatca gtcttttttg
    15121 aatgcacttc gacaacattc caaagaagaa agcatctcaa tattgtcaca agcattacga
    15181 gaaattgcag cggatgtcct ggctttggat gacagcgaac acatttcgaa ccaccacgat
    15241 ttgttttcaa tgggtctgga ttcactgatg tcaatggata tcagaaatcg catttatgac
    15301 aaattacaat gccaaacact aagcttaccg attgaatatt ttattaatac tcctaccatt
    15361 aataaaatcg ccagacatat ttctgatgaa ttagaccatg tttttgatag tcactctgac
    15421 taccacccta ccgaaaatcc gtcagaaaga gaaattgcat tatgtgattt tcaatatgta
    15481 ttctgggtgc ttaacaaatt ggattaccat tttaatatcg gtacacaaat acaattacac
    15541 ggccgaatca ataaagatta tgtccaacgg gcttttgaat ttgttgtgaa tcaaaacagt
    15601 gtcttctgga tccattttaa taaggataaa ccaacccaaa aattgcataa agttggacaa
    15661 tttgaattaa tttaccagga tatttctctg aatcatgaaa ctaaggcgct gaatcacgaa
    15721 ttccaaaaca atatattgcg cgctattcca ctcaccaaac aaccattgat tagagtgtat
    15781 ttgtataaga ttaataatga cttgcatgaa ttacacatcg taatacccca tattattgtc
    15841 gatgacgcat catgcgaaat ggttttttgc caatttaaaa acaattatga aaaactaact
    15901 cttggaaaag aattagtatc caccccagaa aaaggctctt attttagtta tgtgaaacaa
    15961 aataataatc actatcaaaa aaacctgaaa aataaaattg atttttggca aaactataat
    16021 aaaggcttta aaatgctgag tttcggcaat agatatcatt tatccgatac ctcaaaacaa
    16081 acaaagcact tatttcatta tcctattgat cctcaacatg ccgaacaatt tataaattgg
    16141 cataaagccc aaaatatcaa tgtcagtact ggattaattg ccgcatgcca aattgttttc
    16201 tacagactaa gccgccaaaa taaaataccg atcattttaa ttcacagtgg tagagaaggt
    16261 ggggaatata aatcaaccct gggtttgttc tcggaatata aaaggataaa tcttacctta
    16321 aatgagaata gccaatttat tgattgtatt cattcaattg aagagcaact cttaaaaaca
    16381 gctccctatc aaaaatgttc tcatctcatc aaagacagtg gcttgaaagg ctcgagctta
    16441 tcctttggtc aatatttgac tttggcgtta aataagatgt ttatgctgaa acacttcaaa
    16501 gaaagtaagc tccattccat tatcattgac tattacctga aatatttaag tcgcttggta
    16561 tccttcaaaa aaaacatttc aatgaaacaa cgactaaatc aactatttaa attggatatg
    16621 tcattgcaaa aaccggaaag attaagagtt ctcgttagcg taacaccaag cttttttacc
    16681 aaagaacgtc ctgatatgag ttttgctaat cttgattaca gctatcctaa tcattttgga
    16741 tgtatggata ggccgatagg aaatcgaacg ctttggattt atttctccaa aaatcaggat
    16801 ggtgaatatc aaatttccat taatgcgcca ttaacaatag aatgtaaaaa taaaatcgga
    16861 ataggtctta gccagtttat tacaactttt gtagagaata ataattgtaa tattgctgaa
    16921 ttaattcaag caatggagga ttcctccatt gcttcctgat tttactgagc ctggttaacc
    16981 gtagtattat tattttttat ttgtttaggg tttgatgatg aaggctgagt tgaaactttg
    17041 ggtaccacaa tatccgttcc aaacttacct tttatcttga cctcacctgg ctgcaataca
    17101 gcagattggc cattggctct acgacaacgc tgggtatggc atttcatctt acaaactccc
    17161 tgaacacatc catagcatcc ccagttatcc caacagcaag cccaattgcc ttcgattgtt
    17221 gaagtaaaag ttccccctgc caactggcag acatctttca tttcagcatt cgatgccccc
    17281 gtattcggtg cagcataaac atgaattgat acaaagctaa aaagaaagag gaaaaacacc
    17341 cctgccatcc ttaaagactt catgattcac ctccttggaa tcgtactatc aggacttgct
    17401 aaaaaaccca atttccttgt taaaaattgc tttcaattta gtcatttagt ttatctaaac
    17461 tccctcatta aaaacatttt ttgccttgac cttggggctt ttcgtaagcc ctaactattt
    17521 aattatggca aaaccccaca attgttcaag ttttaaacaa aaggggtctt gaaacactga
    17581 aaagccccgt cagattactt cattttttct atgccaaata ctgaatagaa tacaaaaaac
    17641 caaagttgcc ataacaatac ccagagcaat gaaaatcggt atcttgaacc aaggtgcgat
    17701 aagcatctta aagcccacaa atactaagat aaaagcaagc ccatatttga gaaaataaaa
    17761 tcgtttgtgc atattcacaa gcagaaaata taaagcgcgt aatcctaaaa ttgcaaatat
    17821 attagaagta aatacgataa aaggatcatt ggttatggca aaaatcgctg gaatgctatc
    17881 cacagcgaaa atcagatcac tgatttcaat aagaaccaac accataaaaa gaggcgttac
    17941 gtagacaagt tgatttttcc tgagaaaaaa tcgctctcca tgtaactcat gggtaattcg
    18001 caaatggctt cgcatccaat gtaatatggg gttttttgcg agctctggtt tcttatcagc
    18061 aaaaatgaac atcttgattc ccgttatcat caggaaaaat ccaaaaatat aaagaatcca
    18121 atgaaattga ttcactatcc atatcccgaa taaaataaga atcaggcgca taacgatggc
    18181 ccctatcaca ccataaatta gaacacgatg ttgatattta gccggtattg caaaatactc
    18241 aaagagaata agaaaaacaa aaatattatc aaacgataaa gacttctcta tcagataacc
    18301 cgtaaaaaat tcaagggctt tttcattggc tactgaaaaa gtaaaattct caacaagata
    18361 ccaccataga agaaaattga aaataatagc cagagtgaac cacaatatgg tccaggataa
    18421 agcttctttt gtggaaacac gatgagcttt tcttccccca aacacaaaca aatcaacaaa
    18481 cagcataatg agcacaaatg ctaaaaaacc taaccacatc caccattcac tgattgaagt
    18541 cattaatttc taccatgtct tttctatggt ttttatatca catcgatatg ttttcattat
    18601 agtcatagta ttagaaatta tgcgcttcaa aaaatattta tattcatttg aaagaaacct
    18661 atggagg
//
LOCUS       DAXLDT010000058        17437 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXLDT010000058 DAXLDT010000000
VERSION     DAXLDT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.058"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            106..996
                     /locus_tag="ACF2SP_001571"
     CDS             106..996
                     /locus_tag="ACF2SP_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFD2359360.1"
                     /translation="MPFSHLLLALLVVFVWGINFIFVKLSLEEFSPLLLCAVRFLLAS
                     VPAVFFIKPPAVPFKIIAGYGLIMFALQFALLFIGLRVGMTPGVASLLMQVQVFFSMF
                     FAIIFLGEQPQVGQIIGALIAFIGIGVVALHFDHNVSLMGFLCILAAAASWGIGNLIT
                     KKIHSVNLIAVIVWSSFVACLPMFILSLVFEGPESFVTAYEHMTWKGILSVLYIVYIS
                     TWVGYGVWNWLISRYPVGMVVPFTLLVPVVGILSSVFILGEPFYLWKLVAGLLVISGL
                     CINLLTTRLFVAKTQQPETA"
     gene            1163..1993
                     /locus_tag="ACF2SP_001572"
     CDS             1163..1993
                     /locus_tag="ACF2SP_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFD2359361.1"
                     /translation="MSDLKNIFLCADDFGLNHGITQGILKMARLQRLSAVSCIVNASS
                     FDVDAPHLLALKNGVRTGLHFNLTEGYFLSHPDKHCFGLKELLIRSHLGILNSRLISD
                     ELNTQLDHFTKVMGKLPDFIDGHQHIHQFPGIRRIIIDIYEQRLNGKSIFIRSTYPSV
                     NMSGYQAKTKILGITGGKTLSSQLKKKNIPHNPYFAGIYDFSARTNYRSLFKQWLNNV
                     PTNTLIMCHPGEGENNDVITVARIRELNYFLSDEFVQDCEEYQVHLTTDSNKLINGII
                     "
     gene            complement(1990..2949)
                     /locus_tag="ACF2SP_001573"
     CDS             complement(1990..2949)
                     /locus_tag="ACF2SP_001573"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD2359362.1"
                     /translation="MSKTTKHKKLISCVVPVFNEEILIAEFIAALDKTLKSITYPYEI
                     LLIDDGSQDNTFAIIQTLRKEYSLRCIRFSRNFGKEKALSAGLDHTRGDAVILMDSDF
                     QHPLELLSEFIAKWEDGYDMVYAVRQNRSDESWLKRVCAKIFYHLTSKIKRINIPANA
                     GDFRLLDRKIVNALQTLPERNRFMKGVYSWVGFKQIAVPFNVHPRKSGSSQWSFYSLL
                     DLAITGITSFTAFPLRMIFLGGMGVAALALVYATWIVLSTLIFGIKTPGWASIVTAIS
                     FFGGLQLFAIGVVGEYIGRVFDEVKHRPHYIIDEESSFDDRQD"
     gene            3095..3772
                     /locus_tag="ACF2SP_001574"
     CDS             3095..3772
                     /locus_tag="ACF2SP_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HFD2359363.1"
                     /translation="MNDSGWFLISTALLLGMRHGFDLDHLATIDAITRTVRYKPSLSR
                     MVGFLFSLGHGLVVIIVSLIIGGGIISASTPEWLNEVGNIVSVTCLLLFGFLTLWNTV
                     HYSSSAIIPTNLRSYLFRKLADNNVHPLMILLIGALFALSFDTISQIVLFSLAASALS
                     GFLFSGLLGFVFMLGMMMSDGLNGFFVASLIRRADGISLLFSRLVGFGIAFFSIGIAI
                     INCCKLL"
     gene            3784..4125
                     /gene="hypA"
                     /locus_tag="ACF2SP_001575"
     CDS             3784..4125
                     /gene="hypA"
                     /locus_tag="ACF2SP_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFD2359364.1"
                     /translation="MHELWLCKRIVEIIKQQATGNKCRMVKKIVLEIGQLVAVDKHAL
                     NFSFKVITQGTIAQNAELSIVEIPGEAICNSCQQIVPMKQYYDECLVCGNHSLTLTKG
                     EELKVKSMVVE"
     gene            4125..4883
                     /gene="hypB"
                     /locus_tag="ACF2SP_001576"
     CDS             4125..4883
                     /gene="hypB"
                     /locus_tag="ACF2SP_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFD2359365.1"
                     /translation="MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQN
                     NEFAAHNRDYFLRHKILVLNLMSSPGSGKTTLLSKTISDLGKEMDIAVIVGDQQTNCD
                     AEMLKSCGGDAWQINTGKVCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHL
                     GEQFRIVILSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNP
                     SIDIITLSAVNGVGLKDWYNWLLHSFRLNAQAST"
     gene            4897..7122
                     /gene="hypF"
                     /locus_tag="ACF2SP_001577"
     CDS             4897..7122
                     /gene="hypF"
                     /locus_tag="ACF2SP_001577"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFD2359366.1"
                     /translation="MELERVQISIQGLVQGVGFRPCVYKLAKQMELTGFVQNNASGVL
                     IEIQGNYLSQFIPKLREQLPPLAAIHNLEFTSLPIVPSERAFEILDSQQGKINTVITP
                     DVCICSECLGELFDPQSRYYRYPFLNCTQCGPRFTITRNLPYDRCQTSMSQFPLCQSC
                     QSDYFNPDSRRYHAQPTACSQCGPQLALSVDKVAQRIRDGEIIALKGLGGYQLICDAR
                     NEGAISQLRLRKSREAKPFALMVVNAKSAEKIAEVSPQARLVLESRERPIVLLRKKKA
                     LLPDGIAPGLNHFGIMLPSTPLHYLLFNSFLGNPDYCQWLDEYHSMVLVVTSANIGSE
                     PLIIDDKKAEQGLISIADKIISHDREIITRTDDSVVRMINDTSVLIRRARGYVPAPLR
                     LPYEIPPTVALGGHLKNTFCITRGEEAFVSQHIGSLNNKATIEYFHESLNHLLKFLAV
                     KPERIAHDWHPDFYTSRLAQKYDIPVYGVQHHHAHLASVVAEYGIQEPVLGLVLDGYG
                     FGSDGEAWGGELLLLEKAEFKRVGHFFPIPQPGGELAAREPWRMAAGILHILGRGDEI
                     SQRFKEQPQSPFLLQLLEKKIHCPLTTSCGRLFDAVSALLGVEFLSGFEGHAAMRLES
                     MVTHPQVLNKGWTINDNVFDMRPTLQFIANTTEQQLGANLFHGTLIAGLSEWVTGICR
                     EKGIHMLLLSGGCFLNQVLAEGLINSLIKSGIKPLLPKLLPPNDGGIALGQAWIVGIK
                     E"
     gene            7132..7359
                     /locus_tag="ACF2SP_001578"
     CDS             7132..7359
                     /locus_tag="ACF2SP_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HFD2359367.1"
                     /translation="MCLALPAQIDQVLEDNKAIVNLGGITKEISTVLLEQVSEGEYVI
                     IHAGFALTKLDEREANKTLSLFAHMLKDGLK"
     gene            7356..8462
                     /gene="hypD"
                     /locus_tag="ACF2SP_001579"
     CDS             7356..8462
                     /gene="hypD"
                     /locus_tag="ACF2SP_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFD2359368.1"
                     /translation="MKYIEEFRNGAYAQAIINEIIATAEPQRHYQFMEFCGGHTHAIH
                     RYGIPSLLPNNVEMIHGPGCPVCVLPIARIDKAIALASMQQVILCSYADMLRVPGSGQ
                     KSLLTTKACGADVRMIYSANDALKIAQENPDKDVVFFAIGFETTTPPTAFVIKQAKKL
                     NITNFSVFCNHVLTPIAMDSLLQANEVALDGFVGPAHVSIVIGSFPYEQVCKKYQKPI
                     VISGFEPLDVLHSILMLIQQLKEGRCDVEIQYTRAVNRKGNLLSQCIMAEVLEIRDRF
                     EWRGLGFIPSSALKIKESFSEFDAEQRFELPESAAQEHKQCLCGEVLRGVKKPTDCKL
                     FAKACLPENPLGSCMVSSEGACAAVYAYGRVNAL"
     gene            8459..9517
                     /gene="hypE"
                     /locus_tag="ACF2SP_001580"
     CDS             8459..9517
                     /gene="hypE"
                     /locus_tag="ACF2SP_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HFD2359369.1"
                     /translation="MSEILITKKLTGPKLNFKEGTINLTHGSGGRAMAQLIEQLFLTA
                     FDNEWLAQKNDQACFIVPSGRMVMATDAHVISPLFFPGGDIGSLSVHGTVNDVAMSGA
                     KPLYLTASFILEEGFPLNDLRKIVYSMADAARQAGVAIIAGDTKVVERGKGDGIFITT
                     TGVGIVPAGIHISGDAAKPGDKIIVSGFIGDHGVAIMAHRNNLEFQTKVQSDTASLHG
                     LVGEMVKAVPELHCLRDPTRGGLATTLNELALQSKVGFVIDESKIPIRTEVASACELL
                     GLDPLYVANEGKLIAICPPEKAEKLLTAMKSHPLGLHAEIIGEVIEDHRCFVQLKTKL
                     GGLRIVDWLAGEQLPRIC"
     gene            9553..10692
                     /locus_tag="ACF2SP_001581"
     CDS             9553..10692
                     /locus_tag="ACF2SP_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFD2359370.1"
                     /translation="MNDKRNCFLPHDKLQHLINSLQNAGYSCVGPQVRDGAIVYDVLT
                     RAEQLPWGKRDHQEPGGYRLEQISERKAFAFANGPQGIKPLLFKPQETVWRVERDEKG
                     KLCFKPHQPDETPVAIIGSRACDLAAMSIQDKVFIESNKPDPRYKRRREQLFVVAVNC
                     TYSSANCFCVSAGTGPAVNNSFDILMTEIEDGFVVEAGSDKGKKIMNQLDLHDAKNAQ
                     CTDAQNNVKQAAALQTKRIPLDNKRDLRDLLFSNLNHTRWDEVAERCLSCGNCTLVCP
                     TCFCHSEVEKPGLDGCTSEHHREWDSCFTTGHTYLNGKIIRDDTKKQYRQWLTHKVGS
                     WFDQFDTSGCVGCGRCATWCPVGIDITEELAAISGESNARKIENE"
     gene            10685..11530
                     /locus_tag="ACF2SP_001582"
     CDS             10685..11530
                     /locus_tag="ACF2SP_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFD2359371.1"
                     /translation="MNEVVYDPYLPKEVEIIQRTQESSSIFTLHLRFTDEDHHKQFQF
                     YPGQFNMLYLYGVGEVAISIVSDPRKKTFLSHTIRAVGRVTKAMQKLQVGDRLGIRGP
                     FGVGWPLQKTIGKDIIVLTGGLGCAPSVSIINYILGRRRHYGKLSILQGVKHSDDFIF
                     RKQYAKWQKSDHTEVYIAADQAGPKWPWGVGYVTDLIDHIMIQPDNSVVMMCGPEMMM
                     NTAVKVFTQKGILENDIYLSMERNMECGIGHCGHCQYGGLFVCKDGPVFAYSQIKELF
                     KESGF"
     gene            11548..12333
                     /locus_tag="ACF2SP_001583"
     CDS             11548..12333
                     /locus_tag="ACF2SP_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HFD2359372.1"
                     /translation="MKPRIAVHKFTSCDGCQLAFLNAGESLLTLSDLVEIVHFAEAGM
                     VDVDVKVDIAFVEGSISTPDEEHRIRKIRENSQYLITIGACATAGGIQALRNFADSRE
                     WMASVYASPDYIKTLTTSTAIAHHVKVDWELWGCPVNSGQILEAIRFLLSNATPKIKR
                     DSECMECKRKGNVCVLVTKKQPCMGPVTQTGCGSLCPTLGRACYACYGPKENANPNSL
                     GEWFLSIGLTKETIARQFLHINNQAPAFNQAGNYFKGIKISNG"
     gene            12326..13618
                     /locus_tag="ACF2SP_001584"
     CDS             12326..13618
                     /locus_tag="ACF2SP_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HFD2359373.1"
                     /translation="MGKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLF
                     EKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGE
                     WLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
                     VGACVGGFYKAPSHPQIKLILEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHP
                     TEYPMNEGRIVSDRGLNITKEEFDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNC
                     FGHLPAPIHLLLQSLNIQFPSRNMCHSIIARAVEMYYCVLESIRILEQYDLPEQSRPE
                     IKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLKHSLIQF
                     GLNQEEEAIRYFSEMIIRNYDPCISCSTHFLNLKMDRI"
     gene            13615..14094
                     /locus_tag="ACF2SP_001585"
     CDS             13615..14094
                     /locus_tag="ACF2SP_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HFD2359374.1"
                     /translation="MNLIKILGIGSPFGDDQVGCLLADCLKTELSKDQYISKLVSIES
                     HDRPGLRLIELMNPAKVVFLIDAVKSGCAIGTIHRLKNKEIYAFQNRLSTHEMGVAQA
                     LQLGHSLNALPEHIILYGIEIDNIAFNAMLSKPVEKAMRAVVIQIKKEIDEALKLMT"
     gene            complement(14154..14873)
                     /locus_tag="ACF2SP_001586"
     CDS             complement(14154..14873)
                     /locus_tag="ACF2SP_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HFD2359375.1"
                     /translation="MVDYHYFKSKLNHFYGRYVGYNQRPVFFDIDQTFPELNHITRHF
                     PDIKKEFENVQANVKEFPRYHDIDPGESAISNTTEKNWNVFMLYLLGYQLKEARLLCP
                     SLCNLLEGIPHLIQAFFSILEPGKSIPLHEGPYIGYLRYHLGIHVPQNNPPQILVNNQ
                     PYTWKEGEAVLFDDSWPHEVRNTSDDYRAVLIIDVLRPMPFLPQLVNQFVTHVIARYF
                     YGRKVMKRAMQYGGEVKPLAL"
     gene            complement(15012..16175)
                     /locus_tag="ACF2SP_001587"
     CDS             complement(15012..16175)
                     /locus_tag="ACF2SP_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359376.1"
                     /translation="MPSGILALGFVSMLTDLSSELIFSLLPVFLVSVLGTNALVIGLI
                     EGFAESIALLIRIFSGMYSDYLGKRKTLAIFGYSFSALSKPLFALATNIGMVFLARFL
                     DRIGKGMRGSPRDALIADIAPDNQYGAAYGLRQSLDTVGAIIGPLLALVLMIIWNNNF
                     QAVFWVAVIPGVLSVLVLILFVQEPVKPASAKPINPIQPKEIKKLSHAYWYVVIIGAI
                     FTLARFSEAFLLLRAYQVGSPITWVPLILVVMYLMYSVSAYPFGKLADTINHSKLLII
                     GLFILITADLILAFSHSLMFIYIGSCLWGLHMGATQGLLAAMVAGTAPDDLRGTAFGF
                     FNFICGIATLIASLLAGLLWDSFGSVFTFSIGALFCVVCLLGIGLMPRIIAKT"
     gene            complement(16362..17294)
                     /locus_tag="ACF2SP_001588"
     CDS             complement(16362..17294)
                     /locus_tag="ACF2SP_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFD2359377.1"
                     /translation="MSKQENIISHNTDSSRLNTVMQQMKERIINEGDKPHVTVETQLK
                     ILQELSQFGFGQFLIKNQGINGYWTHYMLTHPWFGRKTGLNNSREPLSNMECFLLDKA
                     PTMLATQQRFELFLQENQKALVNGATLASIPCGMLGELLYLDFNGINNIRLMGIDYDQ
                     AALEEANALAKERNLDRFITLVCRNAWELNIQEEFDLISSNGLNIYEPDDNKVLDLYQ
                     QFHTALKKGGKLVTSFLTPPPHLSSQCEWMLNKINPEDLLLQRIIFSDILNAKFQCYR
                     SSQQTQKQLALAGFKNIHFLYDEAHLFPTVIAEK"
BASE COUNT         5071 a         3111 c         3803 g         5452 t
ORIGIN      
        1 atgccgaaat tatttgttgt ttatttgctc aattattaag atttcttagc atattctatg
       61 tttataggtt aaaataaaga gacttttata aaagttgacg cattcatgcc tttttcccat
      121 ctcttgttag ctttattggt tgttttcgtt tggggaatta attttatttt cgtcaagctt
      181 agtcttgaag aattttctcc actgcttctt tgcgctgtca gatttctttt agccagtgtc
      241 ccggctgtgt tttttatcaa gcctcctgct gtacctttta aaattattgc tggttatggg
      301 ttaatcatgt ttgccttgca atttgcattg ctttttattg gtttgcgtgt gggtatgact
      361 ccaggagtgg cttctttact catgcaagtg caagtctttt ttagtatgtt ttttgcaata
      421 atatttttag gagaacaacc ccaagtcggg cagataatag gggctttgat tgcatttata
      481 ggcattggtg tagtggcctt acacttcgac cacaatgtgt ctttaatggg gtttttatgt
      541 attcttgctg cagcggcttc ctggggtatt ggcaacctca ttacgaaaaa aattcattct
      601 gttaatctga ttgctgtgat tgtttggagt agttttgttg cctgtttgcc catgtttata
      661 ctttctctgg tttttgaagg acctgaaagc tttgtaactg cctatgaaca tatgacctgg
      721 aaaggtattc tttccgtgct ttatattgtt tatatttcaa cttgggttgg ttatggggta
      781 tggaattggt tgataagccg ttatccagtt ggcatggtag tacctttcac tttattggtt
      841 ccagttgtgg gtattttgag ttccgtcttt attttgggag aaccatttta tctctggaag
      901 ctggttgccg gattgctggt gattagtgga ctttgtatta atttactgac aactcgcctg
      961 tttgttgcaa aaacgcaaca acctgagaca gcctagttat ttcgagctga ttctatatat
     1021 ccttttaaga tcataagtgg agcagggagt taaatttcta tcacccctat aatatggatt
     1081 atcagataat tattttggtt attacaaaat aaggataggg gcagtaatat attacaattt
     1141 tttttggaat tagaagttat taatgagtga tctgaaaaat atattcttgt gcgctgatga
     1201 ttttggcctt aatcatggta ttacccaggg tattttaaaa atggctcgtt tacagcgctt
     1261 atccgcagta agctgtattg tgaatgccag ttcttttgat gtcgatgccc cacacttatt
     1321 agctttgaaa aatggggttc gcactggcct gcattttaat ctcacagaag gttattttct
     1381 atctcatcct gacaagcatt gttttggttt aaaagaatta ctcatcagat cgcatttggg
     1441 tatcttgaat tcaagattaa tctctgatga attgaatact caattggatc atttcacaaa
     1501 agtcatgggg aagttgccgg attttattga tggacatcaa catattcatc aatttcctgg
     1561 tattaggcgt attattattg atatatatga acagcgatta aatggaaaga gtatttttat
     1621 tcgttccact tacccttcgg ttaacatgtc agggtatcaa gcaaaaacaa aaattctggg
     1681 aataactggt ggtaaaactt taagtagcca gttaaagaaa aaaaatatcc ctcataatcc
     1741 ttattttgca gggatttacg atttttctgc cagaacgaac tacagatcct tattcaagca
     1801 atggttaaac aatgttccga caaatacgtt aattatgtgt catcccggtg aaggtgagaa
     1861 taatgacgtt attacagtgg cacgaatcag ggagttgaat tattttttga gcgatgaatt
     1921 tgtacaagat tgcgaggaat atcaggtcca tttaacgaca gactcaaata aattaattaa
     1981 tgggataatc taatcttggc ggtcatcaaa actggactct tcatcgataa tgtagtgagg
     2041 tctgtgtttt acttcatcaa aaacgcggcc tatgtattca ccaactacac cgatagcaaa
     2101 taattgcaat cctccaaaaa aactgattgc agtgactatg gatgcccagc caggggtttt
     2161 aatgccaaag attagtgtgc ttaatacaat ccaagtggca taaacaagcg ccagggcggc
     2221 cacccccatc ccacctagga aaatcattct tagaggaaaa gcagtgaacg atgttatgcc
     2281 agtaattgcc aaatccagta aagaataaaa actccattgt gaactacctg atttacgggg
     2341 gtgaacatta aatggcacgg ctatttgttt aaaaccgacc cagctgtata cacccttcat
     2401 aaaccgatta cgttcgggta gagtttgtag cgcattaaca atttttctat caagcaatct
     2461 aaaatcacct gcgtttgcgg ggatattaat acgttttatt ttagaagtga gatgataaaa
     2521 aattttggcg caaactcgtt ttagccatga ttcgtcagag cgattttgtc ttacggcata
     2581 aaccatgtca taaccatctt cccatttggc gataaattca cttaataatt caagcggatg
     2641 ttggaaatca ctatccatta agattaccgc atctccgcgt gtatgatcca atcctgcact
     2701 gagagctttt tccttgccga aatttctact gaaacgaatg caacgaagag aatattcttt
     2761 tcttaatgtt tgaataattg cgaaagtatt gtcctggctt ccatcatcta tcagtagaat
     2821 ttcataggga taagtgatgc tcttaagagt tttatccaat gcggcgatga attcagcaat
     2881 aagtatttcc tcattaaaaa caggaacaac acaagagatg agttttttat gttttgtagt
     2941 tttagacata atgagtatta atgtacaaga caaaagatag atagcttcat atgttaacct
     3001 actcttggaa gttgagtcca ttgagggtta taaaaacagc gaaagtattg gtatattgtc
     3061 cacagtcttt aacattaata atggcctcat actaatgaac gattcaggtt ggtttttgat
     3121 ttcaactgct cttttgcttg ggatgcgtca tggttttgat ttggatcatc tggcaacgat
     3181 cgatgcgata actcggacag ttcgttataa accttcttta tcgcgcatgg ttggcttttt
     3241 gttttcacta ggccatggtt tggtggttat tattgtgagt ttgatcattg gcgggggtat
     3301 catttcagcg agcactcctg aatggttaaa tgaagtggga aatattgtat cagttacctg
     3361 tttattgtta tttgggtttt taactttgtg gaatacggtt cattattcat cttctgccat
     3421 cataccaacc aacttaagga gttatttatt caggaagctt gctgataaca atgtacatcc
     3481 tcttatgatt cttctcattg gcgcgctgtt tgccttatct tttgatacga ttagtcaaat
     3541 tgtattgttt tcacttgcag ccagtgcttt atcaggtttt ctattttccg ggttattagg
     3601 ttttgttttt atgctgggaa tgatgatgtc ggatggatta aatggatttt ttgtagcaag
     3661 cttgattcgg cgtgctgatg ggatctcatt acttttctca agattagtag gatttggcat
     3721 tgcttttttt agcataggta ttgctataat caattgctgc aaattgcttt aaacaggcta
     3781 aaaatgcatg aattatggtt atgtaagcgt attgtcgaga ttatcaagca acaagccact
     3841 ggcaataaat gccgaatggt aaagaaaata gtgctggaaa tagggcagct agtagcagtc
     3901 gataagcacg cattgaattt tagctttaaa gtgataactc aagggacgat tgcacaaaat
     3961 gcagaattat caattgtaga aatcccaggt gaagcaatat gcaattcttg tcaacaaatt
     4021 gtaccaatga aacagtatta tgatgaatgc ctggtttgcg gcaatcattc cttaacgctg
     4081 acaaaaggtg aagaattaaa agtgaaatca atggtggtgg aataatgtgc gggatatgcg
     4141 gatgttcagt agaaaaaggt caaaccatgg ataatgagca ccatgctact caccatgagc
     4201 attctgtgcc tcatgaacat tcaattgata tcgaacagaa tattttagcg caaaacaatg
     4261 aatttgctgc tcataaccgg gattattttc tcaggcataa aattttggtt ttgaatttga
     4321 tgtcaagtcc tggatccggg aagacaacat tgctttccaa aaccatttca gatcttggca
     4381 aggaaatgga tattgcagtc atagtaggtg atcaacaaac aaattgtgat gccgaaatgc
     4441 taaaatcttg tggaggagat gcctggcaaa ttaatactgg aaaagtatgc catttggatg
     4501 cccatatggt cagtcacgcc atacaagata tttctctaaa aaagcaatcg cttttattta
     4561 tcgaaaatgt gggtaatctc gtttgcccgg cttcatttca tctgggtgag caattcagaa
     4621 tagtgattct ttctgtgaca gaaggagaga ataaaccgct caaataccct gatatgtttc
     4681 attcagcaga tttgctcctg ataaccaaag cagatttatt accccatgtg aattttgatt
     4741 tggatttatg taaggaatat gtacgccgca cgaatccatc catagacatt attaccctgt
     4801 cagcggttaa tggtgttgga ttgaaggatt ggtacaattg gttgttgcat tcttttaggt
     4861 tgaatgccca ggcttctaca taaaaagtgg tagctcatgg aattggaacg ggtacaaatc
     4921 agcattcaag gactagtgca aggggttggt tttagaccgt gcgtttataa gcttgccaaa
     4981 caaatggaat taactggctt tgtacaaaat aatgcatcag gtgttttgat agaaattcaa
     5041 ggcaactatt tatcacaatt tattccgaaa ttaagagagc aattacctcc tctcgcagca
     5101 attcataacc tagagttcac ttcactgcca attgttccga gcgaaagagc ttttgaaatt
     5161 cttgatagcc aacagggtaa aataaatact gtcattactc cagacgtttg catttgttct
     5221 gaatgcctcg gcgaattatt tgaccctcaa agcagatact atcgttatcc tttcttaaat
     5281 tgtactcaat gtggtccaag gttcacaatt accagaaatc tgccatacga tcgttgtcaa
     5341 acatcaatga gccagtttcc cctttgtcaa tcgtgtcaat ccgactattt caatcctgat
     5401 agccgtcgct atcacgcgca acctacagcc tgtagtcaat gtggcccgca actcgcgcta
     5461 tcagtcgaca aggtagctca aagaattcgg gatggagaaa ttattgcttt aaagggtttg
     5521 ggaggatatc aattaatttg tgatgctcga aatgagggtg ctatttcaca attgcggtta
     5581 agaaaaagcc gtgaggccaa accattcgct ttgatggtag ttaatgcgaa aagtgcagaa
     5641 aaaattgccg aagtaagccc ccaggccaga ttggtattgg aaagcagaga aaggccgatt
     5701 gtcttgttaa gaaaaaagaa agcgctttta ccggatggga ttgctccggg cttaaatcat
     5761 ttcggtatca tgctaccttc tactccatta cattatttgc tttttaattc gttccttggc
     5821 aacccggatt attgccaatg gctagatgag tatcattcca tggtattagt ggttaccagt
     5881 gcgaacatag gcagtgaacc gctcattatt gatgataaga aggccgagca aggtttaatt
     5941 tcaatagccg ataaaattat ctctcatgat cgggaaatca ttactcgcac agatgactct
     6001 gttgttcgca tgattaatga tacttcagta ttgattcgtc gggcacgcgg ctatgtgcct
     6061 gctcctttaa gattgcctta tgaaattcct cctactgtgg ccttgggagg acatcttaaa
     6121 aataccttct gtattactcg tggcgaagaa gctttcgttt cacaacatat tggcagttta
     6181 aacaataaag cgacgattga gtattttcat gagtcattga accatttatt aaaatttctt
     6241 gcggtcaaac cagaacgaat cgctcatgat tggcatcctg atttttatac cagtagattg
     6301 gcgcaaaagt atgatatacc tgtttacggg gtgcagcatc atcatgccca cttggcttct
     6361 gttgtagctg aatatggaat tcaggagcct gtattaggat tggttctgga tggctatgga
     6421 tttggttctg atggcgaagc ttgggggggc gaattattat tgctagagaa agcggaattt
     6481 aagcgcgttg gacatttttt ccctattcct cagcccggag gagaacttgc tgcaagagaa
     6541 ccctggcgta tggctgcggg cattttgcat attttgggaa gaggggatga gatttcacaa
     6601 cggttcaaag agcagccaca atcgccattt ttactgcaat tattagaaaa aaagatccat
     6661 tgtccgctga ctaccagttg tgggcgactg ttcgatgcgg tcagcgccct tttaggagtt
     6721 gagttcctgt ctggttttga aggtcatgcg gccatgcggt tggaaagcat ggtgactcat
     6781 ccacaagtct taaacaaagg ctggacgata aacgacaatg tttttgacat gcggcctact
     6841 ttacagttta tagcaaacac gacagagcaa cagttagggg ccaatttgtt tcatggtact
     6901 ttaatagcag gtcttagcga gtgggttaca ggaatttgtc gagaaaaagg gattcacatg
     6961 ctactattga gtggcggctg ttttttaaac caggttcttg cggagggctt aattaattca
     7021 ttaattaaat caggtattaa acccctgttg cccaaattgc ttccaccaaa tgatggtgga
     7081 attgcgctag gtcaagcatg gattgttgga ataaaagaat aagggtggct tatgtgctta
     7141 gcgttgccag cgcaaattga tcaggttttg gaagataaca aagcaatcgt taatttgggt
     7201 gggatcacca aggaaatttc cactgtgctt ttggagcagg tttctgaagg agagtacgtc
     7261 attattcatg caggattcgc cttaacaaaa ctggatgaaa gagaggcaaa caaaacattg
     7321 agtttgtttg cccatatgct aaaggatggc ttgaagtgaa atacatagaa gagtttcgta
     7381 acggtgctta cgcacaagcc attatcaatg aaattatcgc aacagcagag ccgcaaagac
     7441 attatcagtt tatggagttt tgtggaggcc atactcatgc tattcatcgt tatgggattc
     7501 caagtttgct tcccaacaat gttgaaatga ttcatggccc cggttgtcct gtttgcgttt
     7561 tgcctatagc gagaattgat aaggctattg cgcttgcctc aatgcagcaa gtgatcctgt
     7621 gcagttatgc tgacatgttg cgtgtcccgg gaagtgggca aaaaagtttg ttaaccacca
     7681 aagcatgtgg cgctgatgtc aggatgatct attctgcgaa tgatgcatta aaaatcgctc
     7741 aggaaaaccc cgataaagac gttgtgtttt ttgctatagg ctttgaaaca acaacacctc
     7801 ccactgcttt tgtcattaag caagctaaaa aattaaacat cactaatttc agtgtatttt
     7861 gtaaccatgt gctaactccc attgctatgg atagtttgct gcaggccaat gaagttgcct
     7921 tggacggttt tgttggccca gctcatgtca gtatcgtgat tgggagtttt ccttatgagc
     7981 aagtttgtaa aaaataccaa aaacctatcg tgatatcagg gtttgagcca ctggatgtgt
     8041 tgcattctat tctgatgtta attcaacaat tgaaagaggg gcgttgtgat gtggaaattc
     8101 aatacactcg tgcagtgaat cgtaagggta atcttttgtc acaatgcatc atggctgaag
     8161 tcctggaaat acgcgatcgt tttgaatggc gtggtttggg ttttattcca tccagcgcat
     8221 taaagatcaa agagtctttt tctgagtttg atgctgaaca gcgtttcgaa ttaccggaga
     8281 gtgcagcgca ggaacataag caatgtcttt gtggtgaggt attaagagga gtaaaaaaac
     8341 caactgattg caaattattt gccaaggctt gcttgccaga aaatccttta ggatcctgta
     8401 tggtttcatc ggagggagct tgtgcagcag tttatgctta tggacgggtt aatgctttat
     8461 gagtgaaatc ctgataacta aaaaattaac ggggccaaag ctgaatttta aagaaggtac
     8521 tatcaatttg acccatggca gtggtggacg tgctatggct cagctaattg aacaattatt
     8581 tttaaccgca tttgataatg aatggttggc acagaaaaat gatcaggctt gctttattgt
     8641 tccttcagga cgtatggtca tggcaaccga tgctcatgtt atttccccac ttttttttcc
     8701 tggcggggac attggttctt tgtcagtgca tggaactgtt aatgatgtgg ctatgtcagg
     8761 ggcaaaacct ttgtatttga cagccagctt tattcttgaa gaggggtttc ctcttaatga
     8821 tttgcgaaaa atcgtttatt ccatggctga tgcagccaga caagcaggtg ttgccatcat
     8881 tgccggcgat acaaaagttg tcgaacgagg aaaaggcgat ggtattttta ttacgacaac
     8941 cggagtaggg attgtccctg cgggaattca tatttcgggc gatgcagcaa aacctggcga
     9001 caaaatcatt gtgagtggtt ttatagggga ccatggggta gctattatgg cgcaccgcaa
     9061 taacctggaa tttcagacca aagtgcagtc tgacacagcc tctttgcatg ggcttgtcgg
     9121 tgagatggtg aaagcagttc cagaacttca ttgcttgcgt gatccaacgc gtggagggct
     9181 tgcaacgaca ctcaatgaat tggccttaca atccaaagtc gggtttgtta ttgatgaaag
     9241 taaaattccg attcgtactg aggttgccag cgcgtgcgaa ttattaggtc ttgatcctct
     9301 ttatgttgct aatgaaggaa aattaatagc aatttgcccc cctgaaaagg cagagaagct
     9361 attaactgct atgaaatcgc accccttggg tttacatgct gaaatcattg gtgaggtgat
     9421 tgaagatcat cgctgttttg ttcagctgaa aacaaaattg ggcgggttgc gaattgtgga
     9481 ttggctggct ggagagcaat tgcccagaat ctgttaataa gtaataacga tcgacaagaa
     9541 aggacgacca ggatgaacga caagaggaac tgttttttgc ctcatgataa attgcaacat
     9601 ttaattaatt ctttgcaaaa tgctggatac tcctgtgtgg ggcctcaggt gcgtgatggt
     9661 gcgattgttt atgatgtcct gaccagggct gagcaattac cttggggtaa gcgtgatcat
     9721 caagaaccag gtggctatcg attggaacaa atttccgagc gcaaagcttt tgcatttgcc
     9781 aatggccctc agggaataaa gcctctattg ttcaagccac aggaaacagt ttggagggtt
     9841 gagcgtgatg aaaaaggaaa attgtgcttt aaaccacatc agccagatga aacgcctgtt
     9901 gccattatcg gttctcgcgc ttgtgattta gcggcgatga gcattcaaga taaagttttt
     9961 atagaaagta ataagccgga tcctcgttac aaaagaaggc gcgagcaatt atttgttgtt
    10021 gcagtgaatt gtacctattc ctctgcgaat tgtttttgtg tttctgctgg aactggccct
    10081 gcagtgaaca attcgtttga tattcttatg acggaaattg aagatggatt tgttgtggag
    10141 gcaggtagtg ataaaggcaa aaagattatg aatcaactgg atttacatga tgcaaaaaat
    10201 gctcaatgta ctgatgccca aaataatgtg aaacaagccg ctgccttgca aaccaagagg
    10261 attcctctgg acaataaaag ggatttacga gatttgctgt tttcgaattt aaatcacacc
    10321 cgttgggatg aggtggccga acgttgttta tcctgtggta attgtacttt ggtatgccca
    10381 acctgttttt gtcatagtga agttgaaaag ccaggcctgg atggttgtac cagtgaacac
    10441 catagagagt gggactcctg ttttacgaca ggtcatactt atctcaatgg gaaaattatt
    10501 cgtgatgaca caaaaaagca atacagacaa tggttaactc acaaggtagg aagctggttt
    10561 gatcaatttg atacaagcgg ctgtgttgga tgtggacgtt gtgcgacctg gtgtcctgtg
    10621 ggaattgata taaccgaaga gttggcagca atttcaggcg agtccaatgc aaggaaaata
    10681 gaaaatgaat gaagtagtct atgatcctta tttgccaaaa gaagttgaaa ttattcaacg
    10741 tacgcaggaa tcgtcttcaa tttttactct gcacttgcgc tttactgatg aagatcatca
    10801 taagcaattt caattttatc ctggtcaatt caatatgcta tacctttatg gggtcggtga
    10861 agtagccatt tctattgttt cggatcccag aaagaaaact tttctgagtc acactattcg
    10921 agcagtaggg cgggtaacca aagcgatgca aaaattgcag gttggtgatc gtcttggaat
    10981 acgaggtcct tttggagtag gctggccact gcaaaaaacg ataggtaaag acattattgt
    11041 tctaaccggg ggattgggtt gtgccccatc tgtttctatt atcaattata ttttaggaag
    11101 acgacgtcat tacggtaagc tcagtatttt gcagggagta aaacacagtg acgattttat
    11161 ttttagaaag cagtatgcta aatggcaaaa atcagatcat actgaagttt atatagctgc
    11221 agatcaagct ggtcctaaat ggccttgggg tgttggttat gtgactgact tgattgacca
    11281 catcatgata caaccagata actctgtggt gatgatgtgt gggcctgaaa tgatgatgaa
    11341 tacagccgtt aaagtgttta cccaaaaggg gattcttgaa aacgacatct accttagtat
    11401 ggagagaaac atggaatgtg gaattggtca ttgtggtcat tgtcaatacg gtggtttatt
    11461 cgtgtgtaaa gatggtcctg tatttgctta ttctcaaatt aaagagttat tcaaagaatc
    11521 tggattttaa agtgcctgag gaacgctatg aaaccgcgta tcgctgtaca taaatttact
    11581 tcttgtgatg gatgtcaatt ggcattttta aatgcgggtg aatcgttgtt aactttatca
    11641 gacttggttg aaattgtgca ttttgcagag gcaggcatgg tggatgtgga tgtgaaagtg
    11701 gatatcgcat ttgtggaagg cagtatttca actcctgatg aagagcatcg aatacgtaaa
    11761 attcgtgaaa acagtcaata ccttattacc ataggtgctt gcgctacagc gggcggtata
    11821 caagccttac gtaattttgc agattccagg gaatggatgg ccagtgttta tgcgtcaccg
    11881 gattatatta agactttaac tacctcaacg gctattgcgc atcacgttaa agtggattgg
    11941 gagttatggg ggtgtcctgt aaatagcggg cagatattgg aggcgattcg ttttttatta
    12001 tctaacgcga cccccaaaat aaaaagagat tctgaatgta tggagtgcaa acgaaaaggg
    12061 aatgtgtgtg tgctggtgac caaaaaacaa ccttgtatgg ggccagtaac tcaaacaggc
    12121 tgtgggtcgc tttgccccac tttaggcaga gcatgttatg cttgttatgg gccaaaagaa
    12181 aatgctaatc ccaattcttt gggagagtgg tttttatcta tcggattaac caaagaaacg
    12241 attgccaggc agtttttaca tattaataat caggcacctg catttaatca agccggaaat
    12301 tactttaagg gaattaaaat cagcaatggg taaagacatt tcaattaatg ttccaatttt
    12361 ggcgcgggtg gaaggagagg gggctcttga actttgcatt cgggatagca aaattgaaac
    12421 attaaaatta aaaatttatg aacccccaag attatttgag aaatttctgg agggtcgaag
    12481 ttacaatgaa gtgttggatt ttgtagcccg tatttgtggt atatgtcccg ttgcttatca
    12541 aatgagtgct acccaggcta tagagcaatg ttttaaccta agtcccactc cttgggttcg
    12601 cgatatgcgt cgcctgtttt attgtggcga atggctggaa agtcatagct tgcacgtcta
    12661 ttttttggct ttacctgatt ttttaggatt tcaatcagca cctgaaatgg caaggaaata
    12721 tccagaggaa gtgaaatgtg ggttgcggat tcaggctttt ggtaatgatt tgattaaatt
    12781 gttagggggg cgctccgttc atccagtggg agcttgcgta ggcggctttt ataaagcgcc
    12841 ttcccatccg caaatcaaat taatattaga aaaggcaaaa gaacggatct cagattgcga
    12901 agcgttaatt cattggcttg cgagcttatc cttacccgat aattctcatg attttaccag
    12961 tgtttcgctt tatcacccta ctgaatatcc tatgaatgag ggaaggattg tttcagatcg
    13021 tggattaaat ataaccaaag aagaatttga tgcttatttc aaagagcatc atgtggatta
    13081 ctccaatgca ttgcactgtt tattgcaaaa taaaccttat ttagtggggc ctctggcccg
    13141 tattaataat tgttttgggc atttgccagc acccattcat cttcttttac aaagcttgaa
    13201 tattcaattt ccttcgcgga atatgtgtca tagtattatt gctcgagcag tggagatgta
    13261 ctactgtgtt ttggaatcta tccgcattct ggagcaatat gacttgccag agcaatctcg
    13321 tcctgaaatc aaacctcagg caggagaagg agcaggttgt accgaggctc ccagaggtat
    13381 gctgtggcag cattatgttt ttgatgaaac cggtcaagtt ttacaagcac gtattgttcc
    13441 tcccaccagc caaaatcaag cgcgtattga ggaagattta aagcattctt taatacaatt
    13501 tggccttaat caggaggaag aagcgatcag gtattttagt gagatgatca ttcgtaatta
    13561 tgatccatgt atttcatgct ctacacattt tctgaattta aaaatggata gaatatgaat
    13621 cttataaaaa tattaggcat aggctcacct tttggtgatg atcaagtagg atgtttgctt
    13681 gccgattgtc ttaagacaga attgtccaaa gatcaataca ttagtaaact ggtgagcata
    13741 gagtctcacg atcggcctgg tcttcgtttg attgaattga tgaatccagc caaagtcgtt
    13801 tttttaattg atgctgtaaa gtccggatgc gcaataggta caatccatcg cttaaaaaat
    13861 aaagagattt atgcatttca aaacaggtta tcgacccatg aaatgggggt ggctcaagct
    13921 cttcaattag gccatagcct taatgccctg ccagaacata ttattttata tgggattgaa
    13981 attgataaca tcgcttttaa tgcgatgctg tctaaaccag ttgaaaaagc aatgagagca
    14041 gttgttatcc aaataaaaaa agagattgat gaagcactaa agcttatgac ataagtttct
    14101 ttatgcctta ggcggtcttc tcaattgata tcttaaagaa atcgtttctt aatttaaagc
    14161 gccaaaggtt ttacttctcc accatactgc atcgcgcgtt tcattacttt gcgtccataa
    14221 aaatatcgag caatgacgtg ggttacaaat tggttgacca gttgcggcaa gaaaggcata
    14281 gggcgcaaga catcaatgat taatacggca cgataatcat cacttgtatt tcttacttca
    14341 tgtggccagg agtcatcaaa aagtactgct tctccttctt tccaggtata aggttggtta
    14401 ttaactaaaa tttgtggtgg attgttttgg ggaacatgaa ttcctaaatg atatcttaga
    14461 tagccaatat aggggccttc atggagggga atggattttc caggttctaa aatcgagaaa
    14521 aaagcttgta taagatgggg tattccttcc aataaattac aaagcgacgg gcataataaa
    14581 cgtgcttcct tgagttgata accaagaaga tacagcatga aaacattcca gtttttttct
    14641 gtggtgtttg atatggcaga ctctccagga tctatgtcgt ggtagcgtgg aaattctttc
    14701 acattcgctt gtacattttc gaattccttt ttgatatcag gaaaatggcg tgttatgtga
    14761 tttaattctg ggaaagtttg atcaatatca aaaaaaaccg ggcgttgatt atatcctaca
    14821 tagcgcccat aaaagtgatt caatttactc ttgaaataat gatagtctac cattgcttat
    14881 tcctttattt tatttgtgat aataatcagg tgccgaatgt tcgactatcc ttgggaaaat
    14941 cagctatttt aaaatgaagt ttgaagaaat ggaagtttaa aaaatgctca tttttgtgcc
    15001 aaggccgttt ttcaggtttt ggcaatgatt cttggcatta agcctatccc caataagcat
    15061 acaacacaaa ataatgctcc gattgagaac gtaaagactg atccaaaact gtcccatagc
    15121 aatcctgcaa gcaggcttgc aataagtgta gcaatcccac atatgaaatt aaaaaagcca
    15181 aaagctgtac cacgcaaatc atcaggggca gttcctgcaa ccatggctgc taaaagaccc
    15241 tgggttgcac ccatatgcag accccataga caggatccaa tataaataaa cattagtgaa
    15301 tgactaaatg ccaatatcaa atcagctgta attaaaataa ataaccctat aatcaagagc
    15361 ttgctatggt ttatagtatc agccagtttt ccaaaagggt atgcgcttac cgaatacatc
    15421 aggtacatga caaccaggat taagggtacc catgtaatag gtgaaccaac ctgatatgct
    15481 cgtaaaagaa gaaaagcctc gctaaagcgt gccagagtga aaatggcgcc aattataacc
    15541 acataccaat aagcatgact taatttcttt atctctttcg gttgaattgg attaattggt
    15601 tttgcactgg caggttttac tggctcttgc acaaacaaaa taagaaccag tacagacaat
    15661 actcctggta taactgccac ccaaaacaca gcctgaaagt tattgttcca gattatcatt
    15721 aggaccagag ccaaaagagg ccctataata gcacccacag tgtcaagaga ttgccgtaaa
    15781 ccataggctg cgccatattg gttgtctggc gcgatatcag caataagtgc atctctggga
    15841 gaacctctca ttcctttccc tattctatct agaaaacggg caaggaaaac catgccaata
    15901 ttagtagcca aagcaaatag cggtttgctc aacgcgctga aactataacc aaatattgcc
    15961 aatgtttttc ttttacctag ataatcactg tacatcccag agaagattct gattaagagc
    16021 gcaatggatt ctgcaaaacc ctcgattaac ccgatgacta atgcatttgt gcccaatact
    16081 gaaaccaaaa aaacaggcaa taagctaaaa ataagttctg aagaaagatc cgttagcata
    16141 ctgacaaatc caagggcaag gatgcctgat ggcattgaag tcattccatt ttttttcttc
    16201 ctgttgatgc tcatttttcc ctgtaaaaga cgatggttaa tttatcggag gttagttaag
    16261 tccgatatag gctttttatc atgttgccac attctttatt taaggttaaa tactactgtc
    16321 gctgtggtta aaaaatattg ataacaatat cctttttctt gctatttttc agctattacg
    16381 gttgggaaga gatgggcttc atcatataga aaatgaatat tcttgaagcc tgccaaggcg
    16441 agttgcttct gggtttgttg cgatgagcga taacactgga attttgcatt taaaatatcg
    16501 gaaaaaataa ttctctgcag taataaatcc tctggattaa ttttattgag catccattcg
    16561 cattgtgaac tcagatgagg gggaggagtt aaaaaactgg ttactaactt cccgcctttt
    16621 tttaaggccg tatgaaactg ctggtataaa tccagaactt tgttatcgtc tggttcataa
    16681 atatttaacc cattgctgga aatgagatca aattcctctt gaatattaag ctcccaggca
    16741 ttacgacaaa caagcgttat gaacctgtcc aggtttcgtt cttttgccaa ggcattggcc
    16801 tcctctaatg ctgcctggtc ataatctata cccatcagtc ggatgttgtt gatgccattg
    16861 aaatcaagat aaagtaattc ccctaacatc ccacagggga tagaagcaag ggttgcgcca
    16921 ttaaccagag ctttttgatt ttcctgcaaa aataattcga aacgttgttg ggttgctaac
    16981 attgttggtg ctttgtccaa caagaaacat tccatgtttg ataatggctc gcgactattg
    17041 tttaaccccg tctttcttcc aaaccaagga tgagtcaaca tataatgagt ccaataacca
    17101 ttgatgccct gattttttat tagaaattga ccaaacccaa attggcttaa ttcttgaaga
    17161 atcttaagtt gagtttctac cgtgacatgt ggcttatcac cctcgtttat tattctttcc
    17221 ttcatttgct gcattacagt attcagacga gatgaatcgg tgttatgaga aatgatattt
    17281 tcttgttttg acatgcaggc tctctggata atcttttaat tattaattgc tgtgagttta
    17341 agataagaaa atggatttat tttataattc aaactcacaa caattatact taatattctt
    17401 gaagatacgt agatttttta attcatttca ttgtgct
//
LOCUS       DAXLDT010000059        17300 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXLDT010000059 DAXLDT010000000
VERSION     DAXLDT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17300
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.059"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            128..1015
                     /gene="legL1"
                     /locus_tag="ACF2SP_001589"
     CDS             128..1015
                     /gene="legL1"
                     /locus_tag="ACF2SP_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL1"
                     /protein_id="HFD2359378.1"
                     /translation="MLTLSALEKGYDKNLNQLSLSFLNLRDNDIPLLCEFLQNHPAIT
                     SLDLSHNDITANGMKLFVNKTSVSSLNISHNNIGPEGAQWLSEDNHITTLDVSFNDIG
                     DDGIKALAANVKLITLYALYNKITKVGAGYLVQSNLKKIDLCFNSLEDEGVIALASNI
                     NIKELIASACDVSDIGAIELAKNNQLTLLILGKNAITDKATLHFANNTSLSTLHLGSN
                     QITAAGKKILKTNTRITDLDLIGNPIEAHETDELNYSKKSSNSHSVFKFFQKFTFLSC
                     MSPCQETTESDKGLNKKPN"
     gene            1177..2352
                     /gene="ravJ"
                     /locus_tag="ACF2SP_001590"
     CDS             1177..2352
                     /gene="ravJ"
                     /locus_tag="ACF2SP_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavJ"
                     /protein_id="HFD2359379.1"
                     /translation="MAIAPQQIQERLKQEQYQKFVVADIGNFPHCLAQTPEGIASGQR
                     YQKYSTNSLSRTPPFSQWGAPQLLTPKSAQEYIKFAQQRNKKSSFKIDGEAVRVSECS
                     NFAYHSAGVLLDDPQIRTQYDVAVIGSMHSNGRYLHNITLLVPKGSRLPQPPQQLTAE
                     VFPTGTLIVDPWAVGMGHPPEQALAIPKEQFAYNRSLFPATVNYQSALDESLTSTRTG
                     QLTPYTGTPSLTVRQEVRTESQFPDTRRPVSATIPGNFATYHELWRNAFQEIMNDPRH
                     QLHRNDVEYKKIHAIRTVLDDYTKGGDTWWAKFRRIFTFHWNRHHVKVVDDIVKEIDA
                     GNYTTSRALVDRLDNLAISLGSKLNPKGTLKEQISFIKIQEQNPVEEIENLSRPHLA"
     gene            complement(2408..3097)
                     /gene="recO"
                     /locus_tag="ACF2SP_001591"
     CDS             complement(2408..3097)
                     /gene="recO"
                     /locus_tag="ACF2SP_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFD2359380.1"
                     /translation="MTSKSLNAWVIHKQWSGDTSARLKLFTRELGLINCLCKGGRTPK
                     KQSLLQAFIPLWVSIEERYDQYYTRNIESTSSRLDLEGHSLFSGLYINELLYYTLSPD
                     FPDSDLFDAYLFTLNGIALAREREAIEALLRRFEWALLKACGYTFSFLHEARTGELIV
                     PDSHYQFVAGEGFILGGDKKIPGEHLLAIAADNLSESAYLKSAKFIMRQAIDHLLGGR
                     EIKARSLYGPA"
     gene            complement(3087..4022)
                     /gene="era"
                     /locus_tag="ACF2SP_001592"
     CDS             complement(3087..4022)
                     /gene="era"
                     /locus_tag="ACF2SP_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFD2359381.1"
                     /translation="MTWLLECFKNKQESRLMSSYCGYIALVGRPNVGKSTLLNRILQQ
                     KLSITSRKPQTTRHSILGIRTEGEFQFVYVDTPGIHQGNAKAINRMMNKTAISVLRDV
                     DVIAFLVDGTHWKDEDEYVLNLIKQAKVPCILVVNKVDKITDKTQLLPWIEQISQRYQ
                     FAAIIPLSAKTGLQVDELEGKLKTYLPEGPHLFPDDQFTDRSIKFLCAELLREKIFRF
                     CGQELPYSVTVDIESFKDEGNLIRIHALILVDKDNHKRMIIGDKGQKLKEMATNARLD
                     MEKMLDKKVFLQCWCKVKSGWSDDERILKQLGYDQ"
     gene            4237..7362
                     /locus_tag="ACF2SP_001593"
     CDS             4237..7362
                     /locus_tag="ACF2SP_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359382.1"
                     /translation="MPDFETTDGQTQVIIPLRVTPHTHKEMPTITGSIFSHEAGTVFY
                     GNYNMEYLLPGGDRLRLIANHNKKTVQLMLFTHNPELLKILPGDRPSSLVADVINHVN
                     RYKIQAASYKKPLSHEQEEKINLANACIRSLNHLLKKERQLKPHDHAGQEELRRKIIS
                     IIEHCQNDNRMIANNPVVSEGSFGYMLYDAHKTAQHYRFNRVFSVSRQDQMDFTKVTK
                     TQLGKPITPCFVWDSEIHIGHSSSELDDALRVICQQYQLCPAPALNNIPANRFAKLER
                     FISKLWHDGRDWINYLAIITKPGHKTEIQKRSDGVSITKITPYYKLKGLPQRGYNSLA
                     AMVNHLVENSAQQTEVNSIKEAKRLLSHLPNGNWVIVPKKNTVLLRLENKLVHLKYFI
                     HDQLFYPLPDGEDLYILSQISKRHLYLPERISLRFKAFISRIPDFFKGFYKSMSQFII
                     HGLHEEFFNHVHATHAPKQHLSERDQRPKSKKIKRTSLHEALQNQGFLANGQTLEEFI
                     SDQINNSPYVIARANHPPTVHTYENPLHRALGVVRHLAGFFIDTSERNPMIGTLAMAA
                     YIYGAGAVLAPNVLADILKKLHLSGLIYGIEPVQKLAHLMNHGKTSEAISASVTLWQG
                     MVVSGNLDKFFVDAVSVLKEDPAEIAIIVSLALSLGYGLTKAIPSLQHEMGDFPYTNY
                     AALGGKGGAAIYDTIMHPGDDWFLGTCKWFCKNIINIGKLLVAPFFEGFYYGFFDGFI
                     NGWKKSGKLIKQLGKQTLAASVDLILAILTIPLLEVSSLLIHVPFRGITNFFRKLLSV
                     LGNFSAIGKLLIDIAERPSTNNFISEFTLSPLYGFTSPFGHFSDNPVTNIGINGIRLL
                     FLPLLQLIKNILILPLIDTLSLTIRINLSIINPISRLIAYSAGTILYHFGQIWDHSAG
                     VLFSTSAYGLTTLCNHIDSKAGELKQYLLSLIEINRGKVYQWAFHDEDILTHKIVQDN
                     QYYFNDPRRCELIPHSDSHCLLRNLLLNEKSSTNKVKDDPKSQQLHFPGLFHGTTQTP
                     DTMPQEHTLTI"
     gene            7604..9793
                     /gene="lidA"
                     /locus_tag="ACF2SP_001594"
     CDS             7604..9793
                     /gene="lidA"
                     /locus_tag="ACF2SP_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LidA"
                     /protein_id="HFD2359383.1"
                     /translation="MAKDNKSHQVKTSEGSLESVKTKEKEPVVEKMRVEDSKKEDKLS
                     MPTTKKESQPNEPVKPFKTSFEKWIESSLLDPQAKEDRGSTINLGREGLKNASQVKKF
                     LLSPAGKDVIAELGAQMALQRNINLQNQQDRMEHELFKRRLMAALFLWYLSKKSHAAE
                     KAKEIIREYNEKAIKNAEKASKPSPQSTSSTSQADKEIQKMLDEYEQVIKRAQENIKK
                     GEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMETFSKDS
                     EKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSL
                     KDAHLAINKDQEVVEHEGQFYLLQKGQWDAIKNDPAALEKAQKDYSQSKHDLATIKME
                     ALIHKLSLEMEKQLETINDLIMSTDPEKNEEATKLLHKHNGLNLKLANLHDMLAVHRK
                     EKSFFNEKGEEVTSLNDAHYVIGKDQQLFNLGGKFYPIHKEQKILEKDGKFYLLKQGE
                     DWESIKDSPEKQKKAEHDFHKLQYETPMTVKKLVHHNKGLETTIHKERVEETKQQLED
                     NGKEKIEIANNISKLQSTVGVALNQLNQPTLDTESPVLTPSGGSTTSSPKPTPSLASV
                     TTFFKTKIDEMKKNENVKVTFDDLLALSIKIADPKARTYFTGELLKEVPRSGAIPFQT
                     MQRMLQTLEQYGVDTTKPSVTSIRSKTDEVLEQRFNPTPSLSPFKTS"
     gene            9970..10533
                     /locus_tag="ACF2SP_001595"
     CDS             9970..10533
                     /locus_tag="ACF2SP_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HFD2359384.1"
                     /translation="MIIEKILGNQSVIISLDIDNFLFDRLDQIANSDLDLVEINSSDT
                     NLLVKIKTQYPSLKIGAGGIIDTQQLEQCYQAGVHFASSPGFLTSIAQTASVYSMCYF
                     PSVATISEAMAAANIGFKQVRPYPANLTFCNALSKYLPGLNLFPAEIDWNEAELFLNL
                     PAVAAVIIHNPDHKQLNGLARLAQSII"
     gene            complement(10832..11296)
                     /gene="lspA"
                     /locus_tag="ACF2SP_001596"
     CDS             complement(10832..11296)
                     /gene="lspA"
                     /locus_tag="ACF2SP_001596"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFD2359385.1"
                     /translation="MKKWPWLVLSFLVIICDQLSKYWVGVLLTPYKPMPVMPMLNFTL
                     AFNTGAAFSFLSGAGDWHRWFFAGFSFLMSIILLIWLVRTPEQARFQSVGISLILGGA
                     IGNLIDRGIHGYVIDFIDVYYKHHHFATFNVADSAICIGAAILVLDLLIRRE"
     gene            complement(11293..14088)
                     /gene="ileS"
                     /locus_tag="ACF2SP_001597"
     CDS             complement(11293..14088)
                     /gene="ileS"
                     /locus_tag="ACF2SP_001597"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFD2359386.1"
                     /translation="MAEYKDTLNLPNTSFPMKASLSVREPEMLADWQAKGIYQKIRKA
                     RVGSKRFILHDGPPYANGHLHCGHALNKILKDIIIKSKTFSGFDAPFVPGWDCHGLPI
                     ELNVEKKVGKAGSKISPREFRAKCREYAASQIDIQRDEFQRLGVLGDWYNPYVTMDYH
                     YEANIVRALGLMIKNGHLQQGFKPVHWCIDCGSALAEAEVDYEDKTSPSIDVAFSAVN
                     PSEFLNCFETQPAVKPLILPIWTTTPWTLPANEAVCLHPEIDYALIDAGNSYYIVATD
                     LVESVMARYGISHYKTSGSAKGRVFEHFKLQHPFYKRQVPVVLAEHVTTESGTGCVHT
                     APAHGPDDYLVGQSYQLPLINPVMANGCFAENVELFAGISVLKANETILAVLSERNVL
                     LASESIRHSYPHCWRHKSPMIFLATPQWFISMDKSNLRQAIINEIDKVNWVPDWGKAR
                     ISNMVENRPDWCISRQRSWGTPMPLFVHKTTRELHPDTLELIERVAVMIEKSGIDAWF
                     DLDSSELLGDDAKHYDKITDTMDVWLDSGISHYSVLKHNNDLDFPADVYFEGSDQHRG
                     WFNSSLTTAVAMYGVAPYKTVLTHGYTVDAEGKKLSKSKGNYVALDKLVNQHGADILR
                     LWVASTDYRHEVSISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIEAKELLEL
                     DRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDS
                     IARRSCQTAMYHMVKAFTIWLAPILSFTAEEIWQTIPGNNSESIFIEHWYDAWPIIDA
                     VNMEDWEQLHIVRDEVNKALEETRQRGEIGSALAAEVTVYADAKALPKLTRLGEELRF
                     LFITSEAKACPISQSPKGLAVTDCGVSIQVTASAHEKCARCWHRREDVGQNQEHPELC
                     LRCVGNISGYHEERLYI"
     gene            complement(14201..15187)
                     /gene="ribF"
                     /locus_tag="ACF2SP_001598"
     CDS             complement(14201..15187)
                     /gene="ribF"
                     /locus_tag="ACF2SP_001598"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFD2359387.1"
                     /translation="MKLLRGSTHFAIFDKGAVATIGNFDGVHLGHQHLIKALRAKADE
                     MNLPLVILLFEPQPKEYFHREKAPARLSTLREKIDALNLCQVDYIYCIKFDARLAQTS
                     ALYFAQFYLFEALKIRYLLVGQDFRFGKSREGDVNLLKTLGANYSCEVTVQSDFLIEN
                     EKISSTRIREALQQGNLRFAAKLLGRPYSMCGRVIKGDGRGRQWGIPTANLGMHRLSI
                     PLQGVYAVQVHINSNVFYGVANIGKRPTVAGTKNILEVHLFDFHGSLYGQLVQVIFLH
                     KLRDEVKFSSVEALITQINSDIILAKAYLRQNWLDSVIDSSNDVANGINNTV"
     gene            complement(15311..15742)
                     /locus_tag="ACF2SP_001599"
     CDS             complement(15311..15742)
                     /locus_tag="ACF2SP_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD2359388.1"
                     /translation="MYKRVLFATDFDEVGVRAAEKAKKIADENGADLILVHVVEPIPA
                     YAYPGFAGFAEVEMSIREQAEKELNELAEKLGVDEKHRFIEYGSTKNEILRVAKEHKI
                     DLIVTGSHGKHGLSLLLGSTANAILHGAECDVLIVRPKIHH"
     gene            complement(15851..17299)
                     /locus_tag="ACF2SP_001600"
     CDS             complement(15851..17299)
                     /locus_tag="ACF2SP_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFD2359389.1"
                     /translation="MDGKEPLDEGVANQPRTLFKPGAWLAKVDFINHLVLFNNVLITI
                     LSEKEGGKTCFNSLLKSHLNQQIKSVSFKAEPPFDSQDIINEFARQLHLNNKDNNIGS
                     LAAQINERKTHVLLIIDDAHHLPENFIKEVMIIIKNQEDFGFFHLCLISDYSIVATLN
                     NLAVEPFNNLIHTIELGALSETETRTYVLQRAMASHLINRPLTDAQFKQFYHLTKGNL
                     SKINSNLELFLSKCANEKQNNKKAILKRISLVASFAIVAGLSYFYFDNVYKAPDNTPK
                     LPDLASVATLPPINDLIKTPTQQAVPKQDSVSYIASWEDSSTRLLVHYGLPKKQILDD
                     FMDEEDTLNTVAIVDKVVVIPKLKAKNISEQVGQAVVSQSKLPPIPHSSQTKLASEQP
                     GIKKINSKLFTIQLAASHKVEDINRFKNQNKWLAQAKIRHFTNAKGSWYILTIGEYES
                     RNQALLNIKKLPGELAKLSPWIRSVEGLSNIG"
BASE COUNT         5627 a         3589 c         2993 g         5091 t
ORIGIN      
        1 gatgcctgaa taataggcac tgatcacatt tccctgaatc atcataaact aaacaaaaat
       61 cttgatatca ttttcttgtt taaataaaat attaaacaat tttaattata attgaacacg
      121 gtgttagatg ttaacattat cggctttgga aaaaggctat gataaaaatc ttaatcaact
      181 ctcattatct tttttaaatc tcagagataa tgatatacca ctgctatgtg agtttcttca
      241 aaatcatcct gccataacca gtcttgactt gtcacataat gatattacag ccaatggcat
      301 gaagcttttt gttaataaaa catcggtttc atcccttaat attagccata ataatattgg
      361 tccggagggt gctcaatggc tatcagaaga taaccacatt actactttag atgtcagttt
      421 taacgacatt ggtgacgatg gaattaaagc tctagccgct aacgtcaaac tgataaccct
      481 ttatgcactt tataataaga taaccaaagt aggggcaggc tatcttgttc aatccaatct
      541 taagaaaatc gatctctgct tcaatagtct ggaagatgag ggggttatag cattagcaag
      601 taatatcaat ataaaagagc tgattgcaag cgcttgtgat gtctctgata ttggagctat
      661 tgaattagct aaaaacaatc aactgacttt acttattttg gggaaaaacg cgataactga
      721 taaagcaaca ctccattttg ctaataacac ttctttgagt acccttcatc tgggtagtaa
      781 tcagattacg gcagcaggta aaaaaatact taaaaccaat accaggataa cggaccttga
      841 tttaatcggc aatccaattg aagcgcatga aacagatgaa ctaaattatt ctaaaaaatc
      901 gtcaaactcc catagtgtat ttaagttttt tcaaaaattt acttttctca gttgtatgtc
      961 accttgtcaa gaaaccacag aaagcgataa aggcttaaat aaaaaaccga attaattatg
     1021 aaatcacgat tatcttaatc aagattcagt aaaaacaata acctaagagg taaattttta
     1081 ttcgcaccta taaaaagaaa agcactatat ttttaaagaa atcatggtta agaaataacc
     1141 gccacaacaa actcaaatca gttatcggag taatttatgg ctattgcccc ccaacagata
     1201 caagagagac tgaagcaaga acaatatcaa aaatttgttg ttgctgatat tgggaatttt
     1261 ccacattgcc ttgctcagac tcctgaaggc atagctagcg gacaaagata ccaaaaatac
     1321 agtacaaact ccttatccag aacaccacct tttagtcaat ggggagctcc ccagctctta
     1381 acccctaaat cggctcagga atatattaaa tttgcccagc aaagaaataa aaaatcaagt
     1441 tttaaaattg atggtgaggc agttcgtgtc tctgaatgca gcaattttgc ttaccactct
     1501 gcaggagtat tgttagatga tccacaaata agaactcagt atgatgttgc cgttatagga
     1561 agtatgcaca gtaacggccg gtatctacat aatattacct tactcgttcc taaaggaagc
     1621 agactccctc aacccccaca gcaattaacc gctgaagttt ttccaactgg tacgctaatc
     1681 gttgatccct gggccgttgg catgggacac cctcctgaac aagcattggc gattcccaaa
     1741 gaacaatttg cttataatcg cagccttttt cccgccacag tgaattatca gtcagctctt
     1801 gacgagagtc ttacttcaac cagaacaggc cagctcactc cctatactgg tacaccttca
     1861 ctaactgtaa ggcaagaagt acgcacagaa agccagttcc ctgatacaag aaggcctgtt
     1921 tctgcaacga tccccggaaa ttttgctact tatcatgaat tatggcgaaa tgcttttcaa
     1981 gaaattatga atgatcctcg ccatcagctc cacagaaatg atgtagaata caaaaagata
     2041 catgccatta gaactgttct tgatgattat actaaagggg gcgacacctg gtgggccaaa
     2101 ttcagacgaa ttttcacttt tcattggaat agacaccatg tcaaagtagt tgatgacatt
     2161 gtcaaggaga ttgatgctgg taattacact acctcacgag ctttggttga tagattagat
     2221 aatcttgcta tttcattggg aagtaaactc aatcctaaag gtactttaaa agagcaaatt
     2281 agttttatta aaattcagga gcaaaatcct gtggaagaaa tcgaaaatct atccagacct
     2341 cacctggcat aataaaccat ggacctgatg tgatagcata gaagcacttg gcgatcatac
     2401 cactatctca ggccggtcca tacaaggacc gggccttgat ttcccgtccc cccaaaagat
     2461 gatcgattgc ctgacgcata atgaatttcg ctgattttaa ataagcggac tcgcttaaat
     2521 tatctgccgc tatagcaagt aagtgctcac caggtatttt tttatcacca cctaaaataa
     2581 agccctcccc agctacaaat tggtaatgag aatcgggaac aattaactct cccgttcgcg
     2641 cctcatgaag aaatgaaaat gtgtatccac aagccttaag caaagcccat tcaaagcgac
     2701 gcagcaaagc ttctatcgcc tctctttctc tagctaatgc tatgccattt aaggtgaaca
     2761 agtaagcatc aaataaatcc gagtcaggga aatccgggct cagcgtataa tagagtaact
     2821 cattgatata caggcctgaa aaaagagaat gtccctcaag atccagtctg gaagaagtgc
     2881 tttcaatgtt tcgtgtgtaa tattgatcat aacgctcttc aatactaacc cacagaggaa
     2941 taaaagcttg aagtaatgac tgtttcttgg gagttcgccc ccccttgcac aagcaattaa
     3001 ttaatcccag ttccctagta aaaagcttta gtctggcact ggtatctcca gaccattgct
     3061 tatgaattac ccaagcattc agcgatttac tggtcatatc ccaattgctt cagtattcgc
     3121 tcatcatctg accagcctga tttcacttta caccaacatt gtaaaaaaac ctttttatct
     3181 agcatttttt ccatgtccaa acgggcatta gttgccattt cttttaattt ttggccttta
     3241 tcaccaatga tcatgcgctt gtgattatcc ttgtctacta aaatcaaggc atgaatacga
     3301 attaaatttc cctcatcctt aaatgattcg atatctactg ttactgaata aggcaattct
     3361 tgtccacaaa agcggaaaat tttttcacgg agcaattcgg cacataaaaa ttttatagac
     3421 cgatcggtga attgatcatc aggaaacaaa tgtggtcctt ctggcaaata agtctttaat
     3481 ttgccttcca attcatccac ctgcaaacca gttttcgccg ataaaggaat aatggctgca
     3541 aattgatatc gctgacttat ttgctctatc caaggcaaaa gttgtgtttt atcagttatc
     3601 ttgtcaactt tgttaactac caggatgcaa ggaactttag cttgtttaat taaattcaat
     3661 acatactcat cttcgtcttt ccaatgcgtt ccatcgacta gaaaggcaat cacatccacg
     3721 tcacgtagta cactaatcgc tgttttattc atcatgcgat taattgcttt cgcattaccc
     3781 tgatgaatac caggagtatc tacataaaca aactgaaatt caccttctgt gcgaattcct
     3841 aaaatactat ggcgcgtggt ttgtggtttt cttgaggtaa tacttaattt ttgctgtaaa
     3901 atacgattca ataaggttga tttgccaacg tttggcctgc caactaaagc aatataacca
     3961 caataactac tcattaatct tgattcctgt ttatttttaa aacattctag cagccatgtc
     4021 ataaaacttc cagagatacc aaatttttca ttaaaataga cttgctattt ctgctccaat
     4081 tcgagtaccc tgtgcattaa tggcattaaa ttaaacgatt ttgtattatt ttagcttaaa
     4141 tgggctttac ttttcttcaa aaccaactta agcgataaaa aatcactaaa ttaatgacag
     4201 tatcctttta ccatatatta aaccaaataa atctttatgc ctgattttga aactactgat
     4261 ggccaaactc aagttattat tccactcaga gttactccgc acacacacaa agagatgcca
     4321 actatcaccg gaagtatttt cagtcacgaa gccggtacag tattttatgg taattataat
     4381 atggaatatc tccttcctgg gggagacaga ctacgcttaa tagctaatca caacaagaaa
     4441 acagttcagc tgatgctctt cactcataac cctgaactgt taaaaattct tccgggagac
     4501 agaccatcct cgctagttgc tgatgttata aatcacgtca atcgctataa aattcaagct
     4561 gcgagctata aaaaacccct aagccatgag caggaagaaa aaattaacct ggctaatgct
     4621 tgtatccgtt ccttaaacca tctattgaaa aaagaaagac aattaaaacc tcatgatcat
     4681 gccggacagg aggagttaag aagaaaaatc atttccatta ttgaacattg ccagaacgat
     4741 aatcgaatga tagccaataa tcctgtggtt tcagaaggaa gttttggtta tatgctatat
     4801 gatgcgcaca aaacagcaca gcattatcgg tttaaccgag ttttttctgt ttctcgccag
     4861 gatcaaatgg actttaccaa agtgactaaa acacaacttg gaaaaccaat tactccttgt
     4921 tttgtttggg atagtgaaat ccatattggg cacagtagca gtgagcttga cgatgcgctc
     4981 agggtaattt gtcagcaata ccaactctgc cctgcgccag cattaaacaa catcccggcc
     5041 aatcgctttg caaaactgga acgattcata agtaaacttt ggcatgacgg tcgcgattgg
     5101 attaattatc tggcaatcat aacaaaacca ggtcataaaa ctgaaataca aaaacgctct
     5161 gatggagtat ctatcaccaa aataacgcct tactacaaac tcaaaggact gcctcaaaga
     5221 ggttataata gtctggctgc aatggtaaat catctagtgg aaaactcagc ccaacagact
     5281 gaggtaaaca gtataaaaga ggcgaaaaga ttactaagcc acttacctaa tggtaattgg
     5341 gttattgtgc caaaaaaaaa tactgttctg ttaagactcg aaaataaact ggttcatctt
     5401 aaatatttta ttcatgatca actcttttat cctttgccgg atggagagga tttatatatt
     5461 ttaagccaaa tcagcaaaag acatctttac ttgcctgaaa gaatcagcct ccgtttcaaa
     5521 gcatttattt cccgtatacc tgattttttt aaaggttttt acaaaagcat gagccaattc
     5581 atcatacatg gcctgcatga agagtttttt aatcatgtgc atgcaactca tgctcctaaa
     5641 caacatcttt cagaaaggga tcagcgtcca aaatccaaaa aaattaaaag aacctctcta
     5701 catgaggcgc tccaaaatca gggatttctc gcgaatggtc aaactcttga agagtttatt
     5761 agtgatcaga tcaataacag cccctatgtc attgcaagag cgaatcatcc ccctactgtg
     5821 cacacctatg aaaatccttt gcacagagct ttgggcgtag tacgccattt agccggattt
     5881 tttatagaca ctagcgaacg taaccctatg attggaactc ttgccatggc agcttatatc
     5941 tatggcgcag gagctgtact cgcacccaat gttctcgctg acattttaaa aaagctgcac
     6001 ttaagtggct tgatttatgg aattgagcct gttcaaaagt tagctcactt gatgaatcat
     6061 ggcaagactt cagaagctat ttctgcctct gtaactctct ggcaaggtat ggtagtgagc
     6121 ggcaatttgg ataagttctt tgtcgatgct gtctctgtcc tgaaagaaga cccggcagaa
     6181 attgctatta ttgtttcttt agcgcttagc cttggttatg gtttaacaaa agcgattcct
     6241 tcactacaac atgaaatggg tgactttcct tataccaatt atgctgctct tggaggtaaa
     6301 ggaggtgccg ctatctatga taccattatg cacccgggtg atgactggtt ccttgggact
     6361 tgcaagtggt tttgcaagaa tattataaat attggaaaat tattggttgc accttttttt
     6421 gaaggttttt actatggttt ttttgatgga tttataaatg gctggaaaaa aagtggcaaa
     6481 ttgataaaac aactgggtaa acaaacttta gccgcaagtg ttgatcttat tttagctatt
     6541 ttaaccatcc ccttacttga ggttagttcg cttttaatac acgtcccttt tagaggaatt
     6601 accaattttt tccgcaaatt actgtctgta ttaggtaact tcagtgcgat tgggaaatta
     6661 ctcatcgata tagcagagcg tccatccaca aataacttca tatctgaatt tacgctttcc
     6721 cctctttatg gatttacttc cccttttggc catttttcag ataatccagt cacaaacata
     6781 ggcattaatg gcataaggct gcttttttta cccctcctgc agctgattaa aaatattttg
     6841 attctccctc tgatagacac cctttcacta acaataagaa taaatttatc tattattaac
     6901 cctatttccc gacttattgc ttattctgcc ggcactatcc tttatcactt cggacagatt
     6961 tgggatcatt cagcaggagt tttgttctca acaagcgcgt atgggctgac tacattatgt
     7021 aaccacatag atagcaaagc gggtgaatta aagcaatatt tgctttcctt aatagaaata
     7081 aatcgtggaa aagtatatca atgggcattc catgatgagg atatactgac acataaaata
     7141 gtgcaggata atcaatacta tttcaatgat cccagacgtt gcgaattaat tcctcactca
     7201 gacagtcact gcttgttacg caatttatta ttgaacgaaa agagctctac caataaagtc
     7261 aaagatgatc cgaaatccca acaactgcat tttccaggcc tgtttcatgg taccacacaa
     7321 acaccagaca caatgcctca agaacataca ctgactatct aaacacttac aaatctaatc
     7381 gcattgattt catggttttt atttgcaaat tatgctgctt gcttctaaga tgaaatacta
     7441 atattcagca aaaaacggct ccaatcatgc atgagttgca aactcaagat cgcacgatag
     7501 taaatggcat gatcctgtta aaacatgaat aaactgatgc tttattggtc aacattgtgt
     7561 ataatataag ataactgggg gcgattgatt aaggatccta tttatggcaa aagataacaa
     7621 atcacatcaa gttaaaacat cagaagggag tcttgaatcc gtaaaaacca aggaaaagga
     7681 acctgttgta gaaaaaatgc gtgttgagga tagtaaaaaa gaagataagc tatcaatgcc
     7741 caccactaaa aaggaatctc aacccaatga gccagtcaag ccctttaaaa cttcttttga
     7801 gaaatggata gaatccagct tgcttgatcc acaggctaag gaagaccgag gctccaccat
     7861 taatctgggc cgagaagggt taaaaaacgc atcccaggtc aaaaaatttc ttttatctcc
     7921 agcaggtaaa gacgtaatag ctgagttagg tgcccaaatg gccctgcaaa gaaacatcaa
     7981 cctacaaaat caacaagaca gaatggagca tgagctcttt aaacgccgat taatggccgc
     8041 tttgtttttg tggtatttat ccaaaaaatc ccatgctgcg gaaaaagcca aggaaattat
     8101 tcgggaatac aatgaaaaag ccataaaaaa tgctgaaaaa gcaagcaagc caagcccaca
     8161 gtccacttca tccacttcac aagctgataa agaaattcaa aaaatgctgg atgagtatga
     8221 acaagttatt aaacgtgctc aagagaatat taagaaaggc gaagaattag aaaaaaaact
     8281 ggataagctg gaacgtcaag gcaaagatct tgaagacaag tacaaaacct atgaagaaaa
     8341 tttggaagga tttgaaaaac ttcttactga ttctgaagag ttatcactat ccgaaattaa
     8401 tgaaaaaatg gaaaccttca gcaaagacag tgagaaactc acgcaactga tggaaaagca
     8461 taaaggcgat gagaaaaccg ttcaatcctt acagcgtgag catcatgaca taaaagcaaa
     8521 actggctaac ctgcaagttt tgcatgacgc tcatacaggt aaaaaatcct atgttaacga
     8581 aaaaggaaac ccggtcagct cattaaaaga cgcgcacctg gccattaata aagaccaaga
     8641 agtagtagaa catgaaggac aattctatct cctgcaaaaa ggtcaatggg atgccattaa
     8701 gaatgatcct gcagcattag aaaaagccca aaaagactat agccaatcta aacatgatct
     8761 tgcaaccatc aagatggaag ctctaattca taagcttagt ttggaaatgg aaaaacaatt
     8821 ggaaacaata aatgatctta ttatgagcac agatccagaa aagaatgagg aagccaccaa
     8881 attactgcat aaacacaatg gattaaattt aaaattggca aatctacatg acatgcttgc
     8941 agtacataga aaagaaaaat ccttttttaa tgagaaagga gaggaagtga catcattaaa
     9001 tgatgctcat tacgtgatag gaaaagatca acaactgttt aatttaggcg gtaaattcta
     9061 tcctatccat aaagaacaaa aaatacttga aaaagacggc aaattttatc ttttaaaaca
     9121 aggtgaagac tgggaatcta tcaaagatag tccggaaaaa caaaaaaaag ctgagcatga
     9181 ttttcataaa cttcagtatg aaacaccaat gacagtcaag aaattagtgc atcacaataa
     9241 aggtttagaa acaaccattc ataaagaacg agttgaagaa acgaaacaac aattagaaga
     9301 caatggaaaa gaaaaaatag aaatagccaa taacataagt aaacttcaat ctacagttgg
     9361 tgtggcttta aatcaactca accaaccaac tttagatact gaatcacctg tcttaacacc
     9421 ctccggagga agtacaacca gttcaccgaa accaaccccg agcttggcgt cagtgactac
     9481 ttttttcaag acgaagatag atgaaatgaa aaaaaatgaa aatgttaaag ttacctttga
     9541 tgatctgctg gcgctcagta ttaaaattgc cgatccaaaa gcacgtactt attttactgg
     9601 tgaactactt aaggaagtgc cacgatctgg agcaataccg tttcaaacta tgcaacgtat
     9661 gctgcaaact cttgagcaat atggggtaga tactacaaaa cccagtgtta cttctataag
     9721 aagcaaaaca gacgaagtcc ttgagcaacg atttaaccct actccgtctt tatctccatt
     9781 taagacatca taagatagaa ttatctttca tctccctacc ctggattatt gtctgagaat
     9841 cataattcag ggtgctctac cctccacctc atcatcaaac aaattgttgt ttttcttatc
     9901 aatacccttg ccaacaatca gcaagtcttc taacattgaa agattattgc agaattttcg
     9961 gaataaaaca tgattattga aaaaatctta ggtaatcaat ctgtgatcat atcattggat
    10021 atcgataatt ttttatttga cagactcgat caaattgcaa attccgattt ggatttagta
    10081 gagatcaact cgtccgatac caatttattg gtaaagatta aaacccaata cccatctcta
    10141 aaaattggag ccggcggcat aattgatact caacaattag agcaatgcta ccaggcgggt
    10201 gtacattttg cctcgagccc cggcttttta acttctatag cccaaactgc aagtgtttat
    10261 tcgatgtgct attttccaag tgtagctacc atatcagaag ctatggccgc agcaaatatt
    10321 ggatttaaac aagtgcgacc ctaccctgcc aatctgacat tttgtaatgc tctaagcaaa
    10381 tatttaccag gattaaatct gttcccagct gaaattgact ggaatgaagc tgagcttttc
    10441 ctaaacttgc cagctgtagc agcagtaatt attcataacc ctgaccataa gcaactgaat
    10501 ggattagcca ggttagcaca atccattatc tgaaaataaa tcgattctaa ttggtaagca
    10561 ttgttttcca ttaatgcatg acaagctttg gtatagtatt ttgacttaga aaagggttaa
    10621 gatactaaac cacttcggca ctattatttc tgtaagagat cagcctcctg ccgagaaagt
    10681 cttttttttt gctcgtcttg aacgagaata gccatctatt ctgtgctttt tcaggattat
    10741 ttgcatctaa ccagaacaca aaccctactg ttataggata gtcaaacatg aggacactaa
    10801 cttatgacat tatttcctag gcagtttgag tctattccct acgaatcaat aaatccagaa
    10861 ctaaaattgc ggctcctata catatagcgc tatcggccac gttgaacgtg gcaaaatggt
    10921 ggtgtttata ataaacgtca ataaaatcaa tcacataacc atgaatacct ctgtcaatca
    10981 agttgcctat agcgcctcct agaataagac ttatcccaac agattgaaat cgtgcctgtt
    11041 ctggtgtccg cactaaccaa atcagcaaaa taatactcat taaaaaacta aagcctgcaa
    11101 aaaaccacct atgccaatca cctgcaccgc ttaaaaaact aaaagcagcg cctgtgttaa
    11161 aagctaaagt gaaattcaac ataggcataa ctggcatggg tttatatgga gttaataaca
    11221 ctccaaccca atacttactt aactgatcgc agataattac taaaaaacta agtacaagcc
    11281 aaggccattt tttcatatgt aaagcctctc ttcatgatat ccgctgatat ttcctacaca
    11341 acgcaagcac agttcaggat gctcttgatt ttgaccaaca tcttcccgtc tatgccaaca
    11401 tcgagcacac ttttcgtgag cgctggctgt aacctgtatt gataccccgc aatcagtaac
    11461 agctaatcct ttgggggatt gactaattgg acaagctttc gcttctgatg taatgaataa
    11521 aaagcgtaac tcctcgccta aacgcgttaa ttttggtaat gccttggcat cagcataaac
    11581 agttacctca gccgccaatg cagaaccaat ttcacctctt tgcctggttt cctctaaagc
    11641 cttgtttact tcatcacgaa cgatatgcag ctgttcccaa tcctccatgt tcactgcgtc
    11701 aataattggc caggcatcat accaatgctc aataaaaata gattcgctat tattcccagg
    11761 tattgtttgc catatttcct cagctgtaaa agataagata ggcgctaacc agatagtgaa
    11821 tgctttgacc atatgataca tcgctgtttg gcaagatcgt ctcgctatac tgtcttttgc
    11881 tgtggtgtat tgcctgtcct ttatcaaatc caaataaaaa ctacccatat ccactgcgca
    11941 gaagttgtgg attttctggt aaatcaaatg gaagtggtaa ttttcatagg cagtaattat
    12001 ttcttcttgt aaaagttggc aacgctttag agcccacctg tccagttcta ataactcctt
    12061 ggcctcaata caatcactgg ctggattaaa gtcaaataaa ttagctaata aaaatcgcgc
    12121 tgtattacga attcttcgat aagcatccgc gttacgctta atgatttcct cagaaatact
    12181 tacttcatga cgataatcag ttgacgctac ccataatctt aaaatatcgg caccatgctg
    12241 attcactaat ttatctaaag caacataatt tcctttagac tttgatagtt tttttccctc
    12301 ggcatcaaca gtatacccat gagtcaatac ggttttgtag ggagcaactc catacattgc
    12361 aactgcagtt gttaatgagg agttaaacca acctctgtgc tggtcggatc cttcaaaata
    12421 gacatcagcc ggaaaatcta aatcattatt atgttttaaa acgctataat gtgatattcc
    12481 ggaatctagc caaacgtcca tggtgtctgt gattttatca tagtgtttgg catcatcgcc
    12541 aagcaactcg gagctatcca gatcaaacca ggcgtctatg cctgatttct caatcatcac
    12601 agcaaccctc tctattaact ccaaagtgtc aggatgtaat tctctggtgg ttttatgcac
    12661 aaacaatggc atgggtgttc cccaagatct ctgccttgat atacaccagt caggcctatt
    12721 ttcaaccata ttgctgatgc gcgccttacc ccagtcagga acccagttaa ccttatctat
    12781 ctcgttaatt attgcttgtc gcaaattgga tttatccatt gagataaacc actggggcgt
    12841 agccagaaat atcattggcg atttatgacg ccaacaatgc ggataactgt gcctgataga
    12901 ttcactagct aacaaaacat tccgctccga caaaactgct agaatagtct cattcgcttt
    12961 caatacagaa atgcctgcaa ataactctac attctcagca aaacatccgt tggccatgac
    13021 aggatttatt aatggcaact gataggactg acctaccaaa tagtcatcag ggccatgagc
    13081 aggtgcagta tgcacacaac ccgtaccaga ctctgttgtc acatgctcag caagaactac
    13141 gggaacctga cgcttataaa atgggtgctg cagtttaaaa tgctcaaaga ctcgcccctt
    13201 agcggagccc gatgttttgt aatggctgat tccataacgc gccataaccg actcaaccaa
    13261 atcagtagcc acgatataat aagaattacc tgcatcaatc aatgcataat caatttcagg
    13321 atgcaaacat accgcttcgt tagcaggcaa tgtccatggc gttgtagtcc aaataggcag
    13381 aattaatggt ttgaccgctg gctgagtttc aaaacaatta aggaactcgg atggatttac
    13441 tgctgagaac gccacatcaa tggatggaga agttttatcc tcataatcca cctctgcttc
    13501 agccaacgca gatccgcaat ctatacacca atgaacgggt ttaaagcctt gttgtaaatg
    13561 accattttta atcattaaac ctaaagctcg tactatattg gcttcataat gataatccat
    13621 agtcacataa gggttatacc agtctcccaa gactcccaat cgttgaaact catctctttg
    13681 tatatcaatt tggcttgctg cgtattctcg acatttggca cgaaattctc ttggactaat
    13741 cttgctccct gctttaccta cttttttttc tacattcaat tctataggca agccatgaca
    13801 atcccaaccg gggacaaaag gggcatcaaa accactaaag gtttttgatt taataataat
    13861 atctttgaga attttattga gcgcatgccc acagtggaga tgcccgttgg catagggcgg
    13921 accatcatgc aaaataaagc gcttacttcc aacacgagct ttacgaattt tctgataaat
    13981 tccctttgct tgccaatccg ccaacatttc aggttctcgc actgacaaac ttgctttcat
    14041 tggaaatgaa gtgtttggaa ggtttaaagt atctttatat tctgccatag gtgcctactc
    14101 tgccaaatca ttaagttttt gataatttta aatgcccttg ttctgtttcg tctgcacagc
    14161 ccaaagggag cttacaaaaa atacccgaat taagggtaaa ttaaacggta ttattgattc
    14221 cattcgcaac atcattcgat gaatcaatca cactatcaag ccaattctgt cttaaatagg
    14281 cctttgccaa tatgatgtca ctattaatct gcgtaattaa cgcttccaca cttgagaatt
    14341 taacttcatc tcgaagtttg tgcaagaaaa ttacctgcac tagctgacca tacagagacc
    14401 cgtgaaaatc aaataaatgg acctccaaaa tatttttggt tccagctact gttggccgct
    14461 ttccaatatt tgcaacccca taaaagacat ttgaattaat atggacttgt acagcatata
    14521 caccttgcaa agggatcgat aaacgatgca tgcctaaatt ggctgtcggt atcccccatt
    14581 gacgaccacg gccatctcct ttgataacac gtccacacat gctgtatggt cttcccaaca
    14641 acttggctgc gaaacgcaaa ttcccctgtt gcaacgcttc tctgattctc gtcgaactga
    14701 ttttttcatt ttctataaga aaatcagatt gtactgtcac ttcacaggaa taattagcac
    14761 ctagtgtttt gagtaaatta acatcacctt ctctgctttt gccaaaacga aaatcctggc
    14821 caaccagaag gtagcgtatt ttcaatgctt caaataaata aaactgagca aaatacaaag
    14881 cggaagtttg agccagcctt gcatcaaact tgatgcaata gatgtaatcc acctgacata
    14941 agtttaaggc atctattttt tctctcaagg tagaaagtct tgctggtgct ttctctctgt
    15001 gaaaatactc tttaggctga ggctcaaaca ataaaataac taaaggcaag ttcatttcat
    15061 cagcctttgc tcttagggcc ttaatcaaat gctgatggcc taaatgcacg ccatcaaaat
    15121 tgccaatagt ggcaactgca cccttatcaa atatagcaaa atgagtagaa ccgcgtaaca
    15181 atttcatgaa tcgttttaat cagaagaata aaaaacacat agtatactgg ttttctgaga
    15241 tattgagaaa tctataaagc attatccgta ctaccaagta gtacggaatt tcttgagtta
    15301 gaaggtgctc ttagtggtgt attttgggtc ttacaatcaa gacatcgcat tcagcaccgt
    15361 gcaagattgc attagcggtt gagcctaata ataaagaaag gccatgtttt ccatgacttc
    15421 ctgtcacaat taaatcaatt ttatgttctt ttgctaccct taggatttca tttttagttg
    15481 aaccatactc tataaaacga tgcttttcat ctacaccaag tttttctgcc aattcattca
    15541 gctctttttc agcttgctca cgaatagaca tttctacttc tgcaaatcca gcaaaacctg
    15601 ggtaagcata agctggaata ggctctacaa catgaactaa aattaaatca gcaccattct
    15661 cgtcagcaat ttttttagct ttctcagctg ctcgcacccc aacctcatca aagtccgtag
    15721 caaacaatac ccttttatac atctttttct ccttaaaatt ctgatagcta agttaagatt
    15781 agcagattta agcctatttt cccagaatca atcaacaact aacggctata tccaagctga
    15841 ttatcttagt ctaaccaatg tttgaaagtc cctccacaga tcgtatccat ggacttagtt
    15901 tggctaattc gcctggtagt ttttttatat ttagcagagc ttgattcctg ctctcatatt
    15961 caccgatagt taatatgtac caactacctt tcgcattggt aaaatgtctt atttttgcct
    16021 gagctaacca tttgttttgg ttcttaaacc tgttgatatc ctctacttta tgactggcag
    16081 ccaattgtat agtaaacagc ttactattaa ttttcttgat gcctggttgc tcactggcta
    16141 attttgtttg tgatgaatgg ggaataggag gtaattttga ttgactaact accgcttgcc
    16201 ctacctgttc tgaaatgttt ttagctttca gttttggaat gacaacgacc ttatctacaa
    16261 tagctactgt attaagggta tcctcttcat ccataaaatc atctaaaatt tgttttttgg
    16321 gtaacccata atgcactaac agcctcgttg aagaatcttc ccatgaagca atatagctta
    16381 cagagtcttg tttagggact gcttgttgtg tgggagtttt tataaggtca tttatgggtg
    16441 gaagtgttgc tacagaagct aaatctggca gtttgggagt attatcaggt gctttataga
    16501 cattatcaaa ataaaaatag gacaatcctg caacaatagc gaagctcgcg actaagctta
    16561 ttctttttaa aattgccttt ttattatttt gcttttcatt ggcgcacttg gataaaaata
    16621 attccaggtt tgaattgatt tttgataagt tccctttggt taagtgataa aactgtttaa
    16681 attgcgcgtc agtaagtggc ctgttgatta aatgagaagc cattgctctt tgtaatacat
    16741 aagttctggt ttcagtctcg cttaatgctc cgagttctat tgtgtgtatt aaattattaa
    16801 atggctcgac agcaagatta ttcaatgtgg caacgataga ataatcagaa attaaacaaa
    16861 gatggaaaaa tccaaaatct tcctgatttt ttataataat catcacttcc ttgataaaat
    16921 tttcaggcaa gtgatgtgca tcatcaataa tcagcaaaac atgcgttttt ctttcattaa
    16981 tttgtgctgc aagagaacca atattgttat ccttattatt aagatgcaac tgcctggcaa
    17041 actcattgat aatatcttga ctgtcaaatg gcggttctgc cttaaatgat actgatttaa
    17101 tttgttggtt caaatggctt ttcaataaag agttaaaaca agtttttccg ccctctttct
    17161 ctgacaaaat ggttattaga acattattaa agagaactaa atgattaatg aaatcaactt
    17221 tcgcaagcca ggctcctggt ttgaatagag ttctgggctg attggcaact ccctcatcaa
    17281 gaggctcctt tccatctatc
//
LOCUS       DAXLDT010000060        17251 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXLDT010000060 DAXLDT010000000
VERSION     DAXLDT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.060"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(242..1279)
                     /locus_tag="ACF2SP_001601"
     CDS             complement(242..1279)
                     /locus_tag="ACF2SP_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HFD2359390.1"
                     /translation="MVMRSFSFYYQHLENVSRSFSFCISQLTSPAKEWVALSYLLLRV
                     ADSIEDAKWQDLQTQSDSFAAFKSFLVQTPANDQFTKWLSSFPSQLPLGEKQLLCAVP
                     LLLEEKDNLPESIKQSVIKTILQVVDGMHYFLNHYHMEGKIVFPSLVTTNQYCFFVAG
                     LVGKLLSHIFTDLLSNFKWTATLLNQAFHFGFFLQKINLLKDKVDDETSGRYFISSEN
                     SLRESLVIHAHHSLAYVKSIPIIEGRPYRLFCAWSLFIGLASLKWLDKYGHRQKIKPR
                     ETYYLVNQINQLIDDNRALEKLFNSYLPGQYEEGSRYASESTKKIPTWFKKIYDGEMD
                     SLTLFDLDIEV"
     gene            complement(1279..3081)
                     /locus_tag="ACF2SP_001602"
     CDS             complement(1279..3081)
                     /locus_tag="ACF2SP_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFD2359391.1"
                     /translation="MIQLGDLLKPTWAAEQSLNNAWSVLNDEEKETIKSRMDKIFYNE
                     IPFQLEHDKLIYIHLFSLFAQLETIGLRGLIKSLEKLRGTDLYQQMRQQITDEIFHAT
                     VFAKVAFQLSAPYALPLGHQKSIKHFISSLEGEEDLATSITLVNLVGEGWVEELCIAM
                     KEKNIAVTIFETVLEDESRHMDEYDLYRQIGLPNKDYLRKKLAIFEDELINTVFAHEQ
                     YLTTLGILLGKEGALKLLNNINNKHHWMLKKIGLTPSAHWQLFMDTMPLLMKNLSHDF
                     EKDKAIEPTNIRKLLSAIWNDPELPTESAIFNINVTPVCFFEKKFKPETITCLMLQAL
                     SKACFDNPQTRNYIFNHKLYHSHNSYVALAVKIPGSDQLGAIEFKNCHEMTMTELAQH
                     IQHDMLIMKYCYEKTQSLQKEHPYLIEVVNRLLTPRHERVYRDFLFARPAISLSNIGH
                     WGYQAAVSPLFPNETFKITLTEIERKQVWNKTNNTFEVQDVLPVGMSVDHRVFDGNIP
                     FPRYMQEAFDQMFQDMEQSRIKPLSKPFSNLDSFIKYSNTLLENDLEFGFLYLFSLMH
                     VWKNYISYDELSKTVEENYERIKQALSKSEHQLG"
     gene            3688..3957
                     /locus_tag="ACF2SP_001603"
     CDS             3688..3957
                     /locus_tag="ACF2SP_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359392.1"
                     /translation="MNSKVFSQRFNRELATLGFPEDLTEKTKAVSKVFGVTRHLANAM
                     IFGHVLPDKEQLDKIAEILEVCPQWLSGATDRKKAYSGRETADSV"
     gene            4056..4862
                     /locus_tag="ACF2SP_001604"
     CDS             4056..4862
                     /locus_tag="ACF2SP_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HFD2359393.1"
                     /translation="MECLSFCVAKTIDLTRLDLHLKNVSKEFSAVKTRDVIRLNSHRN
                     KDHTLFIFKNGTVVSWGVKRYQIHEYLDIIKLLVDKPVALLVHDEFHYQIGDKTAIEP
                     HGFYDVDCLTIEEDSDELKLSLSYGFSQSVKLQYFETIIDALIEKYNPLIQALSHKGE
                     MPISRKQIQQVIGEILGAKSELNLISNFLYHPKYFWQHPTLEEHFSMLERYLHIQRRV
                     NAINHRLDTLNEIFDMFNGYLESRHGHHLEIIIIVLIAVEIIIAVMNFHF"
     gene            4880..6160
                     /locus_tag="ACF2SP_001605"
     CDS             4880..6160
                     /locus_tag="ACF2SP_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFD2359394.1"
                     /translation="MPTLKSYFLKDTSLRTEILAGATTFLTMVYIVFVNPKVLHDAGM
                     DQGAVFTATCLITAFACALTGFIANTPIGVAPSVALNIYFSYSVVQGMGVDWQHALAM
                     VFISGLLFLFLTLTKLRRLLIEAIPHNLQIAIVIGISLLIALIALENNQIIIGDQHNL
                     MRLGPIIRPETGLFFLGFILILTLDYHKIPGAIIIGILSISILSLMLGLTSWQGLIAM
                     PPSLEPTFLQLDFSGLSSIVALKTIFTFFLITVFDATGSLIGLLSPTIFKHEKNYYKR
                     LTRSLTADAAASSLAGLMGSASTSPYIESATGIEAGGRTGLTALVIATGFILMLFLFP
                     LAKMIPSFAVGPALLYVACCMIKHITNMKLQDISETAPCMVIIMMVPFTASIADGIGL
                     GIILYTLLKLFTRQKPNPLIIVLSLVFIVFFLIN"
     gene            6483..7079
                     /locus_tag="ACF2SP_001606"
     CDS             6483..7079
                     /locus_tag="ACF2SP_001606"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFD2359395.1"
                     /translation="MLEQSLRHYYQLVLVDPILPALRDRITPLSITWLSGIFGLLFIP
                     FVLLDKPYIAISFLLLSGYLDTLDGSLARFQNRASDFGSAMDIVMDRIVEFSAVLALY
                     LFDPQHRATLAILMLGSILLCITSFLVVGIFSNNTTQKSFYYSPGIMERAEAFLFFIF
                     MTLFSSCFNFLAALFCFLVCLTALIRLFEFKKSVQAPG"
     gene            7187..7762
                     /gene="msrA"
                     /locus_tag="ACF2SP_001607"
     CDS             7187..7762
                     /gene="msrA"
                     /locus_tag="ACF2SP_001607"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFD2359396.1"
                     /translation="MLRIFLYFLLLINTTFCLAKTEKAIFAGGCFWCMEADFDHLPGV
                     LSTTSGFDGDTLKNPTYEQVSAGNTHYAESVMVEFDPNKLSYKKLVEYFFMHIDPTVK
                     NAQFCDHGSQYRSAIFYLNDQQKKIAMEYKKELEQKFPTVYTEIVPSTSFYPAEEYHQ
                     NYYKKNPVRYKYYRYRCGRDARIQEVWGNKK"
     gene            7881..8741
                     /gene="vipF"
                     /locus_tag="ACF2SP_001608"
     CDS             7881..8741
                     /gene="vipF"
                     /locus_tag="ACF2SP_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector N-acetyltransferase VipF"
                     /protein_id="HFD2359397.1"
                     /translation="MITQVNQLDELQLKDLKALRAECKKNDGSIPNLYIHILKQHRSL
                     PTSFLYYHNGTLIGFLSVYFFYDDAVEVAVLVSPQYRRQGIAKQLIKEALPLIKSQNY
                     FNLIFSCPSRLNDNWLTQKGFTYLHSEYFMERDDLNPILDYIRPLSFRMATLEDIPIL
                     CGLDEVCFPDKNQDSVNRFQQILNEREYEIVIAMLNNHPIGKAHIRWQTKRATLSDIA
                     ILPKEQGKGFGSALIAHCINMILSEGKSRVDLDVETHNKKALNLYIQLGFHIQNACDY
                     WSINVNQLAK"
     gene            8866..10095
                     /locus_tag="ACF2SP_001609"
     CDS             8866..10095
                     /locus_tag="ACF2SP_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFD2359398.1"
                     /translation="MKKRVVITGMEITSSIGSGIDKFWLAASQGQCGIKRIQSYDPSP
                     YSTQIAGEITDLSLDHLPEFNKSKRYPRAAQYALFCAHHAIEKAGLEKAELNAAGTFI
                     GTSIGGQPELEAGYKLFFTDSWKKIPPLSVIRGMPNSIANHIAIAFGIGGPNSTISNA
                     CVSSGEAIGMAYQQIAHGNLTTALCGGTESLVWESVMAAWCKLRVMSTNNENPSQASR
                     PFDLNRDGMVMADGAGILILEELQQAKARGAQILGEIIGYGASCDAFHVTAPNSQGQA
                     RAIVAALNDAKLSINDVHYINAHGTGTQLNDSTETETIKMVFGKRAYDIPITAQKAMT
                     GHAIGAAGAMEIIATTLSLQKNTLLPTINLHTPDPACDLDYVPNAAREKTIDIALSNH
                     FAFGGANAALVLRKYIS"
     gene            complement(10092..10874)
                     /locus_tag="ACF2SP_001610"
     CDS             complement(10092..10874)
                     /locus_tag="ACF2SP_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFD2359399.1"
                     /translation="MLKKILLTGLCVLFSMNLSASEPIYDEEIPLLWSSIITVSGGPS
                     WATPGQNQYIYPMSPVYINNFNPNLPPNYYLYFTHDSPTTILANGEIFFGLQRAINPQ
                     MIGQLGLGVAGVSDAKVTGDVNLNGLLNVYTYDYKVNHARVELKGKLIGSAFTPVQPY
                     LSGSFGIAFNNAHDYRTLSINQFLFPAPWFASNTTYAFAYSVGAGVQKMLNRNWQVGV
                     GYEFADLGKSYLGGDGVNITKGPRLTHLYNNQLLFSVSYLYS"
     gene            11138..12193
                     /gene="lpxD"
                     /locus_tag="ACF2SP_001611"
     CDS             11138..12193
                     /gene="lpxD"
                     /locus_tag="ACF2SP_001611"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFD2359400.1"
                     /translation="MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGE
                     DNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKAFIALLNHFYPPRRISPGVHPTA
                     VIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
                     NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEIGANTAIDKATL
                     GATVIGEGTKIDNLVQIAHSVKLGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVH
                     IEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTE
                     QVAVINKKLDIKAKEVE"
     gene            12196..14886
                     /gene="polA"
                     /locus_tag="ACF2SP_001612"
     CDS             12196..14886
                     /gene="polA"
                     /locus_tag="ACF2SP_001612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFD2359401.1"
                     /translation="MKPPLILIDGSSYFFRAFHALPPLTNSKGQPTGAIYGVANMVKK
                     IIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPL
                     LIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG
                     VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLAHANEIG
                     GKIGEYLRASIPHLPLSKKLVTIKTDLELPLNLNQLMPKPADNEQLIKLTRELEFKSW
                     LKELLQEEENQKSPKEMDASNNKSYEIITTHQQLNHWLNKLEQSQQFCIDTETTNLDV
                     MQAELVGISLAVEEDNASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKY
                     DYSVLKNYGITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGA
                     KQLRFDQIPVEKAGIYAAEDADITLRLHHKLYPMLDKPLRNVLHDIEMPLLTVLADME
                     MHGVLIDPVILEKHGSRLKEQMKSLEQEAVQLAGKAFNLNSPKQLQEILFDEQKLPVV
                     AKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHT
                     SYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMA
                     HLSQDDNLLRAFANGWDIHAATASEIFQTNLEAVSKEQRRRAKAVNFGLIYGMSAFGL
                     AKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSR
                     NLMRQKAAERTAINGPMQGTAADIIKKTMLAISSWEHSQKSPSAKMIMQVHDELVFEV
                     RKEAVEECHAIIHNLMEQTVKLSVPLVVSIGTGSNWDDAH"
     gene            complement(14916..15368)
                     /gene="lerC"
                     /locus_tag="ACF2SP_001613"
     CDS             complement(14916..15368)
                     /gene="lerC"
                     /locus_tag="ACF2SP_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator LerC"
                     /protein_id="HFD2359402.1"
                     /translation="MRVLIVEDSAFNAFCLRRLLESVVASISVTIVNNSQAALSFVNN
                     YCPDIVVVDGDLGASPDEMNCNGPELAGILLQKYPQLPIVAWSDSDAMREDFIKVFKS
                     HGLFLNEYNTWPKVIGLERIHKTWSYYFGEFIGGQNPSFASQTSINYY"
     gene            complement(15483..15806)
                     /locus_tag="ACF2SP_001614"
                     /pseudo
     CDS             complement(15483..15806)
                     /locus_tag="ACF2SP_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
     gene            complement(15978..17171)
                     /gene="ceg4"
                     /locus_tag="ACF2SP_001615"
     CDS             complement(15978..17171)
                     /gene="ceg4"
                     /locus_tag="ACF2SP_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector phosphotyrosine
                     phosphatase Ceg4"
                     /protein_id="HFD2359403.1"
                     /translation="MPKVIIFTDFDGTVTGKSGNETVFTEFYQSLLQGYKKDVEQDYK
                     NTPMKDPIEAQALFEAKYGKYNENFDHDQQDVDFLMSPDAVALFHEVLKNDDVTVNIV
                     TKNRAEYIKAVFKYQGFSDEEIEKLTILESGYKFNDVNSRLNHPTERANRVYILDDSP
                     TDYAEMLRAVKGRGYNEEEIRGYCKNPGEFEWSQYLEDVREMFPPKENSPEEVRLPYE
                     QGDEVSIGEETPALLQEDNLSQREETATVLSNQPVPSSDFRTLKIMGAFAGIGFGFGF
                     ALGVTLVATGVFAPSGVGLLGALTLGAISGTGAAALSSAIGFAVAKGTDITPEPVSEE
                     PVPAVKQSSHEAMRGLGGSQLLSTNNIHPVGHFPSVLSRRTPTKGKEEQCQEEYVLTN
                     PLNNS"
BASE COUNT         5444 a         3447 c         3171 g         5189 t
ORIGIN      
        1 actcaaataa ataggcttat aaacagaaaa atttgcatac cccgtgtacg gttacaccat
       61 aagagcaggg agtcactaag ccgtgtgcac tgccgttaaa tttgtctgac tcaataactg
      121 gttataaaga gaaaatcgcg tgatggggaa accagatcag gcaattgctc agccaattgc
      181 aggtataatt ttgtttaaaa ccgtccataa atttgtggca gtttgcacca aatctccctc
      241 atcatacttc gatatccaga tcaaacaatg tcagagaatc catctcacca tcgtaaattt
      301 tcttaaacca agttggtatt tttttcgttg attcagaagc ataacgtgaa ccttcttcat
      361 actgtcccgg aagatagctg ttaaataatt tttctaaagc gcggttatca tcaattaact
      421 gatttatttg gttaaccaga taatacgttt ctcggggttt aattttttgt cggtgaccat
      481 atttatcaag ccatttcaat gaagccaagc caataaataa agaccaggca caaaataagc
      541 ggtacgggcg tccttcaata attgggatgc tctttacata agccagtgaa tggtgtgcat
      601 gaataaccaa actctcacgc aagctattct ctgaagaaat aaaataacgc ccacttgttt
      661 catcatccac tttatctttc aatagattga ttttttgtaa aaagaaacca aagtgaaagg
      721 cttgatttaa cagagtggct gtccacttaa aattagatag taaatcagta aatatgtgcg
      781 acaataattt gccaaccaat cctgcaacaa aaaaacagta ttgattagtg gtaactaatg
      841 aaggaaatac aattttgcct tccatatgat aatgattaag gaagtaatgc attccatcca
      901 ctacttgcaa aattgttttt atcacagatt gcttgatgga ttcaggtaaa ttatcttttt
      961 cttccaacaa aagagggacg gcacatagaa gttgtttctc ccctaatgga agctgcgaag
     1021 gaaagctgga aagccatttt gtgaattgat cgtttgccgg tgtttgaact aaaaatgatt
     1081 taaaagcagc gaatgaatca ctttgtgttt gaagatcttg ccatttggca tcttcaatac
     1141 tgtctgcaac acgcaaaagt aaataactca acgcaaccca ttcttttgct ggagaagtta
     1201 actggcttat acaaaaacta aaacttctgc ttacattttc tagatgttgg taataaaaag
     1261 aaaacgatcg cataaccatc agccaagttg atgttcgctt ttcgataatg cctgttttat
     1321 tctctcgtaa ttttcttcca ctgtttttga caactcatca tatgaaatat aatttttcca
     1381 tacatgcatt aaagaaaata aatacagaaa cccaaactcc aggtcgtttt ccaataatgt
     1441 attactgtac ttgataaagc tatctaaatt tgaaaagggt tttgacagtg gctttatcct
     1501 ggattgctcc atgtcttgaa acatttgatc aaaagcctcc tgcatataac gtgggaacgg
     1561 gatattgcca tcaaaaactc ggtgatcaac actcatgcct acaggcaaaa catcttgcac
     1621 ctcaaatgtg ttatttgttt tattccagac ttgttttctt tcgatttctg tcagagttat
     1681 tttaaacgtt tcatttggaa ataagggtga aaccgctgct tgatatcccc aatgaccaat
     1741 attgcttaaa gaaatagcag gtctggcaaa taagaaatca cggtacactc gctcatgccg
     1801 tggagttagc aagcgattga ctacctcgat taaataggga tgctcttttt gtaatgattg
     1861 tgttttttca taacagtact tcatgatgag catgtcatgc tgaatgtgtt gcgctaattc
     1921 agtcatagtc atctcatggc agtttttaaa ctcaatcgca cctaattgat cactacctgg
     1981 aatctttacc gcaagcgcta cgtaactatt gtgcgagtgg tacagtttat gattgaaaat
     2041 ataatttctc gtctgcggat tatcaaaaca agctttactc aatgcttgta acatgaggca
     2101 agttattgtt tccggtttaa atttcttttc aaaaaaacaa acaggagtaa catttatatt
     2161 aaaaatagca gactcagttg gtaattcggg gtcattccaa atcgctgaaa gtaatttacg
     2221 aatattggtc ggttcaatgg ctttgtcttt ttcaaaatca tgcgataaat tcttcatcaa
     2281 taaaggcatg gtatccataa acaattgcca atgggctgaa ggcgttaagc caattttttt
     2341 aagcatccaa tggtgtttat tattgatatt attgagcagt ttcagtgccc cctctttgcc
     2401 taatagaata ccaagggtag tcaaatattg ctcatgcgca aatacggtat ttatcagctc
     2461 atcttcaaaa attgctaatt ttttccttaa atagtcttta tttggcaacc caatctgacg
     2521 atataaatcg tactcgtcca tatgccggct ttcatcttca aggactgttt caaaaatagt
     2581 tactgcaatg tttttctctt tcattgcaat gcataattct tctacccatc cttctccaac
     2641 caaattgacc aaagtgatag aagtggccag atcctcttca ccttcaagag aagaaataaa
     2701 atgtttgata ctcttttgat ggccaagtgg taatgcgtaa ggagcggaca actgaaacgc
     2761 aacttttgca aaaacagtgg catggaaaat ctcgtcggta atttgttgac gcatttgttg
     2821 atacaaatct gttccacgga gtttctctag agattttata agccctctta ggccaattgt
     2881 ttctaattga gcaaacaagg aaaataaatg aatatagata agcttgtcat gctcaagttg
     2941 aaaaggaatt tcgttgtaaa atatcttgtc cattcggctt ttgatggttt ccttttcctc
     3001 gtcattcaat acagaccaag cattatttaa agactgctcc gcagcccaag ttggttttaa
     3061 caaatcgcct aactgaatca taaattaacc ccaatccatt tgttatattg caaacgccta
     3121 cactcttccg gagcacatcc taatatttaa ggggaagtgt actattgaaa gcgttttttg
     3181 tggatattgt catatactga ttgtaattgc tactggattc cagcctgaaa ataaccatag
     3241 ataaaaaaag acttcttaaa attatcttat cgatgagcga taaaacccct ttgatcaatg
     3301 agttattcgc aaatctcgag agtctgagat aactggattt cggcccactc cgacttctct
     3361 acagggtaga caaagtacga cctgatatct catgcaaacc aaagtacgtt agctgattga
     3421 cgagtcattt aaaatcaaat ctttataata aaataagcat cccagaactg tttgcaatgc
     3481 agcaatataa caaaagggac agattagaga gaggagctat tatctcaatg gaaaataatt
     3541 gattttaata gcaaaaatat ctttgctatt aaaaaaaaat aagttataac actcttaact
     3601 acatcaagga ttgattagtg caaatgttgg gtcaacacct caataaattc atgttgagtt
     3661 ggctgtataa ttttcatgga gtgaaaaatg aatagtaaag tattttcgca acgttttaat
     3721 cgtgaattgg caactctggg attccccgag gatttaacag aaaaaacgaa agccgtatca
     3781 aaagtatttg gtgtcacacg ccatttagct aatgcaatga tatttggcca cgttcttcct
     3841 gataaagaac aactggataa aatcgcagaa attctggaag tttgtcctca atggctaagt
     3901 ggggcaacgg ataggaaaaa agcctactca ggccgtgaga cggctgatag tgtgtaataa
     3961 acctggaatt tcatgtatac tgtaagctaa tagacagcgt ttgctaaaat tatttacttt
     4021 gcaaacgctt ttttcattgt tgggttatta aaatcatgga atgcttaagt ttctgtgtgg
     4081 ccaaaaccat agacttgact cgtcttgatc ttcatctaaa aaacgtctct aaggaattta
     4141 gtgctgtaaa aacccgagat gtaataagat taaattctca tagaaacaaa gatcacaccc
     4201 tgttcatttt taaaaatgga actgtcgtat cctggggtgt aaaaagatac caaatccacg
     4261 aatatctgga tattattaaa ctgcttgtag acaaacctgt agcacttttg gtgcatgatg
     4321 aatttcatta tcaaattggt gataaaacag ccatagaacc gcatggtttt tatgatgtgg
     4381 attgcctgac cattgaagaa gacagtgatg aactaaagct cagtttgtct tacggctttt
     4441 ctcaatctgt aaaattacag tatttcgaaa ccattattga cgcattgatt gaaaaatata
     4501 atccgctgat tcaggcctta tctcataaag gagaaatgcc tataagcaga aaacaaattc
     4561 aacaagttat tggtgaaatt ttaggtgcca aaagcgagtt gaatttgatc agtaatttct
     4621 tataccaccc caaatatttt tggcaacacc ccactctaga agagcatttt tccatgcttg
     4681 agcgctattt acatatacaa agacgcgtca atgccattaa ccatcgtctt gatacgctta
     4741 atgaaatttt cgacatgttc aatggttatt tggaaagtcg ccatggccat cacttggaaa
     4801 taattattat tgtattaatc gcagtagaaa ttataattgc agttatgaat ttccattttt
     4861 agggtttttc ccttaattta tgcccactct aaaatcctat tttctcaaag acacctcttt
     4921 gcgcacagaa atacttgccg gagcaacgac cttccttacc atggtttata tcgtttttgt
     4981 caatccaaaa gtcctgcacg atgcaggaat ggatcaaggc gcggtattta ccgccacttg
     5041 tttgataacg gctttcgcct gtgcattaac aggcttcata gccaacacac cgataggtgt
     5101 agcgccaagt gtggctttaa atatttattt ctcttacagc gtagtacaag ggatgggagt
     5161 tgactggcaa cacgccttgg ccatggtatt catttccggc ctgcttttcc tgtttcttac
     5221 cctcaccaaa ttaagacgcc tcctgattga agcaatccct cataatttac aaatagccat
     5281 tgttataggt atcagtttgc taatcgcctt aattgcttta gaaaataatc aaatcattat
     5341 tggtgaccag cacaatttaa tgcggttagg gcctatcatc agaccagaaa caggactctt
     5401 tttcctgggg tttattctta ttcttactct ggattatcat aagataccag gtgccatcat
     5461 cattgggata ctgagcatca gtatcttaag cttgatgttg ggcttgacca gttggcaagg
     5521 tttgattgct atgccaccgt cgctcgaacc tacgtttttg cagcttgatt tttctggtct
     5581 ttccagtatc gtggctttaa aaaccatttt tacctttttt ctgatcaccg tgtttgatgc
     5641 gacaggatca ttaattggct tactaagtcc aaccattttt aaacatgaaa aaaattatta
     5701 taaacgtcta actcgcagtt taactgccga tgcagccgca tcttccctcg caggcctcat
     5761 gggctctgcc agtacctcgc cttatattga gtcagcaacc ggaattgaag ccggtgggag
     5821 aaccgggcta actgcgttag tcattgcaac tggatttatt ctgatgctgt ttctttttcc
     5881 acttgccaaa atgattccaa gctttgcggt tggaccggct ttactctacg tagcgtgttg
     5941 catgataaaa catattacta atatgaaatt acaggatatc tccgaaacag caccctgcat
     6001 ggtcatcatc atgatggtgc cattcaccgc ttcgattgcc gatggaatag gtttgggaat
     6061 tatactttat acccttctta aattatttac tcgtcaaaaa cccaatcctt tgattattgt
     6121 tttaagcctt gtttttatcg ttttcttttt gattaactaa gtgaatgtga tctttaaatc
     6181 ctggataaac cgaaaagctt ccataaaaaa cttgtctact ctcaaaaact gcaaacaaaa
     6241 gtttctttaa tttattgaat ttagtagtat ttaaataagt gaattatgca caagatgggt
     6301 tgttatctta aatcgttcgt ttaatatttc gtgccaaagc ttcgagacga tgcttagcca
     6361 tctcctcaac ccaaatgggc atcggatatc gaatgcttct tatataatac ttgtccataa
     6421 ctcacgttag atagcaaaca actgccataa gctcatagtt ggcctaatcg cgatgaatta
     6481 ttatgcttga acaatcatta cgacattatt atcagcttgt cttggtagat cccatactac
     6541 ctgctttacg tgacaggata acgccattgt ccattacctg gttgtcggga atatttggct
     6601 tgttgtttat tccttttgtc ttattagaca aaccgtatat tgcaattagt tttctcttgc
     6661 tttctggata cctggatact cttgatggtt cgctggcccg ctttcaaaac agagcctctg
     6721 attttggctc agcaatggat atcgtcatgg atagaattgt agaattttct gctgttcttg
     6781 ctttgtatct ttttgatcca caacaccgtg ccactttagc cattttaatg ttaggcagta
     6841 ttttactctg tattacaagc tttctggttg tgggaatttt ttccaacaat actactcaaa
     6901 aaagttttta ttacagcccg ggaataatgg agcgtgccga agcatttcta ttctttattt
     6961 ttatgacatt attttccagc tgctttaatt ttcttgccgc tctcttttgt tttttagtct
     7021 gtttaactgc gttgataagg ctttttgaat ttaaaaaaag cgtacaggct ccaggttagc
     7081 gaaacaataa gtttcccgaa gcctgattta aaactttctc tgattttatg ctgactccaa
     7141 tacaaaatct tgtataattt acagcaattc ataaaagagg cttacaatgt tgcgtatctt
     7201 tttgtatttt ctcttactca ttaacacaac attttgtctg gcaaagactg aaaaagccat
     7261 ttttgcaggt ggatgttttt ggtgcatgga agcagacttt gaccatcttc ctggagtcct
     7321 gtcaaccaca tcaggatttg atggagacac tcttaaaaat ccaacttatg aacaagtttc
     7381 tgccggtaat acccattacg ccgaatcggt aatggtagag tttgatccca ataaattaag
     7441 ttacaaaaaa ctggttgagt atttctttat gcatattgat cctaccgtca aaaatgcgca
     7501 attttgtgat catggcagtc aatatcgcag tgcaattttt tatcttaacg atcaacaaaa
     7561 gaaaattgcc atggaataca aaaaagagtt agagcaaaaa ttccccacag tttatacaga
     7621 aattgtccca tcaacttctt tttatccggc agaagaatat catcaaaatt attacaaaaa
     7681 aaatcctgta cgttataagt attatcgata tcgttgtggc cgagatgccc gaattcaaga
     7741 ggtttggggt aataaaaaat aaaagaatag ctcttaatca tgatccatag ttttatcgct
     7801 tatcaaaaaa gaataagcaa ttcatgagaa aacacgctaa tctaataaat aataattttt
     7861 aattttttac taatacaatt atgattactc aagttaacca attagatgaa cttcaattaa
     7921 aagatttaaa agcattaaga gccgagtgta aaaaaaacga tggtagcatt cccaatctat
     7981 atatccatat attgaaacaa caccgctctc ttcctacaag cttcctttat tatcataacg
     8041 ggaccttgat tgggttcttg agtgtttatt tcttttatga tgatgccgtt gaagttgctg
     8101 ttctggttag tccccaatat cgcagacaag ggattgccaa acaattaata aaggaagcat
     8161 tacccttaat taagtctcaa aactacttta acttgatttt ttcatgcccc tcacgcctca
     8221 atgataactg gttaacccaa aaaggattta cctacctgca tagcgaatat ttcatggaac
     8281 gtgatgattt aaatcctata ctcgattaca tacgtccatt gtcctttcgc atggcgactt
     8341 tagaggatat tcccatatta tgtggcctcg atgaagtatg ttttcctgat aaaaaccaag
     8401 actcggttaa ccgattccaa caaattctta acgaaagaga atatgaaatt gtaatagcca
     8461 tgctcaataa tcatcccata ggaaaagccc atattcgctg gcaaaccaaa agggctacct
     8521 tgtctgatat tgccatattg cctaaagaac aaggaaaagg atttggttct gccttgattg
     8581 ctcattgcat caatatgatt ttgagcgaag gcaaatcccg ggtggatctt gacgttgaaa
     8641 cccataataa aaaggcatta aatctgtata ttcagcttgg atttcatatt caaaatgctt
     8701 gtgattactg gtcaattaat gtgaatcaac ttgcaaaata aaactgtata ttctttcgtt
     8761 acaaatcaca tattataaat atggcttgac ataattaaaa tctcaccatt agtattgcga
     8821 atggcgatag aggaaggctt gttttatcaa aaataaggat aattaatgaa aaaaagggtt
     8881 gttattactg gcatggaaat aacgtcatcc ataggatcgg gaattgataa attttggctt
     8941 gctgcatctc aaggtcaatg tggaattaaa cgcatccaat cttatgatcc atccccctat
     9001 tcaacccaaa tagcaggtga gattacagac ttatctcttg accatctccc cgaattcaac
     9061 aaaagcaaac gttatcctcg tgctgctcag tacgcattat tttgcgcaca tcatgccatt
     9121 gagaaagcag ggcttgagaa agctgagctc aatgcagcag gtacctttat aggcacaagc
     9181 attggcggcc aacctgaact ggaagcaggt tacaagttat tctttacaga cagctggaag
     9241 aaaatccccc ctcttagcgt gattagggga atgcccaatt caatagccaa tcatatcgca
     9301 attgcttttg gcataggtgg accaaactca accatttcca atgcctgtgt ttcatccggg
     9361 gaagcgatag gcatggctta ccaacaaatt gcccatggca atttgaccac agctctttgc
     9421 ggaggaacag aatctttagt ctgggaatcg gttatggctg cctggtgtaa gcttagagta
     9481 atgtcaacta acaatgaaaa tccctcacaa gccagcagac cgtttgatct caatcgggat
     9541 ggaatggtta tggcggatgg ggctgggata ttaattcttg aagagctaca acaagcaaaa
     9601 gcaaggggag cacaaattct cggagagatc attggctatg gcgcaagttg tgatgcattc
     9661 catgtcacag caccaaactc acaaggacaa gcaagagcta ttgttgctgc tttaaatgat
     9721 gccaagttat caattaacga tgtccattac atcaacgcac atgggacagg aacccagctc
     9781 aatgacagca cagaaacaga aacgattaaa atggttttcg gtaaaagagc ctatgacatc
     9841 ccgattactg ctcaaaaagc catgacagga catgcgattg gggcagcagg ggcaatggaa
     9901 atcattgcaa caacactaag cttgcaaaaa aatacgctgt taccaacgat taacctgcat
     9961 actccagacc cagcctgtga tctggattat gtgcctaatg ctgcgcgaga aaaaaccatt
    10021 gatattgctt tatccaacca tttcgctttc ggtggagcaa atgccgcttt agttctgcgc
    10081 aaatacatta gttaagaata tagatagctt acgctaaaca gcaattgatt attatataaa
    10141 tgggtcaatc gtggcccttt ggtaatatta acgccatcac cacccaaata acttttccct
    10201 aagtctgcaa actcataccc tacgccaacc tgccagttac gatttaacat tttttggaca
    10261 ccggccccca cactgtaagc aaaagcataa gtggtgtttg acgcaaacca tggtgcagga
    10321 aacaggaatt gattgataga aagagtcctg taatcatggg cattattaaa tgctattcca
    10381 aaactaccgc tcagataagg ttgcacgggt gtaaatgcac ttcctatcag tttgcctttt
    10441 agctctactc gtgcgtgatt cactttatag tcatacgtat agacattaag gaggccattc
    10501 aggttgacat caccagtaac cttggcatcg cttactcccg ctactcctaa acccaattga
    10561 ccaatcattt ggggattgat cgcacgctgc agcccaaaga atatctcgcc attggctaaa
    10621 atagtcgtcg gagaatcatg ggtaaaataa agataataat ttggcggtaa attgggattg
    10681 aaattattaa tatagactgg agacattgga taaatatact gattttgtcc aggtgttgcc
    10741 cacgaaggtc ctccactcac tgtaataata gaggaccaaa gtaagggaat ttcttcgtca
    10801 taaatcggtt ctgaagcgga aagattcata ctaaacaaaa cacataatcc ggtgagcaat
    10861 atttttttca gcatatccca tccttttgat tacaaatcag ccctcttgat tcaggctctc
    10921 taatgaactg attttaatag aagttatttg attttatacc ctaattaatc tcatttttct
    10981 agggtgcgtt gacaattcat ctggatcatc agaatagcag gatcatgata agaaaaatta
    11041 ttaatctgaa agccatctga atcgaggata attcactcga aaatggacaa taagaggtat
    11101 ataatcgctc tattttgcgg aattgaaagg taaaccaatg aattcttctc ttcgtgatat
    11161 agcaaacctt gttcaaggta tggttattgg caatgatgcc atcactgttt ccacactatc
    11221 acccatagat aatatacttc ccggatcttt agttttcgcc gaaggtgaag acaatatcaa
    11281 acttgctgaa aactctgaag cagcagctat tcttattggt caaggtacta tcgattcccc
    11341 aaagccatta attcaggtaa aaaacccttt taaagcattt attgccttac tcaaccattt
    11401 ttatccccca aggagaatat ctccgggggt acaccccact gctgtaatag gtgccgaagt
    11461 acaattaggc gatgaagttt atgttgggcc attcgtagtc attgaatcag gaagtattat
    11521 aggcaaccac tcagtgctca aaagtcatat tcatatcggc cataatgtgg tcattggaga
    11581 tcatacaacc attcaccccc aagtcactat ttatgataat tgccgtatcg gttcgaatgt
    11641 cactatccat gcctctacag taataggttc tgatggcttt gggtatactt ttgttgatgg
    11701 ccaacatctc aaagtacctc attcgggata tgtggtaatt gaaaataatg tcgaaattgg
    11761 agccaacaca gcgatagaca aggccacatt aggtgctaca gtgataggtg aaggtacaaa
    11821 aattgataat ttggtacaaa tcgctcactc agtcaaatta ggtaaacata atattatttg
    11881 tgcttttact ggcattgccg gcagtaccac taccggaaac aatgtcatct ttgccgccaa
    11941 cgtgggagtg agtgaccatg ttcacatcga ggatgaggtc atattaggcg ctcgtacagg
    12001 agttccccca cacaaacact taaaaaaagg aacagtatat cttggtaatc cggcaaaacc
    12061 taaagatgtt gcgattaagc acgaactgtc agtgaaccgt atcccattaa tacgaaaaaa
    12121 cattaaatca cttaccgagc aagtggctgt aattaacaaa aaactcgata ttaaagcaaa
    12181 agaggtagag taactatgaa accgcctctg attctaatag acggatcttc ctatttcttt
    12241 agagcgtttc atgcccttcc accattgaca aactccaaag gccagcctac aggcgcaatt
    12301 tatggagttg ccaatatggt taaaaaaata atcaaagatt atcagccaga agaaatagcc
    12361 gttgtctttg atgccaaagg gaaaacattc cgagatgaat ggtatccaga atacaaggcg
    12421 cacagaccac caatgcctca agaattgagc tctcagtttc aaccgctaat ccagttactg
    12481 caagcaatgg gattgcctct gcttatcatc gatggcgtcg aagcagatga tgtgattggc
    12541 actttggcaa aacaagccac agagcaaggc ataccagttg ttatatcaac cggtgataaa
    12601 gacatggcac aattggtcaa tgaacatgtc tccctcatca ataccatgag taattacagt
    12661 atggatattg atggtgtcaa agcaaaattt ggtgtaacac ctgaacaaat cattgattac
    12721 ctcactctca ttggagacag tgtagataat attccaggag tagagaaatg cggccccaaa
    12781 acagctgtaa aatggctgac agagtatcaa acgcttgata atttgcttgc acacgccaac
    12841 gaaattggcg gcaaaattgg tgaatatttg cgtgccagca tccctcattt gccactctca
    12901 aaaaaattgg tcactatcaa aactgatttg gaattgccct taaatttaaa ccaattgatg
    12961 cccaaaccgg ctgataacga gcaattaatt aaattaactc gtgaattaga atttaagtca
    13021 tggcttaaag agttactaca ggaagaagag aatcaaaaat cacccaaaga aatggatgcc
    13081 agcaataata aatcctatga aataataacg actcatcaac agttaaatca ttggcttaat
    13141 aagctagagc aaagtcaaca attttgcatc gacacggaaa caaccaacct tgatgtcatg
    13201 caagctgaat tggtaggcat ctctttagca gttgaggaag acaatgcatc ctatattccc
    13261 cttgctcata ctgatggcag tacacagtta gtaaaagaag aagttttaac tgctttaaag
    13321 cctattttgg aaaatccagc tataggaaaa atagggcaaa acattaaata tgattacagt
    13381 gtccttaaaa attatggtat taccttaaaa ggcattaggt acgacaccat gctggaatcc
    13441 tatgtgctca atagtggtgc aggcagacat gatatggatt ctttggcttt aaaatacctg
    13501 ggttataaaa caataagtta tgaggatgtt gcgggaaagg gagcaaaaca actccgtttt
    13561 gaccaaattc cagtagagaa agccgggatc tatgcagccg aagacgctga cattactttg
    13621 cgattacatc ataagctgta ccccatgctg gataagccat tgcgtaacgt gttgcatgat
    13681 atcgaaatgc ctttactcac tgttctcgca gacatggaaa tgcacggcgt tctcatcgat
    13741 ccagtcattc tggaaaagca cggaagtcgt ttgaaagaac aaatgaaatc tctggaacag
    13801 gaagcagttc aactcgctgg aaaagcattc aatctcaatt ccccaaaaca attacaagaa
    13861 atactatttg atgaacaaaa actgcctgtt gtagcgaaaa cccccacggg acagccttcg
    13921 actgcagaat ctgttttaca ggaattggct tttgattatc gccttccggc tgtaattctg
    13981 gaataccgaa gcttatcaaa attggtcagt acctatattg atgccttacc caaaagaatc
    14041 aaccccaaaa cacatagagt gcacacatcc tataatcaag cggtagcagc aacaggtcga
    14101 ttatcatcca gtgatcctaa cttacaaaat atccctatac ggagtgaaga aggaaggttg
    14161 attcgaactg cctttatagc accagaagga agtcttatca tggcggctga ttactcgcaa
    14221 atcgagttaa ggataatggc gcatttatct caagatgata atcttttacg agcttttgct
    14281 aatggttggg acatccatgc tgcaacagcg agtgaaattt ttcaaacgaa tttggaagct
    14341 gtgtccaaag aacaacgaag gcgtgccaag gcagtgaact ttggtttgat atatggtatg
    14401 tctgcatttg gactggcaaa acaaattggt gtggagcgtc aggatgctca acattatatt
    14461 gatacttatt tccgccgtta tcctaaggta cttgaataca tggagcgtac tcgcaaacaa
    14521 gcgcatcaac ttggttatgt tgaaacctta tttggccgtc gcctctactt acctgaaatt
    14581 aattcacgca acctaatgag acaaaaagca gctgagcgca ctgccatcaa cggaccaatg
    14641 caaggaactg cagctgatat tataaaaaaa accatgctgg ctatttccag ttgggagcat
    14701 tcgcaaaaga gcccctctgc aaagatgatc atgcaagtac atgatgaatt ggtttttgaa
    14761 gtccgaaagg aagcggttga agaatgtcac gccattatcc acaatctcat ggaacaaact
    14821 gtaaaattat ctgtacctct tgttgtgtct attggcacag gttctaactg ggatgatgcg
    14881 cattgaagcg cataaaaatg tttaatttat tggatttaat aataattaat agaagtttgg
    14941 ctcgcaaagg aaggattttg accacctata aactcaccaa aataataaga ccacgtttta
    15001 tgaatacgct ccaaaccaat gactttgggc caggtattat actcattcaa aaacaggcca
    15061 tgactcttaa acactttaat aaagtcttct cgcatagcat cggaatcaga ccaggctaca
    15121 ataggaagtt gcgggtattt ttgcaataaa attcctgcaa gctcgggacc attgcagttc
    15181 atttcatcag ggctggcacc cagatcacca tcaacaacga cgatatcggg gcaatagtta
    15241 ttgacaaagg ataatgcggc ttgactatta ttgacaatag tcactgaaat tgatgcaaca
    15301 accgactcca acaatcggcg caaacaaaag gcattaaaag cactgtcctc aacaattaat
    15361 acacgcatca ctgctccctc ttaactacga aagaagtata atgtgcgaac cttaagaaaa
    15421 tcttaagaaa tagaattaat ccaattaaaa attacaaatt gaaaaatcaa cgaagttccc
    15481 tgttaaattt tttttacaaa ttgtgatttt aatttcatgg cgcctatccc gtcgatcctg
    15541 cagtcaatgt catgatcgcc gtcaaccaga cggatatttt ttactttagt tcctaccttt
    15601 ataacttgag aagagccctt tatttttaaa tccttgatga ccgttatcgt atctccattt
    15661 tgcaacagat ttccattggc atctttgaca atttttgcag actcttcgtc attgttacta
    15721 ctgttttttg gccattcatg cgtacactca gggcagatta atacatcacc gtcctcataa
    15781 cagtattcag aaagacattt agggcaattg gttcattttg ttgaaaaacc ctgaattaaa
    15841 ttgattacaa actattttac tctaatagaa agcattttaa aaatttttga ctaaattgac
    15901 ctgtaattga acggccctgg aatgaccgga taaataatga tttttttaat cattatttat
    15961 ccaacgaaat agaaatgcta tgaattattg agtggattag tcaatacgta ttcttcttgg
    16021 cattgctctt ctttaccttt cgtgggagtt cttcttgata gaacgcttgg gaaatgacca
    16081 accggatgaa tattgtttgt gctcagtaat tgagaaccac ccaaaccccg catagcctca
    16141 tggctgcttt gcttaacagc aggaacaggc tcttcgctta cgggctctgg ggtaatgtct
    16201 gttcctttag caactgcaaa tccaatagca cttgatagtg cagcagcgcc agtaccactt
    16261 atagcaccta aagtcaaagc tcctaaaaga ccaacacctg aaggagcaaa aactccggta
    16321 gcaaccaatg taacacctaa agcaaagccg aaaccaaagc caatgcctgc aaaagcaccc
    16381 ataatcttta gggtacgaaa atcagaagac ggaaccggtt gattagataa gacagtcgcc
    16441 gtttcttcac gctgggataa attatcctcc tgaagtaaag caggggtctc ttcaccgata
    16501 gaaacctcat ccccttgctc atatggtaat cgaacctctt caggagagtt ctctttaggc
    16561 gggaacattt ccctaacatc ttctaaatat tgggaccatt caaactcccc aggattttta
    16621 caatacccac gaatttcttc ctcattgtat cctctacctt ttactgcacg cagcatctca
    16681 gcatagtctg tcgggctatc atcaaggata taaacccggt tggccctttc tgttgggtgg
    16741 ttaagccggg aattgacatc attaaattta taccccgact ccagaatggt taatttctcg
    16801 atttcttcat cagaaaatcc ttgatatttg aaaactgctt taatgtattc agctcggttt
    16861 ttggtgacaa tattaacggt cacatcatca ttttttagta cttcatggaa aagcgccact
    16921 gcatcaggac tcatcaggaa atcaacatcc tgctggtcat gatcaaaatt ttcgttgtat
    16981 tttccatatt ttgcttcgaa taaagcctga gcttcaattg gatctttcat tggagtgttt
    17041 ttgtaatctt gctctacatc ctttttatag ccttgtaaca gtgattgata aaactcggta
    17101 aatacagttt cattaccgct ttttccggtt acagtaccat caaaatcggt aaaaataata
    17161 actttaggca ttgtatttcc ttactatttc tcttagtttt aaatgactcg atattgtaca
    17221 aggcttaaat taagagaata ttaagtacag a
//
LOCUS       DAXLDT010000061        17170 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXLDT010000061 DAXLDT010000000
VERSION     DAXLDT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17170
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.061"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            198..512
                     /locus_tag="ACF2SP_001616"
     CDS             198..512
                     /locus_tag="ACF2SP_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFD2359404.1"
                     /translation="MNTLINTSLEVMKNNASKAEQLLKLLANSKRLLILCYLLKQECT
                     VGELEPLVGLSQSALSQHLAKMRREGVLSVRKEGTRVYYFISKPEVEAVLSTLYLIYC
                     KE"
     gene            561..1961
                     /locus_tag="ACF2SP_001617"
     CDS             561..1961
                     /locus_tag="ACF2SP_001617"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2359405.1"
                     /translation="MLLSSITPRKHSAFIIVISALSTSLCAGSVPISYSKAIQLALAN
                     NPKIHAANASIEAAVGASKQAKSMSWPQLDLEVSGARTDNPMSVFGYKLSQGNASFAD
                     FGANQYTGLNTLYTKPQALNHPGYYSNLDTAFKLSVPIYTGGRIKAQQAQTRALVASA
                     QRGNQQAQNQLAYEVYVSYERFLTAKELVGIAERQVARAKVFLSTTKDLKKQSITLNS
                     DVLMAEAYLNSSLLGLSNARIQTEDELDGFRTLLGSPESTFIPSRHTTLSAKTKLNFL
                     LMTRAINDNPSIKTLHARLQAEKAVIAASLALYKPQVSMQLRHDWNGNTIGSGLPSDT
                     IALGANWTLFSAGERAGAVQVASANAKKIQFELDEQRNQLRLQLKQIRRAEQQTLYEL
                     QLSRQTAEKEEAVIQKLKQRFGRGLVPLSALLESQMKLTQSKAQELQALHQSRVHQAH
                     WLMLSNQLISKAIKSA"
     gene            1984..5238
                     /locus_tag="ACF2SP_001618"
     CDS             1984..5238
                     /locus_tag="ACF2SP_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2359406.1"
                     /translation="MVKDVFYSKNKLNKAGQLARTFIQSKLTLLLIVGILLFGVLALE
                     RTPRSYNPEIIVPAVTISVSRPGSDSQEMLHQIVRPLEGLMASISGVEHTYGMAIDDS
                     AMVTVRFKVNENEEASLVKVYNQINSNMDKMPPGTLMPLIRSISLYDVPLVTLTLHAK
                     GANPVDLRTLADKVLEELRTVPQVGKSWVMGASPQAIRVWLNPKHMAEHAISIANIQQ
                     AFSVNNISLDAGTLEHELSETSLRIEGSLISNQDLANIIIGTDGAKPILLKDIATIEK
                     GPQDETIASYFAYGAHGPLKNADIEPAVTIALARQKGSNGVDVAKALLNKLEQLQNSQ
                     LIPKNIDVSITRNYGDEANDAVNTLVEHLGIAILSVVVLLMVFLGWREASVVVFSIPL
                     ILCLVLGIGWLSGQTINRITLFALILSLGLLVDDSIVVIENIHRHIIKGVQKNFTRLV
                     IYAANEIGKPTIIATFTVMLALLPMDFVGGMMGPFMAPIPFNAPLAMIVSLFIAYSVV
                     PYLAYRWLRTKAKKQMNEHHQSHSTWLQRAYLVLFKGLLYSSKKRHLFYLIIVLLLFA
                     VLLQPAWQFIRPAGPNGPLSPLGVEIKMLPDDNVNTFLVSVDAGANATLTKTNELLRE
                     LSATIQKNPYVTDLELYLGESAPEDFAALVRGDNLLRGSQYGQIRVNLVNKHHRAIGS
                     HQIATQVYQGLVHLQKQHPQSHIKLFESPPGPPVRSQMEAGLYGPNYNELRSVANKIT
                     SEVYPKIYGMINIDNSVTQDTNQYRIEINHNAVMLAGLAPKTVAQEVSALFKGISIGN
                     AHEPNARQPEHIILRLPQKAREVAQVLNQLCLKNQQQQLIPLAKIATLHEDKEEKPIF
                     TKDQTPVVYVTGEVLGTSPAYAVTSATNMLKKPPFSMGVENLGFHESQPNRFAKSQLF
                     WLGEMRLTLDVFRDLGSAFIVAIVLIYILLTAFYQSFFIPLIIMGAIPLTVIGVFPGH
                     WLMNQPFTATSMIGVIALAGIVVRNSLLLIDFILEHQHRGSSIVEAVIESGVERILPI
                     VLTALAIILGSAVMLSDPVFGGLAISLIFGSFASTVLTLFLIPLIYLSWWKRNH"
     gene            5257..5457
                     /locus_tag="ACF2SP_001619"
     CDS             5257..5457
                     /locus_tag="ACF2SP_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HFD2359407.1"
                     /translation="MMTIERIIRIVAGAFVLISLALGVSTSPVFINVNFLWFTVFVGA
                     NLLQSGFTGFCPLEMILKKFIG"
     gene            complement(5664..5837)
                     /locus_tag="ACF2SP_001620"
     CDS             complement(5664..5837)
                     /locus_tag="ACF2SP_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359408.1"
                     /translation="MFTNGWFLGSLGIAIIVLIGLIFWFRKTKKTIHFYTALITFLIL
                     AAIFLHGVSFKLN"
     gene            complement(5851..6015)
                     /locus_tag="ACF2SP_001621"
     CDS             complement(5851..6015)
                     /locus_tag="ACF2SP_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359409.1"
                     /translation="MNINGFKIIVLIGLLGLGLTACTPKTTPAPAHPEVGYEGGFGGD
                     GGHNHQPKND"
     gene            complement(6157..6531)
                     /locus_tag="ACF2SP_001622"
     CDS             complement(6157..6531)
                     /locus_tag="ACF2SP_001622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2359410.1"
                     /translation="MMNDWVENEQCSAHPLRFLVVESSLGIRILIRMHLLELKHLVDM
                     AGDDDSARELAFMRVYDFILVDKNLNCHELIDQIQKNSVLNNHTPMIILTSNPNQENL
                     HDSATYLKNLYPRRMQYSCLIL"
     gene            complement(6568..8724)
                     /locus_tag="ACF2SP_001623"
     CDS             complement(6568..8724)
                     /locus_tag="ACF2SP_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD2359411.1"
                     /translation="MTKPLTLTTFFVAGLDCPAEEQLIRRQLKVIPEVAQMDFNFIAE
                     EVTVHHRLPAIDVLQKQIEALGMSVRVQGSTSSAKEEKKQVDSSWPLLALAGLLALCS
                     ELTAYFIAKEQSIWVILPAVLAIILSGPSTFKKGLIALRTKAMNINSLMLIAITGAMI
                     IGAWPEAAMVTVLFALAERIERYSLDKARLAIRSLMQIAPEVARIKMDNGQWQTLPVE
                     DVAVGAVFKVKPGERIPLDGVLISGQSTVNQAPITGESMPVSKQVGDDVFAGTLNEHG
                     AFEVKVTKASGDTLLAKIGKAIEQAQSERAPTQRFVDEFAKYYTPIMVLIALVIALIP
                     PLVFGYPFYDWIYKALTLLVIACPCALVISTPVTVVSGLASAAQHGLLIKGGSYLELG
                     HQLKLIALDKTGTLTEGKPVVTDFIVYDKIQPKEYLLVLAASLDSHSEHPVAHALVEY
                     WKQSQPNGSLFEVEQFSALPGRGVQGIISQELYYVGNHQLAEDNKVCSLAVEQELKRL
                     EEEGKTTVILSNSAVVLAVFAVADTLRSTSKWAIDTLHQQGLKTAMLTGDNALTAKAI
                     AKVVGIDEVQANVLPTEKLQAINHLLEQYKTVGMVGDGINDAPALAKATISFAMGKGT
                     DTALETADVALMNDNLAMLPLYIDLSRKTARILRQNITLSLAIKGVFFILALGGFATL
                     WMAVFADMGASLIVVANGLRLLNSHKGHSEYQEMNG"
     gene            complement(8721..8990)
                     /locus_tag="ACF2SP_001624"
     CDS             complement(8721..8990)
                     /locus_tag="ACF2SP_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFD2359412.1"
                     /translation="MSFLKRMLGSLGGHHGGYRRSHHGQISQQNNYSTNPEPRGLSCP
                     RCKSSLAPGSRFCSQCGSGLENTQCSCGATIAAGANFCGQCGGSL"
     gene            complement(8992..9288)
                     /locus_tag="ACF2SP_001625"
     CDS             complement(8992..9288)
                     /locus_tag="ACF2SP_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFD2359413.1"
                     /translation="MLTQDQIITLSDILHLMGEPNRLKLLIECLHGPKSVSELAEQLQ
                     LSVPLASHHLSLLRSARLLLANREGKHIYYTIYDAHVRCILEDMLKHFTEETEN"
     gene            9423..9629
                     /locus_tag="ACF2SP_001626"
     CDS             9423..9629
                     /locus_tag="ACF2SP_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359414.1"
                     /translation="MTTLLSNHSLLIILGFAFFMGIILIILAKGSSGHKMKFITHLGF
                     YLSLAAALIFAIVLFIDVFPLILK"
     gene            complement(9664..12816)
                     /locus_tag="ACF2SP_001627"
     CDS             complement(9664..12816)
                     /locus_tag="ACF2SP_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2359415.1"
                     /translation="MLEKIIRFSLKHRWFVLLFTTIIALYGVYNFQRLPIDAVPDITN
                     VQVQINTQASGYSPFEVEQRITFPIELAMSGLPNLDYTRSLSRYGLSQVTVVFKDGTN
                     IYFARQLINERLQEVKDKLPPGVETTLGPISTGLGEIFMYTVTNKANVPASQRYNPTE
                     LRTIQDWIIKPQLRNVEGVAEVNTIGGYEKQFHITPDPMKLVRYGLSLNNVVEALERN
                     NANVGAGYIETNGEQNLIRVPGQVQNITDIENIVIASFEGTPIRIRDIAEVALGKELR
                     TGAATENSKEVVLGTVFMLMGENSRTVSERVAAQMKVINKSLPEGIEAVTVYNRTLLV
                     NATINTVKKNLLEGALLVCVILFLFLGNIRAALITAMVIPLSMLLTITGMVSNKISAN
                     LMSLGALDFGLIVDGAVIIVENCIKHLGEQQHALHRVLNLEERLKVIAYATTEVIRPS
                     IFGVFIITVVYLPILTLTGVEGKMFLPMAQTVITALLASMLFALTFVPAAVAIFLGGR
                     VQEKENWLVHYLSLVYAKALRRCFHARRLVIGAAVALVGISLLLAFRLGGEFIPSLDE
                     GDIAMHAMRIPGTSLTQAITMQHLVEERVSQFPEVKRVFAKLGTAEVATDPMPPNVAD
                     TFIMLKPRKDWPNPKKSKQQLVQEIEAAVKQIPGNNYEFTQPIQMRFNELISGVRSDV
                     AVKVFGDDMNTLLEVAEAISAQLKKVPGAADVKVEQVSGLPLLTVEINRDALARYGLQ
                     IGTVQDAIVIATGGKKGGELFEGDKRFDLVVRLPEELRTNPNVLRQVFIPLPQAKDGE
                     RHFIPLSEVAKMVRSESPNQISRESGKRRVVVSANVRNRDLSSFVNEAKQRIDQSVKI
                     PSGYWVTWGGQFEQLQSASQRLQIVVPITLLGIFLLLFMSFGRVRDALLVFSGIPLAL
                     TGGVFALWLRGIPLSISAGVGFIALSGVAVLNGLVMITFINKLHEQKKMYLKDAVLQG
                     SLARLRPVLMTALVASLGFVPMALATGTGSEVQRPLATVVIGGIISSTFLTLLVLPGL
                     YYLFHERHKKSVIKKSGEDE"
     gene            complement(12826..14085)
                     /locus_tag="ACF2SP_001628"
     CDS             complement(12826..14085)
                     /locus_tag="ACF2SP_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2359416.1"
                     /translation="MRVLKPFLSCMPFIILLLGLSSFSLWATNEESHGDEEKNHQETM
                     EKGPQGGRLFKDEDIALEFLIFERGMPPHFRAYLYKNGEPIAPGKAQLTVQLKRFGDK
                     KEAIAFTPVENFLQSVEVIEEPHSFDVSIQLELQDKRYTWHYATYEGRIKMVPAILKA
                     ADIQTAKAQSQTIKTQLKVVGKIAPNRDTLAPIYARYSGIIKSMTKNLGDEVTKGEVL
                     VTIESNESLQNYTINAPMSGTIVQKYATNGELAQSTKPIYEVANLATVWADFTLYRKE
                     VPLVKQGMSVVVTGDEGTPKSISTISYIAPLGIEDSQTTLARAVLSNEPRVWLPGMYI
                     NAAITIKEKTVPVAILLSAIQRMDEKEVIFIQQGDYFEATPVILGQRDDEWVEVVSGL
                     DAGQRYVSNNSFFMKAELGKDGASHEH"
     gene            complement(14082..15329)
                     /locus_tag="ACF2SP_001629"
     CDS             complement(14082..15329)
                     /locus_tag="ACF2SP_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2359417.1"
                     /translation="MRYLFYYVVWSAMSLVASEALAATPLTFKQALGIAYRNNPELQA
                     EIDKAQAMRGFFIQSGLYPNPQVTLTAENFGGSGSYSSYEAAETTASVTQPIPLGNRL
                     QYLKKATYSDYLASLAQIQVQKAILYVAVGVAYVDALYAGQWHQVTQKLIRLNQDIVS
                     AIERRVKAGAGAELDLRLAQVRLGEARIQENKAARDALFQRAKLARLLGDGLRVDGQL
                     VDKGLPGVTLNWAVLLRKLPQSPQLVQLQQQLHAKRATITAVKKSVWPDLNIQLGGRH
                     FSDDGSNAAVASAFAEVPVYNRNQGKIITAEAQYTQTAHELQGTRLDVRQNVYGAFLQ
                     AEQSHYEASLVTNSLLPLARKSIKLAQDGYQMGRYTYIELSTALNSLYEEERHYQQAH
                     ADYHKALIQLTGLLGLAPSKESK"
     gene            15498..16232
                     /locus_tag="ACF2SP_001630"
     CDS             15498..16232
                     /locus_tag="ACF2SP_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2359418.1"
                     /translation="MHLRLLFVFLIILTAELKAQSLTIGIVAYDPPFSMNADKNHFFG
                     FDVELITELCKQIKAQCQLKPMLFDELFTELKASKIDLGIAAIIITDERQKTFLFSLP
                     YLASAARFMTTKNDSITSLKKLNNRSIGIIRGSLFKQLITQKFKTNRVREYPSTNQLI
                     TALNQQEVDALLMDDFSADYWIANNNTLFKGLGKPFPVGIGYGIMAKMDSKMLMNQIN
                     QALKHIEKNGFYLSLYQKYFHTIPMH"
     gene            complement(16285..16458)
                     /locus_tag="ACF2SP_001631"
     CDS             complement(16285..16458)
                     /locus_tag="ACF2SP_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359419.1"
                     /translation="MMKKSSLVTPYVLVLISLMFLLASCASSINKEDSKNNKQPKRVF
                     GGYHPRGHGHGHH"
     gene            16622..17068
                     /locus_tag="ACF2SP_001632"
     CDS             16622..17068
                     /locus_tag="ACF2SP_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFD2359420.1"
                     /translation="MKKEVFISICGGLIFSLSTQTILADEIPSRTLPMSEILKILQTR
                     GYSVIREVEFDDGLYEVEAFDEKGIQLIIRMNPNTGDFINTNLKTKKYPISMLEAAQR
                     IEGAGYHTIFKIEAHGSQYEAKALGTEDKKIKLRIDANSGKIYILE"
BASE COUNT         5127 a         3806 c         3297 g         4940 t
ORIGIN      
        1 ataattacgt gaaatacatc atgaaataat taggatagaa gtcgcaaata agtatgccca
       61 ttattttgag acagatttga agccgtaacc taaacacagt gtgattaagt caagacccgg
      121 ttgctcgact ttaactactc gtaaaaaaag attgtacctg acattgaaat attagaattt
      181 tctaatataa tgaccctatg aatacattaa taaacactag ccttgaagtg atgaaaaata
      241 atgcttctaa ggctgaacaa ttacttaaac ttttagccaa ttccaaaagg cttctcattc
      301 tatgctatct cctcaaacaa gaatgcacgg ttggcgaact agaacccctt gtcggtttgt
      361 ctcaatctgc gctttcacag catttagcta aaatgaggcg cgaaggtgta ctttcggtaa
      421 gaaaagaagg gactcgagtt tattatttta ttagcaaacc cgaagtggaa gctgttttat
      481 caacgcttta tttaatttat tgcaaagaat aaaaatcgct ccattcatta gaatactcta
      541 atgttttaag gatttctgtc atgttattaa gtagtattac cccaagaaaa cacagtgctt
      601 ttattattgt aataagtgca ctgtcgactt ctctttgcgc aggctcggta cccatctcat
      661 attctaaagc gattcagctt gcgctcgcta ataaccctaa aatccatgcg gcaaacgctt
      721 caatagaagc agcggtcggg gcctcaaagc aagcaaaaag tatgagttgg ccacaattag
      781 accttgaggt aagcggagca aggactgata atccgatgtc ggtatttggt tataaactaa
      841 gtcaagggaa tgccagtttt gctgattttg gcgccaatca atatactgga ctaaatacct
      901 tatataccaa gcctcaagca ttaaaccacc caggatatta tagtaatctg gatactgcgt
      961 ttaaactaag cgttcctatt tatactggag gaagaataaa agcgcagcaa gcacagacgc
     1021 gtgctttagt tgcatccgca caacgtggca atcaacaagc tcaaaatcaa ctggcgtatg
     1081 aagtctatgt gtcctatgag cgttttttaa cagccaagga actggtaggc atcgctgaaa
     1141 gacaggttgc tcgagcaaag gtgtttctgt caacaaccaa ggacctaaaa aagcaatcta
     1201 ttaccctaaa tagcgatgta ttaatggcag aggcttactt aaacagctct cttcttggtt
     1261 tatctaatgc aagaattcaa acagaagatg aacttgatgg ctttagaacg ttgcttggta
     1321 gtcccgaaag cacctttata ccaagtcggc acacaacctt gagtgcaaag acaaaactca
     1381 actttttatt gatgacacga gcaatcaatg ataatccatc aattaaaacc cttcatgcac
     1441 gtcttcaagc agaaaaagcg gtaattgctg cctctttggc actctataag ccccaagtgt
     1501 cgatgcaatt aagacatgat tggaatggca ataccatagg ttcaggcctt ccgtcagata
     1561 cgattgcgct aggtgctaac tggactttat ttagtgcggg cgagcgcgct ggcgctgtcc
     1621 aggtagcaag tgcaaatgca aaaaaaattc aatttgagct tgatgagcaa cgaaatcagc
     1681 tgcgattaca attaaagcaa atacggcgtg ccgagcaaca aactctttac gagttgcaat
     1741 taagtcgaca aacagcagag aaagaagagg cagtaattca aaaactaaag cagcgatttg
     1801 gtcgtgggtt ggttcctcta agtgctttat tggagagtca gatgaaacta acgcagagta
     1861 aagcacaaga actgcaagcg cttcatcaat caagagtgca tcaagcccat tggctgatgc
     1921 tcagtaatca actaatttct aaagcgataa aaagcgcata aattgattgt ataccaggag
     1981 actatggtga aggatgtgtt ttattccaaa aataagctca ataaagcagg gcaattggcg
     2041 cgaaccttta ttcaatccaa gctgactctg ttactgattg ttggtattct gttgtttggc
     2101 gtacttgccc tagaacgcac cccgcgaagc tataatcctg aaattattgt tccagcagta
     2161 actatttctg tgagtcgccc tggtagtgat agccaagaaa tgctacatca aattgttcgc
     2221 ccactagaag ggttgatggc ctctatttca ggcgttgagc atacttacgg gatggccatt
     2281 gatgatagtg ctatggttac ggttcgcttt aaagtgaatg aaaatgaaga ggcgagcttg
     2341 gttaaggtct ataaccaaat caacagcaac atggataaaa tgcccccggg tactttaatg
     2401 cctttaatcc gctctatcag tttgtacgat gttccactag tgacactgac attacatgct
     2461 aaaggcgcaa atccagttga cttacgcacg cttgccgata aagtcttgga ggaattgcgc
     2521 accgtacccc aagttgggaa aagttgggta atgggtgcat cacctcaggc tattcgtgtc
     2581 tggctgaacc caaagcacat ggcagagcac gccatttcaa ttgccaacat tcaacaagcc
     2641 ttctctgtta ataacatcag tcttgacgca ggaacattag agcatgaatt aagtgaaaca
     2701 tctcttcgca tcgagggctc actcatttcc aatcaagact tagccaacat tattattgga
     2761 actgatgggg ccaaacctat tttattaaaa gatattgcga ctattgaaaa ggggcctcaa
     2821 gatgaaacta ttgcgtccta ttttgcctat ggagcacacg gcccattgaa gaatgcagat
     2881 atagaacctg cagtaaccat tgctcttgca cgtcaaaaag gaagcaatgg tgttgatgta
     2941 gcaaaagcac ttttgaataa acttgagcaa ttacaaaaca gtcagctcat tccaaaaaat
     3001 atagatgtct caatcactcg caattatgga gatgaagcta atgatgcagt gaatactttg
     3061 gtcgagcact tagggattgc gattctaagt gtcgtggtat tgctgatggt gtttttagga
     3121 tggcgcgaag catcagtggt tgttttttct attcccttaa ttttgtgcct agtacttggt
     3181 attggctggc tatcaggcca gactatcaat cgaattactt tgtttgcgct cattctttca
     3241 ttaggcctct tagtagacga tagcattgtg gtgattgaaa acattcaccg ccatattatt
     3301 aagggagtgc aaaaaaactt tacccgttta gttatttatg cagccaatga aattggtaag
     3361 ccaacgatta ttgctacctt tacagtgatg ctggcgctac tgcctatgga ttttgtaggt
     3421 ggaatgatgg ggccgtttat ggcgcctata ccttttaatg cgccattggc aatgattgta
     3481 tccctcttta ttgcctatag cgtggttcct tatcttgctt atcgttggtt aagaactaaa
     3541 gctaaaaaac aaatgaatga gcatcatcaa agccatagca cctggttgca gcgagcgtac
     3601 cttgtattat tcaaaggatt actttactca tcgaaaaaac gacatctctt ttatttgatt
     3661 attgtactat tgctcttcgc ggtattactc caacctgcct ggcaatttat tcgccctgca
     3721 ggccctaatg gcccattaag cccgttagga gttgaaatta aaatgctacc cgatgacaac
     3781 gtgaatacct ttttagtgtc cgttgatgca ggcgctaacg caacgttaac gaaaacaaac
     3841 gagttacttc gcgaacttag tgcaaccatc caaaaaaatc cttacgtcac agacttggag
     3901 ctgtacttag gagaatcagc cccagaagat tttgccgctc tggtgcgtgg agataatcta
     3961 ttaagaggca gtcaatacgg gcaaatacgc gtgaatttgg tgaacaaaca tcatcgagct
     4021 attggctcac accaaattgc aacacaggtt taccagggac ttgtgcattt gcaaaaacaa
     4081 catccccaaa gccatatcaa gctttttgaa agccctcctg ggccgcctgt tcgctcacaa
     4141 atggaggcag gtctctacgg gcctaattac aatgaattga ggtctgtcgc taataagata
     4201 acctctgaag tgtatcccaa aatatacggc atgataaata ttgataactc agtgactcaa
     4261 gacaccaatc aataccgcat tgaaatcaat cataatgcag tcatgctggc aggcttagcc
     4321 ccaaaaacgg tagcgcagga ggtaagcgct ttgttcaagg gtatttccat cggaaatgca
     4381 catgaaccca atgccagaca gcccgagcac atcatcttac gtcttccgca aaaggcacgt
     4441 gaagttgctc aagtacttaa tcagctgtgt ttaaaaaatc agcaacaaca actaatccct
     4501 ctggcaaaaa ttgcaacctt acatgaagat aaagaggaaa aacccatctt cactaaagat
     4561 caaacacccg ttgtttatgt aaccggtgaa gtgttgggca cgagccctgc ttatgcggtc
     4621 acttctgcaa caaatatgct caagaagccc cctttttcaa tgggggttga aaatttagga
     4681 tttcatgaat cacagcctaa tcgctttgct aaaagtcaac tcttttggct tggtgaaatg
     4741 cgcctgacgt tggatgtttt tcgtgattta ggctcagcct ttattgtggc tatagtcttg
     4801 atttatattt tactaacagc tttttaccag tcgtttttta ttcccctaat tattatgggg
     4861 gctataccgc taactgtcat tggcgttttt cctggccatt ggttaatgaa ccaaccgttc
     4921 actgccacgt ccatgatagg ggtaattgct ttagccggaa ttgttgttcg aaattcgcta
     4981 ttactcatcg attttatttt agaacatcaa catcgaggtt cctctatcgt agaagccgtt
     5041 attgagtcag gcgtggagcg aattctaccg attgtcttaa cagcacttgc tattattctg
     5101 ggctccgctg tgatgttatc cgatcctgtc tttggcggcc ttgctatttc tttgatattt
     5161 ggttcatttg cttcaacagt acttaccttg tttttaattc cattgatcta tttaagttgg
     5221 tggaagcgta accattaatt agtattagga gaatagatga tgactattga acgtattatt
     5281 cgaattgtcg ctggtgcctt tgttttgata tcactcgctc taggcgtgtc gaccagccca
     5341 gtatttatca acgttaattt tttatggttt acggtatttg taggagctaa tcttttgcaa
     5401 agtggattta caggattttg tcctcttgaa atgattttaa agaagtttat tgggtaagag
     5461 ctagtattcg gatataaaaa attaacgaag taaaataaat aggaactagt gttacgagta
     5521 atttaaccca taatgccacc agcgacatcg acaattaaaa agcctcccgt gagcaaaaga
     5581 gcgaaccaaa gtaactgctc ttttgagtat ttttttaatc atgaggtaca tccttactca
     5641 ttattgctcc tcatcaccac cagctaattt aatttaaatg aaacaccatg taagaaaatt
     5701 gcggcaagaa taagaaaagt aattaaagcg gtgtaaaaat gaatggtttt cttagttttt
     5761 ctgaaccaga agataagacc aatcaataca ataattgcaa tacctaaaga acctaaaaac
     5821 catccattag taaacattat gttttcctat ttagtcgttc ttaggttggt ggttatgacc
     5881 tccatcacca ccaaatcctc cctcatagcc aacctcagga tgagctggag caggtgttgt
     5941 tttaggagtg caggcagtta agcccagacc caataaaccg atcaatacaa ttattttaaa
     6001 tccattaata ttcattattt ctccttaaca gccaatcaaa ataacatgaa actacttaca
     6061 tcgtaatata ctcaataaca taagataaat gcatcttatc ttctccaatt ttacgtttac
     6121 acgccacctt taaaaaaatt cattttttaa tattctttac aaaatcaagc aactgtactg
     6181 catccttctt ggatataggt tttttaaata agtagcgcta tcgtgcaggt tttcctgatt
     6241 agggttggaa gttaaaatga tcataggcgt atggttgttt aatactgaat ttttttgtat
     6301 ctggtcaatc agctcatggc aatttaaatt tttatccact aaaataaaat cataaactct
     6361 catgaaagcc aactctcttg ccgagtcatc atctcctgcc atatccacca aatgcttcaa
     6421 ctctaataaa tgcatacgaa tgagaatacg aattcctaat gaggattcca ccactaaaaa
     6481 acgcaatgga tgagcgctgc attgttcatt ttcaacccaa tcattcatca tgcaacctct
     6541 ttcgtgacaa acccgcaaag actctcatta tccattcatt tcctgatatt cagaatgccc
     6601 tttatgggaa ttaagtaagc gcagaccatt cgcaacaaca attagactcg cacccatatc
     6661 agcaaatacc gccatccata gcgttgcgaa tcctcccaag gccagaataa aaaacacccc
     6721 tttgatggca agggacaaag taatattttg gcgaagaata cgcgccgttt ttcggcttaa
     6781 atcaatgtat aaaggcagca tggctaaatt atcattcatt aatgcaacat ctgccgtttc
     6841 taaagcggta tcagtacctt tccccattgc aaaactaatc gtagccttag ccaaggctgg
     6901 agcgtcattg attccatccc ccaccattcc taccgtttta tactgttcca aaaggtgatt
     6961 aattgcttgt aatttttcag taggcaagac attcgcctgc acttcatcaa tccccaccac
     7021 tttggcaatg gctttagctg ttaaggcatt atcacccgtt aacatcgctg ttttaagtcc
     7081 ttgttgatgc aaggtgtcaa ttgcccattt gcttgtagag cgcaaagtat cagccaccgc
     7141 aaaaaccgcc aaaactaccg ctgaattact caaaatgact gttgtttttc cttcctcttc
     7201 caaacgctta agttcctgct ctacagcaag agagcacacc ttattatctt ctgccagttg
     7261 atgatttcca acgtaataca gttcttgact aataatgcct tgcaccccgc ggccaggaag
     7321 tgctgaaaat tgctctacct caaataaaga cccattaggt tggctttgct tccaatactc
     7381 aaccaaagca tgagccactg gatgttccga atgactatca aggcttgcag ccagcactaa
     7441 caagtattct tttggttgga ttttatcgta aacgataaaa tcagtcacca caggcttccc
     7501 ctcagttaag gtgcctgttt tatccagggc aattaatttg agctggtgcc ccagttctaa
     7561 atagctgccg cctttgataa gaagaccatg ttgtgctgct gaagccaatc cgctgactac
     7621 cgttaccggt gtagagatga ccaaagcaca agggcaagca ataaccaata acgttaaggc
     7681 cttataaatc caatcataaa aaggataacc aaacaccaac gggggaataa gtgcaataac
     7741 cagtgcaatc aagaccataa ttggggtgta gtatttggca aactcatcga caaagcgttg
     7801 cgttggtgcc cgctcacttt gtgcctgctc aatggcttta ccaattttag caagcaacgt
     7861 atcacccgaa gccttagtga ctttaacttc aaaggcaccg tgctcattta aggttccggc
     7921 aaacacgtca tcacctactt gcttgctcac cggcatggat tcccctgtaa tcggggcttg
     7981 attgaccgta ctttgtcccg atatcaacac cccatccagt gggatgcgct caccgggttt
     8041 tactttaaat accgcaccga ccgctacatc ttctacaggc aaagtctgcc attgaccatt
     8101 atccatttta atccgagcca cctctggtgc aatttgcatg agactgcgaa tggcaaggcg
     8161 ggctttatct aatgaataac gttcgattcg ctcagcaaga gcaaataaaa cggtgaccat
     8221 cgcagcttcc ggccaggcac ctataatcat agccccggtg attgcaatta acatcaggct
     8281 attaatattc atggccttag tacgaagcgc gattaaaccc ttcttaaaag tagaagggcc
     8341 acttaagata atggcaagca ccgcaggcaa aatgacccag attgattgct ctttggcaat
     8401 gaaataagca gttaactcag agcaaagcgc taaaagacct gccaaagcaa gcaatggcca
     8461 tgacgaatca acttgcttct tctcttcttt tgcagaggag gtactgcctt gaacccgaac
     8521 actcatgcct aacgcttcaa tttgtttttg caacacatca atagctggaa gacgatggtg
     8581 cacggtcact tcctcagcaa tgaaattaaa atccatctgc gccacttcag gaatcacttt
     8641 taattgccgc ctgattaatt gctcttcagc cgggcaatca aggcctgcaa caaaaaaagt
     8701 ggttaaggtt agaggctttg tcataatgag cccccacatt ggccgcaaaa atttgcccct
     8761 gcagcaatag tggcgccaca actgcattga gtattctcaa gaccactgcc acattgacta
     8821 caaaatcgtg atccaggagc tagagagctc ttacagcgcg ggcaactcaa tcctcttggc
     8881 tcaggattgg ttgagtaatt attctgttgg cttatttggc catgatgaga gcgtcggtat
     8941 ccgccatgat ggccgccaag acttccaagc attcgtttta gaaaactcat gttaattctc
     9001 cgtctcttca gtaaagtgtt tgagcatgtc ctctaaaata caacgcacat gcgcatcata
     9061 aatagtgtaa taaatatgct ttccctctct atttgctaga agaaggcgtg cagaacgtaa
     9121 cagacttaaa tgatggctcg ccaatggcac cgaaagttgc agttgttccg ccagctctga
     9181 aactgatttt ggaccatgca aacactcgat aagaagcttt aaacgattag gctcacccat
     9241 caaatgcaag atatcgctta aagtgatgat ttggtcttga gttagcatag tttattaaac
     9301 agttaaataa gtatttaact ttaagtgtag ctcattcaca gcggttgtca aaaacactat
     9361 ccgttgtcct ttgctcaaaa gagctataat taaatgaatc tatttttgta taaggaagaa
     9421 gaatgacgac ccttctttca aatcacagtc ttctgatcat cttaggattt gcttttttta
     9481 tgggaataat tttaattatt ctggcaaaag ggagttccgg ccataaaatg aaatttataa
     9541 ctcacttggg attttatctt tctcttgctg ccgcacttat ctttgctata gtgcttttta
     9601 tagatgtctt tccacttatt ttaaaatgac aatcaccaat atgttacaat tccatcaaag
     9661 gaatcactca tcctcgcctg attttttaat tactgatttc ttgtgccgct catgaaacag
     9721 gtaatacaaa ccgggtaaga ctaagagtgt taggaaagtg gatgagataa tgccacctat
     9781 aaccaccgtt gccaagggac gttgtacttc agagcccgtg cccgtagcca aagccatcgg
     9841 cacaaacccc aaagaggcca ctaaagcagt catgagtacc ggacgaagcc tcgccagaga
     9901 gccttgtaat accgcatctt ttaagtacat ttttttctgt tcatgcagtt tattaataaa
     9961 ggtaatcatg accaggccat taagaactgc cacccctgac aacgcaataa acccaacacc
    10021 ggctgaaatg gacaagggaa tccctcgaag ccacagtgca aataccccac cagttaacgc
    10081 caaaggaatg ccactaaaca ctaagagcgc atctcgtact cgaccaaaac tcataaacaa
    10141 caataagaaa atgcccaata gtgtgatagg aacaacgatt tgtagccgtt gagaggctga
    10201 ttgcaactgc tcaaactgac caccccaggt tacccagtag ccactgggaa ttttgacact
    10261 ctggtcaatc cgttgcttgg cttcattcac aaacgagctt aaatcccgat tgcgaacgtt
    10321 agcactgacc accacacgcc gtttaccact ttcccgactg atttgattgg ggctttcact
    10381 acgcaccatt ttggctactt cactcaacgg aataaaatgt cgttcgccat ctttagcctg
    10441 tggcaacgga ataaatactt ggcgtagtac attagggttt gtacgtaact cttcaggcaa
    10501 gcgcaccact aaatcaaaac gcttatctcc ctcaaagagc tcgccacctt ttttgccgcc
    10561 tgtggcaatg acaatggcat cttgaaccgt gccaatttgt aagccgtagc gtgccaaagc
    10621 atcgcgattg atttcaacgg ttagtagcgg taaaccactg acttgctcta cttttacatc
    10681 agcagcaccg ggcacttttt ttaattgagc actgatggct tcagctactt ccagcaatgt
    10741 attcatgtca tcgccaaata ctttcaccgc aacatcacta cgaacccctg aaattaattc
    10801 attaaagcgc atctgaatgg gctgagtaaa ttcatagtta ttccccggaa tttgctttac
    10861 tgccgcctcg atttcttgta ctagttgctg tttgcttttt ttaggattcg gccaatcctt
    10921 ccgaggcttt aacataataa acgtgtcagc gacattgggc ggcatgggat cagtagccac
    10981 ttccgcagtc cctagttttg caaaaacacg tttgacttct ggaaactggc ttactcgctc
    11041 ctctactaaa tgctgcatgg taatggcttg tgttaaacta gtgccaggaa ttcgcatcgc
    11101 gtgcatggca atatcgcctt catcaagact tggaataaat tcaccaccca accgaaatgc
    11161 aagcaaaaga cttatcccca ccaaagcaac cgcagctcct atcaccaacc ttctggcatg
    11221 aaaacagcgg cgcaaggctt ttgcataaac aagtgagagg tagtgtacca accaattttc
    11281 tttttcttgt acacgcccac ctaaaaaaat ggcaacggct gctggaacaa aagtcagagc
    11341 aaaaagcatg gaggcgagca gcgcggtaat aaccgtttgc gccatcggca aaaacatttt
    11401 cccctcaacc cctgttaacg ttaaaatcgg taaataaacc accgtgataa taaagacacc
    11461 aaagatactg ggccgaatca cctctgttgt ggcataagct atgactttca agcgctcttc
    11521 taaatttaaa acacgatgca aggcatgctg ttgttcaccc aaatgcttaa tgcagttttc
    11581 aacaataatg accgcaccat cgacaatcaa accaaaatca agagcgccta agctcatcaa
    11641 attggcactg attttattag ataccatccc tgtaatggtc aagagcatgg ataaaggaat
    11701 caccatcgcg gtaatcagtg ctgcgcgaat gttacctaaa aacaaaaaca aaataacaca
    11761 aacaagcaat gccccttcga gcaagttttt cttcaccgta ttaatggtag cgttcacaag
    11821 caaggttcgg ttataaaccg tgactgcttc aatgccttcc ggcaatgatt tattaataac
    11881 tttcatttgg gccgcaactc gttcagacac tgtgcgactg ttctcgccca taagcataaa
    11941 tactgtgcct aaaacgactt ctttactgtt ctctgttgct gctcctgttc gtagctcctt
    12001 gcctaaagcc acttctgcaa tatcacgaat tcgaataggt gttccttcaa agcttgcaat
    12061 aacaatgttc tcaatatcag tgatgttttg cacctgccct ggtacgcgaa ttaagttctg
    12121 ctcgccatta gtttcaatat atcctgcacc cacattggca ttattgcgtt ctaatgcctc
    12181 aaccacattg tttaagctta acccataacg aaccagtttc atgggatctg gagtgatgtg
    12241 aaattgtttt tcataacccc caatggtgtt tacttcagcc actccctcta cattacgcag
    12301 ttgcggcttg ataatccaat cttgaatggt acgcagttcc gttggattgt aacgttgact
    12361 tgctggtaca ttggctttat tggttaccgt gtacataaaa atctcaccaa gccctgtaga
    12421 gattgggcct aaagtggttt ctactcctgg tgggagcttg tcttttacct cctgcaagcg
    12481 ctcattaatt aattgacgcg caaaataaat gttcgtgcca tctttaaaga ccaccgtgac
    12541 ttgcgatagt ccataacgtg ataaagaacg agtgtaatcc agattaggca acccgctcat
    12601 agccagttca atagggaaag taattcgttg ctccacctca aagggtgaat agcctgaggc
    12661 ttgagtattg atttgcactt gcacgttggt gatgtcaggt accgcatcaa taggcaaacg
    12721 ttgaaaatta tacacaccat aaagagcaat gattgtcgta aataataaga caaaccatcg
    12781 atgttttagg gaaaaacgaa ttattttttc aagcattacc aatccttagt gctcgtggct
    12841 tgcgccatcc tttcctaact ctgctttcat aaaaaagcta ttattgctga catagcgctg
    12901 gcctgcatca agccccgaaa ccacttcgac ccattcatca tccctttggc ctaaaataac
    12961 aggcgttgcc tcaaaataat ctccttgctg gataaaaatt acttctttct catccatgcg
    13021 ctgaatagcc gacaaaagta tggcaactgg aaccgttttt tctttaatgg taattgcggc
    13081 attaatgtac atcccaggca accaaacacg aggctcatta gaaagaactg ctcgtgcgag
    13141 cgttgtttga ctgtcttcaa tgcctaaggg cgcaatgtag gagatggtgc tgatggattt
    13201 tggtgttcct tcatctccag ttaccaccac actcattcct tgttttacta aaggcacctc
    13261 tttacgatat aaggtaaaat cagcccacac agtagccaaa ttagccactt cataaatagg
    13321 tttggtgctt tgcgcaagct ccccattggt tgcgtatttt tgcacgatgg ttccgctcat
    13381 gggtgcatta attgtataat tttgtaaact ttcgttactc tcaatcgtaa ctaatacctc
    13441 ccctttggtg acttcatcgc ctaaattctt agtcattgat ttaataatgc ctgaatagcg
    13501 tgcatagatg ggagctaagg tgtctcgatt gggagcaatt ttacctacga ctttaagttg
    13561 agttttaatt gtttggcttt gtgccttagc cgtttgaatg tctgccgctt ttagaatggc
    13621 tggcaccatt ttaatgcggc cttcataagt cgcataatgc caggtatagc gtttatcctg
    13681 aagctctaat tgaatgctca catcaaaaga atgaggctct tcaatcacct caacgctttg
    13741 taaaaaattc tcgacaggag tgaacgcaat tgcttctttt ttatcaccaa atcgctttaa
    13801 ctgaacggtc aattgcgctt tcccaggcgc gataggctca ccattcttat acaaataagc
    13861 gcgaaaatgc ggtggcatgc cccgttcaaa aattaaaaac tcaagggcta tatcttcatc
    13921 cttaaacaaa cgcccacctt gcggaccctt ttccatggtt tcttgatgat ttttttcttc
    13981 atcgccatgg gattcttcat ttgttgccca taacgaaaag gaactcaacc ccaataacag
    14041 aattataaag ggcatgcagg ataggaatgg ctttaaaact ctcatttact ctccttagaa
    14101 ggcgctaatc ctaaaagccc ggtcagttga attaaggctt tatgataatc agcatgagct
    14161 tgttggtaat gacgctcttc ttcatacaac gaattaaggg ctgtggataa ttcaatatac
    14221 gtataacgtc ccatttggta gccatcctgt gccaatttga ttgatttacg cgccaaaggt
    14281 aataacgaat tcgtcaccaa gctcgcctca taatgacttt gttcggcttg tagaaaagcc
    14341 ccatagacat tttgacgcac atcaaggcgt gtaccttgaa gctcatgagc cgtttgggtg
    14401 tattgtgcct cagcggttat gatttttcct tgatttctat tgtaaacagg gacttctgca
    14461 aaggctgaag ccactgcggc attactgcca tcatcagaga aatgacgacc gcctaattga
    14521 atgtttaaat ccggccacac agatttttta actgcagtaa tagtcgcacg cttggcgtgt
    14581 aattgctgtt gaagctgtac taactgaggg ctttgcggta attttctaag taacacagcc
    14641 cagttcaatg tcactccagg aagaccttta tccactaatt gtccatcaac tcgtaatcca
    14701 tcacctaata gacgcgccaa tttcgcacgc tgaaataatg catcccttgc agctttattt
    14761 tcttgaatac gcgcttcgcc taagcgcacc tgggccaaac gtaagtctaa ttccgctcca
    14821 gcgcctgcct taactcgacg ctcgattgcg cttacgatgt cttgattgag gcgaataagt
    14881 ttttgggtga cttgatgcca ttgacccgca taaagtgcat ccacataggc cacaccaact
    14941 gccacataaa gtatcgcttt ttgtacttga atttgggcaa gagaggctaa gtaatctgaa
    15001 taggttgctt ttttcagata ctgcaaccga ttacccaaag ggatgggctg ggtcactgat
    15061 gccgtagttt ctgccgcctc ataacttgaa taactccccg agccgccaaa gttttctgcg
    15121 gttaaagtca cttgaggatt gggataaagc ccactttgga taaaaaaacc gcgcatggct
    15181 tgtgctttat ctatttcggc ctgtaattcg ggattattgc gataagcaat gcccagggct
    15241 tgtttgaatg tcaaaggggt ggccgcgagt gcctcactcg ctacaaggct cattgcactc
    15301 cacacaacat aataaaataa ataacgcatt ccctacccta tcaaatcctt gtcaaacctt
    15361 tcgttccagt gtaccttgat tttgggcctt gatacacctt attgctaaaa aaaccagaaa
    15421 atcatagcat taagtcactg aaatgattac caaactggta tacactgatc tttataatcc
    15481 attaacttgt ttatcctatg cacctacgat tattatttgt ttttttaata atcttgactg
    15541 ccgaacttaa ggctcagtca ttaactattg gaatagtagc gtatgacccg ccgttttcaa
    15601 tgaatgccga caaaaatcat ttctttggat ttgatgttga gctcataaca gagctttgca
    15661 agcaaattaa agctcagtgt caattgaaac ccatgctgtt tgacgagctg tttacagaat
    15721 taaaagcgag taaaattgac ttggggatag cggcaattat catcaccgac gaacgacaga
    15781 aaacctttct ctttagtctt ccctatctgg caagtgcagc acgttttatg accacaaaaa
    15841 acgactcaat tacatctctt aaaaaactca acaatagatc aataggcatt atcagaggct
    15901 ctctcttcaa acaactcatt actcagaaat ttaaaaccaa tagggttaga gaatatcctt
    15961 caactaatca actcatcact gccttaaatc aacaggaagt ggatgccttg cttatggatg
    16021 acttttcggc tgattattgg atagccaata ataacacctt gtttaaagga ttaggtaaac
    16081 ccttccccgt cggcatcggt tatggaatca tggccaaaat ggattctaag atgctaatga
    16141 accaaatcaa tcaagcctta aaacatatcg aaaaaaatgg attttattta tctttatatc
    16201 aaaaatattt tcacaccata cccatgcatt aaaatatgaa tcactaacgg ttatcttaat
    16261 gtcaattttt taatttaatt cggtttagtg gtgtccgtga ccatgaccac gcgggtgata
    16321 cccaccaaat acacgtttag gctgtttatt gttttttgaa tcttccttat taatagaaga
    16381 agcgcaggaa gccaacaaaa acattaaaga aatgagcact aatacatatg gggttaccaa
    16441 actagatttt ttcatcattc gcatccttgg aataactcac ttgctgttat tttttaatat
    16501 cattattatg gatgaaataa aagtaaattg tactcctttg gttttatatt cattcaatat
    16561 aaaaaaaacg gagttaaatt gatgaaattt tattcccgtc aacaccgcac aggaggcgaa
    16621 tatgaaaaaa gaggttttta tttcaatctg tggcggcctt attttttcgt tatccacaca
    16681 aacaatcctg gccgatgaaa ttcccagtcg caccttacct atgtctgaaa tcttgaaaat
    16741 tctacagact cgcggttata gcgtaataag agaagttgaa tttgatgatg gtttatatga
    16801 ggtggaagcc tttgatgaaa aagggattca gcttattatt cgaatgaacc caaatacagg
    16861 ggactttatc aacaccaatt taaaaaccaa gaaatacccc atctcaatgc ttgaagcggc
    16921 gcagagaatt gagggcgctg gctatcatac tattttcaaa attgaagcgc acggcagtca
    16981 gtatgaggca aaggcccttg gtacagagga taaaaaaatt aaattacgca tcgatgctaa
    17041 ttctggcaaa atatatatcc ttgaataaga gaactagagt tattaattct gcaacattta
    17101 aaatttttca ataaataaaa cagccaacct atttaagtta agcttaaaga aatttaagac
    17161 ttgacatcct
//
LOCUS       DAXLDT010000062        16905 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXLDT010000062 DAXLDT010000000
VERSION     DAXLDT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.062"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..5115)
                     /gene="sidH"
                     /locus_tag="ACF2SP_001633"
     CDS             complement(<1..5115)
                     /gene="sidH"
                     /locus_tag="ACF2SP_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidH"
                     /protein_id="HFD2359421.1"
                     /translation="MKRTIETYIIYLKEDLKIADTCKTIKDGLLKSITDKTHFSEELA
                     AYFERDNPNVPFKVNTTDPTQVAVLKKLLNALENAEKSFRAIENIDISRDRYTAMIAK
                     DAVMVSYKAVHEIYAALQLINHSNSDIQDIVGPHIQKLLPQMALASKALGNFAPEHPE
                     ESAGAVLAGVVNMLPTEKPTESESLGKLSNLIFELPHYFEELQKLIATGASGIATKSI
                     TSAEDYQSAMIKKANETKYYFEQLSSKSGLSAIPSYLSIVKRLIAHSTDLVNAGAPLT
                     KQAYLDAVAKLEDIKHNILPQLISELEMVEESMGLKPGLLTDPALEQMNKYYTQLAEQ
                     VDNIAKAAGVLDTVSDYSDSIGGKIVRFLAGDSKKLDVGPKLTSAPDLGVLMDDVFIQ
                     KRRSNQESRLNEARLGSEDKNVLAAANRFFDKIGSYNSIHKAWSKWSLANISQSEKDA
                     LIKEYKQFQPHFAALYPDIDKLVVDALTQPTGSNIVSRLYSSDYKQLWSSDHFKQVLS
                     CKDSVLSSIQQSLAQSEFKAKLIEKTMSHSEETAYTMNNKTTNLTTRVQPFEPLKFTL
                     EDDKPVEYYHKKVIAASNQILELERAQKGVAEFFNYIQKKYPHENPSFDSLDESDKEF
                     LRKAYKTFQPQLLALKHDDINTRLVSSLTSSKPTDPPLRLTDLVSLKSGIDDYLNEKI
                     SDLNQDKTTLLDKEEEAREEQYAKAPLVARGAELEKQTLFGQMSKLKLSKSVDDFFNK
                     KFQTYLKDNLSPEIWKQLSSNGETLDFDKIPYLEFHKDSPEVAMYKQLINSIHYMKNG
                     LENLESLNDYGDPNNIYHRTRFVMTTFNALVMNICFSKYYVMEAGNNPGLKAIVQEGL
                     DLLKPLEGMPLIGDYLKTTEKQEPPKQNIITAWKKQQAVVESGLSKGPKPKTDQQLIS
                     EQLGKIQEAIDNFDGDLEISDSAREKIKTQIGEFAKGISGLSFGPGSAKKILAALTKL
                     ETQLSNLDKESPEVTLGKLKDTHSELNAQFRAAAEYTEYHSGQKFGSYSNNISTIVSN
                     FCNGLVSNLPLKQEPAPKGKTPEKSVTPVITGATNPHEVVFGTEHEEFNSVYQPYLLL
                     KRITDEFRDQNNPYKPSFDELKEEAVSYYDKIQPLLESVDPKFDKNFIAKHKESSTLL
                     KAIDEVMSMRQRINNPESSFAKLKDLHLEGDFEKEENKEKFRQLYKEIQPYLHKIDST
                     YDQTQFLEGLKTAKDFSGALQRIMNEENALQQSTSLKDTSYLQLIAESLYQIPVKLNK
                     LKAEPDTPEPSKEEIDANVKAFVEGLNGLSFGPGSVKKILSTAAKLQMQLSDIGKEGR
                     ELTMGRLKEIQAEFGTILMAAADNAEFHLGLKPGTYSRTVSERFEKFYSSLIVNLPLE
                     KDQTGLELLIDTTSTQKRLAREMERLERVKEDTSAIDTKKSIFGTEHEQFSTLYQPYA
                     SLRHIAKDIEDGVHMNLYERTLEELKEEASNEYKKIQPYLAKINPEFTEDYISKTDGE
                     YSLLHAIDRVFEERHKINKPSSPFDKLRDLYLDGDFEKEENKEQFLQLYAELQPHLIK
                     INYQYDLAYFLRELQTPEDFKAATERIINDESKLQELITGLDDTKRLKVKLCEERIGY
                     FIDLLKKQELEVGPEKIQAFKEKIFFNYIHANVNSALEAKIGSHAEQFLQFIEKDFLD
                     KKNEILEKITIDQDMEEEIAKAIDRIAPDIINNKIES"
     gene            complement(5236..5976)
                     /gene="lubX"
                     /locus_tag="ACF2SP_001634"
                     /gene_synonym="legU2"
     CDS             complement(5236..5976)
                     /gene="lubX"
                     /locus_tag="ACF2SP_001634"
                     /gene_synonym="legU2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector E3 ubiquitin ligase
                     LubX/LegU2"
                     /protein_id="HFD2359422.1"
                     /translation="MGYRIEMATRNPFDIDHKSKYLREAALEANLSHPETTPTMLTCP
                     IDSGFLKDPVITPEGFVYNKSSILKWLETKKEDPQSRKPLTAKDLQPFPELLIIVNRF
                     VETQTNYEKLKNRLVQNARVAARQKEYTEIPDIFLCPISKTLIKTPVITAQGKVYDQE
                     ALSNFLIATGNKDETGKKLSIDDVVVFDELYQQIKVYNFYRKREVQKNQIQPSVSNGF
                     GFFSLNFLTSWLWGTEEKKEKTSSDMTY"
     gene            6228..8093
                     /gene="vipD"
                     /locus_tag="ACF2SP_001635"
     CDS             6228..8093
                     /gene="vipD"
                     /locus_tag="ACF2SP_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VipD"
                     /protein_id="HFD2359423.1"
                     /translation="MRLAVIMTKSRKLKRNLLEISKTETGQYSVSAPEHKGLVLSGGG
                     AKGISYLGMIQALQERGKIKNLTHVSGASAGAMTASILAVGMDIKDIKKLIEGLDITK
                     LLDNSGVGFRARGDRFRNILDVIYMMQMKKHLESVQQPIPPEQQMNYGILKQKIALYE
                     DKLSRAGIVINNVDDIINLTKSVKDLEKLDKALNSIPTELKGAKGEQLENPRITLGDL
                     GRLRELLPEENKHLIKNLSVVVTNQTKHELERYSEDSTPQQSIAQVVQWSGAHPVLFV
                     PGRNAKGEYIADGGILDNMPEIEGLDREEVLCVKAEAGTAFEARVNKAKQSAMEAISW
                     FKARMDSLVEATIGGKWLHATSSVLNREKVYYNIDNMIYINTGEVTTTNTSPTPEQRA
                     RAVKNGYDQTMQLLDSHKQTFDHPLMAILYIGHDKLKDALIDEKSEKEIFEASAHAQA
                     ILHLQEQIVKEMNDGDYSSVQNYLDQIEDILTVDAKMDDIQQEKAFALCIKQVNFLSE
                     GKLETYLNKVEAEAKAAAEPSWATKILNLLWAPIEWVVSLFKGPAQDFKVEVQTEPVK
                     VSTSENQETVSNVKDINPAVEYRKIMAEGRREHTDPSPSLQEKENVEPSVTFGNT"
     gene            complement(8150..9790)
                     /locus_tag="ACF2SP_001636"
     CDS             complement(8150..9790)
                     /locus_tag="ACF2SP_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2832 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359424.1"
                     /translation="MTNKTQELQRVIADMAVLIEQDAEPDPQLYAFFFQHPEISLELI
                     HLINNLSEELVHDGPPIYSACVFALDICIAQLQAAAEANNKNATKLLSQLMNHLAEVI
                     DTHKHSLSFWLPVLNAFYEVHVELTDDLKEAYYGLASQDDDAVEVDEQFNHLDSIKDL
                     IHELSDLTLFDIAESFFAQSYAMPPDFFADLIVDLYSIEEGKDIALLVLLHPNIEVRE
                     IALSTIDQLMNTITLTPASLSRLQIIKHWYPTHYHGMFDRWIKTQRKKGVTFETEASQ
                     AKITIKATEVDGSGSQGVFIHARKNKKNRLCGLLLKYDLGIKDAWITPEISAAEVTDY
                     YHKAFEDSVTLREVDFSYFQTIVEHFLAITVEKSDIPNLHFLEILEFLGIRLKPDKLD
                     LDCVFEQLSVQIIPFTQEKIAESLKRSKMWLKNKQFTESWYIENPTIDKIVNHNSSFV
                     EGVKVCRLADAMDAVFSEEMELHRDKWQFHFLWIALWVKAKHRKNEKVWQDSFLIAYA
                     IHQGYPLKDIPVMKEICYQTVVNSIETMQERRTHLSQE"
     gene            complement(9787..10167)
                     /locus_tag="ACF2SP_001637"
     CDS             complement(9787..10167)
                     /locus_tag="ACF2SP_001637"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFD2359425.1"
                     /translation="MQTPKGELTIQTLAMPLNTNANGDIFGGWIVSQMDLAAGVLAKR
                     ISHGRVATVAINSMTFLKPVHVGDVVSCHVELVKVGNTSMTIRVEVWAYSSIKMEKYQ
                     VTEGIFVFVAIDENGKPRQVPKTQ"
     gene            complement(10170..12539)
                     /locus_tag="ACF2SP_001638"
     CDS             complement(10170..12539)
                     /locus_tag="ACF2SP_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFD2359426.1"
                     /translation="MAEVMLASAAIIADELKVKVSQVETAIRLLDEGATVPFIARYRK
                     EATGGLDDVQLRFLAERLIYLRELDERRTVILQSIREQEKLTPELEQSILAAETKTRL
                     EDLYLPYRPKRRTKAQIAIEAGLEPLAHALWKDPALDPEEQASQFINSEAGIENTQAA
                     LDGARHILMEHFAEDAELINELREYLWQHGIVKSVGNSTKKDQANKFSDYFDYSEAIK
                     KIPSHRALALFRGRREGILQVSLVLPDQDASYGENKVAAYFNITDKQRKADGWLIDTV
                     RMTWKIKLFTKLELELLTRLREIADEEAIKVFSKNLRDLLLAAPAGPQVTIGLDPGIR
                     TGVKVVVVDATGKLLDYSVIFPFAPQNEWHQSIADLAKLAAKYQVNLISIGNGTGSRE
                     TERLVADLIKMYPDLKLTKIIVSEAGASVYSASEIAANEFPDLDVSLRGAVSIARRLQ
                     DPLAELVKIEAKSIGVGQYQHDVNQAKLARCLDGVVEDCVNAVGVDINTASAALLSHI
                     SGLNDTLAKNIVQFRDEHGAFTNRNQLKNINRMGEKAFQQAAGFLRIMNGDNALDASC
                     VHPEAYPLVEKIISDKKVSIKEIIGNRELLQSINAEQYVDENFGLPTIRDVLRELEKP
                     GRDPRPEFRTAQFKEGVEDIKDLEQGMVLEGVVSNVTNFGAFVDIGVHQDGLVHISAM
                     TNRFITDPRSVVKAGDIVKVKVVEVDKERRRIGLSMKLNDEQAPAVLQKANKPQPARK
                     QAEQKQPEFKKKQEHKKKPDKIKKSEPVKKTVFNTAMADALAKLKRGGE"
     gene            complement(12663..13328)
                     /locus_tag="ACF2SP_001639"
     CDS             complement(12663..13328)
                     /locus_tag="ACF2SP_001639"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HFD2359427.1"
                     /translation="MNKGQYFWNELWCEGRISFHKEEVNPDLIAYVSSLNIPAKGRVL
                     VPLCGKSVDMLWLVRQGYHVVGIELVEKAILQFVQEHQITVRENTIGQAKQYFTDNLN
                     LWVTDIFALNSALIEPVDAIYDRAALVALPKKLRPAYVDICLKWLKPGGSILLKTLQY
                     NQEKVQGPPYSVSPEEIALSYQQCAKIELLKSQKRIQEPNDHLFNLGISEVNDYVWCI
                     RKG"
     gene            complement(13325..15139)
                     /gene="glmS"
                     /locus_tag="ACF2SP_001640"
     CDS             complement(13325..15139)
                     /gene="glmS"
                     /locus_tag="ACF2SP_001640"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFD2359428.1"
                     /translation="MCGIMGAVSERDISKILLEGLRRLEYRGYDSAGIAVIDSQDRLK
                     RVRIQGKVQNLADAMQETAIAGNTGIAHTRWATHGKPSEQNAHPHLSHGEIALVHNGI
                     IENHEHLRQQLITYGYQFTSETDTEVAAHLIHYHYQQHENLLLAVQKAAAEMQGAFAL
                     GVIHQKRPEELVAIRKGSPLVLGFGIGENFIASDALALRSFAQSVIYMEEGDSACVTT
                     QDIKVYDSNRILVQRAVHPLNSDSEIVNKGPYRHFMLKEIFEQSKVITDTLESRINSI
                     DVLRASFGEKASHIFPMVKNIHIVACGTSYHAGMIAKYWLESLAGLPTQVEIASEYRY
                     RDVVVPDNTLFITVSQSGETADTLAALFKAKQSNYLASLAICNVATSTLVREADCVFL
                     TRAGIEIGVASTKAFTTQLAAFLMLAAALCKDNRAQEVLRQLQELPACCERVLQMNEE
                     VESLASLFVNKVHALFLGRGVQYPVALEGALKLKEISYIHAEAYPAGELKHGPLALVD
                     KDMPVIAVAPNDELLDKLKSNLHEVSARGGQLFVFVDDSQNWKANGARLIKVPSCGAW
                     LAPIVYTIPLQLLAYHVAVAKGTDVDQPRNLAKSVTVE"
     gene            complement(15504..16805)
                     /gene="plaC"
                     /locus_tag="ACF2SP_001641"
     CDS             complement(15504..16805)
                     /gene="plaC"
                     /locus_tag="ACF2SP_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase/glycerophospholipid:cholestero
                     l acyltransferase PlaC"
                     /protein_id="HFD2359429.1"
                     /translation="MIQNNRICRWLFIVLLSLPLVGFTSIPVKSIVVFGDSLSDNGNT
                     THLLKSLRQEEDPAFLVAPFKIFVINKMIEFANDYYVPQMVLDAGISAVTDFFDHDLA
                     PYLANLVGKVKQVPVLPGKPYWKNRFSDGRVWIEYLAEMLSIQKSDEEVYLNKAFGGS
                     WSATYDYQLTVWNLIRHPLGTIKNLIVGKLIPPSLGLIVQAYLLEHEQLSDETLYFIY
                     SGSNDYINVLFFEDNYNTEVMSTYIDNVLDGLSSAVLKLANAGARRFVIMGIPHVGDT
                     PKFVKTTDRDVLNAAVDLHNERLAKRIDAWKERYPTADFLFVNTEQYLKKAFEDPEKY
                     GFYNVKDACIDVKFPMFNAFIHSPFARNYVLQYTQVLQYRDKQFAPGENNYHMCSEPE
                     DYLFWDEIHPTTRAHNYLAYEVCMALEEHGYEVTCKMPESA"
BASE COUNT         5037 a         3532 c         2932 g         5404 t
ORIGIN      
        1 agactctatt ttattattga taatgtcagg ggctattctg tctattgctt ttgcaatttc
       61 ctcttccata tcctggtcaa tagtaatttt ctctaaaatc tcattttttt tatcgagaaa
      121 atccttttca atgaattgta gaaactgttc ggcatgagaa ccaattttag cttcaagggc
      181 actattgaca ttagcatgga tgtaattaaa aaatattttt tctttaaagg cttgtatttt
      241 ttctggccca acttccagct cctgtttctt tagcagatca ataaaataac ctatacgctc
      301 ttcacataat tttaccttta atcgttttgt atcatccaaa ccggtaatca attcttgaag
      361 tttactttca tcattaataa ttctttctgt cgcagcttta aaatcttccg gtgtttgcaa
      421 ctctctaaga aaataagcga gatcatattg gtagtttatt ttgataagat ggggttgaag
      481 ctccgcataa agttgcaaaa attgttcttt attctcttct ttttcgaaat cgccatccaa
      541 atataaatcc cttaatttat caaaagggct ggatggttta ttaattttat gacgttcttc
      601 gaataccctg tcaatggcat ggagtaatga gtactcacca tctgttttgg aaatgtaatc
      661 ctcagtaaat tcaggattaa ttttagcaag ataaggttga atttttttat attcatttga
      721 agcttcttct tttaattcct ccaatgtccg ctcgtacaga ttcatgtgca ccccatcttc
      781 tatatccttc gcaatatgcc ttaagctggc atatggttga tataaagtac tgaattgctc
      841 atgctctgta ccaaatatac tttttttcgt atcgattgca ctggtatctt ccttaacacg
      901 ttcaaggcgt tccatttctc ttgccaaacg tttttgtgtg cttgttgtat caattaataa
      961 ctccaaaccc gtttgatctt tttccaaagg taaattgaca atcagagaac tatagaactt
     1021 ttcaaatcgc tcactcacag tacgagaata cgtaccgggc tttaggccca aatgaaattc
     1081 cgcattatca gcagccgcca ttaaaatggt gccgaactct gcttgaattt cttttaaacg
     1141 ccccattgtt aattctcgtc cttctttacc aatatcagaa agctgcatct gcagtttagc
     1201 ggcagtactc aaaatctttt taactgaacc tggaccaaag gataatccat ttaatccttc
     1261 aacaaatgct ttcacattcg catctatttc ctctttggaa ggttctggag tgtcaggttc
     1321 cgcttttaac ttattgagct tgactggaat ttgatagagg ctttctgcaa ttaattgtaa
     1381 ataagaagta tctttcagtg acgtactttg ttgaagagca ttttcctcat tcataattct
     1441 ttgcaaagca ccactgaaat cttttgctgt ctttaaacct tctaaaaatt gcgtttgatc
     1501 ataagtacta tcgattttat ggaggtacgg ttgaatttcc ttgtatagct gtcgaaattt
     1561 ttccttgttt tcttcttttt caaagtctcc ttccaaatgc aaatccttaa gtttagcaaa
     1621 actactttca ggattattaa tgcgttggcg cattgacatc acttcatcaa tagctttcag
     1681 tagtgttgat gactctttat gttttgctat gaaattttta tcaaattttg gatcaacaga
     1741 ctcgagaaga ggttggattt tgtcataata actaaccgct tcctctttta attcgtcaaa
     1801 agaaggttta tatggattat tttgatctcg aaactcatca gtaatacgct ttaacaagag
     1861 atagggctga taaacgctat taaattcttc atgttctgtg ccaaaaacta cctcatgagg
     1921 gttagttgca ccagtaataa caggagtaac agatttttct ggtgtcttac cctttggtgc
     1981 cggttcttgt tttaaaggca gattactaac aagtccatta caaaaattac tgacaatagt
     2041 gcttatatta ttggagtaag agccaaactt ttgacctgaa tgatactcgg tatattctgc
     2101 agccgccctg aattgcgcat tcaattcgga atgagtgtcc ttaagtttac cgagtgttac
     2161 ttcaggactt tctttatcca gattagaaag ctgagtttcg agtttggtca aggcagccag
     2221 tatttttttc gcagaaccag ggccaaaaga caagccacta atccccttgg caaattcgcc
     2281 aatttgagtt tttatttttt ccctggccga atctgaaatt tccaggtcac catcaaaatt
     2341 atcgatggct tcctgaattt tacccaattg ttcactaatt aattgctgat ctgttttagg
     2401 cttaggccct tttgacaaac cagattctac aaccgcttgt tgtttcttcc atgcagtaat
     2461 aatattttgc ttgggaggtt cctgtttttc tgtggttttt aaataatcac ctatcagtgg
     2521 cataccctct aatggtttta gtaaatccaa gccttcctgg acgattgcct ttaatccggg
     2581 attatttcct gcctccataa catagtactt actgaaacag atattcatga ccagagcatt
     2641 aaacgtggtc atgacaaatc gcgtacggtg ataaatgtta ttggggtcac catagtcatt
     2701 taacgactcc agattttcca agccattctt catgtaatgt atggagttta ttaattgctt
     2761 gtacatggcg acttcaggac tgtctttatg aaattccaga tacggtattt tatcaaaatc
     2821 aagcgtttct ccatttgatg agagctgttt ccatatttca ggagaaagat tgtccttaag
     2881 ataagtttga aatttcttat tgaaaaaatc atcaaccgat ttggataact tcaatttact
     2941 catctgccca aagagtgttt gtttctcaag ttctgccccc cttgctacca acggagcttt
     3001 tgcatattgt tcttcgcgag cttcttcctc cttatccagt aaagtagttt tatcttgatt
     3061 taaatcacta attttctcat ttaaataatc atcaataccc gatttaagag ataccagatc
     3121 ggtgaggcga agaggagggt ccgttggttt tgaactggtt aggctgctta caagtcgagt
     3181 gttaatgtca tcatgcttca acgccaataa ttgtggttga aatgttttat aagccttgcg
     3241 gagaaattct ttatccgatt catccagaga atcaaacgat ggattttcat ggggatattt
     3301 tttctgaata taattaaaaa actccgctac ccctttctgt gctctttcaa gttctaaaat
     3361 ttggttagat gctgcaataa ccttcttatg gtaatactca accggtttat cgtcctccaa
     3421 agtaaatttt agtggttcaa acggttgaac ccgagtagtt agattagttg ttttattatt
     3481 cattgtataa gctgtctctt ctgagtgact catagttttt tcaatgagct ttgctttaaa
     3541 ctcagattga gcaaggcttt gctgaatact ggatagcacg gagtccttac aggataatac
     3601 ctgcttaaaa tgatcagagc tccataattg cttataatcg gagctgtata gtctggaaac
     3661 aatattgctg ccggtaggct gggtaagagc atctacaacc agtttgtcaa tatctgggta
     3721 taatgccgca aaatgtggct gaaactgttt gtattccttg attaaagcgt ccttttcact
     3781 ctgactaatg tttgccaaac tccacttgga ccatgctttg tgaatgctat tgtaggagcc
     3841 gattttatca aagaaacgat tggccgcggc aagtacattt ttgtcttcag agccaagtct
     3901 ggcttcatta agtctgcttt cctgattaga tcttctcttc tgaataaata catcgtccat
     3961 caaaacacct aaatcaggtg cagacgttaa ttttggcccc acatccagtt ttttgctatc
     4021 tccagcaaga aaacgaacga tttttccccc tattgaatca gaataatctg aaacagtatc
     4081 aagaacccca gctgccttgg caatattatc aacttgctct gccagctgcg tatagtattt
     4141 attcatttgt tcaagagcgg gatcggtcag taaacctggt tttaaaccca tgctttcttc
     4201 aaccatttcc agctctgaaa tcaattgggg taaaatatta tgtttaatgt cttcaagctt
     4261 tgcgacagca tccaaataag cttgttttgt cagaggagca ccggcattaa ccagatcagt
     4321 actatgggca atcaatcttt tgactattga aagatagctt gggattgctg atagcccact
     4381 cttggaactg agttgttcaa aataatattt tgtttcattt gcctttttta tcattgcgga
     4441 ttgatagtct tcagcactgg taattgattt ggtggcaatc cctgatgccc cggtagcgat
     4501 taatttttgt aactcctcga agtaatgagg caattcaaaa ataaggttac tgagtttacc
     4561 cagactttct gactcggttg gcttttcagt tggaagcata ttgactacac cagcaagcac
     4621 tgctccagca ctctcctcag gatgctccgg ggcaaaattt cctaatgcct tactggctaa
     4681 agccatttgt ggtaataatt tttggatatg tggacctact atatcttgta tgtccgaatt
     4741 actgtgattg attagttgaa gcgccgcata aatttcatgc accgctttat aactgaccat
     4801 gactgcatct ttagcgatca tggctgtgta cctatcacga ctgatatcaa tattttcaat
     4861 agctcgaaag gatttttcag cattttccag agcattaagc aattttttta gaacagcgac
     4921 ttgggtagga tcagtcgtgt taactttaaa agggacattg gggttatctc gttcaaaata
     4981 agcagccaat tcctcagaaa aatgggtttt atcggtaatc gattttaaca aaccatcttt
     5041 tattgtttta caagtatcag ctatttttaa atcttctttt aaatagatga tgtaggtttc
     5101 aatggttctt ttcatgccac atcaaccagc aaatgaacaa tctatattat taatatagtt
     5161 gattcatgcc agtatagtgg ttttttctct caaaatttac ttcactttga atcaatattg
     5221 cgcaaaaact gggcgttagt acgtcatatc agatgatgtc ttttcttttt tctcctcagt
     5281 tccccataac catgaggtga gaaaattcaa gctaaaaaag ccaaaaccat tacttactga
     5341 aggttgaatt tgattttttt gcacttcgcg tttgcggtaa aaattataaa cttttatctg
     5401 ttgatagagt tcatcaaaca ccactacatc atcaatggat aattttttgc ctgtttcatc
     5461 tttattaccc gttgcgataa gaaagttact taatgcttct tgatcatata ctttcccttg
     5521 ggcagtaatg acaggtgttt tgataagcgt tttacttatt gggcaaagaa atatatccgg
     5581 aatttcagtg tattcttttt ggcgtgcagc aacccgagca ttttgcacta atctgttttt
     5641 taatttttca tagttcgttt gtgtctcaac aaatctattg actataatca ataactcggg
     5701 gaatggttgc aaatctttag ccgttaaggg tttacggctt tgtgggtctt cttttttcgt
     5761 ttctaaccat tttaaaatag aggatttatt ataaacaaaa ccttcaggtg tgatcacggg
     5821 atcttttaga aatccgctgt caatagggca agtcagcatt gttggtgttg tttctggatg
     5881 agataaattg gcttctaatg ctgcttctct taagtattta cttttatgat caatatcaaa
     5941 aggatttcgc gtcgccattt ctattctata ccccaactaa atgatgcaat attttaccat
     6001 tagatttatt tttatgcaat cgatttgttt tataggcgaa tcactccttc attatttgtg
     6061 ctttcgctaa taaagagcac cgtatctcaa gctgccagta aaaaagacta catgcttata
     6121 gtagttcgag gagggtcccc caatcaattg atcatataat tgtttattta aaaatcacaa
     6181 agtcaaaatt aattactata ataaaactat aagaaattaa attctatttg agacttgctg
     6241 taattatgac aaaaagccgc aaattaaaaa ggaatttact ggaaatatca aagactgaga
     6301 caggacaata ttctgttagt gcgcctgagc acaaaggttt ggtgcttagt ggcggcggag
     6361 cgaaaggcat atcctatttg ggcatgatac aagcgttaca agagcgcgga aaaataaaaa
     6421 acctgaccca tgttagcgga gcatctgccg gagcaatgac ggcgagtata ttagccgttg
     6481 gcatggatat taaagacatc aaaaaactga ttgagggatt ggacatcacc aagctattag
     6541 ataatagcgg agtaggtttt cgtgcaagag gagatcgttt tcgtaacatt ctggatgtta
     6601 tttacatgat gcaaatgaaa aaacatttag aaagcgtgca gcaacccatt cctccagaac
     6661 aacagatgaa ttatggcata cttaaacaaa aaatagctct ctatgaagac aagttatcaa
     6721 gagcagggat tgtgataaat aatgtagacg atattatcaa cttgacaaaa tcagtaaaag
     6781 atctggaaaa acttgataaa gcacttaata gtattcctac tgagttgaaa ggcgccaaag
     6841 gagaacaact cgaaaatcct cgtatcactc ttggtgactt gggccgctta agagagttgc
     6901 tgcctgagga aaataaacat ctaattaaaa acttgtctgt agtagtaact aatcaaacaa
     6961 agcatgagct ggaacgctac agcgaagatt caacacctca acaatctata gcacaagttg
     7021 tccagtggtc tggagcccat ccggtacttt tcgtacctgg cagaaacgcc aagggtgaat
     7081 acatcgctga tggagggatt ctggataata tgccggaaat tgaagggctt gacagagaag
     7141 aggtgctttg tgtcaaagca gaggcaggca ctgcatttga ggcccgtgtt aataaagcca
     7201 agcaaagtgc tatggaagcg ataagctggt ttaaggcacg catggattct ttggttgagg
     7261 ctacaatagg tggaaaatgg cttcatgcca cttcatccgt gttaaatcgt gaaaaagtct
     7321 attataacat tgataatatg atttacatta atacaggtga agtcacaacc acaaacacct
     7381 ctccaacacc tgaacaaaga gcgcgcgctg taaaaaatgg ctacgatcag acaatgcaat
     7441 tactggattc tcacaagcaa acatttgacc atccattaat ggcaatactt tatatcggac
     7501 atgataaact caaggatgca ttgattgatg agaaatcgga gaaagagatt tttgaggcat
     7561 cagcgcatgc acaagctatt ttgcatcttc aagaacaaat cgtcaaagaa atgaatgatg
     7621 gtgattacag tagcgtacaa aattatttag atcaaattga agacattctg acagtggatg
     7681 ccaaaatgga tgacatccag caagagaaag cctttgccct ctgcattaaa caagttaatt
     7741 ttttaagtga aggaaaactg gaaacttact tgaataaagt tgaggcagaa gctaaagcag
     7801 ccgctgagcc tagctgggcc acaaagatat tgaatcttct ttgggcacca attgaatggg
     7861 ttgtttccct gttcaaaggc cctgcccaag actttaaagt tgaagttcaa actgaaccgg
     7921 ttaaggtttc tacctctgaa aatcaggaaa cagtctctaa tgtaaaggat atcaatcctg
     7981 ctgtagagta tagaaaaatc atggctgaag gaaggcgcga gcataccgat ccatccccat
     8041 ccctgcaaga aaaagagaac gttgagcctt cagtcacatt tggcaatact tgatgttatt
     8101 tctaaaaata ggtttagtta cagaatcatt ccccgagata ttttcctatt tattcctgac
     8161 tcaagtgtgt tcttctttcc tgcatggttt ctatgctgtt gacaactgtt tggtaacata
     8221 tctctttcat tactggaata tctttcaatg gatatccttg atgaatagca taagctatta
     8281 aaaaactatc ctgccaaacc ttttcatttt tcctgtgttt tgccttaacc cataacgcta
     8341 tccataaaaa atgaaactgc catttatccc ggtgtaattc catttcctcg gaaaataccg
     8401 cgtccatagc atcagccaaa cgacaaactt taactccttc cacaaagctg ctgttatgat
     8461 tgacaatttt atcaatggtg ggattttcaa tataccatga ttctgtaaat tgcttatttt
     8521 taagccacat tttcgagcgt tttaaagatt ctgctatttt ttcttgagta aaaggaataa
     8581 tctgaacact taattgttca aatacgcaat ccaaatcaag cttatcaggt ttcaaacgaa
     8641 tgcccaggaa ttcgagtatt tctaaaaaat gcaaattagg tatatcactt ttttcaacag
     8701 taatggctaa gaaatgctcc actattgttt gaaaataaga gaaatcaact tcacgcaagg
     8761 tcacgctgtc ttcaaacgct ttatgataat aatctgtaac ttccgctgca gaaatctctg
     8821 gggttatcca ggcatcttta atgcccaaat catactttaa taataagccg cataacctgt
     8881 tttttttatt tttccttgcg tggataaaaa ctccttgcga accactgcca tccacctctg
     8941 ttgctttaat cgttattttt gcttgactag cctctgtttc aaatgttact cccttttttc
     9001 tttgcgtttt aatccaacga tcaaacattc catggtaatg ggtcggatac caatgtttga
     9061 ttatttgtaa tcttgacagt gatgctggcg tcaaagtaat tgtattcatt aattgatcga
     9121 tagtagacag cgcgatctct cgtacctcga tattaggatg gagtaaaacc agtagtgcaa
     9181 tatctttacc ttcttcaata ctatacaaat caacaatcaa atctgcaaaa aaatcaggcg
     9241 gcatggcata gctttgcgca aaaaaactct ctgctatgtc aaacagtgtt aaatcagata
     9301 attcatggat caaatctttt atggagtcca aatgattaaa ttgctcatcc acttctaccg
     9361 catcatcatc ctggcttgcc aagccataat atgcttcctt taaatcgtca gttagctcaa
     9421 catgcacttc ataaaatgca ttcagtacgg gtaaccaaaa gcttaagcta tgcttatgtg
     9481 tatcaatgac ctcagctaaa tgattcatta gttgagacaa taattttgtg gcatttttat
     9541 tatttgcctc tgcagctgct tgcaattgag caatacaaat gtctaaagcg aaaacgcatg
     9601 ctgagtatat tggaggccca tcatgcacta attcttcact caaattatta ataaggtgta
     9661 ttaattcaag actaatttcc ggatgttgaa aaaaaaaggc atacaattga gggtcaggct
     9721 ctgcatcttg ctcaatcaat acagccatat cagcaattac tcgctgcaac tcttgagttt
     9781 tattagtcat tgtgtttttg gtacctgcct cggctttcca ttttcatcaa tcgcaacaaa
     9841 aacaaatatc ccttcagtaa cctggtattt ttccattttg atcgatgagt aagcccatac
     9901 ctcaacccga atagtcatag aggtattacc cactttcact aattcaacgt ggcaactcac
     9961 tacatcgcct acatgcacag gttttaaaaa agtcattgaa ttaattgcaa cggtagcgac
    10021 acgcccatgg gaaattctct ttgccaaaac acctgctgcc aaatccattt gggagactat
    10081 ccatccgcca aatatatcgc cattggcatt agtatttaaa ggcatggcca aagtttggat
    10141 agtcaactct cctttaggtg tttgcatccc tattctccac cacgttttag tttggctaaa
    10201 gcatcggcca tcgctgtatt aaatacggtt tttttgactg gctccgattt ttttatctta
    10261 tctggttttt tcttatgttc ttgcttcttc ttaaattcag gctgcttttg ttccgcttgt
    10321 tttcttgccg gctgtggttt attagctttc tgcaatacag caggagcctg ctcatcattc
    10381 agcttcatac ttaaaccaat ccttcggcgt tctttatcca cttcaaccac tttaactttt
    10441 acaatatcac cggctttaac aacagaacga ggatctgtaa taaagcgatt ggtcattgct
    10501 gaaatatgta ccaacccatc ctggtgaacg ccaatatcaa caaaggcacc aaaattagtc
    10561 acattactta caacaccttc cagaaccatt ccttgttcca aatctttaat gtcctcaaca
    10621 ccttccttaa attgagcggt tctgaattct ggtcttggat ctcgccccgg tttctccaat
    10681 tcacgtaaaa catctcttat tgttggcaaa ccaaaattct catccacata ttgttcagcg
    10741 ttaatactct gcaataattc tctattgccg attatttcct taatactaac ttttttatct
    10801 gaaataattt tttccactaa aggataggct tcaggatgaa cacaagaggc atctaaagca
    10861 ttatctccat tcataatgcg caaaaagccg gctgcttgct gaaaagcctt ttctcccatg
    10921 cgattgatat tttttaattg atttctgttt gtgaaagcac catgttcatc gcgaaattgc
    10981 acaatatttt tagctaaagt atcattcaat ccagaaatat gggatagcaa agctgcagag
    11041 gccgtattaa tgtctacgcc aacagcattc acacaatcct cgacaacacc atccaggcaa
    11101 cgcgccaact ttgcctgatt cacatcatgt tgatactggc caacacctat tgatttggct
    11161 tcaattttta ccagttctgc caagggatct tgcaatcttc gtgcaataga tacagcgcct
    11221 cttagggaaa catccaaatc aggaaattca ttggctgcaa tttcagatgc ggaataaaca
    11281 gatgccccgg cttcactgac aattattttt gttaatttca aatcagggta cattttaatt
    11341 aaatcagcta ccaatcgctc tgtttcccga gaaccggtgc catttcctat gctaattaaa
    11401 ttaacctgat atttcgcagc aagctttgcc aagtccgcaa tggattgatg ccattcattt
    11461 tgaggggcaa atggaaaaat aacagagtaa tccaacagct tacctgtagc atccacgaca
    11521 actactttaa cacccgttct aattcccggg tcaagaccta tcgtcacttg aggacctgcg
    11581 ggagcagcca gcaacagatc acgtaaattt ttggaaaaca ctttaatggc ttcctcatct
    11641 gctatttcgc gcagacgagt taataactca agctccagct tggtaaataa cttaattttc
    11701 caagtcatcc tgaccgtatc tataagccag ccatccgctt ttctttgctt gtcagtgata
    11761 ttaaagtaag cagcaacctt attctctcca taactggcat cctgatcagg caaaaccaga
    11821 cttacttgca gaattccttc acgccgcccc cggaacaagg ccagtgcccg atgagatgga
    11881 atttttttaa tcgcttccga ataatcaaag taatcagaaa atttattcgc ttgatccttt
    11941 ttagtactgt ttcctactga tttgactatt ccgtgttgcc acaaatactc tcgtaattcg
    12001 ttaattaact cggcgtcttc agcaaaatgc tccattaaaa tatgtcgcgc accatccagg
    12061 gcggcctgag tgttttcaat ccctgcttcg ctatttataa attgggaagc ctgttcttcg
    12121 ggatccaaag caggatcttt ccataaagca tgagctaatg gctctaatcc tgcttcaata
    12181 gcaatttgcg cttttgtacg acgtttaggc ctatagggta aatacaagtc ttccaggcgt
    12241 gttttagttt cagcagctaa tatgctttgt tccaattccg gtgttaattt ttcttgttca
    12301 cgaatggatt gcaaaatgac tgttcgtctc tcatccaatt cacgcaaata aattagtctt
    12361 tctgcaagaa accgcaattg cacatcgtca agccctccag ttgcttcttt gcgataacgc
    12421 gcaataaaag gtactgttgc tccttcatct aacagccgaa tagcagtttc tacctgcgat
    12481 actttgacct taagttcatc tgcaataatt gcggctgacg ccaacatcac ttctgccatt
    12541 catatccccg taaaacatga taaaaatcgt ctcattatat actcatcaca tactataatg
    12601 cgatatagaa attaagctaa tatataaaca aattgtcctt atgcgtaaca ctgtactgca
    12661 ttttacccct tcctgataca ccagacataa tcattcactt cactaatacc aagattaaat
    12721 aaatgatcat taggttcctg aattcgtttt tgcgatttca acaactctat tttggcacat
    12781 tgctgatacg ataaagcgat ttcttctggg gaaacactat acggaggacc ctgcactttc
    12841 tcttgattat attgtaaagt ctttaacaga atacttcccc caggcttaag ccacttgagg
    12901 caaatatcta catacgctgg tcgcaatttt tttggcaaag caaccaaagc cgctctgtca
    12961 tagatagcat caactggttc tattaaagcc gagtttaaag caaaaatatc ggtaacccat
    13021 aaattcaaat tatctgtaaa atattgtttt gcttgaccta tcgtattctc ccgaacagtg
    13081 atttggtgct cctgaacaaa ctgaagaata gccttttcaa ccagttcgat accaaccaca
    13141 tgataccctt ggcgaactaa ccaaagcata tcgacacttt ttccacataa aggaaccaaa
    13201 acccttccct tggcaggtat atttaaggaa gagacatagg ctatcaaatc cggatttact
    13261 tcttctttat gaaaagaaat acgtccctcg caccataact cattccaaaa atattgacct
    13321 ttgttcactc cacagtcacc gattttgcaa ggtttctagg ttgatccaca tcagtccctt
    13381 tagctacagc aacatgatag gccagcaatt gcaaaggaat agtgtaaaca ataggcgcaa
    13441 gccaagcacc acaggatgga actttgatta aacgagcacc attcgctttc caattttgtg
    13501 aatcatcaac aaatacaaat aactgcccac ctctggcgct cacttcatgt aaattggatt
    13561 ttaatttatc taaaagttca tcattagggg ctactgcaat cacaggcata tccttatcaa
    13621 ccaaagccaa tggaccatgc tttaattcac cggcaggata agcttccgca tgtatataag
    13681 atatttcctt aagttttaat gcgccttcca gggctacggg atattgaacc cctcgaccta
    13741 aaaataaggc atgaacttta tttacaaaca aagaagccaa tgattcgact tcttcattca
    13801 tctgcaaaac acgctcacaa caggcaggca gctcttgtaa ttgtcttaaa acttcctgcg
    13861 cgcgattgtc cttacaaagt gctgctgcca acatcaggaa ggctgccaac tgtgtagtaa
    13921 acgctttggt agatgctaca ccaatctcaa tacctgcccg agttaaaaaa acacaatccg
    13981 cctctctcac taaagtactg gttgcaacat tgcatatagc taaactagcc aaataattcg
    14041 attgctttgc cttaaatagg gcagcaagag tatcagctgt ttctcctgac tgagatacag
    14101 tgataaataa ggtattgtcg gggacaacaa catctcggta tcgatattca cttgcaattt
    14161 caacctgtgt aggtaagccg gcaagtgatt caagccaata tttggcaatc attccagcat
    14221 gataactggt tccacatgcc acaatgtgaa tattttttac cattgggaaa atatgggatg
    14281 ctttctcacc aaaacttgct cttagcacat caatgctatt aattcgactt tctaacgtgt
    14341 ctgtgattac cttcgattgt tcaaatattt ctttcagcat aaaatgcctg tatggccctt
    14401 tattcactat ctcagaatca ctgttcaacg gatgaaccgc tctttgaaca agtattcgat
    14461 tggaatcata aaccttaata tcctgtgttg tgacacaagc actgtcaccc tcttccatgt
    14521 aaataactga ttgtgcgaaa gatcttagtg ccaaagcatc cgatgcaatg aaattctcac
    14581 caattccaaa tcccaaaacc aaaggactcc ctttacgaat tgcaaccaat tcttctggtc
    14641 ttttctgatg aatcactcct aatgcaaaag ccccttgcat ttctgcagca gctttttgta
    14701 cagcaagtaa taaattctca tgctgctggt aatgataatg aattaaatgc gcagcaacct
    14761 ctgtatcagt ttcagatgtg aattgatagc cataagtaat aagttgttga cgcaaatgtt
    14821 catgattttc aataatgcca ttgtgaacta atgcgatttc tccatgagat aaatgaggat
    14881 gagcattttg ttcggagggt ttaccatgcg tcgcccaacg agtatgagct atacctgtat
    14941 ttccagcgat agcggtctct tgcatagcat ccgccaaatt ctgcactttc ccctggattc
    15001 ttactctctt taaacgatct tgactatcta tgacagcaat acctgcagaa tcgtaccctc
    15061 tatattccaa tcggcgcaag ccttccaata aaatcttgct aatatcccgt tctgaaacag
    15121 ctcccataat cccgcacata acctgctcct cttttcagca actgtttttg tagaaataag
    15181 cttttttcat tcttggttca tctacaacat atagataaag agtaaaacgc ataagaatgc
    15241 agctaatttc ctaaataacc ggtgccttaa attttataaa taatattccc tcatatgaat
    15301 gaagtatatc aataaaaaag gggaattatg acataaagtt ttacaaatca ctaaggcaaa
    15361 ttaatcttcg tcatcattta gagactcaag aattgcttaa aaactactct ggatttactt
    15421 aaatgaggat caattttgac aacttcatca tgaaatcacc tgaaccactt ttaatgtgat
    15481 tcaggtgatg atgcctaatt gtattaggcg gactccggca ttttgcaagt tacctcgtag
    15541 ccatgctcct ccaaagccat acaaacctca taagcaagat aattatgtgc tctcgtggtt
    15601 ggatgaattt catcccagaa taaatagtct tctggttcac tacacatgtg atagttattt
    15661 tcacctggag caaactgctt gtctcgatat tgaagaacct gcgtgtattg caatacgtag
    15721 ttacgagcaa atggagagtg aatgaaggca ttgaacatgg ggaacttcac atcgatacag
    15781 gcatctttca cattgtaaaa accatatttc tccggatctt caaacgcttt tttcagatat
    15841 tgctctgtat tcacaaacaa gaagtcagct gttggatacc gctctttcca tgcgtcaatc
    15901 cgttttgcaa ggcgctcatt atgaaggtct accgctgcat ttaacacgtc acggtcggta
    15961 gttttcacaa acttgggagt atcccccaca tgtggtattc ccataataac aaaacgtctc
    16021 gctcccgcat tagccagttt cagaactgca gaactcaaac catccaacac attgtcaata
    16081 taggtgctca ttacttcagt attgtagttg tcttcaaaaa acagaacatt aatgtaatca
    16141 ttacttcctg aataaataaa atacagcgtt tcatcactta actgctcgtg ttccagcaga
    16201 taagcttgca ctatcagacc aagactcgga ggaattaatt ttcctactat caggttcttg
    16261 attgttccca atggatgccg tatcaagttc caaactgtta attgataatc ataagtggca
    16321 gaccagctac ctccaaatgc cttatttagg tagacttctt catcactttt ttgtatcgat
    16381 aacatttctg ccaaatactc aatccagaca cgaccatcag aaaagcgatt tttccaataa
    16441 ggtttgccag gcagcaccgg gacctgtttt actttaccca ccaaattggc aagataggga
    16501 gccaaatcat gatcaaaaaa atctgtaact gctgatattc ctgcatcaag taccatctgt
    16561 ggaacataat aatcgttagc aaactctatc attttgttga taacgaaaat tttgaaagga
    16621 gccactaaaa acgcaggatc ctcttcctga cgcaggcttt taagtaaatg ggtagtatta
    16681 ccattatcag acaagctatc tccaaatacc actattgatt tcactggaat ggacgtaaaa
    16741 ccaaccaaag ggagagacaa caatacaatg aagagccagc gacaaatcct gttgttttgg
    16801 atcataaaaa tctccttata tcaaagctag cgaaacaaaa gatagcagct taacccttag
    16861 agtatgatca gaatttaatt attgtcaacg attctttata cttaa
//
LOCUS       DAXLDT010000063        16544 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXLDT010000063 DAXLDT010000000
VERSION     DAXLDT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16544
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.063"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            178..1140
                     /locus_tag="ACF2SP_001642"
     CDS             178..1140
                     /locus_tag="ACF2SP_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFD2359430.1"
                     /translation="MNFCTLGLAVIETEAQAVFELTQRIDEHFEKACELLLSCKGRIV
                     VTGMGKSGHIANKLASTFSSTGSPAFFMHPGEASHGDLGMITRQDIVVAISNSGNTHE
                     IVTLLPLLKRLEVPLITLTGNKQSTLAKSADVNLDVSIKQEACPLGLAPTTSTTVSLV
                     MGDALAISLLQARGFSAEDFALSHPGGALGKKLLLKIDDLCHTGEQLPLANENATVSD
                     ALIEVTNKKLGMTCVVDNHGYLVGVYTDGDIRRTLTRQFNINTTLIKDVMTKNCRTIS
                     KGMLAAEALAIMQKYSITSLVVVENDNRPYAVLHLHDLLKAGVF"
     gene            1154..1681
                     /gene="kdsC"
                     /locus_tag="ACF2SP_001643"
     CDS             1154..1681
                     /gene="kdsC"
                     /locus_tag="ACF2SP_001643"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFD2359431.1"
                     /translation="MNELLEKAKKIKCLICDVDGVLSDGLLHIDNHGNELKSFHVQDG
                     MGLKLLMAAGIQVAIITTAQNAVVDHRMEQLGITHYYKGQVDKRSAYQHLKKTLGLND
                     DEFAYIGDDLPDLPLIQQVGLGVAVSNAVPQVLEFADWRTERTGGRGAVRELCDLILN
                     AQNKAELAITGYLKQ"
     gene            1678..2247
                     /gene="lptC"
                     /locus_tag="ACF2SP_001644"
     CDS             1678..2247
                     /gene="lptC"
                     /locus_tag="ACF2SP_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFD2359432.1"
                     /translation="MNITKQAIWLFLTLLILAGSGWYYAHFNQAIINKKSLSNEVDTT
                     ISQLTVKQFNSEGVLINLLTTPQVEYIPNNNLHLLKNPHIIIKQDNQPAWEINSLRAK
                     SFDGGKQITFIEQVVVHQNPGESTRESTLKTEEITYYPKEKKATTSLFVTFEQPGNII
                     QSTGMNAYLDEKRVELLHKARGSYDPAKG"
     gene            2228..2737
                     /gene="lptA"
                     /locus_tag="ACF2SP_001645"
     CDS             2228..2737
                     /gene="lptA"
                     /locus_tag="ACF2SP_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFD2359433.1"
                     /translation="MTQLKVKYIILLLLMLCSISGNALPFDKEKMVQVVSDSADLNQK
                     NHQGVYIGNVEFVQGSTNLRAEKAITQGDAKNQLTLAVAEGSKDKQAHYWTTTAPDKP
                     PFHAYADTIKYYPLKHLIELIGNARIEQGPNSLSAAKISYDTQEQHVLSHSDEKTRTT
                     IIIYPEKKS"
     gene            2734..3459
                     /gene="lptB"
                     /locus_tag="ACF2SP_001646"
     CDS             2734..3459
                     /gene="lptB"
                     /locus_tag="ACF2SP_001646"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFD2359434.1"
                     /translation="MSLLEAKQLKKSFKGRTVVNGVSIHIKQGECVGLLGPNGAGKTT
                     CFYMIVGLQSCDEGHIILNGTDITQDAMHLRARHGISYLPQEASVFRKLTVAENIMAI
                     LELRKDLDDQKREEKLDLLLQEFHISHIENSLGMALSGGERRRVEIARALAIEPSFIL
                     LDEPFAGVDPISVIDIKKIILHLCDKGIGVLITDHNVRETLDICERAYIVSQGKILCE
                     GTPKAILANQQVRQVYLGEEFAL"
     gene            3493..4071
                     /locus_tag="ACF2SP_001647"
     CDS             3493..4071
                     /locus_tag="ACF2SP_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFD2359435.1"
                     /translation="MLLIIDNYDSFTYNLVQYFQSLNQKVEVFKNDEIQIDTIRQSAP
                     QYLVISPGPNSPDESGISIEVIKEFHKHIPILGICLGHQCIAQAFGGKIIPAPEIMHG
                     KTSEITHHYQGLFTGIPNHFQATRYHSLAVDIMSLPNCFSIDAWADDTIMAISHRQFP
                     LFGLQFHPEAILSQFGLQILENFLRYETQTSI"
     gene            4049..5083
                     /gene="trpD"
                     /locus_tag="ACF2SP_001648"
     CDS             4049..5083
                     /gene="trpD"
                     /locus_tag="ACF2SP_001648"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFD2359436.1"
                     /translation="MKPKLLFEQLLSRQDLSSDQMQEVIHACMTGEFSDVQIATFLAL
                     MRMKGETVNELTAAAQVMRQLAHKIDLGNPLIDIVGTGGDGRNTFNVSTACSFVVAAA
                     GIKVAKHGNRSVSSRSGSADLLEQAGFILNLSDSQVQNCINQCQLAFLFAPHYHPAMQ
                     HARAARQQLGIRTLFNLLGPLINPAQVKRQVVGVFSTNWLKTIATVLANLGSERSLVI
                     SSQDGLDEISIAAKSEVVEYRDGNFKQWFISPEDYGLKHSSLDAIIVDSPEQSLHLIQ
                     SVLSGDSGPARDIVLLNSAAAIYCAKDGISFDAAIEEARIAIDSGKANLCFNKLRLLT
                     QTLNKESNHE"
     gene            5076..5852
                     /gene="trpC"
                     /locus_tag="ACF2SP_001649"
     CDS             5076..5852
                     /gene="trpC"
                     /locus_tag="ACF2SP_001649"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFD2359437.1"
                     /translation="MNSILERIAKHKLEEVAVAKKNKPLHVLSKQQPGEMRDFITALK
                     SNTSPAVIAEIKKASPSKGLIRKDFNVAEIAKIYTQNGARCLSVLTDIEFFQGHPDYL
                     ALAKSKTTLPVLRKDFIIDSYQIYESLVLGADCILLIVALLDDVQLMDFCQLAQELKM
                     SVLVESHTQDELERALRLPTPLIGINNRSLHNFKTDIQLSIQLKQFVPKDKIIITESG
                     INTREDIKLMQSHGINAFLIGESLMRADNIGKALQKLMTD"
     gene            complement(5858..6025)
                     /locus_tag="ACF2SP_001650"
     CDS             complement(5858..6025)
                     /locus_tag="ACF2SP_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359438.1"
                     /translation="MNTTEIPEQPIEYPEEPSEYPEQPLEYPDNPEPLEPGYPELPEP
                     EPSQTPEPGFE"
     gene            6328..7176
                     /locus_tag="ACF2SP_001651"
     CDS             6328..7176
                     /locus_tag="ACF2SP_001651"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2359439.1"
                     /translation="MNVVIITGAASGIGHALSQVCLSNGKTIVMVDKNQDKLHTEAQS
                     FMTKFSPEHVICFACDVTQPSEVERLAEYSCKQLGQVDWIFNNAGIIGNLLPAWELQE
                     NDINQVMQVNLHGMLNIIRSFMPYLFKQNFRSHIINMASLYALCTGSQMAAYSMSKHA
                     VLALSESLYFDLNRLKKPVDVSVVFPSFTDTSLLASSGKLNHSPIHNQLNNLLAHSRP
                     AIEVAEHIVREVEQKRFYILPDKEVKGYCEDRTKAILLQENPHRNSVEQLMCSLLKRQ
                     SSEANL"
     gene            complement(7269..8609)
                     /locus_tag="ACF2SP_001652"
     CDS             complement(7269..8609)
                     /locus_tag="ACF2SP_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HFD2359440.1"
                     /translation="MEIKRVLKDRLSARVCVIGAGPSGIAAIKNLQEHGITNVTVFEK
                     NNQIGGNWVYDEQNEHSSVYETTHIISSKRWSEFEDFPMPVDYPDYPSHSQLLKYFQS
                     YVEHFHLDRYIRFNTVVQKVHRLDDNTWHVIYEDAQGIHEACYDYLLVANGHHWDPFM
                     PVYPGVFDGEILHSHQYKKASIFKGKRVLVVGGGNSACDVAVEISRVAPGTCISMRRG
                     YHIFPKFIFGKPTDVAVAKIQWMPSWLRQKFISLVIRGLQGRYAKYKLMKPDCGPLEI
                     HPTINSELLYFIRHGKIHPRSGISRFEGNTVHFTDRTQGEFDTIIFATGYQISFPFFD
                     KSCIDFSNSTTIPLYRKMMHPEFENLYFIGLCQPQGCIWPLADYQSKIVARIIAGTLK
                     RPDQLHRKIAKEINKPHHRFKSHMRHALEVDYHIFRKELLEMLGQKHKRLKFYP"
     gene            complement(8587..9312)
                     /locus_tag="ACF2SP_001653"
     CDS             complement(8587..9312)
                     /locus_tag="ACF2SP_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HFD2359441.1"
                     /translation="MNYFGKLQIRFSKSFLLLLIVSSLTACVDLTGARNTYIKKEKQP
                     AISDVPNKKSKAQNMSSKKGEVHTMLGGLGIFSKGMYTLRDKVSEKYHITAHSTMWYN
                     AGAVSRYIIDHYYQEKDHKPIILVGHSLGANEQIKVARNLNAAGVPVALLVTVDAVSQ
                     TIVPPNVKQALNVYKPGFVPMFSGLKLRAVDPQLTKIDNINVDSIKGIKVNHFTIDKD
                     EILQAMIMDKVDKVLVDGNKKGA"
     gene            complement(9448..11367)
                     /locus_tag="ACF2SP_001654"
     CDS             complement(9448..11367)
                     /locus_tag="ACF2SP_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HFD2359442.1"
                     /translation="MTLTKKMAVGVLIMLLFVFIGTYFITMNNARNFFIQQLESNAQD
                     TATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVQDIKGKVIILKKQLPQESD
                     IPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFA
                     LVALVLSYGFLKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTK
                     VKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVID
                     GLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFE
                     KECQEFEQILSQGINDIKICKIHMGAASYFLHQPVSNLLSTVDQAVKKARETGVFYCQ
                     KEHDTYKYPQLISGDEIRNSLQQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGA
                     GYFIPVAEKLGLAYPIDQYVLNELTVMDIATHTHFALNISEDTLANKVNSTGYLRQLE
                     DTPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDL
                     NIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKTMDIAVVATQVESEAQWQALQIVHI
                     PWGQGRFLSSVELIK"
     gene            complement(11383..12117)
                     /locus_tag="ACF2SP_001655"
     CDS             complement(11383..12117)
                     /locus_tag="ACF2SP_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HFD2359443.1"
                     /translation="MLKESWLLSKSRPRRLSVIAKRFQKVFLESLRYRLVLSIWTGLI
                     LCYVTSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWGNLIDSLKKKPVKIQLEKVN
                     SFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDCEDFAIIKYFTLVAVGVPSDQLRI
                     TYAASLTLNQAHMVLSFYPTPESEPLILDSLESKILKASARPDLKPVYSFNAEGLWLA
                     KTRGESSLMGDSKSLGKWDALMKRME"
     gene            complement(12068..13792)
                     /locus_tag="ACF2SP_001656"
     CDS             complement(12068..13792)
                     /locus_tag="ACF2SP_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFD2359444.1"
                     /translation="MRKALLVIAMLTSSYVAADTLYEAVQYGMIANPDILLNTAKGLS
                     AKQAIDKAKGGLYPSIDVTGGFGRQRSVNPTSAAIDDTPSTTLNIVESAVELRQRLFA
                     GGGIINEVKRNQYLTEAQKWKTQGIAEDLALEITKNYFAVLLHERLYAYSIENLKAHK
                     AVFKMIKERATAGITRTAEVDQANARLALAEANKISALADLQEVKINYAKTVGKWPEN
                     LQVPHVPARKSLPNNLARIIEKGLDNHPTVKSSYADIKEAKAQYDVARAAYYPEVNLV
                     LNSSKNKNLGGLIGPNDSDTVAVRMNYNAFRGGADAARIRETAYQVQEAYETKNRTLL
                     ELKETIRLAWNAYVASALRIQPLKQHVTASRKTRTAYQDEFKVGKRTLLDLLDSQNEY
                     YESQIELARAENDEILSRYRILNGMGCLLKHLNLRLPVNVVNNDVFSSAQTNILLNRK
                     MDEIPYPNNTDNPMVLTHPVKNMEKTRLTKSMVDKNTTYPQQITPKLWYISAGSFRDK
                     NNAVALVKRLNGLGFTAFMSPYKDLLSVFVGPYEYRGHAGNGMERLKELAHVEGVLVT
                     FKEPPKKA"
     gene            13942..15273
                     /gene="xseA"
                     /locus_tag="ACF2SP_001657"
     CDS             13942..15273
                     /gene="xseA"
                     /locus_tag="ACF2SP_001657"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFD2359445.1"
                     /translation="MSSQLPILTVSQLNRQVKGFLENEIGLVHVEGEISNLSKPSSGH
                     YYFTLKDSTAQIRCAFFKNRHSNSLLRNFNDGQQIVATGKLSLYEARGEYQLIVEEIV
                     KAGMGILYQRFEELKIKLASEGLFNPERKKPLPRIPETIGIITSPTGAAIQDILSTLA
                     RRFPIARIIIYPSEVQGQTAPQQLVKALKLANAHKRCQVLILARGGGSIEDLWAFNDE
                     YLARQIAISEIPVVSGIGHETDFTIADFVADYRAETPTAAATAVTPNCIELFNILDTA
                     IYRLHDAIIRLVKGLQLKLNHLIDKIASPRQTISTYWQTLDYLERQLISAMTQFINLN
                     INKVNIFSTQLQANNPKTQIERTKTQLRQLIMQLNQEIRIQVNQLKNQLSTNLSTLHA
                     VSPLATLDRGYAIVSKNQRILFAAQQAQIGDTIDVRLAKGSLACEVTQIKD"
     gene            15282..16184
                     /locus_tag="ACF2SP_001658"
     CDS             15282..16184
                     /locus_tag="ACF2SP_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFD2359446.1"
                     /translation="MKNLLHSLACLLILISSAKLSYGLALPENHSVNGGLTIIPIDFN
                     QKPEAYFEGKRIPVLPSTQPRQWLLIVAIPLKNTNSVQYINVTKPIKASIPFHVSEKF
                     YSTQFLNIKDISKVDPQPEDLIRIEKETKKLTQIFANYSNTNPFEKQFTAPLRGPITS
                     LFGLKRVYNKQPRDPHSGLDIAADEGKPVYAVNQGVVADTGDYFFTGNTVILDHGMGV
                     FSVYAHLSKILVKTGETIKQGQELGLVGMTGRVTGPHLHWTMVVNQTLVEPLLFVPFR
                     KITVTPVIPDKKSNKSTQDATAKS"
     gene            16181..>16544
                     /locus_tag="ACF2SP_001659"
     CDS             16181..>16544
                     /locus_tag="ACF2SP_001659"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFD2359447.1"
                     /translation="MINELTTSLNVIVTQTQNNLKILGVILLIPWSIFFISHFISNKI
                     LLLGIIPRHILGLPGILLAPLLHANFNHLFFNSIPLLVLSNFILINGVNYYLIVTVMI
                     TLFSGTLIWCFAKRGIHIG"
BASE COUNT         5225 a         3326 c         3012 g         4981 t
ORIGIN      
        1 taatcataaa aattaaataa aagacgtttt ttattattta agttcaactt gagtgttatt
       61 ttaataaata ataatgttat aagccattag ttggtatcag ttactgaaat atagggtaca
      121 tcatttaaat acattatgtt agaatgaaaa ccaaacaaaa ttacgactca atgacctatg
      181 aacttttgca cacttggact agcagttatt gaaactgaag cacaagctgt atttgagcta
      241 actcaacgaa ttgatgaaca ttttgaaaaa gcttgtgaat tactcttatc atgcaaagga
      301 cgtatcgtcg tcacaggcat gggaaaatct ggccatatag ctaataaact ggcctctact
      361 ttttccagca ctggcagccc cgcttttttt atgcatcctg gagaagcaag tcatggagac
      421 ttgggtatga taactcgcca ggatatagtt gttgccattt ccaattcggg caatacccat
      481 gaaatagtta ctttgcttcc attattaaaa aggcttgaag tccccttaat tacattgacc
      541 ggcaataaac aatcaactct ggctaaatct gccgatgtca acctggatgt cagtattaaa
      601 caagaagctt gccctctggg tcttgctcca acaaccagta ctacggtatc gctggttatg
      661 ggggatgcat tggctatatc tcttttacaa gcacggggat ttagtgcaga ggacttcgct
      721 ctttcccatc ctggaggcgc acttggcaaa aaattgttgc taaaaataga cgatttatgt
      781 catacaggag agcaacttcc cttagcgaat gaaaacgcaa cagtgagtga cgctttaatt
      841 gaagtcacga ataaaaagct gggaatgact tgtgttgtag acaaccatgg ctatttagtg
      901 ggtgtatata ctgatggaga tatccggcga acattgacac gtcaatttaa tatcaacact
      961 actctaatca aggatgtcat gactaaaaac tgcagaacta tttctaaagg catgttggct
     1021 gctgaagctt tagcaatcat gcaaaaatat agcatcacat ccttggtagt agttgaaaat
     1081 gataatagac cttatgcggt actccattta catgacttgc taaaagcagg tgttttttaa
     1141 tacagagata gaaatgaacg aactattgga aaaagcgaaa aaaattaaat gtcttatttg
     1201 tgatgtggac ggggtgctca gtgatggctt acttcacatt gataatcacg gcaatgaact
     1261 gaaatcattc catgtacaag atggaatggg attaaaatta ttaatggccg ccgggataca
     1321 agttgcaatc attaccacag cacaaaatgc tgtagttgac catcgaatgg aacaattagg
     1381 gataactcat tactataaag gtcaagttga taaacgtagc gcttaccaac atctaaaaaa
     1441 aacattggga ctcaatgatg atgagtttgc ctatattggc gatgatttgc cagatttacc
     1501 cttgatacaa caagtgggat taggagttgc agtcagtaat gctgtgccac aagttctgga
     1561 atttgctgat tggagaacag agcggactgg tggtagaggc gcagttcgtg aattatgtga
     1621 cttaattctt aacgcacaaa ataaagcaga attggcaatc acaggctatt taaaacaatg
     1681 aatattacca aacaagcaat ttggctattc ttgaccttgc ttattttagc aggatctggt
     1741 tggtattatg ctcattttaa tcaggctatt atcaataaga aatcgttgtc aaatgaagtt
     1801 gacacaacga tttcgcagct taccgtaaag caatttaatt cagaaggtgt gttaattaac
     1861 ttattaacca ctccacaggt agaatatatt cctaacaaca atctccattt gcttaaaaac
     1921 ccccatatta ttattaagca agacaatcaa cccgcgtggg aaatcaattc attacgagca
     1981 aaatcgtttg atggtggtaa acaaattact tttatagagc aagtagtagt tcatcaaaat
     2041 ccgggagaaa gcaccaggga aagcacttta aaaactgaag agattactta ttatcccaag
     2101 gaaaaaaaag caacaacaag tctttttgta acctttgaac aaccaggcaa tatcatacag
     2161 tctactggaa tgaatgccta tttggatgaa aaaagagtag aattactcca taaagccaga
     2221 ggtagctatg acccagctaa aggttaaata cattatacta ctattgttga tgttgtgctc
     2281 catttcagga aatgctcttc cgtttgataa ggaaaaaatg gtgcaagtag tttctgattc
     2341 agccgattta aatcagaaaa atcatcaggg agtctatata ggaaatgttg agtttgttca
     2401 agggtcaacc aatttgcgtg cagaaaaagc cattacgcaa ggagatgcca aaaatcaact
     2461 cactttagcc gttgccgaag gctctaaaga caagcaagct cattattgga ctacaacagc
     2521 ccctgataaa cccccattcc atgcctatgc tgacactata aaatattatc cgctaaaaca
     2581 tttaatcgaa ttaattggga atgctcgcat agagcaagga cccaactctc tttcagcggc
     2641 aaaaattagt tatgatactc aggaacaaca tgttttatca catagtgacg aaaaaacaag
     2701 aacgacaatc attatttatc ctgaaaagaa atcatgagtt tactagaggc taaacaatta
     2761 aaaaaatcat ttaagggccg gacggttgtg aatggtgtta gcattcacat taagcaaggt
     2821 gaatgtgtcg gtttattggg tcctaacggg gcgggtaaaa ccacttgttt ttatatgatt
     2881 gtaggtctgc aatcctgtga tgaaggccat attattctta acggaacaga cataactcaa
     2941 gacgcaatgc acctaagagc aaggcatggt attagttacc ttccgcaaga agcctccgtt
     3001 tttagaaagc ttactgtcgc tgaaaatatc atggcgattc tggaactaag aaaagattta
     3061 gatgatcaaa aacgtgaaga aaagctggat cttctcttac aagagtttca tatttctcac
     3121 atagaaaaca gcttgggcat ggccttatcg ggcggcgaaa gaagacgcgt tgagattgca
     3181 agagccctgg caatagaacc atcctttatc ttgcttgatg agccttttgc cggggttgat
     3241 ccaatttctg taatcgatat caaaaaaatt atcctgcatt tatgtgataa aggtataggg
     3301 gttttaatta cagatcataa cgtgagagag actttagaca tttgcgaacg cgcttatatt
     3361 gtaagtcagg gaaaaattct ttgcgaagga actcccaaag cgatacttgc caatcaacaa
     3421 gtaaggcaag tttatttggg tgaagaattt gctttgtagc ctcatttaaa agaatacatt
     3481 ttaattatac ttatgttact aattattgat aactacgatt catttacata caacctggta
     3541 cagtacttcc agagcttaaa tcaaaaagtt gaagttttta aaaatgacga aattcaaatc
     3601 gatactatca ggcaatcagc tccgcaatac ctggtcattt caccgggacc caactctcct
     3661 gatgaatcag gaatctctat agaagtcatt aaagagtttc acaagcatat tccgatattg
     3721 ggtatttgcc ttggccatca gtgcatagct caggcatttg gaggcaaaat catcccggca
     3781 ccagaaatca tgcatggtaa aacctcagaa atcacccatc attaccaagg cttattcaca
     3841 ggaataccaa atcactttca agcgacgaga tatcattctt tagccgtgga tatcatgagc
     3901 ttgcccaatt gcttttcgat agatgcgtgg gcagacgata caataatggc aatatcacat
     3961 cgtcaatttc ctctttttgg tctacaattt catcctgaag ccatattaag tcaatttggc
     4021 ttgcaaatac tcgagaattt tttacgctat gaaacccaaa cttctatttg aacaacttct
     4081 atcccgacag gatttaagct cagaccagat gcaagaggtc atccatgctt gtatgacagg
     4141 tgaatttagt gatgtgcaaa ttgcaacctt tctggcatta atgcgcatga aaggtgaaac
     4201 agtcaatgaa cttactgcag cggctcaagt catgagacaa ttagcccaca aaatagattt
     4261 gggaaatccc ctgatagata tagttggcac tggcggtgat ggaagaaata cttttaacgt
     4321 atcgacagcc tgtagttttg ttgtagctgc agcaggaata aaagttgcaa agcatggtaa
     4381 ccgttctgtt tcgagtcgta gcggtagcgc tgatctatta gaacaagccg gatttatact
     4441 caatctttct gactcacagg tacaaaactg catcaaccag tgtcaattgg ctttcttgtt
     4501 tgcgccacat tatcatcccg ccatgcaaca cgcgagagct gcaaggcaac aattaggaat
     4561 aagaacttta tttaatttac ttggtccact gattaacccg gctcaggtaa aacgacaagt
     4621 agttggtgtg ttttcaacga attggctcaa aacaatagcc actgttctgg ctaacttagg
     4681 tagtgaaaga tcattagtaa taagctctca agatggactg gatgagatta gtattgcagc
     4741 gaaaagtgaa gtagttgaat accgagatgg aaattttaag caatggttca tttctcctga
     4801 agattatggt ctaaagcatt cttctttaga tgcaatcata gtcgactcac cagagcaaag
     4861 cctgcatttg attcaatccg tactttcagg tgattctggc ccagctcgag atatagtcct
     4921 tctcaattct gccgcagcca tttattgtgc caaagatggc atatcgtttg atgcggccat
     4981 agaggaagca agaattgcca ttgatagtgg taaggcaaat ctttgtttta acaaactccg
     5041 attattgacc caaactttaa ataaagaatc aaatcatgaa tagtatcctg gaacgaatag
     5101 caaaacataa actagaagaa gttgcagttg cgaaaaaaaa taagccttta catgttctgt
     5161 caaaacaaca gccgggggaa atgcgtgact tcataactgc tttaaaatcc aatacctcac
     5221 ctgcagtcat agctgagata aaaaaggctt ctccaagcaa aggtttaatt cgaaaagatt
     5281 ttaatgttgc agaaattgcc aagatttata cccaaaatgg cgcaagatgc ttatccgttt
     5341 taacggatat tgagtttttt caaggtcatc ctgattattt ggctttagca aaatcaaaaa
     5401 ccactttgcc cgtattgcgt aaagacttta ttattgattc ttatcaaatc tatgaaagcc
     5461 ttgtcttggg tgctgattgc attttactga ttgtcgcatt attagatgat gttcaattaa
     5521 tggatttttg ccaactggct caagaactta aaatgtctgt tttggttgaa agccacacgc
     5581 aagatgaatt agaaagagca ttacgcttac ccacccccct tataggcatt aataaccgca
     5641 gcctacacaa ctttaaaaca gacattcaac tgagtatcca attaaaacaa tttgttccta
     5701 aagataaaat tattattact gaaagcggaa tcaacacccg tgaagacata aaattaatgc
     5761 aatcccatgg cattaatgcc tttttgattg gagagagttt aatgagggca gataacatcg
     5821 ggaaagcatt acagaaacta atgactgact aaataaacta ctcaaaacct ggctcaggag
     5881 tttgactcgg ctcaggctca ggcaactcgg gataaccagg ctccagaggc tctggattat
     5941 cagggtactc caaaggttgt tcagggtact ctgatggctc ctctggatac tcaattggct
     6001 gttctggtat ttcagttgta ttcaaagtat cgcctccttc tgatagttct attcaaagta
     6061 tagcaaaatc aattttagca atcattttgg taaatactct tgcccatgat acactttttc
     6121 ataaaatcca cattagaaac ctcaatgcct ccctagcgcc acaggaacag gacttacgaa
     6181 aaaccccaag gtcaaggcaa aaaaagtttt taatgaggga gtttagataa actaaatgac
     6241 cgaattaaaa acttttttta acgaagagat tggggttttt cgtaagtcct gaagaagagg
     6301 gcattaaact tttgtaaggt attttatatg aatgtagtaa ttatcactgg tgcagcaagt
     6361 ggcattggac atgccttaag tcaagtttgt cttagcaatg gaaaaactat agtcatggtt
     6421 gataagaatc aggacaaatt acatacagaa gcccaatcat tcatgactaa attctctcct
     6481 gaacatgtta tttgttttgc ctgcgacgta acccaaccat cagaagtaga acgacttgca
     6541 gaatattcct gtaaacaact aggccaagta gactggatat ttaataatgc tgggatcatt
     6601 ggcaatttac ttcctgcctg ggaactgcaa gaaaatgata ttaatcaggt aatgcaagta
     6661 aatttgcatg ggatgcttaa tataattcgc tcattcatgc cttatttatt caaacagaat
     6721 ttccgttctc atattatcaa catggcaagc ctgtatgcct tatgtacagg ctcacaaatg
     6781 gccgcctatt ccatgtcaaa acatgccgta ttagccttat ccgagtcatt atattttgat
     6841 ttaaatcgcc ttaaaaaacc tgtagatgtt tctgtagtat ttccttcttt tacagatacc
     6901 tcgttattag caagtagtgg gaaattaaac cattctccaa tccataatca attaaataac
     6961 ttgttagcac attctcgacc cgccattgaa gttgcagaac atatagtaag ggaagttgaa
     7021 caaaaaagat tttatatatt gcctgataaa gaagttaaag ggtattgtga agacagaacc
     7081 aaagccatcc tgttgcagga aaatcctcat cgtaacagcg tcgaacaatt aatgtgttct
     7141 ttattaaagc gccaatcatc agaagccaat ctgtaaattg tttgtgattt tcaaaacaga
     7201 ataagtgtaa ggcggcttat aactgatgtg agcatccatg ctttaacatt catgtactca
     7261 taacgaatct agggatagaa tttcagtctc ttatgttttt ggcctagcat ttctaacaat
     7321 tcttttctga aaatatgata atcgacctct agagcatggc gcatgtgcga tttaaatcgg
     7381 tgatggggtt tattgatttc tttggctatt tttcgatgta attgatcagg tcgtttcaaa
     7441 gtccctgcaa taatgcgagc tactattttt gattgataat cagccaaagg ccaaatgcaa
     7501 ccttgaggtt ggcataggcc aatgaaatac aaattttcaa actcggggtg catcattttt
     7561 cgatataaag ggatcgtggt agagttacta aaatcaatac aacttttatc aaaaaatgga
     7621 aaactgattt ggtaacccgt agcgaaaatg atcgtatcga actccccttg ggttctatca
     7681 gtaaaatgaa ctgtgttgcc ctcaaatctc gatatacccg aacgggggtg tattttgcca
     7741 tggcgaataa aatacaagag ttcggaatta atagtagggt gaatttccaa tggcccacaa
     7801 tcgggcttca ttaatttata cttggcatac cggccttgca agcctctaat aaccaaactg
     7861 atgaacttct gccttagcca ggatggcatc cattgtattt tagctactgc tacatccgtt
     7921 ggcttaccaa aaataaattt ggggaaaata tgataccctc tacgcatgct aatgcaagtt
     7981 ccgggagcaa cccgcgatat ttcaacagcg acatcacagg ctgaattacc cccgccaaca
     8041 accagtactc gcttgccttt aaaaattgaa gcttttttat attggtgaga atgaagtatc
     8101 tccccgtcaa atactccagg atatacaggc ataaagggat cccaatgatg accattagct
     8161 accagcaaat agtcatagca cgcttcatga attccttggg catcctcata gataacatgc
     8221 catgtattat catcaagtcg atgtaccttt tgtaccactg tgttaaaacg aatgtatcta
     8281 tctaaatgaa aatgctccac ataactctga aaatatttta aaagctggga gtgtgaagga
     8341 taatccgggt aatcaacagg catagggaaa tcttcaaact cagaccagcg ttttgaacta
     8401 atgatatgag tcgtctcata aacgctggaa tgctcatttt gctcatcata aacccaatta
     8461 ccacctattt gattgttttt ttcaaagaca gtaacattag taatgccgtg ttcttgcaaa
     8521 tttttaatgg cagcaatacc gctgggacca gcaccgatca cacaaacacg ggcagacaga
     8581 cggtccttaa gcaccctttt tatttccatc aactaacacc ttatcaactt tatccattat
     8641 catcgcttgt aaaatttcgt ctttgtcaat tgtaaaatga tttactttta tcccttttat
     8701 cgaatcaaca ttgatattat caatctttgt caattgagga tcgactgctc tgagttttaa
     8761 cccgctaaac atcggtacaa aaccaggttt atatacattc aaagcttgtt taacattagg
     8821 aggcacaata gtttgtgata ctgcatcgac agtcactaac aaagcaacag ggacacctgc
     8881 ggcattcaaa tttcgagcca ctttgatttg ttcattagcc cctaaagaat gccccactaa
     8941 aataattggc ttgtgatctt tctcttgata gtaatggtca ataatatacc gacttaccgc
     9001 tccagcgtta taccacatgg tgctatgagc cgtgatatgg tatttttcag acactttatc
     9061 acgaagtgtg tacatcccct tgctgaaaat tcccaggccg ccaagcatag tatggacttc
     9121 gccttttttt gatgacatat tttgtgcttt ggactttttg ttagggacat cagaaattgc
     9181 aggttgcttt tctttcttga tataagtatt tcgcgcgccc gtcaaatcca cacaagccgt
     9241 taaactacta acaatcaaca acaataaaaa tgatttggaa aaacgtattt gcagtttgcc
     9301 aaaatagttc atattaatat tattattgta attatttgat tttttatcat accttattct
     9361 tgtaccattt tacagaggca cctggcgggt ttaaggctat tcgcgaattc attttaatgc
     9421 cttgagaata atctgttttt catctcgtta ttttattaat tctacagaag ataaaaagcg
     9481 tccctgcccc caaggtatgt ggactatttg taatgcttgc cattgagcct cgctttccac
     9541 ttgagttgct accaccgcaa tatccatcgt tttagccatt tcattaaaat aatgtatgaa
     9601 aaactgcttg ctttcatttt catcaatatc tgtcaccaga ctcccatgca gctttaaata
     9661 atctatgttt aaatcactca aataatgcaa cggagaaaat ttaataccca cacgatctat
     9721 tccaactgtc acccccaatt ttttggcttg cttgataaag aacttggagt ttgaaaaatg
     9781 tgacaaaacg tgagcctcat taatttccag tgaaagattt ctcaagacag ctgcaggagt
     9841 gtcttccaat tgacgcaaat aaccagtgct attgacttta tttgctaaag tatcttctga
     9901 aatattaaga gcaaaatggg tatgtgttgc aatgtccatg acggttaatt cattgagcac
     9961 atactgatca ataggatagg ccaatcctaa tttctcagca actggtataa aataaccagc
    10021 gcctaactcc tccccttcct ggttgcgaat tcgaacgaag acctctttat gaaaacaatt
    10081 cttaccgtct gtaactgcct gcgcatacaa gcttattttt ttctgttgca atgagtttct
    10141 aatttcatcg ccgctgatta actgaggata tttataagta tcatgctctt tctggcaata
    10201 aaatacgccg gtttcacgag cctttttaac cgcttgatct accgtagaca acagattgga
    10261 gacaggttgg tggagaaaat aactcgcagc ccccatgtgt attttgcaaa ttttaatatc
    10321 attgatacct tggctcaaaa tttgttcaaa ctcctgacat tccttttcaa acaccaaagg
    10381 atccctttca tgactaatta aggcaaaggt cgtcccgtta attcttgcca atgtactaac
    10441 agacgattgc ttccaatagc ttttacatat tttggctaca gttaatacca attgatcccc
    10501 ttgctgatac ccttgcttct gatttaactc atccagaccg tctatgacca gcatcatgac
    10561 ataaccaggt ataaattcat cttcattatc cagaatcaaa gccaaatgct gtaaaaaata
    10621 acgacgatta ctcatccctg ttaatgaatc ttggtaaacc tgtgttctaa gtgattctgt
    10681 ttgttttaat tggtcttgaa ataaggattt gactttagta accatttgat tcatagccaa
    10741 ggtaacttgc ctcaattcag gagttttagg aatcttcgtt tctacaggaa actcatgttc
    10801 tgaaattgcc aaagcctgtg ctgtgactcg ttttaaaggt tgcaataaat attttagaaa
    10861 gccatagctt agaaccaaag caactaacgc aaaaatcaga taagcgttta ccatttctac
    10921 agcattacgc cacaaagagg cgtaaacata gctcggatca ctagcgacta aaacaactcc
    10981 tgcttgcatc cacccatcca taattaagga tgacttctca gtcgagggcc attttatcaa
    11041 gttcacaaac cattgtggta tatcgctttc ttgtggcaat tgtttcttta aaatgatcac
    11101 tttccctttt atgtcttgta ctttaattga tgaaaaatat cctctgtcaa aaacagcttt
    11161 aaccatagaa tccatggtag gtacatcatg attgattaaa gactgagata aagacaaacc
    11221 taatgatgtc gcagtatctt gagcattact ttcaagttgt tggataaaaa aattacgcgc
    11281 attattcata gtgatgaaat aagttccgat aaaaacgaat aacaacataa tcaacacgcc
    11341 tacagccatt ttcttagtta atgtcatggt ctatcacctt ttttattcca tacgtttcat
    11401 tagagcatcc cacttgccca aactttttga atcccccatc aatgaactct ctccccttgt
    11461 tttcgcaagc cataaacctt ccgcattaaa gctataaact ggtttcaaat caggccttgc
    11521 agaagctttc agaattttac tttccaaact gtcaaggatt agtggttcgg attcaggagt
    11581 tgggtaaaaa gacagcacca tatgtgcttg gtttagtgtc agagaagcag cataagtgat
    11641 tcttaactgg tcagaaggaa caccaacagc aactaatgta aagtatttta ttatggcaaa
    11701 atcttcacaa tcacctcccc catcaacaat aaattctact ggtgatttcc aataatcatc
    11761 acttgcacca gttattgggt cagtttcata attaaattga ttaaagaaag agtttacttt
    11821 ttccagttgt atttttactg gctttttttt aagtgagtca attaaattcc cccatgcggt
    11881 aaaccgtttc cgggtgtccc cctggtagga ttgcgccaat ttctgaattt tttccacact
    11941 gattaaaggc gcggcttgag tggaaaagct tgttacatag caaagtatca aaccagtcca
    12001 aatggataaa accaatctat aacgaagaga ttccaaaaaa accttctgaa atctcttagc
    12061 tataacacta agccttcttg ggcggctctt tgaaagtaac caggactcct tcaacatgag
    12121 ccaattcctt tagcctttcc attccattac ccgcatgacc tctgtattca taaggaccaa
    12181 caaaaacgga cagaagatct ttataagggc tcatgaaggc agtaaagcct aatccattta
    12241 accgtttaac caaagctacg gcattatttt tatctctgaa agagcctgct gagatatacc
    12301 ataacttggg cgttatttgc tgaggataag tagtgttttt gtctaccatc gatttagtta
    12361 aacgagtttt ctccatgttc ttaacaggat gagtgagaac cataggatta tccgtattat
    12421 tgggataagg gatctcatcc attttcctgt tcaataaaat attggtctga gccgacgaga
    12481 atacatcatt attcaccaca tttaccggca acctcaaatt caaatgtttt aataaacaac
    12541 ccattccatt caggattcga tagcgggaaa gtatctcgtc attttccgcg cgggccaatt
    12601 caatttgtga ttcataatat tcattttgag aatcgagtaa atcaaggagc gtccttttac
    12661 ccaccttaaa ttcatcttga taggccgtac gtgtttttct cgaagcggta acgtgttgct
    12721 tcaatggttg aatgcgcaag gcagaggcaa cataagcatt ccaggctaac ctgatagtct
    12781 cttttaattc cagcaatgtg cgattttttg tttcataagc ttcctggact tgataagctg
    12841 tttctcgtat acgagcggca tcagcaccac cacgaaatgc attgtaattc attcttacag
    12901 ccaccgtgtc ggagtcatta ggacctatca gaccacccaa gttcttgttt tttgagctat
    12961 tcaaaaccag attaacctca gggtaatagg ctgctctggc gacatcgtac tgagcctttg
    13021 cttctttaat atcagcgtaa cttgatttca ctgttgggtg attatctaat cctttttcta
    13081 taatcctggc taaattatta ggcaaacttt ttcttgctgg aacatgaggc acttggagat
    13141 tctctggcca tttacctact gtttttgcat aattgatctt cacctcttgc aaatcagcca
    13201 atgcacttat tttattagcc tcggcaagag ccagtcttgc attagcctga tcgacttctg
    13261 ccgttctggt aatcccggcg gtagcccgtt cttttatcat cttaaaaacc gctttatgtg
    13321 ctttaagatt ttcgatggag taagcataca aacgttcatg cagtaataca gcgaaataat
    13381 ttttggtaat ttccaatgcc aaatcttcag caataccctg tgttttccac ttttgcgcct
    13441 cagtaaggta ttggtttcgc ttcacttcat taattatccc tccaccagca aacaatctct
    13501 gccttaactc cacggccgat tccactatat tgagagtagt actgggagta tcatcgatcg
    13561 ctgcagatgt cgggttaaca ctcctttgtc gtccaaaccc accagttaca tcgatagaag
    13621 gatataaacc accctttgct ttatcaattg cctgtttcgc tgacaaacct tttgcagtat
    13681 taagtagtat atccgggtta gcgatcatgc catattgtac tgcttcataa agagtatctg
    13741 cagcaacgta ggaagaagta agcattgcaa ttaccaacag cgcctttctc attcctcatt
    13801 ccttattatt gttattttgt taattatcta tatagtaacg tataacctta aatgaaataa
    13861 catctcacta caatataatt ttatttcttt aattagttat tgttgttaac aaccttttga
    13921 tttgatggga tttttaccac catgagtagt caattgccaa tactgaccgt gagtcagctg
    13981 aatagacaag tgaaaggttt tctcgagaat gaaataggcc ttgtgcatgt cgaaggcgaa
    14041 atctctaact taagcaaacc ttcttcagga cattactatt tcaccctgaa agacagcaca
    14101 gcgcaaattc gttgcgcttt ttttaaaaat cgacattcca attctctgct tagaaatttt
    14161 aatgatggtc aacaaattgt tgccactggc aaattaagtc tgtatgaagc ccggggagaa
    14221 tatcagctta ttgtcgaaga gatagttaaa gcaggaatgg ggattctcta tcaacgattt
    14281 gaagagttaa aaatcaaact ggccagcgaa ggcttattca atcctgagag aaaaaaaccg
    14341 ctgcccagaa tacccgaaac gattggcatc attacatcac ccaccggagc agctatccag
    14401 gacattctct caactctggc ccgtcgattc ccaatagcca gaattattat ttatccaagt
    14461 gaagttcagg ggcaaaccgc ccctcaacaa ctagtcaagg ccttaaaact ggctaatgcc
    14521 cataaacgat gccaggttct catcctggcg cgcggcggtg gcagcattga agacttgtgg
    14581 gcctttaatg atgaatacct tgctcgacaa attgcaatca gcgaaattcc tgttgtttcc
    14641 ggtattggtc atgaaacaga tttcacaatt gctgattttg tagcagatta tcgtgcagaa
    14701 acgcctactg ctgccgctac ggctgttact ccaaactgca tcgaattatt caatattctt
    14761 gatacggcta tttatcgatt gcatgatgcc atcatcagac tagttaaagg gttgcaatta
    14821 aagctcaatc atttgataga taaaattgcc tcacccaggc aaactatctc aacatattgg
    14881 caaaccctgg attatctgga gagacagtta atatctgcta tgactcaatt tattaactta
    14941 aatattaata aggtgaatat tttttcaaca caattgcaag ctaacaatcc caaaacacaa
    15001 attgaacgaa ctaaaaccca actacgacaa ttaataatgc aattaaacca agagatccgg
    15061 atacaagtaa accaattaaa aaatcaacta tccactaatc tttccacttt gcatgctgta
    15121 agccccttgg ctacactaga tagaggttat gctattgtga gtaaaaacca aagaatactc
    15181 tttgctgccc aacaggcaca aattggagat actatcgatg tccgtttagc aaaaggaagt
    15241 cttgcttgtg aggttactca aattaaggat tgactaaaat catgaagaat ctattacatt
    15301 cacttgcctg tctattgata ttaataagct cagcaaaact atcctatggt ctggctctac
    15361 cagaaaatca ttctgtcaat gggggactga ctattattcc aattgatttc aatcagaagc
    15421 ctgaagccta tttcgagggg aaacgcatcc cggttcttcc cagtacccaa ccccggcaat
    15481 ggttattaat cgttgcaatc ccgcttaaaa ataccaactc tgttcaatac atcaacgtga
    15541 caaaacccat caaagcatct attccctttc atgttagtga aaaattttac agtacccaat
    15601 ttttaaatat aaaagatatc agtaaagtgg atcctcaacc cgaagattta atccgtattg
    15661 aaaaagaaac caaaaaatta acccaaattt ttgctaatta ctccaatacc aatccatttg
    15721 agaaacaatt cacagcacca ctgagaggac ctataaccag tttatttggc ctgaagagag
    15781 tttataataa acagccaaga gatccgcatt ctggattgga catcgccgct gacgagggga
    15841 aacctgtgta tgctgtaaat cagggagttg tagcagatac cggtgattat tttttcacag
    15901 gcaatacggt tatccttgat catggcatgg gagtattctc ggtttatgct catcttagca
    15961 aaattctggt taaaacagga gaaacaatca aacaaggcca agagcttgga ttagttggta
    16021 tgacaggaag agtaacaggc ccccatctcc actggactat ggtagttaac caaactttgg
    16081 ttgagccttt actttttgtg ccgtttcgca aaatcacagt tacaccagtt ataccagata
    16141 aaaaatcaaa caaatcaaca caagatgcaa cggcaaaatc atgattaacg aattaacgac
    16201 cagtttgaat gtgattgtca cccagacaca aaataacctg aaaattttgg gagtaatact
    16261 actaattccc tggtcaatat tttttattag tcattttata agtaataaaa ttttgcttct
    16321 tggcattatc ccaaggcata tattgggcct cccggggata ctattagcac ctttacttca
    16381 cgctaatttt aatcacttat tttttaactc tatcccttta ctggttttaa gtaattttat
    16441 tctgattaat ggagttaact attatttgat tgtcacagtg atgataactc tttttagcgg
    16501 gacactgatt tggtgttttg ctaaacgagg tatacacatt ggcg
//
LOCUS       DAXLDT010000064        16480 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXLDT010000064 DAXLDT010000000
VERSION     DAXLDT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.064"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..828)
                     /locus_tag="ACF2SP_001660"
     CDS             complement(<1..828)
                     /locus_tag="ACF2SP_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HFD2359448.1"
                     /translation="MKLKEIRTIIYNYGEEEILKHILYESKEPILIKIPDFTSQFSLD
                     YFEQLTQAKTTYCTFENKRCVDFQAGNFAEVIQQIKSNKPIRIFGQILSRERSKEIEK
                     HVPLWQKIPFRPRYFRETKKVAYFFGGKGAVSEMHFDREHCCNLHLCLSGKKQVLLFT
                     RKQSDNLYKLPFIGDSLIDFTQPLDVLIQQYPRLNQAEGYQVLLEKGDMLFMPRNCWH
                     YTEYLDASSAATYVFYPNKFFQYYGYFTGNFFLGYKEESGFKIYRWPFFEKFSRRYAL
                     "
     gene            complement(1078..1854)
                     /locus_tag="ACF2SP_001661"
     CDS             complement(1078..1854)
                     /locus_tag="ACF2SP_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFD2359449.1"
                     /translation="MSSQTNVYPANKLNLWPLLILALAIFIPMTSYSASDWQELTPGI
                     EYQDLEGGLLNPWSHIHVFRIDLNKNQMALVTAKNLAQKNASVDQFAEHSKALLSING
                     GFFDHEFNPLGLRINNKKQENPLKRISWWGIFYVKDNKPRITNIRNFHYDSNIDFAIQ
                     SGPRLLIRGNIPSLKAGVADRTALGITDDGKVIILVTTNAAMSTRQLAQIMRSPPLSC
                     SDAINLDGGSSSQLYAKIDSFQLNVHGFSNVSDAIIVKKI"
     gene            1900..3258
                     /gene="pmbA"
                     /locus_tag="ACF2SP_001662"
     CDS             1900..3258
                     /gene="pmbA"
                     /locus_tag="ACF2SP_001662"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFD2359450.1"
                     /translation="MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNY
                     DRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIA
                     KVSAEEPCFGLADKELMTKNHPDLDLYHSWDITPQQAIDLALKCESHALSLDKRITNS
                     DGVNVSSYESHHGYANTHGGEGFIHSTRHSISCSVIAKEGDEMQRDYDYTTARNAENL
                     VSADLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNS
                     FLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSY
                     SARKMGLKTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSR
                     GASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVA
                     GK"
     gene            complement(3422..3643)
                     /gene="infA"
                     /locus_tag="ACF2SP_001663"
     CDS             complement(3422..3643)
                     /gene="infA"
                     /locus_tag="ACF2SP_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFD2359451.1"
                     /translation="MAKEDHIEMAGTVIDTLPNTMFRVELENGHIVTAHISGRMRKNY
                     IRILTGDKVKVELTPYDLSKGRIIFRDKG"
     gene            complement(3775..4146)
                     /locus_tag="ACF2SP_001664"
     CDS             complement(3775..4146)
                     /locus_tag="ACF2SP_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFD2359452.1"
                     /translation="MKQHSQGFLKLVAEAKKRINEISPQILKSKIDNNEKLIIIDVRE
                     TNEWETGKIPNAIHLSKGIIERDIEKIITDNQSNIVVYCSGGYRCALVADSLQKMRYT
                     NVYSLDTGLQGWLDAGYSLVK"
     gene            complement(4210..4878)
                     /gene="aat"
                     /locus_tag="ACF2SP_001665"
     CDS             complement(4210..4878)
                     /gene="aat"
                     /locus_tag="ACF2SP_001665"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFD2359453.1"
                     /translation="MAYDSDYTFPDPETSDKQGLLAIGGVLTPKRVLQAYSQGIFPWY
                     EPGNPVLWWSPNPRLILIPNEFKISRSLKKTLKKPFKLTVDTAFQRVISYCATCSDRT
                     NKTWITSEMIETYTQLHEMGYAHSFEIWDGSELVGGLYGISLGHAFFGESMFHTITDA
                     SKVALHFLCSIMQSWNFDFIDCQLPTLHLMRLGAKIISRKEFLHMLQETLKYPDKKGN
                     WSVD"
     gene            complement(4881..5831)
                     /gene="trxB"
                     /locus_tag="ACF2SP_001666"
     CDS             complement(4881..5831)
                     /gene="trxB"
                     /locus_tag="ACF2SP_001666"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFD2359454.1"
                     /translation="MSISNHHRLIILGSGPAGYTAAVYAARANLNPVLITGMQPGGQL
                     TTTTDVDNWPGDIEGLQGPALMERMQKHAERFDTQVIFDHIVKADLAQAPFTLQGDSA
                     TYTCDALIIATGASARYLGLPSETAYQGRGVSACATCDGFFYRNKSVCVIGGGNTAVE
                     EALYLSNLASSVTLIHRRDSLRAEKILQNKLFEKAHSGNIKIVWNSTLDEVLGDEKKV
                     TGAMIRNLKTGETDQLAMDGIFIAIGHTPNTAIFQDQLAMRDGYILIKSGLDGMATST
                     SVPGVFACGDVADHVYRQAITSAGFGCMAALDAEKYLDSI"
     gene            5904..8288
                     /locus_tag="ACF2SP_001667"
     CDS             5904..8288
                     /locus_tag="ACF2SP_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFD2359455.1"
                     /translation="MAKQHSGKQAGIPKKSMPSFIIKRLCEGSFILILTGALFVLLSL
                     FTYSTNDPGWSHASRSGMSVSNSGGQVGAYIADALYFAFGYFAFLLPIAFVYIAWAIL
                     KDFRSFKELDRMVLLLRTVGFILMVSGGCGLLSINHRFEAVDTIHSSGGIIGQTVGNG
                     WCQMLNMEGATLLLLAMFLVGTTWLTGLSWIKAIELIGYYTLNLFTFLDKFIRKGMQI
                     ISENFNKEKLKTPLIKTEQLPKPDNEKKKSVPKLFQDKKDKEQEKATPVLIASEEKPE
                     IVKSTNEFKEIRPPKTITPGSLPSLSLLDKGQPGKPMGGYTHEELESLSRDVEQHLLD
                     FGIQADVVAVHPGPVVTRFELQLAAGVKVSKLTALAKDLARSLSVISVRVVEVIPGKT
                     VVGLELPNHSRQVVRLSDVLSADVYQQAHSPLSLALGVDIGGHPMVVDLAKMPHLLVA
                     GTTGSGKSVGINAMILSILFKASPEQVRLIMVDPKMLELSVYDGIPHLLTPVVTDMKE
                     AASALRWCVEEMERRYRLMAALGVRNLAGYNTKITEAAVNGQPLLNPLWKPVDSMDET
                     APELQALPYVVVVIDELADMMMVVGKKVEQLIARIAQKARAAGIHMILATQRPSVDVL
                     TGLIKSNIPTRISFQVSSKIDSRTILDQQGAEQLLGHGDMLYLAPGSGAPLRVHGAFV
                     DDKEVHRIADDWRSRGEPDYVDDILKMVNENGDGAFDDDNGGQSVEDDDPLYDQAVEF
                     VIQTRKASISAVQRRLKIGYNRAARMIEEMERTGIVGPLDGGYRDVLVTSVTED"
     gene            8291..8899
                     /gene="lolA"
                     /locus_tag="ACF2SP_001668"
     CDS             8291..8899
                     /gene="lolA"
                     /locus_tag="ACF2SP_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFD2359456.1"
                     /translation="MNKLFLILLLIFSHEVFSQTSAEVLQSKLNAIQTMTANFSQIVK
                     AKNHEVSRSSGSMALQRPGRFRWQTKDPLEQLIVADGQKMWIYDVDLEQVTVKNQEKG
                     LGGTAALFLSGYDETLTHDFDVSEKQKGKLTVFDLKSKSAKENFQRIKLIFSQSALIG
                     LELYDQLGQITDVKLVQIKSNPKLPAKLFQFKPPKGVDVVKQ"
     gene            8915..10219
                     /locus_tag="ACF2SP_001669"
     CDS             8915..10219
                     /locus_tag="ACF2SP_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFD2359457.1"
                     /translation="MSLFNKEPDPPLAESLRPKHIDEVIGQSHLLGEGKPLRLCFMGS
                     KLHSMILWGPPGVGKTTIARLTAQAFDCEWIALSAVFSGVKDIRAAIEKAQEYLIHDK
                     QTILFIDEIHRFNKAQQDALLPYTESGLITFIGATTENPSFEVNPALLSRAQVYVLKL
                     LSEQELKLLFLRAHQRALSSLQFTEEAMDFLISCADGDARRLLNLLEQTKTACLTIKT
                     AVVDVELLHNMIVQSGRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLCRMLDGGVD
                     PYYLARRIIRMSWEDIGLADPKAIQIANDAAATYERLGSPEGELALAQAVIYLSVAAK
                     SNAGYVAFNQAMEFIKRDKSREVPIHLRNAPTQLMKKLGYGHEYRYAHDEPHGYAAGE
                     CYLPEGMPEVNWYNPVQRGLETKIAEKLAFLRQLDKDVKKKV"
     gene            10518..11549
                     /locus_tag="ACF2SP_001670"
     CDS             10518..11549
                     /locus_tag="ACF2SP_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD2359458.1"
                     /translation="MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVW
                     LNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLADGRRVHVAISSLESLPGIL
                     ITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDH
                     RIKNWIHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYA
                     AVLHVNNHLIARESSIHGESLIGAILNLIINALQSEATQIDLTLASMDDSGIQISVED
                     NGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIY
                     LPCLNRNVG"
     gene            11573..12886
                     /gene="fleR"
                     /locus_tag="ACF2SP_001671"
     CDS             11573..12886
                     /gene="fleR"
                     /locus_tag="ACF2SP_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor FleR"
                     /protein_id="HFD2359459.1"
                     /translation="MSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
                     NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQK
                     PFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGT
                     GKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQ
                     AQGGTLLLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEV
                     LAGRFREDLYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQK
                     AMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEAKTKGTSKNLQI
                     HEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV"
     gene            12901..13215
                     /gene="fliE"
                     /locus_tag="ACF2SP_001672"
     CDS             12901..13215
                     /gene="fliE"
                     /locus_tag="ACF2SP_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFD2359460.1"
                     /translation="MSDINTVNLINQMKTLAAQAEGIKLDSGLNHTSFSSVFQQALNQ
                     VNELSQTADSLKTRFEIGDPNVSLGEVMIASQKSNLGFEATLRVRNKLVQAYQDIMNM
                     PV"
     gene            13388..15022
                     /gene="fliF"
                     /locus_tag="ACF2SP_001673"
     CDS             13388..15022
                     /gene="fliF"
                     /locus_tag="ACF2SP_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFD2359461.1"
                     /translation="MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSR
                     DPSYVPLYSQLNAQDSSEIMTILERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGL
                     PRDTAIGHDLLGSSGGVFNTSQFMENARYKQALEAELARTISKFNNIKSARVHLAIPR
                     ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSAGRVTVVDQDGQL
                     LNEGSGYNLFSETERFLDYRQNLEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYE
                     QTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATP
                     NNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMND
                     KTKKLEKKPLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFW
                     QKDSFWSVIKQIGGALFILAILFGVLRPMLKTLASNKDIDQATDNQDTGDFTLDGKIS
                     FRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG"
     gene            15025..16014
                     /gene="fliG"
                     /locus_tag="ACF2SP_001674"
     CDS             15025..16014
                     /gene="fliG"
                     /locus_tag="ACF2SP_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFD2359462.1"
                     /translation="MDGIERAAILLLSMGEKNASEVLKHLEPRQVQKVGMAMSTMNSV
                     SKAKMQNVLIDFISAIEDQTSLTVDTEHYLRTVLIEALGEEKAIPFIDRILVSNKDSG
                     LNKLKWLDGRLIAEVIRNEHPQIIATILIHLDSEQSAEVVSYFSAEKRAEVLLRMCNI
                     DTVKPEAISELGHVIEKQLSGQTTGKSSSVGGIKTVADVINFLDGAMEEEVLERIKDW
                     DEELSEKIRDKMFVFENIKDMDDRSVQTLLREVTSDQLKLALKGTTETTKEKIYSNMS
                     KRAADLLRDDIEVQGPVRVSDVERAQRDILAIARNLAEDGKISLGAKGGDELI"
     gene            16156..>16480
                     /locus_tag="ACF2SP_001675"
     CDS             16156..>16480
                     /locus_tag="ACF2SP_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFD2359463.1"
                     /translation="MSNEFEPYNKEVKKIEFTTWKYQVSNDAENSPSDEEELINELEV
                     VKQEAFQKGYADGFQQAQEEINAIKAELSKWLDLIQKPVQILDEQLTQEIIQTIIWLS
                     QHCVAV"
BASE COUNT         5193 a         2863 c         3553 g         4871 t
ORIGIN      
        1 taatgcatag cgacggctga atttctcaaa aaaaggccat ctataaattt taaaaccgga
       61 ttcttcttta taccccaaaa aaaaattgcc ggtaaaatat ccataatatt ggaaaaattt
      121 gttaggataa aacacataag tagcggcaga tgacgcatca agatactctg tgtaatgcca
      181 acaatttcta ggcatgaata acatatcgcc tttctcaagt agaacctgat atccttcagc
      241 ctgattcagt ctggggtatt gttgaattaa aacatccagg ggttgagtga agtcaatcaa
      301 agaatcccca ataaagggga gtttgtaaag gttgtcactt tgttttctgg taaacagcaa
      361 tacttgtttt ttcccactca aacataaatg aagattgcag caatgttctc ggtcaaaatg
      421 catttctgag acagcgcctt tacctccaaa aaaataagcc acttttttgg tttctctaaa
      481 ataccttggc ctgaaaggaa ttttttgcca aagtggcaca tgcttttcta tttccttaga
      541 gcgttctcta gacaaaattt gcccaaaaat acgaatgggt ttgttgcttt ttatttgttg
      601 gatgacttca gcaaaattgc ccgcttggaa atcaacacat cgcttgtttt caaaggtaca
      661 ataagtggtt ttggcctgag tgagttgttc aaaataatcg agtgaaaatt ggctggtaaa
      721 atcaggtatt ttaattaaga taggttcttt ggattcatac aagatatgct taaggatttc
      781 ttcctcacca tagttgtaga tgattgttct gatttctttt aatttcatta aattacctgt
      841 tgaatggagt aatttataaa tatttttcat ctcaataatc tatattatat cattaaaaaa
      901 atcatgactt gattgtgatt tatgtatacg aatgatatag cagattcggg attacggatc
      961 tcgcgaaaac aaaggtggaa tttgaaaagg agattctgca cgtggctgga acgcatgaat
     1021 taagtactat aaggggtttg aatccaatca atgataaggg gcttgactcg aatgccccta
     1081 aattttctta actatgatcg catcactgac attagaaaaa ccatgtacat tgagctgaaa
     1141 gctgtcaatc ttggcatata attggctgga acttccacca tccaaattga ttgcgtcact
     1201 acaggataat ggaggcgacc tcataatttg agctaattgt ctggttgaca tggcggcatt
     1261 tgttgtaacc aatataatca ctttgccatc gtccgtgatc cccaatgctg ttctgtcagc
     1321 aaccccggct ttaagagaag ggatgtttcc tctgattaat aatctggggc cgctttgaat
     1381 agcaaaatca atgttactgt cataatgaaa attgcgtatg ttagtaatac gaggtttatt
     1441 atccttaaca taaaaaatgc cccaccagct aatgcgtttt aatggatttt cctgtttttt
     1501 gttgttaatc ctaagcccca gaggattgaa ttcatgatca aaaaatccgc catttatgct
     1561 aagtaaagct ttgctgtgtt cagcaaattg atcaacggat gcattttttt gagcaaggtt
     1621 tttggctgtc accaatgcca tctgattttt attaagatcg atacgaaata catgaatatg
     1681 agaccatgga tttaacaagc ctccttctag atcctgatat tcaatacctg gagtaagttc
     1741 ttgccaatca ctggcagagt aggacgtcat agggatgaaa atggcgagtg ctaaaataag
     1801 caagggccat aaattcaatt tgtttgcagg ataaacattg gtttgcgaag acatgttatt
     1861 gtatccttat gtacatcatt caacaccacg gaatctcata tgcaaattaa accgcaaaat
     1921 aacaatagtg aagtatataa gtcaacaaca gatttaaatc gagtgataaa agatgtcttg
     1981 gaacttgcca aaaaagaagg tgccactgat gcagcagtgg ctgtaaatta tgatcgaggt
     2041 ttttcagtcg atgtgagaat gggggaagtg gagacagtcg cttttagcga agataaagga
     2101 gttggtttaa ctgtatacat tgggcagcgc aaagggggag caagcagtac agacacatct
     2161 cctgaagcat tacagtcttt ggttaaagca gcctgtgaga tagcaaaggt tagtgctgaa
     2221 gaaccatgct ttggattagc tgataaggaa ttgatgacaa agaatcatcc agatcttgat
     2281 ttgtatcact cctgggacat taccccacaa caagctatag atttggcttt aaaatgcgaa
     2341 tcacatgcct tgtctttaga taaacggatt actaattcag atggtgttaa tgtttcttcc
     2401 tatgaatctc atcacggtta tgccaatacc catggtggag aggggtttat tcatagtact
     2461 cgacatagta taagttgctc ggtaatcgcc aaagaaggtg atgaaatgca aagagactat
     2521 gattatacga cggccagaaa tgcggaaaat ttagtctcag ctgatttgat agcagctaaa
     2581 gctgtcgaaa gggcattaag tcgtctggga tcaaaacaaa ttaagacaca aatgacaccg
     2641 gttatttttt cttcaagagt ttccagtgga ttattgggta gctttgtgag cgctattagt
     2701 ggttcaaatt tatacagaaa aaattccttt ttgcttgatt ccattggaca acaattgttt
     2761 ccggagtttg ttcatattta cgaacagcca catttaaaag gtgctttggg gagcgcccct
     2821 tttgatgggg aaggtgtgcc tacgcgaccc aatgtattag ttgaaaaggg gcgtttgctt
     2881 caatatgttc ttggcagtta ttcggcgcga aaaatgggtt taaagacaac agcaaatagt
     2941 gatggcgttc ataatttaac cattgatcct acttgcgggg atcttggtga ccttttgaaa
     3001 atgatgcgga aagggttgct ggttacagaa ctaatgggcc agggtgtgaa tggtattacc
     3061 ggagattatt cacgtggggc aagtggatac tgggttgagg atggggtcat acaatatccg
     3121 gttgatcaaa ttactattgc aggaaatctt aaagaaatgt accggatgat attggcagta
     3181 ggcagtgaca ttaatccgaa tatttccatg cgctgtggct ctattttaat agaaaatatg
     3241 atggttgccg ggaaataaaa taccccaaac catcgaggtt aaccacttgc tctgtaatct
     3301 tggtgatagt tctctggaac attagctata tacctggctg cttgcagcca gctttttcta
     3361 tattccgtca gattttcttt aatgaaacag agaacagcta tagaaattgg atgaaacgca
     3421 tttagccttt atcgcgaaaa atgatacgac cctttgaaag atcgtaagga gtcagctcta
     3481 ccttaacctt atccccagtc agaattctga tataattttt cctcatccga cctgaaatgt
     3541 gagccgtaac gatatgtcca ttttccaact caacgcgaaa catggtgttg ggtagtgtat
     3601 ctatgacagt accagccatt tcaatgtgat cttcttttgc catatgtctc tcaaatgatg
     3661 tatcaaattt ttaaaagtga aatagtgcac taaattgcgg aaaatggcaa tccagtcttg
     3721 caattacatt gttcaaagat atagcccaag gctcaattat tgggagatat tcaattattt
     3781 taccaggctg tatccagcat ctagccatcc ttgcaatcca gtatccagag aatatacatt
     3841 ggtatatctc attttttgta gactatcggc taccaaagcg cagcgataac ccccgctgca
     3901 ataaactaca atattgcttt gattatctgt gataattttt tcaatgtcac gctcaataat
     3961 ccctttactt aaatgaattg cgttcgggat tttacctgtt tcccattcat ttgtttcacg
     4021 aacatctatt ataattaact tttcattgtt atcgatttta ctttttaaaa tttgaggact
     4081 aatctcgttt atacgttttt ttgcttccgc taccagtttt aaaaaacctt gtgaatgctg
     4141 cttcatgctt attctcactc tatttagttt taaaagataa acggttattg ataaaaagat
     4201 ttaattaatt taatcaacag accaatttcc cttcttgtca gggtatttta aagtttcttg
     4261 taacatatga agaaattctt ttcgacttat tatttttgcg ccaaggcgca ttaaatgtaa
     4321 ggttggcaac tggcaatcaa taaaatcaaa attccatgat tgcataatgc tacataaaaa
     4381 atgcaaggcg actttggagg catcggttat tgtatgaaac atcgattcac caaaaaaagc
     4441 atgtccaagg ctgattccgt aaagtccacc tacaagttcg gaaccgtccc agatttcaaa
     4501 ggaatgcgca tatcccatct catgtaattg ggtgtatgtt tctatcattt cactagtgat
     4561 ccaggtctta ttagtcctgt ctgaacaagt tgcacagtat gaaatgactc gttgaaaagc
     4621 agtatcgaca gtcagtttaa aaggtttttt cagagttttt tttaaacttc tggatatttt
     4681 aaattcgtta ggtattaaga tcaggcgcgg attaggtgac caccaaagaa cagggttacc
     4741 gggctcatac caagggaaaa ttccttggga gtaggcttgc aaaactcgct tgggggtcaa
     4801 aacaccccca attgctaata aaccctgttt atcactggtt tccgggtcag gaaaagtata
     4861 gtcgctatcg taagccaatt ttatattgaa tccaggtact tttcagcatc cagggctgcc
     4921 atgcacccaa atcctgctga tgtaatggcc tgacgataaa catgatcagc aacgtcgcca
     4981 caagcaaata ctccaggaac agaggtgctt gttgccattc catcaagtcc tgacttaatc
     5041 aagatataac cgtctctcat agccagctga tcttgaaata ttgcagtatt tggtgtgtgt
     5101 ccaatggcta taaaaatacc atccattgcc agttgatcag tttcgccagt tttaaggtta
     5161 cgaatcatgg ctcctgtaac ttttttctca tcgcccagta cttcgtcaag ggttgagttc
     5221 catactattt tgatgttacc ggaatgcgct ttctcaaaga gtttattttg caatatcttt
     5281 tctgcacgta agctgtctcg tctatgaatc agagtaacgg aagatgccaa atttgataaa
     5341 taaagtgctt cttcaacggc agtattgcct cctccaataa cgcaaacact tttattgcga
     5401 taaaaaaatc catcgcaagt agcacatgct gagacacctc gcccctggta ggccgtttct
     5461 gagggtaatc ccaaataacg agcagaggct ccggttgcaa taatcaaggc atcgcaggtg
     5521 taagtagcac tatccccttg tagtgtaaag ggagcttgcg ctaaatcagc tttgacaata
     5581 tgatcgaata tgacttgagt atcaaagcgt tccgcgtgtt tttgcatacg ctccattaga
     5641 gcggggcctt gtaatccttc aatatctccg ggccagttgt ctacatcagt cgtggtcgtt
     5701 aattgtccac caggctgcat tccggtgatc aaaacgggat tcagattggc tcttgcggca
     5761 tagactgcag cagtgtaacc tgcaggacct gaaccgagta tgattaagcg atggtgattg
     5821 cttatgctca ttgtctctgc cttccttatt ttttgtgtta agatggcaaa tattatgaca
     5881 aaaataaaaa cattaaaata cctatggcaa aacaacattc gggaaaacag gctggaatcc
     5941 ctaaaaaatc catgccgagc tttataataa aacgcttgtg tgaggggagt tttatattga
     6001 ttttgacagg agctcttttt gttctcttgt ctttatttac ctattcaacc aatgatccag
     6061 gctggtctca tgcctcccgt tctggtatgt ccgtttcaaa ctcaggggga caagttggtg
     6121 cgtatattgc cgatgcgctt tattttgcat ttggatattt tgctttctta ttaccaatag
     6181 catttgttta cattgcctgg gccattttga aagattttcg ctcttttaaa gagctggata
     6241 gaatggttct gttactgaga acagttggtt ttatcctcat ggtttctggt ggttgtggct
     6301 tattgagtat aaatcatcgc tttgaagcag tagataccat tcacagctca ggaggtatta
     6361 tcggacaaac agttggaaat ggttggtgtc aaatgcttaa catggaagga gccactttac
     6421 ttttattagc aatgtttctt gtgggaacaa cctggctgac tggattatcc tggataaagg
     6481 caattgagtt aatcgggtat tacacattaa atcttttcac atttcttgat aaattcatca
     6541 gaaaaggaat gcaaattatt tctgaaaatt ttaacaaaga gaaattaaaa acaccattga
     6601 tcaagaccga acaactccct aaacctgata atgaaaaaaa gaaatctgtt cctaaattat
     6661 tccaggacaa gaaagataaa gagcaagaaa aagcaacacc tgttttaatt gcaagtgaag
     6721 aaaaacctga aattgtcaaa tctaccaatg agttcaagga aattcgtcct cctaaaacaa
     6781 taacccctgg atctcttccc agtctgtcgt tgttagataa aggtcaacca gggaagccga
     6841 tgggaggtta tacacatgag gaattggaaa gtctttcaag agatgtagaa caacacttgc
     6901 ttgattttgg tatccaggca gatgttgtag ccgttcaccc agggccggtg gtaacccgat
     6961 ttgaattgca attggcagca ggggtcaaag tgagcaaact gaccgctttg gcaaaggatt
     7021 tggcccgttc attatcagtt atttctgttc gagttgtaga ggttattcct ggtaaaactg
     7081 ttgtgggcct tgaattacct aatcattcac gtcaagtggt caggctatct gatgttttat
     7141 ctgcagatgt gtatcagcaa gctcattctc ccttatcttt agccttggga gttgatattg
     7201 gtgggcaccc tatggtggtt gatttggcaa aaatgcctca tctactggtt gcaggaacaa
     7261 ctggttctgg taaatcagtt ggtattaatg ccatgatttt aagtattctt tttaaagcaa
     7321 gtccagagca agtgcgttta attatggttg atcccaaaat gttggaatta tcagtttatg
     7381 atgggattcc acatttgctg actcctgttg ttaccgacat gaaggaggca gcgagcgcac
     7441 tgcgttggtg tgtagaggaa atggaaaggc gttataggtt aatggccgca ttgggtgtta
     7501 ggaatttggc agggtataac accaaaatta cggaagcggc agttaatggc cagccattgt
     7561 taaatccatt atggaaaccc gtcgactcta tggatgaaac agcaccagag ttgcaagcac
     7621 tgccttatgt agttgttgta attgatgaac ttgctgacat gatgatggtt gttggcaaga
     7681 aagtggagca attgattgct cgtattgcac aaaaagcaag agcagcagga atccatatga
     7741 ttcttgcgac acaaagacct tcagtggatg tattaacagg tttaattaaa tccaatattc
     7801 caacaagaat atcatttcag gtgtcttcaa aaattgactc acgcactata cttgaccaac
     7861 aaggagccga acaattgctc ggccatggtg atatgttata tctggcgcct ggaagtggag
     7921 ctcctttacg tgtgcacggt gcttttgtcg atgataagga agttcatcgt attgctgatg
     7981 actggcggtc aaggggtgaa cctgattatg ttgatgacat ccttaaaatg gtaaatgaaa
     8041 atggggatgg cgctttcgat gatgacaatg gagggcaatc cgtagaagac gatgatcctc
     8101 tttatgatca ggcggtagag tttgtaattc agacacgcaa agctagtatt tctgcggtac
     8161 agagaagatt aaaaatcggt tataatcgcg ctgccagaat gatagaggaa atggagagga
     8221 caggaatagt cggtccattg gatggtggtt atcgtgacgt attagtaaca tcagtgactg
     8281 aggactgaat atgaataaat tgtttctcat tttgttgctc attttttcgc atgaagtgtt
     8341 tagtcaaact tctgctgaag tactgcagag taagttaaat gctatacaaa ccatgactgc
     8401 aaattttagt caaattgtaa aggcaaagaa tcatgaagtg tcacgttctt caggttccat
     8461 ggcattgcaa cgtcccggac gatttcgctg gcaaacaaaa gatccattgg aacaattaat
     8521 tgttgctgac ggccaaaaaa tgtggattta tgatgtggac cttgaacaag tcacagtcaa
     8581 aaatcaggaa aagggactag gtggtactgc tgcattgttt ttgagcggct atgatgaaac
     8641 ccttacccat gatttcgatg tcagtgaaaa acaaaaaggg aaattgaccg tttttgattt
     8701 gaaatccaaa tctgcaaaag aaaattttca aagaattaag ttaattttta gtcaaagtgc
     8761 tttgattggt ctggagttat atgatcagct aggccaaatt actgatgtta aattagtgca
     8821 gattaagtca aatcctaaat tacctgccaa attgttccaa ttcaagcccc ctaaaggtgt
     8881 ggatgtggta aaacaataat tttaatgttg atttatgagc ctattcaata aagaacctga
     8941 tccaccattg gctgaaagtt taagacctaa gcatatcgat gaggtcatag gtcaaagtca
     9001 tttgcttgga gagggtaaac ccctaagact ttgttttatg ggctccaaat tgcattctat
     9061 gatcttatgg gggccaccag gggtcggtaa gactactata gcaagattaa ccgcacaggc
     9121 ttttgattgt gaatggatag cattatcagc tgtgttttcg ggtgttaagg atattcgcgc
     9181 tgcgattgag aaagcgcagg aatatttaat tcatgataaa cagactatct tgtttatcga
     9241 tgaaattcat agattcaata aagcccagca agatgcctta ttaccttata cagaatcagg
     9301 tttaatcact ttcattgggg caactacaga aaatccgtct tttgaggtta atccagcgtt
     9361 attatcacgg gcgcaagttt atgttttaaa actgttatcg gagcaggagt taaaactctt
     9421 atttcttagg gctcaccaaa gagctttatc ttctctgcaa tttaccgagg aagccatgga
     9481 ttttttaatt tcttgtgctg atggagatgc cagacgatta ctcaatttac ttgagcaaac
     9541 aaaaacagcc tgcctaacaa ttaaaacagc agttgttgat gtagagcttt tgcataatat
     9601 gatagtacaa agtggtcgac gttttgataa aggtggagaa gcgttttacg atcagatttc
     9661 tgctttacat aagtcggtac gaggctctaa tcctgatgcg gcattatatt ggctatgtcg
     9721 aatgctggat ggtggtgtgg atccatatta tttagccaga cgtattatta gaatgtcatg
     9781 ggaagacatt gggttagcag atccgaaagc aattcaaatc gctaatgatg ctgctgcaac
     9841 ttatgagagg cttggctcac ccgagggcga gcttgctttg gcacaagctg taatttattt
     9901 gtctgttgct gccaagagca atgcgggata tgtcgcattt aatcaagcta tggaatttat
     9961 aaaaagagac aagtccagag aagtacccat tcacttgagg aacgctccta ctcaattaat
    10021 gaaaaaacta ggttatggtc atgaatatcg atatgctcat gatgaaccac acggctatgc
    10081 ggctggtgaa tgttatttac ctgaaggaat gccagaagta aattggtata acccggtgca
    10141 aagaggttta gaaactaaaa ttgcagaaaa gctcgctttt ctgcgccaat tagataaaga
    10201 tgtgaaaaag aaagtttgat ttgttctctg tttaagccag gttaggtttg tctcccaatt
    10261 tatactaaag aagaagtcca atcgacattg gtatccggtg aagtataaag agtatgtcaa
    10321 aattctgact gtaggaagca tggatgattc tttaaaatct ccttaacccc atttacaatg
    10381 atttaaaaat tacaaattgt ttatattttt aaatacattc aaaaaaattt ggttttcaat
    10441 tggttaatta attgatttca aatacatttt aattttggcc tgaactttgc tagagacatt
    10501 tcataataat aattaaaatg aatgacatgc attattcaaa ttatcaaact tgtgcagtca
    10561 aaccacagat agaattatct gaatgggaca ttttgtccag tttgcctacc gccttggtaa
    10621 ttttgaatgc ccaaggcaaa gtggtgtggt taaatccttc agctgaagcg atgcttggtt
    10681 atggactgat tggttcacta tgggtagatg tgattcaaaa agcatttgtt ccaagagctg
    10741 atgatgggca tgaagtgtct ctggctgatg gaaggcgtgt gcacgtagcc atttcgtctt
    10801 tagagagttt acccggtata ttgattactt tgtctgacat tactgcaagc agggattatg
    10861 aagaagccaa agaaaatgaa aagcgattgg tttctattgg tacaatgacc gcacaattag
    10921 ctcatcagat tagaactcct ttgtcttctg ccatattgta taccgagcat ttaaataatc
    10981 tcccaagttt ggatcatcgc attaaaaact ggattcacag attgcaagaa tgtcatgcca
    11041 gtatagagca acaaattcag gatttattat tatttgccag aggaacctcc atagagccaa
    11101 agtatatcga tatggaattt tggtgctctc aattaatgca aagagctcag ccatacatcg
    11161 agtcttatgc agctgtatta catgttaata accatcttat tgctcgtgaa tcctccatac
    11221 atggagagtc tttgattgga gcaattttaa atctgataat caacgcgctg caatctgagg
    11281 caacccaaat tgatttaaca cttgcatcca tggatgattc aggtatacaa atatcagtag
    11341 aggataatgg aaacggaatg tctgaagaag tcagggcaca agcattttcg ccattctata
    11401 caacaaaagc acaaggtacc ggccttggat tggcggtggt ttttgctgta gtaaaagcgc
    11461 atggcggtcg agtgactctt gagtcgactg aagggatagg aacacaggta actatttatt
    11521 taccttgcct gaacagaaac gtagggtaac tttatcaaac aaaggatttt ctatgagtca
    11581 tgttttaata gttgaagatg attcagtatt aagagaagcg ttggctgaga ctatgacgat
    11641 ctcaggccat acctatatca ctgctaaaga cggtaaagag gcattagcac ttcttgaatt
    11701 acataatcca agtgtcgtac tcactgacat aagaatggat aaattagatg gaaatcagtt
    11761 attaaatgaa atacaaaaaa agaggcctgg tttgccggtt attctaatga cagcttatgg
    11821 ttctgtagag gatgcggtta cagcgattcg caatggcgct gttgattatt tgcaaaagcc
    11881 cttcagcgcg caagtcttaa ttgaaaaaat aaagcagtat ataaaaactg atctgagtga
    11941 agaggataat gatcctattg cacgggatcc taaaagccaa gccttactga caatggcttt
    12001 gagagttgca caatccgatg tgggagtaat gatcagtgga gaaagcggta ctggaaagga
    12061 agtgctcgca cattttatcc atgatcattc ttatcgaagc aaacaacctt ttattgcaat
    12121 taattgtgct gcaattcctg aacaaatgct ggaagcaacc ctgtttggtt atgaaaaagg
    12181 tgcttttacc ggagcataca aatcaacccc tggcaagttt gagcaagccc agggtggaac
    12241 tttactttta gatgaagtga gtgaaatgtc cttaggtttg caggcaaaat tacttagagt
    12301 tttacaggaa aaagaagtag aaaggattgg ttcaaacaaa ataataaacc ttgatgtgcg
    12361 cattattgcg acgacaaaca ggcaattaaa ggaagaggtt ctggcagggc gttttagaga
    12421 ggacctttac tatcgattga atgtttttcc tctgcaatgg cacccactaa gagagagaac
    12481 aagtgatatt attccattag cgaattattt aatccgtaaa cattgtcagc ataaacaacc
    12541 aatagttcca gtcattagcc cggatgcaca aaaggcaatg ttgtcctatt catggccagg
    12601 gaatgcgaga gagctggata atgttataca aagagctctg gtgttacaaa ctcaaggtat
    12661 catcgaagaa gagcatttgc agattacgaa ttcaactacc atgccttcgg aagcgaagac
    12721 aaagggtacg agtaaaaatt tacagataca tgaatatgaa ttaatagaaa aaacattatt
    12781 agaaaatgaa ggaaacagac aaaaagtcgc tgcgttattg ggggttagcg aaaggacttt
    12841 acgctataaa ctcgcaaaaa tgcgtgaaga aggatatgtc gtttaacagg ggctgagaaa
    12901 atgagtgata ttaatacagt aaatttaatt aatcaaatga aaactctggc tgcccaggct
    12961 gaggggataa aactggattc tggtttaaat cacacttctt tcagttcggt ttttcaacag
    13021 gcattgaatc aagtcaatga attatcacaa acagcagata gtttaaagac ccgctttgaa
    13081 ataggtgatc ctaatgtgtc cttgggagaa gtaatgatag caagtcaaaa atccaattta
    13141 gggtttgagg caacattaag agttagaaac aaattggtac aagcatacca ggatattatg
    13201 aacatgccag tttagtacaa tgtttgggta gtagaacttt tcaacagcag ataaacatga
    13261 tcaaactcaa taacaggttt ttatggggtt tttgaatacc ataatgagtt tgcaaatgtt
    13321 ctcagtaaag ttttaagctc atagtgaagt attctggaaa aaaggataaa aattacagga
    13381 gtaaatcatg gcattggctg ataatgcttc agtagcagct acaaaatttg caagcttatc
    13441 tataccacgc caaattggag tgttgtttgg attggcggcc agtattgcaa taggtgtggc
    13501 agtggttcta tggtctcgtg atccaagcta tgttccttta tatagtcagc tcaacgcgca
    13561 ggactcatca gagattatga ctattttaga gcgcaatagt attgatttca aaattgacaa
    13621 gaataatggc ttgttgatgg tgcctgctga tgagctgcaa agcgcgcgtt tgaaattagc
    13681 cgcacaagga ttgcctcgcg atactgccat aggtcatgat cttttaggta gcagtggtgg
    13741 tgtgtttaat accagccaat ttatggaaaa cgcccgatat aagcaagctc ttgaggctga
    13801 attagcacgt acgatcagta aatttaataa tattaaatcg gccagagtac acttggcaat
    13861 tccaagagag tcagcctttg taagagactc acaagagcct agtgcttctg tatttattga
    13921 tgtgtattca ggatttgaaa taaagaaaca taccattgca gcaattgtca atttagtagc
    13981 atcaagtatt ccgaatttaa gcgcaggaag agtaacagta gttgatcaag acggacaatt
    14041 gctaaatgag ggtagcggat ataacctctt ttcggagacc gagcgttttc ttgattacag
    14101 acaaaacctg gaacatcaat acgctcaaaa aattcaggat atcttaaccc caatattggg
    14161 atacggccgt gtcagggcca aagtatctgc agatattgac tttaccagct atgaacaaac
    14221 tcaagaattg ttcaatccgg agctctccgc tttacgcagc gaacaaacta tgcaagagag
    14281 tcgtaatgcg tcaaatgaag caagcggggt tcctgggaca ttatcaaata ctcctcaacc
    14341 aaatagttct cttgggcaaa aaaataatcc tcaacaaaat gcaacgccaa ataacttacc
    14401 tcaacaaaca tcgtcagatc aaatgcaggc aaaggatgtt cgcatgcaaa ccactaaaaa
    14461 tttcgaattg gacaaaacaa taagtcatac taaaaatcag ccaggaacaa taaaacgctt
    14521 gtcggttgcg gtattggtag ataatcgacc tgtaatgaat gataaaacca agaaactgga
    14581 gaagaaaccg ctaactaaag aagaaattga acaaataaag ttattggttt ctgatgccat
    14641 aggattgaat gttaagaggg gagatagctt gaatgtgatc aacagcagtt ttgtaaagcc
    14701 agatcctata gaagcaattc cagaagaaca tttctggcaa aaagacagtt tttggtctgt
    14761 gattaagcag attggcgggg ctctttttat attggcaatc ctatttggtg tattaagacc
    14821 tatgttaaaa acattggcaa gtaataaaga catagatcaa gccactgata atcaggatac
    14881 aggagacttt acattagatg gaaaaatatc attcagagat gttcaggatt atgaaactca
    14941 gctgagtttg cttcgtcaag tggttgataa agagcccaaa cgcgtcgcac aagtagtgaa
    15001 atcatgggtt gatcgggggt aattatggac ggaatagagc gagcagctat tttattgtta
    15061 agcatgggag aaaaaaatgc ttcagaagta ttaaagcatt tagagcccag gcaagtacaa
    15121 aaagttggaa tggctatgtc aacgatgaat agcgtcagta aggcgaaaat gcagaatgtt
    15181 ctgattgact ttatcagcgc aatcgaagat caaaccagct taaccgttga tacagagcat
    15241 tacctcagaa ccgttctcat tgaggcattg ggtgaagaaa aggcaattcc atttattgat
    15301 agaatattgg tatcaaataa agacagtggt ttgaataagc tgaaatggtt ggatggacgt
    15361 ctgatagctg aagtgattcg taatgaacat ccccaaatca ttgccactat attaattcat
    15421 ctggacagtg agcagtccgc tgaagtggtg agttacttta gcgcagaaaa aagagcagaa
    15481 gtgttattac gaatgtgtaa tattgataca gtaaaacctg aagcaatttc tgaacttgga
    15541 catgtgattg aaaagcagct aagtggtcaa accacaggta aatcatcttc tgtcggcgga
    15601 attaaaaccg tagcagatgt gattaatttt ttggatggtg ctatggaaga agaggtactg
    15661 gagaggatta aggattggga tgaggagtta agtgaaaaga taagagataa gatgtttgtt
    15721 tttgaaaaca tcaaagacat ggatgataga agtgtacaaa ctttattgcg agaggtgaca
    15781 tctgatcaat taaaattagc attgaagggt actactgaaa caacgaaaga aaaaatctat
    15841 tcgaatatgt ctaagcgagc cgctgatctc ctcagagatg atatcgaggt gcagggacct
    15901 gtaagagtca gtgatgtgga gcgtgcccag cgagatattt tagccatagc aagaaatctg
    15961 gctgaagatg gcaagattag cctgggagca aaggggggag atgaattaat ctagttcctt
    16021 ttcccaggat gagttttaga ctttttatga aaatagtaaa aatcattaag caagagtccg
    16081 tattggaatt gtattttatt tgtcctaagt ggtaattttt ggtatgattt gagcccggga
    16141 taaggttgga taactatgtc aaatgaattt gagccgtata ataaagaggt aaaaaaaatt
    16201 gaatttacca cttggaagta tcaggtttcc aacgatgctg aaaattcgcc gtctgatgaa
    16261 gaagagttga ttaatgaatt ggaggtagtt aagcaggaag cttttcaaaa aggttatgca
    16321 gatggatttc aacaagcaca agaagaaatt aatgcaatta aagcggagct ctccaaatgg
    16381 ctggatttaa ttcaaaagcc cgttcaaatt ttagatgagc aattgacgca ggaaatcatt
    16441 caaaccatca tttggttaag tcaacactgt gttgctgttg
//
LOCUS       DAXLDT010000065        16406 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXLDT010000065 DAXLDT010000000
VERSION     DAXLDT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.065"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..807)
                     /locus_tag="ACF2SP_001676"
     CDS             complement(<1..807)
                     /locus_tag="ACF2SP_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFD2359464.1"
                     /translation="MAKILITGATGFIGRSLVPALLSEGHDVRCAVLQLDSTLQAEQI
                     VINNLEVHTDWTDALRNVEIVIHLAARVHIMKEYATSSLDEYCKINSIATKNFVEQAV
                     QNNVKRFIFLSTIKVHGEFSQNNLPFSEDCRTQPEDPYAKSKLYAEQFIQEICQNARM
                     EFVILRPPLVYGPYVKANFLRILQLVDKKWPLPFGSIYNKRTFIYIDNLVSAISAVVS
                     EPSAANQVYLVADDCSWSLTQLVQTLSRKMNTKLFLIPIPVQILIFLFKLC"
     gene            complement(828..1703)
                     /gene="rfbA"
                     /locus_tag="ACF2SP_001677"
     CDS             complement(828..1703)
                     /gene="rfbA"
                     /locus_tag="ACF2SP_001677"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFD2359465.1"
                     /translation="MKGIILAGGFGTRLYPLTKSISKHMIPVYDKPMIYYAISTLMLA
                     NIREILIISTEEHLPLYQNLLSDGSQWGISFAYLVQNEPRGIAEAFILGEKFIGNDSV
                     CLVLGDNIHQGRGFSELLQNAKSKLNGATVFAYYVDKPQAYGVVEFNEKQQVLSIVEK
                     PAQPKSNYAVTGLYFYDNQVIEIAKSLKPSGRGELEITDINQFYLYENKLDVQILGRG
                     FVWLDMGTPETLLSASNYICTIEQRQGLKIGCPEEIAWRMKFISDDELLCRARELNKS
                     SYGQYLAELLNHKRY"
     gene            complement(1858..3342)
                     /gene="pgi"
                     /locus_tag="ACF2SP_001678"
     CDS             complement(1858..3342)
                     /gene="pgi"
                     /locus_tag="ACF2SP_001678"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFD2359466.1"
                     /translation="MKSHTELLSWNLLQKEADRVRLNSDSLTCVVPDSNNYESSKQIN
                     CIEYDYSRQRVNRTIIDLLIDLANEVKLQEKIDNLINGKKINISENRPALHTALRDLG
                     NKSIMIDGLDIMSAVINTREKIKVISNQIREKKWLGHSGLPITDIVNIGIGGSDLGPR
                     VCINALSNYISKEFNYHFISDVDPASFNDVIAKINPQTTLFIVSSKSFTTKETLLNAR
                     KAFALYEDTASIDQHFIAVTAHPERAYQMGIKTVLPIWDWVGGRFSFCSAVNLITAIA
                     IGYEQFVELLAGAHDVDTHVQFTDFKNNIPVLMALIGIWNNNFLNIHNLLILTYSKKL
                     EYFVPYVQQLDMESNGKSIDVNGSMVDYATGPIVWGGLGNQAQHSYFQLLCQGTHRCV
                     GDFITLKTNDEHEINSMCHYKMKVLSEGIQTIENPYGYIPGNMPMNHLILSDCSPYTL
                     GALVALYEHKIFVQSVIWNINPFDQPGIESAKSAHREITLSSES"
     gene            complement(3396..4475)
                     /gene="rfbB"
                     /locus_tag="ACF2SP_001679"
     CDS             complement(3396..4475)
                     /gene="rfbB"
                     /locus_tag="ACF2SP_001679"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFD2359467.1"
                     /translation="MICQMLSTILQKSKNLTMKNQMNNLLVTGAAGFIGSNFVKFMND
                     KYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILDKSLVLSLLREYEIDTLVH
                     FAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVY
                     GSLEREEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQH
                     KEKLIPKVVYACVNQLPITVYGNGSNIRDWLYVMDHCEAIDTIIQKGALGEVYNIGGN
                     NELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNSKIQKELGWVP
                     QGDFVHKLSNTVHHYLTRYEREINI"
     gene            complement(4435..5319)
                     /gene="rfbD"
                     /locus_tag="ACF2SP_001680"
     CDS             complement(4435..5319)
                     /gene="rfbD"
                     /locus_tag="ACF2SP_001680"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFD2359468.1"
                     /translation="MKILVTGANGQVGTEIIKRFSSSEHEVYACTRDILDCSKLERVH
                     DVLSEIKPDLIINAAAYTAVDKAEDEPDLAHIVNAEFVCRLVNYCTLKNVPLIHLSTD
                     YVFDGEKEGAYHETDIPHPVSTYGRTKWEGEQAILSQLKKYIILRVSWVFGEQGKNFV
                     KTILNLASSRKELNIVSDQSGRPTSARDIARVLFEIVQKIRHSSFDYWGLYHYAGQGV
                     TNWHEFANVFLNMAKEKQLLLTLTDLNPIKSQEYPTRATRPKNSVLDTTKIETILGIK
                     SCSWKNDLPDVIDHFTKK"
     gene            complement(5320..5883)
                     /gene="rfbC"
                     /locus_tag="ACF2SP_001681"
     CDS             complement(5320..5883)
                     /gene="rfbC"
                     /locus_tag="ACF2SP_001681"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFD2359469.1"
                     /translation="MNIINTQIDELKIIEPKIYGDERGFFYESFQAKRYEELLGITDR
                     FVQDNFSRSQKGVLRGLHYQSQQTQGKLVSVLAGEVFDVAVDIRLGSPTFGQWVGVIL
                     SGENKRQFWIPKGFAHGFYVLSAMADFAYKCTDYYHPESEFSIHYLDPQLAIDWPLGE
                     QVQLSPKDAAAKLLNLIDAELLPRYQA"
     gene            complement(5973..7475)
                     /locus_tag="ACF2SP_001682"
     CDS             complement(5973..7475)
                     /locus_tag="ACF2SP_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFD2359470.1"
                     /translation="MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVT
                     DGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNR
                     PIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
                     KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDT
                     WNMSPVALERALRESKAKNQMPKAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAES
                     LGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQRARFLSTQAREPMP
                     HYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEI
                     TQGYSTRWLSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPH
                     DEQFSVSDYLFDHGICLPSGSNLTPRDIDRVIHCLNDIFIKYKQNNFEVA"
     gene            complement(7486..8094)
                     /locus_tag="ACF2SP_001683"
     CDS             complement(7486..8094)
                     /locus_tag="ACF2SP_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFD2359471.1"
                     /translation="MHTNLKIIGCGGHCKVVIDALSLTQHSLQVSLCDSNKSFLGQEV
                     CGVLVDSTMDSLADYIGYVHVSIGNNQARKNVIDLMNSNANLFTIIHPAAIIAKSARL
                     GAGSFIAAQAILGPECQVGEGCIINHSAVVDHEVIVGSCSHIAPNSTLGGRVKIGERV
                     LIGAGAVVLPGVTIGDGAIIGAGSVVVKDVKENAVVKGVPAV"
     gene            complement(8084..9217)
                     /gene="neuC"
                     /locus_tag="ACF2SP_001684"
     CDS             complement(8084..9217)
                     /gene="neuC"
                     /locus_tag="ACF2SP_001684"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFD2359472.1"
                     /translation="MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDA
                     LYGNTVNEIKADQFSICGIIPVDLANAQHSSMAKAIGHELLGFTEVFESETPDVVLLL
                     GDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
                     IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFDPDKKICLLIYHPVVQEVDS
                     IKIQFQSVIQAALATNLQIICLEPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFI
                     DCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLR
                     EALNKPKIKYSNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY"
     gene            complement(9217..10239)
                     /gene="legI"
                     /locus_tag="ACF2SP_001685"
     CDS             complement(9217..10239)
                     /gene="legI"
                     /locus_tag="ACF2SP_001685"
                     /EC_number="2.5.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetyllegionaminate synthase"
                     /protein_id="HFD2359473.1"
                     /translation="MTCFIIAEAGVNHNGDLQLAKELVYAAKESGADAVKFQTFKADT
                     LVNKTVEKAEYQKNNAPESSTQYEMLKALELSEEDHYLLSELANSLGIEFMSTGFDEQ
                     SIDFLISLGVKRLKIPSGEITNVPYLQHCASKKLPLIISTGMCDLQEVRVAIDTVKPY
                     YGNSLSDYLVLLHCTSNYPASYQDVNLKAMQTLADEFQLPVGYSDHTLGILVPTLAVG
                     MGACVIEKHFTMDKSLPGPDHLASMDPEEMKNLVQSIRDAETVLGSGEKKPSDNELPI
                     RALVRRSITLRRDLVKGAQISKEDLILLRPGTGIAPSEISNIVGSRLSMNLSAGTTLL
                     WEHIEA"
     gene            complement(10236..10934)
                     /gene="neuA"
                     /locus_tag="ACF2SP_001686"
     CDS             complement(10236..10934)
                     /gene="neuA"
                     /locus_tag="ACF2SP_001686"
                     /EC_number="2.7.7.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N,N'-diacetyllegionaminic acid synthase"
                     /protein_id="HFD2359474.1"
                     /translation="MRILAVIPARAGSKRLPGKNTRLLAGKPLIAHTIVAALQSSCCE
                     EIVVSTDSKQIADVAVQYGASVPWLRSEDLATDTSDVIHTVIDLLFKFQQMDVFFDSV
                     LLLQPTSPFRKPETIRHAVEIHKVTGKSVVSVSPISLKPSWCRSIDSQGNLVKPELFQ
                     DLEIYCNENPIYKLNGSIYIATAKQIIENKSFYSEPTKPLLLNSISESIDIDTPIDWA
                     LTEKLMELNQEALV"
     gene            complement(10947..11588)
                     /gene="hisH"
                     /locus_tag="ACF2SP_001687"
     CDS             complement(10947..11588)
                     /gene="hisH"
                     /locus_tag="ACF2SP_001687"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFD2359475.1"
                     /translation="MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLV
                     LPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDL
                     IAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
                     AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI"
     gene            complement(11585..12346)
                     /gene="wbuZ"
                     /locus_tag="ACF2SP_001688"
     CDS             complement(11585..12346)
                     /gene="wbuZ"
                     /locus_tag="ACF2SP_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl amidation-associated protein WbuZ"
                     /protein_id="HFD2359476.1"
                     /translation="MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGA
                     DELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAI
                     NTAATKNPTLISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
                     GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCES
                     KADAVAMADVLHYKRIGLPEIRKYALDNGIHVRVL"
     gene            complement(12346..13701)
                     /locus_tag="ACF2SP_001689"
     CDS             complement(12346..13701)
                     /locus_tag="ACF2SP_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HFD2359477.1"
                     /translation="MSKIYAPKSIDLSSFQLPDEKLQTKYGLPHKVEFCKSCVISNQR
                     PNSAVEYEHKKESKKHTIHFDDEGICDACRVAERKKSTINWEERDRQLRELCDRFRSK
                     DGSYDCVVPGSGGKDSFYAAHILKYKYGMNPLTVTWAPHMYTPWGWRNFQSWIHAGFD
                     NHLFTPNGRVHRLLTRLAVENLFHPFQPFMIGQKAYAPKMALLHKIKLVVYGENEAEY
                     GNPIGDTESAKRDWKYFTADDKSKIFLGGTSVQELKSDFGLNDNDLDAYLPADPQQIE
                     EQQVEVHYLGYYLKWHPQSCYYYSVEHGGFEASPERTPGTYSKYNSIDDKIDDFHYYT
                     TLTKFGIGRATYDASQEIRSGDITREEGVALVKRFDQEFPERFAEEIFKYLSINLKEF
                     PIASQMFEQPIMDRAYFMALADTFRSPHLWKKDGEQWKLRHQVTNLEKTKAEYLDLET
                     V"
     gene            13888..14706
                     /locus_tag="ACF2SP_001690"
     CDS             13888..14706
                     /locus_tag="ACF2SP_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFD2359478.1"
                     /translation="MSLKAMYNQIAENYATANRFGSISESHHVAIEQMRKFHLGLKPH
                     YKILDLGVGNGSFLQKLHHLMPMANFTGIDVSSEMLKRASEALPLTTIEGSAAEANKF
                     LPAHSQDLVLAHFINAYIPINVLFDEAKYLTRANGHFSLITTTYDSFPVAQQQLANFI
                     AQDTILSRVVGHYYKSIVKNTTVAANLDELLLAFKQHQFNILDHQRIEIPITLNNIDE
                     LALFGIEGTWFLNTLSIRMLPKYFLIQRLKRLFSKIFTFPYHDTHIIDVVLARK"
     gene            14745..16253
                     /locus_tag="ACF2SP_001691"
     CDS             14745..16253
                     /locus_tag="ACF2SP_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFD2359479.1"
                     /translation="MKLISQAVKNYLPELSLRQKQTNNIIFITFWSQFSVYALNTVLV
                     LFLTRPLIAQGLGYSQAKAYAFIGVTQATGYLMPILGGYMADNIVGVRRSILLGSIML
                     ACAYLLVMLSGYTISSLGDQLFIIAFAFIPATNSLLMGTASSMVSHIYSDDAIKAKSA
                     MTYYYMAINVGALLATMIAPVLLESRYGPLSVLTLTFIGKSIAALNFAKRYSIYDSVI
                     WGMDKSKFSNQGLLRLVAYIAAIYSLTLYAYTHVYIASTLIGAGCAIGILWFLIKTIM
                     LKGETRSKQMIAVLLIIEAIVFFIIYNQMNSTLVLFAENNSDLSFLWFKISPAQYQML
                     NPLLILLIGSQLPRFYRFFSRFTIPYQFAAGTILSGIALLVMAFAAQNAINGLVNGNY
                     IALTYILISIAELWVSAIGLSMIGLYCDARAIAFAMGVWYLASSMSNAISGRIAGWVA
                     IPENINSALESLPYYKNYYLIMGISALGLGILMYFVAYYLHKIMKRRGIELA"
BASE COUNT         4930 a         3464 c         2733 g         5279 t
ORIGIN      
        1 acataattta aatagaaaaa ttaaaatttg aactggaatc gggattaaaa ataattttgt
       61 attcatcttg cgcgataagg tttgcactag ttgtgttaaa gaccatgaac aatcatctgc
      121 taccaaataa acttgattag cagcactagg ctcgctcaca accgcgctta ttgctgaaac
      181 caaattatca atatatataa aagttcgttt attataaata ctaccaaaag gcaaaggcca
      241 ttttttatca acaagttgta atatcctgag aaaattagcc ttcacataag gtccatagac
      301 caacggtggt cttaatataa caaactccat ccgggcattc tggcaaattt cttgtataaa
      361 ttgttcagca tacaacttac tttttgcata gggatcttca ggttgagtcc tacaatcttc
      421 agaaaatggc agattatttt gtgaaaattc gccatgtact tttatggtac taagaaatat
      481 aaatcgcttt acattatttt gaactgcctg ctcaacaaaa ttttttgtag caatactatt
      541 aattttgcaa tattcgtcta aagaggatgt tgcatattct ttcattatgt gaactctggc
      601 agctaaatga atcactattt ccacattccg caaagcatca gtccaatcag tgtgaacctc
      661 aagattgttt attactattt gttctgcttg taatgtacta tccagctgca aaacagcaca
      721 acgcacgtca tgtccttcag aaagcaaggc aggaactaaa cttcgtccta taaatcctgt
      781 cgcgccggta attaatattt ttgccactac tttataacct gaaaaaatta atatcgttta
      841 tgattaagca attctgcaag atattgaccg taactacttt tattaagctc tctagccctg
      901 cacagtaact catcatcact aataaacttc atacgccacg cgatttcttc agggcatcca
      961 atttttaaac cttgccgctg ttcgatagta cagatatagt tacttgctga taacagggtc
     1021 tctggtgtac ccatatccag ccagacaaaa cctcttccca aaatctggac atccagtttg
     1081 ttttcatata aataaaactg attgatgtca gttatttcca gctcccccct cccagagggt
     1141 tttaaactct tagcaatttc tataacttga ttatcataaa aatataatcc ggttactgcg
     1201 tagttggatt tgggctgcgc tggtttttcc actatggata gtacttgttg tttttcattg
     1261 aactccacca caccataggc ctgaggttta tcgacataat aagcaaatac tgttgcaccg
     1321 tttaattttg attttgcatt ttgtaataat tcagaaaaac ctctgccctg gtggatatta
     1381 tcccccaaaa ccaggcaaac agaatcattg ccaataaatt tttcacctaa aatgaatgct
     1441 tccgcgattc ctcttggttc attttgcaca agataggcaa atgagatgcc ccactgagag
     1501 ccatcactca ataaattttg atacagcgga agatgctcct ccgttgaaat aatcagtatt
     1561 tccctgatat tagccaacat taatgtagag atggcataat aaatcattgg tttatcataa
     1621 accggaatca tatgcttact aatggatttt gtcagaggat acagtcttgt accaaaacca
     1681 cctgccagga taataccttt cattatatcg agcctactct ctatttttct ttgaacaaat
     1741 aacagcctta aatattatca agtttattga agtaatacat taaaatacgt tgataatgtc
     1801 aataaaatat aaagtttagt ttaataatta aactaattta tagcattgag cgccccttta
     1861 tgattcgctt gataatgtga tttctctgtg agcactcttg gcgctttcaa taccgggctg
     1921 atcgaatggg ttaatattcc atatcacact ttgtacaaaa atcttatgtt catagagtgc
     1981 caccagagcc cctaatgtat aaggagaaca atctgacaaa atgagatgat tcattggcat
     2041 attgccagga atatacccat aaggattttc aattgtttga attccctcac ttaaaacttt
     2101 cattttataa tgacacatgg aattaatctc atgctcatca ttagttttca aagtaataaa
     2161 atcaccaaca catcgatgcg taccctgaca taataattga aaatagctat gctgtgcctg
     2221 atttcctaat ccaccccaaa cgataggtcc agtggcgtaa tctaccatac ttccattcac
     2281 atcaatggac ttcccattac tctccatatc cagctgttgt acataaggaa caaagtactc
     2341 cagttttttt gaataggtaa gaataagtaa attatgaata ttcaaaaaat tgttattcca
     2401 tataccgatt aaagccatta gcacaggaat attattttta aaatcagtaa attggacatg
     2461 agtatcgaca tcatgggcac cagccagcaa ttcaacaaat tgttcataac caattgcaat
     2521 cgcagtaatt aaattaactg cggaacaaaa agaaaatcga ccaccaaccc aatcccaaat
     2581 cggtaaaaca gttttaatac ccatttgata agctctttcc ggatgtgcgg ttacagctat
     2641 aaaatgttga tcgatagagg ccgtatcctc atacaatgca aaagcttttc tggcatttaa
     2701 tagagtttct tttgtagtaa atgatttgga agaaacaata aataaggtgg tttgcggatt
     2761 aatttttgca ataacatcgt taaaactggc cggatcaaca tctgagataa aatgataatt
     2821 aaattctttg gaaatataat tagacagggc attgatgcat acacgaggac ctaaatccga
     2881 gccgccaata cctatgttta caatatcagt tattggtaga cctgaatggc ccagccactt
     2941 cttttctctg atctgattgg atataacttt aattttttca cgagtattta taactgcgga
     3001 cataatatcc agtccatcaa tcattatcga tttattacct aagtccctaa gtgcagtatg
     3061 tagtgcaggc ctgttttcgc tgatattgat ttttttacca ttgattaagt tatcaatttt
     3121 ttcttgtaat ttgacttcat tagccaaatc gataagcaaa tcaatgatgg ttctgtttac
     3181 tctctgcctg gaatagtcgt actcaataca attgatctgt ttactggatt cataattgtt
     3241 ggaatccgga acaacgcatg ttagagaatc tgaatttaga cgcactctat ccgcttcttt
     3301 ttgaagcaaa ttccatgaca ataattctgt gtgagacttc atgctatttc taatcatcaa
     3361 aataaagtct atgttacctg attattttca atttattaaa tattaatttc gcgctcatac
     3421 cgtgttaagt aatgatgaac tgtatttgat aatttatgaa caaaatcacc ttgtggaacc
     3481 cagcccaatt ccttttgaat tttactattg tctatggcgt atcgtttgtc atgacccttt
     3541 ctatcttcaa caaaagtaat caatgaatga tatggttttt ccataggttt taaatcatcc
     3601 atcatctgac aaatcatttt tatcaaactt aaattatcca gttcgttatt accacctata
     3661 ttataaactt cccctaatgc tcctttttga ataatggtat caattgcttc gcaatgatcc
     3721 atgacataca accaatcgcg aatatttgat ccgttgccat aaacggtgat gggcagctga
     3781 ttgacacaag catatactac tttaggaatc agcttctctt tatgttgatt aggcccgtaa
     3841 ttattcgaac aattcgaagt ggtaacaggt aatccatagg tatgataata agctctgact
     3901 atatgatcag aacttgcctt ggacgctgaa taaggggaat tgggttgata tgaatttttt
     3961 tcagtgaacg ccggctcttc tctttctaat gaaccataca cttcatcagt ggatacatga
     4021 tgaaaccgac actttgattt atcccattgc ctttcattca accaataaat ccttgccgcc
     4081 tctaacagag taaaagttcc aattacattg gtttcaagaa atatttgtgg attatcaatg
     4141 gaattatcca catgggattc ggctgcaaaa tggacaagcg tatctatttc atattccctt
     4201 agtaaggata acaccagact tttgtccaat atgtctcctt gcacaaaaag gtgattttta
     4261 cattctgcca tttcactcaa attggcttta tttcctgcat aggttaattt atccaaactg
     4321 attattttga tctccgggta tttatcgttc ataaatttaa caaaattact tcctataaag
     4381 cccgcagctc cagttaccaa aaggttgttc atctgatttt tcatagtgag attcttactt
     4441 ttttgtaaaa tggtcgataa catctggcaa atcatttttc caactacagc tttttatacc
     4501 caatattgtt tctatctttg ttgtatccag taccgaattt ttaggccttg ttgccctagt
     4561 aggatattct tgtgacttaa ttggatttaa atcggttaaa gttaagagaa gctgtttctc
     4621 ttttgccata ttaagaaata cgtttgcaaa ttcatgccaa ttggtaacac cctgccctgc
     4681 ataatggtaa agcccccaat agtcaaagga agaatgtctg attttctgta ctatttcaaa
     4741 tagtactctt gctatatcgc gagctgaagt aggccttccc gattgatctg aaacaatatt
     4801 caattctttt ctgctggaag ccaaatttaa aatcgttttt acaaaatttt tcccttgttc
     4861 accaaatacc cagctaactc gcaaaataat atacttttta agttgggata aaatagcctg
     4921 ttctccttcc catttcgtgc gcccataggt acttactgga tgcggaatat cggtttcatg
     4981 ataggctccc tccttttctc catcaaatac ataatcagtt gaaagatgga tcaaggggac
     5041 gtttttcaag gtacaataat tcaccaggcg acagacaaat tcggcgttca caatatgagc
     5101 caaatcaggt tcatcctctg ccttatctac agccgtatag gctgctgcat taatgataag
     5161 atccggcttg atttccgata acacatcatg aacccgttcg agtttggaac aatctagaat
     5221 atctctggta caagcatata cttcatgctc agaggaagaa aatcgcttga taatctcggt
     5281 accaacttgc ccattagcac ctgtaaccaa tattttcatt taggcttgat atctcggtaa
     5341 aagttcagca tctattaaat ttaaaagctt ggctgcagca tcttttggag acaattgaac
     5401 ctgctctcct aatggccaat caattgctaa ttgcggatca agataatgaa tggaaaactc
     5461 actttcagga tgataatagt cagtacattt ataagcaaaa tcagccatag cactcaaaac
     5521 ataaaaccca tgggcaaatc ctttaggaat ccaaaattgc cttttgtttt ctccggaaag
     5581 aatcactcca acccattgcc caaaagtagg ggagcctaac ctaatatcca cggcaacatc
     5641 aaacacttca ccagctaaaa ctgaaaccag ttttccctga gtttgttgac tttgataatg
     5701 caaacctctt agtacacctt tttgtgatct tgaaaaatta tcctgtacaa aacgatcagt
     5761 tattcctaat aactcttcat atcgtttagc ttgaaaagat tcataaaaaa accccctttc
     5821 atcaccataa atctttggtt cgataatttt aagttcatcg atctgagtat ttattatatt
     5881 catatcgtta ctaaagctct taaaagagaa ttataattcc ttaatattta aatcttttac
     5941 tacctgtatc cagttttcac tagaacaaat aattaagcta cctcaaaatt gttttgttta
     6001 tatttaataa aaatatcatt caaacaatga atgactctat caatgtctct gggggttaaa
     6061 tttgacccgg aaggaagaca aataccatga tcaaacaagt aatctgaaac gctgaattgt
     6121 tcatcatgag gatagtattt agagcctgca aataaaggct gtctatgcat aggcttccat
     6181 gttcttcttg cttcaatgtt aaatggcttt aattgctcaa tgacttcttc cggttttatc
     6241 ttgtttggcg ttggtttaat aaccaatgtc gataaccaac gagtactgta tccttgggtt
     6301 atctctggca tcatgtcaat aaatgggtaa gattccaagg cttgcttata tcgttcaaaa
     6361 atagctcgtc ttgccttaac ccgtttttcc aatactttca gctgacctct gcctatacca
     6421 gcaagcacat tactcattct gtaattatac ccaaccacag tatgttcata atggggcatt
     6481 ggttcgcgag cctgagttga taaaaatctt gctctttgta tgagactctc atcttcagac
     6541 accaacatgc cgccaccaga cgtggtaatg attttgttgc cattaaatga aaacacgcct
     6601 aacttgccga aagtcccgct gtacttgtta ttataggtgg ctcccaaaga ctccgccgca
     6661 tcttctataa ttggcacatt gtattcattg cataattgac ataaagcgtc ataattagca
     6721 ctttgtccat ataaattaac aataatcaca gcttttggca tttgattttt tgctttactt
     6781 tctcttaatg cccgctcaag agcaactgga gacatattcc aggtgtccaa atcagaatca
     6841 ataaataccg gagtcgcacc ttggtataag ataggattca ctgtggctac aaaagtaaag
     6901 ctggatgcga acacaatatc tcctggtttc acatcgagta atacgagtgc cagatgaatt
     6961 gcagctgttc ctgagcttaa agccacagct gattttatat taatgtattc cgccacttct
     7021 ttttcaaaag aatcaacatt ggggcctaaa ggggctaccc agttggtggc aaaagcttcc
     7081 tgtacgtatt gctgctcatg ctcaccgata tgaggcgagg aaagcagaat attggaagag
     7141 agttctctcc ggtggatcaa gcgtatgggg cgattactct catcgataac gggaatatga
     7201 tctaattcat attcctgcat caaatgataa gcatcctgaa tcgtagcaga gacaggtaaa
     7261 gcttttgatg aatgctctga taattcctct aaagtagaat ctaatgtaaa tccttttaat
     7321 agcaatcttc ggatgtcacc atctgtaact gtccttatta aatgttcatc actatctacc
     7381 agaaataaca caccttgagc tgtagcatcc agcttgctca aagcagtttt cagattttct
     7441 tgtttttgta tatataactc tttgatagta atcattttat tccttttaaa ctgcgggtac
     7501 gcctttcaca acagcatttt cttttacatc cttcacaacg actgagccag ctccaattat
     7561 tgctccatca cctatagtca caccaggcaa aacaactgct ccagctccaa tcagcactct
     7621 ctcaccaatc ttgactcgcc cacctaaggt gctattaggc gcaatatgtg aacacgagcc
     7681 aactatcact tcatgatcca caacggcact gtgattgatg atacatccct cacccacctg
     7741 acattcaggg ccgagtattg cctgtgctgc aataaaggag cctgcaccga gcctcgctga
     7801 tttcgcaata atggcagccg gatgaataat tgtaaacaag tttgcattgg aattcatcaa
     7861 atctataaca ttctttctgg cctgattatt tccaatgcta acgtgcacat aaccaatata
     7921 gtcagctaaa ctgtccatag ttgaatcaac taatactcca cacacctcct gccccagaaa
     7981 actcttattg ctatcacaaa gtgaaacttg taaagagtgc tgagtcagtg ataaagcatc
     8041 aattaccacc ttacagtgtc caccacatcc tattattttc aagttagtat gcattgcatt
     8101 tattcaatat ttgtgagtgc aaagggatag tttttaacaa ttgataacac ctttcactcg
     8161 tttttccatc cccataacag ttagagtatt ttatcttggg tttatttagc gcttctctta
     8221 gaccagtcaa aatagcatca taagtaacat caacatcaat gacattgtcg cttcgttctc
     8281 ttaaattttg cctgcttcca acattaacta cgttcaggtt aaatgaggct gcctctatga
     8341 tgccactact ggaatttccc agcatcacat cagaatttgc aagacaatca ataaattccg
     8401 gacgatgtaa gtgcttgata attctaacat caggatgatc aatatattcc tgaatcactt
     8461 ctcgaattaa atgaccaccc gtatcggaat taggctcaag gcaaataatc tgtaaatttg
     8521 tagcgagtgc tgcctgaatc acgctttgaa attgaatttt aatcgagtca acttcttgaa
     8581 caaccgggtg atagattaat aaacagattt ttttgtcagg atcaaatcca taacgttgat
     8641 taaacacatc acgtgtagac gttttatact gcatgatttc atccaagcct ggagcaccaa
     8701 cctgaaaaat ggtttcttct ttctcaccca ttctaatcaa tcgttgtttg gatgcctctg
     8761 ttgcgacaaa atgataatga gataatttgg aaatcgcatg ccttaccatt tcatcaacgg
     8821 ttccagagcg ctctcctccg tgcagatgta caaccgggat atttaaatgt atcgctgcta
     8881 tggccgcagc aagcatttct cctcgatctc ccagcaataa aacgacatca ggagtttcac
     8941 tttcgaatac ctcggtgaat cccaaaagtt catggccgat agcttttgcc atagaactat
     9001 gctgagcatt ggcaagatca acaggaataa tgccgcatat tgagaactga tctgctttaa
     9061 tttcgttaac tgtatttcca tacaaagcat caagatgcat accagtgaca caaatcgata
     9121 agtcaatgtc ttctgactgg tgtaatcttt ttagtacttc tctcatcagt ccataatcgg
     9181 cacgagtacc tgtaacataa attatttttc tgatcattac gcttcaatat gttcccataa
     9241 caaagtagta cccgcagaca aattcatact taatcgagag cctacaatat ttgatatttc
     9301 agagggcgct atcccagtac caggtcttag caagatcaaa tcttctttac tgatttgtgc
     9361 tcctttcacc aaatcacgtc ttaaagtaat acttcttcta accagagctc ttatcggcag
     9421 ctcattatcc gacggttttt tttcaccact tcccaaaaca gtttcagcgt ctctaatgga
     9481 ttgtactaaa ttcttcattt cctctggatc catagaagcc agatgatctg gaccaggaag
     9541 agatttatcc attgtaaaat gcttttcgat aacacacgcc cccattccta ctgctaatgt
     9601 tggcaccaaa atacctaaag tatgatcaga ataacctact ggcaattgaa attcgtcagc
     9661 taaagtctgc atggctttca gattcacgtc ttgataagat gccggataat tagaagtgca
     9721 atgcaacaaa accaaataat cggataaaga gtttccatag tagggtttta ctgtatctat
     9781 ggctactctt acttcttgta gatcgcacat ccctgttgag atgattaaag gtaacttctt
     9841 actggcacaa tgttgtaaat aaggtacatt agtaatttcg ccagaaggga tttttaatcg
     9901 ctttacccct agggaaataa gaaaatcgat gctttgttca tcaaaaccag ttgacataaa
     9961 ttcaatgccg agtgaattgg ctaattcact tagtaaataa tggtcctctt ctgaaagttc
    10021 cagtgccttg agcatctcat actgagtaga ggattccggg gcattatttt tttggtattc
    10081 cgctttttct actgttttat taaccaaggt gtccgctttg aaagtctgaa actttactgc
    10141 atcagctcct gactctttgg ctgcataaac taattcttta gccagttgaa gatcaccatt
    10201 atggtttact cctgcttcag caataataaa acaagtcata ctagagcctc ttggtttaat
    10261 tccattaatt tttctgttag agcccaatcg attggcgtat cgatgtcgat ggattcacta
    10321 atactattta acaataaagg tttggttggt tcgctataaa aactcttatt ttcaataatt
    10381 tgtttagctg ttgcaatata aattgaccca tttaatttat aaattggatt ctcgttgcaa
    10441 taaatttcca gatcctgaaa aagttctggc ttaaccaaat tgccttggct atcaatgctt
    10501 ctacaccaag aaggctttaa agagatggga ctaactgaaa cgacactttt ccccgttact
    10561 ttatgtattt caacagcatg tcttatggtt tctggcttcc taaatggaga agttggttgt
    10621 aacagcaata cactgtcaaa aaaaacatcc atttgctgaa acttaaacag gaggtcaata
    10681 acagtatgaa tcacatccga agtatccgtg gctaaatctt ccgatcttag ccagggtact
    10741 gaagccccat attgaacggc gacgtctgct atttgtttac tatcggttga aacaacgatt
    10801 tcttcacaac aagacgactg caaggcagca acaatagtat gtgcaattaa tggctttccg
    10861 gcaagtaatc tggtattttt accgggcagc ctctttgagc cagcccttgc cgggattact
    10921 gccaatattc tcatttttaa ttcaccctaa atctgcaaaa actgttgaat gatgcttaaa
    10981 cctacttccc cgcttttttc aggatgaaat tggcatccgt acatattttc atcttttatt
    11041 actgcgctaa tagcctgacc atcatataaa gtatcagcca gacgcttttt aggattacta
    11101 ggcatggcca taaaagaatg aacaaaatat actgaagaat ttaatggtat atttttcagg
    11161 atagtatgac accaatcctc gccttctgag gtggaaacca gttcattcca gcctatatga
    11221 ggtattttat gcaattgacc atcaactcca tgtgaaggaa cgctaacaac ctcgccagct
    11281 attagatcca ggccttcatg ttgtccgaat tcagtacttc ttgagagcat catctgcatg
    11341 ccaagacata ttcccagaaa aggttttcca ctccgcgcaa attgctttat gggttctata
    11401 aaatttaatg tagttaatcc tttcatccca tcttcaaaag cgcctactcc tggcaaaacc
    11461 aaccgatcgg cactcataat ttcctcgggt gtgctcacta aattgacaga agcatcaaaa
    11521 tattgaaaag ctcgagccac acttaataaa ttgcctaccc cataatctac aattgaaact
    11581 gaactcataa gactctcaca tgaatgccat tatctaaagc atactttctg atttcaggta
    11641 aacctattct tttgtaatgc aaaacatccg ccatagcgac tgcatcagcc ttagactcac
    11701 aaaccacttc agttaaatct tccagcttac ccataccccc acttgcaatc accgggatag
    11761 aaaccatgga agacacctgt ttcactaatt ccagatcaaa tcccttacgc gtaccttcct
    11821 gatcaataga ggtcagaaga atttcacctg caccaaattc ttcgccattc ctagcccaat
    11881 caacaacatc cattccggta tgttcacgtc cattgtctgt catgacctcc catcttccat
    11941 tcacagtgcg ttttgcttcg atagataaaa caacacattg agacccaaat cttctggcaa
    12001 tatcagatat tagagtaggg tttttagttg ccgctgtatt aatagctacc ttatcggcac
    12061 cgcaacgcaa taattgcttg gcatcatcaa cactgcgaat tccgccacct acagtaattg
    12121 ggataaatac attttcagca gtagtcttta caatttctga caaattatta cgcccataca
    12181 aagatgcaac tgtatccata taaatcaatt catctgcacc ttgtgcataa tatgccatag
    12241 catattcatt gggatttcct actactctta atccctccag atgcactcct ttgattaggt
    12301 taggtccttt aatatctagt cgtgcaatta gacggacatt actcatcaaa cagtctccaa
    12361 atccagatat tcagctttag ttttttcaag atttgttact tgatgtctaa gtttccactg
    12421 ttctccatct tttttccaca agtgaggaga tctgaaggta tccgccaaag ccataaaata
    12481 ggctctgtcc ataatcggtt gctcgaacat ttgcgaagca atgggaaact ctttgaggtt
    12541 aatactcaag tatttaaaaa tttcttcagc aaaacgctct ggaaactctt gatcaaaacg
    12601 cttcaccaga gcaacacctt cttcacgagt aatatccccc gaacgtattt cctgggaggc
    12661 atcgtatgtt gctcttccaa taccgaattt ggttagcgtg gtgtagtaat ggaaatcatc
    12721 aattttgtca tcaatactat tgtatttact gtaggttcct ggagttcgtt caggagaagc
    12781 ttcaaaaccg ccatgttcca cagaataata ataacaactt tgaggatgcc acttcagata
    12841 ataacccaag taatgaacct ctacctgctg ctcttcgatt tgctgtggat cagcaggtaa
    12901 gtaagcatcc aaatcattgt catttaaacc gaaatcgctt tttaactctt gaacagatgt
    12961 accacctaaa aagattttgc ttttatcgtc cgcggtaaag tatttccagt ctctttttgc
    13021 agactccgta tcacctattg gattaccata ttccgcttca ttttctccat aaacaaccaa
    13081 tttaatttta tgcaacaaag ccattttagg agcataagct ttttgaccaa tcataaaggg
    13141 ttgaaatgga tggaatagat tctcaactgc gagtcgtgtc aatagcctgt gaactctgcc
    13201 atttggagta aacagatgat tatcaaatcc ggcatgtatc caggattgaa aattcctcca
    13261 accccatggc gtatacatat ggggagccca agtcacggtc aagggattca ttccgtattt
    13321 atattttaaa atgtgggcag cataaaaact atcctttcct ccggaaccag gaacaacgca
    13381 atcataagaa ccatccttgc ttctaaatcg atcacaaagc tctctcaatt gcctgtctcg
    13441 ctcttcccaa ttaatagtag attttttgcg ttcggcaact cgacaggcat cacaaatgcc
    13501 ttcgtcatca aaatggatgg tatgtttttt tgattctttc ttgtgctcat actctacagc
    13561 agaattaggc ctttgatttg aaataacaca gcttttgcaa aattccacct tatgtggtag
    13621 accatacttt gtttgtagct tttcatctgg gagctgaaaa cttgataaat caatggactt
    13681 tggtgcgtaa attttgctca tatcaattcc tatcctggca ttagacattt ttaaaaaacc
    13741 gcagtataca ataaaaattt caggtattaa acaaatcctg attgaagaac caaaatatat
    13801 aatttaataa atttgtgttt tcaatagtcg ccaattaccc ccttttaggg atatactgga
    13861 tgaatcgagt taaagtagga attttgcatg tccttgaagg ccatgtataa ccaaatcgca
    13921 gaaaattacg cgacagctaa tcgttttggt tcaattagtg aaagccatca cgttgccatt
    13981 gagcagatga ggaaatttca tttggggttg aaaccccatt ataaaatcct cgatctgggc
    14041 gttggcaatg gctctttttt gcaaaaattg catcacttaa tgccaatggc caatttcaca
    14101 ggaattgatg tttcatcaga aatgttgaaa cgagcgagcg aagcccttcc tttaacaacc
    14161 attgaaggca gtgctgcaga agccaacaag tttttgcccg cacatagcca ggacttggtt
    14221 ctggctcatt ttattaatgc atatatccct attaatgttc tctttgatga agccaaatac
    14281 ttgacgcgtg ccaatggtca cttctcttta atcacaacga cctatgattc ttttcctgta
    14341 gcgcaacaac aactggccaa cttcattgct caggatacta ttttaagtcg agttgttggt
    14401 cactactata aatccatagt caaaaataca actgttgctg caaatctcga tgaactcttg
    14461 ctggcattta aacagcatca atttaatatt ttggatcacc aacgtattga aatacccatt
    14521 acccttaata acattgatga attggcactg tttggaatag aaggcacctg gtttcttaac
    14581 actctatcga tacgcatgct gccaaaatat tttttaattc agcgtcttaa acgacttttc
    14641 agcaaaatct ttacttttcc ctaccatgac actcatatta ttgatgtagt tcttgccagg
    14701 aagtgataat cttgggcggt gggttagaaa aaacggaatg aggtgtgaaa ttgatttctc
    14761 aagcagttaa aaattatctt ccagaactaa gtcttcgcca aaaacaaacc aataatatta
    14821 tttttattac tttttggagc cagttttctg tctatgcctt gaatacagtt ctggttttat
    14881 ttttaactcg acccttgatt gctcagggat taggctacag ccaagccaaa gcctacgctt
    14941 tcattggtgt aactcaagcc acaggctacc tgatgcctat tttaggtggg tatatggctg
    15001 ataatattgt tggtgtcaga cgatccattc ttttgggtag catcatgctg gcttgtgctt
    15061 atttactggt catgttaagt gggtacacca tcagttctct tggggatcaa ttatttatca
    15121 tagcttttgc ctttattcct gcaaccaact cgctattaat gggaaccgca tccagtatgg
    15181 tttctcatat ttattccgat gatgccatta aagccaaatc cgctatgact tattactata
    15241 tggccattaa tgttggagct ctgctggcca ccatgatcgc gccagttctc ctggaaagtc
    15301 gttatggtcc actttcagta ctcaccttaa cctttattgg caaatcgatt gctgccttga
    15361 attttgcgaa acgttactcc atatatgata gtgtcatctg gggtatggat aaatccaaat
    15421 tttctaacca aggtctattg cgccttgttg cttatatagc ggctatctat tctcttactt
    15481 tatatgctta tactcatgtt tatatcgcca gcacactgat aggcgcagga tgtgctatag
    15541 gaattctctg gtttttaatt aaaaccatta tgcttaaagg cgaaactcgc agtaaacaaa
    15601 tgatcgctgt attactgata atagaagcca tagtattttt tatcatatac aatcaaatga
    15661 acagtacctt ggtattattt gcagaaaaca attctgatct aagttttctt tggtttaaaa
    15721 tttcacccgc tcaataccaa atgctaaatc ctttgctcat tttactgatt ggaagtcaac
    15781 ttccccgttt ctacaggttt ttttcgcgtt ttacgatccc ctatcaattt gccgcaggca
    15841 caattctctc tggtattgcc ctgctggtta tggcttttgc ggcccaaaat gcaatcaatg
    15901 gattagtgaa tggtaactac attgctttga cctacatttt aatttcaatt gctgaattat
    15961 gggtcagtgc cattggtctt agcatgattg gactgtattg tgatgcacgt gcgattgcct
    16021 ttgctatggg cgtatggtac ttggccagtt ccatgtccaa tgccatctca ggacgaattg
    16081 caggctgggt cgccatacca gagaatatta attctgctct tgaaagttta ccttattata
    16141 aaaattatta tttgattatg ggcattagtg ctttggggct tggtattctg atgtattttg
    16201 tagcttatta tttacacaag attatgaaac gacgaggtat tgagttagca taacgtaact
    16261 gaacattgta atattcgaat atgcttcgag acggtgcttc gcacctcctc agcatgaacg
    16321 gcctgatggg aaaatccaat tttgtaatca gccttggagt tttatgcaaa ccatcttccg
    16381 attatccata ggtttttctt gttaat
//
LOCUS       DAXLDT010000066        15987 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXLDT010000066 DAXLDT010000000
VERSION     DAXLDT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15987
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.066"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(106..1896)
                     /gene="oadA"
                     /locus_tag="ACF2SP_001692"
     CDS             complement(106..1896)
                     /gene="oadA"
                     /locus_tag="ACF2SP_001692"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HFD2359480.1"
                     /translation="MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAME
                     VWGGATFDACLRFLKEDPWSRLRQLRQVLPNTQLSMLLRGQNLLGYRNYADDVVRAFV
                     KLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELG
                     KKLAEMGCDSIAIKDMAGLLTPTVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEA
                     VLAGCNHIDTAISSFSGGASHPPTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKK
                     YSQFESEAQNIDPRVQLYQVPGGMISNLYNQLKEQNALDKMDAVHKEIPRVRKDLGYP
                     PLVTPTSQVVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRT
                     EVIEVRPGDLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEP
                     LLTQSSAPDNSVMSEFDIILHGESYHVKVAGYGMIEHGQQSCFLWVDGVPEEVVVQHS
                     ELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSAGDEVKAGQTVLVIEAMKMETEIK
                     APANGVVAEILCQKGDKVTPGQVLIRVEVS"
     gene            2098..2535
                     /gene="aroQ"
                     /locus_tag="ACF2SP_001693"
     CDS             2098..2535
                     /gene="aroQ"
                     /locus_tag="ACF2SP_001693"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HFD2359481.1"
                     /translation="MKKILVLHGPNLNLLGSREPSIYGHASLTEINGDLIQEADNAGI
                     RLSCFQSNAEAELIQAVHQAGIDKINYIIINPAAFTHTSIALRDALSAVAIPFIEVHL
                     SNIFSRETFRHHSYFSDIAVGVISGLGTKGYLLALQAIIKELK"
     gene            2549..3031
                     /gene="accB"
                     /locus_tag="ACF2SP_001694"
     CDS             2549..3031
                     /gene="accB"
                     /locus_tag="ACF2SP_001694"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFD2359482.1"
                     /translation="MDIRKIRKLIELLEETGISEIEIKEGEESLRLSRHSNIVAETAP
                     QIHYVAPSAVKQTAPTPSSNTSTTEPHVIENKQVTSASSGHKIRSPMVGTMYTAPSPE
                     SPPFVTVGQSVKAGDTLCIIEAMKMFNEIEADRAGKIVEILVKNGDPVEYDQALFIIE
                     "
     gene            3044..4387
                     /gene="accC"
                     /locus_tag="ACF2SP_001695"
     CDS             3044..4387
                     /gene="accC"
                     /locus_tag="ACF2SP_001695"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFD2359483.1"
                     /translation="MLSKIVIANRGEIALRILRACKELGIQTVAVHSDVDKDLLHVRL
                     ADETVCIGPAPAQKSYLNIPAIISAAEITDAVAIHPGYGFLSENADFADIVEQSGFRF
                     IGPRGDSIRLMGDKVSAIAAMKAAGVPCVPGSDGPLGDDDNKNLELARKIGYPIIIKA
                     AGGGGGRGMRVVHSESNLLSAIALTRSEAKAAFNNPIVYMEKFLENPRHVEFQVLGDG
                     KGHAIHLGERDCSMQRRHQKVVEEAPAPGITPELREEIGASVIKACKELQYRGAGTFE
                     FLFQDGCFYFIEMNTRIQVEHPVTEMITGIDLIKEQIKIASDIPLTLTQKDIKLRGHA
                     IECRINAEDPRNFMPSPGTIKLLHQPGGPGIRFDSHIYSSYVVPPNYDSMIGKLISYG
                     ATRAEAIARMRNALDEIIIDGIKTNIELHQRILHDKSFMEGGTNIHYLEKMLKDS"
     gene            4389..5258
                     /gene="prmA"
                     /locus_tag="ACF2SP_001696"
     CDS             4389..5258
                     /gene="prmA"
                     /locus_tag="ACF2SP_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFD2359484.1"
                     /translation="MWFQLKIEHCPNDKIEEITEELEEYGALSITLTDKNDNPVLEPE
                     PGTTPLWPEVIIHALFAQAEEAQYVREQLVAKRPSLHCSLELLADKNWERAWMDDFRP
                     QRFGNRLWVCPTWLPPPEPDAVNLMLDPGLAFGTGTHATTSLCLTWLEQADLKNKSII
                     DYGCGSGILSLAAIKLGAKHVYAVDIDNQALQATQSNAHANHITESQLSISTPEALQN
                     PVHLIIANILLAPLISLKERFHQLLPSGAHLVTSGILEEQAPLLIDAYDSAFTHIATE
                     YCEGWSLLVFTSK"
     gene            5285..6874
                     /gene="purH"
                     /locus_tag="ACF2SP_001697"
     CDS             5285..6874
                     /gene="purH"
                     /locus_tag="ACF2SP_001697"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFD2359485.1"
                     /translation="MAKKQIYSSFKPRRALLSVSDKRGIRELGQALHDQGVELIATGN
                     TAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIHAGLLARGEQDSPVLRQHGIKP
                     IDLLIVNLYPFEQVINHPDCDFNKAIENIDIGGPTMVRAAAKNHAHTYVIVDPNDYSK
                     LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFN
                     KITDLRYGENPHQQAIFYADKNSHPGSLSTAALLQGKQLSYNNILDADAALDCVKSFS
                     NEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAIL
                     EKQFVEVIIAPDANEEAKKILATKENIRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHD
                     SLSLESCELQTVTQVKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSR
                     VMSARIGLWQAEQMGFDPKGAVMASDAFIPFPDTIEIAAKAGISAIIQPGGSIRDEKI
                     ISCADQHNIAMIFTGVRHFKH"
     gene            7159..7944
                     /gene="icmH"
                     /locus_tag="ACF2SP_001698"
     CDS             7159..7944
                     /gene="icmH"
                     /locus_tag="ACF2SP_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFD2359486.1"
                     /translation="MTTEQYPSSLVNRLAITEPALVPSGYYRSKLFIAPFSTNVLVAA
                     AGPILSLLERLCLSPSLPPVEDIRENIQHELRAFHSKLDASKYPLDIISIAQYLLSAT
                     IDEILGKSYLRVYNLTTEFKSFTPLSSDGAQPQQRFFEILNYIKERPNQFLDLIELAY
                     FCLIAGFEGEYHLKADGRQCLDNTIEDLYQIIQKYRFNKPHRLFNENPLPKTIKKNYK
                     ATWITTIAAVGIVILAFVTSHVLLENKAKTVLFGHTQLAMLEN"
     gene            7944..10865
                     /gene="icmF"
                     /locus_tag="ACF2SP_001699"
     CDS             7944..10865
                     /gene="icmF"
                     /locus_tag="ACF2SP_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmF"
                     /protein_id="HFD2359487.1"
                     /translation="MDNSLRALCDAIKKILSQLKPQTNQLSFIVITGKNAQGKSALLK
                     QSNMEEMPVFSEEHAKLYFNQKGIIVELGENWLTNSKTLLLTTLKQLNKCNRHLKITG
                     LILCVDVNDLLIAEPAQFTEQKKSHLQLIQRLGVNLGYQAELALIFTKMDTLAGFSEF
                     YQMDHVNDLSKPLGFSLDCVNQLSKKIETFSLQFNQLIEALGQQVINKMHPARSTIKR
                     SLIREFPLQLASLRAPIQSLIQGISPKLFHVHSIFFTSAEQGGVSVDRLNKKIQHEYA
                     LVVQDTFPQATNFRSYFVEGALQTIQEQCIQVPQATKFSQKPFIAVAVSLAGLSLLTL
                     IYNHYKTAHILDEASKELLAYEALNNQGTSGAQALYHLSQAANKMNHITSNSISLPTV
                     HQLKLNLHNNAQNRLQGEFLPSLANELEQVITSPGNTPITRYKALKIYLMLGDSKYFS
                     SQPILDWFQQQWQGRAPGTTQKQLALLKQTLSNPPQNIPIKSQIISDARNYLNALPTS
                     YLYYSIAKEYFPQEKQKIVIEGFSIPTDEVPTYFTKTGFTKIINELPKITSTLQSENW
                     VLARQDLSQLHNMLIQAYCFDYVTWWQTFMKKSQPLRYQDYQQGRQLTKLIQQSNSFY
                     KFANLIQQETKPDLTEGISTFNQFIANQFTDLNLTTLSSIKELDFRISELERFIATLS
                     VVNDGGKTAFTITRSRFLNENSSDPVSLLYSQARQLPEPLSSWTKQIAGDTWVILIRD
                     SRQYINNQWQKTVYREFQNSIAQRYPFDSSQTAEIDIADFNRFFSTHGLLNTYSEQYI
                     KPFLDTSSPEWKPKAVNDFILPISNEVIDELIRANIITNMFFPDHSDESKIDFSLQKI
                     SLDPVVSNLILEIGGTKLMDNQTSDSLIRFTWPQNNAKLALDSIEGNHYELAEYGTWA
                     IFKLLEKVNVLVDEQDSSSLQILFEVNSNSGRYLLKTNNQINPFTPGILNGFTLNDHI
                     A"
     gene            10868..12124
                     /locus_tag="ACF2SP_001700"
     CDS             10868..12124
                     /locus_tag="ACF2SP_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359488.1"
                     /translation="MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHF
                     VSLIATFSLFAIGFIMRPIGGLLLGHYADHAGRRKALILSMSVMTISTALIAVLPDFN
                     SWGIIAPLLFSILRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
                     GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAEL
                     PAKVVFTRFKQQLFLSVIITSILAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALA
                     LLTVLIPLMGFLSDVIGRKPIFLIGLFSLCILIFPFFWLLLSGSWWKALCSELLLAIA
                     LAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPA
                     WYLFLWILIVIPITISIRESYQKELT"
     gene            complement(12222..15251)
                     /gene="icmB"
                     /locus_tag="ACF2SP_001701"
                     /gene_synonym="dotO"
     CDS             complement(12222..15251)
                     /gene="icmB"
                     /locus_tag="ACF2SP_001701"
                     /gene_synonym="dotO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmB/DotO"
                     /protein_id="HFD2359489.1"
                     /translation="MANWSESFFEGVDTFFAWLSTSLKQTTESYIDLETADSPTVLVN
                     HDGSLLSVLKIEGITGLAGPEEFERLVEGLANSFQAAMGRPGHALQVYFSHDKQNIVK
                     MIKDIYDPAEATASRLELNLRDLFEERVNYLSQYCAEERVYFVLYTRPFNLASDQLKA
                     ANKAKMKMIKDTKAPPFKNTQTIFAAVPEIRDTHDAYVRAILNDLDALNVIAKLLEVH
                     DAVHAIRMTGDPDYTADDWRATLPGDKIPVRELNNFEGDPSDLLWPPLSRQVLPRDAE
                     IIDLRTVRVGDKIYASTYIDLFPKDVRPFISLFSRILPSHVPWKISFLLESEGLSTIK
                     LKGLLAAILSFSSAQNRLISDSVNLLKYIQLNTDEAIVRLRVVATTWAPEGNIPLLRR
                     RSSELVKAIEGWGSTDVSEICGDPFAGFVSSMLATTLNSSAVASVAPLSDVISMLPIT
                     RPASPWKTGALLFRTPDGKPWPFQPGSTEQTTWIDLVYARPGSGKSVLSNALNLALCL
                     SGGLMRLPRIAIIDIGPSSSGLISLLKEALPASKRHLVAYHRLRMTPDYSINPFDTQL
                     GCRYPTALERSFLVNFMTLLTTPLGAAKPYDGMADLAGMVVDELYKSMADEFNPTPYA
                     PGIEEFIDSILEEIGFVRDAKSTWWEVTDSLYSAGFVHEAMLAQRYAMPLLADAASIC
                     RTPSIEDLYEKITAPTGESLINAFSRMISSAVREYPILSRVTSFDIGDARVVSLDLDE
                     VAKSGGDAADRQTAVMYMLARYVLARHYYLTEESLNNVPEQYKEYHKQRVLEIREDHK
                     RIVYDEFHRTAKSSAVREQVIIDMREGRKWKVQISLLSQAVDDFDPVMIDFATAIYVM
                     DAGPSQAVEKTSQIFGLSETAKIALRTRVHGPRQGGATFLAQFATKTGINVQLLTLTL
                     GPVELWAFSTTAEDATVRNLLYRHLGPSEARRLLAALFPNGTVTKELEARLASMKQKV
                     GLIEDDEREGMINQLVNDILDAYSKDPNVKSLPAKVG"
     gene            complement(15287..15913)
                     /gene="icmJ"
                     /locus_tag="ACF2SP_001702"
     CDS             complement(15287..15913)
                     /gene="icmJ"
                     /locus_tag="ACF2SP_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmJDotN"
                     /protein_id="HFD2359490.1"
                     /translation="MADNQQRCELKLIASPGSWRLYSARKIDERFKSYEQKIFQRDRY
                     TCQFCGFQARLYQDIVNLDGDYTNNRLSNLVTACCFCAQCFFVESVGVGGYGGGTLIY
                     LPELTQAELNSLCHVLFCAITNDTGYKSSAQNIYRSFKFRSQIVEEKFGEGTSDPAIF
                     GQLMIDSGVKSEEIREKLFKNIRLLPSRAKFRKQIEKWAASALEEIAD"
BASE COUNT         4982 a         3349 c         2887 g         4769 t
ORIGIN      
        1 caggttacta attaacctta ttgttatttt taataacata aaaattctta cataattttg
       61 gaataggtaa aaacaagtag tgaaaataat acacttaact acctgttaac tcacctccac
      121 tctgattaat acttgccctg gagtcacttt gtctcccttt tgacataata tttctgcaac
      181 caccccattt gcaggtgctt ttatttctgt ctccattttc attgcttcaa taaccagtac
      241 agtctgcccg gccttgactt catcaccagc agaaacatga attgcaataa tagaaccggg
      301 gatagctaca gtaatatctc caggtcctat cttgttatta acagatgaac gctctatttt
      361 gtcatgaagt tcagaatgtt gcacaacaac ttcttcagga acaccatcaa cccacaaaaa
      421 acaggattgt tgaccatgct cgatcattcc ataaccagct acttttacat gataactctc
      481 accatgaaga ataatatcaa actcagacat gacggaatta tctggtgcac ttgattgagt
      541 caataatggc tcaggaatta attgattatt tttacgctgc tctaagaatt ggcgcccaat
      601 ttcgggaaac attgcataaa gaagaacatc ctcatcactt agtgccaaat cacttatttc
      661 attttgcaat tgatctaatt cattaggtaa caaatcgcca ggcctgactt caatcacttc
      721 cgttcgtcca atcgcttttt tccgtaaagc agaactaatt ttgccaggag gagtgccata
      781 ttttccctga cagtagagtt tcacttcatt ggttattgtt ttataccgtt ctcccgttag
      841 aacattaata actgcctgag ttccaacgac ttgagatgtt ggcgtaacca aaggcggata
      901 acccaagtcc ttacgaacgc gggggatttc cttgtgaaca gcatccatct tgtctaatgc
      961 attctgctcc tttaactgat tatacaggtt tgagatcatg ccacctggca cttgatacaa
     1021 ttgaacacga gggtcaatat tctgtgcctc actttcaaat tgagaatact tcttgcggac
     1081 agctttaaaa taatcgtcta tttccaataa aatatttaaa tccaattccg tgtcatacgg
     1141 agtgtcagtc aatgcggcaa ccaatgcttc cgtaggtgga tgagatgcac cgccagaaaa
     1201 agatgaaatc gctgtatcaa tgtgattaca acccgctaaa acggcctcat aatggcatat
     1261 acttgccaaa ccggaagtag aatgactatg caaatgtaca ggtaagccag tcgcttgttt
     1321 taaaccagca tacaattcga cagtcaccgt tggggttagc aaacctgcca tatcttttat
     1381 ggcgatacta tcacatccca tttcagccaa ttttttccct agctccaaaa aattgtccaa
     1441 agtatgaacc gggctggttg tgtaacaaat tgctccctgg gcatgttttt tatgtgattt
     1501 aatcgcgtct atggctactt taaggtttcg cgcgtcattt aaagcatcaa acacacgaaa
     1561 tacatccact ccattattca cggccaattt aacaaaagct cggactacat catctgcata
     1621 attcctatag cctaacaaat tttgacctct taacagcata gagagttgag tattcggtaa
     1681 cacttggcga agttgacgca atcttgacca gggatcttcc tttaaaaatc gcaaacaggc
     1741 atcaaaagta gcgcctcccc agacttccat agcccaaaat cctacatcat ccattttatt
     1801 acagattggc aacatatctt cagtgcgcat tcttgtagcg attaagcatt gatgggcatc
     1861 acgcaaggtg acatctgtta taaacgtttt agacataagc tcttcctggc tcaagaaata
     1921 aataaaaata tcataatcct aaaatcggta cactctttaa gtacaatcca ttgcttttta
     1981 ctaaatataa aatttcttgc taaacttgcg gagatcttgc cgatatcctc gcagatatat
     2041 agacatgttc ataaagttca ggcaaaatct gcaaatctca gactatactg atgtgtaatg
     2101 aaaaaaattc tagtattaca tggtcctaat ctaaaccttt taggctcccg agaaccgtcc
     2161 atttatggac atgcttcgtt aactgaaatc aatggtgatt taatccagga agcagacaat
     2221 gcagggatta gactcagctg ctttcaaagc aatgctgaag cagagttaat tcaagctgtc
     2281 catcaggctg gaattgataa aatcaattac attatcataa atcctgcagc atttactcac
     2341 acaagcattg ctcttagaga tgctttaagt gcggttgcca tcccatttat tgaggttcat
     2401 ttaagtaaca ttttttcccg tgaaactttt cgtcaccatt cctatttctc agatattgcc
     2461 gttggtgtca ttagcggttt ggggacgaaa gggtatttac tggccttaca agcgattatc
     2521 aaagaattaa agtagagaga aaaaacaaat ggatatcaga aaaatcagaa aacttattga
     2581 actcctggaa gaaactggta tttcagagat tgaaatcaaa gaaggagaag agtcgttaag
     2641 actaagtcgg catagtaata tagtagcaga aacagctcca caaattcatt atgtggctcc
     2701 ttctgctgtg aagcaaacag cccctactcc ttcctccaat acttctacca ccgaacctca
     2761 tgtgattgaa aataaacaag ttacttcagc ctcctcaggt cataaaattc gttcaccaat
     2821 ggttggaaca atgtatacag cgccatctcc tgaaagccca ccttttgtaa cagtgggaca
     2881 atctgttaaa gcaggagata ctctatgcat tattgaagca atgaaaatgt tcaatgaaat
     2941 tgaagccgat cgtgctggga aaattgttga aatcttggta aaaaacgggg atcctgttga
     3001 atatgaccag gccttgttta ttatagaata gaggggcctg tttatgctca gtaaaatagt
     3061 tattgctaat cgaggcgaaa ttgcgcttcg tatactgcgt gcttgtaaag aacttggcat
     3121 tcagactgtt gctgttcact ctgatgtaga caaagattta ttgcacgttc gcctggctga
     3181 tgaaaccgta tgcataggcc ctgcacctgc ccaaaaaagc tatctcaata ttcctgcaat
     3241 tatatctgcg gcagaaatta ctgatgcagt agcgatacat ccaggttatg ggtttttatc
     3301 agaaaatgcc gattttgcag atattgttga gcaaagcggt ttccgtttta ttggcccacg
     3361 cggcgactcc attcgtctta tgggggataa agtttccgcg attgctgcca tgaaagcggc
     3421 aggagtaccc tgtgttcccg gttcagacgg tcccttagga gatgacgaca acaaaaatct
     3481 ggagttggcg cgaaaaattg gttatccaat cattatcaaa gcagccggcg gcggtggtgg
     3541 acgtggcatg agagtcgttc atagcgaatc caatttactt agtgctattg cgttaacccg
     3601 aagcgaagca aaagccgctt tcaataaccc gatagtttac atggaaaaat ttctggaaaa
     3661 tcctcgccat gttgaattcc aagtccttgg tgatgggaaa ggacatgcta tacatttggg
     3721 tgagcgtgac tgctcgatgc aaagacgaca tcaaaaagtc gtagaagaag ccccggctcc
     3781 aggaatcact cctgaattaa gagaagaaat tggcgcgtca gtgattaaag cttgcaaaga
     3841 gttacaatac cgaggtgctg gaacctttga atttttattt caagatggtt gtttttattt
     3901 catcgaaatg aatacgcgca ttcaggttga gcatccagta actgaaatga tcactggtat
     3961 tgatttaatt aaggaacaaa tcaaaattgc cagcgatatt cctctgacat taacccaaaa
     4021 agatattaaa ttacgcggcc atgctattga atgccgaatc aatgcggaag atccaaggaa
     4081 ttttatgccc tctcctggaa ccataaaatt acttcatcaa ccaggaggcc caggcattcg
     4141 ttttgattct cacatctaca gcagctatgt tgttccacct aattacgatt ccatgatagg
     4201 aaaattaata agttatgggg caacaagagc agaagcgatt gccaggatgc gtaatgctct
     4261 tgatgaaatt atcattgatg gcataaagac aaatattgaa ttacaccaac gtattcttca
     4321 tgacaaatct tttatggaag gtggcacaaa cattcactat ctagaaaaaa tgctcaagga
     4381 ttcatagcgt gtggtttcaa ctaaaaatag aacattgtcc taatgacaag attgaagaaa
     4441 taaccgagga actcgaggaa tacggtgcct tgtccatcac actcacggac aaaaatgaca
     4501 accccgttct tgaaccagag ccagggacaa cgcctttatg gcctgaagta atcattcatg
     4561 ccctatttgc ccaagcagaa gaagcgcaat acgtaagaga acaattggtt gctaaaagac
     4621 catctctgca ttgcagtttg gaactgctcg ctgataaaaa ttgggaaaga gcctggatgg
     4681 atgattttcg acctcaacgt tttggtaacc gcttatgggt ctgtcctact tggctccctc
     4741 ccccggaacc tgatgcagtt aatttaatgt tagatcctgg gttagcattt ggtacaggca
     4801 cacatgcgac aacctcccta tgcctgacct ggctggagca ggctgattta aaaaataaat
     4861 caataataga ttatggttgt ggttcaggga tactctcact cgcggcaatc aagttaggag
     4921 ctaaacatgt ctatgctgtt gacatagata atcaagcgtt acaagccact caaagtaatg
     4981 cgcatgccaa tcacatcact gagagccaac tctctatcag caccccagaa gcattgcaaa
     5041 atcctgtgca tttaattatc gcaaatattt tgttggcccc attaatcagt ttaaaggaac
     5101 gttttcatca actgctcccg tcaggcgcgc atttggtaac atcaggaatt ctggaagaac
     5161 aagccccttt gctgattgat gcttatgatt ccgcattcac tcatattgca actgaatatt
     5221 gcgaaggatg gtcgttgctg gtttttacaa gcaaataata actatatcac taaggtatat
     5281 gtaaatggcg aaaaaacaaa tttactcttc tttcaagcca cgacgagcac tactgagtgt
     5341 ttccgataaa agaggaataa gagaactcgg tcaggcctta cacgaccaag gagtggaatt
     5401 gatagccaca ggaaacacag ctgcgatctt aagggaacat aaattaccag ttaccgatgt
     5461 cagtgagtgc acgggatttc ccgaaataat ggatggaaga gtcaaaaccc ttcatccagc
     5521 tattcatgct ggtttattag cccgcggaga acaagacagc ccagttttaa gacaacatgg
     5581 aataaaacct attgatttgc tgattgtcaa tttatacccc tttgaacaag tcattaacca
     5641 cccggactgc gattttaata aggccattga aaacattgac attggtggac caacaatggt
     5701 gcgagcggca gctaaaaatc atgcccatac ttatgtcatc gttgatccaa atgattactc
     5761 aaaattaatt cattatttac aagatcagaa agcaccttct cattggaatt ttgcgttagc
     5821 taaaaaagca tttgcccata ctgcagccta cgatgccgca attgcaaatt atttgaccac
     5881 tttggataat gattatgttc caactggatt tcccgacata ctaacctgcc aatttaataa
     5941 aattactgat cttcgttatg gtgaaaatcc ccatcagcaa gctatttttt atgctgataa
     6001 aaattcacat ccaggctctc taagtacagc agccttatta cagggaaaac aattatccta
     6061 taataatatc cttgatgccg atgcagctct cgattgtgtg aaatcatttt ccaatgaaaa
     6121 gtcagtttgc gtcattgtaa aacataccaa cccctgcggc attgcactat ccgacacatc
     6181 acttgacgct tacttaaaag cctttcaaag tgatccgata tccgcttatg gtggaattat
     6241 tgctttcaat ggaactctgg atagcgatac ggccaaagca atattggaaa agcaatttgt
     6301 ggaagtgatt attgcacctg atgccaatga agaagcaaaa aaaattctgg caaccaaaga
     6361 gaacattcgt gttcttctaa ctggtttttg gcaacaaggt aataatttta gattaagtat
     6421 gaagaaagtc gatggtggtc tattagtaca agaacacgat tctctttctc tggaatcatg
     6481 tgagttacaa accgttaccc aagtaaaacc cactgatgaa caactacaaa atttaatgtt
     6541 cgcttggtta gcagccaagc atgtcaaatc caatgccatt gtatacgcta atgatttggc
     6601 cactataggg attggcggag gacaaaccag tcgagtaatg agtgctcgta ttggcttgtg
     6661 gcaagcagag caaatggggt ttgatcccaa aggcgctgtt atggcctcag acgcatttat
     6721 ccctttccct gatactattg aaatagctgc gaaggctggt atatccgcaa ttattcagcc
     6781 agggggttct atcagagatg aaaaaattat ttcttgcgcc gaccaacaca acatagcgat
     6841 gatttttaca ggagtgcgac attttaaaca ttaatgtcaa gcatgtatta attctaagct
     6901 tgactaactt gaacaaagag tcgtatataa cgtatcatct tctttttaat tgaagctttt
     6961 tgattagcat aaatggacct tttcttgtag gactgatgga cttgatatta gaacaaggag
     7021 caagtatttc tagaagcaga ttttacctat agaataaata agaattatag gaatacttac
     7081 taatcaactg gcaatcacgc aaaagacaaa aatgctactg aaagtctaat gcgtcactga
     7141 aacccatgga gtaatataat gacaactgag caatacccgt cctcacttgt caatcgtctt
     7201 gcaatcacag agcctgcgtt ggttccaagt ggatattatc gttcgaaatt atttattgcc
     7261 ccattttcta caaatgtttt agttgcagcg gccggaccaa tactgtcttt attggaaaga
     7321 ctttgtttaa gtccttcttt acctccagta gaggatataa gagaaaatat acagcatgaa
     7381 ttacgcgcct ttcacagtaa attagacgct tctaaatatc cgttggatat tattagcatt
     7441 gctcaatatt tattatcagc taccattgat gaaatattag gtaaaagtta tctaagggtc
     7501 tataacctga cgacagagtt caaatcattt actcccttat caagtgatgg ggcacaaccc
     7561 cagcagcgct tttttgaaat attgaattac attaaagaga ggccaaatca atttctcgat
     7621 ttaattgaat tagcctactt ttgcctcatt gccggatttg agggtgagta ccatctgaaa
     7681 gcagatgggc gacaatgtct tgataatact attgaagatt tatatcaaat catacaaaaa
     7741 taccgtttta ataaaccaca tcgcttgttt aatgagaatc ctcttcccaa aactattaaa
     7801 aaaaattata aagcaacgtg gataacaact attgctgctg taggtattgt aattctagct
     7861 tttgttacta gccatgtttt attagaaaac aaagccaaaa ctgttttatt tggacatacc
     7921 caacttgcta tgctggaaaa ctaatggaca attcattacg cgcattatgt gatgctataa
     7981 aaaaaattct ctctcaatta aaaccgcaaa ctaatcaatt atcttttatt gtcattaccg
     8041 gaaaaaacgc ccaaggcaaa tcggcattgt taaagcaaag taatatggaa gaaatgcccg
     8101 tattcagcga agagcatgcc aaactatatt ttaatcaaaa gggaatcata gtagagcttg
     8161 gtgaaaattg gcttaccaat agcaaaacat tattgctcac aacacttaaa caattaaata
     8221 aatgcaatcg tcatctcaaa attacaggct tgattttatg tgtggatgtc aatgatttac
     8281 tgatcgccga accagcacaa ttcactgaac aaaaaaaatc tcatttgcaa cttattcaac
     8341 gtttaggtgt taaccttgga tatcaggcag aattagcttt aatattcacc aaaatggata
     8401 ccttggctgg cttttctgaa ttttatcaaa tggatcatgt caatgatcta tccaaacctc
     8461 ttggtttttc ccttgattgt gtcaaccaat taagtaaaaa aattgaaaca ttttcccttc
     8521 aattcaatca attaattgaa gctttgggac agcaagttat caataaaatg cacccggcac
     8581 gttccactat taagcgcagt ttgatacgcg aatttccttt gcaattagcc agcttgagag
     8641 ctccaattca gtcgttaatt caggggattt cgccaaaatt attccatgtt cactctattt
     8701 ttttcacgag tgccgagcaa ggtggagtca gtgtagatag gctaaacaaa aaaattcagc
     8761 atgaatacgc tttggttgtt caggatactt tccctcaagc gaccaatttc agatcctatt
     8821 ttgtagaagg tgcactgcaa acaattcagg aacaatgtat ccaagtacca caggctacaa
     8881 aattttctca aaaaccattc attgctgttg cagtaagttt ggccgggctc agtttattaa
     8941 cgctgattta taaccactac aaaaccgctc atattcttga tgaggctagt aaagaattac
     9001 tggcctatga agcactgaat aatcagggca ctagcggtgc acaagcgcta tatcacttat
     9061 cgcaggctgc aaataaaatg aatcacatta catcaaactc catatcactg cctactgtgc
     9121 accaattaaa attaaacctt cacaacaatg ctcaaaatcg tttacaagga gaatttcttc
     9181 cctccttagc caatgagtta gaacaagtca tcacaagtcc tggcaacacc cctatcactc
     9241 gttataaggc tttaaaaatt tatttaatgt taggagactc aaaatatttt tcatcccaac
     9301 ccattctgga ttggtttcaa caacaatggc agggacgtgc accaggcact acacaaaaac
     9361 agttggctct tttaaaacaa actcttagta atcctcctca aaatataccc atcaaatcac
     9421 aaataatcag tgatgcgagg aattatctta atgcacttcc aacaagctat ttatattact
     9481 ctattgctaa agaatatttc cctcaggaaa aacaaaagat tgtgatcgaa ggtttttcta
     9541 tacccacgga tgaagtgcca acctatttta ctaaaaccgg atttacgaaa attattaacg
     9601 aattacctaa aattaccagc acacttcaat cagaaaactg ggtacttgcg agacaggatt
     9661 tgtctcaatt acacaatatg ctgattcagg cttattgttt tgattacgtc acttggtggc
     9721 aaacgttcat gaagaaatcc caaccattac gttatcagga ttatcagcaa ggccgtcaat
     9781 taacaaaatt aatacaacaa tccaattcgt tttataaatt cgctaatctt attcagcagg
     9841 aaaccaaacc ggatttaacc gaaggcatat ccacgtttaa tcaattcatt gccaatcaat
     9901 ttactgattt aaatttaaca actttatcca gcatcaagga attagatttt agaatttctg
     9961 aattagaacg atttattgca actttatctg ttgtaaatga tggcggtaaa acagcgttta
    10021 ccattacacg ctcgcgtttt ctcaatgaaa attcctctga ccctgtgagt ttgctctaca
    10081 gccaggcacg acaattacct gaacctttga gttcgtggac aaaacaaata gctggtgaca
    10141 cttgggttat actgattaga gatagtcgtc aatacattaa taatcaatgg caaaaaaccg
    10201 tctatagaga gttccaaaac tctattgccc agcgctatcc ttttgactcg tcacaaactg
    10261 cagaaataga tattgcagat tttaatcgct ttttctcaac acatggctta ttgaacacct
    10321 actctgaaca atacattaag ccatttcttg acacttcatc tccagaatgg aagcctaaag
    10381 ctgtgaatga tttcatcttg cctatttcaa atgaagtaat tgatgagtta attcgtgcca
    10441 atatcattac taatatgttc tttcctgatc acagtgatga aagcaaaatt gattttagct
    10501 tgcaaaaaat ctctctggat cctgtagttt ctaacctaat ccttgaaatt ggcggcacta
    10561 aattgatgga taatcaaaca agcgattcac taattcgctt cacctggcct caaaacaacg
    10621 ctaaattggc tcttgattct attgaaggga atcattacga gctcgcagaa tatggaacct
    10681 gggcaatatt taaattactt gaaaaagtta atgtgcttgt ggatgaacaa gatagttcca
    10741 gcctccaaat actttttgaa gtgaatagca actccggccg ctatttatta aaaacaaaca
    10801 accaaattaa tccttttact ccaggaattc tgaatggctt tactttaaac gaccatattg
    10861 cataattatg aagcaaaata ccaaacacag cattattgct ggattatacg gaaatgcttt
    10921 ggagtggtat gatttcttgc tctatgccag ttttgctcct gtctttgctg aaattttttt
    10981 cccttcaaaa atccattttg tttctcttat agcaacattt agcttatttg ctataggatt
    11041 cattatgcgt ccaattggtg ggcttttgct tgggcactat gctgatcatg caggacggcg
    11101 taaagcatta atattatcta tgtcagtaat gacaatttct accgcattaa ttgcggtatt
    11161 acctgatttt aattcgtggg gaattatcgc cccactttta ttttctattt tgcggctcat
    11221 tcagggtctt gcagtgggcg gcgaactgcc tggctcgacc acctatttga ttgagcatat
    11281 gtttcaacaa agccgggggc tcgctggcag cctcgtgtta agtagcgctt ttttaggtat
    11341 ttttattgga gcgcttaccg catcaatatt cagcacatta tttacaggtg atgctctatt
    11401 aaattggggg tggaggtatg cgtatctgtt aggaggcatt ttaggaattt tgggcattta
    11461 tttgcgtatt aaaagctttg aatcacccac atttctaaag gataaacata ctgctgaact
    11521 tcctgcaaaa gtggtattca cgcgatttaa acagcaactg tttttatctg tgataattac
    11581 tagcattctt gccatgtcta attatgtact tatcgcctat gtcacaactt ttttagtcaa
    11641 atcagagtat tttctcttga aagatgcgtt attaatcaac ttaattgccc ttgctctttt
    11701 gacagtttta ataccgctga tgggatttct ttctgatgtt ataggacgca agcctatttt
    11761 cttaattggt ttattttctc tttgcatatt aatcttccca tttttttggc tcttattaag
    11821 tggtagttgg tggaaagcat tatgcagcga attattacta gcgatagctt tagccccaat
    11881 caatgcgaca gtacccacta ttattgcaga aatgtttcca accagtgtac gggcgagtgg
    11941 gatttccata tcctataaca ttggccaggc tttatttggc ggtaccatgc ctttggttgc
    12001 gtttaccctg attgaattaa gtagcaataa aatggctccg gcttggtacc tgttcctatg
    12061 gatccttatc gtgataccaa taactatttc cattcgtgaa agctatcaaa aagaacttac
    12121 ttaaaacatt agatttaaat cagtttttta gcagaaaaaa tccagcatta atagccagaa
    12181 aataagtgca ttgagacata ctaataaaaa tcttacctgc ttcaacctac ttttgcaggc
    12241 aagcttttca cgttgggatc ttttgaataa gcatcaagaa tatcattcac caattggtta
    12301 atcattccct ctctctcatc gtcttcaatt aacccaactt tttgcttcat gcttgctaat
    12361 cgagcctcca attctttagt cactgtacca tttgggaaca gtgcagctaa aagccgtcga
    12421 gcttcacttg gtcccagatg cctatacaaa agatttcgta ctgtggcatc ttctgcagta
    12481 gtactaaatg cccataattc caccggcccc aatgttaagg ttaataattg tacgttgatg
    12541 cctgttttag tggcaaactg agcaaggaaa gtagcaccac cttgtctagg accatgaact
    12601 cgagtacgta aagcaatctt ggctgtttca gaaagtccaa aaatttgact ggttttctcg
    12661 acagcttgag atggtcctgc atccataaca taaatagcgg tagcaaaatc aatcattacc
    12721 ggatcaaagt catctactgc ttgtgataat aaggaaattt gcactttcca ttttcggcct
    12781 tcacgcatat caataataac ttgctcacga acagcagacg attttgccgt gcgatggaat
    12841 tcatcataaa caatacgctt atgatcttct cgaatctcta atactctctg tttgtgatac
    12901 tctttatatt gttccggtac attattcaaa ctttcttctg ttaaataata gtgtcttgcc
    12961 aatacgtatc tggctagcat atacataacc gcagtttgtc tatcagccgc atcaccaccg
    13021 cttttcgcta cttcatccaa atcaagagaa actactcttg cgtcaccaat atcaaaacta
    13081 gtgacccgtg ataaaatagg atattcgcga acagccgatg aaatcattct tgaaaaagca
    13141 ttaattagtg attcaccagt tggagccgta atcttttcat acaaatcctc aattgatggt
    13201 gttctacaga tagaagcagc atccgctaat aacggcattg catatctttg cgccaacatc
    13261 gcttcatgga caaaacctgc tgaatagagc gagtcagtca cttcccacca ggttgatttg
    13321 gcatctcgga caaaacctat ttcctccagg atgctatcga taaattcctc tattcctgga
    13381 gcataaggag tcggattgaa ttcatcagcc atgcttttat ataattcatc taccaccata
    13441 cccgccaaat cggccatacc gtcatatggc tttgcagcac ctaatggcgt agttagcaag
    13501 gtcatgaagt tgactaagaa tgatcgttcc aaagcagttg gataacgaca tcccaattgg
    13561 gtatcaaagg gattaatgga ataatctggg gtcatcctta atctatgata agcaaccaaa
    13621 tgacgtttgg atgcaggaag agcctcttta agcaatgaaa tcaaaccact acttgaggga
    13681 cctatatcaa taattgcaat gcgcggcaaa cgcattaaac cgcctgaaag acaaagagct
    13741 aaattcagtg catttgacaa cacagattta ccagaaccag gacgagcgta aaccagatca
    13801 atccaggtag tttgttcagt tgagccaggt tgaaacggcc acggcttacc atctggtgtt
    13861 ctaaaaagta aggctcctgt tttccaggga gatgctggtc ttgtaatagg cagcattgaa
    13921 ataacgtctg acaaaggagc tactgatgct actgctgagc tgttcaaagt agtagctagc
    13981 atacttgaca caaagcctgc aaacgggtcc ccacaaatct ctgatacatc agtagaaccc
    14041 caaccttcaa tagcctttac taactcagaa cttcttcgcc gcaacaaggg aatattgcct
    14101 tcaggggccc aggttgttgc aacgacgcgt agtcttacaa tcgcctcatc cgtattgagt
    14161 tgtatatatt tcaacagatt aaccgaatca ctaattaaac gattctgtgc ggagctaaag
    14221 ctcaatatcg ctgccaagag cccctttaat ttaattgtgc ttaatccttc gctttctagc
    14281 aaaaaagaaa ttttccaggg cacatgtgaa ggaagaatac gagaaaataa actaataaat
    14341 ggtcttacat cctttggaaa caaatcaata taggttgaag cataaatttt atcgcccact
    14401 cggacagtgc gtaaatcaat aatctctgca tctcttggca aaacctgcct ggacaacggc
    14461 ggccataata aatcagaagg atccccctca aaattattca gctcgcgaac aggtatttta
    14521 tctccaggta aagttgcgcg ccaatcatct gcagtataat caggatcacc tgtcatgcga
    14581 atggcatgta ctgcatcgtg tacctcaagc aacttagcta tcacattcag ggcatccaaa
    14641 tcatttagaa ttgcacgcac ataagcatca tgtgtatctc ttatttccgg aacagccgca
    14701 aaaatcgttt gtgtgttttt aaaaggagga gcctttgtat ccttgatcat cttcattttt
    14761 gctttgttcg cagcttttaa ctgatcgctc gctaaattaa atggtctcgt atacaaaaca
    14821 aaataaacac gctcctctgc acaatattgt gataaataat tgaccctctc ctcaaataaa
    14881 tctcttaaat taagttcaag acgacttgct gttgcttccg cagggtcata aatatccttg
    14941 atcattttta ctatgttttg cttatcatgg ctaaaataaa cttgtagcgc atgaccaggt
    15001 ctacccatag cagcttgaaa tgagttggct aaaccttcaa cgagtcgttc aaactcttca
    15061 gggcctgcca atccagtaat cccttcaatt tttaatactg acagtaatga tccatcatga
    15121 ttaactaata cggtagggct atccgccgtt tccaaatcta tataagactc tgtagtttgc
    15181 ttcagggaag tacttaacca ggcaaagaaa gtatcgacgc cttcaaaaaa cgattctgac
    15241 caatttgcca tcctttgtcc tcaattatta atagtacttt tactgtttaa tctgcaattt
    15301 cttctaaagc actggcagcc catttctcta tttgcttcct gaattttgcc ctagagggta
    15361 atagccttat atttttaaaa agtttctctc ttatttcttc acttttcaca ccagaatcaa
    15421 tcattaattg cccaaatatt gcagggtcac ttgtgccttc accaaatttt tcttcgacaa
    15481 tttgcgagcg aaatttaaag cttctataaa tattctgtgc actggatttg tatccggtat
    15541 cattggtaat agcacaaaaa agaacatggc ataagctatt tagttcagct tgggttaatt
    15601 ctggcaggta gatcaaagta cctccaccat aaccaccaac tcctactgac tcaacaaaaa
    15661 agcattgtgc acaaaaacaa caagctgtaa ctaaatttga taacctattg tttgtataat
    15721 caccatccag attcacgatg tcttggtata atcgggcttg aaacccgcaa aattgacaag
    15781 tatacctatc tcgctgaaag attttttgct cataggattt gaatcgctca tcaatttttc
    15841 tggcagaata taaacgccag gaacctggtg aagcaattag ttttagctca catctttgtt
    15901 gattatccgc catattttgg ttcacattca gtcaattttc aaaaaattat gtgttctatc
    15961 cttaactact gactaagcca gcttaaa
//
LOCUS       DAXLDT010000067        15803 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXLDT010000067 DAXLDT010000000
VERSION     DAXLDT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.067"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            143..1714
                     /locus_tag="ACF2SP_001703"
     CDS             143..1714
                     /locus_tag="ACF2SP_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HFD2359491.1"
                     /translation="MRRQWYIGFGVILLCLFALFAMLQDIVWFFAFLIPIVILWIYDV
                     LQTKHTILRNFPVLGHVRYFLEFLRPEIQQYFIATDESELPFNRETRSMIYQRAKNVR
                     DTIPFGTERDILSVGYTWALHSLSPKHSSEVEPRIDVGGPDCKKTYNASRLNISAMSF
                     GSLSSNAIMALNKGAKLGGFYHNTGEGGLSPYHLQGGDIVFQIGTAYFGCRDDKGDFD
                     ENEFIKEASRDEVKMIEIKLSQGAKPSHGGILPAAKLTEEIAKVRKVPMGKDVLSPIA
                     HTAFDNPVGLLHFVKRLRDLSGGKPIGFKLCLGRRHEFMAICKAMLKTNILPDFITID
                     GAEGGTGAAPVEYTNFIGTPLEAGLVFVHNALVGTGVRDKIRVICSGKVTNGFDLLTN
                     IALGADICNSARAMMMSIGCIQSKQCNANTCPTGVATQKKRLQYGLVVDEKKFLVANF
                     HNNTIKSFLEMVGALGLNNPSDLKPSHIMRRIGVDEVKSFDQIYTYVKPGQFLQGDIP
                     DDYRVHWEHADPDKF"
     gene            complement(1806..3419)
                     /locus_tag="ACF2SP_001704"
     CDS             complement(1806..3419)
                     /locus_tag="ACF2SP_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFD2359492.1"
                     /translation="MNIKSNLAHSSVILKGHVNRYAFLGLLISVGSILIASCIVSYQL
                     TGSVSLGGFIQAQRSNPAIWILDLTPFMFVYWGQAFCYGLVNKAESLLVDKTKELLNI
                     SGNLELKLKYESNHDSLTNLPNNRLLNEKIRLAIEQLGAQGELAVIVIKINDLNYNFS
                     SFNANNIVKQFAEKLKSILIDPYMLKASLGINMVARLQSDEFAILMPRLKKDLDINEL
                     LIHLLTLLNNNLMVDGISINVTSTVGAAIYPVHGEEDDVLVNHAIIAVYQARKENKSY
                     AIYSPEMEEDLIQNRMVMNELQRSIENKDLKIYYQPIVELANGQIVGAESSARFEHPE
                     LGLLSAEKFMPLIEGTNLIHNLTTLMLKEVIKQLAAWHDAGHKIFVSVNLSVHHELPE
                     LIEKLLNDYEIAPQFLKLEFTERACLTEQMVTKEVFDRLSTMGIKLCIDDFCSENSSF
                     IHLTNFPIENIKIEKSFVLKIAKDAKKAKIVEAIVKLAQTLGWEPLADGIADQNALEK
                     LRDLGCLYGQGLYFSRAVNVAEFTSLLKK"
     gene            3617..3779
                     /gene="ssrS"
                     /locus_tag="ACF2SP_001705"
     ncRNA           3617..3779
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACF2SP_001705"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3774..5765)
                     /gene="uvrB"
                     /locus_tag="ACF2SP_001706"
     CDS             complement(3774..5765)
                     /gene="uvrB"
                     /locus_tag="ACF2SP_001706"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFD2359493.1"
                     /translation="MKDLFKIYSNYQPAGDQPTAIASLIDGLESGLAKQTLLGVTGSG
                     KTFTIAHVIQAMKRPTLIMAPNKTLAAQLYGEFKAFFPDNAVEYFVSYYDYYQPEAYV
                     PASDTFIEKDASINEHIEQMRLSATKALIERKDAIIVATVSAIYGLGDPDSYLRMLLH
                     LSRGEQSDQRKILKRLAEMQYTRTNLSLERGQFRVHGDVIDIFPADSEKEAIRIELFD
                     DEVDNIARFDPLTGEILQRLPRVTIFPKTHYVTPRERILETVEKVKVELQERLAELNA
                     QNKLVEAQRLEQRTCFDIEMMLELGYCSGIENYSRYLSNREAGEAPPTLFDYLPPEAL
                     LIIDESHVTVPQIGGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEERSPQTIYI
                     SATPGPYEQEHSDNVAEQVVRPTGLIDPEVEIRPVKTQVDDLMSEIRQVIAQGSRILV
                     TTLTKRMAEDLTEYLSEHGIKVRYLHSDVDTVERMEIIRDLRLGEFDVLVGINLLREG
                     LDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNVKGRAILYADTMTGSMQRALTE
                     TERRREKQKAFNLKHGITPKGINKSVEDILEGAYIGKRKTMVAEQAPRYTHWSPQELA
                     KEINALEKQMYAHAQNMEFELAAKIRDEYLLLKEQLMKI"
     gene            5812..6990
                     /locus_tag="ACF2SP_001707"
     CDS             5812..6990
                     /locus_tag="ACF2SP_001707"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFD2359494.1"
                     /translation="MDIALAKRVQKVKPSPTLAVAAKAAQMKAQGLDIIGLGTGEPDF
                     DTPQHIKLAAISAIEAGDTKYTAVDGIVELKEAVKNKFKRDNELDYQLNQILVSVGGK
                     QSCYNLCQAYLNPGDEVIIPAPYWVSYPDMVLLADGVPVIIETTPAQRYKINAQQLEQ
                     AITPKTRMIFLNSPSNPSGIAYTQNELKELGDVLKKHPQILIATDDMYEHIIWSQAFT
                     NILNACPELYDRTIVLNGVSKAYAMTGWRIGYAAGPAPLINAMKTIQSQSTSNPCSIA
                     QRAAVAALNGSNESIEEMVNAFHQRHDYVADRLQSIDGIEVIPADGTFYIFPSVQAII
                     EKRGYANDIEFSEKLLNEVGVALVPGSAFGTEGCIRISFATGIDTLKDALNRLQRFCS
                     "
     gene            7050..7125
                     /locus_tag="ACF2SP_001708"
     tRNA            7050..7125
                     /locus_tag="ACF2SP_001708"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7083..7085,aa:Asn,seq:gtt)
     gene            complement(7544..8293)
                     /locus_tag="ACF2SP_001709"
     CDS             complement(7544..8293)
                     /locus_tag="ACF2SP_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISLpn10 family transposase"
                     /protein_id="HFD2359495.1"
                     /translation="MHHNISEELWSKIYSYLRGLKGLHVKNEASLRRFIEGVWYVLRT
                     GCQWRLLPYYYGHWRQIHRRYKVWSDRGIWSELMSVVSDVDIQTMMIDGTIVRAHACA
                     SGYVKDGNETQALGRSKGGFTTKIHALVDGLGNPIKFILTGGHRNDITQAAELLKEQH
                     DTIVLADKGYDSQELIDTLHHQRCSPVIPSRKNSKYPRKIDKQLYKERFLIEAFFSKL
                     KHFRRIFSRFDKTTSAFSGFLHLAGALIWMR"
     gene            8524..8751
                     /locus_tag="ACF2SP_001710"
     CDS             8524..8751
                     /locus_tag="ACF2SP_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359496.1"
                     /translation="MNKLLRCKTPALDDKQGYLVMKNAGTMDLFELLEALDAGIITLN
                     VKEKLQLTYTIIKAVIEQSHNLNLIHTDLKP"
     gene            8759..8995
                     /locus_tag="ACF2SP_001711"
     CDS             8759..8995
                     /locus_tag="ACF2SP_001711"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359497.1"
                     /translation="MVDPETMNIILIDYAFATPVDQPRTDKSIHGTKEYLAPEIMCDN
                     SITIKSDSFALGLTIAQIWGYLFNLNITPFTSFD"
     gene            9044..9352
                     /locus_tag="ACF2SP_001712"
     CDS             9044..9352
                     /locus_tag="ACF2SP_001712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359498.1"
                     /translation="MPLSALTKDKITKILDALTEFDPAKRIMPADALIQWEHLLNEFD
                     QIEACHDDHQDSVKPPQLKQSLFTRSPAFFSLASCSEDTSDEAEQSEPAPPIWRRDSI
                     "
     gene            complement(9613..11052)
                     /locus_tag="ACF2SP_001713"
     CDS             complement(9613..11052)
                     /locus_tag="ACF2SP_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFD2359499.1"
                     /translation="MKKLIILGCVIFMVTSCSFAPKYERPLMPVPEHFKETGKWVPAK
                     TVIPSSKITPWWEVYNDPVLNKLESLIEVSNQDLKKIFYRYQEALALVQVSRAGFFPS
                     IMGTLNANRQQTSTTMADPSTIPLYNNYIIGSQVNYEIDAWGSIRNAVAAQQNTAHAT
                     EAELAAISLSLHAELASDYFSLRGCDESQRILDATVAAYKKALYLNKKRYHGGASPIE
                     DVDQAETQLENAKTAAADMRLKRAQFEHAIAILIGQFPADFSLAPAKLSRTLVTVAPD
                     LPSTLLERRPDIAAAEFRVQAANAAIGVARAAFFPVLDLTGIIGFQSQSLSNLISKPS
                     LIWSLGPISTLALAQPLVAQPIFEGGKLVGQLKYAKASYFETVANYRQTVLTAFQEVE
                     DSLVAVKQLDKEIRSQRAATQAAKRALTQANNRYSGGIVTFLDVVVVENTALQTELAA
                     VNVFTRRQIASVQLIKALGGGWVYLNKER"
     gene            complement(11049..12194)
                     /locus_tag="ACF2SP_001714"
     CDS             complement(11049..12194)
                     /locus_tag="ACF2SP_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2359500.1"
                     /translation="MLKGIKFQIKEHKRNPLFVFLVLFLLFFLTVIFYRVYSAIILRQ
                     QTRADEVNYVRVLTATPLKGTERIILPGNVQAWHDAVIYARTNGYIKNWYVDIGSHVK
                     KGDLLAVIETPELDAQLRQAEADLNTAIANNQIAQSTAKRWVNLVKSNSVSKQERDEK
                     VSDAASKYALEVAARANVDRLKELVSFERVIAPFDGVITSRTTDIGSLINAGSSDTGL
                     PLFQIAQTNPLRIYVKIPQNYSSRITPDMKVSLTFAEHPGQTFPAQLIKTAQAIDPAT
                     RTLLAQFKAENNNEELLAGGYTEVTFTFTIPKNTFRLPVNTLLFQAQGLQIAVVDKNN
                     QVVLKPITISRDFGNVVEIDQGINAGEKIILNPSDSIINGELVKVVS"
     gene            complement(12187..15363)
                     /locus_tag="ACF2SP_001715"
     CDS             complement(12187..15363)
                     /locus_tag="ACF2SP_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2359501.1"
                     /translation="MWIVRIALARPYTFIVLALLLLIIGPLSILRTPTDIFPDINIPV
                     ISVIWSYSELPPDDMGNRITSVFERVLPTTVNDIEHIESESLIGVSVVKLFFQPGVNI
                     ELALSQVTSIAQTILRSLPEGTLPPLILNYKASTVPILQLVLSSDTIPEQELNDLGNN
                     FIRPQLAPVAGASLPYPYGGKVRQVQVDLDLQAMQTYGVSPLEINTAISAQNLIIPAG
                     TQKIGDYEYIVKLNSSPLKVNDLNDMPVKTTPGRVLYIRDVAHVRDGFTPQTNIVRVD
                     GKRAVMMSVQKTGGASTLGIIKQIKALLPKIKEIMPAGLNLGQFADQSIFVTAAIQGV
                     ITEGIIAASLTALMILIFLGSVRSTLIISISIPLSIIGSLTILSALGETINIMTLGGL
                     ALAVGILVDDATVAIENINWNLEQGKEVEEAILDGAHQIAIPALVSTLCITIVFVPMF
                     YLGGVAQYLFVPLAEAVIFAMLISYLLSRTLIPTLAKYLLHKHDPEIKEQSQNFFVRL
                     HHQFEKKFEIFRERYSNLLNKALHNATYFVGIFLVFVISSVVLLWPWLGSNFFPNVDA
                     GQIKLHIRAPTGTRVEVTARYVDTIDKLIRTIIPPQELDTIVDNIGLPVSGINLSYSN
                     SAPVGPEDADILISLKEKHHSVFDYQRELRTVLNQKFPDISFAFLPADIVNQTINFGL
                     PSPIDIQIIGLKQDENRVYAKKLMQRLKKVPGLVDIREHQAMNYPELFVDIDRSKAKE
                     LGITQRNIAMSLFIALSGSFQTSPTFWLSPENNVSYPLVVQAPQYVMNSLQALRNIMI
                     DGISPNIQSPAQHQILGALGTITRKWTSVVESHYNVMPVIDIFAATQDRDLGSITHDI
                     NQIIAEMNSEVPLGSTIAIRGQIDTQHQAFQGLYWGIVFSVLLIYLLIVINFQSWVDP
                     FIIITALPAAIAGIAWFLFITCTTLSVPALTGAIMCMGVATSNSILIISFARQHLLET
                     NNPLLAAMEAGRTRIRPVLMTASAMIIGMMPMALGLGDGGEQNAPLGRAVIGGLSFAT
                     IATLFFVPTVFYIIHNHQLKVKQRNNHA"
     gene            complement(15489..>15803)
                     /locus_tag="ACF2SP_001716"
     CDS             complement(15489..>15803)
                     /locus_tag="ACF2SP_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359502.1"
                     /translation="ERISVEVFLSLLGINYSKVDVISIEEQNNPVDVQYKECRFQIKE
                     IPDDVKRHRDIKQTYEALEKADKDKIDWPISVYDKSPYQTAWERVTNKLGSVNEIFSA
                     SK"
BASE COUNT         4829 a         3369 c         3015 g         4590 t
ORIGIN      
        1 tatcttcttt ggaaaataaa attaataaag cagtagttgg ctctgaaatg gatagcaatc
       61 aataagtaga taaattaagt attcctcggc aaaccatcct atactattga tattaatagc
      121 aaacaattaa aaggaattag ctatgcgacg tcaatggtat atcggatttg gagttatcct
      181 tttatgtctc tttgccctgt ttgccatgct gcaggatatt gtctggtttt ttgccttttt
      241 gatacctatt gtaatcctgt ggatttatga tgtattgcaa acaaaacaca ccattttacg
      301 caattttcca gtactaggtc acgttagata ttttttggaa tttcttcgcc ctgagattca
      361 acaatatttc atcgctaccg atgaaagcga gcttcctttt aacagggaaa cccgctccat
      421 gatttatcaa cgcgcaaaaa atgtcagaga caccattcct ttcggaacgg agcgagacat
      481 tctaagcgtt ggttatactt gggcactcca ttctttatcg ccgaaacact ctagcgaagt
      541 ggaaccaaga atcgatgtgg gtgggcctga ttgtaaaaaa acttataatg catcccgatt
      601 gaacatatca gccatgagtt ttggctcttt atcctctaat gccataatgg cattaaacaa
      661 gggagcaaaa cttggaggat tttaccacaa tacaggggaa ggggggttaa gcccttacca
      721 tttgcaaggc ggtgacattg tttttcaaat tggtacagct tattttggat gccgtgatga
      781 caaaggcgac tttgacgaaa atgaatttat caaagaagca tcccgtgatg aagtaaaaat
      841 gatagaaatt aaattatcac aaggcgcgaa gccatctcat ggtggaattt tacccgcagc
      901 caaattaaca gaagaaatcg caaaagtaag aaaagtgccc atgggcaaag atgttttatc
      961 gccaattgcc cataccgctt ttgataatcc cgttggttta cttcattttg taaaacgatt
     1021 acgtgatctt tccggcggaa aacccatagg ctttaaactc tgccttgggc gccgacatga
     1081 atttatggct atttgcaaag ccatgctgaa aacaaatatt ttacctgatt ttattacaat
     1141 agatggtgcc gaaggaggga caggagcagc accggttgaa tacaccaatt ttataggaac
     1201 tcctttggaa gccggtctgg tttttgttca taatgccctt gttggcacag gtgttcgtga
     1261 taaaattcgc gtaatttgca gtggaaaagt aaccaacggg tttgatttgc tcactaatat
     1321 tgcccttggg gccgatatct gtaattcagc aagagccatg atgatgtcaa taggatgtat
     1381 acaatcaaaa caatgcaatg cgaatacctg cccgacagga gttgccactc aaaagaaacg
     1441 tcttcagtat ggtcttgttg ttgatgaaaa aaaatttctt gttgctaatt tccataataa
     1501 tacgataaaa agctttttgg aaatggttgg cgcattggga ttaaacaatc ccagtgattt
     1561 aaaaccatcc catattatga gacgaatcgg ggtggatgaa gtcaaatctt ttgatcaaat
     1621 ctatacttac gttaagccag gccaattttt acaaggcgat attcctgatg attatagagt
     1681 ccactgggag catgccgatc cggacaagtt ttaatatcag tgtggccata gcgtgtcggc
     1741 gttgtgattc gtattccatg tgaatcaaag cgggaaaacg aatggtaagt gatttaaagc
     1801 cagattcatt tctttagcaa tgatgtaaat tcagccacat tgaccgctcg agaaaaatat
     1861 aatccctgac catacaagca gcctaagtct cttaattttt ccaatgcatt ttgatcagcg
     1921 atgccatctg ccaggggttc ccaccctaat gtttgggcta gttttactat agcctctaca
     1981 attttggctt tctttgcgtc ttttgcaatt ttcagtacga atgatttctc aattttaatg
     2041 ttttcgatag ggaaattagt caaatgaatg aaggaagagt tctcactgca aaaatcatca
     2101 atgcataatt tgatgcccat ggttgataat cgatcaaaca cttctttggt aaccatctgc
     2161 tcagttaaac aagccctttc ggtaaattca agtttcaaga attgcggggc aatctcataa
     2221 tcatttaata atttctctat taattcaggc aactcatggt gcactgacag atttacagag
     2281 acaaaaattt tatgtccagc atcatgccag gcggctaatt gctttataac ttccttaagc
     2341 ataagagttg tcaaattatg aattaaattg gttccctcga ttaaaggcat gaatttttct
     2401 gcacttaata aacccaattc aggatgttca aaacgagctg atgactcggc acctacaatt
     2461 tggccattag ccagctcaac tattggctgg taataaattt tcaaatcttt gttttcaatg
     2521 gagcgttgca gttcattcat caccatccga ttctgtataa ggtcctcttc catttcgggg
     2581 ctgtaaattg cataggattt gttttccttt cgtgcctggt aaactgcgat aatggcatga
     2641 tttactaaaa catcgtcttc ctctccatgg acaggataga tagcggcacc aacagtagat
     2701 gtcacattga tactaattcc atccaccatc aggttattat tcagcaaggt aagaagatgg
     2761 attaatagct cattaatatc caaatctttt ttaagtctcg gcatcaatat ggcaaattca
     2821 tcactttgca atcttgcaac catattaatt ccaagagaag cttttagcat gtagggatca
     2881 atcaaaatcg attttaattt ttcagcaaat tgctttacga tattattggc attaaatgag
     2941 ctgaaattat aattaagatc gttaattttt atcacaataa cagctaattc accctgtgct
     3001 cccaattgtt cgatggcgag ccgtattttt tcatttaaca agcggttgtt aggcaaattg
     3061 gtcaagctat cgtgattact ttcatattta agctttaatt ccagatttcc actgatattt
     3121 aaaagctctt tggttttatc aaccagtaaa ctctccgctt tgttaaccaa tccataacaa
     3181 aatgcttgcc cccaatacac aaacataaaa ggtgttaaat ccaggatcca aattgccggg
     3241 ttagatcttt gagcctgaat aaacccgcct aaactcacag atccagtcaa ttgataggaa
     3301 acgatacaac ttgcaatcaa gatactgcca acagaaatca gcaatcctaa aaaggcatat
     3361 cgattaacat gacctttcag gataacagag ctatgtgcca aattagattt aatattcatg
     3421 gattaagagt aattccctta tgcctgtcaa caggctaatt tttaattccc catcccttct
     3481 acagcgactt caatgctcta caaaaaattg aataaatgga ccacttaatg attagtatag
     3541 cctaaagaaa aaacaacaaa tgctctatcg tatggagttt ttctggatgt aaaaaccagg
     3601 ttaatgttat aatctaatta acccgatggc gagtgatttt agaaccaaca ctcacagcca
     3661 gggaagcatc ctgttatcgg cgttgagcaa gcccaccaat agagtgggaa gcctgaaccg
     3721 atgcattcgc tgcccacttg aacctaaggg ttcaaaatcc ggtacccatc gggttatatc
     3781 ttcatgagct gctcttttaa tagcaggtat tcatcccgaa ttttagctgc caattcaaat
     3841 tccatatttt gagcatgagc atacatttgt ttttccagtg cattaatctc ttttgccaat
     3901 tcctgaggag accaatgggt atatcgggga gcttgttcag caaccattgt ttttcgttta
     3961 ccgatatagg caccctccaa aatatcctca actgatttat taataccctt gggagtaatg
     4021 ccatgtttca aattgaatgc tttttgtttt tcacgtcgtc tctccgtttc cgtcaatgct
     4081 ctttgcatcg aaccagtcat ggtatcggca tacaaaatag ctcgcccctt cacattacgc
     4141 gctgcacggc caatcgtttg aattaaagaa cgctccgatc gcaaaaaacc ctccttgtca
     4201 gcgtccaaaa tcgcaacaag agcaacctcg ggcatatcaa gaccttcacg caataaatta
     4261 atccctacca gaacatcaaa ctcacccaaa cgcaaatcac gaataatttc catgcgctca
     4321 accgtatcga cgtcggaatg caaatagcgc accttgatcc cgtgctcact cagatactct
     4381 gtcaaatcct ctgccatgcg tttggttaat gtcgtgacaa gaatacggct gccctgagcg
     4441 atcacctgtc tgatttcaga cataagatca tccacctgag ttttcaccgg cctgatttcc
     4501 acctcaggat caatcaatcc ggtcggtctt accacctgtt cagccacatt atccgaatgt
     4561 tcctgttcat aaggtcctgg agttgctgaa atataaatag tctgcggcga acgctcttca
     4621 aattcttcaa aacgcaaagg cctattatcc aaggcagaag gcaagcgaaa tccataatta
     4681 accaaggttt ccttgcgcgc cctgtctccg cgatacattc cacctatttg aggaacagta
     4741 acatgcgatt catcgataat tagcaaggcc tccggcggga ggtaatcaaa aagagtaggc
     4801 ggtgcttctc cggcttcgcg gttggataaa taacgcgagt aattttcaat gccagagcaa
     4861 taacccaact ccaacatcat ttcaatatca aaacaagtcc tttgttccaa acgctgagct
     4921 tctactaact tattttgagc attcaactca gccagtcttt cttgtagttc tacttttact
     4981 ttttctactg tttctaaaat acgttctctg ggagtgacat aatgagtttt aggaaaaata
     5041 gtgacacggg gcaaccgctg taaaatttct cctgttaaag gatcaaagcg ggcaatatta
     5101 tcaacctcat cgtcaaaaag ctcaattctt atcgcttctt tctcggaatc agccgggaaa
     5161 atatcaatga catcaccatg tacccggaat tgaccgcgct caaggctgag attggttcgt
     5221 gtgtactgca tttctgcaag acgctttaaa attttacgct gatcggattg ttctccacgg
     5281 gagagatgta ataacatacg cagataggaa tccgggtcgc ccaatccata aatggcagaa
     5341 accgtcgcta caataatggc atctttgcgt tcaatcaacg ctttggttgc cgacaagcgc
     5401 atctgctcta tatgttcatt aatggaggca tccttttcta taaaagtatc tgaagcaggc
     5461 acatacgctt cgggctgata ataatcgtag taggaaacga aatattctac tgcattatca
     5521 ggaaagaagg ctttaaactc tccataaagt tgagccgcaa gcgttttgtt cggcgccatg
     5581 atcaaagtgg gtcttttcat ggcttgaata acatgagcta tagtaaatgt tttaccagaa
     5641 ccagtaacgc ctaataatgt ttgtttggct aaacctgatt caagaccatc aatcaaagag
     5701 gctatggctg taggttgatc gccggcaggt tggtaatttg agtaaatttt aaataagtct
     5761 ttcatatttt atattatatg ctagtacccc tataattcac aggagcaaca gatggatatc
     5821 gcattggcaa agcgtgtaca aaaagtaaaa ccttcaccta cccttgccgt agctgcaaaa
     5881 gcagcgcaga tgaaagccca ggggcttgat atcattggcc ttggaactgg tgaacctgat
     5941 tttgacacgc cgcagcatat aaagctagcc gccatctctg ctattgaagc aggtgatacc
     6001 aaatacaccg ccgttgatgg gatagtcgaa ctaaaggaag cggttaaaaa caaattcaaa
     6061 agagacaatg aactggatta tcaactcaat cagatattgg tctccgtagg gggtaaacaa
     6121 agctgctata acctctgcca agcttacctt aaccctggcg atgaagtcat catcccagct
     6181 ccttattggg tatcctatcc tgacatggtt ttgctggccg atggcgttcc tgtcatcatt
     6241 gaaacgactc ccgcgcagcg ttataaaatc aatgcacaac aactggagca agccatcact
     6301 ccaaaaacgc gaatgatttt cctgaacagc ccttccaacc cctctggaat agcctacacc
     6361 cagaacgagt taaaggaatt aggcgacgtt ttgaaaaaac atccacagat acttattgct
     6421 actgatgaca tgtatgaaca tattatctgg agtcaagctt ttacaaatat actcaatgcc
     6481 tgccctgaat tatatgacag aacgatcgta ttaaatggcg tgtccaaagc ttatgccatg
     6541 acaggctggc gaataggcta tgcggcaggc cctgcgccat taatcaatgc gatgaaaacc
     6601 attcaatccc aatccacatc caatccatgc tctatagcac aaagagctgc cgtagcagct
     6661 ttgaatggca gcaatgaaag cattgaagaa atggtcaacg ccttccatca aaggcacgac
     6721 tacgtggccg atagattgca aagcatcgat ggcatagagg tcatccccgc cgacggaact
     6781 ttctacattt ttcccagtgt gcaggcgatt atcgaaaaac gaggttacgc caatgacatc
     6841 gaattttccg aaaaattgct gaatgaagta ggtgtggcac tggttccagg ctccgctttc
     6901 ggcacagaag gctgcatcag aatatccttc gccaccggta ttgacaccct gaaagatgcc
     6961 ttgaacagat tacaacgttt ctgcagttaa aaaatatatt aaaaacctct tgattctctc
     7021 gcggaatcgt tttaagattc cgcctcaatt ccccggtagc tcagtcggta gagcggatga
     7081 ctgttaatca ttaggtcaca agttcgagtc ttgtccgggg agccaatccc agtctgggtt
     7141 ttggcgaaat tcaaataagc cttaaatttt acgtgtcgaa tatgtgtcga agtttgaggt
     7201 aaagcattta accgactctt tcccatttaa ccttaatctg taattttcaa tattcctaaa
     7261 tccataagaa agatgttgaa tcagctttat ttttgatatt cctaacttta tataagcttt
     7321 gcctatctgg ggggaatttt cttacgccca ggagagattt taaagccctc tggaactggt
     7381 ttaaatggat ctttatctct aggccaactt ttatcgcgag actgaaatgt cctaagagca
     7441 tttatatcta actcgactga aactaagaaa tcattttttc cacctctaag ccttactaaa
     7501 tcccttttga attcttgctt actaggttct gtgaacgaaa tttttagcgc atccaaatga
     7561 gcgctccggc caaatgcaaa aacccagaaa aagcggaagt ggttttatca aaacgagaaa
     7621 aaattctgcg gaaatgcttc aatttagaaa aaaaagcctc gatcagaaaa cgctctttat
     7681 aaagttgttt gtctattttt ctggggtatt tagagttttt tcttgatgga atgactggag
     7741 agcagcgctg atggtgtagc gtatcaatga gttcttgaga gtcatatccc ttatcagcaa
     7801 ggactattgt gtcgtgttgt tccttgagta gctctgctgc ttgagtaata tcattgcggt
     7861 gtcctcctgt cagaataaat ttaatcggat tgcccaaccc atctaccaaa gcgtgaattt
     7921 tagtggtaaa accacctttg ctacgcccca atgcttgagt ctcatttcca tccttgacat
     7981 aacccgaggc gcaagcgtga gcacgtacaa tagttccatc gatcatcatc gtttggatat
     8041 caacatcact gacaactgac attaactcag accagatgcc acggtcagac cacactttat
     8101 aacgtcgatg aatctgacgc caatgaccat aatagtacgg aagcagtcgc cattggcatc
     8161 cggtacgtaa tacataccaa actccttcaa tgaagcggcg taaactcgct tcatttttga
     8221 catgcagacc cttcaggcca cgcaggtaag aatagatttt actccacaat tcttctgata
     8281 tgttatgatg cattctggca gttccattag tgttttttac gatcgtcaat cgaattccac
     8341 tataaaactg ccttcttttc aattaattaa aaatttcgtt cacagaacct aggagtactt
     8401 gcccctgaag aaaattattt atttaaaagc aggacagacg aaaaagccag aatatgtaag
     8461 attattccca ttaccaaggt ctttacccag gaagtgattg tccaagaggg agcgaatacc
     8521 aaaatgaata aactcctgcg ttgtaaaaca ccagctttgg acgataaaca aggttacctg
     8581 gtaatgaaaa atgcaggaac aatggatctt tttgagctct tggaagcttt agacgctggt
     8641 attattacac ttaatgtaaa agaaaaatta caattaactt acactattat aaaagcggtg
     8701 atagagcaaa gtcataattt aaatcttatt catactgact taaaaccatg aaaacttcat
     8761 ggtagatcct gaaacgatga atattatcct cattgattat gccttcgcaa ccccagtgga
     8821 ccagcccaga acggacaaat caatccatgg gacaaaggaa tatcttgccc ccgaaatcat
     8881 gtgtgacaat tccataacca tcaaatcaga ctcttttgct ctggggctca ctattgcgca
     8941 aatttggggg tacttattca atttgaacat tacaccattt acaagttttg attagctcta
     9001 tgagtttcat tcaacccgaa catggacgca cttatttgag aagatgcctt taagtgcatt
     9061 aaccaaagac aaaataacca aaattttaga tgctttaaca gaatttgatc ctgcaaaacg
     9121 aataatgcca gccgatgcgc tgattcaatg ggaacacctg ctaaatgaat ttgatcagat
     9181 tgaggcgtgt cacgacgacc accaagactc tgtcaaacca ccacaattga aacaatcgct
     9241 gttcacgaga agtccggctt ttttttcact agcctcctgt tctgaagata ccagcgacga
     9301 ggcagagcaa tcagaacctg ctcccccaat atggagaaga gacagtatct agatcatcaa
     9361 gtgatttaag tttatataca ccgcatagtc tcatcgtacc tcggattatc tgcagactcc
     9421 agggtcagag aaaatccgtg gggcattggc acaatgtgct cacgattcaa ggacaagcgt
     9481 agcaaggtca agacttttta ttagtcaggc aacatccctg aagtttgttt agacacgctt
     9541 cagggcattg cctaatattt tggcataata agcatcaaat tctattgata catatctgaa
     9601 ttattgggtg ctctacctct ctttattcaa atacacccat ccaccgccta atgccttaat
     9661 taattgaaca ctggcaatct ggcgtcgagt aaacacatta acggctgcga gttctgtttg
     9721 taaggcagta ttttcaacca ccacaacatc gagaaacgtg actatccccc cagaataacg
     9781 attgtttgct tgtgttaagg cacgttttgc tgcctgtgta gccgctcttt gactacgaat
     9841 ttccttatcc agttgtttta cagcaaccag gctatcttct acttcctgga acgccgttaa
     9901 taccgtttgt ctatagtttg ctaccgtttc aaaataactg gccttggcat attttaactg
     9961 ccctactaac ttgcctcctt caaaaattgg ctgagcaacg agaggttgag ccaaagccaa
    10021 ggtgctgatt ggccctaaag accagattag actgggcttt gaaatcagat tggataagct
    10081 ctggctctga aaaccaataa tcccggtcaa atccagtacc gggaaaaatg ccgctcgagc
    10141 taccccaatt gcagcattag ctgcttgtac acgaaactct gctgctgcaa tatccggtct
    10201 tctctctaat aaagtcgatg gaagatctgg agcaacggtg acaagcgtcc gtgataattt
    10261 agccggagct aaagaaaaat ctgctggaaa ttggccaata agtatcgcta tagcatgttc
    10321 aaactgtgct cgtttcaggc gcatatctgc tgcagcggtc tttgcatttt ccagttgcgt
    10381 ctccgcctga tctacatctt ctattggaga agcgccacca tgataacgct ttttatttaa
    10441 atacaaggct tttttataag cagcaaccgt agcatccaaa atacgttgtg attcatcaca
    10501 acctcttaaa gaaaaataat cactagccag ttccgcatgc aaacttaaag agatagctgc
    10561 aagttcagct tcagttgcat gtgctgtatt ttgttgtgcc gcaactgcat tacgaatact
    10621 tccccaagca tcgatctcat aattaacctg agaaccaatg atgtaattat tatacaaagg
    10681 aattgtcgat ggatctgcca tggtagttga tgtctgttgc ctgttggcat taagagttcc
    10741 cataatgctg ggaaaaaaac cagctctgga tacctgaact aatgcaagcg cttcttgata
    10801 gcgataaaat atttttttaa gatcctggtt tgatacctcg attaaactct ctaatttatt
    10861 taaaaccgga tcgttataga cctcccacca gggagtaatt ttagaagaag gaatcacggt
    10921 tttggcagga acccatttcc cagtttcttt gaaatgctca ggaactggca tcaatgggcg
    10981 ttcgtatttg ggagcaaacg agcaactggt taccataaaa ataacacatc ccaaaataat
    11041 gagctttttc atgaaacaac cttaacgagt tcaccattta ttatagaatc agagggatta
    11101 agaataattt tttctcctgc gtttattccc tgatcaattt ctactacatt gccaaaatca
    11161 cgactgattg ttattggctt taaaacgacc tggttatttt tatcaactac agcaatttgc
    11221 aatccttgtg cctgaaacaa aagcgtattt accggcaaac gaaatgtatt tttaggaatg
    11281 gtgaatgtaa aagtgacttc cgtatatccg cctgcaagca gttcttcatt attattttcc
    11341 gctttaaact gagccagtaa ggttcgagtt gctggatcaa tagcctgcgc ggttttaatt
    11401 aattgtgcag gaaaggtttg acccgggtgc tctgcaaaag tcaagctgac cttcatatca
    11461 ggagtaattc gtgaggaata attctgagga attttaacgt aaattctcag cggattggtt
    11521 tgagctatct ggaacaaagg taaaccggta tcactacttc ctgcattgat caatgaacct
    11581 atatctgtag tcctggatgt aattacaccg tcaaaaggag caataactct ctcgaaactc
    11641 accagttcct tcaatcgatc tacattagca cgtgctgcta cttcaagagc atatttcgag
    11701 gcagcgtcgc ttactttttc atcgcgctcc tgtttggata ctgaattgga ttttaccaga
    11761 ttcacccaac gtttagcggt agattgagca atttgattat tcgcaatagc agtatttaaa
    11821 tctgcttctg cctgacgaag ttgagcatcc agttcagggg tttcaattac tgcaagtaaa
    11881 tccccctttt ttacatgact gccaatatca acataccaat tcttaatgta tccattcgtt
    11941 cgcgcgtaaa taacagcatc atgccaagct tgcacattac ccggcaaaat aattcgttct
    12001 gttcccttaa gaggagtagc agtcagcact ctgacataat tgacctcatc ggctctggtt
    12061 tgttggcgta agataatcgc tgaataaaca cgataaaaaa taaccgttaa aaaaaacagt
    12121 aaaaaaagaa ccagaaacac aaacagcgga tttcttttat gctctttaat ttgaaattta
    12181 attcccttaa gcatgattat tcctttgctt cactttcaat tgatgattat gaatgatgta
    12241 aaaaaccgta ggcacaaaaa agagagttgc tattgtcgca aatgacaatc ctccaattac
    12301 agctctgcct aatggcgcat tctgctcacc gccatcgcct aatcccagag ccatgggcat
    12361 cataccgata atcattgctg aagcggtcat caatacaggt cgaatacgag ttcgccctgc
    12421 ctccatagcg gccagtaaag gattgtttgt ttctaacaaa tgctgacggg caaaactgat
    12481 aatgagaata ctgtttgatg tggcaactcc catacacata atcgctcctg tcaatgcagg
    12541 aacactcaaa gttgtacagg taataaataa aaaccaggca atgccagcaa ttgcagcggg
    12601 taaggccgta atgatgatga agggatcaac ccaggattga aaattaatca ctataagtaa
    12661 ataaattaat agaacagaaa aaacaattcc ccaatacagt ccctgaaatg cttggtgctg
    12721 ggtatctatc tgtcccctga tcgctatggt tgaacctaaa ggcacttcac tgttcatctc
    12781 ggcaataatc tgattgatgt catgagtaat cgagcccaaa tctctatctt gtgtggctgc
    12841 aaaaatatca atgaccggca taacgttata atgagattca actacagagg tccattttcg
    12901 tgtaatggtg cccaatgccc ctaatatttg atgttgtgct ggtgattgta tgttgggaga
    12961 aatcccgtca atcataatat tgcgtaatgc ctgtaatgaa ttcatcacgt attgtggagc
    13021 ctggactact aaaggataag aaacattatt ttcaggactt aaccaaaatg taggagacgt
    13081 ttgaaaactg cccgacaatg caataaataa actcatggca atgttacgtt gtgtaatccc
    13141 cagttccttt gccttgcttc tatcaatatc aacaaatagc tcaggataat tcatggcctg
    13201 gtgctctcga atatccacaa gaccaggaac tttttttaat cgttgcatta actttttcgc
    13261 atacactctg ttttcatctt gcttcaagcc aatgatctgt atatcaatgg gtgaaggtag
    13321 accaaaatta atggtctgat tcacaatatc tgcgggtaaa aaagcaaagg atatatcagg
    13381 aaatttctga ttaagcacgg ttcttaattc acgttgatag tcaaaaacag aatgatgttt
    13441 ttctttcaga gatatcagga tatccgcatc ttcaggtcca acaggagcag aattactgta
    13501 cgataaatta attccgctca ccggcaaacc tatattatcc actatggtat ccagctcctg
    13561 tggaggaata atggtcctaa ttagcttatc tatagtgtca acataacgtg ctgtaacctc
    13621 aactcgggtt cctgtcggag cacgtatatg aagctttatc tggccagcat caacatttgg
    13681 aaagaaatta ctacctaacc agggccataa taaaacaacg gatgaaataa caaatactaa
    13741 gaaaattcca acaaaataag tggcattgtg taaagcctta ttcaataaat tactgtatct
    13801 ttctcgaaaa atctcaaact ttttttcaaa ttgatgatga agtcgtacga agaaattctg
    13861 agattgttct ttaatttcag gatcatgctt gtgcagtaaa tattttgcca atgtaggtat
    13921 gagcgtccgt gataaaagat aagagatgag catggcaaaa atcaccgctt ctgccaaagg
    13981 cacaaataag tattgagcga cccctcctaa ataaaacatg ggcacaaaaa caatggtaat
    14041 acataaagta gacactaagg ctggtatggc aatttgatgc gctccatcta aaatagcctc
    14101 ttctacttct ttaccttgct ccaaattcca attgatattt tcaatagcaa ccgttgcatc
    14161 atcaactaaa atacctaccg ccaatgccag accacccaat gtcataatat ttatcgtttc
    14221 gcccaatgct gaaagaatgg tcaatgaacc gatgatagat aaaggaatgg atatgctgat
    14281 gattagagtg ctgcgaacac tgccaagaaa tattagaatc atcaaagcag tcagagatgc
    14341 agcaataatc ccctcggtaa tcactccttg aatcgctgcg gtaacaaata tagactgatc
    14401 cgcaaattgt ccaaggttta aaccggcagg catgatttct ttaattttgg gcaataaggc
    14461 ttttatctgc ttgataattc ccaaggttga agcaccaccc gttttttgaa ctgacatcat
    14521 tacagcgcgc ttaccatcga ctcgtactat attggtttgc ggcgtaaaac catcccgtac
    14581 atgagcaaca tcacgaatat ataaaacacg accgggagtc gttttaacgg gcatatcatt
    14641 taaatcgttt accttaagag gactgctatt gagctttaca atgtactcat aatcgccaat
    14701 tttttgagtt cccgcaggaa tgattagatt ttgtgctgaa atagccgtat taatttctaa
    14761 aggagatacg ccataggttt gcatggcttg caaatccaga tcgacttgta cctggcgtac
    14821 tttgccacca tagggataag gtaaggaagc cccggccaca ggagctaatt gtggacgaat
    14881 aaagttatta cccagatcat taagttcttg ctccggaata gtgtcactgg ataaaaccag
    14941 ctgcaaaata ggaacggttg atgctttata attcagtatc aatggaggca aagttccctc
    15001 aggcaaggaa cgcaaaatcg tttgagcgat agaagtaacc tgactcaggg ccagctcaat
    15061 attcacaccc ggttgaaaga atagcttgac tacgctgacg ccaattaatg attctgattc
    15121 aatatgttca atatcattga ctgtagtggg taataccctt tcgaaaacgc tggtaattcg
    15181 attgcccata tcatcaggcg gcaattctga gtaactccag atgacactga ttaccggaat
    15241 gttaatgtcc ggaaaaatat cggtgggagt acgcaggata gacaaaggac caatgatgag
    15301 aagcaataga gctaatacaa taaatgtgta aggtcgtgcc agcgcaatcc gtacaatcca
    15361 cataacgttc catgtataaa atcgagataa tatctcagtt taatgggata tcagggaaat
    15421 aaaaacagtt catataaaaa atcctaataa aactattttt tctgggtttt tgcatttggc
    15481 cggggcgctc atttggatgc gctaaaaatt tcgttcacag aacctagttt atttgtaact
    15541 ctttcccatg ctgtttgata aggagactta tcataaacag atatcggcca gtcaattttg
    15601 tctttgtcag ctttttctaa tgcctcataa gtttgtttta tatctctatg tcttttgaca
    15661 tcatccggga tttctttaat ttggaaccta cactctttat attgaacatc aacaggatta
    15721 ttttgttcct caattgaaat cacatctacc tttgaataat ttatacctag aaggctgaga
    15781 aacacctcaa cactgattcg ctc
//
LOCUS       DAXLDT010000068        15396 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXLDT010000068 DAXLDT010000000
VERSION     DAXLDT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.068"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..209)
                     /locus_tag="ACF2SP_001717"
     CDS             complement(<1..209)
                     /locus_tag="ACF2SP_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFD2359503.1"
                     /translation="MGSLRKHQPGKTTDLLMSPFLRLQQEVNHAMSDFYNLFDPQRSA
                     NLEQFEHISLTPSLDIVEDKDNFKI"
     gene            complement(471..1457)
                     /locus_tag="ACF2SP_001718"
     CDS             complement(471..1457)
                     /locus_tag="ACF2SP_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFD2359504.1"
                     /translation="MHAMIMEKPGCALQYQEVNKPNPNENELLIKVSACGICRTDLHV
                     VDGELKNPKTPLIPGHQIVGTVEQVGSAVKKFAIGQRVGVPWLGGSCGICNFCTSGRE
                     NLCDQACFTGYQIDGGFAEFCVANYHFCFPIPDGYPDHQAAPLFCAGLIGYRALLKAG
                     NATHLGLYGFGAAAHILIQVACQKGIKIYAFTSPGDQETQKFAYRLGAVWAGDSTQSP
                     PHLLDAAIIFAPVGALVPQALHNTVKGGTVVCAGIHMSDIPSFPYEILWGERTVCSIA
                     NLTRKDGEEFLSLAPKIPVKTKVQTYPLTEVNRALDDLRHGRYRGAGVIVMK"
     gene            1588..2037
                     /locus_tag="ACF2SP_001719"
     CDS             1588..2037
                     /locus_tag="ACF2SP_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFD2359505.1"
                     /translation="MRIGEFCNRDVVMMNGDESVKQAAELMRTHHVGDIVLVEEFKGH
                     RVPIGIVTDRDLVVEVMALDVNPEGLAVQDIVTRSVLVAREEDSLIDSLEFMKEKGVR
                     RLPVVDNDHELVGIITIDDITELLAEMLHKVVGVVDRQQKIEKQQRS"
     gene            2117..2680
                     /gene="thpR"
                     /locus_tag="ACF2SP_001720"
     CDS             2117..2680
                     /gene="thpR"
                     /locus_tag="ACF2SP_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFD2359506.1"
                     /translation="MNAIRAFFAISLPPAIQENIRSNLEPLRQMAPVHSIRWTKPMHL
                     HITLQFLQRLETKHIQVLIEKVSNELNHIKQFDLEFGVLEWFPSPKNPRLISLSVDPQ
                     DRLRDWSRMIGQVVDSLKYPLEKRPFRGHLTVGRVVNFSPKLQQLLQIKFPIIPPVPI
                     NKFYLIESRPDKEGSNYFPLAQFDLTR"
     gene            complement(2804..6688)
                     /gene="lepB"
                     /locus_tag="ACF2SP_001721"
     CDS             complement(2804..6688)
                     /gene="lepB"
                     /locus_tag="ACF2SP_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS GTPase-activating effector LepB"
                     /protein_id="HFD2359507.1"
                     /translation="MLTYQGKEIVRFKEKTGGKNKSDVDGFYKDSDGRKFFIKKPGDP
                     RELFTELFAGLLLKEFMKRGLIDESYFPSLICADVIQFEDKSYGLIQPLVSFDELHKI
                     IGTSYGDGSDRNTIKETFFGPGYYAGITKQNKYFGLSMALMFSLLLGAHSVHSGNIVV
                     LKGEGEEKSKQFGRIDWGDAFRYFAHRNNNDNLLYAYENRGWFNYKSLTKDYFLNYKK
                     INGLFPAMAEKARQLQSKLNPELLIEIVTGALKNIPADLIEEKTKIQLAAYMCMDSFK
                     EVTFGPKGNCKDFAIAMATLLENRLGKIAVLKDMSPLSNPEELYQSILELKPLTLLLT
                     SSTSFSETINQWADILKTTDMEKFSFDSNPINLMELVKQFNLYVDELAITCEANNVWA
                     KERIDSTPNLFALYDNSGGEAMHGHAFVPYYKESIVLRRLFTVDPNTFNLSRFAAFEG
                     PCQLYCKEHKDSAWVKIQNLLTLGNGIINTLKIIKQAQAFGIDEAVTENLKALKEQFI
                     AFQLAEADVKESLKAPSFAEPSPNKESEFFYPIDEEALAKMNGYQLATICLEELNSPK
                     PSPLIERILSNKKFWKRINSAFESGIFKGRTDDPAGKIAKIREWHQLLQISGKKTAGQ
                     IDELQKIVISLQSKIKKQTIEFEELEATLIQIKEKYQLLEKMAEQSEHEKSSAQSIIR
                     SLNLELGQLKLQLQEQEKLQFQLKELKEKIHEQTTLSKRLGEELQTQKKTNTHQEETI
                     QRISKEKSLADSSLESLRKELHELVKKERSLHKTLEEKQLQVQHLEEQLAEKEKENLT
                     LKKADKQSQHEKSLDKSAIESLTSELNQLKLELQKQETLELQLKSLREQVQEQTLLVE
                     GLKEELQKQKKSNTHQEETIERITKEKSLADSALESLRKEMHELTRKNEENQLKLTKQ
                     ANSLSEQLEEKQLQIREFEKQLQEKEKRVEQSEKEKASAKRTVTSLREQVSNLKLQLQ
                     QLGEVIQEKEKGSSLISQQSKQIIALQEIIEDQKRQLEELKIKIQELVSANQELGKQN
                     QSLSKENLHNKNTVEDLKKKLTELNVQLEQLHQSSSEKEQTIRKLREELIKKDSSLKQ
                     NEEMQLAQKHLQEEIDRLQKEIKQQQLNTNQLESIIAQSKEAEKRYQQALQQKKGIYL
                     ARMERVSPIYLQIQQIEQKAKELEERRETEASTAAKTLAKKLRLEIKNYLDNNESDEK
                     SALNSFKINAKRHIENSKETLNQHREEWKYLLANVTLGVFLLGIGYLAAILINKATTG
                     NYTFFSQTNSGKKLDALEKAISSTHSETLVYG"
     gene            complement(6768..7004)
                     /locus_tag="ACF2SP_001722"
     CDS             complement(6768..7004)
                     /locus_tag="ACF2SP_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359508.1"
                     /translation="MLQPFITDFIKNRSFQFIFVMGLVADIALAGTIKHIVFLFGFPY
                     LSSVGLYCLMKRKKTTVKARISEQYRPKQKEICR"
     gene            complement(7040..7723)
                     /gene="radC"
                     /locus_tag="ACF2SP_001723"
     CDS             complement(7040..7723)
                     /gene="radC"
                     /locus_tag="ACF2SP_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFD2359509.1"
                     /translation="MMVAHTAQQLDLREKLLTNGVHSLSDIELLAIFISSGNNRKSCL
                     QLAYELTKHLGNLRNILNADLQSFKSIHGLGEVRYAQLQAAKEICHRSDFIDLQKEIR
                     LSNTQQTYAFLKKRLRDYKNETFAALFLDNQHRIIAYEELFSGTINTATVYPRPIVER
                     VLQLNAAALILAHNHPSGLSDASQQDLAITERIRDALDLVDARLLDHIVIGDNEVYSI
                     FAENKWVCN"
     gene            7800..8996
                     /gene="coaBC"
                     /locus_tag="ACF2SP_001724"
     CDS             7800..8996
                     /gene="coaBC"
                     /locus_tag="ACF2SP_001724"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFD2359510.1"
                     /translation="MQDFINKKILLGICGGIAAYKSAHLVRELTRLGADVRVVMTHSA
                     QQFINPLLMQALSGNEVRIDLFDTQAERAMGHIELARWADYLLIAPASANCLAKMAQG
                     LADDLLSTLYLVTETPVIVCPAMNRSMWAHPATKANCELLKERGVFFVGPEEGSQACG
                     EHGLGRVSEADQIVNTLRLLDVNQLLHGKRIVITAGPTRESIDPVRYLSNYSSGKMGF
                     AMAEAAAMAGAQVTLISGPCHLQTFSDIELIRVESAQSMFDEVNRHLQQGDLFIGTAA
                     VSDYSVLNPASEKMKKNENSELTLQLVRNTDILSEVAKSGKASFVVGFAAETTDVISY
                     AKEKMKNKKLDMIIANQVGKGMGFESDVNQVTVITKNKQIELPLTHKTRLAGQIIAIL
                     AASLQN"
     gene            9023..9481
                     /gene="dut"
                     /locus_tag="ACF2SP_001725"
     CDS             9023..9481
                     /gene="dut"
                     /locus_tag="ACF2SP_001725"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFD2359511.1"
                     /translation="MHQVIQLKILDSRIGDTIPLPAYATDGSAGLDLRVCISEPMQVA
                     PQQTVLLPTGIAIYIADPKLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKISCW
                     NRSQEHFTVNPGDRIAQLVFIPVVQTSFEVVNEFTESSRGEGGFGSSGRY"
     gene            9482..10870
                     /locus_tag="ACF2SP_001726"
     CDS             9482..10870
                     /locus_tag="ACF2SP_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFD2359512.1"
                     /translation="MTYQQKQIPRSVFRAYDIRGIIGKELDENSFYSIGLAIARYLQK
                     LNRRQIFVARDGRLTSYALATALKEGLLDSGIDILDLGAVATPVMYYATHTQGIDSGL
                     MVTGSHNPADYNGIKLVLAGKTLIQDEIDTLYALLGEVKQSAVRGKETAFDVIEDYIQ
                     RIVSDIQLKCPLKVVVDCGNGVAGPIIPRVISKLGCEVIPLYCEVDGRFPNHHPDPSI
                     EANLADLKVAVANHQADIGLGFDGDADRLGLVTNKGEVIWPDRLMMFYSREILSRNPG
                     ATVVFDVKCSSHLEKEIQAAGGVARMCPTGHSIVKAVMKKEQAILAGEMSGHLFFKDR
                     WYGFDDALYSACRLLEIISSSHLTVSEQFELIPNSVNTPEIKIAITDEEKFDFIERFS
                     EQADFPEGRILNIDGLRVEFPNGWGLLRASNTTPCLVARFEATDKDHLEQIQYLFKKQ
                     IQRLDSELDLPF"
     gene            complement(10976..12709)
                     /locus_tag="ACF2SP_001727"
     CDS             complement(10976..12709)
                     /locus_tag="ACF2SP_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD2359513.1"
                     /translation="MNSEVDTLFAQAYKLQYEGHLPQAISLYEQILAQSPKHTETLHF
                     LGLTYAQLGDMENAILYFLQAKKINPNDVGILNNLANAYKKAGQLDEAIKYYQQAIEI
                     KPEYVQAHNNLAATYALLNNYQKALHHYVIAVNTEPDFSAAHFNLGLLLLKNQQLSAA
                     KTQFNNVIALNPKHREAQFYLGILHLEDNLLSEAEQAFHKVLEQDHEHVQSLINLGVI
                     ALKREQNQLAVDYFTKALALDNEDIDARNNLAATFMHHDRFENALMHYDVLLKKEPNN
                     LEYLYNSGVAQMALGHLNEATLLFDQILTLQSDHTPSLNNLAAIYLKMDMRETAREYL
                     ERALVINPDDVVSRHMLNAITGATSVNTTEKYAQNLFNNYALYYDQHMQGELHYKIPH
                     HIGRLIHQLQLLQTSHSLDLGCGTGLTGIVLREISKHLTGVDIAEKMIARAKEKNIYD
                     LLVCSELIDFLRKNKNDYDLAVAADVLPYFGNLDDIFNYINQHLKQEGYFIFTTEIST
                     TTPWKLESSARFSHQPEYIESLINKYQFHLIKQEKIPGRTQNKQVLEVMLYCVKKSTS
                     RFVSNRTYENP"
     gene            complement(12711..13295)
                     /gene="rdgB"
                     /locus_tag="ACF2SP_001728"
     CDS             complement(12711..13295)
                     /gene="rdgB"
                     /locus_tag="ACF2SP_001728"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFD2359514.1"
                     /translation="MKEIILATSNPGKIKELEQLLAPTICIPQADLGIFDAEETGLSF
                     IENAILKARHASSLANKPALADDSGLVVPSLNGEPGIYSARYAGRKANDEDNIQQLLS
                     KMADLSQEQRQAYFFCAIALMQHAKDPTPIIATGIFHGIISVKPSGTNGFGYDPVFYL
                     NEYQCTAAELPAKIKNRISHRAKALNQLRALLPD"
     gene            13344..13901
                     /locus_tag="ACF2SP_001729"
     CDS             13344..13901
                     /locus_tag="ACF2SP_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFD2359515.1"
                     /translation="MRILEKNIKKTPWYLKPFFWNQKRKYGQILEPALIWARIPRLFL
                     AVAAMYGVIDRKKSPLSPILRSLITVRVSQINWCAFCVDINSATLIKRAGSEAQYKQL
                     AKWRSSSLFSPEEKAALDYAEKVTYSDQRPDDECFNQLKLYYSEQEIIELTALIAFQN
                     MSSKFNSALGVEAQGFCSRQLTGKS"
     gene            complement(13928..15274)
                     /gene="sdbB"
                     /locus_tag="ACF2SP_001730"
     CDS             complement(13928..15274)
                     /gene="sdbB"
                     /locus_tag="ACF2SP_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbB"
                     /protein_id="HFD2359516.1"
                     /translation="MAKTITKDSFFHSRLQQFVANSLFPAGAGDWYKNKGYGKKGEVD
                     DEAPFRDFVEKQKTDKKSHYYKEFQGLDLSLKKVKTKLVSGESCQLEVMKCQPESHEP
                     KKPGTGKHIVYFPGANTYYQACFRDISTACKETGATVHAFNFPGTGLSSGKVREANDL
                     INAGISVVSSLIKQGVHPDDIILQGDCYGASIALEVKKQLEDQADIKVRAIMNNVFKS
                     FKAAVCDMITQSPWLPNILKSIVKRLLEFTGWHITPGKKYKHADPYQCHIQHLGDQTL
                     ESSTLSSKVSKYQHEIQTGQTKSQKRAPITDTCPEEYRKDRDELDRKHYVRVKESAKE
                     RLASKFGVDKFGRVNAHFADLCELEMLDGQSVYQGFVNDYIARSNAYIEKHPQKGIKD
                     IQQDLSQLHYLQAADSIEITEDEAQDFNTVVDLITDEQQLRHDRVNDNTIGKSISM"
BASE COUNT         4314 a         2771 c         3089 g         5222 t
ORIGIN      
        1 tcaattttga aattatcttt atcttcaacg atatccaaag acggagtaag agaaatatgc
       61 tcgaattgct caagattggc acttctttga gggtcaaata aattataaaa atcactcata
      121 gcatgattga cctcttgctg aagtcttaaa aacggactca tcagaagatc agttgtttta
      181 cctggttgat gttttcttaa gctgcccatg atgtcacctc cttattccat aaattgacgt
      241 atcagttagt tgttaaaaac actgaaagta atattcacca gcttatcaat gtaaatatag
      301 ttgctggata aaatattttc aattgttata aatttatttc ataatgattt tatattgttg
      361 tttttgtatt tgttttattc ttttaaatgt tgcttaaacc aattttgagc gaggcgagct
      421 acttccttga tgatttgaat ttgttgtaat agcttttgat aattatagct ttatttcata
      481 acaataaccc cggctcctct atacctgcca tgtcttaaat catccagtgc tcgattaact
      541 tcagtaaggg gataggtttg aacttttgtc ttaactggga ttttaggggc cagagacagg
      601 aactcttctc catctttgcg agttagattg gcaattgaac atacagtgcg ttcaccccac
      661 agtatttcat aaggaaagct gggaatatcc gacatatgta taccagcaca aaccacagta
      721 cctcctttta cagtattgtg aagagcctga ggcactaatg caccaacggg tgcaaaaatg
      781 attgctgcat caagaagatg aggtggtgat tgcgtagaat ctccggccca gacagcacct
      841 aatcggtatg caaatttttg agtttcctga tcacctgggc ttgtaaacgc ataaattttt
      901 attccttttt ggcaagctac ttgaatcagt atatgtgctg ccgctccaaa tccatatagg
      961 cctaaatgag ttgcgttacc agcctttagc aaggcacgat atccaattaa accagcacaa
     1021 aataatggtg ctgcctgatg atccgggtat ccatcaggaa tagggaagca aaaatggtaa
     1081 tttgctacac agaactcagc gaaaccacca tctatttgat acccggtaaa gcaggcttga
     1141 tcacataaat tttctcggcc agaggtgcaa aaattacaga tcccgcaact tccacctagc
     1201 caagggactc caactcgctg tccaattgca aatttcttta cagcggatcc aacctgctca
     1261 acagttccta caatttggtg gcctggaata aggggagtct tgggattttt cagctcacca
     1321 tcgacaacat gcaagtcagt tctacaaatc ccacaagctg atactttaat taaaagttca
     1381 ttttcatttg gatttggttt attgacttcc tgatattgca atgcacatcc aggcttttcc
     1441 atgatcatag catgcatggg tattggtcct gtttttatcc tctatcttac aattatagta
     1501 gggtgggtta tacttactta atgcattcaa aaagtaacaa tatttgacat caatgctgat
     1561 gaatgaatag cttgcctgga ggccattatg cgaattggag agttctgtaa cagggatgtg
     1621 gtaatgatga atggtgatga gtctgtaaag caagctgctg aactgatgcg tactcatcat
     1681 gttggtgata ttgttttagt agaagagttc aaaggtcatc gggttcctat tgggattgtt
     1741 acagatagag atttggtggt ggaagtcatg gcattagacg ttaatcctga aggattagct
     1801 gttcaagaca tagttacccg atccgttttg gtagccagag aagaggatag cctcattgat
     1861 tcattagaat ttatgaagga aaaaggggtt cgccgattgc ctgttgtcga taatgatcat
     1921 gaactcgttg gtattattac catagatgat ataacggaat tactggcaga gatgttacac
     1981 aaagtagtcg gtgtcgttga caggcagcaa aaaattgaaa aacagcagcg atcttgataa
     2041 ttaatttgga aaaagcgttt gtttttcgga atttattttg tggattgcaa tatttttcca
     2101 ataggcaatg aatattatga acgctatacg tgcttttttt gctatttcac taccgccagc
     2161 gatccaggaa aatatccgga gtaatttgga gccattgagg caaatggcac cagttcactc
     2221 cataaggtgg acaaagccaa tgcatttgca tatcaccttg caatttttac agagactgga
     2281 aacaaaacat attcaggtct tgattgaaaa agtaagtaat gaacttaacc atataaagca
     2341 atttgatttg gaattcggtg ttttagagtg gttcccttct cccaaaaatc caagacttat
     2401 ttcattatct gtagatcctc aagacagact tagggattgg tctcgaatga ttggtcaggt
     2461 ggtggacagt ttaaagtatc ccttagaaaa acgtcctttc agagggcatt tgactgtagg
     2521 tagggtagtg aatttcagcc caaaacttca gcagttgttg caaataaaat ttcctatcat
     2581 tccaccagtt ccgattaata aattttattt aattgaaagt aggcccgata aagaaggaag
     2641 taattatttt cccttggcac aatttgattt aacccggtaa aagagaatgg ctgatttgcc
     2701 aattggaacg attctttgcc tctgtgtcca acttattatg ctcaagtgtc tttattattt
     2761 ggcataggat gaatttaagc caacctatgc caaaaagaga agattatcca taaactaatg
     2821 tttcggagtg agtggaagaa atcgcttttt ccaacgcgtc caattttttc ccgctgttag
     2881 tttggctgaa aaaggtataa ttgccagtag ttgctttgtt aatcaaaata gcggcgagat
     2941 aaccgattcc aagcaagaat acccctaaag taacattggc taacaggtac ttccattctt
     3001 cgcgatgttg atttagcgtt tccttcgagt tttcaatatg ccgtttggca tttattttga
     3061 atgagtttaa ggcacttttt tcatcggact cattattgtc gagataattt tttatctcta
     3121 aacgaagttt ttttgcgagt gtttttgccg ctgttgatgc ttctgtttcc cttctttcct
     3181 ctaattcttt ggccttttgt tcaatttgct gtatttgcaa ataaatagga gacactcttt
     3241 ccattcgcgc aaggtaaatt ccctttttct gttgtaaagc ttgctgatag cgtttttcag
     3301 cctctttact ttgagcaatt atagattcta attgatttgt atttaattgt tgttgtttaa
     3361 tttctttttg tagacgatca atttcctctt gcagatgttt ttgggctaat tgcatttcct
     3421 cattttgttt gagcgaagaa tctttcttaa tgagttcttc tctcaatttt ctaatggttt
     3481 gttctttttc acttgaggat tgatgcaatt gttccaattg aacatttaat tcggtcaatt
     3541 ttttcttaag atcttcaact gtattcttat tatgtaaatt ttccttgctc aaactttgat
     3601 tttgttttcc aagctcttga tttgcagata caagttcctg aatttttatt ttcaattctt
     3661 ccaattgtct tttttgatct tctataatct cttgaagagc aataatttgt ttggattgct
     3721 ggcttatcaa agaggagcct ttttcttttt cctgaattac ctctccaagt tgctgtaatt
     3781 gcaattttaa attgcttact tgctccctaa gtgaagtcac agttcgtttt gctgaagctt
     3841 tctctttctc gctttgctca actctttttt ctttttcttg taattgcttc tcaaattctc
     3901 taatttgtaa ttgtttttct tcaagttgtt cgctaagaga gttggcttgt tttgttaatt
     3961 taagttgatt ttcttcgttt tttcgagtta attcatgcat ctcttttcga agcgattcaa
     4021 gagcagaatc agccaatgat ttttctttag taattctttc aatagtttct tcctggtgtg
     4081 tgttgctttt cttttgtttt tgtaactcct ctttcaagcc ctctacaagt aatgtttgct
     4141 cttgaacttg ttctcttaaa gattttaact gcaattctaa tgtttcttgt ttttgcagtt
     4201 ctagtttaag ctgattaagc tctgaggtta gagattcaat agcagattta tccagggatt
     4261 tttcatgttg actttgtttg tcagcttttt tcagggttag attttccttt tctttctctg
     4321 ctaattgctc ttctaagtgt tgtacttgta attgtttttc ttcgagcgtt ttatgaagag
     4381 agcgttcttt ttttaccaat tcatgcagtt cttttcgaag cgactcaagg gaagaatcgg
     4441 ccaatgattt ttctttgctg attctttgaa tggtttcttc ctgatgcgtg ttggttttct
     4501 tttgcgtttg cagctcctct cctaatcgct tggatagagt cgtttgttca tgtattttct
     4561 ctttgagttc ttttaactgg aattgtaatt tttcttgctc ttgcagttgt aatttaagct
     4621 gccccagttc taaattcaga gagcgaataa tggattgggc ggaagatttc tcatgttcgc
     4681 tttgctcagc cattttctcc agcaactgat atttttcttt tatttggatt aaagtcgctt
     4741 ctagctcctc aaattcaatc gtttgctttt taatttttga ttgaagagaa attactatct
     4801 tttgcagctc atcaatttgt ccagcagttt ttttaccaga aatctgtaat aattgatgcc
     4861 attcacggat tttagctatt ttaccggcgg gatcatctgt gcggccttta aatataccac
     4921 tttcaaaagc agagttaata cgtttccaaa attttttatt actgagaatt ctttcaatta
     4981 aaggcgacgg tttagggcta tttagttctt ccagacaaat cgttgcaagt tgatatccgt
     5041 tcattttagc taatgcttct tcatcaatgg gataaaaaaa ctcactttcc ttattaggcg
     5101 agggctctgc aaaagaaggt gcttttaatg attcttttac atcagcttcc gcaagttgaa
     5161 aagcaatgaa ttgttctttc aatgctttta aattctctgt aactgcctca tctattccga
     5221 atgcttgagc ctgtttgatt atttttagcg tattaataat tccattacct aaagtcagta
     5281 agttttggat tttaacccag gcagagtctt tatgttcctt gcaatagagt tggcatggtc
     5341 cttcaaaagc agcgaaacga gataagttga aagtattagg gtctactgta aatagacggc
     5401 gcaatacaat gctttctttg tagtaaggaa caaaggcatg cccgtgcatc gcttcaccac
     5461 cgctattatc gtagagagcg aacagattag gggtgctgtc tattctttct tttgcccaga
     5521 cattattggc ttcacacgtg atggctagtt catcaacgta aagattaaat tgtttgacta
     5581 attccattaa gttgattgga ttactatcaa acgaaaattt ttccatgtct gtagttttta
     5641 agatgtctgc ccattgattt atggtttctg aaaaagaggt tgaagaggtc agaagaagcg
     5701 tcaagggttt taattccaaa atactttgat acagctcttc aggattgctc aatggggaca
     5761 tatctttaag aacagcaatt tttcctaaac gattttctaa taatgtggcc atggcaatgg
     5821 caaaatcttt gcaattaccc tttgggccaa atgttacctc tttaaatgaa tccatacaca
     5881 tgtaggcggc aagttggatt ttggtttttt cttctatcag atcagctgga atatttttga
     5941 gtgcaccagt tactatttca ataagcaatt caggatttag tttggattgc aattgtcggg
     6001 ctttttcagc catcgcaggg aagaggccat taatcttctt atagttcagg aaataatctt
     6061 tagttagtga tttataatta aaccaacctc tgttttcata agcataaagg agattgtcat
     6121 tattgtttcg atgagcgaaa taacggaagg catctcccca atcgattcgg ccaaattgtt
     6181 tgcttttttc ttctccttct cctttgagta caacgatatt gccactatgt acactatggg
     6241 ctcccagcaa tagagaaaac ataagggcca tagaaaggcc aaaatattta ttttgttttg
     6301 tgatcccggc ataataacct ggcccaaaga aggtttcctt tatcgtatta cgatcactgc
     6361 catcaccgta gcttgttcca atgattttgt gtaattcatc aaatgaaacc agtggctgta
     6421 tcaggccata ggatttgtct tcaaattgaa tgacatcggc gcaaatcaat gatggaaaat
     6481 agctttcatc aatcaatcct cgcttcatga actcttttaa tagcaagcct gcaaataatt
     6541 ccgtgaatag ctcacgaggg tcgcccggtt tttttataaa aaatttccgg ccatcgctgt
     6601 ctttgtaaaa accatcgaca tcacttttat ttttaccacc tgttttttct ttaaatcgaa
     6661 ctatttcttt accttgataa gttaacataa tgactctgac ctaaaacaat actttgtgcc
     6721 attatagcca ccaagtatta actaaacctt aagaaaacct taagaattca tctgcaaatt
     6781 tctttctgct ttggccggta ttgctcacta attcttgctt taacagttgt ttttttacgc
     6841 ttcattagac aataaagacc aacagagctc aaatagggga acccaaagag aaatactatg
     6901 tgctttatcg tcccagccag ggcgatatca gcaactaatc ccattacaaa aataaattga
     6961 aacgaacgat tttttataaa atctgtaata aaaggttgga gcatttttaa tccattaact
     7021 caaatgatag ggacgggaac tagttacata cccatttgtt ctcagcaaaa atggaataca
     7081 cttcattatc cccaatgaca atgtgatcca gtaaccgggc atctaccagg tccagagcat
     7141 ctcgaatgcg ttctgtaatg gctaaatctt gctggcttgc gtcagacaag cccgaggggt
     7201 gattatgtgc cagaattaaa gcagcagcat ttaattgcaa tacacgttct acaatcggtc
     7261 tgggatagac tgttgctgtg ttgatggttc ctgagaataa ttcttcataa gcaatgattc
     7321 ggtgttggtt atccagaaag agtgcggcaa aagtttcatt tttgtagtct cgcagtcgtt
     7381 tttttaaaaa agcataagtt tgttgcgtgt tagataaccg gatttctttt tgcagatcaa
     7441 tgaaatcact acgatgacat atttctttcg cagcctgaag ttgcgcatag cgaacttctc
     7501 ctaatccatg gattgattta aagctttgca gatcagcatt gaggatatta cgtaaatttc
     7561 ccaagtgttt ggtaagctca taggctagtt gcaaacaaga ttttctattg ttacccgaac
     7621 tgatgaaaat ggctaacaat tcgatatcag agaggctatg tactccatta gtaagtaatt
     7681 tttcgcgcaa gtcaagttgc tgcgctgtat gggcaaccat cattttgtat tccttttata
     7741 ttcctttatt gcgaagtttg tgctttgatc ggagttattc ttatcaaaaa tggcgagcca
     7801 tgcaagattt tattaataaa aaaattctac ttggtatttg tggcggcatt gctgcttata
     7861 aatctgctca tctggttaga gaattgactc gtttgggggc tgatgtgagg gttgtcatga
     7921 ctcattcagc acaacagttc attaaccctt tattgatgca ggcattatca ggcaatgaag
     7981 tgaggattga tttattcgat actcaagctg aacgggctat gggacatatt gaattagcaa
     8041 gatgggcaga ttatttgctc attgctcctg cgtccgcaaa ttgtcttgct aaaatggctc
     8101 aaggtcttgc tgatgatttg ctttcaacat tatatctcgt tactgaaacg ccagtaattg
     8161 tttgtcctgc catgaacaga agcatgtggg cacatccagc aaccaaggca aattgtgaac
     8221 tgttaaaaga acgaggtgtg ttttttgtcg ggcctgaaga aggctcccag gcctgtgggg
     8281 aacatggatt gggaagagtc agcgaggcgg atcaaattgt gaataccttg cgcttactgg
     8341 atgtgaatca actattgcat ggcaaacgaa ttgtgattac tgcaggccct actcgcgagt
     8401 cgattgatcc agttcgttat ttaagcaatt acagttcagg gaagatgggt tttgcaatgg
     8461 ctgaagccgc cgctatggca ggcgcacaag tgacattaat tagtggtcct tgtcatttac
     8521 aaacattttc ggacatcgag ttaataagag ttgaatcggc ccaatcaatg ttcgacgaag
     8581 tgaacaggca tttacaacaa ggagatttgt ttattgggac agcagcggta tcagattata
     8641 gcgtgctgaa tcctgcttcg gaaaaaatga aaaaaaatga gaattctgaa ctcactcttc
     8701 agcttgttag aaataccgat attctttctg aagtggctaa aagtggtaaa gcttcctttg
     8761 ttgttgggtt tgcagctgaa acaactgatg tgatttccta tgcaaaagaa aaaatgaaaa
     8821 ataaaaaact ggatatgatt atcgccaatc aggtaggtaa gggaatggga ttcgaaagtg
     8881 atgtaaatca agtaacagtg attacaaaaa acaaacaaat agagctgcca ttaacccata
     8941 aaacacgtct ggcaggccaa attattgcaa tccttgctgc aagtctgcaa aattaggcgc
     9001 aataaaacga ttgagaatta ttatgcatca agttattcag ttaaaaattc tggattcaag
     9061 gataggagat actattccat tgcctgccta tgccactgat gggtccgccg gtctggattt
     9121 aagggtctgt atctcggagc ctatgcaagt agcacctcag caaacagtat tgctacccac
     9181 cggtattgct atttatattg ccgatccaaa gctggcggca gtgattttac caagatcagg
     9241 tttaggccat aaaaatggta ttgttttagg aaaccttgta gggctgattg actcggatta
     9301 tcaaggagag ttaaaaatct cttgttggaa caggagccag gaacatttta ctgttaatcc
     9361 tggtgataga attgctcaat tagtttttat cccggttgta cagacttctt ttgaggtggt
     9421 caatgaattt acagaaagct ccagaggaga aggtggtttt ggcagctccg ggagatatta
     9481 aatgacctat caacaaaagc aaattccccg ctctgtcttt cgtgcctatg acattagagg
     9541 gattatcggt aaagaactgg atgagaattc tttttatagc attggcttag ctattgcacg
     9601 ttatttacaa aaattaaatc gtcgacaaat atttgtagct cgtgatggac gtctgaccag
     9661 ttatgcatta gctacggctt tgaaagaagg tttgctagac agcgggatag atatccttga
     9721 tcttggtgct gtggcgacac ctgtcatgta ttatgctacc catacgcaag ggattgattc
     9781 tggtctgatg gttactggaa gtcataatcc cgctgattat aatggtatca agttagtatt
     9841 ggctggaaaa acattaattc aagatgaaat agatacactt tatgctttgc ttggtgaagt
     9901 caagcagtca gctgttcgtg gcaaagaaac ggcttttgat gtgatagaag actacattca
     9961 gcgtattgtg agcgatattc aattgaagtg ccctttaaaa gtagttgttg attgtggcaa
    10021 tggtgtggcg ggtccaatta ttcccagggt tatttcgaaa ttgggctgtg aggtcattcc
    10081 cctgtattgt gaagtggatg gccgatttcc caatcatcat cctgaccctt caatagaagc
    10141 caatttagct gatttaaaag tggctgtggc aaatcatcaa gccgatattg gtttgggctt
    10201 tgatggggat gctgacagac ttggcctggt gactaataaa ggcgaagtaa tttggcctga
    10261 tcgtttaatg atgttttact caagagagat tttaagtcgt aatccaggcg ctacggttgt
    10321 gtttgatgtg aagtgctcca gtcatttgga gaaagaaatc caggcagcag gcggggttgc
    10381 cagaatgtgc cctactggcc attcaattgt gaaagcggtg atgaaaaaag aacaagctat
    10441 cttggctggc gaaatgagcg ggcatctgtt ctttaaagat cgctggtatg gatttgatga
    10501 tgctttatat agcgcatgcc gattgttaga gattatcagt tcttctcatt tgactgtcag
    10561 tgagcaattt gagttgatcc ctaatagtgt caatactcct gaaataaaaa tagcgattac
    10621 tgatgaagaa aaatttgatt ttattgaacg ttttagtgag caggctgatt tccctgaggg
    10681 caggattttg aatattgatg gtttgcgtgt tgaatttcca aatggatggg gattgttacg
    10741 tgcatcaaac acaactcctt gtctggtcgc acgttttgag gcgacagata aagatcatct
    10801 ggagcaaatt caatatttgt ttaagaaaca aattcagcgc ctggattctg agttggattt
    10861 gccattttag tgtttaggaa gatgaaaaat tcaatatagt gctattctgc ttagagtttg
    10921 tcagatttgg acaggactaa cgaaaaagcc caaggttcat tttttaataa agaggttagg
    10981 ggttttcgta agttctgttg gaaacaaaac gagatgttga cttcttaaca caatacaaca
    11041 tgacttccag cacttgtttg ttttgtgttc ggcctggtat tttctcttgt ttgataagat
    11101 gaaattggta tttattgatt aagctctcta tgtattcggg ttgatggcta aatcgagcgc
    11161 tgctttccag tttccacgga gttgttgtgc tgatttcggt agtgaaaata aaataccctt
    11221 cttgcttcag atgttgattg atgtaattaa aaatatcatc cagattacca aaatagggta
    11281 ggacgtctgc agcgactgct aaatcgtaat cgtttttatt ttttcttaaa aaatcgatta
    11341 attccgagca taccagaaga tcatagatgt ttttttcctt tgctctagct atcatttttt
    11401 ctgctatatc tactccggtt aagtgtttgc ttatttcacg aagcacaata cctgttaatc
    11461 cagtaccaca acccaagtcc aaagaatgac tggtttgcaa aagttgaagt tggtggatga
    11521 ggcggccaat gtgatgcgga atcttatagt gtaattcacc ttgcatgtgt tggtcgtaat
    11581 acaaggcata attattaaat aagttttggg catatttttc tgtagtatta acgctcgttg
    11641 ctcctgtaat ggcattcagc atatgcctgc tgaccacatc atcggggttg attaccagag
    11701 cgcgctcaag atattcgcgc gcggtttctc gcatgtccat ttttaaataa atagcggcca
    11761 gattattcaa ggatggggtg tgatcacttt gtaatgttaa aatctgatcg aacaaaaggg
    11821 tggcttcatt gagatggcct aatgccattt gagcgactcc ggaattgtat aaatactcca
    11881 gattattggg ttcttttttc agtaaaacat cgtaatgcat gagcgcattt tcaaatcgat
    11941 catgatgcat gaatgtggct gccaaattat tacgtgcatc aatgtcttca ttatccaaag
    12001 ccaaggcttt agtaaaataa tcaacagcta attggttttg ttctcttttc aaagcgatca
    12061 cgcccaagtt aattagggat tgaacatgtt catgatcttg ctctaacact ttatgaaatg
    12121 cctgttctgc ttctgagagt aaattgtctt ccaaatgcaa aatacctaag taaaattgag
    12181 cttctctgtg cttaggattt agagctatca cattattaaa ttgtgttttg gcagcagaca
    12241 attgttgatt tttgagaagt aacaaaccta aattaaagtg agccgcgcta aagtcaggtt
    12301 ctgtattcac tgcaatcaca taatgatgca acgctttctg gtaattgttg agtaatgcgt
    12361 aggttgctgc cagattatta tgagcctgga catactctgg tttaatctca atggcttgct
    12421 ggtaatattt tattgcctca tctaattgac ctgccttttt ataagcgtta gcgagattat
    12481 tgagtatacc aacgtcattg gggttaattt ttttggcttg taaaaaatat aaaatggcat
    12541 tttccatatc acccaattgg gcataggtta atcccaggaa atgcagagtt tccgtatgtt
    12601 tgggtgattg ggcaagtatt tgctcatata agctaatggc ttgaggtaaa tggccttcat
    12661 attgaagctt ataggcttgt gcaaaaagag tatctacttc actgttcata ttaatctgga
    12721 agaagggcgc gtaattggtt taaagctttt gcgcgatggc taatcctatt tttaatttta
    12781 gcaggtaatt cagcggctgt gcattggtat tcgttgagat aaaataccgg gtcataccca
    12841 aagccatttg ttcctgaagg tttcacgcta ataatgccat gaaatatgcc tgtggcaatg
    12901 atcggtgttg gatctttggc atgttgcatt agcgctattg cgcaaaaaaa ataggcttgt
    12961 ctttgttctt gggataaatc agccatttta cttaacaatt gttgaatatt gtcttcgtca
    13021 tttgcctttc ttccagcata gcgtgctgaa tagatacccg gctctccatt taaagagggg
    13081 actacaagcc ccgagtcatc ggctaaagct ggtttattgg ctaatgagct ggcatgacgg
    13141 gctttgagaa tggcattttc tataaaactt aatcctgttt cttccgcatc gaatataccc
    13201 aagtcggctt ggggaatgca tatggtgggg gctagtaatt gttctaattc ttttattttt
    13261 ccagggttac tggttgccaa aataatttct ttcattctta ttccatgtta ttaattcagt
    13321 attcataaaa aggataatta tgcatgagaa ttttggaaaa aaatatcaag aagactccct
    13381 ggtatttaaa accattcttc tggaatcaaa aaagaaaata cggccaaata ctagaacccg
    13441 ctttgatttg ggcaagaatt cccaggttat ttttggcagt tgctgctatg tatggcgtga
    13501 tagacagaaa aaaatcacca ctttccccaa tattacgctc tctcatcact gtcagagttt
    13561 ctcaaattaa ctggtgtgcg ttttgtgttg atattaattc agcaacttta attaaaaggg
    13621 ctggaagtga agcacagtat aaacaactgg cgaaatggag gagttcctca ttattttcac
    13681 cagaagaaaa agcggcattg gattatgctg aaaaagtaac ttattctgat caacgtcctg
    13741 atgatgagtg cttcaatcaa ttgaaattgt attattctga acaggaaatc attgaattga
    13801 cagcactgat tgcatttcag aatatgtcca gtaaatttaa tagtgccctt ggcgttgaag
    13861 cacaaggatt ttgttcgagg caactgactg gcaaaagctg aatcgtcctg ccagtcagta
    13921 ctttcaatta catagagatg cttttaccta ttgtattgtc gttgactcga tcatgcctta
    13981 gttgttgttc atctgtaata agatcaacta ccgtgttaaa atcctgggcc tcatcttcag
    14041 taatttctat tgaatcagct gcttgtaagt aatgtaattg actcaaatcc tgttgaatgt
    14101 ctttaatgcc tttttgggga tgtttttcta tgtaagcatt cgatctggca atatagtcat
    14161 ttacaaaacc ttgatacacc gattgtccat ccagcatttc cagctcgcac aaatcagcaa
    14221 agtgagcatt gacccttcca aatttatcga ctccaaattt ggaagccaga cgttctttag
    14281 cgctttcctt gactcgaaca taatgtttcc tgtccagctc atcacgatct tttcgatact
    14341 cttctggaca ggtatccgta ataggagctc ttttttgaga tttggtttgc ccagtttgta
    14401 tttcatgttg atacttgctg actttacttg ataatgtgga agattctaat gtttgatctc
    14461 ccaagtgttg aatatgacat tggtaaggat cagcatgttt atattttttt cctggagtaa
    14521 tatgccagcc agtaaattct aacaatcttt taacaatgct ttttaatatg ttaggtagcc
    14581 atggtgattg ggtaatcata tcgcaaacgg ctgctttaaa tgacttgaaa acattgttca
    14641 tgattgcacg aactttgatg tctgcctggt cttctaattg cttttttacc tctaatgcaa
    14701 tgctagcccc gtagcaatcg ccctgtaaaa taatatcatc ggggtgcacg ccttgtttaa
    14761 tcaaagaaga taccacagaa ataccggcat tgattaagtc atttgcctct ctcacctttc
    14821 ctgaactaag tcctgttcct ggaaaattaa aagcatgtac tgttgctcca gtttctttgc
    14881 aagctgtgct gatatcacga aaacaggctt ggtaataagt atttgctccg ggaaaataga
    14941 caatgtgttt tccggtgccg ggctttttag gttcatggga ctctggttgg catttcatga
    15001 cttctaactg acaggattca ccagaaacca gctttgtttt cactttcttc agacttaaat
    15061 ccaggccttg aaattcttta taatagtggg attttttatc ggttttttgt ttttcaacga
    15121 aatctctaaa aggagcttcg tcatcaactt cgcccttttt tccgtagcct ttgtttttat
    15181 accaatcacc agcccctgct ggaaaaaggg aattagctac aaattgttgt aaacgactat
    15241 gaaaaaaact gtcttttgta atagttttgg ccatttaatt ccccagtgtg agcaaaataa
    15301 agtcagtata ttttttaaat ttagcataaa tttagcagaa aaaacttaag agagtattaa
    15361 taataaagta cattcggatt tgctaaaaac caaaaa
//
LOCUS       DAXLDT010000069        15263 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXLDT010000069 DAXLDT010000000
VERSION     DAXLDT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.069"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..299)
                     /locus_tag="ACF2SP_001731"
     CDS             complement(<1..299)
                     /locus_tag="ACF2SP_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359517.1"
                     /translation="MTPWFITLPWLHYLDFTYNEVYHEARFEKDKRYYVLRLEKDLLD
                     DWTITAINGRIKSKLGQRRILDFSTYTNAFAQFLIMIKIRYQHNYRLKILYSENI"
     gene            complement(300..884)
                     /locus_tag="ACF2SP_001732"
     CDS             complement(300..884)
                     /locus_tag="ACF2SP_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2359518.1"
                     /translation="MPKEGKARVLSEAEFKRLLIVAKDGQFAIRNSALVYCSFGLGLR
                     VKEIASLTIADVADYNYQLTEEICLKRSMTKGEKQRYAYLANEKIRKALQAHLDTLKP
                     VARDKPLFQTQRKSRFTPNSLQKWFKSLYDKAGIIGASSHSGRRTFITRLIEQGADIK
                     AVSRLAGHANIVTTAIYVEDNPDRLKRIANLAIF"
     gene            984..1442
                     /locus_tag="ACF2SP_001733"
     CDS             984..1442
                     /locus_tag="ACF2SP_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HFD2359519.1"
                     /translation="MNTLIDAAIKYLSNHYCSEKELIRQLERDFSHVPELDAQIHATI
                     ARLRELHLINDNRLAESLSARYIHKGNRFIQQILKQKGVKEEVIEQILEHIELEEVRA
                     LDEARKKMRGFKNDTDETINTKLARFLSGRGFSHATIKTVLKQLSEEQTT"
     gene            1525..2067
                     /locus_tag="ACF2SP_001734"
     CDS             1525..2067
                     /locus_tag="ACF2SP_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HFD2359520.1"
                     /translation="MINLQRIMIIGRPGSGKSTFSVKLQKSLNIPLFHLDKYFFIADW
                     VPREYEDFLSLQKELVANPCWIIDGNSSKSFEIRYREADMCLYFNLPKWLCYWRVFKR
                     LFYKASEIDDRAAGCNETVRWSLLSYMWNYEQRINPLLNALKNQYPKVKFIELRSPYD
                     VAHLMRVLTHKEEYSIVTGF"
     gene            2071..3207
                     /locus_tag="ACF2SP_001735"
     CDS             2071..3207
                     /locus_tag="ACF2SP_001735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359521.1"
                     /translation="MMPTNMMILLMGIAGTGKKTIGEAITTLASQFRLAHHHAWIDPV
                     LKLLGNDDQVFWSLDDKGWAALNKARDVIFDTMTEVCSKETSFVITYELLANNSWHQE
                     FFNQVQAVAQKREAMFVPIRLICNGAELVKRLKYSDRKGYFKTQDEALITKRLFEEDV
                     YFSKEPYEMTLDVSCLSAEESARRILDWTSLVYGSKNQNLEFKPLGQRDLNLMTQWFA
                     EPTVKQGYARNQQFSLEDISAKYSPRIEGIECIPSFIIYLNQKPIGFIQYYCLSDHLP
                     EGISGHNASLFDEATPEQLAGIDLFIAEPSFRGVGLGRQIIRRFIAEQLSKFKAVVVD
                     PQIGNEQAIACYQKAGFLPTQYSEDANYLLMINMLSYRGSYEYS"
     gene            3194..3760
                     /locus_tag="ACF2SP_001736"
     CDS             3194..3760
                     /locus_tag="ACF2SP_001736"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359522.1"
                     /translation="MNILETSRLILRTWREADLDPMSLIDQDKKVCQFLPGIGNRSAT
                     KAGIERMIAHDKEKGFSLYAVELKNTGEMIGFLGLMTPSFEAHFTPAVEIGWRLSSTH
                     WNQGYATEGAKAVLHYAFTSLNLPEVVSFTAVNNLASRRVMEKIGLQHNTKDDFDHPK
                     LEHNSPLKRHVLYRLSRADYLNSKSIQK"
     gene            3757..4710
                     /locus_tag="ACF2SP_001737"
     CDS             3757..4710
                     /locus_tag="ACF2SP_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2359523.1"
                     /translation="MIWETTLKKITDETANRAANKWVKNPTHLKLINNQINCVYRFES
                     KNQGFYLRMTHEKIRKAHELLSAIDFQKHLYLHQVPICEPVVSQEGNDVETVHQDDLE
                     FFVHVCREVPGQIMNFDYPDKKAYLTWGRALGLLHQASQSYVASEHHFLTWEDLWRET
                     WDYARQEEALIQDLYQTITTRFKTFSINSAHFGLTHGDHRPGNVLYDGESVHLIDFDE
                     PVYHWYLADIAKPFLDLCNKPWPLWKPLFEWFIEGYRQIRPLSSDELKEMNHFSQMKS
                     LDIYLWCKYNWFEETAPGGKPRNEWLHDLKTMALTPLFYVP"
     gene            complement(4715..5290)
                     /locus_tag="ACF2SP_001738"
     CDS             complement(4715..5290)
                     /locus_tag="ACF2SP_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFD2359524.1"
                     /translation="MQEISKRIAKTLKSLRQERGWSLDKTALETGVSKAMLGQIEREE
                     SSPTISTLWKIASGFQASFSSFIEDSLDNSTNPVYRAGHAETLHPDDEKIRVLPLFPF
                     DEQLHFELFVIELLPECEHLSPPHKHGVIEHVIVVDGTIELLLGGSWKKLSKGEGLRF
                     NANQPHGYRNPTNELSRIHDIIHYPPQYKSP"
     gene            5394..5816
                     /locus_tag="ACF2SP_001739"
     CDS             5394..5816
                     /locus_tag="ACF2SP_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HFD2359525.1"
                     /translation="MVEHPFKNKLVAVLNKRIEPGKVMNALAHMCIGLGSVIGEEELR
                     LTDYRDADGGSHPYISEIPFIILCENPNKIRTLRQNALAKNVLFNDFTDTMTVGTYQE
                     QIERTAQVKENDLIYYGIVLFGDWDVVTELTRKCSLWR"
     gene            5833..6324
                     /locus_tag="ACF2SP_001740"
     CDS             5833..6324
                     /locus_tag="ACF2SP_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFD2359526.1"
                     /translation="MNEGKNLSKSAQIIQDFLSQKGISCEVKELDSSIRTAKDAADTL
                     GCGVAQIVKSLLFCTEKTNKPVLVLASGVNRVNESLIGNLINESIGKADADFTREITG
                     FAIGGIPPVGHKNVINTVLIDEDLLCHEVLWAAAGTPNAVFSLSPEALKQLTNGIVVK
                     IRE"
     gene            6328..6963
                     /locus_tag="ACF2SP_001741"
     CDS             6328..6963
                     /locus_tag="ACF2SP_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HFD2359527.1"
                     /translation="MQKIFWDNPYQRQLMTKVESANENRLLFAETIGFSFSGGQESDT
                     VRVNGLMVTHSEIEDDLIYYTLPSEHGLSEGDVVLMEIDFVRRYKLMRLHFAAELIFE
                     LVQRMFPIEKIGAHIAEHKARIDFNYQHNISDIFDTLLLEYNQIIAKDMSIRTGFSDE
                     KSQRRYWEIEGFSKVSCGGTHVKSTAEVGYITLKWANVGKGKERIEIYLVQ"
     gene            7031..7474
                     /locus_tag="ACF2SP_001742"
     CDS             7031..7474
                     /locus_tag="ACF2SP_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359528.1"
                     /translation="MNAQEITVLNDLKNDINQLLGFHEETPRINYGPCGAFAKLFFDA
                     WNSRFKDKVHIVFVMVTSHEECWHIALRMPSGELYDGGIGLHCEEVYGEGYLFEDMIE
                     YDHERLEKWSYGLERDYPRFCPDFNKEVVNSLITHHLDRLQSQGI"
     gene            7580..7960
                     /locus_tag="ACF2SP_001743"
     CDS             7580..7960
                     /locus_tag="ACF2SP_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HFD2359529.1"
                     /translation="MQAQIFDENIAMKQEHQLIYELEISLLNPQTRKSITQLKLLIAD
                     EFIEYGASGLIYNKNDLLDSLPEEEPQSYMVNDFSVLEVSSEVMLATYRVTVASKSSL
                     RSSLWQYKHNRWQMVFHQGTPCQE"
     gene            7985..8452
                     /locus_tag="ACF2SP_001744"
     CDS             7985..8452
                     /locus_tag="ACF2SP_001744"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359530.1"
                     /translation="MITIAYLKHHSDCIPELAKIWHEVLGQIWVPDVPTSRVEENLRN
                     HLNIEQLPLTIVAFDEDKPVGMCSLRVNDGIRPELTPWLGSLVVSPSHQKQGIARQLI
                     HRVKEEAQALGFKNLYLFAFDPTLPEYYSRLGWCMIGMDEFKGHPVTVMEIML"
     gene            8449..8901
                     /locus_tag="ACF2SP_001745"
                     /pseudo
     CDS             8449..8901
                     /locus_tag="ACF2SP_001745"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
     gene            8944..9699
                     /locus_tag="ACF2SP_001746"
     CDS             8944..9699
                     /locus_tag="ACF2SP_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2359531.1"
                     /translation="MKKLDTYQSLCTEVYDLSKPNAPQDEYSFYRSYAAEVKGLILEP
                     MCGTGRFLLPLAAEGFDVHGFDASQPMLERLHAKAKSKNLKPKAWHGFIEDLNQSAKY
                     SLIFIPSGSFGLITEKVDIQKALKTIYEHMEDKGLFVFEVETRYAVPKELGIWRGTRW
                     PKEDGTIILLSQLAMLDEEICYSIGKYELIDNNRVIQTEVEEYKIRIYQDLSFLLNLL
                     NEVGFSNVRTVKAFDRNASPNETDESIVFECRK"
     gene            9712..10605
                     /locus_tag="ACF2SP_001747"
     CDS             9712..10605
                     /locus_tag="ACF2SP_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFD2359532.1"
                     /translation="MNILCPEPLQRGDIVGLISPSSPITEKAIEVGVHLLKSNGFNVK
                     YAKHMFASERFLAGKDRDRAQDVMDFFIDSEVKAIIATRGGQGSQRLLPFLDYELIKK
                     NPKPLFGFSDTTALQLGLFKNTGLVSYTGFTLTIHLSTQVKKTLFSSLLGQEYTIRKG
                     IKVHGGVSRGTLLGGNLTLMTNLMGTPYMPTFKGCILLVEDVGVEPYNIDGMFSQLDL
                     AGIFDEVSGVIFGTFEQCKSKKSNQSDGTVEDVINEWASKLKVPCIKEFSYGHGKQNC
                     ILPIGKVVTLDADTTCVTIQN"
     gene            10665..11060
                     /locus_tag="ACF2SP_001748"
     CDS             10665..11060
                     /locus_tag="ACF2SP_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HFD2359533.1"
                     /translation="MNTQEIPKEYQHTSPAGAEVRLLMNNHLGGMAHCTLKAGTISKA
                     VRHKTVSEFWHVLSGEGAIWRKLNDEESITPLTAGVSIDIPLGTHFQYRSDAADLVFI
                     CVTMPPWSGSDEASYVEHGAWPPTVEYIK"
     gene            11057..11914
                     /locus_tag="ACF2SP_001749"
     CDS             11057..11914
                     /locus_tag="ACF2SP_001749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359534.1"
                     /translation="MTLKLSFLENPNPDDVQILTNGIKAYAKQARGFESLDFFACFIR
                     DADNSIVGGCSGGTLYGGLHVDNLWVSESIRHQGWGTKLMQEALKYGNEKGCAFATVN
                     TMDWEAIEFYKKLGFELEFERHGFQKNSIFYFLRKEFQGTRDIGLLNDDSVSDLSQNL
                     KSSISESVVREEVVSISNAPHFKWGQNCDAWWLKNDGQFNVLYETMPSGSFEVKHYHQ
                     ETEQFFYCLQGQLVIEFEDYEQVLQEQEGISIKAGITHKVKNSSGNLVSFLVISSPNL
                     IGDRVNLES"
     gene            11916..12368
                     /locus_tag="ACF2SP_001750"
     CDS             11916..12368
                     /locus_tag="ACF2SP_001750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359535.1"
                     /translation="MSSELIIRKALIDDVQHLHPLMEQLGYPQSSEELQRRLELFTSE
                     LHYGVLLAEIAGKVVGWIAWSKSYLFVSETTRFHIEGLVVDQSYRNQGIGKKLMITVE
                     EFARQFSPCIIDLTSGLRRAKEGSHDFYKSLGYQNEGHMAKLYLRKML"
     gene            12365..13402
                     /locus_tag="ACF2SP_001751"
     CDS             12365..13402
                     /locus_tag="ACF2SP_001751"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359536.1"
                     /translation="MNIKELQSALKVETATLDDYPTIQNMARFYVYELSRDCGFISDD
                     WALPSDGLYESFDFKKYFEEPTRKAFLIKVGNKLAGFALLNHEGVYPDTAWNMGEFFI
                     TAKFQGKGLGYLVSSELWKMHPGLWEVSVIPENKRGLSFWRKAISSVTAGNYQEEIKT
                     INYDTHQPQRVIFRFDSTAHECSEPLESENEYQIQFVDELDEATEKRMTKGFIAYEAQ
                     HGIDVNYRRFSVTISSAQGKIFGALNAFTAFAEIYVDDIWVDSAYRGKGYGKQLLLAL
                     EHHFKGQGFNNINLVTSAFQAPEFYKKCGFIAEFTRINKKNPKLSKTFFVKFFNEESE
                     TQGTLKAAHGE"
     gene            13395..14408
                     /locus_tag="ACF2SP_001752"
     CDS             13395..14408
                     /locus_tag="ACF2SP_001752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFD2359537.1"
                     /translation="MNKNQLETICKKLKMNQPCDLPQRVHGGLLHKMWYLKCNGSQFA
                     VKQLNKRLQLTEEVKSQYELTEEIAFQFSQCTIPAVHALKLENNYLILSEDEAFLVYP
                     WITAKTLDKDAISEMHALKIATLLAKIHRINLNVPQLSAQEFDIHPNEKISVLIDQSA
                     SKNLVFKEQLKSNQRVIIELNDIYQKAIPILQSSSVVSHGDLDQKNVLWDKNDTPFLI
                     DWESARKLNPTYEIVNCALDWSGITTPLFNKTLFIKMLKTYASSGITINQDHLEAAFY
                     GVLGNWINWLVYNINRSFSSDKEQQDIGIEQVLQVLPTIIQVKASIPELTQLVTHELG
                     ITF"
     gene            14469..>15263
                     /locus_tag="ACF2SP_001753"
     CDS             14469..>15263
                     /locus_tag="ACF2SP_001753"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2359538.1"
                     /translation="MTNINNNKAALVTGASHGIGLELAKILLGDGWVVYGTGRDVSSL
                     EDTKALFPRFVPIQSDFTRNSDIEQVAKTINDSGIPLHLLVQNAGMKSPPRPLTEYDC
                     DSIDEVFQVNLLAPMKLTALLAAQMPEKSRILFVTSRAATLKLKESSTYCASKAGLDE
                     VTAIVRKELAEKNIGVSCIIPGEVDTRIQKILRETTSFHLHKMFDEAYQSGQLISPET
                     CAEFLKWFLCDLSFDEFKQSNMPVSIYEEWHHPFWLKDRNQLPPFPF"
BASE COUNT         4797 a         2573 c         3211 g         4682 t
ORIGIN      
        1 aaaatatttt cactatacaa aattttaagt cgataattat gctgataacg gatttttatc
       61 atgattaaga attgagcaaa cgcatttgtg tacgttgaaa aatccaatat tcttctttgt
      121 cccaacttgg attttattcg tccgttaata gcggtgattg tccaatcatc tagtaaatct
      181 ttctctaaac gaagtacata atagcgtttg tctttttcaa agcgtgcctc atggtagact
      241 tcattgtagg taaaatccaa atagtgtaac caaggtaaag taataaacca tggtgtcatt
      301 tagaaaatag ccaagttagc aatacgtttc aggcgatcag ggttgtcttc aacataaatt
      361 gcggtggtga caatgttggc atgacctgca agacgagaga ctgctttaat atcagcgcct
      421 tgctcaatta agcgggtaat gaaagtacgt cgaccagaat gggagcttgc cccaatgatt
      481 cctgccttat catagagtga cttgaaccat ttttgcaaac tattgggtgt aaagcgactt
      541 ttacgttggg tctgaaataa gggtttgtct cgtgcaacag gttttagggt atcgagatga
      601 gcttggagtg ctttacggat tttttcattg gcaagataag cgtaacgctg cttttcacct
      661 ttggtcattg agcgcttcaa acaaatctct tcagtcaatt gatagttgta atcggctaca
      721 tctgcaatag tgagagaggc gatttctttc actcgcaatc caagtccaaa agaacaatac
      781 acaagggcac tatttcttat agcgaattgc ccatctttgg caacaataag cagtcgctta
      841 aactctgctt cacttaagac tctggccttt ccttcttttg gcataaaaga ctcaatattt
      901 tcctgttata atggtattct aaataaatca ggctgtcttg gcaaaagcaa aaataaaatt
      961 gtggtttata gtagagcttt agtatgaata cactcatcga tgcagcaata aaatatcttt
     1021 ccaatcatta ttgcagtgaa aaagagctca taaggcaatt agagagggat ttttcgcatg
     1081 tacccgagtt agatgctcag attcatgcaa ccatagctcg ccttcgggaa ttacatttaa
     1141 ttaatgataa tcgtttggct gaatcattaa gtgcccgtta tatccataaa ggaaaccgtt
     1201 ttattcaaca aatattaaaa caaaagggcg ttaaagaaga ggtgattgag caaatcttag
     1261 aacatattga actggaagaa gttcgcgccc tagatgaagc tcggaaaaaa atgcgtggat
     1321 ttaaaaatga tacagatgag acgataaata caaaattggc tcgtttctta agtggccgag
     1381 gtttttccca tgctacgata aaaaccgtac ttaaacagtt aagtgaagaa cagactactt
     1441 aaagttttag atagtgtagt ggatgagtac aacaatcatt tgcatcctta agaaaagaac
     1501 acagtaaatt aaactctgaa tgaaatgata aacttacaac gcatcatgat tattggtcga
     1561 ccaggaagtg gcaaatccac tttttctgtt aagttgcaaa agagtctaaa tattcccttg
     1621 ttccatttag acaaatattt ttttattgcc gattgggtgc cacgagaata tgaggatttt
     1681 ttgtcactcc aaaaagaatt agttgctaat ccttgctgga ttattgatgg taattccagt
     1741 aaatcattcg agatacgtta tcgcgaagcc gacatgtgct tgtactttaa tttacccaaa
     1801 tggctatgtt attggcgggt atttaaacgc ttgttttata aggcttcaga aatcgatgat
     1861 agagccgctg gttgcaatga aacggtacga tggtcgctat taagttatat gtggaactat
     1921 gaacaacgga ttaacccttt attaaatgct ttaaagaacc aatatccaaa agtgaaattc
     1981 attgagctgc gtagtccata tgatgtggcg cacttgatgc gtgtgcttac acataaagaa
     2041 gagtacagta ttgtgacagg attttaataa atgatgccga cgaatatgat gatactcttg
     2101 atgggtattg caggtaccgg taaaaaaacc ataggcgagg caattacaac actggcttct
     2161 cagtttcgat tagcgcatca ccatgcttgg atcgatccgg ttctaaaact tctaggtaac
     2221 gatgatcaag tattttggtc tcttgatgat aaaggatggg cagcgcttaa caaggcccga
     2281 gatgtgattt ttgatacgat gactgaggtt tgttctaaag aaacgagctt tgttattacc
     2341 tatgagctct tagcgaataa ttcttggcat caggagtttt ttaatcaagt acaagccgtt
     2401 gctcaaaaac gggaagctat gtttgttcct atccgattga tttgtaatgg tgcagagctt
     2461 gttaaacgct taaaatattc agatcgaaaa ggctatttta aaacacagga tgaggcactc
     2521 attacaaaaa gactgtttga agaagacgtt tattttagca aagagccgta tgaaatgact
     2581 ctagatgtta gctgtctttc agcagaggag tcagcgcgtc gtattttgga ttggacttcc
     2641 ttagtttatg ggagtaaaaa tcagaatctt gaatttaaac cattgggtca aagagatttg
     2701 aacttaatga cccaatggtt tgcagagcct acggtaaaac aaggatacgc acgaaatcaa
     2761 cagttttctt tggaagatat tagtgcaaag tactcacccc gaattgaagg cattgagtgt
     2821 atccccagtt ttattattta tttaaatcaa aaaccaattg gttttattca atattactgt
     2881 ctttccgacc atttacccga aggaatttca ggacataatg cttccttgtt tgacgaggca
     2941 acacctgaac aattggctgg tatcgacctt tttattgcag agccatcgtt tcgtggagta
     3001 gggttgggca gacaaattat aaggcgtttt attgccgaac aactctccaa atttaaagcc
     3061 gttgtggttg atccacaaat tggaaacgag caagccattg cgtgctatca aaaggctggt
     3121 tttcttccca cgcaatacag tgaagacgct aattacctac tcatgataaa catgctttct
     3181 tatagaggct cctatgaata ttcttgaaac ttcccgtctg attttacgta cttggagaga
     3241 agctgatctt gatcctatgt cacttattga tcaagataaa aaggtatgcc aatttttacc
     3301 cggaataggg aacaggagtg ctacaaaagc aggcattgag cggatgattg cacatgataa
     3361 agagaaggga ttttctttgt atgctgttga actaaaaaat actggagaaa tgataggatt
     3421 tttagggtta atgacccctt cttttgaagc gcactttacc cctgctgttg aaattggctg
     3481 gagactttcc tcaacgcatt ggaatcaggg ttatgctacg gagggagcaa aagcggtttt
     3541 gcattatgca tttacctctt taaatcttcc cgaagtagtt tcatttacag cagtgaataa
     3601 tctagcttca agacgtgtta tggaaaaaat tggtttacaa cataatacaa aggatgattt
     3661 tgatcatccc aaattggagc acaatagtcc attgaaacga catgtgcttt atcgattatc
     3721 cagagctgat tatctgaaca gtaagagtat ccaaaaatga tttgggagac cacattaaaa
     3781 aaaattacgg atgaaacggc aaatcgtgct gctaataagt gggtaaaaaa ccctacccat
     3841 cttaagctta tcaataatca aattaactgt gtttatcgat ttgaatctaa gaaccaaggc
     3901 ttttaccttc gcatgacgca tgaaaaaata cgaaaagctc atgaactttt gagtgccatt
     3961 gattttcaga agcatttata tttgcatcag gttcctattt gtgaaccggt tgtatcccaa
     4021 gaaggaaatg atgtcgaaac ggtacaccaa gatgatttgg aattttttgt tcatgtttgc
     4081 cgtgaggtgc cagggcaaat catgaatttt gattatccag ataaaaaagc ttatctaaca
     4141 tggggccgtg ccctaggtct attgcatcaa gcctcgcaaa gttatgttgc atcggaacat
     4201 cattttttaa cttgggaaga tttgtggcgt gaaacttggg attatgcgcg tcaagaagag
     4261 gctctgatcc aggatttata ccaaaccatc acgacacgct tcaaaacttt ttcaatcaac
     4321 tcagcacatt ttggcctaac ccatggggac catcgtcctg gcaatgtgct ttatgatggt
     4381 gagagcgttc atctgattga ttttgatgag ccagtttatc attggtatct tgccgatatc
     4441 gctaaacctt ttttggactt atgcaataag ccatggcctc tctggaaacc cctgtttgaa
     4501 tggtttatag aagggtatag gcaaatccgg ccattgagtt cagacgaatt aaaagaaatg
     4561 aatcattttt cgcaaatgaa aagcttggat atctaccttt ggtgtaaata caactggttt
     4621 gaagaaacag cgccaggagg aaagccacgc aatgagtggc ttcatgactt aaaaactatg
     4681 gcattaacgc ctttgtttta tgtaccataa tcgattaagg cgatttatat tggggtgggt
     4741 aatgaataat atcatggatg cgcgatagtt cattggttgg attccggtag ccatgcggtt
     4801 gattggcatt aaaacgaagc ccttctcctt tagaaagttt tttccagctc ccgcctaaca
     4861 aaagctcaat ggttccatcc acaacaatga catgctcaat gactccatgt ttatggggag
     4921 gtgaaagatg ttcacattcg ggcaataact caataacaaa caattcaaaa tgtaactgct
     4981 catcaaaagg aaatagaggc agtactcgta ttttttcatc atctggatgc aaggtttcag
     5041 catgtcccgc cctataaaca ggattggttg aattatccag actgtcttca ataaacgaag
     5101 aaaaggaggc ttgaaagccg ctcgcaattt tccataaggt agaaatcgtt ggacttgatt
     5161 cttctcgttc aatttgcccg agcatcgctt tagaaacgcc ggtttcaagc gctgttttgt
     5221 ctaaactcca acctcgttct tgtcttaatg attttaatgt ttttgcaata cgttttgaaa
     5281 tttcttgcat gatatctctt aataaaaaag cttgtgcgtt ttagcgcacg ggcatatact
     5341 ggtttattat gcgttataac gcacgaattg ggaagtagat tgtggagaac tgtatggttg
     5401 aacacccttt taaaaacaaa ctggttgcgg tacttaataa gcgtattgag ccaggcaaag
     5461 tgatgaatgc gcttgctcat atgtgtattg gtttaggttc cgtaattggt gaagaagagc
     5521 tgcgtttaac cgattatcga gatgcagatg gaggatctca cccttatatt tcagaaatac
     5581 cttttatcat tctttgtgag aaccccaaca aaattagaac attacgccag aatgctttag
     5641 caaaaaatgt tctttttaat gattttaccg atacgatgac tgtgggtacc tatcaagagc
     5701 aaattgaaag aacggcacag gttaaagaga atgatctgat ttactatggc attgttttgt
     5761 ttggagattg ggatgtggtg acagagctga ctagaaagtg ttctttgtgg cgatgatggg
     5821 tgggtgatag cgatgaatga aggtaaaaat ttaagtaaaa gcgcgcaaat catacaggat
     5881 tttttatctc aaaaggggat atcgtgtgag gtcaaggaac ttgattcgag catacgcact
     5941 gcaaaagatg ctgctgatac tttgggttgt ggtgttgctc aaattgtgaa atcactatta
     6001 ttttgtactg aaaaaaccaa caagccggta ttggtattag caagtggtgt taatcgtgtg
     6061 aatgagagcc tgattgggaa tctcatcaat gaatctattg ggaaggctga tgcagatttt
     6121 acacgagaaa taactggttt tgcgattggt gggatccctc cagttggcca caaaaatgtt
     6181 attaatacgg ttcttatcga tgaagatttg ttatgccatg aagtcctctg ggccgcagca
     6241 ggaacgccca acgcagtatt ttcattatct ccagaagcac ttaagcagct aaccaatggg
     6301 atagtggtaa agattagaga gtaagttatg caaaaaatat tttgggataa tccctatcaa
     6361 cgccaattaa tgactaaggt ggagtcggca aatgagaatc gactactttt tgcagagact
     6421 attggttttt cgttttccgg tggtcaagaa agtgatacag ttcgcgtgaa tggcctgatg
     6481 gtcactcatt ctgaaataga agatgacttg atttattata cattaccttc ggagcatggg
     6541 ctttctgagg gcgatgttgt ccttatggaa attgacttcg ttcgtcgcta caaattgatg
     6601 cgtcttcatt ttgctgctga attaatcttt gaacttgtcc aaagaatgtt tcctattgaa
     6661 aaaataggtg ctcatattgc tgagcataag gctagaatcg attttaacta tcagcacaat
     6721 atctcggata tctttgacac tctcttgctt gaatacaatc aaattattgc aaaagatatg
     6781 tctatacgca ctgggttttc tgatgagaag agtcaaagac gttattggga aatcgaagga
     6841 ttctctaagg tttcttgtgg tggaacacat gtaaaatcaa ccgcggaagt gggctatatt
     6901 acgttaaaat gggcgaatgt gggtaaaggt aaagagcgaa ttgaaattta tctggtacaa
     6961 taaatcagcc taaacgtaga gtgttggatg ttagccaaaa aagaagtttt taataccata
     7021 ggaattacgt gtgaatgcac aagaaattac agtcttaaat gatttaaaaa acgacataaa
     7081 ccaattgctg ggatttcatg aagaaacacc aaggattaat tatggtcctt gtggtgcgtt
     7141 tgccaaatta ttttttgatg cgtggaatag ccgatttaag gataaagtgc atattgtttt
     7201 tgtgatggtg acatcccatg aagagtgctg gcatattgca ttacgtatgc cttcaggaga
     7261 actttacgat ggtggaattg gccttcattg tgaagaagtt tatggagaag gttatttatt
     7321 tgaagacatg atcgaatatg accatgaacg tttggaaaaa tggtcttatg gtcttgaacg
     7381 tgactatcca cgattttgtc ctgactttaa taaagaagtg gttaattcat tgattactca
     7441 tcatctagat cgattgcaat ctcaaggaat ttgacttaat gcgttattta cctcaagaag
     7501 caattcaaaa attaagtaat gagcatcaac ccgaaccgcg tgctattttt tggctggctc
     7561 tgtttctcga gaggaaggaa tgcaagctca gatatttgat gagaatatcg ctatgaaaca
     7621 agaacaccaa ctgatttatg agctagagat ttctctttta aatccgcaaa cgagaaaatc
     7681 gattacacaa ctgaagctac tgattgctga tgaatttatt gagtatggtg cttctggttt
     7741 aatttacaat aagaacgact tacttgattc tctccctgaa gaagagccac agagctatat
     7801 ggttaatgat ttttcagttt tagaggtgtc gtccgaagtc atgttggcaa cttatagggt
     7861 cactgttgcc tcaaaaagtt ctttacgttc ttctctctgg caatacaaac acaatcgttg
     7921 gcaaatggtg tttcatcaag gcacgccttg ccaagagtaa taatttattt ttacaggtat
     7981 tatgatgatt acaatagcct atttaaaaca tcactcagat tgcataccag agcttgctaa
     8041 gatttggcat gaagtattag ggcaaatttg ggttcctgat gtgcccacct cacgtgtaga
     8101 agaaaattta cgaaatcatt taaacatcga acagttaccg ctcactatcg ttgcctttga
     8161 tgaagataaa cctgttggga tgtgctcttt acgcgtgaat gatggaatcc gccctgaatt
     8221 aaccccatgg ctaggctcat tagtggtatc accaagccat caaaaacaag gtattgcaag
     8281 acagcttatt catcgagtaa aagaagaagc ccaagcatta gggtttaaaa atctttatct
     8341 ttttgctttt gatcctactc ttccagaata ttacagtcgt cttggttggt gcatgattgg
     8401 catggatgaa tttaaaggac atcctgtaac agttatggag ataatgttgt gaagattaga
     8461 cagttgtttg atacggattg gcatctatgg aaagaaattc gtttggaagc tttgaataat
     8521 tcaccagaaa attttggctc ttcttatgaa gatgaagtgc atatgtccga tgctgatttt
     8581 caaaatggat taagtaaagg atatgtattg ggcgcatttg ttgacgattt attggtgtct
     8641 tgtgctggat tctatacact taactcaatt aaaacaaaac accgtggtgt gctttggagt
     8701 atgtatactc gtcttgaata cagagggaaa ggtattgcaa cagcattaat tcagaccctg
     8761 atccaacatg caaaaaccag cgttactcaa ttgcacctaa cctgtgtagt aagtaatttt
     8821 gtggctcaag ctttttatca aaagcaagga tttcgaattt atggtaccga acctaaagca
     8881 cttaaaatta atgatacctt ttatgatgaa tatttgatgg tattggattt caatgaggaa
     8941 cccatgaaaa aactggatac ttatcagagc ctttgtactg aagtatatga tttaagtaaa
     9001 cctaatgcac cacaagatga atactcattc tatcgaagct atgccgcaga ggttaaaggc
     9061 ttgattttag agcctatgtg cggcacaggt cgatttttac tgcctttggc tgcagaagga
     9121 tttgatgttc atggctttga tgcaagtcaa ccaatgctag aacgactgca tgcaaaagcg
     9181 aagagcaaaa atcttaagcc aaaagcctgg catggtttta ttgaagattt aaatcaatcc
     9241 gctaaatatt ctttaatttt tatacccagt ggttcatttg gtctcatcac agaaaaggtg
     9301 gatatccaaa aagccttaaa aaccatttat gaacatatgg aagataaagg actttttgta
     9361 tttgaagtag aaacacgcta tgcggttcct aaagaattag gtatatggag aggcacaaga
     9421 tggccaaaag aagatggcac aatcatccta ctcagccaat tagctatgct cgatgaggag
     9481 atttgttact ctataggtaa atatgaactg attgataaca accgtgtcat tcaaacagag
     9541 gttgaggaat acaaaattcg aatttatcaa gatctttctt tcttgctcaa tttgctcaat
     9601 gaggttggtt ttagcaatgt acgaacggtt aaagcatttg accgaaatgc atcgcctaat
     9661 gaaacagacg aaagtattgt ttttgagtgc agaaaataat tgaggtaata aatgaatatt
     9721 ctatgtccag aaccactaca aagaggagat atcgtggggc ttatttctcc ctcgagtcct
     9781 attacggaaa aagcaataga agtaggagtt catttattaa agtcgaatgg atttaatgta
     9841 aagtatgcaa agcatatgtt tgcatcagaa cgatttttag ctggtaaaga ccgtgaccgt
     9901 gcacaagatg tgatggattt ctttatagac tcagaagtga aagcaattat tgcaacccga
     9961 ggaggacaag gttcgcaacg acttttacct ttcttagatt atgaattgat taagaaaaac
    10021 ccaaaaccat tattcgggtt tagtgatact acagcgttgc aattaggcct atttaaaaac
    10081 actggcttag taagttatac agggtttact ttgaccattc atttaagtac ccaagttaaa
    10141 aaaacattat tttcttcttt gttaggccaa gagtacacaa ttcgcaaagg aataaaggtt
    10201 catggaggcg ttagccgagg tactcttctt ggtggcaatt taacccttat gactaattta
    10261 atgggcacgc cttacatgcc tacttttaaa ggatgtattt tgttggtaga agatgttggc
    10321 gtcgagccct ataatattga tggcatgttc tcacaattag atttagcagg aatttttgat
    10381 gaagtttccg gcgttatttt tggtacattt gaacaatgta aaagtaagaa gtcgaatcag
    10441 agcgatggta cggtagaaga tgtaatcaat gaatgggctt ctaaattaaa agtcccttgt
    10501 attaaagagt tttcctatgg acatggtaag caaaattgca ttcttccaat tggcaaagta
    10561 gttactttag atgcagatac tacttgcgtt acgattcaaa attaaaaatg aagaaataac
    10621 cacttgataa aactgatgag agcattggtt taatagaggc ataaatgaat acacaagaaa
    10681 tacccaaaga atatcaacac acttcaccag caggagctga agttcgattg ctgatgaata
    10741 atcatttagg tggaatggct cactgtactt tgaaggcagg cacaatttcc aaagcagttc
    10801 gtcataaaac tgtatctgaa ttttggcatg tcctttctgg tgagggtgca atttggcgta
    10861 agttgaacga tgaagagagc attacccctt taactgctgg agtcagcatc gatattccct
    10921 tgggaacaca ttttcaatat cgaagtgatg cagcagattt ggtgtttatt tgcgttacta
    10981 tgccaccttg gtcaggcagt gatgaggcaa gttatgtgga gcatggtgcc tggcctccta
    11041 cagtggaata cattaaatga ctttaaaatt atcgttttta gaaaatccaa accctgatga
    11101 tgttcaaata ttaacgaatg ggattaaggc gtacgccaaa caagcaaggg gcttcgagtc
    11161 cttagatttc tttgcatgct ttattcgtga tgcagacaat agcattgtgg gtggttgtag
    11221 tggtggaaca ctctatggag gacttcatgt cgataatctt tgggtgagtg agtccatcag
    11281 gcatcaagga tggggaacaa agcttatgca agaggcattg aagtacggta atgagaaagg
    11341 ctgtgctttt gcaacagtta atactatgga ttgggaagca atagaatttt ataaaaaatt
    11401 aggtttcgaa cttgagtttg agcggcatgg gtttcagaaa aactctattt tttatttttt
    11461 gcgtaaagaa tttcagggaa caagagacat cgggctctta aatgatgatt cagtctcgga
    11521 cttaagtcaa aatctaaaat cgtctatatc agaatctgta gtgagagaag aggttgtctc
    11581 aataagcaat gcgcctcatt ttaagtgggg acaaaattgt gatgcatggt ggttaaaaaa
    11641 tgatggtcag tttaacgttc tttatgaaac gatgccctca ggcagttttg aggtaaaaca
    11701 ttatcatcag gaaacagaac aattttttta ttgtttgcaa ggacaattgg ttattgagtt
    11761 tgaagactat gaacaggtgt tacaggaaca ggaaggaata agtattaaag cagggataac
    11821 ccataaagtg aaaaatagtt ccggaaattt ggtttctttt ttggtaattt catctcctaa
    11881 tctaatcggg gatagagtca atctggagtc ataaagtgtc ttctgaactg attatccgca
    11941 aagcattaat tgatgatgtt caacatcttc accctttaat ggagcaattg ggttatccac
    12001 aatcgtctga agagttgcaa agacgacttg agctatttac tagtgaactg cattacggtg
    12061 ttcttcttgc ggaaattgca ggaaaagttg ttggttggat tgcttggtct aagagctatc
    12121 tttttgtttc cgaaacaaca agatttcata ttgaaggcct tgtggtcgac caaagttatc
    12181 gtaatcaagg aataggtaaa aagcttatga ttactgttga ggagtttgcc aggcaattta
    12241 gtccctgtat cattgatttg acatctggac ttaggcgagc gaaagagggc tcccatgatt
    12301 tttacaaatc gcttggatat cagaatgaag gtcatatggc gaaactgtat ttaaggaaaa
    12361 tgctgtgaac atcaaagaat tacaatctgc tttaaaagtt gagacagcaa cattagatga
    12421 ttatcctacc atccaaaaca tggcacgatt ttatgtatat gaactctcgc gtgactgtgg
    12481 ttttatttca gatgattggg ctttgccttc cgatggcctg tatgaaagct tcgattttaa
    12541 aaaatacttt gaagagccga caagaaaagc ctttctgatt aaagttggta ataaattagc
    12601 tggtttcgct ctattaaatc acgaaggagt ttaccctgat actgcatgga atatgggcga
    12661 gttttttatt actgctaagt ttcaaggcaa aggtttaggt tatttagttt cctctgaatt
    12721 atggaaaatg catccaggct tatgggaggt ttctgtgatt ccagaaaaca agcgaggact
    12781 tagtttttgg cgcaaggcta tttcaagcgt tacagcgggc aattatcagg aagaaattaa
    12841 aacaatcaat tatgatacgc accaaccaca acgagttatt tttcgttttg atagtacagc
    12901 acatgaatgc agcgagcctt tagaaagcga gaacgaatat caaatccaat ttgttgatga
    12961 gcttgatgaa gccacagaaa aacgcatgac aaaaggattt attgcctatg aagctcagca
    13021 tggtattgac gtcaattatc gacgtttttc agtgaccatc tcttctgccc aaggaaaaat
    13081 ttttggtgca cttaatgcct ttactgcatt tgcagaaatt tatgtggatg atatttgggt
    13141 agacagtgct tatcgtggta aagggtatgg caagcagtta ttgcttgctc tagaacatca
    13201 cttcaaaggc cagggcttta ataatatcaa tttagtgacc agtgcttttc aagccccaga
    13261 gttttataag aagtgtggct tcatagcaga atttacgaga ataaataaaa aaaatcccaa
    13321 gttaagcaag acattttttg ttaaattttt taacgaggaa tcagaaacac aaggcactct
    13381 taaggcagct catggtgaat aaaaatcaac tggaaacaat ctgtaaaaaa ttaaagatga
    13441 accaaccttg cgacttgcct caaagagttc acggtggtct tttacataaa atgtggtatc
    13501 tgaagtgcaa tggctcgcaa tttgctgtaa aacagctcaa taaaaggctt cagttaactg
    13561 aagaagttaa atcccaatat gaattgacag aagagatagc cttccaattt tcccagtgta
    13621 ctattcccgc agttcatgct ctaaaattag aaaataatta cttaatcctt tcagaagatg
    13681 aggcctttct tgtttatcct tggataacag caaaaacatt ggataaagac gctatatctg
    13741 agatgcatgc gcttaaaata gccactcttt tggctaaaat tcatcgtatt aatcttaatg
    13801 ttccccagct aagtgctcaa gaatttgata ttcatcccaa tgaaaaaatt agtgtgttaa
    13861 tagaccagtc agcaagcaaa aatttggtgt ttaaggagca attaaaaagt aatcaacgag
    13921 ttataattga gcttaacgat atctatcaaa aagcaatacc tattttacaa agtagtagtg
    13981 tagtaagcca tggtgatttg gatcaaaaaa atgttttatg ggataaaaat gatactccat
    14041 ttctcattga ttgggagtct gcgcgaaaac ttaatcccac ttatgaaata gtcaattgtg
    14101 ctcttgattg gagtggaatt actactccgc tgtttaataa aactttattt ataaaaatgc
    14161 taaaaacata tgcgtcttct ggaataacaa taaatcaaga tcatttggaa gctgcttttt
    14221 atggtgttct tggcaattgg atcaattggc tagtatataa tataaaccgt tcttttagtt
    14281 ctgataaaga gcagcaggac attggtattg agcaggtgct tcaggtttta cctactatta
    14341 ttcaagtcaa ggcgagtatt ccagaattaa ctcaattagt aacacatgaa ttaggaataa
    14401 ccttttaact ttcgccaact ttgattacca taccgcacga aagtaactta agtttcaagg
    14461 aatgcttcat gacgaacatc aacaataata aagctgcatt agtgaccgga gccagccatg
    14521 gaattggtct tgaacttgct aaaattcttt taggtgacgg ctgggttgtc tatggaacag
    14581 ggcgggatgt gagcagctta gaggatacta aagcgctatt tcctcgtttt gttcccattc
    14641 aatcggattt tactcgcaat agtgatatcg aacaggttgc taaaactatt aatgattctg
    14701 gaataccgct tcatctttta gttcaaaatg caggaatgaa aagtcctcca cgtccgttga
    14761 ccgaatacga ttgtgacagt attgacgaag tttttcaggt taatttatta gctccaatga
    14821 aacttacggc actacttgcc gcgcagatgc ctgagaagtc tcggatttta tttgttacat
    14881 caagagcagc tactctgaaa cttaaagaaa gttcaacata ctgtgccagc aaagcaggtt
    14941 tggatgaagt aactgctatt gtgagaaaag agttagcaga gaaaaatatt ggtgtgtctt
    15001 gtattattcc aggagaagtg gatacaagaa ttcaaaaaat tttaagagaa acgacttcat
    15061 ttcatttaca taaaatgttt gatgaagctt atcagtccgg tcaattaata agtccggaaa
    15121 cttgcgcgga attccttaaa tggtttcttt gcgacttatc ttttgatgaa tttaagcaaa
    15181 gcaatatgcc cgtttccatt tatgaagagt ggcaccatcc attctggctt aaagatagga
    15241 atcagttacc tccttttcct ttc
//
LOCUS       DAXLDT010000070        15220 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXLDT010000070 DAXLDT010000000
VERSION     DAXLDT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15220
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.070"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            181..858
                     /locus_tag="ACF2SP_001754"
     CDS             181..858
                     /locus_tag="ACF2SP_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HFD2359539.1"
                     /translation="MNTLFTLISTVLLTSNITSSTPLFAKPGTDINAQVQHLSHKAPQ
                     LNKKVLKLALTAYKNASKRGAVKKPVLTVIDYSLPSNKQRMWVFDLRNERLLYNTYVA
                     HGKNSGVNVARSFSNRESSKQSSLGTYITKNTYIGHKGYSLNLQGLDRGFNDNAYNRR
                     VVIHGAWYVEPEFIKKAGRAGLSWGCPAIAQTLAKPVINTIKNGSVVFAYYPDKKFLS
                     HSGYLVA"
     gene            1284..1655
                     /locus_tag="ACF2SP_001755"
     CDS             1284..1655
                     /locus_tag="ACF2SP_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFD2359540.1"
                     /translation="MIMNQQLNATKKDKLQVIDWLIENFPNAFFKKGNQVKPLKIGIF
                     DDLIDFYERLDTPPFSKKSLREALSYYSASPAYLSCQKPDTARVDIYGNEVDVVTPEQ
                     AKYAYQRYQERYGNKKSQDLK"
     gene            complement(1683..2375)
                     /gene="ubiG"
                     /locus_tag="ACF2SP_001756"
     CDS             complement(1683..2375)
                     /gene="ubiG"
                     /locus_tag="ACF2SP_001756"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFD2359541.1"
                     /translation="MNQIESTIAIEEVHKFAQLANDWWDTNGPLRTLHDINGARFEFI
                     SEHINLKGLRVLDVGCGGGILCESMAKAGAYVSGLDAEPEAIQVAKEHAHKNQLQIDY
                     FCNPIEEYEEQGFDVITCMELLEHVQNPELVLQHCRRILKPNGLLFLSTISRTLKAYL
                     GAIIAAEYVLNLLPRQTHDYDKFIKPSELVKMARLYDLNLIDMKGLCYNPFIRKTTLV
                     SDVSINYIIVLQ"
     gene            complement(2362..3081)
                     /locus_tag="ACF2SP_001757"
     CDS             complement(2362..3081)
                     /locus_tag="ACF2SP_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFD2359542.1"
                     /translation="MYENLLVGCHEEWKEILMKALNTMDSDYLKQIQEDSHWLPGLNQ
                     LFSAFSLPLSQTQYILLGESPYPRKISANGYAFWDNSVGLLWSSTGLSKEVNRATSLR
                     NWIKTLLIARGDLHGNTSQEAIAKINKSCLVQTAEDFFKGMMKKGILLLNASLIYSKG
                     KVPYHARHWKPFMQNLFEQLVIRKPDVQLILFGKIAEKIPANKLLIGLVSEHPYNVSF
                     ITNQRVIEFFKPMDLLSYESN"
     gene            3345..5029
                     /gene="legC3"
                     /locus_tag="ACF2SP_001758"
                     /gene_synonym="ppeA"
                     /pseudo
     CDS             3345..5029
                     /gene="legC3"
                     /locus_tag="ACF2SP_001758"
                     /gene_synonym="ppeA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     LegC3/PpeA"
     gene            5165..6805
                     /gene="legC3"
                     /locus_tag="ACF2SP_001759"
     CDS             5165..6805
                     /gene="legC3"
                     /locus_tag="ACF2SP_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC3/PpeA"
                     /protein_id="HFD2359543.1"
                     /translation="MSLATYDVKQELVKLTQNFFSIDHQFSELKNLLTDKKTIPWIHN
                     QIVDQLSQIKKSDEIAMIARLEKSAYEQQMKEDDDEKNRDVEELRQDLLQMNDLTRQL
                     EILRVRKEQYERELLIYNTVPHVHTHPDTPVVHQHPSQPISAPLLNPNTHVHTHLEAT
                     LSSQDIELSVRKINKRISEITKELKILDSRNNAREIRRGDREKRLQARLNYVQKTAGV
                     VNTLSPDNQKKLLSNIEKEKKSLTQQHSSLLLKADQLNYSIFLEQFELSLQIMQGPFQ
                     EVDALQTIVKRMKGHLNYKEKAAAIQSRLDNTVLTIGENLRRLTRLNSQLSSLQLANP
                     DLTRRNERLEEQNRELLESYNSHIKTRNKLFFPTLVLSGLSLLFSIPLILTLTGIIPY
                     AIAPAVLLTLIIAPPALLLLAGLGVGIATITYAVKAYFNNSTIESNKETIESNRRQMG
                     ANQKEIYTLENQTIPNLKKELLQNEGIQNRLTDELQYFESLAEQALKQAKEVEPHTYP
                     YRPFFNSEVAVHQHPSVDNGIVTPSAPPLDPLYPTVRH"
     gene            complement(6892..8148)
                     /locus_tag="ACF2SP_001760"
     CDS             complement(6892..8148)
                     /locus_tag="ACF2SP_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFD2359544.1"
                     /translation="MFALVDCNNFYVSCERLFKPALANKPILVLSNNDGCVIARSNEV
                     KALGVQMGVPYFAVKALCKQHKVHVFSSNYTLYADMSRRVMSVLENNWPAIEIYSIDE
                     AFLDLNTLPQSEHDEFCRKLQKIVLRYTGIPVSIGIGATKTLAKLANHIAKKELRIPV
                     FNIENQYHWLAKIVVGEVWGVGRQWGKKLVQQGIYTAHDLAGMDLQLIKKLFSVVLQR
                     TVLELRGVSCGSLQDFESRKSIISSKSFGSMQTEYSALAQAISSHCARAWEKLRHQKL
                     MACHLSIFIKTNRFRFDLPQYQQTIGFKLINPTDDLRHLIRCAKLCLRKIFRPGFHYQ
                     KVGVYLGDLIEKNCLQLDLFNQVSEKELNQTERLMSVLDEINTKYGRHTLRLAAEGHV
                     KPWAMRAQSRSPCYTTSWSELPVVII"
     gene            complement(8148..8672)
                     /locus_tag="ACF2SP_001761"
     CDS             complement(8148..8672)
                     /locus_tag="ACF2SP_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFD2359545.1"
                     /translation="MSHGGARAGAGRPRGQGKYGESTKSVRIPESRIQDVMKYLQGEK
                     EFPAIPLYTSSVRAGFPSPGDDYIESMLDLNEYLIKHPASTFFVRASGESMINAGIYS
                     GDILIVDRGIEATHGKIVIVALNGELTVKRLSRQHGQVKLLAENPDFPPIDITEEYDM
                     VIWGVVTHVIHTVL"
     gene            8838..10061
                     /locus_tag="ACF2SP_001762"
     CDS             8838..10061
                     /locus_tag="ACF2SP_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFD2359546.1"
                     /translation="MVKLYKEIINSIDNHQATMVEQLHQFCEINSGTTNLTGLAIMAE
                     TLRSAFKPIADTTELITLPPLSVIDMTGNTVTQSCGDVLFISKRPHLKRRILLSGHMD
                     TVYSANNPFQKLSYLDANQINGPGVADMKGGLIVILHALVAFENTMFAEDMGWDVLIN
                     SDEEIGSPASASLFKKLAHRYQAALVYEPAMTPKGTLAKNRKGSGKLTLIATGRAAHA
                     GRAFDEGRNAICYLAEAVTAVHALNGQRNGVTINVGKIAGGEALNVVPDRAVAQLDIR
                     ISLPEDEIWVRNELNKIIRQLERPDYSLSVHGTFGRPVKRICAGTERLFHRIQKIGEA
                     FGLTIDWKDSGGCCDGNNLAHYGLPVLDTLGVRGGNIHSSDEYILLDSLSERAALSAL
                     LLIDLAQGGLEDLIK"
     gene            10058..11101
                     /locus_tag="ACF2SP_001763"
     CDS             10058..11101
                     /locus_tag="ACF2SP_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFD2359547.1"
                     /translation="MMLFRSARDSDLDAIHQLAEISGVGMTTLPKDKELLKKRLEWSC
                     ESYQRNVTNPGCEYYFFVLEEQKNKTIVGTSAIEARTGYEIPFYSYKLSKRTRVCHTL
                     NIRSDYEVLSLVNDNQGRTEICTLFLHPDYRKNNNGLLLSKGRFLFMAQYPHRFAPIV
                     IAEMRGISDDKGNSPFWDNVGANFFHMPFVEADRLTLATNKQFIADLMPRNPIYVKLL
                     SPEAQAVIGKPHQSTIPAMNILLKEGFRYNNYVDIFDAGPTIESPLLEIKTISLSRIM
                     VIKNISDEASSSNRYLLANTQIEFRATISHAVFNERENTCIISKETAKLLDVKQGDSL
                     RISPIRTEDTPPL"
     gene            11115..12602
                     /gene="astD"
                     /locus_tag="ACF2SP_001764"
     CDS             11115..12602
                     /gene="astD"
                     /locus_tag="ACF2SP_001764"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFD2359548.1"
                     /translation="MSKLQIIQSKGQYINGEWIKGNGLILESTNPASGTLLWQGNNAT
                     DEEITHACYIAHCALKSWANTSFEERARYTIAFVEQVEKNRDQLARLISLETGKPLWE
                     SQTEVSSVIGKVNLSIQAYQERTWPKQTETAEANACLRFKPHGVVVVLGAFNFPAHLS
                     NGHIVPALLAGNTVLYKPSEHTPAVAELIIQCWHNSGLPPGVINCLQGNANCGNTLLS
                     QDIQGVYFTGSYATGLRIHQQFCNRPEVILALEMGGNNPLVIDEVKDINAAVYHTMLS
                     TMITAGQRCTCARRIIIPDSQTGDLFLERFAKACKLMRIGSFDSQPEPFIGPVINHVQ
                     ALKHLHAQKQLIEIGGEIILPMSLLVEYTGLISPGIIDMTRAKNPPDEEIFAPFAQIY
                     RYNHFDEAIQLANQTRYGLSAGLLSDNKDHYLQFYQHIRAGLINWNRPTTGAASSLPF
                     GGVGCSGNHRPSAYFAADYCAYPVASMEQPLLTTPAQRLPGLVLE"
     gene            12608..13954
                     /gene="astB"
                     /locus_tag="ACF2SP_001765"
     CDS             12608..13954
                     /gene="astB"
                     /locus_tag="ACF2SP_001765"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFD2359549.1"
                     /translation="MNAYELNMDGLVGQTHHYAGLSSGNIASTNNALSISNPQAAARQ
                     GLEKMRQLYNMGLKQGLLPPHQRPNLNLLYQLGFKGSPSEQINKAYKTAPELLSACYS
                     ASSMWTANAATVSASVDTEDNKVHFTAANLISNLHRHQEADFSKKLLEFIFSNSDYFN
                     HHPLLPKSMGTSDEGAANHNRLCQSHAHSGINLFVYGKKVLGNHQFEQSPIKYPARQT
                     KEASEAIARNHLLNPERVIFACQNPLAIDQGVFHNDVISVANEHVFLVHEEAFYNQAY
                     VLDQLKEKADFPLVIIQISKEQISVSEAVDTYLFNSQLITLPDQKNMILIAPAECQAN
                     LKVKTCIDGLVADSQNPINSVYYLDLKQSMRNGGGPACLRLRVPLNDYELKAMHQGIL
                     IDNDLLDILDKWVLKYYRTELKISDLADPQLLYECLDALDELTQILKLGSIYPFQS"
     gene            13966..14691
                     /locus_tag="ACF2SP_001766"
     CDS             13966..14691
                     /locus_tag="ACF2SP_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFD2359550.1"
                     /translation="MWKTKFGKCIYISPSGYKVYQNLLYRWLTLGSEAWQTVINRYIP
                     RRPGLHYLQMLTLMARKYPNDCCLLGLGGAGIAHLLGAQKTIRSIVAVESSEEVIQIA
                     KKFFMTDLIPHFTITHQNARDFVCENKKTFQHMLLDLYDATHFPDECHSMDFFIHCKK
                     QLTNDGFLAVNLANSQEQWALVQLIKKQFPSNIIIPIKKCANIVVIVSKNDNKDLFIK
                     QVRNTNEIKKITWVKDWGYVAEY"
     gene            complement(14769..15134)
                     /locus_tag="ACF2SP_001767"
     CDS             complement(14769..15134)
                     /locus_tag="ACF2SP_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HFD2359551.1"
                     /translation="MKRNALIIMLVYLTSNPVFAKNLGKYTYEIACKNCHAPNLAKAI
                     KAPPAFDKKAWKLRFKQAKIEAKNNPSQFESAMDYLLYNVKVGKGLMHHGGLCKEADV
                     PNTDCSDEALIAAINYMRK"
BASE COUNT         4978 a         3056 c         2730 g         4456 t
ORIGIN      
        1 attgcaaata ttttgctcta ttctcttttt cagcctgtaa ttaacctcat gatatgatta
       61 atatttttac ctaatgggca acttaaaccc ttataaattt acaattaact tatttgttaa
      121 tataatcacc atcttttgta ataaccaata atggtgattt aacaagaaga aaggagcagt
      181 atgaatacat tatttactct aatctcaact gtactactaa caagtaatat cacatccagc
      241 actccattat ttgccaagcc tggaacagac attaacgccc aggtacaaca tcttagccac
      301 aaggcccctc aattaaacaa gaaagtatta aagcttgctt taacggccta taaaaatgcg
      361 agtaagagag gagcagttaa aaaacctgtc ctcacagtga ttgattattc cttgccttca
      421 aataaacaac gtatgtgggt attcgatctt cgtaacgaac gtctattata caatacgtac
      481 gtagcccatg gtaaaaattc gggagttaat gttgcacgga gtttctctaa cagagagtct
      541 agcaaacaat ccagcttagg tacctatatc acaaaaaaca cctatatcgg acataaaggt
      601 tactccttga acctccaagg cctggataga ggctttaatg ataatgcata caatagacgt
      661 gttgtgatac atggcgcatg gtatgttgaa cctgaattta ttaaaaaggc aggtcgcgct
      721 ggtttatcct ggggctgccc agctatagca caaaccttgg caaaaccggt gataaatacc
      781 attaaaaatg gttcggttgt ttttgcttat taccctgata aaaagttttt atctcactca
      841 ggttatcttg tagcataaaa aagtaagcca gggactcagc cctggctttt cgcagtcatc
      901 cgtataagaa cttgcgttct tattgtcatc ctgacaaatc agatccatct aattgcaaat
      961 ccatttgcac gactcatatc catataagtc ttgattaaaa tttagcaatt gaatggggga
     1021 agcgcaaggt gctaaatcac attttcactc aatactattt tcaactcaga aatagcctta
     1081 tatatttatt ttattaataa tcaattagtt acataactgt gtaagaatta ttgtcaggta
     1141 aacagaataa tttcttctat ggaattgaga aatttcttac atcaattaca gcttaaataa
     1201 cgtcatttta tcttttagag caatatgctg cttgattatg tcataagata tggtagttta
     1261 tttacgatga aatgtagcgg ggtatgatta tgaaccaaca actaaatgca accaagaaag
     1321 ataagcttca agtaatcgat tggcttattg aaaattttcc caatgcattt tttaaaaaag
     1381 gaaaccaggt caaaccgctc aaaattggaa tatttgatga tctaattgat ttttatgagc
     1441 gactggatac tccgcctttt agtaaaaaat cgttaagaga agcattaagc tattacagtg
     1501 cttctccagc ttacttaagc tgtcaaaaac ctgatacagc acgtgtagat atttatggta
     1561 atgaagtcga tgtagttacg ccagagcagg ccaagtatgc ttatcagcgt tatcaggagc
     1621 gctatggcaa taaaaaaagc caagatttaa aatagcgcgg aaaagccaca tttggatttg
     1681 ctttattgta ggactataat gtaatttatg cttacatcag aaaccagagt ggtcttccta
     1741 atgaaaggat tatagcatag gcctttcata tcaatgagat ttaaatcgta aaggcgtgcc
     1801 attttaacta actcactggg tttaatgaat ttatcataat catgtgtttg tctgggtaac
     1861 aaattaagca catactccgc agcaataata gccccgagat aagcttttaa cgttctgcta
     1921 atagttgata aaaataataa cccatttggt tttaatatac gcctgcaatg ttgtaaaacc
     1981 aactcagggt tttgtacgtg ttccaataac tccatacaag ttattacatc aaaaccttgc
     2041 tcctcatact cttcaatagg attgcaaaaa taatcaatct gcaactggtt tttatgagca
     2101 tgttctttgg caacttgaat agcttctggt tctgcatcta atccactcac ataagctcct
     2161 gccttggcca tagattcaca gagtatcccg ccaccgcaac cgacatccaa caccctcagc
     2221 ccctttagat taatgtgctc tgaaataaac tcaaaacgag cgccattgat atcatgcaaa
     2281 gttctgagag gaccattagt atcccaccaa tcatttgcaa gttgggcaaa tttatgtact
     2341 tcttcaatag caatagtcga ttcaatttga ttcataagac aataaatcca ttggtttaaa
     2401 gaattctata acacgttgat tagtaataaa gcttacatta tatgggtgtt ctgaaaccag
     2461 tcctatcaat aatttatttg cgggtatttt ctcagcaatt ttaccaaata agattaattg
     2521 cacatcaggc ttcctgataa ccaattgctc aaataaattt tgcataaatg gcttccaatg
     2581 acgcgcgtga tacggaacct tacctttact gtatatcagg gaagcattta ataataaaat
     2641 tccctttttc atcattcctt taaaaaaatc ttccgcagtt tgtaccagac atgatttatt
     2701 tatcttggcg atcgcttctt gcgaggtatt tccatgcaaa tccccccgag cgatcaataa
     2761 cgttttaatc cagttgcgca gtgaggttgc tcgattaact tccttactta gccctgtgga
     2821 gctccacaat aaacctacag aattatccca aaaagcatac ccattagcag aaattttgcg
     2881 tgggtatgga gactcgccta aaagaatata ttgtgtctgg ctcaaaggca ggctaaaagc
     2941 agaaaacagt tgatttagcc caggtaacca atggctatcc tcttgtattt gttttaagta
     3001 atcactatcc atggtattca atgctttcat cagaatctct ttccattcct catggcatcc
     3061 aaccaataaa ttctcataca tcttaatttt tacccttccg tcatgataat taaattttca
     3121 aattgaggta ttttgataat tacctatgtt tttaaaccag gttttcagcc actttaacac
     3181 tattcttaga ccatgagaaa ttgtcttatt ttaacatctg gaaataaacc cacgttggcc
     3241 aatagcttat ctgtcgatcg cagcgatttt cttgccatat ccatcgtggc gtgttgcctg
     3301 aatattcaaa ttacactaac atcatttaaa aatgataatc tggagtgatt atgtttttgg
     3361 ccaactgcaa tatagaggaa ttagtaacag aacatataaa acaatttcta gcggatgaag
     3421 aattatcttt ctctggctta aaagatttaa ttctgagtaa agccccaatt ccctggattc
     3481 attcctctgt aacagccgct ctgcttaaat ccagagactc cgataaaaca gaagtaaaaa
     3541 aaaccttgaa caacaatctt ataaagctca aatggctgaa gacaagattc aaaaagagca
     3601 ggatgaggcc gaagcattaa aagataaaaa attaaaggaa attttaactc gtgaattaaa
     3661 ccacatccca actcaaatca gtgaacagca aacggaactc agattacttc attataaact
     3721 agagcgactc tttgaaagcc aaactaaagt tgatgtgatt caacatcctg attcttctat
     3781 gaaaaagata aagccgtctt ctactcactc tgcgtcgatt gagcgtctgc aaagatctat
     3841 taatgaacgt gaaattaaaa ttcaatcact ttttgaacag gaagttaaca acaagataaa
     3901 attaaacgaa attgaaaagc gtgccagcat acgctcacaa catcacacta aaagggttaa
     3961 acgcgctcag gcaagaattg gatacaacag tactggagag gatattctca gtacattatc
     4021 aggaaaaaat caatccattt tgttgcgcag tattcaaaaa caacatcatg ctcttgaaaa
     4081 aaaatgcact gatctcattc aggaagctga tcagattaat tacccattat ttcttgagga
     4141 acttcagaag catttaaata aaaaaaagca taacttatct tctcaagagg tcgatgggtt
     4201 aaagtctgtt ataaaatata taaagcaaca cctggaattt gagcatgagg caatcaatac
     4261 acaatccagt ctccatatta aaaagcaatc catcagttcc cagattgtaa aattacgaga
     4321 attacaaagt aaactaaaaa ccttaaaaaa caataacccc catttgacag cggcaaatga
     4381 agaattagtt tcacgtaatt tggaattaac ggccatgaaa gagcatcatg ctaacctacg
     4441 tcatcgcttg ggcacacccg cattactttt atttggatta acttttctat tttcaattcc
     4501 tcttatttta actataagcg gagtaattcc ttttttcatt gcgcctgcgc tgctttatat
     4561 tttggtctca gcacctccaa ccatcttgct gctatcaact cttggtgttg gcatagcagc
     4621 tattgttttc agcttcaaaa tgcattccaa tgaatcagct attaaatcca atttgcagac
     4681 gatggaaaca aactctaatc aaatgagccg aaattcccaa aatttaaaat cattggaagc
     4741 gcttaccatc cccactctgg atatgcaaat caaaaaagat gaaaatttaa gagatcaatt
     4801 aatgctttct ttacaaaaac agcaacgtca agctgcccaa gcattccaga aagcaaaaga
     4861 agtagagtgc ctatcctatg ctaactcttc atttttaaat tccggcaata cccaatccag
     4921 tgacccatca ttctccacgt attcagaaga gagtgacaac gcactaaatg actcgaatga
     4981 ctctctggaa gcgctggaag aagaaacagt taatgggata gggtcgtaaa ttgtctttct
     5041 atcaataaaa tttcaatgga gcaagactga gtattaaccc cctgtaataa aactgctagt
     5101 cccaatattg ataaattagt ctttatgatc taaaatgcaa caacttgatt attgggagta
     5161 atctatgtca cttgctactt atgatgtaaa acaggaactc gtcaagctta cccagaattt
     5221 tttctctatt gatcatcaat tttctgaatt aaaaaactta ttaactgata aaaaaaccat
     5281 tccatggatt cataatcaaa tagttgatca gttatctcaa attaaaaaat cagacgaaat
     5341 agctatgatt gcgcggttag aaaaaagcgc ttacgaacaa caaatgaaag aagatgacga
     5401 tgaaaaaaat cgagatgttg aagagttacg ccaagatctt ctccaaatga atgatttgac
     5461 aagacaactc gaaatattgc gagtccgcaa agagcaatat gaaagagagt tgctcatcta
     5521 caatacagta ccccatgtcc atactcatcc ggacactccg gtagttcatc aacatccttc
     5581 tcaaccaata tctgcaccct tgctaaaccc taatactcat gtacacactc atcttgaagc
     5641 tactttaagt agccaggata ttgagctgag tgttcggaag attaacaaaa gaatttcaga
     5701 gataactaaa gaattgaaaa ttcttgactc cagaaataat gcaagagaaa ttcgcagagg
     5761 cgatagagaa aagcgccttc aagcacgcct taattatgtt caaaaaacag caggtgttgt
     5821 caacactcta tctcctgaca atcagaaaaa actgttatcc aatattgaga aagaaaaaaa
     5881 atcactcaca caacaacatt cctccttgct tctgaaagca gatcaattaa attactccat
     5941 ctttcttgag caatttgaat tgtctttgca aattatgcaa ggtccttttc aagaagtaga
     6001 cgcattgcaa acgattgtaa aaaggatgaa agggcatttg aattacaagg aaaaagcggc
     6061 agcgattcaa tcccgattag ataatacggt gcttactatt ggtgaaaatt taagaagatt
     6121 aacgcgactt aattctcaat tgagttcctt acagcttgct aatcctgatt taacaaggag
     6181 gaatgagcgt ctggaagaac aaaacagaga actccttgaa tcgtataata gccatataaa
     6241 aacacgaaat aaactgtttt tcccgactct cgttttatct ggattgtcct tgctcttttc
     6301 tattccattg atattaactc taactggaat catcccttat gctattgcac cagctgtatt
     6361 gttaactttg attattgctc cccctgcact gttattgctt gctggacttg gtgtcggtat
     6421 tgccacaatc acttatgctg tgaaagctta cttcaataat tcaacgattg aatccaataa
     6481 ggaaacaata gagagtaata ggcggcaaat gggagcaaac caaaaggaaa tttacactct
     6541 ggaaaatcaa acaattccta atcttaaaaa agagctgtta caaaatgaag gaattcaaaa
     6601 tcgcctaact gatgaattac agtattttga aagcttggct gagcaagcat taaagcaggc
     6661 gaaagaagta gagccacata cctacccata taggccattt tttaactccg aagttgctgt
     6721 tcatcaacat ccttctgtag acaatggtat agtaactcct agcgctcccc cgctcgatcc
     6781 cctctatcct accgttcgac attaatttcc agcctgggtt attaagccag gctgtcatcc
     6841 gggatgactt aggttgattt cccggatgac attatagcta tcagtttctc tttaaataat
     6901 taccacaggc aattcagacc aggaagtggt ataacaggga gaacgtgact gtgcccgcat
     6961 agcccatggc ttcacatgcc cttctgctgc caaacgtaag gtatgacggc catatttcgt
     7021 attgatttca tctaataccg acatcaatcg ctctgtctga ttcaattctt tctctgaaac
     7081 ctgattaaac aaatctaact gcaaacagtt tttttcaatt aaatctccca aatatacgcc
     7141 taccttttgg taatggaatc cgggacgaaa tattttacgc aggcacaatt tggcacaacg
     7201 aatcaaatgt cgcaaatcat cagttggatt aatcaactta aaccctatag tttgctgata
     7261 ttgtggcaaa tcgaaacgga atcgattggt tttgataaat atggaaagat ggcatgccat
     7321 caatttttga tgacgtaact tttcccaagc tctggcacaa tggctgctga tggcctgagc
     7381 aagagcagaa tactctgttt gcatagaacc aaaggatttg gaagatatta tgctttttct
     7441 tgactcaaaa tcctgtaaac tgccacatga aaccccacgc aactcaagca ctgttcgttg
     7501 caatacaaca ctaaataatt ttttaattaa ttgcaaatcc ataccagcca agtcatgagc
     7561 tgtataaatc ccctgttgta ctaacttttt accccattgc cgtcctactc cccatacctc
     7621 gccaacaact attttagcta accaatgata ttgattttca atgttaaaca caggaattct
     7681 taattccttt ttcgcaatat ggtttgcgag tttagccagg gttttggttg cccctatgcc
     7741 tatggatacg ggaataccgg tatagcgcaa aacaattttc tgtaacttgc ggcaaaactc
     7801 atcatgttca gattggggca aagtattcaa atcaagaaaa gcttcatcaa tggagtaaat
     7861 ctctattgct ggccaattgt tttcaagaac tgacataacc cggcgtgaca tatcagcata
     7921 caatgtgtaa ttggatgaga acacatgtac tttatgctgc ttacataatg ctttaactgc
     7981 aaaataaggc actcccattt gaacacccaa agcttttact tcattagatc gcgcaatcac
     8041 acaaccatca ttattagaca agaccaaaat tggtttattc gctaaggctg gtttaaataa
     8101 acgctcacac gagacataaa aattattaca atcgaccagc gcaaacatca caataccgta
     8161 tgaataacat gagtgacgac tccccaaatc accatatcgt attcctctgt aatatctatg
     8221 ggaggaaaat cagggttttc agccagcaac ttaacttgcc catgttgacg ggataaacgt
     8281 tttactgtca attcgccatt cagggcaacg attacaattt ttccatgagt agcctctatg
     8341 ccacgatcaa cgattaaaat atcaccggag taaatgccgg catttatcat ggattctcca
     8401 gaagctcgca caaaaaatgt ggatgcagga tgcttgatca ggtactcatt caaatccagc
     8461 atggattcaa tatagtcatc acccggagaa ggaaaaccag ctcttacaga actggtatat
     8521 aaaggaatcg ctgggaactc cttttctcct tgaagatatt tcatcacatc ctgtatacga
     8581 gattcaggta tacggactga tttggttgat tctccatatt taccttgccc acgagggcgc
     8641 ccagcgcctg ctcgtgcacc accatgagac atatactccc ccaaacttga atttagaatc
     8701 aaaattcaag tttaatttta aagtagccca ttgtaaagtt ccgacaataa tgagaatttc
     8761 atatgacgac tatactgaat tgacgccaat actcaaatta cgttattgtt ggggatttag
     8821 tgtaagacta aagagccatg gttaaattat ataaggaaat aatcaattca atagataatc
     8881 atcaggccac tatggtggag caactccatc aattttgtga aatcaattct ggaacaacta
     8941 acttgacagg attggccatc atggctgaaa cgttgcgttc agcattcaaa ccgatcgctg
     9001 ataccacaga attaataaca ttaccgcctt tgtctgtgat tgacatgacc ggaaacacag
     9061 ttacgcaatc ctgtggggat gtattgttca ttagcaagcg tcctcattta aaacgccgaa
     9121 ttctgctaag tgggcatatg gatacggttt attccgccaa caaccctttt caaaaattaa
     9181 gctacctgga tgctaatcag atcaatggcc cgggggttgc agatatgaaa ggcggactaa
     9241 tcgtcatctt gcacgcatta gtggcctttg aaaacaccat gtttgcggaa gatatgggtt
     9301 gggatgtatt aattaattca gatgaagaaa ttggttcacc tgcatcagct tccttattca
     9361 aaaaattggc acatcgctat caagccgctt tggtttatga accagcaatg acacctaaag
     9421 gtactttggc aaaaaaccgc aaaggcagtg gaaaattaac actgatagca acaggaaggg
     9481 ctgcccatgc tggtcgggca tttgatgaag gaagaaacgc catatgttat cttgctgaag
     9541 cagttacagc tgttcatgcc ttaaatgggc aaagaaatgg agtaacaatc aatgtaggta
     9601 aaatcgctgg tggagaagca ctgaatgttg ttcctgatag ggcagtagca caactggata
     9661 tccgaattag tctgccagaa gatgaaattt gggtaaggaa tgaattgaat aaaatcatca
     9721 gacagctaga gcgacctgat tattccctaa gtgtgcatgg cacttttgga cggcctgtga
     9781 agcgtatctg cgcagggaca gaacgacttt ttcatcggat tcaaaaaatt ggagaggcgt
     9841 ttggcttaac tattgactgg aaagacagtg gaggttgttg cgacggtaat aatttggctc
     9901 actatggctt gcctgttctg gatactctag gtgttcgggg aggcaatata cacagttcag
     9961 atgaatatat attgcttgat agcttatcag agcgcgccgc acttagcgcg cttcttctga
    10021 tagatttggc acagggtggt ttagaggatc taataaaatg atgttatttc gtagtgcccg
    10081 cgactctgac ttggatgcga tccatcaact ggcagaaatc agtggtgttg ggatgacaac
    10141 gctccctaaa gataaagaat tattaaaaaa acgcttggaa tggtcttgcg aatcctatca
    10201 aagaaatgtc actaaccctg gctgtgaata ttattttttt gtcctggaag aacaaaaaaa
    10261 taaaacaatt gtcggtacct ccgctataga agcaagaaca ggttatgaaa taccttttta
    10321 ttcttataaa ctctctaaaa gaaccagagt ctgtcacaca ctcaacatac gcagtgatta
    10381 tgaagtatta agtttggtca atgacaatca aggtcgcaca gaaatatgca ctctttttct
    10441 tcatcctgat tacagaaaaa acaacaatgg cctattgctt tcgaaaggac gctttttatt
    10501 tatggcacaa tatcctcatc gcttcgctcc aattgtcatt gccgaaatgc gagggatctc
    10561 tgacgacaaa ggcaactcgc cattctggga taatgttgga gctaattttt tccatatgcc
    10621 ttttgtagaa gcggatcgtc tgacactagc cacgaacaaa caatttatcg ccgatttaat
    10681 gcctcgcaat cctatctatg tcaaactcct ttcgccagag gcgcaagcag tcattggcaa
    10741 gccacatcaa tcgactatcc cggccatgaa tattctctta aaagaaggtt ttcgttataa
    10801 caactacgtt gatatttttg atgccggacc tactatcgaa tcaccattat tagaaattaa
    10861 aactatttct ttaagccgga tcatggttat taaaaacatc agtgatgaag cgagcagtag
    10921 caatcgctat ttactcgcca atacccaaat agagtttcga gcaacaatta gccacgctgt
    10981 ttttaatgaa cgagaaaaca cctgcattat tagcaaggaa acagcaaaat tgcttgatgt
    11041 aaaacaagga gacagtctac gtatatcacc catacgtaca gaagatactc caccattgta
    11101 acaaaggaat aatgatgtct aaattacaaa tcatacagag taaaggccaa tatattaatg
    11161 gagaatggat aaagggaaat ggtctcattc tggagtctac aaaccccgcc tccggaactt
    11221 tgctttggca aggaaataat gctacagatg aagaaattac acacgcttgt tacattgcgc
    11281 attgcgcact gaaatcctgg gctaatacca gttttgagga aagggctcgt tacactatag
    11341 catttgtgga acaagtagaa aaaaatcggg accaactggc gcgacttatt tcattagaaa
    11401 caggaaaacc attatgggaa tctcagactg aggtcagctc agtaattggc aaagtaaatt
    11461 tgtccattca ggcttatcaa gaaagaacat ggccaaaaca aactgaaact gcagaagcca
    11521 atgcctgcct ccgttttaaa ccacatggtg ttgttgtagt attaggcgcg tttaatttcc
    11581 ctgcccattt aagcaatgga cacattgtcc ctgccctcct tgcagggaac acggtattgt
    11641 ataaaccaag tgaacatact ccagcagtag cagaattgat catccaatgt tggcacaata
    11701 gcggcttacc tcccggagtt atcaattgtc tacagggtaa cgcaaattgt ggaaataccc
    11761 tattgtctca agacattcaa ggcgtctatt ttaccgggag ttatgcaaca gggctgcgta
    11821 tacaccaaca attctgtaat agacccgaag ttattttagc tttggaaatg ggcggaaata
    11881 accctttagt catcgatgaa gtgaaagaca ttaatgcagc tgtataccat acaatgcttt
    11941 caactatgat cactgcaggg caacgttgca catgtgcgag acgaattata attcctgact
    12001 cccagacagg cgatctattt ttagagcgct ttgccaaggc ttgtaaatta atgcgaattg
    12061 gatcctttga tagtcagcca gagcctttta ttgggcctgt cattaaccat gttcaagcat
    12121 taaaacatct acacgcgcag aagcaactaa tcgaaatagg gggtgaaatc attctgccga
    12181 tgtcgcttct tgttgagtac acgggactca tttctcctgg catcattgat atgactcgcg
    12241 ccaagaaccc gccagatgaa gagatttttg cgccatttgc acaaatttac cgttataatc
    12301 actttgatga ggcaattcaa ttagcgaatc agacacgcta tggtttgtca gcaggcttac
    12361 tcagcgataa taaagaccac tatctgcagt tttatcaaca tattcgtgct ggattaatca
    12421 attggaacag accaaccaca ggtgctgcca gtagccttcc atttggtggt gttggatgca
    12481 gtggaaatca tagaccaagc gcctattttg ctgctgatta ttgtgcttat cctgtagcaa
    12541 gcatggagca acctctgtta acaactccgg cgcaacgatt accaggtctg gtattggagt
    12601 aattattatg aatgcttatg aattgaatat ggatggactt gttggtcaaa ctcatcatta
    12661 cgcaggactt tcctcgggta atatagcttc tacaaataat gctttatcta tttccaaccc
    12721 tcaagctgca gcaagacaag gtttggaaaa aatgcggcaa ctttataaca tggggctaaa
    12781 gcaaggccta ttgcccccac atcaacgacc taatttgaat ttactgtatc aactgggatt
    12841 taaaggcagt cccagtgagc agataaacaa agcctataaa acagcgcccg aattgcttag
    12901 tgcctgctac tctgcctcaa gtatgtggac agctaatgca gcaactgttt ctgcaagcgt
    12961 ggatactgaa gacaacaaag tacatttcac tgctgccaat ttaatcagca atttgcatcg
    13021 tcaccaggaa gctgattttt caaaaaaatt attggaattt atcttttcca attctgatta
    13081 ttttaatcat caccccctgt tgcccaaatc aatgggtaca agcgatgagg gagcagctaa
    13141 tcataatcgc ttatgtcaaa gtcatgcgca ctcggggatt aacctctttg tatacggtaa
    13201 aaaagtgctg ggaaatcatc aattcgaaca gagcccaata aaataccctg ctcggcaaac
    13261 taaagaagcg tcagaagcca tcgctcgcaa ccatttatta aatcctgaac gagtaatttt
    13321 tgcctgtcaa aatccattag caatagatca aggtgtattt cataatgatg tcatctcagt
    13381 tgctaatgaa catgtatttc tggttcatga agaagctttt tataatcagg cttatgtttt
    13441 ggatcaatta aaagaaaaag ccgattttcc tttagtaata attcagatct caaaagaaca
    13501 aatcagcgtt agtgaagcag ttgataccta tcttttcaat tctcaattga ttaccctgcc
    13561 tgatcagaag aatatgattt taatagcccc tgctgaatgt caggcaaacc ttaaagtaaa
    13621 aacttgtatt gatggattag tcgctgattc tcaaaaccct attaactcag tgtattacct
    13681 ggatctgaaa caaagcatgc gtaatggcgg aggaccagcc tgtttacgct tgcgagttcc
    13741 tttgaatgac tacgagctta aagcaatgca ccaaggtatt ctaattgata atgatttgtt
    13801 agatatttta gataaatggg ttttaaaata ttaccgaact gaactgaaaa tatccgattt
    13861 agctgatccg caactattat atgaatgtct ggatgcattg gatgaattaa ctcagatctt
    13921 gaaattaggc tcaatatatc cctttcaatc ttgagtatcc gagtcatgtg gaaaacaaaa
    13981 ttcggtaaat gtatttatat ttcaccgtca ggatataaag tctatcaaaa tttgttgtac
    14041 cgttggctta ctttaggtag cgaagcatgg caaactgtaa ttaaccgtta tatacctcgc
    14101 aggcctggtt tacattatct gcaaatgctg acacttatgg cgcgaaaata tcctaatgat
    14161 tgttgtttat taggacttgg aggggcagga atcgctcacc tactgggtgc acaaaaaacc
    14221 atacgctcta tcgttgccgt ggaaagtagt gaagaagtga ttcaaattgc caaaaaattt
    14281 tttatgacag atttaattcc tcatttcacc attactcacc aaaacgcccg agattttgtg
    14341 tgtgaaaaca aaaagacttt tcagcatatg cttcttgatc tatatgacgc aacacatttt
    14401 cccgatgagt gtcattctat ggatttcttc attcattgta aaaagcaact aactaacgat
    14461 ggttttttag ctgttaactt agcaaattcc caagaacaat gggctctggt ccaattaatc
    14521 aagaaacaat ttccatccaa tatcattatt ccaataaaaa aatgtgccaa tattgttgtc
    14581 attgtatcca aaaatgacaa taaagacttg tttataaaac aagtcaggaa tactaatgaa
    14641 ataaaaaaaa ttacatgggt aaaagattgg ggttatgttg ctgaatatta atgttaattg
    14701 aaaggaaaca atagtttatt tacaaatgaa acccagagaa aagctggtat gtatttaaat
    14761 attttatctt attttcgcat gtaattaatc gctgcaatta atgcctcatc agaacaatcg
    14821 gtattaggga catctgcctc tttacataaa ccgccatgat gcatcaaccc tttacccacc
    14881 ttgacattgt acagcaggta atccattgcg ctttcaaatt gtgaaggatt attttttgct
    14941 tctatttttg cttgtttaaa tcgcagcttc catgcctttt tatcaaaagc tggaggggct
    15001 tttatagcct tggctaaatt aggggcatgg caatttttac aggcaatttc gtaagtatat
    15061 tttcctaaat ttttagcaaa aactggatta cttgttaaat aaactaacat tataatcaat
    15121 gcattccttt tcatcgcatc cccaaattat tattgctaaa gcaatcttag gattatatga
    15181 gatattttgc caattgaaat agttttttac tttttaactc
//
LOCUS       DAXLDT010000071        15130 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXLDT010000071 DAXLDT010000000
VERSION     DAXLDT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15130
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.071"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..375)
                     /locus_tag="ACF2SP_001768"
     CDS             complement(<1..375)
                     /locus_tag="ACF2SP_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFD2359552.1"
                     /translation="MPNSSNVLRHMENHFALTTCEDIKNIMQPLLIKHGMTVFNYYRI
                     YFDSTVIRLSTDQKWTEHYFKMNYLETLTIPESYLKKPLNYYLWLTDDCPLMLQDAAI
                     NFDMANGISIAKINHDSIEYFCF"
     gene            complement(638..1384)
                     /gene="rluB"
                     /locus_tag="ACF2SP_001769"
     CDS             complement(638..1384)
                     /gene="rluB"
                     /locus_tag="ACF2SP_001769"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFD2359553.1"
                     /translation="MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAG
                     PSDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRW
                     VQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVM
                     LDDGLGKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLT
                     MPRFLSRGQFYELTPKEVTEFLDSLPNELS"
     gene            complement(1374..1955)
                     /gene="scpB"
                     /locus_tag="ACF2SP_001770"
     CDS             complement(1374..1955)
                     /gene="scpB"
                     /locus_tag="ACF2SP_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFD2359554.1"
                     /translation="MIDNELKNIVEALVLSNNEPVTLEKIQETFDEWQRPTLEDLQAI
                     IESLKVDYSSRAFELIQVAGGFKVQTKSKYATWVARLQIEKPAKYSRALLETLAIIAY
                     KQPVTRADIEDIRGVAVNSQIIKTLMEREWIRIAGYKDVVGKPAVYTTTKEFLNYFNL
                     NYLSELPPLPEIMDTLTLHEATQPIKECTQNEQ"
     gene            complement(1957..2748)
                     /locus_tag="ACF2SP_001771"
     CDS             complement(1957..2748)
                     /locus_tag="ACF2SP_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFD2359555.1"
                     /translation="MNIDLQPAPVVKAVVDGKEITQFPEDLYIPPDALEVLLDSFTGP
                     LDLLLYLIRKQNIDILDIPMTSITNQYLQYIQLMENRRMELAAEYLLMAAMLAEIKSR
                     LLLPVTPDSEEEEEDPRMTLVRQLQAYEQIKQAAELLDGLPRQDRDNFTIQVNPDEVE
                     TIKVYPEVELSELVEAMKVLLKREEHYSKHQVSRELMSVRERMSRILFQLQHQKILEF
                     SQLYTLEEGRMGLVVSFLAILELAKQSLLIITQTQPFSPIHLQAA"
     gene            complement(2748..3962)
                     /locus_tag="ACF2SP_001772"
     CDS             complement(2748..3962)
                     /locus_tag="ACF2SP_001772"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFD2359556.1"
                     /translation="MSVLFTSNKRVVSGMRVSGRLHLGHYHGVLKNWIKLQHQYDCFF
                     FAADWHGLTTQYEDPNFIEKHLWDMIIDWLACGVNPGLCKIFIQSWVPEHAELHLLLS
                     MITPLGWLERVPTYKDQQEKLKEKDLSTYGFLGYPLLQSADVLLYHADYVPVGEDQVA
                     HIELTREIARRFNYLYGREANFEELAAAAIKKMGKKNGKIYTELRRQFQEHGIHESIK
                     TAQALLEDQPNLSIGDKERLLGYLEGSGKIILNEPQPLLTETAKMPGLDGQKMSKSYH
                     NTIMLRDEPALVEKKILTMPTDPARVKRTDPGEPEKCPVWQFHKVYSNEEVKDWVQKG
                     CRTAGIGCIDCKRPVIGAIHQELKPIQEAISDYEADLGSVKRIVAEGSEAAREEGNKT
                     LNTVREVMGLDY"
     gene            complement(4208..4828)
                     /locus_tag="ACF2SP_001773"
     CDS             complement(4208..4828)
                     /locus_tag="ACF2SP_001773"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFD2359557.1"
                     /translation="MSQFFALHPDNPQARLLRKAVSIIEEGGLIVYPTDSGYALGCSL
                     GNKSALERIRRLRQLDKHHNMTLVCRDLSQLGTYAKVSNPIFRLLKAFTPGSYTFILN
                     ATHEVPRLMLHPKRRTLGLRVPDNTITLSLLECLEAPLMSTTLILPGAQAPLSEPEAI
                     KDLLGNQIDLIIDGGNCGHEPTTVVDLTGDYPIIIREGKGDPEPFK"
     gene            complement(4825..5664)
                     /locus_tag="ACF2SP_001774"
     CDS             complement(4825..5664)
                     /locus_tag="ACF2SP_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFD2359558.1"
                     /translation="MIDLHCHSSFSDGALTPEELIKRASTLQVQCLSLTDHDTVAGYD
                     ALYGAAIHTNIEIVKGIELSTRWKKYDIHILGYQINHTTELQELIAHQNKSRIERAEK
                     IGESLHSIGIDKAYEKACLLAGHKRVGRPHFAQVLINEGKAKDMKSAFKNYLGRGKCA
                     YIPTLWISMHDAVKGISHSGGQAVIAHPLKYGLTRSKLRELITEFKEAGGVGMEVVSG
                     EMTTVQIQEMAGMCERFHLLASSGSDYHSDAQSCINLGRQRQLPIICTPIWQEWNMKQ
                     GTL"
     gene            6087..6893
                     /locus_tag="ACF2SP_001775"
     CDS             6087..6893
                     /locus_tag="ACF2SP_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFD2359559.1"
                     /translation="MIICGQSGDNFEEIKKVLKLLYPLTGEPIDTKTLRVLINEDYQT
                     AMTHHIRLSRKSISARNSKQAAYLDSINMHDITFAVGPAGTGKTYLAVSKAIECFEKG
                     EVQRLIFVRPAVEAGEKLGFLPGDLVEKVLPYLRPIYDALYEMIGFKETQKLIQTDII
                     EILPLAFMRGRTLNESFIILDEAQNTTIMQMKMFLTRMGFGSKTVITGDITQVDLPKG
                     IDSGLAHAIQLFRHFPDISIHTFTSREVVRHPLVSKIVDCYDNEMERIKK"
     gene            6890..7366
                     /gene="ybeY"
                     /locus_tag="ACF2SP_001776"
     CDS             6890..7366
                     /gene="ybeY"
                     /locus_tag="ACF2SP_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFD2359560.1"
                     /translation="MTYHIDIQNATGKLLPLSEDEITKLASLALRDHKQDAELTVRLV
                     DVEEMTYLNHTYRKKNKPTNVLAFPCSLPANIELECPLLGDVVICPEVLLAESAQFNK
                     SLHAHWSLILIHGVLHLLGYDHIKDEEASIMQMLEAKLLAELGYANPYEVEENELE"
     gene            7326..8216
                     /locus_tag="ACF2SP_001777"
     CDS             7326..8216
                     /locus_tag="ACF2SP_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFD2359561.1"
                     /translation="MLIHMKSRKMNLNKLEDGGSWFSRLKQFLQGEPQNQEELVSLLR
                     DAQIRSLINSETLAMIEGAILFSKMRVRDIMLPKNQMVCINKDDDFKHIISIVTQTGH
                     SRFPVTSENSDEVVGILHAKDLLKYQPENMESFDLLDICRQVTFVPESRRLDSLLSEF
                     RSNRNHMAIVVDEYGEVSGFVTIEDIIEQIIGDIEDEFDIDEDAYIKAHEGHCYIIKA
                     HTPIEEFNEQLNADFSDETYDTIGGIVLNKFGYLPQRGEVIIIDDFEFKVLNADSRRI
                     KLLECLDKRSPEKLNSTVKG"
     gene            8224..8904
                     /gene="cpxR"
                     /locus_tag="ACF2SP_001778"
     CDS             8224..8904
                     /gene="cpxR"
                     /locus_tag="ACF2SP_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CpxR"
                     /protein_id="HFD2359562.1"
                     /translation="MSSSILIIDDDTELTDLLTQYLEPEGFNVVCVHDGENGVKKALN
                     QVFDAIILDVMLPKLNGFEVLKAIREHLETPVLMLTARGDDIDRIVGLEIGADDYLPK
                     PCNPRELVARLRAILRRTQKIPTPKPIIEQHNIIVDCSKRHVTMGGKFLELTNAEFNI
                     LEMLIKSPGQAFSKEELTEYALGRKYTAYDRSIDVHISNLRNKLGDNPQGEPLVKTVR
                     GFGYMFNA"
     gene            8897..10264
                     /gene="cpxA"
                     /locus_tag="ACF2SP_001779"
     CDS             8897..10264
                     /gene="cpxA"
                     /locus_tag="ACF2SP_001779"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CpxA"
                     /protein_id="HFD2359563.1"
                     /translation="MRSLYWKIFVSFWLATILIIFTTAWVTSQIAQKSSQPAREEMFM
                     DSYANAAVATYESGQQSALLKWLDKIGLSRHMTLYLLTSTGEIIGAQKPPDAVKKISE
                     NLINDELPSDGIFKTGKLVVSHEILSTSGKYYRLAAVSEKPISYFVQIPWAGLTIRLT
                     LAIFISGLICYLLSRYLTQPLRSLGMAAKSIAKGNLNTRVGRFRGHSKDEIAELSNEF
                     DRMAEQLEALVHSKERLIQDISHELRSPLARLKIAIELGKKKTKHLADNEFARMEIET
                     SRLNDLIGEILDFARLEKSTTELHLSQTNIADLLAQVISDANYEFSHNSIRIQAGIIT
                     PCELNIDQRLIHRAIENIVRNASHYSPPDEKVLISSSYNKNKDQVYIDINDKGPGVPE
                     DQLEKIFNPFYRVDTSRTKKTGGYGLGLAIAARAVALHQGEIIAKNREQGGLLVRIIL
                     PADVK"
     gene            10441..11244
                     /locus_tag="ACF2SP_001780"
     CDS             10441..11244
                     /locus_tag="ACF2SP_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359564.1"
                     /translation="MSNDTKLNNASNYFFISGFVISKIKYLPIAVVSSALNLISLLFY
                     LVGYSLWFIASHFYPSQDKKMQEWYEFAQFKEQYLYAAALGLVATSISMMAIFSPIML
                     VLSGWLFFGSNIVWTIGEYNKLNNPPSHEENFSKDRQNAYVSYALSISLIGFITAVST
                     TVAFFIPVMTIPLLIVTTIVCIGVGALAFEYWIESKFGDPQPDITIDESYKQMSHDLG
                     KKITLEPNPTPEPYHGTRPLHFTPHDVKMREPSPDLQKDLSSRTCKPQY"
     gene            11277..11678
                     /gene="cdd"
                     /locus_tag="ACF2SP_001781"
     CDS             11277..11678
                     /gene="cdd"
                     /locus_tag="ACF2SP_001781"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFD2359565.1"
                     /translation="MTMDKLTSNMISEAHKVLVNAYAPYSKFSVAACICTDKNNFYTG
                     VNVENISYGLTTCAEVSAICNMVTAGERKIKSIVVLAANNLLCSPCGACRQRIHEFSS
                     SDTMVHLCNKETIFHSITINELLPLAFDFNP"
     gene            11691..12530
                     /locus_tag="ACF2SP_001782"
     CDS             11691..12530
                     /locus_tag="ACF2SP_001782"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFD2359566.1"
                     /translation="MTTSISTISYPHLAAEYIQKTCPSFKPKVGVVLGSGLGQFAEEL
                     EDTVAIEYEKLPGFPRTTVQGHGGKLILGYYGSTAVICLQGRAHTYESMENHETVKTY
                     VRTLKLLGCQYFIATNASGSLKEEVGPGELMLITDHINFQPGNPLVGPNDDEFGPRFY
                     PLDNAYDITMRNALLDIAQRHSIKLHQGVYISVLGPNYETAAEIRAFKLLGADAVGMS
                     TVPEVLVANHCGLRVAVIATITNYATGLAKTSHSHESVVKMASSAAEKLNTLIKQYIL
                     ELS"
     gene            12527..13291
                     /gene="deoC"
                     /locus_tag="ACF2SP_001783"
     CDS             12527..13291
                     /gene="deoC"
                     /locus_tag="ACF2SP_001783"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFD2359567.1"
                     /translation="MSLDKHFNDVLDKLLSNQDIISISNAQLMHCIDLTLLEADASLE
                     ALHQLQSRACQNDVAAICVLPKHLHVFKPNSKIKLATVINFPLGNDDLLVSLAEIDNA
                     IQLGVNEIDYVLPYQYYLRGNKQKALNHCDVIIQTCKKYQLTLKIILETGLFPDMESI
                     YQLSSEVIALGGRFLKTSTGKTPQGATLAAVFAIVSAVMDSTYASSCGIKVSGGIKTP
                     LQAQQYAQLAELLMGKAIHKDWFRIGASTLLEKLLS"
     gene            13345..15117
                     /locus_tag="ACF2SP_001784"
     CDS             13345..15117
                     /locus_tag="ACF2SP_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFD2359568.1"
                     /translation="MKLWIMLFNCFIVTFLITFSHAKAPVNNNKSSYCLPNQPCWPTP
                     KEWEQLNKKINGHLIKARNYLSPCIEDPQSNGCKNTLEEIKNPFYLETQPTATQSTGW
                     FDAWDSEISPYIVAAKTAQEIAHAVKFARKHHIKLVVKGTGHDYLGRSNAPDSLLIWT
                     HHMREIQFQKHFVPNGCSANHKGVPAVTAEAGTRWIEAYKEVTVNQGRYVQGGGCTSV
                     GVAGGFIQGGGFGSFSKKYGTGAGGILEAEVIIADGSILIANECQNQDLYWALKGGGG
                     GTYGIVSKITLETHELPATFGALTGSITAKTEQDFKNLIDYFIIFYRERLHNEHWGEQ
                     VILGPDNKMELALVFQGMSKTQVDRLWQPLKTWLSQKPEHYQFTINSLVLPARKFWDY
                     DYLSKNLPDHIVIDKRKNIPYPEFWWAGNQSEVSIYLTHYQSGYLPYTLFQEKFSNKL
                     SNALFEASRLTKLSLHFNKGLAGASQQAIERQRKTSMNPSVLDAAALITIIGGQQYVF
                     PKLPGHKPDLDKARFSAKQAKKAMKLISDLNPDSGTYGNEADYNLKNWQRALWGSNYE
                     KLLQIKHHYDPENLFNCHHCIGSK"
BASE COUNT         4790 a         2953 c         2759 g         4628 t
ORIGIN      
        1 aaaacaaaaa tattctatag aatcatggtt tattttagca atagatatac cattggccat
       61 gtcgaaattt attgctgcat cttggagcat caaaggacag tcatccgtta gccataaata
      121 ataattcagc ggttttttta aataggattc tggaatagtc agcgtctcca gatagttcat
      181 tttaaaataa tgctcagtcc atttttggtc tgtagagagt ctaatgacag tactatcaaa
      241 gtaaattcga taataattaa agactgtcat accatgtttt attagcaaag gttgcatgat
      301 attttttata tcttcacagg tagtcaaggc aaagtgattt tccatatgcc ttaaaacatt
      361 tgatgaatta ggcattagct ctctcttcga actcatgggc aaataatctt gcgtaggttt
      421 tcagatctgg attcagcatt ttttggaaag ccatataaat tgctccagta cttcttcaga
      481 tgttaaattt tcgttcccta aaacttcttc aactttgaat atgtttatac atgtgaggat
      541 aggattaatc aaaaataact gtccagaaaa ttatcattct tttcaagaca gttgatatag
      601 tgaatacaac gaaatccaac gtctgatttt aaatcattta agacaattcg ttgggtaatg
      661 aatcaagaaa ttcagtcact tctttggggg taagttcata aaactgccct ctggataaaa
      721 atctgggcat tgtaagcatg ccataacgga ttcttattaa tcgactaacc tcaacacctt
      781 gagactccca caaccgacgt acctctctat ttcttccttc attaagggtc acatggtacc
      841 aggaatttgt tccttctcct ccacgaaatt gaattttggt aaattttccc aaaccatcat
      901 ccaacataac ccctttcaat aagttattta aagattcctg gctgacttga ccatgaactc
      961 gtacggcata ctctctttcc aaaccatatt tgggatgcat gagttggtta gccaattcgc
     1021 cattattagt aaaaatgagc aacccggaag tatttaaatc caggcgcccc acttgcaccc
     1081 aacgcccctg tctaagatgc ggtaaattat caaatactgt tttttcaaat tttggatcat
     1141 gtcgactgga tacttcaccg accggtttat ggtaaagaag aacccggatg ttttgtttgc
     1201 cttttaaagg gttgggaatc aactttcctt tgacgctaat tttatcacta gggccagcgg
     1261 aatctcccag tttagctggt ttgccattaa cctgcaccca accattatca atccaccgct
     1321 ccatttcacg acgagatcca agtccggctt gacttaatat tttctgtaat ctttcactgc
     1381 tcattttgcg tacactcttt tataggttgg gttgcctcgt gcagggttaa tgtatccatt
     1441 atttcaggta agggaggaag ttcacttaag taatttaagt taaagtaatt taaaaattct
     1501 tttgtcgttg tataaacagc cggcttccct actacatctt tatagcctgc tatacgaatc
     1561 cattccctct ccattaacgt ttttataatc tggctgttaa cggcaactcc tctaatgtct
     1621 tcaatatcag cacgcgtaac aggttgctta taagcaatta ttgccaaagt ttcaagcagt
     1681 gcgcgcgaat actttgctgg cttctcgatt tggagacgag caacccaagt ggcatatttg
     1741 gattttgttt gtaccttaaa accacctgca acctggataa gctcaaaagc acgggaagaa
     1801 taatctacct ttaagctctc tatgatagcc tgcaaatctt ctaatgttgg tctttgccac
     1861 tcatcaaagg tttcttgtat tttttctaaa gtaacgggct cattattgga taataccaaa
     1921 gcttcaacaa tattttttaa ttcattgtca atcatttcat gcagcctgca aatgtatagg
     1981 tgaaaatggc tgagtttgtg taataatcaa taaagactgt ttagccagtt caagaatagc
     2041 taaaaaactc acaaccaatc ccatcctgcc ctcttcaaga gtatataatt ggctgaattc
     2101 caaaattttt tgatgctgca attgaaagag aatacgactc attcgttcac gaacagacat
     2161 taactccctg gaaacttgat gtttggaata atgctcttca cgtttcagca aaactttcat
     2221 agcctcgact aattcagata actcaacttc aggataaact ttaattgttt caacctcatc
     2281 tggatttacc tgaattgtaa aattatctct gtcttgtctt ggcaatccat ccaataattc
     2341 agccgcttgt tttatttgct cataagcttg caactgtctt accagagtca tacgtggatc
     2401 ttcttcctcc tcctctgaat ctggtgtgac tggcaataat agacgtgact tgatttcagc
     2461 taacatggca gccattaata aatactcggc agccagctcc attcttcgat tttccatgag
     2521 ctggatatat tgtaaatact ggttggttat acttgtcatg ggaatatcaa gaatatcaat
     2581 attttgcttg cggattaaat acagcaataa atctaatggc ccggtaaaag aatccagtaa
     2641 cacctccaat gcatcaggag gaatatacaa atcctctgga aactgagtaa tttccttacc
     2701 atccaccacc gctttgacta caggcgcagg ctgcaaatcg atattcatta ataatcaagc
     2761 cccatgactt ctctgactgt atttaaagtc ttgtttcctt cttctctggc cgcttcactt
     2821 ccttctgcta caatacgttt taccgaacct aaatcagctt cataatctga aattgcttcc
     2881 tgtattggtt taagttcttg atggatagca ccaatcactg gcctcttaca gtcaatacaa
     2941 ccaattcctg ctgttcggca acctttttgc acccaatctt ttacttcctc attagaatac
     3001 actttatgga attgccaaac aggacatttt tcaggctctc ctggatctgt acgtttaaca
     3061 cgcgccggat ccgtgggcat agtcagaatt ttcttttcaa ctaaagctgg ctcatctctt
     3121 agcatgatcg tattatggta agatttggac attttctgcc catcaagacc aggcattttt
     3181 gcggtttccg taagcaaagg ctgtggttcg tttaatataa ttttgccact tccttccaaa
     3241 tatcccaaca accgctcttt atctccaatc gacaaattag gctgatcttc taaaagcgct
     3301 tgtgctgtct taatcgattc gtggatgcca tgctcctgaa attgtctgcg taattcggta
     3361 taaattttcc cattcttttt acccatcttt ttgattgctg cagcagccag ctcctcaaaa
     3421 ttagcctctc taccataaag atagttaaat cttcgggcta tttctcgtgt taactcaata
     3481 tgagccactt ggtcttcacc cactggaaca taatccgcgt ggtataaaag tacatcagca
     3541 ctttgtagta aaggatatcc caaaaaacca taagttgata aatctttttc ttttaatttc
     3601 tcttgctgat ctttataagt aggaactcgc tccaaccaac ctaaaggagt gatcatggac
     3661 aaaagtaaat gtaattcagc atgctctgga acccaggatt gaataaatat cttacataag
     3721 cctggattaa ctccacaagc tagccaatca ataatcatat cccaaagatg tttttcaata
     3781 aaattggggt cttcatattg tgttgttagg ccatgccaat ctgctgcaaa aaagaaacaa
     3841 tcgtattgat gttgtaattt tatccaattt tttaaaacac cgtgataatg acctaaatgc
     3901 aatcttccac tgacccgcat tccagaaacg actcgcttat tggaagtaaa cagtactgac
     3961 atcaatttcc tcttcttatt ataaattaat caattatttc aaatacaaaa atttctttat
     4021 tccctgaatt ctaacctgat gcttgattca atcatatttt atccaaattt tatcaagatt
     4081 agagacatga gattaataat caaaagctaa ctggacaaaa taaaattcaa tggcgtcaac
     4141 tctagtctgg taggagcaca taaaattttc tttcactaaa tactaacaaa accgcgactg
     4201 tattccatta cttaaagggt tcaggatctc cttttccctc tcttataatt atcggataat
     4261 ctccagttaa atccactact gtagtgggtt catggccaca atttccacca tcaattatta
     4321 aatcaatttg gttgcctaat aaatctttaa ttgcttcggg ctcgcttaat ggcgcttgag
     4381 cccctggtaa aattaaagtg gtgctcatta aaggggcttc cagacactcc aataaagaca
     4441 aagtaatcgt gttatcaggt actcttaacc ccagtgttct tcgttttggg tggagcatca
     4501 aacgaggcac ttcatgtgta gcattaagaa taaaggtata agagcctgga gtaaaagcct
     4561 tcaataatct aaaaattgga ttagaaacct ttgcataagt acctaactgg gataagtcac
     4621 gacaaaccag ggtcatatta tgatgcttat ccaattgacg taaacggcga atacgctcca
     4681 gggctgattt attgcccaaa ctacagccca aagcatagcc agaatctgtc gggtaaacga
     4741 taagcccccc ttcttcaata attgaaactg cttttctcaa caagcgtgct tgaggattat
     4801 ctggatgtaa agcaaaaaac tgactcataa agtcccctgt ttcatgttcc attcttgcca
     4861 aataggcgtg catattattg gtaattgtcg ctgacgcccc agatttatac aagattgagc
     4921 gtcactatgg taatctgaac ctgatgaggc aagcaaatga aatcgctcac acatcccggc
     4981 catttcttga atttgtacag ttgtcatttc tccagaaacg acttccatgc caacaccccc
     5041 agcctcttta aactccgtga ttaactcacg caattttgaa cgcgtcaatc catatttaag
     5101 tggatgagca attacagcct gtccaccaga atgagatata cctttgaccg catcgtgcat
     5161 gctaatccat aaggtaggta tataggcgca cttgcctctc ccgaggtaat ttttaaaagc
     5221 cgacttcata tcttttgcct tcccttcatt aatcagtact tgggcaaaat ggggtcgacc
     5281 cacccgtttg tggcctgcca aaagacaggc tttttcataa gctttgtcaa taccaatcga
     5341 atgcaaggat tctccaattt tctcggcgcg ctcaattcga cttttatttt gatgagcaat
     5401 taactcttgt aattctgttg tgtggttaat ttggtaacca agtatatgaa tgtcatactt
     5461 cttccaacga gtactgagtt ctatcccttt aactatttca atatttgtat ggattgcagc
     5521 accatataaa gcgtcatatc ctgcaacagt atcatgatca gtaagagata ggcattgaac
     5581 ttgtaaagta ctcgctcttt ttattagttc ttctggagtc agtgcaccgt ctgaaaaaga
     5641 actatgacaa tgaagatcga tcataaattg cagctaattt aaaaaaatcg aagtatagca
     5701 taattaacgt gagttcgaca taaaaggctt ttatgacttt tatgtcgcta aacgcacgtt
     5761 atcccgtaaa aaagcaaagc tttttatacg ggatccattc aaaataccca attctatata
     5821 agaaaaacca ggtaaaaata tgatgttgta aagactaaac tatccttttc tttgaacaac
     5881 ttgcacaaca tagtactttt gcactattct ttaagtatgg tcattaatct ttaataaaaa
     5941 ctttgagtag tacattaaac agtgaaataa tcaaattacc acctcttaat accagggaac
     6001 tcgcaaacct gtgtggggtt ttgcacagta acttaaaaat tttagaacag cactttaagg
     6061 tttcaataaa gatcaaaaat catagcatga taatatgtgg ccaatcaggt gacaattttg
     6121 aagagataaa aaaagtatta aaattattgt atcctttaac tggcgaacct atagatacca
     6181 aaactctacg agtcttaatc aatgaggatt atcaaacagc aatgactcac catatcagat
     6241 tatctcgaaa atcaatttct gcacgtaaca gcaaacaagc cgcatatctg gattctatta
     6301 atatgcatga tattacattt gccgtagggc cggctgggac tggtaaaacc tacctggctg
     6361 tttccaaagc aatagaatgt tttgaaaaag gcgaagtaca aagacttatt tttgtaaggc
     6421 ctgctgtaga agcaggtgaa aaactcggtt ttcttcccgg cgatctggta gaaaaagtct
     6481 taccttattt aaggcctatc tatgacgcac tgtatgaaat gataggcttt aaagaaactc
     6541 aaaagctgat tcaaacagat atcattgaaa ttctcccatt agctttcatg aggggcagaa
     6601 ctttaaatga atcttttatt attctggatg aggctcaaaa caccactatc atgcaaatga
     6661 aaatgttttt gacacgcatg ggctttggct ctaaaacagt catcacaggt gatatcactc
     6721 aagtcgactt gcctaaaggt attgactcgg gccttgcgca tgccattcag ttattcagac
     6781 attttcctga tatcagcatt catacattta ccagtcgtga agtcgtcaga catcctttgg
     6841 tttcaaaaat cgtagattgc tatgataatg agatggaaag gattaaaaaa tgacctatca
     6901 cattgacata caaaatgcta cagggaagtt attgccctta agtgaagatg aaatcaccaa
     6961 gctggcatca ctagctctaa gagatcataa gcaggatgct gagctaacag ttcgtctggt
     7021 tgatgttgaa gaaatgactt atcttaacca tacctataga aagaagaaca aaccaaccaa
     7081 tgttcttgca tttccttgct cactaccggc gaacattgaa ttggaatgtc ctcttttggg
     7141 tgacgttgtt atttgccctg aagtgttgct tgcagaaagt gctcaattca ataaatcgct
     7201 acatgcacat tggtctttaa tcctgattca tggagtgttg catttattag gttatgatca
     7261 tataaaagat gaagaagcct ctattatgca aatgctcgaa gctaagctac tagctgaatt
     7321 aggttatgct aatccatatg aagtcgagga aaatgaactt gaataaattg gaagatggtg
     7381 ggtcgtggtt ttctcgttta aaacagttct tacaaggaga gccacaaaat caggaagaat
     7441 tggtcagttt attaagagac gcacaaatac gctccctgat taattcagag accctcgcaa
     7501 tgattgaggg agctatcctt ttctcaaaaa tgcgtgttag agacataatg ctgcctaaaa
     7561 atcaaatggt ttgcattaat aaggatgatg attttaagca tattatatct attgtaactc
     7621 aaacgggaca ttcacgtttt cctgtaacga gtgaaaattc tgatgaagtg gttggcattt
     7681 tgcacgcaaa agacttgtta aaatatcaac ctgaaaatat ggaatcattt gatttattgg
     7741 acatctgccg ccaggtaact tttgtacctg aaagcagacg tcttgatagt ctgcttagtg
     7801 aatttcgaag taatagaaat catatggcta ttgttgttga tgaatatgga gaggtgtcag
     7861 gtttcgttac catcgaagat atcattgaac aaataatcgg tgacatagaa gatgagtttg
     7921 atatcgatga agacgcctat atcaaagcac atgaaggaca ctgctatatc atcaaagccc
     7981 atactccaat agaagaattt aacgaacagc tcaacgctga ttttagtgac gaaacctatg
     8041 atacaattgg tggcatagta ttaaataaat tcggctattt acctcaacga ggtgaagtaa
     8101 taatcataga tgattttgaa tttaaagttc ttaatgccga ttcgagaaga ataaagttat
     8161 tagaatgtct cgataaaaga agtcccgaga aactaaattc tactgttaaa ggataagtaa
     8221 ttcatgagca gctctattct cattattgat gatgacacag aactaacaga tttattaact
     8281 caatatttag agccagaagg ctttaatgta gtatgcgttc atgatggaga aaatggcgtt
     8341 aaaaaagctt taaatcaggt atttgatgcc attatactcg acgtcatgct gcctaagctt
     8401 aatggttttg aagtattaaa agcaataaga gagcatttgg aaacacctgt attgatgtta
     8461 actgctcgtg gtgatgatat cgatcgcatt gttgggttag aaattggtgc agatgattac
     8521 ttacctaaac cctgtaatcc aagagagtta gttgcacgtc tgcgtgctat tttaagacgc
     8581 actcaaaaaa tcccaactcc caaaccaatt attgaacaac ataatatcat tgtggattgc
     8641 tccaaacgcc acgtgactat gggaggaaaa ttccttgaat taaccaatgc ggaatttaat
     8701 attttggaga tgttaatcaa atcaccagga caagcatttt ccaaagagga actcaccgaa
     8761 tatgctctgg gaagaaaata cactgcttat gaccgcagta tcgatgttca catcagcaat
     8821 ttgcgaaata aattaggcga taatccccag ggggaacctt tagtcaaaac tgtccgcggg
     8881 tttgggtaca tgtttaatgc gtagcctgta ctggaaaata tttgtttctt tttggcttgc
     8941 caccatttta attattttta ctacggcatg ggttaccagc caaatcgcac aaaaatcatc
     9001 acaaccagct cgagaagaaa tgttcatgga cagctatgcc aatgcagctg tcgcaaccta
     9061 tgagtcaggc caacaatcag ctcttttgaa atggcttgat aaaataggcc tttctcgtca
     9121 tatgacactg tatttactaa ccagcactgg cgaaatcatc ggcgcacaga agccacctga
     9181 cgctgtaaaa aaaatttcag aaaatttaat aaatgatgaa ctcccaagtg atggcatatt
     9241 taaaacaggg aaattagtcg ttagccatga gatcctatca acttctggaa aatattaccg
     9301 gctagcagcg gtcagtgaaa aaccaatctc ttattttgtg caaattccat gggctggact
     9361 gacaatacgc ttaactctgg ccatattcat cagtgggctt atctgctatt tactctcacg
     9421 ttatttaact caacctttac gctcattagg catggcagca aaatctatcg ctaaaggaaa
     9481 tttaaatacc cgggttggtc gcttcagagg tcatagcaaa gatgagattg cggagttaag
     9541 taatgaattc gatcgaatgg ccgagcaatt agaagcgctt gttcattcca aagaacgatt
     9601 aatacaggat atttcccacg aattacgctc tccattggct cgacttaaaa tcgccataga
     9661 attggggaaa aagaaaacca agcatttggc tgataatgaa tttgcccgca tggaaattga
     9721 aacatccaga cttaatgatt tgataggcga aattctcgat tttgctcgtt tagaaaaatc
     9781 gacaacagaa cttcatttat cccaaacaaa tatcgcggat ttgcttgcac aggttataag
     9841 tgatgccaat tatgagttca gtcacaattc tataagaata caagctggaa taattacacc
     9901 ttgtgaatta aatattgacc aacgactaat acacagagct atagaaaata ttgttcgtaa
     9961 tgcatcacac tattctcctc cagatgaaaa ggtccttatt tcatcaagct ataataaaaa
    10021 caaagatcaa gtttatattg atattaatga taaaggacct ggggtgcccg aagatcaact
    10081 ggaaaaaata tttaatcctt tctaccgcgt ggatacctcc agaacaaaaa aaaccggtgg
    10141 ctatggcctt gggctcgcaa ttgcagccag ggctgtagca ttacaccaag gagaaattat
    10201 tgctaaaaat agagagcaag gtggtttgct tgttcgtatc attttacctg ctgatgttaa
    10261 ataggatttc tatatatcga gttcctcctt attgactaaa attgattttt gacttgcatt
    10321 ttattatcta attccgtata catacaaata attatacatt ttgatcataa tccaggttta
    10381 ctggataaaa cctatagatt taagtaaaat gaatctaaaa aataattgga atgccatttt
    10441 gtgagcaatg atacaaaatt aaataacgca agtaattatt tctttatatc tggatttgta
    10501 atttcaaaaa ttaaatacct tcccattgcc gttgtttctt ctgccctgaa tctgatttca
    10561 cttttgtttt acctggttgg ctatagtttg tggtttattg caagtcattt ttatccaagc
    10621 caagataaaa agatgcaaga gtggtatgaa tttgctcaat ttaaagaaca atatctttac
    10681 gccgcagctc tagggctcgt cgcaacatct atcagtatga tggcaatctt ttccccaata
    10741 atgcttgttc tttcaggatg gctgtttttt ggcagtaata ttgtttggac aattggtgag
    10801 tacaacaaat taaataatcc acccagccat gaagaaaatt tttccaaaga ccgtcaaaat
    10861 gcctatgtgt cttatgcttt atccataagt ttaataggat ttattacagc agtctcaact
    10921 actgtcgctt tctttattcc agtaatgact atacccttac ttatagttac aactattgta
    10981 tgtattgggg taggagcgtt agcgtttgaa tattggatcg aatcgaaatt tggcgaccct
    11041 cagcctgata tcactatcga tgaaagctac aaacaaatga gccatgattt ggggaaaaaa
    11101 attactttgg agcccaatcc taccccagaa ccttatcatg gaaccaggcc acttcacttt
    11161 acgccacatg atgtaaaaat gagagaaccg tctccagatc ttcaaaagga tttatcctct
    11221 cgtacttgta aaccacagta ttaatttagt atattctgca cgccaatgat gagggtgtga
    11281 caatggataa attaacttcg aacatgatta gcgaagcgca taaggtacta gtcaacgctt
    11341 atgcccccta ttccaaattt tcagtagctg cctgtatatg cactgataaa aataattttt
    11401 atacaggggt gaatgtagaa aatatttcct atggactaac aacctgcgct gaagtttcag
    11461 cgatttgtaa tatggttacc gcaggtgagc gaaaaattaa aagcattgtg gtattagctg
    11521 caaacaattt gctatgctct ccttgtggcg catgccgaca aagaattcat gagttttcat
    11581 cctctgatac catggtacat ctctgtaata aagaaacaat atttcatagt attacaatta
    11641 atgaattact acccttggct tttgatttta acccttaatc ggacgatttt atgacgactt
    11701 caatatctac tatcagttac ccacatttag cagcagaata tattcaaaaa acatgtccat
    11761 catttaaacc aaaagtgggt gtagtattag gctcagggtt aggtcaattt gccgaagagt
    11821 tagaagatac agttgctatt gaatatgaaa aactcccagg atttcccaga actacagtac
    11881 aggggcatgg aggaaaatta attttaggat attatggatc tactgcggtt atttgcttac
    11941 aaggcagagc ccatacttac gaaagcatgg aaaatcatga aacagtaaaa acctatgtaa
    12001 gaactttaaa gctattaggt tgccagtatt ttatagcaac aaatgcttct ggttcactta
    12061 aagaagaagt tggaccgggt gaattaatgc taattaccga tcacatcaat tttcaacccg
    12121 gaaatcccct ggttggtcca aatgatgatg agtttggacc caggttttat cctttagata
    12181 atgcttatga cattaccatg cgtaatgcat tattggatat tgctcaaaga cactcgatta
    12241 aactacacca gggggtttat atctctgtct tgggaccaaa ctatgaaaca gctgctgaaa
    12301 ttcgtgcctt taaattgctg ggagctgatg cagtggggat gtcaactgtg cctgaagttt
    12361 tagtcgccaa tcattgtggc ttgcgtgtcg ctgtcattgc aaccataaca aattatgcaa
    12421 ctggccttgc aaaaaccagt catagccatg aatccgttgt caaaatggct tccagtgccg
    12481 ctgaaaaact taatactttg attaaacagt atattttgga attatcgtga gtttggataa
    12541 gcattttaat gatgtcttgg acaagctcct gtcaaaccag gatattatat ctatttcgaa
    12601 tgctcaattg atgcattgca ttgatttgac tctgctagag gcggatgctt ctcttgaagc
    12661 attgcatcaa ttacagagcc gagcctgtca aaatgatgtt gcagctattt gcgtactacc
    12721 caaacatctt catgttttta agcctaactc caaaattaaa ttagcaactg ttatcaattt
    12781 tcctcttggg aatgatgatt tgctggtttc cttggcagag atagataatg ccattcaact
    12841 gggtgttaat gaaatagatt atgtactacc ctatcagtat tacttaagag gaaataagca
    12901 aaaagcgcta aatcattgtg atgtcattat tcaaacctgc aaaaaatatc aacttacctt
    12961 aaaaataatt ttagaaactg gcctttttcc agatatggaa agcatttatc agcttagctc
    13021 agaagtcatt gctcttggtg ggcggtttct caaaacttcg actggaaaaa cccctcaggg
    13081 tgcgactctg gcagcagtat ttgctatagt cagtgctgtt atggattcaa cttatgcatc
    13141 ctcttgtgga attaaggttt caggagggat taaaacacct ctccaggctc aacaatatgc
    13201 tcaactggct gaattactta tgggcaaagc aattcataag gattggtttc gtattggagc
    13261 cagtactctg cttgagaaat tattgtcatg aacgcacaat ctatgtgtta atatgtcagc
    13321 ctgcaaacag ggagaggtct tgcaatgaaa ttatggataa tgctatttaa ctgctttatt
    13381 gttacctttt taataacatt ctcccacgca aaagcgccag taaataacaa taaatcatct
    13441 tattgcctcc ctaaccaacc atgctggcca acccccaaag aatgggagca actaaacaaa
    13501 aaaataaatg gccatttaat aaaagccagg aactatctgt caccttgcat tgaagatccc
    13561 caaagtaatg gttgtaaaaa taccctggag gaaataaaaa atccctttta tttggaaaca
    13621 caacctaccg caacccagtc caccggctgg tttgatgcat gggattcaga aataagccca
    13681 tacatagttg cagcaaaaac agcgcaggaa attgctcatg cagtaaaatt tgctagaaaa
    13741 catcatatca aactagttgt caaaggcact ggtcatgact atcttggaag atccaatgct
    13801 cctgattctt tgctgatttg gacacatcac atgcgagaaa tacaattcca gaaacatttc
    13861 gtcccaaatg gttgttctgc aaatcataag ggtgttcctg cggtaactgc tgaagcaggt
    13921 accagatgga tagaggctta taaagaggtt actgtaaacc agggtcgcta tgttcagggt
    13981 ggaggctgta cttccgttgg agttgctggg ggatttattc aaggcggggg atttgggagt
    14041 ttttctaaaa aatatggaac cggtgcaggg ggaattttag aagcagaagt aattatcgct
    14101 gatggttcaa ttttgattgc caatgaatgc caaaaccagg atttatattg ggcattgaaa
    14161 ggaggtggcg gcggaactta tggtattgta agtaaaatca cattggaaac gcatgaattg
    14221 ccagcaacat ttggagcatt aacaggaagt attacagcaa aaacagagca ggattttaag
    14281 aatctcatag attattttat tattttttac agggaaagac ttcataatga acattggggg
    14341 gaacaagtca tattaggccc tgataacaaa atggagttgg cattggtttt tcaagggatg
    14401 agcaaaactc aggttgatag attatggcaa ccattaaaaa cttggttatc ccaaaaacct
    14461 gaacattatc aatttacaat aaacagcctg gtactgcctg caagaaaatt ctgggattat
    14521 gattatctaa gcaaaaatct acctgatcat attgtaattg ataagcgaaa aaatatccct
    14581 tatccagagt tttggtgggc tggtaaccag tcagaagttt ctatctattt aactcactac
    14641 caatccggtt atttgcccta tacgctcttt caagaaaaat tttcgaataa actatctaac
    14701 gctttgtttg aagcatctcg cctgaccaaa ctgtctttgc attttaacaa aggcttggca
    14761 ggggcgtctc agcaagcaat cgagagacaa agaaaaacat caatgaaccc ttccgtattg
    14821 gatgcagcag ccttgataac aataattggg gggcaacaat atgtctttcc caagttgcct
    14881 gggcacaaac ctgatttaga taaagcacgg ttttcagcta agcaagcaaa aaaagcaatg
    14941 aagctaatat ctgatctgaa tcctgattca ggcacttatg gaaatgaggc cgattacaat
    15001 ttaaaaaatt ggcaaagagc tttatgggga tccaattatg agaagttatt acaaattaaa
    15061 caccattatg atcctgaaaa cctgtttaat tgccaccatt gtattggaag taaataaaaa
    15121 ttaattaata
//
LOCUS       DAXLDT010000072        14715 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXLDT010000072 DAXLDT010000000
VERSION     DAXLDT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14715
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.072"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..246
                     /locus_tag="ACF2SP_001785"
     CDS             <1..246
                     /locus_tag="ACF2SP_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFD2359569.1"
                     /translation="SLIAKNNGYSCLALHAGSLLCPRSKDNLNSYYVIEANQKKCRIT
                     WRVFDQEHFTNRSIYFIDFNKKNASFEHINPVKDDTR"
     gene            complement(429..2711)
                     /gene="legC4"
                     /locus_tag="ACF2SP_001786"
     CDS             complement(429..2711)
                     /gene="legC4"
                     /locus_tag="ACF2SP_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC4"
                     /protein_id="HFD2359570.1"
                     /translation="MFPYVSLAHFNELFEIMKPNLFYFVLTQILAESPNFKLLDEVRP
                     VHDITLKDKKYYRLELPLANIAAEGFRLHDAHISLYEKTDPHNPNLGLSHFTAIFHDQ
                     NGQTYRMHLFLNRFDALACPATWELLDDDEQYVKVSPPENLEYFIHSVWQLGLPCLQL
                     LRKKQKEIEAKLLADYHRLNEETTSLSKDLDKNRAVYLEKLDELITTTKSLSQISESN
                     HWLREAIYLGKTHKYVSAMPEPILEPVKKSDEEHKKDDKAEVVALSPKQGPKEPKISR
                     GNIGLAQSTLFSKSVKAKRPVDVKMERDISKIKKLFAQLLKTEDKKIKAVLLIDLHWK
                     IKDINLETEELLTKEQTQALNEIEGRANKEAKSLLERALFAGEFEYAQSLSPYYPLIN
                     NDVMVLALTQRKADLLSFLVTKVGLPINSYPIKTKVKTYSNAVEYCFSEHSESLVDCF
                     SVLIKNGASLMQPVGLHKLPLAHLLLSEIPRHPLYAALEQNKSLTLNNKQFYAHLINV
                     LKSCLLSDDIEGDQKIALEESIARYEHLKTNVKNSSSLFSPQNQALVDEISDITKKLL
                     PDAVAEAIESDEEISREKAIADKEYKELVRKVKAFYHKTGKNFAFQSLINSANQELKK
                     ELLEIDFNIDISFSELKESILENLSNERLMFSYCSELIDVQTTIMQISQGASRKNKNL
                     KKLNARHAELIELLTDLSQNTPQSMIKEANEFQDSFSQFQDLLNGLNQLEGSFSMLAG
                     VLNQFIGGSTSESESFGMKL"
     gene            complement(3103..3741)
                     /locus_tag="ACF2SP_001787"
     CDS             complement(3103..3741)
                     /locus_tag="ACF2SP_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359571.1"
                     /translation="MKYIFLFIGLIVAGINPVFASVLPSSKDAQLCTNAYNKLWHIFD
                     SVKADHRGDPAAQATIFNVEFPKVLAEDFSLTINNLPVEPNSSETITVNVQGLPQVIS
                     VASEQITSWGQHHIAGPFSFKLLSESLPYKTRVYLMTTRDIDYTNSPENNGCAAFLAQ
                     KNMVCTVKNTGLTTRKAMLNSMNSHVVTTYIMPAPNCSLWIAPPVEDFPTEA"
     gene            complement(3987..5276)
                     /locus_tag="ACF2SP_001788"
     CDS             complement(3987..5276)
                     /locus_tag="ACF2SP_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFD2359572.1"
                     /translation="MSFYIQLASLSIIILYVLLLFHNVDPLVATAVCVGLGFLWNIST
                     PIDIGNAMADALGSFMALVGFIIMLGRGLGEVLTHTQVSHTLVHQIVYGVGVNTQRRA
                     KIGIVLSSFIIVGLLGTLAGGLAILAPSLRPIAGSVGLSRPSLAVLMQASAEEALILG
                     PFAPPVVTLLGVTGLSYGTVFLYASLPIAVVTLITTWFMASRLQKLYINEPCENENST
                     EPFTPNRQQKRATLIFLISFLFCVVYGLFTQAKTSYVIFVMLFLALITGLSCRLGLKQ
                     IFNLLFEGMQKSLHLFFLFILFDPFMALIHQAGGFNALTELLTPLINLGGKPVLSILI
                     GFTGAFGMPGAAEATIKMLHQLFSPSVIQMQLPLITFALCMLFATRVTNYAYPGANMF
                     AAMGFAGSENVKAMIQNGLVVTGVQVAFLIVYSLFIP"
     gene            complement(5276..6379)
                     /locus_tag="ACF2SP_001789"
     CDS             complement(5276..6379)
                     /locus_tag="ACF2SP_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HFD2359573.1"
                     /translation="MNIKEIHIANLTIPLRRPFITAVRRTEYVDDVVVLIKTDCGKIG
                     YGSAASTPAITGDSKESIIAAIKSALGPPLIGRDISEFNLLLQMNDRAIEGNSSAKAA
                     IDIALHDLFAQYCGLPLYKQLGGNTNCINSCITISVKEVEEMVSDAIDLIHRGHKTLK
                     IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPVVE
                     QPVNARNLPDLKTISDQVGCAIIADEACFSPEDALNIAKINACDGVNIKLMKSGGIEK
                     AQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYADLDPIFLIRENYILG
                     GAQNLSNKIILADKPGLGIEGITQGLNLIGVIR"
     gene            6682..7935
                     /locus_tag="ACF2SP_001790"
     CDS             6682..7935
                     /locus_tag="ACF2SP_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HFD2359574.1"
                     /translation="MLFKLNKSSAHKPEQISRNYHRLMLQLKTWETNHYTPDLNEEYF
                     RILDIETRNVQIFYNDFVNNVLIPLEQQPTTDLPEFITREDIFKLLGFKIFMPLAPIF
                     AEREKWMKRIAGTDGKNAHASESYNKFKKIFLNSHHKFVIEESLSHFEHGTLKYHHEW
                     LTAKRQIAKEVRSLRALQENWKEKKNKTDRKKASILFDFLDSFESIPNRLRSREEEGF
                     KRTEVYKKALSTDKLKAIELLNQHLFDYSNPQEELAERMKLIKALYENNILFEQHHTP
                     IRWLKNQLNIETVLDKKLAFLEQSLKAAENALNSYMEHAMPKTVTISTELRKESEKDS
                     VVLPEENKSPEAVSENQNTSHSNPVVQPQITPVQNKKNTGKVYLKVQEYEENLRKLGL
                     FTSQKSPFSEPLKKMPLSGENKLSL"
     gene            8252..9880
                     /locus_tag="ACF2SP_001791"
     CDS             8252..9880
                     /locus_tag="ACF2SP_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359575.1"
                     /translation="MFKTGEHNMILFYKLTLTKFTEDELKEDFAKIPSWATHLDLSNN
                     HLGERSSEELKRTFAKIPGKISLIKLRFNNLSQKSGKDLAKAFSKIPVSATTFDLGSN
                     QLGKTSGDALASAFAGIPTSVTRLILSQNELYKRTGEELATAFAKIPHSVTNLVLVSN
                     GLGKKKGKELATAFANIPSSVTTLDLALNDLAKIKSAELATAFTKIPTSVMQLDLSHN
                     NFGKKSGKKLAKIFANIPTSVTNLNLSCNELGKKSGKGLAAAFAKIPTSVTDLNIEDN
                     DLDKRTGKELGMAFAHISAFVNTLRLGDNNLGNKSGEELAEIFAKIPLSVTSLDLRYN
                     ELGKRNGKELATVFAKIPSSVTNLNLKFNHLGKRTGEELAMAFANIPTNIDSLVLANN
                     ELGAKSGEELAKIFSKIPASITSLNMGNDWFAVIEKLSQQKMVLSTITHAAIDYSSAQ
                     EHSAKLKLLGTVFPNLNKVTFMDIHGNNALVGQNPIEEAKWLKLCGIKNSVPSLLSQA
                     LFFVAKNKEHRSIRDYPSLLPKELTESVLSLLKC"
     gene            10144..12138
                     /locus_tag="ACF2SP_001792"
     CDS             10144..12138
                     /locus_tag="ACF2SP_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1959 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359576.1"
                     /translation="MLVSNTILKAALSHFEDRSDLIKQLDLSSDNDKNSFYKLVLAFW
                     LGSNITREKIIKHPLYIKCEAFIDLGYSCILFLDKQMSLLKQNTVAYKILQKTLFEIT
                     QHYNENYPLLTQNPQTLFSFFANILAKIDSKVCEDISHRKILELTQNTCLAELSRTQN
                     GPPDGLAATQVSNDVTSLMKVNPFNIGVAINKLITENFSKELFFPHYIKPTTDKHITH
                     KLLIPAWDGIVLEGLSVKGRKKTNNTVVLALIGHFQTEHHYLNTSFHEFQELFGTELV
                     LINHRNYSNRSNKFANSAEEIARDVIAFANHFRQKNKKIVLYGMCGGAAHMILAAHTL
                     SHQKIPFKLIVDRFSQKYINFVDFKTLSRARDFSRSNGQDCSRLLPGYKYYPGLMPYL
                     LILLFLLLFILVQLGLFLTKTNIDFAKLVRMIPEEDLLILQAKGEKIATLKKPFFTDI
                     IVHPENDMRAAVKDKRKQRKTILKNLCEHCLHAAGQAVFSAEMQKIFLQLFNCFDQCL
                     QLINNEKLMENTITNRPVDLHSKKLYTLTTRNKLPISQFIRGFFKQSPKMHAHLLDSI
                     KPYSSHLIVDALKQIYGNHPSMHSNLLQFSNHLALLLNDMKTNQFFISYMADRLSATQ
                     LADLNEPINALLRSELLQLIFKSSSEQNNQDIINNHRVSI"
     gene            12322..13095
                     /gene="lirA"
                     /locus_tag="ACF2SP_001793"
     CDS             12322..13095
                     /gene="lirA"
                     /locus_tag="ACF2SP_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LirA"
                     /protein_id="HFD2359577.1"
                     /translation="MFFSKDEKNPIKRALQGELLQDEPFIQLCTKIENYLMDTEAVNE
                     QLIELNEQLTMRLKEKGLKPGEKGATKQLRTLIQEILTEAGFREGMLQTIGNKPLKKE
                     DFMFLVSSGFMLKDSSLRASSHGELTHAIQWCLIILKQKKDSSFLENIPTSEICGRIY
                     KKLGHQDSLNPNYPFTCWDVLIDKLGEIDSRSPEWLSDHIQNDEDQIFPVLREVIKNR
                     TEKGKTEENKGKLQKKLENPPEHYEKHEEIENILMPKPK"
     gene            complement(13132..14643)
                     /locus_tag="ACF2SP_001794"
     CDS             complement(13132..14643)
                     /locus_tag="ACF2SP_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFD2359578.1"
                     /translation="MNDSEYESDSESEYELHTLPSKIHFVWVGGPIRAQYLRTIMDVA
                     AVALKSGFEVNLWVDNEMNYIKTSTQEGIDIPNLRIRNIHELEEGMRNDPFYEGDRYK
                     KFWEYANRERVGFRNLAAAADFFRFEILRQEGGYYFDTDIVFYLDENSQFVADEVAFG
                     IKAHVDLHTGSWKDPRGGGHTTHHVNTLGDVNNDIIAAIPDHEVMEDAILNAMQRHEE
                     YDSSPLPRESLKKRFGPYGNLMDAKRTPYQLEGPMDFRKYQTIAAGPGALQEALRDYM
                     EGVEVEDSSLLVTLKVGNESSRVANDKDKKPIMGIEVVSKCDKTWLTKTSKAKKRKAR
                     QKAFDTDSLAIEPALSKESGQAASKIDAYSDEESKYDDELTGEIGFQGNLRKEQFINE
                     LKEYIIIKKEEARQALEVKENEEGHDSEEKILSKHFKTRHIEITKGFSVEQKIAAVEK
                     LIELLQEKDGAELLNAIDYAALTSSRLGKLVQRYDDLILKEYVLPNEYGEKPY"
BASE COUNT         4872 a         2895 c         2535 g         4413 t
ORIGIN      
        1 agtttaattg ctaaaaataa tggctactcc tgcctggcgc tgcatgccgg ctcattactg
       61 tgtccacgca gcaaagataa tttaaacagc tattatgtga ttgaagcgaa tcagaaaaag
      121 tgccgcatta cctggcgtgt ttttgatcaa gaacacttta ctaatcgctc catctatttt
      181 attgatttta ataaaaaaaa tgcgtcattt gagcacataa acccagtcaa ggacgatact
      241 cgctaagaca atgcaatgca cttggtcaaa ataatcttca caggcaaaaa aaataaagaa
      301 taattgttgt gtctcgcatc tatagatgcc cccctttaag cgacaacagt tgcttatgcc
      361 aaaagaatca ccgaatgcac acgactacat tgtatttctc ttgtaagaac actggtattc
      421 caattgtcct acagcttcat accaaaagat tctgattccg atgttgagcc cccaatgaat
      481 tgatttaaga ctccagcaag catgctaaaa gagccctcca attgattcaa gccattcaat
      541 aagtcttgaa actgactaaa agaatcttga aattcattag cttcttttat catagattga
      601 ggcgtatttt ggcttaaatc tgtgagcaat tcgataagct cagcatgtcg cgcgttcagt
      661 tttttcaggt ttttattctt tctgcttgct ccctgcgata tttgcatgat tgttgtctga
      721 acgtctatta attcactgca atagctgaac attaatcttt cattgcttaa gttttccagt
      781 atggattcct tgagctcgct aaaactaatg tcgatattaa agtcaatttc taataattct
      841 ttcttcaatt cctgattggc tgaattaatt aaggattgaa aagcaaagtt ctttccggtt
      901 ttatgataaa atgccttcac cttacgaacc aactctttat attctttatc agcgattgct
      961 ttttctcttg atatttcctc atcactctca attgcttcag ccacagcgtc aggcaataat
     1021 tttttggtaa tatcgcttat ttcatcaacc aatgcctggt tttgaggact aaaaagtgag
     1081 gatgagtttt ttacattagt ctttaagtgc tcgtatcgag caatggactc ttccagggca
     1141 attttctggt caccctcaat atcatcagat aacaaacatg attttaatac attaattaag
     1201 tgagcataaa attgcttgtt attgagggtt aaacttttgt tttgctctaa cgcggcatat
     1261 aagggatgcc tggggatttc agacaaaaga agatgagcta aaggtaattt atgcaagcca
     1321 acaggttgca tcaaacttgc gccatttttt attaatacac taaagcagtc taccaacgat
     1381 tcactatgtt ctgagaagca gtactcaacg gcattggaat acgtttttac tttggtctta
     1441 attggataac tattgatagg caaacctact ttagtgacta aaaagcttaa aagatctgct
     1501 tttctctgag taagcgcaag caccattaca tcattattaa tcaaaggata ataaggcgac
     1561 aggctttgag cgtattcaaa ctctccagca aacaaagctc tttccaggag tgacttggct
     1621 tctttatttg cacggccttc gatttcattt agggcttgtg tttgctcttt tgtcagcaac
     1681 tcttccgttt ccaaattaat gtctttgatt ttccaatgca aatcaattaa aagaactgct
     1741 ttaatttttt tatcttccgt ctttaatagt tgagcaaaca atttttttat cttgctaata
     1801 tcgcgctcca ttttcacatc cacagggcgt ttcgctttga ctgatttgga aaataaggtc
     1861 gattgagcaa gcccgatatt tcctctgctg attttgggct ccttaggccc ttgctttgga
     1921 gacaaggcaa caacctctgc tttgtcatct tttttatgct cttcatcact ttttttaaca
     1981 ggctcaagaa ttggttccgg catagcggat acgtacttgt gtgttttccc caggtaaatt
     2041 gcttctctca gccaatgatt gctttcagaa atttggctta atgatttagt cgtcgtaatt
     2101 aattcatcaa gcttttccaa atagaccgct ctgtttttat ctaaatcctt acttagtgaa
     2161 gtcgtttcct cgtttaatct atggtaatca gcaagcaatt tagcttctat ttccttttgc
     2221 ttttttcgca acaattgcaa acaaggcagg cctagctgcc atacagaatg aatgaaatat
     2281 tccagatttt ctggtggaga aactttgaca tattgctcat catcatccaa taactcccag
     2341 gtagctgggc atgccagagc atcaaaacga ttcaaaaaca aatgcattcg ataggtttga
     2401 ccattttgat catggaagat cgcagtaaaa tgcgaaagtc ccaaatttgg attatgggga
     2461 tctgtcttct cataaagact aatatgcgcg tcatgcagtc tgaaaccttc agcagcaata
     2521 ttagccaagg gtaattctaa acgataatac ttcttatcct ttaaggtaat atcatggaca
     2581 ggtctaactt catctaataa tttaaaattt ggggattcag ctaaaatctg cgtcaataca
     2641 aagtaaaaca aatttggttt cataatctca aacagctcat tgaaatgagc caaggataca
     2701 taaggaaaca aatttgatca acgtgttttt tttaaaccca taagctattg tccacaacat
     2761 ggaatgacat cctcaataac aacaagcaaa tgccggtaag tgctgtatcg attcaataaa
     2821 aagccaacag tatgcagtta gaaagaatca ggacttacga aaaaccccaa gatcaaggca
     2881 aaaaatattt ttaatgaggg agtttagata aactaaatga ccgaattaaa aacttttttt
     2941 aacaaagaga ttgggatttt tcgtaagtcc tgagaatgta aaacctgtgt ttatcggatc
     3001 ctttcttaac tcattgaaca ccccaaagtg acagatttta tagcactttg agggaattga
     3061 gtaagtggct tttcgagaaa aaactcattt cattactata aattatgctt cggtaggaaa
     3121 atcttcaacc ggaggagcta tccataggct gcaatttgga gcaggcataa tataagttgt
     3181 tacaacgtga gaattcatac tattaagcat ggcttttcta gtcgtaagcc cagtattttt
     3241 aacagtgcaa accatatttt tttgtgccag gaaagccgcg caaccattat tttccggaga
     3301 attggtgtaa tcaatatctc gggttgtcat gagataaacc cgtgttttgt agggtaaaga
     3361 ttcagagagg agtttaaaac tgaaaggacc agctatatgg tgttgtcccc atgaagtaat
     3421 ttgctctgaa gccacggaaa tcacttgagg aagtccttgc acattcacag taattgtttc
     3481 agaactattg ggctcaacag gtaaattatt aatggtcagg gaaaaatctt cagccagtac
     3541 tttgggaaat tccacattaa atatagtagc ttgagcagct ggatcacctc ggtgatcagc
     3601 ctttacactg tcaaaaatat gccaaagttt attataagca ttggtacaaa gctgagcatc
     3661 ttttgaactg ggaagtacgc ttgcgaaaac tggattaata ccggcaacaa ttaagcctat
     3721 aaaaagaaat atatatttca tgtaactaac tccttgttat ctaagttgaa ttaaatttga
     3781 cttgagctga tacatcctat tgaaaaaaaa aagagtttta attttgaaaa aaactgtgag
     3841 cttaaagcaa actcaaaaac tcccatgata gataatagaa taaaacatat taaatacaaa
     3901 tttagcttct atatctgtat aagccctaag catagccctg tgtggtacaa actcattgat
     3961 ttaaaggatt aaagcgatca caaacatcag ggtatgaata aactataaac aattaaaaac
     4021 gcaacctgga cgccagtcac tacaaggcca ttttgtatca tggctttgac attctcactt
     4081 ccagcaaaac ccattgctgc gaacatatta gcgccagggt aagcataatt tgtgacgcga
     4141 gttgcaaaga gcatgcacaa ggcaaaagtg attagaggca attgcatttg gatgacagag
     4201 gggctaaaga gctggtgaag cattttgatg gttgcttctg cagcgcctgg cataccaaaa
     4261 gcgccggtaa agcctattaa gatcgataat actggtttcc ctcccaggtt aattaatggt
     4321 gttaacaatt cagttaatgc attaaatcct cctgcttgat gaattagcgc cataaaagga
     4381 tcaaataaaa taaacaaaaa gaagagatgc aagctttttt gcattccttc aaataacagg
     4441 ttaaaaattt gtttcaaacc gagtcgacag gataatccgg taattagcgc cagaaataac
     4501 atgacaaaga tcacataaga ggttttggct tgagtaaata aaccatagac aacacaaaac
     4561 aaaaaggaaa tcagaaaaat taatgtagcc cttttttgtt gtctattggg agtaaaaggc
     4621 tctgtgctgt tctcgttttc gcagggctca ttgatgtata gtttttgtaa tcgtgaagcc
     4681 ataaaccagg tggtaattaa agtcactaca gcaattggaa gagaagcgta aagaaatact
     4741 gtcccataac ttaatccagt gacgccaagc aaggttacaa ccgggggagc aaaaggtcct
     4801 agaattaaag cctcttcagc tgaggcctgc atgagtacag ccaaactggg gcgtgacaag
     4861 cctactgacc ctgcaatagg acgcaaactg ggggccagaa tggctaaacc tcccgccaat
     4921 gttcccagca aaccaacaat aataaaggat gagaggacta taccaatttt agcgcgtcgc
     4981 tgagtattaa ccccaactcc ataaacaatt tggtgcacta aagtatgact tacctgggta
     5041 tgcgttaaaa cttcgcccaa ccctcgacct aacatgatga taaaaccaac caaagccata
     5101 aatgaaccca aggcgtcagc catagcattc cctatgtcaa tgggagtgct tatattccac
     5161 aaaaaaccta ggccaacgca aactgctgtc gctactaatg gatcaacgtt atgaaataac
     5221 agtaaaacat aaagaataat aatagataaa ctggctagct gaatgtagaa agacattagc
     5281 ggatcacccc aattagattc agcccttgtg tgatgccttc aatccctaat ccaggtttgt
     5341 ctgccaggat aattttattg ctgagatttt gtgccccacc aagaatataa ttctcgcgga
     5401 tcaaaaaaat aggatcgaga tctgcataag aaatatcggg cttacttaat gcaaaactgg
     5461 ctattgcagc aactccaatc ggcgactcca gcatacatcc aaccattatt tgcattttgg
     5521 ccgcttgagc aatatggtag atagattgag ctttctcaat gcctcctgat ttcatcaact
     5581 ttatattgac cccatcacaa gcattaatct ttgcgatatt caatgcatct tctggtgaaa
     5641 aacacgcttc gtcagcaata attgcacaac caacctgatc actaatcgtc tttaagtcag
     5701 gcagatttcg agcattaacc ggttgctcca ccacaggaac attcaaattc tcttttttga
     5761 acgcagtaat ggcgattaat gcctcttcat aagaccaccc ctgatttgca tctgctagca
     5821 gagtaatgtc attgcctact gcctgacgaa tagctcgaat tcgattgatg tcgtccactg
     5881 gatttaatcc caatttgatt ttcagggttt tgtgccctcg atgaattaaa tcaatcgcat
     5941 cactcaccat ctcctcaact tctttaacgc ttattgtaat acacgagttg atacaattgg
     6001 tatttcctcc caattgctta taaagaggca agccacaata ttgggcaaat aaatcatgca
     6061 aagcgatatc aatcgcggcc ttggctgagg agttaccttc tattgcccga tcattcattt
     6121 gcagtaataa attgaactca gaaatatcgc gtccgataag aggaggcccc aaggcacttt
     6181 taatagcagc aataatggat tccttgctgt ctccagttat agcaggtgtt gatgcagctg
     6241 acccataacc aatctttcca caatccgttt ttattaatac cacaacatca tcaacgtatt
     6301 ctgtgcgcct caccgctgta ataaacggac gacgtaatgg aattgttagg tttgcgatat
     6361 ggatttcttt aatattcaag taagcaccag tttatttatt gtttgcacgg taaccctaat
     6421 gagatattaa aatattaaga gttaatttgg caaaacatgt ttttaaatta tatctctcac
     6481 caaataatat taacttgata ccctgcttca atacatcact tttctattct tgaatcaact
     6541 caataaaatg aaccaactat ttctgacgaa cactaaaaac agatacggat ttcatgtatt
     6601 caaaaacaac tgtcttttgc tatcatccaa atttcttatc atccccaaaa tcaatccatt
     6661 tgtattaacc tggagatacc catgcttttt aaactcaata aatccagcgc ccacaaacct
     6721 gaacaaatta gcagaaatta ccaccgtttg atgctgcagt tgaaaacatg ggaaacaaat
     6781 cactacacac cagacttaaa tgaagaatat tttaggattt tggatattga aacaaggaat
     6841 gttcaaatat tctataatga ttttgtgaac aatgtattaa tccctcttga acagcaacct
     6901 acaactgatt tgccggaatt catcacccgg gaagatattt tcaaactttt aggctttaaa
     6961 atatttatgc ctttagctcc gatatttgcg gagagagaga aatggatgaa gcggattgcc
     7021 ggtactgatg gaaaaaatgc tcatgccagt gaaagctaca ataaattcaa aaaaatcttt
     7081 ttaaactctc atcataagtt cgttattgag gaaagcttat ctcattttga acacggcaca
     7141 ttaaaatatc accatgagtg gctaactgcg aagcggcaaa ttgccaagga agtcaggtca
     7201 ttacgtgcat tacaagaaaa ttggaaagag aaaaaaaaca aaactgacag aaaaaaagct
     7261 tctatcctgt ttgattttct tgatagtttt gaaagtattc ccaaccgatt acgctcaagg
     7321 gaagaggagg gattcaaacg aacagaggtc tacaaaaaag cactttccac agacaagctc
     7381 aaagcaatag aactcctgaa tcagcatctt tttgattaca gcaatcctca agaggaattg
     7441 gcagaaagaa tgaagcttat caaagctctg tatgaaaata atattctctt tgagcagcac
     7501 catactccaa taagatggct gaaaaaccaa ttgaacattg aaacggtact tgataaaaag
     7561 ctggcgtttc tggagcaatc gttaaaagca gcggaaaatg cgctaaattc ttatatggaa
     7621 catgcaatgc ctaaaacggt cacgatttcc actgaactaa gaaaagagtc ggaaaaagat
     7681 agcgtcgtgc tccctgagga aaataaatca cctgaagcag tttcagaaaa tcagaataca
     7741 agtcattcta atcctgtagt tcaacctcag attactcctg ttcaaaataa aaaaaataca
     7801 ggaaaagttt atcttaaggt gcaagaatac gaagaaaacc tccgtaagct tggtttattt
     7861 acttctcaaa aatccccctt ctctgagcca ttaaaaaaaa tgcctctttc tggtgaaaat
     7921 aaactctcgc tataaataac aaatggagca gatttgcagt ctttctatgt aaattcattc
     7981 caaatttctc cctgaatttg aatttatttt ttctaacaag ctacttcaat atacctatag
     8041 agccattaat gaacattatg atatattatt ggctcagcga ttgtcttctc cgaatggcat
     8101 aacaggagaa ctggttttcc tagttttaat gattaaacgg gttttcgcta agatgaggtt
     8161 tgcaaaagca aacttatgct tatcttagtg atatccattt gattcgcctt catggctctc
     8221 ctcatctttt gaatttctta ttcattaaag gctgtttaaa acaggagagc ataatatgat
     8281 actattttat aaacttactc tgacaaaatt tacagaggat gagttaaaag aagattttgc
     8341 caaaattccc agctgggcaa cccaccttga tttatcaaac aatcatcttg gcgaaagaag
     8401 cagtgaagaa ttaaaaagaa cctttgccaa gattccaggc aaaataagcc ttattaaatt
     8461 gagatttaac aacctcagcc aaaagagtgg caaagacttg gcaaaagcct ttagcaaaat
     8521 accagtttcg gcgactacct ttgatttagg ttccaatcaa cttggcaaaa caagtggaga
     8581 tgcattggca agtgcattcg caggaattcc aacatcagta actaggctta tattgagcca
     8641 aaatgaactt tacaagagaa ctggggagga gctggcaact gcctttgcca aaatcccgca
     8701 ctcagtaacc aatcttgttt tagtgagcaa tggattaggc aaaaaaaagg gtaaggaatt
     8761 ggcaacagct tttgccaaca ttccaagctc agtgactacc cttgatttag ctcttaatga
     8821 tctcgccaaa ataaaaagcg cggaattggc aacagccttt acaaaaattc caacttcggt
     8881 aatgcaactt gatctgagcc ataataactt cgggaaaaaa agtggaaaga aattggcaaa
     8941 aatttttgcc aacattccta cctccgtaac taaccttaat ttaagttgca atgaattggg
     9001 caaaaaaagt ggcaagggat tagcagctgc ctttgccaaa attccaacct cagtgacaga
     9061 ccttaatata gaagacaatg acctggataa gagaactggc aaggaattgg gaatggcttt
     9121 tgcacacatt tcagccttcg tgaatactct caggttagga gataataacc taggcaacaa
     9181 aagtggagaa gaattagcag agatctttgc caaaattcca ctatcggtga cctcacttga
     9241 tttacgctac aatgagcttg gaaaaagaaa tggtaaggag ttagcaactg ttttcgccaa
     9301 gattcccagc tcagtgacta atcttaattt aaaatttaat caccttggta aaagaactgg
     9361 agaagagctg gctatggcct ttgccaacat cccaaccaat atcgattcac tcgttttagc
     9421 gaataacgag cttggggcta aaagtggaga agaattagct aagattttta gtaaaattcc
     9481 agcttctata accagcttga acatgggcaa cgattggttt gctgtcatag aaaaattaag
     9541 tcagcaaaaa atggttctat ctacaataac ccatgccgcc attgactata gctctgctca
     9601 agagcactca gcaaaactca aattgcttgg aactgttttt cctaatctaa ataaagtcac
     9661 ttttatggat attcacggaa ataacgccct agttggtcaa aatcctatag aggaagccaa
     9721 atggctgaaa ttatgtggca ttaaaaattc cgtgccctcc ctactcagcc aagctttgtt
     9781 ttttgttgca aaaaataaag aacataggtc tattcgagat tacccaagct tgcttcctaa
     9841 agagcttact gaatcagttt tatcgctatt gaaatgctaa agcaagattt gtatttctca
     9901 caaggatcag ctcaatgatt atccatttca catggtgcca ctttaatgat ggcgccatct
     9961 ttataatgta gcagaggtgt gatttaacct catccacaga ttgtataggt agtggtattg
    10021 cagcaaatat caggttacct gaattactca ccatagaaaa actcatccaa atatgaaata
    10081 tttttgcaaa ttttgaatat aaaattgcta taatacagcg attttttact attttttgga
    10141 ctcatgttag tttccaatac aattctcaag gcggcattaa gccatttcga agacagaagt
    10201 gatttaatca aacaactgga tttgtcttct gataacgata aaaactcgtt ttacaaatta
    10261 gtgttggcgt tttggttagg ctcaaacata actcgtgaga aaattataaa acacccttta
    10321 tacataaaat gtgaagcttt tattgatttg ggttactcgt gcattttgtt tttggataag
    10381 caaatgagct tgttaaagca aaatacagtt gcttataaga tcctgcaaaa aaccttattc
    10441 gaaataactc aacattataa tgaaaactat ccgttattaa cccagaatcc ccaaacgcta
    10501 ttttctttct ttgccaatat tcttgcaaaa attgacagca aggtatgtga agatatttct
    10561 caccgaaaaa tccttgaact gactcagaat acgtgcctag ctgaattatc gcgcactcaa
    10621 aatggcccgc cagatggatt ggcagcaaca caagtgtcta atgatgtcac cagtttaatg
    10681 aaagttaatc cgttcaatat aggtgtcgct atcaataagc taattacgga aaattttagc
    10741 aaggaacttt ttttcccaca ctacataaag ccaacaaccg acaagcatat tactcataaa
    10801 ttattaatac cagcctggga tggtattgta cttgaggggc tttccgttaa gggaagaaaa
    10861 aaaacaaaca ataccgttgt tttagcatta attggacatt tccaaacaga acatcattat
    10921 cttaatacca gctttcatga gttccaggag ctttttggca cagaattagt gcttataaat
    10981 catagaaatt attccaatcg atccaataaa tttgccaaca gtgctgaaga gatagccagg
    11041 gatgtgatcg ctttcgcaaa ccatttcaga caaaaaaaca aaaaaattgt actgtatgga
    11101 atgtgtggcg gggctgcgca tatgatttta gcggcccata cgctatccca tcagaaaatc
    11161 ccatttaagt taatcgttga cagattctct caaaaataca tcaatttcgt agactttaaa
    11221 acactctcga gagccagaga tttttcacgc tcaaacggac aagattgctc gagattgctt
    11281 ccaggttaca aatattatcc cggtttaatg ccatatttgc tcattctcct ttttttattg
    11341 ttatttattt tagttcaatt gggtctattt ttaacaaaaa caaatattga tttcgcaaag
    11401 cttgtgcgga tgattcctga agaagacctt ttgattttac aagccaaggg agaaaaaata
    11461 gcaaccttaa aaaaaccatt ctttacagac atcattgtac acccagaaaa tgacatgcgg
    11521 gcagccgtaa aagataaaag aaaacagaga aaaaccatct tgaaaaattt atgtgagcat
    11581 tgtttgcatg ctgctggtca agctgttttt tcagccgaga tgcaaaagat ttttcttcaa
    11641 ttatttaact gctttgatca atgcctccag ctaataaaca acgagaaatt gatggaaaat
    11701 accatcacta atcgccctgt agatttacac agtaaaaaac tttacaccct tacaaccaga
    11761 aataagttac ctatcagtca gttcattaga ggttttttta aacaatcacc aaaaatgcat
    11821 gctcatttgc ttgattcaat caaaccatac tctagtcacc ttatagttga tgctttaaaa
    11881 caaatttatg gaaaccaccc atcaatgcat tctaatttat tgcaattttc taatcatttg
    11941 gctttgcttt taaatgatat gaaaactaat cagtttttta tttcttatat ggctgatcgt
    12001 ttatctgcta ctcaattagc tgatttaaat gaacctataa atgcgctgtt aagaagcgaa
    12061 ctacttcaac taattttcaa gagttcgtct gaacaaaata atcaagatat cataaacaat
    12121 catagggtat ccatttaatg cattcattga ccccactaat tgggctatcg ttgatactga
    12181 atgtcaagat taatgtaaat gcgcttaaac aatgtgctca tcaacactca aataaaccaa
    12241 taaataagca ataacaaata tttataaaca atatttatta aaaataatct atacttaaaa
    12301 aattagcgtg ttggaggtaa tatgtttttc tctaaagatg aaaaaaatcc aataaaaaga
    12361 gctcttcaag gtgaattatt acaagatgag ccctttatac aactatgtac aaaaatagaa
    12421 aactacttga tggataccga agctgtaaac gagcaactta ttgaattaaa cgaacaactg
    12481 acaatgaggt taaaggaaaa aggactcaaa cccggagaaa aaggagcaac aaagcagtta
    12541 agaacactaa tacaggaaat actaaccgag gctggcttta gagagggaat gttacaaaca
    12601 atagggaata agccattaaa aaaagaagac ttcatgtttt tagtatcatc aggatttatg
    12661 ttaaaagaca gcagtttaag agcatcatcc catggtgagc tgacccatgc aattcaatgg
    12721 tgtttaatca tattgaaaca aaagaaagac agcagttttc ttgaaaacat acctactagt
    12781 gagatttgtg gccgaattta taaaaaacta gggcaccaag attctttgaa tcctaattat
    12841 ccttttactt gttgggatgt acttatcgat aaattaggag aaatagactc cagatccccg
    12901 gaatggcttt ccgatcatat tcaaaacgac gaggatcaaa tttttcctgt gttaagagag
    12961 gtaataaaaa acagaactga aaaaggcaaa acagaagaaa acaagggaaa attacaaaaa
    13021 aaactggaaa atccgccaga acattatgaa aaacacgagg aaattgaaaa tattctcatg
    13081 ccaaagccaa aataagtttg aactactaca gtccaactaa tccatttgaa ttcagtatgg
    13141 tttttcccca tactcattag gcaaaacata ttctttaagt atcaaatcat cgtatctttg
    13201 gactaactta cctaggcgag aactggtaag cgctgcataa tcaattgcat taagcagttc
    13261 cgccccatcc ttttcctgga gtaactcaat aagcttctct acggcagcaa ttttctgttc
    13321 aacagagaac ccttttgtta tctctatgtg acgtgtttta aaatgcttac ttagaatttt
    13381 ttcctcagaa tcatggcctt cttcattctc tttcacttcc aaagcctgcc ttgcttcttc
    13441 tttttttatg ataatgtact ctttcaattc attgataaat tgttccttgc gtaagttgcc
    13501 ctggaatccg atttctcctg ttaattcatc atcatactta ctttcttcat cagaataggc
    13561 gtctatctta cttgctgcct gcccagattc ctttgaaagt gctggctcta tagctagcga
    13621 atcggtatca aaggcttttt gccttgcttt tcttttttta gctttacttg ttttagtgag
    13681 ccatgtttta tcacacttac tgaccacttc gattcccatg atcggttttt tatccttatc
    13741 atttgctact cttgagcttt cattaccaac ctttaaggta actagcaagc tggaatcttc
    13801 tacttcaaca ccttccatgt aatcacgcaa tgcctcctgt aatgcaccag gaccagctgc
    13861 aatagtttga tattttctaa aatccatagg tccctccaat tgataaggag tgcgtttagc
    13921 atccatcaga ttgccatatg gaccgaatct ttttttaaga gattcccggg gtaaagggga
    13981 tgagtcatac tcttcatgac gctgcatagc atttaggatt gcatcttcca tgacctcatg
    14041 atcagggatc gcagcaataa tatcgttatt gacatcccct aaagtattga catgatgggt
    14101 agtatgtcct cctccacgtg gatccttcca tgatcctgta tgcaaatcta cgtgggcttt
    14161 tataccaaaa gcaacctcat ccgctacaaa ttgtgagttt tcatccaaat aaaaaacgat
    14221 atcggtatca aagtaatacc caccctcttg ccttaaaatt tcaaaacgaa aaaaatctgc
    14281 agctgcagca agatttctga aaccgactct ttctctattg gcatattccc aaaatttttt
    14341 gtacctgtcc ccttcgtaaa aaggatcatt tctcatgcct tcttctaatt catgaatatt
    14401 ccttattctt aaattaggta tgtctattcc ttcttgagtt gatgttttaa tgtaattcat
    14461 ttcgttatca acccataaat taacctcaaa accacttttt agtgctactg cagcaacatc
    14521 cataatagta cgtaaatatt gagctcgaat aggacctcca acccaaacaa aatgtatttt
    14581 tgatggtagc gtatggagct catattcaga ttcagaatca ctttcatatt cagaatcgtt
    14641 catgaaactc tcgcataata attcaacatt ctttaattat agtacacccc tatctaatta
    14701 agctgaaatt ctgag
//
LOCUS       DAXLDT010000073        14540 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXLDT010000073 DAXLDT010000000
VERSION     DAXLDT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14540
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.073"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1293
                     /locus_tag="ACF2SP_001795"
     CDS             <1..1293
                     /locus_tag="ACF2SP_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359579.1"
                     /translation="NYQNLKKEFLQFLHKADSEDPFLKKIHGLLEQDNPSILYLIEQE
                     LESSYYKDKYSQKFNQLILLMKLKVAHTPNPVVTPNLLQDISLLQLEGEIEKQRMIAI
                     IDGQEPLARALKYLSIGCTTTAARTKELQKIIVQHGTNLQAYRHQDKCRELGGRIVLH
                     CSQAMLPGMGLKSGMCHGLARHWAEDVIKKDRFMGFRGNQEAFLQPNKVTDKILQAIP
                     NFNDFVRLNPKIYETQQSQQEHVSLNYEDTVGSEPDKQFKEFTDRIMSELDKYGKSAT
                     FVSYANSHSGHVVAIHKRTKPTPQGFLIDYFDANAGWMQFKDDKSFQEFLTYYLNDRH
                     AKEKLKSIAFETLCYPSSYTHMLDKLCEKTPQPTPQRQRAQSLSAEIERNTPQMKPQI
                     SEKTEKTEKAEKAEKAEKTEKTEKTENEQSRDARRFPI"
     gene            complement(1334..1654)
                     /locus_tag="ACF2SP_001796"
     CDS             complement(1334..1654)
                     /locus_tag="ACF2SP_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359580.1"
                     /translation="MKRMIGFMVSVMLTFAVNAADSQVNTHKHQTDKGKVTPGMSIQR
                     NIEQSKAITADAASTNTTEKVTSQQMKEHKNSVKEANYNAQKKEEEAKTATDTQKHEA
                     DSPS"
     gene            complement(1704..2303)
                     /gene="ruvA"
                     /locus_tag="ACF2SP_001797"
     CDS             complement(1704..2303)
                     /gene="ruvA"
                     /locus_tag="ACF2SP_001797"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFD2359581.1"
                     /translation="MIGWLHGQIIDKHQPGKLVLDVNGVGYDVETSLNTFFQIENGNQ
                     PIGLHIHTIVREDALLLYGFLDKEERSLFRSLIKVNGVGPKLAMTVLSSISPKEFIQC
                     IHQENAALLTKLPGIGKKTAERLVVEMRDSIKQFDGSVSDTFQKQAGSTHSQQEAISA
                     LEALGYKPQEAWKVVNKIDNGNKSCEQLIREALQILSSR"
     gene            complement(2300..2824)
                     /gene="ruvC"
                     /locus_tag="ACF2SP_001798"
     CDS             complement(2300..2824)
                     /gene="ruvC"
                     /locus_tag="ACF2SP_001798"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFD2359582.1"
                     /translation="MTIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDVELSH
                     KLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRAKICE
                     YSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
                     LIGSRGTKRRRMRL"
     gene            complement(2824..3567)
                     /locus_tag="ACF2SP_001799"
     CDS             complement(2824..3567)
                     /locus_tag="ACF2SP_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD2359583.1"
                     /translation="MAGHSKWANIKFRKGVQDAKRGKIFTKLIREITVAARMGGGDES
                     SNPRLRDAVKKALNANMKRDTIDNAVKRGVGGADGEAMIAMRYEGYGPGGVAILVDCL
                     SDNKNRTVSEVRHAFSKHGGNLGTDGSVSYLFTNQGEILMASNQPEDKVMEIAIDAGA
                     SDVAVEDSQIEIITPVEAYHTVLNALQDAGLEVEQSHLTMRAQTLVPISDETAESLIK
                     LIDMLEDLDDVQEVYSNAEFSEKILESMN"
     gene            3714..4964
                     /gene="hmgA"
                     /locus_tag="ACF2SP_001800"
     CDS             3714..4964
                     /gene="hmgA"
                     /locus_tag="ACF2SP_001800"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HFD2359584.1"
                     /translation="MYLQGFGNYHHSEAVKGALPTNQNSPQHCSLGLYAEQLSGTSFT
                     RPRHNNLRSWLYRILPTVTQGTYYPYEFNVMQPFVDELSPNAMRWSPLYNSSQIKCDF
                     VEGLFHIAGSPLVNTYTYYCNHSMSDKYFANNDGELLFVPYAGEIHLHTEFGKLILSS
                     GSIAVIPRGVKFKVEVISKEAKGYLCENSGNPLTLPQLGPIGANGLANPRHFQYPVAA
                     FENSGGEHTIICKNQKKLWFTVCNHSPLNVVAWHGNYAPYCYDLSLFNTINTVSFDHP
                     DPSIFTVLTSESEIPGVSNLDFVIFPPRWMVAEHTFRPPYFHRNYMNELMGLVYGEYD
                     AKKEGFIPGGISIHNCMTPHGPDYESYEIAASQDLKPNYINSLAFMFETKDYWQVTEQ
                     AYRHPSRQMDYLNCWQGFKIEFSQ"
     gene            complement(5067..6092)
                     /gene="rpoS"
                     /locus_tag="ACF2SP_001801"
     CDS             complement(5067..6092)
                     /gene="rpoS"
                     /locus_tag="ACF2SP_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFD2359585.1"
                     /translation="MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDIEPDIEKMEEELD
                     ELPEFTDDEAFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
                     ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
                     WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKP
                     LEDVQKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLL
                     DKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVG
                     LTQEDLF"
     gene            complement(6178..6921)
                     /locus_tag="ACF2SP_001802"
     CDS             complement(6178..6921)
                     /locus_tag="ACF2SP_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFD2359586.1"
                     /translation="MMRLFRKQLICIFFILSVAGCGTRSDLAPVTELKWQPYSKYQKF
                     HTVRSGETLYAIAFRYDTDYRRLAIINHLRPPYSLRVGQVLSLKGIVPKSNHFHSAPR
                     ANLHITHKPKQKIIYSPAYNVRSSSGWLWPVTGRVVTTFIPSQGKKGINIACNRGDKV
                     RASASGVVAYAGSGLPGYGNLIIIKHSNEYLTAYGNNARNLVTEGQRVSAGQVIAEAG
                     LIDRSYWGVHFEIRRAGVPVNPLNYLQKG"
     gene            complement(6940..7698)
                     /gene="surE"
                     /locus_tag="ACF2SP_001803"
     CDS             complement(6940..7698)
                     /gene="surE"
                     /locus_tag="ACF2SP_001803"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFD2359587.1"
                     /translation="MKILVSNDDGVLAPGIKILANELSTLGEVKVVAPDRNRSGASNS
                     LTLTQPLRVKQLDNGYYSVDGTPTDCVHLALTGFLEPIADIVVSGINEGANLGDDVLY
                     SGTVAAAMEGRYLGLPAIAISMVGDNIQYYETAAIIAKQLVIKLSANKLPSQTILNVN
                     VPDLPLSQIRGLQVTRLGTRHSAEPIIKEYDPRGRPIYWVGPPGIEADAGAGTDFFAI
                     KTGHVSITPLHLDMTHYKLFDHLSNLLNEICIEN"
     gene            complement(7701..8084)
                     /locus_tag="ACF2SP_001804"
     CDS             complement(7701..8084)
                     /locus_tag="ACF2SP_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359588.1"
                     /translation="MNKYLVIILITLGLVSCQFKKDESYYRSHPSELQNALKLCPGTQ
                     PDGLSCQQLEAIGRRMNGLAYQLQYNPQEFGNKILALQQVIANQQREIQSKKDNAELQ
                     DSLKRNQEDLAYYLAVVKWLESPES"
     gene            8164..9879
                     /locus_tag="ACF2SP_001805"
     CDS             8164..9879
                     /locus_tag="ACF2SP_001805"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HFD2359589.1"
                     /translation="MLDFSLNRDPQTGELYIETSLCGKPLLTTPQLNKGTAFTQEERK
                     DFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRLQQHIYLNNLHDKNQIVFYKLLSRHL
                     GEMLPIIYTPIVGAAAKRFSHEYRQPRGLYIAHSDKNQLEEIINNRSNPDIDLVVVTD
                     GEGVLGIGDQGIGGMDIPVAKLMVYSLCGGIDPTRTLPIFLDVGTNNQELLNDPMYLG
                     CRHPRIKSSEYDDFIKTFVNEIHKQFPNAFLHWEDFGRGNARRILDQFQDELCTFNDD
                     IQGTGAVTLAAILAACDVTGLPLHEHRIVVFGAGSAGTGISDQIVDAMVKSGLSLTDA
                     YDRFWLIDKQGLLLATDQELTDAQKPYAWNPIDIQSWEINNKQHPSFTDTIRHVKPTI
                     LIGCSAQTGAFSQDIVETMSTACERPIIFPLSNPDEKCEAQPSDILAWSKGRALIATG
                     TAFAPIEYQNRMVQIAQCNNALVFPGIGLGVLAVSASRLTKDMILAAAQTLSKFAPSK
                     KDSFLPLLPSLDNAQIVAKEIAIAVAQCAIDSGYAQKNQDKDLPRLIDELFWEPRYLP
                     FRKRG"
     gene            complement(9936..10307)
                     /locus_tag="ACF2SP_001806"
     CDS             complement(9936..10307)
                     /locus_tag="ACF2SP_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HFD2359590.1"
                     /translation="MKKLFSVLLMGFSALAYANNEVVINVSSKNPSFVVSLAANPTTG
                     YQWSVVEFDKNLLTLSNSIYEKPKTNLIGAGGKMLFTFNLNKGKTYPTQTKMVFKYAR
                     SWEPESGTIKNVTINFANPSD"
     gene            complement(10343..10660)
                     /locus_tag="ACF2SP_001807"
     CDS             complement(10343..10660)
                     /locus_tag="ACF2SP_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HFD2359591.1"
                     /translation="MIIRNYLLAIVFLMIVYPNAYAMRCGDKLVYEGDSKYTVLSKCG
                     EPLDKLVYEENVPLYNAAGYQIGYGTNTVETWIYQKSPVDFQYELIFDYGKLKQINVN
                     RNP"
     gene            complement(10743..12554)
                     /locus_tag="ACF2SP_001808"
     CDS             complement(10743..12554)
                     /locus_tag="ACF2SP_001808"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2359592.1"
                     /translation="MNIYNKKSEELPKTLGRFFWHFIKKQPLAFTVFFVAPIAMVLEN
                     NAIPYSLKMIIDALGGHSGEENIFSAVAPALWLGGGAWFALIVILRLQNWWQGYVIPR
                     FQADVRMSVMEYLSRQSYHYFSNQMAGGLANKVNDLPRALDSIFNIITWYAIAAFASI
                     IVALILMCTINYWFAVILLSWIIFQLYISYRLSKKVDQYAKENAEDKSQLSGKIVDSL
                     SNSSVVKLFARSRDELAYISASQNMEVQSNKKLSIYMNIFRLYLDIPVTVMLVAMVYF
                     LLTFWNKKLITTGDLVFIFNVSFSIMTQIWYLCHAIAEFFREIGIARQAIALLSAPIE
                     VEDAPDAKPLIVTEGKIEFDSVTFHYNQGKKVFENKSVIIKPKQRVGLVGFSGSGKTT
                     FIHLILRLFNVESGKILIDGQDISQVSQDSLRSAISLIPQDTTLFHRTLMENIRYGNP
                     DASDDEVVMASKQACCDDFISLLPDGYNTLVGERGIKLSGGQRQRVAIARAILKNTKI
                     LILDEATSQLDSLTEEAIQNSLWQLMESKTTIVIAHRLSTLLHMDRILVFEAGKIIED
                     GSHEELLAKDGLYKSMWDAQVGGFLPESDRDRDNWDE"
     gene            12790..14421
                     /locus_tag="ACF2SP_001809"
     CDS             12790..14421
                     /locus_tag="ACF2SP_001809"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFD2359593.1"
                     /translation="MEHETMEFDVIIVGAGPSGLSAAIKLKQLALASQKEITICILEK
                     GAQVGAHILSGAVLEPRSLKELLPENWQQAPLDTPVNKDLFYFLTQNKSFKLPTPKPM
                     HNEGNFIISLGELCKFLAEQAEALGCEIYPGFAAAEILYNDKNQVIGVATGDVGIDRQ
                     GKKTDNYQPGMHLLAKQTLFAEGCRGQLSQTLMNRYHLRDHSQPQTYGIGIKEIWQVD
                     ASRHEPGKVIHTVGWPLDHATYGGSFLYHLSEQRVAIGFVVGLDYKNPWLNPFGEFQR
                     FKTHPLIKSVLTGGERIGYGARALNEGGWQSLPKLTFPGGALIGDAAGFLNVPKIKGI
                     HTAMQSGMLAAQACFDALSEEKSGQFELSAYSEKINQSWLKKELYAVRNIRPGFKYGL
                     FPGLINAALETYVTRGYSPWTLKNHADHKSLIPAEKAKKINYPKPDGVLTFDKLSSVF
                     LSNTFHEENQPCHLKLKQPKLAIEVNLNQYASPESRYCPAAVYEIIEDVEKGPRLQIN
                     AQNCIHCKTCDIKDPRQNIVWQAPEGGGGPNYSGM"
BASE COUNT         4513 a         3043 c         2570 g         4414 t
ORIGIN      
        1 aattatcaaa atctaaagaa agagttttta caatttttac acaaggctga tagcgaagac
       61 ccctttctta aaaaaattca cggtctttta gaacaagata atccatcaat cctgtatcta
      121 attgagcaag aattggaatc ctcatattat aaagataaat attctcaaaa atttaaccaa
      181 ttgattttgt tgatgaaatt aaaagtagct catactccaa atccagtggt tactccaaac
      241 ttgttacaag acatatcctt gcttcaattg gaaggagaaa ttgaaaagca acgaatgata
      301 gccatcattg atggacagga gcccttagct cgtgcgttaa aatatttatc gattggatgc
      361 actacaactg ctgcgagaac gaaggagtta cagaaaatca tagttcagca tggtacaaat
      421 ttgcaggcct atcggcatca agataaatgt cgtgaactag gtggtcggat tgttctccat
      481 tgtagtcagg caatgctacc aggaatgggt ttaaagagcg gcatgtgtca cggcttagct
      541 aggcattggg cagaggatgt aataaagaaa gacagattta tgggctttcg agggaatcaa
      601 gaagcatttc ttcagccaaa caaagtaaca gataaaatat tacaagcaat accaaatttt
      661 aatgattttg tccgtcttaa tcctaaaatc tatgaaactc aacaatctca acaagaacat
      721 gtctctctga attatgagga tacagtggga tctgaaccag ataaacagtt taaagaattc
      781 actgatcgaa taatgtctga attagacaag tatggtaaat ctgctacctt tgttagctat
      841 gctaatagcc attccggaca tgttgttgct atccacaaac gaacaaaacc aacacctcaa
      901 ggtttcctaa tcgactattt tgatgctaat gcaggttgga tgcagtttaa agacgataag
      961 agttttcaag agtttttaac ctattactta aatgatagac atgcaaaaga aaaacttaag
     1021 agcattgctt ttgaaaccct gtgttatcct agctcctaca ctcatatgtt ggataagctc
     1081 tgtgaaaaaa caccacaacc tactcctcag agacaaagag cacagagctt gtcagcagaa
     1141 atagaaagaa atactcctca aatgaagcca caaattagcg aaaaaactga aaaaactgaa
     1201 aaagctgaaa aagctgaaaa agctgagaaa actgagaaaa ctgagaaaac tgagaatgag
     1261 caaagcagag atgcaagaag atttcctatt taaagaaatt ttctaaggtg ataatatgaa
     1321 aatagtgcca cctttagctt ggtgaatctg cttcatgttt ctgggtgtca gtcgcagtct
     1381 ttgcttcttc ttccttcttt tgagcattat agttcgcttc ctttacactg ttcttatgct
     1441 ctttcatctg ttgggaagta accttttctg tggtatttgt agaggctgca tcagctgtaa
     1501 tagcctttga ttgttctatg tttctctgaa ttgacatacc tggggtcact ttacccttat
     1561 cagtttggtg tttatgagta ttgacttgag aatcagcagc attgacagca aaggttagca
     1621 tcacacttac cataaaaccg atcattcttt tcataaatgc tcctgtttat tatcgagaat
     1681 tttatcagat cacagttaca atctcatcgc gaagaaagaa tttgcaaagc ctcacgaatc
     1741 aattgctcac aactcttatt gccattatca attttattca caactttcca agcctcttgt
     1801 ggcttatagc ctaatgcctc caacgcgctg attgcctctt gttgactatg agtgctacct
     1861 gcctgctttt ggaaagtatc ggaaaccgaa ccatcaaact gctttatact gtctctcatt
     1921 tcaactacta agcgctctgc agttttcttg cctatgccag gtaatttggt taataaagca
     1981 gcattctcct ggtgaataca ctgaataaat tctttaggag aaatactgga taaaacagtc
     2041 atagccagtt tggggcctac cccattcact ttaattaatg atctgaataa agaacgctcc
     2101 tctttatcca ggaagccata caataacaaa gcatcttctc gaactatggt gtgaatatgt
     2161 aatccgatag gttggttacc attttctatc tgaaaaaaag tattcaaaga tgtttctaca
     2221 tcatatccta cgccattgac atccagaacc aattttcctg gctgatgctt atctataatt
     2281 tgaccatgga gccagcctat catagtctca tcctcctgcg tttcgttcct cttgaaccga
     2341 ttaattttga caaaccattt ctcatatggc tatgacatat tgctattgcc aaagcatcgg
     2401 cagcgtcttt ttgaggagct cgattgagct gaagaagctc tacaaccata tgacttactt
     2461 gatctttttc ggcagcccca taaccaacca cactttgttt tatttctctt gctgaatact
     2521 cacagatttt cgcacgatgt gaggctgctg ctaccatggc aaccccccgc gcatgtccta
     2581 atttcaaagc tgaattagga ttgttatgca taaatatctg ctcaatagca acctcagttg
     2641 gggaatagtg atccattagc tcacatattc catcatatat ttgcaataat ttatgactta
     2701 attccacatc gtttgatgtg cgaatacaac cgctatcgat gtacgcgatt ttacggtcag
     2761 attctttaat caatccatat cctgtaaccc gtgagcctgg atctatccct aatataatcg
     2821 tcattaattc attgattcta atattttttc agaaaattca gcattgctgt aaacttcttg
     2881 gacatcatcc aaatcttcca gcatatcaat cagtttaatt aagctttcag ccgtttcatc
     2941 actaattgga accaaggttt gtgcacgcat agttaaatga gactgctcta cctccaaacc
     3001 ggcatcctgt aaagcattta agacggtgtg ataagcttct acaggggtaa tgatctctat
     3061 ctgactgtcc tctacagcaa catcagaagc gccagcatcg attgcaatct ccatcacctt
     3121 atcttctggc tgattagaag ccatgagaat ttcaccttga ttagtgaaaa gataagatac
     3181 tgaaccatca gtgcctaaat tacccccatg ttttgaaaaa gcatgtcgaa cttccgatac
     3241 cgttcgattt ttattgtcag acaaacaatc taccaatata gcaacaccac ctggcccgta
     3301 accctcataa cgcatagcta tcattgcttc gccatcagcc ccccctaccc ctcgcttgac
     3361 agcattatcg atggtatcgc gcttcatatt ggcattcagc gcttttttaa cagcatctct
     3421 caaacgtgga ttacttgact catctcctcc tcccatacgt gcagctacag taatttcacg
     3481 aattaattta gtaaatattt tccctctttt ggcatcctgt acgcctttac ggaatttgat
     3541 attagcccat ttactatgac cagccattac atcacctcta aatgaatttt tatttggaga
     3601 ttatacaatg ctctaacacc aaaaagaatg actaaagcca tcgatgctct cgacaatttg
     3661 aacttttttg gactatgcta gcaccataaa aaaatcttga aggagattga actgtgtatt
     3721 tgcaaggatt tggtaattat caccacagcg aggctgttaa aggagcatta cccacaaatc
     3781 aaaactcacc gcagcactgt agcttaggac tttacgcaga gcaattgagt ggaacctcgt
     3841 tcacccgtcc ccggcataat aatcttcgaa gttggctata tagaatactt cctactgtta
     3901 cccagggaac gtattacccc tatgagttta atgttatgca accttttgtt gatgagttgt
     3961 cacccaatgc catgcgttgg tcacctcttt ataacagctc tcaaattaaa tgtgattttg
     4021 ttgaaggact atttcatatt gccggtagcc cgttagttaa tacctatact tattattgca
     4081 accactccat gagcgataaa tatttcgcca ataatgatgg tgagttatta tttgttccct
     4141 atgcaggcga gattcatctg catactgaat ttggcaaatt aatactatct tccggatcga
     4201 tcgcagtgat acctcgtggc gttaaattta aagtggaagt aatcagcaag gaggcaaaag
     4261 gttatctttg tgaaaatagc ggaaatccct taaccttacc tcagttaggc cccattggag
     4321 ccaatggttt ggcaaaccca agacattttc aatatccagt agccgcattt gaaaactctg
     4381 gtggcgagca tactataatc tgtaaaaacc agaaaaaatt atggtttact gtatgcaacc
     4441 actctccttt aaatgtcgtc gcctggcatg gcaattatgc accatattgt tatgatctca
     4501 gtttgttcaa tacaattaac acagtcagtt ttgatcaccc tgatccttcc atattcactg
     4561 tattaacttc agaaagcgaa atacccggtg tttctaactt ggactttgtt attttcccac
     4621 ctcgctggat ggttgccgaa catactttta gaccgcccta ttttcataga aactacatga
     4681 atgaactgat gggacttgtc tatggtgaat atgatgccaa gaaggaagga ttcataccgg
     4741 gtggtatcag catccataat tgcatgactc cacacggacc tgattatgaa tcttacgaaa
     4801 ttgcagcgtc gcaggatcta aaaccaaatt atatcaactc cctcgccttt atgtttgaaa
     4861 ccaaagacta ctggcaagta actgagcaag cttatcggca tcccagcaga caaatggatt
     4921 accttaattg ttggcaaggc tttaaaatag agtttagtca ataaattgac tgctttttaa
     4981 gatgtaaaat tgacggtaat taattaattt ggtttcagtt gggtcaaatt tgacccaact
     5041 tgtgtgtaat taattaactg gttaaatcaa aacaaatcct cttgtgttag tccaacccgc
     5101 tctaacaaag ttctaagtgt cttcaaagct tcaacttgaa tttggcgaac gcgttctcgg
     5161 gtcaaatcaa tttccttacc aacgtcttcc agtgtcgctt tttcaaaacc tcttaaccca
     5221 aaacgtcttg cgataacctg ctgttgattc tcagttaatt tatccagaag agattctata
     5281 tgctgcctta aattctcatt ggttagcagc tctgcaggat ttaaactgtt ttcatcagca
     5341 attgtatcta ataaagattt ggtttcatca aatccaattg gagtatcaac tgaagcgact
     5401 ttatcattca atcccaataa tttttggaca tcctccaaag gcttatccac catttctgct
     5461 atttcttcag gtgatggttc atgatccagt ttttgcgtca attgtcttgc cgctctgaga
     5521 taaacattaa gttctttcac gacatgaata ggcaagcgga tagttctcgt ttgattcata
     5581 attgcgcgct caatagtttg cctaatccac caggtcgcat aagttgaaaa acggaatcct
     5641 cgcttggggt caaatttctc aacagacttc attaaaccca ggttaccctc ttcgattaga
     5701 tccagaagag gtaatcctct attaagatag cgtctggcta ttttaaccac caggcgcaaa
     5761 ttcgactcaa tcattttctt gcgtgcttcc atatcacctt ttaaagcaag agtcgcataa
     5821 tggatttctt cttcagccgt tagcaaagga gaaaaaccaa tttcacccaa gtaaagttga
     5881 gtcgcatcta aagccttggc aatatttttc ccgcgctgga atgaaggaaa agcttcatca
     5941 tcagtaaact ctggaagttc atcaagctct tcttccattt tttcaatatc aggttcaata
     6001 tcctcttctg acaacagaga gtcatcatct ggctcagacc attcctcttc tttaaattcc
     6061 ttgtccttaa ttggctcttc ttcatcttgc attgtatcac ctatatgttt agctatgcct
     6121 tgaaatagtt ttttacttct taacaagcct attgttgcta taactatgag ttatatgtca
     6181 acctttttgt agataattca atggattaac tggtacccct gctcttctaa tttcaaaatg
     6241 aacaccccag tatgatctat ctattaaacc agcttctgct attacctgcc ccgcactaac
     6301 acgctgccct tctgtcacca gatttctggc attgttaccg tatgcagtta aatattcatt
     6361 actgtgttta ataatgatta aattaccata cccaggcaac ccgctgccag cataagccac
     6421 aactccactt gctgaagctc taactttgtc tcctctgtta catgcaatat ttatgccttt
     6481 tttcccctga gaaggaatga atgttgtaac aacacgacca gtaactggcc acagccaacc
     6541 agaggaagaa cgtacattat aagctggaga ataaataatt ttttgttttg gcttatgggt
     6601 aatatgtaag ttagctctgg gagctgaatg aaagtgattt gattttggca cgatgccttt
     6661 taaacttaaa acttgtccta ctcgcaagga ataaggtggc cttaggtgat tgatgatcgc
     6721 aagccgccta taatcagtat cataacgaaa tgctatggca taaagggtct cacctgatcg
     6781 caccgtatgg aatttctgat attttgaata aggctgccat tttagctcgg taacaggagc
     6841 caaatcactt cttgtcccac aaccagcaac acttaagata aaaaaaatac aaattaattg
     6901 tttgcgaaat aggcgcatca ttatctcatt tattatttat taattttcta tgcaaatttc
     6961 gttaagcaag ttagataaat gatcaaataa cttataatga gtcatgtcta aatgaagcgg
     7021 tgttatagaa acatgacctg ttttaattgc aaaaaaatca gtaccagcac cagcatctgc
     7081 ttctataccc ggtggaccaa cccaataaat tggccttcct ctgggatcat attctttaat
     7141 aataggctca gcactatgac gagttcccaa tcgcgtaacc tgcaatcctc ttatttgact
     7201 caaaggtaaa tcaggaacat taacatttaa tatagtctga gaaggtaatt tgttagcact
     7261 taattttata accaattgtt tggcaataat tgcagctgtt tcataatatt gtatgttgtc
     7321 tcctaccatt gagattgcaa tagcaggcaa ccctaaataa cgaccttcca tagccgctgc
     7381 cactgtacct gagtaaagta catcatcccc taaattggca ccctcattaa tccctgaaac
     7441 gactatgtct gctataggct ctaaaaatcc ggttaacgcc agatggacgc aatcagtagg
     7501 tgttccatcc acactataat aaccattgtc caattgttta actctcaaag gttgtgttaa
     7561 agtcaaagaa ttactggcgc cacttctatt tctatcaggc gcaaccactt taacttctcc
     7621 taaagtggac agctcattag ctaaaatttt gatcccgggc gccaaaaccc cgtcatcatt
     7681 gcttactaat attttcatgt ttaactctca ggggattcta accattttac aactgctaaa
     7741 tagtaagcaa gatcttcttg atttcttttc aatgaatctt gtaattcagc gttatccttt
     7801 ttactctgta tttcccgttg ctgattggca ataacctgct gcaaagccaa aattttgtta
     7861 ccaaactctt gtggattgta ttgtagttga taagcaagac cattcatcct cctgccaatt
     7921 gcctccagtt gctggcaact taatccatcg ggttgcgtgc cagggcacaa ttttaaagca
     7981 ttttgcagtt cggatgggtg ggaacgataa taggattcgt cttttttaaa ctgacatgaa
     8041 accaatccca aagttattaa tattatcact aagtacttat tcatacccgc cctcttgatg
     8101 taaactacaa aaagtcgata ttatcaccag tcagcaagta acgctatctt atgagaatac
     8161 catatgcttg attttagttt gaatcgtgat ccccaaacgg gcgagttata catagaaacc
     8221 tcattatgtg ggaagccttt actgactacg ccgcaattaa acaaaggaac agcatttact
     8281 caggaagaaa gaaaagattt cggtctttta ggcaagttac cacaccgcgt agaaactctt
     8341 gatgaacaag taaagcgtgc ctatcttcaa tattcaagtt acacgacacg tttgcagcaa
     8401 catatttatt taaataattt acatgataaa aaccaaatag tattttacaa actgctaagt
     8461 cgtcatttgg gggaaatgct tcccatcatc tacacaccaa tcgttggtgc tgcagccaag
     8521 aggtttagtc atgaatacag acagccacga ggattgtata ttgctcattc ggataaaaac
     8581 cagcttgagg aaataattaa taaccgttcg aatccagaca tagaccttgt tgtagtgacc
     8641 gatggtgagg gagtcctagg cattggcgat caagggatag gaggaatgga tatacctgtt
     8701 gcaaaactca tggtttacag cttatgtggt gggattgatc caactcgcac attacccata
     8761 tttcttgatg taggaactaa caaccaagaa ttattaaatg accctatgta tctgggttgc
     8821 cgtcacccca gaataaaaag ctcagaatac gacgatttta tcaaaacttt tgtcaatgaa
     8881 attcataagc aatttccaaa tgctttttta cattgggaag actttggtcg aggcaatgct
     8941 cgacgaatcc tggatcaatt tcaagatgaa ttgtgtactt ttaatgatga tattcaggga
     9001 actggtgcag taactctggc agcaattttg gcagcatgtg atgtaactgg actaccctta
     9061 catgaacaca gaatcgttgt gtttggagct ggctcagcag gtactggtat tagcgaccaa
     9121 attgttgatg cgatggtgaa aagcggattg agcctaactg atgcttatga cagattctgg
     9181 ttaatagaca aacagggttt attacttgcg actgaccaag agcttactga tgctcaaaaa
     9241 ccttatgcat ggaatcctat agatatacaa tcctgggaaa ttaataataa acaacatcct
     9301 tcgtttactg acactatcag gcatgtcaaa cctaccattt taattggctg ttcagcgcaa
     9361 acgggtgcat tctcacaaga tattgtggaa actatgtcga cagcctgcga gcgaccaatc
     9421 attttccctt tatcaaatcc tgatgaaaag tgtgaagcac agccctcaga tatattggca
     9481 tggagtaaag gacgggcatt aattgctact ggaactgctt ttgcccctat tgaatatcag
     9541 aaccgaatgg tgcaaatagc tcaatgcaac aatgccttgg tattccctgg aattggattg
     9601 ggcgttttgg cggtaagcgc ctctcgttta accaaagata tgatattggc cgccgcccag
     9661 actttaagta aatttgcgcc aagtaaaaag gacagctttt tgcccctttt accttcatta
     9721 gacaatgcgc aaattgtagc caaagaaata gctattgcag tagctcagtg tgcaatagac
     9781 tcaggttatg ctcaaaaaaa tcaagacaag gatctacctc gattaattga tgaactcttt
     9841 tgggagccta gatatctgcc ttttagaaaa aggggataga gcgaataaaa taaagctcta
     9901 tgactatatt attgaaaaat aaattttctt aattcttagt ctgaggggtt ggcaaaatta
     9961 atcgttacat tttttatagt cccgctctct ggctcccagg aacgagcgta tttaaagacc
    10021 attttggttt gagtgggata ggttttacct ttgttcaaat taaaagtaaa tagcatttta
    10081 ccgcctgcac ctatcaaatt agtcttgggc ttttcataaa tgctattact taaggtaagc
    10141 aaatttttgt caaattctac tacagaccat tgataacctg tagttggatt agctgcaaga
    10201 ctgacaacga aactgggatt ttttgagctg acattgatga caacctcgtt atttgcatag
    10261 gccagagcag agaaacccat aagtaatacg ctgaataatt ttttcatatt tttccctcac
    10321 aaagcttctt acacgctcac cattatgggt tacgatttac gttaatctgt tttaatttcc
    10381 cataatcaaa aattaattcg tattgaaaat caacaggaga tttttgataa atccaggttt
    10441 cgactgtatt agtaccataa cctatctgat aacccgccgc gttgtataat ggcacattct
    10501 cctcatagac taatttatca agtggctcac cacatttaga caaaacagtg tacttactat
    10561 ccccttcata gaccaattta tctccacacc gcattgcata cgcattagga tatacaatca
    10621 ttaaaaagac aatagccaac aaataattcc ggatgatcat tccctacccc ttgagaactc
    10681 aatgctcctt gcaacaaaat tttcaaaaag tctattttta tagcatagca cttggtatct
    10741 gattattcat cccaattgtc tctgtctcga tcggattcag gcaagaatcc acctacttga
    10801 gcatcccaca tggatttata taaaccatcc ttcgctaata actcttcatg gcttccatct
    10861 tcaattattt ttcctgcttc aaaaactaaa attctatcca tatgcaatag tgttgataat
    10921 cggtgggcaa taacaattgt agtctttgat tccatgagtt gccacaatga attttgtatg
    10981 gcttcttcgg ttagagaatc cagctgtgaa gtagcctcat ctaatatcaa aatcttcgta
    11041 ttttttaaaa tagcgcgagc aattgcaacg cgttgtcttt gcccccctga tagtttaatt
    11101 cccctttcac ccaccaaagt attatagcca tcgggtaaaa gtgaaataaa atcgtcacag
    11161 catgcctgtt ttgatgccat cacaacctca tcatcactcg catcagggtt accataacgg
    11221 atgttttcca tcagagttct atgaaacaat gtagtatctt gagggataag gctgatagct
    11281 gaacgtaagg aatcttgaga cacttggctg atgtcctgac catcaatcaa aattttccca
    11341 gattccacat taaataggcg taaaattaaa tgaataaagg tcgttttacc gcttcccgaa
    11401 aatcccacca aaccaacacg ttgtttaggt ttaatgatca ctgatttatt ttcaaatact
    11461 ttcttaccct ggttataatg aaaagttacg ctatcaaact ctatttttcc ttctgtaaca
    11521 atcaatggct tggcatcagg agcatcttct acctctatcg gtgcactaag caaagcaata
    11581 gcttgtcttg ctatgcctat ctctcgaaag aactcggcaa tagcatggca caaataccaa
    11641 atttgagtca tgattgaaaa cgatacattg aatataaaga ccaaatctcc tgttgtaatg
    11701 agctttttat tccaaaaagt caacaagaaa tacaccatag ccactaacat cactgtgact
    11761 ggaatatcga ggtacaaacg aaatatgttc atataaatcg acagcttttt attactttgt
    11821 acctccatat tctgacttgc tgaaatataa gccagctcat cgcgagaacg agcgaaaagc
    11881 ttaaccacac tggaattgct caaactgtca actattttac cgcttaactg acttttatcc
    11941 tctgcgtttt cttttgcata ttgatcaacc tttttagaca atctatagct gatataaagc
    12001 tgaaaaataa tccaacttaa caaaataact gcaaaccagt agttaatggt acacatcaaa
    12061 atcaatgcca caatgataga agcgaatgcc gcgatcgcat accaggttat gatattgaat
    12121 atggaatcaa gagcacgggg taaatcgttg actttgttag ctaaaccacc cgccatttga
    12181 ttggaaaaat aatgataaga ctggcgactc aaatactcca tgacagacat tctgacatca
    12241 gcctgaaatc ttggtatcac ataaccttgc caccaatttt gcagacgtaa aatcacaatt
    12301 aaggcgaacc aagcacctcc acctaaccat aaggccggag caactgctga aaaaatattt
    12361 tcctccccgg agtgcccccc aagagcatca atgatcattt tcaaagaata gggaatagca
    12421 ttattttcaa gcaccatagc aatcggtgct acaaaaaaca cagtaaaagc caaaggctgt
    12481 ttctttataa aatgccagaa aaacctaccc agtgtttttg gcaactcttc agattttttg
    12541 ttatagatat tcataaacca taccttcaat gaattttgac aaggttcgat tattctaacc
    12601 taatatttgc taattggaga atttatggct aaaaaacaat tatatgccct atcaattcag
    12661 ctattctccc agatctacat tgccataaaa taaaattcgc ccttcaaaaa ttgaaaatat
    12721 aaaatttaat cttgatctta aaccttataa aggcgatcat tttttcattt gtaagttaga
    12781 ggagtacgtg tggaacacga aacgatggaa tttgatgtta tcatagtcgg tgcagggcct
    12841 tctggattat ccgctgcaat caaattaaag caactggctt tagcttcaca aaaagaaatt
    12901 actatttgta tactggaaaa aggagctcag gtcggcgctc atattctttc tggtgcagtt
    12961 ttagaaccca gaagcttaaa agaattactg ccagagaatt ggcaacaggc cccgctagat
    13021 actcctgtca acaaagatct gttttatttt ttgactcaaa ataaatcatt taagctccct
    13081 actccaaaac ctatgcataa tgaaggaaat tttattatca gtttgggaga gctatgtaag
    13141 tttcttgcag aacaagcaga agctctaggc tgtgaaatat accctggatt tgcggcagca
    13201 gaaattctct ataatgataa aaatcaagtc ataggtgtag ctactggcga cgtaggaatt
    13261 gatcgacaag ggaaaaaaac tgataattat caacctggca tgcatttatt agccaaacaa
    13321 actttatttg ctgagggatg tcgcgggcaa ttaagtcaaa cattaatgaa tcgctatcat
    13381 ttaagagatc attcgcaacc acaaacttac ggtataggga ttaaagaaat ctggcaagtt
    13441 gatgcatccc ggcatgaacc tggaaaagtc atccatactg ttggttggcc tcttgatcat
    13501 gcgacctatg gaggctcctt tttgtatcat ctttcagagc agagagttgc tataggtttt
    13561 gttgtgggac ttgactacaa gaacccatgg ttaaatcctt ttggagagtt tcagagattt
    13621 aaaactcacc cccttattaa atctgttcta acaggcggtg agcgaattgg ttatggagcc
    13681 agagcactta atgagggagg atggcaatct ctccctaaac ttacctttcc tggtggcgcc
    13741 ttgattggag atgctgccgg tttcctaaat gtgcctaaaa ttaaaggaat tcacaccgca
    13801 atgcaatctg gaatgcttgc tgcacaagct tgctttgatg cgctaagtga ggaaaagagt
    13861 ggtcaatttg agctaagcgc ttactctgaa aaaatcaatc aatcctggct aaagaaagag
    13921 ctatatgcag tgcgaaatat tcgaccggga ttcaaatacg gtttatttcc cggcctaatt
    13981 aatgctgcct tggaaactta tgtaactcga gggtattctc catggacttt aaaaaatcat
    14041 gcggaccata aatcgttaat tccagcagaa aaagctaaaa aaattaacta tcccaagccc
    14101 gatggcgtgc tcacatttga taaactctca tctgtatttt tatcaaatac atttcatgaa
    14161 gaaaatcaac cctgccatct caagttaaaa caacctaagc tggccattga agtgaactta
    14221 aaccaatacg cttccccaga aagccgctat tgcccggcag ctgtttatga aataatcgaa
    14281 gatgttgaaa aagggcctcg attgcaaatt aatgcccaga actgtattca ttgtaaaacg
    14341 tgcgatatta aagatccacg tcaaaatata gtttggcaag cgcctgaagg tgggggtgga
    14401 ccgaattact caggaatgta accaatcaaa cttaaataat gtttttgggt agagtcctgt
    14461 aattattagc agggatgtgg atataaaaca tgaaaataag gattattaca tgtaccagcc
    14521 ttatctttta tttgccattt
//
LOCUS       DAXLDT010000074        14491 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXLDT010000074 DAXLDT010000000
VERSION     DAXLDT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.074"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(92..304)
                     /locus_tag="ACF2SP_001810"
     CDS             complement(92..304)
                     /locus_tag="ACF2SP_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359594.1"
                     /translation="MKSTNNMQKKETDKPTSAQAQKKEVRRNNAPDFIPGASPYYNLY
                     CFFYKLCHGEDTSTQHQPNTTIKHGR"
     gene            514..912
                     /locus_tag="ACF2SP_001811"
     CDS             514..912
                     /locus_tag="ACF2SP_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359595.1"
                     /translation="MNCFPFFAKKNKKNNEEKAEKTLSVFPAGTSINKTTVKEICFTD
                     IFCETHPFVWYYYPEHNLAIWTSRLAGTTQEYPADYLLNQLAKIPGITIDQGDAAIKD
                     VSTFLQDHQMIPIRNSESWRYIKSSSFSMS"
     gene            1111..1773
                     /locus_tag="ACF2SP_001812"
     CDS             1111..1773
                     /locus_tag="ACF2SP_001812"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFD2359596.1"
                     /translation="MKPTDKKHWSKIEYLHQSVKNPNIHIKGKHSYYSDAWTGSFEET
                     VVRYLYGDEYSLKTWQPQWQIDQLYIGDYVCIAAEVIILLGGNHNHRADWFCLYPFAD
                     KYVEAYQGKGDTIIEDGVWLGMRAVIMPGVTIGEGAIVAASSIVTKDVEPYSIVAGNP
                     AKPVKKRFAENVVERILALDIFSWSEAKFNLLKDYICGNNIDKLEQASRNYDQNLSIG
                     SK"
     gene            1770..2648
                     /locus_tag="ACF2SP_001813"
     CDS             1770..2648
                     /locus_tag="ACF2SP_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359597.1"
                     /translation="MISIRQLRLGDESTLETFLKSHADESMFLRSNLYHSGLSYHDKP
                     FHGDYFASFDNNKKISGVLAHYWNGNVIVQCPDFNVLTALVGAFQKTATREIAGILGE
                     DQQADVVMKQLQLEHVDYAVNYPDGLYALNLQNLAIPDNQDRYQLKVAKDCDKCLLYE
                     WLRAYQIEALGAEESSSILKEQVESEVKNTLEGNDRWVLEENGIPVSLCGFNARLPDI
                     VQIGPVWTPVTLRGRGYARTVVALSLSAAQKSGVNRAVLFTNNQAAVRAYQAIGFNKV
                     GNYRLAILKKPILLTR"
     gene            2701..3996
                     /locus_tag="ACF2SP_001814"
     CDS             2701..3996
                     /locus_tag="ACF2SP_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2359598.1"
                     /translation="MDMDIKHKPLKRELSLFGATMMGLGSIVGTGVFVSIGIAAGVTG
                     PSVILAIALAALVATCNAFSSAQLAANHAVSGGTYEYGYRYLHPAFGFTAGWMFLCAK
                     SASAATAALGFAGYFIHLSGLETVPVIPIALAVSITLILLVLSGLRRTNTVNIIIVMI
                     TLITLTVFIISGLPDFMQRGTANLRPFFPVSKQEGLSHFFYATALMFVAYTGYGRIAT
                     LAEEVKNPGKFIPAAIIITLMVSAVLYILVGLVAIGAVGVEKLAQVTETNATPLESAA
                     QAIGIPGLSVLVSIGACTAMLGVLLNLILGLSRVALAMGRKGDLPKIFCYVSETRHVP
                     VSSVIGVGVVIIALVLTGSVETTWTFSAFTVLIYYAITNLAALHLPKEEQLYPPLFAI
                     GGLLSCLFLAFWVPIGVWITGISLLVLGLLWKVVANRFL"
     gene            4078..4944
                     /locus_tag="ACF2SP_001815"
     CDS             4078..4944
                     /locus_tag="ACF2SP_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HFD2359599.1"
                     /translation="MLDGIMVLWFILLMLSLAFIAYDVREMPIDWVQKLGWVLVVAYT
                     GPLGLFFYFLTCRSPGKGLHALYTKAQWKQAVNSEVHCLAGDATGIIFAAIFLSFIDF
                     SNGIELILEYISAFFFGWVIFQAGMMRSMYSSYGEALRKTFFAETISMNFVMMGMIPV
                     MMILMNTLDYGRNPAHLQFWFIMGIATVVGGFLAFPINNWLVKKKLKHGCMTLPEGDE
                     HIEGHMTHHHGGHEEHLQAQAERIDSKVIHGHTHEMGSLPLNIQLKYIGISFGILLII
                     VLIMAFFVPLKI"
     gene            complement(5005..5157)
                     /locus_tag="ACF2SP_001816"
     CDS             complement(5005..5157)
                     /locus_tag="ACF2SP_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359600.1"
                     /translation="MFNLKRNLIKLGTLSISIALLAACHTVKGTVTGAKQDIKATGKA
                     ISRTMQ"
     gene            complement(5398..5880)
                     /locus_tag="ACF2SP_001817"
     CDS             complement(5398..5880)
                     /locus_tag="ACF2SP_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFD2359601.1"
                     /translation="MWLDYLHKINSDPNLLYLFIRTFIIYVFAIFIIRVGNTRYNFET
                     AFDYIFVFVIGAVLSRAINGNSTLISAMAGSSLLIFLHWVFAICSFYSHGFGKLVKGK
                     SVLLIQDGQLIWSALKRHQVTEEDLRQVFREKLNASDLTTVKEARLERTGRISFIIYK
                     "
     gene            complement(6093..6986)
                     /gene="prpB"
                     /locus_tag="ACF2SP_001818"
     CDS             complement(6093..6986)
                     /gene="prpB"
                     /locus_tag="ACF2SP_001818"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFD2359602.1"
                     /translation="MSNFRYSQGKKLREALMKEKPLQVVGTINAYAALQAKRAGFHAI
                     YLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTI
                     KEMIKAGAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
                     DALANDGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVT
                     PLFTLEELKEVGVSLALYPLSAFRAMSAAAEKVYDTIRKNGSQKDILAEMQTREELYQ
                     VLNYHFYEDKLNELFMKEKTT"
     gene            complement(7295..8029)
                     /locus_tag="ACF2SP_001819"
     CDS             complement(7295..8029)
                     /locus_tag="ACF2SP_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFD2359603.1"
                     /translation="MLNKLMWLTGKAQEDWIEKILHLRQKIWPDYQRIKNDTLFKLYS
                     HYPLYQWLSHYNGEVVAVINSLPVALDKSLDNLPQEGLNWVLSHLESTTPLTQSNYLA
                     AVSVTVNPAYQRLSLSKLLLNRLKASAQEAQYKALIVPARPSQKENYPLISLEDYLKW
                     KRVDNTIFDPWLRVHAQLGGKIAYPCQQSRVITANIAQWEQWFGHPLLQTGQYLVKGG
                     LNPVTINKEKDIGIYIEPNVWVIHDL"
     gene            complement(8022..8723)
                     /locus_tag="ACF2SP_001820"
     CDS             complement(8022..8723)
                     /locus_tag="ACF2SP_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3865 domain-containing protein"
                     /protein_id="HFD2359604.1"
                     /translation="MIREYMDKRNNQFTTEILNYFSDLKKFSTSYLEWLTMEHYQFSL
                     NNTRFLMNSHIQTKSLSDPGVSEELLANYNEEKNHAQMYRHALKKIGLDVTNRIPYVA
                     TRQFFMRLEALIEINASKTLGVMYATETAAIFEHILFKEISAELCQRKRLDFENSRIK
                     QFHDLHLDGVEQAHKDELGKFMDHTLAQEHVDPNIFLQEKEIMDGVYAAIDIMEDWWS
                     GLINAKAHLLEQAHA"
     gene            8829..9737
                     /locus_tag="ACF2SP_001821"
     CDS             8829..9737
                     /locus_tag="ACF2SP_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2359605.1"
                     /translation="MINFSQIQVFIAIVESGGFIAAGEKMHRSQPAITNAIKKLESSL
                     GFELFDRKVYRPQLTAKGKAFYQKAREVAAQFMQLENSAETLRSGVETQFSIDLDIAL
                     PLPGYAPLIQSFIRQYPETCFSLSNNTFVRCLERLERRECHLCIAASHIRSPQIEYLR
                     MPSIRMIPVASRDYYDQHKERINNPKLGIYCMQIMIAGTQQELYQLKIEPLSNSMPKW
                     FVGDMYIRKQLILSGMGIGRLPDHLVEKELAEGSLVQLNTRDYVAVTMDIYAMRLNNQ
                     EHGMVSNYAWQSLKSYADDTVSPDLK"
     gene            9882..10601
                     /locus_tag="ACF2SP_001822"
     CDS             9882..10601
                     /locus_tag="ACF2SP_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFD2359606.1"
                     /translation="MIDFKINSQPDDESCGPTCLHAIYQHYGLDISYEEVASQVESFL
                     SGGTIAPMLGKHALQLGFKATLYVNNLQLYDPTWFNSDGSSNTDVLREMLMQQMRYKR
                     NQYLARGTHAALDFLTLGGAIYFRCLTAEILKEYFGRKIPILTGLSSTYLYNCSREIF
                     NKEGKSRSDPIRGLPCGHFVVLCGYNQKHRLVVVADPYKKNPVSGDNYYTVSIHRLIN
                     AIMLGVVTRDGNLLIIEPKYT"
     gene            10637..11197
                     /locus_tag="ACF2SP_001823"
     CDS             10637..11197
                     /locus_tag="ACF2SP_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359607.1"
                     /translation="MPGTENHEFHTLTKSIVKNLLEFTGWNIAPGQKYIHSDPHQCYI
                     QHEGDQTLTIATLSSKVAKYKEEIRTGRTTSRSRDPVIDKCPEEYKLYRDFLDENCFV
                     RVRDSAKERLAAKFGVDSRGEVNAHFADLCELEMLGGQSVYEGFVNNYLVMSDKYIKA
                     HPQKDYDPDKINFLSSNPMGYTPKSS"
     gene            11305..11466
                     /locus_tag="ACF2SP_001824"
     CDS             11305..11466
                     /locus_tag="ACF2SP_001824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359608.1"
                     /translation="MGTVFSLPVRFQPDELGRIQLLWYRHLRVLGVDRVWALGLPEKG
                     IAIIDELNN"
     gene            complement(11503..11907)
                     /locus_tag="ACF2SP_001825"
     CDS             complement(11503..11907)
                     /locus_tag="ACF2SP_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD2359609.1"
                     /translation="MNNKKIGEFAKLCGVGIETVRYYQRQGLLHVPLLNKELGAEKIR
                     RYGDDDISRLRFILLAKKAGFTLKEIKELLEFDAKNDRERVRDMAEKRIEKLNLKINE
                     LTEARNRLSLLVTKCIETSSSPCPILMTFEKS"
     gene            11977..12720
                     /locus_tag="ACF2SP_001826"
     CDS             11977..12720
                     /locus_tag="ACF2SP_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HFD2359610.1"
                     /translation="MNHTSIKKAVLYRMVIEHHVCPYGLKARDLLRRQGYEVEDNWLT
                     TREQVDVFKDKHNVKTTPQIFINDERIGGYDDLRHYLGKKVRDPKEKSYRPVIVLFAI
                     TALMAMAVSYASFANPFTPHVLSWFIAFSMAVLALLKLQNIESFSTMFLNYDLLAKRW
                     VPYSYIYPFAEGLAGILMIAGALNWLSIPIALFIGTVGALSVFKAVYIDKRELKCACV
                     GGDSNVPLGFISLTENLMMIGMALFMLLM"
     gene            12919..14010
                     /gene="ceg2"
                     /locus_tag="ACF2SP_001827"
     CDS             12919..14010
                     /gene="ceg2"
                     /locus_tag="ACF2SP_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0059 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359611.1"
                     /translation="MSKEIKKNLQNNNSAFVYLRSTGLATNSLSSILQYMTRIIVLTE
                     SAGIALGILGVLFSVIGFVSSTIARIFDPTASKLSRVMSAGILIAAAAGAIPLIYLGT
                     LFPAMWVYLGIGIMGFGVLNSSYYLYSDVRKENALINQYKDNPEQYVESIKEKRQAKY
                     EKLLNTRLDDKQARVLLVFKLYQLDKKLAVAKQEGLARLYDIVCHRKHTRSLERFIDL
                     GTSLTSLIVGVSVLFWGIAAWPIGPILLGISLAVFSVSLSKSAMQFYRKVNKNNALLK
                     EVLSNDRYVSLIDANLDNPYDIEEMGTVEKTQEKVCTRELSQESVLSKDQEHKEHYTK
                     LFQPAHKTALRPNDEETDELSCKSVLLQR"
     gene            14052..14370
                     /locus_tag="ACF2SP_001828"
                     /pseudo
     CDS             14052..14370
                     /locus_tag="ACF2SP_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870088.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         4323 a         2653 c         2903 g         4612 t
ORIGIN      
        1 agccagtttt taaaaactta ttttagggag ccataactaa tgactcccta tacacagatt
       61 gttatcaaat ctagttatgt caaaaagaca tttatctccc gtgcttaata gtagtgtttg
      121 gttgatgttg tgtggatgtg tcttcgccat ggcaaagttt ataaaaaaaa caataaagat
      181 tataataagg tgatgcacca gggataaaat cgggggcatt atttcttctc acttccttct
      241 tttgtgcttg tgccgaagtt ggtttgtcag tctctttttt ttgcatatta tttgttgatt
      301 tcataaatct tatccttgat taagaaaata gattgaattt taagtatcgt tcaaaccagg
      361 aaagagagtt ccggcttgtt tacaaatgaa ccgttttatt gctcatggct tggtgattat
      421 agttcagaca ggtgatcaat tgtagtagtt gtcccagatg tgttatggtt atctgactga
      481 caggaatcga gatgccagaa caaggaatag ataatgaact gttttccttt ctttgcaaaa
      541 aaaaataaaa aaaataacga agaaaaagcg gaaaaaacat taagcgtatt tcctgcaggt
      601 acttctataa ataaaacaac ggtaaaggaa atttgtttta cagatatctt ttgtgaaacc
      661 catccttttg tttggtatta ctatccagaa cataatctgg caatttggac ttcaagactg
      721 gcagggacaa cccaagaata tcccgcagat tatttgctta atcaacttgc aaaaattcct
      781 ggaattacca tcgaccaagg tgatgcagcc atcaaggacg ttagtacttt cctccaggac
      841 catcaaatga ttcctattag aaacagcgaa agctggcgat atatcaagtc ttcttctttt
      901 tcaatgagtt aatgtagaga ttgaaagcag ggccttcttc ttaataatgg aagattttgc
      961 tggctcagtt tcgcaagtga tgcgcaaggg atattcaaat gatttctctc atcttattga
     1021 ttgcatagag cccacgcata atgaaacatt agaaagttat gatcataata atatacaatg
     1081 agatcattta aattcctaaa tgagcaaggc atgaaaccta ctgataaaaa gcactggtct
     1141 aaaatcgaat atctacacca aagcgtaaaa aatccaaaca tccatatcaa gggtaaacac
     1201 agctattata gcgatgcctg gactggtagt tttgaggaaa ccgtcgttcg gtatctttat
     1261 ggagacgaat acagtttaaa aacctggcaa ccacaatggc agattgatca attatatatt
     1321 ggtgattatg tttgtattgc tgctgaagtg ataatcctgt taggtggaaa ccataatcac
     1381 cgagcggact ggttttgttt gtatccattt gcagataaat atgtagaggc ttatcaaggc
     1441 aaaggcgaca ctattattga ggatggtgtc tggcttggca tgcgcgctgt tattatgcca
     1501 ggcgttacta ttggcgaagg agcaatagtg gctgcaagca gtattgtaac caaagatgtg
     1561 gagccctaca gcattgttgc tggaaatcct gcgaaaccag tcaaaaaaag atttgcagaa
     1621 aacgtggttg aacgaattct ggccttagat attttttcct ggagtgaagc caaatttaac
     1681 ttattaaaag actatatttg cggcaataac atagataaat tagagcaggc cagcaggaac
     1741 tatgatcaaa acctttctat aggaagcaaa tgataagtat caggcaatta agattaggtg
     1801 atgaatccac cctggaaact tttttaaaat ctcatgctga tgagagtatg tttttacgca
     1861 gcaatctcta tcattccggg ttgtcttatc atgataagcc ttttcatggg gattattttg
     1921 cttcatttga taacaacaaa aagattagcg gtgtattagc ccattattgg aacggcaatg
     1981 ttattgtgca gtgccctgat ttcaatgttt taacagcact agtgggtgct tttcaaaaaa
     2041 cggctacccg agaaattgca ggtattctcg gcgaagatca gcaagccgat gttgtcatga
     2101 aacaactcca gctggagcat gtagattatg ctgtgaatta cccggatggg ttatatgcac
     2161 ttaatttaca gaatctggca atacctgata atcaagacag atatcaactg aaagtcgcca
     2221 aagactgtga taaatgttta ttatatgaat ggcttagagc ttatcaaata gaagccttag
     2281 gtgctgaaga gagcagttca attttaaaag agcaagtaga gagtgaagtg aaaaatactc
     2341 tcgaaggcaa tgatcgttgg gttttagagg agaacggcat ccctgtctcg ctatgcggtt
     2401 ttaacgcaag actaccggat attgtccaaa ttggaccggt atggacacca gttacgttgc
     2461 gggggcgagg atatgctcga acagttgttg cgcttagtct tagtgcagcc caaaaaagtg
     2521 gagtgaatcg agcggtatta tttactaata atcaagctgc cgtacgagct taccaggcaa
     2581 ttggttttaa taaagtaggc aattatcgat tggccatatt aaaaaaacca atactcttaa
     2641 cacgttaatt gtgaatacat gttaaaacag taaaagtatt tattgatagg aaataaaacg
     2701 atggatatgg atattaaaca taagcctttg aagcgtgaac tcagtctatt tggcgctact
     2761 atgatggggc ttggctctat tgtcgggacg ggtgtttttg tcagcatcgg aattgcggcg
     2821 ggtgtcacag gaccatccgt cattcttgct attgctttgg cagcacttgt tgctacctgt
     2881 aatgcattct ccagcgctca acttgctgca aatcatgctg ttagtggagg tacttatgaa
     2941 tatggatatc gttatttaca tcctgctttt ggttttactg ccggttggat gtttttatgt
     3001 gcgaagagtg cttctgctgc aacggcagcg cttggttttg ccggttattt tattcacctg
     3061 tcaggtttag agacggtgcc tgtcatacct atcgctttag cagtgtctat cacgttaata
     3121 cttttagtct taagtggact cagaagaaca aatacagtga atattattat tgtaatgatt
     3181 acattgataa ccttaaccgt ttttataata agcggattac ctgatttcat gcaaagggga
     3241 actgctaatc tgaggccctt ctttcctgtt tcaaaacaag aaggattaag tcattttttt
     3301 tatgccactg ctttaatgtt tgtggcttat accggttatg ggagaattgc aacattagcg
     3361 gaagaggtta aaaatccagg aaaatttatt cctgcagcga ttattatcac tctgatggtg
     3421 agcgccgtac tttatattct ggtgggtctt gttgctattg gagctgtagg ggttgaaaaa
     3481 ctggcccaag tgactgaaac aaacgcaact cctttagaga gcgcggcaca agccattggt
     3541 atccccggat tgagtgttct ggtctccatt ggggcatgca cggccatgtt aggggtatta
     3601 ctcaatttaa tacttgggtt atctcgtgtg gctttagcta tgggtagaaa aggagattta
     3661 ccgaagattt tttgctatgt atcagaaaca agacatgttc ctgtttcctc tgtcattgga
     3721 gttggagttg taattattgc tctggtgttg actggaagtg ttgaaacaac atggacattc
     3781 agtgcgttta cagttttaat ctattatgca attactaacc tcgctgcctt acatttgcct
     3841 aaagaggagc aattgtatcc acctctgttt gctatcggag gcttactgtc ctgccttttc
     3901 ctggcgtttt gggttccaat tggagtatgg ataacgggaa tcagtttact tgtcctgggg
     3961 cttctttgga aagttgttgc caatcgcttt ttataaaggc aagaaaaaaa gatgaatgta
     4021 ttctagaata aaaatgtatt ctagaataaa caagaagatc agagaaaagg aaccgcaatg
     4081 ctcgatggaa ttatggtttt atggtttatt ttactgatgc tctctttggc ttttattgct
     4141 tatgatgtga gggaaatgcc aattgattgg gtgcaaaaac tcggatgggt attggtcgtg
     4201 gcctataccg gccctttagg cttgtttttt tattttttga cttgcagaag cccgggtaaa
     4261 ggattgcatg ctttatatac caaagcgcaa tggaaacagg cggttaactc cgaagtgcat
     4321 tgtctggctg gtgatgcgac agggatcatc ttcgcggcaa tttttttatc ttttattgat
     4381 ttcagtaatg gtatagagct aattctcgag tatatttcag cctttttctt tggttgggtt
     4441 atttttcagg ctggaatgat gagaagtatg tattcaagct atggagaggc tttaaggaaa
     4501 acattttttg cagaaaccat atctatgaat tttgtgatga tgggtatgat tcctgtgatg
     4561 atgattttaa tgaacacatt agactatgga cgcaacccgg cacatttgca attttggttt
     4621 attatgggaa tagctactgt agtcggtggt tttctggctt ttccaattaa taactggttg
     4681 gttaagaaaa aattaaagca tggatgcatg actttgccag agggggatga acacatcgaa
     4741 ggacatatga cccatcatca tggaggtcat gaagagcatc tacaggcaca agctgaaaga
     4801 attgattcga aagtgattca tggacacacc cacgaaatgg gaagcttacc attaaacatt
     4861 caattgaaat atatcggtat aagttttgga attttattga ttattgtttt gataatggcc
     4921 ttttttgttc cattgaaaat ataatagaga tttttattaa atctgaacaa tgttggatta
     4981 aaataatcca acattgtttt cgtcttattg catggttctt gatattgctt ttccagttgc
     5041 tttaatatct tgtttagctc ctgttacagt ccccttgaca gtatggcatg ctgctaatag
     5101 agcaatgctg atactgagag tccctaattt gattaaattc ctttttaagt taaacataac
     5161 tcctccttta tgcttatgat ttaaatgccc gttttttact tgccgctgca tgttttggat
     5221 aagcggagtc acgcaggtct ttaaacgagc catgtaagaa tcaaataatt acgttaaaag
     5281 aattctttac ataaagaata gaagagtatt cttaattttc aagatacaat ttctatccaa
     5341 tgaaacagcc aggatttacg aaaaaagtaa tcgccagaat tcgctcttgt tacaaaatta
     5401 tttgtagata ataaaactga tacgccccgt tcgctccaaa cgagcttcct tcactgttgt
     5461 caaatcgctt gcatttaatt tttctctgaa aacttgcctc aaatcttctt cagtcacttg
     5521 atgtcttttc aaagcactcc aaatgagctg cccatcttgt attaatagca ccgatttccc
     5581 tttaaccagt tttccaaatc cgtgggaata aaaggagcaa atagcaaaga cccaatgtaa
     5641 aaaaataagt aaactgctcc cagccattgc agatattaaa gtagagttgc cattaattgc
     5701 acgacttaac acggcgccaa tgacaaatac aaaaatataa tcaaatgctg tttcaaaatt
     5761 gtatcgggta ttgccaacac ggatgataaa aattgcgaaa acatagataa taaaagttct
     5821 tataaaaaga tataaaaggt ttggatcgga attaatttta tggaggtagt caagccacat
     5881 atctgtttca gcaaatctct tatcccaaaa ctataaatag aattgctttt cttttgcaat
     5941 aactttatca aattttaaat ggattgcttt tgaatgcttg tctggataga gagttaatct
     6001 gttgtttata aacatcaatt tcggcagata atcctctatc caggcatata aaaccttatt
     6061 ttctcaaatt agttctgata attaattgat attcacgtcg ttttttcttt cataaagagt
     6121 tcattaagct tgtcttcgta aaaatggtaa tttaatacct gatacaactc ttcgcgagtt
     6181 tgcatctctg ccagaatatc tttttggctg ccgtttttgc ggattgtgtc gtaaactttt
     6241 tctgcagcgg cactcattgc ccgaaatgca cttaaaggat aaagagccag gctaactcct
     6301 acttctttta attcttccag agtaaataat ggagtgactc caaattcagt gatattcgcc
     6361 aataccggaa cattacattg ctcagttagc gcctgatatt cctcaagttt acgtactcct
     6421 tcgaagaaaa tcatatctgc cccggcctca atataagcac taattcgctc tagcgcctta
     6481 tttaaaccgt cattcgccag ggcatcagtt cttgccataa taacaaaatc aggatctgtt
     6541 ttcgcatcca cggcggcctt aatacgatct atcatttctt ctttttcaac caatgcctta
     6601 cccgggcgat gaccacatcg tttcgcttga acttgatctt ctatatgtac cgctgctgcc
     6661 ccggctttaa tcatttcttt gatagtccgt gcgatgctaa aggccccacc ccatcctgta
     6721 tcaatatcga ccaggagggg taaatcaacg gcactcataa tgcgccgcac atcttcgagt
     6781 acatcattta atgtggtaat gcccaggtct ggtaagccgt aagaggcgtt agctactcct
     6841 gctcctgaaa gataaattgc atgaaatcct gctcttttgg cttgcaaagc ggcataggca
     6901 ttaattgttc ctacaacttg caatggtttt tctttcatta aagcttcgcg aagttttttt
     6961 ccctggctgt atcgaaaatt tgacatttgg tttccttgtt ttacctaaga tgagaattta
     7021 tcaggactta cgaaaaatcc caatctcttt gttaaaaaaa gtttttaatt cggtcattta
     7081 gtttatctaa actccctcat taaaaacatt ttttgccttg atcttggggt ttttcgtaag
     7141 tctggtttat tttattttta cgtttttttg gatatttttt caaacttctg gaaatgcaca
     7201 gcaaaacgac atccaaagct tgaaaagcgt atagatcatg taccaaagac aagcttgttt
     7261 gcctgatgtc ataacgagag atggcaaaaa tcaattacaa atcatgaata acccaaacat
     7321 ttggttcaat gtatattcca atatcctttt ctttattgat agtgacagga tttaacccgc
     7381 ctttaacaag atattgaccc gtttgaagta acggatggcc gaaccattgt tcccactgag
     7441 caatattcgc tgtgatcacg cgtgattgct ggcatggata agctattttt cctcccagtt
     7501 gtgcatgaac tcttaaccaa ggatcaaaaa tagtattatc cactcttttc cacttcaaat
     7561 aatcctctag ggaaataagt gggtagtttt ctttttggct aggtctggct ggcactatta
     7621 gtgctttata ttgagcctcc tgtgcactgg cttttaaccg attcaataag agtttgctta
     7681 aactaagtct ttggtaagcc ggattcactg tcactgatac agcagcaaga taatttgatt
     7741 gggttaaggg tgttgtagat tccagatgag acagtaccca atttaatcct tcttgtggca
     7801 aattatcgag tgatttatca agtgctactg gcaaagaatt aataacagct actacctcac
     7861 cattatagtg agatagccat tgatataaag gataatgact atagagctta aaaagagtgt
     7921 catttttaat gcgttgataa tcaggccaaa tcttttgtcg taaatgtaga attttctcta
     7981 tccagtcttc ttgcgccttg ccagttagcc acataagttt attaagcatg agcttgttcc
     8041 aaaaggtgtg cttttgcatt aattaaaccg gaccaccaat cttccattat atcgatcgcc
     8101 gcatatacgc catccattat ttctttttct tggagaaaaa tattgggatc aacgtgctct
     8161 tgagcaagcg tatgatccat aaacttgcct aactcatctt tatgtgcctg ttctactcca
     8221 tctaggtgta aatcatggaa ttgcttgatg cgagaattct caaaatccaa acgttttctt
     8281 tgacaaagct cggcgcttat ttctttaaat aaaatatgct caaatattgc agctgtttca
     8341 gtagcgtaca taacgccaag ggttttggat gcgttaatct caatcaaggc ttcaaggcgc
     8401 ataaaaaact gtctggttgc cacatacgga atcctgtttg ttacatccag accgattttt
     8461 tttagtgcat gccgatacat ttgagcatga tttttttctt cgttatagtt agctagtaac
     8521 tcttcagaaa ctcctggatc tgaaagtgac ttggtttgga tatgagaatt cattaagaaa
     8581 cgagtgttgt ttaacgaaaa ttgataatgc tccatggtta gccattcaag atagctcgtc
     8641 gaaaattttt ttaggtcact aaaataattt aaaatttcgg tagtaaattg gttgtttctc
     8701 ttatccatgt attctcttat catgaagcct cccgtcaata ttaaaatcaa ttcatcaagg
     8761 gtttaccata actataaaga tcatataatt aaaatattat tttataatta tcttgataat
     8821 ttttagttat gataaatttt tcacaaatcc aggtgtttat tgcaattgtt gaatctggtg
     8881 gatttattgc tgctggagag aagatgcatc gttcacagcc ggctataact aatgccataa
     8941 aaaaattgga gtccagcctg ggcttcgaac tgtttgatag aaaagtatac cgacctcaac
     9001 tcactgcgaa aggtaaagct ttttatcaaa aagcaagaga agtggccgct caatttatgc
     9061 aactggaaaa tagtgctgaa acattgagat ccggagtaga aacacaattt tctatagatc
     9121 tcgatattgc tttaccattg ccaggatatg ctccgcttat tcaatctttc ataagacaat
     9181 atcccgaaac ttgtttttca ctaagtaaca atacttttgt tcgatgtctt gagcgcctgg
     9241 aaagaagaga gtgccattta tgtatagctg caagccatat tcgttctcca cagatagaat
     9301 atttgcgtat gccttccata cgaatgattc ctgttgcgag cagggactat tatgaccagc
     9361 ataaagaacg aataaataat cccaagttgg gaatatactg catgcaaatt atgatagctg
     9421 gcacgcaaca ggaattatat caactcaaga ttgagccatt aagtaattct atgcccaaat
     9481 ggtttgtggg tgatatgtat attcgtaaac aactgatttt atcgggtatg ggtattggcc
     9541 gtttaccgga tcatctggtt gaaaaggagt tggcagaggg ctctttggtt cagttaaata
     9601 ctagggatta tgtagccgtt actatggata tttatgctat gcggttaaat aatcaagagc
     9661 atggcatggt ctccaattat gcttggcaat ctctaaaaag ttatgctgat gacacagtat
     9721 ctcccgattt gaaataatca atctttatgt ttgcttgtta attccagcag tttggtggac
     9781 cgaatatttg aaaaacagga ggtgattcat tgacataaaa ccaagataag cctaaattta
     9841 aatcaaaaag acataaaatt gatctccagt tggatattat aatgattgat tttaaaataa
     9901 attcccaacc tgatgatgaa tcttgcgggc caacctgttt acatgccata tatcaacatt
     9961 atggcttgga tatcagttat gaagaggtgg caagccaggt agagagtttt ttatctggtg
    10021 gaacaatagc gcctatgctt ggtaaacatg ccttgcaatt gggatttaaa gccacccttt
    10081 atgtcaataa tttacaattg tatgatccga catggtttaa tagcgatggt tcatcaaata
    10141 ccgacgttct gagagaaatg ctcatgcaac aaatgaggta taagcgtaac cagtatcttg
    10201 ctcgcggaac acatgcggca cttgattttc tgactttagg gggcgctatc tattttcgat
    10261 gcctgaccgc agaaatttta aaggaatatt ttggcaggaa gattcccata ttgacagggc
    10321 ttagttcaac ctatctgtat aactgcagtc gcgagatatt caacaaggaa ggcaaatcga
    10381 ggagtgatcc tattcgcggc cttccgtgcg gacattttgt tgttctgtgc ggatacaacc
    10441 aaaaacatcg tttagtggtg gtcgctgatc cttacaaaaa aaatccggtt tcaggtgata
    10501 attattacac cgtatccatc catagattaa ttaacgcaat tatgttggga gtagtgacac
    10561 gtgatggtaa tttattgatt attgagccga aatacacgta gatgagtttt tatttttaaa
    10621 cgcttacaac aaacctatgc ctggtactga aaaccatgag tttcatacat taacaaaatc
    10681 cattgttaaa aatttgctgg aatttaccgg atggaacatt gcccctgggc aaaagtatat
    10741 ccattcagat ccccaccagt gttatattca gcatgaaggg gatcaaacct taactatcgc
    10801 gactttatcc tccaaggtcg ctaaatacaa agaggaaata agaacaggga gaaccacatc
    10861 ccgctcaaga gatcctgtta ttgataaatg ccctgaagaa tacaaactat atcgtgactt
    10921 tcttgatgag aattgttttg tcagagttag agattctgca aaagaacggt tagctgctaa
    10981 atttggtgtg gacagccgcg gtgaggtgaa tgcccatttt gccgatcttt gtgaattgga
    11041 aatgctggga gggcaatcgg tctatgaagg atttgttaat aactatttag tcatgtcaga
    11101 taaatacata aaagcacacc ctcaaaagga ttatgatcct gataaaatta attttctttc
    11161 ttctaatcct atgggctata ccccaaaatc aagttaattc aaagcaagta accctcttcc
    11221 catgccctgc tattgttatg tcgataaggt aagctcattg ctaaaatccg gttaaaggat
    11281 aatatcaccc aaacatccca caatttggga acagtatttt ctcttccagt tcggtttcaa
    11341 cccgatgaat taggacggat acagcttctt tggtataggc atttacgtgt tttaggtgtc
    11401 gatcgagttt gggcgcttgg cttgccagaa aagggaattg caatcattga tgagcttaat
    11461 aactagttat caataatttg attgctttct atttttatgg gtttaacttt tctcaaaggt
    11521 cataagaatg gggcaaggcg aagagctggt ttctatacat tttgttacta agagtgaaag
    11581 tctattccga gcttcggtta attcattaat ttttaaatta agtttttcaa tacgcttttc
    11641 agccatatct cttacgcgct ctcgatcatt tttagcatca aactccaata gttcttttat
    11701 ttcttttaaa gtaaagcctg cttttttggc taaaaggata aaccttaagc gacttatatc
    11761 atcgtcacca taacgcctta ttttttcagc cccaagttct ttgttaagca aaggaacatg
    11821 aagcagtcct tggcgttgat aataacgcac ggtttcaatg ccaactccac ataatttagc
    11881 aaattcacca atttttttat tattcattac ttgactccgt actatagtac gtactctata
    11941 ttactattta tagaccacta atcatagggg atagaaatga accatacttc aattaaaaaa
    12001 gcagttttgt atcgcatggt gatagaacac catgtttgcc cttatggtct caaagccaga
    12061 gatttattaa ggcgtcaagg ctatgaggta gaagataatt ggctaacaac ccgtgaacag
    12121 gtcgatgtgt ttaaggataa acacaatgta aaaaccacac ctcaaatttt tatcaacgat
    12181 gagcgtatag ggggttatga cgatttacgg cattaccttg gaaagaaagt aagagatcca
    12241 aaagaaaaaa gttatcgccc cgtcatcgtg ttgtttgcga ttaccgcttt aatggcgatg
    12301 gcagtgagtt atgctagctt tgctaaccct tttacaccac atgttttaag ctggtttatc
    12361 gcatttagca tggcagttct tgctttatta aagctccaga atatcgaaag cttctcaacc
    12421 atgtttttaa actatgatct cttggccaaa cgctgggttc cttacagcta tatttaccca
    12481 tttgccgaag gtttagcagg gatattaatg attgcaggtg cgttaaattg gctttccatt
    12541 ccaattgcct tatttattgg aactgtaggt gctctttctg tttttaaagc cgtttatatc
    12601 gacaagcgtg agcttaaatg tgcctgtgtg ggaggtgata gtaacgtacc tttaggattt
    12661 atttctctca ctgaaaattt aatgatgata ggaatggctt tattcatgtt gcttatgtaa
    12721 ttcaactagc aagcgtagcc cggatgaagt ggaacgaaat ccgggagtat gactgcgctt
    12781 gcatcagtgc tacgctagct atacactgtc aatatataaa aagttgatca tcgagctatc
    12841 gatatccagg gttgttaaag ttctcttaag acaaacctta tataatgcac attttttata
    12901 tttattccag ttctgccgat gagcaaagaa atcaagaaaa acctacagaa caataattca
    12961 gcctttgtct acttgcgcag tacggggctt gctacaaact ctctgtcgag cattctgcag
    13021 tacatgacac gcatcattgt acttacggaa agcgcgggca ttgccctggg cattctggga
    13081 gtgctctttt cagtcattgg ttttgtaagc agtacgattg caagaatatt tgatccaaca
    13141 gcctctaaat taagtcgtgt gatgtcggct ggtattctca ttgcagctgc agcaggggcg
    13201 ataccactca tctatctggg aacgttattc cccgcgatgt gggtatatct tggtataggc
    13261 attatggggt ttggcgtact taattcgtca tactaccttt attcggatgt gcgtaaggaa
    13321 aatgcactca taaatcagta taaagataac cctgaacaat atgtggaaag tattaaagaa
    13381 aagcgacaag ccaagtatga aaaacttctc aacacgcggc tcgacgataa gcaagcacga
    13441 gtgctgctag tttttaagct ttaccaactt gacaagaaat tagccgttgc taaacaagag
    13501 gggctagcta gactttacga catcgtatgc catcgcaagc acacccgcag tctggagaga
    13561 tttattgacc ttggcacttc tcttacaagc cttattgttg gggtgtcagt cctgttttgg
    13621 ggaatcgctg cctggccaat tggccccata ttgctcggta tatctttggc tgtattctct
    13681 gtaagcctat caaagtcggc gatgcagttt tatagaaaag ttaataaaaa caatgcgctt
    13741 ttaaaagaag ttctctcaaa tgatagatat gtatcactta ttgatgcgaa tctggacaac
    13801 ccgtatgaca tcgaagagat gggcactgtg gaaaaaacac aagagaaggt ttgtacgcgt
    13861 gagctttcac aagagtctgt gctctcgaaa gaccaagaac ataaagaaca ttataccaaa
    13921 ctcttccagc ctgcgcataa aaccgctctc aggccaaacg atgaagagac ggatgagcta
    13981 agctgcaagt cggttctact ccaaaggtag tggcactata atcgggttat agcaatctaa
    14041 ctctttgact ccgcaatcaa aagaggatta atgagtatgt ttttccaggc acaagagcag
    14101 caggtcatat cattgaacca cgtaagcaaa tggccaatgt gaccaaactc tcaggtattc
    14161 attttacggt tcatggttta agacgaactc ttatcactat tgccgagggg ctggatatct
    14221 cggcttatgc tttaaagcgt ttaagaagac ccatgcaaca aattactgat tattttatga
    14281 aggtgtatgg gtattcaacc ttcggcaacc ctgttagaaa tccaaccgca acagggagta
    14341 aaatgtgaaa aatggaatca aaattcataa cgatttattt gattaaagaa catttattta
    14401 ttatgtggat atcattcgct ccttaaaagc tctcatccct ttgaagattg gatctgcaat
    14461 cttttctggg aagtggtttg gtaattgtgt t
//
LOCUS       DAXLDT010000075        14397 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXLDT010000075 DAXLDT010000000
VERSION     DAXLDT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.075"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1013
                     /locus_tag="ACF2SP_001829"
     CDS             <1..1013
                     /locus_tag="ACF2SP_001829"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFD2359612.1"
                     /translation="MKYRKLGKVGPDVSALGFGCMGLSEFYGKPARLDDAVKIIRNAH
                     DQYQVNFFDTADIYGKGKNEELVGKAIKPFRNQIILATKCGVVRTGSNDEMSVNNSSA
                     YIRTACEKSLKRLNTDHIDLYYLHRYDHQTPIEEVMGIMHDLIDEGKIGYIGLSETDA
                     DTIRAAYTVVKDKLVAVQTEYSLRVRAPATAVLGTCRELGICFVAYCPIARGFLSGKI
                     KKPNLLGTNGFDFRTNVPQFQPENFDQNLGLVNELETIGEKTGYTPAQLSLAWLLAQG
                     EDIIPIPGTSNPQHLAENMRSIDIFLTPEQMKDLENAYKNNPVAGKRLSKELMAAFHL
                     KE"
     gene            1121..1933
                     /locus_tag="ACF2SP_001830"
     CDS             1121..1933
                     /locus_tag="ACF2SP_001830"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HFD2359613.1"
                     /translation="MDYYFTKGKPQLVNMKTPIYLVTQFQQLMDLMQNQYEVSCLELM
                     QRSGKAACDFLVYRWPKVKKISIFCGRGDNGGQGYVLAQQAKKMGMIPTVWQVGHQMS
                     MSKPPQMHKEVWYEMNSCHQQGIVLHTYSPDIDLGDPELIVDALFGVGLYGHVRPEIA
                     LLLQRLQQFTVPILAIEVPTGINASTGEIAGNALAATATITFLCMKLGLLINDGKIYS
                     GEIAFDDLLAPEEIYQQVKGIEESSLLDSSTCFSKKIWYRNKTQKGWQLSIN"
     gene            2045..3871
                     /gene="typA"
                     /locus_tag="ACF2SP_001831"
     CDS             2045..3871
                     /gene="typA"
                     /locus_tag="ACF2SP_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFD2359614.1"
                     /translation="MIEKIRNIAIIAHVDHGKTTLVDKLLQQTGTLNERAPKVERVMD
                     SNALEKERGITILAKNTCVYWKGYQINIVDTPGHADFGGEVERILSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFARGLNPIVVINKIDRPGARPDWVMDQVFDLFDNLGANDTQLDF
                     PVVYTSALNGYAKLNLEDNATDMSALLQTIVDKVTPPCVDANGPFQMQISSLDYSSYV
                     GTIGIGRITRGQIKAKSPVKIIDKDGNMRSGRLLQLLGFKGLERVEVEEASAGDIIAI
                     TGIESLNISDTICDPNLVEALPALLVDEPTISMTFQVNDSPLAGQEGKYVTSRKIRER
                     LNTELLHNVALRVEDCDDPDKFRVSGRGELHLSILIETMRREGYELAISKPEVIIREV
                     DGEQQEPYEHLTVDVEENHQGTIMEKLGERRGEMQNMVPDGKGRVRLDYIIPTRGLIG
                     FHNEFLSCTSGTGLMYHVFDHYGPLIKGRIGKRLNGVMIANCPGTARAFALFNLQERG
                     RLFLEPQSVCYEGMIVGIHARDNDLVVNVTKEKQLTNIRASGSDENIILTPPVKLSLE
                     QALEFIDDDELVEVTPLSIRLRKKFLKEHDRKKASRTATSEE"
     gene            3878..4765
                     /locus_tag="ACF2SP_001832"
     CDS             3878..4765
                     /locus_tag="ACF2SP_001832"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFD2359615.1"
                     /translation="MKQKFKRAILYARQHRANQEVNESLHRLVDFLSTQDIEIFQDSD
                     TAASFELKAPVLPREKMGAKHDLIIVVGGDGSLLSASRMAIKVNTPVIGINRGRLGFL
                     TDILPQDIESHLGPVLNGQYNEEERFLLHTKIYDKENSYFEGDALNDVVLGRGSETHL
                     IEFDVYINQQLVSHYRSDGMILSTPTGSTAYALSAGGPIMHPQLNAIVLVPMFSHSLS
                     SRPLVIDGEAEIELYISKSNETDLRISCDGHESRVVKPGQKVAVKKNGNRLRLLHPLD
                     YHYYDTLRSKLGWESKHQG"
     gene            4769..6436
                     /gene="recN"
                     /locus_tag="ACF2SP_001833"
     CDS             4769..6436
                     /gene="recN"
                     /locus_tag="ACF2SP_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFD2359616.1"
                     /translation="MLTTLRIEHFAIVKQLELDFTQGMSTFTGETGAGKSIMIDALLL
                     ALGEKADTSVVRPGEEKCDITAGFCFDESSEPAQWLAEHDISCHDGEIYLRRIIYAEG
                     RTKSYINGQPFPLQKIKEVSEKLVHIHGQHQHQTLMQHATHRQQLDRYANNHLLLNEV
                     TTLYKQYQQIVQEINALQSQEQQADRIKLLQFQIDELQTLGIREGEVEALHQEHQILH
                     HAREYLQLSQQINELLNSDDQPNICSGLNQIFQLIQQLPQEQAQIKNTAELINSALIQ
                     CEEAITEMQQFADKIQLDPERLQEVEARISSIHQLARKYHLDSKSLQEHEKTLQNELE
                     SLQNVESKLAHLHLQLAQINKAYESAALKLRESRKIHAKKLAQEITASIQQLGMPKGW
                     IEIEITPLEKMQAHGLDKVEYKVCTNPGMEPDSLNKIASGGELSRISLAIQMITAQKG
                     STPTLLFDEVDVGIGGSTAALVGKMLRKLGEKLQVFCVTHQPQVAASAHHHFLVTKHS
                     NNNQTFTHISLLKASDKIAEIARMMGGLTITEQTLSHAKELISLSNE"
     gene            6592..6798
                     /locus_tag="ACF2SP_001834"
     CDS             6592..6798
                     /locus_tag="ACF2SP_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD2359617.1"
                     /translation="MSEKIRGTVKWFNESKGFGFLESGGKDYFVHFSAIQGSGFKTLA
                     EGATVMFKACNGQKGPQAEEVEVV"
     gene            7001..8068
                     /gene="lpeA"
                     /locus_tag="ACF2SP_001835"
     CDS             7001..8068
                     /gene="lpeA"
                     /locus_tag="ACF2SP_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit LpeA"
                     /protein_id="HFD2359618.1"
                     /translation="MLGIQFNRGQWRSNYYWQIPVLIIAVISILFFISKKLFYSKVTP
                     ESIPNKLVEVEPIKSHNLQQTIHLLGTIHPKHATTLIAKESGMLDTLIPTGQKVTKGT
                     LIAKINNPDLEKNLQLSLSAVELAKAQYERITPLIKSGYVSTKEVEEKKQAWIDAQKE
                     LSKTRIELDNLRFYAPFDGIIGAYKKREGAQVNAGESVVSIYDPSALVVDFDIPCSNL
                     ATLNEGQPVYVLGKRYSLSHLQKMLDEDTHMCPADVDIQCDDCLIGATTSVELLVAEK
                     NNTIVIPFQAIFLRNSKPFVYLVKKGKIVLASVKTGLQQEDKIEIVEGLKAGQQLVTK
                     GQERLYPEMTVDIYHPATSSS"
     gene            8073..11117
                     /gene="lpeB"
                     /locus_tag="ACF2SP_001836"
     CDS             8073..11117
                     /gene="lpeB"
                     /locus_tag="ACF2SP_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit LpeB"
                     /protein_id="HFD2359619.1"
                     /translation="MKLTSYFIKHPVITIILNAMIVVLGLLCLYNLSVREYPDINFPT
                     ITVSASYPNASPDLVETAITNVLEDRLAGIEGLETITSQSSAGYAQITLMFRSGTSMD
                     RALSTTQDAVGVAKALLPPEVRSPTVERQRKSNGLPFIGISLESTSKDFGELTHYANL
                     NLKNVFRSVQGVASVEVWGQPYTYNITLQPEKLFSFGVNVDEIVNALAKNRISLPAGN
                     YRNKIPSTLNSELKTREDYENLLIKANSKHPILLKSLANVALETDNSQMRVRVNGHAG
                     VVLSINKANEANPIEVSKEIRKVIKGLQQGLPKDLKINTIIDQSDFINASIKNIRSAI
                     GEAIFLVLIIVFLFLRNIKATIIPLITIPISLLGSLLFLKLFGFSINLMTLLAMVLAI
                     GLVVDDAIIVLENIWRHIESGLSPLDAALKGAREIGFAIIAMTFTLSSVYLPIAFIQG
                     MLGQLFVEFAVALAGSVFISGIVALTLSPLMCANLLSASSKNWWPQFDNALEKLTKHY
                     SNILQFILKHQKITLLTALISVVACFGFYNLISHETAPKEDRGLIGIYIPPVAGEDIG
                     MLDNKTGKLEKKLDAIPEANNRLTFIGDWGGSIVLPLKPHAQRHRSANQIVEKLRPSF
                     NHFPSMDPHVWSWDTGLPGVDDAGSGSELTLVISTPDHFRQLFDETEKLKSVLDKSKL
                     FAAVNYDLRLDTMGYNIDLDYNQLAKLGLTANQVAKTIEVFFSGDKSQTFEKDGVVYN
                     ISIKGDSSPWTLNELYLTTNEGKSVSLGAITKMQPKAQPATLDHYQQMRSTTLHVQLH
                     KGDSMAKAIEKLWGTTKEVMPQHYKLTWIGAAKAFHESSNSMLFLFFLSLAFIYAILC
                     TQFENFTDPFIILFTVPLACSGALLFTYLFGQSLNIYTQVGLITLIGLISKHGILIVE
                     FANKLHKDGASLPEAIQKSAALRLRPILMTTGAMVFGAIPLVLSHDAGSESRHAIGTV
                     LISGLGLGTLFTLFVLPAVYNIIKSKLIDMQKTGGKKRCSKE"
     gene            11233..12117
                     /locus_tag="ACF2SP_001837"
     CDS             11233..12117
                     /locus_tag="ACF2SP_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HFD2359620.1"
                     /translation="MVPPEGFCFNYETSTSNSFQSFLSIKDIGNKALAEFTAMVLQLE
                     KEEIKVLTLSQSRDLPDAVFPNNWFSTHIDARGNNILIIYPLLAKNRQAEVNIKGLME
                     VLNRAQFEVQEIIDLRNDKDEILEGTGSLVLDRENRLLYASLSPRTSLNLVNKVADIL
                     GYTPIVFNSVDRQNKPVYHTNVIMGLGKCYAIICLDSINDAQQKAILSESFKETGKTM
                     IDISWNQVQHMCGNVLELYNKKGESLLILSEQAKRHFTHEQLRIIQQYSRLISTDIPT
                     IEAIGGGSARCMMAEIFY"
     gene            12264..13520
                     /locus_tag="ACF2SP_001838"
     CDS             12264..13520
                     /locus_tag="ACF2SP_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2828 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359621.1"
                     /translation="MKISELKKLVAELNKEVSSKVTCTNIINLAGNLSEIIEYFEQDE
                     HVGPELIYRIEAVTCEFWKLVSLTLPYEEWQSSIQVAPWLTLQQSLSKAGLLPTDFHH
                     PILYQSLKERYESFGHSELGIDQLLPLLIRCSRMTGYANKDPQSLDAYPHSPLNKQIE
                     ARRPQELAKLKDILCLLRAIFYLIHHCCTIEQLTLIPYLIYFRNPTTDEERRSELAIF
                     NWLIQKPADCLEFFKTNEDYIDTRSFRQINELAPLRPFIPTARSDFIKITNKEHWIYP
                     FIQSRTNTSRSEYDLLNDAVNWLDTDFATEKDKSYHAALEFAHTVKKQANILTQREMK
                     IVHSALYVFCLDKYIKHRKADPRPRCTPFSLSGETKCQAAEKKQQEILGKPTKFGFFE
                     NLALNEGRLKTLTKTFEMPPYTLLRN"
     gene            complement(13587..>14397)
                     /locus_tag="ACF2SP_001839"
     CDS             complement(13587..>14397)
                     /locus_tag="ACF2SP_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein SidH"
                     /protein_id="HFD2359622.1"
                     /translation="NELGPYGKVFLKQIMPDFIAKKSEIIKEIAIDNMETGLETQFKI
                     HAPAIFEKNKELKAAYEQLNVHLKEVQSLIEAEEKKPKGNPCREEKIAALRSHQSQLM
                     NTQRIPDHETLRFLQEQNKSAKSFMGKLEKYDTMISVYDSLTEIREHVSNHKSLSKEI
                     KDEKIQEISKMEDMLKTTSKEPSIRLAEVKAHGLSDQCKNVLLKNSDNFLVSFFKTLF
                     SKLFNIKNENETLVSSFKQRLQNIKGPEPVATPMETPENEAPLVNTNITRF"
BASE COUNT         4603 a         2809 c         2814 g         4171 t
ORIGIN      
        1 ccatgaaata tcgcaaattg ggaaaggtgg gacctgatgt ctcagcattg ggctttgggt
       61 gcatgggatt atcagagttt tatggcaaac ccgctcgcct tgatgatgca gtcaaaatta
      121 ttcgtaatgc gcatgaccaa taccaagtta atttctttga tacagctgat atttatggaa
      181 aaggtaaaaa tgaagaatta gttggtaaag cgataaaacc ctttcgtaac cagattatac
      241 ttgcaacgaa atgcggagta gtaagaacag gctctaatga tgaaatgagt gtcaataatt
      301 catctgctta tatcagaaca gcctgtgaga aaagccttaa acgcttaaat actgaccaca
      361 ttgatctcta ctatttgcat cgttatgacc atcaaacacc aattgaagaa gttatgggga
      421 taatgcatga ccttattgat gaaggtaaaa ttggctatat tggattatct gaaactgatg
      481 ctgataccat tcgggcagct tatacagtag tcaaggacaa actggttgct gttcaaactg
      541 aatattcact tcgagttcga gctccggcga cagcggtttt gggcacctgc cgtgaattgg
      601 gtatttgttt tgttgcctac tgccccatag cacgtggttt cctcagtgga aaaattaaga
      661 agcccaatct tttagggaca aatggctttg atttcagaac taatgttccg cagttccaac
      721 ctgaaaactt tgatcaaaat ctcggcttag tcaatgaact tgagaccata ggagaaaaaa
      781 caggttacac ccccgcacaa ttatcattag cttggctatt ggctcaagga gaagacatta
      841 tccccattcc cggaacctcg aatccacaac acttagcaga aaatatgcgc tcaattgata
      901 tttttctaac ccctgaacaa atgaaagatt tggaaaatgc ttacaaaaat aatcctgttg
      961 ccgggaaacg attgtccaag gaactgatgg cagcctttca tttaaaagaa taagctatat
     1021 gagaatagca taggagaatg acgctttctt aaccacaaat caggaaactc tccaacactc
     1081 taaaaaccat gtttacaaaa gttagaaaag atttttttat atggactatt attttactaa
     1141 gggaaaacca cagctggtca acatgaaaac gcctatttat cttgttacac aatttcaaca
     1201 actgatggac ttaatgcaaa accaatatga agtttcttgt ctcgaactta tgcagcgttc
     1261 aggcaaagca gcctgcgact ttttggtgta tcgttggccg aaagttaaaa aaatcagtat
     1321 tttttgtggt cgtggtgata acggaggaca aggctatgtt ttagctcagc aggcaaaaaa
     1381 aatgggaatg atcccaacag tttggcaagt aggacaccag atgagtatgt caaaaccgcc
     1441 tcaaatgcat aaagaagtat ggtacgaaat gaattcatgc catcagcaag gtattgttct
     1501 acatacttat tcgcctgaca ttgatttagg cgatcctgaa ttgattgtcg atgccctttt
     1561 tggcgtaggg ttgtatggtc atgttcgccc ggaaatagct ttgcttcttc aacgcctcca
     1621 acagttcact gtacccatcc tggctatcga agtgcctaca ggcatcaatg ccagcacagg
     1681 tgaaatagca ggtaatgcct tggctgcaac tgcgaccatt accttcttgt gtatgaaatt
     1741 aggcttatta ataaatgatg gaaaaattta tagtggagaa atagcattcg atgatttact
     1801 ggcacctgaa gaaatttatc aacaagtcaa aggcatagaa gagtcaagct tgcttgatag
     1861 ttctacttgc ttctccaaaa aaatttggta tcgcaataaa actcaaaaag gctggcagtt
     1921 gagcatcaat taaagagtag atattcttta atgagcgatt agtgtataat gttaaatcta
     1981 tgaacaattg tcagttatta ccttaaatta ttaaatctcg ctattcccca actaaaggtt
     2041 ttttatgatt gaaaaaattc gcaatattgc cattattgcc cacgttgacc atggcaaaac
     2101 tactcttgtt gacaagctct tgcaacaaac tggaacgctc aatgagagag ctccaaaagt
     2161 tgaacgcgtg atggattcca atgcacttga aaaggaacgt ggaattacta tcttagccaa
     2221 aaacacttgc gtttattgga aaggttatca aattaatatt gtagacaccc cagggcatgc
     2281 ggattttggc ggcgaagtgg aacgtattct ttctatggtt gacagcgttt tattgctggt
     2341 cgatgcagtt gacggcccca tgccacaaac ccgctttgtc acccagaaag catttgcccg
     2401 tggcttaaac cctatagtcg tgatcaataa aattgaccgt ccaggagcaa gacctgattg
     2461 ggtcatggat caggtgttcg acttgtttga taacttaggc gccaatgaca cccaactaga
     2521 ctttcctgta gtttatacct ccgctttaaa tggctatgcc aaacttaatt tggaagacaa
     2581 tgcaacagat atgtctgccc tgctgcaaac aatcgtagat aaagtcacac caccttgcgt
     2641 cgatgccaat ggtccgtttc aaatgcaaat cagctctctc gattattctt cttatgttgg
     2701 caccataggc attggccgga ttacacgtgg gcaaattaag gcaaaatctc ctgtaaaaat
     2761 tattgataaa gacggtaata tgcgcagcgg acgactgctc cagttactgg gattcaaagg
     2821 gctggaacgc gttgaagtcg aggaggccag tgccggtgat attatcgcga taaccggaat
     2881 tgaaagcctg aatatatccg acacgatttg cgaccccaac ctggtggagg cattgccagc
     2941 acttttagta gatgaaccta caatcagcat gacttttcag gttaatgact cgccgctggc
     3001 cggccaagaa ggtaaatacg tcacatcaag aaaaattcgt gaacgcttaa atacagaatt
     3061 actgcataat gtggcgcttc gcgtagagga ctgcgatgac cctgataaat tcagagtttc
     3121 tggtcgcgga gaattacact tgtcgattct tatcgaaacg atgcgccgtg aaggctatga
     3181 attggcaata agcaaaccgg aagtgattat tcgcgaggta gatggtgaac aacaggagcc
     3241 ttatgaacac ctgacagttg atgttgagga aaaccatcaa ggtaccatta tggaaaaatt
     3301 gggtgaacgc cgaggcgaaa tgcaaaatat ggttcctgat ggcaaaggca gagtacgttt
     3361 ggattacatc atcccgactc gcggattaat cggttttcac aatgaatttt tatcctgcac
     3421 ttcaggtacc ggattaatgt atcatgtctt tgatcattac ggcccgttga ttaaaggacg
     3481 catagggaag cgtctcaatg gcgtcatgat agctaattgc cctggtactg cccgggcttt
     3541 tgccttgttc aatttacaag aacgtggaag attattttta gagcctcaat cagtctgcta
     3601 tgaaggaatg attgtaggta tccatgccag agataacgac ctggttgtta atgtcaccaa
     3661 agaaaagcaa ttaacaaaca tcagggcatc aggttcggat gaaaatatca tcctgactcc
     3721 tccagtcaaa ttatctcttg agcaagcgct ggaatttatc gatgatgatg agttagttga
     3781 ggttaccccg ttatctattc gtttacgtaa aaaatttctc aaagaacacg atagaaaaaa
     3841 agcctctcgt accgccacaa gtgaagaatg acacattatg aaacaaaaat ttaaacgagc
     3901 catcttgtat gcccgtcaac acagagcgaa tcaggaagtc aatgaaagct tgcatcgact
     3961 ggttgacttt ttaagcacgc aagacataga aatctttcag gactcagata ctgcggccag
     4021 ttttgaatta aaggcccctg ttttacctag agaaaagatg ggggcaaaac acgacttgat
     4081 tattgtcgtg ggtggtgatg gcagtttact ttctgcctca cgcatggcta taaaagttaa
     4141 tacaccggtc attgggatta acagggggag gctaggtttt ttaacggata ttttacccca
     4201 agacattgag tctcatttag gccctgtgct taatggacaa tataatgaag aagaacgctt
     4261 cttattacat actaaaatat atgacaagga aaacagttat tttgaaggtg acgcactcaa
     4321 tgatgtcgta ttaggcagag gaagtgaaac gcatttgata gaatttgacg tttacatcaa
     4381 tcagcagctg gttagccatt atcgttcaga tggaatgatt ttatccactc caactggctc
     4441 aacagcctat gccttgtctg caggaggacc aataatgcat cctcaactca atgccattgt
     4501 cctggtaccc atgttttcac acagcctaag ttcccgacca ctcgttatcg atggggaagc
     4561 agaaattgaa ttgtatatca gcaaatcaaa tgaaaccgat ttgcgtatca gttgtgatgg
     4621 tcacgaatcc cgtgtcgtta aaccgggaca gaaagtagca gtcaaaaaaa atggtaatcg
     4681 attgcgtttg ttacaccctc ttgattatca ttattatgat acattacgaa gcaaacttgg
     4741 ttgggaatca aagcatcagg gataaaacat gctcacgaca ttgcgcattg aacattttgc
     4801 tattgttaaa caactggaac tcgattttac acaaggcatg tctaccttta caggcgaaac
     4861 aggcgctggc aaatccatca tgattgatgc cttactgtta gcattgggtg aaaaagctga
     4921 tacctccgta gtccgccccg gcgaagaaaa atgtgatatc acagccggat tctgtttcga
     4981 tgaatcatca gaacccgctc aatggctggc tgagcacgat atttcttgcc atgatggtga
     5041 aatctatcta aggcgcatta tttacgcaga aggccgcacc aaatcttata ttaatggcca
     5101 acctttccct ttacaaaaaa taaaagaggt aagtgaaaaa ctggtgcaca tccatgggca
     5161 acaccagcat caaaccctca tgcaacatgc gacacataga caacaacttg atcgctatgc
     5221 caataaccac ctcttgttaa atgaagtcac tacactctat aaacaatatc aacaaatagt
     5281 acaagaaata aatgcattac aaagtcagga gcaacaagca gaccgcatta aattattgca
     5341 atttcaaatt gatgaattac aaacactggg tattcgcgaa ggtgaagtcg aagccttaca
     5401 tcaagagcat caaattttgc atcatgccag agagtatctg cagttaagtc aacagatcaa
     5461 tgagctattg aatagtgatg atcaaccgaa tatttgctct ggtttaaacc agatttttca
     5521 actcatccag caactccctc aggaacaagc gcaaataaaa aacacagctg agttaattaa
     5581 ttcagccttg attcaatgtg aagaagcaat cactgaaatg cagcaattcg cagataaaat
     5641 tcagctggat ccggaaagat tgcaagaagt ggaagcccga atcagttcta ttcatcaact
     5701 agcccggaag taccatcttg atagcaaatc gcttcaggag catgaaaaaa cattacaaaa
     5761 tgaattggaa agtttgcaaa atgtagaaag caaattggca catcttcatt tgcaattggc
     5821 tcaaatcaat aaggcatatg aaagcgccgc attaaaatta agagaatcac gcaaaattca
     5881 tgcaaaaaaa ctggctcaag aaataaccgc cagcattcag cagttaggaa tgccaaaagg
     5941 atggattgaa attgaaatca ctcctttaga aaaaatgcaa gctcatggat tggataaagt
     6001 ggaatacaag gtatgtacta atcctggtat ggagcctgat tcattgaata aaattgcctc
     6061 aggcggagaa ttatcccgaa tcagccttgc aatacagatg attacagcac aaaaaggttc
     6121 aactcctacc ttattatttg atgaggtcga tgtgggtatt ggcggttcaa ctgctgcact
     6181 agttggaaaa atgctgagaa aacttggaga aaaattgcag gttttttgtg ttacccacca
     6241 gccacaagtt gctgcctcgg cacaccatca ctttttagtc actaaacata gtaacaacaa
     6301 tcaaactttt actcatattt cattacttaa agcatccgat aaaattgccg agatcgctcg
     6361 catgatgggt gggctcacta taacagaaca aacactgtct catgcgaaag agctgatatc
     6421 gctgagtaat gagtgaacac atcgatcgtt ttcaggtaaa attttagcaa tctattgatt
     6481 taatttccta ttttttatac aaaatataaa ataattcaaa aaattaaaaa aaaaacagca
     6541 caattaataa aataatgcta caatgtatgc gtaacaattt aggaaattaa catgtcagaa
     6601 aaaattcgtg gtacagtcaa gtggtttaat gagtccaaag gtttcggttt tttagaaagt
     6661 ggcggcaaag attattttgt gcattttagt gcgatccaag gcagtggttt taaaaccctg
     6721 gctgaaggcg ctactgtaat gtttaaagct tgcaatggtc aaaaaggacc tcaagctgag
     6781 gaagttgaag tagtctaatt tttttatatt agccctgttt ttttaagtat aatttaatcc
     6841 ttagaaaagc agggcttttt tttatctaaa acattcttaa aatttctgtt ctgcaattaa
     6901 aaaaactctg ctcaagcaac tgctttggca aatattggtt aaagccaact caattatatg
     6961 ataaagtttg tgccatcatt caattcaatt gcagaaaagc gtgttaggaa ttcaattcaa
     7021 tagaggtcaa tggcggtcaa actattattg gcaaatacct gtgctgataa tagctgttat
     7081 tagcattctg tttttcatca gtaaaaaatt attctactca aaagttacgc cagaaagcat
     7141 acctaataaa ctggtggaag ttgagcccat taaaagccac aacttacaac aaacaatcca
     7201 tttattaggt accattcatc caaaacacgc aacgacatta attgctaaag aatcaggtat
     7261 gttggacacc cttatcccaa ctggtcaaaa agtcacaaaa gggacactta ttgcaaaaat
     7321 caacaaccct gatcttgaaa aaaatctaca gctttcatta agtgcggtag aattggcaaa
     7381 agcacaatat gaacgtatca cccctttaat taaatcagga tatgtcagta caaaggaagt
     7441 cgaagaaaaa aaacaagcct ggattgatgc ccaaaaagaa ttatcaaaaa caaggattga
     7501 attagataac ctccgctttt acgctccttt cgacggaata attggagcat acaaaaagag
     7561 agaaggagca caagtcaatg caggtgaatc tgttgtaagt atttacgacc cttctgcttt
     7621 agttgtcgat tttgatattc cttgcagtaa tttggcaact cttaatgaag gtcagccggt
     7681 ttatgtgctg ggtaaacggt attcattaag tcatttacaa aaaatgctcg acgaagacac
     7741 ccacatgtgc cctgctgatg ttgatattca atgtgatgat tgtcttattg gtgcgaccac
     7801 aagtgttgag ctccttgttg cggaaaaaaa taacactatt gtcatcccct tccaggcaat
     7861 atttttgaga aatagtaaac cttttgtata tcttgtaaaa aagggaaaga ttgtgcttgc
     7921 ttcagtgaaa actgggttgc aacaagagga taaaattgaa attgtcgagg gattaaaagc
     7981 tgggcaacaa ctggttacaa aaggccagga acgcttatat cctgaaatga ccgttgatat
     8041 ttatcatcca gcgacaagta gcagctaata ttatgaaact caccagctat ttcatcaaac
     8101 atcctgtaat caccatcatc ttaaacgcca tgattgttgt tctggggtta ttatgtctct
     8161 ataatttatc cgttagggaa tatcctgata tcaattttcc tacaataact gtttcagcaa
     8221 gctaccccaa cgccagtccc gatttagttg aaaccgcaat aaccaatgtt ctggaggacc
     8281 gtcttgcagg tatagaaggg ttagaaacaa ttacgtcgca atcaagtgca ggctatgcgc
     8341 aaatcacctt gatgtttcgt tccggaacat ccatggatag agcactgagt accactcaag
     8401 acgccgtagg agtagccaaa gcacttctgc caccggaggt aagatcgcca acagttgaac
     8461 gccaacgcaa atccaatggc cttcctttta ttgggatttc attggaatca acatccaaag
     8521 actttggtga gctgactcat tacgctaatc ttaatttaaa aaatgtcttt cgcagtgtgc
     8581 aaggtgttgc ttccgttgaa gtatgggggc aaccctatac atacaacatt acccttcaac
     8641 ctgaaaaatt attttctttt ggagttaatg ttgatgaaat agtgaatgcg ctcgcaaaaa
     8701 atcgtatttc gttgcccgca ggtaattata gaaataaaat tcccagtact cttaattctg
     8761 agctgaaaac ccgggaagat tatgagaatc ttttaatcaa ggcaaattcc aaacatccca
     8821 ttttactcaa atcactggct aatgtcgcat tggaaaccga taactcacaa atgcgagttc
     8881 gcgttaatgg gcacgctggt gttgtgcttt ctatcaataa agcgaatgaa gccaatccta
     8941 ttgaagtctc taaagaaatt agaaaagtga ttaaaggatt acaacaaggg ttgcccaagg
     9001 atctgaaaat aaacactatt attgatcaat cggattttat taatgcctcc attaaaaaca
     9061 ttagatccgc gattggtgaa gccatttttt tagtcttaat catagtcttc ctttttttac
     9121 gtaatattaa agccaccatc atccctctaa ttaccattcc aatttcatta ttggggtcct
     9181 tgcttttcct taaattgttt ggtttttcaa tcaatttaat gaccttactg gctatggtat
     9241 tggcaatcgg tttggttgta gacgatgcga ttattgtctt ggaaaacatt tggcgtcata
     9301 ttgaaagcgg attatctcct ttggatgctg cgcttaaagg agcacgtgaa attggcttcg
     9361 ccattatagc gatgacattt acccttagca gtgtttattt gcctatagcc tttatccagg
     9421 ggatgctggg acagctcttc gttgaatttg ctgttgctct ggcaggcagt gttttcattt
     9481 cggggattgt tgccctgaca ctttcaccat tgatgtgtgc caaccttttg agtgcatctt
     9541 ctaaaaactg gtggccacaa ttcgataatg ctcttgaaaa gttaactaaa cattactcca
     9601 atattttaca attcattctg aaacaccaaa agattacttt gctaacagct ctaatttctg
     9661 tcgttgcttg ctttggattt tataatttaa ttagccatga aaccgctcct aaagaagatc
     9721 gaggtttaat aggtatttat atccctcctg tagcaggaga agacataggg atgttggaca
     9781 acaaaacagg aaaacttgaa aaaaaactag atgccatccc cgaggcaaat aatcgcctga
     9841 cttttattgg tgactggggc gggtcaatag tactgccatt aaaacctcat gctcaacgtc
     9901 atcgaagtgc gaaccagatt gttgagaaat taagaccttc ttttaaccac ttcccctcca
     9961 tggatcctca tgtatggagt tgggatacag gcttaccagg agttgatgat gccgggagtg
    10021 gatctgaatt gactctggtt atttctaccc cagaccattt caggcaatta tttgatgaaa
    10081 ctgaaaaatt aaaaagtgtt ctagacaaga gcaaattatt tgcagcagtg aactatgacc
    10141 ttcgccttga taccatggga tacaatatag accttgatta taaccaactg gcgaaacttg
    10201 gactgacagc aaatcaggtg gcaaagacga ttgaggtatt tttcagtggc gacaagtcac
    10261 aaacctttga aaaagatggg gtcgtttata atatatcgat taaaggagat tcctccccct
    10321 ggactttgaa tgaactttat ttaactacaa atgaagggaa gtctgtttca ttgggagcta
    10381 ttaccaaaat gcagcccaag gctcaaccgg caactcttga tcattatcag caaatgcgtt
    10441 cgaccactct ccatgtccaa ttacataagg gagactctat ggcaaaggct attgaaaaat
    10501 tatggggcac taccaaagaa gtgatgcctc aacactataa actgacctgg attggtgcgg
    10561 ccaaagcatt ccatgaatct tccaattcca tgttattttt gtttttctta tctttggctt
    10621 tcatctatgc cattttatgc acacaattcg agaattttac tgaccccttc attattctat
    10681 tcactgtacc actagcctgc tcgggcgcct tattattcac ttatttattt ggacaatcct
    10741 taaacattta tactcaggta ggtttgatta ctttaatcgg gctcattagc aaacacggta
    10801 ttttaatagt ggaatttgct aataaacttc ataaagatgg tgcttcgttg ccagaagcta
    10861 tacagaaatc agctgcattg cgtttacggc ctatattaat gacaactggc gccatggttt
    10921 ttggcgccat ccctttggta ttatcgcatg atgcgggttc tgaatcccgg catgccatag
    10981 gaactgtatt gattagcggg ctcggtcttg gaacactatt taccctattt gttttgcctg
    11041 cggtttacaa catcatcaaa tccaaactaa ttgatatgca aaaaacaggg ggcaaaaaac
    11101 ggtgcagcaa agaataaaga tttatttaaa atgcaataag tggatatgga tttttgcaaa
    11161 tactatcagt attctaggcg acatgatttc cccgctgact tatttatgac ttgtaaagga
    11221 tctacggtac taatggttcc accggaagga ttttgcttca attatgaaac atcaaccagc
    11281 aactctttcc aatcattcct ttctattaag gatataggta acaaggcatt agcagaattt
    11341 actgcgatgg tgttacaact ggaaaaagaa gagataaaag tattaaccct ctcacaaagc
    11401 cgggatttac ccgatgccgt ctttcccaat aactggtttt caactcatat tgatgcaaga
    11461 gggaataata ttctgattat ttatccatta ttagctaaaa acagacaagc tgaagtcaat
    11521 atcaaaggat taatggaagt actgaacaga gcccaatttg aagttcagga aataattgac
    11581 ttaagaaacg ataaggatga aatccttgaa ggaactggca gcctggtttt agatagagaa
    11641 aaccgccttc tttatgcgtc tttatccccg agaacttccc tgaatttggt aaacaaagtc
    11701 gctgatattc tgggatacac ccctattgta tttaatagtg tagataggca gaacaaaccc
    11761 gtctatcata ccaatgtcat aatgggccta ggcaagtgtt acgctattat ttgcctggac
    11821 tcaattaatg atgctcaaca aaaagcaata ctttcagaaa gtttcaaaga aacaggcaaa
    11881 acaatgattg acattagttg gaatcaagtg cagcacatgt gtggtaatgt tttggaactt
    11941 tataacaaaa aaggtgagag ccttttaatt ttatctgagc aagctaaaag acactttact
    12001 catgagcagc taagaatcat ccaacaatac tccagattaa tatcgacaga tatccccaca
    12061 atagaagcca ttggaggtgg aagcgcacga tgcatgatgg ctgagatttt ttattaaaag
    12121 ttcaaaaaat tttgctacca gtttaatttc ctttcagata ttctctagag cgagacttaa
    12181 gaaaaacccc aaggtcaagg taaaaaagat tggaggttta cttaaggcct gtaaaaaact
    12241 aatcattcag gcagggaatc atcatgaaaa ttagtgaatt aaaaaaactg gttgctgaat
    12301 taaataaaga ggtttcatca aaggttacat gcacaaacat aattaacctg gctggcaatt
    12361 tatcagaaat cattgagtat tttgagcagg atgaacatgt tggtccagag cttatatacc
    12421 gaatagaagc ggtgacttgt gaattctgga agctagttag tctcacatta ccttatgaag
    12481 aatggcaaag cagtattcaa gttgctccct ggcttacatt acagcaatca ttatccaaag
    12541 ccggattact gcctacggat ttccaccatc ctattttata ccagagctta aaagaacgct
    12601 atgaaagctt tggtcacagc gaattaggaa ttgaccagct cttacccttg cttatccgtt
    12661 gcagccgcat gactgggtat gccaataaag atccacaatc tcttgatgct tatcctcact
    12721 caccattaaa caaacaaatc gaagcgagaa ggccacagga acttgccaag ctaaaagaca
    12781 tcctctgcct tttaagagcc atcttctatt taatccatca ttgttgcacc attgagcaat
    12841 tgacgttaat tccttacctg atttattttc gtaatcccac tacagatgaa gaaagacgat
    12901 ctgaattagc catttttaac tggctaatcc aaaaaccagc tgattgcctt gaattcttta
    12961 aaaccaatga agattacatt gacacaagat catttaggca aattaatgaa ttggcaccat
    13021 tacgcccttt tatcccaact gcacgtagtg attttattaa aataactaat aaagaacact
    13081 ggatataccc ctttattcaa agcaggacaa atacatcaag atcagagtat gatttgttga
    13141 atgatgcagt caattggtta gacactgatt ttgcaactga gaaagataaa tcctatcatg
    13201 ctgctttgga gtttgcccat accgtcaaaa agcaagcaaa cattttaacc caaagggaaa
    13261 tgaaaatagt gcattccgcg ctctatgtct tttgtctgga taagtacata aaacatcgta
    13321 aagcagatcc tcgtccaagg tgcactccgt tttcactttc gggagaaaca aaatgccaag
    13381 cagccgaaaa aaaacagcag gagatactgg gaaaaccaac gaagtttgga tttttcgaaa
    13441 atctggcctt gaatgaagga agattaaaaa cactgaccaa gacttttgaa atgcctccct
    13501 atactttact aagaaattag ttaaatttaa ttgttgtttt gactgaaatg aaagattatg
    13561 tctgattaaa tcaaaactaa ttcgaattag aatctggtaa tattggtatt cactaaaggt
    13621 gcttcatttt ctggagtctc cataggcgta gcgacgggct ctggaccctt gatattttgc
    13681 agcctttgtt tgaatgatga aaccaaagtt tcattttcgt ttttaatatt aaataatttg
    13741 gaaaatagcg ttttaaagaa actcaccaga aaattgtcag aatttttaag taatacattt
    13801 ttacattgat cacttaggcc gtgagccttg acctctgcca aacgaataga aggttctttg
    13861 ctggttgttt ttagcatgtc ttccatcttt gatatttcct gaattttttc atctttgatt
    13921 tcttttgaca aactcttatg attactgaca tgctctctta tttctgtcag tgagtcatag
    13981 acactgatca tcgtgtcata tttttctaat ttacccataa aacttttcgc gcttttattc
    14041 tgctcctgca aaaacctgag tgtctcatga tccggaatac gttgggtatt cattaactga
    14101 gactgatggg atcttaatgc ggctattttt tcttcacggc agggattacc tttaggtttc
    14161 ttttcttctg cctcaatcag gctctgtact tcttttagat ggacgtttaa ttgctcgtaa
    14221 gctgctttta actccttatt cttttcaaaa atagctggag catgaatttt aaactgggtt
    14281 tcaagccctg tttccatatt atcaatggct atttctttga ttatttcaga ttttttggca
    14341 ataaaatcag gcatgatttg cttcaagaat acctttccat aaggaccgag ttcgttg
//
LOCUS       DAXLDT010000076        14367 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXLDT010000076 DAXLDT010000000
VERSION     DAXLDT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14367
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.076"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            130..3282
                     /gene="putA"
                     /locus_tag="ACF2SP_001840"
     CDS             130..3282
                     /gene="putA"
                     /locus_tag="ACF2SP_001840"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFD2359623.1"
                     /translation="MLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMM
                     AIKTSATKLVQSVRSERKKSTGIDSFLTEYALSSDEGIALMCLAEALLRVPDNATIDN
                     LIKDKLAGGDWGAHRGQSESFFVNATTWALMLTGKVLTPEKAENTLTKALLKLVNRSS
                     EAVVRKAVDKAMRIMSKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEAALTSADAA
                     RYFEAYKEAIISIGEKADKHSDVYRRPGISIKLSALHPRYSEFQYERVMAELPPKLLA
                     LSRLAKDYGIALTIDAEESERLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYV
                     LDWVAALARSKQRRIMVRLIKGAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAK
                     KILTMTDAIYPQFATHNAYSVAMILNLVGGYRDFEFQCLHGMGNELYEQIVPANCYGI
                     PCRIYAPVGSHEDLLPYLVRRLLENGANSSFVNRIVDDKAPISELVEDPVAKSRSLLD
                     KINKNIPLPEDIFLPVRKNSKGFDFTNRLERALLQQELAKIESKEWQASPMIAGRKLS
                     SDLLQTVMSPQQPAYAIGSVQQATLDDVEVALNQAKLAFESWSKKAVEERASCLNRFA
                     DLLQANMSELMVLTCREAGKTWSDGIAEVREAIDFCRYYAKKAQELMSSPQRFNGYTG
                     ELNELSLHPRGTILCISPWNFPLAIFTGQVVAGLVTGNCVIAKPAEQTPLIAAYAVKL
                     MHQAGIPEGVIQLIPGAGETIGAALVADKRIKAVLFTGSTDTANLINRTLATRGGEII
                     PLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQRCSALRVLYVQEEVYPRTVEL
                     LKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMRKHHEILYQCTVDDEALSG
                     YFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQINQTGYGLTLGIHSRIN
                     ETVDYIRQRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAGGPNYLIRLCHERTY
                     TVDTTAAGGNASLMSIPEEG"
     gene            complement(3476..4870)
                     /locus_tag="ACF2SP_001841"
     CDS             complement(3476..4870)
                     /locus_tag="ACF2SP_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2359624.1"
                     /translation="MNHSKKVLNVFSLVMINVIAVDSLRTLPISAKLGFSLVFYYIFA
                     ALTFFIPVALVAAELATAYPNTGGIYVWVREAFGRRAGFITIWLQWIYNVVWYPTMLA
                     FIAATLSYLIAPHLGNNKFYLLGTALTLFWVFTFLNCFGMKLSSIVSIIGASIGTLLP
                     MIVIIVLGAVWIFQDRPVAVNYPTTWLPDFSSLGNLSLFSAVLFGLIGMEMSAVHAEE
                     VKNPQRDYPKALFYSALLIISTLSLGSLAIVIVVPNDSLSVVSGLVDAYAIFFNSYNM
                     PWMTSVIAVLIILGGLSGVSAWIIGPTKGLLVSARDGSLPALFSRVNKYGSPVAILLT
                     QGVIFTVLSTVFILLDSINAAYWVLSDLSAQMALLVYIMMFAAAIKLRYSKPEQPRGY
                     TIPGGNLVMSLISGIGIICCIAAMIVGFIPPSQIPIKNVFLFECFLIGGLILFVFIPW
                     LFAKKHDEQLCSEE"
     gene            complement(5135..7810)
                     /gene="acnA"
                     /locus_tag="ACF2SP_001842"
     CDS             complement(5135..7810)
                     /gene="acnA"
                     /locus_tag="ACF2SP_001842"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFD2359625.1"
                     /translation="MKVGQDSLSTKSQLTVDGKTYNYYSLKEAEKKHFKGINRLPYSL
                     KVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDL
                     AAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNQERYE
                     FLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENNGQLYAYPDTLVGTDSHTT
                     MINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQM
                     LRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGR
                     DKHTIALVETYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSL
                     PVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLV
                     AKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRNAGLQTYLDQLGFNLVGYGCTTCIGNS
                     GPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCS
                     DLSREPIGQDKEGNDVYLKDIWPSNEEIAAEVAKVSGTMFRKEYAEVFKGDAHWQAIQ
                     TSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGS
                     IKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTR
                     YVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESF
                     ERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAMVPVTIERQDG
                     DVEKIETLCRIDTADELEYYKNGGILQYVLRKISS"
     gene            complement(7895..8521)
                     /locus_tag="ACF2SP_001843"
     CDS             complement(7895..8521)
                     /locus_tag="ACF2SP_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1689 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359626.1"
                     /translation="MYHYLFSCHKSQESIDGLIEQVKQLLNHVEMEQKAYFLNLLTAR
                     VAEFQNELKSETSNTINKQQILIQYERFAKTLLICIKQPERTSSAIHNYHKGSYYPVA
                     IHDKIKPDPTIENAAIATLGVSLALLLGSIPTFIFNPLFGVIMVSLAVTLLLPSGFYL
                     LIPDSPDTTSKKEEEKRIFMEGAKIINPDVRIEEFDEQPYLSASLIKT"
     gene            8705..9949
                     /locus_tag="ACF2SP_001844"
     CDS             8705..9949
                     /locus_tag="ACF2SP_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFD2359627.1"
                     /translation="MSDLKNLILNSSILLDTSSTVQNDLNPEQLINVHEDSDGGSDLL
                     TPESFILLMAEMLSTNNADTICEYEHQNSADNKVLQNLQFKEVEEDEQIEGLNEQKKS
                     LDNPLWHDVDLPVINEISGNQCEGQFESTLEKNVALTWINSENFQPPIVKPENQTDET
                     YKIWDSEITSIESGKLKLDFPKADYDGNNINPYQKEAINQPGNDSSKNHQLIKGNDSF
                     PILEDIKTNAEPGTKAIKSEVEATKVGTEIPGSSFQMNQKAGSDIQPPVVSNSHIPKN
                     FSIPIHVNHSQWSEQLSEHIVWLGHQDVKSALIKIHPEELGPLEINVKVVKDNASVNI
                     STHSVYVKEIVDQAIPRLREMMAQQGINLSEVHIGTDTSSRQGSQKNHHAEIDLIQSA
                     EDKNKITSLTRHLPKGLVDYFA"
     gene            9986..11188
                     /gene="mavA"
                     /locus_tag="ACF2SP_001845"
     CDS             9986..11188
                     /gene="mavA"
                     /locus_tag="ACF2SP_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavA"
                     /protein_id="HFD2359628.1"
                     /translation="MSHNYFRTCLTTKNNKFFWQKPLASERFDKLIADRKFQKAIAHS
                     RKNQLMYSFIESKIASDYALLIDTKLREQLKQLTLDDFNDISGFERKEKSNSRQQSYF
                     KLRQELEFFLKNDIQQHKSCPDAQLNAFRRWINISDLLLRHHCYEGFLLVFVNLQLIA
                     DKQLIDGLPVSVRNNYNQLCQLSSPIGNHGALRHFMNTHQGESDFTPLFFTYHAIGAL
                     DESLESLKDKEVLLKKQLKHLNKKISHLRTEATPGVIDVISELLKNQQQIPKKMMERR
                     GHLIQLMEEVRFTGKQLKQIQVNMRNQLEQRANLVGLIVKEQKATPRTLPEYFEKTYS
                     IIKYRFNRQSIATVKSSQLPESTASSNPSSSNLYKSKLLPHFWNRHGKSSSSYWEEVF
                     TPSCLNNK"
     gene            complement(11239..11556)
                     /locus_tag="ACF2SP_001846"
     CDS             complement(11239..11556)
                     /locus_tag="ACF2SP_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359629.1"
                     /translation="MKMVNAYVPRSFAIINLTFLLFITILFSNAASASNTNTDQYFSG
                     NTQLAYFIGIHSYPGNYYWRGPVYYKNAYWTKWRYIGYGCQKSCLIDRWSGRVIRCAK
                     RCF"
     gene            11732..12583
                     /locus_tag="ACF2SP_001847"
     CDS             11732..12583
                     /locus_tag="ACF2SP_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2359630.1"
                     /translation="MTMNTKVILITGCSSGIGFDAVFALKKRGHRVIASCRKTEDVQK
                     LLNKGVEALLLDVSNPISIQSAFAQLLIMTSGHLDVLINNAGYGQVGALEDISRDILR
                     QQFETNVFGLQDLTNLVIPVMREQGGGRIINISSILGVVSMPFRGAYNASKYALEGLS
                     DTLRLELSGSGINVITIEPGPINSRFRDNAVDLSLQQIPMEKSYFKNQYRNMLSNFKQ
                     KKADSFFTRDTDAVIKKLVHAVESRKPKPKYPVTFPAYFLIGLKRFFTTRLLDRFILF
                     LSRKELG"
     gene            13125..>14367
                     /gene="dsbD"
                     /locus_tag="ACF2SP_001848"
     CDS             13125..>14367
                     /gene="dsbD"
                     /locus_tag="ACF2SP_001848"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFD2359631.1"
                     /translation="MRKLIDSFMILLLIFFTQAAWSYTPGFESSNPDTVMHFITGNSA
                     FVYLSAFFGLGILLAFTPCVLPMVPILSGIIVGQNSISTGKAFKLSLSYVTGMAITYA
                     GAGMLAGYMGGTIQTIMQRPVVITLFSVVFVAMALSMFGFFELKLPTSLGNRLNKNNQ
                     LGKQRSYLSVGIMGVLSTLIVSPCVTAPLIGVLSYIGQNGQVIMGGLILFVMALGMGV
                     PLLIVGAGYGTLLPKAGSWMLKIKQLFGFIMLGIAIWMLSRILNQTITHILWAGLLMV
                     ASISLGALKSQNNWRGYLAQGLGILALISGGIIFYNTAIPSVKIKQASVENSPFIKIN
                     TLNALQLQLEQAKKVRKAVFLDFSADWCGDCQEMDTKVFNQPEIQKAMSDWVSIKVDI
                     SDKNAEVDSIKKAFSIYGTPTI"
BASE COUNT         4530 a         2577 c         2892 g         4368 t
ORIGIN      
        1 aaataagcca gtgtaagata tcataaccgg attttttcat tgttataata atgaatttaa
       61 tttttgtctt atatggtaaa aatttaataa atattttttt caattaccac tttagccttg
      121 gagaacatta tgctggaaaa gcagtccatt catttaccgg aaggattaag agctgctatt
      181 aataaagctt atcgcatgga tgagctttct ttaattacag agcttagtga acaggcagca
      241 cttgatcctc agcaaatgat ggcaattaaa accagtgcta caaaattggt tcaatcagtt
      301 cgtagtgaga gaaagaaaag caccggaata gattcttttt taacggagta tgccttatca
      361 agtgatgagg gaattgcctt aatgtgtctg gcagaagctt tattacgcgt tcctgataat
      421 gccaccatag ataacttgat taaagataaa ttagctggcg gtgattgggg ggcgcatcgt
      481 ggacagagtg aatcattttt tgtaaatgct actacttggg ctttaatgct aactggtaaa
      541 gttttaactc ctgaaaaagc tgaaaataca ttaaccaaag ctttactaaa attggttaat
      601 cgaagtagtg aggctgttgt tagaaaagcg gttgataaag ccatgcgaat tatgagcaaa
      661 caatttgtga tgggacgtac cattaatgaa gcgttagctc gagcgaaaaa aaaggaagac
      721 aggggataca gatattctta cgatatgctt ggtgaggctg ccttaacatc tgctgatgct
      781 gcaagatatt ttgaagccta caaggaagca atcatttcaa ttggagagaa agcggataaa
      841 cattcagatg tatatagacg ccctggtatt tccatcaaac tttctgcctt acatccacga
      901 tatagcgagt ttcagtatga gcgtgtcatg gcagaattac cccccaaatt attagccttg
      961 tctcgtttag caaaagatta tggtattgct ttgacaattg acgccgagga gtcagaacgt
     1021 ttagatcttt cgcttgatgt gatagagaaa gtgtttactg atgagagttt acagggatgg
     1081 aatgggttcg gtttggccgt tcaatcctat caaaaaagag ctttttatgt cttggattgg
     1141 gttgctgctt tagcaagaag taagcagcgg cgtattatgg tcagattgat taaaggagcc
     1201 tattgggata gtgaaatcaa aaaaacgcaa atgcaaggct tttctgaata cccggttttt
     1261 acccgtaaag tatttactga cgtgtcattt caggcttgcg caaaaaaaat cctgactatg
     1321 accgatgcca tttatccaca atttgccaca cataatgctt attctgtagc aatgattctt
     1381 aatttggtag gaggttatag agattttgaa tttcaatgtc tgcatggtat gggtaatgaa
     1441 ctttatgaac agattgttcc agcaaattgt tatggtattc catgccgtat ttatgcacca
     1501 gttggtagcc atgaggattt attaccgtat ttagttcgac gattactgga aaatggagca
     1561 aactcatctt ttgtcaatcg catagttgat gataaggcac ctattagcga gttagtggag
     1621 gacccggtag ctaaatcaag atcgttactt gataaaataa ataaaaatat accattgcca
     1681 gaggatatct ttttacccgt acgaaaaaat tctaaaggct ttgactttac aaatcgactt
     1741 gaacgcgcat tattgcagca ggaactggca aaaattgaat caaaagaatg gcaggcaagt
     1801 cctatgattg ctggtcgaaa attatccagt gacttactgc aaacagttat gtccccacag
     1861 cagccggcat atgctattgg ctcagtgcag caagccacac ttgatgatgt agaggtggca
     1921 ttaaatcaag caaaattggc ttttgaatcg tggagcaaga aagcggtaga agaacgcgct
     1981 tcttgtctaa accgatttgc agatttatta caggccaata tgtcagagct catggtattg
     2041 acttgtcggg aagctggtaa aacctggagt gatggcattg ctgaagtaag agaggctatt
     2101 gatttttgtc gatactatgc caagaaagct caagagctta tgagtagccc tcaacgcttt
     2161 aatggataca ctggggaact aaatgaattg agtttgcatc ctcgcgggac aatattgtgt
     2221 attagtccct ggaattttcc attggctata tttacgggac aagtcgtagc cgggctggtt
     2281 acaggcaatt gtgttattgc gaaaccggca gaacaaacgc cactaattgc agcctatgct
     2341 gtgaagttaa tgcatcaggc cggtatccct gaaggagtaa ttcaattaat tccaggcgct
     2401 ggagagacta ttggcgctgc tttggtcgca gataaacgaa ttaaagcagt attatttaca
     2461 ggctcgactg acacggcgaa tttgattaat cggacgctag ctactcgagg tggtgaaatc
     2521 attcctctaa ttgctgaaac aggtggtcaa aatgcaatga ttgtcgattc ttcagcatta
     2581 ttggaacaag tggttgttga tgctgtcact tctgcctttg gcagcgcggg gcagcgatgc
     2641 tcggcactta gggtattgta tgtgcaagag gaagtgtatc caagaactgt agagctatta
     2701 aaaggtgcca tggctgagct agtcgtaggg gatcctcaat ggttatcaac cgatgttggc
     2761 cctgttatag acaaggaagc attatctata ttgaaaaatc atgtcgaaaa catgagaaaa
     2821 catcatgaaa tattatatca atgcactgtt gacgatgaag ctttatcagg atatttcatg
     2881 ccgccaacag ctattgcaat tgatagtatc agcgctttgg aaaaagaagt atttgggcca
     2941 attttgcatg tgattcagtt taagagaaaa gatctggata aggtaattaa tcaaatcaat
     3001 caaactggct atggtttaac actgggaata cacagccgaa ttaatgaaac agttgactac
     3061 attaggcaaa gggttcacgc agggaactgt tatgtaaatc gtaatatgat tggcgcagta
     3121 gtaggtctac agccttttgg tggtgaaggg ttatctggca caggacccaa ggctggaggc
     3181 cctaactatt tgattcgact ttgccatgag cgtacctata cagttgatac aactgctgca
     3241 ggaggtaatg ccagtttaat gtctatcccg gaagagggtt agcagtatta gaaattcact
     3301 taagagaata ataaaatctt aagtgcattt attacaggag atctgcccct gtctggaacg
     3361 gcaatatttg gtcgatcttg tatgagggct gatctattac aattgattaa taactcttaa
     3421 gaatcatctt gaaaagccca ccaaaatttt ttgtgggctt acatttcttg ctcaattatt
     3481 cttcactaca taattgctcg tcgtgttttt tagcaaataa ccagggaatg aaaacaaata
     3541 gaataaggcc gccgatcaga aaacattcaa agagaaaaac attctttata gggatttgtg
     3601 aaggggggat aaatccgaca atcatagcag ctatgcagca gatgatccca attccagaga
     3661 taagagacat taccagattt ccaccaggaa tagtatagcc acgcggctgt tcgggcttgc
     3721 tgtatcgcaa tttaattgct gcagcgaaca tcatgatata taccaacaat gccatttgag
     3781 cacttaaatc gcttaatacc cagtacgcag cattaattga atcgagtaaa ataaagactg
     3841 tacttaatac agtaaatata acgccttgag tgagcagtat ggctaccggc gagccgtatt
     3901 tattgacacg ggaaaacagt gcaggtaagg aaccatcgcg tgcagacact agtaatcctt
     3961 tggttggacc tattatccag gctgaaacgc cactgaggcc tcctaaaata attaacacgg
     4021 caatcactga tgtcatccac ggcatattat aggaattaaa aaatatggcg taggcatcaa
     4081 caaggcctga cacaacactt aaactatcgt ttggtactac aataacaatt gccaaagaac
     4141 ctaatgataa ggtggatata attaaaagag ccgaataaaa aagtgctttt ggataatctc
     4201 tttgcgggtt ttttacttct tcagcatgaa cagcagacat ttccattcct ataaggccaa
     4261 ataaaaccgc tgaaaataaa gacagatttc ctaaagaact aaaatcaggt aaccatgttg
     4321 tggggtaatt gactgctaca ggtctatctt gaaatatcca aactgcgccc aggacaataa
     4381 tcactatcat aggaagtagt gtgcctatgg aggcgccgat aatacttaca atgctggata
     4441 gtttcatgcc aaagcaattc aaaaaagtaa acacccaaaa caaggtaagt gctgtgccta
     4501 ataaataaaa tttgttattc cccaaatgag gagcgattaa ataggataac gtggcggcga
     4561 taaaagctaa catagttgga taccaaacta cgttatagat ccattgcagc caaatagtga
     4621 taaatcctgc ccgtctcccg aatgcttccc tgacccagac ataaattcca ccggtgttag
     4681 gataggcggt agcgagctct gcagcaacca atgctactgg aataaaaaag gtaagggctg
     4741 caaaaatata ataaaagacc agagaaaatc caagttttgc gctaataggc aaggtcctca
     4801 aactatctac agcaatgacg ttgatcataa ctaaagaaaa aacgttcaga accttttttg
     4861 aatgattcat tatttatcct ttatgagtgc aataaacccg ggtattgaat ggggtataaa
     4921 tggtattatg ataagttaac agagtaaaga aaagtttatc tgtacagttt aattactgct
     4981 aaatatttat aagttgttgg gtttctcctg attatggtat caatttttac tgatattctg
     5041 atttcagctg cctagtgatg cagtgttcat gcgtctctag atattttgtg aacccaacac
     5101 tctaagggag aagggcacgc aatgtgccca attgttaaga gcttattttg cgcaatacat
     5161 actgaagtat tccgccattt ttatagtatt cgagctcgtc agcagtatca attctacaaa
     5221 gagtttctat cttctcaacg tccccatctt gcctttctat ggttacaggt accatcgcgc
     5281 ccggggttaa tttatcagag atttcaatac tgatgcgttc gctaccatcc aattttaatg
     5341 ttttacgagt agtaccctct ttaaattgca atggcaatat acccatgcca attaaattgg
     5401 aacgatggat tcgctcaaag ctttcagtaa tcactgcttt aacaccaagc aaattagtgc
     5461 cttttgctgc ccaatcgcga gaagagcctg ttccatattc tttaccagca atgataacca
     5521 agtcttgttg attttcctga taacgcatgg ctgcatcata aatcgacatg gtttctccag
     5581 taggaacata acgagtgacg ccaccttctt gtccaggagt catttcatta cgaatgcgta
     5641 tattggcaaa agtgcctcgc atcatgacct cgtggttacc gcggcgtgat ccataggagt
     5701 taaaatcttt ctcatctacc cctttggatt ttaaatataa gcctgctgga gagctggctt
     5761 ttattgaacc tgcaggtgaa atatgatccg ttgtaattga atcgccgaat agggctaaaa
     5821 cataagcctg cttgatgggt ttgagaggtt ctggtttcag acttaaattt tcaaaaaatg
     5881 gggggtgttg tatgtatgta gaatcaggat tccattcata agtttgtcct gagcttgttt
     5941 gaatggcttg ccaatgagca tctcctttaa atacttcagc atactcttta cggaacatgg
     6001 taccgcttac tttagcgact tcagcagcaa tctcttcatt agatggccag atatccttga
     6061 gataaacatc atttccttct ttgtcttgcc caatgggttc cctgcttaag tcgctgcaag
     6121 ttgtaccgca tagagcataa gcgactacca atgggggaga agccagccag ttggctctga
     6181 cttgcgggtg aacacgccct tcgaaatttc tattaccaga cagtacggat gaaacaacaa
     6241 gatcatgctc tgcaacacag tgggaaatat catcaggcaa aggacccgaa ttgcctatac
     6301 aagtagtaca gccgtaacca actaaattaa aacccagttg atccaggtag gtttgtaggc
     6361 cggcattcct taaatagtca gtaactactt tagaaccagg tgccaatgaa gacttaaccc
     6421 agggtttgcg ctgcaatcct ttctcaatcg ctttttttgc taccagtcct gctgccatca
     6481 gtacacttgg attagaggtg ttagtacagc tggttattgc cgcaatgact acatgaccat
     6541 gtttcatttg aaaatctttg tttttaacag caaaggtttt ttctttctct ttttctttac
     6601 caacttcaat taaaaaattg ttaaattcta caggtaatga actgagattg actttatctt
     6661 gggggcgttt ggggccagcc agagagggtt caacgctgcc taaatccaga tgtaaagagt
     6721 cagtaaacac aggttcttca ttgtctttgt cataccacat accttgtgct tttgcataag
     6781 tctcaacaag ggcaatggta tgcttatcac gaccagtcaa ttccaagtac tttattgttt
     6841 ccttgtctac cggaaagaag ccacatgttg cgccatactc gggagccata ttggaaattg
     6901 tcgctctgtc tgccaaaggt aaatcattaa gcccaggacc ataaaattca acaaatttac
     6961 caacaacccc tttttttcgc agcatttgag tgacggtaag gaccaaatca gttgcggtaa
     7021 tgccttcttt aagtttacct gataatttaa aaccgattac ttcggggatt agcatggata
     7081 caggctgccc caacatggct gcttctgctt ctataccacc gactccccag cccaggacac
     7141 ctagtccatt aatcatggtc gtgtgagaat ctgtgccgac caaagtgtcc ggataggcat
     7201 acaactggcc attattttca ctattccaaa cagttttccc taaatactcc agattgacct
     7261 gatgacaaat ccctgttccg ggtgggacga cctgaaagtt ggaaaaagct ttttgtcccc
     7321 agcgtaaaaa ctcatagcgt tcctgatttc tttctatctc tattttggta ttcacttcaa
     7381 gggcatccgc tgaagcaaat ttatctacca tgacggaatg gtctatgacc aaatcgacag
     7441 gagaaagagg agaaatttta tctgcgttac ctcccatttt tactatggca gtacgcatgg
     7501 cagccaaatc tactactgcg ggaacaccag tgaaatcttg catcaggact ctagttggtc
     7561 taaatgctat ttcatgctgg gatgttttat tatggagcca gtcggcaatt gccttgatgt
     7621 ctttggtcgt tactgtattg ccatcctcaa aacgtaataa attctcgaga agtactttca
     7681 aggagtaagg gaggcgatta attcctttaa aatgtttctt ttcagcttct ttcaaactat
     7741 agtaattata ggtttttcca tcaactgtta actgtgattt tgttgataga ctgtcttgac
     7801 ccactttcat aaagtactcc caaaatcatt attattaata atctctagga ataatagctc
     7861 aatttcagga agttttcatt cagtatcaat aaaatcatgt tttgattaat gatgcggaga
     7921 ggtaaggttg ttcatcaaat tcttcgattc taacatcagg attaatgatt tttgcgcctt
     7981 ccataaaaat ccttttttct tcttcttttt tgcttgttgt atctggagaa tctgggatta
     8041 gtagataaaa tccacttggt aatagcaatg tcactgccag tgataccatg ataacaccaa
     8101 agagtgggtt aaaaatgaat gttggtattg aacctaaaag caaagcaagg ctgactccaa
     8161 gagtagctat agctgcattt tctatagtgg gatcaggttt aattttatcg tgaatagcaa
     8221 caggataata agatccttta tggtaattat gaattgcaga ggaggttcgt tcaggttgct
     8281 ttatgcaaat gagtaaagtt ttcgcaaatc tttcatactg gataagtatc tgttgtttat
     8341 taatagtatt tgaggtctct gatttaagtt cgttttgaaa ttcggcaaca cgggcagtca
     8401 acaaatttaa aaaataagct ttttgctcca tttccacatg atttagtaat tgttttactt
     8461 gttcaatcaa gccatctata gattcctgag atttatggca gctgaataaa tagtgataca
     8521 tgataaattt ctctccctat aaaatatgtc aaaaaactag cattattttt ttaggggtta
     8581 tagtgagtat taggaggttt tacatgcatg caatattttt taactatccc ttgattattt
     8641 tttcagtaag cttgcagatg gtatgagttt tgctatatgt taatcataac acagggagtg
     8701 tgatatgtct gatctgaaaa accttatact caattcatcc atcttattgg atacctcttc
     8761 aacagttcaa aatgatctaa accctgagca attgattaat gttcatgaag attctgatgg
     8821 tggcagtgat ttattaactc ctgaatcttt tatactgcta atggccgaaa tgttatcaac
     8881 aaataatgca gatacaatat gcgaatatga gcatcaaaat tcagcagata ataaagtttt
     8941 gcaaaatttg caatttaaag aggtggaaga ggacgaacaa attgaaggac tcaatgagca
     9001 aaaaaaatcc ttagataatc cattgtggca tgatgttgat ttacctgtta ttaatgaaat
     9061 atcaggtaat caatgtgaag ggcagtttga atcaacacta gaaaaaaatg tggctttgac
     9121 atggattaat tctgaaaatt tccaaccacc aatagttaaa cctgaaaatc agacagatga
     9181 gacttacaaa atttgggact cggaaataac ttctattgaa tctggcaaac tgaaactgga
     9241 ttttccaaaa gcagattacg atgggaataa cattaaccct tatcaaaaag aggcaatcaa
     9301 tcaaccgggg aatgattcat cgaaaaatca tcaattaatt aaagggaatg actctttccc
     9361 gatattagaa gacattaaaa cgaatgctga gccaggaact aaagcaataa aatctgaagt
     9421 agaggcaact aaagtgggca ctgaaatacc tggtagttcc tttcaaatga accaaaaagc
     9481 cggttcagat attcaacccc cagttgtttc caactctcac atacctaaaa atttttcaat
     9541 tccaattcat gtcaatcatt cacaatggtc agagcaatta tctgaacata ttgtttggct
     9601 tggtcatcaa gatgtaaaaa gtgccttaat taaaattcat cctgaagaat tggggccttt
     9661 agaaattaat gtaaaagttg taaaggataa tgcctctgtt aacatatcta ctcacagtgt
     9721 ttatgtaaaa gaaattgttg atcaagcgat tcccagatta agagagatga tggctcagca
     9781 aggtataaac ttgtctgagg tgcatattgg aaccgataca agttcccgcc aaggttcgca
     9841 gaaaaaccat catgctgaaa tagatcttat ccaatcagca gaagataaaa ataaaatcac
     9901 ttcattaacc aggcatctac ccaaaggact ggtcgactac tttgcttagt ttaacaaatc
     9961 aattgttggt aaggagttta caaaagtgtc ccataattat tttcgtacct gtctgacgac
    10021 aaagaacaat aagttttttt ggcaaaaacc tttggcatca gaacggtttg acaagctgat
    10081 tgctgataga aaatttcaaa aagcaatagc tcacagcagg aaaaatcaat taatgtatag
    10141 ttttatagag tctaaaattg cctcagatta cgcactattg attgatacta aattaagaga
    10201 gcaattgaaa cagcttactc tggatgattt taatgatatt tcaggatttg aacgaaaaga
    10261 aaaatcgaat tccaggcaac aaagctattt caagttaagg caagagctgg agttttttct
    10321 taaaaatgac attcaacagc ataaatcttg ccctgacgct caattaaatg cgttcagacg
    10381 ctggataaat atatcggatt tactcttaag acatcattgt tatgaaggct ttcttttggt
    10441 atttgttaat ttgcaattaa ttgcagacaa acagttaatc gatggcttac ctgtaagcgt
    10501 aagaaataat tataatcaac tatgtcaatt aagttcacca attgggaatc atggcgcttt
    10561 gcgccatttt atgaatacgc atcagggaga gagtgatttt actcctttat tttttaccta
    10621 tcatgccatt ggagctttgg atgaatcgct tgaatcctta aaggacaagg aagttctttt
    10681 aaaaaagcaa ttaaaacatc tgaataaaaa aattagtcat ttaagaacag aagcaactcc
    10741 cggagtaatt gatgtcattt ctgagttatt gaaaaatcag caacaaatac caaaaaaaat
    10801 gatggagcgt agaggacatc tgatacagct gatggaagaa gttagattca ctggaaaaca
    10861 attaaaacaa atacaagtta acatgcgaaa ccaacttgaa caacgcgcta atttggtagg
    10921 attgatagtc aaagaacaaa aagcaacgcc aagaacgctc cctgagtatt tcgaaaaaac
    10981 ctatagtatt atcaagtaca gattcaatag gcagtctatt gctacagtca aaagctctca
    11041 gcttccagaa tcaacagcaa gcagcaatcc aagttcctca aatttgtata aaagtaaatt
    11101 gctcccccat ttttggaata gacatggaaa atcttcctcc agttactggg aagaggtatt
    11161 cacgccaagt tgtttgaaca ataaataaga gaattagatg aactctgctt catacaagcc
    11221 ctgtttatat gaatttggtt aaaaacaacg tttggcacag cgaatgactc gaccactcca
    11281 tcggtctatc aggcagcttt tctggcaacc gtagccaata tatctccatt ttgtccagta
    11341 agcgttttta taatatacag ggccgcgcca gtagtagtta ccagggtagc tatgaatccc
    11401 aatgaagtaa gcaagttgag tatttccgga aaaatattga tcagtattag tgttgctggc
    11461 acttgctgcg ttgctaaata atatagtaat gaacaataga aaagttaagt taataatagc
    11521 gaatgatctc gggacataag cgtttaccat tttcatgata aattcctttt atcgcaaatg
    11581 acattaaaat tatagctcaa taataaatca attggttcct atattattac tataaacatt
    11641 tttttaatta atattgctac caaatgagtt tttatagtgg tagtaattgg ataaagagct
    11701 tagtacgata gttaaaaatt aggcataggt catgacgatg aatacaaaag ttattttgat
    11761 cacaggatgc tccagtggga taggttttga tgctgttttt gctttgaaaa aaagaggaca
    11821 tagagtgatt gcatcctgca gaaaaacaga ggatgtccaa aaattactaa ataaaggagt
    11881 tgaggctctc ttgctggatg taagtaaccc tatttcaatc caaagtgcat tcgcgcagtt
    11941 gttaattatg acatcgggtc atctggatgt attgattaat aatgcaggat acggtcaggt
    12001 aggtgctctt gaggatattt ctcgggatat tcttcgccaa caatttgaaa cgaatgtatt
    12061 tggtttgcaa gatttgacca atttggttat tccagtcatg agggagcaag gtgggggacg
    12121 cataatcaat atcagctcta tacttggcgt agttagcatg ccatttcgag gtgcttataa
    12181 tgcctccaaa tatgcattgg agggcttaag tgacacactt cgactcgaat tgtctggttc
    12241 aggtattaat gtcataacaa tagagcctgg ccctataaat agtcgatttc gtgataatgc
    12301 agtggactta tctttacaac aaataccaat ggaaaaaagt tatttcaaga atcaatacag
    12361 aaatatgctg agcaatttta aacagaagaa ggcagactcg tttttcactc gggatactga
    12421 tgcagtgata aaaaaactgg ttcatgccgt ggagtcaagg aaaccaaaac ccaagtaccc
    12481 tgtaactttt ccagcttatt ttttaattgg tttaaaacga ttctttacta cccgtttatt
    12541 agataggttt attttatttt tatccaggaa ggagctaggt taaagcaact tattggtaat
    12601 attataaaat agtcgtaagt tggtgttgat agttagcaac tactgtacag acctctctta
    12661 aaaagatact atagttatta tgaaacttaa atacagagta attcattacc tgatactatg
    12721 cagcgtggtt ttgttaataa taaaaccagc agtgtttcat aacaataatc agcagactca
    12781 gagtaatacc caatatatac attctataac ggaatgccct cagaggacgg cattggtgaa
    12841 ttattgctcg tccagtgcct attatattat ttctgaagag gaattatctc tcggtaatgt
    12901 cttatccttg ttgacattta tcctttattt tttagttatt caatcaaaat acaaatcgcc
    12961 tgtatcccca atttttaaac caccgattgc ccactagagt aaatttgtca actttctcag
    13021 taggcttctt gccagccctg gctatgcaag aggtccagtg taggatactt gtactctggt
    13081 acaagtatcc accgttgtta tttattactt attgggacat caaaatgaga aaattaattg
    13141 attcgttcat gattttatta ctgatatttt ttactcaggc ggcgtggtca tatactcctg
    13201 gctttgaaag ttctaaccca gatactgtga tgcattttat tacaggaaat agtgcttttg
    13261 tttatttgag tgcttttttc ggattaggta ttttgttggc ttttacgccc tgtgttctac
    13321 cgatggttcc tattttatca ggaattattg taggacaaaa ttctatttct acaggaaaag
    13381 cctttaaatt atcactgagt tatgtgactg gaatggctat cacctatgca ggagcaggga
    13441 tgcttgccgg gtatatggga ggtacaatcc agactataat gcaacgtcca gtagtaatca
    13501 ctttattcag tgttgtattt gtagccatgg ctctttcaat gtttggcttt tttgaattga
    13561 agcttcctac aagccttggg aatcggttaa ataaaaacaa tcaattaggg aagcagagaa
    13621 gttatctttc tgttggaata atgggagttt tatccacttt aattgtttct ccttgtgtta
    13681 ccgctccttt aattggtgtt ttaagttata ttggtcaaaa tggccaggtc ataatgggcg
    13741 gattgatttt atttgttatg gcattgggga tgggggttcc tctgctgatt gttggtgcgg
    13801 gttatggtac tttactccca aaagcaggtt cctggatgct taaaataaaa caattgtttg
    13861 gttttatcat gttgggaatt gccatatgga tgttatcccg gattctgaat cagaccatta
    13921 cgcacatttt gtgggcagga ttactcatgg tcgccagcat cagtttaggt gctttaaaat
    13981 cacaaaacaa ttggcgtggt tatttggctc agggattggg tattcttgct ttgatttcag
    14041 gaggtattat tttttacaac accgcaatac cttcagtaaa aataaaacaa gcgtctgtag
    14101 aaaatagccc atttatcaag attaatacct taaacgctct tcaactgcaa ttggagcagg
    14161 ctaaaaaagt tagaaaagca gtatttcttg atttctcagc ggattggtgc ggtgactgtc
    14221 aggaaatgga taccaaagta ttcaatcaac cagaaattca aaaagctatg tctgattggg
    14281 ttagcattaa agtggatatc agtgataaga atgcagaggt tgattctata aaaaaagctt
    14341 ttagtattta tggaacgcca acgataa
//
LOCUS       DAXLDT010000077        13952 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXLDT010000077 DAXLDT010000000
VERSION     DAXLDT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.077"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            44..478
                     /gene="rplK"
                     /locus_tag="ACF2SP_001849"
     CDS             44..478
                     /gene="rplK"
                     /locus_tag="ACF2SP_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFD2359632.1"
                     /translation="MAKKVEAYIKLQIPAGKANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQQMEQGLPIPVVITVYSDRSFTFITKTPPASVLLKKAAGIQSGSGTPNTKKVAKLN
                     VSQLEEIAKVKKPDLTAADLAAAVRSIAGTARSMGIEVEGLE"
     gene            488..1183
                     /gene="rplA"
                     /locus_tag="ACF2SP_001850"
     CDS             488..1183
                     /gene="rplA"
                     /locus_tag="ACF2SP_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFD2359633.1"
                     /translation="MSKLTKKQKKIAEVIKPNQLYTVADAVAILKQFASKKFRESLDI
                     SINLGVDPRKSDQVVRSSTNLPKGTGKTVRVAVFAQGDNAAKATAAGADIVGFEDLAD
                     KIKAGEMNFDVVIATPDAMRIVGQLGQILGPRGLMPNPKVGTVTTNVEAAVNDAKSGQ
                     VRYRTDKNGIIHCTVGKADFAPEDVLENVVALINDLKKAKPASSKGQYLKKISLSTTM
                     GPGLPIDISSIPV"
     gene            1356..1889
                     /gene="rplJ"
                     /locus_tag="ACF2SP_001851"
     CDS             1356..1889
                     /gene="rplJ"
                     /locus_tag="ACF2SP_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFD2359634.1"
                     /translation="MTLNLAAKKAVVEEVTAVASKAISAVVADYRGLTVNQMTQLRSE
                     ARKSGVYLRVVRNTLTRRAFKNTEFECLNDLLVGPVFIALSLEAPSDAARLLKDYAKT
                     FEKLEIRALSVGGKVYNANQIDAVASLPTRDEAISKLMYVMKAPIEKFVRTLAEPHAK
                     LARTLAAVKDKKAGNPA"
     gene            1920..2300
                     /gene="rplL"
                     /locus_tag="ACF2SP_001852"
     CDS             1920..2300
                     /gene="rplL"
                     /locus_tag="ACF2SP_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFD2359635.1"
                     /translation="MAVSKNEILETISNMTVMEVVELIEAMEEKFNVSAAAAAVAVAA
                     PAAGAGAAAAEEQTEFTVVMTSFGSNKVNVIKAIRGITGLGLKEAKDLVEGAPSTVKE
                     GVSKDEAASIKKELEEAGATVEVK"
     gene            2392..6498
                     /gene="rpoB"
                     /locus_tag="ACF2SP_001853"
     CDS             2392..6498
                     /gene="rpoB"
                     /locus_tag="ACF2SP_001853"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFD2359636.1"
                     /translation="MAVAEAKPQYSHAEKKRFRKSFGKQTDIMPIPNLLEIQLKSYRD
                     FLQTDTKLSEQLNTGLHAAFSSVFPIESFSGNARLEYVGYKLGEPAFDVRECKLRGLT
                     YSAPLRVKIRLVVLDKDASDDPKPIKDIREQDVFMGEIPLMTDVGTFVVNGTERVVVS
                     QLHRSPGVIFEHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDCVYVRIDRRRKLP
                     VSILLRALGYEAEDILSEFFETTRCHLKNGEYHIDLIPQRLRGEIASFDIHVPETGEL
                     IVEQGRRITARHIKQMEKSQMQDLVVPRDYLIGKTLAKNIIDTSTGEFLAQANDEITE
                     ELLDAMANHGILQIDMIYTNDLDHGSYISDTLKIDPTGSQLEALVEIYRMMRPGEPPT
                     KEAAEALFKNLFFVEERYDLSAVGRMKFNRRVGIKSDEGPGTLTKEDILSVIKTLIDI
                     RNGIGMVDDIDHLGNRRVRSVGEMTENQFRVGLVRVERAVKERLSLVESENLMPQDLI
                     NAKPVSAAIKEFFGSSQLSQFMDQVNPLSGVTHKRRVSALGPGGLTRERAGFEVRDVH
                     TTHYGRVCPIETPEGPNIGLINSLSVYARTNEYGFIETPCRKVVNGRVTDEVEYLSAI
                     EEVDQYIAQSNVELDAQGNILADLVPCRHQNEFSLTTPDKINYMDVSPKQIVSVAASL
                     IPFLEHDDANRALMGSNMQRQAVPTLRSEKPLVGTGMERIVASDSGVSVVAKRGGVID
                     LVDASRIVVRVNDDETTAGETGVDIYNLTKYFRSNQDTCINQRPIVSTGDRIQRGDVL
                     ADGPCTDMGELALGQNLLVAFMPWNGYNFEDSILISERIVHDDRFTTIHIEELTCIAR
                     DTKLGTEEITADIPNVGESALSNLDESGVVYIGAEVKAGDILVGKVTPKGETQLTPEE
                     KLLRAIFGEKASDVKDSSLRVPSGMNGTVIDVQVFTRDGLEKDARAKSIEEEHLARVR
                     KDLIDERRIREEDIYHRVSHLLLDKVATGGPGSLKPGSKITQDYLDKVEREKWFDIRI
                     EDDAVSQQLEQLSKQLELLTKEMEKRFNDSRKKIIQGDDLAPGVLKIVKVYLAVKRRI
                     QPGDKMAGRHGNKGVISIVVPVEDMPHMEDGTAVDIVLNPLGVPSRMNIGQVLETHLG
                     LAAKGLGRKIAQMLDERQTPEAIKAYLEKIYNHDGVQRVNLKCLNDDELMTLADNLRA
                     GVPMATPVFDGATEQEIKSMLQLADLPADGKTVLIDGRTGNKFDNTVTVGYMYMLKLN
                     HLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVK
                     SDDVGGRTKIYKNIVDGDHRMDPGMPESFNVLLKEIRALGIDIELEHD"
     gene            6587..10792
                     /gene="rpoC"
                     /locus_tag="ACF2SP_001854"
     CDS             6587..10792
                     /gene="rpoC"
                     /locus_tag="ACF2SP_001854"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032829172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFD2359637.1"
                     /translation="MSDLLGILKQQGQSEEFDAIKIALASPELIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKTFGPVKDYECLCGKYKRLKHRGVICEKCGVELALAKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFEAFVVVDPGMTELERGQLL
                     NDEAYLDAMEQYGDEFDARMGAEAIRDLLRQIDLEDEIRNLREELPTTNSETKIKKIT
                     KRLKLLEAFYESGNKPEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQESVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLEFRGLAT
                     TIKAAKKMVEREESVVWDILDDVIREHPILLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAYNADFDGDQMAVHVPLTLEAQLEARSLMMSTNNILSPASGEPIIVPSQDVVL
                     GLYYLTREKVNALGEGKIYSSAQEAQNFYEAGHLDIHAKIKIRMPKEDGETGYHLVET
                     TVGRAILAEILPKGMPFDYINRTMTKKVISKVIDSCYRKFGLKETVIFADQLMYTGFK
                     YATRSGASIGIEDMEIPDDKSSIIEHADNEVREIESQFRSGLVTNGERYNKVIDIWSR
                     TNELVAKSMMSKIATEEVTDAKGNKVRQESFNPIFMMADSGARGSAAQIRQLAGMRGL
                     MAAPDGSIIETPITANFREGLNVFQYFISTHGARKGLADTALKTANSGYLTRRLVDVA
                     QDVVITEDDCGTDTGILMQPLIEGGDIVEPLHERVLGRVVASDVYIPTQTEPVVKAGT
                     LLDEEWVEKLEKHGVDQVMVRSPITCQTRFGLCAKCYGRDLARGHLVNTGEAVGIIAA
                     QSIGEPGTQLTMRTFHIGGAASRATAANNIQIKTKGVIRLHNIKTVTHENKNLVAVSR
                     SGEVTIVDEFGRERERYKVPYGAVISAQDNSPVEAGQVIATWDPHTHPVISEVSGRLK
                     FVDLIDGITMNRQTDELTGLSNIVIIDAKQRSAAGRDLRPMVKLVTDEGDDIYLAGTN
                     VPAQYYLPVDAIVNFEDGSLVGIGDVIARIPQERSKTRDITGGLPRVADLFEARKPKD
                     SAVMAEVSGLVNFGKETKGKRRLIINVSEDQCHEELIPKWRHISVFEGEHVERGEIIA
                     EGALNPHDILRLLGVGALANYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKRVITFA
                     GDSKFLVGEQVEESAMLQENDKLLAEGKQIARGTPILLGITKASLATESFISAASFQE
                     TTRVLTEAAVSGKVDELRGLKENVMVGRLIPAGTGYTYHQSRKAKRARAAAGGDSSAT
                     HTVTASDVEHALSEALNADNHEH"
     gene            10906..11286
                     /gene="rpsL"
                     /locus_tag="ACF2SP_001855"
     CDS             10906..11286
                     /gene="rpsL"
                     /locus_tag="ACF2SP_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFD2359638.1"
                     /translation="MATINQLVRKPRVDVKKKSNVPALESCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTSGVNDRKQGRSKYGTKKPKDKK"
     gene            11307..11834
                     /gene="rpsG"
                     /locus_tag="ACF2SP_001856"
     CDS             11307..11834
                     /gene="rpsG"
                     /locus_tag="ACF2SP_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFD2359639.1"
                     /translation="MPRRREVPKREILPDPKHHSELLAKFINVLMVSGKKSIAEKITY
                     GALSVMEERVKKIKKNEEDGSETGSSGSAGAVLRYFEEALDNVRPSVEVRSRRVGGAT
                     YQVPVEVRHDRSIALGMRWIVQAARTRGEKGMMLRLAGELMDAYENKGSAVKKREDTH
                     KMAKANQAFAHFRWN"
     gene            11849..13933
                     /gene="fusA"
                     /locus_tag="ACF2SP_001857"
     CDS             11849..13933
                     /gene="fusA"
                     /locus_tag="ACF2SP_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFD2359640.1"
                     /translation="MATPLKLYRNIGIAAHVDAGKTTTTERVLYYTGMSHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTCYWSGMDKQFESHRINIIDTPGHVDFMIEVERSLR
                     VLDGAVVVFDSVAGVEPQSETVWRQANKYGVPRIVFVNKMDRMGANFLRVVSQIKQRL
                     GSTPVVLQLPIGAEEEFKGVIDLIKMKAIHWDEENKGMTFKYVDIPADLKATCEEYRA
                     HIIEAAAEYSEELMEKYLEGEEFTEAEIKKALRHLTITNKVVPVFCGSAFKNKGVQAV
                     LDGVIEYLPSPTDIPDIQGVDEHGDEIHRKTSYDEPFSALAFKIATDPFVGTLTYFRA
                     YSGILKSGDTVYNSVKGKKERIGRLLQMHANSREEIKEVRAGDIAAAVGLKTVTTGDT
                     LCDQDKVVILERMDFPDPVIAVAVEPKTKADQEKMGIALGKLAQEDPSFRVHTDEESG
                     QTIIQGMGELHLEIIVDRMKREFNVEANVGKPQVAYRETLKQAVEQEGKFVRQSGGRG
                     QYGHVWLKIEPQEPGKGYEFINAIVGGVIPKEYIPAVDKGIQEQMQNGVIAGYPVVDV
                     KVTLFDGSFHEVDSSEMAFKIAGSQCFKQGALKAKPVLLEPIMSVEVVTPEDYMGDVM
                     GDLNRRRGLVQGMEDSPAGKIVRAEVPLAEMFGYSTDLRSATQGRATYTMEFCKYAEA
                     PTNIAEAIIKKQ"
BASE COUNT         4484 a         2434 c         3268 g         3766 t
ORIGIN      
        1 gctaattcaa taaaagctag cgcatgaccc ataaggagta ataatggcaa aaaaagtaga
       61 agcatatatt aaattacaga tccctgcagg aaaggcaaat cctagccctc cagttggacc
      121 tgctttaggt caacgtgggg taaatattat ggaattctgt aaagcattca atgctgcgac
      181 acagcaaatg gaacaaggtc tgcctattcc agttgttatc actgtatata gtgatcgtag
      241 ttttaccttt ataaccaaga cccctccagc ttcagttctg ctgaaaaagg ctgctggcat
      301 tcaaagcgga agtggcacac caaatactaa aaaagtggcc aaactgaatg ttagtcaact
      361 tgaagaaatt gctaaggtca agaagcctga tctgacagcg gctgatctag cagcggctgt
      421 aagaagtatt gcaggaacag cacgtagcat gggtattgaa gttgaaggtt tggaataagg
      481 ggttaccatg tcaaaattaa ctaagaaaca aaagaaaata gcggaagtta ttaagccaaa
      541 tcaactctat acagttgctg atgctgtagc tattctaaag caatttgcat caaaaaaatt
      601 tcgtgagagc cttgatatca gtattaattt gggagttgat cctcgtaaat cggatcaagt
      661 agtacgatct tctaccaatc tgccaaaagg tacaggaaaa acagtacgag ttgcggtttt
      721 tgcccagggt gataatgctg ctaaagctac tgctgcaggt gccgatatag taggatttga
      781 agatctggct gataaaatca aagcaggcga aatgaatttt gacgttgtta ttgcaactcc
      841 agatgctatg agaatcgtag gtcagctcgg acagattttg ggaccaaggg gactcatgcc
      901 caatcctaag gttggaacag taacaactaa cgtggaagca gcagttaatg atgctaaatc
      961 aggacaagta agatacagaa ctgataaaaa tggaattatt cattgtactg ttggaaaggc
     1021 agattttgct cctgaagatg ttcttgaaaa cgtagttgcg ttaatcaatg atctgaagaa
     1081 ggcaaagcca gcatcctcta aagggcaata tctgaagaaa atatctttat ctacaacaat
     1141 ggggcctggg ttacctattg atatttcgtc aatacctgtg taacatatca tcccattttt
     1201 aagaattcac ggcatagatt aggttccatg ccgtcgaaga ccgcaggtgc ctaggcttaa
     1261 tttcctgcgc agacggtgaa ccggctcctt aataaagaga cggccaccat aactgtcaag
     1321 tccatgagat ttgtacatat taggaggtca gtaacgtgac attaaattta gctgcaaaaa
     1381 aagccgttgt tgaagaagtg actgcagtcg cgtcaaaggc tatttcagca gtggttgctg
     1441 attatcgtgg gttaactgta aatcaaatga cgcaattgcg tagtgaagca cggaaatccg
     1501 gtgtttatct tcgtgtggtg cgaaatacac taacaagaag agcgtttaaa aacactgaat
     1561 ttgaatgctt gaatgactta cttgtaggtc cagtattcat tgcattgtca ctggaagcac
     1621 caagtgatgc tgcaaggcta ctgaaagatt acgcaaaaac atttgaaaaa cttgagatta
     1681 gagcattatc agttggcggg aaggtataca acgcaaatca aattgatgct gttgcgagct
     1741 tgccaacacg cgatgaggca atatccaagt taatgtatgt gatgaaagcg ccaattgaga
     1801 aatttgttcg tacacttgca gagccacatg ccaagttggc tagaactctt gctgctgtaa
     1861 aggacaaaaa agcaggcaat cccgcttaat cattatttaa ttttaattag gagctagtaa
     1921 tggctgtatc aaaaaacgaa attttagaaa caatttctaa catgacagta atggaagttg
     1981 ttgaattaat cgaggcaatg gaagaaaaat ttaatgtttc tgctgccgct gctgctgtag
     2041 ctgttgcagc gcctgctgca ggtgctggtg ccgctgctgc tgaagaacaa accgagttta
     2101 ctgttgtaat gaccagcttt ggttctaaca aggtaaacgt aatcaaggca attcgtggca
     2161 taactggtct gggcttgaaa gaagctaaag acttagtaga aggtgcacca tcaactgtta
     2221 aagaaggtgt atcaaaagat gaagctgcca gtatcaagaa agagcttgaa gaagctggtg
     2281 ctacagtaga agttaaataa ttaaaatata gcagtattta agaacccagc catgttcaca
     2341 tggctgggtt tactcgtttg aaaatcatca ataatttaca gaggaaccac catggcagtt
     2401 gcagaagcta aacctcaata ttcgcatgct gagaaaaaac gatttcgcaa aagctttggt
     2461 aagcagacag atataatgcc aataccgaat ctgcttgaaa ttcaacttaa atcctacagg
     2521 gattttcttc aaactgatac taaattaagc gaacaactga atacgggatt gcatgctgca
     2581 ttttcttctg tatttcctat cgaaagcttt tctggtaatg caagacttga atacgtcgga
     2641 tacaagcttg gagagcctgc atttgatgtt cgcgaatgca aactaagagg gctaacttac
     2701 tcagctcctc taagagtcaa gatcagactc gttgtgcttg ataaagatgc aagcgatgat
     2761 cccaagccta taaaggatat tagagagcaa gatgttttta tgggggaaat tcccctaatg
     2821 accgacgttg gtacgtttgt agtcaatgga actgaacgtg ttgttgtttc acaattacat
     2881 cgttcgcctg gagttatatt tgaacatgac aagggaaaaa ctcactcttc aggtaaatta
     2941 ctctattccg cacgaataat accttatcgt ggttcatggt tagattttga attcgatcct
     3001 aaagattgtg tttatgtacg tattgacaga cgtcgaaaac tgccagtgtc cattttgctc
     3061 agagcattgg gctacgaagc agaagatata ttaagcgagt tttttgaaac aactcgttgt
     3121 catctaaaaa atggcgaata ccatatcgat ttaattccac agcgtttgcg tggtgaaatc
     3181 gcctcattcg acattcatgt tcctgagaca ggagagctta tcgtagaaca aggtcgtcga
     3241 attactgcaa gacatataaa gcaaatggaa aaaagccaaa tgcaggatct tgtggtgcca
     3301 agagactatt taattggaaa aacattagca aaaaatatta ttgatacctc aaccggtgaa
     3361 tttctggctc aagcgaatga tgagataacg gaagagcttt tagatgccat ggctaaccat
     3421 ggtattttac aaattgatat gatctatact aatgatttgg atcatggttc ttacatatcg
     3481 gatacattaa aaattgatcc cacaggatca caactagaag cgctcgttga aatttaccgc
     3541 atgatgcgcc ctggagagcc tcctacaaaa gaagctgctg aggcactgtt caaaaatcta
     3601 ttctttgttg aagaaagata tgatctatct gctgttggca gaatgaaatt taatcgtcga
     3661 gtaggaatta agagtgacga aggcccaggc actttaacga aagaagacat tttatctgtc
     3721 attaaaacac tgatagatat tcgaaacggt atagggatgg ttgatgatat cgatcatcta
     3781 ggaaatcgta gggtaagaag tgttggtgaa atgaccgaaa atcagtttag agtaggtctt
     3841 gttcgtgttg aacgtgctgt taaagagcgc ttaagtttgg ttgaatctga aaatctgatg
     3901 ccacaggact taattaacgc aaaaccagta tccgccgcaa ttaaagagtt ttttggatcc
     3961 agtcagttat cacagtttat ggatcaagtg aatccattat ctggtgttac gcataaaaga
     4021 cgtgtatcag ctcttgggcc aggtggttta acacgtgagc gggctggatt tgaagtccgt
     4081 gacgtacata caactcacta tggtcgtgtg tgtccaattg aaacgcctga aggaccaaat
     4141 attggtctaa tcaattcact gtcagtttat gcaagaacta acgaatatgg atttattgaa
     4201 actccttgtc gaaaggtcgt taatgggcgc gtgacagatg aagttgaata cctatcggct
     4261 attgaggaag ttgaccaata cattgcacaa tccaatgttg agttagacgc acaaggaaat
     4321 attcttgctg acctagttcc ttgcagacat caaaatgaat tttcactgac taccccggat
     4381 aaaattaact acatggatgt ctcgcccaag caaattgtgt cagttgctgc ttcattgatt
     4441 ccgtttttag agcacgatga cgcaaacaga gcattaatgg gttcaaacat gcaacgacag
     4501 gctgtaccaa ccttgcgttc agaaaagccc ttggttggga ccggtatgga aagaatcgtt
     4561 gcatctgatt caggggtctc agttgttgcg aagagaggtg gagttattga tctggttgat
     4621 gcttctcgta ttgttgttcg agtgaatgac gacgaaacaa cagcagggga aacaggggta
     4681 gatatttata acttaaccaa gtatttccgt tctaaccagg atacttgtat caatcaaaga
     4741 cccatagtat caacaggtga tcgaattcaa cgaggtgatg tcctggctga tggtccatgt
     4801 acagatatgg gtgaattggc tcttggacag aatctactgg tagcgtttat gccttggaat
     4861 ggttataact ttgaggattc cattcttata tctgaacgaa tcgttcatga cgacagattc
     4921 actactattc acatcgaaga gttaacgtgt atagctcgag ataccaaatt aggtacagaa
     4981 gaaattacag cagatattcc caatgtaggc gagtctgcac tctccaattt ggatgaatct
     5041 ggagttgttt acatcggtgc agaagtaaaa gcaggcgata ttcttgtggg taaagtaact
     5101 cctaaaggcg aaacccaact aactccagaa gaaaaactgt taagagctat ctttggtgaa
     5161 aaagcttcag atgttaaaga ttcatctttg agagttccat caggcatgaa tggaacagtg
     5221 attgatgtac aagtcttcac ccgtgatgga cttgaaaagg atgcccgcgc taaaagcatt
     5281 gaagaagagc atttagcacg agttcgtaag gatttaatag acgaacgacg tattcgtgaa
     5341 gaagacatct atcatagagt ttctcatttg ctgttggata aggtggcaac aggcggtcct
     5401 ggaagcttaa agccaggatc taaaatcacc caggattact tagataaggt tgaaagagaa
     5461 aagtggtttg atattcgtat agaagatgac gcagttagtc agcaacttga acaattatcc
     5521 aagcagctgg aacttttaac aaaagaaatg gaaaagcgct ttaacgacag tagaaagaaa
     5581 attatccagg gagatgattt agctcctggt gtattaaaaa ttgttaaagt gtatttggct
     5641 gttaaaagaa gaattcagcc tggagacaaa atggcaggtc gtcatggaaa caagggagtg
     5701 atctccattg ttgttccagt tgaagatatg ccacatatgg aagatggaac ggctgtagat
     5761 atcgttctta atccattggg tgtaccatcg cgaatgaata tcggccaggt tcttgaaacg
     5821 catttaggac tagctgcaaa aggattagga agaaagattg cacagatgct tgatgaaagg
     5881 caaacacctg aagcaataaa agcatatctt gagaaaattt ataatcatga cggagtgcag
     5941 cgtgttaatt tgaaatgcct caatgatgat gaattgatga cactggctga taacctgaga
     6001 gctggtgtgc cgatggcaac tcccgtcttt gatggtgcta ctgagcaaga aatcaaaagt
     6061 atgttgcagt tagcagattt gccagcagat ggtaaaactg tgttgataga tggccgaaca
     6121 ggtaataaat ttgataatac ggttactgta ggctacatgt atatgttgaa gttaaaccat
     6181 ttggttgatg ataagatgca tgctcgttca actggatcat acagtttggt cactcaacaa
     6241 ccacttggtg gtaaagcaca gtttggtggt cagcgttttg gtgagatgga agtatgggca
     6301 ttagaagcat atggtgcagc atacacttta caagaaatgt taaccgtaaa atcagatgat
     6361 gtcggaggtc gaacaaagat ctataaaaat atagtcgatg gagatcatcg tatggaccca
     6421 ggaatgcctg aatcattcaa tgtattattg aaggaaatta gagcactggg aattgatatc
     6481 gagttggagc atgactaact catcagcgat acatactgac taaccatttt ttggaggcat
     6541 agacttggct accctaagca acgacccaat gaaaaaagca ccagtaatga gtgacttact
     6601 tggtattctt aagcaacaag gtcaaagtga agaatttgat gcaataaaaa ttgcattggc
     6661 gtcacctgaa ttaattcgat catggtccta cggcgaagtt aaaaagcctg aaacaataaa
     6721 ctacaggaca tttaagcctg aaagagatgg tttgttttgt gctaaaactt ttggtcctgt
     6781 taaagattat gaatgtttgt gtggaaagta caagcgacta aagcatcgtg gtgttatttg
     6841 cgagaagtgt ggggttgaat tggctcttgc caaagtgaga cgagaaagaa tggggcatat
     6901 cgaactcgct agcccagtcg ctcacatatg gttcttaaaa tctttaccat caagaattgg
     6961 tttgctcctc gatatgactc taagagatat cgagagagta ctctattttg aagcttttgt
     7021 cgttgttgat cctgggatga cagagcttga aagagggcaa ctacttaacg acgaagccta
     7081 tcttgatgcg atggaacagt atggtgatga gtttgacgcg cgtatgggcg cagaagcaat
     7141 tcgagattta ttgcgccaaa ttgatctcga agatgaaatt agaaatttac gtgaagaact
     7201 tccaacaact aactctgaga cgaagattaa aaaaatcacc aaaagactca agttattaga
     7261 agctttttac gagtcaggaa ataaaccaga atggatgatc atggatgtac ttccagtgct
     7321 tccacccgat ttgagaccat tggttccgtt agatggtgga cgttttgcta cttcagactt
     7381 gaatgattta tacagacgcg tcattaatag gaacaaccga ctaaaacgct tgctggatct
     7441 gaatgcgcct gacattatcg ttcgtaacga aaaacgaatg ctgcaagaat ccgtagatgc
     7501 tttacttgat aacggcagaa gaggtcgtgc catcactggt actaataaga gacctcttaa
     7561 atcattggca gatatgatta aaggtaaaca gggtcgtttc aggcaaaacc tcttaggtaa
     7621 gcgtgtcgac tactctggac gttcagttat tgtcgttgga ccaactttaa aattgcacca
     7681 atgcggattg cctaaaaaaa tggcgttgga gttattcaag ccatttatat tttccaaatt
     7741 ggaatttaga ggtttggcaa ctactattaa agcggccaag aaaatggtcg aaagagaaga
     7801 atcggtggtt tgggatatat tagatgatgt tattagagaa catcctatcc ttttaaaccg
     7861 tgctcctaca ttgcatagat tgggtattca agcatttgaa cctgttttaa ttgaaggtaa
     7921 agcaatccag ctccatcctt tggtatgtac agcatacaac gctgacttcg atggagacca
     7981 aatggctgtg cacgtgccct tgacactgga agcgcagctg gaagctcgtt cattgatgat
     8041 gtctacaaac aatatattat ctccagccag tggcgagcca attattgttc ccagtcagga
     8101 cgtcgtcctt gggttatact atttaacacg agaaaaggtt aatgcattag gcgaaggaaa
     8161 aatatattca agtgctcagg aagcgcaaaa tttctatgaa gcaggtcatt tggatatcca
     8221 tgcaaaaatt aaaattcgta tgcctaaaga agacggtgaa actggatatc atttagttga
     8281 gacaacagtc ggacgcgcta ttttagctga gattttgcct aaaggaatgc catttgacta
     8341 tataaaccgt acaatgacaa aaaaggttat ctcaaaagta atagacagct gttaccgcaa
     8401 attcggcttg aaagaaacag tcatttttgc tgatcagtta atgtataccg ggtttaaata
     8461 cgccacacga tctggagcat ccataggtat tgaagacatg gaaataccag atgataagtc
     8521 cagtattatt gaacacgccg ataatgaagt aagagaaatc gagtctcaat tcagatcggg
     8581 tttggtaacg aatggtgaac gatacaacaa agtaattgat atttggtcaa gaactaatga
     8641 attagttgcc aaatcaatga tgtctaaaat tgcaacagaa gaagtcactg atgcaaaagg
     8701 caacaaagta agacaagaat catttaaccc aattttcatg atggctgatt ctggagctag
     8761 aggttctgct gctcagatac gacagcttgc cgggatgcga ggtttgatgg cagctccaga
     8821 tggctcaatc attgaaacgc ctatcactgc aaacttccga gaaggtttga atgtattcca
     8881 gtattttatt tctacacacg gagctcgtaa gggtttggca gatacggcgt taaaaacagc
     8941 aaactcaggg tatctgacac ggcgtttggt agatgttgcg caggatgttg ttattaccga
     9001 agatgattgt ggtactgata caggtattct aatgcaacca ctgattgaag gtggtgatat
     9061 tgttgagcct ttgcatgaac gcgtccttgg gcgggtggtt gcttcagatg tctatattcc
     9121 tactcaaact gaaccagtag tcaaggctgg aactttactg gatgaagaat gggttgaaaa
     9181 attagagaag catggggtag accaggttat ggtcaggtca ccaatcactt gccaaacaag
     9241 atttggtctt tgtgcaaaat gctatggtag agatcttgct cgtgggcatc ttgtcaatac
     9301 aggcgaagca gttggtatta ttgcagcaca atcaatcggt gagccaggaa cacagttaac
     9361 catgcgtacc ttccacattg gaggtgctgc atcaagggca acagctgcga ataatattca
     9421 aattaaaacg aaaggggtta ttcggttaca taatataaag accgtaactc atgagaataa
     9481 aaacctggtg gctgtttctc gctcaggtga ggtaactatt gttgatgagt tcggacgaga
     9541 aagagagcga tataaagttc cttatggtgc agtaatttca gcacaggata attctcctgt
     9601 tgaagctggc caggttattg caacgtggga tcctcatacg catcctgtta tttccgaagt
     9661 gtcagggcgc ttgaagtttg ttgatcttat tgatggcatt acaatgaata ggcaaactga
     9721 tgaattaacc ggattaagca atattgtaat aattgatgcc aaacaacgga gtgcggcagg
     9781 tcgagatttg cgcccaatgg taaaattggt tacagatgaa ggggatgaca tatatcttgc
     9841 tggaaccaat gtcccggcac aatattatct acctgtcgat gccattgtga actttgaaga
     9901 tggaagtctg gtgggaatag gagacgttat tgcccgtatt ccgcaagaac gttctaaaac
     9961 tcgagatatt accggaggtt tgccaagagt agcggactta tttgaagcca gaaaaccaaa
    10021 ggattctgca gtaatggcag aagtatcagg attggttaat tttggtaaag aaaccaaagg
    10081 taagcgacgc ttgattatca atgtatcgga agatcaatgt catgaagagc ttattcctaa
    10141 atggcgtcat atttcagtgt ttgaaggtga gcacgtagag cgtggtgaaa tcatcgcaga
    10201 gggtgcactt aaccctcatg atatattgcg tttgttaggt gttggagcgc tggctaatta
    10261 cattgtaaat gaggtccagg acgtctatcg tttacaaggt gtgaaaatta atgacaaaca
    10321 cattgaagta attgtcagac aaatgctacg taagagagtt attactttcg ctggtgactc
    10381 aaaattcctc gtaggagagc aagtagaaga gagcgctatg cttcaggaaa atgataaact
    10441 tctagctgaa ggaaagcaga ttgcgcgagg tactcctatt ttactaggta tcacaaaagc
    10501 ttcattagct acagaatcgt ttatatcagc agcttcattc caagagacaa ccagagtctt
    10561 gactgaagca gcagtaagcg gaaaagttga tgaattgcgt ggtttaaaag aaaacgtgat
    10621 ggtaggtcgc ctaattccag caggaacagg ttatacttat catcaaagcc gaaaggcaaa
    10681 aagagcaagg gctgcagcag gcggagacag ttcagcaaca cacacagtta ctgcaagtga
    10741 tgttgaacac gcgttgagtg aagcattaaa cgcggataat catgaacatt aatcaggatt
    10801 caaaaatggc agatttaagc ttgacaaatc tgccgtacct atatagaatc cggcctcctt
    10861 atgcattcaa aatattcaca agtgacagat cggagttaag tacaaatggc tactattaat
    10921 cagttagtaa gaaagcctcg tgtggacgta aagaagaaaa gcaacgtgcc agcacttgag
    10981 tcatgtccac aaagacgcgg tgtatgtact cgtgtataca ctactacacc aaaaaaacct
    11041 aactcagcta tgcgtaaggt tgcacgtgtg cgcttgacca atggatttga agtaacatcc
    11101 tatattggtg gtgagggtca taatttacaa gagcactctg ttgttttaat tagaggtggt
    11161 cgtgttaagg atttacctgg tgtgcgctat catacagttc gaggtagctt agatacttca
    11221 ggagtgaacg accgaaaaca agggcgttcc aagtatggta caaaaaagcc aaaagataaa
    11281 aaataaccga ttgtaggaaa attgagatgc caagaagacg agaagtaccc aaacgagaaa
    11341 ttttacctga tccaaaacat catagtgaac tgcttgctaa gttcattaac gttttaatgg
    11401 ttagcggtaa aaaatcaatt gctgaaaaaa tcacttatgg tgcgctgtct gttatggaag
    11461 agcgtgtcaa aaaaattaag aaaaatgaag aagatggaag tgaaactggc agcagcggaa
    11521 gtgccggtgc cgtacttcgt tattttgaag aggctttaga taacgttcgc ccaagcgtgg
    11581 aagtacgttc acgtcgagtt ggtggtgcaa cataccaagt tccagtagaa gttcgacatg
    11641 atagaagcat agcgcttgga atgcggtgga tagttcaagc tgctcgcaca cgtggtgaaa
    11701 aaggcatgat gctgcgtttg gcaggggaac tgatggacgc ttatgaaaat aaagggtctg
    11761 cagtaaagaa acgtgaagat acacataaaa tggcaaaagc taaccaagcc tttgctcatt
    11821 ttagatggaa ttaagagaga gataagccgt ggcaactcca ttaaaactat atagaaacat
    11881 aggcattgca gcccacgttg atgctggaaa aacaacaact acagaacgag tattgtacta
    11941 taccggaatg tctcataaga ttggtgaagt acatgatggt gctgcaacta tggactggat
    12001 ggtccaggag caggagcgtg gtattaccat tacttctgct gcaacaacat gttattggtc
    12061 tggtatggac aaacaatttg aatcccatcg gatcaatata attgatacac caggacacgt
    12121 agactttatg attgaagtcg aacgctcttt acgtgtacta gatggagcag ttgttgtatt
    12181 tgattcagtt gccggcgttg aacctcaatc tgaaacagta tggcgtcaag ccaataagta
    12241 tggtgttcct cgtatcgttt ttgtaaataa aatggatagg atgggtgcca atttcctaag
    12301 agttgttagc cagattaagc aaagattggg ctctactcct gttgtgttgc aattaccaat
    12361 tggcgcagaa gaagagttca aaggtgtgat tgatctcatt aaaatgaagg caattcactg
    12421 ggatgaagaa aataaaggta tgacatttaa atatgttgat atccctgctg atttaaaggc
    12481 aacctgtgaa gaataccgtg cacacatcat tgaagctgcg gcagaatatt ccgaagagtt
    12541 aatggaaaaa tatttggaag gtgaggagtt cacagaggcg gaaattaaaa aggctcttcg
    12601 tcatctgaca atcacaaata aagttgttcc tgtattctgt gggtcagcct ttaagaataa
    12661 aggcgttcaa gcagtattgg atggcgtaat cgaatatttg ccatcaccca ctgacattcc
    12721 tgatattcag ggagttgatg agcatggtga tgaaatccat agaaaaacca gctatgatga
    12781 accattctca gcgttagcgt tcaaaattgc gactgaccca tttgtgggca cactaacata
    12841 ttttagagct tattctggta ttcttaagag tggcgataca gtctataact cagttaaggg
    12901 caaaaaggag cgtatcggcc gcttgttgca gatgcatgct aattctcgtg aagaaattaa
    12961 agaggtgcgt gcaggggata tagctgctgc tgttggtctt aaaacagtaa ccactggtga
    13021 taccctctgt gatcaagata aagttgttat attagaaaga atggattttc ccgatccagt
    13081 tattgcagtt gctgtagaac caaaaactaa agcagatcag gagaaaatgg gaattgcttt
    13141 aggcaagtta gcccaagaag acccttcttt cagagttcat actgatgaag agtcaggtca
    13201 gacaatcatt caaggtatgg gtgaattaca ccttgaaatt attgttgacc gcatgaagag
    13261 agagttcaat gtggaagcta atgttggtaa acctcaggtt gcataccgag agacactcaa
    13321 gcaagctgtt gaacaagagg gtaagttcgt aaggcaatca ggtggacgtg gtcaatatgg
    13381 acatgtatgg ttgaaaattg aaccacaaga accaggaaaa ggttatgaat ttatcaatgc
    13441 tatcgttggt ggcgtgattc ccaaagaata tatccctgca gtagataaag ggattcaaga
    13501 gcaaatgcaa aatggtgtaa ttgctggata tccagtggtt gatgttaaag ttacactgtt
    13561 tgatggttca ttccatgaag ttgattcaag cgaaatggca tttaaaattg ctgggtctca
    13621 atgcttcaag caaggtgcat taaaggcgaa accggtactt cttgagccaa ttatgagtgt
    13681 cgaagttgtt acacccgaag attacatggg cgatgttatg ggtgatctga acagacgccg
    13741 tggtttagtt caaggtatgg aagactcacc agcaggcaaa atagttagag cagaggtgcc
    13801 gcttgcagag atgtttggtt attcaactga cttacgttct gctacacaag gaagagcaac
    13861 ctacactatg gagttttgta agtacgcaga agcaccaaca aatattgctg aagcaattat
    13921 caagaaacaa taaatgttaa cgaggttatt ga
//
LOCUS       DAXLDT010000078        13874 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXLDT010000078 DAXLDT010000000
VERSION     DAXLDT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.078"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            104..1228
                     /gene="ceg10"
                     /locus_tag="ACF2SP_001858"
     CDS             104..1228
                     /gene="ceg10"
                     /locus_tag="ACF2SP_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg10"
                     /protein_id="HFD2359641.1"
                     /translation="MLTKSCAKATDQNRQELFMDSLLGDFQKMKEKALNQQQERRSKK
                     RELHCKSSEKTKKPNQYAALTHSQVQGVKAKARTVNDKFHLNAEEKKLWELILLGNQL
                     AQNISSCDLPTDNEDDASLVKLTQIFADETLERTDLTWLNKILKIALYSRGSGFGNCQ
                     EKAFFAFALLLHQAQKPESLIHSLRLATFNNHFILIVNERFLMDPWLNLAFPLSKGNQ
                     QLEIGYVFEGFGRLVNYFSINQEGQCFTHVVREGDTTERDLSSEKDMANCIHSLLDHR
                     DYFDLSIVSEREIHTKTKRRKYETQEQEVPQPRQETSNQPTETVYTDSNHNTWDNEGA
                     ADDFLEHFEQTHPVYNPGFFSFGQDDVGFKPDLPKNLFKF"
     gene            complement(1333..2544)
                     /locus_tag="ACF2SP_001859"
     CDS             complement(1333..2544)
                     /locus_tag="ACF2SP_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HFD2359642.1"
                     /translation="MFNQSQQKIVCVLYDDPKGGFPPNYARESIPELKQYPDGQSLPA
                     PDSIDFIPGEMLGSVSGELGLRQFLESNGHQLVVTSDKDGSDSVFARELKEATVVISQ
                     PFWPAYLTRDRIESAPKLKLAITAGIGSDHVDLQAAMEHNITVCEVTYCNSISVAEHT
                     VMMILALVRDFIPQYNTVIDGGWNIADCVSRSYDLEGMQVGCVAAGRIGLAVLRRLKP
                     FAVKLHYTDRHRLPLHLEQELNLTYHPSVESMVKVCDVVSIHCPLHPETEYLFDDRLI
                     KQMKRGSYLINTARGKICDQRAVADALESGHLAGYAGDVWFPQPPAKNHPWRSMPNHA
                     MTPHTSGTTLSAQARYAAGVREILECWLGNKPIREEYLIVSQGRLAGVGSHSYSAGNT
                     TTGTEQAVELV"
     gene            2752..3528
                     /locus_tag="ACF2SP_001860"
     CDS             2752..3528
                     /locus_tag="ACF2SP_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFD2359643.1"
                     /translation="MSEREWPANDYAIGSYIQSTVAEEYLAYLKANPTDKILDIGCGN
                     GAFTKNILLQVPQGSVLGIDASENMLHLAQDMSKKFPNFSVQKADVLTMTFNSQFDYV
                     VSFWCLQWACANIQKAFLNIINALKTGGKFLTLFPAGDDPFIMSYYALRKSGQFASLH
                     NFIPPVDYSHLNNLEKKLESLTCRDLNVKLCRQSITLPSLDVFRKFVNGIAFYQGQLP
                     NEEIQQINEAMVRYYDSECQTKYHGEYQFNFTIYLVTGEK"
     gene            3596..4780
                     /locus_tag="ACF2SP_001861"
     CDS             3596..4780
                     /locus_tag="ACF2SP_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2359644.1"
                     /translation="MHLKRTLSLTLLAFYGLGTILGAGIYALVGEVAKEAAQFTPLSF
                     LIASIIALFTAISYAELSSRLPHSAGSALYVRRAFHQKWLSGLIGWIVVLTGIISAAT
                     LSHGFVKYFQLFFPLPPSLIITILTVILAGVAIWGIRESATLILFMTLMEVGGLLMII
                     YYGRYSLASIDIKSFSFPHSFDGVLIGAFIAFYAFIGFEDMVNTAEETINPEKTLPKA
                     IFLALISATILYIAVAWVIVLTIPSNTLVKTDMPLIEIIKLQGQSPLLFSIIALISIT
                     NGILVQIIMASRLIYGMANQQNAPQIFSSVYSKTQTPVHSTLLVFLMILLFAYALPIT
                     TLAKLTSSIILCIFIIIHASLIKIKLTEKKPTNAISFPIVFPMISILLTLAFLIIQFF
                     LK"
     gene            complement(4791..6710)
                     /locus_tag="ACF2SP_001862"
     CDS             complement(4791..6710)
                     /locus_tag="ACF2SP_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359645.1"
                     /translation="MDNDQLSVIHYLWVGLPTKMNSSASIAGHDVAGPIKMAKALQSQ
                     AQGKPINPIKFWCLEQHQDFYQKLFNDAGVTIEVCGIEEIIRQESQGELRDQALFVQK
                     FLNDNLPSGQNSDIKQRVMFKDLFSLFLLVCQPGYFLDTNVFPATDREINLPGRDTVA
                     TAKSGFQKSNDFYLMYSPQRNDSQMSEIFDIWARNPSFGNLLCFSGSHVPYIEIEDLG
                     VQKISYKSYWGAKLPGLFFWLERNNRQLFEENLPYGDINQQLACSFSRKSLAPCSVCY
                     AEEAEPEKLLAMPFTTNEAYIATKANQIFYVNKTTKECVCVSDQFSSLFNCLPDRFHK
                     EKIRLASESEINQLIRSLDNFSHPSYIVNIADGTLLHHAVLSNNIKQVIMLLELGAKF
                     DLKASYQIKPEGTVLKFTPLELANYLKHEAIATLLQSHRISSTHVLSEISSESRIMPE
                     ATIQSSDEVIQPSSKDERGFTPEQIMVIKVAMQEALSSMGTSHFVSGRDDKKESLVQC
                     KSAFENAPNGTERHEALRNFVTAASTPRQGLLNFLPAAYGETRSAIAFFEHIKKQDSS
                     LFDALKPMREDKGPEVARQSNSSVETEATKPVARAREIMLHYKLAMQAERRGELKTDN
                     DSVDSQNLCGTRLKN"
     gene            6909..7790
                     /locus_tag="ACF2SP_001863"
     CDS             6909..7790
                     /locus_tag="ACF2SP_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2359646.1"
                     /translation="MDIRDIKSFLAVVEYHSLTLAAKKLHVTQSAMSKRIRKIEDELN
                     IRLIISEGSRLIFTEAAKQLIPYARQMLSAHQNMVHTLKNSQQFTQSIIIGASVYVSH
                     YVLPGFFKYLKETHPTIQAHLKTISEKEVEHILNHGTVDLVICPAREASEKLLSNSLL
                     WEEKLNLVVAVNHELAHRTSALSLSELSEYPALLTEKGAALRDKLDTLFEKNQLIINL
                     GFEMSTIDAIKSLVEYGLGWSYLPDSLLSEKLKILKIKDLELTIPFHAFYLKKRISDR
                     LINDLLCYFKKWQNRDN"
     gene            7963..9126
                     /gene="nosP"
                     /locus_tag="ACF2SP_001864"
     CDS             7963..9126
                     /gene="nosP"
                     /locus_tag="ACF2SP_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HFD2359647.1"
                     /translation="MKIESFQYIQNQGWSIPTFPALDSENTLILVFASPIFRANSKPI
                     EELAQAYKKSKIIGCSTAGEIFGPNIFDNSISVAVVQFESTKLQLAKTEVKSTEESYE
                     AGVHLSKSLHQKQNPQSIFVLSDGLNVNGSELIKGLNQHAGKGVTITGGLAGDGADFK
                     NTWIIYDGKIVDHCIGAVGFYGDQIHVGHGSRGGWDIFGPERVITQSKANILYELDNQ
                     PALKLYKTYLGDRASELPASGLLYPLAIRNPSAPDSQHIVRTILGVNEEEQSLIFAGD
                     LPKGYYAQLMRANFDRLIVSANEAGENANDRLFKNGSLNTQPVLAIDISCVGRRLVLG
                     ELTEEETETTLQSLPPKSMQIGFYSYGELSPLATGTCELHNQTMTITTLLESP"
     gene            9149..10564
                     /gene="nahK"
                     /locus_tag="ACF2SP_001865"
     CDS             9149..10564
                     /gene="nahK"
                     /locus_tag="ACF2SP_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosP-associated histidine kinase NahK"
                     /protein_id="HFD2359648.1"
                     /translation="MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYEND
                     QDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYWHYDADTDTATWSKGLFKILGLP
                     PSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIAR
                     ANPHKNEISGVLMDISKRKITEEQIHELNNKLVSTARLAGMSEVATSILHNVGNILNS
                     VNVSAIILKKNISKIHYKKLSAIKTMLDENINQLSEYLLNNPKGKLILPYLDALSKTL
                     HEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIHDVILNAVKSVILDK
                     HQIDVRIEIEPEIDEFITDKSKLFQILTNILANAKDSVLLHEANDSRTIVISIGRTEK
                     NHIMIKINDNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNG
                     NGKGAQFVLDLPINPKTLSTR"
     gene            10567..12792
                     /gene="narR"
                     /locus_tag="ACF2SP_001866"
     CDS             10567..12792
                     /gene="narR"
                     /locus_tag="ACF2SP_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosP-associated response regulator NarR"
                     /protein_id="HFD2359649.1"
                     /translation="MSTESFRILVIDDNPAIHQDFMKILNVSKNSVLLNKFDVELFDD
                     ETEQLGISLPEFEIDVATQGIEGIEKVKRSLEEGRPYPLAFVDIRMPPGIDGIETIKR
                     IWAIDPQIQIVICSAYSDYSWESTVNTLGLSDNLLVLKKPFDVIAVRQLASALTQKWV
                     LARETQKHTKFLNQLVEERTESLQQSFSLLRATIESSSDGILVVDLQGKIVDYNSQFV
                     KLWNIPESIIKKETSEPQLIQYMSTQLLNSEQHAQHVKEFSIHIDDSSMQIYHFRNGK
                     VVECCSKPHRVGLQTVGRVWSFRDITEQTRLKEKLEYQATHDALTNLPNRLLLIDRIE
                     HAISGYARHKMKFAILFFDLDRFKMINDSLSHEAGDQLLCDVAERLRSLVRKEDTLAR
                     LGGDEFVMLFQSFNSEEQIAGVAQKILKSFQKPFHIAERDINIHVSIGISVYPTDGKT
                     VNTLLSNADMAMYQAKFRGGNQFSFYTEKLNKKTNQQFQLELDLQKAIVHHEFFLLYQ
                     PQFTVNTHEIRSMEALIRWNHPQKGIILPLDFIPTAESSGLIVPIGEWVLRETCKQIG
                     AWRTMGLPDIRVACNVASKQLRQRDFPAMVEDLLKEYSVEPHCLELEITENVIIDKEI
                     QRTIKKLKSLGVLIILDDFGTGNSSLNLLKQVAVDSLKIDQSFIQNISKSPGDEAIID
                     AIIAIAQSMNFNIIAEGVETQNQLKFLKKRRCNDIQGFLMSKPIPPEEIEKLLQGNRT
                     K"
     gene            complement(12858..>13874)
                     /locus_tag="ACF2SP_001867"
     CDS             complement(12858..>13874)
                     /locus_tag="ACF2SP_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ras GEF/LegG2"
                     /protein_id="HFD2359650.1"
                     /translation="IANNLPNSIDRKQLSSESRLIFKDCEEKFKRLFNLSTTYNEHTN
                     LKRQFKTALIPALPSLLTVQIYTKDAYDNSVSDFERIQEKLRQLDIEHNRFLNQLQND
                     HSTWSKAYYDYMSNVYLPQSIFEYTTLLREYEELITTHGGPIAKQFHEDKNSEHLVSF
                     VESTLLENQSDLKLSPFQNERIVEMLSEIDVVKSECSSPDVFDDISWKLREMNCTLVK
                     AKSIGDYTSKIFANLDTPYMEENTDEDPKPVLVEELQTDDVYQLHHSGMKLIRELEQA
                     IEDHKPKVIYHCSGEANYGFFKEARRRGRHLSPEPMDIEVMDVDVMDYDENDLRNQTV
                     MITV"
BASE COUNT         4409 a         2789 c         2564 g         4112 t
ORIGIN      
        1 gcagtctcag tgaaccaggc tctgttttta caacagtcca gctcattatc atcagaggtt
       61 caacttgtca tatcgtaatt aactattata aagagagtgt aacatgctca caaaatcctg
      121 tgccaaagcg actgatcaaa atagacagga attatttatg gacagccttt taggtgattt
      181 tcaaaaaatg aaagaaaaag cacttaatca gcaacaagaa agacgctcca aaaaaagaga
      241 acttcactgc aaatcttctg aaaaaacaaa aaagccgaat cagtatgccg cccttactca
      301 ttctcaagtt cagggagtta aagcaaaagc ccgaacagtt aatgataaat tccatctcaa
      361 tgctgaagaa aaaaaactct gggagctgat tctgcttggc aatcaattgg cacaaaacat
      421 cagttcctgt gatttaccca ccgataatga agacgatgct tcactggtta agctaactca
      481 aattttcgct gatgaaactc tggaaagaac tgatttgacc tggctaaata aaattctgaa
      541 gattgctctg tatagcagag gttcaggttt tggtaattgc caggaaaaag ctttttttgc
      601 ttttgcactt ctgttacacc aggcacaaaa accagaaagc ttaattcact cccttcgatt
      661 agcgaccttt aataaccatt ttatattaat agtcaatgaa cggtttttaa tggatccctg
      721 gttgaatttg gccttcccct tatcaaaggg taatcaacaa ctggaaatag gttacgtttt
      781 tgagggattc ggccgacttg tcaattattt ttcaatcaat caggaaggtc aatgttttac
      841 ccatgtagtg cgagaaggag acactacaga gcgtgaccta tcatctgaaa aagacatggc
      901 aaactgtatt cattcgctac ttgatcacag ggattatttt gatctatcaa tcgtttcgga
      961 gagagaaatt cataccaaaa caaaaagaag aaaatatgag actcaggaac aagaagtgcc
     1021 tcaacctaga caagaaacat ccaatcagcc tacagaaaca gtatatacag actcaaacca
     1081 taacacttgg gataacgaag gagccgcaga cgattttcta gagcattttg agcaaacaca
     1141 ccctgtctac aatccaggtt tcttttcatt tggccaagac gatgttggtt tcaaaccaga
     1201 ccttccaaag aatttattta aattttaaat catccactgt ttgccccttt ccctgaactg
     1261 ccgctgtctt taaaggcagc ggctactttg aatgaaaaca gaaagacata agacagcata
     1321 agcgacgggg aatcaaacca attctactgc ttgctcagtt cctgtagtag tattacccgc
     1381 actataagaa tgagaaccaa ccccggctaa gcggccttga gacacgatca gatattcctc
     1441 cctaattggt ttattcccta accaacactc taatatttct ctgactcccg ccgcatatcg
     1501 cgcttgcgcg gacagggtag ttcctgatgt atgtggtgtc atggcgtgat ttggcatcga
     1561 tcgccaagga tggtttttgg ctgggggttg tggaaaccaa acgtccccgg cataaccagc
     1621 caaatgccca gattccagag catccgctac agcacgctga tcgcatattt ttccacgggc
     1681 ggtattaatt aaataagagc ctcgtttcat ctgttttatt aatcgatcat caaaaagata
     1741 ttctgtttcc ggatgcaatg gacaatgaat cgatacgaca tcacaaactt tcaccatgga
     1801 ttcgacagaa gggtgatagg tcaaattcag ttcctgttcc aagtgtagtg gtagtctatg
     1861 tctgtctgtg taatgcagtt ttacagcaaa tggctttaaa cgtcttaaaa cagccaatcc
     1921 aattcgccct gcagcaacac atcctacctg catgccttcc aaatcataag agcgactgac
     1981 gcaatccgca atattccacc cgccatcaat taccgtgttg tattgtggaa taaaatcccg
     2041 caccaaggct aaaatcatca tcacggtatg ctcagcaaca ctgatgctgt tacaataagt
     2101 gacttcacaa acggtgatat tatgttccat ggccgcttgc aaatcgacat gatccgagcc
     2161 gatacctgcg gtgatagcga gcttaagctt aggtgcactt tcaatacgat cacgagttaa
     2221 ataagcaggc caaaaaggtt gggaaatgac gacagtagct tcttttagct ccctggcaaa
     2281 tactgaatca gagccatctt tatcggaggt tactaccaat tgatgcccat tgctttcaag
     2341 aaattgtctc agtcccaatt caccactcac agaacctagc atttcacctg gaataaaatc
     2401 aatcgaatca ggagcaggta aactttggcc atcaggatat tgtttcaatt ctggaatgga
     2461 ttctcttgca tagtttggag gaaatccccc cttaggatca tcataaagca cacaaacaat
     2521 tttttgctga ctctggttga acatacctgg ctccttttca taatttgaag taaaaacatc
     2581 ctgatttttt tacagcattc actatacttg atttaagtca ttcctctcaa taaaaatact
     2641 attcataaaa gaattcatga tctattttag gggctgtcca tgatgtatca tgaataatga
     2701 tgacctgaac aatcatggaa taaataatca tctcaaaaga aaggaataag tatgtcagaa
     2761 cgtgagtggc cagccaatga ctatgccatt ggatcttata ttcaatcgac tgtggctgaa
     2821 gaatatttag catacctgaa agcaaatccc actgataaaa tattggatat tggatgcggt
     2881 aacggcgctt ttacaaaaaa cattcttctg caagtgcctc aaggctctgt attaggcata
     2941 gacgcttcag agaatatgtt acatttagcc caagacatga gtaaaaaatt ccctaatttt
     3001 tcagtccaaa aagcggatgt actcactatg acattcaact cacagtttga ttatgtcgtc
     3061 tctttttggt gcttgcaatg ggcatgtgcc aatattcaaa aagcattttt aaatatcatt
     3121 aacgcattga aaacaggggg aaaatttctt actctttttc cagcaggaga cgacccgttt
     3181 atcatgagtt attatgcttt aagaaagtca ggacaatttg catccctgca taattttatc
     3241 ccccccgtgg attactctca cttaaacaac ctcgaaaaaa aactggaatc acttacctgc
     3301 cgcgatctta atgtgaaact atgccgacaa tccattaccc tgccatccct ggatgttttc
     3361 aggaaatttg tcaatggcat tgccttttat caagggcaat tgcccaatga agagatacag
     3421 caaatcaatg aagcgatggt tcgttattac gatagcgaat gccaaacaaa atatcatggt
     3481 gaatatcaat tcaatttcac tatctatctg gtaaccggtg aaaaataaaa gaggtaacaa
     3541 ataaaaaaga aaatccgttc tacgactata gatctccaga taaaatgggg gagcgatgca
     3601 cttaaaacga actttatcat taacactgct agccttttat gggcttggaa ctatattggg
     3661 cgcaggaatt tatgcccttg ttggtgaggt cgcaaaagag gcggcacaat ttaccccact
     3721 atcattctta attgcgtcca tcatcgcgct ttttactgcc atttcttacg cagaattaag
     3781 ctcaaggtta ccgcatagcg caggctctgc tctttacgtg cgtagggcat tccatcaaaa
     3841 atggctatct ggcttaatag gctggatcgt ggtcctaaca ggtatcattt cagcagccac
     3901 cctatcccat ggttttgtca aatattttca attgttcttt ccattaccac cttctctcat
     3961 catcactata ttaactgtca ttttagcggg ggttgctatt tggggtattc gtgaatcagc
     4021 gactcttatc ctgttcatga cgctcatgga agtgggtgga ttattgatga ttatttatta
     4081 cggtcgttat tcattagcct ctatcgacat caaatccttc tcttttcctc attcttttga
     4141 tggagtgctt attggagctt ttattgcgtt ttatgctttt attggttttg aagatatggt
     4201 taataccgcc gaagaaacaa taaatcctga aaaaactctt ccaaaagcta tttttcttgc
     4261 tttaatatcc gcaaccattt tatatattgc agttgcctgg gttatcgtct tgaccatacc
     4321 atccaatact ttagtcaaaa cagacatgcc ccttattgaa attattaagc ttcagggaca
     4381 atctcccttg ttgttttcaa tcattgcatt aatttctatt accaatggaa ttctggtgca
     4441 aattattatg gcttcacggc ttatttatgg tatggccaat caacaaaatg cccctcaaat
     4501 attctcttct gtctattcca aaactcaaac accagtccac tcgaccttgc tggtatttct
     4561 tatgatttta ctctttgcct atgctctccc cattactacc ttagccaaat taaccagcag
     4621 tatcatatta tgtattttta tcatcattca tgcctcgcta attaaaatta aactgacaga
     4681 aaagaaacca acaaacgcca tttcattccc tattgttttc ccgatgattt cgattttatt
     4741 aaccctggcc tttttgatca tacagttttt tttaaagtag attatttttc ctaatttttt
     4801 aacctagtgc cacataaatt ttgggagtca acagagtcat tgtctgtttt caattcaccg
     4861 cgcctctctg cctgcatcgc tagtttataa tgcagcatga tctctcgagc ccgggctact
     4921 ggcttcgttg cctctgtttc tactgatgaa tttgattgcc gcgcaacttc gggccccttg
     4981 tcttcacgca taggcttcaa tgcatcaaat aaagaggaat cctgcttttt aatatgctca
     5041 aaaaacgcta tggcagaacg ggtttcccca taagccgcag gaagaaaatt gagtaaccct
     5101 tgtcttggcg ttgaagctgc tgttacaaaa ttccgtaaag cttcatggcg ttctgtccca
     5161 tttggtgcgt tttcaaatgc tgatttgcat tgaacaagag attccttttt atcatctctg
     5221 ccacttacaa aatgacttgt tcccatactg cttagcgcct cttgcatagc cactttgata
     5281 accattattt gttcaggcgt gaaacctcgc tcgtctttgc tgctgggttg aatcacctca
     5341 tcgcttgatt ggattgtagc ctctggcatt attcgggatt ctgaggaaat ctcggagagt
     5401 acatgcgtac tactgatgcg atgagattgt aataaagttg ctatcgcttc atgttttaaa
     5461 taatttgcaa gttctaatgg tgtaaatttt aaaacagtac cttccggttt tatttgatag
     5521 ctggctttta aatcaaattt agcccccaat tcaagcaaca ttatgacctg tttaatatta
     5581 ttacttaata ccgcatgatg aagtaatgtt ccatcagcaa tattaacaat ataagaagga
     5641 tgtgaaaaat tatcgaggga tctgattaat tgattaatct cagattctga cgctagtcta
     5701 attttttctt tatgaaatct atccggcaag caattaaata aagaagaaaa ctgatcagag
     5761 acacagacac attcttttgt cgttttattc acatagaaaa tttgattcgc tttggttgca
     5821 atataagctt cattggtggt aaatggcata gctagtaact tttccggctc agcttcttct
     5881 gcatagcata cgctacatgg tgcgagagac ttcctgctga aagaacaagc caattgttga
     5941 ttaatgtctc cataaggtaa attctcttca aacaattgac gattattgcg ttcaagccaa
     6001 aaaaacaacc ctggtaattt agccccccaa tatgatttgt aacttatttt ttggacaccc
     6061 aaatcttcga tttcaatata gggaacgtga ctaccactaa aacataatag atttccaaag
     6121 gatggatttc ttgcccatat atcaaaaatt tctgacattt gactatcgtt gcgttgagga
     6181 gaatacatta aataaaagtc atttgatttc tgaaatcctg atttagcagt agcaaccgta
     6241 tctctgccag gtaaattaat ttctctatct gttgccggaa atacattagt gtcaaggaaa
     6301 tatcctggtt ggcaaactaa taagaacaat gaaaataaat ctttgaacat cactctttgc
     6361 tttatatcgc tattttgtcc tgaaggaagg ttatcgttta gaaatttttg tacaaacagc
     6421 gcttgatcac gcaactctcc ttgagactcc tgtcttatta tctcttctat tccgcatacc
     6481 tctatagtca cgccagcgtc attaaagagt ttttgataga aatcttgatg ttgctccaaa
     6541 caccaaaatt taatggggtt aatcggttta ccctgagctt gcgattgaag tgcttttgcc
     6601 attttgatag gaccggcaac atcgtgacca gcaatgctcg cagacgaatt cattttagta
     6661 ggtaatccta cccacaaata atgaataact gacagttgat cgttgtccat gataatctcc
     6721 aaatgattca tacatttcca gtataaaaaa acattattag ggcaatgtga tatcaaaagg
     6781 aatgttggta atacctgttt ccaaacagga attccaatag atatagacca gcttttagat
     6841 tatttttaca tagcaaacct tcaaaagttg ttgtgctata ctttttataa aaagataaag
     6901 aaaatcttat ggatattaga gacatcaaat cctttctcgc tgtcgtcgaa taccattctc
     6961 tcaccttggc agctaaaaaa ttgcatgtca ctcaatctgc catgagcaaa cgcattcgaa
     7021 aaatagagga cgaattaaat atacggttga taattagtga aggctcgagg ttaattttta
     7081 ccgaagcagc caagcaactt atcccttatg ccaggcaaat gctttccgca catcaaaata
     7141 tggttcacac cctgaaaaac tcgcaacaat tcacgcaaag catcatcata ggggcttccg
     7201 tgtatgtgtc tcactacgta ttgccaggct tttttaaata cttaaaggaa acccatccga
     7261 ccattcaagc acatctgaaa accattagtg aaaaggaagt cgagcatatt ctaaaccacg
     7321 gcacagttga tctagtaatc tgcccggcac gggaagcatc tgaaaaatta ttgagcaact
     7381 cattgctctg ggaagaaaaa ctgaatttgg ttgtggcagt aaatcatgag ctagcccata
     7441 gaacttccgc actttcactc tcagaactat cagagtaccc ggccctctta actgagaaag
     7501 gagcggcatt gcgtgacaag cttgataccc tatttgaaaa aaatcaactg ataataaacc
     7561 tgggttttga aatgagcact atcgatgcca taaaatctct agtagagtac ggtttaggct
     7621 ggagctatct gcctgatagt ttgttgtcag aaaaattaaa aatcttaaaa ataaaagacc
     7681 ttgagctaac aatcccattt catgcttttt atctcaaaaa aagaatcagt gaccgcttga
     7741 ttaatgacct gctatgctat tttaaaaaat ggcaaaatcg ggataattaa caggaatgaa
     7801 tccaggacga aaataaccgt ctttatcaaa atcgaaacaa cagaccgttg ttaatacgtt
     7861 tttacaacac taacattgca atagactgaa tctccgcttc cttgatcctt ttgaacatat
     7921 actataatta atcataagat aaatccgaga cacagcaaca tgatgaaaat tgaatcattt
     7981 caatacattc aaaaccaggg atggagtatt ccaacatttc ctgcactcga ctccgaaaat
     8041 accttaattt tagtttttgc cagccccatc tttagggcaa acagcaaacc gattgaagaa
     8101 ttggctcaag cctataaaaa atcaaaaatc ataggatgct ccactgcagg agaaattttt
     8161 gggcctaata tttttgataa cagcatttcc gttgcagtag ttcaatttga gtcaacaaaa
     8221 cttcaattgg caaaaacaga ggttaaaagc actgaggaat cttatgaggc gggagttcat
     8281 ttatcaaaat cattgcatca aaagcaaaat ccccaaagta tatttgtttt gtccgatggc
     8341 ttaaatgtca acggttctga attaattaaa ggattaaacc agcatgctgg caaaggggtt
     8401 actattacag ggggtctggc aggagatggt gcggatttta aaaatacctg gattatttat
     8461 gatggaaaaa ttgtagacca ttgcattggc gcagtggggt tttatggtga tcaaatccat
     8521 gtaggacatg gctcaagagg aggatgggat atttttggac cggaaagagt tattactcaa
     8581 tctaaagcaa atattctcta tgaactggat aaccagcccg cactaaaact ctataaaacc
     8641 tatcttggtg acagggcttc tgaattacca gcgtcaggct tgctttaccc acttgctatc
     8701 cgtaacccat ctgcaccaga ttctcagcat attgtgcgca ctattttggg agtcaatgaa
     8761 gaagagcaat ccctcatttt tgctggcgat ttgcctaagg gatactatgc tcaattaatg
     8821 cgtgccaatt ttgaccgcct cattgtcagt gccaatgaag cgggagaaaa cgctaatgat
     8881 agacttttta aaaatggaag cttgaataca caacctgttt tggcaatcga tatcagttgt
     8941 gtcggtagaa gattggtatt aggtgaactt acggaggaag agaccgaaac aacattacaa
     9001 tcgctccctc ccaaatcgat gcaaattggc ttctactctt acggcgaact atcgcctctg
     9061 gcaacaggta cctgcgagtt gcataatcaa accatgacca ttacgaccct gttagagagt
     9121 ccataaacca aaaaggaatt cagtaataat gacagaaatg catcggttgt tgcagcggca
     9181 gttaaaaaaa tttggtttta ctgctgatgg attaccacaa aatctggaga gttggactca
     9241 atttattgca catatcaata aaacatacta cgaaaatgat caagaccgat atttattaga
     9301 gcgctctatt gatatttctt ctaaagaaat gagggatatc aattcgaaac tcgaatacgc
     9361 ccaggatata gctcaaatgg ggtattggca ttatgacgcg gacacagata ccgcaacatg
     9421 gtccaaaggg ctatttaaaa ttctgggttt gccgcctagt aatgtggcac taaactattc
     9481 ttcattttta gatcttgtgc atcctaatga taaagcagga ttaaaaaatg tggtcgaaaa
     9541 ggccctcaaa gaaaaagaag attatgatta tgagcttaga gtaagaaacg gggacaatga
     9601 ttataagtgg taccgaacga tagcaagagc caatcctcat aaaaacgaaa tttccggggt
     9661 tttaatggat atctccaaaa ggaaaattac tgaagagcaa attcatgaac tgaataataa
     9721 actggtttca acagcacgtc ttgccggtat gtcggaagtg gccacctcga tacttcataa
     9781 tgtgggcaat atattaaaca gcgtcaatgt ttctgccatt attctgaaaa agaatatatc
     9841 caaaattcat tataaaaaac tgtccgctat aaaaaccatg cttgacgaaa acatcaatca
     9901 attaagcgaa tacttgttaa ataatccaaa aggtaaatta attcttccct atttggacgc
     9961 gctatcgaaa accctgcatg aagagtatga agaaaacatg aaagaagcca atagtctgga
    10021 aactaacatt aatcatatta aagaaatcat agccatgcag caatcattga gcggcttatc
    10081 gaatttaaca gagaaaattt ccatccatga tgtgattctt aatgcggtaa aatcagtcat
    10141 attggacaaa catcaaattg atgtccgtat tgagattgaa ccggaaatcg atgaatttat
    10201 cactgataaa tccaagttgt ttcaaattct tactaacata ctcgctaatg caaaagattc
    10261 tgttttacta cacgaagcga atgattccag gacaattgtt atctccattg ggagaactga
    10321 aaaaaatcat attatgatta aaataaatga taatggaatt ggaattcaaa aagataatct
    10381 ggatcgaatt ttttcttttg gatttacaac caagaaaaat ggtcacggtt ttggactgca
    10441 ttgttccgca atttatgcga gagatctggg tggtacactt tatgctgaaa gtaatggcaa
    10501 tggcaaagga gctcaatttg tattagattt gcccatcaat cccaaaacct tgagtacgag
    10561 gtagaaatga gtactgaatc ttttcgcatt ctggtcattg atgacaaccc ggcaatccat
    10621 caggatttta tgaaaatcct aaatgtcagc aagaactctg tattattaaa taaatttgat
    10681 gtcgagctgt ttgatgatga gacagaacaa cttggcattt ctttgcctga atttgaaatc
    10741 gatgtagcta ctcaaggcat tgaaggcata gaaaaagtga aacgttcact tgaagaaggc
    10801 cggccatacc cactcgcctt tgttgacata agaatgcccc ctgggataga tgggatagaa
    10861 accatcaagc gcatttgggc cattgacccg caaatacaga ttgtaatatg cagtgcctat
    10921 tctgattact catgggaaag cacagtgaat accctgggtt taagtgataa tttattggtg
    10981 ctaaaaaaac cctttgatgt gattgccgta agacagttgg caagtgcttt gacacaaaaa
    11041 tgggtacttg ccagggaaac tcaaaaacac actaaatttt taaatcaatt ggtagaagaa
    11101 cgaaccgaat ctttgcagca atccttctcc ttattgagag caacgattga gtcctcttcg
    11161 gatggcattc tggttgtcga tttacaagga aaaatcgttg attacaactc tcaattcgtc
    11221 aaattatgga atatacccga atccatcata aaaaaagaaa ccagtgagcc ccaactcatt
    11281 cagtatatgt caactcaatt actcaattca gaacagcatg cccagcatgt taaagaattt
    11341 tctattcaca tcgatgattc cagcatgcaa atttatcatt ttagaaatgg taaagtggtt
    11401 gaatgctgtt ccaaaccgca tcgcgttggt ctgcaaacgg taggccgtgt atggagcttc
    11461 agagacatca ctgaacaaac gcgattaaag gaaaaactgg agtatcaagc cactcatgac
    11521 gcattaacca atttacccaa tcgcttgtta ttaattgatc gtattgaaca tgctatcagt
    11581 ggctatgccc gccataaaat gaaatttgcc atattatttt ttgacttgga ccgctttaaa
    11641 atgatcaatg acagcttgag ccatgaagca ggcgatcaat tactgtgtga cgttgctgaa
    11701 cgattacgat ctttggtacg aaaagaagat actctcgcca gacttggtgg ggatgagttt
    11761 gtaatgcttt ttcaatcgtt taattctgaa gagcaaattg ccggagtagc tcaaaaaatc
    11821 ctcaaatcct ttcaaaaacc atttcatatt gctgaaagag acatcaatat tcatgtcagc
    11881 ataggtatta gcgtttatcc aacggatggc aaaacagtaa acactttact cagtaatgcg
    11941 gatatggcga tgtatcaggc aaaatttcga ggtggtaacc aattctcatt ttatacagag
    12001 aaactcaaca aaaaaacaaa ccagcaattt cagctggaat tagacttgca aaaagcgatt
    12061 gtccatcatg agtttttcct cctttaccag cctcaattta cagttaatac tcatgagatt
    12121 cgttccatgg aagcattaat ccggtggaat cacccgcaaa aaggtattat tttacccttg
    12181 gattttattc ctactgccga aagttcaggg cttatcgtac cgataggaga gtgggttttg
    12241 cgggaaacct gtaagcaaat tggtgcgtgg cgcacaatgg ggctgcctga cattcgtgtc
    12301 gcttgtaatg tagcaagtaa acagttaaga caacgcgact tccctgcaat ggtggaggat
    12361 cttttaaaag aatattcagt cgaacctcat tgtctggagc tggaaattac tgaaaatgtg
    12421 attattgata aagaaataca aagaacgatc aaaaaattga aatcactcgg tgtgttgatt
    12481 attttggatg attttggcac aggaaattcc agcctgaatt tattaaaaca ggtcgctgtt
    12541 gatagcctaa aaatagacca atcattcatt cagaatattt caaaatcgcc aggagatgaa
    12601 gcgatcatcg atgcgattat tgctatcgca caaagcatga attttaatat catcgccgaa
    12661 ggagtcgaaa cgcagaacca gcttaaattc ctcaagaaaa ggcggtgtaa tgacattcaa
    12721 ggctttttaa tgagtaagcc tataccacca gaagagatag aaaaacttct gcaaggaaat
    12781 aggacaaaat aaggtaaaaa tcgcattggc atctataaga gctatccgat ggattgcaat
    12841 tatagatgca agacaaacta caccgtaatc ataacggttt gattacgtaa atcgttctca
    12901 tcataatcca tcacatccac atccatgact tcgatatcca ttggttctgg actcaaatgc
    12961 ctccccctcc ttctggcttc cttaaagaaa ccatagtttg cctcacctga acaatgataa
    13021 ataaccttcg gtttatgatc ttctatagcc tgttcgagct cacgtatcaa tttcatacca
    13081 ctatggtgaa gttggtacac atcatccgtt tgcagttctt ctacaagcac tggtttcgga
    13141 tcttcatccg tattttcttc catgtatgga gtatcaagat ttgcaaaaat tttggaagta
    13201 taatcaccaa tagatttggc ttttaccaaa gtacaattca tttctctcaa cttccaggag
    13261 atgtcatcaa acacatctgg gcttgagcac tcacttttta ccacgtcaat ctcggaaagc
    13321 atttcaacaa ttctctcatt ttgaaaagga gataatttga gatctgattg attttctaat
    13381 agcgtcgatt caacaaagct taccagatgt tctgaattct tatcctcatg aaactgtttc
    13441 gcaatagggc caccatgcgt tgtgataagt tcttcatact ctcttaaaag agtcgtgtat
    13501 tcgaatatac tttgcgggag ataaacatta ctcatataat cataataagc tttagaccag
    13561 gttgaatggt cattttgtaa ttgattaaga aaccgattat gttctatatc caactgacgt
    13621 aacttctcct gaattctttc aaaatcgctg acagagttat cgtaagcgtc tttagtatat
    13681 atctgaaccg tgagaagtga cggtaatgcg ggtatcaaag cagttttgaa ttgtcttttt
    13741 aaattagtgt gttcattgta tgtagttgag agattgaata atcgcttaaa tttttcttca
    13801 caatctttaa aaatcaggcg actctcggag ctcaattgct tccgatcaat agagttaggg
    13861 aggttattag ctat
//
LOCUS       DAXLDT010000079        13626 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXLDT010000079 DAXLDT010000000
VERSION     DAXLDT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.079"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..368
                     /locus_tag="ACF2SP_001868"
     CDS             <1..368
                     /locus_tag="ACF2SP_001868"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFD2359651.1"
                     /translation="NPNTLTWKAISANGKVIRTGRGSGGKHYCSDVRRSCKTPSGVFR
                     VISKGGPACRSSRYPLGKGGAPMPYCMFFSKLYAIHGSPDVPNYNASHGCVRVKPHDA
                     RWLSQNFIKIGTKVIIKSY"
     gene            487..1890
                     /locus_tag="ACF2SP_001869"
     CDS             487..1890
                     /locus_tag="ACF2SP_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359652.1"
                     /translation="MIEVNIWLSTASLFKQRVKHKFFGPLLASHEKGENVGHVNFNIE
                     IDERVKESFDFIEQHASELKVKKTLRTIPTQATGASRSDIESSHLKPTMVKSDVVSHS
                     FWPENRPTKSETIIGKRVKPEFNTHEDDMIAEDSLAPMSITHRKSALEEVDKEKKMNL
                     DLLIEISDLEVNMEQRLLFTDDLKKLHLEKEELSKQQKQLTSTYQTELKDLKRQLARL
                     ELYLNKINGQITATERTLGYLNKLENPDPKTRAQSIELTEKLIKLKEERETTISSQHE
                     ISKLISKSEFTYNESMQKIEKKLQQVELAIKHREENLRQLDIKINGRDENDLKELREE
                     AYRHKEFTSRKEQFIKSRDFTEGRHPDYSVTLPTVESGLTYYVDEVKILKAMQEERKQ
                     NYSIVFYNCASSAKRCLLAGIDDNLRAKLKETGLDSKFFEIRKIETCQSLRNWARTLE
                     SKLTELNFPSPGPTRVV"
     gene            complement(1956..2348)
                     /gene="erpA"
                     /locus_tag="ACF2SP_001870"
     CDS             complement(1956..2348)
                     /gene="erpA"
                     /locus_tag="ACF2SP_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFD2359653.1"
                     /translation="MASVDVSQNTSDIHFSVSAADKVAELIKEEDNSNLNLRVSITGG
                     GCSGFQYGFSFDEQINDDDTIIIQQCSDGKSSVKLLIDSMSYQYLHDAEIDYIKGIQG
                     EQFVIRNPNAKTTCGCGSSFSIGDEDVL"
     gene            complement(2483..3568)
                     /gene="mnmA"
                     /locus_tag="ACF2SP_001871"
     CDS             complement(2483..3568)
                     /gene="mnmA"
                     /locus_tag="ACF2SP_001871"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFD2359654.1"
                     /translation="MKAKVIVGMSGGVDSSVAAWLLKEQGYQVEGLFMKNWEQDDHND
                     FCPAAKDLADAQAVCNQLRIPLHTVNFSKEYWDRVFAYFLSEYEKGRTPNPDVLCNKE
                     IKFNAFLNHALNLGADYIATGHYAKNTIEGSIGYLFKAKDREKDQTYFLHAVEPEALS
                     KTIFPIGDFTKPQIREFAKQLGLVTHAKKDSTGICFIGEKRFKTFLNEFILAKPGEIK
                     STGGKTLGQHDGLMFYTLGQRQGLGIGGLQNSTDEPWYVVDKDIASNTLYVAQGSQHP
                     MLYSQGLICGPIHWLADYENHLPLTCFAKTRYRQTDQACMISPPDNNLHYVIFSSPQR
                     AITPGQFIVFYEKNQCLGGATIEQIIR"
     gene            3838..4266
                     /locus_tag="ACF2SP_001872"
     CDS             3838..4266
                     /locus_tag="ACF2SP_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFD2359655.1"
                     /translation="MMHLQELAKHGQQEKEVILNERLPSFVSPGCHLHASYHIEAKDD
                     FYLIHLRVQGNLNSICQRCMENFTVCYNNSTVIAVCRSDERAEQLLSQYECLVSSNWQ
                     VDLNELIIDELHLYAPQVHPDINECANEINEILTGKKQTS"
     gene            4352..4543
                     /gene="rpmF"
                     /locus_tag="ACF2SP_001873"
     CDS             4352..4543
                     /gene="rpmF"
                     /locus_tag="ACF2SP_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFD2359656.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTKPTLSVDPTTGETHLRHHMTP
                     DGYYRGKKIIDAETAYEQE"
     gene            4549..5577
                     /gene="plsX"
                     /locus_tag="ACF2SP_001874"
     CDS             4549..5577
                     /gene="plsX"
                     /locus_tag="ACF2SP_001874"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFD2359657.1"
                     /translation="MKNITIAIDAMGGDHGLEIVIPACIRAIKNNPDLKLLLVGVQDK
                     ISASLKKHGMLSCQQFTIVHASEVVTMDELPSHALRNKKDSSMRIAINLVKEGRAQAC
                     VSAGNTGALMATARYVLKTLPGIDRPAIVSELPTMGGKTRVIDLGANVDSCAEHLFQF
                     AVMGSALIQAIENKPKPKIGLLNIGVEEIKGNDQVKRTAHMLAECSVMNYVGYVEGDH
                     FYSGDVDLVVCDGFVGNVALKASEGLAKLLLTVLKESFSRNWLTKIAGLIALPALKHL
                     KNRLDPSRYNGASLLGLNGIVVKSHGGANEVGFQHAIEQAVLEVKNNVVDLVRDQIND
                     FINQGLLL"
     gene            5574..6527
                     /locus_tag="ACF2SP_001875"
     CDS             5574..6527
                     /locus_tag="ACF2SP_001875"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFD2359658.1"
                     /translation="MKNAVINGTGSYSPERQMTNAELETMLDTSDEWIVTRTGISSRS
                     VAQEHETTSYMASRAAEQALEASGLDAEEIDLILVATCTPDYFFPSVACHVQHALGIK
                     RPIPAFDIGAACSGFVYAMDVAKQYIATGAAKHVLVVGSESMSRAVDWTDRSICVLFG
                     DGAGAVVLSASDRQGIMGSVLHSAYDSDKLLVLRNSTFEQDRATIGMRGNEVFKIAVN
                     IMGNIVDEVLEASHLKKSDIDWLIPHQANIRIIQAIAKKLSLPMSHVIVTIGNQGNTS
                     AASIPLALDYSIKNNRIKRDEILLIESFGGGMTWGAMVIRY"
     gene            6554..7501
                     /gene="fabD"
                     /locus_tag="ACF2SP_001876"
     CDS             6554..7501
                     /gene="fabD"
                     /locus_tag="ACF2SP_001876"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFD2359659.1"
                     /translation="MVKSAFVFPGQGSQSIGMLSDFMPQYTVVTDAFAEASEALGYDL
                     WDLVQNGPETKLNQTEYTQAAMLTADVAIYRLLMQLGVPQPQVMAGHSLGEYAALVCA
                     NSLSLKDAAQLVARRGQVMQNAIPLGEGAMAAIVGLSDEQVRSLCEQASTANHLVTPA
                     NYNALGQIVVAGHRVAVDKLIQLAEETGARLAMIIPVSVPCHCPLLKNAAELFAENLA
                     QIEFQIPSVDVISNVDLSIYHSTQHIRDKLKEQLYSPVRWVETVQLIQQRGIELVVEC
                     GPGKVLNGLIKRIERNLTTISVYDTISLDQVIERLHIPV"
     gene            7513..8259
                     /gene="fabG"
                     /locus_tag="ACF2SP_001877"
     CDS             7513..8259
                     /gene="fabG"
                     /locus_tag="ACF2SP_001877"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFD2359660.1"
                     /translation="MINLEGKIALVTGASRGIGRAVAEKLARNGAFVFGTATTEQGAA
                     SINSFFEKEKLAGKGIVLDVTSTEQVENLMSTLSDNDQCPSILVNNAGITADNLLLRM
                     DDEEWYKVIETNLNSIYRMTKACLKPMFRARWGRIISIGSVVGASGNSGQVNYTAAKA
                     GVIGFSKSLAQEIGSRGITVNVVAPGFIDTDMTTALPDMVKEEMLKRIPMRKLGQPED
                     VADAVAFLASDSAKYITGETIHVNGGMYMS"
     gene            8344..8592
                     /gene="acpP"
                     /locus_tag="ACF2SP_001878"
     CDS             8344..8592
                     /gene="acpP"
                     /locus_tag="ACF2SP_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFD2359661.1"
                     /translation="MSTVEERVRKIVVEQLGVKEEELKNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEKAEKITTIQEAIDYIESNLNKEEA"
     gene            8612..9850
                     /gene="fabF"
                     /locus_tag="ACF2SP_001879"
     CDS             8612..9850
                     /gene="fabF"
                     /locus_tag="ACF2SP_001879"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFD2359662.1"
                     /translation="MNSRRVVITGMGMLTPVGLNVEETWRSILAGVSGVGLVEDFDTT
                     EYPTKIWAKVKNFNVENHMPIKDARKMDVFTQYGMAAADEAMLDSGLKIDETLSRRIG
                     VAVGAGIGGIQTITNNQDKLVAGGPRKVSPFFIPAGIINMVAGQISIKHNLKGPNISV
                     VTACTTGTHNIGLAGRIIAYGDADVMICGGAEMTTTPLCLAGFSAVRSLSKRNDEPEK
                     ASRPWDKDRDGFVMGEGAGILVLEEYEHAKARGAKIYAELVGFGMSGDAYHITAPDED
                     ADGASRAMEAAIQDAGIDPRQVDYINAHGTSTYLNDLNETKAIKRVFQNHAYDLAVSS
                     TKSMTGHLLGAAGAVEAIISILAIRDQVAPPTINLDNPDEGCDLNYVAHVPQSRTINY
                     VLSNSLGFGGTNGSLLFKRI"
     gene            9851..10849
                     /gene="mltG"
                     /locus_tag="ACF2SP_001880"
     CDS             9851..10849
                     /gene="mltG"
                     /locus_tag="ACF2SP_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFD2359663.1"
                     /translation="MQFSRHKKLVILVLSLFFMSFLFLFWNIYRLLYSPIIPKTASPM
                     IIALDKATSAYQFAYILQDKKLIHSAKFFLLIIRFEGLSHQLKAGVYQITPGETAMKL
                     LHRVVAGDVITQNFTIIEGSTQQKVDYDLRQANYLKYNPEDWAIVKENYPSAEGLLLA
                     DTYQYQGGSSSRTLLEQAHRNLLNYLNTSWTNRAPNLPYKTAYELLIAASIIEKETAI
                     AQEKKLISGVMVNRLKKKMPLQMDPTVIYGLGNQYTGKLTHNDLLIQSPYNSYLNRGL
                     PPTPIAMVGKEAIDAAAHPQLSNYLYFVAKGDGTHQFSETYEQQRQAINQYRRKDY"
     gene            10849..11487
                     /gene="tmk"
                     /locus_tag="ACF2SP_001881"
     CDS             10849..11487
                     /gene="tmk"
                     /locus_tag="ACF2SP_001881"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFD2359664.1"
                     /translation="MSSLTGKLIVIEGLEGAGKSTAVNLVVELLSQKKISTITTREPG
                     GTRIGEILRSIIKNPEYNNVLDDRSELLLLYAARIQLIEQVIKPALNVGQWVIADRFE
                     LSTLAYQGGGRKMNMRVINELSNFCLKGFKPDLTLYLDINPELGMIRAKSRGKFDRIE
                     QESIEFFHRIHDTYHVLVKQNPEIMMIDANRSLDEVQSSIQSVIEEFIEHNL"
     gene            11484..12389
                     /locus_tag="ACF2SP_001882"
     CDS             11484..12389
                     /locus_tag="ACF2SP_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFD2359665.1"
                     /translation="MITYQEQWQKMQMALAVRRIPQAMLFLGSLHCGLIEFTNQVIKL
                     LLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERSGGIIKIDQIRGLQSTAFLTPQR
                     TNHKLIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQMTQF
                     AVFDELPYNNLLALGEYYTQESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQH
                     ELGAFLWFLYLLYSQLQYMHLFRVAYSGTLKEQLNKLSSLLNPVMIFAQVDKINTLLR
                     KLSHNMNMNHTLVLEDLLFSLVVDY"
     gene            12515..12856
                     /locus_tag="ACF2SP_001883"
     CDS             12515..12856
                     /locus_tag="ACF2SP_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFD2359666.1"
                     /translation="MTEKPQLINCSFPNEASLYMAYMPFVKGGGLFIRTQHVYSLGQS
                     LELSVKLLNENEPYLVTGKIVWITPKGAQGNKPPGVGIQFTGENSRYLCNKIETYLAG
                     MLKSCQVTDTI"
     gene            12916..>13626
                     /locus_tag="ACF2SP_001884"
     CDS             12916..>13626
                     /locus_tag="ACF2SP_001884"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFD2359667.1"
                     /translation="MLVDSHCHLNFIDLTHFNHDLAQVINQARENGVEHFLSVCVELS
                     DYPQLERLAKDYPDISISVGVHPNSEMNYPVTAQMLCELAQNPACIAIGETGLDYYRN
                     PTEEAQSLQRDRFREHIKAALITSKPLIIHTRQAAKDTLLMMAKEGASQIGGVMHCFA
                     EDLDIAQRAIDLNFYISFSGIVTFKNAVSLQDVARQIPLEKILIETDSPYLAPVPHRG
                     KQNHPALVKHVAETIAQLR"
BASE COUNT         4307 a         2284 c         2855 g         4180 t
ORIGIN      
        1 ttaatcccaa tacattaacc tggaaagcca tcagtgctaa tggaaaggtg atacgtacag
       61 gacgcggatc aggtggaaaa cactactgtt ctgatgttcg cagatcatgt aaaacaccgt
      121 ctggcgtttt tagagtgatt agcaaaggtg gaccagcatg ccgatccagt cgttatccgc
      181 tgggtaaggg tggagcacct atgccatatt gtatgttttt tagtaaattg tatgcaattc
      241 atggttctcc ggatgtgcca aattataacg caagtcatgg ttgtgtcaga gtgaagcctc
      301 atgatgcaag atggttaagt caaaatttta ttaaaattgg aacgaaagta attataaaat
      361 cttattgata acttaatctc atgtattata gttgagaaat atgtagtata attatatttg
      421 ttggaaaatt ttttaacaaa ataagtttgt attttattat tttattattt tataagaagt
      481 atcattatga ttgaagtaaa catctggcta tcaacagcgt ctttatttaa acaacgtgtt
      541 aaacacaaat tttttggacc gcttttagct tctcacgaaa aaggagaaaa tgttggacat
      601 gtaaatttca atatagaaat tgatgagcgt gttaaagaaa gttttgattt tattgagcaa
      661 catgcttcgg agcttaaagt taaaaaaacg ttgcgcacta ttccaactca agcgacaggg
      721 gcttcccgat cagacataga gtcgtctcat ttgaaaccaa ctatggtcaa gagcgatgtg
      781 gtttctcaca gtttttggcc agagaatagg ccaactaaat cagaaaccat tataggcaaa
      841 agagtaaaac cggaatttaa tactcatgaa gatgatatga ttgctgaaga ttcactggct
      901 ccaatgtcca ttacacatcg caaatctgct ttagaggagg ttgataagga aaaaaaaatg
      961 aatcttgact tgcttattga aatttcagat ttggaagtca atatggagca aagattactt
     1021 tttacggatg atcttaagaa attgcatttg gaaaaagagg agctgagcaa gcaacagaaa
     1081 caattaacca gtacttacca aaccgaatta aaagatctaa agaggcaatt agcgaggttg
     1141 gaactttatt tgaataaaat aaatggacaa attacagcta cagagagaac tttaggctat
     1201 ttgaacaaat tagaaaatcc agatcctaaa accagagcac agtcgattga gcttactgaa
     1261 aaattaatca aattaaaaga agagcgtgag actacaataa gttctcaaca tgagataagc
     1321 aaattgatat caaaatctga attcacttat aatgaaagca tgcaaaaaat tgaaaaaaaa
     1381 cttcaacagg ttgaacttgc aattaagcac cgtgaagaaa atctaaggca attggatatt
     1441 aaaatcaatg gtagggatga aaatgattta aaagaattgc gagaagaggc gtatcgccac
     1501 aaagaattca cctctcgcaa agaacaattt atcaaaagcc gagattttac tgaaggccgg
     1561 catcctgatt acagtgttac tttaccaact gtggaaagtg gtctaactta ttatgttgac
     1621 gaagtaaaaa tactgaaagc tatgcaagaa gagcgtaagc aaaattattc gattgttttt
     1681 tataattgtg ctagtagtgc taaacgttgt ttattagcag gaatagatga caatttgcgg
     1741 gccaaattaa aagaaactgg tttggatagt aaattttttg aaataagaaa aattgaaaca
     1801 tgccaatcat tacggaactg ggcaagaact cttgagagta aattaacgga acttaatttt
     1861 ccatcacccg gacctacccg agttgtctaa gaataataag cagttcatcc gtgaaaaaac
     1921 taagatcact gcccaatatt taggcagtga tttttttata aaacgtcttc atcaccaata
     1981 ctaaacgaag agccgcaacc acaagtcgtt tttgcgttag gattgcgtat gacaaattgc
     2041 tctccttgaa ttcctttaat gtaatcaatt tccgcatcat gtaaatattg gtaactcatt
     2101 gaatcaatga gcaatttgac agaagatttt ccatcagaac attgttgtat gataatcgtg
     2161 tcgtcatcat tgatttgttc atcaaagcta aatccatatt gaaatccaga acaaccacca
     2221 cctgtaattg agacgcgtaa attcaaattg gaattatctt cttctttaat taattctgct
     2281 actttatcag ctgcgctgac agaaaaatgg atatctgaag tgttttggga tacgtcaaca
     2341 ctggccatac ttagctccaa aataattact accattatta ttcattatat caataatgtg
     2401 agttgtttcg tagtctcaat ccataaattg gtatggggac cagagaaaac aaacagtacc
     2461 agtacaaaat ttgcttccct gtttagcgaa taatctgctc tatagtagcc ccacccaagc
     2521 attgattttt ttcataaaag acgatgaatt gtcccggagt aatcgcacgt tgaggacttg
     2581 aaaatatcac atagtgcaga ttattatccg gaggagaaat catgcaagct tgatcggttt
     2641 gcctatatct tgttttggca aagcaggtta gtggtaaatg attctcatag tctgccagcc
     2701 aatgaatggg accacatatc aaaccttgtg aatagagcat tggatgttga ctgccctgtg
     2761 ccacgtaaag ggtgttactg gctatgtctt tatccacgac ataccatggc tcatctgtcg
     2821 agttttgtaa tcctccaatt cccaaaccct ggcgttgccc taatgtgtaa aacattaaac
     2881 catcatgttg accaagtgtt tttcctccag tactttttat ttctcccggt ttggcaagta
     2941 tgaattcatt aagaaaagtt ttgaatcttt tttcacctat aaagcaaata cctgtagaat
     3001 cttttttggc atgagtgaca aggcccagtt gtttagcgaa ttctctaatt tgtggcttgg
     3061 taaaatcacc tattggaaaa atggttttgg acaatgcctc tggttcaaca gcgtggagga
     3121 aatacgtttg atccttttct ctatcttttg ccttaaataa atagccgata gatccttcta
     3181 ttgtattttt ggcatagtgg ccagtagcta tataatctgc gccaaggttt aaagcatgat
     3241 tcaaaaaggc attaaatttg atttctttat tacataacac gtcggggttg ggtgttcttc
     3301 ccttttcata ttcactcaga aaataagcaa agactctatc ccagtattct tttgaaaaat
     3361 ttacagtatg tagtggtatg cgcaattgat tacagacggc ttgggcatct gccaaatctt
     3421 ttgctgcagg gcaaaaatca ttgtgatcgt cctgctccca gtttttcatg aatagccctt
     3481 ccacctggta tccttgttct ttcaatagcc aggctgctac agaagagtct actcctcctg
     3541 acataccaac aataacttta gctttcataa atggtttgta tcgctctttt ttgaattata
     3601 atcatattgg aatgtgcgat tttaacaaat taactacact gtaggaaatt gtaatttagc
     3661 cctgtattta ttacccaata agttgatatt ttttacatat aattggataa taccacgttt
     3721 ttagggattc gttatcattt taacttgatt atgttagtaa gtcataaacc gtctataagt
     3781 gcttattact tgcaagactc aaagagcaaa ctcattctat atttcggata attatctatg
     3841 atgcatttac aagagttggc aaaacatggc cagcaagaaa aggaagtgat tttaaacgag
     3901 cgtctaccct cgtttgttag tcccggatgc catttgcatg catcttatca tatagaggca
     3961 aaagatgatt tttatcttat tcatcttaga gtgcaaggta atttaaacag tatatgtcag
     4021 cgatgtatgg aaaacttcac tgtatgttat aataactcca ctgttattgc tgtttgtcga
     4081 agtgatgaaa gagcagagca acttttgagt caatatgaat gcttggtttc ttcaaactgg
     4141 caggttgact taaatgaatt aataattgat gagctgcatt tatatgcacc acaagttcat
     4201 cctgatatta atgaatgtgc taatgaaatc aatgaaattt taactgggaa aaaacaaact
     4261 tcttgatatt ttgataatta atacttggat tgaagttaaa atatcggtaa tattcccgct
     4321 ttatgaatgc aatttgttta ggagtaatac aatggccgta caacaaaata aaaaatcacg
     4381 ctcacgtcgt gatatgaggc gttctcatga tgcactaacc aagcctactc tatcagtaga
     4441 tccaacaact ggtgaaaccc atcttcgtca tcacatgaca ccagatggat attacagagg
     4501 caaaaaaatt attgacgctg aaaccgctta cgaacaagag taactttctt gaagaatatc
     4561 accattgcaa ttgacgcgat gggtggggat cacggcttgg aaattgtgat tcctgcctgt
     4621 attcgtgcta ttaaaaacaa ccctgatttg aaactattgc ttgtaggagt ccaggacaag
     4681 atcagcgctt ctttgaaaaa acatggcatg ttgtcctgtc agcaatttac tatcgtccac
     4741 gcatcagaag tggttacaat ggatgaattg ccatctcatg cattacgaaa taaaaaagat
     4801 tcctcaatgc gaatagcaat taacctggtt aaggaaggca gagcgcaggc ttgcgtcagt
     4861 gcaggcaata caggtgcctt aatggctacc gcccgatatg tcttaaagac tttaccgggg
     4921 attgatcgcc cagcaatagt atccgagtta cctaccatgg gtggaaaaac aagggtcatt
     4981 gatttgggag ccaatgttga ttcttgcgca gagcatttgt ttcagtttgc tgtgatgggg
     5041 tctgcattga tacaggcaat agaaaataaa cccaaaccta aaattggttt acttaatatt
     5101 ggtgttgaag aaatcaaggg taacgatcag gttaagcgta ctgcacatat gctggctgag
     5161 tgttctgtta tgaattatgt gggatacgtt gagggcgatc acttttacag tggtgatgtt
     5221 gatttagtgg tctgtgatgg atttgttgga aatgtggcat taaaagcaag tgaaggtttg
     5281 gcaaagcttt tattaactgt ccttaaagaa tcatttagca gaaattggtt aacaaaaatt
     5341 gctggtttga ttgctttacc agccttaaaa catttaaaaa atcgtttgga tccatcgcga
     5401 tacaatggtg ctagtttact cgggttgaat ggtatcgttg tcaaaagtca tggtggagcc
     5461 aatgaagtgg gttttcaaca tgctattgaa caggctgttc ttgaagtaaa aaataatgtt
     5521 gtcgatttag tgcgtgatca aataaatgat tttataaatc aaggattgtt gttatgaaaa
     5581 atgctgttat taatggcact ggaagttact ctccagagag acaaatgact aatgctgaac
     5641 tggaaaccat gcttgatact agcgatgaat ggatagttac caggactggt attagtagtc
     5701 gtagtgttgc tcaagaacat gaaacaacat cttatatggc ctccagagca gcagagcaag
     5761 cactagaggc atcaggcctt gatgctgaag aaattgattt gatattagta gcaacatgta
     5821 ccccagatta tttttttcct agcgttgcct gtcacgtaca acatgcttta ggaatcaaaa
     5881 gacctattcc ggcttttgac attggagctg catgcagcgg ttttgtttat gcgatggatg
     5941 tagcgaaaca atacattgct acaggggctg ccaaacatgt tcttgtcgta ggcagcgaga
     6001 gcatgtcaag agcggtagat tggactgatc gttctatttg tgtcttattc ggagatggcg
     6061 caggcgctgt tgttttaagt gcaagtgatc gccaagggat tatgggtagt gttttacatt
     6121 ctgcctatga ctctgataaa ttactagtcc ttcgtaattc aacttttgaa caagatcgtg
     6181 caacgattgg aatgcgaggt aatgaggtat ttaaaattgc tgttaatatt atgggtaata
     6241 ttgttgatga agtgttagaa gcaagtcatt taaaaaaatc tgatattgat tggctgatac
     6301 ctcatcaagc caatatacgc atcatacaag ccatagctaa aaaattatct cttcctatgt
     6361 cacatgttat tgttacaatt ggtaaccaag gtaacacatc ggctgcttct attcctttag
     6421 cacttgatta ttctattaaa aataatcgaa ttaaaaggga tgaaatatta ttaattgaat
     6481 cctttggtgg tggaatgacc tggggcgcta tggttattcg ttactaaatt aattaataag
     6541 gattaattct attatggtaa aatcagcatt tgtatttcct ggacagggat cgcagtctat
     6601 cggcatgttg tctgacttta tgccacagta tactgttgtc accgatgctt ttgcagaggc
     6661 ctcagaggcc ttaggctatg atttgtggga tttggttcaa aatgggccag agacaaagct
     6721 aaaccagact gaatacacgc aggctgccat gttaactgct gatgttgcaa tttatcgctt
     6781 attaatgcaa ttaggcgtac ctcagcctca agtaatggct ggccatagct taggggaata
     6841 cgctgctttg gtttgcgcta attctttatc tttaaaggat gccgcacagt tagttgctcg
     6901 tagaggacag gtgatgcaaa acgctattcc tctgggagag ggggctatgg ctgccatagt
     6961 aggcttatct gatgaacagg ttcgttcatt gtgtgagcaa gccagcacag ccaatcatct
     7021 tgttaccccg gcgaattata atgccctggg ccaaattgtt gttgctggac atcgagtggc
     7081 cgttgataaa ctaatacaat tggctgaaga gactggcgct agactggcaa tgataattcc
     7141 tgtaagtgtg ccttgtcatt gccctttatt aaaaaatgct gcggaattat ttgctgaaaa
     7201 tcttgctcaa attgaatttc aaattccttc agtcgatgtg ataagcaatg ttgatttaag
     7261 tatttatcat tccacccaac atataagaga taaactcaaa gagcaactat acagccctgt
     7321 gcgttgggtt gaaacagttc agttaattca acagcgtgga attgaactgg ttgtggagtg
     7381 tggaccagga aaagttctga acggtttgat aaaaagaatt gagagaaatt taactacgat
     7441 tagcgtttat gacaccatta gtcttgatca agtcatagag cgtttacata tcccagtttg
     7501 agaggaaact agatgatcaa tttagaaggt aaaattgcat tagttactgg tgccagccgt
     7561 ggcataggtc gtgctgttgc cgagaaattg gcaagaaatg gtgcctttgt ttttggaaca
     7621 gcaactactg aacagggtgc tgcgtctatt aattcttttt ttgagaaaga aaaattggct
     7681 ggtaaaggaa tcgttttgga tgtaaccagc actgagcaag tagaaaattt gatgtctacc
     7741 ttatctgata atgatcagtg tccttctatt cttgttaata atgctggcat taccgcagat
     7801 aacctgttat tgcgtatgga tgatgaggaa tggtacaaag taatagaaac aaatttgaat
     7861 tctatttaca gaatgactaa agcttgctta aaacctatgt tcagagcacg ttggggaaga
     7921 ataatttcta taggctctgt ggtaggtgcc agtggcaatt ctggacaggt aaactatact
     7981 gcagccaagg ctggagttat tggtttttcc aagtctttgg cacaggaaat tggtagtaga
     8041 ggaataactg taaacgtggt agcccccggg tttatcgaca cagatatgac tactgcattg
     8101 cctgatatgg tgaaagagga aatgttaaaa agaattccca tgcgtaagct gggccagcca
     8161 gaagatgttg ctgatgctgt agcttttcta gcatcagaca gcgcaaaata tatcacaggt
     8221 gaaacaattc atgtcaatgg cggtatgtat atgagttaaa tgcacttgcg ttatctgtat
     8281 aattgaataa actagccgtc agtatttaat aatttctatc aaccgaaaga ggaaaattac
     8341 gttatgagta cagttgaaga gcgtgttcgc aaaattgttg ttgaacaact gggtgttaaa
     8401 gaagaagaat taaagaatga tgcatccttt gttgatgatt tgggtgctga ttccctggat
     8461 acagtagagt tggttatggc tcttgaagaa gaatttgaaa cagaaattcc tgatgaaaaa
     8521 gctgagaaaa ttactacaat tcaagaagca attgattata ttgaatccaa cttaaataaa
     8581 gaagaagctt aattttttta aggagctctc attgaatagt cggcgtgtgg ttattaccgg
     8641 catgggtatg cttactcctg tcggacttaa tgtagaagaa acttggcgaa gtattttagc
     8701 tggggttagt ggggtaggtt tggtagaaga ttttgacact actgaatacc caactaagat
     8761 ttgggctaag gtcaagaatt ttaatgttga gaatcacatg ccaattaaag acgcccgcaa
     8821 aatggacgtc tttacacaat acggtatggc tgctgccgat gaagcgatgc ttgactcggg
     8881 tttaaaaatt gatgaaacct tatctcgtcg aatcggtgtc gcagtggggg caggtattgg
     8941 tggtattcaa acaatcacaa ataaccagga taaactggtt gccggtggac cacgtaaggt
     9001 ttcgccattt tttattccgg caggtattat caatatggtt gctggacaga tttctattaa
     9061 acataattta aaaggaccta atatttcggt tgtgactgca tgtactaccg gaacacataa
     9121 tatcgggcta gcagggcgca taattgctta tggcgatgcc gatgttatga tttgtggtgg
     9181 agcggaaatg accacaacgc cactttgtct tgctgggttt tcagcagtaa gatctttgtc
     9241 caagcggaat gatgagcctg agaaagcctc aaggccttgg gataaagaca gagacggttt
     9301 tgttatgggt gagggtgctg gtattttggt tttagaagaa tatgaacacg ctaaagctcg
     9361 tggagccaaa atttatgctg aattggttgg ttttggtatg tcaggagatg cttatcatat
     9421 tactgcaccc gatgaagatg ccgatggagc ttcacgagct atggaggcag ccatccagga
     9481 tgctggtatc gatcccagac aagttgatta tattaatgct cacgggacgt cgacttattt
     9541 aaacgattta aatgaaacaa aagccattaa gcgtgttttc cagaatcatg cttatgatct
     9601 ggcggttagc tcaaccaaat ccatgacagg acatttgctc ggagctgcag gtgcagtaga
     9661 ggcaattata agtattttag ccataagaga tcaggtggct ccacctacaa taaatttgga
     9721 taatcctgat gaaggttgtg atttgaatta tgtagcacat gtaccacaaa gcagaacaat
     9781 taattatgtt ctaagtaatt cacttggttt tggtgggaca aacggtagct tactgttcaa
     9841 gcgaatctaa atgcaattct ctagacataa aaaactggtt attcttgttt tatccttgtt
     9901 ttttatgtct ttcttgtttt tattttggaa tatatatcga ttattgtatt caccaataat
     9961 tccgaaaact gcttctccaa tgattattgc cttggataag gcgacgtccg cttatcaatt
    10021 tgcttatatt ttgcaagata aaaaattaat acattctgcc aaattttttc tattgattat
    10081 tcgttttgaa ggattatcgc atcagttaaa ggcaggcgtg tatcaaatta ctcctggcga
    10141 aactgcaatg aaattactac atcgagtagt tgctggtgat gtaattacgc aaaattttac
    10201 gatcatcgaa ggatctacac agcagaaagt agattatgac ctaagacaag ctaattattt
    10261 gaaatacaat ccagaagatt gggcaatagt aaaagaaaat tacccaagtg cagaggggtt
    10321 acttctagca gacacttatc aataccaagg tgggagtagt agtaggacat tattagagca
    10381 agcccatcgt aatttattaa attatcttaa taccagttgg acaaacagag caccaaattt
    10441 gccttataaa actgcttatg agctattgat tgccgcgtca attattgaaa aagaaactgc
    10501 aattgcacaa gagaaaaaat tgatttctgg tgtgatggta aacagattaa agaaaaaaat
    10561 gccattacaa atggatccta cggttatcta tgggctggga aatcagtata caggaaaatt
    10621 aacacataat gatttgctaa ttcaatcacc atataattct tatttgaaca gaggattgcc
    10681 tcccacccca atagctatgg ttggtaaaga ggccatagat gctgctgcac atcctcaatt
    10741 gtccaattat ttatactttg ttgccaaagg agatggcact catcaattct ccgaaactta
    10801 tgagcaacaa agacaagcga ttaatcaata cagacgcaag gattattaat gtcatcgtta
    10861 accgggaaat tgatagttat tgaaggtttg gagggggcag gaaaatctac agctgtcaat
    10921 ttagttgttg aactgttatc ccaaaaaaaa atcagtacta taaccacgag agaacccgga
    10981 ggaacaagaa taggtgaaat tttaagaagt attattaaaa atccagagta caataatgtt
    11041 ctggatgaca gaagcgaatt acttctcctg tatgccgcaa ggatacaact gattgagcaa
    11101 gtgatcaaac ccgctttaaa tgtggggcag tgggtcatag ctgatcgatt tgagctttcc
    11161 actttagctt atcagggagg gggccgcaaa atgaatatgc gggttattaa tgagctatcg
    11221 aatttctgtt taaaaggatt caaacctgat cttactttat atttagacat taatcccgag
    11281 cttggcatga taagagctaa atcaagggga aaatttgaca ggattgagca ggagtcaatt
    11341 gagttttttc atagaataca tgatacatat catgttcttg ttaaacaaaa cccggaaatt
    11401 atgatgattg atgctaacag gtctttggat gaggttcaat cttccattca atcagtaatc
    11461 gaggagttta tagagcataa cctatgatta cttatcaaga acaatggcag aaaatgcaaa
    11521 tggccttggc ggtaagacga attcctcaag ccatgctatt tttgggttct ttgcattgtg
    11581 gtcttataga atttacaaat caagtgatca aattactctt gtgtaaaata aaacagaatg
    11641 agccttgttt gacttgcatg gattgccaaa tgatagagcg ttttgagcat ccggatgtgg
    11701 aatggattaa gcctgaaaga agcggtggta ttattaaaat tgatcaaatt aggggattgc
    11761 agagcacggc gtttctaaca ccacagagga ctaatcataa acttataatt atcgaatcag
    11821 ctgaatcaat gaatattgcg gcatcaaatg ccttattgaa aattctggaa gagccagcgc
    11881 aacatactat ctttatttta ttagcgcaac aaattggcaa tataccaccc accgtattga
    11941 gccgatgcca aatgacgcaa tttgctgttt ttgatgagtt gccttacaac aatttattgg
    12001 ctttaggaga gtactatact caggaatcga atagcacaat ggtcttcaag cattcagaat
    12061 ccattttaga aggattgatt gcaattattg agcgaagaga gcatccatgc actttggctg
    12121 cacaatggat tcaacatgaa ttaggtgctt ttttgtggtt tttatatttg ctctattctc
    12181 aactacaata tatgcatttg tttcgagtgg cttattctgg tacgttaaaa gaacaattga
    12241 ataagctatc ctcattactc aatccggtta tgatttttgc ccaagtagat aaaatcaata
    12301 ctttgttaag aaaattaagc cataatatga atatgaatca tactttggtt ttggaagatt
    12361 tattattttc tttggttgtt gattattaat tgagtttaaa ggaattaaac cattgcgtgt
    12421 gaaccgagtt tttattaagc aactgaagta agcaagataa ttagttaaaa caggactatt
    12481 caaggaggat gtctattgat tgagcggagg agtcatgacc gaaaaaccac agcttataaa
    12541 ctgctcattt cccaatgaag catctttgta catggcttac atgccttttg tgaagggtgg
    12601 cggtcttttt ataagaactc aacacgttta ttctctaggg cagtcgcttg aattatctgt
    12661 taaattgctt aatgaaaatg aaccatattt ggtcactggt aaaatagtct ggattactcc
    12721 taaaggtgca caaggtaata agccgccggg agttggtatt caatttaccg gtgaaaacag
    12781 ccgttatcta tgtaacaaga tagaaactta tttggcagga atgctaaaat catgtcaggt
    12841 tacagatact atctagaatt atcatttatt atataatgag caatattttc attgatatca
    12901 ttttggggta taaaggtgct ggtcgactct cattgtcatt taaattttat cgatttgacg
    12961 cattttaatc atgatcttgc tcaggtcatc aatcaagctc gcgagaatgg ggtggaacat
    13021 ttcttgtctg tttgtgttga gttgagcgat tatcctcaat tagagcgttt agctaaggat
    13081 tatcctgata ttagtatttc tgttggagtg catccaaata gcgaaatgaa ttatcctgtt
    13141 acggcacaga tgttatgtga attggctcaa aatcctgcct gtattgccat aggagaaaca
    13201 gggcttgatt attatcgtaa tcccactgag gaagctcaat ctttgcaacg agatcgcttc
    13261 agggaacata tcaaggcggc gcttataact tccaagccgt taattattca taccaggcaa
    13321 gctgctaaag atactttgtt gatgatggct aaggaagggg ctagtcagat aggcggggtc
    13381 atgcactgct ttgctgaaga cttggatatt gcgcaaagag caattgattt aaatttttat
    13441 atatcatttt ccgggatagt tacttttaaa aatgctgttt cattgcaaga tgtagccagg
    13501 cagatacctt tagagaagat attgatagag acagattcac cttatttggc tccggttcca
    13561 cacagaggaa agcaaaatca tcccgctttg gtgaaacacg tagcagaaac tatcgcacaa
    13621 ctcaga
//
LOCUS       DAXLDT010000080        13530 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXLDT010000080 DAXLDT010000000
VERSION     DAXLDT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.080"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(455..901)
                     /locus_tag="ACF2SP_001885"
     CDS             complement(455..901)
                     /locus_tag="ACF2SP_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFD2359668.1"
                     /translation="MKHNLLDKAYKHCVNHGYRFTEPRERVLKILVDERKPLGAYDIL
                     QRLSMEVDNPKPPTVYRAIQFWHQEGFIHCIDSLKSYVACLHGHHVGQAQFLICNQCD
                     FVKELECVVDFTSVTEAANSIQFSIINCTVEIKGLCSDCNLTNLKN"
     gene            1233..3791
                     /locus_tag="ACF2SP_001886"
     CDS             1233..3791
                     /locus_tag="ACF2SP_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD2359669.1"
                     /translation="MLYSNYRFYLAKNAQLLNPEESREAIEKILAKTKELELIRVAVD
                     LTSSNKSISLLSASKESHVIKTKVTQILKEQGLSVIDMMEVEVLQDIEKIEHHHPTHK
                     SKNTPLHPIKKHQKKAKHDHSHGHGHHHGHHHENHWIKAALGLIWGIGLLVLSIGTFN
                     IPLVAYYVITGLTSLMTLYLGHTVYQSAWQALKEKKWDTTTLYSISTLTILAVSIASV
                     FVPGLPMMFEAAPLVLGFWHLGEGIEHTLVGKINKKLDVRDCVPPLVLLKGNPDKEIS
                     VKTLIPNDMIIVKKGQVIPVDGVLTQQALLYTTRIDGSPNLKEFHPGDVVKAGMQLAD
                     HIPSLAMQVTKTYQNSYLSLIAENINKANDEKAPIEMFADKVLKYFIPGLIAVALLSG
                     VVIGSLFNPALAIQCVVSVLVSACPCALSLITPMAVKIGMKKASENGIHFNNGKALQA
                     AADIDTVVFDLNGTLTQGKMVVKSLHITDKKYLRPIALLESQSDHPVAKIIKSYIEQQ
                     DTASTEPLEITSIDKSHHSGMKGIVNGEAFMIGNKDMLAANGITSIDKPYDNPEKGSV
                     YIVRGTTVIGQIALIDPLREDAIATVKQLKRLGKSVHICTGADQATAEKYATLLGIPK
                     DNICANTVGAVTQKGEVSKTSYIQQLKQKGFKVAMVGDAANDATAIAYSDIGIAVKSS
                     IGDSITEQHAGIVVQQGLLFPIATAFDVAQKTKQNIFQNLFVSLSYNSIITLVAAGLF
                     VALGFALNPVLGVALMVLESTIVLTNLYLFKQQDILSSTSSNANNAVEVEPTANTTSK
                     VLHALGHRSQPQENLAIVTESKPVNPKVINLFAPKEHSSYRSDVTQQKLGCGIC"
     gene            3937..6067
                     /locus_tag="ACF2SP_001887"
                     /pseudo
     CDS             3937..6067
                     /locus_tag="ACF2SP_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            6310..6492
                     /locus_tag="ACF2SP_001888"
     CDS             6310..6492
                     /locus_tag="ACF2SP_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359670.1"
                     /translation="MNEAGYRKKISAVSKEYLQAPGLRLAKNTMFIQDVDWNHGIVIR
                     ACFKKRRIISDSVTLV"
     gene            complement(6587..6925)
                     /gene="cyoD"
                     /locus_tag="ACF2SP_001889"
     CDS             complement(6587..6925)
                     /gene="cyoD"
                     /locus_tag="ACF2SP_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFD2359671.1"
                     /translation="MEHHGIKEPDYGTGQKKLGLYLVGFISCTFLTLFSFWVVMSHIF
                     SKTEVLTLIFSSACIQFLVQVICFLRLSTQTQQGRTNVMTFLFTGVILTSIILGSLWI
                     MWNLNYYMVH"
     gene            complement(6927..7523)
                     /gene="cyoC"
                     /locus_tag="ACF2SP_001890"
     CDS             complement(6927..7523)
                     /gene="cyoC"
                     /locus_tag="ACF2SP_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFD2359672.1"
                     /translation="MHNETILNHIHHHDSDSTDVFGFWMYILTDCILFGCLFATFLVL
                     NNPGYPGPALKNYVNLHDVLIETFLLLISNFTFCLAILNLYQDKRHKVQLWLGITFLL
                     GAGFISMEVTEFIHMANEGFRWDVSGAISSFFTLVGTHGLHVGFGLLWILIIIIQLPL
                     IKLNHVKRRRMTYLGLFWNFLDIVWIFVFTIVYLMGAI"
     gene            complement(7523..9517)
                     /gene="cyoB"
                     /locus_tag="ACF2SP_001891"
     CDS             complement(7523..9517)
                     /gene="cyoB"
                     /locus_tag="ACF2SP_001891"
                     /EC_number="7.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFD2359673.1"
                     /translation="MLQNLLFGKLTLDAIPFHNFFAMGAGIFMVLALGGVLALLTIFK
                     KWSYIWNEWVTSVDHKKIGIMYLILAFVMLLRGFSDALLMRSQQAVAAGNAIGFLPPD
                     HYDQIFTAHGVIMIFFVAMPLVFALLNMIVPLQIGARDVAYPYLNSLSFWLTFVGAML
                     CNISLVVGQFASTGWLAYPPLSELYYSPDVGVDYYIWSLQISGVGTLLAGINFIATIL
                     KMRCNGMTLMKMPVFVWSALCAMILVALAFPVLTVTLALLSLDRMLDMHFFSDFAGGN
                     SMMYINLIWVWGHPEVYILILPVFGIFSEVVATFSQKKLFGYTTMVWAIAMITLLSFL
                     VWLHHFFTMGAGADVNSFFGIATMIIAIPTGVKVFNWLFTMYKGRIIMATPMLWVTAF
                     FTTFAIGGMTGVMMAVPPVDFQTHNSLFLVAHFHNVIIGGVLFGAFAGITYWFPKAFG
                     FRLDETWGKRAFYFWVTGFYCAFMPLYVLGLDGFVRRTNHYANTMYQPYLVFAAMGAL
                     LILVGIVFQVIQLIVSIKNRHALHDRTGDPWNGRTLEWSVASPAPIYNFAKLPTVQAL
                     DQFWVDKEHHRAEGHIHPKDIHYDPIHMPKNTSTGFIIAIFSGIAGFALIWHMLIPGI
                     IGLLGVVSTAIARTFSNDVDYYIDAETVKKTELAHFRELV"
     gene            complement(9520..10467)
                     /gene="cyoA"
                     /locus_tag="ACF2SP_001892"
     CDS             complement(9520..10467)
                     /gene="cyoA"
                     /locus_tag="ACF2SP_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HFD2359674.1"
                     /translation="MVARTRTTTININAFRWLLKVVFIISGSLILLSLSGCKEHFIGM
                     LNPKGLIAFEQRKLFFDTLALMLIVVLPVIIMSITFVYHYQVSHRTRDYKPNWGHNFY
                     LEALWWGIPCVIILVLGIVTWKKTHELDPYRPIAGQAQKPLLVQVIALPWKWLFIYPE
                     QNIATVNYLEIPLGQQVEYWITTDNVPMSAFFIPQLGSQIYAMAGMRTRLHLLASEPG
                     VYDGLNTQYNGDGFSDMHFEVHVVEPKTLQQWFTEVKKSPNGLTDASYNQLLYPSIAD
                     KPKLFSSVQNDLFNKVIMIYMESIGRTHPRENIVKFHKE"
     gene            10576..10968
                     /locus_tag="ACF2SP_001893"
     CDS             10576..10968
                     /locus_tag="ACF2SP_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359675.1"
                     /translation="MTYIIFARWFGLVSVILSLGILFNLEDARDMAKHMVKSDSGYIM
                     GGVLPTVFGSLSLMFANSFELGWTLIITLIGASMLLIGIFRVIFVKQWKALLSQYIQE
                     IPFLFSLFGLMFGVILLYVGFIANSFAF"
     gene            complement(10943..12238)
                     /locus_tag="ACF2SP_001894"
     CDS             complement(10943..12238)
                     /locus_tag="ACF2SP_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359676.1"
                     /translation="MIKKEILISSLGNTIEWFDFGLFIFMAPIIGAKFFPQASAGNAT
                     IEAFIVFAAGFLCRPLGGIFFGYFGDTRGRAKTLRISILIITFSTFLLGVIPSYEEIG
                     LTAPIIFILLRLIQGVSIGGEYSGIIIYLAESAPLNKRGFTTSFAALGANLGFLLATV
                     TLLFLHLFFSEETITNWAWRLPFLFIGLPGSLIIYYRFKLSETPVYSLLQKKHRLVSI
                     PFIVAIKFAPRQLLKILGLTCMGSTFYYVFFAYIPNYLEHYIGFSLANALTIESFMLI
                     IMLFTVPLAGLCGDFFTRKKMLIITAIAILFLVLPCFYLLQFKSIILALLSLGTATIL
                     SSLEQGNTLAAIVENCPENIRYSGIAFSYNLGNALFGGSTPLIVTLLTENKGLITPAY
                     YLIFMAAITLITATTLLSNNQSLGPLRNPMRSRTQMNLQ"
     gene            complement(12331..13029)
                     /locus_tag="ACF2SP_001895"
     CDS             complement(12331..13029)
                     /locus_tag="ACF2SP_001895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2359677.1"
                     /translation="MANYLVIAASSAIGQAVVNSLISQGDTVFTTARDNSKIQPNVLL
                     DATDFEAVNEVFKQFGPIDGVVNCAGSLLLKSAHTTAKEQYQAVIDSSLTTAFATIRA
                     AGMHMKGGGSVVLISSAAALVGLANHEAIAAAKSGIIGLAQSAASTYADLNLRFNVVA
                     PGMTDTPLTAQLISNTLAFKASKAMHALDRIGTPEDIARTIIFLLDPKNNWITGQVLT
                     VDGGLSRVRPKMKI"
     gene            13141..13401
                     /locus_tag="ACF2SP_001896"
     CDS             13141..13401
                     /locus_tag="ACF2SP_001896"
                     /inference="COORDINATES: protein motif:HMM:NF026153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFD2359678.1"
                     /translation="MTKERDSNTDFCSQCGHQSIVFSTPPNDTRVRKVCNYCLTVHYQ
                     NPKIAFGTVTMFKDKFYYATVRLNCSWVYGFILLVFQKMVNL"
BASE COUNT         4492 a         2648 c         2565 g         3825 t
ORIGIN      
        1 cttatcaacc gaataccatg ttttttttca tagtaattaa aaatctcgcc taaatcttcg
       61 gcagtaagca ttgttgttgt atcatacaac cctgtaccaa caggataagc ttggaatcgt
      121 tccttacgaa cagatttgct ttgcataaca gtaaccaaaa gctcatactg attaataact
      181 agtctagtag gttgttttat ttctgtcaaa cgatggatta agacaccaat ttattcgact
      241 acatgtgaaa aaatgtattt tatcaagtac taaacatccc agatagaaat gccaatcatt
      301 aattgtaatg atgaaaaatc atatgaacaa aaattagcat gaagatggat atacacaaac
      361 ggcaatgaag ctaatcaaaa caatattatt ttgctcgaca aggagtaaat gaatataatt
      421 ttttacaaag cattaaagga gttattgcca gaaattaatt tttcaggtta gtcagattgc
      481 aatcagaaca caatcctttg atttctaccg tacaattaat aattgaaaat tgaatggaat
      541 tcgccgcttc tgttaccgaa gtaaaatcaa ctacacattc caattctttg acaaaatcac
      601 actgattgca tataagaaat tgggcttgtc ccacatgatg cccatgcaaa caggcgacat
      661 aggatttcag actgtcaatg caatgaataa atccctcttg atgccaaaat tgaatagccc
      721 ggtagaccgt tggtggcttg gggttatcca cttccataga taatctttgc agtatgtcat
      781 aagcaccgag tggctttctc tcatcgacta atattttgag gacacgctcc cttggctcgg
      841 taaatcgata gccatggttt acacaatgtt tataagcctt atccaacaaa ttatgtttca
      901 ttaatcttct ttcaagcgaa tagttagtgc gcactatttt atcataatcc ctgttgcaag
      961 gaaaaatgat caaacaacag ccaatcatta tttcatagaa atcatttact gtctaaaaat
     1021 tatttaattt ttcttgttat ctaacaatta aatgttacaa tataacattt tttgagaaag
     1081 gcagtatgtg ctcgtaacgc ctggcttagg aagaaatctt attagggaca aacagcggga
     1141 tctattgtgc agatcctgtt tcaaaatagg catttgcaga aaattaacct ttctctatag
     1201 caaccagaaa cccctttaaa tagaggaaag ccatgctgta tagtaattat cgattctacc
     1261 ttgcaaaaaa tgcccaactt ctcaacccgg aagaatccag agaagcaatt gagaagattt
     1321 tggctaaaac taaagagttg gagttaataa gagtagctgt cgatttgact agttcaaata
     1381 aaagcatcag cttattgagc gcatccaaag agagtcatgt cataaaaacc aaagtgaccc
     1441 agattttaaa ggagcagggt ctttctgtaa tagacatgat ggaagtggaa gtactccagg
     1501 atattgaaaa aatagagcat caccatccta ctcataagtc caagaacaca cctcttcatc
     1561 ccattaaaaa gcaccaaaaa aaagcaaaac atgaccattc tcatgggcat gggcaccacc
     1621 atggacatca tcatgaaaat cattggatca aagcagcatt aggtttgatt tggggaattg
     1681 gattacttgt tctttctatt gggactttta atattcccct ggttgcttac tatgtcatta
     1741 caggacttac ctctttgatg actttgtatt tggggcatac agtttatcaa tcagcctggc
     1801 aagcattgaa agaaaaaaaa tgggacacca ctactttata ctcaattagc actctaacta
     1861 ttcttgctgt ttctattgcg agcgtttttg tccctggcct acccatgatg tttgaggcag
     1921 cccctctggt tttaggcttt tggcatttag gtgaaggaat tgaacatact ctggtcggta
     1981 aaatcaataa aaaattggat gtccgggatt gtgtaccacc attagtcctg ttaaaaggta
     2041 atccggataa agagatttca gtcaagacat taattcctaa cgacatgatt atcgttaaaa
     2101 aaggacaggt cattcctgtt gatggtgtct taacgcaaca agcgctcctt tataccacaa
     2161 gaattgatgg ctcgcccaac ctcaaagaat ttcaccctgg ggatgttgta aaagccggca
     2221 tgcaattggc agatcatatt ccttctctgg caatgcaggt taccaaaact tatcaaaatt
     2281 cttatttgtc attgattgcc gaaaacatta ataaagcgaa tgatgagaaa gcgcctatcg
     2341 aaatgtttgc agacaaggta ttgaaatatt ttattcctgg tttgattgcg gtagcgctcc
     2401 tatcgggagt tgtgatcggt tcgttgttta acccggcact ggctattcaa tgcgtcgtgt
     2461 ctgttctggt aagtgcctgc ccgtgcgctt tgagtttaat taccccaatg gcagttaaaa
     2521 tcggtatgaa aaaagcctct gaaaacggca tccactttaa caacggtaaa gccttgcaag
     2581 cagcagccga cattgacacc gttgtttttg atttaaatgg cactctgact cagggcaaaa
     2641 tggtggtgaa atcattgcat attaccgata aaaagtattt gcgccctatt gcattactgg
     2701 aaagccagtc ggatcatccg gtagcgaaaa ttattaaatc ttacatagaa cagcaagaca
     2761 cggcttcaac cgaaccatta gaaataacgt caattgataa gagtcatcat tccggcatga
     2821 aaggtattgt taatggcgag gcctttatga tagggaataa agacatgctt gcagccaatg
     2881 gaattacctc tatcgacaaa ccctatgata atcctgaaaa aggttctgtt tatattgtgc
     2941 gcgggacaac ggtgataggt caaattgcct taattgatcc attgcgagag gatgccattg
     3001 ccacagtgaa acaattgaaa cgtcttggta aatccgtaca tatttgcacc ggggcagatc
     3061 aggctactgc agaaaaatat gcgacattat taggcattcc caaagataat atctgtgcga
     3121 ataccgtggg ggctgtcacg caaaaggggg aagtatccaa aaccagttat attcaacaat
     3181 taaagcaaaa aggctttaaa gtagctatgg ttggtgatgc cgctaatgac gctaccgcaa
     3241 ttgcctattc cgatataggc attgctgtta aatcaagtat cggcgactcc ataactgaac
     3301 agcatgccgg catagtcgta caacaaggtt tattatttcc tatagcaaca gcatttgatg
     3361 tagcccaaaa aacaaaacaa aatattttcc aaaacctgtt cgtgagccta agctataact
     3421 caataattac gcttgtggca gcggggttgt ttgtggcatt aggttttgca ctaaatcctg
     3481 tgctgggtgt cgcattgatg gtgcttgaat ccactattgt tttgacgaac ttataccttt
     3541 tcaagcaaca agacattttg tcctcaacat catccaatgc caataatgct gtagaggtcg
     3601 agcccactgc caatacaacc tcaaaggtct tgcatgcttt agggcatcgt tcccagcctc
     3661 aagaaaattt agcaattgtg acagagtcta aaccagtcaa tcccaaagta ataaatttgt
     3721 ttgcacctaa agaacattct tcttataggt ctgatgtcac tcagcaaaaa ttggggtgtg
     3781 gaatctgttg atcaagcggt actgaaattg ccttgatttt ctacttcaag gcaattgtta
     3841 cggtagtcat cttttcataa ataactgttt tttggactat actcttatca agaccatgca
     3901 aagcatcaag ccataaaatt aggatgattc aatgccatga ataacacttg gcatcaaacc
     3961 gccactggaa accgccctat tttaagtaaa ttgcgattaa gcagaactct acttaacgtc
     4021 atctttatta ttgccctttc tttgctttta atcatcaata ttatttctta taaccaggtc
     4081 aaaaatttaa tcttcgccag taactgggtt tctcatactc atgaagtcat ccaaaccata
     4141 gatacatgcc tttatgatgt agtagaaatt gaatctcgcc agaggtttta cttgattcga
     4201 ggttcaagcc ctcagcttat gacgggatat tgatgatatg aaatcaacat tacaactgaa
     4261 tttatacaaa cttgagcaat taaccaggga caacccagaa caaaacaaac gaacccatcg
     4321 ttttattgat ttggttaagc aaagattaaa tttgttaaac caacttgtac agttaaaggg
     4381 aagcggcaaa ctcgatacac aagaaggtta tgatttactt aaccaaagtc aagacatgtc
     4441 gcatcaagtt aaaagtcttg gacaagaaat caaatccgtt gaattggtgt tgctatcaga
     4501 aagaaatact ggtgcggttc gctgggcaga taccagtagt tttattttaa tatccggagg
     4561 tattacaagc atcctgtttt taacgatcgc ttttattctg gcaaatattg aattgtcaac
     4621 acgcagaaaa actgaactac agaatcaaaa tacgcaaata cggttgaaga aaattataga
     4681 aagtgccagt gatatgatag cggcttttga caaagaccag cgatttatca cttttaatga
     4741 agcttatcaa cgtgagttta aacgtctttt tgataaatcc ataagcatca atatgtctct
     4801 ggaagaagct ttcgatgatg ttcctgaaaa caaaaaaaaa ttggtgcaaa cctggaaaga
     4861 atctctacaa agggatgaag aaaccaaaaa tatcgaagtg aatacagaac aggaaaagat
     4921 tatttatgaa atgacctcca aactcatcca aaacggtgac aatgaaatta aaggtgtggt
     4981 acacagcgtt cgcaacatta ccaaacgggt acaagaacat actgaactac aagagtcata
     5041 tgaaaaactg gctaatggaa tgaaagaact gcaaaagaaa aatgaacaaa ttacccttct
     5101 ggttgagatg agcgacatca tgctggcttg cggctcccag gaagagctaa gctacgtaat
     5161 ggcgaaatac tcccaacgac tactgcaatt ttcaagcggc tacctgttta tcatgcatcc
     5221 ttccaaaaat tatttggaaa aagcagccag ttggggtaat cctcaaccgc atgatctgac
     5281 ttttaccccg gaacaatgtt gggctatccg gcttggccga attcattatg ccggttcatc
     5341 gcgtatcgag ttgatgtgta gtcatacgat gtttgccgag caacccgagt tgtctctgct
     5401 gtgtgtaccc ctaatggctc aaaatgacat ttacggattg ttataccttg aagttggcct
     5461 agaatttgat gaaaaccaac aattactaat aaccgcattc gcagagctta ccgccttggc
     5521 tttggcaaat gtccgcctga gagaaaattt acgctatcaa tctatccgtg atccactcac
     5581 tggattatac aatcgccgtt atctggaaga ttttctattt aaacaattac atcaggctga
     5641 acgaacaaaa gcctcatttg ccattttaat gctggatttg gatcatttta aaaagatcaa
     5701 cgataccttt ggtcatgatg caggagatct tgtattaaaa gagctggggc aaattttaaa
     5761 cagtgatatt cgcctcggtg atattgcagc ccgatatggg ggagaagaat ttgtcctgct
     5821 gctctatgat attgatgctc aagccgctaa aatgaaagcc gaaaatttgc gttcggccat
     5881 ttccaattta caggttaaat acggagcgca acctgttggg cagataacgg cctcaatcgg
     5941 aatatcggtt tatcctgacg atgccaaatc acctgctgaa gtgattgaag cagctgataa
     6001 ggctttatac caagccaaaa acaaaggcag gaataaggta attttattct cggaaatatc
     6061 atcctgattt ggattgcggc tcctgaccta aatgttgtga tgtttcttat gttcgataga
     6121 gaggttcatg ctttatatga gttttgagag cgaaacaata gatactacac tctatagcat
     6181 ccggtatttt gtatccgttc gcgcagatac acctaacaat tcccaaaaaa tgcatagact
     6241 gagctgctca aaaagagggg ctaaagatga aatcaaaaga agaacattga gagcgtttat
     6301 ttaaaggata tgaacgaagc gggttatcgc aagaagattt ctgcagtgag caaggaatac
     6361 ctgcaggcac caggcttgcg cttagccaaa aacactatgt tcatccaaga tgtagactgg
     6421 aatcacggta tcgtgattcg tgcatgcttc aaaaagcgca ggattatcag cgatagcgta
     6481 actcttgttt aaaccaaact aaagaggtac tcatgtcatc tttttttatt tttttgtgga
     6541 aggtgatttg ctctgtgagt tgaagggaaa ttgagagcaa tagcttttaa tgcaccatat
     6601 aataatttaa attccacata atccataagg atccgagaat aatagatgtt aaaatgacgc
     6661 cagtgaatag aaaagtcatc acattcgttc ttccttgttg tgtttgtgtg ctcaagcgaa
     6721 ggaagcaaat gacctgtacc aaaaattgaa tgcatgctga agaaaaaata agtgtcaata
     6781 cttccgtttt agaaaagatg tgcgacatga ctacccaaaa tgagaacagc gtcaggaaag
     6841 tgcaacttat aaaaccaact aaatacagcc ctagtttttt ttggcctgtg ccataatccg
     6901 gctcctttat tccatgatgt tccatgttaa atagccccca tcaggtatac aatagtaaat
     6961 acaaaaatcc aaacaatgtc taaaaaattc caaaataacc ccaagtaggt catacggcgt
     7021 cgcttcacat gatttaattt aatcaaggga agctggataa tgattatcaa aatccacaac
     7081 aaaccaaagc ctacatgcag tccatgagta cctactaaag tgaaaaacga ggaaattgca
     7141 ccactcacat cccatcgaaa accttcattt gccatatgta taaattcagt aacttccatt
     7201 gaaataaaac cagcaccaag caagaaggta ataccaagcc atagctgaac tttgtgtcgt
     7261 ttatcctggt ataaatttaa aatagccaag caaaaagtaa agttagatat cagcaaaaga
     7321 aatgtttcga tcagtacatc atgtaaatta acataatttt taagcgcagg ccctgggtat
     7381 cctggattgt ttaatactaa aaaagttgca aataaacaac caaatagaat gcaatctgtc
     7441 agtatataca tccaaaaccc aaatacatct gttgaatcgc tatcatgatg atgtatgtga
     7501 ttgaggatgg tttcattatg cattacacaa gctccctaaa gtgagctaat tcagtttttt
     7561 tgactgtttc agcatcgata taataatcaa cgtcgtttga aaacgttcga gcaatagctg
     7621 tagagacaac tcccagtaaa ccaataattc ccgggataag catgtgccaa attaaagcaa
     7681 accccgcaat tccggaaaaa atggcaataa taaatcctgt cgatgtattt tttggcatat
     7741 gaattgggtc gtaatgaatg tctttaggat gaatgtggcc ttctgcccga tgatgctcct
     7801 tatctaccca aaactggtcg agtgcttgta ctgtcggtaa tttagcaaaa ttatatatgg
     7861 gtgcaggcga tgcaaccgac cactccaatg tccttccatt ccatggatca cctgtacggt
     7921 catgaagcgc gtgacgattt ttaatgctta caatgagctg aataacctga aatacaatgc
     7981 ctactaaaat taacaaggca cccatcgcgg caaaaactaa atagggttga tacattgtgt
     8041 ttgcgtaatg attagtgcga cgcacaaatc catcaaggcc taaaacgtac aagggcatga
     8101 aggcgcaata aaagccggtc acccaaaaat aaaaagcgcg tttaccccaa gtctcatcta
     8161 aacgaaaacc gaaagctttc ggaaaccaat aggtgattcc agcaaacgcg ccaaatagca
     8221 caccaccgat tatcacatta tgaaagtgtg caacgagaaa cagactgtta tgggtctgaa
     8281 aatcaactgg ggggacggcc atcatcacac cggtcatacc acctattgca aaagtagtaa
     8341 aaaaagccgt gacccaaagc atgggggttg ccatgataat tcggccttta tacatagtaa
     8401 acaaccagtt aaatactttt acccccgtag gaattgcaat aatcatagtc gcgattccaa
     8461 aaaaggagtt cacatctgct ccggcgccca tagtaaaaaa atggtgaagc cacaccagga
     8521 aagataaaag cgttatcatt gctatggccc ataccatagt ggtgtaacca aataattttt
     8581 tttgactgaa cgttgccacc acttcagaaa aaataccgaa cactggcaaa attaagatgt
     8641 atacctccgg gtgtccccat acccagatca aattaatata catcatgctg tttccaccgg
     8701 cgaaatcaga aaagaaatgc atatcaagca tgcgatctag cgataaaaga gcaagcgtta
     8761 ccgttaaaac tggaaaagca agagctacta aaatcatggc gcacaaagca gaccaaacaa
     8821 aaacgggcat tttcattaaa gtcatgccgt tgcaacgcat ttttaaaata gtcgcaataa
     8881 aattaattcc tgcaagcaag gttccaacgc ctgaaatttg caaggaccag atatagtaat
     8941 ccacacccac gtcaggacta taatacagct cagacaaagg gggataagca agccaacctg
     9001 ttgaggcaaa ctgccccacc accagtgaaa tattgcacaa catcgctccg acaaatgtta
     9061 accaaaaact aagtgaattt aaataaggat aagccacatc acgcgcacca atttgtaaag
     9121 gtactatcat attgagtagg gcaaaaacaa gcggcatagc aacaaagaaa atcataataa
     9181 cgccgtgtgc tgtaaaaatc tgatcgtaat gatcaggtgg caaaaatcca atggcattcc
     9241 ccgctgcaac tgcttgttga gagcgcatta agagtgcatc tgaaaaaccg cgtagcagca
     9301 ttacaaaggc taagattaaa tacataatac ctattttttt atgatcaact gaagtgaccc
     9361 attcgttcca gatataggac cattttttaa atatcgtcag taaggccaga accccgccta
     9421 atgctaatac cataaagatt ccagcgccca tagcaaaaaa gttatgaaaa ggaatggcgt
     9481 ccagggtcaa tttaccgaat aataaatttt gaagcataac tattccttat ggaatttaac
     9541 gatattttca cgtgggtgcg tccgacctat actttccata taaatcatta ttactttgtt
     9601 aaagaggtca ttttgcacgc tcgagaatag ttttggttta tcagcaatcg atgggtataa
     9661 caactgattg taagatgcat ctgtcaaccc attaggcgat ttttttactt ctgtaaacca
     9721 ttgttgtagt gttttaggtt caacaacatg gacttcaaaa tgcatgtccg aaaatccatc
     9781 cccattgtat tgcgtgttta aaccatcata aacgccaggt tcacttgcta aaagatgcag
     9841 acgggttcgc atcccagcca tggcataaat ctgactacct aactgcggaa taaaaaaggc
     9901 gctcatcggg acattatctg ttgttatcca atactctacc tgctgcccta atggaatttc
     9961 aagataattc actgttgcaa tgttttgttc tggatagata aatagccatt tccacggcag
    10021 agcaatcact tgaactaata agggtttttg agcctgacct gcaatgggtc gatagggatc
    10081 caattcatgc gtctttttcc atgtcacaat gcctaaaacc aatataatca cacaaggaat
    10141 accccaccat aatgcttcta aataaaaatt atggccccaa ttcggcttat aatcgcgggt
    10201 acggtgtgat acttgataat gataaacaaa ggtaatactc atgatgataa cgggtaacac
    10261 cacaatgagc attaaagcca aagtatcaaa aaacaactta cgctgctcga aggcaatcaa
    10321 acccttgggg ttaagcatcc cgataaaatg ctctttacaa ccacttaaag aaagcagtat
    10381 tagagaacct gagatgatga atacaacttt taagagccat cgaaaggcat ttatattaat
    10441 tgtagtagtt cgcgtccttg cgaccataaa gactcctggt taccctactt tcttcgttgc
    10501 caatataatg atacgattta aaataaaata caaagatttt ttaaattaaa catataatta
    10561 ctatggattg gtactatgac ctatataata tttgctcgct ggtttggttt ggtatcagtg
    10621 attctttctt taggtatttt atttaatcta gaggatgctc gtgatatggc taagcacatg
    10681 gtgaaatcag attctggtta catcatgggt ggagtacttc ccactgtctt cggcagttta
    10741 tctttaatgt tcgctaatag ttttgagtta ggatggacat taatcattac tttaattgga
    10801 gcaagtatgc ttttgattgg gatttttcga gtcatttttg ttaagcaatg gaaagcactt
    10861 ttatcacaat acatccaaga aatccctttt ttgtttagct tatttggctt gatgtttggt
    10921 gtaatcctac tctatgttgg ttttattgca aattcatttg cgttctagaa cgcatagggt
    10981 tgcgaagagg ccctaaagac tggttattgc tgagtaaggt tgttgcagta atcaaagtga
    11041 tcgctgccat aaaaattaga taataagccg gtgtgataag gcctttattt tctgtcagta
    11101 aagtaacaat caatggcgta ctgccaccaa ataaagcatt tcctaagtta taagaaaatg
    11161 cgatgccgct gtagcgaatg ttctcaggac aattttcaac aatagcggct agagtattgc
    11221 cttgttctaa tgagcttaga atagttgctg tacctaaaga taatagagcc aatattatcg
    11281 atttgaactg cagtaaataa aaacagggta aaaccaaaaa taaaatagct attgcggtga
    11341 ttatcagcat cttttttcgg gtaaaaaaat caccgcatag ccctgccaaa ggaacagtaa
    11401 acaacatgat aattagcata aagctctcaa tggttagcgc gttagccaat gaaaatccaa
    11461 tatagtgttc taaataattc ggtatatacg caaaaaacac ataataaaat gttgagccca
    11521 tgcaagtaag tcctaaaatt ttcagtaatt ggcggggtgc aaatttgata gctacaataa
    11581 aggggataga cacaaggcga tgtttttttt gcaacagtga ataaaccggt gtttcagata
    11641 atttaaaacg atagtaaatg attaatgagc ctggtaaccc tataaagaga aagggcaagc
    11701 gccatgccca attggtaatc gtctcttcgc taaaaaacaa atgtagaaac agtaatgtaa
    11761 ctgtagctaa taaaaaacct aaattcgcac ctagtgctgc aaaactcgtg gtaaaacccc
    11821 gtttgttaag tggcgctgat tcggcaaggt agattattat tccactgtat tccccaccaa
    11881 tagatacacc ttgtattaaa cgaagtaaga tgaatatgat tggggcagtt aatcctatct
    11941 cttcgtagga tggaataaca ccaagcaaaa aagttgagaa tgtgatgatt aagatagaaa
    12001 ttcttaatgt ttttgcacgg ccgcgagtat ctccaaaata accaaaaaaa ataccaccta
    12061 gtggtcgaca cagaaatccc gctgcaaaca cgataaaagc ctcaatggtt gcattgccag
    12121 ccgatgcctg cgggaaaaat ttagcaccga ttataggtgc catgaaaata aaaaggccaa
    12181 aatcaaacca ttctatggta ttgcccagac ttgaaatgag tatttctttt tttatcaatt
    12241 gataatcgct tcaggctatt attactaaat tcaatataca tgcatcttta aaaagataaa
    12301 tgctatttta ttcaaattat gggttaccta ttaaattttc attttagggc gcaccctgct
    12361 taacccccca tcgacagtca atacttgccc agtaatccaa ttgtttttag ggtctaaaag
    12421 aaaaataatg gtccgagcga tatcttctgg ggtaccaatt cggtctaagg cgtgcattgc
    12481 tttcgatgcc ttaaaagcca aagtatttga aataagctgc gcggtaagcg gggtatcagt
    12541 catccctggt gctaccacat taaagcgcaa atttaaatca gcataagttg atgctgcaga
    12601 ttgcgcaagc ccgataatcc ctgatttcgc ggctgcgatt gcttcatgat tggcaagccc
    12661 aacaagtgca gcagctgatg aaatcaatac tacagagccc ccacctttca tatgcattcc
    12721 agctgcacga atggttgcaa atgctgtagt taaggaagaa tcaataacag cttgatattg
    12781 ctcttttgca gtagtatgcg cgctttttaa taatagagag ccagcacaat taacaacgcc
    12841 atctattgga ccaaactgtt taaatacttc attgaccgct tcaaaatcag ttgcatccaa
    12901 tagtacatta ggttgaatct tgctgttatc tcgagcggta gtgaatacag tgtctccctg
    12961 agatattaaa gaattaacta ctgcctgccc tatagcactg ctcgctgcaa taactaaata
    13021 gttcgccata atctattttt gccttttgat gtgttattca accaaattaa ccaggttatc
    13081 atttaaatga taattacata aagactttaa aaaaaatggc tactgtaagg ttccatttaa
    13141 atgacaaaag agcgagattc aaacaccgat ttttgcagtc aatgtgggca tcaatcaatc
    13201 gttttttcaa ccccacccaa tgacacaagg gtgagaaaag tttgtaatta ctgcctaact
    13261 gttcattacc aaaacccaaa aattgctttt ggtacagtga caatgtttaa agataaattt
    13321 tattatgcaa ccgtgcgatt gaactgtagt tgggtttatg gatttatctt gctggttttt
    13381 cagaaaatgg taaatcttta gaggaaggtg cccagcgaga ggcttaagaa gaagcctttg
    13441 tcgaaataga tatttctcat cttgtaggaa cttactcttt gaaaggcatt aaccaaattc
    13501 atattatttt tgctgccact atgtcaaagc
//
LOCUS       DAXLDT010000081        13420 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXLDT010000081 DAXLDT010000000
VERSION     DAXLDT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13420
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.081"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..383)
                     /gene="icmD"
                     /locus_tag="ACF2SP_001897"
                     /gene_synonym="dotP"
     CDS             complement(<1..383)
                     /gene="icmD"
                     /locus_tag="ACF2SP_001897"
                     /gene_synonym="dotP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmD/DotP"
                     /protein_id="HFD2359679.1"
                     /translation="MNIKVIRKSGYKHWLVSAACLALLTLICQDAAAGVSLGKMASQI
                     TGSFTSLTKLITAGSYLAGLGFSIGAIMKFKQHKDNPTQIPIGTPIALIFIAAALLFL
                     PSILGVAGVTMFGGSGGKTAGPTGT"
     gene            complement(409..993)
                     /gene="icmC"
                     /locus_tag="ACF2SP_001898"
                     /gene_synonym="dotE"
     CDS             complement(409..993)
                     /gene="icmC"
                     /locus_tag="ACF2SP_001898"
                     /gene_synonym="dotE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmC/DotE"
                     /protein_id="HFD2359680.1"
                     /translation="MADPTCTGGTVACWIVSQANILANIANQLEPVQRLITGGAYLIG
                     CAFIFKAIYSLKVYGEARTMMSSNTSIKEPVMYLMVGALLIYFPSLVSSVLQTTFGYS
                     NPLAYSGGVSSGSDTISALFGSGSLVGRPLVMIIRVIGLIAFVRGWVLIARSASQGQP
                     PGGTGKGLIHVFGGILAINIVGTVDMINNTLYGT"
     gene            complement(1001..1810)
                     /gene="icmG"
                     /locus_tag="ACF2SP_001899"
     CDS             complement(1001..1810)
                     /gene="icmG"
                     /locus_tag="ACF2SP_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmG/DotF"
                     /protein_id="HFD2359681.1"
                     /translation="MMAEHDQNNDEYKFAELDSYDMDQAGESDLDSEASFQSGKEGLT
                     KKKDIKRNALIAIGAVVFIMVMYKIIGWMFFSDKSSQVTTKPAIPPVTQVATPQPVQT
                     IPTTTPIQQVQPTTIIEDDPDLKKKVSAIEMTQQSLRSEVNALGEQINAVNNNIKNLN
                     AQIVNLNQIIGNMSNQIARQSEVINVLMARTTPKKVVKVSRPTVQARIIYYIQAVIPG
                     RAWLIGSNGSTLTVREGSKIPGYGMVKLIDSLQGRILTSSGQVIKFSQEDS"
     gene            complement(1825..4971)
                     /gene="dotG"
                     /locus_tag="ACF2SP_001900"
     CDS             complement(1825..4971)
                     /gene="dotG"
                     /locus_tag="ACF2SP_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotG/IcmE"
                     /protein_id="HFD2359682.1"
                     /translation="MASKKENLKSLFSNTRTRVIIVFTAALLIIAVVIGFFKIRSATT
                     SSIAAAEVSTVPGGIQSIPGVLDPTAQYAKLQEEQNITQAQVAEKTGGSAIPTIIRTQ
                     ALGEGVGVIGSQSGVGFAALAQEELGGPQRSLWIQELQDGGCSKSVITKVMNQGAQLT
                     DLKAACSCVQLKDSGYGLQELEQVCECKELKAAGYNARQLKEAGYSAGRLRNCGFDAC
                     ELRNAGFTAQEMKDGGFSDGELKGAGFSDAEIAKASGLPDGITADDVRKAGCGAAALA
                     KLRQAGVSASAIRKISGCTAEQLKAAGYTAKELKDAGFSAADLKRAGFSAAELKDAGF
                     TARDLLNAGFTPADLAKAGFSNAQIKAAQAELPPGITPQDVKNAGCDVEALKKEREAG
                     VSAALIRQYAGCSAQALKAAGFTDADLASAGFTPAQISAATPLSDAEIKAAGCDPDKL
                     KKLFSAGVSAKRIKELNGCSAEALKAAGYDAQSLLAAGFTPQELLAAGFTPKQLEDAG
                     LNPASIIADGRVADCSVESLKKARAAGVSALTIKQTLGCSAAALKAAGYTAKELKDAG
                     FTAAELKAAGFSAKDLKDAGFTAKELRDAGFSAQELKDVGFSAKDLKDAGFSAAELKA
                     AGFTAAQLKAAGFSAKDLKDAGFSAAELKAAGFSAKELKDAGFSASDLKNAGFSAKEL
                     KDAGFSASDLKNAGFSASELKNAGYSADELKKAGYTSAELRNAGFSPQESAVAGLQGP
                     DLQQLDSSITGIPSIPGATPRPTTSDAASSAEQLQAILQKQNEQLAEQKYQQEIQQRT
                     SDMLTAATQLVQDWKKVETQVYTEGTEETKTSGGESSVPGAGTGAGANNQPVEQGASG
                     AQNQAIIKTGDIMFAVLDTAVNSDEPGPILATIVTGKLKGSKLIGSFNLPSNADKMVI
                     TFNTMSIPGAEKTISISAYAIDPNTARTALSSRTNHHYLMRYGSLFASSFLQGFGNAF
                     QSANTTITIGGTGGGNNITVANGVGRSTLENAVIGLATVGKAWSQQAQQLFNTPTTVE
                     VYSGTGLGILFTQDVTTI"
     gene            complement(4976..6058)
                     /gene="icmK"
                     /locus_tag="ACF2SP_001901"
                     /gene_synonym="dotH"
     CDS             complement(4976..6058)
                     /gene="icmK"
                     /locus_tag="ACF2SP_001901"
                     /gene_synonym="dotH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmK/DotH"
                     /protein_id="HFD2359683.1"
                     /translation="MKKYDQLCKYCLVIGMTFSMSCSTYAADQSDDAQQALQQLRMLQ
                     QKLSQNPSPDAQSGAGDGGDNAASDSTQQPNQSGQANAPAANQTANAGGDGQIISQDD
                     AEVIDKKAFKDMTRNLYPLNPEQVVKLKQIYETSEYAKASTAGTPPKPTATSQFVNLS
                     PGSTPPVIRLSQGFVSSLVFLDSTGAPWPIAAYDLGDPSSFNIQWDKTSNTLMIQATK
                     LYNYGNLAVRLRGLNTPVMLTLIPGQKAVDYRVDLRVQGYGPNAKSMPTEEGIPPSAN
                     DLLLHVLEGVPPPGSRRLVVSGGDARAWLSNEKMYVRTNLTILSPGWLASMTSADGTH
                     AYEMQKSPVLLVSWHGKVMQLKVEGL"
     gene            complement(6061..6699)
                     /locus_tag="ACF2SP_001902"
     CDS             complement(6061..6699)
                     /locus_tag="ACF2SP_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system apparatus protein
                     IcmL/DotI"
                     /protein_id="HFD2359684.1"
                     /translation="MAEDALTVVALRNKFYKDSQRKVILALLIAIVVNVVLASLVVYM
                     ITHPPAPKYFATSINGRITPLFPLDEPNQSDSAVLQWANQAAIAAFTYNFVNYRDELQ
                     ASSGFFTAEGWDQFLGALEQSNNLDAVKAKKLVVSAVATRAPIILQKGVLNGRYSWRV
                     QMPILVTYQSASEFTQQNNVVTMLITRVSTLNSPRGIGISQFVVGPASGGVS"
     gene            complement(6715..6999)
                     /gene="icmM"
                     /locus_tag="ACF2SP_001903"
     CDS             complement(6715..6999)
                     /gene="icmM"
                     /locus_tag="ACF2SP_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmM/DotJ"
                     /protein_id="HFD2359685.1"
                     /translation="MSRETWALIKRNKNFNVHIYRRGLTLLILSLGLSCGLSGLIYYV
                     HMHEPERDFYATSGITPPIQLKPMPTPNMSSEALLPPDPPDENLQRIIPQ"
     gene            complement(7011..7580)
                     /gene="icmN"
                     /locus_tag="ACF2SP_001904"
     CDS             complement(7011..7580)
                     /gene="icmN"
                     /locus_tag="ACF2SP_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmN/DotK"
                     /protein_id="HFD2359686.1"
                     /translation="MRSLRTNYIYVLFKTTGLLFLLLLSACNRSGYIPENEVPKLPCR
                     VDGACDATIIKMMTDLNKKGIKVASVGQNYLISIPASALFADQSPRLNWASYSLLNEV
                     AAFLKQFRKIAITVTSYSSKYVSVKRERALTLARSRVVSEYLWSQGVDSRIIFTQGLG
                     SDKPITSYTLGGDRSPNARVEITFRRAVA"
     gene            complement(7984..10335)
                     /gene="icmO"
                     /locus_tag="ACF2SP_001905"
                     /gene_synonym="dotL"
     CDS             complement(7984..10335)
                     /gene="icmO"
                     /locus_tag="ACF2SP_001905"
                     /gene_synonym="dotL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmO/DotL"
                     /protein_id="HFD2359687.1"
                     /translation="MMRGIDSRHELDPTLLLRDTRTFTQRLADFFADPTNISIVLISL
                     AAVSYYFSEAATFLLIMGGIFFLYSYTRKQKLPFRLPQISRAKDYNDLKPGINKPNIA
                     RGITFFGNDRKTGEELWFANDDMRTHALIFGSTGSGKTETLVSLSYNALVQGSGFIYV
                     DGKGDNSLYAKVFSMVRSMGREDDLLLINFMTGARDIVGPQEKRLSNTLNPFCQGSSS
                     MLTQLVVSLMGSSGQSSDGDMWKGRAIAFVEALMRLLVYMRDEGAILLDANTIRNYFD
                     LQRLESIVIDKLFPRDDQESVNIETIPKLVTDPLRNYLNTLPGYNKEKKGKQVSQVLE
                     QHGFITMQLVRSFSSLADTYGHIIRTNLAEVDFKDVVLNRRILVVLLPALEKSPDELS
                     NLGKIIVSSLKAMMAAGLGEEVEGDYRDVILRKPTNAPTPYMCILDEYGYYAVQGFAV
                     VPAQARSLGFSAIFAGQDLPAFQKASKEEAASIGANTNIKICMKLEDPTETWDFFTKT
                     AGEAYVTKVDSFQTKETSIANSYMDTKSSSFEKRARVDLLDLKEQTEGEAHIFFKSKI
                     VRARMFYANPKPVKQLKINQFLKVEPPPDDYLMKLQKQLASFQSILESGDLSINKAVE
                     NEEITLISKALKESTIVEPIERGVAALIAFHGQNEPEPVEDIVEEEVEGALTIFSKLR
                     IDPNAPPILVADKEVFSEPLLPINETRNQMITIERLAGAKDKYAGTVANELIKDFQIA
                     TSYPPEERDVIDVQELTGIIRDLSAKISAEREKANKKAAEELT"
     gene            complement(10332..11462)
                     /gene="icmP"
                     /locus_tag="ACF2SP_001906"
     CDS             complement(10332..11462)
                     /gene="icmP"
                     /locus_tag="ACF2SP_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system coupling complex
                     protein DotM/IcmP"
                     /protein_id="HFD2359688.1"
                     /translation="MAQQQQQSGSDNSMAPVWIIILLFITAYFVWALAHQYIVSFVFT
                     INIWQARLVNLFLNNQLLANQIYLMQTLDPNTVNWDQMVTVMRAVGDYMRYPVICILV
                     VLAFVLYNSNVTLKYRKTYDMKSLRAQEQFNWPAIMPIVKEDLVSQDVNKGPWAMALT
                     PMEFARKYNLLRKDDALLDNPVPGEEMTAGIRRGDAKRVFTMQLGPYWDGFERCSPQA
                     YALSAVFIARMNRDRDAANNILKVLDKTFVDGKPDFSVARPVMKKYQNSELVQEVVAK
                     HAYVLTVIASLLEAAREDGVVPSSEFLWLKPVDRRLWYMLNCVGRQTPYSEVAGPFAH
                     WKAEKEMGRRSLVPMIDEAIRALEIAVKEVRLTPRQMEELEP"
     gene            complement(11649..12224)
                     /gene="icmQ"
                     /locus_tag="ACF2SP_001907"
     CDS             complement(11649..12224)
                     /gene="icmQ"
                     /locus_tag="ACF2SP_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm secretion system protein IcmQ"
                     /protein_id="HFD2359689.1"
                     /translation="MKDQLSDEQKETILKALNDAIEKGPWDKSNFLRVIGKKLIAIRD
                     RFLKRIGAASQARLKAESHLANRIALRSGQQEIYVSLYSSDGSNLQSWEKIVGSLPRQ
                     MISRPIYADEEDIKAILKTKENKQNEAYVAIYISQSDILHLSADKAPVDKLGKPLLTL
                     KDKSISLENISRFVHVSGVYRYSNGRLIKNA"
     gene            complement(12315..12677)
                     /gene="icmR"
                     /locus_tag="ACF2SP_001908"
     CDS             complement(12315..12677)
                     /gene="icmR"
                     /locus_tag="ACF2SP_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmR"
                     /protein_id="HFD2359690.1"
                     /translation="MGNNTDDSARNPFGFYTPPRVKEIGESDVTDATLGSVYSEIISP
                     VKDCILTVAKAVSFNPGGKDNTDAVEVLTELNTKVERAALNQPILTAKTERMFGAAES
                     EKSSEQPSHEERGFKLSS"
     gene            complement(12777..13121)
                     /gene="icmS"
                     /locus_tag="ACF2SP_001909"
     CDS             complement(12777..13121)
                     /gene="icmS"
                     /locus_tag="ACF2SP_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmS"
                     /protein_id="HFD2359691.1"
                     /translation="MERDISKCMAKIAASMNAKFYLNDRFVSFDEVFSETGLLPAIAK
                     RADQLCSLCLGYGLGATYDESEGALLGIRVVFDEVTPNVLRLLCMTDVMNELIQGGPS
                     RDYTPLDELMYD"
     gene            complement(13122..13382)
                     /gene="icmT"
                     /locus_tag="ACF2SP_001910"
     CDS             complement(13122..13382)
                     /gene="icmT"
                     /locus_tag="ACF2SP_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmT"
                     /protein_id="HFD2359692.1"
                     /translation="MAGGFSATSHWRDSARSARFFVVDARAAFPIFLFLMHIRVWTGV
                     LVLVSAIFFGIIEHYGFTVPVFLRWLRSFLAGSVRSARPWWR"
BASE COUNT         3926 a         3078 c         2333 g         4083 t
ORIGIN      
        1 attgttccag taggtccagc agttttacca ccactaccac cgaacatagt tactccggcc
       61 actcctaaaa tagaaggaag gaataataat gctgcagcga taaatatcaa agctattggt
      121 gttccaattg gaatttgagt aggattatcc ttgtgctgtt taaatttcat aatagcacca
      181 atagaaaaac ccaatccagc caagtaagag cccgctgtaa tcaatttagt aagacttgta
      241 aatgaacccg taatttgtga tgccatttta cctaacgata cacctgctgc agcatcctga
      301 caaattagag tcaataaagc caaacaagct gcgcttacta gccagtgttt ataacctgat
      361 ttacggataa ccttaatgtt cacaattctc tcctgttttt aaattagctt aagtaccata
      421 taaagtattg ttaatcatat caactgtacc aacaatattg attgccaata ttcccccaaa
      481 tacatgaatc aaacctttac ctgtacctcc tggaggttgc ccctgcgacg cggaacgcgc
      541 aattaatacc caccctctta caaatgcaat tagaccaata acccggataa tcatcaccaa
      601 aggtctgcct actagactgc cactaccaaa taaggctgaa atagtatcgc ttccactact
      661 cacacctcct gagtaggcta atggattgct ataaccaaaa gtcgtttgca gtacggagga
      721 aaccaatgac gggaaataga taagtaatgc acctaccatc aaatacatca ctggctcttt
      781 gatactcgta ttactcgaca tcattgtcct ggcttctcca taaaccttta agctataaat
      841 tgccttaaat ataaaagcac acccaatcaa ataagcgcct ccagtaatca gcctttgaac
      901 aggctccaac tgattagcaa tattagctaa tatgttagct tgactcacta tccagcatgc
      961 tactgtgccg ccagtacaag tcggatcagc catatacgcc tcaactatct tcttgactaa
     1021 acttaatcac ttgtccagaa cttgttaaaa tacgaccttg caaagaatca attaatttaa
     1081 ccattccata tcctggaatt tttgatccct ctctaactgt aagtgtagaa ccatttgagc
     1141 caattagcca agcccgacct ggaattactg cttgtatgta ataaataatt cttgcttgaa
     1201 ctgttggccg agacactttt acaactttct taggtgttgt acgcgccatt aagacattaa
     1261 tcacttctga ttgtctggct atttgatttg acatattacc aataatttga ttcagattta
     1321 caatttgagc atttaaattt ttgatattat tatttactgc attgatttgc tcaccaaggg
     1381 cattgacttc cgaacgaaga ctctgctgtg tcatttctat tgctgaaact ttcttcttta
     1441 gatcggggtc atcttcaata atagtcgtag gctgaacttg ttgtatggga gtggtggttg
     1501 gtattgtctg tacaggttgt ggtgttgcta cctgtgtcac tggaggaata gcgggctttg
     1561 tggttacttg gctggattta tctgagaaaa acatccaacc aattatttta tacatcacca
     1621 ttataaatac aactgcccca atagcaataa gagcgtttcg cttaatatct tttttctttg
     1681 ttaatccctc tttcccagat tgaaacgaag cttctgaatc gagatccgat tcaccagcct
     1741 gatccatatc gtatgagtca agttctgcaa atttatattc atcattattt tgatcgtgct
     1801 ctgccatcat tatccgccat caaattaaat tgttgtaaca tcctgggtaa ataaaatacc
     1861 caaacctgta ccagaataaa cttccacagt tgttggtgta ttaaacaatt gttgcgcctg
     1921 ttgactccat gcctttccaa cggtagccaa tcctataacc gcattttcca aagttgagcg
     1981 accaacacca ttggctacag taatattatt accaccacca gtacccccaa tggtgattgt
     2041 tgtatttgcc gactggaatg catttccaaa tccttgtaag aaagaagaag caaacaggga
     2101 accatatcgc attaaatagt gatgattagt tctgctggat agggctgttc ttgccgtgtt
     2161 tggatcaatt gcataagccg atatggatat agttttttct gctcctggaa ttgacattgt
     2221 attaaaggta atcaccatct tgtccgcatt ggacggtaag ttgaagcttc ctattaattt
     2281 tgagcctttt agcttaccag taacaatagt tgccaatatt ggacctggct catcactatt
     2341 cacagcggta tccagaaccg caaacataat atcgcccgtt ttgataatag cctgattctg
     2401 agcaccactt gcaccttgct ctactggttg attattagca cctgcaccag tgcctgctcc
     2461 tggaactgag ctttcaccac cagatgtttt tgtttcttct gtaccttctg tatacacctg
     2521 ggtttcaacc ttcttccagt cttgaactag ctgagtcgcc gctgtgagca tgtcagaagt
     2581 tctttgttga atttcttgct gatatttttg ctcagccaat tgttcatttt gcttttgcaa
     2641 aatggcctga agctgctcag cactagaggc agcatcactg gttgttggcc ttggagtagc
     2701 gccaggaatt gagggaattc ctgtaatgct tgagtcgagt tgttgcaaat caggcccttg
     2761 taaccctgca acggctgatt cttgtggaga aaatccagca tttctcagct cagctgaggt
     2821 atatccagct ttttttagct catcagctga atatccagca ttctttaatt ctgaggcact
     2881 aaatcctgcg tttttcaaat ctgaggcgct aaatcctgca tctttcaatt cttttgcgct
     2941 aaacccggca tttttcaaat ccgaggcgct aaacccggca tccttcaact cctttgcact
     3001 aaatccggct gcttttaatt ccgctgcaga gaaaccagca tcttttagat cttttgcaga
     3061 aaacccagca gcctttagct gggctgcagt aaatccagcc gcttttaatt cagcagcaga
     3121 aaacccagcg tcttttaagt cttttgcact aaaacccaca tcttttaatt cctgggcgga
     3181 gaaacccgca tcacgcaatt cctttgctgt aaaaccggca tcttttagat ctttcgcact
     3241 aaaaccggct gcttttaatt cagcagcagt aaacccggcg tctttcagct cttttgcagt
     3301 ataaccagct gctttcaatg ctgcagcgga acatcctaat gtttgcttta ttgtcaaggc
     3361 actaacacca gctgccctcg ctttttttaa tgactctacg ctgcagtctg cgacacggcc
     3421 gtctgcaata atcgatgcgg gatttaatcc agcatcttcc aactgctttg gtgtaaatcc
     3481 tgccgcaagc aattcttgag gtgtaaatcc tgccgcaagc agggattgcg catcataccc
     3541 cgcagctttt aaagcttccg cactgcaacc attgagttct ttgatgcgtt ttgcagatac
     3601 accagcagaa aataattttt tcagtttatc aggatcacaa ccagcagcct ttatttccgc
     3661 atcactcaaa ggagttgctg cactgatttg agctggtgta aatcctgcgc tcgccaaatc
     3721 cgcatcagtg aatcctgcag ctttgagggc ctgtgcgcta catcccgcgt attgtctgat
     3781 taaagcagca ctaacacctg cttccctttc ctttttcaaa gcctccacat cacaaccagc
     3841 attcttcaca tcttgcggag taattccagg cggaagctca gcctgagcag ctttgatttg
     3901 agcattagaa aatcctgcct ttgccagatc agctggtgta aatcctgcgt ttaacaaatc
     3961 tcttgcagta aatccggcat cttttaattc tgcagcagaa aaacctgctc gttttaagtc
     4021 cgcagcacta aaacctgcat cttttaactc tttagcggta taacctgccg ctttcaactg
     4081 ctcagcggtg caaccactta tttttctaat cgcggacgca ctaacccctg cctgacgtaa
     4141 tttggccaat gcagcagctc cacatcctgc tttgcgcaca tcatctgcag ttataccatc
     4201 tggtaagcca cttgcctttg caatttcagc atcagagaac ccagctcctt ttaattcccc
     4261 gtctgaaaaa cctccatctt tcatttcctg agctgtaaaa cccgcattac gtaattcaca
     4321 cgcatcaaac ccacaatttc gcaatcgacc tgcactataa cctgcttctt tcaactgcct
     4381 tgcattataa ccagcggctt ttagctcttt acactcacag acctgctcta attcttgcaa
     4441 accgtatcca ctatctttta actgaacgca gctacaagcc gctttcaaat cagttaattg
     4501 agctccctga ttcataactt tcgtaatcac agatttgcta caaccaccat cttgtaactc
     4561 ttgtatccat aaacttcgtt gaggaccacc taactcttct tgagccaaag cagcaaaacc
     4621 cactccactt tgggaaccga taacaccaac tccttctcct agtgcttggg tgcggataat
     4681 tgttggaata gcactcccac ctgttttttc agcgacttgt gcctgggtta tattttgttc
     4741 ttcttgcaat ttggcatatt gagcggttgg atctaaaact cctggaatag attgaattcc
     4801 tcccggcact gtagacactt cagctgcagc aatcgaactg gtagtagcgc ttcttatttt
     4861 aaaaaaacct attactaccg cgatgattaa taaagctgct gtaaaaacga taatcactcg
     4921 agtccgagta ttggaaaaca gtgactttag attttctttt ttgctcgcca tttgcttata
     4981 acccttctac cttgagttgc atgacttttc catgccaaga cactaataaa accggagatt
     5041 tttgcatttc ataagcatgc gttccatctg cgctagtcat actggctaac cagcccggcg
     5101 acaatatagt gagattcgtt ctaacataca ttttttcatt agataaccaa gctcgagcat
     5161 ccccaccact aaccactaga cgcctgcttc ctggcggagg aacaccttct aacacatgaa
     5221 gaagtaaatc attcgctgat gggggaatgc cttcttcagt tggcatactt ttagcgttgg
     5281 ggccatagcc ttgaactctc aagtctactc gataatccac ggctttctgc ccgggtataa
     5341 gagtaagcat gacaggggta tttaatcctc ttaaccttac tgccaaatta ccatagttat
     5401 atagcttcgt agcctgaatc attaaagtat tactggtttt atcccattga atattaaacg
     5461 aagagggatc acccaaatca tatgcagcaa taggccaagg ggctcctgtt gaatccagga
     5521 acactaagga agacacaaaa ccttgtgata atcttattac cggcggtgtt gagcctggag
     5581 acagattaac aaattgtgac gtcgctgtag gtttaggtgg agtgcctgcc gttgaagctt
     5641 tggcatactc ggaagtttca taaatttgct ttaatttgac tacttgctca ggatttaagg
     5701 gatataagtt gcgtgtcata tccttaaatg ccttcttatc aataacttcg gcatcatctt
     5761 gactaattat ctgtccatca ccgcccgcat ttgctgtttg attcgcagcc ggagcattag
     5821 cctgaccgga ttgattaggc tgttgcgttg aatcagaagc tgcattatca ccaccatcac
     5881 cggctcctga ctgtgcatca ggggaagggt tttgtgataa tttctgttgt agcattcgca
     5941 gttgctgcaa cgcttgctga gcatcatctg attgatcagc cgcataggta ctgcatgaca
     6001 tactgaatgt catgcctatc accaaacaat atttacataa ttgatcatat ttcttcatca
     6061 ctaggatacc ccaccgctgg caggtccaac aacaaattgt gaaataccta tccctctagg
     6121 cgaattcaac gttgaaactc tagttattaa cattgttact acgttatttt gttgtgtaaa
     6181 ttcactggca ctttgatagg tgactaatat tggcatttgt accctccagg aatacctacc
     6241 attgagcact cctttttgca aaatgattgg ggctcgggtt gctactgctg acacaactaa
     6301 ttttttcgct ttaaccgcat caaggttatt ggattgctct aatgcaccta aaaattggtc
     6361 ccagccttcc gcagtaaaaa agccggaaga agcctgtagc tcatcgcgat aatttacgaa
     6421 attataggta aatgcggcaa ttgctgcctg attagcccat tgtagtacag cggagtccga
     6481 ttggtttggt tcatccaaag gaaataaagg ggtaatacga ccgttaatac ttgttgcaaa
     6541 gtactttgga gcaggtggat gagtaatcat atagacaaca agcgatgcca agaccacatt
     6601 taccacaata gcaatcaata acgccaaaat gactttgcgt tgactgtctt tataaaactt
     6661 atttcttaat gcaactacag ttaaggcatc ttcagccata atatcctcta aaaattattg
     6721 cggtataatc ctttgtaagt tttcatctgg tggatccggc ggaagcaatg cttcagatga
     6781 catgttagga gtcggcattg gcttaagctg gataggagga gttatgccgc tggtcgcata
     6841 aaaatctcgc tcaggttcat gcatatgaac ataataaatc aacccagaca aaccacaact
     6901 caatccaaga gaaagaatta gcagtgttaa gccacgtctg taaatatgga cattaaaatt
     6961 cttatttcgt tttattaaag cccatgtttc tcgactcatt tttacctcaa ttatgccaca
     7021 gcccgcctaa atgtgatctc caccctggca ttaggtgatc tatcaccacc aagagtataa
     7081 ctagttatag gtttatcact acctaaccct tgtgtaaaga tgattctgct atcaaccccc
     7141 tgagaccata ggtattcact tactactctt gatctcgcca atgtcaatgc ccgttctctt
     7201 tttaccgaca catatttact actgtaactg gttaccgtta tagcaatttt acgaaattgt
     7261 ttaagaaacg ccgcgacctc atttaaaagg ctataggaag cccaatttaa ccgaggagat
     7321 tgatcagcaa acagagcgct agcaggaata ctaattaaat aattttggcc tactgatgca
     7381 actttaatcc cttttttatt taaatcagtc atcattttta ttattgttgc atcgcacgca
     7441 ccatcaacac ggcatggtaa tttgggcact tcattctcag ggatataacc agaacgatta
     7501 caagcactca gcaacaataa gaataataat ccagttgttt taaacaatac atagatgtag
     7561 ttagtacgaa gtgatctcac ctgttatccc catctttgaa aatagaccaa ttgtgttatc
     7621 tatgtctaac tatctcaaac attccatttg ataataaaaa catacaaatc attacgttac
     7681 aatgaattta ataaaattgc cattttttta aataatttat ttatttaaga tggggttgac
     7741 taattaaggc aaatcaacat atgccaaatt aatctatgag taaactgata attaccctgt
     7801 taattcagaa tacaaaaaat tctcccaaag aggaaccaca cagaatatca gcctgataga
     7861 tcaaagttta aagaaacaag gtcactgccc ggattataaa aaactcagag taacttacat
     7921 atggagctct cttcatgccc aatagcacag gaacacctct acctccaatt gccgactgag
     7981 tctttatgtt aattcctctg cagccttttt attggctttc tcacgttctg cggaaatttt
     8041 agctgataaa tctcttataa taccagtaag ttcttggaca tcaatcacat ccctttcttc
     8101 aggaggatag ctagtcgcta tttgaaaatc tttgattaat tcattagcta ctgtaccagc
     8161 gtatttgtct ttagcccctg ccaaacgctc aatcgttatc atttgatttc gtgtttcatt
     8221 aataggtagt aatggctcag agaaaacttc tttgtcagcg actaatattg gaggagcatt
     8281 agggtcaatt cgtagtttgc tgaaaatagt taatgccccc tcaacttctt cctctacaat
     8341 atcttccaca ggctcgggtt cattttgacc atgaaatgca attaaagcag caacgcctcg
     8401 ttcaattggc tctactatag tagattcttt taatgcctta ctaatgagag tgatttcttc
     8461 attctcgact gccttattga tactcaagtc accactttca agaatgctct ggaaagaagc
     8521 caattgtttc tgtagcttca ttaaataatc atctggcggc ggctccactt ttaaaaattg
     8581 gttaattttc aattgcttaa ccggtttggg attggcataa aacatacgag cacgaacaat
     8641 tttggatttg aaaaatatat gcgcctcacc ttcggtttgc tcttttaaat ccaggaggtc
     8701 tacccgagct ctcttttcaa aagaagagct tttagtatcc atatagctgt tggctatgct
     8761 ggtttctttg gtttgaaacg aatccacttt tgtaacataa gcttcgccag ctgttttagt
     8821 gaaaaaatcc caggtttcgg taggatcttc cagtttcata catattttaa tgtttgtgtt
     8881 tgcaccaatg gacgcagcct cttctttgga tgctttttgg aatgcaggta agtcttgtcc
     8941 agcaaaaata gcagaaaatc ctagagaacg agcttgcgca ggcactaccg caaatccttg
     9001 tactgcgtaa taaccatact catcaagaat gcacatataa ggagttgggg cattcgtggg
     9061 ttttcttaga ataacatcac gataatcacc ttctacttct tcacctaaac ctgccgccat
     9121 catagctttt aatgatgaaa caataatctt gccgaggtta gataactcat ccggtgattt
     9181 ttccagagca ggtaataaaa ccactaatat acgtctgttg agcaccacat ctttaaaatc
     9241 gacttctgct aaattggtac gaataatatg accataagta tctgccagag atgagaaact
     9301 tcttaccagc tgcatggtaa taaaaccatg ttgttccaaa acttgggata cctgtttccc
     9361 ctttttctcc ttattatacc caggcaaggt atttaaatag tttcttaaag gatccgttac
     9421 cagttttgga atggtttcaa tattgacgct ttcttgatca tctctgggaa aaagtttatc
     9481 aattacaata gattctagtc tttgcaaatc aaaataattt cgaatagtat tcgcatcaag
     9541 aagaattgcc ccttcatcac gcatatacac caataaacgc attaatgctt ctacgaaagc
     9601 tatggctcgc cccttccaca tatcaccatc agatgattgg cctgaagatc ccatcaaact
     9661 gactaccaac tgagttagca tactggaaga accttggcag aatgggttta gtgtattgga
     9721 aagtcttttt tcctgcggac cgacaatatc gcgcgctcca gtcatgaagt tgattaagag
     9781 caaatcatct tcacgcccca tgctccttac catagaaaag actttagcat acagagagtt
     9841 atctccttta ccatcaacgt agataaaccc gctcccctgt accagggcat tatacgacag
     9901 agaaaccaaa gtttctgttt taccactacc cgttgaaccg aaaattaacg catgggtacg
     9961 catatcatcg ttagcaaacc aaagttcctc gccggtcttt ctgtcattac caaaaaaggt
    10021 aatacctcga gctatattcg gtttattaat acccggtttc aaatcgttgt aatccttcgc
    10081 acgcgaaatt tgaggcaatc gaaatggcaa cttttgcttt cgggtatagc tataaagaaa
    10141 aaatatgccc cccataatca gcaaaaatgt tgctgcttca gagaaataat atgaaacagc
    10201 tgctaatgaa ataagaacaa tagaaatgtt ggtcggatct gcaaaaaaat ctgccaaccg
    10261 ctgcgtaaaa gtacgtgtat ccctaagtaa aagggttgga tctaattcat gacgagaatc
    10321 aataccccgc atcatggctc taattcctcc atttgacgtg gtgttaatct tacctctttt
    10381 actgcaattt ctagagctct aatcgcttca tctatcattg gcaccaaaga gcgtcttccc
    10441 atttcctttt cagctttcca atgtgcgaaa ggacctgcaa cttcagagta tggagtttgc
    10501 ctgcctacac aattcaacat ataccataaa cgtctgtcga ctggtttgag ccataaaaac
    10561 tctgagctgg gaaccacgcc atcttctcgt gctgcttcaa gcaaagaagc aatcactgtg
    10621 agcacataag catgcttggc tactacttcc tgaaccaact ctgagttttg gtattttttc
    10681 attactggcc ttgcaacaga aaaatccggt ttcccatcca caaatgtttt atctaaaact
    10741 ttaaggatat tatttgctgc gtctctatct ctattcattc ttgctataaa tacggcagac
    10801 aatgcgtaag cctgaggaga gcaacgttca aaaccatccc aataaggacc caattgcatg
    10861 gtaaatactc gtttggcatc gccacgccga atccccgctg tcatctcttc gccaggcaca
    10921 ggattatcca ataatgcgtc gtcctttctc aataaattat attttcgcgc aaactccatg
    10981 ggtgttaaag ccattgccca aggcccttta ttgacgtctt gactaaccaa atcctcctta
    11041 actataggca tgatggcagg ccaattaaat tgttcctgag cccgcaaact tttcatatca
    11101 taggttttgc gatattttaa agtcacattt gagttataga gaacaaaggc taacactacc
    11161 aggatacaaa taaccgggta acgcatataa tcaccgaccg cacgcatcac agtcaccatt
    11221 tgatcccaat tgacagtgtt aggatcaagt gtctgcatca gataaatttg attggccaag
    11281 agttgattat ttaaaaacaa attaactaat cttgcttgcc agatattgat agtaaataca
    11341 aaactaacaa tgtactgatg agccagcgcc caaacaaaat aagcagtaat aaataacagt
    11401 atgatgatcc atactggcgc catggaatta tctgaacctg attgctgctg ttgttgtgcc
    11461 atctctatat acaattagtt aatagattct atatatcaag tatatactgc tcagagctat
    11521 ttttgcgaga ttttcgtgta ctatatcaaa atatgaatac aaggatgatt aaataaagcg
    11581 taaagacagt tagagtagaa ataaattttt aacacgctag aaatcattat aaagaaaacc
    11641 gaaacggcct atgcattttt aattagacgg ccgttggagt atcggtatac gccactcaca
    11701 tgaacaaagc gactgatgtt ttccagactg atggatttat ctttaagcgt taataacggt
    11761 ttacctagtt tatctaccgg agccttatca gcggataaat gcaagatatc tgattgactg
    11821 atgtaaattg ccacatacgc ttcattttgt ttattttctt ttgtttttaa tatcgctttt
    11881 atgtcttctt catctgcgta aataggtcgt gaaatcattt gccggggcaa actacccact
    11941 attttttccc aagattgaag attgcttcca tctgaggaat ataatgaaac atatatttcc
    12001 tgctgaccgc tccttaaggc aattcgattt gcaagatgag actctgcttt taacctagcc
    12061 tgactagcgg cacctatccg ttttaaaaaa cgatctcgaa ttgcaattaa ttttttacca
    12121 attaccctta agaaattgga tttatcccat ggaccttttt caatagcatc gtttaaggcc
    12181 tttagaatgg tttctttttg ttcatccgag agttgatctt tcataacttc aatacttatg
    12241 ggaaccaaga attaggggaa atataaagaa agtataacag catttgtaaa aatcactgaa
    12301 atgaaattaa tctttcaaga tgataatttg aaaccacgtt cctcatgact tggttgctca
    12361 gacgattttt cagattcagc agcaccaaac attctttctg tctttgctgt taaaatcggt
    12421 tgatttaagg cggctctctc tacttttgta ttaagctctg ttaacacctc aactgcatcc
    12481 gtgttatcct tccctccggg attaaagcta actgcctttg caacagtcaa aatacaatct
    12541 tttaccggtg aaataatttc actatagaca ctgccaagag tagcatcagt tacatcagat
    12601 tcacctatct ctttaacacg aggtggcgta taaaaaccaa atggatttcg tgcactgtca
    12661 tcagtattat tacccattat taccactcct gagctcaatc tcttacttta catatatcaa
    12721 aatatatctt tcaatatatc ctgatccgat aaaactttct aattttttac aaacaactaa
    12781 tcatacatta actcatccag gggagtataa tcacggcttg ggcccccttg aataagctca
    12841 ttcataacat ccgtcataca taacaagcgt aatacattcg gcgtcacttc atcaaaaacc
    12901 actctaatac ctagtaacgc tccctcagac tcgtcataag ttgctcccaa accataacct
    12961 aaacacaagg aacacaattg atccgctctc ttggcaatgg ccggtagcaa acccgtttct
    13021 gagaaaactt catcaaaact tacaaatcta tcgttcaggt aaaatttcgc attcatgctt
    13081 gctgcaatct ttgccataca cttgctaata tctcgctcca tttatctcca ccaaggtcta
    13141 gcagatctaa ctgaaccagc taaaaaactt ctgagccagc gtaaaaaaac cgggacagtg
    13201 aaaccataat gttcaattat cccaaaaaaa attgcagaaa ctaagaccaa aacacctgtc
    13261 catactctta tatgcattag aaataaaaaa attgggaatg cggccctcgc atcaactaca
    13321 aaaaaccgcg cgcttctcgc tgagtcacgc cagtggctcg ttgctgaaaa cccacctgcc
    13381 atttttaaac ctcaaaaaat catcctttaa aaaaagtata
//
LOCUS       DAXLDT010000082        13365 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXLDT010000082 DAXLDT010000000
VERSION     DAXLDT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.082"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            1..1095
                     /locus_tag="ACF2SP_001911"
     CDS             1..1095
                     /locus_tag="ACF2SP_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFD2359693.1"
                     /translation="MATVACATQVSLRDKIGQMLILGFQGKVVDEKSPIAQAIEKNNI
                     GGVILFDFNQQSQTFDKNIESPEQVNQLNNQLQTLTQRANRKYHRDNLPLLISVDYEG
                     GNVNRLHPRYGFPEMPSAKDIGSMSLERADASAELMANTLKSTGFNLNFFPELDVNIN
                     PDNPIIGKKDRSFSSIPEIVTQYAQLYTEQFIKHQIHCSYKHFPGHGSSLSDSHLGFV
                     DISDTWSEQELIPFLQLLSQPNHCDMVMIAHIVNRKLDMTGLPASLSYQIINGLLRHD
                     LKFNGVVITDDMQMKAITNYYGLETAVTLSINAGADMLIFGNQLVEKFQDSTEIIDMI
                     EQKVRSGEISEQRINEAYQRIVKMKRSFNY"
     gene            1235..1960
                     /locus_tag="ACF2SP_001912"
     CDS             1235..1960
                     /locus_tag="ACF2SP_001912"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFD2359694.1"
                     /translation="MKNALKREDAANKKLGVLIYENVQPMDVIGPWEVFATWKTILDA
                     PIDMFLVAESSNEVCCANQISLKPHCEFNNSPQFDYLLVPGGAGRREQVNNPRLISFL
                     KKQAQNTDYLLSVCTGAFLLHAAGLLIHHEATTYWRAMPELKSFEDVQLVEKRIVKSG
                     KVWTTGGISSGIDLAFEFISTIAGNDVAGKVQLLFEYFPECKLYSQPAMVSELPSYYA
                     TKKDEAVLEMNLPDYIKEYFKKS"
     gene            complement(2049..2672)
                     /gene="hisIE"
                     /locus_tag="ACF2SP_001913"
     CDS             complement(2049..2672)
                     /gene="hisIE"
                     /locus_tag="ACF2SP_001913"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFD2359695.1"
                     /translation="MSDLDINSLDWQKMNGLLPAIIQNAENGKVLMLGYMNQEALIAT
                     LTTGQLTLYSRSRKRLWRKGESSGNSMALQHISADCDNDSLLIQVIPKGPACHLGYST
                     CYQPHNNYTLGFLDGLIELINERAEEKNPNSYTAQLLDSGVNRCAQKLGEESVEAVIA
                     AVSNNKEELINEASDLIYHLLVLLKACELSFYDVLLCLQARDRSVHT"
     gene            complement(2669..3436)
                     /gene="hisF"
                     /locus_tag="ACF2SP_001914"
     CDS             complement(2669..3436)
                     /gene="hisF"
                     /locus_tag="ACF2SP_001914"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFD2359696.1"
                     /translation="MLTKRIIPCLDVLDNQVVKGVKFRNHRIIGDILPLAAHYSASGA
                     DELVFYDITASSDDRSVCPVWVNKVAATINIPFTVAGGIRTLHQARSILNAGADKISI
                     NSPALENPSLINELSKEFGKQCVVIGVDSQWIIDDYYVYQYTGNENKSTNSKRKTREW
                     IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVF
                     KNSHVSGALAASVFHDKILAINDIKLALQTENIEVRL"
     gene            complement(3430..4149)
                     /gene="hisA"
                     /locus_tag="ACF2SP_001915"
     CDS             complement(3430..4149)
                     /gene="hisA"
                     /locus_tag="ACF2SP_001915"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFD2359697.1"
                     /translation="MLVIPAIDLQSGRCVRLKQGRFDQVTQFSVFPIERALHFAKLGA
                     KRLHVVDLDGARSGKMQQLELICSMQKTGIPIQAGGGIRSIEQALECSNAGISQLVIG
                     SLAITNPDLTIQIIEKIKPENIVLALDVRVDTKVPLLAINGWQNNSTSSLWEVVSYYE
                     NYGIKNILCTDIACDGMMNGPNFDLYQQAVEYFPQIAWQASGGVRHMQDITTLNSLGI
                     SAVILGLMLYQDNVNFEELLC"
     gene            complement(4143..4742)
                     /gene="hisH"
                     /locus_tag="ACF2SP_001916"
     CDS             complement(4143..4742)
                     /gene="hisH"
                     /locus_tag="ACF2SP_001916"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFD2359698.1"
                     /translation="MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILP
                     GVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAE
                     LLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHE
                     EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESTC"
     gene            complement(4739..5797)
                     /gene="hisB"
                     /locus_tag="ACF2SP_001917"
     CDS             complement(4739..5797)
                     /gene="hisB"
                     /locus_tag="ACF2SP_001917"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFD2359699.1"
                     /translation="MKKILFIDRDGTLVEEPFDFQVDSLDKIKLTSGVIPALLQLQKA
                     GFTFIMVSNQNGIGTVAFPEEDFAVCHEFILDLFSSQGILFDEIFICPHTPEDNCICR
                     KPKTGLLEPYLKETAFAKHYSWVIGDRDTDKEFADNLGVNFLPISKTHTWEMVVSAII
                     NDARKASVQRKTKETAIDLSVQLDSDQTSVIDTPIPFFTHMLEQVAKHGGFDLRLQAS
                     GDLEVDEHHLIEDTAIALGEAIRTALGDKWGINRYGYTLPMDESLACVAIDISGRSFC
                     DFKGQFTREFVGGMATEMVPHFFQSLSSALGATIHIEVTGTNHHHMIEACFKVLGRAL
                     RQACSRTNNYLPSTKGVL"
     gene            complement(5775..6869)
                     /gene="hisC"
                     /locus_tag="ACF2SP_001918"
     CDS             complement(5775..6869)
                     /gene="hisC"
                     /locus_tag="ACF2SP_001918"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFD2359700.1"
                     /translation="MSILNLVRTDLLNSQNYVPGGESARYRSHANELPWSPVTMGEHN
                     LNYYPNIGLQIELQNQLAKRYQIYSDQIILTRGSDDGIDLTTRFFLTAGKDAFMQFPP
                     TFPMYAFYVRLQQAELIECPLDRRTNFRLTLDQIENSWKPNCKVIMFCSPNNPTGNLV
                     DLNLIAKTCELYANQSIIVVDEAYIEFANAPSATSLIGEFENLIVLRTLSKAFGLAGL
                     RLGCIIAQSPIIQAFNKIIAPYSIATPSMELAKRALNNSDWFTKTIEQIKSSRAWVIK
                     KFADNPIIEKIYPTETNFILIQTRFSKQLTTWLARYGIAVRDFPSSSLLHDHLRITVG
                     NDEQNQLLINALSSFNADVAGLNYEKDFIY"
     gene            complement(6870..8165)
                     /gene="hisD"
                     /locus_tag="ACF2SP_001919"
     CDS             complement(6870..8165)
                     /gene="hisD"
                     /locus_tag="ACF2SP_001919"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFD2359701.1"
                     /translation="MLTIKNWQLLSDNDKRRCLSRPRDNSAIKENVLEIINQVQLSGD
                     KALYDLTKRFDRVNLQYLQISQEKIRQASIPQNALIAIKQAIETISSYHQFLLPENTE
                     ISTASGITIRNVYRPIQKVGLYVPGGNKTPLVSSLLMQAIPAKVAGCPIKVLCTPPDA
                     EGEINEHILVAARLCGIDTIYAIGGAQAIAAMAYGTESVIKVDKIFGPGNSYVTQAKT
                     LVAIDADGAAIDMPAGPSEVMILADTEANPEFIAADLLAQAEHGPDSQVILICDECEL
                     ANQVNQQLEIQMSYLSRIEFIKQSLTNSRIIICSNQSEQLDIINSYAPEHLIINRKNP
                     EPWVEKIVAAGTVFLGSWAAETMGDYVTGSNHVLPTSGFARNHSGLSTLDFMTRFTVQ
                     AINQEAIRNLGPAAMTLAELEGLDAHANAVQIRLNTLGD"
     gene            complement(8171..9052)
                     /gene="hisG"
                     /locus_tag="ACF2SP_001920"
     CDS             complement(8171..9052)
                     /gene="hisG"
                     /locus_tag="ACF2SP_001920"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFD2359702.1"
                     /translation="MKNRLRLALQKKGRLADESLNLLQRCGLKFRIKPNALLTHVDNF
                     PIDLLFVRDDDIPTLVFDSLCDGGIVGENVLLEAFLNRPDKSFRKILTLGSCTCRLSI
                     AVPEAFDYQGSGSLDGKRIATSYPNLLNNYLAERKISAEILALSGSVEVAPRMGMADV
                     ICDLVSTGQTLEDNKLKEVDTVLNSQAVFIQTQRQLDSDMQELFAMLARRIQAVQQAQ
                     ERKYIVFHAPKAALERIAQKLPGAESPTILPLPGNNEKVAVHVVSSEGVFWNTLETIQ
                     YLGASSILVLPIEKMLE"
     gene            complement(9049..9345)
                     /locus_tag="ACF2SP_001921"
     CDS             complement(9049..9345)
                     /locus_tag="ACF2SP_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HFD2359703.1"
                     /translation="MKIHGSFKKSALPNLMQAISLLKNEDEAMQFFTDLCTPAELESM
                     ADRWQVVPLLRQGIPYRTIHEETGVSVTTITRVARCLSFGTGGYRLIAERLEKL"
     gene            9547..9717
                     /gene="cydP"
                     /locus_tag="ACF2SP_001922"
     CDS             9547..9717
                     /gene="cydP"
                     /locus_tag="ACF2SP_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase putative small subunit CydP"
                     /protein_id="HFD2359704.1"
                     /translation="MKPLTRDIVFTLSFKMILLVLLWYFCVRGMHPDLSSNKEWLLGK
                     EEQTVLTPNNKR"
     gene            9722..11254
                     /locus_tag="ACF2SP_001923"
     CDS             9722..11254
                     /locus_tag="ACF2SP_001923"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFD2359705.1"
                     /translation="MIPAAEVVDLSRLQFALTALYHFLFVPLTLGLSVMLAIMETVYV
                     MTGRELWKQMVKYWGILFGINFVLGVATGLTMEFQFGTNWAYYSHYVGDVFGAPLAIE
                     GLMAFFLEATFVGLFFFGWNRLSKLQHLTSTWLLALGTNLSALWILIANGWMQHPVGA
                     YFDYQTMRMEVSSFYEMMFNPVAQAKFVHTISAGYVTGAMFVLSISAYFLLRGRNKAF
                     AKRSMTVAVSFGLASALSVVVLGDESGYVANENQKMKLAAMEAMWHTEKAPANLTIFG
                     IPDEKSFTTKYAIDIPYALGIIATRSFNTPLQGIIELIEEGKLKIKDGMIAYDALKVL
                     QKDPKNEQAKAVFTKHSENLGYALLLKKYTENVTDATEEQINQAAHDLKPKVLPMFFS
                     FRIMVACGIYFILLFATGFYLSVRHRLEKTSWYHRVAFYSLPLPWIAAELGWIVAEYG
                     RQPWVVEGILPTFMGASSLTSGQVMTSIAGFVLFYTVLAIVEIYLMVKYIRLGPDGIN
                     HH"
     gene            11270..12406
                     /gene="cydB"
                     /locus_tag="ACF2SP_001924"
     CDS             11270..12406
                     /gene="cydB"
                     /locus_tag="ACF2SP_001924"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFD2359706.1"
                     /translation="MPLDYETLRIIWWLLIGILLIGFAIMDGFDLGVAMWLPWLGRTD
                     IERRILINSIGPTWEGNQVWFILGGGAIFAAWPTLYALSFSGFYLAMLVVLLALILRP
                     VGFKYRSKLDNPIWRAFWDWALFIGGFVPALIFGVAVGNVIQGVPFYFDESLRVFYTG
                     SFFELLNPFALGCGLLSVLMLAMHGAFFIQVKTVGTLQNKAKDRARWSALLVILLFIG
                     LGAWVYYGIDGYVLKGTVPHDGPSNPSYKDVIIQAGAWFNNYKEYPVSLLVPVLCVIS
                     AFLAIIFANKSVLAFVLSGLSVGTLIATVGVSMFPFILPSSSNPKYSLIVWDSSSSQV
                     TLFIMLVATVLLLPIVLAYTAWVYRVLRGKVTESSIKAEQDSAY"
     gene            12418..12519
                     /gene="cydX"
                     /locus_tag="ACF2SP_001925"
     CDS             12418..12519
                     /gene="cydX"
                     /locus_tag="ACF2SP_001925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFD2359707.1"
                     /translation="MWYFSWILGTGLACVFAVLNAVWLELREEDVKQ"
     gene            complement(12632..13264)
                     /gene="pyrE"
                     /locus_tag="ACF2SP_001926"
     CDS             complement(12632..13264)
                     /gene="pyrE"
                     /locus_tag="ACF2SP_001926"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFD2359708.1"
                     /translation="MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGD
                     SLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVK
                     THGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
                     TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA"
BASE COUNT         3874 a         2523 c         2696 g         4272 t
ORIGIN      
        1 atggctacag tagcttgcgc cacacaagtg agtttgagag ataaaattgg gcaaatgtta
       61 atattaggat tccagggcaa agtggttgac gagaaatcac ctattgccca ggcaattgag
      121 aaaaacaaca ttggtggtgt tattcttttt gattttaatc agcagtcaca aacttttgat
      181 aaaaatatag aaagtccgga acaagtcaac caattaaata atcagttaca aacacttacg
      241 caaagagcca accgtaaata tcatagagat aatttaccat tacttatttc tgttgattac
      301 gaggggggaa acgttaatcg attgcatccc cgttatggat tccctgaaat gccttcagca
      361 aaagacattg gctccatgtc tctggaaaga gctgatgcat cagcagaatt gatggcaaat
      421 actttaaaat caacaggatt taatttgaat tttttccctg aattagatgt gaatataaat
      481 ccagataatc ctatcatagg caaaaaagac aggagtttct cttcgatacc ggaaattgtt
      541 actcaatacg cgcaacttta tacagagcaa tttataaaac atcaaataca ttgttcttat
      601 aaacattttc ctggacatgg cagttcatta tctgattcac atttgggttt tgtagatata
      661 agtgacacct ggagcgagca agaattaatc ccttttttac aattattatc tcaacccaat
      721 cattgcgata tggtgatgat agctcatatt gtgaatagaa aactggatat gacagggcta
      781 cctgccagcc tttcttacca gattattaac ggattattgc gtcatgattt aaaatttaac
      841 ggggttgtta tcaccgatga tatgcaaatg aaagcaataa cgaattatta tggattagaa
      901 actgctgtta ctctttcaat taatgcagga gcagatatgc taatatttgg taatcaattg
      961 gtagaaaaat ttcaagactc gacagaaatc attgacatga tcgagcaaaa agttagatcg
     1021 ggagagattt ctgaacaaag gattaatgaa gcttatcaac gaattgtaaa aatgaagagg
     1081 tcttttaatt attagagatt tttctcggtt tactctgata aaagtacctg ataatctgcg
     1141 agctatagat aagctgaggt atatatgcga tgcaggtcaa ttatcaaaaa aactaatata
     1201 aaaaatgggg tttttcagat taaggtggaa ttgcatgaaa aacgcgctga aaagggaaga
     1261 tgcggcgaat aaaaaattgg gagttttgat ttatgaaaac gtccagccta tggatgttat
     1321 tgggccgtgg gaagtgtttg caacctggaa aactattctt gatgccccta ttgatatgtt
     1381 tctggtggca gaaagcagta atgaagtttg ttgcgcaaat caaattagcc tgaaacctca
     1441 ttgtgaattt aacaattctc cacaatttga ttatttactg gttccaggtg gggctggtag
     1501 aagagagcaa gtaaataatc cacgtctgat ttcttttttg aaaaagcagg ctcaaaacac
     1561 tgactatttg ctttctgttt gtactggagc atttttattg catgcggcag gcctattgat
     1621 tcatcatgaa gcaacaactt attggcgcgc tatgcctgaa ttaaagtctt ttgaggatgt
     1681 ccaattggtt gaaaagcgta tcgttaagtc aggtaaagtt tggactactg gtggaatttc
     1741 cagtggcatt gatctggcat ttgaatttat ttcaaccatt gctggcaatg atgttgcggg
     1801 aaaagtgcag ttgctttttg aatacttccc tgagtgcaag ctgtattccc agcctgctat
     1861 ggtgagtgag ttgccttctt actatgcaac taaaaaggat gaggcagtgt tggaaatgaa
     1921 tttgcctgat tacattaaag agtatttcaa gaaaagttaa tttcttaata gtagtttaaa
     1981 aaattaacta gtcttgattg ataattcctg aggattctgg ttatagagat cttctatgga
     2041 ataagcaatt aagtatgcac acttctgtct cttgcctgta aacacaataa aacatcataa
     2101 aaacttaact cacaagcttt gagaagcact aataaatgat aaattaaatc agatgcttcg
     2161 ttgataagtt cttctttatt gttgctcaca gcagcgatca ctgcttctac cgactcttct
     2221 cctaattttt gagcacatcg gttcacacca gaatccaaga gttgagcagt ataactgttt
     2281 ggattttttt cctcagcacg ttcattaatt aattcaatca aaccatcaag aaaaccaaga
     2341 gtataattat tatgtggctg atagcaggtt gaataaccta aatgacaagc gggtccttta
     2401 ggaataacct ggatcaaaag actgtcattg tcgcaatcgg cactaatatg ttgcagagcc
     2461 atactatttc cggaagactc tcccttacgc cacagtcttt tccgacttct gctgtataag
     2521 gtcagttgtc ctgtagttag tgtggcaatt aatgcttcct gattcatata acctagcatc
     2581 aatactttac cattttctgc gttttggatt attgccggca atagtccatt cattttttgc
     2641 caatcaagag agttaatgtc caagtcactc atagtcttac ctcaatattt tctgtttgta
     2701 atgctagttt tatgtcattg attgccagta ttttgtcatg aaaaacgctt gctgccaaag
     2761 ccccgctgac atgagaattt ttaaatactt caacaaaatc attaattttt ccagcaccgc
     2821 ccgatgcgat gaggggaacc tggcagaggt taatcatttc tgatagctgt gcaatatcat
     2881 atcccattcg tacgccatca gattgcatgc aattaaggac tatctcacca gcaccgcggt
     2941 cttgaatttc atatatccat tctcgagttt tgcgttttga attggtactt ttattttcat
     3001 tacctgtata ctgatagaca taataatcat caattatcca ctgactatcg acccctatta
     3061 ccacacattg ttttccaaac tctttgctca attcgttaat caatgaaggg ttttccaacg
     3121 caggactgtt gattgagatt ttatctgctc cggcatttaa tattgatctt gcttgatgca
     3181 atgttcggat acctccagcc actgtaaatg gaatattaat agtagccgcc actttattga
     3241 cccaaacagg acaaacagaa cggtcatcgc tacttgctgt aatatcataa aataccaatt
     3301 cgtctgcgcc tgatgctgag tagtgagcag ctaatgggag tatgtctcca attattctat
     3361 ggttgcgaaa ttttactccc ttgacaacct gattatccag aacgtcaaga cagggaatga
     3421 ttcgcttggt taacataata actcctcgaa attcacatta tcttgataaa gcattagccc
     3481 aaggattacg gcggatattc ctaatgagtt cagtgttgta atatcctgca tatggcgtac
     3541 tccccctgag gcttgccagg caatctgagg aaaatattca actgcttgtt gatataaatc
     3601 gaaattaggc ccattcatca tgccatcgca tgcgatgtca gtacaaagta tgttctttat
     3661 cccatagttt tcgtaataag acactacttc ccaaaggctg cttgtactat tattttgcca
     3721 gccattaatc gcaaggaggg ggacttttgt atcaacacga acgtccagag caagaacaat
     3781 attttcaggt ttaatctttt caatgatttg tattgtcaaa tcaggatttg taatagccag
     3841 gctgccaatg accaattgtg aaattcctgc gttgctgcat tccaatgcct gttctatgct
     3901 cctgattcca cctccggctt gaattggtat tcccgtcttt tgcatggaac aaatgagctc
     3961 caactgttgc atttttccgc tgcgagcacc atccaaatcg actacgtgaa ggcgtttggc
     4021 gcctagtttg gcaaaatgca aagccctttc tatgggaaaa acgctaaatt gcgttacttg
     4081 atcaaaccgt ccttgtttta agcgcacaca tcgtccagat tgcaaatcaa tagctgggat
     4141 aactaacatg tagactccaa agataaaaag ttatttagta ataccatgcc aacatcagct
     4201 gatttttctg gatgaaattg cataccataa aaattgcctt tttttacaac tgcagtaaac
     4261 tcttcatgat actgacatcg agccaatgca taatcatctg cttttagagc atagctatga
     4321 acaaaataaa cataatcact attatttaat ccttgttgta atgatgatgt tttttgccaa
     4381 tgaagattgt tccatcccat atgaggaaca ggatgatttc tttcagcttt taataactct
     4441 gcaactccag gaatcaaacc caggcagggt atgtcgtctt cttcgctgta ttcaagcaat
     4501 aattgcattc caagacagat gccaagaagt ggttgggtta aggttcttaa aacgtctatt
     4561 aacccatttt gctgcaaagc tgtcatacca gaacgtgctg ttccaacccc tggcaaaatg
     4621 acatggcttg ctttttgtat ttcttcagca tcatgggtca gtgcaaaatg ccctcccaat
     4681 cgaatcaagg catttgtcag agaagttaaa ttattcccac tgacatcaat cacagcaatc
     4741 ataatacacc cttggttgaa ggtagataat tattcgttct tgagcatgcc tgtcgcaagg
     4801 ctctgcctaa aactttgaag catgcttcga tcatgtggtg atgattggtg cctgttacct
     4861 cgatatggat agttgccccc aacgcgctcg atagcgattg gaaaaagtga ggcaccattt
     4921 ctgttgccat accaccaaca aattcacgag taaactgacc tttgaagtca caaaagcttc
     4981 tgccgctaat atcgattgca acgcaagcta atgattcatc cataggaaga gtataaccgt
     5041 aacgattaat tccccatttg tcacccagcg ctgttctaat tgcttccccc aatgcaatgg
     5101 ctgtatcctc aatcaagtgg tgctcatcta cttccaaatc tccagaagct tgtaatcgca
     5161 aatcaaagcc tccgtgtttt gccacttgct ccaacatgtg ggtaaagaag gggatagggg
     5221 tgtctataac actggtttga tcgctatcca gttgaactga caaatctatt gccgtctctt
     5281 ttgtttttct ttgcacagac gctttgcgag catcgttgat aattgcagaa accaccattt
     5341 cccaagtatg tgttttcgaa atgggcaaga aattaacgcc cagattatca gcaaactctt
     5401 tatcagtatc ccggtcgcca attacccatg aataatgttt ggcaaatgct gtttctttta
     5461 agtaaggttc gagcaagcca gttttaggtt ttctacaaat acaattgtct tcaggggtgt
     5521 gagggcatat aaaaatctca tcaaaaagaa tgccttgaga ggaaaacaga tccaggataa
     5581 attcatggca aacagcaaaa tcctcttctg gaaaagctac ggttcctatg ccattttgat
     5641 tggataccat aatgaaggta aagccagctt tttgtaattg caatagggct ggtattactc
     5701 cagaagttaa tttaatttta tccagagaat ctacttgaaa atcaaagggt tcttcaacta
     5761 aagtgccatc gcggtcaata aataaaatct ttttcataat ttaatcctgc tacatcagcg
     5821 ttaaatgagg ataaggcatt aattaataat tgattttgct catcattacc caccgtaata
     5881 cgaagatgat catgcaataa agaggacgaa ggaaaatctc ttactgcgat gccataacga
     5941 gcaagccaag tggttaattg ttttgaaaat cgtgtttgaa tgagaatgaa attggtttcc
     6001 gtaggataaa ttttttcaat gattggatta tcggcgaatt tttttataac ccaggcacga
     6061 gatgatttga tttgctcaat agtttttgtg aaccaatcgc tattattcaa agctctttta
     6121 gccagctcca tgctaggagt ggcaatggaa tagggggcaa tgattttatt aaaagcttga
     6181 ataataggac tctgggctat aatgcagcct aagcgtaatc cagccagccc aaaggctttc
     6241 gacagggttc ttaagacaat cagattttca aactctccaa ttaaggacgt agcgcttggt
     6301 gcatttgcaa actctatata cgcttcgtcc acaactataa tagattgatt cgcatatagt
     6361 tcacatgtct tggcaattaa atttaaatcc accaaattac cagtcgggtt attgggacta
     6421 caaaacatga taactttgca gtttggtttc cagctatttt caatttgatc cagggtgagg
     6481 cgaaaattag tccttctatc caaagggcat tcgatcaatt ctgcttgttg tagccgaaca
     6541 taaaaagcgt acataggaaa agtaggggga aattgcatga aagcgtcttt tccggcagtt
     6601 agaaaaaatc tggttgtcaa atcaatgcca tcatcacttc ctctggtcag tatgatttga
     6661 tcggaataga tttgataacg ttttgccaat tgattttgca gctctatttg taagccaatg
     6721 tttgggtaat aatttaaatt atgctccccc atagtaactg gtgaccaagg cagttcattg
     6781 gcatgtgatc gatagcgagc actctcacca ccggggacat aattttgact atttaataga
     6841 tctgtacgaa caagattgag tatggacatt taatccccca gagtattgag tcttatttga
     6901 acggcattgg catgagcatc caaaccctct aattccgcta aagtcattgc cgctggacct
     6961 aaattacgga tagcctcctg atttatggct tgcacggtaa atcgtgtcat aaaatcaaga
     7021 gtagaaagcc cgctatggtt tctggcaaaa ccactggtag gtaagacgtg attgcttcct
     7081 gttacatagt cacccattgt ttctgcggcc caagagccaa ggaatactgt tccggcagcc
     7141 acgattttct caacccatgg ctcggggttt tttctgttaa tgatcaggtg ttccggagcg
     7201 taggagttga taatatccag ctgttccgat tgattagaac agataattat cctgctattc
     7261 gtgagagatt gtttgataaa ttctattctt gataaataac tcatctgaat ttctagctgt
     7321 tgattaacct ggtttgccaa ttcacattca tcacaaatta aaataacctg tgaatccggg
     7381 ccatgttcag cctgggctaa taaatctgca gcaataaatt cagggtttgc ctcagtatct
     7441 gcaagaatca tcacttcaga tggcccagcg ggcatgtcaa tcgcagctcc gtcggcgtca
     7501 attgcaacta aagtttttgc ctgagtgaca tagctgttac ccggtccaaa tattttatcc
     7561 actttgatga cactttcagt gccataagcc atagccgcta tggcttgagc tccacctata
     7621 gcgtaaattg tgtcaatacc acataatctt gcagctacca ggatatgctc gttaatttcc
     7681 ccttctgcat cagggggagt acaaagcact tttattgggc agcccgctac ctttgccggt
     7741 attgcctgca tgagaagtga agaaactaaa ggtgttttat ttcctccagg cacatacaag
     7801 ccaacttttt ggattggtct gtagacattg cggattgtaa ttcctgatgc agtcgaaatc
     7861 tcggtatttt caggcaacaa aaactggtga tatgacgaga ttgtttcaat ggcttgtttt
     7921 atggcgatta aggcattttg cggtatgctg gcttgtctaa ttttttcctg tgagatctgt
     7981 aaatattgta aattgactct atcaaaccgt ttggttaaat cgtaaagtgc tttatctcct
     8041 gataattgga cctgatttat aatttccagc acattttcct ttattgcaga attgtctcgt
     8101 ggccttgaaa ggcatcgcct tttatcgtta tcagacagta attgccagtt ttttatcgtt
     8161 agcatatgaa ttactccagc atcttttcaa taggtaatac caggatggag ctggcaccta
     8221 aatattggat agtctctaaa gtattccaaa acacaccttc actggagact acgtgcacgg
     8281 caaccttttc attatttccg ggtaatggta gaatggttgg tgattcagct cctggtaatt
     8341 tttgagcaat tcgctcaagg gcggccttgg gcgcgtgaaa aacaatgtat tttctttcct
     8401 gagcttgctg gacagcctgt attcttctgg caagcatggc aaacaactct tgcatatcag
     8461 aatccaattg tctctgagtt tgaataaaga cggcctgact gttgagaaca gtatcaactt
     8521 ctttgagttt attatcttcg agtgtttggc ctgtagatac caaatcgcat atgacgtcag
     8581 ccattcccat tcgtggcgct acctcaacag agccggataa ggccagaatt tctgcactaa
     8641 tttttcgttc agcaagataa ttgttgagta aatttggata gctcgtggca attcgcttcc
     8701 catccagact cccggagcct tgatagtcga atgcctctgg tactgctatg gataaacggc
     8761 aagtacaact gcctaatgtt aatatttttc taaatgactt atccggcctg ttaagaaagg
     8821 cctccaacag gacattttct cctacgatgc caccatcaca taaactgtca aatacaagag
     8881 tagggatgtc atcatcacga acgaagagta aatcgattgg aaaattatca acatgggtta
     8941 ataatgcgtt tggcttaatg cgaaatttta aaccacaacg ttgtaggaga tttaaagact
     9001 catctgctaa tcgacctttt ttttgcaagg ccagacgtaa acgatttttc acaacttctc
     9061 caatctttca gctattaaac gataaccacc tgtaccaaag cttaaacaac gagcaactcg
     9121 cgtgatggtt gttacactga cccctgtttc ttcatgtatc gttctgtaag ggattccttg
     9181 acgtagcaaa ggaactactt gccatcgatc agccatagat tctagttcgg caggagtaca
     9241 taagtctgta aaaaactgca tggcttcgtc ttcattttta agcaagctga tggcttgcat
     9301 caaattaggc agcgcagatt ttttaaaaga tccatgtatt ttcatgttaa tgtattagca
     9361 tgttgtaaca tgttaatgat gataatgatt ctatttatca ttgtcaataa gttagtgaaa
     9421 aaaaaatctg gatagggtag agtatacagg tctctttccc ggaaactgga ttggctgaaa
     9481 atcaaaaggc agacgcaaat attttttaac agagactgtc gatatgataa atcctgagga
     9541 aatgtcatga aaccacttac cagagatatt gtatttaccc tttcatttaa aatgatattg
     9601 cttgttttat tatggtattt ctgtgtcaga ggaatgcatc ctgatctgtc tagtaataaa
     9661 gaatggttgt taggaaaaga agaacaaaca gtacttaccc caaataacaa gaggtagttt
     9721 tatgattcca gctgctgaag ttgttgattt atcaagatta caatttgcat taactgcact
     9781 atatcatttt ctatttgtgc cccttacgtt ggggttatcg gttatgttag cgatcatgga
     9841 gacagtttat gtaatgactg gccgggaact ctggaagcaa atggtcaaat actggggtat
     9901 tttgtttggg attaattttg ttttgggagt tgcaaccggt ctgactatgg aattccagtt
     9961 tggtactaac tgggcttact attcacatta tgttggagat gtatttggtg ctccattagc
    10021 aattgaagga ctaatggcct tttttctgga agctactttt gtggggttat ttttctttgg
    10081 ctggaacaga ttgtcaaaat tgcagcatct gacctctaca tggctattgg ctttaggtac
    10141 taatctttca gcattatgga ttttaatcgc taatggttgg atgcaacatc ctgtaggtgc
    10201 ctattttgat taccaaacga tgagaatgga agtcagcagt ttttacgaaa tgatgtttaa
    10261 ccctgtcgct caggctaagt ttgtgcatac tatcagtgcg ggatatgtga caggagccat
    10321 gtttgtcttg tccataagcg cctattttct tttgcgggga cgaaataaag cgtttgccaa
    10381 acgctcaatg acagtcgcag tttcttttgg tttggcatca gcattgtctg ttgtggtatt
    10441 gggagatgag agcggttacg ttgccaatga aaaccaaaaa atgaaattgg ctgcgatgga
    10501 agcgatgtgg cataccgaaa aagctcccgc caatttgact attttcggga taccagatga
    10561 gaaaagtttc acgacaaaat atgcgataga tattccttat gctcttggta ttattgctac
    10621 tcgttcgttc aatactcctt tacagggcat tattgaatta attgaggaag gaaaattaaa
    10681 aatcaaagat gggatgatcg catacgatgc attgaaagtt cttcagaaag atccaaagaa
    10741 cgaacaagcc aaggctgtct ttacaaagca cagtgaaaat ctgggttatg ctttgttgct
    10801 caagaagtat actgaaaatg taacggatgc cactgaagag caaataaatc aagcagcgca
    10861 cgatctcaaa cctaaagtac tgccaatgtt cttctcattt agaattatgg ttgcttgcgg
    10921 gatatatttt atacttcttt tcgcaaccgg tttttatttg tctgtaagac atcggttgga
    10981 aaaaacatct tggtaccaca gggttgcatt ttactctttg cctttacctt ggatagcggc
    11041 tgaattaggg tggatagtag cagaatatgg tcgccaaccc tgggttgttg aaggtattct
    11101 gcctactttt atgggagcct cctctttaac atcagggcaa gtaatgacat caattgcagg
    11161 ctttgtattg ttttatactg ttcttgcaat tgttgagatt tatttaatgg tcaaatacat
    11221 acgtcttggc ccagatggga taaatcatca ttgatgaaag gagaatcaaa tgcctttgga
    11281 ttatgaaaca ctcagaataa tttggtggtt attaataggt attttgctta ttggttttgc
    11341 cattatggat ggatttgacc tcggtgtggc aatgtggctt ccctggttag gtagaacaga
    11401 tattgagaga cggatattaa ttaacagcat tggcccaaca tgggagggta accaggtttg
    11461 gtttatactg ggcggaggcg ctatttttgc ggcttggcct actctctatg ccttatcttt
    11521 ctcaggtttt tacttggcta tgctggttgt tcttttggcg ttgattttaa gacccgttgg
    11581 ctttaaatac agatcaaagc tggataaccc catatggaga gcattttggg attgggcttt
    11641 attcatcggt ggatttgttc cggctttaat ctttggggta gcggttggaa atgtcataca
    11701 aggtgttcca ttttattttg atgagagttt aagagtattc tatacgggaa gtttttttga
    11761 attactcaat ccgtttgctt tagggtgtgg attattatct gtgcttatgc ttgctatgca
    11821 tggtgctttt tttattcaag taaaaactgt agggactttg caaaataaag caaaagacag
    11881 ggctcgctgg agtgctcttt tggttattct tttatttatt ggattagggg cttgggttta
    11941 ttatggtatt gatggttatg ttttaaaagg aacggttccc catgatgggc catcaaatcc
    12001 ttcttacaag gacgtcatta ttcaagccgg agcatggttt aataattata aagagtatcc
    12061 agtatcactt ttagtgcctg ttttatgcgt aatatccgct tttttagcga ttatttttgc
    12121 gaataaaagt gtgttggctt ttgtgctgag cggattgagt gttggtactt tgattgcaac
    12181 ggtaggggtt agtatgtttc ctttcatatt accctcttct tcaaatccta aatacagttt
    12241 gatcgtttgg gatagctctt ccagccaggt tactttgttt attatgttag tggcaacggt
    12301 attattattg cctattgtgc ttgcatacac tgcttgggtt tatcgagtgt tacgaggtaa
    12361 ggtaactgaa agttctatta aagcagagca agattctgca tattaggagg tttttctatg
    12421 tggtatttct cctggatttt gggcacagga ctagcttgtg tgttcgcggt gcttaacgca
    12481 gtatggttgg aacttagaga agaagatgtc aagcaataga cagaggcaat atcagtgtaa
    12541 gttgctgagt ttcatggata gctcagcaac tgaaaacaca atgagtgatg attgtcttac
    12601 tcttttttaa ggaaaatctg taatcctaat attatgcgcc ataacgctct tgataggact
    12661 ccagtttttg tacctgttcg tattgattgt catttttcag aaattctatt agatcaaaca
    12721 aattgataat tgaatagact tcaattcctt gctccctgat ttctgacagg gttgatttct
    12781 cagttaatcc tcgttcgcat ctgtccagag caataatgac accacgaaga atccctccat
    12841 tctctttaat cagggtttgt gattctctga atgctgtgcc cgctgtaatc acatcgtcaa
    12901 tgatcactgt ccttcctgtt agcggcgaac caattaattg tccgccttct ccatgagttt
    12961 ttacttcttt acgattaaaa gtcactgtga tgtccctgcc taactcagcc aaggccactg
    13021 cagttgcggt agcgagtggg atacctttgt acgctggtcc aaacaagtgc tcaaaagaaa
    13081 cttcttgttc taaaagggtt tttgcataaa attgacctaa cttacgtaag gagtcaccgt
    13141 gataaaataa accagcgtta aagaaatagg gacttattcg tcctgatttt aaagtaaatt
    13201 caccaaattt aagtacttga cactccagag ctaattttat aaaagatgat ttggtatgat
    13261 tcattctggt ttcctaatac aaaattttat tgaaaacaat cacatatttt aaaaaaatcc
    13321 ttaacaatca acaaaaaact gatatgctaa tcatagtgca ggatt
//
LOCUS       DAXLDT010000083        13364 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXLDT010000083 DAXLDT010000000
VERSION     DAXLDT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.083"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1125
                     /locus_tag="ACF2SP_001927"
     CDS             <1..1125
                     /locus_tag="ACF2SP_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFD2359709.1"
                     /translation="ARFINQQLLIDGPEKEKIVKNYKRLTLCFHPDHASGFSPEMVWL
                     ENNLSQSKNNGACFKILGLCYEKLISPEKFKDSSLGDIKSKDDFKQWLENLRSKATTY
                     SGQSFCDSLINLLEQSSGFFDDTGKIKPRGLRVLLRFIPVVFASYGTFLFAEELFAIY
                     ALYFILLKGGQYLESTESSQLKKIGCTLQEISIITATASTTLLVRLLEMTFWASRQFL
                     DVSLQIGSAILKPMLPSPETKEKSESDTAANLCRDLILASHNTGEGMQFKTPELKVIS
                     APLESYLGLNAQQFFGDLRIGREKRLKVEAFLFKMRVLDSLPSPLEEKLVEAQKELDK
                     IKKDTKVFTSNTAKAVNEAEQVISLLQDQDSMQLVVYNGT"
     gene            1534..2283
                     /locus_tag="ACF2SP_001928"
     CDS             1534..2283
                     /locus_tag="ACF2SP_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFD2359710.1"
                     /translation="MKILVAVKRVIDPYVKIRVKSDNSGVETQNIKMAMNPFDEIAIE
                     EALRLREKNWATEVIAVSIGSDSSQETLRHALALGADKAILVRTEKSFESLNIAKILN
                     SIVANEKPDLVLMGKQSIDGDNNQTPQMLAALLNWPQATNASKIIPDNNNLEVVREID
                     GGLETLKVQLPAVISTDLRLNEPRYASLPNIMKAKRKPLDIIDLDSLGLSLKQHVEIL
                     KVNAPATRSGGVILDSVAALLDKLQHEAKVL"
     gene            2297..3235
                     /locus_tag="ACF2SP_001929"
     CDS             2297..3235
                     /locus_tag="ACF2SP_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFD2359711.1"
                     /translation="MSTLVLVEHNNQQLHPATRNTLSAALELGDKITLLVVGYQCRSV
                     AEEAASLEGVHAVLHVDNPCYEHQLAEAVSDLVVSFANSFSAILAPSSTYGKNILPRV
                     AAQLDVTQISDVSKIINSDTFEHPVYAGNAIETVRVLDSLKVMTIRTTAFDPITSSQE
                     VCCIESIDKEFQAKGSQFVRHELSKSERPDLGSAKIVVSGGRGLQNAEKFKLIEELAD
                     TLGAAVGASRAAVDAGFAPNDYQVGQTGKVVAPMLYIAVGISGAVQHLAGMKDSKVIV
                     AINKDADAPIFQIATYGLVGDLFELVPQLIEHLKNR"
     gene            3247..4368
                     /gene="ald"
                     /locus_tag="ACF2SP_001930"
     CDS             3247..4368
                     /gene="ald"
                     /locus_tag="ACF2SP_001930"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HFD2359712.1"
                     /translation="MLVGVPKEIKSHENRVGLVPSSVREIIRAGSEVLVEKGAGLGIG
                     ISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPH
                     QARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
                     GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTV
                     YSTADSIEKYVSSADLVIGAVLVPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFE
                     TSRATTHQEPTYVVDGVVHYCVANMPGAVPRTSTFALNNATLPFILSIVSKGVKLALL
                     SDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR"
     gene            complement(4440..6824)
                     /locus_tag="ACF2SP_001931"
     CDS             complement(4440..6824)
                     /locus_tag="ACF2SP_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFD2359713.1"
                     /translation="MKKMAYFWRKGLWALMSLFFLLIVAGSLLYLYLESQLPNVDSLK
                     TVQLQVPLQIFSKEGLLIQEYGEKKRIPVTYDEIPPTLIHALIATEDQRFFEHPGVDV
                     MGLGRAAVSMLKTGTKSQGGSTITMQVARNFFLSRKKTFLRKFNEIMLAIKIDRELSK
                     EKILELYLNRVYLGNRAYGVGAAAMVYFGKSLKDLNLAELAMIAGLPQAPSTQNPIAN
                     PLAAKKRRDHVLERLLEEHYINEEQYQNAVNQPITAKYHGTNIKVKAPYVAEMIRQSL
                     YDNFGPEAYTKGYKVYTTIDGKLQNTANEVVEKNLIAYDHRHGYRGPIANIGEKDSQS
                     PKIRQKYLRQYPELNTLIPAVVTEVREKEATAALQNGQIIIIPWEGMSWARPALKRGG
                     WVGKSPTKAQQVVAAGDIIYVHPTEKSWQLAQIPEAESAMVAMNPKNGAIEVLVGGFN
                     FSKSKFNRATQSSRQPGSSFKPFVYAAALNNGYNLATLINDAPIVVDDPSQPNLWRPH
                     NVNLKFNGPTRLKQALVQSKNLVSIRILDDIGIDYTIDFLTRFGFNKKSLPRALSLAL
                     GSLSISPMDLTTAYAVFANGGYKVEPYLIDHITDADGKILLQAKPSVVCDPCDKDKVD
                     ASTLAPRVISEDIAFLMNTALRDVIQHGTGRAARVLNRQDIAGKTGTTNDQVDAWFAG
                     FNPNLVVTTWIGFDNPKSLHEYAANLALPLWIDFMKVALKDKPETELKQPENVIAVRI
                     DPVSGLLAKPNQENGIIEYFRQNEVPSEEDQGPVYNAHNDKQEEELAPVEESLF"
     gene            complement(6974..7981)
                     /locus_tag="ACF2SP_001932"
     CDS             complement(6974..7981)
                     /locus_tag="ACF2SP_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359714.1"
                     /translation="MTRLEELLYTLTSVIIRYHDSQPKVLNKLIVESNPILLRRKALA
                     LAKEIIQDKDVECKGRLEELIQNCTKGYPDRKPFLYFILNEVLFLKSMLDKKSSFEPS
                     KLEEYKKQITQLLIDLKQLMITPKSKTYEVKYTSATENSETSISLSGVKNDGYVGGEF
                     CNSGNLIKEEVLEALNIHINSKNTAECSSDEEIKALAESLCLEHQNALLVPELLEKAR
                     ILTESSQEQESELLTKTEQTKTDKKIILEQQATIQEKDKEIEVLKEKVSELTRSMPRP
                     VFNPVLPLGYGSLFFNTKLGTQAKQQTMLDVTDLSIAKPSESKDAEPNKSFSMGNSHG
                     D"
     gene            8398..9462
                     /gene="pilM"
                     /locus_tag="ACF2SP_001933"
     CDS             8398..9462
                     /gene="pilM"
                     /locus_tag="ACF2SP_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HFD2359715.1"
                     /translation="MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEI
                     PASALEGNMIKDVDVVANCIKRLVERLKFTSKNVALAVPDSAVISKVVQINEGLTDLE
                     MEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
                     AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFS
                     REEKFGGMQLVEAIAEHYHMNLEQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTL
                     QFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANPLSHMSFGKKVNLDV
                     INSDAPTLMVACGLALRHVE"
     gene            9466..10014
                     /locus_tag="ACF2SP_001934"
     CDS             9466..10014
                     /locus_tag="ACF2SP_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFD2359716.1"
                     /translation="MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVS
                     HLISNQTNRNQILQKEITALDEQIKEIKALKQIREGLISRMSIVQNLQSTRTLMVHLF
                     DELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
                     NVEKKQSPNNEFKLSFVLKPKY"
     gene            10028..10627
                     /locus_tag="ACF2SP_001935"
     CDS             10028..10627
                     /locus_tag="ACF2SP_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFD2359717.1"
                     /translation="MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKP
                     NFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQLQLMNERFGTMLKQLPAKNEMPG
                     LLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
                     IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ"
     gene            10624..11208
                     /locus_tag="ACF2SP_001936"
     CDS             10624..11208
                     /locus_tag="ACF2SP_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFD2359718.1"
                     /translation="MIIKNNQTLILGFFGNSLCTFLTSENFRKSLLTALLVLLLSACS
                     GDNSDLVKYINEVKSRPGRPIEPIPKFAPLPIFRFPENDDRRNPFKPIDQKKRNDIYA
                     PDKKRPKQPLESFPLDALKFVGTLKKDNEIWALIRQPDGQISRVRVGDYMGQNYGRII
                     LIKNDLIKLEETVQKSGTWEKQSTTINLDTGKQE"
     gene            11212..13308
                     /gene="pilQ"
                     /locus_tag="ACF2SP_001937"
     CDS             11212..13308
                     /gene="pilQ"
                     /locus_tag="ACF2SP_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFD2359719.1"
                     /translation="MRKIIAILILISASLSLAYSKDNSLISVQVLPLPENRVRIDFQF
                     SKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRA
                     ILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
                     EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITM
                     QQEGKKVRMTILSKGDYGHFVYQVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLN
                     FQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQALDIILTTQGLDKRRK
                     GNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSL
                     LSDRGKISVDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQD
                     IGIRWGVSRPTHLSGTLEGANELARGTAPANVVPLERRLNLDLAAAPLTGATPASVGI
                     ALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQEATSSGA
                     TAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVN
                     NGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITN
                     ALSITTIEGREKDVYK"
BASE COUNT         4103 a         2305 c         2845 g         4111 t
ORIGIN      
        1 gcaagattta ttaaccagca attattgata gatgggccag aaaaagaaaa aattgttaaa
       61 aattataaaa gattaactct ctgctttcat cctgatcatg cttcaggatt ttcgccagaa
      121 atggtttggc tagagaataa tttatctcag agcaagaata atggagcatg cttcaagata
      181 cttggcttgt gttatgaaaa attaatctca cctgaaaaat tcaaagatag tagtttagga
      241 gatatcaaaa gcaaggatga ttttaaacaa tggctggaaa atttaagatc caaggcgact
      301 acctattctg gccagagttt ttgtgacagt ttaattaatt tattagaaca atccagtggt
      361 ttttttgatg atactggcaa aataaaacct agaggactca gagttttact tcgatttatt
      421 cctgttgtat ttgccagcta tggcactttt ctttttgcag aagaattgtt tgctatctat
      481 gcgctctatt ttattttatt gaaaggaggg cagtacctgg agagcactga atccagtcaa
      541 ttaaaaaaaa taggctgcac gttgcaagaa atcagtatta ttactgcaac tgcatcaact
      601 actctattag ttcgcctgct tgaaatgact ttttgggctt cgcgccaatt tcttgatgtg
      661 agtttacaaa tcggctctgc gattttaaaa cctatgttac cctctcctga gactaaggaa
      721 aaatcagagt ctgatacagc tgccaatttg tgtagagatt taatcttggc tagccataat
      781 actggtgagg gaatgcaatt caagacaccg gagttaaaag tcatttctgc tcctcttgaa
      841 tcttatcttg gtttaaacgc gcaacaattt tttggtgact tgaggatagg tcgagagaaa
      901 cgtttaaaag tagaggcatt tttattcaag atgagagtac tggattctct tcctagtcct
      961 ttggaggaga aattggtaga agctcaaaaa gaactggata agataaagaa ggatactaag
     1021 gtttttacaa gcaatacagc aaaagctgta aatgaagcag aacaagttat ctctctgctg
     1081 caagaccagg attctatgca attggtggtt tataatggga cttaatagct gttgcaaata
     1141 tctcatgctt attctcaggg caaaatcaca cattggttaa ataacgaatt atgtgtgcga
     1201 ttggtgcgta tgagtgaagc tattgcaaat cccgatcgtc agtttgctat aaaaacagct
     1261 tccctaccta tgcgtgaatg acaaaatata accattgttt gcactgttga ttgtgcttaa
     1321 gggcattatt ttgccctatg ttttttcttt ttgcatgaat tgagttttcc aactcggacc
     1381 agattattag gcaggtgaga atagaaaaag gttttcttta acatagctca tttagaataa
     1441 attagcttta tgttctaatt aaaatcaacc tcttgaattt atacccctat ctctggcaag
     1501 atgcagactg atttgatatt ccagtgagga accatgaaaa tactcgtggc tgtaaaacgt
     1561 gtgattgacc cttatgtcaa aattcgagtg aaatcggata attcaggagt tgaaactcaa
     1621 aatatcaaaa tggcaatgaa cccatttgat gaaatagcca ttgaagaggc attgcgtttg
     1681 cgagaaaaaa attgggcaac ggaagttatt gctgtaagca ttggttcgga tagctctcag
     1741 gaaactttaa ggcacgcttt agctttgggt gcggataagg ccattctggt tcgaacagaa
     1801 aaatcattcg agagccttaa tatagccaag atattaaata gtatcgtagc taatgaaaaa
     1861 ccagatttag ttttgatggg caaacaatcg atagatggtg acaataatca aacccctcaa
     1921 atgttggctg cattattgaa ttggccacag gctaccaatg cgtcgaagat cattcccgat
     1981 aacaataatc ttgaagtggt gagagaaatt gatggtggtt tggaaacact caaggtacag
     2041 ctgcctgcag taatcagtac cgatcttcgt ttaaatgagc ccagatatgc cagcttgccg
     2101 aatattatga aggccaaacg aaagcctctt gatatcattg atctggattc gcttggctta
     2161 tctcttaaac aacacgttga aatattaaag gttaatgccc cggcaacgag aagtggtggt
     2221 gtcatacttg attctgtggc tgcattgttg gataaattac aacatgaagc caaagtgctt
     2281 taattaagga gatgagatga gcactttagt tttagttgaa cataataatc agcagttaca
     2341 tccagcaacc cgcaatacac tgtctgctgc tttagagctc ggtgataaaa ttacattact
     2401 agtggttgga tatcaatgcc gttcagtagc cgaggaggca gccagtctgg aaggagttca
     2461 tgctgttttg catgtagata atccttgtta cgaacatcaa ttggctgaag cagttagcga
     2521 tttggtggtg tcttttgcga attcattcag tgctatttta gctccttcca gtacttatgg
     2581 gaaaaatata ttgcccagag ttgctgcaca acttgatgtg acccagattt ctgatgtgag
     2641 caagataatt aattcagaca catttgaaca tcctgtttat gctggtaatg ccatagagac
     2701 tgtgcgtgtt ttggattcat taaaagtaat gactattaga acgacagctt ttgatcccat
     2761 cacatcaagc caggaagtat gttgtataga aagtattgat aaggaatttc aagccaaagg
     2821 aagtcaattt gtgcgccatg aattaagtaa atccgagagg ccggatttag gtagtgctaa
     2881 aatagttgtt tctggcggta gagggctaca aaatgcagaa aaatttaaat taatcgaaga
     2941 gttagctgat actttgggcg cagcagtagg ggcatccaga gctgcagttg atgctggttt
     3001 tgctcctaat gattatcagg taggtcaaac cgggaaggta gtggctccta tgctttatat
     3061 tgccgtgggt atttctggag cagttcaaca tttggcaggt atgaaggatt ccaaggttat
     3121 tgttgcaatt aataaagacg cagatgcacc tatttttcaa attgcgactt atgggttagt
     3181 aggtgatctg tttgaattag ttccacaatt aatagaacat ttaaagaatc gataagaggt
     3241 aacaatatgt tagtaggggt accaaaagaa atcaaatcgc acgaaaacag agtaggactt
     3301 gttccctcga gtgttagaga aattattagg gcaggaagtg aagtattggt agagaaggga
     3361 gctgggcttg gtattggcat atcggatgat gattacagaa atgccggggc tgaagtagtt
     3421 gatactgctg atgacgtatt tgccaatgct gaattaatag tcaaagtaaa agagcctcag
     3481 cctattgaat gtaaacggtt acgtgaaggg caaacattat tcacttattt acatttggct
     3541 cctgatcctc atcaagcgcg tttattaaaa gaatccggag ttacagcgat agcttatgaa
     3601 actgtaacac aggatgatgg cgggctacct ttacttactc ctatgtccca agtggctggt
     3661 cgtatggcaa tccaggccgg tgcacattgc ttggagatgg ctcaaggggg tagcggtata
     3721 ttactaggtg gtgtacctgg tgttgctgca gccaatgtcg tagtcattgg cgggggcgtt
     3781 gtaggaacaa atgctgttcg catggcgatg gggatggaag ctcgtgttat tgtgctggat
     3841 cgttcactgc aacgtttaaa tgaattggat tttcagtttg gttctaaact caatacagtt
     3901 tactcgactg cagattccat agaaaaatac gtatccagcg ctgatttagt gattggtgct
     3961 gtattggtac caggtgctgc agccccaaaa ttagtgactc gctctatgct gagctcaatg
     4021 cgtcctggtt ctgttttagt cgacgttgct attgatcagg gaggctgttt tgagacaagt
     4081 cgtgcgacaa cccatcaaga acctacttac gtggtcgatg gcgtagtgca ttattgtgtg
     4141 gccaatatgc ctggtgctgt acccagaaca tcaacgtttg ctttaaataa tgccacatta
     4201 cctttcattt taagtattgt ttccaaaggg gttaagctgg ctttattgag tgacccgcat
     4261 ttacttaatg gtttaaatgt tcaccagggt aaaatcactt atgaggctgt tgctcgtgat
     4321 ttaggatatg aatacacacc agctgcagct gctttatcag gacgataatt gttagttaaa
     4381 acccgtatta caaaaattga atacgggttt tgggcttcat cagaatataa atcttcaaat
     4441 taaaacaaac tttcttccac tggcgctagt tcttcttcct gcttatcatt atgtgcatta
     4501 tagacaggac cttgatcctc ttcagatgga acctcatttt gtcgaaaata ctcaatgata
     4561 ccattttcct ggttaggctt ggcgagcaaa ccgcttactg gatctattcg aaccgctata
     4621 acattttcag gctgcttcaa ttcagtttca ggtttgtctt ttaatgctac tttcataaaa
     4681 tcaatccata gcggaagagc aagatttgca gcatattcat gcaatgattt gggattatca
     4741 aaaccaatcc aggttgttac taccaaatta gggttaaatc cggcaaacca cgcatctact
     4801 tgatcattag tagtccctgt tttaccagcg atgtcttgac gatttaatac tcgtgcagca
     4861 cgtccagtgc catgctgtat tacatcgcgt aatgccgtat tcattaaaaa ggcaatgtct
     4921 tcagaaataa cgcgcggtgc caaggtagag gcatcaactt tgtctttatc acaagggtca
     4981 catacaacac ttggtttggc ttgtaacaga attttaccat cagcatctgt tatatggtca
     5041 atgaggtagg gctctacttt ataaccacca ttagcaaaga ctgcataagc agtggttaaa
     5101 tccatagggc taatggacaa acttcctaaa gcaagtgata aggctctagg caatgatttt
     5161 ttattaaagc caaaacgagt taagaaatct atcgtataat caataccaat atcatcgaga
     5221 attcttatag ataccaaatt tttagattga actaatgcct gtttcaggcg ggtagggcca
     5281 ttgaatttta gattcacatt gtgtggccgc cataaattcg gttggctggg atcgtctaca
     5341 acgatggggg catcattaat taaggtagca agattataac cattgttcaa agctgcagcg
     5401 taaacaaatg gtttgaaact ggagcctggt tgcctgctgg attgggtagc tcggttgaat
     5461 ttactttttg agaagttaaa tcctccgacc aaaacttcaa tggcgccatt tttaggattc
     5521 atagcaacca ttgcagattc tgcttcagga atttgggcga gttgccagga tttttccgtg
     5581 gggtgtacat aaattatatc acctgctgct accacttgtt gtgctttggt tggtgatttc
     5641 ccaacccagc ctcctctttt cagtgctggc ctggcccagg acataccctc ccagggaata
     5701 ataattattt gcccgttttg taatgccgca gtcgcttctt tttctcttac ttcagtgacc
     5761 actgctggaa tcagggtgtt cagctctgga tattgccgca agtatttttg cctaatcttg
     5821 ggagattgac tgtctttttc acctatattg gcaattggcc ctctgtaacc atgtcgatga
     5881 tcgtaagcaa taagattttt ttctaccact tcattggcag tattttgtaa tttgccatca
     5941 atggttgtat ataccttata gcctttagtg tacgcttcgg ggccaaaatt gtcatacaga
     6001 gactgacgaa tcatttcagc aacataaggc gctttcacct tgatattagt tccatggtat
     6061 tttgcagtaa ttggttgatt gactgcattt tggtattgtt cttcattgat ataatgttct
     6121 tccagtaatc gctccagtac atggtcacga cgtttttttg cagcaagcgg gttagcgata
     6181 ggattctgag ttgagggagc ttgaggaagt cctgctatca tagccagttc agccagatta
     6241 aggtctttaa gggattttcc aaaataaacc atggctgccg cgccaacacc ataagctcga
     6301 ttacccagat aaaccctgtt taagtaaagt tccaaaatct tttctttact taattcccgg
     6361 tcaattttga tagcaagcat aatttcattg aatttccgta aaaatgtttt tttacggctt
     6421 aagaaaaaat tacgagctac ctgcatggta atcgtactgc caccttgaga tttggttcca
     6481 gttttgagca tgcttactgc ggctcgaccc aatcccataa cgtctacacc agggtgctca
     6541 aaaaatcgtt gatcttctgt tgcaattaat gcatggatca gagtaggggg aatttcgtca
     6601 taagttacgg gaatacgttt tttttcgccg tattcctgaa ttaataaacc ttctttgctg
     6661 aaaatctgta atggtacttg taattgtacg gtttttaatg aatcaacatt aggcaattgg
     6721 ctttcgagat aaaggtacag aaggctgcca gccactatta ggagaaaaaa caggctcatc
     6781 agcgcccaaa gacctttacg ccagaagtat gccatttttt tcatagaatt tcgttttcgt
     6841 taaatgtgag tagtgcaata ttatacagcg aatctatata atattgcgag atttataggg
     6901 aaaaaaccgg atttaaatgc tattttgcgc ctcacagcta tggatataat cattgatgaa
     6961 gcgccaatag atattaatcc ccatggctat tacccataga aaatgatttg ttaggctctg
     7021 cgtcttttga ttcagagggc ttagctattg ataaatcggt cacatcgagc attgtttgtt
     7081 gtttggcttg agtgcccagc ttggtattga aaaaaagaga gccgtatcct agaggcagga
     7141 cagggttgaa aactggacga ggcatgctgc gagttagttc gcttactttc tctttcagta
     7201 cttctatttc tttgtctttc tcctgaattg tagcttgttg ttctaaaatt atttttttat
     7261 ctgttttagt ttgttctgtt tttgttagca gttcgctttc ctgttcttgg ctgctttcag
     7321 tcaatattcg ggccttctcc aatagttcag gtaccaggag cgcattttga tgttctaagc
     7381 ataaagattc agcaagcgct ttaatttctt catcagaaga gcattctgct gtatttttag
     7441 aattaatatg aatgttcaat gcttctaaaa cttcttcttt gatcaaattt ccggagttgc
     7501 aaaattctcc gccaacgtat ccatcatttt tcacacctga aagagaaatg ctggtttcag
     7561 agttctctgt agcagaagta tattttactt cataagtttt acttttaggg gtgatcatta
     7621 attgtttgag atcaattaaa agctgagtga tctgtttttt atattcttcc agtttagatg
     7681 gctcaaatga gcttttctta tccagcatgg attttagaaa aagaacttca tttaaaataa
     7741 aatacaagaa tggttttctg tcagggtagc ctttggtgca attttgtatt aactcttcaa
     7801 ggcgtccctt acattcaacg tctttatcct gaataatttc tttagccaaa gcaagtgctt
     7861 ttcttcttaa gagaatggga ttgctttcaa ctattaattt atttaatact ttaggctggc
     7921 tgtcatgata ccgaatgatt acagaagtca gcgtatagag tagctcttct aatctagtca
     7981 tgtgtaaagc tcctttttat aaagctaaaa atctaaatgt ggaaatcccc tacaacttgg
     8041 tatttcacta tattgaatgt tttgcataag tgcaagtgcc tattaactgg tacattattt
     8101 gaaaaaaaca cacaagtaga ggggttgttt tacagttttt tgaaagaata taaaatctga
     8161 tttgattgta atactatgat tgatatcatg aaatttgctt gggcatcgac agaaggcaga
     8221 aatagaaatg catggatcaa ttttcagttt gattataaac gcttaaccca aagtaataag
     8281 taaagtgatt ccaatttata gtggcttacg ctaacattaa ttattcaata gtgaggaaaa
     8341 gatctagaaa taggagtgct tttgggctgt aaggaaatat acagggagat tatataagtg
     8401 ctcaaattgc ttaaacctaa gcatcgttca attcttggca ttgacatcag ttcttccgca
     8461 gtgaaaattc tggaaatatc aggttccgga gaggacattt gtgttgaagg atatggtcgt
     8521 gaagaaatac ctgcctctgc cctagaagga aatatgatca aggatgtcga tgtagtagcg
     8581 aattgtatca aaagattagt agaaagattg aagtttacat ctaaaaatgt ggcattggct
     8641 gtacctgatt cggctgttat cagtaaagta gtgcaaatta atgaagggtt aactgacctt
     8701 gaaatggaag agcttgtcgt tattgaggcg gataagtaca ttccttaccc aatagatgaa
     8761 ataaacctgg attttgaagt gttaggccat tcagccaaga gctcatcact gcttgatgtt
     8821 ttaatcgtcg catcaagggc agaaaacgtt aatacccgtg ttgaagcagc ccatagagca
     8881 gggctggaac ccaaagttgt tgacgttgaa tcttatgcag tagagcgttc agtacaacaa
     8941 attgcgcaag aattaccagc aagtgggcaa gacaaaattg ttgcaatagt agatattgga
     9001 gccaattata ctcacttatt tgttttgcat ggtatgcgac taatcttttc aagagaagaa
     9061 aaatttggag gtatgcaact tgttgaagct attgcagaac actatcatat gaatttggag
     9121 caggctattg ccctaaaaaa tcagggtaaa ctaccggagg actacgaatc tgctgtatta
     9181 gaacctttta aggacatgat tcttttgcag atcaaacgta ctttgcagtt tttttattca
     9241 accagtcaac atggatttgt agaccatatt ttattggcgg gaggaatggc aagacaacca
     9301 ggattggcag cgcttattca agaacagtta ggagtgacta ctacagtggc aaatccacta
     9361 tcccatatgt cttttggtaa aaaagtgaat ttggatgtga ttaacagtga tgcacctaca
     9421 ctgatggtgg cttgtgggtt ggctttgagg catgtcgagt aaaaaatgac tgaaattaat
     9481 ttattgccat ggcgtgaaca aaaacgtgag caggaaaaga aattatttac aaccatgcta
     9541 ttgataggga ttatcatcgc tgcggcaatt gtttttttaa tgaactctta tgtctctcat
     9601 ttaataagta atcaaacgaa ccgaaatcag attttacaaa aagaaattac agctttggat
     9661 gaacagatca aggagattaa agcattaaaa caaatcaggg aaggtttgat ttcaagaatg
     9721 tcgattgtgc agaatctgca gtcaacaaga actttaatgg ttcacttatt tgatgagtta
     9781 ataaaaatta ctcctccagg agtttacata accaagatgc aaagagaaca cgatgtggta
     9841 acactttggg ggtattccga atctaataca agtatttcta ttttgatgag aaatatagag
     9901 gccaatgtct ggatacagaa tcctgttcta actgaaatta aaaaaaatgt tgaaaaaaaa
     9961 cagtctccaa acaatgaatt taaattaagt tttgtgctta aacccaaata ttaggctgat
    10021 aaaaatcatg gacacggtca atttaaatga attaactcta gagaatgtcg ggcagtggcc
    10081 aatgccggtt aaaatcggag taattttagg catcagtata ctgataattg gattaggata
    10141 ttggcttatt gttaagccca attttgagca attggaaata caggaagccc aggaaattgc
    10201 tttgaaaaca gagtttgaaa aaaagcaaca tcaggcagca aatctacaag cttatcgtaa
    10261 tcagttacag ctaatgaatg aacgctttgg gactatgcta aaacaactcc cggcgaaaaa
    10321 tgaaatgcct gggcttttgg aggaaatatc taaaacgggg atcgcatcgg gtttgaaatt
    10381 tgaattgttt gcaccacaac ctgaagtagt gcatgatttt tatgtggagt tacctataaa
    10441 aattacgatt gtcggatcct atatgcaatt ggcaagattt ttaagtcgtg tagctgaaat
    10501 gaacagaata gtgaccttgc atgaatttag tgttgagggg gtatcatccg gggatggtaa
    10561 aacggtctct caggatgaat tggtgatggc catcactgcg aaaatataca gatatcgggc
    10621 acaatgataa ttaaaaataa ccagactctt atattaggct ttttcggaaa ttctctgtgt
    10681 actttcctca cctcagagaa tttccgaaaa agcctattga cggcgttatt ggttctatta
    10741 cttagtgcct gctctggtga taacagtgac cttgttaagt acatcaacga agtgaaatca
    10801 agaccaggaa gacctatcga gcctattccc aaattcgccc ctttgccaat ttttagattt
    10861 ccagaaaacg atgataggcg aaatccattc aaacctattg atcaaaaaaa gagaaatgat
    10921 atttatgccc cagataaaaa aaggcctaag cagcctttgg aatcttttcc tctggatgct
    10981 ttaaaatttg taggtacttt aaaaaaagac aatgaaatct gggctctgat taggcaacct
    11041 gacggtcaga ttagccgtgt tagggttgga gattatatgg gacaaaatta tggacgtatt
    11101 attttgatta aaaatgatct cattaaattg gaagaaactg tacagaaatc aggaacgtgg
    11161 gaaaaacagt ccaccacaat taatttggat actggcaagc aggagtgagg cgtgagaaaa
    11221 attatcgcga ttttgatatt aattagtgcc agtttgagcc tggcatattc taaagataat
    11281 tcgctcattt cagtacaagt attgccattg cccgaaaaca gagtacgtat tgattttcaa
    11341 ttttccaagc ccttaaagca gttgcccgca agctttatta ctcaaaagcc gcctcgtttg
    11401 gtgttagatt ttatcagcac tgatatgcaa attccttcgg atcagaaaac taaaactatt
    11461 caattaggct cattacacag ttataacatt gttgctgttg gtgatagagt aagagccata
    11521 ttagaactgg atcgttcagt gtcctattct ggcagtacgg ctggttccgt ttatagcctt
    11581 gtcctgaatg gtaaaagcaa tgatttattt gaaaatagca aggaggtatt cataacgaac
    11641 caggttgtga acgccaagca tgaaattaaa agaattgatt ttcgtggtat agaaaagcag
    11701 ggaggtagag ttttaattga tgtttctgat acgggtattc caattgatgt aactcaagtt
    11761 gggaaagaaa tagtcgttaa tttcttgaat accaagatcc ctttgaattt aatgaaacgg
    11821 tatgatgttt ctgattttca cagccctgtg cagattatta cgatgcaaca ggaaggcaaa
    11881 aaggttcgca tgaccatcct cagcaaaggg gattatgggc attttgttta tcaggtcaat
    11941 aaacaattta tggtcgatgt ctttccttta actgcggaag aaattcgtca ggcgaaattg
    12001 aagaaacagg tatttaccgg taaacggatt tctttgaatt tccagaatat tagtattcga
    12061 gctgtgcttc aacttctggc agattttacc ggaatcaata tggttgtcag tgacaaagtg
    12121 caaggagata ttacgcttag attaaatgat atcccctggg atcaggctct tgatattatt
    12181 ttaaccacac aagggctaga taagcgcaga aagggcaacg tcatgttgat agataccaaa
    12241 gcctccatgg ataaaatgga ggaagaacaa ctcaaaagtc aacagacaat tgaaaaactt
    12301 gagcctatcc gctctgattt aatacagatt aattatgcaa aggcagcaga tattgctgtt
    12361 ttgatcaagg ataagcaaaa ctccctatta tcagacagag gaaaaataag tgttgatgcc
    12421 agaacgaata caatctggat tcaggatact ggcaccaaaa ttgaggaagt aagggaactg
    12481 ataaagcaat tggatatccc tgttaagcaa gtgcttattg aggcaagaat tgttgaagta
    12541 actaaagatt ttagccagga tattggaata cgctgggggg tatcaagacc aactcactta
    12601 agtggtacac tggaaggtgc taatgagtta gcgagaggca cagcaccagc caatgttgtc
    12661 cccttggaaa gaaggttaaa tcttgatttg gcagctgctc ctttaactgg agctacaccg
    12721 gcctcggtag gtattgcgct agctaaactt aatgataaca ttctgcttga tctggagttg
    12781 tctgctttgg aaagcgaagg gcgtgctgaa ttaatatcca gtcctcgcct gattactacg
    12841 aatcaacagg cagcagtcat agagtctggt gaggagattc cctatcagga ggcaacatca
    12901 agtggagcga ctgctgttgc atttaaaaaa gcagtattga gtttgaaagt tacgccgcaa
    12961 attacgcctg atggtaaaat tttgatggat ttacagataa atcaggatac tccttcccca
    13021 caaacattcc ttggtgttcc agcgataata acaaaggaga ttcagacgaa tgttctggtt
    13081 aataatggac aaaccatagt acttggaggt atttataagc aagataagaa caaggtaatc
    13141 aatagaattc ctttctttgg tgagttgcct gttgtcggta ttttatttag taatagacaa
    13201 atcacattga aaaatgaaga gttacttatt tttattacac caagaataat aacgaatgcc
    13261 ttatcgatta caacgataga gggacgtgag aaagatgttt ataaataatt aatattggac
    13321 taattttatc tcattaataa agataaaaaa cattaattta atag
//
LOCUS       DAXLDT010000084        13334 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXLDT010000084 DAXLDT010000000
VERSION     DAXLDT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.084"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..325
                     /locus_tag="ACF2SP_001938"
     CDS             <1..325
                     /locus_tag="ACF2SP_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359720.1"
                     /translation="NKTFITDGLATCAAVAIYGSDSGCSAAFTHMSSEASDKDDKRKE
                     TILNEMMEYVLKSNSLSDVKLIISPSRIQEKHLISFILEWVKQKKIACSMLETGGGFS
                     SIQYR"
     gene            342..551
                     /locus_tag="ACF2SP_001939"
     CDS             342..551
                     /locus_tag="ACF2SP_001939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359721.1"
                     /translation="MLSTSLQLKQDVSDKTWHGHGIIISQSEGIPVIDKQKKHFVFFA
                     EDISTQITKFSDPPKTVGLAAAVGA"
     gene            685..1110
                     /locus_tag="ACF2SP_001940"
     CDS             685..1110
                     /locus_tag="ACF2SP_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359722.1"
                     /translation="MRRHFIIIVLGIFQFGCIPLAEHHNKISPATTHEEYHDIDFSNL
                     YEKNRSFPALETGIYAILDGILISSSQDTHLGKKQSKLLEEYVRQNRLFCQKGFDQEW
                     MNQLKQNLDRLEKTENNRIASAVNKKVQVLFAERQCNTW"
     gene            1385..2218
                     /locus_tag="ACF2SP_001941"
     CDS             1385..2218
                     /locus_tag="ACF2SP_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2541 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359723.1"
                     /translation="MLMEFEDWFLEQISNGKKCILIGENHYSYASEDCLDCILKLQRQ
                     GKINKKIVFFDENLAALDNLEPYTQESLSKLNTVEPSLIALIKNNVEVYGLEDNFSNP
                     FKNLKSIKKVTARLKDLGLQIGNRIFNDFEHCLYWACHLYGKSLVRVQRGNEGFCNQI
                     LHKMDEDTIVIARVGAKHIPTVRKDGVVIEDGMLQRLGKDMASACFITHFPKNKNYSE
                     QKTYQPESDMFGSYDSIECCMVQKKMIHQPDLTLWAEQDKQRKDANNYKDTKKHCCTI
                     M"
     gene            2333..2716
                     /gene="mscL"
                     /locus_tag="ACF2SP_001942"
     CDS             2333..2716
                     /gene="mscL"
                     /locus_tag="ACF2SP_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFD2359724.1"
                     /translation="MSLLKEFKEFAMRGNVIDLAVAVVMGVAFNKIVTALVDGIIMPC
                     VGLLLGGINIAGLSFTVGDAQIKWGSFLQNVIDFIIVAFAIFVLIKLINLLQRKKENE
                     PEPVTPEIQLLTEIRDLLARNSSKI"
     gene            2915..3322
                     /locus_tag="ACF2SP_001943"
     CDS             2915..3322
                     /locus_tag="ACF2SP_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2539 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359725.1"
                     /translation="MKLIINKKNRAESIALDVEATTTVNELLLILYTHRSKRYESLSF
                     NEYQKFISVYQCLLYGDQFINKLLPGTNLADYQLEDPCHLTWEEFTEKQCLLADAEAS
                     RILLWQANKKASKPEIEKDLMSVASSNAMNPDM"
     gene            3905..5321
                     /locus_tag="ACF2SP_001944"
                     /pseudo
     CDS             3905..5321
                     /locus_tag="ACF2SP_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948240.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2538 family Dot/Icm T4SS effector"
     gene            complement(5445..6464)
                     /locus_tag="ACF2SP_001945"
     CDS             complement(5445..6464)
                     /locus_tag="ACF2SP_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFD2359726.1"
                     /translation="MIVDFETHFITEACIDYLTQRQEVPKLVPELNGAYTMCFTPDVS
                     LFHTSALMEELLSLNEQRLAIMDQAGVTIQVLSLTTINGIDSCPGDENKSTALAREVN
                     DQLYSAIQKHPERFKGFASISPYDVKEGVKELERAISQLGFVGWLTHSDFGQDNYLDD
                     KIYWPLLEAAESLNIPIYLHPNVPIMREFGKYGFALGGSALGFEFDTALCLMRMILGG
                     VFDAFPKLKIMLGHLGETMPFLMERLDHLYRIPDLKPYRPSIQRIPSEVLRQNVYITT
                     SGRFFAPALRYVLEVMGEDRVLFASDYPMESLLDATRFIQDSDLSNQTKQKIFSINAK
                     NLNLI"
     gene            complement(6689..7645)
                     /locus_tag="ACF2SP_001946"
     CDS             complement(6689..7645)
                     /locus_tag="ACF2SP_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFD2359727.1"
                     /translation="MSTVRFNNQSFALTPDESLLQCFLRHGVEYPNSCQAGICQSCLI
                     KAKDGEINPDWQEGLPETLKSQGYFLACLAKPSTSLYVASPDNAECETQAQIMSLQRL
                     NHNVIQVKLKVEHPAPWLPGQYLSLINPEGTMRNYSIANIPADDGYIELHIKIYPQGK
                     MGQWLLQRAAINTFITIRGPFGQCYYHNPHNLTFDILLAGTGTGLAPLIGIIRCALTQ
                     KHEGTITLVHGGVTDEDIYYKEELEMLSLLFSNFRYDPCVLQSQGLYPEGSIEKRVLT
                     HLHSPNTTKVYVCGPKETTNKLKTRIFLAGVPSTNIYSDIFL"
     gene            complement(7642..8016)
                     /locus_tag="ACF2SP_001947"
     CDS             complement(7642..8016)
                     /locus_tag="ACF2SP_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HFD2359728.1"
                     /translation="MTESLFERLGGQNAVNTAVDIFYRKMLMDDRVNYFFDDVDMEQQ
                     ILKQKGFLTMVFGGPNQYTGKSMREGHQHLLARGLNDSHVDIVIEHLGETLKELGANE
                     EDIQKVAAIANSVRDDVLGRSS"
     gene            8096..8527
                     /locus_tag="ACF2SP_001948"
     CDS             8096..8527
                     /locus_tag="ACF2SP_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFD2359729.1"
                     /translation="MQLTQFTDYSLRALIYIALKKGSCTIKDITDAYSISNNHLIKII
                     HNLSKMGLIKTIRGKNGGILMAASPETINLGQLIMELEPNFDLVPCFNKEKANCCIAP
                     ACKLKSVLYEAQRAFLQILEQYTLADVLHNPSELSVLLRIS"
     gene            complement(8560..9456)
                     /gene="tehB"
                     /locus_tag="ACF2SP_001949"
     CDS             complement(8560..9456)
                     /gene="tehB"
                     /locus_tag="ACF2SP_001949"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFD2359730.1"
                     /translation="MPREYDNLICYKQLSIDDHGKLKFFLEKHSTKEGTWGKLSLNEG
                     EIDFIFQNGLGQELSRIRLDKNNRQLSIPPASWHKIIPISASFDAQLGFYCKPHRYFN
                     KKYGLTEVHRDLLYVYHTYLSHLGSLTTLDVGCGSGRNLLYLAQLGHRVTGIDINQTA
                     LENIQIIAQKENLSPLNILSQDLNQPQSLTPDHYDFVCSTVTLQFLNASCIPTLLIEL
                     QEATKKNGYHFLVFPVQSELYSLPSSFTFLPKQEELYHFYQDSGWSILEYKESVGHLH
                     KQDELGRPVQGLFALLLAQKVI"
     gene            complement(9634..10800)
                     /locus_tag="ACF2SP_001950"
     CDS             complement(9634..10800)
                     /locus_tag="ACF2SP_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFD2359731.1"
                     /translation="MNHYEKMSLLKDSATVAINSLAQQKIHAGIKIHNLSAGEPKLPP
                     HPSIINAVTQAMEQGHTLYPPIMGIPELRHMGCEWMYRSYDCSFQNEQCLIVNGGKLG
                     IYLLMQLLLQPKDEVIIPSPYWVSYPAMVSLFGGTPVPVETTEAEEWKLTPQALQKAC
                     TTKSKILILNNATNPTGALYTQSELIHLLQIAKKHNLLVISDEVYSELTYDGHHYVSC
                     GSLPQFRERVIIIQSCSKNFSMTGWRVGFIFAPEIFIKPLSALVSQSTSGVATLSQWA
                     AVAALQEINQVNIWVQQNMEKRRNCLLRTLHDYLGLTITPPISSLYVFLSLKDIGCKN
                     QNSEEFCKQALDKANVAMVPGIAFGKEGYVRLSFGGNEDDLKSGIYALAQWLHR"
     gene            complement(10800..11060)
                     /locus_tag="ACF2SP_001951"
     CDS             complement(10800..11060)
                     /locus_tag="ACF2SP_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFD2359732.1"
                     /translation="MGTIEELRKQIEQTDADIIEKLAQRQELAKQIGALKAKEGKKII
                     DRLREKKLFEYYDFLSRQYHLQQDFVNQLFKIIISNSKKVQK"
     gene            complement(11094..11678)
                     /locus_tag="ACF2SP_001952"
     CDS             complement(11094..11678)
                     /locus_tag="ACF2SP_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFD2359733.1"
                     /translation="MNNLVEKKESFRFGVSGDIGSFSEEAALLYARKKGLNAHLVHLL
                     DMEGVLNAIENETIELGVFPVVNLLGGLVKPAFQAMGKYSFTPIDEIWHDINQCLLVK
                     SSNQHHKITHIVSHPQGFAQCKKFLRNKFKNAKKIEWIDTAKAAKDLAEGILPPHAAV
                     IASARCAPLYDLKISARNIQDSHLNRTAFILVKK"
     gene            complement(11675..12718)
                     /locus_tag="ACF2SP_001953"
     CDS             complement(11675..12718)
                     /locus_tag="ACF2SP_001953"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFD2359734.1"
                     /translation="MSYSILKKLPPIEKIIRELPLSHPAYLQIAQDRQEIKAILEGRD
                     KRLLMIVGPCSAWPKKAVLEYAERLVQLNSKVKHALKLIMRVYIQKPRTTKGWTGPVN
                     QPDLFSTPDIEAGIKYTRDMMIKVIQMGLPIADEALFTHNAKGFLELLSWVAIGARSS
                     EDQEHRIFASALDCAVGLKNPTHGSLAVGVNSLVASQHPHVAVFDGYEVQTHGNHHAH
                     LVLRGSNQAPNYSVSHLEEVMYYMGMHQIVNPSVIIDVSHDNCLVNGKKNHQLQPEIA
                     FNILESIKNRPDLKQLVKGFMVESFIKEGNQKLDSINPDNIDLSGLSVTDPCLSWEKT
                     ESFLLELAEREAL"
BASE COUNT         4093 a         2405 c         2719 g         4117 t
ORIGIN      
        1 gaataagact tttattaccg atggattggc tacctgcgct gctgttgcta tctacggcag
       61 tgatagcggt tgttctgctg catttactca tatgtccagc gaagcttcgg acaaggatga
      121 taaacgaaaa gaaacgatat taaatgagat gatggaatat gttttaaaaa gcaattcatt
      181 atccgatgtt aaattaatta tttcgccttc caggattcaa gaaaaacatt tgatttcctt
      241 tattctggaa tgggtaaagc aaaaaaaaat tgcttgctcc atgctagaaa cagggggggg
      301 attcagcagt attcaatata gataaggaca gtcgtgtatt aatgctttca acttcactgc
      361 aattaaagca ggatgtatcc gataaaacat ggcatggtca tggtattatc attagccaga
      421 gcgagggtat tccagtgatt gataagcaga aaaaacactt tgtattcttt gcagaggata
      481 tttccactca aattaccaaa ttttcagacc ctcctaaaac agttggctta gcagcggcag
      541 taggcgctta gaaacaagca taagctgggt aaaatgaaat gaatttcgta ttatagtcaa
      601 aattgaaata tttcctcaat gctgggtaga tatgttaatt tcataatgga ttgtttttta
      661 tgctcaattt gtaggtggtg tatgatgaga agacatttta ttattatcgt gcttggtata
      721 tttcagttcg gctgtatccc tttggcagaa catcataata agatttcacc tgctacgacg
      781 catgaagagt accatgatat tgatttttcc aatttatatg aaaaaaatcg ctcttttcca
      841 gcattagaaa ccggtatcta tgctattttg gatggaatct taatttcctc aagccaggat
      901 acgcatcttg gtaaaaaaca atcgaaactt ctggaagaat atgtgcgtca aaatagatta
      961 ttctgtcaaa aaggttttga tcaggagtgg atgaatcaat tgaagcagaa tttggacagg
     1021 ttagaaaaaa ccgaaaacaa tcggattgca tctgctgtaa ataaaaaagt gcaagtgctg
     1081 tttgctgaac gacagtgcaa cacatggtaa ccagggctcg ccgttttatc aggcagcctg
     1141 gtccaaatca catgtgaact caatctttcg aaccattgat cacagtattt tttgtgcagg
     1201 atatcatgtc atgagaaggg tggttttggt tcaattttca actccatacc atgtagctag
     1261 ggataaaaac gggaaagaca agcaattaat actgtatttt tatcaatcat attgattatt
     1321 atttaattat ttagtataat aataaccttg attgggttat tgctctattg tcttttagtg
     1381 agatgtgcta atggaattcg aagattggtt tctggaacaa ataagcaatg gtaaaaaatg
     1441 catcttgata ggggaaaacc attattccta tgcaagcgag gactgcctcg attgtatttt
     1501 aaaattgcaa aggcaaggta agataaacaa gaaaatagta tttttcgatg aaaatttagc
     1561 tgcattagat aatttggaac cttacactca agaatcttta agcaaattaa atacagttga
     1621 accttcttta attgcattaa ttaaaaataa tgttgaagtt tatgggttag aagataattt
     1681 cagtaatcca tttaaaaacc ttaaatctat taaaaaagtg acggcacgtt taaaagattt
     1741 gggtttgcaa atagggaatc gaatatttaa tgactttgaa cactgtttgt actgggcttg
     1801 ccatctctat ggaaaatcat tggtcagagt acaacgcggt aacgaaggat tttgtaatca
     1861 aattttgcat aagatggatg aggatactat cgtcattgcc agagtagggg ccaaacatat
     1921 ccctacggta cgaaaagatg gagtagttat tgaagatggg atgctgcaac ggttaggcaa
     1981 agacatggct tcagcctgtt ttattacaca cttccctaaa aacaagaatt attcagaaca
     2041 aaaaacgtat cagcctgagt ccgatatgtt tggaagttat gattctatag aatgttgcat
     2101 ggttcaaaag aagatgattc atcaaccgga tttgactcta tgggcggaac aagataaaca
     2161 aagaaaagat gccaataact acaaagatac taaaaaacac tgttgtacaa taatgtaaat
     2221 ttaagttcct ttctacaagc taacagactc agaacagtat gtaattgaaa aattagaaaa
     2281 attttgcgaa tatacatttc atcgcataac ttaatcatga tagggatgtc tgatgagttt
     2341 actaaaggaa ttcaaagaat ttgccatgcg aggcaatgtg atagatttgg cagttgctgt
     2401 tgtaatgggt gttgctttta ataagattgt cactgctttg gttgatggaa ttatcatgcc
     2461 ttgtgttggt ttgttattgg gaggcatcaa tattgcaggg ttgagtttta ctgtaggaga
     2521 cgctcaaatc aagtggggaa gttttttaca aaacgtcatt gacttcatta tcgttgcttt
     2581 tgctattttt gtactcatta aattaatcaa tctgctgcag agaaaaaaag aaaatgaacc
     2641 agaaccagtc actccggaaa tccaattact tactgaaata cgtgatctgc ttgcaagaaa
     2701 tagtagtaaa atctgagcct ggcaggcaaa aataaatctc tatttatacc ctattcaaaa
     2761 tggtttgctg aacaacttat gttttatttc ggtgagattt atacagaatt ctggcgtttc
     2821 aatcggccac ttaaatctca caaagccata tattgattta ttgctattta aactatacta
     2881 gcagacaagg ctaattttta cttaatgatc gcaaatgaaa cttataatta ataaaaaaaa
     2941 tagggcggag tcgatagcgt tggatgttga agcaacaacg acagtgaatg agttgctttt
     3001 aattctttat actcatcgca gtaaaaggta tgagtctttg tcttttaatg agtatcaaaa
     3061 atttatttcc gtttaccaat gtttattgta tggagatcaa tttataaata aattacttcc
     3121 ggggaccaat ttagctgatt atcagcttga agatccatgt catttgacct gggaggagtt
     3181 tactgagaag cagtgccttt tagcggatgc agaggccagt agaatccttc tttggcaagc
     3241 taataagaaa gcaagtaaac ctgaaattga aaaagatctt atgtccgttg caagcagtaa
     3301 tgctatgaat cctgatatgt aacaagaata aaatgtcctg tattacatag tgagtgttcg
     3361 ttttgtaatt cattacttga gtcatgcaat gaaaaaaatt aaatacatcc aatcagttaa
     3421 ccagtgaaag gtcttgagtc ctctatggcc tcaatataag gtgttgccca tttaataaat
     3481 ccttaagctt ttattgttaa aattttgctc acataaaaga tcataaaaaa tgaacttttt
     3541 gctgcggcct atcttctcca caaggatatt tggaggactg gtggtaacac ttagctttaa
     3601 ttaagtaaac gggttcgtcg ctgaggcaaa gaaaatgagc gatttactct cggatttggt
     3661 gattgagagt agtttattaa ctttaattca gcgaggcgtt acttaacaaa tcgtcgtttc
     3721 ctttcttttt gtctctaaat tgaaatatcc atggcctctg tcagtcttta ttaggcagag
     3781 ggtttgttaa tttataagga attcataatg aaaaataatg attttaataa tcagttgttt
     3841 cattgagata gagcaagggg ttgtcattat gaaatcaatg aaaatcatcc cggtggacaa
     3901 ttaattgatt ccagtcctgg tgatgagaaa aattcatgaa ttagtaatga tgcaggctgc
     3961 agccggggtt ttttatgaaa acaaaatatg aaatgagtca agataaaact gagtttttgg
     4021 caaaagaact agatagtccc tatccaggat atcaagttag cgttctagat ctcgaaaaaa
     4081 tagttaagca ttatcaggaa aaatacggta ttcgcttgat tattaatggc actacaccca
     4141 aatatcaggc tctgattaaa gagcgccagg ttgattttga acaacaaaaa cagcaattcc
     4201 tagagttcaa atacgccaaa tttttacaaa tattttttca accacctaat ctgaatggcg
     4261 caaactctcc tttttctatc aataaacata tgggcgcatt tataggtttt tacgaagaaa
     4321 tatacaataa agttcttcca tttctggatg caaaaggaaa aaccatttct ggattatccc
     4381 tggaagagtt aagacaactc aatgaagcct gccaggagct gtcatgcaaa ggaatacttg
     4441 atgcaaaaat taatgaattt atagagcgaa attttgatta tatggggtta actgccaggg
     4501 aaagcgcaag tgaaataaaa gacatctgtg atgaactgca agaaggagag gttttgggtt
     4561 atttctttac tggtcaacgt accagtggga gatgtcattt cgatctctat atctgtttac
     4621 ctggtaaggc cataagaccg atattttata acacagcgct tgtcagatat catgatcttg
     4681 gaggggtgtt tcatttgaat tttccttttg tcgaaggcaa tttttttaca cctgatttgt
     4741 taaaattata tcctgcattg gatctgatac agctaatccc tcaagctgac aggacttcat
     4801 gtggtacttt gacaatgatg tatgccaagg agttattaaa agatgatgcg agagggctga
     4861 aagaatttac gttgagtttt acttattata atgagaaagg agaaaaggaa tatttttttc
     4921 tcccatcacc acaagtatta cgatattctc agataagttt atataatgaa gcgctgaaag
     4981 ccattgtatc tcacgcaaat gatggacaag ctggtttggt caggaaaggc gctaaaaaat
     5041 atatgtttca aactatcgaa acaattctca tccaatcttt taagattgca cttgagaaag
     5101 aagatgccga tgttctggag gaaaatcaaa aaatttggga tatattaccc agcttccagg
     5161 aaaaatggca ggaggcctat aaggaaatgg ttgccaaacg cgatgtgatg caccaagggg
     5221 taaataaata tttgctttat tccacccatc ggatgagcca tattgcaagt gatcaatcca
     5281 taaacaatga agcggatgct gataggttaa ttcttcgtta aaaaatattg cagaatgaaa
     5341 ctgttcgtat tttatttata gaaagcatga cattaattga gcctggctca attaatgtct
     5401 gcaagaaaat cagctcctta ctgataagat gtaagagata tgattcatat taagttcaaa
     5461 ttcttggcat ttatagagaa aattttttgc ttcgtttggt ttgataaatc ggaatcttga
     5521 ataaatcgag tggcgtcaag taaggattcc attgggtaat cggaggcaaa caataccctg
     5581 tcctctccca tgacctctag aacataacgt aaggcgggag cgaagaaacg tccactggtg
     5641 gttatgtaaa cattttgacg taatacctct gagggaatac gctgaataga aggtcgataa
     5701 ggtttcaagt ctggtatacg gtataaatga tcgagacgct ccattaaaaa aggcatggtt
     5761 tcgcccaagt gccccagcat aatttttaat ttgggaaacg catcgaatac acctccaaga
     5821 atcattcgca tcagacataa agccgtgtca aattcaaacc ctaaagcact tccccccaag
     5881 gcaaaaccat atttaccgaa ttcgcgcatg atgggtacat tcggatgcag gtagatagga
     5941 atgttcaaac tttccgcggc ttccagcaat ggccaataga ttttatcgtc caggtaatta
     6001 tcctggccaa aatcggagtg tgtaagccaa cctacaaagc ctaattgaga tatcgctctt
     6061 tccaattctt ttacaccctc tttgacgtca taaggcgata tagaagcaaa acctttaaat
     6121 cgttcaggat gtttttgaat ggcagagtaa agctggtcat tgacttctct tgctaaagcg
     6181 gtgcttttgt tttcatctcc agggcaggag tcaatcccat tgatagtagt caaacttaat
     6241 acctggattg tcacccctgc ttggtccata attgccagtc gttgctcgtt taaacttaat
     6301 aattcttcca ttaatgcact tgtgtgaaat aaggaaacat caggtgtaaa acacattgta
     6361 taagctccat tgagctctgg aaccaatttg ggaacttcct ggcgttgggt cagataatca
     6421 atacaagctt cagtgatgaa gtgcgtttca aaatcgacga tcatgaacgt aatcctttta
     6481 gacgtgagaa aaacgattga aaattatatg tttaagtata gccggaagat tagataccgc
     6541 aacatggcta taaaaattct gatggcaaat tgccggaaaa cgtcttaaag ataagaaatg
     6601 ctaccagcgg aaagaagaga tcattttgat gagcgagaga cataatctgg tttgcaccag
     6661 attataaatc aacagggcta tttaaatatt ataaaaaaat atcactgtaa atatttgttg
     6721 aaggaacgcc cgcaagaaaa attcgtgttt tcaatttgtt ggttgtttcc tttggaccac
     6781 agacatacac ttttgtagtg ttgggagaat gcaggtgagt tagcacccgt ttttcaattg
     6841 agccttctgg ataaagccct tgactttgca gcacacaggg atcatagcgg aagttgctga
     6901 acaataaaga caacatttcc agttcttcct tgtaatagat atcctcatca gtaactccgc
     6961 catgcaccag ggtaattgtt ccttcgtgct tttgtgttaa tgcgcatctg atgataccta
     7021 tcagcggagc gaggccagtc cctgtccctg ccagcaatat atcaaaagtc agattatgtg
     7081 gattatgata ataacattga ccaaaagggc ctcggatggt gatgaatgta ttaatggctg
     7141 cgcgttgcaa aagccattgt cccatcttgc cttgtgggta aatttttatg tgtagttcaa
     7201 tgtatccatc atcagcaggg atattggcta tggaataatt gcgcattgtt ccttcaggat
     7261 taatgagcga caggtattgt ccgggtagcc atggggcagg atgttccacc ttcagtttga
     7321 cttgaataac attgtgattt aagcgttgca ggctcattat ttgagcttgt gtctcgcatt
     7381 cggcattgtc aggagacgcg acataaagcg aagtgcttgg tttagcaaga caagccagga
     7441 aatacccttg agatttgagt gtttcgggta atccttcttg ccagtctgga ttaatctccc
     7501 cgtctttggc tttaatcagg catgattggc aaatgcctgc ctggcaggag ttagggtact
     7561 ctaccccgtg gcgcaaaaaa cattgcaaga gactttcatc cggagttaat gcaaaagatt
     7621 gattgttaaa tcgtacggta ctcatgatga gcgccccaat acatcgtcgc gaacactatt
     7681 ggcgattgca gctaccttct gaatgtcctc ttcattggcg cccaattcct taagggtttc
     7741 ccctaaatgc tcgattacaa tatccacatg tgagtcattt aagcctctgg caagtagatg
     7801 ctgatgtcct tcccgcatac tttttccagt gtattgattt ggcccgccaa acaccatggt
     7861 taaaaatcct ttttgcttaa ggatttgttg ctccatatcc acgtcatcaa aaaaataatt
     7921 cactctgtca tccatgagca ttttgcggta gaaaatatcc acagccgtgt tgacggcatt
     7981 ttggccacct aatcgctcaa acagtgattc agtcatgata tactccttaa agatgtattt
     8041 gaaatgcatc ttataaaaag aaataataga cgtcaacaaa ccagaaggat tttttatgca
     8101 attgactcaa tttactgatt attccctgcg tgctttgatc tacattgcct taaagaaagg
     8161 ttcatgtacc attaaggaca ttacagatgc ctattccata tcaaacaacc atctcatcaa
     8221 aatcattcat aatttatcga agatgggttt aattaagaca atacggggga aaaacggcgg
     8281 tattttgatg gcggcctcac ctgaaaccat taatttaggc caattgatta tggagcttga
     8341 gcccaatttc gatttggttc cctgttttaa caaagaaaag gcaaactgct gtattgcccc
     8401 agcatgtaaa ctcaagagcg ttttgtatga agcgcaacgt gctttcctgc agattttaga
     8461 gcagtatact ctggctgatg ttttacataa tccaagtgaa ttgtctgttt tattgaggat
     8521 cagttagcaa tccagagtaa tattttatga aagtgatctt tatataactt tctgggcaag
     8581 caacaaagca aataatcctt gtacgggtct tcccaattca tcctgtttat gaagatgtcc
     8641 tactgattct ttgtattcga ggatagacca gccgctgtcc tggtaaaaat gatacagctc
     8701 ttcttgtttt ggtaaaaacg tgaatgagct tgggagtgaa taaagttcag attggacggg
     8761 aaaaaccaga aaatgatagc catttttctt cgttgcctcc tgcaactcaa tcaataaagt
     8821 ggggatacat gaggcattta aaaattggag cgtcacggta gagcagacaa aatcgtaatg
     8881 atctggtgta agggattgag gttgatttaa atcctgcgac aaaatattaa gcggactcag
     8941 gttttctttt tgagcgatta tttgaatatt ttccagtgcc gtttgattga tatcaattcc
     9001 agtcactcta tggccaagtt gggctaaata caacagattt ctaccagaac cacaaccgac
     9061 atccagagtg gttagtgagc ctaaatgcga caagtaggta tgatacacat acaataagtc
     9121 acggtgcacc tcagttaagc cgtatttttt gttaaagtag cgatgaggtt tgcaataaaa
     9181 accaagctga gcatcgaacg atgcgctgat cggaatgatt ttatgccagg aggctggagg
     9241 aatagataat tgcctattat ttttatcaag acgaatacgc gacagttcct ggcccaagcc
     9301 attctggaaa ataaaatcaa tttcaccttc gtttagggaa agtttgcccc atgttccttc
     9361 tttagtgctg tgcttttcaa gaaaaaattt aagttttcca tggtcatcaa tggatagttg
     9421 tttgtagcag attaaattat cgtattctct tggcatggtt ttttatcctg aggtatcgat
     9481 aaataacata atagtttttt agcctattgt cgaatgcatc aattttatag agccgttaaa
     9541 tggagcaggt tattaactgc aaaactggag taaaataatc agattaggca agaggtctag
     9601 caatgcttct tgcaaagtaa tgaattactt taatcagcga tgcagccatt gcgccagagc
     9661 ataaatacct gattttaaat cgtcttcatt gcctccaaaa gacaagcgga cgtaaccctc
     9721 tttaccaaag gcgattcccg gtaccatcgc aacattggcc ttatccaaag cttgtttaca
     9781 aaactcctcg gaattttgat ttttgcagcc aatatccttt agggaaagaa aaacataaag
     9841 tgaggaaatt ggaggtgtta tggtcaatcc cagatagtca tgcaaggtgc ggaggaggca
     9901 atttcttctt ttttccatgt tttgttgaac ccagatattg acttgattta tttcttgcag
     9961 cgcagccaca gcggcccatt ggctaagggt ggccactcca ctagtgcttt gactgaccag
    10021 ggcgcttaaa ggtttgatga atatttccgg ggcaaaaata aaacccactc gccacccggt
    10081 catggaaaag ttttttgaac agctttgaat gatgatgaca cgctcccgaa actggggtaa
    10141 agaaccacag gatacgtaat gatgtccgtc ataggtcagt tcgctgtaaa cttcatcaga
    10201 gatcactaaa agattatgct tcttggcaat ttgcaataag tgaatcagct cggactgggt
    10261 atacagagct ccggtgggat tggtggcatt attcagaatg agtattttac tttttgtggt
    10321 acacgctttt tgcagtgcct gtggcgttag tttccattct tcggcttctg tcgtttcaac
    10381 tggcacgggg gtgccgccaa acaggctaac catggctgga taggaaaccc agtagggaga
    10441 aggaataatg acttcgtcct taggctgcag taataattgc ataagtaaat aaatgccaag
    10501 ttttccgcca ttcacaatca ggcattgctc attttggaat gagcaatcat aggatctata
    10561 catccattca cagcccatat gacgtagttc aggaatcccc attattggag gataaagggt
    10621 atgtccttgt tccatagctt gtgtgacggc atttattatg gagggatggg gtggcagttt
    10681 gggttctcca gcactgagat tatgaatttt gattcccgcg tgtatttttt gttgggctag
    10741 agaattgatg gcaactgttg cggaatcttt tagcaagctc attttttcat aatggttcat
    10801 tatttttgga ccttttttga gtttgagata attatcttga ataattgatt cacgaaatcc
    10861 tgctgcaagt gatattgtct ggataaaaag tcataatatt caaacaattt tttttcgcgc
    10921 aggcgatcta ttattttttt tccttcttta gcctttaaag caccgatttg cttggccaac
    10981 tcttggcgtt gagccaactt ttcaatgatg tctgcatcag tttgctcaat ttgttttctt
    11041 agttcttcaa tcgtacccat gatgaactcc ttagcggata gggaccttat cacttatttt
    11101 ttaactaata taaaagccgt tctgttcaga tggctgtcct gaatatttct cgcactgatt
    11161 tttaaatcat aaagtggtgc acaacgtgcc gacgcaatga ctgcggcatg gggtggcaat
    11221 attccttctg ccaaatcctt tgctgctttt gctgtatcga tccattctat ttttttcgca
    11281 ttcttaaatt tgtttcttag gaattttttg cattgggcaa agccttgagg atgtgacaca
    11341 atatgtgtga ttttatgatg ttgattactc gatttaacca gcaggcattg gtttatgtca
    11401 tgccaaatct catcgatggg agtaaatgaa tatttgccca tagcctgaaa tgctggcttt
    11461 accaatcctc ctaaaaggtt aacaacgggg aatacgccca gctctatcgt ttcattctca
    11521 atggcattta aaacgccttc catatccagc aaatgaacga gatgggcatt aaggcctttt
    11581 ttcctggcat aaagcaaagc tgcttcctca gaaaatgatc caatatcccc tgaaacacca
    11641 aagcgaaagg attctttttt ttcaacgaga ttattcataa tgcttccctc tccgccagtt
    11701 ccaacaggaa cgattctgtc ttctcccaac tgaggcaagg atcggtaacg gataacccgc
    11761 ttaaatcaat gttatcggga ttgatagaat ctaatttttg attaccttct ttgatgaaac
    11821 tttctaccat gaatcctttg accagttgtt ttaaatcagg tctatttttg atgctttcca
    11881 gaatattaaa tgcaatctct ggttgtagct gatgattttt tttcccattc accagacagt
    11941 tgtcatggct aacatcaata atgacagaag ggttcactat ctgatgcatg cccatgtagt
    12001 acatgacttc ctccagatga gaaacggagt aattgggagc ttggtttgaa ccacgcagaa
    12061 cgagatgagc atgatgatta ccatgggttt gcacttcata gccatcgaag acagcaacat
    12121 gaggatgctg tgatgcaacc aggctattta ctccgacagc gagtgaacca tgtgtagggt
    12181 tttttaatcc tacggcacag tcaagggcgg aggcaaaaat tcgatgctct tgatcctctg
    12241 aactacgggc gcctattgca acccaggaca gaagttcaag aaatcctttg gcattatggg
    12301 tgaaaagagc ctcatcagcg atagggagcc ccatttgaat gacttttatc atcatgtccc
    12361 gggtatattt tatacctgcc tcaatatcag gagttgaaaa taaatcgggt tgattcaccg
    12421 gtccagtcca gcctttggtg gttcttggct tttgaatgta aacgcgcatg attaatttta
    12481 aagcgtgttt tactttgctg ttgagttgta ccaaacgctc agcgtattcc aataccgctt
    12541 ttttaggcca tgctgagcaa ggaccaacta tcatcaaaag acgtttatct cgcccttcca
    12601 aaatagcttt gatctcttgc cggtcttgag caatttgtag ataggcgggg tgggatagtg
    12661 gcaattcccg gatgattttt tctattggtg gcagcttttt taagatggaa taactcatcg
    12721 catcctcctc aagccagaaa gcgctttaga tggatgatga atttgacatt gatggttcat
    12781 cgttcacact cctcttttgt acccaacccc cttattagac tggggatctt gggtatcaaa
    12841 aacccccaga ctcagctggg ggttggagtg ttagtattgc ctacctacca cccagctcag
    12901 ggctgaaaaa gctaaaataa aaaaagcaag caaaagcctg tacaagactt gcttttttat
    12961 ttaactgatt tggtgtagta gtgtagaaca tgaataatca caaaaaattt aatttgtata
    13021 ttatgtatac aggactatgg taaacctgtc aacacctgtt agcgacagtt aattttatag
    13081 ccaattttcc actgggaatt aacccaatga tttacatata aaaatcactt tggcattaat
    13141 cgcactcact gattggaaaa tgagatcaaa gtattgcgtg aatcattttg atcgtataca
    13201 ttgcatttta tatcaaataa aaactaaggg attaatgttg tgtcaatatg ttgggattta
    13261 atcataaatc ataatacccc gctgcaaacc aattttactg gtcaccgggt tcaaatgaat
    13321 gactccgaat ccac
//
LOCUS       DAXLDT010000085        13147 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXLDT010000085 DAXLDT010000000
VERSION     DAXLDT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.085"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..561)
                     /locus_tag="ACF2SP_001954"
     CDS             complement(<1..561)
                     /locus_tag="ACF2SP_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HFD2359735.1"
                     /translation="MLTLLSPAKKLLSISKHYSKETSNPLLLDKALQLVKIMKLKSVE
                     QIADLMDLSRQLAELNYERYQNFDLKNNPMNHSYPALFLFQGDVYQGLNANSWKDEEI
                     EYAQSHLGILSGLYGFLRPLDRIQPYRLEMGVNLENPAGKNLYAFWSKIVTNILNQIL
                     AEQSNPVLINLASTEYFKVVDEKKLSY"
     gene            complement(574..1134)
                     /locus_tag="ACF2SP_001955"
     CDS             complement(574..1134)
                     /locus_tag="ACF2SP_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFD2359736.1"
                     /translation="MNKLPLFVFLMMVICLSSAQIYKWTDSQGVVHFSDKPHQGAEKI
                     KIPEAQTYTPPANETGGSSEQEQKSVKQEDQEKYTKLEIIQPQNEATIRNNQGYVVVA
                     VQIEPDLSPGDNLQLIFDGAPLGEPQPNLIFQLNGIYRGSHTIAVQVLNDKGDVLKVS
                     DSITIFMHNPRVGMGKGRKKNINSNK"
     gene            complement(1147..2556)
                     /gene="glnA"
                     /locus_tag="ACF2SP_001956"
     CDS             complement(1147..2556)
                     /gene="glnA"
                     /locus_tag="ACF2SP_001956"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFD2359737.1"
                     /translation="MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFL
                     ENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYD
                     RCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
                     SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATAN
                     QCEVATRFNTLTKKADELQILKYVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLY
                     KDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLA
                     YSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQP
                     MDKDLYDLPPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEE
                     ITKIRSLTHPYEFELYYSL"
     gene            2653..3852
                     /gene="lspF"
                     /locus_tag="ACF2SP_001957"
                     /gene_synonym="gspF"
     CDS             2653..3852
                     /gene="lspF"
                     /locus_tag="ACF2SP_001957"
                     /gene_synonym="gspF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspF family T2SS innner membrane protein variant
                     LspF"
                     /protein_id="HFD2359738.1"
                     /translation="MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQT
                     LTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGV
                     RSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
                     VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSY
                     GLYSLVTIIVALIGFKKSLSNIKIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILF
                     AAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALKETKFISPMAIHLIA
                     SGEKSGQLSEMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIF
                     SMEQLVA"
     gene            3956..4378
                     /gene="lspG"
                     /locus_tag="ACF2SP_001958"
                     /gene_synonym="gspG"
     CDS             3956..4378
                     /gene="lspG"
                     /locus_tag="ACF2SP_001958"
                     /gene_synonym="gspG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspG family T2SS major pseudopilin variant LspG"
                     /protein_id="HFD2359739.1"
                     /translation="MNRQKGFSLIEIMVVVVILGILASIVVPKIMGRPDEARKVKAKQ
                     DVLAIQNALDLYKLDNGSYPTTDQGLMALVEKPTSNPVPTNWKQYLKSLPKDPWGREY
                     LYLNPGQHTDVDVFTYGAAGQPGGTGINAEIGNWDDHS"
     gene            4365..4850
                     /gene="lspH"
                     /locus_tag="ACF2SP_001959"
                     /gene_synonym="gspH"
     CDS             4365..4850
                     /gene="lspH"
                     /locus_tag="ACF2SP_001959"
                     /gene_synonym="gspH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH family T2SS minor pseudopilin variant LspH"
                     /protein_id="HFD2359740.1"
                     /translation="MTTHKGFTLIEILIVIVIIGITLGFALIAFGDFGESRRIQFSAE
                     QLANTLSLAQQQAILESSTLGLKIDNTSYQILKFQDQAGWNPISNKGIFKLYYFPKNV
                     VISLKTNQKHTRGNPAIIIAPSGDMTPFTIEFGTDKEKWIAKLTGSHNGDLDYTMAKS
                     Q"
     gene            4847..5224
                     /gene="lspI"
                     /locus_tag="ACF2SP_001960"
                     /gene_synonym="gspI"
     CDS             4847..5224
                     /gene="lspI"
                     /locus_tag="ACF2SP_001960"
                     /gene_synonym="gspI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspI family T2SS minor pseudopilin variant LspI"
                     /protein_id="HFD2359741.1"
                     /translation="MMPKTKLVSGFTLIEVLLALTVIAIALTALLKATAQNIDNTHRI
                     KEKTISHWVAMQGVAMIQLNLLRTSQSQESTQATTMLGQKWYWRAKISQTPIKRMQQI
                     TISVSSKQAGPFREELIAFRYLP"
     gene            5221..5838
                     /gene="lspJ"
                     /locus_tag="ACF2SP_001961"
                     /gene_synonym="gspJ"
     CDS             5221..5838
                     /gene="lspJ"
                     /locus_tag="ACF2SP_001961"
                     /gene_synonym="gspJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspJ family T2SS minor pseudopilin variant LspJ"
                     /protein_id="HFD2359742.1"
                     /translation="MIKNKGFTLIEILIALTVFAILATITSSTLYYAFNTRTRVNAQA
                     ERLNALQLAISIIQQDTSQTVERAIRGNEMRLFPIFVGQPQYLELTRDGVVNPNSVEK
                     RSTLERVALICQDSKLLRRTWNTLDPIDRNIYKDKVLLDNLSECHFNYLNQNLQLLSE
                     WREQAVNQNQQREPLPKAIQMNLTLKDWGDMNMLFIIPGALYVYE"
     gene            5825..6793
                     /gene="lspK"
                     /locus_tag="ACF2SP_001962"
                     /gene_synonym="gspK"
     CDS             5825..6793
                     /gene="lspK"
                     /locus_tag="ACF2SP_001962"
                     /gene_synonym="gspK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspK family T2SS minor pseudopilin variant LspK"
                     /protein_id="HFD2359743.1"
                     /translation="MFMSSPLPKSIASSKMQGSALLTALFIMTLVAIIATAMSTRVQQ
                     DIYRTRLVIAQDKLYLASQAVTFWALNELLDKNNRFTKTNQLGMVAQYPKNMESIYNQ
                     VQLSGGIFDLQARFNLNNLIEKKSIPTLMHLISHAYSKAAGQERANIALGVKHWLLAY
                     DLGRGEDIYTSYYLSQKPPYYPSHQLIKSKSEFRLIKDVSAPAYIALEPFITALPEST
                     SININTAPKQILMSLGDGLSDAQANELITARGENGITDLKEINELLKKFNIPSDQITI
                     ESQYYLSVAYAKNDEFSLVVYSLLKRSRDRRGKLLTSVIRESINNF"
     gene            7108..9486
                     /locus_tag="ACF2SP_001963"
     CDS             7108..9486
                     /locus_tag="ACF2SP_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2359744.1"
                     /translation="MINIIKNRNKKNHTASTELKSEIEKEQVKLLFDQVGMAISGEAL
                     AVTILPIALWSVTNHELLIIWMIFTYVFSDLYKSMLLFYYNKQPTLFSTEKWLFLFNL
                     GVVSSGLAWGFASSIMIPATSTLHQIFVVFLITGVTAAANSLYSPVRLSYFLFLVTAF
                     VPFTIWLFSKGQVYLLLGFCGIIYMGIMLFISYYSNRILINTLKMRFKISNLNSTKDI
                     LEKKVVDRTKELEKILALTKSTLESTADGILVVDLKGNIEFCNQQFLKMWGISPGFID
                     SHNDTETIQYVLNQLKNPEEFLKQIKELYNNPKQESFDELHFKDGKIFERYSQPHWLD
                     NKIVGRVWSFRDVTLSTRLAYQANHDSLTGLPNRTALYDRLDHAINYCKRLNTSLSVL
                     FLDIDNFKLINDSLGHDAGDILLYEFSRRLKGCIRHSDTVARFGGDEFVLLLMTTEPK
                     DVILVAQNILKAVAQPIKLPTQEVIVTTSIGISLFPKDGIDASTLLKNSDTAMYLAKK
                     EGRNNFQFYNNSINQQSLKRLEIQTQIHNAIKNNEFFILYQPIYNLRSGELVGAEALI
                     RWGHPAIGLLSPDEFIPIAEETGLINQVGEWVLRNACIQNKTWQLMGLPHISISINVS
                     WRQLKNSNFIEIAESVLKESRLDPQYLEIEFTESTMIQKSTPIDSTLIKISQLGIKMA
                     IDDFGTGYSNLSYLKTFPFNKLKIDRSFIQNCTTNPNDASIVEAIIAMAHSLELKVLA
                     EGVDTIDKAFFMHQHGCDEVQGFLYGLPVKADKFVKLMQKTKIRHSKFINKT"
     gene            complement(9625..10755)
                     /locus_tag="ACF2SP_001964"
     CDS             complement(9625..10755)
                     /locus_tag="ACF2SP_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFD2359745.1"
                     /translation="MQGFVLALLLAISSSVFADELVGFAAFENGDYTTAYPHLMQAAK
                     EGNEEAMYLLGRMYQYGYGVTTNYEEARNWYQKAADKNNALAQLSLGFMYDTGKGVSQ
                     DFAEAFKWYMKAAEQGNPIAQRNIGLMYATGDGVAASDDKAFTWFKKAAEQGYSKAQV
                     NLGYQYMMGKGTPKDVKKAFEWYQKAAEQGDEKGEYSLGLLYTGQEGGISADDKAAFY
                     WFSQAANHGHVNAQTYLAYYYLKGYGVDADPVKAAYWYQSAAEKGQPEAQAQLGQLLL
                     TGTGVDKDYQQAAYWFGKSAHQGNPVGQAKLGYMYLAGLGVNKSLVKAYAWLKIAAEN
                     KNEEAAKQLKSLEAKLTEPEKLEAEKMIKDLGPLESKENYED"
     gene            10958..13147
                     /locus_tag="ACF2SP_001965"
                     /pseudo
     CDS             10958..13147
                     /locus_tag="ACF2SP_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947085.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein SidG"
BASE COUNT         4314 a         2522 c         2345 g         3966 t
ORIGIN      
        1 ataacttaat tttttctcat ccacgacttt aaaatactca gtcgaagcca ggttaataag
       61 cacaggatta ctctgctctg ccagtatttg gtttaaaata ttagtaacaa ttttgctcca
      121 aaatgcatag agatttttac ctgccggatt ttccaaatta actcccatct ccaatctgta
      181 aggttgaatc ctatctaaag gccttaaaaa cccatatagc cctgacaaaa ttcctaaatg
      241 ggattgagcg tattcgatct cttcatcttt ccatgaatta gcattcaatc cttggtacac
      301 atctccctga aataaaaaca aagcgggata agagtgattc attgggttgt ttttaagatc
      361 aaaattttga tatctctcat agtttaattc agcgagttgt cttgataaat ccattaaatc
      421 agcaatttgt tccacagatt ttaacttcat tattttaacc aattgtagcg ctttatcgag
      481 caataaagga ttactggttt ctttagagta atgttttgat atggataata gtttcttagc
      541 tggcgataac aaagttaaca tacataacct gctctattta ttagaattga tatttttctt
      601 tctgcccttg cccataccga cgcgaggatt gtgcatgaaa attgtaatac tgtcgctgac
      661 tttaagcaca tcccctttat cattaagaac ctgaacagca attgtatgag atcctcgata
      721 gataccattc aattgaaaaa tcaaattggg ctgtggctct cctaaaggcg ccccatcaaa
      781 aataagctgc aaattatccc ctggagacaa atcaggttct atttggactg caacaacaac
      841 ataaccttga ttattacgaa ttgttgcttc attttgcggt tgaataattt ctaatttggt
      901 atatttttcc tgatcttcct gtttgacact cttttgttct tgctcagaag aaccacctgt
      961 ttcatttgca ggtggtgtat aggtttgagc ttcaggaatt tttattttct cagcaccctg
     1021 atgaggtttg tcgctaaaat gaacaacacc ctgactatcc gtccatttgt aaatttgcgc
     1081 gctggaaagg caaattacca tcatcaaaaa gacgaatagt ggcagtttat tcatgaatat
     1141 agacccttag agactataat ataactcaaa ttcataagga tgagtcaagc tcctgatttt
     1201 agtaatttcc tcttctttca tttgaatata attattaata aagtctctgc tgaacacatc
     1261 tccctgtaac aaaaattcat gatctgcccg caaatggtcc atagccatct ctaatgaaga
     1321 acaaacagtg ggtacatcaa caagctcttc aggcggtaaa tcataaagat ctttatccat
     1381 tggctgccca gggtagattt ttttctgaat gccatctatg cctgccatca tcattgctga
     1441 gaaagcaaga taaggattag ctgttggatc agggaaacgt acttctatac gtcgtgcctt
     1501 tggattgcta acgtgtggga tacggatagc ggcggagcga tttcttgctg agtaagccag
     1561 taatacaggc gcttcaaacc cgggaactaa tctcttatag ctattggttg acggattggt
     1621 aaaagcgttt aaagctctgg catgcttgat tattccacct atgtaatata aagctgtttc
     1681 agaaagcccc gaatactgat caccggaaaa caggttatac ccatctttat aaagtgattg
     1741 atgacagtgc atcccgcttc cattatctcc taccaaaggt tttggcataa aagtagcggt
     1801 tttcccatag ttatgggcaa cattatgtat cacatatttc agaatttgca gttcgtctgc
     1861 ctttttggtg agtgtattaa aacgggtagc tacttcacac tggttagctg tagctacttc
     1921 atgatgatgt gcttccacaa ccattcctaa agattccaat gtgatacaaa ttgctgaacg
     1981 aatatcatga gatgaatcga ctggaggaac agggaaataa ccgcctttca acggaggtct
     2041 atggccaata tttccgcctt ccatttcctt accggagttc caatgtgcct cgtctgaatc
     2101 tattttataa aatgaaccgc tgatagtagt ttcccattgc acattgtcga agagaaaaaa
     2161 ctcaggctca gggccaaaat ttacaccatc tgcaatccct gtagattgta aataattttc
     2221 tgcgcgttgt gcgattgaac gtgggcatct gtcataaccc atcatagtct gtggatcaac
     2281 tacattacat cgtataaata aagtgttgtc ctgaaaaaat ggatccggct taattgaagt
     2341 taaatctggc actaaagcca aatctgattg atgaattttc tgccaacctt taaaagatga
     2401 gccatcgatc atttttccat tctcaagaaa atcatcatca actgctgaaa caggtatggt
     2461 aatgtgttgc tcttttccac ggatatctgt aaatcttaaa tccacaaatt tggcgtcatg
     2521 ttctttaatt gcatcaagca ctacattttt tgtcatcttt tgtctccagg atagtccgcg
     2581 tatagtttac cttaagtgac ttattaatcc caatagatta cactctatag ttttaatttt
     2641 aacaaaaata atatgggcgc ctaccaatat caagcgctaa aagtgaatgg cagcattagc
     2701 aaaggtgtaa tagaagctga ttctgaacgt catgcacgtc aattattgcg agaacaagga
     2761 ctaatcccta ctcaagtcca aacattaacc cagcaacgtg ctgccagcag caaaagtaaa
     2821 gtttctgcgg ctgatttggc attactcacg agacagctgg caacacttct ggctgccggt
     2881 attcctgttg aagaatcatt gcgcggggtc agcgaacaaa cagaaaaaga caaagtcaga
     2941 gagcttatta tcggagttcg ctctaaagta ttggaagggt atggtcttgc tcaggcaatg
     3001 gctcaatatc ccaatgcctt tccagaacta taccgggcaa cagttggctc aggcgagcag
     3061 acagggcgtc ttgatgtcgt tttagaaaaa ttagctgatt atactgaaaa acaacaacaa
     3121 actcgacaga aagtgcagca agccttgatt tacccattgc tcatgatcat tgtttctact
     3181 gccattatca gttttttact tacttttgtc gtccctaaaa ttattgatgt gtttaccgaa
     3241 agtggccaaa cactaccacc aatgacgcaa ttgcttatta acttgagcca atttattaaa
     3301 tcttatggtt tgtatagtct agtgacaata attgtggctc taattggatt taaaaagagc
     3361 ctcagtaata taaaaattaa aacagcatgg catcaattca tgcttaaact acctatcgtg
     3421 tcttatttgg ttaaaacaat taatgtcgcg cgatatattc acacgtttgg aattctattt
     3481 gcagcgggag tcagtgtact tgaaaccatg cgtgtttctt caagtttggt aaccaatatc
     3541 gtgatgagac aggcttttga tttagctact ctacgagtcc gtgaaggaag tggaatcagt
     3601 gaggccctca aagaaacaaa attcattagc cccatggcta tccatctgat agccagtggt
     3661 gagaaaagcg gtcaactttc agaaatgatg gaacgctcag cgtctcattt agataatgaa
     3721 gtaaaccgac tgattgaaac atccttaact ttattagaac ctatggttat tctattaatg
     3781 ggtgctgttg ttttatttat tgtactagcg accttattac ctattttttc aatggaacaa
     3841 ttagttgcct gattaagcgg agaaagggct atccgcgtcc ttttaaaatt taagtctatt
     3901 cataggcatc aatatttgtg ttataattta acatttagtt tcaagaggta cacaaatgaa
     3961 tcgacaaaaa ggcttttctt tgatcgaaat tatggttgtt gtcgtgattt taggcatatt
     4021 ggcatctata gttgtcccca agatcatggg acgtccggat gaagcccgta aagtcaaagc
     4081 caaacaagat gttttagcca ttcaaaatgc actcgattta tacaaacttg ataatggttc
     4141 ataccctaca actgatcaag gtttaatggc tctggtagaa aaacctacaa gcaatcctgt
     4201 tccaacgaat tggaaacaat atctgaaatc attgccaaaa gatccatggg gcagagaata
     4261 tctttattta aacccaggac aacatactga cgttgatgtt tttacttatg gggcagcagg
     4321 acagcctggc ggaacaggga ttaatgcgga gattggtaac tgggatgacc actcataagg
     4381 gttttaccct gatagaaatt cttattgtca tagtgattat aggtataacc ttggggtttg
     4441 ccttaatcgc ttttggtgac ttcggggaaa gcagacgtat acaattttca gctgaacaat
     4501 tggcaaatac cctaagcctg gctcaacaac aagccatttt agaaagcagc acccttgggt
     4561 taaaaataga taatactagt taccaaattc tgaaatttca ggatcaggcg ggatggaatc
     4621 ccatatcgaa caaaggaatt tttaaactct actattttcc caaaaacgtt gtgattagcc
     4681 ttaaaacaaa tcaaaaacat acccggggga accctgcaat cataatagca ccatctggtg
     4741 acatgacccc ttttactata gaatttggca cagataagga aaaatggata gccaaactta
     4801 caggcagcca taatggtgat ttggattata ccatggcaaa atctcaatga tgccgaaaac
     4861 taaattagtc tccggattta ctttgataga agtcttattg gctcttactg tcattgcaat
     4921 tgctttaaca gcgcttttaa aagcaaccgc tcaaaatata gacaacacgc atagaattaa
     4981 agaaaaaaca attagccatt gggtagcaat gcaaggggta gcaatgatcc aattaaatct
     5041 gctgcgcact tctcaaagcc aagaatcaac acaagcaacc accatgctgg ggcaaaagtg
     5101 gtactggaga gctaaaatta gccagacccc tataaaacga atgcaacaga taaccatttc
     5161 agtcagttcc aagcaagcag gtccctttcg agaagaactc attgcattca ggtacttgcc
     5221 atgattaaaa ataaaggatt tacactgatt gagatattaa ttgctcttac tgtttttgct
     5281 atattagcaa ctattacctc ctcaacctta tactacgcat tcaatactcg aacccgagtt
     5341 aacgcgcagg ctgaacgctt aaatgcactg cagctggcaa taagtatcat tcagcaagat
     5401 acttcccaaa cagtggaacg tgctataaga ggtaatgaaa tgcgcctgtt tccaatattt
     5461 gttggtcaac ctcaatacct tgaactaacc agggatgggg tggtaaaccc caatagtgtt
     5521 gaaaaacgca gtaccttgga acgagtcgca cttatttgcc aggatagcaa attactgcgc
     5581 cgcacatgga atactttaga tcctatagac agaaatatct ataaagacaa agtacttctg
     5641 gataatttgt ctgaatgtca tttcaactat ttaaatcaaa atttacagct tctctccgag
     5701 tggcgagaac aggcagtcaa tcaaaatcag caaagagaac ctcttcccaa agcaatccaa
     5761 atgaatctca ctttaaagga ctggggagat atgaacatgc tttttatcat acccggagca
     5821 ctctatgttt atgagtagtc cacttccaaa atccattgcc agttcaaaaa tgcaaggaag
     5881 cgccctctta actgctctat ttattatgac attggttgct ataatcgcca ctgccatgag
     5941 tacacgagtg caacaggaca tttacagaac aaggctagtg attgctcagg ataaactata
     6001 tcttgcctct caggcagtca ctttctgggc tctcaatgaa ttacttgata aaaacaaccg
     6061 ctttacaaaa accaaccagt tagggatggt agcccaatac cctaaaaata tggaatccat
     6121 atacaatcaa gtacaattaa gtggtggaat ttttgattta caagcgcgct tcaacctcaa
     6181 taacctgatt gagaaaaaat ccattcccac tttaatgcac ctgatcagtc acgcctactc
     6241 gaaggcagcc ggccaagaac gtgctaatat agccttggga gtaaagcatt ggttgcttgc
     6301 ttatgatctt ggccgaggag aagatattta tacctcttat tatctctctc agaaaccacc
     6361 ttactatccc agccatcaac ttataaaaag taaatctgag tttcgattga taaaagatgt
     6421 aagtgcaccc gcttatattg cccttgaacc ttttatcaca gccttgcctg aatcaacgag
     6481 cattaacatt aatactgctc caaagcaaat attgatgtca ttaggagatg gattaagtga
     6541 cgcccaggca aatgaattaa taactgctcg gggggaaaat ggtatcactg atctgaaaga
     6601 gatcaacgag ttattaaaaa aattcaatat tcccagtgac caaataacta ttgaaagtca
     6661 atattatctc agcgttgcct atgcaaagaa tgatgaattt agtttggttg tgtattcttt
     6721 gttaaaacgc agtcgtgaca ggaggggtaa gttattaaca agcgtcatta gagagagtat
     6781 taataatttt tgacttaagc tcaaatgttc caaacaatat gaatacgcca gaatccaact
     6841 tttagttaat gggttgccaa ccgattttta tttttaccag gaatgagtgc ttttagtttc
     6901 tggaaaatgc aattcttgct gagatttcat cctccactaa tcgatatatt ttaatataat
     6961 atgatcattt actatactaa tagatatcat caaactcatt tggatttcac gatgcactta
     7021 gttttatttt ctaactgttt acgaggaaaa caatcaacat taatgctcgc ttcgcttttt
     7081 aacataccct tttactaact acttgttatg attaatatta ttaaaaacag aaacaagaaa
     7141 aaccatactg cttccactga gttgaaatca gaaattgaga aggagcaggt caaactgctt
     7201 tttgatcaag ttggtatggc tatatcaggt gaagctttag cagtaactat actacctatt
     7261 gcattatggt ctgttacaaa tcatgagcta cttattattt ggatgatttt tacttatgtt
     7321 ttttcagatc tttataaaag catgctgctc ttctattaca ataagcaacc aacattattt
     7381 tcaaccgaaa aatggttgtt cctttttaac ttgggtgttg taagctctgg tttagcttgg
     7441 ggatttgcca gttcgattat gattccagcc acaagtacat tgcatcaaat ctttgtcgtt
     7501 tttcttatta caggagttac agcagcagcc aattcactct attcacctgt gcgattatcc
     7561 tattttttat ttctggtcac tgcttttgtt cctttcacta tatggttatt ttcaaaagga
     7621 caagtttatt tattgttggg gttttgcggc attatttata tgggaattat gttgtttatt
     7681 tcatattact caaacaggat tttgattaat accttaaaaa tgcgctttaa aatctctaat
     7741 ttaaattcaa ccaaagatat tttagaaaaa aaagtagtgg acagaaccaa agaattggaa
     7801 aaaatcctgg ctttaaccaa atcaacttta gagtcaacag cggatggtat tttagttgtt
     7861 gatctcaaag gtaatattga attctgcaat cagcaatttc tcaagatgtg gggaatttct
     7921 ccagggttta ttgacagtca taatgacaca gaaacaattc aatatgtatt gaatcagctt
     7981 aaaaatccgg aagaattttt aaaacaaatt aaagaactat acaataatcc aaagcaggaa
     8041 agctttgatg aattgcattt caaagacggt aaaatctttg agagatactc acaaccacat
     8101 tggttagaca ataaaattgt aggacgtgtt tggagtttta gagatgtgac cttgagcacc
     8161 agattagcct atcaagccaa tcatgattct ctaactggtt tacccaatcg tacagctcta
     8221 tacgatcgcc tggatcacgc gattaattac tgtaaacgat taaacacctc attatcagtg
     8281 ttatttctgg atattgataa ttttaagtta attaatgaca gcctgggcca tgatgccggt
     8341 gatatattat tgtatgaatt ttccaggcgt cttaaaggat gcatacgtca tagcgataca
     8401 gttgctcgat ttggcggtga tgagtttgtt ttgttattga tgaccactga acctaaagat
     8461 gttattttag tagcgcaaaa cattttaaag gctgtagcgc aacctatcaa attgcctacc
     8521 caagaagtca tagtgacgac aagtatagga attagccttt tccctaaaga tggcattgat
     8581 gcaagcacct tactcaaaaa ttccgatacc gcaatgtacc tggctaaaaa ggaagggcgc
     8641 aataatttcc aattttataa taattcgatc aatcaacaat cgctcaaacg ccttgagata
     8701 caaacacaga tacacaatgc aattaaaaat aatgaatttt ttattcttta ccagcccatt
     8761 tataatttac gtagcggtga attagtaggt gccgaagccc tgatacgttg ggggcatcct
     8821 gcaataggac tcctctctcc ggatgaattt attcctattg ctgaagaaac cgggttgatt
     8881 aaccaagttg gcgagtgggt gttaagaaat gcctgtattc aaaataaaac ctggcagctt
     8941 atggggttac cgcatattag tatttccatt aatgtctcat ggagacagtt aaaaaatagc
     9001 aattttatcg agattgcaga atctgtatta aaagaatcta gactggatcc tcagtatttg
     9061 gaaattgaat tcaccgaaag tactatgata caaaaatcaa cgcccattga ttcgacgtta
     9121 ataaaaattt cccagttggg tattaaaatg gcaattgatg attttggtac cggttactca
     9181 aatttaagtt atttaaagac tttccccttc aataaactta aaattgatcg ttcatttatt
     9241 caaaactgca ccactaaccc aaatgatgcc tccatagttg aagcaattat tgccatggcc
     9301 catagcctgg aactcaaagt attggcagaa ggagttgaca cgattgataa agcgtttttt
     9361 atgcatcaac atggatgtga tgaagtacaa ggtttccttt atggcctacc tgttaaagct
     9421 gataagtttg tgaaattaat gcaaaaaact aaaatccgcc attcaaaatt tattaataaa
     9481 acataagatt gttgtttctt ttattactat aaagaaaata ctacatacta ctcatcaatt
     9541 aaactagctc acttaaacca ctattttaaa cacacaacaa attatcaagt tacaactata
     9601 atcttctgga ttcagttttt aactttagtc ctcataattc tctttgcttt ctaatgggcc
     9661 caaatccttg atcattttct ctgcttccaa tttttcaggt tcggtcaact tggcttccaa
     9721 agatttaagt tgctttgctg cttcctcatt tttattttca gcagcaattt ttaaccaggc
     9781 gtaagcttta accaagctct tgttgactcc taatcctgct aaatacatat accccaattt
     9841 ggcttgtcca acaggatttc cctgatgtgc tgacttacca aaccagtaag ctgcctgttg
     9901 gtaatcttta tcaacacctg taccagttaa taacaattgt cctaattgtg cctgtgcttc
     9961 aggttgtccc ttttctgctg cagattggta ccaataagct gctttcacag gatcagcatc
    10021 tacaccatac cctttaagat aatagtaagc taaataggtt tgtgcgttga catgtccatg
    10081 attggctgct tgtgaaaacc agtaaaaagc ggctttatca tccgcactaa tcccaccttc
    10141 ctggcctgta tacaatagac ctagactgta ttcgcccttt tcatccccct gctctgccgc
    10201 cttttgatac cattcaaatg ctttttttac atcttttgga gttcctttac ccatcatgta
    10261 ctgataaccc aaattcactt gtgccttgct atagccttgt tctgcggcct ttttaaacca
    10321 ggtgaatgct ttgtcatcac tggcagctac accatctcct gtggcataca ttaatccaat
    10381 atttctttgt gctatcggat ttccttgctc tgctgccttc atataccatt tgaaggcctc
    10441 agcaaaatcc tgagataccc ccttacctgt atcgtacata aatcccaaac ttaattgagc
    10501 caatgcatta tttttatcag ctgccttttg ataccagttt ctggcttctt cgtaattagt
    10561 tgtcacacca tatccgtatt gatacatgcg ccccaataaa tacatggctt cctcattacc
    10621 ttctttggca gcctgcatta aatgtggata ggctgtggtg tagtcgccat tttcaaatgc
    10681 tgcaaaacct actaattcat cagcaaaaac actactgcta attgctaata acagagccaa
    10741 gacaaaacct tgcatatctg ctccatttca atctcctatt attttaagag tagaataaag
    10801 aaataacaaa gcaagttatt gatcaattta aaacatcacg atccaaaata agtcccaaat
    10861 aattttaaac acccaccaaa aaacgcttaa taattgttta atatttatgc gatataatac
    10921 caatataaac attaagatca tttattaatc tagttatttg tttttatcta aaggagaaca
    10981 gatatgtctc gctccaaaga tgaaattcta aaagtaaata attctttatt cggtattacg
    11041 gtgcaaacat ggggaactaa tgatcaacct agcaatggaa tgatgaattt tgctgatcag
    11101 caattttttg gcggggatgt tggacatgct tccattaata tgaaattacc tgtgactgat
    11161 aagacaaagg aatggataga aaaatattgt tattcacaaa cctatgatca atttaagcaa
    11221 gtaaaaggca atgaagataa aacctatgaa gaatatctga agaccgcaaa acgacttata
    11281 cctgtagaat taaaaacgca agttacgagg aaagcccaat acgattcaaa tgggaacctt
    11341 gtagccaccc acgaaaaagc ttatgaacaa acttattttg acatcgattg gagttggtgg
    11401 ccaggaagat tacaaaatac agaagatgac atggtttggg aaagagaggg aaaacatgtt
    11461 gaatacgttg agaaatggaa ggaatatttg caacctgaac aaagggtaca taggggtaaa
    11521 ttaggctcca gaaaaatgga ttatgcccct acagcaatta tacatcaaag ggatatccct
    11581 acaagtgagt tagagagaat tacacgtgat cataaaattc atacaatcga agaaaaacta
    11641 aatgtagtaa agctactaca aagtaaaata gatgaaatgc ctcatacaaa aatgagccct
    11701 tcaatggagc taatgtttaa aaacttaggc attaatgtcg aaaaattact agatgaaacc
    11761 aaagacaatg gagtagaccc aacaaactta gaagcaatga gagaatattt aacaaaccgc
    11821 ctgacagaac ggaaattgga attagaaact gaactctctg aagctaaaaa agagatagat
    11881 tcagcacaaa ttaaaagtaa agttgaagat gtatattatg attttgaata taagttaaat
    11941 caagtgcgta aaaaaatgga agaaatagac actcaacttg aaaaaatgga tagtttactt
    12001 caaaagctag agggcaatac atctgaatcc ttgccttata cagctgaaat cgacgaactt
    12061 ttatcagcct tacctttttt aaaggaggac ttaaagttag aaaatggtac attgtcaccc
    12121 aagtctattg ataatttgat cattcatatt gatgaattaa aaaatgagct tgctatcaaa
    12181 aaagagaaaa aaaatgagag ggatctgaat ttaattaaaa aatacgaaga attatgtgag
    12241 caatataaag atgatgaaga aggattggaa ggagctctat gggaagaagg gattgacgta
    12301 gaggatgttg attccgccaa aaaaaatatg tccaaacctg atccagaaat ccaaaaatta
    12361 accgatcttc aggaacaatt gagaagtcat aaagaatcag gagtaaagtt atcatctgaa
    12421 cttgaagaaa ctctcaattc ttctgtaaga atgtggaaaa ccaaaattga ttctccatgt
    12481 caaattatat cagaatcttc agtaaaagca ctcataagta aaataaattc aactcgtccc
    12541 gagcttgcaa aagaaagaga acagttacca gcgcaagaag aaactctacg tcaagaagct
    12601 aaaaaggcag aagaagaact atttaaaata caagagtttt ctcaatttta cagtgaaaat
    12661 tccagtgctt atatggtaat tggttcacct cctcatcatc aagtatcctt acctttagct
    12721 gtcaatggta accgaggttt gcatcctgaa gctatgttaa gaaaaatgca tgaacttgta
    12781 gctgggccag aaaaaaaaca attcaattta cataccaata attgctcact tacttcaata
    12841 gaagtattat ctgctggagc agaacatgat ccactattac attcaataat gggaacacga
    12901 gccttaggtt tttttggaac gccacaacag gtattagaaa acgccaaatt aacaagtaaa
    12961 accataaatg aagggaaaaa aagtaatatt tttacatcat tagctactgc gaacccatta
    13021 gacagagcat tgggatacgc catgtcaata tacatggatc ctgaagcctc agaagcaaaa
    13081 caaaatgcag gcctagctct cggcgtactg gttggtttag ccaaaacacc aggtattatt
    13141 attggct
//
LOCUS       DAXLDT010000086        12961 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXLDT010000086 DAXLDT010000000
VERSION     DAXLDT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.086"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(252..1925)
                     /locus_tag="ACF2SP_001966"
     CDS             complement(252..1925)
                     /locus_tag="ACF2SP_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HFD2359746.1"
                     /translation="MKTRIVSLLVVSMVSANLQAASENIIWQAGDNNLKKYNFSILQD
                     KKGFISITNKITPLNYQLKPVDHEGNRDSNDIRYQVYYKNIPVWGHELILHKASKSKV
                     FLTGVDVSEIEKDVHQTDGKLSAADIESQIIKHNNNSIVYKNIKKIIYLDSRKKAYLA
                     YHLSMYINDPLHFVSAPNFIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLF
                     QHGDSMKDIPSLGKFEVMIKGKNCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNI
                     PAFSYPCSKESQYININDGDTSPANYSFSSVNDAMYFAGVTLEMYKEYYGVEKPLGDD
                     LPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNF
                     TRLHSNLVYNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPI
                     RYMDYPTKDGHSIEHFDDYHDDLDVHLTSGIFNRAFYLLAHQPNWSVRKAFQVMVDAN
                     IKYWTSGTSFEAASCGVIQAAIDRQYNKQDAINAFKEVGVICPVIGLIN"
     gene            complement(1967..2890)
                     /locus_tag="ACF2SP_001967"
     CDS             complement(1967..2890)
                     /locus_tag="ACF2SP_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HFD2359747.1"
                     /translation="MISNKCDVVILTQKDFIDPADITPYIQNVLTEDRLLTEALEQKG
                     LKVKRTHWDNRQYNWSETRYALFRATWDYFHRYDEFQLWLSDCSKRIQFINPISIIQW
                     NIDKHYLSDMQQKGINVPPTLFLKKGELNILNNLMKKSKWKKAILKPAIAGGARHTYL
                     FDQDSADHYQDVFESLISNESMLLQEFQENIVNKGEVSFMVFGGRYSHSILKKAKSGD
                     FRVQDDFGGTVHPYEASQEEREFVENVIAQCDELPVYARVDVMWDNNNKLCLSELEMI
                     EPELWFRESSQAASAMADAVCEHISGVSEYK"
     gene            3072..3839
                     /locus_tag="ACF2SP_001968"
     CDS             3072..3839
                     /locus_tag="ACF2SP_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HFD2359748.1"
                     /translation="MFKNAIVRTPSSTMINGLTTSADLGKPNYELALIQHQNYIKALS
                     TCGLEVTVLPVLEQFPDACFVEDVSLLTEQFAVLTCPGAESRRDEVQEIEPSIQVFFK
                     DRIFRINPPGRLEAGDVLRIDNHFFIGLSERTNKEGAEQLIYLLNQHGYTASVIQLKK
                     FLHLKTGVSYLNNDYVLVSGELINHQAFGHLKQIEVSSEEAYAANCIMINETVLLPKG
                     CPKITDHLSELGFSIIELDMSEFRKLDGGLSCLSLRF"
     gene            3839..5155
                     /locus_tag="ACF2SP_001969"
     CDS             3839..5155
                     /locus_tag="ACF2SP_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2359749.1"
                     /translation="MKSRLSVLSLALITVCSVDSIRNLPAAALAGDQLFYYFALALLF
                     FLLPCAIVAVWFSRQSEQGVYGWVKQGLGSQWAFIAIWFQCIQNVLIYPTLLSFIAGT
                     LLYSISPDLVQNKSMLFLIIIFLIWSLTWINLKGIHLSSRFNSYCSITGLILPFIIIL
                     FVGLYWWITQVKPGTTVLPPAESYSWTSLTAIILSFCGIELAAVHARDSKQGAFPKAI
                     AISVITIFLTMLFGSIVLAMIIPPEQLNFISSIPQLIQLFFNKIGYGYFAFIINGLIA
                     IGCIGAANNWLIAPIKGLEFAINEGFLNRQLSQVNKHNAPFRLLVAQAFCVSIISALF
                     LIIPSINASYWIMLNAATQINLLMYLLLFISAIKIVSSKPILSKLNIMIFAYLGLAGS
                     SITLIVSLTPPPSLAISSKLIYALLGAIFLVIMTLIPIWRKPKVLT"
     gene            5291..6328
                     /locus_tag="ACF2SP_001970"
     CDS             5291..6328
                     /locus_tag="ACF2SP_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HFD2359750.1"
                     /translation="MKLTRNMIAQAVQAKIITEEQAKNLIEFIKNLPEQTPGFNLTNV
                     LYYFGGLIAIGAMTLFMNLGWELFGGWGIFYLSICYALLGLGLCSQFNGKGYVIPAGI
                     CATFVICLTPLAIYGLQKGMGWWPDDSVYHDYHVYIKWHWIFMELGTLMVGIILAWIY
                     RYPFMIMPIAVTLWYMSMDIAAMLGDGQFDFELRAMVSMYFGLITVLIAFWVDVRSSQ
                     SADYAFWLYIFGVMAFWFGLSCQSSDSEISKFIYMCINLFMILTGVILNRKVFVIFGA
                     LGCSYYLGHLAFQVFKFSMLFPVALTAIGFGIIYLGIIWQRNEAKLTKELRSILPKPL
                     RDLLEARDSMD"
     gene            6467..7948
                     /gene="legL3"
                     /locus_tag="ACF2SP_001971"
     CDS             6467..7948
                     /gene="legL3"
                     /locus_tag="ACF2SP_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL3"
                     /protein_id="HFD2359751.1"
                     /translation="MLTLSLAKCINATKNGAFAIKDLDLSGPLSYEDVLFLAEYLKVT
                     PVTDLDLSMTITRENSKALWDLSQVIAKNSLLQKLTFEVHLDFDFYFKHNLPIGWLDF
                     IDDYRLKKIAAPLHETLVNMLKNKPALNKLTIDFLAMETPFHNKHLDQLARSINKIPL
                     NYLNLGLSVTKETQVPLFKTKEPNQTLEFLILRGFNSGRNTLKYLPQTAALKMLAIDD
                     MQFEEHDLKLLANIMKSNSSLVALILGKTNLGQVNSPDVLTWLSACPNLNSLILIDNN
                     LSRLNVDSLCELLANNLQALKKLDLSKNAYMGDDAIKIAKALTFNAHIETLVLDDNYI
                     ENEGLQAIINLVKTNKNITSLSISNTCRYTPSNEIIHSLCDLLRDPGCQLKELKFNQD
                     ITLAQYLMLQHALNSNKTLEYIDFNFRNQNILEAILGKNNSSDLNENEFSSDNPKEEK
                     APNTLNTDPTSKSAPLFFISPKSRHEENENELSSLSSHSVHPH"
     gene            complement(8042..9160)
                     /locus_tag="ACF2SP_001972"
     CDS             complement(8042..9160)
                     /locus_tag="ACF2SP_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1661 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359752.1"
                     /translation="MNTVPLKPQRLLSLDVFRGMTIVLMIIVNGQAAIDPYPIFEHVD
                     WNGCTLADLVFPFFLFIVGLTSVISLKNQMERKAKTSLYSAIIERSVVLFLLGLFLNV
                     FPHPIEFDSIRIYGILQRIAVCYLISAFIYLNTSIKTQFFIFLVLLLGYWIIMTQVPV
                     PGYGANQLTKDGSWVSYFDQLFFSASHLYEKTYDPEGFVSTFTSIATTLSGVLAGSLL
                     INPCNQFKKFYLLAGVGMLFLLLGWLWNMSFPINKNLWTSSYVLWTSGLALLVFAFCY
                     LLIDRLGVKKWSVFFKIFGMNALFAFVFHVLLLKLQYAFKITTPDGSKMALISYLKDY
                     FFGGFSNHNAALLYSICFLFLNFLVVLILYKRNVFIRI"
     gene            9321..9917
                     /locus_tag="ACF2SP_001973"
     CDS             9321..9917
                     /locus_tag="ACF2SP_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359753.1"
                     /translation="MFSKKERTLLFANNLWYLSEGMLGPLFGIFCERFNSSPLDISWV
                     WSLYLISVGIVVIGLGYISDFIHKEKLLVAGYALQTIFTFCYIFITSKKQLALLQIGL
                     GISGGLSTTTWYALYARYEDKTHEGLTWGLANGLSSIYTGIALLCGGLIIAKTSFDVL
                     FIIMGCIQFLATIYVSKLIFIEHPKIIRRFRNPNLAKN"
     gene            10081..10593
                     /locus_tag="ACF2SP_001974"
     CDS             10081..10593
                     /locus_tag="ACF2SP_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359754.1"
                     /translation="MFESFRKCLSAISMRAPYASTPGHRNAQRANAGSEVLGTTLETF
                     DPAASIPTKLAQTLISIYSLFRFDTHVSEKLIHLLQGSIAATQMGLGIALLFTGTECE
                     EYTDADLCKAIFLLQLLYRGTLLVGWAPSEFSKDPYAEPKVVSEDKAESDAESEEENE
                     DEESRNSASV"
     gene            complement(10652..12958)
                     /gene="pbpC"
                     /locus_tag="ACF2SP_001975"
     CDS             complement(10652..12958)
                     /gene="pbpC"
                     /locus_tag="ACF2SP_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HFD2359755.1"
                     /translation="MRKVIKYLSIILGSLFVSGFLLLFFSPKPSLLEEFNFSKAVYDD
                     NQQLLRLILSDDDKYRLYVPLSQIPKELMEATLLQEDQYFRWHKGVNPFSMVKAIWQT
                     YVVKSRRIGASTITMQVARIRFGINSKKISGKLWQIIRALQIERHYDKDKILEAYFNL
                     APYGGNIEGVGAASLIYFNKPVSKLGLPEALTLSIIPQNPVKRTSKNSELKKIRNRLF
                     ARWLNQHPQDQEKQPLFGLPLVMQSNQNLPFLAPHFVNMVLKENPGLSQSFSTTLDYR
                     LQLIVERITRHYLARKKMFGVHNAAVLLVDSRTMSIKALMGSADFFNNKISGQINGVE
                     TKRSPGSTLKPFIYGLALDQGLIHPNTVLKDVPQSFNGYNPENFDYDFMGPIKAKDAL
                     VLSRNIPAIELSNQLTNPTLHKLLEQAHVTQLRSESYYGLSLNLGGVELTMKELVGLY
                     AMLINDGVWYPINSLKGVPRQKGRRLLSTEASFLVFDMLKNTPMKESNNEGSSQLPIA
                     WKTGTSSGYRDAWTVGVFGPYVLAVWIGNFDNQANPAFIGKEIAAPLFFELIDAIKHE
                     RGPIARVEKYPEHMHLEKIEVCKASGMLPGRYCTDTEWTWFIPGKSPIKTDTIFREVA
                     INGKTGLRTCHIDENTRFEVYEFWPSDLLRIFRRAGIQRHTPPFFEPGCALSGNSGIS
                     PQITSPQAGVSYIVHANTQSNKIIPLTAVTDAGIAHLYWFINESFVAETKSDEPYLWQ
                     AKPGKYVVRVVDDRGLSDARDITIQMVS"
BASE COUNT         4053 a         2476 c         2260 g         4172 t
ORIGIN      
        1 ctctttggca gaggctattt tctgcctggc atgtattctc tttacttcat ccatcattga
       61 ctcctttaat gcgaattcag atccatatat aaagacaact tattgtaaaa taatccaacc
      121 cgcttaaaac aaataggaat tttcttaatt gtctgaaatt aaaaagaatt ggttgtgagc
      181 tggaaagttg aattaatatc attatctctt tttatcagca atcagtatcg aagtcacgtg
      241 aattgctgta tttaatttat gagacctata acagggcaaa tcactcctac ctctttaaaa
      301 gcattgatgg catcctgctt attatattgc ctgtcaattg ctgcttggat aacaccacaa
      361 gaggctgctt caaatgaagt ccctgatgtc caatatttta tattggcatc taccatcact
      421 tgaaatgcct ttctcactga ccagttcggt tgatgggcta aaagataaaa agccctgtta
      481 aagatcccgc tggtcagatg aacatccaag tcatcatgat aatcatcgaa atgctcaata
      541 gaatgaccat ctttcgttgg atagtccata taacgaattg gctcaccact tatagtagcc
      601 tcacgcccta tagaccaatc ttcaccatcc caataccatg gaaaatcttt acgcaagtaa
      661 tcctgcatag caatcgatgc catatccgag aatgcttcat tgattcctcc ggattgccca
      721 ttatagacca ggttagaatg taatctggta aaattatgag acaactcatg cgataaagtg
      781 ctctgtgccg gagctgttaa ttgagtatcc ccatcaccaa tcacaatttg ttgatgcatt
      841 aagtacaatc cttttatttt aatagaaggc acagcaaagg cattatcaaa atttttaata
      901 tgtgtatagg ctcgcaaagg caagtcgtca cctaaaggtt tttcaacgcc ataatactct
      961 ttgtacattt ccagtgtgac tccggcaaaa tacatagcat cattaactga agaaaaactg
     1021 tagtttgcag gtgatgtatc tccatcatta atattgatat attgtgattc tttgctacat
     1081 gggtaggaaa atgctggtat atttttatag atttccacaa tgctaagaac ggggaaggat
     1141 ctcttgtcca aatcagtata cgcagcatta atgaccctta cctcagggct ttctacaaca
     1201 caattttttc ctttgatcat tacctcaaat ttgcccagag aaggaatatc cttcattgaa
     1261 tcaccatgtt gaaacaatcc cgaccgatag ggaagaatga atacattacc ccccaagccc
     1321 tgaccaacct tcttatgagt taaatcatcc cagcttttta aaatctcacc agaattggca
     1381 tcgataataa aatttggagc agagacaaaa tgtaatggat catttatata catcgataag
     1441 tgataagcta gataagcttt tttacgtgaa tccagataaa tgattttttt tatatttttg
     1501 taaacgattg aattgttatt atgttttatt atctggcttt caatatcagc agcacttaat
     1561 ttcccatcag tctgatgaac atccttttca atttcagaca catcgacacc ggtcagaaaa
     1621 acttttgact tgctggcctt atgaaggatt agctcatgac cccaaacagg aatattttta
     1681 taataaacct gataacgaat atcgttagaa tcacgattgc cctcatgatc aacaggtttc
     1741 agctgataat ttaacggcgt gattttatta gtaattgata taaatccttt tttatcttgc
     1801 aagatactaa aattgtattt ttttaaattg ttgtcgcccg cctgccaaat aatattttct
     1861 gaagcagctt gcaaattagc gcttaccata gaaacaacta ataatgaaac tatccgtgtt
     1921 ttcatatttt ttccctgtct ttgactattg ttgtgtctta cagcgtttat ttatattcac
     1981 tgacccctga tatgtgttcg catactgcat cagccatagc agacgcggct tgactgcttt
     2041 ccctgaacca aagctcaggt tctatcattt ccagctcact caaacacaat ttattattgt
     2101 tatcccacat gacatcaact cgtgcataca cgggtaactc atcgcattga gctatgacat
     2161 tctcaacaaa ctccctttct tcttgagatg cctcataagg atgtacagtc ccaccgaaat
     2221 catcctgtac tctaaaatcg ccagattttg ctttttttag tatagaatgg ctatatctac
     2281 ccccaaacac cataaaagag acttctcctt tatttacaat gttctcctga aattcctgga
     2341 gtaacatcga ctcattgcta attagtgact caaatacatc ttgataatga tctgcggaat
     2401 cttgatcaaa taaataagta tggcgtgctc cgcctgcaat tgcaggcttt aatattgctt
     2461 tcttccattt cgatttcttc attaaattat ttaagatatt tagctccccc ttttttaaaa
     2521 ataaagtagg agggacatta attccttttt gctgcatatc actcaaataa tgtttatcaa
     2581 tattccactg aataatagaa atcggattta taaattgaat tctttttgag caatcactta
     2641 gccagagttg aaactcgtca taacgatgaa aataatccca ggtggcacga aacagtgcat
     2701 atctagtctc tgaccaatta tattgccgat tatcccaatg agttcgttta acttttaatc
     2761 ccttttgctc cagagcttcg gttaataatc gatcttctgt aagcacattt tgaatgtagg
     2821 gagtaatatc ggccgggtct ataaaatcct tctgtgttag tatgacgaca tcgcacttgt
     2881 tagagatcat gatagaaaca atacgtatca aatgatacaa gaatacttat ctttttaaac
     2941 taaatctata tacccctatc gcaaatcaac cagcgaattt aatcaagtta acaatgattg
     3001 cattttgaag actctttgtt taatctctgg ctgtgtgtgt attggaaatc acttatttaa
     3061 ggacatgatg aatgtttaaa aatgctatag taagaacacc atcttctaca atgatcaatg
     3121 gactaactac atcggctgat ttggggaaac ccaattatga acttgcatta atacagcatc
     3181 aaaattatat caaagcacta tcaacatgtg gtcttgaagt gacagtgctt ccagttctag
     3241 aacaattccc ggatgcttgt tttgtagaag acgtgtcatt attaactgaa caatttgctg
     3301 tcttgacatg ccctggcgca gagtccaggc gagatgaagt tcaggaaatt gaacccagca
     3361 ttcaagtatt ctttaaagac aggattttca gaataaaccc gcctggcaga cttgaggctg
     3421 gagatgtgct tagaatagac aatcattttt ttattggtct ctcggaaaga actaacaaag
     3481 agggggctga gcaactaatt tatttactga atcagcatgg ctatacagcc tcagtaattc
     3541 agctaaaaaa atttctgcat cttaaaacag gagtgagtta cctgaataat gattacgtat
     3601 tagtgagtgg agaattaatc aatcatcaag catttggtca tttaaaacaa atagaagtaa
     3661 gttctgaaga agcgtacgcc gctaattgta ttatgattaa tgagactgtg ttattaccca
     3721 aagggtgtcc taaaataacc gatcatttat ctgaactggg gttttcaatt attgaactgg
     3781 atatgagcga atttagaaag ttagatggtg gtttaagctg cttgtcgttg agattttaat
     3841 gaaaagtcgc ttatctgttt taagccttgc cttaatcact gtttgctcgg ttgatagtat
     3901 tagaaatctt ccagcagctg ctttagcggg ggaccagctg ttttattact ttgcattggc
     3961 cttgctcttc tttcttctcc cttgcgccat tgttgcagtt tggttctcaa ggcaatcaga
     4021 acaaggtgtg tatgggtggg taaaacaggg tctagggagt cagtgggcat tcatcgccat
     4081 ttggtttcaa tgtattcaaa atgttttaat ttaccctacc ttattgagtt ttattgctgg
     4141 caccctgctc tacagcatct cccctgattt agttcaaaac aaaagtatgc tttttctaat
     4201 tattattttt ttaatttgga gtttaacctg gattaattta aaaggaattc atctctcaag
     4261 tcgttttaac tcttattgct ccataacggg attaattctt ccttttatta ttatcctgtt
     4321 cgtggggttg tactggtgga ttacgcaagt taaacctggt accacagttc taccaccagc
     4381 cgaatcttat tcctggactt cgcttactgc aattattttg tcattttgcg gtattgaact
     4441 agccgcggtt cacgcacggg atagtaaaca aggggctttc cctaaagcca tagctatttc
     4501 agttattaca atctttctga cgatgttatt tggttcaatc gtattggcta tgatcattcc
     4561 cccagaacaa ttaaatttta tcagcagcat tccccagctc atacaattat tttttaataa
     4621 aattggttat ggatattttg cctttattat taatggattg atcgcaatag gttgtatagg
     4681 tgcagcaaat aactggctca ttgctcctat aaaaggattg gaatttgcaa taaacgaagg
     4741 gttcttaaat aggcaactca gtcaagtgaa taaacataac gcacctttca gattgttagt
     4801 cgcacaagct ttctgtgtat cgattattag tgctttattt cttatcatac cttcgattaa
     4861 tgcatcttac tggatcatgt tgaatgcagc gacacaaatc aatttattaa tgtacctctt
     4921 gctcttcatt agcgctatca aaatagtttc ctcaaaaccg attttaagta agctaaatat
     4981 aatgattttt gcttacctgg ggttggcagg cagtagcatt actttaattg taagtttgac
     5041 gccaccgcca tcactcgcca ttagctccaa attgatttac gcattgcttg gcgcaatttt
     5101 cctggtcata atgaccttaa ttcctatttg gaggaaacct aaagttctta catgaaaaga
     5161 aatatccaat cctgatttgc aaattcaaac gcaagtttca tactctcagc tataattgag
     5221 atattaatca ttagttattg taaaatcagg ttaaatttta gcaggaattc agtactcatc
     5281 ggagatgatg atgaaattga cacggaatat gattgctcag gctgtccagg ctaaaataat
     5341 tacagaggaa caggctaaga acctcattga attcataaaa aatcttcccg aacaaactcc
     5401 aggatttaac ttaaccaatg tcctttatta ttttggagga cttatagcta ttggtgcaat
     5461 gactttgttt atgaatcttg gatgggaact gtttggaggc tggggtattt tctatttgtc
     5521 aatttgttat gccctactcg gcctaggcct ttgcagtcaa ttcaatggga aaggatatgt
     5581 cattcctgct ggcatttgcg ctacttttgt catttgcctt acacctttgg caatctatgg
     5641 cttacaaaaa ggaatgggct ggtggccaga tgacagtgtt tatcatgact atcatgttta
     5701 tatcaaatgg cattggatct tcatggagtt gggtacgcta atggtaggta ttatcctggc
     5761 ctggatatat cgataccctt ttatgattat gccaattgcg gtcactcttt ggtatatgtc
     5821 catggatata gcagccatgt tgggtgacgg tcaatttgat tttgagctaa gggcaatggt
     5881 ttcaatgtac tttggcttaa ttactgtatt gattgccttt tgggttgatg ttcgatccag
     5941 tcaatcagca gattatgctt tttggctgta tatattcggt gtcatggctt tttggttcgg
     6001 tttaagttgc caatcctccg acagtgaaat atccaaattt atctatatgt gcattaacct
     6061 cttcatgatt ctgaccggag tcattttaaa caggaaagta ttcgttattt ttggtgcgtt
     6121 aggatgttct tattatctgg ggcatctggc attccaagtg tttaaattca gtatgctttt
     6181 ccctgttgct ttgactgcca taggttttgg tatcatttat ttaggaataa tctggcaaag
     6241 aaatgaagcg aaacttacta aagagctccg atccatactt cccaaaccat taagagactt
     6301 attggaagca agggacagta tggattaatg aaatattttc tcccgtccta tcacattcta
     6361 ttactctttt agataaattg taggtaactc tttactttca agtacctgaa gtagaagata
     6421 gatgagttca tcattatttt ctttcaaaag gacttgagga gtaattatgt taactttatc
     6481 acttgccaaa tgtattaatg caacaaagaa tggggcattc gccatcaaag atttggattt
     6541 atcaggccct ctatcttatg aagatgtatt atttttagct gaatatctaa aagtcacacc
     6601 cgtaacagat cttgacttgt caatgaccat caccagggaa aactctaaag cgctatggga
     6661 tttaagtcag gttattgcta aaaattcact gctacaaaaa ctaacatttg aagtgcatct
     6721 ggacttcgat ttctatttta aacataacct gccaattggt tggttagatt ttatagacga
     6781 ttatcggtta aaaaaaatag cggcgccact ccatgagaca ttggttaaca tgctaaaaaa
     6841 caaacctgct ctaaataagt taactataga ctttttagcg atggaaactc cttttcacaa
     6901 taaacatctc gaccagttag cacgatctat caataaaatc ccgcttaatt atttgaattt
     6961 gggcctttca gtgaccaaag aaacacaagt accgctattt aagacaaaag aaccgaatca
     7021 aactcttgag ttcctaatcc ttcgtggttt taattcaggt agaaatacct tgaaatattt
     7081 accccaaaca gcagccttaa aaatgttagc tattgatgac atgcaattcg aagaacatga
     7141 tttgaagttg ctggctaata ttatgaaatc caactcgtca ctggttgctt taatccttgg
     7201 taaaactaat cttggacaag tcaattcgcc agacgtactc acctggctca gtgcatgccc
     7261 caatttaaat agtcttatac tcattgacaa taatctaagc aggcttaatg tggatagttt
     7321 atgcgaatta ttagcgaata atctccaggc tttgaaaaaa ctggatctgt ccaaaaatgc
     7381 ttatatgggt gacgatgcga taaagattgc caaagcgtta actttcaatg ctcatattga
     7441 aacattggtg ctggatgata actatattga aaatgaggga ctacaagcca tcatcaatct
     7501 ggtaaaaacc aataaaaata taacgtctct atccatttca aacacctgtc gatatacacc
     7561 ttctaacgaa ataattcaca gtctatgtga tttattacgt gatcctggat gtcaattaaa
     7621 agaactaaaa tttaatcagg atattacttt ggcacagtac cttatgctgc aacatgctct
     7681 gaactctaac aaaacactag aatacattga tttcaatttt agaaatcaga atattcttga
     7741 ggcaattctt gggaaaaata attccagtga tttgaatgaa aatgagtttt caagtgacaa
     7801 tcctaaagaa gaaaaagccc caaatacttt gaatacggat ccgactagca aaagtgctcc
     7861 attatttttt atttccccaa agagtcggca cgaagagaat gaaaatgaac tttcttcttt
     7921 atcgagtcat agtgtccatc cccattaggg acaacacaca ttacactcta ttataatttc
     7981 tcactggcga tatacacagt tggcgctgag cgatctgcga ggcccaacct accttacctc
     8041 tttatattcg aataaataca ttacgtttat ataaaatcaa aacaaccaga aaattcagga
     8101 aaagaaaaca gatagaatac agcaatgctg cattgtgatt actgaatcct ccaaaaaaat
     8161 aatctttgag ataggatatc aatgccattt tagagccatc aggtgttgtt attttaaaag
     8221 catattgcag ttttaaaaga aggacatgaa atacaaaagc aaataatgca ttcataccaa
     8281 atattttaaa aaaaactgac cattttttaa ccccaagtct atcaatcagc aaatagcaaa
     8341 aggcaaagac aagcagagcc aggccgctgg tccagaggac gtaagaacta gtccataaat
     8401 ttttattaat aggaaaactc atattccata accatcccaa taacaaaaat agcatgccaa
     8461 cccctgctaa tagataaaat ttcttgaatt gattgcatgg attgattagt aaactaccgg
     8521 cgaggacacc tgataaagta gtggcaatag atgtaaaagt acttacaaaa ccttcgggat
     8581 catacgtttt ttcatacaag tgagaggctg aaaaaaataa ctggtcaaag tatgaaaccc
     8641 aacttccgtc tttggttaat tgatttgctc catatcctgg aacaggtacc tgagtcataa
     8701 taatccaata tcctaataaa agaacaagaa aaataaaaaa ttgagtttta attgaagtat
     8761 tcagataaat gaaagcggaa attaaataac aaacagcgat tcgctgcaga atcccatata
     8821 tccttatgct atcaaattca ataggatggg gaaaaacatt caaaaataat cctaaaagaa
     8881 acaaaacaac acttctctct atgattgcag aatataatga ggtttttgct tttcgttcca
     8941 tttggttttt caggcttata acactcgtta agccgacgat aaacaagaaa aatgggaaaa
     9001 ccaaatccgc caaagtgcaa ccattccagt ccacatgctc aaaaatcgga tagggatcaa
     9061 tagctgcctg accattaacg ataatcatca atacaatggt catcccgcga aatacatcaa
     9121 gggataaaag gcgttggggt tttaagggaa cagtattcat gcgctattta aaatgaattt
     9181 ataaaaaact gagccccatt atcttatcac gagtggcaaa ttgtaatatc aaaaataaaa
     9241 attaaatttc taaaatctat atgaaaatct cttttaaact tgtatgttag tcatgattga
     9301 cttaacttga aattgaatta atgtttagta aaaaagaacg tacattatta tttgccaata
     9361 atttgtggta cttgtcagaa ggaatgttag gtccgctctt cggtattttt tgtgaaaggt
     9421 tcaactcgtc tcccttagac atatcatggg tatggtcttt gtatttaatt tcagttggaa
     9481 ttgtagtaat tggcttgggt tatatttcag attttatcca taaagaaaaa ttgctggttg
     9541 cggggtatgc gctccaaacc atatttacct tttgttacat ctttataaca agtaaaaagc
     9601 aactggcttt attgcaaatt ggccttggaa tatcaggtgg actctctact acaacttggt
     9661 atgctctgta tgcccgctat gaagataaaa cgcatgaagg acttacttgg ggtttggcca
     9721 atgggttgtc aagtatttac actgggatcg ccttgttatg tggtggtcta attattgcca
     9781 aaacatcatt tgatgttttg tttatcatca tgggatgtat tcaatttcta gcaactattt
     9841 atgtcagtaa gctcatcttt atcgaacacc ccaaaattat aaggcgtttt agaaatccta
     9901 atttggctaa aaattaattt taaaatctga aagccatgtt gaaatgtcaa atcaaatgaa
     9961 cattttttga tgtagattga tgctaaattc atcaagaaat actggaatca aaataacttg
    10021 tctgttaatg tccgctttta ttaaagatat cacgtattaa attgttataa ggatatgatt
    10081 atgtttgagt cttttcgaaa atgtttgtct gccatttcta tgcgagctcc gtatgcctcg
    10141 acaccgggac atcgtaacgc tcaacgcgct aatgctggat ccgaagtgtt gggcacaacg
    10201 cttgaaacat ttgatcctgc cgcatcaatt cccacaaaac tcgctcaaac tttaatctca
    10261 atttattccc tattcagatt tgatactcat gtttctgaaa aactgatcca tcttttgcaa
    10321 ggtagtatag cggcaacaca aatgggtctt ggcatcgctt tgctttttac tggaacagag
    10381 tgtgaggaat atacggatgc tgatctatgc aaggcaatat ttctattaca attactatac
    10441 agaggaacct tgctagtggg ctgggctcct agtgaatttt ccaaggatcc ctatgctgag
    10501 cctaaagttg tatcagaaga caaagcggaa tctgatgctg aatctgaaga agagaacgaa
    10561 gatgaagagt caagaaactc ggcctcagtt taattttaaa tcccttgctc cgattaggac
    10621 aagggatcac ttatacatca tcaaatatgc gtcaactaac catctgaata gtaatatccc
    10681 gcgcatcaga taaaccacgg tcatctacca cccttaccac atatttacca ggtttcgcct
    10741 gccagagata aggttcatct gattttgttt cagctacaaa actttcattg ataaaccagt
    10801 ataaatgtgc aattccagca tctgttaccg cagttaatgg gattatcttg tttgattggg
    10861 tattagcatg gacaatataa cttacccctg cctggggaga tgtaatttgg gggctaatac
    10921 cactatttcc cgacaaagca caacctggtt caaaaaatgg tggggtatgg cgttgaatcc
    10981 ctgccctcct gaatattctt aataaatcgg agggccaaaa ctcatacact tcaaaacgag
    11041 tattttcatc gatgtgacaa gtacgaagcc ccgtcttacc gttaattgcc acctctcgaa
    11101 aaattgtatc cgttttaata ggagatttac ctggaataaa ccaggtccat tctgtatccg
    11161 tacaatatcg accaggaagc atacctgagg ctttgcatac ttctattttt tccaaatgca
    11221 tgtgttcagg gtatttttca actcgagcta ttggtcctct ttcatgtttt attgcatcaa
    11281 tcaattcaaa aaataaaggc gcagcaattt ctttacctat aaaagccgga tttgcctgat
    11341 tatcaaaatt tcctatccaa actgccaata cataaggacc aaaaactcca actgtccagg
    11401 catctctgta tcctgaggaa gttcctgttt tccaggcaat tggtaattgt gagctgccct
    11461 cattattcga ctctttcatt ggagtatttt ttagcatatc gaaaactaaa aagcttgctt
    11521 ctgtgctaag caagcgtcga cctttttgtc gcggaacccc ttttaatgaa ttgattggat
    11581 accagacccc atcattaatc agcatagcat ataaaccgac taactctttc atagtgagtt
    11641 caacaccacc caaattaaga gacaagccat aatatgattc ggaacgcaac tgagttacat
    11701 gcgcctgttc aagtaattta tgtaatgttg gattagttaa ttgattggaa agctcgatgg
    11761 ccgggatatt acgactaagt accaaagcat cttttgcttt gatgggtccc ataaaatcat
    11821 aatcgaagtt ttccggatta taaccattaa aactctgtgg tacatccttt aaaactgtat
    11881 tggggtgaat taaaccttga tcaagggcta atccataaat aaatggtttc agagttgaac
    11941 caggtgacct tttggtttct acaccattga tttgtccact tatcttgtta ttaaaaaaat
    12001 cagcagatcc catcaacgct ttaatactca tagtacggct atccacaagt aataccgccg
    12061 cgttgtgaac accaaacatt ttttttcttg ctaaataatg ccttgtaatt cgctcaacta
    12121 ttaactgaag cctgtaatcc aaagttgttg aaaaactttg tgataaacca ggattttcct
    12181 tcagtaccat atttacaaaa tgaggagcca ggaagggtag gttttgattg gattgcataa
    12241 ctaaaggcag tccaaacaga ggctgcttct cctggtcctg cggatgttga ttcagccatc
    12301 tggcaaataa acgatttctt atttttttta attcactgtt ttttgatgtc ctttttactg
    12361 gattttgagg gataatgctt aaagttagtg cctctggtaa accaagttta ctaacaggct
    12421 tattaaaata aattaagctc gccgccccta ctccctctat atttccacca taaggcgcca
    12481 aattgaaata agcctcaaga attttatctt tatcataatg acgctctatt tgtaacgccc
    12541 taataatttg ccataatttt ccagaaattt tcttggagtt aattccaaaa cgaattcgtg
    12601 ccacctgcat tgtgattgtt gaagccccga ttctgcgcga tttaactaca taagtttgcc
    12661 aaatggcttt caccatggaa aaaggattca ctcctttatg ccagcgaaag tactgatctt
    12721 cttgtaaaag tgtggcctcc attaactcct tgggtatttg agataatggt acataaagtc
    12781 tgtatttgtc atcgtcactt aaaatcaatc ttaataattg ttgattatca tcatataccg
    12841 ctttagaaaa attgaattct tctaataaag aaggcttggg agaaaaaaat aataataaaa
    12901 acccactgac aaatagcgaa cccagaatta tgctaagata ttttattact tttctcattg
    12961 c
//
LOCUS       DAXLDT010000087        12872 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXLDT010000087 DAXLDT010000000
VERSION     DAXLDT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.087"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            59..493
                     /locus_tag="ACF2SP_001976"
     CDS             59..493
                     /locus_tag="ACF2SP_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359756.1"
                     /translation="MKRIFLISIIGIVLNSAYAFEHIGECGAHTWHEVTSKSGSWKIT
                     LKMISQNGQFVDPSCAEIIETGLAQNNPLSYGLKFSKDADFNLAYTLTAVSEEQGFQS
                     KACVFVITANGPAQPDIQALSYHGAECTWRVIKGIGENFSVG"
     gene            complement(508..1488)
                     /locus_tag="ACF2SP_001977"
     CDS             complement(508..1488)
                     /locus_tag="ACF2SP_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFD2359757.1"
                     /translation="MHSFDSIAVEFDLKNHSMQKIAIEDLNIDYQDKDKIYWIHSNLN
                     QKNIFKKLREKLRLPEQVIQLCGEKDNRSTTIEIDEALTLQIQGLCSMKLNDNYDADF
                     SNLIVHLTPNYCFTASKKLSSVLFDMLKSCPKSLPYAKTSCFLLFLLLEGVINDYAKI
                     HLAYEELADQLDAQIRTPNKNTYSQVLELKHGVMKVKRYTIAIREILMRITSRNILVI
                     SEQCRTSLYNLSNHCHLIVNEIDSLRDMLNGLLGQIDNQLMQNMNETMKVLTAFAAIF
                     LPLSLITGIYGMNFYWMPELGWKYGYFWALGLIVLCALLLFLIFRKKKWF"
     gene            1765..2352
                     /locus_tag="ACF2SP_001978"
     CDS             1765..2352
                     /locus_tag="ACF2SP_001978"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFD2359758.1"
                     /translation="MSQDIEKMYQLIMEINKLKLVFRNTITNPQRNESTAEHSWSASM
                     ITLILMNELKREFKSVDELKIIKLVLIHDVVEIYAGDVLAFDLKARKDKEKVELEALE
                     KLSAVYPSFGIELDSLWHEFEERKSLEAKIAKAADAICPVFQRLQAKQSYIPFDISMA
                     QLEKTKYPYFQFSKTFSNLFEKLKADLLKEQLIFL"
     gene            2487..3986
                     /locus_tag="ACF2SP_001979"
     CDS             2487..3986
                     /locus_tag="ACF2SP_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HFD2359759.1"
                     /translation="MYDDLWKPYASTELPSLKLGGIVLNGQLLSETPIHLVLKAFNRH
                     GLIAGATGTGKTKTMQVFAEQLSLNGVPSLVMDIKGDISGLAMPGDASTKLLDRGQQL
                     HIDFTPRGFPVELLTLTPQDPGVPLRARIDDFGPLLFSLMLDLNDTQAGVVTVLFEYA
                     KANQLNLVNLDDFKSLLQFAQTDRGNQLIAAQYGGVASTSIGTIVRKIIELESQGGKQ
                     FFGEPSFVIQDLLRTDANGYGVISILRLLNMQDKPKLFSSFMLKLLSDVYRQMPELGD
                     PDKPRLVLFIDEAHLIFNYSSKALLNLIETIVKLIRSKGIGLIFCTQTPNDIPEIVLS
                     QLGLKIQHSLRAFTAKDRKALKLVSQNFPVSSYYNTEQLLTTLSIGEALVSALDAKGQ
                     PTPLVHAMIRAPESRMGPLSNEELKVLLDQSFLFGKYSQMIDAPSAKEKLAELISEQQ
                     VAKQQTSQKQNNSSVLNSLSKNTLFRQVVRQVFREFTRAILSLFKSKRN"
     gene            4067..5470
                     /locus_tag="ACF2SP_001980"
     CDS             4067..5470
                     /locus_tag="ACF2SP_001980"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFD2359760.1"
                     /translation="MINITGTYTDQYQLAMAQVCFLKGQHERIATFDYFFRALPFKGG
                     YAIFAGLEDLLDVLEQLRFEQQDIEFLQHQNMHPDFIRYLQQFKFNGTVYTSLEGDVV
                     FPTRPVVIIEANLIEAQIIETLLLNIINFQTLIATKARRIRQVAGERILIDFGLRRAQ
                     GAGGYFASRAAIVGGFDSTSNVRAGRDYSIPVSGTMAHSFIQSYDSEIQAFSDYAEAW
                     PENCILLVDTYNTLQSGVPNAIKIAKEMEKRGHRLKGIRLDSGDLAWLAKRARHMLDE
                     VGLNYVAITASNQLDEYVIKNLLEQDAPIDAFGVGTNLAIGAPDAALDGVYKLAFING
                     QPRIKLSDTPAKITIPYQKQVYRVFERDGTYRGADVVCLKTEKKIDMMHHPFEILKSL
                     SIKHYKKEALLHKVMENGKRLLPKKSLGEIARFSQQCFDRLPLEYKRFINPHIYKVGL
                     STELKMKRDQLIAEHSR"
     gene            5475..6095
                     /gene="pncA"
                     /locus_tag="ACF2SP_001981"
     CDS             5475..6095
                     /gene="pncA"
                     /locus_tag="ACF2SP_001981"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFD2359761.1"
                     /translation="MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVAS
                     QDWHPSNHISFASNHPGKKSFEKIKLGNLEQTLWPDHCVQGSIGAQFHAQLNTNPIEA
                     IFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLHEKGARELYFCGLCADICVYFTIKDAL
                     SQGFDCYLIEDATQALVKDDFIKIKKELIKQGTHIINSSNLLNNLQ"
     gene            complement(6178..6561)
                     /locus_tag="ACF2SP_001982"
     CDS             complement(6178..6561)
                     /locus_tag="ACF2SP_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2359762.1"
                     /translation="MSIQLNHTIVWVKDRDKSAHFLTEILGLPEAIPYGPFMVVEMSN
                     YVSLDFCKTDKEITPQHYAFLVTETEFDDIFKRICERKMDYWADPGQTRLGQMNYNDE
                     GRGLYFNDPNGHLLEIITKPYGSGS"
     gene            complement(6561..7775)
                     /locus_tag="ACF2SP_001983"
     CDS             complement(6561..7775)
                     /locus_tag="ACF2SP_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359763.1"
                     /translation="MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFKITEHAA
                     QQTITSFLVGYALGQLVYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLV
                     LGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
                     YGWESCFYAGTVYGAILLAMVRQLPETQKSLQLEAFQMKHLLQGYVSQFKNKPLISGG
                     ALMGGASCFVYIFAATAPFIAINLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYS
                     QRAGIQLGILISAFAALAMMMAALTHHSALIKLFFPMIFIYLGLSFIFANASTFAMSY
                     TSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCILMMWLFFSFIKSN
                     EIISSIDMNKES"
     gene            7893..8762
                     /locus_tag="ACF2SP_001984"
     CDS             7893..8762
                     /locus_tag="ACF2SP_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2359764.1"
                     /translation="MSKFDQITYFIAVVEEHGFAAAARKFGVSTAAVSRQITRLEAEL
                     KAELLVRTTRKLSLTEIGARYYQQCKKTLLELNEAEIAIASSRNEAVGILNVTSSRYF
                     AMRFLLPYLPEFMELNPKLQIKIELAERFPDLAQENVDVLFGVSMEGPPELVRKRVST
                     TRYVLCASPDYLAENGIPKTPSELGKHRYITHSMRNPDNLVTFKNNEQIYVKPVLWLN
                     DSRALCECALLGMGIIKLHEYIVAEALQDGRLIEVLPEFQESSLSVYLYYQASRYLQP
                     KIRRFIDFFTKQQ"
     gene            9174..12524
                     /gene="sdbA"
                     /locus_tag="ACF2SP_001985"
     CDS             9174..12524
                     /gene="sdbA"
                     /locus_tag="ACF2SP_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbA"
                     /protein_id="HFD2359765.1"
                     /translation="MHKKYNYYSLEKEKKTFWQHILDILKAPFRLPGWVVSFFLARNI
                     THVALNPNNIPQQRLIHLTKTSNRPEDDIVVINFKKRPPHKWFNDTLIKIANTIAALP
                     FVTPRLRTRLHYDNENDINHVNKLLAEIDALVQGKSKQKYCKGRTFDWSKIHLKGLEF
                     LDPKMRGYVYEQLHEKYGYVSYTTKRKPNIEFFTLKTPDGSELDSVQVTGEDEEQKPM
                     GERKFIITCIARDQNFINWIKDLNYTAKNLGATAISFNYRGVDYSRGLVWTENNLVDD
                     ILAQVQRLISLGAEPKNICLDGMCIGGAVATIAAAKLHEKGMKVKLNNERSFTSLSSL
                     VFGFIVPELQTANWWSPLTYGRFLLAGVVYALLTPLIWLAGWPVDVTKAWNRIPAQDK
                     MYSVVRDKDNGLYDGVIHDHFCSIASLVDSQINSILYKLSTDQPLTEEEKQILCDDQF
                     SHHFKPSQSVLKNPKYKGPHFISRQDLVAELGHQEEYTNHDYFLDRLREKFQLDRTIR
                     PVALAEDGEKDIDGIPSQLSNHKERPLIIASSGGTGHISATHGIINDLQSKTDNVVIT
                     QHHAELYKNKPFSITSVLIRIGVWFTSLPILEDILKGVMRFIGYPVLPSSSIFWDQMS
                     KIQQSETKKENGIETGRTRPYVDMLLDIYPEGYEYTAFNNATHLTSSIEDIQTMISFK
                     GHVEEDNRNIVYQNILQRLMHAAKQNTPYTRLISTQALSLGAICDAVKYYNTVFLPVY
                     NAERGTSYQPITIDQYMTDLPSLGCIHFMNNLEELTSEQKQLMEIHAVNMSEPFKEAH
                     FGKEQGFKAVHNIDPRNNPMIRNAFKDPSLTKYLDKTQSFDLHFNVYKKEKQNALPVL
                     NGKEKITIKPHAKIASIMIGSLAANASADYVQYLLNQGYDHIFLFGGLNDSIAARIDQ
                     IINSYPDSRHDEIRKKIILLGNQSDVEMAPIMTRSNCVVIRGGGLSVMEQMAMPIMDE
                     KIVLLHHEDNEEGPLTSGLSWEDGNADKLIEYLSEKGAYAKKTSPGLCSGHLNEAEKD
                     FEKKYITQLKSKGTKEKVDLTIPQQKKPPLKKEWGRKTGYTELGHVLSHQHRFFETIP
                     EVREPFCSKEDLHHHELSSQSLVSVTVG"
     gene            12658..>12872
                     /locus_tag="ACF2SP_001986"
     CDS             12658..>12872
                     /locus_tag="ACF2SP_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ras GEF/LegG2"
                     /protein_id="HFD2359766.1"
                     /translation="MKNKSNLLPLIACFNNREINMPKILDSNAYREMENELNNLFEQI
                     YKHGKNDRTAITLQVQQFLDNLSESIR"
BASE COUNT         4076 a         2287 c         2628 g         3881 t
ORIGIN      
        1 tttcaagttg aaataattcg taaagggcaa gtgacttgat gtttttacaa ggaaaataat
       61 gaaaaggatt tttttaatat cgataattgg gatagttttg aattctgcgt acgcttttga
      121 gcatattgga gaatgtgggg ctcatacttg gcatgaagtg acatccaaat cgggttcctg
      181 gaaaatcact ttaaaaatga tcagtcagaa tggccagttt gttgatcctt cgtgtgcaga
      241 aattattgaa acaggattgg ctcagaataa ccctttgagc tatggcctta aattcagcaa
      301 agacgctgat tttaatctgg cgtatacgtt aacggctgtg agtgaagaac agggttttca
      361 atcaaaagcc tgtgtatttg ttatcactgc taatggtcca gcgcaaccgg atattcaggc
      421 gcttagctat catggggctg aatgcacatg gcgggtaatc aaagggattg gggaaaattt
      481 tagtgtcggg tagcactatg aagcggccta aaaccacttc ttttttctga aaattaaaaa
      541 caatagtaga gcacatagta cgatcaagcc aagtgcccaa aagtagccat atttccagcc
      601 taattccggc atccaataga aattcatccc ataaattccg gtaattaaac ttaaaggcaa
      661 gaaaatggct gcgaaggcag ttaatacttt catggtttca ttcatgtttt gcatcaactg
      721 attgtcgatt tgtcctaata agccatttaa catatcgcga agagagtcta tttcgttgac
      781 aataaggtgg cagtgattcg ataaattata taaagaggtt cgacattgct ctgatatgac
      841 taaaatattt cggctagtga tacgcattaa tatttctctt attgctatgg tgtaacgctt
      901 cactttcatt acgccatgct ttaattccaa aacttgcgaa tacgtgtttt tattgggggt
      961 gcgaatttgc gcgtccaatt gatcggccaa ttcttcatag gccaaatgaa tcttggcata
     1021 atcgttaatt accccttcca ggagcaaaaa taacaaaaaa caggatgttt tagcataggg
     1081 caaggatttt gggcagcttt ttaacatatc aaacaaaact gaagaaagtt ttttagaagc
     1141 agtaaagcaa taattgggtg tgagatgaac gatcaaattg ctaaaatcag catcataatt
     1201 atcattcaac ttcattgaac acaagccttg aatttgcaaa gtgagtgctt catcgatctc
     1261 tatggttgtg gatctattgt ctttttctcc acacaattga atcacttgct cgggtaatcg
     1321 taatttctct cgcaattttt taaagatatt tttttggttt aaattgctat gtatccaata
     1381 aattttatct ttatcctgat aatcgatatt taaatcctca atcgcaattt tttgcatact
     1441 atgatttttt aaatcaaatt caacggcgat tgaatcaaac gaatgcattt caatgctcat
     1501 ccttattcga ttaatttcct catatagttt aaggatagga gacttaaatg atattgacaa
     1561 acttttttta tagcctgggt tttccaatag attgtgagtg tctcgtggag agctagcctc
     1621 ttgtttgatt tttgtcttgt tttaattcta tccctatata taattcggat tctgttttgg
     1681 caaggaaaag taaatttaag ccaaaaggaa cttaaatgag tataaaatca gaatccatta
     1741 atacaggtta tttttgagtg cttaatgagc caagatattg aaaagatgta tcaactgatt
     1801 atggaaataa ataagttgaa gcttgtgttt cgcaatacca taaccaatcc tcaacgaaat
     1861 gaaagtacag cggagcattc ctggtcagcc tctatgataa cccttattct catgaatgaa
     1921 ttaaaaagag agtttaaaag cgtcgatgaa ttaaaaatta ttaaattggt tcttatccat
     1981 gatgtcgttg aaatttatgc gggagatgtt ctggcttttg atctgaaagc aaggaaggat
     2041 aaggaaaagg ttgaattgga agctcttgaa aaactaagtg ctgtctatcc ttcatttggg
     2101 atagaattgg acagtttatg gcatgaattt gaggaaagaa aaagccttga ggcaaaaatt
     2161 gctaaagcag ccgatgcgat ttgtccggtt ttccaacgtc ttcaggcaaa acaatcctac
     2221 attccttttg atatttcaat ggctcaactt gagaaaacaa aataccctta ttttcaattt
     2281 agtaaaacgt tcagtaactt gtttgaaaaa ttaaaagcgg atttactcaa agagcaactt
     2341 atttttttat agcgccattt gtatttgctt tcaacacatc ctgccagatg tcatgttaaa
     2401 gctccttcaa gacagattaa aagttggtga atcaaaacga atcctctata cttttaaaaa
     2461 ctcttattat caagaagata agcattatgt atgatgatct atggaagcct tatgcgtcta
     2521 ccgaactacc ttctttaaaa ttgggcggca ttgtcttaaa tggtcagtta ctctctgaaa
     2581 cgccgattca cttagtgtta aaggctttca atcgacatgg tttaattgcg ggagcaaccg
     2641 gaacaggtaa aacgaaaacc atgcaggtat tcgcagagca attatcccta aatggagtgc
     2701 ccagtttagt gatggatatc aagggggata tttctggcct ggctatgcca ggtgacgctt
     2761 ctactaaatt actggacaga ggtcagcagc tgcatataga ttttactccc agaggtttcc
     2821 cggtagagtt attgacactg actccacaag accctggtgt tccattacgt gcgagaattg
     2881 atgattttgg tcccctgctt ttttcgctga tgctggatct gaatgacact caggcaggag
     2941 ttgtgactgt attatttgag tatgccaagg cgaatcaact gaatttggtt aatttggatg
     3001 attttaaatc tttgctccaa tttgctcaaa cggatagagg caaccagctg attgctgccc
     3061 aatatggcgg agtcgcttcc acatccattg ggactattgt ccgtaaaatc atcgagcttg
     3121 agtctcaggg agggaaacag ttttttggtg aaccttcctt tgtcattcag gatttgctga
     3181 gaacggatgc aaatggttat ggtgtcattt ccatactaag attattaaac atgcaggaca
     3241 aacctaaact attttccagt tttatgctga agctattgtc tgatgtttat cgccaaatgc
     3301 cggaattagg tgatcctgac aaaccacgat tggttctctt tatcgatgag gcgcatttaa
     3361 tttttaatta ttccagcaaa gcattactta atttaataga aacaatagtt aagctaatcc
     3421 gatccaaagg aatcggtttg atattttgca ctcaaacgcc taacgatatc cctgagattg
     3481 ttttaagcca attggggtta aaaatccaac actcattacg tgcttttact gcaaaagatc
     3541 gaaaagcctt aaagttggta tcccagaatt ttccagtatc cagttactat aatactgagc
     3601 aactactcac tactttaagt ataggggaag ctttagtgag tgccttggat gccaagggtc
     3661 aaccgacacc tttagttcat gcgatgatta gagcacctga atcacgtatg gggcctttaa
     3721 gcaatgagga gttaaaggtt ttactggatc aatcgttcct ttttggcaaa tattctcaaa
     3781 tgatagacgc gccttctgcg aaagagaaat tggctgagct aatctctgag caacaagttg
     3841 caaaacaaca gaccagtcag aaacaaaaca attcctcggt gttgaatagt ttaagtaaaa
     3901 acaccttatt ccgacaagtg gtacgtcaag tttttaggga attcaccaga gcgattttgt
     3961 ctttgttcaa gagtaaaagg aactaggcca gttgttgccc atattaaatt tttaacttat
     4021 accgcgcatg gtcaaaataa aagcaatata atcgacaagg atatacatga taaacatcac
     4081 aggaacctac acagatcaat atcaattggc tatggctcaa gtatgttttt taaaagggca
     4141 gcatgagcgt atagcgacat tcgattattt ttttcgtgct cttcctttta aaggaggcta
     4201 tgcgattttt gctggtttgg aggatttact ggatgttctc gaacaacttc gctttgaaca
     4261 acaagatata gagtttttac aacatcagaa tatgcatcct gactttattc gctatttgca
     4321 acagtttaag ttcaatggca cggtgtatac gtcgctcgag ggagatgttg tatttcctac
     4381 gcggccagtg gttattatcg aagccaattt gattgaagca caaatcatag aaacattatt
     4441 attaaatatt attaattttc agacacttat tgcgacaaaa gccagacgca tcaggcaggt
     4501 tgcgggggaa cggattttaa ttgattttgg actaaggaga gcacagggtg ctggagggta
     4561 ttttgcaagc cgtgcagcca tagttggtgg ctttgattcg acaagcaatg tcagagcagg
     4621 gcgtgattat tcaattcctg tatcgggtac tatggctcat tcctttatcc aaagttacga
     4681 tagcgagata caagcttttt ctgattatgc tgaagcttgg ccggagaatt gcatcttgct
     4741 agtagatact tataatacgt tgcaaagtgg tgttcctaat gcaatcaaaa tagctaagga
     4801 aatggagaag agaggacata gactcaaagg cattcgcctg gatagtggcg atttggcatg
     4861 gttagccaag agggctcgcc acatgttaga cgaggtcggt ttaaactatg tggctattac
     4921 agcttccaat caactcgatg aatatgtcat caagaatttg ttagagcagg atgcgcctat
     4981 tgacgctttt ggtgtgggga ccaatctcgc gattggtgcc ccggatgcgg ctcttgatgg
     5041 ggtttataaa ttagcgttta taaatggtca acctaggatt aaactatcgg atacccctgc
     5101 aaagatcact attccttatc aaaagcaagt ctacagggtg ttcgaaagag atgggacata
     5161 tcgaggtgcg gatgttgtct gtctcaaaac ggaaaaaaaa attgatatga tgcatcatcc
     5221 ttttgaaatt ctaaaatcgc tttcgataaa gcactataaa aaagaagcat tactgcataa
     5281 agtgatggaa aacggcaaac ggttactacc taaaaaatca cttggagaaa tagccaggtt
     5341 tagccagcaa tgttttgatc gattgccttt ggaatacaag cgttttatta atcctcatat
     5401 ctataaagta ggtttaagta ccgagctgaa aatgaaaaga gatcaactga tagctgaaca
     5461 ctcaagataa ttttatgaag actttaatca tcctcgacgt tcaaaatgat ttcatgccag
     5521 gcggctcact gggagtaccg aatggtgatg cgattgttcc cgttattaac agcattttgc
     5581 attgttttga tctgattgtt gcttcacagg attggcatcc ttctaatcac atcagtttcg
     5641 cctccaatca tcctggcaag aaatcttttg aaaaaatcaa actggggaat ttagaacaaa
     5701 ctttatggcc agatcattgt gttcagggta gtattggagc gcaatttcat gcgcaattaa
     5761 atactaatcc tattgaagct atttttcgaa aaggaacaga ccccaatata gatagctaca
     5821 gtggttttta tgacaatttg cataaaaaaa ccacaggcct tgccggttat ctgcacgaaa
     5881 aaggcgctag agaactttat ttttgtggtt tgtgcgctga tatttgtgtt tattttacaa
     5941 ttaaagatgc cctcagccag ggatttgatt gctatttgat tgaggatgca actcaagcgc
     6001 ttgtgaagga tgatttcata aaaataaaaa aagaattgat aaaacaaggc actcacataa
     6061 ttaacagttc aaatttgctt aataatttac aataagaagg tctgattttt taaatttgca
     6121 gctttgagct caccaattga tgatggaact cgctgccgaa ggaaccccgc cgtattttta
     6181 agacccgcta ccataaggtt tcgtaatgat ttcaagaagg tggccattgg gatcattaaa
     6241 atataatcct cgaccctcat cattataatt catttgacct aatcgggttt gtcccggatc
     6301 agcccaataa tccattttac gttcgcaaat tcgtttaaaa atatcatcaa attcagtttc
     6361 tgttactaaa aaggcataat gttggggagt aatttctttg tctgttttac agaaatcaag
     6421 gctcacatag ttgctcattt caacgaccat aaatgggcca tatgggattg cttcaggcaa
     6481 ccccaaaatt tccgtaagga agtgagctga tttgtcccga tcttttaccc atacgatagt
     6541 atgattcaat tgtatactca tcatgattcc ttgttcatgt cgatagaaga aattatttca
     6601 ttacttttta tgaaagaaaa gaatagccac atcattaaaa tgcatagaat gatgtagatg
     6661 gctggtaata aatattgtga cattggaagt aagcttaatc ccaaaataac cactgtaacc
     6721 aatcccatat tgatgaaatt cataaccgcg gagccatggg ctttatcgga agtatagctc
     6781 atggcaaatg tcgaagcatt tgcaaaaata aaagatagtc ccaaatatat gaatatcatg
     6841 ggaaaaaata gcttgataag cgctgaatga tgggttaacg cggccatcat cattgccagt
     6901 gcggcaaacg ctgagattaa aatgcctaat tggattcccg cacgttgcga gtattttttt
     6961 gcaaattgag cagaccagat tgagccgagg gtcaggccta aggagggtaa aatattggca
     7021 gcgccatact ctgttgtact cattcctagc aaattgatgg caataaatgg tgccgttgct
     7081 gcgaaaatgt agacaaagca ggatgcgcca cccattaatg ctccaccaga gattaatggc
     7141 ttgtttttaa attgacttac atatccctgg agcagatgtt tcatttgaaa cgcttcgagt
     7201 tgaaggcttt tttgtgtttc gggaagttgc ctgaccatgg caagcagaat agcaccataa
     7261 acagtacctg cataaaagca actttcccag ccgtaatggg agtttaaaat gccgccaagt
     7321 gcaacactca aaccgggtgc tatcgcaaaa gccagcatca gataggaggt tttttgcgcg
     7381 gctaattggg gctcatagca ttcattaacc aaagtaaaag tcatcttcag tcctacaccg
     7441 gatcctaagg ctaatagaaa tcgtcctaaa acaagcaacg ggtacatgga aatcattccg
     7501 gccaatacac atagaagact gctgataatt tgcaagccaa tacctatata aagtgttttt
     7561 ttccttccat agcgattcgc tgtagggcca taaacaagtt gccctagggc ataaccaacc
     7621 aaaaaactgg taattgtctg ctgtgcggca tgttctgtga tcttgaaaaa tgcagtaatg
     7681 ctgggcagtg ccggtgtaaa taggacagca tttacggaag caaaagaaat taacaataat
     7741 aaagtcatga aagaaagttt ggtttttgga tacatgatga gagtagtttt aacgattaac
     7801 agttaagatt agtataatta ttgacaaaat aattaacaat atcataattc attaacattt
     7861 attaactata ttgcaataat aaagttgaaa tcatgagtaa atttgatcaa attacttact
     7921 ttattgctgt tgttgaagag catggttttg cagcagccgc aagaaaattt ggagtatcta
     7981 cagccgctgt tagcaggcag attacacgtc ttgaagccga gctaaaagca gagctgttgg
     8041 tgagaacaac tagaaaatta tcattaactg aaatcggggc tcgctattat cagcaatgca
     8101 aaaagactct tttggagttg aatgaggcag aaatagcgat tgctagtagt cggaatgaag
     8161 cggtgggcat attaaatgtt actagcagcc gttattttgc tatgagattt cttctccctt
     8221 atttaccaga gttcatggag ttaaatccaa aattgcagat taaaattgaa ttggcggaaa
     8281 gatttcctga tttagctcag gaaaacgttg atgtgctttt tggtgtttct atggagggcc
     8341 cccctgaact ggtgcgcaag cgagtttcta caacacggta tgtgctttgc gcatcgccgg
     8401 attatctggc agaaaatggt attcccaaaa caccttccga acttgggaag caccgttata
     8461 tcactcacag catgcgaaat ccggataatt tggttacatt taaaaataat gagcagattt
     8521 atgttaagcc tgtgttatgg ctcaatgata gtcgtgcttt gtgtgagtgc gccttacttg
     8581 ggatgggaat tatcaaatta catgaataca ttgtggctga agcattgcaa gatgggcgcc
     8641 tcattgaggt tttgcctgaa ttccaagagt ccagtttatc ggtgtacctt tattatcagg
     8701 caagccgata tttacagcct aagatacgaa gatttattga tttttttaca aaacaacaat
     8761 aaaagcaagt attttgtgtc attctggcag gtctgagttc aaagcaaaaa agttcggggc
     8821 acgtgctgtt tttagagaga ggtcaggtta tgcaacgcct cattatgtcg caaattaggc
     8881 gtatctgtta ataaatgctt caggttgctt tgttagaata ataacagtca gaaacattat
     8941 taactagaga atcttgaaat gttttttaaa cttgaaaaat ataattacat accttgcaca
     9001 gcaattttag aaacatatgg taataactct aaagttctaa ttttctgatt gtataagtgt
     9061 aataaaacat gataaaatgt gcacaatttt tactatttgt ttgcatatat attaacccta
     9121 tgggattgca atagatagtg ccactttgat ttaattaatt tttagaagtt aatatgcata
     9181 aaaaatacaa ctactacagt cttgaaaaag aaaaaaaaac attctggcaa catatactgg
     9241 atattctgaa ggccccattt aggctacctg gttgggtcgt ttcttttttc ttggcacgaa
     9301 atattactca tgtggcatta aatcctaata atattccgca acaacgttta atacatctta
     9361 ctaaaacaag caatcgtcca gaggatgata ttgttgtcat taatttcaaa aaacggcctc
     9421 ctcacaaatg gtttaatgac acgctgatta aaatagccaa taccattgcg gctcttcctt
     9481 ttgtaacacc aagattacgt acaagattac attacgataa tgagaatgac atcaatcatg
     9541 tgaacaagtt gcttgccgag atcgatgcgc ttgttcaggg caaatcaaaa caaaagtatt
     9601 gtaaaggcag gacattcgat tggtcaaaaa ttcatctgaa gggcttggag ttcctggacc
     9661 ctaaaatgag agggtatgtt tatgaacaac tgcatgaaaa atatggttat gtctcatata
     9721 caacaaaacg caaacctaat attgaatttt tcaccttaaa aactcctgat ggatctgaat
     9781 tggattcagt gcaagttaca ggtgaagacg aagaacaaaa accaatgggg gagcgcaagt
     9841 ttattattac ctgtattgcg cgggatcaaa atttcatcaa ttggataaag gatttaaact
     9901 atacagcaaa aaatctgggc gccacagcca tcagcttcaa ctacagaggc gtggattaca
     9961 gtcgcggtct tgtctggaca gaaaataatc ttgtcgacga tattctggct caggtgcaaa
    10021 ggctaattag ccttggtgct gaacctaaga acatctgcct tgatggtatg tgtataggtg
    10081 gggcggtagc aacaattgct gcagcaaaac ttcatgagaa gggcatgaaa gtcaagttga
    10141 ataatgaacg ctcttttact tctctttctt ctctagtatt tggttttatt gtacctgaat
    10201 tgcaaacagc aaattggtgg agccctttga cttatggtcg cttcctactg gccggtgtag
    10261 tttatgcttt actcacgcct ttaatttggc tggcaggatg gccagttgat gtgactaaag
    10321 cctggaatag aatacctgct caggataaaa tgtattctgt tgtgcgggat aaagacaatg
    10381 gcttgtacga tggagtaatt cacgatcact tctgttccat tgcgtcgttg gttgattctc
    10441 aaattaattc tattttatat aaattatcca ctgatcagcc gcttactgag gaagaaaaac
    10501 aaattttgtg tgatgatcag ttttctcacc attttaaacc aagtcaaagt gttttaaaaa
    10561 atccaaaata caaaggccca cattttattt cccgacaaga tttggtcgct gagcttggtc
    10621 atcaagaaga atataccaat catgattatt tccttgaccg cttgcgggaa aaatttcaat
    10681 tggatcgcac aatacggcca gtggctttgg ctgaagatgg ggaaaaggat atagatggga
    10741 ttccatcgca actatctaat cataaagagc gtcccttaat tattgccagc agtggaggaa
    10801 cagggcatat ctctgccacg catggcatca ttaatgattt acagagtaaa acagataatg
    10861 ttgtcattac acagcatcat gcggaactct acaaaaacaa gcccttctca attacatctg
    10921 ttctgattcg tattggtgtt tggtttacct ctcttcctat cctggaagac atactgaaag
    10981 gagttatgag gtttatcgga tatcccgttt taccgagctc ctccatcttt tgggatcaaa
    11041 tgtctaaaat acagcagagt gaaactaaaa aagaaaatgg aattgaaaca ggcagaaccc
    11101 gtccttatgt ggacatgctg ttggatatat acccggaggg atatgaatat acggcattca
    11161 ataacgcgac tcacttgact tccagtattg aagacattca aacgatgata agcttcaagg
    11221 ggcatgttga agaggataac aggaatatag tctatcaaaa tatattacaa cgccttatgc
    11281 atgcggcgaa acagaatact ccttacacaa gattaatttc cacacaggca ctttctttgg
    11341 gagctatttg cgatgccgtt aaatactaca atacggtatt tttgcctgta tacaatgctg
    11401 aaagaggaac ctcctatcag cctattacca ttgatcagta tatgaccgat ttgccttcac
    11461 tgggttgtat tcattttatg aataatttgg aggagctcac atcagagcaa aagcaattga
    11521 tggagataca tgcagtaaat atgagtgaac catttaaaga ggcgcatttt ggtaaagaac
    11581 aaggctttaa agcggttcat aacattgacc cacgtaataa cccaatgatt cgcaatgcct
    11641 ttaaagaccc ttcattgaca aaatacctgg ataagacaca gtcatttgat ttgcacttta
    11701 atgtttataa aaaggaaaaa caaaatgcac tgccagtact gaatggtaaa gaaaaaatta
    11761 caatcaaacc gcatgctaaa attgcttcca tcatgatagg ctctctggcg gcaaatgctt
    11821 cagctgatta tgtgcagtat ttattgaatc aggggtatga tcatattttt cttttcggcg
    11881 ggcttaatga ttccattgcc gcacgcatcg atcagatcat caattcttac cctgactcca
    11941 ggcatgatga gatacgtaag aaaattattc tcctggggaa tcaatctgat gttgaaatgg
    12001 caccgataat gaccagaagt aactgtgtgg ttatccgtgg gggtggttta agtgtcatgg
    12061 agcaaatggc tatgcccatt atggatgaga aaattgtatt actacatcat gaagataatg
    12121 aagaggggcc tttaacgtct ggtctcagtt gggaggatgg gaatgccgat aaattaatcg
    12181 agtatctatc cgaaaaaggg gcatatgcta aaaaaacatc acctggcttg tgttctggcc
    12241 atctcaatga ggcagaaaag gattttgaaa agaaatatat tacccaacta aaatcgaagg
    12301 gaaccaagga aaaagtagat ttaaccattc ctcagcaaaa gaagcctcct ctgaaaaaag
    12361 agtggggtag aaaaacaggt tatactgaac tcgggcacgt attatcccat caacataggt
    12421 tttttgaaac gattcctgaa gtaagagagc cattctgttc taaggaagat ttacatcatc
    12481 atgagctttc cagtcaaagt ttggttagtg taacagttgg ttgataaaat atgcctctaa
    12541 tcatcgaggc tcgttttagt gcggccttct ctgaaacttg tctgctcagg atagtatcaa
    12601 tttgtgattg gaatgcgtgc tgaacaatgc aacttaaaat cattttttgt ccacagtttg
    12661 aaaaataaaa gtaatttatt gccattaatt gcatgtttta ataatagaga aatcaatatg
    12721 cctaaaattt tagatagcaa tgcatatcgc gagatggaaa atgagctcaa taatcttttc
    12781 gagcaaattt ataagcatgg taaaaatgac agaaccgcga ttacattaca agttcagcaa
    12841 tttttagata atttgagcga atccattagg ga
//
LOCUS       DAXLDT010000088        12747 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXLDT010000088 DAXLDT010000000
VERSION     DAXLDT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.088"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1292
                     /locus_tag="ACF2SP_001987"
     CDS             <1..1292
                     /locus_tag="ACF2SP_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2359767.1"
                     /translation="SLQERVQNAVDVSGAFDNCFFHNFALYLLTNNLPLPEDLFHFKS
                     IINRNSKAEQLFEFFHNPESLNLFSILDKENDVSEPSGYLFEKSLILGFLLREWFPTQ
                     LVNNSEVKMEMLEGEKGVCSAFKNYKEYRSFMPKEELKTTEFGVLYEANEAFLEYFYN
                     RSENTLINKDSPFEKYFVDSSSDEEAIKNYWDAEGYTLYCQHLAKPQVKLSYIEIMTM
                     MRVINQPLTIYDRSTSSVVAECVNPKVNLPDFEVAIDALQGHYFLLKTEETEKELEEY
                     ERSHAQYKRDRSEILAHSDKPVSSLLVRATCPKGHLDEDPFVALIESLSEINSLSQID
                     TNLKNQNTDTPNCNFLLKVGASVVGASSALITLMGIAILSGQYDPGNSLAEEALATTV
                     VSSIVGLGYGLYSFFSSSPKVPELDSTNGLSQKLKMN"
     gene            1484..1903
                     /locus_tag="ACF2SP_001988"
     CDS             1484..1903
                     /locus_tag="ACF2SP_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFD2359768.1"
                     /translation="MSAKKNSIAHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFTPIVTKSDYENTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQTEVM"
     gene            1903..3813
                     /locus_tag="ACF2SP_001989"
     CDS             1903..3813
                     /locus_tag="ACF2SP_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFD2359769.1"
                     /translation="MLLIEIVLTIHCLLLLSSLFIGRAWLANKPSFKLKLLRFLLASC
                     IISPIIVHCVNSDQKPERQNYVAFDALQQYARQPIFKTESSKFNHGSSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLTEAIPYRTSGKLVIKISHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLASNREIACTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSAAGSLTSSMVKE
                     VDISHLDPRIQQIAEKQIMTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYFIAGKTFTNYSSNVESASLAEA
                     IAKSINVCLIRVSQEAGVPVIRKKLTEFGFDTSTWWQADRSDGLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETTNYSINHMLENAVTNGTGKLAVISGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVVLVVIEEGHFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSVNQ"
     gene            3863..4663
                     /gene="blaOXA"
                     /locus_tag="ACF2SP_001990"
     CDS             3863..4663
                     /gene="blaOXA"
                     /locus_tag="ACF2SP_001990"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HFD2359770.1"
                     /translation="MKKIIIFLYTGLLFCVSTWAQDTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENTTFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4723..5598)
                     /locus_tag="ACF2SP_001991"
     CDS             complement(4723..5598)
                     /locus_tag="ACF2SP_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFD2359771.1"
                     /translation="MDKSPKLMHLLIYAVHLVLVSTCCFGEPNSPEKLLKITIANKTY
                     TLSELKKQFKTIRVIISHNPAYNYQKKTYDAFDMNQILEQLMGADLQKNNANQVLLAQ
                     TIDKYVSQTPLLYFTTKGHAYLAYQETPGTISDNIITKDGRWSYINQHGKKENPGPFY
                     IVWDNTSTYPVSWPYQVISMQIVNKKELTFSHFLNPLNESASVQNGHHIFNNICSTCH
                     SLFYKGAQGRAPDLGMVMSYLTPSDISKLVKNGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNKQASKLKCEINNQ"
     gene            5965..7425
                     /locus_tag="ACF2SP_001992"
     CDS             5965..7425
                     /locus_tag="ACF2SP_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFD2359772.1"
                     /translation="MNHKPIQFKDLSLIYPHKTCFQDFSGEIHFADRIALIGRNGSGK
                     STLLKMLCGLFLAPAGEIKIPHDVRFGHLPQVLEDCPDLSGGQRLNRVLSKILSDEPN
                     VLLLDEPTNHLDSRNRRSLMRMLEHYPGTLIIASHDTELINTVTDTLWHIDLGQVTVF
                     RGIYFNYQQILEDKKASIEQELSRIARQKKESHLALMKEQERNKRARVQGEKKIAQRK
                     WPTVRSHTKLGSAITTGNKRLSHINYKKQQLLEELSSLNFPEVIKPKFRLKGLGHHKP
                     LIRIQDASVAYEAVVILEDIHFHLSGCERVALHGNNASGKSTFVKAILGESQIKRTGE
                     WAVPDCNTIGYIDQHYQHLNFNETVLDLMRSSMSHASHTEIRAHLNDFLFRKNEEVEI
                     KVKNLSGGEKARLSMALIAANPPKLLILDEITNNADLETRTHIIQVLREFQGAMLVIS
                     HDYDFLESIHIETRYQIHQGKIHHFNEKQLEVVHHD"
     gene            7418..9199
                     /locus_tag="ACF2SP_001993"
     CDS             7418..9199
                     /locus_tag="ACF2SP_001993"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2359773.1"
                     /translation="MTNYLPNHLGSFMWHFLKPYRSIVILFILFALLAGFWGPFNSLL
                     IKYFINTLAEKTSQDLSSLYWIAGLLVLNFIVFDNITWRTLGYLNYKYEAVIKNQIIS
                     QTFEYVLGGSTQFFQDNLSGRIADQITTLADNLEIILHRVSVDFLRGASLLVVSFITA
                     YFVNVLFFYILFLWFVAFASFSIWMSARLVQLSDDHASSESQLSGQLVDSLANQSNIR
                     IFSRKIYEVKRMNTFFRLVQQAFQRKELFIVLLCCAQGGMIAVMMGLASITLIYLYGK
                     GLVSIGDFALILGLSMELGHMMWYTMYQVDQFNQALGKARQSLNALVIPHGIKDKSNA
                     SQLVVTQGRIDFSKVKFHYQGGYSLFQNKSVTIESGQKVGLVGYSGSGKSTFVNLILR
                     LYEVVDGQILIDGQNLAEVTQESLRQAIAMIPQDPTLFHRSLMDNIRYGRTDATDEEV
                     ILASRKAHAHEFISLLPEGYKTLVGERGVKLSGGQRQRIAIARAILKNAPILMLDEAT
                     SQLDSITESNIQESLWDLMQGKTTLVVAHRLSTLLHMDRILVFDKGHIIEDGTHTELL
                     NHGGLYKTLWDAQVGGFLPDQQSEKGE"
     gene            9203..10159
                     /locus_tag="ACF2SP_001994"
     CDS             9203..10159
                     /locus_tag="ACF2SP_001994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359774.1"
                     /translation="MSVKQERYLIKVVSYDSNWPVQFEQEAERIKKALGSNCIEIHHI
                     GSTSVPDLVAKPVIDMIPVVLDISKVDGANAAMRALGYEAKGEYGIPFRRYFQKGDNR
                     RTHHAHIFELGNSEIDRHLKFRDWMRVHPKDREAYALLKQELARQNSDDITAYCLGKE
                     DFIATIDKKAGFNGLRMVKALTTREWDKVRHFRQFYFFDKAGLSDPYTWTFEHNAHVH
                     FVLFQGSEITGYAHLQLWSHNRAALRIIVIDELKRNHQYGTRFLALCEKWLKSQGYQS
                     LHVESSPDALKFYRNNGYINMPFDDPDGYESDPRDTAVGKIL"
     gene            10156..10683
                     /locus_tag="ACF2SP_001995"
     CDS             10156..10683
                     /locus_tag="ACF2SP_001995"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359775.1"
                     /translation="MIQIEIVIKTLELSDIPVLVDAFQKANWQKPASLFEAYYQEQRK
                     SLRVVWLAYSRDQIAGYVTLKWISQYRPFAHQKIPEIMDLNVLPSFRKQGVGTALLQA
                     AEEKAAGENDVVGIGVGLYGGHDGGYGQAQRLYVNRGYIPDGLGVTYGYKPTVPGQTY
                     PLDDDFILWFTKKLR"
     gene            10749..11498
                     /locus_tag="ACF2SP_001996"
     CDS             10749..11498
                     /locus_tag="ACF2SP_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD2359776.1"
                     /translation="MAYTVKQLAKISNVSVRTLHWYDEIGLLKPAYQGANGYRYYEEK
                     QCLKLQQILFFRELGFPLNDIQKLLMQNDFDHIKALRAHKQVLESDINRKNQLIVTID
                     KTIQHLRGKRKMSNEELYYGFDSLRQKQYEKELVKEGVVSQDWMNNYKKKISQWTQAD
                     KDRFIQEGKDINQALISAINNQLKPASPEVQVIIHRHHAWVGWNPNKEGYIGLSQRYL
                     TPEFRRFYEQQHPDLLEFIIEGMLFYANKNL"
     gene            11706..12653
                     /locus_tag="ACF2SP_001997"
     CDS             11706..12653
                     /locus_tag="ACF2SP_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD2359777.1"
                     /translation="MTQWFVKDLSQLTAVSVQTLHHYDRIGLLKPSLRLANGYRVYSE
                     KDLLKLQQIIALKFFGFELSQIKTLLAEEGNALNHFNSQARVLEQKASTLLEGAKTLR
                     RIIDSVDKNQSIPWETIIQLIEVYKMTEHLEHGWVKEIFTPDELKQYAEFEKEMKANA
                     TPEQKAAMENNWSQLVDEVKNNLKQDPDSKIGIYLGKKIMDWVNGVYGKKYAHLRTKK
                     FEQGFGEGKGLDEVGLTPEIVSWMDKAMDAYWRDRIYGILDQVGKAPSASVLALWNEM
                     LVDMYGEESSRKNAIYEIALNDDQVSPDAKAWLRNILDS"
BASE COUNT         4011 a         2202 c         2592 g         3942 t
ORIGIN      
        1 tatcgcttca agaaagagta caaaatgcag ttgatgtttc aggagctttt gataactgct
       61 ttttccataa ttttgcactt tacctgttaa cgaataattt accgcttcct gaggatttat
      121 ttcactttaa atcaatcatt aatagaaata gtaaggcaga acaattattt gagttttttc
      181 ataacccaga atcattgaat ttattttcta tattagataa ggaaaatgat gtttctgaac
      241 catctggtta tctctttgaa aagagcttga ttttaggatt tctgcttagg gaatggttcc
      301 caactcagct tgttaataat tcagaagtta aaatggaaat gctggaaggt gagaaaggag
      361 tatgcagtgc ttttaaaaat tataaagagt atcgctcatt tatgcccaag gaagaattaa
      421 aaacaacaga atttggtgtg ttatatgaag cgaacgaggc ttttttagag tatttttata
      481 atcgctctga aaatacttta ataaataagg attcaccttt tgaaaaatat tttgttgact
      541 cgtcctcaga tgaagaggca attaaaaatt attgggatgc agaaggctat acattatatt
      601 gtcaacattt ggctaaacct caggttaaat tatcttatat tgaaattatg accatgatga
      661 gggtcattaa tcagccactt acaatatatg atcgttctac ctcctcagtt gtagccgagt
      721 gtgtcaaccc aaaggtcaat ttacctgatt ttgaagtggc aattgatgct cttcaagggc
      781 attattttct tttaaaaact gaggagacgg aaaaagagtt agaagaatat gaaagaagtc
      841 atgcccagta taaaagagat agaagtgaaa tccttgcaca ctccgataag cctgtttctt
      901 ccttattggt tcgagctact tgccccaaag gtcatttgga tgaagatcct tttgttgcat
      961 taattgaaag cctatccgaa ataaacagcc ttagccaaat tgatactaat ttgaaaaacc
     1021 agaatacaga tacccccaat tgcaacttcc ttttaaaggt aggtgcttct gtagttggtg
     1081 cctcttctgc gctaataact ttaatgggga tagccatatt atctggccaa tatgatcccg
     1141 gaaacagtct ggctgaagag gcattagcta ctactgttgt atccagtatt gtgggtttag
     1201 gttatgggct ttatagcttc tttagctcta gccctaaggt accagagctt gattctacca
     1261 atggattatc gcaaaaactt aaaatgaact aacctcctga attagaaaat attgtctgta
     1321 agccgatcgt gttgatatta ggtatcaaca cgattgcgcc attcaagtaa actgttctcg
     1381 cgtgtagaga agtatttttc ccatgactat tttaacccta ctatttttat agtagaatcc
     1441 tactataaaa atagtagggc gtatgtggtt attggagaga aacatgtcgg caaaaaaaaa
     1501 ttcgatagct catgatcgac ttcttacaga agtagagctt gaattgatga atattatctg
     1561 gagccttgat aaggtaacta ttaaggaggt tgtttcacat ctgcctaagg aaaggccttt
     1621 ggcttataca accgtggcaa cagtactaaa agtgctggaa caaaagggat ttcttgagtg
     1681 tcaaaaaaat tcctatgctc atgtttttac acccatagtt acaaagtcag attatgaaaa
     1741 cacttgcatt gatcatatgg ttgccaatgt ttttgatgga gagccccgag cacttgtcca
     1801 aagacttttg ttagccagaa agttacagca tgatgacatt caagccattg aagaagcact
     1861 taaacaatta aacgctggtg aaaaacagac agaggtgatg taatgttatt aatagaaatc
     1921 gtattgacta ttcattgcct gcttcttctc agttcactat ttatagggcg cgcctggcta
     1981 gcaaataaac catcttttaa attgaaatta ctccgttttt tattggccag ttgcattatt
     2041 tcaccaataa tagttcattg tgtaaactct gatcaaaagc ctgaacgaca aaactatgta
     2101 gcatttgatg ctttgcaaca gtatgcaagg caacccatat ttaagacaga atcctcaaaa
     2161 tttaatcatg gatcgtcttc tgcctttaca tcaatgaatg tgaactactg tcaactctta
     2221 agtgtgatgt tttttttaat cgtcttgttt cgtagttatc acttgttatc aggtttaggt
     2281 cgcttgaaat tgatgttgac tgaagcaatc ccatatcgaa cttccggaaa attagttatt
     2341 aagatttctc accactgcca tgttcctttc tcaatattgc tatttaataa agcctacatc
     2401 gtattgccag tatccatatt ttcttcttcc atgaatgtga ggattgctat tgctcatgaa
     2461 gggcaacacc atcgtaatgg agattgttca tgggcctatt ttatcgaaat catacgtatt
     2521 atcttctttg gaaacccggg tgtaactcaa tggtttcgtg ttttagcaga tttacaggaa
     2581 ttgtcatgtg atgaagtatt ggttggacat caaaaaattt cggcacatga ctatggtcac
     2641 tgtctattgc aagtggtaga ggcagtatcc caatgctctc tggcatctaa ccgggaaatt
     2701 gcatgtacgg tgggtatggc tttgaacaaa gaaaacgaag actgcaaatt tataataagg
     2761 agaatttcaa tgctaactgc ttattcaccc aatgcatcca gacctttgct attagggata
     2821 gtctttgcag gattttctat cctgtcacca atctgcgttg cctattcagc agcaggatct
     2881 ttgacgagtt ccatggtaaa agaagttgat atttctcatt tagatcccag aatacagcag
     2941 attgcagaga aacaaattat gacagccgtc aaacaatatc atgcaaaatc tggagttatt
     3001 gcaatagctg atccccaaac aggcaatatc attgcatttg ctgaatcaag taaaaataag
     3061 gggttagaga gttggaaatc acgtattttt tcacccggat ctactataaa accttttatt
     3121 gcagcagctg caatcaactc tgggaatagc tctgaaacaa aaaattatga ttgtcattca
     3181 ccttatttca tagcaggcaa aacatttaca aattatagtt ccaatgttga atcagcttcc
     3241 ttagcagaag ctatcgcaaa atctatcaat gtatgcctta tcagagtttc tcaagaggct
     3301 ggagtgccgg taatccgtaa aaagctcact gaatttggtt ttgacacaag cacttggtgg
     3361 caagcggatc gaagtgatgg tttacaactg gcaatggctg cacttgggga gaatatccct
     3421 gtcacaatcg aatcgctaat aaaatcttat gccattcttg ctaacaaagg acactcattt
     3481 gacagaggaa atagtgccat tatttcagaa acaacgaatt actcaataaa tcatatgctt
     3541 gaaaatgctg ttacaaatgg cactggaaaa ctcgcagtta tttctggtgt ttctgtagct
     3601 ggtaagacag gaactgtaat tgaaaataat gataaatatc ttgctttgtt tgccgggtat
     3661 gtgccagctg ataatccgcg ttatgttgta ttagtcgtga ttgaagaggg tcatttcagt
     3721 aaaaatggaa agacattagt ttcgggagga gaattggccg caccggtatt ccgaaatgtc
     3781 gcaatggatg ctctgagcag tgtaaatcaa taactagctt atgggtttgc atatccatat
     3841 attcaagaat gttgaggttg ttatgaagaa aataattata tttttgtaca caggattatt
     3901 attttgcgtc tctacttggg ctcaagacac ctgtttttta gcaaaagaaa atcaaactgt
     3961 tttaaaacgt gagggcaatg attgtgatca acgctattcg ccagcatcaa cttttaaaat
     4021 tgcccttagc ctcatgggct ttgattctgg aatattaaag gatgagttgc atcctgaatg
     4081 gccatacaaa aaagaatatg aactttatct gaatgtatgg aagtaccctc aaaacccgca
     4141 tacctggatc agagactcct gtgtttggta ttcacaggca ttaactcgcc aattgggcat
     4201 gaaacggttt aaagggtatg tcgacgcatt tcactacggt aatcaggatg tttcgggaga
     4261 caaaggtcaa aataacggtt taacacatgc ctggctttca agctcacttt ctatctcacc
     4321 cacagagcaa attcaatttc tacaaaaaat aatatataag aaactaccgg tgagtcaaaa
     4381 agcttatacc atgactaaaa acatcatgta catccaggag ttgcctggtg ggtggaagct
     4441 ttatggaaaa acaggaactg gtcgacagtt aacaaaagat aaaagtcaaa aattgccttt
     4501 acaacacgga tggttcgtag gttggattga aaaggatgag cgtgtgatta cctttgcaaa
     4561 acatattgca gacagtaaag agaataccac ttttgccagt tttagagcga aaaatgatac
     4621 attgattcaa ttgtttaacc tgattaatga actggagaaa taatctaaaa gatggccggt
     4681 tcagtgtttt ctgatccggc tatctaactc tctgaattat aatcactggt tgttaatttc
     4741 gcattttagt ttggatgctt gtttattaac ccagattaaa aatttaatga gatcattaat
     4801 ttcttccgtt gagaggtttt tgcctatcgg tggcatgtat cctcgcccat tctttacaag
     4861 tttactgata tctgaaggcg ttaaataact catcaccata cctaagtctg gtgcgcgtcc
     4921 ttgagccccc ttgtaaaaaa gagagtggca tgtggagcat atattattaa aaatatgatg
     4981 accattttgc acagaagcag attcattcaa tggatttaaa aaatgagaaa aagtcagctc
     5041 tttcttattt actatttgca tcgaaataac ctggtatggc caactcacag ggtaggtact
     5101 tgtattatcc catacaatat agaaaggacc aggattttct tttttaccgt gttgattaat
     5161 gtaactccag cgaccatctt tggtgataat attgtcagag attgttccgg gcgtttcttg
     5221 ataagcaaga taggcatgtc cctttgtcgt aaaatacaac aagggagttt gagacacata
     5281 tttatctatt gtttgagcta ataatacctg gtttgcatta tttttttgta aatctgctcc
     5341 catcaactgc tctaaaatct gattcatatc aaacgcatcg tatgtttttt tctgataatt
     5401 atatgcagga ttatgagaga taatcacacg aatggtttta aattgttttt ttagctccga
     5461 cagggtatat gttttattgg caatagtaat tttaagtagt ttttctggtg aattgggttc
     5521 tccaaaacaa catgttgaaa ctaataccag gtgaaccgca taaatcagaa gatgcattaa
     5581 ttttgggctc ttgtccataa gtccttcttg tttgttgaaa acaaatcaga tttttcccat
     5641 actgttacta gctattatgg tttataacag gaccgatttt ccagcgcgca gagttattta
     5701 acttaaatct gagacactta taagacgcca atggaactat tatgaaaagg aagagataag
     5761 ccaggaaaaa taattttgaa tataatattg acaccttcaa ctattttaat ttatattttc
     5821 tgtaattttt ttaccagtta aaaggtaggt ttaggccgtc atgaaaatta catcttaagt
     5881 acgcccaact atagggcaaa gtgaacaaag tcctttgtga cttttagttt ttactgtcct
     5941 ggcagacatg cttaagagta aatcatgaac cataaaccga ttcaatttaa agacctcagt
     6001 cttatctatc cgcataaaac ctgttttcaa gactttagtg gtgaaatcca ttttgctgat
     6061 cgcattgcac tgattggccg taatggctca ggaaaatcaa cgctactcaa gatgctttgt
     6121 ggtctttttt tagcccctgc tggcgaaatt aaaatacccc atgatgttcg ctttggtcat
     6181 ttacctcaag tgcttgagga ttgtcctgat ttaagtggcg gtcagcgatt aaaccgagta
     6241 ttaagcaaaa tactgtcgga tgagcctaat gttttgttgt tagatgagcc cacaaatcat
     6301 ctggacagcc gtaatcgtcg ttctttaatg cgcatgcttg agcattatcc tgggacattg
     6361 atcatagcct ctcatgatac agaactcatc aatacagtca cagatactct atggcatatt
     6421 gatttaggtc aagtgaccgt attcagagga atttatttta attatcaaca aattcttgaa
     6481 gacaagaaag catcgattga acaggagtta tcaaggattg cacgacagaa aaaagaatca
     6541 catcttgctt tgatgaaaga gcaggagcgt aataaacgtg cgcgtgtcca gggtgaaaag
     6601 aagatagccc aacgcaaatg gccaacggtt cgatctcata caaagctcgg ttcagcaatt
     6661 acaacaggca ataaacgatt aagtcacatt aactataaaa aacaacaatt actcgaggag
     6721 ctttcctctc taaactttcc ggaggtaatt aagcccaaat ttaggttaaa gggacttggg
     6781 caccataaac ccttaatcag gattcaagac gcttctgttg cctatgaggc tgttgtgatt
     6841 cttgaagata ttcattttca ccttagcggt tgcgaacgag tggcattaca tggcaacaat
     6901 gcttcaggta agtcgacctt tgtcaaagcg atcttggggg aaagtcaaat taagagaaca
     6961 ggggaatggg ctgtgcctga ttgcaataca ataggctata tcgatcaaca ttatcaacat
     7021 ttgaacttca atgaaacagt cctggattta atgcgctcca gtatgtccca tgcatctcat
     7081 acagaaattc gtgctcattt aaatgatttt ttatttcgca aaaatgaaga agtagaaatt
     7141 aaggtcaaaa atctttctgg tggtgaaaag gcaaggttat cgatggcatt gattgctgca
     7201 aatccaccta aactacttat tttagatgag atcacgaaca atgcagattt ggaaacacgc
     7261 actcatatca ttcaggtgct gcgtgaattt caaggtgcaa tgctggttat ttctcatgac
     7321 tatgactttt tagaatccat tcacattgaa accaggtatc agattcatca agggaagata
     7381 catcatttca atgagaaaca attagaggta gtgcatcatg actaattatt tgcccaatca
     7441 cttgggctct tttatgtggc attttttaaa accatatcga agcatagtga tattatttat
     7501 cctatttgct ctattggccg ggttttgggg gccatttaat agcttattga ttaaatattt
     7561 cattaatact ttggctgaaa aaacaagtca ggatctatct tctttgtatt ggattgctgg
     7621 attattggta ttgaacttta ttgtttttga caatataacc tggcgaacac ttgggtacct
     7681 gaactataaa tatgaagcgg tcattaaaaa ccaaatcatt agccaaacat ttgagtatgt
     7741 gttaggagga agcacgcaat tttttcagga taatctatcc gggcgaattg ccgatcaaat
     7801 cacgaccctt gctgataatc ttgagattat tttgcatcgt gtgtctgtag attttcttcg
     7861 tggtgcttca ttattagtgg tttcatttat tacagcctat tttgttaatg tcttgttctt
     7921 ttatattttg tttctctggt tcgtcgcctt tgcctcattt agtatttgga tgtcagcgcg
     7981 attagttcag ttgtctgatg atcacgcaag ttctgaatca cagctttccg ggcagttggt
     8041 agatagcctg gcgaatcaat ccaacattcg tatcttttca agaaaaatct acgaagtgaa
     8101 acgtatgaac actttctttc gcttggtaca acaggctttt caaagaaaag agctttttat
     8161 tgttttgtta tgttgtgcac aaggaggaat gattgcagtc atgatgggtt tggcatcgat
     8221 tactttaatc tatttgtatg gtaaaggtct tgtgagcatt ggtgattttg ccttgattct
     8281 cggactgtcc atggaattgg gtcatatgat gtggtacacc atgtaccaag tggatcaatt
     8341 taaccaagcc ttaggcaaag caagacaaag tttgaatgca ttggtgatac ctcatggcat
     8401 taaagataaa agtaacgcat ctcaattagt agttacccaa ggacgaattg acttttccaa
     8461 ggtaaaattt cattaccaag gcggttattc tctgtttcaa aataagtctg taacgattga
     8521 atcaggtcaa aaagtgggtt tagtaggcta ttcaggcagt ggtaaatcga cttttgtgaa
     8581 tctgatttta cgactttatg aggttgtgga tggccagatt cttattgatg gtcaaaacct
     8641 tgccgaagtc acgcaagaaa gtttaaggca agctattgct atgattcctc aagacccaac
     8701 tctttttcat cgaagcttaa tggacaacat acgctatggc agaacggatg caacagatga
     8761 ggaagtgata ttagcctcca ggaaagctca tgcacatgaa ttcataagtc ttttgccaga
     8821 aggctataaa accctggtag gggagcgtgg ggttaaactt tcaggtggtc agcgtcagcg
     8881 cattgcgatt gcccgagcca tcttaaaaaa tgctccgatt ttaatgctgg atgaagccac
     8941 gtctcagctt gattccatta ccgaatccaa tattcaagag agtctctggg atttaatgca
     9001 gggaaaaaca accctggtgg ttgctcatcg cttgtctacc cttctacata tggaccgaat
     9061 tttagtcttc gataaaggcc atattattga agacggtacg catactgaac tattgaatca
     9121 tggtggtttg tacaaaactt tgtgggatgc ccaagttggt ggttttttgc cagatcaaca
     9181 gagtgaaaaa ggagaataag caatgtcagt caagcaagag cggtatctca ttaaagtggt
     9241 ttcttatgat tcgaattggc ctgtgcaatt tgaacaggaa gcagagcgga ttaaaaaagc
     9301 attaggcagt aattgcattg aaattcatca tattggttcc acttcagtac ctgatttggt
     9361 tgcaaaacca gtcattgata tgattccagt tgttttagat attagcaaag tagatggagc
     9421 caatgctgcc atgcgagcac ttggctatga agcaaaagga gagtacggta ttccctttcg
     9481 ccgttatttc caaaaggggg ataatcgacg aacgcatcat gctcatattt ttgaacttgg
     9541 aaactctgaa atagatcggc atttaaagtt tagggattgg atgagagtgc acccgaaaga
     9601 tagagaagct tatgcacttt taaaacaaga gttggcgcgc caaaactctg atgacattac
     9661 cgcctattgc ctgggtaaag aagattttat tgcaacaatt gataaaaaag ccgggtttaa
     9721 tggattaaga atggttaagg cactaacaac gcgtgaatgg gataaggtgc gtcatttcag
     9781 acaattttat ttttttgaca aagcagggct ttctgatcct tatacctgga cttttgagca
     9841 taatgcgcat gttcattttg ttttatttca aggaagtgaa atcacaggtt atgctcatct
     9901 gcaattatgg tctcataaca gagcggcact acggattatc gttatcgatg aacttaaaag
     9961 aaaccatcaa tatggtaccc gttttttggc attatgtgaa aaatggctca aaagtcaggg
    10021 ctatcagagt ttgcatgtcg agtcgtcacc agatgctctg aaattttatc gaaataatgg
    10081 ttatatcaat atgccttttg atgatcccga tggctatgaa agtgatccac gggatactgc
    10141 agtaggaaaa atattatgat tcaaatagaa attgtaatta aaacactgga gttatctgac
    10201 atccctgttc tggttgatgc atttcagaaa gcaaattggc aaaaacccgc ttcattgttt
    10261 gaagcctatt accaagaaca gcgaaagtct cttcgggtag tatggttggc ttattctcga
    10321 gaccaaattg ctggttacgt tactctaaaa tggatatcgc aataccggcc gttcgcccat
    10381 caaaaaattc cagaaattat ggatttaaat gtattgccct cttttcgtaa acagggagtg
    10441 gggactgctt tactgcaagc cgctgaagaa aaagcagctg gtgaaaatga tgttgtgggg
    10501 attggtgttg gtttatatgg tggccatgat ggaggatatg gacaagctca acggctttat
    10561 gtcaatcggg gttatatccc tgatggttta ggtgtaactt atggttataa gccaactgtc
    10621 ccagggcaga cctatccttt agatgatgat ttcattttat ggtttacaaa aaaattaaga
    10681 tgaaatgctt gacccttacg ttacgtgagc acttatcttt aaatggaatc atttgagagg
    10741 acagtttaat ggcttatacg gtaaaacaat tagcaaaaat atcgaatgta agtgtgcgca
    10801 ctttacattg gtatgatgaa ataggcttat taaaaccagc ctatcaaggt gctaatggct
    10861 atcgttatta tgaagaaaag cagtgcttaa aattacagca aattttattc ttcagagagc
    10921 tgggattccc cttgaatgat attcaaaaac tgcttatgca aaatgatttt gaccatatca
    10981 aagcattgag agcgcataaa caggttttgg aaagcgatat caatcgaaaa aatcaattga
    11041 ttgtaacgat tgataaaacc attcaacatt taagaggtaa gcgtaagatg tctaatgaag
    11101 aattatatta tggttttgat tcactgcgac aaaaacaata cgaaaaggaa ttagttaaag
    11161 aaggcgtggt cagccaggat tggatgaaca actacaagaa aaaaattagt caatggactc
    11221 aagctgataa agaccgattt attcaggagg gtaaagacat caatcaagca ctgatttcgg
    11281 ctattaataa tcaacttaag cctgcttctc cagaagtaca agtcattatc cataggcatc
    11341 atgcttgggt aggctggaac ccaaacaagg aagggtatat tgggctaagc caaagatatt
    11401 tgactcctga gtttagaagg ttttatgagc agcagcatcc tgatctgctc gagttcatta
    11461 ttgaagggat gctgttttat gcaaacaaga acctttgatt aaactgatag aagggctgtg
    11521 tcttatcagc cctaaattaa attggattga gaatattatt caaaaacagg acctcttgtg
    11581 aagaattctt gcttaacaat tgatgactta gtgagttgga aaaataatta ttcaagaagt
    11641 tttgaaaaac tgcttgaccc tattgttgct atatacctta cctttgtatt gaagggggaa
    11701 acacaatgac acaatggttc gtaaaagatt taagtcaatt aacggctgta tcggtacaaa
    11761 cgttacatca ctatgaccgt ataggattgc taaagccttc attacgactt gccaatgggt
    11821 accgtgttta ctctgagaag gatttattga aattacaaca gattattgct ttgaagtttt
    11881 tcggttttga gttgtcacaa atcaaaacct tactcgctga agaaggtaat gcgctcaatc
    11941 attttaacag ccaagctcgg gtgttagaac aaaaagcatc cactttactt gagggagcta
    12001 aaactttgag acgcatcatt gactctgttg acaaaaatca atctatccca tgggaaacca
    12061 ttattcaact tatagaggta tataaaatga cagaacatct tgagcatggc tgggtaaagg
    12121 aaatattcac gccggatgaa ttaaaacagt acgctgagtt tgaaaaagaa atgaaggcca
    12181 atgccacgcc tgagcaaaaa gcagctatgg aaaacaattg gtctcagcta gttgatgagg
    12241 taaaaaataa tctgaagcaa gatccagatt caaaaatagg aatttattta ggaaagaaga
    12301 ttatggattg ggttaatggg gtctacggta aaaaatatgc ccatttaagg accaaaaaat
    12361 ttgaacaggg ttttggggaa ggaaaaggtc ttgatgaggt tggattaaca cctgaaatcg
    12421 tatcctggat ggataaagcg atggatgcat actggcgaga cagaatctat gggattcttg
    12481 atcaagtagg aaaagctcct tcagcaagcg tattagctct ttggaatgaa atgctggttg
    12541 atatgtatgg tgaggaatcg tcacgaaaaa atgccatata tgaaatcgct ttaaatgacg
    12601 atcaggttag tcctgatgca aaagcctggc ttagaaatat tttagattcc taaaaatatc
    12661 aataacactg ttggatcggt attgttttac cgatccagtc agctcaattt aaaaatactg
    12721 attattatag gcaacgtccc tgaagtt
//
LOCUS       DAXLDT010000089        12629 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXLDT010000089 DAXLDT010000000
VERSION     DAXLDT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.089"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..3016)
                     /locus_tag="ACF2SP_001998"
     CDS             complement(<1..3016)
                     /locus_tag="ACF2SP_001998"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFD2359778.1"
                     /translation="MAIKNLTNNRGISEILANIPQGDVLVLNIDDILVRNPVMEYGDT
                     PTVLTDNNLPVVLRYLKETKHPVILLSTCDEKYNERTIAQLKNLDLSYDVLIHSKDKA
                     GTLIECLKKISRSKRLHVFENDKSQLEAIDKAITEAQLNIKTTYYHYKPGTQLLTDSD
                     KPKDFPENLSAIELGASLGGGTSSTYEVRDTSNLKAYVLKFGSSEVQIKVEALMNVLY
                     QRLGVPVAHVQAYRSVNQVLAKSLNFEYGERTVQLAERLKPDQIQDEQIIIAQARRNF
                     IVHAFIGNIDVAKLDNFIQTEGGEVRLIDSGACFIFRALGDLREEMSAEVSEIDSMRN
                     HEINLSGALWFGDLSPEEIKRQVHDLINQRSTIEKTIWEVSQQLEIPSFVQRQLLSGF
                     SNRLDNLAQRYGFATQRFAKRDKAAIAGKTAAGVMHIQRDKSGELFVLLSKRARHNWC
                     DNFGGKSDIEDVSLAKTAQREAKEESNGVLDYSDRELAEAPFHDIITQAANGEQYLYR
                     MYFIEGINPVDTTQLLDKEHESFHWVPLRNLQSALKTNSAVVEEEQITTQVKDINNED
                     VIIFPPLYAMLRQGPVNTLIDDLLLNSNFRTQSTQGLAEEPDSSEQSKKNPYRPVASP
                     EQVETEIITTSIKKAKVLDEIKKKGHASSKNPKTLIKEDYQSENTGERILSQSELHLK
                     AVMGDDFRVQESTAENIKRFLDKHNKGLSKEEKQRLINQAAEMIQYEKDNPERVFFYH
                     GTNSDIAYAYAVYTAIYQILATNSGIGALRMDNPLFHKCLTIEEFISHFKNISESGKV
                     SNYDSGYMESAISANLFLFGSHNVEGSNTIEYYTNNESATKTNLQNMLSASIQSMGVG
                     VGLINQLIALAKEFPYQGQGALYQVSLPLELVDNYAYAAAPGGRLNPLLTESGETTNI
                     SQLLNEMTRGRVDKQYIESLQARLMTPPDAPITMRDYHWGKPSNQEMQDNFNHKIQKL
                     AENIVFDIMKHQNQLNKLNSKLPLMRNLRDVYG"
     gene            3643..5415
                     /gene="lem20"
                     /locus_tag="ACF2SP_001999"
     CDS             3643..5415
                     /gene="lem20"
                     /locus_tag="ACF2SP_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem20"
                     /protein_id="HFD2359779.1"
                     /translation="MKSKEERFNEWRQEKAKSEQAYIKTVPLVEEYIKSFDTLAELRS
                     DVANPDSEKIKKMDAHKRRTLGVLLYAKLKLDEVIEKISDSDSQEKDLLVELNNTLSK
                     AYDNPKSDAKKRVKILIETTEHLLDIEHHSYPQLAKELCADLYALLCIAKRSLKKNEI
                     LASWFIDVHEINKKLEELLAKTEGKVESLDKILSEKLNESLETSQKDRTIQDYFNERF
                     TEILSGDEEQRAKLDKLKVSLKEVADGLSGIVELRKEKATISEKIKKIEALLKIVEEN
                     DKKVAGRQYFLDLLASYKDHFNTLMSTEGGELKKQLAGKIEQLKTPDFYQTVSSTLLW
                     GASWATSLGTVIYRQITPQAVQDRVSAYAPATLDSEAKALLQELARQQLIELKKQQSL
                     KRDELVQRYSQLSSGSSELEKLIINATSDHLEQIAAATTETSKVLDGYDQIEIKLKDK
                     LEKLKGNQFTRKQLFDFIEKNDTWIVKLSNWLAENIHEIFKSDTARMIDKARKAQQKL
                     GEFERQYQTEIESEKAKIENNDSLSSEMKVFLKNQFEETKKAGGMEQKPQKNLSKETL
                     QKLFVSLLPEHEEISSGVIFSPFN"
     gene            5436..7031
                     /gene="legA2"
                     /locus_tag="ACF2SP_002000"
     CDS             5436..7031
                     /gene="legA2"
                     /locus_tag="ACF2SP_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegA2"
                     /protein_id="HFD2359780.1"
                     /translation="MSQSVDQSQVIDRLNRYLKWHNMPVSMNEEGICNGLVTVYAKYV
                     LEGKEKRFFELLDQIVRMEADSAIESEVNQFVYDVVLTLFPHQFNKKLSQANSMCALT
                     INNKPLVSSFDFAIATQDKNWEEIFKNLALQEDEIARVSGTAHAVAVRRKNNKYIVYD
                     PNYSSGTKEFSNEHELISELHYNVFGYHNGFYIKGGLLGMMISVIRHPDNKKPRAFPQ
                     VTQLYDRYLTENNINDVAVSFHGGTFNTLEHAAMVSHAEVIKNLLEKGAKDNDHQAAG
                     NAVMYNNVEALKALLGKNRETELFQRLFHAALESGREEVYDALFSMKGALPFNSSVQA
                     IRAAAKGGNSVLLAKVMNYFKRNHTGFSDLEDAIPSAIASGSVQCVKLLVNQLNESKK
                     PLDDKKRMEYLLDSIKQNQSYMVAYFLKDIPPEHLKTIYMSVSAVERTNLHILRQLQN
                     YGVPFSDMSKAVFEQKEHHFVGLRRILGVFLCKFTDFLKELLFNDCGIHCHRAEESGV
                     KKSFFAFPKKLVGVEKLQIPKLY"
     gene            7111..8034
                     /locus_tag="ACF2SP_002001"
     CDS             7111..8034
                     /locus_tag="ACF2SP_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFD2359781.1"
                     /translation="MNILLAGGSGLIGQELVRGMGEEHHFTVLGRDKDAIQKIFTTKI
                     SSLSWDELNTQDASRFDAVINLCGVNIAISRWTPKIKEEIISSRVQTTENLLHWILQQ
                     NAKPRFYCANAVGIYGLQENGDATDLTEDTAINWEHPKDYLSEVGIRWQQALQKAEEA
                     GLPVTSTRFGVVLKKGKGFLGKLYPSFICGLGSTIGDGRQYLSWIDSEDLVNAYRFLL
                     NHSEITGPVNVTSPNPCMQREFAKAFAKSLHRPLLLWTPAFIIHLLFGEMGDCLINHG
                     QKVKPKRLLEAGFEFKWTDIEKALKHQYSAI"
     gene            complement(8131..8547)
                     /locus_tag="ACF2SP_002002"
     CDS             complement(8131..8547)
                     /locus_tag="ACF2SP_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HFD2359782.1"
                     /translation="MMFQSKLATFFGALILAGSAFASQQGACPDINVIKAEGLPMAEE
                     IGMNYYLAYNISDYSTSSVWGFFIAPIEADSEEGALEASNEVLSMMNAPGVPHQQDED
                     IICSYDTGTPNLIAAAIKDGSQISPMKLKQYFKSAR"
     gene            complement(8824..10098)
                     /locus_tag="ACF2SP_002003"
     CDS             complement(8824..10098)
                     /locus_tag="ACF2SP_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine aminohydrolase"
                     /protein_id="HFD2359783.1"
                     /translation="MAKDKGFFKKAKTGKNKSSATPYTEECAIQIPSEIDNEQMHRMP
                     AGGEEDQYSRIKHMSALIKKYGNLPIITTQETSLPDYWLDLFAAIDEGDIPKAHTLFH
                     LLPQDDIILRALRAVHSEDYLYQLIKYCIQAKHFGFKQLNADLVVTPKTFEILIKDCA
                     ATLFNPAKTHFSFGLPSHHAYTQMGSGFCLINKTAMLIKQAELSSVQPPRFVIIGTDV
                     NRDNGLCDILRHSFSHLSICHIDVFDSRVYPQQDFSYINNEFNSEGVDVGKNIHVWHH
                     NDLSYYAVDLSLTSRKSVGVHPALLFALEQFKASIREAKVKGQKIALYLPTGWDSHED
                     ETAYCGKFVKGRMLGKTAAHQFRFNDGDLSYFYESIFTLYNENKDCIDTIYWGLEGGY
                     DRTMYERELTLLLQVIEKQLLPQDSNSHNMSC"
     gene            10298..10702
                     /locus_tag="ACF2SP_002004"
     CDS             10298..10702
                     /locus_tag="ACF2SP_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFD2359784.1"
                     /translation="MVQHDIIQLLPKPYGAHLITNEVMQKITLRGTGLLHLFIQHTSA
                     ALALNENASPDVLIDLNHFFTQLVPEHQHYRHDIEGPDDMPAHIKSILCGSSLTIPFQ
                     ENKLLLGVWQGIYLMEFRKQAGARTIVASQLI"
     gene            10838..11899
                     /locus_tag="ACF2SP_002005"
     CDS             10838..11899
                     /locus_tag="ACF2SP_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HFD2359785.1"
                     /translation="MTNDHHVSKKKMFAVVFCVFVPNEVLLANDTAVGGEGSLPIPVS
                     QPHIKMVSELIRINGKNLNEPQMNGSWHYNCNFTFKNTLNKEVIISMAFPFPINDGNS
                     EIALPAGQQTNVGQALVYDFLVTVNDKQVPAQRGNIAPDQNKGLYYEDAYFWKTTFPP
                     LATVNIHHDYSTGATYDVMGYHWVRYVLKTGALWQDSSIGHTRLEVIPNTPTRLCSEI
                     DPKADYLNPTPSGMSISGSRADRKYIWDLRHFQPQADLSLCLFTGISYVRYKVIYPWL
                     NSDDALGKLARLSNKELRFLRNTIYAQYGRQFQSPDLQEYFSKKWWYVPNHDYSDRML
                     NEEDKKLLSMINQANKLNR"
     gene            complement(12048..12575)
                     /locus_tag="ACF2SP_002006"
     CDS             complement(12048..12575)
                     /locus_tag="ACF2SP_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFD2359786.1"
                     /translation="MAIFKNLVLTLVSSALLVTPCFSAGLTLESPAFASNAAIPQKFT
                     CNNLNYSPPLIWHDPNLGTQSYVLIVHDPDAPTGNWIHWVLFNIPAQVKQLTEGTTTP
                     AGATSGLNSWNTTGYSGPCPPAGTHRYYFTLYALDTYLNLGPEATAQDVVNAMKGDII
                     DTAELVGHYSKNASQ"
BASE COUNT         3957 a         2234 c         2472 g         3966 t
ORIGIN      
        1 gtccgtaaac atcacgcaga ttacgcatca aaggcaattt tgagtttaat ttattcaatt
       61 ggttttgatg cttcataatg tcaaatacaa tattctcagc cagtttttga attttatgat
      121 taaagttatc ttgcatttcc tgattgctgg gtttacccca atgataatct cgcatggtaa
      181 taggcgcatc aggtggtgtc atcaaacgtg cttgaagact ttctatatat tgtttatcaa
      241 ctcgacccct agtcatttca tttaataact gcgaaatgtt agttgtttca ccgctttctg
      301 ttaatagagg atttaaacgt cctcccggag ctgctgcata ggcgtagtta tctactaact
      361 ctagaggtaa gctcacttga tataaagctc cttggccttg gtatggaaat tcttttgcca
      421 aggctattag ttgatttatt aagcccacac ccactcccat tgattgaata cttgcagaca
      481 gcatgttctg taaattagtt ttggtagccg attcattgtt agtgtaatat tcaattgtgt
      541 tggagccctc cacattatga cttccaaata aaaaaagatt agcggatatc gcagattcca
      601 tataaccact atcataatta ctaacctttc cactttcaga aatgttttta aagtgactga
      661 tgaattcttc aatagtaagg catttatgaa aaagaggatt atccatacgt aaagcaccga
      721 taccgctgtt tgtagcaaga atttgataaa ttgctgtgta gacagcgtaa gcgtaagcaa
      781 tatcgctatt cgtgccatga tagaagaaga ctctctcggg attgtctttt tcatactgaa
      841 tcatctcagc ggcttgatta attagccgct gtttttcttc ttttgatagt cccttgttat
      901 gtttatctaa aaaccttttt atattctctg cagttgattc ttgaacacga aaatcatctc
      961 ccataactgc tttaagatgt aattcgcttt gagaaagaat gcgttctccc gtattttctg
     1021 attgatagtc ttctttgatg agtgttttag gatttttcga agaagcatgc cctttctttt
     1081 tgatctcatc gagcaccttt gcttttttga tagaggttgt tattatctct gtctcaactt
     1141 gttcaggaga tgcaactggc cgataaggat ttttcttgct ttgctcagaa ctatctggtt
     1201 cctcagccaa gccttgcgtg ctctgtgtac gaaaattact atttaataat aggtcatcga
     1261 tcaaggtatt gaccggtcct tgacgaagca tagcatagag aggcggaaaa ataatcacat
     1321 cctcgttgtt tatgtcttta acctgagtgg ttatctgttc ttcttcaacg acagcactat
     1381 ttgtttttaa tgcactttgt aaattcctta agggaaccca atgaaatgac tcgtgctctt
     1441 tatcaagcag ttgtgtagta tcaaccggat taatgccttc aataaaatac attcgataca
     1501 agtattgctc accatttgcg gcttgagtga taatgtcgtg aaatggggcc tcagctagct
     1561 ctctatcact ataatctagc accccattgg actcttcctt tgcctctctt tgagcagttt
     1621 ttgcaaggga tacatcctcg atatcgctct tgccgccaaa attatcacac caattatgac
     1681 gtgcacgttt tgataacagc acaaataatt caccactttt atccctttgg atatgcatga
     1741 ctcctgctgc agtttttcca gctatagccg ctttgtcacg tttggcaaaa cgctgtgttg
     1801 caaatccata gcgttgagcc aagttatcaa ggcgattgga aaaaccagaa agtaattgac
     1861 gttgaacaaa agagggaatt tcaagctgtt ggcttacctc ccatatggtt ttctcaattg
     1921 tagaacgctg atttatcaag tcatgaacct gtcttttaat ttcttcggga cttaaatcgc
     1981 caaaccatag agccccagaa agattaattt catgattacg cattgagtct atttcactaa
     2041 cttctgctga catctcttcg cgcaaatcac caagggctct aaatataaaa caggcacctg
     2101 agtcaattaa tcttacttca ccaccttctg tttgaataaa gttatccaac ttggcaacat
     2161 caatattccc aatgaatgca tgaactataa aatttctgcg tgcttgggca ataataattt
     2221 gctcatcctg gatttgatct ggtttaagcc gttcggcaag ttgaaccgtt ctctcaccat
     2281 attcaaaatt tagtgattta gctaagacct ggtttacaga acgatacgcc tgcacatggg
     2341 caacaggtac tcccaaacgc tgataaagca cattcatcaa cgcttcgact tttatttgca
     2401 cctcactaga gccaaacttt agaacataag cttttaggtt gctggtatct ctaacttcat
     2461 acgtgcttga ggttccacca ccaagtgatg cgccaagctc aatagctgac aaattctcag
     2521 gaaagtcttt tggtttgtct gagtctgtta ataattgtgt acccggtttg taatgataat
     2581 aagtggtttt tatatttaac tgagcctcag taattgcttt atcaattgct tcaagttgac
     2641 ttttgtcatt ctcaaaaaca tgtaaccttt tagagcgtga tattttcttt aagcattcga
     2701 tcagagtccc tgctttgtct ttggagtgaa ttaaaacatc atagcttaag tccaaatttt
     2761 ttaattgcgc aatggttctc tcgttgtatt tttcatcgca tgtgctaagc agtatcactg
     2821 gatgtttagt ttcttttaaa tacctgagta cgaccggaag attattgtca gttaatactg
     2881 ttggagtgtc accatactcc atcactgggt tacgcactag aatgtcatca atattcaata
     2941 ctaatacatc accttggggg atatttgcta aaatttcact aatgcctcgg ttatttgtta
     3001 gatttttgat tgccatgttt atacatctca agatacattt tgttggatat tatttgtaat
     3061 tatagataat ttaagtgaaa ttatatgagc tcccccatat ttttaatgga aacttaaaat
     3121 agaggcatag gctactggag ctaattttta tctaggaata attcatccaa tagcagtatt
     3181 agggcatttg ctctgtatct ataatcatag tttggcgagt aattgcatct cagtataatt
     3241 ttcaaataac tattggctta accaatcata ttaaagacta cggttatcaa cttcagttta
     3301 agtattatgg tgtgggtttt acggctgaat aaaaactaac tatatctact ttttcaccaa
     3361 tcaattgctt taataatcta ttgactgtcc ataaaaaaat caaatagctt ttgtaaattg
     3421 aaataaacaa agaaaaaatt tggatgaatt atccatgtgg ttttttgtca aaacaaaccg
     3481 tatatgaatg aattaggtga tataattttc actatgataa attctatgat actcgttact
     3541 taatatttag ataatattag atgaatataa tgtagtaaag atttagcggg tatttctcta
     3601 tttattgttt ggaagagtgc taactggcaa ttagggttaa ttatgaagag taaagaagaa
     3661 cggtttaatg aatggcgcca agaaaaggcg aaatcagagc aggcatatat taaaaccgtt
     3721 ccattagtag aggaatacat taaatccttt gatactttgg ctgagctcag aagtgatgtg
     3781 gctaatcctg attctgaaaa aattaaaaaa atggacgctc ataaaaggcg cactctgggt
     3841 gttttactct atgccaaatt aaaactggat gaagtgatag aaaaaatttc ggattctgat
     3901 agccaggaaa aggatttatt agttgagtta aataatactc tgagcaaagc gtatgataat
     3961 cccaaatcag acgccaaaaa gagagtaaaa attttaatag aaacgacaga acacttatta
     4021 gatatcgaac atcactctta tccacaattg gctaaagaat tatgcgcaga tctatatgct
     4081 ctattatgta ttgcgaagag atcacttaag aaaaatgaaa tactggcaag ctggttcatc
     4141 gatgttcatg aaatcaataa aaaactggaa gaattattag caaaaacaga aggaaaagtc
     4201 gaaagtttgg acaaaatcct gtcagaaaaa ctgaatgaat cacttgagac ttcccaaaaa
     4261 gacagaacga tacaagatta ttttaatgag cgattcaccg aaattttaag tggggacgag
     4321 gagcaaagag caaaactgga taaattaaag gtgagcctta aggaagtagc tgatggacta
     4381 tcaggtattg tggagttaag aaaagaaaag gccacgatta gtgaaaaaat taaaaaaatt
     4441 gaagctttac tgaaaatagt agaagaaaat gataaaaaag tagcaggaag gcaatatttt
     4501 ttagatttgt tagccagcta taaagaccat tttaatactc taatgagtac tgagggtggg
     4561 gaattaaaaa aacagttagc agggaaaata gaacaactca agaccccaga tttttatcaa
     4621 actgtttctt cgaccctttt atggggcgca agctgggcta cttctctggg tacagtcatt
     4681 tatcgccaga taactcctca agcagttcag gatagagtta gcgcttatgc tcctgcaaca
     4741 ctggatagtg aagcaaaagc tcttttgcag gaattggcaa gacaacagtt aattgagtta
     4801 aaaaaacaac agagtctcaa aagggatgaa ttggttcaga gatatagcca actatcaagc
     4861 ggtagctctg agttggaaaa actgataatc aacgccactt cagaccatct ggagcaaatt
     4921 gctgcagcaa caacagaaac aagcaaagtt cttgatggct atgatcaaat tgagattaaa
     4981 cttaaagaca aattagaaaa acttaagggg aatcagttta ctcgaaaaca attatttgat
     5041 tttattgaaa aaaatgacac ctggatagta aaattatcca actggcttgc agaaaatatc
     5101 cacgagatat ttaagtctga tacggctcgt atgattgata aggctagaaa agcacagcaa
     5161 aagctgggag aatttgaaag acaatatcaa acggagattg aaagtgaaaa agcaaaaatt
     5221 gaaaataatg attcattgtc ctcagagatg aaagtttttt taaaaaatca gtttgaagaa
     5281 acaaaaaaag caggtggaat ggagcagaaa ccacagaaaa atctcagcaa ggaaactctt
     5341 cagaaattat ttgttagcct gttgcctgaa catgaagaga tctcatctgg ggtaatattc
     5401 agtccgttta attaatcagg gattagagcg atgatatgag tcaatcggtt gatcaaagtc
     5461 aagtcattga tcgattaaat cggtatctca aatggcataa tatgccagtt agcatgaatg
     5521 aagaaggaat ttgcaatggc ctggttaccg tgtacgcaaa atacgtactc gaaggtaaag
     5581 aaaagcgctt ttttgaattg cttgatcaga tagtcagaat ggaagcagac tcagcaatag
     5641 agtctgaggt aaatcagttt gtctatgatg tcgttctcac tttattcccg catcaattta
     5701 ataaaaaact aagtcaggct aattccatgt gtgccttgac tataaacaat aaacccttgg
     5761 tttcttcttt tgattttgca attgcaactc aagataaaaa ttgggaggaa atctttaaaa
     5821 atcttgctct gcaagaggat gagattgctc gtgttagcgg aacagcgcat gccgtagccg
     5881 tcaggagaaa aaataataaa tacattgtat acgatcctaa ttactcatct ggtactaagg
     5941 agttctccaa tgaacacgaa ttgatttccg agttacatta caatgtgttc ggatatcata
     6001 atggatttta tattaagggt ggtcttttgg ggatgatgat tagtgtcatc cgccatcccg
     6061 acaataagaa gccaagagct tttcctcaag tgactcagct ttatgatcgt tatttaaccg
     6121 aaaacaatat taatgacgtc gctgtttcat ttcatggtgg aacatttaat actttagagc
     6181 atgccgcaat ggttagtcat gccgaagtaa taaaaaattt gcttgagaaa ggagcaaagg
     6241 ataatgatca tcaggctgct ggaaatgctg ttatgtataa caatgtagaa gcattaaaag
     6301 ctttactggg aaaaaataga gagacagaac tctttcaaag gctatttcac gcggctttgg
     6361 agagcgggag agaagaagtt tatgatgcct tgttttccat gaaaggtgcc ttgccattta
     6421 attcgtctgt tcaagccatt cgagctgcag caaaaggggg taattctgtt ctcttggcca
     6481 aggtaatgaa ttattttaaa agaaatcata ctggattttc agatcttgaa gacgccattc
     6541 cttctgcaat tgccagtgga agtgtccaat gtgtcaaatt acttgttaac caattaaatg
     6601 aaagtaaaaa acctttagat gataaaaaaa ggatggagta tttgcttgat tcgataaagc
     6661 aaaatcaatc ttatatggtg gcttattttc ttaaagatat cccaccggaa catttaaaaa
     6721 caatctatat gagtgtctct gctgttgaga gaacaaatct gcatatttta cgtcagttac
     6781 agaattatgg agttccattt tcagatatgt caaaggctgt ttttgaacaa aaagagcacc
     6841 attttgtagg tttaagacgg atactaggtg tttttctgtg taaattcact gattttctta
     6901 aagaactctt attcaatgat tgcggaatac attgccatcg agcagaagag agtggagtaa
     6961 aaaaatcatt ttttgccttc cctaaaaaac tggttggagt tgaaaaatta caaatcccca
     7021 aactttattg aggcacggta ctaatgacaa acagtacgca tcagtttata ttaattaaat
     7081 taagatggcc tgaaaggagc ttgattcatt gtgaatatct tattggcagg aggctctgga
     7141 ttaataggac aagagttagt tcgtggaatg ggagaggagc accattttac tgtgctgggg
     7201 cgtgataagg acgcaataca aaagattttt acaacaaaaa tatcctcttt gtcctgggat
     7261 gaattaaata cccaggatgc cagtcggttt gacgctgtaa ttaatttatg tggcgtcaat
     7321 attgccattt cacgatggac accaaaaata aaagaagaaa taatcagttc gagagtgcaa
     7381 acaacagaaa atttgcttca ttggattttg cagcagaatg ccaaacctcg cttttattgc
     7441 gccaatgcag ttgggattta tggtttacag gaaaatggtg acgcaactga tttgactgaa
     7501 gatacagcaa ttaattggga acacccaaag gattacctaa gtgaagtggg aattcgttgg
     7561 cagcaagcct tacaaaaggc agaggaagcg ggccttcctg tgacttccac acgctttggc
     7621 gttgttctaa aaaagggaaa gggtttttta ggaaagctct atcccagttt tatttgcgga
     7681 ttaggaagta ctattggaga tggtcgccaa tatctttcct ggatagacag tgaggatttg
     7741 gtcaatgcct atcggttttt gttaaatcat tctgaaatta ccgggcctgt gaatgtgacc
     7801 tcaccaaatc catgcatgca aagagaattt gctaaagctt ttgccaagtc tctgcatagg
     7861 ccactattat tgtggactcc tgcttttata attcatcttt tatttggcga aatgggtgat
     7921 tgtttaatca atcacgggca aaaggtaaaa cccaaacgac ttttggaagc tggttttgag
     7981 tttaaatgga ctgacattga aaaggcatta aagcaccagt attctgctat atagtaacag
     8041 catttttaag aggatttcat gatttggagg gatatttaaa ataccctgta agttactgaa
     8101 tacttgacag ggtatttaaa ggagtactaa ttaacgagca ctcttaaagt attgttttaa
     8161 tttcattgga gaaatttgag agccatcttt gatagcagcg gcgattaaat taggagtgcc
     8221 tgtgtcataa ctgcaaatga tgtcttcgtc ttgttgatga ggaactccag gagcattcat
     8281 catgcttaac acttcattcg atgcttccaa agcgccttcc tcggaatctg cttcaattgg
     8341 agcaatgaaa aagccccaca cagaagaggt gctgtaatca ctgatattat aagccagata
     8401 gtaattcatg ccaatttctt cagccatagg caaaccttcc gccttgatta cattgatatc
     8461 tgggcaagca ccttgttgag aagcaaaagc agaaccggcg agaattaaag cgccaaaaaa
     8521 agtcgctaat ttagattgaa acatcatgtt ttttactcct gagttatttt taattccgcg
     8581 caaagagtat catttgccta gaagatttac aataatgttg taatttgaga ttggagggta
     8641 aacggaaaac aaactggcct tattctcgga ggtatgatca ttgattacta atgctatcgt
     8701 ccagaaccag caatcaacac agtatggtaa atcgctaatc gtttttgaaa tcaatagtaa
     8761 ctatttcatg gccaaaacag ttcgcgctat cattactaaa gttaaaattt gaaataataa
     8821 ccattaacag gacatattat gcgaattgga atcttgagga agcaattgtt tctcaatgac
     8881 ctgcaacagc agagtaagtt ctctttcata catggtcctg tcatatccac cttccaagcc
     8941 ccaatagatg gtgtcaatgc agtctttgtt ctcgttatac aaagtgaata tggattcata
     9001 gaagtagctt agatcaccgt cattaaagcg aaattgatga gcggcagttt ttcccagcat
     9061 ccggccttta acaaatttac cgcaatacgc tgtttcatct tcatgagaat cccatccggt
     9121 cgggaggtat aaagcaattt tttgcccttt tactttcgcc tctctaatcg atgctttaaa
     9181 ttgctctaaa gcaaaaagaa gcgcggggtg cacacctact gattttctgg acgttaagct
     9241 gagatccacc gcataataac ttaaatcatt gtggtgccat acatggatgt tttttcctac
     9301 atctactccc tcagagttga attcgttatt aatgtaggaa aaatcttgtt ggggatatac
     9361 tcttgaatca aacacatcaa tgtggcaaat cgatagatgg gaaaatgagt gtcttaaaat
     9421 gtcacatagg ccattgtcgc gattgacgtc ggttcctata atgacaaatc taggtggttg
     9481 aacagaactc aactcagcct gttttatcag catagctgtt ttattgataa ggcaaaaacc
     9541 gcttcccatt tgggtatagg catgatggct tggtaaacca aaagaaaaat gggtttttgc
     9601 cggattgaac agagttgctg cacaatcctt gattaaaatt tcaaaggttt ttggggtaac
     9661 gaccagatcg gcatttagtt gtttaaagcc aaaatgtttc gcttgaatgc aatatttaat
     9721 gagttgatac agataatctt ctgaatgaac agctcgtaat gctcgtaaaa taatatcatc
     9781 ttgcggtagc agatgaaaaa gcgtatgggc ttttggtata tctccttcat ctatcgctgc
     9841 gaataagtct agccaatagt ctggtagact ggtttcctga gttgtaataa ttggtaaatt
     9901 cccatatttt tttatcagtg ctgacatgtg ttttatgcgt gagtattgat cttcttcgcc
     9961 gcctgccggc atgcggtgca tttgctcatt gtctatttca gatgggattt gaatggcaca
    10021 ttcttcagtg tatggagtgg cagaactctt gtttttcccc gtttttgctt ttttaaaaaa
    10081 tcctttatcc ttggccatat ccattcctaa atagcgtggt taatttatta tattttagaa
    10141 taggcgagtg agagatatta atccagagat ttgttaagct catgactatc aagacaagag
    10201 gcgctacttt ctttataatg caaatagagt ttatggacag aatttcattt tgttctgact
    10261 cgataattcc tccgttatcc aattaagtcg agggtctatg gtacaacatg acataatcca
    10321 actgcttcca aaaccttatg gtgctcatct aatcactaat gaagtgatgc aaaaaattac
    10381 gttgagaggt accggattat tacatttatt tattcagcat acctctgcgg cacttgcctt
    10441 aaatgaaaat gcttcccctg atgtattgat tgatctaaac catttcttta ctcaacttgt
    10501 tcccgaacat cagcattatc gtcatgatat agaggggcca gatgatatgc ccgcccatat
    10561 taaatcaatt ttgtgtggct catctttaac tattccattt caggaaaaca agttgctatt
    10621 gggtgtttgg caaggcattt atttaatgga attcagaaaa caagcaggag caagaacaat
    10681 tgttgccagt caattgattt aggatcatag ggcataactc tactcaccat tgaatcaatc
    10741 tgagcaactc ttgtatcttt ctgtgtttca ttcataagaa agaaaaacct cctgttctta
    10801 actatacttt atattatttt gcaaccagga cagcctcatg acgaatgatc atcacgtatc
    10861 taaaaaaaag atgtttgctg tggttttttg tgtttttgtg ccaaatgagg ttttattagc
    10921 taatgatact gctgtaggtg gggagggatc tttacccatt cctgtttctc agccgcatat
    10981 aaaaatggta agtgagttaa tcaggatcaa tggaaagaat ttaaatgagc ctcagatgaa
    11041 tggttcttgg cattataatt gtaatttcac ttttaaaaat acattaaata aagaagtcat
    11101 tatttcaatg gcttttccct ttcctataaa cgatgggaat tcagaaattg ccctgccagc
    11161 gggacagcag actaatgttg gtcaagcatt ggtttatgat tttctggtta cagtcaatga
    11221 taagcaagtt ccagcacagc gcggtaatat cgccccggat cagaataaag gcctttacta
    11281 tgaggatgcc tatttctgga aaaccacctt tccacccttg gcaacagtca atattcatca
    11341 tgattattcg acgggagcta cttatgatgt gatggggtat cattgggttc gttatgtttt
    11401 aaaaacaggt gctctttggc aggatagcag tatcggacat actcgacttg aagtcattcc
    11461 caatacacca acgcgtttat gctctgagat agatccaaag gcagactacc tcaatcctac
    11521 cccatcaggg atgagcattt caggctcacg agcggataga aaatacatct gggatttacg
    11581 gcattttcaa ccccaagcag atttgtcact ttgcttattt acggggatta gttatgttcg
    11641 ctataaggtg atttatcctt ggttaaattc agatgatgca ttaggcaaat tagccagatt
    11701 gagtaataaa gaattacgat tcttacgaaa tactatttat gcgcaatatg ggcgtcaatt
    11761 tcaatcaccc gatttacaag aatattttag caagaaatgg tggtatgtac caaatcatga
    11821 ttattcagac cgtatgctca atgaagaaga taagaaatta ttgtcaatga ttaatcaggc
    11881 aaataaactt aaccgctgat aaattcttta caataaatcc cgtatgaggt ttctaaatta
    11941 ataaaagctg attatgacag gtgactatta ccacccgggt ctatttttgt tttatgccaa
    12001 ttcttgtatt tgttactgga tcgcttgaga aaaacattgt tgcaatttta ttgagatgca
    12061 ttcttactat agtgtcctac taattcagcc gtatctatga tatctccttt catggcattc
    12121 actacatctt gagcagtggc ttctgggcca agattaaggt aagtgtctaa agcatagagt
    12181 gtaaaatagt aacggtgtgt acctgcaggc gggcaaggac cactgtaacc ggtggtattc
    12241 cagctgttta acccactggt tgcgccagca ggagttgttg tcccttctgt gagctgtttc
    12301 acttgagcag ggatattaaa caatacccaa tgtatccaat tacctgtagg agcatcagga
    12361 tcatgcacta ttaacacata agattgggtt cccagattgg gatcatgcca tattagtggg
    12421 ggagaataat ttaaattgtt gcatgtgaat ttttgaggaa ttgctgcatt cgatgcaaat
    12481 gccggacttt ccaatgttaa tcctgcagaa aaacacggtg taacaagtaa tgcagagctg
    12541 actaatgtca agacaagatt tttaaatatg gccatcactt tccctgtttc tattgtttac
    12601 ttacctgaag ttattgagag tctagtctt
//
LOCUS       DAXLDT010000090        12442 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXLDT010000090 DAXLDT010000000
VERSION     DAXLDT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.090"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            125..751
                     /gene="lvgA"
                     /locus_tag="ACF2SP_002007"
     CDS             125..751
                     /gene="lvgA"
                     /locus_tag="ACF2SP_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LvgA"
                     /protein_id="HFD2359787.1"
                     /translation="MADGDIEIKAGFVDTDLDDRKLTMIDDLNNPLAIVERVYLIWWH
                     WADFHLHVISPHIDTITPAIVIEPELIPGSNDHEFVYSIHDSGSKLSTSKSQDMFSAG
                     MSMCKLFYTIEKMVHILVDRLKSGGVSLEEEVQIAFAGHEIAQRKAFESIINLPYNVV
                     VTNFDPGIWGEKYLQNVKRLADKGYGYPPESPRENYKHPVSSATTARK"
     gene            complement(781..1158)
                     /locus_tag="ACF2SP_002008"
     CDS             complement(781..1158)
                     /locus_tag="ACF2SP_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFD2359788.1"
                     /translation="MFFIKYLGALIYDLIILFILFFAYTSVLLLFTHGQAIPPATRWY
                     QFSLFSITLVYYYGSIRCGGQTVGMKAWRFKLTTDSNKTLSRQQIIARFFYFIPATLI
                     APFCLKGNYVLLHQWTQTHFKKV"
     gene            complement(1308..2447)
                     /locus_tag="ACF2SP_002009"
     CDS             complement(1308..2447)
                     /locus_tag="ACF2SP_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL and HDOD domain-containing protein"
                     /protein_id="HFD2359789.1"
                     /translation="MIAKRPIYNQQLKCVAFEILSCRNVILNEELTRQFYELIYHTDT
                     QLPLFVPFSLKKLLEQFETPINNPIILKLSADEIESVYSLTELQDSLFSIALLINTPQ
                     QLAWLNFAEYIGLTEQLMSKSDVTKVVQYSKAKNRKIIAYDLNQHVNFEKCKAMTMDY
                     YCGDFLLNYIAEEHTEIAANKLNVLQLIQELQKEDCDLNTITHIIQEDPLLSYQLLKL
                     VNSVGFSSGPSIESIDQAVARLGTINLKKWVMLFSMKNISDKPIEILESGLIRAHMAE
                     ELAKKSSNIFSQSAYTAGLLSILDCLLNKPMPALVEQITLAEEIKNALTNKSGPLGNL
                     LSLVIAYEEGHWEEVTQLNINGIDLSQLYIDSLALVSQGTKIMSN"
     gene            2768..3142
                     /gene="sdhC"
                     /locus_tag="ACF2SP_002010"
     CDS             2768..3142
                     /gene="sdhC"
                     /locus_tag="ACF2SP_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFD2359790.1"
                     /translation="MNKKRPINLDLSSLKFPPMAIASILHRVSGVLIFLLFPFVLFIL
                     GCSLQSEESFAQTKIILANPYYKLLLWAFGASMIYHVIAGIRHLIMDMGFGESLRAGR
                     MSAVSIIALAVILAIFLGIWIW"
     gene            3136..3483
                     /gene="sdhD"
                     /locus_tag="ACF2SP_002011"
     CDS             3136..3483
                     /gene="sdhD"
                     /locus_tag="ACF2SP_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFD2359791.1"
                     /translation="MVNNVTSLTGNGLKDWLIQRVTAVYFAAYLIFLFGFILANPDLN
                     FSQWHLLFANKLFQIATTIGLMALTLHAWIGIWTVTTDYMKCTVIRLSVQLLVVLWLL
                     GQFIWGLMIIWGQ"
     gene            3485..5254
                     /gene="sdhA"
                     /locus_tag="ACF2SP_002012"
     CDS             3485..5254
                     /gene="sdhA"
                     /locus_tag="ACF2SP_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFD2359792.1"
                     /translation="MTVARNRFDAVIIGAGGAGMRAALQMTNSGLKVALLSKVFPTRS
                     HTVSAQGGITAALGNADEDDWRWHMYDTVKGADYIGDQDCIEYLCKTGPEAVYELEHM
                     GLPFSRMDNGKIYQRQFGGQSKHFGGEQAARTCAAADRTGHALLHTLYQQNLKAKTHV
                     FSEWYALDLVKNSQGRIAGVTAMCMETGEIVFFQTRVCILATGGAGRIFQSTTNAFIN
                     TGDGFGMALRAGIPLQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLINKDGERFMERY
                     APRVKDLASRDVVARAMALEIRAGKGFDPKGVDHVKLKLDHLGADLIMSRLPGIRELS
                     MKFAGVDPIVEPIPVVPTCHYSMGGIPTNMHGQVITKTDGKEHVVEGLYAVGECACVS
                     VHGANRLGGNSLLDLVVFGRAAGIHVEELWQSNQLPDMSYVSEDDVVASLVRYERWEN
                     SKDGESHAVIRDEMQRVMQEDFGVFRTGEVMASGLKRLQALRDRLAHAKLDDKSQIFN
                     TERVEAMELDNLMATAYATALSAIARTESRGAHSREDYPKRDDANWIKHTLYFEEGEV
                     IDYRPVNTSPKYVEPFEPKERVY"
     gene            5267..5989
                     /locus_tag="ACF2SP_002013"
     CDS             5267..5989
                     /locus_tag="ACF2SP_002013"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFD2359793.1"
                     /translation="MADSRNVILSIYRYNPEVDAKPYMKDYEIEIPAKSDPMLLTLLE
                     RLKAEQDPSITYRRSCREGVCGSDGMNINGTNGLACITHLSQLKTDKIVIRPLPGFPV
                     IRDLAVDMTQFYQQYERIEPYLQNDEVAPARERLQSPEERAQLDGLYECILCACCTSS
                     CPSYWWNPDKFVGPAGLLQARRFLADSRDKATQHRLDLLQDPFSVFRCRTIMNCTNVC
                     PKGLNPTQAIAKIRTQMLTQET"
     gene            6047..8857
                     /locus_tag="ACF2SP_002014"
     CDS             6047..8857
                     /locus_tag="ACF2SP_002014"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFD2359794.1"
                     /translation="MSSSDLQKEWASSYLSGGSMAYVDSLYEDYLADPDSVSEDWRAV
                     FSALPKVNGATKEVSHRDIRDYFLQNADKKLNKIIQIADSQQYQVASLINDFRSLGHL
                     AAKLDPLEMTERMPVPRLELAYHNLADVDVNRTFFAGTSFNGPEMTLGEIYNALRETY
                     CRSIGIEYMHISDTEVTEWLQHKMESVRGCPEFSKQEKLNILKDLIAADGLERYLGTR
                     YVGQKRFSLEGGDSLIPMMKELIRRSGVNQVKELVIGMAHRGRLNVLVNVLGKEPGLL
                     FQEFEGKIKYERTGDVKYHLGFSSDIKTESGAIVHLALAFNPSHLEIIGPVVEGSVRS
                     RLGRRNDLAKKDKVVPIVIHGDAAFAGQGVVMETFNFSQARGYCTGGTIHIVINNQIG
                     FTTSNPLDARSTLYCTDVAKMVQAPVIHVNGDDPEAVVFATQVAFDFRMKFKRDIVID
                     LVCYRRHGHNEADEPSVTQPKMYKKIKSMPTLREKYGEQLVSVNLLTKTELDKLVDSY
                     REALDKGKAVVDLVHEDYEGKQSLDWTPYINAKWTDKVDTTISKEELEKISSQLNKLP
                     DGMKLHPVVERLLAERNKMTAGEIPMNWGYAEIMAYASLLHEGYGVRISGQDSGRGTF
                     AHRHAALHDIEKGDTFIPLEHISTNPKRYFTVIDSVLSEEAVLAFEYGFASSEPSFLV
                     IWEAQFGDFANGAQVVIDQFISSGEQKWGRLCGLVMLLPHGYEGQGPEHSSARLERYM
                     QLCAQHNIQVCTPTTPAQIFHLLRRQVIRNFRKPLIVMTPKSLLRHKLAVSPLEALFK
                     GKFHTVIPEIDALDAQKVTKVVLCCGKVYYDLLQMRRDKNLNHVAIVRIEQLYPFPKK
                     ALMAELEKYQKANEVVWCQEEPQNQGVWFSSQHNIRDCLRSDQTLQYAGREFAAAPAV
                     GSPGLHAEQQVALVEQALLGKK"
     gene            8893..10122
                     /gene="odhB"
                     /locus_tag="ACF2SP_002015"
     CDS             8893..10122
                     /gene="odhB"
                     /locus_tag="ACF2SP_002015"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFD2359795.1"
                     /translation="MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKV
                     VLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPVKKEEIKIS
                     ESESVSTKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
                     TAEVQKESSMRTLSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVM
                     DMRSQYKDSFEKKHGVKLGFMSFFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIA
                     VSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEMQGGTFTITNGGVFG
                     SLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFL
                     VSVKELLEDPSRLLLNV"
     gene            10185..11348
                     /gene="sucC"
                     /locus_tag="ACF2SP_002016"
     CDS             10185..11348
                     /gene="sucC"
                     /locus_tag="ACF2SP_002016"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFD2359796.1"
                     /translation="MNLHEYQAKQLFASYGLPVPRGEVAYNVEDALLVASQLSTSRWV
                     VKAQVHAGGRGKAGGVKLVSSKDELAAVAKSMLGTRLVTYQTDARGQPVNAILVEETC
                     EIDKELYLGAVVDRATRRVVIMASTEGGVEIEKVAHETPEKIFKVVVDPLVGVMPFQC
                     RETAFKLGLKDDQIKQFTHLMMGLGKMFVDCDLSLLEINPLVITKSGQLICLDGKINI
                     DGNALFRQPKLKNMRDVSQEDDRENRASDWELNYIPLDGTIGCMVNGAGLAMATMDVI
                     KLHGGEPANFLDVGGGATKERVSEALKIIVSDEKVKGILVNIFGGIVRCDLIADGILA
                     AVKEVDVKIPVVVRLEGNNAQLGAEILNKSNLNVIAATSLTDAAKKIVAAVSE"
     gene            11423..12298
                     /gene="sucD"
                     /locus_tag="ACF2SP_002017"
     CDS             11423..12298
                     /gene="sucD"
                     /locus_tag="ACF2SP_002017"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFD2359797.1"
                     /translation="MSVLVDKHTKVLCQGFTGKQGTYHTEQALAYGTKMVGGVTPGKG
                     GQMHLGLPVFNTMREAVEETQADASVIYVPAPFCKDSIIEAAEAGVKLIVCITEGVPV
                     LDMLQVKVYIENNTQARLIGPNCPGIITPGECKIGIMPGYIHLPGKVGIVSRSGTLTY
                     EAVKQTTDKGFGQSTCIGIGGDPIPGTSFIDALSLFEKDPKTEAIIMVGEIGGSAEEE
                     AAEFIKSHVKKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTAAEKYAALEAAGVKTVR
                     SPADLGDAIAEVTGW"
BASE COUNT         3760 a         2063 c         2819 g         3800 t
ORIGIN      
        1 tatctatttt atgctataca ttgattttac ggctttttta ccttttctta aggtaggttt
       61 aatgttctat tgtcaatttt gggctaatat atcaataata tccttactat ccgggtaaat
      121 ataaatggca gacggcgata tcgaaatcaa agcaggtttc gtagatactg atctagacga
      181 tcgcaaactg acaatgatag atgatttgaa taatccatta gcgatagtgg agcgagtcta
      241 tctaatatgg tggcattggg cagattttca tcttcatgtt atttctccgc atattgatac
      301 tattactcct gcgatagtca tagaacccga actgatacct ggttctaatg atcatgaatt
      361 tgtttattcc attcatgata gtggctctaa attatctaca tccaaaagcc aggatatgtt
      421 tagtgcaggc atgtctatgt gcaaactttt ttatactatt gaaaaaatgg ttcatattct
      481 ggtagatcgc ttaaaatctg gcggtgtcag tttggaggaa gaggttcaga tagcttttgc
      541 tggccatgaa atagcccaga gaaaggcttt tgagtcaatt attaatttac cttataatgt
      601 tgttgtgact aattttgatc ccggaatatg gggtgaaaaa tatttacaaa atgtgaaacg
      661 cctggcggat aaagggtatg gttatcctcc tgaatcccca agagaaaatt ataagcatcc
      721 ggtatcttct gcaactactg cacgaaaata aataattagc gagaatggct caacagtgtt
      781 ttaaactttt ttaaaatgcg tttgggtcca ttgatgcaaa agcacataat tcccctttaa
      841 gcaaaaaggt gctattaagg tggcgggaat aaaataaaaa aatcgggcga taatttgttg
      901 tcttgatagt gttttattag aatcagttgt gagtttaaat ctccaggctt tcatacctac
      961 agtttgtcca ccacagcgaa tagaaccata ataataaaca agagtgatac tgaataatga
     1021 aaattgatac caacgggtgg caggaggtat ggcttgtcca tgggtgaata atagcaggac
     1081 agaggtgtat gcaaaaaata aaatgaataa gatgattaaa tcataaatca atgcacctaa
     1141 gtatttgatg aaaaacataa ctaatacata tcctttattg atttcaacta ttaaattttg
     1201 gaatattaat catttaggaa aacacattct aaattttttg ttgctcaaag agaagcctgg
     1261 gtcaggcata gaaaaataaa aagaatattg tttaaattgt tgcgcaatca gttactcatg
     1321 atttttgtac cctggcttac caaggctaaa ctgtcaatat ataattgact taaatcaatt
     1381 ccattgatgt taagttgagt tacttcttcc caatgccctt cttcataagc aattaccaaa
     1441 gacaatagat ttcctaatgg tcctgattta tttgtcagag cattttttat ctcctcagcc
     1501 agggtgattt gttctaccaa tgctggcatg ggtttgttta ataagcagtc taatatggat
     1561 aataatcctg cagtataggc actttggctg aaaatattcg aacttttttt agcgagctct
     1621 tccgccatat gagctctgat tagacctgac tccaatattt ctataggttt atctgagata
     1681 tttttcatgg aaaacagcat aacccatttt ttcaggttaa tggtgccaag cctggcaacg
     1741 gcttgatcga tggattctat gcttggccca ctagaaaatc caactgaatt gaccagtttt
     1801 agaagttgat agcttagtaa tggatcttct tggatgatat gagtgattgt gtttaaatcg
     1861 caatcttctt tttgtaattc ctggatgagt tgaagtacat ttaatttgtt tgctgctatt
     1921 tctgtgtgct cctctgcaat gtagttaagc aggaaatcgc cacagtaata atccatggtc
     1981 atagccttac atttttcaaa attaacgtgt tgatttagat cataagcgat gattttacga
     2041 ttttttgcct tactgtattg tactaccttg gttacatccg atttactcat taactgctca
     2101 gtcaatccta tgtattcggc aaaatttagc caagccaatt gttgaggggt attaatcagt
     2161 aaggcaatag aaaatagaga gtcttgtagc tcagttagtg aataaaccga ttcgatttca
     2221 tctgcggata attttaaaat aatggggttg ttgattggtg tctcaaattg ctcaagcaat
     2281 ttttttaggg aaaaaggaac aaataaaggt aattgggtgt ctgtgtggta aatgagctca
     2341 taaaattgtc ttgttaattc ttcattaagt attacattgc gacaggataa tatttcaaaa
     2401 gcgacacatt ttaattgttg attatatatt ggtcttttgg cgatcacaaa gatctccttt
     2461 tgctcacatt atgtaatcat agtacgggtt tttgtaattg gccaatggac tattcatttt
     2521 ccttataaac tgaactttgc gtgataaaaa cagagtgcta tacttcccat gcatgaattg
     2581 aaatttagta attctaaata gtttttaaga cccaattttt tatttttcaa agccatttgt
     2641 tgcgcagtat acttaatgaa ttatgtttct caataagtct aaagggtgaa tgtgaaataa
     2701 ttgaatggga ttcaatattt agaagaaaat atattgatga cattttctta tgaggaagaa
     2761 attaatagtg aacaagaaaa gaccgatcaa tcttgattta agcagtttga aatttccgcc
     2821 tatggctatc gcatcgatat tacatagggt ttcaggagtt cttatatttt tattatttcc
     2881 cttcgtattg tttattcttg gttgttcctt gcaatcagag gaatcttttg cacagactaa
     2941 aataatttta gctaatccct actataaact tctgttatgg gcttttggcg cctctatgat
     3001 ttatcatgtc atcgcaggta taagacatct gataatggat atgggatttg gtgagagtct
     3061 tcgtgctggt cgtatgtcgg ctgtttcaat tatagctctt gcagttattt tggcaatttt
     3121 tctaggaatc tggatatggt aaacaatgtc accagcttaa caggaaatgg gttaaaggat
     3181 tggttaattc aaagagttac tgctgtctat tttgcagcct atttaatttt tttatttgga
     3241 tttatattgg caaatcctga tttaaacttt tctcaatggc atcttttgtt tgccaataag
     3301 ctatttcaaa ttgcaacaac aattgggttg atggctttaa cattgcatgc ctggataggt
     3361 atttggacag taactacaga ttatatgaaa tgtactgtta tacgtttatc agtacagtta
     3421 ttggttgttt tatggctcct tggtcaattt atctggggcc ttatgatcat ttggggacaa
     3481 tagcatgaca gtggcacgta acagatttga tgcggtaatt ataggagctg gcggtgcagg
     3541 aatgcgagct gcactccaga tgacaaattc tggtttaaaa gtagcgttgt tatcaaaagt
     3601 atttccaacc cgctcacata ctgtgtcggc tcaaggtggt attacagccg ctttgggaaa
     3661 tgctgatgaa gacgactggc gctggcacat gtatgatacc gtgaaaggtg ctgattacat
     3721 tggtgatcag gattgtatag agtatctatg taaaaccggg cctgaagcag tttatgaact
     3781 ggagcatatg gggctacctt tttcacgaat ggataatgga aaaatctacc aacgtcagtt
     3841 tggtggccaa tcaaaacact ttggtggaga acaagcggcc cgtacctgtg ctgcggcaga
     3901 tagaacaggg catgctttgc tgcacactct ttatcaacag aatttaaaag ctaaaactca
     3961 tgtatttagt gaatggtatg ctctcgatct ggtaaaaaac tcgcaaggaa gaattgcagg
     4021 tgttactgcc atgtgtatgg agactggcga gattgtattt tttcagactc gagtttgtat
     4081 tttggcgact ggaggagccg gtagaatttt tcaatctaca acgaatgctt ttattaatac
     4141 tggcgatggc tttggaatgg cactaagagc tggtatacct cttcaagata tggaaatgtg
     4201 gcaatttcat ccaactggca tagcgggagc tggtgttctg gttacggagg gttgccgagg
     4261 tgaaggtggt tatttaataa acaaagatgg tgagcgtttt atggagcgtt atgctcccag
     4321 agtaaaggat ttggcttccc gggatgtggt cgctcgcgct atggctttgg aaattcgggc
     4381 tgggaaagga tttgatccaa aaggtgtcga tcatgttaag ttaaagcttg accatctggg
     4441 cgctgatttg attatgtctc gcttgcctgg cattcgcgag ttatcaatga aatttgcggg
     4501 tgtggatcca atcgttgagc cgattccagt tgttccaact tgccactaca gtatgggagg
     4561 aatacccacc aacatgcatg gccaggttat aactaaaact gatggcaaag agcatgtggt
     4621 ggaggggttg tatgctgtag gggaatgtgc ttgcgtttca gtgcatggtg ccaatcgatt
     4681 aggtggtaat tccttattgg atttggttgt gtttggccgt gcagccggga tacatgttga
     4741 agaattatgg caatcaaatc aattgcctga tatgtcttat gtaagtgaag acgatgtagt
     4801 agcttctctg gttcgttatg agcgttggga aaactcaaag gatggtgaaa gccatgcggt
     4861 aatacgagat gaaatgcaaa gagtaatgca ggaagatttt ggagtattcc gtaccggtga
     4921 ggtaatggct tcaggactga aacgtttaca agccctgcgc gatcgattag cacatgctaa
     4981 acttgatgat aaaagtcaaa tttttaatac agagcgcgta gaagctatgg aattggataa
     5041 tttaatggca actgcttatg caacggcttt atctgcaatt gcgcgaactg aaagcagagg
     5101 agcgcacagc agggaagatt atccaaaacg agatgatgca aactggatta agcacacatt
     5161 gtattttgag gaaggtgagg tgatagatta tcgtccggtt aatacctcac ctaagtatgt
     5221 cgagcctttt gagccgaaag aacgcgttta ctaatgagga tattttatgg ctgatagtag
     5281 aaatgtaatc ttgtcgattt atcgctataa tcctgaggtg gatgccaagc cttacatgaa
     5341 agattatgag atagaaatcc cggctaaaag tgatcccatg ttacttacct tgcttgagcg
     5401 cttgaaagct gagcaagacc cttctattac ttacagacgg tcatgccgag aaggagtatg
     5461 tggctctgac ggtatgaata tcaatgggac taatgggtta gcttgcatta cacatctttc
     5521 acaattaaaa acagataaaa ttgttattcg tcctttgcca ggttttccag taattcgtga
     5581 cttggcagtt gatatgacac aattttatca gcagtatgaa cgcattgagc cttatttaca
     5641 gaatgatgaa gtagcacctg ctcgagagag gttacaatct cctgaagagc gggcacaatt
     5701 ggatggcttg tatgagtgca ttttatgcgc ttgctgtacc agttcatgtc cttcttattg
     5761 gtggaatcct gataaatttg taggccctgc tggactttta caagctaggc gttttttagc
     5821 agacagtcgt gacaaggcaa cacaacatcg tttggattta ttacaagatc ctttcagtgt
     5881 atttcgttgt cgtactatta tgaattgcac gaatgtatgc ccgaagggac tcaatccaac
     5941 gcaagcgatt gcaaaaattc gcacgcaaat gttgacgcag gaaacgtaat aaatcagtcc
     6001 ttgttactca gaaagtttct gagtgaaccc tttggagaga atgataatga gcagttctga
     6061 tctgcaaaaa gaatgggctt cttcctattt gtctggagga agtatggctt atgttgatag
     6121 tctttatgag gattacctcg cagaccctga ttcagtgtca gaggattgga gagcggtatt
     6181 tagcgcttta cccaaagtaa atggtgctac caaagaagta tctcatagag acattcgcga
     6241 ttattttttg cagaatgctg acaagaagct taataaaata attcaaattg cagatagtca
     6301 acagtaccag gttgccagtt tgattaatga ttttagatca ttagggcatc ttgccgcaaa
     6361 actggatcca ttggaaatga cggaacgcat gccagtaccg cggctggagc ttgcttatca
     6421 taatcttgcc gatgttgatg ttaatcgcac tttttttgct ggtaccagct ttaatgggcc
     6481 ggagatgact ttgggggaaa tatacaacgc ccttcgcgaa acatactgtc gaagtatagg
     6541 cattgagtat atgcatatat ccgatactga agtcactgaa tggttacaac ataagatgga
     6601 atcagttcgt ggctgtcctg aatttagtaa gcaggaaaaa ttgaatatcc ttaaagattt
     6661 gatagcagca gacggtttgg agcgttatct aggaacccga tatgttggtc aaaaacgatt
     6721 ttcgcttgag gggggagatt ctttaattcc aatgatgaaa gaactcatta gaagatctgg
     6781 tgtaaatcag gttaaagagc ttgtgattgg aatggcacat agagggcgct taaatgtgtt
     6841 agtgaatgtt ctcggtaaag aaccaggact attatttcaa gaatttgaag gaaaaattaa
     6901 atacgaacga actggcgatg tgaaatatca tctgggtttt tcctctgaca taaaaacaga
     6961 atcaggtgct attgtgcacc tggctttggc gtttaaccct tcacacttgg aaattattgg
     7021 gccagtagtt gagggatccg tacgttcacg tttgggaaga cgcaacgatt tggctaaaaa
     7081 agataaagta gttcccattg tcattcatgg tgatgctgct tttgcaggtc agggcgtggt
     7141 tatggaaacc tttaacttct cccaggcacg tggttattgt actggtggaa cgatccatat
     7201 tgttatcaat aatcagatag gatttacaac gagcaatcct ttggatgccc gttcaactct
     7261 ctattgtaca gacgttgcca aaatggttca ggcgccagtt attcatgtca atggagatga
     7321 tcctgaggca gtagttttcg ctactcaagt cgcttttgat ttccgcatga agtttaaacg
     7381 agacatagta attgaccttg tctgttacag acgtcatggc cacaacgaag ctgatgaacc
     7441 ttcagtgacc cagccaaaga tgtataaaaa aatcaaatcc atgcctacat tacgtgaaaa
     7501 atatggagaa caattggtca gtgttaattt attaactaaa acagaattag acaaattggt
     7561 cgattcctac agagaagcac tagataaagg caaggcggtt gtcgatttgg ttcatgaaga
     7621 ttatgaagga aagcaatctt tagattggac tccttatata aatgccaagt ggacggataa
     7681 agtagacaca accattagca aggaagagtt ggaaaaaatt tcatcccaat taaacaaatt
     7741 gcctgatgga atgaaactgc atcccgttgt ggagcgtttg ttagctgaac gaaataaaat
     7801 gaccgctggt gaaatcccta tgaattgggg ttatgctgag atcatggctt atgccagctt
     7861 attgcatgaa ggctatggcg taagaatttc tggacaagat agtggtcgag gaacttttgc
     7921 ccatagacat gctgcactcc atgatattga aaaaggagat acttttattc ctttggaaca
     7981 tatatcaacc aatcccaagc gctattttac cgtgatagat tcagttcttt ccgaagaagc
     8041 ggtattagct ttcgaatatg gctttgcttc gtctgagcca tctttcctgg ttatttggga
     8101 agctcaattt ggtgattttg caaatggagc acaagtcgtt atcgatcaat ttataagttc
     8161 cggtgagcaa aagtgggggc ggctgtgcgg attggtgatg ctattacccc atggctatga
     8221 aggacaaggc cctgagcact cttctgcgcg attggagcgt tatatgcagc tttgtgcaca
     8281 gcacaatatt caagtatgca ctccaacaac tccagcacaa atatttcatt tgttgagaag
     8341 acaagttatt cgtaatttca ggaagccttt aattgtcatg actcccaaaa gtcttttacg
     8401 acataaattg gctgtttcac ccttggaagc tttgtttaaa ggaaagtttc atacagtaat
     8461 tccggaaatc gatgccttgg acgcacaaaa agtaacaaag gtggtgctat gctgtggtaa
     8521 ggtttattat gatttattac aaatgcgtcg cgataaaaac ttaaaccacg tagcgattgt
     8581 ccgtatagag caattgtatc catttcccaa aaaagcatta atggctgaat tagagaaata
     8641 ccaaaaggct aatgaagttg tctggtgtca ggaagagcca caaaaccaag gagtctggtt
     8701 ctcttctcag cataatatac gtgattgctt gcgttctgac caaaccttgc aatatgctgg
     8761 cagagagttt gcggctgcac ctgcagttgg tagtcccggg ttgcatgcag agcaacaagt
     8821 tgctttggtt gagcaagcat tacttggtaa aaaataaatc taaacaatat aatattaaga
     8881 gaaggtataa ccatgtctat tgaagtcaaa gtacctgttc tacccgagtc agtagctgat
     8941 gcaacagtgg ctgcatggca taagaaagtg ggtgataaag tatctcgcga tgagaaccta
     9001 gttgatctgg aaacagataa agttgtgctt gaagttccat cacctgtgga tggggtattg
     9061 tctgacattc tgtttaatac cggagatacg gttggttcag gagacttact ggcaaaaatt
     9121 tctcaaagtg tttctgtaga atctcaaaaa actgagaaag aagagaaacc tgtcaaaaaa
     9181 gaagaaataa aaatttccga aagtgaatca gttagcacaa aagaagataa atcaaccagt
     9241 ccggtagtaa gacggatgat ggctgaaaat gatttgaaag ccgggcagat tcaaggtacc
     9301 ggtaaagatg gcaggataac caaagaagat gttttatctt acatagaatc caatagagaa
     9361 aaatcaaacc aaactgctga agtccaaaaa gaatcttcta tgagaacact ttcttttggt
     9421 ggcagggaag aaaaacgtgt ccctatgacg agactaagag ccaagattgc ggaacgttta
     9481 ttacaagcac agcacaatgc tgccatgtta accactttta acgaagtgaa tttaaaggca
     9541 gttatggata tgcgttctca gtataaagac agctttgaaa aaaaacatgg tgtaaaactt
     9601 ggatttatgt ctttttttac gaaggcggta gtggagtcgc ttaaacgatt tccggctgta
     9661 aatgcttcaa ttgatggcca agatattgtt tatcatggct tctatgatat aggaattgct
     9721 gtttctactg aaagaggttt agtcgttcct gtgattagag acgctgatca gttaagtatg
     9781 gcagaaattg aattagccat taatgacgct gcatcaaaag ccagaaccgg aaaattatct
     9841 atggaagaga tgcaaggggg gacatttacc ataactaacg gtggagtctt cggttcacta
     9901 ttagctactc ccattatcaa cccaccgcaa actggtattt tagggatgca taaaattgag
     9961 gaaagaccta ttgtagagaa agggcaaata gtaatccgtc ctatgatgta tgttgcttta
    10021 tcctatgatc atcgtttaat tgatggaaaa gattctgtac aatttttagt gagtgttaaa
    10081 gagctgttgg aagatccatc ccgactttta ctgaatgttt aatgagtcat gtttaactgc
    10141 aagacccgtc ttgacgggtc ttgctttttt aaaaggtaat tagaatgaat ttacatgaat
    10201 atcaagccaa gcaattgttc gctagctatg gtttaccagt tcctcgtggt gaggtcgctt
    10261 ataatgttga agatgccttg ctcgttgctt ctcaattatc aacctctcga tgggttgtga
    10321 aagcacaagt ccatgctggt ggaagaggta aagcaggtgg tgtgaaactg gtttctagca
    10381 aagatgaatt ggctgcagtt gcgaaatcca tgttaggcac acgtctggtt acttatcaaa
    10441 ctgatgccag aggtcaacca gttaatgcca ttttggttga agaaacctgc gaaatagaca
    10501 aggaattata tcttggtgct gttgttgata gagccaccag acgagttgtc attatggcct
    10561 ccacagaagg tggtgttgaa attgaaaagg ttgctcatga aacacctgag aaaattttca
    10621 aggtggttgt tgacccctta gttggtgtta tgccctttca atgtcgtgaa acagctttta
    10681 aacttggatt aaaagacgat caaatcaaac aattcactca tttgatgatg ggattaggga
    10741 aaatgtttgt tgattgtgat ttaagtttat tagagataaa tccgcttgtc ataacaaaat
    10801 cagggcaact gatttgtctg gatggaaaaa ttaatatcga tggaaatgct ttattccgac
    10861 agcctaaatt gaaaaatatg cgagacgttt cccaggaaga tgatcgagaa aatcgtgcca
    10921 gtgattggga acttaattat attcctttgg atgggacaat aggttgcatg gtaaatggtg
    10981 ctggtttggc tatggctacc atggacgtga taaaattaca tggtggtgag ccggctaatt
    11041 tcctggatgt tgggggaggc gccacaaaag aaagagtcag cgaagctttg aagattattg
    11101 tgtcagatga aaaagtcaaa ggaattctag ttaatatttt tggtggaatc gttcgttgtg
    11161 accttattgc tgatggaatt cttgctgctg taaaagaagt ggatgtaaaa attcctgttg
    11221 ttgtcagact ggaaggtaat aatgcccagt taggtgctga gatattgaat aaaagcaatt
    11281 taaatgtgat tgctgcaacg agtttgacag acgcagccaa gaaaattgta gcagcagttt
    11341 ccgaataaag aataaattaa ttttggcatt attggtaagg tagttttcaa agcggatatg
    11401 ccattaaatt acgaggttaa caatgagtgt attagttgat aaacatacaa aagtactatg
    11461 tcagggtttt actggaaaac aaggtactta tcatacagag caagcgctgg cttatgggac
    11521 taagatggtg gggggagtta ctccaggtaa gggtggtcaa atgcatctgg ggttgcctgt
    11581 atttaatact atgcgtgaag cagttgagga aacccaggcg gatgcaagcg ttatctatgt
    11641 tcctgcacca ttttgcaaag attccattat tgaagcagca gaggcaggtg ttaaactgat
    11701 tgtttgtatt acagaaggtg tgcctgtatt ggatatgttg caagtaaaag tgtatataga
    11761 aaacaataca caggctcgat taattggtcc aaattgtcca ggaataatta caccaggcga
    11821 atgcaagata ggtattatgc ctggctatat ccatttaccc ggaaaagttg gaattgtttc
    11881 acgttcagga actttaacct acgaagcagt aaaacaaact actgataaag ggtttggtca
    11941 aagtacttgc ataggaatag gtggcgatcc aattccagga accagcttta ttgatgcttt
    12001 gtctttattt gaaaaagatc ctaaaacaga agctatcatc atggtaggag aaattggtgg
    12061 ttcagcagag gaagaggcag ctgaattcat taagtctcat gtaaaaaaac ctgtagtatc
    12121 atacatcgct ggagtcacag cacctgctgg aaagcgtatg gggcatgctg gagcaattat
    12181 ctctggtggg aaggggaccg cagcagaaaa atatgctgct ctggaagcgg ctggtgtgaa
    12241 aacagttcga tctcctgctg acttgggaga cgcaatagct gaggtaactg gttggtaaga
    12301 aaatactttt tcttacttaa cagcccttgc attatagaaa taggaactga ttaaaataaa
    12361 ttggtgattt tccctcgaat catgttggtg ctgtgtatca agcgcagccc aacagccatc
    12421 atgataatat tgtcacttgg tt
//
LOCUS       DAXLDT010000091        12320 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXLDT010000091 DAXLDT010000000
VERSION     DAXLDT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.091"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..380
                     /gene="dotZ"
                     /locus_tag="ACF2SP_002018"
     CDS             <1..380
                     /gene="dotZ"
                     /locus_tag="ACF2SP_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 4 secretion system coupling complex
                     assembly protein DotZ"
                     /protein_id="HFD2359798.1"
                     /translation="IRKALIKAFIHCDLVKDQSQKNKYQLIDKLNQTLAVSVGAELKE
                     SILTNLGELFQILGALNTKLDEFTDRTNHLSQQAKSFRTQFYEVILRIIELIKLLPEY
                     KIDPEQDAINREPLYFDRTIGER"
     gene            515..940
                     /gene="ndk"
                     /locus_tag="ACF2SP_002019"
     CDS             515..940
                     /gene="ndk"
                     /locus_tag="ACF2SP_002019"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFD2359799.1"
                     /translation="MAKELTLSIIKPDAVAKSVIGEIYTRFEKAGLDIVAAKMIQLSR
                     EQAESFYDIHRARPFFKDLVDFMISGPVMIQVLKGDNAVAKNREIMGATNPKEAAPGT
                     IRADFADSIDANAVHGSDSLENAAREIAFFFEPHELCNR"
     gene            961..2109
                     /locus_tag="ACF2SP_002020"
     CDS             961..2109
                     /locus_tag="ACF2SP_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFD2359800.1"
                     /translation="MDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGICD
                     FAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEAN
                     RGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDKKIT
                     NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVA
                     LAVSLHAPTDELRNELVPINKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQP
                     EHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDKLMKHGINTITRKTR
                     GDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTEKSTELTISSEEIA"
     gene            2290..3072
                     /gene="pilW"
                     /locus_tag="ACF2SP_002021"
     CDS             2290..3072
                     /gene="pilW"
                     /locus_tag="ACF2SP_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFD2359801.1"
                     /translation="MLKSIRYLLIIIICLFVGACQHTQDNEEPNTIRKVNLSKAASFN
                     VQLGLGYLKQGDRPRAKKKLLTALEQQPDSADVNAALAYYFEQTKELEQAKKFYHKAI
                     TLSQNGGAQLNNYGAFLCRQGDYKNAETYFLKAVKDQNYVHTSGAYENAGLCAMAIPD
                     HDKAILYFTKALNQDPSRKESLYELVKLQSKIAHDKEALELLQKHADLVLNDKVMLAL
                     AKDIANRTGQYTLAAEYENSFNKLEQTNNISGVNDEYNSSNG"
     gene            3047..3616
                     /locus_tag="ACF2SP_002022"
     CDS             3047..3616
                     /locus_tag="ACF2SP_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2359802.1"
                     /translation="MNTIAAMDETRNNEFTNPGYELAALRQQKGYSIEYVASKLHLRA
                     RIIELIEAGDFDLLPQPVFVKGYLRAYSKLLGVSPEPFLTVFNAQYCFEKKPERAALW
                     QSKKESHKAEHFIKWFTILFAIGVLVAVGIWWQKNKDSQSIDTPKESSSGLSISETSS
                     EIKLTDLSKMESLLTPNNTQMTPLEKNGG"
     gene            3606..4886
                     /gene="hisS"
                     /locus_tag="ACF2SP_002023"
     CDS             3606..4886
                     /gene="hisS"
                     /locus_tag="ACF2SP_002023"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFD2359803.1"
                     /translation="MVVDKIQAIRGMNDVLPDSTSVWRFIEQTFINCLVRYGYKEIRF
                     PIVENTQLFKRTIGEITDIVEKEMYTFNDLNGDSITLRPEGTAGCVRACIEHGLLHNQ
                     QQKLWYLGPMFRHERPQKGRYRQFNQFGVEALGITGTAIELELISICRRLWTDLGFSQ
                     SVQLQVNSLGEINERQKYRSILVEYLHDHFHILDEDSKRRLDKNPLRVLDSKNPDLQH
                     LIQNAPKLIDVLGDDSREHFQSFCNGLETLGIPYSINPVLVRGLDYYGQTVFEWVTDQ
                     LGSQATICAGGRYDMLVEFLGGAPTPAVGFALGLERIFLLMETLNLLNESNNKQSIFI
                     IATNEEAILKALVMAESIRNAHPSLDVITNTAGGGFKSQFKKADKSGARMALILGEDE
                     IAREYVSIKDLRTEIEQISIPMTKINEFLQNYLA"
     gene            4904..5578
                     /locus_tag="ACF2SP_002024"
     CDS             4904..5578
                     /locus_tag="ACF2SP_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFD2359804.1"
                     /translation="MSVYMTEEEQLEIIKKWWKRYGNMITIFLSIVLLIIAGYRYMNW
                     HNDKIKQQASIAYENMMIAFSNQNIKSVRSYANELIKNYNHTVYADIAHMTLAKIYVS
                     KNKLEQARNELKAVASESQMTPLRQIAKIRIARLLAADKAYTNALNELSAVEDEAYLP
                     VINELKGDIYSAKGQYQDAMNAYRLAIDEVRTNGMGNLFLEMKTNELAIKTQSMITDD
                     KKVKAA"
     gene            5603..6757
                     /gene="bamB"
                     /locus_tag="ACF2SP_002025"
     CDS             5603..6757
                     /gene="bamB"
                     /locus_tag="ACF2SP_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFD2359805.1"
                     /translation="MKIRILVLILCALTQGCTYVDDYMLGKDNTPQPKELKEIQPKVK
                     MAQSWTTPVGKAHKTNEYLNIKPAIRGDVIYTADASGLVQAVNRKDGQIKWSTALKNN
                     IVSGPTVAAGYVAVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTID
                     GKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRL
                     IWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPAS
                     VYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKQTSLKARGLTAPALVGGNLAVGDK
                     TGYLHILSTQTGELLGRSQLSGGVTVSPSVSGKNMYVLTNNGMLNQLSVS"
     gene            6762..8150
                     /gene="der"
                     /locus_tag="ACF2SP_002026"
     CDS             6762..8150
                     /gene="der"
                     /locus_tag="ACF2SP_002026"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFD2359806.1"
                     /translation="MIPVIALVGRPNVGKSTLFNRITKTQDALVADFPGLTRDRQYGH
                     AQHENKSFIIVDTGGIGVDDIEVDTLMSRQSQVALNEANVILFLVDGRSGLTGIDQQI
                     AQALRKLNKKVHLVVNKTDGMNEDIACADFQSLGITDVHAISASHGGGISSLLEEILE
                     PFTTETHEATDDKAIKIAFAGRPNVGKSTLINRILGEERVVVYDMPGTTRDSISIPFT
                     REDKQYVLIDTAGVRRKSRIDEKIEKFSVIKTLQAIKEAHVCLLLLDANEGITDQDMN
                     LLGFIIESGKALVIAVNKWDGLEEDHKEKIKSELSRRLHFANFAKIRFISALHGSGVG
                     GLFKDINEAYHSAIQSFSTPKLTRLLQDISAKHTPPCINGRRIKLRYAHLGGHNPPVI
                     VIHGNQLDALPESYKRYLNNEFIKHLGLVGTPLKIEFKGGQNPFANKKNKLSQRQVNK
                     KKRLMRWAKSKK"
     gene            complement(8227..8649)
                     /locus_tag="ACF2SP_002027"
     CDS             complement(8227..8649)
                     /locus_tag="ACF2SP_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD2359807.1"
                     /translation="MYTNILFATDLLNEHSHLTEKAANIAKQFNAKLYLLHVIELPTS
                     ILIAQGLGFTELANPSKEDAQTVLSLIGESLQIPQEQQFVEIGSVKEHILNKAKDLNC
                     QLIIIGSHSATGLPSILGSTAHAVVNHSVCDVLTLKAD"
     gene            complement(8704..9129)
                     /gene="folK"
                     /locus_tag="ACF2SP_002028"
     CDS             complement(8704..9129)
                     /gene="folK"
                     /locus_tag="ACF2SP_002028"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFD2359808.1"
                     /translation="MNVCYLSLGSNQKNPERQIRQAIKEIKQIPSTCLIKVSRLYWNK
                     AWGFENQQEFCNVVIEIRTTLLPHKLLKWCQKIENRHKRVRRKFWGPRTLDIDIILYG
                     YRLIRTKDLIVPHPCFLLRDFVLVPLRELNSNLKIPNYT"
     gene            complement(9126..10397)
                     /gene="pcnB"
                     /locus_tag="ACF2SP_002029"
     CDS             complement(9126..10397)
                     /gene="pcnB"
                     /locus_tag="ACF2SP_002029"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFD2359809.1"
                     /translation="MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRD
                     LLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSS
                     DASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTSGVEDVKQKL
                     IRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDE
                     ITKLYQCGEAESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDG
                     KPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQ
                     VIREIWLLQYRFDKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWATFQE
                     VDDVEKNNMVTQLASTTPGRPKKRRKRKIAK"
     gene            10704..11435
                     /locus_tag="ACF2SP_002030"
     CDS             10704..11435
                     /locus_tag="ACF2SP_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFD2359810.1"
                     /translation="MDFIDIGLSLTALIILEVVLGIDNLVILSILTEKLPREKRKKAR
                     RWGLTFAWMTRLLLLGSAVFLVKLVKPILTIGSLVFSARDLFLILGGAFLIWKSTDEI
                     HQDVMNEPLMEPVSKNVSSSATFRGVVIQIALLDIIFSLDSVLTAVGLTSHFMVMAVA
                     ITIAIIIMIWASEPVSKFISEHPTIKMLALSYLILIGTVLVADGFEFHVPRGYLYFAM
                     GFSLAVESLNLIKNERVKRKKLKRE"
     gene            11438..11860
                     /gene="hemJ"
                     /locus_tag="ACF2SP_002031"
     CDS             11438..11860
                     /gene="hemJ"
                     /locus_tag="ACF2SP_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HFD2359811.1"
                     /translation="MLFVKAFHIIALVAWFAGLFYLPRLYVYHAETLDKISVDRFKIM
                     ERRLYYGITWPAALATTFLGLWLFTYNLSYYLKAGWMHMKLALVLLLWVYHLTCGHFL
                     KSFALGKNTRSSRFYRFFNEMPTLLLVGIVLLVVVKPF"
     gene            complement(11857..12033)
                     /locus_tag="ACF2SP_002032"
     CDS             complement(11857..12033)
                     /locus_tag="ACF2SP_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFD2359812.1"
                     /translation="MQEYKRYICVICGFIYDEAEGWPEDGIEPGTRWEDVPENWFCPD
                     CGAGKEDFEMVEMD"
BASE COUNT         4088 a         2121 c         2365 g         3746 t
ORIGIN      
        1 caattagaaa agcgttaatc aaggctttta ttcattgtga tctggtcaag gatcaatcac
       61 aaaaaaacaa gtaccagcta attgataaat tgaatcaaac attagctgtt tctgtaggtg
      121 ctgaattaaa agagtctatc ctgaccaatc tgggtgaact tttccaaatc ttaggcgctt
      181 tgaatacaaa gcttgatgaa tttactgatc ggactaatca tctaagccaa caggctaaat
      241 cattcagaac ccaattctat gaggtaattc ttcgcatcat tgagttaatt aaactattac
      301 ccgaatataa aattgaccct gaacaagatg cgattaatcg agaaccatta tattttgata
      361 gaacgattgg agaacgctaa cgcaaaaccc tattttctct aaaatcatac tctaactatt
      421 gactgaatgg ttgaatgcat gaatttttat tagccagacc actataaatt catgtataat
      481 aatgcacttt aaattaatct cattggagtg catgatggca aaagaattaa cattatctat
      541 tattaaacct gatgctgtag caaagtctgt gataggtgaa atatataccc gctttgaaaa
      601 agcaggcttg gatattgttg ccgctaaaat gatacaactt tcacgcgaac aagccgagag
      661 cttttatgat attcatcgtg cgcgaccatt ctttaaggat ctggtggatt ttatgatttc
      721 tggtcctgta atgatccaag tattaaaagg tgacaatgct gtcgctaaaa acagagaaat
      781 tatgggcgcg acaaatccca aagaagcagc acctggaact atacgtgctg attttgctga
      841 tagtattgat gcaaatgctg tgcatggttc tgacagcctt gaaaatgctg ctcgtgaaat
      901 agcatttttc tttgaaccac atgagttgtg caatagataa aggcaggctt gggagcggta
      961 atggaccaac aaaaagtgaa tctattaaat tataattact cgcagctacg agaattattg
     1021 atagcgtggg atgaaaaacc tttcagagct caacagctat ttcaatggat tcatcaagtt
     1081 ggcatatgtg actttgctca gatgactaat ttagggaaag ttttaagaaa taaactttcc
     1141 caattagctt gtatagattt acctgaaatc gttgcatgtc aaaaatcagc tgatggcaca
     1201 cataagtggc tattaaagct ggaatgtggg aattgtattg aaacagtatt cattccggaa
     1261 gcaaatcgag ggacactttg tgtttcatca caagtaggtt gtgctttaaa ttgttctttt
     1321 tgctccacag caaagcaagg atttaacaga aatttatcaa cagctgaaat aattggccaa
     1381 gtatggctgg cagcacgcga actatcagat aataatggca ctcatgataa aaaaataact
     1441 aacgttgtga tgatgggaat gggtgaaccg ttacttaatt tcgataatgt tgtttctgct
     1501 atgaacatca tgatggatga tttagcctat ggactatcca aaagacgggt tacattaagt
     1561 acttcgggtg ttttaccgga aatggaacga ctcagagaag tcagtcctgt cgcgttagca
     1621 gtatccttac atgcaccaac agatgaacta cgtaacgagc tagtgccaat taataaaaaa
     1681 tatcctctct ctcaattaat atcactatgt aaacgatatt ttaaagacga accaagaaga
     1741 aaagttactt ttgaatacgt catgctaaaa ggggttaatg atcaacctga acatgctagt
     1801 caactaatta aacttttgca taatgtgcct gcaaaagtta atttaattcc ttttaaccca
     1861 tttcctttaa ctcaatatca gagatcatcc cgggaaacaa ttgatgcctt tcgagataaa
     1921 ttaatgaaac atggtattaa tactattact cgcaaaactc gtggagatga tattgatgca
     1981 gcatgcggcc aacttgctgg cgaggtaaaa gataaaacga gcagatcaca acgatggcaa
     2041 aaacttcatt tcatgagcaa aacagaaaaa tcaactgaat tgaccatttc ttcggaagaa
     2101 attgcataac acagtcttga tataataact gagaaccaat taagaacttt ctccagtatt
     2161 cctggttgga gattatgtat aataaaggtg ttttcacctg cagactcaca ttaaatagtg
     2221 acaaatcatt tttaatttgg acttaagtgg ttataatgcg aaatcatttc taacgtaaag
     2281 tgattaatcg tgctaaaaag cataaggtac ttattaatta taataatttg tttatttgtt
     2341 ggagcttgtc agcatacaca agataacgaa gagcctaata ccattagaaa agtaaacctt
     2401 agcaaagcgg cttcttttaa tgtgcaatta gggttgggat atttgaagca aggcgacaga
     2461 cctagagcaa aaaagaagct gctaaccgcc ttggaacagc aaccagattc agcggatgtt
     2521 aatgcagcat tagcctatta ttttgaacaa accaaagaat tggagcaagc gaaaaaattc
     2581 tatcataaag caataaccct atcgcaaaat ggtggtgcac aattgaataa ttacggtgct
     2641 tttctatgtc gacaggggga ttataaaaat gctgaaactt acttcctgaa ggccgttaaa
     2701 gatcaaaact atgtgcatac ctcaggcgct tatgaaaatg ctggattatg tgctatggct
     2761 atacctgatc atgataaagc cattttatat tttacgaagg ccttaaatca ggatccatcg
     2821 agaaaagaat cactatatga attagtaaaa ttacaaagta aaatagcaca tgacaaagaa
     2881 gccctcgaac tgcttcaaaa acatgctgat ttagtattaa atgataaggt aatgcttgca
     2941 ttagcgaaag acatagcaaa tagaacaggc caatatactc ttgcagcaga atatgaaaat
     3001 agcttcaata aactggagca aactaataat attagtggag taaacgatga atacaatagc
     3061 agcaatggat gagacaagaa ataatgaatt taccaatccc ggctacgagc tggcggctct
     3121 tcgccagcaa aaagggtatt caatcgagta tgttgcgagc aaacttcatt tacgagctcg
     3181 tattatagag ctcattgaag caggagattt tgatttattg cctcagccag tttttgtaaa
     3241 agggtacttg cgcgcctatt caaaattatt aggagtttca cctgaacctt ttttaaccgt
     3301 ttttaatgca caatattgtt ttgaaaaaaa accggaaaga gcggccctat ggcaaagtaa
     3361 aaaagagtct cataaagcag aacattttat aaaatggttc accattttat ttgcgattgg
     3421 agttttagtc gcagttggga tatggtggca aaaaaacaag gatagccagt ctatagatac
     3481 cccaaaagag agttcatctg gattatcaat cagtgaaacc agttcggaaa ttaaattgac
     3541 cgatttatca aaaatggaat cgttattaac gcccaataat acacaaatga cgcctttgga
     3601 gaaaaatggt ggttgataaa attcaagcta tccgaggaat gaatgatgta ttgcctgata
     3661 gtacctcggt atggcgcttt atagaacaga cttttataaa ttgcctcgta cgatatggat
     3721 ataaggaaat tcgttttcca attgttgaga atacacagct atttaaacgt acaattggtg
     3781 aaataacaga tatcgttgaa aaagaaatgt acacattcaa cgatttgaat ggtgatagca
     3841 taaccctcag gccagaagga acagctggct gtgttcgtgc ctgtatagag catggtttat
     3901 tgcataatca acaacaaaaa ttatggtact taggccctat gtttcgtcat gaacgtcctc
     3961 agaagggtag ataccgtcaa tttaaccaat ttggtgttga ggcgctagga attactggca
     4021 ctgccattga gcttgagctg atctcaattt gccgcagatt atggaccgac ctgggttttt
     4081 ctcagtcagt tcaattacaa gttaattcat taggtgaaat aaatgaacgt cagaaatatc
     4141 gctctatctt ggtagagtat ctacacgatc attttcatat acttgatgaa gacagtaaga
     4201 ggcgattgga taaaaatcct ttgagagttc ttgatagtaa aaaccctgac cttcaacatc
     4261 tcattcaaaa tgcccctaag ttaattgatg ttcttggtga tgatagcaga gagcactttc
     4321 aatcgttctg caacggattg gaaacacttg gtattcctta ttccattaac cctgtattgg
     4381 taagaggatt agattattat ggccaaacgg tgtttgaatg ggtaacagac caattaggaa
     4441 gtcaagctac aatatgtgca ggaggcagat atgatatgtt ggtggagttc cttggcggag
     4501 cacccacacc tgcagttggg tttgctttag gattagaaag aatatttctt ttaatggaaa
     4561 ctttaaactt gttgaatgaa agtaacaaca agcaatcaat atttatcatt gctactaatg
     4621 aagaggccat attgaaagcg cttgtcatgg cagagtcaat tcgaaatgct catccatctc
     4681 ttgatgtcat taccaatacg gcaggcggtg gttttaaaag ccaatttaaa aaggccgata
     4741 aaagcggggc tcgtatggct ttgattcttg gggaagatga aatagccagg gaatatgtaa
     4801 gcattaaaga tttgagaact gaaatagaac aaatatcaat accgatgacc aaaataaatg
     4861 aatttttaca aaattatcta gcttaagggc aggagaatat tatatgtctg tttacatgac
     4921 ggaagaagaa caattagaga ttataaaaaa atggtggaag cgatatggca atatgataac
     4981 tattttttta tcaatagtgc tcctgattat cgcagggtac agatatatga attggcacaa
     5041 tgacaagata aaacaacaag catctattgc ctacgaaaat atgatgattg cattttccaa
     5101 tcaaaatata aagtctgttc gttcctatgc caatgaatta attaagaatt ataaccacac
     5161 tgtttatgct gatatagctc atatgacact tgcaaaaatc tacgtaagta aaaataaact
     5221 tgagcaagca agaaatgaat taaaggctgt tgcatcggaa agccaaatga caccgctaag
     5281 acaaattgcc aaaatccgca ttgcacgttt actggcggca gataaagctt ataccaatgc
     5341 attaaatgaa ttaagtgctg ttgaggacga agcctacttg ccagtaatta atgaactgaa
     5401 aggagatatt tatagcgcca aaggccaata tcaggacgcg atgaatgcct accgacttgc
     5461 aatagatgaa gtaagaacaa atggaatggg taatttattt ttagaaatga aaacaaatga
     5521 gttagcaatt aagactcaat ctatgattac tgatgataaa aaggtaaaag cagcttaatt
     5581 ttattataaa ggtttgtttt ttatgaagat tagaatatta gttttaatac tatgtgcctt
     5641 aactcaagga tgcacatatg tggatgatta tatgctcggt aaggataata cgccccaacc
     5701 caaagagcta aaagaaattc agcccaaggt taaaatggca caaagctgga caactccagt
     5761 tggaaaggct cataaaacaa atgagtattt aaatatcaaa ccagctattc gtggcgatgt
     5821 aatttatact gcagatgcaa gtggattggt tcaggcagtt aacaggaaag atggccagat
     5881 aaagtggtct accgcattaa aaaataatat agttagtgga ccaactgtag ctgctggtta
     5941 tgttgctgta ggaactaatg cctccacatt ggtactactt aatcaatcgg acgggaaaga
     6001 gatatggcag aataaagtat ctgccgaagt attagcccct cctgccatct cacatcagaa
     6061 ggtcattgca aaaaccattg atggtaaagt gtatgcgatt gatgcggtaa acggcaagca
     6121 actgtgggtt gcagatcatg gcgctcccag cttggtgtta aaagccagtt cgtctcctat
     6181 cattgtcgac gatctggttc ttgtcggatt ttccgatggc aaattagatg ctttggaatt
     6241 acagaccgga cgattaatct ggcaaagaag cattgcttat ggaacaggag ctagtgatgt
     6301 cgaacgttta gttgatattg attctgaccc tataataagc aataacgtcg cctatttggc
     6361 aacttatcag ggatatgttg gcgcactatc actgtctaat ggccaattca tatggagaaa
     6421 gccagcatct gtttataaaa atatgctttt aagtcataat aatctgtatt ttacggacag
     6481 caatgatgtc ttgtggtctt tgaatagtag cacaggacaa gtcaactgga agcaaacctc
     6541 cctaaaggca agaggactta ctgcacctgc attagttgga ggaaatttag ctgttggtga
     6601 taaaacagga tatttacata tcctatcaac tcaaacaggt gagttacttg gacgttctca
     6661 gctttctggt ggagtaacag tatcaccgag tgtttctggt aaaaatatgt atgtattaac
     6721 caataatgga atgctcaatc aactttcagt gagctaataa aatgatccct gtcatcgctt
     6781 tagttgggcg tcccaatgtt ggaaagtcta ctttgtttaa tagaattact aaaacgcagg
     6841 atgctcttgt cgcagatttt cctggcttga caagggatag acaatatgga cacgctcaac
     6901 atgaaaacaa gtcctttatt attgttgata ccggaggcat aggagttgat gacattgaag
     6961 tagatacttt gatgtcaaga cagtctcaag ttgctttaaa cgaagcaaat gtgattcttt
     7021 tcctagtcga tggccgttca ggactaacag gtatagatca gcaaattgca caagcattaa
     7081 gaaaacttaa taaaaaggta catcttgttg tcaataaaac agatggaatg aatgaagata
     7141 ttgcttgtgc cgattttcaa tcattaggca ttactgatgt ccatgctata tctgcatctc
     7201 atggtggagg catcagttca ctactcgaag aaattctcga gccatttaca acagaaacgc
     7261 atgaagcaac tgatgataaa gcgatcaaaa ttgcttttgc ggggcgccct aatgttggaa
     7321 aatcaacttt gataaacagg atattagggg aagaaagagt agttgtttat gacatgcccg
     7381 gaacaactcg agatagtatt tcaataccct ttacaagaga agataagcaa tatgtgctta
     7441 ttgatactgc tggtgtacgg cgtaagtctc gaattgatga aaaaatagaa aagttttctg
     7501 tcatcaaaac tctgcaagct ataaaagaag ctcatgtctg tttgttactt cttgatgcaa
     7561 atgaaggcat tacagatcaa gatatgaatt tgctcggttt tattattgaa tctggtaaag
     7621 cactagttat tgctgtgaat aaatgggacg gcctggaaga agatcataaa gaaaaaataa
     7681 agtcagaact gtcaagaagg ttgcattttg caaattttgc aaaaatcaga tttatttcag
     7741 cattacatgg gagcggggta ggtggattat ttaaagatat taatgaagcc tatcattcag
     7801 caatacaatc cttttctact ccaaaactaa ccagattatt acaagatatc agcgcaaagc
     7861 atacaccacc atgcatcaat ggccgacgaa taaaattacg ctatgctcac cttggcggac
     7921 acaatcctcc agtcattgtt attcatggaa atcaactcga tgccttgcca gaaagttata
     7981 aacgttattt aaacaatgag tttattaagc atttaggatt agtaggtact cctttaaaaa
     8041 tcgagtttaa aggaggtcaa aatccatttg ccaataaaaa aaataaatta tcacaaaggc
     8101 aagtgaacaa gaagaaacga ttaatgcgat gggcaaaaag caagaaatag tactccggtt
     8161 cagctcagcc aggtttaaat tgttctaatg aaaaaaaggt atcaattggg taacagtctc
     8221 ttcttattaa tctgctttga gtgttaatac atcacaaacc gaatgattta ctacagcatg
     8281 cgctgtactt cctagaatag acggtaatcc tgtagcagag tgtgagccta ttatgattaa
     8341 ttggcaattc agatcttttg ctttgtttaa tatatgttct tttacggacc cgatttctac
     8401 aaattgttgt tcctgaggta tttgtaaact ttctccaatt aatgataata cagtttgggc
     8461 atcctctttt gatgggttag ctagctccgt aaaacccaat ccttgagcta ttaatatact
     8521 tgttggcaac tcaatcacat gtaatagata taattttgca ttaaactgtt tagcaatatt
     8581 agctgccttt tctgtaaggt gactatgctc atttaacaaa tctgtggcaa ataaaatatt
     8641 tgtatacatg ttaacctcat tttaaataga atcattaagt tatagtgtag tagattatga
     8701 tatctatgtg taatttggta tttttaaatt agagtttaat tctcttagag gaacaagaac
     8761 aaaatctcgt aaaaggaaac aaggatgtgg aacgattaag tcttttgtgc gaattaatct
     8821 ataaccatat aaaatgatat caatatctaa tgttcttggc ccccaaaatt ttctcctgac
     8881 tcgcttgtgc ctgttttcta ttttctgaca ccacttaagg agcttatggg gtaacagagt
     8941 agttcgtatc tctataacaa cattacaaaa ttcctgttga ttttcaaagc cccaggcctt
     9001 attccaatat aagcgggaca ccttgataag gcatgttgat ggaatctgtt tgatttcttt
     9061 aatggcttgt cttatttgac gctcaggatt tttttgattc gatcccaagc tcagatagca
     9121 aacattcatt ttgctatttt ccgtttacga cgttttttag gccgcccggg cgttgttgaa
     9181 gccaattggg ttaccatgtt atttttttca acatcatcga cttcttgaaa cgtggcccac
     9241 cactgcgcca gctccattga ctcatcacca gataatgccc taagagccat aaaatcatac
     9301 gctgctctga atcggggatg aagaagtaaa ttaaatgctc tgccaccaaa tcttttgtcg
     9361 aagcgatatt gcaaaagcca aatttccctt ataacctggc tatacctttt aggtatcgaa
     9421 ataattgcat tctgttcact aagcactata gacatggctt tttcaatcga gggtaaaggc
     9481 ggaagcccag attcttgcag ttttctggaa cactctatca ttggaaacca taacaaaaca
     9541 gcaataagaa atgccggagt aacaggcttt ccatcacgta ttcttgtatc agtattttca
     9601 agtgctatgc ccaaaagcgg attaaccggg taatgactat caaatagagc agctgtttga
     9661 ggaaatagat aattaaataa accatactcg accaataatt tttgaaccga ttcggcttct
     9721 ccgcattgat aaagtttcgt tatttcatca aatagtcgcg aactggaaac atgggttata
     9781 agatggctta ttttggggaa gggagctttg gtctccggag ataaattaaa ttgcagcttg
     9841 gcactaaacc tgacagcacg taacatacgg acagggtcct cttgataacg cgtaacggga
     9901 tcgccaatta ttcgaattaa cttttgctta acgtcttcta caccactagt aaaatcaatg
     9961 attgaagaat cagaaatatt ataataaaga gagtttattg tgaaatcacg tctccatgcg
    10021 tcttcgtcca gagaaccata aacattatct cttacaagca tgccgcgttc attaaactgg
    10081 tggcttgcat cgcttgaatt atggccgcga aaagttgcta cctcaataat ttcacgatga
    10141 aataaaatat gtactagttt aaatcgacga ccaatgatac gagcattttt gaaaagattt
    10201 tttacctcat taggagtagc atttgtggcg atatcaaaat ctttaggcgc tttatggagc
    10261 agtaaatctc tgacactgcc tccaacaata taggcctgaa aaccatggct gattaaacgg
    10321 ttaagaactt taagcgcatt attacttata tctgctttcg atatgttatg tttatttctc
    10381 gggatgatat aagccaagtc aattgcatgt ccatgcgacc tgtttcttcg taacagtttt
    10441 ttaataaact tgatgattgt tgtttctctc ttaattgatt aaacagtaat tcagtagtgt
    10501 tttcaattat cctgcaagtt taagatttac tttacagaaa tattcaattc tataaaactt
    10561 aacacgtaaa cttttcagat gactatttaa tctaaattgt taacaccaag caattataac
    10621 gaagcaaatg atcaaagtga atcatttacc tttacaaata ttttagagaa aataatatat
    10681 cttgcatttt attaggataa taaatggatt ttattgatat aggccttagt ttaactgctt
    10741 tgattattct tgaagttgtt ctaggaatag ataatctggt tattctatcg attcttacag
    10801 aaaaattacc acgtgaaaaa agaaaaaagg cgagaagatg gggcttaaca tttgcctgga
    10861 tgacacgact tcttttacta ggctcagcag tttttttagt taagttagta aaaccaattc
    10921 tgacaatagg cagtctggta ttttctgctc gtgatctgtt cctcatatta ggaggtgctt
    10981 ttttaatctg gaagtcaact gatgaaattc atcaggacgt tatgaatgaa ccattaatgg
    11041 aacctgtttc aaaaaatgtt tcttcatctg ccactttcag gggagttgtt attcaaatcg
    11101 cattgcttga tattatattt tcactagata gcgttctcac agccgtaggg ttaacatctc
    11161 attttatggt catggcagtg gcaatcacca tcgcaattat cattatgatt tgggcgagtg
    11221 agcctgtaag taaatttatt agcgaacatc ctaccattaa aatgctggcg cttagttatt
    11281 tgattttaat aggaaccgtt ttggtagctg atggttttga attccatgtg cccaggggat
    11341 acttgtattt tgctatgggt ttttcattag cagttgaatc actcaacttg attaaaaatg
    11401 aacgtgtaaa gcgtaaaaaa ttaaaaagag agtaagtatg ttatttgtta aagcttttca
    11461 tattatcgcc ttggtagcct ggttcgcggg gttgttttat ttaccacggc tctacgtata
    11521 ccacgctgaa acactagata aaattagcgt tgatcggttt aaaataatgg aaagaagatt
    11581 atattacgga attacatggc ctgcagctct tgcaaccaca tttcttgggt tgtggctatt
    11641 cacttacaat ttatcttatt atcttaaagc aggatggatg cacatgaagc ttgcattggt
    11701 tttgctctta tgggtatatc atttaacttg cggccacttc cttaaatcat tcgccctcgg
    11761 aaagaataca agaagctctc gtttttatcg attttttaat gaaatgccta cattgctgct
    11821 ggtaggcatt gttttgttgg tggttgtaaa accattttaa tccatttcaa ccatttcgaa
    11881 atcttctttt ccagcaccac aatcaggaca aaaccagttt tcaggaacat cttcccatcg
    11941 tgtgcctggc tcaataccat cttctggcca accttcagcc tcatcataaa taaaaccaca
    12001 aataacacaa atgtatcttt tgtactcttg catagtctaa actcaataaa aaaagtgcta
    12061 atttatctac tgttgatgat tatgtaaagt taaaccttat ttaatatcac aataaatttt
    12121 aaattaaacc ttataatgaa cattataact tcatcgagaa ttgacatggg tttgctaaaa
    12181 tcaaccccaa aaaccagtca atgtggttat cttttaaaga caggcgctgc cactattttt
    12241 tgcaatattc atgaaatgat tgtaatattt tcttgcctca gggttatcag tctaatttga
    12301 ttagatagtt tttaataaaa
//
LOCUS       DAXLDT010000092        12201 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXLDT010000092 DAXLDT010000000
VERSION     DAXLDT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.092"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            135..1208
                     /locus_tag="ACF2SP_002033"
     CDS             135..1208
                     /locus_tag="ACF2SP_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Leu/Phe/Val dehydrogenase"
                     /protein_id="HFD2359813.1"
                     /translation="MMSVDNIKNDNQVLAEEDFLEYALSHGFGDLHFKVDPQTGMKAM
                     IAIHSTKLGPALGGCRFIEYPNTAAALKDAMRLARGMSFKAASVNLPLGGGKSVIIKP
                     KGFFNRAEYMHRFGEFVNELNGRYITALDSGTELSDMDIIAEHTDYVASLSRYNGDPS
                     PSTAKGVLRGIQAAVAFKLGKDSLKGLHIAIQGLGHVGYALAKQLHELGATLTVADIS
                     PAAVNMAVQELGAKAVGTDIIHKVPCDVFAPCALGAIINDLTIPQLQTSIIAGAANNQ
                     LAHTYHGKILHEKGILFAVDYVINAGGLIFAASKYLQTPEDKVSEQIDSIYTSLTEIF
                     TRSAKDNSPTSEIADTLAKEKLA"
     gene            1223..1879
                     /locus_tag="ACF2SP_002034"
     CDS             1223..1879
                     /locus_tag="ACF2SP_002034"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2359814.1"
                     /translation="MKHLSLTPELYEYLLDISLREHPALTALRKETSTMELANMQVAP
                     EQAQFMQMLIRSTRAKKVLELGTFTGYSALAMSLALPDDGQVITCDINEGWTKHAHPY
                     WREAKQEHKIKLRLGPALDTLHSLLNEGGEHQFDFIFIDADKTNYLNYYELALKLVTP
                     KGLIAIDNIFWDGKVIDPNDTSGQTREIKKLNQVIKNDSRVFVSLLAIADGMFLVQPI
                     "
     gene            1946..2992
                     /gene="hppD"
                     /locus_tag="ACF2SP_002035"
     CDS             1946..2992
                     /gene="hppD"
                     /locus_tag="ACF2SP_002035"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HFD2359815.1"
                     /translation="MQNNNPCGLDGFAFLEFSGPDRNKLHQQFSEMGFQAVAHHKNQD
                     ITLFKQGEIQFIVNAASHCQAEAHASTHGPGACAMGFKVKDAKAAFQHAIAHGGIAFQ
                     DAPHANHGLPAIQAIGGSVIYFVDEEHQPFSHEWNITSPEPVIGNGLTAIDHLTHNVY
                     RGNMDKWASFYASIFNFQEIRFFNIKGKMTGLVSRALGSPCGKIKIPLNESKDDLSQI
                     EEFLHEYHGEGIQHIALNTKDIYKTVNGLRKQGVKFLDVPDTYYEMINDRLPWHKEPL
                     NQLHAEKILIDGEADPKDGLLLQIFTENIFGPVFFEIIQRKGNQGFGEGNFQALFEAI
                     ERDQVRRGTLKELT"
     gene            3048..4046
                     /locus_tag="ACF2SP_002036"
     CDS             3048..4046
                     /locus_tag="ACF2SP_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFD2359816.1"
                     /translation="MKLASLKSTRSRDGELCIVNRLLTAAIRVPHIASTLQYALDHWD
                     VVEPKLQLVYQQLNDNQIEDAFAFAPELCASPLPRAYQWADGSAYVNHVELVRKARGA
                     EMPADFWTNPLMYQGGSDAFLGPRDPILVSSEAYGIDFESEVAVITDDVPMGIDHTNA
                     AHHIKLLMLVNDVSLRNLIPEELAKGFGFFQSKPASSFSPVAITPDELGSYWDGQRVN
                     LPLISHFNGQLFGQPNAGIDMTFSFPELIQHAAKTRFLTAGTIIGSGTVSNLDRSKGS
                     SCIAEKRMLEMIEFGQAKTPFMRFGDRIRIEMLDGQGYSLFGSIDQIVTKYEKDSL"
     gene            4043..4681
                     /gene="maiA"
                     /locus_tag="ACF2SP_002037"
     CDS             4043..4681
                     /gene="maiA"
                     /locus_tag="ACF2SP_002037"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFD2359817.1"
                     /translation="MILYDYFRSTACYRVRITLNLKKIVYEKIEVHLVNNGGEQHSLQ
                     YHQINPQELVPSLDINGHILSQSMAIIDYLEEVYPEIPLLPKDPFMKANLKSLALIVA
                     CDMHPLNNLRVLNRLKEQFRASQEQVLEWYHHWLKTGFDAFEEKLGTMKRDKPVCLGS
                     EVSLADVCLIPQVYNAQRFHFDMASYPIINQINEYCLTLPAFHDAAPEAISS"
     gene            complement(4678..5238)
                     /locus_tag="ACF2SP_002038"
     CDS             complement(4678..5238)
                     /locus_tag="ACF2SP_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359818.1"
                     /translation="MLQRVLLVLLCFFSFLASASDTVLKLYRPFGDVFEQAPPVVKKI
                     MSGQCYEQSRIIIREDAWRCQAEDQLFDPCFVKAGSKKMEAVCPQSPWVGDSVQINVG
                     IPLNNEHHKTLDMSRAFPWAIELVNGEYCLAVNSNEQYDSMPVRYRCSNQNVLIGYLQ
                     RCKTAWSMLEKTPKGVITVELRRAWF"
     gene            5386..6789
                     /gene="asnS"
                     /locus_tag="ACF2SP_002039"
     CDS             5386..6789
                     /gene="asnS"
                     /locus_tag="ACF2SP_002039"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFD2359819.1"
                     /translation="MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHD
                     GSCFSPIQIVATDQLSNYHKEVTKLTAGCSMIATGKLVASQGKGQFFEIQAESIEVVG
                     WVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
                     VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVE
                     AYCMAMSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCK
                     TVLEERADDMDFFNQFVAPGCIERMEHIADSEFEIMTYTDAVKALEASDQKFEFPVSW
                     GLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVS
                     NVRDVIPFPRTPGHADY"
     gene            complement(6786..7184)
                     /locus_tag="ACF2SP_002040"
     CDS             complement(6786..7184)
                     /locus_tag="ACF2SP_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359820.1"
                     /translation="MDEKKDKTIQINEITSTVEDEKKAQPLRKSERSLPVKSHRRSFS
                     IFYPYSAENSKISPSAQTSNKGFLPGPDFNREHYDTVDAGGPLAGNADDKILEKIFSP
                     RNRRRLLPESHQPQQQTVEVDDDSFRPLPI"
     gene            complement(7208..7891)
                     /gene="lolD"
                     /locus_tag="ACF2SP_002041"
     CDS             complement(7208..7891)
                     /gene="lolD"
                     /locus_tag="ACF2SP_002041"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFD2359821.1"
                     /translation="MNDIILTSQKLYKSYHDGTSRVEVLKGVDLAITKGDRIAIIGPS
                     GSGKSTLLHLLGGLDKPTSGLITLGKVDWQKINEKQRCQLRNQQLGFVYQFHHLLPEF
                     TALENVMMPLLLAGMAVKDAEEKAINMLEQVGLKPRLAHKPAQLSGGERQRVAIARAL
                     VHQPHCVLADEPTGNLDEATASKVFDLMLELNKKMNTALVIVTHDQRIAERMDRVLVL
                     HEGSLYARE"
     gene            complement(7884..9131)
                     /locus_tag="ACF2SP_002042"
     CDS             complement(7884..9131)
                     /locus_tag="ACF2SP_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFD2359822.1"
                     /translation="MYKPLALFIGLRYTRSRKKNHFVSFISLSSMLGIGMGVMVLITV
                     LSVMNGFDQEIHRRFFGMAPEITITGPDERLSDWPEVVKKVETIPGIKAIAPYVGSQG
                     LLTHEGQVLPIVLTGILPEKEQSVTHLNKKLLAGNMDNLKHFGIILGKGLADSLGVMI
                     GDKVTIMIPQATVTPAGMIPRFKRFTVVGVFSAGTGFNFDTKLAFINIEDAQKLMQMD
                     KNDVSGIKMKINNVYKAPELSYELSDLLGEGYQVGNWTQQFGAFFEAVKMEKTMMFMI
                     LLLIIAVAAFNLVSSLVMVVNDKQAEIAILRTIGATPSTILWVFIVQGMMVGLVGTIL
                     GLLGGLVLANNATEIVNALQSFFQVKVLSSSIYFVDYLPSKIMFRDLWQVCAMALLMS
                     FAATIYPAWRASKTVIAEALHYE"
     gene            complement(9583..9669)
                     /locus_tag="ACF2SP_002043"
     tRNA            complement(9583..9669)
                     /locus_tag="ACF2SP_002043"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9633..9635),aa:Leu,seq:taa)
     gene            complement(9687..9760)
                     /locus_tag="ACF2SP_002044"
     tRNA            complement(9687..9760)
                     /locus_tag="ACF2SP_002044"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9726..9728),aa:Cys,seq:gca)
     gene            complement(9821..9896)
                     /locus_tag="ACF2SP_002045"
     tRNA            complement(9821..9896)
                     /locus_tag="ACF2SP_002045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9861..9863),aa:Gly,seq:gcc)
     gene            complement(9949..10512)
                     /gene="pgsA"
                     /locus_tag="ACF2SP_002046"
     CDS             complement(9949..10512)
                     /gene="pgsA"
                     /locus_tag="ACF2SP_002046"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFD2359823.1"
                     /translation="MSTLTSLPNILTLMRISIIPVFIMVFYFPFKWAHPTAAVLFAIA
                     SITDWLDGYLARKLEMMSHFGAFLDPVADKLLVSTSLLLLVGAKDIDYITIPAIVIVG
                     REIVISALREWMAEVGSRASVAVGYIGKVKTSLQMIALVLLLAFNPLVSWWGSLGFVL
                     LYVSAILTIWSMVIYLAIAWPELMKKS"
     gene            complement(10533..11192)
                     /locus_tag="ACF2SP_002047"
     CDS             complement(10533..11192)
                     /locus_tag="ACF2SP_002047"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD2359824.1"
                     /translation="MSSAYQLVIFDWEGTIADTLGVILHTVATEAKMLGFGDIDPYQA
                     RKYVDLGLVQAVKKTLPNLTLRQQEDLLQAVQSAMISRPTEVRLIPGVLEFIQQLHQA
                     KVDLAIATNKGHNSLIRALQATELDQLFKVTRSAGQVPAKPCPQMLEEIMEEFGRDPS
                     STLMIGDSTTDMEMAKRINVNAIGMDFYHQQEDALKAAGALAVFDDYKLLSAYLMLPE
                     A"
     gene            complement(11185..12132)
                     /locus_tag="ACF2SP_002048"
     CDS             complement(11185..12132)
                     /locus_tag="ACF2SP_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFD2359825.1"
                     /translation="MSEVSYKEIGSEEDGQRLDNYLIRILKGVPKSHIYRIIRGGEVR
                     INKKRAQANSRLQIGDSIRIPPIRMTDSKEIFVSDNLAKRLKDSIIFEDSSLLVINKP
                     AGIAVHGGSGLSLGVIEALRKTRHDLSYLELVHRLDRETSGCLLLAKKRSTLRAIQAL
                     LEAREVQKIYWALLTHRWDGKKKITITAPLEKNTLKSGERVVSVQKDGKASETDFKLL
                     ENYHQACWVEASPKTGRTHQIRVHSAYFGHVIVGDGKYGSKVGEVEGIDYQQNRLYLH
                     ARSIQFNLNGVMHVFQADVDDGFGNTLKQLRARSLLSYE"
BASE COUNT         3672 a         2579 c         2360 g         3590 t
ORIGIN      
        1 aagtacatga gcatttttta gaatgttttt ttctcaagat gcgccaaata cggccgtttg
       61 ctaagaatta cattgattta attgacgaat agatctataa cttctcccaa aattattgca
      121 agaggagctt aaaaatgatg tctgttgaca atattaaaaa tgataaccag gttttggctg
      181 aagaagattt cctcgaatac gcattgtctc atggatttgg tgatttacat tttaaggtgg
      241 atccgcaaac ggggatgaag gctatgattg caattcatag caccaagttg ggacctgctt
      301 tgggtggttg tcgatttatt gaatatccaa atactgctgc tgctttgaag gatgctatgc
      361 gccttgccag aggcatgagc tttaaggctg cttcagtcaa tttacctttg ggcggtggaa
      421 aatcggttat tattaagcct aaggggtttt ttaatcgagc agaatacatg catcggtttg
      481 gtgagttcgt taatgaattg aatggcaggt acatcactgc tttggatagc ggtactgaat
      541 taagtgacat ggatattatt gctgagcata ctgactatgt tgccagttta tccagataca
      601 atggagatcc ttcaccttct actgctaagg gtgtgttaag agggattcag gctgctgttg
      661 cttttaaatt aggtaaagac tctctcaaag gtttgcatat tgctattcag ggtcttggcc
      721 acgtcggtta tgctctggca aaacaattac acgaattagg cgcgacatta actgttgctg
      781 atatttcacc tgctgctgtg aacatggcgg ttcaagagtt gggagcgaaa gcggtcggca
      841 ctgacattat ccataaagta ccgtgtgatg tatttgctcc ttgtgcttta ggtgcgatta
      901 tcaatgatct tactattccc cagttacaaa ccagtattat tgctggcgct gccaataatc
      961 agctcgcaca tacttaccat ggcaagattt tacacgaaaa aggcattctg tttgctgtgg
     1021 attatgtgat taatgcaggt ggtttgatat tcgctgcctc taaatactta caaacgcctg
     1081 aagacaaggt aagtgagcaa attgatagta tctatacctc tctgactgaa atttttactc
     1141 gctctgctaa agataattca cctactagcg agattgctga cactttggct aaggaaaaat
     1201 tggcttaatt tggataccgt ttatgaaaca tttatccctt actcctgagt tgtacgaata
     1261 cctgcttgat atttccttgc gtgaacatcc tgctcttacg gcattacgca aagaaacatc
     1321 cactatggaa ctcgccaata tgcaggtagc tcctgagcaa gcccaattta tgcagatgct
     1381 gatacgttcg actcgtgcaa agaaagtatt agaacttggg acattcacag gttatagtgc
     1441 cttggccatg tcccttgctt tgcctgatga tgggcaagtt atcacctgtg atattaatga
     1501 gggatggaca aagcatgccc atccttattg gcgtgaagcc aaacaggaac ataaaattaa
     1561 attacgcctt ggacctgctc ttgacacttt acactcatta cttaatgaag gtggagagca
     1621 tcaatttgat ttcattttta ttgatgctga caaaactaat tatctgaatt actatgaact
     1681 ggctttaaaa ttagttacac ctaaaggatt aattgccata gataatattt tttgggatgg
     1741 aaaagtaatt gatccgaatg atacgagtgg acaaaccaga gaaattaaaa aattaaatca
     1801 agtgattaaa aacgattcaa gagtctttgt cagtttgttg gctatagctg atgggatgtt
     1861 tctggtgcaa cccatttaat ctttaaaatc cccggcaagc aatacactgc attgctatgt
     1921 aaataagtac aaggagaatc tgataatgca aaataacaac ccctgcggat tagatggctt
     1981 tgccttttta gagttttcag gccctgatag gaataaatta catcaacaat tttctgagat
     2041 ggggtttcag gccgttgccc accataaaaa tcaagacatt actcttttca aacaagggga
     2101 aatacaattt atagtgaatg cggcttccca ttgtcaggca gaagcgcatg cttcaactca
     2161 tggtccaggc gcttgtgcaa tgggctttaa agtaaaagat gccaaagccg cttttcaaca
     2221 cgctatcgcg catggcggta tagcatttca ggatgcacct catgccaatc acggcttgcc
     2281 agccatccag gctattggtg gtagtgttat ttattttgtc gatgaagaac accaaccctt
     2341 ctctcatgaa tggaatatta cctcaccaga acccgtaatt ggaaatggtc tgaccgcaat
     2401 cgaccatctc acccataacg tttatcgcgg taatatggat aaatgggcca gtttttatgc
     2461 ttccattttt aacttccagg aaattcgttt tttcaatatc aaaggaaaaa tgactggttt
     2521 ggtcagtcga gcattaggta gcccttgtgg caaaatcaaa attcctttaa acgaatccaa
     2581 agatgattta tcacaaattg aagagtttct tcatgaatat catggcgagg gcattcaaca
     2641 catcgctctc aataccaagg atatttataa aacagtcaac ggcttaagaa aacaaggggt
     2701 caaattcctg gatgtaccgg atacttacta tgagatgatt aatgaccgcc tcccatggca
     2761 taaggagcca ctgaatcaac tccatgctga gaaaatttta attgatggag aagcagatcc
     2821 caaagacggc ttgttactgc aaatatttac tgaaaacata tttggaccgg tcttttttga
     2881 aattattcaa cgcaaaggca atcaggggtt tggtgaaggg aatttccagg ctctattcga
     2941 agctattgaa agagatcaag ttcgacgcgg tactttaaaa gaattaacct agacaaacag
     3001 ttgaaatgta atgatgctgt ttttcaggca taagccagga gatctatatg aaactcgcca
     3061 gtttgaaatc aactcgttct cgggatggag agctttgcat tgtcaacaga ttgctgaccg
     3121 ctgcaatacg cgttcctcat atcgcatcaa cacttcaata tgccctggat cattgggatg
     3181 ttgttgaacc caaattacaa ctggtttatc aacagttaaa tgataatcaa atcgaagacg
     3241 cctttgcgtt tgctcccgag ctatgtgcct cgccattacc ccgtgcttat caatgggccg
     3301 atggcagtgc ctatgtaaac catgtcgaat tggttcgtaa agccagagga gcagaaatgc
     3361 ctgctgattt ttggaccaat ccactcatgt atcaaggcgg atctgatgct tttctggggc
     3421 ctcgtgatcc tattttggta agctcagaag cctatggaat tgactttgaa tccgaggtgg
     3481 ctgttatcac tgatgatgtt cccatgggaa ttgatcatac aaacgctgcc catcatatca
     3541 aactgttgat gttggttaat gatgtctctc tacgcaatct tatacccgag gaactggcaa
     3601 aagggtttgg gttcttccaa tcaaaacctg ccagcagctt ctccccggta gcgataaccc
     3661 cggatgaact aggttcttac tgggatggac agcgtgtgaa tttgccatta attagtcatt
     3721 ttaatggaca attatttggc caacccaatg ctggaattga tatgaccttt tcttttccag
     3781 aactgataca gcatgcggca aaaacaaggt ttttaactgc gggcactatt ataggttctg
     3841 gaacagtatc caaccttgac cgcagcaaag gttcttcttg tattgctgaa aagcgaatgc
     3901 tggaaatgat agaattcgga caggcaaaaa caccgtttat gcgatttggg gaccgaattc
     3961 gcatagaaat gctcgatggt caagggtatt ccctatttgg ctccattgac caaatagtga
     4021 cgaaatacga gaaggattcc ttatgatatt gtatgactat ttccgctcta ccgcatgcta
     4081 tcgagtaaga atcactctta atttaaaaaa aattgtttac gaaaaaattg aagtgcatct
     4141 ggttaataat ggaggcgaac agcatagcct tcagtatcat caaattaacc ctcaagaact
     4201 tgttcccagc ctcgacatca atggacatat cctgagccaa tcaatggcaa tcattgatta
     4261 tttggaagaa gtctacccgg aaataccatt gcttcctaaa gatcccttta tgaaagccaa
     4321 tctaaaatct cttgctttaa ttgttgcttg tgatatgcat cccctcaata atttaagagt
     4381 gttgaatcgc ttaaaggagc aatttagagc cagtcaagaa caggtattgg aatggtatca
     4441 tcattggcta aaaacaggat ttgatgcttt tgaggaaaaa cttgggacta tgaagcgcga
     4501 caagccagtc tgccttggaa gtgaggttag tctggcagac gtttgtttga tcccccaggt
     4561 ttacaatgcc caacgatttc attttgatat ggcgagttac ccaataatca atcaaatcaa
     4621 tgagtattgc ctaaccctgc cggcattcca tgatgcggca ccagaagcca tttcatccta
     4681 aaaccatgct cttctcaact ctaccgtgat cacgccttta ggggttttct ccaacatact
     4741 ccaggctgtt ttacatcgtt gcaggtaacc aattaaaaca ttttgattgc tgcaacggta
     4801 ccttacaggc attgaatcat attgctcgtt agagtttaca gccaaacagt attcaccatt
     4861 aaccaattca atagcccagg gaaaagcgcg agacatatct aaggtcttat ggtgttcatt
     4921 attcaaaggt atccctacgt taatttgtac gctatcacca acccatggag attgggggca
     4981 tacggcctcc atttttttgc taccagcttt cacaaaacaa ggatcaaata attggtcttc
     5041 agcctgacaa cgccatgcgt cttcgcgtat aatgatgcgg gattgttcgt aacattggcc
     5101 agacattatt tttttaacga caggtggtgc ttgttcaaaa acatcaccaa aaggtctgta
     5161 taattttaga acagtatcgc tagcgctggc taaaaaagaa aaaaaacata gcaatacgag
     5221 taaaactctt tgcagcatgg tgttccccca cggattttat ttagtgtatc atcacatttt
     5281 aattcagggt ttaggaaata ctacaaacgg gagaaaatca atccctcgaa ggattggaat
     5341 atttcctaag ccctgaatca atcgttttcg attttggagt atataatgac agaagtcttt
     5401 actataaaac aatgtctcga cggcgaaatc agcattgatg aaaccgttac ggtaagagga
     5461 tgggtgaaaa ctcgaagaga ttcaaaagct ggcttgtctt tcatcagcct tcatgatggc
     5521 tcttgctttt ctcccataca aatcgtcgct accgatcagt tatccaacta ccacaaagaa
     5581 gtaacaaaat taactgctgg ctgttcaatg atagcgactg gaaaattggt ggcatcgcaa
     5641 ggaaaaggac aattttttga aatccaggcc gaatccattg aagtggttgg atgggttgaa
     5701 aacccagata catatcctat tcaagccaaa cgccatactc ttgagttttt aagagaggta
     5761 gctcatttgc gccccagaac caataccata agtgctgtca ctagagtgcg acattctttg
     5821 gcacaagcca ttcaccgttt ttatcatgag caaggttttt tttgggtgca tacacctatc
     5881 attactgcca gtgactgcga gggggcagga gaaatgttca gagtatccac tctggattta
     5941 ttaaatatcc ctaaaaatga taaaggacaa atcgatttca gtaaggattt ttttggtaga
     6001 gaaacgttct taactgtttc tggccaacta aatgtcgaag cttattgtat ggccatgtcc
     6061 aaggtctaca cttttggacc aacatttcgg gctgaaaatt cgaatacaag ccgccatttg
     6121 gctgaattct ggatgatcga acctgaaatc gcttttgcaa atctggaaga catttgtaag
     6181 ctcagccaaa gtatgttgcg ttatctctgc aaaacagttc ttgaagagcg tgccgatgat
     6241 atggactttt tcaatcaatt tgtcgcgcca gggtgtatag aacgtatgga acatattgcg
     6301 gattctgaat ttgaaattat gacctatacc gatgcggtaa aagcacttga ggcgagtgac
     6361 caaaaatttg aatttccagt tagttgggga ttagatctcc aatcagagca tgaacgttac
     6421 ctggctgaag tgctttgcaa aaagcctgtc attgtgacaa attacccgca agaaattaag
     6481 gggttttaca tgcgtcttaa cgacgatggg aaaacggtag ccgctatgga cgttttagct
     6541 ccaggcatcg gagagattat cgggggcagc caacgcgaag agcgattgga aatactggat
     6601 agacgcatgg acgaatgcaa cctgaataaa gaacattacc aatggtatag agatctgcgc
     6661 cgttatggca ctgttcctca tgccgggttt ggcttgggct ttgaacgtct gattagctat
     6721 gttacaggag tatccaatgt gcgagatgtg atacctttcc ctcgcacacc aggtcacgcg
     6781 gattattaaa tcggtaaagg tctaaaagag tcatcatcaa cttcaactgt ttgttgttgg
     6841 ggttggtgac tctcaggcag caatcgccgt ctatttcttg gcgaaaatat tttttctaat
     6901 atcttatcat ctgcattacc cgcaagcggt cctcctgcat caactgtatc ataatgttcc
     6961 ctattgaaat caggtccagg tagaaatcct ttgttacttg tctgagctga aggagatatt
     7021 tttgaatttt cggctgagta aggataaaaa atagaaaaac ttcttctatg tgactttaca
     7081 ggtagactac gctcggattt tctcaatggt tgggcttttt tttcgtcctc aacagtgctt
     7141 gtaatttcat tgatctgaat tgttttatct tttttttcat ccattgttaa cactccttat
     7201 cttattttta ttctctcgca tacaaactgc cttcatgcaa aacaagtact ctatccatac
     7261 gttctgcaat ccgttgatca tgagttacta taaccagggc tgtattcatt tttttattaa
     7321 gttccaacat caaatcaaaa actttactgg ctgttgcctc atccaaattt ccagtcggct
     7381 catcagccag aacacaatgt ggctgatgca ccaaagctct cgctatggca acccgttgcc
     7441 tttctcctcc agacaactga gcaggcttat gcgctaatcg tggtttaagc cctacctgtt
     7501 ccagcatatt aatggctttt tcttccgcat ctttcacagc catacccgcc agtaataacg
     7561 gcatcatcac attctcaagt gccgtaaact cgggtaataa atggtggaac tgataaacaa
     7621 atcctaattg ttgattacgc aattgacaac gttgtttttc atttattttt tgccaatcga
     7681 ctttcccaag agtaatcagg ccgctggttg gcttatccag cccacccaac aaatgcaaaa
     7741 gggtgctctt tccagaacct gatggcccga taattgcgat cctgtcacct tttgttatgg
     7801 ccaagtcaac tccttttaac acctcgactc tagatgtgcc atcatggtag gacttataca
     7861 gtttttgact tgttaaaatg atgtcattca taatgcaaag cctccgcgat gactgtcttt
     7921 gatgctcgcc aggcaggata aatggttgca gcaaaactca ttaataacgc catggcacaa
     7981 acttgccaca gatcacgaaa cataatttta gagggcaaat aatcaacaaa atagatgctt
     8041 gaagacaata ccttcacctg gaaaaaggac tgcaacgcat taactatctc cgttgcatta
     8101 ttggccaaca ctaaacctcc taacagccca agaattgtac caaccaggcc aaccatcatt
     8161 ccctgtacaa taaaaaccca caaaatggta gaaggagtag cgccaatagt tcttaaaata
     8221 gcgatttctg cttgtttgtc attgactacc attaccagag aggacaccaa attaaaagct
     8281 gccacagcaa taataagcaa taaaatcata aacatcatcg ttttttccat tttaactgct
     8341 tcaaaaaaag ctccaaattg ctgggtccaa ttgcctactt gatatccttc tcccaaaaga
     8401 tcagataatt cataagacaa ttcaggtgct ttgtatacat tattaatttt catttttata
     8461 cctgaaacgt cattcttatc catttgcatt aatttttgag catcttcgat attaataaat
     8521 gccaatttag tgtcgaaatt aaatcctgta ccagcagaaa aaacacctac aacggtaaag
     8581 cgcttgaatc gagggatcat accggcagga gttactgttg cttgcgggat cattatcgta
     8641 accttatccc caatcatcac cccaaggctg tccgcaagac cttttccaag aataatccca
     8701 aaatgtttca gattatccat attacctgcc agtagtttct tattaagatg agttactgat
     8761 tgttcttttt ctggcaaaat accggtcaga acaataggca aaacctggcc ttcatgagtc
     8821 aataaccctt gacttccaac ataaggtgca attgctttta ttccaggaat tgtttcaacc
     8881 tttttaacca cttcaggcca atcacttaat cgctcatcag gacctgttat ggtgatttca
     8941 ggcgccatcc caaaaaaacg acgatgaatt tcctgatcaa aaccattcat tactgataaa
     9001 acagtgatta aaaccatcac tcccatgcct atacccaaca tggaacttag ggaaataaag
     9061 gaaacaaagt gatttttctt acgtgaacgc gtataacgta atccgataaa aagagccaat
     9121 ggtttataca taatcgtatt cagctggatt aaagttagta ttgtacagca cttatagaaa
     9181 aggccaagat tgaagcctaa agattttaaa gaacttatta ataattcatc atcagacaac
     9241 ccacacagtt atccacagaa tctgtggata tctaataagt cccattcatc gattagagta
     9301 tcaattaagc aaatgccaac atacataaaa tgaacatccg ttacgatata acagatgctg
     9361 cacataacca gaaaattatt aaatggaact ggtatttgtc agtgaaattt taaaattaat
     9421 taggaaatgc attatttaaa atttaaatgg agaaaagaaa ttgttaggat gcttcgtttt
     9481 acgaagaaca atgaaatgaa atgactaaag ggtttaattg gaatatgaaa ttgattcaag
     9541 gtggggttat cattgccctt taaaatgggg attagtcaaa tttggtgccc agggccggaa
     9601 tcgaaccggc atggtgttac caccgaggga ttttaagtcc cttgcgtcta cctgtttcgc
     9661 cacctgggca ttcactgttt ttttaatgga ggctgaggcc ggaatcgaac cggcgtacac
     9721 ggctttgcag gccgctgcat gaccactctg ccacacagcc tcaaatctat atactttgct
     9781 attgctctaa aaaaaaagcg acttcgatgt cgcttaaatt tggagcggga aacgagactc
     9841 gaactcgcga ccctaacctt ggcaaggtta tgctctacca actgagctat tcccgcgtct
     9901 ctacgggatg agattctacc gaaaataaat accctgtcaa cagcaaaatt aacttttttt
     9961 cattaactct ggccaagcaa tagccaaata aatcaccatg gaccaaatcg tcaaaatagc
    10021 agacacataa agaagaacaa accctaagct cccccaccag gaaacaaggg gattaaatgc
    10081 cagcaataat accaaagcga tcatttgcaa agaagtttta actttcccta tatatccaac
    10141 ggcaacacta gcacgacttc caacttcagc catccactcg cgcaaggctg aaatcacaat
    10201 ttctcgtcca acgattacga tagcaggtat agtgatataa tcaatatcct tcgctccaac
    10261 tagcaataac aagcttgtcg ataccaataa tttatcggcg acgggatcaa gaaaagctcc
    10321 aaaatgagac atcatttcaa gtttgcgcgc caaataaccg tctagccaat cagtaatgct
    10381 tgcaatagca aaaagaacag cagcggtagg atgagcccat ttgaaaggaa aataaaatac
    10441 cataataaat actggaatga tcgaaatccg cattaaggtt aatatattag gcaaactggt
    10501 taacgtactc actgctgaac atcccccctt ttttatgctt ccggcaacat cagataagct
    10561 gacaatagct tataatcatc aaatacagcc aaagcacctg ctgctttaag cgcatcctct
    10621 tgctgatggt aaaaatccat gcctatagca ttaacattga ttctttttgc catttccata
    10681 tccgttgtag aatcacctat catcaaagtt gatgaaggat ctctcccgaa ttcctccatg
    10741 atttcctcaa gcatttgcgg acaaggtttt gccggtactt gtccagcaga gcgtgtcact
    10801 ttaaacaatt gatcaagctc agttgcctga agagcacgga tcagagagtt gtggccttta
    10861 tttgtagcga tggccaaatc gacctttgct tgatgaagtt gttggataaa ttctaatact
    10921 ccaggtatta aacgcacttc agttggccgt gaaatcattg cggattgcac tgcctgtaac
    10981 aaatcttcct gttgtctgag agttagattg ggcaatgtct tttttactgc ttgaaccaat
    11041 cccaaatcta catattttct tgcttgatat gggtcaatat caccaaagcc caacattttg
    11101 gcttcagtag caacagtatg taaaataact cccaatgtat cagcaatcgt gccttcccaa
    11161 tcaaaaatta ctaattgata tgcactactc atagctcaat aggctccttg cacgcagttg
    11221 ctttaaagta tttccaaatc catcgtctac atcagcttga aatacatgca ttaccccatt
    11281 cagattgaat tgaatagacc ttgcatgcaa ataaagacga ttctgttgat aatctatacc
    11341 ttccacctca cccaccttag agccgtattt cccatcacca acaatgacat gaccaaagta
    11401 agcgctatga acgcgaattt gatgtgtccg tcctgtttta ggagaggctt ctacccaaca
    11461 ggcctgatga taattttcca gcaatttaaa atctgtttct gatgccttac catctttctg
    11521 aacggaaaca accctttctc cggatttcaa tgtgttcttc tcgagaggag ctgtaattgt
    11581 gattttcttt ttaccgtccc aacgatgcgt taataaagcc caataaatct tctgcacttc
    11641 gcgggcttcc aatagcgctt gaatggctcg taacgtgctt cttttttttg ctaataaaag
    11701 acaaccggaa gtctctctat ccaatcgatg taccaattcc aaataggata aatcgtgcct
    11761 tgtctttctt aatgcttcaa taacccccag gcttaaacca ctaccaccat gtactgcaat
    11821 acctgctggt ttattaatta ccaacaagga agaatcttca aaaatgatac tatccttcaa
    11881 gcgttttgcc aaattatcgc ttacaaaaat ttctttagaa tcagtcattc gaatcggcgg
    11941 tattcttata ctgtccccaa tttgtaaacg actgttagct tgtgctcttt ttttattaat
    12001 ccgaacctca ccgccacgaa taatcctgta gatatggctt ttaggaactc cttttaaaat
    12061 gcgaatcaga taattatcca aacgctggcc atcttcttcg gagccaattt ctttataact
    12121 tacttcactc attgtcaata aacctgtttt ctgagcgtga tttttttgat atgattagat
    12181 atcgtacggg aaaatccctt a
//
LOCUS       DAXLDT010000093        12104 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXLDT010000093 DAXLDT010000000
VERSION     DAXLDT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.093"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..3903)
                     /locus_tag="ACF2SP_002049"
     CDS             complement(<1..3903)
                     /locus_tag="ACF2SP_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFD2359826.1"
                     /translation="MIKSKTFSCYIIGEANITLQCADILLAGGHKLLGIISPSNTIKK
                     WCTANSIAYLENIKEFEKNHMDEEFDFLFSIVNSEIIPQKILRLPRYYAINYHNSPLP
                     KYAGLYATSWAILNGETQHGISWHIMNEVIDAGDILKQPTFPINDQDTAFSLNLKCYE
                     QGIYSFHELVEELSSHTTTLVKQNLSYRSYYGLKNKPANFGFISWEESSESIDRLCRA
                     LTFGNYTNQLEVPKIMINKEIYVVKSYEKLNISSGVKPGTIVNNSNGGLQVATGTTDI
                     LILELTDLEGRTCSMEQLGSLFKMTCYSQLDTIEPAFFEKLTICPAKKPKIEKFWVNE
                     FLKCSQEKNSFLSPLSQLDKTLCSLHDRKTVAQVPGELLAKLKKLCPEHWQIKNILLT
                     GLLIYLYRLNNYSNLSIAFINSQLSSNDPDLNKLLSDYVPLTTQFDSTMTFMDALTFV
                     SKEQGKLYENSTYTRDIFIRYPELHNAFNHIDISITFITDSKAVAPCVGNKKLNFCIA
                     EDCSWFYVNNKTNYRSHLESYAFFENMNEHLLTLLEDIVSNPDKKIFELSIISKKEKN
                     DLLVIWNNTQCDYDYEKLIHQYFEEQVSKTPEEIAAVFDNKSITYAELNRKANQLAHY
                     LRSQGVKQDDLVGISLSRSLEMVICILGILKSGGAYLPLDPNYPDERISYMLMDSKTN
                     LLITDQEFINRKPHGFNGKSIEINSFLNLKNLSSANLQAINKPSDLAYIIYTSGTTGK
                     PKGVAISHRSICNHMLWMKKEYAFKNKDVFLQKTPFSFDASVWEFFMPLLVGGKLVVA
                     PNDAHTSPNQMIRLIRENKVSVLQIVPSMLKELVSNEEFGLCKSLTHVFCGGEALLSE
                     TINAFFKYNFSDTKLHNLYGPTEVTIDTTAYTCTAGDAKGDVSRIGKPIMNTKVYVLD
                     IKMQPVPVGIMGELYISGDGLARGYLNNPEFTIQKFLPNPFSNNKNDRLYRTGDLVKW
                     DSNGVLEYHGRCDNQVKIRGYRIEINEIESYLEKIPSIHQCIVKPEKNQDDSMSLSAY
                     LVLEKNSQISAVDIRTILKQNIPEYMIPARFYIVDKFFSTPSGKIDRKILPIPSKRLR
                     SGTNYAPPNNSTEQLLHNIWCSVLKIDNLGIYDDFFELGGNSLSAMNIISHIREQFSL
                     TLSVRTLFDFPTISSLSKEIENIYHKKTDCIYNQPSGNNIIPLRTEGEKTPLFLVHPI
                     GGSVFWYKLLGQYFDKDRPLYGIQDPGLDRNELIFSNLEEMASAYVHSIQAIQPSGPY
                     ILGGASFGCTVAIEMAKQLEEKGETVISIISLDGWAFYP"
     gene            complement(4065..7217)
                     /locus_tag="ACF2SP_002050"
     CDS             complement(4065..7217)
                     /locus_tag="ACF2SP_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2359827.1"
                     /translation="MKIALFFIDRPVFATVISIIIVMVGVLSYFNLPIEQYPQVVPPT
                     IQVQASYPGANAKTVAETVATPIEQEVNGVENMLYMDSQSTDDGQMRLTITFKLGTDL
                     DQAQVLVQNRVAVVEPKLPEEVRRLGITTNKNSPDLMLVVNLYSPNGQYDQTYIGNYA
                     ILHIRDKIRRIEGVGNVLVFGASEYAMRIWLNPDLMNTFNLTANDVLAAVRAQNVQVA
                     SGKLNLQPQKNQYGFEYHIETKGRLVKKEEFENIIVKSGEGGRIVRLKDIGRVELGTQ
                     NYLTKGYLDKYPAVALPIYQRPGTNALATAQEIIKTMKTIAKDFPPGITYEIAYNPTL
                     FVQQSIDAVFHTIYEAIALVVLVILIFLQTWRASVIPVVAIPVSLIGTFAIMQAIGFS
                     LNYLTLFGLVLAIGIVVDDAIVVVENMERNIQAGMNPRDAARKTMTEVGSALVAMGLV
                     LIAVFLPTVFLEGISGRFYQQFGTTLAVATAISVFVSLTLTPTMAVLLLRAHHETIKQ
                     EGLAWWKKPIYGFLHGFNRLMEGFSRQYGKLVATLTRKTALMIIAYGIFISITLLLFV
                     YVPRGFIPRQDQGYFIVAVQLPPGASLSRTDAVINKAVKKILAIPGIAHTVSFTGFSG
                     ATFTNSSNAGAIFTPLLSFEERQRMGINYNDILKRLRQELSTIKEALIVVIPPPPVRG
                     IGNAGGFKMMLQDRGGRGLDVLMEAAATMVNAANQEKATTSVFTFFENSTPRLHLKLD
                     REKVERLNVPYANVVEALEVYLGSVFINEFNYLGRTFRVIAQADSEYRHTEDDILRIK
                     VKSNNGDMVPIGSVAQIENTVAPSRMPRFNLYPAIDLQGDVAPGYSSDEALATMEKLA
                     QNNLPDGIGYEWTEIAYQQKMVGNTALMAFTLGVIFIFLVLAAQYESWSLPLAVILIV
                     PMCLFSSMLGVKILGMENNIMTQIGFLVLIGLASKNAILIVEFARQLENRGYNLWKAA
                     IQAAKLRLRPILMTSFAFILGVFPLIIATGAGAEMRRALGVAVFSGMLGVTFFGLIFT
                     PLFYVLISRLSRYKRKLTRP"
     gene            complement(7317..8423)
                     /locus_tag="ACF2SP_002051"
     CDS             complement(7317..8423)
                     /locus_tag="ACF2SP_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2359828.1"
                     /translation="MKKILALVMRLLAVILIPVLMSCNSESNKPVGQTLAEVDVALPV
                     KKNIVEWDEYTGRFQAVEEVDVRARVTGYLEAIKFQDGQTVKKGDVLFVIDQRPFKYA
                     LARAEAQYALAKAQYERAVKLQKEKFISAEVIDQRFQDVQVAETRVQEAKLNLEFTEV
                     KAPISGKISRDFVSVGNLIRMNDTVLTKVVSVDPIYFYFETSQNDLLKYIRLDRAGKR
                     LSSEKKPTPVLIKLPDEKDYLHQGEVDFLDNVIDSGTGTILARALVPNPDNVIYPGLF
                     ARVKLVGSGEYEAILLPDKAINTDQARKFVYVVDNENKVNRVYVELGPLRESGFYIIR
                     SGLKGDEKVIIHGIQRIHGPNQEVKPVVIEVTEN"
     gene            8640..10478
                     /gene="legS2"
                     /locus_tag="ACF2SP_002052"
     CDS             8640..10478
                     /gene="legS2"
                     /locus_tag="ACF2SP_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector sphingosine 1-phosphate
                     lyase LegS2"
                     /protein_id="HFD2359829.1"
                     /translation="MFVRICAMFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYF
                     LYNQYHNPSISRWCRSRNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSSTREK
                     LRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDG
                     KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKE
                     GYGLLTHGGTTSIIEAMAAYVIRARAKGIDYPEIVVPETAHAAFKKAAELTGAILITV
                     PVDKKTGAVNPNVMSSYITRNTAVMVGSAPSFMNGIHDPISELGQLAKKKNVPFHVDA
                     CLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAA
                     LNWSGGLYATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELTA
                     LVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIADELEKRGWKLNLLQNPDGFHL
                     CLTHVHTLVGSFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPVELQKEIC
                     KQYQKARLDFTAASHGSLGIFAPSSTEEDDGLRNRKVGEQKVQTSL"
     gene            complement(10614..11375)
                     /locus_tag="ACF2SP_002053"
     CDS             complement(10614..11375)
                     /locus_tag="ACF2SP_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFD2359830.1"
                     /translation="MLKLFDAHFHIIDYRFPLVANQSYLPPEFTVEDYLQRAKPLNIY
                     GGAVVSGSFQAFDQTYLLTALSVLGPNFVGVTQLPSTVSDEEIIQLNQQGIRAVRFNL
                     KRGGSESIHQLKYFAHRIYEMVRWHVEIYVDSKELGDLTNLLLELPAVSIDHLGLSQS
                     GFSKLLHLVEHGIKIKASGFGRVDFDVKKALQTIAQINPDALLFGTDLPSTRAPRPFL
                     DSDIQLIRDLFDEELTEKILYRNACNFYSKSEPLT"
     gene            11552..11624
                     /locus_tag="ACF2SP_002054"
     tRNA            11552..11624
                     /locus_tag="ACF2SP_002054"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11585..11587,aa:Lys,seq:ctt)
     gene            11823..11978
                     /locus_tag="ACF2SP_002055"
     CDS             11823..11978
                     /locus_tag="ACF2SP_002055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359831.1"
                     /translation="MNDELRKIISDPQKKEDLKTGLSKHLSSKQTNKIKIGGKTYTVK
                     NINRSSR"
BASE COUNT         3670 a         2307 c         2338 g         3789 t
ORIGIN      
        1 agggtaaaat gcccaaccat caagggagat aatagaaatt actgtttctc ctttttcctc
       61 taattgtttc gccatttcaa tagcaacagt acatccaaat gatgcgccgc caagtatgta
      121 aggtccactt ggttgaatag cctgaattga atggacatat gcactagcca tttcttccag
      181 gttgctaaat atcaattcat ttctatcaag cccaggatcc tgtattccat acaatggtct
      241 gtctttatca aaatattgtc caagaagttt gtaccaaaaa acacttccac caattggatg
      301 tactaaaaac agaggtgttt tttctccttc tgttctaaga gggatgatat tatttccaga
      361 aggctgattg tatatacaat cagtcttctt atggtatatg ttctctattt ctttagataa
      421 agaggatatt gtgggaaaat caaataatgt tctaacgctc aatgtcagtg aaaattgttc
      481 ccgtatatgt gatattatgt tcattgctga tagtgaatta ccgccaagtt cgaaaaaatc
      541 atcgtaaatt cccaaattat ctatttttaa aaccgaacac cagatattgt gtaataattg
      601 ttctgtagaa ttattggggg gagcataatt agtacccgaa cgtaatcgtt tggatgggat
      661 tggtagtatt tttctatcta ttttaccgct tggggtgcta aaaaatttgt caacaatata
      721 aaatcgggcc gggatcatgt attcaggaat gttctgcttt aatatagtcc gaatatctac
      781 agcggatatt tgagaatttt tttccaaaac caaataagct gataaagaca ttgagtcgtc
      841 ctggtttttt tccggtttca caatgcattg atggattgaa ggaatttttt ccaaatagga
      901 ttcaatttca ttgatttcta tcctatatcc tctaattttt acttgattat cacatcggcc
      961 gtgatattcc agaactccgt tggaatccca tttgacaaga tcccctgttc tgtataatcg
     1021 atcattttta ttgttgctga atgggttggg taaaaatttc tgtatggtaa attcagggtt
     1081 gtttaaataa ccacgtgcta aaccatctcc tgaaatataa agctccccca ttattccaac
     1141 aggaacaggc tgcattttta tatctaatac atacactttt gtgttcatta tcggtttgcc
     1201 tattcggctt acatcgcctt ttgcatctcc tgcggtacat gtataagcag ttgtatcaat
     1261 ggttacttcc gttggtccat agagattatg tagttttgta tctgagaagt tgtacttaaa
     1321 aaaagcatta attgtttctg ataaaagggc ttcaccacca caaaatacat gcgtgagtga
     1381 tttgcagaga ccaaactctt cattagatac taattctttt agcatagaag gaacgatctg
     1441 caatacgcta actttgttct ctctgattag gcgtatcatt tgattgggac tggtatgtgc
     1501 atcatttggg gcaacgacca gtttaccacc aaccagaagt ggcataaaaa actcccaaac
     1561 agaggcatca aacgagaagg gggttttctg taaaaataca tccttgtttt taaaagcgta
     1621 ttccttcttc atccacaaca tgtgattgca aatagaacga tgcgatatgg ctacaccttt
     1681 aggttttcct gtagtcccag aagtataaat aatgtaggct aagtcggatg gtttgttaat
     1741 tgcttgaaga tttgcagaag ataggttttt taaatttaaa aaggaattta tctcaatact
     1801 tttgccattg aatccatgtg gttttctgtt tatgaattct tgatccgtta tcagcaggtt
     1861 agttttactg tccattagca tgtatgaaat ccgctcatca ggataattag gatctaatgg
     1921 caggtaagct cctccagact ttaaaatgcc taaaatgcat ataaccattt ccaagctgcg
     1981 acttaaagaa attcctacca ggtcatcttg ttttacacct tgagatctta aatagtgtgc
     2041 taattgattg gcttttcggt taagttctgc ataagttatt gatttgttat caaagacagc
     2101 agctatttct tcaggtgttt tggaaacttg ctcttcaaaa tattggtgaa tcagtttttc
     2161 ataatcgtaa tcacactgag tgttattcca gattaccaat aaatcgtttt tttccttttt
     2221 actgattatt gaaagttcga atatcttttt atcagggtta ctgactatat cttccaataa
     2281 ggtcagcaaa tgctcattca tattttcaaa aaaagcataa gattccaaat gggatctata
     2341 atttgtttta ttattaacat aaaaccagga acaatcctct gcaatacaga agtttaactt
     2401 tttattgccg acacaaggtg ccactgcttt tgagtcagta ataaaggtga tactgatatc
     2461 aatatggttg aaagcattat gtagctcagg gtagcgtatg aaaatatctc tagtgtaagt
     2521 gctgttttca tataacttgc cctgttcttt tgagacaaat gtcaatgcgt ccataaatgt
     2581 catagtgcta tcaaattgcg ttgttaaagg cacatagtct gataaaagct tatttaaatc
     2641 aggatcatta ctgctcaatt gtgaattaat gaatgcaatc gagagattac tgtaattatt
     2701 taatcgatat aggtaaatta agaggccagt taataagata ttctttattt gccaatgctc
     2761 agggcatagc tttttcaatt ttgcgagtaa ctcaccaggt acttgtgcta ctgtttttct
     2821 atcgtgaagt gaacataatg ttttatcgag ttgggatagt ggagataaaa atgaattttt
     2881 ttcttgagaa catttcaaaa actcattaac ccagaacttc tctatttttg gctttttagc
     2941 aggacaaatt gttagctttt caaaaaaagc aggttcaatt gtgtcaagtt gtgagtagca
     3001 cgtcatttta aataagctgc ctaattgttc cattgagcat gttctgcctt caagatcagt
     3061 aagctctaga atcaatatat ctgttgttcc ggtagctact tgtaagccac cattcgagtt
     3121 attcacaatg gtgcctggtt ttactcctga ggatatattt aatttttcat aagattttac
     3181 aacataaatt tccttattaa tcataatctt gggtacttct aactgattgg tataatttcc
     3241 aaatgttaaa gccctgcaga gccgatcaat actttctgaa gattcttccc atgaaataaa
     3301 tccaaaattt gccggcttgt tttttagacc ataataagag cggtatgaca gattttgttt
     3361 aaccaaagtc gtagtgtggg atgaaagctc ttcaacaagt tcatgaaagg aatagatccc
     3421 ttgttcataa catttcagat ttaaactaaa cgccgtatcc tgatcattta ttggaaaagt
     3481 tggttgcttt agaatatcac ctgcatctat cacttcattc ataatgtgcc aactaatacc
     3541 atgctgagtt tcgccattaa ggatcgccca cgaagtggca tataatcctg catatttggg
     3601 taatggggag ttatgatagt ttatggcgta ataacgaggg agtcttaaaa ttttttgagg
     3661 aatgatttca ctgttgacta tgctaaacaa gaaatcaaat tcttcgtcca tatgattttt
     3721 ttcaaattcc ttgatgttct cgagataagc aatggaattg gcagtacacc atttttttat
     3781 ggtgtttgat ggagagatta tgccaagtaa tttatggcct cctgctaata ggatatctgc
     3841 acattgaaga gttatattag cttcaccgat aatatagcaa ctaaatgtct tgcttttaat
     3901 catagtgctc catgctgtca gactggatcg attaataaaa gccatatttt aattgaaatg
     3961 atctttttca gtcaagtttg accaaaattt cactcatgac atcatgcaaa gaagagagga
     4021 accgagtgca taaataaatt aagggtttca cagcaaatat ttagctaagg tctggtcaat
     4081 ttccttttat atctactcaa gcgactaatt aaaacataaa acaaaggcgt aaagataagg
     4141 ccaaaaaaag tcactcccaa catcccactg aatacagcga caccgagcgc tcggcgcatt
     4201 tcagcgcctg cccctgttgc gataattaag ggaaaaacac caagaataaa tgcaaatgag
     4261 gtcatcaaaa tcgggcgcag acgaagcttt gctgcctgaa tggcggcttt ccagagatta
     4321 tagccccgat tttcaagttg tctcgcaaat tccacaatga ggatggcatt tttcgatgca
     4381 agtccaatca aaaccagaaa accaatctga gtcataatat tattttccat gccgagaatt
     4441 ttaactccca gcatggaaga aaagagacac atcggtacaa ttaatatgac cgctaagggt
     4501 aaagaccagc tttcatattg agcggcaagg acaaggaaaa tgaaaataac acccagagta
     4561 aaagccatca atgcagtatt acctaccatt ttctgttgat aggctatttc tgtccactca
     4621 taaccaatcc catctggaag attgttctga gccaattttt ccatggtcgc caacgcttca
     4681 tcagaactgt atccgggagc aacatcaccc tgtaaatcaa tagcgggata cagattgaaa
     4741 cgaggcatac gtgagggagc aaccgtattt tctatttgcg caaccgagcc tataggaacc
     4801 atgtctccgt tgttgctttt gactttgatt cgtaaaatat catcttcagt atgccgatat
     4861 tctgaatcag cttgcgcaat aacacgaaaa gtacgtccca ggtaattgaa ttcgttaata
     4921 aaaacagagc caaggtaaac ttccagtgct tccacgacat tggcgtaagg aacattaagt
     4981 cgttccacct tttctctgtc aagtttcaag tgaagtcgtg gtgttgaatt ttcaaaaaaa
     5041 gtaaatactg atgtggtggc tttttcctga ttcgctgcat taaccattgt tgctgccgcc
     5101 tccattaata catcaagacc tcgacccccc ctgtcttgaa gcatcatttt aaaaccaccc
     5161 gcatttccaa tacctcggac tgggggagga gggataacca cgatgagggc ttccttgata
     5221 gtacttaatt cctgccttag acgcttaaga atatcgttat aatttatccc catacgttgc
     5281 ctctcttcaa atgacaatag gggagtaaaa atggcaccgg cattcgatga attagtaaat
     5341 gttgcaccag aaaagccagt aaaactaaca gtatgtgcaa tgccaggaat cgccaatatt
     5401 tttttgaccg ctttgttgat gacagcatcg gtacggctca aagaagctcc gggcggcaat
     5461 tgaactgcaa cgataaaata gccttggtct tgtcttggga taaagccgcg tggcacgtaa
     5521 acaaatagaa gtagtgtgat ggaaataaaa ataccataag caataatcat taaagccgtt
     5581 ttacgtgtga gggtagcaac caatttccca tattgccggg aaaatccttc cattagtcgg
     5641 ttaaatccat gcaaaaatcc atatattggt tttttccacc aggctaaccc ttcttgtttt
     5701 atagtttcat gatgagcgcg aagcaataat accgccatgg ttggagtcag ggttaacgag
     5761 acaaaaacag aaatggcagt cgcgacagca agcgttgtac cgaattgctg ataaaaacgt
     5821 ccggaaattc cttccagaaa aacggtaggt aaaaatacgg caatcaacac caaacccata
     5881 gcgaccaatg ctgatcctac ttctgtcatg gtttttctgg cagcatcgcg gggattcatg
     5941 cctgcctgga tattacgctc catattttcg acaacgacaa tggcatcatc aaccacaata
     6001 ccgatagcaa gaacaagtcc aaacaaggtc aagtaattca acgaaaatcc aatagcttgc
     6061 attatggcaa aagttcctat caatgatacc ggaatagcaa ctactggaat gacagaagcc
     6121 ctccaggttt gtagaaaaat caaaatgacg agtacaacca gggcaatcgc ttcgtaaatg
     6181 gtatgaaata cagcatcaat cgactgttga acaaatagag taggattata ggcgatttca
     6241 taggtaatcc cgggtggaaa atccttggca atcgttttca tggttttaat gatttcttgt
     6301 gcagtggcta atgcattggt tcctgggcgt tgataaatag gtaatgcaac agcagggtat
     6361 ttgtctaaat aacctttcgt caggtaattt tgtgtaccta actcgacgcg accaatgtct
     6421 ttcaggcgaa cgatacgccc gccttcgcct gatttgacaa taatattctc aaattcctct
     6481 tttttaacca ggcgtccttt ggtttcaata tgatattcga aaccgtattg gtttttctgg
     6541 ggttgtaaat taagcttgcc gctggcgact tgcacgtttt gagcacgaac agctgctaaa
     6601 acatcatttg cggttaaatt aaaagtattc attaaatcgg gattcagcca aatacgcatg
     6661 gcatattcac tggccccaaa tacgagtaca ttgcctacac cttcaattcg tctgatttta
     6721 tcgcgtatat gcaagatagc gtaattgccg atgtaggttt gatcatattg accattaggg
     6781 gagtacaggt tcactaccaa catcaaatca ggtgaatttt tgttagtagt gattcctaag
     6841 cggcgtactt cttcaggtaa tttgggttcc acaactgcca ccctgttttg caccagtacc
     6901 tgcgcctgat ccagatcggt tccaagctta aaagtaatgg tcagacgcat ttgtccatca
     6961 tcagtggatt gggaatccat atacagcatg ttttctaccc cgttaacttc ctgttcaatg
     7021 ggggttgcca cggtttcagc tactgttttt gcattagctc caggataaga agcttgtacc
     7081 tgaatcgtag gaggaactac ttgcgggtat tgttctattg gtaagttgaa ataggacaat
     7141 acccccacca taacaatgat gatggagatt actgttgcga atacaggcct atcaataaag
     7201 aaaagagcta ttttcatcca gtaaatcctt tttgtgttgg ctacactgta aaacgttgta
     7261 tgctttatgc agtattaaat tctatcgaat gaacaggatt ccaacacaca actggtttaa
     7321 ttttcagtca cctcaattac cacaggctta acttcctggt ttgggccatg aatacgttgg
     7381 attccatgaa tgataacttt ttcatcccct ttcaatccac ttcggataat gtaaaacccg
     7441 ctttcgcgca gtggccctaa ctcaacatac actcggttca ctttattttc attatcaacc
     7501 acatacacaa atttgcgggc ttgatccgta ttgattgctt tatcggggag taaaattgct
     7561 tcatattccc cacttccaac taattttaca cgtgcaaaaa ggccaggata aatgacgtta
     7621 tcaggattag ggacaagtgc tctggcaagg atagttcctg tgcctgagtc aatcacatta
     7681 tctaaaaaat ctacttctcc ctgatgcaga taatcctttt catcaggtaa cttaattaag
     7741 actggagttg gttttttctc ggagctgagt ctttttccag cgcgatcgag gcgtatgtat
     7801 ttgagcaagt cattttgact ggtttcaaaa taaaaataaa tcggatcaac agaaactact
     7861 ttagtcaaga ctgtgtcatt catgcggatt aaattaccca cactcacaaa atcgcgactt
     7921 attttgccac tgatgggagc tttgacttca gtaaattcga gattcaattt tgcttcctgt
     7981 actctggttt cagcaacttg tacgtcttga aatcgttggt ctatcacctc agcggaaata
     8041 aatttctctt tttgtagttt tacggcacgt tcatattgcg ctttggctaa ggcatattga
     8101 gcttctgctc ttgccagagc atatttgaag gggcgttgat caataacgaa taaaacgtct
     8161 ccttttttta ccgtttggcc gtcctggaat ttgatcgcct caagatatcc agtgacacgg
     8221 gcacgaacat ccacttcttc tactgcttga aatcgtccag tatattcgtc ccattctaca
     8281 atattctttt ttactggtag cgcgacgtca acttctgcca aggtttgtcc cactggctta
     8341 ttggattctg aattacagga catgaggaca ggaataagaa taacagccag taatcgcatg
     8401 accaatgcca gtattttttt cattgttatc tatccctgat attcaattca ctggtaagtt
     8461 atgatccagt agataagctt agtttagctt ataaagatgt attatgcgta atgccgtgaa
     8521 tattatatct tgtatttgga gattatccgg gtcaagcaaa ataccagcga atttttgtta
     8581 tactggagcg catcaaccgg tctttatttt tttatgcaaa gtgactatta tccaacattt
     8641 tgttcgtaag gatatgtgca atgttcggtt ttatttcaga tttattaaca gcagcagtca
     8701 gttctttaga tgaattatta caagatactc cggcacatca aatcatatta ggcacagcgg
     8761 ctctttattt tctttataat caataccata acccatcgat ttccaggtgg tgccgtagcc
     8821 gcaataacgc cagtatgaag cagcgtatta ttgatagtgc ttacgccttg gcaaaaaatt
     8881 tacctggtgt gaatcagatc attgagaaag agctcaataa agaactctct tcaacgagag
     8941 aaaaattgag aattcagcgt tccggtatga ctctcaggga ggaaatacct gaggaagggc
     9001 tttctcccca agatattttg agtgcatttg atgtggatgt ggaaaaatgt cattttgatt
     9061 ttttatccgt aactaatgac agccctgaga gggagtttct tgttggaaga ggggatggta
     9121 aagattcagg tgcattatac gctattcacc ctaaggaatt gacagagctg cttaaagaag
     9181 tttatggggc aaccgctcta actaatcctt tgcatgataa atggcctcga attaacgcaa
     9241 tgcaagcgga ggtgattcgc tggtgtcaaa atttgtttca tggttcaaaa gaaggttatg
     9301 gtttattaac tcatgggggt acgaccagca ttattgaggc gatggctgct tatgtcattc
     9361 gcgcaagggc aaaaggcatt gattatcctg aaattgtcgt tcctgaaaca gcacatgctg
     9421 catttaaaaa agcagccgag ttaactggag caatattaat tacggttcct gttgataaaa
     9481 aaacgggtgc ggtgaatccc aatgtcatga gttcctatat taccagaaac actgctgtaa
     9541 tggttggatc tgctccatcg ttcatgaatg gaattcatga tcctatctct gagctaggcc
     9601 aattagcaaa gaaaaaaaat gttccttttc atgtagatgc ctgtttgggt ggatttttaa
     9661 cggctttttt agacacttca tccgagccta tggattttag agtgcctgga gtgacttcca
     9721 tttcagccga cttgcataaa tatggctgtt gtccaaaagg cacctctgtt tgtcttttta
     9781 gtgaggattc gcctgctttg tcggtttatg ccgcattaaa ctggtcaggt ggattgtatg
     9841 ctacgccagg aattctggat ggttcaacca gtggagctcg tgtcgcggaa gtctatgcta
     9901 cattgtctta ctatgggaaa aataaatatc aggaaatcgc caaaagcatt atcagattac
     9961 gcaatgctat tcaaaaagaa ttgacggcat tagtggaaga aggtaatgga ttaaccagcg
    10021 aggatattta tgtttatggt aatcctcaat ggtccattct gggttttaga agtaacactt
    10081 gcaatgccca tttcattgcc gatgaattgg agaaacgggg gtggaagctt aacttgctac
    10141 aaaatccgga tgggtttcat ttatgtttga cccatgtgca cactttagta ggaagcttcg
    10201 agacgcaatt tatcaaagat ttgagagagg cggtgattga tgttaaaaat tatccccctg
    10261 gaaagaaacc aagcggtaat gtcaaagtgt atggtgctgt cggtatgatg ccggttgaac
    10321 ttcagaaaga aatttgtaaa caatatcaaa aagcccgcct tgattttaca gcagccagcc
    10381 acgggagctt ggggatattt gcaccttcat ctacagaaga ggacgatggg cttagaaaca
    10441 ggaaagtagg ggaacaaaaa gtgcaaacct cgctttgaac caatagagct cttgcataac
    10501 cagtctttat ctttttaatg ctgccttgca aacgtttctg cggcgctgat ttactgcatc
    10561 taaattaagc tcgctgagac gtttgcgcct tgccgtaaaa caaaataatc agcttatgta
    10621 agaggttcgc ttttgctata aaaattacac gcgtttctgt acagaatttt ttctgtcagc
    10681 tcctcatcaa atagatcccg gattaattga atgtcactat ccagaaaagg gcggggagcc
    10741 cgcgtggagg gtaaatctgt accaaataac aaagcgtctg gattaatttg ggcaatggtt
    10801 tgcagtgctt ttttaacatc aaaatcgact ctcccaaaac ctgaagcttt tattttgatg
    10861 ccatgctcca ccagatggag taacttggaa aatcctgact gtgataatcc taaatgatca
    10921 atactgacag ctggcaattc caataacaga ttagtcaaat caccaagctc ctttgagtct
    10981 acataaatct ctacatgcca ccttaccatt tcataaatac gatgagcaaa atactttaac
    11041 tgatggatgc tttctgaacc tccacgcttg agattgaaac gaaccgctcg aatgccttgt
    11101 tgatttaatt gaataatttc ttcatcactt acagtggaag gtaattgagt aacccctaca
    11161 aagtttgggc ctaaaaccga taaagccgtt agcagatagg tttggtcaaa ggcttgaaat
    11221 gaacctgaaa ccacggctcc tccataaata ttcaagggct ttgcacgttg gagataatct
    11281 tcaacagtaa attcgggagg aagataggat tgattagcga ctagaggaaa acgataatca
    11341 ataatatgaa aatgagcatc aaatagtttc agcatttcag tcaattaaat ttgcgattaa
    11401 aatcatacta tcatacaaag gtttcattcg ttattagcga ttggtttttt gcttgatgac
    11461 tataaatgat ataaggccat tctctaattt atttgtcagt tgacagatag aatacttgtt
    11521 gctattaaat tgtaagtggt ttttataata tgggccgtta gctcagttgg tagagcagga
    11581 gactcttaat ctcagggtca taggttcgaa tcctatacgg cccaatcaat aaaatcaatg
    11641 agttatataa attagttact accaaaatat ttaattgatt atctatggga caatgatggg
    11701 acattgagaa atagcaatct atcacaatac atatctttaa attcttttct taaacatata
    11761 acatttattg ctataattta gatataaaat atcgatttac gaggttacgt atgaaagtca
    11821 caatgaatga tgagttacgt aaaataatca gcgatcccca aaaaaaagaa gatttaaaaa
    11881 ctggtctatc aaaacatctt agtagtaaac aaactaataa gataaagatt ggtggaaaaa
    11941 catatacagt gaaaaatatt aaccgcagtt ctcggtaact tcccaataaa caaacatgaa
    12001 tattgcattt ccgacattac taatttttct tcttctttta ccaggcttta tttttacact
    12061 cgctttttat aaaactgaag agcctcttag ttatctacct ctca
//
LOCUS       DAXLDT010000094        12088 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXLDT010000094 DAXLDT010000000
VERSION     DAXLDT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.094"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            133..1899
                     /locus_tag="ACF2SP_002056"
     CDS             133..1899
                     /locus_tag="ACF2SP_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFD2359832.1"
                     /translation="MTSLQIISHFFPFFWPKNNFKKKFRVIFSLFLVFTTLALNLIVP
                     IFFKDIVTALSGTIRTSSMMIISLICCYGLLWMLARCSEKIREMLSFVNISTTIADYS
                     THVFAHLHALNYKFHLDKETGKITSAISRAQLAIAMLISNILFRIVPVFLEILFAFLI
                     IWHFYGLSYGLYLIIILGIYLVFNGLTQKKSTVLQKNCSIAELNVSSAITDSLLNTET
                     VKYYNSQTVEHKKIDQFLQESVHANTALFSGNGTFLVIQTLIIAGGLAFLSYKVGYQI
                     LIGQLKIGDFVLINGYLLQLFEPLSEASVRWRDTKNDLSKIEYSAYLLDQTDEVEQDH
                     PDAKPLFITGPHVHFKNVTFGYQPEHPILKNVSFEVPPAKMTAIVGPSGSGKSTIARL
                     LLGLFEVTQGQILIDDQDITAVSKHSLRQQIGIVPQEVLLFNNTLRFNLCYGALNISE
                     REINNVIQLAHLEEMVKNLPKGIETNIGEGGFKLSGGERQRIGIARCLLRNPSILLFD
                     EATASLDTQTEKNIQENIEEVTKHTTSIVIAHRLSTIIHADNILVLQDGEIVEAGTHQ
                     ELIEKKGLYYSMWNSQQQKSAS"
     gene            1896..3704
                     /locus_tag="ACF2SP_002057"
     CDS             1896..3704
                     /locus_tag="ACF2SP_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFD2359833.1"
                     /translation="MMNESLPSIKETIYYLWPCVWTKNSLRGKIHVVLALLFILISIA
                     LNLCVPIFLKEAINALSGHALFLNSTPLVIILTYASVWGASKACITLSQVIAFPVELE
                     GARKFCLELFDHVQALSTKFHRERKSGEILTIIERSHSSAVDLIGRPIVMILPVLIEI
                     IFAMIFLSYFYDLFFGLVLLLMLVSYILLSYYTAQWIVKCRMKQNNEDASANAYLVES
                     LLHADTVKYFNTQQDEINTAAQKLQEKACADTRALNADAKIHLMQNVIVGMCVIIMLL
                     LAGSRVIAGSMNVGDFVLVHGYLFMFMLPLSMLGYRIRVTRDDLARFQTAIQLLKIPI
                     DIQDQPNARTLKYKEGKIQFDHVHFTYNEHRKILDDVSFIVEPGTTTAIVGSSGSGKS
                     TLARLIFRLYDLNSGSIQIDGQNISNIQHASLRAILGIVPQETALFNDTLRNNLIYGN
                     PHVTHDELADAIQAVHLDRFVAKLPDGLETRVGERGLKLSGGEKQRVAIARMLLKKPK
                     IFIFDEATSSLDMKTEKDIQTCLKQISINTTTLIIAHRLSTVTHADNILVLAKGVIIE
                     QGTHNELLNQNGAYAQLWKAQSQNKIDTGSINGFVP"
     gene            3786..4628
                     /locus_tag="ACF2SP_002058"
     CDS             3786..4628
                     /locus_tag="ACF2SP_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HFD2359834.1"
                     /translation="MNIKQNDTSTTPPILYGTAWKEDDTKRLVLQALNSGFRGIDTAN
                     QRRHYFEEAVGDAIDQFLTTSQKTRSDLFLQTKFTSVHGQDHRKPYDEFDSLTNQVKQ
                     SFASSLEHLQTDYIDAYILHGPSLSHGIIDADLEIWQAMEELVCSRKVKFLGISNVNM
                     VQVEELYRKVSIKPSFIQNRCFAITQWDQDVRTFSQKNKIIYQGFSLLTANQRYLLNP
                     HMQSLAVKYDKTIPQIIFRFANQVGILPLTGTTNQQHMDNDLNIYDFELTQEEIQYIE
                     NIGL"
     gene            4639..5139
                     /locus_tag="ACF2SP_002059"
     CDS             4639..5139
                     /locus_tag="ACF2SP_002059"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2359835.1"
                     /translation="MTIKIETPRMLLRLIKDEDLDQVAQLHADREVRKFFPDGTQDRE
                     QTKRRIKQIMNLYGDKGLPGFVIFNKVTHEFIGRCGFGPIETGEIEVGYLIVRKFWGM
                     GYASEALEALLEWSKININSDYIIAFAPLKHSASHRVMEKCGMEYYKDELGHGVECKF
                     YRSKNK"
     gene            5162..5944
                     /locus_tag="ACF2SP_002060"
     CDS             5162..5944
                     /locus_tag="ACF2SP_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFD2359836.1"
                     /translation="MTKITTDEAALKLAVLIDADNARAVIIDGLLTEIAGYGDAIVRR
                     IYGDFTLPSSAQWKKVLHKYAIKPVQQFAFTSGKNATDSALIIDAMDLLYTHRFGGFC
                     IVSSDSDFTSLANRIREEGLQVFGFGEKKTPEAFRNACNKFIFTEVLRPVAPPDQLIS
                     QSKKRKQPVKQAKANEPSAQEFPKEFILEALNNSYDDTGWAYIGTFGGYLTKLKPDFD
                     SRLYGFKKLSDLVKGKPEIFETEERVNPGSNTKVLYLRGRQE"
     gene            5959..6336
                     /locus_tag="ACF2SP_002061"
     CDS             5959..6336
                     /locus_tag="ACF2SP_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HFD2359837.1"
                     /translation="MNLLEKIAALENEATEFGFQWETTDQIMTQIQSECIEVDEHLKA
                     GINDNSSALQEEIGDLLHAVFSLCVFCKLDLKDTLQQTVEKFERRLRAVKMIAHEMKL
                     INLEGQTFDELMSIWKQAKSRVG"
     gene            6642..8093
                     /locus_tag="ACF2SP_002062"
     CDS             6642..8093
                     /locus_tag="ACF2SP_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn5 family transposase"
                     /protein_id="HFD2359838.1"
                     /translation="MIKGNQNNSSSGWAESEFGIVNFGDKRLSKRLLKIADSFANSPE
                     CSINRACDDWHQSKAAYRFFQNDAVSERKILDSHITKTIERAKNYPTILAIQDTSYIS
                     YKNHKKTEGLGIIAARVRSKTTNFQTHGLVMHTTFAVTTEGLPIGLLDQKISSRPLLD
                     ETVKELKKRSHNIALPIEEKESMRWIESLEHSNNYPDLKNAKVVTVCDREADIYDLFE
                     VASTNQCPFVVRARQDRTVNKTSIYSKKSGEKLWDLVSGSPCRGEIQVTIPARDNKPK
                     RTATLEVRFDHFVMNPPKNNVKRKTRTLPDLKLNAVYVIEQSPPLGEEPMNWMLLTNI
                     DINNFEEAVEKIQWYCLRWRIEIFHKILKSGFKVEECRLGAADRLVRFLTIMSVIAWR
                     IFFITLVARESPDLPCSFLLANEEWNVLYTKIHHTKHYPETPPTIKEAVKWIAQLGGF
                     LARKNDGEPGPITLWRGWKRLVDLAEGWNLALS"
     gene            complement(8164..9684)
                     /gene="ltrA"
                     /locus_tag="ACF2SP_002063"
     CDS             complement(8164..9684)
                     /gene="ltrA"
                     /locus_tag="ACF2SP_002063"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFD2359839.1"
                     /translation="MTDNLLSGAPDAFILNTKWDQLDWPSIEKNVYRLQVRIAKAVSN
                     KQHGKVKSLQWLLVNSISAKLLAVRRVTTAKGSKTPGIDGVVWTTSEEKCEAVRNLKA
                     RGYKATPLRRIYIPKKNGKERPLSIPTLKDRAMQALYLLALEPVGETTADLNSYGFRP
                     KRSTHDAIYQCYATLARKNCAQWILEGDIKACFDEIDHGWLKSNIIIDQRVLTQWLQA
                     GYMEKNQLFETARGTPQGGPASPLLANMVLDGLEREIHSGCGQGNKINYIRFADDFIV
                     TANSPDILKEKVMPIISNFLAQRGLSLSQEKTKIVHIEEGFDFLGFNVRKYKGKFLTT
                     PSKDSIKSVQMKIKETVKKGYGWKGSELISALNPIIKGWANYHRKVVSKATFSELDNY
                     IYQETFRWTMRKFSGHNRYKAMDRYFRNRSLTRRWIFSDVVKAKDGTKKYVCINKMMD
                     IKIQRHVKIRSSANPYLPEYVKYFEDRKKWAKDCSFVQRIKDKQLNVIGSEYLLGE"
     gene            10577..10906
                     /locus_tag="ACF2SP_002064"
     CDS             10577..10906
                     /locus_tag="ACF2SP_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359840.1"
                     /translation="MARITVTLPDNLHKQIIKIAGKENDSLSYTTTRLVEIGLMVMNS
                     KSDNKDEQKTTNIEEYCQKLIIQINGIIKEIAVDKFNFDDDKIVQITKDTLNKFNKLK
                     GIQQESL"
     gene            complement(11104..>12088)
                     /locus_tag="ACF2SP_002065"
     CDS             complement(11104..>12088)
                     /locus_tag="ACF2SP_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359841.1"
                     /translation="SSLQTSRIKRRIYEYEDIPRFKGAEVIQTVYSIEQIEKGDPKKY
                     ENFIITDFKNSKIVEWSCAPEMLDNYYKDTGKPFQTSPAFFNPEVLSKYKNNHEKYEV
                     RDRGIHCYGSWSLKSYSINDEGQVHAYIYCLGQLPHKEQLHWKQYNEEPKAGISKVAY
                     NTDFLAEFDEEESSLRAIKQKLANFPPYSLDKEKFVLWAPKGGDIDMLLKQIHPVITD
                     IKKEYLDYLLNLTILVIDGLQEKVIRRLVNAEENKRSLECLEILLKNWGSPNADKIMK
                     ALRCLQAKRSKYAGHGGGKIDFDIKEDSLAITYEINEAIHLLSEEIDKNSR"
BASE COUNT         3841 a         2295 c         2227 g         3725 t
ORIGIN      
        1 tattccacaa atgtatgttt ttttgaacac aaattatttt gcgcttgcct caatgtctac
       61 ggttttttta cttcctaaat tatttaatgt tatcctcctc aatcatatga taataatcaa
      121 taaggactct atttgacgag tttacagata atatcccatt tttttccatt tttttggccg
      181 aaaaataatt ttaaaaaaaa gtttcgggtc atattttcat tatttttggt tttcacaaca
      241 ctcgccttaa atttaattgt acccattttt tttaaagata tagtaactgc attatccggt
      301 acgataagga catcgagcat gatgattatc tcgctgatct gctgttatgg attactctgg
      361 atgctggcaa gatgcagtga aaaaattcgc gagatgcttt cttttgtaaa cataagtaca
      421 acaattgcgg attatagtac tcatgtcttt gcacaccttc atgctttaaa ttacaaattt
      481 catctggata aggaaaccgg aaaaataacc agcgctatca gtcgcgcaca gcttgcaatc
      541 gcgatgctta tctcgaacat attgtttcgg attgtgcctg tttttttaga aattctcttc
      601 gcttttttaa tcatttggca cttctatggc ttaagctatg gcctctatct aattattatt
      661 ttgggaatct atcttgtttt taatggactc actcaaaaaa aatccacagt cctgcaaaaa
      721 aattgtagca ttgctgaatt aaatgtaagt tccgcaatta ccgacagcct actcaacaca
      781 gaaactgtaa aatactacaa cagtcaaacc gtagaacata aaaaaataga ccaattttta
      841 caagaaagcg ttcatgcaaa tacagcatta tttagtggaa acggtacatt tttggtgata
      901 caaacactca ttattgcggg gggtcttgcc tttttgtcct ataaagtagg gtaccaaatt
      961 ttaattggac agttaaaaat tggtgacttt gtcctgatta atggctattt attacagtta
     1021 tttgaacctt taagcgaagc tagtgtgcgt tggcgagata ccaaaaatga tttatccaaa
     1081 atagaatatt cagcctattt attagatcaa accgatgaag ttgaacaaga ccatcctgat
     1141 gcaaaacctt tattcattac cggcccgcat gttcacttta aaaacgtcac tttcggttac
     1201 caaccagaac accccatttt aaaaaatgta agcttcgaag ttccacccgc aaagatgaca
     1261 gctattgtgg gacccagcgg atcgggtaaa tcaacgattg cacgattatt gctgggatta
     1321 tttgaagtca ctcagggaca aatactcata gatgaccagg atattactgc tgtgagcaag
     1381 cattcattac gccaacaaat cggaattgtt ccacaagaag ttctactctt caacaatacg
     1441 ttgaggttta atctctgcta cggtgctcta aatatctccg agcgtgaaat caataacgta
     1501 atccaactgg cgcacttgga agagatggta aaaaacctcc ccaaaggaat tgaaaccaac
     1561 atcggtgaag ggggttttaa attatcagga ggagaacgcc agaggattgg aatagcaaga
     1621 tgtttattac gcaacccgag tattttgtta tttgatgaag caaccgcatc gctcgacacg
     1681 cagactgaaa aaaatatcca ggaaaatatt gaggaagtta caaaacatac caccagtata
     1741 gtcattgcac atcgtttgag tacgattatt catgctgaca acattcttgt tttacaagac
     1801 ggagaaatcg tagaagctgg aacgcatcaa gaattaatcg agaaaaaggg attatattac
     1861 tccatgtgga atagccagca gcaaaagagt gcatcatgat gaatgaaagt ttacccagca
     1921 ttaaagaaac tatctactat ctttggcctt gcgtttggac aaagaacagc ctaagaggaa
     1981 aaattcacgt tgtcttggct ctcttattta tattgatttc cattgcacta aatttgtgtg
     2041 tccccatttt tttaaaagaa gccattaatg cactctctgg acatgcccta tttttaaaca
     2101 gcaccccttt agtcatcatt ctgacttatg catcggtttg gggtgcatcc aaagcttgta
     2161 tcaccctcag tcaagtaata gcttttcctg tagaattaga aggggcgaga aaattttgtt
     2221 tagaactttt tgatcacgta caagcgcttt ccacaaaatt tcatagagaa cgtaaatcag
     2281 gcgaaattct tacaatcata gaacgttcac actcgtctgc tgttgatctt attggtcgac
     2341 ctattgtgat gatattgccg gttcttatag aaataatttt tgctatgatt tttttgagct
     2401 atttttatga tctttttttt ggattggttc tattgttgat gctggtttct tatatcttgc
     2461 taagctatta caccgctcaa tggattgtaa aatgcagaat gaagcaaaac aatgaagatg
     2521 catctgcaaa cgcctatctg gtggaaagcc tgttgcatgc ggataccgtg aaatatttta
     2581 atacccaaca ggatgaaatc aatacagctg ctcaaaaatt acaagaaaag gcctgtgcag
     2641 atactcgagc attaaatgcc gatgcaaaaa ttcatcttat gcaaaatgtc attgtcggta
     2701 tgtgtgttat tattatgctc ttacttgctg gcagccgagt cattgcaggg agcatgaatg
     2761 tcggagattt tgtactggtc catggctatt tattcatgtt catgctgcca ttatccatgc
     2821 taggctatcg aattcgtgtt acccgagatg atttagctcg ttttcaaacc gcgattcagt
     2881 tgctgaaaat tcctatcgat attcaagacc aacccaatgc cagaacactt aagtacaaag
     2941 aaggaaaaat ccaatttgat cacgtccatt ttacttataa tgaacatcga aaaattcttg
     3001 atgacgttag ctttatagtt gaacccggta ccactactgc aatcgttgga tcctcaggat
     3061 ccggcaaatc cactctcgca agattaatct ttcggcttta cgatttgaat tcggggagta
     3121 ttcagattga tgggcaaaat attagcaata tccaacatgc atcactcaga gcaatactcg
     3181 gtattgttcc tcaggaaact gctttattca atgatacctt aagaaataac ctgatatatg
     3241 gaaatccgca tgtcactcat gatgaattgg cggatgcaat acaagcagta catcttgaca
     3301 ggttcgtggc aaaactgcct gatgggttag aaaccagagt tggtgagcgc ggattaaaat
     3361 tgtctggtgg cgaaaaacag cgagtggcca tcgctagaat gctgcttaaa aaacctaaaa
     3421 tttttatttt tgatgaagca acatcttcac tggatatgaa aacagagaaa gacattcaga
     3481 cctgtctcaa acagatatcg atcaacacaa caacacttat tattgcacat agactttcta
     3541 cagtaacgca tgcagataat attctggtac tcgctaaagg tgtgattatt gagcaaggta
     3601 cccacaatga actactaaat caaaatggtg cctatgctca attgtggaaa gctcaaagtc
     3661 aaaataaaat tgacacaggg tctattaatg gctttgttcc ttagctcatt atcctccgaa
     3721 aaatacctct atattttgag gaggataatg cttggttatg ctgtgacacc aatggaggcg
     3781 gcattatgaa tataaaacaa aacgacacat caaccactcc ccctatttta tatggtacag
     3841 catggaaaga agatgacaca aaacgtttgg tgctccaggc actcaactca ggatttcgag
     3901 ggattgatac tgccaatcaa cgtaggcatt attttgaaga agccgtgggt gatgcaattg
     3961 atcaattttt aacaactagt caaaaaacac gcagtgattt atttctacaa acaaaattta
     4021 cctctgtaca tggccaagat catagaaaac cttatgatga atttgattca ttgaccaacc
     4081 aggtcaagca atcctttgca agctcactag aacatttaca gacagactac attgacgcct
     4141 atatacttca tggcccttca ctatctcatg gaataatcga tgcagattta gaaatttggc
     4201 aggccatgga agagctggtg tgtagtagaa aggtaaagtt tctcggcatt tctaacgtta
     4261 atatggtaca agttgaagaa ctatatagaa aagtctctat aaagccgtcg tttatacaaa
     4321 atagatgttt cgccataaca caatgggatc aagacgttag gacatttagt caaaaaaaca
     4381 agattatcta tcaaggattt tctttgctca ctgcgaatca gcggtattta ttaaaccctc
     4441 acatgcaatc acttgctgta aaatatgata aaaccatccc gcaaattata tttcgctttg
     4501 caaaccaagt ggggatattg cctttaaccg gcactacaaa tcaacaacac atggataatg
     4561 atttaaacat atatgacttt gagcttacac aagaagaaat tcaatatata gaaaatatag
     4621 gattataagt gaaccaatat gaccattaaa attgaaacac caaggatgct tttgcgttta
     4681 attaaggatg aggacttaga tcaagttgct cagcttcatg cagaccgaga agttagaaaa
     4741 ttctttcctg atggaacaca agatcgcgag cagactaagc gaagaataaa acagattatg
     4801 aacctttatg gagataaagg attgcctggt tttgttatat tcaataaggt gactcatgaa
     4861 ttcattggac gatgtggatt tgggccaatt gaaacagggg aaattgaagt tggatacctt
     4921 attgtaagaa aattttgggg tatgggttat gcatctgaag cactagaggc acttcttgaa
     4981 tggagtaaaa taaatatcaa ttctgattat attattgctt ttgcgccact caaacatagt
     5041 gcatcacatc gagtaatgga gaagtgtggt atggaatatt ataaagatga acttggtcat
     5101 ggtgttgagt gtaaatttta tcgttccaaa aataaataat ttataaaaca ggtcaattag
     5161 catgacaaaa attacaacag atgaagccgc gttaaaatta gctgtactta ttgatgctga
     5221 taatgctcga gcagttatta ttgatggtct tttaactgag attgctggtt atggagatgc
     5281 tatcgtaagg agaatttatg gcgatttcac cttaccatct agcgcacaat ggaaaaaggt
     5341 attgcataaa tacgctataa aaccagtaca gcagtttgct tttacttctg gaaaaaatgc
     5401 aacagatagt gcacttatta ttgatgctat ggatctactt tatacgcatc gttttggtgg
     5461 tttttgtatc gtgtcaagtg atagtgattt tacaagtctt gctaaccgta ttcgtgaaga
     5521 agggttgcaa gtttttggat ttggagaaaa aaaaacacca gaggcatttc gtaatgcctg
     5581 caataaattt attttcactg aagtactacg ccctgtagca ccacctgatc aattaattag
     5641 ccagtcaaaa aagagaaagc agcctgttaa acaagcaaaa gctaatgaac cctcagcaca
     5701 agaatttcca aaagaattta ttctagaagc attaaataac tcatacgatg atactggttg
     5761 ggcatacata ggaacatttg gtggctattt aactaagtta aaaccagact ttgattccag
     5821 gctttatggt ttcaaaaagc tgagtgattt ggttaaggga aaaccggaga tttttgaaac
     5881 ggaagaacga gtcaatcctg gatcgaacac aaaagtttta tatttacgtg gtagacaaga
     5941 ataacgtgga aataattaat gaatctatta gaaaaaattg cagcattaga aaatgaagct
     6001 actgaatttg gttttcagtg ggaaacgacc gatcagatta tgactcaaat ccaaagtgag
     6061 tgtattgaag tcgatgagca tttaaaagca ggaattaatg ataactcatc tgctttgcag
     6121 gaagaaatag gtgacctatt gcatgccgtg ttttcactgt gtgtattttg taaattagat
     6181 ctcaaagata cactgcaaca aaccgtggaa aaatttgagc ggcggctccg cgctgtcaaa
     6241 atgattgctc atgaaatgaa actcattaat ttggaaggac aaacttttga tgagttgatg
     6301 tctatctgga aacaagcaaa gagcagagta ggttaacgct gggtgagcat gacttgtaaa
     6361 ttgggtagcc gaaatgtaga ttcagcataa aatcacaaac gcgcaattgt cctgcgcttg
     6421 ataaaatatt ttcatgctcg cataaatttc cgtacaaaat taatgaataa aatcaaccta
     6481 taaaaagaag acaggtcggg taacaggatc gagttgcccc gatcccgtcc cctaagaacc
     6541 ttactattac ccataagtct ataaattata agaaaaactt tttctttaat ttaactctct
     6601 gttataaata agggcagtat tttgactgat aatgagagca aatgataaaa gggaatcaaa
     6661 ataactcatc ttctggatgg gcagagagtg agttcggaat agttaatttt ggtgataaac
     6721 gattaagtaa aagacttcta aaaatagcag atagctttgc caactcccct gaatgttcaa
     6781 taaatcgagc atgtgacgat tggcatcaaa gtaaagcagc ttatagattt tttcaaaatg
     6841 atgctgtatc ggaaagaaaa atccttgata gtcatatcac taaaactatt gaaagagcca
     6901 agaactatcc aactatttta gccatccagg atacgagtta tatttcttat aaaaatcata
     6961 aaaagactga aggattagga attatagcag ctagagttcg ctctaaaaca actaattttc
     7021 aaactcatgg attggtaatg cataccacat ttgcagtaac tacagaaggc ctgcctatag
     7081 gattactgga tcaaaaaatt agttctagac cgcttttaga tgagacagta aaggagttga
     7141 aaaaaagaag tcataatatc gctcttccta tagaggaaaa agaaagtatg cgttggatcg
     7201 agtctctcga acactctaat aattatccgg atttaaaaaa tgctaaggtg gttactgttt
     7261 gtgatagaga ggcagatatt tatgatttat ttgaagtagc atcgacaaat caatgtcctt
     7321 ttgtagtgag ggcacgtcaa gatagaacag taaataagac atcaatttac tctaaaaaaa
     7381 gtggtgaaaa attatgggat ttggtgagtg gttcgccttg tcggggggaa attcaagtca
     7441 ctattcctgc tcgagataat aagcctaaaa gaacagcaac attagaagta aggtttgatc
     7501 attttgtgat gaaccctcct aaaaataacg tgaaacgtaa aaccagaaca ctccctgatt
     7561 taaaactaaa tgcagtgtat gtcatagaac aatctcctcc tctgggcgaa gagcctatga
     7621 attggatgtt gttgaccaac atagacatta ataattttga agaggcagta gaaaaaatac
     7681 aatggtattg cttaagatgg aggatagaaa tttttcacaa aatcttaaaa tctggtttta
     7741 aagttgaaga atgcaggctc ggtgcagccg atagattagt tcgctttctt acaataatga
     7801 gtgtcattgc atggcgaatt ttttttatca cactagttgc tagagaaagc cccgatcttc
     7861 cttgttcgtt cttattggct aatgaggaat ggaacgtctt gtataccaaa atacatcata
     7921 caaaacacta tcctgaaaca ccacccacaa taaaagaagc cgtgaaatgg atagctcagc
     7981 taggcggctt tttagcccgt aaaaacgatg gggaacctgg tcctataaca ttgtggagag
     8041 gatggaaaag attagtcgac cttgcggaag gttggaatct tgctctttct tagttatggg
     8101 taatagtaag cctaagaacg cagcatgcga ctttcaccgc actacgctca agcctttctg
     8161 aagctattca cccagcaggt attcgctacc aataacattc aattgtttgt cctttattcg
     8221 ttgaacaaag gagcagtctt tagcccattt ttttctatct tcaaagtatt taacatactc
     8281 gggtagatag gggttggcgc tcgacctaat tttgacgtgt cgttgtattt ttatatccat
     8341 cattttgtta atacaaacat acttcttcgt accgtccttg gccttaacca cgtcactaaa
     8401 aatccagcgc cttgtaaggc tacgatttcg aaaataacga tccattgctt tatagcgatt
     8461 atgaccacta aacttcctca tcgtccaacg gaatgtttct tgatatatgt aattatccag
     8521 ttcggaaaat gttgcttttg agacgacttt tcgatgatag ttagcccatc cttttatgat
     8581 aggattcagc gctgagataa gctccgagcc tttccaacca tatcctttct tgacggtctc
     8641 tttgattttc atttgtacgg atttaatgct gtcttttgag ggcgtagtta agaacttacc
     8701 cttatatttc ctgacattaa atccgagaaa atcaaagcct tcctcgatat gcacaatttt
     8761 ggttttttcc tgtgacaatg aaagacctcg ttgtgcaaga aagtttgaaa ttatcggcat
     8821 cactttttct ttgaggatat caggactgtt tgcagttacg atgaaatcat cggcaaaacg
     8881 aatataattt attttattgc cttgcccaca gccggagtga atttccctct caagtccgtc
     8941 tagaaccata tttgcgagca gtggtgaggc tggaccacct tgcggagtac ctcgtgctgt
     9001 ttcaaataat tgattctttt ccatgtagcc tgcctgcaac cactgtgtta gcactcgttg
     9061 atctattatg atattacttt tgagccatcc atgatcaatt tcatcaaaac aggccttaat
     9121 gtcgccttct aaaatccatt gagcgcaatt tttccgtgcc aaggttgcat agcactggta
     9181 tatcgcgtca tgagtacttc gctttggtct aaatccatag gaatttagat cagctgtagt
     9241 ctctccaacg ggttctaatg ctaacaaata gagtgcttgc attgcccgat ctttcaaagt
     9301 gggtatgctt agtggacgct cttttccatt cttcttcggg atgtaaatcc ttcgaagcgg
     9361 agttgcctta taccctcttg cttttaggtt gcgcaccgcc tcgcattttt cttctgatgt
     9421 tgtccaaaca acaccatcaa taccaggcgt cttgcttcct tttgctgtcg ttactcgtct
     9481 cactgcaaga agttttgcgc tgatggaatt aaccagcagc cattgcaatg acttcacttt
     9541 tccgtgctgt ttattactca ctgcctttgc gatacgcact tgaagtctgt atacattttt
     9601 ctcaatacta ggccagtcga gctgatccca ctttgtattt aagatgaatg cgtcaggcgc
     9661 accagataat aaattatccg tcatttgctt tcctcacttc tacaaattct gcaaattctt
     9721 tcgcaatgaa agaccaatcg gacgtgggct tcttttcaga ccgggtgaca ttgaaacccg
     9781 tatccaactc attacaaatt ggccttcgct ttttccgata tcccaagcct acacttccat
     9841 aggcagcctt tgcaggctac tgtcttctta cgaagagaaa tatagggttt ccaagttccg
     9901 ctgttcaagt aatgccgggt taggtgtctg ctctcggccg gaaagtttcg taatcgtgtg
     9961 gggttcgtgt gatttcccca gctacctttc gtaccttttg gttcaagcga ttcagccaat
    10021 tgcgcttgtt gcgagttacg acctttatca cagattcact tatgttcacc ataccagcta
    10081 cctagtagct atcccgattt tggctatcag gtagatttac ttcctttcgg atgttaacat
    10141 ctcactatca gcccttcagc tgtagtttca ctacattgtc agggaggctt tattaactcc
    10201 cgtaggttca tctgacgata caaatggttt tcgaaattcc ttccgattaa caactcaaaa
    10261 cagcgactta tcttgtcgca ccacaccttt cgcaactggt tgctgtgtgt gtaactcatt
    10321 gtatttattc aatataaaat catatttgca agaatcggct acgaaacact aaatatttgc
    10381 taaatagcaa tcaaaaacca gtatgcaaat gtgcatcact tttaaatatt ttgtcagatt
    10441 tatctgatgc agatctgcat actgacactc gtaaccagtt gaaatatata gcctaatatg
    10501 atctaattgt aattaggggt ggcgtgcatg gatgcatcag ccagacatcc aacacaatcg
    10561 aaaaagaggt tatattatgg ctagaatcac cgtgacctta cctgacaatc ttcataaaca
    10621 aataattaaa atagccggta aggagaatga ttcactctct tatacgacaa cacggcttgt
    10681 tgaaattgga ctgatggtga tgaacagtaa gtctgataat aaagatgagc agaagactac
    10741 aaacattgaa gagtattgcc aaaaactcat tattcaaatt aatggcatca tcaaagaaat
    10801 tgccgttgat aaatttaatt ttgatgatga taaaatcgtt caaataacca aagatacctt
    10861 aaacaaattt aataaattaa aaggtattca acaggaatca ctatagcttt atcttgccct
    10921 tagactttta acatactcct cataatccgt tggtcctagg ttcaagtcct agtgggccca
    10981 ccaataaaat caagtagtta cgagtagtaa aaactgaaac ggtctgaggg gcactgatgg
    11041 ggcactcaga aataacaaat agcaactcat cccaataaca tggggcattt aaaataaatt
    11101 cctttacctg ctatttttat caatttcctc acttaacaag tgtatagcct cattaatttc
    11161 ataagtaatt gccaaagaat cctctttaat atcaaaatca attttaccac caccatggcc
    11221 agcatattta cttcttttag cttgcaagca ccttagtgct ttcattatct tatctgcatt
    11281 tggagaaccc cagtttttca aaagtatctc caaacactca aggctcctct tattctcttc
    11341 agcatttact aatctacgaa tgaccttttc ctgtaatcca tcaataacta aaatagtcaa
    11401 attcaacaaa taatctaaat actctttttt gatatcagta ataactgggt gaatctgctt
    11461 taatagcata tcaatatcac cccctttagg agcccataat acaaattttt ccttatctaa
    11521 agaataagga ggaaaattgg ctaatttttg ttttatcgct ctaagagaac tttcttcttc
    11581 atcgaattca gctaaaaaat ccgtgttata agcgactttt gaaatccccg cttttggctc
    11641 ttcattatac tgcttccaat gaagctgttc tttatggggt aattgcccta aacaataaat
    11701 ataagcgtgc acctgtcctt catcatttat tgagtaagac ttcaaactcc aacttccata
    11761 gcaatgaatt ccccgatctc tgacctcata tttctcatga ttatttttat atttagacaa
    11821 tacttctgga ttaaaaaatg ctgggcttgt ctgaaaaggt ttaccagtat ctttatagta
    11881 attatcaagc atttctggag cacaagacca ttcgactatt ttactatttt taaaatcagt
    11941 aataataaaa ttctcgtatt tcttaggatc gcctttttca atctgctcaa ttgaatagac
    12001 tgtttggatt acctctgcac ctttaaaccg tggtatatct tcatattcat aaattcttcg
    12061 tttgattctt gaagtttgta aactacta
//
LOCUS       DAXLDT010000095        12081 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXLDT010000095 DAXLDT010000000
VERSION     DAXLDT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.095"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..695)
                     /locus_tag="ACF2SP_002066"
     CDS             complement(<1..695)
                     /locus_tag="ACF2SP_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFD2359842.1"
                     /translation="MTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSM
                     LKSMRMGQHFLDESSPFSGENEATFQEMLDAQYASTIAESKGIGLAALLAKQLENSVG
                     YKANSLDNSSAEVNNTKVTNSEESISVVDDFVKSVWPTAKKAASLIGLDPKLLMAQAA
                     LETGWGKFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYP
                     SIEHSFHDYVSLI"
     gene            complement(707..1810)
                     /locus_tag="ACF2SP_002067"
     CDS             complement(707..1810)
                     /locus_tag="ACF2SP_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFD2359843.1"
                     /translation="MRRILVIRWILAIHLIATQVFAERIKDIATLAGVRVNQLVGYGL
                     VVGLSGTGDKTGTKFTEDSFANMLTQLGINIPPGVRLNSKNIAAVMVTANLSSFMKKG
                     QTMDVNISSIGDSKSLLGGTLLLTPLKGADGRVYAMSQGNIVVSGISASGSDGSSVTV
                     NVPSGGRIPNGATIEADIPNPFYYSNSLTYNLHTPDFTTAKRMSDAINELMGPGTAKA
                     IDAGSVVVTAPKKLSQRVDYVSVLENIEFKPGEPMAKIIINARTGTVVISSNVIVKSA
                     AVSHGNLVVSITETPVISQPNAFASGRTVATQQSQVNIQQKNNRAFILPKGTTLKDIV
                     RGINAVGATPADVISILEALQQAGALSATLIVI"
     gene            complement(1849..2541)
                     /locus_tag="ACF2SP_002068"
     CDS             complement(1849..2541)
                     /locus_tag="ACF2SP_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFD2359844.1"
                     /translation="MNRLNIAVSCLATALLFGCEALHPPAPGDNPDYAPTYPVTPDPK
                     ELRKVSGAIYSSETALPLFETPRARHPGDILTVFLIEKTDAQKNATTTQRKNDTTKIT
                     NKLFLGRPISLGSGYSMDFDLDNQRQFNGEGRSIQNNKLAGSISVTVAKVLANGNMVV
                     QGEKWVRINQGNEFVRLSGIVRPQDIKADNTITSDRIANARISYGGTGQINNTNAQGW
                     LSRILWGPLFPT"
     gene            complement(2554..3339)
                     /gene="flgG"
                     /locus_tag="ACF2SP_002069"
     CDS             complement(2554..3339)
                     /gene="flgG"
                     /locus_tag="ACF2SP_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFD2359845.1"
                     /translation="MEPALWVSKSGLEAQDKNIATIANNLANVNTTAFKKGRALFEDL
                     IYQNLRQAGAQSSQNSQIPTGINMGTGVHLVATEKIFSQGSLQNTQNALDVAIEGRGF
                     FTVLMPDGTQAYTRDGSFKTDSTGQIVNSNGYPLQPNITIPPQTTKITIGTDGTVSAL
                     VAGNNAPSVIGNIQLTDFINPAGLQPIGQNLYTETVASGTPITDNPGNSGLGTLLQGS
                     LEASNVNVVEELVNMIQAQRSYEITAKSIQTVDSMLQYLTQTL"
     gene            complement(3490..4236)
                     /gene="flgF"
                     /locus_tag="ACF2SP_002070"
     CDS             complement(3490..4236)
                     /gene="flgF"
                     /locus_tag="ACF2SP_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFD2359846.1"
                     /translation="MEPILYKAVSGGRSDFKRQEMIANNLANINTPGFKADLYQAQTM
                     YMNNANGNNQFNMHSFITQNTNGVDTSPGEIITTGRDLDVAIDGDGWMAVQDSQGREA
                     YTRGGSLRLNANGQLITASGKVVLGDGGPISIPPAQSIEIGSDGTISIVPLEGDVKSL
                     AILDRIKLVTLDRNNIYKNDEGLIQLRSGAATTNSNIKLQSGALEGSNVNAIDQMVAM
                     ISAGREFDAQMKLLSTVDDNGQKLAQLLQE"
     gene            complement(4247..5560)
                     /gene="flgE"
                     /locus_tag="ACF2SP_002071"
     CDS             complement(4247..5560)
                     /gene="flgE"
                     /locus_tag="ACF2SP_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFD2359847.1"
                     /translation="MVFNTALSGIQASTRDLEVIGNNIANSATIGFKGSRAEFADIYS
                     SSAYGSGGNAVGGGVKLSRVHQSFATGTLSTSNNTLDLAVNGSGFFILSDQGAKVYTR
                     AGQFKLNNENYIVNANNQRLTGLLTDSNGAITGASGDLRINTANIPPRASSNVSVGVN
                     LSSQSKAPNVDWVGGASPATDTYNNVTSSTIYDSLGNSHVLSMYFIHADSTALAGSPN
                     AASPAGTENQWYVAFQIDNQNVPPNVGAQNTNNLFRANFNSDGSFAGVFDTSNAALPN
                     NLIPLSFNLNNGSNPLSLSIDLSDSTQFGSPFAVQSTFNNGYTTGSLAGLDIDDSGVI
                     YGRYTNGQSLSMGQIQLANFADPESLQNLGNTNWAETTSSGQPLISAPGSGGLGLINS
                     GRLEESNVDLTSELVNLISAQRNFQANAQTIRTGDAITQTIINIR"
     gene            complement(5674..6351)
                     /gene="flgD"
                     /locus_tag="ACF2SP_002072"
     CDS             complement(5674..6351)
                     /gene="flgD"
                     /locus_tag="ACF2SP_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFD2359848.1"
                     /translation="MTTNSVNGSNSIGGLGLNQIDSDKRQNLGQQDFLRLMVAQVQNQ
                     DPLQPQANGEFLSQLAQFSTNDGITKMQESLQQLASSLQSNQALQASALVGRKVLVNS
                     NTLSLGTEGDVKAAIDMAPGVSNLRAAIYTESGELIKTIPLGQPEPGFFQFSWDGTDQ
                     SNQRLASGRYTIKVTGTYGGQEVALKTMTSANVDSVSLGQNGEDLKLNVAGVGSVYLS
                     QVRQISV"
     gene            complement(6362..6784)
                     /gene="flgC"
                     /locus_tag="ACF2SP_002073"
     CDS             complement(6362..6784)
                     /gene="flgC"
                     /locus_tag="ACF2SP_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFD2359849.1"
                     /translation="MSLNAIFDIAASAMTAETVRLTTSAGNMSNANVEAGSPDEVYQA
                     KYPVFKSVQENANQWIGEQIKAGVKLDGIYQSDAEPHRRYDPNSPIADKDGFVYTSNI
                     NYVEEMANIISASRSYQMNIELLNTTKQLMQRTLQLGE"
     gene            complement(6787..7179)
                     /gene="flgB"
                     /locus_tag="ACF2SP_002074"
     CDS             complement(6787..7179)
                     /gene="flgB"
                     /locus_tag="ACF2SP_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFD2359850.1"
                     /translation="MAINLDSYFGIHAKALIARDQRASVLANNIANVNTPNFKARDVD
                     FNEVLTATMAGNSQQLRVTSDQHISTNADFMTHLKYRMTHHASLDGNTVDKDMETTEF
                     ARNALNYQASLSFLNGKIKSMLTALKGE"
     gene            7395..8330
                     /gene="hemF"
                     /locus_tag="ACF2SP_002075"
     CDS             7395..8330
                     /gene="hemF"
                     /locus_tag="ACF2SP_002075"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFD2359851.1"
                     /translation="MSISKTLPYNAIEQIKSYLLQLQNTICVLLESIDGKTRFHEDSW
                     QRAAGGGGRTRIMTNGNVFEKAGVNFSHVSGEQLPASASAHREELAGRHFSALGVSLV
                     IHPQNPYVPTTHANVRFFVAEKEDSEPVWWFGGGFDLTPYYGFVEDCEHWHQTALNAC
                     LPFGETIYPKFKRWCDDYFFIKHRNEARGIGGLFFDDYNEISFDHSFELMRSIGDHFI
                     LAYEPIVARRKDIPFGNREKAFQNYRRGRYAEFNLVYDRGTLFGLQSGGRTESILMSL
                     PPTVHWEYNWHPEKGSDEEKLYTDFLPAKDWLKKE"
     gene            complement(8475..9782)
                     /locus_tag="ACF2SP_002076"
     CDS             complement(8475..9782)
                     /locus_tag="ACF2SP_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2359852.1"
                     /translation="MTTDFIGLLKQRKFLPLFLTQFFGAFNDNAYKLAMLTMISYHLS
                     HTQEQSEYYQAIAGALFILPFFIFSATSGQLADKYDKALLTRLVKVLEVLLMIIGGIA
                     LYSGSIFLMMCTLTGMGVHSSFFGPIKYAILPDHLPKKNLLAATGLIEASTFIAILLG
                     TTLGTLTVGGKEAIPYIAIIMTLSAAFSGLVSSLFIPPAPSALHELKVDFNLWRATVK
                     MLKEAKKEYGILISILTISWFWLIGAVMLTKLPDYTHYVLGASAHVFALFLALFSIGI
                     ALGSITINWLLKGQVNLSAVPMAMLAFTFFAFDLYWSSPAGHDLKTPLFNLFNFFTSF
                     THWRIAFDLFMLAFCAGLFVVPLYTYLQIISPVENRARTIAANNIFNALFMVMGTLLV
                     MLLLFFSFSIPQVFLILCLLNLVAAVFLFLGLRTLRRSSEEIA"
     gene            10132..10557
                     /locus_tag="ACF2SP_002077"
     CDS             10132..10557
                     /locus_tag="ACF2SP_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HFD2359853.1"
                     /translation="MAKNSANDYSALLDIIRSHTPVRYNELNIPLTITQKHAEAVLHV
                     VHDVGGQPDGPLDKSTHKPTLWEKNTHCTAECLAWRGHWVAEERRRRENDLGETIYFG
                     TPYYARWLLAMAKMLLDKGYITPDELMNKMAEVRKREEI"
     gene            10558..10866
                     /locus_tag="ACF2SP_002078"
     CDS             10558..10866
                     /locus_tag="ACF2SP_002078"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HFD2359854.1"
                     /translation="MDKYKIGDRVTVSNLPSLFHTRTQGYTRGHTGEIVLLRPEWVIP
                     EDEAWGRYEGRRERFYLVRFKLNELWEDYGGSPIDTLETEFSERWLEPAKENSKKEKK
                     "
     gene            10866..11534
                     /gene="scnC"
                     /locus_tag="ACF2SP_002079"
     CDS             10866..11534
                     /gene="scnC"
                     /locus_tag="ACF2SP_002079"
                     /EC_number="3.5.5.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiocyanate hydrolase subunit gamma"
                     /protein_id="HFD2359855.1"
                     /translation="MKEEHLHEPIEEHNEAIEFNILETAIRELAIEKGLFSREDHRRF
                     TEWYDNLGYYSGSRVVARAWVDPEFKKRLLADGVAACKELGIDWLKPTGEGTPSDYTH
                     LIVCENTPKLHNTMVCTLCSCYPRPLLGIAPAWYERPNYRRRLVRWPRQVLAEFGTII
                     PPDVEVRVHDSNQRTRFMILPMRPEGTEDWSEEKLATIVTRDTMIGVAIPRIDWTVDS
                     IPGT"
     gene            11548..>12081
                     /locus_tag="ACF2SP_002080"
     CDS             11548..>12081
                     /locus_tag="ACF2SP_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HFD2359856.1"
                     /translation="MFKMSLRTVFVAVIAILMPSVVFAHHAEFMSKNPFWQGFSMPIH
                     GIDHMLFSLAVGLIAAQIGGRALWSVPLVFSVAMLLGSILNVSGVAIPLLQYGILASL
                     VICAALLSWRTSVSLLLTVGLIGLFAIFQGNAMTTEYNLVYSMPFFIAGCLISSLILL
                     SIGVGLGLLISYLNKSIF"
BASE COUNT         3637 a         2532 c         2284 g         3628 t
ORIGIN      
        1 ctaattaatg aaacatagtc atgaaaacta tgttctatgg atggatattt tctaaaggaa
       61 gcgttgatct taatgggtgt atcggcaatg tattctgttg ttttaacctg aattgattct
      121 acttcgctat gcgacccggt ttttatatta aataaattat tactgctcga accatctgca
      181 tctcttgtaa caaatttacc ccagccagtc tctaatgccg cttgcgccat caacagcttg
      241 ggatccaatc caattaatga tgcagccttt tttgctgtgg gccacacaga ttttacgaaa
      301 tcgtcaacaa cagatatcga ttcttctgaa ttagtcactt tagtattgtt aacctctgca
      361 gaagagttat ccaggctgtt tgctttatac ccaactgaat tttccagctg tttagccagt
      421 agagccgcca atcctatccc tttagactcg gcaatagtac tagcatattg tgcatccagc
      481 atctcttgaa aagttgcttc attctcgcca ctaaaaggac tcgattcatc aaggaaatgc
      541 tgccccatcc tcatgctttt taacatggat tgcaagaata ttccttcaaa ctgtttagct
      601 acctcaggca acgcttcttt tgcattgttt tttgcctgaa tttttaattc atttaaccct
      661 tgaaaatcac tcgtagcaat actttgtatg gtcatatcca tcacctttat atgactatta
      721 atgtagcact caatgcgcct gcctgttgta aagcttccag tatggaaatc acatcagcag
      781 gtgttgcacc aacagcattg attcctctga ctatatcttt caaagtggta cccttgggta
      841 agataaaggc acgattattt ttttgctgaa tattgacttg tgattgctgc gtagccaccg
      901 tgcgaccgga agcaaaggca ttaggttgac tgataacggg agtctctgta atgctaacga
      961 ccaaattacc atgagatacg gcagcagatt taacaatcac attgcttgaa ataaccactg
     1021 ttccagtcct ggcattgata ataatttttg ccatgggttc tcctggttta aattcaatat
     1081 tctcaagtac tgaaacataa tctacgcgtt gagataattt tttaggggct gtgactacca
     1141 cagacccagc atctatagct tttgctgtgc caggccccat taactcattg atagcatcac
     1201 tcattcgttt cgctgtagtg aaatcggggg tatgcaggtt gtaagtcagt gagtttgagt
     1261 aataaaatgg attgggaata tcagcttcaa tcgtagcgcc attgggaatt cttcccccgc
     1321 tgggtacgtt aacagtaaca ctggaaccat cgctcccgga agcgctgatt ccagagacta
     1381 caatgtttcc ctgtgacatg gcataaacac gaccatccgc tcctttcaaa ggagtaagta
     1441 ataaagttcc acctaataaa cttttagaat cgcctatgga cgaaatgttg acatccatag
     1501 tctgtccttt tttcataaaa ctagacaaat tggcagtaac catcactgct gcaatgtttt
     1561 tggaattgag tctaactcct ggaggaatgt taattcccaa ttgagtcagc atgttagcaa
     1621 aactgtcttc ggtaaatttt gtgcctgtct tatccccagt gccgcttaaa ccaaccacca
     1681 acccataccc aaccaattgg ttaacacgaa caccagccaa agtagcgatg tctttgattc
     1741 gttcagcaaa aacttgggtt gcaatcaagt gaattgccaa tatccatcga ataaccagta
     1801 ttcgccgcat cttaattcct tgtcgttaat aatctcgata attaccaatt aagtcgggaa
     1861 taaaggcccc cataaaatcc ttgataacca tccttgagca ttcgtattat taatttgacc
     1921 agttccacca taagaaattc tcgcattggc aattctatct gaggtaatag tgttatctgc
     1981 ttttatatcc tggggacgaa ctatccctga aagacgaacg aattcattcc cctggttaat
     2041 tctgacccac ttctcgcctt gaacaaccat attgccattc gccaacactt tggctaccgt
     2101 cactgaaata ctgccagcca atttattgtt ttgtattgat ctgccttctc cgttaaactg
     2161 cctttggtta tccaaatcaa aatccatgct gtaaccactt cccaaagaga tgggtctacc
     2221 caaaaagagt ttatttgtga tttttgtcgt atcatttttt ctctgagtgg tagtcgcatt
     2281 tttctgagca tcagtttttt cgattaaaaa cactgttaat atatcacccg gatgccttgc
     2341 cctaggtgtt tcaaacaaag ggagagccgt ttcactacta taaatagcac cacttacctt
     2401 tcttaattcc ttgggatccg gagttacagg ataagttgga gcataatcag ggttgtcgcc
     2461 tggtgccggt gggtgtaagg cttcacaccc aaacaacaaa gctgtagcta aacaagagac
     2521 agctatattt aatcgattca taacctatct cacttacaga gtttgggtta aatattgcaa
     2581 catgctgtca acggtctgaa tgctttttgc tgttatttca taacttcttt gagcctgaat
     2641 catattaacc aattcttcta ccacattcac attagaagct tctaatgaac cttgcaataa
     2701 agtacctaat ccggagttgc cagggttatc tgtaatcggt gtaccgcttg ctacagtttc
     2761 cgtatataaa ttctgcccaa tgggttgcaa ccccgctggg tttataaaat cggttaattg
     2821 gatatttcct ataacggatg gcgcattatt tcctgcgact aaagcactaa ccgtgccatc
     2881 tgttccaata gtaatttttg ttgtttgtgg cggtatcgta atgttgggct gaagaggata
     2941 cccattacta ttaacaatct ggcctgtact atcagtttta aagcttccat cacgtgtata
     3001 ggcctgtgtg ccatctggca ttaagactgt aaaaaagcca cggccttcga tagcgacatc
     3061 caacgcattt tgtgtattct gaagactgcc ctggctgaat attttttctg tagcaaccag
     3121 atgcactcct gtccccatgt ttataccggt aggaatttgc gaattttgtg aggattgagc
     3181 accagcctgg cgaagatttt gataaattaa atcctcgaat aaagctctac cttttttaaa
     3241 agcagttgtg ttgacgttag caagattatt cgcaatagta gcaatattct tatcctgggc
     3301 ttccaaacct gatttactca cccataatgc tggttccata acggcctcct gctattattc
     3361 ttattatatt atattggcct taattatcat ttcagtctat caacaccttg cgctcctgaa
     3421 accatctata gttcctaatc attggaaagc ggcttcccct aactgaaata ataattaaat
     3481 taattaagtt tattcttgca atagctgtgc caatttttgt ccattatcat cgactgttga
     3541 taataacttc atttgagcat caaattcacg accagcacta atcatagcta ccatttgatc
     3601 aatcgcattt acattactcc cctccaatgc accgctttgc aatttaatat tactattagt
     3661 ggtagccgca cctgatctca gttggatcaa accttcatca tttttataga tattatttct
     3721 atccagcgta actaacttaa tcctgtctaa gattgccaga gatttgacat cgccttctaa
     3781 agggacaatc gatatagttc cgtccgaacc aatctctatg gattgagcag gcgggataga
     3841 aatgggacct ccatcgccca aaacaacctt acccgaggca gtaattaact gtccattagc
     3901 attcaaacgc aaactacccc ctctggtata agcttcacga ccttggctgt cctgaacagc
     3961 catccacccg tctccatcaa ttgctacatc caaatctctg cctgtcgtaa taatttcccc
     4021 aggagaagtg tccactccat tggtattttg agttataaat gaatgcatat tgaactgatt
     4081 atttccattt gcattattca tgtacatggt ctgtgcctgg tacaaatcgg ccttaaatcc
     4141 aggggtgtta atatttgcca aattattggc gatcatttcc tgccttttaa aatctgaacg
     4201 accgccagaa acagccttat acaggatagg ctccatagct tacctcctag cgaatattga
     4261 tgatcgtttg agtaatcgca tcgcctgtac gaatggtttg agcattcgct tgaaaattgc
     4321 gctgggcact aattaagtta accaattcac ttgtcaaatc cacatttgat tcttcaagtc
     4381 ttcctgaatt aattaatccc aaaccaccac ttccaggagc gctaatcaat ggctgtcctg
     4441 atgaagttgt ttcagcccag ttagtatttc ctaaattttg caaactttca ggatctgcaa
     4501 aattagcgag ctgaatctgg cccatggaca aagattgtcc attagtataa cgaccataga
     4561 tcactcccga gtcatcaata tccagaccag caagacttcc ggtagtgtag ccattattaa
     4621 aagtcgattg aacagcaaaa ggactgccaa attgagtact atctgataaa tcaatactga
     4681 gacttaaagg attggaacca ttgtttaaat tgaaagatag gggaattaaa ttattgggta
     4741 atgcagcgtt tgatgtatca aatactcccg caaacgagcc atcagaatta aagttagctc
     4801 ggaataaatt atttgtattt tgagctccaa cattaggagg aacgttttga ttgtctatct
     4861 gaaatgctac ataccattga ttttctgttc cagcaggtga tgcggcattg ggtgaaccgg
     4921 ctagggctgt tgaatcggca tgaataaaat acatgctcaa tacatgtgag tttcctaaac
     4981 tgtcatagat tgttgacgag gtgacattgt tataagtatc ggttgccgga ctagcccctc
     5041 cgacccaatc tacattcggc gctttgcttt gagagctcag attcacacct acagagacat
     5101 ttgagctggc tctgggagga atatttgcgg tgtttattct taaatcacct gatgcgcctg
     5161 taatagcacc attagaatca gttagtaaac cagtcaatcg ttgattattt gcattgacta
     5221 tatagttctc attattcaac ttgaattgcc cagcccgagt ataaaccttg gctccctgat
     5281 cacttaatat aaagaaaccc gaaccattca cagccagatc gagcgtattg tttgaggtac
     5341 ttaatgttcc tgtagcgaat gactgatgta cacgcgagag tttaacgcca ccacctacag
     5401 cgttgccccc agaaccataa gcgctggatg aataaatatc tgcaaactcg gctcttgaac
     5461 ctttaaatcc tattgtggca gagtttgcaa tattattacc tataacttcc aaatctctgg
     5521 ttgatgcctg aattccactt aatgctgtat taaaaaccat aatagcctcc tgctttataa
     5581 ttttttagca tttgcccttg actgctttat aagcaatgcg cttatgactt aagccatcgt
     5641 ttcaataagg ccacggtgac taaaatcatt gatttatact gatatttgcc ttacctgact
     5701 taaatagact gatcctacgc ccgctacatt aagctttaaa tcctcgccat tttgcccaag
     5761 actgacacta tcgacattgg ctgacgtcat agttttcaat gccacttctt gtccgccata
     5821 agtaccagta acctttattg tatatcttcc tgatgctaaa cgttgattac tttggtcagt
     5881 gccatcccaa ctaaattgga aaaacccggg ttctggttgc cccaagggaa tagtttttat
     5941 caactcgcca gattccgtgt agattgcagc acgaagatta gaaacaccag gtgccatatc
     6001 aatggcagcc tttacatctc cttctgtacc cagactcagg gtattgctgt ttaccaacac
     6061 cttgcggcca accaaagcgg atgcttgaag cgcttgatta gattgtagag atgaagctaa
     6121 ttgttgcagt gattcttgca tttttgtgat tccatcattg gtgctaaact gagccagttg
     6181 agataaaaac tcgccatttg cctgtggttg cagaggatct tgattttgaa cttgtgccac
     6241 catcaaacgc aaaaaatcct gttgtcccaa gttttgtctt ttatctgaat caatttgatt
     6301 caaacctaaa ccacctattg aattggaacc atttactgaa ttggtagtca ttcccttccc
     6361 cttattcgcc taattgcagc gtcctttgca taagttgttt ggttgtatta agtaattcaa
     6421 tattcatttg ataagaacga gaagctgaaa tgatatttgc catttcttca acgtaattga
     6481 tatttgacgt atagacaaac ccgtctttat cagctattgg actatttgga tcgtaccttc
     6541 tgtgcggttc tgcatcactc tgataaatac catccagttt cacgccagcc ttaatttgct
     6601 ccccaatcca ttgatttgca ttctcctgga ctgacttgaa aacaggatat ttggcttgat
     6661 agacctcatc tggactacct gcttcaacat tcgcattact catattgcca gcactggtag
     6721 tcaaacgcac tgtttctgcg gtcattgcag aagccgcgat gtcaaaaatt gcatttaatg
     6781 acatgattac tcccctttca atgcggtcag catggacttg attttgccat ttaagaaact
     6841 caagctggcc tgataattca atgcatttcg agcaaactcg gtagtttcca tatctttatc
     6901 aacggtatta ccatctaaag aagcatgatg tgtcattcgg tatttcaaat gagtcataaa
     6961 atcggcattg gtactaatat gctggtccga agttaccctt aactgctggc tgttaccggc
     7021 catagttgct gtcagtactt cattaaaatc aacatcccgg gctttaaaat tcggtgtatt
     7081 aacattggct atgttattag caagaactga tgccctctga tctcttgcaa tcaacgcctt
     7141 ggcatgaatc ccaaaataag aatcaagatt aatggccata tttactcctg tcttattgtt
     7201 atttaagtct taatcatcat ttgacattat gccaattttc caaagcacta tatggattca
     7261 tacttgtttc caaacttcac tgaaacataa aaatgataaa aaaatgctat ttaaacaaag
     7321 caaacattgt gccaatatcc aaaaaatgag aaaatgaaac atccaagcgc taattcccta
     7381 tgtggagtcc catcatgtca atctcaaaga cacttcctta taatgcgata gagcaaatta
     7441 aatcttatct gctccaactc caaaatacaa tttgtgtctt actggagtca attgatggca
     7501 aaaccagatt ccatgaggat tcctggcagc gagctgctgg aggtggagga agaacaagaa
     7561 taatgacgaa tgggaatgtc tttgaaaaag ccggcgtcaa tttctcacat gtttcagggg
     7621 aacaattacc agcatcagcc agtgcacata gagaagaatt ggcaggaagg catttcagcg
     7681 cattaggggt atctctggtc attcatcctc aaaaccccta tgtgccaaca acacatgcca
     7741 atgttcgatt ttttgtcgct gaaaaggaag attcggaacc agtctggtgg tttggtggag
     7801 gatttgattt aactccttat tatggatttg ttgaagactg tgagcattgg catcaaacgg
     7861 cattaaatgc ctgtttacct tttggagaaa cgatttaccc aaaattcaag cgttggtgcg
     7921 atgattattt ttttattaag cacagaaatg aagcacgtgg aattggcggt ttattttttg
     7981 atgattacaa tgaaatcagc tttgatcaca gctttgagct catgcgtagc ataggcgatc
     8041 attttattct ggcttatgaa cccatagtgg ctcgccgtaa agacatccct tttggcaaca
     8101 gggaaaaagc atttcagaat taccgcagag gccgttatgc ggagttcaat ttagtctatg
     8161 atcgaggcac tttatttggt ctgcaatctg gcggaaggac tgagtctata ttgatgtccc
     8221 tgcctcctac tgttcattgg gaatacaatt ggcatcctga aaaaggcagt gatgaagaaa
     8281 aattatacac agatttttta cctgcaaaag attggttgaa aaaggagtaa aacaccaaat
     8341 tcaaaaataa atgatttcct tctcccctta cctgaaaatt tcgaagagcc agtatacatg
     8401 cagtaaatga gcaccgcgaa acgtttacaa cgcgcaataa aatgaaagag acaggttatg
     8461 caagagatct gttgttaagc tatctcctct gagcttcttc ttaaggttct taacccaaga
     8521 aacaagaaca ccgcggcaac caaatttaaa agacaaagta tcaaaaaaac ctgaggaatt
     8581 gaaaagctaa aaaacaataa cagcatgact aacaaagttc ccatcaccat aaacaaggca
     8641 ttgaaaatat tgttcgccgc aatcgttcta gccctgttct caactggact tataatttgc
     8701 aagtaagtat acaagggaac tacaaatagt cctgcacaaa atgctaacat aaataaatca
     8761 aaagcaattc gccaatgagt aaatgaagta aaaaaattaa ataagttaaa taagggggtc
     8821 tttaaatcat gacccgcagg agatgaccaa tacaaatcaa aagcaaaaaa agtaaaagcg
     8881 agcatggcca ttgggacagc agaaagatta acttgtcctt tcaataacca atttatagta
     8941 attgatccca gagcaatgcc tattgaaaat aaagcaagaa atagagcaaa aacatgagcg
     9001 ctagccccca aaacataatg ggtataatca ggtaatttgg taagcatgac agcgccaatt
     9061 aaccaaaacc aggaaatagt taatatggat ataagaattc catattcttt tttcgcctcc
     9121 tttaacatct tcactgtggc ccgccataaa ttaaaatcca cttttaattc atgaagtgct
     9181 gatggtgcag gaggtataaa caggctggag accaaaccag aaaaagcggc acttaaggtc
     9241 ataataatcg cgatataagg gatagcctcc ttccctccaa cggttaacgt acccaatgta
     9301 gtgcctaata aaatagcaat aaaagtgctg gcttcaatta ggcctgtcgc tgctaataaa
     9361 tttttcttag gcaaatggtc tggtaaaatc gcatatttga taggtccaaa gaaagaagaa
     9421 tgtaccccca tccctgtcaa agtacacatc atcaagaaaa tgctaccact gtataatgca
     9481 atacctccaa taatcattaa taacacttca agcaccttaa ccagacgagt caacaaagcc
     9541 ttgtcatatt tatctgccaa ttgtccggaa gtagcggaaa agataaagaa aggcagaatg
     9601 aataaagcgc ctgcaatagc ctgataatat tccgactgtt cttgtgtatg gcttaaatga
     9661 tagctaatca tagtcaacat cgctagctta taagcattat cgttaaaagc accaaaaaat
     9721 tgggtcagga aaagaggcaa aaattttctt tgtttgagaa gaccgataaa atctgttgtc
     9781 attaaagttc catttgcgct ttaaaactaa agttaacaag gatattgctg tattgatcga
     9841 aagtaaatga caaaagcctg agaaaaattc agaaaatatt atccacacaa agaccaattg
     9901 tatatattca ataaaatata aatagaaact gacttggaac gcaaatactc agtacctatt
     9961 acccagtccc tcgtacatca atactaccga cctattgcct caatgaaaga tgttgccgaa
    10021 gaatcttgct atctaatcta attaggaaaa ctcacttaac tggtctatat ttacatatag
    10081 gagtttattt ttatttatgt tgctctaatt aatgcattcc aaggaacaag catggccaag
    10141 aactcagcta atgattactc tgctctatta gatataatca gatcacatac accagttaga
    10201 tataatgaat tgaatatacc gcttactatt acgcaaaaac atgcggaagc tgtattacac
    10261 gtagttcatg atgttggcgg ccagccagat ggccctctcg ataaatcaac gcataagcct
    10321 actctatggg aaaagaatac tcattgcaca gcagaatgtt tggcttggcg cggtcattgg
    10381 gttgcagaag agcggcgaag acgagaaaac gatctgggtg aaaccatata ttttggcaca
    10441 ccttactacg caagatggtt actcgcaatg gccaaaatgt tgcttgataa aggatatatc
    10501 acaccggatg aattaatgaa caaaatggca gaagttagaa agcgtgagga gatctagatg
    10561 gataaataca aaataggcga tcgtgtaacg gtcagtaatt tgccttcttt gtttcatact
    10621 agaacccaag gttacacacg aggacataca ggagagattg ttcttttgcg cccagaatgg
    10681 gttattccgg aagatgaagc gtgggggcga tatgaagggc gtcgtgagcg attttacctg
    10741 gttcgtttta agttaaatga attatgggaa gattacggcg gtagcccaat tgatacctta
    10801 gagacagaat tttcagagcg ttggcttgaa ccagcaaaag agaattcgaa aaaggagaaa
    10861 aagtaatgaa ggaggagcat ttacacgagc ccattgagga acataatgaa gcgatagagt
    10921 ttaacattct tgaaactgct attcgtgaac ttgcaatcga aaaaggcttg ttttcacgtg
    10981 aagatcaccg tcgctttaca gaatggtatg ataaccttgg atattattca ggttcaagag
    11041 ttgtcgctcg cgcctgggta gatcctgaat tcaagaaacg tttgctcgct gatggggtag
    11101 cagcatgtaa agaactggga atcgactggt taaaaccaac aggcgaaggt actcctagtg
    11161 attataccca tttaattgtt tgtgaaaaca cacccaaact tcataacact atggtatgca
    11221 ctttatgctc ttgctaccca agacctttat taggcatagc tccagcctgg tatgaaagac
    11281 ctaattatag acgtcgttta gtgagatggc cacgtcaagt attagctgaa tttggtacta
    11341 ttattccccc tgatgtggaa gtacgagttc acgactccaa tcagcgcaca cggtttatga
    11401 ttttacctat gcgaccagag ggtacagagg attggagtga agaaaaactt gcaacgattg
    11461 taaccagaga tacaatgata ggagtcgcca tcccaagaat tgactggaca gttgattcaa
    11521 tacctggcac ttaaagggag aaagctcatg tttaaaatgt ccttgcgtac agtttttgtt
    11581 gcagtaatag ctatcttaat gccttctgtt gtatttgccc atcatgctga atttatgagc
    11641 aaaaatccct tttggcaagg cttcagtatg ccgatacacg ggatagatca catgttattt
    11701 tccttggcag tagggctaat cgccgcgcaa attggaggtc gcgccttatg gagcgtacct
    11761 ttagtctttt ctgtggctat gctattaggc agtatattaa atgtttccgg ggtagcaatt
    11821 ccattacttc aatatggtat tttggcgtct cttgtaatct gcgcagcttt attatcatgg
    11881 cgtaccagcg tatctttgtt attgacggtt ggacttattg gtctttttgc tatattccag
    11941 ggcaacgcga tgactaccga atataattta gtatatagca tgccattttt tatagcaggc
    12001 tgtttaattt cttcattaat tctattaagc attggagtcg gcctcgggtt gcttatttca
    12061 tatttaaata aatctatatt c
//
LOCUS       DAXLDT010000096        11721 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXLDT010000096 DAXLDT010000000
VERSION     DAXLDT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.096"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..366
                     /locus_tag="ACF2SP_002081"
     CDS             <1..366
                     /locus_tag="ACF2SP_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359857.1"
                     /translation="NLNDRDKILRLFVQFTYDMHQKGILHLDNSPGNTLIRRKGNGLE
                     FCVVDINRLKIGNVSLELGISNFNRISTDHEIVEVIADEYAKLTKSNQKDCLTTLWKH
                     IKKYRAYSERKERIKKIIK"
     gene            389..1702
                     /locus_tag="ACF2SP_002082"
     CDS             389..1702
                     /locus_tag="ACF2SP_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359858.1"
                     /translation="MLYNYFRWNKYSDQPETLSDKRFKRVIYLKGAYASLKTLLISLF
                     IPLIGILRLFQRRVEMGKNINCFGLCINPDYPLPEKISPSTEQIRQMVDEINITNLLI
                     RLPLADYDHKEKYFNFIEGFSDKHILIHILQDRNHIDNPKLTKQHLREIFSRLQHITK
                     YFQIGNSVNRKKWAFISMDEYFNFFQIAQKLQKKEFPTIKLIGSSIIDFELPFIARSL
                     IHWWPIKYDGIGMQLYVDRRGAPENKQFGCDTIAKINWISSIMKISRKTVNSLWITET
                     NWPLANTKPYAPAFGNCMVSEEMQTCYLVRYFLLAIASGRVSKCYWHQLIAPGYGLVD
                     NRGLKLRKRDAYYSYKTMIKLLEGGKTISFSESTQLYRLKIENNSQMIEAVWANNVSS
                     KINVPKKTKILSIFGELIEADDTNQVEVSDYVIYLISDIESNKGS"
     gene            complement(2178..3446)
                     /locus_tag="ACF2SP_002083"
     CDS             complement(2178..3446)
                     /locus_tag="ACF2SP_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2372 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359859.1"
                     /translation="MKVKYSLFVIDKIKKYNYKRASNYRSIIMSTTIDEAIKHLDNMV
                     KLLKEFKKEVKAQKFKPEWNFENSSTIFAEFNSELNELKKLKHHNLARVIVGDLGQVI
                     EKNDLNMKLIITNKDTTQNIVELEMTSNLKKKEFYKKGIVIATDTSAINHELMKYVSE
                     VTVNCHWSRKNRTSYNSTRELVDTVANELKHEKQYPRLKRVTILGCDTADIIPPEVET
                     KSSSTMLYVYQCEKNNAATPDRKKIQSFPCFVLTISQGQYQLHYYIGQDKEETCGEMV
                     KITKQKKLQNNYKVLAVCNKNDIPLIPDSILKNIYDKTNSAKINGVNNPYLHRGATVG
                     DVQSREEWRNRKKNIPTLSCGLFKFDNKKIHNSNLAERAALIINNSRNNVKVKGYLIP
                     ITPDENHGFMKPMTIHEDTPCKAVIIDHGQ"
     gene            complement(3430..3693)
                     /locus_tag="ACF2SP_002084"
     CDS             complement(3430..3693)
                     /locus_tag="ACF2SP_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KR domain-containing protein"
                     /protein_id="HFD2359860.1"
                     /translation="MVKKYPILIIGGYGNFGKYIARVLSRDLRIALSIFGRDRQKAED
                     LVNSIKSANPITIHSCDIFQNPAETLKQVNPYKEHFSPIEGQI"
     gene            4168..5313
                     /locus_tag="ACF2SP_002085"
     CDS             4168..5313
                     /locus_tag="ACF2SP_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359861.1"
                     /translation="MITSIQDIARHVVRAGKSMVWFEFDSRKDAETALNLIKKQYSDL
                     HNLHKASVNSGNVNKHKFGFGLTHTQFKSITKFNENSQISATGLKVQASISQTNQKQS
                     PEALDKSTNRLVISTQSLDQENIALENPNTADLSMNHSNLEKVGNGHSALAIASALNS
                     PLVVLRSEQNQEKILKTVGKIGNNSLVFITGHCGKGSNSLGGLYFSDSKESMISVSRT
                     PDEIVHFLASAGLKRGDSITIVLCVCYGAKEGEYLQHHSFASRLAISLAEIGVSSTIY
                     ASKGRTTRFGKEAIVNGTITFNSGLGIKPNELVVFETMIRDSLSTIKTSQRELKAAIS
                     ISGEGLQIKHPETSPSSNNSLVGFFKPSMTIDLNPQSGNWAQRNDLK"
     gene            complement(5519..6355)
                     /locus_tag="ACF2SP_002086"
     CDS             complement(5519..6355)
                     /locus_tag="ACF2SP_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFD2359862.1"
                     /translation="MDNPYLTKLRITFAGLLFYWFLPIRKQIVLSNIDIVFKNTATHA
                     EKTRLAKAFYSHVASILKEIFRMHWLNNKKWSKRVEIHGIEHLEKAFKQNRGIILLTG
                     HLGNWELTSTIGFALLKNQFGQFNVIRRPLRAKWLERILFKKLKNSGLNIISSVDAPK
                     KISQSIKNKEIVAFAMDQHVQIENRSGVAVEFFGVKAGTYRSLAFFVHKYQTPVVPGA
                     CYRKQNGKHIMRFYPELDWEYHADKEQAIYQNTLRYNQALEQLILEHPEQWWWVHRRW
                     KL"
     gene            complement(6348..7196)
                     /locus_tag="ACF2SP_002087"
     CDS             complement(6348..7196)
                     /locus_tag="ACF2SP_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFD2359863.1"
                     /translation="MLELDRDLNCLPVTWWGRCAYFLLPYKRSKAKKNIDRVFQSSLA
                     PSEKKRLCMAYYSHIITSIKEIILYALVSKKYLERHIEIIGLEYLQSAINKGNGTLVL
                     TGHFGNWEFAPLFFLDKFNSKNHIFYCVRKNLRFAFLDSIFLRRFKRCGFKIISAKNA
                     VTQTRRALKNNGIVFFPFDIRPSSKIKNRVNADFLGQKTSTNLSLAYLARIYDSSVLS
                     ITFYRTNKRQHIIHIYPEVEGVVNSEYKQTLIENTQLYNKRLEEMLLLYPEQWLWSYK
                     RWKNNG"
     gene            7385..8512
                     /gene="lpxB"
                     /locus_tag="ACF2SP_002088"
     CDS             7385..8512
                     /gene="lpxB"
                     /locus_tag="ACF2SP_002088"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFD2359864.1"
                     /translation="MVAGELSGDLLGAGVIRELKQHLTNVEFMGVGGPQMLKEGFHSL
                     IDISELSVMGISDVLRRYPQLYLIRERLLREWTINPPDVFIGIDYPDFNLSVEARLKK
                     QHIKTIHLVSPKVWAWRQKRVHLIKKAVDLVLTLFPFEEAFYLQHGVSAQFIGHPLAD
                     LIEINPSCSALRKKYNYHSDDTILAVLPGSRVGEIKYMGPLFLEVMQRIAMERPHVHF
                     IVPIACQDLYPVFFKQLHAEYDYLKIQVIQGNAREAMAISDVVLTKSGTATLEAMLLK
                     RPMVVAFKWGILTHAIIAPQVKVPYIALPNLLAGKKLIPEFVQEKANVDSITESVLNL
                     LDSSNQNELIKQFTDIHRTLRQNANEKAALAILRILEDSLT"
     gene            complement(8484..9515)
                     /gene="lpxD"
                     /locus_tag="ACF2SP_002089"
     CDS             complement(8484..9515)
                     /gene="lpxD"
                     /locus_tag="ACF2SP_002089"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFD2359865.1"
                     /translation="MSNYQFTKPAGPFRLSELAKISGATLYEGKGETFTVSGLAKLSE
                     ATSNDLVMLHQKKYVKELKNTAARSCIIGPDYVKFAPDSMYLLVHPNPYKAFALIAQA
                     FYPSEKSSSFIAPSAKIESTALIGSDCSIAHGAYVGNHARIGKRCKIGVNTYIGDGVT
                     IGDNCIIEDNVSIRHAVIGSNVVVYPGARIGQDGFGFASDAEGHYKIPHAGGVIIGND
                     VEIGANTCIDRGSLGNTVIEDWCRLDNLVQIGHNVKIGKGSIIVAQVGIAGSTELGEH
                     VTLAGQVGVIGHLKIGKGATVLTCSKVLRNVQPGDRVIGYPAISISDWQKQIRFLKTA
                     IKSKNPLKS"
     gene            complement(9531..10361)
                     /gene="lpxA"
                     /locus_tag="ACF2SP_002090"
     CDS             complement(9531..10361)
                     /gene="lpxA"
                     /locus_tag="ACF2SP_002090"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFD2359866.1"
                     /translation="MQMSQLDHVHVANNLYTLTHQIHPTAIVSVDARIGRDVVIGPYS
                     IIEGNVSIGQGTVIGSHTSIKSWTEIGEYNQIETGAIIGAIPQDLKFSGEKSTVIVGN
                     NNIIREYVTISRGTSGGGGVTRIGNNNVIMTSAHIAHDVQMGNHNIISNAVAVAGHVI
                     IDDWVTIGGLCGIHQFVQLGRMSMIGSQTRITKDVLPYTLVCGNPAKRFGINIERLQR
                     NGYSPVARMQIQRAYKILFHDGHLLTNAIEILKREFIDNNDVAYILKFLENSKRSFYR
                     "
     gene            complement(10522..11592)
                     /locus_tag="ACF2SP_002091"
     CDS             complement(10522..11592)
                     /locus_tag="ACF2SP_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HFD2359867.1"
                     /translation="MNMKRNILLVSLPLITSLSFGGSMGEGQQDSKRFIFSASAVYGS
                     LSDSVLDKIEYADVLKASGLISSGLFNVDSRWGYSLSVGYLFGPQLDYDLVLTYTNIK
                     NHGSSIVANSNADDRLDNRLSILVQDFSGQEFVGPAVAAVNSNYDYQTADLITHRNVR
                     SKFVEQLRYTRYYGAKATQIEKGFTARYNGEAIMAFNLASLQPTSDYINFHAKYQGIG
                     PKAGGGLYWDVNRFITVGGDISLSVLAGSKKSNWNESFETIGPEPIFGFPNTGGRYAY
                     ILNHPSSVWLSPVLGANLVVASHFNLNNGGVIGVEGGIGSEQYWSMNNGENYSLANAT
                     NSVSITERLSVRNVFIKASYIV"
BASE COUNT         3720 a         2193 c         2058 g         3750 t
ORIGIN      
        1 aatttaaatg atcgagacaa aattttgaga ttgtttgtac aattcaccta tgacatgcac
       61 cagaaaggaa tactacacct cgacaacagt cctggtaata cactcattag aagaaaggga
      121 aatggcttgg aattttgtgt ggttgatatt aatagattaa aaatagggaa tgtatcatta
      181 gagctgggaa taagtaattt taatcgaatt agtaccgatc atgaaattgt agaggttata
      241 gcagatgagt acgccaaatt aactaaaagt aatcaaaaag attgtctcac taccttatgg
      301 aaacatatca aaaaatacag agcttattct gagagaaaag agagaataaa aaaaattata
      361 aaatgagtaa aatacacagg aaagtgatat gttgtacaat tattttaggt ggaacaaata
      421 ttctgatcaa cctgaaacac ttagtgataa aagatttaaa agagtaattt atttaaaagg
      481 ggcttatgcc tctttaaaaa cactattgat atcactgttc atacctttaa tcggaatttt
      541 aagactattt caacgaaggg ttgaaatggg gaaaaatata aattgttttg gactctgcat
      601 taatccggac tatccgcttc cagaaaagat atcccctagt actgaacaaa ttaggcaaat
      661 ggtcgatgaa attaacatca ctaatttgtt aattcgtctt cccttggctg attatgatca
      721 taaagaaaaa tattttaatt ttattgaagg tttttcagac aaacatattt taatacatat
      781 tctgcaggac agaaatcata ttgataatcc gaaacttaca aagcaacact taagggaaat
      841 attttctcga ttacaacaca ttacaaagta tttccaaata ggaaactctg tgaaccgcaa
      901 aaaatgggct tttatctcaa tggatgagta ttttaatttt tttcaaattg cacaaaaact
      961 tcaaaaaaag gaattcccga caataaagct tattggctct agcatcattg attttgaact
     1021 gccttttatt gctcgctctc tcattcattg gtggccaatt aaatacgatg gaataggcat
     1081 gcagctttat gtcgaccgac gaggggctcc ggaaaacaaa caattcggtt gtgataccat
     1141 tgcaaaaatt aattggatct catccataat gaagattagt cgcaaaactg taaatagtct
     1201 atggataaca gaaaccaatt ggcctcttgc caacaccaaa ccttacgctc cagcttttgg
     1261 aaattgtatg gtcagtgaag aaatgcaaac ctgctacctt gtacgctatt ttcttttagc
     1321 tatcgcaagt gggcgagtga gtaaatgcta ttggcaccaa cttatagccc caggatatgg
     1381 attagttgac aatagaggat taaagcttcg taaacgcgat gcttattata gttataaaac
     1441 catgattaag cttttagaag gaggtaaaac tatatctttt tcggagtcaa cacaattgta
     1501 ccgcttgaaa attgaaaaca attctcaaat gattgaagcg gtttgggcga ataatgtttc
     1561 atcaaaaatt aatgttccaa aaaagaccaa aatattaagc atttttggcg aattaattga
     1621 agctgatgat actaatcagg tagaagtgtc agattacgtc atttatttga tttcagatat
     1681 tgaaagcaac aagggaagct aaataaactc gcactacata atgaacaatt tatttttaat
     1741 aatggcatga ttttgaaaat ttgaactaaa tcacaatacc tagtgatggt tttggggtta
     1801 atggttgtgt attttatgca agtgaattaa ctagtttaaa atgtagtgta gcaattacaa
     1861 tctctttgga atccagcttt tcaaaaaaac gaagattaag tctaaggata ctgatgacaa
     1921 gtttattgaa ttgtagtttt attaccggaa cgagattgac tgaacaagaa cgattaattg
     1981 cattaattga gaagcatgct tcaacttcaa atcgcagagt attccacttg gattttgaag
     2041 gggattgcta cgttgttaaa atgcaacaaa agaaacgcag caagattgga tattacattt
     2101 taacaatgat tgcgaaagta ttttccatac ctgctttacg gggcattcct attctttgcg
     2161 gcaaactcac actgctttta ctgcccatgg tcaattataa ctgctttaca aggcgtgtcc
     2221 tcatggattg tcattggttt cataaatccg tggttttcat ccggggtaat tggtattaaa
     2281 tagcctttaa ctttaacgtt attccggcta ttattgataa ttaaagctgc cctttcagct
     2341 aaatttgagt tgtgaatttt tttattatca aatttaaata aaccacacga gagtgtggga
     2401 atattttttt tccgatttct ccactcttct cttgattgta catcgcctac ggtagcgcct
     2461 ctatgtaagt aggggttatt tacaccattt attttcgcgg aatttgtttt atcataaatg
     2521 ttctttaaga tactatcggg tatgaggggg atgtcatttt tattgcaaac tgctaacacc
     2581 ttatagttat tttggagttt tttttgctta gtaatcttaa ccatttctcc acaggtttct
     2641 tctttgtctt ggccaatata ataatggagc tgatattgtc cttgtgaaat tgtgagtaca
     2701 aaacaaggga aactttgtat ttttttcctg tctggggtag cagcgttatt tttttcacat
     2761 tggtatacat ataacattgt agaagatgat ttggtttcaa cctcgggagg gattatatcc
     2821 gctgtgtcgc agcctaaaat ggtaacccgc ttgagccgcg gatactgctt ctcatgcttc
     2881 agttcgttgg caacagtatc aactagctcc ctcgtgctgt tgtaggatgt tctatttttc
     2941 ctggaccagt gacaattaac cgtgacttca ctaacatatt tcattaattc atgattgata
     3001 gctgatgtat ctgtggctat cacgatcccc tttttataaa actctttttt cttgagatta
     3061 ctagtcattt caagttcaac tatattttgt gtcgtatcct tatttgtaat tatcagtttc
     3121 atattgagat catttttttc gatgacctga cctaaatctc ccacaattac tcttgcaaga
     3181 ttatgatgtt ttaatttttt tagttcattc agttcgctat tgaattctgc aaaaatggta
     3241 ctggaatttt cgaaattcca ctctggcttg aatttttgag ctttgacttc cttcttaaac
     3301 tcttttaata atttaaccat gttatctaag tgcttaattg cttcatcaat agttgtgctc
     3361 ataataattg atctatagtt tgatgctctc ttataattat actttttgat cttatctatt
     3421 acaaacaggc tatatttgac cttcaattgg gctgaaatgt tccttatagg ggttaacttg
     3481 cttaagagtt tccgctgggt tttgaaaaat atcacagctg tgaatggtta taggattagc
     3541 tgattttata ctgtttacta aatcctctgc tttttgtcga tctcgaccaa aaattgacag
     3601 ggcaattcgt aggtctcgac tgagtactct ggcaatgtat ttaccgaaat taccataacc
     3661 accaattata agaataggat attttttaac cattaaagac tacattcctt gtgagagatt
     3721 acccatatcc ctacctaggc tgttttgttt agggatgaat aatatgcccg attcattcgt
     3781 taatctctac agtgtctcag agaccgccaa gcgaaagtat tcataaaaaa caatcaatgt
     3841 gatattattg ttatcaattt aaagttgatg atttaattat gcaactaatc tttaagtcta
     3901 atattcttat tagtttccac gtagtagctc gttttccgag ccataaaatt attcccgaca
     3961 cctatctcca ataataagcg cttatttgaa ttttttaaat aaagcacgca aacaccttat
     4021 taatttattt tcaactccat cttaagaagg tttgatattt cacgttgcct atttcaggtt
     4081 aaacactgaa cagcaaatag gaaaagaacg tttttaatcg agccccggat tgagttgaaa
     4141 ccaacacatc tttacggaga taggaaaatg attacaagca tacaagatat agcacgtcat
     4201 gtagtgagag caggaaagag catggtgtgg tttgagtttg attcgagaaa ggatgctgaa
     4261 acggcactga atttaattaa aaaacaatac agtgacttac ataatttgca taaagcatcg
     4321 gtaaattctg gaaatgtaaa taagcataaa ttcgggttcg gtcttactca tacgcagttc
     4381 aagagtatta ctaagtttaa cgaaaactcc caaatatcag ctactggatt aaaagttcaa
     4441 gcttcaatat cacaaacaaa tcaaaaacaa tcaccggaag ctctggataa atccactaat
     4501 cgacttgtta ttagcactca aagcctcgat caagaaaata tagcattaga aaatccgaat
     4561 acagctgacc tgtcgatgaa ccatagtaat ttagaaaaag ttggaaatgg acactctgct
     4621 ttagcaatag cgagtgcttt aaatagtcct ctagtggtat tacgttcaga acaaaatcaa
     4681 gaaaagatcc ttaagacggt tggtaagata ggtaataata gcctggtatt tataacgggt
     4741 cattgtggta aaggttctaa ttcccttggt ggtctatact ttagtgattc aaaagaatca
     4801 atgataagcg tgagtcgaac tcccgatgaa attgttcatt ttttagctag cgctggatta
     4861 aaaagaggag atagcatcac gatagtgtta tgtgtatgtt atggtgcaaa agaaggtgag
     4921 tatctacaac atcacagttt tgcttctaga cttgcaatca gtcttgcaga gattggggtg
     4981 agttcaacta tttatgcttc taaaggaaga acaacacggt ttggaaagga agccattgtt
     5041 aatggaacca ttacctttaa ttcaggttta ggaataaaac caaatgaatt ggtggttttt
     5101 gaaaccatga taagagatag tttatcaact attaaaacca gtcaaagaga attaaaggca
     5161 gctatttcga tttcaggtga aggattacaa ataaaacatc ctgagacttc tccaagttct
     5221 aacaactcac ttgtaggatt ctttaaaccc tcaatgacaa ttgatttgaa tccgcaatct
     5281 ggaaattggg ctcaaagaaa cgatttgaag taatttacaa ctatctaata gtcaaggggc
     5341 tcttgctctt tgactccaaa ttgtttcgca aagcgcctca aaaagacatt aaataatcaa
     5401 tttgtgttaa atatgctcct ttgttatcat tacccaatga ttttaaccca atagatcttt
     5461 gttagataaa gagtagtatt aatggagatt tactatcgat aagtcaaaat ctataaggct
     5521 ataatttcca tcgcctatga acccaccacc attgctccgg gtgctctaaa attaattgtt
     5581 caagcgcttg attatagcga agggtatttt gatagatagc ttgttctttg tctgcatgat
     5641 attcccaatc cagttctgga taaaacctca taatatgttt gccattttgt tttctatagc
     5701 atgctcctgg gacaactgga gtttgatact tatgaacaaa aaaagccaag cttcggtatg
     5761 ttccagcctt cactccaaag aattctactg ccacccccga tctattctct atttgcacat
     5821 gctgatccat agcaaaagct acaatttctt tattctttat agactggctt atttttttag
     5881 gagcatcgac actgctgata atatttaaac cagaattttt aagcttcttg aacaatattc
     5941 tttccaacca tttagctctc aatggtcgtc taatgacatt aaattgtcca aattgatttt
     6001 ttaaaagagc aaaccctatg gttgatgtta attcccagtt ccccaaatgg cctgtcaaca
     6061 aaataattcc cctattctgt ttaaacgctt tttccagatg ttcaattcca tgaatttcaa
     6121 ctcttttgct ccacttttta ttatttaacc aatgcattct aaatatttct tttaaaatag
     6181 aagctacgtg ggaatagaaa gctttagcta atcttgtttt ttccgcatga gtagctgtat
     6241 ttttgaaaac aatatcaatg ttactcaata cgatttgctt gcgaattggt aaaaaccaat
     6301 agaaaagaag acctgcaaaa gtaatgcgca atttggttaa ataaggatta tccattattt
     6361 ttccaacgct tatatgacca cagccactgc tcagggtaaa gcaataacat ctcttccagt
     6421 cttttattat aaagctgagt attttctata agtgtctgtt tatattctga attaactact
     6481 ccttctactt cgggataaat gtgaattata tgttgccttt tattcgttcg ataaaaggtg
     6541 atggataata cgctagagtc ataaattcta gctaaataag ccaagctgag gttagtagac
     6601 gttttctgtc ctaaaaaatc tgcatttact ctatttttaa ttttagaaga agggcgaata
     6661 tcgaaaggaa aaaaaacaat gccattattc ttcaatgccc tacgagtttg ggttactgca
     6721 tttttagcac taattatctt gaatccgcag cgtttgaatc gacgtaaaaa aatagaatcc
     6781 aaaaaggcga accgtaagtt tttgcgcaca caataaaaaa tgtgattttt gctgttaaat
     6841 ttatcaagaa aaaataatgg tgcaaattcc caattcccga aatggccggt caatactaaa
     6901 gttccatttc ctttgtttat agcactttgt aaatattcaa ggccaattat ttcaatgtgt
     6961 ctttccaaat attttttact gaccaacgca tataaaataa tttccttaat actagttata
     7021 atatgagaat aataagccat acatagacgt tttttttcag agggagctag agaggattgg
     7081 aatacgcgat ctatattttt tttcgctttg cttcttttgt aaggcaataa aaaatacgca
     7141 caacgccccc accaggtcac aggaaggcaa tttaaatcac gatctaattc taacataaat
     7201 gaaaactgcc attttgctca aataccgtta ctgtttacta aattgagttt aggcactcag
     7261 tctacaatct aagcatttta aagtaaaata ctacttggat ttacaaacga ctttaagcta
     7321 acatcccttc acctgatcct tatatgaggg caaattgatt aaagaaaaac gtcttagaat
     7381 cgctatggtt gccggggaac tatctggaga cttactgggg gccggggtaa ttcgtgagct
     7441 gaaacagcat ttaacaaacg tagaattcat gggtgtcggt ggtcctcaaa tgctcaagga
     7501 aggtttccat tccctgattg atatctccga gctttctgtg atgggaatca gtgatgttct
     7561 gagacgatat cctcaactgt atttaataag agaaaggtta ttgagggaat ggacaattaa
     7621 tccaccagat gtattcattg ggattgatta ccctgacttt aatttatccg ttgaagcacg
     7681 gttaaaaaaa caacatatta aaaccattca tctggtgagc cccaaagttt gggcatggcg
     7741 acaaaaaagg gtgcatttaa taaaaaaggc agttgattta gtattaaccc tatttccatt
     7801 tgaagaagca ttttacctac aacatggggt atctgcacaa tttattggtc atcctttggc
     7861 tgatttaatc gaaataaacc caagttgttc tgctttacgt aaaaaatata actaccattc
     7921 tgatgatacc attctggcag tattaccggg tagtcgagta ggtgaaatca aatacatggg
     7981 gcccttgttt ttggaagtaa tgcaaagaat tgctatggag aggcctcatg tgcatttcat
     8041 agtaccgatt gcgtgtcagg atttgtatcc tgtttttttt aaacaactcc atgccgagta
     8101 tgactactta aaaattcagg tcattcaagg gaacgctcgt gaggccatgg ctatctctga
     8161 cgttgtttta acaaaatccg gaacagcaac acttgaggcg atgcttttaa aacgacccat
     8221 ggtggttgcc tttaaatggg gtatacttac tcatgcgatt atcgcgcctc aagtaaaagt
     8281 tccctatatt gcgttgccta atttattggc tggtaaaaaa ttaatccctg aatttgttca
     8341 ggaaaaagcg aatgttgata gcattacaga atcagttctg aatttactcg attcatccaa
     8401 tcaaaacgaa ctaattaaac aatttactga tattcatcgc acattaaggc aaaatgctaa
     8461 tgagaaagcg gctttagcta tcctcaggat tttagaggat tctttgactt aatcgccgtt
     8521 tttaaaaatc ggatttgttt ttgccaatca ctgattgaaa tggcaggata accaattacc
     8581 ctatctcctg gctgtacatt tctcaaaact ttcgaacagg tcaaaacagt tgctccctta
     8641 ccaattttca agtgtccaat caccccaact tgtcccgcca aagtgacatg ttcaccaagt
     8701 tctgtactgc cggcgatgcc aacttgagcg acgattatag agcctttacc aattttgaca
     8761 ttatgcccaa tttgcacaag gttatctaaa cgacaccaat cttcaatcac tgtattgccc
     8821 agagagcctc ggtcaatgca ggtattggca cctatttcaa catcattgcc aataatgacg
     8881 ccgccggcat gaggaatttt gtaatgaccc tccgcatcac tggcaaaacc aaaaccatcc
     8941 tggcctattc gtgctccagg ataaactact acattgctcc caataaccgc gtgcctgatg
     9001 ctcacattat cttctataat acaattatca ccaatggtta caccatctcc tatataggta
     9061 ttcacaccaa tcttacatct tttgccaatt ctggcgtgat tgccgacata ggctccatga
     9121 gctattgaac agtctgagcc aattagtgct gttgattcaa tctttgccga aggagcaatg
     9181 aagctggatg atttttctga aggataaaag gcttgagcaa tcaaagcaaa ggctttatac
     9241 gggtttgggt gcaccagtaa atacatgcta tcaggggcaa atttcacata atctgggcct
     9301 atgatacaag atcgagcagc agtatttttt aattctttga cgtatttttt ttgatgcaac
     9361 attaccaaat cattgcttgt ggcttctgaa agttttgcca aaccagaaac tgtaaaggtt
     9421 tctcctttac cttcatacag tgtagcacca gaaatcttag ctaattcgga taaacgaaat
     9481 ggtcctgcag gttttgtaaa ttggtaattt gacatacaca ctctaaaaaa ttagcgataa
     9541 aagctgcgtt tggaattttc aagaaacttg agaatataag ccacatcatt attgtctatg
     9601 aattcacgct tgagtatttc aatggcattt gtcaaaagat gaccatcatg aaaaagaatc
     9661 ttgtaggctc gttgaatttg cattcttgcg acaggactgt atccatttcg ttgcaatctc
     9721 tcaatgttaa taccaaatcg cttggctggg tttccgcaaa ccagagtgta gggcagtacg
     9781 tcttttgtaa ttctggtttg actgccaatc atactcatcc gccctaattg tacgaattgg
     9841 tgtatgccgc ataaaccgcc tatggttacc caatcgtcaa tgattacatg cccggctacg
     9901 gctaccgcat tggagatgat attgtggtta cccatctgga cgtcatgagc tatatgggca
     9961 gaagtcatta tgacattgtt gtttcctatg cgagtcactc caccaccccc agaggttcct
    10021 ctactgatcg taacgtattc gcgaataatg ttattattac cgactatgac ggtgcttttt
    10081 tccccgctga acttcagatc ctgcgggatt gctccaatga ttgctcccgt ttcaatctga
    10141 ttatactcac ctatttctgt ccaggatttt atcgatgtat gagagccaat gactgtcccc
    10201 tgtccaatgc tgacattacc ttcaataatt gaatacgggc ctataaccac atcccgtcca
    10261 attctggcat cgactgacac aatagctgtt ggatggatct ggtgggtaag agtatataaa
    10321 ttattagcca catgaacatg atctaactga ctcatttgca tggtttacct cctttacaac
    10381 ataaattaaa tgctaataac aatatactct gtgctgtttt taataacttt agataaaata
    10441 atttggttta taaagcgtat gcactataag acatgacttg gattaactcc ataagtcatg
    10501 tcgggttaca ttatgcttat gctaaacaat ataacttgct tttataaaca cattacgcac
    10561 agataggcgc tcagtgatac ttacgctatt agtggcattt gccaagctat aattttcccc
    10621 attattcatt gaccagtatt gctcagagcc aattccacct tcaacaccaa tcacaccacc
    10681 attattcagg ttgaagtggg aagcaaccac cagattagcc cccagaaccg gagaaagcca
    10741 tactgaggaa ggatgattaa gaatataagc ataacggcca ccagtattcg gaaagccaaa
    10801 aatcggttcg gggcctatgg tttcgaagga ttcattccag ttacttttct tgcttccagc
    10861 caatacagat aatgaaatat ctccaccaac agtaataaac cgattaacat cccaatagag
    10921 tcctccacct gctttaggac caataccctg gtatttcgca tgaaaattga tatagtcact
    10981 agtgggttgt aaagaagcta gattgaaagc cattatggct tctccattat atcgagcagt
    11041 aaatcccttt tctatttggg tagctttcgc tccataatag cgagtgtagc ggagctgttc
    11101 tacaaatttg ctccggacat ttctgtgagt aatcagatct gccgtttgat aatcataatt
    11161 agaatttacc gcagcaacag caggcccaac aaactcttgc cccgaaaaat cctgtacaag
    11221 tatactcagc ctgttatcca aacgatcatc agcatttgaa ttagcgacta tactggagcc
    11281 atgatttttt atgtttgtgt aagtcaacac caaatcataa tctaattgag gtccaaacag
    11341 atagcccaca cttaatgagt agccccagcg agaatccaca ttaaacaaac ctgaactaat
    11401 taacccggag gcctttaaga catcagcgta ttctatttta tctaatacac tgtcgcttaa
    11461 tgagccataa actgctgagg cactgaaaat aaatctctta ctatcctgtt gtccctcacc
    11521 cattgagcct ccgaaactta atgaggtgat taaaggtaaa ctcaccaaca gaatatttct
    11581 cttcatattc attatacatc tactccttga taattaacgc tagcccccta taaccagctg
    11641 attataagta acacctataa atatatccag ctcaaaataa tcaaaacaat caaaatttgt
    11701 taaatttata cctgcgataa a
//
LOCUS       DAXLDT010000097        11638 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXLDT010000097 DAXLDT010000000
VERSION     DAXLDT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.097"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(66..1919)
                     /locus_tag="ACF2SP_002092"
     CDS             complement(66..1919)
                     /locus_tag="ACF2SP_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFD2359868.1"
                     /translation="MSIVSLNGASLILAGNCILDRADLQIQPQDRIALVGRNGAGKST
                     LLKILQGELVLDSGQIQRSSGLRISGLVQEVPGAEGESVYHFLVKSLGETGEVLSQFH
                     EFTKQGDMDKLAKCQQRMDNLNAWHLLPEIETMASRLGIDIHEQMSNLSGGMKRRVLL
                     GAALLARPDLLLLDEPTNHLDVEAIEWLENYLKSFSGAVMVVTHDREFLSQIATSILE
                     IDRGKLYLHHCNYETYLDRRESIRLSEQKQNDLFDKRLAEEEAWIRTGIKARRTRNEG
                     RVRALKVMREQYKARRNQLGTVKSLNLDVSRSGALVVEANKMSYAINDKPIIKDFSLL
                     MTRGDKLGIIGPNGCGKTTLVRLLLGEIKPDAGTIKLGTGLQVAYFDQLRRHLLEDQT
                     VMFNVGEGADYVTINGKQKHVASYLKEFLFSSDRFNQPVSSLSGGERNRLLLAKLFAK
                     PVNLLVMDEPTNDLDIETLELLEAVLADYPGTLILISHDRAFINQVVTSVLVYEENGK
                     FNEFVGGYDDYKKHQLTRNEQQTKSLVSKRSSPEKAKNLSFNEQRELSKLPQQIEAVE
                     KKIEALHSQMASPEFYQQDAKIISEVTQLLAKEEALLNQYFTRWEELEEKK"
     gene            complement(1980..2471)
                     /locus_tag="ACF2SP_002093"
     CDS             complement(1980..2471)
                     /locus_tag="ACF2SP_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HFD2359869.1"
                     /translation="MSDDQYARTGRLMFLFVWIIFFIGLFLFFYYYDKPESTIFVASR
                     TEYVLSADNEGHYWIKGKINEYPVEFLVDTGATLVAIPQGLAKDLKITGRYPVTIETA
                     NGKVTGFLTRLQHLSFGEFHLQNVKAVIIPENDDNTVLLGMNVLSQFHMIQQGKQLIL
                     KRQ"
     gene            complement(2474..2860)
                     /locus_tag="ACF2SP_002094"
     CDS             complement(2474..2860)
                     /locus_tag="ACF2SP_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFD2359870.1"
                     /translation="MQPINTKLAPKAIGTYSQAIRSGEIVFLSGQIPLDPETMQICSD
                     DIKLQITQVLENLSAVCEEAGGSLANIVKLNVYLTDLSHFPLINEAMSRYFSEPYPAR
                     AAIEVSALPKGAKVEMDGILVLSASA"
     gene            complement(2860..4983)
                     /gene="spoT"
                     /locus_tag="ACF2SP_002095"
     CDS             complement(2860..4983)
                     /gene="spoT"
                     /locus_tag="ACF2SP_002095"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFD2359871.1"
                     /translation="MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEP
                     YITHPVAAALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTK
                     LTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
                     ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEK
                     DLQHALSQLNIPYEQVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYR
                     ILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADN
                     GVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
                     TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAP
                     GAHPNPAWLNFVVTGKARSNIRHFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPE
                     TLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKDNKKPLAIKGTE
                     GMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQ
                     GEYWVDITVDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTH
                     LARVMRRLRANKVVMRLYRKKQGEI"
     gene            complement(5151..5354)
                     /gene="rpoZ"
                     /locus_tag="ACF2SP_002096"
     CDS             complement(5151..5354)
                     /gene="rpoZ"
                     /locus_tag="ACF2SP_002096"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFD2359872.1"
                     /translation="MARVTVEDCLEHVKNRFELVMVATKRARQIAVRGDQPMVEWEND
                     KPTVVALREIAEGYVTADILDED"
     gene            complement(5405..6034)
                     /gene="gmk"
                     /locus_tag="ACF2SP_002097"
     CDS             complement(5405..6034)
                     /gene="gmk"
                     /locus_tag="ACF2SP_002097"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFD2359873.1"
                     /translation="MVSDNSGNLYIVAAPSGGGKTSLVKKLIEMVGEIEVSVSHTTRP
                     MRPGEKEGVDYFFIDEEQFISMVNEGAFIEHAKVFNHWYGTSVAQINKRLQFGIDVVL
                     DIDWQGAEQIRHSYPDAVSVFIIPPSLDTLKERLMNRRQDKDNVISERMTKAQDELGH
                     YPEFDYLIVNDDFEKAAMELQSIVIANRLRIEKQVNKQAKLLSFLLSSQ"
     gene            complement(6040..6906)
                     /locus_tag="ACF2SP_002098"
     CDS             complement(6040..6906)
                     /locus_tag="ACF2SP_002098"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFD2359874.1"
                     /translation="MIHSMTAFSRIQKQFDIGDFCWEIRSVNHRYLDVSFRLPEPFRF
                     LEAELRSLLRDKIHRGKLECHLKYKDTSCDNRSMLINIGTINALLDLSSKLSTSHHLA
                     NDMTVSQVLSWPGVVEANPLDIEELSQIALELFQKSIDKLCEVRAGEGRSLKAYVEDR
                     LQALRQEVMKSKSIVVSMSAKTKDKLLTRLHELQMDVTEVRVEQEIALLLAKLDVSEE
                     LDRLETHIAEVSRTLNAEKVTGRRLDFLMQELNREANTLSSKSDSAELTLCAVEMKVL
                     IEQMREQIQNFE"
     gene            7208..7915
                     /gene="rph"
                     /locus_tag="ACF2SP_002099"
     CDS             7208..7915
                     /gene="rph"
                     /locus_tag="ACF2SP_002099"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFD2359875.1"
                     /translation="MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIV
                     EGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACI
                     DLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
                     AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSML
                     SLAEIGIPQLIEIQKNA"
     gene            complement(8244..9278)
                     /locus_tag="ACF2SP_002100"
     CDS             complement(8244..9278)
                     /locus_tag="ACF2SP_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFD2359876.1"
                     /translation="MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEH
                     KDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSE
                     ILSMEDLGLPTIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
                     PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAA
                     ETGHLVFGTLHTNSATKTINRIIDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGR
                     VAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTA
                     HEAALNKSLF"
     gene            9442..10134
                     /locus_tag="ACF2SP_002101"
     CDS             9442..10134
                     /locus_tag="ACF2SP_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFD2359877.1"
                     /translation="MSISQNIKKVRELITQTELSSNRQPNSVLLLAVSKEQSSELIRE
                     AFQQGIRHFGENYFQEAQEKILALKDLPICWHFIGPIQSNKTKGIAKYFNWVHSIDRN
                     KTAKLLNQFRSEQLPPINVCLQINLAGETTKSGIPPEHAIDLALEVKQLPNLQLRGLM
                     TIPPPQNNMQTQYDLFLKLNQLMESINKTLHLKMDTLSMGMSDDLVPAIKAGSTIVRI
                     GRAIFGERKGKL"
     gene            10134..10922
                     /gene="proC"
                     /locus_tag="ACF2SP_002102"
     CDS             10134..10922
                     /gene="proC"
                     /locus_tag="ACF2SP_002102"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFD2359878.1"
                     /translation="MNISFIGYGNMAKAIARNLQKQNKYSLSAASPSLSVGISKENIR
                     THYDNKEVAKDADVIILAVKPTQMCEVINEINPFISSGCLLISVAAGLTLTWFAKYCK
                     KGQAVVRTMPNTPAIVGFAATSMIANEFVNEKQKKWAEQLFSSIGITDWVKDEEDMDT
                     LTALSGSGPAYVFLFLEAMINAGVQLGLEETTAKHFAIQTFAGSIQLAQHSQLSLTEL
                     RKKVTSPGGTTAAALEILHGDFDALIYSAMKAAKQKAHELGKVE"
     gene            11266..>11638
                     /locus_tag="ACF2SP_002103"
     CDS             11266..>11638
                     /locus_tag="ACF2SP_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFD2359879.1"
                     /translation="MAGLTAVGYFLLSLLFTIVIFSLWLRIAIRFFRISAINPFSQLI
                     HTITDPLVKPLYWIIKKPLQPGQKYDWIAFGVLILVEFFKIISLSLLMYHAIIPFLFI
                     FLYILVDLIIQPCEILFFAVLI"
BASE COUNT         3551 a         2472 c         2000 g         3615 t
ORIGIN      
        1 aattttattt aaagatatct ttttattgtt tttcacttaa acttatcaat taaccaaata
       61 cttttttatt ttttctcttc cagctcttcc caacgagtaa aatattgatt taacaacgcc
      121 tcttccttgg ctagcagctg agttacctct gaaataatct tggcatcttg ctgataaaat
      181 tcaggtgagg ccatttgcga atgcagtgct tcaatttttt tctctacggc ttcaatctgt
      241 tgcggcaact tcgataactc acgttgttca ttaaaagata aatttttggc tttctctggg
      301 ctgctccgtt ttgaaaccag agatttcgtt tgctgctcat ttcgtgttaa ttgatgtttt
      361 ttataatcgt cataaccacc aacaaattca ttaaacttac cattttcttc ataaaccaac
      421 acactggtta ctacttgatt aataaacgct ctatcatgac tgattagtat tagagtacca
      481 ggataatcag ctaatacagc ttcaagaagt tccagagttt ctatatccaa atcattagtt
      541 ggttcatcca ttacgagtaa attaaccggt ttggcaaaaa gttttgccag taataatctg
      601 tttctttccc ctccagacaa ggaagatact ggttgattaa atcgatcaga ggaaaataaa
      661 aactctttca aataactggc aacatgtttt tgcttaccat taattgttac ataatcagcc
      721 ccttccccca cattaaacat gactgtttga tcttcaagta aatgtcggcg taactgatca
      781 aaataggcta cctgaagccc tgtccctaat tttatagttc cagcatcagg cttaatttct
      841 cccagcagta atcgaactaa agtagttttg ccacaaccat tggggccgat aatcccaagc
      901 ttgtctcctc ttgtcattaa taaagaaaaa tcttttataa tgggcttgtc gttaattgca
      961 taactcattt tgtttgcttc tactacaaga gcaccagaac ggcttacatc caaatttagg
     1021 gatttcactg ttcctaactg attacgacgt gctttgtact gctctctcat tacctttaag
     1081 gccctgactc tgccctcatt acgtgtgcgt cttgccttga tacctgtacg tatccaggct
     1141 tcttcttctg caagccgttt gtcaaacaaa tcattttgtt tttgttcgct aagccttatc
     1201 gattcacgcc tgtcaaggta agtttcataa ttacaatggt gcagatacaa ttttccacga
     1261 tcgatttcaa ggatgcttgt tgctatctgg cttaagaatt ccctgtcgtg agttactacc
     1321 attacagcac cagaaaagga ttttaaataa ttctctaacc attcaattgc ttcgacatcc
     1381 aagtggttgg taggttcatc cagtaacaat aaatcaggtc tggcaagcag tgccgcacct
     1441 aataatacgc gtcgtttcat accgcctgag agattagaca tctgctcatg aatatctatt
     1501 cccaatcggc ttgccatcgt ttcaatttca ggtaataaat gccatgcatt caagttgtcc
     1561 attcgctgct gacacttggc caatttatcc atatctcctt gtttcgtaaa ctcatgaaac
     1621 tgggacaata cttcccctgt ttcacctaag cttttaacaa gaaaatggta cactgactct
     1681 ccctcagcac ctgggacttc ctgaactaaa ccagaaatac gcaaaccgga tgagcgttga
     1741 atttgtccac tatcaagcac caactcacct tgtagtattt tcagcaatgt tgattttcca
     1801 gcgccattgc gtccaacaag agcaattcta tcctgtggct gaatctgtaa atctgcacgg
     1861 tccagaatac aattgccagc caagattaaa gaggcaccat taagggaaac tatactcatc
     1921 aaaactctct atttaaataa atctgtttga tctcatttta ttgaaaacat ctagtcagtt
     1981 cactgtcttt ttaatatcaa ttgtttgccc tgctgaatca tatgaaattg tgataggaca
     2041 ttcattccta ataacaccgt attatcatca ttttccggga ttatgaccgc tttgacattc
     2101 tgtaagtgaa actctccaaa tgacaaatgt tgcaaacgag ttaaaaatcc cgttaccttg
     2161 ccattggcgg tttctatggt aacaggatat cgcccggtaa tttttaaatc cttagccagg
     2221 ccttgcggta ttgctaccaa agtagcacca gtatccacca aaaactctac aggatattca
     2281 ttaatcttac cttttatcca ataatgtcct tcattatcag cgcttaatac atactcagta
     2341 cgactagcta caaaaatggt actttctggc ttatcgtaat agtaaaagaa taaaaacaac
     2401 cctataaaaa atataatcca aacaaataag aacatcagac gccctgtacg agcgtattga
     2461 tcatcactca tgattatgcg gatgcagaca aaaccagaat cccatccatt tcaaccttgg
     2521 caccctttgg taatgcggag acttcgattg ccgctcttgc aggataaggc tctgaaaaat
     2581 atcgactcat cgcttcgtta attaagggaa aatgacttaa atcagtgaga tagacgttta
     2641 atttgacaat attggccagg ctaccaccag cctcttcaca tacagctgat aaattttcca
     2701 ggacttgagt aatttgtaat ttaatatcat cgctgcagat ttgcattgtt tcaggatcta
     2761 atgggatctg ccctgaaaga aaaacgatct caccacttct tatcgcttga ctgtacgtgc
     2821 ctatcgcttt tggcgccaat tttgtattga ttggttgcat taaatctccc cttgtttttt
     2881 tcgatataac cgcataacta ctttatttgc ccttaatcga cgcatgactc gagccaaatg
     2941 ggttctgtcg cgaacactga tggaaaaagt gaccgcatta tgacgaccat ctctgggatc
     3001 cacattaata ttccctatat tagagtctgc ttctgaaata gcagttgcta aagcagccaa
     3061 tacacctctt tcgttggcta catctacagt aatgtctacc caatattcac cctgaacctg
     3121 ctcatcccaa cgcaaagcaa taaactgttc tggattacta cgagttgttt ttagtgtagt
     3181 gcagtcactg gcatgaacaa taatgcctct gccttgttga aatttaccaa ctatattatc
     3241 tccaggtatt ggttggcagc attcaccaaa atgcacaacc atcccttcag tgcctttaat
     3301 agccaaaggt tttttattgt ccttatccga ttcattggcg tcttgcgaaa ccaccaaccg
     3361 cttggcaata accattgcca tctgattacc tattcctatg gcatacaata agtcatcaac
     3421 cgttttatag tgtaaatcgc tgagcaatgc ctgtaatgtt tccggaggca ctttggcgta
     3481 atcactggat aattcagcca aggattgctc caataatcgt ttgcctaaaa caatagactc
     3541 agcgtgttgt tggcttttta gaaaatgacg aatatttgat cttgcctttc cagtcacaac
     3601 aaaattcagc caagccgggt tagggtgtgc tcccggggct gtaataatct ctacggtttg
     3661 accattggat aaaggaatgc ttaaaggaac caaacgcctg ttcactttcg ctgcaacaca
     3721 actatttccc actccagagt gtaccgtgta agcaaaatct accggagtag ctcctttcgg
     3781 caactccata atgtgccctt taggagtaaa aacatatact tcatcaggaa ataaatctat
     3841 tttaacgttt tcgataaatt caagagaact gcctgtgctc ctttgcattt ctagcaaact
     3901 ttgaacccac tctctggcac gaagttgggc ttcattgact tccaatccgg aagatttata
     3961 gatccagtgc gctgctacac cattatcagc tactttatcc atttctcttg tgcgtatttg
     4021 aacctcaaga ggaacaccaa aaggcccaaa caaagtggta tgcaatgact gatagccatt
     4081 ggctttggga attccgatat aatctttaaa tcgttgtggg acaggtttat aagtacagtg
     4141 caaagctccc aaaattctat aacaggagtc aatgtcttct gtaatgatgc gaaatgcgaa
     4201 cacatccgta atttctgtaa aggatgcttt tttctgcttc atttttctat aaatactata
     4261 taaatgcttt tgcctgccaa atacttgttc atagggaatg ttcaattgcg ataaggcgtg
     4321 ttgcaaatct ttctctatag tctgtgtcaa ctctcttcga tttccccgag atttttcaac
     4381 agcagactta attgccctgt aacgcatagg atataaagct tgaaaaccca aatcctcgag
     4441 gccgatataa atagaatgca tacccaaacg attggcaata ggggcataaa tctcgagagt
     4501 ttcaattgca attcgtctgc gcttggctgc gggcattgat cctaaagtgc gcatattatg
     4561 taggcgatca gccaatttaa cgataataac gcgaatgtcc ttaaccatag ccaaaaccat
     4621 cttgcgaaaa ttttctgctt gagcttcggc ctttgattca aatttaattt tggtaagctt
     4681 ggtgacgcca tcaacaaggg cagtgacttc ttcgccaaat tgattaacca gctcttcttt
     4741 acttatggag gtatcttcaa caacatcatg aagcagagta gccatgatag tttgatagtc
     4801 cagcctcatt cttgctagaa tgagggctgc tgctacaggg tgtgtaatat aaggctcacc
     4861 tgaacgacgc atttgtccat gatgggcttt ttcagccacc aaataagctt gataacattt
     4921 ctctatttgt gcctgctcaa ggtagcattt aagctcttca tcgagctctt taaagtagct
     4981 cacgcagtac tccctagtat ccaacatggc tatttatacc tcacattact caaaatacgg
     5041 ttttatgcct cgcccttgtt ccctttgctt tgcttaaata ccataacttg attcccaaga
     5101 ggtatatttc agaatgacta atattcccaa ggcaaagtca ataactaatt ttaatcttca
     5161 tccaaaatat ccgctgtaac ataaccttcg gcaatttccc taagtgctac tacagtaggc
     5221 ttatcatttt cccattcaac cataggttga tcaccacgaa ctgcaatttg ccttgctcgt
     5281 ttggtagcaa ccataaccag ctcaaaccgg tttttaacat gttctaaaca atcttcaact
     5341 gttactcgtg ccataaaatc cacctccagg ttattagaac aaatactagc tcaaatcttt
     5401 tattttactg cgatgacaag aggaaagaaa gcaatttggc ttgtttattt acctgttttt
     5461 caattcgaag cctattggca attactatcg actgtaattc cattgctgcc ttttcaaaat
     5521 catcattaac aatcagataa tcaaattcag gataatggcc aagttcatcc tgtgctttgg
     5581 tcattctctc actaatcaca ttatctttat cttgtcgcct gttcatcaag cgttctttta
     5641 atgtgtctaa agaaggggga atgataaata cactcactgc atccggatag gagtgtctaa
     5701 tttgctccgc tccttgccaa tcaatatcaa gaacgacgtc aattccaaat tgtaaccgtt
     5761 tgttaatttg agcaactgag gtaccatacc aatggttaaa tactttggca tgctcaataa
     5821 acgccccctc attcaccata gaaataaatt gttcctcatc aatgaaaaaa taatccactc
     5881 cttctttttc tccaggcctc ataggtcttg tcgtatgaga aaccgatact tcaatctcac
     5941 ccaccatttc aataagtttt ttcaccaaac ttgtttttcc tcctccggag ggtgctgcaa
     6001 caatatataa atttcctgaa ttatcactaa ccatatcatt tactcaaaat tctgaatttg
     6061 ttcacgcatc tgctcaatca atactttcat ttcaacagca cataaagtta actctgctga
     6121 atccgatttt gagcttaggg tattcgcctc tctatttaat tcttgcatta aaaaatccag
     6181 tcttctaccc gtcacctttt ctgcattcaa agtgcggctt acttccgcta tatgagtctc
     6241 taacctatcc agctcttcac tcacatcaag tttggctaat agcaaagcaa tttcttgctc
     6301 tacacgcacc tctgtaacgt ccatttgcaa ttcatgcaaa cgcgtaagta atttatcttt
     6361 agtcttggca ctcattgaaa ccacaatgct tttggatttc attacctctt ggcgcaaagc
     6421 ctgcaatcgg tcttcaacat atgcctttaa tgacctgcct tctccagctc tgacttcaca
     6481 caatttatcg atacttttct gaaataactc caaagcaatt tgagacaatt cctcaatatc
     6541 cagcggatta gcctccacaa cacctggcca tgaaagcact tgactaacag tcatatcatt
     6601 agccagatga tgagaggtag ataatttact gcttaaatca agtaatgcat taatcgtacc
     6661 tatatttata agcatagacc gattatcaca acttgtgtcc ttgtacttta gatggcactc
     6721 cagtttaccc cgatgtatct tatcgcgaag caaggatctt aactctgctt caagaaatcg
     6781 aaagggctcc ggcaaacgaa aagagacatc gagatagcga tggttaacag acctgatttc
     6841 ccaacaaaaa tcacctatat caaactgctt ttgtattcga gaaaaagccg tcatactatg
     6901 aatcataaag aatacctact ttttaacctt gcgcgaatat acctgaaatt aaaagaattg
     6961 cacagggatt gcccatcaat taatctctac aacttgctca tcagtccctg aaagtcaggc
     7021 cttaaaccaa acttgaaact taaaacaaac tgtttctaac gcaaagaatg gcaacagatc
     7081 gaatggtttg attaaaaata tttatttaac aaaaaagaga aaagaatgta caaatagcgc
     7141 atgtatttag ggtgcaactc aagtattaaa gagttatact tgggcatttt acagaggagt
     7201 atctctaatg cgtcccagta atcgtgaaca tgatcaactt cgtcccgtta caataacacg
     7261 taattttacc aattatgccg aaggctctgt attagtcgaa tttggtcaga caaaagtgat
     7321 ttgtaacgca tcaatcgtcg aaggagttcc ccggttttta aaaggtaaaa accaagggtg
     7381 gataacagct gaatatggca tgctcccacg tgcaactcat agccgtaccg aaagagaagc
     7441 cagtaaaggt aagcaaggcg gtagaacatt ggaaattcaa cgtctcatag ggcgctcatt
     7501 gagagcatgt atcgatttaa aggtattggg cgaaaatact attactcttg attgtgatgt
     7561 tattcaagcg gatgggggaa ccagaacagc agctataact ggcagctgtg tggctatgcg
     7621 agatgccatt cactggatgg ttcaacgtga aaaaataaag aagatgcctg cttttaatta
     7681 tgtagctgcc gtatctgtag gcatttacag gggtcaaccc gtgctggatt tggattatgc
     7741 agaagatgtt cttgctgaaa cagatatgaa tgtggttatg aatgaacaag gacattttat
     7801 tgaagttcaa ggcactgctg aagataactc ctttaaccga gaacaactga acagcatgtt
     7861 gtcattagct gaaattggta ttcctcaatt gattgaaatc caaaaaaatg cctgagaaca
     7921 atagcctggc ataaacagtt gccagctata gaaattaata cttcaaacct gaacagaaga
     7981 tctctgcagg aatgctttga gctaactgaa gagatctcat tgcaattatt agcattgcta
     8041 tcttcacagt tttttgagga gttctggaac ttgcctgtgc tcaattcctg taaatacgaa
     8101 caccgtagtg aaatctatct ggacttgcgc ccctaactct ctttgttaaa taatgtctta
     8161 aatttagtca tttagtttat ctaaactttc ttattcaaaa cattttttgt ctcggcctta
     8221 gttttctcgc gaacctagtc tatttaaaat aatgacttgt tcagggcagc ctcatgagca
     8281 gtgtgtttcg aaataatatt tttattaacc aattcagtca aatgttgatc caatgtttgc
     8341 attcctttgg cttgaccagt ttgaattgac gaatacattt gcgctatctt atcctcacga
     8401 attaaattac gaatagcccc ggtacacatc ataatttcaa gcgccgctac ccgaccacct
     8461 ttattctttt ttaataaact ttgagcaacg acagcttgta atgattcaga aagcatagag
     8521 cgtaccatcg atttttcttc agcgggaaat acatcgatta tcctgtttat tgtttttgtt
     8581 gcagaattgg tatgcaatgt tccaaaaacc aaatgcccgg tttctgctgc ggtcattgcc
     8641 aagcgtatag tttccaaatc acgtaattca cccaccaata taatatcagg atcttcacgt
     8701 agcgctgatc tcaaagcggc attaaagctc aatgtgtctt tgtgcacttc acgttgatta
     8761 accagacatt ttttgctttg atgaacaaac tcaatgggat cttcaactgt caaaatatgc
     8821 tcataacgac ttgaattaat ataatcgatg actgctgcaa gcgtggtact ttttcctgat
     8881 ccagttgggc cagtaaccag aaccagccct ctggaaaaac tggccatttc tttaaaaatt
     8941 gtaggtaatc ctaaatcttc catgctaaga atttcagagg gaatagttct gaaaacagca
     9001 gcggcgccgc gtgattgatt gaaagcatta accctgaacc gtgccagatt agctatttca
     9061 aaggaaaaat ctgtttcaaa aaactcttca tattcttttc tttgtctgtc attcattatg
     9121 tcataaataa tctttatgac gtctttatgc tctaatggag gtaaattaat tttgcgtaaa
     9181 tcaccatcaa cacgaatcat aggaggcatg ccagctgaaa tatgcaaatc tgatgacttg
     9241 tttttaactg aaaatgccaa caattccgcg atatccatac tctataatcc ctgccaattt
     9301 tttcattata tattgtagat taactcatga aatgattacc agcaatgatg gatatctttt
     9361 tgtagtacca ttacacttca tgcataatta atacttcttt ctataataaa gcgcctcatc
     9421 ttttgattag ccaggattaa tatgagtatt agtcagaaca taaaaaaagt aagagaactg
     9481 ataacccaga cagaattatc cagtaacagg caaccgaata gtgtcttatt attggccgtt
     9541 agtaaagaac aaagctccga attaatcaga gaagcattcc aacaagggat aagacacttt
     9601 ggagaaaact attttcagga ggcccaggaa aaaatactgg cattaaaaga tctgcctatc
     9661 tgttggcatt ttatagggcc tattcaaagt aataaaacca aaggaatagc gaaatatttt
     9721 aattgggtcc acagcattga ccgaaataaa acagccaaac ttcttaacca gttcagatca
     9781 gagcaactac caccgatcaa tgtctgttta caaattaatt tggccggaga aactacaaaa
     9841 tcaggcattc ctccagaaca tgccatcgac cttgctctcg aagtaaagca attgcctaat
     9901 ttgcaactaa ggggactgat gacaatccct ccgccgcaaa acaacatgca aacacagtac
     9961 gatttattcc ttaaactcaa tcaactcatg gaatccatca ataagacact tcatttaaaa
    10021 atggatactt tatcaatggg catgagtgat gatttagttc cagcaattaa agcaggttcg
    10081 actatagttc gaattggacg agcaattttt ggcgaacgaa aaggtaaatt atgatgaata
    10141 tcagttttat tggctatggg aatatggcga aagcaattgc ccgcaactta caaaagcaaa
    10201 ataaatactc tttgtcagct gcctcaccat ccttatctgt gggcattagt aaagaaaata
    10261 ttcgcactca ttacgataat aaagaagtgg ctaaagacgc agacgtcatt atattggcag
    10321 taaaacccac acaaatgtgt gaggtaatca atgagattaa cccttttatt tcatcaggtt
    10381 gccttctaat ctcagtggca gccggattga ccctgacctg gtttgccaaa tattgtaaaa
    10441 aaggacaagc ggtcgtaaga accatgccta ataccccggc tatagttgga tttgcagcaa
    10501 catcaatgat tgcaaatgaa tttgtaaacg agaaacaaaa aaaatgggca gaacaattat
    10561 tttcaagcat cgggattact gattgggtta aagatgaaga agacatggac actctgacag
    10621 ccctttcagg cagtggccct gcttacgtat ttttgtttct tgaagcgatg attaatgcag
    10681 gggtccaatt gggcctggaa gaaacgactg ctaaacactt tgccatacag acatttgcag
    10741 gatcaattca attagctcaa catagccagt taagtttgac tgaactgaga aaaaaagtaa
    10801 cttctccggg aggtacaaca gccgcagctc tggaaatcct gcatggagat ttcgatgcac
    10861 tcatttattc tgctatgaaa gctgcgaaac aaaaagctca tgaattagga aaagttgagt
    10921 aactcttttg agttaagaaa gattctatcg caagaagtac tctattgact tcttttattt
    10981 taatgtcagt caggatttac aaaaacctca aggtcaaggc aaaaatgttt ttaatgaggg
    11041 aattaagata aactaagcga ctgaattaaa aatacttttt aaggaagata ttgggaacat
    11101 tctgttagta ttaattcttg aactgttcaa gcaaacaatc attgtggaac aaggtttgct
    11161 cgcgttatga tagaaggtta ataaactgct taagcaattc atttctaaac catatgaata
    11221 aatttgctgg ctttattaac gaatacaatc taaagaggta aaacaatggc tggtttaact
    11281 gctgtcgggt attttttatt atccttatta tttactatcg ttattttcag tttgtggctt
    11341 agaattgcta tacgattttt tcgtatcagt gctattaatc cattctctca attaatccat
    11401 accattactg atcctctagt gaaacccttg tattggatta taaaaaagcc attgcaacca
    11461 ggacaaaaat acgactggat agcatttggg gttttaatcc tggtggaatt ttttaaaata
    11521 atttcactga gtttactgat gtatcacgca atcattccat ttctctttat ttttctctac
    11581 atacttgttg atttgataat tcaaccttgc gaaatattat tttttgctgt tctgattc
//
LOCUS       DAXLDT010000098        11500 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXLDT010000098 DAXLDT010000000
VERSION     DAXLDT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.098"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1020
                     /locus_tag="ACF2SP_002104"
     CDS             <1..1020
                     /locus_tag="ACF2SP_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFD2359880.1"
                     /translation="YYIEPNKYFSHNPHFFKSALSRYTQWDFIELVNKHKIAFHEKTL
                     GQLFCDNKSKDIVDMLLKECEQYGATIYLNTVIEQIQKTNDYSFKISTTKGRFHCHSV
                     VIATGGLSIPTMGASPFAYKIAEQFNIKVWPTRAGLVPFTLDVLEKDRLSVLSGIGVD
                     SLVNNERNQFREHILFTHRGLSGPAILQLSSYWYPGEKICINLLPEHNLLESLKTARA
                     EAPHKQLNSVLSIYLPKRVVEVFIPQKLGEKKLADSSNKDLETISHLLQNWVVKPNGT
                     EGYRTAEVTIGGVDCHAISSKTMEANNVPGLYFIGEALDVTGWLGGYNFQWAWSSGWA
                     AGQVV"
     gene            complement(1060..1812)
                     /locus_tag="ACF2SP_002105"
     CDS             complement(1060..1812)
                     /locus_tag="ACF2SP_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2628 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359881.1"
                     /translation="MAFIRHLLEYLLHPFFLCLLTLGICIFLLYRRRAPTFTKVMLFV
                     VLIVLLAMSTGWLPSYLTYQLESRYPIVKRVNPNVKWVVVLGGGQNIGEEMPANDLLT
                     SASIKRLIEGVRLFRTLPDAKLLLSGGGSAGEQSEALLLTELAKWFAIDSEKIVVETA
                     SINTADQALELAAIVHDQPFYLVTSAIHMPRSMALCQQQGLHPIAAPTDFTFFWSTDT
                     WVKMVIPNSYNLAYTSIALHEILGKIWALGFR"
     gene            complement(1812..2882)
                     /gene="lptG"
                     /locus_tag="ACF2SP_002106"
     CDS             complement(1812..2882)
                     /gene="lptG"
                     /locus_tag="ACF2SP_002106"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFD2359882.1"
                     /translation="MSRKILDRYIAKTVLSAIGLVTLMLVGLQIFILFVNQIDDLGRA
                     DYGIVQAGVFVLLQMPYQVYLFFPIASLLGCLVGLGVLANHSELVVMRAAGISIGQIT
                     GAVLKASLIVILLITSLGETIVPVMAHYANEYKVSALTGGQALLTPRGVWVRNGNDFV
                     SIASVTHENTLRYVNQFRFDNQHNLKLARQISEVKYVNNSWIAYDVKQTEFAEDHTKA
                     YHFDTLPWDVPVKPEILKITGTNPDEMTLHELNRYLREQQRNHQNVSNYKFAFFQRIT
                     QPLNTMVMMILAIPFIFGPLRSSTMGSKLLVGAMVGFGFHILNRFFGPVSTVFQWPPE
                     LAAFSPTLIFSLIGIYLMRRVR"
     gene            complement(2879..3958)
                     /gene="lptF"
                     /locus_tag="ACF2SP_002107"
     CDS             complement(2879..3958)
                     /gene="lptF"
                     /locus_tag="ACF2SP_002107"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFD2359883.1"
                     /translation="MIIFRYLTKEVFLTLIALTAILVLIFMSNQFIQYLNRAASGSIP
                     GFVIMKLLMLELPNLMGLLLPLGFYVALLLAYGRLYAESEMTVLRACGYGPKQLLKHS
                     FAMATVVLVVVAVVMIWASPVIAVERTKLIRSTGIKTLVQTIMPGRFHAINSGQQIFY
                     VQSMNRDHSQAEQIFMAKHSVQDNRLLWDVLWADKAFAEVNPQTGEEYIVLQNGKEYQ
                     GVPGHADYQIAEFASYKSKLPNPTVVMHDDARTAKTRDLWPLNNHNLKKAAELQWRIS
                     IPLMVLTLTLVAVPLSRVSPRSGKYSKLLPAIIIYIFYANFMFVSRDAIATGKIPWWI
                     GMWWVHAAVAILGILFIWRNQVKLA"
     gene            4162..5613
                     /locus_tag="ACF2SP_002108"
     CDS             4162..5613
                     /locus_tag="ACF2SP_002108"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFD2359884.1"
                     /translation="MNYGLTHTPSLTTSECLVLGVFSDLALPDFATAIDNEQQGLIKK
                     LCQRVPEPGNTVWQTDVEGHSLLIIQCGKKEEFSANSLQKRVGEITEALIKQRFSSAT
                     VCLPRLNQESAEWQLEQMIVQIDNLRYQLLDFKTKHAKSHKLESVIFHLPGATEKSLE
                     MAKAIVTGVEFCRDLANMPANICTPTYLGEQAISLSKQFDQISCQVIGPEEIKEMGMG
                     ALLAVAQGSDQPPRLIDIHYHGNKNSAPVILVGKGITFDSGGLSIKPANAMDEMKYDM
                     SGAASVLGVIKACALLKLPINLIGIIASAENLISGSAVKSGDIVTTMSGQTVEIINTD
                     AEGRLVLADALTYAERYNPDFVIDIATLTGAIIVALGNIATGYMTRDEQLAKSIECAA
                     NESQDKVWRMPLDEAYQDALESPLADMINAGFDRSAGSITAACFLSRFTEKYRWAHLD
                     IAGTAWISGKKRNATGRPVPLLIQLLRHVANSR"
     gene            5594..6028
                     /locus_tag="ACF2SP_002109"
     CDS             5594..6028
                     /locus_tag="ACF2SP_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFD2359885.1"
                     /translation="MSPIRVDFYLLASSLPESRWLVACRLIEKAYIKGHTIFVFCDNQ
                     QQAEYLDELLWTFKDDSFIPHNLQGEGPEPPPPVQIGYQKEPRGFTDILLNLSTNIPI
                     FYTRFKRVIELVSNVEHEKEVSRLHYKEYRYKGCALHTHSIE"
     gene            complement(6175..6492)
                     /locus_tag="ACF2SP_002110"
     CDS             complement(6175..6492)
                     /locus_tag="ACF2SP_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HFD2359886.1"
                     /translation="MFVTFSCDAYENITYFENVAKQLLLLMGHGGTIPGAIKSENVPD
                     ALHHLQQGLGKIKSENKPLEDDEDSEPDISLAKRAIPLIHLLQAAEKKDCDVLWSYSK
                     SPG"
     gene            complement(6515..7879)
                     /locus_tag="ACF2SP_002111"
     CDS             complement(6515..7879)
                     /locus_tag="ACF2SP_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HFD2359887.1"
                     /translation="MQAEVFYKSCGDRIVPVHLISQSQWDEGIENLTPMERNCFSVRQ
                     FKGKLGDYCFILNADGVIEKAFIGSGTGNQESALANAALLLPPGNYQFQDKLSRESGV
                     VWGLAQYRFDEYKKYELQPRVLIVEANDMDYILSLANSLFLVRDLINTPTCDMGPESL
                     AHVVEELAKTHKAYFKQWIGEELIKDNFPAIHAVGRASALAPRLLSLHWGNEKHPRVT
                     LIGKGVCFDSGGLDIKPSSGMRLMKKDMGGAAHVIGLAQWIMVQRLPIRLQVLIPAVE
                     NAIGPNAFRPGDVLTMRNGLTVEIENTDAEGRLILADALVKACEEKPDLIIDFATLTG
                     AARVAVGTEIAAMFSNHDELAQDIVSASNKVSDPVWRLPLFSPYEELLNSNIADMSNS
                     SSSSYAGAITAGLFLQRFVAKSIPWVHFDIMAWNTVSKPGKPEGGEAMGLRAVAEYLL
                     QTYG"
     gene            complement(7884..9455)
                     /gene="murJ"
                     /locus_tag="ACF2SP_002112"
     CDS             complement(7884..9455)
                     /gene="murJ"
                     /locus_tag="ACF2SP_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFD2359888.1"
                     /translation="MLTTDIMVPKKQSLIRSTSLVSLMTFISRIVGFARDMVLANFFG
                     AQAGMDAFFVAFRIPNFMRRLFAEGAFSQAFVPVLAEYQKTRSAEDVRTFIARISGYL
                     SSILTLVTVVGIVASPVIIFLFAPGFHHDSVRAELATQMLRITFPYLMLISLTAMAGA
                     ILNTYGYFGVPAFTPVLLNISMILAAVYLCPNLPQPVVGLAWGVLIAGIVQLLFQLPF
                     LYQRHLLIRPRVVRDDPGVNKVLKLMIPALFGVSIAQLNLMIDSIFASFLKVGSVSWL
                     YYTDRLTDFPLGVFGVAIATVILPHLSRKHTEQNINQYSRALDWGLRSILLIGIPAGL
                     GLSFFALPLIASCFAYGKFTAYDLLQTQKSLITLAMGVPAFMVVKVLASGFYARQDIS
                     TPVKVGAIAMVVNTLLCFVFVWHLAHAGLTLASALAGYVNCVILLFLLIKRRLFKPSP
                     GWLKYSMQLLIANFTIGVYLYFMSGTATYWLGFSPLMRLSLLLAHVFVTVVIYLLVLG
                     IIGLRPSHFRGQIKD"
     gene            9813..10079
                     /gene="rpsT"
                     /locus_tag="ACF2SP_002113"
     CDS             9813..10079
                     /gene="rpsT"
                     /locus_tag="ACF2SP_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFD2359889.1"
                     /translation="MANIKSAIKRARQNVKLRQHNASARSMYRTYIKNVLKAVESGDQ
                     EAARAAYTKAQPVIDKAANKGLIHKNKAARIKGRLVARLKAMAA"
     gene            complement(10140..11351)
                     /locus_tag="ACF2SP_002114"
     CDS             complement(10140..11351)
                     /locus_tag="ACF2SP_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2637 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359890.1"
                     /translation="MFLTINDLQSQIQLAKKRLYVLAKNWLDTQKEAINSKDKANWLN
                     AFQASTKEAMQEQLKATGFPLIRNAILVNQSEGELPQHSLEQSSSLIHSENFPEQFNS
                     RTVPRVIYVCHSGENNQVIKRLGQLTTLMEQLHTLEELTFEVQTNVHFRGLNLAYRMA
                     RSINKEWRDVEKGRETVEYYAERLQIAEKQYKLASDFCHECLGIYKEQLDARIMYFNE
                     SLNFFVECLAAAFSSVKDDRYWLTKFLLPSLPWHSMLNEVATWLFTTNGFTNPTIDSI
                     EVNRSHLKAITEQFAKLMMAPFCSLEKELQGKIEKVENEQKIARENVYAMSSELDIVI
                     HMQSQRKLAALLFPAQPLSMLGFFQGVAAAMGRDYFYSTFIKGNQSQEPRITDDEKPL
                     IEASNYSNGLH"
BASE COUNT         3683 a         2484 c         2220 g         3113 t
ORIGIN      
        1 tactacattg aacccaacaa atatttttcc cacaaccccc attttttcaa atcagcctta
       61 tcgcgttaca ctcaatggga ttttattgag ctggttaaca aacataaaat tgccttccat
      121 gaaaaaacat taggacagtt attttgtgat aataaatcta aagatatcgt tgatatgttg
      181 cttaaagaat gtgagcaata cggggcgaca atttatttaa atactgtgat agaacaaata
      241 caaaaaacca acgactattc cttcaagatt agcacgacaa aggggaggtt tcattgccat
      301 tctgtagtca ttgcgactgg aggactttct attcccacaa tgggcgcgag cccctttgcc
      361 tataaaattg cagagcagtt caatattaag gtatggccaa ccagggcagg ccttgtaccg
      421 tttacgttgg atgtcctcga aaaggataga ttatccgtcc tgtcgggaat aggcgttgat
      481 agcctagtca ataatgaaag aaatcaattt cgcgaacaca tcttatttac gcaccgtggc
      541 ttgagtggcc ctgctatctt gcaactctca tcctactggt atcctggtga aaagatatgc
      601 attaatttgc tgcccgaaca taacctcctg gaaagcttga aaacagcccg tgcagaagct
      661 ccgcataaac aattaaactc tgtattatcg atctacttgc ccaaacgagt tgttgaagtc
      721 tttattcctc aaaaactggg tgaaaaaaaa ctggctgaca gctctaataa agatttggaa
      781 acaatttccc atttgttgca gaactgggta gtaaaaccca atgggacaga aggctaccgt
      841 acagcagaag tgacaatagg cggagtagac tgccatgcta tttcatccaa aaccatggaa
      901 gccaacaatg taccaggcct atatttcatt ggagaagccc tggacgtgac ggggtggtta
      961 ggcgggtata atttccagtg ggcatggtct tccggttggg ctgccgggca agtagtgtaa
     1021 ataacggatt caaaaacaca attctcaagg cgcattagct taccggaatc ccaaagccca
     1081 aattttcccc aagatttcat gaagagcaat ggaagtatag gccaggttat aagaattagg
     1141 tatcaccatt ttgacccagg tatcagtaga ccaaaagaag gtaaaatcag ttggggctgc
     1201 tatggggtgc aatccttgct gctggcataa tgccatggaa cgtggcatgt gaatcgctga
     1261 ggtaaccaga taaaagggtt gatcatgtac tatggctgct aattcaagcg cctgatctgc
     1321 tgtattaata gaagcagttt caacgacaat tttttccgaa tcgattgcaa accacttggc
     1381 taattctgtc aataataaag cctctgattg ctcaccagca gatcctccgc ccgataaaag
     1441 caattttgcg tcgggcaaag ttctgaataa tcggacgcct tcaatcaatc gcttgatgct
     1501 cgctgaggtc aacagatcat ttgcaggcat ttcttcccca atattttgtc ctcctccgag
     1561 aacgaccacc catttcacat tcggattaac ccgcttcact atagggtatc gagactctaa
     1621 ttgataagtt aaataactgg gtaaccaacc cgtactcata gcaagcaaga caatcaatac
     1681 aacaaacaac ataactttag tgaaagtagg agcacgccga cgatatagta aaaaaatgca
     1741 aatacctaat gtcaaaagac ataaaaagaa aggatgcagc aagtattcaa gcaaatgtct
     1801 aatgaatgcc attatctgac tcttcgcatt aaataaattc caattaaaga aaagatgagg
     1861 gtaggactaa aggcagccaa ttcaggcggc cattggaata ctgtgctcac tgggccaaaa
     1921 aatcgattca gaatatgaaa cccgaatcca accatagcgc ctaccagtaa ttttgaaccc
     1981 atggttgacg aacgtaaggg gccaaaaata aaggggatag ctaatatcat cataaccata
     2041 gtattcaaag gctgggtaat tcgttgaaaa aaggcaaact tataatttga tacattttga
     2101 tggttgcgtt gttgctcacg taaatagcgg ttcaattcat gtaaagtcat ttcatccgga
     2161 ttggtgcccg taattttaag aatttccggt ttcactggca catcccacgg caaggtatcg
     2221 aaatgatagg ctttggtatg atcctctgca aattcagttt gtttcacatc ataagctatc
     2281 cacgaattat tgacatactt tacttcactt atctgacgag ccagttttaa attatgctgg
     2341 ttgtcaaagc ggaattgatt tacatagcgc agagtattct cgtgtgtgac tgaagcaata
     2401 gaaacaaaat cattaccatt acggacccaa acccctcttg gagtcagcaa agcctgccct
     2461 cctgttaaag cggaaacctt gtattcattc gcataatgcg ccattactgg tacaatagtt
     2521 tcacctaaag acgtgattaa caggatgact atcagagacg cctttaatac cgcccccgtt
     2581 atttggccga tggatatccc tgccgcacgc atcacaacca gttcactatg attagctaat
     2641 actcccaagc caaccaaaca cccgagcaag ctggcaatag ggaaaaacag ataaacttgg
     2701 tagggcatct gtaaaaggac aaaaacaccg gcttgtacga tcccatagtc tgctcgcccc
     2761 aaatcgtcta tctgattaac aaacaaaata aagatctgca aaccgaccag catcaaagtc
     2821 accaagccga ttgcagataa aaccgttttg gcaatataac gatcaagtat ttttctgctc
     2881 atgcaagctt cacctgattt cgccaaataa ataggatgcc tagaatagca acggccgcgt
     2941 gtacccacca catccctatc caccagggaa tcttcccggt agcaatcgca tcacgagaaa
     3001 caaacataaa attagcataa aaaatataga taatgatggc tggcaaaagt ttcgaatatt
     3061 ttccagaacg agggctgaca cgactaagtg gaacggcaac cagagtcaag gtcaaaacca
     3121 ttaaaggaat ggaaatacgc cactgtaatt cagccgcctt tttcaaatta tgattgttca
     3181 aaggccataa gtctctggtt tttgcagtgc gagcatcatc atgcataaca acagtcgggt
     3241 tgggcaattt ggatttatag cttgcaaatt ctgcaatctg gtaatcggca tgacccggga
     3301 caccttgata ttccttgcca ttttgcagca cgatgtattc ctcgcctgtt tgaggattaa
     3361 cttcagcaaa tgctttatct gcccacaaca catcccatag cagcctatta tcctgaacgc
     3421 tatgttttgc catgaatatc tgttctgcct gagagtgatc acgattcatg gattggacat
     3481 aaaaaatttg ctgaccgcta ttaatggcat gaaagcgccc aggcataatc gtttgaacta
     3541 aagttttgat tcctgtagaa cggattaact tggtgcgctc aacagcaatc acaggacttg
     3601 cccaaatcat tacgacggca acaactacta acacaacagt agccatggca aagctgtgtt
     3661 tcagtaattg cttggggcca taaccacaag ctcgcagaac agtcatttcg ctttcggcat
     3721 ataaccggcc atacgccaat aaaagagcaa cataaaaccc caggggcagt aacaggccca
     3781 ttaaatttgg caactccaac atcaacaact tcataatcac aaaaccagga attgaacctg
     3841 aagcagcgcg atttaagtat tgtatgaatt gattactcat aaaaataagg actaatatcg
     3901 cggttaaggc gattaaagtg aggaacactt ctttagtcaa ataacgaaaa ataatcacta
     3961 caatacccta tagaagacct tatcaattaa ttcaactctt tcagaataac atatgaaaga
     4021 acccatcatg ataattcaag acggcctatt taaaaatagt attgatttaa aacagatgat
     4081 taaaacagaa tttttcattc aaatgcttat ccaatttagg taaactagtt gtctttctca
     4141 agcggtgcag gaaaaaatat tatgaattat ggattaactc ataccccctc attaaccact
     4201 agcgaatgtc tggtgttagg cgttttttca gatttggcgc taccagattt tgcaactgca
     4261 attgataatg aacagcaagg gctgattaaa aaactttgcc agagagtccc tgaaccagga
     4321 aatactgtat ggcaaactga tgttgaaggg catagcctat taatcattca gtgcggcaaa
     4381 aaggaagagt ttagtgctaa ttcactccaa aagcgtgtag gagaaattac agaagcatta
     4441 attaaacagc gtttctcttc tgccaccgtg tgcttgcctc ggttaaatca ggaatccgct
     4501 gaatggcagt tagagcaaat gattgttcaa atcgataatt tacgatatca acttcttgat
     4561 tttaaaacga aacatgcaaa aagtcataaa ttggagtcgg taatattcca tctgcctgga
     4621 gctactgaaa aaagccttga aatggccaaa gccattgtta ccggagttga attttgccgt
     4681 gatttagcga atatgccggc caatatttgt actccgactt accttggtga gcaagccatt
     4741 tcattatcca aacaattcga ccaaataagc tgtcaagtga tagggccaga agaaataaaa
     4801 gaaatgggta tgggcgcatt attagccgtt gcccagggaa gtgaccaacc cccaagattg
     4861 atagacattc attatcacgg taacaaaaac tcagcaccgg tcattcttgt cgggaaaggc
     4921 attacttttg actcaggagg cttatctata aagccggcca acgccatgga tgaaatgaaa
     4981 tatgatatgt ctggcgctgc cagtgtgctg ggagttataa aagcctgtgc tttgctgaaa
     5041 ttgccaatta atcttatcgg cattatagcc agtgctgaaa atttgataag tggctccgct
     5101 gtaaaatcag gagacattgt aaccaccatg tcaggacaaa cagtcgaaat tatcaatacc
     5161 gatgcagagg gtcgattagt tctagcagac gctttgactt acgcggagcg atataatcct
     5221 gattttgtaa tcgatattgc tactttaaca ggagcgataa tcgtcgcttt gggcaatata
     5281 gccactggct atatgactag agatgagcag ctggccaaat caatcgaatg tgccgctaat
     5341 gaaagccagg ataaagtctg gcgtatgcca ctggatgagg cctatcaaga tgctttggaa
     5401 agcccgttgg ctgatatgat caacgccggc tttgatcgca gtgcaggaag tattactgcc
     5461 gcctgtttct tatcaaggtt tactgaaaaa tatcgttggg cccatttgga catcgcagga
     5521 acagcctgga tttctggaaa gaagcgtaat gcaacagggc gtcctgttcc tttattaatt
     5581 caattattac gccatgtcgc caattcgcgt tgatttttac ttattagcca gcagccttcc
     5641 agaatctcgc tggttggttg cttgtcgttt aatagaaaaa gcctatatca aaggacatac
     5701 tatttttgtt ttttgcgaca atcagcaaca agcagaatac cttgatgaat tattgtggac
     5761 tttcaaagac gacagtttta ttcctcataa tttgcaaggt gaaggaccag aacctcctcc
     5821 tcccgtacaa attggctatc aaaaagaacc cagaggcttt acagatatcc tgttgaattt
     5881 atccaccaat attccaattt tttatacccg atttaaaaga gtcattgaat tggtgagtaa
     5941 tgtagaacat gagaaagaag tgagtagatt gcactataaa gaatataggt ataaaggatg
     6001 tgcattgcac acacactcga ttgaataaat atttttctgc tcgctggatt tttaagctta
     6061 ttgtgtgtcc gctccctgat ggtcgcggct ctgtgttact ttattataat cagagccgcg
     6121 accatcaggg agcggataaa ggataaagaa gcccggagaa tcagcagtta cacttcaacc
     6181 tggtgatttt gaataagacc acagcacatc acaatccttt ttttcggcgg cttgcaataa
     6241 gtgaattaaa gggattgctc gtttagccaa actgatatca ggctcactat cctcatcgtc
     6301 ttctagcggt ttattttcgc tttttatttt tcccagccct tgttgtaaat gatgcaaagc
     6361 atctggtaca ttttcagact taatagcacc gggaattgtg cccccatgcc ccattaacaa
     6421 caataattgt tttgccacat tttcaaaata agtaatgttc tcatatgcat cacaactaaa
     6481 agtcacaaac ataactaact cccaactgaa aattctatcc gtaagtctgt aataaatact
     6541 cggcaaccgc tcgcaagccc attgcttcac caccttcggg ctttcctggc ttacttacag
     6601 tattccaggc catgatatca aaatgaaccc atggtattga ttttgccaca aatcgttgta
     6661 gaaataagcc cgcagtaata gccccagcat aggacgaaga actggaattg ctcatgtctg
     6721 caatattcga attcaataac tcttcgtatg gtgaaaataa aggcaaccgc cagacagggt
     6781 ctgagacttt attagatgcc gacactatat cctgagccag ttcatcatga ttagaaaaca
     6841 tggctgcaat ttcagttcct acagcgacac gagcagcacc tgttaaagta gcaaaatcaa
     6901 tgatcaaatc tggtttttct tcacaagcct taaccaaagc atctgccaga atcaatcgac
     6961 cctctgcatc agtgttttct atttctacgg ttaatccatt gcgcatggtt aaaacatctc
     7021 ctggcctaaa agcattcgga ccaatggcgt tttcaacagc ggggattaat acctgtaatc
     7081 gaatcggcaa acgctgaacc attatccact gggctaaacc tatgacgtgt gcggcaccgc
     7141 ccatatcttt tttcatcaaa cgcatgcctg atgaaggttt gatatcaagc ccaccactat
     7201 caaaacaaac accctttcca atcaaagtta cgcgtggatg tttctcattt ccccaatgca
     7261 aagataataa gcgaggcgct aaagcagaag ctcttccaac cgcatgaatg gcaggaaaat
     7321 tatcctttat caattcctct ccaatccatt gcttaaagta agccttgtgt gttttagcca
     7381 attcttcgac aacatgggct aacgattctg gccccatatc gcaggttggc gtgttaatga
     7441 gatcccgcac gagaaataaa gaattcgcca aggataaaat ataatccata tcatttgcct
     7501 caacaattaa aactcgaggc tgtaactcgt attttttata ctcatcaaat cgatattgcg
     7561 ctaatcccca aactacgcca gactcacgac ttaacttatc ttgaaattga tagttccctg
     7621 gaggcaataa aagtgcggca ttggccaaag cactttcctg attgccagtt cccgatccaa
     7681 taaaagcttt ttcaatcacg ccatccgcat tgaggataaa acaataatct cctaatttgc
     7741 ccttaaactg gcgtacagaa aaacaatttc gttccatggg ggttagattt tcaattcctt
     7801 cgtcccattg cgattgagaa atgagatgga caggaactat cctatcgccg caacttttgt
     7861 aaaatacttc tgcttgcatc tttttaatcc tttatctggc cacgaaagtg gctaggccta
     7921 agccctatta tacctaaaac taataaatag atgactacgg tgacgaacac atgagctaat
     7981 aacaagctca aacgcatcaa aggagagaaa ccaagccagt aagtcgctgt tccactcata
     8041 aaatataaat aaaccccaat ggtaaaattg gctatcaaca actgcatact gtatttcagc
     8101 catccgggag atggtttgaa caatctcctt ttaatcaaga gaaacagcaa aataacacaa
     8161 ttcacataac cagctaatgc tgaagccaac gtcaagccgg catgagccaa gtgccagaca
     8221 aatacgaaac ataaaagagt attaactacc atggctatgg cacccacttt tacaggggta
     8281 ctgatgtcct ggcgagcata aaaaccagaa gccaacactt ttactaccat aaaggcggga
     8341 actcccatgg caagagtaat caagcttttt tgagtttgta ataaatcata ggcagtaaat
     8401 ttaccataag caaaacaact ggcaatcaga ggcaaggcaa aaaaggacaa tcccaaaccg
     8461 gctggaattc ctattaataa aatcgaacgc agcccccaat ccaaagcgcg agaatattga
     8521 ttaatatttt gttctgtgtg ttttcgggac aggtggggca agataactgt cgcaatcgcg
     8581 accccaaaga cacccaaagg aaaatcagtt aatctgtctg tatagtataa ccaagagaca
     8641 ctccctacct ttagaaaaga agcaaaaata ctatctatca tcaagttaag ttgcgcaatt
     8701 gaaacgccaa ataaagccgg gatcattaac ttgagtactt tattgactcc tggatcatcc
     8761 cgaacaactc tcggcctgat aagcaaatgt cgttgataca aaaatggtaa ttgaaaaagc
     8821 aattgcacga taccggcaat caacacaccc catgccaaac caacaacggg ttgtggcaaa
     8881 ttagggcaca aatacaccgc agcaagtatc atactgatat taagcaaaac gggagtaaag
     8941 gctggaaccc cgaaataacc ataggtattt aatatggccc ctgccatcgc tgttaaggaa
     9001 ataagcatta aatagggaaa agtgatacga agcatctgag ttgccaattc agcccggacg
     9061 ctgtcatgat ggaaacctgg cgcgaataaa aaaataataa caggcgatgc tacaattccc
     9121 actacagtga ctagggttaa tatagaactc aaatagcctg aaatccgagc gatgaaagta
     9181 cgaacgtctt cagcagaacg cgttttttga tattcagcca ataccgggac aaaagcttgg
     9241 gaaaaagcgc cttccgcaaa aaggcgccgc ataaaatttg gtatcctgaa agcaacaaaa
     9301 aaagcatcca tgccagcttg agccccaaaa aaattggcaa gaaccatatc tcgtgcaaac
     9361 ccgactatcc ttgaaataaa tgtcattaat gaaacaaggg aagtggatcg tatcaagctt
     9421 tgttttttag gtaccataat atccgttgtc agcatgatgt tttaaataga taaaaatttt
     9481 ctttatgata tacttaaact ggcgcgagtt cgatataaaa agcatggaaa tacctcttat
     9541 atctggttaa gctggcatta tccagtaaaa cacaacacat caggatacgg gttccactgc
     9601 aaatgaagtc gagcatagta tcaaatgagt aagtgaatca cctgaaatat tggttttaca
     9661 agatttatcc ggataataaa acatttcaac atcaacctgc gtcaaaacaa atacataaat
     9721 acaaaaggtt caaagcaggt ctattgacaa attctaactc attgtgcaag atctacatct
     9781 tttgcaacat ctgattgagt ggagattttt aagtggcaaa tatcaaatca gcgattaagc
     9841 gtgcacgtca aaacgtcaaa ttgcgtcaac ataacgccag cgcgcgttcc atgtaccgca
     9901 cctacattaa aaacgtgctg aaagccgtag aatcaggaga tcaagaagct gcgcgtgctg
     9961 cttataccaa agcacagcca gtgatagaca aagccgcgaa taaaggctta attcataaaa
    10021 acaaagcagc acgtattaaa ggccgtctgg ttgcacgtct taaagctatg gctgcctaat
    10081 cgagagtttc cgtcgctcta agttcgaaac taccgaaccg gaattcccgg ttcggttccc
    10141 tagtgtagcc cgttggaata attgcttgcc tcgatgagag gtttttcatc atctgttatc
    10201 ctgggttctt ggctttgatt ccctttgata aaagttgaat aaaaataatc tctacccata
    10261 gctgcggcaa ccccctggaa aaagcctaac atacttaatg gttgagcagg gaacagcaat
    10321 gcggctaatt ttctttgtga ctgcatgtgt atcactatat ccaattccga ggacatggca
    10381 tacacatttt ctctggctat tttttgctca ttctctactt tttctatttt gccctgtaac
    10441 tctttttcca aagaacaaaa aggcgccatc atcaatttgg caaattgttc tgtaattgct
    10501 tttaaatgtg agcgattcac ttcaatgctg tcgattgtcg ggttagtaaa gccatttgta
    10561 gtaaagagcc atgtggccac ctcattaagc atgctatgcc aaggtaaaga gggcagtaaa
    10621 aattttgtta gccaatacct gtcgtctttt acagaggaaa aagcagcagc caggcactca
    10681 acaaagaaat ttaatgattc attaaagtac attatccgtg catccaattg ctccttataa
    10741 atccctaaac actcatgaca aaaatccgat gccaatttat attgtttttc tgctatctgc
    10801 agacgctccg cataatactc tacagtttca cgaccctttt ctacatcgcg ccactcctta
    10861 ttgatactgc gtgccatgcg ataagccaaa tttaagcctc taaaatgaac attagtttgg
    10921 acttcaaagg ttaattcctc caatgtatgc aattgttcca ttaaggtagt aagttggcct
    10981 aatctcttga ttacttgatt attttctcct gagtggcata cataaatcac tctgggcact
    11041 gttcttgagt tgaattgctc ggggaaattt tctgagtgga tcaaagacga ggattgctcc
    11101 agactatgct gcggaagttc accctccgat tgattgacca atattgcatt gcggatcagg
    11161 ggaaaacctg tggcttttaa ttgctcctgc attgcttctt ttgtcgaggc ttgaaatgca
    11221 ttgagccaat ttgccttatc ttttgaattg attgcttcct tttgagtatc cagccaattt
    11281 ttggctaaaa catacagtct ctttttagcc aattgaattt ggctttgtaa atcatttatg
    11341 gttaaaaaca tccttttatg ctcacttgtt tgcttgagtg accttaatct atcactctaa
    11401 aatgcatact tcaatcaaaa aaattcattc ataaccggat attttaatac aaattgttta
    11461 ttaataaaca acatggatta tgtaaaaaca aaggtcaagg
//
LOCUS       DAXLDT010000099        11298 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXLDT010000099 DAXLDT010000000
VERSION     DAXLDT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.099"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..1035
                     /locus_tag="ACF2SP_002115"
     CDS             64..1035
                     /locus_tag="ACF2SP_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HFD2359891.1"
                     /translation="MLKKTSLALLGLAASGYALAGTMGPVCTPGNVTVPCEAKRWDLG
                     VQALYLKPVYDADMAYRQVHVAPTRYDDDQGWDWGYRLEGSYHFNTGNDVTLTWIHYS
                     DFANPSGLVGRIDIPPLPPALRFPRLFSAIYKNRFEQVNLVMGQHADFGLVKDMRFYG
                     GLQYANIEIDNTNYYFGAFPQLGGVNLFDNTDYKGVGPVLGIDYAYALTNSLSVTANG
                     AASVLYGKGKYNIGYVALPTGSSGAVVRSIYANKRTIVPSLEAKLGLNYAYNMAQGVL
                     NLEGGYQVMNYFNALHTQFLQNMANPVTNSDYGLYGPYIGAKYIGNA"
     gene            1338..2930
                     /locus_tag="ACF2SP_002116"
     CDS             1338..2930
                     /locus_tag="ACF2SP_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFD2359892.1"
                     /translation="MKFKKIILALACLSSPLYADQDQQLKSEIQRLQHQAEDLQAQLN
                     RLQKQLANHKSSQQKHEQQAAAKPAEPKSKPTTKSGAAIEEKYHSSKVEVHAPDAHPE
                     SISFYPTALIADNRVVTYIAGTPVVSSPFLGDRPAFDGSDYIVNISSINRDVRLMQQR
                     RRLYRAYQKIGYPIPNMPIISLSGKTEPAATFNNPFRSTNTDGDITLGSSELDIAAAL
                     NENVEAYIAIAYDESPPAIGPRVNNSAFNLNMGFVNIGNLDKSPLYFTAGQVYVPFGR
                     YSSAMVSAPVTMNLARTKTRPVIFGYKSQADTGPFAAFYGYRSDTTLGRSGVGGVNLG
                     YIFGFDNDINGEIGGGFITSVADAGGMQSTGSNVGTTFGGFGSITNGNENVRKTKAAD
                     VHGHVGYDRYNLTLEWVGAVQSFRPQDLSFNGQGARPQAAQAELGMTFMAFNRPASIG
                     VGYQWTKEALALNLPKQRYIGVFNISIWKDTVESIEYRHDIDYGLTQFANGAAPPGLV
                     NLPTLGTGKSADTVSAQIGVFF"
     gene            3139..4644
                     /locus_tag="ACF2SP_002117"
     CDS             3139..4644
                     /locus_tag="ACF2SP_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HFD2359893.1"
                     /translation="MHRDCVRQEGLHKLNNLFCKFAVLKRVGKEIIFRNRVYVSVQED
                     YGSVAIKQFPSTVNCEKKFSKLMQKSSIFILFTLLLLIGSQTSFAAKIIELNIKGPIG
                     PATADYLERGIKSAQDADLIVILIDTPGGLYDSTRNIIQLFLLSDVPIVTYVSPTGAR
                     AASAGTYLMYASTLAAMAPGTQMGAASPVSLGTGFSEGEKDEKKKSTMENKVTHDAVA
                     TIRSLAQLRGRDPDFAEKAVTEGKSITANEALSKGVVNYIAKNRDDLLSQIHGIKVSQ
                     NNKTITINTESPDIQVINPDWRTRFLSVITNPTVAYLLLLLGIYGIFFELVNPGYVLP
                     GVVGAVSMLFALYALQLLPINYAGLGLIILGILFVIAEAFTPSFGALGVGGTVSFILG
                     SIMLMNTEHLAFQIAWSAIWAMAVLNILIFVLVLGMLIKSRNQKIRHGLEILVGAKGR
                     ALGDINLEGQAVIKGEIWNVHSSSPIAANKSVKVTRASGLLLEVEEDQSVY"
     gene            4688..5443
                     /locus_tag="ACF2SP_002118"
     CDS             4688..5443
                     /locus_tag="ACF2SP_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFD2359894.1"
                     /translation="MGPFLVILLVAIGLLLVSMFKVFREYERGVVFMLGRFWRVKGPG
                     LIIIIPVIQQVVRVDLRTIVMDVPSQDVISRDNVSVRVNAVVYFRVVVPENAIIQVEN
                     YFEATSQLAQTTLRSVLGQHDLDDMLAEREQLNSDVQKILDAQTESWGIKVSNVEIKK
                     VDLDESMIRAIAKQAEAERDRRAKVIHAEGELQASEKLLQASQVLAQQPQAMQLRYLQ
                     TLATIAVNNNSTIIFPMPMEIGDILTSMVGKKK"
     gene            5682..6248
                     /gene="dcd"
                     /locus_tag="ACF2SP_002119"
     CDS             5682..6248
                     /gene="dcd"
                     /locus_tag="ACF2SP_002119"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFD2359895.1"
                     /translation="MSIKSDRWIEKMALEHGMISPFQAGQVRENQNGRIISYGVSSYG
                     YDVRCSNEFKIFTNINSAIVDPKAFDENSFVDVQSDVCIIPPNSFALARTVEYFRIPR
                     NILTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYAYEGVAQMLFL
                     EANEVCAVSYRDRNGKYQGQTGVTLPRT"
     gene            complement(6264..6575)
                     /locus_tag="ACF2SP_002120"
     CDS             complement(6264..6575)
                     /locus_tag="ACF2SP_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFD2359896.1"
                     /translation="MIKSELIEHIAARMTHLTEKQVADGINRILELMSEALIHGQRIE
                     IRGFGSFSLHYRPPRNAHNPKTGEKVVTEAKYSPHFKPGKELRERVNSSRAKFPLLDK
                     D"
     gene            complement(6828..7544)
                     /locus_tag="ACF2SP_002121"
     CDS             complement(6828..7544)
                     /locus_tag="ACF2SP_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFD2359897.1"
                     /translation="MLDFISSKMLFLCLILFLILFWLCFDKTRKELRINRWQKKLNLR
                     HHAQIFHLLYDDVNGFMISQQARKNRDAIEYVYGEIEFLPFTALLSMANIDHNTVFYD
                     LGSGTGKAVVACAMVYPVHKSIGVELFPNLHQCACERLQKLAAMEGYTESSKKISFIL
                     GDFLTVDLSEATLIFINSSTLFGATWEALNTRLNSLPQLSTVITTSKTLSSSRFKLVT
                     RAKIQMSWGVVFAFIHKKEN"
     gene            complement(7549..8274)
                     /locus_tag="ACF2SP_002122"
     CDS             complement(7549..8274)
                     /locus_tag="ACF2SP_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359898.1"
                     /translation="MEHYQEDDLARIKELLRHTGEFIAYFEFVETKMMEWREEIEQQA
                     ERVLHIAQSIQNELTSMQNLISQSGVTHFKISAEKALAQGETHLHSLERTCNQFTQIF
                     QQQQDKLNTLTENCIDRIEKHTGQATQTLTLQLSKYDVQHFHRIANESCDHVDRVAND
                     AINKSNKMLNMLQFKVGLFAMLITVLTAFVIVIYLNDEMPWEMHHQAMSERQAGKVLL
                     QAWPKLSQKEKAKILNEDGLKPG"
     gene            8860..9903
                     /locus_tag="ACF2SP_002123"
     CDS             8860..9903
                     /locus_tag="ACF2SP_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359899.1"
                     /translation="MPRARFFKAETNSSHVQQSFINVGGEQFHAAAVALIDYFKRSPR
                     INDDTLKKILERFYFHFPKYIPSQPYLTPSERMSMLINGARKSEIVECFAYVLRQLAI
                     DEIYAKPLNYRDIFAEFNRNTPKSLLRQPSTLLPASALRALSHVIGTNITLSVVENGK
                     ELRKREMYVGDNLNSAKPGITIQVQQNNKYFPAVKNKADFVYVGQLAVSPPKPVESSN
                     TSGKDTIADMVDLIASDNKKLLQTYKQWHQNFLTMLKLGEITTQRMINLYIKFLPSEK
                     GLFVDSTEFFSRLSEDSDKPVIPDYSGENKDGVNQLLASALAGWISARQVDVDQLFDH
                     LENRPSSLSMSVG"
     gene            complement(9998..10948)
                     /locus_tag="ACF2SP_002124"
     CDS             complement(9998..10948)
                     /locus_tag="ACF2SP_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HFD2359900.1"
                     /translation="MIYPNILATIGHTPVVKINRLGKDLECELYAKCEFFNPGGSVKD
                     RIGYEMVVKAEKERRIKPGDTLIEPTSGNTGIGIALAGAVLGYKVIITMPEKMSQEKQ
                     SVLERLGAIIYRTPTEAAYNDPDSHISLAKKLQAEIPNSHILDQYANPNNPNAHYFGT
                     AQEIIDDFGKDLHMVVAGVGTGGTITGIAKRLKEFNPAIKIIGADPEGSILGGGTEVK
                     SYHVEGIGYDFFPDVLDNTLIDAYIKTNDADSFRTARRLIKEEGLLIGGSCGAAMWAA
                     LQAAKSLSKGQKCLVILPDSIRNYMSKFANDEWMKEMGFL"
BASE COUNT         3341 a         2107 c         2378 g         3472 t
ORIGIN      
        1 taattaagtt ctttttgagc ccatcaccaa aatgatgggc tttttttttg aaatggagtc
       61 catatgttga aaaagacaag tttagcccta ttgggattgg ctgcaagtgg ttatgcattg
      121 gcaggaacta tgggacctgt ttgtacgcct ggtaatgtca ctgttccctg tgaagcaaaa
      181 cgttgggatt taggtgtgca ggcactttat ctaaaaccag tttatgatgc cgatatggct
      241 tatagacaag ttcacgtcgc ccccacaaga tatgatgatg atcagggttg ggattgggga
      301 taccgattag aaggttctta tcactttaat actggcaatg atgtgactct tacatggatt
      361 cactacagtg attttgcaaa tcctagtggt cttgtcgggc gaattgatat ccctccattg
      421 ccgcctgcat taaggttccc acggttattt agcgcaatat acaaaaacag atttgagcaa
      481 gtgaacctgg tgatggggca acatgctgat tttggcttgg tgaaggacat gcgattctat
      541 ggcggtttac agtacgccaa tattgaaatt gataacacga actattattt tggcgcattt
      601 cctcagttag gtggggtcaa cctgttcgac aacactgatt acaaaggagt aggacccgtt
      661 ctaggaatcg attatgccta tgccttaact aattcattga gtgtcacagc aaatggcgct
      721 gcttcagtct tatatggcaa gggtaaatat aacatcgggt atgttgcatt gcctacaggc
      781 tcttctggag cagtagtgcg atccatctat gcaaacaaaa gaacaatagt tcccagcctg
      841 gaagctaagc ttggattaaa ttatgcttac aatatggccc aaggagtatt aaatcttgag
      901 ggtggttatc aggtaatgaa ctattttaat gccctgcata cacaattcct gcaaaacatg
      961 gcaaacccag taaccaatag tgattatgga ctctatggac catatattgg tgcaaaatat
     1021 attggtaatg cctaaacgaa gtcctttact taaatgcgtt cgtcatcgat gaacgcattg
     1081 cttttaaaat cctgcttaaa aatcttgaaa aaaattcttt cacaaaaaat ataatcacgg
     1141 taatccattc taatctaagt ctaatcaact aaacacctaa tttattgatg tacaggcaat
     1201 aaactataaa tgcatacaaa taaatcaatt tattttaaat tttagcggag tgatcctata
     1261 gccagccagt aattgccgcg ctataatccc gtggctatcc gtttaggatg taaaaataat
     1321 aaacaggagt accccgcatg aagtttaaaa aaattatttt ggctctcgct tgtttatcat
     1381 cgccattata tgcggatcaa gatcaacaac tgaaaagtga aattcaacga ttacagcatc
     1441 aagcagaaga tttacaagcc caactcaacc gattacaaaa acaattagca aatcataaaa
     1501 gctctcagca aaagcacgag caacaggctg ccgcaaagcc tgcagaacca aagagtaagc
     1561 cgacgactaa atcaggggct gcaatagaag aaaaatatca ctcatccaaa gtcgaagtcc
     1621 atgcacctga tgcccatcca gagtcaataa gcttttatcc aacagcatta atagccgata
     1681 acagagtggt cacctatata gcaggaactc ctgtcgtaag ctcaccattc ctcggagacc
     1741 ggcctgcgtt tgatggttcc gattatatag taaacatttc cagtattaac cgagatgttc
     1801 gtttaatgca gcagcgacga cgcttataca gagcttatca aaaaataggc taccctatcc
     1861 ctaatatgcc aattatttca ttaagtggta aaacagagcc agctgcgaca tttaacaatc
     1921 catttcgatc aactaatacg gatggtgata ttacgctagg ctcaagtgag ttggatattg
     1981 ctgctgcatt gaatgaaaat gttgaagcct atattgcaat tgcctatgat gaaagccctc
     2041 ctgcaattgg cccacgggtt aataattcgg catttaattt gaatatggga tttgtcaaca
     2101 ttggtaacct ggataaaagc ccgctttatt ttactgcagg gcaagtctat gttccttttg
     2161 gccgctattc tagtgcgatg gttagtgcgc ctgtcacaat gaatttggca agaaccaaaa
     2221 caaggccagt cattttcggt tataagtctc aggctgatac cggtcctttc gctgcttttt
     2281 atggctacag aagcgatact acactgggtc gttcaggagt cgggggtgtt aatttaggat
     2341 acattttcgg gtttgataat gacatcaatg gcgagatagg gggcggtttc attacctctg
     2401 ttgctgatgc aggcggtatg caaagtactg gatcgaatgt ggggacaaca tttggcggtt
     2461 ttggctccat aactaacggt aatgaaaacg ttcgaaaaac aaaagctgct gatgttcatg
     2521 gccacgtggg ttatgatcgt tataatctga ctttggaatg ggttggtgcc gtacagagtt
     2581 ttaggcccca ggacttgagt tttaatggcc aaggcgccag acctcaggct gcgcaagcag
     2641 aactgggtat gacgtttatg gctttcaaca gaccggcttc cataggagtt ggttatcagt
     2701 ggacaaaaga agcattagca ctaaatctgc cgaaacaacg ttatattgga gtatttaaca
     2761 tttccatttg gaaagacaca gtggaaagta ttgagtacag gcatgatatt gattatggac
     2821 tcactcagtt cgctaatggc gcagctcctc caggattagt caaccttcca actctaggca
     2881 caggaaaatc tgccgatact gtttctgcac aaattggtgt gtttttctaa ctcatcacca
     2941 tttggtataa ttagcttgta acgaattaat ctgttacaag ctgatgtgta cccgtaccag
     3001 atggcgggtc ggtaccttac tcaattcaat tcgcctcaat aataggtaaa gatcttgaat
     3061 tgatgtgaca ctctcccctt gtttttttca acctgtgcta tcttttattt tataactacc
     3121 tcttaaaggg atgtaaaaat gcatcgggat tgcgtcaggc aagagggact gcacaaactc
     3181 aataatcttt tttgcaaatt tgctgttttg aaaagagtag ggaaagaaat aatattcagg
     3241 aatagagtat atgtgtcagt acaggaggat tacggttctg tagcaataaa acaattccct
     3301 tcaacagtaa actgcgaaaa gaaattcagc aaattaatgc aaaaaagcag tatttttata
     3361 ttatttaccc tgcttctttt gattggatcg caaacttcat tcgcagctaa aattatagaa
     3421 ttaaatataa agggaccaat tggccctgcg acagcagatt atttggaaag aggtattaaa
     3481 agcgctcaag atgctgattt gatagtgatt ctaatcgata caccaggcgg tttatatgat
     3541 tcaacacgaa atattattca gttattttta ctttctgatg tgcccatagt tacttatgta
     3601 agtcctactg gagccagggc tgccagcgct ggaacttatt taatgtatgc aagtacatta
     3661 gcggcaatgg cgcctggcac tcaaatgggg gctgcaagcc ccgtcagtct tggcacagga
     3721 ttcagtgaag gtgaaaaaga tgagaaaaaa aaatcaacca tggaaaataa ggtaactcat
     3781 gatgctgtag caacaataag gtcacttgct caactgagag gacgcgatcc cgattttgct
     3841 gaaaaggcag tgacagaagg caaatccata acagcgaatg aagccctcag caagggagtg
     3901 gtcaattata ttgcaaaaaa tagagatgat ttattatctc aaatccatgg aataaaagtc
     3961 agtcaaaata acaagacaat aactattaat accgaaagcc ctgacattca agtaatcaat
     4021 ccagattgga gaacccgctt tctatcagtc attaccaacc ctacagtggc ttacttatta
     4081 ttactacttg gaatttacgg aattttcttt gaattggtta atccaggtta tgttttacct
     4141 ggagtagtgg gcgcagtttc aatgcttttt gcgctgtatg cgctgcagct gttacctata
     4201 aattatgcag gtttgggctt gattatttta ggtattttat ttgtcatagc tgaggcattt
     4261 accccaagtt ttggggcgtt aggtgtagga ggaactgttt cttttatttt aggttccatt
     4321 atgctgatga acacggagca cctggctttt cagatagcgt ggtcagcaat ttgggctatg
     4381 gcagttctta atatattgat ttttgttttg gttctgggga tgttaattaa atctcgaaac
     4441 caaaaaatca gacatggctt ggaaattttg gtgggggcaa aaggcagggc gttaggagac
     4501 attaatctgg aaggtcaggc tgtaattaag ggagagattt ggaatgtcca ttcaagctcg
     4561 ccaatcgcgg caaataaaag tgttaaagtc acgcgagcat caggattatt gttggaagtg
     4621 gaggaagatc agtcggttta ttagttggtt attccatgtt gaatgaaaaa ggattaagga
     4681 gaatgagatg ggaccatttc tggttatatt actggtagcg ataggtttgc tactggtttc
     4741 aatgttcaag gtatttcggg aatatgaacg tggagtagtt tttatgctgg gtcggttttg
     4801 gcgtgttaaa ggtccgggat tgataatcat tatcccagtt attcaacaag tggtgcgtgt
     4861 tgatttgcga accatagtta tggatgtacc cagtcaagat gttatctcga gagataacgt
     4921 ttcagtgcga gtcaatgctg tggtgtattt tcgtgttgtc gttcctgaaa acgcaatcat
     4981 ccaggttgaa aattattttg aggcaaccag ccaattggcg caaaccacat tgcggtcagt
     5041 attgggtcag catgatttgg atgatatgtt ggctgaacgc gagcaactga atagtgatgt
     5101 gcaaaaaatt cttgatgccc agacagagag ctggggaatc aaggtttcaa atgttgaaat
     5161 caagaaggtg gatttggatg aaagtatgat aagggcaata gccaaacagg ctgaagcgga
     5221 aagagacagg agagcgaaag taatccatgc ggagggagag ttgcaagctt cggaaaaatt
     5281 attgcaagcc tctcaagtgc tcgcccagca accccaggca atgcagctaa gatatttgca
     5341 aactttagcc actatagcgg ttaataacaa ttcaacgata attttcccta tgccaatgga
     5401 gataggtgat atattaactt ccatggttgg taagaaaaaa tagaccttga acagtggcat
     5461 aagccccaaa tatacctaag tgtatagagt gctgccattg gatatgagaa catggcgata
     5521 agatttttta ttccgcgtca gcttgtggta tcagtatttt tttaaaatga gaactttcat
     5581 cgaagtttca atcattttat tctgtataat ttcccaaaac aaactttcgt ttcattgact
     5641 tttgtgatag aaatctatct ttttaagaaa agaggaatat cgtgtcaatc aaatcagaca
     5701 gatggataga gaaaatggcc cttgagcatg ggatgatttc tccatttcag gccggtcaag
     5761 tgcgagaaaa ccaaaatggt aggattattt cttatggcgt ctccagttat gggtatgatg
     5821 ttcgctgttc gaatgaattc aagattttta cgaatattaa ttcagcgatt gttgatccca
     5881 aggcgtttga tgaaaatagc tttgtagatg ttcagtctga tgtctgtatt attcccccca
     5941 actcgtttgc cttggcaaga actgtcgaat attttcgtat accccgcaat atattaacga
     6001 tatgcttagg taaatccact tacgctcgtt gcgggattat agtgaatgtt actcctctgg
     6061 aaccggaatg ggaggggcat gttactctcg agttttccaa tacgaccact ttgccagcca
     6121 agatttatgc gtatgaggga gttgctcaaa tgctgttttt agaggctaat gaggtttgtg
     6181 ctgtttctta tcgtgatcga aacggaaagt accagggaca aaccggggtt acactaccac
     6241 gcacttagga tgaacaataa aatttaatct ttatccaaaa gtgggaactt ggctcgcgac
     6301 gaattgaccc gctctcgtaa ttcctttcca ggtttaaagt gaggactgta ttttgcctct
     6361 gttactactt tttcacctgt tttaggatta tgagcatttc gcggtggtcg gtaatgaaga
     6421 gaaaaactgc caaagccacg aatttctatt ctttggccat ggataagtgc ttcactcatt
     6481 aattcaagta ttctgttgat gccatcggct acttgctttt cagtaagatg cgtcattcga
     6541 gcagcgatgt gttcaatgag ttcggattta atcatatact cacctcgttt tgtcgcgtat
     6601 ttaatagacc gattgcatcc tctattattt tagcttgtct gcctatgctt ctattcgctt
     6661 tgtatctcga agcacgggcg gccgatacta aaataaactc cacgagctga gcaactggtc
     6721 taatgtctgc taccaagaca catcaggtgc tgacaatttc ccatttgatt ttataagtat
     6781 agttcgggta aaaaattatt caatgttttc aagctggtta gtaaaaatta gttttctttc
     6841 ttatgaataa atgcaaaaac caccccccaa ctcatttgta ttttagcgcg agtaactaat
     6901 ttaaaacgac tggatgataa ggttttgcta gtagtaatga cggttgatag ttgtggcagg
     6961 ctattgaggc gggtgttaag ggcttcccag gttgcaccga ataaggtact tgaattaata
     7021 aatattaagg tcgcctcaga taaatcaacg gttagaaaat cacccaatat aaatgagatt
     7081 tttttgctac tctctgtata gccttccata gcagctaatt tctggagacg ttcgcaagca
     7141 cattgatgta agttagggaa caattcgaca cctatacttt tatggacagg gtataccata
     7201 gcgcaggcta caacggcctt accagttcca gagccaaggt cataaaaaac agtattgtga
     7261 tcaatattgg ccattgataa taaagcggtg aaaggtaaaa attcaatttc cccatacaca
     7321 tattctattg catctcgatt ctttcttgct tgctgggaaa tcatgaagcc attaacatca
     7381 tcgtacagga gatgaaaaat ttgagcatga tgccttaaat ttagtttttt ttgccatcgg
     7441 ttaatgcgca attcctttct tgttttgtca aaacaaagcc aaaaaagaat aagaaacaga
     7501 atgaggcata aaaaaagcat ttttgacgat atgaaatcaa gcacagtatt atcctggttt
     7561 cagaccatct tcattcaaga tttttgcttt ttccttttga cttaattttg gccatgcttg
     7621 aaggagaact ttaccagcct ggcgttcgct catggcttgg tgatgcattt cccaaggcat
     7681 ttcatcatta aggtaaatga ctatcacgaa agcagtgagt acggtgatta acatggcaaa
     7741 aagacctact ttgaattgaa gcatattaag cattttattg cttttgttta tggcatcgtt
     7801 tgcaactcgg tcgacatggt cacagctttc gttagcgatg cgatgaaagt gttgaacatc
     7861 gtatttagat agttgcaaag taagtgtttg agtggcttga ccagtatgct tttcgattct
     7921 atcaatgcag ttttctgtta gggtattgag cttatcttgt tgttgttgga agatttgggt
     7981 aaattgattg caagttcttt ctaaagaatg caaatgcgtt tcgccttgag ccagggcctt
     8041 ttcagcactt attttaaaat gggttactcc tgattgagaa ataaggtttt gcattgaagt
     8101 taattcattt tgtattgatt gagcgatatg tagaactcgt tcagcctgtt gctcaatttc
     8161 ctctcgccat tccatcattt tcgtttcgac aaactcaaaa taggcaataa attctccagt
     8221 atgtctcagc aactctttaa tgcgagccaa gtcatcttcc tgataatgct ccatgcctct
     8281 ctccttacta aattcatagt cagagaatct tcaaatagat ctctcgcata atctatcctt
     8341 ttcattttat tgccgcgttg caaacgtttc tatgtttgca ctttgcctaa aacaaaataa
     8401 tcagtttata caagagagct aataatatcc tttgatgcat cctgcctctt tggccgtgaa
     8461 tttagaataa tgatgttcca tattatatta tatccataac tttattttgg aaacggcaat
     8521 tttttgccta aaattctcat tcaattttca ttattagttc cataaattct caattggtac
     8581 agtatagtcc tgcctcataa agttggcatt gtttatgctt gaatgtaata tactcggcat
     8641 atactttctt tattattaaa atccactgag acgagttgtc agtttgcaga aataaacgaa
     8701 gctcaatggc attacatgaa aatgagcagc ggtttaaaaa tgatgtggtt aagagaaatg
     8761 tcccgtgtta aaaatttcag tagactgtaa aaactttgtt aatatttcct taatctggat
     8821 tgattagaat gaagatataa gttgggatag gagaacatta tgccaagagc taggttcttt
     8881 aaagcagaaa ctaatagttc gcacgtacag caaagcttta ttaatgtggg cggagagcag
     8941 tttcatgcgg ctgcagttgc tttaattgat tattttaaga gatccccacg cattaatgat
     9001 gatactttga aaaaaatact ggagcgtttc tactttcatt ttccaaaata cattcccagc
     9061 caaccttatt tgaccccctc tgaacgcatg agtatgttaa ttaatggggc acggaagtcg
     9121 gaaattgtcg aatgttttgc ttatgtatta cgccaattgg ctatagatga gatttatgca
     9181 aaacctctca attatcgtga tatctttgct gaattcaacc gtaacacgcc aaaatcattg
     9241 ttacgtcaac catctactct tctcccggcc agtgctttga gagccctttc tcatgtaatc
     9301 ggtacaaaca tcactttatc tgttgtggaa aatggcaagg aattgaggaa gcgtgaaatg
     9361 tatgttgggg ataatctgaa tagtgcaaaa cccgggataa ccattcaggt ccagcaaaat
     9421 aacaagtatt tccctgccgt gaaaaataaa gctgattttg tttatgtagg gcaattggca
     9481 gtaagccccc ccaaaccagt agaaagctcc aatacaagtg ggaaagacac tatcgctgat
     9541 atggttgatt taattgcgtc tgataataaa aaattattac agacttataa acaatggcat
     9601 cagaattttc taaccatgct taaattaggc gaaataacaa cccagagaat gatcaatctt
     9661 tatatcaagt ttttaccttc agaaaaaggc ttgtttgttg actcaacaga atttttttcc
     9721 aggttatcag aagattctga caagccagtc attcctgatt attcgggtga aaacaaggac
     9781 ggagtcaatc agttattggc ttctgcgctg gctgggtgga ttagtgccag acaggtcgat
     9841 gttgatcaat tgtttgatca tttagaaaat cgaccttctt cactatccat gtcagttggt
     9901 taaagtgtgt tggcaattcg aatcattttt tcaaagcgaa ttgtcaacag tcattttttg
     9961 atgaaatagc aataggatag tttttcatgg ctttcaatca aagaaagccc atttctttca
    10021 tccattcatc attagcaaat ttggacatgt aattacggat agagtcgggt aaaatcacca
    10081 aacatttttg tcctttgctt aaggatttgg ctgcttgcaa tgccgcccac atcgctgctc
    10141 cacatgagcc gccaattaac agtccttctt ctttaatgag gcgtctggca gtacggaaag
    10201 aatccgcgtc attggttttt atgtaggcat caatcaacgt attatccagc acatcaggga
    10261 agaaatcata acctatccct tcaacatgat aagatttgac ttctgtgccg ccaccgagaa
    10321 ttgagccttc gggatctgcc ccaatgattt tgatggcggg gttaaattct ttaaggcgtt
    10381 tggcaatacc tgtaattgtt ccgcctgtcc caacgccagc aacaaccata tgcaaatctt
    10441 ttccgaagtc gtcaataatt tcctgagctg tcccaaaata atgggcattg gggttattgg
    10501 ggttcgcgta ttgatcaagg atatgggagt tagggatttc agcttgtaat tttttagcca
    10561 gagaaatatg actgtctgga tcattatacg cggcttctgt tggcgttcga taaatgatag
    10621 ctcctaggcg ttcaagcacg gattgctttt cctggctcat tttttctggc atggtaataa
    10681 ttactttgta gcctaataca gcgcctgcca gggctatacc tatgccggtg ttcccggaag
    10741 taggctcgat taaggtatct ccaggtttaa ttctcctttc cttttctgct tttactacca
    10801 tttcatagcc aatacgatcc tttacggagc cgccaggatt aaaaaattcg catttggcat
    10861 acagctcaca ttccagatct tttccaagcc tgttaatttt tacaacggga gtatggccta
    10921 tagtggccag gatgttaggg taaatcatgg tagtcctttg tcatggtgaa atgagttgat
    10981 tataagcgga taaggtaaaa tagcaaaatg gtggtttcta caatcaagtt attttctctt
    11041 attaacgtgg tttatagatc aggatcacat aagaagcagt cgatatccag tatccgtttg
    11101 ggctgaggag atgcgtaagc atcgtctcga agccttagta ccaaacatta aattccatac
    11161 aaggcttcga gacagcataa atgcttcgtc agcccgaacg atacaagata atgacctatc
    11221 ttaatccata actcgcgttt attggaaaga aatgcaaatc atcttggcag tttatatttg
    11281 tgatatcctc cttttaac
//
LOCUS       DAXLDT010000100        11262 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXLDT010000100 DAXLDT010000000
VERSION     DAXLDT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.100"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(305..1729)
                     /gene="lpdA"
                     /locus_tag="ACF2SP_002125"
     CDS             complement(305..1729)
                     /gene="lpdA"
                     /locus_tag="ACF2SP_002125"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFD2359901.1"
                     /translation="MANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGV
                     CLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLK
                     ALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKR
                     IFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADTDLV
                     NILQKRMTKKGIKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAVGRKPN
                     GGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEV
                     IAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGR
                     EEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSE
                     TIAQSAEAFEGTITDLYLPKKKKK"
     gene            complement(1818..3452)
                     /gene="aceF"
                     /locus_tag="ACF2SP_002126"
     CDS             complement(1818..3452)
                     /gene="aceF"
                     /locus_tag="ACF2SP_002126"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFD2359902.1"
                     /translation="MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEK
                     ASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKTELEKQNSQT
                     RPEEQSVDTKATASTPDSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
                     DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILSLKTSGKTETPEIEKSQIKNISEQS
                     IKEIEKPYEELKSEPISINNLEIAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKS
                     RITKEDLQNYIKVRLNEKTTSGGFSLPSNPAIDFSKFGSIETKPLNKIKKLTGANVHR
                     SWITIPHVTQFDEADITDLEAFRKSESESTKNQDYKLTLLAFVCSVVCKALHAYPQFN
                     ASLDASGENLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREK
                     GLTPIDMSGGCFTISSLGGIGGTAFTPIVNSPEVAILGLSRSTIKPIYDNKEFKPRLM
                     LPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL"
     gene            complement(3510..6173)
                     /gene="aceE"
                     /locus_tag="ACF2SP_002127"
     CDS             complement(3510..6173)
                     /gene="aceE"
                     /locus_tag="ACF2SP_002127"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFD2359903.1"
                     /translation="MTNETNLDLDPIETREWLDALQAVLFNDGPERTAFLLQQLLNKA
                     NAEGVKLTSSINTPYRNTIKPHEEKQMPPDEGIGKRINALIRWNAVAMVLRAGKYAPE
                     LGGHIASYASASTLYETGFNYFFKGQKGENGGDLLYIQGHSAPGIYARAFLEGRLTEK
                     QLEKFRQEVEVDGLSSYPHPWLMNDFWQFPTVSMGLGPLQAIYQARFLKYLENRGLIK
                     AEGRKVWAFLGDGEMDEPESVGALSIAAREKLDNLIFVVNCNLQRLDGPVRGNGKIIQ
                     ELEGLFRGAGWNVIKVIWGGRWDPLFARDNQGWLQKRMEECLDGDYQSYKANDGSYVR
                     QHFFIQYPELKKMVENMSDEEIWRLNRGGHDPQKVYAAYARAVEHKGTPTVILAKTIK
                     GYGMGAAGEGQNITHQQKKMTIDQLKAFRDRFNIPISDDKIADIPFYKPDDDSPEIKY
                     IKKQREALGGYLPHRSTEVEQLKIPDLGEFSSITKGLGDREISTTMAFVRILSALLKN
                     KDINSRIVPIVPDECRTFGMEGLFRQIGIYSPVGQLYTPVDHEQVMYYREAVDGQILE
                     EGINEAGAFCSWIAAATSYSSNKLAMIPFYIYYSMFGFQRIGDLAWAAGDMQARGFLL
                     GGTAGRTTLAGEGLQHQDGHSHVLASTIPNCISYDPTYAYELAVIIQNGLHRMYEKQE
                     NVFYYITIMNENYSHPDMPAGVEEGIIKGMYLLKENKKKSKNHVQLMGCGTILREVIK
                     AAEMLEEDFSITSDIWSVTSFNELRKEGLAVERYNNMHPQNKPQESYVTSQLKGRRGP
                     VIASTDYMRIYADQIRPFVPNRYITLGTDGYGRSDTRTQLRHFFEVDAKFIVLAALNA
                     LVAEGSLDKAKIVDAMKRYNINQDKLDPMTH"
     gene            complement(6201..6590)
                     /locus_tag="ACF2SP_002128"
     CDS             complement(6201..6590)
                     /locus_tag="ACF2SP_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFD2359904.1"
                     /translation="MNTAFRLLFCLIILELSACATLKSKITHHKTLSQCQQTCFQQLN
                     YCKQNCTDNCRDCSTKVDHFAKENYLEYLHEVKIQGGYITRGLQSYRDPLQCRKVTCN
                     CSADFNACNQGCSGVIQKRLQPVPYCS"
     gene            complement(6629..7381)
                     /locus_tag="ACF2SP_002129"
     CDS             complement(6629..7381)
                     /locus_tag="ACF2SP_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359905.1"
                     /translation="MKLLVIVLCLLSERFLIHSVSYQRFSWFNNYCLFLKKFIDKNEY
                     FSNPWATLIAIILPIVFLTFLIYFSLQSILFGLFGLILSLFIFYYCLGPQNAFYPILK
                     KQANQTETDAIGEYFAEVNSQLFAVVFWYIIAGPIAALTYRLIALCKEINFISTQASQ
                     ITSILEWIPARITALLFLLVGNFQRGFHLFVQYVLTSPDSNDKILRGCGLQAVRINDT
                     EEVPIAAAENLVEHATIVLLVFIALFTLVAWL"
     gene            complement(7386..8558)
                     /locus_tag="ACF2SP_002130"
     CDS             complement(7386..8558)
                     /locus_tag="ACF2SP_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFD2359906.1"
                     /translation="MHDGSVFYTIFLIFAGAAFFSTLVLYTKQSLLVAYITLGAILGP
                     WGFKLVSDVTVVKQIGDIGIVFLLFLLGLHLQPQNLIHMLRKVTWIAIVSSILFAALA
                     YGIGRWFGLTVTESLILGGAMMFSSTIIGLKLLPTTILHHQHTGEVMISVLLMQDVIA
                     IVLLILLNSAHQDGNLSINDFILVIIALPALCLFSFLFERYILIKLLARFDRTQEYVF
                     LLSIGWCLGISVLAQTIGLSEDIGAFIAGVALASSPISLFIAESLKPLRDFFLVMFFF
                     SIGATFNFGFGAQVVIPAVILATLILIIKPLLFYILLARSGEKKQVAKEVGIRLGQGS
                     EFSLLVASIALSANLISEVASNLIQATTILTFIVSSYLVVLKYPTPIALSEKMRKD"
     gene            complement(8877..9698)
                     /locus_tag="ACF2SP_002131"
     CDS             complement(8877..9698)
                     /locus_tag="ACF2SP_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFD2359907.1"
                     /translation="MRKLLIVFSIFLASCQTTHDKPDLTNKRRQTTHTKNSVYDRYKY
                     KSNRYTIQQDGAPKQAKQVSFKEPVPVKEPLSRYGNPSEYYVDGRTYEVMKSSTGYKT
                     RGIASWYGTKFHKQRTSSGEPYNMYVMTAAHKTLPLPTYVKVRNLENGKVAIVKVNDR
                     GPFHANRVIDLSYAAALKLGVFPKGTAHVEIETLMGPAGQGHYYLQAGAFSSEVLAKR
                     LKEKLIHITPSPVFIEHYKQHYIVRVGPFGNKSMADNLKIKLSNNGIKGSFSVLI"
     gene            9894..11117
                     /locus_tag="ACF2SP_002132"
     CDS             9894..11117
                     /locus_tag="ACF2SP_002132"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFD2359908.1"
                     /translation="MTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTT
                     KPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQ
                     IHLNDMVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLGGSE
                     NAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWYK
                     QKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEP
                     SDSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIP
                     AGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTR
                     TKDSIRLAFKRWFGS"
BASE COUNT         3355 a         2238 c         2025 g         3644 t
ORIGIN      
        1 tttatgatga attaaatatt agcaaatgtg ttatcaaaca ttactaatcc aaaatactgc
       61 gcttaataat tctttagttt tttatttaga ccctttgcat cactgtcctt tcattttttg
      121 ctgtgctgca gatattttgt gggatgtatg taaactctac tccgcaaaaa gtttgtatct
      181 agcccaaaat aaaataatca gcccatgcaa gacgtctata tataattata tacatataat
      241 aagcatccct ttgcttgtat gagcaaagga aatgcaatga ggtataattg gctacctgta
      301 attattattt tttcttcttt ttgggcaaat acagatccgt tatagtgcct tcaaatgctt
      361 ctgctgactg agctatggtt tctgatagag tcgggtgagg gtgaatagta agagcgatgt
      421 cttcaacatc acaacacatt tcaatggcta aggcagtttc tgcaattaaa tcgccggcat
      481 taaccccaac aatacctgct cccaggatac ggttagtttc tgggcagaat agtaatttag
      541 tcatcccctc ttctcgcccc atgctgagtg cacgaccgct agcagcccat ggaaaggagg
      601 ctttctcata atttattcct ttctcttttg cttctttttc tgttagacca gcccaagcta
      661 attctggatc tgtataagca acactaggta tacattttgg ctcaaaataa tgctttttcc
      721 ctgcaatgac ttctgctgca actttccctt caggaatggc tttatgtgcg agcattggtt
      781 gtccatttac atcgccaata gcaaaaatat gaggaacatt cgttcttaat tgattatcaa
      841 ctggaataaa gccacgttca tctactttga tccctgcttt ttcagcgttg attgctccac
      901 cattaggctt tctgcctact gcaacaagta cttgctgaaa acaaagtggt ttatcagttg
      961 catgttctcc ctccattgaa acataaatac catctttttt ggcttcgaca gcagttactt
     1021 tcgtttttaa tagaaattta ataccctttt tagtcattcg tttttgcagt atattgacca
     1081 ggtcagtgtc agcattcggg attaattggt ccataaattc aacaactgtt acattaactc
     1141 ccagcgaaga gtagacagtt gccatttcca aaccaataat accaccacct aatacaagta
     1201 agtcaccttt gatatcagcg agttctaatg ctcctgtaga gctaaatatt cgtttatctt
     1261 ctggtataaa tggtaattta atggattctg agcctactgc aataatggca ttatcaaatt
     1321 caatttctac agtcccttct ttcgtttcaa caagaatttg atgggtacct gaaaacttac
     1381 ctgtcccagt aattacttct acttttcttt gtttggcaag agcttttagt cccccggtca
     1441 actttgcaac gacagaattt ttccaggcaa ccaatttttt attgtcgaat ttgggtttac
     1501 caaatgctac accttgctcc gacatttcat gggcttcatc tactacttta gcaatatgca
     1561 ataatgcttt tgagggaatg caaccaacat ttaaacaaac cccacctagt gagtcaaaac
     1621 gctcgactaa aaccaccttt ttatctaagt cggcagccct gaaagctgcg gtgtatccgc
     1681 ctggtccact acctaatacc actacatctg tcttaatttt attagccata agaagcctca
     1741 ttaaaaaata tgttttctgg ggttattggc atttcattca gaacaaggaa ataccaatag
     1801 ctctattcct ttatatgtca gagtaaaact cttctaatat cacctaaaca atcacaaaga
     1861 aagcgagtaa accgggctgc ctcagcgccg tcgattactc tatgatcgta agaaagggaa
     1921 ataggcaaca tcaatctggg cttaaactct ttattatcgt atatgggttt tatagttgat
     1981 cttgataagc ccaaaattgc aacttccggg ctatttacaa taggagtaaa agccgttccg
     2041 cctataccgc caaggctgga aattgtaaaa caacctcctg acatatcaat aggtgtcaat
     2101 cccttttctc tagctttcgt actgagtctg gacatttcct ttgcaatatc aatcacactc
     2161 aatttatcaa cattctttat tacaggaacc accagaccgt taggtgtatc tactgctata
     2221 cctatattat aatatttttt ataaattaaa ttttcaccac tggcatccag tgacgcatta
     2281 aattgcgggt aagcatggag agccttacaa actacgctac aaacaaatgc taatagagtt
     2341 aatttgtaat cttgattttt agtgctctct gattctgatt ttctaaacgc ctccaggtct
     2401 gtaatatccg cttcgtcaaa ttgagtgaca tgtggaatgg ttatccagga gcgatgtacg
     2461 tttgctccag taagcttttt aattttattt aaaggctttg tttcaattga accaaattta
     2521 ctgaaatcaa tggctggatt tgacggcaag ctgaagccgc cagaagtggt tttttcattt
     2581 aaacgtactt tgatataatt ttgcaggtct tcttttgtaa tacgagattt tctcccactt
     2641 ccctgaacca aggataaatc gactccaaac tctcttgcta atcttcttac ggctgggcct
     2701 gcagatatta agatactttt tgattcagct atctcaaggt tattaatcga tattggttcg
     2761 gattttagtt cttcatatgg cttctctatt tcctttatag attgttctga gatattttta
     2821 atctgacttt tttcgatttc aggtgtctca gtttttcctg aggtttttag acttagaata
     2881 ggagtacctt gagatacttt atcccctaat ttaattttca tttcgatgac tttacctgca
     2941 tagggcgaag gaatatccat cgttgcttta tcaccctcta atgtaattaa tgcctgatcc
     3001 ttttctactt ccatccctgg tttgactaaa atatcaatta catcaacgtc atttgcacca
     3061 ccaatatccg ggatagatat ttcaatatct ttggaatcag gtgtagatgc agttgccttt
     3121 gtatcaacag attgctcttc tggtctggtt tgcgaattct gtttttctaa ttcagtcttt
     3181 tgttcttgag aactggaaat atttgtcgtt gtgtctgact tggctttgac aatgagatcg
     3241 ccttcggata ctttgtcacc gactttaact agaatttgcg ttaccgttcc agatattgga
     3301 gaagggatat ccatgcttgc tttctcagac tcaagcgtaa tcaacggtgt atccacttcg
     3361 atctgatcac cttctttcac taaaatctca atgacatcaa cttgattagc gccaccgata
     3421 tcaggaattt taatttcact ttcttttgtc atattttcct cagcactcag ttaaaccgga
     3481 actattttct gttctgatta attgtttaac taatgggtca ttggatccaa tttgtcttga
     3541 ttaatattgt aacgcttcat ggcatcgaca atttttgctt tgtcaagact accttcagct
     3601 acaagtgcat ttaatgccgc taacactata aattttgcat caacttcaaa gaaatgacgt
     3661 agttgtgtac gggtatcact tctgccgtaa ccgtctgtac ccagggtaat atagcgatta
     3721 ggaacaaaag gcctgatctg atctgcataa atacgcatat agtcagttga ggcaataaca
     3781 ggtccacgac gtccttttaa ttgagatgtc acataacttt cctgaggttt attttggggg
     3841 tgcatgttat tgtatcgctc tacagccaaa ccttccttac gtaactcatt gaagctggtt
     3901 acactccaga tatccgatgt aattgaaaaa tcctcttcca gcatctctgc ggctttgata
     3961 acttcacgca aaatagtacc gcatcccatt agttggacat ggttttttga ttttttctta
     4021 ttttctttta agagatacat tcctttgata ataccttcct ctactcctgc aggcatatct
     4081 ggatgagagt agttttcatt cataatagta atgtaataaa acacgttttc ctgtttttca
     4141 tacatacggt gcaatccatt ttggatgata actgctaatt cataggcgta tgtaggatca
     4201 taagaaatac aattaggtat tgttgatgct aaaacatgac tatgtccatc ttggtgttgc
     4261 aatccttctc ctgccaacgt tgttcggcca gcggttccac cgagcagaaa tcctctcgct
     4321 tgcatatctc ctgccgccca tgctaaatca ccaatgcgtt ggaaaccaaa catagagtaa
     4381 taaatgtaaa atggaatcat tgccagctta tttgaactgt aagaggttgc ggcagcaatc
     4441 catgaacaaa acgccccagc ttcattaata ccttcttcaa gaatctgccc gtcaactgct
     4501 tctcggtaat acatcacttg ttcatgatct acaggagtat aaagttgacc tacgggagaa
     4561 taaattccaa tttgtctgaa taatccctcc ataccgaatg ttctgcattc atctggtact
     4621 atgggaacta ttctggaatt aatatcttta tttttgagta gagctgaaag aattcgcaca
     4681 aatgccatag tagtcgagat ttctctatct cctaatccct tggtaatact ggaaaactct
     4741 cctaaatcag gtatttttaa ctgttctact tcagttgaac ggtggggcaa ataaccgcct
     4801 aaggcttccc tttgcttttt tatatacttg atttcgggac tgtcgtcatc aggtttatag
     4861 aaaggaatat cagcaatttt gtcatcgctg attggaatat taaagcgatc tctgaaagct
     4921 tttagttgat ctatggtcat ttttttctgt tgatgagtaa tgttttgtcc ctcaccagct
     4981 gctcccatcc cgtagccctt gatggttttt gcaaggataa ctgttggagt tcccttgtgt
     5041 tcaactgctc tggcataagc tgcatatact ttttgagggt catggccacc acgatttaat
     5101 cgccaaattt cctcatcaga catattttca accattttct ttaattctgg atattgaata
     5161 aagaaatgtt gtcttacata cgatccatcg ttcgctttgt aagattgata atctccatcc
     5221 agacattctt ccatcctttt ctgtagccag ccttgattat cacgggcaaa taatggatcc
     5281 caacggcctc cccagatgac cttaattacg ttccatccag ctcctcgaaa taggccttca
     5341 agttcttgaa tgattttacc gttaccacgc actggcccat caagtctttg taaattgcaa
     5401 ttaactacaa aaatgagatt atccagcttt tctcgtgctg cgatactgag tgccccgact
     5461 gattcaggct catccatctc gccatcaccc aaaaatgccc atacttttct accctcagct
     5521 ttgatcagtc cccggttctc aagatatttt agaaagcggg cttgatagat agcttgcaaa
     5581 gggcctaagc ccatggatac agtgggaaat tgccaaaaat cgttcattag ccaagggtgt
     5641 gggtaggaag ataaaccatc tacctctact tcttgccgga atttttcaag ttgtttctct
     5701 gttaatcggc cttcaagaaa tgctctggca taaatgcctg gagccgagtg accttgaata
     5761 taaagcaagt cccctccgtt ttcacctttt tgtcccttaa aaaaatagtt aaatccagtt
     5821 tcatataacg ttgatgcaga tgcataagaa gcgatatggc cgccaagctc tggcgcgtat
     5881 ttacctgccc taagtaccat tgcaacagcg ttccatctaa ttaatgcatt gatgcgcttt
     5941 ccaattccct catcgggagg catttgtttt tcttcgtgag gttttatagt gtttctgtag
     6001 ggagtattaa ttgagctggt tagtttaacc ccctcggcgt tcgctttatt aagaagctgc
     6061 tgcaaaagga atgcagtacg ctcaggacca tcattaaaga gtacagcttg tagggcatcc
     6121 agccattcac gcgtttcaat tggatccaga tctaaatttg tttcattggt catgaaatag
     6181 ttccccaaca atcaataatt ttatgaacag tatggtacgg gttgtaatct tttctgaatt
     6241 acaccagaac aaccttgatt acatgcatta aaatccgcag agcaattaca agttactttg
     6301 cggcattgta gtggatcacg gtaagattgc aacccgcgag taatatatcc accttggatt
     6361 ttgacttcgt gaaggtattc cagataattt tctttggcga aatggtctac tttcgtagaa
     6421 caatctcggc agttgtctgt gcaattttgt ttgcagtaat taagctgctg aaaacaagtt
     6481 tgttggcatt ggcttaaggt tttatgatgg gtaattttac tttttagtgt ggcacaagct
     6541 gaaagctcca atattataag gcaaaaaagt aaacggaatg cagtgttcat gattcaagtc
     6601 ccacattaat ttattgtgaa atagaacatt atagccaagc tactaacgta aataatgcaa
     6661 taaaaaccaa caacactatt gtggcatgtt ctacaagatt ttcagcagca gctattggta
     6721 cttcttctgt atcattgata cgaactgctt gcaaaccaca tcctctcagt attttatcat
     6781 ttgagtcagg agaagttaga acatattgaa cgaatagatg aaaacctcgt tgaaaatttc
     6841 caactaacaa aaacagcaaa gcggtgattc tggctggtat ccattcgaga atgctagtaa
     6901 tttgacttgc ctgggtactt ataaaattaa tttctttaca taaagcgatt aatctgtatg
     6961 ttaaagctgc tattggccca gcgatgatgt accagaatac aacagcaaat aattggctgt
     7021 taacctcagc aaaatattct ccaattgcat ctgtttcagt ttgattagcc tgtttcttca
     7081 aaattgggta aaaagcattt tgcggcccca gacagtagta aaaaataaac aggctaagaa
     7141 ttaggccaaa taaaccaaac agaatacttt gtagtgaaaa ataaataaga aatgtgagaa
     7201 aaacaatagg caatatgata gctatcaagg tagcccaagg attagaaaaa tattcatttt
     7261 tatcgatgaa ttttttaaga aataggcagt agttattaaa ccaggaaaat cgctgatagg
     7321 acactgaatg gattaaaaac cgttcactga gcaagcataa tactattacc agtaatttca
     7381 tttgattaat ccttacgcat tttttcagat aaagctatag gtgttggata tttcaataca
     7441 acaagatacg atgaaacgat aaaagttaaa atagtagttg cctggattaa attggaggcg
     7501 acttctgaaa tcagatttgc actcaaggcg atgctcgcaa ccaataaaga aaactcactc
     7561 ccctgcccca agcgaattcc tacttctttt gccacttgtt ttttctcacc agaacgggca
     7621 agcaatatat aaaatagcaa cggcttaata atcagtatca atgttgctaa tatcaccgcc
     7681 gggataacta cctgagctcc aaatccaaaa ttaaaagtag cacctatcga aaaaaagaac
     7741 atgacaagga aaaaatctct taatggcttt aaactttcag caataaacaa tgaaattggg
     7801 cttgatgcca aagctactcc tgctataaaa gcgccaatat cttctgataa gcctattgtt
     7861 tgtgctaaaa cggatatccc caaacaccat cctatggaga gtagaaaaac gtattcctga
     7921 gttctatcaa aacgtgccag cagttttatc aggatgtatc tttcaaataa aaaagagaac
     7981 aagcacaggg ccggtaacgc gataataacc aggatgaaat cattgattga caggttccca
     8041 tcctgatgag cactattaag taatatcaaa agcactatgg ctataacgtc ctgcattagt
     8101 agtacgctaa tcatgacttc accagtatgc tggtgatgta aaatggttgt tggtaataat
     8161 ttcaagccaa taatagtact tgaaaacatc atggctccgc ccaatatcaa tgactcagtc
     8221 accgtgaggc caaaccatct gccaattccg taggcaagtg cagcaaacag aattgagctt
     8281 acgattgcaa tccaggttac ttttcttagc atatgaatca aattctgggg ctgtaaatgc
     8341 aaacctaata agaaaagcaa gaatactatt ccgatgtcac ctatctgctt gacaaccgtc
     8401 acatcggata caagtttgaa tccccaagga cctaagatag caccaagtgt tatataagca
     8461 accaataatg actgtttggt gtataatacc aaggtagaaa aaaaagcagc tccagcaaat
     8521 ataagaaata tagtgtaaaa tacagagcca tcatgcattg attgaatacc tcttaagaaa
     8581 aggtagttaa aattgaatta taaagccaat tttatccatt aatctattaa ttagctatta
     8641 attattgttt attaataata aattgttgcc tttagttaga accagggtga tatctattga
     8701 tgagaatgtc atcgatagag tcataatgtg ctggtaaaat taacacaagc tatgcacaca
     8761 ttcagaatca gtctgagtat aatgttacac ctaaaactat ttctttccca aactattctg
     8821 agtggctttt cttaattcta aagatataaa ttaaccagtt ttttccacga aacaaattaa
     8881 attaacacag aaaaggatcc ctttattccg ttatttgata attttatttt aaggttgtca
     8941 gccattgatt tgttaccaaa aggccctact cggacaatgt agtgctgttt atagtgttca
     9001 ataaacaccg gcgatggtgt aatgtgaatt agtttctctt tcaaacgctt tgccaggact
     9061 tctgaggaaa aggctcctgc ctgcaaataa taatgacctt gcccagcagg ccccattagc
     9121 gtttcaattt caacatgagc agttcctttg ggaaaaaccc ccagctttaa tgcagcagcg
     9181 tacgagagat ctatgacacg atttgcgtga aatggccctc tatcattaac cttaactatt
     9241 gcgactttgc cattttccag gtttcttact ttgacataag tcggcaaagg taatgttttg
     9301 tgggcagcag tcatgacata catgttatag ggttcaccac tggaggtacg ctgcttatga
     9361 aattttgttc cataccatga ggcaatacca cgagttttat accctgttga gcttttcatg
     9421 acttcataag tacgcccgtc aacataatat tcagaaggat taccatatct gcttaatggc
     9481 tctttgacag gcactggttc tttaaagctt acctgtttgg cttgtttggg agctccatcc
     9541 tgctgtattg tataacgatt ggacttgtat ttatatcgat cgtagacaga gtttttagtg
     9601 tgagtggttt ggcgtctttt atttgttagg tcgggtttgt catgagtagt ttggcaactt
     9661 gctaaaaaaa ttgaaaaaac aataagtagt ttacgcatat tctttgatat ttcttgttgt
     9721 tatgagttat tgctaatatt accaaaaaat aataattttc gaagtgcgat taaattaatt
     9781 cacaacttat ttagctttat aaaaaaaata ttaatatttt taatatgcca tttggccgcc
     9841 tgcaaaaggc tgtgataaga tacattcatt ttgcaactat atcaggtaat atcatgacca
     9901 gagcaacgtc ttttttattg acgctattat tttttattac tttatttgta cagtctacca
     9961 attcaatggc tgatgaagtg ctcccctcat ctgatttgca gaagccttct ccaacagtca
    10021 caacgaaacc ttttacaact ccggctgctc cattgcttaa tgccaaagct tatattttaa
    10081 ttgatgtgaa tagtggaaaa gtaattgcag aaaagaatag tgaggaaaaa ttaccacctg
    10141 ccagtttgac caaaatgatg acgctttatg tcatttctaa tgcattgaag agcgggcaaa
    10201 tccatcttaa tgatatggta agagtaagcc gtgatgcatg gcagactggt ggctctcgta
    10261 tgtttataaa agagggagat caggtctctg ttgaagacct tttaaaaggc attatagtag
    10321 actctggtaa tgacgcttgt gtggcaatgg cagagcatct tggtggttcc gaaaatgctt
    10381 ttaccgacat tatgaatcag caagctcaaa atctgggtat gaaaaatagt cattttacag
    10441 acagtactgg attacctgat cctaatctat atacaactgc taaagatttg gcgattttag
    10501 gccgagcctt aatactcgat ttccctgaat actatgactg gtataaacaa aaatggttta
    10561 catataacgg tatacgccaa cctaatagga accgtttgtt gtggcgagat agccaagttg
    10621 acggtataaa aacaggccac accaatgatg ctggatattg tctagtatct tccgcaaagc
    10681 gtaataacat gcgtttatta gctgtggttt taggggaacc cagtgattct gtgcgtgctg
    10741 atgacagtga aaaattgcta aattacggat ttagattttt tgaaacacat caattatata
    10801 aatctgggca atcaattgcc gaattaccgc tttacaaagg aacttctgat aaagttgctg
    10861 tgggtttaac agaggatcaa tttattacga tacctgccgg tcaatatgaa agattaaata
    10921 tttcaactaa aatttctaat gatttacaag ctcctataaa aaaaggcgat aaagttggcg
    10981 atattattgt gcaatttaat aacaatatcg tttcaagcaa accgctttat gctttgcaaa
    11041 cagtggagtc aggtgggttc tataccagaa caaaagattc gataaggtta gcatttaagc
    11101 gatggttcgg ttcctaggat attttcataa tgaatatcgc atttgttaac ggcaagtatt
    11161 gttgtcaatc tgaagcaaaa gtttcaatat ttgatcgagg ttttcttttc ggtgactcgg
    11221 tttatgaagt gctgcctgtt taccatgggc agccttactt tg
//
LOCUS       DAXLDT010000101        10930 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXLDT010000101 DAXLDT010000000
VERSION     DAXLDT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.101"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            44..3901
                     /locus_tag="ACF2SP_002133"
     CDS             44..3901
                     /locus_tag="ACF2SP_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2239 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359909.1"
                     /translation="MKMNEYIELYERHRYEGNNTIDSLILTLNSLKQSRESWGQDNKL
                     AKYLGFGLRNAKHPFFQPKVTQKNLHVIETVIEKLNALKESPDYQQEGETSTTRLDIL
                     GILGERFNELSKNGKTRELFNYVMFNEGSIDFSMRESTLVEPSPLEILTGNEEPYEDE
                     QTWEPPYIHQLLDSKGIPYTIFDSLETNDLNLENDLGDHVRWAYDNIYRYSYQDSHQE
                     STIARFHHGIQHVTRAANYACAFANLYRRHGNEEAQNLTETDIKLIQIALLFHDSARE
                     GDDEDLWDHESAIFLYYYLTRVLHIDAETARFVAEATANKDPSPENGYFQLIEDEYGQ
                     LFWQFAKYEEGDFPAKNIYQKIIHDCDCLDIIRARNQYDATYLDFYKDIASKDNLALE
                     EMAELVVEARGLIYSQGDSYITKKAEIKAGYENEEAHTRIIRDMQSEAYPILSTLYGK
                     LLSIEELKAMTLVNLTPFDETAGMAGENLAAALREGRVLARGITTPSAMPTPRPNKPT
                     LTDETLAEREMRKSMRSDNINIKSRKEAPNLKYKNPLRSTSILGYGSGVYPAAGMLIF
                     NPDIGQIKRISAEDFDSGRGQKNHLAHLQEKEYKESREILERELDDLHHKMKLGLFGK
                     KTQLGSNYVELLYDINKYDAIFFTNDPTIGNEIAYNDFNPAHPLSPLLQAIYLQKQYA
                     RQYEETRKAYYERYGQERGETLFLERFGPNQFLPIYEYSGLRNKLTPYDTEKLTDENI
                     VNMWVEMSSDFMRNALSHMGRDNSYDFYDELLYNMSIEDIKVLSMYKTKGNILAKVNQ
                     PADLSYNVELREKITDAIAKAKEKIIAEYEEAVFKRLQDNQLSPFSKEAFLALEYSPR
                     LRELCSDKIKDDMLRFIYSPDGVISYKLSDIAPTSLKEKTTTLDIQHCSTKERDFLCK
                     NEFLKAFILCQQLGLEGEIQFMRNKVNELAKQAIENTNAFIPENIYDLQSRLYQMINL
                     HNIMTLMDKDNHLRDNIESILLEIVSKLIDTYTNERLNLYDSYMLLKTLQNAGIPDVK
                     LKHYFRPVLNKANETLHERKPHDWGHYCSIAKLVGDEAKPYIYSWLNDYDKPFYSEIN
                     SMLETFKNAGIVFDDENLDVFKSLLEKIHYNDVAYNYVRLSYWLRVMNNLQSLASESH
                     YSESQLEILKNQFDRVIEKLIKTTKSEYEHASYYSQEQKCYRQLTSFAEEINTILASD
                     FCLPVPDSLIKEFNLTLIAFSEMDLSQTKVDKSKFHLLKQLHNKLPNPEKREHAMEKL
                     TESMDTKKQVEKDQNQNISAAGL"
     gene            4137..5345
                     /locus_tag="ACF2SP_002134"
     CDS             4137..5345
                     /locus_tag="ACF2SP_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2359910.1"
                     /translation="MSDISRGDIRVKGFTDSEMDFQLIRQLGSSSYKAASVGECLNIA
                     NTMKNEDPEEWVRQFEQLAEWQKKDGLERMVKNHVISGREQLFKASNSFRAAEYYSPP
                     SSEHHRKLGLNSADCFCTAISSMDIHFENHSIPYKNINLPCYFISPANDGEKRDTVMI
                     VSGFDGTLEEEFLMRGLAAVERGYNVIHFAGPGQMDVFRKYPDTYFEPDFEQVVKRII
                     NYFEFRQEINMAKLTLMGISFGGYFATRAASHEPRIKVLIANSPILDLHAYMCSFVKM
                     DPCEMPDSEDFSINDLPSIPNEVMPPQLKAQTEHLIRRFGQPTFKATFNYLREFTVGN
                     SLNHIKIPCLALLGSGEGGEPGKQFAEFCKITNSAQYEFSDFEGAGSHCQVGNVSYSN
                     AVVYDWLDSL"
     gene            5432..6700
                     /locus_tag="ACF2SP_002135"
     CDS             5432..6700
                     /locus_tag="ACF2SP_002135"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFD2359911.1"
                     /translation="MSPDDSKKKSKGHIVLSSHPASHTQAPLKIKWGEKNPKERGPVI
                     ASLTNIKNRNAVGTHSGSYSVYRALAVAAGLLDPEHVPDLTDTLPPVDIGPHRQWGEA
                     GKIVSLDPWGHLVASVFSEEIKAGYDIRPTIAVTKAHINIPELHIAMQKGRLKPDGKI
                     LSETGDVVVTKAAIEPVWYLPGIAERFQVSEALLRRTLFEQTAGMFPELVTRSDLDVF
                     LPPIGGLTAYFFGDVTTIHNPEIELTCRIHDECNGSDVFGSDICTCRPYLVHGIELSI
                     ESAQKGGAGLIVYNRKEGRALGEVTKFLVYNARKRQKGGDTAAKYFERTECVAGVQDM
                     RFQELMSDILHWLGITKIHRFVSMSNMKYDALLKSGINVIERVKIPDELIPPDARVEI
                     DAKRAAGYYSPEGIIDINELAKPKGRALHE"
     gene            6693..7934
                     /locus_tag="ACF2SP_002136"
     CDS             6693..7934
                     /locus_tag="ACF2SP_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HFD2359912.1"
                     /translation="MNNQEQQIQNVLNTLRDPRTIRTQSHRILNLAKQNRLEHFSLSP
                     ERMTTTASYIVDIITSRYPELDIPYHSRWRHFEMDGLPRIHNLRKQLEPISPSEWGKI
                     LYELVIISVFLDAGAGPLWRYKEALTKKEYSRSEGLALASLYLYESGAFSADPTNPFR
                     VDAERLLDFKEAELIKGFQVSSTNLLEGIPGRVSLLNRLGEIIHRKEHCFNRRRRLGE
                     FYTYVESLQDNKILPTTKLFEAVLEAFNDIWPIRLIYHGVSLGDVWIHSALKTNEAGS
                     EYIPFHKLSQWLTYSLVEPLEQTGIDVTDLDVLTGLPEYRNGGLLIDTELLKVRNNKI
                     LEQPQDPGSEPIIEWRALTVALLDELALLIRQQLNMNAESLPLAKILQGGTWEAGRQI
                     AQKKRINGTPPIEIIGDGTIF"
     gene            7947..8591
                     /gene="upp"
                     /locus_tag="ACF2SP_002137"
     CDS             7947..8591
                     /gene="upp"
                     /locus_tag="ACF2SP_002137"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFD2359913.1"
                     /translation="MDFNQVKVINHPLIQHKLTIMRKKETSTVKFRTLMHEVSMLLAY
                     EVTRDLEIEYEEIETPLATMQSPVLKGKKLVFVSILRAGNGLLDGMLQLVPTARIGHI
                     GLYRDPKTLEAVEYYFKLPEHTQDRDVIVVDPMLATGNSAIAAVKEVKALHPKSIKFL
                     CLLAAPEGISNFHGEHPDVPIFTAAIDEQLNDHGYIVPGLGDAGDRLYGTKLAH"
     gene            8897..>10930
                     /locus_tag="ACF2SP_002138"
     CDS             8897..>10930
                     /locus_tag="ACF2SP_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359914.1"
                     /translation="MLELNDKETAVIKAIHHNHKKRLESQMITRFGVYFEQSNHPEKL
                     YSWIEKIEDTRLLANHYLDTIALSRAPSTISSLEKETAGSSYYIEKAKLLIPESIRNF
                     LSDINSSNDNTLFNFVAAEVFSLALGKSITDLLFDYFNNEFLGAPENVQKEFNETLEK
                     QTELSNLIKKECERISIEIIKLFHFREMLLLGKISKSPIEPNSREEFITKAKIANQSG
                     ESVHLAIEIYFARELSRIFNSGFKSLYQVHDVEIQSEHQSPLVKWINQMSESEQVRLA
                     FTQEIQLKFMLLARDFLLEKMNQKGILARYPIIFSLFAGVIIAPIVGVTMIIMTGDTF
                     LWAIAALAVLVFALSCIATHLLINKLSVLTYQRSSDNRAHIQQSIDMINGEFLRLKKE
                     SLLKKETTCEVIENTRDFQKINQNFHLVTIGKKVARGSVSGWLREYASRYRHSKAIEI
                     DLGDEYKELITQSRIQTQELIHSINKKNSKLLIKWIHDTMTYLQDEKNQSVIEEFELI
                     PKMKEQVLEIVCQLNYIPPMLLKFYSNSREKGGLGGNESDFSHIKRLTPIDAPDKPKH
                     NPYNYLCDTALNLFKKYEPLYTSTCIFLGDPEYRQMLGIARRDAGEPLTAENINRYLD
                     NSYAFLLSLCHKIKPGLGLDPLQQSARVTDEFVLYRMLLLKQLAAISESDSKEI"
BASE COUNT         3625 a         2118 c         2140 g         3047 t
ORIGIN      
        1 attaaattgc tataatatta ttctaaagta tctttttggt acgatgaaaa tgaacgaata
       61 tattgagctt tatgaacgcc acagatatga gggaaataat acaatagatt ctttgattct
      121 aactttgaat agtttaaaac agagcaggga aagctgggga caggataaca agcttgccaa
      181 gtatttaggc tttggattgc gtaatgctaa acaccctttt tttcaaccca aagtgacgca
      241 aaaaaattta catgtaatag aaactgttat cgaaaaatta aatgctttaa aagagagtcc
      301 agattaccag caagaaggag agacatcaac aactcgcctg gatattttag ggatattagg
      361 agagcgattt aacgaattat ccaagaatgg taaaactcgt gaattattta attacgtaat
      421 gtttaatgag ggttcaattg atttctcaat gagagagagc actcttgtag aaccatcacc
      481 tttagaaatt cttactggaa atgaagagcc ttacgaagat gaacaaactt gggaacctcc
      541 ctatattcat caactactcg attcaaaagg aataccatat acaatttttg actctttaga
      601 aacaaatgat ttaaatcttg aaaatgattt gggcgatcat gtcaggtggg cttatgacaa
      661 tatttatcgt tattcctatc aggactcgca tcaggaatca actatagcta gatttcatca
      721 tggtatacaa catgttaccc gtgctgcaaa ctatgcatgt gcctttgcca atttatacag
      781 gagacatggt aatgaggaag cccaaaattt aacagaaaca gacatcaaac tcatacaaat
      841 cgctttgtta ttccatgatt cggcccgaga aggagatgat gaagatttat gggatcatga
      901 aagtgctatc tttttatact attatctgac acgcgtttta catattgatg cagaaactgc
      961 aagatttgta gctgaggcaa ctgcgaacaa ggatccgagt ccggaaaatg gctattttca
     1021 actcattgag gacgaatatg gtcaattatt ctggcagttt gcgaagtatg aagaaggaga
     1081 ttttcctgca aaaaatatct atcaaaaaat aattcatgat tgcgattgcc ttgatatcat
     1141 cagggcaaga aatcaatatg atgccaccta tcttgatttt tataaggata tagccagcaa
     1201 agacaatctc gctttagagg aaatggccga acttgtcgta gaagccagag gccttatcta
     1261 tagccagggt gattcataca ttactaaaaa agcagaaatc aaagcaggtt atgaaaatga
     1321 agaagctcat acccgcataa tcagggatat gcagtcagaa gcttatccaa tactcagcac
     1381 attgtatgga aaactgttat ctatagaaga attaaaggct atgaccttgg ttaatcttac
     1441 tcccttcgat gaaacagcag gaatggctgg agaaaatctt gctgcggcat tgagagaagg
     1501 gagagttctg gcccgtggca ttactacacc aagtgccatg cctacaccca gacccaataa
     1561 accgactcta acagatgaaa cattggccga aagagaaatg cgaaaatcca tgcgctctga
     1621 caatattaac atcaaaagca gaaaagaagc ccctaatctc aaatacaaaa accctctgcg
     1681 ctcaacctcc atactcggtt atggaagtgg agtctatcca gctgcaggca tgttgatctt
     1741 caatccggat ataggccaaa ttaagagaat ttcagctgag gattttgata gtggccgagg
     1801 tcaaaaaaac catctggccc atttacaaga aaaagaatac aaagagtctc gtgaaattct
     1861 ggaaagggaa cttgatgatc ttcatcataa aatgaaattg ggcttatttg gtaagaaaac
     1921 gcaacttggc tcaaattacg ttgaactttt atatgacatt aacaaatacg atgccatttt
     1981 ctttactaat gacccaacca taggaaatga aattgcctat aatgatttca atccagccca
     2041 tcccctctcc cctctcttgc aagcgattta tttacagaag cagtatgcga gacaatatga
     2101 agaaactcgt aaagcttatt atgaacgcta tggacaagag aggggtgaaa cactattcct
     2161 cgaacgcttt ggccctaacc aatttttacc aatttacgaa tattccggct taaggaataa
     2221 gttaacgcct tatgatacag aaaaattaac agatgagaat attgttaata tgtgggtgga
     2281 gatgtcttct gatttcatga gaaatgctct gtcccatatg ggtagagata atagctatga
     2341 tttttacgat gaacttcttt ataacatgag tattgaagac ataaaagttt taagcatgta
     2401 caaaacaaaa ggaaatatat tggcaaaagt gaatcaacca gctgatttaa gctacaatgt
     2461 tgaattaagg gagaaaatta ctgatgccat tgccaaagct aaagaaaaaa taatagcaga
     2521 atatgaagag gctgttttca aaaggcttca agacaatcaa ttatctccat tttcaaaaga
     2581 agcattttta gcactagaat atagccctcg cttgagagaa ttgtgctctg ataaaataaa
     2641 ggatgatatg ttgcgcttta tatactctcc agacggagtc atttcttaca aattatcaga
     2701 tattgcccca acgtcattaa aagaaaaaac aacgacgctt gatatccaac attgcagcac
     2761 taaggaaaga gattttttgt gcaaaaatga atttttaaaa gcctttattc tatgtcagca
     2821 attaggttta gagggagaaa tacaattcat gcgcaacaaa gtcaatgaat tggcaaaaca
     2881 agccattgag aatacaaatg ctttcatccc agagaacatc tatgatttgc aatcaagatt
     2941 atatcagatg attaacctac acaatataat gactttaatg gataaggaca atcacctaag
     3001 ggataacatt gaatcaatct tattggaaat agtttctaaa ctgatagaca cctacacaaa
     3061 tgaaaggctg aatttatacg acagctacat gttacttaag acactccaaa atgcaggcat
     3121 tccagatgtc aaattaaaac actactttag gcctgtatta aacaaagcga atgagaccct
     3181 ccatgagagg aaaccccatg attggggtca ttattgctcc attgccaagt tggtaggaga
     3241 tgaagcgaaa ccttatattt acagctggct gaatgattac gacaagccat tctattccga
     3301 gattaattcg atgcttgaga catttaagaa tgccgggatt gtttttgatg atgaaaattt
     3361 ggatgtattc aaatcattat tggaaaaaat tcattacaat gatgtggcct acaactacgt
     3421 tcgcttatca tattggctta gggtgatgaa taacctgcag tccttagctt ctgaaagcca
     3481 ttattcagaa tcccaactgg aaatcttaaa aaaccaattt gatagagtca ttgaaaagct
     3541 tatcaagact acaaaatcag agtatgaaca tgcttcctac tactcacaag aacaaaaatg
     3601 ttaccgtcaa ttaacatcgt ttgctgaaga aattaacacc attttggcct ctgatttttg
     3661 tttaccagtc cctgactcgc tgattaagga attcaatctg actctgattg cctttagtga
     3721 aatggactta agccaaacta aagtggataa atcaaagttt catcttttaa aacaacttca
     3781 taataaattg ccaaatccgg aaaaaagaga gcatgcgatg gaaaagctca ctgaatctat
     3841 ggatacaaaa aaacaagttg aaaaagatca aaatcaaaac atttcagcag ctggtctcta
     3901 gcctgataat atcagactgt gtcaattctg atttgtaccc tttgcgcgtt taaaaaagca
     3961 aatcagcaaa tcaatggatt gagcgatcat aacatcattt ggtttttaaa tttaataccg
     4021 tattttaggc attatcatga tataaggatt ttgtatttaa gattgatgtt cttggcttgt
     4081 accgttacta ttaagctcat aacactctgt aaaaacggaa ataacggaga agtaaaatga
     4141 gtgatatcag tcgtggcgat attcgggtta agggttttac tgattcagaa atggattttc
     4201 aattaatacg acaactggga tccagcagct ataaagcagc atcagtcggc gaatgtttaa
     4261 atattgcaaa caccatgaaa aatgaagacc ctgaagaatg ggtcagacag tttgaacagt
     4321 tagccgagtg gcaaaaaaag gacggtttgg aacgcatggt aaaaaaccac gtcataagcg
     4381 gtcgggagca attgttcaaa gcctccaatt catttcgcgc cgctgaatat tactcccctc
     4441 cttcctcaga acatcacaga aaattagggt tgaattcagc agattgcttt tgcacagcca
     4501 tctcaagcat ggatatccat tttgaaaacc attccattcc ttataaaaac atcaatctcc
     4561 catgctattt tatctcccct gccaatgatg gagaaaaacg agataccgtc atgatagtta
     4621 gtggctttga tggaactttg gaagaagagt tcctcatgcg tggattggct gcagtagaac
     4681 gtgggtataa tgtgattcat ttcgcaggcc ccggccaaat ggatgtcttt cgaaaatatc
     4741 cggacactta ttttgagcct gattttgagc aagtggttaa aagaatcatt aattattttg
     4801 agttcaggca agagatcaat atggccaaat tgacattgat gggcattagt tttggcggct
     4861 atttcgcaac tcgtgccgcg agccatgaac caagaatcaa ggttttaatt gccaactccc
     4921 ctattctgga tttacatgcc tatatgtgtt cctttgtaaa aatggaccct tgtgaaatgc
     4981 ctgattctga agattttagc atcaatgact taccttctat acccaatgaa gtgatgcccc
     5041 ctcaactcaa agcccaaaca gaacatttaa tcagaagatt tgggcaacct acttttaagg
     5101 caacatttaa ttatttaaga gagttcaccg taggtaattc attaaatcat attaaaatcc
     5161 cttgccttgc attattaggt agtggcgaag gaggagagcc cggaaaacaa ttcgcggagt
     5221 tttgcaaaat aacgaattct gcacaatatg aatttagtga ttttgaaggg gctggaagtc
     5281 attgtcaggt aggcaatgtt tcttattcca atgctgtggt ttatgattgg ttagacagtt
     5341 tataacaaca gtgcgcacct gcttataaag ggataaaaga taaaacaacc gatttatttg
     5401 agcaccgttg aaacaaaagg aattaagtat catgtcacca gatgattcaa agaaaaaatc
     5461 aaaaggacac attgtcttgt cttcacaccc ggcaagccat acacaagctc cattaaaaat
     5521 caaatggggt gaaaaaaacc ctaaagagag gggacctgtt attgcttctc tcaccaatat
     5581 caaaaatcga aatgccgttg gaactcactc agggtcttac tctgtttaca gggccttggc
     5641 agtagcagct ggtctgctgg atcccgaaca cgttcctgat ctaaccgaca cccttcctcc
     5701 tgttgacata gggcctcata ggcaatgggg tgaagcaggc aaaattgtct ccctcgatcc
     5761 ctgggggcat ctagtggctt ctgttttctc agaggaaata aaagccggct atgatatcag
     5821 accaaccatt gccgtcacaa aggcacatat taatatcccg gaattgcata ttgccatgca
     5881 aaaaggcaga ttaaaacctg atggaaaaat tttaagtgaa acaggggatg tcgtggtgac
     5941 taaggccgcg attgaaccag tatggtactt acctggaatt gcggagcgct tccaggtttc
     6001 agaagcattg ctcagacgca ctctttttga acaaacagct ggaatgttcc ctgaactggt
     6061 aacccgtagc gacttggatg tctttttgcc acccattggg ggattaactg cttatttctt
     6121 tggtgatgtg actaccatcc ataacccgga aatcgaatta acttgccgta tccatgatga
     6181 atgtaatggc tccgatgtct ttggttctga catatgcact tgcagaccat atttggtcca
     6241 tggaattgaa ttatctattg agtccgcaca aaaaggaggc gcaggcctta tcgtttataa
     6301 tcgcaaagaa ggaagagctc ttggggaggt cactaaattt ctggtttata acgccagaaa
     6361 aagacagaaa ggtggtgaca ctgcagccaa gtactttgaa agaacggaat gcgttgccgg
     6421 tgttcaggat atgcgtttcc aggaattaat gtccgatatt cttcattggc tggggatcac
     6481 taaaattcat cgcttcgttt caatgtccaa tatgaaatac gatgccttgc tcaaatctgg
     6541 aatcaatgtt attgaacggg taaaaatacc ggatgagttg attccaccag atgcccgggt
     6601 agaaatagat gccaagcgtg cagcaggata ttactcaccc gaaggaatta ttgatatcaa
     6661 tgaattggct aaaccgaaag gacgtgccct tcatgaataa tcaagagcaa caaatacaaa
     6721 atgttttgaa tacgcttcgt gatcctcgca ccatacgaac tcaatctcat cgtattttaa
     6781 acttggcaaa acagaatcgg ttggaacact tttccctgag ccctgaacga atgacaacaa
     6841 cggcatcgta tatcgtagac attatcacca gccgttatcc tgagcttgat attccatatc
     6901 atagccgctg gcgtcatttt gaaatggatg gccttccccg gattcataat ttacgcaaac
     6961 aactggagcc aatttccccc tcagaatggg gaaaaatact ctacgaactg gtcatcatca
     7021 gtgttttcct cgatgcaggg gcaggccccc tatggcgcta taaagaagct ctcactaaaa
     7081 aggagtacag tcgctctgaa ggattagccc tggccagttt atacctctat gaaagtgggg
     7141 catttagcgc tgacccaact aacccgtttc gcgtggatgc cgagcgtctg cttgacttta
     7201 aagaagcgga gctcataaaa ggttttcagg ttagctctac caatctgctt gaaggaatac
     7261 ccggtcgagt atccttgctt aatcgccttg gagaaatcat tcatcgcaag gaacactgtt
     7321 ttaatcgccg aagacgtctt ggtgagtttt atacttatgt ggaatccttg caagacaaca
     7381 aaatactgcc tactaccaaa ttattcgagg ccgtgttaga agcattcaat gacatatggc
     7441 ctattcgatt gatctatcat ggagtatctt taggcgatgt ctggatccac agcgcattga
     7501 aaaccaatga ggccggatct gaatacatac cctttcacaa attatcacaa tggctcactt
     7561 attctttagt agaaccactg gaacaaacag gtattgatgt tactgatttg gatgtattga
     7621 ccggattacc tgagtatcga aatggtggtt tactaatcga caccgaacta ttaaaagtaa
     7681 gaaataataa aattctggag cagcctcaag accctggctc agagcccata atagaatggc
     7741 gtgctttaac cgtagccttg ctggatgagt tagcgctatt aatcaggcaa cagctcaaca
     7801 tgaatgcaga atcgcttcct ttagccaaaa tactgcaagg aggcacttgg gaggcgggac
     7861 gacagattgc acaaaagaaa cgaattaacg gtacaccccc aatagaaatt attggtgatg
     7921 gcacgatatt ttaataagga ataataatgg acttcaatca agttaaggtt ataaatcatc
     7981 ctctcattca gcacaaactc accataatga ggaaaaaaga aaccagcaca gtaaaatttc
     8041 ggacactcat gcacgaagtc agtatgctat tagcctatga agtcactcgg gatttggaaa
     8101 tagaatacga agaaatagaa actcctttgg ccaccatgca atcacctgtt ttgaaaggaa
     8161 aaaaacttgt ttttgtctcg atattacgcg cgggtaatgg tctactggac ggcatgttac
     8221 aattagttcc tactgcccga attggtcata taggtctcta tcgagatccc aaaacgctgg
     8281 aagcagtcga atactacttc aagctacctg aacacacgca agacagagac gttattgttg
     8341 ttgaccccat gctggcaaca ggaaattcag ccattgccgc ggttaaagag gtcaaggcat
     8401 tacatcccaa gtcaattaaa tttctttgtc tactggccgc gcctgaagga atttccaatt
     8461 ttcatggaga acatcctgat gtcccaatat tcaccgcagc catagatgag cagctgaatg
     8521 atcatggata cattgttcct ggtttaggag acgcaggaga caggctatat ggcacaaaac
     8581 tggctcatta atgctgaatt atccttattt cagggatacc caatcagcca atagtacgtt
     8641 taaataataa ttaagattgt cttgcccttt cagtaatgac tgcttttact ttttgacaag
     8701 ggcatgctga aaaattacca ccaatttttc tgtggactcc ctctgagtgt ttatgcggga
     8761 caacagatgt gatgtatcaa gattctgaag aattttggca gggaaatgcc aggaaacaag
     8821 caacagcaaa tatagtctct tggcataccc cttattgcat aaagtcatat ctaatcactc
     8881 tacgagggag cgaagaatgt tagaactgaa cgacaaagaa actgcagtca ttaaggctat
     8941 tcatcataac cataagaaac gacttgaaag ccaaatgata acccgttttg gagtatattt
     9001 tgagcaatcg aaccatcctg aaaaattata ttcctggata gaaaaaattg aagacacgcg
     9061 cctgttagct aatcactatc tcgacactat cgctctaagc cgagctccaa gcacaatatc
     9121 ttcattggaa aaggaaacag cgggttcttc ttactacatt gaaaaagcaa aactgcttat
     9181 tccggaatct ataagaaatt ttctttctga tatcaattct tccaatgaca ataccttatt
     9241 caattttgta gccgccgagg tcttttctct tgctttaggc aaatcaatta ccgacctgtt
     9301 atttgattac ttcaataatg aatttttagg agcacctgag aatgtacaaa aagaatttaa
     9361 tgaaaccctg gaaaaacaaa ctgaattatc caatttgata aaaaaagaat gtgagcgcat
     9421 ctccatagaa attatcaaat tatttcattt cagagaaatg ttgctgcttg ggaaaataag
     9481 caaatcacca atcgaaccca attccagaga ggagttcatt accaaagcaa agattgctaa
     9541 ccaatctggc gaatctgttc atttggctat cgaaatctat tttgcacgtg aattaagtcg
     9601 tatttttaac tcaggtttta aatcacttta tcaagtccac gatgtggaaa ttcaaagtga
     9661 acatcaatcg cctctggtca agtggatcaa tcaaatgtca gagtctgagc aggttagatt
     9721 agcctttact caggaaatcc agctgaaatt catgcttctg gcaagagatt ttttactgga
     9781 aaaaatgaat cagaaaggga tactcgcaag ataccctatc attttttcac tctttgcagg
     9841 agtcattatt gctccaatag tcggtgtgac tatgattatt atgactggcg atacttttct
     9901 atgggcgata gctgctctgg ctgtcctggt ttttgctctc tcttgcatag caacgcacct
     9961 cctgatcaat aaactcagcg tattaactta ccaacgttct tcggataata gagctcatat
    10021 tcagcaatct atcgacatga taaatggaga atttttgcgt ttgaaaaaag aaagtttatt
    10081 aaaaaaagaa acaacctgtg aggttattga aaatacaaga gattttcaaa aaatcaacca
    10141 gaactttcat ttagtcacaa ttggtaaaaa ggtagccaga ggctcagtat ctggatggtt
    10201 aagagaatac gcttctcggt ataggcacag taaagccatc gaaattgatt tgggggatga
    10261 atacaaagaa ttaattactc aaagcaggat acaaactcaa gaactcattc attcgattaa
    10321 caagaaaaac tccaagttat tgattaaatg gattcacgat accatgactt atttgcagga
    10381 tgaaaaaaat caatctgtga ttgaagaatt tgaattaatc cccaaaatga aagagcaagt
    10441 ccttgagatt gtctgtcaac tcaattacat ccctcctatg ttattgaaat tctattctaa
    10501 ctccagagag aaaggtggcc tgggaggtaa tgaatctgat ttttctcata taaagcgctt
    10561 gacccccatc gatgcgccag ataaacccaa gcataacccc tataattatc tctgcgatac
    10621 agccctgaat ctgtttaaaa aatatgagcc tttatatact tcgacttgta tttttttagg
    10681 agacccggag tatcgacaaa tgttaggtat cgccagaaga gatgccgggg agcctctaac
    10741 agcggaaaac atcaatcgtt atcttgataa ctcctatgct tttttattat cgctttgcca
    10801 taaaatcaaa cctggattag gcttggatcc cttacaacaa tcagcgcgtg tcactgatga
    10861 attcgttctc tacagaatgc tgttgttaaa acagctggca gcaatttccg aatctgatag
    10921 caaagaaatc
//
LOCUS       DAXLDT010000102        10762 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXLDT010000102 DAXLDT010000000
VERSION     DAXLDT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.102"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            298..1143
                     /locus_tag="ACF2SP_002139"
     CDS             298..1143
                     /locus_tag="ACF2SP_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HFD2359915.1"
                     /translation="MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQ
                     ELTLSPYFCTRSDVDPTPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVA
                     FNPQGELIAVTRKQHIPSGEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPEL
                     SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCED
                     GLSFYGSSFISTPMGEILVQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHE
                     LVKSR"
     gene            1163..3055
                     /gene="speA"
                     /locus_tag="ACF2SP_002140"
     CDS             1163..3055
                     /gene="speA"
                     /locus_tag="ACF2SP_002140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFD2359916.1"
                     /translation="MNNNTLSDDNLYNIENWGEGYFRINSKGNIEISKKPGKPGVELQ
                     TIVDAADRAGLHLPLLIRCSDILHDRVHRIYQAFTQAREDTYYAGNYQLVYPIKVNQE
                     QSVVRELLKSPDNQIGLEAGSKPELMAVIGMLGDHQSTIVCNGYKDTSYIRTALIAQQ
                     MGHKVFIVIEKKSELDIILKESARLQIKPTLGVRIRLVSKSAGKWENTGGVKSKFGLN
                     AKQVLELILQLKANDMLDCLQLMHCHLGSQIANIHDIRHCMQEVARYYIELRQLNAPI
                     STIDVGGGLSVDYEGTRSNKGCSMNYSLVEYATHIMLALKHLCQEAEIPEPDLISESG
                     RALTAHHAVLVSNITDVEIINKAPVLPEISEDDSHVIRDIYDTYQAITDSSPTEIYNY
                     AAHSLNEAHSLFKHGVISLQEKAKVEAFYTNICMEIKDKLDEENPSEKALLMRINECM
                     AAKIFCNISFFQSIPDAWAIGQIFPVAPISQLNRTLSMHSILQDLTCDSDGTIKQYPG
                     SSCVTTTLMLPSYDINNPYNIGFFLVGAYQEILGNLHNLFGDTNSLDVKLSDDGQFEL
                     DDLVSGDTVTNVLNLAHFDTKKLIQSYEKQLIKSELPKEKALSYLNELRSIFSQLTYL
                     DGNIAQ"
     gene            3058..4098
                     /locus_tag="ACF2SP_002141"
     CDS             3058..4098
                     /locus_tag="ACF2SP_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine/peptidylarginine deiminase"
                     /protein_id="HFD2359917.1"
                     /translation="MNTAPKQYGFYMPAEWYPHERCWMAWPCHHETWSKIGLDKAKMA
                     YARVAKAIAQFEPVTLLVNPGDEDSATNLCKGHNIEIISLPINDSWTRDTGATFLINN
                     EKQLAGVDWIHNAWGGNYADCSLDNLIASHLIKCTEAQYFHAPLVMEGGSFHVDGEGT
                     ILTSKECLLNSNRNPHLSQQEIEQYLINYLGAERIIWLNMGLIGDETDGHIDEIATFI
                     APGKVLCLITKDKEDPNYHRLQENFEILKSSKDARGRTFEVYTVEQPPATYLNGERLT
                     LSYINFYMANQGIVMPAFGYESFDRLAYQLFVQIFPGYQITQIDALDVFSGGGGIHCI
                     TQQQPKSYKLGE"
     gene            complement(4468..6885)
                     /gene="gyrB"
                     /locus_tag="ACF2SP_002142"
     CDS             complement(4468..6885)
                     /gene="gyrB"
                     /locus_tag="ACF2SP_002142"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFD2359918.1"
                     /translation="MSVDASYDSNNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDESLAGYCKEIFVTIHSDESITVKDDGRGIPVDIHKEEGKSAAEVIMTVLHA
                     GGKFDDNSYKVSGGLHGVGVSVVNALSEELHLLVRRHGKVYEQHYRHGVPESPIAETG
                     EANSTGTQIWFKPSAETFSNIEFHYDILAKRLRELSYLNSGVCIHLFDERTQKKDTFH
                     YEGGIKAFVEHLNKNKTPVHPVVFSMTSEKDGIVIELAMQWNDGYQETIFCFTNNIPQ
                     RDGGTHMAGFRAALTRTLNNYIENEGYAKKAKVSPTGDDAREGLTAVLSVKVPDPKFS
                     SQTKDKLVSSEVKPAVESCVAEKFNDFLLENPTASKSIVAKIIDAARAREAARRAREM
                     TRRKGALDIAGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQARDRKFQAILPLKGK
                     ILNVEKARFDKMLSSQEVATLITALGCGIGPEEYDPDKVRYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQTRELLERGYIYIAQPPLYKVKRGKQEQYVKDDEALFDYLTQCALDGAT
                     FYPAKEAPPISAKSLEELVQHFRRVEKIIKRYKRRYPSDILKRVAYMPMLHQEDFNNL
                     PKIENWCRQLHLSLQQVDSKTEQYKVDIQKLTEGNQYTPKITVYQHGLENDIPMQVEF
                     FLSKDYKEMVDLGHKLSNLIEEGAYIQRGDKSLPVENFEQVLNWLMDEAKKGQTIQRY
                     KGLGEMNPEQLWETTMDPEVRRMLQVSIEDAVAADEIFTTLMGDNVEPRRDFIETNAL
                     DAENIDI"
     gene            complement(7182..8243)
                     /gene="recF"
                     /locus_tag="ACF2SP_002143"
     CDS             complement(7182..8243)
                     /gene="recF"
                     /locus_tag="ACF2SP_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFD2359919.1"
                     /translation="MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALY
                     MLSCAHSFRSREVAPIISYGQNQLNVFAHAYDESTISVQKSITDGTQIKLNNQFCCTT
                     SQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
                     LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFFQWQPKFYQVLSDLTNISCTVEYYK
                     GWDRKNAGQNIEELLQKSFDSDRNKLYTQYGPHQADLIISIEQYRVKHTLSRGQQKII
                     LIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKGQFVITNLINNNNYD
                     ILPIDSGLFEVNCGAIKIL"
     gene            complement(8240..9343)
                     /gene="dnaN"
                     /locus_tag="ACF2SP_002144"
     CDS             complement(8240..9343)
                     /gene="dnaN"
                     /locus_tag="ACF2SP_002144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFD2359920.1"
                     /translation="MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLY
                     ITATDLEIEMSAQVACDSNNTSGSITVPAKKFIDIIRSLDENASPSIVFDKALVTIKQ
                     NRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
                     LEFDSNIISAVATDGHRMAISRYSCNNIAQAKLLIPKKGVQELLRLLNCINDEQVLLA
                     AGKSHIKLITNQFVFLSKLIEARFPPYTKAIPREQDKYIVIDCTTLKRALSRIVILAH
                     EKSKAVLLHLQLGQLTLIANNNEQEEAIETLSAETQGDELKIGLNATYLLDVLSHFSE
                     GQIRLSMSNTESSILIESLVNDNYQYIIMPMKI"
     gene            complement(9357..10715)
                     /gene="dnaA"
                     /locus_tag="ACF2SP_002145"
     CDS             complement(9357..10715)
                     /gene="dnaA"
                     /locus_tag="ACF2SP_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFD2359921.1"
                     /translation="MSTTAWQKCLGLLQDEFSAQQFNTWLRPLQAYMDEQRLILLAPN
                     RFVVDWVRKHFFSRIEELIKQFSGDDIKAISIEVGSKPVEAVDTPAETIVTSSSTAPL
                     KSAPKKAVDYKSSHLNKKFVFDSFVEGNSNQLARAASMQVAERPGDAYNPLFIYGGVG
                     LGKTHLMHAIGNSILKNNPEAKVLYLHSERFVADMVKALQTNSINEFKRFYRSLNALL
                     IDDIQFFAGKDRSQEEFFHTFNALLEGQQQIILTSDRYPKEIEGMEERLKSRFGWGLT
                     VAVEPPELETRVAILISKAEQSNIELPYEVAFFIAKRIRSNVRELEGALRRVIANAHF
                     TGKPITIEFVHEALRDLLALQDKLVTIENIQKTVAEYYKVKVADLLSKRRSRSIARPR
                     QMAMALSKELTNHSLPEIGDHFGGKDHTTVIHACRKVKELIQDDSDFAEDYKNLMRIL
                     SS"
BASE COUNT         3302 a         2105 c         1968 g         3387 t
ORIGIN      
        1 tacctcacaa aaaatcaagg aaaaacgaag tgtgctgtcc taaatgaaag gaaagattcg
       61 cttttaatca atagtcgcga gccttcacca ctttcagaat agctgccatt ctgaaaattc
      121 attagtaatt agtaacagcc aaaatgagca atgaattgaa attaatgcta gtacaaagag
      181 agcttttttt gataacatag ccagattttt aatctgggtt gatgatttaa cttaaacaag
      241 agtaagccat tcgaattcga gataaaaatt acttaacatc atttattggg aactctaatg
      301 aaagaaaaca aactaacggt cgcactggtt caggaacaat ggtatgaaaa tcctaaagag
      361 catcaagata aactggcttc tggaatattt tcagctaccc agcaaggagc aaaactcgtt
      421 tgtttacaag agcttacctt aagcccttat ttctgcacac gtagtgacgt cgatcccacc
      481 ccttatatgg aggatatcgc cactggtccc actgctcaat ttgttagcca aatggcaaag
      541 tcgaatcaaa ttcatattac cgcatcgctt tttgaaaaag ctggttacaa taccgcggtc
      601 gcttttaatc cccaaggaga attgattgct gttaccagaa agcaacatat ccccagtggt
      661 gaaaaatatc atgaaaactt ttacttcaag ccgggcgatt ccaattaccc tgtccatacc
      721 attgctggtc actacttggg attaccaaca tgctatgacc aatggttccc ggaattatca
      781 agaatttatg gattaaaagg agctgaaatt ttggtctatc ccacagcgat tggaggggaa
      841 ccgactgcgc caggatttga tagccaaccc atgtggcaaa aagttatggt agcacaagga
      901 attatgagca acaccttcat tattgcggtc aaccggattg ggtgtgaaga tggattaagt
      961 ttttacggaa gcagctttat cagtacccct atgggcgaaa ttttagttca ggcaccgcgt
     1021 aatgaacctg ccgtattggt tgctgaactg gattttagcc aacgtgaatt gtggggacga
     1081 ttattcccat ttccccaaca acgagagcct gaaacttatc atgaattagt caaatcccgt
     1141 taatcctctt tggaacgtta gaatgaacaa taatactcta tcagatgaca atctttacaa
     1201 catagaaaat tggggtgagg gatacttccg aattaactcc aagggaaata ttgaaatcag
     1261 taaaaaaccc ggcaagccgg gtgttgaact gcaaactata gttgatgcag ctgaccgtgc
     1321 tggcttgcat ttgcctttat tgatccgttg tagtgatatt cttcatgata gagtccatag
     1381 aatataccag gcgttcaccc aggctcgaga agacacttat tatgccggta attatcagct
     1441 ggtttatcca attaaagtaa accaggaaca aagtgtggta cgggaattgt taaaatcgcc
     1501 tgataatcag ataggtctgg aggctggcag caaacctgaa ttgatggcgg ttataggtat
     1561 gctgggtgac catcagagta ctatagtttg caacggctat aaagacacaa gttatattcg
     1621 tacagcgctt attgctcaac aaatgggtca taaggttttt attgttattg aaaaaaaatc
     1681 tgagttggac attattttaa aggaatctgc acgccttcaa atcaaaccga cattaggtgt
     1741 gcgcattcgg ctggtcagca agagtgctgg caagtgggaa aataccggcg gggttaaatc
     1801 aaaatttgga ctaaatgcca aacaggtact tgaactgatt ttacaattaa aagccaatga
     1861 tatgctggat tgtttgcagc taatgcactg ccatttaggt tcacaaattg ccaacatcca
     1921 tgacatacgc cattgcatgc aagaagtggc gagatactat attgagctac gtcaactcaa
     1981 tgctccgata agtaccatcg atgtgggtgg aggtttgagt gtcgattatg aggggactcg
     2041 atccaataaa ggatgctcaa tgaactacag tcttgttgaa tatgctactc acatcatgtt
     2101 ggctttaaaa cacttatgtc aagaagctga gataccagag cctgatctta tctccgaatc
     2161 aggacgagca ttaacagctc atcatgctgt attagtttct aatattacgg atgtagagat
     2221 catcaataaa gcgcctgttc ttcctgaaat atcggaagat gattcgcatg ttattcgaga
     2281 tatttatgat acttatcaag ccataacaga cagctcgccc actgaaatat ataattacgc
     2341 agctcattcg cttaatgaag cgcattctct tttcaagcat ggggtaatta gcctacagga
     2401 aaaggccaag gtagaggctt tttatactaa tatttgtatg gaaataaaag ataaactgga
     2461 tgaagaaaat ccaagtgaaa aagctttgct aatgagaatc aatgaatgca tggctgccaa
     2521 gatattttgt aatatctctt ttttccaatc tatacctgat gcttgggcta ttggtcagat
     2581 tttcccggta gcccctatct ctcagcttaa tagaacacta agtatgcata gtatcctaca
     2641 ggatttaacc tgcgattcag atggaacaat taagcaatac cctggaagtt cctgcgttac
     2701 aaccacattg atgctccctt catacgatat caacaaccct tataacatcg gatttttcct
     2761 ggttggtgcc tatcaggaaa tattgggtaa tctgcataac ttgtttggag ataccaattc
     2821 tctagatgta aaactttctg atgatggaca atttgagctg gatgatttag tcagcggcga
     2881 caccgtaaca aacgtgttga atttagccca tttcgatacc aaaaagttaa ttcaatctta
     2941 tgaaaagcaa ttaataaaat ctgaacttcc taaggagaag gcactttctt acttaaatga
     3001 attaagaagt attttttctc aattaactta ccttgatgga aatattgcac agtgaatatg
     3061 aatacagcac ccaaacaata tggtttttat atgcctgctg aatggtatcc tcatgaacga
     3121 tgctggatgg catggccctg tcaccatgaa acgtggtcca aaatagggct agacaaggct
     3181 aaaatggcct atgcacgtgt tgcaaaagct atagctcaat ttgagcccgt tactttatta
     3241 gtaaatccag gcgatgagga ctccgcaacg aatttatgca aaggtcataa tattgaaatt
     3301 atctctttac caattaacga ttcatggacc cgcgatacgg gcgctacctt tttaattaat
     3361 aatgaaaaac agcttgccgg agttgactgg atacataatg cttggggtgg aaattatgct
     3421 gactgttctt tggataattt aatagcttct catctaataa aatgcactga agctcaatac
     3481 ttccatgcgc cattagtcat ggaaggaggc tcctttcatg tagatggtga ggggactatt
     3541 ttgaccagta aagagtgctt attaaattcc aatcgaaatc cacatttatc tcaacaggaa
     3601 atcgaacaat accttattaa ttatctgggt gctgaacgta ttatttggct gaacatggga
     3661 ctgataggtg atgaaacaga tggtcatatt gacgaaatag caacatttat tgctcctgga
     3721 aaggtgttat gccttattac caaggataag gaagatccta actaccatcg cttgcaggaa
     3781 aattttgaaa ttctcaaatc atctaaggat gccagaggac gaacatttga ggtctatact
     3841 gttgagcaac cacctgcaac ttaccttaat ggagaaaggc ttacactctc gtacatcaat
     3901 ttttacatgg ctaatcaagg tattgtgatg cccgcttttg gatatgaatc ttttgacaga
     3961 ttagcttatc agttattcgt acaaattttt ccagggtatc aaattacaca aattgatgcg
     4021 ctcgatgtat tttctggtgg tgggggtatc cactgtatta cacaacaaca gcctaaatct
     4081 tataaattag gagagtaatg acgaagacaa tgcctcttct taattgagaa atttcttaca
     4141 tattactgct cattatactc gatgacttat actgccaatt ggttatacta aaaacataca
     4201 gggatgacat ggatatgtct gacaggaagt caactaaggg accagtacaa aggatgtact
     4261 tcgctgcaag gatgcagcac ttattctcta atttacatta gtataccttt ctcaatttat
     4321 tcatttaagc aatcaaaagg cattatttga acacatcatc atagtaggga agtaagttaa
     4381 gtcattgggc gaatcatcta taagctagaa aagagtttgc cactagcggg gcaaacgtca
     4441 ctccagttta ttaatactat ttgttgttta tatatcaatg ttttcagcat ctaaggcatt
     4501 cgtttcaatg aaatctcttc ttggttctac attgtcgccc attaaggtgg taaagatttc
     4561 atctgcagct acagcatctt cgatactcac ttgcaacatt cgtctaacct caggatccat
     4621 ggtcgtttcc cataactgct cgggattcat ttccccaaga cctttatagc gctgaattgt
     4681 ttgtcctttt tttgcttcat ccattagcca attaagtact tgctcaaagt tttccacagg
     4741 caaggattta tctccacgtt gaatataggc gccttcctct atcaaattcg ataatttgtg
     4801 tcctaaatca accatttctt tgtaatcttt agacaagaaa aattcaactt gcattggaat
     4861 atcattttcc aagccatgct ggtacacagt aatttttggg gtgtattgat taccttctgt
     4921 aagtttttgg atatctactt tgtattgttc tgttttactg tctacttgtt gtaagcttag
     4981 atgtaattgt ctgcaccagt tttcaatttt tggcagattg ttaaaatcct cttggtgcag
     5041 cattggcata taagctactc tttttagtat atcgctgggg tatcttctct tgtaccgctt
     5101 tataatcttc tcaactcgtc tgaaatgttg aaccaattct tccagcgatt tggcagatat
     5161 gggcggagcc tctttggcgg gataaaatgt cgccccatcc aacgcgcact gcgttagata
     5221 atcaaataac gcttcatcat ctttaacata ttgctcctgt tttccacgct ttactttata
     5281 caaaggtggt tgtgcaatat aaatatatcc tcgttccaat agctctctgg tttgtctata
     5341 gaaaaaggta agaagtaaag ttcttatgtg agacccatcc acatcagcat ctgtcataat
     5401 gatgattcta tgataacgta ctttatctgg atcatattcc tcgggaccaa ttccacagcc
     5461 taatgcagta atcaaagtgg ctacttcttg agaagaaagc atcttgtcaa accgggcttt
     5521 ttcaacatta agaatcttgc ctttaagagg taaaatagcc tggaattttc tgtctcgcgc
     5581 ttgttttgct gagcctcctg cagagtcacc ctcaactaaa tacaactcag aaagcgatgg
     5641 gtctttttct tggcaatcag ctaatttacc tggtaaacca gcaatatcca aagccccttt
     5701 acgtcgagtc atttcacgag cacgccgcgc tgcctctcta gctcttgctg catcaataat
     5761 ttttgctacg atggattttg atgcagtggg attttctaaa agaaaatcgt taaatttttc
     5821 agcaacgcag gattctactg ctggttttac ttcagatgaa accagtttat ctttcgtttg
     5881 cgatgaaaac ttgggatcag ggacttttac tgaaagaaca gctgttaatc cttcgcgagc
     5941 atcatcacct gttggagata cttttgcctt tttagcatac ccttcgtttt ctatgtaatt
     6001 atttaaagtg cgagttaatg ctgctctaaa gccagccata tgtgttccac catccctttg
     6061 tgggatgttg ttagtaaagc aaaatatagt ttcttgatag ccatcattcc attgcattgc
     6121 cagttcaatg acgataccgt ctttttccga tgtcattgag aaaacaacgg ggtggactgg
     6181 tgttttattt ttatttaagt gctcaacaaa ggctttgata ccgccttcgt agtgaaacgt
     6241 gtcttttttc tgtgttcttt catcaaataa gtgaatacaa acacctgagt tcaggtatga
     6301 caattcacgt aatcttttag ctagaatgtc gtaatggaac tcaatattag aaaaggtttc
     6361 agcgcttggt ttaaaccata tttgtgtgcc agtagagttg gcttctccag tttcagcaat
     6421 aggagattca ggaacaccat gcctgtaatg ttgctcatac acttttccat gtctacgtac
     6481 tagcaaatgc aactcttcag aaagggcatt caccacagat acccctactc catgtagacc
     6541 gcctgatacc ttgtaagagt tatcatcaaa tttacctccg gcatgcagga ctgtcataat
     6601 gacttcagca gctgattttc cttcttcctt atgaatatcg acaggaatac cacgaccatc
     6661 atccttaacg gtaattgatt catcgctatg aatggtaacg aaaatttcct tgcaataacc
     6721 tgccagggat tcatctattg aattatctac aacctcaaaa accatatgat gcaaaccggt
     6781 tccatcatct gtatccccaa tgtacatgcc cggtcttttt cttactgcgt ctaaaccttt
     6841 taggacttta atattatttg aatcataact ggcgtctacg ctcatcagct gccctcttgc
     6901 cgtattatgg tattaacaat gagtctatta tatcatcttt tgtatctggt ttatatctta
     6961 agtagagaac tttacgcttg tgtattctga attattgacg ttgatttttt tgaatatcat
     7021 tttgtttatt tgattttcat atcatggcat ttgatgagcc atttgtagaa tggcatatgt
     7081 ttacctttag aattttatat tgcatttgca ttaaggtgat attgtttaat aacgagaact
     7141 tttcattcca ttaatttgtg ttccacgtga aacattctat attataaaat tttaattgcg
     7201 ccacagttta cttcaaacaa acctgaatca attggcagga tatcgtaatt attgttgtta
     7261 attaaattcg ttataacaaa ctgccccttt tgctgtgtca gatacttaat taaatttcgc
     7321 tggtgatagt catctaactc agcagccaaa tcatcaataa gatacaagca atccttatct
     7381 aatagctgac cttgagccaa tttaagggcg attagaatta ttttttgttg tccacgagag
     7441 agtgtatgct ttactctgta ttgttcaatg ctaattatca aatcagcttg atggggtcca
     7501 tattgcgtat atagtttatt tctatcacta tcaaatgatt tctgaagcag ttcctcaatg
     7561 ttctgccctg cattttttct atcccaccct ttgtaatact caactgtgca ggatatattg
     7621 gttagatcac ttaagacttg gtagaattta ggttgccatt gaaaaaaata gtcatttctg
     7681 gctttatcca gttgatttgc caattgactt agctgttggt cccaaggaat aaaatgctca
     7741 taagtagctc tggattttaa taaggcattt cgttggctaa gtatgcgctt gtaatccttc
     7801 caaattttca aataatcatg tttcacgtga aacaaacccc aatcaagcaa gcttctcctt
     7861 actgagggac cggcatcaat aatttgaaaa atatcagagt aaataacctg gcaaggaagc
     7921 gcataggcta gctgacttgt agtacaacaa aactgattat ttaatttaat ttgtgtgccg
     7981 tctgttatag atttctgaac actaatagtg ctttcgtcgt aagcgtgggc aaaaacatta
     8041 agttggtttt gaccataaga aataattgga gctacttcac gagaccgaaa agaatgggcg
     8101 caactcaaca tgtaaagagc ctctaataac gaggttttac cactgccatt aggtcctgta
     8161 atacaattaa aattgggatt taggattaaa ctagtcgaag cgatattccg aaaattatga
     8221 attctaactt cagataaaat catattttca taggcataat aatgtattga taattatcgt
     8281 tgactaatga ctcaattaaa atactgcttt ctgtattaga cattgataac ctgatttgac
     8341 cttcagaaaa gtgacttaaa acatccagca aataggttgc attaagtccg attttgagtt
     8401 catcaccttg tgtttcggca ctcaatgttt caattgcctc ttcttgctca ttattattgg
     8461 caattaaagt taactgacct agttgtaaat gcaacaaaac agcctttgac ttttcatgag
     8521 ctaaaatgac aatacgagat aatgcacgct tgagcgtagt gcaatcaata acaatatatt
     8581 tatcctgctc tcttggtata gctttagtat aaggtggaaa gcgtgcttcg attaatttag
     8641 acaaaaatac aaattgatta gtaatcaact taatatgaga ctttcccgca gccaacaaaa
     8701 cttgttcgtc attaatacaa ttcaatagtc ttaataattc ttgcacccct tttttgggta
     8761 taagtaattt tgcttgagca atattattac aggaatatcg cgatatagcc attctgtgcc
     8821 catcagtagc aaccgctgaa ataatattac tgtcaaactc cagtaataat ccattcaaaa
     8881 atatgcgaac atcctgctga gacatagcaa aatgagtaga ttgcaataac tgcaacaaaa
     8941 ccaaacgcgg gatagtcaac tcgacatcat tgcattcatc ttcgctactg ggataattct
     9001 cagcaggcaa agttgccagt ttaaatgtac ttcggttttg ttttatcgtc actaaagctt
     9061 tatcaaaaac gatagatggc gaagcgtttt catccaatga ccggataata tcaatgaatt
     9121 tcttggctgg tactgtaata gaaccactag tattattgga atcacatgcc acttgtgcgg
     9181 acatttcaat ttccaaatct gtagctgtaa tatataaaag tccatccgac agttttaata
     9241 aaaaatggga taaaatagcc aaagactgct tcttatctac ggctccggaa acagtaagca
     9301 atggagtaag tagatcttct tttttaatgg ttagctcaaa caagatagac tccgtatcaa
     9361 gaagataata ttcgcattaa atttttataa tcttcagcaa aatcgctatc atcctggatt
     9421 aattctttaa ccttcctaca agcatgtatg acagtcgtat gatccttacc accgaaatga
     9481 tcaccgattt caggtaaact atgattggtc aattctttac tcaaagccat tgccatttgc
     9541 cttggtcttg caatagaccg gcttctgcgt tttgaaagta agtcagcgac ctttacttta
     9601 taatattcag ctaccgtttt ttggatattt tctatagtaa ctaacttatc ttgcaaagca
     9661 agaagatcac gcaatgcctc atgtacaaat tcaatagtta tcggcttacc agtaaagtga
     9721 gcattggcta taactcgacg caaggcacct tcgagttctc tgacattcga acgtatacgt
     9781 ttggctataa aaaaggctac ttcataaggc aattcaatat tagattgctc agctttacta
     9841 attaatattg ccacgcgcgt ttctaactct ggaggctcca ctgctacagt caatccccaa
     9901 ccaaatcgag attttaaacg ctcttccatc ccttctattt ctttaggata tcgatcactg
     9961 gtcaaaatga tttgttgttg cccttccagt aaggcattaa aagtatgaaa aaattcctct
    10021 tgtgacctgt ccttccccgc aaaaaactga atatcatcaa taagtaaagc atttaaagaa
    10081 cgataaaaac gtttaaattc attaatagaa tttgtttgca atgctttaac catgtctgca
    10141 acaaaacgtt ctgaatgcaa atacaatact ttagcctcag gattattctt taaaatacta
    10201 ttaccaatgg catgcatgag atgtgtttta cctaagccta ctcccccata aataaataag
    10261 ggattataag catcccctgg gcgctcagct acctgcatgg acgcagctct cgccagttga
    10321 tttgaatttc cttctacaaa gctatcaaaa acaaattttt tatttaagtg actgcttttg
    10381 tagtcaactg cttttttagg agcggatttt agaggtgctg tactagatga agtaacaatt
    10441 gtttcagcag gtgtatcaac tgcttcgaca ggcttactac ctacttcaat agaaatagct
    10501 ttgatgtcat cgccggagaa ctgtttgatc aactcttcaa tacgagaaaa aaaatgtttt
    10561 ctaacccaat caacaacgaa acgattaggc gctaataaga ttaaacgctg ctcgtccatg
    10621 taagcttgta aagggcgcaa ccaagtatta aattgctgag cagaaaattc atcttgcaac
    10681 aaacctaaac atttttgcca agcagtagtt gacacaaaat aactcctaat ttaaacaagg
    10741 gtggtaaaag aatacttaaa ac
//
LOCUS       DAXLDT010000103        10760 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXLDT010000103 DAXLDT010000000
VERSION     DAXLDT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.103"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            22..5787
                     /locus_tag="ACF2SP_002146"
     CDS             22..5787
                     /locus_tag="ACF2SP_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFD2359922.1"
                     /translation="MNKNPFSLIMNVLCSIFSTLFGKIKWNHPTWLVYLKHKSKNSPQ
                     IFWGTTLLFIVLLGAGAYGAYWYNNLPKPTYTTASITIPDITPNEENLVPNNLIIDFG
                     IRSNEFIPQSVAPINLIGKEIDEGIEMAPQIAGKWSWNSDSQLIFIPAEDWPAGQKYT
                     IHFAKNFFAPNANIESYDLSFTTKPFQATIREFKFYQDPVNAETRHAVATIEFNYPVN
                     QKNLEKNTSVVFQTIKNGKADLTAESVPFSYTYDNNKRIAYLHSANIKIEDVSRYLLL
                     TLNKNVSSSTDSAILGQSISKNLLIPDASNFLKIVSTSANIVRNDKDRPEQVLSIETS
                     LGVNESEFNKSVHVYLLPQNYPATAAEPEKSNYEWQNPGEVTNNILSLSTPLPKELIP
                     SEQNYTTLHGFKFKAQTPRYLYIKIDKGMKGFGGFKLSNDYTAIVPVPEYPKEISFLH
                     KGSLLALSGEKKLSVLIRGVPAVKYEFARVLPDNVNQLVTQTQGDFSNPYFINPSFNQ
                     QNISQIFSEIQQYDSTDLTRQQYTSLDLGKYLASEANASGPQGLFLLQATGWDVANQY
                     ALDVKASRLILITDLGMVVKDNNDGSHDVFISSITQGLPVAGATVTILGKNGLPILSR
                     VTDEQGRANFPTLKDFIEDREPTVYLANLNNDVSFIPFNNYNRQLNFSKFDIGGIYTN
                     DQDLQNLTAYLFSDRGIYRPGDTVHVGMIVKRAFAEPQPSGLPLQATVVDARGTTIKD
                     QKVTLNDTGYMELDFTTSANSPTGQYMVYLYLVKDNHPQNLLGSTSVRISEFQPDRMR
                     ITSSFEPKPFDGWSSPTDLKAKVDLWNLYGAPAANRRVSGKILLTPQRVQFDNYPDYI
                     FADPLYDPKKPPKIFTENLAEATTNENGQAEFSLNLERFEKATYQLTFFAEGFEPGSG
                     RSVTTQTKTLVSPLPYFIGYKADGDLHFIKQNSVRNVNYIAINPQLGKEDVKNLKIQL
                     ISLRPVTTLVKKADGTYQYQSTIQSTVINTNPFEIKESGVNYTLPTNTIGDYSLNVLD
                     KDDSILSQLQFSVVGTSQAPLAKNAELSIKLNKEEYQANEDIEIQITAPYTGSGLITI
                     ERDKVYAVHWFKTETQNSVQTIHIPADFQGNGYVNVAFIRDWESPELFISPLSYSVAP
                     FNVNHDKHDIRIGLKTPELARPGESFTIDYHTDKPGKIIVFAVDEGILQVANYTTPDP
                     LSFFFQKRALEVLTQQTVDQILPKFIQDREISAVGGDEGEAALASRLNPFKRKTDLPV
                     VYWSGILDTDSADRQLTYQIPDYFNGSIRVVAVAVANDSVGSREVSAEVRGDFIISPN
                     VPTFVAPGDEFEISATVANNIKDSGEHAQVEVELTASSELEAIGSAKESLEISEGNEQ
                     TVYFKLRAKSLLGSARIAITAKSGDKSSKMDSTLSVRPANPFTTYINSGKSQDSQKSL
                     EVIQTLYPEHRKVEATISTSPLVLIFGLQRYLDNFPYGCTEQLTSKALPWLVMNSLPW
                     FSSESGPIHEKINTTIQMLSQRQMSNGGFSYWPGLSDNSANSFASVYAMHFLTEARAQ
                     GFNVPREMFYNGINYLKELASRNPTDADSARIQAYAIYILTRNELVTTNYLANLQLYL
                     QQDSSKNWLKDITAVYIAATYQLLKSYSEANQLIGQYQPPTNNAFTTDFYDRNIADAQ
                     YLYLVAKHFPNLLPQVSDNILMNLVEAINNDEINTVLSGFSSLALGAYPQNESVDLSD
                     KLSITEVLANNQQRNIPSDQNNYQKLNIDETVKTIRFNNPDKQTFFYQLVQSGFDKII
                     PTEPLKQGIEIYREYRDEKGNVIDSAVLGEEIEVHLQVRSLDNNYLSNIAIEDLLPGG
                     FEVVRDSVKTDNMDYADVREDRVNFFGWVDLTAKELVYKIKATNVGKYIVPPAYAEAM
                     YNPNTKARGSSSVITVSREED"
     gene            complement(6035..7438)
                     /locus_tag="ACF2SP_002147"
     CDS             complement(6035..7438)
                     /locus_tag="ACF2SP_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1666 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359923.1"
                     /translation="MLKFVIYLSSRVQLMGKKAYIIGMGPAGLASALALLAKGYSVEL
                     IDRRSEDDVFSRKQGVFLKDDTIRDFFALSQLASKGYSLEFKDNFICKLIRPIDESNL
                     QLSAEDQLDLAFFELIEKERTVIPIAELQRYQHSKLKYIYDKDGFTFQDEEDPYTVKF
                     NNAEQQLIFHLGDTQIVKVDAENQELTLDHNGSIQNHSFDLLVDASGKTSKSFTQLWN
                     SQNPEFAIGYEKLDNPDHNAFGVMYLSINNPPLAMIANPVLSPIEGTSFIPLDKITPL
                     KAMGWTHDYPPLIYLKYSKKNGAVYLTGEIPESILKDNNKKELKQWFDLVLQLLFNNT
                     DFKTSAPGLASVFKTDIEIADKFALLLPKGGVFCLVGDALMSANFLLGCGISNALDDA
                     NQLPDMVDNQFSHREAEAYRNLRYLDYKDDWLSFREHIALRLKSLQLEKQLDLSMQEL
                     ELKQRELENIQQQCSFY"
     gene            complement(7599..8990)
                     /locus_tag="ACF2SP_002148"
     CDS             complement(7599..8990)
                     /locus_tag="ACF2SP_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1667 family Dot/Icm T4SS effector"
                     /protein_id="HFD2359924.1"
                     /translation="MEYKEVIPMIRFFIICFIFLVSHLAYANPYWDRYKVPRDSHYPN
                     LKFHYINPDSLKIVQENAGIDVLRIQTDWFEETDSYQVGTVIIESSGTPALLSRSIHK
                     PKWGSYLGILKSRFGGNTVYYDAIGTGKEYRKLARAINLRFPVPSHDMIFELYAENPQ
                     TGFMEKVVEKNIIASELTRDNRHFNDLEVRQLLPAVKTPSLRVNIYAEGYTQSEKMKF
                     WNRAIKTVQALQSQRFPGIDYMDFYAVFNPSHKRLGVAEDLGLPVPEFDSFLGLYYPY
                     WDNFGRWYHIVYPTRENKFRQGLASAPYDYPIVLVNNSQYWGVGNYKSHTAIPADNST
                     YFTYILIHELGHFFGLNEEYEGGGRTELEFAPEMKEPWSQNITFLTTPSYSGLKWNEL
                     VNPQMSIPTPYSDWQSTPPQYGAYRGGYGDSPSHQGASHKPGLNCVMESHASFCDICQ
                     KGIMDVIRFDLGT"
     gene            complement(9138..9962)
                     /locus_tag="ACF2SP_002149"
     CDS             complement(9138..9962)
                     /locus_tag="ACF2SP_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HFD2359925.1"
                     /translation="MKKIAIILSLFPFLICCGLANATASQKRLPGESYQDAVKRIINN
                     KPQNELKMLIARGTPYSKKVSLQKIDYSTVPKVSSYDELTNMFNLIRDTRFLYSPDKP
                     DFQRRISWLYPDDGCFARAALSRIKLDSEHFVIPAKIFAFGDLEMQTPYSSEGTVSWW
                     YHVSAVVNYMGTIYVLDPAAKPEGPMLIDDWYNKIGIMDKMEAVVCNPYTYDPFDRCY
                     DATNKTDNKALNDQLKYLNKEWVRIESLGFDPSVLLGDNPPWIGDMGNSTLLSYKS"
     gene            10227..>10760
                     /locus_tag="ACF2SP_002150"
     CDS             10227..>10760
                     /locus_tag="ACF2SP_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359926.1"
                     /translation="MASDNPFSGLREKMLNLYHQAERDLFREPVFSNQFQDAKLDDVI
                     ISRAGVKFLWGKISEDQRAHLQGLFLAYLEDQASSIYKEYLDKKLGLGPNTATSSLLV
                     QFESKKIKSLENIRAVLDKCVTENKEEDYWFTNAARGILLTNAFAISESSSESYQSEL
                     EQRISKLIPPPGNANEEE"
BASE COUNT         3512 a         2134 c         1931 g         3183 t
ORIGIN      
        1 caaccaggac tccatattac catgaacaaa aacccattct ccctaattat gaatgtattg
       61 tgttcaatat tttcaactct attcggaaaa ataaaatgga atcacccaac ctggttagtt
      121 tatttaaaac acaaatcaaa aaattctccc caaatatttt ggggaacaac tctattattt
      181 atcgtgttat tgggcgcagg agcatatggt gcttattggt ataataattt gccaaaacca
      241 acttatacta ctgcatccat cacaatacca gacatcacac ccaatgagga aaaccttgtt
      301 cccaataatc tcattattga tttcggcatt agaagtaatg aatttatccc acaatcagtt
      361 gcccccatta atttaattgg caaagaaatt gatgaaggga tagaaatggc tccgcaaatt
      421 gcgggtaaat ggagctggaa tagtgatagc caattaattt ttattcccgc tgaagattgg
      481 cctgcagggc aaaaatatac tattcatttt gcaaaaaatt tctttgcacc caatgcgaat
      541 attgaaagtt atgatttatc ctttacaaca aagccctttc aggccacaat ccgcgagttc
      601 aaattttatc aagatcccgt caatgcagaa acaaggcacg ctgtagccac tatagagttc
      661 aactatccag taaatcaaaa aaatcttgaa aaaaatactt ccgttgtatt ccaaaccatt
      721 aaaaatggaa aagcagacct cactgcagaa tccgttcctt tttcttatac ttatgataat
      781 aataaacgaa ttgcctatct tcattcggca aatatcaaaa tagaagatgt atcgcgttat
      841 ttacttttaa ctttgaataa aaacgttagt tcttcaactg attcagcaat tcttgggcaa
      901 tcaattagta aaaatttgct gatacccgat gcaagtaact ttctaaaaat agtttcaaca
      961 tcggctaata tagtccgtaa tgataaagac aggcccgaac aggtacttag tattgagacc
     1021 agtttgggtg ttaatgaaag tgaatttaat aaatcagtac atgtctattt gctccctcag
     1081 aattatcctg ctactgctgc agaaccagaa aaatccaatt acgaatggca aaatccaggt
     1141 gaagtaacga ataatatttt gtctctgtcg actccattac ccaaagagtt aatcccttct
     1201 gagcaaaatt acactacgtt gcatggcttc aaatttaaag ctcaaactcc cagatacctg
     1261 tatatcaaga ttgataaagg aatgaagggt tttggtggtt ttaaattaag caatgactac
     1321 acggctattg ttccagtacc agaatacccc aaagaaatca gtttcttgca taagggttct
     1381 ttgttagcac tgagtggtga gaaaaaactg tcagttctga tacgcggagt gcctgcagtt
     1441 aagtatgaat tcgcgcgtgt gttgcctgat aatgtcaatc agctggttac gcaaacccaa
     1501 ggcgatttca gcaatcctta ttttattaat ccctctttta atcaacaaaa catcagtcaa
     1561 attttctcgg aaatccagca atatgactcc actgatttaa ccagacagca atacacatcc
     1621 ctggatctgg gaaaatattt agcatctgaa gccaatgcct caggaccgca aggattgttc
     1681 ttattacaag caacaggttg ggatgtagct aatcagtatg ctctcgatgt taaggccagt
     1741 cgtttgatat tgattacgga tttggggatg gtggtaaaag acaataatga cgggagtcat
     1801 gatgtcttta ttagctccat tacacaagga ctcccagttg caggggcaac agttacgatt
     1861 ttaggtaaaa atggcttgcc cattttatct cgtgttacag atgaacaagg gcgagcaaat
     1921 tttcctactc tgaaagattt tattgaagac agagaaccaa ccgtgtattt ggcaaacctg
     1981 aataatgatg tgtcttttat tccattcaat aactacaaca ggcaattgaa tttttcaaaa
     2041 tttgatattg gaggaatcta taccaatgat caggatttgc agaacttaac agcttattta
     2101 ttttctgata gaggaatata taggcccgga gacacagtgc atgttggaat gattgtaaaa
     2161 cgagcctttg cagaaccaca accttctggt ctgccattac aagccactgt agtagatgcc
     2221 aggggaacta ctatcaaaga ccaaaaagtg acattaaatg acaccggtta tatggagctt
     2281 gattttacta ccagtgccaa ttctcccact ggtcagtata tggtttattt atacttggta
     2341 aaagataacc acccgcaaaa tcttctcggt tcaaccagtg tccgtatttc cgaattccaa
     2401 cccgacagaa tgcgcattac ttccagcttt gaacccaaac cttttgatgg ctggagctca
     2461 cccactgatc tgaaagcaaa ggtagatctt tggaacttgt atggtgcccc agctgccaat
     2521 agaagggtaa gcggcaaaat tttattaact cctcagcgag tgcaatttga caactatcct
     2581 gattatattt ttgcggatcc actgtatgat ccgaaaaagc ccccaaaaat ttttacagaa
     2641 aacctggcag aagcaacaac taacgagaat gggcaggctg aatttagctt gaatcttgaa
     2701 cgttttgaaa aggcaactta tcaattaact ttttttgctg aaggctttga gcctggtagt
     2761 gggagaagtg ttactacaca aactaaaaca ttggtcagcc ctttacccta tttcattggc
     2821 tataaagctg atggcgactt gcattttatt aaacaaaaca gcgtcagaaa tgttaattac
     2881 attgcaatta atccacagtt gggtaaagaa gacgtaaaaa atttaaagat acaattaatt
     2941 tccctgcgcc ccgtcacaac tctggttaaa aaagctgacg gcacttatca atatcaatcc
     3001 acaatacaat caacagtcat caataccaat ccattcgaaa ttaaagaatc tggtgttaac
     3061 tacaccctgc caacgaatac tattggtgat tactctctca acgtattaga taaggatgat
     3121 agtattctta gccagctgca atttagcgta gttggtacca gtcaggctcc tctcgctaaa
     3181 aacgcggaat tatctatcaa acttaataaa gaggaatatc aagctaatga agatatagaa
     3241 atccaaatca ctgctcctta tactggctca ggcttgatca ctattgaaag agataaagtc
     3301 tatgctgtgc attggtttaa gacggaaacc caaaactctg tacaaaccat ccacatacct
     3361 gctgattttc agggtaatgg atatgtgaat gtggcgttta ttcgtgattg ggaatctcct
     3421 gaactcttca ttagcccgtt aagttatagc gttgctccat tcaatgtgaa ccatgataag
     3481 cacgatataa gaattggact aaaaacacct gaattagccc gtcccggaga atcttttacg
     3541 atcgattatc acacagacaa acccggtaag atcatcgtat ttgcagtgga tgaaggaata
     3601 ttgcaggttg ctaattatac aacacccgat ccactcagtt tctttttcca gaaacgagca
     3661 ttagaagtgc taactcaaca aacagttgat caaattctac ccaagtttat ccaggataga
     3721 gaaatatctg ctgttggagg tgatgagggt gaagcggctt tagcgagtcg tctcaatcca
     3781 ttcaagagaa agactgactt accagttgtt tactggtccg gtattcttga cacagacagt
     3841 gctgacaggc agttaactta tcaaatacct gattatttta acgggtccat tcgggtagtc
     3901 gctgtggcag ttgctaatga ctcagtggga tctcgcgaag tttcagcaga ggttcgcggt
     3961 gattttatta taagtcccaa tgtgcctact ttcgttgcac ccggagatga atttgaaata
     4021 tcagccactg tggcaaataa tattaaagat tcaggagaac atgctcaagt tgaagtagaa
     4081 cttaccgcat catcagagct tgaagcaatc ggtagtgcga aagagtctct ggaaatcagc
     4141 gaaggcaatg agcaaactgt gtattttaaa ttgcgcgcta aatcactgtt gggatcagca
     4201 aggattgcta tcactgccaa atcaggagat aaatcaagca agatggatag cacgctcagc
     4261 gtcaggcctg ccaatccctt cacaacatat attaatagtg gcaaatcaca agattctcaa
     4321 aaatcgttag aggttatcca gactttatat ccggaacatc gcaaggtaga agccactata
     4381 tctaccagtc cattagttct aatctttgga ttacaaagat atttggataa tttcccatat
     4441 ggttgtacgg agcaattgac aagcaaggcc ttgccctggc tggttatgaa cagtttaccc
     4501 tggttcagtt cggaaagtgg cccaattcac gaaaaaatta ataccacaat tcaaatgtta
     4561 agccaaagac aaatgtctaa tggcgggttt agctattggc ctggtctaag tgacaattca
     4621 gcgaatagtt ttgcatcagt ttatgccatg catttcttaa ctgaagcaag agcacaagga
     4681 ttcaacgtcc ccagagagat gttctacaat ggcattaatt accttaaaga attggctagt
     4741 cgcaatccca cagatgcaga ctcagcaaga attcaagcct acgctatcta tattttaacc
     4801 cgtaatgaat tagtcactac aaattattta gccaatttgc aattgtattt acagcaagat
     4861 tcttcaaaaa actggctaaa agacatcaca gcagtttaca ttgcagccac ctatcaatta
     4921 ttaaaaagtt atagtgaagc gaatcaattg attggccaat atcaaccacc aacaaataat
     4981 gcatttacga ctgattttta tgatcgcaat atcgcagatg cgcaatatct ctatcttgtt
     5041 gcaaaacatt ttcctaattt actgcctcaa gtaagcgaca atatactcat gaatttggta
     5101 gaagcgataa ataatgatga aatcaataca gttttatcag gattttctag cttggctttg
     5161 ggcgcttacc cacaaaatga atcagtagat ctatctgata agctgtccat aacagaagta
     5221 ctggcaaaca atcagcagag aaacatcccc tctgatcaaa ataattatca aaaattaaat
     5281 attgatgaaa ctgtcaaaac aatccgcttt aataatcctg acaaacaaac attcttttat
     5341 cagcttgttc agtcagggtt tgataaaata ataccgacag aacctttaaa gcaaggcata
     5401 gaaatctaca gagaatatcg ggatgagaaa ggcaatgtca ttgattcagc ggttttgggt
     5461 gaagaaattg aagtacacct gcaagtccgg tcattagata ataattatct gagcaatatt
     5521 gccattgaag atctcttgcc aggcgggttt gaggtcgtta gagactcagt aaaaacggac
     5581 aatatggatt atgccgatgt ccgagaagac agagttaact tcttcggctg ggtagattta
     5641 actgcaaaag aattagttta taaaataaaa gcgactaatg ttggtaaata catagttcca
     5701 ccagcctacg cagaagccat gtataaccca aatacgaagg caagaggttc ttcatcagtc
     5761 attacagtgt ccagagaaga agattaattg ctggcaagca aagtcagatt ttgattcttg
     5821 actttgctta ctaataaata ttaattgccc tcatatttgc tcatgccgac aaatgaagga
     5881 gatacttatc atagcctgag gatgagataa ggctctttcg tgaggaagca aacactattg
     5941 gatcttaggg aatagattcc tcagcccggc atagtaatga cttaagtttt gcattatata
     6001 gtaaagtatg gttttgtcct gtcgcaggga ggatctaata aaatgaacat tgttgctgga
     6061 tattctccaa ctctcgttgc ttcaattcca gttcctgcat acttaaatcc aactgctttt
     6121 caagctgtag agactttagc ctgagagcaa tatgctccct aaaagacaac caatcatctt
     6181 tataatctaa atagcgaaga ttacgatagg cttcagcttc cctgtggcta aattggttat
     6241 caaccatatc tggtaactga ttagcatcat ccaaagcatt gctaatgcca caacccaata
     6301 aaaaattggc ggacatcaaa gcatctccaa ccaagcaaaa cacacctccc tttggaagga
     6361 gcaaagcgaa tttatcagca atttcaatat ctgttttaaa aacagaagct aaaccagggg
     6421 cgctcgtctt aaaatccgta ttgttaaata ataattgcaa taccaagtca aaccattgtt
     6481 ttaactcttt tttattatta tccttgagaa tggattcagg aatttcccca gtgagataaa
     6541 cagctccatt cttttttgaa tatttcaaat aaattaaagg tggataatca tgcgtccagc
     6601 ccatggcctt taacggagta atcttatcta aagggatgaa gctggtcccc tcaattggac
     6661 ttaacaccgg attagctatc atcgccaaag gcggattatt gatcgacaga tacatcacac
     6721 caaaagcatt gtgatcagga ttatctaact tttcataacc aatcgcgaac tctggatttt
     6781 gcgaattcca caactgagta aatgattttg atgtttttcc ggatgcgtca actagcaaat
     6841 caaaactgtg attttgtatg ctgccattat gatccaaggt taactcctga ttctcagcat
     6901 caaccttgac aatttgcgtg tctcccaggt gaaaaatgag ctgctgctct gcattattga
     6961 attttacagt ataaggatct tcttcgtcct gaaaagtgaa accatctttg tcataaatat
     7021 atttcaactt actatgctga taacgctgca actctgcgat aggaattaca gtgcgctctt
     7081 tttcaattaa ttcaaaaaaa gctaaatcca attgatcttc tgcactcaat tgcaagttgg
     7141 attcatcgat gggacggatt aatttacaga taaaattatc tttgaattcc aaggaatacc
     7201 ctttgcttgc cagttgactt agagcaaaaa aatctctgat ggtatcatcc tttaagaata
     7261 ccccttgttt gcgactaaaa acatcatctt cagatcggcg gtcaattaat tcaacggaat
     7321 accctttagc taataatgca agtgcactag ccaagccagc cggccccata ccaatgatat
     7381 atgctttctt tcccataagt tggactctac tacttaaata aatgacaaat tttagcattt
     7441 aattgttaag aaattattag gtgagcattt ctattgccaa tgccccgctg tgcaggataa
     7501 aactcagata gactctcttt ttgtatagga agagtagcat gttgattatt aatttttctg
     7561 agctctgagc tggggggctc caacctggaa cccctaattt aggttcccaa atcgaaacga
     7621 attacatcca taattccttt ttgacaaata tcgcaaaatg atgcgtgaga ttccataaca
     7681 caattcaagc ctggtttatg acttgcaccc tgatgagatg gtgaatctcc atagccgcca
     7741 cgatatgccc catattgcgg aggggttgac tgccagtcac tatagggagt aggaatagac
     7801 atttgtggat tgactaactc attccatttt aatcccgaat agcttggagt agtcagaaaa
     7861 gtgatgtttt gtgaccacgg ttccttcatc tcaggggcaa attccaactc agtccgtcca
     7921 ccaccttcat attcttcatt gagcccaaaa aaatgaccca attcatgtat cagaatgtag
     7981 gtaaaataag ttgaattatc agcaggtatt gctgtgtgtg atttgtaatt acctacaccc
     8041 cagtactgac tgttattaac taatactatt ggataatcgt aaggtgctga cgccaatccc
     8101 tgacgaaact tgttttctcg tgtaggatat actatatgat accaacgtcc aaaattatcc
     8161 cagtatggat aatataaacc cagaaaagaa tcaaattctg gtactggtaa acctaaatct
     8221 tcagcgactc ccaaacgctt atgagatgga ttaaagactg cataaaaatc catataatcg
     8281 ataccaggaa accgttgact ttgtaatgcc tgcacagttt taatcgctcg attccaaaat
     8341 ttcatcttct cactttgagt atacccttca gcatatatat tcactcttaa agagggagtt
     8401 ttcactgcgg gtaatagttg cctcacttcc aaatcattga aatgacgatt atctcttgtt
     8461 aattcactag caatgatatt tttctcaaca accttttcca tgaaacctgt ttgagggttt
     8521 tctgcataaa gttcaaagat catatcatgg gagggaactg ggaaacgcaa attaatagca
     8581 cgtgccaact tgcgatattc cttaccagta ccaatcgcat cataatagac cgtattacca
     8641 ccgaatctgc tttttaaaat acccaaataa ctcccccatt tgggtttgtg aatacttcgt
     8701 gataataatg ccggtgttcc agaggactct attattactg taccaacctg ataagaatct
     8761 gtttcttcaa accaatcggt ttggattctt aatacatcaa tccctgcgtt ttcctgcaca
     8821 atcttcaaag aatcaggatt gatgtaatga aactttaaat ttgggtaatg actgtctctg
     8881 ggcactttat agcggtccca atagggattg gcgtatgcca gatgggaaac caaaaaaata
     8941 aagcaaatga taaaaaatct tatcatgggt atgacctcct tgtattccag aactactatt
     9001 ccatactaac ttgtagattt tcctacaaat gttaagtaag tttaacgaac actcttttat
     9061 ctatcgaact tgatatttcg ccagattgaa cgaaagcgtg ttaatttttg ggtatcgtaa
     9121 caacgattca caaaaaatca tgatttataa gacaaaagag tagaattacc catgtcacct
     9181 atccatggag gattatcacc taataaaaca gacggatcaa aacctaatga ttctattcta
     9241 acccactctt tattcaaata tttcaattga tcatttaatg ctttgttatc tgttttattt
     9301 gttgcatcat agcaacgatc aaaaggatcg taggtatagg gattacaaac taccgcctcc
     9361 attttatcca ttatcccaat tttgttatac caatcatcaa tcaacattgg accttctggt
     9421 ttggcagcgg gatcgagcac ataaatggta cccatgtagt tgacaacagc tgacacatga
     9481 taccaccaac ttactgtccc ctcagaagag tatggtgttt gcatttccaa atcaccaaaa
     9541 gcaaaaattt ttgctggaat aacaaaatgt tcactatcca gtttaatcct ggacagcgct
     9601 gctctggcaa agcaaccatc atcaggataa agccaactga ttcttctttg aaaatcaggt
     9661 ttatcaggtg aatacaaaaa acgagtatct cgtatcaggt taaacatgtt cgtcaactcg
     9721 tcatagctgc taacctttgg cacggtggag taatctattt tctgtaaaga gactttcttt
     9781 gaataaggag ttcccctggc aattaacatc ttcagttcat tttgtggttt attatttatg
     9841 atacgtttca ctgcatcctg ataagattct cctggaagcc gtttttgtga ggcagttgcg
     9901 tttgccaagc cgcaacaaat caaaaaagga aataatgata aaataatggc tatttttttc
     9961 atagcaatcc ttgagtattt ataatattgg ttgatatttt accaatattt gtcgtccttg
    10021 acattcttta taattagcat gaactgattg aaaagactag tctattagtc atatgaacta
    10081 tcaatcgtca aaacataatg gcttatattt tttaccaagg taagcattca agacgctgaa
    10141 tttaattgat ctgaccttac aataagtttc tcataataat gatctataat tcagatatac
    10201 atcaatttat aaattaaagg ctagtcatgg ctagtgataa ccctttttca ggcttaaggg
    10261 aaaaaatgtt aaatctctat catcaagcag aaagagacct gtttcgtgaa cctgtattta
    10321 gtaaccaatt tcaagatgct aagcttgatg atgttattat ttctcgagca ggtgtaaaat
    10381 ttctatgggg taaaatttca gaagaccagc gcgcacattt acaaggttta tttttagctt
    10441 atttagaaga tcaagcatcc agcatttata aagaatattt agataaaaaa ctaggcttag
    10501 gacctaacac cgccaccagc tcattgcttg ttcaatttga atcaaaaaaa attaaaagcc
    10561 ttgaaaatat tcgagcagtt ttggataaat gtgtaacaga aaacaaagaa gaggattatt
    10621 ggtttactaa tgctgcaaga ggaatcctac taactaacgc ttttgctatt tccgaaagct
    10681 caagtgaatc ctatcaatca gaattagaac agagaatcag caaactaatt ccacctcctg
    10741 gcaatgcaaa tgaggaagaa
//
LOCUS       DAXLDT010000104        10728 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXLDT010000104 DAXLDT010000000
VERSION     DAXLDT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.104"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            158..1012
                     /locus_tag="ACF2SP_002151"
     CDS             158..1012
                     /locus_tag="ACF2SP_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HFD2359927.1"
                     /translation="MKQTKLTLLGAGTMGSGIAQLFAQCGFYVTLIDNLQSQLDKAKA
                     TIAKNLHYLALTQNLESTHSIETILASITFTTKLDELKQSEYIIENITENWDRKKALY
                     QVLKKECSATCILGVNTSSIPITKIASLVDHPQRVIGVHFMNPAPMMPMVEVIKGYHT
                     DELTIEKTRTLLEQVHKKMIVVKDSVGFVSNRAMMIFINEAIFMVQENIASIEDIDVL
                     FKQCFGHKMGPLHTADLIGLDTVLYSLEIIYSELNDPKYRPCWLLRNMVDAGLLGQKT
                     KKGFYQYE"
     gene            1082..2209
                     /locus_tag="ACF2SP_002152"
     CDS             1082..2209
                     /locus_tag="ACF2SP_002152"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFD2359928.1"
                     /translation="MPNYQPQYVKQQRLFNQFIENCLHPHAKIFDKEQNIPRDFFYTL
                     AQNGFLGAGIPKQFGGQQWDELTIGIMHESFAKELCSLANILTVIGMVSKSLLQFGSK
                     AQKQIWLPRIATGETLAALALTESNIGSDLEHVETQLLDAGDEFILKGSKKYITLGQI
                     ADLFLVLANCQGELTAVLVEKDTPGLIITPMSNFLGLRSNMLAEIFFDNCRIPKTNLL
                     GNIGIGFNCVIQTALDEGRYTTAYGCVGLGQACLDAALGYTQKRKQFDRRLDEHQLIQ
                     KMITEMVVQIKAARELCFYAGELRQQKNPAYIAETLIAKYAASKMAVFVSNHALQLFG
                     AAGFEETYPVERYYRDAKVMEIIEGTSQMHEILISKLCNNK"
     gene            2226..3749
                     /locus_tag="ACF2SP_002153"
     CDS             2226..3749
                     /locus_tag="ACF2SP_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFD2359929.1"
                     /translation="MRSLDQYTTICSLFEEKVKQYPENTAVYLEDRQISFDVFNKQAN
                     QYARYLQRKGLPLNGIVGIQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQTILE
                     DSQIKFLIVSDHSLLTATPGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTG
                     RPKGVLISHQALLNRILWMQNTFPLQSRDVVFHKTHICFDISVWETCWWSIAGAGVVL
                     LPPRKEHDIKLFIQMIEQYQINVIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGE
                     ALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKRFDTVPIGKPIQNTQ
                     FFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPVLTHASFIDNPYLPGEKMYRTGDIV
                     RWNQEGELLFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMV
                     VFLIANNKQTKIGSAELKSFLKNRLPDYMIPEHYLWLSAFPIKENGKIDKEKLLSFI"
     gene            3919..5235
                     /locus_tag="ACF2SP_002154"
     CDS             3919..5235
                     /locus_tag="ACF2SP_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFD2359930.1"
                     /translation="MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSE
                     EQLHRSGISEQLIANPYYKPYRGILDDLESFDAAPFENTTKNFELLGVQGKVMSHLTY
                     RALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIA
                     PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYL
                     YEENGLFSSDGHCHSFDVSACGTLYSNGAGLVVLKRLEDAIEDNDLIHAVIIGSAMNN
                     DGNSSKEGFMAPGIHGQYTCIQSAWKNAGIEPGDLDYIAAHGASTKLGDATEIYALKK
                     AFKDVKKTHSCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAVVPSINFETPNPDFM
                     LEDSPIYIATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEN"
     gene            complement(5548..7029)
                     /locus_tag="ACF2SP_002155"
     CDS             complement(5548..7029)
                     /locus_tag="ACF2SP_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HFD2359931.1"
                     /translation="MSFFDLLINKANHLVNQIYKKNYDPITPDQLIWQHIQDCEKQFQ
                     KNQEAIYYSKDILQDLINKRLQTILLHAKTKSPWYKKTLAKINIENFTRERLEELPTI
                     NKNILMENWDAIVTDPKLSLNLVEKHLSRKNDSIDTLYLFSRYQVVSTGGSSGKRGVF
                     IYDWDEWITFHTAFVRYPLYNYERTQIVTTKLNTNPRIVSLFVTNTAIAAYSLAKTFG
                     RGNQNMYFLPMAVTPLNIVITRLNQIQPDILSAGPSYIHKVCQLVQKGQIKIEPKILF
                     VGGEPLFEQTLALIKETWPKVDIFNVFGCTEGMVGLNCRANVDEMHLNDDQCIIEPLD
                     EQNRPVDKGIMVSKMYITNLYNYTLPLIRYENSDEILFLNKTCECGIHHQLIQTPKGR
                     PGCDFNYPGNIFVHHSLFLGALLPEKNIQEYQVEQTIDGVNIKVVTSGYINKSELQKN
                     IRLRLSKVGLVDAQVNITEVPEIKYLYSGKLNRFIPLNSANQS"
     gene            7690..8733
                     /locus_tag="ACF2SP_002156"
     CDS             7690..8733
                     /locus_tag="ACF2SP_002156"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFD2359932.1"
                     /translation="MAYHQLSYTFLNHQKLPLIIQPQAKAEGNIDFLKQFLKEENALF
                     KEQLLNYGAIILRGFYVNTAEQFLEVIQSSDLGPNYSYDFCPIPRTQIRENIYTSINI
                     HPSFNLALHNEKSYDQDFPSHIFFNCIHAPQTGGDTALADGNKIWFSLPKLLQKKLQS
                     KGILYRRHYYGSGIQYKIIRSIGKNSGCMTWMERFQTDDQNKVEVMLQKMGVQFRWIQ
                     GNGLIIEQLLPACRNHPISGKLVWFNQSNHANHYYNGTSDYIKSKINNTFSRFILLHK
                     YFHPYIAFYGDGEPLSKQEADCINSAIQKNTVSTAWQPGDVMIVDNFSCLHGKTPHTG
                     NRLILVGLTKYFD"
     gene            8748..10640
                     /locus_tag="ACF2SP_002157"
     CDS             8748..10640
                     /locus_tag="ACF2SP_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HFD2359933.1"
                     /translation="MPDDISRKQFSIGDLYFTNELEVSGLIYEIFYQKSYLSDFLTLS
                     PGAVVFDVGANIGVFSLFVLKQCHYDTEIYSFEPVPATFKCLKKNLARFKHNVHVYNA
                     GIGNVPKDCSIDFTLFGESSVTATYKPTDKIISNYQPLLNYETLLKLSSFQNKPLYYQ
                     LKYLPFLRNYLIKKNYKHQTLETKVRCHLTALGRFIENNQIARIDLLKIDVEGAELDV
                     INSIKPEQFSLIKQLSIEVHDIDNRVEKLVSYLQKQGYITYVDRNPIFAELGFNHHMI
                     YAKLPEPAIMTLSEEPNNPENYIEARQYFYGLLAGGVRIKLLESMFDLDLFRLFTGKP
                     YLLENDIIKRLELKPVRAKKWLHLLCCEDFLKKIMVGSQVGYQLAKSQLMLGEGYWGF
                     KQYYDFYWQRMANEKLSNILRFTDPKFNVSWPPKTAEEANFLETWMTKTATPLIQTLL
                     ACLDLNQYRSILDVGGGDGTIACALAQAYPHLKITVYNLPESAKIAQKNIDAMGLQKR
                     ISVFSGDFINDEQFPAGFDLILFVRVLWDWDNSRKRKLLNMAYHALKRKGHVAICEGF
                     KELCYDLCLTWEYSYIFADGFDAEVFKTSDEYKIMLQQIGFTPIPIKSISPSEPVPGT
                     VVLAEK"
BASE COUNT         3333 a         1877 c         2137 g         3381 t
ORIGIN      
        1 gttaactact attgatgcta tcgctcactt tatttccagt aaactataat tgtaataggt
       61 cactccccta tgttggattt agggcaaaca gccagagttg agcttttact tataattcaa
      121 tacgaatagc tgtgataagc tttggtggac aaacggcatg aaacaaacaa aacttactct
      181 tcttggtgcg ggaacaatgg gttcaggtat tgcccaacta tttgcacaat gcggatttta
      241 tgtcacttta atcgataatc tccaatctca attggataaa gcaaaagcca ctatcgctaa
      301 aaatttacac tatctggcat taactcaaaa tttagaatcg acacattcta ttgaaactat
      361 cctagcatca attaccttta caacaaagtt agatgaactt aagcaatcgg aatatattat
      421 agaaaatatc actgagaatt gggatcggaa aaaagcgttg tatcaggtat taaaaaaaga
      481 atgtagcgca acctgtattt tgggggtaaa cacttcatcc atccctatta ctaaaatcgc
      541 gtcacttgtt gatcatcctc aacgcgttat aggtgttcac tttatgaatc cggcacccat
      601 gatgcctatg gtagaggtca ttaagggata tcacactgac gaattgacaa tagaaaaaac
      661 aagaacatta ctagaacaag tacataaaaa aatgatcgtt gttaaggact ctgttggttt
      721 tgtgagtaat cgggccatga tgatttttat taacgaagcc atctttatgg tacaggaaaa
      781 cattgcatca atcgaagaca tcgatgttct atttaagcaa tgctttgggc ataagatggg
      841 gccgctgcac acggctgatt taattggctt ggatacggtt ctctattctt tggaaatcat
      901 ctattccgaa ttaaacgatc cgaagtatcg accatgctgg ttattaagaa atatggtgga
      961 tgcagggctt cttgggcaaa aaacaaaaaa gggattttat caatatgaat agggggtagt
     1021 ttaccattcg ctatttcaac ccggatggtt aaattgtaaa caaacactag ggagagcaat
     1081 tttgccaaat taccagcctc aatacgtgaa gcaacagcgg ctatttaacc agtttataga
     1141 aaattgcctt cacccccatg caaaaatttt tgataaggaa caaaacattc cgcgcgactt
     1201 tttttataca ttagcccaaa atggtttttt gggcgctggt attcccaaac aatttggcgg
     1261 gcaacaatgg gatgaattga ccattggtat catgcatgaa tcttttgcta aagaactttg
     1321 ttctttggca aatatcctaa cagtaatagg catggtgagt aagtcacttt tgcaatttgg
     1381 ttcaaaagct cagaagcaaa tctggttgcc ccggattgcc acaggagaaa ctttggctgc
     1441 attggcttta actgaatcta atatcggtag tgatttggag catgttgaaa ctcagttgct
     1501 ggatgcaggt gatgaattca ttctaaaagg aagtaaaaaa tacattacct tagggcaaat
     1561 agctgatttg tttttggttt tagcaaattg ccagggcgaa cttactgcgg ttttagttga
     1621 aaaggacaca ccgggcttaa ttattacacc catgtctaat tttttaggat tacgttctaa
     1681 tatgttggca gaaatttttt ttgataattg ccgaattcct aaaacaaatc tgttgggtaa
     1741 tatcggtatc ggttttaact gtgttattca aactgctttg gatgaaggac gttatacaac
     1801 agcgtatgga tgtgttggtt tagggcaagc ttgcctggat gcggctcttg gctatactca
     1861 aaaaagaaaa caattcgatc gcagattaga tgaacatcag ctcattcaaa aaatgataac
     1921 tgaaatggtg gtacagatta aagccgcccg ggaattgtgt ttttatgccg gagaacttag
     1981 acagcaaaaa aatccagcat acatagctga aacattaatt gcaaaatatg cggcttcaaa
     2041 aatggccgtt tttgtatcta accatgcttt gcaattattt ggcgcagctg gctttgaaga
     2101 aacttatcct gttgagcgat attacagaga tgccaaggta atggagatca ttgaaggtac
     2161 ctcacaaatg cacgagatac tcatttcaaa gctttgcaac aataaataga ggatattatt
     2221 aaacaatgcg atcccttgat cagtacacga caatttgctc cttatttgag gagaaggtaa
     2281 agcaataccc ggaaaataca gcggtttatc tggaagatcg ccaaatttcg ttcgatgtct
     2341 ttaataaaca agccaatcaa tacgcgcggt atctgcaaag aaaagggctg ccattgaatg
     2401 gaattgtcgg tattcaactc gcgcgctctt ttgaaatgct ggtggcaatt tttgcagtat
     2461 taaaggcagg tggtgcctat ttgccgatag atcccatggc ccctgttcta agaaatcaaa
     2521 ctatccttga agacagccaa atcaaatttc taattgtcag tgatcattca ttattgacgg
     2581 caaccccggg tttgcaagtg ttaagcattc atgccaatgt gagcgatgaa gattgccata
     2641 atttaaacct ccctaggaaa aaacaggatt tagcgtatgt gatgtatact tcgggcacta
     2701 ctggcaggcc gaaaggagtc ctgatttcac atcaagcttt gcttaatcgt attttatgga
     2761 tgcaaaacac atttcccttg caaagtcgtg atgtagtatt tcataaaaca cacatttgtt
     2821 ttgacatctc tgtctgggag acctgttggt ggtctatcgc gggcgccgga gtagtacttt
     2881 taccgccaag aaaagaacat gacatcaagc tgtttattca aatgattgag caatatcaaa
     2941 tcaatgttat tcattttgta ccctccgtat tacgaatatt tttaagttac atcgaacagg
     3001 atttttcgat aaatcgctta agctctttaa agtacgtatt ttcaagtgga gaggcgctgg
     3061 atgccaaaag tgttaatcta ttcaaccagt tatttcaaga tcaaaaaaca atattagtca
     3121 atttatacgg gcccacagag gccacaatag atgtttcctg ttttatttgt gaaaagcaca
     3181 aacgttttga cactgtaccc attggaaagc ctatccaaaa tactcaattt tttgtgttag
     3241 acgagcagtt aaattcaacc ggcttcgaac ctggagagct ttatatttcc ggagttggat
     3301 tagctcaggg ttatttaaat aatccagtgc tgactcatgc atcgtttata gacaatcctt
     3361 acttgcctgg agaaaaaatg tatcgtactg gagatatagt ccgttggaat caagagggtg
     3421 agttattatt tttaggccgc aaggacgatc aggtcaaatt acgtggcata cgtattgaat
     3481 taggagaaat acagcatcat ttattagcgc atcccaacat ccatgatgcc gtcgtggtct
     3541 gtgagaaagt agattgtctt gatcatagaa tggttgtatt cctgattgct aataacaaac
     3601 aaacaaagat tggcagcgca gaactgaaat cctttttaaa aaacaggctc ccggattata
     3661 tgataccaga gcattatcta tggttaagtg catttccaat aaaagaaaat ggaaaaatag
     3721 acaaagagaa actattgagt ttcatttagc atgagcaatg cctgagaaat aaaaataatc
     3781 agggaaaatg acttatactt aattaaaagc cggttgcaga aataatataa actatgtttt
     3841 atgttatgaa cccgtcatac gtcgtttagt tagtgtttat taaattcttg gtgcggcgaa
     3901 gaaatggagc cataaattat gagtgacaat aactactcaa acaaaattgc tattgttggt
     3961 atggctgtaa aatttcctgg ggctaatgat cttgatgaat attgggattt attgaagttt
     4021 ggtaaagaag cagtcactaa attttccgaa gaacaattgc atcggtcagg tatttcagag
     4081 caattaatcg ctaatcccta ttacaaacca tacagaggga tattagatga cctggagtca
     4141 ttcgatgccg ctccatttga aaataccacc aaaaattttg aattgttggg tgtgcaaggc
     4201 aaagtgatgt cgcatttaac ttatcgggct ctgagcacca tgagaagtca ggaacatcat
     4261 tcaaataact acataaaaaa tacagctgtt tatattggcg ccaataatca acccgcctcc
     4321 catttcctgg gagccgccta ctaccaggcg attgatactc aaaaagcgat agaaaggtat
     4381 tattcaaaag tcattgcacc atatatttct tatcaatttg gatttcaggg tagttctatt
     4441 gatttatata cagcttgctc gtcttctctg acagccgtga tccaaagctg tagagagtta
     4501 aacagtcatc aatgtgatat ggcaattgca ggtgcgtgct taatcgattt accccaggaa
     4561 gttggatatc tttatgaaga gaatggcttg ttttcctccg atggacattg ccattctttt
     4621 gatgtttcag catgtggcac cttatattca aacggtgctg gattggttgt tctaaagcgg
     4681 cttgaagatg ccattgagga taatgatcta atccatgctg tgattatcgg atccgccatg
     4741 aataacgatg gtaattcttc aaaagaagga tttatggcgc cagggatcca tggccaatat
     4801 acttgcatac agtcggcttg gaaaaacgct gggattgagc caggtgatct ggattatatt
     4861 gcagctcatg gtgcttcaac caagttgggt gatgcaacag aaatatatgc cttaaaaaaa
     4921 gcattcaaag acgttaaaaa gacacattct tgtccaataa gctcagtaat tagtaatctg
     4981 gggcatactg gtattgtgtc cggtatggcg tcaatcatta aaactgtgct tatgctcaaa
     5041 aataaggctg tggtcccttc aattaatttt gaaaccccta acccagattt tatgttggaa
     5101 gactcgccta tatatattgc cactcaattt caggatttcc ctcaaattaa aaaacactat
     5161 ttggcgggtg tttccagtta tggcgcaggg ggaagtaaca cacaccttgt tttgcgtagc
     5221 tatgatgaaa actagggttc tgttgatatt tcgtttttat tcctgataaa agttggcgtc
     5281 ctgcggtttg ttcgttagag actcacatac aatttaaaca gcaaggagta tgtcaatttg
     5341 tttacgttcg gcctgaggag tggcgaaagc cacgtctcga aggccatcct tcacaggctt
     5401 cgagatgatg cagatgggga ttgggccaaa ctgatcttgg tatccaaatt gtatgaggtt
     5461 tcctcagcgg caaaccagag taaatgaaga aatggcggtt aaacgcgttc tcatcttagt
     5521 ttgccataga gaaaataatt tctgttacta tgattggtta gccgagttca atggtataaa
     5581 gcggtttagc ttgcccgaat acaaatattt aatttcaggt acctcagtga tattgacttg
     5641 ggcatccacc aaacccactt tgcttaatcg taagcgtata ttcttttgta attcgctctt
     5701 gtttatataa ccactggtca caaccttgat attaacccca tcaattgttt gttcgacctg
     5761 atattcctga atattttttt ctggcaagag tgctcctaag aaaagcgaat gatgtacaaa
     5821 aatattacca ggataattaa aatcacatcc aggccgacct ttaggggttt gtataagctg
     5881 atgatggatg ccacactcac aggttttatt taaaaataaa atttcatctg aattttcata
     5941 gcggattaat ggtaaagtat aattatataa attagttata tacattttgg agaccataat
     6001 tcctttgtca acggggcgat tttgttcatc cagaggttca ataatgcatt gatcatcatt
     6061 caagtgcatc tcatccacat tcgcccggca atttagccca accatccctt cagtacaacc
     6121 aaatacatta aaaatgtcca ctttaggcca ggtttctttg attaaagcca aggtttgctc
     6181 aaaaagcggt tctcctccaa caaaaagaat tttgggttca atttttattt gtcctttttg
     6241 aaccaattga catactttgt gaatatatga agggccggca cttaaaatat cgggctggat
     6301 ttgattgaga cgagtgataa ctatgtttaa tggggttaca gccataggca gaaaatacat
     6361 attttgatta cctcgcccaa aggtttttgc taaagaatag gctgcaattg ctgtatttgt
     6421 tacaaacagg ctgacaattc ttgggtttgt gtttagcttg gtagttacaa tttgcgtgcg
     6481 ttcataattg taaagtggat agcggacaaa tgcggtgtga aatgtaatcc attcatccca
     6541 atcataaata aatacgcctc gtttcccact gcttcctcca gttgacacga cctgatagcg
     6601 ggagaataaa tatagggtat cgatgctgtc attttttcta ctcagatgct tttcaaccag
     6661 atttaatgaa agtttgggat cagtgacaat tgcatcccaa ttttccatta aaatgttttt
     6721 atttatagta gggagttcct ctaatcgttc tctcgtaaag ttttctatgt tgatttttgc
     6781 cagtgttttt ttataccagg gggattttgt ttttgcgtga agtaatattg tttgcagtct
     6841 tttattgatt aggtcttgca gtatgtcttt agaataatag attgcttcct gatttttttg
     6901 aaactgtttc tcgcagtctt gaatatgttg ccatatcagt tgatctggcg ttatggggtc
     6961 ataattcttc ttatagattt ggttaaccaa gtgattggct ttgtttatta agaggtcaaa
     7021 aaaagacata ttactcccct ttttccccag ggcgctatcc ttacactata aggcatggtc
     7081 tttgagagaa cccataatta gtggtgatat tcctaaacgg gattttaatc aataggtagc
     7141 ctggattaat ttttgattgg cagtcaccat aagattaaaa tgcctaagaa gattatagct
     7201 taaaaccaag caggatattc tattatataa ggtagtgccg ttatcagtca tttttagtgc
     7261 agtttaatga tttatgcaat tgcaaggagt tctcatcgag gttagaaggt gatgatgtta
     7321 aagtcgatgc tatccacaga taatttgaca ggacttacga aaaaccccaa tcgctatgtt
     7381 aaaaaatttt tttaattcgg tcatttagtt tatctaaact ccctcattaa aaacattttt
     7441 tgctttgacc cttggagttt ttcgtaggtc ctgtttgagt gattcagcag gagcttcttt
     7501 ggcaaaatct ggtggatttt cattgcatag caaggtcagt gtctatacta tttaatatag
     7561 ctattcgtca aagatgatgc ggtaattttt gtaatatagg tgaaaaagag ggagccactt
     7621 atagaatcag gaaggaccta cactctgaaa gtctgatagt acacctccgg attgtcagtg
     7681 aggaatttta tggcatatca tcaactcagc tatacttttt tgaaccacca aaaattacct
     7741 ttaattattc agcctcaagc taaagcggaa ggtaatattg attttttaaa acaatttctt
     7801 aaagaagaaa atgccttgtt taaggagcaa cttctcaatt atggcgccat tatcttgcgt
     7861 gggttttatg ttaatacggc tgaacaattt ttagaagtga tccagtcaag cgatttgggg
     7921 ccaaattatt cttatgattt ttgcccgatt ccgcgaacac aaatcagaga aaatatttat
     7981 acttcgataa atatccatcc gagttttaat cttgctttac ataatgaaaa atcatatgac
     8041 caggattttc ccagtcacat ttttttcaat tgcattcatg ccccgcaaac cggcggagat
     8101 actgcattgg ctgatggaaa caagatatgg ttttctttac caaagttatt acaaaaaaaa
     8161 ttgcaatcta aaggaatatt atatcgaagg cattattacg gttctggaat acagtacaaa
     8221 ataattcgat caataggcaa aaattctggg tgcatgacct ggatggagcg attccaaacg
     8281 gatgaccaaa ataaagtgga agtgatgctt cagaaaatgg gggtgcaatt tcgatggatt
     8341 caaggcaatg ggctaatcat agaacaattg cttcctgcct gccggaatca cccgatcagc
     8401 ggaaaactag tctggtttaa tcaaagtaac catgctaatc attattataa tggaaccagt
     8461 gattacatca aaagtaagat taataacact ttttctcgtt ttatcttgtt acataaatat
     8521 tttcatcctt atattgcctt ttatggcgac ggtgaacctt tatcgaagca agaagcagat
     8581 tgcattaact ctgcaataca aaaaaatacg gtatccacag cctggcaacc aggggatgtt
     8641 atgatagttg ataatttttc ctgtttgcat ggaaaaacac cccatacagg aaaccgatta
     8701 atcctggttg gattgacaaa atattttgat taaagggacc cataccaatg cctgatgata
     8761 tttcacgaaa acaatttagt attggagatt tgtattttac taatgaattg gaagtcagtg
     8821 gtttgattta tgagattttc tatcagaaaa gctatttgtc ggattttctt actttaagtc
     8881 caggggctgt tgtttttgat gtcggggcga atattggtgt tttttcccta tttgttttaa
     8941 agcaatgtca ttatgatact gaaatttata gtttcgagcc tgtccctgct acttttaagt
     9001 gtttaaaaaa gaatttggcg cggtttaagc ataacgtgca tgtttacaat gccgggatag
     9061 gaaatgttcc aaaagattgt agtatcgatt ttaccctctt tggggaaagc tccgttaccg
     9121 ccacttacaa accaactgat aaaatcatct ccaattatca accattgtta aattatgaaa
     9181 cattgttaaa gctctcttcc ttccaaaata agccgcttta ttatcaatta aaatatttgc
     9241 cttttttgcg taattatctc attaaaaaaa actataaaca tcagacttta gaaaccaaag
     9301 tgagatgcca cctgactgca ttagggcgtt ttattgaaaa taatcagatc gctcgtatcg
     9361 atttactaaa aattgatgtg gaaggtgccg agcttgatgt aataaacagc ataaagccag
     9421 agcagttttc gcttattaaa caactaagca ttgaagttca tgatattgat aatcgggttg
     9481 aaaagctcgt ctcctatcta caaaagcaag gttatattac ttatgttgat agaaacccaa
     9541 tttttgcaga gctgggtttt aatcatcata tgatctatgc caaattgcct gaaccggcaa
     9601 ttatgacact aagtgaagaa ccaaataatc ctgagaatta tattgaagca cgccagtatt
     9661 tttatggact ccttgctggt ggggtgagga ttaaactgct ggaatctatg tttgatcttg
     9721 atttgtttcg tttgttcacc ggcaaacctt atttattgga aaatgacatt atcaagagac
     9781 ttgaactgaa acctgttcga gcaaagaaat ggctacattt gctttgttgt gaagattttt
     9841 taaaaaaaat catggttggt tcgcaagtcg ggtatcaatt ggcaaaaagt caattgatgt
     9901 taggagaggg gtattgggga tttaaacaat attatgattt ttattggcag agaatggcta
     9961 acgaaaaatt aagtaatata ttgcgtttta ccgatccaaa attcaatgtt tcctggcccc
    10021 caaaaactgc cgaggaagct aattttcttg aaacatggat gacaaaaaca gcaacaccac
    10081 ttatacaaac tttattggct tgtcttgatt taaatcaata tcgatccata ttggatgttg
    10141 ggggcgggga tggcactatt gcctgtgcac tggctcaggc ctaccctcat ttaaaaatta
    10201 ccgtttacaa tttacctgaa tcagcaaaaa tagctcaaaa aaatatagat gcaatgggat
    10261 tacagaagcg aattagtgta ttttcgggtg attttattaa cgatgaacaa tttcctgcag
    10321 gatttgattt gattttattc gttcgtgttt tgtgggattg ggataattcc agaaaaagga
    10381 aattattaaa tatggcgtat catgctttaa aaaggaaagg tcatgttgct atctgtgaag
    10441 gctttaaaga actttgctat gatctttgtc tgacatggga atacagctat atttttgcag
    10501 atggatttga tgcggaagtt tttaaaacgt cagatgagta caaaatcatg ctgcagcaaa
    10561 taggttttac tcctattcca atcaaatcca tttctccttc ggagccagtg cctgggacgg
    10621 ttgtattggc agaaaaatag ttttcatcag ccatactgtt tggcatagac ccatttaaca
    10681 caactaagag ctcaaggttt tcgtaatttt gaaaattata aaacgcgg
//
LOCUS       DAXLDT010000105        10399 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXLDT010000105 DAXLDT010000000
VERSION     DAXLDT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.105"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..685
                     /locus_tag="ACF2SP_002158"
     CDS             <1..685
                     /locus_tag="ACF2SP_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFD2359934.1"
                     /translation="RAISTSISRHQQFENEEETQVAIASILTYVGFSIAVISGLLVAG
                     FNFTGLAIIAGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINIDGVEGFVKKI
                     RVRSTQILTPVNEDIIIPNSDLITRRVVNYMLTDNYWRVNCEVSVAFGTNLNLVKDIL
                     LEIANKHDDVIKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNFSIEEA
                     FRKHDISIT"
     gene            858..3002
                     /locus_tag="ACF2SP_002159"
     CDS             858..3002
                     /locus_tag="ACF2SP_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2359935.1"
                     /translation="MIKKIHRWLPLLVLIGLSVLFFSLHLDKYLSFNALRENHALLIA
                     WTQTHYFIAPLIFIVFYTAAVAISIPGAVLFTLTGGFLFGVFWGVLFVVISATLGATI
                     LFFAVRTALGDWFAKKASGWIERMRRGFQHNAFSYLVTLRLIPLFPFWVVNIVPALLN
                     IRAKIFITATFLGIIPGTTVYVMVGNGLSQIFAANQTPNLGIIFEPQILGPLLALAVL
                     SLMPVLYQFSTRKHQNHHSFSDIGRVINCDLAIIGGGAGGLSLASGCSQLGLKVVLVE
                     SGEMGGDCLNYGCIPSKSLLAAAKTFYYAKHATHFGVQTEAIKINFQKVMQHVHQIID
                     NISEHDSVQRFESLGVQVIKQVGKFLNPDTLQAGDSIIKAKRFVIATGSSPFIPPIRG
                     LEAISYFTNETIFDLKEQPEHLIVIGGGPIGCELAQAFAMLGSKVTLLEGLNLLPKDD
                     PDCVAVLRTQMKSMNILIYEQIEITQINSHPDTGISVCFEFQNTQFTITASHLLIATG
                     RRANVKPLDLEKAGVKLTSKGVEVNKYLQTSNKKIYALGDVTGLYQFTHMASYQAGIV
                     LRNIVFKLPSKVDYRAIPWVTYTDPELAHVGIGVSDALKHPDTQIIEWPFVDNDRAQT
                     EHTLNGKIKIITDKKARILGVTIVGPHAGELILPWVMAIREKKTLRSFTDVIVPYPTL
                     SEISKRVAGSFYAPKLFSNKTRMLVRWLQKIG"
     gene            complement(3058..3768)
                     /locus_tag="ACF2SP_002160"
     CDS             complement(3058..3768)
                     /locus_tag="ACF2SP_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359936.1"
                     /translation="MIHLLINWMNSKLIMRVIILNTILSLFLGVVGSLSHATKSKNIN
                     QIDSLFNTVKISTILSKDNLISPYNYLLTQPLMTTTLEKYYQRTSRIKVITAFTNPHN
                     NTYSRIIILYVDRNKKRNNVDLAQKKNEIIPVELAAININFEELPEKLITDITHTNIP
                     FGKLLTQYHLKVFSKDRRYFSVACNKILASLIHCKPNKKLYGRTNVLARIDNKKWVAH
                     VIEILSDEKEVKYRLTLN"
     gene            complement(3846..4571)
                     /locus_tag="ACF2SP_002161"
     CDS             complement(3846..4571)
                     /locus_tag="ACF2SP_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HFD2359937.1"
                     /translation="MENLEHGHSQKEIKERLAQIPSQNYLRDWIYGGIDGVVTTFAIV
                     SGVVGGQLSSLVILILGFANLLADGFSMAASNYLGTKSEVDQFQRYKTIEEKHIDFIP
                     EGEKSEIKQIFQNKGLHGQALDQVVEEITANRALWIKTMLQEEYGLPATLRFPLKSAL
                     YTFSAFLLFGIIPLIPYVLVMNNSFIWSCFFTAITFFVIGSIKSHWSTKSWLYSGFET
                     LIIGTVTASLSYGIGLFLHYLLT"
     regulatory      4675..4768
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4843..5787
                     /locus_tag="ACF2SP_002162"
     CDS             4843..5787
                     /locus_tag="ACF2SP_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD2359938.1"
                     /translation="MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEP
                     ADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGIN
                     SFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIG
                     FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQ
                     RGAAYTTEKPEEAYELLCQAKPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYT
                     KHLKIIDEHFDISQCYTNRFLPDTPYSDLKPIACCLEN"
     gene            5790..6860
                     /locus_tag="ACF2SP_002163"
     CDS             5790..6860
                     /locus_tag="ACF2SP_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2359939.1"
                     /translation="MRAGIVGAGIMGRLLAFVLHNAGWQVSLFDRSNEIMNCSSAAAG
                     LLTPFSELDKSILLISNLGQESIKNCWPRIIRDLPEAVYFQQLGSLVLCHPGDQAEWI
                     HFSRRILSQLNQSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
                     YLDFVCIPHITNTPVLSLKPGEINTKEQRYHFDVVFDCRGLGAQNIFSDLRGIRGELI
                     WLHAPGVQLHRPIRLLHPRYSLYLVPRPGNLYLIGASEIEAEDFSPVSVRTTLELLTA
                     VYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLIAVNGLYRHGYLIAPALAEEI
                     LRGVTSNFRHLNYPELWESYCD"
     gene            6853..7056
                     /gene="thiS"
                     /locus_tag="ACF2SP_002164"
     CDS             6853..7056
                     /gene="thiS"
                     /locus_tag="ACF2SP_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFD2359940.1"
                     /translation="MINVYFNDKIYKIQPSLSLRAFLKQLNYTQQHFAVAINNHHVPH
                     KAYEATILQANDRVDILFPMQGG"
     gene            7059..7847
                     /locus_tag="ACF2SP_002165"
     CDS             7059..7847
                     /locus_tag="ACF2SP_002165"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFD2359941.1"
                     /translation="MWNLAGKELSSRLLLGTACYPSLEHMQQAILNSGTEVITISIKR
                     QTSAGLDGESFWQAVKKLDCHFLPNTAGCRNAEAAINTAEIARELFDTHWIKLEVIGD
                     DYNLQPEPFELIKATRILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
                     GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQAMEFGFDGVLLNTAVALANHPALM
                     ATAFRHAVIAGHQAFTGGMMSERNVAHPSTPLIDTPFWHQVNNL"
     gene            7844..9310
                     /gene="thiD"
                     /locus_tag="ACF2SP_002166"
     CDS             7844..9310
                     /gene="thiD"
                     /locus_tag="ACF2SP_002166"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFD2359942.1"
                     /translation="MKKPIVWTIAGIDSSGLAGVHADMETFSRLNVRACSVITAVTAQ
                     NAHSITAVEEISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVV
                     LDPIITSSSGTNLFFPDLQQHKKNLIQLFRYVTVITPNRIEAEAILNRSISSYQDIIN
                     AASDLLSLGAKQVLLKGGHLKDNLFSQDYWTDGKESFWIANHRFPETNYRGTGCVLSS
                     ALTACLALGYSIKDAIVIAKMYVNRGIRQSIEIDKDASQLYHDSWPEDEADLPYLSPT
                     PFIKPVPSFKKCSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVH
                     LANQYNAALFINDYWELAIHYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEV
                     ARAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLHYPLVAIGGITLKNLSD
                     VLKTKIDGVSVISAITKASAPLVAAKQFLTQMNESHNE"
     gene            9303..>10399
                     /locus_tag="ACF2SP_002167"
     CDS             9303..>10399
                     /locus_tag="ACF2SP_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFD2359943.1"
                     /translation="MNKSNLAMNELTRYSQQIKMEEIGLDGQEKLKNSRVLCIGLGGL
                     GSPLLLYLAAAGVGVLGIVDDDIIELSNLHRQILYNHTHINKKKAVTAKKQLLAINPL
                     IQVESYSSRLTEENAAELIMQYDIIADGTDNFYTRYLIHSICFKLEKPYVYASASGFQ
                     GYCSIFHGKKDPCLHCIFPVSANDINMPNCEGAGVIGTLPGMLGIIQATEIIKWILQI
                     GNPLKKRLLAINLLTMTFKNIIISRNPDCEFCIQNQSIRQLINPVNCSTQIEPCHHRI
                     TLDEFYQLFTNNKNIHLIDVRSTKEHQIQNIGGIVLPVDELPQRLHELNPNRLIILYC
                     QSGKRSKIALDILLKAGFASVKYIENGLKGVT"
BASE COUNT         3164 a         1894 c         2012 g         3329 t
ORIGIN      
        1 tcgcgccatt tcaacttcaa tcagtcgtca ccaacagttc gaaaatgaag aagaaactca
       61 ggtagctatt gcttccattt tgacttatgt tggatttagt attgctgtta tttcaggctt
      121 actcgtcgcg ggctttaatt ttactggact tgccattatc gctggagcac tttctgtagg
      181 aattggtctt ggactgcaaa gcatagttaa taattttgta tcaggtataa ttttgcttat
      241 tgagaaacca ataagacctg gagatcgaat taatatcgat ggtgttgaag gatttgtaaa
      301 aaaaatcaga gtcagatcaa cacaaattct cactccggta aatgaagata ttattattcc
      361 aaactctgat ttaattacac gccgtgttgt caattatatg ctgacagata attattggcg
      421 tgttaattgt gaggttagcg tggctttcgg gacaaacctg aatcttgtta aagacatcct
      481 tttggagatc gctaataagc atgatgatgt tataaaaagt ggtagaaaca aacctgttgt
      541 gttatttacc agttttgctg acagtgcatt aacttttcaa ctctggtgca tgattaaaga
      601 tgtaaataaa aaatcgacag tcaaaagtga attaaatttc tctattgagg aggcctttcg
      661 caaacatgac atttcaataa catagtctta atgtgatttt catatttgaa ttggagatta
      721 aatacttccc ctactctgga aaataatgct ttgtatcatt aaaccctatc gataattaag
      781 gtatgacctc aaagtaaatt cctgttagga tcttcatata atttatatca gcttatcggg
      841 ttaaaggaat aacatgaatg attaaaaaaa tacatcgttg gttgcctttg cttgtattaa
      901 tcgggctatc ggtacttttc ttcagcctac accttgataa atacttgagt tttaatgctt
      961 taagagaaaa tcatgcttta ctcattgcgt ggacccaaac tcattatttt attgcgccac
     1021 taatttttat tgttttttat accgctgctg tagccatctc tattccagga gcagttcttt
     1081 ttactttaac tggaggattt ttattcgggg tattctgggg cgtgttattt gtagtcatca
     1141 gtgctacgct tggggctacc atactttttt ttgcggttcg cactgctttg ggcgactggt
     1201 ttgccaaaaa agcatctgga tggatagagc gtatgcgtcg gggatttcag cataatgctt
     1261 tttcttattt agttacactg cgattaattc cattatttcc cttttgggta gttaatattg
     1321 tgccggctct tttaaatatt cgagcaaaaa tattcattac agcgactttt cttggcatca
     1381 ttccaggaac aacagtatat gtaatggttg gaaatggttt aagccaaata tttgcagcca
     1441 atcagacgcc taatcttggg attatatttg aaccgcaaat attaggtcca ctgctcgcgt
     1501 tggctgttct ctctctaatg ccagttcttt atcaattctc aactcgcaag catcagaatc
     1561 atcacagttt ctcagatatt ggtcgagtaa taaattgtga tttagccatt atcggtggtg
     1621 gagctggagg tttgagttta gcttctggtt gttcacaatt aggactcaaa gttgttttgg
     1681 tggagtcagg ggaaatggga ggagactgtt taaattacgg ttgtatccca tccaaatcct
     1741 tacttgctgc agccaagacg ttttattatg caaaacatgc aacacatttt ggagtacaaa
     1801 ctgaagcaat aaaaattaat tttcaaaaag taatgcaaca tgtgcatcaa attatagaca
     1861 acatttctga acacgattct gtccagcgtt ttgagtcgct aggagtacag gttattaaac
     1921 aagttgggaa atttttaaac cctgatacct tacaagctgg cgatagtatt attaaagcga
     1981 agcgtttcgt gattgcaaca ggttcatcac catttattcc acctattcgt ggattggaag
     2041 caatctctta ctttaccaat gaaacgattt ttgatttaaa ggaacaacct gagcacttga
     2101 ttgtcatcgg tggtggccct attggttgtg agcttgctca agcattcgct atgctcggtt
     2161 ctaaagttac tctgctcgaa ggattgaatc ttcttcccaa ggacgatcct gattgtgttg
     2221 ccgtccttcg cacacagatg aaatcgatga atatcctcat ttatgaacaa atcgagatta
     2281 cccaaataaa ctctcaccct gatacgggta tttccgtatg ttttgaattt caaaacacac
     2341 aattcacaat tacagccagt catctgctca ttgcaactgg acgtcgggcg aatgtaaagc
     2401 cccttgattt ggaaaaagca ggggttaaac tcaccagcaa aggtgtcgaa gtaaataaat
     2461 acttacaaac cagtaataaa aaaatctatg ctctgggtga tgtaacaggg ctttatcagt
     2521 ttactcatat ggcgagttat caggccggta tagtccttcg taatattgtt tttaaactac
     2581 cgtctaaagt cgattataga gcgattccat gggtcactta tactgatccc gaattagctc
     2641 atgttggaat aggagtcagt gatgccttga agcaccctga tacccaaata atcgaatggc
     2701 catttgttga taatgatcga gcacaaactg agcatacatt aaatggaaaa ataaaaatca
     2761 tcacggacaa gaaagcgagg atccttggag tcaccatagt tgggccacat gctggagaac
     2821 tcattttacc ttgggttatg gcgattcgtg agaaaaaaac tctccgaagt tttactgatg
     2881 taatcgttcc ttaccctact ctgagtgaaa tcagcaaacg agttgctggt agtttttatg
     2941 cacctaaatt attttccaat aaaacacgaa tgttagttcg ctggttgcaa aaaattggat
     3001 aagtagattt ttaactaact atatgttttt tggctgaaag aaaaaattac aagctattta
     3061 attcaatgtt aaacgatatt tgacttcttt ttcatcggat aaaatttcaa taacatgggc
     3121 aacccatttc ttattgtcta ttctagcaag cacattggtg cggccatata attttttatt
     3181 tggtttgcaa tggattagtg aagccaatat tttgttgcaa gcaaccgaga aatagcggcg
     3241 gtctttactg aaaactttca gatgatattg agttaataat tttccaaaag gtatattagt
     3301 gtgagttata tcagtaataa gtttctccgg cagttcttca aaattaatgt taatagctgc
     3361 taattcaact ggaatgattt catttttctt ttgggcgaga tctacattat ttcttttctt
     3421 atttcggtct acatacagga tgataattcg agaataggta ttattatgcg gatttgtaaa
     3481 tgcagtaatt actttaatcc tggaggtgcg ctgataatat ttttccaaag tggtcgtcat
     3541 taatggctgt gtaagtaagt aattataagg agagattaga ttgtctttgc ttaatatagt
     3601 acttatttta acagtattga acagactatc tatctggttt atatttttgg atttagtcgc
     3661 atgagataaa ctccctacca ctcctaaaaa aagagataaa atggtattga gaataattac
     3721 tcgcatgatg agttttgaat tcatccaatt gattaataaa tgaatcatct ccaatattac
     3781 cccatgtaat taactacaaa atttatatct aacaagtacc gctaatagca gagctatgtt
     3841 ttattttaag tcagaagata atgtaaaaat agtccaatac cataagagag agaggctgtt
     3901 acagtgccaa ttattagtgt ctcaaaacct gaatataacc aggatttggt tgaccagtgg
     3961 cttttgattg aaccgatgac aaaaaatgta attgcagtga aaaaacaaga ccagatgaac
     4021 gaattgttca tgaccaacac gtaagggatt aatggaataa ttccaaaaag tagaaacgca
     4081 ctaaaagtat aaagcgcaga tttaagcggg aatcgaagtg tagcaggcaa cccatattct
     4141 tcttgtaaca tggtttttat ccataaagct ctgttcgctg taatttcctc aacaacttga
     4201 tctaatgctt ggccatgcaa acctttattt tgaaaaattt gttttatttc gcttttttca
     4261 ccttctggaa taaaatcaat atgtttttct tcaattgttt tatagcgttg aaattgatcg
     4321 acctcagatt tggtacccag ataattactc gctgccattg aaaaaccatc agcaagcaag
     4381 ttggcaaaac ctaaaattaa aataactaaa gaagataatt gtccacccac aacaccggaa
     4441 acaatggcaa aagtagtgac aacaccatca attcctccat atatccaatc tcgcaagtaa
     4501 ttttgagatg ggatctgtgc taatcgctct ttgatttctt tttgcgaatg tccatgctct
     4561 aaattttcca tagcagatcc tttctccata tgtgttattt attaattatc aattctgcca
     4621 atattatcgc tagtttgaca aagcatataa taaaatttag tatatttgag tttgttagat
     4681 ggcgcggggt gtcgggaaat ccggctgaga gttacccgtt gaacttgatc tgaatcatat
     4741 cagcgtaagg acgccttcaa aaaaaccatg gcctttttaa ggcatggttc ccttttgcca
     4801 tctccataat atttaaaaat aataacataa ggagttatgg cgatgtcatc actaaaatcc
     4861 agggttaccc tactgcttaa ttggtacact aacccttatc atacccccat acttgtggca
     4921 caacaattgg ggttttattc agaagaagat atcaagcttg ccattctgga accagcggat
     4981 cccagtgatg taaccgaaat cgttggcctg ggaacagtag attttggtgt taaagcaatg
     5041 attcataccg ttgcagccaa agcaaaaggt tatccagtta cttccatagg aacgcttctt
     5101 gatgagccac ctaccggact tattgcttta aaatcaagtg gcatcaattc tttccaggat
     5161 attgttggga aacgagtagg ttatatcggc gaattcggca aaaaaattat tgatgattta
     5221 gccagccttg cgggtattga tccgatgagc tacaaaacag tccgtattgg catgaatgtc
     5281 acagatgcca tttatcggga tgttatcgac actggtattg gttttattaa ttttcaaaaa
     5341 gttgaactgg aacatctttg cggagaaact gtatttttac gaatcgatca attagctggt
     5401 ttaggctgtt gttgtttctg ctcaatacaa tttattgttc ctgaaacaac attacagcaa
     5461 cccgaattag tcaaaggatt tctcagagca acacagcgcg gtgcagccta cacgacagaa
     5521 aagcctgaag aagcttatga gctattatgt caggctaagc cacagttgcg tactccttta
     5581 tatcaaaaaa tttttacaag aacattacca tttttttcac gtacattaat taatgttgac
     5641 agagattggg ataaggttgg acgctatacc aagcatctaa aaattattga tgaacatttt
     5701 gatatctcgc aatgctatac gaatcgtttt ttgccagata caccttattc tgatttgaaa
     5761 ccaatcgcct gttgtcttga aaattaatta tgcgagcagg cattgtaggt gcagggatca
     5821 tggggcgtct gctggctttt gtattacata atgcaggctg gcaggtatcc ctttttgatc
     5881 gctctaatga aatcatgaat tgcagcagtg ctgctgcagg tctattaaca ccgttttctg
     5941 aattagataa atcaattcta ttgatttcca acttgggtca agaatctatt aagaactgtt
     6001 ggccacgtat tattagggat ttgcctgaag ctgtttattt tcaacaattg ggcagtctgg
     6061 tgctatgcca tccaggagat caggcggaat ggattcactt tagtcgccgt atcctgagtc
     6121 aactcaatca gagtaattct gtttttcaaa aattatccca tgaaaatctc attgcgcttg
     6181 agcctgagtt gacaaagttt gaaactgctt attattttcc aaatgaagcg catattgatt
     6241 gccaaacact aatgttgtct ttaaaaaatt atctcgactt tgtttgcata cctcacatta
     6301 ccaatactcc tgtcttatcg cttaaacctg gagagatcaa tacaaaagaa caaagatatc
     6361 attttgatgt cgttttcgat tgtcgtggtt taggtgcgca aaatatattt tctgatttac
     6421 gaggtattag aggtgaactc atttggcttc atgcgccagg agttcaacta catcggccta
     6481 ttcgtcttct gcatccaagg tacagtctat atcttgtacc cagacctgga aatctttacc
     6541 tcataggcgc tagcgaaatt gaggcagaag attttagtcc ggtttcagta cgtacgactt
     6601 tggagttatt gaccgctgtg tattatttac atgggaactt tgatgaagca cgcatcataa
     6661 aaacagtgac tcattgccgt ccaactttag caaatcattt accacgtatt aaatttgccg
     6721 acgggctaat agcagttaac ggtctttatc gtcatggtta tttaatagcc ccagccttgg
     6781 cagaagaaat tttacgaggt gttacatcca atttccgtca tctgaattat ccagaacttt
     6841 gggagagtta ttgtgattaa tgtctatttc aacgataaaa tttataaaat ccaaccctca
     6901 ttatcattaa gagcattttt aaagcagctt aattataccc aacagcattt tgctgtcgct
     6961 ataaataatc atcatgtacc acataaagct tatgaagcta caattttgca agccaatgac
     7021 cgcgtcgata tcctctttcc catgcaagga gggtaatgat gtggaatttg gcaggaaaag
     7081 aattaagtag ccgcttactc ttaggtactg cgtgttatcc ttcgttagag catatgcagc
     7141 aggcaattct taattcgggt acagaagtta ttacgatatc aataaaacgc cagacttctg
     7201 caggattaga tggagaatct ttttggcaag cagtaaaaaa attagattgt cattttttgc
     7261 ctaatacggc tggctgccgt aatgcggagg cagcgatcaa tacagctgaa attgcgaggg
     7321 agttatttga tactcactgg ataaaactgg aagtgatagg cgatgattat aatttacagc
     7381 ctgaaccatt tgagttaatc aaggcaacca gaattttgat tgacagagga tttgaagttt
     7441 ttccttattg cacagatgat ctggttttat gtcaaaaact ggttgatgca ggttgtaaaa
     7501 ttttaatgcc ctggggggct cctataggct caggtaaggg tttaattaac ccctatgcct
     7561 tggaaacttt gcgttatcga tttcctgaca tcacattaat cattgatgcc gggattggta
     7621 agccatctca tgctgtacag gcgatggaat ttggttttga tggcgtgctt ctaaatactg
     7681 ctgtagctct tgccaatcat cctgctctca tggcaaccgc attccgccat gccgttattg
     7741 caggtcatca ggcatttaca ggaggaatga tgtctgagag aaatgtagct cacccaagta
     7801 ctccattaat cgatactcca ttttggcatc aggtaaacaa tttatgaaaa aaccgattgt
     7861 ttggacaatt gctggtattg actcatccgg attagctggt gtccatgctg acatggaaac
     7921 ttttagtcgc ttgaatgtca gggcttgttc agtcatcacc gcagtaactg cacaaaatgc
     7981 tcattcaatt acagcagtag aagaaatatc cagagatcag gttgctgctc agtgccgtgc
     8041 cttggaatta aatttaaaac cggatgccat taaaattggt atgttatgtt ctacaccaat
     8101 ctgtgaagaa atagcttatt ttttaaaagg ttatgaagga tttgttgttc ttgatccgat
     8161 aattacctcc tcttcgggaa ctaacctttt ttttccagat ttacaacagc ataaaaaaaa
     8221 tctaattcaa ttgtttcggt acgttactgt tattactcct aatcggattg aagcagaagc
     8281 tattttgaat cggagcattt cttcttatca ggatataatc aatgctgctt cagatctgtt
     8341 gtcactggga gcaaagcagg tattactgaa aggaggacat ctaaaagata atttattcag
     8401 tcaagattac tggaccgatg gaaaagagtc attttggatt gctaatcatc gctttcctga
     8461 aacaaattac cgcgggacag gctgtgttct atcttctgca ctcacagctt gtcttgcact
     8521 gggctattca ataaaagatg ctattgttat tgctaaaatg tatgtcaatc gtggcatacg
     8581 tcaatcgata gaaattgata aagatgcctc gcaactttat catgatagtt ggccagaaga
     8641 tgaggctgat ctcccctatt tatcgccaac accgttcatt aaaccagttc cctcatttaa
     8701 aaaatgttct atgggttttt atcccattgt tgatagtagc cactggttag aaatgctttt
     8761 gccattggga attaagtgca ttcagttacg aataaaggaa gcctctcaag agcggttgga
     8821 agaagagata aaacggagtg tccatttggc aaatcagtat aatgctgcat tatttattaa
     8881 tgattactgg gaacttgcta ttcattatgg tgctgcagga gttcaccttg gccaagagga
     8941 tctggaaaaa gctgatgtcg atagaattaa tcgatcaggt ttattcttag gtataagtac
     9001 gcattgctat tacgaagttg ctcgggctca tgctcttaac ccttcctacg ttgcttgcgg
     9061 ccccatatat gaaacaacca gcaaaataat gccatttcaa gcacaaggaa ttgcacgact
     9121 tgaacgttgg cgaaaaacgc ttcattatcc attggtcgcc attggaggta taactttaaa
     9181 gaacctttct gatgtcttaa aaacaaaaat tgatggtgtg tcagtgatct ctgctattac
     9241 aaaagcatca gcacctttag ttgcagccaa acaattttta actcaaatga atgaatcaca
     9301 taatgaataa atcaaatttg gcaatgaacg aattaacccg ctattctcaa caaataaaga
     9361 tggaagaaat tggattagac ggacaggaaa aacttaagaa ttctcgagta ttatgcattg
     9421 ggcttggcgg tttgggttcg cctctcttac tttaccttgc tgcagcaggt gtcggagtat
     9481 taggaattgt cgatgatgac atcattgaac taagtaattt gcataggcag attctttata
     9541 atcataccca tattaataaa aaaaaggccg ttactgcaaa aaagcaactt ctggctatta
     9601 atcctctgat tcaagttgaa tcttactcca gcagattaac agaagaaaat gcggctgagt
     9661 taattatgca gtatgacatc atcgcagatg gaaccgataa tttttatacg cgatatttga
     9721 ttcacagcat ttgctttaag ctggaaaaac cttatgtgta tgctagtgcc tcaggtttcc
     9781 aagggtattg ctctatattc cacggtaaaa aagatccctg cctccattgt atcttcccgg
     9841 tttctgcaaa cgatatcaat atgcccaatt gcgagggagc cggggtaatt ggtactttac
     9901 ccgggatgct aggtattatt caggcaacag aaataatcaa atggatacta caaattggca
     9961 atccattaaa aaagcgtttg cttgctatta acttgttaac aatgaccttt aagaacatca
    10021 ttatttcaag aaatccggat tgtgaattct gcattcagaa tcagtcaatt agacaattaa
    10081 ttaatcctgt taattgttca actcaaattg agccatgcca tcatagaata actcttgatg
    10141 agttttatca attatttacg aataataaaa acattcatct aattgatgtt cgtagtacaa
    10201 aggaacatca aatacaaaat ataggaggta ttgtgcttcc tgtagatgag ttgcctcaac
    10261 gcttacatga actaaaccct aatcggctta ttattctgta ctgccagtcg ggaaaacgaa
    10321 gcaagatagc attagatata cttctcaagg ctggctttgc ttctgttaaa tatatagaaa
    10381 atggtttaaa gggagtaac
//
LOCUS       DAXLDT010000106        10380 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXLDT010000106 DAXLDT010000000
VERSION     DAXLDT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.106"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1113)
                     /locus_tag="ACF2SP_002168"
     CDS             complement(<1..1113)
                     /locus_tag="ACF2SP_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFD2359944.1"
                     /translation="MPNYNLILNLPGFSIVKVSGYQPLLLDLTYNRLPRCAHCTSKAV
                     RKKSSYIRTVAHELIGHRRSLLRFKAYKLYCNTCNRYGNQQFPGINKHQRSTWRLQAA
                     VFHEHTHGISQKELSKRFNKGKATIERWYHRHYQEQARELMNTSCPVVLGIDEHFFSR
                     KQGFATTLCDLKKHKIFDIVKGRSEVDLSSYLSSLIGKERVKVVCMDLSSTYRAIVKK
                     HFPNAKIVADRFHVIRLLQHQCMMTYRELSGKIKSNRGILALLRTRPDRLTPQRRLKR
                     DAFLEDNPAIKAIYQFQQQVHALFLHKAMNKDWCKKMIPQFLNILDDLKNSPFKALAA
                     LGKTFCQWKEEIVRMWRFRKSNGITEGFHRKMKALPQ"
     gene            complement(1178..2473)
                     /gene="legL2"
                     /locus_tag="ACF2SP_002169"
     CDS             complement(1178..2473)
                     /gene="legL2"
                     /locus_tag="ACF2SP_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL2"
                     /protein_id="HFD2359945.1"
                     /translation="MRVELFNPSIKKIIVKLDQLKGLWIVQLNLSKNRLSEKKFTDLA
                     VLLSMLKAFGVTYLNLHFNNLGQKTGTELVALFKILKDSGISRLSLSANELYQKTGTE
                     LATLLTVLSDSDIIQLDLSCNWLGQRPDAELIELFKAWSSTPIIDLDLTNNDLGKKSG
                     IVLSEMFRALKVSSISHLGLRNNDLYQRTGTEFSSLLSAIKDSPITHLDLSDNGLHLM
                     VVTAFETLFDTLKDSSVTHLNLCRNGIGYLDMLEMTAMFCSLQCTGVTHLDLGENSLE
                     LMTAEELAAIFRVFKDSRLIHLGLNDSYLNNRTTEDLHLIFNALPANIATIKLSLEEL
                     TEMSSEQRQAIQRRFPRAEQVILVNNVEGRELNPSLSLVDANVYRRLGFVGVSPPSLA
                     GLTSLFVVGNNLRFTREVQKLLPDEVTERIVKNIRLYPN"
     gene            2947..4371
                     /gene="plaB"
                     /locus_tag="ACF2SP_002170"
     CDS             2947..4371
                     /gene="plaB"
                     /locus_tag="ACF2SP_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_341574636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase PlaB"
                     /protein_id="HFD2359946.1"
                     /translation="MIVIFVHGWSVTHTNTYGELPQWLENQSKQGKLDIQVGNIYLGR
                     YISFDDTVTVDDIARAFDQAVRDEIADKLRDGQRFACITHSTGGPIVRKWMDLYFKNN
                     LAKCPLSHLIMLAPANHGSALAQLGKSRLGRIKSFFEGIEPGKCVLDWLELGSDKSWQ
                     LNESWLDYDCTANGVYSFVLTGQKIDRQFYDAVNSYTGESGSDGVVRVAATNMNYSLL
                     KLHQEGDNGESLVVAKMTRTQPMAFGVLPGLSHSGKNIGIIRSITMANAANHPTAIWI
                     LRCLQVKSRDSYNKLVKELDNITKETQKNEHKEFVKTLVFTREYITNRYSMIIFRLID
                     DRGNHLIDYDLYLTAGPQYSEQALPAGFFVDRQRNLNNRGKLTYFLDYDIMEGGINTP
                     KMQGNLGFRVKAYPESSDQALAYYRLLDFHSSLADIHKILHPNETVMVEIMLQRRVDR
                     TVFRISNNLTPAKISGKPTGKKID"
     gene            complement(4443..5120)
                     /locus_tag="ACF2SP_002171"
     CDS             complement(4443..5120)
                     /locus_tag="ACF2SP_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HFD2359947.1"
                     /translation="MYSYIDFNDKVHRLIPSKFPPVTLFDWADSAEELEQIALLEGLT
                     NERILAEFGKINLIDQEDWIGGPGSTPIMAAFTHTGFESRFSDGSFGIYYAASSLETA
                     IKETCFHRERFYSASNEKPCSISMREYVANIKKPLIDITDKSYKDLFNPDPACYSKSQ
                     EFGKKICEKKEWGLLYPSVRDLNGLCVAVLRPPALTIPIQGCHLRYIWDGEKISDIYK
                     ESKITNI"
     gene            complement(5122..5496)
                     /locus_tag="ACF2SP_002172"
     CDS             complement(5122..5496)
                     /locus_tag="ACF2SP_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HFD2359948.1"
                     /translation="MQTNTRSLQSIPDEVVWKSLTNLVERFDLKESEARILMGDMPRS
                     TYTSHKAKLNRDQKERVSYLLGIYKSLRILFEDGEQARTWINRKNDLPPFKGLTPKEY
                     MLEGGMVRLADVRKFLDFWRGY"
     gene            5709..6368
                     /locus_tag="ACF2SP_002173"
     CDS             5709..6368
                     /locus_tag="ACF2SP_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFD2359949.1"
                     /translation="MIDTIQSEPKINFTYNYKQPDDYRFSLDSIHLAKFVAKQLESYP
                     DLGSLRVLDLCAGCGVIGIELSWYLQAIRQIDFIEIQDIYTEYFYQNIANVNRPELQF
                     RWHLLNYDELHKKKWEDKFDLIISNPPYFQPGHGMLSPSKFKNRCRFYLDSSFQSYIQ
                     ALGNSLANRGKAYFLLRPLKHHGLDLFSDIQKILQETSVTATKISHIRGTDIILLEKL
                     K"
     gene            6606..8921
                     /locus_tag="ACF2SP_002174"
     CDS             6606..8921
                     /locus_tag="ACF2SP_002174"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFD2359950.1"
                     /translation="MKENVSHLPRELSRMVFWSLTCLKKAIAEVYGKETYERIEQIRL
                     SMQDTIGSESFSLGKALHSLQIELSKLEKKQLYQIAHGFSLMMELINTCENAYRIFRI
                     NQRKEQVYSDMPQGIHYVLTAHPTEARTNEFLKLFKEIRDYLIEVLKSPYLMNESHLD
                     QMLKISLQINISYHEKPSVEDEAQYIYQYALHPLNIKLYHSFLAKGVPIQLRTWVGGD
                     KDGHSGVNEKIMLKSLELSRSFFIAYIQECLREVLEIIKLSTSLDKNKPLADQINQLI
                     KKAYGLKKIQSKEHEKISIFKNELTATQINTLKFLNFEPEQFSIISSIFHLYPALVMP
                     IELREDSAVVKESLTSIKKTTITKMLEQVHSITHGTDPKNYIRGFILSMVESAEDIKN
                     GINLVKKVFKSYALPVVPLFENQLALTNADTILNNSITEAIRQKHLKQWDGNYEVMLG
                     YSDSSKENGVLPSRLMIAKSLKNIQSTLENKNLRPIFFHGSGGSIERGGGDIKEQTAS
                     WSKEIMSNYKVTVQGEMVARLFGSSSILKSQIDKFLDIYSQKGKSNDNDYPEELSLFS
                     TRVSQKYKTLIQSDWFWQIVEQASPYHFLKELKIGSRPTKRKSGPDQRKLRAIPWILC
                     WTQTRLLFPTWWGIGSTWSELNGIEKNRLKALYKENALFAAFVKQLGVTLSKVYLPVW
                     EQYLLQLTGSIEYLRPFQSELESTIQFFYQITGEKDFCFHQPWLGESIQLRSTLIHPL
                     NLIQIEAMKRKDLPLLRKTVTGISCGMLTTG"
     gene            9074..9556
                     /locus_tag="ACF2SP_002175"
     CDS             9074..9556
                     /locus_tag="ACF2SP_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFD2359951.1"
                     /translation="MSFSMKINNIPYYVATRVVKRIQSWLGLNTLGARAIVTNTEGHV
                     LLVKHTYQPHWYLPGGGVKKGESTKAAVIRELHEEVGLVVAEQDVILFGIYHHKYLGV
                     NDYPVIYIVKNFTSHVTHSGEIEQMGWFSLDALPEMVSPGTKRRLGEYFDNNPISEKW
                     "
     gene            complement(9607..>10380)
                     /locus_tag="ACF2SP_002176"
     CDS             complement(9607..>10380)
                     /locus_tag="ACF2SP_002176"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFD2359952.1"
                     /translation="NTLKNFNINTDIVCLLPPERALITPLLNATGLVDLCIPRGSQNL
                     INFVRDNAKIPVIETGAGIVHTYFDKSGDLEKGKKIINNAKTRRVSVCNALDTLIIHA
                     DRLKDLPELVETLSQKNVIIYADQDAYQVLDKNYPEQLLMKAKPQDFGHEFLDYKLAI
                     KTVPNIKAAIDHIQQFSSYHSEAVIAEDESAIDKFLTEVDAAAVYANASTAFTDGGEF
                     GLGAEIGISTQKVHARGPMGLEALTSYKWVIRGTGQIRD"
BASE COUNT         3293 a         2034 c         1898 g         3155 t
ORIGIN      
        1 ttggggtaga gccttcatct ttctgtgaaa gccttctgta atgccattag attttctaaa
       61 ccgccacata cgaacaatct cttctttcca ttgacagaat gtttttccaa gagctgccaa
      121 ggctttaaag ggactatttt ttaagtcatc aagaatattt aaaaactgag gaatcatttt
      181 tttacaccaa tctttattca tagctttatg taagaataat gcgtgaactt gttgctggaa
      241 ctgatatata gcttttatag ccggattatc ctcaaggaat gcatcgcgct taagtcgccg
      301 ttgaggggtt aatctatccg gtcgcgtacg cagtaaagcc aatatgcccc ggttactttt
      361 tatttttcca gatagctcgc ggtaggtcat catacattga tgttgaagca agcgaatgac
      421 atgaaaacgg tcggccacaa tttttgcatt gggaaaatgt tttttaacaa tggctcggta
      481 ggtactgctt aaatccatac agactacttt gactcgctct ttccctatca gagaggacag
      541 ataactcgaa aggtcgactt cacttcgtcc tttcacgatg tcaaaaatct tatgcttttt
      601 aaggtcgcat agtgttgttg caaatccctg ttttcggcta aagaagtgtt catcgatacc
      661 aagcacaacg ggacaagagg tattcatgag ctcccttgcc tgttcctgat aatggcgatg
      721 ataccaacgc tcaatggtcg cttttccttt gttaaatcgc tttgaaagct ctttttgaga
      781 aatgccatgg gtatgttcat gaaacacggc agcctgtaaa cgccaggtag acctctgatg
      841 tttattaata ccaggaaact gttgattacc gtaccgatta cacgtattac agtaaagctt
      901 ataagccttg aaacgtaaaa gactacgacg atgacctatt aattcatgcg ccactgttcg
      961 aatataactc gatttcttgc gaactgcttt gctggtgcaa tgggcacacc ttggcaaacg
     1021 attgtaggta aggtctaata gaagtggctg atatccactc acttttacta tagaaaatcc
     1081 aggtaaattt aggataagat tatagttagg cactttaatt tccttttgat cgctaaatca
     1141 aggacttagt ctagactaat ttaattaaag tgccccctta attggggtag agccgaatat
     1201 tcttgacaat gcgttctgtt acttcatccg ggagtaactt ttgcacttca cgcgtgaacc
     1261 tgaggttgtt gccgaccaca aacaaggaag ttaaaccagc taaagacggg ggcgataccc
     1321 caacaaagcc taaccgccga tatacattgg catcgaccag gcttaatgaa gggtttaact
     1381 ccctaccctc cacattatta actagaataa cttgctccgc cctgggaaaa cgcctttgaa
     1441 tcgcctgacg ttgctcgctg ctcatctcag ttaactcctc aagcgataat ttaatcgtcg
     1501 ctatattggc tggcaatgca ttaaaaataa gatgcaaatc ttctgttgtt ctgttattta
     1561 aatagctatc atttaaacca agatgaatta atctggaatc tttaaagact cggaatatag
     1621 ctgctaactc ctcagcggtc atcaactcca aactattctc gcctaaatca agatgagtca
     1681 cccctgtaca ctggagtgaa caaaacatcg ctgtcatttc taacatgtcc agatacccaa
     1741 tgccattacg gcataaattg agatgggtga cggaggagtc ttttagagta tcaaataggg
     1801 tctcaaaagc agtaacaacc attagatgca atccattatc acttaaatcg aggtgagtga
     1861 ttggagagtc ttttatggca ctcaacaggg atgaaaattc ggtacctgtc ctttgataaa
     1921 gatcattatt acgtaaacca agatggctta ttgaagatac tttcaatgca cggaacattt
     1981 ccgacaacac gatccccgat tttttaccaa gatcattatt ggtcaagtca agatcaatta
     2041 taggagtact actccatgcc ttaaatagtt cgattagttc agcgtctggt ctttgtccca
     2101 gccaattgca gcttaaatca agctgaatga tatctgaatc acttaaaacg gttaacaagg
     2161 tggctaactc cgtcccggtc ttttgataaa gctcattagc acttaaacta agacggctga
     2221 ttccagagtc cttaagaatc ttaaacaggg ctaccaattc agttccagtc ttttgcccaa
     2281 gattattgaa atgcaaatta agataagtga ctccaaaagc cttgagcata ctgagcagaa
     2341 ccgccaagtc agtgaatttt ttctcactca atcggttttt tgacaaattg agttggacaa
     2401 tccacaagcc ttttagctgg tccaatttga caatgatttt tttaatggat ggattaaata
     2461 gttcaacgcg catggatgca tcttatatta ttttgcaagg ctaaaaaagc ggattccagc
     2521 tgatttacaa aagctacgtc ttttcacctt aatctgtccc aatctttctc gccgtgcaat
     2581 attgtactat atcacccatt catatccata atatcttatg gactataatt ttcaaaactt
     2641 ctaagccgca taagcagtcg ttgaatcaac tagaaatttt gtataagcat atataaacaa
     2701 tcgatttcct ttcagttaat taattagaat aaaattgatg aaaaatattt caaaaagata
     2761 tcattcaaac ttatatagct cttgtctttt aacagtaggc aaaatattca ttaaaaggca
     2821 gcttattttt taacactgaa aattctggag ccataattaa aagcttgagg attgagtata
     2881 aaataatcta atatcatcaa gttactcaac aatgtcttgt tgtgagacaa cgattcaagg
     2941 agcgttatga ttgttatctt cgtccatggc tggagtgtca cgcataccaa tacttatggg
     3001 gaacttcctc aatggcttga aaatcagagc aaacagggaa agctggatat tcaagtcggc
     3061 aatatttatc ttgggcgcta catcagtttt gatgatacgg tgacggttga cgatatagca
     3121 cgcgcatttg atcaagcggt acgtgatgaa attgctgata agctacgaga tgggcagcgt
     3181 tttgcttgta tcactcactc taccggtggg cccattgtcc gcaaatggat ggatttatac
     3241 tttaagaata atcttgcaaa atgtccgtta agtcacctta tcatgctggc tcctgccaac
     3301 cacggttctg ctcttgctca gcttggcaaa tcccgcctag gccgcataaa gagttttttt
     3361 gaaggcattg aaccgggaaa gtgtgtactt gattggcttg agctcggaag cgataagagc
     3421 tggcaactta atgaaagctg gcttgattat gattgcactg ccaatggtgt ctattccttt
     3481 gtacttactg gccagaaaat tgatcgccaa ttttatgatg cagtaaactc ctacacagga
     3541 gaatctggat ccgatggggt ggtacgcgtt gctgcaacaa atatgaatta tagcttattg
     3601 aaattacatc aggaggggga taacggtgaa agtcttgttg tcgccaaaat gacacgaaca
     3661 cagccaatgg catttggagt tctacctgga ctttcacatt ccggtaaaaa tatcggtatc
     3721 atccgtagta ttactatggc caatgcagcc aatcacccca cggcgatatg gatcctgcga
     3781 tgcctgcaag taaaaagtcg tgactcctat aataaattag taaaagagtt agacaatatt
     3841 actaaagaga ctcagaaaaa tgagcataag gaatttgtga aaacacttgt ctttacgcgc
     3901 gagtatataa ccaatcgtta ctctatgatt attttccggc ttattgatga ccgcggcaat
     3961 caccttatcg attatgatct ttacctaact gccggacctc aatatagcga gcaggcgctt
     4021 cctgcaggtt tttttgtaga tcgtcagcga aatctcaata atcgaggtaa actgacttat
     4081 tttcttgact acgatattat ggaaggtgga attaataccc caaaaatgca gggtaatctt
     4141 gggtttcgtg ttaaagcata tcctgaatca agtgaccaag cccttgccta ttacaggtta
     4201 cttgattttc attcatcgct tgctgacatt cacaaaatac ttcatccgaa tgaaaccgta
     4261 atggttgaaa tcatgcttca aagacgagta gataggacag ttttccgtat cagcaacaac
     4321 cttactccgg caaaaatcag tggaaagcca actggcaaaa agatagattg atacctcttt
     4381 cgtttgatgc atttgcatca ttaatgagaa agtcatctag ccacaatttc gaaaaaaata
     4441 tcttaaatat tggtaatttt actttcttta taaatatctg aaattttttc accatcccaa
     4501 atatatctta aatgacatcc ttgtatcggt atagtgagcg cgggaggtct taatacagcc
     4561 acacataagc catttaaatc tcttacactt gggtatagca agccccattc cttcttttca
     4621 caaattttct tgccaaattc ctgactttta ctataacaag caggatcagg attaaacaag
     4681 tctttataac ttttatctgt aatatctatt aatggttttt ttatattagc cacatactct
     4741 ctcattgaga tggagcaagg tttctcattt gatgcgctat aaaaccgttc tcgatgaaag
     4801 caagtttcct ttattgctgt ttcaagtgat gaagcagcat aataaatacc aaaactgccg
     4861 tcgctaaatc ttgattcaaa tccagtatgt gtaaaagcag ccataattgg agtagatcca
     4921 ggaccaccaa tccaatcctc ttgatctatc aaatttattt taccgaactc tgcaagtatc
     4981 ctttcattgg tcaaaccttc aagtagagct atttgttcga gctcttctgc agaatctgcc
     5041 caatcgaata aagtcacggg ggggaattta gaaggtatta gtctatgcac tttatcatta
     5101 aaatctatat aggaatacat cctaataacc tctccaaaag tccagaaatt tccttacatc
     5161 tgcaagacga accatccccc cttccagcat atattctttg ggtgttaaac ctttaaacgg
     5221 aggtaagtcg tttttacgat taatccaagt tctggcttgt tccccatctt caaacaatat
     5281 acgtaaagac ttataaattc ctaaaagata ggagactcgt tctttttgat ccctgtttag
     5341 tttcgctttg tgggaagtgt acgtagagcg cggcatatcc cccatcaaaa tacgggcttc
     5401 actttctttt aaatcaaaac gttctacaag attggttaaa gacttccata ccacttcatc
     5461 tggtatgctt tgtaaagacc ttgtattagt ttgcatacta aactcctggc tgctttctac
     5521 tatattatag tctccaaaga gacgattgtc tataaaaaga catgttaagt gaattaaatg
     5581 ctcatttaat gaaaaataca ttgatctgac atgcataata tgaaataaat ttcaaaaatt
     5641 aattaacagt gcaacaagca agggaataag gtgctttaag accatagttg agcgaaaata
     5701 atttcaatat gattgataca atacagagcg aaccaaagat aaattttact tataattaca
     5761 aacaacctga tgactaccgt ttcagtcttg attcaattca cctggctaaa tttgttgcaa
     5821 aacaattgga atcctatcca gatcttggtt cattaagagt attagatctc tgtgcagggt
     5881 gtggagtaat tggaattgaa ctgtcatggt atcttcaagc aatccggcag attgatttta
     5941 ttgagatcca ggatatttat actgaatatt tttatcaaaa catagctaac gttaatcgac
     6001 ctgagctgca atttcgctgg catctcttaa attatgatga actacataaa aaaaaatggg
     6061 aagacaaatt cgatttgatt atcagtaatc caccctattt ccaaccaggt cacgggatgc
     6121 tatccccttc aaaatttaaa aatcggtgca gattttattt agatagctct tttcagagtt
     6181 atattcaagc gctgggcaat tcacttgcaa acagaggtaa agcttatttt ttattacgtc
     6241 cattaaaaca ccatggtctt gatttatttt ctgatataca aaaaatcctg caggaaacat
     6301 cagtaacagc aacaaaaata tcgcatatcc gtggtacaga tattatttta ttggaaaaac
     6361 tgaaatgaat aatagtattc tttataggac attgattttg gtctgcctta aaaaataaaa
     6421 atattttcca acacaaatta agtatattaa aattgcatgc caaagtattc ttgaatagtt
     6481 gtgccaaaat cctttgagga tcaactagag aagtagacaa tgaagtattt actgcatcag
     6541 agttagataa cgaacagacg gcattcctct acagaataaa ccattcggat aaaatggagt
     6601 tttcgatgaa agaaaatgtg tcccacctac caagagagct cagccgtatg gttttctggt
     6661 cacttacctg tttaaagaaa gccattgcag aagtctatgg aaaagaaaca tatgaaagaa
     6721 tcgagcaaat tcgactatcc atgcaagaca cgatcggaag cgaatcgttt tcacttggaa
     6781 aagctcttca tagtcttcaa atcgagcttt ccaaacttga gaaaaaacag ttatatcaaa
     6841 tagctcatgg tttttccctt atgatggagc tgattaatac ctgtgaaaat gcctaccgca
     6901 tttttcgtat caaccaaaga aaagaacaag tttattctga tatgcctcaa ggtattcatt
     6961 atgtattgac agcacaccca acagaggctc gtaccaatga atttttaaag cttttcaaag
     7021 aaattcgtga ctatcttata gaagttctta aatcccccta tctgatgaat gaatctcatc
     7081 ttgatcaaat gcttaaaatc tcacttcaaa ttaatatttc ctatcatgaa aaaccaagcg
     7141 tggaagacga ggctcaatac atctatcagt acgcactcca tccacttaac atcaaactgt
     7201 atcattcatt tttagcaaaa ggggtcccta ttcaattacg cacctgggta ggaggtgata
     7261 aggatgggca ctctggtgtt aatgaaaaaa tcatgcttaa aagcctagaa ctttctcgtt
     7321 ctttttttat tgcctacatt caggaatgct taagagaagt gctggaaatc attaaacttt
     7381 caacttcatt agataaaaat aaacccttgg cagatcagat taatcagctt attaaaaaag
     7441 cctatggtct taaaaagata caatcaaaag aacatgaaaa aatatcgata ttcaaaaatg
     7501 aattgactgc tacacagata aataccctaa aatttttaaa tttcgaacct gagcaattct
     7561 ccatcatctc cagtattttt catctgtatc ccgctttggt catgcctata gagcttagag
     7621 aggactcagc tgttgtaaaa gagagtttaa caagcataaa aaaaacaaca attactaaaa
     7681 tgcttgaaca ggtacactct ataactcatg ggactgatcc taaaaactat atacgcggtt
     7741 ttatccttag tatggttgaa tcagctgagg atataaaaaa tggaattaat ctggttaaaa
     7801 aagtttttaa aagctatgcc ctgccagttg tgcccttgtt tgaaaatcaa cttgccctca
     7861 ctaacgcaga tacaatatta aataattcaa tcactgaggc tatcagacag aagcatctca
     7921 aacagtggga tggaaactat gaagtcatgt tgggttactc cgactcgtcc aaagaaaatg
     7981 gcgtactgcc ctctcggctt atgattgcga aaagtttaaa aaacattcag tctaccctgg
     8041 aaaataaaaa ccttcggccc atcttttttc atggttcggg cggtagtatt gagcgaggag
     8101 gcggggatat caaagaacaa acagcctcct ggtcgaagga aataatgtct aattataaag
     8161 tgactgtcca gggggaaatg gttgcaagac tctttggttc aagttccata ctcaaaagcc
     8221 agattgacaa atttcttgat atctattcgc agaaaggcaa aagcaatgac aatgattatc
     8281 cggaagagtt gtccttgttt tcgacaagag ttagccagaa atataaaaca cttatccaaa
     8341 gtgactggtt ttggcaaata gtagaacaag cttcccctta tcattttctg aaagaattga
     8401 aaattggctc tcgtccaacc aaacgaaaat caggccctga tcaacgaaag cttcgcgcca
     8461 tcccatggat tctgtgctgg actcaaacac ggcttctttt tccaacctgg tggggtatag
     8521 gttccacttg gtcagagcta aatggcattg aaaaaaacag acttaaggct ctttataaag
     8581 aaaatgctct ttttgctgct tttgtaaagc aattaggtgt aaccctctcc aaggtttatc
     8641 tacccgtctg ggaacagtac ctgcttcaat tgactggctc tattgaatac ctaaggcctt
     8701 ttcaatcaga actagagtca actatccagt tcttctatca aataaccggt gagaaagatt
     8761 tctgttttca ccaaccctgg cttggggaaa gtattcaatt acgatcaaca ttgattcatc
     8821 ctttaaatct tattcagatt gaagccatga aaaggaaaga cttgcctctc ttaagaaaaa
     8881 ccgtcacagg catttcttgt ggtatgttga caacaggata atagaagtaa acaacgttat
     8941 taatgccact ctaacagagg gcaatgttat tgtagaacta atgaaatttg aacttaaaag
     9001 atatccagtg accgtcatgg cgagtattct gtaaagaata gggataagtt gagaaatata
     9061 aaatatacaa taaatgagtt tcagtatgaa gataaacaat atcccttatt atgtcgctac
     9121 ccgtgtcgta aaacgtatac agtcatggct gggattgaat actctggggg cacgagcaat
     9181 agtgactaat acagagggac acgtgttatt agtaaaacat acctaccaac ctcattggta
     9241 tttaccggga ggcggagtaa aaaaaggaga atcaaccaag gctgcagtaa tccgtgaact
     9301 gcacgaagaa gttggtcttg ttgttgcaga acaagacgtg attttattcg gcatttatca
     9361 ccacaagtat ctaggagtta atgattatcc ggtaatttat attgttaaaa actttacaag
     9421 tcatgtgact cactccgggg aaattgagca gatgggatgg ttttcccttg atgcattacc
     9481 ggaaatggtg agcccgggaa ccaagcgcag gctgggtgaa tattttgata acaatccaat
     9541 atcagaaaag tggtgattat tgcaattgtc attgttgggt gaggtgctaa gaaaatatgg
     9601 ttatgctcaa tctctaattt gtcctgtccc tcttatcacc catttgtagc ttgttaaagc
     9661 ctctaatccc ataggccctc gcgcatgaac tttttgcgta ctaattccaa tttcagcccc
     9721 taatccgaat tccccgccat ctgtaaaagc agtagaagcg ttagcataaa cagctgctgc
     9781 atccacttca gttagaaatt tatcaattgc agactcatct tcagcaataa cagcctcact
     9841 atgataagaa ctgaattgtt gaatatgatc aatagctgct tttatattgg gtactgtttt
     9901 tattgctaat ttatagtcca gaaattcatg gccaaaatct tgtggttttg ccttcattaa
     9961 aagttgttca ggataattct tatctaaaac ctgatatgca tcctggtcgg catagataat
    10021 cacattcttt tgagacaagg tctccacaag ttctggcaaa tcttttaaac gatcagcatg
    10081 aattatcaag gtatccagcg cattgcaaac acttactctt ctggttttag cattgttaat
    10141 aattttttta cctttttcca aatctccact tttgtcaaaa taggtatgca cgatgcctgc
    10201 ccctgtttca atgacaggta ttttggcatt gtctctgaca aaatttatta aattctgact
    10261 ccctcttgga atgcataaat caacaagacc ggtggcattt aataatgggg ttatcaatgc
    10321 tctttctggt ggtaataaac aaacaatatc tgtgtttata ttaaaattct ttaatgtatt
//
LOCUS       DAXLDT010000107        10335 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXLDT010000107 DAXLDT010000000
VERSION     DAXLDT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.107"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..211
                     /locus_tag="ACF2SP_002177"
     CDS             <1..211
                     /locus_tag="ACF2SP_002177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359953.1"
                     /translation="QGSNTMKKSTMAVATLLAALSTAPMVSVAYADDTSNTTTSSCTG
                     CKGCKGCNGCKGCGGCGGCGGCSGD"
     gene            282..995
                     /locus_tag="ACF2SP_002178"
     CDS             282..995
                     /locus_tag="ACF2SP_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359954.1"
                     /translation="MNSQSDFNLPILSSWYKKKFLGYASQVLEAQQKMTSSKGKNILH
                     YTLRKKRKHERMSHYPKGDRIDRSTGSQYFYHCHRENFESNEHGHFHCFLRYKHIPKR
                     IKPAPLEDWDKYIDNPMTHLVAIGMNQFGQPIRLFTVNRWVTSEIWYGAEHIPYFLKS
                     YKMTLIDDPYWQVLDQWVEGMLHLFAPQIAWLHQERDKRIQLHQLNSPNDNPYTNHEL
                     EELSEINIDLKKQIEWVIS"
     gene            1148..3490
                     /gene="purL"
                     /locus_tag="ACF2SP_002179"
     CDS             1148..3490
                     /gene="purL"
                     /locus_tag="ACF2SP_002179"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFD2359955.1"
                     /translation="MQNLTETLDFSSLNRGEIEKLLRDYNLNLSVDEALTIQNEFLKR
                     PPTISECVLWSIQGSEHCSYKSSRIHLKQFNTSGPHVILGAKEDAGIVSVAQDKNGYR
                     YGVIVSHESHNHPSQIVPYEGAATGVGGNVRDVCCMGGEVIAVADSLRFGDIKRARTH
                     WIQEGVVSGIAGYGNPLGIPNIAGDVYYDPAYNENCLVTVVTLGIVREDHIIHSYAPP
                     EAENYVFILVGKPTDNSGFGGASFASTVLEEDSQEKNKGAVQEPNAFLQRHLLKANYS
                     LFQLLREKNLIDRVGFKDLGAGGVACASIELAEAGGSYGAEIDLDKVPTGMPGLMPSV
                     ILCSETQERFMWVVPPDLVDTILKHYNETFALPQVSEGACAAVIGKIRTDGLYVVNYK
                     GRELVRAKVPDVTKGIVYNRPHASSQRQNTEPFFLPPDDYNQILLQLLAHENIASKEP
                     IFEMYDKQVQGRTLIQAGWADAGVLQPFNESKYPEEIRKTGIALSLDQNPRYNKIDAY
                     WGAVNAVVESVRNITAVGATPVAITDCLCFGNPEKPEQMREFVDSVRGISDACAAVHL
                     KDYPQSTLPVIAGNVSLYNESVKGAIPPSPMISCLGTLPDIDFAITFDFKKSDSLLIL
                     IGERKDECGGSVYYQLHNQLGSNVPKPDLSLFNREIHAVSSAIQHGLVNAAHDVSEGG
                     VAVALAEMSFKNSLGVAVQINGELSADKLLFGETGGFILEIDKQHKAAFDKLVTQYQV
                     PYMVIGHTTEQPVLQMNSVINLPVEEARQAWENGLRERLL"
     gene            3490..4533
                     /gene="purM"
                     /locus_tag="ACF2SP_002180"
     CDS             3490..4533
                     /gene="purM"
                     /locus_tag="ACF2SP_002180"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFD2359956.1"
                     /translation="MSTINYKSAGVDIEAGNEAVARIKRNVEKTFSPQVLTGIGSFGA
                     MYDLKPFMEKFKHPVMVQSIDGVGTKTIVARMMQKFDTIGIDLVSATTNDIIVLGARP
                     LTLLDYIATDRLKPEYIEEIIIGMTQACLENNISLVGGETAEMPDTYLKGEHDLVGII
                     TGVVEKDEAILGKDITPGDSVLAFPSSGLHTNGYSLARKLLFELGGYTVESQLDELDA
                     TVGEALLVPHINYTNPVLHLLNNKIPIKGMAHITGGGVLENIPRVLPNHCSVEINKNS
                     CPVLPIFNVLKALGKLDDHEMYRTFNMGIGLVMIASPGVVSEIHTALKEFPNFPVYEV
                     GKVVSGKPEVRLL"
     gene            4533..5792
                     /gene="purQ"
                     /locus_tag="ACF2SP_002181"
     CDS             4533..5792
                     /gene="purQ"
                     /locus_tag="ACF2SP_002181"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HFD2359957.1"
                     /translation="MKIAVVQFPGSNCERETMLAVQRAGMTPVEFLWNDSSQKLREMD
                     GYIIIGGFSYEDRSRAGIIAALDPVMKEIKLQSELGKPVLGICNGAQILVETGLVPGL
                     KNYQIGMALTENKRIQDGKILGTGFYNSWIHMRTGDRVRQNAFTRYLSKDSVLSIPAA
                     HAEGRFVMPVKLLQEIEEEGLNAFQYCDAQGNRDDNFPVNPNGSLRNIAAIINKAGNV
                     MAVMPHPERTFAGDPIFHSMKEYIKEKKTFKQQTLSYQPEPVKIIPYDKKDNAHELIV
                     KLIITDNAALTVQNTLRQLGIPVTVNRMMYWHVDTNFPETIEKIKQTGVLYNDRKEYL
                     VTHEHIFSSAAKAFLVKSKDDMIGQQKLQMLRDHFSIDGVDAIHHGVLWIFSSENVKI
                     QELAESILATNIIFNPNAHICYEYQCP"
     gene            5773..6753
                     /locus_tag="ACF2SP_002182"
     CDS             5773..6753
                     /locus_tag="ACF2SP_002182"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFD2359958.1"
                     /translation="MNTNVPSQEDLLAALPFCLTETSLPFGKKYKGKVRDTYDLGDQL
                     ILVTTDRQSAFDRCLAAVPYKGQVLNLTSVWWFKNTQSIVPNHLIAVPDPNVAIAKKC
                     KIFPIEFVVRGYISGSTSTSLWTQYQKGVREYCGITFPDGLRKNQKLESPVITPTTKE
                     TLHDRPISPHEIVAEGWMTQEDWDETSSYALKLFQHGMEVAQQHGLILVDTKYEFGRD
                     AEGRIVLVDEIHTPDSSRYWLFNGYQERFDAGKEPENIDKEFLRLWFVDHCDPYKDEV
                     LPQAPQELIVTLASRYIQLYEMITGESFVYDSNPGPVNDRILHNIQRWLG"
     gene            6776..8275
                     /gene="purF"
                     /locus_tag="ACF2SP_002183"
     CDS             6776..8275
                     /gene="purF"
                     /locus_tag="ACF2SP_002183"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFD2359959.1"
                     /translation="MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFK
                     HGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGN
                     LVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
                     EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHEDGSKDYIFASETTPFYA
                     LGFEPKGDLLPGEVAYVNQQGQLYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRA
                     RLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGIRYSEGLYKNPFIGR
                     TFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEI
                     YFVSTCPPIKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSR
                     RGQYDMSKPCMACMDGKYICGQITEEKIKLLEKKRLTENDQETEIGEMS"
     gene            8272..9591
                     /gene="purD"
                     /locus_tag="ACF2SP_002184"
     CDS             8272..9591
                     /gene="purD"
                     /locus_tag="ACF2SP_002184"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFD2359960.1"
                     /translation="MINILIVGSGAREHAIATALSKSENKPKIYCFGTTRNPGIQRLT
                     SDYWVGDITDPEQVNQQAKEWEINLAIIGPEAPLEHAVADALWDCGIPVIGPRKNLAK
                     IETSKAFARELMEKYGIPGLPKYKVFTSLEGIREFLSELGENNYVVKANGLMAGKGVK
                     VGGEHLLSFEEALNYCKEIFTHNQSLVIEEKLIGQEFSFMCFSDGISLVPMPIVQDHK
                     RAYEHDEGPNTGGMGSYSAADHSLPFLSPEDVRAAFEINQSVINALMTEMSDKYIGIL
                     YGGFMATAHGVYVIEFNARFGDPEALNVLSLLESDFVKLCVAMANGNLQKESARFLNQ
                     ATVCKYAVPDGYPDNPLRHVEIDISAVEPKDTLYLAAVNFKDGKLYATGSRTAAYVGI
                     ANTISEAEEIAEQQIKNIKGPLFHRKDIGTSPLINTRVEVMRRLRAL"
     gene            9588..10166
                     /gene="purN"
                     /locus_tag="ACF2SP_002185"
     CDS             9588..10166
                     /gene="purN"
                     /locus_tag="ACF2SP_002185"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFD2359961.1"
                     /translation="MIRLGILGSTRGTNMLALVDAINEGTLKAKIELVISNKPDAIIL
                     ERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNN
                     QVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
                     EGDTAQTLKARVQQLEGMALVAAINLIASKGF"
BASE COUNT         3162 a         1757 c         2316 g         3100 t
ORIGIN      
        1 tcaaggaagt aatactatga aaaaatcaac aatggcagta gccactctgt tagctgcttt
       61 atcaacagca cctatggttt ccgtagctta tgctgatgat acttcaaata caacaacttc
      121 tagctgtact ggttgtaaag gatgcaaggg ctgcaacggt tgcaaaggct gtggcggttg
      181 tggtggctgc ggtggttgca gtggtgatta ataagtaata ttttctataa agaggtagtg
      241 ttaactacct ctttttatta cggtttattt tttaagtaat tatgaatagt caatcagatt
      301 ttaacttacc catattgtcc agctggtata aaaagaaatt tcttggttat gcttcacaag
      361 ttcttgaagc ccaacaaaaa atgacatcca gtaaaggtaa aaatattctg cactacacgc
      421 ttagaaaaaa gcgaaagcat gaacgcatga gtcactatcc taagggagac aggattgatc
      481 gtagtactgg ctcacaatat ttttatcatt gccatagaga aaatttcgaa agtaatgaac
      541 atggtcattt tcattgtttt ttgcgatata aacatatacc gaagcgtatt aaaccagctc
      601 cattagagga ttgggataaa tacatagata atcccatgac tcaccttgtt gcgataggta
      661 tgaatcagtt tgggcaaccg atacgattat ttacagtcaa tcgttgggta acatcagaaa
      721 tttggtatgg tgctgagcat attccatatt tcctgaaatc ttacaagatg acattaatag
      781 acgatcctta ttggcaagta ttagatcagt gggtggaagg catgctccat ttgtttgctc
      841 cccaaattgc ctggcttcat caggagcgag ataaacgcat tcagcttcac caactgaata
      901 gcccaaatga taacccgtac actaaccatg agctggaaga gctttctgag ataaatattg
      961 atttgaaaaa gcagattgag tgggtaataa gttaaattcc aaaattagaa ccacaactta
     1021 atttatctta attattggtt tttcattgct tataccccgt gtaaaaatat acaatatcta
     1081 ctgctttggg gagagattag tcctttactg atcaatcaga gattaattct atctggtata
     1141 gagattcatg caaaatctta ctgaaaccct tgatttttct tctttgaatc gtggagaaat
     1201 tgaaaaatta ctgcgcgatt ataatttaaa tctttctgta gatgaggcgt tgactataca
     1261 gaatgaattc ctcaaaagac ccccgactat ttcagagtgt gtactttggt ctattcaagg
     1321 atcagaacat tgttcttata aaagcagccg tattcattta aagcaattca atacctcagg
     1381 ccctcacgtg atactgggtg ccaaggagga tgcaggtata gtatctgtag cccaagataa
     1441 aaatggttat agatatggcg ttattgtcag tcatgaatca cataatcacc catctcaaat
     1501 tgttccctat gaaggcgcag ctaccggagt tggtggtaat gtccgtgatg tctgttgtat
     1561 gggtggggaa gttatagcag tagcagacag tttgcgtttc ggagatatca aacgtgcacg
     1621 tactcactgg atacaggaag gggtggtttc aggtattgcc gggtatggca atcctttggg
     1681 aataccgaat atagctggtg atgtgtacta tgatccggcc tacaatgaaa attgtttggt
     1741 tactgtagtc actctgggga tagtaagaga ggatcatatt atacattctt atgctccgcc
     1801 cgaagctgag aactatgttt ttattttagt aggtaagcca actgataata gtgggtttgg
     1861 cggcgcgagc tttgcctcaa cagtattgga agaagattcg caagaaaaga ataagggagc
     1921 tgttcaagag cccaatgctt ttttacagag acatctcctt aaagctaatt attcattatt
     1981 tcaattacta agagaaaaga atttaattga cagggtaggc tttaaagatt taggagcagg
     2041 tggcgtggcc tgcgcaagta tcgagttggc tgaagccggc ggttcttatg gcgccgaaat
     2101 tgatctggac aaggtaccca caggaatgcc aggattaatg ccttcagtca ttctatgctc
     2161 tgaaactcag gagcgtttta tgtgggttgt tcctcctgat ttagtagata ctatcctgaa
     2221 acactataat gaaaccttcg cgttaccaca ggtttctgaa ggggcatgcg ctgcagttat
     2281 cggcaaaatt cgtaccgatg gattatacgt ggtgaattac aaagggcgag aacttgtgag
     2341 agctaaagtc cctgatgtca cgaaaggcat tgtttataac aggccacatg cctcttcgca
     2401 gaggcaaaat acagaaccat tttttctccc accagatgat tataatcaaa ttttgttgca
     2461 gttattggcc catgagaata tagcgagcaa ggaacctatt tttgaaatgt atgacaagca
     2521 agtgcagggt cgcaccctca ttcaagcagg atgggctgat gctggagtat tacaaccatt
     2581 taatgaatcg aagtatcctg aggaaattcg taaaacaggt attgccttgt ctttagatca
     2641 aaatcctcgc tataacaaaa ttgatgccta ttggggagct gttaacgcgg ttgttgaatc
     2701 ggtacggaat atcactgcag ttggagctac tccagtcgca ataacagatt gcttgtgttt
     2761 tggtaatcca gaaaagccag aacaaatgcg cgaatttgtt gattctgtgc gaggaattag
     2821 tgatgcctgt gccgctgttc atctgaaaga ttatccccaa tcaacgttgc ctgtcattgc
     2881 cgggaatgtc tcattgtata atgaatcagt gaaaggggct attcctccca gccccatgat
     2941 tagttgcctg ggaacgcttc cagatattga ttttgctatt acctttgatt ttaagaaaag
     3001 tgatagccta ttgatattga ttggagagcg taaagacgaa tgtggtggta gcgtttatta
     3061 tcagctccat aatcaattgg gttctaatgt tccaaaaccg gatttaagct tgtttaatag
     3121 agaaatccat gctgtttcat cggcaattca gcatgggttg gttaacgctg ctcatgatgt
     3181 ctcagaggga ggggttgctg ttgctcttgc tgagatgagt tttaagaact ccctgggagt
     3241 tgctgtacag attaatggag agttatctgc ggacaaactt ttatttggag agacaggcgg
     3301 ttttattctt gaaatcgata aacaacataa agccgctttt gacaaactgg ttactcaata
     3361 tcaagtccct tacatggtta tcggacatac tacagagcag ccagttttac agatgaattc
     3421 ggttatcaac ttgcctgtcg aagaagcgag gcaggcttgg gaaaatggct taagagagag
     3481 gctattataa tgtctactat caattacaaa tcagcgggtg tcgatatcga ggcgggaaat
     3541 gaagctgtcg cacgcattaa acgtaatgtt gaaaaaacat tttcgcctca agtcctgaca
     3601 ggaatcggta gttttggcgc aatgtatgat ttgaagcctt ttatggaaaa attcaaacac
     3661 cccgtgatgg ttcaaagcat tgatggcgta ggtacgaaga caatagttgc tcgaatgatg
     3721 caaaaatttg atacaatcgg aattgatttg gtcagtgcga ccaccaacga tattattgtg
     3781 ttgggtgcaa gaccattaac acttttggac tatatagcaa ctgatcgatt gaaacctgaa
     3841 tatattgaag aaattattat tggcatgacg caagcttgcc tcgaaaataa tatttccctg
     3901 gtcggtggtg agacggctga aatgcctgat acctatctga agggagagca tgatttggtt
     3961 ggtattatca ctggtgtagt ggaaaaggat gaagccatac taggaaaaga tataactcca
     4021 ggcgattcag tcttggcatt tccttccagt ggcttacata ccaatggata ctcactcgcc
     4081 agaaaacttt tgtttgaact aggtggatat actgtagaat cccaacttga cgagttggat
     4141 gctacagttg gtgaggcttt actggtacct catatcaact atacaaatcc tgttctacac
     4201 ctgttgaata acaaaatccc tatcaaggga atggctcata ttactggcgg aggtgtactg
     4261 gaaaacattc cgcgagtatt acctaatcat tgttccgttg aaatcaataa gaattcatgt
     4321 ccggtgttgc cgatctttaa tgtgctaaaa gcattgggga aactggatga tcatgaaatg
     4381 tatcgtactt ttaatatggg aataggatta gtaatgatag cttcccctgg tgttgtttct
     4441 gaaattcata cagcgctaaa agaattccct aatttccctg tttatgaagt aggaaaagtc
     4501 gtatcaggaa aaccagaagt gagattattg tgatgaagat tgcagttgtt cagtttccag
     4561 gctccaattg cgaacgtgag acaatgcttg cggtgcaacg agcaggtatg actccagttg
     4621 agtttctgtg gaatgattca tcacaaaaac tgcgagaaat ggatggttat attattattg
     4681 gtggtttttc atatgaggat agatcaagag caggaattat tgcagccctt gatccggtaa
     4741 tgaaagaaat aaaattgcag agtgaattgg gaaaacctgt tcttggtatt tgcaatggag
     4801 cacagatcct tgttgaaacg ggcctcgttc ctggtttaaa aaactatcaa attggtatgg
     4861 ctctgactga gaataagcga attcaggatg gaaaaatttt aggaacagga ttttataaca
     4921 gctggattca tatgcgaacc ggtgaccgag tgaggcaaaa tgcattcacc cgctatttat
     4981 caaaggattc tgtattatca attcctgctg cccatgctga agggcgtttt gtgatgcctg
     5041 ttaaattatt gcaagaaatt gaagaagaag gtttaaatgc gtttcaatat tgtgatgcgc
     5101 agggaaacag agatgataat ttccccgtta atcctaatgg ttctctacgg aatatagccg
     5161 ctattattaa taaagcgggt aatgtcatgg ccgtaatgcc tcaccctgaa cgtacatttg
     5221 cgggagatcc tatattccat tcaatgaagg aatatattaa ggagaaaaaa acattcaagc
     5281 agcaaacatt atcttatcaa ccggagccag ttaaaataat tccttatgac aaaaaagaca
     5341 atgcccatga gttgattgtt aaattgatta ttaccgataa tgctgcttta acagtacaaa
     5401 atacgctaag acagttaggt ataccagtca cagttaatcg tatgatgtat tggcatgtcg
     5461 atacaaattt tcctgaaaca atcgaaaaaa taaagcagac aggggtttta tataatgatc
     5521 ggaaagagta tttggtaacg catgagcata tattttcatc agcagccaag gcttttttgg
     5581 ttaaatcaaa agacgatatg atcgggcaac agaagctgca aatgcttcgg gatcatttct
     5641 caattgatgg cgttgatgcc atacaccatg gtgttttatg gatatttagc agtgaaaatg
     5701 ttaaaataca agaattggca gaatctattt tagctactaa tattatcttt aaccctaatg
     5761 ctcatatctg ctatgaatac caatgtccct agtcaagagg atcttcttgc tgcgttgcct
     5821 ttttgcttaa ccgaaacctc tttacctttc ggcaaaaagt ataaggggaa agtaagggat
     5881 acctatgatc ttggcgatca attgattttg gtaactacag atagacaaag tgcctttgat
     5941 cgatgccttg ctgctgtacc ttataaagga caagtattga atttgacttc agtatggtgg
     6001 tttaaaaaca ctcaatcgat tgtacctaac catttaattg ccgtacctga tcccaatgtg
     6061 gcgatagcca aaaaatgcaa aatattccct attgaatttg ttgttcgtgg gtatatttcg
     6121 gggagtacaa gtacgtcttt atggacgcaa taccaaaaag gagttcgtga gtactgtggt
     6181 ataacgtttc ctgatggatt aagaaaaaat caaaaacttg agagcccagt gataaccccc
     6241 actactaaag aaacgctcca tgacaggccg attagcccac acgaaatcgt cgcagaaggc
     6301 tggatgaccc aagaagattg ggatgaaacc agcagttatg ctttaaagtt atttcaacat
     6361 ggaatggaag tagcacagca acatggtttg attttggtgg ataccaaata tgaatttggc
     6421 cgtgatgcgg aaggaagaat tgtattagtg gatgaaattc atactccaga ttccagtcgt
     6481 tattggttat ttaacggtta tcaagagcga tttgatgcgg gtaaggagcc ggagaatata
     6541 gataaagaat ttttgcgctt gtggtttgtt gatcactgcg atccttataa ggatgaagta
     6601 ttacctcaag caccacagga gttaattgtt actttagcat caagatatat ccaattatat
     6661 gaaatgatta caggtgaatc gtttgtgtat gacagtaatc ctgggccagt aaatgatagg
     6721 attttgcata atatacaacg ttggttaggt taatttttag ccgggggaaa ggcttatgtg
     6781 cggcattgta ggaatttaca gtcatgaacc cgttgctgct gagctttatg atagcttgat
     6841 tcatttacag caccgggggc aagatgcctc aggtatttta accggagctg atcgttttta
     6901 cttcaaacat ggacttggac tggtcagaga gtctattttc tctgaagata ttcttaaact
     6961 gaaggggaat atcggcattg ctcatgtgcg ttatcccacg gccggaggtt atagtgaggc
     7021 ggatgttcag cctctttgga tagggagccc tcgcgggata gctctggctc ataatggaaa
     7081 tttagtgaat tatggagagt tggttgatga tttatgcgga aaacagcata ggcatttaaa
     7141 ctctactctt gattcagagg cacttttatt acttctggca gactatttag ccaagggatc
     7201 ttattccgat catgatgaag agcaattctt tcaattatta tgtatggcag ttaatgaaat
     7261 acatcaacga gttcaaggtt cctattctgt tgtgagttta gtaattggca aaggtttggt
     7321 tgcatttagg gatccgcatg gtattcgtcc tttagtttgg ggtgagcgag tccatgaaga
     7381 tggtagtaaa gattatattt ttgcttcaga aaccacgcct ttttatgctt taggttttga
     7441 gcccaagggt gatttgctgc caggtgaagt tgcttacgtg aatcagcaag gacaattgta
     7501 cagaaaagtg ctttcatcaa aaaaatttac accttgtata tttgaatacg tttacttcgc
     7561 cagaccagat gccacattag acgatgtgag cgtttatcga gccagattac gtatggggca
     7621 aaatttagct aaaagctgga aaaagagatt tccagataca ctacctgatg tggtgattcc
     7681 agccccttca acagcaaata ctgccgcatt agcttttgca aatgagttgg gaatacgtta
     7741 ttccgaaggt ttatataaaa atccttttat tggacgtact tttatcatgc ctaatcagga
     7801 gtcgcgatca agacaggttc gctataaatt aactcctcag aaaacggaaa ttaataaaaa
     7861 aaaggtatta atcgttgatg atagtatcgt aaggggaact acctcacgcg aaatagtcag
     7921 aatgatacga gaatatggag catcagaaat atattttgta tcaacctgtc caccaattaa
     7981 aaacccttgt ttctacggga ttgatatccc atcgcgtaaa aaattgatag cagcccataa
     8041 aacggaagag gaaatccgtc aatttctagg ggtggataaa ttattatacc aaactcagga
     8101 aaaccttata gaggctgtgt ctcgcagggg gcaatatgac atgagcaagc cttgtatggc
     8161 ttgtatggat ggcaaataca tttgtggaca gattactgaa gaaaaaatta aattgttgga
     8221 gaaaaaaaga ttaaccgaga atgaccaaga aacagagata ggagagatgt cttgatcaat
     8281 atccttattg ttggttcagg tgcgcgtgag catgcaatag ccacagcatt gagtaaatca
     8341 gagaataaac caaagattta ttgctttgga acaactcgta atcctggtat ccaaaggctc
     8401 acctcagatt attgggtagg tgatattaca gatcctgagc aagttaacca gcaagcaaaa
     8461 gaatgggaaa ttaatttggc cattattggt ccagaagcgc ctttggaaca tgctgttgct
     8521 gatgctttgt gggattgtgg aattcctgtt ataggcccca ggaaaaattt ggctaaaatt
     8581 gaaaccagta aagcgttcgc cagagagttg atggaaaaat acggtattcc aggtttgcct
     8641 aagtataaag tatttaccag tttagaagga ataagagagt ttttatcaga gttaggtgaa
     8701 aataattacg ttgttaaggc caatggttta atggcgggca agggtgtaaa agtaggtggg
     8761 gaacatctgt tgtcatttga agaggcttta aactattgta aagaaatttt cactcataat
     8821 cagtctttag tcattgaaga aaaattaata ggtcaagaat tttcatttat gtgtttttct
     8881 gatggcatct cattagttcc aatgcctatt gtgcaagatc ataaacgagc atatgagcat
     8941 gatgagggtc ctaatacagg tggtatgggg agttattcag cggcagatca cagcttgcca
     9001 tttttgtctc cagaggatgt tcgtgctgct tttgaaatta atcaatcagt aatcaatgcg
     9061 cttatgacgg aaatgtctga taaatacata ggaatacttt atggcggatt tatggccacc
     9121 gcacatgggg tgtatgtgat tgagtttaat gctcgctttg gagatcctga ggctcttaat
     9181 gtgcttagct tgttagagtc tgattttgtt aaattatgtg ttgcgatggc taacggcaat
     9241 ttacaaaaag aatccgctcg ctttttgaat caagccacag tttgtaaata cgctgtccct
     9301 gatggttatc ctgataatcc attgcgccat gttgaaattg atatttctgc tgtcgaacct
     9361 aaagatactc tatatttggc tgcagtgaat tttaaggatg gcaaactcta tgccacaggt
     9421 tcgaggacag ccgcttatgt cggtatagcg aatactatca gtgaggcaga agaaattgct
     9481 gaacaacaaa taaaaaatat aaaaggccct ttatttcaca gaaaagatat tggcacatcg
     9541 cctttaatta atacacgcgt tgaagtaatg cgtaggttgc gtgccttatg attcgtttag
     9601 gaatattagg atcgactcgt ggaaccaata tgttggctct tgttgatgca atcaatgaag
     9661 gaactcttaa ggcaaagata gagcttgtta ttagtaataa acccgatgct attattctgg
     9721 aaagagccaa atcgttgggc ttgaatgcac aatttgtcaa ccctgaggga ttaaacagaa
     9781 ttgattttga taaaaaagta agtgacatac tgatcaacca tcagattgat ttgattgttt
     9841 tgattgggta catgagaatt ttgtcggcgg atttcgtaaa taaatggaat aatcaggtaa
     9901 ttaatgtaca tccctcttta ttgccagcat ttgcagggaa aatggatatg gatgtacatc
     9961 aagctgttct tgattccggc ctaaaggaaa caggttgtac tatccatttt gtcactgaag
    10021 aggtggatgc tggtcctgtt attttgcaaa aaaaatgccc tgtcttagaa ggagatactg
    10081 ctcaaacgtt aaaagcaagg gttcagcagt tggaaggaat ggctttggta gctgcaatta
    10141 acctgattgc atcgaaaggt ttctaatgca agattgtatt tccagtcaaa gcagtatggt
    10201 cttcatttcc atgagaatag tttttttctt tttcaattgt aaaaaaagtg tttaacgaag
    10261 tttctttaaa ctcaaataaa tggattccat tcagtttaat actggtgtct gtatggactc
    10321 gattatttac agcag
//
LOCUS       DAXLDT010000108        10199 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXLDT010000108 DAXLDT010000000
VERSION     DAXLDT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.108"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            163..570
                     /locus_tag="ACF2SP_002186"
     CDS             163..570
                     /locus_tag="ACF2SP_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFD2359962.1"
                     /translation="MRPCYFVCILALFMFAMPGYSENHHPQEFLKSIAGTKDEGEKIF
                     NHFCVNCHGTKPMISLGAPEIGNERAWGERLKQGMEVLFKHTDEGFNAMPPRGGCFEC
                     TDKQLILAIIAMVPKQAQKGLLNNLLGHKEYKQ"
     gene            647..1204
                     /locus_tag="ACF2SP_002187"
     CDS             647..1204
                     /locus_tag="ACF2SP_002187"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFD2359963.1"
                     /translation="MKKQILLVPVCVLAASCASMDRSTAIVDDAGYTHYTMSMESDRK
                     GSSYFPEKRQATGKKVFIFDPKATAWAAYDAQGNRVKTGSASGGKDFCEDVGRPCRTV
                     TGSFRVYSKKGEECTSSIYPIETGGGAKMPYCMHFHGGYSIHAAYEVPNYNASHGCIR
                     VLPSAAKWLNQNFIDVGTSVIVKPY"
     gene            1491..2261
                     /locus_tag="ACF2SP_002188"
     CDS             1491..2261
                     /locus_tag="ACF2SP_002188"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HFD2359964.1"
                     /translation="MILCIDVGNSHIYGGVFDGDEIKLRFRHTSKVSTSDELGIFLKS
                     VLRENNCSPETIRKIAICSVVPQVDYSLRSACVKYFSIDPFLLQAGVKTGLNIKYRNP
                     VEVGADRIANAIAATHSFPNQNIIVIDFGTATTFCAISHKKAYLGGAILPGLRLSADA
                     LSKNTAKLPSVEIIKTESVVGRSTIESIQSGVYYGVLGACKELIQRIHHEAFNGDQIL
                     ILATGGFASLFDKQGLYDHLVPDLVLQGIRLAAMMNTA"
     gene            2879..3337
                     /gene="mraZ"
                     /locus_tag="ACF2SP_002189"
     CDS             2879..3337
                     /gene="mraZ"
                     /locus_tag="ACF2SP_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFD2359965.1"
                     /translation="MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETC
                     LLLYTAAQWQIIEDNLQKLPSFNAAARRIQRLLIGHATDVEVDANGRVLLPTVLRNYA
                     KLEKDVVMIGQGNKFEVWNKELWESKREQWLAEEASMTDGLPEEMKTFSL"
     gene            3349..4275
                     /gene="rsmH"
                     /locus_tag="ACF2SP_002190"
     CDS             3349..4275
                     /gene="rsmH"
                     /locus_tag="ACF2SP_002190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFD2359966.1"
                     /translation="MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSD
                     KGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLG
                     VSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
                     GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDL
                     SNCLEQCLDVLGPGGRLAVISFHSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNF
                     KKVGKAVKPQSSEIKENVRSRSAVLRIGEKLA"
     gene            4272..4610
                     /gene="ftsL"
                     /locus_tag="ACF2SP_002191"
     CDS             4272..4610
                     /gene="ftsL"
                     /locus_tag="ACF2SP_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFD2359967.1"
                     /translation="MNAAAKVINQGNLFNGQLADMQMSKSLYMLIVLLAAVLVSAFAV
                     IYSTNSYRLTLSQVQQEEQQTHFLQLQWGQLLLEQASLATPARVEELAREKLGMTLPT
                     SANTYLLHPQ"
     gene            4991..6652
                     /locus_tag="ACF2SP_002192"
     CDS             4991..6652
                     /locus_tag="ACF2SP_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFD2359968.1"
                     /translation="MKKTHHFARLVTVAVFFSLILAILIWRMVDLTVLHRQFLQGQGD
                     ARSIRVIDIPAYRGMITDRNGTPLAVSTPVESVWVNPKEFSPDEEQFMQLAKYLNLAP
                     KELSRKIVRAENREFVYLQRQLPPPLSKKIEALKIPGVNFQKEFKRYYPDSDSISPLI
                     GFTNVDDQGLEGIELAYQDWLMGVVGKKRVIKDRLGRVVEDLGVLKEPRPGHELTLSI
                     DRRLQYLAYSELGKTVEEFSAKSGSVVVIDAENGEILAVANVPSYNPNARGHYDKETY
                     RNRAFTDTFEPGSVIKSFSIASALETGLFTPDTIIDTNPGWMTVHGRTVRDIHNYGVL
                     DVTGILQHSSNVGVTKMVLSTPAEQLIGFLQKCGFGQRSELAYPGESEGGIVKAKDAN
                     PFVLATLGFGYGLSVTALQLAKANLIFANKGRLIPVTLIHNDTPSNGIQVMQPKTAEN
                     VLLMMEAVMNKGTGKGARVPGYRVAGKTGTARVAGKNGYKDRKYTASFIGIAPVSKPK
                     FVVAVIIHEPSRKGYYAASVAGPLFAKVMSGALRLFNIPTDEPVS"
     gene            6673..8124
                     /locus_tag="ACF2SP_002193"
     CDS             6673..8124
                     /locus_tag="ACF2SP_002193"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFD2359969.1"
                     /translation="MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAAS
                     DGRLYIEKAVASGAVAVVYEPNNLPQGITLPTGIPCIAIPQLAEKLGEIAKRFFNNPS
                     QFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
                     LQRLMHQYKEGGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYG
                     AAKSRLFERAELQWAIINQDDDFQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDIT
                     GTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEKVVQVMAELKAAPGR
                     MEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAA
                     SMYADNIVITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDI
                     ILIAGKGHESYQQIGNTKHVFSDQEVVRRLMNN"
     gene            complement(8169..9221)
                     /locus_tag="ACF2SP_002194"
     CDS             complement(8169..9221)
                     /locus_tag="ACF2SP_002194"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HFD2359970.1"
                     /translation="MNILADALLPGLDSAFPPPFTVTLYHKADEIPELLHYKDVLLCR
                     STLKINGDLLKNHQIKFVATATSGTDHIDFPFLESQNISIIDAKGCNAISVADYVVAC
                     LAYLDKQQLIQGKTAGIIGLGQVGTKVYERLNAAEFQLCLYDPPKATRDTSFQSCSLE
                     DLFECDLLCVHAELHSDAPYPSLNLINRDFLKELKPGCIIINASRGGIVNEEALLHLG
                     SAILYCTDVYNNEPHIDSRIVSKATLCTPHIAGHSLEAKFAAVAIVSRKLHQMLGLPY
                     PQFATPEKPYRLNENSDWRELALSIYNPIHETLELKHAGNLSSAFLTLRKNHHHRHDF
                     TTYFDSDSIKKYPLLG"
     gene            complement(9218..9877)
                     /locus_tag="ACF2SP_002195"
     CDS             complement(9218..9877)
                     /locus_tag="ACF2SP_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFD2359971.1"
                     /translation="MPEFTLIQQICIWALPVLLAITFHELAHGYVAYRCGDNTAKMFG
                     RLTLNPIKHIDPIGTVLLPLLIGVITNFNFVIGYAKPVPVNQSQLRKPRRDMILVTLA
                     GPLANLIMAFLWAGCFKIATLLSPQSSMTALFLLVTARAGMMINLILAFLNLIPIPPL
                     DGSKVVINLLPARQAIAYAKLEPFGFLILIVLIFTNVLGYILTPLIYGSLYILSAIFN
                     L"
BASE COUNT         3199 a         1728 c         2225 g         3047 t
ORIGIN      
        1 cgagagactg acttgattct gaaaataaac tggtgcttgc aaaaaataag aaaaagctta
       61 gtatgcactt tttcacgaca taaactccct tagtctattg attttttata ttgaaacgca
      121 ttgtgtccca agcaagtatt gcgccaattt taggagtata tcatgagacc ttgttatttt
      181 gtttgtatat tggctttatt tatgtttgcg atgccagggt actctgagaa tcatcatcct
      241 caagagtttc taaagtcaat tgctggcaca aaagatgaag gggaaaaaat ttttaatcat
      301 ttttgtgtta attgccatgg aacgaaacca atgatttctt taggagcccc tgaaattgga
      361 aatgaaagag catggggaga acgattgaaa caaggaatgg aagtgctttt taaacacacc
      421 gatgaagggt ttaatgccat gcctcccaga ggaggatgct ttgaatgcac tgataaacag
      481 ctgattctag ctatcattgc gatggttccc aaacaggctc aaaaaggact attaaacaat
      541 cttttaggtc ataaagaata caaacagtaa aaaattttta taaaaaagcc taaacttatt
      601 caaactgctc ccgatatagt taaaaagaga aagagggaga tagaaagtga aaaaacaaat
      661 actgttagtt ccagtttgtg tcttggctgc ctcttgtgca tccatggata gatcaacggc
      721 catagtcgat gatgcgggtt atacccatta cactatgagt atggagtccg atagaaaggg
      781 gtctagttac tttcctgaga aaagacaggc cacaggaaaa aaagtattta tttttgatcc
      841 taaagctact gcctgggcag cttacgatgc tcagggtaac cgagttaaaa ccggtagtgc
      901 ttcaggggga aaagattttt gtgaggacgt tggcagaccc tgtcggactg taacaggcag
      961 ttttagagtt tattccaaga agggcgagga atgtacctcc agtatctatc caatagaaac
     1021 tggcggtgga gcaaaaatgc cttattgcat gcattttcat gggggttatt caatacatgc
     1081 tgcctatgaa gttcccaact ataacgcaag tcacggctgc atccgggttt tgccaagcgc
     1141 tgcaaaatgg ttaaatcaaa actttattga tgtaggaact tcggtaatcg ttaaacctta
     1201 ttaattaagt aaagattctc cactactccc cgctaattgc ggggtttttt tattgggtga
     1261 ttttattatc ttgttctggc aaaaagacaa tggttttatg acaattgttt tatgtaagaa
     1321 gtccagctaa agtttgggtt gggatgaaag cggattcaac aataatttga attaataata
     1381 tatagaatgc tctcaataca agtctatttc tttccgggat tgctgaatta ttgtcttaga
     1441 cctataatca caaatcacca attatgccat ttaaaaagta gatcttaata atgattctgt
     1501 gtattgatgt aggaaactct catatatatg gtggagtatt tgatggagat gagattaagc
     1561 tccggtttcg tcatacttcc aaggtaagta cttcggatga attgggtatc tttcttaaaa
     1621 gtgttttacg tgaaaacaat tgctctcctg agacaattag aaaaattgct atttgttcag
     1681 tagtacctca agtggattac tcattgcggt ctgcctgtgt gaagtatttt tcaattgacc
     1741 cgtttttatt acaggcaggg gttaagactg gattgaatat caaatataga aatccggttg
     1801 aggttggagc tgaccgaatt gcgaatgcta ttgccgccac tcatagtttc cctaatcaaa
     1861 acattattgt tattgatttt ggtacagcaa ctactttttg tgccatttct cataaaaaag
     1921 cttatttagg tggtgctatt ttgccaggcc ttagattatc tgctgatgct ttgtcaaaaa
     1981 atactgcaaa gttaccttct gttgaaatta tcaagaccga gagtgtagtt gggcgttcta
     2041 ccatagaaag cattcagtct ggagtctact atggtgtcct tggtgcatgt aaagaattaa
     2101 ttcaaagaat acatcatgaa gcgttcaatg gcgatcaaat actaattttg gcaacagggg
     2161 gatttgcgtc tttatttgat aaacaaggtc tttatgacca tcttgtccca gatttggttc
     2221 tccaggggat aagattggct gctatgatga atactgcctg agcagtaaaa ggtttttgtt
     2281 aatatcagag tgatttctgt ttattctcat ggagtttatg gggtgtttcg tattctggta
     2341 agttaatcaa aagggaggat ttcgagtctt ataaggtagg atatcttttt gtttttatcc
     2401 ggatttccat tttgagcgtt ttacctcctg ctttttctat tagaaaaact ctggactctt
     2461 ccttctcttt tattaattaa tccaagatca acgcatgagc caaacaaatt ctcaaccgct
     2521 cacttgctaa atctgcctct ttcattttat tgctgcgttg caaatgtttc tgtgatgctt
     2581 atttactctg gcaaaccctg ctcatcaaaa catcagtgtt ttccctcaag caaaataatc
     2641 agatgatgca agagagctaa taactgtgcg catacaattg gttattaatt tttcttaaag
     2701 atcaaataaa tagcaattct gggacttgtc tctttaatcc ataaaaaaaa ttgctccaaa
     2761 agtggtattt cagcttgacg tgtgtagaaa tgtgttccta tagtgtagaa aagtgggtta
     2821 aaataataaa aaagtggaga attgtgggag aataattctc cttcacttgg gaaataatat
     2881 gtttcgtgga attaatgcca tcaccataga cactaaaggg cgcttagcta ttccgacgcg
     2941 ttacagaagt gccttgggtg cagaggataa aattcctctc gtggtgacta ttgataccga
     3001 agaaacctgc ttactccttt acacagcagc acaatggcaa atcatcgaag ataatttgca
     3061 aaaactgcca agttttaatg ctgcagcaag acgaattcaa cgtctgttaa tcgggcacgc
     3121 cactgatgta gaggttgatg cgaatggacg agtactttta cccacagttt taagaaatta
     3181 tgcaaagctg gaaaaagatg tcgtgatgat aggccaggga aataaatttg aggtttggaa
     3241 taaagaatta tgggaatcca agcgtgagca atggctggca gaagaagcgt ccatgacgga
     3301 tgggttaccc gaggaaatga aaacgttttc tttataacgg aaagagaaat ggcaaagcat
     3361 caatctgttt tattacatga atcaatcaaa ggtttggcta taaaagctga cggtatctat
     3421 tttgatggaa cttttggccg aggaggccac agccgcgaga ttttaaatca tttaagcgat
     3481 aaaggaagat tgtttgcaat agataaggac cttgatgcgg tgcaatatgc caaggattat
     3541 tttggtttgg ataaacgatt tcaaattttt cacggttcat tcgcgcaaat caaggaattt
     3601 gcgtcacaag cgggggtaat tggggccgtt gatggaattt tgcttgatct gggagtgtct
     3661 tcacctcagt tggacaatcc agaaagaggt tttagtttta tgctgcaagg ccctctcgat
     3721 atgcgaatgg atttaactca atcaattaat gctgcgaatt ttgttaatga agcagaggtc
     3781 aacgagctgg ctcatgtatt cagggcttat ggtgaggaac gatttgcagg caggattgca
     3841 aaagctattg ttgatgcaag aaaactaaaa ccaataacaa ccactttgga gttagcggaa
     3901 attgttaaag aagcaaaccc caaatgggaa aaacacaaac atcctgcaac ccgggtattt
     3961 caagcgattc gaatacatgt caatcaggaa ttaaccgatt tgagtaattg tctagagcaa
     4021 tgtctggacg tgttggggcc aggaggacgc ttggccgtta tcagtttcca ctctttggaa
     4081 gaccgaatag tcaaacaatt tatgagagat aaagagcaag gtaacaggcc acctgttgaa
     4141 gttccaatca aatacgaaga actaaagacc aattttaaaa aagtaggtaa agcagtgaaa
     4201 ccacagagta gcgaaataaa ggaaaatgtt agatctcgaa gtgcagtact taggataggg
     4261 gagaaattag catgaacgct gcagcgaaag tcattaatca aggtaattta tttaatggtc
     4321 aattggcaga tatgcaaatg tcaaaatcac tctatatgtt gatagtattg ttagctgccg
     4381 tactagtcag tgcttttgcg gtcatatata gcactaattc ctacagatta actctcagtc
     4441 aagtgcaaca agaggaacag caaacccatt ttttacaatt gcaatgggga caattattat
     4501 tggaacaggc cagtttggca actccagccc gggttgagga attagccaga gaaaaactgg
     4561 gaatgacatt accaacttct gcaaatacgt acttactgca tcctcaataa gtgagtcgtt
     4621 ttggagaagt taaagtgttt tcatattttg agttataggt aagaactaag tacctggtga
     4681 aggataatct atgtccaaga gcatcaaatt aaagattgaa taatgaaatt tcaaaggatt
     4741 tgatagagca attagcgcaa tgcggaggaa atcacctcga aaaaattgaa agcttcatgt
     4801 acacttacac gtcattcatc ttctttccag gacgtcatgg aagactaagt gtaaaatggc
     4861 ctgataagtt ccaggcaggt atacgtgcct tatgtttaac tctgaggtaa ttttgaggaa
     4921 gaggtttcaa gtatattttc actaatttct tgttgcgtgc atagttaata ctatttatga
     4981 gtagtcatat atgaaaaaaa cacaccattt tgccagactg gttactgtcg ctgttttttt
     5041 ctctttgatt ttggctattt tgatatggag aatggttgat ctcactgttc tgcatagaca
     5101 atttttgcag ggccagggtg atgcgcgcag catccgagtt atagatattc ctgcctatcg
     5161 aggtatgatt accgatagaa atggtactcc gcttgctgtg agcacacctg ttgaatcagt
     5221 ttgggtcaat ccaaaggaat tttctccgga tgaagaacaa tttatgcagt tagctaaata
     5281 cctgaatttg gcacctaaag aattaagtag aaaaattgtc cgggctgaaa atcgggagtt
     5341 tgtttattta caaagacagt tacctccacc cctttcgaaa aagatcgaag ctttaaaaat
     5401 cccaggtgtt aattttcaaa aagaattcaa acgttattat ccagattcag atagtatttc
     5461 cccattaatt ggatttacca atgtcgatga tcaaggattg gaggggattg agctggctta
     5521 tcaagattgg cttatgggag tcgttggtaa aaaaagagta atcaaagatc gattaggacg
     5581 agttgtcgaa gatctgggag ttttgaagga accacgcccg ggacatgagt taactttgag
     5641 tatagataga cgactacaat atttagctta tagcgaatta ggtaaaacag tcgaagagtt
     5701 ttctgctaaa tccgggtctg tagttgtaat cgatgctgaa aatggtgaga tcctggctgt
     5761 agctaatgtt ccatcttata atcctaacgc acgtgggcat tatgataagg aaacctaccg
     5821 caacagagct ttcaccgata ctttcgagcc tgggtctgtc attaaatcat tcagtatagc
     5881 cagtgctttg gaaacaggct tatttactcc ggataccatt attgatacta atccaggatg
     5941 gatgactgtc catggccgaa cagtaagaga tatccataat tatggtgttt tagatgttac
     6001 cggaatatta caacattcca gtaatgtggg tgttacaaaa atggtattat ccactccagc
     6061 tgaacaatta atcgggtttt tgcaaaaatg tggttttggg caacgctctg aattggcata
     6121 cccgggtgaa agtgaaggag gcatagtaaa agccaaggat gcaaacccat tcgttttagc
     6181 tacattaggt ttcggttatg gcttgtctgt tactgcactg caattggcta aagccaattt
     6241 aatttttgca aataaaggac gattaatacc tgtgactttg attcataatg atacccccag
     6301 taatggcatt caagtgatgc aacctaaaac cgctgaaaat gtattattaa tgatggaagc
     6361 ggtaatgaat aagggtacag gtaagggagc aagggttcct ggttataggg tagctggaaa
     6421 aactggaaca gcacgagttg ctggaaagaa tggatataaa gacaggaaat acacagccag
     6481 ttttattggg atagcaccag tttccaaacc aaagtttgta gtcgctgtta ttattcatga
     6541 accttcacga aagggttatt atgctgcctc tgtggcaggg cccttatttg cgaaagtcat
     6601 gtcaggtgct ttacgcttat tcaatattcc tactgatgag ccagttagtt aaagagaata
     6661 ggagccaatt ttgtgaaatt aacccaatta ttagaaccgt ggattaaaag ggacattatt
     6721 gatggcactg tgagtgacat aaaaaatgac agccgcttgg tgaaagaagg ggatttattt
     6781 gtcgcctatc caggggcagc ttccgatggt cgattatata tagaaaaagc cgtggcatca
     6841 ggtgctgtcg ctgtggttta tgagcccaat aatttacctc agggaattac tttgccaaca
     6901 ggaattccct gtatagccat tccccaatta gctgaaaaat taggggaaat agcgaaacgg
     6961 ttttttaata acccatccca atttcttggt ttaactgggg taactggcac aaatggtaag
     7021 acgactattg cttatcagtt ggcacaggca catgatttac tcgggcagca ctcggcttac
     7081 atcgggacta tagggcaagg aaaggtggat gccttaaaac ctttggaaaa tactacgcca
     7141 gatgctcttt gtttgcagag gttaatgcat cagtataaag aaggaggcat taaacaggtt
     7201 tgtatggagg tatcctctca tgcgctgagc cagcatagag tcgactctct cgaatttagt
     7261 caggctatct ttaccaattt aacgctcgat catttagatt atcaccagac aatgcaagct
     7321 tatggggctg caaagtcgag gttgtttgaa cgagcagagt tgcaatgggc tattattaat
     7381 caggatgatg actttcaaga tctcatgagc aatgtgctaa agactcatgt aaaaaaagtg
     7441 acttacggta tgcatcaaaa ttgcgatgtg aaagcgctcc aatggaatat ggatatcacc
     7501 ggaacagaaa ttgaagtcaa atctccttgg gggcaccacc ttctaaagat taaagcactg
     7561 ggtaagttta atatttataa tagcctggct gtttatagca gtttgcttgc cagcgggtat
     7621 aatccagaaa aagtagtgca agttatggcc gaattaaaag cggctcctgg ccgaatggaa
     7681 attgtcgctc aatccccata tgttcttgtt gattatgctc atactcccga tgcattggaa
     7741 aatgttctta tcaccttaaa acaaataaaa aaagggagat tgtgggttgt atttggttgt
     7801 ggtggggaca gagataaaac caaacggccc attatgggaa aggcagccag catgtacgct
     7861 gataatattg tcattaccag cgataatcca agaagtgaag atccggaaat tattctaaat
     7921 gaaattgctt gtggcattcc tgcctctgcg cctgtctcta aactggtgaa tagagaagag
     7981 gcaattgctt atgctttaag aaaggctgag cataacgata tcatattaat agcaggaaaa
     8041 ggacatgaat cgtatcagca gattgggaat acaaaacatg ttttttctga tcaggaagtt
     8101 gtaagacgtt tgatgaataa ttaaaatttc gggacagggg ttaattatta gaaacagaca
     8161 tcaacacctt aacctaataa tggatacttt tttattgagt cagaatcaaa ataagttgtg
     8221 aaatcatggc ggtgatgatg attcttgcga agagtcaaga atgctgagga tagatttcca
     8281 gcgtgtttta attctagtgt ttcatgaatc gggttatata ttgataaagc caactctctc
     8341 cagtcgctgt tttcatttaa tctataaggt ttttcagggg ttgcaaattg tgggtatgga
     8401 agaccaagca tttgatgtaa tttacgactg acgattgcga ctgcagcaaa tttagcttcg
     8461 aggctgtgtc ctgcaatatg tggtgtgcaa agagttgcct tggagacaat ccggctatct
     8521 atatgaggtt cgttattgta gacgtcggtg caataaagta tggcagagcc aagatgcaaa
     8581 agggcttctt cattaacgat acctcctcga gaagcattga tgataatgca gccaggtttt
     8641 aattccttga gaaaatcccg attaattaag ttaaggcttg ggtagggcgc atcgctatgt
     8701 aattcagcat gaacacataa gaggtcacat tcaaaaagat cttcaagaga gcagctttga
     8761 aaagaagtat ctctggttgc tttgggtggg tcataaaggc ataactgaaa ttcggcagca
     8821 tttagtcgtt cataaacttt agtacctact tgccctaaac caataattcc tgctgtttta
     8881 ccttgaataa gttgctgttt atctaaatag gcaaggcaag caacaacata gtcagcaacc
     8941 gatatggcat tgcatccttt ggcatcaata atagatatgt tttgtgattc taaaaaagga
     9001 aaatcaatat gatcggttcc actggttgct gtcgcaacaa actttatttg gtggtttttg
     9061 agtaaatcgc catttatttt tagggttgaa cgacaaagaa gaacgtcctt atagtgaagc
     9121 aattctggaa tttcatcggc cttatgatac aaagttacgg taaatggtgg tggaaaagca
     9181 ctatctaatc ctggcaataa agcatctgct aaaatgttca taaattaaaa atcgcgctta
     9241 aaatatataa tgaaccatag atgaggggag taagaatata ccccaataca ttagtaaaaa
     9301 ttagtacaat caatattaaa aaaccaaatg gttctaattt tgcataagcg atagcttgtc
     9361 ttgctggtaa tagatttatt acaactttgc tgccatcaag tggaggaatg gggattaggt
     9421 ttaaaaaggc aagaatcaaa ttgatcatca ttcccgctct agctgtaaca agcaggaata
     9481 aagcagtcat tgagctttga gggcttaaca gcgttgctat tttaaaacat ccagcccata
     9541 ggaatgccat tatcagattt gctaatggtc ccgcaagcgt taccaatatc atatctcggc
     9601 gtggttttcg cagttggctc tggttaaccg gaaccggttt tgcgtaacca attacgaaat
     9661 tgaaatttgt tatcacgcca attaaaagag gtaatagaac agtgcctatt ggatcaatgt
     9721 gtttgatagg gtttaatgtt aatcgaccga acattttagc tgtgttgtca ccgcagcggt
     9781 aggcaacgta accatgagct aactcatgaa aagtaatagc taataatact ggtaaagccc
     9841 aaatacaaat ttgttggatt aacgtgaatt ctggcattaa tattctgatg tatttaataa
     9901 ggatcctgat tttaacgaat tctactcgct aataacaagg gttgatgaaa aattgtgtgg
     9961 ccgctcgcat ttgttgatat ttttaccatc cttgccgaaa aaccttcatt ttctactcaa
    10021 gcatgagaaa tctcgacagg acctaatgtt ttattaataa catggggtct tggctaaatc
    10081 gaaagcatct tatatgattc catttgatat gaggatttat aaggcttggt ttcgatgacg
    10141 caatttgaga aaacttttaa cttcatgaaa aaaatctggg taataataac ctatattat
//
LOCUS       DAXLDT010000109         9767 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXLDT010000109 DAXLDT010000000
VERSION     DAXLDT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.109"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            150..1799
                     /locus_tag="ACF2SP_002196"
     CDS             150..1799
                     /locus_tag="ACF2SP_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HFD2359972.1"
                     /translation="MKKLMLWVLLSLFSSLSFAEIKVELEPSQVTMGETFKLSITQED
                     QQGGGVPDLTELQKDFVILGTERHVNYSIINGQAQSQSQWIVLLRALKPGIFTIPPIK
                     VGLEQSLPITINVQGSANTQALPDEAIQKQDVFLTAEVNEKNPYVNQQIIYTVKLYNS
                     KRLLDADYQGPKVDDALLIPLGDAKRYHAVHNNINYVVEEQTYAIYPQKSGTLKIISP
                     IFNALVYDYNPQRTKATDKAINLTVKPIPKKYQGKLWLPAKQVKLSEQYENSNQTLSQ
                     GSTLTRTVTLEGIGIPAQLLPTLTFQETDFFSVYPEKGAERNQVKQGELIGSVEIKVT
                     YLFNKAGKTTIPELRLPWFNTETGKEEIAILPPRSIDIIPSTAVKANTANTQVTGKEM
                     SASSQSAPDSASVSNESGGWAVKLAIFFGLAWLITLSLWIWQKRPRESSSKKEYKKAL
                     DALNKACNQCNPKKARDAVLKWASLQWPDAPMLNLTDLAQLVRDAHLRKQLHLLSQVL
                     YRSQEKTLWRGDELLRAVQAVKRNESVKKRKNSKLPPINPF"
     gene            2036..3418
                     /locus_tag="ACF2SP_002197"
     CDS             2036..3418
                     /locus_tag="ACF2SP_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFD2359973.1"
                     /translation="MHVNEYIHYDVQELAELIKNHQVGAEEVLQCAQERLYQVNPSLN
                     AVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFANNLAETNS
                     DLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIM
                     AGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPWVDEAWSGMATSFILTRSM
                     SDAIALFKNVINPMRLNRLSRKKKKLLITELTGAFAEVPIARECQEAVDKIKEILKNS
                     GHRLNEVHLPLDLQVISSCAHTLIAASTYAKVKTQEQISGRKPMIHELEPITWEFYYR
                     GKMVSAYEFIIAKNKLYQLLRPLHQLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYL
                     QKKKEFSPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVL
                     PKNITPASIP"
     gene            3699..5156
                     /locus_tag="ACF2SP_002198"
     CDS             3699..5156
                     /locus_tag="ACF2SP_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2359974.1"
                     /translation="MKTLPSKVIFIGAGPSGLFAARQLKEIAKLQNKTVECIFLEREP
                     VVGGKCHTYSDAVHPELKTEWGAALVAPNYGVVLDALAEHDIKFEKVMPTASETIEIK
                     QLFEKASSLEKISMEMKLLRELWAFNSDYDVYKKAKQNKKALPDKLLSPFSEYCKLSN
                     MSYLPVLLKPFVPGFGYGDLNFCPAYCVLEYMGKVTMPDILLADQLIGRPSLLAIHGG
                     FQLLMERIAEQFDVRTSASVSRIKREKNKVTVTYVHNGIEQTETADTLVLATSPKNWK
                     SLGLDLTEVENNCIESLDYYRYPVAVYKIKGLPPQQYFFTKALEERGFGHLALITTRD
                     NRSNPEDGRLCTVYVNLSPNNNEFKFDHEALRKELESIPDVTEVTVVKDKIWEDYMST
                     LPWDLRLQLDKEQQRTNTMYLGSYALGSFEDVACVANKATDAMTEWYAPTMRYEEDFS
                     WKNMNRAWQFFTSPTKSPLASLGEPRMSDEHSNRP"
     gene            5395..5610
                     /locus_tag="ACF2SP_002199"
     CDS             5395..5610
                     /locus_tag="ACF2SP_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD2359975.1"
                     /translation="MSVKETGHVKWFNDDKGYGFISRDNGQDDVFVHFRSIVSSAGRK
                     TLVEGQRVEFLVTKGAKGLQAEEVTAI"
     gene            complement(5711..7015)
                     /locus_tag="ACF2SP_002200"
     CDS             complement(5711..7015)
                     /locus_tag="ACF2SP_002200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2359976.1"
                     /translation="MTQQDPVVIKSKGDQALEKGDLPLALTLYDEALAINPQFSGAYY
                     QKGTVLLRMGKAIEAAAMYWQAYLFSEFKADIGLMTAKTLAHANYSLSACKLFESFKI
                     EEMDGESLAYYLYALRQQGRVQEAYSLFPYVNQFNIPKTSWARAIILLDLNKVEEARF
                     LLEPLEKTDKDGHITILLHAVYLALNDKKAVKSLLDRAIQRIPNNDYFCCQRIAIDIL
                     NGLNKTPIETYRAFKRFDLIDAADYLHKHADKHLRHSGTTYQTFDLLAPQVLSEGLIL
                     EFGVRFGYSISHIAKLFPKRKIFGFDSFQGLPEDWHHEKAGSYSTYGKIPSVANNVTL
                     IAGWFNETLPDFKKNNREPIAFMNIDCDLYSSTKLVFNELNSQIAPGTIIVFDEYIGN
                     INWRQDEFKAFQEWVKENKVEYRYLTASFYTKQVSVQILKRK"
     gene            7178..8158
                     /locus_tag="ACF2SP_002201"
     CDS             7178..8158
                     /locus_tag="ACF2SP_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFD2359977.1"
                     /translation="MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNP
                     DNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLRWVSVDCGVIEYTERPKASCDLS
                     PGMADSYVLALSSQTGFCETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHN
                     YGYCGTSPRTNHCDYSPLDLSSDVAQNLKKLMPSYNYGSCLERHEWNKHGSCQDLSTD
                     GYFSLAMRLTTEVNNTEFGQYLTAHRGDTVKLATLRELIERSFGENNTGKVTLSCKNG
                     ILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNNNVTISDFSNEKWL"
     gene            8297..9259
                     /locus_tag="ACF2SP_002202"
     CDS             8297..9259
                     /locus_tag="ACF2SP_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HFD2359978.1"
                     /translation="MLKRCYLLILLMFVLASCAHHKPQTPPADVKKQGTSSTRQFRQV
                     SSFNQIVVQGRLNVNLHTGYNKPEVMLRGDPRDLVQVRTIVKQNTLYVSLGQGYPDYG
                     TVTVDIKTKFLNRFRYEGAGVVTGNNIRTSYLDLYLANEGTTRLAGNIGLQKLEAVGS
                     GVTQINGVSSRNLQIVLKGDPKVLISGFVNLRQLDMYGKGTLSLYWIKSDTLTIRAKK
                     AAKIQLAGIVNRLDVELWDFAQFKGKYLRAQRSFVKTHDKSVAEISAVNHQSSLATDA
                     SDIYYYNLSKTRADFMAFNGSVLDMREWSQSDLKDFDRYNKQFP"
     gene            complement(9519..9752)
                     /locus_tag="ACF2SP_002203"
     CDS             complement(9519..9752)
                     /locus_tag="ACF2SP_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359979.1"
                     /translation="MFFTKTSLKNDDLNVSKIAIFVAGLASLLIIGNYNDGDTYEEVS
                     PSFLSQIMNDPPNYPFTSDEEIFEVNIGLPFRF"
BASE COUNT         3023 a         1738 c         2045 g         2961 t
ORIGIN      
        1 aagcaagagg gagaaattca gtccgaatcg aatcaggaga aacaacttgc taaagaacaa
       61 tggttgcgat taatacctga tgatccgggt ggattaatga gagaaaaatt tttacgtgat
      121 catttacgta gagagcgtgg gtggtatcaa tgaagaaatt aatgttatgg gttttattga
      181 gtttattttc atcattatct tttgcagaaa tcaaagtcga acttgagcct tcgcaagtca
      241 caatgggaga aacctttaaa ttatccatta ctcaagaaga tcaacaaggg ggcggagttc
      301 ccgatctgac tgaattacaa aaggattttg tgattttggg tacagaaagg catgtaaact
      361 attccattat taatgggcag gcgcaatcac aaagtcaatg gattgtactc ttgagagcgc
      421 taaagccagg tatttttact attcctccaa taaaagtagg tcttgaacaa agtctgccaa
      481 tcactatcaa tgtccagggt agtgccaata cacaagcttt gccagatgag gctattcaaa
      541 agcaggacgt tttcctgaca gctgaagtga atgagaaaaa cccctatgtt aaccaacaaa
      601 ttatttatac ggtcaaatta tacaatagca aacgattgtt agatgccgat tatcaaggtc
      661 caaaagttga tgatgccttg ctgattccgt taggggatgc gaaacgttac catgctgttc
      721 ataacaacat taattatgtt gttgaagagc aaacttatgc gatatatccg caaaaaagtg
      781 gcactttgaa aatcatatca ccgattttta atgctcttgt atatgactat aacccccaac
      841 gcaccaaagc gacggataaa gcgattaact taaccgtcaa gccaatccct aaaaagtatc
      901 agggcaaatt atggttgccc gcaaaacaag ttaagttatc cgagcaatat gaaaacagta
      961 atcagaccct cagtcaggga agtacgttaa cacgtacagt gacattggaa ggaataggca
     1021 ttccagctca gttattgcca acacttacct ttcaagaaac agatttcttt agcgtttatc
     1081 cagagaaagg ggcggaacgc aatcaggtta agcaaggtga gctaattggt agtgtcgaaa
     1141 tcaaggtcac ttatttgttc aacaaagcag gcaaaacgac aatacctgaa ttgcgtcttc
     1201 cctggtttaa tactgaaaca ggcaaagaag aaattgccat tttgcctcca aggagcatag
     1261 atatcatccc ttctactgca gtcaaggcaa atactgcaaa cacccaagtc acaggtaaag
     1321 aaatgtctgc cagtagtcaa tcagctccag attctgcatc agtttccaat gaatcaggag
     1381 gttgggctgt gaagctggca atattttttg gtttggcctg gctaatcact ttaagcttat
     1441 ggatatggca aaagcgaccg agagagtcat ccagtaaaaa ggagtataaa aaagctttgg
     1501 atgctttaaa taaagcttgt aatcaatgta atcccaaaaa agctcgtgat gctgttttaa
     1561 aatgggcctc tttgcaatgg cctgatgctc caatgcttaa tttaactgac ttggcgcaat
     1621 tggtaagaga cgcgcattta aggaaacaat tgcatctgct ttcgcaagtg ttgtaccgaa
     1681 gtcaggaaaa aacattatgg cgcggggatg aattattacg ggctgtgcaa gcggtaaaac
     1741 gtaacgaatc tgtgaaaaag cgaaaaaaca gtaaattacc tcctattaat ccattttaga
     1801 aattaaatct atttaagcga tggtttttgt cagttattcc tttactgtca atatagacaa
     1861 cttatttatc ctcttttttt tgtaatcacg agactactgc cagattgtat gccgtcataa
     1921 tttggttaag ctggctcagg cgactatgat aacaacagct ataatgttat agataacctt
     1981 gtaaagagta atgttctctc tttataaagc tctctggcta ttgtaggaat tgagtatgca
     2041 tgttaatgag tatatccact atgatgtgca ggagctcgct gaactgatca aaaaccatca
     2101 ggttggtgct gaggaagttt tgcagtgtgc gcaagaacgc ctatatcaag tgaatccttc
     2161 attaaatgcc gtagttacag attgttttga atatgcgcat caatgccttg cgaaaatgaa
     2221 aggtaacgag ccctattatg gtgttccgtt attagtcaag gatctgggtc atgccataga
     2281 gggcattcga ctaacggaag ggtcaaggtt ttttgcaaat aatctggctg agaccaacag
     2341 tgatttgatt aataaattga ttgctcttgg ttttgtacca tttgcaaaaa caaatacttc
     2401 agaattaggc ctttcctgcg ttactgactc taatttattt gggccttgca gaaatccttt
     2461 tgatactatg agaacggcag gcggatcttc tggtggttct gctgctgcca taatggcagg
     2521 gatagcgcct gtggctacgg caagcgatgg aggcgggtcg attcgtattc ctgcggcgtg
     2581 ttgtggtttg tttggattca aaccgacacc aggtctggtt ccctcagggc catgggttga
     2641 tgaagcatgg tccgggatgg ctaccagttt tatactcact cgttccatga gtgatgctat
     2701 tgcattgttt aaaaatgtaa taaaccccat gcgacttaac agactatcga gaaagaaaaa
     2761 aaaattgcta attaccgagt tgacaggcgc ttttgccgag gtaccaattg caagggaatg
     2821 ccaggaggcg gtggataaaa taaaagaaat cctgaaaaat tcaggtcata gattaaatga
     2881 agtgcacttg ccattggatc tacaagttat cagctcatgc gctcatactt taattgcagc
     2941 gagcacgtat gcgaaagtaa aaactcaaga acaaatcagt ggcagaaaac caatgattca
     3001 tgaattggag cccataacat gggaatttta ttacagaggg aaaatggtct ccgcttatga
     3061 gttcattatt gccaaaaaca aactatacca attactcaga cctctgcatc aattattgga
     3121 aaaaacagac attgtattaa ctcctgcatt ggctcaatta cccctgttaa tcggtcattt
     3181 gcgaactgat gaggaattca ataagtatct acaaaaaaag aaagagtttt caccttttac
     3241 ctccctgttt aatcaaggtg gattacccgc tatgactgta ccagttatgt tccaccatca
     3301 tttgcctgtc tctgtgcaaa tgggttcagc aaaaggcaat gatttgctct tattggagtt
     3361 ggctcatgaa ttacagtctg tgctgccaaa aaatatcacg cctgcatcaa ttccttgatg
     3421 ctgatttttt gtaagttttc atttatcgaa ccttgtgtta agttaactgt agcttttcat
     3481 aaaattaaga ataagtgatt gttttttata gctatagtca cttgaatttt aaataatcga
     3541 ttctctacgt actgctgctt aatgcggttt ttaaaaatca agttactata tattttttca
     3601 acttcagggc attttgcgta ggcagtaaat agttccagtt tgagtagttg tatttaataa
     3661 aggtaagttg taaatggatt tcactaaaga ggatttcaat gaagacatta ccatccaaag
     3721 tcatttttat aggggcaggc ccgagcggct tattcgctgc acgtcaatta aaggaaatag
     3781 ccaaattaca aaataagaca gtagaatgta tatttcttga acgtgaaccc gtagtgggtg
     3841 gcaaatgcca cacctactcg gatgcagttc atcctgagtt aaaaacggag tggggagcag
     3901 cattggttgc tcccaactat ggtgttgttc ttgatgcatt agctgagcat gacattaaat
     3961 ttgaaaaagt aatgccaacc gcttctgaaa cgatagaaat caagcaatta tttgaaaaag
     4021 ccagttcgtt agaaaaaata agcatggaaa tgaagttgct tagagaatta tgggctttta
     4081 atagtgatta tgatgtttat aaaaaggcaa aacaaaataa aaaagcactt ccagataaat
     4141 tgttaagccc cttttctgaa tattgtaagc tgagcaacat gtcttattta cctgtccttt
     4201 taaagccatt tgtgcctgga tttggttacg gggatttaaa cttttgccca gcttattgtg
     4261 ttcttgagta tatgggtaaa gtcactatgc ctgacatcct gttagccgat caattaattg
     4321 gcaggccttc actattagca atacatggcg gttttcagct tttaatggag agaatcgcgg
     4381 aacaatttga tgtgcgtacc agtgcttctg tttccaggat taagagagaa aaaaataagg
     4441 tgactgtgac ttatgtgcat aatggtatcg agcaaacaga aacagccgat actttggttt
     4501 tggcgacttc accaaaaaac tggaaatccc tgggtttaga cttaactgaa gttgaaaaca
     4561 attgtataga gagtctggat tattatcgct atccggttgc tgtttataaa atcaaaggat
     4621 tgccgccgca acagtatttt ttcacaaaag cacttgagga aaggggcttt gggcatttgg
     4681 cgcttattac aaccagggat aacaggagta atccggaaga tggacggtta tgtacggttt
     4741 atgtgaattt atctcccaat aataatgaat ttaaatttga tcatgaagca ctaagaaaag
     4801 aattagagtc tatcccggat gtaactgaag ttacagttgt aaaggataaa atctgggaag
     4861 attatatgtc gactcttccc tgggacttgc gattgcaatt ggataaagaa caacagcgca
     4921 cgaatactat gtatttgggc tcttatgctc tgggaagttt tgaagacgta gcctgtgtgg
     4981 caaataaggc aaccgatgcc atgacggaat ggtatgctcc cactatgagg tatgaagagg
     5041 atttcagctg gaaaaatatg aatagggcct ggcagttttt tacatcgcca acgaaatcgc
     5101 cactcgcttc gttaggtgag cccaggatgt cggatgaaca cagtaacagg ccatagacgg
     5161 taatataaaa gtggtacatt tatcgaagat attgtacata atgctcgcta tttgtagaat
     5221 agtgtattat aatataatca cttgttctac gagcattatt ggttaaaatc ttgtttttcg
     5281 acaaactcct tccttatatt tcccttggtc gtgaatttct ctcgatgatt acattatatt
     5341 ttttctctta tcgctcagta caagtgtttg tttaatttta tttggaactt tgaaatgtct
     5401 gtgaaagaaa ctggccacgt taagtggttt aatgatgata aaggttatgg cttcattagt
     5461 cgtgataatg gtcaagacga tgtatttgta cacttccgct ctatcgttag cagcgctggg
     5521 cgcaaaactt tggtagaggg gcaacgagtg gaatttcttg ttactaaggg agcgaaaggt
     5581 ttgcaagcag aagaagtaac tgctatataa tcccgtattc actgaattac cttcctcttt
     5641 gtctcaaaac aacgaagagg gaggtggttt tttaatgcca ataaatcgca ttaatttttt
     5701 caattatttt tcactttctc ttaagaattt gtactgagac ctgtttggta taaaaactgg
     5761 cagtaagata acgatattcc actttatttt cttttaccca ttcttgaaac gctttaaatt
     5821 catcctggcg ccaattgata ttaccaatgt actcatcgaa tactatgata gtaccaggtg
     5881 caatctggga gtttaattcg ttgaacacca atttagtgga agaatagaga tcacaatcga
     5941 tattcataaa agctatgggt tccctgttat tttttttaaa atctggcaaa gtttcattaa
     6001 accatccagc gattaaagtc acattgtttg ctacggaggg aattttaccg taggtggaat
     6061 aggagccagc cttttcgtgg tgccaatctt caggtaaccc ttgaaaactg tcaaaaccga
     6121 aaattttgcg tttgggaaag agtttggcaa tatgggaaat ggaatagcca aatcgtactc
     6181 caaattccag tattaagcct tcagaaagaa cctgtggcgc taataaatca aatgtttgat
     6241 aagttgttcc actatgtcgc aaatgtttgt cagcatgttt atgaagataa tcagctgcat
     6301 caatgagatc aaagcgtttg aatgcgcgat acgtttctat cggtgtcttg tttaaaccat
     6361 tgagtatgtc tattgcaatt cgctggcagc aaaaataatc attattgggt atgcgttgaa
     6421 ttgctctgtc taaaagagat tttactgctt ttttatcatt taaagccaga tatactgcgt
     6481 gcagtaaaat ggtgatgtgt ccatccttat ccgttttttc aagtggttca agtaagaagc
     6541 gcgcttcctc tactttatta agatcaagca aaataatggc tcttgcccaa cttgttttgg
     6601 ggatattgaa ttggtttaca taagggaata aggaatacgc ttcttgtacc cttccttgtt
     6661 gccttagagc atagaggtaa tatgcgaggc tttcaccgtc catttcttca attttaaatg
     6721 attcaaaaag cttgcaggct gacaaagaat aattagcatg cgccagagtt tttgctgtca
     6781 ttaatcctat gtcagctttg aattcgctga ataaataagc ttgccagtac atggctgctg
     6841 cttctatggc tttacccatg cgaagtaaaa cggttccttt ttgataatag gctccggaaa
     6901 attgtgggtt tatcgctaat gcttcatcat acagtgttaa ggcaagaggc aaatccccct
     6961 tctctaaagc ctggtctccc ttggatttaa ttacaacagg atcttgttgg gtcatttatt
     7021 ttctccagta cctacctcta tatcatagta ttcttttatt agtaatgtag ttgtattaaa
     7081 aaaatcctta ttaatcaatt tcatgaaaaa ataattgctg gcattcatat ccgactaggg
     7141 tataatgggc ttaccaaaga caagccagga tgtcgtaatg ataaaaccaa tcaccctgct
     7201 tttgcttttt tgttctttca ctgttaatgc ctcaattctc gtcaatggta ctttcgaagc
     7261 aacaaaatca tgccctgctt acctttccaa gaatcgtaaa tcaaatcctg ataatttaat
     7321 ggttgagccc aaccagaaat atcaattaaa agaaattaat aaaaccaaac cggattggtt
     7381 gcgtatacaa ttttcagacc atcaacctaa tctgcgctgg gtgagtgttg attgcggggt
     7441 cattgaatac actgagcgtc ccaaagcgag ctgtgatctt tctcccggga tggctgattc
     7501 ttatgtgttg gctttaagtt cacagacagg tttttgtgaa acttatggat atgaaatagg
     7561 caagccagag tgtttgaaat taacaaagac agcttatcag gctaatcatc taatcctgca
     7621 tggattatgg cctaatcaag aagcatgcgg ccataattat ggctattgtg gaacaagccc
     7681 tcgaacaaac cattgtgatt attctccatt ggatttatct tctgatgttg ctcaaaattt
     7741 aaagaaactt atgccgagtt acaattatgg cagttgtctg gagcgccatg aatggaacaa
     7801 acatggaagc tgtcaggatt taagcacaga tggttatttt agtttggcca tgcgcttaac
     7861 tacagaagtt aataatacag agttcggtca atatcttacc gctcatcgtg gtgatactgt
     7921 aaaattggct actttgcgtg aattgattga aagatctttc ggggaaaaca atacaggtaa
     7981 agtgacttta agttgcaaga atgggatctt ggtggatatt tatgtccaat tacctgctct
     8041 catacctttt aaagagtctc tagagtcttt ggttgataaa gccagtcact cacaacacca
     8101 tgattattgt aataacaatg tcaccatttc tgattttagt aatgaaaaat ggttataggt
     8161 attcaagtag aggagatgac tcgttcacaa aattttcttt aaaataatat ggcattgctt
     8221 ccttatagga gactgccttg cacattgtca aaacttcctc taatgtagag agattattga
     8281 tataggaata cagattatgc taaagcggtg ctacttattg attttactta tgtttgtatt
     8341 ggcaagctgc gcccatcata aaccgcaaac acctccagcg gatgttaaaa aacaaggtac
     8401 cagcagtaca cggcagtttc gccaagtgtc atcgttcaat caaatcgtag ttcagggacg
     8461 actgaatgtt aatttgcata ccgggtataa taagccagag gtcatgttac gcggtgatcc
     8521 cagagatttg gttcaagtaa gaacgatagt aaaacaaaat acactctatg tgtctttggg
     8581 ccagggatac ccggattatg gtactgtgac tgtggatatt aaaaccaaat tcctcaaccg
     8641 ctttcgctat gagggtgctg gggttgtgac agggaataat atacggacca gctatttgga
     8701 tttatatttg gcgaacgaag gcaccaccag actggcagga aacattgggt tgcaaaaact
     8761 ggaagcagtc ggtagtggtg tgacacaaat caatggtgta tccagcagga atttgcaaat
     8821 cgtattgaaa ggagatccca aagtactgat atctggtttt gtcaatctaa gacagcttga
     8881 tatgtatgga aaaggcacgt taagtttgta ttggataaaa agcgatactt taaccattag
     8941 agcaaaaaaa gccgccaaaa ttcaattggc tggaattgtg aatcgactgg atgttgaatt
     9001 gtgggatttt gctcaattta aggggaaata tttacgggct caacgtagtt ttgtgaaaac
     9061 ccatgataaa tccgtagcag aaatttcagc tgtgaatcat caaagcagcc ttgctacgga
     9121 tgccagtgat atttactatt ataacttatc aaaaaccagg gctgacttca tggcttttaa
     9181 tgggtctgtc ttggatatgc gcgaatggag ccaatctgat ttaaaagatt ttgatagata
     9241 caataagcag tttccctagc attgtaactg ttcatggggt atgtaattaa ggaatattag
     9301 gtcctctgtc tttcccgcga aggcgggaat ctatccataa atctagcaca agggttgatt
     9361 ttgagttggc ttcccgcctt cgcgggaaag acagctaaaa taaataggct tataaacaga
     9421 aatatttgca taccccgtgt acggttacct aacatttatt caggataaga gagctaaatt
     9481 cattagggta atcccttggt gaatttagct ttgtttgatt aaaatcggaa tgggagccca
     9541 atattgactt caaatatttc ctcatcagag gtgaatgggt aattaggggg atcgttcata
     9601 atctgactca gaaatgaggg agatacctcc tcataagtgt ccccatcatt ataatttcca
     9661 atgattaaca acgaggctaa tcccgcgacg aaaatggcaa ttttagagac atttaaatca
     9721 tcatttttta atgaggtttt agtaaaaaac atagttaatt cctaaaa
//
LOCUS       DAXLDT010000110         9668 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXLDT010000110 DAXLDT010000000
VERSION     DAXLDT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.110"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            317..1123
                     /locus_tag="ACF2SP_002204"
     CDS             317..1123
                     /locus_tag="ACF2SP_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359980.1"
                     /translation="MDMREKYTGRSEPIINKPSDIPKGLVAKRKAELLLAKLEEAIKA
                     QESLNRESESPAKEGNSESLKPRLTHLTSERPKINHRPPSSFSYTNSDSTLFTSVKEE
                     KTEGEVSSVSLSNDKKKVEDNSSASIWTELIAALSRAVDNYTKKKMEGTTPGEAYGWF
                     SWLRHGKAGEDKAGGLASEARTTSTVESLLTLLHHFFADSTTRYNNHSLAPYVMDELN
                     KLLIQYALKPFILSLGSQYDLNSWLHIKNQLTQLAGKEACQEKQSLVAPV"
     gene            1410..1964
                     /locus_tag="ACF2SP_002205"
     CDS             1410..1964
                     /locus_tag="ACF2SP_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359981.1"
                     /translation="MKTKGISCAVLLLVGTMLAEIGHAEFNENSNSIVATNNSQNTSE
                     HSSQQNLKRSPSGQNCTGTSPNPFPQNPASSPFSIGQLGACVNSNACNATNATLKSKI
                     ADRYCQDANKATCDANDDCLPPPDCKGRVNGTASSIVLANCQNAGPSLCASSGGGIYC
                     LCDAEIAANAQIKCGCECTTKTLP"
     gene            2299..3642
                     /locus_tag="ACF2SP_002206"
     CDS             2299..3642
                     /locus_tag="ACF2SP_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359982.1"
                     /translation="MKEIIELLATLDELQKPYIHRVKTRLSELLNLDEKENELIRIEQ
                     STSRSWEEGATTDLRIGPIKSKKRIEEIRAVLIALSGANAVKYEEGHNLENNAVEFIV
                     TACDVEQLEGFIPLNQEEMQNTIPVMTFIRMSIAGNRGYVRGISFNSEKLDGENYLSS
                     FKEGELTTSTQEWFSVLELLDLLNSESELNLIQIQNDLKSLLGVKFEVYFHSSSEEFP
                     IDLRTGSLDLKEAGAQQLLRSMDSLVLLDCLKKENKELEDGQKVCIIKQVNPQELHRF
                     SLKKGKNTNSSAEMLSNIIELLKRSFPNNQVIQDLVVFDGRDGRSMSRPYFHIRTQNY
                     HQEGTSDYKSAMSILKKLQDKTSEKIIFDFRNEMVEMKDESRPIYMSSIENTIKSSQF
                     IIDVAVGLTTQQLPELQNICAEINQSRLKHHRHNLFKPQIEEQKINEQNQSLFLG"
     gene            complement(3731..5425)
                     /locus_tag="ACF2SP_002207"
     CDS             complement(3731..5425)
                     /locus_tag="ACF2SP_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359983.1"
                     /translation="MKIILSILIALHATSTYAGQQVLVLSGGDNPGLNHYTQYLQTKT
                     LYEHLITRFNPETVSIYFGGGNTPTTQPSPFDVHKLINTKDNQQNKEDVMFTGIIANN
                     QQANKYNVDAFFLSSKIQQINPGDNLFLFVSDHGMPHGFLEDKNTNPYSNNCIDLWHY
                     QKPFINNFTNAKNFSKACLSKNELSSLLTLIPSQHVIFAMSQCYSGGFHQLSVTTQDG
                     YPVANTKICGFTSSTDDHYASGCTADANGATYQGYERSFTEWYTGHGVIDGKKIREPA
                     KSMLVAHQNAIIEDMTVDIPLSTSDYYLLQWAKLFASKQFKSRTKHYDNNMIQSIYLN
                     YKKRLPSSNNDALYQFIHVARASEDKIVQLMPEAREFASLSLTKQKQKISSLEKQLEQ
                     QGNELQDVWEGMMNLTLNVIMPSWEKAVNQSKSQPLNEKQQTFEKEFYQTIVKLNLYQ
                     HPYQFEMYYLQYLSEKQNDPDLVDYQKNRNAIIYEWARAHNKDPLANAIKNWEKLNKK
                     QLSLTEHIAEEEKKKQWLKRIFTYNQIIASWVTLLTIHDEKALADLEGLLKCQNAS"
     gene            5750..8011
                     /gene="lem25"
                     /locus_tag="ACF2SP_002208"
     CDS             5750..8011
                     /gene="lem25"
                     /locus_tag="ACF2SP_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem25"
                     /protein_id="HFD2359984.1"
                     /translation="MSTSEKDVREQKVKTVTLSFLGTGQHREKVHHILTSFHNTISEV
                     NKDNPTVAMRMFDGPGSEPKSGDSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQK
                     LTGNLAGEGIEHLLFEAVLYLNDIIEKNGGKLPDTVNLHGFSRGADTCMRMANLLYQL
                     YPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVEHFESTLMLHEYRPGFDPQHSGRYVI
                     ADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLHDDMNRFCRETGSLPSVGISPP
                     IIARVGDKKEEVRTHSELSPEKRFELLCGMKENEWGYAKLTKKYHERSILSKREDYVQ
                     DSRLFVNQEHRELFKQLYPKSFNWFFERNHGGQTKKEEVITELNSLSEDPRYEHFFSS
                     LAKHFQINENNIAGTLPEPSGIDRDEKRSFGQPPVRDRLSYLQHSLTSIANYYHYHCD
                     EKSSTNESVKNLLLERVKESRTKPDSEAIKHLEQTMDEVRQTLESKNEKGFLWQQINH
                     ISPNARQYCEQVKAALREHLEHNQVLSDTQKEEIRKAMDRMDNIVNDGSKDSQQKYRE
                     IRREVIELNAKATTPEDDNQLTRSHFQKAYFELSGDTQKTLNLESLSQTLNQLSKAHY
                     GETNMTDKITQRLDGYKNRNWFWNSVREVLNFFNIPIPKLHSEVKEQIADKLKERLVD
                     LKEKGMGNDVNAITRELGKAREDLIEHYKKTSKLEMGELDKIINKSMEELLVARKVTK
                     DLVHEEVSQVKLN"
     gene            8223..8861
                     /locus_tag="ACF2SP_002209"
     CDS             8223..8861
                     /locus_tag="ACF2SP_002209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HFD2359985.1"
                     /translation="MFTLKKNNSNIVSNLLNTNLGSFTRVPNEILLHVMYFLDLQSLS
                     RFARTNKDINYQVHELSKAFNVTLIDIGCEGTYSELHQKYSKFLVASQKRQKELQSIK
                     EVRESINQKRKDLEDNRANKSSCISKEEKTFTVVCCVLGTVGGSLFACCFPVNWGLSA
                     LIGGVSTTPIPLIASRTTKCVCQKCVECKAHSLELREQNLDNNFPPPSKMLK"
     gene            complement(9075..>9668)
                     /locus_tag="ACF2SP_002210"
     CDS             complement(9075..>9668)
                     /locus_tag="ACF2SP_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2359986.1"
                     /translation="NNALHFIKTAALENAENIDGKTISTWEHAAKQIAKTEQEEPERI
                     VLPKFENILTKHDTDPRQEILTLPDKTKVHISIMKIPVNEREENILAVYTVLKNDELL
                     RNQDGSVPTIIKDIRDMVGKIDPSKEENIANAIIEIKRKIAENKDNNYNQNAHDVIKA
                     FAKPSCCDFQKIRAALSANPAIEEIMNSVRVERQLKI"
BASE COUNT         3090 a         1652 c         1894 g         3032 t
ORIGIN      
        1 tagcttgact aaaccattgc aatctgcttt ttgcttcaat tgtcgcattt tgaaaacttg
       61 agttgtttaa aataaaaact gtgttgagcc attttgccat taaacattaa ttaatcttaa
      121 aaactaatca gattatgctt gctgaactac agaaattacg acttaatgtg taaaggcgaa
      181 tcttaaatag taaatattta atttcattct attgctcttt tctgatctga tagaagagat
      241 taattccttg aaagaaaaca atatattgca ttaacattag tgaactgttt aaattcataa
      301 atcatgacat agggaagtgg atatgagaga aaaatatacc ggtcgatcag agcctattat
      361 caataaacct tctgatattc ctaagggttt ggttgctaaa agaaaagcag agttgctctt
      421 ggcaaaatta gaagaagcta taaaagctca agaatcttta aatagagaat cagagagtcc
      481 tgcgaaggag ggcaattccg agtcactcaa accccgattg acccatctaa cgagcgaacg
      541 tccaaaaatc aaccatagac ctccttcttc ctttagctat accaattcag acagtacttt
      601 gtttacttct gttaaagaag aaaaaaccga gggggaggtg tcgtctgtat ccctttctaa
      661 tgataaaaaa aaagttgaag ataactcttc tgcatcaatt tggacggaac tgattgctgc
      721 cttatcaaga gcagttgaca actatacgaa gaaaaaaatg gaaggaacta ctcccggtga
      781 agcttatggt tggttcagct ggttgcgtca tggcaaggca ggggaagata aagcaggagg
      841 gctcgcaagt gaagcacgga caaccagtac ggttgaatct ttgttgacct tacttcatca
      901 tttctttgcg gattctacca ctagatacaa taatcattca ttggctcctt atgtgatgga
      961 cgagctcaat aaattattga ttcagtatgc tcttaaacct ttcatactca gtttaggctc
     1021 ccaatatgat cttaatagct ggctgcatat caaaaatcaa ttaactcaac tggcaggtaa
     1081 ggaagcgtgt caagaaaagc aatctcttgt tgcacctgtg tgattatcag gagtcctgca
     1141 caggactccg tattttttaa tcgcttgagt ttaacagttt aaattttttt gaattatttg
     1201 atgattgctt gattccaaag tttaactgtt caagactcga tcaatgctcg gtatttttaa
     1261 tgcttattaa aatactgttt tttgccaagg tatgcctgtt gatactttgc tttttattag
     1321 tgacgatatc cttatcaccc tgcaaacttg agtggatgaa aaaactatcc tagactttga
     1381 atagctgtcc aaacgtttaa gagaataaaa tgaagacgaa agggatttca tgtgctgttt
     1441 tattgctggt tggaacgatg cttgcagaga ttggccatgc tgagttcaat gaaaatagca
     1501 acagcattgt ggcgaccaat aactcgcaaa atacctcgga acactcgtct caacaaaatc
     1561 ttaaacgtag tccttcaggg caaaactgta ccggaacaag ccctaaccca tttccacaaa
     1621 accctgcatc gtctcctttt tcaatagggc aattgggcgc ttgtgttaat tcaaacgcat
     1681 gcaatgccac taatgcgaca ttaaaaagta aaattgcaga caggtactgt caagacgcca
     1741 ataaagcgac ttgtgatgcg aatgatgact gtctgccgcc tcctgattgt aaaggtcgtg
     1801 tgaatggaac agcttccagt attgtgctcg ctaactgtca aaatgctggt ccgagccttt
     1861 gcgccagttc tggaggaggg atttattgcc tttgcgatgc agaaatcgca gcgaatgccc
     1921 aaattaaatg tggttgcgaa tgtactacta aaacactgcc ttaatagcct taataaataa
     1981 attgtgaaga catcccctgt ctattttagt tggcaataca ggtatatcaa aaaaatctct
     2041 ttgagccagt ctctttcatt ttctgtacgt aatttgattc atttaaaata tcaccagtta
     2101 ttttaacaat tttcgtgctt cgttatggga gcatagagtt ttattaaaca gagtacatat
     2161 tgtatttaaa ttataaacat tgcgcttttt atatattgca ttttatgtta ttgttcaatt
     2221 tcttgatcaa taaaatcagg aagttagata caaaaactat tttggaatag tccttttact
     2281 ttacttggaa tttaaaagat gaaagaaatt atcgagttgt tagctacctt ggatgaattg
     2341 caaaagcctt atattcatcg tgtcaaaacc aggttaagcg agcttttaaa tctggatgaa
     2401 aaagaaaacg agctaatacg aattgagcaa tcgacaagcc ggtcctggga agaaggtgct
     2461 acaaccgatt taaggatagg tcctataaaa tcaaaaaaaa gaatagagga aataagagct
     2521 gtactcatcg cattgtctgg cgctaacgca gtcaaatatg aggaggggca caacttagag
     2581 aataatgctg tggaatttat tgttacggca tgtgatgttg agcaactgga agggtttatc
     2641 ccacttaatc aggaagaaat gcaaaatact attcctgtta tgacgtttat tcgtatgtca
     2701 attgcaggga atagggggta tgtgcgaggg atttcattca attctgaaaa actcgatgga
     2761 gagaattatc ttagttcgtt taaagaaggc gaattaacga cgtcgacaca agaatggttt
     2821 tccgttcttg aattattgga tttattaaat agtgagtcag aacttaatct tattcaaata
     2881 cagaacgact taaaatctct tttgggggtg aagtttgaag tttattttca ttcatccagc
     2941 gaagaattcc ccattgattt gcgtacagga agcttggatc tcaaagaggc aggggctcaa
     3001 caactgctga gatcaatgga tagccttgtt ttgcttgatt gtttaaaaaa agaaaacaaa
     3061 gaacttgagg atggccagaa agtatgcatt atcaagcagg taaatcctca ggaacttcac
     3121 aggttttcac taaaaaaagg aaagaatact aattcaagtg ctgagatgct ctccaatatt
     3181 atcgaacttt taaagcgatc ttttccgaat aaccaggtaa tccaggatct ggttgtcttt
     3241 gatggcagag acgggagaag tatgtcacga ccatattttc atattagaac ccaaaactat
     3301 caccaggaag gtaccagtga ttataaaagt gcgatgagca ttctcaaaaa acttcaggat
     3361 aaaacatcag aaaaaatcat ttttgatttc agaaatgaga tggttgagat gaaagatgaa
     3421 tcaagaccta tatatatgag cagtattgag aatactatta agtcttctca atttattata
     3481 gatgttgcgg tcggtttaac aacacaacaa ctaccagaat tgcaaaatat atgtgctgaa
     3541 ataaatcaat ccaggttaaa acatcatcgt cataatctct tcaagccgca aattgaggaa
     3601 caaaaaatca atgagcaaaa tcaatcttta tttttaggtt aaagtcaaat tcgacataag
     3661 aggcataaaa tgcttcttat gtcgctactt taacattatg ccttgttatc ttatcctgca
     3721 atatttttgt tcaactggca ttttggcatt ttaacaaacc ttcaagatca gctagagctt
     3781 tttcgtcatg gatagttaat agtgtgaccc aagaggcgat gatctgattg taggtaaaaa
     3841 tccgctttaa ccactgtttt ttcttttctt cttcagcgat gtgctcggtt aaggaaagct
     3901 gctttttgtt tagtttttcc caatttttta ttgcgttcgc cagaggatct ttattatggg
     3961 ctcttgccca ttcgtaaata atcgcgttgc gatttttttg atagtccact aaatcaggat
     4021 cgttctgttt ttctgataga tattgcaaat aatacatttc aaactgataa gggtgttggt
     4081 ataaattaag tttaacaata gtttggtaaa attctttttc aaatgtttgt tgcttctcat
     4141 tgagaggctg gctttttgat tgattcactg ctttttccca ggatggcatg atgacgttga
     4201 gtgtcagatt catcattccc tcccaaacat cttgtaattc gttgccctgt tgctctaatt
     4261 gcttttccag cgaactgatt ttttgttttt gttttgttaa ggataaggaa gcaaattcac
     4321 gggcttcggg cattaactgg actattttat cttcagaagc acgggcaaca tgtataaatt
     4381 ggtaaagggc atcattattg ctggacggaa gtcttttctt ataattcagg taaatggatt
     4441 gaatcatatt gttgtcgtaa tgtttcgtgc gggacttaaa ctgttttgat gcaaataatt
     4501 tggcccattg gagtaaataa tagtccgaag tacttaaagg aatgtctact gtcatgtctt
     4561 caatgatcgc gttttgatgg gcaaccaaca tacttttagc cggttccctg attttttttc
     4621 catcaatgac gccatgccct gtgtaccatt cggtaaaaga acgttcgtat ccttgataag
     4681 tagcgccgtt ggcatcagca gtgcagccac tggcatagtg atcatctgtt gaggaagtga
     4741 aaccacaaat tttagtgtta gctacaggat agccatcttg tgttgtaaca gataattgat
     4801 gaaacccgcc tgagtaacac tgagacatcg caaaaataac gtgttggctt ggtattaagg
     4861 tgagcagcga cgataattca tttttggata aacaggcttt gctaaaattt ttggcgttag
     4921 tgaagttgtt aataaatggt ttttgataat gccagagatc aatgcaattg ttactgtagg
     4981 gattggtatt tttatcctct aaaaacccat gaggcatgcc atgatcactg acaaagagaa
     5041 acaaattatc accagggttg atttgttgaa ttttggaaga taaaaaaaag gcatccacat
     5101 tgtatttatt ggcttgttga ttgtttgcga taatacctgt aaacatgacg tcttctttgt
     5161 tttgttgatt atcttttgta ttaatgagtt tgtgcacatc aaatggagaa ggttgtgtgg
     5221 tcggtgtgtt acctcctcca aaatagatgc ttacggtttc gggattaaat cgggtgatta
     5281 aatgctcata aagagttttt gtttgcaggt attgggtgta gtggttaagg cctgggttat
     5341 ctcctcctga cagtaccaaa acttgctggc cagcataagt agaagtggcg tgcagagcaa
     5401 ttaaaatgct gagaattatt ttcatcttca ttccatgaat cgttgtttta attcttttca
     5461 ttgttgtgtc aaggatagca attcttgtat tgaactgcta acgataattg aaacgttacg
     5521 attaaagact aagactaatt agagataggc aagatatagt cagccatcag cactgctact
     5581 ccttgttttt tgattaccct tctctaacct tactccctgt tcgagttcaa tacaggtgtt
     5641 aaataagtct atttttaata aagacattca ttttgagtaa ttgtagggca tttattgtgc
     5701 gtatggttta aaattatatc aaggaccatt attagttgat attcctgtta tgagcacatc
     5761 cgaaaaagac gttcgtgaac aaaaagtaaa aacagtcaca ttaagttttc tgggtacagg
     5821 acaacatcgt gaaaaggttc atcatattct cacgagtttt cataatacaa tctcggaagt
     5881 aaataaagac aatcctacag tggcaatgag aatgtttgat ggccctggtt cagaaccaaa
     5941 atcaggcgac agcaaggatc caattcctgg gacttatatt tataacccaa aagataattc
     6001 caagatatta attagtcctg taatctccca aacgattacc aatgccatac aaaaactgac
     6061 aggaaatttg gcgggagaag gtatagagca tttattattt gaggcagtac tttaccttaa
     6121 tgacatcatt gaaaaaaatg gaggcaaact gcctgacaca gttaatttgc atgggtttag
     6181 ccgaggggct gatacttgta tgcgcatggc gaatcttctt tatcaattat atcccgacat
     6241 aaaggtcaat ttatttctta ttgatcaggt tcccggacct gggaaacggg atgatcctca
     6301 ttcttatact gttcctccta atgtagagca ttttgagtcg acattgatgt tacatgaata
     6361 caggcctgga tttgaccctc aacattctgg aaggtatgtg attgcagatc ccgaaaaaac
     6421 aaaggtagtt gttaaaccgt attatggaga acacaatacc ggtaatcgtg ttaccgaaga
     6481 tcccaacaca aatcacaccg ctattttatt acatgatgat atgaatcgct tttgcaggga
     6541 aaccggctca ctcccttctg ttgggatttc tccacccatt attgccagag taggagataa
     6601 aaaagaggag gttagaactc attcagaatt atctccagaa aaacgctttg agttgttatg
     6661 tggtatgaag gaaaacgaat ggggctatgc caaactgacc aaaaaatatc atgagcgcag
     6721 tattctgtcg aagcgggaag attacgtaca agactccaga ttgtttgtca accaggaaca
     6781 ccgggaattg tttaaacaat tgtatcccaa atcatttaat tggttttttg aaagaaatca
     6841 tggcggtcaa acaaaaaagg aagaagtcat cactgaatta aattcactat ctgaagatcc
     6901 aaggtatgag catttttttt catcattagc gaaacacttt caaattaatg agaataatat
     6961 tgcaggaaca ttgccagaac cctcgggtat tgatagggat gaaaaaagaa gctttggaca
     7021 acccccagtc agggacaggc tttcttattt acaacattcg ctgacctcca ttgcgaatta
     7081 ttatcactat cattgtgatg agaagagctc aactaatgaa tcagttaaaa atttgctgtt
     7141 agaacgtgta aaagagtcaa ggacaaaacc ggatagcgaa gcaatcaaac atttggaaca
     7201 gaccatggac gaggtaagac agacactcga atccaaaaat gaaaaaggtt tcctctggca
     7261 acagattaat catatttctc ccaatgctcg acaatattgt gaacaagtga aagcagcact
     7321 ccgggaacac ctcgagcata atcaggtact atcagatact caaaaagagg aaattcgtaa
     7381 ggctatggat agaatggata atatagtcaa tgatggtagc aaagacagtc aacaaaaata
     7441 tagggaaata agaagagagg tgattgagtt aaatgccaag gccaccacac cggaagatga
     7501 taatcaatta acaagaagtc atttccaaaa agcctatttt gaattatcag gggatacaca
     7561 aaaaactctg aatttagaat ctctgtctca aacattgaat caactatcga aagcccatta
     7621 tggtgaaacc aatatgacgg acaaaattac tcagcgattg gatgggtata aaaatcgaaa
     7681 ttggttttgg aactcagtca gggaagtttt gaattttttc aatattccta ttcccaaatt
     7741 gcattcggag gttaaagagc aaattgctga caagctcaaa gaaagacttg ttgatctcaa
     7801 ggaaaaaggt atgggcaatg atgtgaatgc aatcaccaga gagcttggta aagctcgcga
     7861 agatttaatt gaacattata agaaaacttc aaaactggaa atgggagaac tggataagat
     7921 tatcaataaa tcaatggaag aactccttgt ggcacgcaaa gtcaccaaag acttggtcca
     7981 cgaggaagtt agtcaggtta aattaaatta aattcggttc catttttaaa agctatagtg
     8041 cgctaagttt ctggtttgtt taaagcaaaa agtacttttc tatttcgata tcatattgtc
     8101 ttttatttgg attctatgat atgttgttgt cctttcattg cctttgtaca tacccaaaaa
     8161 aatttatttg gaaaaaatat tgtttgatca ataaaatcta tcactggagt taaggttgat
     8221 ttatgttcac tttaaaaaaa aacaactcaa atatagtaag taatctcctc aacaccaatc
     8281 ttggcagttt tacaagagtt cctaatgaaa tactcttgca tgttatgtat ttccttgatc
     8341 ttcaatcact cagccgtttt gcaagaacta ataaagatat taattatcaa gtacatgagt
     8401 taagtaaagc gtttaatgtc actttaattg atataggctg cgagggaact tatagtgaat
     8461 tacatcaaaa atattcaaaa ttcctagtgg cctctcaaaa acgtcaaaag gaattacaat
     8521 caatcaaaga agttagagag tcaataaatc aaaaaagaaa agacctggaa gacaatcgag
     8581 caaacaaaag cagttgtatt tccaaagaag aaaaaacgtt tacggttgtt tgttgtgtat
     8641 taggaactgt tggtggttca ttattcgctt gctgttttcc cgtaaattgg ggtttatctg
     8701 cacttattgg aggagtatca acaaccccaa tccccttgat agcatcgcga acaacaaaat
     8761 gtgtgtgtca aaaatgtgtt gagtgtaaag cccattcact cgaattgaga gaacagaatt
     8821 tagataacaa cttcccacca ccttctaaaa tgctaaagta atgcggtgga gctgactact
     8881 ccttttgcaa taaaactgaa aggagctgag ctttgacttt ctcagatgtt aactgccttt
     8941 tttccagtta caaatctttg ctttttacaa atccctcatg accttaggaa gagaggattt
     9001 gtgaaaaaaa acaaggtcaa ggcaaaaaat gtttttaatg atgagactgg ggttttgtgt
     9061 aagtcctgga atttttagat ttttagctgc ctttctactc tgacagaatt cattatttcc
     9121 tcaattgcag ggttggcgct taaagcagct cggatttttt ggaaatcgca acagcttggt
     9181 ttggcaaatg ctttaatgac gtcatgagca ttctgattgt aattattgtc tttgttttca
     9241 gctatttttc ttttgatttc aataatggca ttggctatat tttcttcttt tgaagggtca
     9301 atcttgccta ccatatctcg tatatccttg ataatggttg gaactgaacc atcttgattt
     9361 ctaagtaatt catcattctt taataccgtg tagaccgcca aaatattttc ttctctttca
     9421 ttgacaggta tcttcatgat actaatatgt acttttgttt tatcgggtaa agtgaggatt
     9481 tcttgtctgg gatctgtgtc atgtttagtt aaaatatttt caaacttagg taggactatt
     9541 ctttctggtt cctcttgttc tgttttagca atttgttttg cagcatgctc ccaagtagaa
     9601 atggtttttc cgtcaatatt ctcggcattt tcaagtgcag cggtcttgat gaagtgtaac
     9661 gcgttatt
//
LOCUS       DAXLDT010000111         9588 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXLDT010000111 DAXLDT010000000
VERSION     DAXLDT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.111"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1103
                     /locus_tag="ACF2SP_002211"
     CDS             <1..1103
                     /locus_tag="ACF2SP_002211"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFD2359987.1"
                     /translation="ILGHCHPKVIEAVDNVLHSGMSFGAPTELEIQLAEKIASLMPSI
                     EKIRMVNSGTEATMTAIRLARGFTNKNKFIKFNGCYHGHSDSLLVKAGSGLLTLGIPS
                     TPGIPKSITEHTLTADFNNLEQVAQLFEKYPNDIATVILEPVPGNMGFILPKIEFLKG
                     LRELCDQYNALLIFDEVMTGFRVGLHGAQGLFGIKPDITTLGKIIGGGMPVGALGGKR
                     EIMSFLAPEGPVYQAGTLSGNPLAMAAGLATLKEIEKINFFEDLSNTTNKLTEALADA
                     AENANIPFFAASLGGMFGFCFTDKNSVENYFDVASSDEVLFKKFFHAMLAQGVYFAPS
                     MYEAGFVSSMHGDLEIQKTYDAAELVLNQLSA"
     gene            1502..3028
                     /locus_tag="ACF2SP_002212"
     CDS             1502..3028
                     /locus_tag="ACF2SP_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFD2359988.1"
                     /translation="MLILSKRRGLWSSMPQGTFALFFIQVVSTLSFSVLYSTLVLYMK
                     GKLGLPIATANSIMGVFIAYNFALHLLGGFWGGRLLSNRSLFCVGMIAQIVGCVLLSI
                     GNTTFLYYGLAAFLTGSGLNVTCVNCMLTQQFAPEDSRRETAFLWNYAGMNIGFFIGF
                     SLSGAFQISQNYQRLFLLSSLGNLVALLICLYFWQQLHDKNTTYSRKDKPAQRKATAL
                     GIGLIFVLPFILRQLLQYADWANKLVIVTGIIMLILILILAQQQSTKEARDKIYGFAV
                     LMLVGTVFWMLYQIGPMGLTVFIDHNVQRQYGNWIIPPQWFQNINTVVIVFGGPLLGF
                     ILNRMRQNGSQINIPTQFAFALFFIGVAFAILPIGINYANQHGMVNPGWVVISFILQS
                     IGELLISPIGYAMIGALAPTSLQGVMMGMWMLNTGVGATLSSYSSNLMIIGQESTSPL
                     LTNEGYSHVFLMLGLFAIGSSVILFILVPKLRKLIKEKNLNGFKQSGLEVTERVALCE
                     "
     gene            3021..5021
                     /gene="mnmC"
                     /locus_tag="ACF2SP_002213"
     CDS             3021..5021
                     /gene="mnmC"
                     /locus_tag="ACF2SP_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFD2359989.1"
                     /translation="MSSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFI
                     EGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKH
                     PLKKNDLIKCLQKWPELSVQTEKLIEHYPVLTPGYHHLAFNNNQITLTLMLGDVLECY
                     EQLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATY
                     SASSIVKTALTNAGFVIEKRKGFGPKRHMICAHYEKAYSSPKKNRHTPWHINYPVTKD
                     ERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQAVLFPKLSTYKSP
                     FTQFMLYSFLYANDVYKELLKHYDLGELKGSLLLAHNEREKANQQSLIHWLELYPELG
                     QLVDEKQSSELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYN
                     QKNWVVKDIEASVLILANGQQVNYFHETNHLPVKAIRGQMTTIQSTQESTQLKIPLCA
                     EGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIMWSQNVLDHWAG
                     IRAASPDYLPIVGPLPNALEFKEIYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLT
                     TIPLATEWLAGLINKEISILPRKLIQAISPSRFLRKKIIQGP"
     gene            5107..5892
                     /locus_tag="ACF2SP_002214"
     CDS             5107..5892
                     /locus_tag="ACF2SP_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFD2359990.1"
                     /translation="MQNIEFYQIDAFTDKVFHGNPAAVCILDDWLNDELLKAIAAQNN
                     LAETAFIIKDDQDFHIRWFTPNGEINLSGHATLAAGFALLESNKGPSDSIIFHHSSGQ
                     ISVEKRKEKFVLNFPRIGYKKTLSPDPIVSLLDQPAIEVFETDLDYLVLLEHESQIRN
                     LQIDLLALAKLPKRGIIVTSTTTEADFYSRCFYPKHSIAEDPVTGSAHCILAPFWAEK
                     LNKNSLYAIQGLHRKGELYCELQRNRVLISGHCRWYLRGHLVI"
     gene            6108..7226
                     /gene="prpC"
                     /locus_tag="ACF2SP_002215"
     CDS             6108..7226
                     /gene="prpC"
                     /locus_tag="ACF2SP_002215"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFD2359991.1"
                     /translation="MSSKSGGLAGVVAGQSAIATVGLAGKGLNYRGYSINDLAEYASF
                     EEVAYLLHYGELPTQKELNVYIKKLVNLRHMPDVLKTVLKLIPKNTHPMDVLRTACSF
                     LGTIEPEENFKQQYEIADRLLALFPGIMCYWYTYHFKNKEISGLSEEDTTGGHFLALL
                     HGRKPSKIEADMMNVSLILYAEHEFNASTFAARVTAATLADFYSAITSAIGTLRGPLH
                     GGANEAAMELIERFKTPDEAAIGIKQMLASKELIMGFGHRVYTTCDPRSDIIKKWSFR
                     LGEEKGNLLLYHISERIEEIMWQEKKLFPNLDFYSASAYHYCNIPTHLFTPIFVMSRI
                     TGWSAHVFEQRANNKLIRPTSEYIGPDSRPFPAIETRG"
     gene            7228..8676
                     /locus_tag="ACF2SP_002216"
     CDS             7228..8676
                     /locus_tag="ACF2SP_002216"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HFD2359992.1"
                     /translation="MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMD
                     TLGCGILALNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDF
                     NDTWLAAEWGHPSDNLGAILAVADYLCRKNCNHGRPPILMHDVLTAMIKAHEIQGCLA
                     LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNA
                     GSRKSWAAGDATSRAVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGS
                     YVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDISRIELITHESAIRIISKQG
                     ALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDSLRAKMHVTENEQFSRD
                     YHDPEKRSIANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFS
                     TDMVEKIIHAMSDTNTLSSMKVSDFMAMWMVN"
     gene            complement(8796..>9588)
                     /locus_tag="ACF2SP_002217"
     CDS             complement(8796..>9588)
                     /locus_tag="ACF2SP_002217"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFD2359993.1"
                     /translation="SDGTGITAETLGNSLITQFENIQFEKITIPYIDSTHRAESVVLR
                     INQCFSEQGTKPLVFMTLVDPEIRQAIKKAHACVFDLFSIFIGPLENELEEKSSYTVG
                     RTHGVANVKSYSHRIEAIDFALSHDDGIKTRGYDKADIILIGVSRCGKTPSCLYMALQ
                     YGILAANYPFTEEDLVGFRLPEVLRPYKPKLFGLTIDAQRLQQIRSERRPNSKYASAE
                     QCRLEVTEVEAMYQRENIPYINSTKYSIEEISTKVLAIAGLQRKI"
BASE COUNT         3011 a         1714 c         1901 g         2962 t
ORIGIN      
        1 ttatactcgg acactgccat cccaaggtga tagaagctgt ggataatgta ttacacagtg
       61 gtatgagttt tggtgctcca acagaactgg aaatacaatt agctgaaaaa attgcttccc
      121 taatgccatc aatagaaaaa attcgcatgg tgaactcagg aactgaagca actatgactg
      181 caattcggct ggcgcgcgga ttcaccaata aaaataaatt tattaaattt aacggctgtt
      241 atcatgggca tagcgatagc ctattagtta aagcgggatc cggtttgctt actctcggaa
      301 ttccttcaac tccaggcatt ccaaaaagca ttaccgaaca tactttaacg gctgatttta
      361 ataacctgga acaagttgct caactctttg aaaaatatcc aaacgacatc gctactgtaa
      421 ttttggaacc tgttcctggc aatatgggat ttatcctacc taagatagag tttttaaaag
      481 gattacgtga actttgcgat caatataatg ctttgttaat ttttgatgaa gtaatgactg
      541 gatttagagt tggtttacat ggtgcacagg gcttgtttgg gattaaacct gatatcacaa
      601 cacttggaaa aatcataggt ggtggtatgc ctgtaggtgc tttaggtggt aaacgtgaaa
      661 ttatgtcctt tctggcgcct gaaggccctg tatatcaggc tggtactctt tcaggaaatc
      721 cattggcaat ggccgcaggt ttggctactt taaaagagat tgaaaaaatt aatttttttg
      781 aagatttaag caatacaact aataaattaa ccgaagcatt agctgatgcg gcagaaaacg
      841 caaacattcc attttttgct gcatcattgg gcggaatgtt cgggttttgt tttacagata
      901 agaatagtgt tgaaaattat tttgatgttg cttcctcaga tgaagtttta tttaagaaat
      961 tttttcatgc tatgcttgct caaggagtat attttgcgcc atctatgtat gaagctggat
     1021 ttgtctccag tatgcatggt gatttggaaa tacaaaaaac atatgatgct gctgaattgg
     1081 tactgaatca attatctgcc tgatagcaga attctacctt acttaaatac aatctttaaa
     1141 atcagtattt gggtaattaa gttcagtaaa tcgcctaaat gtgattgcag aacagatttt
     1201 taaactggtt aactgcaatt ttaaataatt aataaaacac tagacagctg tttttcccta
     1261 tgtcatattc cattctctag attgcaagta tggaatcttg gtgaaaaatc agatttgcgc
     1321 ggaataagta aaaagtttct aattggacct aataacacaa ctgcagtttg attattaaaa
     1381 tatatttaat tcattttaga tactttttca cttgctgacg aaaggtggtt tttctctgat
     1441 ttgtccacaa gggttagtgt taatattcaa gaagagctca actagcaagg gaaagcatat
     1501 aatgttgatt ttgagtaaaa gaagaggact atggagttcc atgccccaag gtacatttgc
     1561 attatttttt atacaagtag tttctacttt aagctttagt gttctttatt caactcttgt
     1621 attgtacatg aaaggtaaac ttggtttacc catagccaca gcaaacagca tcatgggagt
     1681 atttatagcc tacaattttg cgctgcatct gttaggtggt ttttggggcg gcagactgct
     1741 atctaaccgc tccttatttt gtgttggaat gatcgcgcaa attgtaggat gtgttttgct
     1801 ctctatagga aatacaactt ttctatatta tggcttggct gcattcttaa caggttcagg
     1861 gttaaatgta acatgtgtaa attgtatgct gactcagcaa tttgcacctg aagactctcg
     1921 aagagaaacc gcatttctct ggaattatgc gggaatgaat attggctttt ttattggatt
     1981 tagcctcagt ggggcgttcc agatttctca aaactatcaa aggctttttc ttttaagcag
     2041 tttaggtaat cttgttgctt tgcttatatg cctttatttc tggcaacaat tacatgataa
     2101 aaacactact tactcaagaa aggacaaacc tgcacaaaga aaagcaacgg cattggggat
     2161 tgggctaatt tttgtacttc catttatttt aagacaatta ttgcagtatg ctgattgggc
     2221 caataaattg gttattgtca ctggaattat tatgctcata ttaatcttga tattagccca
     2281 acaacaatct acaaaagaag caagagacaa gatttatgga tttgcagtat taatgctggt
     2341 tggtactgta ttttggatgt tatatcaaat tggcccaatg gggttaaccg tattcattga
     2401 ccataatgtg caacgacaat atggtaattg gattattccg ccacagtggt ttcaaaatat
     2461 taatactgta gtcatagtat tcggagggcc actattgggt ttcatcttaa ataggatgcg
     2521 tcaaaatgga tctcagataa atattcctac tcaatttgct ttcgcattat tttttatagg
     2581 ggttgctttt gctatcttac caataggcat caattacgct aatcagcatg gtatggttaa
     2641 tccgggttgg gtagtaataa gctttatatt acaaagtatt ggcgaacttc taatatctcc
     2701 aattggctat gcgatgatag gagcgttggc cccaacctca ctccaggggg tcatgatggg
     2761 aatgtggatg ctgaatactg gagttggtgc cactctatcc agttatagct caaacttaat
     2821 gattattggt caagaaagta cttctccttt attaaccaat gagggttaca gtcatgtctt
     2881 tttgatgctt ggtttgtttg caataggatc atccgttatc cttttcattt tagtgcctaa
     2941 actaagaaag ctgatcaaag aaaaaaactt gaatggcttt aaacagtctg gcttagaggt
     3001 aactgaaaga gtagctttat gtgagtagtc cctttgtccc aattataacg gcagatattg
     3061 attggcgtga tgatctgccg tattccttac aatttgatga tatttactat tctgctgaag
     3121 gtggaattaa tcaaagtctt tatgttttta tcgaaggtaa taatttgatt aatcgttggc
     3181 aacaattacc aaccaatgaa tcaaacgtat ttacaatcgc tgaaactggt ttcggtacag
     3241 gtatgaattt cttattaacc tggaaactat gggaaaaatt tgcacctcaa aatgcccgct
     3301 tacattatat ttcctgtgat aaacatccat taaaaaaaaa tgacctaatc aaatgcttac
     3361 aaaagtggcc agaactgagt gtgcagactg aaaaattaat agaacactat cctgttttga
     3421 cacctggtta tcatcattta gcatttaata acaatcagat tactttaaca ttaatgctag
     3481 gagacgtgct ggaatgctat gagcaattat tattctgtgg ggacattaac cttgaacatc
     3541 aattgagaga atcctatgtt aatgcttggt acctggatgg attttcacca tcaaaaaacc
     3601 aatccatgtg gtctgacaat ctgttcactg tcattgccat gctatctaaa gaaagcacca
     3661 cagtagctac ctattctgct tccagtatcg tcaaaactgc tttaaccaat gcaggatttg
     3721 ttattgaaaa aagaaaggga tttgggccca aacgacacat gatatgcgcc cattatgaaa
     3781 aagcttattc aagtccaaaa aaaaatcgcc atacaccgtg gcacataaac tatcctgtga
     3841 caaaggacga aagaacagcg ctcattgttg gaggagggct tgcaggttgt tttattgcaa
     3901 atagcctcgc taagcgtgga tgggaagtga ccatattaga agaaaaagag aaagtgggat
     3961 gtggtggatc agcaaatcaa caggctgtat tgttccctaa gttatccacc tataaatcac
     4021 catttactca atttatgctt tattcgtttc tttatgctaa tgacgtttac aaggaacttt
     4081 tgaaacatta tgaccttggt gaattaaaag gctctttgtt gttagcgcat aacgagaggg
     4141 aaaaagcaaa ccagcaaagt cttattcatt ggctagaatt ataccctgaa ttaggacaat
     4201 tagtggatga aaagcaatct tctgaattat cagggatatc cttgccatgt ggtggtttat
     4261 ttattccatc ttctggctgg attaattccc ctgaactatg cgatattcta attgataata
     4321 aacgaatttc cctaatcact ggcaatagag ttcaatctat taactataat caaaaaaact
     4381 gggtagttaa ggacatcgaa gcctcggtac taattttggc aaatggacag caagtaaatt
     4441 attttcacga aaccaatcat ttgcctgtaa aagccattag aggacaaatg actacgattc
     4501 aatcaacaca agaaagtacc caattgaaga ttcctttatg tgccgaaggg catgttttac
     4561 cagctttaaa caatagtcat agagtggggg caagctatga tattggcaca tctgaacctg
     4621 aacttaatgc tctggatgat caattaaacc ttgaccgatt aaaacggata gcgcctgata
     4681 taatgtggtc ccaaaacgta ttagaccatt gggctggtat acgagccgca tcaccagatt
     4741 acttgcctat agtgggacct ctccccaatg cgcttgaatt taaagaaatt tattcagaat
     4801 taaaatctaa ctccaaacgg tggatagctg aagcagctcc ttgttatcca aatctatatg
     4861 tgtgtgcggc ttttgggtca agaggattaa caacaatacc tctcgcaact gaatggttag
     4921 ctggtttaat taataaggaa atttcaatac tgcccagaaa actgatacaa gctatatcgc
     4981 cctcacgatt cttaagaaaa aaaattatac agggaccata agagatgtat tgaagatata
     5041 ttgataattc gacttaactt catctttctt tatcagttat aattttaatt tttatgtgag
     5101 ctgattgtgc agaatattga gttttatcaa atagatgctt ttacggacaa agtcttccat
     5161 ggtaatccgg ctgcggtttg tattctcgat gattggttaa atgatgagct attaaaagcc
     5221 attgctgcgc aaaataattt ggcagaaacc gcttttatta taaaagatga tcaagatttt
     5281 catattcgtt ggtttactcc taatggggaa atcaatctat caggacatgc aactttggca
     5341 gcagggtttg ctttacttga gtcaaataaa ggacctagtg attctattat ttttcaccac
     5401 tccagtggac agatttcggt agaaaagaga aaggaaaagt ttgttctgaa ttttccaaga
     5461 ataggttata agaaaactct ttcaccagac ccaattgtat cactattgga tcaacctgct
     5521 atagaagtat ttgaaacaga ccttgattat ctagttttgc ttgagcatga gtcacagatt
     5581 cgaaatttgc aaatcgattt gcttgcttta gcaaaactcc ccaaaagagg aatcattgtg
     5641 acctcaacaa caacggaagc cgatttctat tcccgatgtt tttatccaaa acacagcatt
     5701 gcagaagatc ctgttaccgg ctcagcacat tgcatattag ctcctttttg ggcagaaaaa
     5761 ctcaataaaa acagcctata tgctatccag ggattacaca gaaaagggga gttatattgt
     5821 gagctccaga ggaatagggt attgatttcc ggccattgtc gatggtatct acgcggacat
     5881 ttagtaattt aataaaatcg attgagatgc attcatcatt gaagcataat ataaaaccca
     5941 tttaggagcc aatggagcat gataacaata tctattccaa aaggaattct ctccaagaga
     6001 ataaggtaaa ataatcaacc gcttcaaagt tcagtaataa taaaaacata tgcagcaatt
     6061 ttgactgcac tagaaataaa aactagacca actgatggag gaagaaaatg agtagcaaaa
     6121 gtggaggttt agccggtgta gttgctggtc aatcagcaat tgccacagtt ggactggcag
     6181 gcaaaggatt aaattacaga ggctattcaa taaatgatct agctgaatat gcaagttttg
     6241 aggaagttgc ttatctgctt cattatggtg aacttcctac ccaaaaggaa cttaatgtat
     6301 atataaaaaa gctggttaac ctaagacata tgcctgatgt gcttaaaacg gttttgaaac
     6361 ttatacctaa aaacactcat ccaatggatg tactgcgaac agcatgttct tttttaggca
     6421 ctatcgaacc tgaagagaat tttaagcaac aatatgaaat agctgatcgt ttgctagctc
     6481 ttttcccagg aataatgtgt tattggtata catatcattt caaaaataaa gaaatctctg
     6541 gcttaagtga agaagacaca acaggaggac actttctggc gttattacac ggacgcaagc
     6601 ccagcaaaat agaagccgat atgatgaatg tctcattgat cttgtacgct gaacacgaat
     6661 ttaatgcttc tacctttgct gcaagagtta cagcggcaac actggctgat ttttactcag
     6721 cgatcacaag tgctatagga acattgcgtg gtcctttgca tggtggcgcc aatgaagcag
     6781 ctatggagtt aattgagcgc tttaaaaccc ctgatgaagc agcgataggc ataaagcaaa
     6841 tgcttgccag caaagaactg atcatgggat ttggacatcg ggtttataca acttgtgatc
     6901 cacgctcaga tattattaag aaatggtcct ttagattagg tgaagaaaag gggaacctac
     6961 ttttatacca tatttcagaa cgtattgaag aaatcatgtg gcaagagaaa aaactatttc
     7021 ccaacctgga tttttatagt gcatccgctt atcattactg caatatacca actcatttgt
     7081 ttaccccaat ttttgtgatg tcacgcatca caggatggtc tgcgcacgta tttgagcaaa
     7141 gagcaaataa taaactaata agacctactt ccgaatatat aggcccagat tccaggccat
     7201 ttcctgccat tgaaacacga ggttaatatg cacaattatg tagaggataa tgttaagcca
     7261 gactatgatc aggttataac agatatagct gattttgtat taaataaaga aattgatagt
     7321 gtgcaggcct atgagactgc acgattatgc ttaatggata cactcggttg tggcatattg
     7381 gcccttaatt ttcaggaatg taccaaatta atgggaccta tagtaccggg agcaactctt
     7441 cctggaggcg cgagagtacc aggtactaat tatgaactgg atccggttca agctgccttt
     7501 aacatcggta cgatgatacg ctggctagac tttaatgata cttggctcgc tgcagaatgg
     7561 gggcacccct ctgataattt gggtgctatt ttggctgttg ccgattattt gtgccggaaa
     7621 aattgtaatc atggacgtcc tcccattttg atgcatgatg tacttacggc aatgattaaa
     7681 gcacatgaaa ttcaaggttg tttggccctg gaaaatagct tcaatcgcgt cggccttgat
     7741 catgtcatat tagttaaagt agcgagtgcc gctgttgctg ctcacttgtt tggagcagat
     7801 cgtgatatga tgctaagaac tctctcgcag gtttttgtgg atggacaaag cttgagaaca
     7861 tatagacatg ctcccaatgc aggttcaagg aaatcatggg cagcaggaga tgctacctcc
     7921 cgtgcggttc gtttggcttt aattgccaag gcaggggaaa tgggatatcc aagcgccttg
     7981 accgcacaaa catggggatt ttatgacgtg ctatttggta aaaaacaatt caagtttcaa
     8041 cgcccttacg gcagctatgt gatggaaaat gtgttattca agttatctta tcctgctgaa
     8101 tttcatgcac aaaccgctgt cgaatgtgca gtaaaattac atcctatagt aaaaaatagg
     8161 ttagaggaca tttctagaat tgaattaatc acacatgagt cagcaataag aataataagt
     8221 aagcaaggtg ctttatataa tcctgccgac agagatcact gcttgcaata tatggttgca
     8281 gtaggacttc tctttggtga tttaaaggca gagcattatg aagacgacgt tgccgcagat
     8341 cccagaatag attctttacg agctaaaatg catgtaactg aaaatgaaca gttttcacgc
     8401 gattatcatg atccggaaaa acgctctata gctaacagca tgcgaatcat ctttaaagat
     8461 ggctcagaaa gtgaattaat aactatagaa taccctattg gtcataaacg gagacgagaa
     8521 gaggggattc ccgttttaat gtctaaattc aagcgtaatc tgagtacaag attctctact
     8581 gacatggttg aaaagattat tcatgccatg agcgacacca acacgctatc atctatgaaa
     8641 gtaagtgatt ttatggccat gtggatggta aattaaaaag actaacaccc acgctcaatc
     8701 tgttatcttt actgatttta gcacagacca ggagggggct aagcgtatag caaagccaac
     8761 cctccttact aaatcgtagc cttcaaaaat aggttttata ttttcctttg taacccggca
     8821 atagcaagaa cctttgtcga tatttcttct atggaatatt ttgttgaatt gatataagga
     8881 atattttctc gttggtacat ggcttctact tctgttacct ctaatctgca ctgctctgcg
     8941 gaggcatatt tgctatttgg tctacgttca gagcgaattt gctgcaaacg ttgtgcgtca
     9001 attgttagtc caaataattt tggtttataa ggtcttaata cctctggtaa gcgaaaaccc
     9061 accaaatctt cttctgtaaa aggataattg gcagctaaaa tgccatattg cagtgccata
     9121 tataagcaac tgggagtttt gccacaacgt gaaactccta taagaataat atctgcttta
     9181 tcgtaaccac gcgtttttat tccatcgtca tgtgaaaggg caaaatcaat tgcttcaatt
     9241 cgatgggaat aggatttcac attcgcaacc ccatgagtcc tgcctactgt ataagatgat
     9301 ttctcttcaa gctcattttc caatggccca ataaaaatac taaacaaatc aaaaacacag
     9361 gcatgcgcct ttttaatggc ttgtctaatt tctggatcaa ctaaagtcat gaatactaag
     9421 ggcttggttc cttgctctga aaaacattga ttaattctta gtacaacact ttctgctctg
     9481 tgagttgaat ctatgtaggg tattgttatt ttttcaaatt gaatattctc aaattgagta
     9541 atcagactat tacctaatgt ctcagccgta attcctgtac catcggaa
//
LOCUS       DAXLDT010000112         9497 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXLDT010000112 DAXLDT010000000
VERSION     DAXLDT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.112"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(139..906)
                     /gene="pcsA"
                     /locus_tag="ACF2SP_002218"
     CDS             complement(139..906)
                     /gene="pcsA"
                     /locus_tag="ACF2SP_002218"
                     /EC_number="2.7.8.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050520 - phosphatidylcholine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine synthase"
                     /protein_id="HFD2359994.1"
                     /translation="MNPIKPPFTLNQYFAAWFVHVFTASAACIGVFSLYKIYQHDYVF
                     ALWLMAITVFIDAVDGSLARLVHVKSVLPKIDGALLDNIVDYLNYVITPCFFLLVKPG
                     MLPADYVVPIIAAITITSAYQFCQDDAKTPDHFFKGFPCYWNITVFYMYIFNTSMIVN
                     TVLLSLFCVLIFIPVKYVYPSRLDYLTESRILKILMHCCSALYGISSFCLLVNYPETN
                     KLWVSLSLGYVGMYLFLSFYRTYYPMFKAKITANNKD"
     gene            1249..2637
                     /locus_tag="ACF2SP_002219"
     CDS             1249..2637
                     /locus_tag="ACF2SP_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFD2359995.1"
                     /translation="MNIQTVNPTTEEVLNNYTCLDFDSIDKKIACGYEAYLNWKNSKF
                     DIRRSLMLNLAQILKDKKQELAHLMALEMGKPITAGRAEIEKCAWVCEHYAENAEQYL
                     MPRTVQTEMKTAKVCYRPLGIVFAIMPWNFPFWQVFRFSAPTIMAGNGAILKHAPISS
                     GTGNKIEELFRDAGFPSSLFQHLIVDNDGASHIIENQFVTAVTLTGSERAGQSVAANA
                     GKNLKKSVLELGGSDPYLILEDADLDLAAQCIVASRLNNTGQVCIAAKRVIALQSIEK
                     ELISKIKELISKYRMGDPLDPNINLGPMARKDLRENLHSQVEKSVKQGAKLLLGGIIP
                     EGKGYFYPPTLLTDVKPGMTAFDEELFGPVIVIITAKNESEAISLANMSQYGLGAAVF
                     TKDLNKGEHIATYEIEAGTCFVNAFVASDPRLPFGGIKRSGYGRELSREGILEFVNTK
                     TIAISDPKHGKK"
     gene            2624..3112
                     /locus_tag="ACF2SP_002220"
     CDS             2624..3112
                     /locus_tag="ACF2SP_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFD2359996.1"
                     /translation="MVKNSKFIFISIKKQEMSCFEKDHLIANYAISSGKNGVGEKINS
                     GCTPRGWHKIHSILGIDLPINSVMVAREWTGEIYSESLATQFPERDWILTRILQLDGL
                     EPGRNKGEDVDTLHRYIYIHGTPDTTQLGIPGSHGCIRLNNDAVIELAAWATKNTLVY
                     IQ"
     gene            3164..8041
                     /locus_tag="ACF2SP_002221"
     CDS             3164..8041
                     /locus_tag="ACF2SP_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HFD2359997.1"
                     /translation="MSYKFEEGKDAIVKSVVEKIKQTMTGEQTEFCAEFAKQFYGTVA
                     LEDLLEWEVDDLYGAAVNFWSLICERAPHETKIRIYNPDYERHGWQTTHTVVEVICED
                     MPFIVDSLRIVINRMGLTSHLTIHMGGIRVKRDSHNRVTEILPRNGGAARDDVLHEAP
                     IFIEIDRQTDPATLSELHKNFERALEDNRAVFEDWDKMRTKVREIIKELDTVPKTIDI
                     SEIEETKAFLNWMEDHHFTFLGLRDYDLIKKGKETILQPIPETGLGVLRQSLSKSNAR
                     SITAMGPEAQGLISSPRILVMSKTNTLASVHRDAYTDYIGVKRFNKKGEVIGERRIIG
                     LYTSAAYHTNPKHIPFLRHKVALIMKNSNLNPYSHAGKVLLNILETLPRDDLIQGTED
                     ELLEIAMGIFYMQDRKRIRLFARADVYKRFISCLVYVPKDRFNTELRYAMQKILADSF
                     NAEEITFSTQFSESVLARIHFIVRVNPKNLPDFDLKEIEQKLIEVGRSWIDDLQHHLH
                     EVYGEEQANALYSRYKNAFPISYSDTFSPRTAVYDIKHIEMLSPENPLGINFYKPLDE
                     SEKSFRLKVYQHDTTIPLSDVLPILEKLGLRAISERPYVLKFEDGKVAWINDFAMQYN
                     KESEFNIDEIKELFQNAFARVWFGDAENDGFNLLVLAAGLNWREVAVLRTYAKYFRQI
                     GFTFSQDYMETALNNNVSIAKKLVRLFEIRCNPHDSKRREDRYVALVAEILSDLDNVA
                     NLDEDRIIRQYVHAIGATLRTNFYQVDAQGNPKNYISIKLSSKLIPGVPKPYPMFEIF
                     VYSPRFEGVHLRCGKVARGGLRWSDRREDFRTEILGLMKAQQVKNSVIVPSGAKGGFV
                     PKQIPANATREEIMEEGISCYKLFIRGLLDITDNYKEGLIVKPQNVVFYDEDDPYLVV
                     AADKGTATFSDIANSISQEYDFWLGDAFASGGSVGYDHKKMGITAKGAWESVKRHFYE
                     LNRDIQNNDFTVVGIGDMAGDVFGNGMLLSRHIKLVGAFNHVHIFVDPNPEAEASFKE
                     RERLFNLPRSSWTDYDKKLISKGGGVFSRSAKSIPLSPEMQKVFGIKQTSIEPNDLIK
                     AILKADVDLLWSAGIGTYVKSSTESNTSVGDRTNDATRVNANQLRCKVIGEGGNLGLT
                     QLARVEYSLHGGKVYTDFIDNSGGVNCSDKEVNIKILLNNVVSAGDLTPKQRNELLSN
                     MTDEVAKLVLRDNFLQTRAISLTASQALRAIELHARYINELEKTGKIDRTLEFLPDEK
                     VLMEHKLMGKGLTQPGIAVLMCYSKTILKEQVLASEVPEEDYMNQILIGSFPKPLQER
                     FSKQMQDHPLRREIIATRLSNIIVNEMGFTYVYRLQDETGASVAAIVRAYMIARSVLN
                     LEAIWKQIEELGTKISAQAQVDMMMLYVRLSRRVTRWFLRTHRRSMSITQAIELYAKG
                     VDELKKSMPAVFGETGRIQYEEHYQEWIKEGIPPQLAHELTVTRGLFAATDIIEIAYE
                     ENIKISKVAEIYFGIGEFLDIAWLRTQIIVHTTENHWESLSREALRDDLDWQQRQLTA
                     AIMGFEPNNKDLQERLTRWGETHVSLIDRWNYILTALKSSTALNYTMFLVATRELLDL
                     TQTTLQSNLKMEESF"
     gene            8062..8610
                     /locus_tag="ACF2SP_002222"
     CDS             8062..8610
                     /locus_tag="ACF2SP_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFD2359998.1"
                     /translation="MEAKTASEYSTLSKLFHWIIALAVIVMLIAGFFLDEIPEQFQGV
                     AYMLHKSTGITILFLMILRFIWIHAKGKPALPASVKSWEKILSRFVQYGFYVLLIIMP
                     LSGWIMSVAADRIPNYFGLFKAPLPWIEPNKSLAKLMAESHEIIAWILIAFITLHVLG
                     ALKHHFIDKDNVLKRMLPGKSK"
     gene            complement(8641..>9497)
                     /locus_tag="ACF2SP_002223"
     CDS             complement(8641..>9497)
                     /locus_tag="ACF2SP_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFD2359999.1"
                     /translation="GEKSIDFLQGQLTCDLRLVSDTSMIQGAQCNLKGRILSLLDIIN
                     WQGVKLVLPQDLIEVTQNSLNKVALLSRVKITSNHNYKILGFYLQHGNDQIPQLLPLS
                     SELYALSCTSHGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNN
                     QIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQS
                     DNQLYSGQKLFKSDEDTEVGELIDYSMLGENRYLVAASILKDNFSSVCLEGNKFPIQL
                     EEKIELEL"
BASE COUNT         3210 a         1551 c         1905 g         2831 t
ORIGIN      
        1 cttgcattac ctctctattt tttcagtaaa cagatactat ttaaagcaat aagaaaagaa
       61 aaattcctaa aagagtacga taaaacagtg ccaccagaga tctaatatca gcatggaaaa
      121 cataaattgc atataaactc aatctttatt atttgccgta atcttagctt tgaacatcgg
      181 ataataagtc ctgtagaaac ttaaaaataa atacatgccc acatagccta aagataggct
      241 tacccataat ttatttgttt cagggtaatt aactagcagg caaaatgaac tgatgccata
      301 aagagcagaa caacaatgca ttaatatttt caagattctc gactcagtca agtaatctaa
      361 ccttgatggg taaacatatt ttactggaat aaaaatcagt acacaaaaaa gggacaagag
      421 gacagtgttc acaatcatag aagtgttaaa aatatacata tagaagacag taatattcca
      481 atagcagggg aaccctttaa aaaagtgatc aggggttttg gcgtcatcct ggcagaattg
      541 ataggccgaa gtaattgtaa tagcagctat aattggcacg acataatcag ctggcagcat
      601 acccggttta actagcaaga aaaaacatgg agtaatgaca taattcaaat agtcaactat
      661 attgtctaat aaagcgccat caattttagg caatacgctt tttacatgaa ccaatcgagc
      721 aagactacca tctactgcgt caataaatac agtaattgcc attagccata aagcaaatac
      781 ataatcatgc tggtaaattt tatataagct aaagacacca atacaggcag cgctggcagt
      841 aaatacatgg acaaaccagg cggcaaaata ctgattaagg gtaaagggag gctttattgg
      901 attcattact tatcctagag ttaatcaatg tcatctacta ctaattaggg cttcagtctt
      961 ttttgtaaaa gcaaaaaaca aattaccttt aaaaatatcc agtgcaatga ttaaatctgg
     1021 attgttttat tcatcaagac gatgtgtttt tactttttac taaaacagtg caagtccttt
     1081 ttacttaaca ggttgatttt tattactgtt ctttaagaca atgtcgttta aaataaaaat
     1141 agtatcaata ttcaaaagca aagtgcaagt taggttaatt atttaaaata aaaaaaagcc
     1201 ttgatttggt ctaaactata ggcaacgtta atcatatcga gtaattaaat gaatattcaa
     1261 actgtcaacc cgacaactga agaagttcta aataactaca catgcttgga ttttgattca
     1321 atagacaaaa aaatagcgtg tggatatgag gcctatttaa attggaaaaa ttctaaattc
     1381 gacataaggc gctctttaat gctcaatctt gctcagatat taaaagataa aaagcaagag
     1441 ttagcgcatt taatggcttt agaaatgggt aaacccatta cagcaggtcg tgctgaaatt
     1501 gagaagtgtg cttgggtatg tgaacattat gctgaaaatg cagaacaata cctaatgcca
     1561 agaactgttc aaactgaaat gaaaacagca aaagtttgtt acagaccact aggaattgta
     1621 tttgccataa tgccttggaa tttcccattt tggcaggttt ttagattttc agcaccaact
     1681 atcatggcgg gtaatggggc tattttaaaa catgccccaa tatcgagtgg aacaggaaat
     1741 aaaatcgaag aattattcag agacgctgga tttccttctt cattatttca acacttaatt
     1801 gtagataatg atggggcatc tcatatcatt gaaaatcaat ttgttaccgc agttacttta
     1861 actggaagtg aacgtgctgg tcaaagtgtt gcagcgaacg caggtaaaaa tttgaaaaaa
     1921 tcggttctag aattaggtgg ttcagatccc tatttgattc tggaagatgc ggatttagat
     1981 cttgctgctc aatgcattgt ggcttcacga ttaaataata ccgggcaggt ctgcattgcg
     2041 gccaagcgag ttatagcact tcaatcgatt gaaaaagaat taatttctaa aatcaaagaa
     2101 ctcatcagca aatacagaat gggagatcca ttagacccca atattaattt gggacccatg
     2161 gcaagaaaag atttacgaga aaatttgcat tcacaagtag aaaaatctgt taaacaaggt
     2221 gctaaattat tgcttggtgg tataattccg gagggtaaag ggtacttcta cccgccgaca
     2281 ttattaactg atgttaaacc tggaatgaca gcttttgatg aggaactatt tgggcctgtt
     2341 attgttatta ttactgctaa aaatgagagt gaggcaattt cactggccaa tatgagtcag
     2401 tatggtttag gtgctgctgt atttacaaag gatcttaata agggagagca tattgctact
     2461 tatgaaattg aagcaggaac ctgttttgtt aatgcttttg tagcctctga tccaagactt
     2521 ccttttgggg gaataaagcg atcaggatat ggtagagagt tatcaagaga aggtatatta
     2581 gagtttgtaa ataccaaaac aattgcgatt agtgacccaa aacatggtaa aaaatagtaa
     2641 attcattttt atttcaataa aaaaacagga gatgagttgt tttgaaaagg atcatttaat
     2701 tgcaaactat gctatttcat ccgggaaaaa tggcgtgggt gaaaaaatta atagtggatg
     2761 caccccgaga ggttggcata agatccattc tatacttggc attgatctgc caattaatag
     2821 tgtgatggtt gccagagaat ggactggtga gatttattca gaatcattgg caactcaatt
     2881 tccagaaagg gattggatat taacccgaat tttacaattg gatggattag agcctggtcg
     2941 aaacaagggc gaagatgtag atactttaca tcgttacatt tatattcacg gcacaccaga
     3001 tacaactcaa ctaggtatac ctggctcaca tggatgcatt cgcctgaata atgatgcagt
     3061 tattgagctg gcagcttggg caacaaagaa tactttagta tacatacaat gattaataac
     3121 aattaatgct tcggagcata agtgaacctg aaggggtaaa tgaatgtcct ataaatttga
     3181 agaaggtaaa gacgcaatag taaaaagcgt tgttgaaaaa attaagcaaa caatgactgg
     3241 cgaacaaaca gagttttgcg ctgaattcgc gaagcaattt tatggaactg tcgcccttga
     3301 agatcttcta gagtgggagg ttgatgatct ttatggagca gcagtcaatt tctggtcttt
     3361 gatttgtgaa cgcgctccac acgaaacaaa aatcaggatt tataatcctg attatgaacg
     3421 tcatggatgg caaactacac atactgttgt agaagttatc tgcgaagata tgccttttat
     3481 tgtagattct ctgaggatag taattaatcg aatgggatta acatctcatc ttactattca
     3541 tatgggagga attagggtta aaagagacag ccataacagg gttactgaaa ttcttccaag
     3601 aaatggtgga gctgctagag atgatgtttt gcatgaggcc cccattttta tagaaattga
     3661 tcgacaaaca gacccggcta ccttatcgga gctgcacaaa aattttgaac gcgctcttga
     3721 ggacaatcga gcggtatttg aagattggga taaaatgcgt actaaagtac gagaaattat
     3781 taaggagctt gatactgtac caaagacaat tgatatcagt gaaattgaag agacgaaagc
     3841 ctttttaaat tggatggaag atcaccattt tactttttta ggattgcgag attatgattt
     3901 gattaaaaaa ggaaaagaaa ccattttaca acctattcct gaaacaggat tgggagtttt
     3961 acgccaaagt ctgagcaaat caaatgcaag aagtattacg gcaatgggac cagaggcaca
     4021 aggattaatt tcatcacccc gcattcttgt catgtccaaa acaaatactt tagcctctgt
     4081 tcatagagat gcttataccg actatattgg tgttaagcgc ttcaataaaa aaggagaggt
     4141 gattggcgaa aggcgtatta taggactgta tacttctgca gcttatcata caaatcccaa
     4201 gcatattccg tttttgcgac ataaagtcgc attaattatg aaaaattcta atttaaatcc
     4261 ctatagccat gcaggtaaag tattattgaa tatacttgaa actcttccaa gagatgattt
     4321 aattcaaggc actgaggatg agttactgga aatagctatg ggtattttct atatgcaaga
     4381 tagaaaacgt atccgcctgt ttgccagagc cgatgtttac aagcgattta tttcatgctt
     4441 ggtatatgtt cccaaggatc ggtttaatac tgaattaaga tatgccatgc aaaaaatttt
     4501 ggcagacagt tttaacgctg aagagattac cttttctact cagttttcgg aatcggtatt
     4561 ggcaagaatt cattttatag taagggttaa tcccaaaaat ttacccgatt ttgatttaaa
     4621 agaaatagag caaaaattga ttgaagtggg gcggtcgtgg attgatgatt tgcaacatca
     4681 tttacatgaa gtctatggcg aagaacaagc caatgcactt tactctcggt ataaaaatgc
     4741 atttccaata tcttattctg ataccttttc accaagaacg gctgtctatg atattaagca
     4801 tattgaaatg ctaagccctg aaaatccact cggtattaat ttctataagc cattggatga
     4861 atctgagaaa agctttaggt taaaggttta tcagcatgac accactattc ctttgtctga
     4921 tgtactacct atcctggaaa aacttggttt aagagcaatt agtgaacgtc cttatgtttt
     4981 aaaatttgaa gatggtaaag tagcatggat taatgatttt gccatgcaat acaacaaaga
     5041 aagcgagttt aatatcgatg aaataaaaga gttatttcaa aatgcatttg ccagggtatg
     5101 gtttggtgat gcagaaaatg atggctttaa tttattggta ttagcggccg gattaaattg
     5161 gagggaagtt gctgtattaa gaacctatgc aaaatatttc aggcaaatag ggtttacatt
     5221 tagccaggat tatatggaaa cagcactaaa taataatgta agcattgcca agaaattagt
     5281 taggttattt gaaattcggt gcaatcccca tgactccaaa aggagagagg atagatacgt
     5341 tgcacttgtc gctgaaattt tatctgatct tgataatgta gctaatctgg atgaagacag
     5401 aattataaga caatacgtgc atgccatcgg cgccactctt cgtaccaatt tttatcaagt
     5461 agacgctcaa ggtaatccta agaattatat ttctataaaa ttatccagca aactcattcc
     5521 tggtgtacca aaaccatacc ccatgtttga aatttttgtt tactcgccac gatttgaagg
     5581 ggtgcatttg cgctgtggta aagtggctcg cggaggttta cgctggtcgg acagacgtga
     5641 agatttccga accgaaattc ttggcttaat gaaggctcaa caggtaaaga actctgtaat
     5701 tgtccccagt ggcgcgaaag gtggttttgt acccaaacag ataccagcta atgctactcg
     5761 tgaagaaatt atggaggagg gaatcagctg ttataaactc tttataagag gtttgttgga
     5821 tataacagac aattacaaag aaggactcat tgtaaaaccc caaaatgttg ttttttatga
     5881 tgaagatgac ccttatcttg tggtcgctgc agacaaaggc actgcgacat tttctgatat
     5941 tgccaattca atttcccagg aatatgattt ttggcttgga gatgcttttg cctcaggtgg
     6001 ctctgttggt tatgatcata aaaaaatggg gattactgct aaaggggcat gggaatctgt
     6061 taaacgccat ttctatgaat tgaacagaga tatccaaaac aatgatttca ctgtggttgg
     6121 cataggagat atggccgggg atgtctttgg aaatggcatg ttgttgtcca ggcatatcaa
     6181 actcgtaggt gcttttaatc atgttcatat atttgtagac cctaatcctg aagctgaagc
     6241 tagctttaaa gagcgtgaac gattattcaa tcttcccaga tctagctgga cagactatga
     6301 taaaaaactc atttccaaag gaggaggggt tttttctcgt agtgcaaaat ctatcccgct
     6361 tagccctgaa atgcagaaag tatttggaat aaaacaaact tcgattgaac ctaatgatct
     6421 aattaaagca atattaaaag ccgatgtgga tttactttgg agtgctggaa ttggaaccta
     6481 tgtcaaatcg agtactgaaa gtaataccag cgtaggagat cgaacaaacg atgcaactcg
     6541 tgttaatgcc aatcagttga gatgcaaagt tataggtgaa ggtgggaatt tgggattaac
     6601 ccagttagca cgagtggagt atagtttaca cggtggcaaa gtctatacag atttcataga
     6661 taattctggt ggggttaatt gctcagacaa agaagttaat attaagattt tattgaataa
     6721 tgttgtatca gctggggatc tgactccaaa acaaagaaat gagcttttaa gtaatatgac
     6781 cgatgaagta gccaaattag tgctcagaga taatttccta caaacccgtg ccattagctt
     6841 aactgcttct caagccttaa gagccattga attacatgca agatatatta acgaactgga
     6901 aaaaacagga aaaattgaca gaactttaga gtttttacct gatgaaaaag tcctaatgga
     6961 acacaaacta atggggaaag gattaaccca acctggaatt gctgttttaa tgtgctacag
     7021 caaaaccata ttaaaagagc aagttcttgc ttctgaagtt cctgaagaag attatatgaa
     7081 tcaaatactt attggttcat ttcccaaacc tctgcaagag cgttttagca aacaaatgca
     7141 agatcatccc ttaagaaggg agataattgc tacccgttta agtaacatta ttgttaatga
     7201 aatgggcttc acgtatgttt acaggttaca agatgaaact ggagcctctg ttgcggcaat
     7261 tgtcagggcc tacatgattg ccagaagtgt cttgaatctg gaagcaatat ggaaacaaat
     7321 tgaggaactt ggtacaaaaa tcagcgcgca agctcaagtg gatatgatga tgctgtatgt
     7381 tcgactttcc aggagagtga caaggtggtt tttacggaca catagaaggt cgatgagtat
     7441 aacgcaggcg atagagttat atgcaaaagg ggttgatgaa ctgaaaaaat ccatgcctgc
     7501 cgtttttggt gaaacaggcc ggattcaata tgaggagcat taccaagagt ggataaaaga
     7561 agggatacca cctcaacttg cccatgaatt aacagtgact cgaggcttgt ttgccgcaac
     7621 agacattata gaaattgcct acgaggaaaa tataaaaata tcaaaagtag ctgaaatata
     7681 ttttggtatt ggcgaatttc ttgatatagc ttggcttaga acccaaatta ttgttcacac
     7741 aactgaaaat cattgggaat ctttatctcg agaagcatta cgcgatgatt tggattggca
     7801 acaacgccag ttaacagctg ccataatggg ttttgagccg aataataagg atttgcaaga
     7861 acgattaaca agatggggag agacacacgt ttctttaatt gacaggtgga attatattct
     7921 gacagctctg aaatcaagta cagctttgaa ctatactatg tttttagttg ccacccgaga
     7981 gttgctggat ttaacccaaa cgacattaca atccaattta aaaatggaag agtcattttg
     8041 acatgaaagg agttaaatga aatggaggct aaaactgctt cagaatattc aacactgagc
     8101 aaattatttc attggatcat tgcattggca gttatagtca tgctaatagc aggttttttt
     8161 cttgatgaaa tacctgagca atttcagggg gttgcttata tgcttcataa atcaacagga
     8221 attactatcc ttttcttgat gattttgcgg tttatttgga tccatgctaa aggaaaacct
     8281 gctttaccag cgtctgttaa atcttgggag aaaatattat caaggtttgt gcaatatggt
     8341 ttttacgtgc tcttgattat tatgccattg agtggttgga tcatgtcggt tgcagcagac
     8401 agaataccaa attactttgg cttgtttaaa gcgccacttc catggattga acctaataaa
     8461 tctctagcaa aattaatggc tgaatcccat gaaattattg cgtggatttt aatcgccttc
     8521 atcacattac atgttttagg tgctcttaag catcatttta ttgataaaga caatgtattg
     8581 aagcgtatgc tgcctggaaa aagcaaatag ccttctgttt ttggaaattg agcctgaact
     8641 tcaaagttca agctcaattt tttcttctaa ttggattgga aatttattgc cttccaaaca
     8701 gacagatgaa aagttatctt ttaatatgct tgcagcaacc aaatatcgat tttcaccaag
     8761 catggagtaa tcaattaact ctccaacttc tgtgtcttca tccgatttaa ataatttttg
     8821 accagaatat aattgattat ctgactgaat tacaaatttc tttaattcat gcttaagagt
     8881 agctctatag tgagttcttg cgatgatttc ctgaccttta taacaccctt tatcaaaact
     8941 gacgtacgcg gtttgatgta agccaattct atgtggtaaa aagagtcccc tagaattagg
     9001 ataaatatca atttgattgt taaataatct aagggtatgc caggttaatg aaccaagtaa
     9061 ttgattattt tcaataaaag gcttgcacaa actatcataa tagtctgaat ggattaaata
     9121 gatatagaat cctttgccta agtgataaac acaaccatga ctcgtacaag ataaagcata
     9181 caattcgctg gacaaaggta gtaactgtgg tatttgatca ttaccatgct gtaaataaaa
     9241 gcctaaaatt ttatagttgt gatttgaagt gattttaact cgagaaagca gtgcaacttt
     9301 gtttaatgaa ttctgagtta cttcaattaa atcttggggc agaactaatt ttacaccttg
     9361 ccagttgata atatccaata aggaaagaat tctcccttta aggttacatt gggccccttg
     9421 aatcattgaa gtatccgata ccaatcgtaa atcacaagtt aactgccctt gcagaaaatc
     9481 tattgatttc tctccaa
//
LOCUS       DAXLDT010000113         9466 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXLDT010000113 DAXLDT010000000
VERSION     DAXLDT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.113"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            37..113
                     /locus_tag="ACF2SP_002224"
     tRNA            37..113
                     /locus_tag="ACF2SP_002224"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71..73,aa:Pro,seq:cgg)
     gene            145..708
                     /locus_tag="ACF2SP_002225"
     CDS             145..708
                     /locus_tag="ACF2SP_002225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360000.1"
                     /translation="MNINFEKANASYIELIFSWLKEPFVQEFWDNTQAHKDDILNFIN
                     GRTEPSSYADGKYVYWIASGNGHPFAMLMTIQETSEDEIDEIKLINLSKTGHSYGIDY
                     MIGDKNYFGIGYGARTLIEFIDFFIKEVDPKADTFLIDPESDNPRAKHVYIKAGFEYV
                     ADFLMSGDVSGAGKLHHLLIKRIGLIK"
     gene            705..1115
                     /locus_tag="ACF2SP_002226"
     CDS             705..1115
                     /locus_tag="ACF2SP_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance glutathione transferase"
                     /protein_id="HFD2360001.1"
                     /translation="MITGINHITLAIKDLNKSFAFYANVLGFKPLVRWDKGAYFLAGD
                     FWFCLNVDANQRPTPCYTHYAFTVTQEQFDEMSKAIIQSGIKIFKENTSPDDSLYFLD
                     PDGHKLEIHAGNWQSRLIAKKINPGTWQNVEWFV"
     gene            1112..2110
                     /locus_tag="ACF2SP_002227"
     CDS             1112..2110
                     /locus_tag="ACF2SP_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2360002.1"
                     /translation="MKSNHIKRLCQHFNLGDPTQEPQRVPGGLLHIMWQLNTDKGSFA
                     VKQLSKDINLTDYHVIRNYELSENIASGFIALGIPAVTAIEKSGKHLFIINETGFLVY
                     PWVNAKALDKDVVSVSQALEIARILSKMHQIDLQLKEIEEAEFDIHEKDKIIALIDLS
                     QKKKLPIASSLMEKLVTILEINTNYHDAILMLKAHSVISHGDLDQKNVLWDSSKKPIL
                     IDWESARKLNPTYEIVNAALDWSGITTQLDKNLFGKMLRAYKEAGGVIDKDIVEAAFF
                     GVLGNWINWMVYNIQRAANHCVPEQQKIGMEQVMQVLPTVLRLKSLIPELVNAGKA"
     gene            2107..2574
                     /locus_tag="ACF2SP_002228"
     CDS             2107..2574
                     /locus_tag="ACF2SP_002228"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360003.1"
                     /translation="MITIDLLKNHPQSIARCATIWHEVLGKIWVPEIPVEQVIQRFSE
                     HLNADKLPITFIALDTGKPVGMCSLRENDGIRPEISPWLGSLVVDPVYQRQGIGNKLI
                     EATKNKARDLGFNKLHLFAFDPTIPNYYSRLGWTIIGMDKFKGHPVTVMEIVL"
     gene            2571..3179
                     /locus_tag="ACF2SP_002229"
     CDS             2571..3179
                     /locus_tag="ACF2SP_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD2360004.1"
                     /translation="MTPHIIGISGITGAGKSTLAKALSLDLKATLISWDDFDDISLEP
                     EDYIEWYHKGCNYSEFQREDLAKVLAELKAKREIMHPVLGTLLNPAEYIIFDAPLGKL
                     HVQTGEYIDTCVHIEVPLDISLCRRILRDFKDKLQTKENLLEEISFYLNHSRPLFFDD
                     ELKQSATIVIDGLLSTEVQIQIIKNFLIGKQDDKIKKPGYLS"
     gene            3145..3903
                     /locus_tag="ACF2SP_002230"
     CDS             3145..3903
                     /locus_tag="ACF2SP_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2360005.1"
                     /translation="MTKLKNLDTYLSLCTEVYDLSKPNPPEEDYAFYRSYVINVKGPI
                     LEPMCGTGRFLLPLLEEGFEIHGFDASEYMLEALNIKAKAKNLKPTVWRGFVENLTRP
                     EKYNLIFIPSGSFCLLIDPATVRLALQTFYNHLSDEGILLFEGETLKAVPQLDVWRGS
                     VWHKPNGQMIMLSQLATLKDQVCHSIGKYELVHDNSIIHTEVEELRVRIYDPQELTAI
                     LKSCGFKNVRLIKAFDSAATPDAQDETIVYECRK"
     gene            3887..4354
                     /locus_tag="ACF2SP_002231"
     CDS             3887..4354
                     /locus_tag="ACF2SP_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360006.1"
                     /translation="MNAENNVLNTLNSLSKEINKLYGYVTIAGDNFGEPAINSGPCGP
                     FANAFYKIWNQKFTEKVNIAFIMMKNSDECWHILIRLPNGLLFDGGFGVHNEDKWNKD
                     TFEIEDMLEYDLQLLEKHSGGLNRTYPRYCPDFSIDVITNLIHKHLELVEKNK"
     gene            4692..5540
                     /locus_tag="ACF2SP_002232"
     CDS             4692..5540
                     /locus_tag="ACF2SP_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0518 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360007.1"
                     /translation="MTDLRTEIESLIHNELLPDREYVSAKRFINHYANTNEFWAGLAS
                     VGITPIINGLYACYYASRAVWATLRTVGNLLILKPGLALDAIKDVGCHLTLSTCLLLM
                     APIHALTHAVEILTRVISSWFVGQEARHDLLETGLMAKWANRVSGYQMVNELPSSNYF
                     NKARFFSSYNNAWDCLEQMASPMTSLAKGGFHSLVEAFYGVESAIECIVNLAICKPLH
                     AKENFHSLQVHFGLAVSLAVMTPINALVEGIAFMTRLGTTWVSACIKPEVTETEHENT
                     TAYQFN"
     gene            5979..6809
                     /locus_tag="ACF2SP_002233"
     CDS             5979..6809
                     /locus_tag="ACF2SP_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HFD2360008.1"
                     /translation="MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYY
                     EEGVGYALQQYYKKSDILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSL
                     EHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIEL
                     ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHRIIYQGFSLLTANQYELRHPKLENLS
                     KKYEKSIEQIIFRFSQQIGMLPLTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
                     "
     gene            complement(6799..7611)
                     /locus_tag="ACF2SP_002234"
     CDS             complement(6799..7611)
                     /locus_tag="ACF2SP_002234"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2360009.1"
                     /translation="MSMYDKRQFFKTYFSSASEKSILVKLLDKRFHPPSHTANILDLG
                     CHDGALMKNIVNAYAKRLPEKLVITGVDPSLQAIKEYAKADFPIPVQTNTFAGTAEDY
                     FDVCTDHFDWIFASQCLYWSPDLSKIIKQIHEAGDSALIVIRGCHGIYEIQSQFRHYI
                     GNHLEKFYTDKEVEMALRTQKILFQKESLSTSIRLPDKDSIELKWLILFFLQMEEESL
                     GDKIFCEVRDWITSRTSGSIHHEVHFFWLGDAIFTGDYNMNFPVKSKKNSRG"
     gene            complement(7647..8579)
                     /gene="legD2"
                     /locus_tag="ACF2SP_002235"
     CDS             complement(7647..8579)
                     /gene="legD2"
                     /locus_tag="ACF2SP_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegD2"
                     /protein_id="HFD2360010.1"
                     /translation="MDNLLNEKKLTIFTSNEIQDNLNRVLKNYEQNGIIGLNGYLNQA
                     NRISLLNELEIIQKDAEQDIVRYWNNKIIHFYSKDPLKPESVDREYVTEPYFQASSNK
                     AHVFYEVIDNMRVVNRIGHGMHLINRYPIMQRAVYDSPVLLDLLKGIGFKKPICHLSV
                     YIPKYPNGIGSEVKPHQESTFAFTEPQSVVVLWVALEDALIENACMYGVLGSNHWPLK
                     WVSKVDRETKTRHFEQVHQLHIPDFMTEREFYTALEVKAGDALLFHGNFVHCSPMNTS
                     KNSRKALSFQFIETYEVDYPETNWLYPPNKVYLY"
     gene            complement(8919..9182)
                     /locus_tag="ACF2SP_002236"
     CDS             complement(8919..9182)
                     /locus_tag="ACF2SP_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360011.1"
                     /translation="MADQPPKSKWHEIKRLSEGDNKVDNNQAPVSTADRIKKLYLDTQ
                     VGSPIDTVEEADAQRNNLGPKDTKIENSSPENDEPNPDIPTPG"
     gene            complement(9353..>9466)
                     /locus_tag="ACF2SP_002237"
     CDS             complement(9353..>9466)
                     /locus_tag="ACF2SP_002237"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFD2360012.1"
                     /translation="KYQQLKIKLAQEHKYDREQYTDAKSEFINQILKLAQN"
BASE COUNT         3007 a         1766 c         1744 g         2949 t
ORIGIN      
        1 agtcattaaa ttaattaaat aaacttcaaa taaatgcgga gtgtagctca gactggtaga
       61 gcactgcctt cgggaggcag gggtcgcagg ttcaattcct gtcactccga ccatataaaa
      121 tcaaaaatta gaattttcca cataatgaac ataaattttg aaaaagccaa tgcaagttat
      181 atcgagctta tctttagctg gcttaaagaa ccttttgttc aggagttctg ggacaacaca
      241 caagctcata aagatgacat cttaaacttt atcaatggcc gcacagaacc ctcatcctat
      301 gcggacggta aatatgttta ttggatagca agcggtaatg ggcatccttt cgcaatgctg
      361 atgaccatcc aggaaacaag cgaagatgag atcgacgaaa ttaaattgat taacctttcc
      421 aagactggac attcttatgg tatcgattac atgattggtg ataaaaacta ttttggcata
      481 ggttatggag ccagaacctt gatagaattt attgattttt ttataaaaga agtcgatccg
      541 aaagccgata cgtttttaat tgatcccgaa agtgacaatc ccagagccaa gcatgtttat
      601 ataaaagcag gttttgagta tgttgctgat tttttgatgt caggtgacgt ctctggcgcg
      661 ggaaaacttc atcatttatt aattaaacgc attggtttaa tcaaatgatt accggaataa
      721 atcacattac tttagcaatt aaagacctca ataagtcttt tgctttttat gctaacgtcc
      781 ttgggttcaa accattggtt agatgggata aaggcgctta ttttttagca ggcgattttt
      841 ggttttgttt aaatgtcgat gcaaatcaac gtcctacacc ttgctatact cattatgcct
      901 ttacagttac gcaagaacaa tttgatgaga tgtctaaggc gataattcaa tcaggcatta
      961 aaatctttaa agaaaatacg tccccagatg actcacttta ttttcttgac cctgatggcc
     1021 ataaattaga gatccatgcg ggaaactggc aatcgcgcct tattgcaaaa aaaatcaacc
     1081 caggtacatg gcaaaatgtg gagtggtttg tatgaaatca aaccatatca agcgtctatg
     1141 ccaacacttt aatttaggtg atccaacaca agaaccccaa agagtacctg gcggcttact
     1201 tcacatcatg tggcagctaa atacagataa aggttctttt gcggttaaac aattatcaaa
     1261 agacattaat cttacagatt atcatgttat tagaaattac gaattaagtg aaaacattgc
     1321 ctcaggcttt atagcacttg gtattcctgc tgttactgct attgaaaaat cgggcaaaca
     1381 tctttttata atcaatgaga ctggttttct ggtctaccca tgggtcaatg ctaaagccct
     1441 tgataaggat gttgtaagtg tatctcaagc tttggaaatt gctcggattt tatctaagat
     1501 gcaccagatc gatttgcagc tcaaggaaat tgaagaagca gaatttgata ttcatgaaaa
     1561 ggacaagatt atagctttaa tcgatttgtc ccaaaaaaag aagctcccta tcgcttcatc
     1621 cttgatggaa aagttagtaa caatattaga aattaataca aattatcatg atgcaatttt
     1681 aatgcttaaa gctcatagcg taattagtca cggcgacttg gatcaaaaaa atgtcttatg
     1741 ggattcctca aaaaaaccca tcctcataga ctgggagtcc gcacgcaagc ttaaccccac
     1801 ctatgaaatt gtgaatgccg cgttagattg gtctgggatt actacacaac ttgataaaaa
     1861 tctatttggc aaaatgctaa gagcatacaa agaagcaggc ggggtaattg ataaagacat
     1921 agttgaagct gctttttttg gtgttttagg taattggatt aattggatgg tctataacat
     1981 tcagcgggct gcaaaccatt gcgttccgga acaacaaaaa attggaatgg agcaagtaat
     2041 gcaagtgtta cctactgttc tacgcctcaa aagcttaata cctgaactcg tcaatgcagg
     2101 taaagcatga tcaccatcga tcttctcaaa aatcatccac aaagcatagc aaggtgtgcg
     2161 acaatttggc atgaagtact tgggaaaatc tgggttcccg agatcccggt tgagcaagtt
     2221 attcaacggt tcagtgagca cctaaatgct gacaaactac ccattacgtt tatagcactt
     2281 gatacaggga aacctgttgg gatgtgctct ttgcgtgaaa atgatggtat caggccagaa
     2341 ataagcccat ggcttggctc gcttgtggtt gaccctgtct atcagagaca aggtattgga
     2401 aacaagctaa ttgaagccac aaaaaataaa gccagggatt taggttttaa taaactgcat
     2461 ctttttgcct ttgacccaac cattcctaat tattactccc gtcttggttg gaccataatc
     2521 ggcatggata aatttaaagg acatccagtc actgtaatgg agatagtgtt atgactcctc
     2581 atattattgg cattagcggt ataacaggcg ctggaaaatc cactttggca aaagcattgt
     2641 ccctggattt aaaagcaact cttatttcat gggatgactt tgatgacatt tctttagaac
     2701 ccgaagatta tatcgagtgg tatcataagg gttgtaatta cagtgaattt caaagggaag
     2761 accttgcaaa agttttggca gagcttaaag caaaaagaga aattatgcat ccagtcttag
     2821 gcactttact caaccctgcg gaatacataa tttttgatgc tcccttaggg aaacttcacg
     2881 tacaaacagg tgaatatatt gatacctgtg ttcatattga ggtgccactt gatatttcat
     2941 tgtgccgtcg cattttgagg gattttaaag ataaattaca aactaaagaa aatcttcttg
     3001 aagaaatcag tttttattta aaccattcaa gacctttgtt ttttgatgat gaactgaaac
     3061 aaagtgccac tatagtcatt gacggtctcc taagtaccga agtgcaaatt caaataatca
     3121 aaaatttttt aattggtaaa caagatgaca aaattaaaaa acctggatac ttatcttagc
     3181 ttgtgcactg aggtctatga cttaagcaaa cccaatcctc ctgaagagga ttatgcattt
     3241 tatcgcagtt atgtaataaa tgtgaaaggc cctattttag agccaatgtg tggtaccggt
     3301 cgttttttac ttccattact tgaagaagga ttcgaaatac atggctttga tgcaagcgaa
     3361 tatatgcttg aagccctcaa tattaaagcc aaagctaaaa atctcaaacc aacagtatgg
     3421 agaggttttg ttgaaaattt aacgaggcca gaaaaataca atttgatttt tataccaagc
     3481 ggttcctttt gcttgcttat cgatcctgca acagtaagac tagcacttca aacattttat
     3541 aatcacctaa gcgatgaggg tattttattg tttgagggcg aaacactcaa agcagtgcca
     3601 caactcgatg tctggagagg ctcagtttgg cataagccca atggccagat gattatgctc
     3661 agtcaattag cgaccctgaa ggatcaggtt tgtcattcta tcggtaaata tgaactcgtt
     3721 cacgacaata gtataattca tacggaggtt gaagaattga gggtaagaat ttacgacccg
     3781 caggagttaa cagcaattct taaaagttgt ggttttaaaa atgtccgttt gattaaagca
     3841 tttgatagtg ctgcaactcc agatgcccaa gatgagacaa ttgtttatga atgccgaaaa
     3901 taatgttctt aataccctaa atagcttatc taaagaaata aataaactct atgggtacgt
     3961 caccattgct ggggacaatt ttggcgaacc tgcaattaac tcaggccctt gcggcccatt
     4021 tgccaatgct ttttacaaga tttggaatca aaaatttact gaaaaagtaa atattgcatt
     4081 tattatgatg aaaaattctg atgaatgctg gcatatctta atccgattac ctaatgggtt
     4141 actttttgat ggaggtttcg gtgttcataa tgaagacaaa tggaataaag acactttcga
     4201 aatagaagat atgttggaat atgaccttca gcttttagag aagcattctg gtggtttaaa
     4261 caggacatac ccccggtatt gccctgattt ttcgattgat gtcattacca atttaataca
     4321 caaacatctg gaactggttg aaaaaaataa gtgacaattt gctacccaaa gtctatttat
     4381 tgtttttaat tcgataaaac ttaccttgta cttcatgccc cactccatct ttgtagtatt
     4441 tcataccgca tttttccatc acaatatgtg ctgcattatg gccgattggg gcgtacgtca
     4501 taataaagaa aatttattca atgagttata cccaataaga gtattattga ttctatggcc
     4561 tttggtctat gttcaaaaga ccttggaaaa cagatgcaaa catcaaatac aacatgggcc
     4621 aatattggtc atttatgttt tttttggtta tactctcccc gcttgtttaa ttaacataga
     4681 gagagaccat aatgacagac cttagaacag aaattgaatc gcttattcac aatgaattat
     4741 tgccagatag agaatatgtt tcagccaagc gatttattaa tcattatgcc aatacaaatg
     4801 aattttgggc tggtttggca tcagtaggaa ttacccctat tatcaatggg ttatatgctt
     4861 gctactatgc ctcaagggca gtatgggcca ccttgcgaac tgtaggtaat ttattaattt
     4921 taaaacctgg cttggcattg gacgcgatca aagatgtggg ttgccattta actctctcta
     4981 cctgtttact gcttatggct ccaattcacg ctctgactca tgccgttgaa attcttacca
     5041 gagttatatc atcatggttt gttggtcaag aagctcgcca tgatttatta gaaaccgggc
     5101 ttatggcaaa gtgggcaaac agagtaagtg gatatcaaat ggttaatgag ttgccttcat
     5161 ccaattactt caataaagcc aggttctttt cttcttataa caatgcctgg gattgtttag
     5221 aacaaatggc atccccaatg acctccttgg ctaaaggcgg atttcattca ttagtcgaag
     5281 cattttatgg agttgagtct gcgatagaat gtattgtcaa tttggctatc tgtaagcctt
     5341 tacatgcaaa agagaatttc catagcttac aagtacattt cggtttggct gtctctttgg
     5401 ctgtgatgac accgattaac gctctggtcg aaggaattgc ctttatgaca cgtcttggta
     5461 ctacctgggt ttctgcttgc atcaaaccag aagtgactga gactgagcat gagaatacaa
     5521 ccgcttatca attcaactaa ttcttagctg cctgctacag tcgcttgcga ctgtagctca
     5581 ttaaaaaata ccaaggtctt tgtgattaag gactgatttc cttgtataat tctataaaat
     5641 tagtacgttc ttcgaataac ctttcgaatt aatcccgcct gttgtctttt aggcaaggat
     5701 ttgtaactat tgaaaatgaa aacaacagga cttacgaaaa accccaaggt caaggcaaaa
     5761 aatcttttta atgagggagt ttagataaac taaatgaccg aattaaaaac attttttaac
     5821 atagagattg gggtttttcg taagtcctga acaaatagta agacaagaaa tctcaggaaa
     5881 aaccctatgt aagacacaca gtcagctatg gtttgctctg ttttttgagt caaaatagag
     5941 tctgtttgca gataataaat cgagaaggag attgaacgat ggaccttggc tttccaactt
     6001 ttatatatgg aaccgcctgg aaagaaaagc gtacaagcga actggtcgag cttgcattaa
     6061 ctaccggatt tcggggtata gatactgcaa accaaagaaa acactattat gaagaaggtg
     6121 ttggatatgc cttgcaacaa tactataaaa aatcagatat cttgcgtgat gatatttttt
     6181 tgcaatccaa gttcacctat gctcatggcc aggatcatcg caaaccttac gatgatacag
     6241 caagcctgac tattcaagtc cagcaatctt ttgcaagcag ccttgaacat ttaaatactg
     6301 attatttaaa ttcctatatt ttgcatggcc cttattatca atatcatttt tctgatgaag
     6361 ataaagaagt ctggaataaa atggaatccc tgcataaaga tgggaaagta aaacacctgg
     6421 gcatatccaa tgtgagttat gaacagctaa tcgaactcat ttcatttgct tcggtaaaac
     6481 ccaagtttgt tcaaaataga tgctttgcca ggctattatg ggataaacaa attcgtaact
     6541 attgtcaggc ccatagaatt atttatcagg ggttttctct gttaacggcc aatcaatatg
     6601 agttaagaca tccgaaactt gagaatctca gcaaaaagta tgagaaatcg attgagcaaa
     6661 tcatttttag attttcacag cagataggaa tgttgccttt aactggcacg acaaatttgg
     6721 atcacatgaa agaagatctt gccatccatc agttcaggct gactgatgat gaaatcaatt
     6781 tcattgaaaa cattgccttt atcccctaga attttttttc gatttgactg ggaaattcat
     6841 attgtaatcc cctgtaaaaa tggcatcccc taaccaaaaa aaatgaactt catgatgaat
     6901 gcttcctgac gttcgagagg taatccaatc ccgcacttcg caaaatattt tatcacccaa
     6961 cgattcctct tccatttgca aaaaaaacaa aatcagccat ttcaattcaa tgctgtcttt
     7021 atcaggcaac cggatagaag tacttagaga ttctttttga aataaaattt tttgggtcct
     7081 taaggccatc tcaacttctt tatcggtata aaatttctct aaatgattcc caatataatg
     7141 cctgaattgc gattgaattt cataaattcc atgacatcct ctgataacaa ttaaagcgga
     7201 gtcccctgct tcatgaatct gcttaattat ttttgacaaa tcaggggacc aatacaaaca
     7261 ctgcgatgca aaaatccaat caaaatggtc agtgcacaca tcaaaataat cttctgcagt
     7321 tccagcaaaa gtattcgttt ggactggaat tggaaaatct gcttttgcat attctttaat
     7381 tgcttgcaaa ctaggatcca ctccggtaat aactaacttt tccggtaaac gctttgcgta
     7441 agcattcact atatttttca tcaatgcgcc atcatggcaa cccaaatcca atatgttcgc
     7501 agtatgagag ggaggatgaa atcgtttatc caacagcttt accaggattg acttctcgct
     7561 tgcgctagaa aaataggttt taaaaaactg gcgcttatca tacatagaca tgtagtgatt
     7621 cctcaatcgc ttatcaaatt taatttttaa tataaataca ctttgttagg agggtataac
     7681 caattagttt ccggataatc gacttcatac gtttcaataa actgaaacga caatgctttt
     7741 cttgagtttt tagacgtatt cattgggctg cagtgcacaa aattaccgtg aaataacaaa
     7801 gcatctccgg ctttcacttc caaggcagta taaaattcgc gctctgtcat aaaatcaggg
     7861 atatgtaact gatgaacttg ctcaaagtga cgcgtttttg tctctcggtc cacttttgat
     7921 acccatttca atggccaatg attacttcct aaaaccccat acatacaggc attctcaatt
     7981 aacgcgtctt ccagggcgac ccacagaaca accacacttt gtggttcagt aaaggcaaat
     8041 gttgactcct gatgaggttt tacctcactg cctattccat taggatattt gggaatatag
     8101 acactgagat ggcagattgg cttcttaaat ccaatccctt ttagcaaatc cagaagtaca
     8161 ggactatcat aaactgctcg ctgcataatc ggatacctat tgatgagatg cataccatgg
     8221 ccaattcgat tgactacccg catattatca atcacttcat aaaatacatg tgccttatta
     8281 cttgaagctt gaaaataagg ttctgtgaca tattctctat ccactgactc aggtttcaaa
     8341 ggatctttgg aataaaaatg aatgatttta ttattccaat acctgacaat atcctgctca
     8401 gcatcctttt gtattatctc aagttcatta agcaaactta ttcggtttgc ctgattgaga
     8461 tatccattaa gtccaataat gccattttgt tcatagtttt tcagaacccg gtttaaatta
     8521 tcttggattt cattagatgt aaaaattgtt aatttttttt cattcaataa attgtccata
     8581 acaactccaa accaatgtca tggctaaatt tgattgtaaa atgctaggaa ataaattacc
     8641 gtctgcttgc agacttaata tatatgggga atctggttta tctgacacgg ttatgacctc
     8701 tttaataaaa aataaagaag ccatcaccaa aaacctaatc gcgtgttcaa tcaggatgac
     8761 taaaagaata ctgaacacta aggattttct tgtaggaaaa tttacaagta tttattatgg
     8821 gtgtcaacac aaaaccaaca catgcgcttg ccgaaccaga atggtatagt taaacagcaa
     8881 aaaccatttt atttaaacaa tgtatttaat ttctgccatc atccgggggt tggtatatca
     8941 gggtttggct catcattctc aggggaggaa ttttctattt tagtatcttt aggccctaaa
     9001 ttatttcgct gggcatccgc ttcttcaaca gtatcaattg gactgcctac ctgtgtgtcc
     9061 agataaagct ttttaattct atccgccgtt gacactggtg cttgattgtt atctacttta
     9121 ttatcacctt ctgatagacg tttaatttca tgccatttgg attttggagg ttgatctgcc
     9181 attgcgtttc tcaactaaag taatttagta caattataga tcacaatgtt tgtagatcac
     9241 aacattaaga ttaggacttc taaaaacgtt gatgcatcgt ttagatctaa ccctgggcca
     9301 aaaaatccaa gttcgttaca cccatttatc cttgtctgcc catgataatt tattaatttt
     9361 gagctaattt taaaatttgg ttgataaatt cactttttgc atcagtgtat tgctccctat
     9421 catatttgtg ttcctgagcc agtttgattt ttagttgctg atattt
//
LOCUS       DAXLDT010000114         9335 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXLDT010000114 DAXLDT010000000
VERSION     DAXLDT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.114"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..707)
                     /locus_tag="ACF2SP_002238"
     CDS             complement(<1..707)
                     /locus_tag="ACF2SP_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD2360013.1"
                     /translation="MIWRVLTIVAVALISNQLKAFSWSDLWFTPNQQAQHLMEKGQFA
                     KAKDLFERNDWAASAAYRAGDYEQAAKLFNELKTEQGYYNQGNALAHLGKYEEAIRAY
                     DKALAFNPNNQDALYNRKLISELLKKDKEKKKSQNQQDKDQQNKDQQNQDQQNQDKQN
                     KNQQNQDQQNQDKQNKNQQNQDQQNQDKQNKNQQNQDQQNQDKQNKDQQNQDQQNQDK
                     QNKDQQNRDQQNKAQQN"
     gene            complement(704..1636)
                     /locus_tag="ACF2SP_002239"
     CDS             complement(704..1636)
                     /locus_tag="ACF2SP_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFD2360014.1"
                     /translation="MIAEFHFLRPWCLLMIVPLLALVVIYWRQKPKLHAWEAVCDPHL
                     LEHLLHSKGQSKRALSLSFLFFSILFMILSVSGPTWYKLPQSTYKPIQPRVLLLDMSD
                     KMMLGDLSPNRLSRAKFKLHDLFTRKDIGQFGLIVFTGEPFVVSPLTDDGQTISSLLS
                     TLTPDIMPIAGYNLDSALDEASQLIKQAGYDHGEILVLTAETPSSSAIGKAKKLAESG
                     IFSSIMPIRADENLNPLFQRFANAGDGYLLVYSPDSSDLEKWLNASQGKEKFALEKND
                     DIPLWRDEGRWFLIPALLLLLPVFQRGWMQRVTI"
     gene            complement(1626..2660)
                     /locus_tag="ACF2SP_002240"
     CDS             complement(1626..2660)
                     /locus_tag="ACF2SP_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFD2360015.1"
                     /translation="MFELATPWALLLIPVPLFLWYLLPRAKVKLPAALKVPFFEAMAG
                     IVEQENRLISAKTLLLIPVLVWVLLVIALSGPRWVGEPKPVAREGYNIMMVLDLSGSM
                     EITDMLLHGRPVSRLLVVKRAAEQFVEDRVGDRIGLILFGTRAYLQTPLTYDRHSVLM
                     RIDDATAGLAGKTTSIGDAVGLAVKRLQDVPSKGRVIILLTDGANNSGVLAPLKAAEL
                     AKQDGIKIYTIGLGSEADPRALTGDFFAPTLSAELDEKTLEEMAKMTGGRYFRATDPE
                     SLQSIYQTINQLETVKQEQATVRPQKEYYPWFVAFALLLCFYWLISKADLFVYLRSFL
                     KVERSLSHDS"
     gene            complement(2648..3133)
                     /locus_tag="ACF2SP_002241"
     CDS             complement(2648..3133)
                     /locus_tag="ACF2SP_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HFD2360016.1"
                     /translation="MAKTETLAQLRDIHLPEPVGWWPLAPGWYGLMVLIVVLVAGVAY
                     FIYKRHVNALPKKQALSLLKIHKEQYEKDKNTQLASAHISELLKRVALVYYPRAEVAS
                     MHGEAWVEFLNQTGKGIDFTPVKSMLLDSPYKTSDALNLNPLFTRAEKWIKQRGAPCS
                     N"
     gene            complement(3126..4037)
                     /locus_tag="ACF2SP_002242"
     CDS             complement(3126..4037)
                     /locus_tag="ACF2SP_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFD2360017.1"
                     /translation="MTDGVVAELSELIDLRRYVHSVHYKPQGRALRSGNHISKFRGRG
                     MDFAEVRNYQAGDEIRHMEWRVTARTGRPHVKIYQEERERPVVLLVDFNPSMIFGTKI
                     AFKSVVAARLASLLAWTVIKQGDRVGGFFFSATEHSEFIPRGRDSGVLPLLASLSRYT
                     TQTEAQQEEKPRMLSDALMRLRRVIRPGSILVLISDFYSMDVECEKHLNRLRGHNDIL
                     AYHVCDRIELAPPKPQQYAISDGKQEIILDTGLETVSTAYQLYCQQRIEQLNDQFKRL
                     QIQYVQVTADTNLAQLVRFTFPRRSRG"
     gene            complement(4047..5042)
                     /locus_tag="ACF2SP_002243"
     CDS             complement(4047..5042)
                     /locus_tag="ACF2SP_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD2360018.1"
                     /translation="MEQLDDKVADNVQQKILQLSRHLNSRILGQKDLISRLLIALLAD
                     GHLLVEGAPGLAKTRAVKELSDVVEGDFHRIQFTPDLLPGDLTGTDVYHPQNGSFVFQ
                     PGPIFHHLILADEINRAPAKVQSALLEAMAERQVTIGGKTYPLPELFLVMATQNPIEQ
                     EGTYPLPEAQLDRFLMYVKISYPDAKVEHDILTLARKEALGNATENQIKVTKLSQKTL
                     FEARKQVLKVHTSEALENYLVQLVVATRNPGVYSDELGRWLRFGASPRATIALDRCSK
                     AHAWLAGRDYVTPDDIHVIAHDVLRHRILLSFEAEAEGISSDDFIDSLLRLVAIP"
     gene            complement(5219..5662)
                     /locus_tag="ACF2SP_002244"
     CDS             complement(5219..5662)
                     /locus_tag="ACF2SP_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360019.1"
                     /translation="MDRRYLSPLELLGIATQHAYTADYMLQQIANGMYRGGETIAVFS
                     PITSLMYVAFQLTFKAYCLHDHRPIKEYKNLMELVELNSHLGLSSNDIFLLKTLSRQQ
                     VFNKGVDYDLWENQQQLHVFCEEIISLYERVQSMMPLELQSDYQE"
     gene            5744..6730
                     /locus_tag="ACF2SP_002245"
     CDS             5744..6730
                     /locus_tag="ACF2SP_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HFD2360020.1"
                     /translation="MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPA
                     YCVSEMCSAIDILHKHSSQSRYIYRAPTEKLLAYQISGNEPHVLAEAAAKLQSLGADL
                     IDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
                     AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDISNISSLRKA
                     LEISMCDGFMISRAGTGKPWLYRDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKA
                     VLQSKSLVRYYFKKQLNEQQLQLFYQLGTLMEIKDFLAEATHRVSNILVATS"
     gene            complement(6789..7148)
                     /locus_tag="ACF2SP_002246"
     CDS             complement(6789..7148)
                     /locus_tag="ACF2SP_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360021.1"
                     /translation="MSKWILLSGSFFLCLFSLSVHSHSFDKEQLVQRCQILHDELKEL
                     ESHQYNGVCRHKLALAANKIFSAKIRIVYENYKGAKQDLSVSMNNMKFAEDISCVFKS
                     GITKARMEAREIQRELN"
     gene            complement(7205..7960)
                     /locus_tag="ACF2SP_002247"
     CDS             complement(7205..7960)
                     /locus_tag="ACF2SP_002247"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HFD2360022.1"
                     /translation="MNQTNTQEAKVALVTGGAKRIGAAIVKKLHLSGYQVAIHCHSAL
                     HEADILAKHLNQVRMNSAIVLQKELTEPGAAEEIISKVKHWAGRLDLLVNNASVFKRT
                     DCHFLNEADWQYLFAVNVQAPFLLSMAACPLLAPCKGSIINITDIHADKPLKGYSVYC
                     QTKAALEMQTRSLARELAPEIRVNAIAPGAIAWPENDNSLTAEAQQKIIEKTPLKRHG
                     SPDFIAQAVLSLAENTFITGQTLKVDGGRSLTS"
     gene            8137..9249
                     /locus_tag="ACF2SP_002248"
     CDS             8137..9249
                     /locus_tag="ACF2SP_002248"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFD2360023.1"
                     /translation="MNLKQTLINQLKLQKEIPFIDFMQQALYAPYEGYYTSGLQKLGK
                     HGDFITAPELTSLYGKTLANQFQQILPLLDSPVLFEFGAGTGKLCIDTLTHLEQLQCL
                     PESYFILEVSANLRHRQQELIQQKIPQLAGLIHWLDKWPETPFNGVIIANEVLDAMPV
                     YRFMQSETEILESYVALDEHDQLAEIFKPVQNQRLLAYIKNRLPPLDYPYLTEANLFL
                     DDWLLNCSHMLKKGALFIIDYGFPRHEYYHPDRNQGTLMCHYQHYSHPNPLLNPGEQD
                     ITSHVDFTHVAEAGHQAGFHVAGYTNQASFLLANGLLSLIHTLDDEQELFVAKQAIKQ
                     LTQPSEMGELFKVIALTKDLEIDLNGFLINDKRVNL"
     gene            9252..>9335
                     /locus_tag="ACF2SP_002249"
     CDS             9252..>9335
                     /locus_tag="ACF2SP_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydrobiopterin synthase"
                     /protein_id="HFD2360024.1"
                     /translation="MKKYLTTVELQKESMKFSAGHTTIFSAT"
BASE COUNT         2756 a         2058 c         1725 g         2796 t
ORIGIN      
        1 ttattctgtt gtgccttatt ttgctgatcc cggttctgct gatctttgtt ttgcttgtcc
       61 tgattttgtt gatcctgatt ctgttgatct ttgttttgct tgtcctgatt ttgttgatcc
      121 tggttctgct gatttttgtt ctgcttgtcc tgattttgtt gatcctggtt ctgctgattt
      181 ttgttctgct tgtcctgatt ttgctgatcc tggttctgtt gatttttgtt ctgcttgtcc
      241 tgattttgct gatcctggtt ctgttgatct ttgttttgtt gatccttatc ctgctgattc
      301 tggctttttt tcttttcttt atctttcttc aacaattcac tgattaattt tcgattatat
      361 aaagcatcct gattatttgg attaaatgcc aaggctttgt catatgctcg tattgcttcc
      421 tcatatttcc ccaagtgtgc taaagcattt ccctgattat agtagccttg ttccgttttt
      481 aactcgttaa ataatttggc tgcctgttca taatctcctg ctctgtatgc tgcgcttgca
      541 gcccagtcat tgcgctcaaa aagatcttta gcttttgcaa attgcccctt ttccatcaaa
      601 tgttgagctt gttgattggg agtaaaccat aaatcagacc aactaaatgc cttaagctga
      661 ttggatatta gtgcaactgc cacaatagtt aaaacccgcc atatcatatc gtcacccttt
      721 gcatccaacc tcgctgaaat acgggtaata ataaaagcaa tgcgggaatc aagaaccaac
      781 ggccttcatc acgccataat gggatatcgt catttttttc aagagcaaat ttttctttgc
      841 cttgcgaggc gttcaaccac ttttccaaat cacttgaatc tggggagtat actaacaagt
      901 atccatctcc cgcattggcg aagcgttgaa acaaagggtt caaattttca tcagctctga
      961 tcggcataat tgaagaaaaa atacctgatt ctgccagctt tttagctttt ccaatggcgc
     1021 tagaggatgg tgtttcagcg gttaaaacta aaatttcgcc atggtcataa cccgcttgct
     1081 taattaattg gcttgcttca tccaaggcac tgtctaaatt atatcctgca atgggcatga
     1141 tatcaggggt caacgttgac aataaggaag agatagtttg accatcgtct gttaaaggag
     1201 atacaacaaa aggctcgcct gtgaaaacaa tcaaaccaaa ctgccctata tccttgcggg
     1261 taaataaatc atgcaatttg aatttggccc gactaagacg atttggcgat aaatccccaa
     1321 gcatcatttt atctgacata tccaataaca aaactcttgg ctgaattggc ttataagtag
     1381 attgaggcaa tttgtaccaa gttggaccag acacactcaa aatcataaat aaaatactaa
     1441 aaaacaggaa tgataaagat aaggcacgtt tactctgacc tttactgtgt aataaatgtt
     1501 ccagaagatg gggatcacat accgcctccc aggcatgtaa ttttggtttt tgccgccaat
     1561 aaattaccac caacgcaagc aaaggcacta tcatcaacaa acaccatggc cttaaaaaat
     1621 gaaactcagc tatcatgact taagctccgc tctactttta aaaaagacct taagtacaca
     1681 aataaatccg ccttgctaat caaccaataa aaacataata acaaagcaaa ggcaacgaac
     1741 cagggataat attccttttg aggccgaaca gttgcctgtt cttgcttcac ggtttccagt
     1801 tgattgatgg tttggtatat ggattgtaaa gactcaggat ctgtcgccct gaaataacga
     1861 ccacctgtca tttttgccat ttcttccaat gtcttttcat ccagttcggc ggaaagggta
     1921 ggcgcaaaaa aatcaccagt taacgctctt ggatcggcct ccgaacctaa gcctatagta
     1981 tagattttga ttccatcttg cttggctaac tctgctgctt ttaagggggc taatacacct
     2041 gagttgtttg ctccgtccgt cagcaaaata ataactcttc ctttggaagg aacatcttgt
     2101 aaccttttaa cggctaaacc taccgcatca ccaatcgaag tagttttacc tgcaagacca
     2161 gctgttgcgt cgtctatcct cattaatacg ctatgtctgt cataggttaa tggtgtttgc
     2221 aaataagctc gagtaccaaa gagaatcaaa ccaattctat ctccgactct gtcctcaaca
     2281 aattgctctg cggccctttt cacgacaagc aaacgactga ctggtcggcc atgcagcagc
     2341 atatccgtaa tttccatgct gccagaaaga tccaatacca tcatgatatt atatccttcc
     2401 cttgctacag gctttggctc accaacccat cgcggaccag aaagggcgat aaccaacaat
     2461 acccaaacta aaacaggaat taatagcagc gttttggccg agattaaccg gttctcttgt
     2521 tcaacaatac ccgccattgc ctcaaaaaaa ggaaccttca atgcagcagg taattttact
     2581 tttgcccgcg gtaacaaata ccagagaaac aaagggacag gaatgagtaa caaagcccat
     2641 ggggtagcta attcgaacac ggcgcccccc tttgcttgat ccatttttct gcacgagtaa
     2701 ataaaggatt taaattcagt gcatctgatg ttttataggg tgaatccaat aacatcgatt
     2761 taaccggagt aaaatcaatc cccttgccag tctgatttaa aaattcaacc caagcttcac
     2821 catgcatact ggcaacttct gctcttggat aataaaccaa ggcaactcgc tttagcaatt
     2881 cagaaatatg ggcactggcc aattgagtgt ttttatcttt ttcatactgt tctttgtgaa
     2941 tttttaataa acttaaggct tgtttcttgg gtaatgcgtt aacatgcctt ttataaatga
     3001 aataggcaac ccctgccact aaaacaacga taagaaccat taagccatac caccctggcg
     3061 caagaggcca ccagccaaca ggctcaggca aatgaatgtc tcgtaattga gctaatgttt
     3121 ctgttttagc cacgagacct ccggggaaag gtaaagcgca ccagttgagc gagattggta
     3181 tcagcagtca cctggacata ctgaatttgc aaccgcttaa attgatcatt taactgctca
     3241 attctttgct gacaataaag ctgataagct gtgctaacag tttccagacc agtgtctaat
     3301 atgatttcct gtttaccatc gctaatggca tattgttgcg gctttggagg agccagttca
     3361 attctgtcac aaacatgata ggccagtatg tcattatggc ctcgtaaacg atttaaatgc
     3421 ttctcacact caacatccat agaataaaaa tcgcttatca aaaccagtat acttccaggc
     3481 cgaataaccc gacgcagcct cattaaggca tcacttaaca tacgtggctt ttcttcttgc
     3541 tgagcctctg tttgagtggt atatcgactt aaagaagcca ataaagggag tacgcccgaa
     3601 tctcgtcctc gcggaataaa ctcactgtgt tctgtagctg aaaaaaagaa ccctcctaca
     3661 cgatctcctt gttttatcac tgtccatgct aaaagagaag ccaatcgtgc agctaccact
     3721 gacttaaatg ctatcttggt gccgaaaatc atggaagggt taaaatctac aagcagaacc
     3781 actggccttt cccgctcctc ctgataaatt ttgacatgag gccttccagt acgggcggta
     3841 acgcgccatt ccatatgacg aatttcatca ccagcctgat aattccttac ttccgcaaaa
     3901 tccattcccc gtccacgaaa tttggaaata tggtttccag aacgtaatgc tcttccttgc
     3961 ggcttataat gaacggaatg aacataccgt cttaaatcga ttaattcgct taattcagct
     4021 acaactccgt cagtcataaa aaacctctaa gggatggcga ctaaacgtaa taaagaatct
     4081 ataaagtcat cgctgctaat gccttctgct tctgcttcaa aacttaacaa aatcctgtgg
     4141 cgcagtacat catgagcgat aacgtgtata tcatcgggag tcacataatc tctacctgca
     4201 agccaggcat gagccttgga acaacggtcc agtgcgatag tagcgcgtgg gcttgctcca
     4261 aaacgcaacc agcgtcccaa ttcgtcactg tatacaccag gattacgggt agcaactacc
     4321 aactgaacca gataattttc aagtgcttca ctggtatgca ccttcaatac ttgttttcga
     4381 gcttcaaata aagttttttg ggacaatttt gtcactttga tttgattttc tgttgcatta
     4441 cccagtgctt ctttacgtgc caaagttaaa atgtcatgtt ctacttttgc atcagggtaa
     4501 ctaattttca catacattaa aaatctgtcc aattgggctt ctggtaaagg ataagttcct
     4561 tcttgttcta taggattttg agttgccatg actagaaaca attccggcag aggataagtt
     4621 tttcctccaa tagtgacttg cctttctgcc atcgcttcaa gcagggctga ctgtactttg
     4681 gcaggcgcgc ggttgatctc atcggccagg ataagatgat gaaatatagg gcctggctga
     4741 aagacaaaag aaccattttg cggatggtag acgtccgtac cagttaaatc gccaggtagc
     4801 agatcggggg tgaattgaat ccgatgaaaa tcaccttcaa ctacgtctga caattcttta
     4861 acggctctgg tttttgctaa acccggagct ccttcaacca acaagtggcc atccgcaagc
     4921 aaagcgatca acagacgaga gatcaaatcc ttttgaccca aaatacgcga attaagatga
     4981 cgacttaact gtagtatttt ttgttgcaca ttatctgcga ctttatcgtc tagttgctcc
     5041 atgacaatta cccttttaaa aaaataatat cctaacttga atccattggg atgccaagta
     5101 gaatgagtct attttggata aaaataaatc attttgatat ctgattaatg catcctgggc
     5161 catgcacaac ataagaaaac ttaagcaaag acacttaaca gggcgctaat tctgccggtc
     5221 actcctgata gtcactttgc aactccagag gcatcatcga ctgcactctc tcatacaatg
     5281 aaataatttc ctcacaaaaa acatgcaatt gttgctgatt ttcccaaagg tcgtaatcta
     5341 ctcctttatt aaacacttgc tgtcttgata aagtttttaa aagaaaaata tcattgctgg
     5401 ataaaccaag atggctattc aactcaacca gttccatcag atttttatat tcctttattg
     5461 gtctgtgatc atgcaaacaa taagctttga aggttagctg aaaagcaacg tacatgagtg
     5521 aagtgattgg agaaaaaact gcgatggttt cgccacctcg atacattcca ttggcaattt
     5581 gctgcaacat ataatctgct gtataggcgt gttgcgtagc aatgcctagc agctccaaag
     5641 gtgacaaata cctcctgtcc atatggaagc ctctcccttt tttctggttc ttcatgatga
     5701 atcatgttaa aataaaaagc gattatatcc tttaattcca ttgatgcaca cttttattaa
     5761 cagtcctctg caaatcggct ctttaaaact ccctcatcga ctgattcaag gacctttggc
     5821 aggatacagt tgcgcccctt tcagagatct attcaatatt tatacaccgc ctgcttattg
     5881 cgtttctgaa atgtgttctg ccatcgacat tcttcataaa cactcctctc aatcccggta
     5941 tatttacaga gctcccacag aaaaactcct ggcttaccaa atctccggaa atgagccaca
     6001 tgtccttgca gaagcagcag ctaaactgca atcactgggt gcggatctaa ttgatattaa
     6061 ttgcggctgc cctaaaagca aaattcgtaa aaaaggggct ggtagcgctt tattggaaga
     6121 tccacaaaaa ttgattgcca ttatctctag ggtacgcgca gcaattagca ttccgctgac
     6181 tattaaaata cgcattcaaa ataatgaaaa ggatttaacc ttagccaaac aaatagagga
     6241 cgccggagct catgccctga tagtacatgg caggcgatgg acagaagatt atgatatccc
     6301 atgcaattgg cagcaaattg caaatatcaa gaatgctgtc aatataccag ttattgtaaa
     6361 tggtgatatt agcaatatat ccagtttaag aaaggccctg gaaatcagca tgtgcgatgg
     6421 ttttatgatt tctcgagcag gtacaggcaa accctggcta tatcgagatt tattgaatct
     6481 ggaaacaaat gatgtcgctt ttgacgagaa attaaaactg tttatgctcc acctcgaagg
     6541 attatcatac cttgaaaacg agcataaagc ggtactgcaa agcaagtctc tagtccgcta
     6601 ttatttcaaa aagcaactga acgaacaaca actgcaactg ttctatcaat taggtacatt
     6661 aatggaaatc aaggattttt tagctgaggc aactcaccga gtttccaaca tattagttgc
     6721 cacctcatga tataaggtca ggatttgtat attatttcta taatttgata accattacat
     6781 tccgctattt agttcagctc acgctgaatt tctcgggctt ccattctggc cttggtaatc
     6841 cctgacttaa aaacacaaga aatatcttct gcaaatttca tgttattcat tgatactgaa
     6901 agatcttgtt tagcgccttt gtaattctca taaacaattc taatttttgc agagaatatc
     6961 ttatttgcag ctaatgccag tttatgccga cataccccat tgtactgatg agattccaac
     7021 tcctttaatt catcatgaag aatctggcat ctttgcacta actgctcttt atcaaaagaa
     7081 tgggaatgaa cacttaaaga gaataggcat aaaaaaaagg agccagataa taaaatccat
     7141 tttgacataa taataatccc tatatagtaa attgagcatt tgaaatgaaa tccagtatac
     7201 aactctagct tgtcaaactt ctacctccat ctactttcaa tgtttgacca gtgatgaatg
     7261 tattttcagc taaagagaga acagcctgcg cgataaaatc aggactgcca tgccttttta
     7321 atggtgtttt ctcgataatt ttttgttgtg cttcagcagt taaactattg tcattctcag
     7381 gccaggcaat ggcgccaggc gcgatggcat tgactcttat ttctggcgcc aactcacgag
     7441 caagcgacct cgtttgcatc tccaatgcgg ctttagtttg acaatatacc gaatatcctt
     7501 taagtggctt atctgcatga atatcagtta tgttaataat ggaacccttg catggagcta
     7561 gtaaaggaca agctgccata ctcagtagaa agggggcctg gacattcact gcaaacagat
     7621 attgccagtc tgcctcattc aaaaaatggc aatccgttcg tttaaaaaca gaagcattat
     7681 tgaccagcaa atccaaacgg cctgcccaat gtttcacttt ggatatgatc tcctctgctg
     7741 cgccaggctc agttaattct ttttgcaaga caatggcgct attcattcgt acctgattca
     7801 aatgtttggc cagaatatct gcctcatgca aagcagaatg acaatggatg gctacctgat
     7861 atccagagag atgtaatttt ttaacaattg ctgcgccgat acgtttagcg cccccggtca
     7921 ctaaagcaac cttggcttct tgcgtgtttg tctgattcaa ggtaggttcc ccatatggta
     7981 acagactcct ttcgcaacct cactgcaaaa agtaatcagc caggatgctc gaatcctcaa
     8041 gtattattgt gtacacttca gtattttctt tgtttctcct cgcaccctct tgttcacagc
     8101 gagtttaaaa gaagtctaac gatagtttaa gtgattatga atttaaaaca aactctaata
     8161 aaccagttaa agctgcaaaa agaaatccct tttatagact ttatgcagca agcactctat
     8221 gccccctatg agggctatta tacttcagga ctgcaaaagt taggaaaaca cggtgatttt
     8281 atcactgctc ctgaattaac ctccttgtac ggcaaaactt tagccaacca attccaacaa
     8341 attctgccct tgctggattc ccccgtacta ttcgagtttg gggccggaac tggaaagctc
     8401 tgtattgata cattaactca tttggaacaa ttacagtgct tgcctgaatc atatttcatc
     8461 ctggaagtaa gtgctaactt acgtcatcgt cagcaagaat tgattcaaca aaaaatccct
     8521 caattggccg gcttgattca ctggctggac aaatggccag aaactccttt taatggagta
     8581 ataatagcaa atgaagtact ggatgccatg ccagtttatc gctttatgca atctgaaaca
     8641 gaaatcctgg aaagctatgt cgctctggat gaacatgatc aattagcaga aatcttcaaa
     8701 ccagtacaaa atcaacgact attggcctat atcaaaaatc gtttaccacc actggattat
     8761 ccttacctta ctgaggctaa tttgtttctt gatgattggt tgttgaattg cagtcatatg
     8821 ttgaaaaaag gggctttgtt tattattgat tatggcttcc ctcgccatga atactatcac
     8881 cctgatcgta atcaaggcac gttaatgtgt cattaccaac attattctca tcccaatcct
     8941 ttacttaacc cgggagaaca agatatcact tcacatgttg attttactca tgttgctgag
     9001 gcgggacacc aggcgggatt tcatgttgca gggtatacta atcaagcctc ttttttgctg
     9061 gcgaacggtt tgctaagctt aatccatact cttgatgatg aacaggaact gtttgtggca
     9121 aaacaggcca ttaaacagct aactcaaccg agtgaaatgg gagagctatt caaagtcatt
     9181 gcgttaacca aagatctgga aattgattta aatggttttc tgataaatga taagcgagtg
     9241 aatttataat tatgaaaaaa tatctgacta ctgtggaatt acagaaagaa tccatgaagt
     9301 tttctgccgg gcataccacc atcttttcag caaca
//
LOCUS       DAXLDT010000115         9309 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXLDT010000115 DAXLDT010000000
VERSION     DAXLDT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.115"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..254)
                     /locus_tag="ACF2SP_002250"
     CDS             complement(<1..254)
                     /locus_tag="ACF2SP_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HFD2360025.1"
                     /translation="MSVEIQQASSTNPNITFSESAIKHLVTYLEKNPGNKGVRLSVKK
                     TGCSGLSYVVDYVKEPSEGDMIASLSTDYLVCVDKTSYPFL"
     gene            complement(259..642)
                     /locus_tag="ACF2SP_002251"
     CDS             complement(259..642)
                     /locus_tag="ACF2SP_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HFD2360026.1"
                     /translation="MMYNKIVQDYFFQPQHIGKLDLNDPFVIGVQSQPINQLNTVHFY
                     IKCDGDKLISKARFKTNGNPYVIAAMEWICCQIEGKTLESVPLIDFQILINVLQITNN
                     QYPVAVQVADIYKEALTLMRKKFER"
     gene            complement(639..1796)
                     /locus_tag="ACF2SP_002252"
     CDS             complement(639..1796)
                     /locus_tag="ACF2SP_002252"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFD2360027.1"
                     /translation="MKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGR
                     AASMAVEQARSQIAETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVTMSTE
                     HKAVLDSFHQLEKEGFQVTYLHPESDGLLDLGKLESALRPDTILVSVMHVNNEIGVIQ
                     DIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVR
                     HKPRIRLQPLSYGGGHEGGLRSGTLPTHQIVGMGEAFAIAETDRIPEQTRILNLRKQL
                     WDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSELAISTTSACSSASIQP
                     SYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICRQVSRLHELSPL"
     gene            complement(1777..2550)
                     /gene="trmJ"
                     /locus_tag="ACF2SP_002253"
     CDS             complement(1777..2550)
                     /gene="trmJ"
                     /locus_tag="ACF2SP_002253"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFD2360028.1"
                     /translation="MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPD
                     LKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELIN
                     QQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
                     LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRIN
                     LEKMEVSILRGMLSQVQKSLEWARKDNRSNRIGNKTDLF"
     gene            2666..3451
                     /locus_tag="ACF2SP_002254"
     CDS             2666..3451
                     /locus_tag="ACF2SP_002254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFD2360029.1"
                     /translation="MEPLLNIAVNAARQAGEIINRYVEQVDRLKITPKNSHEYFSEVD
                     IKAEQVIINTIHKAYPEHGILAEESGIQQSDSDTVWIIDPLDGTSNYLHGFPFYSVSI
                     ALKIKNRLEHGVIYDPLRHECFAASRGRGARLNDRRIRVSKQTQLNASLLGTGFHFRD
                     ATLAQRYLPTFEALIGKCAGVRRTGSAALDLAYVASGRLDGFWEFGLRPWDIAAGALL
                     VREAGGLISDVQGGEDFLNYGDVVAGTPKVFKSLLQTISPVLK"
     gene            complement(3564..4520)
                     /locus_tag="ACF2SP_002255"
     CDS             complement(3564..4520)
                     /locus_tag="ACF2SP_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFD2360030.1"
                     /translation="MNNESSSNSNLDSQALLNQLVIDYLQERKRKRRWKWIIRTIILV
                     VLVYSSYKIVSVAKTTEASKDKEHIGLIDINGEIADSSSASADNFTKGIEKAYRSKGL
                     KALIVRINSPGGSPVQAEYIYNVLQYYKSLKPNIKTYAVCVDMCASAAYYVAVGADEI
                     YASPASMVGSIGVLYNGFGFVDALQKLGISRRLQTSGVNKAFLDPFSPTTEFQKEKLQ
                     TMLDIVHKQFITRVKEGRGNRLHVDDETFSGLFWTGEQALANGLIDGYASSGQLAREV
                     IKITDIVDYTYKQNVFDRLTKNLGTAMADELPVILGMKPGLR"
     gene            4609..7185
                     /gene="clpB"
                     /locus_tag="ACF2SP_002256"
     CDS             4609..7185
                     /gene="clpB"
                     /locus_tag="ACF2SP_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFD2360031.1"
                     /translation="MRMDKLTSKFQMALADAQSLALGRDNGFIEPEHLMKALLDQQGG
                     SCRPLLSKAGVNIPLLRTLIDQALDKLPKVSGTGGDIHISNALNRLLNLTDKLSQQRK
                     DNFISSELFVLAAINEDSNLAKILKQAGGDNKAIEKAIDELRGGETVNDPNAEEQRQA
                     LEKYTLDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKNKRLLALDMGALIAGAKYRGEFEERLKGVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPALVAAAMLSHRYISDRQLPDKAID
                     LIDEAASLIRMEIDSKPESMDKLERRLIQLKIEREALKKENDEASKKRLVDLQKSIDE
                     LEQNYSDLEEIWKAEKATMQGSTQIKEALEQAKLEMETARRAGDLSRMSELQYGRIPE
                     LEKRLSQVSSVDAMETKLVRNKVTEDEIAEVVSKWTGIPVSKMMEGEKEKLLKMEEAL
                     HSRLIGQNEAVDAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALASFLFD
                     TEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HTDVFNILLQVMDDGRLTDGQGRTVDFRNTVIVMTSNLGSQLIQEMSSKFNYDEIKAA
                     VMDLVSQHFRPEFINRIDESVVFHSLTKEQIAKIAAIQINYLHHRLKQQNITLEVTSE
                     ALSHLAEAGFDPVYGARPLKRTIQQKLENPLAQSLLTGKFKSGDTIIVSYKDGVMEFS
                     KQ"
     gene            complement(7277..8590)
                     /locus_tag="ACF2SP_002257"
     CDS             complement(7277..8590)
                     /locus_tag="ACF2SP_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1751 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360032.1"
                     /translation="MTFTPPEFKILSVNTRNLETVFSTLLGRYKIITDPPVSEAVSSG
                     EIISNTLETLLARTHKVVICKTDRETARDVFKQLSNELREVLKENNEEKNKQAILFLL
                     GALLHRYFRLIKEYDNFNSYIPVPSFFFKYKPPSDVKDCRLFQAIRLALGLPEVMEKN
                     YRINDLKILDVTTIVTALETFRDNMQLIIGKDEGKMPRYKSYPHFAADKNFETYLQEI
                     IDEHKRRNPVVLNQFKAINFIQSLVKQIEEEQHQIEEALTHLGKLLPKTCSDFKTISL
                     ELMEEQIKAQIESNVLQEKIIDLLYTGHIQENFSTMDCGSFIEAMKNCNNSLARYRAL
                     GGYCLLLQNEGVKEQLRFCIHQALGVEINPNELTDKDMLDAIRLLKTYFEANPKVELN
                     FDFFGGKGSMNTFILQTELALAKKVQTVNKAQEDNSETRTTSLFV"
     gene            complement(8603..9250)
                     /locus_tag="ACF2SP_002258"
     CDS             complement(8603..9250)
                     /locus_tag="ACF2SP_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1752 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360033.1"
                     /translation="MRFLRVGPCGRETMLEFAEFQQNALKLTDQLKKRSIPSLGCFCF
                     FSVILPNTAAIEDTHTIKLVAYSLDSENDDDLIAYLKGVTRYSAVTPKNINEFKKKVL
                     TGIYLMMWSHYSSTVSNLMNSTLVELFQRDLQVSSPQKMSDTLFDASLEALSQYCSFA
                     YQNRNERIYDNLIRRLGKTVQADIHALRHSREKQETSFYDIYKGIMRTLGLNSVF"
BASE COUNT         2924 a         1841 c         1742 g         2802 t
ORIGIN      
        1 agaaatggat aacttgtttt atcaacgcat accagataat ctgtagataa agaagctatc
       61 atatctcctt ctgaaggttc ttttacataa tccactacat aagataatcc cgagcaaccc
      121 gtctttttta cagacaacct tacccccttg tttcctgggt ttttctccag ataggtgacc
      181 aggtgcttta ttgcagattc actgaaagtg atattggggt tcgtactgct cgcttgttgt
      241 atttctacac tcattgcttt acctctcaaa tttcttacgc atcaaagtca atgcttcttt
      301 atatatgtcc gctacctgga ctgctacagg atactgatta tttgttattt gtaatacatt
      361 aattagaatc tgaaaatcta ttaatggaac tgattccaaa gtcttacctt caatctggca
      421 acatatccat tccattgccg caataacata agggttacca ttcgttttaa atctagcttt
      481 ggatatgagt ttatctccgt cacattttat ataaaaatgt actgtgttca gttgattaat
      541 cggttgactt tgtacaccta ttacaaaagg atcatttaaa tccagcttac caatatgttg
      601 tggctgaaaa aaataatcct gtacaatttt attatacatc ataacggcga taattcgtgc
      661 agccgactca cttgtctgca aattatatca attgccttct ttatttgcac ctcagaagtg
      721 aagcgtccta tggataaacg aatagttgac tgcgcctctg tatcagtcaa accaattgcc
      781 cttaagacat aagatggttg aatactagca gaagaacagg cagaagtagt tgatatcgct
      841 aactcactta aagcaaacag caatgaatct ccatttaatc caacaaaact cacattcaga
      901 tttccagcga tacgcctgtg ctcatgtcca ttcagtttaa tagcaggtaa atgtcgtatt
      961 ccgtcccata gttgtttgcg taaatttaga atacgcgtct gttcaggaat tctatcagtc
     1021 tctgcaatag caaaagcctc acccatgccg acaatttgat gggtaggtaa tgttcctgag
     1081 cgcaatccac cctcatgccc gccaccgtaa cttaaaggct gtaaacgaat ccttggctta
     1141 tgcctgacat acagagcccc tacaccttta ggcccataat ttttatgagc agagaatgac
     1201 atcaaatcaa ctgaaagctg gctcaaatcg attggcaatt taccagcact ttgagccgcg
     1261 tcaacatgaa atataattcc cctgtttcgt aatagctcac caatagatgc aatatcttgg
     1321 atgacaccaa tttcattatt aacatgcatg acagaaacca gaatagtatc tggcctaaga
     1381 gcagattcca gttttccaag atccaacaaa ccatctgatt ctggatgaag gtaggtaact
     1441 tgaaaacctt ctttttcaag ttggtgaaaa ctgtcaagca cagctttgtg ttcagtgctc
     1501 atagtaacca gatgcattcc tttgttttta taaaaccggg cagctcctaa aattgccaaa
     1561 ttatccgctt ctgtagctcc tgatgtaaac actatttctt ggggtgaggc attaatagtt
     1621 tctgcaattt gagagcgtgc ctgctcaact gccatagagg cagctctccc atactcatga
     1681 gttgctgatg caggattacc aaaatcccct tcagggccta aatattttat catttgctca
     1741 acaacgcgag gatcaacggg tgttgttgcc atataatcaa aataaatcgg ttttattgcc
     1801 aattctattg ctcctattgt ctttccttgc ccactccaat gatttctgaa cctggctaag
     1861 catacctctt aaaatactaa cttccatttt ttcaaggttg attctattaa acaatcgcct
     1921 cactctttgc attaatctcc ttggattaga gggctttaaa aaattaattt caataaaaac
     1981 ttctttcaga tgctcataaa attgctctat ttcatcagct gtagccggtt cttcattgcg
     2041 cagagctact tgagcactcg gtgataataa cttcatacga agttcataag cgataatttg
     2101 tactgcctga gccaaattta atgaactgta ctcaggattg cttggaatat tgatatggta
     2161 gtgacatttt aacagctctt cattagttag gcccgcgtgt tctctaccaa atacaatggc
     2221 cacttgagta ttatctgatt gttgatttat caattctgca caggaagctg gaattaatcc
     2281 aggtaatgac agtccacggg gtctggcact cgttcctaaa atcaactgac atccaattaa
     2341 agcttcgtcc aaagtatttg taacaactgc tgcatccaga acatcatcag ctccagcagc
     2401 catttctttt gctttgagat caggaaatga ctttggatta accaggtaca gagtacttag
     2461 ccccatggtt ttcatggctc ttgcagtaga gcctatattg ccaggatggg aagttgaaac
     2521 taaaacgatt cgaatagaac ttaacttcat aaaaaattat tccaaataca atagataact
     2581 atatcatagg atttattaat tgaacttctc tttgtttaaa aaaaatgtgc tagaattgct
     2641 gattttatag ctaaagagta tatccatgga accactttta aatattgctg tcaatgctgc
     2701 gcgacaggct ggtgaaatca ttaatcgtta tgtagagcaa gttgatcgtt taaaaataac
     2761 tcccaaaaac agccatgaat attttagtga agtggacatt aaagcggagc aagttatcat
     2821 taatacgata cataaagcct atccagagca tggaattctt gccgaagaaa gcggtattca
     2881 gcaaagcgat agtgatacag tctggatcat tgatccattg gatggcacct caaattatct
     2941 tcatggattc cctttttatt ctgtatccat agccctgaaa attaaaaatc gtctggagca
     3001 tggtgttatt tacgatcccc tgcgacatga gtgttttgct gcaagcagag gacgcggggc
     3061 aaggctaaac gatagaagaa tccgtgtgtc caagcaaact caactcaatg cctctctgtt
     3121 aggtacaggt tttcattttc gtgatgcaac tcttgcccag cgatatttac caaccttcga
     3181 agcgttgatt gggaaatgcg ctggagttag aagaacaggt tctgctgctc tcgacttggc
     3241 ttatgttgca agcggtcgtc tggatggctt ctgggagttt gggttgcgcc catgggatat
     3301 tgctgcggga gccctgctgg ttcgagaggc tgggggatta ataagtgatg tccagggtgg
     3361 agaagatttc ctgaattatg gcgacgtagt agcaggaact cctaaagtat ttaagtcact
     3421 tttgcaaacc atatcacccg ttttaaaata agttgtcaat ttaagctcat ttaagacgtt
     3481 gatttaggct tatcgatttt aaattatttt cgaatatccg ctctctcgaa gctgagaaag
     3541 cggataaata ttcctctcca ctcttatctt aatcctggct tcattcctaa aattactggc
     3601 aactcatctg ccatcgccgt gcctaaattt ttagttaggc ggtcaaagac attttgcttg
     3661 taagtatagt caactatatc agtaatttta attacctctc gcgccaactg cccgctactg
     3721 gcataaccat caattaaacc attagccaac gcttgctcgc cagtccaaaa taacccggaa
     3781 aaagtctcat catcaacatg taatctattt cctcttcctt ctttgacacg agtaataaat
     3841 tgcttatgaa ctatatcaag catggtttgt agcttttcct tctgaaattc agtcgttggg
     3901 gaaaaagggt ccaagaaagc tttattgact cctgaagttt gtaatctgcg tgaaatacca
     3961 agcttctgca atgcatcaac aaaaccaaag ccattatata aaacacctat tgagccgacc
     4021 atgcttgcag gacttgcata gatttcatct gctccaactg caacataata ggcagcagaa
     4081 gcacacatat cgacacaaac agcgtaagtt ttaatgtttg gttttaggct tttgtaatat
     4141 tgtaaaacat tataaatata ttccgcctgt acaggactgc caccagggct atttatccgt
     4201 acaattaatg cttttaatcc tttacttcta taagctttct caattccttt ggtaaaatta
     4261 tcagcacttg ctgaactgga atcagcaatt tcaccgttaa tatcaattag cccaatatgc
     4321 tctttatcct tagatgcttc cgtggttttg gcgactgaaa caatcttata agatgaataa
     4381 actaatacga ccaggataat agttcttata atccatttcc atcgacgttt tcttttcctt
     4441 tcttggagat agtcaattac cagttgatta agcaaagctt gtgaatccaa attagaatta
     4501 gaagatgact cattattcat aaaaaactct tgaaaaaatg taaataatac ccattttatg
     4561 gcatgtaaac tagaattaat accctaaaat aactagaaag gtgttcctat gcgaatggac
     4621 aaactaacat caaagtttca aatggctctt gcagatgccc aatccctggc tttaggtaga
     4681 gacaatggat ttatagaacc agaacattta atgaaagcct tacttgatca gcaaggcgga
     4741 agttgcagac ctcttctcag taaagcgggt gttaatatac cactattaag aactttaatt
     4801 gaccaagctc tggataaact acctaaagta tcaggcaccg gaggagatat tcatatatct
     4861 aatgctttga atagactact taacctgact gacaaactat cgcaacaaag aaaagataac
     4921 tttatttcaa gcgagctttt tgtattagca gcgataaatg aggatagtaa ccttgcaaaa
     4981 atactcaaac aagccggggg tgacaacaaa gcaattgaaa aagcgattga tgagttaaga
     5041 ggtggagaaa cggttaatga tccaaatgca gaggaacagc gacaagccct tgaaaaatat
     5101 acactggatc taactgaacg tgccgagcaa ggaaaattgg atccagtgat tggacgagat
     5161 gatgaaatca gacgcaccat ccaggtattg caaagaagaa ctaaaaataa ccctgtatta
     5221 ataggtgaac ctggtgttgg taaaacagcc atagtagaag gtttggcaca acgaattatt
     5281 aatggtgaag tacctgaagg attaaagaat aaacgcttac tggcactgga catgggtgcc
     5341 ttaatcgcag gtgcaaaata tcgtggagag tttgaagaac gtttaaaagg agtgcttaat
     5401 gatttggcaa aacaagaggg ccaaatcata ttatttattg atgaattaca cactatggtt
     5461 ggcgctggca aggctgaagg agctatggat gccgggaata tgctcaagcc agcattggct
     5521 agaggtgaat tgcattgcat tggggctacg acattagacg aataccgaca atacattgaa
     5581 aaggatgctg ctcttgaacg acgctttcaa aaagtactgg tcgatgaacc cagtgtggaa
     5641 gacactatcg ctatattaag gggacttaaa gaacgttatg aagttcacca tggagttgaa
     5701 attacagatc ccgctttggt tgctgccgca atgctatccc atcgttacat cagcgaccgt
     5761 cagctacctg ataaagccat tgatttaatt gatgaagccg ccagcttaat ccggatggaa
     5821 attgattcaa aaccagaaag tatggataaa ctggaacgac gtttgattca gctaaaaatc
     5881 gagcgtgaag cattgaaaaa ggaaaatgac gaagcgtcta aaaaaagact tgttgattta
     5941 caaaaaagta tcgatgagtt agagcaaaat tattcagact tagaagaaat ttggaaagct
     6001 gaaaaagcga ccatgcaagg atctactcaa atcaaagaag cacttgaaca agccaaactg
     6061 gaaatggaaa ctgcaaggag agcaggagat ttaagtcgca tgtccgaatt acaatatggg
     6121 cgtatacccg agctggaaaa aagattaagc caggtctctt ccgtcgatgc tatggaaaca
     6181 aagttggtac gcaataaagt caccgaagat gagatcgcag aagttgtttc aaaatggaca
     6241 ggaatcccag tatctaaaat gatggaagga gaaaaggaaa aactgctgaa aatggaagaa
     6301 gctttgcaca gtagactcat tggccagaat gaagcggtgg atgctgtttc taatgccata
     6361 agacggtcac gcgcaggcct gtctgatcct aatcgtccaa ttggttcatt tctttttcta
     6421 gggccaactg gtgtgggaaa aactgaacta tgtaaagcac ttgcttcttt tctttttgat
     6481 acggaagagg cgatggtgcg cattgatatg tctgaattta tggaaaaaca ctctgttgcg
     6541 cgcttaatcg gagcgcctcc tggctatgta ggatatgagg agggtggtta tttaactgaa
     6601 gcagtcagac gcaggcccta ttctgttatc ttacttgatg aagtagaaaa agctcataca
     6661 gatgtattta atattctgct tcaagttatg gatgatggcc gtctaacaga tggtcaggga
     6721 cgtactgttg actttaggaa taccgttatt gttatgacct ctaatctggg atctcaactc
     6781 attcaggaaa tgagcagcaa atttaattac gatgagatta aggctgcggt aatggattta
     6841 gtcagccaac atttcagacc tgaatttatc aacagaattg atgaatcggt ggtattccat
     6901 tccttgacaa aagaacaaat tgcaaaaatt gcagcaatcc agattaacta tttacatcat
     6961 cggctgaaac aacaaaacat cactcttgaa gtcacaagtg aggctttatc tcatttggct
     7021 gaagcaggat ttgatccggt ctatggtgcc cgccctctaa aacgtactat tcagcaaaaa
     7081 ttagagaatc ccctagctca atcattattg actggaaaat ttaaatcagg ggataccatt
     7141 attgtatctt ataaagacgg agtaatggaa ttttccaagc aatgagtatg cttatcattg
     7201 gataacaacg caggagtcct tatccaggac tccaaaatta cgaggcaacg tttcaagacc
     7261 aagctgggta aacacatcaa acaaataaag acgtagtgcg agtctctgaa ttatcttcct
     7321 gagctttgtt tactgtttga acttttttag ctaaagccaa ttctgtttga agaataaatg
     7381 tattcattga tccttttcca ccaaaaaaat caaaattaag ctcaacttta ggattagctt
     7441 caaaatatgt tttaagaagc ctgattgcgt caagcatatc cttatcagtc aactcattgg
     7501 gatttatttc cacacccaga gcctggtgaa tgcaaaaccg taattgctcc tttacgccct
     7561 cgttttgtaa taacaaacaa taaccaccca aagcccgata tcttgcaaga ctgttattac
     7621 aatttttcat tgcttcaata aacgaaccac aatccatcgt gctaaaattt tcctgaatat
     7681 gtccggtata caacagatca ataatttttt cctgtaaaac gttcgattca atttgagctt
     7741 ttatttgctc ttccatcaat tccaaggata tggttttaaa atcggaacag gtttttggca
     7801 agagtttgcc taaatgagtc aaagcctctt caatttgatg ttgctcttct tcaatttgtt
     7861 ttaccaagga ttgaatgaaa tttatggcct tgaattgatt taaaacaaca gggttacgtc
     7921 ttttgtgttc atcaataatt tcttgtaaat aggtttcaaa attcttgtct gctgcaaaat
     7981 gaggatagct tttatagcgg ggcattttcc cctcatcctt ccctataatc aattgcatat
     8041 tatcacgaaa tgtttccaaa gcagttacaa tcgttgttac atcaagaatt ttcaagtcgt
     8101 ttattctata atttttctcc atcacctcag gtaaacctaa agcgagtctt attgcttgaa
     8161 acaatcgaca atcctttaca tcagatggcg gtttatattt aaaaaaaaat gatgggacgg
     8221 gaatgtaaga attaaagtta tcatactcct taatcagtct aaaatagcga tgcagcaaag
     8281 cacctaacaa aaacaggatg gcttgcttgt ttttttcctc attattttct tttaaaactt
     8341 ctcgcaattc attacttaat tgtttaaaaa catctctggc agtttctcta tctgttttgc
     8401 aaatcacaac cttatgggtc cgagctaata atgtttccaa agtattgcta attatttcac
     8461 cactcgatac cgcttcagat acaggaggat cagtaataat tttatacctg cctagcaagg
     8521 tagagaatac tgtctctaag ttacgagtat ttacacttaa tattttgaac tcgggcggag
     8581 taaaagtcat agtgtctcct gtctaaaaca cgctattaag tcctaaagta cgcataattc
     8641 ctttataaat atcatagaat gaagtttcct gtttctccct ggaatgacgt aaggcatgaa
     8701 tatctgcttg caccgtctta cctaaacggc gaattaagtt gtcataaatg cgttcattac
     8761 ggttttgata ggcaaatgaa caatattgac ttaaagcctc taaactggca tcaaacaaag
     8821 tgtcactcat cttttgtggg gagctcactt gcaaatctct ttgaaatagc tcaaccaaag
     8881 tgctattcat taaattcgaa acagtagagc tataatgtga ccacatcatt aaataaattc
     8941 cagttagtac cttttttttg aattcgttaa tattttttgg agttacagca gagtatcttg
     9001 tgacaccctt aagataagcg attaagtcat cgtcattctc gctatccagg ctataagcaa
     9061 ccaatttaat cgtatgagta tcttcaatgg ccgcagtatt gggtaatatc actgaaaaaa
     9121 aacaaaaaca gcctaaggat ggtatgctgc gtttctttaa ttgatctgtt aatttcaatg
     9181 cattttgctg aaattctgca aactctagca tggtttccct cccacaaggg cccacccgca
     9241 agaaccgcag cgagtaatac gcctttgatg atcaagactc ttttaatttt tatatcaacc
     9301 tacacataa
//
LOCUS       DAXLDT010000116         9264 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXLDT010000116 DAXLDT010000000
VERSION     DAXLDT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.116"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            40..1167
                     /gene="lpnE"
                     /locus_tag="ACF2SP_002259"
     CDS             40..1167
                     /gene="lpnE"
                     /locus_tag="ACF2SP_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LpnE"
                     /protein_id="HFD2360034.1"
                     /translation="MDMKKYILSLSLLTVINSSAIATTQEGEEAYNKGYYEKAFILLS
                     PEADKGDAKAQYLLGKMYYNAQGVTYNPEKTEQLLLASANQGNVDAQVLLAGFYWYLN
                     TPEGYKKAFEWYQKAADQNNADGQYGLGYMYDTGTGVPQNSDTAMVWYKKAAEQGNSN
                     AALAIGYNYDTGTGVKKDKTQALNWYAKAADLGNASAQYNLGLMYEQGDGVPKDYQKA
                     AEYFEKAANQGHAKSQLELGYLYDSGKLGKSDLQKAAFWYQKSADLGNANAQFNLADM
                     YFYGDGVGKSLEQSVYWMQKAAEQGYGKAQNQLGIYYRDGIGVAADPVKAYAWFTAAK
                     NNGFEKATSNASDLEKSMNPEDLSKARTLGQQYTDNYKAKK"
     gene            1545..2264
                     /locus_tag="ACF2SP_002260"
     CDS             1545..2264
                     /locus_tag="ACF2SP_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360035.1"
                     /translation="MTERKKTTVKNKKAPVKKSQLTVSLLIFIDLISILLSLMPGVAA
                     FSLTGLFLYYGFSKTWLVLICSPFVLLFSFILIIALIRISLPKLKPGRYKRELNKTMI
                     AWFCHFALSRAAKIVGLIHLLQSFNVIKYLYWRALGIKVSFRVMNSFDIDFVDCPLIS
                     IGKDVTLASQVSISCHSDVGNYLFLSPVVIEDNVFVGMTTKIGPGTTIKKGAWIGYGN
                     AFVNQVIEENAKIGNVRPVPE"
     gene            2407..3906
                     /locus_tag="ACF2SP_002261"
     CDS             2407..3906
                     /locus_tag="ACF2SP_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360036.1"
                     /translation="MKKIVYVLLFISSLTHANCVIYFNEQPLNKRLSDPLYSLLSNTE
                     ICPQSIQELRNSMRLRGLKEVIGMVANRGRNNPVEGSFSFFESVYGRLATDQLIAKGE
                     LFLGYFTRLKDGLIILDQKPRKGKLLIEAISWDAKKALYNFYELRGIEHGGVRWFYRG
                     NSKDAYKDNTWLYRISPEGENHFGSRMRCSACHNSGGPILKEQSLPHNDWWTNQRPLI
                     LLPNKPDGDVSQLLEQVSDASVFANDVITGMRHLSDSKKMKAFQGKLSLQEQLRPLFC
                     TTEINLQSSSDSSETVVSIPSAFWLNPLLGDINLTISAEDYNQLLKEFGTRFPETSLQ
                     DADHAWLTPVKAEADLNAIKQLIQQNIISKKFAQSVLMIDFTNPLFSSKRCELLKLIP
                     ESYQTNSKEWIEQFLINLRNNEEQFKGARQLADYMSKEQLDSKELMKKIKIYEYFLYQ
                     SISTRAGLRKHFQHLIELRKAVFRSELSQNPLGQILEPGFRVIFPETQQ"
     gene            complement(3969..5090)
                     /locus_tag="ACF2SP_002262"
     CDS             complement(3969..5090)
                     /locus_tag="ACF2SP_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360037.1"
                     /translation="MAAQRHLYIFTLIGLLLGVAVDILIRYNNTTAFIYSVVTIFGVL
                     FALTYNNVNFSRLIGTSFLLAFFLSIPLFPLKMDYSMKDYFHFFTFFVGFPFFIYVAH
                     CFHYAYHHDNTWRVSYSSLFAGVWNTIPLLFIALVFSSLANLLIVLGSFVFKTVGNNY
                     LWDLYFYNRDFKLISNITLFFMGLGVGQQNLNIIHNMRFLLLRIMYYLFPLLAAISIL
                     YFILYTFHSFSSSQEHINPLIVLIPLTTAGIIFFNAYFQDGTIKSDYPSWLKLSLRVY
                     RVILFLLALMMTYKILSNFSLDTNAFIYLLVAVLFSFTYAITAFLNENQEKQWIYMGN
                     IGTAIFFIVTLFLCNLPYIPVEFTIGGGNAINFITSTLS"
     gene            complement(5251..6336)
                     /locus_tag="ACF2SP_002263"
     CDS             complement(5251..6336)
                     /locus_tag="ACF2SP_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HFD2360038.1"
                     /translation="MKNLYIDPDITKASTISSEFYTSTEWFEITKEKIFSKTWQFCIS
                     TEELTTNRQLIPFTLLPGFLEEPLLFTKDESGTIRCLSNVCTHRGKILIEEPCVAHKI
                     KCSYHGRRFNFCGELIHMPEFELTKDFPSSNDNLSQISTGILEPFLFASLSPIVPFHE
                     VFAEIKERLFWLPMEKMRLDKEKCKDYVVKSHWALYCENYLEALHIPFVHPSLRKVID
                     CNTYTTELFRYCNLQLALASPDEVSFDLPKGAQDYGKQVAAYYFWIFPNTMLNFYPWG
                     CSVNVIKPLSPELTQVSFLSFVLDESKLGKGAGGALDQVEQEDERVVESVQRGIKSRY
                     YDMGRFSPTKEQGTHHFQRLLCEFLND"
     gene            6544..7518
                     /locus_tag="ACF2SP_002264"
     CDS             6544..7518
                     /locus_tag="ACF2SP_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HFD2360039.1"
                     /translation="MRYSRFLCLLNGMLLSSNVAAFSPSAPIFSPYVDLTINTHWDSQ
                     SQDMEPMDLISPAQKLGIKAYHLAFITDSGQCQPAWGAQQNYSVAKGWGKKQFDTLSR
                     EGVKLTVSFGGASGTDISYHCDKNQLINRFNQVVNQYHANVLDFDIENGTANIPNLLQ
                     SLKLFQKEHPDVLLSFTLPVMPEGLTSVGKEIITSAATLGLHFNVNIMAMDYGPAYNG
                     DMGDYAISAATNLHQFLQEIYPDKKPEALWQMIEVTPMIGVNDVNTEQFTLSNAAQLK
                     QFAQKNLLGGLSMWSFNRDKPCADKWASPVCSGNNLQSHDYEFVKYFQ"
     gene            complement(7754..>9264)
                     /locus_tag="ACF2SP_002265"
     CDS             complement(7754..>9264)
                     /locus_tag="ACF2SP_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360040.1"
                     /translation="QETVRAIYGEQFYEGRLDKLPFISVFFVIEKNKRMQFAIDHQDK
                     IKDAQTLAHVLKELPEEKKLDFAGKCKDIIADSKSLNAVLENLPLDQRLDFASQCKKK
                     IRDALDLANVLTKLPENKKLKFATTCEYKIGDATGLASVLFRLPEGERFQFAIDCEDR
                     VRDSAELSEVSLHLPDEQRLEFIMRSEDKINDASDIRSVLLVLPYYQRLKFASRHENK
                     IKNSYDLGSFLYGLFHDDKIDFVLKHENKIKDAQGLAYVLNNLPQENKINFARKHEDK
                     IKDVAGVVSVLIELPQDEKISFAIKHEDKINNATELASVVYELPLTNRLDFAIKHENK
                     IKDALEIAMVKDVLPKDKRLYFAARNEDRIKANEKQLSLSNNAKIHMSIVKIPADERK
                     ENILAVYRVLKNDDFLRNQDGTVSTIIKQIKEIVREIDSSKEVNITNAIMEIKKMIVN
                     NKDNNHSENANYIINAFAKPSCCDFRKIREALSTNPAIYEIMNRKEFIAKNE"
BASE COUNT         2953 a         1557 c         1813 g         2941 t
ORIGIN      
        1 tactttaaag cgatagctct taaaaataag gtatatatca tggacatgaa aaaatatatt
       61 ttatctcttt ctctactgac ggtgattaac tcttcagcta tcgctacgac tcaggaaggt
      121 gaggaggcct ataacaaggg gtattacgaa aaagcattta ttttgttgtc tcctgaagcg
      181 gataaaggag atgccaaggc tcaatatttg ctgggtaaga tgtattataa tgcccaaggt
      241 gtaacttata atccggaaaa aacagagcaa ttattacttg cctcagcgaa tcaaggcaat
      301 gtcgatgccc aggtattgtt agctggtttc tattggtacc ttaatacacc tgagggctat
      361 aaaaaggcgt ttgaatggta tcagaaagca gcggatcaaa ataatgcaga tggtcaatat
      421 ggcttgggct atatgtatga tacaggaact ggtgtacctc agaattccga tacagccatg
      481 gtatggtata aaaaagcagc agagcaaggc aactctaatg cggctttggc tattggttat
      541 aattatgata ccggtacagg ggtgaaaaaa gataagactc aggctctcaa ctggtatgca
      601 aaagcagctg acttaggtaa tgccagcgca caatataatc ttggtttgat gtatgagcaa
      661 ggggatggtg ttcctaaaga ttatcagaaa gcagcagagt atttcgagaa agcagcgaat
      721 caaggtcatg ccaaatcgca attggagtta ggttatttat atgattctgg aaaattaggt
      781 aaatcggatc tgcaaaaggc tgctttttgg tatcaaaaaa gcgcagatct ggggaatgcc
      841 aatgcgcaat ttaatttagc tgatatgtat ttttacggtg atggggtcgg taaaagcctt
      901 gagcaatccg tatactggat gcaaaaagca gctgagcaag ggtatggtaa ggctcaaaat
      961 caattaggta tctattacag agacggtatt ggtgttgctg ctgatcctgt aaaagcttat
     1021 gcctggttta cagcagccaa gaataatggc tttgaaaaag cgacgagcaa tgccagtgat
     1081 cttgagaaat ctatgaaccc agaggatctg agtaaggcga gaactttagg acaacaatat
     1141 acggataatt acaaggccaa aaaataaatg cctgttacct gttttcaaga gctagggata
     1201 ctgcatttgt gggctatggg agtgtgcgag agaattctcg catacactcg cccaaaaata
     1261 taaaattaat ttcaccagaa taaagagacc tctttcaatt taagaaagta tcttccaatc
     1321 ttttgcttgt ttttgttagt atacctccat atttaaataa aacagacagg acttacgaaa
     1381 aaccccaatc tctttgttaa aaaatgtttt taattcggtc atttagttta tctaaactcc
     1441 ctcattaaaa acattatttg ccttgacctt gaggtttttc gtaagtcctg acagactcaa
     1501 aagttggcaa atgagttgtc tattattttc aaggaaatca caggatgacg gagcgcaaga
     1561 aaacgacagt caagaataaa aaagcacctg tcaaaaaaag tcagctgact gtttctttat
     1621 tgatttttat tgatttgatt tctatattgc tcagtttaat gcctggcgtg gccgcattta
     1681 gcttaaccgg tttgtttttg tattatggat tttcaaaaac ctggcttgtt ttaatttgct
     1741 caccgtttgt attacttttt tcttttattc ttatcattgc acttatcagg ataagccttc
     1801 caaaacttaa gccaggtcgc tataaacgag agttaaataa gaccatgatc gcctggtttt
     1861 gtcattttgc actctctcgt gcagcgaaaa ttgttggtct gattcattta ttacaatcct
     1921 ttaatgttat taaatatttg tattggcgtg cattgggaat taaagtgtca tttcgcgtta
     1981 tgaactcatt tgatatcgat tttgtggatt gccctctcat ttcgattgga aaggatgtta
     2041 cccttgccag tcaagtctct atcagttgtc attctgatgt aggaaattat ctttttttat
     2101 cgcctgtggt gatagaggat aatgtttttg ttggaatgac gactaaaatt ggcccaggaa
     2161 caacaattaa aaaaggggcc tggataggtt atgggaatgc ttttgttaac caggttattg
     2221 aggaaaatgc aaaaattggc aatgttagac ctgttcctga atagaatgaa atgttttatc
     2281 ttaagtttgg attaataaaa atcagaattt agtgcattgt tttcccggtt aacttaatga
     2341 atccagattg ctgcaatcag gctttgaagc cgctgattat ttctttgatt aaacggatgt
     2401 tccagaatga aaaaaattgt ttacgtcttg ttatttattt cttcgctgac tcatgccaat
     2461 tgtgtgatct attttaacga acaaccgctt aataaaaggt tatctgatcc tttgtattct
     2521 ctgttgtcca atactgaaat ttgcccgcaa tcgattcagg agttaagaaa ttccatgaga
     2581 ttgagaggat taaaagaagt catagggatg gttgctaata gaggaagaaa taatcctgtt
     2641 gaaggcagtt tcagtttttt tgaatcagtg tacggtcgat tagcaactga ccaattgatt
     2701 gcaaaggggg agttatttct ggggtacttt actcgtttaa aagatggcct aattattttg
     2761 gatcaaaaac ccaggaaagg taaattattg attgaagcaa tcagctggga tgctaaaaag
     2821 gcgctttata atttttatga attgcgagga atagaacatg gaggcgtacg ttggttttat
     2881 cgaggaaact cgaaggatgc ttataaagat aacacctggt tatatagaat aagtccagag
     2941 ggcgaaaacc attttggcag tcgtatgcga tgttcagctt gccacaactc cggcgggccg
     3001 atccttaagg aacagtctct tcctcataat gattggtgga ctaatcaacg tccgcttatt
     3061 ttgctcccca ataagcccga tggagatgtg agccaattat tggaacaagt cagtgatgcc
     3121 agtgtatttg caaatgacgt gataacagga atgcgacatt tgagcgatag taaaaaaatg
     3181 aaggcatttc aagggaaact gtcattgcag gagcagttac ggcctttatt ttgtactacc
     3241 gaaataaatc ttcagtccag ttctgactca tcagaaaccg ttgtatccat tccatcagcg
     3301 ttttggctta atccactatt aggcgacatt aacctgacaa tttcagctga agattataat
     3361 caattattga aagaatttgg cactcgtttc ccagaaacgt ctttacaaga cgctgatcat
     3421 gcctggctta cacctgtcaa ggcggaggct gatctcaacg cgattaagca acttatccaa
     3481 cagaatataa ttagcaaaaa atttgcacaa tccgtgctga tgatcgattt taccaatcct
     3541 ttgttttcat caaaacgatg tgagttatta aaattaatac cggaatctta tcaaacgaac
     3601 agcaaagaat ggattgagca atttctgatt aatttaagaa acaatgagga gcaatttaaa
     3661 ggggcgcgtc agctagctga ttatatgagt aaagagcaat tggattcaaa agagctgatg
     3721 aaaaaaatca aaatttatga atattttcta taccagtcta tatcaaccag agcaggatta
     3781 aggaagcatt ttcagcattt aatagagctg cgaaaagcgg tctttcgtag tgaactgtca
     3841 caaaatccct tagggcaaat tttagagcca gggtttcgag taattttccc tgaaactcag
     3901 cagtagacaa gaagaggtct attggctctg cgcatgacaa ataactgtca tgcgctgagt
     3961 attattttct acgaaagagt tgaggtgatg aaattgattg catttcctcc accaattgtg
     4021 aactcaacag gaatataggg cagattacag agaaacaggg tgacaatgaa aaatattgct
     4081 gtacctatat ttcccatata aatccattgc ttttcctggt tttcattcaa aaatgctgta
     4141 atggcataag tgaaactgaa tagtacggca accagtaagt aaatgaaagc atttgtatct
     4201 agtgagaagt tggaaaggat cttataagtc atcattaaag ccagaaggaa cagaatgact
     4261 cgatagactc ttaaagataa ttttaaccag gaaggatagt ctgatttaat agttccatcc
     4321 tgaaaatagg cattgaaaaa tatgatacct gctgtagtca gtggaattag tactattaac
     4381 gggttgatgt gttcctggct gctcgaaaaa gaatgaaatg tatataatat aaaatagagg
     4441 atacttattg ctgccaagag gggaaacagg taatacatta ttctaagtaa taaaaatcgc
     4501 atgttatgaa tgatattgag attctgttga ccaacaccca aacccataaa aaataaggtt
     4561 atattactga ttaatttaaa atcacgatta taaaaataca aatcccataa ataattattt
     4621 ccaacggttt taaatacaaa agatcccaat acaataagta aattggccaa tgaggaaaag
     4681 acgagggcaa taaacaaaag aggaattgta ttccatactc cagcaaataa actgctgtaa
     4741 cttacccgcc aggtgttgtc atgatgataa gcgtaatgga aacaatgagc gacgtagata
     4801 aaaaatggaa agccaacaaa aaaagtaaaa aaatggaaat agtctttcat tgagtagtcc
     4861 attttcaacg gaaacaatgg tattgataga aagaaagcga gcaggaagct ggttccaatc
     4921 agacgcgaaa aattgacgtt gttataagta agggcaaata aaacaccaaa gatagtgaca
     4981 acactgtata taaatgcagt cgtgttgtta tagcgtatca gtatgtccac tgctacccct
     5041 agaagtaatc caatcagggt aaaaatataa aggtgtcttt gagcagccat cctgtctttc
     5101 tccacaaggg ggttatattc atgatttagg gtttggtaga atgccctaat cgttatctaa
     5161 aaataattct aactcagtta cttaattgat ctaaatcgtt tattaaaaat attttttcgt
     5221 tccaagtctg agcatgtgac agatctaatt ttaatcatta agaaactcac aaagtagtct
     5281 ttgaaaatga tgagtgccct gttcttttgt tggggagaaa cgtcccatat cataatagcg
     5341 agattttatc cccctttgca cagactccac cactctttca tcttcttgtt caacctggtc
     5401 aagtgctcca ccagcgcctt tcccaagttt tgattcatca agaacaaaag ataaaaagga
     5461 aacctgagtt aactcaggac taagcggttt gattacattc acagaacaac cccagggata
     5521 aaaattaagc atcgtattgg ggaatatcca aaagtaatag gcagcaacct gtttgccata
     5581 atcttgtgcc cctttgggta aatcaaagct gacttcatca gggcttgcca aagccaattg
     5641 cagattacag tagcggaaga gctctgtggt gtaggtgttg caatcgatta cttttcttag
     5701 cgagggatgt acaaaaggga tatgtaacgc ttccaggtaa ttttcacagt ataaggccca
     5761 atgcgattta actacataat ctttgcattt ttctttatcc aggcgcattt tttccatagg
     5821 cagccaaaac aatctttctt taatttctgc aaatacttca tggaatggga cgatggggga
     5881 aagtgaagca aagagaaaag gttcaagaat acccgttgaa atttgactta ggttatcatt
     5941 gctcgatggg aaatcttttg tgagttcaaa ttcaggcata tgaattaatt cgccacaaaa
     6001 attaaaccga cggccatgat aagagcactt gattttatga gctacgcaag gttcttcgat
     6061 caggatttta cctcgatggg tacatacatt acttaaacaa cgaattgtac ccgattcatc
     6121 ttttgtaaac agcaagggtt cttcaagaaa tccaggcaac agggtaaaag gaattaattg
     6181 tctattggtt gtcagctctt cggtactgat acaaaattgc caggttttac taaatatctt
     6241 ttcttttgta atttcaaacc attctgttga tgtataaaat tcagaagaga ttgtggaggc
     6301 ttttgtaata tcaggatcta tatagagatt cttcatgaac atttccttaa tgtaaagaga
     6361 aaaacctgca aaaagagaat aaacatttta accatttctt tggatgaagc gatgattaag
     6421 gtttggtaaa aggtagtgca aacctttgtg ccctgtcaac ttgtttattt gataacaaaa
     6481 tgattgatta attatcctaa tgatgcaaaa ataaacacag tttgggtttt aaggaactat
     6541 tgaatgagat atagccggtt tttatgttta ttaaatggca tgttattatc cagtaatgtt
     6601 gcagcgtttt caccctctgc ccctatattc agcccgtatg ttgatttaac aattaatact
     6661 cattgggact cgcaatcaca ggatatggag ccaatggatt tgatttctcc ggcacaaaaa
     6721 ctggggatta aagcttatca tttggctttt attacagaca gtggtcaatg ccaacctgcc
     6781 tggggagccc agcaaaatta cagtgtggca aagggatggg gtaaaaaaca gttcgatacg
     6841 ctatcaagag agggtgtgaa actcactgtt tcatttggcg gagcgagtgg tactgatatt
     6901 tcctatcatt gtgataaaaa tcaattaatt aataggttta atcaagtggt taatcaatat
     6961 catgcgaatg tcttagattt tgatatagaa aatggaacag ccaatattcc taatttgtta
     7021 cagtctttga aattattcca aaaggagcat ccagatgtct tgttaagttt tactcttcct
     7081 gtgatgcctg agggtttaac ttctgttgga aaagagataa tcacttcagc ggctactcta
     7141 ggccttcact ttaatgtgaa cataatggct atggattatg ggccggctta taatggggac
     7201 atgggggatt atgctatatc tgccgcaacg aatctccatc agtttttgca ggaaatttat
     7261 ccggataaaa aaccggaggc tttatggcaa atgattgaag taacgccaat gataggtgtg
     7321 aatgacgtaa atacagagca atttacctta tctaacgcag cccaattaaa acaatttgca
     7381 cagaaaaatt tgctgggggg attgtcaatg tggtctttca atagggataa accttgtgct
     7441 gataaatggg caagtcctgt atgtagtggt aataatttgc agagtcatga ttatgaattt
     7501 gtgaagtatt ttcagtaatg gaattaatta aggatttcaa taagaaaaat aaaaacttaa
     7561 ttttaaatta cccatgttaa acgcagaagt ttgaggttta aatattaaag gggtagtgtt
     7621 tagctcttaa ttatagagaa gtgtaaatgg atatgcttaa tctttatagt gaatttgatt
     7681 ttgcaaaaaa catgctatat ctataggttt gtctggattt ggtgaagcgg cattttggtc
     7741 taactgaatt agtctattca tttttcgcaa taaactcttt cctattcatt atttcataaa
     7801 ttgcggggtt tgttgacaaa gcttcgcgga tttttcgaaa atcacaacaa cttggtttag
     7861 caaatgcgtt gataatgtaa ttagcattct cgctgtgatt attgtctttg ttgttaacta
     7921 tcattttttt gatttccata atggcattgg ttatatttac ttcttttgac gagtcaatct
     7981 ctctcacaat ttcttttatt tgtttgataa tagttgatac tgtgccatct tgatttctca
     8041 aaaaatcatc attttttaat accctgtaga ctgccaaaat gttttctttt ctttcatcgg
     8101 caggtatctt aacaatactc atatggattt ttgcgttatt ggataaagac aattgttttt
     8161 cattagcttt aattctatct tcatttctag ctgcaaagta tagtctttta tctttaggca
     8221 gcacatcttt aaccattgct atctccaggg cgtctttaat tttattctca tgtttgattg
     8281 caaaatcgag cctattagtc aaaggtaatt catatacaac tgaagcgagc tctgtagcat
     8341 tgttaatttt atcttcatgc ttaattgcaa aacttatttt ttcatcttgc ggtagttcaa
     8401 tcaaaacaga gacaacccct gcgacatcct taattttatc ttcgtgtttg cgtgcaaaat
     8461 ttattttatt ttcttgtggc aaattattca aaacataagc aagcccttga gcatccttaa
     8521 ttttattttc atgtttaagt acaaaatcta ttttgtcatc atgaaacaaa ccatataaaa
     8581 aagatcctaa gtcgtagcta tttttaattt tattctcatg tctgcttgca aacttgagtc
     8641 tttgataata aggtaacact aatagaacag atctaatgtc actggcatcg ttaattttgt
     8701 cttctgatct cattataaat tcgagccgtt gctcatcagg taaatggagt gatacttcag
     8761 aaagttcagc actatctcta actctatctt cacaatcgat tgcaaattga aacctttcac
     8821 cttcaggtaa acggaataaa accgatgcaa gtcctgtagc gtctccaatt ttatattcac
     8881 aagtggttgc aaacttaagc tttttgtttt caggtagctt agttaataca ttagcaagat
     8941 caagcgcatc cctaattttc tttttacact ggcttgcaaa atctaacctt tgatcgagag
     9001 gtaaattctc caacacagcg ttaaggcttt tgctatcagc gattatatct ttacatttcc
     9061 ctgcaaaatc cagttttttt tcttcaggca gttcctttaa gacatgagcc aatgtttgcg
     9121 catctttgat tttatcttga tggtcaatgg caaattgcat tctcttattt ttttctatca
     9181 caaaaaaaac tgaaataaag ggtagtttgt ctaatcttcc ctcgtaaaat tgttcgccat
     9241 agatagcacg cactgtctcc tggc
//
LOCUS       DAXLDT010000117         9171 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXLDT010000117 DAXLDT010000000
VERSION     DAXLDT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.117"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(124..1506)
                     /gene="mavV"
                     /locus_tag="ACF2SP_002266"
     CDS             complement(124..1506)
                     /gene="mavV"
                     /locus_tag="ACF2SP_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavV"
                     /protein_id="HFD2360041.1"
                     /translation="MKYAVTFCTTDQEVGSNPLWHSCLLLSKMDETSKLLEVVDNWGF
                     YGLPTTNRNNSWLDQLKIKMGLDVDLIGNHGMLRHEELRFLDQGCGLRGVTFELTKDD
                     FELLQKKCLQMATDQETAINEFVETQGIKGKPPEKTRIYPHEQFSRLIYATENIKAEQ
                     QGREARLKPFELRLSWGLFGPSLALSQNCKTQALSLLSYVLSKEQIERLTEYGEHPTV
                     PRRSGPMESIFLHSTGPLREHRKSSGDVVHYRDLKDPGVKLYWTVPPQELEALSEETI
                     KLLQIDGEYCEEVKAIVRKLQRLEWLLINSNLPEKYRPYKDELINKVVGCYKAFSFIE
                     PKQEEPKLGGWYGWALSLFSAPRSAEERTLQGKIKKAKSLFNSIYMAIVDNYQIDDNA
                     PMENENILPYEETGENRFENMVEAVASYLSRKDKINLCKIIGRTYCEDHVELEDNEGK
                     IALSPQPLAT"
     gene            complement(1913..5515)
                     /gene="enhC"
                     /locus_tag="ACF2SP_002267"
     CDS             complement(1913..5515)
                     /gene="enhC"
                     /locus_tag="ACF2SP_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhC"
                     /protein_id="HFD2360042.1"
                     /translation="MKSLVPWVCVLVASAGQQAFANDAFNAYRLGNYNEAAEPLIIKS
                     GQDAVAEYYLGRLYLYGYGQLKNNRVAMRYFTKSAEKGYLPAVLLMAKYSLLHEKNPE
                     EAARWFKQAAAVGDVSAQMFMAAAYLYGVGVKKNTDIARRYYIDAAKNGNPIAQYTLA
                     EYFLESKHAGNKKLGIIWLNKAAENGDPKALTELGRFYVAGQMVAKDTSKGVELLNKA
                     ASEQYAPAMLELGKLALMQNQYDEAIQWLNKAAKQSTEATLELAHIYLQDKSPVYDPK
                     TGFLLVLKAAQNGSVQAQKELANLYQKGIGVAADEELSKQWTLKAAENEKKNAEPPVL
                     AQAALWLSNDRTDKLEETAYQMNGIFSPWNNPSALRNNSYNQAPKLETISRQDIFKPQ
                     FELTQPNNIPISNYYDALISKQVEFQSNQWAYPTYRLNPFVEALERANSPVVQKLNLP
                     VPYADANYDDSEVGSFDLMDIWTGDWKRTVNYMSVVNYLYFRAILGNAQTQFEIGQLF
                     QYGIGLMQDDASAIIFYENAAEQKHLGAEYNLGILYLKRGKDENDYQQALNWLTDSAF
                     KGNKRAQYVLARILRQGIVGPDGKEYIKANDEQAMAMLYLSAANDYGPAEYELAEYLA
                     RDYNNGLSVDVRKQKIALIRKLYQGAVKNKVAEALLPLAFYNAMDDNRQKQEQAFKVA
                     EEQAETGNENAALLLGMLYDRGIGITADPAKAMYWYQQAGQNPVSQFILGTYITEGKG
                     VAQDKEKGLDLLKQSADSQFSYADFNLAVLKQQSGEDFLPNLIESYKLGNSHAGIVLA
                     DYYLSQSSVPEQMNQAKEIYQGLADKGDQYAQLKLAYMLQKGLGAAPDLAGAQHWYTA
                     SAEQGNPEAQYLLGQFYQLGETGEPDYNLAKHWYQKAAKHLSKADVALGFIYETVDDN
                     YAQALKAYENAAAKGDMLGAYNLALMYEYGKGVPVNYSKALSLFKEASEKGAPEAMSQ
                     LAGMYFYGLGQPRNEQQALVWYKKAASLGNGNALYALGLLSETGVGVKLDFPDALRYY
                     QDASDKGNEKAMLALARMYHYGLGVEKDHKRSASIYQKLAQKQNAYAQYQLGTYYIEG
                     TAGERLPEKGRELLQQASENGSLQARRVLQRLDAQTQGKVSYVEPVLFNNVPKFDGQS
                     ADMIYFDALNEWNHGEEVLSRMILQRLVTQYPNFIPAKRAYEQLNQAKLMNNLG"
     gene            complement(5542..6108)
                     /locus_tag="ACF2SP_002268"
     CDS             complement(5542..6108)
                     /locus_tag="ACF2SP_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase IV"
                     /protein_id="HFD2360043.1"
                     /translation="MNLKSRSSITAIVVILLTSALNISVFAAKSNKAATAEDKAEKKD
                     NGKFPLGCTAVGYQQNLKVLTLLPGKEGALQSMYFIYNTLPETVSLYQMHDKESLYSY
                     WLNHSIRGNSWAVLATGEKEVKFICTLGDGKKSYGKILDCADVIKVCEYVNVKFGLNN
                     KGNFWIVDSNTRNGAVSQVVHYGIIPGV"
     gene            complement(6105..6827)
                     /locus_tag="ACF2SP_002269"
     CDS             complement(6105..6827)
                     /locus_tag="ACF2SP_002269"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFD2360044.1"
                     /translation="MKKILLTTFMMSMVTVLNAGHFYGTALCAYPQYECIKVTGGQSW
                     EKLFPDPVQRDIVQRVNRTYNYLWSGREIAVPKNLANINIFDVSPFPLKIQSENEKQI
                     IVDQEKLAWGAYDAQGNLVWWGPISSGSDKCSDSNKVCRTLTGIFRVFSKENVRCTSD
                     VFPIGRGGAKMPYCMYFHKGFALHGSDDIPGVRASHGCVRMFIQDAKWLNENFVELSS
                     ERNNFMGTKVIVRPLNDSENKK"
     gene            7000..>9171
                     /locus_tag="ACF2SP_002270"
     CDS             7000..>9171
                     /locus_tag="ACF2SP_002270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFD2360045.1"
                     /translation="MSPLKSKIKTKSFNKLTKTQDITNTIERLFANSQDSIIVFNQQG
                     KIIAINEKAAALFKKSPDKLIDKPIWKLFKLNSFAIKRQFIQAKRCFLLANEGIAQQF
                     TWLESKAQKPVLAYHILFNKTEIEDTSIIFTKLTDILKSKTIEWILWSLTKISNHQEI
                     NEIIDQILQLTSDVFAADYTSVSFINNQQIARTISYYKFGKKEENISFSLINTPCAKV
                     IQKKAICYFNDVQKQFPNDKLLKKMKINTYLAGPITNPQDEVIGIMTILCKREVEFDT
                     LNKTWFGLFLSRINSEIERLINLRKLEFLASIPQQNPNPIIRILLNGDVVFTNDEGKI
                     ILNYWIKLFKGLPVELLKEAQRIQKSNQNTRIEMEIEDKIYLFTLVWIDEFKQINIYG
                     TDITQLKNTQNAMLNLTRRDTLTQIANRQYFEEKLIEKIYEHHLKEKSLALLLIDLDN
                     FKIVNDTLGHPTGDKLLMAVTNRMTRCLRANDFIARLGGDEFIVLLDQCNTDTAVKVA
                     EKIINVLDKPFQFDDYHMTITASIGIAIYPETSEIARDLLKYADIAMYQSKKTGKNKC
                     TVFSKNLHYKENQRNEILKKDIELAALKRELYIDYQPQIDLSDNTIVGIEALLRWSHP
                     TLGLILPTEFIPIAEQTGCIHIISQWFIEQTLLDYSQFQQLNSNIKLSINVSLSQLND
                     MRFTKILCDSLLQNNLSKSKIILDISERMIAPHFIQISKKLK"
BASE COUNT         2845 a         1975 c         1460 g         2891 t
ORIGIN      
        1 atttcataaa tcctttttta tttgatattg attcctaaaa agggttggtc tttatcagtc
       61 cctcaacacc aacccaagct ggaatcacaa aaagctcatg aactggaatc tattccacaa
      121 cctctacgtt gccaaaggtt gtggagataa agcaattttc ccctcattgt cttctagctc
      181 gacatgatcc tcgcaatagg tgcgtccgat aattttacaa agattaattt tatccttcct
      241 tgataagtaa gaagctactg cttccaccat attttcaaat ctgttttcgc cagtttcttc
      301 atagggaagg atattttcat tttccattgg agcattatcg tcaatttgat aattatccac
      361 aatggccata taaatgctgt taaataacga ttttgctttt ttaattttgc cttgtaaggt
      421 tctttcttcc gcacttcttg gtgctgaaaa taaagaaagc gcccagccat accaacctcc
      481 aagtttaggt tcttcttgct taggctcaat aaaagaaaaa gctttataac atccaactac
      541 cttattgatt aattcatctt tataaggccg atatttttca ggtaaattgc tattgattaa
      601 taaccactcc aggcgctgca gttttctgac aattgctttc acttcttcgc aatattcccc
      661 atctatttgc aataatttga ttgtctcttc agataatgct tcaagttctt gcggaggaac
      721 tgtccaataa agctttactc caggatcctt taaatcacga tagtgcacaa cgtctcctga
      781 agatttccta tgctcccgta aaggacccgt actatgtaaa aaaattgact ccattggccc
      841 actacgtctg ggcactgtgg gatgttcacc atattctgta aggcgttcaa tttgctcctt
      901 tgataataca taagacagca atgataaagc ttgtgtttta cagttttgtg aaagagctaa
      961 actaggccca aacaatcccc aggacaaacg caactcaaaa ggttttaagc gagcttcccg
     1021 cccttgttgt tccgctttaa tattttcagt cgcataaatc aatcttgaaa attgttcgtg
     1081 aggataaatt ctggtttttt caggtggctt ccccttaatg ccttgcgttt cgacaaactc
     1141 atttatcgct gtttcctgat cagttgccat ctgcaaacac tttttttgta ataactcaaa
     1201 atcgtctttg gttaactcaa aggtaacccc gcgcaaacca catccctgat cgagaaatct
     1261 gagctcttca tgccgcaaca ttccatgatt gccaatcaaa tcaacatcca gccccatttt
     1321 tattttcaat tgatctaacc aagaattatt acgattggtt gtcggtaatc cataaaatcc
     1381 ccaattatca acaacctcca gcaatttact ggtttcatcc atttttgaaa gcaatagaca
     1441 actgtgccac agtggattac tccctacctc ctgatctgta gtacaaaatg taacagcata
     1501 tttcatatca ccaccctcat cgatttgcat ctaattattt gaccttgatt aaatagacct
     1561 tttgcccaag ccaattcttt cattttattg ctgcttcgct aacatttctg cattgctcat
     1621 taactgcatt ataaactccg ctcctcaaaa cgtttgcacc ttgccctaaa aaataatcgg
     1681 cttaggcata aagcctaact aacaatttaa atctctaaaa gattattttt aaaattacaa
     1741 taaggcatgg cttcactttc gtaaatagcc aagatgagcg caacccttca tgtgtttaat
     1801 ataatcgagg caacaaatgc gggctgggcc ttgcggccca gaaaaatcgg ataattgatt
     1861 agtaaaattg aaacatcatc tgttaaatag ctcaaagagc cgagcaatga tcttaaccca
     1921 agttattcat taatttggct tgattcaact gctcataagc tcgctttgca gggatgaaat
     1981 tgggatattg cgtcaccaga cgttgcaaga tcattctgga caaaacctcc tccccatgat
     2041 tccattcatt taatgcatca aaataaatca tatcagcact ttgaccatca aatttgggaa
     2101 cattattaaa taacacgggc tcgacataac ttactttacc ctgggtttgt gcatctaatc
     2161 tttgcaatac tctacgggcc tgtaaacttc cgttttcact cgcttgttgt aatagctcac
     2221 ggcctttttc gggtagtcgt tcgcctgccg ttccctcaat ataataagta cctaattgat
     2281 attgagcata agcattttgc ttttgtgcca atttttgata aatactcgct gaccttttat
     2341 gatccttttc aacaccaaga ccataatgat acattcttgc caaagccagc attgcttttt
     2401 cattaccttt atcagatgca tcctggtaat accgtaaagc atctggaaaa tccaatttaa
     2461 caccaacacc agtttcggat aataaaccga gggcatacaa agcattccca ttacctaagc
     2521 ttgcagcctt tttataccag accaaagctt gttgttcgtt tcttggttgc cccaaaccat
     2581 aaaaatacat tccagccaat tgactcatcg cttcaggagc acctttctct gatgcttctt
     2641 taaacaaaga taacgctttg gagtaattga cgggcacgcc cttgccatac tcatacatta
     2701 aagccaaatt ataagcgcct agcatatctc cttttgctgc agcattttca taagctttta
     2761 gtgcttgagc ataattatca tctaccgttt cataaataaa acctaatgct acatcggctt
     2821 tagataaatg tttcgctgct ttttgatacc aatgtttggc caaattataa tcaggttctc
     2881 ctgtttcacc tagttgataa aattggccta ataagtattg tgcctcagga ttgccttgtt
     2941 ctgccgaagc agtataccaa tgttgcgcgc cagcaagatc cggtgcggca cccaatcctt
     3001 tttgtagcat ataagccaat tttaattgtg catattgatc gcctttatca gctaagccct
     3061 gataaatttc cttagcttga ttcatttgtt caggaacact gctttgagat aaataataat
     3121 ctgccaacac tatccccgca tgactattcc ctaatttata ggattcaatt aaattgggta
     3181 aaaagtcttc cccggattgt tgcttgagta ctgccagatt aaagtcggca taggaaaatt
     3241 gggagtctgc tgattgcttc agcaaatcca atcctttttc cttatcctgt gcgacccctt
     3301 ttccttctgt tatgtaagta cccaatataa actgacttac gggattttgt ccggcttgtt
     3361 gataccagta catggccttg gctggatcag ctgttattcc tataccacgg tcatatagca
     3421 taccgagtaa aagagcggcg ttctcattac ctgtttcagc ttgctcttcg gctactttaa
     3481 atgcttgttc ctgcttttgc ctgttatcat ccatcgcatt ataaaatgcc aatggcaaca
     3541 atgcctctgc cactttattc ttcacagcac cttggtataa ttttcttatt aaggcaattt
     3601 tttgttttct gacatctaca cttaaaccat tgttgtagtc tcttgccaaa tattctgcca
     3661 actcatattc tgccggccca taatcattgg cagctgaaag ataaagcatg gccatagctt
     3721 gctcatcatt tgctttaata tactcttttc catcaggacc aacgatcccc tggcgcaata
     3781 tccttgccaa tacgtattgt gctctcttat tccctttgaa tgcggaatca gtcagccaat
     3841 ttaatgcttg ttggtaatca ttttcgtctt taccgcgttt cagataaagt attcccaaat
     3901 tatattcagc acctaagtgc ttttgttctg cggcattctc ataaaaaata atagccgaag
     3961 catcatcttg cattaaacca atcccatatt gaaacaattg accaatttca aattgagttt
     4021 gggcatttcc aagtattgcc ctgaaataca aataattaac cacagacata taattgacag
     4081 tccgtttcca atctcctgtc caaatgtcca tcaaatcaaa actgccaact tcgctatcat
     4141 cataattcgc gtcggcataa ggaactggca agttgagctt ttgtacaaca ggactattgg
     4201 ctctctctaa agcttctaca aatgggttaa gtctgtaagt tggataagcc cattgatttg
     4261 attgaaactc cacttgttta ctgattaaag catcatagta attgcttata ggaatattat
     4321 taggctgagt caactcaaat tgcggtttga aaatatcctg acgagagatg gtttcaagct
     4381 taggtgcttg attatagcta ttatttctca aggcagacgg attattccat gggctaaata
     4441 taccattcat ctggtaagcc gtctcttcca atttatccgt tctgtcatta cttagccata
     4501 atgcagcttg ggcaagaaca ggaggctctg catttttctt ctcattttct gctgctttaa
     4561 gagtccattg tttagacaat tcctcatcag ccgctacgcc aatccctttt tgatataaat
     4621 tggctaattc tttttgggct tgaacagaac cattctgagc tgcttttagt accaataaaa
     4681 aacctgtttt tggatcataa actggacttt tatcttgtaa ataaatatgg gctaattcaa
     4741 gagtagcttc tgtactctgt ttcgcggcct tattaagcca ttgaatagcc tcatcgtatt
     4801 gattttgcat taaagccaat ttacccaact caagcatagc tggggcatat tgttctgaag
     4861 ctgctttatt taataattcg acgcccttgg aagtatcctt ggcaaccatt tgtccggcca
     4921 cataaaatct gcccaattct gttaaagctt ttggatctcc attttctgct gctttattca
     4981 gccaaatgat tcctaatttt ttattacccg catgcttact ctctaagaaa tactctgcta
     5041 aggtgtattg agcaatagga ttaccatttt ttgctgcatc aatataataa cgtctcgcga
     5101 tatctgtatt tttcttgacg ccaactccat ataaataagc tgccgccata aacatttgtg
     5161 cgcttacatc tcccaccgcg gctgcttgct taaaccaacg agcagcttct tccggatttt
     5221 tttcatggag caaactgtat ttggccataa gcaggacagc aggtaaatag cctttttccg
     5281 ctgatttggt aaagtagcgc atcgcaacac gattgttttt taactgtcca tatccataga
     5341 gataaagtcg ccctaaataa tattcggcaa cagcatcctg acctgattta atgattaggg
     5401 gttcagctgc ttcattataa ttgcctaatc tataagcatt gaacgcatca ttggcaaaag
     5461 cctgttggcc agctgacgcg actaaaacac acacccaagg tactagtgat ttcatgaaga
     5521 ttcccctctt ttcatggcca tttatacacc gggtataatg ccataatgca ccacttgact
     5581 aactgcccca tttctcgtat tgctgtctac tatccaaaaa ttacctttat tattcaaacc
     5641 aaatttgaca ttaacatatt cgcatacttt aatcacatca gcacaatcca gtatttttcc
     5701 ataggacttt ttgccatcac ccaaggtaca gatgaacttc acttcctttt cacctgtggc
     5761 caaaacagcc caggaattcc ctctgataga gtgatttagc caataggaat ataaactttc
     5821 cttatcatgc atctgatata agctaacggt ttctggcaat gtattataaa taaaatacat
     5881 cgactgcaag gccccttcct tccctggaag aagagtcaat acctttaaat tttgctgata
     5941 gccaacagca gtacaaccga gaggaaattt tccgttatct tttttctctg ccttatcttc
     6001 ggcggttgct gctttattgg atttggcggc aaataccgat atatttagtg cactagttaa
     6061 tagaatcaca acgattgcag ttatcgagct tcggcttttt aggttcattt tttattctca
     6121 ctatcattta aaggacgtac aatcacctta gtccccataa aattgttacg ctcactggac
     6181 aactctacaa aattttcgtt taaccacttg gcatcctgga taaacatacg cacacaacca
     6241 tggctagccc ttacccccgg aatatcatca gaaccatgta aagcaaaccc tttatgaaaa
     6301 tacatgcaat aaggcatttt tgcaccgcct ctgcctatag gaaaaacatc tgaagtacaa
     6361 cgaacatttt ccttgctgaa tacacggaaa attcctgtca aagtacgaca gactttattg
     6421 gaatcagagc atttatcact tccagaagaa ataggccccc accatactaa attgccctga
     6481 gcatcatagg cgccccaagc cagcttctct tgatccacaa taatttgttt ttcattttca
     6541 ctctggattt taagtgggaa aggggagaca tcaaaaatat tgatgttggc taaattcttt
     6601 ggtacagcga tttctctgcc tgaccaaagg tagttataag tacgattgac tctttgcaca
     6661 atgtctcttt ggacaggatc agggaataat ttttcccaac tttgtccccc ggttacctta
     6721 atacattcat attgaggata agcacataat gccgttccat aaaaatgccc agcgttaagc
     6781 acagtcacca ttgacatcat gaacgttgtt aatagtattt ttttcatggt gccgccccct
     6841 tatgcctgat ttttataatt ttatgcaaaa tttgtgctaa aaatgaaatg ccactctttt
     6901 tatctttttt cggttatttt taatttaagt ttagacccat acaaaaaaaa attgaaatta
     6961 ttgctatatt taaattagca ttgactgggt tgaacactca tgagtccttt aaaaagtaaa
     7021 attaaaacaa agtctttcaa caaattaaca aaaactcaag acattaccaa cacgattgaa
     7081 agattatttg caaactctca agacagcatc attgttttta atcaacaagg caaaattatt
     7141 gctattaatg aaaaagctgc tgctttattt aaaaaaagcc ctgacaaact aattgacaaa
     7201 ccgatttgga aactatttaa gttaaatagc ttcgccatta agaggcaatt tattcaagca
     7261 aagaggtgtt ttcttttagc caatgaaggg atagcccaac aattcacttg gcttgaaagc
     7321 aaagcacaaa aaccagtact tgcataccat atcctgttca ataaaacaga aatagaagac
     7381 acatcaatca tttttaccaa acttaccgat attctaaaat cgaaaaccat agaatggatt
     7441 ttatggtctt taactaaaat aagtaaccac caggaaatca atgaaattat tgatcaaatt
     7501 ctacaattaa ccagtgatgt ttttgcagcg gattatactt ctgtatcttt cataaacaat
     7561 caacaaattg caaggacaat cagctactat aaatttggaa agaaagaaga gaacattagt
     7621 ttttctttaa tcaatacacc ctgcgctaaa gtaatccaga aaaaagctat ctgctatttt
     7681 aatgacgttc aaaagcaatt tccaaacgat aaattattga aaaaaatgaa aatcaatacc
     7741 tatctcgcag gcccaattac aaatccccag gatgaggtga ttggcataat gactatcctg
     7801 tgtaaacgcg aagtcgaatt tgacacactg aataaaacct ggtttggttt atttttaagc
     7861 cgaatcaatt cggagattga gagactgata aaccttagaa aattagagtt tctggcgagt
     7921 ataccccagc aaaaccctaa tccaatcata aggattcttc tcaatgggga tgtcgtattc
     7981 actaacgatg aagggaaaat cattttaaac tactggataa aattatttaa gggacttcca
     8041 gtagagttac taaaagaagc acaaagaatt cagaagtcca atcaaaatac acgaattgaa
     8101 atggaaattg aggataaaat ctacttattc acacttgtct ggattgacga atttaaacag
     8161 atcaatattt acgggactga tataacccag ctcaagaata cgcaaaacgc tatgttaaat
     8221 ctaacccgca gagatacttt aactcaaatc gcaaatcgac aatattttga agaaaaacta
     8281 attgaaaaaa tatatgagca tcatctgaaa gaaaaaagcc tggcattatt attaatcgat
     8341 ttagataact ttaaaattgt taatgataca ttagggcatc caacaggaga caaactactg
     8401 atggctgtta ccaatagaat gacccgatgc ctaagagcaa atgattttat tgcccgcctt
     8461 ggaggagatg agttcatcgt ccttcttgac caatgcaata ccgataccgc cgttaaggtt
     8521 gcggaaaaga ttattaacgt actcgacaaa cctttccaat ttgatgacta tcatatgaca
     8581 atcacagcca gtattggcat tgccatctat cctgaaacaa gtgaaatagc cagggactta
     8641 ctaaagtatg ccgatattgc aatgtaccaa tccaaaaaaa caggtaaaaa caagtgcact
     8701 gtgttttcaa aaaacttaca ctataaggaa aatcaaagaa atgaaattct caaaaaagac
     8761 atagaattag cagcattaaa aagagagctc tatatagact accaacccca aattgactta
     8821 tccgataaca caatagttgg aattgaagct ttattacgat ggtcacaccc tactctgggt
     8881 ttaatattac ctactgagtt tattcctata gcagagcaaa cagggtgtat acatatcatc
     8941 agtcaatggt tcatagaaca gacattactt gattactccc agtttcagca attaaattca
     9001 aacatcaaat tgtcgataaa tgtttccttg agtcaattaa atgacatgcg atttactaaa
     9061 atattgtgtg atagtttact gcaaaataat ttaagcaaga gcaagatcat tttagatata
     9121 tcagaacgaa tgattgcccc acactttatt caaatatcaa aaaaattaaa a
//
LOCUS       DAXLDT010000118         9157 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXLDT010000118 DAXLDT010000000
VERSION     DAXLDT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.118"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(195..1019)
                     /gene="kdsA"
                     /locus_tag="ACF2SP_002271"
     CDS             complement(195..1019)
                     /gene="kdsA"
                     /locus_tag="ACF2SP_002271"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFD2360046.1"
                     /translation="MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLN
                     IPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSS
                     VVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
                     CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALA
                     RAAVAVGISGLFMETHPDPDKALSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF"
     gene            complement(1016..2653)
                     /locus_tag="ACF2SP_002272"
     CDS             complement(1016..2653)
                     /locus_tag="ACF2SP_002272"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFD2360047.1"
                     /translation="MTKYIFITGGVVSSLGKGIAAASLAAILEARGLRVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTNDGAETDLDLGHYERFVKTTMTKRNNFTSGKIYENVIKKE
                     RRGDYLGGTVQVIPHITNEIKRCIKLGADAFDVAMVEIGGTVGDIESLPFLEAIRQMR
                     IELGSQRAIFIHLTLVPYIATSGETKTKPTQHSVKELRSIGIQPDVLICRSEKPLSMA
                     DRAKIALFTNVEKEAVISLEDANSIYQIPMILHAQHLDEIVVKKLSLEAKQADLSEWQ
                     RVVDMQAVQTMTVKIAMVGKYTELNDAYKSINEALLHAGIHTETKVEIIYFDAEMIEK
                     HGALLLESIDAILVPGGFGERGVEGKIKAIQYAREHKVPFLGICLGMQTAVIEFARNV
                     VGLTGANSTEFNKETLYPVLGLISEWMDADGSKQIRDENTDLGGTMRLGGQYCHLAEG
                     TLARKVYGKPQIIERHRHRYEVNNKYVDSLVKHGLIISGRSADNSLVEMIELADHPWF
                     LACQFHPEFTSNPRDSHPLFKEFVLAARIHHQEKDKK"
     gene            complement(2779..3246)
                     /gene="ribH"
                     /locus_tag="ACF2SP_002273"
     CDS             complement(2779..3246)
                     /gene="ribH"
                     /locus_tag="ACF2SP_002273"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFD2360048.1"
                     /translation="MREIKTIVSDGVTFPIALVVSTFNELITSALKKGALDRLTELGF
                     KSEDITLVEVPGAVEIPFVAQLLAKKQKVEVIVALGAVIRGETSHYDYVCDQVSQGCQ
                     RVMLDYNIPVIFGVLTTENDEQALARVGGTHGHKGRDAIDCAVSMRSIKQQLQ"
     gene            complement(3254..4462)
                     /locus_tag="ACF2SP_002274"
     CDS             complement(3254..4462)
                     /locus_tag="ACF2SP_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFD2360049.1"
                     /translation="MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATP
                     EAINFMSRLGCGLICLPMAEELIDKIQLPMMARHNKSPYGTAFTVSIEAANGVSTGIS
                     ARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTP
                     AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPL
                     EENGVFTMTVFENQIDSGEHFVLVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQ
                     LELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTVDANLELGLPADDRD
                     YAVAYQILKYLGVDALRLLTNNPLKIASLERYGMKITQRIPLEIKPSRENHCYLKTKK
                     IKLGHLLAID"
     gene            complement(4459..5073)
                     /locus_tag="ACF2SP_002275"
     CDS             complement(4459..5073)
                     /locus_tag="ACF2SP_002275"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFD2360050.1"
                     /translation="MFTGLIENTGKVLVNRQGENSSRLVVSSSYQDLKPGESIAVNGV
                     CLTLLPADFPELCFDISPETLHVTTVSKLSSGDMVNLERAMLASTRFGGHYVSGHVDT
                     RAKVKSIRAVNEFIAMDLEGFIPEHNHYLIPKGSITVDGVSLTINTVSEQTVGLMLVP
                     HTLSMTNLGSLRVGQQVNIEFDYLTRIVAHQLQIIGKLNQEEVP"
     gene            complement(5058..6131)
                     /gene="ribD"
                     /locus_tag="ACF2SP_002276"
     CDS             complement(5058..6131)
                     /gene="ribD"
                     /locus_tag="ACF2SP_002276"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFD2360051.1"
                     /translation="MHEQFLLAALEQAKLGRGFCAPNPSVGAVAVQNGNIIAQAWHRG
                     AGTPHAEQLLLSQIPPQTPGVTLYVTLEPCNHWGKTPPCVDAIINHGIQEVVFSYFDP
                     NPIVAKSNSSEKLREHGIQVRHIPIKAIDAFYKSYAYWTMTHKPRVTVKIAQTFDGKI
                     GKSEGDRVVLSNVLCEQFTHQMRAASDVILTSAKTVQMDNPRMNARLDSGVVAKPVAI
                     VDRNLSLSDDTIIFSTAKHCHIYHQPQANQAKTYPNSTHYAMPLQNGLMDLDAIITHL
                     GQLGYHDVWVEAGGAIFSALHSKGLVHRTYLYLVPGSLGHQAISAYQQQGIFEKAHTV
                     SWQAMGDNMIACLDWQEDECLPD"
     gene            6194..7630
                     /locus_tag="ACF2SP_002277"
     CDS             6194..7630
                     /locus_tag="ACF2SP_002277"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HFD2360052.1"
                     /translation="MIKLNLNYKLITKRTLPKLFYWGLGIVFFFLAPTTNAGSLSPYT
                     TKELEQLEREFIQLINQSDSVERNPLASQYINSIGKKLAHYGRIKTPYFFIVKSKEIN
                     AFAGPGGYIGINTQLILTSSNESELAAVMAHEIAHVRLHHLYSIIEHQKQMRVPLLAS
                     ILASAALGVLNPTVGMGAMMASVSGFSQDNINFTRSKEKEADRIGIDMLIKAGFNPQG
                     MAAFFNKMQENSRYYYTANIPAILRTHPLDADRIAEAENRTSRLPKKHYTDSLDYALF
                     KELIRVSVGSNNKQILDYYEHHCHKKNPATACQYGYVLALLNNNNFQKAAEKLNPLLL
                     QDNSNLNFQIAMAQAELGLSQYQSALSRLAELQNNFPDNYAALMAYAQGLLTANQPQK
                     ATAVLLKGSRQYKEDLPLCRELARAQAESHQKSYAYFTQAQCLLLEGQKRGAIAQLKV
                     AQKLARKDAYLSARISAKIDEIKFMVEN"
     gene            7909..8844
                     /locus_tag="ACF2SP_002278"
     CDS             7909..8844
                     /locus_tag="ACF2SP_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2360053.1"
                     /translation="MNEIQEFLRQKLNSPLPISRPQLAKLPMGTMEFVTYGNGKPVLI
                     IHGGGGGYDQAVLLFKRYIPDGYQMICPSRPGYLGTPIDTGQTAEEQADALAGLLDYL
                     NITSAVIVAISAGGLSLYPFAIRHPKKTKAIIAIDAIAGEYLMPEQSNKLAQALFMTD
                     FGLWVAKKSMIYFPKSFIKNLIKLEGYLDPVRVDDRVKEILNSRERLEIIAELMNSMA
                     DYKARKTGTLNDMAEGAKTSWYDFSKINCPALIIHGTHDADVKFYNGVFAYERLASKE
                     KERFWIEYGTHFGFFFAHQARKAQQKFREFIIKHG"
BASE COUNT         2822 a         1980 c         1624 g         2731 t
ORIGIN      
        1 tccaatattt tctcaatgca catattgtaa cattttattc atcaagaaaa cattgggatc
       61 aatatgatta tattaatacc attattatct cttttgaatc aaataaaaac ggtatgatta
      121 atcagataaa agggaggagc aaaataaaaa atattcccgt gccatacttc tttttacacg
      181 ggatatcatt taatttagaa atcagtactg tactttttat acacttcatc cgctgctttc
      241 aatgactcca aaagttgttt cattttatct agaggccaac tattgggacc atcacttagt
      301 gctttatccg gatcagggtg agtttccata aataaccctg atattccaac agctacagct
      361 gctcttgcaa gagccggaat aaattctctc tgccccccgg atacaccatt attgcccccg
      421 ggcaattgaa ccgaatgtgt tgcatcataa accactggac agccggtttc acgcatgata
      481 acgagggaac gcatatcaga aaccaaatta ttgtagccga aactcactcc tcgctcacaa
      541 gccataattt gctcattgcc ttgcgccttt gccttcgcaa ttacatgttt catttcccag
      601 ggagcaagaa actgtccttt tttaatatta accggcttat tcatggcagc aactttttgg
      661 ataaaattgg tttgcctgca taggaaggct ggcgtttgta atacatctac cacactggag
      721 acttcaaata agggcgtgtc ttcatgaacg tcagtcaaaa caggaacacc tatttgtgat
      781 ttgacctttt ccagaatcga taaccctttt tcaaagccag gccctctgta actggaaata
      841 gatgagcggt ttgccttatc aaaagaagac ttatagatga aagggatatt caattgactg
      901 cacatttcct ttaaatatcc tgcggtctct aaagccagtt cttcactttc aataacacaa
      961 gggccggcaa tcaaaaacaa tggcttatct aagcctgctt caaaaccaca taatctcatt
     1021 ttttgtcctt ctcttgatgg tgaatacgag ctgcaagtac aaattcttta aatagaggat
     1081 gactatctct tggatttgat gtgaactcag gatgaaactg acaagctaaa aaccaggggt
     1141 ggtctgctaa ttctatcatc tcaaccagtg aattatctgc tgatcgtcct gaaataatta
     1201 agccatgttt tactaaacta tccacatact tgttattgac ttcatatcga tgtcgatggc
     1261 gctctataat ttgtggtttg ccataaactt ttcttgccag agtgccttca gcaagatgac
     1321 agtattgtcc gcccaagcgc atggttccac ccaagtcagt attttcgtcc ctgatctgct
     1381 tgctaccatc ggcatccatc cattcactaa ttaaaccaag cactggatac aatgtttctt
     1441 tattgaactc agtcgaatta gctcctgtta aaccaactac atttctcgca aactcaataa
     1501 cagctgtttg catacccaaa catatcccta aaaaaggaac cttatgctcc cgagcatatt
     1561 ggatagcctt aattttgcct tcgactcctc tctcaccaaa accaccaggt accaaaatag
     1621 catcaatgga ttcaagcaat aaagcgccat gcttttctat catctcagca tcaaaataga
     1681 taatttctac ttttgtttcc gtatgaattc cagcatgcaa taaagcttca ttaatcgact
     1741 tgtaggcatc attcagctcc gtatatttac ctaccatagc gattttcact gtcatcgttt
     1801 gaactgcttg catatcaact actcgttgcc actcgctcaa atcagcctgc ttagcttcca
     1861 ggcttagttt cttaacaaca atttcgtcta aatgctgcgc atgtagaatc ataggaattt
     1921 gataaatact attcgcatcc tcaagagaaa tcacagcttc tttttcaaca ttagtgaata
     1981 atgcgatttt agccctgtct gccatggata atggtttttc tgaacggcaa atcaatacat
     2041 ctggctggat accaatagaa cgcaattcct ttaccgaatg ttgagttggt ttggttttgg
     2101 tttccccaga agtagcaata taaggaacca aagtaagatg aataaatatt gctcgttgtg
     2161 aacctaactc aatacgcatc tgcctaattg cttcaagaaa gggtagtgat tcaatatcac
     2221 caacagttcc tcctatttca accatagcca catcgaatgc atccgcaccg agtttgatac
     2281 agcgtttgat ttcattagta atatgaggaa taacctgaac tgtccctcct aaataatcac
     2341 cccgtctttc tttcttaatt acattttcgt aaatttttcc cgatgtgaaa ttattacgct
     2401 tggtcattgt tgttttaaca aagcgctcat aatgccccaa atccaaatcg gtctctgccc
     2461 cgtcattcgt tacgaaaacc tcgccatgct gaaagggact catagtacca ggatcaacgt
     2521 taatgtaggg atcaagcttt atcaacgtaa cgcgaagacc acgagcttca agaatagctg
     2581 caagagatgc ggctgctatg ccttttccta aagaagacac aaccccgcca gtaataaaaa
     2641 tatattttgt cattttttga ccccgctcaa aatgccgaat ccaccaattt ggtgttcaat
     2701 ttaatttgca tgtaaacggg attcaattct atcaaaatga aaatataaat aacagaaatt
     2761 tgagcaaaat taatagaact attgtaattg ttgtttaata gaacgcatgg aaaccgcaca
     2821 atctatcgca tctctccctt tatggccgtg agttcctcct actcttgcca aagcctgctc
     2881 atcattttca gtagtcaata caccaaagat aaccgggata ttataatcta acataaccct
     2941 ttggcatccc tggctgacct gatcacaaac gtaatcataa tgagaggttt caccacggat
     3001 aactgctcca agtgccacta tgacttctac tttttgtttt ttcgctagca actgtgcaac
     3061 aaacggaatc tctactgcac caggaacctc aactaaagta atgtcctccg atttaaaacc
     3121 cagttcagtc aatctatcta acgccccttt ttttaatgca gaggttatca attcattaaa
     3181 agtacttaca actagcgcta ttggaaacgt gacaccatca ctaacaattg tttttatctc
     3241 tctcatgttt atcctaatca atggctaata aatgccccag ctttattttt ttggttttta
     3301 aataacagtg attttctctg gagggcttaa tttccaaagg aatacgttga gtaattttca
     3361 ttccgtaacg ctcaagagaa gctattttta aaggattatt tgtcagcaac cttaatgcgt
     3421 caacccccaa atatttcagt atttggtatg ctacggcata atctctatcg tcagcgggca
     3481 atcctaattc cagattagca tcgactgtat caaacccctg ctcttgcaaa gcatacgcct
     3541 ttaatttgtt tgccaaacct attccacggc cttcctgacg caaataaatt aatataccac
     3601 cttcagctcc gatctgggat aaagacaact ccagttgctt tccacaatca cattttctgg
     3661 agccaaaaac atcacctgtt atgcactcag aatgaattcg aaccaaaggc acttgattac
     3721 catacaaagg gggtttaacc aagacaaaat gttcaccgga atctatctga ttctcaaaaa
     3781 cagtcatagt aaaaacacca ttctcctcca gaggaatctg agtggttgcg acaggattaa
     3841 taagattttc gtgacgaatt cggtaattaa tcaaatcttt aatggtgact aaaggtattt
     3901 gatgtttttt tgaaaaaaga gctaattcat cacgtctgct cattgttcca tcttcattaa
     3961 tgatctcaca aatcactgcc gccggggtca atccggcaag tcttaccaaa tcaacactac
     4021 cttctgtttg acctggtctt tctaaaactc ctctctttct agcccgtaaa ggaaaaacat
     4081 gtccaggtga aatgatatca gatggcccac tttttggatc aatagccact tgaattgttc
     4141 gagctctgtc tcttgctgag atgcctgtgg agacaccgtt agctgcttca atcgaaaccg
     4201 taaacgccgt accatacggc gatttgttat gccgagccat catgggcagc tggattttgt
     4261 ctatcaattc ttcagccata ggcaaacaaa ttaaaccaca accaagacga gacataaaat
     4321 taatggcttc aggagtagcg tgctcagcag caatcaccaa atcaccttca ttctctctat
     4381 cttcatcatc cattagaata atcattttac cggctttaag tgtcgccaca gcctcttcta
     4441 ttgttgctaa tgaatgtttc atggcacttc ctcttggttc aatttcccga taatttgaag
     4501 ctgatgagcc acaatacggg taagataatc aaattcaata ttgacttgct ggcccactct
     4561 taatgaaccg agattggtca ttgataatgt atgtggcacc agcatgagtc cgacggtttg
     4621 ttctgataca gtattaatag tcaagctaac tccatctact gtaatgctgc ctttgggtat
     4681 taaataatga ttgtgttctg ggataaaacc ctccaaatcc atcgcgataa attcattaac
     4741 agcacgaatg gatttcactt tagctctggt gtctacatga cccgatacat aatgaccacc
     4801 aaagcgggtt gatgccagca tagctctttc aagattaacc atgtcgccag atgataattt
     4861 tgacactgtg gtaacatgca gagtttcagg tgaaatatca aaacacagtt caggaaaatc
     4921 tgctggcaaa agggttaaac aaaccccatt tacagcgata ctctcaccag gtttcaaatc
     4981 ctgatagctt gaagaaacga ctaaacgact agaattttct ccttgcctat tcactaatac
     5041 tttgcctgta ttttctatca atccggtaaa cactcatcct cctgccaatc caaacatgct
     5101 atcatattgt cacccatagc ttgccaactc accgtatgag ctttttcaaa aatcccctgt
     5161 tgttgataag ctgaaatcgc ttgatgccct aaactgcccg gaactaaata caaataggtt
     5221 ctatgcacca atcccttgct atgtaacgca gaaaaaatag ctccgcccgc ttcaacccat
     5281 acgtcatgat atcccagttg accaagatga gttattattg catccaagtc catcaaccca
     5341 ttttgcaaag gcatcgcata atgagtgcta ttaggataag tcttggcctg atttgcttgt
     5401 ggttgatgat aaatgtggca atgttttgct gtcgaaaaaa tgattgtgtc atcagacaaa
     5461 cttaaattgc ggtcaacaat ggcaactggc tttgctacca ctccactatc taatctggcg
     5521 ttcattcttg gattatccat ttgaactgtc ttggcactgg ttaatatgac gtcactggca
     5581 gcgcgcattt gatgagtgaa ctgttcgcat aaaacattgg acaaaaccac tcgatctccc
     5641 tcagactttc caattttacc atcaaaagtt tgtgctattt taacagtgac tcttggttta
     5701 tgggtcatag tccaataagc atagctttta tagaacgcat caattgcctt tataggaata
     5761 tgcctgactt gtattccatg ttcacgtaat ttttctgagg agttactttt ggcaacaatg
     5821 gggtttgggt caaaatagga aaaaacaacc tcctgtatac catgattgat aatagcatca
     5881 acacaagggg gagttttacc ccaatgatta caaggctcca gtgtgacata aagcgtaacc
     5941 cctggcgttt ggggtggaat ttgtgataac aataattgtt ctgcatgcgg tgtacctgca
     6001 cctctatgcc atgcctgtgc tataatatta ccattttgca cagcaaccgc cccaacgctg
     6061 ggattggggg cacaaaagcc tctccccagt ttggcttgtt caagtgctgc aagcagaaac
     6121 tgttcgtgca tatattaacc accttcttaa tgtggtcgaa tgataacaaa ttattcaatt
     6181 attgagtagc ctaatgatta aattgaactt aaattataag ttaatcacga aaaggacatt
     6241 acctaaacta ttttactggg gtttggggat cgtcttcttt tttttagcac ctacaactaa
     6301 tgccgggagt ctttcaccct atacaaccaa agaactggaa caattagaaa gagaatttat
     6361 tcaacttatt aatcaatcgg acagcgtaga gcgcaatcct ttggccagtc aatacatcaa
     6421 ctccatagga aaaaaactgg cacattatgg gcgtatcaaa acaccttatt tttttattgt
     6481 taaatccaaa gaaatcaatg catttgcagg tcctggaggg tacataggca ttaataccca
     6541 actcatttta acgagcagca atgaaagtga actggcagct gtaatggctc atgaaattgc
     6601 gcatgtacgc ttacatcatc tttacagcat tatagaacac caaaaacaga tgcgtgttcc
     6661 attattagcc tctattctgg cgtctgcagc cttgggagtc ttgaatccaa ctgtcgggat
     6721 gggcgctatg atggcatccg tttccggatt ttcccaggac aacatcaatt ttacacgctc
     6781 aaaagaaaaa gaagctgaca gaattggtat agacatgctt attaaggcag gcttcaatcc
     6841 tcaaggtatg gccgcatttt ttaataaaat gcaagaaaat tcccgctatt attacactgc
     6901 caatattcca gcaatactgc ggactcaccc cctggatgct gacaggatag ctgaggctga
     6961 aaacagaacc agccgtttgc ctaaaaaaca ttatactgat tctttggact atgccttatt
     7021 taaagagtta atcagagttt ctgtcggctc taataataaa caaattcttg attattatga
     7081 gcatcattgc cataaaaaaa atcccgccac tgcttgccag tatgggtacg tacttgcctt
     7141 gttaaataat aacaatttcc aaaaagcggc tgaaaaatta aaccctttac tgttacagga
     7201 taatagtaat ctcaattttc aaattgctat ggctcaagct gaattaggtt tatcacaata
     7261 tcaatcagca ctctcccgtc ttgctgaatt acaaaataat tttcctgaca attatgctgc
     7321 tctaatggca tacgcccaag gattgttaac tgcaaaccaa ccccaaaaag ctacagccgt
     7381 acttcttaag ggtagtaggc aatacaaaga ggacttaccg ctttgccgag aattggcaag
     7441 agctcaagct gaatcccatc agaaaagtta tgcctatttt actcaagcac aatgcttact
     7501 acttgagggg caaaaaagag gtgctattgc tcaattaaaa gtggcgcaga aactggccag
     7561 aaaggatgct tatttatccg caagaatttc agcaaaaatt gatgaaataa aatttatggt
     7621 tgaaaactaa tccaaatata gattaattaa aataaaatga tctcatttat cattcgctaa
     7681 gagtgtaagg gtttaatttt gctttgttca ggcccaatat aattaattaa tgcttgcaag
     7741 catctcactt ctgataggtc ttaaatggaa acaaaataga acatatttat caaaaagaaa
     7801 ttagatggca aaaaggtctc ttcatcaata attttcgata ttttcagtag acaagttcat
     7861 ctaaaaagcc aagtattaat ttgctttcac tttggatagg gagttgccat gaatgaaatt
     7921 caagaattct tgcgtcaaaa actgaattcg ccgttaccta tttcacgacc tcaattggca
     7981 aaactaccaa tgggtactat ggagtttgtt acttatggta atggaaaacc cgtactgatt
     8041 attcatggtg gcgggggtgg ttatgatcag gcagtgcttt tattcaaacg ctatatcccc
     8101 gatggttacc aaatgatttg tccttctcgt cctggttatt tgggaacacc cattgatact
     8161 ggacaaacag cagaagaaca ggcagatgcc ctggccggct tactggatta tttgaatatt
     8221 accagcgcag ttattgtagc catatcagct ggcgggcttt ctctgtatcc ttttgccatt
     8281 cgtcacccga aaaaaactaa agcaatcatt gcaatagatg ctatagctgg ggaatattta
     8341 atgcctgaac aatccaacaa attggctcaa gcccttttta tgactgattt tggtttatgg
     8401 gtcgctaaaa aaagcatgat ttatttcccc aagtcattta tcaagaattt aattaaactg
     8461 gaaggttacc tggatccagt acgtgtagat gaccgggtga aagagatctt aaattctcgg
     8521 gagcgcctgg aaatcattgc tgaactaatg aatagcatgg cggactataa ggctcgtaaa
     8581 acagggaccc tgaatgacat ggcagagggt gcaaaaacca gctggtatga tttttctaaa
     8641 attaactgcc ctgcactaat aattcatggg acacatgatg ctgatgtaaa attttacaat
     8701 ggtgtatttg cttatgagcg attagcatcg aaagaaaaag agcgtttctg gattgaatac
     8761 ggcactcact ttgggttttt ctttgctcat caggcgcgta aggcgcaaca aaaatttcgg
     8821 gaatttataa taaagcatgg ttaaacagtt tcttgttgaa aagttcatgg cggcgggata
     8881 aacaacttct aaatctatct actaaataac gtgagttata gataaagagg atataaaaac
     8941 cagtcgaacc ccaggttacg tttgggctga ggagatgcat aagcatcgtc tcgaagcctt
     9001 gacaaaaaaa cattaaatcc aatgctaggc ttcgagacgg cataaatgcc tcctcagcca
     9061 gagcgattcg agataataaa tgattttatc aataactcaa gttaaataca ttgttttagt
     9121 gctataacaa caacgattgg aacaaataag acttaaa
//
LOCUS       DAXLDT010000119         8916 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXLDT010000119 DAXLDT010000000
VERSION     DAXLDT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.119"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            408..1781
                     /locus_tag="ACF2SP_002279"
     CDS             408..1781
                     /locus_tag="ACF2SP_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HFD2360054.1"
                     /translation="MFQSILIIALMTFSFITQAALPADPIQRCLDIRRLVDITSRQKM
                     AAKEPGVLRFKFHKISASELPLDNPVGNMDEVIQALNNQIENCNNSYGEFQTVTIAGH
                     QFKRQEWCLNTNKKMLTLAKAAHGDFKKFLSSIKTEFDWYKSDGWPENHAGFKKGEFQ
                     FTAYYAPAAVEARSKRGGAFLYPLYSNPGVVSVALESKKYNLKTPLCGVDPLSKVMRG
                     FCLKNADGTYSVAPDREEIEHGALNPKYIIGYLKDPNDSAFLMLQGSGSLLLDGKLFH
                     INYDGANGRPRTMLGRIVQCAQDPTCGGNLDTMERCAKDPKCHDEAKLRCNVSKKIRQ
                     SAASEKLIRQYLNNLTPDKADNLRNRDQSYVFFAKEDGGPYGSENISLTPHVSCATDH
                     RVIPVGMNFIYHCKKATSWCVAQDAGGAIIGAHVDVYKGEGNQAGVEANQLNHTGTLF
                     VALPKRK"
     gene            2157..2447
                     /locus_tag="ACF2SP_002280"
     CDS             2157..2447
                     /locus_tag="ACF2SP_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360055.1"
                     /translation="MSIISNHDITFSSTQKDLIEKQQKNDIKNQHIADAKANKGAETS
                     EKLIVFGAKTTALAACGVALLFPLTAPIIVPIAQVSGAVALVIITRKFIKSS"
     gene            complement(2678..2824)
                     /locus_tag="ACF2SP_002281"
     CDS             complement(2678..2824)
                     /locus_tag="ACF2SP_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360056.1"
                     /translation="MAKPLNGTMKSLAKDRLDITQNQANQLISEEMALGDLRLAPKNS
                     RRPW"
     gene            complement(3323..4732)
                     /locus_tag="ACF2SP_002282"
     CDS             complement(3323..4732)
                     /locus_tag="ACF2SP_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2546 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360057.1"
                     /translation="MEMKMSITVKSLDFDQCISNRKYKESLQTNDGRKVWDANSLFNA
                     NKEILGKNNNGDPIHVFIGSNRQNLKADLINLNAGAATLFIPVAQELCDVMGATFHPL
                     LVPDLICENAAIGDTFHSALQVIKGLNDLNSLNSESLAELVKSALSGQLNSLHCISDE
                     SKFLMLYSQIQYMAQQYPDEKINFEFYDDKEDILKPLYDIFSKNPDLIPANVTLHIKR
                     YLNGNLMETDFNPILGLGSQQENYQNIVKWIHKQSSSNLRSGNCCQVLEMDNEKIARY
                     CRFGKDETRLKLLDSLENLAKHQVGQKDQKMDDFIKESYEKMGGSKDIDSITLQQPFE
                     EINSAIKVTEAINKVIANYRKEAKCLFSVGMNAKADRIEKALLNVPVEDRGKIFSNDK
                     VSPELIAIRAALASHRYFGKRGNVYYKDEARTVIDENKAATTYNNLRKQFANLRTQSH
                     ADAQVELEHSSEVSRSLKL"
     gene            complement(4907..5242)
                     /locus_tag="ACF2SP_002283"
     CDS             complement(4907..5242)
                     /locus_tag="ACF2SP_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFD2360058.1"
                     /translation="MTISYEEFEKVDLRSGTVVKAESFLRARKPAYKVWVDFGADIGV
                     LQTSAQITVHYTPEALIGRMVIGCVNLGEKNIAGFLSQFLLLGFSDEKGAICLASVEP
                     HVPNGKKMH"
     gene            complement(5252..6166)
                     /locus_tag="ACF2SP_002284"
     CDS             complement(5252..6166)
                     /locus_tag="ACF2SP_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFD2360059.1"
                     /translation="MVIDVKKSNTYFIQGMLFLIFAQIMVGINIVFSKYVLSSIPVLY
                     ILALRFTLAAVILLLLHWLTPAKKLPVYSYFLQLKRRDWFFIFAQALSAGVLFNFLIL
                     WGLQYTDANVAGIITSALPAIIAIMSWLILGEKISGKKAICVGFATLGLVVIACDKLG
                     DINVSHSFLGDALVLTSLLPEAMYYILSKMHVNSLPVFLISSLLNGINAILLLFCLSF
                     STWDSFTIHTLDWLILIILGLSSGLFYVFWYFGCQTVDGVMASLSTAVMPLATVTIAW
                     IFLGEQLTIGQIIGMGMVILSITVYAKR"
     gene            complement(6232..6987)
                     /locus_tag="ACF2SP_002285"
     CDS             complement(6232..6987)
                     /locus_tag="ACF2SP_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360060.1"
                     /translation="MLLTNRIDLRSYHTESCSHTHDFAQLVLPVSGSMELEVGHYSGV
                     VNDDIGVYIAPNEQHCFAGSQKNLFLVIDATTQNSLRDEAFESNILNLTASTKKLIQF
                     THHYLAHNERDFFTDSLINQLLFHFAASSFSPEPDPVVIRAKQWIDLYFADSVDVSRV
                     AKHCYLSISQLQRRFKQMLGRGIAEYWRMKKLLHAKKLLSQKNCSIEAIAFEVGYENL
                     PAFSRRFSKVFGESPSQWRAKALTAKKMREIDK"
     gene            complement(7245..7841)
                     /locus_tag="ACF2SP_002286"
     CDS             complement(7245..7841)
                     /locus_tag="ACF2SP_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360061.1"
                     /translation="MGSTLGKWIGLIAAFLFLNNGFAQASARPIICDQEYALCTSARC
                     IPTPGSVAKAICDCVVEKGNSAGYKTCEERKPVRGRYKVTSLISTFSFEQFTTKRPMN
                     CPEGLPWSNCVDMPCTVDPQNSKRALCICTIESTQAFFTFGGDCNTNTCATGFWSGAT
                     QENSIILRNALMQEMRSKPKELPRACPAKSSQANQGQS"
     gene            8002..8142
                     /locus_tag="ACF2SP_002287"
     CDS             8002..8142
                     /locus_tag="ACF2SP_002287"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HFD2360062.1"
                     /translation="MCSYQHHERIDGSGYPNGRKGDEITMVARMVAIADVYEAMSTNR
                     SY"
     gene            complement(8272..>8916)
                     /locus_tag="ACF2SP_002288"
     CDS             complement(8272..>8916)
                     /locus_tag="ACF2SP_002288"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HFD2360063.1"
                     /translation="HCRPNINPTLPQFIIGYGSLMQEESKKEDSSMVGENFPIYVSGF
                     QRGWIERGTPIGFSTIYLGVVPKKDSIINAVYFKLNDPSQIKNYDKRENTYCRIKVPR
                     DDIQALSSINLPEGQFWIYTTSGKQLAAPSSDYPIVQSYVDIFLSGCFSLEEKYHLKN
                     FARDCIKTTSNWSGHWVNDRIYPRTAFDNIPYVAKIDPLIAVELPQFFKLIKIE"
BASE COUNT         2870 a         1729 c         1591 g         2726 t
ORIGIN      
        1 tgcccaataa aaaattatgg atttattgtt atgttgaaca ctatgattaa ttaatagcga
       61 ggttcaatca aacactgagg ctaaccgatg ctatacgcca tatttgacaa taacttaaaa
      121 agtcttttct ggacaataaa gtttcaagct taagaagaag tgcttcaatc gcaggtaagt
      181 taagagcatt aactgatgta tgtaagcatt tggtttgaaa cgctttttaa acagggacaa
      241 ttgggattcg acactatttc tgtataatga tagggtagtt atagggtggc tgaatttatg
      301 taaattcatc tctttgatga gttggccaat agcctatata attcatgtta ctaattgaaa
      361 tcaatggacg ggcaatgcct caaccatagc taagctaaag gataccaatg tttcaatcca
      421 ttctaataat tgcactaatg acttttagtt tcatcactca agcagccctc cctgctgatc
      481 caatacagcg ctgtctggat attagaaggc tcgtggatat cacctcaaga cagaaaatgg
      541 cggcaaaaga acccggggtg ttacgtttta agtttcataa aatttcagca tcagaactcc
      601 cgttagataa ccctgtgggt aatatggatg aagttatcca ggcattaaat aatcagattg
      661 aaaactgcaa taatagctat ggcgaattcc agacagtaac aatcgctggc catcaattca
      721 aacgccagga atggtgctta aataccaaca aaaaaatgct gactctggca aaagccgctc
      781 atggtgattt taaaaaattt ttatcgagca tcaaaaccga gtttgactgg tataaaagcg
      841 atggctggcc ggaaaaccac gcaggattta aaaaaggtga gtttcaattt actgcctatt
      901 acgctccagc cgctgttgaa gcccgatcta aacgtggtgg agcatttctg tatcctctct
      961 atagtaaccc cggggttgtt agcgttgcgt tagaaagtaa aaaatataat ttgaaaactc
     1021 ctctttgcgg agtggatccg cttagcaaag tgatgcgtgg attttgtctg aaaaatgccg
     1081 atggcaccta ctctgtagca ccagatcgag aagaaattga gcatggggct ttaaatccaa
     1141 aatatataat tggctatctt aaagacccaa atgactctgc ttttttaatg ctacaaggct
     1201 ctggttcatt actccttgat ggcaaattgt ttcacatcaa ttacgacggt gccaacggca
     1261 ggccacgcac gatgcttggt cgcatagtcc aatgcgctca agatccaacc tgtggtggca
     1321 atcttgacac aatggaacgt tgcgcaaaag atccgaaatg ccatgatgaa gctaagttac
     1381 gctgcaacgt atctaaaaaa atcaggcaaa gcgctgcatc agaaaagctc attcggcaat
     1441 atctgaacaa tcttacccct gataaagcag acaatttacg gaatcgagat caaagctatg
     1501 tattttttgc caaagaagat ggtggccctt atggttcaga aaatatttct ctaacccccc
     1561 atgtgtcatg tgcaaccgat cacagggtta ttcctgttgg aatgaatttt atctatcatt
     1621 gcaaaaaagc tacttcatgg tgtgtggctc aggatgcagg cggcgccatt ataggggctc
     1681 atgttgacgt ctataaaggt gagggaaatc aagcaggtgt ggaagcaaat cagttaaatc
     1741 atactggaac gttatttgtg gcactaccta aacggaaata gaaagaaggc aatcaatact
     1801 tatttcgagt aattactttt attggctata gtcatttagg gtgctgttaa tctacaaccg
     1861 aataaataag aacgcttttt gatttggatt actttgcgca acgagcaagg aaaatgcggc
     1921 taagcttttt cctatgatct gagaacaaaa tgattccatt ggagtactga ttaacatcct
     1981 ccaagctcgc cattaaaacc acatattttt aatacatatt cagcatttat attatataac
     2041 ttattgaaaa aaaaagatat tacaaatagt atatatcaca tatgattaca attcattgtc
     2101 tattttttaa tctttatgat taaatataat taatcggttc gcttctggag agtattatgt
     2161 ccatcatatc taatcatgac atcactttta gttccaccca aaaggatttg atcgaaaaac
     2221 agcaaaaaaa tgacattaaa aatcaacaca tcgcagatgc aaaagcgaat aagggagcag
     2281 aaacatcaga aaaattgatt gtttttggtg ccaaaacaac agctcttgcg gcatgtggtg
     2341 tcgctctact ctttccgcta acagcaccaa tcattgtgcc catagcacaa gtcagtggtg
     2401 cagtggcact agtcatcatt actcgtaagt ttatcaaaag ctcctaacag cgcctttagg
     2461 cgctagaatg caatgggaac gcttgtatta ctttccattg caatgagagc taatttcaga
     2521 aactccatgt atttctattg gagcagctct atcaggaaat tcagccatta attttaatga
     2581 tccttccata actgaactat atttactcaa agttgaaatt aacctatcag aacatttttc
     2641 caaacgagag attccatctt atttcatatg taatatttca ccaaggtctt cttgagtttt
     2701 ttggtgccag acgtaaatca cccaaagcca tttcttcaga aatgagttga ttggcttggt
     2761 tttgagtaat atccaaccta tcctttgcga gagatttcat tgtgccatta agtggtttcg
     2821 ccatgaaaca acttaatgtt ttaaatttga taaatgttca tcaaatatca aatctttcgt
     2881 taaaattata tcgttataaa gctgcgtttc cttgccatct gattacactc cacaaacgga
     2941 cggagttgcc tttcgcacag ggccaaattt ctctaaaact tttaaatgag ctaaaccttc
     3001 gttttgaacc attccatttt gtatggactc acaacttaaa cctatctcac tcaaataaaa
     3061 aattggcaat cgatacatga atctctcaga tctctgctat caaatattac cttattatta
     3121 caaagaacat tgcttaaaaa tgacagttta atggggtctg tttacatttc gtttcgcata
     3181 gcgaaaatgt ggctaattgg cgccaattag ctgcatttgc agccgtgaaa tgaaatgtaa
     3241 acagacccta agcttttggt agcaataaaa caagccggaa ctggcgaaca aatccggctg
     3301 gaatagaaat ttatagaacc atttataatt ttagagatct tgaaacctca gacgaatgct
     3361 ccagttcaac ctgggcatca gcatggcttt gtgtccttaa attcgcaaat tgctttctta
     3421 aattattgta agtcgttgcc gctttatttt catcaatcac ggtacgtgct tcatctttat
     3481 aatagacatt cccccgcttc ccaaaatagc ggtgtgaagc caaagctgct ctgatcgcaa
     3541 ttaattccgg agagactttg tcattagaga aaattttacc tcgatcttcc actggtacat
     3601 taagtaatgc cttttctatt ctgtctgctt tggcattcat gccaacgcta aataagcatt
     3661 tggcttcctt gcgataattt gcaatgactt tgttaatcgc ttcggttact tttattgcac
     3721 tattaatttc ctcaaatggt tgttgcaacg ttatagaatc tatatctttt gagccaccca
     3781 ttttttcata agactctttg ataaagtcat ccattttttg atctttttgg cccacttgat
     3841 gtttcgcaag attctcaaga gagtctaata gttttagcct tgtctcatct tttccaaatc
     3901 ggcagtatct tgcaatcttt tcattatcca tctctaatac ttggcaacaa ttccctgacc
     3961 ttaggttgct gcttgattgt ttatgaatcc attttacaat attctgataa ttctcttgtt
     4021 gagatcccaa gcccaaaatt ggattaaaat cagtctccat aagattgcca ttcaaataac
     4081 gtttaatatg caaagtcaca ttagcaggta ttaaatcagg atttttagag aagatgtcat
     4141 acagtggctt taatatatct tccttgtcat cataaaactc aaaattaatc ttctcatcag
     4201 gatattgttg agccatatac tgtatctgtg aatacagcat taaaaattta ctctcatcgc
     4261 ttatgcaatg gagtgaattt aattggccac tgagcgccga ttttaccagt tctgctagag
     4321 actcactgtt taatgagttc aagtcattca agcctttaat cacttgcagg gcactgtgaa
     4381 aagtatctcc aattgcggca ttctcacaga ttaaatcagg aacaagtaat ggatgaaaag
     4441 ttgcccccat gacatcgcac aactcttgag ctactggtat aaataaagta gctgctcctg
     4501 catttaaatt gattaaatcc gctttgaggt tttgcctgtt agaaccaata aaaacatgga
     4561 tgggatcgcc attattattt ttacccagaa tttccttatt ggcgttaaat aaactgttag
     4621 catcccaaac tttcctccca tcatttgttt gtaaagactc tttatatttc ctgtttgaga
     4681 tacattgatc aaaatccaag gacttaactg ttatgctcat tttcatctcc aagataacaa
     4741 taattatttc gatatgttta attattttac atattgcact gttattttga aaacgaattt
     4801 tatttatatc tgatattatt atttttaata tcaatgaatg taatcaatgg agctaaaaac
     4861 aaatccatag ttgataatga tgatgtatca atatttcaga taggaatcag tgcatttttt
     4921 taccattagg aacatggggc tcaacggaag ccagacatat agcacccttc tcatcggaaa
     4981 aaccaagcaa tagaaattga gagaggaatc cggcaatatt tttctcccct aaattcacgc
     5041 atccaatgac catccgccct atcaatgctt ccggcgtata atgcactgtt atttgcgcag
     5101 aggtttgtag aacgccaata tcggcaccaa aatccaccca aactttatag gcaggtttcc
     5161 tggcacgcag gaatgattct gctttcacaa cggtaccgga acgcaaatcg actttttcaa
     5221 attcttcata agatatcgtc atttttccac ctcaacgttt tgcatagact gtaattgaaa
     5281 gtataaccat gcccattcca attatttgcc ctatcgttaa ttgctcgcca agaaatatcc
     5341 aggcaatagt cactgttgcc aaaggcatta cagcggtcga taacgaagcc atgactccat
     5401 caactgtctg gcaaccgaaa taccaaaaaa cataaaaaag gccagaactt aatcccagaa
     5461 taattaaaat tagccaatcc aaagtatgaa tagtgaaact gtcccatgtg ctaaagctta
     5521 agcaaaataa caataaaatc gcattaatcc cattaagtaa ggaagaaatt aaaaatacag
     5581 gcaatgaatt aacgtgcatt ttacttaaaa tataatacat ggcttcaggc agaagcgaag
     5641 ttaaaacgag tgcatcacct aaaaaagaat ggcttacgtt gatgtcgcct agcttgtcgc
     5701 atgcaattac aactaatcca agagtagcaa aacccacaca aatggctttt ttgcctgata
     5761 ttttctcgcc aagaattagc cacgacatga tagcaataat cgcgggcaaa gcactcgtta
     5821 taatgccagc tacgttagca tccgtatatt gtaagcccca taatataagg aaattaaaaa
     5881 gtactcccgc agataatgcc tgagcaaaaa taaaaaacca atctcttcgt tttaactgta
     5941 aaaaataaga ataaacaggt aattttttcg ctggggttag ccaatgcaac agtaataaaa
     6001 tgacagcagc cagtgtaaaa cgaagtgcaa gtatgtaaag aacaggaatt gacgaaagta
     6061 cgtattttga aaatacaata ttaatgccta ccataatttg ggcaaagata agaaagagca
     6121 tgccctggat aaaataagta ttggattttt tcacatcaat aaccatcttg gtcttattta
     6181 ataatcgata ccatcaaatg aattcgtagt ctatcaattt aaaaccagag cttatttatc
     6241 tatttcacgc attttctttg ctgttaacgc tttggctctc cattgagaag gggattcacc
     6301 gaatacttta ctaaaacggc gactaaatgc gggcaagttt tcataaccta cttcaaatgc
     6361 gattgcttca atggaacaat ttttctgtga taataatttt ttagcatgga gtaatttttt
     6421 catccgccaa tattcggcaa taccacggcc taacatctgt ttaaaacggc gctgcaattg
     6481 actaatactc aaataacaat gtttcgctac cctgctcaca tccacagaat ctgcaaaata
     6541 aagatcaatc cattgttttg ctctgataac aaccggatcc ggttcgggtg aaaaagaact
     6601 tgccgcaaag tgaaacaata attgattgat taaagagtct gtaaaaaaat ctctttcatt
     6661 atgtgctaaa taatggtggg taaattggat gagcttttta gtactggcgg ttaaattgag
     6721 aatattagac tcaaaagctt cgtcacgaag agagttttga gtagttgcat caatcactaa
     6781 aaacagattt ttttgacttc cggcaaaaca atgttgttca ttaggcgcaa tgtatactcc
     6841 gatatcatca ttaacgacac cggaatagtg acctacctct agctccatag aacccgagac
     6901 aggcagtacc aattgcgcaa aatcgtgagt gtgactgcaa ctttcagtat ggtaagaacg
     6961 aagatcaata cgattagtca gcaacatatt gctcccaagc gagcatttac ctttaaaaga
     7021 aacagattat atcatacttg atttgaaacc attgagagag gtagccagga aactttggat
     7081 tagcttgagc ttttatttca taaaacccaa agggataggt cgggactgac agaggcttaa
     7141 aatgcctggc caaagcttat tgaaaataga cctcgtgatt gctcaggatt tgctcttgtt
     7201 atattttgga cataacagat tctttgaagc attataaatt ttaatcaact ctgcccctgg
     7261 tttgcttgtg atgatttagc agggcaagcc ctgggtagtt ccttaggttt cgaacgcatt
     7321 tcttgcatta atgcattacg aagaatgatg ctattctctt gggttgcacc agaccaaaaa
     7381 ccggtagcac aagtattcgt attacaatca ccgccaaaag taaaaaaagc ttgagtgctc
     7441 tctatagtac agatgcacaa agcacgtttt gaattttgtg gatcaacagt gcacggcata
     7501 tcaacacaat tcgaccaagg taatccttct ggacaattca taggtctttt agtagtaaat
     7561 tgctcaaatg aaaaagttga aataagtgat gtcactttgt atctgcctcg aacaggcttt
     7621 ctctcttcac aagttttata accagcactg ttaccttttt ctactacgca atcacaaatt
     7681 gctttggcca cacttccagg tgtaggaata cagcgagcag aagtacaaag cgcatactcc
     7741 tgatcacata taattggcct tgcagaggcc tgtgcaaaac cattattaag aaataaaaaa
     7801 gcagctatta atccaatcca ttttccaagt gttgagccca ttgttgaggt ccctctgttc
     7861 ttttttttat cacagtatct ttatcatatt atcgaatttt cataattcaa aaaaagcatt
     7921 ggagtttact atactgaata tattattaag ggaccaaatg agtaagataa ttgaacatat
     7981 gaattttcct taggacatta aatctgtagt taccaacatc atgaacgcat cgatggttct
     8041 ggttacccca acggacgcaa aggggatgaa atcaccatgg tagcacgcat ggtggctatt
     8101 gcggatgtgt atgaagccat gtcaacgaat agatcttatt gacatttcat gttgcaaata
     8161 actatcttaa agcatctgga ggaaggtaaa gaggttttgt ctgatgccag ttatgttgag
     8221 tgaatacgtg aaagggggca gatacaacat aaaaagctat cattgcttac ttcactctat
     8281 tttaattaat ttgaagaatt gtggaagttc tactgcaata agcggatcaa tttttgcgac
     8341 ataagggata ttatcaaatg cggttcttgg ataaattctg tcgttgaccc aatgacctga
     8401 ccaattggac gtggttttaa tgcaatccct cgcaaaattc tttaaatgat atttttcttc
     8461 taaactaaag caacctgata aaaaaatatc aacataagat tgcacaatag gataatcact
     8521 ggaaggagca gcaagttgtt tcccactcgt tgtataaatc caaaactgcc cttcaggtag
     8581 attaatggag ctcagggctt gaatgtcatc tcgtggaact ttaattcggc aataagtatt
     8641 ttcacgtttg tcgtaattct taatttgcga tggatcattt aacttaaaat aaaccgcatt
     8701 gatgatactg tcttttttag gtactacacc aagataaatg gtactaaatc caatcggtgt
     8761 gcctcgttca atccaccccc tttgaaaacc tgatacataa attggaaagt tttctcctac
     8821 cattgagctg tcttcttttt tggattcttc ttgcattaac gatccatagc caatgataaa
     8881 ttgtggcaaa gttggattaa tattaggccg gcaatg
//
LOCUS       DAXLDT010000120         8785 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXLDT010000120 DAXLDT010000000
VERSION     DAXLDT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.120"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            51..485
                     /locus_tag="ACF2SP_002289"
     CDS             51..485
                     /locus_tag="ACF2SP_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HFD2360064.1"
                     /translation="MKLWKVLCSFVIAVCYLPLAFAASQSPAGTWTTIDDKTGAKRAV
                     VNLKVSNGTLSGTIVKVYPQPGDTGICSKCPGAFKDKKIQGLTFVWGLKDEGNGVWSG
                     GSILDPKTGKIYKAKMTLQGNKLYVRGYFGVSALGRTQTWIR"
     gene            663..2045
                     /gene="dnaB"
                     /locus_tag="ACF2SP_002290"
     CDS             663..2045
                     /gene="dnaB"
                     /locus_tag="ACF2SP_002290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFD2360065.1"
                     /translation="MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNK
                     LCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANN
                     TPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
                     QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVA
                     GRPSMGKTTLVMNMAEHAAIKAGKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGK
                     LDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKEHGSLGLIVVDYLQL
                     MKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRE
                     SGAIEQDADLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFED
                     LAFNGYQGVE"
     gene            2047..3120
                     /gene="alr"
                     /locus_tag="ACF2SP_002291"
     CDS             2047..3120
                     /gene="alr"
                     /locus_tag="ACF2SP_002291"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFD2360066.1"
                     /translation="MSRPTRLVIEPSALLHNLSQIKHLAPGKKVIAMVKANAYGCGVR
                     EVAPVLDGRIEAFGVACLEEALAIRALGVETPCILFQGVFSSDELSVAVENDFACVLH
                     HAQQLEWLIKTPLPYPIKVWVKVNTGMHRLGFKIHELQKVMGALQTCTWVDKSIGLMT
                     HLACADEPHRPENQQQISLFQEISIPGFRQRSIANSAAIISFPDSQADVVRPGIMLYG
                     VSPFANQNAHDLGLIPVMRFMSAISAIHDNPSFAQVGYGGTWKSDKPSRIGVVAAGYG
                     DGYPRHISEKTPVWVRGREVSIVGRVSMDMLTIDLTEHPDIEIGDEVELWGTHVLVER
                     IAKSAGTVGYELLCQISERVRYK"
     gene            3193..3642
                     /locus_tag="ACF2SP_002292"
     CDS             3193..3642
                     /locus_tag="ACF2SP_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HFD2360067.1"
                     /translation="MKKLIVASLSLTFMLGGCASMNKEGVGTITGGVVGGLLGSQFGG
                     GSGKVAAAGAGALLGAYLGGQIGKTMDKVDRLEMQRALETAPTGKAVAWSNPDTGYRY
                     TVQPTRTYYNAQQPCREYITKAMIGGKTQQIYGKACRQADGSWRVVN"
     gene            3950..4384
                     /locus_tag="ACF2SP_002293"
     CDS             3950..4384
                     /locus_tag="ACF2SP_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFD2360068.1"
                     /translation="MAHLIHSVLPVPPRKIAYIHPDDSVKECIKQMVEKDIGALVVFD
                     NDAHLIGIVSERDILRCYFHKSLSLETAKVSDVVYTNVTILSPHDSVEKAMQVITETK
                     RRHVLIQEEGELLAILSIGDLLYHVLEDKLRVIEHLENYIHS"
     gene            4493..5680
                     /locus_tag="ACF2SP_002294"
     CDS             4493..5680
                     /locus_tag="ACF2SP_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360069.1"
                     /translation="MKTMKQMIRLTMLLLMLSFNLWAINSSSPWYTEEKGKGLVINVE
                     LYLSSTCPHCHKADEFFKEIEKHYTWLHVERYIIDKDKKALKRFGDLLTELNRYDFAV
                     PSIFFCNSRWVGFATAQTTGKDLLNGIEYCKEEIVKNGRLTPATENVLNRWGNASLFD
                     SNITGSPSTNTFIFVVAIIDAISPCALFTITAFLGLLFMIEKRKLQFISGSVFILTVA
                     GVHYFQQVYPTLFFESLSWFRIPAALVGLFAFYFAGQYYKKHSIQPLFIVLAFLLALT
                     VPMFQQTCLMNWSYVFEQWLHNQNVSGVQMGLYQFAYQLIYIFPLIILMFLYWILMKI
                     HFFKKFKTKLTTIGFLYILAIALLLIVYPYALSNLALSLFLIVSLGISGLLLNWFNAS
                     KMT"
     gene            5792..6088
                     /locus_tag="ACF2SP_002295"
     CDS             5792..6088
                     /locus_tag="ACF2SP_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HFD2360070.1"
                     /translation="MDDNNSEFKHLFPIAVEVTEGKTYVWCGCGKSKTQPFCDKENCG
                     DKAVSFIAELTEDVYFCNCKQTKNPPFCDGSHAKILLEVVKKKAKQMNRKGSLK"
     gene            6085..7635
                     /locus_tag="ACF2SP_002296"
     CDS             6085..7635
                     /locus_tag="ACF2SP_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFD2360071.1"
                     /translation="MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIG
                     INYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGV
                     ESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQIT
                     KVDTPQKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTII
                     DNLTELVVESFNVEQNRFKGFLQQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQ
                     DNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIREEEYAKQVSELQAR
                     LQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYDEHILDSFQRWKRSSSALSLAV
                     CDIDHFKRINDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKE
                     AFGILEKLRKLVEDCLFYYRDKKVDVTVSFGLTTVTEEDDIESLFMRADNAMYKAKNA
                     GKNRVEIL"
     gene            7787..8776
                     /gene="lipA"
                     /locus_tag="ACF2SP_002297"
     CDS             7787..8776
                     /gene="lipA"
                     /locus_tag="ACF2SP_002297"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFD2360072.1"
                     /translation="MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPK
                     WLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRAC
                     RFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
                     IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQ
                     TLNVLAFAKKYRPDVLTKTSLMLGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKN
                     HLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFKRDNLGLDV"
BASE COUNT         2715 a         1467 c         1920 g         2683 t
ORIGIN      
        1 agtacagaat ttatacaaga ggtatactga ttcaattacg gagattactt atgaaattat
       61 ggaaagtgtt atgtagtttt gtaattgctg tatgttattt accattagcc tttgcagcat
      121 cacagtctcc tgccggtacc tggacaacca ttgatgataa aaccggagct aagagagcag
      181 tagttaacct taaagtatca aatggtactt taagtggtac tattgtgaag gtatatccac
      241 aaccaggtga taccggaatt tgttctaagt gccctggcgc attcaaggac aaaaaaattc
      301 aagggttgac ttttgtttgg ggattaaaag atgaaggcaa cggcgtctgg agtggtggct
      361 ctatcctcga tcccaaaaca ggaaaaattt ataaggctaa gatgacactg cagggtaata
      421 aactgtatgt cagagggtat tttggagttt ctgctctagg acgtactcaa acctggattc
      481 gataagtagt actgaaggat gtttttatat ataatgtggg atagtgcttt ctcttgtcat
      541 tgaaagcagt tggtactatc ccgcattttg tttttagtgt tatgatcatt tgcaattaga
      601 ttaggaatgt ctttaaataa attgtcttcc ctataaataa attctcaaaa aatattatta
      661 ccatgcttga tttgcaaata tctaaaaaaa cggttgatcc actaaaacgc cccccgcatt
      721 ctgtagaggc tgagcaatcc attattggtg gtttgatgct cgacaatcaa gtctgggata
      781 aggtaagcaa taaattatgc gaaaccgatt tttatcgtac tgagcaccga attcttttta
      841 gagccatttc tgctttagcc aaaaaagatc aaccttttga tgtagtgacg cttcttgatg
      901 ctttaaaaac acgcaacgag ttagatgatg ctggtggaga agcctattta tttgagttgg
      961 ctaataacac accaagtgta gcgaatgtga ccgcttatgc ggatattgtt cgggaaaaat
     1021 cagtccagag gcaactgata gcggtagcaa cagaaattgc tgattccgct tataatcctg
     1081 ctggaagaga agtgcctgag ctattggata tggccgaaac caaagtattt gctattggtg
     1141 aacaaacagc gagtgacggg ggtccagaaa atattaaatc cattttggta cgggcggtag
     1201 agaaaataga tgccctctat cataatggtg atgcgattac aggccttgcg acaggcttat
     1261 ccgatttgga tgaaatgaca tcgggtttac agccttctga cctgattatc gttgctggcc
     1321 gtccatcgat gggaaaaaca actttggtga tgaatatggc agagcatgcg gcaattaaag
     1381 ccggtaagcc agtattggtt ttctctatgg aaatgcctgc tgattccctg gcaatgagaa
     1441 tgatgtcttc cttggggcga atagatcagc atcggattcg tactggtaaa ttagatgata
     1501 atgactggcc acgagtgacc tccgcggtgc atatgctttc tgaagctcct ttgtttatag
     1561 atgatactcc agctttaagt cctggagaaa tgagggcacg tgccagacgt ttagcaaagg
     1621 aacatggttc gttagggctt atcgttgtcg attatttaca attaatgaaa gtgcccgggt
     1681 ttaatgcaga caatagaaca gcggaaatct ccgaaatttc tcggagtctt aaatcgctgg
     1741 caaaagagtt acaggttccc gtaattgctt tatctcagct gaatagaagc ctcgagcagc
     1801 gagcagataa gcggccagtg atgtcagatt tgcgagaatc aggagcaatt gagcaggatg
     1861 ctgacttaat ctgttttatt tatagagatg aagtatataa tgaggacagt cctgataaag
     1921 gcactgcgga aatcattgtt gccaagcaaa gaaatggacc cataggcaaa gtcagggtag
     1981 cctttttggg taagtacaca cgatttgaag atttggcatt taatggttat caaggggtag
     2041 aatagggtgt caagacctac acgtttagtg atagagccca gtgctttatt gcataattta
     2101 tctcaaatca agcatttagc tccaggcaaa aaagttatcg ctatggtaaa agccaacgcc
     2161 tatggctgtg gagtcaggga agttgcccct gtccttgatg ggcgtattga ggcttttgga
     2221 gtagcctgcc ttgaggaggc tctggctatt cgagctttag gtgttgaaac cccgtgcatt
     2281 ctctttcagg gcgtctttag ttctgatgaa ttgtcagtag ccgttgagaa tgatttcgcc
     2341 tgtgttcttc atcacgcaca gcagttggaa tggctaatca agactccgtt gccttacccg
     2401 ataaaagtat gggtgaaggt aaacacaggc atgcacagac tgggatttaa aatccatgaa
     2461 ttgcaaaaag tgatgggggc attgcaaacc tgtacctggg ttgataaaag tatagggtta
     2521 atgactcatt tggcttgcgc agatgaacct catcgtcctg agaatcaaca acagatctct
     2581 ttgtttcagg agatatccat acctgggttt cgccaacgca gtatcgctaa ttcggcggca
     2641 attatttcat tccctgattc gcaggctgat gtggtgaggc ccgggattat gttgtatggt
     2701 gtttccccat ttgcaaatca aaatgcccat gatttaggtt taatccctgt catgcgtttt
     2761 atgtcagcca tcagcgcgat tcatgataac ccatcttttg cccaggtagg ctatggtgga
     2821 acctggaaaa gtgataaacc ttcgcgaatt ggtgttgttg cagcaggtta tggcgatggg
     2881 tacccacggc atatttccga gaaaacaccg gtttgggtta gagggaggga ggtgtctatt
     2941 gttggcagag tttctatgga tatgctgacc atcgatttga cagagcaccc tgatattgag
     3001 atcggcgatg aagttgagct ttggggaaca catgttttag tagagcgcat tgccaaatca
     3061 gcaggtacag taggatacga attattatgt caaatttcgg agagagtacg ttacaaataa
     3121 gttatggata ataactgttc gtggtggtag aatgacaacc gattttgtaa ctctttaaac
     3181 aggtgaactg taatgaaaaa attaattgtt gcatcattat ccctaacatt catgctaggt
     3241 ggctgtgcct caatgaacaa agaaggtgta ggtacaatta ctggtggtgt ggtaggtgga
     3301 ttacttggta gccagtttgg tggtggttca ggtaaggttg cagctgcagg agcaggagca
     3361 ttattaggag cttacctggg tggccaaatt ggaaaaacaa tggataaagt agaccgactt
     3421 gaaatgcaaa gggccctcga aacagctcca acaggtaaag cagttgcatg gtcaaatccg
     3481 gatactggat accgttatac tgtccagcca acgagaactt attataatgc tcaacaacct
     3541 tgccgtgagt acattaccaa agccatgatt ggtggaaaaa cccaacaaat ttatggtaag
     3601 gcctgtcgtc aagctgatgg ttcgtggcgt gttgtgaatt aattaattga ttaagattgt
     3661 tggggaaaac ccaacaatct cattttatta ttctaaccaa ttaagacaga acttacgaaa
     3721 aatccccgtc tctttgttaa aaaatgtttt taattcggtc atttggctta tctaaattcc
     3781 ttcgttaaaa tcattttttg ccttgacctt ggagttttca atatacaaat cctacgataa
     3841 tcttttataa aaatctttcc tgctttcgat taatgagctt aacccctcct tgttttgtgc
     3901 tatatttata cagcgagttg ctaatttagt attaatcagg gagagtgcaa tggctcactt
     3961 aattcatagc gtactgccgg tgccaccaag gaaaatcgct tatattcatc ctgatgatag
     4021 tgtcaaagaa tgtattaaac agatggttga aaaagatata ggtgcattag ttgttttcga
     4081 caatgatgcc cacttgattg gtattgtgag tgaaagagac atacttcgct gttattttca
     4141 taagagtcta agtttggaaa ctgcaaaagt atctgatgtt gtttatacca acgtcacgat
     4201 cttaagtcca catgattcgg tagagaaagc catgcaagtg ataactgaaa caaaaagaag
     4261 acatgtatta atccaggaag aaggtgagct attagctata ttgtcaatag gagacttgtt
     4321 gtaccacgta ctggaagata aattgcgagt gattgagcat ttggaaaatt atattcactc
     4381 ctgaaatata aactcttatt tcagatttat attttttgct ttaaacgtga gtgtctggct
     4441 atttcataca atcgatccta agtgctatag tattcattta caaagaaata taatgaaaac
     4501 catgaagcaa atgattcgat tgacaatgtt acttctgatg ctcagtttta atttatgggc
     4561 aataaattct tcatctccct ggtatacaga agaaaaaggt aaagggcttg taattaacgt
     4621 tgaattgtat ctttcatcta cttgtcctca ttgtcataag gccgatgaat ttttcaaaga
     4681 aattgaaaaa cattatacct ggttgcatgt tgagcgttat attattgata aagataaaaa
     4741 ggctttgaaa cgctttggcg atttattgac agaacttaat cgatatgatt ttgccgttcc
     4801 atccatattt ttttgtaatt cccgttgggt tgggtttgct actgcacaga caaccggaaa
     4861 ggatctgtta aacggcatag aatattgtaa agaagaaatt gtgaaaaatg gaagattgac
     4921 tcctgcgacc gagaatgttt taaacagatg ggggaatgcg agtttgtttg attccaatat
     4981 cacaggaagc ccttccacaa atacatttat ttttgttgta gccataatcg atgcaatcag
     5041 tccatgtgct ttatttacga ttactgcatt tttaggtctt ttattcatga ttgaaaagcg
     5101 aaagctgcaa tttattagcg gttccgtgtt tattttaact gttgcaggtg ttcattattt
     5161 tcaacaggtt tatcctaccc tgttttttga gtcgctttca tggttccgta ttccggctgc
     5221 attggtaggc ctttttgctt tttatttcgc tggccaatat tataaaaagc attctatcca
     5281 accattattt attgttttgg cttttttatt ggctcttaca gtgccaatgt ttcaacaaac
     5341 ctgtttgatg aattggtctt atgtgtttga acaatggtta cataaccaaa atgtatcagg
     5401 agttcagatg gggctatatc aatttgctta tcagcttatc tatatctttc ctttaattat
     5461 cctgatgttt ctctattgga ttttaatgaa aattcatttt ttcaaaaaat ttaagacaaa
     5521 attaactact ataggttttt tatatatttt agctatcgca ctcttattaa tcgtttatcc
     5581 ctatgccttg tctaatttgg ctttatcatt atttttaatt gtctcattgg ggatctccgg
     5641 tctgttactg aattggttta atgcttctaa aatgacatga gggttcaatc ggcataaaat
     5701 aattataaat tctgaatatc tttcatttta tggcatacaa ttaaaaatga gccaggctta
     5761 gaagctgatc gcttatggag aaattctcat catggatgat aataattcag agtttaagca
     5821 tttatttcca atagctgtgg aggtaacaga aggaaagact tacgtatggt gcggttgtgg
     5881 caaaagcaag acccagcctt tctgtgataa agagaattgc ggggataaag cagtctcttt
     5941 tatagcagag ttgactgaag acgtttattt ttgcaattgc aaacaaacca aaaaccctcc
     6001 tttttgtgat ggatcgcatg caaagatttt gcttgaagtg gttaaaaaaa aggcaaaaca
     6061 aatgaacagg aaaggaagtc ttaaatgatt gatcaagaat taaagaaaaa aattgttaac
     6121 acaattagcc aatatgagca caaatgtgac aagatgcaaa gtcaaattaa tacgctcaga
     6181 agtgtgatta atcaattgat tattaatccg attggaatta attatgagtt agatgatcgc
     6241 ctttcgcaat taagaaatta tttagatact gaatttgaac ctgctgtcat agaaaaaaaa
     6301 gtgaaacgtt tggcagaaat actttccaaa atgcaaagga aaaaagcgga aaattctcgt
     6361 ttagtcaatg gactaatcaa acagggagtg gaatccattg gtcgtattgc caataaatct
     6421 caggataaga gggcagttac aaaattacaa aaaatgctgg ataccgagat ggaaagccat
     6481 gcgattttga ttcgttttaa tcaagtctta actcaatgta tttcttctgt gattaaggag
     6541 atggagaagt taccacaaat tacaaaagtc gatacccccc aaaaaagtta tcagtctgag
     6601 attagtttaa aagtaaatga cagcttgcaa caattactta accatttatc tatccctcag
     6661 gacttggatt ccaagagggg agtcataaaa acaaatttgg aacatgcact cactgatgaa
     6721 gagcttagca ctattattga caatttgacg gaattagtcg ttgagtcatt taatgttgag
     6781 cagaatcgat tcaaagggtt tcttcaacag ttaaccgatc agctcaatga ttttgaaatc
     6841 tatttaagag tatcaggaga tgctcgcaaa aaggcaagtg aagacagtcg tttattagag
     6901 tgtgggattc aggataatct tgatcaaatc aagaatcatt tggatacctc taaaacgatt
     6961 gaggaattat cctcaaaggt gagccaaaat ataaaaatga ttggtgacag gttaaaagaa
     7021 tatcgacaca atgaaaaaat tcgagaggaa gaatatgcaa agcaagtatc cgagttgcaa
     7081 gctagattac aggaatcaga gcaaaatgcg gaagaaataa aaaatttgct tgcttttcag
     7141 aaatacagaa ttaaccacga cagcttaaca ggcttgccaa atcgggaatc ttatgatgag
     7201 catatcctgg attcattcca gcgctggaaa cgaagttcaa gcgcattgtc tcttgctgtg
     7261 tgtgacatcg atcattttaa acgtattaat gataattttg gccatcttgc aggagataag
     7321 gttcttaaaa aggtagctat gatttttaaa tcatcaatca gaacggttga ttttatcgct
     7381 cgaataggtg gtgaggaatt tgtgtttatt tttgaacaaa ccagcagcaa ggaagctttt
     7441 ggaattttgg agaaattacg taaattagtt gaagattgtt tgttttatta tcgagacaaa
     7501 aaagtcgatg ttactgtttc ttttgggcta actacggtaa ccgaagaaga tgatattgaa
     7561 tctttattta tgcgggcaga caatgccatg tataaagcta aaaatgcagg aaagaacagg
     7621 gtagaaatat tatagtggaa tatatttcca acaattttta attatactat tttcggagta
     7681 aatccatggt aacagatgat tttcctctga taacgatttt taaatgaact tgatcttatc
     7741 tttgtgattt gggatcatca taactctttg tatttcggat ggcgtcatgg gcaaattgat
     7801 cgatattcca atcgttgtgg aaagtggaca aaaatataag acatctcagg gtgtgacagc
     7861 tattaaagat ggaataaaat catcaggtca agatcatgag cgattgccaa aaccgaagtg
     7921 gcttcgtata gtcaatcata caactccagc ctatagccaa gttaaagagc aagtgcaaaa
     7981 acatcgtttg gcaacagttt gtgaagaagc gaaatgcccc aacatctcag aatgttggtc
     8041 tcatggaact gcaactatta tgttaatggg agcagtatgt acccgggctt gccgattttg
     8101 ttctgtggat acaggaaatc cgcatggttg gcttgatgca gaagaacctg agaataccgc
     8161 agaaactgtg gccttaatga atctggatta tgtggtttta acctcagtta accgtgatga
     8221 tttacctgat gggggggcga atcattatgc caaaaccatt agagcaatca aaaaacgatc
     8281 tccccgaacc aaagtcgaag cgctaacccc cgattttcaa ggaagtgaaa gagatgttgc
     8341 tgtattgtta gatagtggcg ttgatgtgtt tgcgcaaaat gtagaaacgg ttgagcgcct
     8401 gacacacccg gtaagagata atcgagcggg ttatcagcaa accttgaatg tactggcttt
     8461 tgctaagaaa tatcgaccgg atgttctgac caaaaccagt ttgatgttgg gtttgggtga
     8521 aaccgatgag gaaattatac agactatgga tgaccttcga actcatcacg tggatatttt
     8581 gactttggga caatatttgc aaccaactaa aaatcattta cctatagcac gttacgtaac
     8641 tcctgaaact ttttctgaat taaggcaaat tggtctaaaa aagggctttt ttgaagtggc
     8701 ttcaggccca ttagttcgat caagctatag ggctgaccga gtgttcaaac gggataattt
     8761 agggcttgat gtttgaagtt agtga
//
LOCUS       DAXLDT010000121         8748 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXLDT010000121 DAXLDT010000000
VERSION     DAXLDT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.121"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..487)
                     /locus_tag="ACF2SP_002298"
     CDS             complement(<1..487)
                     /locus_tag="ACF2SP_002298"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFD2360073.1"
                     /translation="MSSSDEKIDKPKLCWPGKHLNERVSLPFQTIETINNSEIDHHKS
                     NWDNRLIWGENFLVMSSLLKEFAGKIQLIYIDPPFATGQDFSYTVNIGEHTEAMKIPS
                     ALEVKAYRDTWGKGTESYLQMMYDRLSIMKDLLAENGCLYVHCDWRVNYLLRFILNEI
                     FG"
     gene            complement(818..1780)
                     /locus_tag="ACF2SP_002299"
     CDS             complement(818..1780)
                     /locus_tag="ACF2SP_002299"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFD2360074.1"
                     /translation="MKMSREKLQQESASTGFKMEHLEKVFILMELLEDFTSFPQFKDK
                     FALKGGTALNLFFFNLPRLSVDIDLNYIGSTDRETMMSERPALEKTVVAICERKGFTL
                     DRNPGRHAGGKMVWRYPSALGQMGNLEIDLNFMYRIPLWPIEYKSSCAVGSKQIHNIP
                     VLDSHELSAGKLSALIDRKTGRDLFDAHYLLTKTNLDMAKLRLALVVYSAISRKVDLR
                     QLTPQAISVDITDLKNRLIPLIRKTEIEAMNGISNWAEQMVQECQQAFDQFLPLSSSE
                     HLFLENLLDKGVVEPELITTDTLLIENIKNHPAIRWSAQQHHTN"
     gene            complement(1767..2591)
                     /locus_tag="ACF2SP_002300"
     CDS             complement(1767..2591)
                     /locus_tag="ACF2SP_002300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360075.1"
                     /translation="MSTLISFFEQHPVFTQDEYRQFLLSQGTTNPNTQRELLAYHLKK
                     QHIIRIRRSLFASIPAAFRDVAEEFPVDSYLLAGRITQDAVIAYHSAFDLHGVSYSLH
                     HQCLYLSDQLIRPFKFKEADFIRLPFPNSLLEQDATQMDVLSIDRQGLDVKVTSLERT
                     LVDVLDRPNYGGGWEEIWKTADHISILNLDKVINYAQLLNNATTTAKLGFFLEHFKQQ
                     FGVDESSLKLLEAQKPSGVHYLERGKRESGKLLKRWNLVVPTYILDHSWDEPNEDV"
     gene            complement(2818..4026)
                     /locus_tag="ACF2SP_002301"
     CDS             complement(2818..4026)
                     /locus_tag="ACF2SP_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2360076.1"
                     /translation="MGGKRQQGNELQPPRGVSIRQWETGSAIRIIFSYRGVQCRETLK
                     LEPTKANLKYAERLRGEIINAIALGTFNYADYFPDSKRAKLFGHAIIKATTAELLQDY
                     MAIAERTLEASTYNGYRKVCETHLYPAFGKIPIRELSPVIIRNWITGLNVTVKTVRNI
                     LTPLRNMLDQAMNDGIIERNPLDRLVLSKLLNKKTSKSDWEVDPFDQDEIKAILDAAT
                     GQARNLFQFAFFSGLRTSELIALEWNDIDWLKGTVRVSKAVVLKQEKGTKTKSGVRDV
                     LLLPPAFDALKNQKAFTYLEGSRVFYNPRTNTPWETDGQIRKTCWTHLLKKAGVRYRN
                     PYQTRHTYASMLLSAGENPLWLATQMGHRDTEMIIKHYGRWIPDKSTVAGYQPVNDWN
                     FSISTTISKS"
     gene            complement(3975..4181)
                     /locus_tag="ACF2SP_002302"
     CDS             complement(3975..4181)
                     /locus_tag="ACF2SP_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFD2360077.1"
                     /translation="MKWVKLKKYCQDSGDTSDAVHAKRKRGIWLDGVQCKLGPDGNIW
                     INLIEVEKWVENGNKATNCSLRAV"
     gene            complement(4212..4451)
                     /locus_tag="ACF2SP_002303"
     CDS             complement(4212..4451)
                     /locus_tag="ACF2SP_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360078.1"
                     /translation="MDQQVKLNIYKGMIQYIINSTHYTLKNIADLSDTHIDNIRSIYC
                     HNAVPNSFKSEVQLMKLYQIVLEISTNPKNLALAG"
     gene            4620..5267
                     /locus_tag="ACF2SP_002304"
     CDS             4620..5267
                     /locus_tag="ACF2SP_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360079.1"
                     /translation="MTTNILSESNNLQLAQTLTDLIERENKLTPAALARKLGIPTNKI
                     TRILNGDVTDPKASTLLQIANYFGITIEQLLGLESIVREGTSITEPLAQSLPIYEFSH
                     PTRMTNEWYRWSTNEIDGDYFALAIDTDLYEPTFPQNSLLIVNPDMMPDDRSYIVVKR
                     NDSPQHCSIKKYVLEGTQGYLYPINPKLPVELFDRNLYTVAGVILEVHQKLRSKK"
     gene            5284..5643
                     /locus_tag="ACF2SP_002305"
     CDS             5284..5643
                     /locus_tag="ACF2SP_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360080.1"
                     /translation="MEKWNQGNRFLRFLYQHLYLRFNGQIREPYLIRIDNIYTDPDSN
                     ELTVSFHIANKRVNQDMSVAEFVKTDMIYLVDPRIVFDMGQQFGSHSEKLIVAQKTEP
                     SLKKRCVSTLKRVFIDE"
     gene            5636..7063
                     /locus_tag="ACF2SP_002306"
     CDS             5636..7063
                     /locus_tag="ACF2SP_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HFD2360081.1"
                     /translation="MNNSIGKSKTPLQYKVFHTLLGFAVFFSLITVPAEAQVIQIHGI
                     LTSSELLWWPVVFFVLRLIHGVYGFAYLRHAVYAVLLFHAIYVLFLKFAIWLPASSFW
                     KMQEPYTQVLGRDFVYLIKSSLFLWVCALLPIRFASSANHKYYGYIFWVSLVAFCFLD
                     MGWLNMHKNTPDTQIVVPLLIFGLLNIFYNWLSVVIARIEHIESPIQSDRHLLKFQLP
                     QVLKNDGNTFKYHHMLFCSSIVFFIASKTMAAKFISIGFLTINVGGIVFSLAYLAADM
                     MTDVYGIERTKQMVLFVIFCNLLFVFDVWVTNMLAIGENEPYRAILHNQARMFIASAT
                     AFFLGMTINSTVISLIKSRQRKRGISLKKEFITTVWTRIATSSAFGIIIDVSLFSLVA
                     FYGIVPTEKLASVIVFEDAYKISYEVFLAPVSILMIYFLKVKEKVDIYDELSNLNPFR
                     IDTNYKVSANKFAENYMKPAERNDG"
     gene            7056..7517
                     /locus_tag="ACF2SP_002307"
     CDS             7056..7517
                     /locus_tag="ACF2SP_002307"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFD2360082.1"
                     /translation="MDKYIYDFMAATIEVAATNPMAPYGAIIVYDDKEILLKSVNNAH
                     NNPLMHGELSVIHRLFENGFDGDVSRLSLYTTAEPCPMCAAAIYWAMIPKVVYGSSIS
                     FLHELFGRQIQVDAKEILSKTPNFYECQLLGGVMEKNCNQLFIEAKKLREL"
     gene            complement(7544..8353)
                     /locus_tag="ACF2SP_002308"
     CDS             complement(7544..8353)
                     /locus_tag="ACF2SP_002308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFD2360083.1"
                     /translation="MKLQIFDVEHGACALLTCINGTRLMIDCGHNANSGWKPGSYLRS
                     QGVTHLDMLAITNYDEDHVSGLPDLLEQVHVNWLWRNPSVSATDIKNMKAKNGMGYGI
                     ESLVNILPTYKYTGWQPPEFSFVERRGFYAPYPTFVDTNNLSMAIHLNIAGINFLFSG
                     DLESGGWNTLLKDQSFRNVIANTDIFIAPHHGRESGIYPQIFEEYQCFPHYIIMSDKS
                     HMYDTQKTLDYYSSKARGGFFRGKHRRVLTTRKDGSITFNFNFLKGECLVA"
     gene            complement(8357..>8748)
                     /locus_tag="ACF2SP_002309"
     CDS             complement(8357..>8748)
                     /locus_tag="ACF2SP_002309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360084.1"
                     /translation="GHRAFSHFVFSDPRINLDSLRSKLGEFPDNPRSQNLLWYSLLKK
                     HESNITVKEAHQYFLLFRDATSMTLIIFLLFFASTFFYEIHMAKFVFLMLLGEYLLLM
                     IASRNMANGLVKNVLSLESNSPAIKGD"
BASE COUNT         2539 a         1758 c         1659 g         2792 t
ORIGIN      
        1 ctccaaaaat ctcatttaaa ataaacctta atagataatt aaccctccaa tcacaatgaa
       61 cataaagaca gccattttca gctaataaat ctttcattat ggataatctg tcatacatca
      121 tttgtaaata agactctgtt cctttccccc atgtatctcg atatgctttg acctcaagag
      181 cagatggtat tttcatagct tctgtatgct caccaatatt tactgtatat gaaaaatctt
      241 ggcctgtagc aaaaggaggg tcaatgtata taagttggat tttgccagca aactctttaa
      301 gcaatgagct cattactaaa aaattctcgc cccaaataag acggttatcc caattacttt
      361 tatggtgatc aatctcgcta ttatttattg tttcaatcgt ttgaaatggt aatgagaccc
      421 gttcgttgag atgtttccca ggccaacaaa gttttggttt atctattttt tcatcagagc
      481 tggacattta ttgttccaat tcacccacca gataaagtta atgtcggtaa tcagaaaaat
      541 tactttagaa tccttaactg ggagtaataa gggtcagact tgtcctttgc ctcttgcata
      601 taatgttgtt ttataattct gctaattctt gagtagtgaa gcactttaat cccttgattt
      661 ataagggttt atcatggtcc ttggtggtca ggaatggttt taatgattgg tggaggcggc
      721 gggaatcgaa cccgcgacaa tttttggtaa cttgttgtta ttactggggt ataataagtg
      781 cctccgtttt ttgggcagat aatgggtagt ttctctctca atttgtatgg tgttgttgtg
      841 ccgaccatcg aattgccgga tggttcttaa tattctcgat aagaagcgta tctgtggtta
      901 ttaactctgg ttcaacaacc cctttgtcca gcaaattttc taaaaataaa tgctcgctac
      961 tactcaatgg caaaaactgg tcaaacgctt gctggcattc ctgaaccatc tgttctgccc
     1021 aattggaaat gccattcatt gcttcaattt ctgttttcct gattaacggt attagcctat
     1081 tctttaaatc agtaatatct acagaaatag cttgcggggt taactgtcgc aaatccacct
     1141 tgcgactaat cgcagaatat acaaccagtg ccaggcgtag cttggccata tccagattgg
     1201 ttttagtcaa aagataatga gcatcaaaca ggtctcttcc cgtttttctg tctattaatg
     1261 ccgataactt accagcagat aattcatgcg aatccaaaac agggatgttg tggatctgtt
     1321 tgctgccaac agcacagcta gatttatatt cgatcggcca taaggggatg cgatacatga
     1381 aattaaggtc tatttctaaa tttcccattt gccccaaagc actaggataa cgccaaacca
     1441 tcttaccacc tgcatgcctg cctgggtttc tatccaaagt aaagccttta cgctcacaaa
     1501 tagcaaccac cgttttttca agtgctggtc gctcagacat catggtttcc cgatcagtac
     1561 ttccaatata attcaaatca atatccaccg ataatcgggg taaattgaaa aagaaaagat
     1621 taagtgcagt gccacctttc aacgcaaatt tgtctttaaa ttgtgggaaa gaggtaaaat
     1681 cttcaagtaa ttccattaaa atgaaaactt tctcaagatg ttccatttta aatccagtcg
     1741 aggctgattc ttgttgtagt ttttctctag acatcttcat ttggttcatc ccaagaatga
     1801 tcaagaatat aagtaggaac gacaaggttc catcgtttca agagctttcc agactcacgt
     1861 ttacctcgtt ccagatagtg aacaccactg ggtttttgcg cttccaaaag ttttaagctg
     1921 ctttcgtcca caccaaattg ctgtttaaag tgctcaagaa aaaagccaag tttggcagtt
     1981 gtcgttgcgt tattcaatag ctgcgcatag ttaataactt tatccagatt taagatagaa
     2041 atatgatcag ctgtcttcca gatttcttcc cagccaccgc cataattagg gcgatcaagc
     2101 acatcaacta atgtcctttc aaggcttgtc acttttacat ccaagccttg cctatcaata
     2161 gataggacat ccatttgtgt ggcatcttgt tctaataggc tattgggaaa tggcaagcgt
     2221 ataaaatcag cttccttgaa tttaaaagga cgaatcagct gatcggacag gtataaacat
     2281 tggtgatgca gggaataaga gactccatgt aaatcaaaag cggaatgata ggcaatcaca
     2341 gcatcttgcg tgatgcggcc tgcaaggaga tatgaatcta ctgggaattc ctctgcgaca
     2401 tcacgaaaag cagccggtat actagcgaag aggcttcgcc ggatcctaat aatatgttgt
     2461 ttttttagat ggtaagctaa tagctccctt tgagtattag gatttgtagt accttgtgac
     2521 aataaaaatt gcctgtactc atcttgagtg aagactgggt gttgttcaaa aaaactaatt
     2581 aaagtgctca tttgattttg acatttattg tcaataatac taattatttt agttttattg
     2641 actgtttttg tcaattaaaa tttgacatta attgctgaag ttactaattt atttagtaat
     2701 tttgatttat tttgtcaaat tgattgtcta tataaaaaaa taaccctacc atgcaatctg
     2761 gttgtgaaac ttaacgaaat atgtagagtt ttacaaccag actgcataaa ttaattctta
     2821 gctcttgctg attgtagtac taatactaaa attccaatca ttcacaggct gataaccagc
     2881 aacagtagac ttatcaggta tccatcgccc atagtgtttg ataatcatct ccgtatccct
     2941 atgtcccatc tgtgtggcca accacaatgg attttcaccg gcagaaagca gcatggaagc
     3001 atatgtatgc cgcgtttggt aaggattgcg atagcgcaca cctgcttttt tcaaaagatg
     3061 tgtccagcaa gttttgcgaa tctggccgtc tgtctcccac ggagtattgg ttcttgggtt
     3121 ataaaaaacc ctagatcctt ccaaataggt aaatgctttt tgatttttta atgcatcaaa
     3181 agctgggggt agtaataaca catccctcac ccctgatttt gttttagttc ccttttcctg
     3241 ttttagaact accgccttag atacacggac agttcctttt agccaatcaa tatcattcca
     3301 ctccaatgca ataagctcag atgttctcag acctgaaaaa aaagcaaatt gaaacaagtt
     3361 tcttgcctgt ccagttgctg catccagaat tgccttaatt tcatcctgat caaatgggtc
     3421 aacctcccag tcacttttac tggttttctt gttgagcaat ttagaaagca ccaatctatc
     3481 aaggggattg cgttcgataa ttccatcatt catagcttgg tccagcatat ttcgcagggg
     3541 agtcaaaata ttgcgaaccg tttttaccgt aacattcaac cctgtaatcc agttacggat
     3601 aatcacaggc gataattcac gaattggtat tttgccgaaa gcaggataca aatgagtctc
     3661 acacactttt ctatagccat tgtaagtact ggcttccaaa gttctctcag caatggccat
     3721 gtaatcctga agcaactcag ccgttgtggc tttgatgatt gcatggccaa atagctttgc
     3781 acgtttggag tcaggaaaat aatccgcata gttaaatgtt cccagtgcga ttgcgttaat
     3841 gatctcaccg cgcaaccgct cggcatattt tagattcgct ttcgtaggtt ccagtttcaa
     3901 agtctcacgg cactgaactc ctcgataact gaagataatc cgaatagcag atccagtttc
     3961 ccactgacgg atacttacac cgcgcggagg ctgcagttcg ttgccttgtt gccgttttcc
     4021 acccattttt caacctcaat taaattaatc catatgttgc catctggtcc taatttacat
     4081 tgtacaccat cgagccagat accgcgtttg cgtttggcgt gtactgcatc agaagtatct
     4141 cctgaatcct gacaatattt tttaagtttt acccatttca taatcaaaac ttccttcttt
     4201 atttatgaaa gttaaccagc caaagcgagg ttctttggat tggtgctgat ttcaagaaca
     4261 atttgataga gcttcataag ctgcacctct gacttgaaac tgtttggaac cgcattgtga
     4321 caataaatag aacgaatatt gtcgatgtgc gtatccgaca aatccgcaat attcttcaag
     4381 gtgtaatgtg tcgaattgat gatgtactga atcattccct tatagatgtt cagttttact
     4441 tgctgatcca tatgcaaaca ctccttactg caaggcaaat ctactgcgct gctgatttaa
     4501 ttaaaatcat gaccaatgac taagtgatat tcccataaaa tatggtaata taagttttac
     4561 atcggtgtaa ttcgttacat caatgtaaat tattacatct atgtaacgaa agtcaaggta
     4621 tgacgacaaa tattttaagt gaatctaata atctccaatt agcgcaaacg cttacggatt
     4681 taattgaacg tgagaataaa ctgacgcctg cagcacttgc acgaaagcta ggtataccaa
     4741 ccaacaaaat cacaaggatt ttgaatggtg atgttaccga tccaaaagcg agtaccctgc
     4801 tgcagattgc aaactatttt ggaattacta ttgagcagct tcttggttta gaatccattg
     4861 ttcgtgaagg aacatcgatt acagagcctt tggcgcagtc tttacctatt tatgagttca
     4921 gccatccaac tcgaatgacg aacgaatggt atcgctggtc aaccaatgag attgatggtg
     4981 attactttgc tctcgcgata gatactgatt tgtatgagcc tacatttccg caaaactcat
     5041 tactgattgt taatcccgac atgatgccgg atgatcgctc gtacattgtt gtgaagagga
     5101 acgatagccc ccagcattgt tccattaaaa aatatgtttt agaaggaact caaggctatt
     5161 tgtaccccat caatcctaaa ttaccagtag aattgttcga tagaaattta tacactgtcg
     5221 ctggtgtgat tcttgaggtg catcaaaagc tacgctccaa aaaataagtt gtaggatgta
     5281 aacatggaaa agtggaatca aggaaatcga tttttacggt ttttgtatca gcatttgtat
     5341 ttacggttca acggacaaat cagggaaccc tatcttattc gcattgataa tatctatacc
     5401 gatccagaca gcaatgaatt aaccgtttcc tttcatattg ccaataagcg tgtgaatcag
     5461 gatatgtctg tggctgagtt tgttaaaacc gatatgattt atctggttga ccctcgcatc
     5521 gtgtttgata tggggcagca gtttggctct cattcagaaa aactgatcgt tgcccaaaag
     5581 acggaaccta gcctaaagaa gagatgcgtc tccaccctca agagggtttt catcgatgaa
     5641 taattccatt ggtaaatcca aaactccctt gcagtataaa gtattccata cgctattggg
     5701 ttttgcagtc ttcttttctt tgattaccgt gccagcagaa gcacaggtga tccaaatcca
     5761 tggaatactc acatcctcag aattactgtg gtggccagtc gtcttttttg tgttgcgtct
     5821 tattcatggt gtttatggct tcgcatatct cagacatgct gtgtatgcag tattgttatt
     5881 tcatgctatt tacgttttgt tcttgaagtt tgccatttgg ctgcctgcct cttcattctg
     5941 gaaaatgcaa gaaccttaca cgcaggtgct gggtcgggat tttgtatacc tgataaaaag
     6001 ttctttattt ttatgggtat gtgcgcttct cccgatacgt tttgcgagtt cggcaaatca
     6061 taaatactac gggtatatct tctgggtatc tttggttgcg ttttgctttt tggatatggg
     6121 ctggttaaat atgcacaaaa atacccccga cacccagatc gtggttcctt tgctcatctt
     6181 tgggttgctt aatatttttt ataattggct ctctgtggtg atcgcgagaa ttgaacacat
     6241 agaaagccca atccagtcgg ataggcatct tttaaaattt caactgccac aagtcctgaa
     6301 gaacgatggc aatacattca aatatcatca catgctgttt tgctccagta tcgtcttttt
     6361 tatcgcatcc aagaccatgg ctgccaaatt catttctatt ggatttttga ccatcaatgt
     6421 tgggggtatt gtcttttcac tggcctacct tgctgcagat atgatgacgg acgtttatgg
     6481 aattgagcgc accaaacaga tggtgctatt tgttattttt tgcaatctac tgtttgtttt
     6541 tgatgtttgg gtaaccaata tgctggcgat tggagagaac gaaccgtacc gagcgatact
     6601 tcacaatcag gcgcgaatgt tcatcgcttc tgccaccgcc ttctttctgg gtatgaccat
     6661 caattccacc gttatttcac tcatcaaatc cagacagcgc aaacggggta tttccctgaa
     6721 aaaagaattt atcaccaccg tttggactcg aatcgccacc tcatcggcat ttggaatcat
     6781 catcgatgtg agcttgtttt cattggtggc attctatggg attgttccaa ctgaaaaact
     6841 ggctagcgtc attgtttttg aagatgccta taaaatatcg tatgaggtct ttctcgcgcc
     6901 tgtgtccatc ctgatgatat atttcctcaa agtgaaggag aaggttgata tttatgacga
     6961 actatccaat ttaaacccat tccgaattga taccaattac aaggtcagcg cgaacaagtt
     7021 tgctgagaac tatatgaaac cagcggagcg taacgatgga taaatatatt tatgacttta
     7081 tggctgccac cattgaagtt gctgcgacta accctatggc tccctatggt gcgattattg
     7141 tttacgatga taaggaaatc cttttgaagt cggtaaacaa cgcacataac aatccattga
     7201 tgcatggtga attatcagtc attcatagac tctttgagaa cgggttcgat ggcgatgtga
     7261 gcaggttaag tctttatacc actgccgaac cttgccccat gtgtgctgcg gcaatttatt
     7321 gggcgatgat tcctaaggta gtttatggga gttctatttc atttttgcat gagttgtttg
     7381 gtcggcaaat tcaggttgac gcaaaagaaa ttttgtcaaa aactcctaac ttttatgaat
     7441 gccaattgct tggcggagtt atggaaaaaa attgcaatca gttgtttatt gaagccaaaa
     7501 aactgcgaga attgtagatt gtgtctccat cacagaagta acttcaagct acaagacatt
     7561 ccccctttaa aaaattaaaa ttaaaggtta ttgaaccatc ctttcttgtc gttaaaactc
     7621 gacgatgttt ccccctaaaa aaaccaccgc gtgctttact agaatagtaa tctagagttt
     7681 tttgtgtatc gtacatatgg cttttatcgg acattataat atagtgaggg aaacactgat
     7741 attcttcgaa aatttgggga taaatcccac tttctcgtcc atgatggggt gctataaaaa
     7801 tatctgtgtt cgcaatcaca ttccgaaagc tctgatcttt taacagagta ttccatcctc
     7861 ctgattctaa atcgcctgaa aacaaaaaat ttattcctgc aatattaaga tgaatggcca
     7921 tgcttagatt attcgtgtct acaaaagtag ggtaaggagc ataaaaaccc cgtctttcaa
     7981 caaatgaaaa ctcaggcggt tgccatcctg tatatttata tgtcgggaga atatttacta
     8041 aactttcaat cccatatccc attccatttt tggctttcat attttttata tctgttgctg
     8101 aaactgatgg attacgccat aaccaattaa catgtacttg ctcaagtaaa tcaggtaatc
     8161 cactcacgtg atcttcatcg taattagtta tggctaacat atcgagatgg gttactcctt
     8221 gactgcgcaa gtaagaacct ggtttccagc cactattagc gttatgtcca cagtcaatca
     8281 ttagtcttgt gccattaatg catgttaaga gtgcgcatgc accatgttct acatcaaaaa
     8341 tttgtaattt cactttttaa tcccctttaa ttgctggaga gttactttcc agagaaagaa
     8401 cattcttcac taatccgttt gccatattgc gagaagcaat cattaaaaga aggtattcgc
     8461 caagcagcat tagaaaaacg aacttagcca tgtgtatttc gtaaaaaaaa gtgcttgcga
     8521 aaaatagcag aaatatgatt aaagtcatgg aggtggcatc ccgaaataaa aggaaatact
     8581 gatgggcttc tttaaccgtt atattagatt catgtttttt taataagcta taccataata
     8641 aattttggga gcgtggatta tctggaaatt cccctaattt gcttcgtaag gaatctaaat
     8701 taattctagg atcactaaaa acaaaatgtg aaaaggcacg atggccag
//
LOCUS       DAXLDT010000122         8721 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXLDT010000122 DAXLDT010000000
VERSION     DAXLDT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.122"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..875
                     /locus_tag="ACF2SP_002310"
     CDS             <1..875
                     /locus_tag="ACF2SP_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HFD2360085.1"
                     /translation="AIGVYAVLLFAYNMLINRAGQTLYGGAPAMGSAYPTVEKVNAVP
                     GGSGDINNNPSASEQVMKGMIIRNANITLEVNDPAKVIDEIMQMAEREGGYVVNSNLR
                     QDSFYVGGNIAQISIRIPSNRLNNVLQNLKSLAVKVIEESITSEDITRQYVDLESELK
                     NYRVAKDQLNKIMQGAKNTSDVLAVYQQLSETQRKIDVIEGQIKYYKESVAYSLITIY
                     LQTNPAIKNAQNSTWQISEVVRKSYQALIEHLRQFTYGFIEFVILFVPMILLWGIIAL
                     LVFWIGKAVYSRFK"
     gene            complement(982..1179)
                     /locus_tag="ACF2SP_002311"
     CDS             complement(982..1179)
                     /locus_tag="ACF2SP_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360086.1"
                     /translation="MLKTLGGATRTAALDTARVTATTTVVLGGMYLWDRVAKNYGLPN
                     KENSKPISLDLGNDNYMGPKV"
     gene            complement(1429..2403)
                     /locus_tag="ACF2SP_002312"
     CDS             complement(1429..2403)
                     /locus_tag="ACF2SP_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1137 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360087.1"
                     /translation="MIQRGFTMQERREKQGNKSPYLLTPYEMANLVFKTGAISFTVSA
                     GTQPFQFLLNKLQFPQSGTPSGLSGGLFRGMYRGFLPYAIAGQKRGAVAVTHKQTNKA
                     TEEEEFEAPVRQRWLGTVLFSQADLLVSNGLSGKARLQNVGVINAENFKWSLSNFWKL
                     TTVNWGSRSFAGGVNFALIGFAGDYVSSFYKFDKDLYNKILGGATSGVIATLFTTVPN
                     AYADSKLLQTKVAENKHLVTVSPYTMFGQMKSHVKAVGLKEAFMTFFKVGYLQQVAVR
                     APQAAITFALIFGMDEYLGPQPLKKVWPGRVEIEELESETPSPSPTKK"
     gene            complement(2662..3684)
                     /locus_tag="ACF2SP_002313"
     CDS             complement(2662..3684)
                     /locus_tag="ACF2SP_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFD2360088.1"
                     /translation="MKIFSLLLALLTFKVFSNPVVNVYAWGGEIPKKVIHQFEQESGI
                     KVNFSTYDSNETMYAKLRASQKTIYDVILPSAYFVERMQKQGMLQLLDHKQLSNLHNL
                     DEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKNWQGLWDKKWRNQLMLLDDS
                     REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGS
                     AWNGDAFKAYTENKAIEFIYPQDGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSA
                     QIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKILRKGYFQRDIGEETLALYNQYWE
                     QLKLAF"
     gene            complement(3681..4448)
                     /locus_tag="ACF2SP_002314"
     CDS             complement(3681..4448)
                     /locus_tag="ACF2SP_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFD2360089.1"
                     /translation="MKTMTQRLFLSLVYAFLYIPIAILVLYSVNDAKFSLQWHGFSLR
                     WYKELFHDRGLWSAVFNSMTLGLTSSLIATTMGLLASINLFLFRTRHRQILYTLLLLL
                     IIIPDIVLGVALLIFFNITQVPLGFLSLLVAHITFSIPFVILTINSRINTLNPNIYFG
                     ALDLGASRYFALTRVLLPLLWPAVLSAFLLSFTLSFDDVIISYFVAGPDFTILPLTIY
                     SLVRAGVTPELNALCSITFGLSMILVIISHRLSGKLI"
     gene            complement(4445..5269)
                     /locus_tag="ACF2SP_002315"
     CDS             complement(4445..5269)
                     /locus_tag="ACF2SP_002315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2360090.1"
                     /translation="MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPL
                     TLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIP
                     FWTSSLIRTYALIAILKYKGVINALLLKLHLIDAPLSLLYTNFAVVIGLVYNLFPFMV
                     LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYI
                     PNVLGGARSILLGNLIQNQFLVLSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR"
     gene            complement(5259..6353)
                     /gene="potA"
                     /locus_tag="ACF2SP_002316"
     CDS             complement(5259..6353)
                     /gene="potA"
                     /locus_tag="ACF2SP_002316"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFD2360091.1"
                     /translation="MTTPLIEIRQIYKSYGNTPILNNVSLNVNHGEFLTLLGPSGCGK
                     TTLLRLISGFEQPTQGEIFINGQCVNQLPPQKRDVHTVFQSYALFPHLSVFENVAFAL
                     RCKKTSNQEIKERVFDALKLVQLELLAERNVKQLSGGQQQRVAIARAIINRPQVLLLD
                     EPLSSLDYRLRKAMQSELKQLQKTLNMTFIFVTHDQEEALSMSDRIVVFNHGHIEQIG
                     TPKAVYETPANLHVAMFIGEANIFDIQVHTVKDQDIVTNIEGIQLSCKNTGNYQVNEW
                     LHLIVRPEDIRVWSLSEVEKTEGMLPGRIVDIIYKGSTVDLKVELSSGKIINASEFFD
                     EDDDKLEYTLHETVWVQWLPGWEVLLPYEG"
     gene            complement(6522..6815)
                     /locus_tag="ACF2SP_002317"
     CDS             complement(6522..6815)
                     /locus_tag="ACF2SP_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFD2360092.1"
                     /translation="MEEKQYVVYILASKAYGTLYTGITSNLVQRIYQHKKGLAEGFTK
                     RYNVHRLVYYEIHTDVYEAITREKRIKKWNRQWKINLIEQKNPQWLDLSIGLC"
     gene            complement(7015..7899)
                     /locus_tag="ACF2SP_002318"
     CDS             complement(7015..7899)
                     /locus_tag="ACF2SP_002318"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2360093.1"
                     /translation="MTKTDKKIVVVTGCSKTSGVGYNLVCEMLKRGHQVIATVRNLET
                     SQINKANLPNSSNLDVKILDLCDENSIDRFIKEVLSQYQYIDALVNNAANVAIGPVES
                     TTTQDVLTTFQTKVFGPLSLIRGFIPVMRERKKGLLTTTSTIFTSVPVSIPGVAIYFS
                     ALAAFERLQESLAIELAPWNIKVINFQPGPIATELTRFEGSRSDIVEKYYKNFTEQAY
                     QWFHQNTVFQNAAEVAKVFAEVIDMENPDLYYQSNLFGKQFVAKNKKDTTGREGMNEL
                     LEHFKNLSHDNPDDWKIQ"
     gene            complement(8242..>8721)
                     /locus_tag="ACF2SP_002319"
     CDS             complement(8242..>8721)
                     /locus_tag="ACF2SP_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2360094.1"
                     /translation="VMIMPLTPPASPKTERRSEEDNQQTNQQGNEQGNQQPRSSILTL
                     FGFTPNALVMPCPHGHGHFHIMDIQADDTLVFATMNGSPEIMSLLALQFLMQNINLNL
                     DDILAAETDAKEAEEKEEPELSSSESVNEEKEESEFSSSESENEEKEENEELNRFTM"
BASE COUNT         2733 a         1590 c         1677 g         2721 t
ORIGIN      
        1 tcgctattgg agtttacgct gtccttctat ttgcgtacaa catgctgata aatcgagccg
       61 gacaaacatt atatgggggc gctccagcaa tggggagcgc ctatcccact gttgaaaaag
      121 tcaatgcggt accaggtggt tctggtgata tcaacaataa tccttctgct tcagagcaag
      181 tcatgaaagg aatgataatt cgtaatgcga atattactct ggaggtcaat gatccggcaa
      241 aagttatcga tgaaattatg cagatggctg agcgagaagg aggatatgtg gttaactcta
      301 atttaagaca ggatagtttc tatgttggtg gcaatatcgc tcaaatcagc atcagaatac
      361 caagtaatcg tttaaacaat gtattgcaaa atttaaaatc tttagctgtt aaagtgattg
      421 aggaatcaat tacgagtgaa gacatcaccc gacaatatgt tgatcttgaa agtgagctga
      481 aaaattacag ggttgctaaa gatcaattaa ataagattat gcaaggtgcc aaaaacactt
      541 cagatgtgct tgctgtttat caacaactga gtgaaacgca gagaaaaatt gatgttatag
      601 aagggcagat aaagtactat aaagaatctg ttgcctattc actcattaca atttacctgc
      661 aaaccaatcc ggcaataaaa aatgcacaaa attcgacatg gcaaatatca gaagttgtta
      721 gaaaatctta ccaggcatta atagagcact taagacagtt tacctacgga ttcattgaat
      781 tcgttatctt atttgtccca atgattttat tgtgggggat tattgcctta ttagtatttt
      841 ggataggtaa ggctgtttat tctcgtttta aatagtagga tttacagtat tagtaggatt
      901 tacagtatat ctcagatttt ggtttaagag agagctaaat actcccaaca ggatagttgg
      961 gagcggaaat aatcttttag tttaaacttt aggacccata taattgtcat tacccagatc
     1021 aaggcttatt ggtttggaat tttccttgtt aggtagccca taatttttag ctactctgtc
     1081 ccataaatac atgccgccta gaaccactgt tgttgttgct gttactctcg cggtatcaag
     1141 tgctgctgtc ctggttgctc cgcctaaggt ctttaacata catttcccaa agaatgataa
     1201 catatttacc gtcctgatta gttaataata atcctgctaa ttattcatca ttcattctaa
     1261 atgaatttct gcctaatgca atacatttcc ctgtttatat gattaaaatc aaatcaacca
     1321 tgtctttttt taacgtcatg tggattaaat aagtacaatt tataccgtaa aacgtattgt
     1381 tgtttgttaa ggcacaatga tgaaaaataa catccttgtt actcttaatt atttcttggt
     1441 aggggatgga gatggagtct ctgactcaag ctcttcgatt tcgactcgtc caggccaaac
     1501 ctttttcaaa ggctgaggac ccaaatattc atccatacca aaaatgaggg caaaagtgat
     1561 agctgcttga ggagctctta ccgctacttg ttgcaaatag ccaactttaa aaaaggtcat
     1621 gaatgcttct ttaagaccaa ccgcttttac atgtgatttc atttgaccaa acatggtata
     1681 gggtgatacc gtaaccaaat gcttattctc agccactttg gtttgcaaca atttactatc
     1741 cgcataagca ttagggacgg ttgtaaataa tgttgcaatg acaccagaag tagcgccgcc
     1801 taatattttg ttatacaagt ctttatcaaa cttataaaat gaactgacat aatcccctgc
     1861 aaatcctatc aatgcgaaat tgactccacc agcaaaagag cgcgaacccc agttaacagt
     1921 agtcaatttc caaaagttgg acaaggacca tttgaagttt tcagcattaa taaccccaac
     1981 attttgcaac ctggccttgc cacttaaacc gttactgact aaaagatcag cttgagagaa
     2041 gagtactgtt cccaaccaac gttgacgaac tggagcttca aattcttctt cttctgttgc
     2101 tttgttggtt tgcttatggg ttactgctac agcaccgcgt ttttggcctg caatagcata
     2161 aggaaggaat ccacggtaca tgcctcggaa aagtcctccg ctcaatccag atggggttcc
     2221 agattgtgga aattgcaatt tgtttagtaa aaattgaaat ggttgagttc ctgcagaaac
     2281 agtgaaagaa atggctcctg ttttaaaaac taaattggcc atttcatagg gtgtcaacag
     2341 atagggtgat ttattaccct gtttctctct acgttcttgc attgtaaatc ctctttgaat
     2401 cacttaaatc aatgtgctgt aatgataaca atttattatt aagcaaatat taactggtgt
     2461 gaaaaaaaat attaagtatg aaaataatat gtaaaaagtg agacaaataa gaccataaaa
     2521 tagattgaat ttatacaaat taagcataat tattttcttt ttgtgtataa aatattcaat
     2581 aaaccttgct ttctgaattt taattcgttg agttaatgaa gtacttcatc tggatattta
     2641 gcgctacttc acaacagaaa actaaaaagc aagttttaac tgttcccaat attgattata
     2701 aagcgctagt gtttcttcgc cgatgtcgcg ttggaaataa cccttcctta gtattttgtc
     2761 tggcggataa accacagggt tattttttat aggtaatggc aataaagctc ttcctgcggc
     2821 attggtaatc gcatgtcctt cctttagtgc aatttgagcg cttgtttccg gtttcagaat
     2881 aaaattaatg aattgatacg cctctttcag gtgtgggggg tttttaggga tagccaaaca
     2941 atctacccaa atcacaaaac catcttgagg ataaataaac tcaatggctt tattttctgt
     3001 atatgctttg aaggcatcgc cattccatgc tgatccaata aaagcatcct catctatcat
     3061 gatggcttga atactatcgc tggcaaataa cttaatatta ggtaccaatt gcaataagtg
     3121 ttcataggct gcttttatgt ggtttggatt ggtatcattg ggatcaaacc ctaaactcat
     3181 cagagctatg gcaaatattt cgcgtgaatc atcaagtaac attagctgat tgcgccactt
     3241 tttatcccat aagccttgcc agttttttgg cgggttcgat acggaattat gattaaagaa
     3301 aatcccggtt gctccccaga tgatgggtat actataacgg ttacccggat cataatcatt
     3361 tttaacaaat tgttcatcca gattatgaag attcgaaagc tgtttatgat cgagtaattg
     3421 caacatgcct tgtttttgca tgcgctctac aaaataagcg gaagggagga tgacatcgta
     3481 aatggtcttt tgactggctc ttagcttggc atacatggtt tcattgctat cataagtcga
     3541 aaaattgact ttaattcctg actcttgttc gaattgatga attacttttt tagggatttc
     3601 acctccccag gcatagacat tgactactgg attggagaat accttaaatg taagaagagc
     3661 gagcagcaat gaaaaaattt tcatatcaat ttacctgaca agcgatgaga gataataacg
     3721 agaatcatcg ataaaccgaa cgtaattgaa cacaatgcat tgagttcagg cgtgacgcca
     3781 gcacggacta aagagtagat ggtcaaaggc aggatagtaa aatcagggcc tgccacaaaa
     3841 tagctgataa tgacgtcatc aaatgacaaa gtgaaactta ataaaaaagc gcttaaaacc
     3901 gcaggccaca ataaaggaag caagactctc gttaaagcaa aataacgact ggcgccaaga
     3961 tcgagggcgc caaaataaat gttgggattt aaagtgttta ttcgtgaatt aatggtcaag
     4021 ataacaaaag gaatggagaa tgtaatatgg gcgactaaaa ggctcaagaa gccaagcggt
     4081 acttgggtaa tattaaagaa aattaataag gcgacaccca agactatgtc aggaataata
     4141 ataagtaata gtaataaggt atacaggatc tggcggtgtc ttgtacgaaa taaaaataag
     4201 ttgattgagg ccaatagccc cattgttgta gcgataagag aggatgttag gcccagggtc
     4261 atagaattga aaacagcaga ccacaagcct cgatcatgaa atagttcttt gtaccatcgc
     4321 aaagaaaacc catgccattg taatgaaaat ttggcatcat ttactgaata gagcaccagt
     4381 attgcaatgg ggatataaag aaaagcataa actaaagaaa gaaacaggcg ctgtgtcatt
     4441 gttttcatcg aatgacctca ttgttctgtc gccgaaaaat gaacattaaa ataagcagaa
     4501 cggaagttaa tagcacactg gtggcggctc cctgaggcca atcgctcagg acaaggaatt
     4561 gattttggat taaatttccc aataaaatgg aacgagcgcc acctaataca ttaggaatgt
     4621 aaaataaagt cattgctggt aacaagacca gcaaacaacc tgataagact ccaggggctg
     4681 tgttaggcaa aaatactcgt gtaaatatag accacttgtt tgcccctaaa tctttagctg
     4741 cctcaattaa cctgaaatcg aaccgttcca tattagtaaa aatgggtagg accatgaaag
     4801 ggaaaagatt gtaaactaag ccaataacga ccgcaaaatt ggtataaagc agtgacaagg
     4861 gagcatcaat caagtgtaat ttcagcaata gggcatttat cacgcctttg tatttaagga
     4921 tagcaatcaa agcataagtt cttatcaatg agcttgtcca gaaaggtatg ataatcaaca
     4981 gcaataaaat agattggtgt tttgatttaa tcattagata gctaaagggg tatgcgatga
     5041 gcagacaaag cagggtggtg acacaggcta tcagtaagga tcggaaaagg atttttgcaa
     5101 aaacaggagt aaacaagtca aaataatttt ccaatgtcaa tggcaaggta acaagatgaa
     5161 tactgtcttg tgataaaaag ctggcaacca gcaccatcaa caagggaatg aagccaaaaa
     5221 taattaacca aaaatacaat aaatacagtg cggatgattt agccttcata aggtaataaa
     5281 acctcccagc caggcagcca ttgtacccaa acggtttcat ggagagtata ttccagctta
     5341 tcatcgtctt catcaaaaaa ctccgaagca ttgatgattt tgcctgatga caattccact
     5401 ttgagatcaa ccgtagagcc tttataaatg atatcaacaa tacgaccagg caacattcct
     5461 tctgtttttt ctacttcgga taagctccaa actcgaatat cttctggacg aacgatcaaa
     5521 tgaagccact cattgacttg ataattgcca gtgtttttac aggatagctg gattccttca
     5581 atattcgtta cgatgtcttg atcctttact gtgtgaacct gaatatcaaa tatattcgct
     5641 tcgccaataa acatggcgac atgcaggttg gcaggggttt catagacagc cttaggcgtt
     5701 ccaatttgtt caatgtggcc atgattaaag acgacaatac ggtcagacat tgataaggct
     5761 tcttcctgat catgcgttac aaaaataaac gtcatattca aggttttttg taattgcttc
     5821 aactcggatt gcatggcttt cctaagccta tagtcaaggg aactcaatgg ctcatctaac
     5881 aacagtactt gaggtctgtt gatgattgct cgtgcaatcg caacacgttg ttgttggcct
     5941 ccacttaact gttttacatt tctttcggct aacaattcga gttgtacaag tttcaacgca
     6001 tcaaagactc gctccttgat ttcctggttg gacgtttttt tgcagcgcaa ggcaaatgcg
     6061 acattttcga aaacggataa atgggggaaa agggcataac tttgaaagac cgtgtgtaca
     6121 tctcgttttt gaggtggtaa ctgattaaca cattggccat taatgaaaat ctcaccttgc
     6181 gttggttgtt caaaaccaga tataagccga agaagggttg tttttccaca acctgagggg
     6241 cccagtaagg ttaaaaattc accgtgattg acgtttagcg aaacattatt caaaattggt
     6301 gtattgccgt atgatttata aatttgacgg atttcaatga gcggtgttgt catctgcttt
     6361 attaaataaa aaactccaat tatgtgtgct gtgacttatc ttgtccagtc aatcacaaac
     6421 tttgtgccca atattaaaaa attagtcaac tattgtctat ttttgtgttt tgtacatgag
     6481 caaaaaaatc agtcatgcca gcataggcgg gcaaccatct atcaacataa tccaatgctt
     6541 aggtcaagcc actgaggatt tttttgttcg atcaagttta ttttccattg cctgttccat
     6601 ttcttgatac gtttttctct ggttattgct tcataaacat cggtgtgaat ttcataataa
     6661 actaaccgat gtacgttgta tcttttagta aatccttcgg ccaaaccttt tttgtgctga
     6721 tatattcgtt gcacaagatt actggtaatc ccggtataaa gggtcccata tgccttacta
     6781 gctaatatat aaacaacata ttgtttctct tccattattc atcctggttg gttgctataa
     6841 cctgctgcaa ttttgcgatc gagattatat gatttttatt gtatatcagc gagttacttc
     6901 atcttgttca tatggtaagc caggaatggt tgcccgccta tgctggcatg accgattttt
     6961 tgcttatgca cagagatttc gcggcggata gtatgacttt atccagtaaa tcatttattg
     7021 aattttccaa tcatcaggat tgtcatgaga taaattttta aaatgttcca gtagttcatt
     7081 cattccctca cgccctgttg tatctttttt gttttttgca acaaactgct ttccaaaaag
     7141 attgctttgg taatataaat cgggattctc catatcaatg acttcagcaa atactttggc
     7201 tacttccgct gcattttgaa aaacagtatt ctgatggaac cattgataag cttgttcagt
     7261 aaagttttta tagtatttct caacaatatc agagcgagag ccttcaaatc gggtcaattc
     7321 tgtcgcaatt ggtccaggtt ggaaattaat tactttgata ttccatgggg ccagctcaat
     7381 cgctaaggat tcttgtaatc gttcaaatgc tgcaagtgct gaaaaataga ttgcgacacc
     7441 tggaatacta actggaacag aggtaaaaat cgtggatgtc gttgttaata aacctttttt
     7501 cctttccctc attacaggga tgaatcccct gattaaagac aatgggccaa atactttggt
     7561 ttgaaaggtt gttaatacat cttgagtggt tgtgctttca acagggccaa tagccacatt
     7621 agcagcattg ttaaccaatg cgtcaatgta ttgatattga cttaagactt ctttaataaa
     7681 gcggtcaatt gaattttcat cgcaaagatc caatattttt acatccaaat tgctggagtt
     7741 tgggaggttt gctttgttaa tttggcttgt ttcaagattt ctcaccgttg caatgacttg
     7801 atgcccgcgt tttagcattt cacatacaag attatatccc acgccggatg tttttgaaca
     7861 acctgtgacc accactattt ttttatccgt ctttgtcatg tccctatcct aatgatcttt
     7921 tagagtaata aactgagtat agccctttta tttattcaga acttatgaaa accaccaatc
     7981 tctttgttaa aaaagttttt aattcggtca tttattatcg attttccaaa ctcctaactg
     8041 attttttgaa agcagaaaaa tattggttga gaaaataaag gatattcagt gatatatagt
     8101 ttattaccga gattaattgc tctcgtttga ttattgggac attctgataa agattgacaa
     8161 tattggagac agagaaatta aaaaaatccc gtatttacct caggtaaata cgggatgtcc
     8221 cttcaaaaga gggcaggcat attacattgt aaaacggttt aactcttcgt tttcttcttt
     8281 ttcttcattt tctgattcag aagatgaaaa ttctgattct tctttttctt cattcactga
     8341 ttcagaagat gacagttctg gttcttcttt ttcttccgct tctttcgcat ctgtttctgc
     8401 cgccagtata tcatcaagat ttaagttgat attttgcatc aggaattgaa gcgccaatag
     8461 actcattatt tccgggctgc cattcatagt agcaaatact aatgtatcat ctgcctgaat
     8521 atccataata tggaagtgac cgtggccatg agggcatggc atgactagag cattaggagt
     8581 aaaaccaaaa agagtcaata tactgcttct tggttgttga tttccttgct cgtttccttg
     8641 ttgattagtt tgttgattat cttcttcaga acgtctttca gtttttggtg aagcgggggg
     8701 ggttaaaggc ataatcatta c
//
LOCUS       DAXLDT010000123         8708 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXLDT010000123 DAXLDT010000000
VERSION     DAXLDT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.123"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..412)
                     /locus_tag="ACF2SP_002320"
     CDS             complement(<1..412)
                     /locus_tag="ACF2SP_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HFD2360095.1"
                     /translation="MLYFYLKFIHVLSSTILFGTGIGTASVMIYGHRTKNPIVIAAIS
                     KYVVFADWIFTGTSGILQPLTGFAMIYLAGFSWTSLWILGSILGYVVAACCWFPVVCL
                     QIKMRDLAIETVKNNTTLPEKYHRYFLYWFILGWP"
     gene            complement(412..1710)
                     /locus_tag="ACF2SP_002321"
     CDS             complement(412..1710)
                     /locus_tag="ACF2SP_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFD2360096.1"
                     /translation="MKILVTGASGFIASQFVTDLLIAGHEIICCVRNTKHTQRIFPGA
                     QVIFCDFINDTKPEIWSKRLQGIDVVINCVGILYHPDERIIWNVHYETPRALFDACIN
                     SGVKKIIQISALGIDKVDVSYAASKKAIDDYLLTLSIPSVIVRPSYVYGKGSYGGSSL
                     FRGIAGTPFFTAIPGQGTQKFQPISLNDLSEAIVRLVSTPVTETIILHAVSKKIITLE
                     EIIIKLRSWLGLTKTRIIHIPLTFIRLLTRLGDLLPYSTINTVSYKMLASDNITTQEE
                     TQRFADYVNFTPRDYEEGLYSQPSSVQDHWHAQLYFLKLPLRLGISFVWIWTALCCLF
                     FYPKSDSLSLFAQIGFSSLWQLFLFYASALWDGLLGIAMLFNYRFKLTSILQLITILV
                     YTVIITIKLPYLWLDPFAPIAKNIPLLIAILIGLGLESDK"
     gene            1954..2742
                     /gene="truA"
                     /locus_tag="ACF2SP_002322"
     CDS             1954..2742
                     /gene="truA"
                     /locus_tag="ACF2SP_002322"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFD2360097.1"
                     /translation="MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISV
                     VCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARY
                     SAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
                     QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDV
                     LNAKDRKLGAETAPSYGLYLVQVTYPKEFGLLQNNPGPLFLWEK"
     gene            2743..3366
                     /locus_tag="ACF2SP_002323"
     CDS             2743..3366
                     /locus_tag="ACF2SP_002323"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFD2360098.1"
                     /translation="MNPSRIRIKMCGMTRSEDIQYAIDLGVDAIGLIFYPKSARNVSL
                     EKARIIVNNIPPFVDIVAVLVNPEQSFVQLIINEIPVQLLQFHGEESSEFCRQFNKPF
                     IKAIHPKTAIQIQSAVDEFFDASAILLDTPSDKERGGTGLTFDWNIIPENLSKPYILA
                     GGLNESNILEAITMCHPYAVDVCSGIEASPGVKDHLKMSRFIKAIWG"
     gene            3368..4567
                     /gene="trpB"
                     /locus_tag="ACF2SP_002324"
     CDS             3368..4567
                     /gene="trpB"
                     /locus_tag="ACF2SP_002324"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFD2360099.1"
                     /translation="MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQD
                     FLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
                     VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEAR
                     AQHMEKTGRLPDALVACVGGGSNAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASL
                     IAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAIND
                     SEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVA
                     AIDGITI"
     gene            4593..5411
                     /gene="trpA"
                     /locus_tag="ACF2SP_002325"
     CDS             4593..5411
                     /gene="trpA"
                     /locus_tag="ACF2SP_002325"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFD2360100.1"
                     /translation="MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSG
                     ADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGY
                     LNPIEQYGYDLFAQQAVEAGADGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
                     ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPE
                     MAAQVAEFADGVIVGAALINEIIEAYEAKKDPLQASGALLSSMRQAIDNIGSMV"
     gene            5408..7063
                     /locus_tag="ACF2SP_002326"
     CDS             5408..7063
                     /locus_tag="ACF2SP_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFD2360101.1"
                     /translation="MTEQTEKRAHFIRQLIIDDLANGKHQGIVTRFPPEPNGYLHVGH
                     AKSICLNFGLAEEFKGKCFLRFDDTNPIKEEEEYVNAIIDDVRWLGFEWCAMTHSSDY
                     YHELYELAIYLIKKDMAYVDSLSMEEIRAFRGTLQEPGRESPYRNRTIEENLDLFARM
                     KAGEFPDGTHVLRAKIDMKSGNLNMRDPVLYRIRHARHQRTGDEWCIYPMYDYAHPIS
                     DALEKITHSLCTLEFQDHRPLYDWLVDNLPLPAKPVQTEFARLNLSHTVTSKRKLREL
                     VEKKIVTGWDDPRLPTLRGMRKRGYPPAAIRQFCEIIGISRSDSVIDMSLLEECVRAE
                     FNKTAKRALCVMDPLKVVIVNYPENKVEQLQAAFYPQNPESESRSLPFSREIYIEKSD
                     FMENPPNKYFRLSPGAEVRLRHAYVIKCQEVIRNDQGEITELHCTYDENTLGKNPEDR
                     KVKGVIHWVSSSHAYPVTIYQYDRLFTDPNPAREDDYFQFLNHNSLQTLQGFCEPAMA
                     EQAEGDVFQFERLGYYCVNSVAEGCVQAFHRVVDLKDTWGKVS"
     gene            7073..8443
                     /gene="cysS"
                     /locus_tag="ACF2SP_002327"
     CDS             7073..8443
                     /gene="cysS"
                     /locus_tag="ACF2SP_002327"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFD2360102.1"
                     /translation="MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSM
                     VAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASERGVSIDELTAQYIDAMNNDTHA
                     LNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSDNGDVCYEVDTFPEYGKLSHKDI
                     EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELG
                     EQFDIHGGGLDLQFPHHENEIAQSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYT
                     IADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLYQAVKDVPPQTADSK
                     LDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALAHTLKNLAGILGFLQKD
                     PESFLQSGLAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTT
                     WRRIAE"
BASE COUNT         2739 a         1530 c         1863 g         2576 t
ORIGIN      
        1 caggccaacc cagaataaac caataaagaa aatatcggtg atatttctct ggtaaagtcg
       61 tgttattttt tacggtctct atggctaaat ccctcatttt gatttgcagg cagacgacag
      121 gaaaccaaca acatgccgca acgacatatc caagaattga tcccagtatc cataaagaag
      181 tccaagaaaa tccagcaagg taaatcatgg caaaccctgt gagtggttgt aatattccag
      241 atgtaccagt gaatatccaa tcagcaaaaa caacatattt tgaaatggca gcaataacaa
      301 tcggattttt tgttctatgg ccataaatca tcacacttgc tgttcctata cctgtaccaa
      361 aaagtatcgt tgaactcagc acgtggataa actttaaata gaaatagagc attatttatc
      421 agactccaaa cccagaccta ttaaaatagc tattaacaaa ggaatgttct tggctatggg
      481 agcaaatgga tctaaccaaa ggtaaggcag tttgatggta ataatcaccg tatataccaa
      541 aatcgtaatc agttgcaata tactggtcaa cttaaatcga taattaaaca acatggctat
      601 acctaaaagg ccatcccata aagctgaagc ataaaataag aataactgcc ataaggatga
      661 aaaacctatt tgagcaaaca aagataatga gtcactttta gggtaaaaaa acaaacaaca
      721 taaggctgtc caaatccaga caaaggatat tccgagtctt aggggaagtt taagaaagta
      781 taattgcgca tgccaatggt cttggactga actcggttga ctatagagac cttcttcata
      841 gtctcttgga gtaaagttaa cataatcagc aaatcgctgt gtttcttcct gagtagtaat
      901 gttatcactg gcaagcattt tatacgaaac agtatttata gttgaatagg ggagcaaatc
      961 accaagtcgg gtcaatagtc gaataaatgt caatggaata tgtattattc tggttttggt
     1021 caacccaagc caggaacgca gttttattat tatttcttct agcgtgatta tttttttgct
     1081 tacggcatgc aaaataatag tttcggttac aggcgtgctc accaatcgta ctattgcttc
     1141 agataggtca tttagagaga taggttgaaa tttttgtgtc ccttgcccag gtatagcggt
     1201 aaaaaaaggt gttccagcaa tccctctgaa tagagaagac cctccgtaag agccttttcc
     1261 atacacataa gagggtctta ctataactga cgggatagag agtgtcagta agtaatcatc
     1321 tatcgctttt ttactcgcag cataactcac atcaacttta tcaattccca aagcagatat
     1381 ttgaataatt ttttttaccc cagagtttat acacgcatca aaaagtgctc ttggagtttc
     1441 ataatggaca ttccatatga ttctctcatc agggtggtat aaaatcccta cacaattaat
     1501 aaccacatca atcccctgca atctttttga ccagatttca ggttttgtat cattgataaa
     1561 atcacaaaag ataacttgtg ccccaggaaa aatcctttgc gtgtgtttgg tattccgtac
     1621 acaacaaatg atttcatgtc cagcaatcag tagatcggta acaaactgtg aagcgataaa
     1681 cccagatgcg cctgttacca gaattttcat aaggctctct tatttttgat tacaattata
     1741 atagaaatac tataacgaat ttaactccta ctattcaaat ttcctcacca ggattaattt
     1801 taatcaacac cagtttggca tatttaggtt aaatgaaata gtatgagttc atgcaaaatg
     1861 ataaaaccca tacaggcaaa gagttaatac tctaaaaatt atagacaaaa caaagagggt
     1921 gttataaatt gaccctttta tcaggaaaat tgaatgcgta ttgccttagt ggttgaatac
     1981 gatggtagcc aatatcacgg ctggcaggct caaacgggtt tgcatactat tcaacaagct
     2041 gttgaatttg cattatcaaa agttgcagac agttctattt ctgttgtctg cgcaggtaga
     2101 acagatactg gggtccatgc gaccaatcag gtaattcatt ttgattgtga gaaagatcgc
     2161 agcattcgag cctggataca tggtgctaat acctttttac ctaaagacat ctgcgtcaaa
     2221 tggggcaagg aaatgccaga aaattttcat gctcgttatt ctgctgtgag tcggcggtat
     2281 cgctatgtca tttataatgg agctatacgg ccagggttat tacgcggtaa tgtgacatgg
     2341 caatatcggc agttggatca tcgtttgatg caacagggtg gacaatgtct tctgggcgaa
     2401 aatgatttta cttcattccg ttccgtcgaa tgtcaatcta atacaccaat gcgtaatatt
     2461 catcaattgc aagttacaag acatggtgat ttggttgttc ttgatattac agcgaatgcc
     2521 tttttacatc atatggtaag aaatatagct ggtgtattga ttgctgttgg ctcgggaaaa
     2581 caccctgttg gttgggttaa agatgtttta aatgcaaagg atagaaaatt aggcgctgaa
     2641 actgcaccgt catatggcct atatttggtc caggtgactt atcccaaaga atttggattg
     2701 ttgcaaaata atccaggccc tttgtttctt tgggagaaat aattgaatcc atctcgtata
     2761 agaatcaaaa tgtgtggaat gacacgttca gaagatattc aatacgccat tgatttaggg
     2821 gtggacgcga ttggcttaat attttatccc aaaagtgcgc gtaatgtctc actggaaaaa
     2881 gccagaataa tagtcaataa tatacctcca tttgttgata ttgttgcagt tttggtaaac
     2941 ccagaacaat cttttgttca actgattatt aatgaaattc ctgtgcagtt attgcaattt
     3001 catggtgagg aatcttcaga gttttgcaga caatttaata agccgtttat caaagcgatt
     3061 catcctaaga cagcaatcca aatccaaagt gctgttgatg agttttttga tgcgagcgct
     3121 atattgttgg atacgccatc agacaaggaa cgaggaggaa caggattaac ttttgactgg
     3181 aatataattc ctgaaaattt atccaagcct tacatattgg caggtggttt gaatgaatct
     3241 aacattttag aggcaattac catgtgtcat ccttatgcag tggatgtgtg cagtggtata
     3301 gaagcttcac ccggagtaaa agatcatttg aaaatgagcc gatttataaa ggcaatatgg
     3361 ggatagtatg attaaaaaag agcttccaga tgagtttggc cattttggcc catatggcgg
     3421 tatgtttgta gccgatacat tagttcatgc tttaaaacaa ttagagcatg cctataccaa
     3481 atatcgtaat gatcaggatt ttctgtctga gttacacaca gaattaaaag attatgtggg
     3541 gcggcccaat cctctatacc atgccgtaca cttaagtaaa aagataggcg gtgcacaaat
     3601 ttatctcaag cgtgaagatt taaatcatac cggcgctcat aaaatcaata ataccattgg
     3661 acaagcctta ctcgccaaac gcatggggaa aactcgagtg atcgcagaaa ctggcgcagg
     3721 tcaacatggc gttgcaactg ctacagttgc cgctaaattt gggtttcaat gcgttgtgta
     3781 catggggtca gaagacatta agcgtcaatc cagtaatgtt tatagaatga aattgttagg
     3841 tgctgaagtg gtgcctgtca cttcaggatc aaagacgttg aaagatgctt taaatgaagc
     3901 attgagagat tgggtgagtc atgtcgacga cacgttttat attattggta ctgttgcagg
     3961 tccccatcct tatcctcaaa tggtaagaga ttttcaagcg attattggtg ttgaagctcg
     4021 agctcagcat atggaaaaaa caggccgttt gccagatgct ttggtagctt gtgtcggagg
     4081 tggatccaat gcaataggct tgttttatcc ctttttgaat gatcagtctg ttatgattta
     4141 tggggttgaa gcaggaggga aaggcataga aaccggtgag cattcagcat ctctgattgc
     4201 tggaaaacct ggtgtgttac acggtaatag aacctattta ttgtgtgatg agtatggaca
     4261 ggtgaaagac actcattcag tatcagccgg tctggattat cctggagttg ggcctgaaca
     4321 tgcttattta aaagatactg gacgggtcat ttataaagca ataaatgatt ccgaggccct
     4381 ggatgccttt cgcctgttaa ctcatacgga aggaattatt ccagcattag aatctagcca
     4441 cgcggtagcc tatgccatac aattggctaa aacgatgtca aaagaacaga gcatcatagt
     4501 caatctatct ggtcgtggtg ataaagatat gcatacagta gctgctattg acggtataac
     4561 aatttaattt tgataaatta ttcaggtaca acatgaaccg tattgataag actctggaaa
     4621 aattaaaagc caatagaaaa aaaatgttaa gtccttatat tactgcaggc gatccatatc
     4681 ctgagttaac agtaagttta atgcatcaat tggttaaatc aggggcagat gtattggagc
     4741 tgggaatacc tttttcagac cctatggcag aaggtcctgt tatccaaaga gcaatggaac
     4801 gagctttggc gcattcaatt cattgtgatg acgtattgaa tatggttagg caatttcgca
     4861 aaacggatac cgaaacgcct gtgattctta tgggatattt aaatcctata gaacaatatg
     4921 gctatgatct ttttgctcaa caggcagtgg aggctggggc tgatggaaca atattagttg
     4981 atttgcctcc tgaggaagct gatggagtat cgcgagtttg gcaaaaacat ggtttgtaca
     5041 gtatttattt atgttcgcca accacctcag ctgaaagaat gaactttata aatcagcatg
     5101 ccaatggtta tctgtattat gtttcattga agggagtgac tggttctgac gcgcttaagt
     5161 tgccagaatt aaaagctcaa tatctacaac gaaaggcaca atcaaaatta ccgcttatgg
     5221 ttgggtttgg aataaaaact ccggaaatgg ctgcacaagt tgctgaattt gctgatgggg
     5281 tcattgttgg ggcggcactg ataaatgaaa tcattgaagc ctatgaggcc aagaaagatc
     5341 cgctacaagc aagcggggct ctattgagtt ccatgaggca agctatcgat aatattggaa
     5401 gtatggtatg acagaacaaa ctgaaaaaag agctcatttt attagacaac tcattataga
     5461 tgatttggca aatggaaaac atcagggaat cgttactcgt tttcctcctg aaccaaatgg
     5521 atatctccat gttggacatg ccaaatctat ttgtcttaat tttggtttgg cagaagagtt
     5581 taagggaaaa tgctttttac gttttgatga caccaatcca attaaagaag aagaagaata
     5641 tgtaaacgcg atcattgatg atgttcgttg gttgggattt gaatggtgtg ccatgacgca
     5701 ttcttcggat tactatcatg agctgtatga attagccatt tatcttatta agaaagatat
     5761 ggcttatgtc gatagcctga gtatggagga aattcgtgct tttcgcggta ctctacaaga
     5821 accagggcgc gaaagccctt atagaaacag aacaatcgaa gagaatctgg atttattcgc
     5881 tcggatgaaa gctggtgagt ttcctgatgg aactcatgtt ttgagagcaa aaatagatat
     5941 gaaatcaggt aatttaaata tgcgtgatcc ggttttatat cgtatacgtc atgcccggca
     6001 ccagcgtaca ggagatgaat ggtgtattta tcccatgtat gattatgctc atcctatttc
     6061 tgatgccctg gaaaaaataa cacattcttt atgtacgttg gaattccagg atcatagacc
     6121 tttgtatgat tggctggttg ataatttacc tttgcctgcc aaaccagtac aaaccgaatt
     6181 tgccagatta aatttatccc atactgttac gagcaagaga aaattacgtg aattggttga
     6241 aaaaaaaatc gttacaggat gggatgaccc acgattgccc acattacgtg gtatgcgcaa
     6301 aaggggatac ccacctgctg caattcggca attctgtgaa ataattggca tatcaagaag
     6361 tgattcagtg attgatatga gtttacttga ggaatgtgta cgcgctgaat ttaataaaac
     6421 tgcaaaacgg gctctctgtg ttatggatcc cttaaaagtt gttattgtaa attatccaga
     6481 aaataaagta gaacagcttc aagcagcttt ctaccctcaa aatcctgagt cagagagtag
     6541 gagtttaccc ttttcccgtg aaatttatat tgaaaagtct gattttatgg aaaatccgcc
     6601 taataaatat ttccggttat ctcctggcgc tgaagtacgc ttacgtcatg cctatgttat
     6661 aaagtgccag gaagttatcc gtaatgacca aggcgaaata acagaattgc attgcactta
     6721 tgatgaaaat actctcggca aaaatccgga ggatcgcaag gtgaagggtg tcattcattg
     6781 ggtgtcttca tcacatgcct atcctgtcac tatttatcaa tacgacaggt tatttacaga
     6841 tcccaatcct gcacgggagg atgattattt tcagtttctg aaccataact ctttacaaac
     6901 cctccaggga ttttgtgaac cggcaatggc tgagcaggca gaaggagatg tgtttcaatt
     6961 tgagcgttta ggttattatt gcgtcaattc agtggctgag ggctgtgttc aagcctttca
     7021 tcgggtagtg gatttgaagg acacttgggg taaagtgagt tgagaggcag acatgttaca
     7081 tttatataat tctttgacca gaaaaaaaga accatttgtt tccttaaggc ccggaaagat
     7141 aggtatgtat gtttgcggaa ttactgtgta tgaccactgc cacttagggc atgcccgttc
     7201 aatggttgcc tttgatgtaa tggttcgtta tctgcgctca caaggattcg atgtaactta
     7261 tgtacgcaat ataactgaca tagatgataa aatcattgca agagcctcgg agagaggtgt
     7321 atctattgat gaattgaccg cacaatatat tgatgcaatg aataatgata ctcacgcatt
     7381 aaatattttg ccacctgacc atgagccaag ggcaaccggg cacattgaaa ctatcattcg
     7441 cttgattcaa cgtttgcttg aaaaaggtaa tgcgtatgtc agtgacaatg gagatgtgtg
     7501 ctatgaagta gatacctttc ccgaatatgg gaaattatcg cataaagata ttgaaggctt
     7561 ggtttcgggt tctcgcgtag agattgtaaa agagaaacgt tcaccgcttg attttgtgct
     7621 atggaaaaaa gcaaaaccgg gtgaacccag ttggccttca ccgtgggggg aagggcggcc
     7681 tggatggcac attgagtgtt cagctatggc tatgcatgaa ttaggtgaac aatttgatat
     7741 tcatggaggt ggattggatt tgcaattccc acatcatgaa aatgaaattg ctcaaagtga
     7801 agcggctacg ggtaaacctt ttgctaatta ttggctgcat gtcggtatgt tgcaggtcaa
     7861 tggcgaaaaa atggcaaaat caatagggaa cttttacact attgccgatg ttttaaaaga
     7921 gcatcatccg gaagtcatcc gctattttct attaagtagt cattatcgca gtcctttgaa
     7981 ttattcagaa gaaaatttgt taaatgccaa aaaagccctt atcaggcttt atcaagcagt
     8041 taaagatgtt cctccacaaa ctgcagatag taaactggat gagtattggc aagagcaatt
     8101 taatcaggca atgaatgatg atttcaatac tcctgttgca ttatcggttc ttttccaatt
     8161 ggctcatgag gtaaacaaaa gtaattcacc tgcattggct cataccctga aaaaccttgc
     8221 tggtattctt ggatttttac aaaaagatcc cgaatcattc ctgcaatccg gcttggcaga
     8281 ggaagaaaaa ttggttatag aacaactcat tgcagagcgt cttcaggcgc gagcagaacg
     8341 caattgggca aaggcagatc agattagagc tgatttattg agcaaaggca tagagctgga
     8401 agatggtgca acaggaacaa catggcggag gattgctgaa taattgttcc ttctatgcgc
     8461 ttctcttgaa tcagaatgga cttctatctc atacttaata taggatatag gttgcaatta
     8521 ccttttcaga aattttaaaa caggtatatt ggctagatta attgctattt attgaagagg
     8581 ttgttcatta agataaattc gaaattacat ttgaattcgt aatgaatata aaaacatatc
     8641 taaaaaaaaa tgatttaacc ttacgatttg ttggatggtt ctttttaata aactcattca
     8701 tattttgg
//
LOCUS       DAXLDT010000124         8703 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXLDT010000124 DAXLDT010000000
VERSION     DAXLDT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.124"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            258..1481
                     /locus_tag="ACF2SP_002328"
     CDS             258..1481
                     /locus_tag="ACF2SP_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFD2360103.1"
                     /translation="MHQEHPGQTDTPKIDPWLIFVILMLMALMQTTTDQYIPSLPAIT
                     TALNSTEASIQLTISMFMLGLSLSHIFYGPLSDKIGRKPPLMFGVGLSILGSLVCFFA
                     PNVSVLILGRFLQGFGIGCCNSVGRSLVRDLFTDRLLAKIGSYVGVVSIFIMAASPTL
                     GGFIQEMAGWKANFMFLFVFGILVWLLAFCILPETNKHLNPEATKIKVMAKNYFTLLR
                     SKIFVGYTLCACFAFAGMVSYLTIAPFLFQNTMGLSPLEFGRLTFFIAGAICFSGIVN
                     SQLVMRKGISFMVCTGVLFMIVGGLSLLLITILGWSQIYFIMISMTLFSAGAGFTFIN
                     AFAGAFHPFPQMAGTVGALYASMQDLSAALTSGFIAGIKGYGQYSLAMILLILGLSSL
                     VAWYYLGLQDDAISN"
     gene            1491..1718
                     /locus_tag="ACF2SP_002329"
     CDS             1491..1718
                     /locus_tag="ACF2SP_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFD2360104.1"
                     /translation="MKITDEELKKIISKLSGSKADDIQDDTALIEDLHLDSLKIVELL
                     AILSEEYQLEVSEEDAMNFHTYKDLYDFTQA"
     gene            complement(2038..3048)
                     /locus_tag="ACF2SP_002330"
     CDS             complement(2038..3048)
                     /locus_tag="ACF2SP_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HFD2360105.1"
                     /translation="MNFFRCEKPIYIKGPCVALPDRIMSNQDVLNWMDSTQNPAVIGF
                     STGIKNRHWVNEDQACSDLAVQAAEQLFMEKPREKHKVNQVILATISGDYPSPPSSPL
                     VQYRLGLQNAGAFDIGAACAGFVVGLHTSAALAQTNDGSVLLIASEIRSKFLNKNNFA
                     TSVLFGDGAAACCVSQDKEEADFRFIASALFADGEVYDAVSTPAGGSRLPAAVCNDNE
                     QFYITIKESTALFVKAVHGMSDSAKDFLKELNLTISDIQWLVPHQGNKNLVLSVAKQL
                     GFPEEKTIKTVEETGNTSGSSVGIALDRLRSDGKIKSGEKVLLVAAGGGGIAACSLLE
                     VI"
     gene            complement(3045..3797)
                     /locus_tag="ACF2SP_002331"
     CDS             complement(3045..3797)
                     /locus_tag="ACF2SP_002331"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360106.1"
                     /translation="MNILITGGSSDIAQAIAKRRSESGDKIIITCSNDDSLNETLAIY
                     RQQNIDVTGFIYNFSDPQACENDLELILREPLDGIILNAFTRVTQLRKLHALPYHAVR
                     TYLDNNLNGNIWLIHRLLPALLKNKFGRLVLISSLSATAGTSRYPVYCAAKAALEGLF
                     LNLAVDYSANNILSNIVRVGLIKTSRTEQFWKNPAYQEKVMQIIPQGTMGEPTQVAEA
                     IDPLLSKTSFMTGSVVTVSGGLPLMRSEGLLS"
     gene            complement(3794..4027)
                     /locus_tag="ACF2SP_002332"
     CDS             complement(3794..4027)
                     /locus_tag="ACF2SP_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFD2360107.1"
                     /translation="MLSNRIEQVLERIGLTNLPENKQAKLEQGGLDSLMLALLIIELE
                     REFEIKIPVIPLEKEHYESINTIEQYLIELGAK"
     gene            complement(4017..5396)
                     /locus_tag="ACF2SP_002333"
     CDS             complement(4017..5396)
                     /locus_tag="ACF2SP_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HFD2360108.1"
                     /translation="MLFLAPDLPNKVAITQDNRAITFDELTHEVLASSKQFLKLPKTI
                     IVLHARHEIGFIIQLLACLETGRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQE
                     ICENKTIQPHPQTALILFTSGSTGSVKAVQLSSINIKANCHAVIKALEFKKIQDQLLF
                     LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAI
                     AKRYPDSAAKINAIVSAGAPLSTALRTDLKQIFPKAIIYNNYGLTEASPRVLTYSSKD
                     PLFTENYAGYPVGDWKVKLSAEHELLINGTQMMLGYLGEKDSTKIQNGWLSTGDIAEI
                     LPNGLVAIKGRRDSLVNIGGEKVSLSEIEQKICQIDVIKEAIVIPQEDKIYGTRLIVC
                     IEKKSLPSSISEQNLIEKIQTQLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGL
                     DNKEKNNAK"
     gene            complement(5401..7146)
                     /locus_tag="ACF2SP_002334"
     CDS             complement(5401..7146)
                     /locus_tag="ACF2SP_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFD2360109.1"
                     /translation="MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQL
                     DQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLL
                     DKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALDSIELNRSSSWQPT
                     SIKGNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHD
                     MGLIGCILTPIYGGIQAIMMSPFSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRI
                     REEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLV
                     TGGTPGSPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPC
                     DFDQVGEIWVQSNSVAKGYWNQPEETKHAFAGKIKDDERSAIYLRTGDLGFLHENELY
                     VTGRIKDLIIIYGKNHYPQDIEFSLMHSPLHHVLGKCAAFVIQEEHEYKLTVMCEVKN
                     RFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCRKHLLDK
                     TLPIVATWQLNKIEE"
     gene            complement(7158..8219)
                     /locus_tag="ACF2SP_002335"
     CDS             complement(7158..8219)
                     /locus_tag="ACF2SP_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HFD2360110.1"
                     /translation="MNILKPNIQFEITGASRILPASGPITNLEILKNFPRTADKSQGF
                     LEKFAEKIGEEFGFHTRYWCHKPWESLDNSRELSSESLAMQAVDKLITHSKSKDVDAF
                     LLGSTTNKRFTGSQAAAVLGNLKLNAPAYDLKTGCSTSLSTLHFAYAMMALGYQKILV
                     CCSETLSKVIDPENEKTWIGLADGAASLLLEKSKEGSFTIEKSFFSTDGQYVNAFTTQ
                     GVFPPTHEQIDSVGYHLVGDETLMKELAYSRYMQMLDNLLPTEKEKNEITWVIPHQVN
                     RKLIDQVLHESHLNNKTIIWDADTIGNIGGASVLYTLARAVEEKLFDRSGKILLMSVG
                     GGLSYAGQVLNYQKASYSN"
BASE COUNT         2407 a         1662 c         1760 g         2874 t
ORIGIN      
        1 ttcagttgat agacaccgga ttttttataa taggctaccc aagtttcaat tcctatgagg
       61 atcaagaaaa gaggtgaggc aaaaaccatt aaatttatat ccataaatat cctttgaaca
      121 gtgatagacg ctttgcaata ggtattttct attttgcaga cgttgaagcc tatgccttat
      181 tttctaatac ttttgttttg cttgtaaata atgatattgt tatcagcctt gttagaaatc
      241 attctaaagg atatttgatg catcaggaac atccaggcca aaccgatacc cctaaaatag
      301 atccttggtt aatatttgtc atattaatgt tgatggcttt gatgcaaacc acaaccgatc
      361 aatacattcc ctctcttcca gcgattacca cagcattgaa tagtacagaa gcgtctattc
      421 agttaaccat ttctatgttt atgttgggtt taagcctatc tcatatcttt tatggtccct
      481 tgtcagacaa aataggacgt aaacctccgt taatgttcgg tgttgggcta agtatattag
      541 gtagtttggt ttgttttttt gcgcccaacg tcagtgtgtt aatcttgggt cgatttcttc
      601 aggggtttgg aattgggtgt tgcaattcag tggggcgatc tttggtgaga gatttattca
      661 ccgatcgttt gctggctaaa attggctctt atgtcggtgt tgtcagtatt tttattatgg
      721 cagcttcacc cactttagga ggatttattc aagagatggc tgggtggaaa gccaatttta
      781 tgttcttatt tgtttttgga atccttgttt ggcttttagc attttgtatt cttcctgaaa
      841 cgaataaaca cttaaatcct gaagctacaa aaatcaaagt gatggcgaag aattatttta
      901 ccttattacg tagcaagatc tttgtgggat atacactgtg tgcttgcttt gcttttgcgg
      961 gaatggtttc ttatcttacg attgcgccat ttttatttca aaataccatg ggattgagtc
     1021 ccttggaatt tggacgttta acttttttta ttgcaggcgc catctgtttt agcgggattg
     1081 tcaacagtca gctggtcatg cgtaaaggaa tatcctttat ggtttgtacc ggtgttcttt
     1141 ttatgattgt cggtggttta agcctgttat taattacgat actgggttgg tcacaaatct
     1201 attttattat gatttcaatg acattattta gtgctggtgc cggatttaca tttattaatg
     1261 cctttgctgg tgcgtttcat ccttttccgc agatggctgg aacggttggg gctttatatg
     1321 ccagcatgca ggatttaagt gcagcactga ccagtggttt tattgcggga attaaggggt
     1381 atggtcaata ttctttagct atgatattac tgattttagg tttaagttct ttagtggcat
     1441 ggtattatct tggattgcaa gatgacgcta tttcaaatta aggggtatta atgaaaatta
     1501 ccgatgaaga attgaaaaaa attatcagca aattatctgg ctctaaagcc gatgacatac
     1561 aagatgatac ggccctaata gaagatttgc atttggattc tttaaaaatt gttgagcttt
     1621 tggctattct cagtgaagaa tatcaattag aggtcagtga agaagatgcg atgaattttc
     1681 atacttataa agatttatat gattttacgc aagcctgata gttaccggtt attccatttt
     1741 ctgtagtaag gttggtgtat ttcgatcaac gactgtcaag ttaagaaggt ggcgaattct
     1801 aatttaacaa tttgaattta aaataaaaac agataaggtg ctttttgata atttttcgat
     1861 tctcgctttg tcccttgtcc gttcaggctg aagacgtgct tgagcgatgt ctcgaagcca
     1921 tgttggtctt gataaaggct tcgagacggc gtaaacgcct cctcagcctg aacggatagt
     1981 gtaattagtt ttagcttgac gcctatgggt aaaatgcctc aacaaacaat gatcaaatca
     2041 gataacttcc aataaagaac aggctgcaat tcctcctcca cctgccgcca ccaaaagtac
     2101 cttttctcca gattttattt tgccatccga cctcaatcga tccaaagcga tgcctacact
     2161 ggatcctgaa gtatttcctg tttcttcaac agtttttatc gttttttctt caggaaaacc
     2221 taattgtttt gctaccgaca aaaccagatt tttatttccc tgatgaggaa caagccattg
     2281 gatatcagag attgtcagat ttaattcttt tagaaaatct ttggcgctat ctgacattcc
     2341 atgcactgct tttacaaata aagcagtact ttcctttatg gtaatgtaaa attgctcatt
     2401 atcattacaa actgcggcag gcaagcggga acctcctgcc ggagttgaga cggcatcgta
     2461 tacttcacca tcagcaaaca acgcagacgc tataaaacga aagtctgctt cttctttatc
     2521 ttgagaaaca caacatgctg ctgctccatc accgaataaa acactggttg caaaattgtt
     2581 tttatttaaa aatttggagc gaatttcgct ggctatgagt aataccgaac catcattggt
     2641 ctgtgctaaa gcagcacttg tatgcaaacc gacaacaaat ccggcacagg cagctcctat
     2701 atcaaaagct ccggcgtttt gtaggccaag tctgtattgt acgagagggc tgcttggcgg
     2761 ggaggggtaa tcccctgaga tagtggccag gatcacttgg ttcactttat gtttttccct
     2821 gggtttttcc ataaacaatt gttctgctgc ctgtacagcc agatcgctac aagcttgatc
     2881 ctcattcacc caatgccggt ttttaattcc agttgaaaac ccaatcacgg ctggattttg
     2941 ggtactgtcc atccaattga gaacatcttg gttgctcatg attctatcgg gaagcgcgac
     3001 acaagggcct tttatgtata tgggtttttc acaacggaaa aaattcatga caacaatcct
     3061 tctgaacgca ttaaaggcag accaccactg actgttacta ctgatcccgt cataaacgag
     3121 gtttttgaca aaagagggtc aatggcttcc gcaacttgag ttggttcacc catggtgccc
     3181 tgtggaataa tttgcattac tttttcttga taggctgggt ttttccaaaa ttgttctgtt
     3241 ctggaggttt taatcaagcc tacgcgtact atattagaca agatattgtt agcactataa
     3301 tcaacagcca aatttaaaaa taacccttct agagcagcct ttgccgcaca ataaacaggg
     3361 tatcgactgg tgccagctgt tgctgaaaga cttgaaatga ggactagtcg cccgaattta
     3421 ttttttagca aggcaggtaa taaacgatga ataagccaaa tgttgccatt gagattgtta
     3481 tccaggtaag tacgaacagc gtgatagggc aatgcatgta atttacgcag ttgggtgacc
     3541 cgagtgaatg catttaatat gatgccatca agtggttctc ttaaaatcag ttccaaatcg
     3601 ttttcacaag cttgaggatc tgaaaagtta taaatgaaac ctgtcacgtc aatattttgt
     3661 tggcggtata ttgcaagtgt ttcattaaga ctgtcgtcgt tagaacaggt aatgatgatc
     3721 ttatcgcctg attcactgcg tcgtttcgct atggcttggg caatatcaga ggagccgcca
     3781 gtaatcagga tattcatttt gcccccagct caatgaggta ttgttcaata gtattgatcg
     3841 attcgtaatg ctctttctcc agtggaatga cagggatttt aatttcaaat tcacgttcca
     3901 actcaatgat taacaaggcc agcatcaaac tatccaagcc cccttgctca agttttgcct
     3961 gtttgttttc aggaagatta gtaagaccaa ttcgttccag tacttgttca attcgattac
     4021 ttagcattat ttttttcctt attatctaaa ccatcaatat tcaaaaccaa cgcttttcta
     4081 tcaagcttcc catgttgatt tctgggtagg gatgacatga gctgtaattg aatgggcaat
     4141 ttcctgggta gtagctgcgt ttgtattttt tctattaaat tttgttcaga aatggaagaa
     4201 ggcagcgatt ttttttcgat acagacaatt aatcgcgtcc catatatctt gtcttcctga
     4261 gggataacaa tggcctcttt tatcacatca atttggcaaa ttttttgctc gatctcagag
     4321 aggctaactt tttctccccc aatattaaca aggctgtctc gacggccttt tattgcgact
     4381 agcccattgg gtaatatttc tgcaatatct cctgttgata accagccatt ttgtatttta
     4441 gtactatctt tttcaccaag ataacccagc atcatttgag tgccgttaat taataattca
     4501 tgttcagcag aaagtttgac cttccaatcg ccaacaggat aacctgcata attttctgtg
     4561 aataaaggat ccttactgga ataagtcaga actctaggag acgcttcagt taagccgtaa
     4621 ttgttataga tgatagcctt aggaaatatt tgtttcaaat ccgttctgag tgcggtggat
     4681 aaaggagcgc cagcagaaac aatcgcgtta attttggctg cgctatcagg gtatcgtttt
     4741 gctatggcat tgatagctac ccagtgagaa ggtacgccac tccacatttg aggaacggta
     4801 ctttgctcca ttagcatttt aatgtctgta aaatgagtga ttagtcgagt ggtcaggcca
     4861 aacattaaac cagggagcaa ttggcccaga aggccaaaag aataggataa aggtaaaaag
     4921 agcaattgat cttgaatttt tttaaattcc agcgctttta tgaccgcatg gcaattggct
     4981 tttatgttga tacttgatag ttgaactgct tttactgatc ctgtgcttcc tgaggtgaag
     5041 agaataagtg ctgtctgggg atggggctgg atcgttttat tttcgcatat ttcctgcaat
     5101 tccccttctt catttatcat tacggcatta cccaaaagag acagtcgtct ttgcttttct
     5161 tcctctgaaa tggaattggg gaaaagggca atggggcgac cagtttccaa acaggctaat
     5221 agttgaataa taaatccaat ctcatgtctt gcatggagga caatgatggt ttttggaagt
     5281 ttcaggaatt gctttgaact tgctaaaact tcatgtgtta attcatcaaa agttattgct
     5341 cgattgtcct gagtgattgc tactttattt ggaagatctg gcgccaaaaa cagcatgatt
     5401 ttattcctca attttattga gctgccaggt agccactatt ggcagagttt tatctaaaag
     5461 atgttttcgg caaaaattcc tgcgaatttt tccgctggta gtatgtggca ttgctttaag
     5521 aggaatcagg actatagtat gtacctccaa ttggtggttt tcgtaaacaa gctcaaaaat
     5581 ctcattgaat aaattgtctt gagctacgtc atccatgaat cgatttttta cttcacacat
     5641 cacagtcagt ttatattcat gctcttcctg aatcacaaaa gcagcgcatt tgcccaatac
     5701 gtgatggaga ggagaatgca tcaggctgaa ctctatgtcc tgaggataat gatttttacc
     5761 ataaataata attaagtctt taatgcgtcc agtaacgtat aactcatttt catggagaaa
     5821 gcccaagtcc ccggttctta aatagattgc gctacgctca tcgtctttaa ttttccctgc
     5881 gaacgcatgc tttgtttctt cgggttgatt ccaatatcct ttggcgacac tgttactttg
     5941 tacccaaatt tcaccaacct ggtcaaaatc acatgggatc aaggtatcag gatctataat
     6001 tttaactgct tgaataggat taccactgct gactaacttg taactgcctg gactgttatc
     6061 gtcagcaaaa tgtacgcgat gatcttgaaa ttgttctttg gccagagtta atgttttgta
     6121 tggacttcct ggtgttcctc ccgtcactaa caaagtagcc tcagccaagc catagcatgg
     6181 atagaaggct tctttgcgaa atccaaactc tttaaatgcc tgataaaaat gttccatggt
     6241 ttctgctcgt actggctcag caccgttgaa agcggtcacc catgaactta aatcaagccc
     6301 ttctttcttt tcttccctga ttcgtttgac acaataatcg taagcgaagt tggggctgcc
     6361 actaatagtg gctctgtatt tagtaatatg ttttaaccag gaaagcgggt tttgtaaaaa
     6421 tgagaaaggg gacatcatga ttgcctgaat tccaccatag atgggtgtca gaatgcagcc
     6481 aatcaaaccc atatcatgat gtgggggcag ccagctgaaa ataatggttt catcattcat
     6541 atgaaaagag gtaaaaattt tattcagatt atccagtaaa ttatggtggc ttaccatcac
     6601 tcctttaggg tgcatggttg agccggaggt gtattgtaaa aacgcaatgt cattgccctt
     6661 aatggaggtt ggttgccaac tactacttct gtttaactca atgctatcaa gtgcaatagc
     6721 aggaattttc aggaatttgg gatttgtatt taattcgtct gcggtgaatt ttttgatgtg
     6781 atccgcaatc atcagtacta tgaccggttt tgagttggta actatgcgtt gtgccttgtc
     6841 caataatttt tcttgggctg gtgggtaaat gggtacagca atgcagcctg cataaaggca
     6901 gcccaaaaat gcctggataa ggggtaatcc aggtgcaaac aataacaaga ccctatctcc
     6961 aggttttgct ccttctgctt gcaaagttgc cgcaattgct ttggcgtgtt gatccagttg
     7021 ctcataagtc atcgtctctt ccaactcttt gttcagaaaa gtacagcttt tcttgttagg
     7081 gctgtgtaaa gcacgtaacc tgacgacgtc aaccagagac tggcactgca aatattcttt
     7141 tttcactata aaacacctta attgctatat gatgcttttt gatagtttag gacttgtccc
     7201 gcataggata gaccaccacc aacactcatc aggagaattt tccctgatct gtcaaacaat
     7261 ttttcctcaa ctgcccttgc tagtgtgtag agaactgatg cgccacctat attgcctatc
     7321 gtatcagcat cccagattat tgttttgttg tttaaatgac tttcatgaag tacttggtct
     7381 attaattttc gattgacctg atgtgggatt acccatgtta tctcgttttt ttccttttct
     7441 gttggcaata aattatcaag catttgcatg tatctggaat aggctaactc tttcattagg
     7501 gtttcatcac ccaccagatg ataaccaacc gaatcgattt gctcatgagt tggcgggaat
     7561 accccttgag tcgtaaatgc gttgacgtat tgaccatcag tagaaaaaaa actcttttca
     7621 atggtaaaag acccctcctt gcttttttct aaaagtaagg aggcagctcc atcagctaaa
     7681 cctatccagg ttttttcatt ctcagggtca atcactttgg aaagtgtttc agagcaacaa
     7741 accaatattt tttgataccc caaagccatc attgcgtagg caaaatgcaa ggtcgataaa
     7801 gaggtggagc aaccggtttt taaatcgtaa gcgggcgcat ttaatttcag gtttccaagt
     7861 acagcagccg cttgagatcc cgtaaagcgt ttattagttg tcgagcccaa gagaaatgcg
     7921 tcgacatctt ttgacttact gtgggttatc agtttatcta ctgcttgcat agctagcgat
     7981 tcagaagaca gttctcttga attatcgagt gattcccagg gcttgtggca ccagtatcga
     8041 gtatgaaagc caaattcttc gcctattttt tctgcgaatt tttcgagaaa accttgagac
     8101 ttatctgcag ttctcggaaa attctttaaa atttccaggt ttgtgatagg gcctgatgca
     8161 ggcagaatcc ttgatgcgcc agtaatttca aattgtatgt taggttttaa gatattcata
     8221 aagtggcctt atcttgtctg gagcgcataa agcttacaat tattgaaatt acactctttc
     8281 tatcctgcca gatcaagaat ttttatcttt ttcgtataaa tagctctaaa cttatacaat
     8341 taagagtagc ctattttggg aattaagatc tttggcagag cattatagaa tttgcgtctt
     8401 aatgcaaaaa tttaaaatat cagattagac cgtatataga tttctttaaa aacctcaatt
     8461 tttgcagtcg tagataaaaa taccagcgat caagtcatta caggatttgt cttcttagtt
     8521 tggtctgtct aaaagtagtc ttagtgcaaa aagtaaattc gtaagcgcgc tgtttaaaat
     8581 gatagagaat aacccttttg tttggatgat tagggttgat gcatacatcc tagatgtgag
     8641 ataggttcct tatgaaatac agaagtacgc ttattgctag tttatcattg tttatatttc
     8701 tgc
//
LOCUS       DAXLDT010000125         8607 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXLDT010000125 DAXLDT010000000
VERSION     DAXLDT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.125"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1088
                     /locus_tag="ACF2SP_002336"
     CDS             <1..1088
                     /locus_tag="ACF2SP_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2360111.1"
                     /translation="VIKEILTLFKDPKGRIVLIAPPLLQLLIFSFAATMEIKNISIGI
                     LNQDSGKHGYEVIQRFIGSPTFSKVFFIQKQSDIKSIIDNQEAMVVMTIPQDFSRKIE
                     SNSQANIQLILDGRRSNATQIVNGYISSIIENYNQEIQKTHGAIMTPPVMVTRSWFNE
                     NLLYLWFTVPSLVCILSMLISLVVTALSVARERELGTFDQLLVSPLTPYEILIGKTIP
                     AIIIGLAEGLLMWVAAVWLFDVPFYGSPLLMVYILFIFIMSTVGVGLFISSMCKTQQQ
                     AILGVFIYMVPVVTLSGYASPVENMPEWLQDLTWFNPLKFTLIAVIGIFLKGMSALEV
                     FITTWPLLLVGAVTLFIAGWFFKQRME"
     gene            1097..2536
                     /locus_tag="ACF2SP_002337"
     CDS             1097..2536
                     /locus_tag="ACF2SP_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFD2360112.1"
                     /translation="MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKIS
                     SSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAEARIRQARAMKQISLANFYPQIN
                     GSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
                     KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYK
                     AGVSSEVDVSLAEGQLKNTESVLPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSL
                     PKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFFPQFNLSVSNLAFQS
                     TSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQE
                     AQSALVRYSQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAET
                     QEVNSEVSSLTALISLYKALGGGWENFEA"
     gene            complement(2861..4642)
                     /locus_tag="ACF2SP_002338"
     CDS             complement(2861..4642)
                     /locus_tag="ACF2SP_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFD2360113.1"
                     /translation="MKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPS
                     PEFLEFVKESTPLANKLRDKWLYELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLG
                     LYKEAIAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRITLALDKRNVLLAR
                     YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKL
                     WQEKKTNIMLNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFA
                     LKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFL
                     ASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPK
                     EEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPEL
                     VYAIIRQESGFREDVTSSAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINI
                     GIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYL
                     KNVMAFYIVYQYRLNQQPDMNRFLTPL"
     gene            complement(4684..5337)
                     /gene="rpe"
                     /locus_tag="ACF2SP_002339"
     CDS             complement(4684..5337)
                     /gene="rpe"
                     /locus_tag="ACF2SP_002339"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFD2360114.1"
                     /translation="MTFQILPSLLSADMTRLGEEVDAVMKAGADFIHFDVMDNHYVPN
                     LTFGPAFCDALIKKFPNLPIDVHLMTTPVDTLIEAFAKAGAKRISIHPDATIHLDRSL
                     QLINNLGCEAGLVLNPATSIDTLTWCAHNLKFVLVMTVNPGFGGQKLIPEVIPKLSKI
                     RQQYPNLDLCVDGGVTPQNIASLAHAGANQFVAGSAIFNSSNYSETIKRMREQLASI"
     gene            complement(5551..6015)
                     /locus_tag="ACF2SP_002340"
     CDS             complement(5551..6015)
                     /locus_tag="ACF2SP_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFD2360115.1"
                     /translation="MKALIAKTYLLADKIFDFLAPIFILLTRLYLAQVFFLSGLTKIS
                     NWQATLSLFQNEYMVPVMSPTLAAYLGTACELGFPVLITLGLGSRLVLFGLFLFNLFA
                     VFSYQFLWTPDGAVGLTDHMQWGIMILMLLCTGPGKLSIDYLIRRFYLKLDN"
     gene            complement(6012..6791)
                     /locus_tag="ACF2SP_002341"
     CDS             complement(6012..6791)
                     /locus_tag="ACF2SP_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HFD2360116.1"
                     /translation="MRNTLLETIQKEWIAQFKEEALSSPLKNRLARSNFDGISVYMES
                     HHATLFNHLKSTFPLCMRLVGENFWYALLHRYINNCESTQYDINQYGDSLPQFIANFE
                     PAKELPYLQQLCAIEWHWHTLKLLSPVNPFDFEAFSSIDEISFQSLCFHLTPNLKILC
                     SEYPIYQIWAMNYFQQDKQIQISGCPEKVVIYQHNDLPCIMLISEEEYIFLELLSSGN
                     QTPWQKVVTELSKWISEEKIGDTLTKALRYQWINHFSLEAI"
     gene            complement(6784..7653)
                     /locus_tag="ACF2SP_002342"
     CDS             complement(6784..7653)
                     /locus_tag="ACF2SP_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFD2360117.1"
                     /translation="MYSNHSTDQAAPYSGCSGIGLRLEHIDNILKEQPTVDYFEVLAD
                     NYMKQSGAQFKQLLKIAEFYPMSLHSVGLSIATSSEPDYQYLQQIKNLAHCLNSKLIS
                     DHLCWTHANQFFTHELIPFPYTEETLSFIIEKTNRIQEYLNQPIMYENVSRYVTYRQN
                     TLSEAEFLNELSTATGCGILLDINNLYVNWYNHGDDPDKYLHSINTKNVWQMHLGGFS
                     KQEGYLLDSHSDKVYQDVWQLYEKAQNLFINTPTVIEWDNDLPDFSLLYDEMCKAKTI
                     IKRVAERSMNHYA"
     gene            complement(7659..7913)
                     /locus_tag="ACF2SP_002343"
     CDS             complement(7659..7913)
                     /locus_tag="ACF2SP_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HFD2360118.1"
                     /translation="MNNISKITMAAVMAAGLSIATIPANADTPNDMEKCQGVAKAGKN
                     DCGTAQHACAGQSKTDGSTTDWIYLPKGTCEKLVNGTVKS"
     gene            8250..>8607
                     /locus_tag="ACF2SP_002344"
     CDS             8250..>8607
                     /locus_tag="ACF2SP_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2360119.1"
                     /translation="MAKDTKMINLALQGGGAHGALAWGIIDRLLEDGRINFDAISATS
                     AGAMNAAVLAYGFATGGKEGAREALHQFWKMVSDAGQIYNPLKKTPLEELLGIGIENS
                     MSFFMFDLMTKILSPYQ"
BASE COUNT         2531 a         1561 c         1729 g         2786 t
ORIGIN      
        1 ttgtcattaa agaaatattg actttattca aagatcccaa gggacgaatt gtattgatag
       61 ctcctccact cctccagcta ttgatttttt cctttgcagc tactatggaa attaaaaata
      121 tttctatagg cattttgaac caggattccg gaaagcatgg ttatgaagtg attcaacgct
      181 ttataggctc accaactttt tcaaaagttt tttttattca aaagcaatct gatatcaaat
      241 ctatcattga taaccaggaa gcaatggttg taatgaccat cccacaagat ttttctcgta
      301 aaatcgaatc gaattcacaa gccaatattc agttaatttt ggatggtcga cgttctaatg
      361 caacccaaat tgtaaatggt tatatcagta gtattattga aaactataat caggaaattc
      421 aaaaaacgca tggtgcaatc atgacacctc ctgtgatggt cactcgaagt tggtttaacg
      481 aaaatttgct ttatctttgg tttaccgttc cttcattggt ttgcatctta tccatgctga
      541 tttctcttgt ggttactgct ttatcggtag ccagagaaag agaactggga acctttgatc
      601 aattattagt atcacctctt actccctatg aaattcttat aggcaagact attcctgcca
      661 ttatcattgg tttggcggaa ggtctcttga tgtgggttgc tgcggtttgg ctatttgatg
      721 ttccttttta tggttctccg ttactgatgg tttatatttt gtttattttt attatgtcaa
      781 cggtgggtgt tggcttattt atttcttcta tgtgtaaaac ccagcagcaa gctattttgg
      841 gtgtatttat ctacatggtg cctgttgtga cgctttccgg ttacgcttct ccggttgaaa
      901 acatgccgga atggcttcag gatttaacgt ggtttaaccc attaaaattt actttaatag
      961 cagtaattgg cattttttta aaaggtatgt cggctttaga ggtttttatc acaacatggc
     1021 cacttctatt ggtaggggct gttacattat ttattgctgg ctggtttttt aagcagcgta
     1081 tggagtaaga aacagtatgg acagaaaatt agcgctactc ttcagtattt cttgtttatt
     1141 tggctgtact gtcggtcctg attatcaacg cccagacatt aaaattcagg agaaatggcc
     1201 aacaagcaaa gtagagaaaa aaatttccag tagtgcccct gttcatttaa cctggtggaa
     1261 ggcatttcat gatccattgc ttgatcgata cattaacgag gcagctacta ataaccttga
     1321 tatcaaagca gcagaagctc gtatccgcca agcccgtgca atgaaacaaa tttcccttgc
     1381 caatttttat ccgcagatca atggatcagc aagtgctaat tccttgcaaa tcagtaaggg
     1441 tacgttggag gggatttttg caaactctcc aacatatact ctaattgata gaaatcaatg
     1501 gatatataat ttgggttttg atgcacaatg ggagttggat ttatttggta aaacaagaag
     1561 aaaagttgaa gcaagcaaag caagcctgga agaagcttat gaatcacgtc gtaccatttt
     1621 actatctacg atagccgaga tcgcaagaag ttatatagaa ttgagaggcg cacaacagtt
     1681 gtatggtatt gcgcaaaaaa atgtggaatt gcaaagaaaa acgttggctc ttgtccgcca
     1741 acgctataaa gcaggtgtta gcagcgaagt cgatgtttct ttggccgaag gccagttaaa
     1801 aaatacagaa tctgttttac ccaattatat tacccaaata caggtaaatg cttatcaaat
     1861 tgccgttatg ctgggtaaag atccgcaaac aattctcaaa gatattgcga aaattcaatc
     1921 tttacccaaa ccaccgcaaa aagtacctat gggattacgt tcggatattc tgcgccagcg
     1981 tccagacata agagcaacag agaggcaatt ggcgtctgcc gtagctgaaa ttggtgtggc
     2041 agttgctgac tttttcccgc aatttaatct ttctgtttcc aatcttgctt ttcagagcac
     2101 ttctgttgat acgttatttc aatccagtag tggattttgg ctcatgggtc ctcaagtcag
     2161 tttacccata tttcatggtg gccagattag agctaatgtt gagagtaaga aggcggttgc
     2221 tcagcaagca gcaatacaat acaaaaaaat aatcctccag gcattgcaag aggctcaaag
     2281 tgctcttgtc cgctatagcc aatcgctcga tacaaaccat agcttgcgcg ctgccattat
     2341 tgctaacaag cgagctgtgc aatttgccaa gaaacgattt caatacggtg aggatgatta
     2401 tttatcacta ctgcaatcag aggctgctct tctgaatgcc gagactcaag aagttaattc
     2461 ggaggtttct tcattgactg cgttgattag tctatataaa gcgcttggcg gaggttggga
     2521 aaattttgag gcttaaacat ccgcgcatgc tctaaaatca ggaattttat caataaatta
     2581 atgtaataag ctatggataa tgtaggtcat tatcttgaat cgttcgggct gaggaggcat
     2641 ttatgccgtc tcgaagcttt gtatggaatt taatgtttct tgccaaggct tcgagacgat
     2701 gcttactcat ctcctaagcc caaacggccc ctggatagcg atagcttctt atataatcct
     2761 cgcccatttc gttaaattag caaaagtcag acaggtagtt cgcaaacgta gatctggcta
     2821 tagaacgatt tacgtttact accatgcatg aagatatctc ttacaaaggt gttagaaacc
     2881 tattcatatc aggctgttgg tttaaacggt actgataaac aatataaaag gccattacat
     2941 tttttagata atttcgtgtt tcttgccagg gaagtgtttc aatccagata tctatttctt
     3001 tgggcgggtg atttttaagc caatagacta cttgtctggg accggcattg taagcggcag
     3061 caacaagaac cgggtggttg ctgaagcgct tggttaagtg ctgtaaataa gcaatcccta
     3121 tattgatatt cttttgagat aaaaacaatt gcttatgatc cgaatacggt attttgctgg
     3181 ttttggaaac agcgctggct gtttgtggca tgacttgcat tagtccttta gccccagcag
     3241 aggatgttac atcttctcta aaaccacttt cctgacgaat aatagcatat acaagctcag
     3301 gcggtatggc gtattttgac gcatatactt ttattgtgtc tttgtaggcc aatggaaatc
     3361 ttaatgctaa ttgattattg agtgtgtcat tattgcttaa gtaaactgat tttccatgcc
     3421 actttagttc ggttgctatc caatagacca gagcacttgc ttcttctttg ggtaattcac
     3481 taatgaagtc atttaataac cttgatgctt gcagtagctg tttggagttg taaaaatttt
     3541 taacttgctc tatgacaggt tgatagggtt tgagaatctc tgtgttggtt gttggttttt
     3601 cattgtcaaa gctgggtctt tttttcaggc gtttgctggc tagaaaacca taataatgtc
     3661 tgtttttagc tagtggctca taaattttaa tcgcctctgc ttttttccct tgttcttcca
     3721 gggagcgagc caaccaatat tgccaacaag gcatctcttt atttggtgag ttgttaatta
     3781 atttttcaac ttgtttccag tttttatttt tcagagcaaa gcggatttgc cagtcgacta
     3841 acacatcggt gtagtattca ggtttgattt tggcaaacca ttcctgagca tcggcatgat
     3901 ttctcatggc cttgtataat gccaaatgtg caagaaaatc ttgtttttgt cgctcattta
     3961 acatgatgtt ggtttttttc tcttgccata atttcactgc tttatccatg ttaatggata
     4021 ctaatctttt taatccataa agatagaaat aatcgttcaa tttgccaggg ttcaacttcg
     4081 aaatattgga aggattctca taaacactgg ttaagttttt tatttctgta tcgtgaggtg
     4141 ttttgtattg ttttaacagg taacgagcga ggagaacatt gcgtttgtct agtgcaagtg
     4201 ttatgcgttg cgtaatgaat ttctgatcaa aattgtcttg ttttagcaat aaagcaaata
     4261 gggtattgca tgcttgtggt ctggattctc ctgttaacca gattggaata gagcctgcaa
     4321 tagcctcctt gtataatcct aaattaaatt tggcaatttg cgcaaagcat ctgaggttta
     4381 tgtcgtttgt gggttgataa tacttattat aatttgccca atcctttatc ctggccagtt
     4441 catatagcca tttatctcgt aatttattag caagcggcgt gctttctttt acaaattcca
     4501 aaaattctgg tgaagggtta ataggcagct gttcatacca tgcgagatag cgattaaatc
     4561 tgtccatgta ggcttgtcca gataaagcaa acaagttgaa agaacagaga agcccacata
     4621 aaaaaattaa taatcttttc ataattcctc ttaataaggc ttgagggcaa ttatttggca
     4681 cttttaaatg cttgctaatt gttcacgcat acgtttaatg gtttcactat aattactgct
     4741 attgaatata gcagaacctg caacaaattg gttagctccg gcatgtgcca atgaagcgat
     4801 attctggggt gttactccac catcaacaca aagatcgaga tttgggtact gttgccgaat
     4861 tttagagagc ttgggtataa cctccgggat aagtttttga ccgccaaatc ctgggttaac
     4921 tgtcatgacc aaaacaaatt ttagattatg ggcacaccag gttaatgtgt cgattgacgt
     4981 agctgggttg agcactaaac ccgcttcaca tccaaggtta ttgattagct gcaaactcct
     5041 gtctaaatga atagtagcat ctggatgaat actgatacgt ttcgcacctg ctttggcaaa
     5101 tgcttcgatt agagtatcaa ctggagtggt catcaaatgg acatcaatcg gcaaatttgg
     5161 aaattttttg attagagcgt cacagaaagc ggggccaaag gtcaggttgg gtacataatg
     5221 gttatccatg acatcaaaat gaataaaatc agcgcctgct ttcataacgg catctacttc
     5281 ctctcctaaa cgggtcatgt ccgctgacag taaagagggt aaaatttgga atgtcatgat
     5341 ttagtaattc aacggtttgc ttttacatat aataagctga ctttggtaaa cattccagca
     5401 ttacgatttg gattcatatc gagttttatt gttttaatcc tggatttgag ttggagcaaa
     5461 tcaagataat aatactccat cctctctgat tcgttagaga ggatggtata gaatgcttga
     5521 tgtttcgaac tatggagttt aaagattcaa ttaattgtct aatttcaaat aaaatcgtct
     5581 gattaaataa tcaattgata atttccctgg cccggtacat aaaagcatta aaatcatgat
     5641 tccccattgc atgtgatcgg taagaccaac ggcaccatcc ggtgtccata ggaattgata
     5701 agagaaaacg gcaaataagt taaatagaaa aagtccaaat aaaacgagtc tggatccaag
     5761 tcctaatgta atcaatacag gaaagcccag ttcacaagct gttcccagat acgcagctaa
     5821 agtaggcgac atgaccggaa ccatgtattc gttttggaat aaagaaagag tagcctgcca
     5881 attactgatt ttggttaagc cagatagaaa aaaaacctgg gcaagataaa ggcgggttaa
     5941 aaggataaaa attggggcaa ggaaatcaaa aattttatca gccagcagat aggtttttgc
     6001 tataagtgct ttcatatagc ctccagtgaa aaatgattaa tccattgata acgaagcgcc
     6061 ttggttagag tgtcaccaat tttttcttcg ctaatccatt tacttaattc tgttaccact
     6121 ttttgccatg gtgtctggtt tccagaggat aagagttcca gaaaaatata ttcctcctca
     6181 ctaattagca taatacaagg taaatcatta tgctgataaa taacaacttt ttcaggacaa
     6241 cctgatattt gtatttgttt atcctgctga aaatagttca tagcccaaat ttggtaaata
     6301 ggatattcag aacagagaat ttttaaattg ggtgtcagat gaaaacataa agactgaaag
     6361 ctaatctcat ctatgctcga aaaagcctca aaatcaaaag ggttaacagg ggaaagaagc
     6421 tttaaggtgt gccagtgcca ctcaatagcg cataactgct gcaagtaagg cagctcttta
     6481 gccggctcaa aattcgcaat aaattgagga aggctatctc cgtactgatt aatgtcatat
     6541 tgagtggatt cgcaattatt gatatagcga tgcaataaag cgtaccagaa gttttccccg
     6601 accaaacgca tacagagagg aaaagttgat ttcaaatgat taaataaagt agcatggtgg
     6661 ctttccatgt agacactaat accatcaaaa ttggatcgag cgagcctgtt ttttaagggg
     6721 cttgatagtg cctcctcttt gaattgtgct atccattcct tttgaattgt ttcaagcaga
     6781 gtattacgca taatgattca tactcctttc tgccacacgc tttattatgg tttttgcttt
     6841 acacatttcg tcatacagga gtgaaaaatc tggcaagtca ttatcccact cgataactgt
     6901 tggcgtgttt ataaagagat tttgagcctt ttcataaagt tgccaaacgt cttggtatac
     6961 tttatcacta tgactatcca gcaaatatcc ctcttgtttt gaaaatcccc caagatgcat
     7021 ctgccatacg tttttggtat tgatcgaatg aagatactta tccgggtcat caccatgatt
     7081 ataccaattc acgtataaat tattgatatc caataagata ccacaacctg tagcagtact
     7141 taactcattg agaaactcgg cttcagataa tgtattttgt cgataagtga cgtatcttga
     7201 tacgttctcg tacattattg gctggtttag gtattcctga atgcggtttg ttttttcaat
     7261 aatgaatgag agcgtttctt cagtataggg aaacggtatc aattcatggg taaaaaattg
     7321 attggcatgg gtccagcata aatgatctga tattagcttg gagtttaaac aatgagccaa
     7381 attttttatt tgctgcaaat attgataatc aggttcagag gatgtggcga tagataagcc
     7441 aacgctgtgt aaactcattg gataaaactc tgcaatcttg agcaactgtt taaattgagc
     7501 tcccgattgt ttcatgtagt tatcagccag gacttcaaaa taatcgactg tgggctgttc
     7561 ctttaaaata ttgtcgatgt gttcaagccg caatccaatg cctgaacatc cagaataagg
     7621 cgcggcttga tcggtagaat gatttgaata cattctaatt agcttttaac tgtgccgtta
     7681 accagctttt cacaagtacc tttgggcaga taaatccagt cagttgttga accatctgtt
     7741 ttagattgac cagcacaggc atgttgagct gtaccacaat cattttttcc agctttggcc
     7801 acaccttggc atttttccat atcatttgga gtatctgcat tagcaggtat ggtagctatt
     7861 gaaagtccag cagccatcac ggcagccatt gttattttag aaatattatt catgatacct
     7921 atcctttgtt atatgtaatt aatccatatg ttatttaata aaccccttgc ctaaccgggt
     7981 tttttcattg tattcctgta tgcaaacaca gctcctcaaa atgtttgttt cttgctctaa
     8041 aaaacaatca ggttaggcaa gaggtctaat aaaataacgt agttagtata gtaccatttt
     8101 tcgatttgtg acaggacatc aacttatttc tcaaagatgg aaaaattttt aacatggttt
     8161 gtttttattt caaacaggat agaaaacatt tcttagatct ttgccttata cttaataaat
     8221 cacagcaaaa ggaagaggag cattgtatca tggcaaagga taccaaaatg attaatcttg
     8281 ctttacaagg aggtggtgct catggtgctc ttgcctgggg tattattgac agacttcttg
     8341 aagatggtcg tattaatttt gatgccattt ctgcaacgag tgctggtgct atgaatgctg
     8401 ccgtacttgc ctatggattt gcaaccggcg gaaaggaggg ggctcgagaa gctctgcatc
     8461 aattttggaa aatggtgagc gatgccgggc agatatacaa cccacttaaa aaaactcctt
     8521 tggaagaact attgggcata gggattgaaa attcaatgtc cttttttatg tttgatttga
     8581 tgaccaaaat tctttctccc tatcaat
//
LOCUS       DAXLDT010000126         8602 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXLDT010000126 DAXLDT010000000
VERSION     DAXLDT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.126"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2610
                     /gene="legK7"
                     /locus_tag="ACF2SP_002345"
     CDS             <1..2610
                     /gene="legK7"
                     /locus_tag="ACF2SP_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector kinase LegK7"
                     /protein_id="HFD2360120.1"
                     /translation="GWLNELAKFSINPNASFFLKGMQFAKAITEEIKNKPEINSSEVN
                     IYQLMQERDQLLKEVEFEKCATRYAEINFLLNELALNDKKTKEIVERQTEILRLVAPK
                     IKAIKGESIDNLPVIPSYKTKELGNHVNNFNFKFTMSGWEAPFVFRVEDRHELGKEQE
                     LHSYGVSKYFIEDYSVFMMRFKAEDGSTVYKPVILSQFANQNNLEEIAKQLKDGSPKN
                     IAPRIGYYFVQLTDFCLKLIETHNYHPDIKLNNFLVHNNRVLVSDRKTFTTNDNPLAS
                     EILTSPLFAPDEFLKCLLFNKEGDPVGYNRNALWKRMNMPQFMAYQLGMALKQFLILT
                     QLDELPDDFRNPDHSAVSHFKTPSRQIINLSLLVQELTRLDPDKRMTIKQFQTLLNFK
                     NLPPDAFYQKVEEVFPSSQLGIAEDIEALNKVLNSDLKGEALLKQANPVFTKLSKYDP
                     KETRLTRLAEKLAIRCFSNVSKPYFQKLSGLIESALEKDVNEIRQLLNPQSNSEGLLQ
                     KASPIFMKLFERQPEVPQLTDLAKQLAAQCFDESSKNYFTRQLPLLIEEELLNQDWEQ
                     APWYRKALHWLTFGYLRVDNVTEISSLQIPEHTKGKEFQIYFSQLLFLPSEEIKNMFT
                     SKDSQTAEFLLGYIEKEREKRETETPSVQIPVDLKGEEFQMHFPQLEFLPSKDFESIG
                     EKEGEHLECFIFANLQEILSHNNSDSSERNSTSEPSDNESEIHLNAGNVDTIIIANEP
                     NPTEAKPQKKDQDGAKQNNPAKASSPEGKVSTPELDEHPKKQITDDVPKPKTKRSVHF
                     FDEAKKPKPKEKENASPAVDETPPAKRKNVCRIDSVRSTLFRGDGSHRQKIKEQRPRL
                     SEIAWEPPKPNLQ"
     gene            2914..5559
                     /locus_tag="ACF2SP_002346"
     CDS             2914..5559
                     /locus_tag="ACF2SP_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360121.1"
                     /translation="MDVGQKIRILKRYLKEKSVTPEDVNLNDQDLTAYAGLDKLLDYI
                     SIHNNGLFDFTTLESDEKQWKQYVDILNYSATYSPGSPPALIDTSERKAQFGLQYWLN
                     TRTIEALPRDNYYTRTVTLSDEADEEFDQLITESRAKLYKIAQTFPEISSFNPANIIQ
                     VTNQISSIKEKLDTLTGNNPQITLLQKKLNVLTDLNKINQLERLNIPQDNGSNLSYCT
                     LAETIEGVTEKIPLITLAKVKEQLRHLYSQNELDLDSYSESREQYYKLGIEGILKLPK
                     HGDIKEVQREAIALNISRLMGLDTTSSTTVSYNGHPALFIPFDNINLLNEFSSGKIFK
                     VGMGLSGQSYTHYSTIKPLGEGIQADLYIKDFGHALALLYLCCDTDAIGGYCKNKALR
                     NYSSLYIFDQVITDTDKFILDSRISLQPDQFIMKHTRHGQGRNRTLIEDSSLVTKYAS
                     LMRLKELKRIITQYCDHISWLHHNRTQAIKKQLQEELAKDERARLTEELNDVEILEKD
                     AEQIKSQILIRINKITEVLPQTTGTVGSDAIRQCLILEKLLHNPVLYSDDGRPYKNPW
                     TTRQANTVQTINDLQNGKIQIIFKFNIPEEMVDFIKRHGGGDSLTISSPKVITISKAH
                     LDGLRENMLHPEHSPELSLNTNYLDDKDLCIIKMAYRIGNRTKILNAIAEYRVLMNNE
                     QVSSHKKIECLAQTEVKIKALIELANDKGLGMHILKKFYFDAQQRLQKLINPEVLPHD
                     IGQAFAAALRLDRVSELNKVVREAIVQNKVTDPQFVTFLTNCIKMETLATNYKDAQKA
                     SRALLIESQRIINHLKSPVVPLIVQLGGQVHSQAELDTDGLANLENDLQLDFEKPDTE
                     SMITMDLHQAVTKEEMIGNGMTVKV"
     gene            5635..6192
                     /locus_tag="ACF2SP_002347"
     CDS             5635..6192
                     /locus_tag="ACF2SP_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFD2360122.1"
                     /translation="MPVHERRQHFRIDDHIYFDYRILAPGEFCSDIEITNQLLGENGQ
                     KYMEAAQYFQGIDYELAELTQVMAVKEPALAHYLNLLNAKIDYLSRQMLMSSKVQLRK
                     VNISLGGMAFKTMEQIKEKTNLKIILYTKPKMIPIIVDAVVVYSQYQSETHYRTAVAF
                     CNLTSEQEQLLSQHILQAQIKSRAD"
     gene            6268..6537
                     /locus_tag="ACF2SP_002348"
     CDS             6268..6537
                     /locus_tag="ACF2SP_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFD2360123.1"
                     /translation="MSRTVFCCKLKQEAEGLEKQPFPGELGKKVFNEVSKQAWNMWLS
                     HQTMLINEYRLNLIEARAREFLKEEMQKYFFGEGSEKPSGYKEIK"
     gene            6549..7475
                     /locus_tag="ACF2SP_002349"
     CDS             6549..7475
                     /locus_tag="ACF2SP_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFD2360124.1"
                     /translation="MFHDLLEFNEEVLDAINDKNPIVALESTIISHGMPYPDNLTTAI
                     EVENIIRRQGAIPATIAMHQGKIRIGLTQEVMEHLALQKEVIKASRRDISFVLSRKVT
                     ASTTVAATMFCAHMARLPLFVTGGIGGVHQDVTMSFDISADLIELSNTPVTVVCSGAK
                     SILDLPKTLEVLETFGVPVIGYATDEFPAFYSRSSGIPVPQRLNSVEEVANLMSIQQK
                     LNMKNGIVVANPIPVSAELSDEEISPYIKQAHEEAKHMSGKSLTPFLLKRIAELTAGK
                     SLEANIELIKNNAFLGAEIAIAYQKKLFSKKT"
     gene            7526..7601
                     /locus_tag="ACF2SP_002350"
     tRNA            7526..7601
                     /locus_tag="ACF2SP_002350"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7559..7561,aa:Phe,seq:gaa)
     gene            7690..8415
                     /locus_tag="ACF2SP_002351"
     CDS             7690..8415
                     /locus_tag="ACF2SP_002351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360125.1"
                     /translation="MNRNQLVLLNSLIAQQNFSSLQAKVQTLTAEGSTRLYGTSFDTL
                     KSLAMSAHANNVLLFTDGEDSARDSQEMRQLSELVSGLLLEAKARNKFFVIFCGTDGD
                     ALTKDIAKTFDSPIIHTQSADFMSAIKDPEKLKSYAAERGLFTVKYSVHLETGPKTET
                     NTHLIADLSGQIVALDPVEINPNSTLSITIAQGDQTLVDSKKSFTPPVVQQGTNPNGF
                     LNRSSAHSSTYQDPLNTSQLNMA"
     gene            complement(8401..>8602)
                     /locus_tag="ACF2SP_002352"
     CDS             complement(8401..>8602)
                     /locus_tag="ACF2SP_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360126.1"
                     /translation="INYGNRNIITDFIQKKITVFIIFTIGGVRIILVTTLATPRLQVI
                     NRCSLDAVKRNQGFHCTALCHI"
BASE COUNT         3025 a         1659 c         1585 g         2333 t
ORIGIN      
        1 ggctggttaa atgagctggc taaattttca attaatccca atgcatcctt ttttttaaaa
       61 ggaatgcagt ttgccaaagc cattactgaa gaaataaaga ataagcctga aattaacagt
      121 tctgaagtaa acatctacca gctcatgcaa gagcgtgacc aactgttaaa agaagtagaa
      181 tttgaaaaat gtgctactcg ctatgctgaa attaattttc tcctgaatga attagctctt
      241 aacgataaaa aaacaaaaga aattgtagaa agacaaacag aaatccttag gcttgtcgct
      301 cccaaaataa aagccattaa gggagaatcc atagacaatt tgccagttat accttcttat
      361 aaaaccaaag aacttggcaa tcatgtgaat aattttaact tcaaattcac aatgagcgga
      421 tgggaggctc cttttgtatt tcgtgtagaa gacagacatg agttaggaaa ggaacaagaa
      481 ctacactcct atggagtctc aaagtatttt attgaagatt actctgtttt tatgatgaga
      541 tttaaagcgg aagatggttc tactgtttac aaaccagtta tcctcagcca attcgccaat
      601 caaaataatc tggaagaaat tgccaaacag ttaaaagatg gcagtccaaa aaatattgca
      661 ccgcgaattg ggtattactt tgtccagtta accgattttt gtctaaagtt aatagagact
      721 cataactatc atcctgacat taagttaaat aattttctgg ttcacaataa cagagtactg
      781 gtcagcgata gaaaaacatt caccacgaat gacaatcctt tagctagtga aattctcacc
      841 agtcctttat ttgctcctga tgaattttta aaatgtctac tatttaacaa agaaggcgac
      901 cctgtaggct ataatcgcaa tgctctgtgg aaacgcatga acatgcccca attcatggcc
      961 tatcaactgg gaatggcttt aaaacaattc ctgattttaa ctcaattgga tgaattacca
     1021 gatgatttta gaaatccgga tcacagcgcc gtatcacatt tcaaaacacc atccagacaa
     1081 atcatcaatc tttcccttct ggttcaggaa ttaacacgcc tcgatcccga taagcgaatg
     1141 accattaaac aattccagac cttgcttaac ttcaaaaatt taccgcctga tgccttttac
     1201 cagaaagtgg aagaagtttt cccttcctcc caattaggta ttgcagaaga tattgaggct
     1261 cttaacaaag tattgaacag cgatttaaaa ggtgaagcgc ttctgaaaca agctaaccct
     1321 gttttcacta aactatcgaa atatgatccc aaagagaccc gattaactcg cctagctgaa
     1381 aaattggcaa ttcgatgttt tagcaacgtg tcaaaacctt attttcagaa actgtctggt
     1441 ttgattgaat cagccttaga aaaagatgtt aatgaaatca ggcagctatt aaatcctcaa
     1501 tcaaatagcg aggggctttt acagaaagcc agtcccattt ttatgaaact ctttgaaaga
     1561 cagcctgagg taccccaatt aacagacttg gcgaaacaat tagcggctca atgtttcgat
     1621 gaatcttcaa aaaactattt tactcgtcaa ctgcctttat tgattgaaga ggaattgtta
     1681 aaccaggatt gggaacaagc tccctggtat agaaaagcac tgcattggtt aacctttgga
     1741 tacttgagag tcgataacgt aacagaaata tcatccttgc aaatacccga gcatacaaaa
     1801 ggtaaagaat ttcagattta tttttcgcaa ctcttatttt taccctctga agaaatcaaa
     1861 aatatgttta cctccaaaga cagccaaaca gctgaatttc ttctaggtta tatcgaaaag
     1921 gaaagagaaa aaagagaaac ggaaactcct tcagtccaaa ttcctgttga tttaaaagga
     1981 gaagaatttc agatgcactt cccgcagctt gaatttttac cttccaaaga tttcgaaagc
     2041 attggtgaaa aagagggtga acatctggag tgttttatct ttgccaatct tcaagaaata
     2101 ctctcgcata ataacagcga ttcatcagaa agaaatagta ctagcgaacc ctctgataac
     2161 gaaagtgaaa tccatttaaa tgcgggtaat gtagatacaa ttataattgc aaacgaacca
     2221 aatcccaccg aagcgaaacc gcagaaaaaa gaccaggatg gggcaaaaca aaataatcca
     2281 gccaaggcca gtagtcctga aggaaaagta tcaactccag agcttgatga gcatcctaaa
     2341 aagcagataa ccgatgatgt ccccaagcca aaaacaaagc gttctgtaca cttctttgat
     2401 gaggctaaaa aaccaaaacc caaggagaaa gaaaatgcct ctcctgctgt tgacgaaacg
     2461 cctcccgcga agagaaaaaa tgtctgcagg atagatagcg tcaggtccac ccttttcaga
     2521 ggagatggca gccataggca gaaaatcaaa gaacaaagac ccaggttatc tgaaatagct
     2581 tgggagccgc caaaacctaa tctacaatag ccaacagagg ctttctgcag ccaccctgat
     2641 tatacagggt ggctaactta acaaatgtca tggtgggttt gaaactttat tttctctttt
     2701 tgttaaaaaa attgcacatg tttttaacaa cagcataaaa ctatgtctat atttatagaa
     2761 aagaatatac aaaaatcaga gtcaaaataa tgacttattt ttgactggga agttaagtaa
     2821 aaataactaa aaatttgaag gaaaacattg aataggaaaa gcatttttat aaacatggaa
     2881 ataagtctaa ttgatactat aaggaaatag acaatggacg ttggccaaaa gataaggatt
     2941 ttaaaacgtt atttaaaaga aaaaagtgtc acgccagagg acgtcaatct caatgatcaa
     3001 gatctaacag cctatgccgg gctggataag ctattggatt atatttctat acataacaat
     3061 ggattgtttg acttcacaac attggaatct gatgaaaagc aatggaaaca atatgtggat
     3121 atacttaact attccgcaac ctactcacca ggctctcccc ctgctttaat cgataccagt
     3181 gaacgcaaag ctcaatttgg tttgcaatat tggctaaata cgagaacgat tgaagcactg
     3241 ccaagagaca actattatac tcgtacagtc accttatccg atgaagcaga tgaagaattt
     3301 gatcagctga ttacagaaag cagagccaaa ttgtataaga tagcgcaaac ttttccagaa
     3361 atcagctctt ttaatccagc aaatattatc caggtaacca accaaatctc ttcgattaaa
     3421 gaaaaactgg atactttgac aggcaataat cctcaaataa cccttttaca aaaaaaactc
     3481 aacgtactaa cagatttaaa caaaattaat caactcgaga ggctcaatat tccccaggac
     3541 aatggttcta acttgagtta ttgtacgctc gcagagacta ttgaaggagt tactgagaaa
     3601 atacctttaa taacacttgc caaagtgaaa gagcaattga gacatcttta ctctcaaaat
     3661 gagcttgatt tggatagtta tagtgagtcc agagaacaat attataaact tggtatagaa
     3721 ggtatactca aattacccaa gcatggcgac ataaaagaag tacaacgtga agccatagcc
     3781 cttaacatct caagattaat gggtttagac actacttctt caacaacagt aagctataat
     3841 ggtcatcccg ccttgtttat tccttttgac aacatcaatc tattaaatga attttcttca
     3901 ggtaaaattt ttaaagtggg catggggtta agcgggcaaa gttataccca ttactccaca
     3961 attaaaccat taggtgaagg aatccaggcc gatctttata tcaaggattt tggtcatgct
     4021 ctggcattac tttatttatg ttgtgataca gatgctatag gcgggtattg taaaaacaaa
     4081 gcattaagaa actacagtag cctctatatt tttgatcagg tcattacgga cactgacaaa
     4141 tttatccttg attcacgaat cagtcttcaa cccgatcagt tcattatgaa acatacccgt
     4201 catgggcaag ggagaaacag aaccttaatc gaggattctt ccctagttac caaatacgca
     4261 agcctcatgc gacttaaaga attaaaacgc attattaccc aatactgtga tcatatttca
     4321 tggctgcatc ataatcgtac tcaagccatt aaaaagcaat tgcaagaaga actagcaaaa
     4381 gacgaacgcg cacgattaac agaagagtta aatgatgttg aaattttgga aaaagatgct
     4441 gagcaaataa aaagccaaat tctaattcgc atcaataaaa taaccgaagt gctacctcaa
     4501 acaactggaa ccgttggctc tgatgccatc cgacaatgtt taattctgga gaaattacta
     4561 cacaacccgg ttctctactc tgatgatgga agaccttata aaaatccatg gacgacacga
     4621 caagcgaata cagtacaaac gattaatgat ttacaaaatg ggaagataca aatcattttt
     4681 aaattcaata ttccagagga aatggtcgac tttatcaaac gccatggtgg tggagattcc
     4741 ctgactatta gctctcctaa agtgataacg attagcaaag cccatctgga tggccttagg
     4801 gaaaatatgt tgcatcctga gcattcacct gaattgtctc ttaatactaa ttatttagat
     4861 gataaagacc tctgtattat aaaaatggct tatcgtatag gtaatagaac caaaatttta
     4921 aatgctattg cagaataccg tgtccttatg aataatgaac aggtaagctc ccataaaaaa
     4981 atagaatgtc ttgcccaaac tgaagtgaaa atcaaagcac tgatagaatt ggcaaatgac
     5041 aaaggcttgg ggatgcacat tcttaaaaaa ttctattttg atgcgcaaca acggctccaa
     5101 aaattaatta atccagaagt gttgccccat gacattggtc aagcctttgc cgcagctctt
     5161 agactggaca gagtatcaga acttaacaag gttgtgagag aagctattgt ccaaaataaa
     5221 gtgaccgacc ctcaattcgt aacttttttg acaaactgca tcaaaatgga aactcttgcc
     5281 acaaattata aagacgcaca aaaagcaagc agagccttat taatcgagtc tcagcgaatt
     5341 ataaatcatc taaaatcgcc tgtagttcct ttaattgttc aattaggagg gcaagttcat
     5401 tcacaggctg aattagatac ggatgggtta gccaatcttg aaaatgacct acagcttgat
     5461 tttgagaaac cagatacaga atcaatgatc actatggatt tacatcaggc tgttacgaaa
     5521 gaagaaatga ttggaaatgg aatgacagta aaagtttaat agatttcttt taaaactatc
     5581 tgtaatgcag caaatgaaaa gagagatgtt taaacttgga ataatggagt gactatgcca
     5641 gtacatgaaa ggcgtcaaca ttttcgtatt gatgatcata tctattttga ttatcgaatt
     5701 cttgcaccgg gtgaattttg ttccgatata gaaatcacta atcagttact aggtgaaaat
     5761 gggcaaaagt acatggaagc agcgcaatat tttcagggaa ttgattatga gcttgccgag
     5821 ctaactcagg taatggcggt gaaagaacct gctttggcgc attaccttaa tcttttgaat
     5881 gccaaaattg attatttatc tcgacaaatg ttaatgtcga gtaaagttca actacgtaaa
     5941 gtcaacatca gcctgggcgg catggcattt aaaacaatgg agcagattaa agaaaaaacc
     6001 aatctaaaaa ttattctgta tacaaaaccc aaaatgatac ccattattgt tgatgccgta
     6061 gtggtttata gtcaatatca aagtgagact cattaccgaa cagcggtggc tttctgtaat
     6121 ttgaccagtg agcaagagca gttgctttca caacatattt tacaagccca gataaaaagc
     6181 cgtgctgact aagatggaga tgctgtagat tggaactgtt ttttctagga taatgagcct
     6241 tcaaattaaa ataacgaggt tatatccatg agcagaacag tattttgctg caaattaaag
     6301 caagaagcag aaggcttgga aaaacaaccc ttccctggtg aattgggtaa gaaagttttt
     6361 aatgaagtat caaaacaagc atggaatatg tggctttccc atcaaaccat gttaatcaac
     6421 gaataccgcc tgaatttgat tgaagccagg gctcgtgagt ttttgaaaga agaaatgcaa
     6481 aaatattttt ttggcgaagg ttcagaaaaa ccctctggct ataaagaaat aaaataaagg
     6541 attaatttat gtttcatgac ttgttggaat ttaacgaaga agtactggat gctatcaatg
     6601 ataaaaaccc aattgttgct cttgagtcga ccattatctc tcatggaatg ccctaccctg
     6661 ataatttaac tacggcaata gaagttgaaa atattattcg tagacaaggc gccattccag
     6721 ctactatagc gatgcaccaa gggaaaatcc gtattggttt aactcaagaa gttatggagc
     6781 atctcgcgct gcaaaaagaa gtaataaaag cctctcgcag agacatttct tttgtattat
     6841 ctcgtaaagt gactgccagc acaacggtag cagcaaccat gttctgtgcc catatggcta
     6901 ggctccccct atttgtcacc ggaggtatag gtggtgttca tcaagacgtg acaatgagtt
     6961 ttgatatttc agctgatctt atagaactat ccaatacgcc agttaccgta gtctgttctg
     7021 gcgcaaaatc gattctcgac ttaccaaaaa cattggaagt tcttgaaacg tttggggtgc
     7081 cggtcatagg ttatgccaca gacgaattcc ctgcttttta cagcagatcg agtggaatac
     7141 cagtcccaca acgcttaaat tcggtcgagg aagtagcaaa tctcatgtca attcaacaga
     7201 aattgaacat gaaaaacgga atagtcgtcg caaaccccat tccagtatca gcggaactat
     7261 ctgatgagga aatcagtcca tacataaaac aggctcatga agaagccaaa cacatgagcg
     7321 gaaaatcatt aacccccttc ctgttaaaac gtattgctga attaactgca ggcaagagcc
     7381 tggaagctaa tattgagctt atcaaaaaca acgcttttct aggagcagag attgccatag
     7441 cctaccaaaa aaaattattt tcaaaaaaaa cttaatcaac acttgactca aagtcgatct
     7501 cggagtttaa tagcaagcct tgagtggcca gatagctcag tcggtagagc agaggactga
     7561 aaatcctcgt gtcggcagtt cgattctgcc tctggccacc attagaatca atgagttacg
     7621 taaaaatcaa aaaaattcaa attttccatg tagacgcaat gtagacatgt tttcgtaaag
     7681 gctttatgga tgaacaggaa tcaacttgtt ctattaaata gtctaattgc ccaacaaaat
     7741 ttctcatcac tgcaagcaaa agttcaaacg cttacggccg aggggtcaac tcggttatat
     7801 ggaacaagtt ttgatacact aaaatcttta gctatgtcag cccatgccaa caatgtgctg
     7861 ctctttactg atggagaaga cagcgcccgt gatagtcaag agatgagaca gctttcagaa
     7921 ttggttagcg gcttattatt agaagcgaaa gcaagaaaca aatttttcgt tatcttttgt
     7981 ggtaccgatg gagatgcact aaccaaagat attgctaaaa cttttgactc accaatcatt
     8041 catactcaat cagcagattt catgagtgcc attaaagatc ctgaaaagtt aaagagttat
     8101 gcggctgagc gtggtctatt tacagtgaag tactcagtcc atcttgaaac agggccaaaa
     8161 acagagacca acacccatct catagccgat ctatctggtc aaattgtggc attagaccca
     8221 gtagagataa atccaaactc aaccctcagt attaccatag cccagggcga ccaaacactt
     8281 gttgacagca aaaaaagttt tactcctcct gtcgttcagc aaggaacaaa tccaaatggc
     8341 tttttgaacc gttcgtctgc tcattccagt acataccaag atccgctgaa taccagtcaa
     8401 ttaaatatgg cataaagccg tacaatgaaa accttgattc cgtttcactg catcaaggct
     8461 acaccggtta ataacctgta gccttggtgt agccaaagtc gtcaccagga ttatccttac
     8521 cccaccaata gtaaaaataa taaacacagt gattttcttt tgtataaaat ctgttataat
     8581 gtttctgttg ccataattaa tt
//
LOCUS       DAXLDT010000127         8492 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXLDT010000127 DAXLDT010000000
VERSION     DAXLDT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.127"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            228..1508
                     /locus_tag="ACF2SP_002353"
     CDS             228..1508
                     /locus_tag="ACF2SP_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFD2360127.1"
                     /translation="MCAAHHQKNKYIFSTPLIYAAMIGLGIISGLSDLPFLKETGLLI
                     SDLFIKLFKCISLPIISLSIIVTLANYQTDSLMKSVWQRTIKYTFATTLVAALISCFL
                     YILIHPSTVQVNLDAANPKSTSSLGYLGYLSNMIPTNLLSPFLEQQVMGVLLLSIVIG
                     FAVRQIPETEPRETIIRFFRGAHGMFLVMTKWIIKLIPLGLFGFITSTVIQLRSGMDI
                     KGIGQYLVIIVLANLIQGLVILPLWLKSNGIKPFSSMRAMLPALSVAFFSKSSVGTLP
                     VTMNTVEANLKVNPQVSRFVLPLCTSINMNGCAAFIFATVIYLMQSHGIQVSYATMGI
                     WVLVSTIAAIGNAGVPMGCFFLSVSLLSSMNVPITLMGVILPFYGLIDMLETALNVWS
                     DACVTKVVNDKAEVGDGVVGIRPKERSLLNAELG"
     gene            complement(1832..2965)
                     /gene="dapE"
                     /locus_tag="ACF2SP_002354"
     CDS             complement(1832..2965)
                     /gene="dapE"
                     /locus_tag="ACF2SP_002354"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFD2360128.1"
                     /translation="MTDIKQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNN
                     GPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDTDPFSLEEKSGVLYGRGVADMKG
                     SLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
                     VGEPSSSLKAGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSM
                     QWDNGNAFFPPTSMQITYIHCGGHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAF
                     THHNLNPAIEWRLNGEPFLTNKGILLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPY
                     GVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLLID"
     gene            complement(2958..3788)
                     /gene="dapD"
                     /locus_tag="ACF2SP_002355"
     CDS             complement(2958..3788)
                     /gene="dapD"
                     /locus_tag="ACF2SP_002355"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFD2360129.1"
                     /translation="MNSLQDLIEQAFENRQNLSLDTASSDLINAINEVLSGLDNGQFR
                     VAEKINGEWTVHQWLKKAVLLSFKLFPNQIIDAGFCKFYDKIPLKYTDCSNEQFQQSG
                     VRVVPHAMVRRGAYIAKNTVLMPSYVNIGAYIDEGVMVDTWATVGSCAQIGKNVHISG
                     GAGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEKNSVISMGVFLGQSTKIYNRI
                     TGEVSYGRIPAGSVVVAGNLPSHDGSHSLYCAVIVKQVDEKTRAKVSINDLLRANQDD
                     "
     gene            complement(3792..4685)
                     /locus_tag="ACF2SP_002356"
     CDS             complement(3792..4685)
                     /locus_tag="ACF2SP_002356"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFD2360130.1"
                     /translation="MQKNKQNGGQLHAIMAIFALIVSTTLCFIPILFVGLLKLFPNKN
                     WQSLCTRCVDKIATFWCGINNFYVAKAQKIHWEISGLNNLTPKDWYLVVANHQSWLDI
                     VILQRLFNRKIPVLKFFIKDQLKWIPLLGFSWWAMGCPFMKRYSKEYLAKNPHKQGKD
                     IISTRKAIETFKRTPASIMNFVEGTRFTKVKKEQQQSPYNYLLKPKAGGISFIINSMG
                     QQIKSLIDVTIVYAEKNHSLWDFLCKRVKKVKIIIRQLPIPAQFTSANLINDFQAQNA
                     FKEWLNEQWAIKDNLIASLQE"
     gene            complement(4752..5903)
                     /locus_tag="ACF2SP_002357"
     CDS             complement(4752..5903)
                     /locus_tag="ACF2SP_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFD2360131.1"
                     /translation="MNKTHFDTRAIHAGQEPCKSTGAVMTPIYATSTYKQIAPGEHLG
                     YEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAAINTVIDLLGSGDHVVAMDDLYG
                     GTFRLFDKVKTRTSNLSFSFIDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
                     AAIAKKYNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVVSGVVVVGDNS
                     VLSDKIAFLQNSCGAVAGPFDSFLVLRSLKTLSVRMQRHCENANHLANWLSSHPKIEK
                     VIYPGLKSHPQYSLAKEQMNNFGGMISLVLKGSLEDAKRFLARCELFTLAESLGGVES
                     LIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG"
     gene            6052..8367
                     /locus_tag="ACF2SP_002358"
     CDS             6052..8367
                     /locus_tag="ACF2SP_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2360132.1"
                     /translation="MRRQEINYEKVAKAAEKIKKRGIEPSVNEIRDELGLVGNHPQLS
                     ILLEEWYHNQPEFKRKSHTPLTENINLNTDEIREKNVELEKSISLLRATLESTADGIM
                     MVNGHGAVVDWNQKFVEMWRIPSYMMESGKESISFEYILEQLIDPQSLIADVQYLYQN
                     PEWQGELPELHFKDGRIYERFTQPQRVGSQIVGRVYSFRDVTQKRMALDELRIRERAI
                     EASTHGVVIIDVTKNENKVIYVNRAFERITGYGEQHALGKGLLTLLGSNLEEVNHKRI
                     ELAIRESKEETIEMESIKRNGEFYWCEISVAPVKDSFGYVKHYICILNDVTQRRDMED
                     QLLLQATYDSLTNLPNRVLLMDRVEQAILQARKNKAILAFLFLDLDRFKLTNDTLGHS
                     MGDKLLQAIANRLLIVTEDFDTVARLGGDEFVILLTDIDNMLEAETIAQNILKIIEKP
                     IQIDQHSLKITGSLGISFYPRDGDDYESLMKSADLSMYHAKDTGRNNYRVYEPEMNRR
                     VINHMQLDNALRDALKNDELFLVYQPLIDLKQSRVVGFEALMRWHSKILGLVSPADFI
                     PMAEENGMILEMGEWAMKQACIQVKEWHKAGFKNLSIAVNLSGRQFRQKNLPEVVSRV
                     LKSSGLQSRFLELELTESLLIEDIDHVVDTMYALKDMGTKLVIDDFGTGYSSLSYLKQ
                     FPVDKLKIDRSFITEMVSNQNDAAIAKAIINLVHSLNLQVLAEGVENEFQRDFITSHG
                     CDYAQGYFFKAPDTPENILEFLKSLSESKIK"
BASE COUNT         2598 a         1517 c         1678 g         2699 t
ORIGIN      
        1 gccctgtata attgactcag tttatttata ctattgacta gaagaaaaat cgtgttacaa
       61 tatgtactct taatgaacag ttttagctat gggtgatgac ttttatgtca attcaatcat
      121 tatattatta taattcctca ttatttcact ccatatatca agccatctgt tgttgctgct
      181 aatcatactt tgcttaaata attaattaaa gttttaagga ttcagctatg tgtgcagcac
      241 accaccaaaa gaataaatat atttttagta ccccactaat ttatgcagcg atgattggac
      301 ttgggataat aagtggatta tccgatttgc cttttttaaa ggaaacaggg cttttaattt
      361 ctgatttgtt tattaaattg ttcaagtgca ttagtttacc aattatttcc ttgtcgatta
      421 ttgtaactct ggcaaactat caaacggata gtttgatgaa aagtgtatgg caaagaacaa
      481 ttaaatatac ttttgctaca actttagttg cagcattaat cagctgtttt ctatatattt
      541 tgattcatcc aagcactgtt caagtcaact tggatgcggc gaacccaaaa tccaccagca
      601 gtttgggata tcttggctat ttgagtaaca tgataccgac taatttatta tctccattcc
      661 tggagcaaca ggttatgggg gtattgttgc ttagtattgt tatcggtttt gctgttcgtc
      721 aaataccgga gacagagcca cgtgaaacta ttattcgatt tttccgcggt gctcatggga
      781 tgtttttagt gatgaccaaa tggataatta aattaatacc tttggggtta ttcgggttca
      841 ttacttctac tgtaattcag ttgcgatcgg gtatggatat taaagggata ggtcaatact
      901 tggtgattat tgttttagct aatttgatac aaggattagt cattctgcct ttatggctga
      961 aatcaaatgg tattaaacca ttctcttcga tgcgcgcaat gttacctgcg ttatctgttg
     1021 cttttttttc caagtcatct gtcggtactt tgcctgttac aatgaatact gtagaggcta
     1081 atttaaaggt taaccctcaa gtcagccgtt ttgttttacc attatgcaca agcattaata
     1141 tgaatggatg tgccgcattt atttttgcca cagtcattta tcttatgcaa agtcacggca
     1201 tccaggtttc ttatgctact atggggatat gggttttagt atcaactata gcggcaatag
     1261 gtaatgccgg cgttcctatg ggctgctttt tcttaagcgt tagtttactt tccagtatga
     1321 atgtgcccat cacgcttatg ggagttattc ttcccttcta tggtttgatt gatatgttgg
     1381 aaactgcctt aaacgtatgg tctgatgctt gtgttaccaa ggttgttaat gataaagcag
     1441 aagttggtga tggagtggtg ggcattagac caaaagagcg ttcattatta aatgccgagt
     1501 taggttaaag ccagcaaatc ttcaagcgca aatgagtata gcttacatat aagtctccag
     1561 aaaatttttt tgagacttat atctatgaaa tcagtttgta atttataaga gatccgctca
     1621 ctacctggag cggccgtatt tggttcatct tgaacgaaaa taatccttca aaaatgctca
     1681 cataacaaat ctatactgtg ctttttcaag gattattttt atccaaactg aactcaaatc
     1741 cgaccactag aggatattta aagattaggg cactaaacaa ttactgtaat ttggactgtt
     1801 gatgcgaacc ctcattacag aattggcttg attagtcaat taacaatttc tcacagattg
     1861 aaaaatacat ggtttcaagt gtattcaaat cctgtagaga agtgcattca ttaacctgat
     1921 gaatggttgc atttaccagt cccagctcta tgacctcaac cccataaggt gctataaaac
     1981 gcccatctga agttccacca cttgttgaaa gttcggggag agtcccaata tgttcaagca
     2041 ctgtttgttt gcagctttct agcaatatgc ctttattcgt taagaatggt tcgccattta
     2101 atcgccattc tatagcgggg ttaagattat ggtgtgtaaa ggcattaatt actctcgttt
     2161 ttaacgactc gtctgtttgc tctgtcgaat atctaaagtt caaatgcaga tttaattcgc
     2221 ctgggataat attacccgca tgccctccac aatgaatata agttatttgc atggaggtag
     2281 ggggaaaaaa agcattgcca ttatcccatt gcattgaagt gagttcggct aatactgggc
     2341 taattctatg aattggattg tcagccaaat gcggataggc aacatggcct tgctttccgc
     2401 tcagatgtat tttggcactt aatgatccac gccttccaat tttgatgaca tcaccagctt
     2461 tcaagctgct tgaaggttca cctactatgc aataatcaat aacaatccct tgctgctcca
     2521 gtttttgcat cacataaggg gtgcccaaat taaattcatc gccttcttcg ccactggtga
     2581 tcagaaatcc tagtctgcct ggaaaggaag gataagtttt aataaatctt ctggccatat
     2641 gcaacataca ggccaggctg cctttcatat cagcaacccc acgaccatag agcacgccac
     2701 ttttttcttc gagagagaaa gggtctgtat cccattttga tacttcacca acaggaacta
     2761 catcggtatg gcctgcgaac accaataaag gcccaatttt gccatagcag gcaaaaaaat
     2821 tggatacggg cccattattc agttgttgac aagtaaatcc caattgctcc agaaattgaa
     2881 tcatgtattt ttggcagcca gcatcttctg gtgtaataga aggaaaacca atgaggtcag
     2941 tcagaatttg cttaatatca gtcatcttga ttggctctta gtaaatcatt aatgcttacc
     3001 ttcgcgcgag ttttttcatc cacttgtttc acaatgacag cacaatatag actgtggctt
     3061 ccatcatgac ttggcaaatt acctgcaaca actactgagc cagccggaat tctgccgtag
     3121 cttacttcgc cagtgattcg attgtagatt ttagtgcttt ggcctaaaaa tactcccatt
     3181 gatatgacgg agtttttttc tacgataacc ccttccacta tttcagagcg ggcaccaata
     3241 aagcaattgt cctcaatgat ggttgggttc gcttgtaatg gttctaaaac acctcctata
     3301 cctgcgccgc cagatatatg tacatttttt cctatttggg cacaagaacc aacagttgcc
     3361 caggtatcaa ccattactcc ctcatcaata taagcaccta tattgacata agaaggcatt
     3421 aaaacagtat ttttggcaat atatgctcca cgtcgtacca tggcatgagg aacaactctt
     3481 acccctgatt gttgaaattg ctcattacta caatccgtat attttagcgg tattttatcg
     3541 taaaacttgc aaaaacccgc gtctatgatt tgatttggga aaagtttaaa ggataaaaga
     3601 actgcctttt taagccattg atgaactgtc cattcaccat ttattttttc agcgactctg
     3661 aattggccat tatccagacc actgagaact tcattaatgg cattgatcaa atctgaggag
     3721 gctgtgtcga gcgataaatt ttgacggttt tcaaaggctt gttcaattag atcttgaagt
     3781 gagttcatgt tttattcctg taaagaggct attaaattat cttttattgc ccattgttca
     3841 tttaaccatt ctttgaatgc attttgtgcc tgaaaatcgt taataagatt tgccgatgta
     3901 aattgtgcag ggatagggag ttgacgaata atgattttaa ctttcttgac tcgtttacaa
     3961 agaaaatccc agagagaatg gtttttttcc gcataaacga tagtgacgtc aatcaggctt
     4021 tttatctgtt gtcccatcga gttaatgata aaactaatac cgcctgcttt gggtttcaat
     4081 aaataattat aaggggattg ttgctgttct ttttttactt tagtaaaacg ggttccttct
     4141 acaaaattca taatagaagc cggggttctt ttaaaagttt ctatggcttt gcgagttgaa
     4201 attatatctt ttccttgttt atgagggttt ttagccaaat actccttact ataccgcttc
     4261 ataaaggggc aacccatagc ccaccaggaa aagccaagta gaggaatcca ctttaactga
     4321 tctttgataa aaaattttaa aacgggtatt tttctgttaa ataaacgttg aagaatgaca
     4381 atatccagcc aactttgatg attggcaacg actaaatacc aatctttagg agttaaatta
     4441 tttaacccgg agatttccca atgtattttt tgtgctttag ccacataaaa gttatttatt
     4501 ccacaccaaa aagtagcaat cttatcaaca caacgagtgc aaagggattg ccagttttta
     4561 ttaggaaata attttaataa tccaacaaat aatatgggga taaaacaaag agtagtggac
     4621 actatcaatg cgaaaatagc cataatcgca tggagttgcc cgccattttg tttatttttt
     4681 tgcatgaaat acttctccta tacacatctg ccctggttct ttttaacagt cagaaccagg
     4741 atttacatct tttaacctaa agcatgctcc agatcggcac gcagatcatc aatgtgttca
     4801 ataccaactg ataatcttat gaaaccatct tctatgccca gggctttgcg ttgttcgact
     4861 ggaatagagg catgggtcat aattgcagga tgctcaatta aactttctac accccccagg
     4921 ctttcagcca aagtaaataa ttcgcagcga gctaaaaatc gtttggcatc ttcaagacta
     4981 cctttaagta ccaaagatat cataccaccg aaattgttca tttgttcctt ggcaagagaa
     5041 tattgcggat gactttttaa tccgggataa atgacttttt caatcttggg gtggctgcta
     5101 agccagttag ccagatggtt agcgttttca cagtgccttt gcatacgaac ggataatgtt
     5161 tttaagcttc tcagaaccag gaaactgtcg aaagggccag ctacagcgcc gcatgaattt
     5221 tgcaaaaaag caattttatc agacaacact gaattgtcac cgaccacaac gacaccactg
     5281 actacatcag aatgtccgtt cagatatttg gttgctgaat gaagtacaat gtcaaatccg
     5341 agttcaagag ggcgttgaat ccagggagtt gcaaaggtgt tatcggcaac tgtaatcaaa
     5401 ttatattttt ttgcaatagc agcgattttt cgtaaattag ctagctttag catgggattg
     5461 gaaggggttt ccaaccatag taattttgtc ttcggagtta ttgcagcttc aatattctcg
     5521 ggtactgaca tatcaataaa agaaaaggat aaattagaag ttcgggtttt tactttatca
     5581 aaaagccgga aagtccctcc ataaagatca tccatggcaa cgacgtgatc tccggaacct
     5641 aataaatcaa taactgtatt gatggcagcc atacctgaag caaacgcaaa tcctttttgg
     5701 cctgattcaa gacttgctat gcaatcctca taggcctttc tggtggggtt ctgggtgcgt
     5761 gagtattcat agccaagatg ttcgccagga gcaatttgtt tataggtgga ggtagcataa
     5821 ataggggtca tcacggcccc tgtactttta catggttctt gcccagcatg aattgcacgt
     5881 gtatcgaaat gagtcttatt cattttttat tcccataaaa atatttaaat tcaaagaaat
     5941 accaataagc gatcaaaaat ttgattaaaa attatacttt cgtcgctttt attactaaag
     6001 ttcatagtat aaatgccgat atttttatag agtcattcta gagagggatg catgcgtagg
     6061 caagaaatca attatgaaaa agttgccaag gccgcagaaa aaataaaaaa aagaggcata
     6121 gagccgtccg ttaatgaaat tcgtgacgaa cttggtttag ttgggaacca tccgcaatta
     6181 tcaattttgc tggaggagtg gtatcacaat caaccagaat ttaaaagaaa aagtcacaca
     6241 cctttgaccg agaatattaa tttaaatact gatgaaattc gcgaaaaaaa tgtagagctt
     6301 gaaaaatcaa tttcgctttt acgggcaaca ctcgaatcca ccgcagatgg tattatgatg
     6361 gttaatgggc atggagctgt tgtcgattgg aatcaaaagt ttgtagaaat gtggcgaatt
     6421 ccttcttata tgatggagtc gggaaaggaa agtatcagtt ttgaatacat acttgagcaa
     6481 cttattgatc cgcaatcttt gattgctgat gtgcaatatc tttatcaaaa tccagagtgg
     6541 caaggtgaat tacctgagct tcatttcaaa gacggtcgga tttatgagcg ttttactcag
     6601 cctcagcgtg tgggatcaca aatagtgggg cgagtatata gtttccgtga tgttactcaa
     6661 aaaaggatgg ctctcgatga gttgagaata cgtgaacgcg ctattgaggc cagtacacat
     6721 ggtgtggtga ttattgacgt aacaaaaaat gagaataaag tcatttatgt caaccgagct
     6781 ttcgaaagaa tcacgggata tggggagcaa catgctttag ggaaaggctt gcttactttg
     6841 ctgggttcaa atttggagga ggtaaaccat aaacgtattg aattggcaat tagggaaagc
     6901 aaagaagaaa caattgaaat ggaaagcata aaacgaaatg gagaattcta ttggtgtgaa
     6961 atcagcgttg ctcctgttaa agattctttt ggctatgtaa aacattatat ttgcatttta
     7021 aatgatgtga ctcaacgacg tgatatggaa gatcaactgt tgctgcaagc aacttatgat
     7081 tccctgacta atttaccgaa ccgtgtctta ctgatggata gagtggagca ggccattcta
     7141 caagcaagaa agaacaaggc aattttggct tttttatttt tggatttaga tcggtttaaa
     7201 ctgactaatg atactttggg acacagtatg ggggataaat tattacaagc tattgccaat
     7261 cgattactca ttgttacaga ggattttgat actgttgcca gattgggggg cgatgagttt
     7321 gtgatattac ttacagacat agataatatg ctggaagccg aaacaattgc acaaaatatt
     7381 ttaaaaatta tagaaaaacc tattcaaata gatcaacata gtttgaaaat tacaggtagt
     7441 ttgggaatca gcttttatcc ccgagatggt gatgattacg aatctttgat gaaaagtgct
     7501 gatttgtcta tgtatcatgc gaaagatact ggaagaaaca actatcgtgt ctacgaacct
     7561 gaaatgaata ggcgagtcat taatcatatg caattagaca atgcgttaag ggatgcttta
     7621 aaaaatgatg aattatttct ggtttatcaa ccattgattg acttaaagca atccagagta
     7681 gtcggattcg aggctttaat gagatggcat agcaaaatat tgggactggt tagtccggcg
     7741 gattttatac ccatggcaga agaaaatggc atgattttgg aaatgggtga atgggcaatg
     7801 aagcaagctt gcatacaagt aaaagaatgg cataaagctg gctttaaaaa cttaagtatt
     7861 gcggtgaatc tttctggacg gcagtttcga caaaaaaact tgccggaggt tgtatcgcga
     7921 gtattaaaaa gctctggttt gcaatcgaga tttttggaac tcgaattaac tgaaagcttg
     7981 ttgatagaag atattgatca cgttgtagat actatgtatg cattgaaaga catgggaacc
     8041 aagttagtta ttgatgattt tggtacaggt tactcaagct tgtcttattt aaaacaattt
     8101 cccgtagata aactgaaaat tgatcgttcg tttatcactg aaatggtaag caatcagaat
     8161 gacgcggcta ttgccaaagc gattattaac ctggtccaca gtttaaatct tcaggttctt
     8221 gcggagggcg ttgaaaatga atttcaaaga gactttataa catctcatgg atgtgactat
     8281 gctcaaggat atttttttaa ggctcctgat actccagaaa atatattaga atttttaaaa
     8341 agtttatctg aatcgaaaat aaagtaatga ctaattcatt aagttgagaa ttcctgtttt
     8401 ctctcaaaac tcaaattcca tcataaatct caccaaatcg gtatgttttt tgcaagcaaa
     8461 ggttaaatta atcaagtagt ttctgttggc tt
//
LOCUS       DAXLDT010000128         8314 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXLDT010000128 DAXLDT010000000
VERSION     DAXLDT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.128"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..256
                     /locus_tag="ACF2SP_002359"
     CDS             <1..256
                     /locus_tag="ACF2SP_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360133.1"
                     /translation="LANYTLATKKSYDKFGFRQTELPDSIEGDIVLVSMLLTNQRLIQ
                     NYYKKRNFIKKITFIGNLDEKAENHSIGYNHSLACFFISL"
     gene            422..1564
                     /locus_tag="ACF2SP_002360"
     CDS             422..1564
                     /locus_tag="ACF2SP_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360134.1"
                     /translation="MLSKYDKLIYINLAQDQVTANIDTLIIHENPTILVKIPDGSDLK
                     TLVDPDFFPGPITNRTKIYFSAHGHEELDDMVLDRQKENPKIYKLDDVAAYFGKLLTN
                     PDFKNPDITPRLTLVMSVCEGLGFAKNLQKKLFDDYGLLVDVIANKNVIHEQFVKPHH
                     ETTAYLTHRETSIKGVGREHKRPHSKVLLTIDKEGTQQEIDVYELKWIEKVLNTIHQQ
                     VATFGKWADFEKPENIKIQEGLNAFCHDIDALISLLINQDINLTANNLLALLLSCQKT
                     TADPLYKQAMNYEMFQIMTKNLINEGLRYINPKVEIQEYSHALEELQSQKDKMIRDEV
                     APNIFRAKKAIFSALLAEASEYGIDSVLDELEKAKQELSQGKMNPS"
     gene            complement(1722..2954)
                     /locus_tag="ACF2SP_002361"
     CDS             complement(1722..2954)
                     /locus_tag="ACF2SP_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFD2360135.1"
                     /translation="MNRPVVIVDPLSSGIELAPAFKARGIPAIAVTLKPLDWIGFGAN
                     MQTSDFIEIIPDQPNLVEILAKYDPIAIIPGTEEGVPLAEALAINLTPQFANDPEKSQ
                     NRLHKALMQKALQEAGVPALKTLNTASENEVEAWIRTNGLIDSPLIIKPPVSAGSDKV
                     FHIPARGEWKKAFNQVLSEPSKITGKNNETVVVQEQAIGTEFAVGTVSANGKHYLAHL
                     IQYNKTSFNDRKTVYDYVEFVPYSEEIYGELFDYTKKALDALGIRWGAAHNEIMLTKD
                     GPRLIETGARMCGGPVVGFAREATGSSQADKLVEIYVDGGVSTEEYVFKKTVVPVFLK
                     SPAKGKISNVEAFADLSKLPTFLNEHIWFKNGDLVPQTVDYLTSIGIIGLAGDRDSIL
                     LDYEKIRSMESKLVIQTF"
     gene            complement(2951..4099)
                     /locus_tag="ACF2SP_002362"
     CDS             complement(2951..4099)
                     /locus_tag="ACF2SP_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360136.1"
                     /translation="MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGV
                     SLFSLTYLLSAPLFSLIFVNQPVKRMVQLALTVFMFGNLITLLSENIVLFLIGRSLAG
                     AGTGIFTPLCISIAVHFASPSAKGRILSFIWSANSAGVVFGVPAGLYLSSLFHWQLSI
                     ASLIILSLLALIGFSMQNIDIKLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGL
                     YSYVTLIQSGSPNSLSMTLLSWGLGGFIGSSLIGVFIDRTGKPRVIMALILVGLMFAL
                     IAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGIILAAINSSALGLGS
                     ALGTLLGGLLISSGFRGIYLPFSAATLLFFVLIIQLIVINTSHKVNNI"
     gene            complement(4096..5538)
                     /locus_tag="ACF2SP_002363"
     CDS             complement(4096..5538)
                     /locus_tag="ACF2SP_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFD2360137.1"
                     /translation="MSAQVADVVIVGAGPVGLMCAYLGQRCGIHTVIVDKSDGPLEVG
                     RADALNARTLQLLELIDLFDELYPLGKTCNTSSVWADGQFISRQSSWWEELEGCLHKH
                     FLMLGQSYVEKLLDEKLKETAATVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGA
                     DGARSFVRNHFAIPFEIIRPQIIWAVIDGMIDTDFPKVPEIIVFQAETSDVAWIPREG
                     EIDRFYVRMDTKDFTLQEAMDKINHAVRPHSLNFKDIVWFSQFSVKESVAERFFIQDR
                     IFLAGDACHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGASQELLHSYEAERKPVAH
                     GVIETSGELVRSTKYSLNGTHAQDYVRIVQKRAGYITGMGIRYGETGLCGSRLFDFEI
                     FNGIAKTRLYSLLDYMKFTLFIFGDCEVELQPPEFVKVIPIYPTQCQENFWTDNTHYA
                     NQAILVRPDSYIQCAVPLDKMESLFELAQL"
     gene            complement(5535..6371)
                     /locus_tag="ACF2SP_002364"
     CDS             complement(5535..6371)
                     /locus_tag="ACF2SP_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HFD2360138.1"
                     /translation="MNYKVTSLKPFGVLLEPVSEKTKVTDVDIENLRHLFAKNQLVVL
                     RGFCAFQNAEDFSNYCELWGEVSLWPFGKVLELIEQEDPEDHIFDHSYMPLHWDGMYR
                     PQVPEYQIFHCVKAPLPGQGGRTTFSNTILALQFASSEIKELWNKVSGTYQRKMEFYN
                     SKTVSPIITKHPQKDFSVIRYNEPPSADKGHFVNPPDIEFTGIDQEELDFFHRSLNKA
                     LYSADNFYAHEWQNGDIVIADNFSLLHGREGFVSRSPRHIQRVHVLSNPPFDNPGLES
                     YQ"
     gene            complement(6361..7410)
                     /locus_tag="ACF2SP_002365"
     CDS             complement(6361..7410)
                     /locus_tag="ACF2SP_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tyrosine/L-tryptophan isonitrile synthase
                     family protein"
                     /protein_id="HFD2360139.1"
                     /translation="MKNTAFLITENHYPKDYRLKKSPDSDSAMGVAKRILAEVMIFRR
                     VPETISLCSTGCQKCASPHLPKIISAVNENEPVTFILPAFPGKSPNPEKVLGHLPDHA
                     ERLSLNFLGALCQRIKNFYTPGIKIILCSDGRVFSDVVGMNESDVTAYQLELDRLIKE
                     MSLADLSTFNLDYFYKNLHFVQMREELMKSYGQSLDFLKQKIRNGAKPSASPDEQEAN
                     RMYSGITRFLFEDAMHAGQAKSRTAIQKESRVKAYEVIRRSNAWSALIAEHFPKAVRL
                     SIHPQTCGSKKLGIRLIGNESWMTPWHGVAVESNKGYVLLRRSEAEALGAKLICSSDG
                     RRSHYQLMAEVSYEL"
     gene            7521..>8314
                     /locus_tag="ACF2SP_002366"
     CDS             7521..>8314
                     /locus_tag="ACF2SP_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2360140.1"
                     /translation="MSLLLDDIRYFIAASDTLNMTRASEIIGISQPALSYSIKRLENK
                     LGGLLFIRLKNGIQLTKLGEEFKRRSHRLLYEWEQVQNIANPESGFVQGNYTIALHPS
                     VALYTLQYFMPKLQVNYPGLGINFIHGHSREMTEKVISWEADFGIVVNPIQHPDLVII
                     KLSTDEVTVFYANDAQNKLIYDRKLAQSQYILKKISKKITFNGVINSTSLEVVAKLTA
                     LGLGYGILPTRVTYPYKHLKKILNAPVFRDEICLIYRPEKHNNPVS"
BASE COUNT         2651 a         1777 c         1564 g         2322 t
ORIGIN      
        1 attagcaaac tacacacttg ctactaaaaa gtcttacgat aaatttggat ttagacagac
       61 cgaacttcct gactccatcg aaggagatat agtgttagtc tcgatgctat taacaaatca
      121 gcgtctaata cagaattatt ataaaaaacg aaattttatt aaaaaaatta ctttcattgg
      181 taatctcgat gagaaagcag aaaaccattc aattggatac aatcattcat tggcttgttt
      241 tttcatcagc ctctaaatac ctccaacata gagtgaccac ttctaattga ataaccaaaa
      301 tctcaacatg cgatgaagtt attttcttaa attgttctat ttgtttactc catttatggt
      361 gaatttttaa tcaaaaattc tatactttaa gaatatcctc aaaccaaaat ggagctctat
      421 tatgctttcc aaatatgata aactcattta catcaatttg gctcaagatc aagtaacggc
      481 aaatattgat acacttataa ttcatgaaaa ccccacaata ctcgtaaaaa taccggatgg
      541 aagcgacctg aaaaccttgg ttgacccaga tttctttcct ggaccgataa caaatcgcac
      601 caaaatatat ttttcagctc atggacacga agagctagat gacatggttc ttgatagaca
      661 aaaagaaaat ccaaagatat acaaattaga cgatgttgct gcctattttg gcaaattact
      721 aacgaatcca gattttaaaa atcctgatat aacacctcgg ttgacactag tgatgagtgt
      781 atgtgaagga ttaggttttg ccaaaaattt gcaaaaaaaa ttatttgacg actacggatt
      841 acttgtcgat gtgatcgcta ataaaaatgt aatacatgag caatttgtaa aacctcacca
      901 tgaaacaacg gcctatctca ctcataggga gacatccata aaaggagtgg gcagagaaca
      961 caaaagacca catagcaaag tattattgac tattgataag gaggggacac aacaggaaat
     1021 tgatgtttat gaattaaaat ggatagaaaa agttcttaat acgattcatc aacaagtagc
     1081 aacttttggc aaatgggctg attttgaaaa accagaaaat atcaagatac aagaaggcct
     1141 caatgctttt tgtcatgata tcgacgccct catctcgtta ctcatcaatc aagatattaa
     1201 tttgactgct aataacctct tggcattgct gttaagttgt cagaaaacca ccgctgatcc
     1261 attgtataaa caagcaatga attatgaaat gtttcaaatc atgacaaaaa atctcattaa
     1321 tgaagggtta agatatatta atccgaaagt tgagatacaa gagtattcac atgctcttga
     1381 ggaacttcaa tcacaaaagg acaaaatgat tcgagatgag gttgccccta atatatttcg
     1441 ggcaaaaaag gcaatttttt cggcattact tgccgaagct tcagaatatg gcattgattc
     1501 agtcttggat gaacttgaaa aggcaaaaca agaactatct caaggaaaga tgaacccgtc
     1561 ttaattaata ccatgactag tccctgtttc gcttatttat tcttttagtg ggacagcgaa
     1621 aatcaaactc gctaaagctg gtcttagcat aactgcaaaa aaccgttaaa gaccataaaa
     1681 gatagggaaa atatttccat agaagttcag caataaaaac cctaaaatgt ttggataacc
     1741 aatttcgact ccatgcttcg aattttttcg taatccaata aaatggagtc gcggtctcca
     1801 gccagcccaa taattccaat actcgttaaa taatcaactg tttgtggaac aaggtcacca
     1861 tttttaaacc aaatatgttc attaagaaaa gtcggtaatt tcgacagatc ggcgaaagct
     1921 tctacattcg aaattttacc tttagcgggt gatttcaaaa atacaggcac taccgttttt
     1981 ttaaatacat attcttctgt tgaaacaccc ccatcgacat agatttccac taatttatcg
     2041 gcctgactac tgccggtcgc ttctcgagca aagccaacca ctggaccacc acacattcta
     2101 gcacctgttt ctatgaggcg aggtccgtcc ttagtgagca tgatttcatt gtgggcagcc
     2161 ccccagcgaa ttcccaaagc gtccaatgcc tttttcgtat aatcaaataa ttcaccatat
     2221 atttcttcgc tataaggcac aaattccaca tagtcgtata ccgttttgcg atcattaaag
     2281 gatgttttat tgtattggat taaatgtgct aaatagtgtt ttccattagc actcactgta
     2341 cctacagcaa attcagttcc tatagcctgc tcttgcacaa ccacggtttc attgttttta
     2401 ccggtaattt tagatggctc cgataaaacc tggttgaatg cttttttcca ctcccctctg
     2461 gctggaatat gaaagacctt atcactgcca gcagatactg ggggtttaat aataagtgga
     2521 gaatcaatta atccgtttgt cctgatccag gcttcaacct cattttcaga tgccgtattg
     2581 agcgtcttaa gggcaggaac cccagcttcc tgcagcgctt tttgcatcag agccttgtgt
     2641 aatctattct gtgatttttc aggatcattg gcaaattgag gcgtcaagtt tatcgccaac
     2701 gcttccgcga gagggactcc ttcctcagta ccaggaataa tggcaatagg gtcatatttt
     2761 gcaagtatct cgacaagatt tggttggtct ggaataatct cgataaaatc cgaggtttgc
     2821 atgtttgctc caaatccaat ccaatccaga ggtttaagcg tgactgcaat agcaggtatc
     2881 cctcgagcct taaaggcagg agccaactct attcctgatg ataatggatc gacaatgaca
     2941 acaggcctgt tcatatgtta tttaccttat gtgaagtgtt aattactatc agctgaatta
     3001 tcaatacgaa aaacaataaa gtggcagcag aaaaaggaag atagattccc ctgaatccag
     3061 aggaaatcaa taagccgcca agcaacgttc ccaaagctga ccccaagccc aaggccgatg
     3121 aattgatggc ggcaaggata attccctgat tttcatgtaa ttcatataaa atgtgttgct
     3181 ggggagtcac tatagcccat ccgcaagcac cccacataaa aaaagggatt aatcccaggt
     3241 aaggcagatt cctggtgaat ggtatggcaa tcagagcaaa catgaggcca accaaaatta
     3301 aggccataat aacccgtggt ttacccgttc gatcgataaa caccccaatc agtgagcttc
     3361 ctataaatcc tcccagtccc caactcaata gcgtcatact gagcgaatta ggggatcctg
     3421 attgaatcag ggtgacatac gagtatagtc ccaaacttgc caaggcggta aagcaagtaa
     3481 ttccaattac cgatagcgtt tttggctcga ccagaagacg aagcctgcct ccaaatggcg
     3541 aaggtttggg tagttttata tcaatgtttt gcattgaaaa accaatcaat gcaagcaaac
     3601 ttaaaataat aagactggca atcgataact gccaatgaaa caaggaggat aagtaaagtc
     3661 cggcaggaac gccaaacact acacccgcac tgttagcact ccagataaaa cttaaaattc
     3721 gtcctttggc agatgggctg gcaaaatgaa cggcaatgct gatacataac ggcgtaaaaa
     3781 tcccggttcc tgctcccgcc agagatcttc caattaaaaa cagcacgata ttttcagaaa
     3841 gtagcgttat taaattgcca aacatgaaga cagtaagcgc aagctgaacc atacgcttta
     3901 ccggctgatt aacaaaaatc agagaaaaaa gtggcgctga gagaagataa gtcaaactga
     3961 atagactcac tccttgccca gttactgcaa ccggctgccc gagcgtttcg cctatttgag
     4021 gaagcaaacc agcaatcaga aaggtattgc aacctactgt aaacatacct attgctaaaa
     4081 aaatcaggct tttcttcata actgtgccaa ttcaaataag gattccattt tatccagtgg
     4141 cacagcgcat tggatgtagg agtcaggcct tactaaaatg gcctggtttg catagtgtgt
     4201 gttatcggtc caaaaatttt cctggcattg ggttggatat attggaatga ctttgacaaa
     4261 ctccggaggc tgtaactcaa cttcacagtc accaaaaata aataaagtga atttcatata
     4321 atccagaaga gagtaaagcc gtgtcttggc aatgccatta aaaatttcaa aatcaaagag
     4381 tcgagaacca cacaaacctg tttctccata acgaatcccc atccccgtga tataacccgc
     4441 gcgcttttgc acaatcctga catagtcttg agcatgggtt ccattaagcg aatacttggt
     4501 cgagcgcaca agctcaccgg aagtctctat cacaccgtga gctactggtt tacgctcagc
     4561 ttcataactg tgtaaaagtt cttgggaagc gccgaaatgc atgaccatgt gcaatttcca
     4621 catgagatta aacgcatctg caagaccagt gttaaggcct tgcccaccgt ttaccgaatg
     4681 aatatggcaa gcgtcaccgg caagaaaaat gcgatcctga atgaagaaac gctcagcgac
     4741 agattcttta actgaaaatt gtgaaaacca gacaatgtcc ttaaaattta aggaatgggg
     4801 ccgcacggca tgattaattt tgtccattgc ttcctgcaag gtaaagtctt tagtatccat
     4861 ccttacgtaa aacctgtcga tttccccttc cctaggaatc caggccacat ctgaagtttc
     4921 tgcctgaaac acgatgattt caggaacttt tggaaaatcg gtgtcaatca tgccatcgat
     4981 gaccgcccat ataatttgtg gccgtatgat ctcaaaaggt attgcaaagt ggttgcgaac
     5041 aaaggaacga gccccatcgg ctccgatgac gtagcgtgac tgaatacgct ctccattggc
     5101 aagagtcgta agacatcctg cttcattaat ctcaatgttg acaatcgctg ttgaacgttt
     5161 tactgttgcg gcagtttcct tcagcttctc atctaacaat ttttctacat aggattgacc
     5221 aagcataagg aaatgtttat gcaaacaacc ctccaattcc tcccaccatg acgattgtcg
     5281 ggaaatgaat tgtccatctg cccaaacaga actggtattg caggtctttc ctaaaggata
     5341 aagttcatca aataaatcaa ttaattcgag aagttgtaaa gtgcgcgcgt taagggcatc
     5401 cgctctacca acctctaaag ggccatcaga cttatcgaca atcacggtat ggataccaca
     5461 acgttgtccc aaataggcac acatgagccc tactggaccc gcaccgacaa ttacaacgtc
     5521 tgcaacttgt gcgctcattg ataagactcc agacccgggt tatcaaaagg cggattactc
     5581 aaaacatgaa cgcgttggat atggcgaggc gacctggaga caaacccttc tctgccatgt
     5641 aataaagaaa aattatctgc aatgacgata tctccgtttt gccactcatg agcatagaaa
     5701 ttatccgctg aataaagtgc tttatttaaa cttcggtgga aaaaatccaa ctcttcctga
     5761 tcaataccag taaactcaat atcgggtgga tttacaaaat gacctttatc tgcagaggga
     5821 ggttcattgt agcgaatgac tgaaaaatcc ttttgaggat gcttcgtgat aattggtgat
     5881 accgtcttgc tgttataaaa ctccattttc ctttggtagg taccactcac cttattccat
     5941 aattctttga tttctgaaga ggcaaactgt aacgctaaaa ttgtgtttga aaaagtggtc
     6001 cttcctcctt gtccaggcaa aggcgctttc acacaatgaa aaatctgata ttcaggcact
     6061 tggggtcgat acataccatc ccaatgcaag ggcatgtaac tatgatcaaa aatatgatct
     6121 tccggatcct cttgctcaat taactcaagt accttgccaa atggccaaag gctgacttct
     6181 ccccacaact cgcaataatt agaaaaatcc tccgcatttt gaaaggcgca aaagcctctt
     6241 aatacaacga gttgattctt tgcaaataaa tggcgcaagt tctctatatc gacatcagtc
     6301 accttcgttt tttcgctcac aggctccaag agtactccaa atggttttag agaagtcact
     6361 ttataattca tatgacacct ccgccattaa ttgataatga ctgcgccgac catcagagga
     6421 acaaattaat tttgcaccca aagcctcagc ttctgagcgc ctcagcaaga catacccctt
     6481 attgctttcc acagcaacac catgccaagg ggtcatccag ctttcattcc ctatcaaccg
     6541 aatccctaat ttttttgatc cgcaagtttg tggatgaata gacagccgaa ctgccttggg
     6601 aaagtgttcg gcaatgagcg cactccaggc gttgcttctt cttatgactt cataggcttt
     6661 aacacgagat tctttttgga tagcagtacg acttttagct tgacctgcat gcatggcatc
     6721 ttcaaacaaa aaacgcgtaa ttccgctata catacgatta gcttcttgtt catcggggct
     6781 tgctgagggt tttgcaccat tacggatctt ttgtttaagg aagtccaggg actgtccata
     6841 gcttttcatg agttcctcac gcatttggac aaaatgaaga tttttataaa aataatcaag
     6901 attaaaagtg gacagatctg caagggacat ttcttttata agtctgtcca gctcaagctg
     6961 gtaggctgtg acatcacttt cattcattcc aaccacatcg ctaaaaaccc ttccatcgga
     7021 acacagaata attttaatcc ccggggtata aaaatttttt attcgctgac aaagtgctcc
     7081 taaaaaatta agagaaaggc gttcagcatg atctggcagg tgacccagaa ctttttcagg
     7141 gttgggagat tttcccggga aggccggcaa tataaaagta accggttcat tctcattaac
     7201 tgccgagatg atttttggca aatggggtga agcgcacttt tggcatccgg tgctgcataa
     7261 gctaatggtt tccgggacac ggcgaaaaat cattacttca gctaatattc ttttggctac
     7321 ccccatagca gagtcactat cgggtgattt tttaagtcga taatctttag ggtaatgatt
     7381 ctctgtaatt aaaaacgctg tattcttcat tttccttccc tcctgaaaaa gagagtacag
     7441 atcttcgaaa aataattaaa atgatttatt ttaattgttg tgataaattt tacttatacc
     7501 taaaatggag ttgatgagtt atgtctttat tgttggatga tataaggtat ttcatagcgg
     7561 ccagcgatac gctcaatatg acaagagctt ctgaaattat tggaatatct caacccgcgc
     7621 ttagttattc tataaagaga ttggagaaca aacttggtgg attactattt atcaggctta
     7681 aaaatggaat ccagcttaca aaacttggag aagagtttaa gaggcgctct caccgtctgt
     7741 tatatgaatg ggagcaggtg caaaacatag caaaccctga atcaggtttt gttcagggaa
     7801 actatactat agcccttcac ccttccgttg cgctttacac tctccagtac tttatgccaa
     7861 aactgcaagt gaactatcct gggcttggca ttaatttcat tcatggtcat tcaagagaga
     7921 tgactgaaaa agtgataagt tgggaagccg attttggtat tgttgtgaac cccatacaac
     7981 accctgatct cgttataata aagctgtcta cggatgaagt gacagtcttt tatgccaatg
     8041 atgcacaaaa caaactgatt tatgatagaa aacttgccca atcacaatac atccttaaaa
     8101 aaattagcaa aaaaataact ttcaatggcg taattaattc taccagtctt gaagttgtag
     8161 ccaaactcac agccttaggt cttgggtatg gcattctacc taccagggta acctatccct
     8221 acaaacatct caaaaaaata ctcaatgcac cggtgtttag agatgagatt tgtcttatct
     8281 accgacctga aaaacataat aatcctgtca gtaa
//
LOCUS       DAXLDT010000129         8290 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXLDT010000129 DAXLDT010000000
VERSION     DAXLDT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.129"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1715
                     /locus_tag="ACF2SP_002367"
     CDS             <1..1715
                     /locus_tag="ACF2SP_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360141.1"
                     /translation="VEYEEFFKNRLNSSPLKYKLEEHCEKIPSVIGVCSLSSLFKQVF
                     PNDFVAEYYQKQDSCGDLILKNPLTGLLQENALLKPAEVMEKLKAFEKEYGDSVGLNL
                     FVHSKIINFLDEARTKYNYHQHYTRLMKVKSGMNEATMLELFESKEYSVVPVAYIITE
                     DNKLYFYHKENHPKLKAVPIDETNFQEMIKTATQQISKSGKKPEEELSLDNEQIKVVC
                     FLDLETLNNFCTLASHAPYSPGELNNIMNLLVLREIYIQYLSKILSSDKVLAAKTWCS
                     LKRLHLNFLDVVQKDVPISSTAREVIMKLDAAEKDYREHQNQRNLSFQKLSTALTSAA
                     KGILEKGYSFFKPANIKDIVSAYFSKEPEEQCGEKGYRQENHEALGFKLSIFHAFSGV
                     GDRWISYERTKPPVKEIDEFDWKFNLSIHKDDLSKAFPIIAEVANQMNLGLFKIMSQA
                     QANSVQKIDSKTMIGRELVIYCNANPELDAEKWIDILVQIENCFKKAGIRTSTATCPA
                     SNRKLGKYVSYTHGAWTNVRMDVPFAEGIKETALEDDDLFVNYEYDEAAEAPRKKMAS
                     KKLN"
     gene            complement(2167..2973)
                     /locus_tag="ACF2SP_002368"
     CDS             complement(2167..2973)
                     /locus_tag="ACF2SP_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HFD2360142.1"
                     /translation="MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFT
                     YQNEKLQSRVETMASEFNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAP
                     ADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTEGSILTLSYYGAEKAV
                     PNYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYA
                     NITPLQRNVTADEVGNTAAFLCSDLASGITGEVLHVDAGYHAVSAMNELG"
     gene            complement(3069..3707)
                     /gene="udk"
                     /locus_tag="ACF2SP_002369"
     CDS             complement(3069..3707)
                     /gene="udk"
                     /locus_tag="ACF2SP_002369"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFD2360143.1"
                     /translation="MTKQAIIIGISGPSASGKSLLANTIVNELGSEQVVVISEDAYYK
                     DNGHLPFAEREKINYDHPDSFDHALLYEHLRQLRVGNTVQIPIYSHSKHLRLPETRAV
                     GQHAIIVLEGILLFSDKALREIMDIRIFMSTPLDVCLTRRLKRDVVERHRSFESVIHQ
                     YETTVRPMYMQFIEPSSRYADIIVPRGGENRIAIEMIQAKMRELLASHTRGS"
     gene            complement(3760..5397)
                     /locus_tag="ACF2SP_002370"
     CDS             complement(3760..5397)
                     /locus_tag="ACF2SP_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFD2360144.1"
                     /translation="MKKISCSILLLINSLISYADSDQPKLILQIVVDQLRGDLIYRHH
                     KQFGPSGFNYLLAHGLDYHNAHHPHANTTTCAGHSTIATGSYPSLHGIVDNDWYDRKT
                     RKLKYCMEDLQAKILPTVHTQVEISGRSPRNLKPSTLSDEIILANKGKAFGVSLKDRA
                     AITLAGHAGKAFWFDKTNGGFITSNYYYSSYPLWVENWNKHYQPKAYDWNLSHPKSFY
                     QNANTLPFRHNYGSFGQTFPHHVTNPPSEEYFKSLSRTPKADELSADFAKQLLVNEKL
                     GKTTGQTDYLAISFSAVDAIGHQFGPNSLEAEDNLIALDDTLSQLFKTIDKEVGLNNT
                     LIILTADHGVSDGPSYLKAHKIPEIKPVNIEATGKHIENLLLKRFNLPAQALMSINPP
                     FIYLDHQIIKEKNLDIAEVSFYLAEEISHLPGIFKAYPLPIRDIEKDWLSAKVDRMSY
                     AYRAGDVYIVQPPYQSHGAKSEDRVAHGSPWQYDTYVPLLFVHPDFKPKRIFRQVHTT
                     DIAPTLSSLLLIKPPAAAVGQPLAEVVNAFHKTGEND"
     gene            5767..6429
                     /gene="lem14"
                     /locus_tag="ACF2SP_002371"
     CDS             5767..6429
                     /gene="lem14"
                     /locus_tag="ACF2SP_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem14"
                     /protein_id="HFD2360145.1"
                     /translation="MSFELVAYEKLKGSIRESIITLIKSHNEKAKIIEDKLEDSVKEV
                     SRERQPQVLVLLKTIELLDHSSKEPEDKARVLNALAYYIRDQIAATYKYTSPDNSDFY
                     KSLTISLDLNKDNNPNREDLADMYGALEKFLRSHVYKNSDPRKGYLDKQPFAIKHYSV
                     VDDILELSDRVHKLRHEIIIAARDLHLLQQKPKSSQGGLFSVPSGKEKVTETQEALTN
                     RL"
     gene            complement(6463..6813)
                     /locus_tag="ACF2SP_002372"
     CDS             complement(6463..6813)
                     /locus_tag="ACF2SP_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFD2360146.1"
                     /translation="MTKHKIKTIDVHELKHQMDNQANLSLIDVRELDEWEMMHIPGAL
                     HIPKDRISIEIQNQIPNKEQTIYLHCRSGVRSLYAAQCLMDLGYYEVYSVDGGIMAWA
                     MSGYPVKQESYTPS"
     gene            complement(6800..7081)
                     /locus_tag="ACF2SP_002373"
     CDS             complement(6800..7081)
                     /locus_tag="ACF2SP_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360147.1"
                     /translation="MLSEFAKQFSTQIQTFFYLIMLINGLLHFIFAGAVARDAGNLYR
                     LGQKPVLVSAPTWAFATLIGGVITATIYWLLHHSTLTRPTMREIRYDKA"
     gene            complement(7085..7981)
                     /locus_tag="ACF2SP_002374"
     CDS             complement(7085..7981)
                     /locus_tag="ACF2SP_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFD2360148.1"
                     /translation="MLHFKTIQSILLLIVLGFIWGSGYTLAKFAMTNGVNPLGYAFWQ
                     SLGPALLLTLVSFFTKQTRLLHPVYWPYFFICGLIGIAIPNTNMYFIASHIPAGLLAV
                     LVNTVPLLVYPLALMAGQEKLDGWRFFALLLGMSGILAIIGINSAGLYSSWTILAMLS
                     PLGFALCSIYIGAKQPKEINALQAANGMLLAASLLLTPVVLKQHAFYSLSAPLTLVKQ
                     IVILEIILSSLGYLLFFILIRMAGPVFYSLTGGVVAITGLFWGYLAFDEKPSSIQGMA
                     IALVVSAIFLLSWRQSKQTQGI"
     gene            complement(8070..>8290)
                     /locus_tag="ACF2SP_002375"
     CDS             complement(8070..>8290)
                     /locus_tag="ACF2SP_002375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360149.1"
                     /translation="LIHYYYSFHDDNIYRKERHLYNSRVSQQHIVNTALFIPTSILHV
                     LDQDFWLNVLQSYKIIIQHLQGLKKMFY"
BASE COUNT         2686 a         1491 c         1580 g         2533 t
ORIGIN      
        1 gagtagaata cgaggagttt tttaaaaata gacttaatag ctcaccctta aaatacaagc
       61 tcgaagagca ttgcgaaaaa atcccctctg taataggtgt gtgctcatta tcgagcctct
      121 ttaaacaagt attccctaat gattttgtgg cagagtatta tcaaaaacaa gattcctgcg
      181 gagatttaat actaaaaaat ccacttacgg gcttgttgca agaaaatgct cttcttaaac
      241 ctgctgaagt catggaaaaa ttaaaggcct tcgaaaaaga atatggggat agcgttggtt
      301 taaacttatt tgttcactct aaaataatta attttcttga tgaagcgcga acaaaataca
      361 attatcatca acattacact agattaatga aagtcaaatc aggtatgaat gaagcgacca
      421 tgcttgagtt gtttgaatca aaagaatata gcgtagtacc agtagcttac attattactg
      481 aagataataa actctatttt taccataaag aaaatcaccc aaaactcaaa gcagttccta
      541 ttgatgaaac aaattttcaa gaaatgataa aaactgccac ccaacaaata agtaaaagcg
      601 gtaaaaagcc tgaggaagaa ctttctttag ataatgaaca aatcaaggtt gtctgctttt
      661 tggatttaga gaccttaaat aacttttgta ccctcgcaag ccacgcccct tattcaccag
      721 gggaattaaa taacataatg aatttactcg tgttaagaga aatttatata caatatcttt
      781 ctaaaatctt gtcatcagat aaggttttag ctgcaaaaac gtggtgtagt ctcaaacgtt
      841 tacatttgaa ttttttagat gtcgtacaaa aggacgtccc tatttcctct acagcacgtg
      901 aggtcatcat gaagcttgat gctgcagaaa aagactatcg agaacatcaa aatcagcgta
      961 atctatcctt tcagaaactc tcaactgctt taaccagcgc tgcaaaagga atcttggaga
     1021 aaggctattc attttttaaa cccgctaata tcaaagacat cgtcagcgct tacttttcca
     1081 aagaacctga agagcaatgt ggagaaaaag gttatagaca agaaaatcat gaagccctcg
     1141 gtttcaaact ttcaatattt catgcgtttt ctggagtagg ggaccgttgg ataagttatg
     1201 aacgcaccaa gccgccagtt aaggaaatag acgaatttga ctggaaattc aatttatcca
     1261 ttcacaagga cgacctgtcc aaagcgtttc ctattattgc cgaagtggct aatcaaatga
     1321 atttaggctt atttaaaatt atgtctcaag cacaagccaa tagtgtacaa aaaattgact
     1381 ctaaaaccat gatagggcga gaactcgtga tttattgtaa tgcaaatcct gagcttgatg
     1441 ctgaaaaatg gatagatatc ctggttcaga ttgagaattg ttttaaaaaa gcggggattc
     1501 gaacttctac tgctacatgc cctgcttcca atagaaaact cggcaaatat gttagctata
     1561 ctcacggtgc atggactaat gtacgtatgg atgttccctt tgccgaaggg attaaagaaa
     1621 ctgccctaga ggatgatgac cttttcgtca actatgagta cgatgaagct gctgaagcac
     1681 ccagaaaaaa aatggcatca aaaaaactaa attaatggag ggggcaaatt aattcaataa
     1741 gagtggcagc cgagagtctg gcgaaaattc acattctctg tttgtttgta gttcagattt
     1801 tgtttcactg tcaattagtt atttttagtt aatcgaaata aaccaaaacg attaagatag
     1861 ggtgcatgac atttttaaat gcagaataga gtcaataaat agttatttga agcagtccaa
     1921 gagcatcagg gtaattttat ggaaaaaatt ttggcacatt ctcgtattgt ggttctgatt
     1981 tcttgttcag aaccatctca cttactgttt tttgcagatt gttagaaatt agtgcttttg
     2041 aagtatttta tcagattttt agaattgatt tttcgttcaa aaaaaaatca tctttgcaaa
     2101 tactaattga cctattacaa tacagcaata aatttaatca gcttcttaaa gctgattaat
     2161 atttatctat cccaattcat tcattgcgga tacagcatga tatcctgcat caacatgtag
     2221 cacttcacca gtaatcccgg atgccagatc agagcataag aaggcagcag tatttcctac
     2281 ttcatcagca gtaacatttc tttgtaatgg agtaatgttt gcataggcag catgaatttt
     2341 acgaaaatct ttaattccag cagcggcaag agttttaatt ggtccagctg atatggcatt
     2401 aattcttaat cctctggatc ctaagctggc tgccagatag cgcacagagg cttccaagct
     2461 tgccttcgca acacccataa cgttatagtt tggtacggct ttttctgctc cataataact
     2521 taaggttaaa attgaaccct cagtatcaag catcataggc aatgcggctt gagacaaacc
     2581 tatgaggcta taggcactaa tatcatgagc tattctaaat ccttctctat ttgcgcattc
     2641 tacaaaatca ccactgatct gatcagcagg agcataagct acagagtgaa ccaggatatc
     2701 taatttatcc cagtgattac gcaaattatc aaacactgct tttatttctt catcacttgc
     2761 aacatcacaa ggaaaaacca gggttgaatt aaattccgat gccattgttt caacacgtga
     2821 ctgtaatttt tcgttttggt aagtaaaagc caactcagcg ccttgattat ggaatgcttt
     2881 agcaatgcca tatgcaatcg atctgttgct tgccaatcca acaataagtg ctttttttcc
     2941 agttaaaaat cccacaatag tatctcctcc cattattctt aatttagcag ggcgaagtga
     3001 gtgttacttc gatcctggac taaaaccaca atatcaatta ggttgctctt aaacagccta
     3061 ataaaacatc aactgcctcg agtatgtgaa gctaataatt cccgcatttt ggcttgaatc
     3121 atctctatag cgattctgtt ttcaccgccg cgaggtacta ttatatcagc gtagcggctt
     3181 gaaggctcaa taaattgcat gtacataggg cgaactgtag tctcatattg atgaataacc
     3241 gattcaaatg atcgatgcct ttctactaca tctcttttta agcgtcgcgt taaacagacg
     3301 tcaagaggag tcgacatgaa tatacgtata tccatgattt cgcgtagagc tttgtcactg
     3361 aaaagtagta ttccttcgag tacaataatg gcatgttgac ccactgctct ggtttctggt
     3421 aaacgcagat gtttggaatg agaataaata ggaatttgta ctgtatttcc aactcgtagc
     3481 tgacgaagat gctcataaag tagagcatga tcaaaggagt caggatgatc gtaatttatt
     3541 ttttctctct ctgcaaaggg tagatgccca ttatctttat agtaggcatc ttcagagata
     3601 acgacgacct gctcggatcc taattcattg actatagtat ttgctaataa actttttcca
     3661 gatgctgatg ggccggaaat cccaataata attgcttgct ttgtcataat gttttcatta
     3721 aaaattttgt acgaatagta aagttttttc ggttggaatt caatcattct ctccagtctt
     3781 atgaaaagca ttgactactt cagccaaagg ctgtcccact gctgcggcag ggggtttaat
     3841 caataataag gaagataatg ttggtgcaat atccgttgtg tgtacttgcc taaatatacg
     3901 ttttggtttg aagtcagggt gtacaaacag tagcgggaca taagtatcat attgccaggg
     3961 actgccatga gcaactcgat cttcactttt tgcaccgtgg ctttgatatg gcggttgaac
     4021 aatgtaaaca tcgcctgcac ggtatgcgta agacatgcgg tcaacttttg cgcttaacca
     4081 atctttttca atatcacgaa taggtaaggg gtaggctttg aatatccctg gaaggtgact
     4141 gatttcttct gccaggtaga aactaacctc tgcaatatcc aaatttttct cttttataat
     4201 ttggtgatca aggtatataa aaggaggatt aattgacatt aaagcttgcg caggcaggtt
     4261 gaaacgtttc aataataaat tctcgatgtg ttttcctgtt gcttctatat taacaggttt
     4321 aatttcaggt attttatgtg ctttaaggta agaaggacca tcgctgacac catgatctgc
     4381 ggtgagtata attaaggtat tattaagacc aacctcttta tcaatggttt taaaaagttg
     4441 gcttagggtg tcatcaaggg cgatcaaatt gtcttctgct tccagactat tagggccaaa
     4501 ttggtgacca atagcatcta cagcagaaaa actgattgca agataatctg tttggcctgt
     4561 tgtttttccc aatttttcat taaccagtag ttgtttggca aaatcagctg ataattcatc
     4621 tgcctttggt gttctggaaa gcgatttgaa atattcttca gatggaggat tggttacatg
     4681 atggggaaat gtttgaccaa agctgccata gttatgacga aatggaagag tatttgcatt
     4741 ctgatagaat gatttaggat gagaaagatt ccagtcgtag gctttgggtt gatagtgttt
     4801 attccagttt tctacccata gtggataact ggaatagtag taattgcttg taataaatcc
     4861 accatttgtt ttgtcaaacc aaaacgcttt tcctgcgtga ccagccagag tgatggctgc
     4921 cctgtctttt aatgatacac caaaggcttt ccctttattt gctaaaataa tttcatcgct
     4981 taatgtggat ggttttaggt ttcgcggtga gcgtcctgaa atttctacct gggtgtgtac
     5041 agtgggtaaa attttggctt gtaaatcctc catacagtac tttagttttc ttgtttttct
     5101 atcataccaa tcattatcaa ctatgccgtg taaagagggg tagcttcctg ttgcaatagt
     5161 gctatggcct gcgcaggtcg ttgtattggc atgtggatgg tgtgcgttat gataatccaa
     5221 accatgagca agcaaataat tgaaaccaga aggtccaaac tgtttatgat gacggtaaat
     5281 cagatctccg cgtaattggt caacaactat ttgtaaaatt aatttaggtt ggtctgaatc
     5341 agcgtagcta attaaggagt tgatcaaaag aagaatactg cagcttattt ttttcatttt
     5401 tatgaccatc atatattgca aattgacgcg taaattaaat caaatgtttt ataaaaacaa
     5461 taaatagctt atttaaattt cttctaattt ttattgtgtc tgactgattg agcaaaaaat
     5521 atcttgattt aattgatctg agcttactct taaagcgacg ataacaaggc ttaaaaatac
     5581 tctccagcag tctattcatt tgatgaacta acaaagaaat tgcaaatttt tcattaaaat
     5641 caatagattg aaaattaatt agatagtaaa aagaaaaaac atgacgaaaa gagggaattg
     5701 gtttaaactg gcctacagtg aaagcatttc actagtcctg ttcatggagt ataaggagaa
     5761 aataagatgt catttgaatt ggtcgcatat gaaaaattaa aaggaagcat cagggaaagt
     5821 atcattactt taatcaagtc ccataatgag aaagccaaaa ttattgagga caaattagag
     5881 gactcagtaa aagaagtttc aagagaaagg caaccacaag tacttgtctt gctaaaaact
     5941 atcgaattgc tggatcattc atccaaagaa ccagaagata aggcacgcgt attaaatgca
     6001 ttggcctatt acattcgtga tcagattgct gcaacttata aatacacttc tcccgacaac
     6061 agcgattttt ataaatctct taccatatcg cttgacttga ataaggataa taatcctaat
     6121 cgagaagatt tggctgatat gtacggtgct ttggaaaaat tcctgcgttc tcatgtttat
     6181 aaaaattctg acccaagaaa agggtatttg gataaacaac ctttcgcaat caagcattat
     6241 tctgttgttg atgatattct tgaattatct gatagagtgc ataaattaag acacgagatt
     6301 attatcgccg ctcgcgattt gcatttactg caacaaaaac caaagagctc tcaaggcggt
     6361 ctttttagtg ttccatcagg aaaagaaaaa gtcactgaaa ctcaggaagc cctgactaat
     6421 cgcctttaag caactcattc tcttttattc tcacctcttc actcaggaag gggtgtaaga
     6481 ttcctgtttt accggataac cactcattgc ccaagccata atgccaccat ccacagaata
     6541 gacttcgtaa tatcccaaat ccattaagca ttgagccgca tacagtgatc tgacgccgct
     6601 tcggcaatgt aaatagatag tttgttcttt attgggtatt tggttttgta tttcaataga
     6661 gatgcgatct ttaggaatat gaagtgctcc cgggatatgc atcatttccc attcatccag
     6721 ttcacgtaca tcaatcaaac tcaaattggc ttgattgtcc atttggtgtt ttagttcatg
     6781 aacgtctatt gttttgatct tatgctttgt cataacgaat ttctctcata gtgggtctgg
     6841 ttaatgtgga gtgatgaagc agccagtata tagttgccgt gatgactcct ccaattagag
     6901 tcgcgaatgc ccaggtaggg gccgatacta aaacaggttt ttgacctagc ctatagagat
     6961 tacctgcgtc tcgagccact gctccggcaa aaataaaatg taaaagacca ttaattaaca
     7021 taattaaata gaaaaaggtt tggatttgtg tgctaaattg ttttgcaaat tcactgagca
     7081 tgaatcagat tccttgagtc tgtttcgatt gtctccatga taacagaaaa atggcggaaa
     7141 ccactaaagc tattgccatg ccctgtatag atgaaggctt ctcatcaaaa gccaagtacc
     7201 cccaaaacaa tccggttatt gcaacgactc caccagttaa actataaaaa acaggtcctg
     7261 ccatacgaat gagaataaag aaaagtaaat atcctaaact ggataaaatt atttcaagga
     7321 taacgatttg tttcacgaga gttaacggag ctgagagaga ataaaatgcg tgctgcttga
     7381 ggacaacagg agtaagcaga agagacgccg ccagtaacat tccattagca gcttgcaggg
     7441 cgttaatttc ttttggctgt ttggctccta tataaattga gcacaaagca aagccaagag
     7501 ggctaagcat agctaatata gtccagcttg agtaaagtcc tgcggagttt attcctataa
     7561 ttgcaagtat gcccgacata ccaagtaaca aggcaaaaaa tcgccaccca tccagttttt
     7621 cctggcccgc catgagagct aaaggataaa ccagtaaagg aacagtatta actaacactg
     7681 ccaataaacc tgcagggata tggctggcaa taaaatacat gttggtatta ggaatagcga
     7741 tacctatcaa tccacatata aaaaaataag gccaataaac agggtgtagc aagcgagttt
     7801 gttttgtaaa gaagctcact aaagttaata gtagggctgg ccctaatgat tgccaaaaag
     7861 catagcctag tgggttaact ccattagtca ttgcaaattt ggcaagagta tagcctgatc
     7921 cccagatgaa tcctaaaaca attagaagaa ggattgattg tatggtttta aaatggagca
     7981 taaaaggtag gataaatagt ataattttgg ataattatgc cattgaaagt agaaaataac
     8041 gatatccttg tgaggccttt aaattttgct caataaaaca ttttttttaa tccctgcaaa
     8101 tgctgaatta ttattttata tgattgcagt acatttagcc aaaaatcctg gtcaagaaca
     8161 tgaagaatcg atgttggaat aaataatgct gtattcacaa tatgttgttg tgaaacacga
     8221 ctattatata aatgcctctc ttttctatat atgttgtcat catgaaaact gtagtaataa
     8281 tgtattaaga
//
LOCUS       DAXLDT010000130         8166 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXLDT010000130 DAXLDT010000000
VERSION     DAXLDT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.130"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(45..290)
                     /locus_tag="ACF2SP_002376"
     CDS             complement(45..290)
                     /locus_tag="ACF2SP_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360150.1"
                     /translation="MNSWNSVVFLKTKNAWSDSAAIAKMNGVQSLWSTSGDWDWCIKL
                     DSKHSSPEETALFVKNLRNADWVAETKTSWWKEVAVK"
     gene            complement(720..2048)
                     /locus_tag="ACF2SP_002377"
     CDS             complement(720..2048)
                     /locus_tag="ACF2SP_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFD2360151.1"
                     /translation="MTKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIK
                     QNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQMPVAVITAYGNVEGAVNTLKAGAFDYV
                     SKPIDLKMLRDLVKTALSMQNPPTEKPDEDLLVGESLVMQQLRENIHKLARSQAPVFI
                     QGASGVGKELVAQLIHVNGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDK
                     TGLFVAAHGGTLFLDEIAELPLAMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHK
                     NLIEEVNAGAFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKI
                     NPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTS
                     TNLNGYLQDQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD"
     gene            complement(2045..3631)
                     /locus_tag="ACF2SP_002378"
     CDS             complement(2045..3631)
                     /locus_tag="ACF2SP_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2360152.1"
                     /translation="MPAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKY
                     YPLLFLCIAFAYLLFALIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGIIGNLESG
                     YGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
                     AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRI
                     RLMNTAAREFFNLSKHHAIQKLDQLSDSLVSKFDDFLLKTTNKERTAQTIIEDPYLRV
                     HFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQ
                     LFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELTTFLEHFKH
                     DFTHNNPCDLTIKLPVNKPLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAK
                     CSSNKITLTVCDSGPGIPSEHRDNIFEPFFTTLRGGTGMGLFIARDLCEINQARLNLV
                     KSNKGCCFAITVNPSDELLI"
     gene            complement(3730..5214)
                     /gene="lidL"
                     /locus_tag="ACF2SP_002379"
     CDS             complement(3730..5214)
                     /gene="lidL"
                     /locus_tag="ACF2SP_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LidL"
                     /protein_id="HFD2360153.1"
                     /translation="MQGGMMKQLVVAGFFLLLLTSPLSALDVDTYRSSQKQFNTEDKA
                     EQGDAQAQFELGLKYEKGDGVNKDLKKAIYWYQKAADQGQAEAQNNLGVLYLKGEGVP
                     QNSQQAMYWFKKASEQGLAIGQNNIGILYENGLGVKKDLGQAFIWYQKAAEGGNSDGQ
                     YNLAVMYMYGNGIPKDIKKAIHWYIKAAEQGDLDAQNNLGVLYERGEEVPRDLKAAIS
                     WYTRAANEGSLIAQTNLGVLYMTGDPSIQDGKKAIYWYEKAAAQGGEKAQNNLGYIYE
                     QGIGTEKDMKKAIYWYEKAAENGFTLAQNNLGVLYSNDGELQDYKKAYLWFKKAADQG
                     FAEAQNNLGLMYMKGNGLSVNYHEAVLWYKRAAEQGLPLAQHNLAIMYMKGLGIKKDN
                     KLAIKWYQKAAEKGLDLAQNNLAVMYIRGEGVKRDFKKAMYWYQKAAEQGLDLAQINL
                     GIMYLDGMGVNKDFAKAKYWIGKAKDSGSQDALTIWNKNKLWKY"
     gene            complement(5364..5783)
                     /locus_tag="ACF2SP_002380"
     CDS             complement(5364..5783)
                     /locus_tag="ACF2SP_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1171 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360154.1"
                     /translation="MRFQDSDFEERYNTMWNKIAVSADAQIRQLFGAKGFFSEQQPNY
                     YQLLVNYAQAAKNIVDNLNRQSPMFDDKEYVEGYMIATLQSVYKDFSQYKPRIAGRYG
                     EHSSCVELINKTLDWVQSFDLKLENLSESDDEMKITF"
     gene            complement(5842..6918)
                     /gene="amrS"
                     /locus_tag="ACF2SP_002381"
     CDS             complement(5842..6918)
                     /gene="amrS"
                     /locus_tag="ACF2SP_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HFD2360155.1"
                     /translation="MFDTIPTKYWHKLEDGRIQCDVCPRNCKLREGQRGLCFVRANLD
                     NQIKLTTYGRSSGYCIDPIEKKPLNHFLPGSPVLSFGTAGCNLACKFCQNWDISKSRD
                     IDTLADTAMPEDIARAAQNMNCLSVAYTYNDPVIFMEYAIDVAKECHKLGIHSVAVSA
                     GYINPEPCKELYSFMDAANIDLKSFSEDFYYKITGSHLQPVLDTLIYLKKETDVWFEI
                     TNLVIPEENDSDVEFERMSEWIMTNLGPDVPLHFTAFHPDWKMMDKAPTPFSTLQRAR
                     EIAIKTGLHYVYTGNVHDTIGSSTYCPHCKLPVIVRDWYRILDYRVDDTGHCLNCHTQ
                     IAGVFKGAVGHWGPRRVMVKLGCS"
     gene            6990..>8166
                     /gene="amrB"
                     /locus_tag="ACF2SP_002382"
     CDS             6990..>8166
                     /gene="amrB"
                     /locus_tag="ACF2SP_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HFD2360156.1"
                     /translation="MSVRQPAVAGYFYPDDPLTLKQTVLNFLDQAPIHKPAPKAILVP
                     HAGYVYSGSVAASAYASLRDKKNSINKIIILGPAHRLYFKGIAYDPVDKFATPLGEIQ
                     QDKELLTQIIDLPYVYSLPEAHQNEHCLEVQLPFCQMIFSKFKILPLVVGETNPQDVA
                     RLIARVWGGDDTLLIISSDLSHYLPYHIAQREDKKTCLSIDTLNGEEILHDAACGYFP
                     LRGFLYFARQHHIYSRLLDLRNSGDTAGDKERVVGYAAYHFYQKLNFSEHCADELKYI
                     AKETIRLQTEENKALAINYNDYNQLLQIRIPTFITLKKNGLLRGCMGSLIAKERLADN
                     VIYNSIRAAIADPRFPQIRPSELKELSLTISLIKPLSPLYFDSEEELKSQLQIGIDGL
                     "
BASE COUNT         2550 a         1734 c         1327 g         2555 t
ORIGIN      
        1 tataaaatac cattataatt tcccaaacaa ttcatcttat taaattactt tacagcaact
       61 tctttccacc aactggtttt ggtttctgca acccaatcgg catttcttaa atttttaaca
      121 aacaaagcag tttcctcagg gcttgagtgc ttggagtcaa gttttataca ccagtcccag
      181 tctccagagg tagaccaaag gctttgaaca ccattcatct ttgctattgc agcagaatct
      241 gaccaggcat ttttagtttt taaaaaaaca acactgttcc atgagttcat tgtttcttcc
      301 ttaattttaa tcaattatgt tttttcgcat tgaacataaa ttcattgcgt tatataaaat
      361 tagactatga attatcagat ttcaaactcg aatgaaaaac aattctgata ttaggccttt
      421 tgcctaagct aattattttg tttcacggca aggcgaaaac gttttgagga acagatttta
      481 catacacagg acttacgaaa aactccaagg tcgaggcaaa aaatgttttt aatgagggaa
      541 tttagataaa ctaaatgacc gaattaaaaa cattttttaa catagagatt ggggtttttc
      601 ataagtcctg atacagtaaa tgagcacctc agaaacgttt tcagggctac aataaaacga
      661 aaaaggctgg ctaggcaagc ggtctatttt aacctttgaa aatcttcaaa gcgcaacatc
      721 taatcaaggc ccagtttttt taatcgataa cggagagttc ggaagctgac ccccagtagt
      781 tgggcagcag cggttctgtt ccattttgtt ttctcgagcg catcaagaat taactctttt
      841 tcctgatcct gaaggtaacc atttaagtta gttgatgtgg agggtacggc aacagctggg
      901 gttattgttg ttctgggcaa atgcaaatcg gatggttcta taacctctcc ttcacacata
      961 gccatggcac gctcgaggat attctccagt tctctgacat ttcctggaaa atgataacga
     1021 ctcaatattt caaaacagga tggatttatt ttcaccaggt ttctgttttg atttttagat
     1081 aattttctta atatcgcctc tgtcaggcaa ggaatatctg aaagcctttc cattaaagtt
     1141 gggacacgca attcaatcac attaattcga taatacaaat cttgacgaaa tgcccctgca
     1201 ttaacttcct caatcaaatt tttatgactc gcacttagga tgcgaacatc cacaggaact
     1261 tcatataact cacctatcgg cttaattgcc ttctcctgaa tggcacgtag caattttacc
     1321 tgcatggcga gaggaagttc ggcgatttca tcaagaaaca aagtgccacc atgagccgca
     1381 acaaacaacc ctgttttatc agaaatcgct cctgtaaagc tccctttttt atggccaaaa
     1441 aactctgatt ccatcaactc accaggtatt gcgccacagt ttacaggaat aaatggctta
     1501 tcctttctgg gaccattaac atggatcaac tgagcgacca attctttccc aacacctgaa
     1561 gctccttgaa taaaaactgg tgcctgactg cgagctaatt tatgaatgtt ttcacgaagt
     1621 tgttgcatta ccaaactttc accaaccagt agatcttcat caggcttctc ggtaggcggg
     1681 ttttgcatgg ataatgccgt cttaacgaga tctctaagca tttttaagtc tataggtttt
     1741 gatacgtaat caaatgcacc cgcctttaaa gtgttgaccg caccctcgac atttccatag
     1801 gcagtaatga cggcaactgg catctgcggt ttgtatttct gaatgaattt aacaatttct
     1861 ataccatctc catcaggtaa acgcatatcg gttaaaacca aagaataata attttgtttt
     1921 atatgttcta tgccttgctt aaagtcagac actgcatcgc aagttaaacc catacgtgat
     1981 aaagtaagag ttaataactc tcttatatct ggttcgtcat ctataacaag cactttactt
     2041 ttggtcatat taaaagttca tccgatggat taacagtgat cgcaaaacaa catcctttat
     2101 ttgattttac caaatttaat ctggcttgat taatttcaca taaatctctg gcaataaata
     2161 accccatccc tgtaccacct cgcaaggtgg taaaaaatgg ttcaaatata ttgtctctgt
     2221 gctcagaggg tataccaggt ccagaatcac atacagtcaa agtgatttta ttcgaagaac
     2281 atttggcagc aatgacgata ttcacattac cctcatgatc ccgcccatgt tgaatagcat
     2341 tttcacacaa gattactaaa atctgttcta attgactttt atcaaaaaca accgataacg
     2401 gcttatttac aggtagcttt attgtaagat cacagggatt attgtgagta aaatcatgct
     2461 taaaatgttc taaaaatgtt gtaagttcat taacctgcgg ttgagatttc tcacgccttg
     2521 aaagctgcaa tacattttta atcaccctgt tcattcgctc acaattgttc atgattaatt
     2581 gttttaaacg agtgttctct tcatttaaat cctcattatc tccaaacaat tgaatagcat
     2641 gagcaattgc ccccaaggga ttacgtaatt catgagcaat acttgctgaa aaacgcccta
     2701 aagctgctaa tttgagttgt tgtgcttgtt gtgcaatata agtcatgtct tccagaataa
     2761 tgagtacttc agacttgtga ccaacagaag tagaaaaaaa atgcactctt agataaggat
     2821 cttcaataat agtttgagct gtacgttctt tattcgttgt ttttaaaaga aaatcatcaa
     2881 atttggatac aagtgaatca gatagctggt ccaatttttg aattgcatga tgtttactca
     2941 gattaaaaaa ttcacgagca gcagtattca tcaaccgaat tcgcttcccc tctgcaacat
     3001 aaataattcc tgaatgcaat cgttcgacaa tatattcatt gagcctttgc attcctgcca
     3061 actcataact acggtgtctg gccagactct cagacattct gacccaattg gataaatacc
     3121 aggcggtgat agccgttgca aaaaaaccag ccccatagat tccactataa aaaaaagtgc
     3181 ttaagtcttt ttgattataa ataaaaaatt gcaaaacatt gccacatagc aataaacaac
     3241 tggctagtga tgcaaaatag atggccaagc gcccgggaac taaaatactt aaagcagcaa
     3301 tagtaacatt tagtaatatg ccataaccgg attccaaatt accaatgatg cctagcatgc
     3361 tggaaatggc tatgacatca atggtacccg ataaaaacac ctgcttatca aaacttaaaa
     3421 tttttctatg ccaaaaataa agaaaaatca aagcaaataa taaataggca aaagcgatgc
     3481 agagaaataa taagggataa tatttattga tataactgaa ataataaatg cctaaaaata
     3541 gaaaaatact tactattctg tatatattgt aaaccaacaa tattcgccat tgtctcattt
     3601 tcggattgtt gatttggcga gcagcaggca tccttttcta atccattaga ttattcagcg
     3661 tattttaatt atataaccag acttaagcat tttgcacaat acatcaacta agacccacac
     3721 caatgatcac taatatttcc acaatttatt tttattccat atagtcagcg catcttgaga
     3781 accactgtcc ttggcttttc caatccagta ctttgcttta gcgaaatctt tattaacacc
     3841 catgccatca agatacataa tacccagatt aatttgtgcc aaatccaaac cttgttcagc
     3901 tgccttttga taccaataca tggctttctt aaaatctctt tttactcctt caccacgtat
     3961 atacataacc gccaaattgt tttgcgccaa atccaatcct ttttcagcag ctttttggta
     4021 ccatttaatg gccagtttat tgtctttctt tatccccagc cctttcatat acataatcgc
     4081 cagattatgt tgcgctaaag gtaatccttg ttctgccgct ctcttgtacc acaagactgc
     4141 ttcgtgataa ttaactgaca gtccattgcc tttcatatac attaaaccca agttattttg
     4201 ggcttcggca aatccttgat ctgcggcttt tttgaaccat aaataagcct ttttgtaatc
     4261 ttgcagctcg ccatcatttg aatagagaac acctaaatta ttttgagcca gagtaaatcc
     4321 attctctgcg gctttttcgt accagtaaat agcctttttc atatcctttt ctgtaccaat
     4381 tccttgttcg taaatatacc caagattatt ttgtgctttt tccccacctt gtgccgctgc
     4441 tttttcatac caatatattg ctttcttgcc atcttgaata gaaggatcac cagtcatata
     4501 caatacacca aggttagttt gagcaatcag tgaaccttca tttgctgctc tggtatacca
     4561 actaatagct gcttttaaat ctcttggaac ctcttcgcct cgctcataaa gaacaccaag
     4621 attattttga gcatcaagat ctccttgctc tgctgctttg atataccagt gaatcgcctt
     4681 cttgatatct tttggtatac cattcccata catatacatg actgcaaggt tatattgtcc
     4741 gtctgaattt ccgccttctg cagccttttg ataccagata aaagcctggc ccagatcttt
     4801 tttaacaccc aggccatttt catatagaat tcctatatta ttttgaccaa ttgccaaacc
     4861 ctgttccgaa gccttcttaa accaatacat tgcttgctga ctgttttggg gcacaccctc
     4921 tcctttaaga tacaatacac cgagattatt ttgtgcctca gcttgtcctt gatctgcagc
     4981 tttctgatac caataaatcg ctttttttaa atctttattt actccatcac ccttttcata
     5041 tttgagccct aattcaaact gtgcttgagc atcaccctgt tccgccttat cctctgtatt
     5101 gaattgtttt tgtgaactac gataggtatc aacatccagc gctgataaag gggaagttag
     5161 aagtaataag aaaaatcctg caacaaccaa ttgtttcatc atacctccct gcaataattg
     5221 ttcaattata gcacaactga tgatgaataa acattaacgc tctggtgcca tacaagcaaa
     5281 gtgcatctat taggagtaag atataataga gaaatgttgc tatcccgcat tacagacttc
     5341 tcgtaataca ggatagcttg caattagaaa gtaattttca tctcatcatc agattctgag
     5401 aggttttcta attttaaatc aaaggattgc acccaatcca aggtcttgtt aatcagttca
     5461 acacagcttg agtgctctcc ataacgccct gcaattctcg gtttatattg agaaaaatct
     5521 ttatataccg attggagagt agcaatcata tagccctcaa catattcttt gtcatcaaac
     5581 atcggagatt gacgattcaa attatcgaca atattcttgg ccgcttgagc atagtttaca
     5641 aggagctgat agtaattggg ctgctgttca ctaaaaaatc ctttggcgcc aaataattgc
     5701 cttatttgag catcagcaga cactgcaatt ttattccaca ttgtattata acgttcttca
     5761 aagtcagaat cttgaaatct catattaatt caccatgtac aaaaacatga catataatac
     5821 taaaaattta ttatgtaaag attaagagca acctaattta accattactc ttcttgggcc
     5881 ccaatgacca actgccccct taaatactcc tgcaatttgc gtatgacaat ttaagcaatg
     5941 tccagtatca tccacacgat aatccaatat tcgataccaa tcccttacaa tcacaggtaa
     6001 tttacaatga ggacaataag tgctgctgcc gattgtatcg tgaacatttc ctgtataaac
     6061 atagtgtaag cctgtcttga ttgcgatttc tctggctctt tgtaaagttg aaaatggagt
     6121 cggagcttta tccatcattt tccaatctgg atgaaacgca gtgaagtgta aaggaacatc
     6181 aggccccagg ttagtcataa tccattcgga catcctctca aactcgacat ccgaatcatt
     6241 ttcttctgga atcactaaat tagtaatttc aaaccataca tcggtttcct tcttcaaata
     6301 aatgagcgta tccagaacag gttgtaaatg agaaccagta attttgtaat aaaaatcttc
     6361 actaaaggat tttaaatcga tattagcagc gtccatgaat gaataaagct ccttacatgg
     6421 ctcaggatta atataccctg cagagaccgc tactgaatga ataccaagct tatgacattc
     6481 ctttgcgaca tcaatggcat actccataaa aatcaccgga tcattgtatg tatacgccac
     6541 actgaggcag ttcatatttt gtgcagccct tgcaatatct tctggcatgg cagtatctgc
     6601 caatgtatca atatctctgg atttgctaat atcccaattt tggcaaaact tacaggccag
     6661 attacatccc gcagttccga aagataagac agggctaccc ggcaaaaagt gattcaatgg
     6721 ttttttttca atgggatcta tgcaataacc gctagagcgg ccataggtag ttaattttat
     6781 ttgattatcc aggtttgcac gaacaaaaca taaccctctt tgcccttcac gcaatttaca
     6841 attacgagga catacatcac attgtatgcg tccatcttcc agcttatgcc agtatttggt
     6901 tggaatagta tcaaacatca aatagcccac aatcaactct tagtctatat ttataagtat
     6961 agtatgttca gttgttccgg agtgttgttg tgagcgtccg tcaaccagcc gtagctgggt
     7021 atttttatcc cgacgatcca ttgactttaa agcaaaccgt attgaatttc ctggaccaag
     7081 cgccaattca taagcctgct ccaaaagcaa tactagttcc gcatgctggt tatgtctact
     7141 caggatctgt agcagcttct gcatatgctt cgttgcgaga taagaaaaat tccatcaata
     7201 aaattataat attaggcccc gctcacagat tatatttcaa aggcatagcc tatgatcctg
     7261 tcgataaatt cgctacacca ttgggagaaa ttcaacagga taaagagtta ctaactcaaa
     7321 ttatagatct gccttatgtt tattcacttc ctgaagctca tcaaaatgaa cattgtctgg
     7381 aagtacaatt acctttctgc caaatgatat tctccaaatt taaaatactt cctctggttg
     7441 ttggagaaac gaatccccag gatgtagccc gtctaatagc gcgagtatgg ggtggagatg
     7501 atactctcct gattatcagc tctgatttaa gccattatct gccctatcac atagctcaaa
     7561 gagaagataa aaaaacttgc ctttccatag acactttaaa tggtgaagaa atactgcatg
     7621 atgctgcttg tggttatttt ccactgcgcg gtttcctgta tttcgcacgc caacaccata
     7681 tttatagccg tctgctcgat ttgcgcaact caggcgatac agctggagat aaagaaagag
     7741 tagtggggta tgcggcctat catttctacc aaaagcttaa tttttcagaa cattgcgcag
     7801 atgaattgaa atacatcgcc aaagaaacaa tacgattaca aacagaggaa aataaagcat
     7861 tagcaattaa ttacaatgat tataatcaat tattacagat acggatcccc acttttatta
     7921 ctttaaaaaa aaatggtttg ctaagaggtt gcatgggaag ccttatagct aaagagcgat
     7981 tagcagataa cgtcatttac aattccatac gggcagcaat tgctgaccca cgcttcccac
     8041 aaataagacc atctgaactc aaggaattgt ctctaactat ctcattaata aaaccattat
     8101 ctccactata ttttgacagt gaagaagaac tcaaatcaca attgcaaatt ggcatagatg
     8161 gcttgg
//
LOCUS       DAXLDT010000131         8164 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXLDT010000131 DAXLDT010000000
VERSION     DAXLDT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.131"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..302
                     /locus_tag="ACF2SP_002383"
     CDS             <1..302
                     /locus_tag="ACF2SP_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calmodulin"
                     /protein_id="HFD2360157.1"
                     /translation="TACSNSIVSTIKLLKQLDNKRSFNPEQSLKNFCGYKEIMIQLLL
                     KPFETPVAEMASVPATTSDLIEKQSGIKTRLQEMKSSANQDKQPIFTPSLMRGPS"
     gene            661..1974
                     /locus_tag="ACF2SP_002384"
     CDS             661..1974
                     /locus_tag="ACF2SP_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFD2360158.1"
                     /translation="MEYHRVRQFSYGLVLLLLYASLATAGNALTLKEVTQIALANNKD
                     LKAARYNVSIAKARLLQAGFWPNPSLNLSNNDDRLFTDEGEYSRSAGFSQAFPISGRI
                     AKQKTVARLDVLKAITEIREAERQLSAKVASAFYTAVITEHRIQQVNYLLRINRELVD
                     VIHNRYHAAEISKLDDNAARIEYLRIVQEKHLLDSFRISQYATLNQLMGREANSSISL
                     KMDFATEKRLPALDTLKTQALKNRPDRQSIAFSIERANADRHLAKAERFADWTVGLGI
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAMMSLRALDLSI
                     ATEIASNYEQLKALRKSLEETQRVSLQLTIENVKLARDSYENGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKFLQVYVALCTAIGPGKTDGFCSYLEYQSNGDSK"
     gene            1971..2939
                     /locus_tag="ACF2SP_002385"
     CDS             1971..2939
                     /locus_tag="ACF2SP_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2360159.1"
                     /translation="MRLQLKIRMMIMTVFFLGLLSPMQVSFAHGGNIEVSEGGTKGPV
                     HLTPEQARMLDIKVVQATHRPIAQLLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DSVKAGQRLATVQSRLVGNPPPSVAVNAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVVAQVYEEDLGKVKVGQEVNVHVLSYPKQTFPGQVTLIEPNLDPLTRTVNVRITL
                     DNKEGLLKPGMFVRANVILTHNKAALTVPNAALLQADNQQFVFVQNGGTYKRVDVKVG
                     AVEDDYSEIIDGLVPGDLVVTQGNLELYTLWLSGGRKLQSGQEEHH"
     gene            2940..6140
                     /locus_tag="ACF2SP_002386"
     CDS             2940..6140
                     /locus_tag="ACF2SP_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFD2360160.1"
                     /translation="MFTRLIAWSLHNRLLILALTIILCLLGGYTLKQMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLITYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYLNRQLVNERIQQVVNRLPPGISSPVMLPVTSAVGWLLKYALVSHTASPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVQLDPKRMLAYGITVEEVRQGLATGNQNVPG
                     AFVHQGGTEFVVSTIGRIKTLDDIKKTLIVVRKGVPVTVNNVATVAFGGEIKRGDASF
                     NAEHAVIGTVSKIYGADTLTTTLKVEQALADIKKSLPAGIELNTEVFRQANFIESAIH
                     NLTKALIEGAVIVIAVLFLFLMNWRASFITFLSMPVSFIVGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRLAENPLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVLGSLVVSITMVPALCYLLLARKTEKSQDA
                     KVAVHALSEDERYHCTQEETVKQAQTETRFVIWLKKHFLTGLNWSLAHCKTVVAISIA
                     AFVSALSLLPFFGTSFLPEFHEGNFIIVMSTLPGTSLNESMRLGKQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVHLNFGKDKTMSPDELLQSIRADLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGESVESILKTVPGVVDINKEQQIN
                     VPQLVIKIDREKAARYGVNIGQISEDVQILLNGVTVSSILEGQRTFDLYLRMGETERD
                     SVKAIQNMLIDAHGAANNQETKIPLRAVADIQLEPQPFAINRENVQRVLVIGFNVQGK
                     DLGSVIQEVQHQIKEKISLPGGYFVQYGGQFESQQQASRVIVLFGALAVFIMLVLLHK
                     AFGTFREALLVMFNLPLALIGGVVSLFIASGDMSVAAMIGFITLFGIAARNGIILVSH
                     YNQLRSLGREREQVVIQGTLDRLVPVLMTAATAALALIPLLWGSPAGKELQRPLAQVL
                     LGGLLTSTVLNMLVVPTVYNAFEIWHEKRRQLKKIS"
     gene            complement(6247..7503)
                     /locus_tag="ACF2SP_002387"
     CDS             complement(6247..7503)
                     /locus_tag="ACF2SP_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360161.1"
                     /translation="MENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHL
                     GLIATFSVFALSYFFRPLGALFFGYLGDKMGRRVALQSSIMLMGLSSLCIGCLPAYDS
                     IGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGG
                     LMSTLIYLPHMPEWAWRIPFMLGTLSCFCAMYFRKNLSESPSYLTLSKEKSSNPLKIL
                     FENYKKQLIRAVLLIAALGIYIYIMNVYYASHLAKYTTLSEAQTKFIVTLGQGLVVLF
                     IVLTGMYADRIDERHIIKTGLWGYFIAAPVAFLIPQTNSFALILLSQIPYALCNALVG
                     TPIFKLLNDFFPTQIRYSGVSVAWGVSMAIFGGTAPLVANYLQYSFKLATAPILYILL
                     SASIALFILHDKKSYKITSIKLRSDN"
     gene            7726..>8164
                     /locus_tag="ACF2SP_002388"
     CDS             7726..>8164
                     /locus_tag="ACF2SP_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HFD2360162.1"
                     /translation="MQKAKAKIQNINEIMFSDRVMLLNKNLLVSIPANFLCALIIYID
                     FNKTTTDQEMLSVWFMAGVTVFVLHGGLFLFNYYRPLPSKYLLKWLISVTAIYGALWG
                     IAGSVLIPQNDLLNQMIVIIIIIGIASGGLHILQPSFLASLLFF"
BASE COUNT         2427 a         1517 c         1721 g         2499 t
ORIGIN      
        1 ctaccgcctg ctctaattcc atcgtttcca ccattaagct attgaaacag ttggataata
       61 aaaggagttt taacccggag caatctctta agaatttctg tggatataaa gaaattatga
      121 ttcaattgtt attaaaacca tttgaaacac cagtggctga aatggctagt gtacctgcaa
      181 caacttctga tttaattgaa aagcaatctg gcatcaaaac tagacttcaa gaaatgaaat
      241 cttctgcaaa tcaagataag caacctatat ttaccccttc attgatgcga ggcccaagct
      301 aaaaaacgtc actgaagtgt tagaacagtc tgtcgtctat tattttactg ctcttgtgta
      361 attacatttg ctgggagata ctaacaggac ttacgaaaaa tcccaatctc tatgctaaaa
      421 aatgttttta attcggtcat ttagtttatc taaactcctt gattaaaaac attttctgcc
      481 tcgaccttgg gttttttcgt aagtcttgaa taaatcatac aacaataaaa agaaggtatt
      541 aattgatgac ttactttcga ataaaacaca gtccattact cttgatgttt gcacctgcca
      601 cagattattt tttcgattcc atgttgactt aagatagata gattttttga gctatcgttt
      661 atggaatatc atcgtgtcag gcagttttct tatggattag tcctgctact gctgtatgcc
      721 tctctggcaa cagcggggaa cgctttgact cttaaagaag taacccaaat tgctttagca
      781 aacaacaagg atttaaaagc cgcccgttac aatgtatcta tcgcaaaagc ccggttgcta
      841 caagcaggtt tttggcctaa tccaagtttg aatctttcaa ataatgacga tcgtttattc
      901 actgatgaag gagagtattc gcgcagcgct ggctttagcc aggcatttcc catatcagga
      961 cgtattgcaa aacaaaagac agtggcacgt ctggatgtgt tgaaagcgat tacagaaatc
     1021 agagaagctg agcgccaact cagtgccaaa gtggctagtg ccttttatac ggcagtgatc
     1081 acagaacatc gcatacagca agtcaattac ttattacgga ttaaccggga attggttgac
     1141 gttatccata atcgatatca tgccgcagaa atttcaaaat tggatgacaa tgcggcacgc
     1201 attgagtatt tgcgtattgt tcaagaaaaa catcttttgg atagttttcg tattagccaa
     1261 tatgccacac tgaatcagtt aatgggacgg gaagctaatt catctatatc gcttaagatg
     1321 gattttgcca ctgaaaagcg actcccggca ttggataccc taaaaacaca ggcgcttaaa
     1381 aatcgccctg atcggcaatc tattgcgttc tcgatcgagc gtgccaatgc tgatcgccat
     1441 ttggcaaaag ccgagcgctt tgcagattgg acagtaggcc ttggtattca gcaggataaa
     1501 attgtggtgg aagaaggccc ccctcaacca gccgatcgta ctttgggcgt tacactgtct
     1561 gttcctctcc ctcttttaaa tcgaaatcaa gggcgaattt tagaagcaag tcatacaggt
     1621 actcaggcga tgatgtcatt acgtgccctc gatttaagta ttgcaacgga gatagccagt
     1681 aattatgagc aactcaaagc attacgtaag agtcttgagg aaacacaacg tgtttctctt
     1741 caattaacta ttgaaaatgt aaaattggcc cgtgattctt atgaaaatgg gcaaatttct
     1801 tttttaaatg tgctacaagt gcaaaagcaa caaaacgatt tgcaaaccac ttatctcaat
     1861 acgttagaaa aatttttaca agtctatgtg gccttgtgta cagctattgg tcctggaaaa
     1921 acagatgggt tttgctctta tttagagtat caaagcaacg gtgatagcaa atgagattgc
     1981 agcttaaaat tagaatgatg atcatgactg ttttttttct tggtttactt tcccccatgc
     2041 aagtgagttt tgcacacgga ggaaacatag aagtaagcga aggaggcacc aaaggtccgg
     2101 ttcatttgac tccggaacaa gccaggatgc ttgatatcaa agttgtccag gcaacccatc
     2161 gtcctatcgc acaattgctg ggtttaaatg gtcaaattca attattacct gatgctcaag
     2221 ctgatgtcag cattcgtatt agtggcagtg tgacagcgat tgatgtgaat ttgggggata
     2281 gtgtcaaggc aggacagcgc ttggctacag tgcaatcacg attggtggga aatcctccac
     2341 cgagcgttgc tgtcaatgcc ccaattgctg gtatcattga tgccaggaac atcaatttag
     2401 gtcaggctgt cgagccaaac accgttcttt ttcacatcag taatcgcgat aaattattag
     2461 tcgtagctca agtgtatgaa gaagatttag gtaaagtgaa agtagggcaa gaagtgaatg
     2521 ttcatgtctt aagttatccc aagcaaacat tcccgggcca agtcacttta attgagccca
     2581 atcttgatcc attgacccgt acagtcaatg tacgaattac tttggacaat aaggaggggc
     2641 tactgaaacc cggaatgttt gtgcgggcca atgtgatttt aactcacaat aaggcagcac
     2701 ttactgtgcc gaatgctgct cttttacaag cagataatca gcaatttgtt tttgtgcaaa
     2761 atggaggcac ttacaaacga gtcgatgtta aagtaggtgc tgtagaggat gactactcag
     2821 aaattattga tggtttagtt cctggcgacc tcgttgttac tcagggaaat ctcgagcttt
     2881 atacactttg gttgagtgga ggccgcaaac tgcaatcagg gcaagaggag catcattaaa
     2941 tgtttactcg ccttattgcc tggtctttgc ataatcgtct cttgattttg gcattaacga
     3001 ttattctttg tttgttgggt ggttatactc taaagcaaat gccggtagat gtttttcctg
     3061 aatttgcccc tcctcaagtt gtagttcaaa cccaagcgcc aggcatggca acccaagatg
     3121 tggaaacatt aatcacttat cctttggaaa gcgccatcaa tgggactcca ggggtagcca
     3181 gtgttcgctc aaaaacctca gtcggattat caacaattac cgtagtattt gatgataaaa
     3241 cggatatcta tctcaatcgt caactggtta atgagcgcat tcaacaagta gtcaaccgtc
     3301 ttcctccagg tatttcctca cccgtgatgc tgccagtcac atcggcagta gggtggttgc
     3361 ttaaatatgc tttggtgagt cacacagcaa gccctgaaac cttgcgcact atttctgatt
     3421 ggactattcg tccacgtcta ttggctttag gcggagtcgc ttcagtggtt tcactgggtg
     3481 gagaagtaaa gcaatatcaa gtacagcttg atcccaagcg catgctggct tatggcatta
     3541 cagtcgaaga agttcgtcaa ggattagcaa caggaaatca aaatgtacct ggtgctttcg
     3601 ttcatcaggg gggtacggaa tttgtcgtaa gtaccattgg tcgtattaag acgttggacg
     3661 atataaaaaa aacactgatt gtagtccgca agggagttcc tgttactgtt aataatgtag
     3721 ccacagtcgc ttttggcggt gaaatcaaac gcggcgatgc ttctttcaat gcagaacatg
     3781 ctgtgattgg tactgtttcc aaaatttatg gcgcagacac attaaccaca acacttaaag
     3841 tggagcaagc tttagctgat ataaaaaaat ctttgccggc aggtattgaa ttaaatacgg
     3901 aagtatttcg ccaagccaat ttcattgaat cagcgattca taatttaacc aaagctctta
     3961 ttgagggtgc tgttattgtg atcgctgtat tgtttctttt tttgatgaat tggcgagcct
     4021 cgtttattac tttcctctct atgcccgttt cttttatcgt agggatcttg gttcttcatt
     4081 attttggttt tggtattaac tccatgactt tgggaggaat ggcgattgct attggtgagg
     4141 tggtggatga tgggattatt acagtagaaa atgtggtaca tcgtttacga cttaatcgtt
     4201 tggcagagaa ccccttgcct gcgattgaaa ttgtttttga tgcggttttg gaaattcgca
     4261 gttcagtggt ttatgcgacc attatcatta gcctggtgtt tttacccatc ttctttttgt
     4321 caggaattcc cgagcggatt tttagtccat tagccattgc ttatattgcc tccgtattag
     4381 gttctcttgt cgtttctatt actatggtac cagccctatg ttatctcctt ttagcgcgaa
     4441 aaactgaaaa gagtcaggat gctaaagtgg ctgtgcatgc cttatctgaa gacgagcgtt
     4501 atcattgcac gcaagaggaa acggttaagc aggctcaaac ggaaacgcgc tttgtgattt
     4561 ggcttaaaaa gcatttttta actggtttga attggtcact ggcccattgt aaaacagtcg
     4621 tagccatctc aatcgcagcc tttgtttctg ctttaagttt acttcctttt tttggaactt
     4681 cttttttgcc tgaatttcat gaaggcaact ttattattgt gatgagtact cttccaggaa
     4741 cttcattgaa tgaatccatg cgcttgggga agcaagtcag taagactttg ttgaaatatc
     4801 cgcaagtgat ttctattgcc caaagagcag ggcgcagtga attggatgaa gatgctttac
     4861 caccgaatat tagtgaattc gatgtacatc tgaattttgg taaagataaa accatgtctc
     4921 ctgatgaatt gttacaatct attcgagctg atttagcgaa tattccagga gcattattta
     4981 atgtggggca gtttattgct catcgcatgg atgaagtgtt atcaggagtc agagcgcaag
     5041 tagccgtgaa actctttggc gataatcttg ctacccttaa cgaattgggt gaatcagtag
     5101 aatccatttt aaaaaccgtc ccgggtgtag tagatattaa taaagaacag caaatcaatg
     5161 tccctcagct ggtcattaaa attgataggg agaaagcagc tcgttatgga gttaacattg
     5221 gtcaaatttc cgaggacgtt caaatccttc ttaatggtgt gacggtatcc agtattcttg
     5281 aaggccagag gacgtttgat ttgtatttgc gtatgggtga aacagagcgg gatagtgtta
     5341 aagccattca aaatatgctg attgatgccc atggtgcggc caataatcaa gaaacaaaaa
     5401 tccctttgcg tgcggtggct gatattcagt tagagcctca gccgtttgca attaatcgag
     5461 aaaacgtgca acgtgttttg gttatcggtt ttaatgtgca agggaaagat ttgggtagtg
     5521 tgattcaaga agtacaacat cagattaaag aaaagatttc gttacctggt ggttatttcg
     5581 ttcaatatgg cggccagttt gaaagtcaac aacaagcatc tcgtgtgatt gttctttttg
     5641 gtgcgctggc agtttttatt atgttggtac ttcttcataa ggctttcggc acatttaggg
     5701 aagctctatt ggttatgttt aacttaccct tggcattgat tggtggagtc gtttctttat
     5761 tcattgccag cggtgacatg agtgtcgctg ccatgattgg ttttattact ctgtttggga
     5821 ttgcagcacg taatgggatt attttagtga gccactataa tcaattgcgc tctctggggc
     5881 gagaacgtga acaagtggtt atccagggaa cccttgatcg tctggtaccc gtgttaatga
     5941 cagcagcgac agcggctctg gctttaatac ccctattgtg gggttcccct gcaggtaaag
     6001 aacttcagcg ccctttggcg caggtgctat tagggggatt attaacttct acggttttaa
     6061 atatgcttgt agtacctaca gtttataatg cttttgaaat ttggcacgaa aagagaagac
     6121 aattgaaaaa aatttcttaa tcagagccat gatgactctg acaatggggg ctattaagaa
     6181 gcaattgtta caaattgatt agatgcttgc gtagacatgt ttttgcaatg gtgataagta
     6241 gtttgttcaa ttatctgatc ttaattttat ggaggttatc ttgtaactct ttttatcatg
     6301 caaaatgaac aaggcaatac tggcagataa aagtatatag agtattggcg cagttgctaa
     6361 tttgaatgaa tattgtaaat aattggcaac caatggtgct gttcctccaa aaatagccat
     6421 gcttactccc catgcaacag aaacaccgct atatcgaatc tgagttggaa aaaaatcatt
     6481 caatagttta aaaattggtg tgccaaccag agcattgcat aatgcatagg gaatctgtga
     6541 taagagaatt aatgcaaaag aattggtttg agggatcaga aaagcaacag gagccgcaat
     6601 aaaatacccc cacaaaccgg tttttataat atgtctttcg tcaattcgat cagcatacat
     6661 tcctgttaaa acaatgaata gtacaaccaa tccttgccct aaggtaacaa tgaatttggt
     6721 ttgagcctct gacaatgtag tgtattttgc caaatgagaa gcataataaa cattcataat
     6781 gtagatataa atacctaatg cagctattaa taatacagct ctaattaatt gttttttata
     6841 attttcaaat aaaattttca gtggattgga tgatttttct ttacttagtg taaggtagga
     6901 cggtgattca ctcagatttt tacgaaaata catcgcacaa aagcaggata aagttcctaa
     6961 catgaaaggg atacgccatg cccactcagg catgtgaggt agataaatta aagtagacat
     7021 caatccgcca aacatcatac ccaatgcgga agcaaatcca gtccagctac cagcctggca
     7081 tggtttttga gaatcatgtt caatcagata gatcgcgctg ccattaaatt caccagacac
     7141 ggcaaagcct tgaacacaac ggaatatcaa taaaagaatt ggagccaaga taccaatact
     7201 atcgtaagca ggaagacaac caatgcatag tgaagataac cccatcagca taatagaaga
     7261 ttgcagggca actcgacgcc ccatcttgtc tccaagatat ccaaaaaaga gggctcctaa
     7321 aggacggaaa aagtaactga gcgcgaaaac agaaaaggtt gctatcaaac caagatgatt
     7381 tcccaatttt ggaaaaaacg atcccgagat aatatttgcg atataggcgt aataagtaaa
     7441 ctcagcccat tccagaaaag ttccggcggt tgcaacaacg actcttttct tcttattttc
     7501 catacaataa atcaatagaa tattaaagtg atgtagagag attttacctt aaattgatct
     7561 cattattcta tgtaattgga tgtataacag agtcatagag ttactatagt gtcacattct
     7621 gctactaaca attaagtatt tttgtactat attaatacaa tatattaata caatagggtt
     7681 caaattgagg ttttgaattt gtcactttat ctcagtgagt gctgaatgca aaaggccaaa
     7741 gcaaagattc aaaatataaa tgaaataatg ttttcagatc gcgtaatgtt actaaataaa
     7801 aatttattag tgagcattcc tgcaaatttt ttatgtgcat taattattta tattgatttc
     7861 aataaaacaa caacagatca agaaatgctc tcagtatggt ttatggctgg agtaacagta
     7921 tttgttctcc atggaggttt atttttgttt aattattatc gtcccttgcc atccaaatac
     7981 cttttaaaat ggttaataag cgtcactgcg atttatgggg ctctatgggg aatagcaggc
     8041 tcggttctta ttcctcagaa tgatctgtta aatcaaatga ttgtcataat tataattatt
     8101 ggaatcgcat caggtggatt acatattctt cagcccagct ttttagcaag tctattattt
     8161 ttta
//
LOCUS       DAXLDT010000132         8105 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXLDT010000132 DAXLDT010000000
VERSION     DAXLDT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.132"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(138..1460)
                     /gene="bioA"
                     /locus_tag="ACF2SP_002389"
     CDS             complement(138..1460)
                     /gene="bioA"
                     /locus_tag="ACF2SP_002389"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFD2360163.1"
                     /translation="MVNTDQLIAKDLKHIWHPCSQMKDFETFPPIVVNKAQGSYLYTN
                     KGPVIDAISSWWCKSLGHGHPAVIAAIKNQLDCFEHVISANTTHPAAVELAEKLSEIT
                     GKQHVFFASDGSSAVEIAMKLAIHASQIKGHKNKNQFIALKNGYHGETIGTMSVSDLG
                     LYKTPYASFGVQCHFIDDIPYVSGLKDPLWTNCDAYWNNVVQQLNKICDKVCAILLEP
                     IIQGAGGMLCYSADFLSKISSWAKEKDIYLIADEIMTGIGRTGEWLACDHARVQPDLI
                     CLSKGLTSGSIPFSCVMIDSTLFDLFYDDYHTGKSFLHSHTYSGNPLAIGAALATIKT
                     IEQDNILMHSQELGKIMFSYLQDIARDSRKLVNVRGVGAVVAADLIESGQARIGFKIY
                     QEAIKQGALIRPIGNTLYWLPPLNTDKEIIGKLAEITLNSIEKAYNKA"
     gene            1533..2480
                     /gene="bioB"
                     /locus_tag="ACF2SP_002390"
     CDS             1533..2480
                     /gene="bioB"
                     /locus_tag="ACF2SP_002390"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFD2360164.1"
                     /translation="MSEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFA
                     TLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAA
                     WRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
                     YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPE
                     SVPINRLIPVEGTPLAQAERVEGIELVRTIATARILMPKSAIRLTAGRTEMSDELQAL
                     CYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV"
     gene            2481..3623
                     /locus_tag="ACF2SP_002391"
     CDS             2481..3623
                     /locus_tag="ACF2SP_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFD2360165.1"
                     /translation="MTLNIKINEYTAQLQEQGLLRTRQILETGKIDYIRFDSNDYLSL
                     TEDRRIAEAYQKGYELYPCGSGASMVLSGYHSSHQTLERAFAEMLGVDDCLLFSSGYC
                     ANLAITALLGRLGTHCFIDKSVHASIYDGLALSRVTYSRYIHNDMNALSALLKSHLGD
                     SVLITEGIFSMSGQIAPLSTISTLCKENGSELFVDEAHAFGVIGPQGMGSVPGHGLTQ
                     NEVPLRVIPFGKAFASQGAVVGGRKDWINALLQTARSFVYSTAISPALCYGLLKTLEI
                     IAAADSRREKLNQLVAQFKELIKSSPLNWLDSDTPIQQLRLGCPHKALHYASELKKDG
                     IYCSAIRAPTVNSASSGLRIIINYKHTIEQIIELFNKLHLIHEYSS"
     gene            3607..4326
                     /locus_tag="ACF2SP_002392"
     CDS             3607..4326
                     /locus_tag="ACF2SP_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD2360166.1"
                     /translation="MNIHLDKHGQGMPLVLFHGWGFDNQIWQPIIPYLKPKYQIILVD
                     LPGFGLTPMMDWESFKKNLLDQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITS
                     SPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
                     LAFGLEILGTWDFREQLKQITIPTVYLFGRLDPITPVKTMAIMEKNYPNFKYVLFNRA
                     AHMPFLSHTDLFITMMDEFIK"
     gene            4323..4961
                     /gene="bioD"
                     /locus_tag="ACF2SP_002393"
     CDS             4323..4961
                     /gene="bioD"
                     /locus_tag="ACF2SP_002393"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFD2360167.1"
                     /translation="MKRYFVTGTDTDCGKTFVTNQLVNYFSNSAAIKPIASGCEYSEN
                     QLVNSDALLHQQQNHLSLEIINPWRFRLPVSPHLSAREDGASIDVHKVADYCLNLQLN
                     DIKKLFIEGAGGLMVPLNEQDTWLDFLKLTRIPVILVVGMKLGCINHTLLTQEVLEIN
                     KIKCQGWIANCLDQDMLMLDENINTLEAKLKYPLLARTNYGGKISDICLSSL"
     gene            5096..5410
                     /locus_tag="ACF2SP_002394"
     CDS             5096..5410
                     /locus_tag="ACF2SP_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HFD2360168.1"
                     /translation="MPIYEYQCTNCHHQFDLMQKVTDAPVKQCPKCYENTVTKLISPA
                     GFQLKGTGWYVTDFKNKGNGPKESTENKSGDPGINKENSSKETPAASSADSAKSTKGD
                     SE"
     gene            5407..6036
                     /locus_tag="ACF2SP_002395"
     CDS             5407..6036
                     /locus_tag="ACF2SP_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFD2360169.1"
                     /translation="MKLISLRAYLITGLIVWLPIFVTIVVLRFIIDMLDSTLALFPKA
                     YQPEQLFGFYIPGFGVLFSLVLLLVTGIIATNFLGQRLVSRGESLLAKIPLVRSIYNA
                     VKQVIHAVLSTNSQAFRKVVLVEYPRKGLWTIAFQTGSVNPEIKEKSKEDMMSVFVPT
                     TPNPTSGFMLMIPRQDAIELNMSIDEALKLVISLGVMQSDPALAQVKTQ"
     gene            6055..7836
                     /gene="aspS"
                     /locus_tag="ACF2SP_002396"
     CDS             6055..7836
                     /gene="aspS"
                     /locus_tag="ACF2SP_002396"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFD2360170.1"
                     /translation="MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGL
                     LQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGIQLEIL
                     NQSPTPPFLPDDHQIINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
                     DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCF
                     RDEDLRADRQPEFTQLDIEMAFINEEDILQLIEGLLKVVFKEILNITLPDKLPRMSYK
                     EAMTRYGSDKPDLRNPLELIDIADLVKDCDFNVFASAANDNSGRVVALKLPNGCDLSR
                     KDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEAQTG
                     DVIFFGADKAHVVNESMGALRNKLGHDRNLINPGWQLLWVIDWPMFELDPQSNKLQPM
                     HHPFTSPQELSAEALRSKPTQTLAKAYDIVINGYEIGGGSIRIHQPELQKTVFDLIGI
                     DEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKTQTASCP
                     LTSAPSPAGNAQLTELGIRLAPTITTK"
     gene            7811..>8105
                     /locus_tag="ACF2SP_002397"
     CDS             7811..>8105
                     /locus_tag="ACF2SP_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360171.1"
                     /translation="MRQLLRQSNLNLSTTSFKYLWRFFLPLFLLLFSFSLFSSSVKEN
                     NATLVEYLAHEKTHLTIAINEIKQKSAPKDEQEYNEKLRQIRTILSMNKAKIES"
BASE COUNT         2477 a         1416 c         1685 g         2527 t
ORIGIN      
        1 gattcaaacc aaagaagagt taggttagca ggttttaggc aaattacttg gttttgggcc
       61 aaggacattg ctattctttg gacaattggc gaacagattt gtctttaaac tatttgtgaa
      121 taaccttact catatcctta agctttgtta taggcttttt ctatagagtt taatgtgatt
      181 tctgctaatt tcccaatgat ttctttatct gtgttaagag gtggtaacca atagagagta
      241 tttccaattg ggcgaatcag tgcgccttgt tttattgctt cctgataaat tttaaatcca
      301 attcttgctt gaccgctctc tatgagatca gccgcaacaa ctgctccaac accacgaaca
      361 tttacaagtt ttctactgtc ccttgctata tcctgcagat aggaaaacat gattttacca
      421 agttcctgac tatgcatcag tatattatct tgttctatgg ttttaatagt tgccaaagca
      481 gcgcctatag caagtggatt accactatag gtatgtgagt gcaaaaaaga tttccctgta
      541 tggtaatcat cataaaaaag atcaaataag gtactgtcaa tcatgacaca gctaaatggt
      601 attgagccgg aagttaatcc tttagataga caaattagat caggttgtac ccttgcatga
      661 tcacaggcta gccattctcc agttctgcct ataccagtca taatctcatc agcaattaaa
      721 taaatgtctt tttccttagc ccatgacgag atttttgaga gaaaatctgc actgtaacat
      781 agcatacctc cagcaccctg aatgataggc tccagaagta tcgcgcaaac tttatcgcaa
      841 attttattca attgttgtac gacgttattc caataagcat cacaatttgt ccataaggga
      901 tccttaaggc ctgagacata gggaatatca tcgatgaaat ggcattgcac accaaacgat
      961 gcatagggag tcttatagag tcctaaatca ctgacactca tggtgccaat agtttctccg
     1021 tggtagccat tttttaaggc aatgaattgg tttttatttt tgtgcccttt tatttgtgaa
     1081 gcatgaatgg ctaatttcat ggcaatttca acagcactgg aaccatcact ggcaaaaaac
     1141 acatgctgtt ttcctgtaat ttcacttaat ttttcagcta attccactgc agcagggtga
     1201 gttgtattgg cactaatcac atgctcaaaa cagtccagtt ggtttttgat agcagctatc
     1261 actgctggat gaccatgccc taatgatttg caccaccaac tggaaattgc atcaatcacg
     1321 gggcccttat tggtatagag atagctacct tgtgctttgt tgacaacgat aggggggaat
     1381 gtttcgaaat ctttcatttg tgaacaagga tgccaaatgt gctttaagtc ttttgcaata
     1441 agttgatctg tgtttaccat tttaaaaatt ttggttgaca agtataggcg cgactttatc
     1501 atgttcacta ttctgaatta aagggggtag ccgtgtctga agaaaaaaaa cagtggtcaa
     1561 tttctgatat tgaagcaatt tatcaacaac catttaatga tttgctttat caagcacata
     1621 ccattcatag gacatatcac gatcctaatt ctttacaatt tgcaaccttg ctaagcatta
     1681 aaacgggcgc atgcccagaa gattgtggct attgctctca aagtggtcac tataaaacgc
     1741 atgtagaaaa agaaaaattg atgtcagttg aagaggtatt gcaatgtgcc aaagaagcaa
     1801 aggagggtgg ggccaaacgt ttttgtatgg gagctgcatg gcgttgtcca cctgataaag
     1861 caattccgca gctaaaagaa atgatagaag gagtaaaatc tttagggctt gaaacctgta
     1921 tgactttagg catgcttacc aaagaacagg cctctcattt aaaggaggct ggattggatt
     1981 attataacca taatattgat acctcaccat cctattacga taaggtggta accactcgaa
     2041 aattcagtga tcgattagat actttaaata acgttcgttc agcgggaatt aatgtgtgtt
     2101 gtggtggtat tttaggattg ggggaaacca gagaagatcg aatcgaattt ttattaacat
     2161 tagctaatat ggaaacccct ccggaaagcg tgccaattaa tcgtttaatt ccagtggaag
     2221 gaacaccttt agcccaagct gagagagtag aagggattga attggtaaga accattgcta
     2281 cagcaagaat tttaatgcct aaaagtgcta ttagattgac agctggtcga accgaaatga
     2341 gcgacgagtt acaagcactt tgctattttg ccggagccaa ttctgtgttt attggcgata
     2401 aattattgac agaagataat ccccagcgct tcaaagacaa aactcttttt aacaaactgg
     2461 gtttgactga gatggtataa atgactctta atataaaaat taatgagtat acagcacagc
     2521 tgcaagaaca aggattattg cgtacaagac aaattttaga aacgggtaag atagattata
     2581 ttcgatttga ttctaatgat tatttgtcgc tcacagaaga taggcgaatt gctgaagcct
     2641 atcaaaaggg atatgagttg tatccttgtg gaagtggtgc ttcgatggtt ttaagtggct
     2701 accatagtag ccaccaaacc cttgagaggg cctttgctga aatgctggga gtagatgatt
     2761 gtcttttatt ttcctctggc tactgtgcaa atttggccat tacggccttg cttgggcgtt
     2821 tgggaacaca ttgctttata gataaaagcg ttcatgcgtc aatttatgat ggattggctt
     2881 tgtcacgagt aacctactct cgttacatac ataatgacat gaatgctttg tctgccttgt
     2941 taaaatctca tttgggcgat tctgttttga ttacagaagg tatttttagt atgagtgggc
     3001 agatagcgcc gttatcaacg attagtacgt tatgcaaaga gaatggctct gagctctttg
     3061 tagatgaagc gcatgctttt ggtgtgattg gaccgcaagg aatgggctct gtgccaggcc
     3121 atgggctgac acagaatgaa gttcccttgc gagttattcc ttttggtaag gcatttgctt
     3181 cccagggcgc agtggttggt gggcggaaag attggataaa tgctttgttg caaacggcac
     3241 gttcttttgt ttattcaact gctattagtc ctgctttatg ctatggtttg ctcaaaacat
     3301 tggagataat agcagctgcc gactccagaa gagaaaaatt aaaccaatta gttgctcaat
     3361 tcaaagaatt aataaagtct tcgcccttaa attggctgga ttcagatact ccaattcagc
     3421 aattaaggtt aggatgccct cataaggcat tgcattatgc cagtgaactg aaaaaagatg
     3481 ggatttattg ctctgctatc agagctccaa cggtaaatag tgcttcttcc ggtttacgga
     3541 ttattattaa ttataagcat actattgaac aaatcattga gttatttaat aaattgcatt
     3601 taatacatga atattcatct tgataaacat gggcaaggta tgcctttggt tctatttcat
     3661 ggctggggat ttgataacca aatctggcaa ccgataattc cttatcttaa gcctaaatat
     3721 cagattattt tggtcgattt gcctggtttt ggattgactc caatgatgga ctgggagagt
     3781 tttaaaaaga atttgcttga ccaattacct gataaatttg cattggcagg gtggtcaatg
     3841 ggtgggctct acgcgacacg tttagcaatt gaagagcctg ccagagtgca gtatttgata
     3901 aatataacgt cttcacctcg ctttatctct gatattgatt ggccgggagt agcagaggag
     3961 gtatttgtca atttttataa caatttatcg aaagatatta ataagacatt aaaagagttc
     4021 atatctttgc aactcaataa gatgaaattt gattttaaaa taggtaatcc tccatctcca
     4081 gaaggcttgg cgtttggtct cgaaattttg ggtacctggg attttagaga gcagttgaag
     4141 caaataacta tacctactgt ttacttattt ggtcgacttg atccgattac tcctgtcaag
     4201 accatggcaa ttatggagaa aaattacccg aattttaaat acgtcctctt caacagagca
     4261 gctcacatgc cttttctgtc ccataccgat ttatttatta caatgatgga tgaatttata
     4321 aaatgaaacg atattttgtt acgggtactg acactgattg tggtaagaca tttgtaacca
     4381 atcaattggt taattatttt tcaaattcag cagcgataaa acccatagca agcggatgtg
     4441 agtacagtga aaatcaatta gtaaattctg atgcactttt acaccagcaa cagaatcatt
     4501 tgtctttgga aattattaat ccctggcgat ttagattgcc agtttcgccg cacttatcag
     4561 caagggagga tggtgcaagt attgatgtcc ataaagtggc tgattattgc cttaatttgc
     4621 aactgaatga tattaagaaa ttatttatcg aaggagccgg gggattaatg gttcctctga
     4681 atgagcagga tacttggctt gattttttaa aattaaccag gatccctgtt attttagttg
     4741 tgggcatgaa gttgggttgc atcaaccaca ctttgcttac ccaggaagtg ctcgaaataa
     4801 ataaaatcaa atgtcaagga tggattgcta attgcctgga tcaggacatg ctaatgcttg
     4861 atgaaaatat taatactctt gaagccaaat taaaataccc tttattagcc aggacaaact
     4921 atggcggcaa gatttctgat atatgtttaa gctccttgta attgcagtaa aattttatat
     4981 caaaacagca ctatatacct gatgttattg tttgttctgc ttgcgctata cccatggcag
     5041 caattataat gaaaagttaa attacagata cttcgagttt atggagtgat gctagatgcc
     5101 aatttatgaa tatcagtgca caaactgtca tcatcaattt gatttgatgc aaaaagttac
     5161 ggatgcgcct gtaaaacaat gcccaaagtg ttatgaaaat actgtaacaa aacttatttc
     5221 tccggctgga tttcagttaa aggggactgg ctggtatgtc acagatttta aaaacaaagg
     5281 aaatggacca aaggagtcaa ctgagaataa atcaggagat cccggcatca ataaagagaa
     5341 ttcttctaaa gaaactcccg cagcaagctc tgcagatagc gcaaaatcga caaaaggtga
     5401 tagcgagtga aattaatctc cctcagagcc tatttaatta ccgggttaat agtgtggtta
     5461 ccaatatttg taaccattgt agtcctgcga tttattattg atatgcttga tagtaccttg
     5521 gctttatttc caaaagctta tcaacctgag cagttattcg gtttctatat accaggcttt
     5581 ggcgttttat tttcattggt tttattactc gtgacaggaa ttattgcaac caattttttg
     5641 gggcagagac ttgtaagtcg gggtgaatcc ttattagcta aaatacctct ggtgcggtca
     5701 atttataatg cagtaaagca agttattcat gctgttctat caactaatag ccaagctttt
     5761 cgtaaagttg ttttagttga atatccccgt aaaggacttt ggactatcgc atttcagact
     5821 ggctccgtta atcctgaaat taaagagaaa tccaaggaag acatgatgtc tgtatttgtt
     5881 ccaactacac ctaaccctac ttcgggtttt atgttgatga tacccaggca agatgccatc
     5941 gagttgaata tgagcattga tgaagcgtta aaattagtaa tatcgttagg cgtaatgcag
     6001 tctgatcctg cattggcaca agtaaaaact caataaactt taataggtgg ttttatgcgt
     6061 acacactact gttcggccgt gaatgaatta agcttggata agcaaatcac agtttgtggg
     6121 tgggtgcata atagacgaga tcatggcggg gttatctttt tagatattcg tgatcgttca
     6181 ggattattgc aagtggtgta cgagcctgaa aataaggaaa tttttgcaat tgctgaaaaa
     6241 ctaagatcag agtttgttgt tcgtgttact ggcatagtgc gtaaaagacc agaaggaatg
     6301 atcaatgata aaatggagac tggccgggtt gaggtaatcg gtatacaact tgaaatcctt
     6361 aatcaatctc caactccacc gtttttgcca gatgatcatc aaattatcaa tgaagatttg
     6421 cgctataaat accgctatat tgatttgcgg cgcgctgtta tgcagaagaa actgacttta
     6481 agacacaagt taaacagttg catacgtaat tacttgaatg agcagaactt tttagatata
     6541 gaaacgccta tgttaactaa agcaactccc gaaggcgcac gggattatct tgtgccttca
     6601 agggtgcatc cagggcagtt ctatgcttta cctcaatcgc cacaattatt taagcaattg
     6661 ttgatgatgt caggatttga taaatattac cagattgtac gctgttttag agacgaagat
     6721 ttaagagctg acagacagcc tgaatttacg cagcttgata tagaaatggc atttattaat
     6781 gaagaagata tattgcagct aattgaagga ttgcttaagg ttgtatttaa ggaaatcctt
     6841 aacattacct tacctgataa attgccaaga atgtcgtata aagaggctat gacacgctat
     6901 ggaagtgata aacctgatct gcgtaatcca ctggagttga ttgatattgc agatttggta
     6961 aaggattgcg actttaatgt atttgcctct gctgcgaatg ataattcagg aagagtggtt
     7021 gctttaaaat tacctaatgg atgtgatctt agtcgaaagg atctggataa ctatggacaa
     7081 ttcgtaacta tatatggggc taaaggactt gcatatatta aagtcaacga tttaagtgct
     7141 ggtatggcgg gtttgcagtc accaattcta aagtttttat ctgaaacggc tgtgcaatct
     7201 attcttaatc gggttgaggc gcagactggc gacgttattt ttttcggtgc tgacaaagct
     7261 catgtagtaa atgaatctat gggagcctta aggaataaac tgggtcatga tagaaatctg
     7321 attaatcctg gatggcaatt actatgggtt atagattggc ctatgtttga gttggatcct
     7381 cagagcaata agttacagcc tatgcatcat ccctttacat cccctcagga attatctgct
     7441 gaggcacttc gctccaagcc aacgcaaaca ttagccaagg catatgatat agttattaat
     7501 ggttatgaaa ttggcggtgg atctattcgt atccatcaac ctgaactaca aaaaactgta
     7561 tttgatttga tcggaattga tgagcaagaa gcacatgaga aatttgggtt tttactcgat
     7621 gccttacaat atggtgctcc acctcatgga ggaattgcgt tgggaattga tagattagct
     7681 atgctgctga cagactcaac ttccataagg gatgtgattg cgtttcccaa aactcaaaca
     7741 gcatcttgtc ctttaacaag tgcgccatcg cctgcgggca atgcacaatt aactgaactg
     7801 ggtataagac ttgcgccaac tattacgaca aagtaattta aatttaagca ccacaagttt
     7861 taaatacttg tggcgctttt tcctgcctct atttttatta ttatttagtt tttcgctctt
     7921 ttcatcgtcg gttaaagaaa ataatgccac gctagtagaa tatttggctc atgaaaaaac
     7981 tcatttgacc attgctatca atgaaattaa gcaaaagagc gctcccaagg atgagcaaga
     8041 gtacaatgag aagttaagac aaattagaac tattctttca atgaataaag caaaaattga
     8101 gagtt
//
LOCUS       DAXLDT010000133         8013 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXLDT010000133 DAXLDT010000000
VERSION     DAXLDT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.133"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(68..1264)
                     /locus_tag="ACF2SP_002398"
     CDS             complement(68..1264)
                     /locus_tag="ACF2SP_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD2360172.1"
                     /translation="MINSKLIGGILLIVGTSIGGGMLALPVSTAEVGFTNSLFFLFFC
                     WLVMTAGALLILEVNLRLPAGSNMISMAKSTLGLPGQVIAWITYLLLLYTLLAAYISG
                     GSDVFSSLLHKAHIDLPNWITSVIFTAMFSLIVYSGIRSVDYVNRGLMFGKLGVYILL
                     VIIISPHVSTAGLTGGSAKAFTGTLMILVTSFGFASIVPSLRDYFNDDIRSLRRVILF
                     GSLIPLFCYIVWDAVIMGVISREGENGLLSLMNSDHATSGLTEALSQSVSSQWISGFF
                     GFFTSICMVTAFLGVSLGLFDFLADGLKLKKSGNQGKYTLALTFIPPLAVVLFNPGIY
                     LHALSYAGVCCVILLLLLPSIMAWKGRQTCSQSPSETHLIPGGNPALSLIGFIAIGLL
                     IIAIMY"
     gene            complement(1465..2655)
                     /locus_tag="ACF2SP_002399"
     CDS             complement(1465..2655)
                     /locus_tag="ACF2SP_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD2360173.1"
                     /translation="MRSRFIGGILLIVGTSIGGGMLALPVANAATGFWQSSLFLLICW
                     AFMTFGAFFILEANLYLSRGKHMVSMAKATLGDYGLLTAWISYLFLLYTLLSAYISGG
                     ADVLNSLLSKVNWHLSDWQASSLFTLIFGLVVYGGIHSVDVANRALMFGKLAVYCILV
                     ILIAPHIEFHYFQHGDYRQIGGAIMILITSFGFAIIVPNLRDYFDDDIKTLKKVIFIG
                     SLIPLICYIAWDAVIIGSLPSAGTSGLESLMQNPRTTSALASLLTDKVENTTISALFN
                     FFTSICMLTAFLGVSLCLFSFLADGLKLRERGSHGIGLFVLTFLPPLLIVIYYPGAYI
                     YALRYAGIFCIILLLLLPALMCYFGRKRYKSPFWVPGGKISQFAVIIISVVLLAHEFI
                     ALFK"
     gene            complement(2666..3358)
                     /gene="cmpA"
                     /locus_tag="ACF2SP_002400"
     CDS             complement(2666..3358)
                     /gene="cmpA"
                     /locus_tag="ACF2SP_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-OmpA-like family protein CmpA"
                     /protein_id="HFD2360174.1"
                     /translation="MSRKLAKTRILGYGLMICFLAGCFHPPYNNFQPDRRAVKRVGVD
                     TGIGAVAGVIASGTASGTLIGAAAGGTVGLVASIYRDSKRKIIRDLQKQDIQYVEYGD
                     TRTLIIPTDKYFMFSSPRLNEICYPGLNNVIRLLNFYPQSTIYVAGFTDNVGSRSHKR
                     KLSQAQAETMMTFLWANGIAAKRLKAEGYGDKNAISDNAIIHGSAQNRRIEIQWFTSP
                     AQPPQPQMAYVK"
     gene            4242..5087
                     /locus_tag="ACF2SP_002401"
     CDS             4242..5087
                     /locus_tag="ACF2SP_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFD2360175.1"
                     /translation="MSKKAIAISIVIALFFITAIWFAYSFVKQEENQNWLTLYGNIDI
                     RDVSLSFRVSGRILSMDVDEGAHVKKGQVLARLDKDTFIANLEMAKAQLEQAIANQKN
                     ASRTYKRREQLVDDGAVSKALFDDAVAMKDSSSAEVSVAKANLEKAQISLNDTEIHAP
                     THGTILTRIQEPGAIVNVAEPVYTLAIDNPVWVRTYIDEPFLGRIYPGQDALVYTDSN
                     PEKPYKGHIGFISPQAEFTPKIVETTQLRTDLVYRIRVVIDQPDNGLRQGMPVTVKIH
                     LKANS"
     gene            5090..6832
                     /locus_tag="ACF2SP_002402"
     CDS             5090..6832
                     /locus_tag="ACF2SP_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFD2360176.1"
                     /translation="MNSSKPLVSINGVSKCFPGNTDPALDNVSATIFEGQITGLVGPD
                     GAGKSTLMRLICSLMLPTQGTITVDSLNTRTDSEKIHAITGYMPQKFGLYEDLTIIEN
                     LRLYAELRNLHGEKREEQFRTLLKFTALEPFQRRLAGNLSGGMKQKLGLACALMGEPK
                     LLLLDEPSVGVDPISRRELWTMVQGLLGRGMGVVWSTAYLDEAEKCDQILLLNAGKPI
                     YHGKPGDFLQKTKDRTFQICGVSLLNRRQALMNALNTPEVIDGVIQGKNIRIVISDKK
                     IKPSLDLITREPGVHFQEVESRFEDAFIDSLKTKISGRSLLAEHIAEKPHSDKPIIEA
                     RKLTKRFGSFTAVSNNEFTIKRGEIFGLLGPNGAGKSTTFKMMCGLLQPTQGQAFVMG
                     LDLKTSSSEARSRIGYMAQKFSLYSHLDALQNMRFFSGLYGLSGKRQEAQINSMIEIF
                     DLKKHLKQNSGDLPLGFKQRLALACAVMHEPDVLFLDEPTSGVDPLTRREFWTHINGM
                     VNKGVTVMVTTHFMDEAEYCDRIALIYRGKNIATGTPDDLKDKVRNSTLPNPTLEDAF
                     IELIRLDELREPVH"
     gene            6850..7971
                     /locus_tag="ACF2SP_002403"
     CDS             6850..7971
                     /locus_tag="ACF2SP_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2360177.1"
                     /translation="MNIRYLKALVIKETLQIIRDPSTILIAFVLPLILLFIFGYGVNL
                     DTDQVKFGLALESYDSSTVSLSQSFSSSRFLDVSISTNRQAFRYGLVNGTIRGFIDIP
                     QRFTADNLSGNPKPALQTIVDGSYPQVAGFAENYALGALQTWVSSQAYEHGQMTLKSG
                     INLEPRYWYNQELKSRNFLIPGSIVIVMTLIGTLLTSLVIAREWERGTMEAMMATPIS
                     IVDIILGKLIPYFVLGMGSMFLCTIIATWYYGVPFQGTLLALTLVSAVFLVAALGQGL
                     LISTLARDQFVASQLALMSAFLPSFILSGFIFEINSMPKWIQLLTHLFAARYFVTSLQ
                     TLFLTGNIWSLLIRCLFAIALIGFAFFFITAHKTRKRLD"
BASE COUNT         2520 a         1547 c         1640 g         2306 t
ORIGIN      
        1 tactggtttc gctttacttt aaaatatatc aaatttgtac tttttaacgc tgatattcgg
       61 gctttaatta atacatgatc gcaatgataa gcaaaccaat ggcgataaat cctataaggc
      121 ttaaagctgg atttcctcca ggaatcaaat gagtttctga tggagattgg ctacaggttt
      181 gtcttccttt ccatgccatg atagaaggta ataacagcag taaaatgaca caacaaaccc
      241 cggcataact taatgcatgc aggtagatac ccgggttaaa caacaccacg gccagtggag
      301 ggataaaagt gagagccagc gtatacttgc cttgattacc agatttttta agctttaatc
      361 catcagcaag aaaatcaaat aatcctaatg aaactcccag aaatgcagta accatacaga
      421 tagaagtgaa aaacccaaaa aatccactga tccactggct gcttaccgat tgactaagtg
      481 cttcagtgag gccgctggtt gcatgatccg agttcattag ggacaataaa ccattttctc
      541 cctcgcgtga gataacgccc ataatcactg catcccagac tatgtagcag aataatggga
      601 taagagaacc aaacaaaata actcgacgta agctacgtat atcatcatta aaataatcac
      661 gtaagctggg cacgatggat gcaaatccaa aagaggtgac caagatcatg agagtgccag
      721 taaaggcttt cgctgagccg cctgttaatc ctgctgtgga tacatgggga ctgataataa
      781 taaccaataa aatataaacg cccagtttgc caaacataag tccacgatta acgtagtcta
      841 cagagcgtat accactatat acaataaggc tgaacatggc agtaaatatt acagacgtta
      901 tccaattcgg taaatcaatg tgggccttat gcagcaggct gctaaataca tcacttccac
      961 ctgaaatata agccgccagg agggtataaa gaagaagaag gtaggttatc caggcaatta
     1021 cctgacctgg caggcccaag gttgatttcg ccattgaaat catgttgctt cctgccggga
     1081 gtcgtagatt tacctcaaga attaataaag ctccagcggt catcaccaac cagcaaaaaa
     1141 acaggaaaaa taatgagtta gtaaatccca cctcggctgt agaaacaggt agcgctagca
     1201 tcccaccacc aatcgaagtg cctactatga gtaatatacc accaattaat tttgaattga
     1261 tcacgaagta taacccaaaa aacagtacaa atacagaaca aaatgatact ttgttcctca
     1321 aaaaaagtca acttccttat tgtagacctc aatcattttc ttttcgcaaa tgacctggcg
     1381 ttcaatttat acatcctgta tatcaggttt taatgtttta ttgctataaa gtaaaaacaa
     1441 ggccagtaat aacattaatc atttttattt aaaaagcgca ataaattcat gtgccaataa
     1501 gactacagaa ataataatca ctgcaaactg tgatatttta cctcctggaa cccaaaaggg
     1561 tgatttgtaa cgctttctgc caaaataaca cattagggct ggcaataata gtagtaatat
     1621 gatgcaaaat ataccggcgt agcgcagagc ataaatataa gcccctggat aataaatgac
     1681 gattagtaaa ggcggcaaaa aagtcaaaac aaataagcca attccatggc tgcctcgctc
     1741 tcgcaatttt aagccatcag caagaaaact aaataaacaa agtgatacac ctaagaaggc
     1801 agttaacatg caaatggagg taaaaaaatt aaagagggcg ctgattgtgg tattttcaac
     1861 tttatcagtt aacaaactgg ctaacgcgct ggttgtacgc ggattttgca ttagactttc
     1921 aagtccgcta gtgcctgctg aagggagtga accaataatt acggcatccc aggctatgta
     1981 acatatcaaa ggtattaatg aaccaataaa aatgactttt tttaatgtct tgatgtcatc
     2041 atcaaaataa tctctcagat ttggcacgat aatagcaaaa ccaaaagaag taattaaaat
     2101 cataattgcg ccaccaattt gtcgataatc accatgttga aaatagtgaa attcgatgtg
     2161 tggggcaatc aggataacaa gtatgcagta aacggctaat ttaccgaaca tgagagcgcg
     2221 gttagcaaca tcgacggaat ggatgccacc atataccacc aggccaaaaa ttaaagtaaa
     2281 taacgaactt gcctgccaat cactgagatg ccaattgact ttggacaata gactgttcaa
     2341 tacatcagcg ccaccggaaa tataagcgga aagcaaggta tacaataaga aaaggtaact
     2401 aatccaggca gtaagtaacc cataatcgcc cagagttgcc ttggccatgg aaaccatgtg
     2461 ttttcctctg gacaaataga gatttgcttc caggatgaaa aaggcaccaa aggtcataaa
     2521 agcccagcag ataagaagaa acagagaaga ttgccaaaaa ccggttgctg cattagctac
     2581 tggtagagcc aacatccctc caccaatgga ggtaccaact atcaataaga tgccgcctat
     2641 aaatcttgaa cgcattaata aacctttatt ttacataagc catttgtggt tgtggaggtt
     2701 gagccgggct tgtaaaccac tggatctcga ttcttctgtt ctgagcgctt ccatgaataa
     2761 tagcattgtc agaaatcgca ttcttatctc catagccttc tgcttttagt cttttggctg
     2821 caataccatt agcccagaga aaagtcatca ttgtttcagc ttgcgcttga gaaagttttc
     2881 ttttatggct gcgtgatccg acattatcag taaatccggc aacataaatc gtactttgag
     2941 gataaaaatt taacagcctg atgacattgt ttaggccagg atagcatatt tcattaaggc
     3001 gagggctgct aaacatgaaa tatttgtctg taggaataat taatgttctt gtatctccat
     3061 attcaacata ctgaatatcc tgcttttgca gatctctgat gattttgcgt ttgctgtcac
     3121 ggtagatact cgcaactaga cctacggtac caccagccgc tgccccaata agagttcctg
     3181 aagcagtacc actggctatt accccagcta cagcaccaat ccctgtatct actcctactc
     3241 tcttaacagc tcgtctatcg ggttgaaagt tattataagg gggatgaaag cagcccgcaa
     3301 gaaagcaaat cattaatcca taaccaagga ttcgtgtttt ggctaatttg cgtgacatat
     3361 ccactccttt aatcaatgat ttaatcatta ttaagcatct cattaaattt cgcaatctaa
     3421 cactttatat ttttacagga cgtataaaaa aataattttc ttcgtctata attgaaatag
     3481 tcaaggaaga aacagagtga tgcactgtga aaggaaattc agtgtgccca ctcgcccaaa
     3541 tgcaaggagc ggtatgcctg gctcattacc cggtttaccg aaaaagctat gaaaattcca
     3601 gtccaactca aaaatatagg cggtaatatc ccagaaaatg atattgcttt gcaagtttct
     3661 tattttataa atctcaaaat aacgaattgc ttccccaaag tgacgcttat attgctccag
     3721 cttcaggcaa gattgatgtc agttttggaa ataatctcgc gcgaaataaa gatgttttct
     3781 ttgctgtaat cagtactgca gacaagaaaa taattttgct gaatcttaag gatgataatt
     3841 gggtttgata tgggcatcca aaatcaggta atgcaactct atatatattt gagatacacc
     3901 ttttaggggt agagtatctg tgtaaatttg gctccattcc ctaacggccg tggctctgat
     3961 tattaatcag tatcaacttg ctctatgtcc ccgatttaaa actattttaa aataataaat
     4021 tatggcatta tgactttcta tttatgcttt attgataaga acaaaactta cgaaaaaaaa
     4081 ccaaggtcca ggcaaaaaat gtttgtaatg agggagttta gatagactaa acgaccgcat
     4141 tgaaaacatt tttaacaaag agattggagt ttttcataag tcctgaagaa aatataatgc
     4201 gaacaaaggg ttagtacgcc aaggaattca tggagaacta tatgagtaaa aaagcgatag
     4261 caataagtat cgtcatcgca ctttttttca tcacagcaat ttggtttgcg tacagctttg
     4321 ttaaacagga agaaaaccaa aattggctta ctctttacgg aaatattgat atacgtgacg
     4381 tttcattatc ctttcgtgtc tcggggcgta tcctgtctat ggacgttgat gaaggtgctc
     4441 atgttaagaa ggggcaagta ctcgccaggc ttgataaaga tacttttata gccaatctgg
     4501 aaatggcgaa agcccaattg gaacaagcca ttgccaatca aaaaaatgca agcagaactt
     4561 ataaaagacg tgagcaatta gttgatgacg gggctgtttc aaaagcgctt tttgatgatg
     4621 ccgttgcgat gaaagactcg agtagtgccg aagtttctgt ggctaaagcg aatcttgaga
     4681 aagctcaaat atcactgaac gatacggaaa ttcatgcccc gactcacggg acgatactaa
     4741 ccagaattca ggagccaggc gctattgtta atgtcgcgga accagtttat accctggcta
     4801 ttgataatcc ggtatgggta aggacttata ttgatgaacc ttttttaggt agaatttatc
     4861 ctggacagga tgctttggtt tacacggatt cgaatccgga aaagccctac aaaggtcata
     4921 ttggctttat ttccccgcag gcagaattca caccaaaaat tgttgaaacc actcaattga
     4981 gaacggatct tgtctatcgt ataagagtcg ttattgatca accggataat ggattaaggc
     5041 aaggaatgcc tgttactgtg aaaatccatt taaaagcgaa ttcatagtaa tgaatagttc
     5101 aaaaccacta gtcagtatta atggagtcag caaatgtttt cctggaaaca ctgatccggc
     5161 attagataat gtttctgcaa caatttttga agggcaaatc acgggtttgg ttggaccgga
     5221 tggggctggg aaatcaactc ttatgcgtct tatatgcagc ctgatgttgc ctacgcaagg
     5281 aacaattaca gtagacagtc tcaatacccg gactgactcg gaaaaaattc atgcgattac
     5341 tggctatatg ccacaaaaat ttggtttgta tgaagatttg accattatag aaaacttgag
     5401 gctctatgct gaacttagaa atctgcatgg cgagaaacgc gaagagcaat ttagaacatt
     5461 acttaaattt acagcactgg agccttttca gagacgtctg gctggtaatc tctcaggggg
     5521 aatgaagcaa aaattggggc tggcttgtgc cttaatgggt gagcctaagc tgttgttact
     5581 tgatgagccg agtgttggcg ttgacccaat atccagaagg gagttgtgga ctatggtcca
     5641 ggggctgctt ggaaggggta tgggcgttgt ttggtcaact gcttatcttg atgaagctga
     5701 aaagtgtgac caaattcttt tactcaatgc cggcaaacca atataccatg gaaaaccggg
     5761 agattttttg caaaaaacca aagaccggac ttttcaaatt tgcggtgttt ccctgctaaa
     5821 tcgtcgccaa gctctgatga atgcccttaa tacccctgaa gtcattgatg gtgtcatcca
     5881 gggtaagaat attcgtattg tcatcagtga taaaaaaata aagccaagtt tggatcttat
     5941 aactcgggaa cccggagttc attttcaaga agttgaatcg cgttttgaag acgcttttat
     6001 tgacagtctg aaaactaaaa taagtggtcg ctctttgtta gccgagcata ttgctgaaaa
     6061 gcctcatagt gacaagccta tcatcgaggc ccgaaagtta acgaaacgtt ttggtagttt
     6121 tactgccgtt tcgaataatg aatttacaat caaacgcggt gaaatctttg gtttattagg
     6181 ccctaatgga gcaggtaaat caaccacatt taaaatgatg tgtggtttat tgcagcccac
     6241 acaaggacaa gcatttgtaa tgggactcga tctaaagact tcatcctctg aagcaagaag
     6301 ccgtattggg tatatggctc agaaattttc actatacagt catttggatg ccttgcagaa
     6361 catgcgcttt ttttctggtc tctatgggtt atccggaaaa aggcaggaag cacagatcaa
     6421 cagcatgatt gaaatttttg atcttaaaaa gcacttaaag caaaattcgg gtgatcttcc
     6481 attaggattt aaacaacgtc tcgctctggc ttgtgccgta atgcatgagc ctgatgtgtt
     6541 atttcttgat gagccgactt cgggggttga tccattgacc cgacgtgagt tttggacaca
     6601 tattaatggt atggtgaata aaggtgtgac cgtgatggta accactcatt ttatggatga
     6661 agcggaatat tgcgatcgta tcgctcttat ttacaggggg aaaaacattg ccacaggaac
     6721 gcctgatgac ttaaaggata aagtacgtaa ctccacatta cccaatccca ctttagaaga
     6781 cgcgtttatc gagttaatcc gattggatga gttgcgtgag ccggtacatt aacacaggga
     6841 gaatagtggt tgaatattcg ctatctaaag gctttggtca ttaaggaaac tctgcaaatt
     6901 atccgtgatc ccagtactat attaattgca tttgtattgc ctttaatttt gctttttatt
     6961 ttcggttatg gtgttaatct ggacactgat caagtcaagt ttggcctggc tttagagagt
     7021 tatgattcgt cgactgtcag tctttctcaa tcttttagca gttcccgatt tttggatgta
     7081 tctatttcaa caaacagaca agcgtttcgt tatggtttgg ttaatggaac aattcgtggt
     7141 tttattgata ttcctcaacg tttcactgct gataacctga gtggaaatcc caagccagct
     7201 ctgcaaacga tagttgatgg ttcttatccg caagttgccg gttttgcaga aaattatgcg
     7261 cttggggctt tgcaaacctg ggttagctcc caagcttatg agcatgggca aatgacactt
     7321 aagtctggca ttaaccttga gcccagatac tggtacaacc aggagctaaa aagcagaaat
     7381 tttcttattc ctggctcaat agttattgtt atgactttga ttggaacatt actcacctcg
     7441 ctggtgatcg ctcgtgagtg ggaaagaggt acgatggagg cgatgatggc aacgccaatt
     7501 tctattgttg acatcatctt gggaaaatta ataccctatt ttgtgctagg gatgggttcc
     7561 atgtttttat gcactattat tgcaacatgg tattatggag tacctttcca aggtactctt
     7621 ttggcactga cattagtttc agctgtcttt ctggttgctg ctttagggca gggattatta
     7681 atatctactc ttgcaagaga ccagtttgtg gcttcgcaat tggctttaat gtctgcgttt
     7741 ttgccttcat ttattctttc cgggtttatt tttgaaatca attcgatgcc aaagtggata
     7801 caactactca ctcatttgtt tgctgcacgt tattttgtga caagtctgca aaccttgttt
     7861 ttaacgggta acatttggtc tcttttgatt cgttgccttt ttgcgattgc actgattgga
     7921 tttgcattct tttttattac cgcgcataaa accagaaaaa ggcttgatta atggcaaaat
     7981 tatctccaac aacaagaatt tttgctcttg tca
//
LOCUS       DAXLDT010000134         7954 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXLDT010000134 DAXLDT010000000
VERSION     DAXLDT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.134"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..240
                     /locus_tag="ACF2SP_002404"
     CDS             <1..240
                     /locus_tag="ACF2SP_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HFD2360178.1"
                     /translation="KKSISMHPDDVRWIKEVFSESNIPGLHELLVADPALNRGDFYLQ
                     GEHSELDGRIQTRLTTLFNEYINKENIIPMGVSQE"
     gene            243..1592
                     /gene="fliI"
                     /locus_tag="ACF2SP_002405"
     CDS             243..1592
                     /gene="fliI"
                     /locus_tag="ACF2SP_002405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFD2360179.1"
                     /translation="MAIYQTQLHKILSGTRNKHHSGLLHCGRISRAIGLTIEAKGFNQ
                     PVGARCLVKINDEHSVQAEVVGFSNESVYLMSIGDIQGIAPGMKIIPTGRIAQVSVGH
                     QLLGRILNGSGMFIDDGPPSEMVETYPLIGQVINPLKRAPIEVPLDVGIRAINGLLTV
                     GRGQRIGLFAGSGVGKSVLLGMMTRFTEADVVVVGLIGERGREVKEFIECNLGLQGLK
                     RAVVVAAPADESPLMRLHGAKVATSIAEYFRDQGKHVLLLIDSLTRFAQAQREIALSI
                     GEAPATKGYPPSVFAKLPKLVERAGNGTSGGGSITAFYTVLTEGDDLQDPIADAARAI
                     LDGHVVLSRALAEQGQYPAIDLEASISRVMQGVVSEQHMKDMLLFKKYLSVYEKNKDL
                     ILLGAYAKGSDPLVDKAILAVNKLKDYLAQGMDEQVGYDESLLSLSKLISTLVGNDQ"
     gene            1589..2044
                     /gene="fliJ"
                     /locus_tag="ACF2SP_002406"
     CDS             1589..2044
                     /gene="fliJ"
                     /locus_tag="ACF2SP_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFD2360180.1"
                     /translation="MSDRLDRLIQLLKIKQEATQQAYIELVKAREQFNQNKARHEQLV
                     GYRQDYLQQLEVLGQQGSYVGPLRNRINFINHLDTALVQLNSYLSQLAKNRMKADLNY
                     KQAKTSEEGINKLIERVKRTELKQLQRIEQKETDEYAQKQWYSSLKHDR"
     gene            2034..5855
                     /locus_tag="ACF2SP_002407"
     CDS             2034..5855
                     /locus_tag="ACF2SP_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HFD2360181.1"
                     /translation="MTDNNLLANKFFKKIWMGLAVLIIVAAVFSSVFRALTPWAKQYK
                     GEVEEHLTSLLGQPVTVQTMETGWYWFEPVVKLKHIRIHDGHNKSIYLDRLIVGVNLF
                     KSLWNWRIQPGVLYIDDMHLVFREKDGHWRIEGISRNAMKDTDITPEKTREILDWLAQ
                     QEKLIVRHVSAHLHFNDGSLIPVNELNLSIVNKGGNYKIKGEARLEQTNATVFQLLGN
                     VQFDPSQIENTNGRFYFSAINVLPAQWQNLLPKSSMQLEGGKGNVSLWVDLEKGNISS
                     IQAELRLKRLAWKLLNKKNSKLIQSFYANMGWKLDKQGWQFQADHMKLRVAGVTWPEN
                     QLSIRFNKNQNTYQIFTKSLIVESLLSEAINWPPAIQNVLQLKPHGILSDVQVLIKEN
                     QINYILTRFDQLGWRAKDNIPGVSNLSGVLNWQSMEGRLELDSENSTFDIKGYPTQKL
                     AIFNGAFDWKELNDGLRLSIERLVASQPELTISLEGAFDQLTQNSVGNIRLAGNFSGK
                     NLQKWMPYLPKKHMKAKLFAWLNNDIKRLGQATGKITINGMAKDFPFDNGNGEFSIVS
                     HVVGGDILITPEWRLIKDLEGYIKLKNRNLDIDIIDGDAQGTPLKQMNLRIDDIGKDR
                     ETLLIHGIVHGKAQQMLNFVMNSPLKKKLSALKILLIKGMLSLNLRLEIPLYPENDDN
                     LARGELTFENNIINIKHQMGDFSVRDVTGNLLFNEKGIQDSALTARAFGYPLNIKIQS
                     EKSPQPLTAISIEGECTVESLKKKLHLPIFSVLKGNILAKALLKITDDPNDLDNMKLS
                     SDLIGLAVDLPVPLGKTYKEKNPLQVNLDFNPQKTLRLRVNYNGRLSTDLLMQKQKGM
                     FKLKSGQIRLGSAQAVNQDHPGLGIAGTLDGFDLQAWKEVINRFSKENVNPSLLSLVN
                     IIDVKMSKLNFLNQQFDNMSLYAKKRPDNDWSVNLKQKKVDADLTYHSATNSLSGFVK
                     YLHLSEIGSSKNNKDTPSHATPGQIPNLNLRVDNFSIGKIQIGNITLKSHSTSEQWKI
                     DYCRIDSPYYQANFEGEWLQKGKFNQTKLQSKMYLKDLAKSLDRWAINPAVDAGKGYI
                     EFHGGWQGSLYDFSLSKLNGSMYLQLKNGRITHLSPETEEKLGLGKLLSILSLQTIPR
                     RLKLDFSDLSNDGYSFDIFKGNFNVNNGIMSTNNSYIDGPVAYASMKGDLDLVRRLYD
                     LDLNISPHITASLPVVATIAGGPIAGLAAWIANKIINQSMQKIAGYSYKISGPWDQPI
                     VQQLNIVKKLP"
     gene            5908..6528
                     /locus_tag="ACF2SP_002408"
     CDS             5908..6528
                     /locus_tag="ACF2SP_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360182.1"
                     /translation="MEEYKIFCFVQRALKPQENNQKIIMNTLKLLFSYYWRLSLLKET
                     PENSPYSVFLLIVMSVLFSIIMLIQWDIAELDFSRDWSLSLMMATSLILSFIFYTYVI
                     LKFQNLASRLVQTSTCLLSAYIIIHVLATPLFLIDTYLSAENLKTPIFLFIGMIYLFI
                     TLGLSIWQFVITAHIYKFALGTTPVKSVLAAFGLVAVNILTISFWR"
     gene            6530..7897
                     /gene="mpl"
                     /locus_tag="ACF2SP_002409"
     CDS             6530..7897
                     /gene="mpl"
                     /locus_tag="ACF2SP_002409"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFD2360183.1"
                     /translation="MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLL
                     AAKGISWIEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENIL
                     SKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
                     ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGV
                     AIKPRDDKALNEVLSRGQFSRIETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVS
                     WPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSPVKRRLEVRSNRHGVTVYDDF
                     AHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAGEMAHALAPVDGAYVLEP
                     QDFNLKDFVSSWTCPYKICKNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSI
                     DKLFM"
BASE COUNT         2534 a         1270 c         1718 g         2432 t
ORIGIN      
        1 aagaagtcta taagcatgca tcctgatgat gtgcgatgga ttaaagaagt attttctgaa
       61 agtaatatcc ccggtcttca tgagttattg gtggctgatc cagcattaaa tcgaggagat
      121 ttttatttac aaggtgaaca cagcgagtta gatggtcgaa ttcaaacgcg tttaactacc
      181 ttattcaatg aatatattaa taaagagaat ataatcccaa tgggcgtttc tcaggaatag
      241 tgatggccat ttaccaaaca caactccata aaattttgtc aggaacaagg aataaacatc
      301 attctggact gttacattgt ggtagaatca gcagagcaat aggtttaacc atagaagcaa
      361 agggatttaa tcagcctgta ggagcgcgtt gcctggttaa aatcaatgat gaacattctg
      421 ttcaggcaga agttgttggt tttagtaatg aaagtgttta tttaatgtct attggtgata
      481 tacaaggtat agcccctgga atgaaaatta ttcctaccgg acgcattgct caagtaagtg
      541 ttggccatca attgttagga agaatattaa atggttcggg aatgtttata gacgatggac
      601 ctcctagtga aatggttgag acttatccat tgatagggca agtgatcaat cctttaaaaa
      661 gagcccctat tgaagttcca ttggatgttg ggattcgtgc tattaatggc ttattaactg
      721 ttggccgcgg acaacggata ggattgttcg ctggaagtgg agttggcaaa tcagttttgc
      781 ttgggatgat gacgcgtttt actgaagctg atgttgttgt ggttggatta attggagaac
      841 gtgggcgcga ggtaaaagaa tttattgagt gcaatttggg tctgcaaggt ttgaagcgag
      901 cggttgtagt tgccgctcca gcggatgaaa gccctttgat gcgtttgcac ggcgcgaagg
      961 ttgcaacaag cattgctgag tattttcggg atcaaggtaa acatgttctc ttgctgattg
     1021 attctttaac ccgttttgcc caggcacaaa gagaaatcgc gttatcgatt ggtgaggctc
     1081 ctgctacaaa aggatatccg ccatcggtat ttgccaaatt gcctaaattg gttgaaagag
     1141 ctggaaatgg gacttcgggg ggtggttcaa ttacggcatt ttatactgtc ttgactgagg
     1201 gagatgattt gcaagatccc atagcagatg cggcacgagc tattttggat ggtcatgttg
     1261 tattaagcag agcgcttgca gagcaagggc agtatcctgc aatagatctt gaagcttcta
     1321 ttagccgagt tatgcagggc gttgtctctg aacaacatat gaaagatatg ttattgttta
     1381 aaaaatactt gtctgtttat gaaaaaaaca aggatttaat tttattagga gcctatgcaa
     1441 aaggaagcga tcccctggtg gataaagcga tattagcagt gaataaactc aaagattacc
     1501 tagcgcaagg aatggatgaa caagtaggct acgatgagag tttattatcg ttatccaaac
     1561 tgatttcaac tttggtaggg aatgaccaat gagtgacaga ttagatcgtt tgattcagtt
     1621 attaaaaatt aaacaggaag cgactcaaca ggcctatata gagttagtga aagcaagaga
     1681 gcaatttaat caaaataaag caaggcatga acaattagtt ggttacagac aagattattt
     1741 acaacaactg gaagtattgg ggcaacaagg aagttacgta gggcctttac gcaaccgaat
     1801 taattttatt aatcatttgg atactgcttt ggtacaatta aactcctatt tgagtcaact
     1861 tgcaaaaaac aggatgaagg cggatttaaa ttataagcaa gcaaaaactt cagaagaagg
     1921 aataaataag ttaattgaac gcgtaaaaag aactgaactg aagcaattac agcgtattga
     1981 gcaaaaggaa actgatgaat atgcgcaaaa gcaatggtat agtagtttaa aacatgaccg
     2041 ataataacct tttggcaaat aaatttttta aaaaaatttg gatgggattg gccgtcctta
     2101 taattgttgc agctgtcttt tccagtgtat ttcgcgctct tactccctgg gctaaacaat
     2161 ataaagggga ggtagaagag catttgactt cacttttggg gcaaccggtt actgttcaaa
     2221 ctatggaaac agggtggtac tggtttgaac cagtagtcaa attaaaacat ataagaattc
     2281 atgatggtca taataagtca atttatctcg ataggttaat tgtcggtgtt aatttattta
     2341 aatccctatg gaattggcgc attcaacctg gcgtacttta tatcgatgat atgcatttag
     2401 tttttcgtga aaaagacggg cattggcgta ttgagggaat ttcacgtaat gcaatgaaag
     2461 acactgatat tacacctgaa aaaaccaggg aaatattaga ttggcttgct cagcaggaaa
     2521 aattaattgt acggcatgtt tccgctcact tacattttaa tgacgggtca ttaatccctg
     2581 ttaatgaatt aaacctgtcg atagtgaata aggggggaaa ttataagata aagggggaag
     2641 caagactcga acaaacgaat gcaaccgttt tccaactgct agggaatgta caattcgatc
     2701 cttctcaaat tgagaacacc aatggacgct tctatttttc agctattaat gttttacccg
     2761 cacaatggca aaatttattg ccaaaatcct caatgcaact ggagggaggt aaaggaaacg
     2821 tttcattatg ggttgatttg gagaaaggga atatttcatc aattcaggca gaattgagac
     2881 ttaagcgtct ggcatggaaa ttattaaata aaaaaaatag taagttgatc caatcatttt
     2941 atgccaacat gggctggaaa ctggataaac aagggtggca gttccaggca gaccatatga
     3001 aactaagagt agcaggggtc acttggccag agaaccaatt gtcaattcgt tttaataaaa
     3061 atcagaatac ctatcaaatc tttacaaaat cccttattgt agagtcacta ttatccgaag
     3121 cgattaattg gccgccggct attcaaaatg tactgcagtt aaaaccgcat ggaatattga
     3181 gtgatgttca ggtattgatc aaagaaaatc aaattaatta tattttaact cgatttgatc
     3241 aattaggatg gagagcaaag gataatattc ctggagtgag taatttatcc ggcgttttaa
     3301 attggcagtc tatggaagga cgccttgagt tagattccga gaattcaact ttcgatatca
     3361 aaggttaccc tacccaaaaa ctggcgattt tcaatggtgc ttttgattgg aaagagctaa
     3421 atgatgggtt gcggttgagt attgaacgct tggtcgcaag tcaacccgaa ttgaccatta
     3481 gtctggaagg cgcttttgat cagctgaccc aaaattctgt aggcaatatt cgtttagcgg
     3541 gtaatttttc tgggaaaaat ttacagaagt ggatgcctta tttgcctaaa aaacacatga
     3601 aagccaagtt atttgcttgg ttaaataatg acatcaaacg tttagggcaa gcaacaggca
     3661 agataactat taatggaatg gcgaaggatt ttccatttga taatggcaat ggggagtttt
     3721 ctatagtaag ccatgttgtc ggtggagata ttttaattac tccagaatgg cgattgatta
     3781 aagacttgga aggttatatt aaactaaaaa atagaaatct tgacattgat attattgatg
     3841 gtgatgctca aggaactccg cttaaacaaa tgaatttgcg tattgatgat attggcaaag
     3901 atagggaaac cttattaatt catggaattg tgcatgggaa agcacaacaa atgctcaatt
     3961 ttgttatgaa ttccccatta aagaaaaaat tatctgcttt aaaaatatta ttaataaagg
     4021 ggatgctatc actcaatttg cgtttggaaa tacctcttta tccagagaat gatgataatc
     4081 tggcccgagg tgaattaacc tttgaaaata atataatcaa catcaagcat cagatgggag
     4141 atttttctgt aagggatgtt actggaaatt tactgtttaa tgaaaaaggc atccaggata
     4201 gtgccttaac tgctagggca ttcggctacc ccctgaatat taaaattcaa tcggaaaaat
     4261 ctccacaacc attaaccgca atatcaattg aaggtgaatg cactgtagaa tcgctaaaga
     4321 aaaaattgca cttacctatt ttttctgtac tcaaagggaa tattttagct aaggcattat
     4381 taaaaattac cgacgatcct aatgatttgg ataatatgaa gttgagttcc gatttgattg
     4441 gattggcagt tgatcttcca gtacctttag gaaaaacata taaagaaaaa aaccccttac
     4501 aggttaatct ggactttaat ccacaaaaga ctcttcgttt aagagttaat tacaatggac
     4561 gactaagtac ggatctattg atgcaaaagc aaaaaggcat gtttaagtta aaatcaggtc
     4621 aaatacgttt aggtagtgcg caagctgtaa atcaggacca tcctggcctc ggtatagctg
     4681 gtactttaga cggctttgat ttgcaggcat ggaaagaggt aataaacaga ttctctaaag
     4741 agaatgtgaa cccctcactc ttgagtttgg ttaatattat tgatgtaaaa atgagtaaac
     4801 taaacttttt gaatcagcag tttgataaca tgtctttgta tgcgaaaaaa cgccctgata
     4861 atgattggtc agtcaattta aaacagaaaa aagtcgatgc ggatttaacc taccattcgg
     4921 caaccaattc tctgagtggc tttgtcaaat acttacattt atctgaaatc ggatcttcaa
     4981 aaaataataa agatactcct tcacatgcta ctcctggaca aatccccaat ttgaatttaa
     5041 gagttgataa tttttcaatt ggaaaaattc aaattgggaa tatcaccctt aaaagtcatt
     5101 ctacatcaga acagtggaag attgattatt gcaggataga ctcaccttat tatcaagcta
     5161 attttgaagg ggaatggctt cagaaaggga agtttaatca aacaaagcta cagtctaaaa
     5221 tgtatttgaa agatttggcc aagagtcttg atcgttgggc gattaatcca gcggtggatg
     5281 cagggaaggg atatatagaa tttcatgggg gttggcaagg ttccttatat gatttttctc
     5341 tgtccaagtt gaatggttcc atgtatttac agttgaaaaa tggccgcatt actcatttga
     5401 gcccggagac tgaagagaaa cttggtttag ggaaattatt aagcatattg agtttgcaaa
     5461 ctattccaag acggttaaaa cttgatttta gtgatctctc aaatgatggc tacagttttg
     5521 atatatttaa aggcaacttt aatgtcaata atggcattat gagcaccaat aacagttaca
     5581 ttgatggccc agtagcttat gctagtatga aaggggatct ggatttggta agacgattat
     5641 atgatttgga tttaaacatt tcaccgcata tcactgccag cttacccgtt gtagctacga
     5701 tagccggagg tccgattgct ggtcttgctg catggattgc aaataaaatt attaatcaaa
     5761 gcatgcaaaa aattgcggga tatagttata aaatttctgg cccttgggat cagcctattg
     5821 tgcaacagct taatatagtg aagaaattgc cttaactaaa ggaagacaag attatttagt
     5881 aaacagttat gaagtcactt agggcccatg gaggaatata aaatattctg ctttgttcag
     5941 agagcgctta agcctcaaga aaataatcaa aaaataatta tgaatacatt aaaattacta
     6001 ttttcttatt actggcgtct tagcctattg aaggaaaccc cggagaattc tccttattcc
     6061 gtttttcttc ttatagtaat gagtgttctg ttctctatta taatgctgat tcaatgggat
     6121 atcgccgaac ttgatttttc ccgcgattgg tcattaagct taatgatggc tacgagcctg
     6181 attttgtctt ttatattcta tacctatgtt attttaaagt ttcaaaatct tgcgtccagg
     6241 cttgtacaaa catcaacatg tttattgagt gcctatatta ttatacatgt tttggcgaca
     6301 ccattgttct taattgacac ttatttgtct gcggagaatc ttaaaacccc cattttttta
     6361 tttataggta tgatatattt gttcattact ttggggcttt ctatttggca atttgtaatt
     6421 acagcccata tttataaatt tgctttaggc actacaccgg taaaatcagt tttagctgct
     6481 tttggtttag tcgctgttaa tattttaact atttcttttt ggcgatgaca tgatgacaca
     6541 tttgcatata ctaggagtaa gtggaacctt tatgagtgcc ttggctttac tcgctcgcga
     6601 cgcgggatat agagtgacag gcagcgatgc caattgctat ccacctatca gtgatttgct
     6661 tgctgccaaa ggcatttcat ggattgaggg ctacgatgat tccactttag cattgcaatc
     6721 tgacatggtt atagtcggta atgctattaa gcggggaatg ccggttcttg aagcagttat
     6781 ggagtcggga aaaccatata tgtcaggccc tcaatggctt gccgaaaata ttttatcaaa
     6841 atatcaggtg attgcggtag ctggaactca tggcaaaacg actaccactt caatgattgc
     6901 ctggattttg gatcaagctg gaatgaatcc gggatttctc attgggggcg tctctagtga
     6961 ttttaatacc agtgcctgcc ttggcaaagg gagatggttt gtcatagagg cagatgagta
     7021 cgatagtgct ttttttgata aacgacctaa atttatgcat tatcgaccaa atattgcagt
     7081 attaaataat ttagaatttg atcatgccga tatttatccg gacttggcag ctattcaaca
     7141 acaatttcat tatttattaa gaacaattcc caaaagtggt gtggctatca aacctcgaga
     7201 tgataaggct ctgaatgaag ttctatcacg aggtcaattt tcgcgtattg aaacattagc
     7261 actatctgga gaggccgaat gggttgccga actgattgac gatagtggtt cacaatttaa
     7321 aatactccat aatgggggag tggttgctga agtatcttgg ccgttgatag ggcagttcaa
     7381 tgtagaaaat ggtttggctg caattgctgc tacctggaat gctggagtaa atcctgtggt
     7441 tgcagccaag gctttagaac agttttctcc agtgaaacga aggcttgaag ttcgttccaa
     7501 tcgacatgga gtaaccgttt atgatgattt tgcccaccat ccaaccgcaa ttactaaaac
     7561 tattgatgct ttaaaaagaa gtggacgaca tcaacgtatt tttgctgtta tggagtttgc
     7621 ttcatatact atgagaacgg gtgtacatgc cggagaaatg gctcatgctt tagctcctgt
     7681 tgatggtgcc tatgtcctgg agcctcagga ttttaattta aaagattttg ttagctcctg
     7741 gacttgcccg tataaaattt gcaaaaacaa tggagagata gttaaagaaa ttatcaaaac
     7801 agtaagagag ggcgatgctg tattggtaat gagtaataga ggatttaatg gaattcatca
     7861 acaattgatc gattctatag ataaactttt tatgtaacga ttcattttta ttctgaaatg
     7921 tcttgagaac atataatgat attgagaagc aggt
//
LOCUS       DAXLDT010000135         7740 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXLDT010000135 DAXLDT010000000
VERSION     DAXLDT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.135"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..381
                     /locus_tag="ACF2SP_002410"
     CDS             64..381
                     /locus_tag="ACF2SP_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HFD2360184.1"
                     /translation="MTRKLRIEEKLISALSPLFLSVEDESMNHHVPQGAETHFKIIAV
                     SNHFNDLTRINRHKFVNQLLKEEFTLGLHALSMHLYTSDEWITQQQSVLKSPACRDGY
                     KNK"
     gene            456..1877
                     /locus_tag="ACF2SP_002411"
     CDS             456..1877
                     /locus_tag="ACF2SP_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD2360185.1"
                     /translation="MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIF
                     SAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTI
                     LSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
                     VPMGFIILLAFIWLIHGHPIAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVH
                     VRNVNNPQKNFPKAMFFSVILILFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQV
                     YDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPASLCHVNKHGMPEKI
                     LILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDME
                     RPFAIPGGKPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGILLML
                     LPAALIYLRKRHITKSKALNAPV"
     gene            complement(1983..3413)
                     /locus_tag="ACF2SP_002412"
     CDS             complement(1983..3413)
                     /locus_tag="ACF2SP_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HFD2360186.1"
                     /translation="MKIFKFSSWLLLFVFTLDANAYYTSQGSIYDKSGNRVTINGVSW
                     SGFQDTNVFQGLQSNPFYNITMPQSKPRNGLMDLLVHPWSMADSGVNSSTAVEFKTVR
                     LPIQPGVLYDDSGEVDLNKWLSDKTQPEAGNGLFCKTWQSNGQSCEKAVSPKQAFWTV
                     LSEMKKHNINVMIDFHHRYGYGDGMRDGTVYSMAQYEKDLVLLAQEIKQRGLDNVIGI
                     DVFNEPYQLSWFKARNGQVSWITVIATAAKAVHQVNPDLLLFVEGPGGGNDDMDNPTI
                     CVPKANIKEDSGYAHWRDPGQCGNDQEVVAFKGNWGEDFKPLLDKNLAKQNIAQFDVQ
                     KFSNELQLAVPGIDTETLGWLLGDDNMGNNGHLVFSPHVYPKEVAGWETAPGDASNLR
                     FDWSWGFLYKAGYPIVLGEASWKSFEGKNFFTQALMPYLTQSGIGSNNLYFWAIGYLG
                     DTVSAINPNTGELNLDVQQTLKPYFN"
     gene            complement(3661..4413)
                     /gene="kdsB"
                     /locus_tag="ACF2SP_002413"
     CDS             complement(3661..4413)
                     /gene="kdsB"
                     /locus_tag="ACF2SP_002413"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFD2360187.1"
                     /translation="MSHNFHVIIPARYHSSRFPGKLLQEINGITVIERVYRQALLAEP
                     KSVIIATDHDEIADRAIQFGAEVVITSHTHQTGTDRIAEVVAKGSFAPDDVIVNVQGD
                     EPFIRPKLIQQVACSLTKTKAPVSTLCWPISSLQILNNPNVVKVVCTRDNHALYFSRS
                     AIPFHRDDKNAYSNTFRHIGLYAYRAAFLLEFVSWPPCALEQIECLEQLRILWSGFSI
                     RVDEACEEPLQDINTKEDLILAQQYFLDTFNV"
     gene            complement(4410..4589)
                     /locus_tag="ACF2SP_002414"
     CDS             complement(4410..4589)
                     /locus_tag="ACF2SP_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019231979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFD2360188.1"
                     /translation="MDKKLLEILVCPLCKGKLLFKKQELICKFDRLAFPVRDDIPVML
                     EQEARLIPLEEKDKL"
     gene            complement(4582..5253)
                     /gene="tsaB"
                     /locus_tag="ACF2SP_002415"
     CDS             complement(4582..5253)
                     /gene="tsaB"
                     /locus_tag="ACF2SP_002415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFD2360189.1"
                     /translation="MMKLLAIDTSTELASVAILIGDEIISREQDSQRIHAQLILPMVD
                     ELIAQAGLGLNQLDGIIFGCGPGSFTGLRIACSIAKGLAYANDLPLVPVSSLAAIAWT
                     AREIKEDFNQPVLSVLDARMHEMYWSCFLEQQFLAQDRINAVKDIQLPANQSFILAGV
                     GIDLYWKDFPEQIKSQISEVLTVFPTASAMIRLTHKANIKAVSVAQAQPVYVRNQVTQ
                     GDSRG"
     gene            complement(5231..7189)
                     /locus_tag="ACF2SP_002416"
     CDS             complement(5231..7189)
                     /locus_tag="ACF2SP_002416"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFD2360190.1"
                     /translation="MEEITTFAETCRRTLSEKGRLSTAIPGFVARAPQADLAVAIANA
                     IVQRSILVAEAGTGTGKTFAYLLPCLLSGKKALISTATKTLQDQLFQKDLPTLIRALG
                     LSVRIQNLKGRSNYICQYRVERHAEEGQFQSPQCAHEILHVRDKLSQMQEGVRSELPE
                     INEDSSVWHYVTSTTDNCLGAECPNHETCFLVKARKRAMEADIVVINHHLFFADSQLK
                     KEGFSELLPGVDVVVFDEAHQLADIASNFNGERLGTRQFKDLVDDMLNEWPILDLANQ
                     PLKSISHKADKLVDQLLNALPTREERISWELVKRNKEFMEAWNTWLSLKDELIQCLES
                     KDTVDIPGLKRCQERLLDLETVLLSFTEENNHKIRWLERFKHTLVFHATPYDVAESFS
                     QLLKDQSCAYIFTSATLTMASAFDSFCKPLGLDKVQTLLLSSPFDYQQQALLYLPRGI
                     PDPKDAHYYEALLEKALPVIEACGGRCFFLFTSHKAVKLVAQMMNNTLKYPLLIQGDE
                     AKPILLSRFRQLGNAVLLGTSTFWEGVDVKGEALSCVIIDKLPFANPVDPVIRGRMAY
                     LKKKGLSGFDELSLPNAVIALKQGVGRLIRDITDKGILMIGDPRLTGREYGRTILASL
                     PGLPKTRDEEKVLHFIKELALNDETVSD"
     gene            complement(7177..7512)
                     /gene="fdxA"
                     /locus_tag="ACF2SP_002417"
     CDS             complement(7177..7512)
                     /gene="fdxA"
                     /locus_tag="ACF2SP_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HFD2360191.1"
                     /translation="MTFVVTESCIRCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVNAIVSEDDLTEEQQQFKKLNAELSKTWPNITAKKDAPSDAKDWEEVKDKL
                     QYLQKEWKK"
     gene            complement(7546..>7740)
                     /locus_tag="ACF2SP_002418"
     CDS             complement(7546..>7740)
                     /locus_tag="ACF2SP_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360192.1"
                     /translation="NRFKMIKITIFAARLQVHFMSSKNPYDAVGFEFMISGWFQIMNG
                     TRGIKISGSSIINRVFYALI"
BASE COUNT         2313 a         1691 c         1391 g         2345 t
ORIGIN      
        1 atgtgacaac tatgcttcaa atgtatatac tccttatttt gcaaaaacag agccgagcaa
       61 atcatgacgc gtaaactccg catagaagaa aaactaatct ccgcattatc ccccctcttt
      121 ttaagtgttg aagacgagtc catgaaccac cacgttcctc aaggcgctga aacacatttt
      181 aaaataatcg ccgtttcaaa tcattttaat gatctgactc gtattaatcg tcataaattc
      241 gttaatcagc ttcttaaaga agaatttact ctgggactgc atgcattaag catgcactta
      301 tatacttctg atgaatggat aactcaacag caatcggtac tcaaatcccc tgcttgcaga
      361 gatggctata aaaacaaata attttaatga aaaaagttca cctccaagct tgatttattt
      421 tataatgtct atctttttat caaatagaaa aatcaatgaa atcatggtcc tctgaaaaaa
      481 tatccgttct tgctttagtg ctgctcatta ccggagcaat tgacagcata agaaatttgc
      541 caggcacagc actttttgga tctactttga tttttttctt tattttttca gcgattatct
      601 ttttgatccc ggttgcttta gtcgctgctg aactttcctc aacctggtca gacgaggaag
      661 gtggcattta cagttgggtc aagcatgcct ttggtgaaaa catcgccttt ttcgccattt
      721 ggctgcaatg gataaatact ctggtatggt atccgactat tttgtctttt atagcaggca
      781 caatctctta tttaattaat ccagaacttg cacaaaacaa atactatctt atcagcgtta
      841 ttctgattat tttctggtca ttaactttga taggcttatc agggctgcgt gcttctgcca
      901 cctttgctgg cttctgtgca atagttggaa tgatagtgcc tatgggattt atcatccttc
      961 tggctttcat ttggttaatt catggccacc ctattgccat tgatctgagc ctctccaatt
     1021 tgctaccaca gtggaaaaac acacaatcct gggtttctct aacagcgatt atgacttctt
     1081 ttctcgggat ggagcttgct gcagttcacg tgcgtaatgt caataacccg cagaagaatt
     1141 ttcccaaagc gatgtttttt tcggtcattc tcatcctgtt cactatgatt ttaggctcac
     1201 tggccattgc tttcattctg cctaaagaca aaatcagttt ggtagatggg gttatgagag
     1261 catttagtaa tttcttgcaa gtttatgatt tgaattggct aatgcctgta ttaatactct
     1321 tgttattgct gggtacctta ggcagcatga tcaactggat tatttctcca gccaagggtc
     1381 tgctgatggc agccaaacat gattttctac ctgcttcgct atgtcatgtg aataaacatg
     1441 gcatgccaga aaaaattttg atactacaag ccattctggt gacagtatta tgcagcggct
     1501 ttctcttatt ccctacagtg aatgctattt attggttgtt tactgctctt agtacagagc
     1561 tgtatatgat gatgtatgtg atgatgttta ttgcagcctg gcatttgaag agtaaattct
     1621 cagacatgga aagacctttt gctattccag gaggcaaacc cggttattac ctgacatgct
     1681 tgttaggatt aataggttgt actgtcactt tatgggtagg ttttattcct ccaacggaaa
     1741 gcattaatat cggaggaaca gcccattacc gtatggtctt ttcattaggc atcctcttaa
     1801 tgctgctacc tgctgccttg atctatttgc gtaaacgcca cattaccaaa tccaaagcgc
     1861 ttaatgcacc tgtttaagta gccgatagac cgataggatg attgtaacct taacaataat
     1921 taggaaccgt atatattatc aggagtggcc ataaataaga gccactcctg aaacgagatg
     1981 tattaattaa aataaggctt caatgtttgt tgtacatcga gatttaattc gccagtattc
     2041 ggattgatag cactaaccgt atcacctaaa taccctatcg cccagaaata taaattattg
     2101 gacccgatgc cgctttgggt aagataaggc atcaatgcct gggtaaaaaa gttttttcct
     2161 tcaaaagatt tccaggaggc ttcccctaaa acaataggat acccagcttt gtacagaaaa
     2221 ccccaactcc aatcgaaacg cagattactt gcatcaccag gggctgtttc ccaaccagcc
     2281 acttcttttg ggtaaacatg aggtgagaaa accagatggc cattatttcc catattatca
     2341 tcacccaaca gccaaccgag tgtttctgtg tcaatacccg gaacagccag ttgcaattca
     2401 ttactaaatt tttgtacatc aaattgcgct atgttttgtt tagccaaatt cttatccaga
     2461 aggggtttaa aatcttcccc ccagttccct ttgaatgcta caacctcctg atcattgcca
     2521 cactgccctg gatcgcgcca gtgtgcatag cctgaatcct ctttaatatt ggcttttggc
     2581 acacaaattg ttggattatc catatcatca tttccgcctc caggtccttc aacaaacagt
     2641 aacaaatcag gattgacctg atgcactgcc ttggcagcag tagctatcac tgtaatccac
     2701 gaaacctgac catttctggc tttaaaccag ctcaattgat agggttcatt aaaaacatca
     2761 atgccaatga cattgtccaa accacgttgt ttgatttcct gagcaagcag aactaaatcc
     2821 ttctcatatt gagccatgct ataaacagtg ccatccctca ttccatcgcc ataaccataa
     2881 cgatggtgaa aatcaatcat cacattgata ttatgttttt tcatttcaga aagtaccgtc
     2941 cagaaagcct gcttcgggct cactgctttt tcacacgatt ggccattgga ctgccaggtc
     3001 ttgcaaaaaa gaccattacc ggcttcaggt tgtgttttat cagaaagcca tttgttgaga
     3061 tcgacttccc cactgtcatc ataaagaaca cctggctgta taggcaaacg tacggtcttg
     3121 aactctactg ccgtgctgct gtttacacca gagtctgcca tagaccaggg gtgaaccaat
     3181 aaatccatta atccattccg aggtttggat tgaggcatgg tgatattata gaaaggattg
     3241 ctctgcaatc cctgaaaaac attggtgtcc tgaaatcctg accaggatac tccattgatg
     3301 gtcaccctat tcccactttt gtcatagata ctgccttgac tggtataata ggcattcgca
     3361 tccagggtga atacgaacag caaaagccaa ctgctaaact taaatatttt catgcgcttt
     3421 tcctcattaa aataaccata aagttattgt taagttggtt aaaagctcat tatttaacca
     3481 caaaactaca atatttgaac agtataatca ttaatttttc aaaaatatta caaaaagatg
     3541 tcatttcgat gtgctgaaaa atagtcaaga tcgtcactcc attatgatga aaattgagtg
     3601 gcgaccatat ggagtgaata aaatcattcc ttcatagaaa aagcaataaa ttacaatgag
     3661 ttatacattg aaagtatcaa gaaaatattg ctgagccaaa atcaaatctt cttttgtatt
     3721 aatatcctgt aaaggttctt cgcaggcctc atcaacccgg atagaaaaac ctgaccatag
     3781 gatgcgcaat tgctccaaac actctatttg ttccaaggca cagggtggcc agctaacgaa
     3841 ttccagtaaa aaggcagcac gataagcata taaaccgata tgcctgaaag tattggaata
     3901 agcgttttta tcatctctgt gaaaaggtat cgcgcttctt gaaaaataaa gagcgtgatt
     3961 atctcgagta cataccactt taaccacatt agggttattt aatatttgaa ggctgctaat
     4021 aggccagcat aaagtagaca caggcgcctt tgtctttgtt aaagagcaag caacttgttg
     4081 aattaatttg ggtcggataa atggctcatc accttgcaca ttgacaatga cgtcatcagg
     4141 agcaaagcta cccttggcga caacctctgc aattcgatca gttccagttt ggtgcgtatg
     4201 tgaagtgatt acaacctccg ctccaaattg gattgcacgg tcagctatct catcatgatc
     4261 agtggcaata atcaccgact ttggttctgc cagaagtgct tgcctgtaaa ctcgttcaat
     4321 cacggtaata ccgttaattt cctgaagtaa tttacctgga aaacgactgg agtgatatct
     4381 ggctggtata atcacatgaa aattatggct cataatttat ctttctcttc cagaggaatc
     4441 aaacgcgctt cttgttccag cattacagga atatcatcac gaacaggaaa tgccagccta
     4501 tcaaatttgc agatcaattc ctgttttttg aacagcaatt ttcctttaca taaaggacaa
     4561 accaatattt ccaataattt tttatccacg cgaatctcct tgagtaacct gattgcgcac
     4621 atacactggt tgtgcctgcg ccacagaaac tgccttgata tttgctttat gtgtcaaacg
     4681 tatcatagca gacgctgtcg gaaaaacagt taatacttca ctaatttgtg atttgatctg
     4741 ttctgggaaa tccttccagt acaaatcaat cccaactccg gccaaaataa aggattgatt
     4801 ggctggcagc tgaatatctt ttactgcatt aattctgtcc tgagctaaga attgctgctc
     4861 caaaaagcaa ctccaataca tttcatgcat acgagcatcc aaaacagaca acacaggctg
     4921 attaaaatcc tcttttattt cccgagctgt ccaggcaatt gcggctaaac tactcacagg
     4981 aacaaggggc aaatcattgg cataagccaa cccttttgca atgctacacg cgattctcaa
     5041 tcctgtaaaa cttccaggac cacatccaaa aattattcca tccaattgat taagacccaa
     5101 tccggcttgg gctatcaatt catcaaccat aggtaaaatc aattgggcat ggattctttg
     5161 actatcctgc tccctgctta tgatttcgtc acctataagg atggcaacac tggccagttc
     5221 agttgaggta tcaatcgcta acagtttcat catttaaagc caactcttta ataaaatgca
     5281 gtactttttc ttcatcccga gttttcggaa gcccaggtaa acttgccaaa atggttcgac
     5341 catactctct accagtcaaa cgtggatctc ctatcattaa tataccttta tcggtgatgt
     5401 cacgaatcaa tcgccctact ccctgtttta atgcaatcac ggcgtttggc aaggataatt
     5461 catcaaaacc ggaaagccct tttttcttta aataagccat tctgccacga attacaggat
     5521 caacggggtt agcaaagggt aatttatcta taatcacgca agaaagtgcc tctcctttta
     5581 catcaacacc ttcccaaaaa gtagaagtac ctaaaagcac agcattgccc aattgccgga
     5641 atcgagacag gagaatgggt tttgcttcat caccttgaat caaaagcggg tattttaaag
     5701 tattgttcat catttgagca actaatttta ctgctttatg actggtaaaa aggaaaaagc
     5761 aacggccgcc gcaagcttct ataactggca aagccttttc cagcaaagct tcataataat
     5821 gagcatcttt aggatcgggt attcctcgag gcaaataaag cagagcctgt tgctgataat
     5881 caaatgggct ggataataac aaagtttgta ctttatccaa tcccagaggt ttacaaaaac
     5941 tatcaaaagc cgatgccata gtcagtgtag ctgatgtaaa aatataggca catgattggt
     6001 cttttaataa ttgactaaat gattcagcaa catcatacgg ggtggcatga aaaacaagag
     6061 tatgtttaaa acgttctaac caacgaattt tatgattatt ttcctcagtg aaagaaagca
     6121 agacagtttc caaatccaaa aggcgctctt gacatctttt taacccggga atatcaacag
     6181 tatcctttga ttccaggcat tgtatcaact catcctttaa agataaccag gtattccacg
     6241 cctccataaa ctccttattt cgtttaacca actcccaact gattcgctcc tcacgagttg
     6301 gcaaagcatt gagcaattga tccaccagct tgtctgcttt atgacttata gactttagag
     6361 gttgattggc caaatccagg attggccact cattaagcat atcatcgacc aagtctttga
     6421 attgacgtgt tcccagacgc tccccattga aattagaggc gatatctgca agctgatgag
     6481 cttcatcaaa cactacgaca tcaacaccag gtaatagttc gctaaaacct tcttttttta
     6541 actgagaatc agcaaaaaaa agatggtgat tgataacgac aatatctgcc tccatagctc
     6601 gcttacgcgc tttcactaga aaacaggttt catgatttgg acattctgca ccaagacagt
     6661 tatcggtagt ggatgtgaca taatgccata ctgaggagtc ttcattaatt tcaggtaatt
     6721 ccgagcgaac gccttcttgc atttgagaca atttatctct gacatggagt atttcgtgag
     6781 cacattgcgg gctttgaaat tgtccttcct cagcgtgcct ctcaactcga tattgacaaa
     6841 tataattgga acggcccttt aaattctgga tccgtaccga taaacccaaa gctctaatca
     6901 aggttggcaa atctttttga aaaagttgat cttgaagcgt ttttgttgct gtagagataa
     6961 gcgctttttt accacttaac aaacaaggca ataaataagc aaaggtctta cctgttcctg
     7021 ttcctgcctc agctaccaaa atcgatcgct gaacaatagc attggctata gctactgcca
     7081 aatcggcttg tggtgctctt gccacaaagc ctggaatagc agttgatagt cttcccttct
     7141 cactgagagt tcgccgacag gtttcagcga aagtggttat ttcttccact ctttttgcaa
     7201 atattgaagt ttgtccttta cttcttccca atccttggca tcagagggag cgtccttttt
     7261 agcagtaata ttaggccagg ttttggataa ctcggcattt aattttttga attgctgttg
     7321 ttcctctgtt aaatcgtctt ctgaaacgat agcattaact ggacattcag gttcacaaag
     7381 ggcacaatca atgcattcat cggggtgaat caccaggaaa ttaggcccct cataaaaaca
     7441 atctacaggg cacacttcta cacaatcggt atacttgcat cgtatacaac tttccgttac
     7501 tacaaaagtc atagcaaacc ttaaaaatta tctttaagaa atgactcata ttaaagcata
     7561 aaataccctg ttgatgatag aggagcctga tatttttata cctctggttc cattcattat
     7621 ttggaaccaa cctgaaatca taaactcaaa accaacggca tcatatggat tcttactgct
     7681 cataaaatgc acttgaagtc tcgctgcaaa tatggtaatt ttgatcattt taaatctatt
//
LOCUS       DAXLDT010000136         7732 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXLDT010000136 DAXLDT010000000
VERSION     DAXLDT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.136"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            217..351
                     /gene="rpmH"
                     /locus_tag="ACF2SP_002419"
     CDS             217..351
                     /gene="rpmH"
                     /locus_tag="ACF2SP_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFD2360193.1"
                     /translation="MKRTFQPSNLKRKRDHGFRLRMSTRAGRLVIKRRRAKGRKRLSA
                     "
     gene            352..696
                     /gene="rnpA"
                     /locus_tag="ACF2SP_002420"
     CDS             352..696
                     /gene="rnpA"
                     /locus_tag="ACF2SP_002420"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFD2360194.1"
                     /translation="MFAFKKAQRLLKKNDFDFVFESAKKITTDDFIFLFRENKLGYAR
                     LGLALSKKMIAKAHDRNRIKRLLRESFRHTNLPAVDIIILARPGLAKKTNLGINTKLN
                     KTWEKLASCYGK"
     gene            663..908
                     /gene="yidD"
                     /locus_tag="ACF2SP_002421"
     CDS             663..908
                     /gene="yidD"
                     /locus_tag="ACF2SP_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFD2360195.1"
                     /translation="MGKISLMLRQIVCLPIKMYQYFISPLITPCCRYYPSCSEYADSA
                     IKHYGVIKGLLMALNRLSRCHPWSKGGYDPLFPNDKN"
     gene            918..2588
                     /gene="yidC"
                     /locus_tag="ACF2SP_002422"
     CDS             918..2588
                     /gene="yidC"
                     /locus_tag="ACF2SP_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFD2360196.1"
                     /translation="MDIRRIVLYMALALIGLSLWNAWQIDYPAKQPVEEKTASQLTSD
                     GHLLPQIIPSNAEQPITLKAEEKASSSKQLIQVKTDVLDVDIDLKNGDIVKGLLLDYP
                     LSVEDKNKPFPLLQNQASQRYVANSSLFILDGQTPQSLDFDFTSEKEYYELKSDQNQL
                     IVTLNGKSEDGLDVKKEFVFTKGSYLIEVNYKIANTGNSLWKGYFNTQLLRSSPKEDK
                     SSIFHIGSYTGASFSNPGKNRYQKVSFSDMSKSNLDVDAKGGWIAMQQHYFLSAWVPN
                     ADSENKFYTLATDKDYTIGAVSQPITVKPKEDKIVGSKLYIGPEITSVLKGISPSLDL
                     TVDYGILWFLSSLLFSLMKAIYTVVGNWGWSIVLVTVLIKLAFYRLSATSYKSMASMR
                     KLQPKLQALRERYGDDKAKISQATMELYKQEKVNPLGGCLPILIQIPVFIALYWVLLE
                     SVELRQAPFIFWINDLASADPYHVLPLIMGATMLIQQKLNPAPADPMQAKVMMFLPIL
                     FTGLFWNFPSGLVLYWIVNNTLSILQQWYITRKYSDEKPAKKVVATAK"
     gene            2589..3929
                     /gene="mnmE"
                     /locus_tag="ACF2SP_002423"
     CDS             2589..3929
                     /gene="mnmE"
                     /locus_tag="ACF2SP_002423"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFD2360197.1"
                     /translation="MSIDTIVAIATPPGRGGVGIVRISGPKAYAIALCLNGNKALQPR
                     LATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDIIEIQAHGSPVVLDLLIKESIAAGA
                     RLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
                     ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSL
                     VIAGRPNAGKSTLINNLAGRDVAIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDS
                     DDLVEKEGIKRAWQELKRADCVLLVVDINNRDQQNSLLNELRLTLPNKIPIITVYNKI
                     DTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAK
                     ALLLTGQSQLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK"
     gene            4225..6063
                     /locus_tag="ACF2SP_002424"
     CDS             4225..6063
                     /locus_tag="ACF2SP_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg3000 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360198.1"
                     /translation="MVYSKQSAAFRRDKQQFFAQIPKDSKLDTKIVTIDDRVNTIDYT
                     FLLHHKQDLEAEFTQMFTVLQQQDNENKEIFWLYCYYCASLLEAFHKAYSQNSKQAEY
                     AKIKQKIKDHLNKVNVQEEESGFIQSLYDSFLGGFRNLISSPLHASKIRDYVAYSNLC
                     RIYWTFCRLTLTNGLSVARDLKIIEKLDAILGTHTDVDKIISTLQAPTGVITYFSVGF
                     FLMRLIIDGGMLLRHTFFPTELEKGAENGCEIHQMDYLPGPASLEAYRKSYILVHEDN
                     QEVSLYYIPRSGKAEKLSIKENNKLKDALLKKMDKDTSSLRLNSTEVEEIITHQTGHV
                     PEVTTAWDRFKHEFYKRHCNFANDFVWATVNLITNYNYLFNISGPAAGMITSVFLGFD
                     VCMALYKCNLAKQEYLIKKAQYLQEIEDYNNPEKFKKMTPEQRLLHIEMLNKQLRELE
                     INYKTQEATFYFVAAAAALLMMGFTASMLVSPPVLVVGCFFACTLAVAMYLSTGAYSK
                     YEEKSLYLEEAQLTGKNLPVALKEYEAARNDFIFTMVKNTVMPIVLITTFAICWPAAI
                     VLTAMYIGYEIFHAYDQHSEAKAAKQLALAAPSEEKDGYMALATPN"
     gene            complement(6060..6860)
                     /gene="legP"
                     /locus_tag="ACF2SP_002425"
     CDS             complement(6060..6860)
                     /gene="legP"
                     /locus_tag="ACF2SP_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegP"
                     /protein_id="HFD2360199.1"
                     /translation="MKTKSWIASLFYSSLCFSSSYAINLNQVTITDPIMGSRTITYEQ
                     VGEFAIVEGDILIGKAENLKNQGAVITPKVGGSRWPNGVIPYEVAENLPFINKLAILQ
                     AIDHWQKNTNIEFVELTSKNRYDYRDYISFIPAEGTTCSSYVGRQGGKQEINLAPRCT
                     TMNTVHEIGHALGMWHEQSRSDRNQYIRIVWENIDEEHKFNFDQHLTDGKDFGEYDYG
                     SIMHYGRFAFSKNGQPTIMPLVDGVEIGQRTHLSEKDIAAIKAMYPET"
     gene            complement(7065..>7732)
                     /locus_tag="ACF2SP_002426"
     CDS             complement(7065..>7732)
                     /locus_tag="ACF2SP_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360200.1"
                     /translation="IIEKTENLSKIPWKSQPEKKNTDLDYQENGEKEAETNPSELFTP
                     MTKKTKSPHSSDRRENKQKSLCSQLRSCKFFVSSVNNAESELNKKQLPFLIRPSRASN
                     NEYYLFTTVCKTSKGIFNHRYGIDRKGNLYSFDKDGTQKVNIMPKGIIATLKKEIAEE
                     TEKIEKVKDIAQITWVPDEYKSSKSSIKGKNRKPEWHLKFFNENGDSAQVSNTTPTLC
                     KNI"
BASE COUNT         2500 a         1302 c         1456 g         2474 t
ORIGIN      
        1 aaataaatgt aagttattaa aaataaattg ttttttaaaa ttatccacaa gaagcagttt
       61 gtatataata aaaataaatt ttaaaaatat ataaagttaa tataattacg acctgtcaat
      121 aaaaaattac acaaaaagta atcagttatt caaattgaca gttgaaaaaa agttatctat
      181 cattgccaac ctcataaatt tagaagcagg gttatcatga aacgcacttt tcaacctagt
      241 aatttaaaac gtaaacgcga tcatggtttt cgtttaagaa tgtctacacg agctggtaga
      301 ttagttataa aaagacgtcg tgctaaaggt cgtaagcgtt tatctgccta agtgtttgca
      361 tttaaaaaag cgcaacggct attaaaaaaa aatgattttg atttcgtgtt tgaaagcgcg
      421 aaaaaaataa ctacagatga ctttattttc cttttcagag aaaataagtt aggctatgca
      481 cgccttggtt tagcattgtc aaaaaaaatg atagctaagg ctcatgacag aaatcgtatt
      541 aaacggctac ttagagaaag ttttcgtcat actaacttgc ctgctgtaga tataatcatt
      601 ttggcaagac caggtctagc aaagaaaacc aatttaggta taaatactaa attaaataaa
      661 acatgggaaa aattagcctc atgctacggc aaatagtgtg cttaccaata aaaatgtatc
      721 agtattttat tagccctttg ataacaccat gttgtcgcta ttatccgagt tgttcagagt
      781 atgctgacag tgcgatcaaa cattatggcg taattaaagg tttattgatg gcacttaaca
      841 gattgtctcg ttgccatcca tggtcaaaag gtggctatga tcctttattt cctaacgata
      901 agaattgaga atatttaatg gatatacgac gtatagtttt atatatggcg cttgcgctta
      961 ttggtttatc actttggaac gcttggcaaa ttgattatcc agccaaacaa ccagttgaag
     1021 agaagacggc aagccaatta acatcagatg ggcatttgtt accacaaata ataccttcta
     1081 atgcagagca acccataact ttgaaagcag aggaaaaagc aagcagcagt aaacaattga
     1141 ttcaggttaa aacagatgtt ttggatgtcg acattgattt aaaaaatgga gatattgtta
     1201 aaggtttatt gcttgattat ccactaagtg ttgaagataa aaataaacca ttccctctct
     1261 tacaaaatca agccagccag agatatgtag cgaacagtag cctatttatt ttagatggcc
     1321 agacaccaca atcattggat tttgatttta catcagaaaa agaatattac gaattgaaat
     1381 ctgatcaaaa tcagctgata gtcactctaa atggcaaaag cgaagacggt ttggatgtga
     1441 aaaaagaatt tgtttttaca aaaggaagtt atttaatcga agtcaattac aagattgcca
     1501 ataccggtaa ttcattatgg aaagggtatt ttaataccca gttattgcgc agttctccta
     1561 aagaagacaa atcgagcatt tttcatattg gctcgtatac aggtgcttcc ttttcaaatc
     1621 ctggaaaaaa tcgttaccaa aaagttagtt tcagtgacat gagcaaatct aacctggatg
     1681 ttgatgcaaa aggtggttgg attgctatgc aacaacacta ttttttgagt gcttgggttc
     1741 ccaatgcgga tagtgaaaac aagttttata cgttagctac ggataaagat tatactattg
     1801 gagcggtaag ccagcctatt acggtcaaac caaaagaaga taagattgta ggatccaaat
     1861 tatacatagg gccagaaatt accagtgttt taaaaggcat atccccatct ctggatttaa
     1921 cggtggacta tggcattttg tggttcttgt cttcgttatt attttcttta atgaaagcaa
     1981 tttatactgt tgttgggaat tggggctggt caattgttct tgtgactgtc ctaattaaat
     2041 tggcatttta tcgattatca gctacaagct ataaatccat ggctagtatg cggaagttgc
     2101 agcctaagtt gcaagctttg cgtgagcgat atggtgatga taaagctaaa ataagccaag
     2161 ctaccatgga actttataag caagaaaaag taaatccttt aggtggttgt ttacctatat
     2221 taattcaaat tccagttttt atagcgctct attgggtttt actggaaagt gttgagctaa
     2281 gacaagctcc atttatattt tggataaatg atttggcttc agcggatcct taccatgtcc
     2341 ttccattaat catgggagca accatgttga ttcagcaaaa gctcaatcca gcaccagctg
     2401 atccaatgca agctaaggta atgatgtttt tacccatatt atttaccgga cttttctgga
     2461 attttccttc tggtctggtt ttgtactgga ttgtgaataa tactttgtca atattacagc
     2521 aatggtacat caccagaaaa tactcagatg aaaagccagc taaaaaagtt gttgccacag
     2581 caaaataaat gtcaatagat accattgttg ctatcgctac cccgccagga cgaggcgggg
     2641 taggaatcgt cagaatatca ggtccaaaag cttatgctat agccctttgt ctaaatggga
     2701 acaaagcatt gcaaccacgc ttagccactt tttgctcttt atataaagga aataatgaag
     2761 tccttgatca aggtttggtt ctttatttta aaggaccaca ttcttttaca ggcgaggata
     2821 ttattgaaat tcaagcgcat ggctcaccag tagttttgga tttattaatt aaggaatcaa
     2881 tagcagcagg ggcaaggcta gccaggccgg gtgagttttc tgaaagagca tttcttaatg
     2941 ataaaattga tttaattcaa gcagaagcca tagctgattt aattcaagcc agttcagata
     3001 ctgcggctcg tatggcttta aaatcactgc aaggtgattt ttctaaaaaa ataaatcaat
     3061 tgaatgaaga gctaatttac ttacgcatgt atgttgaagc cgctatcgat tttcctgaag
     3121 aggaaattga ttttcttaat gatggaaatg tcagtcaatt actacaaaga attattgggc
     3181 gattagaaga gatacgttca caagcgaatc aaggagtttt gttgcgtgaa ggattatcct
     3241 tggttattgc tggcaggcct aatgcaggaa aatcaacctt aattaacaac ctggcagggc
     3301 gagatgttgc cattgttact gaaattgctg gtactacgcg agatattatg cgagaacata
     3361 tcctattgga tgatatccct ctacacatta tcgatacggc aggattaaga gacagcgatg
     3421 atctggtgga gaaagaggga ataaaacgtg cttggcaaga gttgaagcga gctgattgtg
     3481 tgcttcttgt agtagatatt aacaatcgtg atcaacaaaa ttctctgctt aatgaattga
     3541 gattaacatt acccaataaa atacccataa tcacggtata caataaaatt gatactacta
     3601 aacttacagc aaaatgtgat gagcatacgg tttatttgtc tgcaaaaact ggtgaaggac
     3661 tggatgagct aaaaaaagtg atcaaacaag tggttggata tcaaccaaca gaaggccaat
     3721 ttttagctcg cagacgacat ctacaagcgt tggacgaagc aaaagctttg ttactaaccg
     3781 ggcaatcgca gctaacaaat cataaagctg gagaattgtt ggcagaagat ttacggttag
     3841 ctcatcagac tttatgtgaa attactggtg agttcacttc agacgattta ttaggaaaaa
     3901 ttttttccag tttttgtatt ggaaaataac gatgatgcaa ggcagccatc atgtgaattt
     3961 tcaatttttc ttcttttgaa tacagtaaaa ttattattaa atattctgtg gtgatgtgct
     4021 ttattagatc aataaattta ttcttttagt ttcaacacat gtcaaacgaa ccggctttta
     4081 tcttgattca attttatgca aaaatcagtt tgaatcgcaa aaatcaatgt gtaaaatatt
     4141 aatacaaaaa agcttgtcaa aatacatatc aaatggtaac gtattattgt aaaacttcct
     4201 gtgtaatttt tgtgagttct taatatggtc tactcaaaac aaagtgctgc atttcgtagg
     4261 gataaacaac aatttttcgc ccaaattccg aaggatagta aactggacac aaaaattgta
     4321 acaattgatg atcgagtaaa cactatagat tacacttttt tactacacca caaacaggat
     4381 ttagaagctg aatttactca aatgtttact gtgttacagc agcaagacaa tgaaaataaa
     4441 gaaattttct ggctgtattg ctactattgt gcgtccttgc tggaggcttt ccacaaagct
     4501 tattcacaaa acagtaaaca agctgaatac gctaaaataa aacaaaaaat aaaagaccat
     4561 ttaaataagg tcaatgtcca agaggaagaa tctggtttta ttcaatcctt gtacgattcc
     4621 tttcttggag gttttcgcaa tttaatcagc tctccattgc acgcttccaa aatcagagat
     4681 tatgttgcct actcgaactt gtgtcgtatc tattggacgt tttgtcgttt gactttaact
     4741 aatggattga gcgtagctcg agacctaaaa attatcgaga agttggatgc tattttagga
     4801 actcatactg atgttgataa gattatttca actcttcaag cacctaccgg ggttataact
     4861 tactttagtg tcggtttctt tttgatgcgc ttaataattg atggcggtat gctattaaga
     4921 catacctttt tccctactga attggaaaag ggcgcagaaa atggttgtga aatccatcaa
     4981 atggattatt tgccaggccc agccagtctc gaagcatata gaaaaagtta tattcttgtt
     5041 catgaagaca atcaagaagt atcactctat tacattccta gaagtggtaa agcagaaaaa
     5101 ttaagcatta aagaaaataa caaactgaaa gatgctttat tgaagaaaat ggataaggac
     5161 acatcatccc ttcgtttgaa ttcaactgaa gtagaagaga taatcactca tcaaacaggg
     5221 catgttcccg aagtgactac cgcatgggac agatttaaac atgagtttta taaacgacac
     5281 tgtaattttg ccaatgattt tgtttgggct acggtcaacc tgattaccaa ctataattac
     5341 ctttttaata tttccggtcc tgcagcaggg atgatcactt ctgtttttct cgggttcgat
     5401 gtatgcatgg ctttatataa gtgcaactta gccaaacaag agtatttgat caagaaagct
     5461 caatatttgc aggaaataga agactataat aatccggaaa aattcaaaaa aatgactcct
     5521 gaacagcgat tattacatat agagatgctc aataagcaac ttagagagct tgaaattaat
     5581 tataaaaccc aggaggctac tttctacttt gttgccgctg ctgccgcctt attgatgatg
     5641 ggatttactg catcgatgct ggttagtccg cctgtattag tcgttggttg tttttttgcc
     5701 tgtactcttg ctgttgcaat gtatctgtct acaggagctt attcgaaata cgaggaaaaa
     5761 agtttgtatt tggaagaggc tcaattgaca ggtaaaaacc ttccagttgc tcttaaagaa
     5821 tatgaagcag ctcggaatga ttttattttt accatggtaa aaaatacggt catgcctata
     5881 gttttaatta caacatttgc aatttgctgg cctgctgcaa ttgttttaac agctatgtac
     5941 attggttatg aaattttcca tgcttatgac caacactctg aagcgaaagc ggccaaacag
     6001 ttggctttag ccgccccttc agaggaaaaa gatggatata tggccttagc cactcccaat
     6061 taggtttcag ggtacattgc tttgatggca gcgatatctt tttcacttaa atgagttcgc
     6121 tgccctattt caactccatc aactagaggc atgatggttg gctgaccatt cttggaaaaa
     6181 gcaaatcgac cataatgcat aatggatccg tagtcgtatt caccgaaatc tttcccatca
     6241 gttaaatgtt ggtcaaaatt aaatttatgc tcctcatcaa tattttccca gacaatacga
     6301 atatattgat ttctgtcaga tcgggattgc tcatgccaca ttcccaaggc atgtcctatt
     6361 tcatgtaccg tattcatggt agtacatctt ggcgccaaat tgatctcttg cttccctccc
     6421 tggcgtccaa cataggaaga gcatgttgtg ccttctgccg ggataaaaga aatgtaatca
     6481 cgatagtcat aacgattttt agaagttaat tcaacaaatt ctatattcgt gtttttctgc
     6541 cagtgatcta ttgcttgtaa aatggcgagc ttgtttataa aaggtaaatt ttcagcaact
     6601 tcataaggga taacaccatt tggccagcgt gaacctccta cttttggtgt aataacagca
     6661 ccttggtttt taagattctc tgctttacca atcaaaatat caccttctac tattgcaaat
     6721 tctccaactt gttcataggt aatggtacga ctgcccatta ttggatcagt tattgtgact
     6781 tgattgagat taattgcgta agaagaggag aaacataatg aagaataaaa caaactggca
     6841 atccatgatt tagttttcat atatagtcct taagtctaaa aaaaccaagg acagtttagg
     6901 ggaaagaggg agtagatttc cagaactttt tatatagttt gttgactctt gataacctta
     6961 aagcgaggac gcttttacaa aacgtcctta attgataatt atgtgcgatt acatgaataa
     7021 atagctataa gaatatagcg atgcgaaagt atataaggct aagtttaaat atttttgcaa
     7081 agagtcggtg tagtgttgct aacttgagca ctatctccat tttcattaaa gaactttaaa
     7141 tgccactctg gttttctatt ttttcctttt attgatgatt tagaggattt atattcgtca
     7201 ggcacccaag ttatttgagc aatatcctta actttctcaa ttttctcagt ttcttctgca
     7261 atctcctttt tcagagtcgc tatgatacct tttggcataa tatttacttt ttgtgttccg
     7321 tctttatcaa aactatagag attacctttt ctgtctattc cgtatcgatg attaaaaatt
     7381 ccctttgaag ttttgcagac cgttgtaaac aggtaatatt cattgttact ggctctgctg
     7441 ggacgtatta gaaaaggtaa ttgtttttta ttcaattcac tttctgcatt gttaacgctt
     7501 gagacaaaga atttgcagga acgcaattga gagcataagc ttttttgttt attttctctt
     7561 ctatccgaag agtgagggga ctttgttttt ttagtcattg gagtaaataa ttcagaggga
     7621 tttgtttcag cttctttttc cccattttct tgataatcaa ggtcggtatt ttttttctca
     7681 ggttgggatt tccatggaat tttgctgaga ttctccgttt tttcaataat ca
//
LOCUS       DAXLDT010000137         7691 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXLDT010000137 DAXLDT010000000
VERSION     DAXLDT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.137"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(221..1465)
                     /locus_tag="ACF2SP_002427"
     CDS             complement(221..1465)
                     /locus_tag="ACF2SP_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL and HDOD domain-containing protein"
                     /protein_id="HFD2360201.1"
                     /translation="MVVELDEPPVRTLLARQGIYDKNSAIFAYELLYRNSEAQNSQVD
                     NLNPSSGEAATSSVLLQLFANLDVNTIIGNKQAFINFTHNHLVQKIPMLLPKNRIVIE
                     VLETVAIDQALLLNLMELKKQGYQIALDDFVFRNELAPLVDMADIIKIDVLNLDQQQI
                     AEQLLPLKSFRGKLLAEKIEDKQQFNHCVNLGFDFFQGFFLNKPDSLTGQIITENKIQ
                     LLRLLTEINNEEVPIQRIEEIILQIPKLSYRILRLANSASLYMSKKIESLMDAISQLG
                     LMQIRNWLNLLLLASLDDVAPDLLERTLIRAKMCESLAKSMDYPNSHQAYTVGILSTL
                     DGILNEPMPSLLAKIRLSEALNEALLNYKGDLGKILKLAIDYEQANFNQLERIPVKSE
                     TLSQSYLKGIEYANHVIDIINK"
     gene            complement(1670..3124)
                     /gene="gcvPB"
                     /locus_tag="ACF2SP_002428"
     CDS             complement(1670..3124)
                     /gene="gcvPB"
                     /locus_tag="ACF2SP_002428"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFD2360202.1"
                     /translation="MLIFELSKTGRQAKAQIPRAVSKNYSIPEEFQRKSPPRLPACSE
                     LQVVRHFTCLSQKNFSIDTNFYPLGSCTMKYNPRGVHKAASLPGFINRHPLAMDNESQ
                     GFLETLYKLQNYISEITGMPGVSLTPMAGSQGEFAGVAMIKAYHQSRGDTARDEILIP
                     DAAHGTNPASAVMCGFKVVEIATAPDGDIDLDELKRKVGPRTAGIMLTNPSTLGLFMR
                     QIKEIANLVHQAGGLLYYDGANLNAILGKVRPGDMGFDVMHLNLHKTFATPHGGGGPG
                     AGPVAVGKRLIPYMPLPVVKKTDSGYHWATRQDYPQSIGRLSCFMGNAGILLRAYFYM
                     LVLGKEGLLRVSEFATLNANYLLKELTKVGYTAAYPGRRASHEFILTLNSEKKNYDVT
                     AMDFAKRLLDYGVHAPTTYFPLLVPECLLIEPPETESKEELDAFVAVMKTIREEAIKQ
                     PDILKAAPHTLPVKRLDDVKAARELDLNYFATHE"
     gene            complement(3118..3426)
                     /locus_tag="ACF2SP_002429"
     CDS             complement(3118..3426)
                     /locus_tag="ACF2SP_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFD2360203.1"
                     /translation="MFIVQLTYLVPINEVNKYLQAHREFLDYHYKQGLLVASGPMKPP
                     TGGIIIAATNDRAHLESVFKQDPYYLAEIAEYQFIEFTPVKHRDELKELIQKTEGKLC
                     "
     gene            complement(3426..4796)
                     /gene="gcvPA"
                     /locus_tag="ACF2SP_002430"
     CDS             complement(3426..4796)
                     /gene="gcvPA"
                     /locus_tag="ACF2SP_002430"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFD2360204.1"
                     /translation="MPYIPHTPNDTKEMLTAVGAQDIQDLFDEIPASLQYAGFQSIPA
                     GINEMEMLKEAQNQAQKNRNGICFIGAGCYEHHIPAAVWDIASRGEFLTAYTPYQAEA
                     SQGTLQLLYEYQTMICELTGMEVSNASMYDGATALAEAVLMAVRLNKHSKTNRVLITG
                     TVHPFYRETIETIVRNQHIEVITLPFDEQQGITDFGSLNQYTGEDITALVIAQPNFFG
                     CLEQVDKMTSWAHHNKTISVACVNPTSLALLKPPCSWGEHGVEIVCGEGQPLGSPMAS
                     GGPYFGFLSTRMAHVRQMPGRIIGRTVDKDGKTGFSLTLQAREQHIRRAKATSNICTN
                     QGLLVTAATIYMSLLGPEGLSQVATQCHQNTHELITALTQIEGVELAFKAPFFHEALI
                     KLNQPVQSVLQQLADAGIAGGYAPEQHYPQLANTLLVCATEVRSAEDIAKYAKTLKTI
                     MSKRGA"
     gene            complement(4799..5176)
                     /gene="gcvH"
                     /locus_tag="ACF2SP_002431"
     CDS             complement(4799..5176)
                     /gene="gcvH"
                     /locus_tag="ACF2SP_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFD2360205.1"
                     /translation="MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIG
                     DEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKP
                     SHPDEIKSLLSDEQYQNEIAEEN"
     gene            complement(5210..6292)
                     /gene="gcvT"
                     /locus_tag="ACF2SP_002432"
     CDS             complement(5210..6292)
                     /gene="gcvT"
                     /locus_tag="ACF2SP_002432"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFD2360206.1"
                     /translation="MTAKTPLHTTHLACGAKMVDFHGWDMPLHYGSQLNEHHAVRNDA
                     GMFDVSHMTIVDILGAGGRQFLRKLLTNDVDQITHNGKALYSCMCNEHGGIIDDLIVY
                     QRASDNYRVVLNSATRQNDVAWIRAKSEGFAVGLQERRELSMLAVQGPNAIAKTLSIL
                     APAHVDAVSTLTSFECVDVDHWFFARTGYTGEDGLEIIVPNEFVTQLWNDLLNAGVTP
                     CGLGARDTLRLEAGMLLYGQDMDETTTPLESGLAWTVKWEPEDRGFIGMGALASQKQQ
                     GIKRKMVGLTLLDKGIMRHGQKVIIEGCPDGIITSGSYSPTLQQSIALARVPVETGEQ
                     VLVDIRGKLIPAKVGKPRFIKQGKPV"
     gene            6427..6894
                     /locus_tag="ACF2SP_002433"
     CDS             6427..6894
                     /locus_tag="ACF2SP_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFD2360207.1"
                     /translation="MVWPIIWRWILDLGWLCFLLILFWYFWKKRRDLVEARSWLKAKG
                     HVTRCEWTRVGHSVWPRIEYIYEVYEKDLTGEYLFLDTTFNTPNSKYSRSIAYKVAIA
                     YRDNSEIDVYYNPNRPEQSALDVTIPKKLTFILILISSLILLHIGIIVWRLLV"
     gene            complement(6869..7399)
                     /locus_tag="ACF2SP_002434"
     CDS             complement(6869..7399)
                     /locus_tag="ACF2SP_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFD2360208.1"
                     /translation="MNKTITIALSLLLSIASKLTYAGPDRAQLAVWANEAIIATYTFD
                     YKNYMQQQKEIAKYFSADGWIAYSKALNQSKLPEVVQKNAYFVNAVATEPPKLITLDP
                     THWQAIMPILVVYKNPQYEQKQNLKVILGFTVASPGQGVRGFSVTSLQSTPISPPCQC
                     KIEETPGNTKQGDAKQ"
BASE COUNT         2148 a         1510 c         1585 g         2448 t
ORIGIN      
        1 ataaatttaa cattctatga attataaata aacaaaggga aacatcaagc tattatttga
       61 tcgataagga caatttaagg tctttgtgat tttaaataaa atttagtgag ttattcgtgg
      121 tatgaaaatc ccttttgtac tatctgatat ggacaatttc catagtattg tcttttgtaa
      181 aacaggttga atacagaatg aatactaata gggacgacaa ttatttatta ataatgtcta
      241 tcacatgatt ggcgtattct attcccttaa gataagattg agagagcgtt tcgcttttta
      301 caggaatgcg ttctaattga ttaaaatttg cttgttcata atctatggca agtttcagga
      361 ttttgcctaa atccccttta tagttcagta gcgcttcatt gagtgcttca cttaaccgaa
      421 tttttgctaa tagcgaaggc ataggctcat ttaaaatacc atctaaagta gaaagtatgc
      481 ctacggtata agcttgatga gagttaggat aatccattga ttttgctaag gactcacaca
      541 ttttggcgcg aattaaagtt cgctccaaca aatcaggtgc gacatcatcg agacttgcca
      601 agagtaacag atttaaccaa ttgcgtattt gcattaatcc caattgagaa atggcatcca
      661 ttaatgattc tatttttttg ctcatgtaca aagaggcaga atttgccaaa cgcaagatac
      721 gatagctaag tttgggaatt tgcaggataa tttcttcaat gcgttggatt ggcacctctt
      781 cattgtttat ttctgttaat aatcttaaaa gctgtatttt attttcagta atgatttgtc
      841 ctgttaagga atccggtttg tttagaaaaa aaccttgaaa aaaatcaaat ccaagattga
      901 cgcaatgatt gaattgttgt ttgtcctcaa ttttttctgc cagcaactta cctctaaaag
      961 atttcagcgg taataattgt tctgcgattt gctgctggtc aagattaagc acatcgattt
     1021 taataatgtc agccatgtca accagaggag ctaattcatt cctgaagaca aaatcatcca
     1081 gggcaatttg atatccctgt ttctttaatt ccattaaatt gagcagtaat gcttggtcaa
     1141 tcgctaccgt ttcaagaact tcaatgacga ttctattttt aggaagcagc attgggattt
     1201 tttgtacaag atggttgtgg gtgaaattaa tgaaggcttg tttattgcct attatggtat
     1261 ttacatcaag attggcaaat aactgcaata gaacagacga ggtagctgct tcacctgacg
     1321 acggatttag gttatctacc tgcgagtttt gtgcttcact attacgatat aagagttcat
     1381 aggcaaagat tgcgctgttc ttatcatata ttccctggcg cgctagcaat gttctgactg
     1441 gtggctcatc caattctaca accatttatt tgatccatta gaatgacatt ataacattat
     1501 tttataaagt taataatacg atgcttgaga gccttggaat gcagaagttt gtgaattgtt
     1561 ataaaagagc atcagacttt tggtgagaga agattggttg tcataaaaca cagcaactgt
     1621 catcaaaaga aagctgctgt gctaaaattg gaaaaatctg ttgtatagat tattcgtgag
     1681 ttgcaaaata atttaaatcc agttcacgag ccgctttcac atcatccagt cttttgactg
     1741 gtaaagtgtg gggcgcggct ttgagtatat caggttgttt gatagcttct tcgcgaatag
     1801 ttttcatcac ggctacaaaa gcatccaatt cctctttgct ttctgtttca ggtggttcaa
     1861 tcaacaagca ttccggtacc agtaaaggaa aataagtagt gggggcatga accccatagt
     1921 ccaataacct ttttgcaaaa tccatggcag tcacgtcata atttttcttt tcagaattta
     1981 aagtaagaat aaattcatgt gatgcacgtc tgccaggata ggctgctgta tagcccactt
     2041 tagtcaattc tttaagtaaa tagttggcat taagtgttgc gaactctgat acccgtaata
     2101 ggccttcttt accaaggaca agcatataaa aataagcacg taataaaatg ccggcattac
     2161 ccataaagca cgataatctt cctatgcttt gtggataatc ctggcgagtt gcccagtgat
     2221 atccggagtc ggtttttttc acaacaggta agggcatgta aggaataaga cgtttgccaa
     2281 cagcaacagg accagcgcca ggaccgccac ccccgtgagg tgtagcaaat gttttatgta
     2341 aattaagatg catgacatca aaacccatat caccaggtct tactttacct aaaatagcat
     2401 tgagattagc cccatcgtag tatagtaacc ctccggcctg gtgtacaagg tttgctattt
     2461 cttttatctg acgcataaat aatcccaaag tggatggatt agttagcata attccagcag
     2521 tcctggggcc tacttttctt tttaattcgt caagatcaat gtcaccgtcc ggagcagtag
     2581 ctatttctac tactttgaag ccacacatta ccgcagaggc gggatttgtt ccatgcgccg
     2641 catctggaat gagaatttca tctcgggcgg tgtcaccacg agactggtgg taggctttaa
     2701 tcatggcgac tccggcaaat tctccctgtg aacctgccat tggagttagt gaaacaccgg
     2761 gcattcccgt aatttcagaa atataatttt gtaatttata aagagtttcc agaaatccct
     2821 ggctttcatt gtccatggct aacggatgtc gatttataaa accgggaagc gaagctgctt
     2881 tatgaacacc ccggggattg tatttcatgg tacatgagcc caaaggataa aaattggtat
     2941 ctatggaaaa attcttttgg gaaaggcagg taaaatgtct taccacttgc aattcggaac
     3001 aggccggcaa tcttgggggc gactttcgtt gaaattcttc gggtattgag tagtttttgc
     3061 ttactgctct gggtatctgg gcttttgcct ggcgaccagt tttagataat tcaaaaatca
     3121 acataatttg ccttccgttt tttgaataag ttccttgagt tcatccctgt gttttactgg
     3181 agtaaactcg ataaattgat actcagcaat ttctgctaaa taataagggt cttgtttaaa
     3241 aactgactct aaatgggccc tgtcatttgt cgctgctata ataatcccgc cggttggagg
     3301 tttcatgggg ccagaagcaa ccaatagccc ttgtttatag tggtaatcaa gaaactccct
     3361 gtgagcctgc agatatttgt ttacttcatt gatgggaact aaataggtta attgcacaat
     3421 aaacatcagg cacctcgctt ggacattata gtttttaatg tttttgcata tttcgcgata
     3481 tcctctgcag agcgtacttc tgtcgcacaa actaacagcg tattggctag ctgagggtag
     3541 tgttgttctg gtgcgtaacc tccagcaatt ccagcgtcgg ccagttgttg caatacggac
     3601 tgaactggtt ggtttagttt aatcaaggct tcatggaaaa acggagcttt gaatgccagt
     3661 tctaccccct cgatttgtgt tagggcagta atcaattcat gagtgttttg atggcattga
     3721 gtcgccactt ggcttaaacc ttccggccct aaaagactca tatagatagt tgctgctgtt
     3781 acaagtaaac cttggttggt gcatatgttt gaagtggctt tagcacgccg aatatgctgt
     3841 tctcttgctt ggagagttaa actaaaaccg gtttttccat ccttgtctac cgtgcggcca
     3901 attattcttc caggcatttg tctgacatgg gccatacggg tgctcagaaa accaaaataa
     3961 gggcctcctg atgccatagg cgagcctaaa ggttgccctt cgccgcatac aatttctaca
     4021 ccatgctctc cccatgagca gggtggtttt aataaagcca atgaagtcgg gttaacacaa
     4081 gctacactga ttgttttgtt atgatgtgcc caggaagtca ttttatcgac ttgctcgagg
     4141 caaccaaaga aattcggttg ggctatgacc aaagctgtaa tatcttctcc agtgtattga
     4201 ttgagagagc caaaatcagt gattccttgc tgttcatcaa aagggagggt gatgacttca
     4261 atatgctgat tacgtactat cgtttcaatg gtttcgcgat aaaaagggtg aactgtgcct
     4321 gtaatgagta ccctgttggt tttgctgtgt ttattaagcc tgactgccat tagtactgct
     4381 tcagctaacg cagttgctcc gtcatacatt gacgcattgg atacttccat gccagtcaac
     4441 tcacagatca tggtttgata ttcatataat aattgcaaag ttccctggct ggcctccgct
     4501 tgatagggtg tgtaagcagt taaaaattca ccgcgagacg caatatccca cacggctgct
     4561 ggaatatgat gctcataaca tccagcacca ataaaacata tgccgttgcg attcttttgg
     4621 gcttgattct gggcttcttt caacatttcc atttcgttaa tgccagcagg gatactctga
     4681 aatcctgcat attgcaaaga agctggtatt tcatcaaata aatcctgaat gtcttgagca
     4741 ccaactgccg ttagcatctc tttggtatca ttgggcgtgt gtggaatata aggcataatt
     4801 aattttcctc agctatctca ttttgatatt gctcatcact caacaggctt ttgatttcat
     4861 caggatggct gggctttagc ttcacgagcc aaccttcatg gtagggatcg tggtttacca
     4921 gggcagggtt tttaccaacg gcttcgttaa ccgccgttac aacaccacta atgggagcat
     4981 aaaaatcaga agctgctttc actgactcca ccacgcctaa ttcttggcct gcactcactt
     5041 catcacctat ttcgggtagt tctacaaata ccatatcccc caataattct tgcgcatgat
     5101 ctgtaattcc tactgtgacc tcttcctcat cttctctcag ccattcatgg gttgttgtga
     5161 atttcaaatc attcattttg tatatcctta atattaatat tactaaaatt taaacaggtt
     5221 ttccttgttt gataaagcga ggcttcccta cttttgccgg gattaacttt ccgcgtatgt
     5281 caacaagtac ttgttcccct gtttccacgg gaactcgtgc caaagctata gattgttgca
     5341 aggtaggaga ataactgccg cttgtgataa ttccatcagg gcagccttca ataataactt
     5401 tttggccatg acgcataatg cctttgtcca acaaggttaa accgaccatt ttacgtttaa
     5461 ttccttgttg cttttgagaa gccaaagcgc ccattccgat aaatcctcta tcttccggct
     5521 cccactttac tgtccaggcc aaaccagatt ccagtggggt tgttgtttca tccatatcct
     5581 ggccgtaaag aagcattcct gcctctaatc tcagagtatc tctggcccct aatccacatg
     5641 gcgttactcc agcattcagg agatcattcc agagttgagt aacaaattca ttagggacaa
     5701 tgatttcaag gccatcttcc cccgtatagc cggtgcgagc aaaaaaccag tgatccacat
     5761 caacgcattc gaaagaggta agagttgaaa cggcatctac atgggcgggt gcaagaatac
     5821 tcaatgtttt cgctatggca tttggacctt gtacagcgag cattgataat tcgcgccttt
     5881 cttgtaagcc aacagcaaat ccttcacttt ttgcgcgaat ccaggccaca tcattttgcc
     5941 tggttgccga attaagtacc actcggtaat tatcggaagc gcgttgatat acaataaggt
     6001 catctataat tccgccgtgt tcattacaca tacagctgta tagggctttt ccattatgag
     6061 taatttgatc gacatcgttg gttagcagtt tgcgcagaaa ttgacgacca ccggcgccta
     6121 aaatatcaac gatggtcata tgtgagacat caaacatgcc agcatcgttt ctgacagcat
     6181 gatgttcgtt taactgagat ccataatgaa gaggcatatc ccaaccatgg aaatccacca
     6241 ttttggcacc acatgctaag tgcgtagtgt gaagaggagt tttagcagtc attgcatttc
     6301 ctttttttat cacttattgg atgtgattat acctgttggg attaattttg cgaggtttcc
     6361 aatttgattt ttttgcatga tcctctccct ggaaatagca tataatataa atttatggga
     6421 aagaatatgg tgtggccgat aatttggaga tggatccttg atttaggatg gctgtgtttt
     6481 cttttaattc ttttttggta tttttggaaa aaaagaagag atttggttga agcaagatcc
     6541 tggttaaaag cgaaggggca tgtcacgcgc tgtgagtgga caagagtagg gcatagtgtt
     6601 tggcctagaa tagaatatat ctatgaagtt tatgagaagg atttgacagg ggaatatttg
     6661 tttttggata caacatttaa tacacccaac agtaaatatt cccgaagtat agcatataaa
     6721 gtggcaatag cttacagaga taactcagaa attgacgtct actataatcc caatcgtcct
     6781 gaacaatctg ctttagatgt taccatacct aaaaaactaa cttttatttt aattctaatc
     6841 agctcattaa ttttgctgca tattggtatt attgtttggc gtctccttgt ttagtgttcc
     6901 caggcgtctc ttcaatttta cactggcatg gcgggctgat gggggtggat tgcaggctgg
     6961 ttacgctgaa acctcttaca ccttgtccgg gagatgcaac ggtaaaccct aatataactt
     7021 ttaagttttg tttttgctcg tattgggggt ttttatatac gaccagtatg ggcatgatag
     7081 cttgccagtg tgtcggatcc agggtgatca gcttgggtgg ttctgtggca acagcattga
     7141 cgaaataggc atttttttgt actacttcag gcagttttga ttgattaagc gctttgctat
     7201 aggctatcca gccatcagct gaaaaatatt tggctatttc cttttgttgt tgcatgtaat
     7261 ttttataatc aaaagtgtaa gtggcaataa tcgcttcatt agcccaaact gccaattgtg
     7321 ctctatctgg ccctgcataa gttagttttg aggcaatact tagaagtaaa ctcaaggcaa
     7381 tagtgattgt tttgttcata ataaattctc atcgattata tcaatactgc cagtttagtc
     7441 attttttcat tacaaaaaaa tccccggcta agatcccggg gagaattata tatccggatt
     7501 tggtgcaatc cggatactac cccgcataaa gagggggaaa gcggggtaga gcactcgggg
     7561 gagaatgctc actatattag acacatgaat tgcaaaaaag ttcaaaattt aatcaaaatt
     7621 atttgtaaat acggttgaat gtgttggttt gtctggtatc gatgacgttg tatttatggt
     7681 attttatact c
//
LOCUS       DAXLDT010000138         7676 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXLDT010000138 DAXLDT010000000
VERSION     DAXLDT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.138"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1360
                     /locus_tag="ACF2SP_002435"
     CDS             <1..1360
                     /locus_tag="ACF2SP_002435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360209.1"
                     /translation="VIAAYNVLLFNWVRGVGRFKMPKLSEMNSVTVWFEPGVGSGHIN
                     ATIARMNELCLLGFRGSFNIIYLSNMKLSGPKEQMELIARGESCTDKYSLSYGQVLSS
                     GEYTPPEIIERLLVEKFPHRIKQFLLNDDEKLSIPPGTHDELCISGAADQTSEAPVNP
                     NEVSLLRVQMDMAFGCSVMIQLQPPRWLAGVSYVCRSGSARETVSSLPHKIDDEIKQG
                     FRSIGAIGARPNVVKIGCYGIRNITSPGVISTLVTLSKAKERCEILEIGSEPSVADST
                     KKGILGKTDLGKFYAEIDLMICEGQNTATECLALGVPVLHLPKPHAGNIYPSDRELRD
                     LGFNMPEHKKVYDSVHSMIALAQDFTTGSGNLCSSLQKIREHADRGALAQLADKINAR
                     PSVLEDILTRLGIQLAIKAAAPEVVQQVDMNPTKQVKEALGAAKKLDSNFQDTGFKTL
                     LP"
     gene            1730..3376
                     /gene="mdcA"
                     /locus_tag="ACF2SP_002436"
     CDS             1730..3376
                     /gene="mdcA"
                     /locus_tag="ACF2SP_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFD2360210.1"
                     /translation="MDWNKNLKDYQSRLISIKPFFKKGKIVSEKNIVALLETAIKPHD
                     KVCLEGDNQKQADFLARSLCSVNPQKIYGLHMLQSAVTLSEHLDLFERGIAEKIDFAF
                     AGPQSTRLAIMIQRNQVKIGNIHTYNELYARYVMDLTPNVALITAEKADYYGNLYTGA
                     NTEETPSIVEATAFKNGLVIVQANEIVKKLPRIDIPGEWVDAIVQAPSPSYIEPLFTR
                     DPALIDEVKILMAMMVIKGIYAPYQVNRLNHGVGFNTCAIELILPTYAQSLGLKGKIC
                     KHWMVNPLPTLIPAIEAGFIESIFCVGGEVGMNEFIRSKPDIFFTGRDGSLRSNRLYG
                     QLAGHYGCDVFIGATLQMDPQANSSTATKGRIAGFGGAPNMGCDTLGRRHSSYAWLKA
                     GQEKNSHNPVAMPRGRKLVIQMVETFQGAGKPTFVEKLDAWELQKEMQADLPPIMIYG
                     DDISHVVTEEGIANLLLCKNSEEREQAIRGIAGYTPVGLKRDKAIVDELRHRGIIQRP
                     EDLNISLKEADRDLLAAKNIHDLVEISNGLYCPPNKFRNW"
     gene            3385..4581
                     /gene="mdcD"
                     /locus_tag="ACF2SP_002437"
     CDS             3385..4581
                     /gene="mdcD"
                     /locus_tag="ACF2SP_002437"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFD2360211.1"
                     /translation="MDTYQYTFSLPKKLQISPMIKVGVAGSGNLEVIIKPNSNCDKTD
                     IIVNTVISGFRNTWDAVIVRFVEDYPYGGLSITLNDAGATPPVVSLRLRQAIETYQTG
                     YPKKDSYTEANARTRIYSLVDEASFTEFLLDKETPSPTLPQLNMQVETDDGVIIGIAK
                     MNGIDIAIVSQQKDFIGGSVGEIHGAKINGLIKYAIKNQLPAIIFLIDSGGVRLQEAN
                     VGEIEISEIIRSILDARSAGIKTIGVICGNNGAFGGMGIISGTLDYLIVNQGARIGVS
                     GAEVIQAVKGVEVFDSSNRPLVWRVYGGRTRFLKADVQGYTTNKTMDIRQAIKTALKT
                     LPTAPSLNLNSILAEHEQLQKRIASAKNCREEGEWLKNNRTELYQQDIFNVSDQQFLA
                     LTNKGK"
     gene            4582..5301
                     /gene="mdcE"
                     /locus_tag="ACF2SP_002438"
     CDS             4582..5301
                     /gene="mdcE"
                     /locus_tag="ACF2SP_002438"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HFD2360212.1"
                     /translation="MMPNEKITLKQLIDHCFDTTKTTIKQQIVFGSGKIKNKNFNIVG
                     TVEDTSFGVDESIEMARCILDIIKNNNTDPIVLLTDVAGQKLSVRDEWLGMYAYFAHL
                     LKCLHLARQNGNKIITLIYNQAIGGSFIAFGMMADRIYALKNAKLAVMWLEGMSKVTK
                     IDIDVLRKISETSPVFAPGVENFKKLGGLHQVLDLDEVSQQLLLTLQEPNISHDNRAE
                     LGKQYGGRKEAYDIIKAIENL"
     gene            5298..5921
                     /gene="mdcG"
                     /locus_tag="ACF2SP_002439"
     CDS             5298..5921
                     /gene="mdcG"
                     /locus_tag="ACF2SP_002439"
                     /EC_number="2.7.7.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HFD2360213.1"
                     /translation="MNYLRHTLCYLDKNTITASLPAPEQLDLLQYWLKCEYPLIVTRQ
                     PEDVPCGQIQLAIPYFIPTQSQKLRASYIVDDSWVIDTKALPSLQEIFPYLELDTSTK
                     IQVYGSYCWQHLTREQYIQSSSDLDVLISYENQSLLTLSELQKHLQKTLKIDCIDGEI
                     RFPTLGDCSWTELLQIHSSDSILFKAAKKIFLVKRESLYANFPALLA"
     gene            5893..6735
                     /gene="mdcB"
                     /locus_tag="ACF2SP_002440"
     CDS             5893..6735
                     /gene="mdcB"
                     /locus_tag="ACF2SP_002440"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFD2360214.1"
                     /translation="MQTFQHSLPNPYKIARYFAKKAVQALYDEVALYPKPGLVSFIDS
                     GAHQDMNGALFFRSLFGLRHYFFQVGLHTALGSSPKKLVTFGLNAEKTMLKITGGINT
                     HRGAIFALGILCTSICKLSAKYHAFSAEDLHYAIIDQWAQYLEKEHQNSDTHGTLVTK
                     RYAVPDAKQTAIQGYKAVFHTYNELSNIIHDQTFFGLLAYQRLLLAIDDINILYRTGP
                     EGLEFARYHVQQDICQNNREKSIQKAIELHQLFSKKNISPGGVADMLGLIYFLRHVFS
                     REAK"
     gene            6732..>7676
                     /gene="mdcH"
                     /locus_tag="ACF2SP_002441"
     CDS             6732..>7676
                     /gene="mdcH"
                     /locus_tag="ACF2SP_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HFD2360215.1"
                     /translation="MKILFLFAGQGYHADSLFDIFRTDKKTINYLKTMSSAAEIDFLQ
                     NNLEMTNPYSIQSIIGAYQLTLFSRLLPLFTNHQVDLAGYSLGEISAFLGSINANPRD
                     SFKLFRYRTQLMTSIVDNNIFAEYDLLSVVGKFILEEIQPVIAKHNCYIAIINSAQHV
                     VIGGKISDLRHLITELSQYPISHAKFLGVYLPSHTDFYSDKSKQFQRFLDENYGFYSL
                     KYPVISPLELNKIYNAKQERALLAQELYTTLQWHKVCDLIGEYQYNLIIDLGPGDSMT
                     TILNKANTELFNTPLIIASHYNSLKGLCNAITSLGSSYQ"
BASE COUNT         2471 a         1479 c         1514 g         2212 t
ORIGIN      
        1 ggtaatagcg gcttataatg tcctgctttt taactgggtt cgtggagttg ggagatttaa
       61 aatgcctaag ctatcagaaa tgaattctgt tacagtgtgg tttgagcccg gtgttgggtc
      121 tggtcatatc aatgcaacta tcgctcgcat gaatgagctg tgtttattag gatttagagg
      181 ttcttttaat attatttatc taagtaacat gaagcttagt gggccaaagg aacaaatgga
      241 gctaattgcg cgcggagaat catgtaccga caaatatagt ttaagttatg gtcaagtctt
      301 atcctcgggc gagtatacac ctcctgagat aatagaaaga cttctggtgg aaaaatttcc
      361 tcatcgaatt aagcaatttt tattgaatga tgatgaaaaa ctctcaatac ctcccggtac
      421 gcatgatgag ttatgtatca gtggcgctgc cgatcaaacc agtgaggcgc cagtgaatcc
      481 aaacgaagta tcattactaa gagtacagat ggatatggct tttggttgca gtgtaatgat
      541 acaactacag cctccaagat ggttagcagg tgtatcttat gtatgtcgca gtggcagtgc
      601 aagggaaact gtctcatctc taccacataa gatagatgat gaaataaagc aaggttttag
      661 aagtataggg gcaattggcg ccagacccaa tgtagttaag attggctgct atggcatcag
      721 aaatataacc agtccaggtg taataagcac gttagtgact ttatctaaag cgaaagagcg
      781 ctgtgaaata ttagaaatag gatccgaacc ttcggttgca gattctacta aaaaggggat
      841 actagggaaa actgatttgg gtaaattcta tgctgagata gatttaatga tttgcgaagg
      901 tcaaaacacg gcaaccgaat gtttggcatt aggtgtgcca gtattgcacc ttcccaaacc
      961 tcatgcagga aatatatatc ccagtgatag agaattacga gatttaggtt ttaatatgcc
     1021 cgaacataaa aaggtatatg attccgttca cagcatgatt gctcttgctc aagattttac
     1081 aacagggtct ggtaatttat gtagctcatt acaaaaaatt agagaacatg ctgatagagg
     1141 tgctttggct caattggctg ataaaattaa tgctcggccc tctgtcttag aagacatatt
     1201 aacccgcttg gggattcagc ttgcaattaa agcggcagct cctgaagttg tacaacaagt
     1261 ggatatgaat ccaacaaagc aagtgaaaga ggctcttggt gcagcaaaaa agttagattc
     1321 aaactttcaa gatacgggtt ttaagacact attaccgtaa gcggtcaaac tattctgtca
     1381 attcgttctg ctgcaaaatt ttgtttctta tgtgcatctg tagaaattgg ttatgcataa
     1441 atttcaactc tttaatgatc aagaaactga cgcagaaaaa tggttttaat taatcaaatc
     1501 aagctcatag tgtcgagttt tagacgcttc tactattaaa aacagacatg gacatctgct
     1561 acattaagca atgcagcaag aatcataaac ttatctttaa acagtttcaa ctaaaaataa
     1621 gagataaaat ttaatattta agggggataa aatcaaatac ttgcgtcaat atctactata
     1681 ctattaacat ttcatatcac tatagttctt atccgattac taaaaaatta tggactggaa
     1741 taaaaacctc aaggattatc agagcagact cattagcatc aaaccttttt ttaaaaaagg
     1801 taaaatcgtt tctgagaaaa atatagtcgc tcttcttgaa acagcaataa aacctcatga
     1861 caaagtctgc ctggaaggag acaatcagaa acaggccgat tttctggcac gcagcctatg
     1921 ttctgtaaat ccccaaaaaa tatacggatt acatatgttg caatcggctg ttaccttatc
     1981 ggaacatctc gacttgttcg aacgaggaat tgctgaaaaa attgattttg cgtttgctgg
     2041 cccacaatca actcgtttag caattatgat ccagcgtaat caggttaaga taggcaatat
     2101 acatacttac aatgagcttt atgcacgtta tgtgatggat cttactccca atgtcgcctt
     2161 aatcactgca gaaaaagcag attattacgg taatctatac accggcgcca atacggaaga
     2221 aaccccgagt atcgttgaag caactgcttt taaaaatggc ctggtcattg tacaagctaa
     2281 tgaaatagtc aagaaactcc caagaattga cattccagga gagtgggtcg atgccatcgt
     2341 tcaagcccca tccccttcct acattgagcc tttgtttaca agagatccag ctctcattga
     2401 cgaagtaaaa attctcatgg ccatgatggt gattaaaggc atttatgctc cttatcaagt
     2461 caatcgatta aatcatgggg ttggttttaa cacttgtgca attgaactta ttctgccaac
     2521 ttatgcccag tctctgggtt taaaaggtaa aatttgcaaa cattggatgg tcaatcctct
     2581 acctacttta atccctgcaa ttgaagcggg attcatcgag tctatctttt gtgtaggggg
     2641 tgaggtaggg atgaatgagt ttattcgctc caaaccagac atctttttta ctggtcgaga
     2701 tggttcatta cgttcaaatc gcctgtatgg ccaacttgcc gggcattatg gatgtgatgt
     2761 atttattggc gcgactcttc aaatggatcc gcaagccaac agttcaacgg caaccaaagg
     2821 aagaatcgcc ggctttggtg gagcacccaa tatgggctgt gatactttag ggcgacgtca
     2881 ttcctcttac gcatggctta aagctggaca agaaaaaaat tctcataatc ctgtagcaat
     2941 gcctcgagga cgtaaacttg tgatacagat ggttgaaaca tttcaggggg caggcaaacc
     3001 aacctttgta gaaaaactgg atgcatggga actacaaaaa gaaatgcagg ctgacctgcc
     3061 gcccattatg atttatggtg atgacatctc tcatgtcgtc accgaagaag ggatagccaa
     3121 tctgttatta tgtaaaaatt cagaggagcg tgaacaagct atccgcggaa tcgccggtta
     3181 tactccggta ggtttgaaac gtgataaagc aatagtcgat gaactgcgcc atcgtggtat
     3241 cattcaacga ccagaagatc tcaacatttc attaaaagaa gctgatcgag atctactcgc
     3301 tgccaaaaac attcatgatt tagtggaaat ttcgaatgga ctttattgtc ctcctaacaa
     3361 attcaggaat tggtagacct atttatggat acgtatcaat acacattcag tttacccaaa
     3421 aaacttcaaa tatctccaat gattaaagtt ggagtggccg gttcaggtaa tttggaagtt
     3481 attattaaac caaactctaa ctgtgataaa accgatatta ttgtaaatac tgtaatctct
     3541 ggtttccgaa atacctggga tgctgtaatc gtacgctttg ttgaagatta tccttatggt
     3601 gggctatcca ttactcttaa cgacgcgggt gccactcctc ccgtcgtttc cttacgcctg
     3661 cgtcaagcaa tagaaaccta ccaaacagga tatcctaaaa aggatagcta tacggaagcg
     3721 aacgctcgca ctagaattta ttcattagtt gatgaagcca gttttacaga gtttttactt
     3781 gataaagaga caccaagtcc aaccttgcct cagttaaaca tgcaagtgga aactgatgat
     3841 ggggtcatca ttggcatcgc caaaatgaat ggaattgata tagctattgt atcgcaacaa
     3901 aaagatttca ttggcgggtc agtaggagag atacatggtg ccaaaatcaa tggtctaatc
     3961 aagtatgcaa ttaaaaatca actgcctgca atcatttttt tgattgacag tggtggagtc
     4021 cgtttgcaag aagcgaatgt aggtgagatt gaaatttctg aaatcattcg ctcaatcctg
     4081 gatgctcgct cagccggcat taaaaccata ggcgttattt gtggcaataa tggggccttt
     4141 ggtggcatgg ggatcatcag tggcaccctt gattatctta tcgttaatca aggagcacgt
     4201 attggggtat caggagcgga agtaattcag gcagtaaaag gagttgaagt atttgatagc
     4261 agtaatcgtc cattagtctg gcgagtctat ggtggtagaa cccgctttct taaagccgat
     4321 gttcaaggat acaccaccaa taaaaccatg gatattcgtc aggcgataaa aaccgccctt
     4381 aaaacgctcc ccactgcccc atcccttaat ctgaattcta ttctggcaga gcatgaacaa
     4441 ctgcaaaaac gcattgcctc agccaaaaat tgccgtgagg aaggtgagtg gttaaaaaac
     4501 aaccgaactg aattatatca gcaagacata ttcaatgttt ctgaccagca attcctggca
     4561 ttgactaata aagggaaata aatgatgccc aatgaaaaaa ttactttaaa acaattaatt
     4621 gatcattgct ttgacacaac aaaaacaaca attaaacagc aaattgtttt tggatccggg
     4681 aaaataaaaa acaaaaactt caacattgtt ggcaccgttg aagacaccag tttcggagtt
     4741 gacgagtcga tagaaatggc cagatgtatt ctggatataa taaaaaataa caatacagat
     4801 cctattgtcc tgttgactga tgtagccgga caaaaattat cagttcgtga tgaatggcta
     4861 ggaatgtatg catattttgc tcacttatta aagtgtttgc atttggcacg ccaaaatggc
     4921 aataaaatca ttaccctcat atacaatcaa gccattggtg gcagttttat cgcattcggc
     4981 atgatggctg acagaatata tgctttgaaa aatgctaaat tggcagtcat gtggctagag
     5041 ggtatgtcta aagtgaccaa gatagatatc gatgtgttac gaaaaatcag tgagacatcc
     5101 ccggtatttg ctccaggcgt agaaaatttc aagaaattag gagggttaca ccaggttctt
     5161 gatttggatg aagtctctca gcaattgcta ttgacactcc aggaacccaa tattagccat
     5221 gataatcgtg ccgaattagg caaacaatat ggcgggcgca aagaagccta tgacattata
     5281 aaagccatag aaaatctatg aactatctca gacatactct atgctatctt gataaaaaca
     5341 cgataactgc aagcttaccc gctcctgaac aacttgattt actccaatat tggttaaaat
     5401 gtgaataccc ccttatagta accaggcagc ctgaagatgt cccctgtgga caaatccaac
     5461 ttgccattcc ctactttatc ccaacgcaaa gccaaaaatt acgcgccagc tatatagtgg
     5521 atgactcttg ggtaattgac acaaaagcac tcccttctct gcaagagata ttcccttatt
     5581 tagaacttga tactagtacc aaaatccagg tttatgggtc ctactgctgg caacacctaa
     5641 ccagggagca atatattcaa tcttcttcag acctggatgt gctgatatct tatgaaaatc
     5701 aatcactcct caccttgtca gaattacaaa aacatttaca aaaaacgctt aaaattgatt
     5761 gtatcgatgg tgaaatacgc tttccgacct taggagactg ttcttggacg gaattgttac
     5821 aaatccactc cagtgattcc attttattta aagcagcaaa aaaaatattt ttagtgaaaa
     5881 gagagagcct atatgcaaac tttccagcac tccttgccta atccatacaa aattgccaga
     5941 tattttgcaa aaaaggcagt tcaagccctg tatgatgaag tggccctata tccaaaacct
     6001 ggattagtga gtttcattga tagtggggcg catcaggata tgaatggggc tttatttttt
     6061 cgcagtctat ttggcttgcg tcattatttt ttccaggttg gcctacatac tgccttagga
     6121 tcttcgccaa aaaaactggt aacatttggt ttgaacgctg aaaaaaccat gctaaagatt
     6181 actggaggga tcaatactca tcgaggagct atatttgccc ttggtatatt atgtacttcg
     6241 atttgcaaat taagtgcaaa atatcatgcc ttttctgccg aagatctgca ttacgcgatt
     6301 attgatcaat gggctcaata cctggaaaag gagcatcaaa acagcgatac acatggtaca
     6361 ttagtaacga aaagatatgc tgtacctgat gccaagcaaa ctgccattca agggtataag
     6421 gctgtattcc atacttataa tgagttgtcg aacattattc acgatcaaac attctttgga
     6481 ttattagcat accaacgttt attattggct attgacgaca tcaatatcct ttatcgcaca
     6541 ggtcccgaag gattagaatt tgcccgttat catgttcaac aagacatatg tcaaaacaat
     6601 cgggaaaaat ccatacaaaa agccattgaa ctgcatcaat tattttccaa aaaaaacata
     6661 agtcccgggg gcgtagcaga tatgttaggc ttaatttatt ttttacggca tgttttctca
     6721 agagaagcca aatgaaaata ttatttcttt ttgccgggca aggttatcat gctgacagtt
     6781 tgtttgatat ttttcgaact gataaaaaga caatcaacta tctaaagaca atgtcttctg
     6841 cagctgaaat tgatttttta caaaataacc tggaaatgac taatccatat tctattcaat
     6901 ctattattgg cgcctatcag ttaacccttt tttcaagact gctgcccctt ttcacaaacc
     6961 atcaagttga cctggctggt tacagtttag gagaaatcag cgctttttta gggagcatta
     7021 atgcaaatcc tcgagattcc tttaaattat tcaggtatcg tacccaactc atgacatcga
     7081 tagttgataa caatatcttt gcagaatacg atttgttgtc tgtcgttgga aaatttatat
     7141 tggaagaaat tcaacctgtt attgcaaaac ataactgcta tattgctatt atcaattcag
     7201 cacaacatgt ggtgatcggc ggaaaaattt cagatctcag gcacttgatc acagagcttt
     7261 cccaataccc tataagtcac gcgaaatttt taggagtgta tctgccttct catacggatt
     7321 tttactctga taaaagcaaa caatttcagc gatttctaga tgaaaattat ggtttttatt
     7381 ctctaaaata ccctgtcata agcccgctag aactaaataa aatatataat gccaagcaag
     7441 aaagggcttt gcttgcccaa gaactatata caacgcttca atggcataaa gtttgcgatt
     7501 taatcggcga atatcagtat aatttgatta tagatctcgg ccctggcgat tccatgacta
     7561 ctatcttgaa caaagcaaac acagaattgt tcaatacacc cctaattatt gcatcacatt
     7621 acaatagctt aaaaggactt tgtaacgcca taacatcgct tggctcatct tatcaa
//
LOCUS       DAXLDT010000139         7520 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXLDT010000139 DAXLDT010000000
VERSION     DAXLDT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.139"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(115..705)
                     /locus_tag="ACF2SP_002442"
     CDS             complement(115..705)
                     /locus_tag="ACF2SP_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1124 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360216.1"
                     /translation="MRMKMFSKTIPLTSQEAFEILCTTDYLKKISKIIFNFQQLFNVE
                     RSTLLSHYKLNPKISNNREFLQDLEARYDRLNHAVQNNEPYPFLYGDVCLLKEYLQVI
                     LGYYLEQLKEEQPVAKKNLRRIKGSHKFSTLMSDISKGEHPKLGKKDSEILIKYTINF
                     CAESTMWDDVKTISDLVIKPFLFDHKDEEGFSYCNP"
     gene            987..1682
                     /locus_tag="ACF2SP_002443"
     CDS             987..1682
                     /locus_tag="ACF2SP_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD2360217.1"
                     /translation="MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLV
                     QIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELK
                     PNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKI
                     GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLRIAVAKDNFKLRDKVNLILADLMQNG
                     TLSRLQHKWISTT"
     gene            1866..5297
                     /locus_tag="ACF2SP_002444"
     CDS             1866..5297
                     /locus_tag="ACF2SP_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD2360218.1"
                     /translation="MKILKTTEKKSRKPTPKENRALPLQLVQNTEPDRTYYLRSTVEG
                     IGSFLSTRVRELYNTLAMTFSLDSMDWSSFKKYIWPTISSGAALLLGDYLTRSMSEYQ
                     TGSPEYDFIFKFLFGTAILGYCALNRITDRKFTSQVELSAAVASEVMAITYMYSSVFK
                     GTNYLLNDSISDEYSLIAGLGTSALLVPSGISYLTQKIQEFLVRIQYNKGAQRSDTAV
                     ISSGLTPISNLYFQVNHFIASELMISSRAFQLGLISHNIRYADRIMQKFRNLPALLAD
                     LAPSTIKKMRIQQQKLEHDYEYNHKLHQVLRFTADGPMFISIPRYQLRTGDLVYCDSS
                     IDLACVPVSGELLALRRDLKGEFTQTLEQKKFSVNLKAQNGEDVWIEHETKPDFTSHY
                     KKVDLHAVRDGKQAGVLVGDKLNLYGNDNIFIQIKPEKELLLSSNYEKKAVINEIIAE
                     RKQRSVLYSILGSVIMAAFLQRDITAMPAETLRLMFTLFQTMIPFSEAFLRETVNSRL
                     MKKLNSDLGDQPFGTIDALRVVDLCNALGGYYRDRFPNGVAIISDKTGTLTTTRMDVL
                     GLWTNDMAPEVQKTLKEKEGLLLPEEIQWLQTFEVFCNAFTNNKKELEPEEHAILELF
                     QSLLDNRQCLEVITLGNNHFKKEMISKDTKKEIETFHLGLYRTFGGRFTLVQDGSDHY
                     LVFCGVPKQEAFKETPLLKAYTTMQSRTGVLSRDWCLARGKLSEREFTILKELFSKDD
                     KKQIEHFILQNKLLLENLMYYGTFIIDNPVKKGAEKFIAQCREVSVPVFVATGDTTKA
                     AENIAKVLCPANAGKIVTLRADQIDEKGVEFLNEENYPSDSTVIFSGINEAILNRFQK
                     LMDREGAKRPVIIFAEMSTEGKGILARYLKDHHYFVVANGDGTNDVMMMKNSNVVIAH
                     HSDDGTFAPGVDGLTNISEEQLRRLFGSQKTFYELFDINLPKSLFVQKFAPLANSQEK
                     PTMALALKSGKMTFDLAKTVGADVTEMNQQHWYSVVFDLLWLWIAFYEINESSDLPMD
                     NRNINASRLISNTMGIALIIAVFQSLANYALFNESTNLTSMLIMLSFLPLILKSIFSG
                     FRMVQDSLYPQVEVTEVEEEGDTSTNRSVLSYFGTFFSRKPAKQLEGLNCAPVLK"
     gene            complement(5392..6900)
                     /locus_tag="ACF2SP_002445"
     CDS             complement(5392..6900)
                     /locus_tag="ACF2SP_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HFD2360219.1"
                     /translation="MAKKRIERLIFSEVECNMKKYSMVIADKPIPYEKLFDTVHCIAT
                     HFQNKALSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLRDAQPI
                     DLIIEASKLFLLEKINWSATGIKKIFALGENQNHPYFSFNELLNEPANYPKHSIDYKA
                     PAVIFYTSGSTGEPKGVVHTLSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIE
                     TFSTLSREGTTLVHDGFDMDWYLATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSF
                     RGIYTGGDALPVAIQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVEG
                     VTLRLINATGEDVSCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGTQDE
                     QGYFYYKGRIKNIIIRNTSNIMPGEVEAAIYKHPAINAAAVIGVPDEEEGEVPIAFVV
                     VKKSNQLTKEELYSFLFEQIAQYKIPAKIYFIDEMPLTNSGKINHKKLYDYL"
     gene            6983..7447
                     /locus_tag="ACF2SP_002446"
     CDS             6983..7447
                     /locus_tag="ACF2SP_002446"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFD2360220.1"
                     /translation="MDFIKRTIELARENVGHGGRPFACLIVQNGKILAEATNTVAQTH
                     DPTAHAEVNAIRIATAKLGCEHLYDCDFYILAHPCPMCLAAMYYCSPRKVIFITTRDE
                     YKTYYVDDRKYFTLENFYQEINKPWRERKMPMEHRPDKEAIEVYKMHQKKKS"
BASE COUNT         2332 a         1275 c         1467 g         2446 t
ORIGIN      
        1 tgttagctgc gcgatcaaga ggctcttcaa ggtagggctt ggaggggatt gggcagtatt
       61 cgatttagag atttcgtcaa atccaggtca ctgccctctc atcaattctc cagcttatgg
      121 attgcaataa gaaaaacctt cttcgtcttt gtgatcaaat aaaaatggtt ttattacaag
      181 gtcactaatt gtttttacat catcccacat agtgctttct gcacagaaat taatggtgta
      241 cttgattaaa atttctgaat cctttttacc aagcttagga tgctcccctt ttgagatatc
      301 agacattaga gttgaaaatt tatggcttcc tttgatcctt cttaaatttt ttttcgctac
      361 cggttgctct tctttaagtt gctccaggta gtagcctaaa atgacttgga ggtattcttt
      421 caataaacat acatcgccgt ataaaaaagg gtaaggttca ttattttgta ctgcatgatt
      481 gaggcgatca taacgagctt ccagatcttg aaggaattct cgattgttac ttattttggg
      541 gttgagcttg taatgagaaa gaagagtact tctttcaaca ttaaacaatt gttgaaagtt
      601 aaagataatc tttgaaattt ttttaagata atctgtagta cataatattt caaaggcttc
      661 ctggctggtt aatggaattg ttttagaaaa cattttcata cgcatgacgt gatctctggt
      721 tgatttagat ttaatttaaa tctaaatgat taaaattcga cattatataa taatgcatta
      781 gatagataaa taaacatagt gattttttta tattcttttt tggtttttat aattttagct
      841 gttaattgca taaccaatcc tggatatatt actcgtgtaa tcatgggtaa attattcttt
      901 taaatcaatg gcataaaaaa tatttggctg atttttttct gattaatcta tacttagtga
      961 atcgattcca aacaatagtt atatctatga ttctaaagcc gggaaaatta ttggtggcta
     1021 ctgcttatcc agatccaccg tttgatattg agccaggtga tggttttgat ggtgatttaa
     1081 taaaagaaat ttgtcgaatt ttaaatttgc agctagtcca gatcaaatat gagggagaaa
     1141 attttaatga tattttttta ggcttggcta ataaagaata cgatgttgtc atttcaggaa
     1201 cgactattac accaaaccga tcaaaacaag tacttttttc gaatccctat ttagagttta
     1261 atcagggtat cgcggttaat actgaattaa agccaaatgt ttcttctttt gaagatttga
     1321 aaggtttggt ggctggtata cagaaaggca acacctcaga tgaagttgct caggatttat
     1381 tacgtaagaa catactcagt gatattaaat attatgcata caatgaaata gaattagcca
     1441 tacaggatct gtctgccggt aaaattggtt tggtcattaa attatatcct gttatttcct
     1501 gtttaattcg aaactataca aacctggctg ttcctttgca ggctgaaaca catgaaaaac
     1561 ttcgtatcgc ggttgctaaa gataatttta aactacggga taaagtcaat cttattttgg
     1621 ccgatcttat gcaaaatggt actttaagtc gtctgcaaca taagtggata agtacaacct
     1681 agggtaaatt aggccaatgt cagtttcaac tcaagaaata accttattta aggaatctta
     1741 tttttcttga atcgttctgt aattattttt caaatcctct tgaactttaa tacagataag
     1801 ttaattatca ggttgagctc taaaaatcca atagagtgtt attggagtac aaaaggattt
     1861 ataacatgaa gatattaaag acgaccgaaa aaaaatcgcg aaaacctact cctaaagaga
     1921 acagagcttt accgcttcaa ttggtacaaa atactgagcc ggatcgaacg tattatttga
     1981 gaagtactgt agaaggcata gggagttttc tttctacccg tgtaagggaa ctttataaca
     2041 ctttagccat gactttttct ttagactcta tggattggag cagtttcaaa aaatacattt
     2101 ggccaacgat atcctctgga gcagcattgc tgcttggtga ttatctaacc cgatccatga
     2161 gcgaatatca aacgggaagc ccggaatatg attttatttt taaatttctt tttggtacag
     2221 cgatattagg gtactgtgct ttaaatcgga taactgacag gaaatttact tcccaggttg
     2281 aattatctgc tgcagtagcc agtgaagtca tggcaataac ctacatgtat agttcggttt
     2341 tcaaaggcac caattattta ttgaatgata gtatttctga tgaatattca ttaattgccg
     2401 ggctcggaac cagcgcgcta ctggttccga gcggaatttc ttatctaaca caaaaaatac
     2461 aagagttttt agtgcgaatc caatacaata agggtgcgca acgctcggat acagcagtga
     2521 tttcaagcgg gctcacacca atatccaatc tttattttca agtcaatcat tttattgcca
     2581 gcgaactgat gatcagttca cgcgcatttc agctggggct catttcacac aatattcgct
     2641 atgcggatag aataatgcag aaatttcgta acttgcctgc attattagcg gatttggcac
     2701 catcaacgat aaaaaaaatg cgtatccagc aacaaaagct tgagcatgat tacgaataca
     2761 atcataagct acatcaggtt ttaagattta cagcagatgg cccaatgttt ataagtatcc
     2821 caaggtatca attacgcaca ggagatctgg tttattgtga tagcagcatc gatttggctt
     2881 gtgtaccggt ttctggggag ctgcttgctt taaggcgtga tttaaaagga gagtttactc
     2941 aaactctgga acaaaaaaaa tttagcgtta acctgaaagc ccaaaatggg gaagatgttt
     3001 ggatagaaca tgaaaccaag cctgatttta cctcgcatta taaaaaggtg gatttgcacg
     3061 ctgttagaga tggcaaacag gcaggagtat tggtaggcga taaattaaat ttatatggta
     3121 atgataatat ttttattcaa atcaaacccg agaaagagct tttattaagc agtaactatg
     3181 aaaaaaaagc agtcattaat gagattattg ccgaacgaaa acaaagaagt gttttatatt
     3241 ctattttagg ctccgttatc atggctgctt ttttacaaag ggatattact gcaatgcctg
     3301 cagaaactct aaggctcatg ttcacactct ttcagactat gattcctttt tctgaagctt
     3361 ttttacgaga aactgtgaat agccgtttga tgaaaaaatt gaatagcgat cttggggatc
     3421 agccttttgg gaccatagat gctcttcgtg tggttgattt atgtaatgct cttggtggtt
     3481 attatcgtga cagatttccc aatggtgttg ccattatttc agataaaacc ggaactctta
     3541 caactacaag aatggatgta ttagggttat ggactaatga tatggctcct gaggttcaga
     3601 aaacattaaa ggaaaaagaa ggcttgctcc tgccagaaga aatacaatgg ctgcaaactt
     3661 ttgaagtgtt ttgcaatgcg tttactaaca ataaaaaaga attggaaccc gaggaacatg
     3721 ctattttaga gttatttcaa tccttactag ataatcggca gtgtctggaa gtgattacct
     3781 tggggaataa tcattttaaa aaagaaatga tttccaaaga caccaagaaa gaaattgaaa
     3841 cgtttcattt gggtttatat cgtacgtttg gtgggcgctt tactttggtt caggatggtt
     3901 ctgatcatta tctggtcttt tgtggagtgc ccaaacaaga ggcttttaag gagactcctt
     3961 tgctaaaagc ctatacgact atgcaaagtc gtactggagt gttatctcgt gattggtgtt
     4021 tggctcgagg taaactaagt gaacgcgagt ttactatttt gaaagaatta tttagcaagg
     4081 atgataaaaa gcagattgag cattttattt tgcaaaataa attattgctc gaaaatttga
     4141 tgtattacgg tacttttatt attgataatc ctgtaaaaaa aggggcagaa aaatttattg
     4201 cccaatgtcg ggaagtatca gttccagttt ttgtagcaac aggagacacg accaaggcag
     4261 cagaaaatat tgctaaagta ttgtgcccgg ctaatgcagg aaaaattgtt acgctgcgtg
     4321 ccgatcagat tgatgaaaaa ggtgttgaat ttttaaatga agaaaattat ccctctgact
     4381 ctacagttat attttcaggt attaatgaag cgatattgaa cagatttcaa aaactgatgg
     4441 atagagaagg ggcaaaacga ccagtcatta tttttgctga aatgagtact gagggaaaag
     4501 gtattttagc tcgttatctt aaggaccatc actattttgt tgttgccaac ggggatggta
     4561 ccaatgatgt tatgatgatg aaaaattcca atgtggtgat agcgcatcat tcagatgatg
     4621 gaacttttgc tccaggagtg gatggcttaa ccaatattag tgaggagcag ctaagacggt
     4681 tgtttggttc ccagaaaact ttttatgaac tgtttgacat taatctaccc aaaagcctgt
     4741 tcgttcaaaa atttgctcct ctagccaact cacaggaaaa gccaaccatg gccttggcac
     4801 tcaaaagtgg aaaaatgacg tttgatttgg ctaaaacagt tggagcagat gtcacagaaa
     4861 tgaatcagca gcattggtat agcgttgttt ttgacttatt atggttgtgg attgcttttt
     4921 atgaaataaa tgaaagctcc gatttgccaa tggacaatcg gaacatcaat gcttcaagat
     4981 tgatttccaa taccatgggg attgccttaa taatcgctgt ttttcaatcc cttgccaatt
     5041 atgctctgtt taatgaatct acgaacttaa ccagtatgct tattatgttg agctttttgc
     5101 ctcttatact aaagagtatt ttctcaggtt ttagaatggt tcaggacagc ttgtacccgc
     5161 aggtggaagt aaccgaagtt gaggaggaag gagatacaag tactaacaga tctgtcctaa
     5221 gctattttgg aacttttttt tcacgcaaac cagcaaagca attggagggt ttaaactgtg
     5281 cccctgtatt aaagtaactt ctttgcataa ccaggatttt ttattttgcc actatgtgta
     5341 aatgtttctg tgatgctcat tgactgcatg caagctaggc attttttaat tttaaagata
     5401 atcatacaat ttcttatgat taatttttcc gctattagtc aagggcatct catcaataaa
     5461 ataaattttt gcaggtattt tatattgagc aatttgctca aataaaaaag aatataattc
     5521 ttcttttgtc aattggttac tcttttttac gactacaaaa gcaatgggta cttctccttc
     5581 ttcctcgtca ggtacaccaa tcacagccgc tgcgttaatt gctggatgtt tataaattgc
     5641 tgcttccact tcaccaggca taatattgga tgtattgcgg ataataatgt ttttaatccg
     5701 acctttataa taaaaataac cttgttcatc ctgagttccg caatcccctg ttttaaacca
     5761 accttttata aatgctttgc gggtttcttc aggattattc caataactat gcatcaccat
     5821 gtcaccttta accagaattt ctcctgtttc accacaactc acgtcctctc cagttgcatt
     5881 gataaggcgt aatgttactc cttcaaccgg tttgccaata caggattttt gagtcaaatc
     5941 aggggtcctg cagactgtta gccagatggc ttccgtcatt ccataaccca cttgaattgg
     6001 tttaccagcg caagccagaa atttatcctg gattgccacg ggcagtgcat ccccacctgt
     6061 ataaattcca cgaaatgatt ggaatgtttt tgtattacag cgtccagagt ctaataactt
     6121 tatataggta tcaatatgat gtacggctag cgttggtttt tcatgtcgca ctgtcgcaag
     6181 gtaccaatcc atatcaaaac catcatgaac taacgtggta ccttctcgtg ataatgtaga
     6241 gaatgtttcg ataaatccac ttacatggca ttgtggctca cagattatca ttttgtcgtc
     6301 agcggtaatg ttttccagtg ccaatgaagt cgagtcaagc attgcttcaa tggaagaaag
     6361 cgtatgaaca actcctttgg gttctcccgt tgaaccggag gtataaaaaa ttactgcagg
     6421 cgctttataa tctatgctat gttttgggta attcgctggt tcatttaata attcattaaa
     6481 agaaaaatag ggatgattct gattttcccc cagggcaaaa atttttttaa ttcctgtcgc
     6541 tgaccaattg attttctcta gcaaaaataa tttacttgct tcgataatta aatcaattgg
     6601 ttgggcatcg cgcaatacct tttcaagctc aggtggcgca tagcggcgat taacaggcat
     6661 ggcaatacat cctgattgaa aacaggccag gtaaatggct ataatttcaa ggctatttgg
     6721 caacataaaa gcgatacggc ttccagagag tgctttattt tgaaagtgag tcgcaataca
     6781 atgcactgta tcaaatagtt tctcgtaggg tatcggcttg tctgcaataa ccatagagta
     6841 ttttttcata ttgcattcaa cttctgaaaa aataagcctc tctattcttt ttttagccat
     6901 tatgctttcc tggatagttt catttgttac tttttatatt atgggaaata attaataatt
     6961 atgggctaaa aatgggaaaa aaatggattt tataaagcgt actatcgagc tggctcgaga
     7021 gaatgtggga catgggggca gaccatttgc ttgtcttata gttcagaatg gaaaaattct
     7081 tgctgaagct accaatacgg tcgcgcaaac ccatgatcct acagcccatg ctgaagttaa
     7141 tgcgatacgg attgctacag cgaaattagg ttgtgagcat ttatacgatt gcgattttta
     7201 tatacttgct catccttgcc cgatgtgcct ggccgcaatg tattattgta gtcccaggaa
     7261 agtgatcttt atcacgacga gagatgaata taaaacctat tatgtagatg atcgaaaata
     7321 cttcactctt gagaattttt atcaggagat caataagcca tggagagaac gtaagatgcc
     7381 tatggagcat agaccggaca aagaggcaat agaagtatat aaaatgcacc agaaaaaaaa
     7441 gtcttagtcc aaataactgt ttaatattta aaacagtttg attttcaacc tttaaaaaac
     7501 ttttatgttt taaagttcat
//
LOCUS       DAXLDT010000140         7508 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXLDT010000140 DAXLDT010000000
VERSION     DAXLDT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.140"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            398..1912
                     /locus_tag="ACF2SP_002447"
     CDS             398..1912
                     /locus_tag="ACF2SP_002447"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFD2360221.1"
                     /translation="MSYMKIEELLLKDTMRRAQIPGVSIAYVNNQGVISTQEIGLTDG
                     FGLVKMSNDPTRCPFQELVLGSKSELGVGTKNTVITFNKELYYVDQAEKIVRKIPLTE
                     ANKIVYEMLHAKCTETYQLADSNEHELIAALTGHMPPTEVKPETVFGAASLSKPVFAY
                     LVQKLIQTNAKNQSEAGFDQFILPEKLKHFDLDTPLFHIIPLEEFNIDGMKFDLSDES
                     IVAYSKVLTARMVLSHTTGLTHGEMKFQFLPNSEEKEHGYSNVGIIYLQQVIEKLTGS
                     DLETLAQKHVFEPCGMIHSTYGLKACAANSLRTTACDYANFVKHLIHDSTIENPFVPH
                     AYMTKDKGQAGAIRIAKGNIPDTILQHVAWGLGWGLQTNDEGRVITAYHSGDMNDYRA
                     WVVIDLQDKDKKNAVVFFANSHNGHILAEQIIPKTMQMEQAANYFFSKWGFARNLAEL
                     GGKTTRLGIKSSSGSSDSSPDTSIQDEPVEIKKETEKSDTSSIETYTSGWTFNA"
     gene            complement(1889..2809)
                     /gene="lelA"
                     /locus_tag="ACF2SP_002448"
     CDS             complement(1889..2809)
                     /gene="lelA"
                     /locus_tag="ACF2SP_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator LelA"
                     /protein_id="HFD2360222.1"
                     /translation="MAIPFKRINLNLILHLDALLSEKSVSAAAEKVFVSQPAMSSSLK
                     QLRELFKDPLLIPGKGEYFLSPKAKELQPQLEQVMFFIRNILQPNERFDPLRTKRSFK
                     IALPDYVELILLPSLMAALEKLPHIVIETTIQSMADDPHLFIDENVDLAIGAINENSL
                     SAQLKVEKLYEEEIICFASKNNPLINRPLSLEDYISNKHIAFSFNQERYSHSAEYLYK
                     RKLVRNDMLFVKNILPAIFAVANSDAVISTAPSFLINMFARQFNLGMQALPFNLEPTP
                     IYLVTHSKSQYDEGVSWLAKLISSSIKSPS"
     gene            complement(2993..4507)
                     /locus_tag="ACF2SP_002449"
     CDS             complement(2993..4507)
                     /locus_tag="ACF2SP_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFD2360223.1"
                     /translation="MFGELFASSIVLGVRYYSNQAYQAVAGLLGQIERFGIGYTREFI
                     RDPKSMIQSLTSKARSSEAGYSVVSLGYLGGNYAVITPKSQSQLTELLNQFDQEKSGR
                     RGHASFKFFSGNDFFIISDTGHAALSSRKNLSRFLVPLRLVNPTITEVEKRVDCDADI
                     QLRHTICGIVRAVMVENMLGIKQLPDNTYDVMSSYRNDVKRWGAFPFPELLNFMPSLR
                     QKRDVYRAFSKGILEQEFEKLVEVLHDDPNPGNTNLIAASVVSLFKDENPTLSNEELL
                     SALRSLSIEDIRKYFENPIVQSLPMILKAADNLTDAIVLCLEQIASDPDKFAMLRDEM
                     DRSHLAIEEGIDVRALKTLPVLDAFYKESVRFDAPVAVPRYTQNGYSSDSMTIPPNTM
                     MIFDLHALAKGEQYWTNPEEFDPTRFLQQEKEQVTEEGGMNSNNKNHTVGQFPFIPFS
                     VGLRNCPAFAVTESLFKVTIAKFVSDYQLQLVKKDEEDSTLHITSRDKSFGLMQ"
     gene            4805..5719
                     /locus_tag="ACF2SP_002450"
     CDS             4805..5719
                     /locus_tag="ACF2SP_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360224.1"
                     /translation="MATTLIQTPSYTKNLTLNLDDYPGGVAIWGALPALFDTSNQGFD
                     RGVHVHARLADSSKKVIDATYDHVTIISGYRIFTITEEAAVHFSMSAIFDIKITSLTC
                     QHCSQLITSVGYAAVRPSRQHQCNHCSEITTTTSECISNPIMLLKELIGDEQVKRPAV
                     IPNRTIAIDPDKYSGGIQIWGSNPSIIWTAKRLEESAIHIHAYNENGKRIIDNTYGSV
                     SLDGHKLDIEMIRVLQIQLALPNLALLLTTVYCPHCGVEQFDRGIWAVSAHNHRVCLL
                     CKQTFISQDVISNPAFDVLTHVSGVISQ"
     gene            5716..6117
                     /locus_tag="ACF2SP_002451"
     CDS             5716..6117
                     /locus_tag="ACF2SP_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFD2360225.1"
                     /translation="MSTLKTIFKIGALIFNEKNQLLAVHKKGKPPMELIVPGGVMEEN
                     ESDEETLRREIKEELDSDIISFQFYKQFEDKAIYEEKWLVMRTYIVTLGNAPKPANEI
                     DQLVWLGHDYHQSGYRFGSILGKQIIPDFFK"
     gene            complement(6201..7355)
                     /locus_tag="ACF2SP_002452"
     CDS             complement(6201..7355)
                     /locus_tag="ACF2SP_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360226.1"
                     /translation="MKVLFDKTAQNNPGSGITPSSKSNEICTSSICMKSKLPADVVAK
                     HLHGLQSTMVDRRNPDLSDSKSLMKSAKAHAILKTGTEIDMLTATGISEDDLPSSKGA
                     IPMTAPEELTMKATWKNKNQERGISGRDASVFGGLGVLVISPDTKVMNIPKMNALAVP
                     ASFLAKYGAELIPLDQPFTLDTVDKSSLENLYLVEYQWDADYIQDYVMNIRGGGGLFV
                     ETHPFPHVFTPLSPACRGALILGVERKDGAFDFAAFEIPFGYTMKIASNVIHGDSFFV
                     GPYAIALTETELADSVLLKQDTPQRDIQRVTQVPTKVFTIFLIEETKLASQINRKMMA
                     DKICQEGLSGKRLGFFKESSPKVSTEARHCSDAEQDIHDQQFGLVPCLTK"
BASE COUNT         2357 a         1495 c         1404 g         2252 t
ORIGIN      
        1 tctgaaatac ccttttaaga cttaagtggg ctaacctctc aagtttttct tcgtgtactt
       61 tcaatttcca accttatttt tcaataaagc aaattcaatt taactcagca tctataagaa
      121 aaatgtatac aaaatacaca aaaattgatt ttaatcgcaa tcagttaata tttattgttc
      181 aggagaattt cataatgaac tacaagctaa tattagccac aggcagcatc ctgacagaag
      241 agctctcttc tatccctcgc tatattgtac caatccagca aaaaccgggg tattagcggg
      301 tttttacgat atttttgcga aaaaaaattt tgagtaaatc aaacacgtca atacccaaag
      361 tataatttat ggccaaatta tttttttgag tgaatttatg tcgtacatga aaatagaaga
      421 gcttcttcta aaagatacca tgagaagagc ccagatccct ggggtatcca ttgcttatgt
      481 aaataatcaa ggagttattt ccacacaaga aataggactt acagatggat tcggccttgt
      541 taaaatgtca aatgatccca ctcggtgccc atttcaagag ttggttctcg gatctaaaag
      601 tgaattaggt gtgggaacta aaaacacagt catcacattt aacaaagagc tttattatgt
      661 cgaccaggca gaaaaaattg tccggaaaat cccgctgaca gaggcaaata aaatagtcta
      721 tgaaatgttg catgcaaaat gcaccgaaac ctatcaatta gccgatagca atgaacacga
      781 actcattgca gctctaacag gtcatatgcc accaactgaa gtgaagcctg aaaccgtttt
      841 tggcgcagcg tccctaagca aaccggtatt tgcttattta gtacaaaaat tgatacaaac
      901 caatgcaaaa aatcaatctg aagcaggttt tgatcaattt attttacctg aaaaattaaa
      961 gcattttgat ttggatactc cccttttcca tattatcccc ctggaagaat tcaatattga
     1021 cggaatgaaa tttgacctgt ctgatgaatc tatcgttgct tattccaaag tactaaccgc
     1081 caggatggtg ttatcgcata ccactggact tactcatggg gaaatgaaat ttcaattcct
     1141 gcccaattct gaagaaaagg aacatgggta ttcgaacgtt ggaattattt atttacaaca
     1201 agtaattgaa aaactgactg gctcagatct tgaaacactg gcccaaaaac atgtgtttga
     1261 accatgcggt atgattcata gcacttatgg gctcaaggca tgcgcagcca attctctacg
     1321 cactacagca tgtgattatg ccaatttcgt caagcattta atccatgaca gcacgattga
     1381 gaatcccttt gttccacacg cttacatgac aaaagacaag ggacaggcag gagcaatcag
     1441 aatagccaag ggcaatatac ccgatactat tctgcagcat gttgcatggg gcttaggctg
     1501 gggactacaa accaatgatg aaggaagagt tatcacagcc tatcattcag gcgatatgaa
     1561 tgattacaga gcgtgggtag tgatcgattt acaggataag gataagaaaa atgccgtggt
     1621 atttttcgct aactcgcata atggacatat tctcgctgaa caaattattc ctaagaccat
     1681 gcaaatggaa caggctgcca attacttttt ttcaaaatgg gggtttgcca gaaacctcgc
     1741 tgaattaggc ggaaaaacta ctcgactggg tattaaatca tcctcaggta gctctgatag
     1801 ctctcctgat acttccattc aggatgagcc ggttgagata aagaaggaga cagaaaaatc
     1861 agatacttcc tcaatcgaga cctatacttc aggatggact tttaatgctt gaactgatca
     1921 gtttggctaa ccaggagact ccctcatcgt actgactctt gctatgggtc accagataaa
     1981 taggagttgg ttccagattg aaaggtaaag cttgcattcc cagattaaac tgtctggcaa
     2041 acatatttat caaaaaactc ggggctgtgg aaatcaccgc atcggaattg gctactgcaa
     2101 aaattgcagg caagatattt tttacaaaaa gcatgtcatt acgtaccagc tttcttttat
     2161 ataaatattc agcggagtgg ctataacgct cctgattaaa actaaatgcg atatgtttat
     2221 tgcttatgta atcttccaaa gacaatggcc tgtttatgag tggattgttt ttgcttgcaa
     2281 aacaaataat ttcctcctca tacagttttt caactttgag ttgcgcggat agagaatttt
     2341 cattaatagc tcctatggcc aagtctacat tctcatcgat aaatagatgt ggatcatcgg
     2401 ccattgactg aatcgttgtt tcaataacaa tatgaggcag tttttcaagc gctgccatca
     2461 gagatggcaa cagaattaat tcaacgtaat caggtaaagc gattttaaat gatcttttgg
     2521 ttcgcaaggg atcaaatcgc tcattcggtt gcagaatatt tcttatgaaa aacattactt
     2581 gttctaattg tggttgtaac tctttggctt ttggagagag aaaatattcc cctttaccag
     2641 gaattaatag cgggtcttta aatagctctc tcagttgttt taaagaagaa ctcattgccg
     2701 gttggctgac aaacactttc tctgcagcgg cactgacgct tttctcgctt agtaaagcat
     2761 ccagatgtag tatgagattt aaattaatac gtttaaaggg aatcgccatt cgatcctctc
     2821 ggcaattgtt ctcttcttat attagtataa gaaatttaaa agtatccaat atgaataatt
     2881 cattatcaag acagggtgta ttttgagaag ggttacaata ccacttaccg aaccaggtaa
     2941 cgctgtcaaa tagacctcat tacctggcat caatctattg ttctttccag tgttattgca
     3001 ttagtccaaa agatttatct ctggaagtaa tgtgtaatgt tgaatcctcc tcatcttttt
     3061 taaccaattg aagttggtag tctgaaacaa acttggcaat cgttacttta aataaggatt
     3121 cggtcactgc aaatgccggg caattgcgca gtcctactga aaagggaata aaggggaact
     3181 gacctacggt atgattttta ttgttactgt tcataccccc ctcttcagta acttgttctt
     3241 tctcctgctg taagaaacgg gtagggtcaa actcttcagg attagtccag tattgttcac
     3301 ctttagctaa agcatgtaaa tcaaatatca tcatggtatt gggtggtatg gtcattgaat
     3361 cggatgaata gccattttga gtatatcttg gcactgcaac aggtgcatca aatcgcactg
     3421 attccttata aaaggcatct aatacaggta aagttttaag agcccttaca tctatccctt
     3481 cttctatggc caaatgcgat ctgtccattt catcacgaag cattgcaaat ttatccggat
     3541 ctgatgcgat ttgctccaga caaagaacaa ttgcgtcagt gagattgtct gctgctttta
     3601 atatcatcgg aagcgattgc actataggat tttcaaaata ttttctaatg tcttcaatgg
     3661 ataaagacct taaggcagag agcagttctt cattggaaag agtcgggttt tcatctttaa
     3721 ataaactaac cacagaagct gcaattaaat tagtgtttcc tggattagga tcatcatgaa
     3781 gtacttcaac cagtttttca aactcttgct ctagaatacc ttttgaaaaa gctcgataaa
     3841 catcccgttt ttgtctcaaa ctcggcataa aattgagtaa ctctggaaaa ggaaatgccc
     3901 cccatcgctt gacatcattt cgataagatg acatcacatc gtaggtattg tctggtaatt
     3961 gcttgatgcc cagcatattt tccaccatca ctgcgcgaac aattccacag attgtatgtc
     4021 gaagttggat atccgcatca caatcgacac gtttttctac ttctgtaata gtgggattaa
     4081 ccagccttaa gggcacaaga aaacgggaca ggttcttccg cgatgacaga gcagcatgtc
     4141 cagtatctga aataataaaa aaatcattac cagaaaaaaa tttaaacgaa gcatgccctc
     4201 ttcggccaga tttctcctgg tcaaattgat tcaataattc agtcagttga gattgagact
     4261 ttggagtaat gaccgcgtag tttcccccca aatatcccaa actgacaaca ctatatcctg
     4321 cttcactgct tcgtgctttg gaagttaatg actggatcat cgatttagga tcacgaataa
     4381 attctctggt atagccaata ccaaatcgtt ctatttgccc caaaagacct gccaccgcct
     4441 gataagcctg attggaataa taacgaacac ccaacacgat tgaacttgca aataactcac
     4501 caaacataaa tcatcccaaa taataggcca aaaaaattgg actatagtaa tacatttaga
     4561 aaatattgaa cacacccagg cgttttttta tttcatgtgg gttgattata tgatgcttat
     4621 tattcacccc tttcaaatac caggaaatga atccgacacc gatacttgtc aagaattagc
     4681 aggagagata caatgacttt gcctaaaaag ttatggtctt cagcaagtct cgattcctcc
     4741 ggtgctgggt ataaagacat aattttggtc gagggatact ttcgttttta atacgaggta
     4801 tcctatggcg acgacattaa ttcaaacacc ctcttatact aaaaatctaa ctctcaacct
     4861 tgatgactac ccgggaggag ttgcaatttg gggtgcatta cccgctcttt ttgatacttc
     4921 aaaccaggga tttgatcgag gcgtccatgt tcacgcgcgt cttgccgatt catctaaaaa
     4981 agtgatagac gcgacctatg atcatgtcac aattatttcc ggatatcgta tttttacaat
     5041 tacagaggaa gctgctgttc atttcagcat gtccgccatt tttgatatca aaattacatc
     5101 attaacatgt cagcactgct ctcagttgat tacttccgtt ggttacgctg cagtcaggcc
     5161 atcacgccag catcaatgta atcattgtag tgaaatcacg actacaactt cagagtgcat
     5221 cagcaatcca attatgttac ttaaagaatt gataggagat gagcaagtta aaaggcctgc
     5281 cgttatcccc aatcgtacca ttgcaattga tcctgataaa tacagtggtg gtattcaaat
     5341 ttggggctct aatccatcaa ttatttggac tgctaaaaga ctggaagaaa gcgctatcca
     5401 tattcatgct tacaatgaaa atggcaaaag aattatagat aatacttatg gaagtgtttc
     5461 tttagatggt cacaaactgg atattgaaat gattagagtc ctacaaatcc aattggcatt
     5521 gccaaatttg gcattactcc tgacaaccgt gtattgtcct cattgtggtg tggaacagtt
     5581 tgatcggggg atttgggctg tttctgcgca taatcacagg gtttgcttac tatgtaaaca
     5641 aacttttata tcccaagacg tgatcagtaa ccctgctttt gacgtactaa cccatgtttc
     5701 tggagtgatc tctcaatgag cacattaaag actatattta aaataggcgc gcttattttt
     5761 aatgaaaaaa atcagttact tgctgtacat aaaaaaggaa aaccacccat ggagctcatt
     5821 gttccaggtg gtgttatgga ggaaaatgaa agcgatgaag agaccctgcg tcgagaaatc
     5881 aaagaagaac ttgactccga tatcatatca tttcagtttt ataagcaatt tgaagacaag
     5941 gcaatctatg aggaaaaatg gctggtaatg cgtacttaca ttgtcactct cggcaatgcc
     6001 cctaaacctg ctaatgagat tgatcaactg gtctggcttg ggcatgatta tcatcaaagt
     6061 ggctatcgtt ttggatccat attaggcaaa cagattattc ctgatttctt taaataaacc
     6121 attcccagac attcacagca acttttggtt cagaccagga agtaaagtcc gaacctttat
     6181 gagatgagga taaaatgatg ctatttggtt aaacaaggca ctaatccgaa ttgttgatca
     6241 tgaatatcct gctcagcatc tgaacaatgc cgagcttcag tacttacctt tggagatgac
     6301 tctttaaaaa aacccaacct ttttccagaa agaccttctt gacagatttt atccgccatc
     6361 attttacgat tgatctgact ggctaatttc gtttcctcaa ttaaaaaaat tgtaaacact
     6421 ttggtaggaa cttgagttac tctttgtatg tcacgctgag gtgtatcttg cttcaaaaga
     6481 acagagtcag ccaattctgt ctcagttaat gcaatggcat aagggcctac aaaaaaagag
     6541 tcgccatgga ttacattact ggcaattttc atggtatagc caaatggtat ttcaaacgca
     6601 gcgaaatcaa atgccccatc tttgcgctcg actcccagaa tcaatgcacc acgacatgca
     6661 ggggataagg gcgtaaaaac atgaggaaat gggtgtgttt ccacgaacaa tccaccacca
     6721 cctcttatat tcattacgta atcttgaata taatcagcat cccattgata ctcaaccaga
     6781 tacaggtttt ccagtgacga cttatcaaca gtatccaggg taaatggctg atctagggga
     6841 atcagctcgg caccatattt ggctaaaaat gatgcaggca cagccaatgc attcattttg
     6901 gggatattca ttactttcgt atcaggagag ataaccaata ctcccaagcc accaaaaacg
     6961 ctggcatccc tcccactgat tcctctttcc tggtttttat ttttccaggt tgctttcatg
     7021 gttaattctt ccggcgcagt catgggaata gcaccttttg aagaaggtaa atcatcttca
     7081 cttattccag tagcagttaa catatcgatt tctgtgcctg ttttaagaat ggcatgagcc
     7141 tttgccgact tcattaggga cttactatca gacagatcgg gatttcgtcg gtcaaccatc
     7201 gtggattgca aaccatgtaa atgctttgct accacatcgg ctggcaattt gcttttcatg
     7261 catatggaag aagtgcatat ttcattactt ttactggaag gagttattcc ggatcccgga
     7321 ttgttttggg cagttttatc gaagagtact ttcataatta taaccacaca tcttatagat
     7381 taaaatatga ctcaattgat ctattaaaat agataaagtt taaattatga actaaaaata
     7441 ttaagatttt attaaattaa gaactatatg ctcaaatata atcaataatt ttattctaat
     7501 atgatatt
//
LOCUS       DAXLDT010000141         7422 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXLDT010000141 DAXLDT010000000
VERSION     DAXLDT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.141"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(117..1361)
                     /gene="mavL"
                     /locus_tag="ACF2SP_002453"
     CDS             complement(117..1361)
                     /gene="mavL"
                     /locus_tag="ACF2SP_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavL"
                     /protein_id="HFD2360227.1"
                     /translation="MAYQLLLSKETLNKILQYKQNLEKGLAMPGKFFLEELSKQEKSI
                     SEMDITTFTQLLIQSKKPQVFAESQVYHDGTDWTLEEESILGDVSVNMPVTMYNDGGH
                     GSSFKNHPKPISGYLAYVPGALLASGSGPTSDMKEVLDNGKLNQDKLNALYERRLLPQ
                     LIHFNELARQNEKQAAITIPGIGTGCFSGAYYDVIKPYVRNALIHILEKHKDSLPYID
                     IIHYDPYVGDEPAEKKIGHMSFRVSPSGVVRGTTGQLDYPLGSNPDTHILVSIVAWDH
                     FSWPGNDYWGGARQTDDGVKAASTDTMGQVTGATGVYDKKWGRYMPPESFTKDRKGMS
                     DWGDYVRENGIVFNGPVLALDKSGKLDTLENVASRSKAKVDTTATISDLVRGMFSLFS
                     HSTTTELSPTVKEEESKKSGPQ"
     gene            1680..1832
                     /locus_tag="ACF2SP_002454"
     CDS             1680..1832
                     /locus_tag="ACF2SP_002454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360228.1"
                     /translation="MLISLLAQADYFGLGQGANVLMSEIYIQALHKHLQRGNKMKGVG
                     YATALL"
     gene            complement(1824..3497)
                     /locus_tag="ACF2SP_002455"
     CDS             complement(1824..3497)
                     /locus_tag="ACF2SP_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2527 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360229.1"
                     /translation="MLYFVLCDLKINQFSRYPMSYQKIELNISLSLDSPKLDESDFIL
                     LSVKYLEKSLGKKKEFSVFFEDIEKLYFKQNYKEAIEKILDFCKKNESLLSEQVVKRL
                     AEVAPRLKSNPKDNESRRLYETLYADHLESVIKQESDLSVFNELRDSYNAVKPEYAVT
                     HETEIKTLDEAKQFILSFVMLNDNVELPLKAQSERYPKKDRSREELGNTPSANPGIMK
                     PNSPNFTDNLVPVRDVPKIAINEKVVGGYSKTKPTTPFVASLSGTTYSLMVVLTDYIE
                     KHKTDKDIEKKVNQIINLWISSYIKEGYHSYSEVVDVLTEPFLQSIFDKANIKLNYGV
                     LDDTHAEFRKAQDYVFGLTIRSAMHHELQERFKNKEKLQEEVKNFESGLNKLNQNVEN
                     TGRHREELNKLNEVFQDWSAGKKSYDAFKSESNQLIHDIESEEQKSNRGLGSMLKNFG
                     NYLLYLITLRFLKEDYPKPTSPVTMLVTELRGTLEQIDHFHKTSLSLKKATQPGPKNT
                     SPSGETTDALKSAVDTDKHDKNQESEVIKTLDSEQLEEERQDDKIARLQ"
     gene            3664..5214
                     /locus_tag="ACF2SP_002456"
     CDS             3664..5214
                     /locus_tag="ACF2SP_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFD2360230.1"
                     /translation="MSPRICCYNQYPGLYQDFDAMREDLQRIQKMGFKQVWVNPFYTP
                     CQYNPVPNMANRIHSPYAMQDESIYPRYAKNEKSVQQYTAKANELGLMPVFDLVARHL
                     AVDHRFVNGDKFFLEKYNIDTKKWFKRHPNGNLVMHNMDENYNPLTNNPWSDVATFNY
                     DDPVICEQIIEYYWKPFIERNIEHLGFQGIRIDAPAMVNNKVLSRLTEIAKEACQRKF
                     QRDPLIIAETIGRGYMEENLALKGIVTHTMNSVFWMPGPEGGYGYSFDLWQQDDNWFT
                     QNKGLLQQVGPTIGFPGSHDEPRYIQQLVEKGIHDDKLLAKRMREKLAVSAFCSDGGW
                     ILQYGDEYGATKPVNVFDPTPVEYHQQNHLRRFDLSDYISEINTTLAQLPNPHFPEWA
                     QRVFLPNHPDLVTFIVHQGWGYTGTSFVIATNTDPSQQIQLTEKELGEIMLANGRNNT
                     TEKQIKPQVLYLCGAVRASPELKKEIQVVASRPTASEKSPQFFQSHPGEKKTGEKLHS
                     DNTPPPFK"
     gene            complement(5615..7345)
                     /gene="lem27"
                     /locus_tag="ACF2SP_002457"
     CDS             complement(5615..7345)
                     /gene="lem27"
                     /locus_tag="ACF2SP_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem27"
                     /protein_id="HFD2360231.1"
                     /translation="MVRVLNLQKGVNMFNVDNSGKGNCLYYAYSISLMYYLRAKNNVR
                     ITEDIFNKLGLKEEDRARLRKLLSKDPDRAFTRDEIKTIIEPILGRATRDLAAEHTKV
                     EFKSSPHDTPLFSSLHYAVEFGFKRSLQINESELTLLIDNDFSNPDYTEAEIYKVSGL
                     LDALQEYILTRTPSVIEEFNRQWENKKQELKEQSLTEKEIQIHQATILDNILRKETID
                     FLLADNEKHLDEYREHLRREFVWGSEETLMVLHRAIQGERMVRNHEGRIEPVYDHEII
                     LHVHRNGASPSYQAGSPEMILNNEGNVHWTSIIPDAIFTSKLTDKEEKLLEMLERMQS
                     EYGSKELGVEKYSSISDWLSDLMKQIELIKTSPTAVTKQKEIELFFQLLAKATPKLAS
                     EPALRMSLGTLFSNFLECIPALMVENKLSTGLAKFGLYSPERSPVISQKKEDLSLTKS
                     SEEKVNSDQSQKGVLSQFSQHTLLKVSEEITPKPYPTELNPYVRKNKQSGPRVARALT
                     QMYQKGLEGDERYTPEKCKDIAQRYIEYSKSHKRNFILGDVILFVQEMDKIIKQKETI
                     SEVTCKLVAM"
BASE COUNT         2179 a         1483 c         1337 g         2423 t
ORIGIN      
        1 tatctcagtt aaaaattaga ttaattgggt tgtctcgttt tgttttacca cggcatgatg
       61 tgaaggcctg accagaaaag tcaggccttg tttttacctg tcaaactata ttattactac
      121 tgaggacccg attttttcga ttcttcctct ttaacagttg gagaaagctc tgtcgtagta
      181 ctatgggaaa aaaggctgaa catgccacga acgagatcac taattgtggc cgtagtatca
      241 acttttgctt tggaacgact tgcaacattt tccaaggtat ccaatttacc gcttttatcc
      301 agagctaaaa cagggccatt aaatacaatg ccattttctc tgacataatc cccccagtca
      361 ctcatacctt tacggtcttt agtaaatgat tctggaggca tatatcgacc ccatttttta
      421 tcatagacac cagttgcgcc tgtaacttgc cccatggtat ccgtgctagc ggccttgaca
      481 ccatcatctg tttgacgagc gccgccccaa taatcatttc cgggccagga aaaatgatcc
      541 caggcaacaa ttgaaacgag gatatgcgta tctggattac tacctaaggg gtagtcaagt
      601 tgccccgttg tgccacgaac cacaccagaa ggactcacac gaaaagacat atgaccaatc
      661 ttcttttcag caggttcatc tcccacataa gggtcataat gaatgatatc aatgtaaggt
      721 aacgaatctt tatgtttctc aagaatatgg attaatgcgt ttctgacata gggtttaatc
      781 acatcataat aagcgcctga aaaacaccct gttcctatac cgggaatagt aattgctgcc
      841 tgcttttcat tttgccgcgc caactcatta aaatgaatca gctgaggtaa caaacgtctc
      901 tcgtacaaag cgtttaattt gtcttgattt agtttgccat tgtctagcac ttctttcata
      961 tctgaagtcg gcccactgcc cgaagccaag agggcacctg gcacataagc caaataaccg
     1021 gaaattggct ttgggtgatt cttgaaagag gagccatgtc caccatcatt gtacatagtg
     1081 actggcatat tgacactaac atccccaaga atgctctctt cttccagtgt ccagtcagtt
     1141 ccatcatgat aaacctgact ttcagcaaaa acctgtggtt ttttagactg gattaacagt
     1201 tgagtaaatg tagtaatatc catttcagag atgctttttt cttgtttact taattcttca
     1261 agaaaaaatt taccgggcat ggcaagccct ttttcgaggt tctgcttgta ttgtaaaatt
     1321 ttattcaggg tttctttact gagcaataat tgataggcca ttatattctc acttaatcca
     1381 aaaattgtat taatgtttct tactacgaag tccttacaat aagcatgctt actggaaatc
     1441 ttcccctaca gatgtgttga ttgtactgaa acaatctcaa acctattgcc tcaacaaaag
     1501 gaatttaatt agtcaatatg actataatga taacacataa tgactgctat tccaatcagt
     1561 gaaaacctga tatctcgttt tcaaaattgg gatatggctt gataaatata taactgccta
     1621 gttttattgc aaatactttt ttagtcattt ataaattttt aattaaatgc atataatcca
     1681 tgctcattag ccttcttgcc caagctgatt attttggttt agggcaaggt gcaaacgttt
     1741 tgatgagtga aatttacata caagcacttc ataaacattt acaacgcggc aataaaatga
     1801 aaggagtggg ttatgcaaca gctctactgt aaccgagcta ttttgtcatc ttgcctttct
     1861 tcctccagtt gctcagagtc taatgtcttt attacttcac tttcctggtt tttatcatgc
     1921 ttatcagtat cgacagctga tttcagagcg tctgttgtct caccagatgg cgaagtattt
     1981 ttagggccag gctgcgttgc tttttttaaa gagaggctgg ttttatgaaa atgatctatt
     2041 tgctcaagtg tgcctcttaa ttcagtgact aacattgtca ccggactagt cggttttgga
     2101 tagtcttctt tcaaaaaacg aagggtaatc aagtataaaa gatagtttcc gaagttcttc
     2161 agcatagagc caagaccacg attacttttt tgttcttcag actcaatatc atgaatcagt
     2221 tgattagact cacttttaaa agcatcatag ctttttttgc ccgcagacca atcctgaaat
     2281 acctcgttta atttattcag ttcctcgcga tggcgacctg tattttcgac attctggttg
     2341 agtttattta acccagattc aaaattcttt acctcttcct gcagtttttc tttattctta
     2401 aatcgctctt gtaactcatg atgcattgct gatcgaattg tcaaaccaaa aacataatcc
     2461 tgagctttac gaaattctgc atgcgtgtcg tcaagtacac cataatttaa tttaatattt
     2521 gctttatcaa aaatggattg cagaaacggc tctgtcaaaa catccacaac ctcactgtag
     2581 ctgtgataac cttccttgat ataactggaa atccataaat taataatctg gtttactttt
     2641 ttttcaatgt ctttatctgt tttatgcttt tcaatatagt ccgttaacac caccataagt
     2701 gagtaggttg tgccagacag cgacgccaca aaaggagtag taggcttggt ttttgaatac
     2761 ccacccacca ctttctcatt aatggctatt ttgggcacat ccctgacagg caccaggtta
     2821 tcggtaaaat taggtgagtt aggtttcata atccctggat tcgcagatgg agtattcccc
     2881 aattcttccc tggatctgtc cttttttggg tatctctctg attgtgcttt taaaggtaat
     2941 tcaacattat cattcagcat gacaaaagat aaaataaatt gctttgcttc atccaaagtt
     3001 ttgatttcgg tctcatgagt tactgcatac tcaggtttca ccgcattata actgtctctc
     3061 aattcgttaa atacggaaag atcactctct tgtttaatga ctgattcaag gtgatctgca
     3121 tacaaggttt cataaagcct tctgctttca ttgtctttag gattgctttt taatctggga
     3181 gcgacctcag ccagcctctt aacaacttgc tcacttaaca aggattcatt ttttttacaa
     3241 aaatccaata ttttttcaat tgcttccttg taattctgct taaaatagag cttttctatg
     3301 tcttcaaaaa aaacagaaaa ctctttcttt ttgccaagtg atttttcaag atacttaact
     3361 gagagcaata taaaatcaga ttcatctaat tttggagaat ctaatgataa tgaaatattg
     3421 agttctattt tttggtatga cataggatat ctgctaaatt gattaatttt taaatcacat
     3481 agtacaaaat ataacaaaat gtttaataaa agtaaatatg atacccataa agaacaaaca
     3541 acacactttg gttaattttc taagcatgca taggaattaa atggttttat aaaatgcaat
     3601 gaggtctaat ttaatacttt tactatactt catatacata gaaacagaaa ttgagaagtc
     3661 agcatgtctc cacgtatttg ttgctataac caataccctg gcttatatca agattttgat
     3721 gcgatgcggg aagatttgca gcgaattcag aagatgggtt ttaagcaagt ttgggtcaac
     3781 ccattttata caccttgtca atataatcct gtacctaaca tggctaatcg tattcatagc
     3841 ccttatgcca tgcaggatga atcaatttat ccacgctatg ccaagaatga aaaaagcgtt
     3901 cagcaataca ctgcaaaagc aaatgagctt ggtttaatgc ctgtatttga tttggtcgca
     3961 aggcacctgg cggtagatca tcgttttgtg aatggcgata aattttttct tgaaaaatac
     4021 aatatcgata caaaaaaatg gtttaaacgt caccccaatg gaaatttggt gatgcataac
     4081 atggacgaaa attataatcc gttgaccaat aatccctgga gcgacgtggc aacgtttaac
     4141 tatgatgatc ctgtcatttg cgaacaaatt atcgaatatt attggaaacc atttattgag
     4201 cgtaatattg agcatcttgg ttttcaggga atacgtattg atgctccagc catggtaaat
     4261 aacaaggtat tgtctcgact aacagaaatc gcaaaggagg cttgccaacg caaatttcaa
     4321 cgtgacccac ttattattgc agaaacgata ggcagggggt atatggaaga aaatcttgct
     4381 ttgaaaggta ttgtaaccca cacgatgaat agtgtgttct ggatgcctgg ccccgaaggt
     4441 ggctatggtt attcatttga cttgtggcaa caggatgaca attggtttac tcaaaataaa
     4501 ggattattac agcaggttgg tcctacaatt ggttttcctg gctctcatga tgagccacgc
     4561 tatattcagc aactggtgga aaaaggtatc catgatgata aattgcttgc aaaacgcatg
     4621 cgcgaaaaac tggctgtcag cgcattttgt tctgatggcg gttggatttt acaatacggt
     4681 gatgaatatg gtgctaccaa gccagtaaac gtttttgatc caacccctgt tgaatatcat
     4741 caacaaaacc atctgagacg atttgatctg tcagactata tctctgaaat taatacaaca
     4801 ctggcccagc tacccaatcc tcacttcccc gaatgggcgc agcgggtctt tcttccgaat
     4861 catccagatt tggtaacctt tattgttcac cagggttggg gttatactgg gacctcattc
     4921 gttattgcga caaacaccga tccctctcag caaatccaat taacagaaaa agaacttgga
     4981 gaaataatgc tggcaaatgg ccgaaataat acgactgaaa aacagataaa accacaagtg
     5041 ctttatttat gtggcgcagt tcgcgcatca ccagaattga aaaaagagat ccaagttgtt
     5101 gcatcaaggc caactgcaag tgaaaaatcc ccccaatttt tccagagcca tcctggagaa
     5161 aaaaaaacgg gcgaaaagtt gcattcggac aatacaccgc ctccatttaa ataacaggta
     5221 ccaggcttag ccctaaacca tgcttactgg tgagttgatt attatcatat tgcttactca
     5281 ccaatcagga catcactttg acaggactta cgaaaaactc caagatcaag gcaaaaaatg
     5341 tttttaatga gggagtttag ataaactaaa tgactgaatt aaaaacattt tttaacatag
     5401 agattggggt ttttcgtaag tcctgtttga taaggaagca gtatggcttt gatgatctta
     5461 tcgttcctgc ttaaaaaaaa gcaggaacct ctctcttgca taaactgatt attttgtttt
     5521 agagcaaggc gcaaacgttt tgaggagcag tgtttacatc cagtaaataa gcacctcaga
     5581 aacgtttgaa aagcgacagg ttgtgtcagt tttcctacat tgctacaagt ttacaggtaa
     5641 cttcgctaat tgtttctttt tgcttaatga ttttatccat ttcttgcaca aagaggatga
     5701 catcaccaag aatgaaattg cgtttatgag atttcgaata ttctatatac ctctgagcaa
     5761 tgtctttaca cttctccggg gtatatcttt cgtcgccctc aagtcctttt tggtacatct
     5821 gtgtgagtgc tctcgctacg cgtggaccag actgcttgtt ttttcgaaca taagggttta
     5881 gttctgtggg gtatggtttt ggggttatct cttctgatac cttaagcagg gtatgttgcg
     5941 aaaattgact taataccccc ttttgagatt ggtcagaatt gactttctcc tcagatgatt
     6001 tagtcaagga caagtcctct tttttctgag atattacagg actgcgttcc ggactgtaca
     6061 atccaaattt agctaagcct gtgctgagtt tattttcaac cataagggct ggaatacatt
     6121 cgagaaaatt ggaaaaaaga gtaccaagcg acatcctcaa ggcaggttcc gatgctaatt
     6181 taggtgttgc tttagccaga agctgaaaaa ataattcaat ttctttttgt tttgtcacag
     6241 cagttgggct ggttttaata agctctatct gcttcatcaa atcgctaagc caatcactta
     6301 tcgaagagta tttttctacc ccaagctctt tgctgccata ctctgactgc atccgctcca
     6361 gcatttcgag tagtttttct tctttgtctg ttagctttga ggtaaatatc gcatcaggaa
     6421 tgatagaagt ccaatgaaca ttgccttcat tatttaatat catttccgga ctgcctgcct
     6481 gataggacgg acttgctcca tttctatgaa catgcaaaat aatttcatga tcatagacag
     6541 gctcaattct tccctcatgg ttacgtacca tgcgttcacc ttgtatggcg cgatgtaaaa
     6601 ccatcagtgt ttcttcacta ccccatacga actctcttcg aagatgctct ctgtattcat
     6661 caagatgttt ttcattatca gctaataaaa aatctatcgt ttcttttctt aggatattat
     6721 ctaaaatagt cgcctgatga atctggatct ctttttcagt taagctttgc tctttgagtt
     6781 cctgtttttt attttcccat tggcgattga attcttctat gacggaaggt gttcttgtta
     6841 aaatatactc ctgaagagca tccaacaaac cacttacctt gtatatctcg gcctcagtat
     6901 aatcaggatt cgaaaaatca ttgtctatta acagcgtcaa ctcggattca ttgatttgta
     6961 gtgagcgttt aaaaccaaat tccacagcat aatggagaga tgaaaataaa ggcgtatcat
     7021 gaggtgaaga tttgaattcg actttggtat gttccgctgc caaatctctc gttgccctgc
     7081 caagaatggg ttctatgatt gttttgattt catcgcgggt aaacgccctg tcagggtctt
     7141 tggataatag ttttcgtaaa cgggccctat cttcttcctt taatcctaat ttgttaaaaa
     7201 tatcttcagt aatcctgaca ttattttttg cccgcaaata atacattaat gaaatactgt
     7261 aagcataata caagcaattg cctttaccac tattatccac attaaacata ttgactcctt
     7321 tttgtaaatt caaaaccctc accacattaa tagtctttta ttgctcttcc tccttggttt
     7381 gatttcatta tttgaaagat tcatgagttc ttttctggta at
//
LOCUS       DAXLDT010000142         7406 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXLDT010000142 DAXLDT010000000
VERSION     DAXLDT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.142"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(115..1338)
                     /locus_tag="ACF2SP_002458"
     CDS             complement(115..1338)
                     /locus_tag="ACF2SP_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2223 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360232.1"
                     /translation="MIDEKNYFLKLDFARQRVIDLPAGEFKYYLYILLESIKSQNVPS
                     RHEFLAVLLKLLDTLHKLKELKTPNFLDNQCPSSQLLKELALHLCELQRVAKTHHFAA
                     QVMIILLHIGGIISAVALSVVGGLLGGILGFVNGLCEYKPVIGFLVGTFTGTVLGGLF
                     GFRAPKKLFKNEVQRQIKFGLDGLGESLDNINQEYSSSMFGPNKIKPFSFYLDQVETE
                     IKQLFNDDSEYEEFLNHKIDYEINSFLASFIGESLLHGCVGHHAYIKTNIKNREYLIE
                     FTPQPTDTKEDPAQSETRTVSGKKIVEMLAYHKKLQETNPPTASFILTKMKPGDNDCF
                     SYVNKILIGTNQQGATIKRFANMGLIGSLLGIGIEALSPFKPDFFQTTSSVRFHKEKM
                     PDRSNDLSMNSLLPQ"
     gene            complement(1547..3076)
                     /locus_tag="ACF2SP_002459"
     CDS             complement(1547..3076)
                     /locus_tag="ACF2SP_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HFD2360233.1"
                     /translation="MNWKRFLIAAMTKKKYQVFLNDTKHPKEARERLWNSEIVPLLKK
                     SSYWQKLLKDQSSVALNDFGITEYEDYQEGLLAAQHSTIQPFNGEELIFWSETSGTSG
                     VRKFFPITDSFQKQFQRTMAPYIYSLTQRFPGFFKEKMLYLVAVDTHKTTPAGIPTGW
                     ISNFNYRHLPSFIKKFYAMPDEVFASHEAYNQWGPIYGLASDLSAIFAVTPMVIDAFY
                     QRCIDGFKEYLPYLLGNKPLPSHLPPLNMTRKRRRHLQALAKVDHLSFKQLWPSLEMA
                     GCWTSGLCEYPAQQLQKLLGSEIKLVDGTYSATEGWLTVPVETNSVGGILHPGAHIVE
                     FIPEGAAIEKENLLQCWELEVGKKYEVFLTTAMGFIRYRLKDIVKCTGYLNSSPKLEF
                     CYKTQLLKLETCSITGSELQSAVLATPLNIEPYWYFARNSVGSRIVLVTDDEADISEL
                     SLKKLHEQLIHISPTYAHSVETEEVLPVTLLQLPKEKLLAGMHGQTKPKFISQEVIAE
                     R"
     gene            complement(3083..4774)
                     /locus_tag="ACF2SP_002460"
     CDS             complement(3083..4774)
                     /locus_tag="ACF2SP_002460"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFD2360234.1"
                     /translation="MTSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQEILAS
                     PAIEFIKQWGYLDYTVPHYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFTALPI
                     WIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCV
                     NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFS
                     FDKVKVSKDALIGKEQRGLEITYKVFQISRTLCACLAIGGADTALRLALSYSLQRQLY
                     GKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPEKMSLWSAIIKFLIPHIGE
                     DIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGM
                     RGKHRAEHSEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITT
                     IRHEIEKLDQEVIRLHDEKLFDPRSLAVFRLAEKYCWIFAASCCLQFWHYNQEILSNN
                     LQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIHIAG"
     gene            complement(4783..6204)
                     /locus_tag="ACF2SP_002461"
     CDS             complement(4783..6204)
                     /locus_tag="ACF2SP_002461"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HFD2360235.1"
                     /translation="MSSQHTDKTLIKWIDNLIDEQSFLPIGSEYIPDNTPHSLPGAEV
                     ITGLAKVNQRPVAIYAHDSSVNRGYVTRQGARKIIHLMDKAEKLRIPIVAFLASPGVS
                     LEENLLSGDEYTQIIARNIRLSGIIPQFAVILAPTLGAPAYSAVLMDLLFFNKHRSYL
                     MVTSPTVVQQAIGEKVTMGELGSATLHATTTGIADFVDDSVTAQINHVKAILGFFPLH
                     TNERPPVHPIMEPNHPIPDIPANPRMPFNMLNLIQAVVDNSNVIQYKRTYGQAMICAF
                     VHIHGFAVGVVANQSIRYSGAIDSDAAQKAAKFIRICDAYSIPILTFIDVPGFMPGKR
                     EEQKGLLQHGARFCVAMQTRVPRLSVIVRKCYGAAAFLMMQTKSQNGDLVLALETANL
                     GVMGKETTNKVQAMDGQPVQESSQSQLTEKSQIDDSLMEAYSLGLIDEIIQAAQIRSR
                     LASHLEFLYRRMETMPPAKHSIV"
     gene            complement(6426..7289)
                     /locus_tag="ACF2SP_002462"
     CDS             complement(6426..7289)
                     /locus_tag="ACF2SP_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFD2360236.1"
                     /translation="MKFYHSALVSFAFLSLAYNAKAGTLGIAPSMDFYANAGLGAVFS
                     NTSTSNTSNSNSVLYTPTAIGTSLFTLPNINWQNKFKNGYGINLAVGKYFSSNWHGDL
                     EFLYQNIERDTYGTYNWLEQNSTTGAAYAQQANNLISKISTRANIYSFMTNASYDLMN
                     VGKLQPFLGAGLGLAWLRSSSTQTNNSININDPNTPLVETAPAMQNSPSLYGTAFAWQ
                     FKAGINYAVSEKATAILQYRIFKTTDFKASQSTIITNPGMAGQTIFYAGQHDINGLLT
                     HAVELNLRFDV"
BASE COUNT         2295 a         1568 c         1320 g         2223 t
ORIGIN      
        1 tagggaaagg tacatacgtt ttgaggagcg gagtttatat gcagtaaatg cgcacaaagt
       61 ggcaataaca tgaaaaagac agattatctt tgcaatccga gattggaggg ctggttattg
      121 aggtagcagg ctattcattg acaaatcatt cgaacgatca ggcatttttt ccttatgaaa
      181 tctcactgaa cttgtagttt gaaaaaaatc tggtttaaaa ggacttaaag cctctatccc
      241 tattcctaat aaagaaccaa tgagccccat attggcaaat cgcttgattg tagcgccctg
      301 ttgatttgtt ccaatgagta ttttattgac gtaactgaaa cagtcattat caccgggttt
      361 cattttagtt aatatgaaag aagctgtcgg tggattcgtt tcctgaagtt ttttatgata
      421 agccagcatt tctacaatct ttttaccact tactgttctt gtttcgcttt gagctggatc
      481 ttctttggta tcggttggct gaggggtaaa ttctattaaa tactcacgat ttttaatatt
      541 agtctttata tacgcatggt gacctacaca cccatgaagc aaggattctc caataaaact
      601 ggctagaaag gagttaattt cataatcgat cttatgattt agaaattcct catactcgct
      661 atcatcattg aaaagctgct taatttcagt ttctacttgg tcaagataaa atgagaatgg
      721 tttaatttta tttggaccaa acattgatga ggaatattcc tgattgatgt tgtccaagct
      781 ttcacctaaa ccatcaagac caaattttat ttgccgctgt acctcattct taaatagctt
      841 tttcggtgcc ctgaaaccaa aaagcccacc aagaactgta ccggtaaatg ttccaacaag
      901 aaaacctata accggtttgt attcacataa accgttaaca aaacccagta ttcctcctaa
      961 caatccaccg actacgctta aagctacagc cgaaataata ccgccaatat gcagtaaaat
     1021 aatcatcact tgagcagcga aatgatgagt cttggccact cgttgcaatt cacagaggtg
     1081 aagcgctaat tctttcagca attggcttga tgggcattga ttatctaaaa aattgggagt
     1141 ttttaattct tttagtttat gcaaggtatc cagcaatttt agcagtactg ctaaaaattc
     1201 gtgcctggat gggacatttt gggattttat cgactctagc agaatgtata gataatattt
     1261 aaactctcct gcaggcaagt cgataacacg ctgtcgggca aaatctaact ttaagaaata
     1321 atttttttca tctatcatga aataacaaac cagcagggac ccataacgga aaacatatgg
     1381 cccaaaatga tactataata aggctatttg atcaatcatg gggcaataat tcagaaaaca
     1441 acgctcattg ccattattga taactatcca tcacagccaa ttaaaatagg ccaaaatcta
     1501 tctatattga tacgattggc aagaaatgaa tttgtcagcg ggtgcttcat ctctcagcaa
     1561 taacctcctg agagataaat ttcggtttgg tttgcccatg catgccagct aataattttt
     1621 ccttgggcaa ttgcagcaag gtcacaggta aaacttcttc ggtttccaca ctatgagcat
     1681 aagtaggact aatatgaatt aattgttcat gcaatttttt cagtgatagc tctgaaatat
     1741 ctgcctcatc atcagtcacc aaaacaatac gactcccaac actattgcgc gcaaaatacc
     1801 aataaggctc tatattgagc ggagttgcca gcaccgcact ttgcagttca ctacctgtaa
     1861 ttgagcaggt ttccaatttc aagagttgag ttttataaca aaactcaagc tttggcgaac
     1921 tgttcaaata tccagtacat ttcacaatat ctttgagtct gtagcgaata aaccccatag
     1981 ctgttgtcaa aaatacttcg tatttcttcc ctacttccaa ctcccaacat tgtaaaagat
     2041 tctctttttc aattgccgcc ccctctggaa taaactccac aatatgggca cctggatgta
     2101 aaattccacc cacgctgtta gtctctaccg gaaccgttaa ccacccctcg gttgccgaat
     2161 aagtaccgtc taccagttta atctcggaac ccagcaattt ttgtaattgc tgagcgggat
     2221 actcacaaag accagaggtc cagcatccag ccatttccag agatggccac agttgtttga
     2281 atgagagatg gtcaaccttg gctaaagcct gcaaatgtct tcgtcgtttc cgggtcatat
     2341 tgagaggtgg aagatgtgat ggcagaggtt tattccccaa taaataagga agatattctt
     2401 taaaaccatc aatacagcgt tgataaaaag catcaattac catcggcgtc accgcaaata
     2461 tcgcacttaa atcagaagcc aaaccgtaga tgggacccca ttgattgtaa gcctcatggc
     2521 tagcaaaaac ctcatcaggc atggcataaa attttttaat aaaggaaggc aaatgacgat
     2581 aattaaaatt actaatccat cccgtaggaa taccggcagg agtcgtttta tgagtatcta
     2641 ccgcgacaag atacaacatt ttttccttaa aaaagccggg gaatcgctga gttaggctgt
     2701 aaatatacgg ggccattgta cgctgaaatt gtttctggaa tgaatctgtt atagggaaaa
     2761 actttctaac tcctgatgtt cctgaagttt ctgaccaaaa aatcaattcc tcaccattaa
     2821 aaggttggat agtagaatgc tgggctgcca acaatccttc ctgataatct tcatattctg
     2881 taattccaaa gtcattaaga gctacagaag attgatcttt taatagtttc tgccaataag
     2941 atgatttttt cagcaacggg acaatttctg aattccataa tcgttctctc gcctctttag
     3001 gatgcttggt atcattcaga aaaacctggt attttttctt tgtcatggcg gcaattaaaa
     3061 atctcttcca attcatccct cattaccccg caatatgtat tggcataata gaaaacagct
     3121 gtttttcctg ataaaaagaa tacaaacgct cactcatagc ttcttgcaac acgctatcaa
     3181 tcatcgaacc tgaattcaac cgcttaagaa ttaactgcat cgctaaatcc agccaatctg
     3241 tattttgcaa attattagaa agtatctcct gattatagtg ccagaactgc aaacaacaac
     3301 tggcagcaaa aatccagcaa tatttttctg ctaaacgaaa aacggctaaa ctgcgtgggt
     3361 caaataattt ctcatcatgt aaacggatga cttcttgatc caatttttca atttcatgac
     3421 gtattgtagt gatcaatgga ttaattggaa ttgagtttaa cgataggata ccagccaata
     3481 catcatcttc ttcatgagta aacaaaccga gtctatcctc cttaaaagga ggacaagaca
     3541 aacgaacatt aaaaattttc tcaatttttt cggaatgctc tgcccgatgt ttaccccgca
     3601 tgcctgcctg aggcaacaaa ttaccagcaa taagtgataa attaacttga gtgcttccat
     3661 caaacaagcc aaccacttga atgtcgcgtc ttattttctg aaaaatagcc cattgcgtgg
     3721 ttctcagata agctctggcg cctagaataa tggcacactg ttctgctata tcttctccaa
     3781 tatgcggaat aaggaattta ataattgctg accaaagact cattttttcc ggtattaccg
     3841 tacaggcgcg aaccataacc tgagcagtgc aatccgcaat taataaaaga gtaaacaatt
     3901 cgccaagccg ctgttttact gcgggtattt caaacactga tttgccatac agttgtcttt
     3961 gcaagctgta tgataatgcc agcctcaaag cagtatcagc gcccccaata gccaaacagg
     4021 cgcataaggt tcgcgaaatc tggaatacct tataggttat ttccaaacca cgttgttcct
     4081 tccctattaa tgcgtcttta gatactttca ctttatcaaa agaaaaacca ctgatatcta
     4141 accctctgac tccatgagtt ggcagcttag gcgtaggaga aatccccttt tcagctaatt
     4201 ttttatcgat ataaaacaaa gaaaaaccta ataacccgcc cttttcatta gtacgacaca
     4261 acaccacagc gtactcgctc aaggttgcaa aattaacaca ccattttcgc cctgaaattt
     4321 cccagccatc ttcagttggc ttggcattca tcttattgga agtcaaatcg gttccatgct
     4381 cttcttcagt caaagccaaa gcacctatct ttccctggcg aaggctctca ctcaaatgtt
     4441 tcttttgctt tgagtttcca gctatccata ttggcaatgc agtaaagaaa gccaatccaa
     4501 acataatagc catcgtgatg tcacgcctgg ccaacaacct ggttaaagta tacagctcat
     4561 ccagggagct cagcttaccc cctaaataat gaggtacggt atagtccaaa tacccccatt
     4621 gtttaataaa ttctattgct ggcgaagcta atatttcctg ctcatcagcg agcaaaattt
     4681 gttgaaaatt aacaggggaa ttgtcttcca gaggattacc caggcatgac tcaaaaaatt
     4741 cagacagttg tatcaaattt tcataagagg tcattatttt ccttatacaa tagagtgttt
     4801 tgccgggggc atggtttcca ttcttctata taaaaactcc aaatgagaag caaggcgact
     4861 gcgaatttgc gctgcttgga taatttcatc aatcaagccc aatgaataag cttccatgag
     4921 ggaatcatca atttgagact tttctgtcaa ttgggattgt gatgattcct gtactggttg
     4981 accatccatg gcttgcacct tattagttgt ttccttcccc atgacaccaa gatttgctgt
     5041 ttccagagcc aaaaccaaat ctccattctg tgatttggtt tgcatcatga gaaaagctgc
     5101 cgctccataa catttacgca cgatgacact taaccttggc actctggttt gcatagctac
     5161 gcaaaacctt gcaccatgct gcaataatcc cttttgctcc tctcttttac ctggcataaa
     5221 acccggaaca tcgataaaag tcaaaatcgg tatagaataa gcatcacata ttcttataaa
     5281 tttggctgct ttttgagccg catcggaatc gatggctccg ctgtaacgaa tgctttgatt
     5341 agcgacaacc ccaacagcaa agccgtgtat atgcacaaaa gcacaaatca tcgcctgtcc
     5401 ataagtcctt ttgtactgga taacgtttga attatccacg acagcctgaa ttaaattgag
     5461 catattgaat ggcattctcg gatttgcagg aatatcagga atggggtgat taggctccat
     5521 gataggatga acaggcggac gttcattggt atgtagagga aaaaaaccaa gtatcgcttt
     5581 cacatggttt atttgtgcgg taacactatc gtccacgaaa tcagcaatac ccgttgtcgt
     5641 tgcatgcagt gttgcgctcc ccagctcgcc catagtcact ttttcaccaa tggcttgttg
     5701 tactaccgta gggctagtca ccataagata gcttctgtgc ttgttaaaaa ataataagtc
     5761 cattaacacg gcggaatatg ccggcgctcc aagagtgggt gccagtatca ctgcaaattg
     5821 tgggataatt cctgacagtc taatatttcg agcaataatt tgagtgtatt cgtcgccact
     5881 cagcaaattt tcttcgagag aaaccccggg agatgctaaa aaggcaacga tgggaatgcg
     5941 taatttttcc gctttatcca ttaaatgaat aatctttcta gcgccttgtc tggtaacata
     6001 ccccctgttg actgaagaat catgagcata aatcgctact ggcctttgat tcactttggc
     6061 taatccggta atcacctcag cgccaggaag agaatgaggt gtattatctg gaatatattc
     6121 agaacctatt ggtaaaaaac tttgttcatc aattaaatta tctatccatt taattaacgt
     6181 cttatctgta tgttgggaag acatacctct ttcctcttgc aatatttaaa tcctgggacc
     6241 agagactctc taatttagaa attagttcta tagccattat aaagcatagc actgcggaac
     6301 ttgcgtcctg aagcaaaaaa tttaaacaaa cctaccatgc tatcctatca aatttaaatt
     6361 ataaaaaatt cgcaagtatt taactaaccc atctttattt ctgaaataca cccgttgcct
     6421 ttgatttaaa catcaaagcg aaggtttaac tcaactgcat gtgttaacaa tccattgatg
     6481 tcatgctgcc ctgcataaaa tattgtttgc ccagccatgc ctggattggt gataatagtg
     6541 ctctgactgg ctttaaaatc agtggtttta aatatacgat attgcaaaat tgctgttgct
     6601 ttctcgctca ctgcgtaatt gatacctgct ttaaactgcc aagcaaaggc agttccgtat
     6661 agagatggac tattttgcat agcgggtgct gtttcgacta gaggggtatt aggatcgtta
     6721 atattaatag aattattggt ttgagtacta cttgatctta accaggcaag tcctaaacca
     6781 gcacctaaaa aaggttgtag ttttccaaca ttcattaaat cataacttgc attagtcata
     6841 aaagaataaa tgttagctct tgttgatatc ttactaataa ggttattagc ttgttgagcg
     6901 taggctgctc cagtagtact gttctgttca agccaattat aagtaccata agtgtctctc
     6961 tctatgtttt gatacaaaaa ctctaagtcc ccatgccaat ttgaactaaa atatttccct
     7021 accgccaggt taattccata accattttta aacttatttt gccaattgat gttaggtaga
     7081 gtaaataaac tagtgcctat tgcagttggt gtatacaaaa ctgagttgga attcgaagta
     7141 ttactagtac tagtattgga aaaaacggct cccaaaccag cattagcata aaaatccatg
     7201 gatggtgcta tgcccaaagt ccctgcctta gcattgtatg ctaaactcaa aaatgcaaat
     7261 gaaactagag ccgagtgata aaatttcacg aataaatcct tttaattcta atatccaact
     7321 atgaccctac tttgtatcat gaatcagtaa aaacataaac attttccact acagaaatag
     7381 tagtgtctca tgatatggag ttttaa
//
LOCUS       DAXLDT010000143         7326 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXLDT010000143 DAXLDT010000000
VERSION     DAXLDT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.143"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(273..1268)
                     /locus_tag="ACF2SP_002463"
     CDS             complement(273..1268)
                     /locus_tag="ACF2SP_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFD2360237.1"
                     /translation="MQKTARRKGIVFIIISYIILSADEPVVAKLIQLGNNFTIHGRNP
                     ISFCNLLFIGSLMGALTLFFIQYKNFRTFNFMTLTRKDWAWLFTSAFFIGFLTPSLFF
                     FGIMYANIINVILLSTLNAPITLFAAWLIFSEKPNMRLILASLITISGSFVIVLIQQW
                     TATNEKPITQITSTGPLYDFLADTPNSGEICVFLGVVSSTISTLISFHAVTTLPGGIF
                     NTFRMGLGAVFFFFIALALFGWGHFSDLLSPFLWKWMILYGPIIVGMGMFFNYLGLQH
                     IKVADDVIASSLTPLFSIVFSYLILGEAPGVAQIIGGCIIFTGIVLAMREQIRHL"
     gene            complement(1715..3382)
                     /locus_tag="ACF2SP_002464"
     CDS             complement(1715..3382)
                     /locus_tag="ACF2SP_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2552 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360238.1"
                     /translation="MKEIVSLLEEDQIDFANHKRPEVLMVLRRKFRTQKDWIITSFNQ
                     YQFTKEESQKVQQAILNGDLRITDLLNRPFSHYFWSFLTFKWGEILSGGKRLSKQEVL
                     QIASCSSNGQEFARTPTQNRNRLIKGVPLVIGKVVTSMALCMVFCLGLLSTLGAPFGL
                     SLGLAIVLSLCNGIVCLLSRGKAMLDSAGFQPQKQKTFKTLEENIKEKTIFDRGQKLF
                     FGVVTLLACISAGISGYAGLVGLLTFLGAGMLISNPLVLILTIGLSVVAAVSFYSFQA
                     IGIRENYTKFKNLFIEDYQKPYGLLRCVLLGMVSTVFLAVYATLTWFTTVSGVNKLFN
                     ALGATPDTLLAHFIAMICTSTTMVVNTFSQVYKVFNPKTYVDLKEKFESIYQPPEENL
                     TRAQKIQHGVINMLKLFALIGDSLAYSVAGGIRSGIHIGETLGETIGAKFALTITMAI
                     LSPVILVFVSIAFTWPTVFDRKKSTPCPSEMDKPLIEKMNQSHTSDALQNNTLLPTSE
                     KSPKPNDSNIRQAGLRFFDVGLSEEHTHKPNCKPTLESRAVLTSPSP"
     gene            3560..4927
                     /gene="pgtP"
                     /locus_tag="ACF2SP_002465"
     CDS             3560..4927
                     /gene="pgtP"
                     /locus_tag="ACF2SP_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter protein PgtP"
                     /protein_id="HFD2360239.1"
                     /translation="MALLSFLKPAPYLDEIQDKNVVKQQYRYWRIRTFYAMYIGYALF
                     YFTRKSFTFVMPALQSTLGMTKTELGLIASILSLSYGISKFLSGILGDKSNPRYLMAI
                     GLILTGVFNILFGLSSTWWLFAIFWGLNGWFQGWGWPGCTKLLTHWYSQSERGRWWSL
                     WNTSHNVGGALIPLIVAVCAQYFGWRSAMFVPGIICILGGIFLINRLRDTPQSLGLPA
                     IEQYHEDFSGSANHHQEKTELSVKEILWNYVLCNQYIWILSISYLFIYIVRTAINDWS
                     MLYLTEVKEYSLITAGSCVIWFEVGGFFGNLVAGWSSDKIFQGKRNPINVLFTAGVFA
                     TLLLFTLTRAYTPFLDSLFLFFFGFFIFGPQMMIGMACAELAHKKAAATATGFAGFFA
                     YCLGAVLAGAPLGAIIKNYGWDNFFLTLFICCLIPLFLMLPLWHVKVNPKYRQSEFSG
                     KSEFA"
     gene            5238..5672
                     /locus_tag="ACF2SP_002466"
     CDS             5238..5672
                     /locus_tag="ACF2SP_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFD2360240.1"
                     /translation="MNYYSILLTIKRLIIGLLPILLVACAHKQSHGPDHYVVRGKTYH
                     VMKSAKHYKAKGIASWYGSHARHQKTSTGERYNMYAMTAAHPTLPIATRVRVKNLNNG
                     RSVVVRINDRGPFLSNRLIDLSFAAAKKLGIKGIAPVEIEVV"
     gene            complement(6142..6996)
                     /locus_tag="ACF2SP_002467"
     CDS             complement(6142..6996)
                     /locus_tag="ACF2SP_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2555 family Dot/Icm T4SS effector hydrolase"
                     /protein_id="HFD2360241.1"
                     /translation="MELTMAKELVFIDWDDTLVNRENAEDFESVTDENNTIYQMSPMF
                     IGAVEALKQLQEKYHVVIVSSRGDLNRLRQLQLQSYDLESSIMPLDNFTFEDNDNRLL
                     FTRYGETNFPDKASFILYIQKMSGNKIKVFIDDDHTEIEAAQSKGIPTIHATHKKYLQ
                     NTEQFFSGLIHQVDNMELSHTFNKVAAEIRNNSRSLSWGRQIAGFFSVHLPIAQKVEN
                     LASKLQRKSANEVSEEFDQYLKKIAYDPNKKGDIAASLTKAKTAIDKHMSASIEIKEV
                     QPFLNRMV"
BASE COUNT         2304 a         1336 c         1316 g         2370 t
ORIGIN      
        1 taaataactc agttgaataa aaaaaataaa atgaaaaaat cttactatag tattttgacg
       61 tttcattttt atgatcctat taaacaatgc ccccgatacc cgaatagctt ataaccatgc
      121 atccatcacg tttctatcat aaataatact ctcgttacct atttatagag ttaggataga
      181 tgccatatag aaaagaaaag cttccaaggt tactctttgt caatttttca tgcatccctt
      241 ttggggaaaa ggtaaaaata taaacagcta tctcaaagat gccgtatttg ttctctcatg
      301 gctaatacta ttcccgtaaa aataatacaa cctccgataa tttgagcaac cccgggagct
      361 tctccaagaa ttaaataaga gaaaacaatg gagaaaaggg gtgtcaaaga ggatgctatc
      421 acgtcatctg cgactttaat atgttgcagc cccagatagt taaaaaacat ccccatacct
      481 acaatgatag gcccatataa tatcatccac ttccaaagaa atggtgataa aagatccgaa
      541 aaatgccccc aaccaaacag tgctaaagca ataaaaaaga aaaaaacagc ccctaatccc
      601 atacgaaaag tattaaaaat accaccaggt aatgttgtaa cagcatgaaa acttatcaag
      661 gtggaaattg tacttgagac aacacctaaa aatacgcaaa tttccccaga gtttggtgtg
      721 tctgcaagaa aatcataaag aggtccagtc gatgtgattt gggtaatagg tttctcgttt
      781 gtagctgtcc attgctgaat aagaacaatc acaaaactac ctgagatagt gattagcgaa
      841 gctaaaatta gccgcatatt aggtttttca gaaaaaataa gccatgcagc aaataaagtt
      901 attggagcat taagagttga gagcagaatt acattaataa tatttgcgta cataataccg
      961 aaaaaaaata gcgagggtgt taaaaaacct ataaaaaaag cgctggtaaa tagccatgcc
     1021 caatcttttc tggttaatgt catgaagttg aatgttctaa aattcttata ctggataaaa
     1081 aatagtgtta gagctcccat tagactacct ataaagagga gattacaaaa tgagatggga
     1141 ttacgtccat ggatagtgaa gttattacca agttgaatca gtttggcaac gacaggctca
     1201 tccgcactta atataatata actgattatt ataaaaacta ttcccttacg tctggccgtt
     1261 ttctgcatta ttccctgtac ttaaaacttt ccttaacgtc attgtaaaat atttacagtt
     1321 catggtcacg aacctcagga aaggggcttt ccttttgctt ttcagaaaaa gggaaaggcc
     1381 tgttcctaag tttataagtt ataataagac tggttccaaa ggcaacgacg ccagatcttg
     1441 tcttttgcct atcaaagtag ttctgggtca ttttaaagaa agcaaaaaac aagattttct
     1501 ccccctgctc cgttaaattc cataatttta gagagcttag acttacaaaa taattgatat
     1561 tttcagcaaa tcaaaaacca tcgttgaatg actctggagc aattataaga aaagaacctg
     1621 caaattagtt gtttaacaag tatcgattaa atttactctt ttccagattc aaatcaatcg
     1681 atacttcact agttgattca aaaaacctca tcatttatgg cgagggagat gtcaaaacag
     1741 ctctgctctc taaagtaggc ttacaattgg gcttatgagt atgctcttcg ctcaagccca
     1801 catcaaaaaa tcttagccca gcttgcctga tatttgaatc attaggcttg ggtgattttt
     1861 ctgatgttgg taatagagta ttattttgaa gtgcatctga agtatggctt tgattcattt
     1921 tttctatcaa tggcttgtcc atctctgaag gacatggagt cgattttttt ctatcaaaaa
     1981 cggtaggcca ggtaaaagct atcgatacaa atactaaaat gaccggagac aatattgcca
     2041 tggtaatggt taatgcaaat tttgccccta ttgtttctcc caatgtttca ccaatatgaa
     2101 tcccgcttcg gattccaccg gcaacagaat aagcgagaga atcacctatc aaagcaaata
     2161 atttgagcat gttgataact ccatgctgta tcttttgtgc tcttgttagg ttttcttccg
     2221 gtggttgata tattgattca aatttttctt ttagatcgac ataggttttc gggttaaaga
     2281 ctttatatac ttgtgaaaaa gtattgacaa ccatcgttgt tgaagtacat atcatggcaa
     2341 taaaatgtgc taaaagagta tcaggagtcg cacctaaagc attgaaaagc ttattaaccc
     2401 ctgagaccgt tgtaaaccaa gttagcgtcg cataaacagc caaaaaaacc gtacttacca
     2461 ttcctaataa aacacatcgc aaaagaccgt agggcttttg gtaatcttca ataaaaagat
     2521 ttttaaattt tgtataattt tcacgtatgc cgatagcttg aaacgaataa aaagagaccg
     2581 ctgcaaccac agacaaccca attgtaagta tcaaaaccag agggttagag attagcatgc
     2641 ctgcccccaa aaacgtgaga agtcctacga ggccagcata tccagaaatg cctgcagaaa
     2701 tgcacgccaa caaggtcacg acaccaaaaa ataatttctg gcctctatcg aatatcgttt
     2761 tttcttttat attttcttca agagtcttaa acgttttttg tttttgaggc tgaaagccgg
     2821 cactatcaag catggctttc ccgcggctta ataagcaaac tattccatta cataaggaaa
     2881 gtactatcgc caagccaagg ctaaggccaa aaggcgctcc taaagtactt aataatccca
     2941 ggcaaaatac catacacagc gccatagagg taacgacttt gccaatgacc aatggcactc
     3001 cttttatcag cctgtttcta ttttgagttg gtgttctcgc aaattcctgg ccattgctag
     3061 agcagctagc aatctgtaag acttcctgct tagacaatct ttttccaccc gaaagtatct
     3121 ctccccactt aaaggtaaga aaactccaaa aatagtgaga aaaaggacga ttaagaagat
     3181 ctgttattcg aagatccccg tttagaatgg cttgttgaac tttttgagat tcttctttag
     3241 taaattgata ttggttgaaa gaggtaataa tccaatcttt ttgagtacga aattttcttc
     3301 ttaaaaccat taacacttcg ggacgcttat gattagcaaa gtcgatttga tcttcttcta
     3361 ataaactaac tatttctttc attacaataa atcctttttt aatacaaact gcgtagctta
     3421 tttataaatt cacaagaatt ccattaaatt tttatttcaa tcatcaatag gtatgatcct
     3481 gttaaatttt cactagaaag atttgacata ctttgcgagt tacattctaa tcatgttttt
     3541 ttcatgatca tggacaatca tggcattatt gagttttcta aaacctgctc catatcttga
     3601 tgaaattcag gataaaaacg ttgttaaaca gcaatatcgt tattggcgta ttcgcacctt
     3661 ttatgcaatg tatatagggt atgcgttgtt ttattttacg cgaaaaagtt ttaccttcgt
     3721 gatgcctgca ttgcaaagca ccttggggat gactaaaact gaactggggt tgatcgccag
     3781 cattttgagt ttaagttatg gcatcagcaa atttttgagc ggaattctag gtgataaatc
     3841 taatcctcgt tatttaatgg cgatcggact gattttgaca ggtgttttta atattttgtt
     3901 tggattgtct tccacctggt ggttattcgc tattttttgg ggattaaatg gttggtttca
     3961 agggtggggc tggccaggat gtactaaatt attaacacat tggtactccc aatcggagcg
     4021 tggccgttgg tggagtcttt ggaatacatc gcacaacgtt ggtggtgcat taattccttt
     4081 gattgttgct gtgtgcgcgc aatatttcgg ctggcgttca gccatgtttg tgcctgggat
     4141 catctgcatt ttaggaggta tttttttaat caatcgttta agagatactc cacaatcgct
     4201 tggcttgccc gcgatagagc aatatcatga agatttttct ggttccgcaa atcaccacca
     4261 ggagaaaaca gaactctcag tcaaagaaat tctttggaat tatgtgttgt gcaaccaata
     4321 tatttggata ttgtccattt cttatttgtt tatttacatt gtccgaacgg ccattaatga
     4381 ttggagcatg ttgtacttaa cggaagtgaa ggagtattcc ttaatcacag caggcagttg
     4441 tgtcatatgg tttgaggttg gcggtttttt tggaaatctg gttgccggct ggtcttctga
     4501 taaaatattt caaggtaaac gcaacccaat caatgttctt tttaccgcag gagtatttgc
     4561 cactttgtta ctgtttacgt taaccagggc atatacaccg ttcctcgact ctttgtttct
     4621 ttttttcttt ggttttttta tctttggccc acaaatgatg attggtatgg cttgtgcgga
     4681 attagcacat aagaaagcag cggcgactgc aactggcttt gccggatttt ttgcctattg
     4741 tttaggcgct gttctggcag gcgctccttt gggtgcaatt atcaaaaatt acggctggga
     4801 taatttcttc ctcacattat ttatttgctg cttgattcca ttatttttga tgctgccgct
     4861 ctggcatgtg aaagtcaatc caaaatatcg tcaatcagaa ttttcaggca aatccgagtt
     4921 tgcttagtga ttgacttctc tgggtaatcc agaaaaagta ggttgggtct gccttggccc
     4981 gacctgctct tgctacctcc acatgaaagc ccctggggta tagaggtatc cttgttgtga
     5041 taagataaga ggttatacag caaaatttgt tggtacatat tcatccaaaa aatgtataat
     5101 caatctaatc aatgatgaaa gcaaatgata aaaatgtgtt gcctattgac gattttcttt
     5161 gcaaaaaata tctttgcttc tcgtgatcta tgaattacgt atatttaaaa tctgtagcgg
     5221 aagtagaaga taactttatg aattattatt cgattttgtt gaccattaaa cgactcatta
     5281 taggtttact ccccatacta ctggttgctt gtgcgcataa gcaatctcat ggtccggatc
     5341 actatgtggt cagagggaaa acatatcatg tcatgaaatc tgccaaacac tacaaagcaa
     5401 aaggaattgc ttcctggtat gggtctcacg ctcggcacca aaaaacatcg actggagagc
     5461 gctacaacat gtatgccatg acagccgctc atccaacgct cccaattgct acaagagtgc
     5521 gtgtcaaaaa cctgaataat ggccgatcgg ttgtggttcg aattaatgat agaggtcctt
     5581 ttttatcaaa caggctcatt gatttatcct ttgctgccgc taaaaaactc ggtataaaag
     5641 gcattgctcc tgttgaaatt gaagtcgttt gaaaatgcta agaagtaaag cttgctcaag
     5701 ctaacaacct gattaactta ttaaataatc taaaattaag aaatcgtcct attcattaat
     5761 gaatttcgta ctcatttaga ctttaaaata actcatagta aattaagtac ttgcctgtga
     5821 aaggctattc tctgaatttt aaacttcata aaaatgatgt agagaatcat tattcaataa
     5881 gtcaacagca taaaattaaa tcgcaactgg atgattgaaa tattgattat tacagaaatc
     5941 aatgtatttt tcctttgacc attgctattg actttagata tcgatacata tacctaaatc
     6001 tcgagatttg tttgcaggat ctcaaagtta tccaccagga ttaaatcccg aggtaagccc
     6061 cgggatgtcg attgaggaag ctaaagtgaa tgacagtagt tcctggggca taccaaggca
     6121 cctagataat ggagagaatt gttaaaccat tctattgaga aatggttgaa cttctttgat
     6181 ttcaatcgag gctgacatat gtttatctat tgctgtttta gctttagtta aacttgcggc
     6241 tatatcccct tttttatttg gatcataagc aattttctta aggtattgat caaattcttc
     6301 gctaacctcg ttagcagatt ttctttgtaa tttagatgcc aaattttcta ccttctgtgc
     6361 tataggcagg tgaactgaaa agaaaccagc aatttgcctt ccccaggaaa gtgaacgaga
     6421 attgttacgt atttcagctg ctactttatt aaaagtatga gaaagttcca tgttatctac
     6481 ttgatgaatt aatcctgaaa aaaattgctc tgtattttgc agatattttt tatgagtcgc
     6541 atgtattgtg ggtattcctt ttgactgtgc ggcttctatt tctgtatggt catcatcgat
     6601 aaatactttt atcttattac cgctcatttt ttggatatac aaaatgaaac ttgctttatc
     6661 aggaaagttt gtttcaccat accttgtaaa taataaacga ttatcattgt cttcaaatgt
     6721 gaaattgtct aatggcataa ttgaagactc tagatcataa ctttgtaatt ggagctgtct
     6781 taatctattt aagtctcctc gtgaactgac gatcacaaca tgatattttt cttgaagttg
     6841 tttcagcgct tctactgccc caataaacat aggagacatt tgataaatag tattgttttc
     6901 gtctgttacc gattcaaaat cttctgcgtt ctctctattg actaatgtat catcccaatc
     6961 aataaaaact agttctttgg ccatagttaa ctccagatta atcttaaata acttaattta
     7021 acaatcacat gatcataaag attaaatttt ttacaattat agcccggtag agcaatagaa
     7081 ataactgtca ttttttaaca gtgaaatagg aataatctaa gtgtaaaaag aataggatca
     7141 gattaatcct aggtggaaat gaaggttttt taaatttttt cattcattat tgtaggctca
     7201 accctatgac aaaacctaca attaatcaat aatgcggtat caatcttttg ctgatcaaga
     7261 tcattatcag taatttcgag tgctaaaaac taatcatttg gtagtttctt tttcacaaat
     7321 ctgtct
//
LOCUS       DAXLDT010000144         7309 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXLDT010000144 DAXLDT010000000
VERSION     DAXLDT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.144"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1966
                     /locus_tag="ACF2SP_002468"
     CDS             <1..1966
                     /locus_tag="ACF2SP_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HFD2360242.1"
                     /translation="IEQTSLLVKERFEIYLNPLNQDLIKITNAINNGIINPDDSKQFD
                     KFLFESIRYNPEIFMVYYGSTTGDFIGVDREQRGIIGLTHVINSVSPPVNVRYQLNEQ
                     GEIIGKKLLTRHYDPRVRPWYQQAIKAGKPIWTNVYTFYVFDKSDFLIPGITGASPIY
                     NKNKQIKGVIALDLTIDGLQHFIRELELTKNTMIYVINNESNIIAFRTPQNKKDIRGK
                     KLTPEWIKKLNIPLKKLDFNGFEPIIKSYTHDNQKYYLAYQPISSTNEKALWHIIIIV
                     PETDVIEPLKDLSMRYILLTILVLLIGTLLVRIVSQRISNPIIQLTEEAKEITMLNLK
                     PRPLLKTRIKEVSYMDKTLSSLRSSLASFQRYVPGSLVKKLMRSGKIAQVGGQSQTIT
                     ILFSDIKDFTSISESTSPQKLMTYLSDYFQSMTEIVIQNEGTLDKYIGDAIMALWNAP
                     SKDTNHALHACLTAKIMIERLSLLNQKNKNLGFPEFKIRIGIHTGDAVVGNVGSEDRL
                     SYTALGDSVNLASRLESINKNYHTQIIVSYATFTQVSEKFPFRLLDEVAVRGKRESIE
                     IYELITAQNLENLERHKKEFQKAFSLYQKGSWKESISAFAKLTPAYSGDPLASVYIER
                     CKVLAVNPPVNWDGIWREGKTDYSLLGKFD"
     gene            complement(1987..3015)
                     /locus_tag="ACF2SP_002469"
     CDS             complement(1987..3015)
                     /locus_tag="ACF2SP_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD2360243.1"
                     /translation="MDNATIKWGILGTSFISEVMANAIQESKTSELVAIGSRTSEIAK
                     RFSEKFAIPKYYDNYQSLILDSDIDVVYIGLPNHLHKEWIIRCAQAGKNILCEKPFVI
                     GIEEIHEVISVIERTNVFCMEALMYRYHPFIKKLQEIIQSNTIGKIKLYNATYTANIA
                     EIANPVAGGSIRNLGCYPISLVRLLANAEPIEIRGIGRMNSTNNTDSQASVLLKFEDD
                     SIAAVSTADDIEMHWQFEVYGTKGHLKVDTNPWLPGCEGNKIYLSLNTESTPREISVK
                     AEKSLYTYQIDCINEQVLKGDSRQFSKMSLLDSMGNTIVLETWLKQILPSKPSLELSR
                     LKDRLPHA"
     gene            3188..5563
                     /gene="lspD"
                     /locus_tag="ACF2SP_002470"
                     /gene_synonym="gspD"
     CDS             3188..5563
                     /gene="lspD"
                     /locus_tag="ACF2SP_002470"
                     /gene_synonym="gspD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspD family T2SS secretin variant LspD"
                     /protein_id="HFD2360244.1"
                     /translation="MRSIVIILLAFVCCSLVRANDVNISTVTKDGNTFFYLQAGAYNL
                     EKDAKIRQNELAKLVDQKVEIKNLADKHLYLVQIGPIDDYQTARALKEKLSQKTEKQF
                     NQINKNQTSSLEVSQLPKNMEQAQTAPPGSKLWNLRNADIRAVIAEVSRITGKNFVID
                     PRVQGKVSIVSSTPLSSRELYQVFLSVLQVSGYAAIPNGEIIKIIPNIDAKTQSPDLL
                     SGMKSPPRGDDMMVAVIPVHYVPSEQLVPVLRPLMPQWSSVSAYAPSNMLILSGRANN
                     IKSLAEIIKQVDSSSANGIDMVRLHHALAMDVANTLKDLVKTQPGIGSRTQITIAADD
                     RSNSILVSGSKTDRIRLRMLILKLDKDSSTGVNSNTQVVYLNYLRAEDLVPILAGIAQ
                     ANFSGNVGTTIGTITRPALDSTNPASSLANTSADGQSSSLSSTSTSSAPMNASGATAN
                     TTSASTQNEGSTKPTVQIIGEPNTNSIILNAPASIIRILKTVISQLDIKPAQLLIEAL
                     VAEVDEKDVNNLGIEWGSNQQTGNPKDFRPGFAIINSKTRIDDFQAQIYALAREQRAN
                     ILSTPSVVVLDNRQAKILIGKQVSIATTSYPNNAGGTTTASPFTTFDRVNVALHLYVR
                     PQITRGQGIQLQIDQGNDTLDPATATTDNTTTPTFNISSIVTSVHVESGDIVVLGGLI
                     QDSIGNDNNKLPILGDIPGIGRLFQRNIRNRDKRVLMVFIKPIILRNERDNLHVTGEK
                     YNYVRQYQLDWIRSQEAFEQTDDETVLPPLTQGNLPVPFSTPPQYSSKMTK"
     gene            5567..7051
                     /gene="lspE"
                     /locus_tag="ACF2SP_002471"
                     /gene_synonym="gspE"
     CDS             5567..7051
                     /gene="lspE"
                     /locus_tag="ACF2SP_002471"
                     /gene_synonym="gspE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE family T2SS ATPase variant LspE"
                     /protein_id="HFD2360245.1"
                     /translation="MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFA
                     EIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSE
                     DLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
                     IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILD
                     KQAAQLDLNLLGMPEHTLKTVRQMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTR
                     NILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIA
                     VQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKT
                     PHELRQEEKELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIH
                     RNENIQTMENYLRPATPSIREDGFKRVLCGDTSLAEILRVTSQN"
BASE COUNT         2444 a         1447 c         1304 g         2114 t
ORIGIN      
        1 aattgaacaa acatcattac tcgtcaaaga gaggtttgaa atctatttaa atcctttaaa
       61 tcaagattta attaaaataa caaatgccat aaataatggc atcattaacc ctgatgattc
      121 aaagcaattt gataaatttc tttttgaatc aattcgatat aatcctgaaa tatttatggt
      181 ttactatggt tcaacaactg gtgattttat aggagtagat agagagcaaa ggggaataat
      241 aggattaacc catgtgatta attccgtatc acctccagtc aacgttcgtt atcaattaaa
      301 cgaacaagga gaaataatcg gaaaaaaatt gctgacccgc cattatgatc cgagagttcg
      361 cccgtggtat caacaagcta taaaagcagg taaaccgata tggacaaacg tatatacatt
      421 ttatgtattt gataaaagtg acttccttat ccctggaatt accggggcct caccaatata
      481 taataaaaat aagcaaataa aaggagtgat tgctcttgac ctgactattg atggtttaca
      541 acattttatc cgagaactgg aattaactaa aaacactatg atttatgtga ttaataatga
      601 gtccaatatc attgctttca gaaccccaca aaataaaaaa gacatccgtg gaaaaaaatt
      661 aaccccggaa tggataaaaa agctgaatat tccacttaaa aaactggatt tcaatggttt
      721 tgaacccatc atcaaatcgt atactcatga taatcaaaaa tattatttag cataccaacc
      781 tatatccagt acaaatgaaa aagcactgtg gcatattatt attattgttc ctgaaacgga
      841 tgtgatagaa ccactgaaag acttaagcat gcgctatatt ttgttaacta tactggtttt
      901 gctgattggg acattattag ttcgtatcgt ttcacaaaga atatcaaatc ctattattca
      961 actgactgaa gaagctaaag aaattaccat gcttaacctc aaacccaggc cacttctgaa
     1021 gaccaggatt aaagaagtca gttatatgga taaaacacta tcatctctgc gatcaagctt
     1081 agcttcattt caacgctatg ttcccggatc tttggtaaaa aaattaatgc gtagcggaaa
     1141 aattgctcaa gttggaggac aaagccaaac aattactatt ttattttcag atataaaaga
     1201 ttttacaagt atttcagaat ccacctcacc acaaaaatta atgacctatt tatccgatta
     1261 tttccagtcc atgaccgaga tagtaataca aaatgaaggg actttggata aatacattgg
     1321 cgatgctatc atggcattat ggaatgcacc ttcaaaagat accaatcatg cattgcatgc
     1381 atgcctgact gccaaaatca tgattgagcg ccttagcctg ttaaatcaaa agaataaaaa
     1441 tcttggattt cccgaattta aaatcagaat tggcattcac accggagatg ctgttgtggg
     1501 taatgttggt tcagaggaca ggctaagtta tacagccttg ggcgactcag tcaatctggc
     1561 aagtcgcctg gaatcaatta ataaaaatta tcatactcaa atcattgtca gttacgccac
     1621 tttcactcaa gtatctgaaa aatttccttt ccgtttgtta gatgaagtag ctgtaagggg
     1681 taagcgtgaa agtattgaga tatatgaact gattacagct caaaatcttg aaaatcttga
     1741 gcgacataaa aaggaatttc aaaaagcatt ttctctctat caaaaaggtt cctggaaaga
     1801 gagtataagt gcttttgcaa aacttactcc agcctactct ggtgatccat tagcttccgt
     1861 ttacatcgaa cgctgcaagg tattagcagt taatccacct gtcaattggg atggtatctg
     1921 gcgcgaagga aaaacagatt actctcttct cggaaaattt gactagcatc accagaacca
     1981 ttttgcttat gcatgaggca gcctgtcttt taatctggat aattcaaggc ttggttttga
     2041 agggagtatt tgttttagcc aggtttccag aacgattgta tttcccatgc tatctaataa
     2101 agacatcttg ctaaattgtc tggaatcccc tttcaatact tgctcattga tacaatctat
     2161 ttgataggta taaagtgact tttctgcttt cacgcttatt tctctgggtg ttgattcagt
     2221 attgagagag agataaattt tgttaccctc acagccaggc aaccaaggat tcgtgtctac
     2281 ttttaaatgg ccttttgttc cataaacctc gaattgccaa tgcatttcaa tatcatcggc
     2341 agtggaaact gctgcaattg aatcgtcttc aaattttaaa agaacgctgg cttgactatc
     2401 cgtgttattc gtggaattca ttcttccaat accacggatt tcgattggct ctgcgttagc
     2461 aagcagcctc actaaggaaa ttgggtaaca tccgagatta cgtatacttc ctcccgcaac
     2521 aggattggct atttctgcaa tgtttgctgt gtatgtcgca ttatacaact ttatcttacc
     2581 tattgtgttg ctttgaatga tctcttgtaa tttttttata aagggatgat atcggtacat
     2641 tagagcttcc atacaaaaaa catttgttct ctcaattact gaaataacct catggatttc
     2701 ttctatacca ataacaaaag gtttttcaca caaaatattt tttcctgcct gcgcacaacg
     2761 aataatccat tctttatgca gatgattagg caaccctata taaaccacat ctatatcact
     2821 atcgagaatt aatgattgat aattgtcata atattttgga atagcgaatt tttcagaaaa
     2881 tcgtttggct atctcgcttg ttcgactccc aatagcaaca agctcactgg tttttgattc
     2941 ctgaattgca ttagccatca cttcagagat aaaacttgtt cctaaaattc cccattttat
     3001 tgtcgcatta tccatatatt ccttccggct aaaaaattgc tgactgtata ttaaaacgca
     3061 taaatgacac gagtaaaacc atagcagtaa aaaccactta aatttcctgc tccaagttat
     3121 caaaaaagct atggtaagca gtcaaattta gttattatgt ctttatccat tattttacgg
     3181 tttttaaatg cgctcgatag taattatact tttagccttc gtttgttgtt cattagtacg
     3241 tgctaatgat gtcaatattt caaccgtaac caaagatggc aatacatttt tttatcttca
     3301 agctggcgcc tataatctcg aaaaagacgc taaaatacgc caaaatgaac ttgccaaatt
     3361 agtcgatcaa aaagttgaaa ttaaaaacct ggctgacaaa catctttacc ttgtgcagat
     3421 tggtccaatt gatgattacc aaactgcaag agcactcaaa gaaaagttat cgcaaaaaac
     3481 agaaaaacag tttaatcaaa ttaataaaaa ccaaacctct tcacttgagg taagtcaatt
     3541 gcctaaaaat atggaacaag cgcaaacggc tcctccaggc agtaaattat ggaatttacg
     3601 taacgctgat attagagccg taatcgcaga agtctctcga attacaggta aaaattttgt
     3661 tattgacccc agagtacaag gaaaagtatc gattgtttca tcaacgccgt tatccagccg
     3721 cgagctatat caagtctttc tctcagtact gcaggtatca ggttatgccg caatacctaa
     3781 cggagaaatt atcaaaatta ttcccaatat tgatgcaaaa acacagtcac ctgatttact
     3841 cagtggaatg aagagtcctc cacgaggtga cgatatgatg gtcgctgtaa tacccgttca
     3901 ttatgttcct tctgaacagc tggtacctgt tctaaggcca ctaatgcccc aatggagcag
     3961 cgtatctgcc tatgctcctt ctaatatgtt gatcctgtct ggtcgagcaa acaatattaa
     4021 aagccttgcg gaaattatca aacaagtaga tagctcaagt gcaaacggca tagacatggt
     4081 tcgtttacat catgctttgg ctatggatgt tgcaaacact ttaaaagacc tggttaaaac
     4141 tcaacctggt atcggcagca gaacacaaat taccattgct gcagatgacc gttccaactc
     4201 aattttagtc agtgggagca agacggatag aattcgtttg cgaatgctga tattgaaact
     4261 ggataaagac agctcaacag gagtcaattc aaatacacaa gttgtttacc tgaattattt
     4321 aagagctgaa gatttagtcc ctatactggc aggtatagcc caagccaatt tcagtggaaa
     4381 tgtaggaaca accattggaa ccattacaag acccgcatta gacagcacca accctgcttc
     4441 cagtctcgcc aatacatctg ccgatgggca aagctcttca ttaagctcca cttcgaccag
     4501 ttctgcccct atgaatgcgt caggagcaac agcaaatacg acatcagcaa gtactcaaaa
     4561 tgaaggctct accaagccta ctgtacagat aataggagaa ccgaacacta actctattat
     4621 tcttaatgca cctgcttcaa ttattcgcat tctaaaaaca gtcatttctc agctagatat
     4681 aaaaccagct caactattaa ttgaggccct cgttgctgaa gtggatgaaa aggacgttaa
     4741 caatctggga atagagtggg gttcaaatca acaaacaggc aatcccaagg atttcagacc
     4801 aggctttgcc attataaaca gtaaaaccag gattgacgat ttccaagctc aaatttacgc
     4861 cttggcaaga gagcaaagag caaatatttt atcaactccc tccgtagtag tccttgataa
     4921 tcgtcaagca aaaatactca taggtaaaca ggtctcaata gcaacaacca gctaccccaa
     4981 taatgcagga ggcactacca cagcgagtcc tttcacaaca tttgacaggg ttaacgttgc
     5041 tttgcacctt tatgtaaggc cgcaaattac tcgcggacaa ggaattcaac tacagattga
     5101 tcaaggaaat gatactttgg atccagcgac tgctacaaca gacaacacaa caacccccac
     5161 ttttaatata agcagtattg taacttcagt tcatgtagaa agtggtgata ttgtagtact
     5221 tggagggttg attcaggaca gtattggaaa tgataataat aaattaccca ttttaggaga
     5281 tatccctgga ataggcagat tgtttcaacg taacattcgc aacagagaca agcgagtgct
     5341 tatggtattt attaaaccta tcatattacg caacgaaaga gataacttgc atgtcacagg
     5401 cgaaaaatac aattatgtcc gtcaatatca gctggattgg attcgctccc aagaggcttt
     5461 tgaacaaaca gatgatgaaa cagtgttacc cccattaact cagggcaatc tcccagtacc
     5521 ttttagtact cctccccaat actcatcaaa aatgaccaaa taaatcatgg aaaatgaaga
     5581 aaaatcactt aaaattcctt acagttttgc caaaagcaat ggaattgttg tgggagaaat
     5641 acaagaaaat ctggctattg tctatcactt acctaacaca ccactgcaag ctttcgctga
     5701 gataaagcga ttattacaat gtgaacttga tctaaaacaa gtcgatgaat ccacttttca
     5761 gcaacattta gccaatattt accaatctaa atcttcaata ctggatgctg cagaaggcat
     5821 ggaagaagat atggatttat ccatgttggc cagtcaactt cccgtcagtg aagacttatt
     5881 ggaaaaccaa gacgatgccc caattatccg cttactgaac gctctgttta cccaagcgat
     5941 taaacaaaaa gcatctgata tacacattga aacttatgaa gacagagtat tggtgcgcaa
     6001 tcgaattgat ggtgttctac aagaggtcct tgaaattcaa cgagctatcg ctcctttggt
     6061 catttccaga gttaaggtga tggccaaatt ggatatcgcc gaaaagagaa ttcctcaaga
     6121 tggtcgaatt tcattgcgta taggaggaca taatattgat gtcagggtct caactttacc
     6181 ctcaaaccat ggagaacgag tggttttaag gatattggat aaacaagctg ctcaactgga
     6241 tttaaatctt ttgggtatgc ctgaacatac attaaaaaca gtacgacaaa tgattgctga
     6301 accccatggc attattttag tcactggccc aacaggttca ggcaagacaa catctcttta
     6361 tgctatgctc accgaactaa atcaggtgac tcgtaatatt ttaaccattg aagatcccat
     6421 tgaatatgac ctgcctggtg taggacaaac gcaggttaat actaaagttc aaatgacttt
     6481 tgccaaagga ttaagggcca tattacgtca agatccggat gttgtcatga taggagaaat
     6541 ccgtgatttg gaaacggctg aaattgctgt tcaagccagc ttaacagggc atttggtact
     6601 atcaaccctt cataccaata ccgcattagg agcattaact cgcctgcatg atatgggagt
     6661 tgagtctttt ttattgtcat ccagtatagt cggcctcatt gcgcaacgtt tggtaaggaa
     6721 attatgctct cattgcaaaa cacctcatga attaaggcag gaagaaaagg agttaatggg
     6781 tcttaaacct gatgctgatg tatctcaggt ctttgagccc aagggatgcg actattgcaa
     6841 ccacttgggt tataaaggca gaacaggaat ttatgaatta attatcgtag atgaaaccct
     6901 gagaggaatg attcatcgta atgaaaatat tcaaacaatg gaaaattatt tacgacccgc
     6961 cacgccgagc atacgtgaag atggttttaa aagagtatta tgtggcgata cgtcacttgc
     7021 agaaatttta agggtaacat cccaaaatta attcttttcc cagtacaaag actaatgtta
     7081 agtcctacta tacacgttat ttaacttctg aattctatac tgacttaaac ttccaaaaaa
     7141 tcataatgat ctcaagtgcg cttaaattta gaataaatag gtatataatc aaaatgttgc
     7201 acagaatttg cagaaccaca ttcatcatat gtattatctg cacaaagaaa aaactctttt
     7261 gcccctaatt cggtagcacg agacaccgct tcatcaatcg aattaaaat
//
LOCUS       DAXLDT010000145         7289 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXLDT010000145 DAXLDT010000000
VERSION     DAXLDT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.145"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            15..1562
                     /locus_tag="ACF2SP_002472"
     CDS             15..1562
                     /locus_tag="ACF2SP_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HFD2360246.1"
                     /translation="MANTIDFYPITPLAKAHWKRISINKIKARTLGKIFVFIMAITIM
                     LTLFNFSYATDSKLKPTVITVSEEEFQVDGKGKKTKEFKLTFTHPDGTKDNKGYFANK
                     RDMFHVLVENKSASPITLHWHGLIVPSDQDGVPGVSQILIPPGQNKLFNYRLLQAGTY
                     WMHSHQKFQEQKQLSAPLIIYDKDDPHKDLQEVILFLEDFTYKDPQLIFDQLRTAKMD
                     MKKMDSGNDLNDVTYDAFLANKKTLAAPDVISVQPSKKVRLRIINASSSTNFKIDTGK
                     LSATLIAVDGENIEPIVDSSFPIGVGNRMDLLVEIPSAGGAFPVKAMAEGTNKQTGLV
                     LTTDNTPTPILSPNTTNKTGRVNYYELEKKLKGKNALTNKKINARLHYVLDGQMNGYI
                     WTMNKQSWPDVTPKVIHFGDRVEMIYENKSSMSHPMHFHGHVFQVTEIDGQPLDGAMR
                     DTLLVQPHSTVKVQFDALNPGIWANHCHNLYHLNAGMFTTFEYQHYPKPDFYLKTIGQ
                     IKKSKAN"
     gene            complement(1667..2116)
                     /locus_tag="ACF2SP_002473"
     CDS             complement(1667..2116)
                     /locus_tag="ACF2SP_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360247.1"
                     /translation="MRKNLLKTMIVLSIGLLTYETTFSLPQIILNKADDFSIEIGTVS
                     KSTFSSCGNSIANCLVTLKLMIHNEGFVIIHNNSSRTLTNVRAYFPEELQNQVDQNAS
                     ACISLAPGLSCRLTFRPTTMSQFLERTAIPIHGDKTSTAFFDLQITE"
     gene            2277..2528
                     /locus_tag="ACF2SP_002474"
     CDS             2277..2528
                     /locus_tag="ACF2SP_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360248.1"
                     /translation="MLRLLMSGWTCWFILFLSLGLSSCASEPTTVQQAVQNAVSRLTP
                     EQLATYNATSDQFNQWNASSQFNISTGELSFLIVGCGNT"
     gene            2575..3555
                     /locus_tag="ACF2SP_002475"
     CDS             2575..3555
                     /locus_tag="ACF2SP_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360249.1"
                     /translation="MKPCIFLSFLLFFASTCFSKTESIETQGALKNTQEIEQLLRHYL
                     KPKAVLNSSYDEFKFASYQGALFNLYSGHTNFNGIGAENLRLGNFYWGLNLYNITTNT
                     DSNSRITNALNQNHGSIEDNGIYFHVLKQITSLVFLDVFANFGRDRFRLINTVTIDGN
                     TPLTGYAKYYGNDNTVGVRTFLGQTYKLFYLQGDLTFLYSNFNQPDYILTFPDQPVGV
                     PALTTKIGTLVEHARLYYQVNEHFSPFLNGGLIQLTSRSFSRPVLGPDFVTIAPLPQL
                     LLAKNGYSYGIGFDYLYKLIRITPIYAHTVRGNSFSDDYVGVKLELKGTS"
     gene            complement(3692..4348)
                     /locus_tag="ACF2SP_002476"
     CDS             complement(3692..4348)
                     /locus_tag="ACF2SP_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFD2360250.1"
                     /translation="MKIIISILFAFISASGHAFSCYDPQTIIQKPIQFDKKRIALTRE
                     YQLTHYGIDSESIEIEPKMIVLHWTCIPSLDTTFRIFDPPALPTSSPRRNELPGDLNV
                     SSHFLVDRDGTIYQLMPETWMARHVIGLNHYAIGIENIGGVDSKDDLTEEQAKANAFL
                     VCYLKNKYPQIKYLIGHNEYLQFKGTALWLEKDPNYQTDKTDPGPTFVKKVKQLIAAS
                     "
     gene            complement(4469..5743)
                     /locus_tag="ACF2SP_002477"
     CDS             complement(4469..5743)
                     /locus_tag="ACF2SP_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360251.1"
                     /translation="MNSQKDLKQLDIKQVIAWAFYDFANSSYFVVIFTFVFATYFTSY
                     IAPNTILGTELWGYTISVSALLIALVSPIVGAIADFSGHHKSWLFVFTYLGVISTGCL
                     WFAYPDSHSIPLVLTCILISNFALEVGTVFYNSFLANLAPENYLGRISGWAWGCGYLG
                     GLLCLILSLFVFVKGELGGLWFGTENFANIRAVTVFVALWISLFSLPLFLMVQQKTGE
                     HLTVGTAIRKGLQELRLTIKSLPKQRDLFLFLIARIFYIDGLNSILALGGIYAAGNFH
                     LSVSDIMVFGIILNITAGLGAGLFAWFDDWIGSKKTILIALACLTLTFCFLLTIHSNA
                     LFWVFAPVFGIFVGPVQAASRTFLARLAKPEEMTRMYGFYSFSGKATSFLGPFLVSTT
                     TALADSQRLGMAVLIPFFIIGGGLLLLVHENE"
     gene            6002..6640
                     /locus_tag="ACF2SP_002478"
     CDS             6002..6640
                     /locus_tag="ACF2SP_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFD2360252.1"
                     /translation="MPDVLNNYYLQTMGIEVWVMRESTPSCEKHLATLADEVASCVRC
                     SLHKTRTQTVFSRGNPKAKLMIIGEAPGFYEDQKGQPFVGKAGFLLNKMIRSIEMTEN
                     DVYIANVLKCRPPNNRDPALEEISQCSSFLTRQIKLIKPHLILALGRFAGQFLLDKPL
                     PLKQLRNHIHYYNNTPFIVSYHPAYLLRNPSDKKKAYIDWLAVKSFLVDYGQ"
     gene            complement(6726..7124)
                     /locus_tag="ACF2SP_002479"
     CDS             complement(6726..7124)
                     /locus_tag="ACF2SP_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0260 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360253.1"
                     /translation="MFGFFSDYKQYITLRNFAVVYNGLTGLAVLYSLWSNPEADPSEY
                     VIDISIHALTAITLMCKQAPESVKAVAMALNTYRGFDALFKAITSLPSTIPGIANAVD
                     VLNHRFNFKELEKLGNEETAETRSAVQHTM"
BASE COUNT         2400 a         1466 c         1302 g         2121 t
ORIGIN      
        1 gaaagatcag cgaaatggcc aatactatcg atttttatcc aattacccct ctcgccaaag
       61 cccattggaa aagaatatca atcaataaaa ttaaagcacg tactttgggt aaaatattcg
      121 tttttatcat ggcaataact atcatgttaa ccctttttaa tttctcttat gcaacagata
      181 gcaaacttaa accaacggtc ataaccgtca gtgaagagga attccaggtc gatggtaaag
      241 gaaaaaaaac aaaggagttt aaactcacct ttacccaccc ggacggcaca aaggacaaca
      301 aaggttactt tgccaataaa agagatatgt ttcatgtttt agttgaaaat aaaagtgcgt
      361 caccaataac tcttcattgg catggattaa ttgtcccaag cgatcaggat ggagtacctg
      421 gcgtgagtca aatcctgatt ccaccagggc aaaataaatt atttaactac agattattgc
      481 aagccggaac ctattggatg cactctcatc aaaaatttca agagcaaaag caactttctg
      541 cccctttgat tatttatgac aaggatgacc cgcataaaga cttacaagaa gtcatcctgt
      601 ttttagagga ttttacttat aaggatcccc agttaatatt tgatcagtta agaaccgcta
      661 aaatggacat gaagaaaatg gattctggaa acgatttgaa tgacgtgacc tatgatgcat
      721 ttctcgccaa caaaaaaaca ttggcagcac ctgatgtgat ttcagttcaa ccctctaaaa
      781 aagtaaggtt acgaatcatc aatgcgtctt cctccaccaa ttttaaaatt gataccggga
      841 aactatcagc cactttgatt gctgttgatg gtgaaaacat tgagccgatt gttgatagtt
      901 ctttccctat tggcgttggt aatcgtatgg atttgcttgt tgagattcct tctgcaggcg
      961 gggcattccc tgttaaagcc atggcggaag gaacaaacaa acaaaccggt ttggttctaa
     1021 caacagataa cacacctacc ccgatactat ctcccaatac cacgaataaa acgggacgtg
     1081 tcaactacta tgagttggaa aaaaaactaa aaggaaaaaa tgctttaact aataaaaaaa
     1141 taaatgctcg tttgcactat gtgcttgatg gccagatgaa tggttatatt tggaccatga
     1201 acaagcaaag ctggccagat gtcacgccaa aagtaatcca tttcggtgat cgtgttgaaa
     1261 tgatctatga aaataaatcc tctatgtcac accccatgca tttccatggt catgtctttc
     1321 aagtaaccga aattgatggt cagccattgg atggagccat gcgcgatacc ctgttagtcc
     1381 aaccgcattc gacagtgaaa gtacagtttg atgcgctaaa ccctggcatc tgggctaacc
     1441 actgccataa tttatatcat ttaaatgcgg gaatgtttac aacgtttgaa taccaacact
     1501 acccaaagcc cgatttttat ctaaaaacaa tagggcaaat taaaaaatca aaggctaatt
     1561 agtaatttat ctttggctta gccatggttc agaagctctg ttcactggct tttcgttgct
     1621 acaccaatat tgcccctaca gtctgatcag aaaaatatat gtgtctttat tccgttattt
     1681 gtaaatcaaa aaatgccgta cttgtcttgt ccccatgaat tgggatagct gttcgctcta
     1741 aaaactgact catagtagtt ggtctaaaag ttaaccgaca ggataagccc ggagccaatg
     1801 aaatacatgc agaggcattt tgatcaacct gattttgtaa ttcttccgga aagtaagccc
     1861 tgacattcgt cagcgttcta ctcgaattat tatgaataat aacaaaaccc tcattatgaa
     1921 tcatgagctt tagggtaacc agacagtttg ctatggaatt cccacaagag ctaaaagtgg
     1981 atttagatac agtgccaatc tcaatcgaga aatcatcagc cttgttcaag attatttgag
     2041 gtaatgaaaa agtagtttca taagttaata atccaatact gagaacaatc attgttttca
     2101 ataagttctt acgcataaat cgatcctttg ctttttgtta atctgataat tgaacttaaa
     2161 aattcaaaaa accatatata ttctagcccc atttataata cactgagtca cttacaaatt
     2221 cttattttat ttcgaacacc cttaaatatt tattttttaa ttacaggagg tatacagtgt
     2281 tgcgtttact gatgtctggg tggacttgtt ggttcatttt atttctatcc ttaggacttt
     2341 ctagttgtgc cagtgagcca accaccgttc aacaggcagt tcagaatgcc gttagccgct
     2401 taacccccga gcaactcgca acttataatg caacctctga tcaatttaat caatggaacg
     2461 ccagcagtca gtttaatatt tccacaggtg agctttcttt tttaattgtt ggctgcggaa
     2521 atacatagaa agaattttat aaacaaataa ttaactattt tttaaaggaa tataatgaag
     2581 ccctgtattt ttttgtcatt tcttctattc tttgcatcca cctgtttttc taaaactgaa
     2641 tccattgaaa cccaaggtgc tttaaaaaat acgcaagaaa tagagcaact attacgccat
     2701 tatttaaaac caaaagccgt tttgaatagt tcctatgacg aatttaaatt cgcatcatat
     2761 caaggagcat tatttaatct atattccgga cataccaatt tcaatggtat aggagcggag
     2821 aatctgcgcc tgggcaattt ctattggggg ctgaatcttt acaacataac aaccaatact
     2881 gattcaaact caagaataac caatgcactg aaccaaaatc atggatctat agaggataat
     2941 gggatttatt ttcatgtatt gaaacaaata acctcgttag tttttcttga tgtattcgcc
     3001 aattttggac gagatcgctt taggttaatc aatacggtca caattgacgg caatacccca
     3061 ttaaccggtt atgcaaaata ctacggaaat gataatacag ttggagtacg cacctttttg
     3121 gggcaaactt ataaattgtt ttatttacaa ggggacttga cctttttata cagtaatttc
     3181 aatcaaccag attacattct gacattccca gatcaacctg tgggtgtgcc cgcactgaca
     3241 accaaaatcg gaacgctggt tgagcatgcc aggctttatt atcaagtcaa tgaacatttt
     3301 tccccctttt taaatggcgg tttaattcag ttgactagcc gatcttttag cagacctgta
     3361 cttggtcctg actttgttac catagcccca cttcctcaat tgctattggc aaaaaatggt
     3421 tacagctatg gtattggttt tgattatttg tataaattaa tacggattac cccgatttat
     3481 gcacacacag tccgtggtaa tagttttagt gatgattatg tgggagtaaa actggagcta
     3541 aaagggacga gctaattcgt aatttgataa ctcctaaaaa tcaaatttca taagcatact
     3601 gtatagatgc atcactctta ttattaataa gtagtgtaac ttgaattaaa tacttgagat
     3661 aaggaaaata tgttttaatg agtttatcag tttatgacgc agcaattaat tgttttactt
     3721 ttttaacgaa cgttgggcca ggatctgttt tatcggtttg ataatttgga tccttctcca
     3781 accacaaagc cgttcccttg aactgcaaat actcattatg accaataagg tattttattt
     3841 gcgggtattt gttcttgaga tagcacacaa gaaaggcatt tgccttggct tgctcctcgg
     3901 tcaaatcgtc tttactatca acaccgccaa tattctcaat accaatagcg taatgattca
     3961 agccaatcac atgccttgcc atccatgttt ctggcatcag ttgataaata gttccatccc
     4021 gatcgactaa aaaatggctt gatacattta aatccccagg caattcattt cgtcttggcg
     4081 aactggtggg taaagcgggg ggatcaaata tgcggaaagt agtatcgagg cttggtatac
     4141 aagtccaatg caatacaatc attttaggct caatttctat cgactcggaa tcaatgccat
     4201 aatgagtaag ctgatattcc cgggttaagg caatccgttt tttatcaaac tgtatgggtt
     4261 tttgaatgat agtctgtgga tcatagcaag aaaacgcatg cccagaggcg ctaatgaaag
     4321 caaataatat agatataata attttcatat tagaacaaag tttatcataa gaaataactt
     4381 gtaaaaacaa agaggcgaat ttttcccctg tctttcaaac tcatactgaa cttcaaaaca
     4441 gggaaaatca gccttaccgg aatttttctt attcattttc atgaaccaat aataatagtc
     4501 cgcctccgat gataaaaaaa ggaataagaa cagccatccc caaacgctga ctatcagcta
     4561 aagcagtggt tgtactaacc aggaaaggtc ctaaaaaact ggttgcctta ccagaaaaag
     4621 agtaaaaacc atacattcgc gtcatttctt caggttttgc caaacgagcc aagaaagtac
     4681 gactggcagc ctgaacagga ccaacaaaaa taccaaacac cggtgcaaaa acccaaaata
     4741 acgcatttga gtggatggtt aacagaaaac aaaaagtgag tgtcaggcac gctaaagcaa
     4801 ttagaatagt ttttttagat ccaatccaat catcaaacca ggcgaataat ccggcgccaa
     4861 gacctgcagt aatatttaaa atgattccaa acaccataat atcgctaaca ctcaagtgaa
     4921 agttgccagc cgcataaata ccgcctagtg ccaatataga attcaaacca tcgatataaa
     4981 aaattctggc tattaaaaat aagaatagat cacgttgttt aggcaggctt tttattgtta
     5041 accgcaattc ttgcagacct ttcctaatcg cagtacccac tgtcaaatgc tctcctgttt
     5101 tctgttgcac cattaaaaac aaaggcaaac tgaacaaact aatccacaaa gccacaaata
     5161 cagtaacagc acgaatattg gcaaaattct ctgtcccaaa ccataatcca ccaagttcac
     5221 ctttcacaaa aacaaataag gatagaatca ggcaaagcaa tcctcccaga taaccacaac
     5281 cccaagccca gccagaaatc cgccccagat agttttccgg ggctaaattt gccaaaaagg
     5341 aattataaaa tactgtccct acttccaatg caaaattgct aatcaaaata caagttaaaa
     5401 ccaaaggaat agaatggctg tcaggatagg caaaccacag gcaacctgtt gaaataacac
     5461 ccaaataggt aaaaacgaat agccaggatt tatgatgccc actgaaatca gctattgcac
     5521 caacgatagg gctcaccagg gcaatgagta acgcagaaac cgaaatcgta tacccccaca
     5581 attcggttcc caggatagta ttgggggcaa tgtagcttgt gaaataggtt gcaaagacaa
     5641 acgtaaatat cactacaaaa taggaagaat tggcaaaatc gtagaatgcc caggcaatca
     5701 cttgcttaat atctaattgt ttcaaatcct tttggctatt catattcttt ccagtctctg
     5761 ttcaatattc ttcaccccct caactctcta tttcttacag gcagtgagta tatgatgttt
     5821 ttttaagttt ctttctttaa aataggactg atacaataga ttaactatag gacttaagca
     5881 aaaaactcca aagccaagac aaataatgtc tttaacaaag agatcggagg tgcgtaagta
     5941 ccaaactaca aaagataagt ctaatctata atccggattt tattaatatc atgagttttt
     6001 tatgccagat gtattgaaca attactacct gcagactatg gggattgaag tctgggtaat
     6061 gcgcgaatca actccttctt gtgaaaagca tttagcgact ttagcggatg aggtggcatc
     6121 ctgtgtacgt tgttccctcc ataaaacacg cacccaaact gttttttctc gcgggaaccc
     6181 aaaagcaaaa ctgatgatta tcggtgaagc gcctggtttt tacgaggatc aaaaagggca
     6241 accttttgtg gggaaggctg gttttttatt aaacaagatg atacgtagta ttgaaatgac
     6301 agagaatgac gtctacattg ccaatgtttt gaaatgccgt ccgcccaata accgtgatcc
     6361 agctcttgag gaaatttctc aatgcagttc attcctgacc cgccaaatta aactcattaa
     6421 accgcactta atccttgctt taggacgttt cgccggacaa ttcttgcttg ataaaccact
     6481 tcctctgaag cagctgcgta atcatattca ctactataac aatacccctt ttatcgtaag
     6541 ctatcaccct gcctatcttt tgcgcaaccc ctcggataaa aagaaagctt atattgattg
     6601 gttagctgtg aaaagttttc tggtggatta cggccaataa actgtttagc atgaaattgg
     6661 cgtaagagaa cccgatagaa aatggctgat aactccatct tctatcggaa aagacgatcc
     6721 agactttaca ttgtatgctg aactgctgat ctggtttctg cagtttcttc attgccgagt
     6781 ttttcaagtt ccttaaaatt aaaccggtga tttaatacat caacagcatt ggcaatgcct
     6841 ggtattgtgg aaggaagtga ggtgatggct ttgaataaag catcaaatcc cctgtacgta
     6901 tttaatgcca tggcaactgc tttaaccgat tcgggtgctt gtttacacat taaagtgata
     6961 gcagtcagcg cgtgaataga gatatcaatg acgtattcag agggatcagc ctcaggatta
     7021 ctccataagc tatacaatac tgccaagcct gtaagcccgt tataaacgac agcaaaattt
     7081 ctcagagtga tgtattgttt gtaatcacta aaaaaaccaa acatagatta ttcctttcaa
     7141 aataaaacgt atctaatgtg acttaatagt cttcgtacca ttttggccgc aaaagcatag
     7201 cactatttta tagctgatga aaataatttg attttaaagt actctgtacg gtacgatttt
     7261 tgggaccgtc gttgcgagga gcgtagcga
//
LOCUS       DAXLDT010000146         7224 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXLDT010000146 DAXLDT010000000
VERSION     DAXLDT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.146"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..765)
                     /locus_tag="ACF2SP_002480"
     CDS             complement(<1..765)
                     /locus_tag="ACF2SP_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HFD2360254.1"
                     /translation="MIRSILVLFFLFIANAGYAWNAIGHQLVAQIAYDNLTPQSRRMC
                     DLYSHSKSKTSSNVNFVKSASWLDSIRAHDVHWFDALHYIDIPFSMDETELPALPDIN
                     ALWGINQAIAVLSSNKASIADKKLSLRILVHLVGDIHQPLHTVTKISKKLPKGDLGGN
                     LFQLAKNPIGNNLHQYWDNGGGILIGQDKFFQIKNKARQLEKKWSCQSASKEKNPQQW
                     INASHQLALTKVYKVSAHQVPGKQYQLNTQNITEKQI"
     gene            complement(768..2027)
                     /gene="tolB"
                     /locus_tag="ACF2SP_002481"
     CDS             complement(768..2027)
                     /gene="tolB"
                     /locus_tag="ACF2SP_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFD2360255.1"
                     /translation="MCNRIISLFLLLFTGQVIALDLELTQGINSALPIAINSFGSDAA
                     AQEIGNVIENDLTISGQFKIISGPQGANSQSSVSTLRQLGADSVVTGRVNQVGNRIEV
                     SFTLADAVANGNILLTKTFQINANQVRALAHHISDEVYQKLTGERGIFSTRIAYISVQ
                     RNGGRSRYSLEVADADGHNPQSLLVSSEPIMSPSWSPNGKSISYVSFEKKKAEIFTVS
                     VETGQRRLITSFPGINGAPAWSPDGRHLAVVLSKSGTPKIYDVDLSSGSMKQLTFGNS
                     IDTEPRYSPDGRSLLFTSGRGGSPQVYRLSLADGQISRVTFEGNYNARASYTPDMKHI
                     VMLHREDRQFNIGVQNTGGGPISNLTFSGLDESPSVSPNSRLVLYATRYQDRGVLGIV
                     SIDGRIRMRLPAREGDVQEPAWSPYLS"
     gene            complement(2033..3031)
                     /gene="tolA"
                     /locus_tag="ACF2SP_002482"
     CDS             complement(2033..3031)
                     /gene="tolA"
                     /locus_tag="ACF2SP_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFD2360256.1"
                     /translation="MISDLTYRKAFLFAVTLHLFLLVLLFTDNSSKRPVLTMESKNSP
                     GMMQPTASQQEVVKAVSVDNKEIMKTVNRLKQEREQQKKAELNRQKELLRQAEAAKQQ
                     RIKEQQEIARLKEEANKIAIARKKQVEEEKKRLKELAEQKALEAKRIEELKKQNEKLA
                     KQRELEAKKLAELNKKKQEEKEKAEKLKAEQAKAEKLKAEQARAEQEKAEMERKRQAE
                     AAAAQQAAQNAERQARIAGEVDKYKALIVNAIGRNWILPENVDSSLSSQFRIRLAPDG
                     TVLEVSLIRSSGDPLLDRSAQTAIYKASPLPVPQDVETFNLFRDISLTVRPEQVRG"
     gene            complement(3028..3483)
                     /gene="tolR"
                     /locus_tag="ACF2SP_002483"
     CDS             complement(3028..3483)
                     /gene="tolR"
                     /locus_tag="ACF2SP_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HFD2360257.1"
                     /translation="MNRSKTNRERPISEINVVPYIDVMLVLLVIFMITAPMLTQGVTV
                     DLPKAASQTLQAADREPIIVSVNQLGSYFLNISSTPAEPIEAQALLVRVAAELELAKQ
                     SGQKLNVLVKGDQGVAYGKVVQAMALLKQAGAEQVGLLTDSQTGDEGQA"
     gene            complement(3492..4169)
                     /gene="tolQ"
                     /locus_tag="ACF2SP_002484"
     CDS             complement(3492..4169)
                     /gene="tolQ"
                     /locus_tag="ACF2SP_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HFD2360258.1"
                     /translation="MVANQANVLMYFMQAGLVVKSVMLILLIASIISWTLIFQRAWFF
                     NRRKQMTETFTRRFWDCGDLSKLYADVDSNSDERQGVAAIFHAGFKEFVRCRKQGKVS
                     IEPIQRVMQISHAKEAEKLEQHLPFFASVGSISPYVGLFGTVWGIMTSFQALGHAQQA
                     TIAMVAPGISEALVATALGLFTAIPAVIAYNRYTTRANTLLNKYDLFQEELISLIEQQ
                     TSGSPRG"
     gene            complement(4194..4589)
                     /gene="ybgC"
                     /locus_tag="ACF2SP_002485"
     CDS             complement(4194..4589)
                     /gene="ybgC"
                     /locus_tag="ACF2SP_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFD2360259.1"
                     /translation="MNLISNHQHNIRVYAEDVDYMGIVYHANYLCYLERARTELLRQN
                     NLILSEFEKSDILFAIHELTINYVFPARLDDQLTIKTEIKEFRACSFLFNQEISNQHK
                     QLICRASVQVVCVGSNLKPKRLPNIMRNE"
     gene            complement(4593..5603)
                     /gene="ruvB"
                     /locus_tag="ACF2SP_002486"
     CDS             complement(4593..5603)
                     /gene="ruvB"
                     /locus_tag="ACF2SP_002486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFD2360260.1"
                     /translation="MLESDRLISSQSIVSEDAMDRAIRPLSLSEYVGQDSVSSQMQIF
                     INAARKRNDPLDHVLIFGPPGLGKTTLANIIAHEMGVNIRQTSGPVIERAGDIAAILT
                     NLQQNDVLFIDEIHRLSPVIEEILYPAMEDYKLDIMIGEGPAARSIKLELPPFTLIGA
                     TTRAGLLTSPLRDRFGIVQRLEYYSVDSLTKIVARSAHLLGVPTKPEGAREIALRSRG
                     TPRIANRLLRRVRDYSEVKGNGIITVDMAQQALEMLEVDQHGFDLMDRKLLLAVIEHF
                     NGGPVGIDSIAAAIGEEKGTIEDVLEPFLIQQGFLMRTPRGRIATSKAYQHFGFSAIE
                     QE"
     gene            complement(5750..6313)
                     /locus_tag="ACF2SP_002487"
     CDS             complement(5750..6313)
                     /locus_tag="ACF2SP_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFD2360261.1"
                     /translation="MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTH
                     DWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKITQNTIKNYFRTNNLYTDSEMQY
                     MDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
                     CPIGTVRSRIFRARKQLMDIVSENHTL"
     gene            complement(6414..6857)
                     /locus_tag="ACF2SP_002488"
     CDS             complement(6414..6857)
                     /locus_tag="ACF2SP_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1578 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360262.1"
                     /translation="MRNPKKSCLKLYHSSETVIKPGKSKLYLRLIAILYLCTIGLVLY
                     SSLPVLIEGVLIILIVIQFKNDVTYQSACQKVKEIKFLKNLTWSLETIDGTTHHYDKL
                     KILIHNTLFQVIQLSSDKKNQMLILFNDQIPTDQLRLLHLNSNKK"
     gene            complement(6799..7038)
                     /locus_tag="ACF2SP_002489"
     CDS             complement(6799..7038)
                     /locus_tag="ACF2SP_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFD2360263.1"
                     /translation="MDNREKSRLLWKCRRGMLELDLLLQKFIANEIDRLTENQLKAFD
                     NLLTHNDPSLYAWLMGHEEPEKELLEIVSFIRNCD"
BASE COUNT         2090 a         1477 c         1269 g         2388 t
ORIGIN      
        1 tatctgtttt tctgtaatat tttgagtatt taactgatat tgcttgccgg gcacctgatg
       61 agctgaaacc ttataaactt tagtcaatgc caattgatgt gatgcattaa tccattgttg
      121 cggatttttt tccttactgg ctgattggca agaccatttc ttttccaatt gtctcgcctt
      181 gtttttaatc tgaaaaaact tgtcttgacc aatcagaata ccgccaccat tatcccaata
      241 ttgatgcaaa ttattcccaa taggattttt agctaactga aataaattac cacccaaatc
      301 acctttaggt aattttttac ttatctttgt aacggtatgc aaaggctgat gaatatctcc
      361 aaccaaatgc accaatatgc gtaagctcaa ttttttatca gcaatactgg ctttattgga
      421 agacagcaca gcaattgcct gattaattcc ccagagtgca ttgatgtctg gcaaggctgg
      481 taattctgtt tcatccatag aaaaaggaat gtcaatgtaa tgtaaagcat caaaccaatg
      541 aacatcatgt gctcttatag aatccagcca tgaagctgat tttacaaaat tgacattaga
      601 cgacgtcttc gattttgaat gggaatataa atcacacatt ctcctggatt gaggtgtcaa
      661 attgtcatat gcgatctggg cgaccagttg gtgccctatc gcgttccatg cataaccagc
      721 attagcaata aataaaaaaa acaataccaa tatgcttctt atcattctta actcaggtaa
      781 ggggaccaag cgggctcttg aacatctcct tcacgagcag gaagtctcat tctaattctc
      841 ccgtcaattg acacaatgcc taaaacccct ctgtcctgat aacgggtagc ataaagcact
      901 aaacgactat ttggagagac tgatggtgat tcatcaaggc cagaaaaggt taaattggaa
      961 atcgggccac cacctgtatt ttgtacacca atattaaatt gtctatcctc cctgtgcagc
     1021 atgacgatat gcttcatatc tggagtgtag gaggcgcggg cattataatt gccctcgaaa
     1081 gtgactcgac ttatttggcc atcggccaat gataagcgat atacttgcgg tgacccgcct
     1141 ctccctgaag tgaacagcaa actgcgacca tcaggagaat aacgtggttc cgtatcgata
     1201 gaattaccaa aagtaagctg tttcatagaa ccgctgctca aatcaacatc gtaaattttg
     1261 ggtgtaccgc ttttggacaa tacaactgcc aaatgccgac cgtcaggtga ccatgcgggc
     1321 gctccattaa ttcctgggaa gcttgtaatt aagcgacgct gtcctgtttc aacggagacg
     1381 gtaaaaattt cagctttttt cttttcaaaa gaaacatagg atatcgactt tccattgggt
     1441 gaccatgaag gtgacataat aggctctgaa gagacgagta agctttgagg attatgtccg
     1501 tcagcatcag caacctccag ggagtagcga gaccttcctc cgtttctttg taccgaaata
     1561 taagctatcc tcgtggagaa aatcccccgt tctccagtca gtttttggta tacctcatca
     1621 ctaatatgat gagccaaagc tcttacctga ttggcattga tctgaaaagt ttttgtcaac
     1681 aaaatattgc cattggctac ggcatcggcc aaagtaaagc tcacttcaat ccggttacca
     1741 acctgattaa ctcggccagt gactacactg tcagcgccaa gttgacgtaa tgtactcacg
     1801 gaagattgag aattcgcgcc ttgtggtcca gagataattt taaattgtcc tgatatagtc
     1861 aaatcattct ctatgacatt tcctatttcc tgagccgccg cgtctgagcc aaaagaattg
     1921 atggctatgg gcagggctga attaattcct tgagttagtt ccaaatcaag agcaatcact
     1981 tgaccggtaa ataacagcaa gaaaagtgaa ataattcgat tgcacacgag ctttaccccc
     2041 taacttgttc tggacgaacg gttaaactga tatcgcgaaa caaattaaat gtttcaacgt
     2101 cttgaggcac gggaagtggt gaagccttgt atattgctgt ttgtgctgac ctgtctaaca
     2161 aaggatcacc actgctacgg attagagaca cttccagtac agttccatca ggagcaaggc
     2221 gtatcctgaa ttggctcgac agagaactgt cgacattttc aggtaaaatc cagttgcgtc
     2281 cgatcgcatt gacaataagt gccttgtatt tatcaacttc accagctatc cgtgcttgtc
     2341 tttcagcatt ttgggctgcc tgttgggctg cagccgcttc tgcttgtctt tttctttcca
     2401 tctctgcttt ttcttgctca gcacgggctt gttccgcttt cagcttttcg gccttcgcct
     2461 gttcagcttt aagtttctcg gctttctctt tttcttcttg ttttttctta ttgagttcag
     2521 caagtttttt agcttccaat tcccgttgtt tggccaattt ttcgttttgt ttctttaact
     2581 cttcaatacg tttcgcttct aatgcttttt gctcagccaa ttcttttaac cgcttttttt
     2641 cttcttcaac ttgttttttt ctggcaattg caattttatt agcttcttct ttcaatcttg
     2701 caatttcttg ttgctctttg attctttgct gttttgcagc ctcagcttgc cttaataatt
     2761 ccttttgtcg attaagttca gctttcttct gttgttcacg ttcttgtttc aaacgattaa
     2821 cggttttcat tatttcttta ttgtcgacac tgaccgcttt tactacttcc tgctgagaag
     2881 cagtaggttg catcatgcca ggagaatttt tggattccat ggttagcaca ggtcttttgc
     2941 tgctattatc tgtaaaaagc aatacaagta aaaaaagatg cagtgttaca gcaaaaagaa
     3001 aagcttttct ataggtaaga tcacttatca tgcttgcccc tcatcaccag tctgagagtc
     3061 agtaagcaat cccacttgct cagctcctgc ttgctttaag agtgccatgg cttgaacaac
     3121 tttcccataa gcaaccccct gatctccctt aacaagcaca ttgagttttt gaccagactg
     3181 tttggctaac tccagttctg ccgcaactcg tactaataat gcctgagctt caataggttc
     3241 tgctggcgta ctactaatat ttaaaaaata gctgcctaat tgattcactg atacaatgat
     3301 aggttctctg tcagcagcct gtaaagtttg actggctgct ttgggcagat ctactgtaac
     3361 accttgtgta agcattggag cagtaatcat aaaaataacc agtaaaacca acataacatc
     3421 aatgtaaggc actacattaa tttctgagat tggacgttca cgattggttt tagatcgatt
     3481 cattttaagt cttatcctct aggtgaacca gaagtttgtt gttcaattaa agaaatcagt
     3541 tcttcttgaa ataaatcata tttattcagc aaagtattag cacgtgtcgt atatcggttg
     3601 taggcaataa ccgcaggaat tgcagtaaat agccctaaag cggtagcaac cagcgcttct
     3661 gaaatacctg gtgctaccat tgcgatagtg gcttgttggg catgtcccaa agcctgaaat
     3721 gatgtcatta taccccaaac tgtgccaaat agacccacgt agggagaaat agatcctaca
     3781 gatgcaaaaa atggtaaatg ttgctccagt ttttccgctt ctttggcatg acttatctgc
     3841 attactcttt gtataggctc aatagatact ttgccttgtt ttctgcagcg aacaaattct
     3901 ttaaaaccag cgtgaaagat agcagccacc ccttgtcttt catcactatt actgtctaca
     3961 tcagcataga gtttgctcaa atctccacaa tcccagaacc ttctggtaaa ggtttcagtc
     4021 atttgctttc tacgattaaa gaaccatgct ctttgaaaga ttaatgtcca tgaaattatc
     4081 gaagcaatta ataatatcaa cataacggac tttactacca aacctgcttg cataaaatac
     4141 attagtacat ttgcttgatt agccaccatt aatctccaca ctgaaaggtt tcgttattca
     4201 tttctcataa tattaggcag tcgcttgggc tttaaattac tgccaacgca aactacttga
     4261 acactggccc tgcaaattaa ttgtttatgt tgattagata tctcttgatt aaatagaaaa
     4321 ctgcacgctc taaattcttt aatttctgtt ttaattgtta attgatcatc taacctggcc
     4381 ggaaatacat aattaattgt gagttcgtga atcgcaaata aaatatcgga cttttcgaat
     4441 tcacttagaa ttaaattgtt ttgccttaat aattcagttc gagctctttc caaataacaa
     4501 aggtagttag catgataaac aattcccata taatcaacat cttctgcata gactctaatg
     4561 ttatgctgat gatttgatat aagattcatg tgttattcct gttcgatcgc actaaagcca
     4621 aaatgctgat aagctttcga agtggctatt ctaccgcgag gagtgcgcat tagaaagcct
     4681 tgttgtatta aaaatggctc taaaacatct tctatagtcc ctttttcttc gccaatagca
     4741 gctgcaatac tatctatgcc tactggacca ccattgaagt gttctatcac cgctaacaat
     4801 aattttctat ccattaaatc aaacccgtgc tgatccactt caagcatttc caatgcttgt
     4861 tgggccatat caacagtaat gatgccattt cctttcactt cagaataatc cctgactctt
     4921 cttaacaggc gattggctat tcgtggtgtt cctctggagc gtaatgcaat ttctcttgct
     4981 ccttcgggtt ttgtagggac accaagcaaa tgcgcagagc gtgccacaat cttggtaagc
     5041 gaatcaacag aataatattc caaacgttgc actataccaa atctatccct taatggcgag
     5101 gtaagcaacc ctgctctcgt cgttgctcca attaatgtaa agggaggtaa ctccaattta
     5161 atagatcgtg ctgctggccc ctctcctatc atgatatcca acttataatc ttccatggct
     5221 ggataaagaa tttcttctat aacaggactt aatctatgaa tttcatcaat aaatagtaca
     5281 tcattttgtt gtagattcgt caatatagca gcaatatctc cagcacgctc aatcactggc
     5341 ccagaagttt gtcggatatt cactcccatt tcgtgagcta tgatatttgc aagggtagtt
     5401 ttaccaaggc caggtgggcc aaaaataaga acatgatcca aaggatcgtt tctctttctt
     5461 gccgcattga taaatatctg catctgtgag ctaacagaat cttgccctac gtactcgctc
     5521 aagctaagcg gcctgatagc tctatccatt gcgtcttccg agacaatact ttgactactt
     5581 attaggcgat cactttcgag cataagaggt tttatcaggt tcattcgaat gaaagcattt
     5641 taacataggt ttatctaata gcagatcttt gtaagcttat ttatttaata attaaatgca
     5701 attgatttca cgattaaggt tgatgatttt atatgatatc aaaatgatat tataaagtgt
     5761 gattctcact tacaatatcc atcagctgtt ttcgagctct aaatatacgt gagcgtacag
     5821 ttccgatggg gcaatgcatt tcttttgcaa tatcctgata agtttgtcca tcaaatatgt
     5881 gcagcccata acataatcgt aactcttcag ataaacatga cattgctgct tctatttgtt
     5941 tctcaaactc taatcccatt atgtctgact ctggagaatt atgtggtgta gaaaattgtt
     6001 catccatata ctgcatttct gaatcagtat ataaattatt ggtgcgaaaa taatttttaa
     6061 tagtattttg agtaatttta tatagccagg ttgaaaactg actgcgttca ttaaaataag
     6121 gtaaatgcct aaatactttg ataagaactt cctgcgttaa atcattgaca tttgtccagt
     6181 catgcgtgta aaaataaatc atttgtctaa ttttgccctg ataacgctca aacagtttat
     6241 caaaagctag accgttgcct ttttgagcta attcaattag agattcatca cttaaagagg
     6301 tctcaatatg catagcttcc tcattattac tttgcttgtt atttcgttag gataagataa
     6361 agtctaacca ttgttaattt gaatttattt taatcattat gtattattat atactacttt
     6421 ttattgctgt ttaggtgtaa taatcgtaat tgatcggtag gtatttgatc attaaataga
     6481 attagcatct gatttttttt atctgaagat aattgaataa cttgaaatag cgtattatga
     6541 ataagaattt ttagtttgtc ataatgatgg gtagtaccat ctattgtttc cagtgaccac
     6601 gtaaggtttt tcaagaattt gatttcttta actttttggc aagcactttg gtaagttaca
     6661 tcatttttaa attgaataac gataagaata attaagacgc cttctatcaa cacaggtaaa
     6721 gaagaatata aaactaatcc aatggtgcac aaataaagaa tcgcaattaa tcgaagatat
     6781 agtttggatt tacctggttt aatcacagtt tctgatgaat gatacaattt caagcaactc
     6841 tttttcgggt tcctcatgcc ccattaacca ggcatataaa ctagggtcat tgtgggttaa
     6901 tagattatca aatgctttca attgattttc tgtaagtcga tcaatctcat tggctatgaa
     6961 tttctgtaaa agaagatcaa gctctagcat tcctcgcctg catttccata ataaccttga
     7021 tttttctcta ttgtccacaa tggatttcct ctgttacttc acgataaata caacataatc
     7081 ctaattattc aataatgata tctgattttg agaggccaaa tgatacacta gtctgtatta
     7141 taaacaacat cattgatctt gatatgaaaa ctatattaga acatttagtg aataataggt
     7201 ctttaataac attccgccct atag
//
LOCUS       DAXLDT010000147         7215 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXLDT010000147 DAXLDT010000000
VERSION     DAXLDT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.147"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(37..687)
                     /locus_tag="ACF2SP_002490"
     CDS             complement(37..687)
                     /locus_tag="ACF2SP_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFD2360264.1"
                     /translation="MTYSFSKPVSWVFLFTAVIYLVSLSFIQYPVTTVLKPIPIVCLI
                     VGVFRTFLPSLAKIFLILALVFSLAGDVVLTLPVALQLELGIGCFLLAHCFYIALFLK
                     SFQFNKLHFFYYILILLLMGFIAFTIIPYLGDLLIPVMIYFCVLMLMVFSAFQVKKET
                     LTIGSGALFFLISDLTLALNLFVYTQADIRIFIMFTYYVAQFLLTFGLVRLYEEGD"
     gene            complement(767..1267)
                     /locus_tag="ACF2SP_002491"
     CDS             complement(767..1267)
                     /locus_tag="ACF2SP_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360265.1"
                     /translation="MTKEKSESGNSPSNSEEHGTLGPDVYENARKLNIQLATRGPAIL
                     YALGKASEQVTNGNVVTAVGGMVVGAIGFFGNRFYRNKGDDSAYTYMGQIVGAVQFTA
                     GFTLLALQAFEAFTDTGETSPLLNVVKLSGVALTLTESALILINSYLYGDGSAKTTSI
                     TSPKID"
     gene            1759..2619
                     /locus_tag="ACF2SP_002492"
     CDS             1759..2619
                     /locus_tag="ACF2SP_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFD2360266.1"
                     /translation="MLKRLLGVASLFLILNHQCFAEDHEIAITIDDLPFVGSGTSTPG
                     NLKRTQERFMAIVNTLVDNQVPATGFAIGGAIAKNEWELLEIFRNQGFSIGNHTYKHR
                     SLNSMTAENYIADIEKADTVLSPVMTEPKYFRYPYLAEGSGEKKQKVHEWLAAHQYTI
                     APVTIDSKDYEFNAQFYRIPYRQRPQRLAQFKKRYLAFIWQQTLRAEKKVKKVEGQPV
                     KHILLIHANLINSLCLADIIEMYRSNGYKFITLQEALKGNTATPINDSSPTEALKSET
                     EKKEINEPQS"
     gene            complement(2737..3933)
                     /locus_tag="ACF2SP_002493"
     CDS             complement(2737..3933)
                     /locus_tag="ACF2SP_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFD2360267.1"
                     /translation="MKSKLIYLTVALAAIILSAAVWWWLQSPKPTFRQVSFKHLPGWE
                     NAQLKKSLETFQTSCRAFLKQNPERVVGTEQIDLQAKDWHPACRAALKINPVDEQSAK
                     VFFEKWFAPVEFYDDKPVKGLFTGYYMPLIKGSYTKSEEFSVPIYELPSNLVTVDLGL
                     FLPNLKNKKIIGRIAGNKVVPFYTREEINKGAIKNTARVLVWIHSPVDRLFLEIQGSG
                     IIELEDGKRIFIGYDGQNGAPYTAIAGVLIKKGIMTKHNASMQRIKKYLEAHPKQMDK
                     IINQNKSFVFFRKLSLEAALGSQGVALTPGYSLAIDKQWIPMGAPLWLNTTRPDSKNP
                     DVNKPMQRLMIAQDTGGAIRGKVRGDVFWGGGDKATLIAGHMKNEGHYWLLLPAHALS
                     RLAQLK"
     gene            complement(4043..4600)
                     /locus_tag="ACF2SP_002494"
     CDS             complement(4043..4600)
                     /locus_tag="ACF2SP_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360268.1"
                     /translation="MKKTDSGFILLMTLCITFLMSLLVLASLQQIFLQYKALNSQETF
                     HQNFYQLEHWMMRLAHSPLLYAGMDCYLQKDYGANKIANELVNNKGCLLILNQRKYRY
                     FIEDLNEFSCLVLNKDGQKYASHHFRFTVLQDEEHGESAIMQLRFIKLGSNLSSCPEE
                     EIQVKEGVSSWRYLPDYKNFEILTG"
     gene            complement(4597..5403)
                     /locus_tag="ACF2SP_002495"
     CDS             complement(4597..5403)
                     /locus_tag="ACF2SP_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFD2360269.1"
                     /translation="MLRGLKKASQLFAPMIWVLIEMGRQTGFSLSEMLISLFLASIIT
                     ITLTQFYIRSKPQYLKAQERLEINFDLLWIGDLLRDSIRKAGFTPCVSVEKLKTFDSR
                     HAERKIINFQIENMPQVQLRINRMSDVYTEVTNFMSPTQIVIEESVISNIKHPLIISD
                     CRQGEVHTVSKIDKLSNGYQITLKKPLRFRYDEITYLGEWLEETWFTKQNEKGEKTLH
                     YKLFQTEELSPFIHEFYVTRRLIKQRSVLDFSIGSDKETLRKISVSVRNL"
     gene            complement(5342..5656)
                     /locus_tag="ACF2SP_002496"
     CDS             complement(5342..5656)
                     /locus_tag="ACF2SP_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFD2360270.1"
                     /translation="MSKHKGFTLMEVLMSLMLMTTVVLMLVDQQLKTRDLINHLIWRI
                     QGSQYLDQVDEALYISENRLPLPPSNYHFDIRRSQKKIILNVTWFKKSQSIIRSYDLG
                     AN"
     gene            5816..6925
                     /gene="hisC"
                     /locus_tag="ACF2SP_002497"
     CDS             5816..6925
                     /gene="hisC"
                     /locus_tag="ACF2SP_002497"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFD2360271.1"
                     /translation="MSIDFQQLPHAGIRSLIPYVPGKSIEELAKEKGITDIIKLASNE
                     NPLGCSPLALSAIQTMSSHYIATYPSPWNHPLMSKLASYLKVKPEQLFLSNGSDYLFN
                     ILLNCFALHTDRHILTHDYAFSTYAIQANSLQIPINSVPIGHNWEVNITDIVNACNQQ
                     TGIIFIANPNNPTGMLIQQEEIKYLLEQIPKSTLLVLDEAYYEFAASQLTVNSLDWLE
                     EHPNLVVTRTFSKIYGMAGLRLGYAIANPSIINILKRVQLPFIVNQVALAAAYAAIDD
                     DDFIQSSLKMNNEGMSQLQAGFNELNIKYLHSSCNFLTFDCEEDSIALYNYLLDNGII
                     VRPLHAYKMNNFIRVTIGTKEQNSRFLTALKNFYL"
     gene            6962..>7215
                     /locus_tag="ACF2SP_002498"
     CDS             6962..>7215
                     /locus_tag="ACF2SP_002498"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HFD2360272.1"
                     /translation="MTSDLSSKHCESCEGIGAALNSEQIKNLLPQLNTKWEVTEDNRI
                     IKRAFSFKNFYETMAFVNAIAWIANIENHHPDLEVGYNYCR"
BASE COUNT         2367 a         1400 c         1183 g         2265 t
ORIGIN      
        1 atgaacattt tttatgttat ctgaataata aattcatcaa tccccttctt catataatct
       61 gacgagacca aaagttaata agaattgagc cacataataa gtaaacataa taaaaattct
      121 tatatcagct tgggtataaa caaacaagtt aagtgccagt gttaaatcag aaattaaaaa
      181 gaacaaagct ccactcccaa tcgttaaagt ttcctttttt acttggaaag cactaaatac
      241 cataagcatc agaacacaga agtaaatcat tactggaatt aataaatccc caagataggg
      301 gattatggta aatgctataa accccattaa tagaagaatg agaatataat agaaaaaatg
      361 taatttatta aattgaaatg attttaaaaa caaagcgata taaaaacaat gcgctaacaa
      421 gaagcaacct atccctaatt ctagttgtaa tgcaacaggt aaagtcaata ccacatcccc
      481 tgccaaggaa aatacaagtg ccaagattag gaaaatttta gctaatgagg ggagaaaggt
      541 tctgaatacc ccaacaatta aacaaactat aggaataggc tttaaaacag tagtaacagg
      601 atattggata aacgaaagac taaccaaata aatgactgcg gtaaacaaaa acacccaaga
      661 cactggtttg gaaaaagaat aagtcataaa tagtccctta ctgtaaaatc aaagtcattg
      721 atttgagctg ccttatgcct caaccctact agccttttta ttgtattcaa tcaatcttag
      781 ggcttgttat tgatgttgtt ttggctgatc catccccata taaatacgag ttgattaaaa
      841 ttaaagcgct ctcggttaat gttaacgcaa ccccgcttag tttaacaaca ttgagtaagg
      901 gacttgtctc tccagtatct gtaaacgcct caaatgcctg taatgccaac agtgtgaatc
      961 cggcagtaaa ttgcaccgca ccaacaattt gtcccatata agtatacgcg gaatcatcac
     1021 ccttgtttcg ataaaaacga ttaccaaaaa aacctatcgc cccaacaacc atgccaccaa
     1081 ccgccgttac aacatttcca ttagttactt gctcactggc tttacctaat gcatacagta
     1141 tggcaggccc tcttgttgcc aattgaatat ttagctttct tgcattctca taaacatccg
     1201 ggcctaaagt accatgttct tctgaatttg atggtgaatt tcctgattca gatttttctt
     1261 ttgtcattat caactcctgt ttaacaaaat tccgggtaag acagtatcct ttcaatttct
     1321 cagattatat ccacggcctt gcgttattgt ttgacatgat cagctctaat ttcacgtgag
     1381 ttatggataa gatcaaccgt tctcttgaat cgctcaggct gaggagatat ttatgccgtc
     1441 tcaaagcctt gtatggaatt taatatttca tacttaggct ttgagaggat gcttacacat
     1501 ctccctgacc caaacggacc ctggatatcg agtgctccgt atataaccct tatgcataac
     1561 tcacgttaat ttaagtaatt ctctatttgt cactttaata aattatccaa tagccgaaaa
     1621 atcgaagtat ttattgtacc taaaccctct caaaacacat acatatttcc aaacaggtgg
     1681 aaatttaatt tatcgtctat agttaaatta aatgcctaaa ataataagaa attttccaaa
     1741 ttgaaaagag gggaaaatat gttaaaacgt ctactcggtg tagcatcatt atttttaata
     1801 ttgaaccacc aatgcttcgc tgaagatcat gaaattgcca ttactattga cgatttacct
     1861 tttgtgggct ctggaacaag tactccagga aatctgaagc gaacccagga aagatttatg
     1921 gccatagtga acactttggt tgacaatcag gttcctgcca caggttttgc cataggaggg
     1981 gctatagcga aaaatgaatg ggagcttctg gaaatttttc gtaatcaagg tttttcaatt
     2041 ggcaatcata cctataagca tcgaagttta aattcaatga ctgcagaaaa ttatattgct
     2101 gatatcgaaa aagcggatac cgttctatcc cctgttatga ctgagccaaa atatttccgt
     2161 tatccttatc ttgctgaagg cagtggagaa aagaagcaaa aagtgcatga gtggctggct
     2221 gcccatcaat acacgattgc tcctgtcact atagatagca aagattatga attcaatgcg
     2281 caattttata gaataccata tagacaaaga ccacaacgtt tagctcaatt taaaaaacgc
     2341 tatcttgctt ttatctggca acagaccctg agagcagaaa aaaaagtcaa gaaagtagaa
     2401 ggacaacctg ttaaacatat tttactcatc catgcgaatc tgattaatag tttgtgtttg
     2461 gctgatatca ttgaaatgta tagaagcaac ggttataaat tcattactct gcaagaggct
     2521 ctaaaaggga atacagctac tccaattaat gattcatcac caactgaagc tttgaaatcg
     2581 gaaaccgaaa agaaagaaat taatgagcct caatcttaaa aagtatagag ttatttcttg
     2641 aaacaaatga tttacaatgc tatcagggat tcattgtcac aggactggtg aatcccaata
     2701 gccaaataaa aaattgcaaa gacaggactt acgcccctac ttcaactgtg ccaagcgcga
     2761 taatgcatga gccggcagaa gaagccaata atgtccctcg ttcttcatat ggccagcaat
     2821 caaagtggct ttgtcaccac cgccccaaaa tacatcacct cttactttac cacgaatagc
     2881 cccaccagta tcttgagcta tcatcaaacg ttgcataggt ttattgacgt caggattttt
     2941 actatctggt cttgtagtgt ttaaccacag aggcgctccc attggtatcc attgtttatc
     3001 aatagcaaga gaataaccgg gtgttaatgc cacgccctgt gaacccaaag ccgcttctaa
     3061 tgacaacttg cggaaaaaaa caaatgattt attttgattg attattttat ccatttgctt
     3121 gggatgagct tccagatact ttttaatcct ttgcatggaa gcattgtgct tggtcataat
     3181 acccttttta attaatactc cagcaatggc agtatagggg gcaccatttt gaccgtcata
     3241 gcctataaaa attcgtttgc catcttcaag ctcaatgatt cctgaaccct gaatttctaa
     3301 aaacaacctg tccacggggc tatggatcca caccaaaaca cgtgctgtgt ttttaattgc
     3361 tcctttgtta atttcctctc gagtataaaa tggtacaact ttattacccg caattcgtcc
     3421 aataattttc ttatttttta aattaggaag aaataaaccc aagtcaacag tcactaagtt
     3481 actgggtaat tcataaatag gaacactaaa ttcctctgat ttggtataac tgcctttaat
     3541 taaaggcata taatagcccg taaataaacc tttcactggt ttatcatcat agaattctac
     3601 aggggcaaac cacttctcaa aaaatacttt tgctgattgt tcatcaacag gatttatttt
     3661 taaagcagca cgacaggctg gatgccaatc ttttgcctgt aaatcaatct gctctgtgcc
     3721 aacaacacgt tcaggatttt gttttagaaa ggctcgacaa gatgtttgaa aagtttctaa
     3781 cgatttttta agttgtgcat tctcccaccc aggtaaatgc ttaaagctta cctgcctgaa
     3841 agttggcttg ggagattgca accaccacca aactgctgcg cttaagataa ttgcagccaa
     3901 ggctactgtc agataaatta atttcgattt cataggaggt caatttttac acgtgatgga
     3961 gaaaaatgca actaaaaatt attgttatta gtccatctta ctacaaaaaa catccggtta
     4021 taatagcttt ttttcaataa tttcaaccag ttaatatttc aaaattttta taatctggca
     4081 agtatctcca gctgcttacc ccttccttca cctgtatttc ctcctctgga caagaggaca
     4141 aattgctacc caatttgata aaccttaatt gcatgatagc agattcgcca tgttcctcgt
     4201 cttgtaatac ggtgaaccta aaatggtgag aagcatattt ttgcccatcc ttattaagaa
     4261 ccaaacaaga aaattcgttc aggtcctcaa taaaatatct gtattttctc tgatttaata
     4321 tgagcaaaca tcctttattg ttaactaact cgtttgctat tttattagct ccataatcct
     4381 tttgcagata acaatccata cctgcataca ataacggtga atgagcgagt cgcatcatcc
     4441 aatgttccaa ctgataaaaa ttttgatgaa atgtctcctg tgaattgagt gctttgtatt
     4501 gcaagaatat ctgttggagg cttgccaaaa ctaataagct catcaagaaa gttatacaga
     4561 gcgtcattaa cagaatgaaa ccggaatcgg tttttttcat aaatttcgta ccgaaacact
     4621 gattttacgt agcgtttcct tatcgctacc aattgaaaaa tccaatactg acctctgttt
     4681 gataagacgt ctggtaacat aaaattcatg aatgaatgga cttaattctt ctgtttgaaa
     4741 cagtttgtaa tgtaatgtct tttcaccctt ttcattttgt ttagtaaacc atgtttcttc
     4801 aagccactct cccaagtaag taatttcgtc gtatcgaaac cgcagtggtt tttttaaagt
     4861 aatttgatat ccattgctta gtttatctat ttttgaaact gtatgtactt ctccttgccg
     4921 gcaatcagaa ataattaatg ggtgttttat atttgatatt actgactctt caatcactat
     4981 ttgtgttggg ctcataaaat ttgtcacttc ggtataaaca tcactcattc tgttaatacg
     5041 aagttgaact tgtggcatat tttcaatttg aaaattaatt attttcctct ctgcatgtcg
     5101 agaatcaaaa gtttttaatt tttcgacact tacgcatgga gtaaatccag cctttctaat
     5161 actgtctctt agtaaatcac caatccacag caaatcaaaa ttaatttcta accgttcttg
     5221 tgcctttagg tattgaggct tgcttcttat ataaaactga gtaagggtta tcgttataat
     5281 acttgccaga aaaagactaa ttagcatctc agataaacta aatcctgttt gtcttcccat
     5341 ttcaattagc acccagatca taggagcgaa taattgactg gcttttttta aaccacgtaa
     5401 cattcaaaat gattttcttt tgagatcgcc tgatatcaaa atgataattt gaaggcggca
     5461 gagggagtct attctcacta atatataatg cttcatctac ttgatctaga tattgggaac
     5521 cttggattcg ccagataaga tgattgatta gatctctggt tttcaactgt tgatccacta
     5581 acatcagaac taccgttgtc attagcatta atgacataag tacttccatt agagtgaaac
     5641 ccttatgctt gctcataata aatctcttcc tgaggttgga ctgcccatga aggttaactg
     5701 ttcgtttata taaattcaat ataacgattt ataaattcta aaaaataaat tcattcgtga
     5761 cagctggcat atgcttaggt aatataaaaa catttttctg tctaggaata gagttatgtc
     5821 tattgatttt caacaattac ctcatgccgg aatacgttct ttaattcctt atgtaccagg
     5881 taaatccatc gaagaattgg caaaagaaaa gggaataact gatattatca agctggctag
     5941 taatgaaaac ccattgggat gcagcccatt agccctttcc gccattcaga ctatgtcatc
     6001 gcattacata gcaacctacc catcgccctg gaaccatcct ttaatgtcca aacttgcaag
     6061 ttacttaaaa gtaaaacctg agcaattgtt tctaagcaat gggtctgatt acctgtttaa
     6121 tattttatta aattgtttcg ctcttcatac cgacagacat atccttactc atgattacgc
     6181 ttttagcact tatgccatcc aagccaattc cctgcaaata ccaataaata gtgtgcctat
     6241 tggccacaac tgggaggtta atatcactga tatcgttaat gcttgcaatc aacaaacagg
     6301 aattattttt atagctaacc caaataatcc aactgggatg ctaatccaac aagaagaaat
     6361 taaatattta ttggaacaaa ttccgaaaag tactttgtta gttttagatg aagcctatta
     6421 tgaatttgct gctagccaat tgactgtcaa tagtttggat tggcttgaag aacatcctaa
     6481 tttggttgtt actcgtacgt tttcaaaaat ctatggtatg gcaggtttgc gtttaggtta
     6541 tgctattgcc aacccatcca ttattaatat tttaaaacga gtacaattgc catttatagt
     6601 caatcaagtt gctcttgcag cagcctatgc tgccatcgat gatgatgact tcatccagtc
     6661 tagcctcaaa atgaataatg aagggatgtc gcagctacag gcgggcttta atgaattaaa
     6721 cataaaatac ttacactctt cctgtaattt ccttactttt gactgcgaag aggatagtat
     6781 agctctttat aattatctat tggataatgg cataatagta aggcctctgc atgcttataa
     6841 gatgaataat ttcatcaggg ttactattgg gaccaaagaa caaaattcca gatttctaac
     6901 agcactgaaa aatttttatt tgtaactctt tttaaattaa aaataaaatt aaggaatcaa
     6961 aatgactagt gatttaagta gtaagcattg tgaatcttgc gagggaattg gcgctgcgct
     7021 caactccgag caaatcaaaa atttactacc tcaattaaac acaaaatggg aagtaacaga
     7081 ggacaaccga attatcaagc gtgctttttc atttaaaaat ttttatgaaa cgatggcttt
     7141 tgtcaatgcg attgcctgga tagcaaatat tgaaaatcac caccctgatt tagaagtcgg
     7201 ctacaattat tgccg
//
LOCUS       DAXLDT010000148         7154 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXLDT010000148 DAXLDT010000000
VERSION     DAXLDT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.148"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(241..798)
                     /gene="frr"
                     /locus_tag="ACF2SP_002499"
     CDS             complement(241..798)
                     /gene="frr"
                     /locus_tag="ACF2SP_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFD2360273.1"
                     /translation="MINEIKQDSEKRMKKTIEALHSDMSKIRTGRANASLLDHVMVDY
                     YGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPM
                     PPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
                     QKLTDRYISEVDAVLAEKEKDLMEI"
     gene            complement(788..1531)
                     /gene="pyrH"
                     /locus_tag="ACF2SP_002500"
     CDS             complement(788..1531)
                     /gene="pyrH"
                     /locus_tag="ACF2SP_002500"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFD2360274.1"
                     /translation="MMNESQPKLKYKRILLKFSGEALMGKSQFGIDPSVLDSLARDIA
                     ELIHMGVEVGLVLGGGNLFRGKALSQAGVGRVTGDHMGMLATLMNALALRDALERIDL
                     PARIMSAIPMLGVVDPYHRRKAITHLRNGQVVIFAAGTGNPFFTTDTAACLRAIEIGA
                     DIVLKATKVDGVYSADPLKNPDAKRYDYLTYKEVLTKGLEVMDSTAICLCQDQGMPLQ
                     VFDMAAPNALKRIVTGEQVGTIVGANHDQ"
     gene            complement(1528..2406)
                     /gene="tsf"
                     /locus_tag="ACF2SP_002501"
     CDS             complement(1528..2406)
                     /gene="tsf"
                     /locus_tag="ACF2SP_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFD2360275.1"
                     /translation="MSTISAALVMQLRERTGAGMMECKKFLIATNGDIEQAIIEMRKA
                     GQAKADKKADRVAAEGIIVIARSSDERTAVMLEINSETDFVARDENFTNFANAVADAA
                     LTNLPKNIEDLSNQALSSGTTVEQARQELVAKIGENIKLRRLERMHCDGVIGYYLHGS
                     RIGVMVALKNGSEALAKDIAMHVAASKPMVVSRDQVPAEAIENEREIFTAQAKESGKP
                     QEIIDKMIDGRINKFIDEVSLLGQPYVKDPNIKVGQLLKEKNAEVISFVRYEVGEGIE
                     KKEDNFVEEVMAQVRT"
     gene            complement(2472..3236)
                     /gene="rpsB"
                     /locus_tag="ACF2SP_002502"
     CDS             complement(2472..3236)
                     /gene="rpsB"
                     /locus_tag="ACF2SP_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFD2360276.1"
                     /translation="MNNVSMRELLEAGAHFGHRTRFWNPKMSEYIFGSRNKIHIINLE
                     KTLPMLNDVTNYVSRLAANKAKILFVGTKRAAQDSIREHAKRCGMPYVDHRWLGGMLT
                     NYKTVRQSIFRLKELKEMKEKGLFNDMIKKEALMLTRELEKLERSLGGIENMGGLPDA
                     LFVVDVGFEHIAVEEARRLRIPVIGVVDTNNSPDNIDYVIPGNDDSMRAVDIYVRCVA
                     DAILDGKNSNTVGRVSSDSEFVEVTSNSNEEEKSGE"
     gene            3570..3980
                     /locus_tag="ACF2SP_002503"
     CDS             3570..3980
                     /locus_tag="ACF2SP_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFD2360277.1"
                     /translation="MSKKSICLATLALIFTFGQPVFANSSEATQTKPCHCHDHKKILS
                     RLNLTQEQKTKIKTIKIQAQKSIKENYRQLKAIKEQINALIANEKLDEAKLDSLINQR
                     NKIAGTMLKNRVMMKNQIYNQLTNEQKSQYKKNM"
     gene            4490..4948
                     /locus_tag="ACF2SP_002504"
     CDS             4490..4948
                     /locus_tag="ACF2SP_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1716 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360278.1"
                     /translation="MRGHMIFLSIPKGMEFKQITEKDNTNDYFVDPNGKLPRINIQAL
                     VKDALQYNKGRKKEISLPDFTIYRHKPPYRDELFLQYNPDHNGKYFTKESVNLVNGKE
                     FIKYKTPATSYGTFWFQKVQLSENRMDEVLAKRSEQRENRRHTGDSPNPT"
     gene            complement(5013..6701)
                     /locus_tag="ACF2SP_002505"
     CDS             complement(5013..6701)
                     /locus_tag="ACF2SP_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1717 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360279.1"
                     /translation="MPVRYSGFDYDGCVGVTNLINIVAQNPKLVQRIRETSNYYTKEV
                     ALIASNRQSDRDDKRNARSNGNGSCFIQIQKLCTDLGMEFDPFLLADAYNLVPAGTSL
                     HEATNPANGAKIQNGWLHDKSKTSLVYAHMQRASSLEAREQIDYFFYDDREDILEGLY
                     QFFSANPELIPSNVTMHLVKYACSRENVHTMDNYKIPVQGKGFTNPDYVKTIKAFGDK
                     AIENARDVYDIDGKEINAAGVRQAKYNCNFEFEVNKLQPMKFVNNFNSPAELIKAHEQ
                     AIEKIKLDAFELYKAKINVITTLSNEQKVFLIRAKAQQWNVLCDEGQWNNPIFTLQAI
                     MAEKSDRFFLSQYAFTAKMLIKNYQEARENRKDGSRFWEKINAFIKSMNALKDPMRNA
                     QSIFEVFKKNSYSVKAVDGFVKKVVEVMNALMDKYPQRSTTEMVACMRILESNLQKMV
                     NFFSLVDRLITIYRGCTTAGPNFNKILTVVREALEEMKMITDSGDNYSTTLIDLDKVI
                     TATNNRLESLTYPKEYSTKNFHSQESYKVVGAYLCMQSQLTQKLDTKASTAFAI"
BASE COUNT         2248 a         1483 c         1146 g         2277 t
ORIGIN      
        1 atttattggt actgaggaga gctagctaaa aatgaaacaa atatgctgag agtcaaggca
       61 agatcattcc aactttagtc gattatgccc cgttatactt tataaatttg agcatatttt
      121 tgtcattctc gcacggtcgg gaatgacaaa aatttagcat tatataatca gagtatgttt
      181 tttcctgtac taaaaacaag atctatagct ctattatata aaaggagtaa tgtctacccc
      241 ttaaatttcc attaaatctt tttctttctc agctaatact gcatcaactt cagaaatata
      301 tctatcagtc agcttttgga tcgcttctgc agcacgtcgt tcatcgtctt cagaaattgc
      361 tttttcttta acaagttcct ttagctgatt attggcatcc cttctgatat ttcgaattga
      421 aactcttcct tgttcccctt catggcgaac aactttaatc aattcctttc tgcgctcctc
      481 tgttaaaggt ggcatcggaa ctcgaatggc tgtcccagca gttgcaggat tcaaacccaa
      541 atcagagttt agtatagcct tttctatagc tgcgaccata gatttttccc aaggtgtaac
      601 taaaatagtt cgggagtcac tggtagtaat attggcaacc tggcttaaag gtgtaggact
      661 tccataataa tcaaccatga catgatctaa aagactggca ttcgccctgc cggtacggat
      721 ttttgacata tccgagtgca aagcctcaat agtctttttc atgcgttttt ctgaatcttg
      781 cttaatctca ttgatcatga ttagctccga ctatagttcc tacttgttct ccagttacaa
      841 ttcgtttcaa tgcgttagga gctgccatgt caaacacctg taaaggcatt ccttgatctt
      901 ggcacaaaca aattgcagtg gaatccatca cttccaaacc tttggttaaa acttctttat
      961 aagttaaata atcataacgc tttgcatcag gatttttaag tggatcagca gaataaactc
     1021 catccacctt tgttgctttt aaaacaatat ctgcgcctat ctcaattgcc ctcaaacaag
     1081 cagcagtatc tgttgtaaaa aagggatttc ctgttccagc tgcaaaaatc accacctgac
     1141 cattgcgcaa gtgagtgata gcttttcgtc tatggtatgg gtcaactacc ccaagcattg
     1201 gaatagctga cataatacga gcgggcagat caattcgttc caaagcatca cggagtgcta
     1261 aagcattcat aagagtggcc agcatcccca tatggtctcc tgtcactcgt cctacgcctg
     1321 cctgagagag cgctttccct ctgaacagat tacctccacc taaaaccaaa ccaacttcca
     1381 cgcccatatg aatcaattct gctatatctc gagctaatga atcaagaaca gaaggatcaa
     1441 tcccaaattg agatttcccc atcagcgctt cgccactaaa tttaagcaaa atgcgtttgt
     1501 attttaattt aggttgactt tcattcatca tgtacgaacc tgagccatca cttcttcaac
     1561 aaaattgtct tcttttttct ctattccttc ccctacttca taacgaacaa aggatattac
     1621 ttcagcattt ttctctttta gaagttgacc cactttaatg ttaggatctt ttacataagg
     1681 ttgtcctaac aagcttacct catcaataaa cttattgatt cgtccatcaa tcatcttatc
     1741 aatgatctct tgcggcttac cactctcttt cgcttgagca gtaaatattt ctctttcgtt
     1801 ttcaatcgct tcagctggta cctgatcgcg actaaccacc atcggtttgc ttgcagctac
     1861 atgcatggca atatctttag ccaatgcttc acttccattt tttaatgcga ccataactcc
     1921 aatacgcgaa ccatgcaaat aatacccaat gacgccgtca caatgcattc tttccaatcg
     1981 gcgtaattta atattttcac caattttagc aaccagttct tgtcttgctt gctcaacagt
     2041 agtaccagat gataatgcct gattggataa gtcttctata ttttttggaa ggttggttaa
     2101 tgcagcatcg gcaacagcat tggcaaaatt agtgaaattc tcatctcgtg caacaaagtc
     2161 tgtttcacta ttaatctcaa gcattaccgc tgtacgttca tcagaagaac gtgcgataac
     2221 gattatacct tctgctgcaa ctctatctgc ctttttgtct gcttttgcct ggccagcttt
     2281 acgcatttca atgattgctt gctcgatatc accgttagta gctattagaa attttttaca
     2341 ctccatcata cctgcgcctg tgcgctcacg caattgcatt actaatgcag cactaattgt
     2401 tgacattttg ttttctccac atagcaaaaa gggggcttat taaattaacg ccccctttat
     2461 tcaaatagta attattctcc ggatttttct tcttcattag aatttgaagt gacctcaaca
     2521 aactctgaat cagatgatac tcttcctaca gtattgctat ttttaccatc taaaatagca
     2581 tcagcgacac agcgaacata aatgtccaca gcacgcatag aatcatcatt gcctggtata
     2641 acataatcaa tgttgtcagg gctattattg gtatcaacaa caccaattac cggaattctt
     2701 aatcgacgcg cttcttcaac agcaatatgt tcaaaaccaa catcaaccac aaataacgca
     2761 tcaggtaggc cgcccatgtt ctcgattcca ccaagactac gctccagctt ttcaagctct
     2821 cttgtcagca ttagcgcttc tttcttaatc atgtcattga acaatccctt ttccttcatt
     2881 tcttttaatt cttttaaacg aaatattgat tgacggactg ttttatagtt ggtcagcatg
     2941 cctcctaacc atctatggtc tacataaggc attccgcatc gttttgcatg ctctcgaatg
     3001 ctgtcttgag ctgctctttt agtgcctaca aaaagaattt ttgccttgtt tgctgccaat
     3061 cgactaacgt aattcgttac gtcattcagc atgggtaatg ttttttcaag gttaatgata
     3121 tggattttat ttcttgagcc aaaaatgtat tcactcattt ttggattcca gaaacgagtt
     3181 ctgtgcccaa aatgagcacc tgcttcgagc aattcacgca tactaacatt gttcatatat
     3241 atatctccag ggttaagcct ccacgcttcc catacgaaac ccaccaaggg accctatcgc
     3301 atgtgacgaa acgtgtgtgt atttaaagcg cggtatttat aacatattcg tgctaattca
     3361 gcaatgaaat taacatcgct ctctgtacat gacaaaaata cgtcatgcat aaaagactat
     3421 gtatgaccac agtaattaga caaagcctat taggaaataa gtctatatta atttgtacat
     3481 tttgatttat actgctataa gcatggaagt tttaaaaatg ttgtactcag gagaatccta
     3541 ttaacgataa catttaagag gaaacgataa tgagtaaaaa atctatctgt ttagccacgc
     3601 tcgctttgat atttaccttt ggtcagcctg tctttgccaa ctcaagcgaa gctacacaaa
     3661 caaagccttg tcattgtcat gatcacaaaa aaatactcag tcggcttaat ttaactcaag
     3721 agcaaaaaac caaaatcaaa acaattaaaa tacaagctca aaaatcaatc aaagaaaatt
     3781 atagacaact taaagcgatc aaagaacaaa tcaatgcgct cattgcaaat gagaagcttg
     3841 atgaagccaa gctggatagt ttaatcaacc aaagaaataa aatagcaggg actatgctaa
     3901 aaaatcgtgt aatgatgaaa aatcaaattt acaatcagct aacgaacgaa caaaaatcac
     3961 aatacaaaaa gaatatgtaa aacttgattg gtaccgataa caaaaaattc ggtaccaatc
     4021 tatcccaact cttcagatat agtcattgct gtaattatga tttttctcac tgttgcttcc
     4081 tgaacaatat tttctgagcg caaaaaaatg ggcaaccttt ctctctctac cattaaaaaa
     4141 cagatgatat ttcgcttagt atgctaaccc atagtatgct gcagcagctg cactggcaat
     4201 gatgcaaagt gcaaatatta tagttctcgc catattcttc tttgttttgg gtttcataac
     4261 aaatcctgga atgacggtta tcctgattga tatttcattt aaattatagt tcaaatttta
     4321 tgcatagcta gacccacata tggattgccc ttacacagaa tagcatggag ctttactaca
     4381 aattcagttc aaaacacttg ctataattat tgacttatga accaatttag aacaacctat
     4441 atcagagatg agcaatattg ttgttgcaga ataaaaaaga aacccaatta tgagaggaca
     4501 tatgattttt ttaagtattc ctaaagggat ggaatttaag caaattacag aaaaagacaa
     4561 tacgaacgac tattttgttg atccgaatgg aaagttacca cgaattaata ttcaggcttt
     4621 agttaaagac gctctgcagt acaacaaagg tagaaaaaaa gaaatctccc tgcctgattt
     4681 cactatttat aggcacaaac caccttatag agatgaactc ttccttcagt ataatccaga
     4741 tcataatggc aaatacttta caaaagaatc tgtaaatttg gtgaatggta aagaatttat
     4801 taaatataaa actcctgcta cttcatacgg tactttttgg tttcaaaaag tacaactatc
     4861 cgaaaacaga atggatgaag tccttgccaa gcgaagtgaa caacgagaaa atcgtaggca
     4921 tacaggggat agtccaaacc caacataatc aaacaagaat attgaaaaag gcatgtacaa
     4981 gcatgccttt tttcaaaaag aacaaaatga aattaaatgg caaatgcagt agaagctttg
     5041 gtgtctaatt tttgagtcaa ctgactttgc atgcacaaat atgcgcctac aactttataa
     5101 gactcttgtg aatggaaatt ttttgttgag tattctttag gataagtgag gctttccagc
     5161 cgattatttg ttgcggttat cactttgtct aaatcaatca aggtggtaga gtaattatca
     5221 ccactgtctg ttatcatttt catttcttca agagcctccc gtacaacagt cagaatttta
     5281 ttaaaatttg gaccagcagt ggtgcaacct cgatagatag taattagacg atcgacaaga
     5341 gaaaagaaat taaccatctt ttgcaaattt gactcaagaa tacgcataca tgcaaccatt
     5401 tctgttgtgc tgcgttgagg atatttgtcc attaaagcat tcatcacttc gacaactttt
     5461 ttcacaaacc catctacagc tttaacggaa taactgtttt tcttaaatac ttcaaaaata
     5521 gattgagcat ttctcattgg atcctttaaa gcattcatac ttttaataaa cgcattaatc
     5581 ttctcccaaa aacggctgcc atccttcctg ttttctctag cttcttgata gtttttgatt
     5641 aacattttcg cagtaaaagc atattgggat aagaaaaagc gatcgctttt ctcagccatt
     5701 atcgcctgca aagtaaaaat tggattgttc cattgtcctt catcacacag gacattccat
     5761 tgctgggctt tcgctctgat taaaaagact ttttgttcat ttgataacgt agtgataacg
     5821 ttgatttttg ctttatacaa ttcaaatgca tcaagcttta ttttctctat agcctgttca
     5881 tgagctttaa ttaattcagc gggagaatta aaattattaa caaacttcat tggttgtaac
     5941 ttattgactt caaattcaaa attacaattg tattttgcct gacgaactcc tgcagcatta
     6001 atttcttttc catctatgtc ataaacatct cttgcattct caatagcttt atcaccaaat
     6061 gctttgattg ttttgacata gtcaggatta gtaaagccct ttccctgaac aggaattttg
     6121 taattgtcca tggtatggac attttcacgg ctacaggcat atttaactaa atgcattgta
     6181 acgttagatg gaataagctc tggatttgct gagaaaaatt gatataaacc ttcaagtata
     6241 tcttctcgat catcataaaa aaaataatca atctgctctc tagcttccaa ggaagaagct
     6301 ctttgcatgt gtgcatacac aagcgaagtt ttcgatttat catgtaacca gccgttttga
     6361 atttttgcgc catttgcagg attcgttgcc tcatggaggc tagtacctgc tggcaccaaa
     6421 ttgtatgcat cagctaataa aaacggatca aattccatgc ctaagtcggt gcacagtttt
     6481 tgaatctgga taaaacaaga accattgcca ttggagcgag cattacgctt atcatcgcga
     6541 tcactttgcc gattcgaagc aattagcgcg acttctttag tatagtagtt tgaagtttct
     6601 cgaattcttt ggacaagctt tggattttgt gcaacgatat ttattagatt agtaacaccg
     6661 acgcaaccat cataatcaaa acctgaataa cgaactggca ttttattttc ctctcaaaaa
     6721 gattgcataa ccactgtcac tttaattaag gagaaaattt caaacatgga agtcggaaca
     6781 tcgccttgag taaagtgata ctttcttcat agcgacgcac ccgtttcctc atgaaactag
     6841 aaatagccag tcctctaggc aatccttata gagaagacac atcgaaattt tgctaaacaa
     6901 tatcaagcaa agttcataat tttaacattg tgcaaacata tatgtcaatt tcttatttat
     6961 tgttgttttt ctattcatag ttcaaacagc cccaatcagg ttcaatttgg actgtatcag
     7021 ctctcatata acacgagtta atagagagct cggaatttct gatataagat atgtaaacag
     7081 gaaatatcaa atcaaaaaaa cttgctttcg agtgtgatat ttgaatccat aatacctaat
     7141 tgctctaaca agtc
//
LOCUS       DAXLDT010000149         7136 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXLDT010000149 DAXLDT010000000
VERSION     DAXLDT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.149"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(3..299)
                     /locus_tag="ACF2SP_002506"
     CDS             complement(3..299)
                     /locus_tag="ACF2SP_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360280.1"
                     /translation="MSISSDMTLPIGAALSCLRGAILNLKEAIQSKHSSLIYLRRQEL
                     AEFLNQIRHFDYNSVVNNSIVKLQLHRNLEIAKNQATTILFDASLALSASVHLV"
     gene            527..1588
                     /locus_tag="ACF2SP_002507"
     CDS             527..1588
                     /locus_tag="ACF2SP_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2360281.1"
                     /translation="MASIEKRTNKEGKISYRVKIRLKGFPTQHATFERLTDARKWVQQ
                     TESAIREGRHFKTTEAKRRTLGEMIDRYIKDVIQEKPKNLKNRVLHLNWWKEELGQYS
                     LAELSPAMIAEKRDKLSAGITSRNLKRSPSTVVRYMASLSHAFTIAVKEWGWLEDSPM
                     RRVTKPKEPRGRVRFLSDDERARLLSECKKSDSQYLYLAVVLALSTGGRKMEILGLSW
                     KNVDFNRSIITLHETKNGERRVLPLTGHALDLMKQHAKVRHVNSDLVFPGKDFKTPID
                     LRTPFETALKRAGITDFRWHDLRHSCASYLAMNGASLAEIAEILGHKTLQMVKRYAHL
                     SEAHTSKVVARMNEKIFGV"
     gene            1793..3343
                     /locus_tag="ACF2SP_002508"
     CDS             1793..3343
                     /locus_tag="ACF2SP_002508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360282.1"
                     /translation="MNDQKICLPADLTQRQPNIRFRRPLNALDVLEIKKRFNSILIEL
                     TQKLSKYCDLQEQKTAFSWFEFLEGLQRQAKFDRYIRRYRMVQVESTGEKPRVRPGQQ
                     EAIGSIALIPREFIQYIFYDHYGIEPQIIKDDLIMVNRCTADEAVQKLGIEKHNLIKN
                     CLDDGIGVYVNSSQYQLHRMSGSIQKEIIDARVDYTYLYPYRGLVRLRRSEVERLYDA
                     SITVNGKQYCHVWLQHALAELIQGYAWCIELRGTQQITIDDLWFIDDEISVCLRKSVP
                     VPTEVLGVQTTTSINTTPLTDDRTGSLKTDPQLVFSKKGDLYEITYLDKTIQLSSSIG
                     LDRLAYLVQHPNTKMSSLELAQRCNKSNECSQVNQHYEYNEDLEDEGIGSDAFFSDDP
                     INDSQAQINYKNKETQLINEINALKEKQDDATDKGDLLQAEAFKEQQLEKTSELIQLQ
                     KFTKKDKDNKGKSRRHKTDAEKARSAVQQSIKVAMDKITQKHLELGTYLSNTIETGSQ
                     CLYLLQTS"
     gene            3458..3664
                     /locus_tag="ACF2SP_002509"
     CDS             3458..3664
                     /locus_tag="ACF2SP_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010597306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360283.1"
                     /translation="MTDINKRYLTVSNWNNYHDWPRSGGLRNLIFNRKTNGFDQFNVV
                     KKIGKRVLIDEAAFFAWVDSQGGK"
     gene            3667..3981
                     /locus_tag="ACF2SP_002510"
     CDS             3667..3981
                     /locus_tag="ACF2SP_002510"
                     /inference="COORDINATES: protein motif:HMM:NF025456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360284.1"
                     /translation="MEYKKNRPATQLGKPKSKKPLYGNSASSQRARLLKFFELTPRIS
                     TMEAREMLGILHPGGRIMELRKKGYRIDTNWIEVSDANGVMHRIGLYVYQGKSGGRYE
                     IQ"
     gene            3968..4183
                     /locus_tag="ACF2SP_002511"
     CDS             3968..4183
                     /locus_tag="ACF2SP_002511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360285.1"
                     /translation="MKYNNHADLNDLYHVLRITTESFIDAIIMLSELPPQKIDEGIAS
                     CDRWLEMVRKDFDQFKTDVLGGNYAKK"
     gene            4170..5033
                     /locus_tag="ACF2SP_002512"
     CDS             4170..5033
                     /locus_tag="ACF2SP_002512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360286.1"
                     /translation="MPRNRMIKSEFWTSEQVISCSPMARLLFIGLWNFADDNGVHPAS
                     FVKIKAEVFPGDNIDISEIKKWITELIQQCLLIEYVINEKTYWIVTGWKIHQKIDKPT
                     YRYPMPYSDLKLIPISPEPDSTSERQKVVEDFTTIHRDINDLSAKSSDEIEKKKKRKK
                     KEKYICEVKTSPAEVSKITYSASKEEVFRYWQETMNHPKAKLDKKRSSVIHKALQLGY
                     SVSDLKQAIIGCAQTPFNMGQNDRQQIYDDISLILRDAEHIERFINNATNPLNEKQIY
                     NTNNNLMSGVL"
     gene            5035..6867
                     /locus_tag="ACF2SP_002513"
     CDS             5035..6867
                     /locus_tag="ACF2SP_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFD2360287.1"
                     /translation="MMMAKEISRQLALQAEDVARYLLPNGKRQGSEWSVGSINGEAGE
                     SLKVHIIGEKAGVWCDFATGDKGDLLDLWAAKHNLKISEAIKEVSLYLGISIPKFEGH
                     KPLKFVRPVLKNLSPLLQKTSPIMSYLINERRLTAETIEAYKIGSQNSKIIFPYWRDG
                     ELIFVKQLDLERVNGKKQIAVEPNCEPCLFGWHLIPANARKVTICEGEIDAMSLYQYG
                     FPALSVPFGGGGGNKQKWLEYEFERLAVFDEIYLCFDNDKEGDIATLELVERLGRHRC
                     RIVKLPCKDANECLQAKITQEVIAQCFHTASMLDPEELKPAHQFVEQVIDVFYPPVGS
                     HQGYNPPWEKTKGKILFRPTELSLWTGINGHGKSQFLGQVVLHAMKEGARVCIASLEI
                     MPKRLLMRLTRQAGALESPSIEYIHAIHSWYEDKLWIFDLVGNAKSKRILDVFLYARQ
                     RYGINVFVIDSFMKLDIAEDDYKAQKNFMEQLCDFKNQHNCHIHIIVHPRKGTDESQP
                     PGKLDNKGTGAISDLADNCFTIWRNKHREFLKQKQSIGEPLEPKELKKLNESDCLWCC
                     DKQRNGEWEGRFGFWFHRESLQYLGKPDQKPVRVVDYSINTTKV"
     gene            complement(6864..7124)
                     /locus_tag="ACF2SP_002514"
     CDS             complement(6864..7124)
                     /locus_tag="ACF2SP_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2360288.1"
                     /translation="MIRKHVNLIKIGDQIRTIRSSKGFSQEGLAAAATLGRTYMGRVE
                     RGEQNISIQNLIKIAFILNVNVGELVPPLCELENPANIRSND"
BASE COUNT         2420 a         1212 c         1461 g         2043 t
ORIGIN      
        1 gcttatacca gatgaacaga tgcagataaa gccaaagaag cgtcaaacaa aatcgtagta
       61 gcttgatttt ttgctatttc taaattgcgg tgtaattgaa gtttaacaat agaattgttt
      121 actacactat tgtaatcaaa atgtcttatt tgatttaaaa attcagccag ttcttggcgc
      181 cttaaataaa tgagagagct gtgctttgat tgtattgctt ctttaagatt taaaatagca
      241 ccccttaagc atgataaagc tgccccaata ggtaaagtca tatcactact tatagacatt
      301 tatgaattat atggtatgga aaagaatatt tgataatatt ttacccaata aatcaagagt
      361 tttttacatt agtgtcccat gaaagccctt tattcgaatt aaatttaaat aaatataata
      421 aaaacataat attaataaat taaaatataa gattatttat cctatgttgt cattgattta
      481 agtttattgt actctttcgc tattaataat tatgtgtggc tatttgatgg caagtataga
      541 gaaacgcact aacaaagaag gaaaaattag ttatcgagtc aaaatcagac taaaaggttt
      601 tcctactcaa catgcaactt tcgagaggtt aacagatgcc cggaaatggg tgcagcaaac
      661 tgaatcagca attcgagaag gtcgccactt taaaacaaca gaagctaaac gtagaactct
      721 tggtgagatg attgataggt atatcaagga tgttatccag gaaaaaccta aaaatcttaa
      781 aaatagagtt ttacatctta attggtggaa agaagaactt ggtcaatata gtttagccga
      841 attatcacca gcaatgatcg cagaaaaacg cgacaagcta tcagctggga ttactagtcg
      901 taatttaaag cggtcgccat ctacagtggt acgttacatg gcttcccttt ctcatgcttt
      961 tactatagca gtgaaggagt gggggtggtt ggaagattct ccaatgcgaa gagttacaaa
     1021 gccaaaagaa cctcggggcc gtgttcgttt tttatctgat gatgaacgag cacgtttatt
     1081 atctgaatgt aaaaaaagtg acagccaata tttatacctc gctgttgttc ttgcgctttc
     1141 aacaggcgga cgaaaaatgg agattctggg actaagctgg aagaatgtcg attttaatcg
     1201 cagcattatc acattacatg agacaaaaaa tggtgaacgt agagttctgc cattgactgg
     1261 acatgctctg gatttaatga aacaacatgc taaagttcgt catgtaaata gtgatttagt
     1321 ttttccggga aaggatttca agacaccaat tgatttacgt acaccctttg aaacggcttt
     1381 aaagcgagct ggaataacag attttagatg gcatgatcta cgccatagtt gtgcctcata
     1441 tctcgctatg aacggagcca gtttagcaga aattgctgaa atccttggac ataagaccct
     1501 acaaatggtg aagcgatatg cgcatttgtc tgaggctcat accagtaagg tagtagcgcg
     1561 gatgaatgag aaaatatttg gggtataacc gatgtggaat tgtaatctga gtacagaatg
     1621 gataaataat acactagatc aattggctaa agatcctcaa ttattgtata gccatgatat
     1681 ttcattgttt gattcttggg taacattcgt tcaatcaagg attgtaaaaa aagaagagtt
     1741 taaagcatct gtatttttac tggcttgcat tggatatttt gttttttatg atttgaacga
     1801 tcaaaaaata tgcctaccag cagatttaac tcaacgccaa cctaacatta gatttaggcg
     1861 gccattaaat gctttggatg tcttagaaat aaaaaagcga tttaatagca ttttgatcga
     1921 attaacccaa aaattatcta aatattgtga tcttcaggaa cagaagaccg cattttcttg
     1981 gtttgagttc cttgagggat tgcagcggca agccaagttt gatcgttata ttcgaagata
     2041 ccgaatggtc caggtggaat cgacaggaga aaaacctagg gtacgccctg gacaacaaga
     2101 agcgatcgga tcaatagccc ttattccacg ggaatttatt caatatattt tttatgacca
     2161 ttatggtatt gaacctcaaa taatcaaaga cgatttgata atggtaaacc gatgtacagc
     2221 agatgaagca gtgcaaaaat taggtattga aaagcataat ctgatcaaaa attgtctgga
     2281 tgatggcatt ggtgtatatg ttaatagcag tcagtatcaa ttacatcgta tgtcaggctc
     2341 tatacaaaaa gagatcatag atgccagagt agattacaca tatctttatc catatcgtgg
     2401 gcttgtgcgt ttacggcgat ctgaagtaga aagactgtat gatgcctcca tcacagttaa
     2461 tgggaaacaa tactgtcatg tatggctgca acatgctttg gcagaactaa ttcagggcta
     2521 cgcttggtgt atagagctgc gaggaacaca acaaataacc atagatgatt tatggtttat
     2581 tgatgatgaa atatcagtgt gcttaaggaa atcggtacca gttccaacgg aggttcttgg
     2641 ggtacaaaca actacctcaa taaacaccac tccccttact gatgatcgga ctggttcttt
     2701 gaaaactgat cctcagctag ttttttctaa aaaaggcgat ttatatgaaa tcacttatct
     2761 agataaaacc atccaactct cttcttccat tggcttagac cgattggcgt accttgtgca
     2821 gcaccccaat acaaaaatga gctcattaga attagctcaa agatgcaaca aaagcaatga
     2881 atgtagtcaa gttaatcaac actacgaata taatgaggat ttagaagacg agggaattgg
     2941 tagtgatgca tttttcagtg atgatcctat aaatgatagt caagcacaaa taaattataa
     3001 aaataaagaa acacagctta taaatgaaat taatgccctc aaagagaaac aagatgatgc
     3061 gacagacaaa ggtgatttat tacaggctga ggcatttaaa gaacagcaac tagagaaaac
     3121 aagcgagcta atacaacttc agaagtttac gaaaaaggat aaagataata aaggaaaatc
     3181 taggcgccat aaaacagacg cagagaaagc aagatctgct gttcagcaat ccattaaagt
     3241 tgccatggac aaaataactc aaaaacatct tgagttaggg acttacttaa gcaatacaat
     3301 agaaacaggc tcgcagtgcc tatatctact acaaacatcc taatatctta caaatcgtaa
     3361 gatttaattt tacgatttgt aagttcaaaa cttacgcatt acctcttgta gcacacagca
     3421 atttcgcata gtgcttataa acaacaagag gtcaataatg actgatatta ataaacgtta
     3481 tcttactgtc tcaaattgga acaactatca cgactggcca cgcagtggtg gcttacgtaa
     3541 tttaatcttc aatcgaaaaa cgaacgggtt tgatcaattt aatgtggtta aaaaaattgg
     3601 caaacgagtg ttaatcgatg aagctgcttt ttttgcttgg gttgatagcc agggagggaa
     3661 atagcaatgg aatataaaaa gaaccgccca gccacacagc tgggcaagcc taaatccaaa
     3721 aagcctttat atggtaattc agcttcttca caaagagcaa gacttttgaa gttttttgaa
     3781 ctgactccca ggatttcaac catggaagct cgtgagatgt taggcatatt acatcctgga
     3841 ggtcggatta tggagctgcg taaaaaaggt tatcgcatcg atactaactg gatagaagtg
     3901 tctgatgcaa atggagtcat gcacagaatt ggtttgtatg tgtaccaagg caagtcagga
     3961 ggtcggtatg aaatacaata accacgctga tctcaacgat ctttatcacg ttcttcgaat
     4021 taccactgag agtttcattg atgcaatcat catgctctcg gaacttccac cacaaaaaat
     4081 tgatgaaggg atcgcctctt gcgatagatg gttggagatg gtgagaaagg actttgatca
     4141 gttcaagaca gatgttcttg gaggtaacta tgccaagaaa tagaatgatt aaaagcgaat
     4201 tttggacaag tgagcaggtt atatcttgct cacctatggc cagattactt tttattgggc
     4261 tctggaactt tgccgatgac aacggcgtac atcccgcatc ttttgtgaaa attaaagctg
     4321 aagtttttcc tggggataat attgatatta gtgagatcaa aaaatggatt actgaactca
     4381 ttcaacaatg cttgcttata gaatacgtca ttaatgaaaa aacatattgg attgtcactg
     4441 gttggaaaat tcatcaaaaa attgataaac ccacttatcg ttatccaatg ccctactctg
     4501 atttaaaact tattcccata tcacctgagc cagactcaac aagtgaacgt caaaaagtag
     4561 tcgaagattt tactactatt catcgagata taaacgacct ttcagctaaa agtagcgacg
     4621 aaatagaaaa gaaaaaaaag agaaagaaaa aagaaaaata catatgtgaa gttaaaactt
     4681 cacctgctga ggtttctaaa ataacttatt ctgccagcaa agaagaagta tttcgctact
     4741 ggcaagaaac aatgaatcat cccaaggcca agctcgataa aaaacgttcc agtgttattc
     4801 ataaggcttt gcagttgggt tatagcgtta gtgacttaaa gcaagcgatt attggttgtg
     4861 cacaaactcc atttaatatg ggacagaatg accggcaaca gatttatgat gatatcagtt
     4921 taattttgcg tgatgcagag catatcgagc gttttataaa taatgctacc aatcctctca
     4981 atgaaaaaca aatttataat acgaataaca atttaatgtc gggagttctt taacatgatg
     5041 atggctaaag aaatatctcg gcaacttgct cttcaagccg aagacgtggc tcgatattta
     5101 ttaccaaatg ggaaacgaca aggcagtgaa tggagcgttg gtagtattaa tggagaagct
     5161 ggcgagtctt taaaggtaca tattattggt gaaaaagcag gagtatggtg tgatttcgct
     5221 acgggcgata aaggtgatct acttgatttg tgggcagcaa aacacaactt aaagatctct
     5281 gaagcaatta aagaggtgag cctatacctt ggtatatcaa tacccaaatt tgaggggcat
     5341 aagcccttaa aattcgttag acctgtattg aaaaatctat ctccgttact ccagaaaacc
     5401 tcgcctatca tgagctattt aattaatgaa agacgactga cagctgaaac aatagaagct
     5461 tataaaattg gtagtcagaa tagcaagatc atttttcctt attggcgcga tggtgagctc
     5521 atttttgtga aacagcttga tttagagcgc gttaatggaa aaaaacaaat agcagttgaa
     5581 ccaaattgtg agccttgctt atttggctgg catttaatac cagccaatgc acgcaaggta
     5641 acgatttgtg agggtgaaat tgatgcgatg agtttgtatc aatacgggtt tccggcactt
     5701 tcagtccctt ttggtggggg tggtggtaat aaacaaaaat ggcttgagta tgaatttgag
     5761 cggttggcag tttttgatga gatctaccta tgttttgata atgataaaga aggagatata
     5821 gctactttag aattagttga gcgattaggc cgtcatcgtt gccgtattgt taaattacca
     5881 tgcaaagatg caaatgagtg tttgcaagcg aaaatcacac aagaagtaat agcgcaatgc
     5941 tttcatactg cgtctatgct tgatccagag gaattaaagc cagctcacca atttgtagaa
     6001 caggtaattg atgtgttcta cccaccagtt ggaagccatc aaggctataa cccgccttgg
     6061 gaaaaaacaa agggtaagat tttgtttcga cccactgagt tatcactgtg gacgggcata
     6121 aatggtcacg gtaagagcca atttctcgga caagtggtac ttcatgctat gaaggaaggg
     6181 gctcgtgttt gtattgcaag ccttgaaatt atgcctaaga gactacttat gcgcctgaca
     6241 cgccaagcgg gtgcattaga aagccccagt atcgaatata ttcatgcgat ccatagttgg
     6301 tatgaggata aactgtggat ttttgatcta gtaggcaatg ccaaatctaa acgaatatta
     6361 gacgtttttc tttatgctcg ccaacggtat ggcattaatg tgtttgtgat tgattcgttc
     6421 atgaagctcg acattgctga agatgattat aaagcccaga aaaactttat ggagcaatta
     6481 tgcgatttta aaaatcaaca taattgtcat attcatatta ttgtccatcc acgcaaagga
     6541 actgatgaat cgcaacctcc tgggaagcta gataataaag gcacaggtgc aatcagtgat
     6601 ttagcagata attgttttac tatatggcgg aataaacaca gggaatttct caaacaaaaa
     6661 caatcaatag gtgaaccact ggaaccaaaa gaattaaaaa aactaaatga atcagactgc
     6721 ctttggtgtt gcgataaaca acgtaatgga gaatgggaag gaagatttgg attttggttt
     6781 catcgggaat cattgcagta tctgggcaaa ccggatcaaa agcctgttcg agtagtggat
     6841 tattcgataa atacaactaa ggtttaatcg tttgaacgaa tgttggcggg gttttcaagt
     6901 tcacatagag ggggaacaag ctcccccaca ttaacattaa gtataaaagc tatttttatc
     6961 aggttctgta tagagatatt ttgctcacca cgttcaactc gtcccatata cgtccgtcct
     7021 aatgtagcag cagcagccaa cccttcctga gaaaatcctt tggaagatct tatcgttcga
     7081 atttgatcgc ctatttttat aaggtttacg tgctttctta tcattcaata agtaaa
//
LOCUS       DAXLDT010000150         7054 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXLDT010000150 DAXLDT010000000
VERSION     DAXLDT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.150"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(451..1335)
                     /locus_tag="ACF2SP_002515"
     CDS             complement(451..1335)
                     /locus_tag="ACF2SP_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFD2360289.1"
                     /translation="MISDHFICKKAKIIAMSSLFITTVAFAAPSIQYASPMGEENWRM
                     TGNPLRCGLSLTIPNYGIGYFEQYATQSPHFILRKWDQVQRALPAQVVASPPVWKPLL
                     KGSYLVTRTYIKPGEYGLFLNRQPALKLLTFLSQGYQANFSYLSEEGFRTTVILSPVR
                     FQKVYSQYQRCLGSLLPFNYEDVKESVFHFGVDSKLLSDADKAQLQRIAQYVDADVQI
                     DVIKIIGHADDSGRKGYNNAISQYRAESVKNYLIRLGVPQRKLSITWVGALQPIARND
                     TDEGRAANRRVVVTLIKK"
     gene            1405..2442
                     /gene="pyrC"
                     /locus_tag="ACF2SP_002516"
     CDS             1405..2442
                     /gene="pyrC"
                     /locus_tag="ACF2SP_002516"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFD2360290.1"
                     /translation="MQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKP
                     ALTNLAAIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYP
                     AGATTNSEEGAKSLKALYPLFETLQTSNLVLQIHGEVTHSDIFEREALFIDEYLKPIA
                     KHFPKLRIVLEHISTKAAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLP
                     ILKHEKDQKALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPFAVALYTQI
                     FDELNQLKKLNDFLSRFGAEFYQLPINQQQLELIKAPQTIPNSMPFGSTHVVPIAAGD
                     TINWSINGFSQ"
     gene            2426..3049
                     /gene="rnt"
                     /locus_tag="ACF2SP_002517"
     CDS             2426..3049
                     /gene="rnt"
                     /locus_tag="ACF2SP_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFD2360291.1"
                     /translation="MASHNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIV
                     LLDMDEQGLLKKESSYFEHILPFPGAELDQKSLAFNQIDPYQPLRFAVDEQTALERLF
                     KPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
                     GETVLAKAVQAAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI"
     gene            complement(3175..3780)
                     /locus_tag="ACF2SP_002518"
     CDS             complement(3175..3780)
                     /locus_tag="ACF2SP_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFD2360292.1"
                     /translation="MSVLVGRKAPDFTVAAVMGNGEIVDKFNLHEHLKGKYGLVFFYP
                     LDFTFVCPSELIALDHRIEEFKRRNVEVVAVSIDSHFTHNAYRNTPVKNGGIGPVRFT
                     LAADMTHSICQSYGVEHPVAGVAFRGAFVIDTNGMVRSQIVNDLPIGRNIDEIIRIID
                     AVQFFEENGEVCPAGWQKGQAGMKASPKGVAEYLSEHSESL"
     gene            complement(3833..4150)
                     /gene="grxD"
                     /locus_tag="ACF2SP_002519"
     CDS             complement(3833..4150)
                     /gene="grxD"
                     /locus_tag="ACF2SP_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFD2360293.1"
                     /translation="MDTLEKIKKQIAENAIMLYMKGTPKMPQCGFSARAVQCIEACGV
                     DFAYVDILANPDIRQVLPQFSDWPTFPQLYVKGELIGGSDIIAEMFQQGELEPMLRDA
                     VAA"
     gene            4481..5059
                     /gene="sodB"
                     /locus_tag="ACF2SP_002520"
     CDS             4481..5059
                     /gene="sodB"
                     /locus_tag="ACF2SP_002520"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFD2360294.1"
                     /translation="MTFTLPQLPYALDALAPHVSKETLEYHYGKHHNTYVTNLNKLIP
                     GTEFESMTLEEIIMKAKGGIFNNAAQVWNHTFYWHSMSPNGGGEPKGRLAEAINKSFG
                     SFAAFKEQFSQTAATTFGSGWAWLVQDQSGALKIISTSNAGTPMTEGLNALLTCDVWE
                     HAYYIDYRNRRPDYIEAFWSLVNWDFASSNLK"
     gene            5119..6288
                     /locus_tag="ACF2SP_002521"
     CDS             5119..6288
                     /locus_tag="ACF2SP_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFD2360295.1"
                     /translation="MSLITSYSPMPVTFTHGEGVWLYDEQGKKYLDGLSGIAVCGLGH
                     AHPDVTRTIQEQAARLIHTSNVFHIKEQELLAAKLTSMARMEQVFFANSGAEANEAAI
                     KLTRLFGHKKGIETPSIIVMEKAFHGRTMATLTASGSRKVQAGFEPLVPGFIRAPFND
                     LDAIHTIAANREDVVAVMVEPIQGEGGIYPAEEGYLRALANLCEQNDWMLILDEIQTG
                     NGRTGKLYSCMHYDIQPDILTTAKGLGNGMPIGACLMSKRAKDLFKPGNHGSTFGGNP
                     LACATALTVLEVIERDKLCEHVTKNSALLMDKLISNLGEHPHVKAIRGKGYMIGIELD
                     RPANDMRVLGLENQVLFNITAETVVRLLPPLIISEDEIDELVNRITRTINQFTNQ"
     gene            complement(6417..>7054)
                     /locus_tag="ACF2SP_002522"
     CDS             complement(6417..>7054)
                     /locus_tag="ACF2SP_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD2360296.1"
                     /translation="SLRDQGLSYKDIKNIYISHLHYDHCGGLEWFALITYFDPNLSHK
                     PCLIASDKIIKELWDKSLSGGLSTLPHERANLSAFFDVKAVKQYEGFVWQSIKFKLLQ
                     TVHYYSEYELMPSFGLIFSYNKSRILFTSDTQSCPVQLISFYEESDIIFHDCETTESK
                     SGVHAHYSELVKLPSHLKKKMWLYHYNPGKLPNAKKDGFLGFVAKGQSFVF"
BASE COUNT         2202 a         1472 c         1314 g         2066 t
ORIGIN      
        1 gtgataccaa tatcccatgc agttctttcg caaggaacag ttacattacc tggggtgcaa
       61 actggtccca tggttccagc aaacactgca ccgcttccta aggcgagtac tgccactgct
      121 gtttttttca aactaaacat gccttgtctc cacttgttta ttattgagtt tccattttat
      181 tgataactca ccatttggag ttgtcaatag cacgcatcgt cgcttaggtt cgaagcatgc
      241 aacgatatga gtatctgcag tgattttaaa taagtcaaga aaatttatta aaaattccaa
      301 tattttcatg acgatataac taaatttgaa cagtatctct atttttttaa cattattacc
      361 atgtctcttt caaaagaaat ttgctgacca agaagggggt tttcgagaat gaaataccat
      421 catgaaagat acttttttta atcacaaaat ttattttttt atcaatgtca caacaacacg
      481 tctgttagca gctctcccct cgtcagtatc attcctggct atcggttgca aagctcctac
      541 ccaggttatt gatagttttc tttgaggtac tccaagccta atcaaataat ttttcacaga
      601 ctctgctcga tattgtgaaa tggcattatt gtaaccttta cggccactat cgtcagcatg
      661 accaataatc ttaatgacat caatttgtac atcagcatcc acgtattggg caatccgttg
      721 taattgcgct ttatcagcat cagacaataa cttactatcc accccaaaat gaaacacgct
      781 ttcttttaca tcctcatagt tgaagggcaa cagactacca agacaacgtt gatattgcga
      841 gtacactttt tgaaaccgaa caggtgagag tataaccgta gtgcgaaatc cttcctctga
      901 taaatagcta aaatttgcct gatatccttg cgataaaaat gtcaataatt ttaaagcggg
      961 ctgtcgattt aaaaatagtc catactcccc gggtttaata taagtgcggg ttaccagata
     1021 ggaccctttc aataatggct tccacactgg gggacttgct acaacttgag caggtaaggc
     1081 ccgttgaact tgatcccatt tacgcagaat gaagtgaggg gattgagtag cgtattgttc
     1141 aaagtagcca atcccataat ttggaatcgt caatgacaaa ccacatcgaa gaggattgcc
     1201 tgtcatacgc caattttctt ctcccatagg gcttgcatat tggatagagg gagcagcaaa
     1261 ggcgacagta gtaatgaata aagaactcat cgcaataatt tttgctttct tgcatataaa
     1321 atgatctgaa atcatttttt gctcatccca attgcccaat tcatgttatt ctcttttatc
     1381 ggcaataatg caggaacctt tattatgcaa actctgataa ttaatcgccc tgacgattgg
     1441 catctccatc ttcgggatgg agagtttcta actcataccg tccaggcgtc tgcccaacat
     1501 tttgccagag cgttagtgat gcccaatctc aaaccagcac taactaattt agccgcaatt
     1561 gagagttata gaaacagaat catttcggct attcccaaac aaaactcctt tattccttat
     1621 atgacttttt atttaaatga gtccgttaaa ccagaagaat tacagcaagc cgcctctctt
     1681 tcttatatag tgggagcaaa actttatccg gctggagcga cgacaaattc tgaagagggt
     1741 gctaaatctt tgaaagcact ctaccctctt tttgaaacat tgcaaacgag taatttagtc
     1801 ttgcaaattc atggcgaagt cactcatagt gatattttcg aacgcgaagc actatttatt
     1861 gatgaatacc tgaaacccat tgctaaacat ttccccaaat taagaatcgt tctggagcac
     1921 atatcaacca aagctgccgt ggattatgtt tcagaaggac ctgctaacct tacagcaaca
     1981 ataactcctc accatttgct ttataatcgc aataagctac tcgcaggggg cattagacct
     2041 cattactatt gtttgcctat tttaaaacac gaaaaagacc aaaaggcctt acaaattgca
     2101 gcgacgagtg gaaatccaaa attttttgcc ggcactgaca gtgcccctca tgccatcaat
     2161 gccaaggaaa atgcatgtgg atgcgccggt atttactcag ctccatttgc ggtagcgctc
     2221 tacacgcaga tctttgatga acttaaccaa ttaaaaaaac ttaatgattt tcttagtcga
     2281 tttggcgcag agttttacca actccctatt aatcaacagc aattggaact cattaaagca
     2341 cctcaaacta tccccaactc aatgccattt ggatcaactc atgttgtgcc tattgctgct
     2401 ggagacacta taaactggag tattaatggc ttctcacaat aatattctaa gtaacaccat
     2461 taaaaatcgc tttcgtggct ttttacccgt tgttgttgat gtagaaactg cgggaataga
     2521 ccctcaaaaa aatgctctcc tggaaatgtg tattgtttta ttggatatgg atgagcaagg
     2581 cttgttaaaa aaggagagta gttattttga acatatttta cctttcccgg gtgcagaact
     2641 ggatcaaaaa tcactggcat tcaaccaaat agacccgtac cagcctcttc gttttgcagt
     2701 agatgaacaa actgcccttg aacgattatt caaacccatc catgacttgc taaaaaaaac
     2761 ccgttgccaa agagctgttt tagtaggaca taatgcctgg tttgatttgt tattcatcaa
     2821 agcggcaatc aaaagaaccg ggataaaatc accattccat gccttcactt gctttgatac
     2881 agcaacatta gcgggtttga tttttggaga aaccgtattg gccaaagcag tgcaagcagc
     2941 tggattacat tttgacagcc aggaagcgca ctctgccatt tatgatgccg aaaaaacagc
     3001 tgatttgttt tgccttatga taaacaagtg gcgaatgcta aacatctgat atcagaaaaa
     3061 cataaatagc ctggattacc taaaactgta atccaggagt ttgatagaaa ttttcttgtg
     3121 ttatgcttct caataaaaca caacaacaaa aagaccccat ttccttgtct tatattacaa
     3181 ggactcggag tgctcagaca ggtattctgc aacaccttta ggagacgctt tcataccagc
     3241 ttgtcctttt tgccagccag caggacacac ttcaccattt tcttcaaaga attgaactgc
     3301 atctatgatg cgaataatct catcaatatt tcttcctatc ggcaagtcat tgacgatttg
     3361 cgaacgaacc ataccattcg tatcgataac aaaagcgcct cggaaagcca caccagcaac
     3421 tggatgttct acaccatagg attggcaaat actatgagtc atgtcagctg ccaaagtaaa
     3481 tctcactggc cctattccac catttttcac aggcgtattc ctatacgcat tatgagtaaa
     3541 atgtgaatca atagatacag caaccacttc aacattacgt cttttgaact cttcgatacg
     3601 atgatcaaga gcaattaact cggaaggaca tacaaaagta aaatccaggg gataaaagaa
     3661 aactaatccg tatttgcctt ttaaatgttc gtgtaaattg aatttatcaa caatttcccc
     3721 attgcccatt actgcagcca cagtaaaatc aggtgcttta cgcccaacta agacactcat
     3781 tctattatct ccattagcgg tgggtgagta aatcccacaa ttgttattaa aattatgccg
     3841 ctactgcatc acgcaacata ggctctaact ctccttgctg aaacatttcg gctatgatgt
     3901 cagatcctcc aattaattcc cctttcacat aaagttgagg aaaggtaggc cagtcagaaa
     3961 attggggtaa aacttgacga atatcaggat tagcaaggat atcgacataa gcaaagtcta
     4021 ctccacaagc ctcaatacac tgaacggcac gagcggaaaa cccgcattgc ggcatctttg
     4081 gagtgccttt catgtagagc attatcgcat tttcagcaat ttgcttttta attttttcta
     4141 aagtatccac tatgcacctt ttataaaata aaaaacccaa ttatgtcgaa aaccaaacaa
     4201 ggtcgcaact aatgattcta ttgtgataat cctatcaatc tttttcttat agtgatattt
     4261 taccctacct cagcgatcaa taaaatacca atttagtact aaatgtgcca aaaaccccca
     4321 ccttgacgag aattaaaatc cactattttg taaaaaatta ttataccagc agaaatgtat
     4381 ccatggccaa gactataaaa atattgaatc gctcagtaat ggcacattga atatcacact
     4441 tatcctgata aactaaagac ttttaatcct ggagattgat atgactttta ccctgccgca
     4501 attaccttat gcattggatg cactggcccc tcatgtttcc aaagaaacct tggaatacca
     4561 ttatggtaaa caccataaca cttacgttac caacttaaat aaattaattc ccggaacaga
     4621 atttgaatcc atgactctgg aagagatcat catgaaagct aaaggcggta tttttaacaa
     4681 tgcagctcag gtttggaatc acacctttta ttggcacagc atgagcccta atggtggagg
     4741 tgagccaaaa ggcaggctgg cggaagccat taataaaagc tttggctctt ttgcagcatt
     4801 caaagaacaa ttttcacaaa cagctgctac aacattcggt tccgggtggg catggctagt
     4861 ccaggatcag tcgggggctt taaaaattat cagcacaagc aatgctggaa caccaatgac
     4921 tgaaggatta aatgccttat taacttgtga cgtatgggag cacgcctatt acatcgatta
     4981 tagaaatagg cgcccggatt atattgaagc cttttggtca ttagttaact gggattttgc
     5041 gagcagtaat ctgaaataaa ataacaagct ggatttgttg caatccttac cggataattt
     5101 tatgtttatg gagacatcat gagtttaatt acaagttaca gcccaatgcc tgtaaccttc
     5161 acgcacggag aaggcgtgtg gttgtatgat gagcagggga aaaaatacct cgatggctta
     5221 agcgggatag cagtttgtgg attaggtcat gcgcaccctg atgtcacccg caccatacag
     5281 gagcaggcag ccaggctaat acatacttct aatgtctttc atattaaaga acaagaatta
     5341 ttggccgcca aactcacttc gatggcacga atggagcagg ttttttttgc taattctgga
     5401 gcagaagcta atgaggcagc catcaaactt actcgtctgt ttggtcataa aaaaggaatt
     5461 gaaacgcctt ctattattgt aatggaaaaa gcatttcatg gtcgcactat ggccacctta
     5521 actgcatcag gcagtcgtaa agttcaagct ggttttgaac ccttggtccc tgggtttatt
     5581 cgtgctcctt tcaatgatct tgatgccatt catactattg cagccaacag agaggatgtg
     5641 gttgcagtta tggtagaacc gattcagggc gaggggggaa tttatcccgc agaggaaggt
     5701 tatctgcgtg cattggctaa cctttgcgag cagaatgact ggatgttaat tttggatgaa
     5761 atacaaacag gaaatggcag aacaggtaag ctttattctt gtatgcatta cgacatacaa
     5821 cctgacattc ttactacggc aaaaggactt ggaaacggta tgccaattgg ggcttgttta
     5881 atgagcaagc gagctaaaga tttatttaaa cctggaaatc atggctcgac ttttggtggc
     5941 aaccctttag cttgtgcaac ggctttgact gttctggagg tcattgaacg agataagcta
     6001 tgtgagcacg tcactaaaaa cagtgctttg ttaatggata aattaattag caacctggga
     6061 gaacatcccc atgtaaaagc catacgtggt aaaggttaca tgataggaat agagttggac
     6121 aggcctgcaa atgacatgcg cgtcctgggt ttggaaaacc aagtcttgtt caatattaca
     6181 gctgaaacag tagtaagatt attacctcca cttattatca gtgaagatga aattgacgaa
     6241 ttggtcaatc gaataactcg tactattaac cagttcacca atcagtagtg agaagacaac
     6301 tttctctcag ttgaatagaa attgtttgct ccctggctgt ctcgcagtat gctaaattaa
     6361 tctgatatct gacagcatgc tccctggatt atccgggagc atgtgtgtga gttacatcaa
     6421 aacacaaagc tttgcccctt ggcaacaaaa cctaagaaac catccttttt ggcattaggc
     6481 agtttccctg gattataatg atatagccac atttttttct ttaaatgaga gggaagttta
     6541 acgagctctg agtagtgcgc gtgtaccccg cttttagatt ctgtagtttc acaatcatga
     6601 aagataatat ctgattcttc atagaaggaa attaactgta ccggacaact ttgtgtgtct
     6661 gatgtaaaaa ggatgcgtga tttattatag ctaaatatca aaccaaaact tggcatcaat
     6721 tcatattcgc tatagtaatg gactgtttgt aacaatttaa actttattga ttgccaaaca
     6781 aaaccttcat attgttttac cgccttaaca tcaaaaaaag cgctaagatt tgctctctca
     6841 tgaggtaaag tgcttaaccc gccggataaa cttttatccc ataattcctt aataatttta
     6901 tctgaagcga ttaaacaagg tttgtgcgag agattaggat caaaataggt tattagagcg
     6961 aaccattcca atccgccaca atgatcataa tgcaagtggc taatataaat attttttata
     7021 tctttataac tcaatccttg atctcgtaat gagt
//
LOCUS       DAXLDT010000151         6960 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXLDT010000151 DAXLDT010000000
VERSION     DAXLDT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.151"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(190..693)
                     /locus_tag="ACF2SP_002523"
     CDS             complement(190..693)
                     /locus_tag="ACF2SP_002523"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFD2360297.1"
                     /translation="MESNNTQQSIKTHSSVIVLYDLSKDSLILTKRSQMLNKHPGEVC
                     FPGGFQEINDQDLYSTALRELNEELGVTSDRITLIRKLNIEKTLLGLVIHPWYASIDS
                     IFPYTMNPQEVSKLILVPMPLVKNQKNYKEIRIEKEGKHFVTCQFIANEELIWGATAR
                     IMRQLAS"
     gene            complement(680..2002)
                     /gene="pabB"
                     /locus_tag="ACF2SP_002524"
     CDS             complement(680..2002)
                     /gene="pabB"
                     /locus_tag="ACF2SP_002524"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFD2360298.1"
                     /translation="MIDSTLITLDYHDFSSESYEKFSRLPGFVLLQSTDKSMGRYDIL
                     SAYPYERMVIKNNIHDKNLVLTELKNKLKIEQSELDLPFQGGAIGYVSYDFGTLLHNH
                     EQYPQKDLAHIPVLNFGFYDWAIIVDHHLKKVTLFARNSHSSTKSVVDEIKNILNNQP
                     AKSVQFSVQAEFLPLISKENYRSSFNAIQQALRAGRSYQVNFTQPFQSKFTGDPWYMY
                     QRVNRNNFVPYAAFIRTEEADILSFSPERFLLYDNGEMLTSPIKGTIHRSKDPVVDKK
                     LKEQLLKCPKNKAENVMIVDLLRNDLGKLAKAGSVKVMNLCHIQSFNAVHHLVSDIRA
                     LCKEDIHPIEAFFSCFPGGSITGAPKLETMKIISEQEIYKRGVYCGCIAYFSQHGRFD
                     ANIAIRTVTAKDNVLYLAAGGGIVIDSDWEDEYRECFTKITAIINGIK"
     gene            2183..3256
                     /gene="pdhA"
                     /locus_tag="ACF2SP_002525"
     CDS             2183..3256
                     /gene="pdhA"
                     /locus_tag="ACF2SP_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFD2360299.1"
                     /translation="MTTVAQFEITYTQYLNEQGKLVAELPPFADNQSILKELYKIMVL
                     TRTFDKKAIALQRTGKMGTYAPINGQEAISTAIGHALKPEDVLVPYYRDYAAQFQRGV
                     KMSEILAFWGGDERGSHYSNNAEDLPICVPIASQCLHAAGVAFAFKYRNQPRVAVVCI
                     GEGGTSEGDFYEAMNVAGAWKLPVVFVVNNNKWAISVPIEKQTGSQTIAQKAIAAGFS
                     GVQIDGNDIFAARQVIGDAIEKARQGGGPTLIEALTYRLCDHTTADDATRYQPSHEVE
                     EAKAKEPIVRFKHYLMQQNIWTSQDEEKLVIECSETVEKAVEEYLNTKPQPVSSIFDY
                     HYAELPEYLIEQRAIAMEEYSHA"
     gene            3249..4223
                     /locus_tag="ACF2SP_002526"
     CDS             3249..4223
                     /locus_tag="ACF2SP_002526"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFD2360300.1"
                     /translation="MPDITLVEAVTQALAYELAHDENVVVFGEDVGKNGGVFRATVGL
                     QDRFGENRVFDTPLAESMIAGLAVGMSIQGLKPVAEFQFMGFIYPAMNQIISHAARMR
                     NRTRGRLHCPLVYRAPFGGGIRAPEHHSESTEALFAHIPGLQVVIPSSPKRAYGLLLA
                     AIRNPDPVIFLEPKRIYRLVKQPVPDDGQALPLGKCFTLQQGDDLTLISWGASMHETL
                     QAAKQLTDEGISCDVIDVATIKPLDIETILSSVEKTGRCVIVHEGAKTCGVGAEISAQ
                     IMEHCMADLLAPVQRVTGYDTVMPYFQLEKQYIPSIARIKNTVMSIME"
     gene            4226..5338
                     /locus_tag="ACF2SP_002527"
     CDS             4226..5338
                     /locus_tag="ACF2SP_002527"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HFD2360301.1"
                     /translation="MNIFNLPDLGEGLPDAEIHEWFVKEGDTVKADQPLVSMETAKAV
                     VDVPCPQSGTIAKLYGKPGDVIKTGEPLVAFVSTTEKPADKGTVVGNLEESTDVSEDN
                     FIIGSQRSSHRVKTTPAVRMLAKKLGVDLSSLKGSGDNGVITREDVQNQANINSQPPA
                     GFEPLRGVRRAMLNSMVQSHAEIVPVSIFDEADIHIWKPNTDITVRLIRAIIHASKKE
                     PALNAWFDTKHAARRCFKEVHLGIAMDNEEGLFVPVIHDAEKYSDSELRKIIDEFKES
                     VRNRAVSADKLKGATITLSNFGKFSGRFASPIIVPPMVAILAVGRLYKAPVINDEDKI
                     EAHKMLPLSLSFDHRAITGGEATRFLGAVIESLQQA"
     gene            5525..>6960
                     /locus_tag="ACF2SP_002528"
     CDS             5525..>6960
                     /locus_tag="ACF2SP_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFD2360302.1"
                     /translation="MTMRIFVMILLFFFSISPTLGDSTLPAIFDVKNANKQFDQINLQ
                     LSVQNLNLNNLNKAVETLGELSAQSDECIADIQKKINGVDVLIKQATSTGNDKGADLV
                     YLNNQKHQLAELQSQCRLFSIRAQEAIEAYKSAILQLRQEKTLARTLPLWQLASKIIN
                     SSDNTKWINHTKEKFLLQDLPVYLWFCIVAGSFFISILILSKLRQSNVIRRYVKLKKI
                     RWSHITLLALFMMTGSFYGYISLFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVK
                     KIKAFFDWYAMDSEFFEILSIVIVAFYFLALSEQRLFDIWEVNKIIVELTRSIFLFLL
                     LGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFALLSGCAILNINGFYTLAMQLTLSG
                     IFTFFILYITILIGQSINKFYASLSYSQGKLNVIRIFGYKKDQILTEFYILKVTLKLV
                     IVLLSVYLIGISWGFATDFIDTVYKKLL"
BASE COUNT         2147 a         1206 c         1340 g         2267 t
ORIGIN      
        1 ataaataaga gaagttgtag taacttcata aactatatcc tgtaatttag gaagtttgtt
       61 taaacataat catggtacaa aaaatttgat ttgtacaatt tgtagcatat gatttcttta
      121 atttggttta ataatcaggt aaaggcattt aaagaaaagc cttttattgt cctggtatca
      181 tacttaatat taagaagcta attgcctcat aattcttgct gtagcacccc atattaattc
      241 ttcattagca ataaattggc aggtgacaaa gtgttttcct tctttctcaa ttctaatttc
      301 tttataattt ttctgatttt ttactaaagg cataggcaca agaattaatt tactaacctc
      361 ttgtggattc atagtgtaag gaaatataga atcaatactg gcgtaccaag ggtgaatgac
      421 taaacccagt aaagtctttt caatgtttaa ttttctgatc agagttattc tgtctgatgt
      481 aacacctaac tcttcattaa gttctctcaa tgcagtgcta tatagatctt gatcatttat
      541 ttcttgaaat ccaccaggaa agcacacttc tcctgggtgt ttattaagca tctgacttcg
      601 cttggtgaga attaacgagt ctttcgaaag atcatataaa acgataactg atgaatgtgt
      661 tttgattgac tgttgggtat tatttgattc cattgataat tgcagtaatt tttgtgaaac
      721 attcacgata ttcatcttcc caatcggagt caataactat tccccctcct gctgccaaat
      781 acaaaacatt atccttggct gtcaccgtac gtatcgcgat attggcatcg aaacggccat
      841 gctgtgaaaa ataggctata caaccacaat agacccctct tttatagatt tcctgttcac
      901 tgataatttt catcgtctct aatttaggcg ccccggtaat tgaaccacct ggaaaacagg
      961 agaaaaatgc ttcaattgga tgaatatcct ctttacatag agctcttatg tcggaaacca
     1021 aatgatgtac tgcattgaaa ctctggatgt ggcaaagatt cattaccttt acactgccag
     1081 ccttggcaag tttccccaga tcgtttctta gcaaatcgac aatcatgaca ttttcagctt
     1141 tgtttttagg gcatttcaat aactgttctt ttaatttttt atctacaaca ggatctttcg
     1201 aacgatggat agtgcctttt atgggtgaag ttaacatctc accgttgtca taaagtaaaa
     1261 atcgctcagg agaaaagctt aatatgtcag cttcctcagt tctaataaaa gcagcatagg
     1321 ggacaaagtt gtttctgtta accctttgat acatatacca gggatctccg gtgaatttag
     1381 actgaaacgg ttgagtgaaa ttaacctgat aagagcgccc tgctctcagg gcttgttgaa
     1441 tggcattgaa tgagctccta tagttctctt ttgatattaa aggcaaaaat tcagcttgaa
     1501 ctgaaaattg taccgatttt gcgggttgat tattcaatat attctttatt tcatctacaa
     1561 cgcttttggt tgaagaatga ctgtttcttg caaataaagt gacctttttt aaatggtgat
     1621 caactatgat agcccaatca taaaatccga agtttaaaac aggtatatgc gccagatctt
     1681 tttggggata ttgttcatga ttatgtaata gtgtaccaaa atcgtatgaa acgtagccta
     1741 tggccccgcc ctgaaaagga agatcaagtt ctgactgttc tatttttagt ttatttttta
     1801 attctgttaa tactaaattt ttatcatgta tattgttctt aataaccatc ctctcatatg
     1861 gataagcact taaaatatca taacgcccca tcgatttgtc cgtactctga agcaggacaa
     1921 atccaggtaa acgactgaat ttttcatagg attcagatga aaaatcatgg tagtcaagtg
     1981 taataagagt actatcaatc aaattatcac ctcaagataa ggatgtgcag tgaaaaaaat
     2041 gcagtgtttt acatttggtc attttaacct aacttatcaa taaaaaacgt atatttttat
     2101 cttgggttcc tattaattta ctggttttta aattaatttg tgagtatcct atgccaacgc
     2161 gttgttgaca aggagccatg taatgacaac tgttgctcaa tttgaaataa catacacaca
     2221 atacctgaat gaacaaggta aattggtcgc tgaattacct ccattcgcag ataatcaatc
     2281 catacttaaa gaattgtata aaatcatggt actcacccgt acttttgata aaaaagcaat
     2341 tgcattacaa agaacaggga aaatgggcac ctatgcgcca attaatggcc aggaagctat
     2401 ttcgactgca attggacatg ctcttaaacc tgaagatgta cttgtccctt attacagaga
     2461 ttatgctgct caatttcaga gaggcgttaa aatgtcagag atactcgcct tttggggcgg
     2521 ggatgagcga ggaagtcatt attctaataa tgccgaagat ttgcctattt gtgtcccaat
     2581 tgcatctcaa tgtttacatg ccgcaggagt cgcatttgcc ttcaaataca gaaaccaacc
     2641 aagagtagct gtggtatgta ttggagaagg aggcacttca gaaggtgatt tttacgaagc
     2701 catgaatgtg gctggggcat ggaaattacc agtggtcttc gtagtaaata ataacaaatg
     2761 ggcgatttct gtgcctatag agaaacaaac tggctcacaa acaatagccc aaaaagcgat
     2821 tgctgctggt ttttctggcg ttcaaataga cggtaatgat atttttgctg caagacaggt
     2881 tataggggat gctattgaaa aggctcgtca aggtggtgga cctactctaa tcgaagcctt
     2941 aacctatcgc ttgtgcgacc atacaacggc tgatgatgca acacgctatc agccttctca
     3001 tgaagtagag gaggcaaaag ccaaagagcc tattgttcgt tttaaacact atttaatgca
     3061 acaaaatatt tggacatctc aggatgaaga aaagctggtt atagaatgca gtgaaacggt
     3121 ggaaaaagca gtagaggaat atttgaacac caaacctcag cctgttagca gcatttttga
     3181 ttatcattat gcagaattac cagaatattt aattgaacaa cgcgcaatag ccatggagga
     3241 gtattctcat gcctgatatt actttagttg aagctgttac tcaggctttg gcgtatgagt
     3301 tagctcatga tgaaaatgta gtagtctttg gcgaggatgt tggtaaaaat ggtggagtat
     3361 ttcgagctac tgtaggttta caagacagat tcggagaaaa tagagttttt gatactccat
     3421 tagcagaatc gatgatagca ggccttgctg taggaatgtc tattcaaggc ttaaaacctg
     3481 ttgctgaatt tcaattcatg gggtttattt atccagccat gaatcagatc atatctcatg
     3541 cagcgcgtat gcgtaacaga accagaggcc gcttacattg ccccttggtt tatcgtgctc
     3601 cgtttggagg tggaatcaga gcaccggagc atcattctga aagtacagaa gcattatttg
     3661 ctcatattcc gggtttgcaa gtggttattc cctcttctcc aaaacgcgcc tatggcctgt
     3721 tgttggcagc tatacgtaac cccgatcccg ttatctttct tgaaccaaaa cgaatttatc
     3781 gcttggtcaa acaacctgtt ccagatgatg ggcaagcttt gccgttaggt aagtgtttta
     3841 ccctgcaaca gggagatgat ctgacattaa ttagctgggg ggccagtatg catgaaacct
     3901 tgcaggcagc caaacaatta actgatgaag gtatttcctg cgatgttatt gatgtagcaa
     3961 caattaagcc tctggatata gaaactattt taagttctgt agaaaaaaca gggcgttgcg
     4021 tcatagtgca tgaaggagca aaaacctgtg gagtgggtgc tgaaatatcg gctcaaatta
     4081 tggaacattg tatggcagat ttactagctc ctgtgcaaag agttacggga tatgatacgg
     4141 ttatgcctta tttccaactt gaaaaacagt acatacctag cattgcacgt attaaaaaca
     4201 ctgttatgag tataatggag taataatgaa tatttttaat ctacccgatt taggtgaagg
     4261 tttacctgac gctgaaattc atgaatggtt tgttaaggaa ggcgacacag ttaaagcaga
     4321 tcaaccttta gtttctatgg aaacggcaaa agcagttgtt gacgtaccct gccctcaatc
     4381 aggcactatc gcaaaacttt atggcaaacc aggtgacgtt atcaaaactg gagagcctct
     4441 tgtagcgttt gtttcaacta ccgaaaaacc tgcagataaa ggcacagttg ttggtaatct
     4501 ggaggaaagt actgatgtgt cagaagataa ttttattatt ggaagtcagc gttcttctca
     4561 ccgcgttaag acaacacctg ctgtaagaat gttagcaaaa aaattaggag ttgatttaag
     4621 ctctttaaaa ggtagtggtg acaatggtgt tattactcga gaagatgttc aaaatcaggc
     4681 caatatcaat tcacaaccac ctgccgggtt tgagcccttg cgaggtgtta ggcgagccat
     4741 gcttaatagc atggtacaat ctcatgcaga aatagttcca gtcagtattt ttgatgaagc
     4801 agatatccat atttggaaac ccaacacaga cattactgtt cgtttaatcc gggccattat
     4861 ccatgcgtca aaaaaggaac cggctcttaa tgcctggttt gataccaaac atgccgcacg
     4921 tagatgtttt aaagaagtgc atcttggtat tgccatggac aatgaggaag gtttatttgt
     4981 tcctgttatc catgatgcag aaaaatactc cgacagtgaa ttaagaaaaa taattgatga
     5041 attcaaagag tcagttcgta atcgggcggt tagtgcagat aagctaaaag gggcgacaat
     5101 tacgttgtct aattttggaa aattttcagg acgttttgcc agtccaataa ttgttcctcc
     5161 aatggtggcg attttagcgg ttggaagact ttataaagcc ccagtaatta atgatgagga
     5221 taaaattgaa gcccacaaaa tgcttccttt atccttgagc tttgatcata gagctattac
     5281 cggaggagaa gccactcgat ttttaggtgc tgttattgaa tctttgcaac aagcatgatt
     5341 ttaatttatg ttgggcttcg taaatagcga ggcctaataa caaatactat tactacaact
     5401 atctgtcttt tctatataaa tttaaatgcg aaaaatttaa tcctagtgat attttaaatt
     5461 tccttcttga gattcaatcc ataagcaagt agagttaata gtttgttaat tggcaaggaa
     5521 gagaatgacc atgagaattt ttgtcatgat tttgcttttc ttttttagta tttccccgac
     5581 cttgggggat agcactttgc ctgcaatttt tgatgtcaaa aatgcaaata aacaatttga
     5641 tcaaatcaat ctccagttgt cagtacaaaa tcttaattta aataacctga ataaggcagt
     5701 agaaactctc ggtgaactgt ctgcgcaatc cgatgaatgt atagctgata tacagaaaaa
     5761 aataaatgga gtggatgtat taatcaaaca agctacctct acagggaatg ataaaggagc
     5821 tgatttagtc tatctgaata atcaaaagca tcagttagct gagttacaat ctcaatgtcg
     5881 attattctca attcgagctc aggaagcaat agaagcttat aaaagtgcta ttttgcaatt
     5941 aaggcaagaa aagactttgg cgcgtacctt accattatgg caattagcaa gtaaaattat
     6001 taattcatct gataatacca aatggataaa tcacactaaa gagaaatttt tacttcagga
     6061 tttgcctgta tatctatggt tttgtattgt tgcaggaagc ttttttatct ctatcctgat
     6121 cttgtcaaaa ttacggcaat ccaatgttat acgccgttat gtgaaattaa aaaaaattcg
     6181 ctggtctcat attacacttt tggctttgtt tatgatgacg ggttcatttt atggctacat
     6241 ttccctattt actggtgaag agcagctaat caattctcaa ttccttttaa taaaggagct
     6301 attttggttt ctttgcggca cattattcat tgtttgttta ttcaaagtca aaaaaatcaa
     6361 agcttttttt gattggtatg ctatggatag tgaatttttt gaaatactaa gcattgtgat
     6421 tgttgcattt tatttcctag cattatcaga acagagatta tttgatattt gggaagtcaa
     6481 taagattatt gtggagttaa ctcgctccat ctttcttttt ttattgcttg gtacaaccgc
     6541 ttattttact cactatttct gccgttctca tctgcatatt tcttttgtaa aaagacatta
     6601 taattttatt cggtacttta tttttgcact gctttcaggg tgcgcaattc ttaatattaa
     6661 tggcttttat actttagcca tgcagcttac tctatctggt attttcactt tttttatctt
     6721 gtatattact attttaatag gacagagtat taataaattt tatgcttcat taagttactc
     6781 acaaggcaaa ttgaatgtta tacgaatatt cggatataaa aaggatcaga ttcttactga
     6841 gttttatatt cttaaagtta ccttaaaact tgttattgta ctcctaagtg tctatctgat
     6901 agggatatcc tggggttttg ctactgattt tattgatact gtttataaga aattattgta
//
LOCUS       DAXLDT010000152         6855 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXLDT010000152 DAXLDT010000000
VERSION     DAXLDT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.152"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(12..1094)
                     /gene="ravQ"
                     /locus_tag="ACF2SP_002529"
     CDS             complement(12..1094)
                     /gene="ravQ"
                     /locus_tag="ACF2SP_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavQ"
                     /protein_id="HFD2360303.1"
                     /translation="MMRMFSVRRNPSAVMRKDLSLTSRPLLQAPLLKSTVNPQNTLKN
                     NELKERKFTETLPDSRFFSKLKLAEQDQFSHRKRKRFNYQKNFSAALSLPYSRKELDV
                     HRDEEDTLLSMVKKYVIEHANRRVDLFFYTLIAYYNTEIVPTNGYTDLQHGRGRNTNR
                     GRTITEACHSSLIPSLLDSTIYQNKGRLKHKSLLSGTHFMDSLNATVELPPFVNDFDD
                     ILEKTCRKKCLKILHEVSLGRINPMEGLTLFLKMMQDILIKFKEEAASENQSVLLQNS
                     LIRKQFISPNLVDLVINGTLLTTFSDSTQSASDDYIQLLLRMTSEEKALCEKDEKGKI
                     KLYLAKIMDIQNEILTSKNGEFVPGL"
     gene            1580..3067
                     /locus_tag="ACF2SP_002530"
     CDS             1580..3067
                     /locus_tag="ACF2SP_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2360304.1"
                     /translation="MQNKLLQFICIKKLCKQKKSYKSIIKFASLLLAKKQIICAFALV
                     SIIKSSWAAHHYDTIIIGAGVSGLTAAYHLHNAQQKVLIIEAKNRLGGRVYTSYDWGF
                     ATDLGASWIHAIENNPLLPLIGKQSIIINSYSNSDPVAMLTNYALYDSEGKPVSKQTQ
                     TLFSSLTKEFLRYCQTRSQMISFAQNLTTFAKQKKLTSEQLALLSYALENIYTYEFAD
                     NLTKLSRNVHSASEASIASGKNALVPEGYFQLFRSLTQHVPIHLNQIVSQINYGPDGV
                     NIITQHEKYHANQVIITVPLGVLKANAIKFHPALPKDKRAAISQLGMGSYEKLYLLFD
                     KVFWDKDKEWIGMLPQNEQEAFNIFNYYKYTKKPVLIVFTSGKLARDMEKEHLTEWVM
                     QHLRRIYGNNIPKPIKNKKTHWGSDPFTRGSYSYLPVNVDKSVIDTLAQPVANRLYFA
                     GEATSNTDPSTVHGAYLSGIRAAEEVLASIKHSGKNRDRDKAVNS"
     gene            2967..3917
                     /locus_tag="ACF2SP_002531"
     CDS             2967..3917
                     /locus_tag="ACF2SP_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360305.1"
                     /translation="MALIYQGYAQQKKYWPASNTLEKIETEIKPSIHKPILILEKVMY
                     TKKGNHLLINKEEEQEENQNTVFVPQTNSLALPEPQSYFCTAEQCSLNNPYAPAQGYT
                     RPDSLHFKTTPINSEIYEQVLKVSEETMKTETPFTSRVAGHLEKEDNHSDNWSGLIPD
                     ELYQMMLEAAKQGIFVSFLLTLNDEIINDYLKNHHYSQQQINWISQGIRALTLIAIGT
                     SPGIALGLSISTLLLTEHLGFDKTNVNYLTTSSVVAWGLLSSPFNLLNTSLVMASSIG
                     ASILTSKITQSSYQFVRNRFFSSKPEKEELEHEHILQKTI"
     gene            4128..5600
                     /locus_tag="ACF2SP_002532"
     CDS             4128..5600
                     /locus_tag="ACF2SP_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360306.1"
                     /translation="MAHDKTFFYQQAHSSLTLLNYVHHDGIGDFKHLLDFVFYFNNTP
                     VLKEIELICIVINSHKPGNPQSVYIEEQLENMSVAHKILIHDSINGHENLRQEIKKNR
                     PLRKQLQHTIGIIDIALNTSYKLVIQEFCKDKPPTITIAEIDLYATYDQPSLTSDPEK
                     RIDKSRYQADHVYIMGLGKGKVGLKLTGGLFNSPQKAREALQQLSDKRIKHLLLGQVD
                     EEPISQETIDAFLQNNHFIPGYLQDIDTIFGFISIFATNHKLNTERKNLIFYLNNYQN
                     LFKKNYYDEDTGRRYSQFTDLLFDKHFPWHNVFANSGIRSITIYNYAGQKPTEEEITL
                     NPEGQQITIITGIMLNDTDYQLLYDIPQHFVAASGDNTLENAINHGLLFLMQYKYQYE
                     TVREIAKIAERVKDILGFDEEEIREFHAYFGEAHHLISYSNEKSTKARSLLQSIDLIR
                     LSSNMRKVCAYLIENENFLHQLPEILQTDLLNISKPGMKQ"
     gene            complement(5677..6741)
                     /locus_tag="ACF2SP_002533"
     CDS             complement(5677..6741)
                     /locus_tag="ACF2SP_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HFD2360307.1"
                     /translation="MFEINDQRKKEYYTALLSKNTCYEGIFYVGVKTTGVFCRPTCPA
                     RKPKLENCEFFHTAQEALLASYRPCRRCQPLSHPNQVSDLVKHLVDAVEANPEKRWKD
                     SDFAALSVDVATARRQFKKRFGMTFVAYARARRMGMAMKQIREGDLVINAQIDAGYHS
                     GSGFRDAFAKIMGAAPSHFNEQHKVLKASWFDTPLGPMLAVADENALYLLEFVDRRGL
                     EREVERLRNKTKAVIIPGDSPVLKAIQKEMELYFSGKLRQFNTPTHLIGSPFQKKAWL
                     ALIDIPYGETRSYLQQAKVIGNPSAYRAVANANGANQLAIVVPCHRIINANGELGGYG
                     GGVNRKQWLLEHERRHIRGC"
BASE COUNT         2252 a         1355 c         1135 g         2113 t
ORIGIN      
        1 gttaacacat cttaaaggcc aggtacaaat tcaccatttt tcgatgtcaa aatctcattt
       61 tgaatgtcca ttatttttgc caaatacaat ttgattttgc ccttttcatc cttttcacaa
      121 agcgcctttt cttctgatgt cattctaagt aaaagttgga tgtaatcatc agaggcgctt
      181 tgtgtgctat ccgaaaatgt ggttaataac gtgccattga taaccaaatc caccaagttt
      241 gggctgatga attgtttcct tatcaatgaa ttctgcaata gtacagattg attttcgctt
      301 gctgcttctt ctttaaattt tattaaaata tcctgcatca tttttaggaa aagggttaat
      361 ccttccattg gattgattct tccaagtgat acttcatgca ggattttcaa gcattttttc
      421 ctgcaagtct tttccaggat atcatcgaaa tcattcacaa agggaggtaa ttctaccgta
      481 gcatttagac tgtccatgaa atgagttccg gaaagaagac ttttatgttt taatcttccc
      541 ttgttttgat agatagtact atcaagcaat gatggaataa gactgctatg gcaggcttca
      601 gtgatagttc tgcccctgtt ggtattacgc cccctgccat gctgaagatc ggtataccca
      661 tttgttggaa cgatttctgt attataataa gctatcaatg tataaaaaaa taagtcaaca
      721 cgacgattag catgttcaat gacatacttt ttcaccattg acagcaatgt gtcttcttca
      781 tcccgatgta catctaactc ttttcttgag tatggcagtg agagtgctgc actaaaattc
      841 ttttgataat taaatcgttt acgttttctg tgactaaatt gatcctgttc tgccaatttt
      901 aattttgaaa aaaaccttga atccggtaat gtttcagtaa atttgcgctc ttttaactca
      961 ttatttttca gagtgttttg agggttaaca gtactcttaa gcagcggcgc ctggagtaaa
     1021 ggacgggaag ttaaagaaag atctttgcgc attactgcgg aagggtttct acgaacacta
     1081 aacattctca tcatggtctt ctcccttaat agccattatg tcaccgtagt acctgctcaa
     1141 agcatcaagc gtctgttttt ccataaagac acttaaactt acttagtgag agttcacagt
     1201 ctggatgatc ttcaaagcca gctccctgtc taagccagaa catatactgt ctttttacag
     1261 tacataataa tctttaaaaa agcaaacatt aaaataactt aatattgaat gcttcataca
     1321 gacaagttaa ttgctatgct gattattatt tacaaatatt gatacttttt ttaatatgca
     1381 tcaattcaat aacaaggatt tactgtgtca taccagactt ttttctcgac ttgatcgtca
     1441 taattcaatc ttgcgcactc gcatgcagcc aagcaaatac aaaactctag ccagtcaagg
     1501 ttagatgcga agcttggttg ggcaaatcaa tttgtccggc taaacctata aattaaaata
     1561 gcgatgtagg aagaaaaaca tgcaaaacaa attattacag tttatttgca tcaaaaaatt
     1621 gtgcaaacag aaaaagtctt ataaatcaat aataaaattt gccagtttgc ttttagcaaa
     1681 gaaacaaatt atctgcgcgt tcgcattagt ctctattata aaatcgtctt gggccgctca
     1741 tcattatgac acaataatta ttggtgctgg agtatccggg ttaactgcag cgtatcattt
     1801 acataacgca cagcaaaaag tacttattat tgaagcaaaa aatcgtcttg gaggcagggt
     1861 atataccagt tatgattggg gtttcgccac tgatttggga gcatcctgga ttcatgccat
     1921 tgaaaataac ccattactgc cattgattgg taaacagtcg attattatca atagttacag
     1981 taactctgat ccagtagcca tgctgaccaa ttatgcctta tatgacagcg aaggaaagcc
     2041 agtatcaaaa caaacgcaga ctttattttc aagtttaacc aaggaattct taagatattg
     2101 ccaaactcgc agtcaaatga tctctttcgc gcaaaatctc actacctttg caaaacaaaa
     2161 aaaacttact tctgaacaac ttgccttgct gagttatgcc ttggaaaata tctatactta
     2221 cgaatttgct gataatttga ctaaattatc acgcaatgtc cattccgcat ccgaggcatc
     2281 catagcttct ggaaaaaatg cccttgtacc tgaaggctat ttccaacttt tccgctcact
     2341 tactcagcat gttcctattc atttaaacca aatagttagc caaattaatt acggacctga
     2401 tggtgtcaac ataataactc aacatgaaaa atatcatgcc aaccaagtca ttattaccgt
     2461 cccattaggc gtgctcaaag ccaatgcaat caaatttcat cctgctttac ccaaagataa
     2521 gcgcgcagca atctctcagt tagggatggg aagttatgaa aaattgtact tattatttga
     2581 taaggttttt tgggataaag ataaggagtg gattggcatg ctcccccaaa atgagcaaga
     2641 agcattcaat atctttaatt attacaaata cacaaaaaag cctgtactca tcgtatttac
     2701 cagtggcaaa ctagcgcgcg atatggaaaa agaacattta accgaatggg tgatgcaaca
     2761 tttaagacga atttatggaa acaacatccc aaaaccaatt aaaaataaaa aaactcattg
     2821 gggcagtgat ccattcacac gtggcagtta ttcttacttg ccggtaaacg ttgataagag
     2881 tgttattgac actctcgcgc aacctgtagc taaccgtctc tattttgctg gagaagctac
     2941 ctcaaacaca gatcccagta ctgtgcatgg cgcttatcta tcagggatac gcgcagcaga
     3001 agaagtactg gccagcatca aacactctgg aaaaaataga gacagagata aagccgtcaa
     3061 ttcataaacc catcttgatt ttggagaaag taatgtacac taaaaaaggt aatcatttgc
     3121 tgattaataa agaggaagaa caagaagaaa accaaaatac cgtatttgtc cctcaaacaa
     3181 atagcttggc tcttcctgaa ccccaaagct acttttgtac agcagaacaa tgctctttaa
     3241 ataaccccta tgccccagct caaggctata caaggcctga ttcattgcat ttcaaaacaa
     3301 ccccaataaa ttcagagatt tacgaacaag tacttaaagt ttcagaagaa actatgaaaa
     3361 ccgaaacacc gtttacaagt agagttgctg gacacctgga aaaagaagat aaccattctg
     3421 ataattggag tggacttatt ccggatgagt tatatcaaat gatgctggaa gccgcaaaac
     3481 aaggcatttt tgtctcgttc ctgctcactc tcaatgacga aataataaat gactatctaa
     3541 aaaatcatca ttattcacag caacagataa actggatttc tcaaggcatt cgagctttaa
     3601 ctttaattgc tattggtact tcaccaggaa tcgctctggg attatcgata agcacacttt
     3661 tgctgactga acatttgggc tttgataaaa cgaatgttaa ttatctcaca acaagttcag
     3721 tcgtagcttg ggggctatta agctcccctt ttaatttgct taatacctcc cttgtgatgg
     3781 caagttctat tggagccagt atattaacca gcaaaataac acaatcatcc tatcaatttg
     3841 tgcgtaacag gtttttttct tcaaaacctg agaaagaaga actggaacat gagcacatat
     3901 tgcaaaaaac aatctagcga taatttactg caaaaacttt atgaattact atgagattca
     3961 tcccaaaagg cttcacttct tcctgtgttt gggacaaaat aatcagagaa cacttcagat
     4021 tttataacca aaattctcaa gcatagagca aaaagatttt aaattagttg atatttactg
     4081 actaatattg actatattta tagcatgatt actttttgta cagaataatg gcgcacgata
     4141 aaactttctt ttatcaacaa gcacactcta gccttactct gcttaattat gtgcatcacg
     4201 atggaattgg tgatttcaag catttactcg attttgtgtt ttattttaac aacactccgg
     4261 ttttaaaaga gatcgaattg atttgtattg tcataaacag tcacaagccg ggcaatcctc
     4321 aatctgtgta tatagaagaa caattggaaa acatgagtgt tgcacacaag attttgatcc
     4381 acgactccat caatggtcat gagaatttac gacaagaaat taaaaaaaat agaccattac
     4441 gaaaacaatt acagcatact attggaatca ttgacattgc attgaatacc tcttataaac
     4501 tggttatcca ggagttttgt aaagacaaac caccaacaat cactatagcc gaaattgatt
     4561 tatacgctac ttatgaccaa ccatctctaa cttcagatcc tgaaaaacgt atcgataaat
     4621 caagatatca agctgatcac gtctatataa tggggctggg gaaaggaaag gtaggtctaa
     4681 aattaactgg gggattattt aactctcctc aaaaagcccg agaagctttg cagcaactca
     4741 gtgacaagag aattaaacat ttgttgttag gtcaagttga tgaagagccc atttcacaag
     4801 aaacaataga cgctttttta caaaataatc actttattcc tggttacctt caagatatcg
     4861 atacaatttt tggattcatc agtatttttg ccaccaatca taaactaaat acggaaagaa
     4921 aaaatctgat tttttattta aacaattatc aaaatctttt caagaaaaat tattatgatg
     4981 aagacactgg gcgacgctat tcacaattca ccgatttatt gtttgacaaa catttccctt
     5041 ggcataacgt ttttgcaaac tcaggtatcc gatcaattac catctataac tatgctggtc
     5101 aaaaacctac tgaagaagaa ataaccctga atccggaagg tcagcaaata accatcatta
     5161 ctggtattat gcttaatgat actgactatc aattactcta tgacataccg caacatttcg
     5221 ttgcagcttc aggtgataat acgctggaaa atgctattaa tcatggtctg ctatttttaa
     5281 tgcaatataa gtatcaatat gaaaccgtta gagaaattgc taaaattgca gaacgagtta
     5341 aagatatctt aggttttgat gaagaagaaa ttcgagaatt tcatgcctat tttggtgagg
     5401 ctcatcacct tatcagttat agtaatgaaa agtctacaaa agccagatct ttgttacaat
     5461 caatagattt aatacgtctg tcttccaata tgagaaaagt ttgcgcctat ttaatagaga
     5521 acgagaattt tttacatcaa ttacctgaaa ttttacaaac cgatttattg aatataagca
     5581 aaccaggaat gaaacaataa attgtatcaa tcactacaac ttctggtagg ttaacatgtt
     5641 tattctatac tcctttttga gcgtagcaaa attagtctag caacctcgaa tatgccttct
     5701 ttcatgctca agtaaccact gtttacgatt tacaccaccc ccataacctc ccaactcgcc
     5761 attagcgttg ataatccgat gacaaggaac cactatagct aattgattag caccattagc
     5821 attagccacc gccctgtaag ccgacggatt acctattact tttgcctgtt gcagataact
     5881 tcttgtttca ccatagggaa tatcgattaa cgcaagccat gccttttttt gaaaagggct
     5941 gccaattaaa tgggtaggtg tattaaactg tcttagtttt cctgaaaaat aaagctccat
     6001 ctctttttgt atagccttta acactgggga atctcccggg ataatgactg ctttggtttt
     6061 atttctcagc ctttcaactt ctctttccaa ccctcttcta tcgacgaatt caagtaaata
     6121 taaagcgttt tcatctgcaa cagcaagcat aggacctaaa ggtgtatcga accaggaagc
     6181 cttaagaacc ttgtgctgtt cattaaaatg agatggagcg gctcccataa tcttcgcaaa
     6241 agcatctcta aacccgctgc ctgagtgata tccagcatca atttgcgcat taatcactaa
     6301 atcaccttct ctgatttgtt tcattgccat acccattctt ctggcacgag catatgctac
     6361 aaatgtcatt ccaaatcttt tcttaaactg gcgccttgct gttgcaacat caactgataa
     6421 ggctgcaaaa tcactgtctt tccatctttt ttcaggatta gcctcaactg catcaaccaa
     6481 atgtttgacc aaatcagaga cttgatttgg atgtgacaga ggttgacaac gtctacaggg
     6541 tctgtaagag gctaatagag cctcttgcgc ggtatgaaaa aattcacaat tttccaattt
     6601 tggttttctt gcaggacaag ttggtcggca aaaaacacct gttgttttca ctccaacata
     6661 aaaaattcct tcatagcagg tatttttact caacagagca gtataatatt ccttttttct
     6721 ttgatcgtta atttcaaaca tcaccattcc tgacaagctt ttttactaat tattataaat
     6781 cttctttaaa atggtgaagc cgaaaatcag acagtgaatt tttaaaatta gaaaaggcgc
     6841 cagttatcca tgaga
//
LOCUS       DAXLDT010000153         6814 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXLDT010000153 DAXLDT010000000
VERSION     DAXLDT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.153"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            24..638
                     /gene="mavI"
                     /locus_tag="ACF2SP_002534"
     CDS             24..638
                     /gene="mavI"
                     /locus_tag="ACF2SP_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavI"
                     /protein_id="HFD2360308.1"
                     /translation="MSSVKLCFALFRLYCYWRKLSMGLSAQQKTAIKSYFESNYNLFF
                     GIPTERKEGDLTPNEHVMVLQYLRFYLLKLPIECLEEELVNKQINGDDTVGILLEDFL
                     GNALSPMNPDAYFHSQKNFIQMGNAEDPAKPEDIIQYLIEVIENMSQQMDHGYLKKES
                     DPRIEKSNSSYASKALKWGLFGGAALAITYAVSKLSDKSHTNSL"
     gene            865..1422
                     /locus_tag="ACF2SP_002535"
     CDS             865..1422
                     /locus_tag="ACF2SP_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2443 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360309.1"
                     /translation="MIRKSEMIRHFFIVLFFFVGFMNQVSALPWFENENDLRNNNKLR
                     YRAHDEIKKNQIDFSGRWSGQCDNNPAADLTIKHTREHISISYGFMEEKYIPGEVRAE
                     SSSRLSLSESGSTTVKWNQDNTALIFINYHLFINESGSLNVFFSKVSMALEQEQLVVK
                     GQHFQTGNTLNDIKKETISCIYDRK"
     gene            complement(1546..1956)
                     /locus_tag="ACF2SP_002536"
     CDS             complement(1546..1956)
                     /locus_tag="ACF2SP_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2360310.1"
                     /translation="MKPLIPYLLFNGTCREAMTFYQNCFGGELELMTYGDSPEGACDK
                     KADKNDIMHACLRQGGFTLMASDWPGGEAKQGNNVHLNIDCSTKSEIEALFKKLSQEG
                     TVVQALADTFWGAHFGMLIDRYNIHWMLNYSLKQ"
     gene            complement(2056..2529)
                     /locus_tag="ACF2SP_002537"
     CDS             complement(2056..2529)
                     /locus_tag="ACF2SP_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2360311.1"
                     /translation="MQKITPCLWFDDKAEEAVTYYISIFKNGSIEKISYYSKEGFEFH
                     KKPAGSVMTILFNLNGQQYMALNGGPEFQFNESVSLMVNCDTQDELDYFWKELSADGE
                     EGPCGWLKDKFGLSWQIVPDILAELLSDHQKSEKVMRVLLQMKKLDIAKLKAATE"
     gene            complement(2567..3526)
                     /locus_tag="ACF2SP_002538"
     CDS             complement(2567..3526)
                     /locus_tag="ACF2SP_002538"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFD2360312.1"
                     /translation="MGILIDGIWHDENNINTDKEGRFVRAESGFRQHITASGESGFKA
                     EPDRYHLYLSYACPWACRTLIMRKLKGLEKIISVSFSDPVMGKNGWSYGESGNETADE
                     INHIHYLKELYLLARKDYTGRVTVPVLWDKKKKTIVNNESSEIMRMLNSEFNAFSSTD
                     HDYYPKQWRQKIDEINQLVYENINNGVYKCGFAKSQKAYEEAFDALFNTLNQIENILA
                     TQRYLIGEQLTEADWRLFTTLIRFDAVYHGHFKCNLQRIIDYPHLSNYLRELYQFPGI
                     KETVNFNHIKQHYYRSHLSINPTGIVPKGPKLNLDEPHNRHEF"
     gene            3675..4226
                     /locus_tag="ACF2SP_002539"
     CDS             3675..4226
                     /locus_tag="ACF2SP_002539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFD2360313.1"
                     /translation="MFNVAVIVGSLRKESFNRKLAFALDKLQHPTFQLSHIKIDDIPL
                     YNQDFDNNLPDSVIRLKKEITEADAVLFVTPEYNRSIPGVLKNIIDWGTRPYGKNVWA
                     GKLMAAIGTSPGTIGTAVAQSHLRSIMVAVDGVYFGQPEIYLVYKDGLIDNNFSITME
                     STRNFLQGFLDRFAREIEKHKSI"
     gene            4493..5683
                     /locus_tag="ACF2SP_002540"
     CDS             4493..5683
                     /locus_tag="ACF2SP_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFD2360314.1"
                     /translation="MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFSTDS
                     TTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALI
                     FARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
                     TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVHQVMRQIISNSDFRQYT
                     WVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVANMMAARLTYSHS
                     LESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDF
                     EQQAGVATALLGACQFGLAGLIGVLTAQWMPGPFSVAIPVLCFTAVGLARITKFSFRI
                     LNGS"
     gene            5914..6342
                     /locus_tag="ACF2SP_002541"
     CDS             5914..6342
                     /locus_tag="ACF2SP_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009374 - biotin binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="avidin/streptavidin family protein"
                     /protein_id="HFD2360315.1"
                     /translation="MKIKWLCSSIFVASTLISLPNYAQQDILTFKNTRGSVLELRTLA
                     DNKVEGYFTTAVASKSCPQAIGQKRPITGYAVGNTVTFSVVYPVCGSVLNFSGNFTKK
                     GNIIDTISILTHQAQDITHEGPGARFIGHDRFKKVAASNT"
     gene            complement(6449..>6814)
                     /locus_tag="ACF2SP_002542"
     CDS             complement(6449..>6814)
                     /locus_tag="ACF2SP_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360316.1"
                     /translation="VKTVCIFLSIILYVIALCLPAAIIPDFERGIHQTHYGFEILITG
                     WMGFLAWQPAWLANLLYLFSLLTYGSKSSYRFGTGAFLLALVSPFILVEKLLIGFYVW
                     LVSFAVLLYGNNLHEMTTE"
BASE COUNT         1985 a         1247 c         1315 g         2267 t
ORIGIN      
        1 atcatgaaag atttaagatc actctgtctt ctgttaagct ttgctttgca ttatttagac
       61 tttactgcta ttggaggaag ctcagtatgg ggttatcagc ccagcaaaaa acggccataa
      121 aatcttactt tgaatccaat tataatctgt tttttgggat tccaactgaa cgtaaagaag
      181 gcgatttaac acccaatgaa catgtgatgg tattgcaata cctgcgattt tatttattaa
      241 agttgcctat tgagtgtttg gaagaagagt tggtgaacaa acaaatcaat ggtgatgata
      301 cggtaggtat tttattagag gattttctgg gcaacgctct ttctccaatg aacccggatg
      361 cttattttca tagtcaaaaa aattttatcc agatgggtaa cgctgaagat cctgcaaagc
      421 cagaagatat tattcagtat ttaatagaag tcatcgagaa tatgtcgcaa caaatggatc
      481 atggttattt aaaaaaagaa agtgatccca gaatagaaaa gagcaacagt tcttatgcgt
      541 cgaaagcact aaagtggggc ctatttggag gagctgcttt agctataaca tatgctgtca
      601 gcaaattgtc tgataaaagc cataccaata gtctgtgaaa gcgacaatat tcagtttcgg
      661 ggtaacggaa tatcaatttc cacaggattt ctcgaagtac gtcttttacc aaagcaaaac
      721 catgttatgt tgtaagagtt gtgttaaaag caaggaaccc gaattgctat ttccctggat
      781 actccttaaa aagtagattg caatgatatt ataaattcgt tttctgcgag acagaaaaag
      841 tattattctg tacaaacctg cctactgata aggaagtcag aaatgataag acattttttt
      901 atagttttat tctttttcgt cggttttatg aatcaggttt cagctcttcc ctggtttgag
      961 aatgagaatg atttaagaaa taataacaaa ctgagatata gggctcatga tgaaatcaaa
     1021 aaaaatcaaa tagatttttc tggcaggtgg agcgggcaat gtgataataa tccggctgcg
     1081 gacctaacta tcaagcatac tcgagaacac atttctattt cctatggatt tatggaagaa
     1141 aaatatattc cgggtgaagt tcgagcagag tcatcctctc gcttatcttt atctgaaagc
     1201 ggcagcacca cagtgaaatg gaatcaagat aacacagctt taatttttat taattatcat
     1261 ttgtttatca atgaaagcgg tagtttgaat gtgtttttta gtaaggtttc catggcatta
     1321 gagcaagagc aactggtggt caaagggcag cattttcaaa caggaaatac cttgaatgat
     1381 attaaaaaag aaaccatctc atgtatttat gaccgtaaat aatgacatga agtaaatccc
     1441 tgtatgatga gctttttgct ggtcacatgg gatttacttt actggttagt gaaacgacat
     1501 caattgatta aaaattgtcg atcagacttt tttgtatttt gatttctatt gtttcaaaga
     1561 ataatttaac atccagtgta tattatacct atcaatcagc ataccaaagt gcgcacccca
     1621 aaatgtatcg gcgagtgctt gcaccaccgt tccttcctga ctgagttttt taaacagagc
     1681 ttcaatttct gattttgtgc tgcaatcaat gttgagatgc acattattgc cttgctttgc
     1741 ttcgccacct ggccaatcag atgccatcag agtgaaacca ccttgtctta aacaagcatg
     1801 catgatgtcg tttttatcgg cttttttatc acaagcccct tcaggggaat caccatacgt
     1861 cattagttcc aattctcctc caaaacaatt ctgataaaat gtcattgctt ctcgacaagt
     1921 accgttgaaa agaaggtaag gtattagcgg tttcatgtat tctccttgat aaattgattg
     1981 ttgcaagact tgtagcccca atttctttgc tgaaaatcat ttagtgcctc gatctaggga
     2041 tttttcgtta gcatcttatt ctgttgcagc tttgagcttt gcgatatcca gttttttcat
     2101 ttgcagaagc actctcatga ctttttctga cttttgatgg tcactcagca attcagccag
     2161 gatatcggga actatctgcc aagataatcc aaatttgtct ttgagccagc cacaaggacc
     2221 ttcttctcca tccgctgaga gctccttcca gaagtaatcc aattcgtctt gcgtatcgca
     2281 attgaccatc agtgagacgg attcgttaaa ttgaaattca gggccgccat taagtgccat
     2341 gtattgttgt ccattcaaat tgaagagaat tgtcatcaca ctgcctgctg gttttttatg
     2401 gaattcaaat ccttctttgc tatagtaaga aattttttca atactcccat ttttgaaaat
     2461 cgagatataa tatgtcactg cttcttcggc tttatcatcg aaccatagac aaggagtgat
     2521 tttttgcatg gttttttatc cttatttcta gctgttgaaa aatttattaa aattcatggc
     2581 ggttatgtgg ttcatccaaa tttaattttg gacccttggg aacaattccc gtaggattaa
     2641 tgcttaaatg acttcgataa taatgttgct tgatatgatt aaaatttacg gtttctttta
     2701 taccaggaaa ttgatagagc tctcgtaaat aattggataa atgcggatag tcgataatcc
     2761 gctgcaagtt gcatttgaag tgaccatgat acactgcatc gaaccgaatt aatgttgtaa
     2821 ataaacgcca gtcggcttcg gtaagttgct ctcctatcaa atatcgctgt gttgccaata
     2881 tgttttctat ttgatttaag gtattaaata acgcatcaaa tgcttcttca taagcttttt
     2941 gggatttagc gaagccgcat ttgtaaacac cgttgttaat attttcatag acaagctgat
     3001 taatctcatc aattttttgg cgccattgtt ttggataata gtcatgatct gtagaactaa
     3061 aggcattaaa ttcgctattc aacatgcgca tgatttctga tgattcatta ttgactatcg
     3121 ttttcttttt tttgtcccat aaaacaggaa ctgttactct accggtataa tcttttctgg
     3181 caagcaaata caactctttg agataatgga tatgattgat ttcatcggct gtttcattac
     3241 ctgattcgcc gtaactccag ccatttttac ccatgactgg atctgaaaaa gaaacagaaa
     3301 taattttttc caatcctttc aattttctca tgattaacgt tcggcatgcc cagggacaag
     3361 cataggacag gtataaatga tatcgatcgg gctctgcttt aaacccactc tctccactcg
     3421 cagtaatgtg ctgacggaaa cctgattcag cacgaacaaa acgtccttct ttgtccgtgt
     3481 ttatattgtt ttcatcgtgc caaataccat caattagtat gcccattatg aattccttat
     3541 atatcaataa tttattaata ttttttgaat actggcgcag catgaatacg tcttgtttaa
     3601 taactgcttt ttaatgcata atcattgata aatcgattaa ttcattttgt atcaataaat
     3661 ttaaggaaaa atttatgttc aacgtagctg ttattgtcgg aagccttcgt aaagagtctt
     3721 ttaatcgtaa attagcattt gctctggaca agcttcagca tcctacattt cagctatctc
     3781 atattaaaat agatgatatt cctctttata accaggattt tgataataat ttacctgact
     3841 cagtcattcg gttaaaaaaa gaaattactg aggcagatgc cgttttattc gtgacacctg
     3901 aatataaccg ttcgataccc ggtgtattga agaatattat agattggggg accaggccat
     3961 acggaaaaaa tgtatgggca ggcaaactca tggcagctat tggaacgagt ccgggaacta
     4021 ttggaaccgc tgttgctcaa tcgcatttgc gaagcataat ggttgcagtt gatggcgttt
     4081 attttgggca gcctgaaatt tatcttgttt ataaagatgg actgattgat aacaattttt
     4141 ctattaccat ggagtctacc cgaaactttc tgcaaggatt tctggatcgt tttgccaggg
     4201 aaattgaaaa acataaatca atataagtat gtcgactgat tacccaaaac aatgtttctg
     4261 gtaattggtg catttttcta tgtttccgat cattcatttt ggctaaagcc tcttgcccac
     4321 ttcgttgttc cttatgtttt aaggtagtcc agaattttag cgtttggact accaataatt
     4381 aattgaataa catctgttga atcagcaatt ttataacatt gagtgtaaat ctgttacaat
     4441 taagcgcagt ttatattttt catgagtaat attttaacag gaataccatt aattgaattt
     4501 tattggctgg acagaaagac aaagtctttt tcgattattt ccactcatta tcagcatttc
     4561 ttttgctatg gatgtttttg tgcccgcaat tccggaaatg agtcgttatt tcagtactga
     4621 cagtacaacg atgcaagcta ccctttattt gtttatgctc actgtggctt tgggtcagct
     4681 attggttggg cctttggccg atcgttttgg acgaagacag atggctttga gtacggcact
     4741 tctttttttt gcaggatcgt tgatctctgc gttagctccg tcagtaccgg cattgatttt
     4801 tgcacgtatc attcaggcag ctggggcctg tggaacctat ttactatgct ttattatcgt
     4861 tcgagataat ttttcaacgg gcacctgtgc ccgcttgttt agtatcctgt ctgggacgaa
     4921 ttcgatggtt gccagttctg ctccagtcat tgggggatta ttgttggata tgacgcaaga
     4981 ttggcatagt ggattctact ttttaacctt gcttgggtta ttaatgagtg gcatagcctt
     5041 ccgttacatc cccgattatc actatcccaa acccgagctg ccccagatga gggtgcacca
     5101 agttatgagg cagataataa gtaactctga ctttcgccaa tacacatggg ttgcatcagc
     5161 cagtttgctg ggattgtact tgttttgtgc tcaatcacca ggtattttga tcacacaact
     5221 gcacctgagt gcgactgaat atggtttctg gtttggtttg aatgcaatga ctgtgtttgt
     5281 tgccaatatg atggccgcac gacttaccta ttctcactca ttggaaagca tagtgcgttg
     5341 gggcttggtt ttaatgacac tgtcatgctt ttttatgctg gctcttaatc ttcgtcaatg
     5401 cagcatttta agttttatgc ttcccatgct ttgcttgact gtgggtattg gtattagtat
     5461 gggcacagca acagcgcttg ctttaaagga ttttgaacag caagctggtg tcgctaccgc
     5521 tttgttgggt gcttgccaat ttggtctcgc gggcctgatt ggtgttttga cagctcaatg
     5581 gatgcccggg ccatttagtg tagctatccc cgtactttgt ttcactgcag tcggattggc
     5641 tagaatcacc aaattcagtt ttaggatatt gaacggctct taattgattc aaaatgttgt
     5701 tctacaattc gggtaacttc agcgcatgta tttaaatgga ttttcttctt gcaatcaagg
     5761 tgaaaaatcg agcagaattg atagccaaat ttaatcgtaa ctacccgtga tttcacggag
     5821 atacaacaat tactttatgt aataaagtag gttagccaga aataacattt ttggcgtttt
     5881 gaatgaacat tatctaagtt tcaggagtta ttaatgaaaa taaaatggct gtgtagcagt
     5941 atttttgtag cctccacatt gattagtttg cctaattacg ctcaacaaga cattctaacc
     6001 tttaaaaata ctcgtggctc agttttagag ctgagaactc tagctgataa taaggttgaa
     6061 ggctatttta ctacggcggt tgcttctaaa tcttgtccgc aggcaattgg ccaaaaaaga
     6121 ccgattaccg ggtatgcggt aggtaacact gtaaccttca gtgttgttta tcctgtatgt
     6181 ggttctgtat taaattttag tggcaatttc actaaaaaag gaaacatcat tgataccatt
     6241 tctatcttga ctcatcaagc gcaggatatc actcatgaag gtcctggcgc tcgtttcatt
     6301 ggacatgatc gctttaaaaa agtagctgca tccaatacat agaatgattt ttccaagcaa
     6361 gcttaaaata agcctcatgt caagaaatga ggctttaatg attctgtaga tgttctatag
     6421 acaaggaatg aatttggtaa tcttaaatct attctgtagt catttcatgc aaattattcc
     6481 catacagtag gacagcaaaa ctaacaagcc acacgtaaaa cccgattaat aatttttcta
     6541 caagtataaa aggtgaaacc aatgccagaa gaaaagcacc ggtaccaaaa cgataactgc
     6601 ttttagagcc ataggtaagt aatgaaaata gatacaaaag gtttgccagc caagcaggtt
     6661 gccatgctag aaaacccatc caccccgtta ttaagatttc aaatccataa tgtgtttgat
     6721 gtatccctcg ttcaaaatca ggtattattg ccgcaggcaa gcatagggca atgacataaa
     6781 ggattattga taaaaagata catactgttt taac
//
LOCUS       DAXLDT010000154         6800 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXLDT010000154 DAXLDT010000000
VERSION     DAXLDT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.154"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1588
                     /gene="sidK"
                     /locus_tag="ACF2SP_002543"
     CDS             <1..1588
                     /gene="sidK"
                     /locus_tag="ACF2SP_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector v-ATPase inhibitor SidK"
                     /protein_id="HFD2360317.1"
                     /translation="REDYQDVLIWAEKNYRFEEILEASKSGKCPNDLDALSRRSLILQ
                     ELLRLVSSISPFKMKLDLIESQYEKMKQHVNLWKSDYHVKLNQLNQLTDYLKNAAPTP
                     KNNFLRAMTSVLQMQIAQYGITEDNEGINQLFKLGLHLLAMANEKIDEQYHLFKGYVK
                     DQAEESPFEGILPEEDQKILVKGMIDYAMPKLSSKVLQDKLSALSSSDVLTKTLLDSI
                     DRIVEENEKLNALSKVKLGKYGLDIREIEEIYSQALKISPQDALQYTAQQCDAQLLSM
                     AFPDSQNYIVESISDKKAKAIAELIHSKEFIYQIIKTEVFKQVDPNEKIRLQAATELY
                     QLLGRIMDKQIHLFAKMNLEQINEYIQTKTKAILDKIPERVESLTFMGFEIPTFKGIE
                     TLMTDISHSQDNETLAIAQEFYTNIKNAKKQLLGDKLIEDITPQDVEKFFNQCSQYGS
                     EAAEKLADNRPVLTKIADILTAIARWAISLIGFNTPPQFLAPTRTCVDQVSDEITKIK
                     LKLEDTLGSLQKVQEENLSL"
     gene            1872..2465
                     /locus_tag="ACF2SP_002544"
     CDS             1872..2465
                     /locus_tag="ACF2SP_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0967 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360318.1"
                     /translation="MLHFKYRYMSGVDIMTRSNSEQLEEEYLNARREYCMAMMAVHDK
                     ARTLLSETPIDGSMKQAIIKSSGVKIRPNETQDEFDERFEDEMGLTIDDYVDFALDVL
                     EKKLEPARQSIVAAANKELGSLTSGPSGSAWEDFQHTIHEGAEYKTIEVAKCLPESER
                     CIQAFNQAKLTFRDAATNLIHAEKLEQPEEPNHGPRF"
     gene            complement(2611..4488)
                     /locus_tag="ACF2SP_002545"
     CDS             complement(2611..4488)
                     /locus_tag="ACF2SP_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFD2360319.1"
                     /translation="MVFIMQKIIILTKKIIAKLPVILFDVAAIPVAWYAAYWLRYNMQ
                     PYPSSLTSTHSFIALALLSIVQISCYYYFKIYRGLWRFSSLNDVIRILKATITAMVLV
                     IPVFYLTSILQHLPRSVFPLYCIILATILCGGRLVIRLHWDKPGRGNRELETKRVLVV
                     GAGQAGEGLVRDLKRSSYYRPIGFIDDNKSKRGLEVHGVRVLGTTNQITELVNQYDVD
                     LIFIAIPSAKSATMRRIVTLCEQSHVPFSTLPSISALAAGRVEVNALRPVNIEDLLGR
                     DQVTLEWEKIARSIAGRRILITGGGGSIGSELCRQVMALEPASIAIVENSEFNLYRIE
                     QELLKNFPGIPVELSLISVTDEIAINHLFRRFQPEIVFHAAAYKHVPMLQHQVRVAVF
                     NNVIGTQVVAKASVTFGAEKFILISTDKAVNPTNIMGTTKRVAEIYCQNLNTRVKTQF
                     ITVRFGNVLGSAGSVVPLFQKQLQEGGPLTVTHPDMQRYFMTIPEACQLILQAMVNGE
                     GGEIFVLDMGEPVKISYLAEQMIRLAGKEPGRDIMIEYTGLRPGEKMYEELFHETEQL
                     TSTKHEKLFKARFRELDWNDLTQTMRMLQAACIEHQDEELLVLLKSLVPEFQLEPMVE
                     A"
     gene            complement(4543..6090)
                     /locus_tag="ACF2SP_002546"
     CDS             complement(4543..6090)
                     /locus_tag="ACF2SP_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HFD2360320.1"
                     /translation="MVLRYPDLHGIELMSAKWRWYDSLFILLFAYLLVLQIQAIWLFT
                     IDDMYISLRYAKNWAAGNGLLWNLNAEPVEGYSNFSFVALGAISIILHINPVLTLKIA
                     GFAGLLATCLFIFLITRFWFTKRESFIPCIWLLLYKGQVIWSVSGLETTVYQALISGS
                     VYFAFRGLGYHFYPAPRGEFKTSGFVLSGLYLSFAGMTRPETPALMLLFFLLIGWDWN
                     KLSGKKPWRGAVSFAVTIGLVFLPYFLWRWSYYGLLFPNSVYCKGFNGISYSLDLNYL
                     KLIWPFAILALPACFYAQDRRHYFLWSPGIIYLLMLVNADPVVAFDNRLFLPAFALML
                     PLTLNGISYLLLSYFKKQDNYFRAALYVSSFFLALLFIPKMSLDAYRYFSQNPVHGEQ
                     LRKSVVQWLNTHAKNQDRVVLADSGLIPFLSGLTFIDSYCLNNKEMAQFPAVKRYELF
                     CQQILEEKPQIIILTALIDKGKIIYTPGDSCLKPLLDVNKEYKLGGVFSFGSAESQYR
                     YELFANF"
     gene            complement(6051..>6800)
                     /locus_tag="ACF2SP_002547"
     CDS             complement(6051..>6800)
                     /locus_tag="ACF2SP_002547"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HFD2360321.1"
                     /translation="GYLFPALMASLSFCLIASAVYIYNDIQDRTEDSLHPFKCNRPIA
                     SEQVTLFDAIIVMLLLMVAGIALGWLISKKLVVILIIYLIINLAYNHVLKLIPVFDVA
                     CIALGFMLRVLAGTIGIDLAISAWLTVAATLLSLFIALNKRQLEMQLGLKNSTRKVLR
                     RYNPVFLQRLIVITGIACFITYLFYTVYARAESFFFILTLPFAAIALWRFAWLATQDV
                     ENDDPVNIFLSDRLSRINLWCFAILTFMALN"
BASE COUNT         2372 a         1378 c         1241 g         1809 t
ORIGIN      
        1 aagagaagat taccaagatg tattgatatg ggcagagaaa aattatcgtt ttgaggaaat
       61 attggaagcc agcaaatctg gaaaatgccc taatgatctg gatgcattaa gtcgacgatc
      121 gctaatactc caagaattgc tgcggcttgt ttcatcaatt agtcccttta aaatgaaatt
      181 agacctgatt gagtctcaat atgagaaaat gaagcagcat gtgaatttat ggaaaagtga
      241 ttaccatgta aaactaaatc aactgaatca attaacagat tacctcaaaa atgcagcacc
      301 aactcctaaa aataattttc taagagcaat gacctcagta ttgcaaatgc agatagccca
      361 atatgggatt accgaagata atgaaggcat caaccagttg tttaaactgg gattgcattt
      421 gctcgcaatg gctaacgaaa aaatagatga acaatatcac ctgtttaaag ggtatgtaaa
      481 agatcaggct gaagaatctc cttttgaagg cattttgcct gaggaagacc agaaaatttt
      541 agtcaaaggt atgatagatt atgctatgcc gaaactttca agcaaggttt tacaagataa
      601 attatcggcg ctatccagtt ccgatgtatt aacaaaaaca ttacttgata gcatcgacag
      661 aattgttgag gaaaacgaaa aattaaatgc tttaagcaaa gtcaaattag gtaaatatgg
      721 cctggatatc agagaaattg aagagattta ctctcaagct ctgaaaatat caccacaaga
      781 tgctttgcaa tataccgctc aacaatgtga tgcccaatta ttaagcatgg catttcctga
      841 ttcccaaaat tacattgttg aatctatatc ggacaaaaag gccaaggcca ttgcagagct
      901 aatccatagt aaagaattta tttatcaaat tattaagaca gaagttttta aacaagttga
      961 tcctaatgag aaaattcgac tacaagctgc gacagaattg tatcagcttc ttgggagaat
     1021 aatggataaa cagatccatc tttttgccaa gatgaatctc gaacaaatta atgaatatat
     1081 tcaaaccaaa accaaagcaa tcctggataa aattcctgaa agagttgagt cattaacttt
     1141 tatgggcttt gaaattccaa ctttcaaggg aattgagaca cttatgaccg acatctccca
     1201 ttctcaggat aatgaaactc tggcgatagc ccaagaattt tatacaaata taaaaaatgc
     1261 aaagaaacaa ttgttaggtg ataaactgat tgaagacatt actccacagg atgtagagaa
     1321 attttttaat caatgtagtc aatacggttc agaagcagct gaaaagttag ctgacaatcg
     1381 tccggtattg acaaaaatag ccgatatttt gacagccatt gcccgctggg cgattagttt
     1441 aataggtttc aacacgccac cgcagttttt ggctccaacc agaacctgtg tggatcaggt
     1501 atctgatgaa atcactaaaa ttaaactcaa acttgaagat actttgggaa gcttgcaaaa
     1561 agtacaggaa gagaacctaa gcctttaata acgattaagt ccacaataaa aaatacttga
     1621 tataagaatt tacctgttat cagagatgtt ctcccggatt acagctttct tgcaatccgg
     1681 gggctcttta taatgagata atgcatcacc atactaccta agctcatggg gcgagagagt
     1741 agatcgaagt gaaattaaaa tccccgctca attcaagatt aaatgttcac taaaaattta
     1801 gggatattga cttagtatgc accacaaccc gattggtatt tttactttca ttttgaataa
     1861 ttataaatca aatgctacat tttaaatata ggtatatgag tggggtagat atcatgacaa
     1921 ggtcaaatag cgaacaactt gaggaagaat accttaacgc aagaagggag tattgtatgg
     1981 cgatgatggc tgtacacgac aaagcaagga cattgctgag tgagactcca atagatggaa
     2041 gtatgaagca agctataata aaatcaagcg gggtaaagat caggcctaac gagacacaag
     2101 atgaattcga tgagcgcttc gaagacgaaa tgggtttaac gatagatgac tacgttgatt
     2161 ttgcgcttga tgtattagaa aaaaaattag agcctgcaag acagagtatt gtagctgctg
     2221 caaataaaga gcttggttct cttacctctg gaccatccgg ttcggcatgg gaagattttc
     2281 aacatacgat ccacgaggga gcagaatata aaactataga agtggctaag tgtctccctg
     2341 agtctgagcg ttgtatacaa gcttttaatc aagctaagct gacatttcgt gatgcagcca
     2401 caaatttaat acatgcagag aaactggaac aaccagaaga gccaaatcac ggccccaggt
     2461 tctaaaacaa aggtccagaa agatgtaaga agctaagaaa ttcttttgct gtttttcatt
     2521 ttaccatcaa attatgagaa aatagcgttt caaaatcaac ataagtaatt gccataacac
     2581 aatcttgatt taccagatta ggtatattgc ttaagcttca accataggtt ccaattgaaa
     2641 ctcaggcacc agacttttta acagaacaag caattcctca tcctgatgct caatacaagc
     2701 cgcctgcaac attcgcatgg tttgtgtcaa atcattccaa tccagttcac gaaaccttgc
     2761 tttgaataat ttttcatgtt ttgttgaagt taactgttct gtctcatgaa acaactcttc
     2821 atacattttt tcgccaggac gcaaccctgt atattcaatc atgatatctc ttcctggctc
     2881 tttgcctgcc aagcgaatca tttgctctgc aagatagctg attttaaccg gctcccccat
     2941 atccaggaca aaaatttcac cgccctcccc attcaccatc gcttgcaaaa tcaactggca
     3001 tgcttccggt atagtcataa aatatctttg catatcggga tgagtcacag tcagtgggcc
     3061 accttcctgc aactgttttt ggaacaaagg cactacacta ccagctgaac ccaagacatt
     3121 cccaaaacga accgtaataa actgggtttt aactcgtgta tttaaattct gacaataaat
     3181 ttcagcgact ctcttggtag ttcccattat attcgttgga ttcactgctt tatcagtgga
     3241 aattaaaatg aatttctcag ctccgaatgt cacactggct ttggcaacaa cctgagtacc
     3301 tatgacatta ttaaacacag caacccgtac ttgatgctga agcatgggaa catgtttgta
     3361 tgcagccgca tgaaatacaa tttcaggttg aaaccgacga aataagtgat taatcgcaat
     3421 ttcgtctgtc acactgatta aactcaattc aacggggata cctggaaaat tctttaacaa
     3481 ctcctgttca atgcgataca ggttaaattc actattctca acaatggcta tgctcgcagg
     3541 ctctaaagcc ataacctgac ggcacaattc cgaaccgatt gagccaccac cacctgtaat
     3601 caatatgcgt cttcctgcga ttgaacgagc aattttttcc cattcgagcg tcacttgatc
     3661 ccgacccaat aaatcctcaa tattcacagg cctcagcgca ttcacttcaa ctcggcccgc
     3721 agccagggct gaaatactgg gtaaagtact aaaaggcaca tggctttgtt cacaaagcgt
     3781 tacaattctt cgcatagttg ccgatttggc agaaggtatg gcaatgaaaa tcaaatccac
     3841 atcgtactga ttgactaatt cggtaatttg gttggttgtt cccagaaccc gcactccatg
     3901 aacttctaat cctctcttgc ttttattgtc atctataaag cctattggcc gataataact
     3961 gcttcttttc aaatcacgca ccaagccttc accagcctgc ccggcaccaa ccaccaaaac
     4021 tcttttcgtt tccaattccc tgtttcctct gccaggctta tcccaatgca ggcggatcac
     4081 caaccttcct ccgcaaagga tggtagccag gataatgcaa tacaaaggaa aaactgatct
     4141 gggcaggtgc tgaagaattg aagtcaagta aaaaacaggg attactaaaa ccattgctgt
     4201 gatagtagcc ttcagtatcc taatgacatc attcagagaa gagaaacgcc ataatcctct
     4261 gtaaatttta aaataatagt aacaactaat ctgaacaatt gataataaag ctaaagcaat
     4321 aaaagaatgg gtggatgtca ggctgctggg atatggctgc atgttatagc gcaaccagta
     4381 agcggcatac caggcaactg gtattgcagc cacatcaaac aaaattactg ggagttttgc
     4441 aatgattttt tttgtcaata tgattatttt ttgcattata aaaaccacag tctgtaatcc
     4501 tttataaagg aaagatggta cactatgtta cagtttctcg catcaaaaat tagcaaacaa
     4561 ttcataccta tattgtgact cagcactgcc aaaagaaaaa acaccgccca gtttgtactc
     4621 tttgttaaca tcaagtaatg gttttaaaca tgaatcaccg ggagtataaa tgatctttcc
     4681 tttatcaata agcgcagtca aaataattat ctgtggtttt tcttctaaaa tctgctggca
     4741 aaataattca tatctcttca cggcaggaaa ttgtgccatt tctttattat tcaggcagta
     4801 agaatcaata aaagttaatc cgcttaaaaa cggaatcaaa ccagaatctg ccaacaccac
     4861 tctatcttga ttcttggcat gagtatttaa ccattggaca acacttttcc gtaattgctc
     4921 tccatgcacc ggattttgac taaagtagcg ataagcatca agggacattt tgggtataaa
     4981 tagtagcgcc agaaaaaatg agctcacata caatgccgct ctaaagtaat tatcttgttt
     5041 tttgaaatag cttaataaaa gataactgat accattcaag gtcagaggta acataagagc
     5101 aaacgctggc aagaacaatc gattatcaaa agcgacaact ggatcggcat tcacaagcat
     5161 taataaataa ataataccgg gagaccataa aaaataatgc cttctgtcct gagcataaaa
     5221 acaggctggc aaagccagaa tagcaaatgg ccaaattaat ttgagataat tcaaatccaa
     5281 tgaataggat atgccattaa agcctttaca atatacagag ttaggaaaca ataacccgta
     5341 ataactccaa cgccaaagaa aataaggcag aaacaccaaa cctatggtca ccgcaaaaga
     5401 taccgcccct ctccatggct tcttgcctga taatttattc cagtcccaac cgattaaaag
     5461 aaaaaaaaga agcatcaggg ctggtgtttc aggcctggtc attcctgcga atgaaagata
     5521 caacccggac aaaacaaaac cacttgtctt gaattcacct cgaggagcgg gataaaaatg
     5581 atatcctaac ccacgaaaag caaaatagac tgaaccgcta atcagtgcct ggtaaacggt
     5641 cgtttcaagg ccgctaactg accaaataac ttgtcctttg tatagtaaaa gccaaatgca
     5701 ggggataaaa gactctcgtt tggtaaacca aaagcgggtg ataagaaaga taaaaagaca
     5761 cgtggcaagt aatccagcaa acccggcgat ctttagggta agaaccggat taatgtgcag
     5821 aataattgat attgccccca aagcaacaaa gctgaaattg gaatagcctt cgacaggctc
     5881 tgcgtttaaa ttccataata agccatttcc tgcagcccag tttttggcat accgcaaaga
     5941 aatatacatg tcatctatag tgaacagcca tatggcctgg atttgtaaaa ccagcaaata
     6001 agcaaacaaa aggataaaca ggctgtcata ccagcgccac ttagcactca tcaattcaat
     6061 gccatgaagg tcaggatagc gaagcaccat aaattgatcc gagataatcg atcacttaaa
     6121 aaaatattga ccggatcatc attctctaca tcctgagtag caagccaggc aaaacgccat
     6181 agtgcaatag cagcaaaagg taaggttaaa atgaaaaaaa acgattctgc tctggcataa
     6241 accgtgtaaa acagataagt aataaaacag gctattcctg taatgacgat aagtctttgc
     6301 agaaagactg gattatatcg gcgtagaacc ttacgagttg aatttttcaa tcccaactgc
     6361 atttccagtt gtcttttatt tagggcgata aataaactaa gcaaagtcgc tgcaacagtt
     6421 aaccaggctg atatagccaa atcaattcct attgttccgg ccaatactct aagcataaat
     6481 cctagggcaa tacaagcgac atcaaaaacc ggaatcaatt ttaaaacatg gttataagca
     6541 agattgatta tcaaataaat aattaaaatg accaccagtt tttttgaaat aagccaaccc
     6601 agcgcgattc cagcaaccat caataaaagc attacaatga tcgcatcaaa taaagtgacc
     6661 tgctcgctgg ctataggtct gttgcattta aagggatgta gactgtcttc cgttctatcc
     6721 tgaatatcat tataaatata aacggcactt gcaataagac aaaaagataa tgaagccatt
     6781 aatgccggaa acaaataccc
//
LOCUS       DAXLDT010000155         6800 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXLDT010000155 DAXLDT010000000
VERSION     DAXLDT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.155"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..497)
                     /locus_tag="ACF2SP_002548"
     CDS             complement(<1..497)
                     /locus_tag="ACF2SP_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFD2360322.1"
                     /translation="MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQL
                     NDLGIPIIRIPHQGYQLEKPLILLDSNEITKQLHSKGFSQPFNLHLFTSIDSTNRYLK
                     DLPSSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVIS
                     LATVLT"
     gene            complement(501..1238)
                     /locus_tag="ACF2SP_002549"
     CDS             complement(501..1238)
                     /locus_tag="ACF2SP_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFD2360323.1"
                     /translation="MIITGFTPLNTQNCILNESRIDLWQFSLDYELHSAYQLLNADER
                     SRADRFYFNRHKRRFTNARATLRIILARYLNTPPERLEFTYNAHGKPNVINSQKLQFN
                     ISHTGEMAILAVGKTYPIGVDIERYSARPYEGIGKNLFSEQEYQELKKAHQSLKPALF
                     FHIWAQKEAFIKASGLGLSYPTKEFHVPTSFPTKQLVDDPLHNMTWQLRSFMPEVCCS
                     GALCYHPSVREIRHGIVQLQQNISLKF"
     gene            1320..2273
                     /locus_tag="ACF2SP_002550"
     CDS             1320..2273
                     /locus_tag="ACF2SP_002550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFD2360324.1"
                     /translation="MTRQFLEFEQPIEELNQKIEALRMVGSDNEVNLSEEIARLEAKC
                     SELTENIFSRLEPWQIAQMARHPLRPQTTDYIERIFTDFQELHGDRSYSSAPAIIGGM
                     ARLNGEPVMVLGHQKGKRTKEKVYRNFGMARPEEYRKALRLMRMAEKFKMPVVTFIDT
                     AGAYPGIGAEERNQSEAIARNLFAMSRIKTPIVCIVTGEAGSGGALAIGVGDKIIMLQ
                     FSIYSVISPEGCASILWKDASKASEAARAMGITADRIFENELVDMVVPEPLGGAHRDV
                     DEMASRLKRLLTSELQALKKVPLDQLIELRYQKFMAMGACD"
     gene            2266..3561
                     /gene="tilS"
                     /locus_tag="ACF2SP_002551"
     CDS             2266..3561
                     /gene="tilS"
                     /locus_tag="ACF2SP_002551"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFD2360325.1"
                     /translation="MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLS
                     NQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDF
                     FSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
                     PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHC
                     QQAKANLEVLAIKDCPDLLNATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKT
                     FHRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTY
                     PDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVR
                     DRVPLVYINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS"
     gene            3911..4447
                     /locus_tag="ACF2SP_002552"
     CDS             3911..4447
                     /locus_tag="ACF2SP_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2360326.1"
                     /translation="MISESVIPLAYFITFTCYGTWLHGGKSTSVDRYNNIPGTEFIPY
                     DRRRLYQVKNLMLETPYSLDELRRHIVLQAICEVCRYRQWVLLAAHVRSNHVHLVIHA
                     QISPEHIMNTVKAYASRYLNYTRLDGDRKNRWTRHGSTRYLWNEEEIEATIQYVVYEQ
                     GLPMAVFENKDRVFGVEV"
     gene            4786..5952
                     /locus_tag="ACF2SP_002553"
     CDS             4786..5952
                     /locus_tag="ACF2SP_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HFD2360327.1"
                     /translation="MSDIHCEKSYRSKLLKQGLLGVLILCLVFLFLESFSRWIAATST
                     APFGESIRNDQKMIVSDSIRQGIVAIGDSIIARSFYPELFSSLLAKNFSKPYEVYNIG
                     VEGSGIIHHRAHLRYLLKRNVKPDFVIMNVSVHNLNLNYFAHDSNEHIIEQRYEDHNS
                     LDYYNRCFTVNKQTLSSTLYCKIAKYSYFFRLVHYYHQQVNNSLQSLLHTQRLRTENL
                     IIKYPAISRQGFSPIFGSLAEDTISTPRMFEFLRHEYRSAFANFKLTTEVLDSIIDDL
                     NNNSIKVILVLAPVYHPVTRKVYDEFNLPSNGAIVSTLENYALKKNIAFINLFEECQD
                     PVYFQDPVHPNIYGAVKITTLLSQKIINKQFVGPDGYLDKELFQYLKSNNENKA"
     gene            5949..>6800
                     /locus_tag="ACF2SP_002554"
     CDS             5949..>6800
                     /locus_tag="ACF2SP_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFD2360328.1"
                     /translation="MNFVTFQFVVFLVITVIVFMMMSPYYRKYFILLCSYFFYGTWSV
                     PLISVILFSTSVDYYLGKKIHESHSQTHKKLYLTLSILVNCSILVYFKYMNFFISSID
                     AIGFSLGATQLLLNPMHIILPLGVSFYTFEAISYIVDIYRGEKPASSWLNYNFYIMYF
                     PHLISGPIVRYKELHAQYESKIELPETSRLKEGFQLLLYGFIFKLVIADSMASISDPL
                     FDKYQTIGHLESWLASIAFTIQIYFDFLGYTHIARGVSLFFNIKLPVNFNHPYNARNI
                     SEFWQRWH"
BASE COUNT         1987 a         1218 c         1383 g         2212 t
ORIGIN      
        1 gtgagaactg ttgctaaact tatgactaaa ctgagacccg atagcttaga tatgtcacaa
       61 ttaaaactcc accgggtaga gcagtaaata ttctctccaa aaggagagtg ccagtgtcgt
      121 ccaaagcgcc ctctcccttg agtttgcatt tcagtgcagc aaatttcgac ggctgagctt
      181 gaaggcagat ctttcaggta gcggttggtt gagtcaatcg atgtaaataa atgcaggtta
      241 aacggttggc taaatccttt gctgtgtaat tgcttggtaa tttcattgga gtctagcagg
      301 attaaaggct tttccaattg gtatccttga tgtggtatgc ggataatagg tatgcctaaa
      361 tcattaagct gattgatttg tttccatact gctgagcgac tgatttttaa agcgtttcct
      421 aattcactcc cactatgaca tgccccatcg cccagtattt gcattaacgt atgttgagaa
      481 gtattaaatt gaaacatgat ttaaaatttt aaactaatat tttgttgaag ctggacgatc
      541 ccatgacgaa tttctctaac agaagggtga tagcataaag cgccactgca gcatacttcc
      601 ggcatgaatg agcgtagttg ccaggtcatg ttatgcaagg gatcgtcgac caattgtttt
      661 gttgggaaac tggtagggac atgaaattct ttggttggat aggagagacc taaaccactc
      721 gctttgatga acgcttcctt ttgagcccat atatgaaaaa ataaggctgg cttaagggac
      781 tgatgtgctt ttttcagttc ctggtattct tgctcggaaa acaaattttt accaatccct
      841 tcataaggcc gggctgaata tctttcaata tccactccaa tcggataggt tttacccact
      901 gccaatatgg ccatttcacc ggtatgactg atattaaatt gcaatttttg cgaattaatc
      961 acattgggct tgccatgggc attgtaagta aattccaatc gttcaggggg cgtattaagg
     1021 tatcgggcca ggataattct taaagttgct ctggcgttgg taaagcgtct tttatggcga
     1081 ttaaaataaa agcgatcagc acgggagcgt tcatcggcat tcaataactg atacgcacta
     1141 tgcaattcat agtctaatga gaactgccaa agatcaatac gtgactcatt taaaatgcag
     1201 ttttgtgtgt ttaatggagt aaatcctgta attatcatct gttgtatctg aaatctggca
     1261 tcgattaacg gttaagagta tcatatacca aagatgttta attcaccaaa gagtaatcaa
     1321 tgacccgaca atttttggaa tttgaacaac ctattgaaga gttgaatcaa aaaattgaag
     1381 cactgcgtat ggttggcagt gataatgaag ttaatttaag tgaagagata gctcgacttg
     1441 aagcgaaatg ctccgagtta acagagaata tattctccag gcttgaacca tggcaaattg
     1501 ctcagatggc cagacatcct ttaaggccgc agactactga ctatattgag cgaattttta
     1561 ctgattttca ggagttgcat ggtgatcgca gttattcttc agcccctgca attatcggag
     1621 gcatggcgcg attaaacggg gagcctgtca tggtgcttgg tcaccaaaaa ggtaaacgta
     1681 ccaaggaaaa agtatacaga aactttggta tggcaagacc tgaagaatac aggaaagcac
     1741 tgcgtttgat gagaatggct gaaaaattca aaatgcctgt agtcacattt attgatacgg
     1801 ctggagctta tcctggcata ggtgctgaag aacgtaacca atcagaagcc attgcacgca
     1861 atctttttgc tatgtctcgc attaaaacac caattgtctg tattgttaca ggagaagccg
     1921 gttcaggtgg tgccttggcg attggggtcg gtgataagat tattatgctg caattcagca
     1981 tttattcagt catttctcct gaggggtgcg cctcaatcct ttggaaagac gcctctaaag
     2041 cgagtgaagc cgcgcgtgct atgggaatta ctgcagacag aatattcgaa aatgaattgg
     2101 ttgatatggt agtccctgag cctttggggg gcgcacaccg agacgttgat gagatggcaa
     2161 gtcgcttgaa gagattatta acgagtgaac tccaggcttt gaaaaaggtg cctctggatc
     2221 aattaattga gttaaggtat caaaagttta tggcaatggg tgcttgtgac taggccttta
     2281 ctaagtcccg aatgggtagc tcgccttaaa caattcaaga aattgatagt agggttcagc
     2341 ggtggtctgg actctacagt gctcctgcat gtccttgcct caactcctcc cttgtctaat
     2401 caacttttgg ctgtccatat taaccatggc attagtggaa attctctgaa ctggcaaagg
     2461 cattgtgagc aattgtgcct ggatttaggt attgctttta ttgccaaagc gattgaattt
     2521 gatcgttctg caaatgttga agaagcagca cgcaatgcga gatatgattt tttttcctcc
     2581 ttattagagg ataatgattg tttggttttg gggcatcatc ttgatgatca ggctgaaaca
     2641 gttcttttgc aattgtttcg cggtgcagga gttgatggct tggcagctat gcaggaatgc
     2701 agcaatttgg gtgctgggca attggcaaga ccatttttga cctgccccag agtggaatta
     2761 gagcattatg ctcgtattca tgaattgaaa tggatagaag acgaaagcaa tcaagataca
     2821 aaatattctc gcaattatct gcgtcatcgc gtaatgccat tattgcttga aaagtggccg
     2881 ggagtagcag gtaatatttc ccgagctgcg actcattgtc agcaggcaaa agcgaatctt
     2941 gaggtattgg ctataaagga ttgtcctgat ttgttaaacg ctactgatca tttgctgatt
     3001 gaacccataa aaggacttga atttgagcgg attacaaatg ttttaaagtt ttggcttaaa
     3061 aaaaatagaa ttcaattgcc ttcgtccaag acgtttcatc gtatcattca tgaaataatt
     3121 tttgcaaagc tcgatgctat gccaacagtg agttggaatc aaatacaagt acgtcgtttc
     3181 caacaacatc tttatttgct taaggcagac cagatcaatt taccgaaggc tattaaatgg
     3241 caacaatttc cagcttcttt aacttatcca gacgctgata ttaagttgtc tgcagtaaag
     3301 gcccaaaagg gattaatgat cccgaaagat gcgcaaatag aaatacgatt cagaaagggt
     3361 ggtgaagaga tttattggca tggtcaaacc aaacatctaa aaaaactatt tcaggaatgg
     3421 caaattcccc catgggtaag ggatagagtt ccattggttt acattaatga tcaattggct
     3481 tgtgtagtag gttatgcagt gagcgatttg ttttttacta ctaatccact ggaagcttgg
     3541 tctatagtta ataattctta atataacaat ctccgctccc ttacggtcgc ggctcggttt
     3601 tgactataat tccatttgca ctcccttatg gtcgtagctc ggttttgact ataattccat
     3661 ttgcactccc ttatggtcgc ggctcggttt tagccataat tccatttgca ctcccttatg
     3721 gtcgtggctc ggttttagcc ataattccat ttaataatct gattaatcat ctgagccgcg
     3781 accgtgaggg agcggaaaac tggttattgt actaattttc cacagaaaaa tttacttcaa
     3841 aaataattag ttagtgtgat aaccttttct tacataaaca agtcctaaac caaatggaaa
     3901 tggtgaaata atgatatctg aatcagtcat tccactcgcc tattttatta cctttacctg
     3961 ttatggaaca tggttacatg gaggtaaatc aacttcagtt gatcgctaca ataatatccc
     4021 aggaactgaa tttatccctt atgatcgaag gcggttatat caggtaaaaa acctcatgct
     4081 ggaaacacct tattctctgg atgaattaag gcgtcacatt gttttgcaag caatttgtga
     4141 agtgtgcaga tatcgtcaat gggtattact ggctgctcat gtaagatcaa atcatgtaca
     4201 tcttgttatc cacgcccaaa tttctcccga acacattatg aatacagtaa aagcatatgc
     4261 ttcacgttat ttaaattata caaggctgga tggtgataga aagaacagat ggactcgcca
     4321 tggaagtact cgttatcttt ggaatgagga agaaattgaa gcaacgattc aatacgttgt
     4381 ttatgaacaa gggttaccaa tggctgtttt tgaaaataaa gacagagtgt ttggtgttga
     4441 agtttgatct atccgctctc ggttttagtt ataattccat ttaatccact cccttacggt
     4501 cgtggctcgg ttttagccat aattctattt aatccactcc cttacggtcg tggctcagtt
     4561 ttagccatag ttctatttaa tccgctctta cggtcgcggc tcggttttag ccataattcc
     4621 atttaattat ctaattaatc atccgagccg cgactgtgag ggagcggatt acgtttaaac
     4681 taaattattt cttcagtttt tttatagctt ttaatactat acatcattat caaatatgtt
     4741 tattctgcgc aaattcgaat attgtaaaat taatttctgt tatttatgtc tgatatccat
     4801 tgcgaaaaaa gctacagatc aaaacttctg aaacagggct tgcttggtgt attaatttta
     4861 tgcctggttt ttttgtttct tgaatctttt tcaagatgga tagcagctac aagtacagca
     4921 ccttttggtg aaagtatcag gaatgatcag aaaatgattg tgagtgactc catcaggcaa
     4981 ggtattgttg ctattgggga ttccataatt gccagaagtt tttatccgga acttttttct
     5041 tctttattag ctaaaaattt ttcaaagcct tatgaggtgt ataacatagg agtggaaggt
     5101 tcaggaatta tccatcatcg agcacactta agatatttat tgaaaaggaa tgtcaaacca
     5161 gattttgtga ttatgaatgt ttctgtccat aatcttaatt tgaattattt tgcccatgat
     5221 agtaatgaac atatcataga gcagagatat gaagatcata actccttgga ttattataac
     5281 cgttgtttta cagtaaataa gcaaacactt tcatcaacgc tttattgcaa gatagcgaag
     5341 tattcttatt tttttcgttt agtgcattat taccatcaac aagttaacaa tagtttgcaa
     5401 tctttgctgc atacacaaag attgagaact gagaatttga taatcaaata tccagcaatc
     5461 tctagacaag gtttttcacc aatttttggc agtcttgctg aggatacaat aagcacgcct
     5521 agaatgtttg aatttttgag acatgaatat aggagtgcat ttgcaaattt taagttaact
     5581 actgaagttt tagacagcat tattgatgat ctgaataaca attcaattaa agtaatttta
     5641 gtcttggcac cagtttacca tccagtaact cgtaaggttt atgacgaatt caacttgcca
     5701 tctaatggcg ctatagtcag tacgcttgaa aattatgctt taaagaaaaa tattgctttt
     5761 ataaacttat tcgaggaatg tcaggatcct gtttatttcc aggatccagt acatcctaat
     5821 atctatggtg ctgtaaagat aactacattg ttatcgcaaa aaattatcaa caaacaattt
     5881 gtagggcctg acgggtattt ggataaagaa ttgtttcaat acctaaaaag caataatgag
     5941 aacaaagcat gaattttgtc acttttcagt tcgttgtttt tctagttatt acggtcattg
     6001 tctttatgat gatgtctccc tattatcgga aatattttat tttgctttgc agctattttt
     6061 tttacggtac ctggagcgtt ccattaatca gtgtcatctt attttcaacc tcggttgatt
     6121 actatctcgg aaaaaaaatc catgagagtc attcccaaac tcataaaaaa ctctatttga
     6181 ctttatccat tttggttaat tgctccattc tggtttattt caaatacatg aattttttta
     6241 tttccagcat tgatgctatt ggctttagtt tgggagccac ccaacttttg ttaaatccta
     6301 tgcatattat tttgcctctc ggcgtttctt tttacacttt tgaggcgatc agttatatcg
     6361 tagacatcta tcgaggggaa aagccggcta gtagctggtt aaactataat ttttatatta
     6421 tgtatttccc gcatctgatt tcaggaccga ttgtccgtta taaagaactg catgctcaat
     6481 atgaaagtaa aattgaatta ccggaaacct cacgtttaaa agaggggttt cagttattac
     6541 tttatggctt tattttcaaa ttggtcatag ccgattcaat ggcttcaatc tcagatccct
     6601 tgtttgataa gtatcagaca attggccatc tggaatcctg gttggccagt attgcattta
     6661 ccatacagat ttattttgat ttccttggat atacccatat tgcgcggggt gtatcattat
     6721 tttttaatat taaattacct gttaatttta atcatcctta taatgcgcgc aatatcagtg
     6781 aattctggca gcgttggcat
//
LOCUS       DAXLDT010000156         6778 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXLDT010000156 DAXLDT010000000
VERSION     DAXLDT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.156"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..254
                     /locus_tag="ACF2SP_002555"
     CDS             <1..254
                     /locus_tag="ACF2SP_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HFD2360329.1"
                     /translation="NIPRSLEKACNKNILPFKFNVDTATFMVEIPNIMASKEKRSLSF
                     YWQEKLFAFLMRNYSANLNIEFYKLPYNRTIAIGTYCIL"
     gene            372..1310
                     /locus_tag="ACF2SP_002556"
     CDS             372..1310
                     /locus_tag="ACF2SP_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFD2360330.1"
                     /translation="MWFNNALMFQYKLDGGCDLNRSLTEEALKPCPPHARFTYGWVPA
                     FEQEMVHEVAGSTLICLGKEERILPRGVINKMLKEKIQQIEAAQGRPVKRAEKAQMAE
                     DLEFELLPKSFCVQKKLLAILDSVSQRLIINTSSSNQASQLTSFLRKSIPGIVIEPLN
                     LTENLALRFAEWIHSPATLPDDFQLASDCLLFSLEDEKKRVHCKGYELPAEEILTLLA
                     QGMGTAEISLIWKERIQFTLTQDFTFKKLKSLDYLIDDFNEIRQLEEEYQRQDAALTL
                     LSGELRELINGLMKIFVNHAEMKSAQIEKIAEDCTI"
     gene            complement(1458..2231)
                     /locus_tag="ACF2SP_002557"
     CDS             complement(1458..2231)
                     /locus_tag="ACF2SP_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFD2360331.1"
                     /translation="MKRIHVLFLIGLVVGISSCTKWGKDDEDNNPYKGMTAKQLYTAA
                     QTSLKKEEYATAAKQLEAMESMYPFSDYTESSQMQLIYAYYKDEDYPSAAATAERFIH
                     LYPRAKNVDYAYYMRGLANFQQTRGVFAKMLPMDESWRDPGTQTQALVDFATLIQKFP
                     DSKYKANALQRMIYLRNMFAQHELNVSLYYFKRKMYVAAIERASYLVKNYPQAPSAQK
                     ALVVMYEANKALGLNKAAEDAMAVYKATYHTSNMVRINS"
     gene            2397..2484
                     /locus_tag="ACF2SP_002558"
     tRNA            2397..2484
                     /locus_tag="ACF2SP_002558"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2431..2433,aa:Ser,seq:tga)
     gene            complement(2615..5002)
                     /locus_tag="ACF2SP_002559"
     CDS             complement(2615..5002)
                     /locus_tag="ACF2SP_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD2360332.1"
                     /translation="MLTDAQIQQAINNLNILIAHYYSPISSFSFDNERVTKNWFFKSE
                     SIHATALSNYLKKIIDEVSSDKVDKTNSLYASLFITLQNFLHIQPNRVIEGLNFNLEN
                     SQCLKRKNLIIKRLEHFHIENQLRDDLKRHLLPEIQYDTCTGHYTSPDDDKAHQHGSE
                     AQRIFFSTQWERLKALCNWLLAKIGIHIFETARYKEHDYLDSEVYANDPAIEPGVSPQ
                     QSATHYWIGHASNLMTIPAHDSPLHVVTDPVEGDLAPFLYPRMTKEGSLIDGTGSKKL
                     PKIDIVVISHNHHDHVSQSTLKRLLKQQPKMVVPEGDESFFLGMGFTDVVGLKWWEQA
                     KITDFQGNELLRITAVPARHWSGRSLTDAHCSAFNGYVLHANQLDGDIYFAGDTALMD
                     DMLSQPIFEKFNIVTSIQPGGPDERREDMESTHQSSADAILMHFKILAAQYQKMKEKN
                     ESLSLDEFLMENQHIKTLYNHTATFKLGNLRLKDTYYSYQRMVAAFREDAQWRADHLP
                     KHEQKVYDHIQLLISNMVFKNNQRLSNRQIADLILGKVVIPKIGQRQPLYFKEKSIKT
                     EAFQYRNLITNRRALLEFDMLLQESIAESNEFFNIKQLILNLLRAYQNPWHAFFSRTH
                     LGVGNYEQDIRNCQSNSDLLAVLNHMERNLGKRNPHGHMQSLIYYAKWVIDFSNKYQH
                     ETLMKFKEYFACQQIRKLADQEIHHAGLIMGFGDRQAKQQRFKVLSNELADLPVDVTE
                     YRKAIDNWLATRVNDGQSTQQLLSENRSSVFAKKVTRGESTVLHIKTTLDLAVSS"
     gene            complement(5094..>6778)
                     /gene="ravS"
                     /locus_tag="ACF2SP_002560"
     CDS             complement(5094..>6778)
                     /gene="ravS"
                     /locus_tag="ACF2SP_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavS"
                     /protein_id="HFD2360333.1"
                     /translation="ERTCYVDFDGLTVAQLKSDWLTVNPQGDLALSEGVYPSFSQGDN
                     TGAVIGEILQTKHFSLPLSEEDPKAFMEVTRNTTRICFSYKNSSGQPSGFSLVAAPNG
                     DSEGKHKGWIISVIQNTVGNYKDRKVSVLASPNYVRSEAQSTVDMDTPDAIERELKRV
                     INNDKVASLIKGVFKEDGTLNGDYIRQLASRIKGNRNIDDRDRKSAQFNILIELAEQL
                     DDEKLRHYINSIKADMAADIEFFKDGIYEQHLTELSNKISLSLSKMPSVNKKTTEIGR
                     ILNNISERYAQIKMIEDAQRKNPELAHIFKAMAQKIKSDFYDIKFDGTHQDNRNILLL
                     SQFEVASVEQKIRNYYQTNPLVKQYFIDKDKANHHLQVDLSRLSPPKNEGFFSRNKDS
                     FGFSIFMGLIVGVSAALFLTGVLAPIGVALIAVGAALITTALVGSIDIYVNEKEYAKE
                     TSHYSNEVKCLRSAHREHLVRLEKNLHEQINLSDFLTPDKPEFLSGIKVNKKKPLTPE
                     SVAHALWLQDKSARDTSVAKNRFSIWAAKQENLDFGIEEVESVIKQRYVMKS"
BASE COUNT         2036 a         1325 c         1215 g         2202 t
ORIGIN      
        1 ttaatatccc aagaagtctg gaaaaagctt gcaataaaaa tatacttcct tttaagttca
       61 atgttgacac agcaacattt atggttgaaa tacccaatat tatggcttcg aaagaaaaaa
      121 gatcactaag tttttattgg caagaaaaac tgtttgcttt cttgatgcgc aattactcag
      181 cgaatttaaa tattgaattc tataaattac cttacaatag aactatagca ataggtacct
      241 attgtatttt atgagttttg gccagggtct gttgacagtt tgctctcttg cgtcgaaatg
      301 gcaacagccc ctaatcatcg cagtaaaatg ctatccgtga taacatccca aatttatttt
      361 aaggactgat tatgtggttc aataatgcac tgatgtttca atacaaacta gacggtggat
      421 gtgacttaaa ccgttctttg actgaagaag cgcttaagcc ctgccctccc catgctcgct
      481 ttacttatgg ctgggttcct gcgtttgagc aggaaatggt gcatgaagtg gctggtagca
      541 cgcttatttg cctgggtaaa gaagagcgaa tcctccctag aggagtcatt aataaaatgc
      601 tcaaggaaaa aattcagcaa atagaagcag cccagggtcg accggtaaaa cgcgcagaaa
      661 aggctcaaat ggctgaggat cttgaatttg aactattgcc taaatccttc tgtgttcaaa
      721 aaaaactttt agcgattctt gatagtgtca gtcagagatt aattattaat acttcaagca
      781 gcaatcaggc gtctcagttg acttcttttt tgcgtaaatc aatccctggt attgtgatag
      841 aaccattaaa ccttaccgaa aatcttgctt tacgattcgc agaatggatc cattctccag
      901 caactctccc tgatgacttt caattagcct ccgattgttt actcttttct cttgaagatg
      961 aaaaaaaacg agtccattgc aaaggctacg aattacctgc cgaagaaatc ctgactctat
     1021 tggctcaagg tatggggact gcagaaatct cattaatttg gaaagagcgg attcagttca
     1081 ccttgacgca ggattttact tttaaaaaac tgaaaagcct tgattatctg atcgacgatt
     1141 ttaatgagat cagacaattg gaagaagagt atcaaagaca agatgccgcg ttgaccttat
     1201 taagtgggga gttaagagaa cttattaacg gattaatgaa gatatttgtt aatcatgcag
     1261 aaatgaaatc ggctcaaatt gaaaaaattg cagaagattg cacaatataa agtcgtatca
     1321 ttttgacctg taacctatcg agggaacttc tttgctaaca gagctccctc tcatcttgac
     1381 tcaagagaaa acgagtggat tacacatcac ttgctgaacc cagctgacaa aataaactct
     1441 ataaattttt gtactcatca agaattaatt ctgaccatat tggatgtatg ataagtagcc
     1501 ttgtaaacag ccatagcatc ctccgctgcc ttattaagcc ctaatgcttt attggcttcg
     1561 tacattacca ctaaagcctt ttgagcgctg ggagcttgag gatagttttt cactagataa
     1621 ctggcccgtt caatggcagc cacatacatt ttacgtttga agtagtaaag agagacattg
     1681 agttcgtgct gagcaaacat atttcgtaga taaatcatac gttgcaaagc attggcttta
     1741 tatttgctgt caggaaattt ctgaattaaa gtagcaaagt caaccagtgc ttgagtttgt
     1801 gttccagggt ccctccagga ttcatccata ggaagcattt tggcaaatac gcctcgtgtt
     1861 tgctggaagt tagccaaacc tctcatataa taggcataat caacgttttt tgcgcgtgga
     1921 tacagatgaa taaagcgctc agccgttgcc gcagcagaag gataatcttc gtctttataa
     1981 taggcataaa taagctgcat ttgagagctt tcagtatagt cactaaaagg atacatagac
     2041 tccatggctt ccaactgttt ggcagcagta gcatattcct ctttttttaa agaggtttgt
     2101 gccgcagtgt agagctgctt agctgtcatg cctttatatg ggttattatc ctcatcatct
     2161 tttccccatt tagtacacga tgagattcca acaactaacc caataagaaa taacacatga
     2221 attcgtttca taaagcacac ctatcaatta tatctgctgt atttataagt acagcaactg
     2281 gaaaatttgg gacattatac ctaggaccag ttaatttgcg aacaacctat tattctttta
     2341 tttatctatg aaaaaacatt tgcaaaatga tatgcgttca tatagtattc tcgaccggag
     2401 agatggcaga gcggtcgatt gcggcggtct tgaaaaccgt tgaagggcaa ccttcccagg
     2461 gttcgaatcc ctgtctctcc gccagacaac attaccacgg tatattttcc caagacaacc
     2521 aatttcactt tagcgttatt ctattcttca agcacaaaaa ttaagtgaat gacatctaac
     2581 tcattaatac aaaagactaa agtcattgta tctattaact tgatactgcc agatcaagag
     2641 ttgttttgat atggagcacg gtgctttctc cacgggttac tttttttgca aaaacagatg
     2701 agcgattttc actaagcaat tgttgggtag attgtccatc attaactcgt gtggcaagcc
     2761 aattatcgat ggcttttcta tattctgtaa catccacagg caaatcagcc aattcatttg
     2821 agagtacttt aaaccgctgt tgcttcgctt ggcgatctcc gaaacccatt ataaggccag
     2881 catgatgaat ctcttgatca gccaattttc ttatctgctg gcaagcaaaa tattctttaa
     2941 atttcattag agtttcatgt tgatatttat tggaaaaatc aattacccat ttagcataat
     3001 aaatcaaact ctgcatgtgg ccatgtggat ttcttttgcc cagatttctt tccatatggt
     3061 ttaatacagc gagtaaatcg ctgttactct ggcaattgcg gatatcttgc tcgtaattac
     3121 ctactccaag atgcgttcgg gaaaaaaagg catgccaggg attttgatag gctcgcaaca
     3181 gatttaaaat aagttgtttg atattaaaga attcattcga ctcagcaatg gattcttgca
     3241 gcaacatgtc aaattcgagt aaagcacgac gattagtaat cagattacga tattgaaatg
     3301 cttctgtctt tatcgatttt tctttaaaat aaagtggctg tctttgacca attttgggaa
     3361 taactacctt gcccagaatc agatcggcaa tttgccgatt actaagccgt tgattatttt
     3421 tgaaaaccat attgcttatc aaaagttgaa tatgatcgta aaccttttgc tcatgctttg
     3481 gtaaatgatc agctctccat tgagcatctt ccctaaatgc agcaaccatt ctttgataac
     3541 tgtaataggt atctttcaaa cgtaaatttc ctaatttgaa agtagcagta tggttataaa
     3601 gagttttaat atgttgattt tccattaaaa actcatcaag agataaactc tcattcttct
     3661 ctttcatttt ttgatattgt gcagccaata ttttaaaatg catcagaatt gcatctgcag
     3721 aagattgatg agtagactcc atatcttcac gccgctcatc tggaccaccg ggttgaatag
     3781 aggtaacgat attgaatttt tcaaaaatgg gctgtgataa catgtcatcc atcaacgcgg
     3841 tatccccagc aaaataaata tctccatcta attgatttgc atgcagcaca tagccattaa
     3901 atgctgagca atgagcatcg gtcaacgatc gacccgacca atgcctggca ggcactgcag
     3961 ttatccttaa taattcattc ccttggaaat ctgtaatctt tgcttgttcc caccatttca
     4021 accctacaac atcggtaaat cccatgccta aaaagaatga ttcgtcaccc tctggaacta
     4081 ccatctttgg ttgctgtttt aataggcgct tcaatgtgga ctggcttaca tgatcatggt
     4141 gattgtgaga aatcactact atatcaattt tgggtaattt tttactacca gttccatcaa
     4201 ttaaactccc ttcttttgtc atacgcgggt atagaaatgg agccaaatca ccttcaacag
     4261 gatcagtgac tacatgcagt ggagaatcat gagccggtat ggtcattaga tttgatgcat
     4321 ggccaatcca gtaatgagtg gctgattgtt gtgggcttac ccctggttca atcgctggat
     4381 cattggcata gacctcacta tccaaatagt catgttcttt ataacgtgcg gtttcaaaaa
     4441 tatgaatacc tattttagcc aataaccaat tgcataaggc ttttaatctt tcccattgag
     4501 tagagaagaa aattcgctgg gcttctgagc catgttgatg cgctttatca tcgtctggcg
     4561 aggtataatg cccagtgcac gtatcatatt gaatctcagg aagaagatgc cgtttcagat
     4621 catccctgag ttgattctcg atatgaaaat gttccagtct cttgataatc aagtttttgc
     4681 gtttcaggca ctgactattc tctaaattaa aattcaagcc ttcaattacc cggttcggtt
     4741 gtatatgaag gaaattttgt aaggtaataa acaaactagc atataaacta ttagtcttat
     4801 ctactttgtc gcttgaaact tcatcgataa ttttctttaa ataattactc agggcagtag
     4861 catgtattga ttcagatttg aaaaaccagt tctttgtaac tcgctcattg tcaaaagaaa
     4921 agctggatat tggcgagtaa tagtgtgcaa taaggatatt aagattatta atagcttgtt
     4981 gtatttgagc atcagttaac atattttctt accgtattat ccaatcagct gattcgtttg
     5041 ctatattttg ccaagagtta taaatatctg cttcattgaa ttctcaatat tttttatgat
     5101 ttcatgacat atctttgttt gattacgctt tccacttcct ctataccaaa atccaggttt
     5161 tcctgcttgg cagcccatat cgaaaatcta ttttttgcta ctgaagtatc tcgagcactt
     5221 ttatcctgta gccataaagc atgggctacg ctctccggag tcagcggttt tttcttattg
     5281 actttgatgc ctgataaaaa ctccggttta tcaggagtaa ggaaatcaga aaggttgatt
     5341 tgctcatgta aatttttttc taaacgaacc aaatgctccc tatgagcact ccttaagcat
     5401 tttacctcat tagaataatg cgatgtttct ttagcgtact ctttctcatt gacataaata
     5461 tcgattgacc ctaccagtgc tgttgtaatc aaggctgctc caacagcaat aagagctact
     5521 ccgatggggg caagaacgcc agtcaaaaat aatgccgcac tgactcctac gattagaccc
     5581 ataaaaatac taaatccaaa tgaatcttta tttctactga aaaaaccctc atttttggga
     5641 ggagataacc ggcttagatc aacttgtaaa tgatgatttg ctttatcttt atctataaaa
     5701 tattgtttta ccagaggatt ggtttgataa tagtttctaa tcttttgttc aacacttgcg
     5761 acctcaaatt gacttaagag caaaatattt cgattatcct gatgtgttcc atcaaatttt
     5821 atatcataga aatcggattt tattttttga gccattgctt taaaaatatg ggctaactcc
     5881 gggttctttc tctgtgcatc ctcaatcatt ttaatctgtg cataacgctc agaaatatta
     5941 tttaatattc tgcctatttc agtagttttt ttatttacag aaggcatctt acttaacgat
     6001 aaggaaatct tgttggataa ctcagttaga tgctgctcat aaattccatc tttgaaaaac
     6061 tcaatatctg cagccatatc agcttttatt gaattgatat agtgtcttaa tttttcatca
     6121 tccaattgtt ctgccaattc gatgagaata ttgaattgtg cagattttcg gtcacgatcg
     6181 tctatatttc gattcccttt gatccgtgaa gccaattgtc gaatataatc cccattgaga
     6241 gttccatctt ccttaaaaac gccttttatt aaactggcga ctttgtcatt attaatgact
     6301 ctctttaatt ctctttcaat agcatcagga gtgtccatat cgactgttga ttgagcttca
     6361 gaccttacat aatttggaga agcaagaact gatactttcc tgtccttgta attcccaacg
     6421 gtattttgga taactgaaat aatccagcct ttatgttttc cttcactatc accatttgga
     6481 gcagcaacca gggaaaatcc tgagggttgt ccagaagaat ttttataaga aaaacaaatt
     6541 ctcgttgtat ttcttgttac ttccatgaat gctttagggt cttcttctga caaaggtaac
     6601 gaaaaatgtt tggtttgaag aatttctcct attaccgcac cggtattgtc tccctgagaa
     6661 aaggatggat aaacaccttc tgatagcgcc aaatcacctt gtggattgac agttaaccaa
     6721 tcagatttta attgcgcaac agtaagtcca tcaaaatcca cgtaacaggt tctttcaa
//
LOCUS       DAXLDT010000157         6667 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXLDT010000157 DAXLDT010000000
VERSION     DAXLDT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.157"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            224..2572
                     /locus_tag="ACF2SP_002561"
     CDS             224..2572
                     /locus_tag="ACF2SP_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HFD2360334.1"
                     /translation="MRYSLLLPGILTVIATSVSANTSLKPSIAQPATCVNSLFTATGN
                     QHWKSIILKLTNNCNQAVDFQNSTVSFQTAEALNTSFWGDFSPLSYPDNALNISSQPQ
                     SGGNYLATFNLHFPSYPGANSKLPVGSSISIKYGATTDSHIEGTANVYLSTTVEAGSI
                     QLINAATKPTNVSQGYALVHVTMNGQSISDVQLPWQTSKTLSGLAAGNYAISAETVTD
                     SNGNLYLGQANPSMVSVIANETTSSTINYARVQETGKIKIHVQNLPGELNNYNENPTV
                     LLTQSQSGNSRSQPAVWGNTTTVAELKEGSSYQFSTPAIQYNDYNCWPTFTPFSLVAS
                     ATNVPTTNLTYQCIQVVKDTVTLSVSGAPASLASLKIILTPNDGSQTVEQTVDLANGV
                     GSSAISLTDGVIYTLSSEAVSGYTVQFSPQPLTATANATVNITLSQVTSAKGRIIGYI
                     PGWKTPPTAQELANAGYTHVMIAFGVFSTNTPGVIVPAFETVTKEYIQSLHQAGIKVI
                     LSLGGALTSIPNTTVDFHQVLAASSSPDVFKQTFINSLKGLISQYGFDGFDMDIEHGI
                     NAGGSFSQPQGDIAVLASIINTMYSQNPSLLITLTPQVANIAATSGFDQTWGNYASLT
                     MQTHQSLAWVGIQLYNTGCAFGIDQVCYGPTPTDIPDFSVAMATDLLENWPATVNGRP
                     TGFQPYISYLRPSQIVIGYPSPNAGGGSDGSPVTPTTTIKRAIQCLKTAIASNTSCGA
                     YVPPRAYGNIGGVFNWEVTYDKNNQFKFAKELKNCVINGVCE"
     gene            complement(2681..3154)
                     /locus_tag="ACF2SP_002562"
     CDS             complement(2681..3154)
                     /locus_tag="ACF2SP_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFD2360335.1"
                     /translation="MAIYKFEISTMQNGNIQISAKHKSTGEIHGYVQLASSARLESRN
                     LLEIDQLYVDNNARGHGLGSELASRAIKYALKMDKGIHMMIQPGSDVFWKKYFNSRFD
                     GKRITLFGGETLNTNLADGKVFEISKESLVANYKFPQDRHLRDRDMEKICAKLSH"
     gene            complement(3246..4361)
                     /locus_tag="ACF2SP_002563"
     CDS             complement(3246..4361)
                     /locus_tag="ACF2SP_002563"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFD2360336.1"
                     /translation="MSRYVCKWKLIYTFLIFIVFNNCPLAFASPSLTKQLHTILKDNL
                     ADSLTPGAVLLISSPDLGTISVAAGLADKENDISMNITDSFRLASMSKTFLAVTVLKL
                     VADKQLNLDDHIADLLPDSIDIERIPNGDEVTVQQLLQMRSGIPNYTDYDSYSDLIDT
                     MADKEWTPEECIKLVYDQKPDFKPGTAYEYSNTNYLLLQLIIENLTGESYAESIKKYI
                     LKPLRLKNTYIEKQEFDDDHHYLSTHGYTLEDGKVVDITDYDDGFGLADGGIISTAED
                     INIFVQALLKDKILLSPNYLTMMLTFPDDYGFGIGREEINGEIAWSHNGASSGYQGQY
                     YYFPDRQLTVVILTNYFDSDIIEDIASQTLSAIDNSE"
     gene            4629..5693
                     /locus_tag="ACF2SP_002564"
     CDS             4629..5693
                     /locus_tag="ACF2SP_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFD2360337.1"
                     /translation="MVNKICLSFALLISVPSILLAEDKLIFAVDIIRHGDRTPIVALP
                     TVNYQWQEGLGQLTAEGMQQEYKMGVVFRKKYIEESHLLPEHYEYGTIYVRSTDYART
                     LMSAQSLLMGLYPPGTGPAIPAGTSALPHAFQPIPVFSAPSKYDEVIIQQVDRKEREK
                     LMEQYVFSTREWQQKNNELKDKYPLWSRLTGINIDNLEDLETVGHTLYIHQIHNAPMP
                     EGLASNDIETIINSAEWAFMAQEKPQQIANAYSSKLMTNIADYLNSGSMKKSKLKYVL
                     LSAHDTTIASVLSFLGAPLEKSPPYASNVNFSLYDNGANYYKVKITYNGNPVLIPACG
                     GSVCELQQLINLVHDSKNSV"
     gene            5855..6625
                     /gene="ceg19"
                     /locus_tag="ACF2SP_002565"
     CDS             5855..6625
                     /gene="ceg19"
                     /locus_tag="ACF2SP_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg19"
                     /protein_id="HFD2360338.1"
                     /translation="MFKKIITKLGIVMSKLRALEKEANELSLILDQALLSQSIEVKRR
                     ALSQFENSELIQNKDNLRVFIDPEHPIVSLIILATKTLPEKIENLQKKVRLLEIQLTA
                     ETEETLDESNSDSESVDYDNGIDEETVVPVTVTHQEHVSEPDARSVAIARLREALKPY
                     IDSTQERTYEHYYGMAGTLFSFFGAAGYSKTDKLNAITKLFQNLEGDGAEPLNDTDRD
                     ILTTGVLGTTLKPLLEDEHIGNELKDLLSIKTDNVNTI"
BASE COUNT         2028 a         1309 c         1259 g         2071 t
ORIGIN      
        1 aataaagaac acaagttttt gcttttaatt cattaaccta ctattttgtg gaatggaaag
       61 taaaccagcg aatcaaccat cccaattggc atgtggtttg gttatttcat ttaagccaaa
      121 aagctaattg taaaaaaacc gacaattttc atatagctta ttgttaaata agaaaaaatg
      181 atgatagatt gatttttttc caatcataaa tgaggagggg ttgatgcgat attctttatt
      241 actgccaggt attttaactg tgattgcgac aagcgtcagt gccaatacct ctttaaaacc
      301 ctcaattgcc caacctgcaa cctgtgtaaa ctctcttttt acagcaacgg gaaatcagca
      361 ctggaaatca attattttaa agttaaccaa taattgcaat caggcagtcg attttcaaaa
      421 ttcaaccgtc tcctttcaaa cagcggaggc gttaaacacc tccttttggg gcgatttttc
      481 tcctttatct tacccggata atgctttaaa tatttcttct caacctcaat ctggcggaaa
      541 ctatctagcc actttcaacc tgcattttcc cagctacccg ggtgctaata gtaagttacc
      601 tgtcggaagt tctatttcaa ttaaatatgg cgcaaccact gatagtcata ttgagggaac
      661 agctaatgtt tatttaagca caacggttga agcaggaagc atccaattaa ttaatgcggc
      721 cacaaaacca acaaacgtgt cacaagggta tgcgctagtg catgtcacca tgaatggtca
      781 atcaatcagt gatgtgcaat taccctggca aacctcaaag acgctttccg gtcttgctgc
      841 gggcaattat gccatttctg ctgaaacggt cactgacagt aatggcaatc tctatctcgg
      901 gcaagccaat cccagcatgg tgagtgtgat tgcaaacgaa acaacgagct cgactattaa
      961 ttatgctcgg gtacaagaga caggcaagat caagatccac gtgcagaatc ttcctggcga
     1021 attaaacaac tacaatgaaa atccaactgt tctgcttacc cagagtcaat caggaaattc
     1081 acgctcacag cctgccgttt gggggaacac aacaactgtt gctgagttga aagaggggag
     1141 tagttatcaa ttttcaactc ctgcaattca atataacgat tacaattgct ggcccacttt
     1201 cacgccgttt tccttggtgg caagtgctac aaatgttcca actaccaatt tgacttatca
     1261 atgtatccag gttgttaaag atactgtgac acttagtgta agtggtgctc cagcctccct
     1321 ggcctcattg aaaattattt taactcccaa cgatggttca caaacagtag aacagacagt
     1381 agatttggcc aatggtgttg gttcatctgc catttcttta actgacgggg ttatttatac
     1441 actttcaagt gaagctgttt ccggctatac agtacaattt tcgccccaac ctttaacagc
     1501 aacagcaaat gcaacagtaa acattacttt atctcaggta acctctgcaa aagggcgtat
     1561 tattggttac ataccgggat ggaaaactcc acccaccgct caggagttgg ctaacgcggg
     1621 ttatactcac gtcatgattg ctttcggcgt atttagcacc aatactccgg gtgttattgt
     1681 tcctgctttt gaaacagtaa ccaaagaata tattcagtct cttcatcaag ccgggattaa
     1741 agttattctt tctttaggtg gtgcgttaac cagcatcccc aatacaacgg tagactttca
     1801 ccaggttcta gcggcttctt cttcaccaga tgtatttaaa caaacattta tcaactcttt
     1861 aaaggggtta atttctcaat atggttttga tgggtttgat atggatattg agcatggtat
     1921 taacgctggc ggttcttttt ctcaaccaca aggtgacatt gctgtgttag caagcattat
     1981 taatacgatg tacagtcaaa atccttctct gttaattact ctgacacctc aagtcgctaa
     2041 tattgctgca acaagcggtt tcgaccaaac ctgggggaat tatgcctctt taactatgca
     2101 aacccatcag tctttagcgt gggtaggtat ccagctttac aatacaggat gtgctttcgg
     2161 aattgatcaa gtatgctatg gtcctacacc aactgatatc cctgattttt cagtagctat
     2221 ggctaccgat ttattggaga attggccagc aacggtcaat ggacgtccta caggatttca
     2281 accttatatt agctatttaa gaccttccca aattgtcatt ggttatcctt ctccaaatgc
     2341 tggtggtggc agtgatggtt caccggttac tccgacaacc acaatcaaac gggctattca
     2401 gtgccttaag acagcaattg ccagtaatac cagttgtggt gcttatgttc cgccaagagc
     2461 ttatgggaat atcggtggtg tatttaactg ggaagtaact tatgataaaa acaatcaatt
     2521 caaatttgca aaagaattga aaaattgtgt tattaatggc gtttgtgagt aatcaatttg
     2581 acgggtcagg ctctttgagc ctgacactta aatcttgaaa tattgtttat catgtattat
     2641 ctgtttgatc caatatattt taattttgta aatcatccgc tcaatgagag agcttagcgc
     2701 agattttttc catatcgcga tcacgcaggt gtctatcttg aggaaatttg taattggcaa
     2761 ccaaactttc tttagaaatt tcaaatactt ttccatcagc taaattagta tttaaagttt
     2821 ctccaccaaa tagggtaatg cgttttccat caaaacgaga attgaaatat tttttccaaa
     2881 acacatccga gcctggttga atcatcatat gaatgccttt gtccatttta agagcatatt
     2941 taatggctct tgaggcaagt tcactcccca atccatgtcc tctggcatta ttgtcaacat
     3001 aaagctggtc tatttctagc aaatttctgc tttctaacct ggctgatgag gcaagttgaa
     3061 catagccatg tatctctcct gttgacttgt gttttgcaga tatctgtata ttgccgtttt
     3121 gcatagttga gatttcaaat ttgtaaatag ccatattttg cccccataaa agcccagata
     3181 attttcactt agtataacat ataatctatt ttgcggactt ccattagatg agcgcagtta
     3241 agcagttatt cagaattgtc aatcgcactg agagtttgag atgctatgtc ttcgatgata
     3301 tcactgtcaa aatagttggt taaaataaca acagtcaatt gccggtctgg aaaatagtag
     3361 tactggcctt ggtatcctga gcttgcccca ttatgggacc aggctatctc cccattaatt
     3421 tcttcacgtc ctattccaaa accgtaatca tctggaaagg taagcatcat tgttaaatag
     3481 tttggagata aaagtatttt atcttttaac aacgcttgga caaaaatatt gatatcttca
     3541 gcagtactaa ttattccacc atcagccaag ccaaaaccat catcatagtc tgttatgtca
     3601 accactttgc catcttccaa ggtataacca tgtgttgata aataatgatg gtcgtcatca
     3661 aattcttgct tttctatata tgtatttttt aaacgaagag gtttcaggat gtatttctta
     3721 attgattcgg cataggattc tccggttaag ttttcgataa ttaattgcag caacaaataa
     3781 ttagtattgc tgtactcata agctgtccct ggcttaaagt caggtttttg atcgtagact
     3841 agtttaatgc attcttctgg tgtccattcc ttgtctgcca tagtatcaat gagatcactg
     3901 taagaatcat aatccgtata attaggaatg ccgctacgca tttgcagtaa ttgttgcact
     3961 gttacctcat ctccattcgg tatgcgttca atatctatac tatctggcaa taagtctgca
     4021 atatgatcat caagatttaa ctgtttatct gctaccagtt ttaagacagt tacagccaaa
     4081 aacgttttac tcatgcttgc aagacgaaaa ctgtcggtaa tattcattga aatatcattt
     4141 tccttatctg ccaaccctgc cgcgacggaa attgtgccca aatcaggact tgatattagt
     4201 aatactgctc caggggttaa tgagtctgcg agattgtctt ttaatatggt atgtaattgc
     4261 ttggttaagg aagggctcgc aaaggcaagt gggcaattat taaagacaat aaaaattaaa
     4321 aacgtataaa tcaatttcca cttgcataca taacggctca ttgcaactcc aatgtggcaa
     4381 gggttttctc acccaccaaa tgagctatat cttaattcta gtcgatttca ggataatttt
     4441 tttacttctt ttataaaata gattgctcct ccaggttcaa aatgaatcaa ggaatacaaa
     4501 agccgatgtc ttgattttta tttgtgtctt cgtcatagtg cgaaaccgag attaaccaac
     4561 ggtggcaaga tacaacaaga attttttacg aagcttcatg tttactttaa ctcaaggagt
     4621 atgaatacat ggtcaacaag atttgcctga gtttcgcact attaattagc gtaccctcca
     4681 ttttattagc agaagacaaa cttatttttg ctgtagacat tattcgtcat ggcgacagaa
     4741 cgccgattgt tgctttgcct actgtaaatt accaatggca agaggggctt ggccagctta
     4801 cggcagaagg tatgcaacaa gaatacaaaa tgggtgtggt atttcgcaaa aaatatattg
     4861 aagagtcgca tttgttacct gaacactatg aatacggaac catctatgtc cgctctacgg
     4921 attatgcacg tactctgatg agcgcccagt cattgttaat ggggttatat ccacctggaa
     4981 ctggaccagc aattcctgca ggaacttccg ctttacctca tgctttccag cctattcccg
     5041 tatttagcgc accgtctaaa tacgacgagg ttatcattca gcaggtagat cgtaaggaac
     5101 gggaaaagct aatggagcaa tatgtttttt ctaccaggga gtggcaacaa aaaaataatg
     5161 aattgaaaga caaataccca ttatggagcc gcctgactgg tattaatatt gataatctgg
     5221 aggatctgga aacagttggt cataccttat atattcatca aatccataat gctcctatgc
     5281 cggaaggatt agcttctaac gatattgaaa caattatcaa ttccgctgag tgggctttta
     5341 tggcacaaga aaaacctcaa caaatagcga atgcttatag ttcaaagctg atgactaaca
     5401 tagctgatta tctgaattcc ggaagcatga aaaaatctaa actgaaatat gttttgctat
     5461 ctgctcatga tactacaata gccagtgtat taagtttttt aggtgctcct ttggaaaaat
     5521 ccccacctta tgcctcaaat gttaattttt ctctctatga taatggagca aattattaca
     5581 aagtaaaaat tacctataat ggtaatccgg tacttattcc tgcttgcggt ggttcagtat
     5641 gtgagttaca acaacttata aatttggtgc atgattctaa aaattcagtg tgatcactca
     5701 tcctggaggt atttggctga ggtgagtaag tctaagcacc tcaaactatc tgttgttgca
     5761 agagagcaac agcagctggt ttgaggcatt cgggaaaata gcccattttt cataagttaa
     5821 gattatctta atgtttttgt tttataatta atcactgttt aaaaaaatta tcactaaatt
     5881 gggaatagtt atgtctaaac taagagcatt ggaaaaagaa gcgaatgaat tgtctctcat
     5941 tcttgatcag gccctcttga gtcaatcaat agaggttaaa agaagagcat tatctcaatt
     6001 tgaaaattca gaattaatac aaaacaaaga taatttgcgg gtttttattg accctgagca
     6061 tcccattgtg agcttaatca tacttgctac aaagacttta cctgaaaaaa tagaaaatct
     6121 gcaaaaaaag gtacgtttgc tcgagatcca attgactgct gaaactgagg aaacccttga
     6181 tgaatccaat tcggattctg aaagcgttga ttacgataac ggtattgatg aagagactgt
     6241 tgttcctgtc accgttactc atcaagagca tgtcagtgag cctgacgcca ggtctgtggc
     6301 cattgcacga ttaagagaag cgttgaaacc ttatatcgat tctactcaag aaaggacata
     6361 tgaacactat tatggaatgg cagggacact gttcagtttt tttggagcgg caggttacag
     6421 taaaactgat aaactaaacg ctatcacgaa attgttccaa aatcttgagg gggatggcgc
     6481 tgagccactc aatgatacgg atagagatat actgactaca ggagttcttg gcactactct
     6541 gaagccgttg ttagaagatg agcatattgg taatgaattg aaagacttgc tatcgataaa
     6601 aaccgataat gtgaatacaa tttaaaaact catatttgaa caggagtttt cctgttcatc
     6661 taaaaca
//
LOCUS       DAXLDT010000158         6637 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXLDT010000158 DAXLDT010000000
VERSION     DAXLDT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.158"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(98..913)
                     /locus_tag="ACF2SP_002566"
     CDS             complement(98..913)
                     /locus_tag="ACF2SP_002566"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFD2360339.1"
                     /translation="MFFHPKMLFMLFILLVCTEVLAHNEDYIFDPNDPHHSLYIPDAT
                     MRSPAILLLHASTGIEKVNYDWATRLKEHGYVVYMIDSFKPRGWEDRRSVGWERATQA
                     QLDDIVPAYEYLKQLPSVDPDRIGLLGFSMGGFDVLKIMALSQQDPKPYQKLSFKAAA
                     SFYGVCHRLEEGTKLRAITKLFIGEHDDRATTQDCIQLVNQSTGSNQNVSIQIYENAL
                     HGFDNFEFPVSKEVIDEKGEHYHIGFNEAARAQALKDLPEFFDGYLKINPENK"
     gene            complement(932..1651)
                     /locus_tag="ACF2SP_002567"
     CDS             complement(932..1651)
                     /locus_tag="ACF2SP_002567"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HFD2360340.1"
                     /translation="MKLKIEHSYVELCKRLSQLTTPLICDALPSVRLMDSDIHSFNTS
                     PQTRIVGRAYTVNSAQDSLSTMQALDDLPDFMLALGCYDDRVPILLVIASCGTKLAVA
                     GGMCADVAKTKGFGGIITDGNCRDVSEVRTTNIPFFAKGQCAKSGSKNKIGSIRKPIN
                     CGSVEVNPGELIFADEDGIIVMNKEEAVNAINKAEEIKEKEAAALTRIHEGARFDEIC
                     NLKEHMEKLENNEASSLKLTV"
     gene            complement(1700..3295)
                     /locus_tag="ACF2SP_002568"
     CDS             complement(1700..3295)
                     /locus_tag="ACF2SP_002568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360341.1"
                     /translation="MMFKKVSSLFIALSIFNGLTYSSSINTTLAVVEENADRISFYNP
                     DTGARKGSLKLAFLPHEIAVTKDGKTAFVSNFGIRDYDSGSGIPGVSISVIDLLNQVE
                     KYRLFTFESLENKDYADIDSAPHGVKLRPPFEKQLFVNVEKGGKILVFDVDTKTIVKK
                     IAVSPNTHNLFFSNDGKTLWLMAGKDGVIRIDPDSGIITGNLTLPSAVRGLKYTPDHR
                     SLMVSAVNQIVFIDPDTLAIKKQFTNLNLGPILYSDITPDQNYILAPAPFDHQVAVID
                     VHSGQVIKRLVTGLNPINVLIDPEGQYAYVSNATDKHLSKIDLHNFELISIPTHAGPN
                     GLAFIPEFTQRTHKKLRMGVALPLTGKDGSKGREMLRGYEYWKSTVIKSGGLLIDNQV
                     YDPDIVYLDTESQKDKLKSLTQELLKQYQIQILLSTYGIDNYNLEKEIAEANQIILTP
                     APGEEILWKPDNIARGYDYFVTTNLYEKGYVTQYNFKPSSWSAMASVIGLKFQNASQT
                     ANTLDYQQITTLLNEGDFHLFYP"
     gene            3399..3848
                     /locus_tag="ACF2SP_002569"
     CDS             3399..3848
                     /locus_tag="ACF2SP_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD2360342.1"
                     /translation="MTFNLNEHTGVLIKKAARLFERVSNLNLEELGVTYSQTIFLVRL
                     WERDGQTQMELTKSAGLKQPTVVGILDKMERDQLITRVRSEQDKRCYHFFLTEKAKHA
                     CRELEKQGILMQNLSAGNLPNKEVSKLNENLLSIIENLERFIEKIQG"
     gene            complement(3826..5400)
                     /locus_tag="ACF2SP_002570"
     CDS             complement(3826..5400)
                     /locus_tag="ACF2SP_002570"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFD2360343.1"
                     /translation="MPKVIAIAGMGLCGNAIFCRLSHLLATMKYSDDDITILIFEQNP
                     QYLATGFAYSIDSPEFWTLNNPADGFKFTSTTDSLTEWIDANPELLQDHYPHRDKQYI
                     PRAVVGHYLRAQYEKHKTLVKSLGITVKEIFLPISDICPLKEGNYQIACMDQNYIADR
                     LFITTGHLTNPFFHTVENQPGFLRFENTTGNPDAWDLADDVYIIGGQAAFVDAALWLT
                     MKKQYSGKIHSVTRNPNILTTKGNTDSCDTTKQQELSNSLQKRFPDSLTFSEAKTLFW
                     NAYQNDAKNPITPTHSPGALETLRYQMNKFDHLPENNPILGNIDELRAFIKTFYFSGC
                     YTHMWQSLNVEGKEQFLKYFYSLLMAYLTGITPVNARIMLHLYEKNRIIEHHSLASVS
                     YDEVNHRFQLLFTSGEVLHAATLIDATGYRYKPDPNSVKPMLLDKLAQTGLISTKPYG
                     GIHLTDHYQVIDTYGKVHENLVCIGPVANYNHPVPTPHSSFMVYRDVDLVMDQFASTF
                     QVTEHGAIKSTPEFFQ"
     gene            5697..5978
                     /locus_tag="ACF2SP_002571"
     CDS             5697..5978
                     /locus_tag="ACF2SP_002571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360344.1"
                     /translation="MQDAITIENNWRSRGFSFELWEDPPGQIWKDFKHETDELFMLLE
                     GEVILTVGNKTLKPAIGGIDFRRSRSYGSNQFHHRKSLVLWLQNKKVRE"
     gene            5975..6130
                     /locus_tag="ACF2SP_002572"
     CDS             5975..6130
                     /locus_tag="ACF2SP_002572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFD2360345.1"
                     /translation="MTQKKISLENDAVLQFIVDELISLHHCHTIILYGSRARGDFTVT
                     SDYDCRD"
     gene            6114..6515
                     /locus_tag="ACF2SP_002573"
     CDS             6114..6515
                     /locus_tag="ACF2SP_002573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360346.1"
                     /translation="MIAGIREKGDKQRIARFDEAHQVYHDIFVYPENAFDSISDEHLC
                     MSDGIVVLEKAHFGTELLKKLSAFLILPESLPPDEITARRVWYQKMLARAYTRDLEGK
                     YRHIWSIFTILEDYFVFKELRYQGPKKHSSI"
BASE COUNT         2072 a         1168 c         1263 g         2134 t
ORIGIN      
        1 ttgctcatca ataaatgcat agctattgtt aataattgat agaaacaaac ttatagcaaa
       61 gatttttgat ttattaataa tcataaaaca ccattggtta tttattctca gggtttatct
      121 tgagatagcc atcaaaaaat tcgggtaaat ctttcaatgc ttgtgctctt gctgcttcat
      181 taaaaccaat atgataatgt tctccttttt cgtcaataac ttcttttgaa actggaaact
      241 caaagttatc aaatccatgc aatgcatttt cataaatttg gatggaaaca ttttgattgc
      301 taccagtact ttggttgact aactgaatgc aatcttgggt tgttgcacga tcatcatgtt
      361 ctccaataaa tagtttggtg attgccctta gttttgttcc ttcttcaagt cgatgacata
      421 cgccataaaa agaagccgct gctttaaaag agagcttttg gtatggtttc ggatcttgtt
      481 gtgataacgc catgattttc aatacatcaa agcctcccat agagaatccc aaaagtccta
      541 ttcggtcagg atcaacagaa ggaagctgct taaggtattc ataggcagga acgatatcat
      601 caagttgtgc ttgtgttgct ctttcccagc ctactgagcg acgatcttcc caacctctgg
      661 gtttaaaact gtctatcata tagactacgt atccatgctc ttttaatcgg gtggcccaat
      721 cataattcac tttttcgata ccggtactcg cgtgcaataa taaaatagca ggggatctca
      781 tcgttgcatc aggaatataa agactgtgat gtggatcatt gggatcaaag atataatctt
      841 cattatgtgc tagtacttca gtacacacta ataaaataaa taacataaaa agcattttgg
      901 gatggaaaaa cataatttta attccttagt tttaaacagt tagctttaga ctggatgcct
      961 cattattttc tagcttttcc atgtgctctt ttagattgca aatttcatca aaacgagcac
     1021 cttcatgaat tcgagtgagg gcagccgctt ctttttcctt aatctcttcc gctttattaa
     1081 tagcattaac tgcttcttct ttattcatga caataatgcc gtcttcgtca gcaaatatca
     1141 gctcaccagg atttacctca acagaaccac aattgattgg ttttcttata ctgccaattt
     1201 tatttttact tcctgatttg gcgcattgtc ccttagcaaa aaaagggata tttgttgttc
     1261 tgacttcaga aacatctcga caatttccat cagtaataat gccaccaaaa cccttcgttt
     1321 ttgctacatc tgcacacatg ccacctgcta cagcaagctt ggtgccgcag gaagcgataa
     1381 ccaataaaat gggaactcta tcatcataac aaccaagggc aagcataaaa tcaggcaagt
     1441 catccaaagc ctgcatggta gataaagaat cttgagccga gttaacagta taagctcttc
     1501 ctacaattct tgtttgtggc gatgtgttaa aagaatgaat atctgaatcc atgagacgca
     1561 cactgggtaa agcatcacag attaagggcg ttgtcagttg agataatcgc ttacatagtt
     1621 caacatagct atgttcgatc tttagtttca taataatcct taatatatcg attcagttct
     1681 atccaatttg tatgaggttt tagggataaa ataggtgaaa atctccctca tttaataaag
     1741 ttgttatttg ttgataatct aaagtatttg ctgtttgaga agcattttgg aatttcaatc
     1801 cgatgacgga tgccattgct gaccaggagg agggtttaaa attgtactgt gtaacatagc
     1861 ctttctcata aaggtttgtt gttacaaaat aatcataacc tcgagcaata ttatcaggtt
     1921 tccaaagtat ttcttctcca ggtgctgggg taaggataat ttggtttgct tctgctattt
     1981 ctttttcaag attgtaatta tcaataccat aggtagataa taatatttgt atttgatatt
     2041 gcttgagtaa ttcctgagtc aagcttttga gtttatcttt ctgggattcg gtgtctagat
     2101 aaacaatatc gggatcataa acttgattgt caattaaaag tccaccgctt ttgataacag
     2161 tacttttcca atattcatat cctcttagca tttcacgacc tttagaacca tccttccctg
     2221 tcaatggtaa agcaactccc atgcgcagtt ttttgtgtgt tctttgggta aactcaggga
     2281 tgaatgctaa accatttggt ccagcatggg ttggaataga gatgagttca aaattatgca
     2341 aatctatttt agataaatgc ttgtctgttg cattagatac ataagcatat tgaccctctg
     2401 ggtcaattaa aacgttaatc ggatttaatc cggtgactaa tcgttttatc acttggcctg
     2461 aatgaacatc aatcactgca acttggtgat caaagggcgc cggtgctaat atatagtttt
     2521 gatcaggagt aatatctgag taaagaatcg gtcctagatt caaattagtg aattgttttt
     2581 taatggcgag ggtatcggga tcaataaaga cgatctgatt gacagctgat accatcaagg
     2641 aacgatgatc tggcgtatat tttaaacccc gtacagctga gggcaatgtg aggtttccag
     2701 taattattcc tgaatcagga tcgattcgaa tgacgccatc tttacccgcc attaaccaca
     2761 aggtttttcc atcattagaa aaaaacagat tatgtgtatt aggactgacc gcaatttttt
     2821 tgacgatggt tttggtatca acatcaaaga cgagaatctt accgcctttt tccacattca
     2881 caaaaagttg tttttcaaaa ggaggtcgta acttaacacc atgaggcgca ctatcgatgt
     2941 ctgcatagtc tttgttctct agtgattcaa aagtaaatag ccgatatttc tcgacttgat
     3001 ttaataaatc gataacagaa atggacacac caggtatacc agagccacta tcgtagtctc
     3061 taatgccgaa attgctgaca aaagctgttt tcccatcttt tgtaacagca atctcatgag
     3121 ggaggaaggc aagtttcagg ctaccttttc ttgcacctgt atcgggatta tagaaactga
     3181 tacgatcggc attttcttca acaacagcta acgtggtgtt gatagaagag gaataagtga
     3241 ggccattgaa aatcgataga gcaataaata ggctgctaac ttttttgaac atcattaaat
     3301 ccctttataa acacaaaaag tgccgccatg atagtaaggg aatacgatat tgtcaatata
     3361 tataggtgcc tatataatag cgcaaaaagg aactatccat gacatttaat ttgaatgaac
     3421 atactggtgt cttgattaaa aaggcagctc gcttgtttga acgtgtttca aatctcaacc
     3481 ttgaagaact tggtgtgacc tatagtcaga ctatttttct ggttcgcttg tgggagcggg
     3541 acggccaaac gcaaatggaa ctcacaaaaa gtgcgggact gaaacaacct accgtagtag
     3601 gtatacttga taagatggaa agagaccagc ttatcacacg tgttcgaagt gaacaggaca
     3661 aacgttgcta tcattttttt cttactgaaa aagcgaaaca tgcttgtcgc gaattagaaa
     3721 agcaggggat attgatgcag aatttgtctg cgggtaattt acccaataaa gaagttagca
     3781 agttaaatga gaatctatta tcaattatag aaaacctaga gcgttttatt gaaaaaattc
     3841 aggggtagac ttgatggccc catgttcagt cacctgaaat gtagaagcaa attgatccat
     3901 caccaaatct acatcacgat aaaccataaa tgaagaatgt ggtgtaggaa caggatgatt
     3961 gtaatttgct acaggcccta tacaaactag attttcatgt acctttccgt atgtatcgat
     4021 cacttgataa tgatccgtta aatggatgcc accataaggc ttagtgctta ttaatccggt
     4081 ttgtgccaac ttgtctaata acatgggctt gacactattg ggatctggtt tgtaacgata
     4141 tccagttgca tcaataagag ttgcagcatg caagacttcg cctgatgtaa ataataattg
     4201 gaatctgtga tttacttcat catagcttac agaagccaag ctatgatgct caataattct
     4261 atttttctca tagagatgga gcatgattct agcattcact ggtgtgatac cagtcaaata
     4321 agccattaaa agagaataga agtattttag aaattgttct ttgccttcaa cattcaagct
     4381 ttgccacatg tgagtataac aaccactaaa ataaaaagtt ttaataaatg ctcgtagctc
     4441 atcaatatta cccagtatgg gattgttttc tggcagatga tcaaatttat tcatttgata
     4501 acgtaaagtc tcaagagcac caggggaatg ggtaggagta ataggatttt tagcatcatt
     4561 ttgataagca ttccaaaata atgtttttgc ttcggaaaag gttaaactgt caggaaagcg
     4621 tttttgtaga ctatttgata attcctgttg tttcgtagtg tcacaactat ctgtattgcc
     4681 tttggttgtt aatatattag gatttcgcgt gacactgtgt atttttcctg agtactgctt
     4741 tttcatagtt aaccaaagtg cggcatcaac aaatgctgcc tgaccaccaa tgatataaac
     4801 atcatctgct aaatcccatg catctggatt tcctgttgtg ttttcaaaac ggagaaaacc
     4861 tggttgattc tcgacagtat gaaaaaaagg atttgttaaa tgtccagttg ttataaaaag
     4921 tctatcagcg atataatttt gatccatgca tgctatctga taattccctt cttttagtgg
     4981 acaaatatct gagattggca aaaaaatttc tttgactgtt atccctagtg atttaaccag
     5041 tgttttatgt ttttcatatt gagcgcgtaa ataatgaccc actacagcac gaggaatata
     5101 ttgtttgtct ctatgtggat aatgatcttg gagcaactct ggattagcat ctatccattc
     5161 tgttaggcta tctgtagtag aagtaaattt gaaaccatca gctggattat ttaaagtcca
     5221 gaattctggg ctatcaatag aataagcaaa tcctgtagca aggtattgtg gattttgttc
     5281 aaagattaag atagtaatgt catcgtcaga atatttcatt gttgcaagca agtgacttaa
     5341 acggcaaaaa atagcatttc cgcagagtcc cattcccgca atagcaatta cttttggcat
     5401 tatatcaaga caattgttgg atcagaggaa aattctaggg tttaacctcg atactgtcaa
     5461 tttgtagatt tcttatcttt aaatgacaac agattgatcg atccacatcc ttaatttaga
     5521 gctaaatcgt ccctgacggc aaagagtttt gacggccttt gtagtgatgc ttttaattaa
     5581 gcgatattcc cccagtactg catgtataga ccaacattgg ttaataattg tatagtctat
     5641 tattcttcag tttcactggt gtaaaattga aagtgtcttt taaataggag tccttaatgc
     5701 aggatgcaat aacgattgag aacaattggc gctctcgtgg ctttagtttt gagctttggg
     5761 aagatccccc aggtcaaatt tggaaggatt ttaagcatga aacggatgag ttattcatgt
     5821 tgcttgaagg tgaagtcatt ttaaccgtgg gcaataaaac gttaaaacct gctattggag
     5881 gtattgattt caggaggagt agatcatacg gttcaaacca gttccaccac aggaagtcct
     5941 tggtattatg gttacaaaat aagaaggtaa gggaatgaca caaaagaaaa tatcactaga
     6001 aaatgatgct gttctgcaat tcattgttga tgaattaata tctttacatc attgccatac
     6061 aattatttta tatggctcgc gcgcacgagg agacttcaca gtaaccagtg attatgattg
     6121 cagggattag ggaaaaagga gataagcaac gaattgcacg ctttgatgaa gcacatcaag
     6181 tttatcatga tatttttgtt tatcccgaga atgcttttga ttcaatttct gatgaacatt
     6241 tatgtatgtc agatggcatc gttgttcttg aaaaagctca ttttggcaca gagttattaa
     6301 aaaaactctc tgctttttta atattgcctg agtccctccc tcctgatgaa attacagcac
     6361 gtagagtgtg gtatcaaaaa atgctggcaa gggcgtatac tagagattta gaaggaaaat
     6421 atcgccatat ttggtcaatc ttcacaattc tagaggatta ttttgtattc aaagaattgc
     6481 gttatcaagg tccaaaaaag cattcaagta tttagaaaca cacgatccag agactttatc
     6541 attatttgat gaggccataa cgaatatcga taatttggat gccttaaata ggctaattac
     6601 aagagttata aagatagaca aaacgtagga taattat
//
LOCUS       DAXLDT010000159         6591 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXLDT010000159 DAXLDT010000000
VERSION     DAXLDT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.159"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            90..620
                     /locus_tag="ACF2SP_002574"
     CDS             90..620
                     /locus_tag="ACF2SP_002574"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360347.1"
                     /translation="MITIRLAEMNEIQFLNQLISCSARELSQEDYTKEEIEGAIQYVF
                     GVDLELILDKTYFVIEKDGQIAGCGGWSRRKTLFGGSQYKGREQGVYLDPQQDFAKIR
                     AFFIHPKFARQGLGSSLLKHCEHEAFLQKFTRFEMMATLPGVKLYSAFGYHPTSNEVI
                     TLPNNVPLRFVRMTKG"
     gene            872..3445
                     /gene="lgt3"
                     /locus_tag="ACF2SP_002575"
                     /gene_synonym="legC5"
     CDS             872..3445
                     /gene="lgt3"
                     /locus_tag="ACF2SP_002575"
                     /gene_synonym="legC5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     Lgt3/LegC5"
                     /protein_id="HFD2360348.1"
                     /translation="MKEQQKAIFIENIKSTCAVDEQMAKELADSLLEIHQLNTGVNEQ
                     TLVNEVFLTKFALYLKIGKEVALDWVRKNKDLDTPFPSDTVKSQPVQDAYKPAENKVQ
                     VYGPMTIGKSPVKTTLNQLTGNLLVDVVEAGKKEKAQKLRAYIFDQLARRLEASLSQE
                     QINDLYNRIRGTGNYTKSESFSEEQLKILKEKIVPELKRELSDLGNGNVNILGLDVSR
                     EDKYAFDTTNIFSVWFSNNPAVYMPQHVKTQVEKTAKLNQPGKTRIVFSSLCLNETAQ
                     IDFQQWAKENNIELVDIDSIDLKSVSETDAQLLNLAKDELEAMRKGKGGNPAAASDLV
                     RWVDVIIGESSTYIDIDLPMNDKKVTVEVHSGFPVLLNMGSALTKDGQQPAMENPAFN
                     TDMIAYSKDKEARRQIIEGVAKKIIARYENCAKYIEESKNEELVRLKNTPGYQFLVKK
                     TDGKFDLCTLRAAVSEAHQDALSFATFFGAEYFAKTFATQELVPVIKEAIQHQNQDLL
                     TSVIENHIEKQHLNDYPKTPDGIKKLLKSFQGIVYKPLVMEFSGPSAVSSSWVEAISG
                     RSIPRNFEYLAEPMSQPLRVLQHHACVSGKANFSSDNIPKWCELQSDVEKRQLQREDG
                     LSWLPDAQEKLKREGQQAKLEEEQRIKLEEEQRIKLEEEQRIKLEEEQRIKLEEEQRI
                     KLEEEQRIKLEEEQRIKLEEEQRIKLEEEHKSKKYFAQSDAIDLILNNFENEINSIGA
                     YYAPAKQRAIELLDTLRKYKEEAFNDPSREKLISFAQNTKRAIQEATPILQKDLGWGD
                     YLANLAKQLVNAVTFAVAYAVTFGTTGHQGFFALKSSLAVSQSQNLDEELNSKLSQNN
                     C"
     gene            3655..4680
                     /locus_tag="ACF2SP_002576"
     CDS             3655..4680
                     /locus_tag="ACF2SP_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360349.1"
                     /translation="MRRSKAMQIPKKRTQILTISESSKEDLDVVSEYSDELDVSPNKE
                     TSKTNSKKLESTARKEQIKKLEGELYSHIQDAIELEEESFAEFSINTGRPSNYLFRFV
                     SEKEYLAILENLNQGKRIFGNKKKVFEEEDEKISTKAKGETFFAFDLKYAQKYFNKGT
                     RRDGVMLAIELLEEADTKLLSNSEIVRRIGDDPGMAGGRWAKCKKVKPGERGIVVVKC
                     EGVGSCKYPYTLGFREGEEGVSPFDVLFELTRSVKVLNKNGSCKTIYHYSVPTPKEKE
                     LINIVEDAENGIDEIHSDEFLSVVSSSPSSRFGFFTNKELSGISGLVNKAPEHIIPPS
                     DNTFSHH"
     gene            4846..6171
                     /locus_tag="ACF2SP_002577"
     CDS             4846..6171
                     /locus_tag="ACF2SP_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HFD2360350.1"
                     /translation="MSALYYNLNNHYVSHVIHSPEKRANRDNNPEKIMKKPLDTTEQN
                     RIAALYELKILDTPADERFDRIIRLTSGYLSVPIAFISFIDSKHQWFKSSCGIGLNKI
                     SRNDSFCHYTIQQSKPMIIPDALLDERFCNNPYVTNSPNIRFYAGFPLSTVDGFNVGT
                     LCAIDKKPRNLDETEIQILKDLATLAEDQVNLLDISLLERAVREKNISLTKAKKELEM
                     RNNFIRKVFGSFMSDEIVAALLESKSELKLGGEERKITILFSDLRNFTTLSEHFPADK
                     IVAALNNHYGKMVDVIEKYNGTVDSFIGDAIMVVFGAPHSGGDDAYRAIACALEMQAT
                     MQEVNAMNRNSSLPELAMGIGINTGYAIVGNIGSQKRMQYSAIGSPVNLASRIQDLTL
                     GGQILISEATYQEANHSLELNGHLRVKVKGIAAPITIYDVVGLDKKSDS"
     gene            6347..>6591
                     /locus_tag="ACF2SP_002578"
     CDS             6347..>6591
                     /locus_tag="ACF2SP_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360351.1"
                     /translation="MKHLAELEKLKEKCLEMSDKEFNYCFNKGDTSQRSINTIVARLM
                     NKIGLKEVFFKTDKDYVDWIEDVISARKKAALSPSTPV"
BASE COUNT         2270 a         1038 c         1341 g         1942 t
ORIGIN      
        1 aaaaagtaat ttttataaat aaaataagaa atttatttat atttgtttta tgttaactta
       61 cctccacaca gatttgcagg aggttaccaa tgattacaat cagattagct gaaatgaacg
      121 agattcaatt tttaaatcaa ttaatttctt gttcagcaag agagcttagc caggaagatt
      181 atacaaagga agaaattgaa ggagccattc aatatgtatt tggtgtagac ttagagttaa
      241 ttttagacaa aacttatttt gttatagaaa aggatgggca aattgctggt tgcggaggat
      301 ggagtcgaag aaaaacatta ttcggtggca gtcaatataa aggcagggag caaggagtat
      361 atctggatcc tcaacaagat tttgcaaaaa taagagcttt ttttattcat cccaaattcg
      421 caagacaagg attgggaagt tctctgttaa aacattgtga acatgaggct tttttacaaa
      481 aatttactcg gtttgagatg atggccactt taccgggagt taagctctat agcgcttttg
      541 gttatcaccc tacctcaaat gaagtgatta ctctccccaa taatgttccg ttaagatttg
      601 ttcggatgac gaaaggttag atggtaattc attttatact gtctggagtc attattgact
      661 catggttatt gaaacttaat ttatcgtcat aatgttagga acggttatct taatgaatac
      721 aatccgatta gattaataac gtaatttaag ttttacttaa gttatttatg atatattcat
      781 gaccactttg taaacattta gtttattttt ggtgttttct ccagttgaga caacaggaaa
      841 aatttagatt ataaccaggt gggataaata tatgaaagag caacaaaagg caatttttat
      901 tgagaatatc aaatcgacat gcgctgttga cgagcaaatg gctaaagaat tggctgattc
      961 attgcttgaa attcaccagt taaacactgg agttaatgaa caaacactgg ttaatgaggt
     1021 gtttttaact aaatttgctc tctatcttaa aattgggaaa gaggttgcct tagattgggt
     1081 tcgaaaaaat aaggacttgg atactccgtt tccttctgat actgttaaaa gtcagccagt
     1141 tcaagatgcg tacaagcctg ctgagaacaa ggttcaggta tatgggccta tgacaattgg
     1201 taaatctcct gttaaaacga ctttaaatca attgacaggc aacttgttgg tggacgtggt
     1261 tgaggccgga aaaaaagaga aagctcaaaa attacgagct tacatatttg accaattggc
     1321 aaggcgatta gaagcttcgc ttagccaaga gcagattaac gatttatata acagaataag
     1381 aggcactggt aactatacta aaagcgaatc attttccgaa gaacaactta aaattttaaa
     1441 agaaaaaatt gttccagaat taaagagaga gctaagtgac ctcggcaacg ggaatgttaa
     1501 tatcctgggg ttggacgtta gtcgtgaaga taaatacgca tttgatacaa ccaatatatt
     1561 ttctgtctgg tttagcaata atccagcggt ttacatgcca caacatgtta aaactcaagt
     1621 agaaaaaact gcaaaactta accagccagg caaaactcga attgttttta gctcattatg
     1681 cttgaatgag acagcgcaga ttgattttca gcaatgggca aaggaaaata acattgaatt
     1741 ggttgatata gattcaatag atttaaaatc agtcagtgaa actgatgctc aattgctaaa
     1801 tctagccaaa gatgagcttg aagcaatgag aaagggcaag ggaggaaatc ctgctgcagc
     1861 atccgatctg gtgcgttggg ttgatgtaat cattggagaa agcagtactt atattgatat
     1921 tgatttacca atgaatgata aaaaagttac agttgaggtt cattctggtt tccctgttct
     1981 actcaatatg gggtcagcgc tcacaaagga tggtcaacaa cctgctatgg aaaacccggc
     2041 tttcaatact gatatgattg cttatagcaa agataaagag gcacgtcgtc aaataatcga
     2101 gggggttgcc aaaaagatca ttgcgcgtta tgaaaactgc gcgaaatata ttgaggaaag
     2161 taaaaacgaa gagttagtac gtttaaaaaa tacgccaggt tatcaattcc ttgttaaaaa
     2221 aacagacggc aaatttgatt tatgtacttt aagggcggca gtttcagaag ctcaccagga
     2281 tgctttgtcg tttgcaactt tttttggcgc tgagtatttt gctaaaactt ttgctacaca
     2341 agaacttgta cctgtaatta aagaggcaat tcagcatcaa aatcaagatt tgcttacatc
     2401 tgttattgaa aatcatatag aaaaacaaca tctcaatgac tatcctaaga ctcccgatgg
     2461 cattaaaaaa ctattgaagt cgtttcaagg gattgtctat aaaccattgg ttatggagtt
     2521 ctcaggccca agtgctgtct catcatcttg ggtggaggct atatctggaa gatccatccc
     2581 gaggaatttt gaatatttag ctgaacctat gtctcaacca ttacgagttt tgcagcatca
     2641 tgcctgtgta tctggaaaag ctaatttttc ttctgataac attcctaaat ggtgtgaact
     2701 tcagtctgat gtagaaaaac gacaactaca aagggaagat ggactttcct ggcttcctga
     2761 tgctcaagag aagcttaaga gggaagggca gcaagccaaa cttgaagaag agcaacgaat
     2821 caagcttgaa gaagagcaac gaatcaagct tgaggaagag caacgaatca agcttgagga
     2881 agagcaacga atcaagcttg aggaagagca acgaatcaaa cttgaggaag agcaacgaat
     2941 caagcttgag gaagagcaac gaatcaagct tgaggaagag caacgaatca aacttgagga
     3001 agagcacaaa agcaaaaaat actttgctca gtccgatgca attgatctca ttttgaataa
     3061 ttttgaaaat gaaattaact ctattggtgc ttattatgct cctgcaaaac aaagagctat
     3121 agagttgctg gatactttga gaaaatataa agaggaagca tttaatgatc caagcagaga
     3181 aaaactaatc tcttttgcgc aaaatacaaa aagagcaatt caggaagcaa caccaatact
     3241 gcaaaaagat ttaggttggg gagattattt agccaattta gcgaaacaat tggttaatgc
     3301 agtgactttt gctgtggctt atgctgtaac ttttggaacc accgggcatc aaggcttttt
     3361 tgctttgaaa tcttctctgg cggttagtca atctcaaaat cttgatgagg aactcaatag
     3421 caaattaagt caaaataact gttagggaat aggccagatg tagaatgcta atgggcaaga
     3481 ctttattttt gctgcccaag cattcttctt agatctaatg tatttcgatt cggtaatttt
     3541 gatttagaaa tgaaagattg caatccattt cattttgctg ataatgtgca aaatagattg
     3601 tgtgagaaca atataagcac atattgtata tcgtaacgat gttaggagag ttagatgcgt
     3661 agaagtaaag caatgcaaat acctaaaaaa agaacacaaa ttttaactat ttcagaatca
     3721 agtaaagaag atttggatgt tgtatccgag tattctgacg aactggatgt ttctcccaac
     3781 aaagaaacaa gcaaaactaa cagtaaaaaa ctggagagta ctgcccgtaa agaacagatc
     3841 aagaaattgg aaggagagct ttacagtcat attcaagatg ctatcgaatt agaagaagaa
     3901 tcttttgcag aattcagcat taacactgga agacctagta attatttatt tcgctttgtc
     3961 agtgaaaaag aatatttggc aattcttgaa aatttaaatc aaggaaagag aatatttggt
     4021 aacaagaaaa aagtatttga agaagaggac gaaaagatat caactaaagc aaaaggggaa
     4081 actttttttg cttttgatct taaatatgct caaaaatatt tcaataaagg tacaaggcgt
     4141 gatggagtga tgcttgcgat agaattgctc gaggaagctg ataccaaact attatctaat
     4201 agtgaaattg ttagaaggat cggagatgat cctggaatgg ctggaggccg atgggcgaag
     4261 tgtaagaaag taaaacccgg cgaaaggggg atagtagtag tcaagtgcga aggtgttggc
     4321 tcatgtaaat atccatatac cttaggtttt agagaagggg aagagggtgt aagtcctttt
     4381 gatgtactat ttgagttaac gcgctctgta aaagtactga acaagaatgg cagttgtaaa
     4441 actatttatc attattctgt tcctacacca aaagaaaaag agcttataaa tattgtggaa
     4501 gatgctgaaa atggtataga cgagattcat agtgatgaat ttttgtcagt tgtttcaagt
     4561 tctccttcat cacgctttgg tttctttaca aacaaggagc tctcgggtat ttcaggattg
     4621 gtaaataagg caccggaaca tataatacca ccctctgata ataccttctc gcatcattaa
     4681 atcaggttaa atttttaagg ctttagacgg gcaattaaat actatttatt tgcccgtttc
     4741 tgcgtatccc ctccataaca agggttagcc acatacaagt aacccatcaa ctttatttcc
     4801 attgctcaat acaatttact tattcgttgg tttaaaccaa ttaatatgag cgcattgtac
     4861 tataatctta ataaccacta tgtctctcat gttattcatt cacctgagaa aagagccaat
     4921 cgtgataaca atcctgagaa aattatgaag aaacctttgg atactactga acagaatcgc
     4981 attgctgcat tatacgaatt aaaaattctg gatacaccag cagatgaaag atttgatcga
     5041 attatccgac tcacttcggg ttacttatca gttcctattg cctttatttc ttttattgat
     5101 tctaaacacc aatggtttaa atccagctgt ggcataggcc ttaacaaaat atcccggaat
     5161 gattcatttt gccactacac cattcagcaa agtaaaccga tgattattcc ggacgccttg
     5221 ttggatgagc gattttgtaa taatccctat gtgactaatt caccaaatat tcgtttttat
     5281 gcaggatttc cattatctac agttgatggt tttaatgtgg gaacactatg cgcaatagat
     5341 aaaaaaccac gtaatttaga cgagactgaa attcaaattt taaaagattt ggcaactttg
     5401 gcagaagatc aagtcaatct gcttgatatt tcgcttcttg agagagctgt acgagaaaaa
     5461 aacatttcgc tgacaaaagc taagaaagaa cttgaaatgc gtaacaattt tattcgtaaa
     5521 gtatttggta gctttatgtc tgacgaaatt gttgcagcac ttcttgaatc aaaatcagag
     5581 ttaaaattag ggggggagga gcgcaaaatt acaattttgt ttagcgactt aagaaatttt
     5641 actactctgt cggagcattt tcctgcggat aaaattgttg ctgcgcttaa caatcattac
     5701 ggaaaaatgg ttgatgtcat agaaaaatac aatggcactg tcgattcctt tattggtgat
     5761 gccattatgg tggtatttgg agcaccacat tctggagggg atgacgcgta cagagcgata
     5821 gcttgtgcat tagagatgca ggcaaccatg caagaggtga acgcaatgaa tcgcaacagc
     5881 agtttacccg aattggcaat gggcataggg attaatacag gttatgctat tgtaggtaac
     5941 attggttctc agaaacgtat gcaatatagt gcaattggtt ctccggttaa tctggcatca
     6001 cgcatccaag atctgacttt gggggggcaa attttaattt ctgaggcaac ttaccaggaa
     6061 gctaatcatt ctcttgagtt aaatgggcat ctcagagtaa aagttaaggg aattgctgct
     6121 cctatcacta tttatgatgt agtcggtctg gataaaaaat cagattcata gacgtgttaa
     6181 tatcactttt taaaatcaag agccaaatag aaatttccga ataaacttta tataaatcgt
     6241 ttaatattta tcatattatg taatttatat atgctataat tttatctatt agtcagattg
     6301 aattcaaatt atcaaaaaca atgcactgtc actaggatat aaaattatga agcatttagc
     6361 tgagttagaa aaacttaaag aaaaatgtct ggagatgagt gataaggagt ttaattattg
     6421 ttttaacaaa ggagatacct cacaacgcag cattaataca atagtagcgc gtcttatgaa
     6481 caaaataggt cttaaggagg tgtttttcaa gacagataaa gattatgttg actggataga
     6541 agacgtaatt tccgcaagaa aaaaagcggc tttatctccc agcactccgg t
//
LOCUS       DAXLDT010000160         6495 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXLDT010000160 DAXLDT010000000
VERSION     DAXLDT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.160"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            305..4423
                     /gene="cas9"
                     /locus_tag="ACF2SP_002579"
     CDS             305..4423
                     /gene="cas9"
                     /locus_tag="ACF2SP_002579"
                     /inference="COORDINATES: protein motif:HMM:TIGR03031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II-B CRISPR-associated RNA-guided
                     endonuclease Cas9/Csx12"
                     /protein_id="HFD2360352.1"
                     /translation="MESSQILSPIGIDLGGKFTGVCLSHLEAFAELPNHANTKYSVIL
                     IDHNNFQLSQAQRRATRHRVRNKKRNQFVKRVALQLFQHILSRDLNAKEETALCHYLN
                     NRGYTYVDTDLDEYIKDETTINLLKELLPSESEHNFIDWFLQKMQSSEFRKILVSKVE
                     EKKDDKELKNAVKNIKNFITGFEKNSVEGHRHRKVYFENIKSDITKDNQLDSIKKKIP
                     SVCLSNLLGHLSNLQWKNLHRYLAKNPKQFDEQTFGNEFLRMLKNFRHLKGSQESLAV
                     RNLIQQLEQSQDYISILEKTPPEITIPPYEARTNTGMEKDQSLLLNPEKLNNLYPNWR
                     NLIPGIIDAHPFLEKDLEHTKLRDRKRIISPSKQDEKRDSYILQRYLDLNKKIDKFKI
                     KKQLSFLGQGKQLPANLIETQKEMETHFNSSLVSVLIQIASAYNKEREDAAQGIWFDN
                     AFSLCELSNINPPRKQKILPLLVGAILSEDFINNKDKWAKFKIFWNTHKIGRTSLKSK
                     CKEIEEARKNSGNAFKIDYEEALNHPEHSNNKALIKIIQTIPDIIQAIQSHLGHNDSQ
                     ALIYHNPFSLSQLYTILETKRDGFHKNCVAVTCENYWRSQKTEIDPEISYASRLPADS
                     VRPFDGVLARMMQRLAYEIAMAKWEQIKHIPDNSSLLIPIYLEQNRFEFEESFKKIKG
                     SSSDKTLEQAIEKQNIQWEEKFQRIINASMNICPYKGASIGGQGEIDHIYPRSLSKKH
                     FGVIFNSEVNLIYCSSQGNREKKEEHYLLEHLSPLYLKHQFGTDNVSDIKNFISQNVA
                     NIKKYISFHLLTPEQQKAARHALFLDYDDEAFKTITKFLMSQQKARVNGTQKFLGKQI
                     MEFLSTLADSKQLQLEFSIKQITAEEVHDHRELLSKQEPKLVKSRQQSFPSHAIDATL
                     TMSIGLKEFPQFSQELDNSWFINHLMPDEVHLNPVRSKEKYNKPNISSTPLFKDSLYA
                     ERFIPVWVKGETFAIGFSEKDLFEIKPSNKEKLFTLLKTYSTKNPGESLQELQAKSKA
                     KWLYFPINKTLALEFLHHYFHKEIVTPDDTTVCHFINSLRYYTKKESITVKILKEPMP
                     VLSVKFESSKKNVLGSFKHTIALPATKDWERLFNHPNFLALKANPAPNPKEFNEFIRK
                     YFLSDNNPNSDIPNNGHNIKPQKHKAVRKVFSLPVIPGNAGTMMRIRRKDNKGQPLYQ
                     LQTIDDTPSMGIQINEDRLVKQEVLMDAYKTRNLSTIDGINNSEGQAYATFDNWLTLP
                     VSTFKPEIIKLEMKPHSKTRRYIRITQSLADFIKTIDEALMIKPSDSIDDPLNMPNEI
                     VCKNKLFGNELKPRDGKMKIVSTGKIVTYEFESDSTPQWIQTLYVTQLKKQP"
     gene            4420..5412
                     /gene="cas1"
                     /locus_tag="ACF2SP_002580"
     CDS             4420..5412
                     /gene="cas1"
                     /locus_tag="ACF2SP_002580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HFD2360353.1"
                     /translation="MKHLTLSEQGSFCKLQGERILVFNKDQMVFEGTLNRLKTISIVK
                     QGVTLTSNLLVACAMRGIQIFILDYSGKAVCSLQGGLHRTAKVRESQFKYIYTKEASN
                     MAARFILAKIKKQSAVLKYFAKAKNRPENEKIILHAASQKMAGIVANITETNWIKLQD
                     WRFTLLGFEGSAARIYWETVKEVNLVCSDFEGRRPRVNKDITNSMLNYAYSILSSWVW
                     QAITNAGLELYAGILHTSKPGKPSLVLDLMEEFRPWCADRIIFKLHARAQKQNELTPA
                     LKKTLIGEMANLYAKPLPYHKRRISLESIIQRQVYHLSAVFSGEGKYKGYLFKW"
     gene            5419..5718
                     /gene="cas2"
                     /locus_tag="ACF2SP_002581"
     CDS             5419..5718
                     /gene="cas2"
                     /locus_tag="ACF2SP_002581"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFD2360354.1"
                     /translation="MQIRLYKEYIVSYDIRDNSIRRTIYERLKDIGLTPVQKSVFWGY
                     LNEPEKRYLTNFLKKELAKQDTAFITPCKLAQEYEVNLINLELDELRKPDGHIIV"
     gene            5699..6292
                     /gene="cas4"
                     /locus_tag="ACF2SP_002582"
     CDS             5699..6292
                     /gene="cas4"
                     /locus_tag="ACF2SP_002582"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFD2360355.1"
                     /translation="MDTSSYKLPVNMIRQYCFCPRIVYYHMVVGLKVAYPGWVQYGEE
                     YQHKQQVLDKRRTLSKFKPISEQAVLRQNTMLEHPSYPFYGICDGIIEDELEIIPIEL
                     KSFASKISLGQKMQLTAYGMLSELIFEKPCTRGYIITGEKAKAKLIKIDEKSKFKVIE
                     ICAKIKKIFGTETLPYSTANGLQCIQCEFLNHCNDRF"
BASE COUNT         2340 a         1308 c         1111 g         1736 t
ORIGIN      
        1 ctgaggcatt tactataacc atatttggat atagtgctcc aacctcagat atcgaggccg
       61 ttagtctttt gaataaagtg tggcttcaca tttcagaacg ccaatttgaa catgttgaag
      121 taattgatat tcttatgtcg agctgaggtt tttattaaca actgccccaa tagagccaag
      181 ttcactgcta aaaaacatga gccaaattta tttttagtta tttttatcag tcctattttt
      241 gaaattccaa agtatcaaga ctatatttat tttaacgaat catacagcta caaggatgta
      301 gtaaatggaa tcttctcaga ttttatcccc tataggcata gatttgggcg gcaaatttac
      361 aggggtttgc ttatcccacc tcgaagcttt cgcagaactc ccaaaccatg caaatacaaa
      421 atactctgtc atcctgatag accataacaa ctttcaatta tcccaggcac aaagaagggc
      481 aacaaggcat agggtcagaa ataaaaagag gaatcaattt gtaaaacgag ttgccctgca
      541 attattccaa cacattcttt cacgagatct aaatgcaaag gaagagacag cactctgtca
      601 ctaccttaat aaccgaggat acacttatgt agatacagat ttggatgaat atataaaaga
      661 cgagactacg attaatttat taaaggaatt actgccctca gagtcagaac ataatttcat
      721 tgattggttt ctgcaaaaga tgcaatccag tgagtttagg aaaatattgg tatctaaagt
      781 agaagaaaaa aaagatgata aagaattaaa aaatgctgta aaaaacatca aaaatttcat
      841 tactggcttt gaaaaaaaca gtgttgaagg ccatagacac cggaaagtgt attttgaaaa
      901 catcaaatca gacatcacaa aagataacca acttgatagc ataaaaaaga aaatcccttc
      961 tgtttgtctc tctaacctgc ttggacacct aagtaacttg cagtggaaaa acctgcaccg
     1021 ctatctggca aaaaacccca aacaattcga tgaacaaact tttggtaatg aatttcttag
     1081 aatgcttaaa aacttcagac atcttaaagg aagccaagaa tctttagccg ttcgcaacct
     1141 aatacaacaa cttgagcaat cccaagacta tatatccatt cttgaaaaga caccacctga
     1201 aattactatt cccccctatg aggcccgaac aaacacaggt atggaaaaag accaaagtct
     1261 attacttaac cctgaaaaac ttaataacct ttatccaaac tggcgcaatt taataccagg
     1321 aatcattgat gcccaccctt tccttgaaaa ggacttagaa cataccaaat tgcgtgatag
     1381 aaaacgcata attagcccgt ctaaacaaga tgagaaacga gactcttaca ttttacagcg
     1441 atatttagac ctcaataaaa aaatcgataa atttaaaatt aaaaagcaac tctcgtttct
     1501 tggccagggg aaacaactac cagccaattt aatagaaaca cagaaggaaa tggaaactca
     1561 tttcaactct tcattagtga gtgttcttat ccaaatagcc agtgcttata acaaagagag
     1621 agaagatgct gcccaaggga tttggtttga taatgcattc agtctttgtg agctatcgaa
     1681 tattaatcca ccacgcaaac aaaaaatact accacttctt gttggagcga tactcagcga
     1741 agatttcatc aacaacaaag ataagtgggc taaattcaag attttttgga acacccataa
     1801 aatcggacgc acttcattaa aaagcaaatg taaggaaatc gaagaagcac gtaaaaactc
     1861 aggtaacgca tttaaaattg attatgaaga agccctaaac catcctgaac atagcaacaa
     1921 taaagctctc atcaagataa ttcaaacaat accagatatc attcaggcca tacaaagtca
     1981 tcttggacat aatgactcgc aggccttgat atatcacaac ccatttagtc tttcgcagtt
     2041 gtatacaatc cttgaaacca agcgagatgg ttttcataaa aattgtgtag cggttacttg
     2101 tgagaactat tggagatctc aaaaaacaga aattgacccc gagatttcat atgcttcaag
     2161 gttgcctgcc gacagcgtaa ggccttttga tggtgtactg gccaggatga tgcaacgcct
     2221 ggcttatgaa attgcaatgg ccaaatggga acaaataaaa cacattcccg ataactcaag
     2281 ccttttaatt ccgatctacc ttgaacaaaa ccgttttgaa tttgaagagt ccttcaaaaa
     2341 aatcaaaggg agcagcagtg ataagactct tgaacaagcc atcgaaaagc aaaacatcca
     2401 gtgggaagag aaatttcaac gcattattaa tgcctccatg aatatttgtc catataaggg
     2461 cgcatcaata ggcgggcaag gagaaattga ccatatttat ccgcgctcgc tttcaaaaaa
     2521 acattttgga gtaattttta atagcgaagt caatctcatt tactgttctt cccaaggaaa
     2581 tcgagagaaa aaagaggaac attatcttct tgaacatcta agcccacttt atttaaagca
     2641 ccaatttggc actgataatg ttagcgacat caaaaatttt atttctcaaa acgttgccaa
     2701 cattaaaaaa tatattagtt ttcatttgct tacgcctgaa cagcaaaaag cggctaggca
     2761 tgccctcttt ctagattatg acgatgaagc attcaaaacg atcactaagt ttttaatgtc
     2821 ccaacaaaaa gcgcgtgtca atggaaccca aaaattttta ggaaaacaaa tcatggagtt
     2881 tcttagcacc ttggcggatt ccaaacagct ccaattggaa ttttccataa aacaaatcac
     2941 tgccgaagaa gtacacgatc atcgtgagtt attgtccaaa caggaaccaa aattagtcaa
     3001 aagtcggcaa caatcgttcc caagccatgc gatagatgca acgttgacaa tgagcatagg
     3061 tcttaaagag ttcccgcaat ttagtcagga acttgataat tcctggttca tcaaccacct
     3121 aatgcctgat gaggtacatt taaatcccgt tcgatccaaa gaaaaataca acaaacccaa
     3181 catcagcagc acaccgctgt ttaaggattc tctttatgcc gaacgattca tccctgtttg
     3241 ggttaaaggc gaaacatttg ctattggttt ctcagaaaaa gatttgtttg aaataaagcc
     3301 atccaataag gaaaaattgt ttacccttct taaaacctat tccactaaaa atcctggcga
     3361 atcgttacag gaattacagg caaaatccaa agccaagtgg ttgtatttcc ctataaataa
     3421 aactcttgct cttgaattct tacatcatta tttccataaa gaaatagtca caccagatga
     3481 cacaaccgtt tgccatttca taaatagcct acgttactac actaaaaagg aatccattac
     3541 tgtgaagata ttaaaagagc ctatgcctgt tctgagcgta aaattcgaat cttccaagaa
     3601 aaatgtttta ggctctttta aacatacaat tgctttgcca gctaccaaag attgggagag
     3661 actctttaac catccaaact ttcttgcact aaaagccaac cctgccccca acccaaaaga
     3721 gtttaacgaa tttatcagga agtattttct ttctgacaat aacccaaatt cggacattcc
     3781 aaacaatgga cacaacatta agccacaaaa gcacaaagcc gttcgaaaag tattctcact
     3841 gccagtaata cctggtaatg caggcaccat gatgcgtata aggcgcaaag ataataaagg
     3901 acaaccctta taccaactgc agaccattga tgacacccct agtatgggaa ttcaaattaa
     3961 cgaagacagg ctagtcaaac aagaagtact tatggatgct tataaaacca gaaatttatc
     4021 aacaattgac ggaattaata actccgaggg acaagcgtat gctactttcg ataattggct
     4081 aactctgcct gtttcaacct tcaaaccaga aattataaaa ctcgaaatga agccccactc
     4141 caaaaccagg cgctatatac gaattaccca atctttagct gattttatta aaaccattga
     4201 cgaggcttta atgattaaac cctccgattc aattgatgat cccttaaaca tgcccaatga
     4261 aattgtttgt aaaaacaaat tattcggtaa tgaattaaaa ccacgtgatg ggaaaatgaa
     4321 aattgtatca actggaaaaa ttgtgactta tgagtttgaa tccgattcca caccccaatg
     4381 gatacagaca ctctatgtca ctcaactgaa aaaacaacca tgaaacacct cacgctctct
     4441 gaacaaggca gcttttgtaa gttgcaaggc gaaagaatcc ttgtctttaa taaagatcaa
     4501 atggtttttg aaggaacgct caaccgttta aaaaccataa gcatcgtcaa gcaaggcgtc
     4561 actttgacct caaatcttct tgttgcctgc gcaatgagag gaatacaaat tttcattctg
     4621 gattattcgg gtaaagcagt ttgtagcctt cagggtggac tccatcgcac agccaaagta
     4681 cgggaaagcc agttcaaata catatacacc aaagaagcat ccaacatggc tgctcgattc
     4741 attttagcaa aaattaaaaa acagtccgcc gttctgaaat actttgcaaa agctaaaaac
     4801 cggcctgaaa atgagaaaat aattcttcat gcagcatccc agaaaatggc aggtattgtt
     4861 gccaatatta cagagacaaa ttggataaaa cttcaggatt ggcggttcac gctactcgga
     4921 tttgaaggaa gtgctgccag aatatattgg gaaacagtta aagaggtcaa tttggtgtgc
     4981 agtgatttcg aagggaggcg cccaagggta aacaaagaca ttacaaattc catgttaaat
     5041 tatgcctact cgattttgtc cagttgggta tggcaggcca taactaacgc tggattagag
     5101 ctatatgctg gaattttaca tacctccaaa ccagggaaac catcgttagt actcgatctc
     5161 atggaagaat ttcgaccctg gtgcgccgat agaattattt ttaaactcca cgcccgtgcc
     5221 caaaaacaga atgaactaac ccccgctttg aaaaaaacgc taattggtga gatggcaaat
     5281 ttatacgcaa agccactgcc ataccacaaa cgaagaatat cccttgaaag catcattcaa
     5341 agacaagtct atcacctatc agctgtattc tcaggcgagg gaaagtataa aggctactta
     5401 tttaaatggt aatgaaaaat gcaaataaga ttatacaagg aatacatagt ttcctacgac
     5461 atcagggaca acagcatccg acgtacaatt tacgaaaggc ttaaagacat tggcttaacc
     5521 cccgttcaaa aatcagtatt ttggggatat ttaaacgaac ctgaaaaaag atatttaacg
     5581 aactttctaa aaaaagaact tgccaaacaa gataccgctt ttattacacc ttgcaagctg
     5641 gctcaagaat acgaagtaaa cctcattaat cttgaactgg atgaacttag gaaaccggat
     5701 ggacacatca tcgtataaac tgcctgtaaa tatgataaga cagtactgct tttgtccccg
     5761 cattgtttac taccatatgg tggttggcct gaaagtagcc taccctggct gggtgcaata
     5821 cggtgaggaa tatcaacaca agcaacaggt attagacaaa agaagaaccc tttcaaaatt
     5881 caagccaatt tcagaacaag ctgtactcag acaaaatact atgcttgagc acccatctta
     5941 cccattttat gggatttgtg atggaatcat tgaagatgag ctggaaatta tccctattga
     6001 attaaaatct tttgcatcaa aaattagttt aggtcaaaag atgcaactga ccgcctatgg
     6061 aatgctttcc gaactgattt ttgaaaaacc atgtaccaga ggctatatta ttactggtga
     6121 aaaggccaaa gccaaattaa taaaaattga cgaaaaatca aagttcaaag tgattgaaat
     6181 ttgtgctaaa ataaagaaaa tttttggcac agaaacatta ccatacagca ctgctaatgg
     6241 acttcaatgc attcagtgtg agtttctaaa tcactgtaac gatagatttt gaaatcgtta
     6301 tatgctcgct attaagacga gcatagaaaa acgattcaat ctttaatttt gttgatttca
     6361 gggtatcgaa atttagatga tttctaattt aattattgcc ttctttttcg aaaaatcaag
     6421 cctaaataat gcaatttgct atttttcgaa gaaaaccact tttaattcct cgtgtttctt
     6481 taaaataaac aaaga
//
LOCUS       DAXLDT010000161         6463 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXLDT010000161 DAXLDT010000000
VERSION     DAXLDT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.161"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            99..755
                     /locus_tag="ACF2SP_002583"
     CDS             99..755
                     /locus_tag="ACF2SP_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360356.1"
                     /translation="MPQRQSEIVVLKPTNLFLSFLASQLPEANLPSLKLLHTDNTAYV
                     IPKHDSDDGTLNEIEKHFTTMFRHEICRWLGRSAHNEIETSFLDFLCCFKFELHSHII
                     LMEPSLKEGHQMLNIKPRSALLEWMKCAVEDQEGLSDVMSRVNLAQIAENSTVIVKNF
                     TTIKDVKPFIKQYFKPIFETTMSRISGQSVQWPQVNSFQSFSRYFAVEIHTQLIHLHY
                     "
     gene            766..1662
                     /locus_tag="ACF2SP_002584"
     CDS             766..1662
                     /locus_tag="ACF2SP_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360357.1"
                     /translation="MEPVTIALICAASFGAVVALSAFIRQLLLSRDKNLNDEAQRKAI
                     NQEAGELEKMREQMQSNKRFDSHYKVLGANKDAIIYLDNKIEDILRKKAHLVERYAQV
                     SLKESGAIVEGGISTERKDACDRLKKEIDEEIKFYDSELLHLQQRRTSLWDTHTDLQE
                     YLLGQEKSRNESLDHLYKQHTALLEKIYIRHTDDSENIAKQSIDAGTTSFKAIILAPI
                     QFLLQYFNISSGINFDQSKLENAARDDVDKTESDINDSENSDDEIYSDTDANSDDESN
                     RSDNEDSRDESDSENDSRLIFA"
     gene            1878..2216
                     /locus_tag="ACF2SP_002585"
     CDS             1878..2216
                     /locus_tag="ACF2SP_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360358.1"
                     /translation="MVYIKRDKDGRICAVYSQKTSDVTEQIDINDAEFVDFLLRCESD
                     EKLKFLQSDLQLIRVLEDLIDVLIDKNIITITDFPQSVIDKLLARQAFRKRFASRAGM
                     ELNGDDETTT"
     gene            2191..3282
                     /locus_tag="ACF2SP_002586"
     CDS             2191..3282
                     /locus_tag="ACF2SP_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFD2360359.1"
                     /translation="MEMMRQQRNLLLIPFILCFILARPVFSLNLDALLPDERNTVEVF
                     QKASSKVVYVHRLANATVQRRYSLQKTHIPDGAGSGIIWDNKGHVVTNFHVINGADDI
                     AITLGNMTVPAKVIGSEPRKDIAVLEIKSPKALNYLKSFQPFEIVSLNDLIVGQKAIA
                     IGNPFGLDHSLSKGVISALGRKVPGIGGVTIYDMIQTDTPINPGNSGGPLLNSAGQLI
                     GMNTMIYSRSGSSAGIGFAVPAEDIQKIASQIINHGRVVLSGIGIQRVEPHLAERLGV
                     KKGILIADVVPGTPADKLKLRGTHRNQWGRIVLGDVIVGVNAHPVPNYDALYNLLTEI
                     KVGEQITVSIIRNGKKMDVKMKTIDIAAL"
     gene            3402..3947
                     /locus_tag="ACF2SP_002587"
     CDS             3402..3947
                     /locus_tag="ACF2SP_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFD2360360.1"
                     /translation="MLLNRADSLLVLIDVQEKLTPAILNLDLFLARCEWLLKLARKLD
                     VPVLASEQYPKGLGRTVSPLSSYINQEECIEKIHFSCVQEPQYINLLKGYNKSQLILI
                     GIEAHVCVLQTAIEMKGYGFDVFVVVDGVSSRSEFDLKYGLKRMKQNGIHLITAEMVL
                     FEWLKQAGTPEFKAISKEFLQ"
     gene            4157..4900
                     /locus_tag="ACF2SP_002588"
     CDS             4157..4900
                     /locus_tag="ACF2SP_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360361.1"
                     /translation="MNFDNQIALVTGGVSGMGKACVQYLQQHGMKVVVWDKQEGTSNE
                     AELYVACDVTSDESVEKAMRQTISQVGVPRVCINCAGIAPAKRMVGKEGPMPLESFRQ
                     VIDVNLIGTFNVMRIAAHAMSGLELDNKSQERGVIINTASIAAFEGQIGQSAYSASKG
                     GIVSMTLPAARELAQFAIRVNTIAPGLIATPLLLNMPQEVQDSLVATVTFPKRLGRPE
                     EFASLVGHIIENQMINGEVIRLDAALRMR"
     gene            complement(4957..5454)
                     /locus_tag="ACF2SP_002589"
     CDS             complement(4957..5454)
                     /locus_tag="ACF2SP_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella synthesis protein FlgN"
                     /protein_id="HFD2360362.1"
                     /translation="MNNNNKAKTLITQLEQEINWVEELNTLLSEEKEILSTSQFNQLE
                     TLAEKKQELSTKLEASAKQRVDLIKESNPGDKPHGLLLSDFLKNCATEDGIQINHLNN
                     TLAERLNLCRELNTINGQVIANNMYTRQQIVNALSGNKSDAVSVYTSNGNLETPSESS
                     HHEEA"
     gene            complement(5471..5791)
                     /gene="flgM"
                     /locus_tag="ACF2SP_002590"
     CDS             complement(5471..5791)
                     /gene="flgM"
                     /locus_tag="ACF2SP_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFD2360363.1"
                     /translation="MINTIEDIHMVNQINDSANLRHIDMDNRINAKHKEAQNPVLENN
                     SADSVNLSSTSKQLEALKASLKDLPEINEARVLYFKAEIQSGQYEIDSSKIAHGMLNS
                     VEMV"
     gene            complement(5882..>6463)
                     /gene="flgA"
                     /locus_tag="ACF2SP_002591"
     CDS             complement(5882..>6463)
                     /gene="flgA"
                     /locus_tag="ACF2SP_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFD2360364.1"
                     /translation="SNYTEGKIQVTADNIDPRLNLKVCDENKLEVFNPYQTPMLNTST
                     MGIKCLEENNHWTLYIPVKITIFKSVLVAKRALLKGTKISNSDIYQTELDVQKLKQGY
                     FTDSKELIGLVCKHDITPNSPLNPFNIELAKLVHKGEQVSIIAAHDNLTVSMDGIAMD
                     EGALGDSVKVKNLSSKRIIEAQVTGKKTVKVTF"
BASE COUNT         1957 a         1068 c         1348 g         2090 t
ORIGIN      
        1 tagtcaaaat agtgactaag attctaaatt gagatgagac gttgatttta atagttgaga
       61 tgtaatcagg aggagttaat taaatcaagg agagcctcat gcctcaaaga caatctgaga
      121 tagtagtgtt gaagcctact aatttatttt tatcctttct agcgtcacaa ttaccggaag
      181 ctaatttacc cagcttaaaa ttattgcata ctgacaatac ggcttatgtt attcccaagc
      241 atgattctga tgatggtact cttaacgaga tcgaaaagca ttttactaca atgttcagac
      301 atgaaatttg tcgttggttg gggcgttctg cacataatga aattgaaaca agttttcttg
      361 attttctgtg ttgttttaaa tttgagttac attctcatat tattttaatg gaaccctctt
      421 taaaagaagg gcaccagatg ttgaatatca agcctcgttc agcgctttta gagtggatga
      481 aatgtgctgt tgaagatcaa gaaggccttt ctgatgttat gagtcgggtg aatttggctc
      541 agatagcgga aaactcaacg gttattgtta aaaacttcac aactatcaaa gatgtaaagc
      601 catttataaa acagtatttt aagccaatat ttgaaacgac tatgagccgg ataagcggtc
      661 aatcagtaca atggccacaa gtcaattcat ttcaatcttt cagccggtat tttgctgttg
      721 aaatacacac ccaattgata catttgcact attaaggagt tgactatgga accagtgact
      781 atagccctca tctgtgcggc gtcgtttgga gcagttgtag cattatccgc atttatcaga
      841 cagctattat taagtagaga taaaaattta aatgatgaag cacagagaaa agcaataaat
      901 caggaggccg gtgaactcga gaaaatgaga gagcagatgc aaagcaataa acgatttgat
      961 tctcattata aagttcttgg cgcgaataaa gatgcgatta tctacctgga taataaaatt
     1021 gaggatattt tacgtaaaaa agctcactta gtggagcgtt atgcacaagt atctctcaag
     1081 gaatcagggg ccatagttga gggtgggatc tcgacagaaa gaaaggatgc ctgtgacagg
     1141 ttaaagaagg agatagatga agaaataaaa ttctatgata gtgaattgct gcacctgcaa
     1201 caaaggcgaa cttcattgtg ggatactcat acggatttac aagaatattt gttgggtcag
     1261 gaaaagtctc gcaatgagag tctggatcat ctttacaagc aacatacagc acttttagaa
     1321 aaaatatata ttcggcatac tgatgacagt gaaaatattg caaaacaaag tattgatgca
     1381 ggaaccacct catttaaggc gattattttg gcgcctatac aattcttgct gcaatatttt
     1441 aatatttcat caggtattaa ttttgaccaa tccaaattgg agaatgctgc gcgagatgat
     1501 gttgataaaa ctgaaagtga tatcaatgat tctgagaata gcgatgatga gatttatagc
     1561 gataccgatg ccaattctga cgatgagtct aatcgttcag ataatgaaga ctctcgggat
     1621 gagtcggatt ctgaaaatga ttcaagacta atttttgcat aaaaattaat cttttgagtt
     1681 aattggtgac atctatgaaa ggatatatta caatacgatg cttgtactta atgatattcc
     1741 tggaataatt catgaaagtc ttttgtctgg gtttgggctt accattaata cggaaggttt
     1801 gagtttagag acagcagaca taagatttat gtgtctgttt tttctaaaca tagtattagc
     1861 taaaaaagga ttaaacagtg gtctacataa aacgagataa ggatggtcgg atttgtgccg
     1921 tatatagcca gaagacctca gatgtaacgg agcaaattga cattaacgat gcagaatttg
     1981 ttgatttttt gttgcgttgt gagagcgacg aaaaattaaa atttctacaa tccgatttgc
     2041 aattgattcg tgtacttgaa gatttgattg atgttttaat agataaaaat atcattacta
     2101 ttacagattt tccacaatca gtaattgata aattattagc gcgacaggca tttcgcaaga
     2161 gatttgccag ccgtgcaggt atggagttaa atggagatga tgagacaaca acgtaactta
     2221 ttacttatcc cttttatttt atgttttatt ctggcaagac ctgtttttag tttaaacctg
     2281 gatgctctcc ttccagatga acggaataca gttgaagtat ttcaaaaagc ctcatccaaa
     2341 gttgtttatg ttcatcgatt ggcaaatgct actgttcagc gtcgttactc attacaaaaa
     2401 acacatattc ctgatggggc aggctccggt attatttggg ataacaaagg ccatgttgta
     2461 accaattttc atgtcattaa cggtgctgat gacatagcaa tcactttggg aaatatgact
     2521 gttcctgcca aagtgattgg ttcagagcct cgcaaggaca ttgcagtcct ggaaattaaa
     2581 tcacccaagg ctctgaatta tttaaaatca tttcagccct ttgaaatagt ctctcttaat
     2641 gatttgatcg ttgggcaaaa agcgattgcg attgggaatc cttttgggtt agatcatagt
     2701 ctatcaaaag gggtgatctc cgctttaggg agaaaagtcc ctggtatagg cggggtaaca
     2761 atttatgata tgatccagac ggatacccct atcaatccgg gtaattcagg ggggcctcta
     2821 ttaaatagcg caggccaatt aattggtatg aacaccatga tttattcacg atcgggttct
     2881 tctgcaggta ttggtttcgc agttcccgcg gaagatattc agaaaattgc atctcaaatt
     2941 attaatcatg gtcgagtggt attgtctggt atcggtattc aacgagttga gccacatctt
     3001 gctgaaaggt tgggagttaa aaagggtatt ttgattgctg atgtggtgcc tggaactcct
     3061 gcagacaaat tgaagttacg aggtactcac agaaatcaat gggggcgtat tgttttaggg
     3121 gatgttattg taggtgtgaa tgcacatcct gtcccaaatt atgatgctct gtataatttg
     3181 ctcacggaaa ttaaggtggg agagcaaatt actgtttcaa taataaggaa tggtaagaaa
     3241 atggatgtca aaatgaaaac aattgatatt gcagcgcttt aactctcttc attttttcta
     3301 actattattt ccccggaaaa gaataatacg tcaatccggg gttcactcac ttttcttaat
     3361 gctatatgat atagttcaat aaatagaatg aggctatcag gatgttacta aatagagcgg
     3421 attctctttt ggtcttgatt gatgtacagg aaaaactaac cccggcaata ttaaatctcg
     3481 atcttttttt agctcgatgt gaatggttac taaaattagc cagaaaactg gacgtgcctg
     3541 ttctagccag tgaacaatac cctaaaggtt taggtcgaac cgtttctcct ttaagctctt
     3601 atatcaatca agaagaatgc attgaaaaaa ttcatttctc atgcgtgcaa gaaccacaat
     3661 atattaacct cctgaaagga tataataaaa gccagcttat tcttattgga attgaggctc
     3721 atgtatgtgt gcttcaaacg gccatagaaa tgaagggcta tggattcgat gtctttgttg
     3781 ttgtagatgg tgtgagcagc cgctctgagt ttgatttaaa atacggtcta aaaaggatga
     3841 aacagaatgg gatccatttg ataaccgctg aaatggtact gttcgagtgg ttaaaacaag
     3901 caggtacgcc agaatttaaa gcgattagca aagaattttt acaatagaac atttgcatgg
     3961 cctgattgtc tccttttatt gctgtgttgc aaacgtttct gcggtactca tttactgtat
     4021 gcaagcttcc ttcctcaagg cggttttgcg ctttgcctta aaaaaataag caggtttgcc
     4081 aaggggtcta atagaatact gtttttaagt ggaataagaa aattggaaat tgaaaccgta
     4141 gaattttgga gagaaaatga atttcgataa tcagattgca ctagtaactg gtggtgtctc
     4201 aggtatgggt aaggcttgtg tacaatattt acaacagcat ggcatgaaag ttgtggtctg
     4261 ggataaacag gaaggcacct caaatgaagc ggaactgtat gttgcttgtg atgtaaccag
     4321 tgatgaatct gttgaaaagg caatgcgaca aactatctct caggtaggag taccaagggt
     4381 ttgtatcaat tgtgcgggaa ttgctccggc aaaacgcatg gtaggaaaag aagggccaat
     4441 gcctttggaa tcatttagac aagtaataga tgtcaattta ataggtacct tcaatgtcat
     4501 gcgaatagct gcacatgcca tgtcaggatt ggagttggac aacaaatctc aggagcgggg
     4561 tgtcataatc aatacagctt ccattgctgc ttttgaagga caaataggac agtctgctta
     4621 tagtgcgtca aagggtggaa tagtatcaat gaccttgcca gcagcacgtg aattagctca
     4681 atttgcaata agagtaaata caattgcgcc ggggttaata gcaactcctt tacttttaaa
     4741 tatgcctcaa gaagttcagg acagtttggt tgcaacagtg acttttccca aacgcctggg
     4801 tagaccagag gaatttgctt cactagtagg tcatattatt gaaaaccaga tgattaatgg
     4861 agaggtaatt cgtttggatg cggctctccg tatgcgttga gtagctatgc ctggcgcatc
     4921 atactggaga gctattttaa ccaggggtta tatttcttat gcttcttcat ggtgactgct
     4981 ttcggaaggg gtttccaaat tcccgttaga agtgtaaaca ctcacagcgt cagatttatt
     5041 tcccgacaag gcgttgacaa tttgttgacg ggtatacatg ttattcgcta tgacttgacc
     5101 attaatggta tttaattcac gacataggtt taatctctct gcaagagtgt tatttaaatg
     5161 attgatttga atgccatctt ctgtagcgca atttttgaga aaatcagaca acaggagtcc
     5221 atgaggtttg tcgccggggt ttgattcttt tataaggtct acacgttgtt ttgcactagc
     5281 ctctaattta gttgataatt cctgtttttt ctcggctaat gtctccagct gattgaattg
     5341 actggtagaa agtatctctt tttcttcaga taataaagta ttgagttctt ctacccagtt
     5401 tatttcctgt tctaattggg ttattaatgt ttttgcttta ttgttattat tcatcgagtc
     5461 gtcctgcagt ttaaaccatc tctacactat ttaacatgcc atgtgcaatc ttgctgctat
     5521 caatttcata ctggccggat tgaatttcag ctttaaaata caatacacgt gcctcattga
     5581 tttcaggtaa atctttaaga gatgctttta aagcttccag ttgcttcgat gtactactta
     5641 aattgacgct atcagcagag ttattttcca gaacagggtt ttgggcttct ttatgttttg
     5701 cattgatacg attgtccata tcgatatgcc ttaaattggc agagtcatta atttggttaa
     5761 ccatatgaat atcctcaatc gtatttatca tgattatcgg ctccttttgc aatttcttta
     5821 gtaaattaag gttgattttt aatctttttt ccaaattttt tcttaaaaat taaaacatta
     5881 gttaaaaagt aactttcacg gttttctttc ctgtgacttg agcttcaata attcgttttg
     5941 aggaaagatt ttttactttt acagagtctc ctagcgcccc ctcatccatt gcaattccat
     6001 ccatgctcac tgttaaattg tcatgagcgg ctattataga tacctgttct cctttatgga
     6061 ccagtttggc taattcaata ttgaaagggt taagtgggct attaggcgtg atatcatgct
     6121 tgcaaaccaa tccaataagc tctttactgt cagtaaaata tccttgtttc agtttttgaa
     6181 catcaagctc ggtttggtat atatctgaat tactaatttt tgtacctttc agtaaagctc
     6241 gttttgccac aagtacagat ttaaatatag ttattttgac aggtatatat agggtccagt
     6301 gattgttttc ctctaaacat ttgattccca tggtactggt gttgagcatg ggtgtttgat
     6361 aagggttgaa tacttcaagt ttattttcat cacaaacttt taagtttagg cgaggatcaa
     6421 tattatccgc tgtaacctga attttacctt cagtatagtt tga
//
LOCUS       DAXLDT010000162         6444 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXLDT010000162 DAXLDT010000000
VERSION     DAXLDT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.162"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            157..711
                     /locus_tag="ACF2SP_002592"
     CDS             157..711
                     /locus_tag="ACF2SP_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFD2360365.1"
                     /translation="MNLIKIKSKTLFMVLVYSLLSPAYAVSHPKSTVNDTAIQNEILV
                     YINNYRQQHGLAPLKMDNRIVKEAKIHSMDMAKHAIPFGHKYFKKRIDKLHTQIKNSN
                     AGAENVAYNYKNAQDVVKNWLRSPGHKRNIVGNYDLTGVGIARDEKGKIYFTQIFLKT
                     GNSHYASRKPFPSIFSGALFSKKA"
     gene            1105..2238
                     /locus_tag="ACF2SP_002593"
     CDS             1105..2238
                     /locus_tag="ACF2SP_002593"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFD2360366.1"
                     /translation="MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDF
                     ANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPL
                     VKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
                     AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQE
                     QDSETAGGYATETSEEYKKLQAELIDQYAKTADIVISTALIPGRQAPVLLYKKTVEQM
                     KPGSVIVDLATSRGGNCELSVADKIIKHGEITIVGLSNMAGLVPATASDLYSNNLVHL
                     INILAKNPAELNLNPDDEIIQQAVLCHKGTYLPFQGVKEKQNA"
     gene            2231..2527
                     /locus_tag="ACF2SP_002594"
     CDS             2231..2527
                     /locus_tag="ACF2SP_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HFD2360367.1"
                     /translation="MPEIHTLDNPYITILTIFVLACFVGYYVVWKVTPALHTPLMSVT
                     NAISSIIILGALIAAGSELIGCITWLGGIAIFITSINIFGGFVVTQRMLRMYKK"
     gene            2551..3948
                     /locus_tag="ACF2SP_002595"
     CDS             2551..3948
                     /locus_tag="ACF2SP_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HFD2360368.1"
                     /translation="MNSIVPLFYLFAAICFILALKGLASPATSRRGNLLGILGMILAV
                     GSTLMMPTTYHHILLIALIAGGGIIGTIIALKINMTAIPQLVAAFHSLVGMAAVLVAY
                     CAFLSPASFQIGTPGAIATASLIEMSLGLIIGAITFSGSVIAFLKLQGLISGIPIRFY
                     AQNYVNLLIGLTILVLLILFVANQNLFLFNLLAGLAFLIGVLLIIPIGGADMPVVISM
                     LNSYSGWAAAGIGFTLSNHLLVITGALVGASGAILSYIMCVGMNRSIFNVIFGGINSS
                     GNTKQVHSANELRNVRQANGEDAAFLLSNAKDVIIVPGYGMAVAHAQHAVKELVDALE
                     SRSIRVRFAIHPVAGRMPGHMNVLLAEANIPYDRVFEQSEINRDFATCDVAYVIGAND
                     ITNPAAKTDPASPIYGMPVLEVEKAKTVLFVKRSLSPGYAGVENDLFFHDNTYMLFGD
                     AKVMTESIAKALGEI"
     gene            4083..4466
                     /locus_tag="ACF2SP_002596"
     CDS             4083..4466
                     /locus_tag="ACF2SP_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360369.1"
                     /translation="MLRKIGFTLLCAVATFSSSTYSSVTHTLQAGVTLEYEFPPNEPL
                     VFVNYLYWEVAADCKITSEDPSNELFAEALAKAGKINGVTLSKGNTMKVTVHSGDILK
                     LSAESGAKVQITNFGPHTLKATCTA"
     gene            complement(4532..6112)
                     /locus_tag="ACF2SP_002597"
     CDS             complement(4532..6112)
                     /locus_tag="ACF2SP_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFD2360370.1"
                     /translation="MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTV
                     KGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
                     GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIG
                     DSADELREEIELVKGASHEFNLEAYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGP
                     QPRATQERVVSPHEETFSGFVFKIQANMDPKHRDRIAFVRVCSGSYKKGMKLNHLRIG
                     KEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEHLKFTGIPNFAP
                     ELFRLVRLRDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRL
                     KHEYKVDCIYESVNIACARWVYSEDDKAMSEFRTKAYDYLALDGGDMLMYLAPTKVNL
                     TMAEERYPKIKFCATREH"
BASE COUNT         1999 a         1343 c         1196 g         1906 t
ORIGIN      
        1 cacaccataa tatgaaaatt aagaccaaat aaatccaata aaatactttt agtaaatttt
       61 tagttctatc aaccactgga caaaagtgtg ttttttagta accattcaga tcaaaataat
      121 tcctttattc ttaataataa attatgagga tttcctatga atctgataaa aattaaatct
      181 aaaaccctat tcatggtact tgtatacagt ttgctctccc ctgcttatgc agtatcacac
      241 cccaaaagca cagtgaatga caccgccatt caaaatgaaa tattagttta tatcaataat
      301 taccgacaac agcatggcct ggctcctctc aaaatggata accgcatagt aaaagaagca
      361 aaaattcatt ccatggatat ggcaaaacac gcgataccat tcggccataa atattttaaa
      421 aaacgtattg ataaattaca cacacaaatt aaaaactcaa atgcaggggc tgaaaacgtg
      481 gcttacaact ataaaaatgc ccaggatgtt gtcaaaaact ggttacgtag cccaggacac
      541 aaaagaaata tcgttgggaa ctatgacctt accggggtag gtatagctcg agatgaaaag
      601 gggaaaatat attttactca aatattctta aaaacaggaa atagccacta tgcctccaga
      661 aaacccttcc catcaatttt tagtggtgcc ctcttcagta agaaagcata aaaattaaaa
      721 tagggtgttt aacaaaattt ttgcaatttt taagtgcaat tgagtggaat taatgacaac
      781 gttagcctaa tattgaaaca tagacggcaa tcattgttca gccaacagta caattttgga
      841 ggccagattg agagtgtggt gagcaagctc agcctagact gagaagccaa aagcactccg
      901 gaggaatcca cagttttaaa caggactgca ctcaatcttg ctgtctataa catactaaca
      961 ccatggggcg tattcaatac gcccttttta ttggttcatt cctcgcttgt ccggatccaa
     1021 agatccaaaa ttggtatttt atgctgtaga aaattattct aaattacaat aggatagaat
     1081 aataacggta ataaggattt tcccatgatg attgcaacct taatggaatc agcccaggaa
     1141 acgcgagtag ccattactcc caactcggcc aaacaataca taaaactagg ctatgatgta
     1201 gggattgaaa aaaacgcggg gcttagctca gattttgcta acgaagaatt tgaaaaagct
     1261 ggcgcaataa tattcgatag taaaagtaca ttactcaaaa aaacacaaat tcttttatgt
     1321 gttaacgaac ccgatcccaa aaacctgaat ggcttacctc cagattctct aataatcgga
     1381 catatagaca ataacccact tagcccgctt gttaagtggt gtctggatca aaaaatgaca
     1441 ttattttcaa tgaatctcat tcccaggata agtcgtgctc aaagcatgga cagcctatcg
     1501 tctcaggcta acctagctgg ttacagggca gttttggaag ctgcggcaac atttcataga
     1561 gctataccta tgatgatgac agcagcaggc atgatacaac cggccaaagc acttattctt
     1621 ggtgccggag ttgcaggctt gcaagccata gctactgcga aacgtttagg tgcagtggtt
     1681 tatgcctttg acgtaagaaa ggcagccaaa gaacaggttg aaagtttagg cgctgaattc
     1741 atagaggtaa tgcaggagca agatagtgaa acagctgggg gatatgccac agaaacaagc
     1801 gaagaatata aaaaacttca ggctgaactc attgatcaat atgcaaaaac tgcagacatt
     1861 gtaatttcta ctgccttgat accagggcgt caagctccgg ttttacttta taaaaaaact
     1921 gtcgagcaaa tgaaaccagg ttctgtcatt gttgatctcg caacgtctcg aggtggaaat
     1981 tgtgaactca gtgttgccga taaaatcatc aagcatggtg agattactat agtgggatta
     2041 agtaatatgg caggtctcgt ccctgctacg gcaagtgacc tttattctaa taatctggtt
     2101 catcttataa acattttggc gaaaaatccg gctgaactta acttaaatcc tgatgatgag
     2161 attatccagc aagccgttct ttgccataaa ggaacttacc tgccctttca aggagtaaag
     2221 gagaagcaaa atgcctgaaa ttcatacact tgataatcct tatattacaa tattaaccat
     2281 tttcgtactg gcctgttttg taggttatta tgtggtttgg aaagtaacac cggctttaca
     2341 tacaccccta atgtcagtaa ccaatgccat atccagtatt attatacttg gtgctttaat
     2401 tgctgcagga agtgaattga tcggatgcat aacctggtta ggtggcatag ccatattcat
     2461 tacttcaatt aatatttttg gtggctttgt agtaactcaa cgcatgcttc gcatgtataa
     2521 aaaataatga tttataggac ggaatcagta atgaattcaa tagttccact gttttattta
     2581 tttgcagcca tttgctttat attggcttta aaaggactgg ccagtccagc tacttcaaga
     2641 agggggaacc ttctaggcat tctgggtatg atcctggcag tgggctccac cctgatgatg
     2701 cccactacct accatcatat tcttttaatc gccttaatag caggcggcgg aataatagga
     2761 actattattg ctttgaagat caacatgacg gccataccac aactggttgc tgcttttcat
     2821 tctttagtag gcatggcagc agttctggta gcctattgtg cctttttatc ccccgcgtcc
     2881 tttcaaatcg gcacacctgg tgctattgcg accgcaagtc taattgaaat gagcctggga
     2941 ttaattatcg gagccattac cttttctggt tcagtcattg cctttttaaa attgcaagga
     3001 ctcatatcag gtattccaat tcgattttat gctcaaaact atgtcaactt actaatagga
     3061 ctaacaatcc tggtgttact cattttattt gtagccaatc aaaacctgtt tctctttaac
     3121 ctgctcgcgg gattagcttt tctcattggg gttttactga ttattcctat tggcggagcc
     3181 gatatgcccg tcgttatttc catgctgaac tcctactcag gttgggccgc agcaggaata
     3241 ggtttcactc tgagtaatca tctgttagtt atcacaggcg ctctggtagg cgccagcggg
     3301 gcaattttaa gctatatcat gtgcgttggt atgaatcgct ctatttttaa tgtcattttt
     3361 ggtggaataa acagttctgg aaatacgaaa caagttcatt ctgctaatga gttacgcaat
     3421 gtcaggcaag ccaacggcga agatgctgca ttcctattaa gtaatgccaa ggatgtcatc
     3481 attgttcctg gatatggaat ggcagtggca catgctcaac atgcggtgaa agagcttgtt
     3541 gatgccttgg aatctcgttc tattcgtgtt cgctttgcaa tacaccctgt tgctgggcgc
     3601 atgcctggcc atatgaacgt attacttgct gaagccaata tcccctatga cagagtcttt
     3661 gagcaaagtg aaattaacag agattttgct acctgtgacg ttgcttacgt gattggagct
     3721 aacgatatca ccaaccccgc agctaaaact gatcctgcct cacccattta tgggatgcct
     3781 gtcctggagg ttgaaaaagc taaaacggtc ttattcgtca aacgcagctt atctcctggt
     3841 tatgccggtg tagaaaacga tttatttttt cacgacaata cctacatgct tttcggagat
     3901 gcaaaagtaa tgactgaatc aatagctaag gctttgggag aaatataacc ctttcaaaat
     3961 aaaaacaata atctctgacg gggtgggtac ccggttcaat cgacgcaaat gccttttaat
     4021 ttgccaacga agcaaaataa atgtatctta gccagttaat ttttcataat ggagatatat
     4081 ctatgctacg aaaaatcggc tttacgttgt tatgcgcagt ggcaacattt agttcaagca
     4141 cttattccag cgtgactcac acactccaag ctggagtaac gctggaatat gagtttcccc
     4201 ctaatgaacc tctagtcttt gttaattatt tgtattggga agtagcggcc gattgtaaaa
     4261 ttaccagtga agatccatcc aatgaacttt ttgccgaggc actcgcaaaa gccggtaaaa
     4321 ttaatggtgt tacgctctct aaaggaaata ccatgaaagt gactgtccat tccggcgata
     4381 ttttaaaatt aagcgcggaa tctggagcaa aagttcaaat caccaatttt ggaccacata
     4441 ctctcaaagc aacttgtact gcataaagaa ttgagctaat cgcaccccaa caccaaacag
     4501 taatggggtg tgaaaattca aatcctgttc atcaatgttc tcgagtagcg cagaacttaa
     4561 tcttgggata acgttcttct gccattgtca aattcacttt ggtaggcgcc aagtacataa
     4621 gcatatctcc tccgtccaaa gccaaataat cataagcctt ggtacgaaac tcgctcattg
     4681 ctttatcatc ttcagaataa acccatcgag cgcaagctat attgactgat tcataaatac
     4741 aatccacttt atattcatgt ttcagtctat gcgctaccac atcaaattgt aatatcccaa
     4801 cagctcccaa tattagctga ttactgttta atggtctaaa aacctgggta gcaccctctt
     4861 cagataattc gattaatcct ttaagtaagg ctttactctt taatgggtcc ctcaacctga
     4921 caagcctaaa caattcagga gcaaaattag gaattccagt aaactttaag tgttcacctt
     4981 gtgtaaaagt atcaccaata cgaatagtac catgattatg caaaccaata atatcaccag
     5041 ctaaagcaag ttctgtgtgt gatctatcgc ctgccataaa agttaaggca ttggaaattt
     5101 gcacttcttt tcctatgcgt aaatgattta atttcattcc cttcttgtaa gagccagaac
     5161 aaacccgcac aaaggcaatt cggtctctgt gtttgggatc catattggct tgaattttaa
     5221 aaacaaaacc ggaaaatgtt tcttcatgcg gagaaacaac tctctcctga gttgctcgag
     5281 gttgaggtcc tggcgcatac tcgacaaaat cgtccaataa ttctttaata ccaaaattat
     5341 taattgctga accaaagtat accggggtca tttttcctgc aagataagct tccaagttaa
     5401 actcgtggga cgctcctttg actaattcaa tttcttctct gagttcgtcc gcactgtcac
     5461 ctatcaactc atctaattga ggattatcca atcctttaat ctgcatagct tcgacttttt
     5521 ttgcattact tccctgctga tataaataaa taatatcttg atagcggtga taaattccct
     5581 tgaagcgttt acccattcca actggccagg taattggcgc acattgaatg cccaaaacag
     5641 actctacttc atctaacaaa tcaataggtt ctcgcccttc tctgtccaat ttattgataa
     5701 aagtcatgat cggtgtatca cgtaaacggc aaacttccat caattttacc gttcgctcct
     5761 ctactccctt ggccacgtca attaccatta aggcagaatc tacagcagtg agagtacgat
     5821 atgtatcttc agaaaagtct tcatggcctg gtgtatctag caaattgatg acgtgttgat
     5881 tatgtataaa ctgcataaca gacgtggtaa tcgatattcc tctctctttt tccatttcca
     5941 tccaatcaga agtggcatgc ctgtctgctt tacggccttt gaccgtacct gccaactgaa
     6001 tagctcctcc aaaaagtaat aacttttcag ttacagtggt ctttcctgca tcagggtgag
     6061 aaataatggc aaaagttcgc cttttgttaa agtcttgata aaaatcagac atactgctct
     6121 caaattaagt aattaattaa aaataatagg cttcaatcgc atagtatacc atggaactta
     6181 cgaccccata aacgtagcga attaaaatca ttcctgtata ctcaaaatta gaacctgttt
     6241 tcatgcgatc acatgtgatc aaaacctgat ctaatctgtt ccctgccaaa ctggttcttt
     6301 aaattaaagg atttaattta aaaaaggagt ttgctatgag attcaatcat tttattgctc
     6361 cactggtttt actccctgtt tgtgcattaa gccaacccca aaatgacaaa ggagatgtct
     6421 ttttatcaaa aattccaacc agta
//
LOCUS       DAXLDT010000163         6441 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXLDT010000163 DAXLDT010000000
VERSION     DAXLDT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.163"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(1..555)
                     /locus_tag="ACF2SP_002598"
                     /pseudo
     CDS             complement(1..555)
                     /locus_tag="ACF2SP_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(669..1049)
                     /locus_tag="ACF2SP_002599"
                     /pseudo
     CDS             complement(669..1049)
                     /locus_tag="ACF2SP_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611931.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1389..1745
                     /locus_tag="ACF2SP_002600"
     CDS             1389..1745
                     /locus_tag="ACF2SP_002600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360371.1"
                     /translation="MNLSVFSASELFAGADPEKVIQELTMVNKSYGSAISYNRLPAGS
                     TKDLSEHSKYYLNLIDKAIEDVNLSLDDNSKATYSLRFALLQPLLVNLVAAAEVLKNE
                     KTQEQEQKSSHSVRFM"
     gene            complement(1786..2718)
                     /locus_tag="ACF2SP_002601"
     CDS             complement(1786..2718)
                     /locus_tag="ACF2SP_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360372.1"
                     /translation="MKKNQLNSLTNYYPALTRLRNIQDAQELGEMAHTLPWRQADELI
                     ECLFNNEEEFNRLIWSPYDISIVAKKFPKFADKLIDIFISNPEKFKKIIHFSSELGQV
                     VDALNPRVANKLMDFIFCNENKIYKHIIRDSYNLCRFLFHRNLRQYSDRLINHILKDP
                     DYFKLVVGDMGNLLRLAINHPQHADTLINMVIKDKEHFKKLISNQSNWSEQLSHFPKY
                     EKIFANNVPIDENEKNRQLYLANAPHAEIRKNARLFAQAERTHSGQFFFSEAMPRELR
                     IIIAGLTRDSYLCNEEEANQIAQENFSRPMKNSQ"
     gene            complement(3038..3676)
                     /locus_tag="ACF2SP_002602"
     CDS             complement(3038..3676)
                     /locus_tag="ACF2SP_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HFD2360373.1"
                     /translation="MKSSEICPFGPKLQQYWDKRYDYFHRFDEGIQTDSEGLHTVMPE
                     DAALTQAKLFNNAKIVVDGFCGIGGSAIALARTGKKVIAIEMDSVRLAMAKNNARVYG
                     VEHLITFVHGDFFDVAAEIKADAVLIDPPWGWPRYRKIEEFRLQHFNPNGNKLLSYVL
                     DHFNKIVLRAPKTFITSELDQFNVNYEIHSDKLHHSIISISVCITNHSKRKS"
     gene            4338..4823
                     /locus_tag="ACF2SP_002603"
     CDS             4338..4823
                     /locus_tag="ACF2SP_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005772284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2360374.1"
                     /translation="MRYRRAIIPGATYFFTVNLQNRKSQLLIEYIDVLRSAFRNAMCH
                     YPFAIDAIVILPDHLHMIMTLPENDANFSLRWNLIKGIFSRQLPIKESISPIQKNRRE
                     RGIWQKRFWEHLIRDDLDFEHHVNYIHYNPVKHGYVVHPIDWPYSSVHQFIRNGILLP
                     Q"
     gene            complement(4792..5028)
                     /locus_tag="ACF2SP_002604"
     CDS             complement(4792..5028)
                     /locus_tag="ACF2SP_002604"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFD2360375.1"
                     /translation="MLFNLWYDMPHRPTKDIDLLGFGDNDLAYIQLTFREICSLSASV
                     GRALRPTFLFISPQIQSNEIALQPTTEEEEYHSE"
     gene            complement(5192..5800)
                     /locus_tag="ACF2SP_002605"
     CDS             complement(5192..5800)
                     /locus_tag="ACF2SP_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiEi antitoxin N-terminal domain-containing
                     protein"
                     /protein_id="HFD2360376.1"
                     /translation="MNTKNTHSQKALNLAKEKGIVRSSEFVEEGIPRIVISRLVANQQ
                     LQQIERGLYCLPEKEFSEKESLLVIATRVPQAIFCLFTALQVHGLTTQLPRKIWIAMP
                     IDSRVPKIDYPPIKMVKYSGEAYSQGIETQEADGVSFRVYNVAKTIADCFKHRNKIGI
                     DVAIEALKDAYAQNKVTADELWHYAKICRVAKVMRSYLEAIQ"
     gene            complement(5984..>6441)
                     /locus_tag="ACF2SP_002606"
     CDS             complement(5984..>6441)
                     /locus_tag="ACF2SP_002606"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360377.1"
                     /translation="AMPPVEVIQSCKNNNPANKSVLMTEIEGIGYNKDSEPNCKNQFD
                     TEIDGKLYGSATCDDKPYLIIAGHKIKVESANNYSTNPAIKPTTAIAQVAGWWNILDS
                     KKQTYLCIESPLSNTGTVSNAYQYFIIADPFNEKKSNFDISFYYFDKKD"
BASE COUNT         1997 a         1228 c         1160 g         2056 t
ORIGIN      
        1 ttactcccct ccccaaatcc tatccgtaaa cttatacaac ctgggcgtac ccaccccttt
       61 aatccgaggc ttatgcgttg ccttaccatc aggagccgga ctaatccagc ccacattgag
      121 aagaactcgt gttaccattt tttgctcgaa gtctttgcat agttcatttt tataagcctc
      181 cggtagcacc ataaactggc gatatcctgc atcatcgact tcataaaacc ctgctcggtt
      241 ttttatgtat tcgctgttgg gcgctttaat attgtcaaaa cgactggtac cataagtttc
      301 gaagaatgcg cgtacttggg agagtatggc tctgtcttct cgattacctt ctgtgccaaa
      361 ggtttcttgc caggattcaa aacatttttt tgcagcactg aaggactcgc cactttgcca
      421 tccggttagg ccaaactggg tggcgagttc tcctgctgtt gctactaggg caaatcgtct
      481 agcaacgcgt attgtttgac ctgtaggttc ttgtcctact accttatcaa caaattgctt
      541 aatgatattg cttataacag gaacggaata agggtcagat cttgcctttt gcctctatac
      601 tcaagaatta caaaagttat aaatcaacac aatgggagca aagggcaaga tctgcccctt
      661 acaacacagt cggaatgcac gataaggcct gcttttggct ttctctgcca aatggccata
      721 gttaatgcat cggaaactgt accagccttc attctagaac tcatgttcca accaaccact
      781 tttcggaaaa acaaatcaat tatcaccgtt aggtataacc agcactcatg ggttgggata
      841 taggtaatat cggacacata ggcacgattg ggttcattga cttgaaactt cctgtttaaa
      901 acattctcaa aaacaggttg tttgtgattg ctgtttgttg tcactgtgta ttacttttta
      961 tatcgaacaa aaacacctga ctcacgcatc aaactatggg tttttcttcg gccaaccgga
     1021 taaccaagag cattcagggc tttccttaaa ggtacagttg atacctaatt acggttttat
     1081 ttatgctgtc acaactctat gaaaatgtca cattcagtta ccttatcagt tcgtgaagaa
     1141 ctaaaaagta atctggttgt atgggagtgc tgttgataaa taagcaagag cgccaaagaa
     1201 aagctgtgtt agagatggta aggcaaagac agattagtct ggtgcaaatg tcacagcaat
     1261 ggattcgata tttttatact ataatttatt taaaatcaaa cggagaatgt catgcctttt
     1321 caacttcctt ttaaaaaaag aaaaacccat taaatccaga gaggctctgt ccttcggata
     1381 tttgtttcgt gaacctgtca gtattttctg cttcggaatt attcgcaggg gcagacccgg
     1441 aaaaggtcat acaagaatta actatggtaa acaaatcata tggctcagca ataagctata
     1501 atagattgcc cgctggctct acaaaagatc tgtcagagca ttcaaaatat taccttaatc
     1561 ttatcgataa ggctattgag gatgtgaatt taagtctgga tgacaatagc aaggcaacgt
     1621 attctctcag atttgctcta ttacaaccat tacttgtgaa cctggtagcg gctgcggaag
     1681 tattgaaaaa cgaaaaaact caagaacagg aacaaaaatc aagtcattct gtaagattca
     1741 tgtaattcct actttttcat ccagagttgg ggaatccata gaaaattatt gggaattttt
     1801 cattggacga ctaaaatttt cttgcgcaat ttgattagcc tcctcttcat tacataaata
     1861 ggagtctcga gttaagccag caattattat gcgtaattct ctaggcatgg cctcgctaaa
     1921 aaagaattgt ccactatgag tgcgttccgc ttgagcaaat aaacgcgcgt tttttctaat
     1981 ttctgcatgg ggagcatttg ccaaatacaa ttggcggttt ttttcatttt catcaatagg
     2041 tacattgttg gcaaatattt tttcgtactt gggaaaatga ctgagctgtt cagaccaatt
     2101 agattgatta cttataagtt ttttaaaatg ctctttatcc ttaataacca tattaattaa
     2161 tgtatccgca tgttgaggat gattaattgc aaggcgaagt aaattaccca tatctccaac
     2221 tactagctta aagtagtcag gatctttgag aatatgatta attaacctat ctgaatattg
     2281 acgtaaattt ctatggaaaa gaaagcgaca aagattgtat gaatctctta tgatatgttt
     2341 gtatatctta ttttcattac aaaaaataaa atccattaac ttattggcta cacgtggatt
     2401 gagggcatct acaacttgac ccaactcaga tgagaaatga ataatcttct taaatttttc
     2461 tgggttacta ataaaaatat caatcagttt atcggcgaat ttagggaatt tttttgcaac
     2521 aatgcttatg tcatacggtg accatattaa acgattaaac tcctcttcat tattaaaaag
     2581 acattcaatt aactcatcag cctgtcgcca aggaagagta tgagccattt cacctaattc
     2641 ctgggcatcc tggatattcc taagacgagt caatgctgga taatagttgg taaggctatt
     2701 taattgattt tttttcatgg cataaatctc tcaaactgaa ttgaataata aatacataaa
     2761 ttagtcaaaa aaaataccat gtgcttgctt gtttgagaat tggtgatttt agaaataatt
     2821 aatttccaaa ttaatatatt ctataaaagt tctaaatgaa taatatatag acttaaattg
     2881 gggtaattat atggaaaata aaccaaaagg actaaatttc ttaactggat tgttcaatgt
     2941 atagtacacc aatcccccaa taatgaaggg agtattattg ggacttgggt tggacagcaa
     3001 gcaatgatat ggactcatct ggtatttaaa aacactatca agacttcctt ttggaatggt
     3061 tagtgatgca tacagaaata gaaatgattg aatgatgaag cttatcagaa tgaatctcat
     3121 agttgacatt aaattgatct aattctgaag tgataaatgt ttttggtgca cgtaatacaa
     3181 ttttattgaa atgatcaagc acataactga gtaatttatt tccattcgga ttaaaatgct
     3241 gcaaacgaaa ttcttctatc tttctgtaac gaggccatcc ccatggcggg tcgataagga
     3301 cagcatcagc ttttatctca gcagcaacat cgaaaaaatc tccatgaaca aacgttataa
     3361 gatgttcaac accataaaca cgagcattat ttttagccat tgctaaacgg acggaatcca
     3421 tttcgatagc aatgactttt ttacctgttc ttgccagagc aatagccgac ccgccaatac
     3481 cacaaaaacc atctactaca atcttcgcat tattgaataa tttggcttga gtgagcgcag
     3541 catcttcagg cataacggta tgcaaacctt cagagtctgt ttgaatcccc tcatcaaagc
     3601 gatgaaaata atcgtatcgc ttatcccaat attgttgtaa tttagggcca aatgggcaaa
     3661 tttcagatga cttcattttt tacctgtatc aaagtgcacc ccatatagca agcctgcctg
     3721 cataatcgag gattgaaaat tttagagttt ccttgttgtt tgtatggaat agagttgttt
     3781 gattgacgtt ctgcatggag gatacctcaa tattgttata tttaagtatc cctcgacaca
     3841 tagccactta ttacatcagc accggaggga tcgagagttg ctgattaagc taactattaa
     3901 gctttgatag gctatcgtgc cttgtaacat aattcaagta gagaacaggt atttattttg
     3961 tggatgcaac agctattgac gtaaaacatt taaatttgaa cggattgcaa aaaagggaaa
     4021 atcgtcaata gggtagtttt ttgtcttcaa gcctcatctg gtaatttatg attatggcga
     4081 gttaatagct tttaagctga acggagtcac tgtgtatgac catataccta ttgaggctat
     4141 cagtcagtta atgagcagct taaaaagccc tcactcgaca ttgctcggca agactcattc
     4201 ttgccaagcg cttatttact ataatttgaa aagcaagcgt aggtcgggcg cttcgcccga
     4261 catttccttt ttttcttcct cactaaaatt aaaactgtta tatacactgt cattacggac
     4321 tatataaaca atccaacatg cgctatcgaa gagccattat tcccggggcc acttatttct
     4381 tcactgttaa tctgcaaaat agaaaaagtc aattgctcat cgagtatatt gatgtactac
     4441 gttcggcttt tcgcaatgca atgtgtcatt acccatttgc aattgatgct attgtcatat
     4501 tgccagacca cctgcacatg attatgaccc tacctgaaaa tgatgcgaat ttttcattgc
     4561 gttggaatct aataaaaggc atcttctcaa gacaactacc aataaaggaa tccatttctc
     4621 caatacaaaa aaataggcga gaacggggca tttggcaaaa acgtttttgg gaacatctca
     4681 ttcgtgatga tttagatttt gaacatcatg tcaattacat tcattataat cctgttaaac
     4741 atggttatgt ggtacatcca atagattggc cttattcaag cgttcatcaa tttattcgga
     4801 atggtattct tcttcctcag tagtcggctg taacgcaatc tcatttgatt gaatttgggg
     4861 agaaataaaa agaaatgtcg ggcgaagcgc ccgacctacg cttgctgaaa gactgcaaat
     4921 ttccctgaac gttagctgga tataagccag atcattatca ccaaacccta ataagtcaat
     4981 atccttggtt gggcggtgcg gcatgtcata ccaaagatta aacagcaaag caccttttag
     5041 taaaaattga ctggcgtatt cagatatgcc aattcggtat aacagtcgct cgagaccata
     5101 acgggttaag acagcaataa aatctgactt tgatccttca gcttttgcta ttttttaagt
     5161 cgtgctcgaa tggaagcact tatatctttt gtcattggat agcctctaga taagaacgca
     5221 ttacctttgc aacacgacaa atttttgcat aatgccatag ttcatcagca gtcactttat
     5281 tttgggcata ggcatctttc aaagcttcaa ttgctacatc aataccaatt ttattcctat
     5341 gcttaaagca atcagcaatt gttttagcaa cattatatac acgaaatgaa acaccatctg
     5401 cctcttgcgt ttcaatcccc tgagaataag cttctcctga atacttcacc atttttatag
     5461 gtggataatc tattttaggt acacgactat caataggcat ggctatccaa atctttcttg
     5521 gcaattgtgt agtaagtcca tgaacttgaa gtgctgtaaa aagacaaaaa atggcttgtg
     5581 gcactcgagt ggcaataacc aacaaactct ccttttcaga gaattccttt tcaggaagac
     5641 aataaagccc cctctcaatc tgttgcaatt gttgatttgc aactagacga gatatgacga
     5701 ttcttggaat gccttcctct acaaactcag aggaacgtac gattcctttt tctttagcaa
     5761 gattcagagc cttttgacta tgggtgtttt tagtattcat gcttatagta tgttccgttt
     5821 tttccccatt tgcaaataaa tagggtaaaa acggaacatt gaatatttat aactcagcag
     5881 tatccatcat gtgtccctgc aaacccagtg gtgataaggg tttcccactt gtcccctttg
     5941 tcccagatat attttatatt tctgcattta acaaaaagat tgattaatct tttttatcaa
     6001 aataataaaa tgagatgtca aaatttgatt ttttttcatt aaagggatct gcgatgataa
     6061 aatactgata agcattggat acagtacccg tattagatag tggggattct atgcatagat
     6121 aagtttgttt ttttgaatct aatatattcc accatcccgc aacctgagca atagcggttg
     6181 ttggtttgat agctgggtta gtggaataat tattagctga ttccacttta atcttatgac
     6241 ctgcaatgat taagtagggt ttatcatcac atgttgctga tccataaagt ttaccatcaa
     6301 tttccgtatc aaactgattt ttgcaattag gctctgaatc tttattatag ccaatacctt
     6361 caatttcagt cataagaaca gatttatttg ctggattatt gtttttacat gattgaataa
     6421 cttcaacggg tggcatcgca t
//
LOCUS       DAXLDT010000164         6438 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXLDT010000164 DAXLDT010000000
VERSION     DAXLDT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.164"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..207)
                     /locus_tag="ACF2SP_002607"
     CDS             complement(<1..207)
                     /locus_tag="ACF2SP_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFD2360378.1"
                     /translation="MTNIKLCKIKLLNKIYEIKCPDGEEVSLILAAEKLNEKMQNNKN
                     KFKHLDNFHILLLAALDIGHELIVC"
     gene            363..944
                     /locus_tag="ACF2SP_002608"
     CDS             363..944
                     /locus_tag="ACF2SP_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFD2360379.1"
                     /translation="MSLDNDSLHLPKYDDFVQSISVLALTMSGSELHGIMCGYLCAGA
                     DSQGEAYIRALLNNKKDEQSRNALLSMFSVFSISQQQINNFDFEFEMLLPDDDESLVT
                     RAQAFSEWCEGFTQGLTIAGVGMEQFYEEESQDALQHLIEFAELDCESLEVGEEDERA
                     LMEVSEYTRMAVLRLHSDLVLHERELGDSGTTH"
     gene            1048..2358
                     /gene="pepP"
                     /locus_tag="ACF2SP_002609"
     CDS             1048..2358
                     /gene="pepP"
                     /locus_tag="ACF2SP_002609"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFD2360380.1"
                     /translation="MISQQEYKARRKKLAAQLPDDSVAVIPAAHEVLRSGDAHYRFRQ
                     DSDFYYLTGFNEPDALLVIIAGKDSQSILFNRPRNPAEEQWTGKRLGQDGALQELGVQ
                     AAYPLSSIAEELPKLFSGKSAIYYDLGRNLSLEKKIRHVLSSLKAQVRRGIKAPESLC
                     DLEPILSEMRLLKSDAELELMRQAARISVAAHKRAMQVCQKMEYEYQLEAELIYEFSR
                     HGCRSVAYDPIVGGGANACILHYTENNKPLRRGDLVLIDAGGEYGSYAADITRTFPVS
                     GTFSPEQKIIYELVLKAQKAGIAAIEPGLPWNHIQQTIIRILTEGLCDIGILKGDLEE
                     LIKNEAYKPFYMHNSGHWLGLDVHDVGRYKINNEWRPLEAGMVLTVEPGLYISAGMEG
                     VDKRWWDIGVRIEDDILVTRAGYEVLTGDLPVNVDDIEALMRGE"
     gene            2348..3550
                     /gene="ubiH"
                     /locus_tag="ACF2SP_002610"
                     /gene_synonym="visB"
     CDS             2348..3550
                     /gene="ubiH"
                     /locus_tag="ACF2SP_002610"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFD2360381.1"
                     /translation="MANKETDILIIGGGLTGASLSLALQGLGFSVTLVESRPYSDKVN
                     PDFDARSLALSPASQRILAMLGVWEILKEYTTPIHMIHVSDQHRFGVSRLHGEKNSPL
                     GYVVEMQYINRALSILINKEHLLAPAALKSIDFNNSTATVAHGDDMISIQAQLIVAAD
                     GTESMTRHLCNLPVKIKDYAQDAIVANVGLIKPHGNRAFERFTPNGPLALLPMMNDKM
                     SLVWASKPEEAKRLVMLNDGDFLRELQTAFGYRLGRFGKVGKRYSYPLKQVLMPQQTK
                     WPVVFVGNAAHTLHPVAGQGFNLGLRDIAALAQCISQKGLNPQMLEHYLKLRAHDQQM
                     ITWLTDGLIHLFTSRLPGMGVARNLGLLALDNIPALKNLLARYARGFGGITPDLVCEI
                     ALSTGEFQ"
     gene            3547..4710
                     /locus_tag="ACF2SP_002611"
     CDS             3547..4710
                     /locus_tag="ACF2SP_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HFD2360382.1"
                     /translation="MSQHFDVLVIGGGIVGLTAALAMAQRHYTVAVIDAGTLMVNTDK
                     SDLRVYAINKASKMLLSELGVWQHLDDARVAPYNKMHVWDGVSGAYIDFDSRSIAEPN
                     LGAIIEESILKQALLRRISSESKINLFPQSVIDEVCHLERSIKISGQNTAWEGQLLMI
                     ADGANSPMRHKLKVDVTSWSYEQQALVATVNSEKSHQNTAFQVFHPDGPLAFLPLSNL
                     NQCSIVWSTHADNAMRLKLLDDNEFSRELTRAFGGQLGQIKVESDRHQFPLQMRHVKQ
                     YAGHRWLLLGDAAHTIHPLAGLGLNVGLADVKSWLTCLEMGKGELISKKVLGAYQRER
                     KHSVWQIILLMEGFKRLFGTSASPVTSLRSLGLRACNEFIPIKRLFMRQASGL"
     gene            4886..5653
                     /locus_tag="ACF2SP_002612"
     CDS             4886..5653
                     /locus_tag="ACF2SP_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360383.1"
                     /translation="MTRLKFFSKFQKALLQLETNVSNLSNPLVGSKIKELQTKKILVR
                     ADGRSLDHLHKLGGLPQRVESEKLEKVRITDVERYQKFNMNPFGWGACASAEDLRKFL
                     ENYSELTKQAWVHKFYGSSTSLLTLKSEVGISCGDHDEEKEELVVDSVSFEQIIASTC
                     PKYREKYLGGGLVPNAMKDFKSKVPHTMRLGDFSSEKSTLEWLLINDCEEEFAKLCKE
                     IYGDTPLKILLMRFEGDKYLADAVTYYVKEAASSPRV"
     gene            complement(5643..>6438)
                     /locus_tag="ACF2SP_002613"
     CDS             complement(5643..>6438)
                     /locus_tag="ACF2SP_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2360384.1"
                     /translation="IDSPLFSHLHDGLPIHFDDAKKELGRYDTNNVMARIRLGFPYLA
                     RFSSGGFYPSFSKSNAIIFLSEAYFRYQLEDVSLLLASVNQTAKETGKAAFLKATAVG
                     MGFFAKIDCGYDIQHMIFPYYLRAYKKLLSEHKFPWIAKIEFPIFNEIQQEQFDSIFE
                     DYNGSTKVYQSTRDVLEFREDEIEKYFPAAINPSDAFALTGNEWGYGSVESMIGNNSS
                     IRFDQVHHMNPLILDPSHHVEVQINKDHRVELSANAMPRLSSSIKP"
BASE COUNT         1901 a         1104 c         1468 g         1965 t
ORIGIN      
        1 acatacaatt aattcatggc caatgtccag agcagcaagt aaaaggatgt gaaaattatc
       61 caggtgttta aatttatttt tgttattttg cattttttca ttaagttttt ctgctgcgag
      121 tattaaactc acttcttcgc catcagggca tttgatttca tagattttat ttaataattt
      181 tattttacag agtttaatat tggtcattat tacgcctttg tatttgcacg gtactaagca
      241 aatagtagca attttgcctt aatgcagcaa attatgaaaa tcataggtgc tgagaaagtg
      301 cagcaggtga acactgattc taaatggagt atcattagct atctttttaa atagggttaa
      361 atatgtcttt agataatgat tcgctgcact tacctaagta cgatgatttt gtgcaatcca
      421 tttctgtttt agcattaact atgtccggaa gcgaattaca tggaatcatg tgcggttatc
      481 tgtgtgcagg ggctgatagt caaggcgagg cttacatacg cgctttgcta aataataaaa
      541 aagatgagca aagtcgtaat gcgttattat ccatgttttc tgtcttttca attagccagc
      601 aacaaattaa taattttgat tttgaatttg aaatgttatt gcccgatgac gatgaatctt
      661 tagtcactcg tgcccaggct tttagtgagt ggtgtgaagg atttacgcaa gggttaacta
      721 ttgcaggagt tggaatggag cagttttatg aggaggaatc tcaggatgct cttcagcacc
      781 ttatagaatt tgcagagctg gattgtgagt cgttggaggt aggcgaggaa gatgagagag
      841 cgttgatgga ggtcagtgaa tacactcgta tggcagtact tcgattacac agcgatttgg
      901 ttttgcacga aagagaactt ggagattctg gaacaaccca ttgatagcaa gttgataaag
      961 tatcaaagtt gtttgtattt attggtttct ttgattttta aggattgctc atttgttcta
     1021 tttattaaag tgaaaaggga aattgatatg ataagccaac aagaatataa ggcgagaaga
     1081 aaaaaattag ctgcgcaatt gcctgatgac agtgttgctg taatacctgc agcacatgaa
     1141 gtgctgcgca gtggagatgc tcattatcga tttcgacagg acagtgattt ttattattta
     1201 acaggattta atgagcccga tgctttgctg gtcattattg ctggaaagga tagccaaagt
     1261 attttgttta atcgtccaag aaatcctgca gaagaacaat ggacaggtaa acgattaggt
     1321 caggacggtg ctttgcaaga gcttggagtg caggcggcat acccattaag cagtattgca
     1381 gaagagcttc ctaaactatt cagcgggaaa tctgccatat actatgacct cggccgtaat
     1441 ttatcattgg aaaaaaaaat caggcatgtt ttatcttcac taaaagctca ggtgagaaga
     1501 ggcattaagg cgcctgagtc gttatgcgat ctcgaaccta ttctaagcga aatgcgtttg
     1561 cttaaaagcg atgcggaatt agagttaatg agacaggcag cccgtatttc agtagcggct
     1621 cataaacgag cgatgcaagt ttgtcagaaa atggaatatg agtaccaact ggaggcagaa
     1681 ttaatctatg agtttagccg tcatggctgc cgtagtgtag cttatgaccc tattgtgggt
     1741 ggtggcgcca atgcctgcat tcttcattat acagaaaata acaaaccttt gcgccgtggc
     1801 gatttggtgc tcattgatgc agggggcgaa tatggtagtt atgccgcaga tattaccaga
     1861 actttcccgg taagtggtac ttttagccct gagcagaaaa tcatttatga attggtattg
     1921 aaagcacaaa aggctggcat agcagcgatt gaaccgggcc ttccctggaa tcatattcag
     1981 cagactatta tcagaattct tactgagggt ctctgtgata taggaatttt aaagggcgat
     2041 ttggaggagc tcattaaaaa tgaagcctat aaaccatttt atatgcataa ttcaggacat
     2101 tggttaggat tggatgtgca tgatgtcggc cgatataaaa tcaataatga atggcgtcct
     2161 ttagaggccg ggatggtatt aacagtagaa ccagggctgt atatcagtgc agggatggag
     2221 ggcgttgata aacgatggtg ggatatcggt gtgcgtatag aagatgatat tctggtaacc
     2281 agggcgggat atgaggtatt gaccggtgac ttgcctgtta atgtagatga tattgaggct
     2341 ttgatgcgtg gcgaataaag agactgatat actgattatt ggcggtggtt tgactggtgc
     2401 aagcttatcc ctggctctgc agggattagg tttctctgtc acgttagtag aatccaggcc
     2461 atacagtgat aaggtaaatc ctgactttga tgctcgatca ttggcgttgt cacctgcaag
     2521 tcaacgtata ttggcaatgc tgggagtttg ggagattctc aaagaatata caacaccaat
     2581 tcatatgatt catgtttctg atcagcatcg ctttggggtt tctcgtttgc atggcgaaaa
     2641 aaatagtcct ttgggctatg tggtagaaat gcaatacatc aacagggctt tgagtattct
     2701 aatcaataaa gaacacttgt tagctccagc ggctttaaaa tccatcgatt ttaacaattc
     2761 tactgccact gttgctcatg gcgacgatat gatttctatc caggctcaat taattgtcgc
     2821 ggccgatggg acggaatcga tgactcgcca cctctgtaat ctgccagtca aaattaaaga
     2881 ttatgctcaa gacgctattg tcgccaatgt gggcctgata aaacctcatg gtaatcgtgc
     2941 ttttgagaga tttacgccta atggtcctct tgcattattg cctatgatga atgacaaaat
     3001 gtcattggta tgggcatcaa agcctgaaga agcaaaacgg ttagtgatgt tgaatgatgg
     3061 tgattttttg cgagaactgc aaactgcttt tgggtatagg ttaggacgat ttggtaaagt
     3121 aggaaagcga tactcttatc ccctgaaaca agttttaatg ccccagcaaa ccaaatggcc
     3181 cgttgttttt gttggtaatg ccgctcacac tttgcatcca gttgcgggtc aagggtttaa
     3241 cctgggttta agagatattg cggctttggc acaatgcatc agccaaaagg gattaaatcc
     3301 acagatgtta gaacattatt tgaaattacg agcacatgat cagcaaatga ttacctggct
     3361 aacagacggt ttgatacatc tatttacgag tcgtttaccc ggtatgggag tagcgcgtaa
     3421 ccttggattg ctggcacttg ataatatacc ggcattaaaa aatttattag cacgatatgc
     3481 gcgtggtttt ggcggaatta ctcctgattt ggtttgtgaa attgcattaa gcactggaga
     3541 atttcaatga gccaacattt tgatgtactt gtcattggag gcgggattgt tggcttgaca
     3601 gcagctttag ccatggccca acgccattat actgttgctg ttatcgatgc agggacattg
     3661 atggtcaata cagacaagag tgatttgcgt gtttatgcca tcaataaggc atcaaaaatg
     3721 ttgttaagtg aattgggagt gtggcagcac ctcgatgatg ctcgggtcgc tccttataat
     3781 aaaatgcacg tctgggatgg tgtgagtggc gcatatattg actttgattc tcgcagtatt
     3841 gctgagccaa atttaggggc aattattgaa gaatcaatct taaagcaagc tctgttgcgt
     3901 cgaatctcat cagagtccaa aataaattta tttcctcaaa gtgtaataga tgaggtgtgc
     3961 catttagagc ggagcatcaa gatctccggc caaaatactg cctgggaagg gcagttattg
     4021 atgatagctg acggagctaa ctctcctatg cgtcataagc ttaaagtgga tgtaaccagc
     4081 tggtcttatg agcagcaagc cttggtcgca acggtaaata gtgagaaatc acatcagaat
     4141 actgcttttc aagtgtttca tcctgatggc cctttggctt ttttgccatt atctaatctc
     4201 aatcaatgtt ctatcgtttg gtcaactcat gccgataatg cgatgcgatt gaagttactt
     4261 gatgataatg aatttagtcg ggaattgact agggcatttg gaggtcagct agggcaaata
     4321 aaggtagaaa gcgatcgcca tcaatttcct ttacaaatga gacatgtcaa gcaatatgca
     4381 gggcatcgct ggttattgct tggtgatgca gcccacacta ttcatccatt agccggtctt
     4441 gggttaaatg ttggcctggc tgatgtgaaa agctggttaa cctgtcttga aatgggtaaa
     4501 ggtgaattga tatccaaaaa agtattaggg gcataccaaa gggaacgcaa gcattctgtt
     4561 tggcaaatta ttttgcttat ggagggattt aaaagattat ttggaacctc cgcatctcct
     4621 gttacctcat tacgcagtct cggattgcgg gcatgcaatg aatttatccc aattaaacga
     4681 ttatttatgc gccaggctag tggtttataa gtaattttcc gccaaagtaa ggcttttttt
     4741 caaaaaagat ataatgctta atttttagtt aataattgtt atgtataatt tttttgctaa
     4801 cctaaaaaac tctccgaggt tttaaatcac ttactcttat ttctgaagtt ttttaggttg
     4861 cctactattc tttagaggtt tggtaatgac cagattaaaa tttttttcca agtttcaaaa
     4921 agcattactt cagttggaaa ccaatgtctc aaatttgagt aatcctctgg tgggctcgaa
     4981 aatcaaggaa ttacagacta aaaaaatatt ggttcgagca gacggacgta gcttagatca
     5041 tttacacaaa ttaggtggtt tgccacaacg tgttgaaagc gagaagctgg aaaaagttcg
     5101 tatcaccgat gtggaaagat accagaaatt taatatgaat ccatttggat ggggcgcttg
     5161 tgcctctgca gaggacttgc gaaaattcct ggagaattat tccgaattga ctaaacaagc
     5221 atgggttcat aaattttatg gaagttcaac ttctcttctt accttaaagt ctgaagtagg
     5281 aatatcatgt ggtgatcatg atgaggaaaa agaagaactg gttgtcgata gcgtatcatt
     5341 tgaacaaatt attgcatcta cctgcccaaa atatagggaa aaataccttg gtggaggatt
     5401 ggtccccaat gctatgaagg attttaagtc caaagtccca catactatga gactaggtga
     5461 tttcagctcg gaaaaaagca ctttagaatg gttattgatt aatgattgtg aagaagagtt
     5521 tgccaagctt tgcaaagaaa tatacggaga tacccctctt aaaattttgt tgatgcgatt
     5581 tgagggggat aaatatcttg cagatgctgt tacttattat gtgaaagaag ctgcatcatc
     5641 ccctagggtt taatgctcga cgagagtcgt ggcatggcat tggctgacaa ttcaacgcga
     5701 tgatccttat ttatttgtac ttcaacatgg tgagatggat ctaaaataag aggattcata
     5761 tgatgaactt ggtcaaagcg gatcgaacta ttatttccaa tcatggattc aacgctacca
     5821 tatccccact catttcctgt gagggcaaac gcatctgaag gattaatcgc agcagggaag
     5881 tatttttcaa tctcgtcttc tcgaaactcc aaaacatcgc gtgtgctttg atacactttg
     5941 gtcgagccat tatagtcttc aaaaattgaa tcaaactgtt cttgttgaat ttcattaaaa
     6001 ataggaaatt caatttttgc tatccatgga aatttatgtt cacttaacag ttttttatag
     6061 gctcgtaaat aataaggaaa aatcatatgt tgtatatcat aaccgcaatc aatttttgca
     6121 aaaaaaccca tcccaactgc tgtggcttta aggaaggctg cttttccagt ttcttttgca
     6181 gtttgattta ctgacgccag cagtaatgaa acatcttcta gctgatagcg aaaataggct
     6241 tcagataaga aaataatggc gtttgacttg ctaaagctgg gataaaaacc acctgatgaa
     6301 aaccttgcca gataaggaaa accaaggcga atgcgagcca tcacgttgtt ggtatcatac
     6361 ctacctaact cctttttcgc atcatcaaag tgaattggta aaccatcatg caaatggcta
     6421 aatagcggtg aatcaatt
//
LOCUS       DAXLDT010000165         6372 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXLDT010000165 DAXLDT010000000
VERSION     DAXLDT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.165"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            340..792
                     /locus_tag="ACF2SP_002614"
     CDS             340..792
                     /locus_tag="ACF2SP_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360385.1"
                     /translation="MNRKSHYNNTFRDELRKKIDHITSRVFSRGDLIQDHSNQAQLRL
                     NRALRTFMDEGIIIKIAHGLFAKAEPMSIPGRKMQAVLTEPFETVATEALDKLGIKWE
                     LGSAIRDYNAGKTTQVPAVFSVRLKSRYRGSIHAEGRKLIFEGGINAR"
     gene            782..1708
                     /locus_tag="ACF2SP_002615"
     CDS             782..1708
                     /locus_tag="ACF2SP_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFD2360386.1"
                     /translation="MPGELNEQQIRQTAIILGLPADFIRKDYFVTKAIRLLVGISNDY
                     FELVFQGGTSLSKGYGVISRLSEDIDFRVISKPACLNLGKDARRRELRLFRRALVESL
                     INAGFYIQPQDVKVFYEGRYMSIRASFDGAQSIVYLKPHIAIDCFLGTLELEPEIRNI
                     SSMVKVTLGDTECDHQYFPVPCVALDETAAEKWVALSRRIVDANKTVRDSDKHLVRHL
                     YDLHQLQQQNCLKGNYYLVLEKVLEKEQEMFGGESSVSVYEKSISALHYLRSDKKWEE
                     HWQFFLEQMVYQLEKPSFEQAIATVEKLNSRR"
     gene            complement(1730..2236)
                     /locus_tag="ACF2SP_002616"
     CDS             complement(1730..2236)
                     /locus_tag="ACF2SP_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFD2360387.1"
                     /translation="MDTPQIYIACLAAYNNGILHGEWIDATQDVSTIYNEIHEMLANS
                     PIEDAEEFAIHDFEGFGGTQIGEYDSINDVAELAFFIMKHGELGAELFSYYSIEDAER
                     MLEENYHGAYDSEVDFARALFDDYYNNAIPEDLLFYFDYEAFARDLFINDYFSVEADG
                     QTHVFSSY"
     gene            complement(2293..2484)
                     /locus_tag="ACF2SP_002617"
     CDS             complement(2293..2484)
                     /locus_tag="ACF2SP_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360388.1"
                     /translation="MENVSSIISRKEYAFFDLACCINEFHTLRYELSAQDIEEIKRLS
                     KKLHSLLEHQHPHFNLVKP"
     gene            complement(3139..4200)
                     /locus_tag="ACF2SP_002618"
     CDS             complement(3139..4200)
                     /locus_tag="ACF2SP_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFD2360389.1"
                     /translation="MMAAKLNFSSSIVIALLAAILFGASTPFAKQLLGGISPVLLAGL
                     LYLGSGIGLLLFRLIKDKGWQASRLFNNEWIWLIGAIGFGGILGPVLLMLGLEHTSAA
                     TASLLLNLEAVLTALLAWVFFKESTDRRIIFGMLLIVAGGILLAWPNQLGQQHWAGTI
                     TIALACLCWAIDNNLTRRISTADALFIASSKGLVAGMTNVSLAFILHLPLPGWTQVSY
                     ALLIGFFGYGISLVLFVLALRDLGTARTGAYFSTAPFIGAAIAILLFGSTTSILFWIA
                     AILIAMGVWIHLTENHEHIHTHEYLFHHHSHVHDEHHQHNHNFFGDGKEPHAHSHEHK
                     LMTHSHHHYPDIHHEHKHK"
     gene            complement(4372..5274)
                     /locus_tag="ACF2SP_002619"
     CDS             complement(4372..5274)
                     /locus_tag="ACF2SP_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HFD2360390.1"
                     /translation="MSNTKLRSAKFSINELVKKAYGHSYASQSDMRHMLFRCVKDLHE
                     LGYKIGHIKGLKAKHIYVLVDHWKSEGKNPGTIKNHMAKLRKAACLLDNPKLIKPENS
                     AYNISQRSYVSSINKAIHHIDLNKCTDPYIRLSLEGQMLFGLRREESMKFTLSEAHHG
                     DSLIIKPSWTKGGIGRTLNITNEAQRQWLTKVSQLVRKGESLIPSNRTYKQHLSQYEE
                     QAKAMGVSKLHGLRHAYAQRRYRELTKLFDPQSQGMPCPFEGGKKFNNMNPIEKAMDR
                     KARQMISLELGHSRLSITRIYCGK"
     gene            complement(5267..6070)
                     /locus_tag="ACF2SP_002620"
     CDS             complement(5267..6070)
                     /locus_tag="ACF2SP_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HFD2360391.1"
                     /translation="MRKSQLRQQVDQYCKHNHTGSFRTKKHRHFVLYKMIRDLYHIGH
                     VPPKWHAVTHDHMVQLVVHWQKEGIKPKTIMKYMTLIRRFFQSIDHTIDNISNQHLGI
                     KSVQIRSKTTVFPNNYSEILTNPIAKIVLEFQIYFGLTLSEAMRLNPAIHIQENSLWI
                     TRDIATNSHDRRIPVRNDRQAQANTVLQTLCKEESLILTFGYHSVRDAYSHAIKKIGL
                     SSSKSYRCFYAKSMYPELCQILSPYLAKQTIMREMGLQSRRTLWSYLHE"
BASE COUNT         1951 a         1163 c         1221 g         2037 t
ORIGIN      
        1 tctacattgc gtctacatga ctttttcgaa ctttctgaaa actactgtaa ctacttgatt
       61 ttattggtgg gcccactagg acttgaacct aggaccaacg gattatgagt catcaatctc
      121 tattttaact acttgatttt attataaata ttgctgcgta acaaccacta caaatggcgt
      181 actatggcgt acagtgtcgt actcaaacac gcaaatctca cgcgaggcta ttttagcttc
      241 ttgtgaggta aattatcaat tatttattaa aatgactatt tgtgacgtaa tattgtgcta
      301 taataaaaat gactatttat gacgttatgg taatcagcta tgaaccggaa aagtcattac
      361 aacaatactt ttcgtgatga attaagaaaa aagattgatc atatcaccag ccgtgtattt
      421 tcgcgggggg atttaataca agatcattcc aatcaagctc aattaagatt aaatcgagca
      481 ctgcgtactt ttatggacga gggtattatc attaaaattg cacacggttt atttgctaaa
      541 gctgagccta tgagtatccc tggtagaaag atgcaagctg tactaacaga gccatttgaa
      601 acagtcgcaa cagaggcgct agataaatta ggaattaaat gggaattagg aagtgctatc
      661 cgcgattata atgcggggaa aactacccag gtgccagctg tttttagtgt gcgattaaaa
      721 agtcgatatc gtggttctat tcatgcagag ggtagaaaat tgatttttga aggtggtatc
      781 aatgccaggt gaattaaatg aacagcaaat tcgtcaaact gccattattt taggattgcc
      841 cgcagatttt attcgcaaag attatttcgt tacaaaagca attcgtttat tagttggaat
      901 atctaatgat tattttgagt tggtatttca agggggtact agtctttcta aaggttatgg
      961 agtgattagc agactctcgg aggatatcga tttccgagta atttctaagc ctgcttgtct
     1021 caatttaggt aaagatgcaa gacgacgaga gttgcgatta tttcggcgtg cattggttga
     1081 atcgttaatc aatgccggct tttatataca accacaggat gttaaggtct tttatgaagg
     1141 acgatatatg agtattcgtg cttcatttga tggtgcccaa agcattgtat atttaaagcc
     1201 ccatattgcc attgattgtt ttcttggtac gttggagtta gagcctgaaa ttcgtaatat
     1261 ttcctcaatg gttaaggtga ctttgggtga tacagaatgt gatcaccagt attttccagt
     1321 tccttgtgtt gcattagatg agacagcagc ggaaaaatgg gtggcattgt cacgtcggat
     1381 tgtagatgca aataaaaccg tgcgcgactc agataagcac ttagtcaggc atttgtatga
     1441 tcttcatcaa ttgcagcaac aaaattgcct taaaggaaat tattaccttg tattggaaaa
     1501 ggtgttagaa aaagaacagg agatgtttgg cggtgaatct tctgttagcg tatatgagaa
     1561 atcaatatca gcattacatt accttcgttc agataaaaag tgggaagagc attggcaatt
     1621 ttttctggaa caaatggttt atcagttaga aaaacctagt tttgagcaag ctatagcaac
     1681 ggtggaaaaa ttaaactcaa ggagataata aaagccccaa agaaggggct tagtagctag
     1741 aaaaaacatg cgtttgacca tctgcttcta ccgaaaaata gtcgtttata aatagatcac
     1801 gtgcaaaagc ttcataatca aaataaaaca gcaaatcctc cggtattgca ttgttgtagt
     1861 agtcgtcaaa aagcgcacgg gcaaaatcaa cttcactatc ataagcaccg tggtaatttt
     1921 cctctaacat cctctcagca tcctcaatag agtagtagct aaaaagttca gcacctaatt
     1981 ccccatgctt cataatgaag aaggcaagct cagcaacatc attgatgctg tcatactcgc
     2041 caatttgtgt accaccaaac ccttcaaaat catggatcgc aaactcttct gcgtcctcaa
     2101 tggggctgtt agccaacatt tcatgaattt cattataaat ggtacttaca tcctgagttg
     2161 catcaatcca ttctccgtgt aggatgccat tgttataagc tgcaagacat gcaatataaa
     2221 tttgaggtgt atccatttta agactccttg caaaagaagt gctattccct aagcagaaat
     2281 aagcactcct acttagggtt taactaaatt gaagtgagga tgttgatgct ctaaaagaga
     2341 atgcaacttc ttgcttagcc gtttaatttc ctctatatct tgtgctgata actcatagcg
     2401 caaggtatga aattcattaa tacaacaagc taaatcaaaa aatgcgtatt cctttcttga
     2461 gataatagaa ctcacatttt ccatggtttt ctccaaaatt gggaaaccat cccatcaggg
     2521 aagtctttcc cagatgatta aaaaaatgag tgatccatac ttgattacaa taagcctggt
     2581 tgattcgcct aatttttaaa gagcagccgt tcgcaaataa acaattgcga atggataaaa
     2641 aggtttaaaa caagaggttt taatgtggtt tcaccctggt gggtgatcag tgatcgaaac
     2701 tgaggttccc gacttaacgt tctgccataa gattagggaa taatagtggc tcttcacaag
     2761 accaattggg tttcgccaga ctctcactgg ctcttcagtt gtgttatact tagtaatcta
     2821 aactattatg acaaacaaat caatcaaaac gtgttttaaa tttaaaaata aaacacgttt
     2881 tgattgattt gttttcagcc tcaataaatc ttatgtgctg caacatataa aatttattgt
     2941 ggaaagtaag ttattatgtt cagtagccaa aaaaaacata aattttacat tgagttaatc
     3001 ggaaactact tcacataaaa tctctactct ttctgttaag cacttatagt tacctcagtg
     3061 agttaattgg gataattatg tgcaaattac acttaaatga atgagatgtc ttgtaattaa
     3121 ctatgaatag atagtcggtt atttatgttt atgctcgtga tgaatgtcag gataatgatg
     3181 gtgagagtgt gtcatcaatt tatgctcatg cgaatgagca tgaggctctt taccatcccc
     3241 aaaaaaatta tggttatgtt gatgatgctc atcatgaaca tggctgtgat gatggaatag
     3301 gtattcatgt gtatgtatat gttcatggtt ttccgtcaag tgtatccaaa ctcccatagc
     3361 tattaatata gccgctatcc aaaataggat agaggtcgta ctaccaaaaa gtaaaatggc
     3421 tattgctgct ccaatgaatg gagccgttga aaaataagcg cctgttctag ctgtccctaa
     3481 atcccgtaaa gctaatacga ataaaaccag gctgatacca taaccaaaaa aaccaatcag
     3541 taaagcataa cttacctgtg tccagcctgg taaaggcaag tgcaatataa aagcaagact
     3601 gacgttcgtc atacctgcaa ccaatccctt actgcttgca ataaacaatg catcggctgt
     3661 tgatattctt cttgttagat tattatcaat cgcccagcac aaacaagcta atgcaatagt
     3721 tatagttcca gcccaatgtt gttgtcctaa ttgattaggc caagctaaaa gaataccacc
     3781 agccacaata agcaacatgc cgaatataat ccgtctatct gtgctttctt taaaaaagac
     3841 ccaagctaat aatgctgtca acacggcttc caaattaagt agcaatgaag ctgttgctgc
     3901 actagtgtgt tctaacccca acatcaataa aactggccct agtatgcctc caaatccaat
     3961 agcacctatc agccaaatcc attcattatt gaatagccta gaggcttgcc aacctttgtc
     4021 tttgattaat cgaaagagca gcaaacctat accacttcct agatataaca atccagctaa
     4081 taagacaggt gagatgcccc ctagcagttg cttagcaaaa ggggtgcttg caccaaataa
     4141 aatagcggcc aaaagcgcta ttactataga agaagagaaa tttagcttag cagccatcat
     4201 aaccacactt taaataatat taagttcatt aatactgttg aattcaaaaa acaccaaatc
     4261 tgcgccaata tatacttata agcttagtgg aaattcaaga tattatgtgc agtgatttta
     4321 atcatgaacc ttgaaaaata attaaaactt ttctctattg cacataacat tctactttcc
     4381 acaatagatc cttgtgattg acaggcgaga gtgtcctaat tcgaggctaa tcatttgacg
     4441 tgcttttcta tccatggctt tttctattgg attcatgttg ttaaattttt ttccaccttc
     4501 aaatggacat ggcattccct ggctttgtgg atcaaataat ttagtgagct ctcgatatcg
     4561 tctctgagca taggcatgtc ttaagccatg caatttacta acccccatgg cttttgcttg
     4621 ttcttcatac tgacttaagt gctgcttata ggttctattt gatggaatga gcgattctcc
     4681 tttccttacc aattgagaaa ccttcgttaa ccattgacgt tgtgcttcat ttgtgatgtt
     4741 taatgttcgg cctatacctc cttttgtcca actgggttta atgatcaagc tatctccatg
     4801 gtgtgcttcg cttagtgtga atttcatgga ttcttcacgc cttaagccaa agagcatttg
     4861 gccttcaagg gataatctaa tgtaaggatc ggtgcactta tttaaatcaa tatgatgaat
     4921 cgccttgttg attgaggata cataagagcg ttggctgatg ttgtatgcac tattttctgg
     4981 cttaataagt ttgggattat ccaacagaca ggctgctttg cgtagtttgg ccatgtggtt
     5041 tttaattgtt cctgggtttt ttccttcaga tttccagtga tctacgagta catagatatg
     5101 ttttgctttt aatcctttta tatggccaat tttatagcca agctcatgta aatctttcac
     5161 acatcgaaag agcatatgac gcatgtctga ttggctggca taggaatggc catatgcttt
     5221 tttaaccaat tcattgattg aaaattttgc cgaacgcaat tttgtattac tcatgcagat
     5281 aactccaaag cgtgcgtctg gattgcagcc ccatttcacg catgattgtt tgtttcgcta
     5341 ggtaagggga caagatttga cacagttctg gatacatact cttggcataa aaacagcggt
     5401 agctctttga tgatgacagg cctatttttt ttattgcatg gctgtacgcg tcgcgtacag
     5461 aatgatatcc aaaagttaaa atcaaacttt cttctttgca tagtgtttga agtacggtat
     5521 tggcttgagc ttgcctatca tttctaacag gaatccttct gtcatggctg tttgtggcaa
     5581 tatctcttgt aatccaaagt gaattttcct gaatatggat agcaggattt agacgcattg
     5641 cttcactaag cgttaatcca aaataaattt gaaattcgag cactattttt gcaataggat
     5701 ttgttagtat ttccgaataa ttatttggaa atacagttgt cttggagcga atttgaacgc
     5761 ttttaattcc caaatgctgg ttactaatat tatcaattgt atggtcaata ctttgaaaaa
     5821 atctacgaat gagggtcatg tatttcatga tggttttggg tttgatcccc tctttttgcc
     5881 aatgaacaac aagttgcacc atatgatcat gagtaactgc atgccatttg gggggaacat
     5941 ggccaatatg atataaatca cgaatcattt tgtataatac aaaatgacgg tgttttttcg
     6001 tgcggaatga ccctgtgtga ttgtgtttac aatactgatc tacttgttgt ctgagctgac
     6061 tttttctcat ctgtaaccct ctagaacttt ttgccctggt attttgttta gtgttggtag
     6121 agcggcctgc aagattgcag taccaggcgt agtgcctcaa ggctcagttc aggggcaaat
     6181 cagttttaaa ctaaaacttc tattgacctg attacatagg tactactttt ttatggtggt
     6241 gatctcctta ggtttaatgg ttttaaatac aacaaagtct ctacgaacta ttgttgcgca
     6301 cgggcaatac agagagggta ttgtgaggtt caattcttaa agaattgtat tgcttttcat
     6361 tacaatagat tc
//
LOCUS       DAXLDT010000166         6311 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXLDT010000166 DAXLDT010000000
VERSION     DAXLDT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.166"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            202..966
                     /locus_tag="ACF2SP_002621"
     CDS             202..966
                     /locus_tag="ACF2SP_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFD2360392.1"
                     /translation="MKDIIIASFYKFIPLNDFESLREPILTKMHEIGIKGTIILAHEG
                     VNGGFAGNREQMNVFYDYLRSDSRFADLHFKETYDNKNPFDKAKVKLRKEIVTMGVQK
                     VDPSYNTGTYLSPEEWHQFIQDPNVILLDTRNDYEYELGTFKNAINPDIENFREFPDY
                     VQRNLIDKKDKKIAMFCTGGIRCEKTTAYMKEQGFQHVYQLHDGILNYLESIPESESL
                     WEGKCFVFDDRVAVDQKLDRVYPQLPQDYKYEREQK"
     gene            1078..1548
                     /gene="smpB"
                     /locus_tag="ACF2SP_002622"
     CDS             1078..1548
                     /gene="smpB"
                     /locus_tag="ACF2SP_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFD2360393.1"
                     /translation="MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAG
                     KINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPDPVRTRKLLMNKKELNHLIGSVE
                     RQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT"
     gene            1560..2024
                     /locus_tag="ACF2SP_002623"
     CDS             1560..2024
                     /locus_tag="ACF2SP_002623"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFD2360394.1"
                     /translation="MKIGEMVPDFAFNATNNVQGHLNDYKGQIIVLYFYPKDATPGCT
                     TEGQDFRDAYSQFKDLNTVIFGISRDSLKSHEQFKSKQNFPFELISDDNETLCQTFDV
                     IKMKSMYGKQVRGIERSTFIIDTQGVLTHEWRKVNVKGHVEEVLKAVRALAG"
     gene            2148..3347
                     /locus_tag="ACF2SP_002624"
     CDS             2148..3347
                     /locus_tag="ACF2SP_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360395.1"
                     /translation="MFIRNLQLFIVVASCILVLIGTDILLPSLPNIAHSLSVSSNEAK
                     MLISIFMIGQFSTVLIWGVIADRLGRRKTLFLGMLIFFIGSIFSLKADSIQLLLACRF
                     LQGTGAVVVPVAGWALVQDLFPKDEGARIMTWIGTLTAVIPLFAPALGGQLDVLYGWR
                     SNLYCIAFYSFILCVIMLFLPRTIPVQKTTTADIKETLSIYIRIIKNKTFISYIALFG
                     LLNCGEWCFLTVAPFYYSHAHIAADTMGILLMITSMGFVCGSLLASRLFNRLGIDKII
                     ALGIQLAVMSSLMLLIGEYFHWSKYQLYNAITIGVYILSSALLWGATTSRALQCFDDC
                     RGSASAIRSLILLCFAAFGTYSGRLVNHNSLFPVGLFLLFMAFIAFIVFNNKELKTER
                     HIEGIPI"
     gene            3502..4908
                     /locus_tag="ACF2SP_002625"
     CDS             3502..4908
                     /locus_tag="ACF2SP_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFD2360396.1"
                     /translation="MDNTNTGLKLFVLDTNILMHDPTAIYHFDEHDIYLPMVVLEELD
                     SHKTGTSELARNVRQTNRMLVELMSNATHDQIVSGLPIPSYINSSNKKCSGRLFFQTD
                     EFDQVVPSTLPGHKVDNTILATALGLQKKYYGKRQVIIISKDINLRIKAGILGIHAED
                     YYNDQVLDDVNLLHRGLHILDNNFWDSHAKDMGSWQESGKTFYKVTGPLVSQWNPNDC
                     LSTEDDQFQALVKKIDGPNAIVQLIRDYTQPKHSVWGITARNREQNFSLNLLLDPDID
                     FVSLQGSAGTGKTLLTIAAGLTQVMDQNRYSEILMTRVTIPVGEDIGFLPGTEEEKMT
                     PWMGALMDNLEVLHSSQVGGSFGRGATQDLLQNKIKIRSLNFMRGRTFLNRYIIIDEA
                     QNLTSKQIKTLVTRAGPGSKIICLGDIKQIDTPYLTETTSGLTFAVDRFKSWEHSAHM
                     TLTRGERSRLAFYAAEHL"
     gene            4931..5944
                     /locus_tag="ACF2SP_002626"
     CDS             4931..5944
                     /locus_tag="ACF2SP_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HFD2360397.1"
                     /translation="MTDQAITFDDVLLVPSYNHHESRRVVETTSTDRLGKLTLNLPVI
                     SANMDTITESNMANFMHSKGAMGALHRFMTIEENIQEFKKCKGPVFVSVGCTENELQR
                     AEALRDAGADFFCVDVAHAHAKYVGKTLKSLRQLLGSRCIMAGNVATYAGADYLASCG
                     ADIIKAGIGGGSVCSTRIKTGFGVPMLTCIQDCSRADRSIVADGGIKTSGDIVKALAF
                     GADFVMIGGMLAGSAPTPGEVIQKDDGSKVKRYRGMASREAQEAFLGQMHEWKTAEGV
                     ATEVPFKENPDGIIADIIGGLRSGLTYAGADSISELQRKLNYVIVTQAGRIESLPHKL
                     LEG"
     gene            complement(5978..>6311)
                     /locus_tag="ACF2SP_002627"
     CDS             complement(5978..>6311)
                     /locus_tag="ACF2SP_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg2844 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360398.1"
                     /translation="SQSSSPPTDSSSSGSDSQSSSPPTDSSSSGSDSQSSSPPTDSSN
                     SSSSSSSDTSSQLSPPPDSNTNTSTPDLGSQTKYDETSSSSNSGSGQSSGSGSSSDVP
                     TPNQSMPG"
BASE COUNT         1911 a         1240 c         1362 g         1798 t
ORIGIN      
        1 gctcctcgca acgacggtcc caaaaatcgt accgtacaga gatacttaag tactaacatg
       61 caataacgca actatttttc acccaatatg ccgttaaaat cccgatgtcc aggaaatgca
      121 tttaattcat tcctattgat taaattgtga gtaaatgata atttttgcga attgattttt
      181 tgttaatcct taggttacag cgtgaaagac attattattg catcatttta taagttcatt
      241 cctctcaatg attttgaatc actgagagag cccatactca ctaaaatgca tgaaataggc
      301 ataaaaggaa ctatcatcct ggcccatgag ggcgtcaacg gcggatttgc cgggaataga
      361 gaacaaatga atgtttttta tgattacctg cgttctgatt cgcgctttgc cgatctgcat
      421 tttaaagaaa cctatgacaa caaaaaccct tttgataaag cgaaagtgaa actgcgtaaa
      481 gagatagtaa ccatgggcgt ccagaaagtt gacccctctt ataatacagg gacctatcta
      541 agccctgagg aatggcatca attcattcaa gatcctaatg ttattttatt agacacccgc
      601 aatgattatg aatacgaact gggtactttc aagaatgcaa ttaatcctga catagaaaat
      661 ttcagggaat ttcccgacta cgttcagcgc aatttaattg ataaaaagga caagaaaata
      721 gccatgtttt gtactggcgg aattcgttgc gaaaaaacga cagcttatat gaaagagcag
      781 ggctttcagc atgtttacca acttcatgac ggcattctaa attacctgga atctattcct
      841 gaaagcgaat cactttggga aggcaaatgt tttgtttttg atgaccgcgt tgcagtggat
      901 caaaaattag atcgtgttta tccacagtta cctcaggatt ataaatacga aagagagcag
      961 aaataacgta gaatatactt ttttgccata agccgctcaa ggcaacaaaa ttaagaaaag
     1021 cagttcaatg ggatagtaca tcagttatac tgtttttttc aacaaatcag atgaattatg
     1081 actaccaaaa aacaaccaga ttccaccata gccttgaata gaaaagctgg ttttgattat
     1141 tttattgaag atcaatacga agcaggcctg gttttggagg gctgggaagt aaaaagtctg
     1201 cgtgctggga aaatcaattt gtcggattca cacgtgataa tcaaatacgg tgaggcattc
     1261 ctattgggcg cccaaataca gcctcttccc accgcatcca ctcattttat tcctgatccg
     1321 gtcaggacgc gcaagctatt gatgaataaa aaagaattaa accatctcat tggaagtgtt
     1381 gaaaggcaag gctataccat agtccctctt tctttgtatt ggaaaaaaaa taaaattaaa
     1441 ataaaaattg ctctggccaa aggaaaaaaa gagcatgaca aaagagacac gatcaaagac
     1501 agagaatggc aaagagatcg ttcaagaata atgaaaaaga acacttgagg agatggttta
     1561 tgaaaatagg tgagatggtc cccgattttg cttttaacgc cacgaacaac gtgcaaggac
     1621 atcttaatga ttacaaagga cagatcatcg tactttattt ttaccccaaa gacgcaaccc
     1681 cgggctgtac gactgaaggc caagacttca gagatgccta ctcacaattt aaagatttaa
     1741 atacggtgat tttcggtatt tcgagagata gtttaaaatc ccatgaacaa ttcaaaagca
     1801 aacagaattt cccttttgaa ttaatcagtg atgataatga aacattatgc caaacgtttg
     1861 atgtgatcaa aatgaaatcc atgtatggca aacaagtcag agggatagaa cgaagtactt
     1921 tcattataga tacacaaggt gtcttgacac acgaatggcg taaagtgaat gttaaagggc
     1981 acgtagagga agttctaaaa gcagtgcgag ctttggcggg ataaaaaaat aatccctgat
     2041 aaggactcct gttttgaaaa agtacttttt atagagtatt taattaattt ttgctattat
     2101 tgttgcggat aaatacatca aatacaccct tttcttaaga gttcaatatg tttatccgca
     2161 acctgcaact ttttatcgtt gtcgcctctt gcatactggt tttaattggc acagatattt
     2221 tgttacccag cctgcctaac attgctcact cactgtctgt ctcatcaaat gaagccaaaa
     2281 tgctcatttc cattttcatg ataggacaat tttctacagt tcttatttgg ggcgttattg
     2341 ccgaccggct aggcagaaga aaaacattat ttctggggat gcttattttc tttataggct
     2401 caatcttcag tttaaaagcc gattcaattc agttgttact agcctgtcga ttcttgcaag
     2461 gtactggcgc agtcgttgtc cctgttgcag gttgggcttt agtccaggat ctcttcccca
     2521 aagacgaagg agctcgcatc atgacctgga ttgggacact gactgcagtg attccactct
     2581 ttgcccccgc attagggggg caattggatg tgctatatgg ttggcgttca aatctttatt
     2641 gtatcgcatt ttactctttc attttatgcg tcattatgct gtttttaccc agaactatcc
     2701 ctgtgcaaaa aacgacgact gctgacatca aggaaacatt aagcatttac attcgaatta
     2761 taaaaaacaa aacatttatt tcctatatcg cattatttgg tctattaaat tgtggtgaat
     2821 ggtgcttcct gacagttgct ccattttatt attcgcatgc gcatattgct gctgacacca
     2881 tggggatatt attaatgatt acttccatgg gatttgtctg tggatcgtta ctggcctccc
     2941 gcttatttaa tcgcctgggg attgataaaa tcatagcatt ggggattcag ttggctgtaa
     3001 tgagcagcct gatgttgtta ataggagaat actttcactg gagcaagtac caactctata
     3061 atgccataac tatcggcgtc tatattctaa gttcagcttt gctatggggc gcaaccacat
     3121 ccagagccct gcaatgcttt gatgattgca gaggctcggc ttccgcgatt cgcagtttaa
     3181 tcttactgtg cttcgctgct tttggtactt attccggtcg cctggttaat cataatagcc
     3241 tgttccctgt tggattgttt ttacttttta tggccttcat tgcgttcatt gttttcaata
     3301 ataaggaatt gaaaacagaa cggcatatcg aaggcatacc catttaactt aaaagcaaat
     3361 acttaacaat ataattcttg acggaaccac catagctcag cacaatgaac atagaattca
     3421 gggatttcaa tgcgctcact gcccaggcag gaaactgatt ccacttgaat tgaagaatac
     3481 tttagctgac agggtgaacc tatggataat accaataccg gacttaagct gttcgtgctt
     3541 gatactaata ttttgatgca tgatcctact gctatctacc attttgatga gcatgatatt
     3601 tatttgccaa tggtggtgct tgaagaattg gatagtcata aaacagggac atccgaattg
     3661 gcgagaaacg tgaggcaaac gaaccgcatg ttggtagagc taatgagtaa cgccactcac
     3721 gatcaaattg tctcaggact gcctatccca tcttacatca atagcagcaa caagaaatgc
     3781 agtggacgat tgttttttca aacagatgaa tttgatcaag tggttccttc aactctgccg
     3841 ggtcataaag tagacaatac aatactggca accgctttag gcctgcaaaa aaaatattat
     3901 ggcaaaagac aagttatcat tatttcaaag gacatcaact tgcgtattaa agcagggata
     3961 ttaggcattc atgctgaaga ttactacaat gaccaagtgc tggatgacgt gaatttactt
     4021 cacagaggat tacatattct cgataacaat ttttgggata gtcatgccaa agatatggga
     4081 tcctggcaag aaagtggtaa aaccttttat aaggtaaccg gtcctttggt aagccaatgg
     4141 aatccaaacg attgccttag tacggaagat gaccagtttc aagctttggt caagaaaatt
     4201 gatgggccta atgccatagt tcaactcatt cgtgactaca ctcaacccaa acattcagtc
     4261 tggggaatca cggcaagaaa ccgtgaacaa aatttttccc taaacttgtt actggatccc
     4321 gatattgatt ttgtcagctt gcaaggctca gcaggtacag ggaaaacact attaaccata
     4381 gccgcaggat taacccaggt catggatcaa aatcgctaca gcgagatatt aatgacaagg
     4441 gtaacgatcc ctgttggcga ggatatagga ttcttgccgg gtaccgagga ggaaaaaatg
     4501 actccgtgga tgggcgcttt aatggataat cttgaagtgc ttcacagctc acaagtcggc
     4561 ggcagttttg gacgtggggc aactcaggat ttgctgcaaa ataaaattaa aatacgctcg
     4621 ttaaatttta tgcggggaag aactttttta aaccgctata ttataattga tgaagcgcaa
     4681 aatctgacat caaaacaaat taaaaccttg gttacaagag caggcccagg tagtaaaatt
     4741 atctgtctgg gggacattaa gcaaattgat accccctatt taactgaaac cacttccgga
     4801 ctgacttttg ccgtagaccg gtttaaaagt tgggaacaca gcgctcatat gactttgact
     4861 cggggagagc gctcaaggct ggctttttat gcagctgagc atttatagta tttgtttatg
     4921 aggattaata atgactgatc aagcgatcac ttttgatgat gttctgctcg ttccatcgta
     4981 caaccatcat gaatccaggc gagttgttga aacaaccagt accgacaggt taggtaaact
     5041 gactttaaat cttccggtaa tcagcgccaa tatggatacg attaccgaaa gtaatatggc
     5101 taattttatg cacagtaaag gagccatggg cgctttacat cgctttatga ccatagaaga
     5161 aaatattcag gaatttaaga aatgcaaagg acctgtcttt gtatcagttg ggtgcactga
     5221 aaatgaatta caaagagcag aagcactgcg ggatgcagga gccgattttt tttgtgttga
     5281 tgtggcacat gcccacgcca aatacgtcgg caaaacatta aaaagcttgc gtcagttatt
     5341 aggaagccgc tgcattatgg ctgggaatgt cgccacttat gccggggcag attatcttgc
     5401 atcttgtggc gctgatatta ttaaagcagg tattggagga ggctcagtat gcagcacccg
     5461 aattaaaacc ggctttggcg tacctatgtt gacctgtatt caggattgtt cacgtgccga
     5521 tcgttccatc gttgctgatg gcggcatcaa aacatcgggt gatatcgtca aagcattggc
     5581 ctttggcgct gattttgtga tgataggcgg catgttagct ggctctgccc ccacgccagg
     5641 agaggtcatt caaaaagatg acggaagcaa agtcaaacgc tatcgaggca tggcatcccg
     5701 agaagcgcag gaagcattcc ttggacaaat gcatgaatgg aaaacagcag aaggtgttgc
     5761 aaccgaagtg ccatttaaag aaaaccctga tgggattatt gctgatatca taggcggatt
     5821 gcgctcaggt ctaacctatg ccggcgccga ctccatcagc gaattgcaaa gaaaattgaa
     5881 ttatgtgatt gtcacccagg ctgggcgcat agaaagcttg cctcacaagt tgttggaggg
     5941 ataactcacc accaacccgg acaatctatt ttcacatcta tccaggcata gactgattag
     6001 gtgttgggac atcagaagat gagcctgagc ctgaagattg accacttccg ctgttagaac
     6061 tgctggatgt ttcgtcatac tttgtttggc taccaagatc tggcgtactt gtattagtat
     6121 tcgagtctgg tggggggctc agttgtgatg aagtatcact actgctgcta ctgctactgt
     6181 tactactgtc tgtaggcggt gatgaagact ggctgtcgct gccactgctg ctactgtctg
     6241 taggcggtga tgaggattgg ctgtcgctgc cactgctgct actgtctgta ggcggtgatg
     6301 aagactggct g
//
LOCUS       DAXLDT010000167         6198 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXLDT010000167 DAXLDT010000000
VERSION     DAXLDT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.167"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            361..633
                     /locus_tag="ACF2SP_002628"
     CDS             361..633
                     /locus_tag="ACF2SP_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360399.1"
                     /translation="MNKKLSRLQLLNEFESAPTSALFNQQTLAAVLDCSSQLLERNRW
                     EGKGIPYLKIGRKVLYRKSDVLSFLQQQKIYRSTCDEGQVLSVANQ"
     gene            complement(734..1543)
                     /locus_tag="ACF2SP_002629"
     CDS             complement(734..1543)
                     /locus_tag="ACF2SP_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFD2360400.1"
                     /translation="MRTQSLRQFANRYIKLDRQGKYLYRKHRAYVIRKMIDDLFVIRQ
                     VPPSWQALQSEQIHKLVRYWKKQNINPVTIMRYMTIIRRFLQMNDCSVADIDNQSLEL
                     SRPKTRKKRKKTIPPDIWKSLHDPIARVIMGLQTEFGLTFKEAILIKPHIQVREGSLW
                     ITRDIAFNSTDRSIPIRTESQKAVLNLFNWLTKQYGNLLQLKSYEEIRIIWRSALAKH
                     CLSSSKSWRYLYAKQMYAYLLPNYGNYKICWLIRDEMGIKSRNTLWLYLKN"
     gene            2002..2148
                     /locus_tag="ACF2SP_002630"
     CDS             2002..2148
                     /locus_tag="ACF2SP_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360401.1"
                     /translation="MSYLIWCVRTNGVLRLGVGGVVMANIPVSLLENCSDAEKHSNEL
                     RTCC"
     gene            2246..3307
                     /locus_tag="ACF2SP_002631"
     CDS             2246..3307
                     /locus_tag="ACF2SP_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFD2360402.1"
                     /translation="MEYARHRPEQTLLYQVIEEYYPAFLSHLAELEKSVPQYVQDEFE
                     SYLKCERLEHGFLRVQCESCHVEQLVAFSCKRRGFCPSCGAKRMVESAALLVDEVLPE
                     KPIRQWVLSVPYPLRWLFASEPEMMSKALLIVTRAISSYLIKKTGFTHKTAKTGAVTL
                     IQRFGSALNLNIHFHMLFLDGVYGVDSGGAIGAFHGIKPPSSKEMSLLLGKISERIAR
                     LVEQAGYLERDEGDGLKLEGFEDEIMNHFQGSSITYKIAVGSQKGRKVLTIKTLPCKG
                     SKMSWAMRLKRVFNIDITVCRHCQGRVRIIACIEEQVVIDQILAHMNQQQQSRILVQV
                     SSWIRTPPIEQSSQMTMNY"
     gene            complement(3654..6020)
                     /locus_tag="ACF2SP_002632"
     CDS             complement(3654..6020)
                     /locus_tag="ACF2SP_002632"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2360403.1"
                     /translation="MLNKTDNTDLLPTIFNQDFLVDLFAQLPVNEFFALRLLSKNINQ
                     LFNNQLFIKLIHRYFTPIKFHVPLEQLDAKQLIKELIESFQDLDTLECFEDIDTLGVI
                     DTKKVLVWDLHLAIASGDEQKIRRLFDLIPFHYFSTNERSQNDRNYYLDFSLFAMAAC
                     QPLPIRQLIFDLLTRGLNDSTDTLVDKHGFSKFDYAAILNLVDVLECSTSDDMIVHNN
                     ISPLRLACRYGQLDAVQSIINKFEWITFPINALKEASTSGHLDVVRYLCEMRNRLKNF
                     QVDDLNESLLAACKNGHLEVVRHLLDLGASIEAKDNEGNTPFILACQRDHLAVAKLLI
                     TKGAKPNAKNRSMEHVLNVVLKPRYGVASNEMIKFLLNLDLDLSNQDNEHIIPLRRAF
                     QWGNIEIIDIISQKAPITPSDAQEILHSMAWPGYIINENVIDYLIQKGANPNENEASY
                     GRSPLIAAIINNNLKLIRALFNKGADLNKLHEETGLSPLHYACSNSNLETIELLLELG
                     ANPNIKDKNGNGVFLKLEDRRKDRGSILQALIKKGFNINEPSCYGETPLLRAYSSIIS
                     IFFDKKEDLVSILLDHNADLNVVCKNPFFKGQSLFALACQGSDFTMIKRIFKSNENVL
                     LSNKQHQFSEMIGKHYIQSPDMAPLLKHFDRINNQNIAPHRYIYNLLAQYSRSDCDSN
                     YSAMFARFFTGHWGNHFGDLVYETLKKTKANKPFASVKDVSIFFQELSQTSSRSQLAN
                     RNRNGGTLHSIISLANLLMDTEFDDLPLTKSQAVVRDIQEDLTNFNLR"
BASE COUNT         1871 a         1133 c         1286 g         1908 t
ORIGIN      
        1 ttcgggacaa ttgggacaac cgcataggca ctcggttttc tgtgtcccaa tatcaatttt
       61 tcacttggga cacatgggac aaatttttct ggctaaattt ttttagtaac aatgaatatc
      121 atttaataac aatcagatca caaggcttta aagcgcttga caccctttta aattacacac
      181 aatacttctc gcgagctgta aatttttacg ggtctacagt accgtaaaaa caaaggaaaa
      241 tgaaatatca cgactttatc cctcttaagg gaaccggccg gtcagtcaac tgatattcat
      301 cgagcaggac gcaaaccgtc ggcattaagc tgaatgaata atcatttgat gaggttctat
      361 atgaataaaa aattatctcg tttacaactg ttaaacgaat ttgagtctgc accaacttct
      421 gcgctgttta atcaacaaac attggcggct gttttagact gttctagcca acttttggaa
      481 cgtaatcgtt gggaaggaaa gggcattccc tatcttaaaa taggccgtaa ggttttgtat
      541 cgcaaaagtg acgtattgtc ttttctccaa caacagaaaa tctatcgatc tacctgtgac
      601 gaagggcaag tcctttcagt ggccaaccaa taaaacagat ggaaatttgt cccattattt
      661 ttaagtgatg cgacaaattg ggacggctcg aacccagtgt ttacgcgctg tcccgcctgt
      721 cccaaatgtc ccgctaattt ttgagatata accacagagt attccgcgat tttattccca
      781 tctcatcacg tatcagccaa caaatcttgt aatttccata gttaggcaga aggtacgcat
      841 acatttgttt ggcatacaaa tatcgccagc ttttgctgct ggataaacag tgcttggcta
      901 atgcactgcg ccagatgatg cggatttcct cataagattt taattgaagc agattaccgt
      961 attgctttgt gagccagtta aaaagattga gtactgcttt ctggctttca gttcgaatcg
     1021 gtatgcttcg gtcggtggag ttaaaagcaa tgtctcgggt tatccaaagc gagccttctc
     1081 taacttggat atgtggctta atcagaattg cttctttgaa agtaagtcca aactcggttt
     1141 gtaatcccat aatgactcgg gctattgggt cgtgaaggga cttccaaata tcaggaggga
     1201 ttgttttctt tctctttttt cttgttttag gtcttgatag ttcaagcgat tggttatcga
     1261 tatcggcaac agaacaatcg ttcatctgta aaaagcggcg gataatagtc atataacgca
     1321 tgatggtgac tgggttaata ttctgttttt tccagtaacg caccagcttg tgaatctgct
     1381 cagactgcaa ggcctgccat gaaggtggaa cctgtcggat aacaaacaaa tcatcaatca
     1441 tcttacgaat gacataagca cggtgtttcc tgtacaggta tttaccttgc ctgtccagtt
     1501 taatatatcg attagcgaat tgtcttaagg attgtgtacg catcttccca tctgcaaaaa
     1561 ttaatcaaaa aagaatttgc cctgtgtatt gtttagtgtt ggtcgagcgg ttcactctct
     1621 gttgaaaccc ggtaaaaccg taaagctcga agaagggggc aaatcaatcg ttataaggca
     1681 aacgatcccg cctgtactgc tagtcttttt gtgtgttttt ctcctcaata aataaagtaa
     1741 atgaaagtga taaagcaggg ctttatctca aatccatggt caaatgaaat gacttagagt
     1801 tccgaagaag tccccttacc caacggtaaa ggttcctgaa gggtttcccg actatacgtc
     1861 cagccacaag gttagggaat gagagtggct tagcataaga cgcctatgcg tttcatcgga
     1921 ttttcaccga ttcatcagtc atgcttcatt taaaaaatat caaagtgcag atctgggagc
     1981 aataccattt cagggaatat tttgtcctat cttatctggt gcgtcaggac aaatggtgtt
     2041 ttaaggcttg gtgttggagg ggtagtgatg gcgaatattc ctgtgtcact gctggaaaat
     2101 tgcagtgacg cagaaaagca ttctaatgaa ttgaggactt gttgttaaat aaatttatgt
     2161 gttaggttga ggtacgcaat ggaaagcgaa gcaacctatg tccaacactc cccataccca
     2221 aaatgggcta ttggcgcgca cctccatgga atatgcacgc catcgacccg agcaaacctt
     2281 actttatcaa gtgattgagg agtattatcc tgcatttttg tcccatcttg ctgaattaga
     2341 aaagtcagta ccccagtatg ttcaagatga atttgagtcc tacctgaaat gtgagcgact
     2401 tgagcatgga tttttacgtg tacaatgcga atcttgccat gttgagcaac tggttgcatt
     2461 tagttgcaag cgccgaggtt tttgtccgag ctgcggggca aaacggatgg tggagagcgc
     2521 tgctttgcta gtggatgaag tattaccaga aaagccaata cgtcagtggg tattgagtgt
     2581 gccttatcct ctacgttggt tatttgcaag cgagcctgag atgatgagta aggcactatt
     2641 aatagtgaca agagctatca gtagctattt gattaagaaa accggattca ctcacaagac
     2701 agccaagaca ggtgctgtga ctttgattca acgttttggc agtgctttga atctgaatat
     2761 tcactttcac atgttatttc tggatggggt gtatggagtt gattcaggag gcgccattgg
     2821 agcgtttcac gggattaaac cacctagcag caaagagatg agtttattgc taggcaagat
     2881 tagtgagcgt atagcaagac tagttgagca agcaggctat ttagagaggg atgagggtga
     2941 tggtctgaaa ctggaaggtt ttgaagatga aataatgaac cactttcaag gcagttcaat
     3001 tacctacaaa attgcggtgg gttcccagaa aggacggaag gtattgacga taaagacctt
     3061 accgtgcaag ggttcaaaaa tgagttgggc gatgcggtta aagcgagtgt ttaacattga
     3121 cataacggtt tgcaggcatt gtcagggtag ggtaagaatc attgcttgta ttgaagagca
     3181 agtggtgatt gaccagattc tggcgcatat gaatcaacag caacaatcgc ggatattagt
     3241 ccaagtttca tcttggatcc ggacaccgcc aattgagcag tcaagtcaaa tgacaatgaa
     3301 ttattagtga ctttagtcaa atcaaaagaa catggaatca gcatgaggct tgctgtcatc
     3361 ttggatttac aaatgagcat aaatcaaaca tcaaaatgag gtgttttctg gatctagtgg
     3421 gagcttgttc atgaatgcag cctgtttatt aataaaaatg ggtggtttct tgctaagaat
     3481 ttaaatagga tacttttgaa aaatgggaaa taggtggttt aaaattccta taccctaatg
     3541 ctgctttgag ttaaatgtag gttggtgctg agcttgcgaa gcccaacaag ggagcgtagc
     3601 gacctccttt acgttgggct gcacttcgtt tagcaccaac ctactttttt gatttacctt
     3661 aggttaaaat tagtcaagtc ctcttgaata tcgcgaacaa ccgcttgtga ttttgttaag
     3721 ggcaaatcat caaattcagt gtccatcaac aaattcgcta aactaataat actgtgcaaa
     3781 gtaccaccat tcctatttcg attagccaat tgcgatctgc ttgaagtttg cgacaattct
     3841 tggaaaaaaa tgcttacatc cttgacagaa gcgaagggtt tattcgcctt tgtctttttc
     3901 aacgtttcat aaacaagatc accaaaatga ttaccccagt gcccagtaaa aaatcgcgca
     3961 aacattgcac tgtaattaga atcgcaatct gaacggctgt attgagcaag taaattataa
     4021 atatagcgat ggggtgcaat attttgattg ttaattctat caaaatgttt caaaagcgga
     4081 gccatatctg gactttgaat gtagtgttta ccaatcatct cagagaattg atgttgctta
     4141 ttgctaagta aaacattttc attcgattta aatatccttt taatcatagt aaaatcggac
     4201 ccctgacaag ctagggcgaa taaagactga cctttgaaaa aaggattttt gcaaacaaca
     4261 ttaagatccg cattatggtc aagaagaata gatacaagat cttctttttt atcgaaaaaa
     4321 atagaaataa tagaagagta agcgcgcagt aatggagtct cgccgtaaca agacggttca
     4381 ttaatgttaa atcctttctt gatgagtgct tgtaatatag agcctcggtc ttttctacga
     4441 tcttcgagct ttaaaaatac accattacca ttcttgtctt tgatattggg attagccccg
     4501 agttctaata aaagctctat tgtctctaaa ttactattac tgcaggcgta atgtaatggg
     4561 cttagtcctg tttcttcatg cagcttattt aggtcagctc ccttattaaa tagtgctctg
     4621 atcagtttca aattattatt aataatggcc gctataagag gactcctgcc ataggaggcc
     4681 tcgttttcgt ttggattggc tcctttttgg attaaatagt cgatgacatt ttcattgatg
     4741 atgtaaccag gccaggccat tgagtgaaga atctcttgtg catctgatgg agtaatcggt
     4801 gctttttgcg aaattatgtc tataatttcg atgttgcccc attgaaatgc acgccgtaac
     4861 ggaattatat gttcattatc ttgattggat aaatctaagt ccaaatttaa caaaaatttg
     4921 atcatttcgt ttgaggctac tccatagcgt ggttttaaaa ctacattaag aacatgttcc
     4981 atagatcggt tctttgcatt aggctttgca ccctttgtaa ttaaaagttt tgcaacagct
     5041 agatgatctc tttggcaagc taaaataaag ggggtattcc cctcattatc cttagcttca
     5101 atacttgctc ctaggtctaa taaatgccta actacttcta aatgaccatt tttacaggct
     5161 gctaaaagag attcatttaa atcatcaact tggaagttct tcaatctatt cctcatttca
     5221 catagatatc gaaccacatc caaatgtcca ctcgtactag cttctttgag tgcgttgata
     5281 ggaaatgtta tccattcaaa tttgttgatg atgctttgga ctgcatcaag ttgtccatat
     5341 cgacaagcta accttagtgg tgagatgtta ttatggacta tcatatcatc tgatgtacta
     5401 cactctaaaa catcgacaag attcaaaatg gcggcataat caaatttaga gaaaccatgt
     5461 ttatctacta aggtgtcagt agagtcgttt aaacccctcg ttagcaaatc aaaaatgagt
     5521 tgcctaatag gaaggggttg gcatgctgcc atggcaaata aggagaagtc tagatagtaa
     5581 tttctatcat tttgagatct ttcattggtg gaaaaataat gaaatggaat caagtcaaaa
     5641 agcctgcgaa tcttttgttc atcaccactt gctatagcta gatgtaaatc ccaaactaaa
     5701 accttctttg tgtcaataac gcccagtgta tcaatatctt caaagcattc taatgtatcc
     5761 aaatcttgaa agctttctat aagctcttta attagttgtt tagcatcgag ttgttctaaa
     5821 ggaacatgga acttgatagg tgtaaaataa cgatggatca attttataaa cagttgattg
     5881 ttaaacagtt gattgatgtt tttgcttagc aggcgaagcg caaaaaattc attcaccggt
     5941 aattgtgcaa ataagtcaac taagaaatcc tggttaaaaa ttgttggcaa taaatcggta
     6001 ttatcagttt tgttaagcat tttctaatct caataagtta ttaaatcatg tccaaaggac
     6061 acaaacgaat aattgggatt tttgctcgac aactgtgcaa aaagtgtttg aatgatagga
     6121 gttcaaacct tattgatagc tagttgtttt agagacaaaa attaagaaaa gaaaaaatct
     6181 ggaataatct tatgactt
//
LOCUS       DAXLDT010000168         6189 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXLDT010000168 DAXLDT010000000
VERSION     DAXLDT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.168"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..306
                     /locus_tag="ACF2SP_002633"
     CDS             <1..306
                     /locus_tag="ACF2SP_002633"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFD2360404.1"
                     /translation="VSNILKQTLRRSSDSMFRIGGDEYAAILINMTPNEVTTVCVSMQ
                     EQFNKKNQYKNVSLSMGVISIDSMRVIDQQSAITAADKTLYQAKKAGKNQIKSKSLG"
     gene            complement(617..1003)
                     /locus_tag="ACF2SP_002634"
     CDS             complement(617..1003)
                     /locus_tag="ACF2SP_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360405.1"
                     /translation="MKKWTVLAASSLLMVNAYANESFCGYKDFFHLGDKTHPGIYVVS
                     GYNDSDVVLQIVGPRSFVIRDGFDCRAGYAHVTVAYDNANWCVLDINDGPFMNHPVVS
                     ASCNGLRYINTTYDGFGSYSYSINIE"
     gene            1260..1859
                     /locus_tag="ACF2SP_002635"
     CDS             1260..1859
                     /locus_tag="ACF2SP_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0375 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360406.1"
                     /translation="MYSSLFLKKDLGRLPEKVIFLDFDGTVALPARKENGEYDIQPDG
                     SIGATHLNKEKFRRLVQFAIHTDIPLYFLSGRPDLQSCVDLMTDFIASVDGFHSGIGG
                     FKKDSLFFISKMIIEEGKLVRKEITTKAQVIQSVHHEKYSYLPKESILLVDDIQEYLD
                     PAQELGYNTLLANPESLEHFDEVEKFIGVTLQKTIAFNT"
     gene            complement(1917..6188)
                     /gene="sdhA"
                     /locus_tag="ACF2SP_002636"
     CDS             complement(1917..6188)
                     /gene="sdhA"
                     /locus_tag="ACF2SP_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhA"
                     /protein_id="HFD2360407.1"
                     /translation="MISEKIKLLESFKQNQFSKLIEKINSKILATLKSGHFNPRLAPY
                     LEKMNKDGVPFTDFDLDPENISQIKKLINALYHARLAFLDLENIDIGNIKRTIPDLKL
                     LYSNTIHQAYQAGYLITNLDVDLQEMFKEEIDLILPVIGKLQAFAGEHQECTKRLAES
                     LKDFPISYKAGEVTGIALEQMQPRTGDWDYQFLTQFSAVLPGYIEKLTQYIQQYSSQI
                     KEKEPTLNNEKLEELQNASLKLLNDLEHLKGNDFFISLKVLKYIHIIRNIITLATSSV
                     EQMGHFSDSSQDVIRDNLAQLKYVVLPTLFGLVDKIEDNAMLKPGTLSIPLMEKIKPL
                     YQLLIYYASKPVNFKEKGEELLSIEDSRFLALRLERTYQRIDEANKALFKIQKAQEAL
                     DNFYKILDTPPYNKQPIHQLPKEIKNQLITYYKIISPYMMDVDPDLNILLIDSFQGPE
                     SWSSYLKKPWRWLRGSLPADHASFVLAQKEALQTLISKKKATQQFHIELNRDLIESVH
                     KQTNLVLFPYSEKTNVFLLDETKALNPANGATDKLKFRPEKGHNILVNPENLTSEQAL
                     DLYQWYRNKREKFRVASKAYNEFITLLKKQTKTIAETEGRILHLNNLDKETKTRCRNL
                     YNIFQPYFIDGIPPELRASAISFDKFLVHSFSNESSTYDAPAVDLFEKLDEHFQIYFT
                     EIDLQWGKKSNQYLKWAQEKFASENNSTELEHDEKLESRAHHLIKHTNFSKCIHEFRT
                     ELNQVISFFNNAMRAQLKQQSNGVPYPELQDQNKELAQSEQVVAIKRIFNSLYHVEKI
                     VVELEKLDTRSYESIYVYHLVQAYDHINEIKKLALKLAVDPHFKLIASELLEKAQTLI
                     ATIQEHSDAYQVSPLEVPYNQDIKYNALWYTLNAFYISPKHIRSLRNTNYLTTEELND
                     LHLKAKKATVTVENIIKSSNSYFKLFLQTPAMYSLYREMTNKLNEFISTSHDAVMNNL
                     DQFRSKIFTPMLMEADAWEDKLGLVPGTISGPLKTITDEYYKGLLHPLALHSKAHIRM
                     ICDKTPIDQRIKTTNKKLENATQHLDKIEKNYKHIIKLYEIIKTKGGKTTDDELIETY
                     KKALYKLVKLQKRLPQNTDHDPKDYELDTLLNSELKEYEPKLTLIKPLIISSYHHYEG
                     LKATYLMKANTAREKLSYLKELRSTQEQEDLLYIEEYTTESFNKQLDAFCNRHIGLQY
                     TDKEYRTKLREYLLTFKESIIQRSKSAEDINLTIKNLLKEKIKNFERDNFEKYYHLDA
                     VRVALAQFKNYFSLSTAAIEQNNSTFESEETLAKKTELINKLVDISEDGTLTPKERVD
                     QISFHVKNPNFERIIMDYKQESYFSFTYLKQCILSLLEALCLYTPERRKLFNNLESSV
                     KTQPKINELTKRFGLFAGNESAISTESRAITPVSSIENNGTIELN"
BASE COUNT         1794 a         1003 c         1201 g         2191 t
ORIGIN      
        1 gtttcaaata tcctgaaaca aacattacgg cgctccagcg atagcatgtt tcggattggt
       61 ggtgatgaat atgctgccat tcttattaat atgacaccaa atgaagtgac aacagtttgt
      121 gtttcaatgc aagagcagtt taataaaaaa aatcaatata aaaatgtgtc gttaagtatg
      181 ggggtcataa gcattgactc tatgagggta attgatcaac aaagtgccat tactgcagcg
      241 gataaaactc tttatcaagc aaaaaaagca gggaaaaatc aaattaaatc taaatcgtta
      301 ggctagcaag aattgagagc caattaaaat tgttggattt gattacagca agtatgagat
      361 aagagagtac aattttattt ctcttatgtc agagacggcc tctggattaa ttacttttaa
      421 cgtgagttat ggataagatg ctcgttatct tggattgttt gggccgagga agcatttatg
      481 ccgtctcgaa gccttgtatg aaatttaatg attcttgcca gggcttcgag aagatgctca
      541 tgtatcttct cagcccaaat ggtccctgga tatcgattgc ttcttatata atcgctatcc
      601 atagctcacg ttactttcat tcaatgttga tagaatagga gtaacttcca aagccatcat
      661 aggtggtatt gatgtaacga agaccattgc aggaagcact gacaacagga tgattcataa
      721 aaggaccgtc attaatatca agtacgcacc aattggcatt gtcataagct acagtgacat
      781 gagcatagcc tgctcgacaa tcaaaaccat ctctgattac aaagctacgt ggtccaacga
      841 tttgtaatac gacatctgag tcattataac cactgaccac ataaattcca gggtgagttt
      901 tgtcacctaa atgaaaaaaa tccttgtacc cacagaatga ttcattggcg taggcgttga
      961 ccattaaaag gctacttgct gccagtactg tccatttttt catacgaaaa taccttctta
     1021 ttgttattca ggaaatcgaa ttatagggaa tcgatggcta ttaaacaatc atcttgttct
     1081 tttgtttttg aaaaacctgt tctgcaagtg atttctggaa agggtaggaa atatttcctg
     1141 ttaaagagat gatgagttac cttgtttcga ttgatctaat cggatactct gagcagttga
     1201 tattgagaat ctctacaata ttttgaaggg atagaaacat taaattgtca ggaggaaaaa
     1261 tgtattcatc attatttcta aagaaggact tggggcgttt acctgaaaaa gttatttttc
     1321 ttgattttga cggcaccgtt gctcttcctg caaggaagga aaatggagaa tatgatattc
     1381 agcctgatgg gtcaattgga gctacccacc taaataaaga aaaatttaga cgtttagtac
     1441 agtttgctat tcacactgat attcctttgt actttttgag cggacgtcct gatcttcaat
     1501 cgtgtgttga tttaatgact gattttattg caagcgtcga tggcttccat tctggtattg
     1561 gcgggtttaa aaaagattct ctgtttttta tcagtaaaat gataattgaa gaggggaaac
     1621 ttgttcgaaa ggaaataacg acaaaggctc aggttattca atccgtgcat catgaaaaat
     1681 acagttattt acctaaggaa tcgattttac tggtcgatga cattcaagaa tacctcgacc
     1741 cagcgcagga actgggttat aacactttgt tggcaaatcc ggagagtttg gagcattttg
     1801 atgaagtaga gaaatttatt ggggtaacat tacaaaaaac tatcgcattc aacacctaga
     1861 caaggctatg gtaaattaaa agattaaatc aaatattttt atgctgatgg cgctaattag
     1921 tttaactcaa ttgttccatt attttcaata gaagaaactg gggtaattgc cctggattca
     1981 gtagagatag ctgactcatt gccagcaaat aatccaaatc gtttggtcaa ttcattgatt
     2041 ttaggctggg tttttacaga gctttccaaa ttattaaata gttttcttct ctccggagtg
     2101 tacaggcaaa gcgcttccaa taaggataag atgcattgtt ttaaataggt aaagctaaaa
     2161 tagctttcct gtttgtaatc catgatgatt ctttcaaaat taggattttt aacatggaaa
     2221 gatatttggt cgacacgctc ttttggcgta agggtcccat cttcactgat atcaaccagt
     2281 ttattaatga gctctgtctt tttcgccagt gtctcttcgc tttcaaatgt cgaattattt
     2341 tgctcgatgg cagcggtaga aagactaaag tagtttttaa attgagctaa agcaactctt
     2401 actgcatcaa gatgataata tttttcaaaa ttatctcttt caaaattttt aattttttcc
     2461 tttagcaaat tttttatagt taaattaata tcttctgcgg attttgagcg ttgaataata
     2521 ctttctttga atgtcagtaa atattctcta agttttgtgc gatattcctt gtcagtgtat
     2581 tgtaaaccta tatgacggtt gcagaaagca tctaattgtt tattaaatga ttctgtagtg
     2641 tattcttcta tataaaggag atcttcttgt tcctgagtcg atcgtaactc tttaagataa
     2701 ctcaactttt ctctggcagt atttgctttc ataagataag tggcttttag gccttcataa
     2761 tgatggtagc ttgagataat gagaggctta atgagcgtta atttaggttc atactctttc
     2821 agctctgaat ttaaaagagt gtctaattca taatctttag gatcatgatc tgtattctgg
     2881 ggtaatcgct tttgtaattt taccaatttg tacagagctt ttttataagt ttcgattaat
     2941 tcatcatcag tggttttgcc gccttttgtt ttgataattt catatagctt gatgatatgt
     3001 ttataatttt tttctatttt atccaaatgt tgtgttgcat tttcaagttt tttattcgtt
     3061 gtttttattc tttgatctat tggtgtttta tcacatatca ttcgaatatg tgctttggaa
     3121 tgcagcgcta aaggatgtaa caggccttta taatattcat ccgttattgt ttttaatggt
     3181 cctgagatag tacctggtac caagcctaat ttatcttccc atgcgtccgc ttccatcagc
     3241 atgggtgtaa aaatcttgga gcggaattga tccagattat tcattacggc gtcatgtgat
     3301 gtgctaataa attcattcag tttgttcgtc atttctctat agagagaata catggcaggc
     3361 gtttgtaaga ataatttgaa ataggagttt gaacttttaa tgatattttc tacagtaacg
     3421 gttgcttttt ttgcctttaa gtgtaagtcg ttgagttctt ctgtcgtgag atagttggta
     3481 tttcttaaac ttctaatgtg ttttggtgaa atataaaaag cattgagtgt ataccagaga
     3541 gcattgtatt taatatcctg gttataaggt acttctaagg gggatacctg atacgcatca
     3601 ctgtgttctt gtattgttgc aataagagtt tgtgcttttt ccagtaactc gcttgctatg
     3661 agcttaaaat gaggatccac agcgagtttt agcgctaatt tcttaatttc attaatgtga
     3721 tcatacgctt gtactaaatg atagacataa atgctttcgt aacttctggt gtccagtttt
     3781 tccagctcaa caacaatctt ttcaacatga tagaggctat tgaatatgcg ttttattgca
     3841 acaacctgtt cactctgggc aagttctttg ttttgatctt gcaattcagg gtaaggtact
     3901 ccattggatt gttgttttaa ttgtgccctc atcgcattat taaaaaacga aatgacttga
     3961 ttaagctccg ttctgaactc atgtatgcat ttagagaaat tggtatgttt aattaaatga
     4021 tgggctctgc tttccagttt ttcatcatgt tcaagttcgg ttgagttatt ttcactggca
     4081 aatttttcct gggcccattt taaatattga ttactctttt taccccattg taaatcaatt
     4141 tctgtaaaat aaatttgaaa gtgttcatcc agtttttcaa acaggtcaac tgccggagca
     4201 tcataggtgg atgactcatt agaaaaggaa tggaccaaaa atttgtcaaa acttattgcg
     4261 gaagctctga gttcaggagg tatcccatcg ataaaataag gttgaaatat attatataaa
     4321 ttacggcagc gggttttggt ctctttatca agattattta aatggagaat cctgccttcc
     4381 gtttcagcga ttgtcttggt ttgctttttt aataaggtaa taaattcgtt atatgctttg
     4441 ctggcaactc tgaatttttc acgtttgttt ctataccatt gatagaggtc aagagcctgt
     4501 tcagatgtta aattctcagg attaaccagt atattatgtc ctttctctgg cctgaatttt
     4561 agtttgtccg tagcgccatt agcagggttt agtgcttttg tctcatctag aagaaagaca
     4621 ttcgtttttt cactataagg gaataaaacc agatttgttt gtttgtgaac agactcgata
     4681 aggtctctat tcaattcgat atgaaattgt tgagtggctt tctttttgct aattaaagtt
     4741 tgtaatgctt ccttttgcgc taaaacaaaa ctggcgtgat cggctggaag actgcctctt
     4801 aaccaacgcc atggcttctt taaataagaa gaccaggatt ccgggccctg aaaactatcg
     4861 atcaacaaaa tattgagatc aggatccaca tccatcatat aaggactgat tattttgtaa
     4921 tatgtaatca gttggttttt aatttctttc ggtaattgat gtatgggttg tttattatag
     4981 gggggggtgt ccagtatttt gtaaaaatta tccagagctt cttgcgcttt ttgaatttta
     5041 aaaagggctt tgttagcttc atcaattcgt tgatatgttc tttccagtct taaagcgaga
     5101 aaacgggagt cctctatact gagtaactct tctccttttt ctttgaagtt gacgggtttg
     5161 gatgcgtaat aaattaaaag ttggtataga ggcttgattt tttccattaa cggaatggac
     5221 agtgtaccag gcttcagcat ggcattgtct tcaattttat ccactaaccc aaataaagta
     5281 ggtaatacaa cgtattttaa ttgagccaaa ttatcacgaa tgacatcctg ggatgaatcg
     5341 ctgaaatgtc ccatttgttc aacagagctt gtcgccaatg tgatgatatt acggataatg
     5401 tgaatgtatt ttaaaacttt aagggatata aaaaagtcat tccctttcag atgctccagg
     5461 tcatttagta attttaatga tgcgttttgt aactcttcca gcttttcatt atttagggta
     5521 ggctcttttt ctttgatttg tgaagagtat tgttgaatgt attgagttaa tttttctata
     5581 taaccaggta atacggcact gaattgagtg aggaattggt aatcccaatc tcctgttctc
     5641 ggttgcattt gctctaaagc aatgcctgta acttctcctg ctttatagct tataggaaaa
     5701 tccttcaaag actctgccaa acgcttggtg cattcctggt gttcaccagc aaaagcttgt
     5761 aattttccaa tgacaggaag aataagatca atctcttcct tgaacatttc ctgcaaatca
     5821 acatccaggt tagtgattag ataaccggct tgataggcct ggtgaatcgt gttgctataa
     5881 agtaatttga ggtcagggat agttcgtttg atgttcccta tgtcaatatt ctctaaatcc
     5941 agaaaagcta aacgggcatg gtaaagggcg ttaatgagtt tcttgatttg agaaatattt
     6001 tccgggtcaa gatcaaaatc ggtgaatgga accccgtctt tattcatttt ttccaagtag
     6061 ggggccagtc tggggttgaa atgtccactt tttaaagtcg caaggatttt tgaattaatt
     6121 ttttcaatta atttagagaa ttggttttgc ttaaaggatt ctaaaagctt gatcttttct
     6181 gaaatcatt
//
LOCUS       DAXLDT010000169         6175 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXLDT010000169 DAXLDT010000000
VERSION     DAXLDT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.169"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            303..2027
                     /gene="pilB"
                     /locus_tag="ACF2SP_002637"
     CDS             303..2027
                     /gene="pilB"
                     /locus_tag="ACF2SP_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFD2360408.1"
                     /translation="MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQ
                     YIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGAIPVNLVNEKLIKRHAMVPLFSRG
                     TNLYLATDDPSKQASLKEIQFHTGLNTHAIVVEADKLSALIENLLTAKESQGLSEYVE
                     DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYERE
                     YRIRYRQDGILHEVATPPASLSSRITARIKVMSNLDISERRIPQDGGFKMKISKSRAI
                     DFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGP
                     TGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFL
                     RQDPDIIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIA
                     SSVTLIIAQRLARKLCNQCKAVRDDFTNQGLIELGFKEADLVNLKLYKAVGCEQCTSG
                     YRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEGITTIE
                     EVNRVTVD"
     gene            2033..3253
                     /locus_tag="ACF2SP_002638"
     CDS             2033..3253
                     /locus_tag="ACF2SP_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFD2360409.1"
                     /translation="MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGI
                     VTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMIESGIPLVQAFDIVAKGQSNKRL
                     KDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
                     IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITM
                     SEFMQAYWYIIFGALGGVVYSFFYAKNHSLEFAQTIDRVMLKLPVIGPILEKAAIARF
                     ARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATGQQMFIAMENTHLFP
                     NMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLV
                     VAMYLPIFKLGSAV"
     gene            3299..4162
                     /locus_tag="ACF2SP_002639"
     CDS             3299..4162
                     /locus_tag="ACF2SP_002639"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFD2360410.1"
                     /translation="MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWK
                     EQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYIFLRGRCYQCDSP
                     ISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
                     GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAA
                     FGAWLGWMYLPLILLISSITGAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGD
                     SIINWYIGYWM"
     gene            complement(4176..5381)
                     /locus_tag="ACF2SP_002640"
     CDS             complement(4176..5381)
                     /locus_tag="ACF2SP_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFD2360411.1"
                     /translation="MNPNDASKKDNGNSNQLDTELIGSYQSITFGFSKDELASFSKIG
                     QHKHYTAGSIVYNENDLADSFFIIIDGVAEIFKKTIDEFDEHNIHTLATLSKDDVVGE
                     MALIENSRRSASVKAKTDLSVIEYPLEVVKAKPKLNLMLIKNMAQILSRRLRFTNEVT
                     VKNMQQALKEASGRNTLGVFIIALLWIICLYTLSLHFLVTVIHQVTVTTVLSICLISI
                     FAVAILIAMHFTGLPYSRFGITAKDWGKTILEALAFTIPIMLFVLIIKVFFIIGIDHL
                     DNTPIFSGTKRFLVNNEVDWEYYFAMIFLYSLFAPIQELMARSALQSTFFHFLPGEKL
                     FRQWNGIFLSNLIFATMHTHLGLTFASLTFIAGLFWGWLFHRQKSLLGVSVSHVILGV
                     WSLFIVGLR"
     gene            complement(5489..6082)
                     /locus_tag="ACF2SP_002641"
     CDS             complement(5489..6082)
                     /locus_tag="ACF2SP_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360412.1"
                     /translation="MFIFKRWKIRKITKRIKAMQANRVSNQPGDEVLKKEILYYFELA
                     TIFKKLKNHKKYPYAEIMMIECYRAAANLDDSAANFQLGQIFLDEAKYRQKLDNEGIF
                     NSQANLKRAQQLFDEAHAHLIAAEKLGHVGAKRLRGLCIINGWGVESDKNAGFELVVD
                     SIEQEGSWDKIPQIFASMGLNKPEFFSAIMQRRKGTS"
BASE COUNT         1971 a         1047 c         1179 g         1978 t
ORIGIN      
        1 tcgtataaag tgcgataatg ttttatttga tgttcaatca acaaaatgtg acctttaatt
       61 tattaccttt tattaactac aatacaacga gttttagatg caaacaattt aaatctttca
      121 aattttttat tatttccaag agagttgcgt tatagcccgt gtttgcaaaa aattgatatg
      181 gcaaagtaac cagaaatagg atttaggggc agttccaaaa tacctggttt tgttcaaaaa
      241 accatgcaat tataaaaaaa tttaactata tttaaaacta agatacactt tgaataaatg
      301 aaatggctct agctacagaa gaatatagat tacagggaat cggccagctt ctcgtgctgg
      361 aaaagctgct agacaaaaca aaagcaattg aactacacaa gttggcagca gcagaaaaga
      421 tatctttgct gcaatacatt gtaaaaaata aaatattatc tgctgaacaa attgcactga
      481 cggctgctca aaattttggc gtgcccatgc tggatattaa ttgcatcgat gtgggcgcta
      541 ttcctgtcaa tctcgttaat gagaaattaa ttaaacgcca cgccatggta cctcttttta
      601 gtcgcggcac caatttatat ctcgcaacag atgatcctag taaacaagct tcattaaagg
      661 aaattcaatt tcataccgga ttaaatactc atgcgatagt tgtggaagca gataagctta
      721 gtgctctgat tgaaaacctg ttaacagcaa aggaaagtca gggcttatca gagtatgttg
      781 aagactctgg agatctggaa ggtttggaaa taagtgctga tgatgaagat caagatattg
      841 atactgcaac atcagtaact gatgatgccc ctatcgtaaa attcgtcaat aaaattttat
      901 tggatgcgat aaggcaagga gcttctgata tacactttga gccttatgaa cgagaatacc
      961 gaatcaggta caggcaagat ggtattttac atgaggtcgc tacccccccg gcaagcttgt
     1021 catctcgtat tacagcacgc atcaaggtaa tgtctaattt ggatatatca gaacggcgta
     1081 tcccccagga tggtggcttc aaaatgaaaa tttccaagtc acgagcaatt gatttcagag
     1141 tgagtacatg tccaacttcg gctggggaaa aagtggttat gcgggttctt gattctggtg
     1201 ctgctaaatt aggtattgaa gcattaggat ttaatcctgt tcagcgaaca aatttcctga
     1261 aagcaatcca gcgcccacaa gggatgattt tagtcactgg ccctactggt agcggtaaaa
     1321 cagtcacttt atatacggct ttaaatatat taaatacaat tgaggttaat atttcaacag
     1381 ctgaagatcc agttgaaatc aaagtgcctg gtatcaatca ggttaatatt aatcctaaag
     1441 ctggcctaac cttttccggt gcattgcgtt cgtttctaag gcaagatcct gatattatca
     1501 tggttggtga gatacgtgat cttgaaacag cagagattgc tataaaagct gctcagacag
     1561 ggcatttggt tctttctact ctgcacacaa acagtgccgc tgaaacttta aatcgtttag
     1621 taaacatggg aatacctact tttaacatcg cgagttcggt taccttaatt attgcgcaaa
     1681 ggctggcaag aaaattatgc aatcaatgta aggcagtgcg tgatgacttt accaatcaag
     1741 gcttgattga actaggtttc aaggaagctg atttggttaa tcttaaatta tacaaagcag
     1801 ttggttgtga gcaatgtaca agtggttatc gcgggcgagt tgggttgttt gaggttttac
     1861 cgatgacgaa agagcttggc caattgatta tgtcaggtgg taattcgctg gatatattaa
     1921 agttagctca atcagaaggt atgctgacta tctttcaatc gggtatagaa aaagttaagg
     1981 aaggaatcac gaccatagag gaggtcaatc gggtaaccgt tgattaattt ggatggataa
     2041 aaatagccca cccctattaa cctttcatta tcaaggcatt aataaagccg gtcaaaaaat
     2101 ggagggtgat attcaagcca gaagtttggc aatagctaaa gctgatttgc gtaagcaggg
     2161 gattgttacc aataaagtag taaaaaaaag aaaacctttg tttgatagaa aaaataaaaa
     2221 aatcacacaa gcagatatta cagtgtttag ccgtcaatta gcgacgatga tcgagtctgg
     2281 aataccatta gtgcaagcgt ttgatattgt tgccaaagga caaagcaata aaagacttaa
     2341 agacttaata gaaacaatta agcacgatat cgaaacgggg ctaacgctag cagaatcctt
     2401 aattaaacat ccactctatt ttaatgaatt attttgtaat cttgtcgatg caggcgaaaa
     2461 atcaggctca ttagatatca tgcttgacaa ggttgcaacg tataaagaaa aaattgaaat
     2521 cataaaaaag aaaataaaaa aagctctcac ttacccaatt gctgttatgg ttgtcgcact
     2581 tttggttact gcaggattat taatctatgt agttcctcaa tttgaatcgt tgtttaaagg
     2641 atttggagct gatttgccag cgatgactag agctgtgatc actatgtctg aatttatgca
     2701 ggcttattgg tatattattt ttggagcttt gggtggggtt gtctatagct ttttttatgc
     2761 taaaaatcat tctttggagt ttgcacaaac cattgacaga gtgatgctaa aactccctgt
     2821 aattggccct attttagaga aagcagcaat tgctcgattc gcgaggacat tatccattac
     2881 ttttgctgct ggattacccc tggttgaagc attgaaatct gtagctggag cgactgggaa
     2941 tattatttat gccaaggcga ctgataaaat cagagaagaa gttgcaacag gacagcaaat
     3001 gtttattgct atggaaaaca ctcatttatt tccgaatatg gtaattcaaa tggtagcaat
     3061 tggtgaggaa tcaggtgccc ttgaaaaaat gttaagcaaa gtagctgatt tttatgagga
     3121 agaagtaaat aatgctgtag atgccctaag tagtttattg gagccgataa ttatgtcaat
     3181 attgggtata ctggttgggg gattggtggt ggctatgtat ttacctattt tcaaactggg
     3241 ttctgctgtt taaacagagc ctatttaatt taatcatata atataggcaa acgtgtcaat
     3301 gataaatgca ctaataatca attatccatg gtttatgtat ttagtcgttg gactcttttc
     3361 tctagctgtt ggaagtttgc ttaatgtgat aatctaccgc ttaccaatta ttttgcagga
     3421 agagtggaaa gagcaatgct gtgagctatt ccatcttgaa cagcgaaaag aaaaaataaa
     3481 acttaattta tttttaccac gttctttttg ccctcattgt aaagcgatgg ttaaagcgtg
     3541 gcagaatatt cctctgttaa gctatatttt tttaagagga cgctgttacc aatgcgatag
     3601 tccaatttca attcgctacc cttttgttga gactctgacc acagttttat ccttatacgc
     3661 cagttggcat tttgggttta caatacaact attatttgct ttattagcta tctggatatt
     3721 aatatcactt gtttttattg atcttgatca tcaattactc cctgatagcc tcactttagg
     3781 gctcttatgg attggcctta ttgctaatac ccagaatata tttgttagtc ttgacgttgc
     3841 tgtattaagc tgcgctggcg cctatttagc tttatggcta ttcattaatc tgttttatct
     3901 gatgacaggt aaggtaggta tgggacatgg agatttcaaa ttatttgcag catttggtgc
     3961 ctggcttgga tggatgtatt taccattaat tctactaata tcttcaataa cgggggctat
     4021 tattggtttg atttatttaa agataaatgg taaatcaaga gatactgcaa taccatttgg
     4081 cccttttctt tgcatttctg gattaatagc tatgttttgg ggagactcaa ttatcaattg
     4141 gtatataggt tattggatgt aaaatgttga ttagcttagc gcaacccaac aataaataga
     4201 gaccacacgc ctaaaattac atgagaaacg gatactccca aaagagattt ctgtctgtga
     4261 aataaccacc cccaaaaaag tccggcgata aaagttaatg aggcaaaggt tagccccaag
     4321 tgagtgtgca tagttgcaaa gattagattg gacagaaaaa ttccattcca ttgacgaaat
     4381 aatttttccc cgggtaaaaa atgaaaaaaa gtagattgta acgcacttct ggccattagc
     4441 tcttgaatgg gagcaaaaag gctatataaa aaaatcatag caaagtaata ttcccaatct
     4501 acttcattat ttaccaaaaa gcgttttgtt cctgaaaata taggggtatt gtccagatga
     4561 tcgataccta ttataaaaaa aaccttgata attagtacaa aaagcataat aggaattgta
     4621 aaagctaatg cctccaagat ggtttttccc caatctttcg cagtgatacc aaatctggag
     4681 tatgggaggc cagtaaagtg cattgcaatg agaattgcta cagcaaagat tgagattaag
     4741 cagattgata gtacggtagt gacagttacc tgatgtatta cagtaaccag gaaatgtaga
     4801 gataaagtat agaggcaaat aatccatagt agagctatga taaatacccc caatgtattg
     4861 cgccctgatg cttctttgag agcttgctgc atatttttca cagttacttc atttgtaaaa
     4921 cgaagtctcc tggaaagtat ttgagccata tttttaatta acatcaagtt aagctttggt
     4981 ttagccttta cgacttctaa tggatattct ataacactaa gatcagtttt agcctttact
     5041 gacgcagaac gtcgactatt ttctataaga gccatttctc ctacaacatc atcttttgat
     5101 aaggttgcaa gagtatggat attatgctca tcaaactcat cgatagtttt tttaaatatt
     5161 tcggcaacac catcgattat aataaagaat gagtcagcga gatcattttc attataaaca
     5221 atgcttccag cagtatagtg cttatgctga ccaatttttg aaaaactggc aagttcatct
     5281 tttgagaaac caaacgttat cgattggtaa cttcctatta attcagtatc caattgattg
     5341 gagttgccat tatctttttt agaagcatca tttgggttca ttgctatttc aatcctaaat
     5401 atgcaagtcc acatttactc tatttaatgc aacgacaata tacacctcat aaataatttt
     5461 tgattaggaa attaataatg aataaatttc aagatgttcc ttttcttctt tgcataatag
     5521 ctgagaaaaa ttcaggctta tttaatccca ttgatgcaaa tatttgaggt attttatccc
     5581 aacttccctc ttgttcaata ctgtcgacaa ccaattcgaa tccagcattt ttatcgcttt
     5641 caacacccca gccgttgata atacataatc ctcttaatct cttggcacct acatgtccca
     5701 gtttttcagc tgcgattaaa tgtgcatggg cctcatcaaa aagttgctgt gctcttttta
     5761 agttggcttg actattaaat attccttcat tatccagttt ttgcctgtat tttgcttcgt
     5821 ccagaaaaat ttggcctagt tgaaaattag ctgccgagtc atcgagatta gctgctgctc
     5881 tataacactc aatcatcatg atttctgcgt aagggtattt tttatgattc tttaattttt
     5941 taaaaattgt agctaattca aaataataaa ggatttcttt tttaagaact tcatccccag
     6001 gttgattact tactctattg gcttgcatag ccttgattct ttttgtgatt tttcttattt
     6061 tccatcgttt aaagataaac atgccctact ccctcaccta agctgttagg tcaaataata
     6121 tttgtagtaa aaaaattatt gcaacaatta aaaccattaa gagaacgatt ttcaa
//
LOCUS       DAXLDT010000170         6145 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXLDT010000170 DAXLDT010000000
VERSION     DAXLDT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.170"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1051
                     /locus_tag="ACF2SP_002642"
     CDS             <1..1051
                     /locus_tag="ACF2SP_002642"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HFD2360413.1"
                     /translation="LPIKGLIIYSYYYLYEHYAIFNVNTQSIFSWLLLWIGVDFFYYW
                     YHRVSHRNSFFWIGHSVHHQSEHFNLSVALRQGYFQTLTSWVFYLPLALIGFPTWMFV
                     IVDSVNTIYQFWIHTESIRKMGWFEKIFNTPSHHRVHHGKNPQYIDKNYAGSLIIWDK
                     LFGTFEPEEAPADYGVTEPLDSWNPFYANIKVVKDILYYGKGLKSKWGVLRAFFMPPE
                     WIIHRLQQENSPVSKKTVIKKNTTSPGIYMLTNTAFAVFLYTYLSLVFDPAMVTSWLM
                     GALILLTLYTLGRIADGHSNTLYLEISRATLILLILLLFKNTLLLSLGCSVLFYIINY
                     ALCTTLPYKRTASSF"
     gene            1204..3546
                     /locus_tag="ACF2SP_002643"
     CDS             1204..3546
                     /locus_tag="ACF2SP_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HFD2360414.1"
                     /translation="MHTIANETPVQKNHSFDPITNILKWVLLVTAILCFLILIWGTVK
                     TYQLVPPLPDRFVTSTGETVMTSQDIVEGKAGFQRADLMDYGSLYGMGSYFGEDYTAK
                     YLVRLGELVQEELAKIRFNKAYSELTEGEQYVINKEMQNSLQRLDLNKKTSILTPAIA
                     TSIRQLQAEISQSLLNHNFMAGYTQAYSLNPDTALQTANFLIYSTLTTIARRPNQNIS
                     YTNNWPYEPSVGNTPSSGTFYWTWISFCFVFMGFGAVIYIYHRYLSDVDHGPMEPIFS
                     GFKPLTQSQRKVGQYFLVAALFLLLQIAVGAIMAHYYAERESFYGINVNTWLPFNFLR
                     DVHIQTPIIWIGLSWISAGIFMAPIISGKEAKGQGFLVDLLFYVTLLVVGGAILGNYL
                     GIMGYIDKAWFWLGNQGLSYIQLGRLWQIGFCIGLFLWSFIVFRGMWPTWDGLKKATI
                     EFWTGRIKLENLFWASTINVAVLYCFGMIPLTGIEKSFTITDFWRWWVVHLWVEQSFE
                     FFAACATAYLLMGTGLVSRKAAERTVYFESILIFLGGVIGTGHHWYWTGTPEMWIPLG
                     SMFSFIEVLPLVLLIIDAIEHRQLIKKQGTFTYNLAYLYILGAAFWNFVGAGVFGGGT
                     LNAPLINYYEHGTFLTLNHAHTALFGAFGLLALGLIYFCLRYAAGEGKTWSDRLGVYA
                     FWLYNAGLFLWIVLNFFPIGWSQLMAVYEHGLSYARSLEFYNTTLLWQWLRFPGDVVF
                     ALGALLMAYDFIVKIGPFFPKFARNRRFIAGPPKATDPSL"
     gene            complement(3737..5569)
                     /locus_tag="ACF2SP_002644"
     CDS             complement(3737..5569)
                     /locus_tag="ACF2SP_002644"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD2360415.1"
                     /translation="MSDLQQLPKTILRFSWHFIKKSPWLYALFFIAPVVMVLETNVIP
                     YALKMIIDGITNHKGGKESIFQDIAPALYLGGVAWFGVILVLRLHNWWQAYLVPQFQA
                     NIRMTLFGYLIQHSHHFFSNQLAGNLANKISDIPRSLEAIRKIVSCNVNTTFAAIVVS
                     IVLMLTINPWFAFILLSWIIIQLFISFYSAKEINKLARVNAEDKSILKGKIVDSLNNI
                     NTVKLFVQNEYEKKYVWKTQDKEVKSNKRLVLFINFFRLLVDVPVSIMLIAMIYSVIS
                     FWQRNLISTGDVVFIFATSFAIMNQMWSLSNSLCDLFVEIGIVKQALAILSQPIEVQD
                     IASAKELKVTHGEIRFENVTFRHRDGQELFTNKSLVIPAKQKVGLVGYSGGGKTTFIN
                     LILRYFDVDSGRILIDNQDISEITQYSLRKSISMIPQDTNLFHRTLLENIQYGKEDAD
                     RDEVVTAASLANCMDFIQNLPQGFNTIVGERGTKLSGGQRQRIAIARAFLHHTPILIL
                     DEATSQLDSLTEECIQESLQPLCEGKTTIVVAHRLSTLLNMDRILVFDKGAIVEDGTH
                     QELIENNGLYKSMWDAQTSGFLPVEQEDEAELAKEFDRPYLAQV"
     gene            complement(5731..6117)
                     /locus_tag="ACF2SP_002645"
     CDS             complement(5731..6117)
                     /locus_tag="ACF2SP_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFD2360416.1"
                     /translation="MYTIIICLFIAILLPYLAKVPVAYAMHKAGGYNNRYPREQQARL
                     QGFGARALAAHQNAFESLLIFATASLTALATNSVTLMTQYFAIAYIISRLFYHLFYLL
                     NWSSLRSTVWFLGVICCLSILWLSIP"
BASE COUNT         1778 a         1213 c         1193 g         1961 t
ORIGIN      
        1 gctccccatc aagggcctga ttatttatag ttactattat ttatatgagc attacgctat
       61 ttttaatgtg aatactcaat cgattttttc ctggttgctt ttatggatcg gggttgattt
      121 tttctattac tggtaccaca gagtaagcca cagaaacagt ttcttctgga taggccattc
      181 ggtccatcac caaagtgagc actttaactt atctgttgct ttaagacagg gctatttcca
      241 gacattaact tcctgggtgt tttatttgcc cttggctctg attggttttc caacctggat
      301 gtttgtcatt gtcgactctg tcaataccat ctatcaattc tggattcata ctgaatccat
      361 tcggaaaatg ggttggtttg aaaaaatatt taatactcca tctcatcaca gagttcatca
      421 tggcaaaaac cctcaataca tagataaaaa ttatgcggga agcctgatta tctgggataa
      481 actgttcggc acttttgaac cagaagaagc gccggctgat tatggagtca ccgaacccct
      541 tgattcatgg aacccttttt acgccaacat caaagtggtt aaagatatcc tgtattatgg
      601 taaaggacta aaaagcaaat ggggtgtgtt acgcgcgttc tttatgccgc cagaatggat
      661 cattcatcgc ttgcaacaag aaaactcccc tgtctcaaaa aaaacagtga ttaaaaaaaa
      721 tacgacctca cctgggattt atatgttaac caatactgca tttgcagtgt tcttatatac
      781 ttacctctct cttgtttttg atcccgccat ggtcacatcc tggctgatgg gagcattaat
      841 tttattgaca ctttataccc ttggccgaat tgcagatggc catagcaata ctctttactt
      901 ggaaatatcg cgcgcaacat tgatactttt aatattattg ctatttaaaa acactctgtt
      961 attgtcgctc ggttgcagtg ttttgtttta tatcatcaat tatgccttat gcactacttt
     1021 gccttataaa agaaccgcat cttctttctg agaagcagac gctattcaac aagaattaac
     1081 cctgtgggta ctggagatgt atggttttct aagagtcagt tttgtagcta acatgaattg
     1141 tttcttgtaa agatcctctt tataatcaat gccgaattga accactccgc taaggatagc
     1201 ctgatgcata ctattgcaaa tgaaacgcct gttcaaaaga atcactcatt tgacccaata
     1261 accaatattt taaaatgggt tttactagtc actgccattc tttgttttct aatcctcatc
     1321 tggggcactg ttaaaaccta tcaattggta cctcctttac cagaccgttt tgtgacctct
     1381 acaggcgaaa cggtgatgac atcacaagat atcgtggaag gaaaagcagg ttttcaacgc
     1441 gctgacttaa tggactatgg cagtttatac ggtatgggat cctattttgg tgaggactac
     1501 acagctaaat atctggtgcg tttgggagag ttggttcagg aagaattggc aaaaattcgt
     1561 tttaataaag cctattccga attaacagaa ggtgaacaat acgttattaa taaagaaatg
     1621 cagaactcac tgcaacgcct tgatctcaat aaaaaaacga gcatcctgac accggcaatt
     1681 gccacctcaa tacggcaatt acaggctgaa atcagtcaat cgttactgaa tcataatttt
     1741 atggccggtt atacccaggc ttatagctta aatcctgata ctgcgcttca aaccgcaaac
     1801 tttcttattt attccacttt aacaacaata gctcgccgcc caaaccaaaa catatcttac
     1861 accaataatt ggccatacga gccaagtgtt ggtaataccc cctcatcagg gacgttttat
     1921 tggacctgga tttcattttg ctttgtgttt atgggatttg gtgcggttat ctacatttat
     1981 catcgctatt taagtgatgt tgatcatgga cccatggaac ctattttttc aggtttcaaa
     2041 ccactgactc aaagccaacg taaagtaggt caatatttcc tggtagccgc gttgtttctt
     2101 ttacttcaaa tcgcggtagg agccatcatg gctcattatt atgctgagcg cgaaagcttc
     2161 tatggtatta atgtcaatac gtggctgcct tttaatttcc ttcgagacgt ccatatccag
     2221 actcccatta tctggattgg gctttcatgg ataagtgcag gcatatttat ggcacccatc
     2281 attagcggta aggaagccaa aggacaaggg tttttagttg accttctgtt ttatgtcacc
     2341 ttgctcgtag tgggtggagc catccttggc aattacctgg ggattatggg gtatattgat
     2401 aaagcctggt tttggctagg taatcaaggc ttgtcttaca tacaattagg aagactgtgg
     2461 caaatcgggt tttgcattgg attgtttctt tggagtttta ttgttttccg cggtatgtgg
     2521 cctacgtggg atggattaaa aaaagccacc attgagttct ggacaggtcg catcaagctt
     2581 gaaaacctgt tctgggccag cactatcaat gtcgctgttt tatattgctt tgggatgatc
     2641 cccttaacag ggattgaaaa atcgttcacc ataacggatt tctggcgctg gtgggttgtg
     2701 catctgtggg ttgaacaatc ctttgagttt ttcgccgcct gtgctactgc ttatttgctc
     2761 atgggaactg gtttggtttc cagaaaagca gcagagagaa cggtatattt tgaatccatt
     2821 ttaattttct tgggaggagt aattggtact ggccatcatt ggtactggac tgggactcct
     2881 gaaatgtgga ttccgctagg ctccatgttt tcctttatcg aagtcttgcc tctggttctt
     2941 cttattattg atgcgattga gcatcgacaa ttgattaaaa agcagggaac gtttacctac
     3001 aatctcgctt atctctacat attgggagcc gcgttctgga attttgttgg tgccggtgta
     3061 ttcggaggag gtacactgaa tgcgccactg attaattact atgagcatgg aacattcttg
     3121 actttaaatc atgcccatac tgccttattt ggcgcatttg ggcttcttgc tctcgggtta
     3181 atctattttt gcctgagata tgctgctggt gaaggcaaga catggagtga tcgattgggt
     3241 gtttatgcct tttggttata taatgcaggg ctctttttat ggattgtcct gaattttttc
     3301 ccaattggct ggtcgcagtt aatggcagta tatgaacatg gactatccta tgcccgaagc
     3361 cttgaattct ataacaccac cttattatgg caatggcttc gttttccagg ggatgtcgta
     3421 tttgccttgg gggctcttct tatggcttat gattttattg ttaagattgg accttttttc
     3481 ccgaaatttg cccgtaatcg tcgctttata gccggccctc caaaagcaac agacccaagc
     3541 ttatgaccaa tgatagcaaa gacaaagcta gtatattttt ttgaaaaatt attttgagaa
     3601 accgagttta gaagtgaata tcgacaccca aatggtgggt gtcagcttgc aatcaaattt
     3661 aaggtaagag atcagcctct acctggaacg agcgtatttg gttcatcttg aacgacaatt
     3721 gctttttttg gttaaattac acttgcgcca aataaggccg gtcaaattct ttagctagct
     3781 cagcctcatc ttcttgttcc acaggcaaga agcctgaggt ttgcgcatcc cacatggatt
     3841 tatataatcc attgttttct atcaactctt gatgtgtgcc atcttctact attgctcctt
     3901 tatcaaaaac caggattcta tccatgttaa gaagggttga aagtcgatgc gctacaacga
     3961 tagttgtttt tccctcgcat agtggttgta aggattcttg aatgcattct tcagttaaag
     4021 aatccagttg tgaagtggct tcatctaaaa tcaatatagg agtatgatgc aaaaacgctc
     4081 ttgctatagc gattctttgt ctttgccccc ctgacaactt agtacccctt tcccctacta
     4141 tagtattaaa accctgaggc aagttctgaa taaagtccat gcaatttgct aaagaagctg
     4201 ctgttactac ttcatccctg tcagcatcct ctttaccata ttgaatgttt tctaataagg
     4261 tacgatggaa tagattggta tcctgaggaa tcatactgat tgattttctt aaagaatatt
     4321 gcgtgatctc gctaatgtct tgattatcaa tcaatatcct tccgctatct acatcaaaat
     4381 aacggagaat tagattgata aatgttgttt tgcctccccc ggaatagcct acaagcccaa
     4441 ctttttgttt agccggaata accaacgatt tattggtaaa taattcctga ccatctctat
     4501 ggcgaaaggt cacattttca aacctgattt caccatgagt gactttcaac tcttttgcac
     4561 tggctatatc ttgaacttct ataggttgag ataatatagc aagcgcttgc ttgactatcc
     4621 ctatttcgac aaacaaatca caaagcgaat tacttagact ccacatttga ttcataatgg
     4681 caaatgatgt tgcgaatata aagaccacgt cacctgtgct gattaagttt ctctgccaga
     4741 aactgatgac tgaataaatc atggcaatta acataatact gacaggaaca tcgactaaaa
     4801 gacgaaaaaa attgataaaa agaaccaggc gcttattgct tttaacctct ttatcttgtg
     4861 ttttccaaac gtattttttt tcatactcat tttggacaaa taatttcacg gtattgatat
     4921 tattcaagga atcaacgatc tttcccttta aaatactttt atcttccgca ttcaccctgg
     4981 caagcttatt gatctcttta gctgaataaa agctgataaa taattggatt atgatccagc
     5041 ttaacaaaat gaatgcaaac cagggattaa tggttaacat taaaacgatt gaaactacaa
     5101 ttgccgcaaa agtagtgttt acattacaag atacgatttt acgaatggct tccaatgatc
     5161 gaggtatatc acttatctta ttagccaggt ttcccgctaa ttgattagaa aaaaaatgat
     5221 gggaatgctg gattaaataa ccgaataggg tcatgcggat attggcctga aattgaggaa
     5281 caaggtaggc ttgccaccaa ttatgcaagc gcaacaccag aatcacacca aaccaggcaa
     5341 ctccacccaa atacaaagcc ggtgcgatgt cttgaaatat gctttctttt ccccctttat
     5401 ggtttgttat cccatcaata atcattttta aagcataggg aatcacattc gtttcaagca
     5461 ccatcaccac tggtgctatg aaaaataaag cataaagcca gggacttttt tttataaaat
     5521 gccaggaaaa tctcaggata gtcttaggca attgttgtaa gtcagacata ttcacatcct
     5581 tgtaaaaaat ctggaacttc ttttttaaga tatcaaagtc aaagataaag agtaagtgtc
     5641 tataaccaat tagatgataa attgatgagt ttttgcgctt tgaattatca cttggtgaat
     5701 tttttgaata aagatatgcc tttttaaaat ttagggaata ctaagccata aaatagaaag
     5761 acagcatatt acacccaaaa accatacagt ggagcgcagg gaagaccaat tcagtaaata
     5821 aaacaaatgg taaaaaagcc gggaaataat ataggcgata gcgaaatatt gggtcattaa
     5881 agtgactgaa ttagtcgcca atgcagttaa tgaagctgtt gcaaatatca gtaaagactc
     5941 aaaagcattt tgatgcgctg ccagagctct tgcgccaaac ccttgcaaac gagcttgttg
     6001 ctctctggga taacgattat tatagccacc cgctttatgc atagcataag ctacaggaac
     6061 ttttgccaga taaggtagca atatagcgat aaacaagcaa ataattatag tatacatggt
     6121 tatagagtat ctcaggcata tatta
//
LOCUS       DAXLDT010000171         6132 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXLDT010000171 DAXLDT010000000
VERSION     DAXLDT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.171"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(18..1028)
                     /locus_tag="ACF2SP_002646"
     CDS             complement(18..1028)
                     /locus_tag="ACF2SP_002646"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360417.1"
                     /translation="MSTDNLLQPKLIVATLSDYPVIQNMARFYVYDMSRYCGFISEKW
                     ACPPDGLYESYDFKIYFTNPERKAFLIQIENELAGFALLNKFGTQAVDWNMGEFFILA
                     KFQGKGIGQSVAHQLWRTHPGEWEIPVIPENIPALSFWRQAVMTYTLNQYKEQTKTVN
                     YDIHQTQRIILSFNTQQQNKSCHSIPQKPITIDFVDSIPESLEKKMTEDLIAYERTHG
                     IDVNYKRFSIVLSAEGMPCGVINAFTAFAEIYIDDIWVDKTYRGKGYGRKLLQTLEDH
                     FKGQGFNNINLCTSAFQAPEFYKKCGYTAEFTRINQANPKLSKTFFVKFFDEEDQTQG
                     IF"
     gene            complement(1251..1865)
                     /locus_tag="ACF2SP_002647"
     CDS             complement(1251..1865)
                     /locus_tag="ACF2SP_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360418.1"
                     /translation="MVWDATITNAISNAAHAFFLLLYLIGACIHYFKKDHTFSLLIVF
                     FFLNLLVLKVLGVYVHYYPSHLHLPPAWIAISLLVIMLNYLLVQSMQMSDLCRVIVVF
                     LSIIFTYLFLTHDGNYTYIALPVILVYLIAAYYSQAKVRIGFVMVVISNLIWIVTRHI
                     ANYLTGHEIAIEYRYDNDIYHILLILSTYVIYRGIAEGQWKHPR"
     gene            complement(2022..2396)
                     /locus_tag="ACF2SP_002648"
     CDS             complement(2022..2396)
                     /locus_tag="ACF2SP_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2360419.1"
                     /translation="MLSDPNLVLFYVKNPAKSEEFYKNLLDTQPIESSPTFAMFIMKT
                     GLRLGLWAKEDIEPKAHQTGGGMELSFQVNSNEMVDEIHKQWSDKEISIIQAPTQMDF
                     GYTFVGIDPDEHRLRIFCLKRT"
     gene            2587..3345
                     /locus_tag="ACF2SP_002649"
     CDS             2587..3345
                     /locus_tag="ACF2SP_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360420.1"
                     /translation="MNSCVETFHQMERHFFSLISMGLLSLEGLQACNSGMPIPALNPV
                     FYSGKSTLLGAEIYQCKRFYESHSTPWALVVPDYLSDTEADKVLAQSGFLSIDQGVAM
                     YLSIGDLGISELVSDLLIRRMDNEMDTWILPTIPAFESTEEIAKIYRVRHQEAIDKSP
                     GIYHFSGFIQEQIVCSLTLTVMGDSARIDDVATFPKHQKKGYATQLILSVLQTLRDTN
                     ISWCFLEASADGLNIYKKIGFRELFKNLYYEAQC"
     gene            3347..4033
                     /locus_tag="ACF2SP_002650"
     CDS             3347..4033
                     /locus_tag="ACF2SP_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2360421.1"
                     /translation="MSKTERLFHLLQILRRYRYPLSGQYLANELGVSTRTIYRDIAVL
                     QAQGADIEGEPGLGYVLKPGFMLPPLMFNEEEIEALVLGSRWVAKKTDTHLQQAAHNA
                     LAKIASVLPADLRQQLELSSLLIGPGEVISGEDESLALVRKAIRKELKLFIQYHDLKG
                     NVSTRTIWPIGLGYFDQVRVLIAWCELKKDFRHFRADRIVDVQNLGVCYAKRRSVLLH
                     DWRKQNNIPG"
     gene            complement(4192..5451)
                     /gene="lem19"
                     /locus_tag="ACF2SP_002651"
     CDS             complement(4192..5451)
                     /gene="lem19"
                     /locus_tag="ACF2SP_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem19"
                     /protein_id="HFD2360422.1"
                     /translation="MIDMISITTYWQQFKDCIDQLRSSVDNKEYSNQIDIADLMVEIG
                     LTIEQLGITQIQQVGDRLHEKAKLFGYRVPFVTPEKKYEFCLPKITNAETLIFAIVYY
                     QHLKNYENVEYQGSINWFRSKVIHGVNEKKISTRAQAEAMLGEIRRYCQSQNIAFNES
                     EFIKKNDESAKEYTTRLFKNFHADKTAKSEPIGLKQLNESKESNLSDELAKLEKSLED
                     SRNKRNQLIVKIDALAKKLADYHLLSQNHHKLNDEWNNKWFFTKFFDWIMSWFSNDSF
                     IKNLKTSYEQVINAEHELKQEFHPFDTTDAYRAELKKQLEETHIECDKNQKQINEYQL
                     KQLEHKLEQRLEQKLQEKLQKDTAVEQKEPSVTKVLSEIKHETNGSDFSSNLNHYYGF
                     FKEHLPNRETLGAIAVGVAAIAIQNLM"
     gene            complement(5586..6098)
                     /locus_tag="ACF2SP_002652"
     CDS             complement(5586..6098)
                     /locus_tag="ACF2SP_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360423.1"
                     /translation="MSVINGKKIITIILGIACLFCSIQWVNSAPDFSNKPHVREKNKN
                     LLGVFVGGNHAQNDEAFTYGFEYHRVLSMPFGFSAVIEHTPVNVEHNNQVELIGLGTL
                     NVFRNLTFGIGPGIRYEKDEPNRMVGRLGIGYIIHFPPDIEITPNIDLDFIEGGEKEL
                     IFGITFGKQF"
BASE COUNT         1829 a         1069 c         1171 g         2063 t
ORIGIN      
        1 ctataaatcc tgttttttca aaatattcct tgtgtttgat cttcttcatc aaaaaatttc
       61 acaaagaagg ttttggaaag cttgggattg gcttgattga tgcgtgtaaa ttcagccgta
      121 tatccacatt ttttgtaaaa ttctggtgct tgaaaggcgc tggtacataa attgatatta
      181 ttaaatcctt gccctttaaa atgatcttca agagtttgca gtagttttct gccatatcct
      241 ttaccacgat atgttttatc tacccagata tcatctatgt atatttccgc aaaggcagta
      301 aaggcattaa taacgccaca aggcatccct tctgcagata gtactattga aaaacgcttg
      361 tagttcacat caatgccatg agttcgttca taggctatca gatcttctgt catctttttt
      421 tccagagatt cagggatact atccacgaaa tctattgtaa taggtttttg gggaatagaa
      481 tgacaagatt tgttctgctg ttgtgtatta aaacttaaaa taatgcgttg ggtttggtga
      541 atatcataat tcacagtctt tgtctgctct ttatactgat taagggtata ggtcattacc
      601 gcttgacgcc aaaacgataa agccggtatg ttttctggaa taaccggtat ttcccattca
      661 cctggatgag tacgccataa ttgatgggct actgattggc caataccttt cccttggaat
      721 ttagccagga tgaaaaattc ccccatattc caatcaacag cttgggttcc aaatttattt
      781 aataaagcaa aaccagccag ttcattttca atttgaataa ggaaggcttt tcgttcgggg
      841 ttagtaaaat aaattttgaa atcataactt tcataaagtc catctggtgg acatgcccat
      901 ttttccgaga taaaaccgca ataacgtgac atatcataaa cataaaatcg tgccatattt
      961 tgaataactg gatagtcact aagtgttgct acaattaact tgggctgtaa taaattatca
     1021 gtgctcatga atatctatac tggagtgagt ttcttaacta tacggataag gtatcaaagt
     1081 gtgctgtggt tttatagagc gattgttgtt atttgcataa ttaatctacc aaaagatttc
     1141 catgaatgta tgaattcatg ctcaagaaat gtctcatttc aaagtggtta aattattcac
     1201 tcataccctt aatattgatc ttgataatta tagttttctc aataatattt ttagcgagga
     1261 tgtttccatt gcccttcggc aataccccta tagatcacat aagtagagag aattaaaagg
     1321 atatgataga tatcattatc atagcgatat tctatagcta tttcatgacc tgtgagatag
     1381 tttgcaatat gtcttgtaac tatccatatc aggtttgata taacgaccat tacaaagcca
     1441 atcctgactt ttgcttgcga ataataggcc gcaatcaaat atacaaggat gacaggaagg
     1501 gcaatgtagg tataatttcc atcgtgggtt aggaacagat aagtaaatat gatcgataga
     1561 aatactacga taactcggca caaatcggac atttgcattg attgaacaag cagataattc
     1621 agcatgatta caagcaaact aatcgcaatc caggcgggtg gtaaatgcaa atgagaaggg
     1681 tagtaatgga cataaactcc caataccttg agaacaagta ggtttaagaa aaaaaataca
     1741 atcaaaagtg aaaaagtatg atctttttta aagtagtgaa tgcaagcacc aattaagtac
     1801 aataatagga aaaacgcatg agccgcatta gagatggcgt ttgttattgt tgcatcccat
     1861 accattctgt atctcccttt aaaaatacag ctgtcagggt tcgaggtcct gctgtttgtt
     1921 aatttactaa tagtaattgt agcttgtagc aggttaagat gtgtgatatc agtcacttaa
     1981 aagccatact acaagctatg attaatcatt actccaattg tttatgttct ctttagacaa
     2041 aaaatacgaa ggcgatgttc atcgggatca ataccaacaa acgtgtaccc aaaatccatt
     2101 tgtgtcggag cttgaataat agaaatttct ttgtcagacc attgcttatg tatctcatcg
     2161 accatttcat tactattgac ctgaaaggac agctccatac cccctcctgt ttgatgagct
     2221 ttaggctcaa tatcttcttt cgcccataat ccaagacgaa gccctgtttt catgataaac
     2281 atagcgaatg ttggtgaaga ttcaataggt tgtgtgtcta acagattttt ataaaattct
     2341 tcactttttg ccgggttttt aacgtaaaat aaaaccaaat tagggtcgct taacatgtta
     2401 ttttccttat ttttttatta atcatcacca gtataaacaa ggctaatgac agattttgtc
     2461 agtagtgttt taagtgccta atattgatct tattgagaat aaaaaacaca cgaaacagga
     2521 ttttggtgtt attatgaaaa caaaatttag cctatcctgg ctcaaaaatt ttgatgagtt
     2581 tattttatga acagctgtgt tgaaactttc catcaaatgg agcggcattt tttttcactg
     2641 attagtatgg ggcttttatc attggaagga ttgcaagctt gtaatagtgg aatgccaatt
     2701 ccggctctaa atcccgtgtt ttattcaggt aaatcgactc tgcttggggc agagatttat
     2761 caatgcaaac gcttttatga gtctcattca acaccctggg cactggtggt tcctgactat
     2821 ttatcggata cagaagcaga taaggtactc gctcaatcag gattcttatc tattgatcaa
     2881 ggcgttgcca tgtatttgtc aattggtgat cttggaattt ctgagcttgt atctgacttg
     2941 ttgataaggc gcatggataa tgagatggat acctggattt tacccactat ccctgcattt
     3001 gaatcaacgg aagagattgc aaagatttac agggtgaggc atcaagaggc tattgataaa
     3061 tcccctggga tttatcattt ttccgggttt attcaagagc aaattgtctg ctcattaact
     3121 ctgactgtta tgggggacag tgcgcgaatt gatgacgtag ccacttttcc caaacatcag
     3181 aaaaaagggt atgcaacaca attaatttta tctgtacttc agactctcag agacaccaat
     3241 atttcctggt gctttttaga ggcatctgct gatggtttga atatttataa aaagattggt
     3301 tttcgtgaac tgtttaaaaa tctttattat gaagcgcaat gctaatatga gcaaaactga
     3361 gagactcttt catctgttgc aaattttgcg gcgttaccgt tatcctctta gcgggcaata
     3421 tcttgccaat gagctgggtg taagcacccg taccatctat cgggacattg cggttttaca
     3481 ggctcaaggt gctgatatag agggtgaacc aggattgggt tatgtgttaa agccaggttt
     3541 tatgttaccg cctttgatgt ttaatgaaga ggaaattgag gctcttgttt taggctccag
     3601 atgggtggct aaaaaaacgg atactcactt gcaacaggcc gcacataatg ccttggctaa
     3661 aatcgcttct gtacttcctg ccgatttgcg gcagcaattg gagctatcca gtttattaat
     3721 agggcctggt gaagtgattt caggagaaga tgaatccctg gccttagtca ggaaggcaat
     3781 acgaaaagaa ctgaaattgt ttattcaata ccatgactta aaaggaaatg tttcaacacg
     3841 tacgatttgg ccaattggac ttgggtattt tgatcaagta agagttttga ttgcctggtg
     3901 tgaattaaaa aaggattttc gtcattttag agccgataga attgtagatg ttcaaaacct
     3961 gggagtatgc tatgcaaagc gacgctcggt gttacttcat gattggcgaa agcaaaataa
     4021 tatcccaggc tgatgacaac acgatgttat tgaggtgtta acttgaacag gcaaaattta
     4081 atgccgggaa tcaagtttta ctttcttccc ggattaagat tgcttcttat gcgaaagacg
     4141 agctgaactg ccattattcc tgagtgagag agcctcgtat ttcaaacggc tttacatcaa
     4201 attttgaatg gcaatggctg cgacaccaac agcaattgct cccaatgttt ctcgatttgg
     4261 caagtgctct ttaaagaaac cataataatg atttaaattt gaagagaaat cgctgccatt
     4321 ggtttcatgt ttgatttctg atagcacttt tgtaacggaa ggctcttttt gttcaacagc
     4381 agtgtccttt tgtagttttt cttgcagttt ttgttctaac ctttgttcaa gtttatgttc
     4441 taactgtttc aattgatatt cgttgatttg cttttgattc ttatcgcatt caatatgagt
     4501 ttcttccagt tgttttttta attcagctcg ataggcatcc gtggtgtcga aggggtgaaa
     4561 ttcttgcttc agctcatgtt cagcattaat gacttgctca tagctggttt ttaaattttt
     4621 gataaatgaa tcattagaaa accaggacat gatccagtcg aaaaattttg taaaaaacca
     4681 tttgttattc cattcatcgt ttaatttatg gtggttttgc gaaaggagat gatagtctgc
     4741 cagtttcttt gcaagagcat ctattttgac aatcaattga tttcttttat ttcttgaatc
     4801 ttcaagggat ttttcaagct tggctagttc atcggacagg ttggattctt ttgattcatt
     4861 aagttgtttt agcccgattg gttcactttt tgcagtttta tcggcatgaa aattcttgaa
     4921 caatcttgtt gtatactctt ttgctgactc gtcatttttc tttatgaatt cagattcatt
     4981 aaaggcaata ttttgacttt gacaatatcg tctaatttct cccaacatcg cttcggcttg
     5041 agcacgggtc gaaattttct tttcattaac accatggatt acctttgatc taaaccaatt
     5101 gatagaacct tggtattcga cattttcata gttcttaagg tgctgataat aaacgattgc
     5161 aaaaatcaga gtctcagcat tagttatttt gggtaaacaa aattcatatt ttttttcagg
     5221 ggtaacaaat gggactcgat atccgaacaa tttggctttt tcgtgcagtc gatctcctac
     5281 ctgttggatt tgagtaatac cgagttgttc tattgtcaag ccaatctcga ccataaggtc
     5341 cgcgatgtct atttgattgg agtattcttt gttgtctaca gatgaacgaa gttgatcaat
     5401 gcaatcttta aattgttgcc aataagttgt tattgaaatc atatcaatca ttatttaaga
     5461 aaaaagagtc ataaatttac accggtcttc taaaaaaagc tattatttag tcgtgattat
     5521 caatcagaaa atttttaaaa agtgagaaag caggagagac aattcatata ggggaaaaga
     5581 gctatttaaa attgtttgcc aaaagtaata ccaaagatca gctctttttc acccccttca
     5641 ataaaatcaa gatctatatt cggggtgatt tctatatccg gaggaaaatg tatgatatat
     5701 ccaatcccca atcgaccaac catgcggttg ggttcatctt tttcatatct aattccaggg
     5761 cctatgccaa aagtcaggtt cctgaatacg tttaatgtac caaggcctat cagttctact
     5821 tgattattat gttccacatt gacgggtgta tgttcgataa ctgcactaaa tccaaaaggc
     5881 atggaaagga ctcggtggta ctcaaatcca taggtaaatg cttcatcgtt ttgagcatga
     5941 tttccaccca caaaaacacc aaggagattt ttgttttttt ccctcacgtg cggtttgttt
     6001 gagaagtctg gagccgaatt tacccattgt attgagcaaa aaaggcatgc aattcccaga
     6061 atgatggtta ttattttctt tccatttatt accgacatga attctcaata cagttttatc
     6121 tatttcttta tt
//
LOCUS       DAXLDT010000172         6127 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXLDT010000172 DAXLDT010000000
VERSION     DAXLDT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.172"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            225..1370
                     /locus_tag="ACF2SP_002653"
     CDS             225..1370
                     /locus_tag="ACF2SP_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HFD2360424.1"
                     /translation="MLRWIVLICISLFLPLSAYADKSDCINHHCIAVIDAGSSSSRLH
                     IYAYDLDETNTPVAIREIWNRKAIPGLSTIEHENKSVSAYMEKLFSDAPAVLMPVYFY
                     STAGMRLLPKTHHRNVNQLVAEWFDRQYYWRLVTARTISGTEEGIFAWLATNYQLNLF
                     QQEPQTLAGVMDIGGASVQIVIPVVNPSLVDHEHTTTIRLYGKEFTVFSHSFLGLGQN
                     EMGHQFFDLKFCFPEEYELPSGRQARGDAYACKDEIAILVNSVHSVSRKIKSVLAANP
                     IEKWYILGGITFMLNDKLFSLDNQYFTNRDLISFADQSICHQKWSTLEELYPDNLMVF
                     NYCMLPSYLYALLVHGYGLKSYQKVNFMPNSKNIDWTLGVVLQHKQS"
     gene            1387..1459
                     /locus_tag="ACF2SP_002654"
     tRNA            1387..1459
                     /locus_tag="ACF2SP_002654"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Thr,seq:cgt)
     gene            1625..3430
                     /locus_tag="ACF2SP_002655"
     CDS             1625..3430
                     /locus_tag="ACF2SP_002655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360425.1"
                     /translation="MGIFLETKRLILKRVELSDLDLLVALRSDLEVMENTGYGGTQTI
                     EEVSEYLDFAIPYQEKHGMGFCLVFEKESRNFVGEAGLFHLLFDDKQSEIELGYHLHK
                     KFWGKGYGTELAKALIQWGFQHLSVNKLISTTYPDNIASQKVLNKAGFDCMSKKQLPD
                     GEELFWYEIYKNDSVELVPYDSQWPKMAEVEIKKLRKILPTNHIIDIQHVGSTAIPGM
                     LAKPIIDIQVTVDSLTAIKQTAIDVLKAYDYVYWAEDPDPEKMFFVKGMPPFGEKRTH
                     HVHIIEPKSKRGQDRIIFRDYLIAHPEVAREYEQLKIELAHEYTYDRERYTDAKTQFI
                     ASVLGKALVTDQIQNPTKYKGINELLHQLLLQIQSILANEFVGMYIGGSLASDSFNSE
                     TSDIDCYIITSKMLSENMIGKIEEMHNQFYSSKLKYAKKIEASYVPQKDLLDFDPQGI
                     RPYFNEGRYYLGHYGNNYLIELFVLREKGISIAGPDIKCLIQEISTQSLKAAIQKNLH
                     EYWESNLIDFSKFKRSDYQVFTILTMCRTLYSLETGKITSKIEAAQWAMQQLDTKWKK
                     LIELAAAWEPSQEINRLEETQNFVRYVLNKSREYQ"
     gene            3543..4148
                     /locus_tag="ACF2SP_002656"
     CDS             3543..4148
                     /locus_tag="ACF2SP_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFD2360426.1"
                     /translation="MSKLNKTRVYEVYDEIINWFDDARTKNLMESEYLNLIVNAIPPK
                     SSILDLGCGTGEPIAQFFIEKGFKVTGVDGSQKMIELCRKRFPDERWIVSDMRDINLQ
                     QQFDAILAWHSFFHLDHDSQRNMFKIFESHTKPGGVLAFTSGEEEGEVWSDNGGQQLY
                     HASLSTKEYESLLKNSSFKVLVHKVCDPECGEATVWVAQKI"
     gene            4480..5589
                     /locus_tag="ACF2SP_002657"
     CDS             4480..5589
                     /locus_tag="ACF2SP_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFD2360427.1"
                     /translation="MKWNWQLESWPNFTWDSDKLVVYEQSFTENAGIIIGSSQHISQE
                     GKQNLFIDLMCTDALDSSEIEGEHLNRDSIQSSIKKELGLSAEAPRASLAERGIAKMM
                     VNLYQTISSPLTHQVLFEWHQFLMGNSHHLEDAGQYRSHEEAMQIVSGPDYDRKTHFE
                     APPSKCVPAEMEQFINWFERSSPAGSASLPTLTRAGIAHLWFESIHPFEDGNGRIGRA
                     IAEKALSQGFSKPVMTVLAKILLKKRKEYYQQLGLASKTLDLTSWLLWFANIALEAQQ
                     STYMYIDFIIKKSVILREAEGKINPRQEKVLLRLFHAGPEGFVGGLSAKNYMSITGAT
                     IATTTRDLNDLVKKNILKRTGELKATRYFLNLDKA"
     gene            5673..>6127
                     /locus_tag="ACF2SP_002658"
     CDS             5673..>6127
                     /locus_tag="ACF2SP_002658"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360428.1"
                     /translation="MLQFMFFDDTYLDYKARVSYSLFCAKDFNETAAFIKANRILMTG
                     TELAAYMLKPENYKYAIIARIKNNIVGIVLFTDFESEVFINHLCVSPIHRFKGIGSNL
                     LKELKAFMGQITVSYIDTSNNYMPWMEVLDVSKKAAVINQLGSFYKRSR"
BASE COUNT         2000 a          996 c         1203 g         1928 t
ORIGIN      
        1 atgaaaaatt tcgcgtttat agcataaatt ttgaactttt aaaagaaaca atttcattaa
       61 tttttatttt tttatctttc ttcattttga tggaaaaatg agtttcacat tgatatggat
      121 ttccaggtgt ctcgctttta ttcgcaaatt cagttcctgt tttgaccaaa aaaaacaatt
      181 aatgtaggat aaatgcgtta cttgaggtga attaaggaaa gactatgttg cgatggatcg
      241 tgttaatttg tatctctttg tttctgcctt tatctgctta tgcggacaaa tccgattgta
      301 tcaatcatca ttgcattgcg gtaattgatg ctggaagttc cagttcacga ttacatattt
      361 atgcctatga tttggatgaa acaaacacac ccgttgcaat tagagagatt tggaaccgaa
      421 aagccattcc cggattgtct acaattgagc atgaaaacaa atcggtttca gcctacatgg
      481 agaagctatt ttcagatgct ccagctgttt taatgccagt ttatttttac tccacagcag
      541 gcatgagatt gttacctaaa actcaccaca gaaacgtcaa tcaattggta gctgagtggt
      601 ttgacaggca atattactgg cgacttgtga ctgcaagaac gatttctggt acagaggaag
      661 gtatttttgc ctggctcgca accaattatc aactaaacct gtttcaacaa gagccccaaa
      721 ctttagctgg tgtgatggat attggcggag catctgttca aattgttatt cctgttgtaa
      781 atccctcttt ggttgatcat gagcatacaa ccacaattcg cctctacggc aaggaattta
      841 cggttttctc tcatagtttt cttggtcttg gtcagaatga aatgggccat cagttttttg
      901 atttaaaatt ctgctttcca gaggaatacg aactgccaag cgggcgccaa gctcgaggcg
      961 atgcctatgc ttgcaaagat gaaattgcca ttttagtcaa tagcgttcat tctgtgagcc
     1021 gaaagattaa atcggtatta gccgctaatc ctattgaaaa atggtatatt ttgggcggca
     1081 ttacctttat gctgaatgac aaactatttt ctctggacaa tcaatatttt accaatcgtg
     1141 atcttatttc ctttgcagat caaagtattt gccatcaaaa atggtcaact cttgaagagc
     1201 tctatccgga taacctcatg gtgttcaact actgcatgct accttcttac ctttatgcct
     1261 tgttagttca cggctatgga cttaagagtt accagaaagt taatttcatg cccaatagca
     1321 agaatatcga ttggaccttg ggagtggtat tgcaacataa gcaatcttaa gtatactttt
     1381 ctttaagccg ttgtagctca gttggtagag caattgattc gtaatcaata ggtccgcggt
     1441 tcgattccgc gcaacggcat aaaaaatcaa taattatcat aaagatacag aagttatctc
     1501 tgatttaaaa ttaaatacaa tattttcgtg ctaccccggt actacctttc gtagctactg
     1561 ctatcgtaga agcatcaaat aaaaagtata attctttcca gtttaaaata tagatagaca
     1621 cccaatgggc atttttctag aaactaaaag actaatactt aaaagagttg aattatccga
     1681 tcttgatctg ctagtagcat tgagatctga tttagaagta atggaaaata ctggttatgg
     1741 gggtactcag acaatagaag aggtaagtga gtacttagat tttgctattc cttatcaaga
     1801 aaagcatgga atgggatttt gtctggtatt tgaaaaagaa agcagaaact ttgttggcga
     1861 agcgggttta ttccatttgt tatttgatga taagcaatcc gaaattgaac ttggttacca
     1921 cttgcataaa aagttttggg ggaaaggcta tggcactgaa ttagcaaagg cactaattca
     1981 gtgggggttt cagcacttgt ctgtcaacaa gctcatttct actacctatc cagataatat
     2041 cgcttctcaa aaagtcttaa acaaagcggg ctttgattgt atgagtaaaa aacaattgcc
     2101 tgatggcgag gaattgtttt ggtatgaaat ttacaagaat gattccgttg aattggtgcc
     2161 ttatgattct caatggccaa agatggctga agtggaaatt aaaaagctac gaaaaatcct
     2221 gccaactaat catattattg atatccaaca tgttggtagc acagctattc caggaatgct
     2281 ggctaagcct attattgata ttcaagttac tgttgattct ctaactgcta taaaacagac
     2341 agcgatagac gtattaaaag cctatgacta cgtatattgg gccgaagatc ctgatcctga
     2401 aaaaatgttt tttgtaaaag ggatgccacc atttggtgaa aaacgaacac accatgtgca
     2461 tattattgag cctaaatcaa agcgtgggca agatagaatc atttttaggg attatttgat
     2521 tgctcatcct gaggtagctc gtgagtatga acaattaaaa atagaattgg cacatgaata
     2581 tacttatgat cgggagcggt ataccgatgc taaaacacaa ttcattgcta gtgtattagg
     2641 caaagctcta gtaactgatc aaattcagaa tccgacaaaa tataaaggaa taaatgaact
     2701 actacaccag ctcctcttac aaatacaatc tattctagca aacgaatttg taggtatgta
     2761 cattggaggt tctctcgcaa gtgattcatt caatagcgaa acaagtgata ttgattgcta
     2821 cattattaca agcaagatgc tttctgagaa tatgatcgga aaaattgagg aaatgcacaa
     2881 tcaattttat tcaagtaaat taaaatatgc gaaaaagatt gaagcttctt acgttcctca
     2941 aaaagatctc ttagattttg atccccaagg catacggcct tattttaatg aaggtcgcta
     3001 ttacctgggg cattatggca ataattattt aatcgaacta tttgtactaa gagaaaaagg
     3061 aatttcaata gcagggcccg atataaaatg tttaattcaa gaaatatcca ctcaaagttt
     3121 gaaggctgct atacagaaaa atttgcacga atattgggag tctaacttaa ttgatttttc
     3181 aaagtttaaa cgcagtgact atcaggtgtt tacaatttta accatgtgcc gcacccttta
     3241 ttctttagaa acaggcaaaa taacttctaa aatagaagca gcacaatggg caatgcagca
     3301 gcttgatact aaatggaaaa agttgattga attagctgcc gcttgggagc caagccagga
     3361 aataaataga ttagaggaaa cgcagaattt tgtcaggtat gtgctgaata aaagtcgcga
     3421 gtatcaatga gcagatcaca ttacttttca gaataaatga agactgaaag ttttttgtag
     3481 tagtctgata aaatctcacc catcaataaa tccagctatt gttattttag gagcaattga
     3541 gcatgagtaa gttaaataaa actagagtct atgaagtata tgatgaaatc attaattggt
     3601 ttgatgatgc gcgcactaaa aacctcatgg aatcggaata tcttaattta attgtgaatg
     3661 caattcctcc gaagagctca attttggact tgggctgtgg aactggagag cctatcgcgc
     3721 aattttttat tgaaaaaggc tttaaagtta ctggtgttga tggcagtcaa aaaatgattg
     3781 agctttgtag aaagcgtttt ccagatgaac gctggatagt ctcagatatg cgcgatataa
     3841 atttacaaca acaattcgat gcaatacttg catggcatag cttttttcat ctggatcatg
     3901 acagccaacg taatatgttc aaaatatttg aatcccatac caaacctgga ggagtactag
     3961 catttacttc tggagaagaa gaaggcgaag tctggagtga taatggaggc caacaacttt
     4021 atcatgcatc tttgtcgaca aaagaatatg aaagccttct caaaaattca tcatttaaag
     4081 tgctggtaca taaagtctgt gatcctgagt gtggcgaggc tacagtttgg gtagcgcaaa
     4141 agatttaaaa tccttcgtta taaaccaaaa ttatcagtgg aattacctat gaaccctaat
     4201 tttaaaataa gatttgcaat actgtctgat gttcctttaa ttttgcaatt tattaaagtg
     4261 ttagcagaat acgagtatca gcgctaaagg gtcagatcct gatgaatcct gaatcaaatt
     4321 aatgcttaaa atggtagtct ccacattaca aactagcgca aaaagccttt ttaattaatt
     4381 cactaaacat ataaataaat taatgctgat taataaaacc atgatttatt aatcactaat
     4441 atgctatatt ttgattaata aaatatgagg ttattaatca tgaaatggaa ttggcagctt
     4501 gagagttggc ctaactttac ttgggactcc gataaactag ttgtgtatga acagtctttt
     4561 actgagaacg cgggcattat tattggttct tcgcaacata tatctcaaga aggtaaacaa
     4621 aatttattta ttgatttaat gtgtactgat gctttggata gttctgaaat tgaaggagag
     4681 catttaaaca gagacagcat tcaatcttct ataaaaaaag agttgggctt gtctgcagaa
     4741 gctcctagag ccagcttggc ggagcgtggg atcgctaaaa tgatggttaa tttatatcaa
     4801 acgatttcta gcccactaac acatcaggtt ttatttgaat ggcatcaatt tttaatgggt
     4861 aatagccatc atttggaaga tgctggtcaa tatcgtagtc atgaagaagc aatgcaaatt
     4921 gtttctggac ctgattatga tcgaaaaacc cattttgaag cgcctccttc aaaatgtgtt
     4981 ccagctgaaa tggagcaatt tattaattgg tttgaaagga gttctccagc cggctcagcg
     5041 tctttaccca cattaacccg agcaggaata gctcatttat ggtttgagag tattcatcct
     5101 tttgaagatg ggaatggaag aatagggcgg gctatagcag aaaaagcctt gtctcaaggt
     5161 ttttcaaaac ctgtcatgac ggtattggca aaaatattat taaaaaagag aaaggaatat
     5221 tatcagcaat taggtttagc aagtaaaacc ttagacctga catcctggct tttatggttt
     5281 gctaacattg ctctggaggc gcaacaaagc acttatatgt atattgattt tattattaag
     5341 aaatctgtta ttttaaggga agccgaaggg aaaattaatc caaggcaaga aaaggtttta
     5401 ttaagattat ttcatgctgg gcctgaggga tttgtcggtg gtttaagtgc taaaaattat
     5461 atgagcatta ctggtgcaac gatcgctaca acaaccagag atttaaatga cttggttaaa
     5521 aaaaatattc tcaaaagaac tggtgagctt aaagcgacgc gttatttttt aaatcttgat
     5581 aaggcttaaa tggagtttgt gagatttggt acgcagtgtg tactgtacat ttggcaggtt
     5641 ttgtatttaa atgcatgcca tctagatcgt taatgttgca atttatgttt tttgatgaca
     5701 cttatttgga ttataaagca agagttagtt actctttgtt ttgcgctaaa gattttaatg
     5761 aaacagctgc ttttatcaaa gctaatcgaa ttttaatgac tggcaccgag ttagccgctt
     5821 atatgctaaa accagaaaat tataaatatg ccattattgc cagaataaag aataacattg
     5881 tgggcattgt cttattcacg gattttgaat cggaagtatt cattaatcac ctctgtgtaa
     5941 gccctataca tcgttttaaa gggatcggct caaatttatt gaaagaatta aaagctttta
     6001 tgggacaaat aacagtctct tatatagata ctagtaataa ctatatgcct tggatggagg
     6061 ttttggatgt ttccaagaaa gctgcagtta ttaatcaatt aggttcattt tataaacgta
     6121 gtaggtg
//
LOCUS       DAXLDT010000173         6036 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXLDT010000173 DAXLDT010000000
VERSION     DAXLDT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.173"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..204
                     /locus_tag="ACF2SP_002659"
     CDS             <1..204
                     /locus_tag="ACF2SP_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2360429.1"
                     /translation="IFCKETIAEYLEGRCKNWFFHPFKFNLELNQREDVIRTWTMKTE
                     FNDKQLTNGMKLLVDHYLLEIHI"
     gene            217..1308
                     /locus_tag="ACF2SP_002660"
     CDS             217..1308
                     /locus_tag="ACF2SP_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFD2360430.1"
                     /translation="MSQPFTIGPFSVEENHPPIFLAEIGTFFNQDLGIAEQLLHKIIT
                     IRDKVPYQPLILKTEILNNPNICLPVDYEETYTSKSGEVRKENYRSLIERKVLPLEHY
                     SMLFSLLKNANLPFIVSIYELDTIDFAIKQGACALKTSSSNIVHVPLIRHLAKTGLPL
                     IIDTGRSSLTDICKAVETAKSAGCEKIIIEHSPDGHPALPEAHNLRMLRTLNQCFSLP
                     VGLSDHYNGIEMLYMSIALGASILEKGVHLYPEDIDQDISHTMGIEQLAKTLETIYNC
                     WQALGQPMRDLEAPIKGVLGSSQRTCLVAKQKLQPGSLISLDNTTFAFPCKGVPTQYW
                     DLVNGWELQNKVSIGEPIKWSDIKQKNDC"
     gene            1262..2299
                     /locus_tag="ACF2SP_002661"
     CDS             1262..2299
                     /locus_tag="ACF2SP_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2360431.1"
                     /translation="MNPLNGRILSKKMIVRNPTWNSLTPPLPKFVSCDTVETNFLKEK
                     LGEDGGIYTAQFDLKSTYAFLFQRKNDQKKFFIKKVSAEHKRQYQQSEDLAQFIACPD
                     YIVNIAINCISNEEENSLYYVYPYIHGKRLFAEPEQIITLATALAKLHLKKKSYPDQQ
                     LIIKNTTERTSQLNFIRKALADGCYSYIPHFSFVKKMAQQYDFDWINQEDAQPIHGDL
                     NAGNLLLSEDNMICFFDFEDALHSFHPVVLDLLFVIERIIFNQNSSTEQLLNLGLMFI
                     HAYKEAGGTYQYKTRDEFGLTILALKAFCLLTLLAEKNKNILNSEWDKFFKLTQKAGN
                     ERDLIKTILQG"
     gene            2310..3428
                     /locus_tag="ACF2SP_002662"
     CDS             2310..3428
                     /locus_tag="ACF2SP_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360432.1"
                     /translation="MKILVTARDVGAALNIIEIVKILKQYAGVTVFIYAQPPASKYFL
                     RAGIQPVFEVPLPPSRSLNEASDLLSYANQLIDKLNPDIILSGLSSPGEAGIDEAIIA
                     ECPAHIKTFVLQDFWGEVNFFFEKLADCYLCIDHHAAEITKKRFNAKTEVIGSPRHAI
                     YQTLNIEEQRNYIREYFNITQSTKTIGLFGQALHGIPGYMETISQWLEACLQQEKTKL
                     VILYRPHPRESEADKEETISLLISSGLEWKILSTQLVEESLLACDLVCSFFSNCLYDA
                     AYLNYFSLRPILTPVVLNFHPQIKNYTNQLSLYEISPYRKQELAFFCEYPHTLAKDID
                     YLLSSECSHKYWLHSKVKLEHPTKSLTRELAILLTGEP"
     gene            3431..5227
                     /locus_tag="ACF2SP_002663"
     CDS             3431..5227
                     /locus_tag="ACF2SP_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360433.1"
                     /translation="MPSIISDNPKNWSEEIKNYLCRSSPERYEKMATNNAIEPYISKA
                     ISWGYAEWKNRKTLLDDIETEMLRLATLPDLSDFNDNEIYSSKKNYLSKEILENYLIK
                     ASPENFNTVFRQPGWKSHVNFLIEKGNRELERQIINGNGVENSVDTVCAELAKLMNDF
                     ENSFFDEIYGNSTPRYLLPNLFEKAIAEYKLLATIKERTRFIEAFIEIVMFDWQFSGT
                     LDSDLFKNAYSKEVLYYYGIGARLFVLGEFKEAQEYFKKIINIEPKPIDYLMLSKTYR
                     ALNEKVETFNTIKDGLTHFPNDFLLTLALSGTLYWMGETKLANETLEPVKKDFNTMYK
                     SEIEGAKKLAVEISEAIKCDKIYRDQKNDIYDDKYTKKAWYQYWYHYNCFRPTQHPEA
                     YMIDYIPRYLTSIAKDATITLKGVLDFGTLCAEPLYRFAKNNPEIQCVGIDRQDLVKE
                     LNDKAYSLNNLRFEAGDIFEYLEHFALLVPNHRMLFHCRTAVLCYPNFLLKLYKKCAE
                     LGIEYICLMESNSLSRDTLTFYNYGEMPNISIARRGSLINHDYFLLLEQSGYEIVKKD
                     FHLAPHLLEDYAALIGSNTTLVAQLKLLTQSQ"
     gene            complement(5328..5624)
                     /locus_tag="ACF2SP_002664"
     CDS             complement(5328..5624)
                     /locus_tag="ACF2SP_002664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360434.1"
                     /translation="MQGDGTCPDDIPYAESRYASNPGALSAYSVVIAWGEGGATTLVE
                     TFAWFIVAAHYKHELAKIVEGHRGYPDDVVAQAEIMAKKILSSTKLTLYSEIMS"
     gene            complement(5801..>6036)
                     /locus_tag="ACF2SP_002665"
     CDS             complement(5801..>6036)
                     /locus_tag="ACF2SP_002665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFD2360435.1"
                     /translation="TYSHAFATGIYTVGNLCSIERNLIKLGNATRNGENVGIALYMGS
                     GCRISNNDIVFDKWLTWGRNFGIWTKLEKENTL"
BASE COUNT         2079 a         1075 c          986 g         1896 t
ORIGIN      
        1 attttttgta aagaaacgat agcagaatat cttgagggtc gttgtaaaaa ttggtttttc
       61 catcctttca aatttaatct tgaattgaat cagcgtgaag acgtaattag aacctggaca
      121 atgaaaaccg agtttaatga taagcaatta actaatggta tgaaattact ggtagatcat
      181 tatttattag aaattcacat ataagcgaga tagtcaatga gtcagccttt tacaattggt
      241 ccattctctg ttgaagaaaa tcatcctcct atttttttag cagaaatagg tacttttttt
      301 aatcaggatc ttggcatagc tgaacaattg ctacacaaaa ttataaccat cagggataaa
      361 gtaccatacc aacctcttat actaaaaaca gaaattttaa ataacccaaa catttgccta
      421 cctgttgatt atgaagaaac ctataccagt aaatccggtg aggtccgcaa ggaaaactac
      481 cgctcattaa ttgagagaaa agtactgcct ttagaacatt atagtatgct attttcacta
      541 ttaaagaatg ccaatttacc cttcatagtt tcgatttatg aattggacac tattgatttt
      601 gcgataaagc agggtgcctg cgcactcaaa acctcttcat cgaacattgt ccatgttcct
      661 ttaattcgtc atctggcaaa aacaggatta ccgctcatta ttgacactgg aagatcttct
      721 ttaactgata tatgcaaagc agttgaaaca gcaaaaagtg ccggatgtga aaaaattatt
      781 attgaacata gtcctgatgg acatcctgct ttgccagaag cacataacct aagaatgtta
      841 cgaactttaa accaatgttt ttcattacca gttggtttat ctgaccatta taatggaatt
      901 gaaatgttgt atatgtctat tgcattggga gcttcaatcc tggaaaaagg agttcatctc
      961 tatccagaag atattgatca ggacatatca catactatgg ggattgagca attagccaag
     1021 actcttgaaa caatttacaa ttgttggcaa gctttaggtc aaccgatgcg agatctagaa
     1081 gctcctataa aaggagtact tggcagctct caaagaactt gtttggttgc aaagcaaaaa
     1141 cttcaacctg gctctctaat tagccttgat aatacaacct ttgcctttcc ttgtaaggga
     1201 gtcccgaccc aatactggga tctggtaaat ggctgggaat tacaaaacaa ggtatctata
     1261 ggtgaaccca ttaaatggtc ggatattaag caaaaaaatg attgttagaa accctacttg
     1321 gaactctctt acccccccac ttcctaaatt tgtttcttgt gatacagtcg agacaaattt
     1381 cctgaaagaa aaattaggag aagacggcgg catttatact gctcaatttg atctaaaatc
     1441 aacctatgct tttttgtttc agcgtaaaaa tgatcaaaaa aaatttttta tcaagaaagt
     1501 atccgctgag cataaaaggc aatatcagca gagcgaagat ttggctcaat ttatcgcttg
     1561 cccagattat atagtcaata ttgcaattaa ttgcatttct aatgaagaag aaaactcact
     1621 ctattatgtt tatccctata ttcatgggaa acgcctcttt gctgaaccag agcaaataat
     1681 aacccttgca actgcgttag caaaattaca tcttaaaaaa aaatcttatc ctgaccaaca
     1741 gttgattatt aaaaatacca cggaacgaac atcacagctt aattttataa gaaaggcttt
     1801 agctgatgga tgttattctt acattccaca tttctctttt gtcaaaaaga tggctcaaca
     1861 atatgatttt gattggatta atcaagaaga tgcccaacca attcatggtg atttaaatgc
     1921 aggaaatctg ttactatccg aagataatat gatttgcttt tttgactttg aagacgcatt
     1981 acatagtttc catccagtag tgttagattt gctattcgtt attgagcgca ttatttttaa
     2041 tcaaaatagt tcgactgaac agctattaaa ccttggtctc atgtttattc atgcatataa
     2101 agaggctggt ggtacatacc agtataaaac gagagatgaa tttggattaa caattcttgc
     2161 tttaaaagca ttttgtttgt tgactttgct agcagagaaa aataaaaata tacttaattc
     2221 agaatgggat aaatttttta aattaactca aaaagcagga aatgagcggg atttaataaa
     2281 aacaatttta caaggataag aaagatagtg tgaaaattct tgttactgca cgggatgttg
     2341 gtgctgcatt aaacattatt gagatagtaa aaatacttaa gcaatatgca ggtgttactg
     2401 tatttattta cgctcaaccc cctgcttcaa aatattttct tcgggctggg attcaacctg
     2461 tatttgaagt tccactacca ccaagccgat ccttaaatga agccagtgac ctcctatcct
     2521 acgccaatca attaattgat aaattaaatc ccgatatcat attatctgga ttatcatccc
     2581 ctggcgaagc aggcatagat gaagcaataa tagctgagtg tcctgcgcat atcaaaacat
     2641 tcgtccttca agatttttgg ggagaagtta attttttctt tgaaaaatta gctgattgct
     2701 atttatgcat agatcatcac gctgcagaaa ttacaaaaaa acgatttaat gccaaaaccg
     2761 aggtgatagg ctcccctcgc catgccattt atcaaacatt aaatattgag gaacaacgca
     2821 actatataag agaatatttt aacattactc aaagtacaaa aactattggt ctttttgggc
     2881 aagcacttca tggtatacct ggatatatgg aaaccatttc acaatggtta gaagcatgct
     2941 tacaacaaga aaaaaccaaa ttagttattc tttatcgccc tcatcccaga gaatcagaag
     3001 ctgacaaaga agaaaccata agcttactta tttcatctgg attagaatgg aaaattttgt
     3061 cgactcaatt agttgaagaa tcattgttag cttgtgactt ggtttgtagc tttttttcta
     3121 attgccttta cgatgcagcc tatttaaact acttttcttt acgacctata ctaaccccgg
     3181 tcgtgcttaa ttttcaccca cagattaaaa attacactaa tcaattgagt ttatatgaaa
     3241 tttccccata tcgcaaacaa gagttagcct ttttttgtga ataccctcac acattagcaa
     3301 aggatattga ttacttatta tcaagcgaat gttcacataa atattggctg cacagtaaag
     3361 ttaaattaga acacccgact aaatccttaa ctagagaatt agctatctta ttaacaggag
     3421 aaccttaaat atgccatcaa ttatatctga caatcctaaa aattggtctg aagaaattaa
     3481 aaattacttg tgccgttctt ctcctgagcg ttatgaaaaa atggctacaa ataatgctat
     3541 tgaaccatat atatcaaaag ctatttcttg gggatatgcg gaatggaaaa ataggaaaac
     3601 attgcttgat gatattgaaa ctgaaatgtt gaggctagct acacttccag atttaagcga
     3661 ttttaatgat aatgaaatat actccagcaa aaaaaactac ctatcaaaag aaatactaga
     3721 gaattatttg ataaaagcct ccccagaaaa tttcaatact gtgtttcgcc aacccggttg
     3781 gaaatcacat gtcaattttt tgatcgagaa aggaaataga gaattagagc gacaaataat
     3841 caatggcaat ggtgtagaaa attcggttga tacagtctgt gcagagctcg caaaattaat
     3901 gaatgatttt gaaaactctt tttttgatga aatttatgga aactcaaccc cacgatatct
     3961 actacctaat ctatttgaaa aagcaatagc tgaatataaa ttattagcca ccataaagga
     4021 gcgaacacga tttattgagg catttattga aattgtgatg ttcgattggc aatttagcgg
     4081 aactttggat tctgatctat ttaagaatgc ttatagtaag gaagttctct attattatgg
     4141 aattggagca cgactttttg tacttggtga atttaaagaa gcgcaagaat attttaaaaa
     4201 aataattaat atcgagccca aacccattga ttatctaatg ctttcaaaaa catacagagc
     4261 attaaatgaa aaagttgaaa cttttaatac gatcaaggat ggattaacac attttcctaa
     4321 tgatttttta ttaacactag ccctttccgg taccctgtat tggatgggag aaacaaaact
     4381 agcaaatgaa acattagagc cagtcaaaaa agacttcaat actatgtata aatctgaaat
     4441 agaaggtgca aaaaaactag cagtagaaat ttcggaagca attaaatgcg ataaaattta
     4501 tcgagaccaa aagaatgata tttatgatga taaatacaca aaaaaagcat ggtaccaata
     4561 ttggtatcat tacaattgct ttcgacctac tcaacaccct gaagcctaca tgattgacta
     4621 tattccaaga tatcttacat ctatagccaa agatgccaca ataacattga aaggagtatt
     4681 agattttgga acactttgcg ctgaaccact ttatcgtttt gctaaaaata atccagagat
     4741 ccaatgcgtt ggaatagata ggcaggattt ggttaaagaa ctaaacgata aggcctattc
     4801 attaaataac ctccgttttg aagctggtga tatttttgaa tatcttgaac attttgcact
     4861 tttagtacca aatcatcgaa tgttatttca ttgtagaaca gctgtattgt gttaccctaa
     4921 ttttttgtta aagctatata aaaaatgtgc tgaacttggc attgaatata tatgccttat
     4981 ggagtctaac tctctcagta gagatacttt aacattttat aattatggag aaatgccaaa
     5041 tatttctata gcacgaagag ggtctctaat taatcatgat tattttcttt tgctagaaca
     5101 atccggatac gaaattgtca agaaagattt tcaccttgca cctcacctac tggaggatta
     5161 tgcagcatta ataggaagta ataccacttt ggttgctcaa ttgaaattat tgacacagag
     5221 ccaataatcc ccaaagaata aatgctgtta taacagcatt tattctattt ttgacaaata
     5281 taatcattct tatgagtgtt agactactaa tgcctcttaa attaaggcta actcattatt
     5341 tcagaataaa gagttaattt agtacttgaa agaatttttt tagccataat ttcggcctgt
     5401 gcaactacat catctggata acccctatga ccttcgacta tctttgcaag ctcatgcttg
     5461 taatgtgcag cgactataaa ccaagcaaaa gtttcaacta acgtagtagc ccctccttca
     5521 ccccaagcta ttactactga ataagctgat aaagctcccg gattagaggc gtatcgtgat
     5581 tctgcataag ggatatcatc tgggcaagtc ccgtctcctt gcatagatcc atttcctgga
     5641 atccatttta ttgcgacatt ccctcccaaa tcaacatttt caaattttgg atgttttcgt
     5701 tttacaaaaa gggcacatga tacatttgta gcaatattac ctctgaataa tccaaatgga
     5761 ccaagcgcat aatttgcacc tgaaatgata ttattttcta ttagagggta ttctcctttt
     5821 ctaatttagt ccaaattcca aaatttcttc cccaagttaa ccatttatca aatacaatat
     5881 cattattact tattctacaa cctgaaccca tatacaatgc tatcccgaca ttttcaccgt
     5941 tccttgtagc attacccaat tttattagat tccgctcaat tgagcataag ttacctactg
     6001 tgtaaattcc tgtggcaaaa gcatgagagt aggttc
//
LOCUS       DAXLDT010000174         5981 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXLDT010000174 DAXLDT010000000
VERSION     DAXLDT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.174"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..300
                     /locus_tag="ACF2SP_002666"
     CDS             <1..300
                     /locus_tag="ACF2SP_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFD2360436.1"
                     /translation="EFLLYTNDIVTGYDEFLSSHQQYYATEIQYQVEYDEETLLEQGE
                     KIAGRVFITTTRPNEPAKRIEVILIAQYKDEKIYRLWELTYPDWSKLPAFSNQLT"
     gene            525..1079
                     /locus_tag="ACF2SP_002667"
     CDS             525..1079
                     /locus_tag="ACF2SP_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HFD2360437.1"
                     /translation="MKGIIFNEFLNFVEKSESYTLVDQIIMDSHLKSNGAYTSIGTYS
                     PEELFQLVKALAMKNGKPTSVILQEYGEYLFEVFAKKYPQFFKEKKSVFQFLEALETH
                     IHFEVKKLYDHTELPHFECQYHSQKQMEMIYTSSRPLADFAEGLIRGCIKYHKENMTI
                     VRENLPAKTGFKVRFVLTKGDPDE"
     gene            1072..2349
                     /locus_tag="ACF2SP_002668"
     CDS             1072..2349
                     /locus_tag="ACF2SP_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2360438.1"
                     /translation="MSDYEVLLRHLDREIRARKEAEEISEKKISELYRFAQRLEESLR
                     AQKEISKKLKEKTKALKQQIAKTEEERHAKSTLQTILESAIEYSIIAINLKGVILVWN
                     EGARRNYGYEAQELVNKESIEILHTVEDIQSGKVRKFFQTVRREGKAEEIFQRVRKDG
                     SQFTASVTMTIHHDSQGKKVGYVIISKDITEIKEFEEKLIKSNQDLEEFSYIISHDLK
                     APLRAIIQLATWIEEDCGNKLDLLSRKNLQLLRERTRRMSGMIDGLLQYARAGRVNME
                     VEWVDTKELIQEIIDILNHDEQFTIIIHGSMPQLPTAKLLLSQVFSNLIANSMKHHHC
                     KTGTIEIGVVDKGTFYEFFVADDGPGIEPVYFEKIFRIFQTLKPKDEAETTGIGLTIV
                     KKIVESQNGKIEVDSELGKGARFSFTWPKVPHQ"
     gene            2413..2820
                     /locus_tag="ACF2SP_002669"
     CDS             2413..2820
                     /locus_tag="ACF2SP_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2360439.1"
                     /translation="MDLAADKVDILYLEDDEVDIQSVERVFHKISSLIKIEIAKSGNQ
                     ALDMLYGRNKENKIHPKLILLDINIPKMNGIEFLKELRDDSSFTDIEVFVLTAAYTSK
                     DKLAFEPLNIRGHLIKPLDYGEAIKLFWILQSM"
     gene            complement(2832..4253)
                     /gene="ankD"
                     /locus_tag="ACF2SP_002670"
     CDS             complement(2832..4253)
                     /gene="ankD"
                     /locus_tag="ACF2SP_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkD/LegA15"
                     /protein_id="HFD2360440.1"
                     /translation="MLTPPPDSKITTTDKSLDKLSIPMDMLKQMNESTMEQTKLDELR
                     KKMSLQTEILNKAKADNDMFFRLLIELMSLKLQGELFKEQLSKISKESGYDSVQSALI
                     QATNSEGQSPLQYALQKQDFTTAKYFLDNGAKAGPIEKAVFEIALDSKAAKEFGFPPL
                     PPEKEKLHPVKNFGLVLGIKTTSVDGTPSQFGHIAPTYQLMTDSVSHFAKSHPGNKNF
                     QEIANAFHFSNEASAFKFSTPQRNPEAGQDLSRRIQGGELTTIPVSCKGHAMGLSYVP
                     DGPGSKSGYLVYTNRGLGAKSHEHGTHIFRIDDSSKITSEFINNMTNGHSNGASHDEI
                     MSQIKAAAGNKEPIHHIKQKGQKNDNCTIANSKSNIEGILLCQKAREVGGFDKLTESD
                     MDSVKKEYKEYTKHMRVEKVNELAKALKENPRDPDLNNLTKEYLKQHPKADPKLKETL
                     ETALKQASESSMELSQPGTYKTI"
     gene            complement(4452..5081)
                     /locus_tag="ACF2SP_002671"
     CDS             complement(4452..5081)
                     /locus_tag="ACF2SP_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360441.1"
                     /translation="MTKIVYLHNDPDKMLYYKDSVPKDLKVVEPDAKMMLEILEQKLK
                     LLEVVDLDWFYCAICMELPKEPVFYPTSEGITNLYEKSELVKWFSRDLKNKSDPLGLG
                     SNVTISQYKTPTPKELLDFVIKSTGFAEDVFIDHYKKPLTKKVSPEEELLLEKPDNTI
                     RNEIDRPSHSKQRSCLSLLLYSIFGCKDNRKVTQEKPESLTSDHLPPHH"
     gene            complement(5242..5688)
                     /locus_tag="ACF2SP_002672"
     CDS             complement(5242..5688)
                     /locus_tag="ACF2SP_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360442.1"
                     /translation="MLHFKWCEFSELTSKQLYDILALRTSVFVVEQHCPYLDPDGRDF
                     FSMHLLGLEENSLVAYLRLFLPTEIENHLTFGRVVTAKSARTKGYGRKLIRELLSYCD
                     MHYPGIRIQCSAQNYLKKFYEEFGFKSYGSVYEEDGIPHIAMQRTL"
BASE COUNT         1886 a         1003 c         1200 g         1892 t
ORIGIN      
        1 gagtttctgc tctataccaa tgatatagtg acaggctatg atgaatttct aagctcgcat
       61 caacagtatt atgcgactga aattcaatat caagttgaat acgatgaaga gaccctcctt
      121 gagcaaggtg aaaaaatagc tggccgggta tttattacca ccactcgacc caatgagcca
      181 gccaagagaa tcgaagtcat tttgattgcc caatataaag atgaaaagat ttatcgatta
      241 tgggagttaa cttatccgga ttggtccaag ttgcctgcct tttcaaatca gctaacctag
      301 aggattctct tctcctccaa acaggggaat atcagaaaag tagttggtca agatttcccc
      361 gctttacatg gtgtagcgcc acgcaggagc gagcaagact tcatggttta atcaatatga
      421 gcaaaaaatg aaaaacattc ctgcttgcgt caggattata ttattgataa caaatttacg
      481 actatactta tagtattaat caattgatat aaaacatctt gtctatgaaa ggaatcatat
      541 tcaacgaatt tctcaatttt gtagaaaaaa gtgaatccta caccctggtg gatcaaatta
      601 ttatggatag tcatttgaag tccaatggtg cctacacgtc tatcggtaca tactctccgg
      661 aagagttatt tcaattggtt aaagcgcttg ctatgaaaaa tggcaaacca acatcagtga
      721 ttttacaaga atatggtgag tacttatttg aggtttttgc aaaaaaatac cctcaatttt
      781 tcaaggaaaa aaagtcggtg tttcaatttt tggaagcgct tgaaacacat attcatttcg
      841 aagtgaaaaa attgtatgac catactgaac taccccattt tgaatgccaa tatcacagtc
      901 aaaagcaaat ggaaatgatt tacacttctt cacgtccttt ggccgatttt gcggaaggtt
      961 taataagagg ttgtattaaa tatcataaag aaaacatgac tattgttcgt gaaaatctgc
     1021 ctgcaaaaac aggttttaag gtaagatttg tattaacgaa aggcgatcct gatgagtgat
     1081 tatgaagtat tgttacgcca tcttgataga gagatacgtg caaggaaaga agctgaagaa
     1141 ataagtgaaa aaaaaatatc tgagttatac cgttttgccc aacgtctgga agagagttta
     1201 agggctcaaa aagaaataag taaaaaatta aaagagaaga ccaaggcatt aaagcagcaa
     1261 atcgctaaaa cagaagaaga acgacatgcc aaaagtacat tacaaaccat attggaaagt
     1321 gcaattgaat attcaatcat tgcaatcaat ttgaaaggcg tcatcttggt ttggaatgaa
     1381 ggggcaagac gaaattacgg ctacgaggcc caagaattag tgaacaaaga gagcatcgag
     1441 attttgcata cagtggaaga tatccaatca ggtaaagtaa gaaaattttt tcaaacggta
     1501 cgtcgggaag gaaaggcaga agaaatattt caaagggtca gaaaagacgg gtcccagttc
     1561 actgcttccg ttactatgac tatccaccat gattcgcaag gaaaaaaagt agggtacgtc
     1621 attatttcaa aagatattac agaaattaaa gaatttgaag aaaagctgat taaaagtaac
     1681 caggatcttg aggagttttc atacattatt tcccatgacc taaaagcgcc actaagggca
     1741 attattcaat tggcaacatg gattgaagag gattgtggaa ataagttgga tcttctttcc
     1801 agaaaaaatc ttcaattgct tagagaacgt acccgtcgca tgagcgggat gatagatggc
     1861 ttattacaat atgcgcgggc aggcagggtc aatatggagg tagaatgggt agatactaag
     1921 gagttaattc aagaaataat agatattctt aaccatgatg aacaatttac tatcattatc
     1981 catggatcca tgccccaact gccaacagcc aaattacttc taagccaggt tttttctaat
     2041 ctgatagcaa atagcatgaa acatcatcat tgtaaaacag gaacaattga aattggtgtt
     2101 gtggacaaag gcacatttta tgagtttttt gtggcagatg atggtccggg tattgagcca
     2161 gtatattttg agaaaatatt cagaatattt caaaccttaa aacccaaaga tgaggcagaa
     2221 actaccggga tagggttaac tatagtaaag aaaattgtag aatctcaaaa tgggaaaatt
     2281 gaggtggact ctgagttggg taaaggcgca agattttcat ttacatggcc taaagttcct
     2341 catcaataaa ggtttaggtc tataattaat ttagaaataa aaaaataaaa cattgtctca
     2401 cggagaatag tgatggacct cgcagctgat aaagtagata tcctgtattt ggaagatgat
     2461 gaggttgaca ttcagtctgt tgagcgcgta ttccataaaa tcagttcatt aatcaagatt
     2521 gaaatagcta aaagtgggaa tcaggcattg gatatgctat atggtcgaaa taaagaaaat
     2581 aaaattcatc ccaagttaat tcttctcgac atcaatatac ctaaaatgaa cggtattgag
     2641 ttcttaaaag aactaagaga cgactcttcc tttacggata tagaggtatt tgtacttacg
     2701 gcagcctata ccagcaagga taagctggct tttgagcctt taaatattcg aggccatttg
     2761 attaaaccgc ttgattatgg cgaggctatt aagctatttt ggatattaca atccatgtag
     2821 acagtgtggt attaaatggt tttgtaagta cctggttgac ttaattccat actgctttca
     2881 gaagcctgtt ttaatgctgt ttctaaggtt tcttttaatt tagggtcggc ttttggatgt
     2941 tgttttaaat attctttggt caaattattt aaatcaggat cacgaggatt ttctttaaga
     3001 gctttggcta attcattgac tttttcaact ctcatatgct tggtatattc cttgtactct
     3061 tttttaacgg agtccatatc gctctctgtt aatttatcga accctcccac ttctctggct
     3121 ttttgacata atagtattcc ctctatgttg gatttggagt tggctattgt gcaattgtca
     3181 tttttttgtc ctttttgttt gatatggtga atgggctctt tgtttccggc agctgcttta
     3241 atttgagaca taatttcatc atgagaggca ccattcgaat gtccattagt catgttattg
     3301 ataaattccg aggtgatttt actggaatca tcgattcgga aaatatgggt gccatgttca
     3361 tggctctttg ctcccaagcc gcgattggta tataccaggt agccagattt ggagccaggg
     3421 ccatcgggta cataagacaa acccatagca tgtcctttac agcttacagg aattgtggtg
     3481 agttcgccgc cctggatacg tcttgaaaga tcctgaccag cttccgggtt tctttgcggt
     3541 gtgctgaatt tgaaggcaga tgcctcgttt gaaaaatgga aggcattagc tatttcctga
     3601 aagttcttgt tgccaggatg agatttggcg aaatgactga cagaatctgt catcaattga
     3661 taggtaggcg ctatatggcc gaattgagag ggagtgccat ccacactggt tgtttttata
     3721 cccaaaacca gaccaaaatt tttgacagga tgcagttttt ctttttcagg tgggagtgga
     3781 ggaaagccaa actctttagc tgctttactg tcaagggcga tttcgaagac ggctttttct
     3841 atcgggcctg cttttgctcc gttatccagg aaatattttg ccgtagtgaa gtcttgtttt
     3901 tgcagcgcgt attgtaaagg gctttgccct tctgagtttg tagcttgaat taatgcgctt
     3961 tgtacgctgt cataaccact ttctttagaa attttgctta attgttcttt aaataattca
     4021 ccttggagtt ttaatgacat taattcgata agtaaacgaa aaaacatatc attgtctgct
     4081 ttcgctttat tgagtatttc ggtttgtaga gacatttttt ttcgtagttc atcgagtttg
     4141 gtttgctcca ttgttgactc attcatttgt ttaagcatat ccattggaat acttaattta
     4201 tccaaacttt tatcggtggt tgtaattttt gagtcaggtg gaggagttaa catggaacac
     4261 cctgattgat tagttaaact atataattat tatacatcag gacttacgaa aaaccccaat
     4321 cttcttgtta aaaaatgttt ttaattcagt cattcagttt atcgaaaagc tctcattaaa
     4381 cccattgttt gtcttggtct tgggggggcg taagtcctgt atataaaaac cgtctaaaaa
     4441 ctttattttt attaatgatg aggtggcagg tggtccgatg ttagcgattc gggtttttcc
     4501 tgcgttactt ttctgttgtc tttacaacca aaaatactat acaacaagag actcaaacag
     4561 gatctttgct ttgaatgaga gggcctatct atttcatttc gtatggtatt atcgggtttc
     4621 tcaagtaaga gctcttcctc cggggaaact ttttttgtca gtggtttttt gtaatggtca
     4681 ataaacacat cttcagcaaa tcctgttgat ttaataacaa aatcaagcaa ctcttttgga
     4741 gtaggcgttt tatattgaga tatagtaaca tttgaaccaa gtccaagggg atcggattta
     4801 ttcttgagat ctcttgaaaa ccatttgact aactcacttt tttcgtaaag attggtaatg
     4861 ccttctgagg ttggataaaa tactggctct ttaggaagtt ccatacaaat tgcacaataa
     4921 aaccaatcca aatcaacgac ttcgagcagt tttaattttt gctctaaaat ttctaacatc
     4981 attttggcat ctggctcaac cacttttaaa tctttgggga cgctgtcctt ataatagagc
     5041 attttatccg ggtcattgtg tagataaaca attttcgtca taacctctcc aaagaataat
     5101 actttaaata acgtgagtta tggataaaga tgattaatga gtaaacatga caaaaagtgt
     5161 gctttattgt ggcactgatg tcaatttaaa atttgatcgg gctggtagat ttacaggaaa
     5221 cccggattgg ttatggcggt tttataaggt tctttgcatg gcaatatggg gaataccatc
     5281 ctcctcataa accgagccat aacttttaaa gccgaattct tcatagaatt ttttcaagta
     5341 attttgggca gagcattgaa tgcgaatacc agggtaatgc atgtcgcaat aagacaataa
     5401 ttctcgtatc agtttccttc catatccttt ggttcgcgct gatttagctg taacaacacg
     5461 gccaaatgtg agatggtttt cgatctcagt tggaagaaac aggcgcaaat aagccacgag
     5521 agaattttct tccaggccca ataaatgcat ggaaaaaaaa tctctaccgt ctggatcaag
     5581 gtaggggcag tgctgttcta ctacaaaaac gctcgttcgt aatgccagaa tgtcatagag
     5641 ttgtttgctt gttaactctg aaaactcaca ccatttaaaa tgaagcatga aacactccca
     5701 atccggctaa aatcttcgat gcattatgct gaagttatga ctctgaaatc aagtggagta
     5761 atgatcagga tgaaaaaaac tcaatttctt tgttaaaaaa atgtttttaa ttaagttatt
     5821 tagtttgaat aatcaccata attaaaaaca ttttttgcct ccagctggat gtttttcgca
     5881 agtcccgatt atatcagggc aaatttaagg gcaatggatt gaagtaaaaa tcattatcga
     5941 aattatcttt agtaattaat gcaaattcat atattatggt g
//
LOCUS       DAXLDT010000175         5924 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXLDT010000175 DAXLDT010000000
VERSION     DAXLDT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.175"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(232..726)
                     /locus_tag="ACF2SP_002673"
     CDS             complement(232..726)
                     /locus_tag="ACF2SP_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFD2360443.1"
                     /translation="MSHFSILVEEIAAYLKKAKWQLVTAESCTGGLIAACLTEIPGSS
                     NWFERGFVTYSNLAKEEMLSVAHELIQTHGAVSEEVALAMAAGALQHSMGNIAVSVTG
                     IAGPEGGSIEKPVGTVCFAWAVHHQFQQTSRQQFTGSRQEIRLSACQYALNGVLSTLK
                     KVCG"
     gene            complement(803..1000)
                     /locus_tag="ACF2SP_002674"
     CDS             complement(803..1000)
                     /locus_tag="ACF2SP_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360444.1"
                     /translation="MNRLAMIAAASCLALFLTGCGEGSKPADTKTDQQQQQQQPTDQQ
                     QQGGENTGNNQAQPSTEGQGQ"
     gene            1347..3887
                     /gene="mutS"
                     /locus_tag="ACF2SP_002675"
     CDS             1347..3887
                     /gene="mutS"
                     /locus_tag="ACF2SP_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFD2360445.1"
                     /translation="MSSSHTPMMQQYLRIKTDYPDMLLFYRMGDFYELFFDDAKRASQ
                     LLDLTLTHRGQSADKPIPMAGVPYHAVENYLARLLKKGESVAICEQIGDPATSKGPVE
                     RQVTRIITPGTVTDEALLDARKDNILLAIHTQKQKIGIAWVDLGGGRFHLQELTEEHQ
                     LNAELVRLQPAELLCKESTPLPSFCSNFAVKFRPGWEFDASNAHKLLCEQFSVTDLSA
                     FGEQNYPTALIAAGALLAYLKTTQKQSLPHLTTLTLEQSEDYLQLDASTQKHLELFEN
                     IHGGGEHCLLSILDKTACAMGSRLLKRWLGKPLKQHAIIQTRQQAIKEIIFLQQDVSL
                     HQLIKQCADVERIVSRIALKSARPRDLVSLLQTLTLLPAIHDELQENKSLLINEIKKE
                     ISPLPLLQQLLETAIIDNPPMLIRDGGVIAPGFDEELDELRNLSSNAHETLVKLEQEE
                     KNRTGLSTLKLGYNSVQGFYIELSKAQAQNAPPHFHRKQTLKNVERYITPELKLFEDK
                     VLSAQSKALAREKWLYDNLLEEIQQYIPELSDLAKSLAQLDVLVTLAERAQSLNWNCP
                     NLVPESGIMIQAGRHPVIEPLLQERFIANDLELKPNQNMLLITGPNMGGKSTYMRQTA
                     LIVLLSHIGSFVPADEVTLGPLDRIFTRIGASDDLSSGRSTFMVEMTETAQILRQATS
                     QSLVLIDEIGRGTSTYDGMALAYASCAFLASTIKAYTLFSTHYLELTELPKEFSCIRN
                     VHLQASIKTGQIVFLYRVEEGCANRSYGLEVAELAGIPKEVLKLAHEHLNQIQDTQSI
                     LVQTQIIKPPTSPVLTELKKIDPDRLTAKEALDLIYKLKHLECAESIN"
     gene            3900..5588
                     /locus_tag="ACF2SP_002676"
     CDS             3900..5588
                     /locus_tag="ACF2SP_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFD2360446.1"
                     /translation="MNRFAYLIILIILCFPLFAQDKKAGLFEIDGIKGKVLANVETRL
                     DELSQIKPLSQFTPTELQDQINKAIQPFGYFNAETSINNLNNKIIIKVQPGSQIRIAS
                     IKAMLTGEGAQNPLLRKTLKELPLHIGDPLFSEQYEKAKQNIINTAENMGYLHGVFKK
                     AEILIDEHKKSAQITLIFDTGPQYYFGQVQFDPTYISPQLLHRFVPFDPGQPYATDQV
                     LKLNDYLSNSGYFSSVLVKPQITDAQTVPVIVHLQPVPKYSYSFGLGYGTDTGVRGKA
                     ALHVIPVNRQGHKFNAVAQGSFRQNALQAQYVIPGKNPVTDQYALTGNFSNLNYNAGY
                     SNATLLSLSQLHNVNQFQRALSLNALYESFHYSLQPNTDQFLLYPKANITFSKTKNLL
                     FSPSGYNITFNALGANKAVLSHLNFGQLSLDAKAALTLDSLHLRLYGHTIQGITAIND
                     INELPLSLALLLGGTDNLKAYSFNSIGPGKIITYGGFEIQKEFKKNWYLVGFYDAGDV
                     YNPSVKNIQYDIGGGLMWVSPIGPIKVGLAQSVDNKMERIGHNPRLVISMGPDL"
     gene            5585..>5924
                     /locus_tag="ACF2SP_002677"
     CDS             5585..>5924
                     /locus_tag="ACF2SP_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF490 domain-containing protein"
                     /protein_id="HFD2360447.1"
                     /translation="MMILKFIKKGFYYCLITAICLVSLIIFLISTTPGLYAVIKLTHL
                     YLPGTLKIHHLKGQLLDQFSIGEMEYQHQGTKIKIFNLAVNWRFSSLLHKKLPIDSMS
                     AEMIEINQKTP"
BASE COUNT         1912 a         1209 c         1058 g         1745 t
ORIGIN      
        1 gtttaaaaca agaaacacat tttcgaggat ccgtagattt tcattgtata aatccattct
       61 acgaactatt tgcgttacag ctataaaaca aaagacactg tttatgcaag aggtttattg
      121 taatttttat tagctgagtg gtaaaaacaa gaatttgaga ccaaataata gaattgaaaa
      181 aatatagact attgatgttt tattaaacta tgtttttaaa aaaccattcg gttagccaca
      241 gactttttta agagtagaaa gaactccatt taaagcatat tgacaggcag acaaacgtat
      301 ttcttgccga cttccagtga attgttgtct tgatgtttgt tgaaattgat ggtgaactgc
      361 ccaggcaaag catacagtac ctactggttt ttcaatactg cctccttctg gacctgcgat
      421 accagtaact gatacagcga tattacccat actatgttgt aacgcaccag cagccatagc
      481 caaagccact tcttcactga ctgccccatg agtttgtatt agctcgtgag caacagaaag
      541 catttcctct ttagctaaat tactataagt aacaaaacca cgctcaaacc aatttgagct
      601 tccaggtatt tccgtaaggc aagcagcaat taaaccacca gtacaagatt ccgcagtcac
      661 taattgccat ttggcctttt ttaaataggc agctatttct tcaaccagta tcgagaaatg
      721 actcatacct ttcacctata aaaaacccca taaaacatct atggggtttt ttgataattt
      781 ataaataaca ctgaaattaa tcttactggc cttggccttc agtagaaggt tgggcttggt
      841 tatttccagt attttcacca ccttgttgtt gctgatcagt tggttgttgt tgctgctgtt
      901 gttgatcagt ctttgtatct gcaggcttcg agccctcacc gcatccagtt aaaaataacg
      961 ctagacaact agcagcagca atcatagcta aacgattcat aatcactctc cctatttatt
     1021 ttttgtaata tcacgttaat tctagcaaat attctcatta agtgaagaac ttatctcctg
     1081 aacttaaaaa ataaaattta tattttattg cagtatgtta aattaaatga cataaaccat
     1141 gccttagtaa tcattttggc aaattctata gtaatttgca gattttaaaa atcatactta
     1201 ttttttagac acatcaaggt tatacttaag gttttttgat cacattgtct ttattgtttt
     1261 cataaaaacc aataccacca acatgcctta ctgacttcac acaacacttt tgttaaaatt
     1321 gaggcatttc aatcggctca atcactatgt ctagctctca cacaccaatg atgcagcaat
     1381 atttgcgcat taaaacagac tatcccgaca tgctcttatt ttatcgcatg ggtgattttt
     1441 atgagttgtt ttttgatgat gccaaacgcg cgtcacaact tttggattta actctgacac
     1501 atagaggaca atctgctgac aaacctattc ccatggccgg cgttccttat catgcggtag
     1561 aaaactattt ggcacgatta ttaaaaaaag gggaatccgt agccatctgt gaacaaatcg
     1621 gagatccagc taccagcaaa ggaccagtag aaagacaagt aacgcgaatc attactcctg
     1681 gtacagtaac agatgaagca ttattagatg ccagaaaaga taatattttg ttggccatac
     1741 acacccaaaa acaaaaaatt ggtattgcat gggttgattt aggtggcggt cgttttcatt
     1801 tgcaggaatt aacagaagaa catcagttaa atgcagagtt agtgcgcctg caaccagcag
     1861 agctcctctg caaagaatcc acgccgcttc cttcattttg ttctaacttt gctgttaaat
     1921 tcagaccagg atgggagttt gatgcaagca acgcacataa attattgtgt gaacaattct
     1981 ccgtgaccga tctttcggcc tttggcgaac aaaattaccc cactgccttg attgcagcag
     2041 gcgctttatt agcttattta aaaaccactc aaaaacagtc attaccacat cttacaacac
     2101 ttactcttga gcaatccgag gattacctgc aactggacgc atcgactcaa aagcacttgg
     2161 agctttttga aaatatacat ggtggtggtg agcactgttt gctttccata ttagataaaa
     2221 cagcttgtgc gatgggaagc cgtttactca agcgttggct gggaaaacca ttaaaacaac
     2281 atgcaatcat tcaaacaaga caacaagcca ttaaggaaat tatttttctt caacaagacg
     2341 tttcacttca tcagctgatt aaacaatgcg ccgatgtgga gcgcatagta tcacgtattg
     2401 ctttaaaatc agcgcgccca cgtgatttgg tttccttatt gcaaactctg acactgcttc
     2461 cagcaatcca tgacgaatta caagaaaaca agagcttatt aatcaatgaa atcaagaaag
     2521 aaataagccc tctcccactg ctgcagcaac tgcttgaaac agccattata gacaacccgc
     2581 caatgctaat tagggatggt ggagtcattg ctccaggatt tgatgaagaa ctggatgagt
     2641 tgcgtaattt aagtagcaat gctcatgaaa cattagtaaa acttgaacag gaagaaaaaa
     2701 atcgcacagg attatccact ctaaaactag gttacaatag cgttcaggga ttttatattg
     2761 aactgtcaaa ggcacaagct caaaatgcgc cgccccattt tcacagaaag caaactttaa
     2821 aaaatgtgga acgttatatt actcccgaac taaagctgtt cgaagacaag gtgctttcag
     2881 ctcaatccaa agcattggct cgcgaaaaat ggctatatga caatttgctt gaagaaattc
     2941 agcaatacat acctgagtta tcggatcttg caaaatctct ggcgcaattg gacgtattgg
     3001 tcactcttgc tgaaagagca caaagtttaa attggaattg cccgaatctc gttcctgaat
     3061 caggcattat gatacaagcc ggacgacatc ctgtcattga gccattatta caagaacggt
     3121 ttatcgctaa tgatttggaa cttaaaccaa atcaaaatat gttactaatc actgggccca
     3181 acatgggcgg caaatcgacc tacatgaggc aaaccgcctt aatcgttctg ctgtcacata
     3241 ttggaagttt tgtacctgct gatgaagtga cattaggccc attagaccga atctttactc
     3301 gcattggggc cagtgatgat ttatcctctg gacgttccac ttttatggtg gaaatgactg
     3361 agacagctca aatattaagg caggcaacca gccaaagcct cgtattaatc gatgaaattg
     3421 gtcgtggtac cagtacctat gatggaatgg cattagctta cgcaagctgt gcctttcttg
     3481 cgtcaacaat aaaagcctat actttgtttt ctactcatta cctggagttg accgaattac
     3541 ccaaagaatt ctcttgtatt cgcaacgtgc atttgcaagc atccatcaag acaggacaaa
     3601 ttgttttctt gtaccgtgtc gaagaaggtt gcgcaaatcg tagttacgga ttagaagtcg
     3661 cagaattagc tggcatacct aaagaagtgc taaagctggc tcatgaacat ctgaatcaga
     3721 tacaagatac tcaatctatc ctggtccaga ctcaaattat aaagcctccc acatctccag
     3781 ttttaactga actaaaaaaa attgatccag atagattaac tgccaaagaa gcgttggatt
     3841 taatttataa gttaaaacac ctggagtgtg ccgagagcat caattgatgt agttattaca
     3901 tgaataggtt tgcatattta atcatactaa tcattttgtg ttttccattg tttgctcagg
     3961 acaaaaaggc cgggctgttt gagattgatg gtataaaagg aaaagttctt gctaatgtgg
     4021 aaactcgttt agatgaattg agccaaatta aacctttaag ccaatttacc ccaactgaac
     4081 ttcaagacca aattaataaa gccattcaac catttggata tttcaatgcg gaaaccagta
     4141 ttaataatct caacaataaa ataataataa aagttcagcc tgggtctcaa attcgtattg
     4201 cctccattaa agcgatgcta acaggagagg gagcacaaaa tccattatta cgaaaaactc
     4261 tgaaagagtt acctctgcat attggagatc ctctgttttc agaacaatac gaaaaagcaa
     4321 aacaaaatat tataaatacc gcagaaaata tggggtattt acacggcgta tttaaaaaag
     4381 ctgaaatatt gatagatgaa cacaaaaaat cggctcaaat caccctgatt tttgatacgg
     4441 ggccccaata ctattttggc caggtgcaat ttgacccaac ctatatttct cctcagctat
     4501 tacatcgttt cgtaccattt gatcctggcc aaccttatgc cacagatcaa gttttgaaat
     4561 tgaatgatta tttatcgaac agcggttact ttagttctgt actggtaaaa ccccaaatta
     4621 ctgacgccca aacagtaccg gtcattgttc atttgcaacc tgtacctaaa tattcctatt
     4681 catttggcct tggctatgga acagacacag gagtacgtgg aaaagcagct cttcatgtga
     4741 ttccagtcaa tcgccaagga cataagttta atgccgtagc gcaaggctct ttcaggcaaa
     4801 acgccttaca agcccaatat gtaatccccg gaaaaaaccc tgttaccgat caatatgcct
     4861 tgaccggtaa tttttcaaat ttaaactata atgcaggtta tagtaatgct actctcttat
     4921 ccctgagcca attgcataat gtgaatcaat ttcaaagagc gctttcactg aatgctctgt
     4981 atgaaagttt tcattactct ctacaaccca atacggatca gtttttgctc taccccaaag
     5041 ccaatataac tttcagtaaa actaaaaatc tcttgttttc gccatcaggg tataatatta
     5101 cttttaacgc gcttggggcg aataaggctg tcctctccca tctgaacttt ggacaacttt
     5161 cactggatgc caaagccgcg ctcacattgg attcactgca tttgcgcctg tatgggcata
     5221 ccatccaagg tattacagca atcaatgata tcaatgaatt accgctttcc ctggcattac
     5281 ttctgggagg aactgacaac cttaaagcct atagttttaa ctctatcgga ccaggaaaaa
     5341 ttatcactta tggtggcttt gaaattcaaa aagaattcaa aaaaaactgg tatcttgttg
     5401 gattttacga tgctggagat gtttacaatc catccgttaa aaacattcaa tacgatattg
     5461 gaggcggtct gatgtgggtt tcccctattg ggccaattaa agttggatta gcccaatcag
     5521 tcgataataa gatggagaga ataggacaca atccaagatt ggttatcagt atgggaccag
     5581 acttatgatg atattaaaat ttatcaaaaa aggattttac tactgcttaa taacagcaat
     5641 atgtctggtt agtttgatta tctttttgat ttcaactacc ccggggctat atgcagtaat
     5701 taaattaact catttatatc ttcctggcac tcttaaaata caccatctta aaggacaatt
     5761 acttgatcaa ttttctattg gtgaaatgga atatcaacat caaggcacta aaataaaaat
     5821 ttttaacctg gcggttaact ggcgtttcag ctcattgctt cacaaaaaat tgcctattga
     5881 tagcatgagt gctgaaatga ttgaaataaa tcaaaagacg ccca
//
LOCUS       DAXLDT010000176         5876 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXLDT010000176 DAXLDT010000000
VERSION     DAXLDT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.176"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(213..1130)
                     /gene="secF"
                     /locus_tag="ACF2SP_002678"
     CDS             complement(213..1130)
                     /gene="secF"
                     /locus_tag="ACF2SP_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFD2360448.1"
                     /translation="MEFFNPNSKIDFMGARRWTALFSILIFVVSIGALMINGLKWGLD
                     FTGGTQIEISYSKAADLPLIRENLNKAGFKEAQVVSYGTSKDVLISIAPRADKQQSML
                     VDQVMEVLPGAVKQRVDFVGPQVGQELATKGALAIIVSLLATMIYIAMRFEYKLAVSS
                     AVALIHDPVLILGVFALFGIEFDLKALAGLLAVIGYSLNDTIVVFDRVRENFVKIRRT
                     SPIDIMNISINQTLSRTIMTSVLTLFVVVALFVYGGEAIYGFSLALIIGIVIGTYSSI
                     YVAGTLAVAMGLDNKDFLPSHRREVDDRP"
     gene            complement(1143..2999)
                     /gene="secD"
                     /locus_tag="ACF2SP_002679"
     CDS             complement(1143..2999)
                     /gene="secD"
                     /locus_tag="ACF2SP_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFD2360449.1"
                     /translation="MQNKYPLWKNLMLIVIAVIGFIYAIPNLYTENPVVQISAGTDED
                     TETIKNQVESILKNANMDYKSLVVERDGVEVVFSSTDSQLLARDVIKNSLGSDYTVAL
                     NLAPSTPAWLEAIHADPMKQGLDLRGGVHFLLEVDVESVISRRYEGIMKNIGQELREA
                     GIRYSGIRYLSDKGIELRFRDSDSMENALVELRQKISDVVFVKTKTQNSILGAISPAE
                     LNSIRQNTIEQTMSILRNRVNELGVGEAVVQQQGATRVAVDLPGIQDAARAKQILGGT
                     ATLEFHLVDQENDPFIAKQTGVVPVNDKLYMMDGHPILLKRQVVLSGDSITSAVSSFD
                     QQTASPSVQIQLGGGGESLFTKVTRENIGKRMAIVFVETKTTTQTINGEEKRVTKKEE
                     RVISAPVIQNALGSNFQITGLTDSKEASNLALLLRAGALPAAIYPVEERTVGPTLGKE
                     NIRRGLISLEVGMGLILVLMLVYYHFFGLIADIALMLNLVFLCALLSIIDATLTLPGI
                     AGIVLTVGMAVDANVLIYERIREELRNGLSPQAAIHAGYERAFSTILDANVTTLIVAI
                     ILFAVGTGPVRGFAVVLSLGLLTSMLTGITYTRAIVNWYYGGRNVKKLSIGI"
     gene            complement(3021..3356)
                     /gene="yajC"
                     /locus_tag="ACF2SP_002680"
     CDS             complement(3021..3356)
                     /gene="yajC"
                     /locus_tag="ACF2SP_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFD2360450.1"
                     /translation="MSFFISDALAAAGNAPAAQTDGTFSLIMIVAIFVLFYFMIIRPQ
                     SKRAKEHRELINRLKKGDEIVTSGGILAKVVSLDDQYIKVSPAEGIEINLQKGAVTAV
                     LPKGTIKSL"
     gene            complement(3502..4335)
                     /locus_tag="ACF2SP_002681"
     CDS             complement(3502..4335)
                     /locus_tag="ACF2SP_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuine tRNA-ribosyltransferase"
                     /protein_id="HFD2360451.1"
                     /translation="MLSTNQNFVPVLNTEAGLCLTAANWQEIKITIASCYLDLLLLKP
                     GYSLLKNITDFTKYLGWSGHLILNASRLVSDKNGMVVLISPYDGSRIKLTNSELVHLI
                     LHIKPVAVLLPETLVNGFSGLWEQWDDTIFPFLSAKQLETLQVPGKHGVYFNFSEAKY
                     HDDFLSQLQKWSELPLYVSGPIGADLIEDLNRKKSILIESDEPAKNAMRGIVYGREGN
                     VDLTDESQAFNFQLIDENCSCPTCSAQLTRAYLHHLLANTPLLCQRFLIQHNAYYVQG
                     N"
     gene            complement(4382..5395)
                     /gene="queA"
                     /locus_tag="ACF2SP_002682"
     CDS             complement(4382..5395)
                     /gene="queA"
                     /locus_tag="ACF2SP_002682"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFD2360452.1"
                     /translation="MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFR
                     EIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLK
                     SNDLIYLDAGKRLEVLERQDDLFLCKASENILDLLNDLGHIPLPPYIAREDESLDKER
                     YQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQD
                     HKMHSEWFTVSPDLCAAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIF
                     IYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEAIDKRYRFFSYGDAS
                     LLL"
     gene            complement(5510..>5876)
                     /locus_tag="ACF2SP_002683"
     CDS             complement(5510..>5876)
                     /locus_tag="ACF2SP_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360453.1"
                     /translation="FFSQEFIRTFKKILAIKGAKLLVPLGIASWLIYNFDYLFIWVIY
                     YIREVLQAVLAFLTRIMPFKPYSTSIALIILLTTVSVGPVLLLDLIYRKRTYKGYAYP
                     YLTSTLILIFCTLILLVVS"
BASE COUNT         1883 a         1323 c          977 g         1693 t
ORIGIN      
        1 gcgcagcaaa aatagataag ttactggttg attaaaaatt attggctctt tttaagaaga
       61 cacattttaa caaaattttg taattgttta ttatccgctc cctggcagtc gcggctcggt
      121 ttcgtccata atccgatgtg gttcggagta aactatgatc cgagccgcga ccatcaggga
      181 gcggataatg catggacaat aagctctcca cctcaaggcc tatcatccac ctccctgcga
      241 tgactgggta gaaaatcctt gttatccaaa cccatggcta ctgctaaagt gccagcaaca
      301 taaattgaag agtaagtacc tattactatt cctataatca acgccaatga aaacccatat
      361 atcgcctccc ccccatagac aaaaagcgca accacaacaa acagcgtcaa cacagatgtc
      421 atgatcgttc ttgagagagt ttgattgatt gaaatattca taatatctat tggacttgtt
      481 cggcgaattt ttacaaaatt ttctcggaca cgatcaaaga caacaatggt atcattcaag
      541 gaataaccaa taacggccaa caacccagca agagccttca gatcaaattc aattccgaac
      601 aaagcaaaaa cgcccagaat caataccgga tcatgaatca aagccactgc cgaacttaca
      661 gcgagtttat attcaaaccg catagcgata tagatcatcg tggctaacaa ggacacaata
      721 atcgcaagag cccctttcgt tgctaactct tgtccaactt gcggaccaac aaaatcaact
      781 cgttgtttta ctgctccagg cagtacttcc atcacctgat ctaccagcat gctctgctgt
      841 ttatccgctc tcggtgcaat gctgataagt acatcttttg atgtaccata actaaccact
      901 tgagcttctt taaaacccgc tttattcaga ttttctctaa ttaaaggtaa atcagccgct
      961 ttggagtaac tgatttcaat ttgcgttcca ccagtaaaat ccaatcccca tttcaaacca
     1021 ttaatcatca atgcgccaat agacactaca aaaattaata tagaaaacaa tgcagtccaa
     1081 cggcgcgcgc ccataaaatc tatttttgaa tttggattaa aaaattccat ctgagtctcg
     1141 ccttatatgc ctatagataa ttttttgaca ttacgcccac cataatacca attcacaata
     1201 gctctggtgt aagtaatacc agttaacata gaagttaaca aacctagaga aaggacaacc
     1261 gcaaacccgc gcaccggtcc tgtgcccacc gcaaataaaa tgatagctac aattaaagta
     1321 gtcacgttgg catccaatat agttgaaaat gctcgctcat atcctgcatg aatagcagct
     1381 tgaggagaca atccattgcg caactcctct ctaatccgct cataaatcag gacattggca
     1441 tcaacagcca tgcccacggt caaaacaatc cccgcaattc ccggtaaagt cagtgtcgca
     1501 tcaattatgg acaacaatgc gcaaagaaaa actaaattca gcatcaatgc aatatcagca
     1561 attaaaccaa aaaaatgata atacacgagc attagtacca aaattaaacc cataccaact
     1621 tccagggaaa taagtcctcg gcgaatattt tcctttccaa gagttgggcc aacggtacgc
     1681 tcttcaacag gatagattgc agcaggtaaa gcgccagctc ttaataataa agccagatta
     1741 cttgcttctt tgctatcagt cagtccggta atctggaaat tgctgcctaa agcattttga
     1801 atcactggcg cactaataac tcgctcttct ttctttgtta ctcgtttttc ctcgccatta
     1861 attgtttgtg tagtcgtttt ggtttccaca aaaacgatcg ccatgcgctt accgatattt
     1921 tcacgcgtaa ctttagtaaa caagctctcg cccccgccac ccaattgaat ttgaacagat
     1981 ggagaagccg tttgttgatc aaaactggac actgctgacg taatagagtc gccacttaaa
     2041 acgacctgtc tctttaataa gataggatga ccatccatca tatacagctt atcattgaca
     2101 ggaactacgc cggtttgctt tgcaatgaaa gggtcatttt cctgatcaac caaatgaaat
     2161 tccaaagtag cagtaccacc aagtatttgt tttgctctgg ccgcgtcttg aatgcctggc
     2221 aaatctacgg cgactcgagt cgccccttgt tgttgtacga cagcttcacc aactccgagc
     2281 tcgttgacac gatttctcaa gatactcatt gtctgctcaa tagtattttg tcttatggaa
     2341 tttaattctg ctggagaaat ggcgcctaaa attgaatttt gtgttttagt tttaacaaaa
     2401 accacatcag atattttttg tcttaactca actaaagcat tttccattga gtcagaatct
     2461 ctgaaacgca attcaatccc tttatctgat aaataacgta tgcctgaata acgaatacct
     2521 gcttcacgta attcttggcc aatatttttc attattcctt cataacgccg acttataaca
     2581 ctctcgacat ccacttcaag aagaaaatga acaccgcctc ttaagtccaa cccttgcttc
     2641 atagggtcag catgaattgc ttcaagccaa gctggtgtgg aaggcgctaa attcaaagcc
     2701 actgtgtaat cagatcccag gctattcttg ataacatcac gagctaataa ttgagaatca
     2761 gtagaagaaa atactacctc taccccatcc ctctccacaa ccagggattt ataatccata
     2821 ttggcatttt ttaatatgga ctctacctga ttttttattg tctctgtatc ttcatctgta
     2881 cccgcagaaa tttgaacaac gggattttcc gtatacaaat tcggtatagc gtaaataaat
     2941 ccaatgactg caatcactat cagcattaga tttttccata gaggatattt attttgcatt
     3001 tctgattccc tatgcctaac ttaaagtgat ttgatagtgc cttttggtaa tactgcagta
     3061 actgcgcctt tttgcaaatt aatttctata ccttctgctg ggcttacttt gatgtattga
     3121 tcatccaggc tcacaacttt tgccaaaatc cctccggatg ttactatttc atcccccttt
     3181 tttaagcgat ttatcaattc tctatgctct ttggctcttt tgctttgcgg ccttattatc
     3241 ataaaataaa acaagacaaa aatcgcaacg atcataatca gtgaaaaagt tccatcagtt
     3301 tgagcagctg gcgcatttcc tgctgcagct aatgcatcac taataaaaaa actcataaaa
     3361 tctcctcaaa tcgggtatta atttcctggt tttctttcga agatggtcga taaaccaatt
     3421 tcaaaagaca gttctaagaa ctcttgtatg gattaccagc gtgacatcat atagcgaatt
     3481 tactcgaagg taaatgaata gttaattacc ttgaacgtaa tacgcattat gttgaatcaa
     3541 gaatctctga catagcaatg gggtattagc taacaaatga tgcagataag ctcttgtcaa
     3601 ctgtgctgaa caagtaggac atgagcaatt ctcatcaatc agctgaaaat tgaaagcctg
     3661 gctttcatcc gttaaatcaa cattcccttc gcggccgtat acaatgcccc gcatggcatt
     3721 ttttgcaggc tcatccgatt cgattaaaat cgatttcttc ctgtttaaat cctcgattaa
     3781 atcagcaccg ataggaccag agacataaag aggtaattca gaccattttt gcaattgact
     3841 caagaaatca tcatgatatt ttgcttcact gaaattgaaa tacacaccat gctttccagg
     3901 tacctgcaag gtttcaagtt gcttcgctga caaaaaaggg aatatagtat catcccattg
     3961 ctcccatagc ccggaaaaac cattcacaag cgtttcaggt aaaagcactg cgacaggctt
     4021 aatatgcaaa atcaaatgca ctaactctga gttagttaat ttgattcgac ttccgtcata
     4081 aggtgaaatt aataccacca tcccgttttt atctgataca aggcggctcg cattgaggat
     4141 gagatgacct gaccacccta gatatttggt gaaatcagta atatttttca ataatgaata
     4201 ccctggcttt aataacaata aatccagata acaagatgcg attgtaatct taatttcttg
     4261 ccaattggcc gcagtaagac ataatcctgc ttcagtattt aaaacaggaa caaaattttg
     4321 atttgtactc aacatccact atcctcaaac tttagccacc attattatat tccagaacac
     4381 gttataacaa taaactggca tctccataac taaaaaaacg atacctttta tcgattgctt
     4441 cttgataaag agccatacac tcctggtgtc caataaaagc cgacaccaac atgaccagag
     4501 ttgattcagg taaatgaaaa ttagttatta atccatcaca aaccttaaac tgatagccag
     4561 gataaatgaa aatatcggta tcacgactac atggaattag ctctcctccc atagcagcgc
     4621 tttcaaggct gcgtaatgca gtagtcccaa cagcaattac tctatttccc atagatttgg
     4681 cagccttcac tgcagcacac agatcaggac taaccgtaaa ccactcacta tgcattttat
     4741 gatcctgaat gcgttcacag cgaacaggcc tgaatgttcc tgcccctaca tgaagagtga
     4801 cataagcaat attaactcct cgcgctctaa tactggataa aacagcatca tcaaaatgca
     4861 atcctgctgt aggcgcagca acagaccccg cgcacttggc atagacggtt tgatatctct
     4921 ccttatccag tgattcatct tctctggcaa tataaggtgg taaaggaata tgcccgagat
     4981 catttaaaag atctaaaata ttttcgctcg ccttacaaag aaataaatca tcctgcctct
     5041 ctaatacttc aagccgctta ccggcatcaa gatatatgag atcgtttgat tttaatgatt
     5101 tgctcgcttt gatatgggcc aggaaagtaa aatcgccagt tatcctctct actaacagtt
     5161 caaccttgcc gccagtggct ttgtggccgt ataagcgtgc tggtatcact ttactgtcat
     5221 tcattaccaa taagtcccct ggctgtaaga aatctgcaat ttcccggaat tgataatgac
     5281 catattcttc tgtctgtcga ttataaatta aaagccgtga atcacttcta ttcgccagag
     5341 gatattgggc tattaattcg gaaggcaaat caaaataaaa atcttgctta ttcatagaac
     5401 aacctaaaaa caaattggtg caattataca ccgaacttta gtcttaaaat caattatcag
     5461 gaatttattc attttattag aaaattaaca tcatttgttt tttatctgtt taagagacta
     5521 cgagcaagat cagcgtacag aaaattaaaa tcagggtact tgttaaatag ggataagcat
     5581 aacccttgta agttcgtttg cgatagatca ggtccagtaa taatacaggt cccactgaaa
     5641 cagtagtgag tagtataatg agtgctatcg atgtggaata tggcttaaaa ggcataatcc
     5701 gcgtcaaaaa agctaacaca gcttgcaata cttctcgaat ataataaatt acccagataa
     5761 ataaataatc aaaattataa attagccaag aagcgatgcc taaaggaaca agtaacttag
     5821 cccctttgat tgccaaaatt tttttaaagg ttctaatgaa ttcttgagag aagaaa
//
LOCUS       DAXLDT010000177         5874 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXLDT010000177 DAXLDT010000000
VERSION     DAXLDT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.177"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            35..4981
                     /locus_tag="ACF2SP_002684"
     CDS             35..4981
                     /locus_tag="ACF2SP_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HFD2360454.1"
                     /translation="MPRGNNDTASGDAAQSNLTGTLPVNFGADGAGSIDFQGMHGLSA
                     VIGNDNITYNWNASTNTLTAYQTGGALGVNDVFKIVVNPTTGQYTFTLLAAINHHAVA
                     DNTEGLVDPFVNLNYRVIDGDGDTAIGTLKVTIDDDIPKAITPEEGFVTNQAGIVRTF
                     DLDFDANIDNNVGADQLGTITFSGITNGQVVTGTVDGVPNQTLTSGGSAIHYYVSGNN
                     VVEGWINGGPGDVGSTIVFRTTLQPDMNYNASNDTYKFELFQPISTSTNVSIANFSGV
                     NATSREFIYLENASGPGEDILFSAYIRNDNGTFTDATVNTNPDGIGVNNQNMNDRENL
                     RVDFVRNASTTGTNQNMTYEYDDHYLVNNFSFKIIQVTGNPPTGSLEVWVRAYNADDD
                     DPTDNTASSANNLAHQDALRDDPQVALTQILVNGVPVTPTTVNASGGYLISGLNLNDT
                     ITIRSANGYDRVEIENPRSGAHGVSNSSLNNETFDIGLFSYNTIKTTPSEININMGLS
                     LTDSDGDKINSSIEINLAPSVFKVGENVDDTSSSNVPHRVGGDTGVIDGSGGADILVG
                     DVGGVEVVGTTARLAFILDESGSMSQNFGGTTRLEVLKQAMTDILTELSNTPNASITV
                     HLVKFASVVNGTGTFEITGGGLQQALDFISGLQIQQGLLAGTNYEAALGQTVQWFSSQ
                     SGTVDVQQTLFFTDGVPTFYMDGNSTEYTNLARVYGNGSQTEEVLWENLFGEHAGGQA
                     TSDRINNLTESDSRDLDGLQSYSIDTNNDGIFEIQSVNSRSSGTTQTTNDLVRSVADT
                     FNEVQALQAYGPLRAVSIADNANVYLQEIDSTGQPYLADSPEVLQDILDELNPFNVLL
                     AAGSDTIQANQEDDLIFGDVLFTDKLAEDEGLDLPKGSGWAVFEELEANHGWSRQDTL
                     DYIRNHADELGRETVLSSGSKRSGGHDIISGGQGDDRIYGQEGNDVIDAGSGDDVIDA
                     GSGDDVIVGGTGNDTLTGGSGADQFVFFRGHGSNNAGTAPTDIITDFEVNIDKIVINA
                     NNIIGVSVSNPVSNPDLSKTYTITVNYSDAPSIEHFKVTLSNGALLNDSGKTHLNGVV
                     ISGGTATIDGTIVGAVLYLDLNANNQEDEGERLGITNQYGHVEWVVDLSKFDVNGDGQ
                     YVIGEARAVQTGGFDIDTGLSYEINLYGPVGSAVISPLTSLLQAQLEAGMDYETANAA
                     LVARLGLPEGTQIISFNPITGSGEVLAQNAGVMTAAIQFAEIAAIHYSTDEGHVSFAV
                     FEAISKALLELPEGVVADFGDEGFLQSIANYLGLDALVTSDMIDYMATSQKALEASIL
                     TLAPGEDALTAISKIQHVTQGIYAQAIEMALIGYLPLDALKNMSAVLKAYADGDITDE
                     QLNSFEDKLSAVIVNWEDNNVTDASHQATLQTERTVDDSSVKSEANHEMAQEVNAVIN
                     DFMDEHNISLYYYDQQAQMPQETQEEQQPVEAQAEETVIHDDVVQDLLLDDHSLTMLA
                     NNPELNGGNGNDVLHGTTGNDFIRGGQGNDTMTGGGGVDTFFWLSGDDDGGVDTITDF
                     KANPVDQSSDASVLNLSDLLSDADLETNSLDNYLNVSTTEEGDTAIKVDPNGNGNFDA
                     PAQTIILEDVDLTAVFATNNSHDIVNQMIANGNLIVEQ"
     gene            5160..5759
                     /gene="wrbA"
                     /locus_tag="ACF2SP_002685"
     CDS             5160..5759
                     /gene="wrbA"
                     /locus_tag="ACF2SP_002685"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFD2360455.1"
                     /translation="MSNPYILVLYYSRTGSTAQLAQYIARGVESVSGLEARLRTVPPV
                     STTCEAVDKPVPDAGAIYVSLDDLRHCNGLALGSPTRFGNMAAPMKYFWDGTTSLWLG
                     GDLVNKPACVFSSSASMHGGQETTLISMMIPLLHHGMILLGVPYTEPALTSTQSGGTP
                     YGVTHVAGGDNENPLSQDEISLAKALGERLGRVAMGLKV"
BASE COUNT         1724 a         1018 c         1378 g         1754 t
ORIGIN      
        1 tggtaccata agcctggtag ctgatgagga caatttgcca agaggcaata atgacacagc
       61 ttcaggagat gctgctcaaa gcaatttaac tggtacattg cctgttaatt ttggtgctga
      121 tggtgcaggt agcattgatt ttcaaggtat gcatggtctg agtgctgtca taggtaatga
      181 taacattact tacaactgga atgcttctac gaatacgttg acagcctatc aaacaggagg
      241 agccttaggg gttaacgatg tgtttaaaat agtagttaat ccgacaacag gtcagtatac
      301 gtttactctg ctggctgcta ttaatcatca tgctgttgct gataataccg aaggattggt
      361 tgatccattt gtgaatctta attatcgagt tattgatggc gatggggata ctgcaattgg
      421 aactctaaaa gtaacaattg atgacgatat acctaaagca attacacctg aagaaggttt
      481 tgtaacgaat caagccggta ttgtaagaac atttgatttg gattttgatg ccaatattga
      541 taataacgtc ggagcagatc aattaggtac aattactttt tctggtatca ccaatggaca
      601 agttgttaca gggacagttg atggtgttcc gaatcagact ttaacctcag gaggatcagc
      661 tatccactat tacgtgagtg gtaataatgt ggttgagggc tggattaatg gtgggcctgg
      721 ggatgtgggt tccacaattg tgtttaggac tactttgcaa cctgatatga actataacgc
      781 tagtaatgac acctataaat tcgaattgtt ccaaccaatt agcacatcca caaatgtgag
      841 tattgcaaat tttagtggtg ttaatgcgac tagtagagag ttcatatatc ttgagaatgc
      901 cagtggtcct ggggaagaca tattattttc tgcttacata agaaatgata atggcacttt
      961 tactgatgct actgttaata ctaatcctga tggcataggt gttaataatc agaacatgaa
     1021 tgatcgtgag aatttacgtg ttgattttgt caggaacgcc tctacaactg gtacaaatca
     1081 aaatatgact tatgaatacg atgatcatta tctcgttaat aactttagtt ttaaaattat
     1141 tcaggttaca ggaaatcctc cgacaggatc actagaagta tgggttcgtg cttataatgc
     1201 ggatgatgat gatccgactg ataatacagc tagttctgca aataatttgg cccatcaaga
     1261 tgcattaaga gatgatccgc aagtagcact cacacaaatt ttagtgaatg gtgtgccagt
     1321 tactccaacc acggttaacg ctagtggtgg ctatttaatt agtggcttaa atttaaatga
     1381 tacgataaca attcgttctg cgaatggata tgaccgtgta gaaatagaaa acccacgttc
     1441 aggcgctcat ggagtttcaa attccagttt gaacaatgaa acatttgata ttggcttatt
     1501 ctcttacaat acaattaaaa ctactccttc tgaaatcaat attaacatgg gtctttcatt
     1561 gacggactca gatggcgata aaataaattc ttcaatagaa attaatctgg ctccttcagt
     1621 atttaaggtt ggagaaaatg tcgatgatac ttccagctct aacgtgcctc accgtgtagg
     1681 aggtgatacc ggtgttatcg atggatctgg tggcgcagat attttggttg gtgatgttgg
     1741 tggtgtagag gttgttggta caactgctcg acttgcgttt atactcgatg aatcaggtag
     1801 tatgagtcaa aattttggag gaacaacccg tttagaagta ctaaaacaag caatgactga
     1861 tatattaact gagctcagta atacgcctaa tgctagcata acagttcatt tggttaaatt
     1921 tgcatcagta gttaatggta caggcacttt cgaaatcact ggcggaggat tgcagcaagc
     1981 tttagacttt atatctggat tacagatcca gcaaggttta ttagccggaa caaactacga
     2041 agcagcgttg gggcaaaccg tacaatggtt tagtagtcag tctgggacag tggatgtaca
     2101 gcaaactcta ttttttactg atggagttcc tactttctat atggatggga atagtactga
     2161 atacaccaat cttgctcgtg tctatggaaa tggaagccaa accgaggaag ttctctggga
     2221 aaatctattc ggtgagcacg ctggaggtca agcaacatcg gatcgaatta ataatctcac
     2281 agagtctgat tccagagatt tggacggtct tcaaagctat tctattgaca ccaacaatga
     2341 tggtattttt gaaattcaat cagtgaattc acgaagttct ggaacaacac aaactacaaa
     2401 tgacttggta agaagtgttg cagatacttt caatgaagta caagctttgc aggcatatgg
     2461 tcctttgcga gcagtttcta tagcggataa tgctaatgtt tatctgcaag aaatagatag
     2521 tacaggtcaa ccttatttag ctgattcccc tgaagtgcta caggatatac tggatgaatt
     2581 aaatccattt aatgttttac ttgcagcggg tagtgatacc attcaggcta atcaggaaga
     2641 tgatttaatt tttggtgatg tgttattcac tgataaactg gctgaagatg aaggtcttga
     2701 tttacctaaa ggctcaggat gggcagtatt tgaagagttg gaagctaatc atggatggag
     2761 caggcaagat actttagatt atataagaaa tcatgctgat gaacttggta gagaaacagt
     2821 tctctctagt ggtagtaaac gttctggagg acatgacatc attagcggag gtcagggaga
     2881 cgacagaata tacgggcagg aaggtaatga tgtcatagat gccggttctg gggatgatgt
     2941 catagatgcc ggttctgggg atgatgtcat tgtgggtgga accggtaatg acacgctaac
     3001 tggcggatca ggagctgatc aattcgtgtt tttcagggga catggaagta ataatgcagg
     3061 aacagctcca actgatatta ttacagattt tgaagtgaat atcgataaga tcgtaattaa
     3121 tgctaataat attatcggcg tgtcagtttc taatcctgta agtaatcctg atttgagtaa
     3181 gacatatact attacagtaa attattctga tgcacctagc attgaacact ttaaagtgac
     3241 attatccaat ggtgctttat tgaatgactc tggaaagact catttgaacg gagtggttat
     3301 aagcggcggc acggctacca ttgatggcac aatcgttggt gcagttctat atttggatct
     3361 gaatgctaat aatcaggagg atgaagggga acgactgggt atcaccaatc aatatggtca
     3421 tgtggaatgg gtcgttgatc tctctaaatt cgatgtcaac ggtgatggtc agtatgttat
     3481 aggcgaggct cgtgctgttc aaaccggtgg ttttgatata gatacaggtc ttagctatga
     3541 aataaatctt tacgggccag taggttcagc agttatatcg cccttgacaa gcctcttgca
     3601 agcgcagcta gaagccggca tggattatga gacagctaat gctgccttgg ttgctcgctt
     3661 agggttacca gagggaacac aaattattag tttcaatccg attactggca gtggtgaagt
     3721 gctggctcaa aacgcaggtg taatgacagc tgccattcag tttgctgaaa tagccgcaat
     3781 acactattca accgatgaag gtcatgtttc attcgctgtc tttgaggcaa ttagcaaggc
     3841 attgcttgaa cttcctgaag gggtagtcgc tgattttggc gatgagggtt tcttgcagtc
     3901 cattgcaaat tatctgggat tagatgctct ggttacctct gatatgattg actatatggc
     3961 aaccagccag aaagcgttag aagccagtat tttgactctt gcgcccggag aagatgctct
     4021 gacggccatc agcaagatac agcatgtgac ccaaggtata tatgcccaag ctattgaaat
     4081 ggcattgata gggtatttgc cattagatgc tcttaaaaac atgagtgctg ttcttaaggc
     4141 ttatgctgat ggagatatta cggatgaaca actcaactcc tttgaagaca agctttctgc
     4201 tgtgattgtg aattgggaag ataacaatgt tacagacgca tctcatcagg ccacattgca
     4261 aacagagcgt actgttgatg actcttcagt caaatcagag gcgaatcatg aaatggcaca
     4321 agaagttaat gccgtgatta atgattttat ggatgaacat aatatttctt tgtactatta
     4381 tgatcaacag gctcaaatgc ctcaagaaac acaagaagaa caacagccag tagaagctca
     4441 ggctgaagag acggtcatac atgacgacgt tgttcaagat ctgttgcttg atgatcatag
     4501 ccttaccatg ttagctaaca atcccgaatt aaatggtggt aatggcaatg atgttctaca
     4561 tggaactacc ggaaacgatt ttataagagg cggtcaagga aatgatacga tgacaggtgg
     4621 tggcggtgtt gataccttct tctggttaag tggcgacgat gatggtggtg tggatactat
     4681 taccgatttc aaagcaaatc ctgttgatca atcatcagat gccagcgtcc ttaacttaag
     4741 tgatttgcta tctgatgcgg atctggaaac caatagtttg gataattatc tgaatgtttc
     4801 taccacagaa gagggtgaca cagccattaa agtagatcct aatggcaatg gaaattttga
     4861 cgctccagcg caaacgatta tattagaaga tgttgattta acagccgttt tcgctactaa
     4921 caattcgcat gatattgtga atcaaatgat cgctaatgga aacttgatag tagaacagta
     4981 agatgaactc attacattgg tgaatatgga attcaccaat gttttattaa ttggaaaata
     5041 tcctcgcgac atttttctcg ctgctatata ctcatgcgtt cattcgataa taagccttgc
     5101 aattcatttc acgttattaa aatgatgaaa aaaggcaaaa aacgctatgg agcagtatca
     5161 tgtctaatcc ttatatcttg gttttatact attctcgcac tggctcaacg gcacaattag
     5221 ctcaatatat tgctcgtggg gtggaaagtg tgtctgggct tgaagccagg ttacgcactg
     5281 taccccctgt atctaccact tgcgaagcgg tggataagcc ggttcccgat gctggcgcaa
     5341 tttatgtttc cttggatgat ttgcgtcatt gtaatgggtt ggctttaggc agtcctactc
     5401 gctttggtaa tatggctgct ccaatgaagt atttttggga tggaactact tctttgtggc
     5461 tgggtgggga tctggtgaac aagcctgctt gtgtgttttc ttcttcagcc agtatgcatg
     5521 gtggacagga gactacctta atctccatga tgattccttt gttacatcat ggcatgattt
     5581 tattaggtgt tccttatact gaacccgccc taaccagtac gcagtctggt ggtacgcctt
     5641 atggtgtaac ccatgtggct ggtggtgata atgagaatcc attaagtcaa gatgagattt
     5701 cgttggctaa agctctgggg gaaagattgg ggcgtgtagc aatgggactg aaggtttgat
     5761 gagagggttt taggggttga ttacccctct cccttaccct ctccccaaag gggcgaggga
     5821 atgattaatt aaactatatc cttctccaaa taggcgaggg aatgattaat taaa
//
LOCUS       DAXLDT010000178         5866 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXLDT010000178 DAXLDT010000000
VERSION     DAXLDT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.178"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..112
                     /gene="flgJ"
                     /locus_tag="ACF2SP_002686"
     CDS             <1..112
                     /gene="flgJ"
                     /locus_tag="ACF2SP_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946326.1"
                     /note="Flagellum-specific muramidase which hydrolyzes the
                     peptidoglycan layer to assemble the rod structure in the
                     periplasmic space; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFD2360456.1"
                     /translation="KAGYATDPNYSNKILSIYHGDELNQAIQRCELSEQS"
     gene            162..2111
                     /gene="flgK"
                     /locus_tag="ACF2SP_002687"
     CDS             162..2111
                     /gene="flgK"
                     /locus_tag="ACF2SP_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFD2360457.1"
                     /translation="MSILNIAYSGLNAFQRALDVTGNNIANFKTRGYSRQSIQFTPIA
                     SNRYAGSYIGAGVSVSSIYRNVDQFANAQVRSTLSYRTQYDAFYNQAIQIDKLLSQDG
                     SSISVPLQTFFDSIGQLNSTPDNIATRGVVLKQSQLLAQQFNSLQIKLEEYERNSALQ
                     VTESVKIINRITKELAEVNGKLLGNNNIPELLDHRDELLKQLSGYTDLSIFDQGDGTI
                     SVGIASGDMLVAGTQQRDLVVGTGKDSIFGTRIFLSSGGNNQVDITDRLTTGMLGGLI
                     DYEKNVLGQASQLIGQMAIGLAQTFNAQHKLGMDMNSQIGKDFFTDFNSPGQMLKRST
                     ASADNSGTAVLSVNISDISQVKLSDYDLIISDTGANELRLIRKSDGTSTTLTWSSSPP
                     APPAGQVVIDGMTITVNDLSQLANNDHYTLTPTRGAARDLALEIKDAYEIALASPVKT
                     TASLNNTGQGQIILGPVLNTASVNKQFRIDFISDTQYNLVNVTDSTTAGPFAFVPNTN
                     NVIQIPDGITPSYSVVLSGIPKSGDQFTTDYNAGGFGDNRNGLSLSNIQQNKIFSNGS
                     ETLFDRYGGLLAEVGGRTNQAKTSFESADILHKQALDFQDSKSGVNLDEEGANLLVFQ
                     QAYQAAGKLMEISNQIMNLLFDIMR"
     gene            2115..3350
                     /gene="flgL"
                     /locus_tag="ACF2SP_002688"
     CDS             2115..3350
                     /gene="flgL"
                     /locus_tag="ACF2SP_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFD2360458.1"
                     /translation="MRISTNQIYQRGLDNLLTQQERLARLQDQQASGIRVRTPSDDPV
                     ASAQIELIKQRISNIELLQNNRQAADSALRLEEGILSNTVNSLHRLREIQIQSGNPSL
                     SEEDRKTLAVEAQALLNQLLDYANTKDSNGSYMFSGSKSLTQPVSLNLSGQYVYNGDN
                     TQRFQAVTTSLLVAVNDTGDNVFMRIPSGNGRFAIRETVTPNTGTASVSSGSVINEAA
                     FVPDNYTMTFALNSQGNLVVMVSGTLSGNVIPPSGLPDDAPLYQEGSAISFNGMEMVV
                     SGLPKAGDSFSISPAKNESIFSTVQRMINNLNKPYVSSVEKAATQTENNQLLAQIDSA
                     LGRILSVQSDLGARLNQLETAEKANNDYLDISAATLKKLREIDPVAVATELNLQLVNL
                     QAAQLSFTRIQGLSLFNFL"
     gene            3545..5284
                     /locus_tag="ACF2SP_002689"
     CDS             3545..5284
                     /locus_tag="ACF2SP_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2360459.1"
                     /translation="MKTVNSQNGTSAEPSVNLGKLSTAKNLVFRGGGSKGVAYVGALQ
                     SLSEQGVLENIENVAGSSAGAMTAAIVACGGSAELMDYFCSANSLADFVDNRLTKKLL
                     PKSEKSPQAGSSVDYSSISHEQKGSQLDTESSLAEAKLVTKPSKLTFAIASKGAKLVE
                     AMEHVMRMAIFTHFPESLDNELKNVHEKLNAIEDKGSAEYINLAKRQQILEDTQKIIN
                     GLHDPNFDLTFGHLHTLHQYDPQRFKELYVTGTDENGELRIFSHEKDADMPVSIAVRI
                     SASLPGVFDPVIYEGRKYIDGGAANNLPVNIFFNKEEAYTPGVVTTIGVSCENKLDIT
                     EKEFKNPSFMKRIMGYLGDFVKKMLCKLDVAGAVKEQKREMLKYIDNGNLFVMVAPPI
                     SITTAQMHIDKEEKEGVIKVGKAFCDVQLKSMIDSDYKPQPFDEKTAKILASSGEQQE
                     QSIKKTIDIKSRLQNIKQADRPWSQHVEKTIDRLMLVKGEATQDQIDLCLQWQEIKSV
                     HSNPADRLREFAYAFLASPLAEPVSNHQEINFSKETRKEINLLKNLPGLYETTPDFTE
                     EFNNQRMSSLQRA"
     gene            complement(5409..5485)
                     /locus_tag="ACF2SP_002690"
     tRNA            complement(5409..5485)
                     /locus_tag="ACF2SP_002690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5449..5451),aa:Arg,seq:cct)
BASE COUNT         1944 a         1011 c         1233 g         1678 t
ORIGIN      
        1 taaagcaggg tatgctactg acccaaatta tagtaataag attttatcta tttatcatgg
       61 tgatgaattg aaccaggcaa tacaacgatg tgaattatcc gaacaaagtt gatagttagg
      121 tccaaggtaa agaaaaaatt agttcaggga gtgaacaaaa tatgtctata cttaatattg
      181 cctattcagg attgaatgct tttcagcgtg ccttagatgt tactggcaat aatattgcta
      241 attttaaaac cagagggtat agcagacaat ctatccaatt tacacctatt gcctcaaatc
      301 gctatgctgg ttcatacata ggagcaggag tatctgtttc atcgatttat cgaaacgttg
      361 atcaatttgc caatgctcag gttagaagta ccttgagtta tagaactcag tatgatgcgt
      421 tttataatca ggcaattcag atagataaat tgctttcaca ggatggatca agtatttcgg
      481 ttcctttgca aacttttttt gattcaatag gccagttgaa cagtacaccc gataatatag
      541 cgacaagagg tgtagtattg aaacagagtc agttattagc tcagcaattt aattcgctgc
      601 aaataaaact tgaagagtat gagcgtaatt cagcattaca agtaacagag tcagtaaaaa
      661 taattaatcg aataacaaaa gagttggctg aggtcaatgg aaaattatta gggaataaca
      721 atatacctga attacttgac catagagacg agctgctcaa acaattatct ggttataccg
      781 atttgagtat ttttgatcag ggtgatggta cgattagtgt tggaattgcc agcggagata
      841 tgctagtggc aggaacccag caacgcgatt tggtagtggg tacaggtaaa gattctattt
      901 ttggaacaag gatcttttta agtagtggag gaaataatca agttgatatt acagacaggc
      961 tgactaccgg tatgttaggc ggtcttatag attatgagaa aaatgttttg ggacaggcca
     1021 gccaattaat aggccaaatg gctattggtt tagctcagac atttaatgcc caacataagt
     1081 tgggaatgga tatgaatagc caaattggca aggatttttt cactgatttt aattcccccg
     1141 gacaaatgct gaaacgatct acagcttcag ctgataatag tgggactgct gtcttatctg
     1201 tcaatatatc agatatatcc caagtgaaat taagcgatta cgatctgata attagcgata
     1261 ctggggctaa tgaattgcga ttgataagaa aatcagacgg tacttctaca accttaacct
     1321 ggtcaagctc gccaccagcc ccaccagcag ggcaagtagt tatagatgga atgacgatta
     1381 cagtaaacga tttatcgcaa ttggcaaata atgaccatta tactctaacc cctaccagag
     1441 gagcagccag agatttggcc ttggaaatta aggatgctta tgaaattgcg ctggcttctc
     1501 cagtgaaaac aacggcgtca ttaaataata ccgggcaagg tcaaattatt ttaggaccag
     1561 tattgaatac ggcttctgtt aacaaacaat tcaggataga tttcatttct gatactcaat
     1621 acaacctggt taatgtaacg gacagtacaa cagccggtcc gtttgccttt gtaccaaata
     1681 caaacaatgt cattcaaatt cccgatggaa taactccttc ttattcagtt gttttatctg
     1741 gaattcccaa atccggagac caatttacta ctgattacaa tgctggtgga tttggtgata
     1801 atcgcaatgg cttgagcctt agtaacatac aacaaaacaa gattttttca aatggaagcg
     1861 aaaccttatt tgacagatat ggtggcttgc tcgctgaagt agggggcaga accaatcaag
     1921 ctaaaaccag ctttgaatca gccgatatac ttcataagca ggctttagat ttccaggata
     1981 gcaaaagtgg agtcaacctg gatgaggaag gagccaattt gcttgtcttt caacaagcgt
     2041 accaggcagc gggaaaattg atggaaatat ccaatcaaat tatgaacttg ttgtttgata
     2101 tcatgaggtg atgaatgcgt atttcaacca atcagatata tcagcggggt ttggataatt
     2161 tgttgactca acaagagaga ttggcaaggc tccaggatca acaagctagc ggcataagag
     2221 ttcggactcc atctgatgat ccagtggcat ctgcacaaat tgagctgatt aaacagcgca
     2281 tcagcaatat tgagctatta cagaataatc ggcaagcggc agacagtgcc ttacgtttag
     2341 aggaagggat attatccaat acagtcaatt cacttcaccg cttacgtgaa atacaaattc
     2401 agtctggaaa cccaagtctt tccgaagagg atagaaaaac cctcgctgtt gaagctcaag
     2461 ccttgttaaa tcaattactt gattatgcta acactaaaga tagtaatgga agttatatgt
     2521 tcagtgggag taaatcacta acacagccag tgtcattgaa tctctcaggg caatatgttt
     2581 ataacggaga taacacacag cgttttcaag cagttacaac gagtttactg gtagcagtta
     2641 atgataccgg tgacaacgtg tttatgcgca ttccaagtgg aaatgggcgt tttgccataa
     2701 gagaaacggt aacccctaat acaggaacag cttcagtcag ttccggttcg gtaattaatg
     2761 aagccgcttt tgtgccggat aattacacaa tgacttttgc tttaaattct cagggtaacc
     2821 tggtagtcat ggtgagtggt actctcagtg gtaatgtgat acccccaagt ggtttaccgg
     2881 atgatgcacc attatatcag gagggtagtg ccattagttt taatggtatg gaaatggtag
     2941 tgtctggctt acccaaagca ggggattcat tttcaattag tccagcaaaa aatgaatcta
     3001 ttttctctac tgtacagcgc atgataaata acttaaacaa accgtatgtc tcgtctgtag
     3061 aaaaagcggc tactcaaact gaaaataatc aattgttagc tcagattgac agtgctttag
     3121 gtcgcatttt aagcgtacag tctgatttgg gcgcaagact gaatcaactg gaaactgctg
     3181 aaaaagctaa taatgattat ctggatatca gcgctgcgac attaaaaaaa ctcagagaaa
     3241 ttgatcctgt tgctgttgca acggaattga atttacaact agtcaatctc caggcagctc
     3301 agttaagttt tacacgaatt caaggattat cattatttaa ctttttgtaa aagcatatca
     3361 aaaatggagt ttttttatct tttacatcaa gtagcattga ataatcttac tcggctcaat
     3421 cattattgta aatcaatcaa cataactata agttataacc tataaaaaga actcataagc
     3481 atgtcacatc ctgttcatat tcacttgtta ctatgtatta aatacccaat gagcaggtga
     3541 aattatgaaa acggtaaata gccaaaatgg tacatcagca gagccatctg taaatctagg
     3601 aaaattgagt actgcaaaaa atctcgtatt tcgcggagga ggctctaaag gggtagctta
     3661 tgttggagca ttgcaatcct tgtctgaaca aggggtattg gaaaacattg aaaatgttgc
     3721 aggaagctct gctggtgcta tgaccgcggc gatagtcgct tgcggtggat cagccgagtt
     3781 aatggactat ttttgtagtg caaatagttt agcggatttt gttgataacc gattgactaa
     3841 aaaattgctt cctaaaagcg agaagagtcc acaagctggc tcttctgtgg attattcttc
     3901 aataagtcat gagcaaaagg gtagccagct agatactgaa tccagtctag cagaagcaaa
     3961 acttgttacg aaaccatcca aattaacttt cgctattgca tctaaaggcg ctaaattagt
     4021 tgaagcgatg gagcatgtca tgaggatggc tattttcacg cattttcctg aatctctgga
     4081 taatgaattg aaaaatgttc acgagaagct caatgcaatt gaggataaag ggagcgctga
     4141 atatattaat cttgcaaaaa gacaacagat tctggaagac acccaaaaaa tcataaacgg
     4201 gctgcatgat ccgaattttg atttaacttt tggccattta catacgttac atcagtacga
     4261 tcctcaacga ttcaaagaat tgtatgttac tggaacagat gaaaacggtg agttgcggat
     4321 tttttctcat gagaaagatg ctgatatgcc cgtttctatt gcagttcgta tctctgcgtc
     4381 attgccagga gtttttgatc cggtaatcta tgaaggaaga aaatatattg atggtggtgc
     4441 tgctaataat ttaccagtca atatcttctt taataaggag gaagcgtata ctccgggagt
     4501 agtgactacc attggagttt cttgcgagaa taaattggac atcacagaaa aagaattcaa
     4561 aaatccatcc tttatgaaaa ggattatggg ttatttagga gattttgtta aaaaaatgtt
     4621 atgtaaactt gatgttgcag gagctgtcaa ggaacagaaa agagaaatgt tgaaatatat
     4681 agacaatgga aatctgtttg tgatggtggc gcctccaatt tccataacga ccgcccaaat
     4741 gcatatagat aaggaggaaa aagagggtgt tatcaaggta ggtaaagcgt tctgtgatgt
     4801 gcaactaaaa agtatgattg actcagatta taaaccacag ccatttgatg aaaaaacagc
     4861 aaaaatattg gcaagcagtg gtgagcaaca agagcaatcg ataaaaaaga ccattgatat
     4921 taaatcaaga ttacaaaata ttaaacaggc agacagacct tggtctcagc acgtagaaaa
     4981 aacgattgat cgactaatgt tggtcaaagg agaggccact caagatcaaa tcgatctttg
     5041 tctgcaatgg caggaaataa aatcagtaca tagcaaccct gctgatagat taagagagtt
     5101 tgcctatgct ttccttgctt ctccattggc agaaccagtc agtaatcatc aggagattaa
     5161 tttcagtaaa gaaacaagaa aagaaattaa tctactaaaa aatctgccag gattatatga
     5221 aactactcct gattttacag aagagtttaa taatcagaga atgtcatcct tacaaagggc
     5281 ataaaaagtc aatagcctgt aggatatgac ttaaagcaag aatgctttag gttacaatat
     5341 ttttaaagcc ccgatttatt gaattatata atcttggtaa ttttctttgg aatagaccct
     5401 ggtttcattg gcgtcccggg caggattcga acctgcgacc tgccccttag gagggggctg
     5461 cgctatccat ctgtgccacc gggactttga ctttatttat caaacgcaag tctaccctta
     5521 taggcgcaaa atgacaacgg gaaagcaaat ttcttatatg aaaatagtga tagggctggt
     5581 tggattggga tgtgttctta gcaggactta tgaaaaaccc ctaggtcatg gcaaaaaatg
     5641 tttttaatga gggaatttag ataaactaaa tgaccgaatt aaaacatttt ttaacataga
     5701 gattgggggt tttcgtaagc cctgcttaat tattctttca tttctcaatt ctggaaatcg
     5761 gtaagtcaat ttttattaaa cctggtgaag gttttgtgat tggctaccaa gatggatata
     5821 acctgataac agtacgacat gaaacaatat ctattgaccg cgtatt
//
LOCUS       DAXLDT010000179         5809 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXLDT010000179 DAXLDT010000000
VERSION     DAXLDT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.179"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(112..798)
                     /locus_tag="ACF2SP_002691"
     CDS             complement(112..798)
                     /locus_tag="ACF2SP_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFD2360460.1"
                     /translation="MSKFIFVIGGTGTGKTTVSEEIKTIAEAQNFTVTILSLDHYYLP
                     QSMMDPDKPKNFDVPEALEQTLIQQHLQDLEAGKTIFRPTYDMASSDRVLNGEMKIIP
                     ADIIIVEGIFAGEYLSSLRNQTEKLKIYLQSNQINDNYTRKEDRDIVERKRSSAHAHV
                     AKKNQMTGFFRYVATHMISSNMVIDNPWLPQQNMTTTEKLPLIAGNNLIKLNEFLSST
                     ESHFLYKTYS"
     gene            complement(1154..2308)
                     /gene="argE"
                     /locus_tag="ACF2SP_002692"
     CDS             complement(1154..2308)
                     /gene="argE"
                     /locus_tag="ACF2SP_002692"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFD2360461.1"
                     /translation="MTTLDWLKQLISFDTTSRNSNLSLIEYLANWLNDFKVNPILIHD
                     SKEQKANLLATLPGKQGRLEGGIILSGHTDVVPVDGQIWDSDPFQATLKNNKVYGRGA
                     CDMKGFIAVVMALVPQLKEMNLDFPVHFAFSYDEEIGCLGVPSLIDKIVELNYQPRAC
                     IVGEPTLMKPVVGHKGKYSYRCQIHGVAAHSSLTNQGSNAIEHAASFISYLRGMANEF
                     KKYGNRDFSYDVPYTTLTTNLIKGGNAYNTIPNLCEFVFEFRNLSTDSSEDLNQKIMS
                     YVKDQLVPNLHHDHPDSNVNLDTIAKAPGLDMPITDPFVRAAQTVCQSDKLMKVAYAT
                     EAGLFQQAEIPTIVCGPGSIEQAHRANEYVEVEQLRLCEEFIINMLRSHF"
     gene            complement(2320..3606)
                     /locus_tag="ACF2SP_002693"
     CDS             complement(2320..3606)
                     /locus_tag="ACF2SP_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360462.1"
                     /translation="MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPDHS
                     PVTSLLATMASSGVALVVRPLGGFILAVWADKYGHHKALMFVFYLMAVATMLIAFTPS
                     YSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
                     LMGSSVCLILTLYCSNETLYQFAWRIPFALGLLIIPLGWILRRRLQREFTWKKEQQDT
                     GKLLIMVKQQKRSLLIAFSLVAGCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGI
                     LVSILTIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQYPSVTILILVENILG
                     LCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMV
                     FTYYQMTTVFISLLAVLSYQEERKLSENQPEHLVAI"
     gene            3777..4508
                     /locus_tag="ACF2SP_002694"
     CDS             3777..4508
                     /locus_tag="ACF2SP_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFD2360463.1"
                     /translation="MKKFILFPLISALSSTFVHAGGMGDSNSCCSTFVSLEGGYTWSS
                     IDGYNFTIVGTNNTLTSTEDKQGYSGRLAVGVLSMIDDQYGFTGEVGWGYYGRTTINP
                     SVAGALGQVPAALTIKHTLSGFDALLGVTYFQNYFSWSAKAGALIQNMQVDTSAFFTP
                     QIFPIVDNFDMKTNRTAVLPAAKLGVAYNFDSNWAITGSYLFALGANPGTTATFNPNT
                     LRASLSIDDENPMMNAILFGVQYTA"
     gene            complement(4575..5225)
                     /locus_tag="ACF2SP_002695"
     CDS             complement(4575..5225)
                     /locus_tag="ACF2SP_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFD2360464.1"
                     /translation="MDRFQDRYQAGQLLGAKLKPYANRNDVIVLALPRGGVPVAAMIS
                     EAINAPLDLMIVRKLGVPGHEELAFGALAINKIKVYNEELLQYLQLSQKDINQVLSQE
                     ENELARRNRAYRGDKPFPDLKNKIVILVDDGIATGASMRAAVLALRKESPKKIIVAVP
                     VAPEDMNDIMKSVADEYICLISPMSFNAVGLWYEDFSQTEDEEVLYLLGKVYDRKE"
     gene            5557..5802
                     /locus_tag="ACF2SP_002696"
     CDS             5557..5802
                     /locus_tag="ACF2SP_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360465.1"
                     /translation="MNVHTMCFTRVLRYAVVTSLLFMVVSFTSVANAAQGCGEGYHRA
                     IHNGTCVLNYPGAFATPAPAHPGCWRNMWGQLRCYRY"
BASE COUNT         1888 a         1129 c         1013 g         1779 t
ORIGIN      
        1 tctatgaaga gtattgtaat cctaacaagg aatcactgag cttttaactc aattttaaat
       61 attgcatttt atattgcccc taaattatct actggggcaa taagttacat tttaactata
      121 tgttttatat aaaaaatgac tctcagttga agataaaaat tcatttaatt ttattaagtt
      181 attgcctgca atcaaaggaa gtttttcggt agtagtcatg ttttgttgag gaagccaagg
      241 attatcaatg accatatttg aagaaatcat atgggtagcg acatatctaa aaaatcctgt
      301 catttgattc tttttcgcaa catgggcatg agctgatgat ctttttcttt caactatatc
      361 tctgtcttcc ttccttgtgt aattgtcatt tatttgattg gattgtaaat aaattttgag
      421 tttttctgtt tgattcctta aagaggataa atactcacca gcaaaaattc cttcgacaat
      481 aatgatatca gcaggaataa ttttcatttc cccattcaat accctgtcac tgcttgccat
      541 atcataggtc ggtctgaata tggttttacc agcctctaaa tcctgcaaat gctgctgaat
      601 taatgtttgt tccagagctt cagggacatc aaaattcttt ggtttgtccg gatccatcat
      661 actttggggc aagtaataat gatccagact taatatcgta accgtgaaat tttgtgcttc
      721 tgctattgtc ttaatttctt ctgaaacagt cgtttttcca gttccagttc ctccaatgac
      781 aaagataaat ttagacacgt aatattctct tgcagtcaaa aaaggcatta taataactga
      841 ggaacaaatt acaagagaac ctatcaatct gtaccttgat aagctctcga gccaaatcca
      901 gtactccaaa gcttacaaag caaaccattt taaagattgt agataaaata aaatgattac
      961 ggcgaatgct ggcataaaaa acatttattt taataaccaa ttgataaaaa aatatttttc
     1021 tttaaaacca atcaaatttc atctgatcat gttatccaca aaaactgtgt atatctttgt
     1081 gaatacctta taaaagtgct agtaaaatca gcatcaaata ccgatgattt tttacttacc
     1141 aatctgataa ggctcaaaaa tgagagcgca gcatattgat tataaattcc tcacataatc
     1201 gtaattgctc tacttcaaca tattcatttg ctctatgggc ttgttcgata ctacctggac
     1261 cacacacaat agtaggaatt tctgcctgct gaaatagccc tgcttctgta gcataagcta
     1321 ctttcatcag cttatcgctc tgacaaactg tttgagctgc tctgacaaaa ggatctgtaa
     1381 tcggcatatc caaaccagga gcttttgcta tggtatcaag attaacatta gagtcaggat
     1441 gatcatgatg caggttagga acaagctgat ccttaacata agacataatt ttctgattta
     1501 aatcctcgga gctatcagta gacagatttc taaattcaaa aacaaactca cacaagtttg
     1561 gaatagtatt ataagcatta cctcctttga tcagattggt ggtaagtgtt gtatatggaa
     1621 catcataaga aaaatctcga tttccatatt ttttaaattc attagccatc cctcgaagat
     1681 agcttataaa agacgcggca tgttcgatcg cattagatcc ttgattcgtt aatgaagagt
     1741 gggcagctac accatgtatt tggcagcgat aagaatattt gcctttatga cccacaacag
     1801 gcttcattaa ggttggctca cctacaatgc aagcacgcgg ttgataatta agctcaacta
     1861 ttttatcgat cagagaaggc acaccaagac aaccaatttc ctcatcataa gaaaatgcaa
     1921 aatgaactgg aaagtccagg ttcatttctt tcaattgtgg gactaaagcc atcacgactg
     1981 caataaatcc tttcatgtca cacgcgccac gaccatacac tttgttattt ttaagagtgg
     2041 cttgaaaagg atcactatcc caaatttgac cgtcaacagg aacaacatca gtatgaccag
     2101 acagaataat acccccttcc aaacgccctt gttttcctgg caaagtagct aacagattag
     2161 ctttttgctc tttactatcg tgaattagga taggattaac cttgaaatca tttaaccaat
     2221 tcgccaaata ttcaatcaat gataaattag agttccgcga agtagtatca aaagaaatga
     2281 gttgttttaa ccaatctaaa gttgtcatat tcctaccttt taaattgcaa cgagatgttc
     2341 tggctgattc tcagatagct tcctttcctc ctgataagaa aggactgcta gcaaactgat
     2401 aaagactgtc gtcatttgat aataagtaaa aaccataggg tttttagtgt attcaatcaa
     2461 cgctgttata ataaatggag caaaaccacc aaacaacagg acagctgaat tatagctgac
     2521 ggctaaacaa gtagaacgta ctgatggtgg aaataaggag ctaactgtaa tggttaacac
     2581 tccaaaataa gcgccgatac acaaacctaa aatgttttca accaaaatga gaatagttac
     2641 actagggtat tggttgagca agaaaagtaa aggataaatt aatccaaaat aacaagccaa
     2701 agcacataac ataactattt ttttgcctat tctatccgac aatgctccaa aaaagggaat
     2761 agtaagaata ctgactaata ttccagatgc agtgcctaag tacgcctctg ttagcgataa
     2821 attcaagtac aatttacaat aggtaggcat atagctgaat aaggtataaa tcgagcctgt
     2881 acacccggct acaagactaa aagctatcaa cagcgaacgt ttttgttgct ttaccatgat
     2941 aagtagcttt cctgtgtcct gttgctcttt cttccaagta aattcccgtt gcaatcgacg
     3001 tcttaaaatc catcctaaag gtataatcaa tagtcctaaa gcaaaaggta ttctccaggc
     3061 aaattgatat agggtttcat tggaacaata gagagttaaa ataaggcaca ctgaagatcc
     3121 cattaatatt gccaaaagct ggcctaccat ttgtaaggaa gtgtaaaacc cctttttatc
     3181 gcttggcgat aagctaacta atagagcgct ggatatgcca aactcgccac cagtagcaag
     3241 cccttgcaac aatctcgcaa atacaatgac acaagtagca gccagaccta ttgtggagta
     3301 ggatggtgta aaagcaataa gcatagttgc aacagccatc aaataaaaaa cgaacattaa
     3361 tgctttatga tgcccatact tatctgccca aactgctaaa ataaaaccac caagaggccg
     3421 gactaccaga gccactccgg agcttgccat tgtagccaag agggaagtca caggactatg
     3481 atcgggaaaa aaagcttggg caataaaaag cagcatgaag ttataaatca tgaaatcata
     3541 ccattccata gcattaccaa tacatattgc tcccaatgtt ttaaaggagg tatttgatgt
     3601 attcatgttt ccttacaagg gtaaatttag agcaaagcat atactagtag aatataaaaa
     3661 aaattaccat gctttataag aaactaatat caatattaat tttactggta attccctatc
     3721 aactgattta aattattgac ttttacaata ataaacatgg aatgagttac taaatcatga
     3781 aaaaatttat cctgttccct ttaatttccg ccctgtccag tacttttgtc catgctggtg
     3841 gtatgggtga ttccaactct tgttgctcta cttttgtgtc acttgagggt ggatacacct
     3901 ggagttccat tgatggatac aattttacta tagtcggtac caacaatact ctcacctcga
     3961 cagaagataa acaaggttat agtggccgtc tggctgtcgg tgtgcttagt atgatagatg
     4021 atcaatatgg attcacaggc gaagtaggct ggggttacta tggtagaacg accattaatc
     4081 caagtgttgc aggagcttta ggacaggttc cagctgcctt gacgatcaag cataccttga
     4141 gcggatttga tgccctgctc ggagttacct attttcaaaa ctattttagc tggtctgcca
     4201 aggcgggcgc cctcattcaa aatatgcaag tagacactag tgcctttttc actccccaaa
     4261 tatttcctat agtggataat ttcgatatga aaacgaacag gactgctgtt ttgccagcag
     4321 cgaagctggg agttgcctat aactttgatt ctaactgggc tattaccggc tcttatttgt
     4381 ttgcgcttgg agcaaatcca ggaacaactg caacttttaa tcctaacaca ttaagagcca
     4441 gtctgagcat tgacgatgaa aatcccatga tgaatgctat tttatttggg gttcaatata
     4501 ccgcttagtt taatattatt taaaaacgtc accttttata tggggtcaag tatttggccc
     4561 caagactttt taacttattc ctttctatcg tatacttttc caagtaaata taatacttct
     4621 tcatcctcag tctgggagaa atcttcatac caaagaccca cagcgttaaa agacatgggg
     4681 gatataagac aaatgtattc atccgcaaca cttttcatta tgtcattcat atcttcaggt
     4741 gctacaggaa ccgctactat aattttttta ggtgactcct tacgtaaagc aagaacagct
     4801 gcacgcatac tagcgcctgt ggcaataccg tcatctacca gaattacgat tttatttttt
     4861 aaatcaggaa aaggtttgtc accacgataa gctctattcc gccttgctaa ttcgttttct
     4921 tcttgagaaa gaacctgatt aatgtctttt tgagaaagtt gcaaatattg caacagctct
     4981 tcattataaa cttttatttt attaatggcc aaagcaccga aagctaattc ttcatgccca
     5041 ggaacaccca gtttgcgaac aatcattaaa tctaatggag catttatagc ctcagaaatc
     5101 attgcagcta caggcactcc tccacggggt agtgcaagca ctatgacatc attccggttc
     5161 gcgtaaggct taagctttgc tcctaataat tgtccagctt gataacgatc ttgaaagcga
     5221 tccattttcc tactcaatta tctgtcctgc tacttttatt gtagttcaga tatggaaatt
     5281 atcctgctgt cagcaagagt ttataatgat taatcttctc tgaatttaag ctaataatta
     5341 tcagtacact atttcatcat ttgaatccat ttcaaataaa gtgaatagct gtgtacatca
     5401 agatagtgca tcaaggaata taaagaatga attaatttat agagtttaaa gatcaccaac
     5461 caaaaaatat gatcatattt taggcataat ttaaaaattg actctatact tatgctgtat
     5521 gctaaaaaat aattgaagca atataaggag agtacaatga atgtgcatac catgtgtttt
     5581 acgagggtgt tacgttatgc tgttgtgaca agccttctct ttatggttgt gtcttttaca
     5641 tcagtggcaa atgcggctca gggatgcggg gaaggttatc acagggccat tcataatggt
     5701 acttgtgttc taaactatcc aggcgccttt gcaacccctg caccagcaca tccaggctgc
     5761 tggcgtaata tgtggggaca attgcgttgc tacagatact aattcaaac
//
LOCUS       DAXLDT010000180         5780 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXLDT010000180 DAXLDT010000000
VERSION     DAXLDT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.180"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            32..1330
                     /locus_tag="ACF2SP_002697"
     CDS             32..1330
                     /locus_tag="ACF2SP_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360466.1"
                     /translation="MAHYNVQSESLNKASVSLIMPWMVWGLGCLFYFYECLLQVSPSV
                     MSNELMRDFSVTSHTLGILSGIYFYSYAAMQLPGGVLMDYFGPQRLLTLATVVCAVST
                     IAFGMTDNFFMACVARLMIGFGSAFAAVGTMKLAANWFPAQKFALLTGLMVTIGMMGA
                     IGGETPLALLIDNFGWRESMIIMGSVGLLLATLLILIARDTPKNFSPAIHQHPVEEER
                     LIPSLFTLIKNKQLWLVATYGGLMYMATPVFCGLWGVPFLMNKMLIEKSTAANFISLV
                     FVGWAIASPLWGLFSNRIGLRKPPMYIGCVGAMICSLLFIFAPITSSITMEILLFAFG
                     IFSAGFLPAFSVAKELCNKKYVATGLSFMNMMNMIGIALAQPLIGYILDKMWQGQMMG
                     TVRVYPLEAYYTALSILPIGMLVALLILPKIKETYCQSVN"
     gene            1367..2710
                     /gene="miaB"
                     /locus_tag="ACF2SP_002698"
     CDS             1367..2710
                     /gene="miaB"
                     /locus_tag="ACF2SP_002698"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFD2360467.1"
                     /translation="MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVI
                     LLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIV
                     FGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADL
                     ALLIHYIAAIDGIGRIRFTTSHPLAFSENLINAYAEVPELANHLHLPVQSGSDRILSL
                     MKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSF
                     SFIYSPRPGTPAANLPDDTPMEIKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGF
                     SKKSSQQLSGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA"
     gene            2710..3675
                     /gene="rluD"
                     /locus_tag="ACF2SP_002699"
     CDS             2710..3675
                     /gene="rluD"
                     /locus_tag="ACF2SP_002699"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFD2360468.1"
                     /translation="MIEHIQQQLSVPRELHNQRVDSVLARLLPEYSRSLICNWIKTGA
                     VTLNNLPCKPKDKALGGDLITINVDHTIVNTDFNLCKPENIPIQIIYEDSEVIVVNKP
                     AGLVVHPGAGNKEHTLVNALLHHCPELQLLPRAGIIHRLDKDTTGLLIVAKNLISHTS
                     LIRQMQNREIKRHYLTLVHGYVISGGTIDTGFGRHPKNRLKMAVTDFGRQAVTHYNIN
                     KHYKDFTLLNVSLMTGRTHQIRVHMNYINHPVVGDQLYGGRMKFPPNADEAILSVLKQ
                     FKRQALHACSISFCHPVKNTEITLNALLPDDFELLLNTLDQHDEK"
     gene            3665..4390
                     /gene="pgeF"
                     /locus_tag="ACF2SP_002700"
     CDS             3665..4390
                     /gene="pgeF"
                     /locus_tag="ACF2SP_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFD2360469.1"
                     /translation="MKNRFANWPAPENITALSTTRLYGFSDGPYKSNNLGLHVGDNQQ
                     HVLKNRQQLIELIDLPAEPIWLEQTHSTKCITVETEKTRDADAAITRLPKLPLVIMTA
                     DCLPILLCNRQGTEIAAIHAGWKGLYNGIIENTLKKLNSNRTDIIAWIGPAICKKCYE
                     VGEEVYLSFTNKYSLTRNAFEPVHSKWLADLPQIAEIVLNLEGVKSVFQSNLCTFELK
                     NEFYSYRRESQTGRIGTFIWINE"
     gene            4407..>5780
                     /locus_tag="ACF2SP_002701"
     CDS             4407..>5780
                     /locus_tag="ACF2SP_002701"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HFD2360470.1"
                     /translation="MITRIKLFIASLLVFFIPVADAAQDLTNMPSLAPVLKNAMPAIV
                     NVAVQGYLPNNMASGNADDDDGENSKQPSRIPEKGRKFESIGSGVIIDPKNGIIITND
                     HVIRNANLITITLQDGRRLKARLIGGDSETDLAVLKIDAKNLKSLVIGDSDKLEVGDY
                     VVAIGNPFGLNSFGNSQSATFGIVSALKRSDLNIEGVENFIQTDAAINPGNSGGALVN
                     AKGELIGINTAIISPYGGNVGIGFAIPINMVKDVAQQIIKFGSIHRGLMGIFVQHLTP
                     ELAQSMGYAEDFQGALVSQVNENSPAQLAGLKSGDVIVQINDTKITQATQVKTTISLL
                     RAGSTAKIKILRDNKPLTLDVEVTDIKKHEQKLQSNNPFLYGLALRNFEQESPPHGNV
                     VGVQVVGASETSAGWRAGLRPGDIIISANKTPVKDIKSLQAVAHDKKKQLLVQVLRGA
                     GALYLLII"
BASE COUNT         1804 a         1076 c         1188 g         1712 t
ORIGIN      
        1 tgattggata aatttggaga atttttaaat aatggctcat tacaatgtac aatccgaatc
       61 gctcaataaa gcaagtgtca gcttaattat gccctggatg gtgtggggct tgggttgctt
      121 attctatttt tatgaatgct tattgcaagt atccccaagc gtaatgagta atgaactcat
      181 gagagatttc tcggtaacaa gtcatacttt aggcattcta tcgggcattt atttctattc
      241 ttatgccgcc atgcagcttc ctggtggtgt tctaatggat tattttggcc cacaacgttt
      301 attaactctt gccacagtgg tttgtgctgt gagcactata gcttttggca tgacagataa
      361 tttcttcatg gcctgtgtcg caaggctaat gattggattt ggttccgcat ttgctgctgt
      421 tggcaccatg aaattggcag ccaactggtt tccagcacaa aaattcgcat tattgactgg
      481 cttgatggtt accataggca tgatgggtgc aataggtgga gaaacacctt tggctcttct
      541 aattgataat tttggctggc gagaaagcat gattataatg ggtagtgtcg ggcttctttt
      601 ggcaactttg ctcatcttga ttgccagaga tactcccaag aatttttctc ctgctatcca
      661 tcaacatcct gtcgaggaag aacgtttaat cccaagttta ttcacattga taaaaaataa
      721 acaactttgg ctggttgcta cctatggcgg gttaatgtac atggcaacac ctgtattttg
      781 cgggctttgg ggggttcctt ttctgatgaa taaaatgcta atagaaaaat ctacagcagc
      841 taattttatc tcattggtat ttgtgggttg ggcaatagcc agtcctttat ggggtctgtt
      901 ttcaaaccgt attggattac gtaagccgcc tatgtatatt ggatgtgtag gggcaatgat
      961 ttgttctttg ctctttattt ttgctcccat cacttcttca attactatgg aaatcctgct
     1021 gtttgcattt ggtatctttt ccgcaggctt tttgccagct ttctcagtag ccaaagaatt
     1081 atgtaataaa aaatatgtgg caacagggct tagtttcatg aatatgatga atatgattgg
     1141 aatagcgtta gcacaacctt taattggtta tattctggat aaaatgtggc aaggtcaaat
     1201 gatggggact gtgagagttt atccattgga agcttactac actgctttgt caatccttcc
     1261 aataggcatg ttagttgcgt tactaatatt accgaaaatc aaagaaacct attgccaaag
     1321 tgtcaattaa tgaaataata ccgcaatttc aatttgtaga atcaaaatgg ttaaaaaatt
     1381 atatattaaa acaaatggct gccaaatgaa tgaatacgat tcttctaaaa tggcagaagt
     1441 actttacgct tcccatggtt tggtcaaaac tgatcaagtc gaggatgccg atgttatttt
     1501 attaaacaca tgctcaattc gagaaaaagc acaggaaaaa gtattttctc aactgggtca
     1561 atggcgggaa tataaagcca aaaaccctca tgtgctcatt ggagtgggag gatgtgtagc
     1621 gagccaggaa ggctcagaca tcataaaaag agcaccgttt gttgatatcg tctttgggcc
     1681 acaaacattg cataggctcc ctgccctgct caatgaaaga cttgaaaaaa ataaatccgt
     1741 tgttgatatc agctttccag aaatagagaa atttgatcat ttacccgcac caagagcaga
     1801 aggccctaca gcttttgtat ctataatgga aggctgtagt aaatattgca gcttctgtgt
     1861 agtgccctac acgcgtggtg aagaaatcag cagaccattt gatgatgtat tggctgagtg
     1921 ttatcaatta gccagtcaag gcgtcagaga gattaattta ctaggtcaaa atgtgaacga
     1981 ttacagaggt attatggata atggggatat agccgatctt gccctgttaa ttcattatat
     2041 tgccgctatc gatggaatag gaagaatacg tttcacaaca tcacatccct tggcattttc
     2101 tgaaaatttg attaatgcct acgctgaggt tccagagctt gccaatcatt tacaccttcc
     2161 ggtacaaagc ggatccgatc gcattttatc gttaatgaaa agagggtaca ctgctttgga
     2221 atttaaatcc aaaattcgta aattaaggaa aattcgacca gacattcgct tatctactga
     2281 cattattgta ggctttcccg gagagacaga caaagatttc caggacacta tggatcttgt
     2341 acatgaaatc ggttttgata cctctttcag ttttatatac agcccaagac caggaactcc
     2401 tgcagcaaat ttacctgatg acacgcctat ggagatcaaa aagcaacgtt tacagatttt
     2461 acaaaacaga ttattaatga atgcagctcg ttacagtgaa tcaatgatag gcagcaagca
     2521 aaaaattctg gttaccggat tttccaagaa aagttcacag caattatctg gtcgtactga
     2581 atgtaaccgt gtagttaatt ttgatggacc acctcattta attggacaat ttatagatgt
     2641 ccaaattagt gacgcactac ctaattcatt gcgaggaagg ctgcttgaaa aggagatgca
     2701 gccagcctaa tgattgagca tatccagcaa caattatcag tacctcgtga attacataac
     2761 caacgagtag atagcgtgct tgccaggctg ctccctgagt attcacgctc tcttatctgt
     2821 aactggatta aaacaggtgc tgttaccctt aataatctgc cttgcaaacc caaagataaa
     2881 gcgctgggcg gtgatttaat tacgattaat gttgaccata caatagttaa tacagatttt
     2941 aacttgtgta aacctgagaa cattccaatt cagataatct atgaagacag tgaagtaatt
     3001 gttgttaaca agccagccgg tcttgttgta catccgggtg cgggaaacaa ggaacataca
     3061 ctcgttaatg cgctgcttca tcattgtccc gagttgcaac tacttcctcg tgctggaatt
     3121 attcacagat tggataaaga taccactggt ctgctgatag tggctaaaaa cctgattagc
     3181 catacttctc tcatcaggca aatgcaaaat cgcgaaatta agcgccacta tctgacatta
     3241 gttcatggtt atgtcatttc tggtgggaca atagatacgg gttttggcag gcatcccaaa
     3301 aatagactaa agatggctgt aactgacttc gggcgtcaag ctgttaccca ttataatatc
     3361 aataagcatt ataaagactt taccttactg aatgttagct taatgacagg acggactcat
     3421 cagataagag tgcatatgaa ctatattaac cacccggttg taggagatca actgtatgga
     3481 ggaagaatga agtttcctcc taatgctgat gaggcaatac tctcagtttt aaaacaattt
     3541 aaaagacaag cacttcatgc ctgttccatt tcattttgtc atcctgtaaa aaatacagaa
     3601 ataacactga atgcattatt acccgatgat ttcgaactct tgttaaacac tctggatcaa
     3661 cacgatgaaa aatagatttg ccaattggcc ggctcctgaa aatattaccg ctttgagtac
     3721 tacccgtttg tatggtttca gtgacggacc ctataagagc aacaaccttg ggttgcatgt
     3781 tggtgataac caacagcatg tactcaaaaa cagacaacag ctcattgaac ttattgatct
     3841 gcctgcagaa ccaatctggc tggaacaaac acatagcaca aaatgcatca cggtagaaac
     3901 tgaaaaaacc agagatgctg atgctgcaat tactcgttta cctaaactcc ctttagtcat
     3961 tatgacagca gattgcctgc ccatattgct atgcaatcga cagggaactg aaattgccgc
     4021 cattcatgct ggatggaaag ggctgtataa tggtattatt gaaaatacac taaagaagct
     4081 aaatagcaat aggacagata taattgcctg gataggccct gccatctgta aaaaatgtta
     4141 tgaagtgggt gaagaagttt atctgtcttt taccaataaa tactccttaa ccaggaatgc
     4201 gtttgagcct gttcatagca aatggttagc tgatttacct caaattgcag aaatagtact
     4261 taatttggaa ggggtaaaat cagtatttca gtcaaatttg tgtacttttg agttaaaaaa
     4321 tgaattctat tcctatcgca gagaatcaca aacaggtaga ataggtactt tcatctggat
     4381 taatgagtaa aatcaggatt aataatatga ttacacgtat aaaattattc atagcaagtc
     4441 tgctggtatt tttcatcccg gtagccgatg cagcacaaga tcttacaaac atgcccagcc
     4501 tggctcctgt tctgaaaaat gctatgccgg ctattgttaa cgtggctgtt caaggctact
     4561 tacctaataa tatggcatcc ggaaatgcgg acgatgatga cggggaaaac agtaaacagc
     4621 cttccagaat acctgaaaaa ggcagaaaat ttgaaagtat agggtccgga gttatcattg
     4681 accctaaaaa tggcattatt ataacgaatg accatgttat tcgtaacgcc aatttaatta
     4741 ctataactct tcaagatggc aggagattaa aagctcgcct gattggaggt gatagtgaaa
     4801 cagacttggc tgttttaaaa attgatgcta aaaatttgaa atccctggta attggtgatt
     4861 ccgataagct ggaagtcgga gattatgttg ttgctattgg aaaccccttt ggattaaaca
     4921 gctttggaaa tagtcaatct gctacgtttg gaatagtgag tgccttaaaa cgcagcgatt
     4981 taaatattga aggtgttgaa aactttattc aaactgacgc agctatcaat cctggtaatt
     5041 caggaggtgc tttggtcaat gcaaaaggcg aattaattgg cattaataca gccattattt
     5101 caccctatgg aggaaatgta ggtattggtt ttgcgatccc aattaatatg gtaaaagatg
     5161 tagcgcagca aatcattaaa tttggctcta ttcatcgtgg tttaatgggt atatttgttc
     5221 aacatttaac accagaactt gcccaatcaa tgggatatgc cgaagatttt caaggagctt
     5281 tagtatcaca ggtcaatgaa aattcacctg ctcaattggc tggtctaaaa tcaggcgatg
     5341 tcattgtaca aattaatgac accaagataa ctcaggcaac acaggtaaaa acaactatta
     5401 gcctgttgcg agccggctct actgctaaaa ttaaaatctt gcgggataat aagccgctta
     5461 cattagatgt agaagtcaca gatatcaaaa aacatgaaca aaaattacaa tccaataatc
     5521 catttctcta cggattagcc ttacgtaatt ttgaacaaga atcaccgcct catggtaatg
     5581 ttgttggagt tcaggttgta ggtgcttcgg aaaccagtgc gggctggcga gctggcttaa
     5641 gaccaggaga tataatcatt tctgctaata aaacaccggt taaagacatt aaatctttac
     5701 aagctgttgc acacgacaaa aagaaacagc tattagtcca ggtgctgaga ggagcaggag
     5761 cactctacct gttgattatt
//
LOCUS       DAXLDT010000181         5770 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXLDT010000181 DAXLDT010000000
VERSION     DAXLDT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.181"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            186..1004
                     /gene="phhA"
                     /locus_tag="ACF2SP_002702"
     CDS             186..1004
                     /gene="phhA"
                     /locus_tag="ACF2SP_002702"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HFD2360471.1"
                     /translation="MEFSSRYVAHVPDAQGLVDYSAQENRIWNILFERQLKLLPGRAC
                     DEFLSGLQTLGLNSSTIPQLPEVSERLKAKTGWQVAPVAALISAREFFELLAEKYFPA
                     ATFIRSEEELDYVQEPDIFHELFGHCPMLTDRVYAEFVHDYACKVLTFPEQDWPLLQR
                     MFWFTVEFGLIKTPKGLRAYGGGILSSISETVYCVESDIPVRILFDPVVAFRMPYRID
                     QLQPVYFVIDSYQNLYDFVLSDMGKFMDRARELGEFPPYFDVDPENPNIHIRAC"
     gene            1129..1788
                     /gene="letA"
                     /locus_tag="ACF2SP_002703"
     CDS             1129..1788
                     /gene="letA"
                     /locus_tag="ACF2SP_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator LetA"
                     /protein_id="HFD2360472.1"
                     /translation="MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVK
                     THSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYL
                     TKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQEPQDSPFDLLSEREMQVMLMI
                     TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQD
                     D"
     gene            1800..3656
                     /gene="uvrC"
                     /locus_tag="ACF2SP_002704"
     CDS             1800..3656
                     /gene="uvrC"
                     /locus_tag="ACF2SP_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFD2360473.1"
                     /translation="MNDLQLSAELALFLTKLPSEPGIYRMLDEEGTVLYVGKAANLKK
                     RVNSYFSKQNTGVKTRALVSQIKSIEISVTRSETEALLLESNLIKALRPKYNVLLRDD
                     KSYPYIHLSNHPDFPRIELYRSKKKPPSGNFFGPYPGVAAVRETIVTIQKIFKIRNCR
                     DSYFKARSRPCLQYQIKRCTAPCVHYISPENYKLSVEDAIRFLQGKCQIILDALAERM
                     KQAVNQLNFEEAAVLRDQIKNLRLIQEQQGVVQLRGDADVIAIEVRPGFACIQCVTIR
                     EGQVLNSQSFFPTVPYAVLDEELDANSLWQQTFEAFIGFYYLDTTERIPDLIITNQSI
                     TESRSLEYILSQRRGKSCKIQVNPRGVKSRWMDFAVNNLRISVAEYVSKHSTIRSRYQ
                     ALKQLLALDKNIERMECFDISHTQGEATVASCVVFDTEGPRPSEYRRFNIEGITPGDD
                     YAAMEQAVTRRFKRLIDAQLLPDVLIIDGGKGQVSIVKRVLTSLGVEDITLLGVSKGP
                     SRKAGWEKLILVNENREFVLPEDSKALHLLQHIRDEAHRFAITAHRKKRQKTRVESTL
                     ESIEGVGAKRRQALLQRFGGLRELAKAPLEEICKVQGISEQLAKRIHEHFHP"
     gene            complement(3680..4828)
                     /gene="lcl"
                     /locus_tag="ACF2SP_002705"
     CDS             complement(3680..4828)
                     /gene="lcl"
                     /locus_tag="ACF2SP_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like adhesin Lcl"
                     /protein_id="HFD2360474.1"
                     /translation="MIHRNKVLNSILLILFISQIAFAKSNPASQAYVDGKVSELKNEL
                     TNKINSIPSGPQGLPGPKGDRGEAGPQGLPGPKGDQGEAGPQGLPGPKGDQGEAGPQG
                     LPGPKGDRGEAGPQGLPGPKGDQGEAGPQGLPGPKGDRGEAGPQGLPGPKGDRGEAGP
                     QGLPGPKGDKGETGAVGPQGMPGPKGDQGEAGPQGLPGPKGDKGETGAVGPQGMPGPK
                     GEAGDDGQGVPAGGETGQILAKSNDLDFNTMWVDPANSGIRRQLGDKALGGTVIYVNA
                     LGTHGLVVANSDQVNSSTWWDAQDSITNPAHFDNEGKLYSDWRLPSRFELNLIYMMRD
                     ELGNFLAGNYWSSIEKSSANSWVFNSKTGEIKDIAKNKTAAVRAVRAF"
     gene            complement(4996..5655)
                     /locus_tag="ACF2SP_002706"
     CDS             complement(4996..5655)
                     /locus_tag="ACF2SP_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360475.1"
                     /translation="MPFCFSNQPNILNNKLILKILVLILFFSNSSLAKTINYPWTVSA
                     SLGYVNYQYVHEGDHITATGRFAIGRDLLMKGPFLFGLELGLQSGNQMRLQMTEEEVD
                     ALGGLLVQTTLKPMVDALATIRITSEFTAPLFLQLKAGTAYRQWQMDRDTINDISQLA
                     GEIMAGCGYTITPNASITLSYQGLFGDKPNFTIDLESGIGHVQKIPVQHAILLGIVLM
                     I"
BASE COUNT         1631 a         1104 c         1237 g         1798 t
ORIGIN      
        1 tagagagttt agtgatcatc ataataatgc caatctaaaa cagggtattg gctaggatgt
       61 atactcaaaa taaagcaact tttaggtaaa aattctcgtt gactttaata ggctgacccc
      121 aaatacaaat aaatattatt atccctggtt attgattttt gaatgagatg agtgaaggag
      181 agattatgga gtttagtagc cggtatgtcg cacatgtccc tgatgctcag ggtttagtcg
      241 attattcggc acaagaaaat agaatttgga atattttatt tgagagacaa ctcaaattat
      301 tgccaggaag agcttgtgat gaatttctgt ctggattaca gactttagga cttaactcct
      361 cgactattcc acaacttcca gaagtaagtg agcgattaaa ggccaaaacg ggatggcaag
      421 tagcgccagt tgctgcttta atttcagcca gggaattttt tgaattatta gcagaaaaat
      481 attttcctgc ggcgactttt attcgaagtg aagaagaatt ggattatgtt caagagcctg
      541 atatttttca tgagcttttt ggtcattgtc ctatgttaac cgatagagtc tatgctgaat
      601 ttgtccatga ttacgcatgt aaggtattaa cttttcctga acaggattgg cctttattgc
      661 aaagaatgtt ttggtttact gtagagtttg gattgattaa aacgcctaaa gggcttagag
      721 catacggcgg gggaatttta tcttctatta gtgaaacggt atattgtgtg gaaagtgata
      781 ttcctgtgcg aattttattt gatccagtgg tggcttttcg aatgccttat cggattgacc
      841 agctacaacc ggtttatttc gttattgaca gctatcaaaa tttatacgac ttcgtgcttt
      901 ctgacatggg taaattcatg gatcgtgcgc gagagttagg tgaatttcca ccgtattttg
      961 atgtggatcc ggaaaatcca aatattcata taagggcttg ttagtttgtt attttgtaag
     1021 acagctggac ttgtggatta gagtgactat gtattgtaat gattatcgag tcttgttaac
     1081 atcgaccaac acagtttgtt cattgactga ttggtcaagg agcattattt gattaaagta
     1141 ttaattgttg atgaccatgc attggttaga atgggcattc gacgattgct tgaagatatg
     1201 tcggatgttg atgtggttgc agatgctgaa agtggtgagc aggctttagc ttatgtgaaa
     1261 acccactccc ctgatgtcgt attattagac atgaaaatgc ctgggataga tgggtgggaa
     1321 gtgacacgtc gtttaaaaaa aacaaaccca aatattaaag tcatagccgt tacagctatt
     1381 tgctcagatc ctctccctac tcgagtattg caattaggtg ctatgggata ccttactaaa
     1441 gaatcagggg ctgaagaaat ggctgctgca attcgtaaag tggctaaagg tgagaaatat
     1501 cttagtgccg agattgcaca aaaaatggca attaatagtc ttcaggagcc tcaggattcc
     1561 ccctttgatt tattgtctga aagagaaatg caagtcatgt tgatgattac gagcggcatg
     1621 aatgtacaag acattgctga tagattattt ttaagtagta aaaccatcaa tggttatcga
     1681 tataggatgt ttgaaaaatt ggggattaaa aatgatgtgg agcttactta tttggctatg
     1741 aagcaccgca ttattgaaca ccccaatgat ttgtcacaag atgattagat gttaatttca
     1801 tgaatgactt acagctttcc gctgaactcg ctttattttt aacgaaactc ccaagtgaac
     1861 cgggtatata ccgcatgcta gacgaggaag gcaccgtact atatgtgggg aaagcagcca
     1921 atcttaaaaa aagagtcaac agctatttta gtaaacaaaa tacaggagtt aagacacgag
     1981 cactggtaag ccaaatcaaa tccatagaaa tttctgtgac aagatcagag acagaggcct
     2041 tattattaga gagtaattta attaaagcgt taaggccaaa atataatgtt ttgttgcgtg
     2101 atgacaaatc ctatccttat attcatctat caaatcatcc agattttcca agaattgagt
     2161 tataccgaag taaaaagaag ccgccctccg gtaatttttt tggcccctat cctggggtag
     2221 ctgctgtaag agaaacaata gtcaccattc aaaaaatatt taaaattcgc aattgtagag
     2281 acagttattt taaagcccgt tctcgccctt gtttacaata tcaaataaaa cgttgtaccg
     2341 cgccttgcgt tcattatatt tctcctgaaa actacaaatt gtccgtagag gatgccataa
     2401 gatttttaca ggggaagtgt caaataattc ttgatgcact tgccgaacga atgaaacaag
     2461 cggtgaatca attaaatttc gaagaagctg ctgttttgcg tgatcagata aaaaatttgc
     2521 gcttaattca agaacaacaa ggagttgtcc agttacgtgg tgatgcggat gtgatcgcaa
     2581 tagaagtacg ccctggtttc gcatgtattc agtgtgtgac tattcgtgaa ggacaagtac
     2641 tgaatagtca aagctttttc cctacagtgc cttatgcagt attagatgaa gaattggatg
     2701 ccaactcact ttggcagcag acttttgagg cctttattgg tttttattat ttggatacaa
     2761 cagaaagaat tccggatttg ataattacta atcaatcaat aacagagagt cgctcattag
     2821 agtatatttt gtcccagcga cgaggcaaat cctgtaaaat tcaagtcaat ccaagaggag
     2881 tcaaatcgag atggatggat tttgcagtga acaatttacg aatttcagta gcagaatatg
     2941 tcagcaaaca ttctacgata agatcaagat atcaggcatt aaagcaattg cttgcgcttg
     3001 ataaaaatat agagcgaatg gagtgttttg atattagcca tactcaaggt gaagcaactg
     3061 ttgcatcttg tgttgtattt gatacagaag gccctcgccc cagtgaatac aggcgattta
     3121 acattgaggg tattactcca ggggatgatt acgcagcaat ggaacaggca gttacccgcc
     3181 gatttaaaag actgattgat gctcaattat tgccagatgt tttgattatt gatggaggta
     3241 aaggccaggt ttctattgta aaacgagttt taacctctct gggtgtggaa gatattactt
     3301 tattaggtgt ttccaagggg ccttcacgaa aggcaggatg ggagaaattg atactggtta
     3361 atgaaaacag agaatttgtt ttgccggagg actcgaaagc gcttcatctt ttacaacata
     3421 ttcgtgatga agcgcatcgt tttgctatta ctgcccatcg gaagaaaaga caaaagacac
     3481 gtgttgaatc cactcttgaa agtatagagg gtgttggtgc gaaacgacgt caagcgttgc
     3541 tgcagcgttt tggcgggttg cgagagttgg ctaaagcgcc actggaagaa atttgtaaag
     3601 tccagggaat cagcgagcag ttggctaagc gaattcatga acattttcat ccttgagcaa
     3661 aattgaagtt ttgccatact taaaaggctc ttacagcacg tacagcagct gttttatttt
     3721 tggcaatgtc tttgatttcc cctgttttag aattaaatac ccagctgttt gccgatgatt
     3781 tttcaatgga actccagtaa ttacctgcca aaaaatttcc cagctcatca cgcatcatat
     3841 aaattaaatt caattcaaaa cggctaggaa gcctccagtc agaatataat ttaccttcat
     3901 tgtcaaagtg ggcaggattg gttatggagt cttgagcatc ccaccatgtg cttgaattaa
     3961 cttgatctga atttgctact acaagcccat gggtacctag cgcattaaca taaattactg
     4021 taccgccaag agctttatcg cctaattgcc gcctaatacc ggaatttgcc gggtcaaccc
     4081 acatggtatt gaaatcgagg tcatttgatt tggcaaggat ttgacctgtt tcaccacctg
     4141 caggcacacc ttggccgtca tctcctgctt cgcctttagg tcctggcata ccttgtggac
     4201 ctactgctcc cgtttctcct ttgtctcctt tagggcctgg taatccctga ggtcctgctt
     4261 ccccttggtc tcctttaggg cctggcatac cttgtggacc tactgctccc gtttctcctt
     4321 tgtctccttt agggcctggt aatccctgag gtcctgcttc ccctcggtct cctttagggc
     4381 ctggtaatcc ctgcggtcct gcttcccctc ggtctccttt agggccgggt aatccctgcg
     4441 gccctgcctc cccttggtct cctttcgggc ctggtaatcc ctgaggtcct gcctctcctc
     4501 ggtctccttt agggcctggt aatccctgcg gccctgcctc cccttggtct cctttcgggc
     4561 ctggtaatcc ctgaggtcct gcttcccctt ggtctccttt cggtccgggt aatccctgag
     4621 gccctgcctc tcctcggtct cctttagggc ctggtaatcc ctgaggtccg gaggggatac
     4681 tgttgatttt gttggttaat tcatttttta attcagatac cttaccgtcc acataggctt
     4741 gcgaggccgg attgcttttg gcaaatgcta tctgtgatat gaataagatt aataaaatgc
     4801 tattaaggac tttatttcga tgtatcattc tttatttccc actatatgct cacttgtctc
     4861 ttttatttta ctgctgcgtt acaagcgttt ctgcggtgcg tatttactgc ttgtaaatct
     4921 gactcctcaa aacgtttgta ccttgcccta aaacaaaata agcaggttat gcaagagatc
     4981 tattagaaaa attagttaaa tcattaaaac aatacctaac aaaatggcgt gttgaacagg
     5041 aatcttttgt acatgcccaa tgccactttc cagatcaata gtgaaatttg gtttgtctcc
     5101 aaataggcct tgataggata gggtaatact ggcatttggt gtaattgtat aaccacatcc
     5161 agccattatt tcgcctgcaa gttgagagat gtcattgatc gtgtctctat ccatttgcca
     5221 ttgcctgtag gcagttcctg ctttcagttg tagaaacaaa ggggcggtaa attcagaggt
     5281 aatacggatt gtagctaatg catcaaccat aggtttcaag gtggtttgta ccaaaagtcc
     5341 tccgagggca tcgacctctt cttccgtcat ttgcagtcgc atttgattcc cactttgtaa
     5401 acccaattca agtccaaata aaaaggggcc tttcatcaac aaatctcttc ctattgcaaa
     5461 ccgacctgtt gcagtaatat ggtcgccttc gtgtacgtat tgataattga cataccccaa
     5521 gcttgcggag actgtccatg gataattgat tgttttagcg aggcttgaat tactaaagaa
     5581 gagaataagc acaagtattt ttagaataag tttgttattt aagatatttg gttggttgga
     5641 aaaacaaaaa ggcataagct aacttccatt gcaatgtatg attcttttat caagtattta
     5701 agtcaattaa ttatcaggac ttacgaaaaa cccccatctc tttgttaaaa aatgttttta
     5761 attcggtcat
//
LOCUS       DAXLDT010000182         5731 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXLDT010000182 DAXLDT010000000
VERSION     DAXLDT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.182"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            556..1479
                     /locus_tag="ACF2SP_002707"
     CDS             556..1479
                     /locus_tag="ACF2SP_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFD2360476.1"
                     /translation="MAEKSYTKPALTVSQQLEFLFSQGLQIEDQKLAIRALETVSYYR
                     LSSYLLPFKQSHNAKNPRQFKENATFEQVWQLYQFDRELRLLVADAIEKIEVAFRAAL
                     TNVTSIRFNPFWYVERNYFKAKAGANRERDFFDDYLKTIKTISTNKQELFIQHYHKNY
                     DKPYFPPIWMMVEALSFGVCSKMFNNIQSKDVRNEIASFLGQHTTVIESWIKSLTYTR
                     NLCAHHSRLWNRWLVIPPLIPKNAPIKTHIDGNYRFQLIAFIIDQLLETIAPESNWKI
                     KLFELFERNEQFSGIEMGFINNWRDDPIWHC"
     gene            complement(1533..1916)
                     /locus_tag="ACF2SP_002708"
     CDS             complement(1533..1916)
                     /locus_tag="ACF2SP_002708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360477.1"
                     /translation="MLNFIILLEKQLKKQALLLISFAFNKAILTKQPDAKIVIPPPSV
                     AVISWKANTQRDDHIRLLQDEGDMVWQKKNNYGLRSHIELAILRYKKVMGTAMKAREL
                     PQQKTECGIATRALNESLHWVCQSL"
     gene            complement(2010..2315)
                     /locus_tag="ACF2SP_002709"
     CDS             complement(2010..2315)
                     /locus_tag="ACF2SP_002709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360478.1"
                     /translation="MSFQARLDIIIIKKSGPEEGVYIRAGTTNRKADTEMIKELNRIV
                     SNQTFDEQPLTALNSEAIDFRAALEQFEKIRKIDKKDLETVVILMDYQGKKVPTVQG"
     gene            complement(2644..3198)
                     /locus_tag="ACF2SP_002710"
     CDS             complement(2644..3198)
                     /locus_tag="ACF2SP_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFD2360479.1"
                     /translation="MQRIKKISIANAQGKAKELLEGTKKAMGTELNLFSTFANSPAAL
                     EAYIGIMTSLSKGVLNPKLREQIALVSAGYNGCNYCASAHTYLGEKAGINKDELKENL
                     SGKSSDKKTQVALNFATQLIERRGGVSEFDIKTVREAGFSDEEMVEILAHVAMNTFTN
                     YFNEAFKTDIDFPVVDTHKGRTAT"
     gene            3370..3891
                     /gene="msrB"
                     /locus_tag="ACF2SP_002711"
     CDS             3370..3891
                     /gene="msrB"
                     /locus_tag="ACF2SP_002711"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFD2360480.1"
                     /translation="MKSIIKGLLFFFMSVASLTAYAEQAYKRPSDAVIKQKLSTIQYN
                     VTQQQGTESPFNNAYWNNEEQGIYVDVVTGEPLFISLDKYDSKTGWPSFTKPLEPGNI
                     IYKDDSSMFTSRTEVISKHGKSHLGHVFGDGPPPTNKRFCMNSAALEFIPVKDLQKRG
                     YGKYLPLFKNAGK"
     gene            4049..4276
                     /locus_tag="ACF2SP_002712"
     CDS             4049..4276
                     /locus_tag="ACF2SP_002712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360481.1"
                     /translation="MKFYLHPSCHLLSMHWNSFDLIKASRDGYKHPPRPKKMAHSQSL
                     VIWRRQLQIRYYLMDKLEQNMLSQSKTRLLS"
     gene            4850..5308
                     /locus_tag="ACF2SP_002713"
     CDS             4850..5308
                     /locus_tag="ACF2SP_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HFD2360482.1"
                     /translation="MKKIKSLYIPQLIFIFMMPVLAWASDISYSLVGIQGYDPVSYFQ
                     QGGPVRGSGNYTGYYEGVSYIFSNQENKKTFLANPEKYLPAYGGYCAYGVSVGSKIVS
                     DPLAWRIVDGTLYLNLNSKTQDVWAKNIPGNIKKANINWPKIKGIDPKDL"
BASE COUNT         1761 a         1032 c         1092 g         1846 t
ORIGIN      
        1 ccacctgtta tggatcagtc actgccagca gctccaggaa cttatgtgct tcagaaatag
       61 aactcgtgtt agggtgttgt gttgttaaat ggtgagtgcc cccgtaattg ggaaagagcc
      121 ggaatagacc gattaaaaat ggcgcgctcg gagggactcg aacccccgac accttggttc
      181 gaagcctgat actcaataat agaaaacatt ataaatcaac aacttacgat gcatcaaatc
      241 atcgaattac tgcctagaac tgccacaaat acactacaaa cggtcactgt tacggcacga
      301 tttcgtcact gttgtgtcag gcagcgctag gcagtcccaa tcaatatttc cctgtgcgac
      361 atacagccac attagagtcc tattgttgat tattagtatt ttgattagac tgactctgaa
      421 tggtataatc tcttattacc tgagttaata aaaatacaaa acgcgtattt gaatttgaga
      481 gtggttgcgc tataatataa ttaaccatgc ccgtcacgcc tctcaatgat gcgcaccacg
      541 acgggttatt tttttatggc tgaaaagagt tatacgaaac ctgcacttac tgttagtcaa
      601 cagctggagt ttttattttc tcagggtttg cagattgagg atcaaaaact agctattcgt
      661 gcattggaaa cagtaagtta ttatcgctta tcatcctatc ttttgccttt taagcaatcc
      721 cacaatgcaa agaaccctcg ccagtttaaa gaaaacgcta catttgaaca agtttggcag
      781 ctttatcaat ttgacagaga gctaaggtta cttgttgctg atgcaattga aaaaatagag
      841 gtggcatttc gagcggcatt aaccaatgtg acaagtatcc gatttaatcc tttttggtat
      901 gtagaacgaa attattttaa ggctaaagct ggtgcaaata gagaaagaga tttttttgat
      961 gattatctaa aaacaattaa aacgattagt accaataagc aggaactatt tattcaacat
     1021 tatcataaga attatgataa accatatttt ccaccaattt ggatgatggt tgaggcactc
     1081 tcctttggtg tctgttctaa gatgtttaat aatatccagt cgaaagatgt gcgtaatgag
     1141 attgcttctt ttttaggtca gcatacaact gtaattgaat cttggataaa aagtttaacc
     1201 tatacaagga acctctgtgc tcatcattca agattatgga atagatggct tgttattcct
     1261 ccgctaatac ctaagaatgc gcctattaaa acccatattg atggtaacta tcgttttcaa
     1321 ttaatcgctt ttatcataga tcaattatta gaaacaattg cacctgaatc taattggaaa
     1381 ataaagctct ttgagctatt tgaaagaaat gagcagtttt caggaattga aatgggattt
     1441 ataaataatt ggcgggatga tccaatatgg cattgttgaa taaatcaaaa ttaggcaaca
     1501 tttcacctaa ccgaattggt tttacttcac tttcacagag actggcatac ccagtgaagt
     1561 gattcgttta aagcacgcgt agcaatccca cattctgttt tttgctgagg taattccctt
     1621 gctttcatgg ctgtccccat gacttttttg taacgcaaaa tagctaactc tatatggctg
     1681 cgcaaaccat aattgttttt cttttgccat accatatccc cctcgtcctg caaaaggcga
     1741 atatgatcgt cgcgctgagt gttcgctttc cagctaatca ctgcaactga tggaggagga
     1801 atgacgatct ttgcatcagg ctgtttcgtc agtatagcct tattaaaagc aaagcttatt
     1861 aaaagcaaag cttgcttctt taattgcttt tccaggagaa ttatgaaatt cagcattatc
     1921 tacaataaag ctctcgtctg ttccttgaaa tcgacccgct tgaatccatg catctggaaa
     1981 tattttggct ctgtccttac caaataatat taaccctgaa ctgtaggtac ttttttgcct
     2041 tgataatcca ttaagataac gaccgtttct agatctttct tatcgatctt cctaattttt
     2101 tcgaattgct ctaaagcagc tctgaagtct atcgcttcgg agttcaatgc agttaagggt
     2161 tgctcatcaa aagtttgatt gctgactatg cgatttaact ctttaatcat ttctgtgtca
     2221 gcttttctat ttgtggttcc agcacgaata tatactcctt cttctggtcc tgattttttt
     2281 ataataatga tgtctagtct agcttggaaa gacacgaaca attaaaagat aaatatcccg
     2341 ccatagaata gtatctattt cgggaaaaat gtgtggggca attgatcgct aataagaatg
     2401 gcaacttttt ctctgatgtt ttttgttcta aaataaaaac gtagatacca aaaagcttgt
     2461 ccattgagtc aattggtatt tcgagatctc tggatcttcc agttcaaccg ctgtaaatcg
     2521 acgttctata aagtcaagaa taaaaacttg aggattgaat cttagggtgg tttgtatttg
     2581 gggcaaaata taatttggat tttctattaa ggaggtggtt tttaagccac cccttagaat
     2641 aatttatgtt gcagtacgtc ctttatgggt atcaacgaca gggaagtcga tgtccgtctt
     2701 gaatgcttcg ttgaagtagt tagtgaaagt gttcatcgct acatgcgcta aaatttcaac
     2761 catttcttca tcactaaatc cagcttcacg gacagtcttg atgtcgaact cactcacccc
     2821 accacgacgt tcaatcagtt gagtagcaaa attgagagcc acttgtgttt tcttatcaga
     2881 cgattttcct gagaggtttt ctttaagttc atctttatta atccctgctt tttctccaag
     2941 ataggtatga gcggatgcgc agtagttaca gccattataa cctgctgaga ccaatgcgat
     3001 ttgttcgcgt agtttagggt taagaacacc tttgctaaga gaggtcataa tcccaatgta
     3061 ggcttccagt gccgctggtg agttggcaaa tgtgctgaat agatttaatt cagtgcccat
     3121 agcttttttt gtgccttcga gtagctcttt agctttgcct tgtgcgttag cgatagagat
     3181 ttttttaata cgttgcacga tacttctcct tatttttgga tagattggaa tacgacagta
     3241 ttctcttgcc ctttatattt gtagcagacc atataactta cgtcaaggaa attgacagtt
     3301 gttcggaatt ctgttgttcg gaattctgac tatacttaag aatagagcat taatcagtga
     3361 gaaaccacca tgaagagtat tatcaaaggt ttgttatttt ttttcatgag tgttgcctct
     3421 ttgacagcct atgcagaaca agcctataaa cgaccttcag atgctgtcat caaacaaaaa
     3481 ttatcaacaa ttcaatataa tgttactcaa caacagggta cagaatcccc gtttaataat
     3541 gcttattgga ataatgaaga gcaaggtatt tatgttgatg ttgttaccgg tgaaccatta
     3601 tttatttcgc tcgataaata tgactctaag actggctggc cgagttttac caaaccgcta
     3661 gaacctggaa acataatata taaagatgat agcagcatgt ttactagtcg gactgaagtg
     3721 atttccaaac atggaaaatc acatttgggg catgtttttg gtgacggccc tccgcccacc
     3781 aataaacgtt tttgcatgaa ttctgcggcg ctggaattta ttccagtcaa ggatctgcag
     3841 aaaagaggtt atggtaagta tttgccacta ttcaaaaatg caggaaaata acgtgaagta
     3901 atatgccatg tttatggctc acaacaagat ttgcctttga taaacagagc ttaggcagtt
     3961 gagtggtttt gcctggtttt tgcaatttat caattaatca gctaccttta agctggataa
     4021 tattatcaac agggagctta aacctgacat gaagttttat cttcatccat cttgtcattt
     4081 attatcaatg cattggaaca gttttgacct cattaaagca tcaagagatg gttacaagca
     4141 tcctcctcgt cctaaaaaaa tggctcattc acaatcttta gtcatttggc gccgccaact
     4201 tcaaataaga tattatctaa tggataagtt agagcaaaat atgttaagcc aatccaaaac
     4261 aaggcttctt tcatagaaat ttgtgaacaa ttaagctatg aaatgaagga tgagaaagcg
     4321 gcaagctatt tggtaaaaaa attgtatgtt tggcttgagg aacaattgtt tgtccatcaa
     4381 ttgaatcctt ttgtctaaaa cactattatg cctcgtttct agtcattcat taaaatcatt
     4441 ggttttttct atctgtattt ctttttctta atggaggtaa taagtaaatt atttgctcta
     4501 aaagtaaaaa aggaccatac tcaatcggac agcacagaga atttatttcg tgctcctaca
     4561 aggaaggata catggacaac tcctcaaaac caccaggcaa gcgctccata aaagctgaaa
     4621 tgatttaatt caactattga tgcttttcta ataatggtcg ccaggctgcc taaatgtgtg
     4681 taattaaaag tagcattgga tggtttatta taaacaagag ccgcaatttt tttggcaaca
     4741 aaagcgccgc tttgttatgc ggcaggtgca agtccaggga aagattttcc gtaccacttc
     4801 aatgtaaaaa gatttaaata tggttaacaa ttttataagg atagcagcga tgaaaaaaat
     4861 taaatcattg tatataccgc aattaatatt tatttttatg atgccagttc ttgcctgggc
     4921 ttcagatatt tcctacagtt tggtgggtat tcaggggtat gatcctgtgt cttattttca
     4981 acaaggtggg cctgtacgtg gtagcggaaa ctacacggga tattatgaag gtgtgagtta
     5041 tattttttct aatcaagaga acaaaaaaac atttcttgca aatcctgaaa aatatcttcc
     5101 cgcttatgga ggatattgcg cgtatggcgt atccgttggg agcaaaatag tgagtgatcc
     5161 tttagcttgg agaattgtgg atggaacact ctatctaaat ttaaacagca aaacacaaga
     5221 tgtttgggca aaaaatatac ctggcaacat aaagaaggca aatattaatt ggccaaaaat
     5281 taaaggcatc gaccctaaag acttataata agttgcactg ttttaatata attctccttc
     5341 ccacttagtt tgcatccgga tttgttttgc ggttcgaatt aagaagtttg gaaggggaaa
     5401 tgatgtaatg agtatgtatt cattaagtaa agattcggga aattgtggtg attaatacag
     5461 actctatagc ctttaaacag actaccgtca gcggtaagcc agtaatcgtg gctgggaaag
     5521 ccttgacatc ttttaatatt cacctaaact tttcattaaa acgttgagct taatatatcg
     5581 ataatatcat gttcattagc tgaagaagga atatgaaaga aaaaaaaagg agtaaaacaa
     5641 gtaaatacct aagtaattta gttttaattc ttgctctctc catgatggtt agctcagctg
     5701 tgatttcata tttctttatc agacaaaatg a
//
LOCUS       DAXLDT010000183         5702 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXLDT010000183 DAXLDT010000000
VERSION     DAXLDT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.183"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            93..344
                     /locus_tag="ACF2SP_002714"
     CDS             93..344
                     /locus_tag="ACF2SP_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017020027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFD2360483.1"
                     /translation="MQTLYATQVASISELKKNPTKLINDAHGMPVVILNHNVAAAYLI
                     PAETFEKLMDAVDESELKSIVEQRLSEPFTPVKVALDDL"
     gene            334..621
                     /locus_tag="ACF2SP_002715"
     CDS             334..621
                     /locus_tag="ACF2SP_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFD2360484.1"
                     /translation="MIYELHFHPLALKEWKKLSKELQEQFKKLLKRRLENPHVISAKL
                     SGHLKDCYKIKLHQAGYRLVYQVNDNKMILLVVAVGKRNKNKVYESAEDRT"
     gene            834..1595
                     /locus_tag="ACF2SP_002716"
     CDS             834..1595
                     /locus_tag="ACF2SP_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD2360485.1"
                     /translation="MAYTINQLARLSGVSTRTLRFYDEIALLKPAYYGGNQYRYYKEE
                     QLLMLQQILFFRELGFPLNDIQQIMSSDDFDKIESLKRHKSALESSLERTATLIKTID
                     KTISHLRGKIIMRDAEMYDGFDPIKQQEHEKYMLETGVISQKQIDDSWTRVSHWKKSN
                     WEQFKNAGEKLNLELANALAKGVRTDSDEVQALIQEHYDWVNNFWTPTKETYLGLGKM
                     YLDHPDFRLFYNKYHPGLVDYLVAAMRVFAERKLS"
     gene            1653..2528
                     /locus_tag="ACF2SP_002717"
     CDS             1653..2528
                     /locus_tag="ACF2SP_002717"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2360486.1"
                     /translation="MNTKICFTNSTILLNDGRTLGYAEFGDPKGEVVFYFHGLPGSRL
                     EAGHWENIACLNHYRLISIDRPGMGLSSKHPTRTILSWADDVEALANYLGIPKFSIIG
                     HSGGAPFVAGCGYKIPHRLNKIAIVSGMGPFEIPEATASLGRGQRFINKMIKAIPPIA
                     TVMVNLMFLMLKKPGILKKMTSKMSEVDQRILGDTEAGDLFIQSSLEAFKGGITGVSQ
                     EIQLSLKPWGFDMSHIKCPVVIWQGRLDKQAPLAHANLYAKLIPNASLKVLDHEGHIS
                     LLINHGEKILRSVRE"
     gene            complement(2622..2804)
                     /locus_tag="ACF2SP_002718"
     CDS             complement(2622..2804)
                     /locus_tag="ACF2SP_002718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360487.1"
                     /translation="MLPKNASLGHIALAVNKLAECEQFYNLLGLQTELKTMGYVYLTG
                     NSDNCEIGGKSLNLTL"
     gene            complement(2860..3171)
                     /locus_tag="ACF2SP_002719"
     CDS             complement(2860..3171)
                     /locus_tag="ACF2SP_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFD2360488.1"
                     /translation="MYMLCFCVPETHLEIVKNAIFATGAGSVDHYQHCAWQTLGEGQF
                     MPLPGSHAFIGEINQLERVPEYKVEIICTEEQITVAVAALKKAHPYESPSYQAFRVES
                     I"
     gene            complement(3180..3755)
                     /locus_tag="ACF2SP_002720"
     CDS             complement(3180..3755)
                     /locus_tag="ACF2SP_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HFD2360489.1"
                     /translation="MPFNMLKEWLNKEKERGIEDPSCAVLSTCSSSSEPHSRVVAIRE
                     IETESLLFFTQQKTRKVAELLNNPKSCLNFLFAMQNRQVILEGTAIPISQEENEAFWS
                     TLPRERQLRFSAYAPTSGLVIKDLNQLETRKKELSDQFAGLPIPMSEYYFGFRFIPET
                     WIFYTVGSISFSEVIRYTKIEDSWKSELISP"
     gene            complement(3806..5314)
                     /locus_tag="ACF2SP_002721"
     CDS             complement(3806..5314)
                     /locus_tag="ACF2SP_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFD2360490.1"
                     /translation="MTIEQNELEIKNNLHQSITWGQPIPLSPILAPDCPYPVEALPSI
                     VKKAVVDYQQYGQQPLPLIACSALANMSLSCQSLANVARDNYLISPVSLYFLIASGSG
                     TRKTAADNVFSRAARHWETLIRKRREPEVLSALTQHQVWQMEKDSLFSQIKRATYTGE
                     DSDYAKEMLDELIHQEPEIPLQPTLYFEDATQEALAIHLAQGWPSASLWSDEAGIILG
                     SHSMQGNPTRFVALLNRLWDGKTFTAHRKTTQGFTIENRRLTLNLMMQPLILQQLTKQ
                     ASGISRQSGFLARCLMAYPANSMGNRFYQEPPASLHSLTHYEQRVTACLNQSEQLSHQ
                     GCIQLPLLKMSMAAKQQWILFFNAVEAGLKPQGQWVDVVDFASKAAENTARLAALFHL
                     FEGRHGDIGVEHTENAIEIISWHLQEARRIMPTETVSLQFSDALKLMDWFIEKNISST
                     TLRTIQQLSPLRDKIKRDQAVELLVEHNQIRIVKSGSKTMVEVNPRACKVPT"
     gene            5463..>5702
                     /locus_tag="ACF2SP_002722"
     CDS             5463..>5702
                     /locus_tag="ACF2SP_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360491.1"
                     /translation="MIVKCVRAGVHELKQINELMYRSKSYWGYDKAFMDKFMQLFQMT
                     TDYLEKNTVQLFCERNTEKPEKVMGFYSFSIRNKES"
BASE COUNT         1770 a         1011 c         1154 g         1767 t
ORIGIN      
        1 aatccccttt ataaatttcg tgatgattga ccttttatta taaaaagcac tataattaag
       61 tcataataaa ggtatttatt taggtcatta aaatgcaaac tttatatgca acacaagtcg
      121 ctagcattag cgaattaaag aaaaatccca caaaacttat taatgatgcg catgggatgc
      181 ccgtggtgat tcttaaccat aatgtagctg ctgcttattt aattccagca gaaacttttg
      241 aaaaactgat ggatgctgtt gacgaaagcg agctaaaaag cattgtagaa caacgccttt
      301 cagagccctt tacgccagtt aaggttgcgc ttgatgatct atgagttaca tttccatccg
      361 cttgcgttaa aagaatggaa gaaactatcg aaggagttgc aggaacagtt taaaaaacta
      421 ttaaaacggc gactggaaaa tccgcatgtt atttctgcca aacttagtgg ccatcttaaa
      481 gattgctata agataaaatt gcatcaggct ggctatagat tggtctacca agttaacgat
      541 aataaaatga ttctccttgt tgttgctgta ggcaagcgaa acaaaaataa agtctatgag
      601 tcagcagaag ataggactta agctgatgat gaatagggta tttgatggtg tgcacctgaa
      661 gtgattctta aaaataacaa ctacagaaac ttgctaattt aacttagttt atgcaatcaa
      721 tttgatgact ggattaatgg tcgttttaac aggtttaaaa aaattattat atctattgac
      781 cttgccgtta cgtcaaccct tatcgtgtat gttgtcaatt agaggagagc aaaatggcat
      841 acacgattaa tcaattggcg aggctttcag gggtcagtac ccgcactctg cgattctatg
      901 atgaaattgc tttattaaaa cctgcctact atggtggcaa tcaatatcgt tattacaaag
      961 aagagcagct gcttatgtta cagcagattt tattttttcg tgagcttggt tttcctctaa
     1021 atgatatcca acagattatg agtagtgatg attttgataa aatagaatca ttaaaaagac
     1081 ataaatctgc tttggaatct agtcttgaac gaacagcaac gctcatcaag acgattgata
     1141 aaaccatttc acatttaaga ggaaaaataa ttatgcgtga tgcggaaatg tatgatggct
     1201 tcgatcccat caaacaacaa gagcacgaaa aatatatgct cgaaacagga gtgatatcac
     1261 aaaagcaaat tgatgatagt tggacgcgtg tttctcactg gaaaaaatca aattgggaac
     1321 agtttaaaaa tgcgggtgaa aagcttaatc ttgagctggc caatgcatta gcaaaaggag
     1381 ttaggactga ttcggatgag gttcaggcat taatccaaga acattacgat tgggttaata
     1441 atttttggac accaacaaag gagacatatt taggacttgg aaaaatgtac ctggaccatc
     1501 cagattttcg attattttat aataaatatc accctggttt ggttgattat ttggtagctg
     1561 ctatgagagt ttttgctgaa cgtaagctat cgtgatcatc ttgctctgct gatagaagca
     1621 tcagtagagc actatctttc tgggaaatta ttatgaatac caaaatttgc tttactaatt
     1681 ctactattct gttaaacgat ggcagaacac ttggatatgc tgagtttgga gatccaaaag
     1741 gagaggtagt tttttatttt catggacttc caggttctag gcttgaggct gggcattggg
     1801 aaaatatagc atgtttaaat cattatcgat taattagtat tgatagacca ggaatgggtt
     1861 tatcttctaa acatccaacg cgcactattc tttcatgggc tgatgatgtt gaggctcttg
     1921 ccaattactt aggtatccct aaattttcta ttataggtca ctctggtgga gctccttttg
     1981 ttgcaggatg tggatataaa atacctcatc ggttaaataa aatagcgatt gtctcaggta
     2041 tggggccttt tgaaatacct gaggctacag caagtctagg acgaggacag cggtttataa
     2101 ataaaatgat taaagcaata cccccaattg ctaccgtcat ggtaaatctc atgtttttga
     2161 tgctgaaaaa acccgggata ttaaaaaaaa tgacgagtaa aatgtcagag gtagatcagc
     2221 gtattttagg tgacacagaa gctggtgatt tatttatcca atcatcgtta gaggctttta
     2281 aaggtggtat tacaggcgta tctcaagaaa ttcaattaag cctaaagcct tggggatttg
     2341 atatgagcca tataaagtgc cctgttgtca tttggcaagg tagattagat aaacaagctc
     2401 cgttagctca tgccaatctt tatgcaaaat taatccctaa cgcctcacta aaagttttgg
     2461 atcatgaagg acatatctcg ttgctcatta accatggtga gaaaattttg cgcagtgttc
     2521 gtgaataaaa ttttgcatcg tcgctactat tttaggttac aaccgtattg gaagtaacga
     2581 tcgtgataaa agtggcatac aagagaacat ttttatttat attagagcgt tagattcaac
     2641 gattttcccc caatttcaca attgtcgcta ttgcctgtaa gataaacgta gcccatcgtt
     2701 ttaagttctg tttgcaatcc caataaatta taaaactgct cacattcagc aagtttatta
     2761 actgctaggg ctatatgtcc caaactggca tttttgggta acatttttta ggtccttatt
     2821 tgaatgtaag ttggttcata ttggaagcat tgtttattat tagatactct caacccgaaa
     2881 cgcttgataa gaaggtgatt catagggatg agccttcttt aaggccgcaa cagccactgt
     2941 gatttgctct tcagtacaga taatttctac tttatattca ggtactcttt caagctgatt
     3001 gatttcccca ataaaagcat ggcttccagg taatggcata aattgcccct cacccaatgt
     3061 ttgccaagca caatgctgat aatgatcaac actccctgcg cctgtagcaa aaatagcatt
     3121 tttaacaatt tctaaatggg tttctggaac acaaaaacaa agcatgtaca tgatatcacc
     3181 tatggggaaa ttaattctga tttccaagaa tcttctatct tcgtatagcg aattacttca
     3241 gaaaaagaaa tagagccgac cgtgtagaaa atccatgttt ctgggataaa gcgaaaaccg
     3301 aaataatact cgctcatagg gataggaagt cctgcaaatt gatcactcaa ttcttttttc
     3361 ctggtttcca attggtttaa atctttaatg acaagtcctg aagtaggagc ataagcagag
     3421 aatctcaatt ggcgttcacg aggaagagta ctccaaaaag cttcattttc ttcttgagaa
     3481 atggggattg ctgtaccttc taaaataact tgtcggttct gcatcgcaaa taaaaaattt
     3541 aaacaggatt tagggttatt taataattca gcaaccttgc gtgttttttg ctgcgtaaaa
     3601 aataaaagac tctctgtttc aatttcccga atggccacca ctcgactatg aggttcgcta
     3661 gagctgctac aagtacttaa aacagcacag gaaggatcct caatccccct ttctttttcc
     3721 ttgttcaacc attcttttag catgttaaag ggcacgagtt tctcctgtaa attggtgtga
     3781 taaagttaaa agtattaaac aaaactcatg ttggcacttt gcatgcgcgt ggattcactt
     3841 ccaccatcgt ttttgaacct gattttacaa tgcgtatttg attgtgctcc actaataatt
     3901 caaccgcttg gtcgcgtttt attttatctc gcaatggact taattgttga atagttcgta
     3961 aggttgttga actaatattt ttttcaataa accagtccat aagttttagg gcatcggaaa
     4021 actgcaaaga aacggtttca gtaggcataa ttcgtcgtgc ttcttgcaaa tgccaactaa
     4081 ttatttcaat ggcattttcg gtatgctcta caccaatatc cccatggcgt ccttcaaaaa
     4141 gatgaaagag tgctgcaagc cgcgctgtat tttcagcggc ctttgacgcg aaatcaacga
     4201 catcgaccca ctggccttga ggttttaacc cagcttccac tgcattaaaa aataaaatcc
     4261 attgttgttt tgcggccata ctcattttca acaatggaag ttgaatacaa ccttgatggc
     4321 ttaattgttc tgactggttt aagcaggcag tgacgcgttg ctcataatgg gttagagaat
     4381 gtaaagaggc aggcggctct tggtaaaatc gatttcccat actattggca ggataagcca
     4441 taaggcaacg agctaaaaaa ccgctttgtc ggctaatacc ggatgcttgc ttggtaagtt
     4501 gttgtaagat aagcggttgc atcatcaagt taagtgttaa acgccgattt tcaatggtaa
     4561 atccttgcgt tgtcttacga tgtgctgtga acgtcttacc gtcccacaaa cgattaagca
     4621 atgctacaaa acgagttgga ttgccttgca tgctatggct acccaagatg atgcctgcct
     4681 catcagacca taaggatgca gaaggccaac cttgggctaa atgaatagcc agtgcttctt
     4741 gggtagcatc ttcaaaataa agagtgggtt gcaaaggaat ttccggttct tgatgaatta
     4801 actcatcaag catttctttg gcataatccg agtcttctcc cgtataggtt gcacgtttga
     4861 tttgggagaa taaactgtct ttttccattt gccatacttg gtgttgcgtt agcgctgaca
     4921 acacttcagg ttcgcgcctt ttacgaataa gtgtttccca gtgacgcgct gccctcgaaa
     4981 aaacattatc tgcagcagtt tttcgggtgc cagagccgct ggcaattaaa aaataaagcg
     5041 atacaggact aattaaatag ttgtctcgcg ctacattggc aagtgattga catgataaag
     5101 acatgttagc caaggcgctg catgcaatca aaggcagagg ttgctgtcca tattgttgat
     5161 agtctactac ggctttttta acaattgagg gaagtgcctc aactgggtaa ggacagtcag
     5221 gggctaagat tggggaaagt ggaataggtt gtccccatgt aatgctttga tgtagattat
     5281 ttttaatctc taattcattc tgttcaatag tcattgtttt agaggataaa gttttggttt
     5341 tcgattttac cagtagcctc agtatgatgt aaaattagtg gctgaaatat actgatgatg
     5401 ctaaatactg gctctatggc gattcggata tagcgcttct attaaaaatt gttagggtct
     5461 agatgattgt aaaatgtgta cgagctggtg tccatgaatt aaaacagatc aatgagttaa
     5521 tgtatcgctc aaaatcttat tggggatatg ataaagcatt tatggataaa ttcatgcagc
     5581 tttttcaaat gaccacagac tatcttgaaa aaaatacagt gcaacttttt tgtgaaagaa
     5641 atactgaaaa acccgaaaag gtgatgggtt tttatagttt tagcatcaga aataaagagt
     5701 ct
//
LOCUS       DAXLDT010000184         5697 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXLDT010000184 DAXLDT010000000
VERSION     DAXLDT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.184"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(276..2501)
                     /locus_tag="ACF2SP_002723"
     CDS             complement(276..2501)
                     /locus_tag="ACF2SP_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFD2360492.1"
                     /translation="MAESKLEIVNKREREESVILDGNGILRCYNMFPTQFGSIEEMID
                     YLPQLQAMGFNAVWINPVQTAGDVQGLFKRDKTIGVRAYNEVTRSLYAMTDCDLISPY
                     FNPLPPNVDPKLKKQLDMDVMQKFTSEARNNGLVPMFDLVLNHVAADSRHRKEHPEWF
                     QKDVHPDFKDAIAFDYSDPQIRDEIIEQFWKPYIYKYMIDYGFEGVRIDAVGYLNPEL
                     RKQIYAYIYDLAEQFNKPKPVILDELLFSGSQKLTDVVDGLLLPEKGPTHITRGTYYA
                     QRDSYGGLPGWCKEEEGVKAQVVFLDKQKKLHPHAKGGCIAFSGNHDHNSLAMTILEE
                     MAEHRLANHHTLHGAQEKFKHERSYDYDEALESVLRYSFVKDIQNEILSGNESTIKEV
                     ETRMREKIAFCALTSSGGWYALSGDETGDLLAKPVFRRVHALDQTYYAQRTHIIFDKK
                     YPDNKVAMEVLTEMAIENIQKEKAGSIYNNLQHDKQSQGRLLVPYLETLQNQINCGDT
                     VVCKAFQEKLAQKNITIGFTDENYSPDPRTPQNGWNGQHDMRKFMFEINSILSSLPSS
                     NMGFWSEVIPIQSKPGLLAVVRKNGLGLSSETDIAIVNMDPSQKVELTRDDIHQLACN
                     FQKRVIPEYTYYNDGKDKQYNWHTNNPDFNMAYHTVMSCIEAKRIHVDDSIKTNFPVH
                     SANPYGMFSKKQSSKMNIAKIAKMSGEDTTLIEKGTNDTEGIIHDDEQIQPKITVENK
                     K"
     gene            2804..3694
                     /locus_tag="ACF2SP_002724"
     CDS             2804..3694
                     /locus_tag="ACF2SP_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1670 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360493.1"
                     /translation="MGSSYNDWLKSSYSELKKINIVESLIKENNFARAKMLLNNLDLT
                     TLIKYTELSKTITDFCEEAEQADIWRTHLQNFNEEHFSFEEYPPLTVSQLVKGIYFYG
                     QAAECREEEGKPFSDNELEFLKKSAHQHCFYAYNSLSTWAYEKYKMGLNDYSLLTLHY
                     AQKASQYHWTPGYLLFYKTCLNLAILSNAPSLSYQEALEALLIARKLSEHQYSISAIN
                     NAYFGKGLIHGNKTQIESWDKAISETIAKGKIPSTLLNKIYDKASEKAKGILDEFTVE
                     VKDMPEEEKLENEVAPTLYI"
     gene            3798..>5697
                     /locus_tag="ACF2SP_002725"
     CDS             3798..>5697
                     /locus_tag="ACF2SP_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFD2360494.1"
                     /translation="MGYHFLAEGNGIIACYDMYPTQFHSIKDMINYLPVLKQMGFNAL
                     WINPMQMPGDISGFFKTDKNNGVKTGNEVTRSLYAMSHPLLFNPQFSLDSPEDPMETT
                     QRLNSEALQLFTQNARNLGIVPMFDLVLNHVAIDSPLCQDKPHWFKGVHEDFKDVRGF
                     NYDDESIRKEIITEFWQPYIKRYMIEYGFDGVRVDAVGYVHPAVRSEVYAYIHSLAAE
                     YGKPNPVILDEALFSKRPLADEVNYLKLPGIGPTHITTEAYNVEFDLASADLPYEITL
                     EEQLKASVVFQQKDGTWRENTKGGCINFCGNHDYRSLAMTILFQMAKKRLKSDAFYND
                     LISSYQDLYFKFNAEQEKPHELKEPLKTTLLYSYVDQIKKELEDNKDETAQEFKTLVL
                     EKLALSALTASGGWFLLSGDETCDITAKTVFQRKGAVDKSYYPQREHRIFSEKPVIAH
                     KILEKMAEENFFIENSENKWMQELYRSLSSFPEMQKRLLVSHIDNIKHQINAGIDHVQ
                     EKFSRLLASESLNIGFTSQDFLENPRTHENGWLGLQDNFLFIKQLNRLLKTLPAPLPG
                     FSSELVRLPEKPHLIIIVRKNGNELNAPIDVVVVNLEQEKRQTLTKEDFQLIAKNYCN
                     QYFSKNRPS"
BASE COUNT         1824 a         1049 c          987 g         1837 t
ORIGIN      
        1 ttactatctc aggacttacg aaaaactcca aggtcaaggc aaaaaatgtt tttaatgagg
       61 gagtttagat aaactaattc aactaaatga ccgaattaaa aacatttttt aatatagaga
      121 ttggagtttt tcgtaagtcc tgtatcttac cgtttattat ggccgagcac cctacttgtt
      181 atacggtctt tgtaattagt tatattgcag agtatccgat ttatcaaaat aatacccggt
      241 cgccaagttg aaacaacttt tattatagta cttctttatt ttttgttttc tacagttatt
      301 ttgggttgaa tttgctcatc atcgtgtatg ataccttcag tatcattagt acctttttca
      361 attaaagtgg tatcttcccc ggacattttt gctatcttgg ctatattcat tttcgatgat
      421 tgctttttgc taaacatgcc atagggattt gctgaatgaa cagggaaatt agttttgatc
      481 gaatcatcaa catgtatcct tttggcttcg atacaggaca tcactgtatg ataagccata
      541 ttaaaatcag gattatttgt atgccaatta tactgtttgt ctttaccatc attgtaataa
      601 gtatattcag gaattactcg tttctggaaa ttacaggcta attgatgaat atcgtctctt
      661 gttagctcga ctttttgact tggatccata ttaacgattg caatatcagt ttcagaactt
      721 aagcccagcc cattcttacg tactactgca agcaaccctg gttttgactg gattgggata
      781 acttcactcc aaaatcccat attggaactt ggcaaggaac ttaatatact attgatttca
      841 aacataaatt tcctcatatc atgttggccg ttccaaccat tttgaggtgt tctgggatcc
      901 ggactataat tttcgtctgt aaaaccaatc gttatatttt tctgggcaag tttttcttgg
      961 aatgctttgc aaacaactgt atcgccacaa ttaatttgat tttgcaaagt ctccagataa
     1021 ggaacgagta atcgcccttg cgattgttta tcatgctgta aattgttata gatgcttcca
     1081 gctttttctt tttggatatt ctctatagcc atctcggtta atacctccat agctactttg
     1141 ttgtcgggat attttttatc aaagattata tgagtgcgtt gtgcataata ggtttggtcc
     1201 agagcatgca cccttctgaa tacaggtttt gccaataaat cacctgtttc atcacctgat
     1261 agagcatacc acccgccgct tgatgttaaa gcacagaaag ctattttttc acgcattctt
     1321 gtttctactt ctttgattgt tgattcatta ccgcttagaa tctcattttg aatatctttc
     1381 acaaaagaat agcgcaggac tgactctaaa gcttcgtcat aatcatagct cctttcatgt
     1441 ttaaattttt cttgcgcacc gtgcaaagtg tgatgatttg ccaaacgatg ttctgccatt
     1501 tcttccaata ttgtcatggc taaggagtta tgatcatgat taccggagaa agcaatacat
     1561 ccgcctttgg catggggatg caatttcttt tgtttatcca ggaataccac ttgagcctta
     1621 actccctcct cctccttaca ccatcctggt aaaccaccat aactatctct ttgcgcataa
     1681 taggtaccgc gagtaatatg ggttggccct ttttctggaa gcaacaaacc atcaaccaca
     1741 tcggtaagct tctgactccc actgaaaaga agctcatcta atataacggg tttaggttta
     1801 ttaaactgct ccgcaagatc ataaatataa gcataaattt gtttgcgtaa ttctggattt
     1861 aaatagccta cagcatcaat gcgaacaccc tcaaagccat aatcaatcat gtatttataa
     1921 atataaggct tccaaaattg ttcaataatt tcatctcgga tttgaggatc actgtagtca
     1981 aatgcgatag catccttaaa atcaggatgg acatcctttt gaaaccattc aggatgttct
     2041 tttctgtgcc gtgaatcggc ggcaacgtga tttaaaacca aatcaaacat cggtaccaaa
     2101 ccattattgc gggcttctga tgtgaacttc tgcattacgt ccatatccag ctgctttttc
     2161 aacttaggat caacattggg agggagcggg ttaaaataag gacttatcaa atcacaatct
     2221 gtcatagcat atagacttcg tgtcacctca ttataagctc tgacgccaat tgtcttatca
     2281 cgcttaaata aaccctgaac atcccctgct gtttgtacag gatttatcca tacagcatta
     2341 aatcccatgg cctgtaattg aggaagatag tcaatcatct cctctatgct tccaaattga
     2401 gtaggaaaca tgttataaca acgtaaaatc ccattaccat ccaggattac actttcttcg
     2461 cgttctcttt tattgactat ctctaatttt gattctgcca taataatatg cccatatcat
     2521 tatcttttag ataaaatata gtacatatct caggactatg cctcttgagc ttaaaaatca
     2581 agcaaacata gccgctgtat aatcaatagg gaaagttatc ttgtaatttt tctcattttt
     2641 gaaggttatt gagcggctaa actgatattg gcagttttta aataaatcaa attgtgttaa
     2701 aatattacca tatttatatc accaattatt aacaaatatt ggtgtcaaga cttagacttt
     2761 aatctctaac atgtcttgaa cttaaaaatt ttgtggagga tttatgggca gctcctataa
     2821 tgattggctg aaatcgtcat atagcgaatt aaagaaaata aatatcgtcg aaagtctgat
     2881 aaaagaaaac aattttgcca gggcgaaaat gctcttaaat aatcttgatc tcactaccct
     2941 catcaaatat accgaattaa gtaaaacaat tacagatttt tgtgaagaag cagagcaagc
     3001 tgatatttgg cggactcatc tgcaaaattt taacgaagaa catttttcat ttgaagaata
     3061 cccgccactc acagtgtccc aattagtaaa agggatctat ttttatggac aagcagctga
     3121 atgccgggaa gaagaaggta aaccatttag tgataatgaa ttggagtttc ttaagaagag
     3181 tgctcatcaa cactgttttt atgcttataa ctctttatca acctgggctt atgaaaaata
     3241 caaaatgggt ttgaatgatt attctttatt aaccttgcac tatgctcaaa aggctagtca
     3301 atatcattgg actccaggat atttattatt ttataagact tgtttaaatt tagccatttt
     3361 atcgaacgcc ccttctttat cttaccagga agcattagaa gcgctcttga ttgcccgaaa
     3421 attatctgag caccaatatt caataagtgc tatcaataat gcctactttg gcaagggcct
     3481 gattcatggc aataaaacgc agatagagtc ttgggataaa gcaatatcag aaactattgc
     3541 caaaggcaaa atcccctcta ctctgcttaa taaaatttac gataaagcat ctgaaaaagc
     3601 caaagggata ttggatgaat tcacagtcga agtaaaagat atgccagaag aagaaaaatt
     3661 ggaaaatgaa gtagctccta ctttgtatat ttaacgctat aatagcaagc gattcgactg
     3721 ataaaagtgg agttgtaacc aacaattcga tttttctcag ttttgtagtt taataatttt
     3781 ctacggtgat cgattctatg ggatatcatt ttttagcaga aggaaatggg attattgcat
     3841 gttatgacat gtacccaact cagtttcatt ctattaagga catgattaat tatttgcctg
     3901 tcttaaagca gatggggttt aatgccttat ggattaatcc catgcaaatg cccggtgaca
     3961 ttagcggctt ttttaaaacg gataaaaata atggtgtaaa aacaggaaat gaggtcacta
     4021 gaagcttata tgccatgagt cacccgttat tatttaatcc tcaattcagt ttggactccc
     4081 ctgaagaccc catggagaca acccaacgat taaactcaga agctttacag ttattcactc
     4141 aaaatgcaag gaacctcgga atagttccaa tgtttgatct ggttttaaat catgtcgcca
     4201 ttgattctcc cttgtgtcag gataagcctc attggttcaa aggagttcat gaagacttca
     4261 aagatgttcg cggcttcaat tatgatgatg aaagcataag aaaagaaata ataaccgaat
     4321 tttggcaacc ttatatcaaa cgttacatga tcgagtacgg ttttgatggt gttcgagtcg
     4381 atgctgtagg ttatgtacat cctgcagtaa gaagtgaagt atatgcctat attcactctc
     4441 ttgctgctga atatggtaaa ccaaatccgg ttatccttga tgaagcttta tttagtaaac
     4501 gaccacttgc ggatgaagta aattatttga aattacccgg cattggccca actcacatca
     4561 ctacagaagc ttataatgtt gaatttgatc ttgccagcgc tgatctgcct tatgaaataa
     4621 cattggaaga gcagttaaag gcgagtgtag tcttccaaca aaaagatgga acatggcgtg
     4681 aaaacacgaa ggggggctgc attaattttt gtgggaatca tgactatcgt tcattggcga
     4741 tgactatttt atttcaaatg gccaaaaaaa gactgaaatc tgacgctttt tataatgact
     4801 taatatcttc ctatcaagac ttgtacttta agtttaacgc cgagcaagaa aaaccacatg
     4861 aactcaagga gcctttgaaa acaaccttac tttactctta tgttgatcaa atcaaaaagg
     4921 agttggaaga taataaagat gaaacagcac aagaatttaa aactctggta ttagaaaaac
     4981 tggctttatc tgctcttaca gccagtggcg gatggttttt attatctggt gatgaaacat
     5041 gcgatatcac tgcaaaaaca gtatttcaga gaaaaggtgc tgttgataaa tcttattacc
     5101 cacaacgtga acatcgtatt tttagtgaaa aaccagttat tgctcacaaa attttagaaa
     5161 aaatggcaga agagaatttc ttcatagaaa actcggaaaa taagtggatg caggaacttt
     5221 accgaagttt atcaagtttt cctgaaatgc aaaaacgtct tttagtatct catattgata
     5281 atattaaaca ccaaataaat gccggcatag atcatgtaca agaaaaattt tccaggctac
     5341 ttgccagtga aagtttaaat ataggtttca catctcaaga ttttttagag aatccaagaa
     5401 cccacgaaaa tggatggctt ggattacagg ataattttct atttatcaaa caattaaata
     5461 gattattaaa aacactacct gcaccactac ccggattctc cagtgagttg gttcgactcc
     5521 ctgaaaaacc gcatctcatt attatcgtaa gaaaaaatgg taatgaatta aatgctccaa
     5581 ttgatgttgt tgttgttaac cttgaacagg aaaaacgaca aacattaact aaagaagatt
     5641 ttcaattaat tgccaaaaac tattgtaatc aatatttttc taaaaataga ccgagtg
//
LOCUS       DAXLDT010000185         5688 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXLDT010000185 DAXLDT010000000
VERSION     DAXLDT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.185"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..2176)
                     /gene="vpdC"
                     /locus_tag="ACF2SP_002726"
     CDS             complement(<1..2176)
                     /gene="vpdC"
                     /locus_tag="ACF2SP_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VpdC"
                     /protein_id="HFD2360495.1"
                     /translation="MMTPQHVISQLLESDTKYPQNLDLLKKIIISAYLGRLRINGLPP
                     DNKIALGNYLFDDERMIFDFTRLSDSKRALFIEWLLEPHQKDKEHGFLSGVYVNEYRG
                     FTAEVALSWWGILKNWLFNNKSEHWKITDLGLSVNYQLTGIEMCQGKQGILIGFNQFL
                     VPPSGTKYRDPNDNQSEPLGNVKRVFITDKLVDELVGLNYKNLNYESVCKSPHPQSID
                     VTDPEARHKDMYDYRKMQRFNGVRPWYIRLWRWISKLFFGDTYDAKKTSTADDNLELL
                     YKTKTVKIYQRSKSKEILVTEKRPDITNLVFCGGGAKIFAHVGVWKALNEAGMKPTKF
                     AGSSAGAIMSLMCYLGYTADEISELFKHFKQEHLVQFDIDRNGLSDTHSLKTALDYAI
                     ANKVRQIVNKYKIPYPKGKITFSTLASLREQFPDCGIGKELIVTATNKRSGKTIYLSA
                     NRYPLLEVSEAVKISASFPVLYRDTLLDGEEHNDGGILSNFPTEVFFDDHSTLLESEF
                     GNNLKVLAVQFDNGIERNVIDRIMDKVYRENFVLNWIYSLLTGVSDPASGWERDRLKL
                     RQYALQTIIPETGKVSTTGFNVERQSQEELIQNGYQSTLDYLNVRYTRKNNSYVNKEI
                     MYSTFNSLGDLLAYCCYRGDEQWFNVVNNLIVQSTLPNRTALMKQSLELRKLYFNTYL
                     PSSPEKNRTACNESNTLTFFGNAVCQHHNHSQELENGNHDVLL"
     gene            complement(2363..3052)
                     /gene="pyrF"
                     /locus_tag="ACF2SP_002727"
     CDS             complement(2363..3052)
                     /gene="pyrF"
                     /locus_tag="ACF2SP_002727"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFD2360496.1"
                     /translation="MTPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQ
                     FVKELVRREFKVFLDLKFHDIPNTVAKACHSAAELGVWMINVHAIGGLRMLQAARESL
                     KTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
                     VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNP
                     HEVVSALLRDL"
     gene            complement(3053..4168)
                     /locus_tag="ACF2SP_002728"
     CDS             complement(3053..4168)
                     /locus_tag="ACF2SP_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFD2360497.1"
                     /translation="MQFIDLKTQYSLIENDILNSIKRVLNHGQYIMGPEIAELEKELA
                     TFVGVKHAIVNSSGTDALLMALMALDIQPGDEVITSPFSFFATAEVISLCHATPVFVD
                     IDPLTYNLDPEQLERAITKKTKAIMPVDLYGQCADYDVINAIATQYGIPVIEDAAQSF
                     GATYKGKYSCSLATIGCTSFFPSKPLGGYGDSGACFTNDDVLAQKLIEIRIHGQNARY
                     CHHRVGINGRMDTIQAAILLQKLKIFSNEILLRQKVAKRYDQMLSELVKTPYVHECNA
                     SVYAQYTIEVENRDVFAKSMQASGIPTAVHYPIAMHQQQALGYLNYKLGDFPNSEKAS
                     QHVISLPMHPYLQEDEQLKIVAAVRNALLVMNEEAMV"
     gene            complement(4432..5601)
                     /gene="lapB"
                     /locus_tag="ACF2SP_002729"
     CDS             complement(4432..5601)
                     /gene="lapB"
                     /locus_tag="ACF2SP_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFD2360498.1"
                     /translation="MINLWPLLLPAAAWSGWWLANRSNSNKDAHVHNRLSREYVVGLN
                     YLLNEQSDKAVDVFIKLLEVDSDTVETHLALGSLFRRRGEVDRAIRIHQNLIARPQLS
                     IQQRKEALMALGQDYMSAGVFDRAERIFLEVVELGGSRDTSSLQGLLAIYQQEKAWEK
                     ALDIIKKLEISTGASFHNQAAHYYCEMASQALKINAMDRAIYCIKQAMNVDPESVRAS
                     LMNATIEIKEGRYKQAIRSLKRVPQQDAEFLTEIIEPLVLCHKELDSMPDCIHYLEQT
                     LEKHPRASVIFVIADYLRQNKGMDIAIDFVADNLSKHPSIRGLNRLIYWHLESAHGKV
                     REKLQMLYDITSKFLDNKPIYRCGHCGFGGKHLHWHCPSCKQWGRMKPIHGLEGD"
BASE COUNT         1781 a         1168 c          883 g         1856 t
ORIGIN      
        1 ccagtaagac atcatggttt ccattttcaa gctcctggct gtgattatga tgctgacaaa
       61 ccgcattacc aaaaaaagtt aaagtattgc tttcattaca agctgttcta ttcttttcag
      121 gactagaagg tagatacgta ttaaaataaa gttttctaag ttcaagggat tgcttcatta
      181 aagcagtacg attaggtaag gtagattgca caattagatt attaacaaca ttaaaccatt
      241 gttcatctcc cctgtaacaa cagtaagcaa gcaaatctcc caaagaattg aaagtagaat
      301 acattatttc cttgttaaca tagctgttgt tttttctagt atatcttaca ttaagataat
      361 ccagagttga ttgataacca ttttggatca attcctcctg gctctgcctc tctacattaa
      421 atcctgttgt tgatactttc cctgtttcag gaattatagt ttgaagagca tattgtctta
      481 atttaaggcg gtctctttcc catccacttg caggatcact cactccagtc agtaagctat
      541 aaatccagtt aagaacgaaa ttctctcgat aaactttgtc cattattcta tcaataacat
      601 ttctttcaat cccattatca aattgaactg ccaatacttt taaattatta ccaaattcag
      661 actccaacag cgttgagtgg tcatcaaaaa aaacttcggt tggaaaatta cttaatattc
      721 caccatcatt atgctcttca ccatccagca gagtatctct atacaaaact ggaaagctcg
      781 cagaaatttt aactgcttca ctgacctcca ataatgggta acgatttgct gataaataaa
      841 tagtttttcc tgagcgttta tttgttgctg taacaattaa ttctttgcct attccacaat
      901 ctggaaattg ctctcttaga gaagctaaag tactaaaagt tatttttccc ttaggatatg
      961 gaattttata tttatttact atttgcctta ctttatttgc gatagcataa tccagcgccg
     1021 tctttaagga atgggtatcc gataatccat ttctgtctat atcaaattga actaaatgct
     1081 cttgtttgaa atgcttgaaa agttcactaa tttcatcagc agtataacct aggtagcaca
     1141 tcaaagacat gattgcaccg gcagagcttc ctgcaaattt ggttggcttc attcctgctt
     1201 cattgagagc cttccaaacc cccacgtgag caaaaatttt tgccccacct ccacaaaaaa
     1261 ccaaattagt aatatcaggt cgcttctctg tgaccaagat ctcttttgat ttggagcgct
     1321 gataaatttt aacagtttta gttttataaa gtaattccaa attgtcgtct gcagtgcttg
     1381 tttttttagc gtcgtatgta tcgccaaaaa atagtttaga tatccatctc caaagtctga
     1441 tataccaagg cctcacacca ttgaaacgtt gcatttttct ataatcatac atgtctttat
     1501 gacgagcttc tggatcagtt acatcaatag attgcggatg agggctttta cagacagact
     1561 cataattaag atttttataa ttgagtccaa caagttcatc aaccagttta tcagtaatga
     1621 atactctttt aacattacct aatggctcac tttggttatc attaggatct ctgtattttg
     1681 tacctgaagg tggtactaaa aactggttaa atccaattaa gataccctgc tttccttgac
     1741 acatttcaat accagtcaac tgataattga cagaaagtcc caaatccgtt attttccaat
     1801 gctcagattt attattaaat aaccagtttt ttaagatacc ccaccaactt aaagcaacct
     1861 ccgcagtgaa acctcgatat tcattcacgt aaacgccact taaaaaacca tgctctttat
     1921 ctttttgatg aggctcaagc aaccattcaa taaataaagc tctttttgaa tcgctcaatc
     1981 gagtgaaatc aaaaatcata cgttcatcat caaataaata atttccaagt gcaatcttgt
     2041 tatctggagg taatccatta attctgaggc gtcccagata agctgatatg attatttttt
     2101 ttaataaatc taaattctga gggtatttgg tatcagattc cagcagttga cttatgacgt
     2161 gctgtggggt catcagtaaa tccttacgcc taggtagtcc atagaataaa tatctgaaat
     2221 aatattgatt atttttaaat atttattaat catctttata acaattatca taaatttcta
     2281 tggaataaaa taggaatggt acaactaatt caaaaatgaa caaaattata ccatatgtat
     2341 aattttttaa aagtatgtgc catcaaagat cacgcaatag agctgagaca acctcatgag
     2401 gattgctcgc ttgcgtaatt ggtcttccaa taaccagaaa atcacttcct tctctgatgg
     2461 cttgttgtgg agtcattact cgcgattgat catcttttag attatttggc agtcttatgc
     2521 caggagtaac ggtaataaaa ttctcaccac acttctgttt tataatttta acttcatgtg
     2581 cagagcttac cacaccatca agcccagcct ctctggcaag catagccaga tgggttgctt
     2641 gctcaggcaa tgtattacta ataccaacac tggccagctc cccttcttca aaactggtca
     2701 gcacagtcac tgcaatcaac aaaggcctgt ctttaccata cgtttttaaa gattctctgg
     2761 cagcttgcaa cattcttaag ccaccgatgg catgaacatt tatcatccat acccctaatt
     2821 ctgcagcgga gtggcatgct ttagcaactg tattagggat atcgtgaaat tttaaatcta
     2881 aaaatacttt aaactctctt cttaccaatt cttttacaaa ctgaggccct aatagagtaa
     2941 aaagctcact tccaacctta agtgcgcaat gattcggatc cagtttgtca accaactgta
     3001 aagcgttatc ttgattatca aaatccaacg caacgattaa tttaggtgtc atttatacca
     3061 tggcctcttc attcatcaca agtaaagcat ttctaactgc tgcaactatt ttcaattgct
     3121 catcttcttg taaataaggg tgcataggca ggctaattac atgttgactt gctttctcag
     3181 agtttggaaa atctccaagt ttgtaattta aataaccaag tgcctgctgt tgatgcatag
     3241 caattgggta atgaacagct gtaggtatac ctgaggcttg cattgacttc gcaaatacat
     3301 ctctattctc aacctcaatg gtatactgag catacacact tgcattgcat tcatgcacat
     3361 aaggagtttt cactaactca gaaagcattt gatcataacg ttttgctact ttttgtcgca
     3421 atagaatttc attagaaaat attttcagct tctgaagtag tattgctgct tggatagtgt
     3481 ccattcgacc attaatccct acccgatgat ggcaatagcg ggcattttgt ccatgaattc
     3541 taatttcaat taatttttgg gctaaaacat catcattcgt aaaacaagct ccagaatcac
     3601 cataaccacc taaaggtttt gatgggaaaa aactggtaca gccaatagta gccagggaac
     3661 aagagtactt gcctttataa gtagctccaa aactctgcgc agcatcttca ataacaggaa
     3721 taccgtattg agttgcaatg gcattaatca catcataatc agcgcattgc ccatataaat
     3781 caactggcat aatagccttg gttttcttag tgattgctct ctctaactgc tcaggatcca
     3841 aattataagt taaaggatct atatctacaa agacgggtgt tgcatgacaa aggctaatca
     3901 cttctgctgt tgcaaagaaa ctaaatgggc ttgtaattac ttcatcccca ggctgaatat
     3961 ccagagccat caatgccatt aataaagcat ctgtaccact tgaattcaca atcgcatgct
     4021 tcactcctac aaaagtggct aactcttttt caagctctgc aatttcaggc cccataatat
     4081 attggccatg atttaatacc cttttaatgc tgtttaaaat atcattctca attaaagaat
     4141 attgtgtttt taagtcgata aattgcatta taatctcata attagtatat aaattgttta
     4201 gctataccta agatcaatac catgaagtta aggaataatc ttaatacaaa tctaaagttg
     4261 caataaactg atttgaacgt aaactgcttt ggcaaacgag aagacaaaac aaatcaaatt
     4321 aaattaaatt tcctattcga attgttttac ttaatttgca ataacaaatt tgaattacga
     4381 tactaagcta atatgcaact gttcattaac tttctgagag tatgtctcag gttaatcccc
     4441 ttccaaacca tggataggct tcattctacc ccactgttta caacttgggc aatgccaatg
     4501 taaatgttta ccaccaaaac cacaatgccc acatcgatat atgggcttat tatctaaaaa
     4561 cttgctagta atatcataaa gcatttgcag tttttcacgt acttttccat gagcagactc
     4621 caagtgccaa taaatcaaac gattcaaacc tcttatcgat ggatgtttgc ttaaattatc
     4681 agccacaaag tcaattgcaa tatccatacc cttattttgt cgcaaataat cagcaataac
     4741 gaaaattaca gaggctcgcg gatgtttttc caaagtttgc tcaagatagt gaatacaatc
     4801 cggcatggag tccagctctt tatgacaaag aactaatggt tcaatgattt cggttaaaaa
     4861 ctctgcatcc tgctgcggca ctctttttaa agatcgaata gcctgtttat agcggccttc
     4921 ttttatttca attgtagcat tcatcaaact ggctcttacg gactccggat caacattcat
     4981 tgcttgcttt atacaataaa ttgctctatc cattgcattg attttaagcg cttgactagc
     5041 catttcacag taataatgag ctgcttgatt gtggaaactg gctccggtag aaatttccag
     5101 tttcttaata atatctaaag ctttttccca agctttttct tgttggtaaa ttgctaataa
     5161 tccttgaaga ctgcttgtat ctctactccc gcccaactca acaacctcaa ggaatatacg
     5221 ttcagctcgg tcaaatactc cagcactcat ataatcttgc cctaaagcca ttagggcttc
     5281 ctttcgctgt tgaatactta attgaggtct ggcgattaaa ttttgatgaa tacgaatagc
     5341 cctgtctact tcccctctac gcctgaacaa gctacccagt gcaagatggg tttctactgt
     5401 atcgctatca acttctagaa gcttgataaa tacatcaacc gctttatcag attgttcatt
     5461 taataaataa ttaagtccaa ctacatattc tcgcgataag cgattgtgga catgagcatc
     5521 tttattggaa ttactgcggt tagccagcca ccaaccagac catgcggcag caggtaacaa
     5581 cagtggccat aaattaatca tctattaatt ttcctctctg cgtatcgagt ttccactaat
     5641 aataaaatta atgttgatct tgtaagggta ttgaacgtaa attcttaa
//
LOCUS       DAXLDT010000186         5627 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXLDT010000186 DAXLDT010000000
VERSION     DAXLDT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.186"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..2224)
                     /locus_tag="ACF2SP_002730"
     CDS             complement(<1..2224)
                     /locus_tag="ACF2SP_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HFD2360499.1"
                     /translation="MFAEIPKIQEQLHNVLEYSQSMTSLWMQSIDKSLEQTNKVTQSL
                     INLNQQQIYPYPNILNDAIEYTVDFMQRSILFWDIMRKRGNQYLKHEQEGQPPVLIFS
                     YNMLMDGRHFERPVNYALVEIIPPEGIQIDPAKRPYVIVDPRAGHGAGISGFKDASQV
                     GLALRAGHPVYVIIFFPVPEPHQTLVDVTAAHEKFLTEVALRHPQSPKPCLIGNCQGG
                     WAILTLMAANPDVAGVAVVNGAPLSYWGGENGKNPMRYIGGIWGGSWIAQMAGDLGNG
                     KFDGANLVMNFEMANPKVTYWSKYYNLFANLDKEEIRFLNFERWWGGFSLLNVNEMRG
                     IIDNLFIGNKLVHGKIPLGQSGNNLDLRNISVPVIIFCSEGDNITPPQQALNWVSDLY
                     SNTLEIKLDGQVIVYLIHKSVGHLGIFVSSEVAKKEHNQIIDLLNYIEHLAPGLYELK
                     LNEIKENPKLPPHYLAYIEERSINDIRKGQENNVAIFNLVRMVSEYNAMSYDLFMAPI
                     IRNISNEYTAELIRKLHPLRQNQYLLNDLNPLVHPVSWISPFIRQNRIKISENNPFLT
                     QQLNFSKLINALWDFFSASRDNTVELMFYAVYGYIQLLAPFDPRRDMIVHSPEKDNQE
                     KMTQSIIAHISDGGVPEAILRILLLLVKIQGYIIGANLPDVIQKLRECNALKHLDKNG
                     FKQIVHSQTILIEYDPELAFNTIPHLLKSQEERSMVIQFIEDILKSLKVSPSEEYKKK
                     F"
     gene            2751..4199
                     /locus_tag="ACF2SP_002731"
     CDS             2751..4199
                     /locus_tag="ACF2SP_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2160 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360500.1"
                     /translation="MFLANQSLDFAWLSMQMFLINLLKDKIMRYAVTYCAMDHEFSGN
                     PFWHSCILLSQWDDNGKIEVVDNWGFYGVPSTVRDTWLSKLKIKLGLDVDLKGNHGML
                     RHEELRYLDVGYGLHGVTFELTKEKFDLLQLKCQTMLEEQKQAVKEVVESQGIAGKQK
                     YRIYEHEEYSPLIFALEKIKAKQKGHQPRLKPFELNLSFTLWGPALNQSYTCKSLMVD
                     LLKGILTQELIARITEAGKHPTVPRYSGKLERIYLHSSGPLREHKKSSGAIVHYRDLQ
                     DEGVKLHWTLPPQEIEALSGDTIKLLEISEEYCDEVKQAISKLQKLEWLFIDAKLPEK
                     YKPYQDDLVARIRQCYEAFAQVELKKAKSTATGWMGFAFSLLSLPRDLDEQCLLQKLN
                     NAKYLINSLYMAIVDHWKIYDDWPSETKEDSTDYNPLEALAAYLCEEDKKKLCKIIGR
                     NYLEPSSEEEFDEIEESGWTNSELGSTSARTM"
     gene            4485..5606
                     /locus_tag="ACF2SP_002732"
     CDS             4485..5606
                     /locus_tag="ACF2SP_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFD2360501.1"
                     /translation="MNALTSWIKAWSQKFFPNQKKNENKEEEADLTQEKKVRPNSDFY
                     ENYFSLVAAGTRLKLIEAMFGVNLFSLFENKAWVFEKDIIEKLQLMPIRARKWLHLLS
                     SEHFLIQIKVNGQQAYQLPEEFIELIHSEEFPAMEMFFKTWLVSAEENLTDVLRFGKV
                     KTSVSWPPKTQQEAEWLEDWMTRTADQPTYCLLQSINFKKVNKLLDVGGGDGTMACTF
                     VKKYPHLKAAVYNLPMSAQMARKNIESRKLNHRVHVIEGDFIEEDAFPVGYDLILFTR
                     VLFDWDDQVCRKLLRMAYQALPKKGLVGICEFYKEDNNDICLASEYRYIFHDDFGVNV
                     MKRASEYQNMLEKIGFTIVQPHKVKGMHFYYCSLILARK"
BASE COUNT         1701 a          955 c         1147 g         1824 t
ORIGIN      
        1 gaaatttctt tttatattct tcggaagggg atacttttaa agattttaat atatcttcaa
       61 tgaattgaat gaccattgag cgctcttctt gtgatttcag taaatgaggt attgtattaa
      121 atgcaagttc aggatcatac tctatcaata tggtttgtga gtgcacaatt tgtttgaatc
      181 catttttatc taaatgttta agagcattgc attcacgtag tttttgaatg acatcaggta
      241 agttagcgcc aatgatatag ccttgaattt tcactaataa tagtaatata cgcaaaatgg
      301 cctctggtac acctccatca ctaatatgag caataattga ttgagtcatt ttttcctgat
      361 tatctttttc aggggaatga acaatcatat cccttctagg atcaaatgga gctaataatt
      421 gaatgtagcc atagacagca taaaacatta gttccacagt attatcacga gacgcgctga
      481 agaaatccca taatgcattt ataagctttg aaaaatttaa ttgctgagtt aaaaagggat
      541 tattttcaga aattttaatg cgattttgac gaataaaagg tgatatccat gatacagggt
      601 gcactaatgg attcagatca ttcaataaat actgattctg ccttagtggg tgtaattttc
      661 gaatcaattc tgcagtgtat tcgttactta tattacggat tatcggagcc ataaacaagt
      721 catagctcat ggcattgtat tcagatacca tccgaacaag gttgaagatt gctacattat
      781 tctcctgtcc tttcctgatg tcgttaatag agcgctcttc aatataagcg aggtaatgtg
      841 gtggtaactt gggattttct tttatttcat tcagttttag ttcatataaa cccggagcca
      901 aatgttctat gtaatttaat aaatcaataa tctggttatg ttctttttta gccacctcac
      961 ttgagacaaa aatacctaaa tgaccaacac tcttatggat aagataaaca ataacttgcc
     1021 catccaattt gatttccaga gtatttgaat aaagatcaga aacccagttt aaggcttgtt
     1081 gaggtggtgt aatgttgtcg ccttccgagc agaaaataat aacaggcacg ctaatgtttc
     1141 ttaaatccag gttattacct gattgcccca agggtatttt gccatggacc agtttgttac
     1201 caataaacaa attatcaatt attcctctca tttcgttcac attcaaaaga gaaaaacctc
     1261 cccaccatcg ctcaaagttt aaaaatcgga tttcttcttt atcaagattg gcaaagagat
     1321 tgtaatattt gctccagtaa gtcactttag gattagccat ttcgaagttc attaccaaat
     1381 tagccccgtc aaatttgccg ttacccaaat caccagccat ctgtgcgatc cagctccctc
     1441 cccaaattcc tccgatgtag cgcatgggat tttttccatt ttccccaccc caataggaaa
     1501 gaggagcgcc attgactaca gcgacccctg ctacatctgg gttagcggcc ataagggtta
     1561 aaatagccca tcctccctga caattaccaa ttaagcaggg tttgggactt tgagggtgac
     1621 gcaaggctac ttcagttaaa aatttttcat gggctgcggt gacatccacc agcgtttgat
     1681 ggggttcagg aacagggaaa aagataataa cgtaaacggg gtgtcctgcc cttaaggcaa
     1741 gtcctacttg tgacgcgtct ttaaaaccgc tgattcctgc gccatggccc gcccgcggat
     1801 caacaatcac ataaggcctt tttgctgggt caatctggat gccttcaggg gggattattt
     1861 ccaccagtgc ataatttaca ggtctctcaa aatgcctgcc atccatgagc atattataag
     1921 aaaaaatgag aacaggaggt tgcccttctt gttcatgctt gagatattga ttgccccgtt
     1981 tgcgcatgat gtcccaaaac agaatgcttc tctgcatgaa gtctacggta tattcaatgg
     2041 catcatttaa tatgttggga taaggataaa tttgttgctg atttaagttg attaaagatt
     2101 gcgttacctt gtttgtttgt tcaaggcttt tatcaattga ttgcatccaa agagaagtca
     2161 tggactggga atattcaaga acattatgga gttgctcttg aattttgggt atttcggcaa
     2221 acatcttttt ctcctgtggc ctctcaattc gaggttcttt aactccatat cgttaacaaa
     2281 tgttgactat catggtacgt tgccatcatt ctcttctttt ttcatatttt cgtaaggcgg
     2341 ttttctcttt agagaaatat cctcttcaaa cgataataaa accattcgcc ttttttagcc
     2401 ttttaacacg ttccggtttg ctggccagtg caaataggag gcattgtgca tcgagcatgt
     2461 aaacccattt gttaatcact tttaaatcat tatagacagt tattgtcaaa acagtaaatg
     2521 caccgtgttc actgaaattc gattgttacg ctttggattt gtatcaattt tgtttttagt
     2581 ttgaaaggtt aaaaatttat ttctactaat ttatatgtta ggcagttggg tttgctttta
     2641 ttgggaaatt ccaggggtta tctcattatc tgtttagccg ttgacggcaa tatggggatc
     2701 tgtaatagtt tcataggatt gtattatgtg agtcaggttt aagcagttca ctgttcttag
     2761 ctaatcaatc tctagatttt gcctggttat ccatgcagat gtttttaatc aatcttctta
     2821 aggataaaat aatgagatat gcagttacct attgtgcgat ggatcatgag tttagtggta
     2881 atcctttttg gcatagttgc attttattat ctcaatggga tgacaatgga aagattgagg
     2941 tagtcgataa ttggggtttt tatggggtgc catctactgt tcgtgatacg tggttaagta
     3001 aactgaaaat caagttgggt ctggatgttg atttgaaggg caatcatggt atgttacgac
     3061 acgaagaact gcgttatttg gatgttggct atggtttaca tggagttact tttgagttaa
     3121 ccaaggaaaa atttgattta ttgcagttga aatgccaaac aatgttggaa gaacagaagc
     3181 aagcagttaa ggaagttgtt gaatcacagg ggattgctgg taaacaaaaa taccgtattt
     3241 atgagcatga agagtattcc ccgttaattt ttgctttgga aaaaatcaag gcaaaacaaa
     3301 aaggccatca gccaaggctt aaaccatttg agttaaattt gtcatttacc ctgtgggggc
     3361 cggctttaaa tcaatcctat acttgtaaat cacttatggt tgatttatta aaaggcatac
     3421 tgactcaaga gctaattgcc cgaattactg aagcaggaaa acaccctacc gtaccaagat
     3481 acagtggtaa attggaaaga atttatttgc atagcagcgg accattacgt gaacataaaa
     3541 aatcttccgg tgcgattgtt cattatcgtg atttgcagga cgagggagtc aaattacatt
     3601 ggacattgcc tccacaagaa attgaagcgt tatccggaga taccatcaaa ttactggaaa
     3661 taagcgagga gtattgtgat gaggtaaaac aagcgataag caaactgcaa aaattggagt
     3721 ggttatttat tgatgccaaa ttaccagaga aatataagcc ttatcaagac gatttggttg
     3781 ctcgtattcg gcaatgttat gaggctttcg ctcaggttga acttaaaaag gctaagtcaa
     3841 cagccacagg ctggatggga ttcgcctttt cattgctctc tttgccaaga gatcttgatg
     3901 aacagtgtct tttgcagaaa ttaaataatg ctaaatatct gattaacagt ctatacatgg
     3961 ctattgttga tcattggaaa atttatgatg attggccatc tgaaactaaa gaagactcta
     4021 ctgactataa tcctcttgaa gctttggctg cttatttatg tgaagaggat aagaagaaac
     4081 tttgtaaaat tattggacga aattatctgg agccttcgtc agaagaggag tttgatgaga
     4141 ttgaagagag cggatggacg aattcagagt tagggagcac ttctgccaga actatgtaaa
     4201 aaagcttgac tgacagtcat ctttttaaag tagcaagtgc ggattatcac gctggatcat
     4261 atctgcattt gcatatttat cgttcccatt gtgttatttg aggaaaccag ctaaacgagt
     4321 ttttttctac aggcccccaa tctctttgtt aaaaaagagt ttaacttggt catttagttt
     4381 atctaaactc cctcattaaa aacatttttt gccttgaccc tgggtttttt tgtaagtcct
     4441 gttctattct catattaata gtactcttaa aagaatattg tgggatgaac gctttaacat
     4501 catggataaa ggcatggtca cagaaatttt tccccaatca gaaaaaaaat gagaataaag
     4561 aggaagaggc tgatttaacc caagaaaaaa aggtgaggcc taattccgat ttttatgaaa
     4621 attatttttc tcttgtagcc gctggtacca gacttaaact gattgaagcc atgtttggtg
     4681 tcaatttatt ttctttattt gaaaataagg catgggtttt cgaaaaggat attatagaaa
     4741 aattgcaatt aatgccaatc agggccagga agtggctgca tcttcttagt tcggagcatt
     4801 ttttaataca aattaaggtt aatgggcagc aagcttatca attacccgag gaattcattg
     4861 agttaatcca cagtgaagaa ttccctgcca tggaaatgtt ttttaagacc tggcttgtta
     4921 gtgcagaaga aaacttgact gatgtattgc gctttggaaa agtcaagacg agcgtttcct
     4981 ggcctccgaa aactcagcaa gaagcagagt ggcttgagga ttggatgact agaacagctg
     5041 atcagcctac ttattgttta ttacaatcta tcaattttaa aaaggtcaat aaacttttgg
     5101 atgtgggtgg tggtgatgga actatggcgt gtacttttgt taaaaaatat ccacatttaa
     5161 aagcggcagt ctacaatttg ccaatgtctg cgcagatggc aagaaaaaat attgaatcga
     5221 ggaaactaaa ccatagagtg catgtgattg agggggattt tattgaggaa gatgcctttc
     5281 cggtagggta cgatctgatt ttatttacaa gagttctgtt tgattgggat gatcaggtgt
     5341 gtagaaaatt attaagaatg gcttatcagg ccttgccaaa gaagggatta gtcggtattt
     5401 gcgaatttta taaagaggat aacaacgaca tctgccttgc ctcggaatat cgttacattt
     5461 ttcacgatga ttttggtgtg aatgtgatga aaagggcctc agaatatcag aacatgttag
     5521 aaaaaattgg tttcactatc gttcaaccgc ataaagtaaa ggggatgcat ttttattatt
     5581 gttctttgat attggctcga aagtaaaaat tcttatcttt atttttg
//
LOCUS       DAXLDT010000187         5619 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXLDT010000187 DAXLDT010000000
VERSION     DAXLDT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.187"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            76..456
                     /locus_tag="ACF2SP_002733"
     CDS             76..456
                     /locus_tag="ACF2SP_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360502.1"
                     /translation="MFRYFKQGWHGDLKFSEVLFGSGGDYFLLEGGLAYIGFYILFAI
                     LLITSKPLSLDNILALALFSYGIVLYIWLIKAFWGSAHHCSNKISAVLIRTFTILLPL
                     ISIVLFFLIIIYYLVTAITDALSG"
     gene            510..1637
                     /gene="cfa"
                     /locus_tag="ACF2SP_002734"
     CDS             510..1637
                     /gene="cfa"
                     /locus_tag="ACF2SP_002734"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFD2360503.1"
                     /translation="MDIKKNKAKTFVNYMLQLANININGQTPWDIQIYNDEFYSRLLR
                     DADLGLGESYMEGWWDCQRIDLFISKLINANLESKIKINFKLAFNVFLSKILNLQTQK
                     RSLHVGRQHYDRGNDLFQIMLDSNMNYTCGYWRKAENLEQAQLNKLDLTCRKLYLKPG
                     MKLLDIGCGWGGLAKYAAENYGVSVVGITISQQQYDLAKTRCAHLPVEIRFQDYRDLN
                     EKFDRIVSLGMFEHVGYKNYRKYMEIVHQCLNDDGLFLLHTIGSNESVTKATPWISKY
                     IFPNGMIPSIMQIGQASEKLFVMEDWHNFGADYYKTLMAWHENFNQGWDQIKSQYSEK
                     FFRMWNYYLLSCAGAFDARMLQLWQIVFSKKGIHGGYSAPR"
     gene            1802..2128
                     /locus_tag="ACF2SP_002735"
     CDS             1802..2128
                     /locus_tag="ACF2SP_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360504.1"
                     /translation="MPKNNEPSGEELKKAGIVVGITNLLLWPFYYADSRWGLFASIFA
                     TSAVLYASHEVGESKRPVENSVARLGLFQQRGASNAEIENAFNNTVTGAAAIYDWLVP
                     GSNGPK"
     gene            2290..3741
                     /locus_tag="ACF2SP_002736"
     CDS             2290..3741
                     /locus_tag="ACF2SP_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HFD2360505.1"
                     /translation="MKFRTKLFLGLLLIVITTNGIFLFLSYQYGKETVYKEVGSSALS
                     IAAATAVLMNPEDIQKFNLDTSANSPLYKDLEKKLLEVRNANRRKDVYVNFIYSLFPD
                     SNNPKVLRFAVDPEENIIDKTELGTVMKLKTESGKTINVNYESPIVLPEFVEDIWGTW
                     LTALYPIKDAQGKYLGNIRVDVDAGSVNQRFHFLLYSGIAVFISTFLFAMFLGWLLFQ
                     WFNKPLLKITTALKNIAQGDLEQHLDIRTKDEFAEVGRVINEMTEGLRQRNMLQVSLT
                     RFISHALAEKIVKSGELPQVFSERRKVTIMVCDIRDFTTISERIKPELVVDFLNHFFE
                     KMIEAITSQHGILDKYLGDGFLAIFGSPDDDAYQEDHAVHAALKMREAMKSLNADWAK
                     ILGTEIQIGIGINTGSAIVGNIGTDIHMEYTAIGDTVNLASRIENATKKLNTDILISE
                     YTYIATDHTAFQFIELGEISIKGRVHKVKVYTL"
     gene            complement(3906..5465)
                     /gene="ravO"
                     /locus_tag="ACF2SP_002737"
     CDS             complement(3906..5465)
                     /gene="ravO"
                     /locus_tag="ACF2SP_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavO"
                     /protein_id="HFD2360506.1"
                     /translation="MPTGIVLSSQSPETTTSFPRRGGTAAGINHVTGFPIIKMHRDAQ
                     ARPDERKHFNQIKDGSLVFITGHSNTALDHLTGDYVPPLATPVQPMSTDWTYKQFRDL
                     ILKNGNLKAGDTITVVLWACNAGEGGLSSGAGLLGRLFREKQINTRVIASVATTMRFD
                     GHYSPTPEGAPAMAFITENGSKDIRIFDFSEEETIEWKNKGRLYVTSNGIEGYNLYSP
                     KQLESLESEKIYKVMEEIFVDRHYKENIRTGEDVKQYFSKSKMEFILRWSSANEADTE
                     NKYVFIAASFQAAGGIHSIRYGINKNGELFSINTTSNKVTPIDILPQGVMATLTQHIT
                     QNKELIEKALHEKGAQMTPSPRLKHGLKRKKSDHTELLLLKGEKEPLKSVQTDILSSK
                     VPASLRRSTQENQDFLMFVTPLQVQELLKSHGETKKEKHEKKIRLGHEKTIGDEKTRK
                     PVSKHKEPSGENEEPEMDLSKRQRIPVVQRKRPQGRRHEFFSDSPKNPDEHKTEKDKT
                     TLHSQNPTKGA"
BASE COUNT         1684 a         1035 c         1043 g         1857 t
ORIGIN      
        1 gtcctgaaaa attctttcat ccattgtcct agcttggctt ataataaaaa ttataaataa
       61 cataacagga tccgtatgtt tcgttacttt aaacagggat ggcatggaga tctgaaattt
      121 tctgaagttc tgtttggttc aggtggtgat tattttttgc ttgaaggcgg cctggcgtat
      181 attggattct atattttatt tgccattttg ttaataacat ctaaaccatt atccctagat
      241 aatatattag ccctagctct cttctcctat ggcatagtct tatacatctg gttaattaaa
      301 gcattttggg gatcggcgca tcattgctca aataaaattt cagcagtttt gattcgtacc
      361 tttaccattc ttttgccctt gatttcgatt gtgttgtttt ttctgattat tatttattat
      421 cttgttacag ccataacaga cgctttgagt ggctaatgat gttgcattca ttaaatgctt
      481 caacatcctt tagaaaaagg gagtttgata tggatattaa aaaaaacaaa gcaaaaacat
      541 ttgtcaatta catgcttcag ctagccaata tcaatatcaa tggccaaaca ccttgggata
      601 tacaaattta taatgatgag ttctattcca gattattgcg tgatgccgat ttgggtctgg
      661 gtgaaagtta catggaaggt tggtgggatt gtcaacgaat tgatttattt atttcaaaac
      721 tgattaacgc aaatcttgag tccaaaatta aaattaattt taaattggca tttaatgtct
      781 ttttatcaaa aatattgaac ctgcaaaccc aaaaacgttc tcttcatgtt ggtagacaac
      841 attatgatag aggaaatgat ctgtttcaga ttatgcttga tagcaacatg aactacactt
      901 gcggttattg gagaaaggct gaaaatttgg agcaagccca actcaacaag ctcgatctga
      961 cctgcagaaa actctacctt aaacccggta tgaaattgct ggatatcgga tgtggatggg
     1021 gaggacttgc aaaatatgct gctgaaaact atggggtatc agtagttggc ataacgatat
     1081 cacagcaaca atacgactta gctaaaacgc gctgtgctca tttgcctgtt gaaatccgat
     1141 ttcaggatta tagagactta aatgaaaaat tcgatcgtat agtctcgcta gggatgtttg
     1201 agcatgttgg ttacaagaat taccgaaaat acatggagat tgtccatcaa tgtttaaatg
     1261 atgatggttt gttcttatta cacactatcg gttccaatga aagtgtcaca aaagccacac
     1321 cctggatttc aaaatatatt tttcccaatg gaatgattcc atccatcatg caaataggcc
     1381 aagcttccga aaaattattc gttatggaag attggcataa ttttggagct gattattaca
     1441 aaacattaat ggcatggcat gagaatttca atcagggatg ggaccaaatt aaatcccaat
     1501 atagcgaaaa attctttcgt atgtggaatt attatttgct ctcttgtgct ggcgcttttg
     1561 atgccagaat gttgcaatta tggcagattg ttttttcaaa gaaaggaatt cacggaggtt
     1621 actctgcgcc gaggtaaatg gtttaaacga tttattctgc atcgtaatac cttcatcagc
     1681 aaacctggtt ttccatgcca tgaagttagc aagattattc atatatcccg ttaattttgc
     1741 ttgcaatttt tattatctgg aatatagttt gaactgaaat tttgtctaaa aaggaattaa
     1801 tatgccaaaa aataatgaac caagtggaga agagctcaaa aaagcaggga tagtggtagg
     1861 aataacaaac ctgcttttat ggccatttta ttatgccgac tcgagatggg ggttgtttgc
     1921 atccatattc gctacttccg cagtacttta tgcttctcac gaagtcggag aaagtaaacg
     1981 acccgtcgaa aattcagtgg cgcgacttgg tctcttccag cagcgtggag ctagcaatgc
     2041 tgaaattgaa aatgctttta ataatacggt cacaggtgcc gctgcgatat atgattggct
     2101 tgttcctggc tctaatggcc ccaaataata agcatcctgt cttataataa attatatttt
     2161 tgggaagtat tattttatcc aaaaccacat cgttctggag attggtaaag atctccagaa
     2221 atctgttgtc tttacttctc actgccttta tccaatactt ataattaatc tgctaatggt
     2281 ggataaataa tgaaatttcg tactaagtta tttcttggcc tacttctcat tgtaatcact
     2341 actaacggca tatttctgtt tttatcttat caatatggta aagaaaccgt atacaaagaa
     2401 gtaggctcat cagccctatc catagctgcg gcaacagctg tcctgatgaa tccggaggat
     2461 attcagaaat ttaatctgga tacgtctgca aactcacctc tttacaaaga tcttgaaaaa
     2521 aaacttcttg aagtgcgtaa cgcaaatcgt cgcaaagatg tgtatgtgaa tttcatatat
     2581 agtttatttc ccgactctaa taatcccaag gtacttcgct ttgccgtaga tcccgaagaa
     2641 aatattatag ataaaactga attgggaact gtcatgaaac ttaagactga atcagggaag
     2701 acaattaatg taaattatga atcccctatc gttctgcctg aatttgttga agacatctgg
     2761 ggcacatggc tgactgcttt atacccaata aaagacgcac aagggaaata ccttggaaat
     2821 atccgagtgg acgtagacgc cggttcggtt aaccaaagat ttcatttctt gctgtactca
     2881 ggtattgccg tctttatctc aacattttta tttgccatgt ttttaggatg gttacttttt
     2941 cagtggttca ataaaccctt attaaaaatt acaaccgcac tcaaaaatat tgcccaaggt
     3001 gatcttgaac aacatctcga cattaggaca aaagatgaat tcgcggaagt gggtcgagta
     3061 atcaatgaaa tgactgaagg attgagacag cgtaacatgt tgcaagtaag cttaacgcgt
     3121 tttatttctc atgctttagc agaaaaaatt gtgaaatccg gtgagttacc gcaagtattt
     3181 agcgagagac gtaaagtcac aattatggtc tgtgatatac gcgatttcac aaccatttcg
     3241 gagcgtataa aacctgaatt ggtagttgat tttttaaatc atttttttga gaaaatgata
     3301 gaagccatta cctcgcaaca tggaatattg gataaatatc ttggagatgg ttttttggca
     3361 attttcggtt cacctgatga tgacgcctat caagaagacc atgctgtcca tgccgcatta
     3421 aaaatgcgag aagcgatgaa atcactcaat gccgattggg caaaaatatt aggcactgaa
     3481 atacaaattg gtattggtat caatacaggc agcgccattg tcgggaatat tggaactgat
     3541 atacatatgg aatacacagc tattggcgat actgtcaatt tggcgtcacg aatagaaaat
     3601 gccaccaaaa aactcaatac agatattcta atcagtgaat atacttatat tgcgacagat
     3661 catacggcat ttcaatttat agagttagga gaaatttcta tcaaaggaag agttcataaa
     3721 gtaaaagttt atactcttta aaattaattg tcctctgact tgcttaatca cccgctcctt
     3781 ttaagacaaa aagcacacac tagctgcctg tttaagttag aaaatatgtg taacatatca
     3841 gcgactaact tgtgttttgg tgtaatgtag ttttgatgag catacaacaa aactaagctg
     3901 accagctaag cccctttggt tggattttgc gagtgcaatg tcgttttatc cttttctgtt
     3961 ttatgctcat ctggattttt tggggaatca ctaaagaact cgtgcctacg accctgaggt
     4021 cgttttctct gtacaacagg tattcgttgc cttttactta aatccatttc tggttcttca
     4081 ttttctccag atggttcttt atgtttagat actggctttc ttgttttctc atcaccaata
     4141 gttttttcat gacctaatct gatttttttc tcatgttttt ccttttttgt ttctccatga
     4201 gatttaagca attcctggac ttgcaacggt gtaacaaaca ttaagaaatc ctgattttct
     4261 tgggttgacc gccttagaga agcaggcacc ttgcttgaga ggatatctgt ttgaacggat
     4321 tttaatggtt ctttctcgcc ttttaaaaga agcaattcag tatgatctga ttttttacgt
     4381 ttcaatccat gttttagtct tggactggga gtcatttgag ctcctttttc atgaagcgct
     4441 ttctctatca attctttatt ttgagtaata tgttgcgtca aagtagccat tacaccctgt
     4501 ggcaaaatat caattggcgt aactttattg ctagtcgtat ttattgaaaa aagttctcca
     4561 ttcttattta ttccatatcg aatgctatga ataccaccag cagcttgaaa agatgctgca
     4621 atgaagacat acttattttc agtatcagcc tcatttgccg aactccaacg taatataaac
     4681 tccatcttag acttggaaaa atattgtttc acatcctctc cagttctgat attttccttg
     4741 tagtgcctgt caacaaatat ttcctccatc actttatata ttttttcact ttccagggat
     4801 tctaattgtt taggactgta caagttatag ccttcaatcc cattactggt aacataaaga
     4861 cgccctttat ttttccattc aatcgtttct tcctcagaaa aatcaaaaat gcggatatct
     4921 ttgctaccat tctctgttat aaatgccata gctggcgctc cctctggagt tggagaatag
     4981 tgaccatcaa atcgcatagt agttgctact gaagcaataa ctcttgtatt aatttgtttt
     5041 tccctgaata agcgccccaa taagcctgcc ccactactca gacctccctc acctgcatta
     5101 catgcccaaa gaacgacagt tatcgtatca cctgccttaa ggtttccatt cttcagaatc
     5161 aggtctctaa actgtttata cgtccagtcg gtactcattg gctgaactgg agtcgctaat
     5221 gggggaacat aatcgccagt aagatgatcc aaagcagtat tgctatgtcc ggtaatgaaa
     5281 accaggctgc catctttaat ttgattaaag tgctttcgct cgtcaggacg agcctgagcg
     5341 tctctatgca ttttaataat aggaaaacca gtcacatggt ttattccagc agcagttcct
     5401 cctcgacgag gaaaagaagt tgtggtttca ggggattgtg aacttaatac gattccagta
     5461 ggcattagtc atactcaatt agtttatctt gaaaatttga gcaaaattat atgcaatcag
     5521 atttttatta gtacttcaaa gttgttttgc tttgctcaat tgcgagtgct ttataatgat
     5581 caacgggaat catcttgatt ttgaaggaaa ttttccatt
//
LOCUS       DAXLDT010000188         5602 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXLDT010000188 DAXLDT010000000
VERSION     DAXLDT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.188"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(9..1214)
                     /gene="tyrS"
                     /locus_tag="ACF2SP_002738"
     CDS             complement(9..1214)
                     /gene="tyrS"
                     /locus_tag="ACF2SP_002738"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFD2360507.1"
                     /translation="MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTA
                     PDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAK
                     TYQHQVFKILDPDKTTVAFNSQWLNKFNAVDLIRLAATHTVARMLERDDFNKRYTTGQ
                     PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLI
                     EGLDGVKKMSKSLDNYIGINETPEQIFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQ
                     SVLEGKNPRDVKIDFAKEIVARFHDQTQAELAHNNFIERFQKGNIPEDLEELSLVIVE
                     PIALAQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRI
                     AKLSIQQAD"
     gene            1471..2904
                     /locus_tag="ACF2SP_002739"
     CDS             1471..2904
                     /locus_tag="ACF2SP_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFD2360508.1"
                     /translation="MDKRPNIPKRKSGNSSKLLAFFALVFAFSLPYLLVKNFSHPKRS
                     SYADQTDLYPELSDELSRDIPAEASTEIPHEKPDIKSATKPMQTKQDQQDKPINTSHV
                     KKDNEWQIVNPRSGDSMATIFHRLGLTAQNLNDVIKNNPHAKMLTRIKPNQKLQFLIK
                     KNKLEKLIVPVNTIQTLTVYRAGKIYKTKIDSKKTTTQNRYVTATVQGSLYTTAQRFN
                     IPSKLVRQMTTIFNKEIDFSRSLRSGDQFSIIYDAYYIEDKMVGVGDILVVTYTNRGK
                     TYQAIRHTNAHGEQDYFTPQGTSFKKAFTRYPIKFSHISSTFALSRYHPILHYRRAHK
                     GIDLAAPIGTPIHATGDGVITIIDRHNGYGNMIKIKHDKTYSTVYGHLLKFQKGLSKG
                     SRVKRGQVIGYVGQTGLATGPHCHYELHVNNQPRNPTTISLPTAAPVPAREMAAFKAK
                     ASTLLARLKLFEQAQLASKGKKKVNVG"
     gene            complement(3204..3737)
                     /locus_tag="ACF2SP_002740"
     CDS             complement(3204..3737)
                     /locus_tag="ACF2SP_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HFD2360509.1"
                     /translation="MNGFANHLSNWLFLTFIVGIPLYAAIKKVNVFDAFIIGAKQGFD
                     VSVKLIPYLVAMMVAIGMLRASGFFSLMSQILSPALAMIGMPSELLPLALIRPFSGSA
                     STGVMAELIHKYGGDSFIAKTAATMMGSTETTFYVIAVYFGSVGIRRTRHAIPAGLLA
                     DLAGIIASVLICRYLFT"
     gene            complement(3734..4339)
                     /locus_tag="ACF2SP_002741"
     CDS             complement(3734..4339)
                     /locus_tag="ACF2SP_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFD2360510.1"
                     /translation="MLNLIWLGMILISVVVGVIEGRIDEVVRAVTDSAKLGFELALGL
                     AGIMTFWLGIMSVATESGLVSILGKVLKPVLKRLFPDIPVEHPAMGAMVMNIAANMLG
                     LANAATPFGLQAMKELQSLNQNLKVATNSMCTFLAINTSSVQLIPVTAIAFLAANGST
                     NPSSVVFTSLIATTVSTLVAIVAVKQLAKLPRFKIERTDSL"
     gene            4409..5479
                     /locus_tag="ACF2SP_002742"
     CDS             4409..5479
                     /locus_tag="ACF2SP_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HFD2360511.1"
                     /translation="MKHTYNPFRYLIYFAFFITSLSSYSSQTTNIKEQTDTGLAPPAP
                     SVTTNSAAIEEQANSSIRKLYHTLNTMPNTSMVDRISQISAYFKGTKYILGSLGEGPN
                     ARYDQFPRYRVDGFDCDTYVNTVLSLALANSLESFQECLKHTRYKNGKRSYINRNHFT
                     SIDWNNYNQKRGLLKDITFSIRNEKKQPVALYANALINKPQWYNHKTIDTIRLQKQDK
                     NEQEKRLVELKAKGKTLETSLSNVPYIPFTALFSENKPNLHLFSQIPNGAVIEIIRPN
                     WDLRQQIGTELDISHLGFAIWINNELFFRQASSQYGKVVDVSLIDYLDKARSSPTIKG
                     INIQVVLPEKPVSNGCQLFDIR"
BASE COUNT         1892 a         1168 c          923 g         1619 t
ORIGIN      
        1 aaattatctc agtctgcttg ttgaatactc agcttggcaa ttcgacgttt tccaacttga
       61 ataatataag acttgccaat aggtaatttt agggaaggat ctgaaatttt atccccatcc
      121 actttaacag ccccttgttt caccattctt atagactctg atgtgctggc tgtcaaatca
      181 atttgcttta gtaattgtgc taaagctatc ggctctacaa taactaacga tagttcttcc
      241 aagtcttcag gaatatttcc tttttgaaat cgttcgataa aattgttatg ggcgagttcg
      301 gcctgagttt gatcatgaaa ccgtgcaaca atttctttgg caaaatcaat cttaacatct
      361 ctgggatttt tcccttctaa tacagattgc tttaattgtt gtatttcctt gcctgtttta
      421 aagcttaata aatcaatata tcgccacatt aactcatcag aaactgacat aatttttccg
      481 aagatttgct caggtgtttc attgatccca atgtaattat caagggattt cgacattttc
      541 tttacaccat ccaaaccctc tattaatggt gtcatcatga caacttgagg ttcaaagcca
      601 taatgcttct gtaattctct acccattaat aaattaaatt tctgatcagt accgccaagt
      661 tcaacgtcag cttttaatgc caccgaatca taaccttgta acagcgggta caaaaattcg
      721 tgaattgcga taggctgtcc cgtagtataa cgtttattga aatcatctcg ttccagcatt
      781 cttgccacag tatgtgttgc agccagtcga atcaaatcaa ccgcattaaa tttattaagc
      841 cattgtgaat tgaaagccac agtagtctta tcagggtcaa gaattttaaa tacctggtgt
      901 tggtaggttt ttgcattttc cagaaccgtt tcctgactca atggcattcg ggttacattt
      961 tttccagtcg gatcaccaat catggctgtg aaatcaccaa tcaaaaaaat aacctcatga
     1021 ccaaattgct gaaactgcct caacttatta agcagtacag tatgtcccaa atgaagatct
     1081 ggcgctgttg gatcaaaacc tgcctttatt ttcaatggga tacctttttg taatttcttc
     1141 tccaactcag gtacaggaag aatttcctca cacccccgca ttaattcgga acaaactgaa
     1201 tcttgaacta tcatgtcatt aaaacccgta taaatgattg acctatcttc tcgcagcgag
     1261 tatcttagcc cggttagcaa tttcctgcta taccaaatga tacattttga gataatttat
     1321 tactctaaat tgagaaaaat ataacaaaat atccaaataa gagtcgattt agttgactca
     1381 aggtagcggt cacttccaaa taatgactgt gatatattga ttgaaattta aatagcagca
     1441 tttttcagat gacggcaata taggcaaaag atggataaaa gaccgaatat accaaaaaga
     1501 aaatctggta actcatcaaa gctattagcg ttttttgcgt tggtatttgc cttttcgctt
     1561 ccgtatttgc tagtgaaaaa tttttcacac cctaaacgaa gcagttatgc tgaccaaact
     1621 gatttatacc ccgaactatc agacgagtta tcaagagata tacctgctga agcttccacg
     1681 gaaattccgc atgaaaaacc tgatattaaa tctgctacaa aacctatgca aacaaagcaa
     1741 gatcagcaag ataaaccaat caatacaagt catgtaaaaa aagataatga atggcaaata
     1801 gtcaatcctc gttcaggaga ttctatggcg accattttcc ataggctagg actaaccgcg
     1861 caaaatttaa atgatgtgat aaaaaataac ccgcacgcca agatgcttac aagaattaaa
     1921 cccaaccaga aattacaatt tttaattaaa aaaaataaat tggaaaaatt aattgtccct
     1981 gtaaacacca ttcaaacact aacagtttat cgcgcaggga aaatatacaa aaccaaaatt
     2041 gactcaaaaa aaacaaccac tcaaaatcgt tatgtgactg caacagtcca aggttctttg
     2101 tatacaacag cccaacgatt taacatcccc tcaaaattag tccgtcaaat gaccactatc
     2161 tttaacaaag aaattgattt ttccagatct ttgcgctccg gagatcaatt ctcgataatt
     2221 tatgatgctt attacattga agataaaatg gttggagtag gagacattct ggtagtaacg
     2281 tatactaatc gtggaaaaac ttatcaagca atacgtcata ctaacgctca tggtgagcaa
     2341 gactatttta ccccacaagg caccagcttt aaaaaagcct tcactcggta tccaattaaa
     2401 tttagccata taagttcgac ttttgcttta tcgagatacc acccaattct tcattacaga
     2461 cgagcgcaca aaggtatcga tttggctgcc ccgattggaa caccgataca tgctaccggc
     2521 gatggggtta ttacaatcat cgataggcat aacggatatg gcaatatgat taaaattaag
     2581 catgataaaa catacagcac tgtttatggg catttactca agtttcagaa aggtctatca
     2641 aaaggcagta gagtaaaacg aggtcaagta attggttatg taggacaaac cggtctggca
     2701 accggacctc attgccatta tgagcttcat gtcaataatc aaccaagaaa cccaactaca
     2761 ataagcttgc ctactgcagc accagtacca gcaagagaaa tggcggcatt caaagcaaaa
     2821 gccagtacac tattagcccg gctaaaactt ttcgagcaag cgcaattagc aagtaaaggg
     2881 aaaaagaaag ttaacgttgg ataaaaactg cgcgtgggat ttttcaatac ccaaatacta
     2941 atctaaatga cagcagacgc ttctttataa agtgagtaag ctccaagtaa ttcaaacata
     3001 atgttacttt tttgtgttaa aaaaaccgta aacgttcacg ggttatgcca tctcttgaaa
     3061 aacaaatgaa ataaatgctg tcattgcgaa cgaagtgaag caatccagaa cgatattttg
     3121 ttagaattct gtactggatt gcttcacttc gttcgcaatg acggaattta catgccccat
     3181 gaacgtttac aaaaaacctt atatcatgta aataaatatc gacaaatcaa aaccgaggca
     3241 atgattccag ctaaatctgc caataatccg gcaggaatag catgtcttgt tcttctgata
     3301 cccacagaac caaaataaac ggcaatcaca taaaaagtag tctccgtact tcccatcatt
     3361 gttgcggcgg tttttgcgat gaatgaatct cctccgtatt tatgaatcag ctctgccata
     3421 actcctgttg aagcacttcc agagaaaggt ctgataagag ctaaaggcaa taattcggaa
     3481 ggcattccta tcatagccaa cgctggcgat aagatctgac tcattaagga aaaaaaaccc
     3541 gaagctctta acatgccaat ggcaaccatc atcgcaacta aataaggtat aagctttacg
     3601 ctcacatcaa aaccttgttt tgctccaatt atgaaagcat caaatacatt tactttttta
     3661 attgcagcgt acaaagggat accaacgatg aaagtcaaaa acaaccaatt ggataaatga
     3721 ttagcgaatc cattcataag ctgtctgtcc tttcaatttt aaatcgcggt aatttagcca
     3781 attgttttac agcaacgata gctaccagag tagaaacagt agtcgcgatc aaagaagtga
     3841 agaccacact acttgggttt gtgctcccgt ttgcagcaag aaatgcgata gcagtgaccg
     3901 gaattaattg cacactggaa gtgtttattg ccaagaaagt acacatggaa ttcgtggcaa
     3961 ctttcaaatt ctgattcagg ctctgcaatt ctttcattgc ctgcaatcca aaaggggtcg
     4021 cggcattagc aagacccaac atgttcgcag caatattcat taccattgct cccattgcag
     4081 gatgctcaac aggtatatca ggaaataacc gctttaacac tggtttgagt actttaccta
     4141 aaatagaaac taaacctgat tcagtagcaa ccgacattat tcctaaccag aaagtcatta
     4201 tcccagccaa accaagcgct aactcaaatc ccagcttggc agagtcagta actgctctta
     4261 ccacctcgtc aatacgcccc tcaataactc caaccaccac tgaaatcagt atcattccta
     4321 accatattag attgagcatt attgcctcca acctctgaaa aaggttaata tgtaaccaaa
     4381 attagattta aaataccaat caggacttat gaaacatact tacaatccct tcagatactt
     4441 aatatatttc gcttttttta tcaccagcct ttccagttat agcagccaaa caaccaacat
     4501 taaggaacaa actgatacag gtttagcccc acccgcccct tcggttacca ctaactcagc
     4561 tgctattgaa gaacaggcta attcttcaat taggaaacta tatcacacac tcaataccat
     4621 gccgaacact tctatggtag atagaattag ccagatcagc gcatacttta aagggacaaa
     4681 atatattctt ggctctttgg gtgaaggtcc gaatgctcgt tatgatcaat ttcccagata
     4741 tcgagttgat ggctttgatt gtgataccta tgtcaataca gtactttctc tagcattagc
     4801 taactctctg gaatcattcc aggagtgttt aaaacatact cgctataaaa atggaaaaag
     4861 atcttatatc aacagaaatc attttacctc tatagactgg aataactaca atcagaagag
     4921 aggtttactt aaagacatta ccttctctat cagaaatgaa aaaaagcagc ctgttgctct
     4981 ttatgctaat gccttaatca ataaaccaca atggtataat cataaaacga tcgataccat
     5041 tcgattgcaa aaacaagata aaaatgagca ggaaaaacgt cttgtggaac taaaggccaa
     5101 gggtaaaacg ctggaaactt ctctttctaa tgtcccttat attccgttca ctgccttatt
     5161 ttcagagaac aagcctaacc tgcatttatt ttcccaaatt cctaacggag ctgttataga
     5221 aatcatcaga cctaattggg acttacgcca acaaataggg acagaattag atatttcgca
     5281 tttaggcttt gctatttgga ttaataacga actgtttttt cgacaagcct cttctcaata
     5341 tggtaaagta gttgatgtat cattaattga ttaccttgat aaagcacgca gtagcccaac
     5401 tataaaaggg attaatattc aagttgtatt gcctgaaaaa ccagtatcca atggatgcca
     5461 gctttttgat attcgatagc ttataaatta atatcttaat ggttaaccta taattttgcg
     5521 taattacgcc cgtataacca ttatttattt tatgaaatgt ccggagtttt tattaacgag
     5581 atactcgtgt ttcttaataa aa
//
LOCUS       DAXLDT010000189         5549 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXLDT010000189 DAXLDT010000000
VERSION     DAXLDT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.189"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..698
                     /locus_tag="ACF2SP_002743"
     CDS             <1..698
                     /locus_tag="ACF2SP_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HFD2360512.1"
                     /translation="IIYSECSGYVQGIDYNQIKKIAYDHHVQIFILIRAGDYVYKKSP
                     ILELYTDKSADYELLAPSIMLACVNINHARLLNQDIEFGFEQVAEIAVRALSPGINDP
                     YTARQSVFLLGELFLYIDQYDIEQDKLYYGKNIVGQYRGFTYKGIIDSALARLRQAAT
                     RDLTVILTIYDLVIQLSSLLNNKSMIKALLSHTHSLKEIINNEKLSKYDEEAFNEREK
                     QLTEIENSLSNSD"
     gene            1034..2296
                     /locus_tag="ACF2SP_002744"
     CDS             1034..2296
                     /locus_tag="ACF2SP_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1106 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360513.1"
                     /translation="MRNNKMGEKIPSFFHPDNKANSPQILIDVPSIIKDSELLDLLNA
                     AIDKDEKYHLLISCIRSHSPKYLNQRIMVKSHTTIEGDEYTPPHWTSLATKREYGYPT
                     DGNQNNELTTTLANIIATNELNFSSVQMVALFRELIIKEVDFTAIDQSPYLNLIHPKS
                     GNNPLKSLIAASLGDHEIIHLLHDFMSYIENKVPSDEQLLIINNQDNFEFEAMQPAEF
                     LLRIGHEDLALRLYRMGAQPTVQAFRLACSSYGTTPHYEEAMECIQFLMNSVHLDETD
                     LLEGKNSLQKASPAQYNEMLTRVLRSLQEDSKLDTGHSSLFEIIQKDYIENYQGKMFI
                     SFRDFCQKEAKTDHPLIQKYALLRIRQENVGNGLFSDYYRTESRHLAEKKWTLLNKNQ
                     RANQLTHDLIDLIDTKLGSLSTFPSARL"
     gene            complement(2395..2898)
                     /gene="msrB"
                     /locus_tag="ACF2SP_002745"
     CDS             complement(2395..2898)
                     /gene="msrB"
                     /locus_tag="ACF2SP_002745"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFD2360514.1"
                     /translation="MTKLISVVFYLFIIPNVMAYTPQFNKSEQLKKLTPLQYQVTQNG
                     ATEKSFDNAYWNNKEEGIYVDIVSGEPLFSSNDKYDSGTGWPSFTKPIDTSYLVFHTD
                     RSYFFIVRTEVRSKYANSHLGHVFKDGPEPTGLRYCMNSAALKFIPKNEMEKAGYGEY
                     LYLFNKK"
     gene            3096..4088
                     /locus_tag="ACF2SP_002746"
     CDS             3096..4088
                     /locus_tag="ACF2SP_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2360515.1"
                     /translation="MNQKQIKNQLALKWALEHLALNDEFKIIDQQKIIETAYSTVHRI
                     TSSESNVYYLKQTPEMLYLEPQTITYLQEQRCGNIPQIIAENKHLNCFLMLSCGDQTL
                     RNQFKENIDLEMLKTGILNYTKIQRLVENQLPQLIKLGIPDWRLEKFSSLYDQLIQQE
                     ELLLSDGLSKNEITLLNKAHEICFNLCESLSKYQIPETINHCDFHENNMILNQKTGNV
                     SIIDWGETVIAHPFFSLSGCLWNITYFYPIKQSDTIYEALQRQCITPWLGLHDETDLL
                     NALKIANQLNGVFAALSYKRIYDATMDQSQTVQQEHPGSIAGCLRSFLNHFHLA"
     gene            complement(4097..5296)
                     /locus_tag="ACF2SP_002747"
     CDS             complement(4097..5296)
                     /locus_tag="ACF2SP_002747"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HFD2360516.1"
                     /translation="MRLLKGILFTFCGIIFCLNSHAALEHEKLVNYISLGEFSRETAE
                     IALKKMPPLDTLTVHYDLQLYKINYKTQAPDGNLTIASGLVAMPIHPVGQVGIVSYQH
                     GTRFERNDVPSRNNEKNYIYLAAYGNSAGYMTVMPDYLGLGDNELTLHPYVQAETLAS
                     SSIDMLFAAKELANRLHYPISDKLYLAGYSEGGFSTIVMFEMLAKEYPALPVSAVAPG
                     SAPYGWEETMHFVMLEPGPRATAYLAYFFYSLQTYKSYWSGFDEIFAPPYNTLIPELM
                     DGYHAVAEILQALPQDPLLIFQPKFSNGIISKTDRNTEILKINFNHYDFKPTAPLLLV
                     GTKGDRDVPYAGAEMAYHSFRKYSDFVWIKSVSDTLDHVQAHPFVLKEQVDFFKQFER
                     QEAKTEF"
BASE COUNT         1903 a         1059 c          946 g         1641 t
ORIGIN      
        1 aaataattta ctctgaatgc agcggttatg ttcaaggcat tgattataat caaataaaaa
       61 aaatcgcata cgatcatcat gtgcaaatat ttattcttat aagagctgga gattatgtct
      121 ataaaaaaag tcccatacta gaattatata ctgacaaatc agcagattat gaactcttgg
      181 ctccatctat tatgctagcg tgcgtcaata taaatcatgc caggctctta aatcaggaca
      241 ttgaatttgg attcgagcaa gtcgcagaaa ttgctgtacg tgctttatct ccaggaatta
      301 atgaccctta cacagcaaga cagtctgtgt ttttattggg cgaattattc ctttatatcg
      361 atcaatatga cattgaacaa gacaaattat attacggtaa gaacatagtg ggtcaataca
      421 gaggcttcac ttataagggt ataatcgatt ccgccttggc acgattaaga caagcagcaa
      481 caagagatct gacagtcata ttgacgattt atgatttggt tattcaacta agcagcttat
      541 taaacaataa gtctatgatc aaagccttat taagccatac tcattcattg aaagaaatca
      601 ttaacaatga aaaattatca aaatatgatg aagaagcttt taatgagaga gaaaaacaac
      661 taaccgaaat agaaaattcc ctatccaact ctgattagaa taatcaagtt ttaattcgat
      721 tgacgatgga tattagatca actacacaca ggtataggtc tgaaaaactg aaacacggtt
      781 tgtccttata tatattatcc aggcaacttt gtaaaacact ctatgaattt ttcatcaagt
      841 tttaactctc cattttttca tcagatagca tgaaaatatt cttgacttat ataaataatc
      901 taatcaagat aaaatcatca tgactaaaat aggactaaaa acagatctat tgaccgttac
      961 aaacaaaaca tgggttcgaa gcttttgttg cattagctat cgcttttaaa ctttttgaaa
     1021 caggttctta actatgcgaa ataataaaat gggagaaaag attccaagtt tttttcatcc
     1081 tgataataaa gccaactcac ctcaaatatt gatagacgtc ccatcaatta ttaaagatag
     1141 cgaattgtta gacctgttaa acgccgctat tgacaaagat gagaaatatc acctcctaat
     1201 atcctgcatt cgcagtcaca gtcccaaata tttaaatcaa agaattatgg ttaaaagcca
     1261 tacaacgatt gaaggtgatg agtatactcc cccccactgg acatctttgg cgacaaaaag
     1321 agaatacggt tatccaaccg atgggaatca aaataacgaa ttgacaacaa ccctggcaaa
     1381 tatcattgct actaatgaat taaacttttc ttccgttcaa atggttgcct tgtttagaga
     1441 gttaataata aaggaagtgg attttaccgc catagatcaa tccccttacc tcaatttaat
     1501 ccatcccaaa tctggcaata accctctcaa aagcttgata gcagcaagtt tgggggatca
     1561 tgagattatc catttactgc atgatttcat gagttatatt gaaaataaag ttcctagtga
     1621 tgaacaatta ttgattatca ataatcaaga taattttgaa ttcgaagcga tgcaaccggc
     1681 tgaattttta ttacggatag gtcatgaaga tttggcttta cgcttataca ggatgggagc
     1741 tcaaccgact gtacaagcat tccgactggc ttgctccagt tatggcacca cccctcacta
     1801 cgaagaagcg atggaatgca ttcaattctt gatgaattct gttcatctgg atgaaacgga
     1861 tttattagaa gggaaaaatt ccttgcaaaa agcaagcccg gcacaataca acgagatgtt
     1921 gacaagagta ttacgctcct tgcaggaaga ttcgaaactt gatactggac actcctctct
     1981 ctttgaaata atacagaaag actacataga aaattatcag ggtaaaatgt ttatttcttt
     2041 tcgcgatttt tgtcagaaag aagcaaaaac tgatcatcca ctgattcaaa aatatgccct
     2101 tttgcgcatc aggcaagaaa acgtaggtaa tggcctgttc tcagattatt accgcactga
     2161 atccagacat ttggccgaga agaaatggac tttactcaat aaaaatcaaa gagccaacca
     2221 attaacacat gatctcattg atttaattga cactaaactt ggatcattaa gcacgttccc
     2281 ttcagccagg ctataatttg aggaacatac ggcaagttca gcccatttaa gctctaacat
     2341 tgatttgagc tcaatgagtt ccataggcta acttgctgaa aaaacaaggg cagtttattt
     2401 tttattgaac agataaagat actccccgta cccagctttt tccatttcat tttttggaat
     2461 aaatttcaag gcagctgaat tcatgcaata tcgcaacccg gttggttctg ggccgtcttt
     2521 aaagacatgg cctaaatgag agttagcgta tttagagcga acttcagtac gcacaataaa
     2581 aaaatagctt ctgtcggtat gaaaaactaa ataggatgta tcgattggct ttgtaaaact
     2641 gggccaaccc gttcctgaat cgtatttatc attagaacta aacaaaggtt ctcctgatac
     2701 tatatcaaca taaatacctt cctctttatt gttccagtaa gcattatcaa atgatttttc
     2761 cgttgcccca ttttgggtta cctgatattg caatggagtc agcttcttaa gctgctctga
     2821 tttgttaaat tgaggagtat aggccatcac atttggaatt ataaacaggt aaaatacaac
     2881 gctgattaat tttgtcatgt cataactcca catgcacttt catgcccaca ttgatgacgc
     2941 ttatttatag tgtagcaagc gcaccccata ttagcgaaga gagtaataaa tctgcgtttc
     3001 catcaaggat caaaacagaa gataagcatg atgtttggac ttaaactatt ttatacatta
     3061 aattcatatt aaagattatt agttatgagc agactgtgaa tcaaaagcaa ataaaaaatc
     3121 agctcgcctt aaaatgggct cttgagcatc tggcattaaa cgatgaattt aaaatcattg
     3181 atcaacagaa aattattgaa accgcttact caacagtaca tagaatcacc tcttccgaaa
     3241 gcaatgttta ttatctgaaa caaacaccgg aaatgttgta cctggagccc caaacaataa
     3301 cctatcttca ggaacagcga tgtggcaata ttccccaaat cattgcagaa aataaacact
     3361 taaattgctt tttgatgctg tcttgtggcg atcaaacttt acggaatcag tttaaagaaa
     3421 atattgattt ggaaatgctt aaaacaggta ttcttaatta cactaaaatt caacggttag
     3481 tagagaatca actcccacaa ctcataaaat tgggtatccc cgattggcgg cttgaaaaat
     3541 tttcatcatt atatgaccaa ctgatacaac aagaagagct tttattaagt gatggactat
     3601 caaaaaatga gattaccctc ctgaacaaag cacacgaaat ttgctttaat ttatgtgaat
     3661 cactctccaa ataccaaatt ccagaaacaa ttaatcattg tgattttcat gaaaataaca
     3721 tgattctcaa tcaaaagacg ggaaatgtaa gcatcattga ctggggagaa accgttatcg
     3781 ctcatccatt tttttctctg agcggttgct tgtggaacat aacttacttt tacccaataa
     3841 aacagtccga cacaatatat gaagcattac aacgacaatg tattactcct tggttaggtc
     3901 tgcatgacga aactgattta ttaaatgcgc taaaaattgc caaccaactc aatggggttt
     3961 ttgctgcatt aagttataag cgaatttatg acgcaactat ggatcaatct caaacagttc
     4021 aacaagaaca ccctggctcg atagccggct gcctaaggtc attcttaaat cactttcatt
     4081 tagcttaatt gttacatcaa aactctgttt tcgcttcttg acgttcaaac tgtttaaaaa
     4141 aatcaacctg ttctttgaga acaaaaggat gagcctgtac atgatccaag gtgtcactga
     4201 cagattttat ccacacaaaa tcactatatt tcctaaagga atgatatgcc atttcagctc
     4261 ctgcgtaagg tacatcacga tcacctttag tgccaaccaa taacaaagga gctgtaggtt
     4321 taaaatcata gtggttaaaa ttaattttta aaatctcggt gtttctatct gtcttactaa
     4381 taattccatt agagaatttt ggctgaaaaa taagtagcgg atcttgtggt aaggcttgaa
     4441 gaatttcggc aacagcatga tatccatcca tcaattccgg gataagagta ttgtaaggag
     4501 gagcaaaaat ttcgtcaaaa ccagaccaat aactcttata agtttgcaga gaataaaaga
     4561 aataggccaa ataggctgta gcgcgaggtc ctggttccaa catcacaaaa tgcatggttt
     4621 cttcccagcc ataaggagct gaacctgggg caaccgcaga gacaggtaaa gcaggatact
     4681 cttttgcaag catttcaaac attacaatag ttgagaatcc accctctgaa tagcctgcca
     4741 gatataactt atcacttatt gggtaatgca gcctgttggc taactcctta gcagcaaaca
     4801 acatatcaat gctactgctg gcaagagttt ctgcctgaac ataaggatga agtgttaatt
     4861 cattatcacc cagaccaaga taatctggca tcaccgtcat ataacctgca ctattaccat
     4921 aggcagcaag gtatatatag tttttttcat tatttctgga aggtacatca ttgcgttcaa
     4981 atcgggtgcc atgctgatag ctaacaattc ccacttgccc cacaggatga attggcattg
     5041 caactaatcc tgaggcaata gtcagatttc catctggtgc ctgggtttta taattaattt
     5101 tatataattg cagatcataa tgtactgtaa gagtgtctaa tggcggcatt ttttttaaag
     5161 cgatttctgc tgtttccctg gaaaattcac ccaaagaaat ataattaacc agcttctcat
     5221 gctccagagc agcatgagaa ttaaggcaaa aaataatccc gcaaaatgta aaaagaatcc
     5281 ctttcaataa acgcattgtt ttcataacca tccataaaaa atgtggataa ttacatgttt
     5341 aataaggaga atcaaatgaa gcagttactt gcattaatgc aaaaatataa gttgaatttg
     5401 atcaggcagt cacctcataa atggcaactg cccgatcaaa tttgagctac ttcattgcaa
     5461 gcttgggtga ttgtaaatct ttgagtgcct tactaaaaat ctcttccact gcccgttgcg
     5521 aatcagtttg tatcccggtt actttagta
//
LOCUS       DAXLDT010000190         5549 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXLDT010000190 DAXLDT010000000
VERSION     DAXLDT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.190"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2129
                     /locus_tag="ACF2SP_002748"
     CDS             <1..2129
                     /locus_tag="ACF2SP_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFD2360517.1"
                     /translation="HIDLGELAKNDSLLACLVLLTRYYQNPFSPPSLIARLPLINSKL
                     SVDMFSRAAARASLDSEVKTIKLEQITDSDIPLVLIKKNGEAVLLILEDDGKRAILDP
                     KQPDSRIDLNQIADELSGEVIFVEQQYKPSTRAHETLSKEQKNWFWKVVIKSWPLYSE
                     VLVASLLVNIFALVVPLFSMNVYDRVVPNHAIETMWVLASGVALVFLFDMLLKSLRSY
                     FIDVANKHSDKELSALIFQQVLGLSMANRPKSVGSLANTVQSFEVFRDFITSTTMTVL
                     VDLPFVFIFLFVIYLIGGVLFWIPFLVLPTIFIIGLLLQWPLIRLTRKSYQYGAEKQA
                     TLFESLATIETVKTTGAESVLQSRWERIINESSKNSMQLRFVANTSISITNLAQQIAT
                     IAIIIAGVYMISEGELTTGGLIACTILTGRALAPMAQVSTLFTRYFQSVNALDSLNKI
                     MQLETDTTEETRYLHRPSLKGAVEFKQVSFHYKEDETKVLQNLNFKINAGERVAIIGR
                     VGSGKSTLGKLILKLYVPNEGTILLDGTDYKQINPDDLRQQIGYVPQDIVLFYGSIRD
                     NICVGAPFINDSALLEAAEIAGVTSFTNNHPKGFDRQVGEKGAELSGGQRQSVALARA
                     LIMSPNILLLDEPTSAMDDNNEKQIKKNLNEYLSDNRTLILITHKISMLELVNRIIVL
                     ENGKIIADGNKEAVLSALRTGVTVRK"
     gene            2133..3269
                     /locus_tag="ACF2SP_002749"
     CDS             2133..3269
                     /locus_tag="ACF2SP_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFD2360518.1"
                     /translation="MKKTHSSKTFTHIILWTSLLFFIVAIIWANYAILDEVTTGQGKV
                     IPSSEVQVIQNLEGGIIQNIFVKEGQIVKKDQILMQIDNTRFMASYAEAEKKIDALEI
                     EIIRLNAEISDTKPVFPDKFIKTYPHLVQDQLSLYESRMRELNQLKKSLELAQKELDL
                     TRPLLKGGSVSEVEVIRLERSVSEIKGNIEKFKSEELDKLNKARTELFALVEANKADK
                     DRLTRTTVRSPVYGIVKQIKTTTIGGVVQPGSDLLEIVPLDDTLLIEAKIRPSDIGFI
                     HPGQKAMVKITAYDFSIYGGLEGVVEHISADTIIDEKTDKKEESYYIVKVRTEKNYLG
                     TEKKPLPIIPGMQATVDILTGQKSVLQYLLKPILKAKQSALRER"
     gene            3405..>5549
                     /locus_tag="ACF2SP_002750"
     CDS             3405..>5549
                     /locus_tag="ACF2SP_002750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFD2360519.1"
                     /translation="MPSIVLPKLSGSYYISLYLTIVFSTLTLLLGLSVIICWHSGIVF
                     LIQYYPNTVAIVYNTALCFIALSFSIFLFLNWHYKSAIILSLAVFILSGIALVQHAYS
                     INLGIDEIFFHHYQSIQNAYPGRMAANTAFCFLLIATALILMSFTGNQSLNNSLAGAL
                     SLLVFCLAVLFISGYFSDIQQAYKWGNKTPMSINTGIGFLLLSLAMMSLYWHNNTIHQ
                     LNSSIAMPYLSSFCIMLTFSLLSFEIFKKEKELNLSTNLSLTTFALGILFSILFGLII
                     RLWQLAKISTATAKYALSEVNATLESTADGILVVDRTGNVMNYNKRFLNMWYQKERLL
                     KTINYHDIKLMINKQLINRREAIQRINKILKNPDYQQAFELKLKNNTYYHCYTQPLKL
                     DDEIIGRVWSFRDITIPKRLEMELSHQSTHDTLTNLPNKVLAINILGYAMKTAVSSKK
                     IIGVYLLDLDRFTQINDVFGHSKGDEIIKSVSKRLIDCMPMNHVLGRLSGDQFIVIAS
                     INNSKEVVTGVTRLMSAFHEPVKMNGHSIKMSCSVGISFYPKDGDTVDTLLSKADIAL
                     SRTKIEGRNSFQFYTQEMHSYTLEHILLESELRNAVAKNEFVLYYQPILKLSTLSVIG
                     FEALIRWNHPGKGLILPDKFISLAEEIGVIGEIGDWVFVTVCKQIRAWQLQGFNNLKI
                     SINISPKQFKFRHFPQKVNELLSEFQVKPEFIE"
BASE COUNT         1794 a          911 c         1054 g         1790 t
ORIGIN      
        1 ctcatattga tttgggtgaa ctagccaaga atgactctct gctagcctgc ctggtattat
       61 taactcgata ttatcaaaat ccgttttcgc ctccatcact tatagctcgt ttgccattga
      121 tcaatagtaa attgagtgtt gatatgtttt cgcgtgccgc tgccagagcc tctttggatt
      181 ctgaagtgaa aactataaaa ttagaacaaa tcactgattc agatattccc ttagtattga
      241 ttaaaaaaaa tggcgaggct gtgcttctaa tactggagga tgatggtaaa agagcaatcc
      301 ttgatcccaa gcaacctgat tcccggattg atttgaacca aattgccgat gagttaagtg
      361 gtgaagtcat ttttgttgag caacaataca aacctagcac cagagcacat gaaactcttt
      421 ccaaagaaca aaaaaactgg ttttggaaag ttgttattaa atcttggcca ttgtattccg
      481 aagttcttgt ggcctctctg ctcgtgaata tatttgcact cgttgttcca ttatttagca
      541 tgaatgtgta tgaccgtgtg gttccaaacc atgctattga aaccatgtgg gtgcttgcta
      601 gtggtgtagc tttagtattt ctctttgata tgctgctcaa gagtttacga tcctatttca
      661 ttgatgtggc aaataaacac agcgataaag agttgtctgc tttaatattt cagcaagttc
      721 tcggattaag tatggctaac aggccaaaat cggttggttc tttagccaat acagtacagt
      781 catttgaagt attcagagat tttattactt cgactactat gacagttctt gttgatcttc
      841 cttttgtatt tatttttctt tttgttattt atttgattgg cggggtgttg ttttggatac
      901 cttttttagt gctccctacc atttttataa taggtttatt gctgcaatgg cctttaattc
      961 gacttacaag aaaatcctat caatatggtg ctgaaaaaca agcgacgttg tttgagtctt
     1021 tagctactat agaaactgta aagacaactg gagcagaatc agttctgcaa tcacggtggg
     1081 aaagaataat taatgaatct tctaaaaact ctatgcagtt acgttttgta gccaatacaa
     1141 gcatcagtat cactaatctg gctcaacaaa ttgcgactat cgctattatc attgcaggtg
     1201 tttatatgat ttctgaaggt gagttaacca caggtggatt aattgcctgt accatattaa
     1261 ctggaagagc attagcccct atggctcaag tatccactct atttacacgt tacttccaat
     1321 cagttaatgc tctggactca ttaaacaaga ttatgcaact tgagacagat accacagaag
     1381 aaactcgtta tctccacaga ccttctctga aaggtgccgt tgaatttaaa caggtgtctt
     1441 ttcattacaa ggaagatgag acgaaggtat tacaaaatct taattttaaa attaatgctg
     1501 gtgagcgcgt tgcaattata ggacgagttg gttcaggtaa atcgactttg ggaaaactaa
     1561 tattaaaact ttacgttcct aacgaaggaa ctattttgct ggatggcact gattataagc
     1621 aaattaatcc tgacgattta cgtcagcaaa taggctatgt acctcaggat attgttttat
     1681 tttatggaag cataagagat aacatttgtg taggtgctcc ctttataaac gactctgctc
     1741 ttttagaagc cgctgaaata gctggggtaa cgagttttac caataatcat cccaaaggct
     1801 ttgataggca agtaggagaa aaaggagccg aattatcagg aggacaaaga caatctgttg
     1861 ctcttgctcg tgctttgatt atgagcccta atatcttgtt attggatgag cctacttcgg
     1921 caatggatga taataacgaa aagcaaatca agaaaaatct taatgaatac ttatccgata
     1981 atcgtacttt gattttaata actcataaga tttccatgct ggagctggtc aatcgtatta
     2041 tcgttttgga aaatggcaaa atcattgctg atggtaataa agaagctgtt ctaagcgccc
     2101 ttaggactgg ggtaacggtg aggaaatagt ctatgaaaaa aactcacagc tcaaaaacct
     2161 ttacccatat tattttatgg acttccttgc ttttttttat tgtggcaatt atttgggcta
     2221 attatgccat tcttgatgaa gtaactacag gacaagggaa agtaattccc tctagtgagg
     2281 ttcaggtcat acagaatctt gaaggcggga ttattcagaa tatttttgtc aaagaagggc
     2341 aaatagttaa aaaagatcaa attttaatgc aaatcgataa tactcggttt atggccagct
     2401 acgccgaagc agagaaaaaa attgatgcat tggaaataga aattataagg cttaatgctg
     2461 aaattagtga tacaaaacct gtgtttccag ataaatttat caaaacctat cctcatcttg
     2521 tgcaggatca gttgtctctg tatgaatcac ggatgagaga gttgaatcaa ctgaaaaaat
     2581 ccctagagtt ggcgcaaaaa gaattggatt taactcgccc attattaaag ggtggatccg
     2641 tttcagaagt agaggttatt cgtctggaaa gatcagtaag tgaaattaaa ggaaacatcg
     2701 agaagttcaa atcagaagaa ctggataaat tgaataaagc cagaacagag ttgtttgcgc
     2761 tggttgaggc gaataaagcg gataaagatc gtttaacaag gaccacagtt cgttctccag
     2821 tttatggtat tgtaaaacaa attaagacga caacgattgg tggtgtagtt caaccaggta
     2881 gtgatttact tgagatagtc ccgcttgatg atactttatt aattgaagca aaaatacgcc
     2941 cttctgatat tggttttata catccaggtc aaaaagcgat ggttaaaatt actgcttatg
     3001 acttttctat ctatggtgga ttggagggtg ttgtagagca tattagtgcc gatactatta
     3061 ttgatgagaa aacagataaa aaagaagaaa gctattatat agtaaaagtg cgaacagaga
     3121 aaaactattt gggaaccgaa aaaaaaccat tgcctatcat tccaggtatg caagctacag
     3181 ttgatatact tacaggtcaa aaatctgttc ttcaatacct tttgaagccc atacttaagg
     3241 ctaagcaatc agcacttaga gagcgttaat tagtgtgtaa aaacattttg gaaatgctgt
     3301 gtgttacgat tctgtgagaa tgtcgtgagc gtgcgataaa taaatagtct attattatat
     3361 tgataacacg tttgcaagaa aatcactgcc agggatgtta tattatgcca tcaatagtac
     3421 tccctaaatt atctggttct tactacattt cactttattt aacgatagtt ttttccacac
     3481 taacactttt attgggactc tctgttatta tttgttggca tagcggtatt gtctttttaa
     3541 ttcaatacta cccaaataca gtagccattg tttataatac cgctttgtgt tttatcgcct
     3601 tatctttttc tattttttta tttctaaatt ggcattacaa gtcagcaatt atattgagtt
     3661 tagctgtttt cattttatct ggtatagctc tagttcaaca tgcttatagt attaatcttg
     3721 ggattgacga aatattcttt catcactacc agtccattca aaatgcttat cctggcagaa
     3781 tggctgcaaa cacggctttt tgctttttat taattgctac agcacttatc ttgatgagtt
     3841 tcactggtaa tcagtcactt aataattctc tggcaggggc acttagttta ttagtatttt
     3901 gtttggcagt attgttcata agtggttatt tttcagatat acaacaggct tacaaatggg
     3961 gcaacaaaac ccccatgtca atcaacacag gaattggttt tttgctgctc tctttagcga
     4021 tgatgagttt atattggcac aacaatacca tccatcaatt aaattctagt attgcaatgc
     4081 cctacttgag ctcattttgc attatgctta ctttttcatt gctatcattt gaaattttta
     4141 aaaaagaaaa agaactaaat ttaagcacta atttatcact cactacgttc gctttaggaa
     4201 ttcttttttc tattttattt ggattaatca ttagattatg gcaattggca aaaatatcaa
     4261 cagcaactgc caaatacgca ttatcagaag ttaacgcgac gctggaatca actgcagatg
     4321 gtattttggt agtagataga acaggtaacg taatgaatta caataaacgc tttttaaata
     4381 tgtggtatca aaaagagaga cttctgaaaa cgataaatta tcatgatata aagctgatga
     4441 ttaataaaca attaatcaat cgaagggaag caatccaaag aattaataag atcttgaaaa
     4501 atccagacta tcaacaggca tttgaattaa aacttaaaaa taatacatat taccactgct
     4561 atactcaacc actaaaattg gatgatgaaa tcattgggcg tgtttggagt tttagggata
     4621 ttactattcc taaaaggctt gaaatggagc tatcccatca atctacacat gacacactca
     4681 cgaatttacc caataaagta ttggctatta acatactggg atatgcaatg aaaacagctg
     4741 ttagtagtaa aaaaataatc ggggtttatt tattagattt agatcgattt acacaaatca
     4801 atgatgtatt tgggcacagt aagggagatg aaataataaa atctgtttca aaacggttaa
     4861 ttgattgtat gccgatgaac catgtgcttg gacgacttag tggtgatcag tttattgtaa
     4921 ttgccagtat taacaatagt aaagaggttg ttactggagt cacgcgatta atgagcgcat
     4981 ttcacgaacc cgttaaaatg aacggccaca gtattaaaat gagttgcagt gtggggattt
     5041 cgttttaccc taaagatgga gacacagtgg acacactatt atccaaggct gatatcgcac
     5101 tatctcgaac aaaaattgaa ggtcgtaata gttttcaatt ttatactcaa gaaatgcata
     5161 gctatacctt agaacacatt ttactggaaa gtgaattaag gaatgctgtg gcaaaaaatg
     5221 aatttgtgtt gtattaccaa ccaattctaa aattgagtac tttgagcgtg attggttttg
     5281 aagctctaat tcgctggaat caccctggaa aaggattaat tcttcctgac aaatttattt
     5341 ctcttgcaga agaaatagga gtaattggtg aaattggtga ttgggtgttt gtcactgtat
     5401 gcaaacagat aagggcatgg caactacaag ggtttaacaa tttaaaaatt tcaatcaata
     5461 tttcaccaaa acagtttaaa tttagacatt tcccccaaaa agttaatgaa ctattgtccg
     5521 agtttcaagt aaagccagaa tttatagaa
//
LOCUS       DAXLDT010000191         5434 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXLDT010000191 DAXLDT010000000
VERSION     DAXLDT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.191"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..942
                     /locus_tag="ACF2SP_002751"
     CDS             <1..942
                     /locus_tag="ACF2SP_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0257 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HFD2360520.1"
                     /translation="FLFILIYHYSYLFPFTNNAFVVANVRPVAANVKGYITNIYIKNE
                     QEVKKGQPLFTVFKAPYELAYRKAVSDVQEAKAQLLLFNKQVEKTKYLLQAQKELYEK
                     FRFDYEHNHSALRDHAVSKLTVNTMLKEKNAALSKLKALEKELEVNQQQVIVQKKKID
                     SLIAVMKNAKIDLDETTVYAKQNGAVQDMFVALGTPIKTRKPVFAIADTDTLFIQANF
                     NETDLRRVQPGDKVSIFPRMYFGSKIYHGVILSRNWAASRLETHRATQIQIVRNSESN
                     WFLLPQRLPVQIQITDYDPVHYPLSIGASVYVYIHTH"
     gene            1119..3062
                     /locus_tag="ACF2SP_002752"
     CDS             1119..3062
                     /locus_tag="ACF2SP_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360521.1"
                     /translation="MKIPFEGKWRATITKREAGFSQRVVVGGATSGNGSYNGVVGNSF
                     VFEDGWVELQWNNNAGSGWQESAVISSVGMTSPLVVVKFVSADDNFPNQRDGDYDDLQ
                     VRFEHLDAPFEVTQRPYALDRGSLVMFPDGIFDASQGIQYMGVQIKNTWFFDWQSNFP
                     ATGMKIGIAPVSRSALSAAGIVVIDSWTPQEQEGLGQIMDGGFVRVPDLKINEETTIY
                     FKLDVSNANPSKPEIGFVAQRDAYDPHYDEPTRIVDKKIFISRSSYNPATRELVTAIP
                     EGTLYLKLNKIAIDRKGATKAAQELKKCLKQHGGGIKGMSKWLHKLSRGFWQNSKDKE
                     RWCKEQGNDALRKLLKQLLDGKDIDICHLKAILDQCCERPGGDCECCPCGDGDGRPGG
                     WDDGTGVDGWCRVKPVFWLPLDFEYKVVPNPAYIGQFGPLAFEDPWWKVVLIILAIIL
                     AIASVIYDYVYASEDDQYIIGQVVRNGDAPINLVDAAVSNLNGSRGVDLGQLDAQGND
                     INNGSPITALDSIIQLNRTAAGSYFGTLDAVLGNVVWKSGATSATTRGFISTLGTFVS
                     IPYDEHDTLSGTVTYTNEVVISELAAIPQTLSDPGDSGSVWVDLATARPVALNYGSPT
                     DSSDNRAFANPIRSVIDVLNLRF"
     gene            3081..4865
                     /locus_tag="ACF2SP_002753"
     CDS             3081..4865
                     /locus_tag="ACF2SP_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360522.1"
                     /translation="MKREFFQKLDKILDIASEHKQMLLQYPDVVMVGVGPERRKGKIT
                     GEPAIIVTVRKKRSSSELKKRGEKPLPQTIKGIPVDVIELGKAVEAPEIIAVQEKAKK
                     TISKIGAKWLQTPNITAIGYGYKEKGGEIDFNTIAIQFFVKKKFPMAELQKQQISPIP
                     EKINGVSTDVIQLSPIRPAAASGSRGDRHDPLKGGVAIGVGSKPFHYGTYGATVFDRT
                     TGEQLFLSNQHVLDASPGTDTIQPSPVLLDDSVEVGFQLDVCLPIHFIRLDTPNTTVG
                     TVLAGAAVAAAVAAALSDEIDPTRRGQEATMPATGSKTLFETQKVKMDYPELPIPGTP
                     FKVKTAWDYERHTDAGIKMFSVSEEKKNPHVLRDKLLITDKKLYHPGDTIRIFGLILP
                     EECAPASQSRNDRQPINSRELEDITHYPNVASSINLDLVASMDSDIASSPARRTNTRA
                     AVINPKRCRCDRYHPVAILTPTTEDRAFPVVLREPVFAQKNQIFQDLLTVVKQRDDEK
                     LLERVFVLVRYGCIYTGTLRVNNIPAGPWKHFFYVQTVNIATSDMKPLLAAQIIGGLP
                     VSQNAKADLDIACGPLTWEDGEFDIELI"
     gene            5083..5434
                     /locus_tag="ACF2SP_002754"
                     /pseudo
     CDS             5083..5434
                     /locus_tag="ACF2SP_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212682.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1595 a         1036 c         1267 g         1536 t
ORIGIN      
        1 tttctgttca ttctgattta tcattactcg tatttatttc cttttacaaa caatgcattt
       61 gttgttgcga atgttcgtcc agtcgctgcc aatgtaaaag gttatatcac caatatttat
      121 attaaaaatg aacaggaagt aaaaaaaggc caaccattat ttaccgtatt caaagccccc
      181 tatgaattgg cctacaggaa agctgtgagc gatgttcaag aggcgaaagc ccaattattg
      241 ttattcaata aacaagttga gaaaacgaaa tatctgctgc aagcacaaaa agagctttat
      301 gaaaaattcc gctttgatta tgagcataat cattctgcct tgcgtgatca cgcagtttct
      361 aagctgaccg tcaatacgat gttaaaagaa aaaaatgcgg cactaagcaa attaaaagca
      421 ttggaaaaag agttggaggt caaccagcaa caagtcattg tgcaaaaaaa gaaaattgac
      481 tctttaattg cggtgatgaa gaatgccaaa atcgatttgg atgaaaccac ggtttatgcc
      541 aaacaaaatg gggcagttca ggatatgttt gttgcattgg gaacccccat caaaacccga
      601 aaaccagtct ttgccatagc ggataccgat accttgttta tccaggccaa tttcaacgaa
      661 acggatttaa gaagagtaca gccaggggat aaagtctcta ttttcccgag aatgtatttt
      721 ggatctaaaa tttatcatgg agtgattctt tccagaaact gggctgcaag ccgcctggaa
      781 actcatcggg cgacgcaaat acaaattgtt cgcaatagcg aaagcaactg gtttttatta
      841 cctcaacgcc tgccagtaca aattcaaata acagattatg acccggtgca ttacccgtta
      901 agtataggcg ctagcgtcta tgtgtatatc catactcatt aaaattatgg aaggtgttga
      961 aatcaagagt gcaatatatt ctgttttttt tctgtttcca gtattaattt ttagctgata
     1021 atttgactgt gaccattttt tagactaacc ttactttaat aaattgaaca aaaattgatc
     1081 aaaatgtgaa tttcatagtt taatgacaag gaggtcatat gaaaattcca tttgaaggga
     1141 aatggcgtgc gaccattacc aaacgtgagg ctggttttag ccaacgtgtc gtggttggag
     1201 gcgcaaccag cgggaatggc agttacaatg gtgttgttgg caacagcttt gttttcgaag
     1261 acggttgggt tgaactccag tggaataaca acgcaggttc aggctggcag gaaagcgctg
     1321 tcattagttc tgtgggaatg acgagtccgc tagttgttgt aaaatttgtt tcagccgatg
     1381 acaattttcc aaatcaacga gacggcgatt atgatgattt gcaagtccgt tttgaacatc
     1441 ttgatgctcc ttttgaagta acgcaaagac cttacgctct tgatcgaggc tcccttgtca
     1501 tgtttcctga cggcattttc gatgcgagcc aggggattca atacatggga gtacaaatta
     1561 aaaacacctg gttttttgat tggcaaagta attttccggc aacagggatg aaaattggta
     1621 ttgcgccagt gagtcgttcc gccttgtctg ctgctggtat tgtggtgatt gatagttgga
     1681 cgccacagga acaagaaggt ttggggcaaa tcatggatgg tggctttgtg agagttcctg
     1741 atctaaaaat taatgaggaa acaacaattt acttcaagct ggatgtcagt aatgccaatc
     1801 catccaaacc tgaaatcggt tttgtggcac agcgcgatgc ttatgatcca cactatgatg
     1861 aaccaacacg aatagttgat aaaaaaattt tcatcagtcg ctccagttac aatcctgcaa
     1921 ccagggaatt ggtgactgcg atccctgaag gtactttgta cctgaaactt aataaaattg
     1981 ccattgatcg caaaggcgct acaaaagcag ctcaggaatt gaagaaatgc cttaagcagc
     2041 atggaggtgg cataaaaggg atgtcaaaat ggctgcataa gctaagccgt ggtttttggc
     2101 aaaatagtaa agacaaggaa cgttggtgca aggagcaagg caatgatgca ttacgtaagc
     2161 ttttaaaaca gcttttagat ggaaaggaca ttgatatttg tcatcttaag gctattttgg
     2221 atcaatgttg tgaacgccca ggcggagatt gcgagtgttg tccttgtggt gatggagatg
     2281 gcaggccggg tggttgggat gatggtaccg gggttgatgg ctggtgccga gtgaaacctg
     2341 tattctggtt accgctggat tttgaatata aggtagtccc taacccggct tacattggcc
     2401 agtttggtcc tcttgctttc gaggatccct ggtggaaagt agttttaatc atcttggcga
     2461 ttatactggc tatcgcttca gtcatttatg attatgttta tgcttcagaa gatgaccaat
     2521 acattattgg gcaggttgtc cgtaatgggg atgctccaat caatctggtt gatgcagcag
     2581 tgagtaatct taatggctct cgtggagtgg atctgggaca actggatgcc cagggtaatg
     2641 atattaacaa cggcagcccc attactgcat tagacagtat tattcagctc aatcgtacag
     2701 cagccggaag ttattttggt acgcttgacg ctgttttggg taatgtcgtc tggaagtcag
     2761 gggctaccag tgcaacaacg agaggattta tcagtactct gggaactttc gtcagcatac
     2821 cttatgacga gcatgatacg ctttcaggta ccgttactta cactaacgaa gtggttatca
     2881 gtgaacttgc tgcgattccg caaacactct cagatcctgg tgactcggga tccgtatggg
     2941 ttgatctggc aacagcgcgt ccagttgctt taaattacgg cagcccaaca gactccagtg
     3001 ataatcgggc ttttgctaat ccgatacgct ctgttattga cgtgcttaac ttacggtttt
     3061 aatgaaaagg aagtaagaaa atgaaaaggg aattttttca aaaactcgac aagattcttg
     3121 atattgcttc cgaacataag caaatgttgt tgcaatatcc tgatgtagtc atggtaggtg
     3181 ttggacctga gcgtcgcaag ggaaaaatta caggggagcc tgcgatcatt gttacggtac
     3241 gtaaaaagcg ctcatccagt gaattaaaga aacgggggga aaagcccttg cctcagacaa
     3301 ttaagggaat tcctgttgat gtgattgagc ttggcaaagc cgtggaagct cccgaaatta
     3361 tcgctgttca ggaaaaagca aaaaaaacaa ttagtaaaat tggggcaaaa tggttacaaa
     3421 caccaaatat tacagcaata gggtatggct acaaagagaa aggaggggaa atcgatttta
     3481 acaccatagc catccagttt tttgtcaaaa agaaatttcc tatggctgag ctgcaaaagc
     3541 agcaaataag ccccattcct gaaaaaatta atggggtgag tactgacgtt attcagttat
     3601 ctcccatccg cccggctgcg gcttccggtt ccagaggaga tcgtcatgat ccgctcaaag
     3661 gaggtgttgc gattggagta ggaagcaagc catttcatta cggtacttat ggtgccacag
     3721 tatttgatag aaccactggc gaacaactct ttcttagcaa tcaacatgtt cttgatgcct
     3781 ctcctggaac agacaccata cagccttcgc cagtcttatt ggatgattca gtggaagtag
     3841 ggtttcaatt ggatgtttgc ttacctatcc attttatcag gctggatact ccaaatacta
     3901 ctgttggcac tgtgttggct ggggctgccg tagctgcagc tgttgccgct gctttaagtg
     3961 atgaaattga tccgacaagg cgaggtcagg aagcaacaat gcctgcgaca ggatctaaaa
     4021 cattatttga aacccaaaaa gttaaaatgg attatcctga attgcccatt ccagggacac
     4081 cgtttaaagt aaagacagct tgggattatg aacgccacac cgatgcgggt attaagatgt
     4141 tcagtgtcag tgaagaaaaa aagaatcccc atgttttacg cgataaatta ttaattacag
     4201 acaaaaagct ttatcaccct ggggatacta ttcgtatttt tggtttaatc ttgccggagg
     4261 aatgtgctcc tgcatcacag tccaggaacg atcgtcaacc cattaattcc cgggaattgg
     4321 aagatataac ccattatcct aatgtggctt caagtatcaa tcttgatctg gtagcctcaa
     4381 tggattctga cattgcctct tcgccagcgc gtcgcacgaa tacaagggct gctgtcatta
     4441 accccaaacg gtgccgttgt gatcgttatc acccggttgc cattcttaca ccaacgacgg
     4501 aggatcgtgc ttttccagtt gttttgcgtg agccagtctt tgcgcaaaaa aaccagattt
     4561 tccaagattt gctaaccgta gtgaagcaac gagatgatga aaagttgctg gagcgagtgt
     4621 ttgttctggt tcgttatggt tgtatatata ccggcacatt acgtgtaaat aacattccag
     4681 ctggtccatg gaaacatttt ttctatgttc aaacagttaa catcgcaaca agtgatatga
     4741 agccgctgct ggcagcgcaa attattggcg gcttacctgt ttcccaaaat gctaaagcgg
     4801 acctggatat agcctgtgga ccactcactt gggaagatgg tgagtttgat attgagttga
     4861 tatagagtgt actgcagggc tgattcatat tggctaatag ccatggaata atgtgagttg
     4921 tattgataag taatacctta actctgtacg gtacgatttt tgggaccgtc gttgcgagga
     4981 gcgtagcgac gaagcaatcc agtggcctga attcgttgaa gtagctcact gattcattga
     5041 taacccatat tttttccgct aagaaacgac atggagtcgt gaatgagtat cactgagctg
     5101 gcagatattt taaatggtta tttttcatgg aacaagtcgc gaatcgagtg ttttgcgacg
     5161 atgttaatct ctttaatcaa agtaagaacg gtcaatttaa ctgagattgc atgtggtttt
     5221 tctagccctg ctaaacaaga ttcaaggtac acaagaatca aacgattttg aagacacacg
     5281 catgactcaa ccggatcgca ttgaaaaact actggtgcta ttgaccattg ctttttgttg
     5341 ggcccataaa actggagagt ggcggcatgt tcagaagaca attaaaatca aaaaacatgg
     5401 tcgcaaaggg gtaagttttt ttcgttatgg actt
//
LOCUS       DAXLDT010000192         5334 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXLDT010000192 DAXLDT010000000
VERSION     DAXLDT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.192"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..905
                     /locus_tag="ACF2SP_002755"
     CDS             <1..905
                     /locus_tag="ACF2SP_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg1803 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360523.1"
                     /translation="CDLIDIQIKKYVELAKLKVGEDAIINAALIYEEVNELLGFAEKL
                     LKEIEANSKSDSMDMTIFDHVFEQVKFYVEQEYLRSYAEWLLIENNSKDTVYNRTKEQ
                     LLKLSEIKSFDDKGFSQPSTEIQQQCQHDSVQIVYSILNLAREVRKNPDMGFDDSIPH
                     NKLNVLRRNATTRYVEFGNEIDKLHQHSEEFLQQSSLGVFDAELAYESALSKAKDHDL
                     KLNTLFGRYKENKETASVPQSLSLVNKVDEENIMTEKKPSTEVSDNKTPFFSTSNLLF
                     FGGIVLAAVIFVPRLLTHLKVNKL"
     gene            1208..2254
                     /gene="recA"
                     /locus_tag="ACF2SP_002756"
     CDS             1208..2254
                     /gene="recA"
                     /locus_tag="ACF2SP_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFD2360524.1"
                     /translation="MEENKQKALSAALSQIERQFGKGSVMRMGDSTVSRDIEAISTGS
                     LGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAECQKMGGTAAFIDAEHALDPS
                     YAQKLGVKVDELLVSQPDTGEQALEITDMLVRSAAVDVVIIDSVAALTPKAEIEGEMG
                     DSHVGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRIGSIKKGEEILGSETRVKVVKNKVAPPFKMTEFDILYNEGISRESEIIN
                     LGVQLNLIEKSGAWYSYKQEKIGQGKENVRLYLKENPQVAAELEQQIRTELLEKKLSV
                     LASSSEDLFETIDD"
     gene            2247..2699
                     /gene="recX"
                     /locus_tag="ACF2SP_002757"
     CDS             2247..2699
                     /gene="recX"
                     /locus_tag="ACF2SP_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFD2360525.1"
                     /translation="MTKAFDSALRLLTRREYSAMELCDKLKQKGFSTNDVQNALYECQ
                     RLGYQSDVRFVENYIRVRIHQGYGPLKIRQELKNKGIDPDLIQSVLHQEKDNWVNYAL
                     RAWEKKFKRQDDFSYSEIQKQQRFLLYRGFDRDVISKVFKEVKSSYLI"
     gene            2717..5299
                     /gene="alaS"
                     /locus_tag="ACF2SP_002758"
     CDS             2717..5299
                     /gene="alaS"
                     /locus_tag="ACF2SP_002758"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFD2360526.1"
                     /translation="MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQ
                     FKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
                     SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHL
                     LPKPSVDTGMGLERLAAVIQGVHSNYEIDSFQYLIKAIAQLGQNIDLNHTSLKVIADH
                     IRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFFFKLVQPLIDVMGDA
                     YPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLKGQELSGEVAFKLYDTYG
                     FPIDLTADIIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGY
                     EKESMEAKIIGLLQEGNEVKSINKGAKGAVILDHTPFYAESGGQVGDKGLLIGKNCSF
                     QVDDTQKVGQAVVHYGEVIKGELTLDLSIHAQVDHIRRDAIRLNHTATHLLHAALKKI
                     VGQHVQQRGSLVDAERARFDFSHFEALNPQQIQQIEEVVNAQIRANNEVITQVMDIES
                     AKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIR
                     RIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEKISQLLLDNKNQEKMIAKLLS
                     EKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQ
                     NKMNVIAGVSKNIIGKTPSAAQLVRHLCGKGGGRDDMAQGGGNVPEDLNSKIKEIREM
                     IEKI"
BASE COUNT         1733 a          858 c         1172 g         1571 t
ORIGIN      
        1 tatgtgatct gattgatatc cagattaaaa aatatgttga gctcgcaaaa cttaaagtag
       61 gtgaagatgc gataataaac gccgctctca tatacgaaga agtgaatgaa ttattaggat
      121 tcgcggagaa gttattaaag gaaatagaag ccaattcaaa atctgactca atggatatga
      181 ccatttttga tcatgttttt gagcaagtaa aattttatgt ggagcaagaa tatctgagat
      241 cctatgcaga atggctgctt attgaaaata actctaaaga cacagtctac aatagaacaa
      301 aagagcaatt actcaagctt tcagaaatta agagttttga tgataaaggt ttctcacaac
      361 caagcactga aattcaacag caatgtcaac atgatagtgt tcagatagtt tattccattt
      421 taaatttggc aagagaggta agaaaaaatc cggatatggg gtttgatgat agcatcccac
      481 ataacaagct caatgtatta agaagaaatg caactaccag atatgttgaa ttcggaaatg
      541 aaatagacaa attgcatcag catagtgaag aatttttgca acagtcgagt cttggagttt
      601 tcgacgccga attagcctat gaatcggcac tatcaaaggc aaaggaccat gatcttaaat
      661 taaatacttt gtttggtcgt tataaagaaa ataaggaaac cgcttctgtt cctcaatctc
      721 tctctttggt caataaggta gatgaggaaa atattatgac agaaaaaaaa ccttcaactg
      781 aagtgtctga taataagacc cctttctttt caacaagcaa tttattattt ttcggtggta
      841 tagttttagc tgctgttatt tttgttccca ggttgcttac tcatctcaag gtcaataaat
      901 tatgacacag cattatgagt agttaacatt ttcgttattt tgattgtaaa accttggttt
      961 ttatgtcgga ttgtctatat atttttatat tgccccgtta tcaagctaaa aatcaaggtt
     1021 tgaagttcaa actgacaaca ccttcacttt tgaacaaatg gttattccac gtagcttata
     1081 ataaaagact aatcatcagc caccgtatgc tacttttatt tagtaataca ctaagattac
     1141 tgtttgctgc tcgctcacat ctgtgtgata attttgttct tgcacacatt ataatctgga
     1201 ataaattatg gaagagaata aacaaaaagc actatcagcg gcgctttcgc aaattgaacg
     1261 ccaatttggt aaagggtctg taatgcgtat gggagatagc acggtatcac gggatattga
     1321 agcaatctct acaggttcat taggtcttga tattgcctta gggataggcg gtttgccaaa
     1381 aggccgtatt gttgaaattt atggccctga gtcttctggt aaaaccacgc tcactcttca
     1441 agtgatcgct gaatgtcaaa aaatgggtgg tacagcagca tttatcgatg cggaacatgc
     1501 tctggatcca agctatgctc agaaacttgg tgtgaaggtg gatgagcttt tggtttctca
     1561 gccggataca ggcgagcaag ctctggaaat tactgatatg ctggtgcgtt ctgcagcagt
     1621 tgatgtcgta ataattgact cggttgctgc cttgactccc aaagcagaaa ttgagggaga
     1681 aatgggcgat tcccatgttg gtttacaggc aagattgatg tcgcaagccc tgcgtaaatt
     1741 gaccgctaac attaaacgct cgaacacact ggttattttt attaatcaaa tacgtatgaa
     1801 aattggcgtg atgtttggta gccctgaaac cactacaggg ggtaatgctt tgaaatttta
     1861 tgcttcggtt cgtttggata ttcgccgtat tggttcaatt aaaaagggcg aagaaatatt
     1921 aggcagtgaa acccgagtta aagtagttaa aaataaggtg gcgccaccgt ttaaaatgac
     1981 tgagtttgat attttatata acgagggtat atctcgtgag agtgaaatta ttaatctggg
     2041 tgttcaattg aatttaattg aaaaatcagg tgcctggtat agctacaaac aggaaaaaat
     2101 cgggcagggc aaagaaaatg tcagattata tttgaaagaa aatccccaag tcgctgcaga
     2161 gcttgaacaa caaatccgta ctgagctctt ggaaaaaaag ttgtctgtgt tggctagttc
     2221 atcagaagat ctttttgaga ctatagatga ctaaagcatt cgatagcgcc ttgcgtctgt
     2281 taaccaggcg cgagtatagc gctatggagt tatgtgataa actgaagcag aaaggattta
     2341 gcaccaatga tgttcaaaat gctttgtatg aatgccaacg tcttgggtat caaagcgatg
     2401 tgcgttttgt agaaaattat atcagagtac gaattcatca aggctatggc cctttaaaga
     2461 tcaggcagga attaaaaaac aagggaatag atccagatct cattcaaagt gtgctgcatc
     2521 aagaaaaaga caattgggtc aattatgctt tgcgggcatg ggagaaaaaa tttaagagac
     2581 aggatgattt ctcttatagt gaaatacaaa aacagcaacg gtttttgctg tatcgagggt
     2641 ttgataggga tgttatttca aaggtattta aagaggttaa atcttcttac ttaatttaac
     2701 tggtaaatat taaaaaatga aaagttctga aattagacaa gcattcctta actattttgt
     2761 tcaacgcggt catcaaatag tagcttctag ctcattggtc ccttcaaatg atcccacttt
     2821 actatttacc aatgcaggta tggtgcaatt caaggatctt tttttaggtc ttgaaactcg
     2881 ttcatatcag cgtgctgcta cagcacagcg ctgtgtgcgt gcaggtggta aacataatga
     2941 tctggaaaat gttggttata cggcgagaca tcatacattt tttgaaatgt tgggtaattt
     3001 tagttttggt gattatttta aacgcgaagc tattcaatat gcttgggaat tcctaacaga
     3061 agttttacac ataccggcag aacggctatg ggttactgtc tataaagagg atttggaagc
     3121 agaagacata tggctaaagg aaatgaaggt atctcccgag cgattctctc gatgtgggga
     3181 aaaagataat ttctggtcaa tgggggatac gggtccatgt ggtccttgca cagaaatttt
     3241 ttatgatcat ggtcctgaag tagcaggcgg tcccccgggc agtccagatg aagatggaga
     3301 tcgttatata gaaatatgga atctggtatt catgcagttt aacagggaca gagagggaca
     3361 tttacacctc ttgccaaagc cttctgtgga taccggtatg ggattggaac gattagccgc
     3421 ggttatccaa ggtgtccata gtaattatga aattgatagt tttcagtatt tgattaaagc
     3481 aatagcacaa ttgggccaga atattgattt aaatcatact tccttaaaag tgatcgcaga
     3541 tcatattcgt tcctgctctt ttttgatagt tgatggagtt ttgcctagta atgaaggcag
     3601 aggatatgta ttaagaagga ttatccgcag agcagtcagg catgggaata aattaggttt
     3661 acccagtcca ttttttttca aacttgttca gcctttgatt gatgtcatgg gagatgctta
     3721 tcccgagcta atcaattcga aagcgcatat cgaaaggatc ttgcaacaag aagaaaatca
     3781 gtttacccgt actttggaac aaggattgcg tttattacaa gatcatatta aaaatttgaa
     3841 aggccaggaa ctatcaggag aagtggcatt caagttgtat gatacttatg gtttccctat
     3901 cgatttgaca gcagacatca ttcgtgaaca aggtttacat attgatatgg aagcatttaa
     3961 tcaattgatg caacagcaaa gagaacagtc tcaagccgcc agtcagttta ctacagatta
     4021 tcatgccgtg tctcaattgg atcatcagtc tgaatttcat gggtatgaaa aagaatccat
     4081 ggaagcaaag attattggat tacttcaaga aggtaatgaa gtgaaatcta ttaataaggg
     4141 agcaaaaggg gctgtaattc ttgaccatac cccattttat gctgaaagtg gtggtcaggt
     4201 aggagataaa ggattactca taggaaaaaa ctgttctttt caagttgatg atacacaaaa
     4261 agtagggcaa gccgtcgtac attatggaga agtaattaaa ggtgagctca ctttagattt
     4321 atcaatccat gcgcaggtag atcacataag acgggatgca attcgactga atcataccgc
     4381 aactcatttg ttacatgctg ctcttaaaaa aattgtagga caacatgttc aacagcgagg
     4441 atctctggtg gatgctgaac gggcacgttt tgacttttct catttcgagg cattgaatcc
     4501 tcaacaaatc cagcaaattg aagaggtagt taatgcacag atccgggcta ataatgaagt
     4561 aataactcaa gtaatggaca ttgaatcagc caagcaaagt ggtgctgttg ctttattcgg
     4621 ggaaaaatac tccgacgcag tacgcgtatt atccatgggc gatttttcca aggagctttg
     4681 tggaggaact catgcacgac gtactggtga tataggtctt ttcaaaatcg tagcagaata
     4741 tggtattgct agcggtatta ggcggattga gatggttacg ggtcgttatg cgttagcctg
     4801 ggtcaatgaa cagctgggat ttatgaataa tctggcagct acccttaaaa caacacctaa
     4861 tagtctacag gaaaaaattt ctcaattatt gcttgataat aaaaatcaag aaaaaatgat
     4921 tgcaaaactt ttaagtgaaa aagcacaaaa atctggagca gatattcttg gtgagatcga
     4981 ggagatcaag ggtattaatt tattgattaa gcagttagag ggtatggata gccaaaccat
     5041 gcgccatacc atggatcaat taaaatcaag aattgattca gcggtgatca tattatttac
     5101 tattgaacag aataaaatga atgtcattgc cggggtaagt aagaatatta ttggcaaaac
     5161 gccaagtgct gctcaattag taagacatct ttgtggtaaa ggaggtggcc gagatgatat
     5221 ggctcaaggt ggtgggaacg ttcctgaaga tttgaacagt aagatcaaag aaataaggga
     5281 aatgattgaa aaaatttaat atttccagat ttttcttttc gactgcagaa aagc
//
LOCUS       DAXLDT010000193         5289 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXLDT010000193 DAXLDT010000000
VERSION     DAXLDT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.193"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..888
                     /locus_tag="ACF2SP_002759"
     CDS             <1..888
                     /locus_tag="ACF2SP_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFD2360527.1"
                     /translation="GFADRKSKKPVTKNTIFELASITKIFISTLVALEVQKGKLNVTD
                     YAVQYTPELSKTNGLPIDRVQIVHLATHTASFPKQMEQFGVNKGNIQAFFARLKTWRP
                     PVKIGTHYKYSNIGFGYLGYVLENASNETLPNLLKSNITKPLGMSHTFFNVPESLANY
                     EAQGYRPKNKPAPYYTPANFLGGGALRSSAADMLVFLKANLGIQPGTASPELLSAMQY
                     AQQPFFEVSPNFVMGLGWQRVKRGRHIVITKNGANQGFSTFIGFSPAKKLGVVVLINQ
                     ARGKATALGNQILNSLLGL"
     gene            1139..1576
                     /locus_tag="ACF2SP_002760"
     CDS             1139..1576
                     /locus_tag="ACF2SP_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360528.1"
                     /translation="MKKSNHVHSLLLLTAITFSPVIKASPVGLIVDIYCPTTQGGPNV
                     ITNFGDYIAGYGMENILSQNNPIYFKSISLAHDVPAQLGNYYNETTSYNSTTGQVTCS
                     YISNNPTEPRFAVAYTLTNGRGGSVQWQSGNSINIIFPVGLKS"
     gene            complement(1789..2082)
                     /locus_tag="ACF2SP_002761"
     CDS             complement(1789..2082)
                     /locus_tag="ACF2SP_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360529.1"
                     /translation="MNVNSENAQPGEAVTAEDYSRAMNFIGQNLLSSLTQSVEKLPPP
                     FRNRRLVCQALSAFLTNVIYKQFPEQPELCQQMLDDITRHVSMQLDKIPQPSK"
     gene            complement(2308..2403)
                     /locus_tag="ACF2SP_002762"
                     /pseudo
     CDS             complement(2308..2403)
                     /locus_tag="ACF2SP_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
     gene            2428..2700
                     /locus_tag="ACF2SP_002763"
     CDS             2428..2700
                     /locus_tag="ACF2SP_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360530.1"
                     /translation="MSRLKEMRDAFKQNNPEDIFDLFTKSEYLQGFYMLCNLIRRNDE
                     TLFDDMRFLLSIPNLKVLASIGVNPEEENELIRMAQRLVVGLTCVP"
     gene            2922..>5289
                     /locus_tag="ACF2SP_002764"
     CDS             2922..>5289
                     /locus_tag="ACF2SP_002764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360531.1"
                     /translation="MLSTSNPLLHSAILNLDVENCKDLLKDNKISDINEFAELIRDIL
                     ITPKINEYRSSFDRAVQILDVFLDQGLSLDVYKLPEDIFHRLLMEYPETTYKVIDDFQ
                     KKINKDSFPNYVEFLKKNLHAMLYSYSVLGFRDENGPAFKKNFDLIFKFLGQNQVDYT
                     KAPEFEFMQGYDSSSFWHLMQTLVQNDCVMSFQSLREWPVVSQSIELLESKAIDEDSP
                     AIFSKIWLANAKNMSLDETFSKIGSLYNKQAHKCIAVLMENLDLNDEVIKRIRNLRTV
                     TSKDMFNAYMSGDFRKLKNRDNGKVILALFKNNYRLAAIEVFDPARFNSADEKEVKER
                     IRDLFLNLFASTPFDKINKLVTALPIDHPHLQIINEVYLKVKTEYCQAAPCFSRYVKP
                     QHIDKLKGFYGNKAGKINDDIQFGAKRMELVYELNKLLSNSTHYVTPSKPPFNSLLPS
                     SSKTQLAAFALEKLSEFLLQTGRSKEVSRLRESEDFGTPVKAQYSFALPLIRNVFYFS
                     DDVKLNKHDYSVSYGLFYNDLQVNNIMETKQIWSGPIDETHAAPNIVWTHGLAPLNAT
                     WPNIENLFAEIWSMDLSFAGKYSDYRDKLTVFYEKLAELSWLIGNTQPLERGTGSFAE
                     TVVALMHVHHGLNPPILKNDFPQLDVLNITFPLETYKNIFPYFFEPSTVPKHLRSFTN
                     IPEMEKLSISQQIQHLFTQFQRQQPNNPGYELDELSIANILKNHATKNKPPRFSFLNP
                     EKDLLAKTSNYYFSLFNKADNPEQKILLVLTILQFSKNKNLQLDIAKQI"
BASE COUNT         1767 a          975 c          918 g         1629 t
ORIGIN      
        1 ggatttgctg acaggaaaag taagaagcca gtcactaaaa atactatttt tgaattggca
       61 tcgattacca aaatattcat tagcactctt gtagccctgg aagttcagaa aggaaaattg
      121 aacgtaacag attatgccgt tcaatatacg cctgaacttt caaaaaccaa tggactacca
      181 attgacaggg ttcaaattgt gcatctggcg actcacacag caagctttcc caaacaaatg
      241 gagcaatttg gtgtcaataa aggcaatata caggctttct ttgcccgtct taaaacatgg
      301 cggcctccag taaaaatagg aactcattat aaatattcca atattggttt tgggtatttg
      361 ggatacgtat tggaaaacgc ctcaaacgag actttaccta atctgttaaa atcgaatatt
      421 accaagccat tgggaatgtc ccatactttt ttcaatgtac cagaaagtct ggctaactat
      481 gaagctcaag gttatcgacc taaaaacaag ccagcacctt attatacacc tgccaacttt
      541 ctaggtggag gagctttacg ctcttctgca gctgacatgt tggtattttt aaaagcaaat
      601 ttaggaattc aacctggcac cgcatcacca gaattacttt ctgcgatgca gtatgcccaa
      661 caaccatttt ttgaagtcag cccaaatttt gttatgggat taggttggca aagagtgaaa
      721 agagggaggc acattgtgat cactaaaaat ggagcaaatc agggatttag cacctttatt
      781 ggtttttctc ctgcgaaaaa acttggagta gtcgtattaa tcaaccaagc aagaggtaag
      841 gcaactgcat taggcaacca aatacttaat agccttcttg gtttataatt catacattgg
      901 cacaaaagtt aatactgtct ttcataagca agttatacaa cacttaaagg ctatttgttt
      961 gttaagaata gattcacggg cattgacaat caaaatgtcg tgcaacatga ttacctttgt
     1021 accttttctc tgtatggctt ggcttacaac gtggatatga tgattttaat ctgaagcaat
     1081 ggctaacaga acaacaggtc aaaccagcaa cttaaaataa ccgaaaaagg gagtttcgat
     1141 gaaaaaatcc aatcatgtac actcattgtt attgcttacc gctattactt tttcacctgt
     1201 tatcaaggca agccctgtag gtctgattgt agatatctat tgccctacta cccagggagg
     1261 accaaatgtc atcactaatt ttggagatta cattgctggt tatgggatgg aaaatatttt
     1321 atcacaaaac aatcccattt attttaaatc catttcacta gcccacgatg ttccagccca
     1381 acttggaaat tattacaatg aaacgaccag ctacaacagc acaacaggcc aagtcacttg
     1441 cagttacata agtaataacc caacagaacc tcgctttgct gtggcatata ccttaaccaa
     1501 tggcaggggc ggttctgtcc aatggcagtc agggaattca atcaatatca tctttccagt
     1561 tggtctaaaa agttaaccaa agtttactgg attaaaatgc ctgactgtct tcttcgtctc
     1621 ccagcaactc tttctggaat tgatttgctg ggaagattga cagaaactct gcaatcaaag
     1681 ccaaaccaag cagaagaaca tatctgataa ttggggtatt caagctgaaa cgcatgattt
     1741 atttgatttc aactcaggcc aaattagctg atacagatta ctaacgtttt actttgatgg
     1801 ttgagggatt ttatccagtt gcatactgac atgcctggta atatcatcga gcatttgttg
     1861 gcataactca ggttgctctg gaaactgttt ataaataaca ttagtcaaaa atgcagacaa
     1921 agcctgacaa accaacctgc gattacgaaa cggtggcggt aacttttcca cagattgagt
     1981 taatgatgac aataaatttt gacctataaa attcattgcc ctggaataat cttcagcggt
     2041 tacagcttcg cctggttgag cgttttcact attcacattc attttttatc ctgtcagtgt
     2101 gtgatcaagc cggattatag agtctatctc gagctacttc aacgactgtt tcagctcttt
     2161 ccataaaacc agtatgatta tccattaaaa caacaaaata cctcattaac aggacttacg
     2221 aaaaacccca agattgagaa aaaaagtatt tttaataaag agattagggg gaacgtaagt
     2281 cctgattaaa actcatttat gatgtagtta tggttttaca atgacaacgg gctctggcgt
     2341 gccgcccaaa agactaaaga ccaaaagcac aataaacatt acgataaaga ggaaaagaag
     2401 tacgtcaatc catttattgt gaaacacgtg agccggttaa aagaaatgcg tgatgccttt
     2461 aagcaaaata acccggaaga tatctttgat ttattcacaa agtcggaata cctgcaaggc
     2521 ttttatatgt tatgtaactt aatccgtcgc aatgatgaaa cattatttga tgatatgcgt
     2581 tttttgttga gtattccaaa cttaaaagtc ctggcatcta ttggagttaa tccagaagag
     2641 gagaatgagt taattcgcat ggctcagagg ctggtggtcg gcttgacttg tgtgccgtag
     2701 cttaagtcag ttaagaacaa tcaaaggagt tgatgcttgc gacaataaaa ttgtcatatc
     2761 actcaaacgt tatggctatg tttataaacg tttacctatg cattactcat cggatataac
     2821 tttaaaacca cttaaagtgg gttcaactta atacgattcc aaaaccattt tgttcaaatt
     2881 tttatactat attatattaa aaatgttctt ggaaaaaaat aatgttgagc acatccaatc
     2941 ctttgcttca ttctgctatt ctgaatcttg atgttgaaaa ctgtaaagat cttttaaaag
     3001 acaacaaaat tagcgatata aacgaattcg cagaattaat tagggatatt ctgattactc
     3061 ctaaaattaa tgaatatcgt agttccttcg acagggcagt tcagatttta gatgtttttt
     3121 tggaccaagg tttatctctc gatgtttaca aattacctga ggatattttt catcgtttgt
     3181 tgatggaata tcctgagaca acctataaag ttattgatga ctttcaaaaa aaaatcaata
     3241 aagattcatt ccctaactat gttgagtttt tgaaaaaaaa tcttcatgcg atgctatata
     3301 gctattctgt attaggtttc cgtgatgaaa atggccctgc ttttaaaaaa aatttcgatc
     3361 ttatttttaa gtttttagga caaaatcaag tcgattatac aaaagcacct gaatttgaat
     3421 ttatgcaagg gtatgatagc tcttcttttt ggcatttaat gcaaactcta gtgcaaaatg
     3481 attgcgtgat gtcttttcaa tcactccgcg aatggcctgt tgtctctcaa tcaatagaat
     3541 tattggaatc aaaagctata gatgaggaca gtcctgcaat attcagtaaa atttggttag
     3601 caaatgcaaa aaatatgagt ttggatgaaa ctttctctaa aattggttcg ctttataata
     3661 aacaagccca caaatgtatt gcggtcctta tggaaaactt agatctcaat gacgaggtaa
     3721 taaagcgtat taggaattta agaacagtta cttcaaaaga tatgttcaat gcttatatga
     3781 gtggggattt cagaaaactc aaaaatagag ataatggcaa agtgatactt gctctgttta
     3841 aaaacaatta tcgtcttgca gcaattgagg tgtttgatcc agctagattc aactcagctg
     3901 atgaaaaaga ggtaaaagaa agaattaggg atttattttt aaatttattt gcatccactc
     3961 ctttcgataa aattaataaa ctagttactg ctcttcctat agatcatcct catctacaaa
     4021 ttattaatga agtttatttg aaagttaaaa ctgaatattg tcaagcagca ccttgttttt
     4081 cacgatatgt aaaaccgcaa catatagata aacttaaagg gttttatgga aacaaagctg
     4141 gtaaaatcaa tgatgatatc caatttggcg ctaaacgaat ggaattggtt tatgagctca
     4201 ataagttatt atctaattca acacattatg tcaccccaag caaaccacct tttaattccc
     4261 ttttaccctc aagcagtaag acgcaattag ctgcttttgc tcttgaaaaa ttatctgaat
     4321 ttctgctaca gaccggacga agtaaggaag ttagtcgatt acgagagtct gaagatttcg
     4381 gcacgcctgt taaagctcag tattcatttg cattacctct tattagaaat gtgttttatt
     4441 tttccgatga cgtaaaacta aacaaacatg actattctgt cagttatggt ctgttttata
     4501 atgaccttca agtcaataat attatggaaa ctaaacagat ttggagtggg cctattgacg
     4561 agactcatgc tgcacctaac atagtatgga cacatggcct ggcaccatta aatgccacct
     4621 ggcctaatat tgaaaattta tttgcagaaa tctggtctat ggatttatcc tttgcgggaa
     4681 aatattctga ctatcgagac aagcttacgg tcttttatga gaaactagct gagttatcgt
     4741 ggttaattgg caatactcaa cccctagagc gtggcacagg gagctttgct gaaactgtag
     4801 tagccttgat gcatgttcat catggtttaa atcctcccat tcttaaaaat gattttccac
     4861 aactggacgt actgaatatt acatttcctc tagagaccta taagaatatt ttcccctatt
     4921 ttttcgaacc atcaactgtt cccaaacact taaggtcatt tactaatatc cctgagatgg
     4981 aaaaactctc tatctcgcaa caaatacagc atttattcac tcaatttcaa cgacaacaac
     5041 caaataatcc tggctatgaa ctcgatgagc tctcaattgc taacatacta aaaaaccatg
     5101 caacaaaaaa taaaccaccc agatttagct ttttgaatcc agaaaaagat ctcttagcca
     5161 aaacatcaaa ctattatttt tctctcttta ataaagcaga caatcccgaa caaaaaattc
     5221 ttcttgtttt gactatatta cagttttcta aaaataaaaa tttacaatta gatatagcaa
     5281 aacaaattt
//
LOCUS       DAXLDT010000194         5261 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXLDT010000194 DAXLDT010000000
VERSION     DAXLDT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.194"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1611
                     /gene="legLC8"
                     /locus_tag="ACF2SP_002765"
     CDS             <1..1611
                     /gene="legLC8"
                     /locus_tag="ACF2SP_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegLC8"
                     /protein_id="HFD2360532.1"
                     /translation="IKQHQVKLVLKTSQDIQLFLNALRDSRNHGISSLSELDLSDTRF
                     TNQELSDLVTALNNIPGIKSLRLDSCGLKDSDTVELSKLTHIKKLSLKSNYLKNRPMF
                     NSMLEALYLDYNTELSASYVLFSLSRNAAALKKLSLRNCGVTDANLEYLTRPESRLKS
                     LTHFNLRRNNITHQGVDSIAHLQSLTTIDLSQNTGIGDEGVSRLALLKQLRTLYLDNC
                     GIGAEGIKAIAKMNLQTVDLSFNPGLKKEWGLDDIRPNHTIRTLLLTFCSLNDNHAKL
                     IVSKFPAATDLNVANNNMTRAGVKTLLSNPIIENLDVSTQSLYAKSTGASGTGMISPR
                     IMMKKKEIKQQEKEKAQDLLDTICNTITLKSINLEHTGITSRMLLSLIPDETDHKRYL
                     KKINGVSCKELKPKLEQQIALRKAEKQSPPPVIIAVEDSKPSDLMIKSGSSSKTEAQT
                     REDGLIASPDEKIKALELENQKLKDELATARKRIFELENQSVSGSKTRKKTSIPPLAI
                     PVMFQDFAKPGKNETGDNTSNSITAIPK"
     gene            complement(1675..2640)
                     /locus_tag="ACF2SP_002766"
     CDS             complement(1675..2640)
                     /locus_tag="ACF2SP_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2360533.1"
                     /translation="MDLSKRIEPKTWSFVEKIQNAGGKPIYDIPVAEGRAIFDQLQAL
                     PNEKPDVDVEDKTLPVGPGGKVDIRIVRPKGAKESLPVIMFFHGAGWVFGDYQTHGRL
                     VREIAVGTHAAVVFVKYTLAPEAQYPTQIEESYAAMKYIAEHGKQFNLDTSRFVAAGD
                     SVGGNMTSVMALLAKERGGPKIDYQVLIYPVTDANFDNASYKEFAEGPWLTKKAMEWF
                     WDKYLPNKEKRKEITASPLQASLEQLKGLPPALVITGECDVLRDEGEAYAHKLNAAGV
                     TVTAVRHLGTIHDFLMLNDLSETPACRNAVETIIGHLTHIFGKKK"
     gene            2869..4200
                     /locus_tag="ACF2SP_002767"
     CDS             2869..4200
                     /locus_tag="ACF2SP_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1888 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HFD2360534.1"
                     /translation="MLQFFEKGLQYSGENISADSYLMRKIIEAATPSADKGTVSAEKM
                     LGLVNPNQIEIVSGSENTYRLIVHKIKEAKKQILIQAFVWEPETQVVKDIRNALCDIK
                     HEVEVFLLVDQLDRLAQVFYHGEILPKKPKHDPACLGLDNLPQNIKLHIGTHVHNSIA
                     SNHNKAIWIDGDVILTGANFQQENYGPNGFHDAAMFIPGGAAEAVFYDFKAMWDKRTN
                     KSDELEISPIFTGQESTESYRNTNSCSVLYVTNTIRQKWALLPFYRAPLPEDPLNNAY
                     MAAIKYAKHTIRIAVPNLNSPEIIHALADFINNRNGHVYLLMGKGFNDFRERFYGGTN
                     QSSIDYLSSLLDEDRQNNLQVRWFHRVNDNEISIKKEVIHMKFMSIDGQVVIYGSSNL
                     DLLSLHNSHETNIVIDNADFAQRAIEQLYLPIFETSIPVLISPKRTCFVCS"
     gene            complement(4382..4735)
                     /locus_tag="ACF2SP_002768"
     CDS             complement(4382..4735)
                     /locus_tag="ACF2SP_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360535.1"
                     /translation="MKKVILYALPSLMLAILFSPAYADETPAQLTGTPAEQIQQLNAQ
                     IQSQLQEIQTKQQQQLDALNSQLQNQIKQVQSQLQEQIQTVNSQTQDQIKQIQATLQE
                     QIKQVQQQALQKANQ"
     gene            4892..5167
                     /locus_tag="ACF2SP_002769"
     CDS             4892..5167
                     /locus_tag="ACF2SP_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFD2360536.1"
                     /translation="MTKELCMRCYISGRVQGVWFRASAKKLAEQLMISGWARNLADGR
                     VEVFACGKEDKLEEFYTWLQKGPLNARVDVCTRENLPWKDYISFDVL"
BASE COUNT         1685 a          879 c         1038 g         1659 t
ORIGIN      
        1 ataaaacagc atcaggtaaa acttgtctta aaaacatctc aggatattca attattttta
       61 aatgctttgc gagattcgag aaatcacgga atctcgtcct tatctgaact tgatctaagt
      121 gatactcgct ttactaatca agagttgagt gatttagtca ctgctcttaa taatatccct
      181 ggaattaaat ctttacgctt ggattcttgt ggtcttaagg atagtgacac agtggagctt
      241 tccaaattaa ctcacatcaa aaaattatca ttgaaaagta attacttaaa gaaccgccct
      301 atgtttaatt ctatgcttga agcattgtat ttagattata atacagaact tagcgcaagt
      361 tatgtgttat ttagtttaag tcgcaatgct gccgctttaa aaaaattatc cttgcgtaat
      421 tgtggtgtta ctgatgctaa tttagagtat ttgactcggc ctgagtccag gttaaaaagt
      481 ctgacacact ttaatttgcg aagaaataat atcactcacc aaggagtgga tagcattgct
      541 catcttcaat cgttgactac tattgattta agtcaaaata ctggaattgg tgatgagggt
      601 gtatccaggc ttgcgctttt aaagcagtta agaaccctgt atttggataa ttgtggaata
      661 ggggcagagg gaattaaggc aattgccaaa atgaatttgc agactgttga tttgagtttc
      721 aatcccgggt taaaaaaaga atggggattg gatgacattc gacctaatca taccattcga
      781 acattattgt taacattttg cagcttaaat gataatcatg ccaagcttat tgtttcgaag
      841 tttccagcgg ctaccgattt aaatgttgcg aataataata tgaccagggc aggggtaaag
      901 actttattga gtaacccgat tatagaaaat ttggatgtaa gtacccaatc gctttatgca
      961 aaatcaacag gagcttctgg cactggcatg atctctcccc gaataatgat gaagaaaaag
     1021 gaaataaaac aacaggaaaa agaaaaagcg caggatttgc tagatactat ttgtaataca
     1081 atcacattaa agagtattaa tctggaacat acaggcataa cctccaggat gttattgagt
     1141 ttaattccag atgaaacgga tcacaaacgg tatcttaaga aaattaatgg tgtttcctgt
     1201 aaagagctta agccaaaatt agaacaacaa attgccttga gaaaagctga aaaacaaagt
     1261 cctcctcctg taatcattgc agtggaagat tctaaaccaa gtgatttaat gattaaatct
     1321 ggcagtagtt ccaagactga ggcgcaaacc agggaagatg gcttgatagc cagtcctgat
     1381 gaaaaaataa aagctctgga actagagaat caaaaattaa aggatgaatt ggcaacagct
     1441 cgtaaaagaa tttttgaatt ggaaaatcag tctgtatctg gatccaaaac aagaaaaaaa
     1501 acctcaatac caccattggc aatcccggtg atgtttcagg attttgcaaa accaggaaaa
     1561 aatgaaacag gtgacaacac cagcaattca ataacggcaa tccccaaata gcacaattat
     1621 tgtaaatgca ttcagcatga ctaacatcca cttggatgtt agtcatgtat tgttttactt
     1681 tttcttgcca aaaatgtgag ttaagtgacc aataatggtt tcaacggcat ttcggcatgc
     1741 cggtgtttca gagagatcat tgagcatcag gaaatcatgg attgtgccca gatgacgaac
     1801 tgcagtcact gtcacacccg ctgcgttgag tttatgggca taggcttcgc cctcatcacg
     1861 caaaacatca cactctccag tgatgaccaa tgctggtggt aggcctttaa gttgttcaag
     1921 agaggcttgc agcggacttg ctgtaatttc ctttcttttt tctttattgg gtaaatactt
     1981 atcccaaaac cactccatcg cctttttggt tagccaggga ccttcagcaa attctttata
     2041 ggaagcgtta tcaaaattgg catcagttac tggataaatg agtacttgat aatcaatttt
     2101 ggggccacca cgttctttag caagaagggc catgacgctg gtcatattac caccaacact
     2161 gtcacctgca gcgacaaatc gtgaagtatc cagattaaat tgcttgccat gttctgctat
     2221 atatttcatg gcagcgtaag attcttctat ttgtgtcggg tactgagcct ccggagctaa
     2281 agtgtattta acaaacacga ctgcggcatg agtacccact gctatttctc gtactagacg
     2341 accatgagtt tgatagtcgc caaaaaccca gccagcacca tgaaaaaaca taatgacagg
     2401 taaggattct tttgcacctt tagggcgaac aatgcgaatg tcaaccttac cgccaggccc
     2461 tactggcaaa gttttatcct caacatctac atcaggcttc tcattaggca aagcttgtag
     2521 ttgatcaaat attgccctac cttctgcaac cggtatgtca taaatcggtt ttccacccgc
     2581 attttgtatt ttttcaacaa atgaccaggt ttttggttct attcttttgc ttaaatccat
     2641 tttgattttc cttaatgaat aaatagggat gacttcttat gtatagtata ttgaattgaa
     2701 aaatcaagga aatttgaatt cgtttgtgga taacaataac aggtggatgg tgtctggaat
     2761 tgggttaatg atgaattaaa ttttgtttta aatcgatcaa attaatttgc aaaaatatta
     2821 aatcaattca atgatataaa ttaggtaaaa ttaatttatg agtgaatcat gctccaattt
     2881 tttgagaaag gtttgcaata ttcaggagaa aatatttccg ccgattctta tttaatgaga
     2941 aaaataattg aagcagcaac tccctctgct gataagggaa ctgtttctgc agaaaaaatg
     3001 ctgggcctgg tgaatccaaa tcagatagaa atagtatcgg gtagtgagaa cacatatcgt
     3061 ttaatagttc ataaaattaa agaggctaaa aagcaaatac ttattcaggc ttttgtatgg
     3121 gaaccagaaa ctcaggttgt caaagatatc cgaaatgcgc tttgtgacat caaacatgaa
     3181 gtcgaagtat ttcttctggt cgaccagttg gatcgtttgg cacaagtttt ttatcatggc
     3241 gaaatacttc ctaaaaaacc caaacatgat ccagcctgtt tgggtttaga taatttacct
     3301 cagaatatta aattgcatat aggtactcat gttcataatt ccattgcgtc caaccataat
     3361 aaagccatct ggattgatgg ggatgtcatt ttgacagggg ctaattttca gcaggaaaat
     3421 tatggtccta atgggtttca tgatgccgcc atgtttattc ctggcggcgc agcggaagcg
     3481 gttttttacg attttaaagc aatgtgggat aagcgcacca ataaatctga tgaactagaa
     3541 atttctccca tatttacagg ccaggagtca acagaatctt atagaaatac aaactcttgt
     3601 tctgtgctct atgtgacaaa tactatccgg cagaaatggg cgcttctccc tttttatagg
     3661 gcgcctttac ctgaagatcc attgaataac gcctacatgg ctgctattaa atatgccaaa
     3721 catactatcc gaatcgctgt tcccaattta aattcgccgg aaatcatcca tgccctggcg
     3781 gattttatta ataatagaaa tggccatgtc tatctattga tgggaaaggg attcaatgat
     3841 tttcgagaga gattttatgg cggtacaaat cagagttcga tagattatct tagttcacta
     3901 cttgatgagg acaggcaaaa taacttgcaa gttcgttggt ttcatcgtgt gaatgacaat
     3961 gaaatttcta taaaaaaaga ggttattcat atgaaattta tgtcaattga tgggcaggtg
     4021 gttatttacg gttcatcgaa tcttgatttg ttaagtttac ataatagcca tgaaacaaat
     4081 attgtaattg ataatgctga ttttgcacaa agagcaatag aacaattata tctacccatc
     4141 tttgaaacga gtataccagt attgatatcg cctaaaagaa cctgttttgt ttgttcttga
     4201 ttttaggttg ttttgatcaa gccaatatta attataatcc tgatacatga gttcgacata
     4261 aaaaataatt ccatttttat ccaaactcat gcaaattacc atcaatgtct ttaggtttta
     4321 gatgaataag cattgtcaat gctcaagtcc acacggaaga agttatataa attcaaataa
     4381 tctattggtt agctttttgc aatgcttgtt gctgtacttg tttaatctgc tcctgtaggg
     4441 ttgcttgtat ttgtttaatc tgatcttgcg tttgagaatt gactgtttga atttgttctt
     4501 gtaattgaga ttgaacttgt ttaatttgat tttgtaattg ggaatttaaa gcatccagtt
     4561 gttgctgttg ttttgtttgt atttcctgta attgagattg aatctgggca tttaattgct
     4621 gaatctgttc agctggtgtg ccggtcagtt gagcaggggt ttcatcagca taggctgggg
     4681 aaaacaagat tgctaacatc aaagatggaa gtgcatacaa tatcactttt ttcatttttt
     4741 atctccatta ttgaaaaagt ccaaattatc taatttattt tgctttaata ataatgctaa
     4801 tttaactata gcagaatcag agtgaaaaca ggttatacaa tgtctaatga aatgaagtaa
     4861 ctgacatttt tacgataggt ggaaaagaat catgaccaag gaattatgta tgcgttgtta
     4921 tatttcagga agagttcaag gggtatggtt tagggcttct gctaaaaaat tggctgaaca
     4981 attgatgata agtggatggg ctcggaattt agcggatggt cgtgttgagg tttttgcatg
     5041 tggaaaagaa gataagctgg aagaatttta tacctggttg caaaaaggac ctcttaatgc
     5101 aagagttgat gtatgtacac gagagaattt accttggaaa gattatataa gttttgatgt
     5161 gttataaaaa atcaacgcgc tctattatgg ggtaaatttt gtagtttgtt caaaaagttg
     5221 agcattccaa ttgctgaaaa tcaacatttc agtgaaaatg a
//
LOCUS       DAXLDT010000195         5256 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXLDT010000195 DAXLDT010000000
VERSION     DAXLDT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.195"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..443)
                     /gene="dotZ"
                     /locus_tag="ACF2SP_002770"
     CDS             complement(<1..443)
                     /gene="dotZ"
                     /locus_tag="ACF2SP_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 secretion system coupling complex
                     assembly protein DotZ"
                     /protein_id="HFD2360537.1"
                     /translation="MDEIKKDDELSQWLSTYGTITAERILGRYNISLPQDEILEAINI
                     PSSFYRHLLQIPLKNVLNGIVIQQASDYHVYAQKLLIDYLLSGESSKEPDSQGAGTRE
                     SLEDERQRLVQLGDEFHKLELEQDNLIASSQSSLMKTSIDWNTKL"
     gene            517..1140
                     /locus_tag="ACF2SP_002771"
     CDS             517..1140
                     /locus_tag="ACF2SP_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HFD2360538.1"
                     /translation="MLKRLDSDLQTLNDFLKTKTPDAWLNHAAANIPLLLLDHAHCER
                     KAAGTAINFISKYPEKAELVAIMAPLAREELLHFEKVIDIMKQKGIIYSPLQPSDYAS
                     NLHKHVTNKDGIERLCDQLIIGAIIEARSCERFSSIIPYIEDQDLARFYKTLVKSECR
                     HFEEYLHLAKFYGGSVDDRISEFLTIENAFILSDDVVFRFHSGLPPA"
     gene            complement(1229..1975)
                     /gene="ravY"
                     /locus_tag="ACF2SP_002772"
     CDS             complement(1229..1975)
                     /gene="ravY"
                     /locus_tag="ACF2SP_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavY"
                     /protein_id="HFD2360539.1"
                     /translation="MRSYPMSKPSNKMKEELEQIRKTEIENAIAKEREDLLAQRNRER
                     AEADDNLKKGRYIKDPRTGQSITLWESAVQKAEEVLNADLNSYMDARAAIMSLLKMYY
                     EMVKANAQTMKELRAGIGNTIMDWGVFPIKDYIGKKLTGNPEIDLPILQHEVSYTDDN
                     KLKIEPLTRSDKREEKGQLDKLFEGMIHMWLKNNDYTPDRDNPGQFVNSHGETLDKAT
                     FDKLKKDDQHGLNHFLTEYDPDLQFRPSRP"
     gene            complement(1972..2700)
                     /locus_tag="ACF2SP_002773"
     CDS             complement(1972..2700)
                     /locus_tag="ACF2SP_002773"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFD2360540.1"
                     /translation="MIDSVFISDLHLHPEDNEIQTRFDHFVNWARLSVKRIYILGDFF
                     HAWSGDDSINAWSTAIAEQIESLTQSGIEIYFMTGNRDFLLGSLFAKRTGWKILTEPT
                     VITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVNGVRKI
                     SLKNHHKSIEQMDIVTEAAIRHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSD
                     WDDIPKLLCYDNTKGFYFTQYDIN"
     gene            complement(2697..3392)
                     /gene="minC"
                     /locus_tag="ACF2SP_002774"
     CDS             complement(2697..3392)
                     /gene="minC"
                     /locus_tag="ACF2SP_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFD2360541.1"
                     /translation="MGENITNTQAFKLKGRLYTLTVLQVLNTDKDAFSQQFAEIVVRA
                     PKLFERTPVVFDLSSVNHLEFDLQELCQIARNHGMIPLAIQGGSPLHDTLAQCQGLGI
                     LNASTAHDKPIIEESSECQSIENFKTKLLTTPVRSGQQVVAKGADLIVTSSVSHGAEL
                     LADGNIHVYGTLRGRALAGISGDIEARIFCQSLDAELVSIAGFYRISDAIEPVNGPCQ
                     IYLANEHIVVEPL"
     gene            3464..5173
                     /locus_tag="ACF2SP_002775"
     CDS             3464..5173
                     /locus_tag="ACF2SP_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFD2360542.1"
                     /translation="MDKRWFEQYQNGVPHEIDASQYLSLISLFNESCNSYASRAAYSN
                     LGTEITYAELDSLSRNFAAYLQQLNLEKGARVAIMLPNVLQYPVAIFGVLRAGYVVVN
                     TNPLYTADEVIHQMNDSGAEVIIVLANFAKTIEKALPSMPSVKHVFVTQLGDLFPSFK
                     RFIVNFVVNHIKKLVPAFTIPHAVAFNYALSEGKQATLHRVELNHDDIAFLQYTGGTT
                     GVAKGAILTHGNLIANVMQAYTWISPLGVSNQDVIVTALPLYHIFSLTANCLTFMKIG
                     AKNILITNPRDMGHFIDQIKNSGFTAITGVNTLFNALLNHPKFKEIDFSKLKLALSGG
                     MALQKSVSLKWSEMTKTRVLEAYGLTETSPAVTINPMYLEGYNGSIGLPLPSTDVSIR
                     DESGKEVPIGTSGELCIKGPQVMPGYWKRPDETALVFTKDGFLKTGDIARMDEEGFIY
                     LVDRKKDMIVVSGFNVYPNEVEQIIGMHPGVLEVGVVGIVDEESGERVKACIVRKDST
                     LTAEQIINHCKEHLTAYKIPKVVEFFDELPKTNVGKILRRALKDQEKEQKYSVNKKTM
                     EPV"
BASE COUNT         1585 a          977 c          973 g         1721 t
ORIGIN      
        1 tccagttttg tattccagtc aatgcttgtt ttcattaagc tggattggct tgatgcaatg
       61 agattatcct gctctaactc caatttatga aattcatccc ctaactgaac taatctctgt
      121 ctttcatcct caagtgattc gcgagttccg gccccctgtg agtcaggctc tttgctgctt
      181 tcaccagata acaaataatc aattaacagt ttctgtgcat agacatggta atcacttgcc
      241 tgttgaatta caattccatt aagtacgttt tttaagggaa tctgtaacaa atgtctatag
      301 aaactagatg gaatgttaat cgcttctagt atttcatctt gtggcagtga tatgttgtat
      361 ctacctaata tacgttcagc ggtgattgtt ccataggtag aaagccattg actcaattca
      421 tcatcttttt tgatctcgtc cattttttat ccaatttaaa ttctttaaag tatgtgatat
      481 aagggctatt tacttgaaat tatagtgtat tttatcatgt taaaacgatt agatagtgat
      541 ttacaaacgt taaatgattt tttaaaaact aaaactccgg atgcctggtt aaaccatgca
      601 gctgccaata tacctctttt attgcttgat catgctcatt gtgaacgtaa agcagctgga
      661 acagcaatta atttcataag caaatatcca gaaaaagcag aattagtagc aattatggcg
      721 ccattggcaa gagaagaatt actgcacttt gaaaaagtca ttgatatcat gaagcaaaag
      781 ggaattattt attctccctt gcagccatca gactatgcat ccaatttgca taagcatgtc
      841 acaaataaag atggaattga gagactatgc gatcaattaa ttataggtgc tatcattgag
      901 gctcgatcgt gcgagcgctt tagctcaata attccatata ttgaagatca ggacttggcc
      961 aggttttata aaacattggt taaatcagaa tgcaggcatt ttgaagagta tttgcatttg
     1021 gccaagttct atggcggttc tgttgatgat cgtatttcgg agtttttaac tatagaaaat
     1081 gcgtttatct tatcagacga tgtggtattt cgctttcata gtggcttgcc tccggcttga
     1141 tgccttaaaa cagagtcaac agatttttca gcactagccc ataagtatta tatacaggct
     1201 agtgtaacct aatatttctc ttgtttaatt aaggtctgct tggtctgaat tgcaaatctg
     1261 gatcatattc tgttaaaaag tgattaagtc catgctgatc gtcttttttt aatttatcaa
     1321 acgttgcttt gtctaaggtt tcaccgtgtg aattaacaaa ttgaccagga ttatcacggt
     1381 caggggtata atcattattc tttagccaca tgtggatcat accctcaaat aatttatcaa
     1441 gttgcccctt ttcctcacgt ttgtctgagc gagtcagtgg ttcaattttc aacttattgt
     1501 catcagtata actcacttca tgttgcaaaa ttggtaaatc aatttcagga tttcccgtga
     1561 gtttttttcc aatataatct ttaatgggaa agacacccca atccataatc gtgttaccaa
     1621 tccctgcgcg caattccttc atagtctgag catttgcctt gaccatttca taatacattt
     1681 ttaataaact catgatcgca gcacgagcat ccatataaga atttaaatca gcattcaaaa
     1741 cttcctctgc tttttgcact gcactttccc agagagttat tgattggcct gtacgaggat
     1801 ctttgatata tcggcctttt tttaggttgt catcggcctc agcgcgttct cggtttcttt
     1861 gggcaagtaa atcttctcgc tcttttgcta tagcattttc tatttcagtt tttcgaattt
     1921 gttcgagttc ctctttcatt ttatttgatg gtttagacat tgggtaactc ctcaatttat
     1981 atcatattgg gtgaaataga atccttttgt attatcataa cacaataatt tgggtatgtc
     2041 atcccaatca cttagaacat accttgtcag tattttttct tcataagtgt aatttgttat
     2101 gccaggttta tgcgtgtgtc catgaatcag tatttctgtg ttattttcag acatatgtcg
     2161 aattgcagcc tctgtaacaa tatccatctg ttcgattgat ttatggtgat tttttagact
     2221 gatcttgcga actccgttaa ccaatcgttt gcgaaatgat aaaggaagtc ttaaaaaaag
     2281 aaatgagaac cacctgtttc tcgttaataa tcggaatcgt tgatgtgatc tgtcattagt
     2341 acaataacga tcgccatgaa ccagcaagat cttatgattt ccaagtgtaa taacggtcgg
     2401 ctctgttaat attttccaac ccgttcgttt ggcaaatagt gagccaagta aaaaatcacg
     2461 atttccagtc ataaaataaa tttcaatgcc actctgagtc aatgattcta tttgttctgc
     2521 aatagcagta ctccatgcgt taattgaatc atcacctgac caggcatgaa aaaaatcacc
     2581 tagtatgtat atccttttta cagatagccg agcccaattt acaaaatgat caaatctggt
     2641 ttgaatttca ttatcttctg gatgcagatg taaatcggat ataaaaacgg agtcaatcat
     2701 aatggttcaa caacgatatg ttcatttgcc aagtagatct gacaaggccc attaactggt
     2761 tcaatagcat cacttattcg atagaaaccc gctatggata ctaattcagc atccaaagac
     2821 tgacaaaaaa ttcttgcctc gatatccccg gatattcctg ctaatgctct gccgcgtaat
     2881 gttccataca catgaatatt tccatcagct aataattctg ccccatggct aactgaagaa
     2941 gtcactatca aatcagctcc cttggcaacg acttgttggc ctgaacgcac tggtgtcgtt
     3001 agtaattttg ttttgaaatt ctctatactt tgacattctg aggattcttc gattattggc
     3061 ttatcatgtg ctgttgacgc attgagtatg cccaaaccct gacattgagc cagagtatca
     3121 tgtaaaggac taccgccttg aattgccaaa gggatcatac catgattacg tgcaatttga
     3181 cataattcct gtaaatcaaa ttcgagatga ttgacagaag acaagtcaaa aacaacgggt
     3241 gttctttcaa ataatttagg cgctctaaca acaatttcag caaattgttg agaaaaagca
     3301 tctttatcag tgttcaaaac ttgcaaaacg gttaaagtat acaaccttcc cttaagctta
     3361 aatgcttgtg tattagttat attttcaccc attagcatga tatccattag agagtttttt
     3421 gtttatacta tcatgaggaa aacaataaca gaaggatatc aaagtggata aacgatggtt
     3481 tgaacaatac caaaatggag taccacatga aattgacgca agtcaatatt tatccctcat
     3541 atctttattt aatgaatcat gtaactctta tgctagcaga gctgcctatt ccaatttagg
     3601 cacggaaatt acttatgctg aactggacag tttaagcaga aatttcgctg cttatctgca
     3661 gcaattgaat ttggaaaaag gggcaagagt agcaataatg ctgcctaacg ttttacaata
     3721 tcctgtagcc atctttggtg tgttgcgtgc aggttatgtc gtggtaaaca ctaacccttt
     3781 atacacagcg gatgaagtaa tacaccaaat gaatgactcg ggggcagagg tcattatcgt
     3841 gctggctaat tttgcaaaaa cgattgaaaa agcattaccg tcaatgcctt ccgttaagca
     3901 tgtgtttgtt acgcaattgg gcgatttatt tccttcattc aagagattca ttgttaattt
     3961 tgtagtgaat catataaaaa aattagttcc tgccttcaca attcctcacg ccgtggcttt
     4021 taactatgct ttatctgaag ggaaacaggc aacgctccat cgagttgaac tgaatcatga
     4081 tgatattgcg tttctacaat atactggcgg tactactggt gttgcaaaag gcgctatttt
     4141 gactcatggc aatctaatag ccaatgtgat gcaagcatac acctggatat ctcctctggg
     4201 agtcagcaat caagatgtta tagtaactgc acttcctttg taccatattt tttccttgac
     4261 ggccaattgc ctcactttta tgaaaattgg cgcgaaaaat attctgatca ccaatccacg
     4321 tgatatggga cactttatag atcaaatcaa aaattctggt tttacagcaa taactggggt
     4381 taatactcta ttcaatgcat tgctgaacca tcctaaattt aaagaaattg atttcagcaa
     4441 attaaaactg gcattatcag gcggtatggc attgcaaaag agtgtgtcct taaaatggtc
     4501 tgaaatgact aaaacacgtg ttttggaagc ttatggatta accgagacta gccctgccgt
     4561 aactataaac ccaatgtacc ttgaaggcta taatggcagt attggcttac ctcttccttc
     4621 aactgatgta tctattcgtg atgaatccgg gaaagaagtg cctattggta caagtgggga
     4681 gttgtgcatt aaaggaccac aagtgatgcc tggttattgg aaaaggcctg atgagacagc
     4741 cttggtattt actaaagacg ggttcttaaa aactggcgat attgcacgta tggatgaaga
     4801 aggctttatc tacctggtcg atagaaaaaa agacatgatt gttgtttcag gtttcaatgt
     4861 ttatccaaat gaggtagaac aaataatagg aatgcatccc ggtgtattag aagtaggcgt
     4921 tgtaggtatt gttgatgaag aatctggtga acgggttaaa gcctgtattg taagaaaaga
     4981 tagtacattg acggctgaac aaattatcaa tcattgcaag gagcatttga ccgcttataa
     5041 aatccccaaa gttgtcgaat tctttgacga acttcctaaa acaaatgtgg gcaagatttt
     5101 acgtcgtgct ttaaaagacc aagaaaagga acaaaaatac tcggtcaata aaaaaacaat
     5161 ggagcctgtt taggtgtaat tggagtattt tgggtgctat ttgtccttaa tgctcctctt
     5221 ctaaaagtat tctgaatata gcctcagata agttcc
//
LOCUS       DAXLDT010000196         5255 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXLDT010000196 DAXLDT010000000
VERSION     DAXLDT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.196"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1234
                     /locus_tag="ACF2SP_002776"
     CDS             <1..1234
                     /locus_tag="ACF2SP_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFD2360543.1"
                     /translation="LLKSKKRYFKNAISLLVILVPITLFTTAIIGLIGFVNLAWTMSR
                     YQAYILLVLVGYILARGLIFDALELFSEWMISSLKNGWLWIEVFLKPIDKILRIVLFL
                     TSVLILFQLFGWYSDSLVVTSLEKVVQYSILNITGIHITVASIIEFLILLAVLVWAAK
                     WTREFCFRWLYKNTKDVGIRHSLSVFTQYAIILLGTFITLHVLGLDFSGMSMILGGLA
                     VGMGFGLRDFASNIVGGIMLLIERPVREGDLITIGEYEGQVAHIGIRSMRVSSWDNTQ
                     VLIPNAETFNKPFTNWTHQDGIIRTVIPIKVSRADDPVMIQQLILDVLAIIPEIVPDP
                     PAQVFLKKIDTALIEFEARYFINIQNHNRVEVRSKVLFAITAQFKAAGIQPPIEPITV
                     ELKESSSDFSLKKQTTEE"
     gene            1197..1871
                     /gene="mutH"
                     /locus_tag="ACF2SP_002777"
     CDS             1197..1871
                     /gene="mutH"
                     /locus_tag="ACF2SP_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFD2360544.1"
                     /translation="MILALKNKPPKNEKELLEQCQRIEGLSFAQLALELGLFTPLNPV
                     HRKGWVGQALELALGADAKNESLPDFKQLGIELKTIPINKAGKPAESTFIVSIPLLTI
                     HQQSWESSQCYAKLKKVLWIPIEGDTDIPFPHRRIGCGFLWSPNKDQESVLAEDWNYL
                     TNQIVLGQLETLNSSSGEYLQVRPKAANGKSLCYGYDAEGNRIRTLPRGFYLRSCFTS
                     VILSQS"
     gene            complement(1944..2984)
                     /locus_tag="ACF2SP_002778"
     CDS             complement(1944..2984)
                     /locus_tag="ACF2SP_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360545.1"
                     /translation="MKTIFANKELLYNQYKKMFLEQISDINKLKECIKARYDIKELPD
                     EILFKLLTNNHKKIYDFIGTHNQNRIRYFFGNFDNFDPRDNQISVLDRFILDSVHFYI
                     TSSLLMQLHKEYQENAMEIDKAWQKEIQAHAHFGPLPTIAIIGNLIKSNKNHVFKDLQ
                     FPANKNIQLFQLNKLSIKLNDQVFAFWLPKASEAQVSLSNFTRNTIFVCIATAAILAI
                     GVSVFVFSTSSAAGLAVIFNPASLGIMIALLACATLMLGISSLYSSTTTHDSTIEHPE
                     NRTPEKPYHSHNIRKDKIHSSKAKPEEQLPYHDLKWLLFNRKSHTLRESIVTPATASE
                     QLSSMCTNRQKL"
     gene            complement(3183..4019)
                     /locus_tag="ACF2SP_002779"
     CDS             complement(3183..4019)
                     /locus_tag="ACF2SP_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360546.1"
                     /translation="MGKVNAVGMAEVGVERAKRKEALRVLTSSPFNLDNKEHTQALEK
                     YIKQFELSVNQMYLYQAMSTALTAWGGSWIVGRFLPIPEFANHLLAACLYLGVSGYIL
                     ESFSMTDFYEQLKEMKTLYNWCLKNNKENYSTDIDNTNNLLYPDIQRMIKLVAPLCST
                     DFMIAWPKEVKTTETQSGILSNTLSIGYSIVSAPFSLFSTASKPSQDQVQRIKDLKVQ
                     VETRGLDLGVFAGFEQAVKYFATNSDFRDLLKAKLTQPVNSFKNLLQTTIPGMLAGSN
                     SK"
     gene            4165..>5255
                     /gene="legK1"
                     /locus_tag="ACF2SP_002780"
     CDS             4165..>5255
                     /gene="legK1"
                     /locus_tag="ACF2SP_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector kinase
                     LegK1"
                     /protein_id="HFD2360547.1"
                     /translation="MPRTMFFSVDAANLQNLQITNQKAFAALESYLNDNDSPGAWNNN
                     QGYKYTHKDVTYCFSFNQSLVRRPRKSDQKKHTFEIFDPNMNPLGKGAYGIVYPILGT
                     IQFEFGNAIKRPPKNKLVKIQNHSERDQSYSVLREYQGLLQSGHIAVKPPIFVENKES
                     KFSYLIMEKAEGIVLEKFLNPVKRLDLSEDVPELNLINRIEITFAILKAIKEQVTDKH
                     LIHRDIKPGNIIIDFSKSPPIAKVIDFGFVLRASEQDYRRCGTRAYRAPESFNTQALY
                     TAKADVWSTGRILSYLWGDKYTNYYISRDKGLDYVIEKSRNELLFSDPELELYLTKED
                     KSKIRSCLSTMLIVDPGERGSIDKAIKQFS"
BASE COUNT         1634 a          876 c         1014 g         1731 t
ORIGIN      
        1 attactgaaa tcaaagaaaa gatattttaa aaatgcaatt tctttattgg tcattttagt
       61 tcctataacc ttatttacaa ccgcaattat tggtttaatt ggctttgtga atctggcatg
      121 gactatgagt cgttatcaag cttatattct gttggtttta gtaggttata ttctcgcaag
      181 aggtttgata tttgacgcat tagagctttt ctctgagtgg atgatctctt cattgaaaaa
      241 tggttggtta tggatagagg ttttcctcaa accaatcgat aaaatcttgc gtattgtctt
      301 gtttttaaca agtgtattaa ttttatttca gttgtttggt tggtactctg attcactggt
      361 tgtgactagc ctcgaaaaag tagttcaata cagtattctt aatattacag gtatacatat
      421 cacagtcgcc agtatcattg aatttcttat tctgcttgct gttttagtat gggcagcaaa
      481 gtggacaaga gaattttgct ttcgatggct atataagaat accaaagatg tgggtatacg
      541 ccatagttta tctgtattta cacagtacgc aatcatatta ctgggtactt ttatcactct
      601 tcatgtcttg ggactggatt ttagcggtat gtcgatgata cttggtggac ttgctgtcgg
      661 tatgggtttc ggtttaagag attttgcttc aaatattgtt ggaggcatta tgcttttaat
      721 tgaaagaccc gtaagggaag gcgatttgat caccataggt gaatacgaag ggcaagtggc
      781 tcacataggc attcgctcga tgcgcgtatc ttcatgggat aatactcagg ttttgattcc
      841 gaatgctgaa acattcaata aaccttttac aaactggact catcaagatg gtattattcg
      901 tacggtcata ccaataaagg taagcagagc cgatgatcca gtgatgattc aacagttaat
      961 acttgatgta ttggcaatta ttccggaaat tgttccagac ccccccgcac aagttttttt
     1021 aaagaaaatc gatacagctt taatagaatt tgaagcacgg tattttatca acatccagaa
     1081 tcataaccgg gttgaagtaa ggtcaaaagt cttgtttgcc ataactgcac agtttaaagc
     1141 ggcaggaata cagccgccaa ttgagcctat tactgttgaa ctaaaagaga gctccagtga
     1201 ttttagcctt aaaaaacaaa ccaccgaaga atgaaaaaga acttttggag caatgccagc
     1261 gaattgaggg gttgagtttc gctcagttag ctcttgaatt gggtctattt acccctttaa
     1321 accccgttca tcgaaaagga tgggtcggac aagctctcga gttagcttta ggtgctgatg
     1381 caaaaaatga gtcgctacct gatttcaagc agttgggcat tgagttaaaa acgataccta
     1441 ttaataaagc agggaaaccg gccgagtcca catttattgt ttctattcca ctactgacca
     1501 tccatcagca aagttgggag tcttctcaat gttacgctaa attaaaaaag gtattatgga
     1561 ttcctataga gggagataca gatattccct ttcctcacag aagaattgga tgtggatttt
     1621 tatggtctcc gaataaagat caggaatctg ttcttgcaga agattggaat tatttaacaa
     1681 atcaaatcgt tctgggacaa ttagaaacat taaattcctc ttcgggagag tatcttcaag
     1741 tgaggcctaa ggctgccaat ggaaaatctt tatgttatgg ttatgacgca gagggtaatc
     1801 gtataagaac gttaccacga ggattttatt tacgctcctg ttttacatct gtaattttaa
     1861 gtcaatccta attttaagga taaattaagc accatctgtt taccggtatt atgataaatt
     1921 ttacagatat tagggaaagc ttattatagc ttttgtctat tagtacacat ggacgataat
     1981 tgttcagatg ctgttgccgg agtcactatt gattctctta atgtatgtga ttttctgtta
     2041 aataataacc atttgagatc atggtagggt aactgctctt ctggtttggc cttactagaa
     2101 tgtattttat ccttgcgaat attatgagaa tgataaggtt tttctggtgt tctattctcc
     2161 ggatgttcta ttgtactgtc atgtgtagta gtggaagaat aaagagagct tatacccagc
     2221 attagcgtgg cacatgctaa caatgcaatc attattccta gcgatgcagg attaaaaata
     2281 acggctaaac cagctgctga gcttgtagaa aatacaaaga cacttacgcc tattgctaaa
     2341 atagcggcag tggcaataca aacaaaaatg gtattgcgag tgaagttgga tagtgatact
     2401 tgtgcttcac ttgctttggg taaccagaac gcaaagactt gatcattaag cttaattgat
     2461 aatttattta attgaaagag ctgtatgttt ttgttcgcag ggaattgtaa atctttaaag
     2521 acatggtttt tatttgattt aatcaagttt ccaatgattg ctattgttgg taatggaccg
     2581 aaatgggcat gggcttgaat ttccttttgc catgctttat caatttccat tgcattctct
     2641 tgatattctt tgtgtaactg cattaataaa ctgctagtga tataaaaatg aacgctatca
     2701 agaataaatc gatctaaaac gctaatttga ttatcacgtg gatcaaaatt atcaaaatta
     2761 ccgaaaaaat atcggattct attttgatta tgtgtcccta tgaaatcgta aattttttta
     2821 tgattattag ttaataattt aaaaagtatt tcgtcaggaa gctcttttat atcatacctt
     2881 gctttaatac attcttttaa tttgttgatg tcagatatct gctctagaaa catttttttg
     2941 tattgattgt aaagcaattc cttgtttgca aaaatagttt tcatcttgag aaacctttgt
     3001 ggagtaaatc aatgacatta tttgctgtta atcatcttca tattggccgt aatgatgatt
     3061 atgcctccat tctttaatgc taatgccaac ctttaataat gcttaaggtc ggcaagaaaa
     3121 acatcagttc tttacctaat acaaccgtta ggtaatattt aagcatgagg gatctattac
     3181 tattattttg aattagatcc tgccaacatt cctggtatgg ttgtttgcaa caaattttta
     3241 aatgagttga caggttgagt tagcttggct ttcaatagat ctctaaaatc agagttcgta
     3301 gcaaaatact tgactgcctg ctcaaaacca gcaaaaaccc ctaagtcaag acctcttgtt
     3361 tctacctgta cttttaggtc tttaattcgt tgcacttgat cctgactcgg tttactggct
     3421 gttgagaaca gtgaaaatgg cgctgatact atagaatagc ctatggacaa tgtgttggat
     3481 aggattccac tttgagtttc tgtggttttt acttctttag gccaggcaat cataaaatca
     3541 gtagagcata aaggggcgac tagtttaatc atgcgctgga tatcaggata caaaaggtta
     3601 ttggtattat cgatatcagt gctatagttt tctttgttat tttttagaca ccaattatag
     3661 agtgttttca tctctttcaa ttgttcataa aaatcggtca tgctaaatga ttcaagaatg
     3721 taaccagaaa cgcctaaata gagacaggct gcaagaaggt gattcgcaaa ttcaggaata
     3781 ggcaaaaacc ttccaactat ccacgagccc ccccaagctg ttaatgcagt actcattgct
     3841 tgatagaggt acatttgatt gacacttaac tcaaattgtt tgatgtactt ttccaatgcc
     3901 tgagtgtgtt ccttattatc caggttgaag ggagatgacg tcagaactct tagggcttct
     3961 ttccttttgg ctctttcaac tccgacttca gccattccaa ctgcgtttac cttacccaca
     4021 attctctcct tgaaatcaaa atttcatttt atactataaa tttaaaaaat aatgaattag
     4081 attttagcta agagttcaat taatttaact taataaaaaa gacgaggtat aataaaaccg
     4141 atcaatcaaa ttgaagtcaa tatcatgcct cgtacaatgt ttttttccgt tgatgcagct
     4201 aatctccaaa atttacaaat tacaaatcaa aaagcttttg cagcgttgga gagttatctg
     4261 aatgataatg attcaccagg tgcctggaat aataatcaag gatacaaata tactcataag
     4321 gatgttactt attgcttctc atttaatcaa tccttagtga gacggccaag aaaatcggat
     4381 caaaaaaaac atacatttga aatttttgac cctaatatga accctttagg caagggagcg
     4441 tacggtattg tgtatcccat cttaggtaca attcagtttg aatttggaaa tgccattaaa
     4501 agaccaccaa agaataaatt agttaaaata caaaaccata gtgaacgcga tcaaagttat
     4561 tctgtactac gtgaatatca agggcttctt caatcagggc atatagcggt taaaccgcca
     4621 atatttgtag aaaacaaaga gagcaaattc agttacttga ttatggaaaa agcagagggt
     4681 atcgttttag agaagtttct aaatcccgtt aagcggcttg atttgtctga agatgtacct
     4741 gagcttaatc ttattaatcg gattgagatt acttttgcca ttttaaaagc aattaaagag
     4801 caggtgacgg ataagcattt gattcatcga gatataaaac caggaaatat tataatcgat
     4861 tttagcaagt cgcctccgat agctaaagta atcgattttg gttttgtgct tagggcgtca
     4921 gaacaggatt atcgtcgttg tggaacccga gcatacagag cgcccgaatc gttcaatacc
     4981 caggcattgt acacagcaaa agctgatgtt tggtcaacag gcaggatatt aagttatttg
     5041 tggggtgata aatatactaa ttattatatc tctcgtgata agggtttgga ctacgtgatt
     5101 gagaagtcaa gaaacgaact actcttctct gacccagaat tggaactcta tttaaccaag
     5161 gaagataaaa gtaaaatcag atcgtgccta agcacaatgc ttatagttga cccaggggaa
     5221 agaggatcaa ttgataaggc tatcaagcag ttttc
//
LOCUS       DAXLDT010000197         5217 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXLDT010000197 DAXLDT010000000
VERSION     DAXLDT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.197"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            52..744
                     /locus_tag="ACF2SP_002781"
     CDS             52..744
                     /locus_tag="ACF2SP_002781"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFD2360548.1"
                     /translation="MTMKFPFQIIDLTHPVSPDSPGWDLDCGFIHSNFIDYEDCTSEI
                     KFRVQQIHSPLGIGTHMDAPAHCIPGAASITDLDLHRNYLLTQSVLINVSHQTDEHYQ
                     ITKQDILDFEARYGRLKKNVFVLFYTGWEKHWPNPDKYRNNLVFPSISAHAAQLLLER
                     NIAGIGIDTLSPDTADSGYPVHQLLLQAGKYIIENVANANSLPPIGAWTLALPWKIIN
                     ATESPVQLIGLV"
     gene            complement(795..2213)
                     /locus_tag="ACF2SP_002782"
     CDS             complement(795..2213)
                     /locus_tag="ACF2SP_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HFD2360549.1"
                     /translation="MDKNHNTKKILFKNRFVILGFGCVGQALMPLIFEKFDIKPSQVT
                     IIAAEGTKVDVAQQYGVSFKLQQITPQNYLEVIGSTLEENDFLIDVSIGISSLALIIL
                     CNQKGALYINAATEPWKEEFVMEKMALNRRTNYSLREEVLRLKDKTQKTALITHGANP
                     GLVSHFIKEALLNIAKDNGLTINRPKNAAEWANLAMTLGIKVIHVAEQDSQVTYPPKS
                     PGEFVNTWSANGLILEGLQPAEIGWGTHEAHWPHDAYSHSNGPQCAIYLSRPSAGVMV
                     RSWTPTLGAFHGFLITHAETISLTNFLTLKNGSELLYRPTVHYAYNPCPDARLSIFEL
                     KSNEWKPQNKNRLILNEIIDGCDELGVLLMGNQRGAYWYGSTLSIQEARQIAPYNNAT
                     SLQVVASMISGIIWAIEHPDEGIVEPEEVDHQYIIDIAKPYLGKVGGYYTDWTPLKNR
                     GELYPEEVDLSDPWQFFNIRVN"
     gene            2491..2940
                     /gene="trmL"
                     /locus_tag="ACF2SP_002783"
     CDS             2491..2940
                     /gene="trmL"
                     /locus_tag="ACF2SP_002783"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFD2360550.1"
                     /translation="MIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPLGFTLEDKQM
                     RRAGLDYHQWASILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPE
                     SCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMGFNM"
     gene            complement(3098..3748)
                     /locus_tag="ACF2SP_002784"
     CDS             complement(3098..3748)
                     /locus_tag="ACF2SP_002784"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFD2360551.1"
                     /translation="MLDVINFKDYRSILCLNGDLPAPSFFHKRKLPVIAADGAANVLY
                     NLGVFPDLITGDLDSIQPALLENHSFLHLPDQGSTDYQKAMHYLQTHDLLPAIVVGIN
                     GGYLDHILNNINIFMDTNCLLYSPPIKGFVVNEKLRVNFILPVQTKISLIGIPEVVLS
                     SEGLKWELKHSHLSFPGKNSCFNRTQTAEISLEVHQGAALVLIYEDAIEDAGVSSL"
     gene            complement(3732..3998)
                     /locus_tag="ACF2SP_002785"
     CDS             complement(3732..3998)
                     /locus_tag="ACF2SP_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HFD2360552.1"
                     /translation="MSIVIFSGIVAFITSFIGLLPQIVKSLKTKSTEDLSMMMLVNYL
                     VCSLAWIIYGSSTNSFFVTSSNVVGLIVSFLLILLKRRYDARCN"
     regulatory      complement(3985..4088)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4230..5174
                     /locus_tag="ACF2SP_002786"
     CDS             4230..5174
                     /locus_tag="ACF2SP_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LirA/MavJ family T4SS effector"
                     /protein_id="HFD2360553.1"
                     /translation="MTITHTAAGGITMTVELIRNGETDTNDYEEHLDSFGYETLEKSL
                     KEDAKSICQFFCNEELVLQGLKKLVTQFYQFNQPEKKTLRQFFDDWGSKNHFNHEAQP
                     NDKIPYETSPNFHFPNHTPILHGELTADLFNNVLLKQGYLSADLGAGPRHGKWAHTIQ
                     FFILEEARKTGQLKLHSNTVCEFIKKISQIHGPYESFSLWSILFDSFDETFTCPNIIT
                     QTLTSPWDNSKEALFLADKLNHFQKKFEKAASAENNYNAYANKKYMSRIDKANYVKYK
                     DKCAILWFAPQDKKTIPKPSCQEELKQNQLSFLKLSLL"
BASE COUNT         1728 a         1042 c          907 g         1540 t
ORIGIN      
        1 tcctgattct ttttatgata atttaattga aataatccat cgctctctca aatgactatg
       61 aaatttcctt ttcaaataat cgatttaaca caccctgttt cacctgactc ccctggttgg
      121 gatctggatt gtggatttat ccatagtaat tttattgatt atgaagactg tacatcggaa
      181 atcaaattca gagttcagca aatacactca cccttgggga ttggaaccca tatggatgct
      241 ccggctcatt gtattccagg agctgcttct attactgatc tcgatctgca ccgtaattac
      301 ctgctcacac aaagtgtgct gattaatgtt agtcatcaaa cggatgaaca ttatcaaatt
      361 acaaaacagg atattctgga tttcgaagcc agatatggga ggttaaaaaa aaatgtgttt
      421 gtgctttttt atactggctg ggagaaacat tggcctaatc ctgacaaata tcgcaacaat
      481 ttggtttttc caagcattag tgcccacgca gcccaattat tgcttgaaag aaatattgct
      541 ggcattggga tagacacact ttctccagat actgcagact caggttaccc tgtgcatcaa
      601 ctcttattac aagcaggtaa atatattatt gaaaacgttg ccaatgcgaa ttccctgcca
      661 cccattggcg cctggaccct ggctttacca tggaaaataa tcaatgctac ggagtcacca
      721 gtccaattaa ttggactggt atgataatgt atagttcatt gattaatact aattattaca
      781 gtacgttact ccttttaatt aactcttata ttgaaaaatt gccagggatc agataagtcc
      841 acttcttcag gataaagttc ccctctattt tttaaagggg tccaatccgt gtaatagccg
      901 ccaacttttc caagatacgg tttggcaata tcaataatat attggtgatc aacttcctct
      961 ggttctacta taccctcatc aggatgctct attgcccaga ttattcctga aatcatactg
     1021 gcgacaactt gtaaactggt tgcgttatta tagggggcaa tttgccttgc ttcctggata
     1081 gaaagagttg aaccatacca atacgcccct ctttgattac ccatcaacaa aacacccaat
     1141 tcatcacatc catcaatgat ttcgtttaaa atcaggcgat tcttgttttg tggtttccat
     1201 tcattacttt ttaattcgaa aatggagagc ctggcatctg gacaaggatt ataggcgtag
     1261 tgaactgttg gcctatacaa taattcagat ccatttttta atgttaaaaa attggtaagt
     1321 gaaatagttt ctgcatgagt aattaaaaaa ccgtgaaaag cacccagtgt tggcgtccat
     1381 gaacgtacca ttacaccagc actagggcga gacagataga tcgcgcattg tggtccatta
     1441 ctatgagaat aagcgtcatg gggccaatga gcttcatgtg tcccccaccc gatttcagca
     1501 ggttgcagtc cttccaaaat cagaccatta gcggaccagg tattaacgaa ctcgcctggt
     1561 gattttggag gataggtaac ttgagaatct tgctctgcaa catggattac tttaattccc
     1621 aaagtcatgg cgagattagc ccattctgct gcatttttag gtctgttaat ggtaaggcca
     1681 ttatcttttg caatattcaa caaggcctct ttaataaaat gtgaaacgag acccggatta
     1741 gccccgtgag taattaaggc tgttttttga gttttatcct ttaagcgaag gacttcttca
     1801 cgcaaagaat aattggttcg gcgattaagc gccatcttct ccataacaaa ttcttctttc
     1861 cacggctcag ttgcagcatt aatatacaat gctccttttt gattacatag tataatcaaa
     1921 gcaagacttg atatgccgat tgacacatca attaaaaaat cgttttcttc caaagtacta
     1981 ccaatcactt caagataatt ttggggagta atttgttgta acttgaatga aactccgtat
     2041 tgttgagcaa catcgacctt tgtcccttct gcggcaatga ttgtcacttg cgatggtttg
     2101 atatcaaatt tctcaaagat taaaggcatt aatgcctgac caacgcaacc aaatcctaat
     2161 atgacaaaac gatttttaaa caagattttt tttgtattat gattcttatc catgatgaat
     2221 ccttaacttc ccggcccagt ttaatttgaa tttttaatac tcaacaactt ttcaacaatg
     2281 atcataccca acagacatca aaaactttca tatggcatac taatattgta gtagctatgt
     2341 tgaattcaga cagcctgttg agtaaaaaat tagacacatc tactccctgc ttgattttta
     2401 aagtatgtag taaaattgca tcctgccact ctattcctgg ctttgaataa atcaattaaa
     2461 atggcggcca gaatgctact ggaaaatcac atgatccata ttgcgcttta tcaaccagaa
     2521 attccaccga acacgggcaa tattattcga ctctgcgcca atactggagc tcaactgcat
     2581 ttgattcatc cattaggctt tactttggaa gataaacaaa tgcgacgagc cggtcttgat
     2641 tatcatcaat gggcctcaat attgcattat gagaattggc aaacattcct gcagtctcat
     2701 tcacaacgac gattattttg ctgtagcgca aaaggaaaag tgaagtatag tgacgtcgat
     2761 tataaggcag atgacatctt attatttgga cccgaatctt gcggcttacc tcaacaaata
     2821 ctgaaccaat attcaacaat acggattccc atgagagaaa ataatcgtag tctcaactta
     2881 tcaaatgctg tagcaatcat attgtacgaa gcatggagac aaatgggttt caacatgtaa
     2941 gcgttaactt ggtaatccac ctgatagctc ttacagcaat ggaagagacg ggttatgcaa
     3001 gcagtactta atataaacag gacttacgaa aaaccacaag accgaggcaa aaattttttt
     3061 taacaaagag attggggagg gcgtaagtcc ttattcctta taaagaggaa actccagcat
     3121 cctcaatagc atcttcataa atcaaaacaa gggcggcgcc ctggtgaact tccaaactaa
     3181 tttctgctgt ttgtgttcga ttgaaacagg aattttttcc aggaaacgaa aggtgtgagt
     3241 gctttagctc ccacttcaaa ccctcagaag acaggacaac ctcagggata cctattaacg
     3301 agattttagt ctggacaggt aaaataaaat taaccctcaa tttttcattg acaacaaaac
     3361 ctttaatggg aggcgaatat aaaaggcagt ttgtatccat aaaaatgttg atgttattca
     3421 gaatgtgatc caaataccca ccatttatcc ctacaacgat agcaggcaat agatcatgag
     3481 tttgtaaata atgcatggcc ttttgataat ccgtactccc ttgatctggt aaatgaagaa
     3541 aagaatggtt ttccaacaaa gcaggctgaa tagaatccag atctccagta attaagtcag
     3601 gaaagacacc aaggttgtat aacacattag cggcaccatc agcagcaatg accggcagct
     3661 tccttttatg aaaaaaggaa ggagcgggca aatcaccatt aagacataaa atagaacgat
     3721 agtctttaaa gttaattaca tcgagcatca taacgtcttt ttaataaaat cagcagaaaa
     3781 ctcactatca aacccactac attggaactc gttacaaaaa atgagtttgt gctgcttcca
     3841 taaatgatcc aggccagcga acaaactaga taattcacta acatcatcat ggaaagatct
     3901 tcagttgatt tggtttttaa agatttaacg atttgcggta aaagtccaat aaacgaggta
     3961 ataaaagcaa ctattccaga aaaaataaca attgacacat tttcctccgc tcagactaat
     4021 gagatcaggt tcaaagggtt gggtgatcct ctcagccaac ttatcaattg gcacccctaa
     4081 tgttggactt atattttatc acgaaatgag cagaccgcaa tttaatagca agaaaatact
     4141 cctttctttt ttcatcagtt tatgattaat taataatcct ttagttcaaa aaaggaatta
     4201 agctcctcaa ataattctca aatcggtatt tgacaataac tcatacagca gctggaggca
     4261 tcactatgac agtagaactt attcgaaatg gagagacaga taccaatgat tatgaggaac
     4321 acctggattc ttttggatac gaaacattag aaaaatcctt gaaagaagat gccaagtcta
     4381 tatgccaatt cttttgtaat gaagagctgg ttttacaagg tttaaaaaaa ttggtgacac
     4441 aattttatca gtttaaccaa cctgagaaaa aaacattaag acaatttttt gatgactggg
     4501 gaagtaaaaa tcattttaac catgaagctc agcctaatga taagatacct tatgaaacca
     4561 gccccaattt ccatttccct aatcacactc cgattttaca tggtgaatta actgcagact
     4621 tattcaataa cgttcttcta aaacagggtt acttatctgc tgatcttggc gcaggcccca
     4681 ggcatgggaa atgggcgcat accattcaat tttttattct ggaagaagcc agaaaaaccg
     4741 gacaattaaa gcttcatagc aatacagtct gcgaattcat taaaaaaatc tctcaaatcc
     4801 atggcccata cgaatcattc agcttatgga gcattctctt tgattcattt gacgaaacct
     4861 ttacctgtcc taatataatt actcaaactt tgacatcacc atgggataat tcgaaagagg
     4921 ctctattttt agctgataaa ttaaaccatt tccaaaaaaa atttgaaaaa gccgcctctg
     4981 ctgaaaataa ttacaatgcg tatgccaata aaaaatacat gagcagaatt gataaagcga
     5041 actatgtcaa atacaaagac aaatgtgcaa tactgtggtt tgcgccacag gataaaaaaa
     5101 ctatcccaaa accttcctgc caagaagaat tgaaacaaaa tcaattaagc tttttaaaat
     5161 taagcttatt ataagaggat atgactttat atcgataaac ttaaataaaa catccct
//
LOCUS       DAXLDT010000198         5158 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXLDT010000198 DAXLDT010000000
VERSION     DAXLDT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.199"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            167..739
                     /locus_tag="ACF2SP_002787"
     CDS             167..739
                     /locus_tag="ACF2SP_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFD2360554.1"
                     /translation="MLHNTKINKLAVFCGSNTGYSEVYKQSAENMADVLSSNGITLVY
                     GGSKSGLMGIIANRMLKNGSNVIGVIPKSLVDTEKAHDGLTELHVVNSMYERKILMCK
                     LSDGFILLPGGSGSLDEFFEMFTLVQLGYHKKPCCVLNASGYYDYLLQFLDYAVNQGF
                     LHSKDRNTLIVNQYPDTLIENLFEVINNRK"
     gene            779..1519
                     /locus_tag="ACF2SP_002788"
     CDS             779..1519
                     /locus_tag="ACF2SP_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFD2360555.1"
                     /translation="MPKKLLIAAFLCNIFCNPSHLAYASSTEIPILGKTMTQAKQQQM
                     TPRQALQRLKDGNQRFLSNKPLARDYLKQAKQSAYGQYPFAVILNCMDSRSVPEFFFD
                     QGLADLFTLRVAGNVLNDDILGSMEFATKVVGARLVVVLAHTSCGAVAGACKDVKLGH
                     LTDVINKIQPVVKPSMESTGIDNCSDPKLIDDMAKANALHVVKNILEQSPILNELVKS
                     KQIGIVAGIHDIKTGKVTFFEEKRSVPE"
     gene            1616..2836
                     /locus_tag="ACF2SP_002789"
     CDS             1616..2836
                     /locus_tag="ACF2SP_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFD2360556.1"
                     /translation="MPKSHNSIIFLGLLSAFSLLTFDLYQPSLPFITNYFNITPSMGQ
                     LTLSIYLLVYGITHLLWGPLIDHFGRRRLLPGNLLLALIGSLMCAFAPNITMLICGRA
                     LQGFALCCANLIAYSAARDFEDTIVRAKVLSYISMTVSVSPIIAPVIGALIFHHFGWQ
                     ANFIVMAIVACILLIQTKLSLLESPFWTPPQERFSVKKILREYKSILNIPSLWCASFI
                     LMFGISAVMLTVINSSYLIIEVLNYSPLAYGLIFILNGLNIIFGNYLGIWLRDRLPII
                     TTIYLGNWFIVLGGTAMLVTSKLFGFSLLALSFSLIANLGISVSAAPTISIALTDFKQ
                     NAGIVMAFMNTIRLIGPSLLTILTGYLLTRNLDALPLGLIGSGIGALFFSWYFSRLTT
                     ESRNSDMDSEEALT"
     gene            complement(2833..4098)
                     /locus_tag="ACF2SP_002790"
     CDS             complement(2833..4098)
                     /locus_tag="ACF2SP_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360557.1"
                     /translation="MKQPSRKQGYWEELCQWGNDKDHGNGLIAAVAKITVSDLDLERL
                     TNALRLLQIQQPLLRAKVIKSNQRCQFNIEEGEAVYLPFQIIKRTSNSVWKEVIELEL
                     NRPPRNQDYQWRTVLIYDQYGNSHEILLIAGHNIGDGVSVAYFFDTLFRIYQKSDSNP
                     SFPLYPPVEELVQHKKGQKSSLPPAFLPTPIYYQNYVPPGERITKNHYYPVTVKQTGA
                     LLAFSKNHNFSLNALINALSLMTLTKVLDQPLDTTLHTPVNLRKWCMPVIPEDYFGCY
                     VSVVTTYHKQVSIESDIIELARDYDHQLKQERLPGDAIFPVHHSKQDIIDALSSWEGH
                     RDRFSMGVTVTNIGLLPFNTQYGHLKWEKSYFCTSRQNADIPVLLNVLTLNDQLMFCF
                     TFTEPMCGSVFQENYVKNFISNMGKLTDC"
     gene            4406..>5158
                     /gene="ceg32"
                     /locus_tag="ACF2SP_002791"
     CDS             4406..>5158
                     /gene="ceg32"
                     /locus_tag="ACF2SP_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HFD2360558.1"
                     /translation="MTKIYLLTAPFDEEAKTGDGQYAASLELGFAENYEHIPCKWLKK
                     DKGDYSIPFPTGTTSIPEETIPSAIVLQPVANENTIISGYKLKDAVSSPEKAQEVNNK
                     TASPRTPKIIVKHDNSLQSLTIMDIYSQKPTQFDESKVDEIINSLEAKKVNLEKAIED
                     NNAELGKIKKQKSKLAYLTRLYKENKENIQDYCTLNEYIEAHLFNPKFLSRHEKALNN
                     FKALKSQFTGPVNLKELEKLTDKLTGIKEYSYD"
BASE COUNT         1614 a          990 c          930 g         1624 t
ORIGIN      
        1 aaatgctaaa aatcaatgtc tttacgcagg cgaataagaa tggtttgcgt tgaggcaatt
       61 aaattaaatc ctataaattt taatagtgaa aacattaact cattaaaaat aaaccgtatt
      121 tcgtcatttc atgttataaa taaaacattg catctggaat tttaaaatgc tccataatac
      181 caaaataaac aaactcgctg tcttttgtgg atctaacact ggttattctg aagtctataa
      241 acaatcagct gaaaacatgg ccgatgtcct aagcagtaat ggtattaccc tggtttatgg
      301 tggctcaaaa agcggtttga tgggaataat agccaatcgt atgttaaaaa atggctctaa
      361 tgtcattggt gttataccaa aatctctggt tgacacagaa aaagcgcatg atgggttgac
      421 ggaattacat gttgttaact ctatgtatga aagaaaaata ttgatgtgta aattgtcgga
      481 cgggttcatt ctgctgccag gtgggtcagg ttctctggat gagttttttg aaatgtttac
      541 cctggtacaa ttgggttacc ataaaaaacc ttgctgtgta ctcaatgcct ctggttacta
      601 tgattacctt ttgcaatttt tagactatgc tgttaatcaa ggttttttac actcaaagga
      661 tcgaaacacg ctcattgtca atcaatatcc agatacactc attgagaatt tatttgaagt
      721 aatcaataat cgcaaataac agtttaggaa aattaataac ataacaagga gttaaccaat
      781 gcccaaaaaa cttctcatcg ctgctttctt atgcaatatt ttttgtaacc ccagccatct
      841 ggcctatgcc tcatcaacag aaatacccat acttgggaaa accatgaccc aggctaaaca
      901 acaacaaatg actcccaggc aggcattgca acgtttaaaa gacggcaatc aacgcttcct
      961 aagtaataaa cctttagcac gagattatct aaaacaagcc aagcaatcag cttatggcca
     1021 atatcctttt gcggtcatct tgaattgtat ggactcgcgc agtgttcctg aattcttttt
     1081 tgatcaagga ttggcagacc tgtttacctt acgtgttgcc ggcaatgttc tcaacgatga
     1141 cattttagga agtatggaat ttgcaacaaa agtagtggga gcacgtttgg tagttgtcct
     1201 ggctcacacc tcctgtggtg ctgttgcagg ggcgtgcaaa gatgttaaat tagggcactt
     1261 gactgatgtt ataaataaaa tccagcctgt tgtaaaaccc agcatggaaa gcacaggtat
     1321 tgataattgc tctgatccca aactgattga tgacatggct aaagccaatg ccttgcatgt
     1381 tgtaaaaaat atactagagc aaagccctat tttaaatgaa ttagttaaga gtaaacaaat
     1441 tgggattgta gcgggcattc atgatattaa aacgggtaag gtgacttttt ttgaagaaaa
     1501 gcgatctgtc ccagagtaaa gcaagttggc aattgccaac ttgctttaat atttcctttc
     1561 aaatgcaggc tgaatttggt tacaatcgca taatttttgg tgaatgattt tttatatgcc
     1621 taaatcacat aattccatta tatttcttgg tttgctttct gcgttttctt tgcttacatt
     1681 tgatctttat caaccatcac tgcctttcat aacgaattat tttaatatta ccccaagtat
     1741 ggggcaatta accctaagta tttatttgct agtttatggc atcactcatc ttctatgggg
     1801 accacttata gaccattttg ggcgccggcg cctacttcct ggaaaccttt tattagcctt
     1861 aattggcagt ttaatgtgtg cttttgcgcc gaatatcacc atgcttattt gcggtcgtgc
     1921 cctacaagga ttcgccttat gctgcgcaaa tctcattgcc tattccgcag cacgtgattt
     1981 tgaagacacg attgttcgcg ccaaagtgct gtcctatatt tccatgaccg tttcagtttc
     2041 accgattata gccccggtaa ttggtgctct tatctttcat cattttggct ggcaagccaa
     2101 ttttatagtc atggcaatcg ttgcctgtat tctattgata caaaccaaac tctccttgtt
     2161 agagtcccct ttctggacgc ccccccagga gaggttctcg gtaaaaaaaa tactccggga
     2221 atataagtcc atactcaata taccctctct atggtgtgca tcatttattt taatgtttgg
     2281 catttctgct gtcatgctca ctgtaattaa ttcctcttac cttattatcg aggtactgaa
     2341 ttactctcca cttgcctatg gacttatttt tattctgaat ggtttaaata ttatttttgg
     2401 aaactatctg ggcatttggc ttcgtgaccg attacccatt ataaccacca tctatttggg
     2461 gaactggttc attgtccttg gtggtacagc gatgttggtt acttccaaat tgtttgggtt
     2521 tagtctgtta gcattatcat tttcattaat tgccaacttg ggaattagtg tcagtgccgc
     2581 tccaactatc tccatcgccc taacggattt taagcaaaat gcaggaatag ttatggcttt
     2641 tatgaatacc atacgtttaa tcggcccctc tcttttaact attctaaccg gctatttgtt
     2701 gacaaggaat cttgacgctc ttccactggg attaataggc tcaggaattg gagccctctt
     2761 tttctcatgg tattttagcc gattgaccac tgaatcgaga aactcggaca tggatagcga
     2821 agaagcattg acctaacagt ctgttaactt ccccatattg gatataaaat tttttacata
     2881 attctcctga aacacagaac cacacattgg ttctgtgaag gtaaaacaaa acatgagttg
     2941 atcattaagg gtaagaacat tgagcaaaac aggtatgtct gcattttgcc gggatgtaca
     3001 aaaatagctt ttttcccatt tcaaatgacc atattgagta ttaaaaggta acaaaccaat
     3061 attggttacg gtcactccca ttgaaaagcg atctctatga ccttcccatg atgacagagc
     3121 atcaataata tcctgcttac tgtgatgaac cgggaatata gcgtcaccag gcaatcgctc
     3181 ttgttttaac tgatgatcat aatccctggc aagctctata atatctgatt ctatactgac
     3241 ttgtttatga tacgtagtca ccacagacac atagcagcca aaatagtcct ccggaatcac
     3301 aggcatacac cacttcctta aattaaccgg ggtgtgtagt gttgtatcta aaggctgatc
     3361 gagaactttt gttaatgtca ttagggacag cgcattaatt aatgcattca gagaaaagtt
     3421 gtgattttta ctaaaggcca gaagcgctcc agtttgtttc actgtaacag gataataatg
     3481 attttttgtt attcgctcgc caggagggac ataattttgg taataaatcg gagtaggcaa
     3541 aaatgcagga ggcagagaag atttttgccc ttttttgtgt tgcaccagtt cctcaacagg
     3601 gggatagaga gggaaggatg gatttgaatc acttttctgg taaattctaa agagagtatc
     3661 gaaaaaatag gctaccgaga cgccatctcc aatattatgt cctgcgatta acaggatttc
     3721 atgagaatta ccgtattgat cataaataag aacggttcgc cattgataat cttgattacg
     3781 gggaggtcga tttaattcca attcaatcac ctctttccag acagagtttg aagtcctttt
     3841 tatgatttga aatggtaaat atacagcttc accttcctct atattaaatt gacaccgttg
     3901 attgcttttg atcacttttg ccctaagcaa tggctgttgg atttgtaata acctgagcgc
     3961 gttggttaaa cgctccaagt ccaaatcaga aacagtgatt ttcgcaacag cagcaattaa
     4021 accatttcca tgatctttat cgtttcccca ttgacataac tcttcccaat agccttgttt
     4081 tctggatggc tgtttcatag attttctccc gaagccatgg tcatgattct ggaaagttcc
     4141 ttttacagag agcaatttcc atactgcagg acatgcacct gcttaattac ttttgatgga
     4201 cctaactcat attaatgctc taaattataa ataattgctt ttttcaagtt ctatttatgc
     4261 aattaagtca aaaaacctca taattttatc aacatattat catcttgcta ttcctgatta
     4321 tattttgcaa ttatacctct taatgttcat ttaataatct taatgtaaga tattgaccat
     4381 gacaaaaatt ttactaaagt aatttatgac taaaatatat ttattaacag caccttttga
     4441 tgaagaagca aaaactggtg atggacaata cgcggcctct ttagaattgg gttttgctga
     4501 aaattacgaa catataccct gtaaatggtt aaaaaaagac aaaggtgatt acagtatccc
     4561 attcccaaca ggaaccactt ccataccgga agaaacgata ccaagcgcga tagtattaca
     4621 gccagtcgct aacgaaaata caatcataag cggttataaa ttaaaagatg ccgtttcatc
     4681 tccagaaaaa gcacaggaag taaataacaa aacagctagc cccagaacgc caaaaattat
     4741 tgtaaagcat gataacagcc tgcaatcact tacaataatg gatatttact ctcaaaaacc
     4801 tacccaattt gatgagtcaa aagttgatga aataattaac tctttagaag ccaaaaaagt
     4861 aaacctggaa aaagctatag aagataataa cgctgagctg ggtaaaatta agaaacaaaa
     4921 atccaaatta gcgtatttga ctagattata taaggaaaac aaagagaata ttcaagatta
     4981 ttgcacctta aatgaatata ttgaggccca tttatttaac cctaaatttt taagcaggca
     5041 tgagaaagca ctcaataatt ttaaggcact taaaagccaa tttactggcc ctgtcaattt
     5101 aaaagaactg gaaaaattaa cagataaact gactggaatt aaagagtaca gctatgat
//
LOCUS       DAXLDT010000199         5154 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXLDT010000199 DAXLDT010000000
VERSION     DAXLDT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.200"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1134
                     /locus_tag="ACF2SP_002792"
     CDS             <1..1134
                     /locus_tag="ACF2SP_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HFD2360559.1"
                     /translation="YISKNKEIAINFGKTEFRDFVLSNLGISSHLGTCPTKNETGGDT
                     FYFHQENLSLNGPALSVNTKITKEIEKNDYLLSANGTKAIDFVWSNFLKHPYNPKLKQ
                     PDANVKAAWEKHTDWEHWSNMGQDWSLNTPSGLVPRPNHGIAHTLRVAQLVPVIAEFL
                     KAYSGDPKFQKLTQKEIQKAQYMMLFSVIGRENDMSWTDANHYQQAFKEAYNGQHSKH
                     IYATFKENAQKGFLNHVVTNKSSLIPSLFSDESELQYWAEALDTGKPGISSASGILMA
                     LAHDLDLMRCYDKGKFNSLKMKDLVTRLGGNEEAARKLADYAHDLIVATGDRCMGYGV
                     SQDYNFSLFGQCSLDPNECLKKLQSVPKPETTPTASLTNHHGI"
     gene            1354..4035
                     /gene="pepN"
                     /locus_tag="ACF2SP_002793"
     CDS             1354..4035
                     /gene="pepN"
                     /locus_tag="ACF2SP_002793"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFD2360560.1"
                     /translation="MKIDQSKVKKLNDYKVLDYFVIHVDLQIDLGKKPVESKARLTVV
                     PNPSVDSHSNDLILDGENMTLVSLQMNDNLLKEHEYELTKDALIIKNIPQNTPFTIET
                     TSLLGENTDLFGLYETEGVALVKAESEGLRRVLYLPDRPDNLATYKTTIIANQEDYPV
                     LLSNGVLIEKKELPLGLHSATWLDDVPKPSYLFALVAGNLQRSVTYYQTKSGRELPIE
                     FYVPPSATAKCDFAKEVLKEAMAWDERTFNLECALRQHMVAGVDKYASGASEPTGLNL
                     FNTENLFATPETKTDLGILRVLEVVAHEFFHYWSGDRVTIRDWFNLPLKEGLTTFRAA
                     MFREELFGTDLIRLLDGKNLDERAPRQSAYTAVRSLYTAAAYEKSADIFRMMMLFIGK
                     KTFIQGVAKFFKDNDGSAVTLEDFIDSLSNSSGKDLRSFLSWFTESGIPELIVTDEFN
                     PDAKQYLLKIKIANGKNRPVPFLMGLLDSSGKEIVADTLLMVDQEEIEFQFENIQTRP
                     IPSLLRSFSAPVQMNYEYSYQDLLLLMQFDTNLYNRCEAAKRLISVLINDFCIGKKIE
                     LSPQFFAVYKALLSDNSLNEWMLAELITLPSLEELIENQDKPDFEKLNEGRQLIQNAL
                     ANELKTDFYNLLFRIQISGDDDKQKLKGFDLKQAGLRRLKSVCFSYLLNVDFEKTKEK
                     LILQFEDALGKNMTETALALSMLCEINCEEADVALEDYYHYWKNDPGAVNNWFSIQAL
                     AHSPDVIERVKKLMRHSDFDLSNPNKVYALIGSFIKNPFGFHSVTGEGYQLVADAILD
                     LDEINPTLAANLTEKFTYWDKYDVNRQAMMISTLKIIYSNATSSDVRTMAKKGLDKVK
                     EDLPLPIHLTFHGGSTMQDRTTQLIADGNKENAYQLY"
     gene            complement(4170..5093)
                     /locus_tag="ACF2SP_002794"
     CDS             complement(4170..5093)
                     /locus_tag="ACF2SP_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2360561.1"
                     /translation="MSSKNTHSTLLNKQEINWILEQRITRLTDNKEVLIYPMGAERYL
                     SSALSGHEEERYNLHSEIKEKNVLIIPGYGNSAFLFAQAGAKSILVYDKDPVTIAWVK
                     AFKKYYHYREYNNSGKPYPSIGELLTALTKWYPPLLKLPSGVIKNSVFWIVNPKLLRK
                     SYIYYMLKLTRQAVQLRVQNEFEFDHHIEFYAGELKHLIKNHPSKTFDTVFVPYLLGV
                     QNGIEKEKDIVSFIKQLIELVPYGHILVTPSKSTKEFHLVGQSYFCTTAYSNIQSIPD
                     LKPYIIAEDSIWFKTQGLAVFGIKPGAENRL"
BASE COUNT         1676 a          822 c          994 g         1662 t
ORIGIN      
        1 tatatttcaa agaataagga aattgcaatt aattttggaa agacagagtt tagagacttt
       61 gtgttgtcta atcttggaat aagctctcat ttaggaacct gtccaacaaa aaatgaaaca
      121 ggtggtgata ctttctattt tcatcaggag aatctttctt taaacggccc tgcattatca
      181 gtaaatacca aaataactaa agaaatagag aaaaatgatt atctactgtc tgctaatggg
      241 actaaagcca tcgattttgt ttggagtaat tttttaaaac atccttataa tcctaaactg
      301 aagcagccag atgctaatgt aaaagcagct tgggaaaagc atactgattg ggagcattgg
      361 tccaatatgg gacaggattg gtcattaaat actccctcag gtttggttcc caggccaaat
      421 catggaattg cccatacgtt acgagttgca cagcttgtac cagtcattgc tgaatttttg
      481 aaagcatatt caggtgatcc aaaattccaa aaattaacgc aaaaagaaat acaaaaagca
      541 caatacatga tgttattttc agtaattggg cgtgaaaacg atatgagttg gacagatgcc
      601 aatcattatc agcaggcatt taaggaagca tacaatggac agcattccaa gcacatctat
      661 gctactttca aagaaaatgc tcaaaaaggt tttttaaatc atgtggttac caataaatcg
      721 tcattaattc cttcgctatt ttccgatgaa agtgaacttc aatattgggc tgaagctctg
      781 gatacgggta aaccaggaat ttcatcagcc tctggtatac ttatggctct ggctcatgat
      841 ctggacttaa tgagatgtta tgataaggga aaatttaata gtcttaagat gaaagattta
      901 gttaccaggc ttggaggaaa tgaagaggca gcaagaaaac tagctgatta tgctcatgat
      961 ctgattgttg cgacaggaga tcgatgcatg ggttatgggg tgtctcagga ttataatttc
     1021 tctctttttg gtcaatgtag ccttgatccc aatgagtgtc taaagaaact tcagagtgtc
     1081 cctaaaccag aaacgactcc aacggcttct ttgaccaatc atcatggtat ttgagtttta
     1141 actctctttt taaatcatac tgctctttaa tcaagtatcc tttgagtgca aaggatacta
     1201 tatatcacca taacaaaaac actaacttga ttgaaataac tatcttgatc ttatatcgtg
     1261 atttcactga gctgaaaaca gataaatggt ttgtaatcag ttgcaatgag gaatattaat
     1321 tcttagtttt ttgtggtaaa tcagggagtg ttcatgaaaa tagatcaatc taaagtaaag
     1381 aaattaaatg attataaggt tctcgattat tttgtaatcc atgttgattt gcaaatcgat
     1441 ctcggcaaaa agccagtaga atctaaagcc cggcttacag tagtgcccaa cccaagtgtt
     1501 gactcacatt caaatgattt gatattggat ggtgagaata tgacattagt gtcgttacaa
     1561 atgaatgata acttattgaa agaacatgaa tatgagttaa cgaaagatgc tttaataatc
     1621 aaaaatatac ctcaaaatac tccctttact atagaaacga cctctctgct cggtgaaaat
     1681 accgatttgt ttggattata tgaaacagag ggagttgcct tggttaaggc agagtctgaa
     1741 ggattgcgcc gagtattata tttgccggac agacctgata atctcgcgac ttataaaact
     1801 actatcatcg cgaatcagga agactatcct gttttacttt caaatggtgt gctaattgag
     1861 aaaaaggaac ttcctttggg cctgcattcc gcaacctggc tggatgatgt cccaaaacca
     1921 agttatttat ttgctctggt agcaggtaat ttacagcgtt cagtcactta ttatcaaaca
     1981 aaatctggcc gtgaattacc catcgaattc tatgttccac catcagctac agcaaaatgt
     2041 gattttgcaa aggaagtatt gaaagaggca atggcatggg atgaacgtac ttttaattta
     2101 gaatgtgcct tgagacaaca tatggtagcc ggagttgata aatacgcgtc aggtgcttcc
     2161 gagccaactg gcttaaattt atttaataca gaaaatttat ttgcaactcc tgaaactaaa
     2221 accgatttag gtattttgcg cgtgttggaa gtggttgccc atgagttttt tcattattgg
     2281 tcaggagatc gggtcacaat aagagattgg ttcaatttgc cattaaagga aggtcttact
     2341 acgtttagag ctgctatgtt cagagaggag ctgtttggta cggatttgat aagattactt
     2401 gatggcaaga atttggatga aagagcacca cgacaaagtg cctatacagc agttagaagc
     2461 ctatatactg ctgcagccta tgaaaaaagt gctgatattt ttcgtatgat gatgttgttt
     2521 ataggtaaaa aaacatttat tcaaggggta gccaagtttt ttaaggataa tgatggaagc
     2581 gcagttactc tggaggattt tattgattca cttagcaatt catctggtaa agatcttcgt
     2641 tcctttttat cctggtttac tgaatctgga attcctgaat tgatagtaac ggatgaattt
     2701 aatccagatg caaagcaata tcttttaaaa attaaaatag ccaacgggaa aaaccgcccc
     2761 gttcctttcc tgatggggtt gcttgatagc tcaggaaaag aaattgtagc tgatacatta
     2821 ttaatggttg atcaagagga aatcgagttt caatttgaaa atatccagac ccgccctatt
     2881 ccatcgttat tacgtagttt ttctgcaccg gtacaaatga actatgaata ttcttatcag
     2941 gatttattat tattgatgca attcgacact aatttgtata atcgttgtga ggcagcaaaa
     3001 cgacttattt cagttttaat taacgatttt tgtataggaa aaaaaataga attatctcct
     3061 cagttcttcg ctgtttacaa agcattactt tctgacaact ctttaaatga atggatgcta
     3121 gctgaattaa ttaccttacc gtctttagaa gagttgattg aaaatcagga taagcctgat
     3181 tttgaaaaat taaatgaggg gcgccaatta atacaaaatg ctcttgcaaa tgaattgaaa
     3241 acggattttt ataatctcct ttttagaata caaatatctg gggatgatga taaacaaaaa
     3301 ttaaaaggtt ttgatttgaa gcaagcggga ttgcggcgtt taaaatccgt atgtttttct
     3361 tatttgctaa atgttgattt tgaaaaaaca aaggagaaac tgattcttca atttgaggat
     3421 gctttaggca aaaatatgac agagactgct ttggctttaa gcatgttatg cgaaataaat
     3481 tgtgaggaag cggatgttgc attagaggat tattatcatt attggaaaaa tgatccggga
     3541 gctgtaaata actggttcag tattcaagca ttggcacact cccctgatgt gatagagcgg
     3601 gtaaaaaaat taatgcgtca tagtgatttt gatttgtcta atcctaacaa ggtatatgct
     3661 ctgataggat catttataaa aaacccattt ggatttcatt ctgttacagg tgaaggttac
     3721 caactggttg ctgatgccat tttggacttg gatgaaatta atccaacttt ggctgccaat
     3781 ctaactgaaa aatttactta ttgggataaa tacgatgtaa ataggcaagc tatgatgatt
     3841 agcactttaa aaataatcta ctctaatgct acttcatctg atgtgagaac aatggcaaaa
     3901 aagggattgg ataaggtaaa agaagatctc ccattaccaa tccatttgac ttttcatggg
     3961 ggatcaacca tgcaggatag aacaactcaa ttaattgctg atggaaataa ggaaaatgct
     4021 tatcaattgt attgatttct tatttcataa cctgatttag aaaagcgcga gatattgata
     4081 ttgcctaaaa cttggggggt gaatactccc cttccttggt aggtaatgag gaagcacgtt
     4141 ttctaatgca ataaatcata gccttatttt cacaaacgat tctctgcacc tggttttata
     4201 ccaaatactg caagtccttg agttttaaac caaatactgt cttctgcaat gatataaggt
     4261 tttaaatcag gaatggactg tatattagaa taagctgttg tacaaaagta actttgccca
     4321 accagatgga attctttagt gctttttgaa ggagtcacta aaatatgacc atagggtaca
     4381 agctctatta attgcttaat aaaactaaca atatctttct ctttttctat tccattttgt
     4441 acacctaaga ggtaaggaac aaatactgta tcaaatgttt ttgacggatg attttttatc
     4501 agatgtttta attcacctgc ataaaactct atatgatgat cgaactcaaa ttcattttga
     4561 actcttaatt gtacagcttg cctggtgagt ttcagcatat aataaatata tgattttctt
     4621 agtaatttag ggttaactat ccaaaataca gagtttttaa tcactcctga aggtagttta
     4681 agtagaggag gataccattt ggtgagggct gtcagaagtt cacctattga aggatatggt
     4741 tttccactat tattgtattc tctgtaatgg taatattttt taaatgcttt tacccatgca
     4801 atagtcacgg gatctttgtc ataaacaaga atagatttgg cccctgcttg agcaaataaa
     4861 aaagcgctat taccatatcc agggataatt aggacatttt tttctttgat ttcactgtgt
     4921 aaattatacc tttcttcttc atgaccagat aaagcagatg acaaatagcg ttctgcaccc
     4981 attggataaa ttaaaacttc tttattgtca gtcagtcttg taatacgttg ctctaaaatc
     5041 cagtttattt cttgcttgtt gagtaaagtg ctatgggtgt ttttacttga catgaatagg
     5101 cctttcataa aaggattaga actggctggt gctaaatgtg attattaatt tagt
//
LOCUS       DAXLDT010000200         5040 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXLDT010000200 DAXLDT010000000
VERSION     DAXLDT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.201"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            150..410
                     /locus_tag="ACF2SP_002795"
     CDS             150..410
                     /locus_tag="ACF2SP_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFD2360562.1"
                     /translation="MNKAATINARIEPALKIQAEEILHKVGLSAAEAIRLFYSQVCLQ
                     NGLPFDVKIPNKKTLDAIEELESGKGERFKTMKDVWNSIDNA"
     gene            403..657
                     /locus_tag="ACF2SP_002796"
     CDS             403..657
                     /locus_tag="ACF2SP_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HFD2360563.1"
                     /translation="MLELELATQFKRDLKKIVKQRKNKDLLDSILDPKHKDHNLSGDW
                     HGYRECHITPDWLLIYKIIKDDCVQLLRLSRTGSHSDLFK"
     gene            719..1099
                     /locus_tag="ACF2SP_002797"
     CDS             719..1099
                     /locus_tag="ACF2SP_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HFD2360564.1"
                     /translation="MELLDKISALEEEASQFGFKWQSADQIMNQIHSECDEIKEHLNH
                     DSSKENQTALQEEIGDLLHAVFSLCIFCKLSPRVTLGQTLTKFERRLRAVKLIAEERE
                     LINLEGLSLNELMHIWDRAKELVG"
     gene            2088..2666
                     /locus_tag="ACF2SP_002798"
     CDS             2088..2666
                     /locus_tag="ACF2SP_002798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360565.1"
                     /translation="MTAKTLKGSIEEQSQVLENTQKELEQVVLAYKDNQNQLSEKIKE
                     LDEVKVKMSKEVDQAQTLSLVLRSTVEALSKTVIADEEQRTSFQLRLNEFLTNKEKSF
                     DQVAERICEAERKLSVVTKQLEESNQRYRKLLDRQEQQIIRLEQIDVVQPEEVQESFD
                     GVKPSVNGFYAIKKEPPFFPASTHRTPMVAVV"
     gene            2714..2929
                     /locus_tag="ACF2SP_002799"
     CDS             2714..2929
                     /locus_tag="ACF2SP_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HFD2360566.1"
                     /translation="MEIRTVAFESELIITLENNQKVVITPFKTQEPGNFKLGIDAPKQ
                     VTVNRQEIYLRKQEQLNRNKKSTSTES"
     gene            complement(3029..>5040)
                     /locus_tag="ACF2SP_002800"
     CDS             complement(3029..>5040)
                     /locus_tag="ACF2SP_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2360567.1"
                     /translation="KNLIDNLPGDIYWKDTEGIWSGVNKRGAQSLLQIGFIRDRSDVI
                     GKSDYQLFSKETADEYRKNDLEVMSLKTEMTREEINQLPNGEKIIQLSSKRPLWDKEG
                     NVVGVVGNTIDISYLKKIEADLKTAMEKAEAASKAKTEFLENMRHDIRTPLTGIVGFA
                     DLLKMESDNPHIKEYSENLVASSHALLDLLDEVLEAIRVSSGEIPKLKKKFSLEKTLS
                     QVIELNRARSLEKKLSLTLNLDKSIPKYVIGDKVRLHRVALELVANALNFTDNGFVTL
                     NALLAKKDEGKLVLKLIVEDSGIGIPKDKQQEIYVQFKRLTPSYQGIYKGAGLGLSVV
                     KQFVDEMGGEIYVDSEVRCGTTFTCLIPLQEPLLDDATGIDSEMEHIVDTPLETTYAQ
                     EIKPSVESKQCHAFRVLVVEDNPIAQTVAKAILAQLHCDTDIADTGKKAVELWRNGQY
                     NLIFMDIGLPDMDGYEVTHQIRLHEITQKTHSPIIALTAHAGDENKQRCIEAGMNAVL
                     TKPLTAKSCTDILDAFIPGYHQQNNTPKEIAYRSELPQQDESLFELTPYSILDVEEGI
                     KTTGTIEALAEMLHFMVHDSLLPDVKLMKQAHDAMDWDKIQQLAHKIKGGAVYVGTVK
                     IKMACQYLERYWKSGQRELMEPLYQQTIKVIDESIQEINLWLKSH"
BASE COUNT         1560 a          897 c          980 g         1603 t
ORIGIN      
        1 ttaaagtggg gccagaatcg atgtttttat gaggagccat aggccaactt gattatcggt
       61 aattagacgc ctctttgtgt aaaacaaaca ttgaaaatgt aacgcatttg ttatacaatt
      121 taattatgaa aagtattgga gtgaatatca tgaataaggc ggcgacaatt aatgctcgta
      181 ttgagcctgc tcttaaaata caggcggaag agatattaca taaagtaggt ttatctgctg
      241 ctgaggctat tcggcttttc tatagtcaag tatgtcttca aaatggtctt ccatttgatg
      301 taaaaatacc gaacaaaaag acgcttgatg caattgaaga gcttgagtca ggtaagggcg
      361 aacgatttaa aacaatgaag gatgtttgga attctataga taatgcttga attagaactg
      421 gctactcaat ttaagcgtga tcttaaaaag atagtaaagc agaggaaaaa taaagatctt
      481 ttagattcta ttttagatcc caagcataaa gatcataatc tttcaggaga ttggcatggt
      541 tatcgtgagt gtcatataac ccctgattgg cttttaattt ataaaatcat taaagatgat
      601 tgtgtgcagt tgttaagatt atcaagaaca ggatctcatt ctgatttgtt taagtaagta
      661 tggcatcatt ctaatgcgta cttctaagaa tattagtcaa atctctttta ggttgaacat
      721 ggaactttta gataaaatta gcgctctgga agaagaagcc tcccaatttg ggttcaagtg
      781 gcaaagtgca gaccaaatca tgaatcaaat tcacagtgag tgtgatgaaa tcaaggagca
      841 cttgaatcat gatagttcta aagaaaatca aactgctttg caagaagaga ttggtgattt
      901 actccacgca gtgttttcgc tgtgtatttt ttgtaagtta agcccaagag taacacttgg
      961 acaaacgctg actaaatttg aaagacgatt acgagcggtt aaactgattg ctgaggaacg
     1021 tgaattgatt aaccttgagg gtctatccct taatgagttg atgcatattt gggatagggc
     1081 taaagagtta gttgggtaaa gagctgtaat ctcattatta aaagttaagc tgaattctgg
     1141 gtcttttttg ttgtgcgtgg aattaaattc tgtagtgagc taaccaaggg acgtcctgta
     1201 tacatagaag tcatagttgt gcctgtgcgc agctgtgatt gccattgaat tgagatcatc
     1261 cttgatctcg ctagtgtttc aacatcctgt caacaatcca gctgcgccaa acaattacat
     1321 tgtagcttta tattaataaa ataaattgat ttgtttgctt atttttttgt aagatatttc
     1381 tcaaattaaa tactgatata ccttggcgtt cgtttcaatc gaccatgtat gtttaatgat
     1441 ctatttgatc caattaacgt acatacactt caatcctgct ttttattttt tatctacttt
     1501 tatctaatct tgataagtat atgacaaaca acaatataat tgggtagccg ttaaaattac
     1561 ataaattaag acactttttg tttacttggc ttaagaaaaa aaaatattga tgatgctttt
     1621 atccaacctt actccgaaca tgcgaaaaga gattcgttag aaatttattc aaaattaagc
     1681 ttagctgatg cacaagataa atataatgat gttattggag acttcccagt ctgatatttt
     1741 aaatttttgc atcagggcgc aagttctgga gcgctacgcc tgttactgga attatttatg
     1801 cggtaagtca catccttctt gaaaatcatc aaaaccaaaa cacaaacaac accaaacagt
     1861 taaacgcagg aatttcaagt cttgttgacc tgctggatac ggttatttca actttagatc
     1921 tcttacgcga acaattagct cttgaaatcg atgcgtttca gaaagaaaat gcgcgcctaa
     1981 cccaaaatgt agatcaattt tgtgagcaaa ttaaaacatt aaaatcagaa atctgtaagc
     2041 ttaatgacac cgaaaaggca ctgcgtgcaa ctcagattga actcgaattg actgccaaaa
     2101 cgcttaaagg ctctattgag gaacagtctc aagttttaga aaacactcaa aaggaattag
     2161 agcaggttgt cctggcatat aaagacaatc agaatcagtt atctgaaaaa attaaagagc
     2221 ttgatgaagt taaagtaaaa atgagtaaag aggttgatca ggcacaaaca ctgtcccttg
     2281 ttctaagaag tacagtagaa gccctatcaa aaaccgtcat agcagatgaa gagcaacgta
     2341 cctcatttca attaagactc aatgaattcc tgactaataa agaaaagagc tttgatcagg
     2401 tagcggagcg aatatgcgaa gccgaacgca aactctctgt tgttacaaaa caattagaag
     2461 aaagtaatca gagatatcgc aagcttttag accggcagga acaacagatt attcgtctgg
     2521 aacaaattga tgtggttcaa cccgaagagg tgcaggaatc gtttgatggt gtaaagccaa
     2581 gcgttaatgg gttttatgct ataaaaaaag aacctccatt ttttcctgct tctacccata
     2641 ggactccgat ggttgctgta gtataacagc tgctttattt gcaattattt tgaactaaaa
     2701 cgagtaaatc aatatggaaa taagaacagt agcctttgaa tctgagttaa ttattacgtt
     2761 ggagaataat caaaaggtgg tgattacccc ttttaaaacc caggaacctg gaaattttaa
     2821 actgggaatt gatgcaccta aacaggtgac tgttaacagg caagaaatct atttgagaaa
     2881 acaagaacaa ttaaatagaa ataaaaaatc gacatcaacg gagtcttaag tgattttatt
     2941 ataatgaatg gggtaattaa aatttattat ggaaatatta acttttgagg tgctcataac
     3001 gaaggttgct aaactgtgta ttacttgact aatgggattt taaccaaaga ttaatttctt
     3061 gaatgctctc atcgatcact ttgattgttt gttgataaag tggttccatt aactcacgtt
     3121 gcccagattt ccaatatctc tccagatatt ggcaagccat tttgattttt actgtaccga
     3181 catagacggc accacccttg attttatggg ctaattgttg gattttatcc caatccattg
     3241 catcatgagc ttgtttcatt aatttaacat caggaagaag tgagtcatga accataaaat
     3301 gcagcatttc ggcgagtgcc tcaatggttc cggtagtttt aattccttct tcaacatcca
     3361 gaatagagta gggggtcaat tcaaacaaag attcatcctg ttggggtaat tcagaacgat
     3421 aggcgatttc tttaggtgta ttattctgtt gatgataacc cggaataaat gcatcaagta
     3481 tatcggtaca acttttcgca gttagtggct tggttaaaac ggcattcatt ccggcttcta
     3541 tgcagcgttg tttgttttca tcaccggcat gggctgtaag ggcaatgata gggctgtgtg
     3601 ttttttgggt tatttcgtgc agtctgattt gatgggtgac ttcataacca tccatatcgg
     3661 gtaatccgat gtccatgaaa atgagattat attggccatt tctccatagt tctaccgctt
     3721 ttttacccgt gtccgctatg tctgtatcac agtgcagttg cgcgagaatc gctttagcaa
     3781 ccgtttgggc aatggggtta tcttcaacga ccagaacacg aaatgcatgg cactgtttcg
     3841 attcaacgct tggtttaatt tcctgggcat aggttgtttc taagggtgtg tcaacaatgt
     3901 gttccatctc cgagtcaata cctgtggcat cgtcaagcaa cggttcttgt agaggaataa
     3961 ggcaagtaaa ggtagtgccg caccgtacct cactgtccac ataaatttca ccacccattt
     4021 catcaacaaa ctgtttgacc actgaaagac caagcccagc acctttataa atgccctgat
     4081 aggatggggt aagtcgttta aattgcacgt agatttcttg ttgtttgtct ttaggaatgc
     4141 ctatccctga atcttccact ataagtttta ataccaactt cccctcgtct tttttggcaa
     4201 gcagagcatt gagggtaaca aagccgttat cggtaaaatt cagtgcgttg gcaaccaact
     4261 caagagctac acggtgtagg cgaactttat caccaatcac atatttgggt atggacttat
     4321 ccagattcag agttaaggac agtttttttt ctaaggatcg tgcacgattg agttcaatta
     4381 cctgacttaa tgttttttcg agactgaatt ttttcttgag ttttggaatt tctcctgaac
     4441 ttactcgaat cgcttcaagc acttcatcta ataaatccaa tagagcatgg ctcgatgcta
     4501 ccaggttctc tgagtattct ttaatgtgag ggttatctga ttccattttc agtaaatcag
     4561 caaaacccac aataccagtt aatggcgtac ggatgtcatg gcgcatattc tcaaggaatt
     4621 cggttttggc cttgcttgcg gcttccgctt tctccatagc cgtttttaaa tcggcttcaa
     4681 ttttttttaa gtaagaaata tcaatggtat taccaacaac cccgactaca ttaccttctt
     4741 tatcccacaa tggtcttttg cttgagagtt gtattatttt ttccccatta ggcagttgat
     4801 taatttcttc gcgagtcatc tccgttttta agctcataac ctccaagtca ttttttctgt
     4861 attcatcagc ggtttccttg ctaaataatt gataatcaga ttttcctatg acatcagagc
     4921 gatcacgaat aaatcctatt tgtagtaggc tttgtgcacc tcgcttatta acccctgacc
     4981 aaattccctc tgtgtctttc caataaatat ctcctggaag attatcaatg aggtttttta
//
LOCUS       DAXLDT010000201         5024 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXLDT010000201 DAXLDT010000000
VERSION     DAXLDT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.202"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            189..464
                     /locus_tag="ACF2SP_002801"
     CDS             189..464
                     /locus_tag="ACF2SP_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HFD2360568.1"
                     /translation="MKQKKIYVGNLPFGTTEEALSIQFSQYGKIEEMFLIKDRLTGQM
                     KGFGFITFSAQQEAESALEMNGQPFDGRSLKVCMAQKKINSVSRSRW"
     gene            640..1317
                     /locus_tag="ACF2SP_002802"
     CDS             640..1317
                     /locus_tag="ACF2SP_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFD2360569.1"
                     /translation="MKAKGFFDEQSSLKKGKIGLNSVWMFSLGHIFLITLSNVLVQYP
                     FAMMGFHTTWGAFTYPAIFILTDLTTRIATANKARKIIMLSMTPGLILSYLVASYIEA
                     GNNLNENVLSVLHLMPLRIALACFIAYVVGQLLDVAVFQRYRNRPAWWLAPTLSAVAG
                     NFIDTALFFTIAFYHCSNPFLSQNWPEIAMVDVFFKIMISLIAFVPVYGLVLSILGIK
                     TTNKLMA"
     gene            1355..1993
                     /locus_tag="ACF2SP_002803"
     CDS             1355..1993
                     /locus_tag="ACF2SP_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFD2360570.1"
                     /translation="MLTGLRLLAKDLKILNGKYIQLEPITSQHREELRKAANHEQIWQ
                     YMPQKATGKLFNSWFDDCLEKMSIRDQITYAVRCKKEGDLKGATSFYDIQPENKRLAL
                     GYSWYIPKVWGTTANPESKLLMLHQAFEAWGINRVELGTDSRNIHSYRAIKKLGATEE
                     GVLRQHMILHNQVITDTIMFSILFSEWPAVKERLIQRLNYAATDKDLLAVSI"
     gene            2127..2654
                     /locus_tag="ACF2SP_002804"
     CDS             2127..2654
                     /locus_tag="ACF2SP_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360571.1"
                     /translation="MYNKMFKPLDTDPILYFKMYSNYTEGRVDDCCAFILMPSGLQRE
                     WVCLQSIQFAFNKRGDVLGINIIFSGNESNIHKKVRETMEGMLKLKLQYGRGEELFVF
                     DEEKKTFHMGIVPGKDTQAYLEGIIAFIKDSYRLQPDFAQDIKSQLLSKEYLAQEYSR
                     LRWKPPEKETVCVLM"
     gene            2809..4155
                     /locus_tag="ACF2SP_002805"
     CDS             2809..4155
                     /locus_tag="ACF2SP_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFD2360572.1"
                     /translation="MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQT
                     EQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHLAMANIESAQGQLQQVQLSWLPN
                     LTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
                     IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQR
                     IEAQIKVVQHNIVVTKNALHFLFNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRP
                     DIHEAEALLKAAHADVGAITANLLPGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLP
                     LFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALNDEKQQC
                     HLAEVRYRHGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVTLYQDLGGGYRGY"
     gene            4145..>5024
                     /locus_tag="ACF2SP_002806"
     CDS             4145..>5024
                     /locus_tag="ACF2SP_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD2360573.1"
                     /translation="MGIDQQKTVRTLRLSLTCIFLFLITWYYQVPESAWTLVTIWFVM
                     YEYSTVGGVLTKSFLRFAGTALSAIYGMIVVYFCANNPLINIMALVPGLFLYAYFFMG
                     GDKTYIGTIGAVTLTIVLLNYNDIDTAVLRVFNVIIGVIGSMFMIRFFYPQYARDKVL
                     EIQLNFITQLANLLESYLNPTQSLAAVQTKYLDYERKMLDGFTLYNRYIGEAKIETKK
                     APFFIPHSIAAMQHFRRLFRLFSVFVYYLSTEEIRSDPWVCDQLSKLLCNLQSLQRKL
                     LKQDAGPIGGDVVAAEP"
BASE COUNT         1489 a          905 c         1047 g         1583 t
ORIGIN      
        1 ctattataac agttatttgt aaatatgtct tgtttattat gcttaggttt ggattgttaa
       61 ttaacaggta acatgctaag cttctcagta gatttaattc tacgcacaat actcttttcg
      121 atcgtttgat tttttactct tcttgctgtc ctccagatca ttgttcgtta atctattcag
      181 gagcaatgat gaaacaaaaa aaaatatatg ttggtaattt accatttggt acgactgaag
      241 aagcattaag cattcaattt tctcaatatg gaaaaatcga ggaaatgttc ttaattaagg
      301 atcgattaac agggcaaatg aagggatttg gttttataac gttttctgct caacaggaag
      361 cggaatctgc gttagagatg aatggtcagc catttgatgg acgttcatta aaagtatgta
      421 tggcacaaaa aaaaatcaat tctgtttctc gtagccggtg gtaaaataga gtatttactt
      481 aattccgatg ttatcgtatc gagaactcat cactacaaat aaatttttaa tctacgttgg
      541 ctcaactttt ttagccccta gtgagccatt tattttcttg tgttttatct atcccatatg
      601 caaaaataac aaccatagta tttggtgata atagtcagca tgaaagcgaa aggttttttt
      661 gatgagcaat cttccctgaa gaagggtaaa atcgggttga attcagtatg gatgttctca
      721 cttggtcata tttttctaat aacgctcagt aatgttctgg ttcaatatcc atttgctatg
      781 atgggatttc acacgacctg gggggcgttc acttatccag caatttttat tttaactgat
      841 ttgacaacaa ggatagcaac cgctaataag gcgcggaaaa ttattatgct gagtatgacc
      901 ccaggattaa tattgtccta tcttgttgcg agctatattg aggcaggcaa taatttgaat
      961 gagaacgttc tatctgtttt acacctcatg ccattacgta tcgccctggc ttgttttata
     1021 gcctacgttg ttgggcaatt gctggatgtg gcagttttcc aacgctatag aaatcggcct
     1081 gcctggtggc tggccccaac tctatcggcg gttgcaggca atttcataga tacggcattg
     1141 ttttttacca tcgcgtttta tcactgctcc aatccatttt taagtcagaa ttggcctgaa
     1201 attgcgatgg tcgatgtctt ttttaaaatc atgattagtt taattgcttt tgttccggtt
     1261 tatggtctgg ttttaagtat tcttggtata aaaactacca ataaattaat ggcataaagg
     1321 tatcttgaca ccattttcaa aataaggatt gatgatgctg actggtttaa ggttgcttgc
     1381 aaaagacctc aagattttaa atggcaaata cattcagcta gaacccatta ccagccagca
     1441 ccgtgaggag ttgcgaaaag cagcaaatca tgaacaaatt tggcaataca tgccgcaaaa
     1501 ggcaacgggc aagttattta attcctggtt tgatgattgt cttgagaaaa tgtccataag
     1561 agaccaaatt acttatgcag tgcgttgtaa aaaagaaggg gatctgaaag gagctacttc
     1621 attttacgat attcaaccag aaaacaaacg gctggcttta ggctatagtt ggtacattcc
     1681 taaagtatgg ggaacaacgg caaacccgga atcaaaatta ctcatgttgc atcaggcgtt
     1741 tgaggcctgg gggattaatc gagtagaact gggtacagat tctcgtaata ttcattccta
     1801 tcgtgctatc aaaaagctgg gtgcgacgga ggaaggggtg ttgcgccaac atatgattct
     1861 tcataatcag gtgataacgg atactattat gtttagtatt cttttctccg agtggccagc
     1921 ggtaaaagag cgattaatac agagattgaa ctatgctgca accgataaag acctcttggc
     1981 tgtctcgata tagtaatccc ttgttctctg ggttaaacag ctcaactcat gaaataatag
     2041 acacacaata aatagaatga aaaaagattg gtatttttct ttaattgagt gaatatttgg
     2101 ttctacctgc gagaaaggag tgctcaatgt acaacaaaat gtttaagcct ctggatactg
     2161 atccgatttt atatttcaaa atgtacagta attatactga gggaagggtt gatgattgtt
     2221 gtgcatttat tttaatgccc agtgggttac aaagagaatg ggtctgtttg caatcgattc
     2281 aatttgcttt taataagcgt ggtgacgtcc ttgggattaa cattattttt tctggaaatg
     2341 agtcgaatat ccacaaaaag gttcgagaaa cgatggaagg gatgctcaag ctgaagcttc
     2401 aatatggaag aggggaagag cttttcgttt ttgatgaaga aaagaaaaca tttcatatgg
     2461 ggatagttcc tggtaaggat acacaggcgt atttggaggg cattatcgct tttattaagg
     2521 atagttatcg cttgcaaccg gactttgccc aagacatcaa gtcacaactt cttagtaagg
     2581 aatatctggc acaggaatat agtcgtttgc gatggaagcc accagagaaa gaaactgttt
     2641 gtgtgcttat gtaggttgta gaagcattgt ttccacttta aaaatagaaa aatttgaatt
     2701 ttatggggac tattgcattc cattagcttg gtttgcgtaa tactctcttg acaactaaaa
     2761 gattgaaagc tcaatattct ttatttacac caaggttcag gattttcatt gtataggaag
     2821 cgatggctca ttttattgtg tgctttgttg aataatggtt gcctctggct tgggtcagaa
     2881 tacaggaaac ctcatgtcaa caccccgcaa aaatggccgc ataaatattc aattcaaact
     2941 gagcaagctg cctatttacc tgatctgcat tggtggaaac agttcaatag caaggagctc
     3001 aatgcttcta tccaaaaggc attacaaaat aatcaccaga ttcacctcgc tatggcaaat
     3061 attgaatccg cacaggggca attgcagcaa gtccagttaa gttggttgcc taatctcaca
     3121 gggcttgcag gctacaccca gtttcccgtt ttaggtaatc cggggactac ggcaattgcc
     3181 tatccagcct acatcattaa tattttccag caatataagc aacaaaaaag cgcgcaagcc
     3241 atgcttgaag ccagtattta cgcgcaatat tctgccagac tggtcgtgat agcccagact
     3301 gcagccagct tttttacctt ggttgctcag aatgaagctt tgcatttata caataaactg
     3361 cttaaggatt atcgaaccta tctcaaactg actcaaagcc aatatcgctc aggattaatt
     3421 tcgcttgata atatcactca aatcaaaagt catatccaac gaattgaggc acagatcaag
     3481 gtggttcagc ataacattgt ggtgactaaa aacgccttgc attttttatt taatgaaaat
     3541 cctggagatg tcgagactaa aactctcttt gaaaatatcg atagtaatca attggtacca
     3601 gggaatttac cagccagcgt tctcagagtg agacctgata ttcatgaggc agaagcgttg
     3661 ttaaaagcag cgcatgccga tgtgggtgcg ataaccgcga atcttttacc agggattaat
     3721 ttgggcgctt acctgggtga gggctccaat gtggatggcg ccattaaatt agggcaagcc
     3781 tatttaaacg gcccgataat tgatttgcct ctatttgctc aggttgatgt aagtaaagct
     3841 cgatataagg caatctatat caaatacatt accactatcc gtaaggcctt gcgtgatgtt
     3901 gctaatgact tgtctgccta ttcaacttcc agccagcaat taaataataa tttatcggct
     3961 ttaaacgatg aaaaacagca atgccatttg gcagaagttc gttatcgtca cggaattgat
     4021 gactatttaa atttaataaa atgccaaatc ctgttagacg aatttaaatt ggtgataaac
     4081 cagaacaagc tggaaaaact gctatccata gtcactttat accaggattt aggaggtggt
     4141 taccgtgggt attgatcagc aaaagaccgt ccgtaccttg cgcttatccc tgacgtgtat
     4201 ttttcttttt ctaattacct ggtactacca agtcccagaa agcgcatgga ctttagtgac
     4261 catatggttt gtcatgtatg aatattccac cgttggtggc gtactgacaa aaagcttttt
     4321 gcgcttcgca ggcacggctt tgagtgccat ctatggaatg atagttgttt atttttgtgc
     4381 caataacccg ttgattaaca ttatggcttt agtacctggt ttgtttcttt acgcctattt
     4441 tttcatgggg ggagataaaa cgtatattgg caccattggg gctgtaacgc ttactattgt
     4501 attgctgaat tataacgaca tagatacggc tgtattgcgg gtattcaatg tcattatcgg
     4561 tgtcattggt tctatgttca tgatccggtt tttttatccc caatatgcaa gagataaagt
     4621 tttagagatt cagttgaact ttatcactca acttgcaaat cttttagaaa gctatttgaa
     4681 tccaacccag tctttggcag ctgtccagac aaagtattta gattatgaac gcaagatgct
     4741 tgatggtttt actttataca atcgttatat tggcgaagcc aaaatagaaa caaaaaaagc
     4801 tccgtttttc atcccccatt ccattgctgc catgcaacat tttaggcgtc tgtttcgttt
     4861 gttcagtgta tttgtttatt atttatctac tgaggaaatt cgatctgatc catgggtatg
     4921 tgatcaatta agtaaacttc tgtgtaattt acagtcactg caaagaaaat tgctgaaaca
     4981 agatgcgggg cccatagggg gagacgttgt ggctgcagag ccag
//
LOCUS       DAXLDT010000202         5000 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXLDT010000202 DAXLDT010000000
VERSION     DAXLDT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.203"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(272..1024)
                     /locus_tag="ACF2SP_002807"
     CDS             complement(272..1024)
                     /locus_tag="ACF2SP_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360574.1"
                     /translation="MFSKILKSRPNFFKAISEICNPGIGDKILKLQKPSLLIRIDGRS
                     PDSLHIQDGFQPRVSGERLKSVTASDVEDYQKFNVNPFGWGACQSLKEIMTFMQKNKS
                     QTDKGWLYKFFGKATPLLILKQEVGADSHGHDDEKEVIITEHIPLKNFIASIAPEDRE
                     MFMDGREIPNAMYSYNSSLPKSLSKQDLLTEQDILAWLLRHDAEEPFTDLCQTVFTDM
                     PQDILEYLFQENTGIVSAITSAIEKNMVLNLL"
     gene            1571..3283
                     /locus_tag="ACF2SP_002808"
     CDS             1571..3283
                     /locus_tag="ACF2SP_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360575.1"
                     /translation="MNVKPNIQTTLQNGKPLPYQKIGNQKVASFKDPNKPPYGPFANT
                     TNAGEFPLKQTVLIHGIEYSFTWPSSEHAFHAQKLIHLMNKLGINDPNQIHILNTLRK
                     IEKTQNQAGKQFDPRENWDPIARELATNMGITGTDQDKKDKIDELCDSNYYSKALVKK
                     GIVPGTKEPYTRRFMATVLRLKCDQNPELKELAMRCASEGILPIEASTKDVNWASGID
                     GSGRNMLGIEILKLGNQYLMEAGRGNEIKIADPDKAYEDLQRSHQKDLGHDAIIGKPW
                     SNPLNNSLNIWYDSTRKQTVVESHRGSNLQLIYKDGEPPKAIWRKDENSPWQDGDFKR
                     PAVQSLLQAYQDAGSPVPTVAKQQPHPAQLNDRWAKVHKQHHDNPVINLPLFKQELRE
                     GVKLELNKIEAELKKGINPLFSRNESHLERIVREYVNPAVLEASKILAAYSVKNAMHD
                     LNTSVTVSMVDNNRAHDDKHPLHAMKIQFKDNAEAKQFAEKLLNEYGIHSHTFGKGIM
                     KTPQNGAIYLTPKDLQMLSQLSGLAKDPLAGLQAFANMAKNYDKAKEVSIKEDLESHA
                     GLSP"
     gene            complement(3527..3817)
                     /locus_tag="ACF2SP_002809"
     CDS             complement(3527..3817)
                     /locus_tag="ACF2SP_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFD2360576.1"
                     /translation="MHPSYVYILASQRNGTLYIGSTSDIINRVWEHKNKVIPGFTAKY
                     NVHMLVYYEMYDLYIDAARREKRFKNWPRQWKINLIEKFNPEWRDLYEEICQ"
     gene            complement(3868..4617)
                     /locus_tag="ACF2SP_002810"
                     /pseudo
     CDS             complement(3868..4617)
                     /locus_tag="ACF2SP_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611762.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            4760..>5000
                     /locus_tag="ACF2SP_002811"
     CDS             4760..>5000
                     /locus_tag="ACF2SP_002811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360577.1"
                     /translation="MPTDPRYWYCFSVNRELVVFAKIFPFPSPQPSPAQGIIMLLKLL
                     AQEKEQIGVKLNNPCSIQDSILSKKLQGLCLYKSII"
BASE COUNT         1603 a         1005 c          920 g         1472 t
ORIGIN      
        1 agagattaag atcgtcgcaa aacactcgat tcgcgacttg ttccatgaaa aataaccatt
       61 taaaatatct gccagctcag tgatactcat tcacgactcc atgtcgtttc ttagcggaaa
      121 aaatatggat tatcaatgaa tcagtgagct acttcaacga attcaggcca ctggattgct
      181 tcgtcgctac gctcctcgca acgacggtcc caaaaatcgt accatacaga gctaaaaaac
      241 ttcagggacg ctgcttagct taaaataatt tttacagcaa attgagaacc atatttttct
      301 ctatggcact ggttattgca ctcacaattc cagtgttctc ttgaaacaga tactccaaaa
      361 tatcttgagg catatcggta aatactgtct gacaaagatc cgtgaatggt tcctctgcgt
      421 catgtcttag taaccaggca agaatatctt gctctgttaa taaatcttgt ttactcaatg
      481 attttggcaa actgctgtta taactataca tggcattagg tatttctctt ccatccatga
      541 acatctccct gtcttcagga gcaattgagg caataaaatt ttttagtgga atatgctctg
      601 tgataatgac ttccttttca tcatcatgtc catgactgtc agctcccacc tcttgtttca
      661 aaatgagaag tggcgtagct ttaccaaaaa acttatacaa ccagcctttg tctgtttgtg
      721 atttattttt ctgcatgaat gtcattattt cttttaaaga ttgacatgca ccccatccaa
      781 aaggatttac attaaatttt tgataatctt caacatcaga ggcagtaaca gatttcagcc
      841 tttctcctga tacacgcggc tgaaaaccat cttgtatatg tagagaatca ggggagcgcc
      901 catctatgcg tatcaataaa gatggctttt gtaatttaag tattttatca ccaatccctg
      961 ggttgcagat ttcggatatg gctttaaaga aatttggtct ggattttaga attttggaaa
     1021 acataataac ctcataataa agaacaatca atgcattttt ggttgatttt aattcatgtt
     1081 gattaattaa ttattatttg gttatgacat ttctggaggg ggttatacgc aaatcacaaa
     1141 aacttataag aaccctaaat ttctgaattg tgaacaatcc tgttccagag aagagagtga
     1201 aaaagatcaa atgcgagtgt ctcaaaaaat gagctcagat gcctaaatcc ttttcattaa
     1261 ttcggtaagt tcgcaatgcg ttacgaaatt agctcatgat tggccaggaa ttattacatc
     1321 acctgccaag gccgcatgat cagagcttaa agtgtaaccc ttcaaacgct atagctgcct
     1381 gatatcgatt aatgacataa gctattgcat tacctgtccg gatccatgaa agttttgagg
     1441 acgataatat gcatagctat ccatgattgt cataccccat gaagtattcg tatcgaaacc
     1501 taatactgca ttaatctcga tgcgttacat tgtaactact ggcaatgatt atactttgga
     1561 gagcgaaatc atgaacgtaa aaccaaacat acaaacaacc ttacagaatg ggaaaccact
     1621 accctatcag aaaattggga atcaaaaagt cgcctcattc aaagatccaa ataaaccacc
     1681 ttacggtcct tttgctaata caactaatgc tggtgaattc ccattaaagc aaacggtgtt
     1741 gatccatggc atagaatatt cattcacttg gccctcctcc gaacacgcat ttcatgcgca
     1801 aaaactaatc cacttaatga ataagcttgg tataaacgac ccaaatcaga ttcatattct
     1861 taacacttta aggaaaattg agaaaacaca aaatcaagcg ggaaagcagt ttgatccaag
     1921 agaaaattgg gatccaattg caagagaact agcgactaat atgggtatta cgggtacaga
     1981 ccaagataag aaagataaga ttgatgagtt atgtgattca aactattact ctaaagctct
     2041 tgtaaagaaa gggattgttc caggtaccaa agaaccttac acacgtcgat ttatggcgac
     2101 tgtattacgt ctcaaatgtg atcaaaatcc tgagttgaaa gaattagcta tgcgttgcgc
     2161 cagcgaagga attcttccta tagaagcaag tactaaagat gttaactggg catctggaat
     2221 cgatggttct ggcagaaata tgttgggaat tgaaatatta aaattaggca atcagtattt
     2281 aatggaagca gggcgtggaa atgagattaa gatagctgat ccagataaag cttatgaaga
     2341 tctccaacgt tctcatcaga aagatttggg gcatgatgca ataatcggta agccatggtc
     2401 gaatccttta aataactctt taaatatctg gtacgactcc acgagaaagc aaaccgtagt
     2461 ggaatcccat cgaggttcta atctgcaatt gatatataaa gatggagaac cgccaaaagc
     2521 aatatggaga aaagatgaaa actcaccgtg gcaagatggg gattttaaac gtcctgctgt
     2581 acaaagctta ttacaagcat accaggatgc tggctcgcct gtaccaactg tagcaaagca
     2641 acaacctcat cctgctcaac tgaatgatag atgggctaaa gttcataaac aacatcatga
     2701 caaccccgtc attaatctac ccttattcaa acaggagtta cgtgaaggag taaaattgga
     2761 gttaaataaa attgaagctg aactgaaaaa gggaatcaat cctttatttt cccgtaacga
     2821 gagtcatctc gaacgtatcg tcagggaata cgtaaaccca gcagttttgg aagcatcaaa
     2881 aatattagct gcttacagtg taaaaaatgc tatgcatgat cttaatacct ctgttactgt
     2941 cagcatggtt gacaataatc gggctcatga tgataaacat cctttgcacg cgatgaaaat
     3001 tcaatttaaa gacaatgcag aagccaaaca atttgctgaa aaacttttaa acgagtatgg
     3061 tattcatagt catacttttg gaaagggcat aatgaaaaca cctcaaaatg gagcaatata
     3121 cctgacacct aaggatttac aaatgctgtc gcaattatcc ggactggcaa aagatccatt
     3181 ggctggtctt caggcatttg ctaatatggc aaaaaattat gacaaggcaa aagaggtttc
     3241 gataaaagaa gatttagaaa gccatgctgg cctgagtcca tagttctctg caaaagggtg
     3301 attagtgtga accctataga actaatcacc tcaattttgt cattatgcaa tgcagttcaa
     3361 ctaataagaa tcaatcctca ctgagcaata tcaagtgagg atttttcttt tagagttttc
     3421 ggagcataat aacatctcaa caggatgacg tggtcactag ggtctgttga catttcacct
     3481 gccgcctacg tgccgcggct tgtccgcggc atccattaga tctaaatcat tggcaaatct
     3541 cttcatacag atcacgccat tctggattaa atttctcgat taaatttatt ttccactgtc
     3601 ttggccaatt tttgaaacgt ttttcacgac gagctgcgtc aatatataag tcatacattt
     3661 cataatagac aagcatatgt acgttatact ttgcagtaaa gccaggaatg actttatttt
     3721 tgtgctccca gactcgatta atgatgtctg acgtagaacc tatgtaaaga gtaccattac
     3781 gctgacttgc gagaatataa acataagatg gatgcatcat atttccttgg atgttggaca
     3841 aacatccaga taatactgca agaaatgcta catagttagc cataaaaata aatacgccat
     3901 agcaacaaca gaagcataat tttgctttaa tttatcaaat cttgtagcaa ttgcacggaa
     3961 atgctttatt ctagcgaata aattttcaac taaatgccta tgtttataaa gacaccagtc
     4021 catatctata ttgcaacttt agaattactc tttcttggaa taactggagt ggatgatttt
     4081 ttacgaatac tgtctctcgc ttcttcactg tcatagcctt tatcagcaat ggtgtgacct
     4141 gctgcagcca gtttttcaat aaattctgga gccactttac aatcgtgcac ttcacctcca
     4201 gttatttcaa agtcgatggg taacccatga gcatcaacag ccatatgaat ttttgttgta
     4261 ttgccccctc tagattttcc tatagcttga ttttcttcag aagctccgcc ggcactatgt
     4321 tggtgcgctt ttacaatgct gccgtcaata aactcccatt caagatctgg tttttgaaca
     4381 agttctttaa ataggcacat caatttacct tttgatgacc atcgattaaa tttttgatag
     4441 attggagttc caacatccaa attatgatgg caggtcgcgc caagggcagc caacccgtat
     4501 acgatataac attgcctcca ctaccatacg aagattaggc ttgtcataaa tcccatgctg
     4561 tagcatgctc tcctttagct tcgaccaaaa ctcatcattg agcattagtc ttagcattgc
     4621 aaactcgttt tattcttggg gtcagaaccg ttatattatg agtttgctct tattattcaa
     4681 tgaattaggt agtgcggttt ttgatttgaa catctactgg atgccgcgga caagccgcgg
     4741 cacgtaggcg gcaggtgaaa tgccaacaga ccctaggtat tggtactgtt tttccgtaaa
     4801 tcgcgagctc gttgtctttg caaagatttt cccattcccc tctccccaac cctctcccgc
     4861 gcaaggcatc atcatgctat taaaactcct ggcgcaggaa aaggagcaga tcggagtgaa
     4921 attgaataat ccgtgctcaa ttcaagattc aatactgtct aaaaaacttc agggactttg
     4981 cctatataaa tcaattattc
//
LOCUS       DAXLDT010000203         4947 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXLDT010000203 DAXLDT010000000
VERSION     DAXLDT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.204"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..306)
                     /gene="rlmJ"
                     /locus_tag="ACF2SP_002812"
     CDS             complement(<1..306)
                     /gene="rlmJ"
                     /locus_tag="ACF2SP_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFD2360578.1"
                     /translation="MLSYQHGYHAGNFADVIKHITLTRLLAYLTHKDKPLFYLETHSG
                     RGIYDLKDKQSLKTEEYKEGINPVWLDRENLPSLFLEYISIIKQINLNSTLRYYPGSP
                     "
     gene            592..801
                     /locus_tag="ACF2SP_002813"
     CDS             592..801
                     /locus_tag="ACF2SP_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360579.1"
                     /translation="MGLHEEFEDQSFSDYNYDEEIDDHIDDLNQKKKIRRMLEDRLER
                     KRLKEEFKDDFDELSGEFDWDELDK"
     gene            complement(804..1658)
                     /gene="folD"
                     /locus_tag="ACF2SP_002814"
     CDS             complement(804..1658)
                     /gene="folD"
                     /locus_tag="ACF2SP_002814"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFD2360580.1"
                     /translation="MPASLIDGREISALRRNELKQRVQHHVGLGQRPPGLAVVLIGND
                     PASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLIQLINELNQSDKIDGILIQLPLP
                     KHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
                     HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIAT
                     DWLREHQVVIDVGMHRLPDGSIRGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTM
                     MSAARLRE"
     gene            complement(1745..1945)
                     /locus_tag="ACF2SP_002815"
     CDS             complement(1745..1945)
                     /locus_tag="ACF2SP_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360581.1"
                     /translation="MNRENEVIEIFLMDISKKEKCKLLQDFLLDCKNEMEAQDQNMHP
                     EVHHNLSQAYQLAQNYLRKLQE"
     gene            2214..4907
                     /locus_tag="ACF2SP_002816"
     CDS             2214..4907
                     /locus_tag="ACF2SP_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HFD2360582.1"
                     /translation="MKKTVLHAAIFSLTMPISLYALGLGEMKVQSSLDQPFSAEIELI
                     DVGADPLASIKVSLADPEQFEQIGLERTAVLSLLRFQVERNPKGAPVVKVSSTERMTE
                     PYMEIVVDLTWPKGQLYKAYTVLLDPPGYQLGITTAQSRPVYYKKKSASYSNEPGVIE
                     KTVITTVEHGPVSKNDSKKKTTYGPTVTNENVWQIAQRYKTSKLILPQVVLAIVGANP
                     EAFKDGNLNGLKVGVRLVIPSTEEIAKVPSDLAIEEVMAHDKAWNEHTSINHVLAPPY
                     INSPIADMTPKQTKASSIPVIPKFTIGENSNIPTLQQLIPDSTSVSIDTSTQQQPVQN
                     SNIVQTTGQNEKIKAEISITTAAVESVREANALLMEQLRILQEQNKKLQEKLDKREKE
                     IELMRTQVQTMLKERKAIASQASSLPNNEQGMNLWPYIILLIFAAGGAGFAYWYLRQR
                     QHREDISPYKTDSDSSPKPFMPLVEPAKYEPDTQADDKETSVQEKVSDSEKETGVGVS
                     KADWTEYQPKEMDKIEKADAVESETVEDVTYLGGTEQLTAKQDTELEPEVQTKPEVQT
                     KPEVQTKPEVQTKPEVQTKPEVQTKPEVQTKPEVQTKPEVQTKPEVQTKPEVQTKPEV
                     QTKPEVQAKPEVQAKPEVQAEPEVQAEPEVQAGENQIMFEPVMEPEQLDVNRDDEVDN
                     SQLSEDETQQTEGHQSSEMIEFESGLHELIKDKPKTKITESTDEGDDNGIDFIPLTIN
                     DADKTLKHEQEIEKSDKKEDKKSEEFSTKENKFDLDNSDFDMENLESEPEISQEEKED
                     HISEITEFDQKSTQEEKTEKGISISQSVDKATFTKSSGENNETSNPLKSKKALDTLLD
                     LAKTYISMGDIETARSSLEEVIEHGSANQKEQARQLLDQLK"
BASE COUNT         1660 a          890 c          984 g         1413 t
ORIGIN      
        1 gggggagcca ggataataac gcaatgttga attaagattt atttgcttaa ttatactaat
       61 atattcaaga aaaagagatg ggagattctc tctatcgagc catactggat taattccttc
      121 cttatattct tctgttttta aagactgctt gtcttttaaa tcatatatcc ctcgacctga
      181 atgggtttca agatagaata agggtttatc tttgtgggtt agataagcca atagtcgagt
      241 taaggtgatg tgcttgatga catcggcaaa atttccggca tgatagccat gttgataact
      301 gagcatgaga gggcttcaaa agatattttc caaattatat caaaataatg gtttatgctg
      361 tagccgcata ttagacttct tgcataaacg gtctctttta ttttactccc gcatcgtaaa
      421 catttcttca acacttatat actgcatgta aactccgtct ctcaaaacgt atgtgccatg
      481 ccctgaagca aaataatcag gttatacaag cggcctattt tgcttccagt tactttattt
      541 cataatttat gcactatact ttttttgagg ggagtaaatt gggagactaa gatgggttta
      601 catgaagagt ttgaggatca atcattttca gattacaact atgacgaaga aattgatgat
      661 catatagatg atttaaatca aaaaaagaaa atcaggcgta tgcttgaaga taggcttgaa
      721 cgtaagcgtc taaaagaaga gttcaaagat gattttgatg agctaagtgg tgaatttgat
      781 tgggatgaac tggataaata acttcattct cttaatcttg ctgctgacat catagtattt
      841 tcaagcaaag tgacaatagt cattgggcca actccccctg gaacaggtgt gatccaggct
      901 actttttcga ctgccttctt aaaatcaata tcacctctga tactgccatc gggcaaacga
      961 tgcatcccta catcaatcac tacttgatgc tctctgagcc agtcagtggc aatgacgtcc
     1021 atctttccag tggctactat tagaaaatca gcaatctcaa caaacttctg taattgttgg
     1081 gtgaacttgt ggcatattgt tacggtagcg ccagccaata gtaactccag gctcattggc
     1141 cttccaacaa tattggatgc cccaatgacc acggcatgtt ttctttttac attgagctca
     1201 tagtgatgta ataaattcat aatccccaag ggagtgcatg gtcttaaaaa tgggtttcgt
     1261 tgagcaagtc ggcctaaatt atagggatga aatccatcca cgtctttctc gggctttatg
     1321 tgttctatta tagttctttc gttaatatgt ttgggcaaag gtaattggat taaaatacca
     1381 tcaattttat ccgattgatt taactcattg atcagttgga ttaatttttc ttgagtggtt
     1441 tcagcaggta aatcgtaaga atgagaggtg atccctactt cctcacaagc ttttcgttta
     1501 ttactgacat aaatcactga ggcagggtca ttgccaatta aaacaacagc aagccctggc
     1561 ggtctttgcc ctagccccac atggtgttgt actctttgtt ttagctcatt tctgcgaagg
     1621 gcagagattt ctcttccatc gattagtgat gcaggcattt tcttctcgca atttttatgg
     1681 agtacattat gaattacggg ttattctaat gcaatatgat tcatatgcaa caaggaaaat
     1741 acacttattc ttgcagtttt cttaaataat tttgcgccag ctgataagct tgcgataaat
     1801 tgtgatgcac ttcaggatgc atgttctgat cctgagcttc catttcattt ttgcaatcca
     1861 gaagaaaatc ttgtaacaat ttgcattttt cctttttgct gatatccatt agaaatattt
     1921 cgatcacttc attttctcta ttcatcgcta ttccaaattt aattttacta atccttaata
     1981 taattatagt tgaaggtcta ttggagttat gaattttcct ggttggattt taaaaggcca
     2041 caattgattt ggcttgttaa attagatgaa atatgtggtg taattttata catcatctta
     2101 tgatttttag ggaataattt cgtgttttct tgaatagact aacggataat gatacttttt
     2161 acttaggcaa ttaaatcatc aacatcttgt atcacaggtg catggagctc tgattgaaaa
     2221 aaactgttct gcatgcggct atattctcat tgactatgcc aatatctctt tatgcgcttg
     2281 gcttagggga aatgaaggta cagtcctcat tggatcagcc tttttcagcc gaaatcgaat
     2341 taatagatgt aggagcagat cctcttgcca gtatcaaggt gagtttggct gatccggagc
     2401 aatttgaaca aattggactt gagcgaactg cagtcttgtc tttgttacgg tttcaggttg
     2461 agagaaaccc aaaaggtgcc cctgttgtaa aagtatcgtc tacagaaaga atgacagaac
     2521 catatatgga aattgtcgtc gatctaacct ggccaaaagg tcaattatac aaggcctata
     2581 cagtattatt agatccgcca ggatatcaat tgggaattac caccgctcaa agcaggccag
     2641 tttattacaa gaagaaatcc gcaagttaca gtaatgagcc tggtgttatt gaaaagactg
     2701 tgattactac agtggagcat ggccctgtat caaaaaatga cagcaaaaag aaaacgactt
     2761 atggtccaac tgtaaccaat gaaaacgttt ggcaaattgc tcaaagatat aaaacttcaa
     2821 aattgatttt gcctcaggtt gtattagcaa tagttggtgc gaatcccgaa gcatttaagg
     2881 atggaaattt aaacggttta aaagttggcg ttcggctagt tattccatca actgaagaaa
     2941 ttgcaaaagt gccttctgat ttggctattg aagaggtgat ggctcacgac aaagcctgga
     3001 atgaacatac atctattaat catgtattag ctccgccata catcaacagt ccaatagcgg
     3061 acatgactcc aaaacagact aaagcctctt caatcccggt aattccaaag tttactattg
     3121 gagaaaactc taacattcca acacttcaac aattaatccc cgatagcact tcagtttcga
     3181 tagatacatc tacccagcag caacctgtgc aaaacagcaa tatagtacag acaacggggc
     3241 aaaatgaaaa aattaaggca gaaatttcaa ttacaacagc agcagttgaa tcagttcggg
     3301 aagcaaatgc attattgatg gaacaattac gaattctgca agagcaaaat aaaaaattgc
     3361 aggaaaagct ggataagcgt gagaaagaaa tagagttaat gcgtacgcag gttcaaacca
     3421 tgttgaagga gcgtaaagcc atcgcgtctc aagccagttc acttcccaat aatgaacaag
     3481 ggatgaacct ttggccgtat atcattttat tgatatttgc tgcaggagga gcaggatttg
     3541 cttattggta cctcagacaa cgccaacaca gagaggatat ttctccttac aaaacagatt
     3601 ctgattcaag tccgaagcca tttatgcctt tagttgagcc agcgaaatac gaaccagata
     3661 cccaggctga tgataaggaa acttccgtcc aggaaaaagt atctgacagt gaaaaagaaa
     3721 ctggagttgg tgtttctaaa gcggattgga ctgagtatca acctaaagaa atggataaaa
     3781 tcgaaaaagc tgatgcggta gaatctgaaa cagttgagga tgtgacatat cttggaggta
     3841 cagaacagtt aacagcaaaa caagatacag aattggaacc agaggttcag actaaaccag
     3901 aggttcagac taaaccagag gttcagacta aaccagaagt tcagactaaa ccagaagttc
     3961 agactaaacc agaagttcag actaaaccag aggttcagac taaaccagag gttcagacta
     4021 aaccagaggt tcagactaaa ccagaagttc agactaaacc agaagttcag actaaaccag
     4081 aagttcagac taaaccagaa gttcaggcta aaccagaagt tcaggctaaa ccagaagttc
     4141 aggctgagcc agaagttcag gctgagccag aagttcaggc tggagaaaat caaattatgt
     4201 ttgagcccgt tatggagcct gagcaattgg atgttaacag ggatgatgag gttgataatt
     4261 ctcagttaag tgaagatgaa actcagcaaa ctgaaggcca tcaaagttct gagatgatag
     4321 agtttgagtc aggtttacat gaattaatca aagataagcc caaaactaaa ataacagaat
     4381 caacagatga aggagatgat aatggaatag atttcattcc tttaactata aatgatgctg
     4441 acaaaacatt aaagcatgaa caagaaattg agaagtcaga caaaaaggaa gataaaaaat
     4501 cagaagaatt ttctacgaag gaaaataagt ttgatttaga taattcagac tttgatatgg
     4561 aaaacctcga atccgaacct gaaataagcc aggaggaaaa agaagaccac atttcagaaa
     4621 taactgaatt tgaccagaaa tctactcagg aagaaaaaac agaaaaaggt atttctatat
     4681 ctcaatctgt tgataaggcg acattcacca agtcatccgg tgaaaacaat gaaacctcca
     4741 atccattgaa gagtaagaag gcattagata ctttgttaga tttggcaaaa acctatatta
     4801 gtatgggcga tatagaaaca gcaagaagtt ctcttgagga agtgatcgag catggaagtg
     4861 ccaatcaaaa agagcaagcc aggcagttac tggatcaatt aaagtaacaa ataacacaaa
     4921 aattacgccc ccaatctctt aatattc
//
LOCUS       DAXLDT010000204         4855 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXLDT010000204 DAXLDT010000000
VERSION     DAXLDT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.205"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            119..970
                     /locus_tag="ACF2SP_002817"
     CDS             119..970
                     /locus_tag="ACF2SP_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360583.1"
                     /translation="MKKFAITLMSLIFINLYHPTAFSEKPNQRMMNNQATHSLKSKNT
                     LQLSIQEIMDKKDKKLVFIKLIDTKNNKPITLNDLIEAHTQKIHLLIIDDSLLDYSHV
                     HPVETTIPGIYQFEWQPTLKSASYRAWADLIPTNTKTQEYVVADLPFPKTKKKLENPI
                     HRQPLFESSVDGYHFKLSFDKTPLHVGQAVMGKIDITDAKENPVHTLEPIMGAYAHIV
                     GFNEDFKTVTHVHPMGKEPTNNTERGGPELLFHIKPNKVGFIKMFAQVQIDGKTLFVP
                     FGVTVNS"
     gene            complement(1276..1893)
                     /locus_tag="ACF2SP_002818"
     CDS             complement(1276..1893)
                     /locus_tag="ACF2SP_002818"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFD2360584.1"
                     /translation="MSVKITLYRWEGQYGAFKIKVPCGECSLTKDIILDAIATDLKDI
                     PVELDIHAWLNEWWKPLLKGAWHAPIVLVEGKVISQGHALNRGVLTQAVIEAHAKQTT
                     LTGNHIFGKPHCIHCQHAKQYFEEAKLSYEFHDVVKNPRDLYEMLARVKPIVGAKTPI
                     TVPQIWLDSQYVGGAEELSAILGREVEPNPERGICSLTAKGHEKN"
     gene            complement(2067..2264)
                     /locus_tag="ACF2SP_002819"
     CDS             complement(2067..2264)
                     /locus_tag="ACF2SP_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HFD2360585.1"
                     /translation="MEIRTVAFESDLIITLEGNQKVVITPFKTLEPGNFKLGIEAPKN
                     ISINRQEIHLRKQEQLKKYEG"
     gene            complement(2364..2573)
                     /locus_tag="ACF2SP_002820"
     CDS             complement(2364..2573)
                     /locus_tag="ACF2SP_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360586.1"
                     /translation="MTLDNLIGISLEQMDVYQSDEVQDSFDGVKPSINGFYAIKKEPP
                     FLSVPTHGVAMVAVVSGAIFLEDCV"
     gene            complement(2560..2802)
                     /locus_tag="ACF2SP_002821"
     CDS             complement(2560..2802)
                     /locus_tag="ACF2SP_002821"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFD2360587.1"
                     /translation="MAKGTENLGSDIDVLIIGEVDFTEAVEALYAAQASLGREINPKI
                     YSREQWKASLQKQDLFIQEVLNNPKLFIMGAEHDLG"
     gene            complement(2786..3136)
                     /locus_tag="ACF2SP_002822"
     CDS             complement(2786..3136)
                     /locus_tag="ACF2SP_002822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFD2360588.1"
                     /translation="MFDTSILFSEYRRRVLALLLLHPDERYHVREIARLTNTTAGTLH
                     RELSKLAKSKVLLREQSGNQVYYQANRNFPIYMELTSILKKTSGLVDVLFDFLTPLAE
                     KIEVAFFLFGGQRN"
     gene            complement(3422..4303)
                     /locus_tag="ACF2SP_002823"
     CDS             complement(3422..4303)
                     /locus_tag="ACF2SP_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360589.1"
                     /translation="MKKDKEKHQPVLESITADEPKTSSIALALQQFRKEAEKKEAAEE
                     GAKVRCPTCFKEIAPKRLCSGHGAGGSGGDSATSDKKTSEEKASPGEDKFLTKSGKVV
                     GSEDELVGVFGSEELDLESGFDPEIIEKLIADGKLLVDSDRESMTLKLELQCDPNELT
                     PKQRHELKKFMEAIINEFSEFKEENHLSDDCIQILQDEEGNIHSLRITMPTLALYDAF
                     IQQLANNLVPVPSPKAQERDEVTKEQSFAPNPLSREPRPSNKDQSSTQEANEIHTKSE
                     DGEQEIFNPSPFNMKPW"
     gene            complement(4416..>4855)
                     /locus_tag="ACF2SP_002824"
     CDS             complement(4416..>4855)
                     /locus_tag="ACF2SP_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360590.1"
                     /translation="KNKVTWKEGHGKYPYKITPNSNLINILSAQGVALNTVINKGQQI
                     FIAKKVTMSSGAKISDVTDLIHPENKLLLEKAHKILNIPLTGLDFICQDISLPWHKQQ
                     FGIIENNSFPYIELHLNPSDGKGINVAGKIWDYVLDVLSQKNE"
BASE COUNT         1495 a         1015 c          814 g         1531 t
ORIGIN      
        1 tgtcttaaaa ggacaatcat gatatcattc tatatgctga tcaaaataaa tagcttacag
       61 aataggacta taagaaatta ttttataacc aattggtttt cttggagaca aaaatattgt
      121 gaagaaattt gccatcaccc taatgagctt gatattcatt aacttatatc atcccactgc
      181 tttctcggaa aagcctaatc agcgcatgat gaacaatcaa gcaacacatt ctttgaaatc
      241 aaaaaatacc ttgcaattat ctattcaaga gataatggat aaaaaggaca agaaactggt
      301 gttcatcaaa ttaattgaca caaaaaacaa taaaccaatc accttaaacg atcttattga
      361 agcacataca caaaagattc atttgttgat tatcgatgat agcctccttg actacagtca
      421 tgtccatcca gtcgaaacaa cgataccagg catctatcaa tttgaatggc agcctacgct
      481 taagagtgcc tcctatcgag catgggctga tcttataccg actaatacaa aaacccaaga
      541 atatgtagtt gccgatttac ctttcccaaa gacaaaaaag aaattggaaa atcctattca
      601 tcgacaacca ttatttgaaa gtagtgttga tggctaccat tttaaattgt cttttgataa
      661 aacaccattg cacgtcggtc aagcagtcat gggcaaaatt gacatcaccg atgcaaagga
      721 aaacccagtt cataccttag agcccattat gggtgcttat gcccacatcg ttggttttaa
      781 tgaggacttt aaaacagtaa ctcatgttca tccaatgggc aaggagccta caaataatac
      841 ggaacgagga ggacctgaat tgctatttca tataaaacct aacaaagtag gatttattaa
      901 aatgttcgcc caagtgcaaa tcgatgggaa aacactgttt gttccgtttg gagttacagt
      961 aaattcataa aaatcttgct ggaaaatccc cagaataatg atatccacca attgaagagt
     1021 taaggcgatt aaaggtggca ttgcagcact tactgcatcc gtgtaaacag catcgaccga
     1081 ataacctctg cccttctttt caccacattg gagcctaagg agaatcatca tgactaattc
     1141 acatggaact atgattatct taggcggtgc aataataatc agcacaagaa aataattact
     1201 ttttctccag tctttggtat ctttaatcac attgtctttg caagcagaag acgttgaact
     1261 gatttaatca gtggtttaat ttttctcatg gccttttgcg gtaagagagc aaatccctcg
     1321 ctcaggatta ggctcaacct ctcgcccaag aatggcactt aattcctcag ctcctccaac
     1381 gtattgacta tcaagccata tttgaggaac agttatcggt gtttttgcac caacaatggg
     1441 cttaacccgt gccagcatct catacaaatc ccttggattt tttaccacat cgtgaaattc
     1501 ataagaaagt ttggcttctt cgaaatactg tttggcatgc tggcagtgga tgcaatgggg
     1561 tttaccaaaa atatgattcc ccgttaatgt ggtttgtttt gcatgggcct caatcaccgc
     1621 ttgagttaac accccacgat tcagagcatg gccttggcta atcactttac cctcaaccag
     1681 tacaataggg gcatgccacg cccctttcaa taacggcttc caccactcat taagccaggc
     1741 atgaatgtcc aattcgacag gaatgtcttt taaatccgtt gcaatcgcat ccaagataat
     1801 atccttggtg agcgaacatt caccacaagg gactttgatt ttgaaagccc catattgtcc
     1861 ttcccaacga tacagtgtga ttttgactga catattagaa tggccttttc ttgttcccaa
     1921 ttaatgctca aaaataccca ttaacacgat caccatcata tcaatataga ggaacctcct
     1981 tttaattcaa tctactttct acctattgaa taattttttt cacctctttg cgatgcagca
     2041 gattacgaaa atagcaccag tcttccctaa ccctcatatt tctttaattg ttcttgtttt
     2101 cttaaatgga tttcctgtct gttaatggat atatttttag gagcctcaat acccaactta
     2161 aagttgccag gctcaagagt cttaaaggga gtaataacta ccttctgatt gccttccagg
     2221 gtaataatta aatcggattc aaaggctacg gttcttattt ccatgttagt ttactcgttt
     2281 tagttcaaaa taaatgctgc aaaaaagcat taattgatgg gttttcccta tgcgggcatt
     2341 ttaatcttaa agccaatgcc tgttcaaaca cagtcctcaa gaaaaatagc tccacttact
     2401 acagcgacca tcgcaactcc atgggtaggg acagacaaaa atggaggttc tttttttatt
     2461 gcataaaacc cattaatgct tggcttaaca ccatcaaatg aatcctgcac ctcatcggat
     2521 tgatacacat ccatttgttc caggctgatg ccaattaaat tatccaaggt catgttcagc
     2581 tcccataata aatagcttgg gattatttaa aacctcctga atgaaaagat cttgtttttg
     2641 taaagatgcc ttccattgtt ccctggaata tattttggga ttaatttctc gccctaaact
     2701 agcttgagca gcataaagag cctcaactgc ttctgtaaag tcaacctcac caataataag
     2761 cacatcaata tcacttccaa ggttttcagt tcctttggcc accgaacaaa aaaaaagcca
     2821 cttcaatttt ttcagctaat ggagttaaaa aatcaaacaa cacatccacc aaaccagacg
     2881 ttttctttaa aatgcttgta agctccatat aaataggaaa atttcgattt gcttggtaat
     2941 aaacttgatt accactctgc tcgcgcagaa gtactttgga tttagcaagt ttggatagtt
     3001 ctcgatgaag tgtacccgct gtggtattcg ttaaccgcgc aatctctcgg acatgataac
     3061 gctcatcagg atgaagcaat aaaagtgcca gcacacgccg ccgatattca gaaaacaata
     3121 ttgaagtatc aaacatgatt tttattttga agccaactaa gcttcaatca tagcctaaaa
     3181 agctacacca ttcaatcaga taaataggaa aaccagaaaa ataaatatca gttataaatc
     3241 gacgccccac tgaagagcat caacactaca accaatgacc tcaacgctct tgtgccaaga
     3301 cttaagggct ccgtcttgga gaaagcataa atggtatttc ccaattacgt aatcgtatta
     3361 ttggatacga aaggggatct tgaatagttc ttttcaataa tcaataaacc cctgctcttt
     3421 attaccacgg cttcatatta aaaggagatg ggttaaaaat ttcttgttcc ccatcttcag
     3481 atttagtatg tatctcatta gcttcttgtg tggaggattg atctttatta gacggcctgg
     3541 gttccctcga aagtggattt ggagcaaagc tttgctcttt tgttacctca tctctttctt
     3601 gtgctttcgg actgggtacg ggtactaaat tatttgctag ctgttggata aatgcatcgt
     3661 ataaagctaa tgtgggcatg gtaatgcgaa gagaatgtat gtttccctcc tcatcctgaa
     3721 ggatttgtat gcaatcatca gaaagatgat tttcttcttt aaattcactg aattcgttta
     3781 taatcgcttc catgaatttt tttaattcat gtcgttgctt tggagttaat tcattaggat
     3841 cacattgtaa ttcaagctta agagtcattg attcacgatc actatcaacc aacaatttcc
     3901 catcagcaat taatttttca attatttctg ggtcaaaacc agactctaaa tcaagttctt
     3961 ctgaaccaaa cactcctacc aattcatctt cagacccaac tactttgcct gatttagtta
     4021 ggaatttatc ttcaccagga ctcgcttttt cctcagatgt cttcttatca gaggtggcac
     4081 tatcacctcc gctaccacct gcgccatgtc cggaacaaag gcgcttaggt gcgatttctt
     4141 taaaacaagt cggacagcgt acttttgcac cttcctcagc agcctctttt ttttctgctt
     4201 cctttctaaa ttgttgtaaa gctaatgcta tagaagaggt tttgggttca tctgccgtta
     4261 tggattcaag gacaggctga tgcttttctt tgtctttttt catgagaata ctccagtgca
     4321 agttcataat ggtttcatcc gaataaagat ttacttatct gttattatac cgagttaaaa
     4381 aaacccaatt gtatataaat tgtacattta ttgggttact cgtttttttg acttaaaaca
     4441 tcgagtacat agtcccaaat tttacctgct acattaattc ctttcccatc agacggattg
     4501 agatgtaatt caatgtaagg gaagctatta ttttcaataa ttccaaattg ttgcttatgc
     4561 catggaagag aaatgtcctg acaaataaaa tcaagacccg taagcggtat attgaggatt
     4621 ttatgtgctt tttccaataa tagtttgttc tcaggatgta tcagatcggt aacatcactg
     4681 atttttgcac cactactcat agtaactttt tttgcaataa agatttgttg tcccttgttt
     4741 attaccgtat taagagcaac tccctgggct gacaaaatgt ttatcaagtt gctatttgga
     4801 gtaattttat atggatattt accatgacct tctttccacg taactttatt ttttt
//
LOCUS       DAXLDT010000205         4818 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXLDT010000205 DAXLDT010000000
VERSION     DAXLDT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.206"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            1..759
                     /locus_tag="ACF2SP_002825"
     CDS             1..759
                     /locus_tag="ACF2SP_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360591.1"
                     /translation="MFFKREKYTPISFIAILEKYDNNPVHQNITSSYDEELHTPAVLT
                     KYLSRDIKFLVAILEANKGHLAKDGELYKNITDALRFAEYDPERAKNNLNGVHSKRKK
                     IKNKNDINIESEWNATNDALKKCQTHFDRMESFKNWLDETEEEYWDKDTCKLLKSNIK
                     TLQNLADEITHLTKKANLIYQKYKDFWGENQENIEEKLDSKGQKIDKKEIEKFEKITS
                     SLVKLYHSLPAQHQFLDNFLQTVCLTMPTTKRLT"
     gene            complement(859..2193)
                     /gene="lem17"
                     /locus_tag="ACF2SP_002826"
     CDS             complement(859..2193)
                     /gene="lem17"
                     /locus_tag="ACF2SP_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem17"
                     /protein_id="HFD2360592.1"
                     /translation="MEDESKVKKGLKEEKEQLNQKWLEQLLNNPRWGPSVVKLAMARK
                     KENRNKRGEAFNCFVLSRPQNAFEEDEYRKFLDKLKNNINDFSGTTRFQIALFTADNH
                     HWTAFDFFIVDGKLHVFCLDAANDSAGDAALDEIMSRFPDCDAYRLEPDRDPKAKDSK
                     HFRLIQTDRENCSRMTIEHILLLSKKKFLFNENFANYKVVNGVKLINPDALFRQSPDL
                     YRMTQIDSILDSLMIASPKEAQRLVSKKGLNSEQYRDLLGHTSGKQSKSSKNTTIDYK
                     RQRVEKNLLAFLAESEQKHLEDIIDNSEKQWSEFLEMSQSERTQFLLGNIREKPFKEI
                     VGNILGKGIKDRLDHKKYIENDVIKKSSFTLFRGIDKHFEIKKIKAEINELEKALKIV
                     NQLSNDTSADEVLLHLQKQKLPLGIKAELEGALDIYTLLDKEKSALQSHKIN"
     gene            2424..3620
                     /gene="ralF"
                     /locus_tag="ACF2SP_002827"
     CDS             2424..3620
                     /gene="ralF"
                     /locus_tag="ACF2SP_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214205.1"
                     /note="RalF, a virulence factor secreted by type IV
                     secretion systems (T4SS), is a guanine nucleotide exchange
                     factor that acts on host cell ADP-ribosylation factors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS guanine nucleotide exchange effector RalF"
                     /protein_id="HFD2360593.1"
                     /translation="MHPEIEKAQREITEAFNAKPKNGIEKIKEICEEYKISPNEEIAA
                     FFHQQRKNLDLEAVGDYLSGPEAENQQVLKAFTSQMDFNGQSFVEGLRTFLKTFKLPG
                     EAQKIDRLVQSFSGAYSQQNPDVVSNADAAYLLAFQTIMLNTDLHNPSIPEKNKMTMD
                     GLKRNLRGGNNGGDFDAKFLEELYSEIKAKPFELNFVKTSPGYELNSTTLNKDSTFKK
                     LDSFLHSTDVNINTVFPGIGDNVKTTVDQPKSWLSFFTGYKGTITLTDKQTSAQATIQ
                     VYTPNILSKWLFGEQPKVIIQPGQTKESIDLAAKVAAGFSSPVKNFKATYDYEVGDLI
                     KAYDNQKKLISTNKNTLFKGSMFRKPNAEQQETSKSATRTLNTDYDINEDTVTRKDES
                     RSSYKK"
     gene            4116..4796
                     /locus_tag="ACF2SP_002828"
     CDS             4116..4796
                     /locus_tag="ACF2SP_002828"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFD2360594.1"
                     /translation="MTDIAVIINNRAKNAHRMEDYLSELRAHGIDYQLYKADPQQLEK
                     NIQHCCSKYSLLLIGGGDGTVRSAAQWCSNTSVILGVLPLGTMNHFSKELNLPSTTEE
                     LIAAIVEKTTTTIDVAEVNGHIFINNSSIGFYSRLAKNRDYYTNFYNKWLTYIPSFIR
                     ALSYHPSYALLIKNKELDISIRTSFFMVSNNCYTYEFPLKFTREVFKMSSWAFIITNA
                     ANCVYSNF"
BASE COUNT         1706 a          855 c          794 g         1463 t
ORIGIN      
        1 atgtttttta aacgcgaaaa atatacacct atatctttta tagcaatttt agaaaaatac
       61 gataataacc ctgtccatca aaatatcact tcatcgtatg atgaagaact tcatacacca
      121 gctgttttaa caaagtacct aagtcgggat atcaaatttc ttgtagcaat tttagaagcg
      181 aataaaggcc acttggcaaa agacggtgaa ttgtataaaa acatcactga cgctctgcga
      241 tttgctgaat atgacccgga aagagctaaa aataatttga atggcgtaca ttcaaaacga
      301 aaaaaaatta aaaacaagaa cgatataaat attgaatcag aatggaatgc gacaaatgat
      361 gcgttaaaga aatgtcagac ccattttgac agaatggaat cattcaaaaa ctggcttgac
      421 gaaacagagg aagagtattg ggataaagac acatgtaaac ttttaaagtc aaatataaaa
      481 actctccaaa atctggctga tgagattact catttaacaa aaaaggcaaa cttgatatat
      541 caaaaatata aagatttttg gggagaaaac caagaaaaca tagaagaaaa attagactcc
      601 aaaggtcaga aaattgataa aaaagaaatc gagaaatttg aaaaaataac atcaagccta
      661 gttaaactat atcatagcct acctgcacag catcaatttt tggataactt tttacagacc
      721 gtctgtttaa ctatgccaac aaccaaaagg ttgacttaat ttccaattta attgggcatg
      781 ttggagatta accaaaaacc atttaatgca aataaagagc atacttggtt ataaccagac
      841 ataaaatcca aaaataattc aatttatttt atgagactgt agagcagatt tttctttatc
      901 taatagagta tatatatcaa gcgccccctc caattctgct tttatcccca ggggtaattt
      961 ttgtttttgt aaatgtaaca atacttcatc agcacttgtg tcattagata attgatttac
     1021 tattttcaag gctttttcca gttcattaat ttctgcttta attttcttga tttcaaaatg
     1081 cttatcaatt cctctaaaaa gagtaaaaga actctttttg atcacatcgt tttcaatgta
     1141 ttttttatga tccagcctgt cttttatgcc tttgcctaga atattcccga caatttcttt
     1201 aaaaggcttc tctctgatgt tacccaataa aaattgggtg cgttctgact gagacatttc
     1261 taaaaactca gaccattgtt tttcagaatt atcaataata tcttccagat gcttctgctc
     1321 actctcggcc aaaaaagcta aaaggttttt ctccaccctc tgtctcttgt aatcgatggt
     1381 tgtatttttt gatgatttgg attgtttacc tgatgtatgt ccaagcaaat ccctgtactg
     1441 ctctgaattc aaacctttct tactgactag tcgctgcgcc tcttttggtg aagcaatcat
     1501 aaggctatcc aggatagaat cgatttgagt catacgatac agatctggag attgcctgaa
     1561 tagcgcgtca ggattaatca gcttcacccc attaaccact ttgtagttgg cgaaattttc
     1621 attaaataaa aatttctttt ttgacaacag caaaatatgc tcaatagtca ttcgtgaaca
     1681 attttctcgg tctgtttgaa tcaaacgaaa atgttttgaa tccttcgcct ttggatctct
     1741 atcaggctct aaccggtaag catcgcaatc aggaaatcgg ctcatgattt catcaagcgc
     1801 agcatctcct gcactatcat tagccgcatc taaacaaaaa acatgaagct ttccatcaac
     1861 gataaaaaaa tcaaatgctg tccagtggtg gttatctgca gtaaaaagag cgatttgaaa
     1921 tcgggtcgtt ccactaaaat cgttaatgtt attttttaac ttatccagga attttcggta
     1981 ttcatcttcc tcaaacgcat tttgtggtct actcagcaca aaacagttaa atgcctcacc
     2041 acgtttgttc cgattttctt tttttcttgc catagctaac ttaaccacgg aagggcccca
     2101 gcgtgggtta ttcaataatt gctcaagcca cttttgatta agttgttctt tttcttcttt
     2161 taaccctttt ttgactttgg attcatcttc catagccgct tatcttcaat ctgattctat
     2221 attaactagt atagaacgta taaagcacac tacgatattt ttgtaatcat gaagaactta
     2281 agcctgcaag tatttcttgt ctaaaaggca aaattagatc aattaggtta taatataatc
     2341 aaaaacaatc gctattaagc ggaatgatga ttaatttttt tctaagtaga actcatcagt
     2401 ccattcgaca aaaggggcag cttatgcatc cagagattga aaaggcacaa agagagatta
     2461 ctgaggcttt taacgccaaa cctaaaaatg gaatagaaaa aataaaagaa atatgtgagg
     2521 aatataaaat ttctccaaat gaagagattg ccgctttctt tcatcaacaa agaaaaaatt
     2581 tagatttaga agcggttggc gattatttga gtgggccaga agcagaaaac cagcaagtat
     2641 taaaagcttt taccagtcaa atggatttca atggacagag ttttgttgag ggtttaagaa
     2701 cctttttgaa aacatttaaa ttaccaggcg aagctcaaaa aattgacagg ttagtgcaaa
     2761 gctttagtgg tgcctattcc cagcaaaatc ctgatgtggt ttccaatgcg gatgctgcct
     2821 atcttttggc atttcaaact atcatgctta atactgattt acataaccca agtatccctg
     2881 agaaaaataa aatgactatg gatggactca aaagaaatct cagaggaggt aataatggtg
     2941 gagattttga tgccaaattt ttagaggaac tatacagtga gattaaagcc aaaccttttg
     3001 agttgaattt cgttaaaacc agcccaggat atgaacttaa ttcaaccact ctcaataaag
     3061 actctacctt taaaaagctg gattcattct tgcattcaac ggatgtaaac atcaataccg
     3121 tttttccagg aataggtgat aatgttaaaa caactgtaga tcaaccaaaa tcatggttaa
     3181 gtttttttac cggatataag ggaacaataa cgttaacgga taagcaaacg tccgcacaag
     3241 ctactatcca ggtttatact cctaatatac tttcaaaatg gctatttgga gaacaaccga
     3301 aagtaataat acaacctgga caaaccaaag agtcaatcga tttggcagca aaagttgcag
     3361 ctggtttttc atcgccagta aaaaatttta aagccacgta tgactatgag gtaggtgatt
     3421 taataaaagc atatgataac cagaaaaaac tcatttcgac aaataaaaat acattattta
     3481 aggggagtat gttcagaaaa ccaaatgctg aacaacaaga aacttcaaaa tctgcaacaa
     3541 gaacgcttaa tactgactat gatataaacg aggatacagt gactagaaaa gatgaaagta
     3601 gatcgagtta taagaaataa taatttataa aagattagct taatgtcaaa ttgattttca
     3661 taactgagag aattgaaatg gacgcactac aaacacagca aaaatgaagg caaaagtaaa
     3721 tgttttaaga gttccggcca ataaaagaga aaccaacgta gtagcagttt atgtggtatt
     3781 agatgtagta gttcaaacct aatctggcac cattgcttga aaaaatgaga gctcccggtt
     3841 ttaatgaaag tgcaaacctt aaacaaaacc aagaaaaact gatagtaccc taaaacaagt
     3901 ttacctatga atatcataac taacactaat aatatatttt acttagcaaa ataagtatgg
     3961 aatctgtatt ttatttaacc acttaagttt caaaatgcgt ctcatagaca tacacaagtt
     4021 agtaaaggta aattaaccga caggtgctta tgatgcctta ctgtaatttt tgaatgtaac
     4081 ctaaacttta agtaatcagg acattgggga gcttcatgac agacattgcg gttattatca
     4141 ataatcgagc taaaaatgca caccgaatgg aagattattt atccgaactc agagcgcatg
     4201 gcattgatta tcaattatac aaagcagacc ctcaacaatt ggaaaagaat attcagcatt
     4261 gttgctcaaa atactcttta ctattaatcg ggggtggaga cggcactgtt cggagtgctg
     4321 ctcaatggtg cagcaatacg tctgttattc ttggtgtgct tcctttagga actatgaacc
     4381 atttttccaa agagttaaat ttaccttcaa ctactgagga attaatagca gcaatcgtgg
     4441 aaaaaacaac gaccacaatt gacgtagctg aagtaaatgg ccatatcttt attaacaact
     4501 catctatcgg cttctactca aggcttgcaa aaaaccgtga ttattataca aatttttata
     4561 ataaatggct tacttacatt cccagtttta ttcgggcttt gtcataccac ccctcttatg
     4621 ccttattaat aaaaaataag gaacttgata tatcaatacg cacttccttt tttatggtaa
     4681 gcaacaattg ctatacctac gaatttcccc tcaaatttac ccgggaagtt ttcaaaatga
     4741 gcagctgggc atttattatt acaaacgcag caaattgcgt ttattcaaat ttttaattga
     4801 tttcttaaca gataagga
//
LOCUS       DAXLDT010000206         4810 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXLDT010000206 DAXLDT010000000
VERSION     DAXLDT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.207"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            76..642
                     /gene="lirB"
                     /locus_tag="ACF2SP_002829"
     CDS             76..642
                     /gene="lirB"
                     /locus_tag="ACF2SP_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system effector LirB"
                     /protein_id="HFD2360595.1"
                     /translation="MNKIICLLSNLLLTVGIMSAHAETAIIKTSEGNITCELFTKEAP
                     NTVANFVGLATGTKEFKDVKTGEMVKRPFYNGLIFHRVIAGFMIQGGDPLGNGTGGPG
                     YTFDNENTNASFNKPGVLAMANAGPNTNGSQFFITVAPTPELQGNYNVFGQVISGQEV
                     VDKISKMPTDPQDKPIKPVVIENITIQK"
     gene            complement(823..2379)
                     /locus_tag="ACF2SP_002830"
     CDS             complement(823..2379)
                     /locus_tag="ACF2SP_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2360596.1"
                     /translation="MIKDILNTIASLIEENAEIFAGECEKQLLQKVKVDLESFKPINF
                     YALDAEKQIADLLKLIESYCIEMSRKLDELNHQYPISLVNEKLISKIENILTELKNKQ
                     NKITQLRDKIPEGILDIKRALYIAESSFLSESQAEETEITSKFKRIKKFMSNLDREYL
                     DLIKKGGLLNSILLDTTTLYWIKLREYSVLLIECCFSLQKSHQLTEEGRLALRCFDRA
                     IQKMPSQKHIINALKILSKPGNKTEAIERIGFFSPNGREACIQLSRWGKKFFYIPRSE
                     EMYHFDYDPKEDLNDTGGNCFGESMMFIHFLSKGVIRWLCPEAGLINFQLDQTRNLKF
                     KKTILGEGETVVSESSPHDSIQWEDMMPVLLDNPYFKPGSLCGVIFSMNDYTKAHREF
                     TAGHIIVLAKLDTKLNPYRYIVYEKDFGAFGLSDDESLEYLINKKILPLYRGLNYSKV
                     KLVKYGKASTETYDLLNEIKPQTGVPQNELTASDSIHTYFDNAFFKLSKETNKENEMN
                     QANAPWSRCN"
     gene            complement(2715..2942)
                     /locus_tag="ACF2SP_002831"
     CDS             complement(2715..2942)
                     /locus_tag="ACF2SP_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360597.1"
                     /translation="MFFKRSNPHITPQDLQKVIQNLNAQRELTERQLKEGSISQKTGQ
                     EEMQRLSSLIGAYQNNLMAALDDQQNTNCPK"
     gene            3204..3764
                     /gene="psrA"
                     /locus_tag="ACF2SP_002832"
     CDS             3204..3764
                     /gene="psrA"
                     /locus_tag="ACF2SP_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor PsrA"
                     /protein_id="HFD2360598.1"
                     /translation="MNISNTKERILAVAEALIQKDGYNAFSFKDIATAINIKTASIHY
                     HFPSKEDLGVAVISWHADKIAAVLSDISNNSSLSAKEKIQKFFDAILTITYNAENKMC
                     LGGMFASDFQSLPVSIQNQAKKFFEIIVEWLKGVLEANGYDKESSLSIAKQIISLIEG
                     GLLLARLYGDETFLEGVRRFIDQTIK"
     gene            3823..4572
                     /locus_tag="ACF2SP_002833"
     CDS             3823..4572
                     /locus_tag="ACF2SP_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360599.1"
                     /translation="MSKVKAKIIAFTTVMLFLTSINIHAKGETTMNQYHQTNEATMIT
                     MKSKLPNSSEFETFLKSGGQLVKQTEPDTKVWFALKGNDNSLMIFDAFYDSKGREAHF
                     AGQVANALKNNSEQLVLQGWENGVLKNIVNSQVLASKLPARSVPVTVANYIEFTANDG
                     KSAQLAALLSNAAEIVDKTEPQTAYWFALQLNDNTFAIFDAFSDESGQKAHFSGKVAL
                     SLKNHSELLIKNGWEKGVLPNIQNLKVITNI"
     gene            complement(4629..>4810)
                     /locus_tag="ACF2SP_002834"
     CDS             complement(4629..>4810)
                     /locus_tag="ACF2SP_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360600.1"
                     /translation="CNCDPTDNKMCASQNDLEHPCPSVCASPAPGMAPSFTACPVSRV
                     TDPVTGVTRWVNMCN"
BASE COUNT         1551 a          878 c          804 g         1577 t
ORIGIN      
        1 agtttcattt aaaaaccatt ttaatacgac ataatactgt tatactggtc tgagtcattc
       61 tatggagaac ttcttatgaa taaaattatt tgtttactca gcaacttatt gttaacagta
      121 ggcatcatgt cggcacatgc ggaaactgca attattaaaa cctcagaagg caacataacg
      181 tgtgaattat ttacaaaaga agctcccaat actgttgcaa attttgtagg gctggcaacc
      241 ggaacaaaag aatttaaaga tgtaaaaact ggtgaaatgg ttaagcgtcc tttttacaat
      301 ggcctgattt ttcatcgtgt gattgcaggc tttatgattc aaggtggcga cccgcttgga
      361 aatgggaccg gtggcccagg ttacacattt gataatgaaa atactaatgc cagttttaac
      421 aaacctggcg tacttgcaat ggctaatgca ggccccaata ccaatggcag ccaatttttt
      481 attaccgttg cgccaacacc tgaattacaa ggcaactaca atgtttttgg tcaagtaatt
      541 tccggacaag aggttgttga caagattagc aagatgccta ctgatcctca agataagcca
      601 ataaagcctg tagtcattga aaacatcacc attcaaaaat aaatacgcag ccagttctag
      661 tgataatcgg tattgcaaga taggatttct atattaccat acatcaaaaa tttagctttc
      721 aagcaattcg aattaatgaa ctggctcctc tacaaaacca gctttgacaa gacattttat
      781 ttattctctg aggttttagc ttttcctcat taaccttttg attcaattac atcgagacca
      841 gggtgcatta gcttgattca tttcattctc tttattagtt tccttagata acttaaaaaa
      901 tgcattatca aaataggtat gtatagaatc actggctgtc aactcgtttt gtggaacacc
      961 tgtctgtggt ttaatttcat ttaaaagatc ataggtctca gttgaggcct tgccatattt
     1021 aaccaattta acttttgaat aattcaaacc tctatagagt ggcaatattt ttttattgat
     1081 tagatattcc aaagactcat catctgataa tccaaatgct ccaaaatctt tttcatagac
     1141 aatgtatctg tatggattta acttggtatc caacttggca agcactataa tgtgccctgc
     1201 ggtaaactct ctatgtgctt ttgtgtaatc attcatcgaa aatatgacgc cacaaagact
     1261 gcctggttta aagtaagggt tatccaaaag cacaggcatc atatcttccc attgtataga
     1321 gtcatggggg ctactctccg ataccaccgt ttccccttca cctagaattg tttttttaaa
     1381 ctttaagttt cttgtctgat ctaactgaaa attaattaac cctgcttcgg gacacaacca
     1441 tctaattact cccttagata aaaaatgaat aaacatcatt gattcaccaa aacaattacc
     1501 gccagtatcg ttcaaatcct ctttaggatc ataatcaaaa tgatacattt cctcacttcg
     1561 aggtatgtaa aaaaattttt tcccccaacg acttagttgg atacaggcct ctctaccatt
     1621 aggtgaaaaa aaacctattc tttcaatcgc ttctgtcttg ttacctggct tgctcaatat
     1681 ttttaatgca ttaatgatgt gtttctgtga tggcattttt tgaatagccc tatcaaaaca
     1741 acgaagcgct aatcgtccct cttcagtgag ttgatgcgat ttttgtaagc taaagcaaca
     1801 ctcaattagt aacacagaat attcacgtaa cttaatccaa tatagagttg tggtatccag
     1861 taaaatagag tttagtaatc cccctttttt aattaaatca agatactccc tatccaaatt
     1921 actcatgaat tttttaatgc gcttaaactt tgatgtaatt tctgtttcct cagcttgtga
     1981 ttcgctaaga aaggaacttt ctgcaatata taatgctcgt ttgatatcaa gtatgccctc
     2041 tggaattttg tctcgtaact gggttatttt gttttgtttg ttctttaatt cagttaaaat
     2101 attttctatt ttgctaatca atttttcatt gactaaagag attggatatt gatgatttaa
     2161 ttcatctaat tttctgctca tttcaatgca atagctttct attagtttaa gcaagtctgc
     2221 tatttgtttt tctgcatcca atgcataaaa attaattggc ttaaacgatt caagatcaac
     2281 tttaaccttt tgtaaaagtt gtttttcaca ttcaccggca aatatctctg cgttttcctc
     2341 aataagactg gctatggtat tgagaatgtc ctttatcatt taatactctc ttactcgaca
     2401 gaatgggctt tgattgaata gaaataccta tacagattga ataaagcaag tactttctgg
     2461 gttaattatt cataaatttc aataatctat tttatttcca atctcttaaa tagactcctg
     2521 aatattattc aaagcaaagc actagtgagc atgcggatga attgataggg taggaaataa
     2581 catccctgtt ttcttagtgt ataggatggc acagagctct caaaattgta tcaacacata
     2641 aatgaccatt cagattatga gttgattatg ccattcatca aaatgactgc ccttttatac
     2701 ctcaaaaaga ttagctactt agggcaatta gtgttttgtt gatcgtctaa tgctgccatc
     2761 agattattct gatatgctcc aatcaggctt gataaacgtt gcatctcttc ttgccctgtt
     2821 ttttgcgata tagacccctc tttcaattga cgctctgtta attcacgttg cgcatttaaa
     2881 ttttgaatca ctttttgtaa gtcttgtggt gttatgtgag ggtttgatct tttaaaaaac
     2941 ataatgacct ctattgtgaa ctgatgcaca tatattacct cccgtttttt ttgagatcaa
     3001 taaagacata aagtctttaa ggccaattga catggcgatg taccaagata ttgatccgat
     3061 caatcaaccc aataaagatc ggaatgaatg aatgagcagt ccaccatcca cttccattta
     3121 gataaacaag tgttatttta atttgatata gaaaactata aaaagtttga caatctatct
     3181 atgagtaggt aaaattcata cctatgaata taagtaatac gaaagaacgg atattagcag
     3241 ttgcagaagc attgatccaa aaagatggat ataacgcctt tagtttcaaa gatattgcaa
     3301 cagccatcaa tattaaaact gccagcatcc attatcattt tccttcaaaa gaagatcttg
     3361 gtgtggcagt tatatcctgg catgccgata aaattgctgc tgtgttatct gacataagca
     3421 ataattcgtc gttatcagcc aaggaaaaaa ttcaaaaatt ctttgatgcc attttaacaa
     3481 tcacctataa cgctgaaaac aaaatgtgtc tcggaggtat gttcgcttct gattttcaat
     3541 cattacccgt ttcaattcaa aatcaggcaa aaaaattttt tgagattatc gttgaatggc
     3601 ttaaaggagt tcttgaagca aatggttatg ataaagaatc ctcattatct atcgcaaaac
     3661 aaattatttc cttgattgaa ggcgggttat tattagcaag attatacgga gatgaaacgt
     3721 tcctggaagg agttcggcgc tttattgatc aaacaataaa gtgatttttt ttaacatcca
     3781 aaactatcta ttagtagata gaatattatg gggaaatttt atatgagtaa agtcaaagca
     3841 aaaattatag cttttacaac agtaatgttg tttttaacat cgatcaatat acatgcaaaa
     3901 ggagagacaa ctatgaatca atatcatcaa acaaatgaag caaccatgat aacaatgaaa
     3961 agcaaattac caaattcctc tgaatttgaa acatttttga aatctggcgg tcaattggta
     4021 aagcaaactg agccagatac aaaagtatgg tttgccctta agggtaatga taattccttg
     4081 atgatctttg atgcatttta tgactctaaa ggaagagaag cccattttgc cggtcaagtt
     4141 gccaatgcac ttaaaaacaa ttctgaacaa ttggttcttc aaggttggga aaatggcgta
     4201 ttaaaaaata ttgtaaacag tcaagtgctc gcctctaaat taccagcaag atcagttcca
     4261 gtcactgttg ccaattacat tgagttcact gccaacgatg gcaaatcagc gcaacttgcc
     4321 gcgttattaa gtaatgccgc tgagattgtt gataaaactg agccgcaaac agcctactgg
     4381 tttgctttgc agcttaatga caacaccttt gcaatttttg atgctttctc tgatgagtct
     4441 ggtcaaaaag ctcatttttc aggaaaagtt gcactgtcct taaaaaatca ctcagaactc
     4501 ttgattaaaa atggatggga aaaaggtgtt ttacctaaca ttcaaaacct gaaagtgata
     4561 accaacatat aaccagtcag gtaaattaaa gataaaattc ttccctgaaa ataatggatt
     4621 atttgatatt agttacacat attgacccat ctggtaactc ctgtgactgg atcagtaacc
     4681 cttgatacag ggcatgctgt aaacgaaggc gccataccag gtgctggtga agcacaaact
     4741 gaagggcaag gatgctccag atcattctga ctggcacaca tcttattgtc tgtcgggtca
     4801 cagttgcatt
//
LOCUS       DAXLDT010000207         4781 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXLDT010000207 DAXLDT010000000
VERSION     DAXLDT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.208"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..614)
                     /locus_tag="ACF2SP_002835"
     CDS             complement(<1..614)
                     /locus_tag="ACF2SP_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360601.1"
                     /translation="MTVQKYFPWFNEAWQSYSLCRSSLWWPLSYLILTPLAVFIVFGL
                     IIPLGLNQLGWISFDHALINYPYLLLIAGLLYLALFPFLDGLYQMIKAHLDGKPINMN
                     TEDIDFINPGTSKVMGMSMGPILLTIIIMEIAGSFFKPFRALVLLAYVLAIFTPILSV
                     NNPESPMKKGLESLQFALNNKKLVTKVWGLRLLILLSLLLPWVI"
     gene            complement(792..2303)
                     /locus_tag="ACF2SP_002836"
     CDS             complement(792..2303)
                     /locus_tag="ACF2SP_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFD2360602.1"
                     /translation="MYMDFGSDNMALSKEIQNKSLRSLIQKYARQLNETDKKLIELIN
                     NLAAADDIPLYAQNQNVINLLIKTIKDSQSEHSPDLEGTSYFIYGLITAEMEAQSEIN
                     RQRHLMQGRFDETALYLRTERDQMQDHDMTLLMPKSQGRIVVMAVLNRYDTHSANAII
                     EALASDVFNPEVHHIMIPVGPGHWRGVYLSKPQGGISDTVYDLELFDPYGPAGAAVLD
                     DYVLDLLNQCGVPNELVNIRHTGPKHPQGDAYSCGDFTCAYSHKKMKEFGAPEGSYNP
                     ILIDTLDNLGNEDNVLRMATREETRALVDKDRRRPLINPAIHSMRGADKEKPVSSIGK
                     PKAGTQGISFEIENANKSKKATIRQTVKESELSPVMRTGFFIGGGTFISGGIGSIIGG
                     LIGFFLLPGLGVLIGAIAGAFIGAGAGLLISGIVASAFWNSTPLNIIKTHESDTEIQG
                     VDLKSDSAGKPDKECSEKDEKPIHSPPLFFTPATQERNTNDMADELSSSGPKT"
     gene            complement(2597..3100)
                     /locus_tag="ACF2SP_002837"
     CDS             complement(2597..3100)
                     /locus_tag="ACF2SP_002837"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360603.1"
                     /translation="MLFFSSAMPKHKLTFTKLSNCMKYLDLAATWAEEEWGYIRNKGV
                     EFRKTILSDLKEHTYIGTFNGQPIAMFVLFDKDMSSEFNYGRLKPPVVSELMYVYIDE
                     PYRGLGFGKQIIDEAKQLASYSKAEYIMLDTLKPGLNRFYEKSGGKVIAENQLFLHPT
                     DVLTIKI"
     gene            complement(3161..4642)
                     /locus_tag="ACF2SP_002838"
     CDS             complement(3161..4642)
                     /locus_tag="ACF2SP_002838"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFD2360604.1"
                     /translation="MNAYQQLEQHFKKYYDFENLAAIVSWDEATMMPAGGGQARAEAL
                     ATLSAVQHEWLTNKKVGDWIKQAKDLPSLSLWQRRNIYWIDKQYQQATCIPAQLIEEF
                     TNESLICVQAWRELRAKNDWRSFKPYFKKLFEKVKMIADIKSQRFNKPCLDVLIDEYS
                     PDLDCKAITPVFDVLKQEIPAIIPNVIEKQKRRDIKVISGDFSAEKQKQFGIEIMSRL
                     GFDFNRGRLDVSHHPFCGGISDDVRMTTRYSEQEFISSLMAITHETGHALYEQGLLKE
                     WSRQPVGHSLGMSVHESQSLLIEMQICRSLEFISFLVPIAKKYFKEAQNITEENLYFH
                     CTKVQPDLIRVEADEVTYPLHVILRYEIEQLLFSSEITIDDLPEVWDNFMRKYLGLST
                     KGNDKNGVMQDVHWPSGSFGYFPAYTFGALIAAQLFSAAKKAIPDINDQIKSGDFQSL
                     LIWLRENVHQKAKLFSYQELLCQATGEELNPQYYLAHIRQRYL"
BASE COUNT         1487 a          990 c          796 g         1508 t
ORIGIN      
        1 ataataaccc agggtaagag caaggacaga agaatcaaaa gacgtaaacc ccaaacctta
       61 gtaaccaatt ttttattatt aagagcaaat tgcaaggact cgagtccctt tttcataggt
      121 gattcaggat tatttacact taaaataggc gtaaaaatag ccaatacata agctaacagc
      181 accaatgctc ggaaaggttt aaagaaagag ccagcgatct ccatgataat aatagtcaat
      241 aaaatggggc ccatagacat gcccattact ttggaagtac cggggttaat aaaatcgata
      301 tcctctgtat tcatattgat aggcttgcca tccaggtggg ctttaatcat ctggtatagt
      361 ccatcaagaa aagggaaaag agccaaataa agaagccctg ctattagcaa aagataagga
      421 taattaatca atgcatggtc aaaactaatc caacccaatt gatttaaccc taatgggata
      481 attaatccaa aaacaataaa gacggcaagt ggtgttaata taagataact taaaggccac
      541 catagcgaag agcggcaaag agagtaagat tgccatgcct cattaaacca ggggaagtat
      601 ttttgcacag tcatgaaatg tcggacgcta attatattaa atgatttata ttagcacgtt
      661 aattccatag ttgaaagaat gctcacaaat gctgaattga catattgaaa ggtatgttaa
      721 caattcatcc tcaccgccta cgtaccgctg cttgccagcg gtatccataa aataaattta
      781 tgattgtttt tctatgtttt aggaccgctt gaactcaact catcggccat gtcatttgtg
      841 tttctctctt gcgtagcggg agtgaaaaat aaaggaggac tgtggatagg cttctcgtcc
      901 ttttccgaac attctttatc aggtttacct gcagagtcgg attttaaatc gactccttga
      961 atttcagtgt cactctcatg ggtttttata atatttaatg gagtactatt ccaaaatgca
     1021 ctggctacaa tacctgatat caacaaacct gctcctgcac caataaaagc tcctgctatc
     1081 gctcctatta atacacccag tcctggcaaa aggaaaaaac ctatgagccc accaataata
     1141 gaccctattc ctcctgagat gaatgtaccc ccacctataa aaaaacctgt tcgcataacc
     1201 ggtgataatt cactttcttt aacagtttgc cgaatagttg ctttttttga tttattagca
     1261 ttttcaattt caaaggatat cccttgtgtt cctgctttag gtttgccaat agacgatact
     1321 ggcttttctt tatctgcccc ccgcatagaa tggatagcag gattaatgag cggtcttctt
     1381 ctgtctttat caacaagcgc tcgcgtttct tcgcgtgttg ccatgcgaag tacattatct
     1441 tcattaccta aattatccag tgtatcgata agaattggat tataactacc ttctggagct
     1501 ccaaattctt tcattttctt atggctataa gcacaagtaa aatccccaca tgagtatgca
     1561 tcaccttgcg gatgtttcgg acccgtatgc cgtatattca caagctcgtt gggtacgcca
     1621 cactggttta ataaatcaag tacatagtca tcaagtactg cagcgcctgc aggcccataa
     1681 ggatcaaata attcaagatc ataaactgta tcgctaatgc ccccttgggg cttgcttaaa
     1741 taaactcctc gccaatgtcc aggtccaaca ggtatcataa tgtgatgaac ttcgggattg
     1801 aatacatcac ttgccaaagc ttctataatc gcattagcag aatgagtgtc ataacgattg
     1861 agaactgcca tcacaacgat acggccttga gattttggca tgagtagagt catgtcatga
     1921 tcttgcattt gatcacgctc tgttcttaaa tacaatgctg tttcatcaaa acgtccctgc
     1981 ataagatgtc tttggcggtt tatttcagat tgagcctcca tttctgcagt gatcaatcca
     2041 taaatgaaat aggaagttcc ctccaaatcc ggactgtgtt cagactggct gtctttaatg
     2101 gttttaatta acaaattgat aacattttgg ttttgggcat acaaaggaat atcatctgct
     2161 gcagcgagat tattaataag ttctatgagc ttcttgtctg tttcattaag ttgtctggca
     2221 tatttttgga ttaatgatct taaactttta ttctgaattt ctttacttaa tgccatatta
     2281 tcgctcccaa aatccatata cagatgctat ataaaacaat taagaaaaaa tgaaacctct
     2341 tccctcctct ctaataatgt atgcccctat ctgaagcagt tcgacaatgc ttttggctct
     2401 aaattagacc ttttgcctaa cctgtctctt ttgttttagg gcaagaggtc taatgaaata
     2461 aaaaataatt aaccgaaaag aaatttactc tcatgattaa ttattgcacc cattgaatat
     2521 atgattactt aaacacgata acatgctcat gtctacataa aaaagatcat tacttacccg
     2581 tgatagagat cacagcttat atctttattg tcaatacatc agttggatgc aaaaacaatt
     2641 gattttctgc aatcactttc cccccactct tttcataaaa tcgattcaag ccaggtttta
     2701 aggtatctaa cataatgtat tccgcttttg aatagcttgc taattgtttc gcttcatcaa
     2761 taatttgctt tccaaacccc agtcctctgt atggttcatc aatatagaca tacatcaact
     2821 cacttactac cgggggtttc aatcttccat agttaaattc cgaactcatg tctttgtcga
     2881 ataaaacgaa catggcaatg ggttgtccat taaaagttcc tatataagta tgttctttca
     2941 aatcagacag gattgtttta cgaaactcta cccctttatt acgaatatat ccccattctt
     3001 cctctgccca tgttgcggca agatccaaat atttcatgca attagaaagt ttagtaaaag
     3061 tgagtttatg tttgggcatt gctgaactaa aaaataacat ctgataaccc tcttgattaa
     3121 aatgacacaa cctgcaaatt ttattcaaga ccatcctgtt ttataaatac ctctgtctga
     3181 tatgagccaa gtaatattgc ggatttaact cttcgcctgt tgcttgacac aataactctt
     3241 ggtaagaaaa taactttgct ttttgatgaa cattttctct aagccaaatt aacaacgatt
     3301 gaaagtcacc tgatttgatt tgatcattga tatcagggat tgcttttttg gccgcagaaa
     3361 acaattgagc ggcaattaat gcaccaaacg tataggcagg aaaatagcca aaacttccag
     3421 aaggccaatg cacatcttgc atcacaccat ttttgtcgtt cccttttgtt gataatccta
     3481 aatattttct catgaagttg tcccagacct ctggtaaatc atcaatagta atctctgaag
     3541 aaaacaaaag ttgttcgatt tcgtaacgca gtattacatg tagtggataa gtgacttcgt
     3601 ctgcctcaac acgaattaaa tcgggttgta ctttcgtaca atgaaaataa agattttctt
     3661 cagttatatt ttgagcttct ttaaaatatt ttttcgcgat aggaaccaaa aaacttataa
     3721 actccaaact tcgacaaatt tgcatctcaa taagcaacga ttggctctca tgcacagaca
     3781 ttcctaatga atggccaaca ggttgccgac tccattcttt tagcaagcct tgctcataca
     3841 aggcatgacc tgtttcatgg gtaattgcca ttaaggaaga tatgaattcc tgctcgctat
     3901 atcgggttgt cattcgaaca tcatctgaaa taccaccaca aaacggatgg tggctaacat
     3961 ctaatcggcc gcgattaaag tcaaacccca atcgagacat gatttcaatc ccaaattgct
     4021 tttgtttttc cgcagaaaaa tcacctgaaa tcactttaat atctcgcctt ttttgttttt
     4081 cgataacgtt gggaataata gctggtattt cttgtttcaa gacatcaaaa accggggtga
     4141 tggccttgca atccaaatcc ggggagtatt cgtcgattaa gacatccaaa caaggcttat
     4201 taaatctttg tgatttaata tctgcaatca tttttacttt ttcaaataac tttttaaagt
     4261 acggtttaaa tgagcgccaa tcatttttag ctcgtaattc ccgccatgcc tgaacacata
     4321 tcaaagattc attcgtaaac tcttctatca gttgcgctgg aatacaggtt gcctgctgat
     4381 attgcttatc aatccaatag atatttcttc gctgccataa tgataaactg ggtaaatctt
     4441 ttgcctgctt tatccagtca cctactttct tattggttaa ccattcatgt tgtacagcac
     4501 ttaatgtggc caacgcttct gcacgcgcct gcccaccccc ggctggcatc atcgttgctt
     4561 catcccagga aacaatggct gctaaatttt caaaatcgta atatttttta aaatgttgtt
     4621 ctaattgttg gtaggcattc atgaaagact cttggcttat actttaaaaa tatgggttcc
     4681 actcagatct gacagctttc aaagcaccac atacagaata acatagttaa aatcctttca
     4741 tgaaatttaa aaactgactc atgaaaaaat attatagacc a
//
LOCUS       DAXLDT010000208         4770 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXLDT010000208 DAXLDT010000000
VERSION     DAXLDT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.209"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1023)
                     /locus_tag="ACF2SP_002839"
     CDS             complement(<1..1023)
                     /locus_tag="ACF2SP_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFD2360605.1"
                     /translation="MITNNIRFNWIIFILLIGLLNLANINELHADETGEQSQFNLESY
                     SPQTKKLCQTVAHISVPSQNQLDEASKASLKNCDALDLYYGFNAAPDYDKAFQCALMN
                     KDYNVLVMAYANGRGVTFNPDLAMHFACMMEDAAPAEMDSRIAHLAQIKEGNKKTSFD
                     ICDDITSGYMMGWCSSIDQRLEDVKRNKKINDLVSQWTAQEQLLYQQVRKTAEVYIRD
                     HSMNEIDLSGTARSAFAINAQLNLNQRLFELLQKVNRCETPLMTIKQYEEMDKQLNNI
                     YKKLMADTSSFQYTTVTKEGIKKTEIAWIQYRDAWIQLARVKCPKISVESWQVLLIQD
                     RIKLLNE"
     gene            complement(1050..1544)
                     /locus_tag="ACF2SP_002840"
     CDS             complement(1050..1544)
                     /locus_tag="ACF2SP_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2434 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360606.1"
                     /translation="MTIMMNKDKHSLLAIAAAEVPPRTKPSIYPEPFASMMTGRQKHA
                     LGDFFGIKNFGVNLTRLAPGAQSALLHKHKLQEEFVFILKGQPTLVTETADIQLHPGM
                     CAGFTPDGTAHQLVNRTADEVVYLEIGDRTQGDEVNYPADDLAAAFGNDGQWHFMHKN
                     GMPY"
     gene            complement(1811..3607)
                     /locus_tag="ACF2SP_002841"
     CDS             complement(1811..3607)
                     /locus_tag="ACF2SP_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360607.1"
                     /translation="MEIYMAPLFPEKNAPLTIKQGPVDDCYLLVALDCIFNSGDEARQ
                     LLASKFTQTPLGLTLRIKRNNQSTRLDSQKMRGKYIHIYDRNTDEDVFFIGQQKLKEI
                     DNSKKGVETNSLAIKVLERISSYYYTGDWDHTNESGSLTAHSIRQRHMETSTRFVGKL
                     LGIHAEDTEEMDAIIKLKLMNPKEAIYISLAYGEPDANGKIHDRHALRIDKVVPKING
                     GYDFIFVNPWDNQKQETFSLEELEKRDCRFCTFSLNQQRHEITRILLKNPIGQGQYIF
                     ANPELFTLLLQMQKLSLLSDAKNIQACINLHKQMPYLPSLYNSLTSDEQKKLIQGMVG
                     TKADKEKFIKHLIINAPRLDLIKTIFQNEPPENKTENMIVDLALEAKKKNSPARIVFE
                     APEFFTLIMNRAIHHKATIRGCSQEQAKKLIESKMINFYFDNLGTDSLTRNARLCELF
                     AAKIFTKTSIETWFAARLSLTNVDLSQALRTLGFSDQRSGVMSAPVLKNQTALVAGQV
                     GFFPIHKSSPAPVTNKSPAPTQELTAKKSSVPTLPSIVKKPSTPLSEPIGKKLSTPIP
                     ALLVNGLSIYTPVKPSVKTLPPLKGVRLTSLP"
     gene            complement(3773..4666)
                     /locus_tag="ACF2SP_002842"
     CDS             complement(3773..4666)
                     /locus_tag="ACF2SP_002842"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFD2360608.1"
                     /translation="MKNLLFLVLVILTNSLMAKENLVFAVIGDYGANSAAEAKVASVL
                     KSKNPQFILTLGDNNYTQGCWKTIDKNVGKYYHEYIGNYQGKYGKGSDINRFFPTLGN
                     HDWLARKTCLYQGTLPYFSYFTLPGNQSYYDFVRGPIHFFALDSDSHEPDGSKEGSKQ
                     YQWLTEQVQQSKAPFKIVYFHHAPLSSGKHGSNTRMQWNFASMGIDVVMSGHDHHYER
                     IERNGIVYFVNGVGGAELYSYKKWVEGSKFFYSKHHGFMLITALEHAMKIQFINENDE
                     IKDEIVIQEKKQGAGEHQNLR"
BASE COUNT         1390 a          870 c          909 g         1601 t
ORIGIN      
        1 ttcattgagc aatttaatgc ggtcttgaat caaaagcact tgccagcttt ccacgctaat
       61 cttggggcat ttcacacgag ccagttgaat ccaggcatcc ctatattgaa tccaggctat
      121 ctcggttttt ttaatgcctt ctttggtaac cgttgtgtac tgaaaggatg aagtatctgc
      181 catcaatttt ttatagatat tattcaattg cttatccatt tcctcatatt gttttattgt
      241 cataagagga gtttcgcatc gatttacttt ttgtaacagt tcaaacaagc gttgattcag
      301 attcaattgt gcattgatgg caaatgcgct tctggcagtt cctgataaat caatttcatt
      361 catcgaatga tcacggatat acacttcagc agtttttcta acctgctgat ataataattg
      421 ttcttgagca gtccactgac tgactaagtc attaattttt ttattgcgtt ttacatcttc
      481 caggcgctga tcgatagaag agcaccaacc catcatatac ccactggtaa tgtcatcaca
      541 aatatcaaaa gaagttttct tattaccctc ttttatctgt gcgagatggg caatacggct
      601 atccatttcc gctggagcgg catcctccat catgcaggca aaatgcatgg caagatcagg
      661 attaaatgtt actcctctac cattggcgta agccataacc aggacattat aatctttatt
      721 cattaatgcg cattgaaagg ctttgtcata atcgggggcg gcattaaaac cataatagag
      781 atcaagagca tcgcagtttt ttagtgaggc tttactggcc tcatcaagct ggttttggct
      841 tggtactgat atatgagcta ctgtttggca taatttcttt gtttggggag aataactctc
      901 taaattaaat tgactttgct ctccagtttc atctgcatga agttcattaa tattggctaa
      961 atttaataaa ccaattaata gaataaagat aatccaatta aatcttatgt tgttagttat
     1021 cataattttt tctttctaat tatttgttat taatacggca ttccattttt atgcataaaa
     1081 tgccattgcc catcattacc aaaggctgct gccagatcat cggcaggata atttacttca
     1141 tcgccctggg ttcgatctcc tatttcaaga taaaccactt catcagctgt ccgattgacc
     1201 aattgatgtg ctgtgccatc tggagtaaat ccagcacaca ttccgggatg taattgaatg
     1261 tcagcagttt cagtgactaa tgttggctgc cctttgagga taaaaacaaa ctcttcctgt
     1321 aatttatgtt tatgtagcaa cgcagattgt gctccaggag ctaatcttgt taagttgacg
     1381 ccaaaattct ttattccgaa aaaatcccct aatgcatgtt tttgacgacc agtcatcatg
     1441 gatgcaaacg gctccggata aatggaaggt ttggtgcgag ggggaacctc agctgctgct
     1501 atagcaagca atgaatgttt gtctttgttc atcatgatcg tcaatttgag ttctatcctt
     1561 gcaatattta accaatatta gcataaagta aattatcttg aatccctact ttcaggaatt
     1621 ctagattatg gatttgctac tgaatttagc gaaaccaagg caagattaat cttaggcaac
     1681 agaaagtaaa ctatagagaa aggttttaag tgagttaaat ttttctatga atctgtttcc
     1741 agatcttcgc tttaggagtt tcaggctaaa aaattaatta tcagccagca caatagatat
     1801 atcatgaact ctaggggagt gatgtaaggc gcactccttt tagaggaggg agcgttttta
     1861 cagagggttt cactggggta taaatggata atccattcac gagaagtgct ggaattggtg
     1921 tagataattt ttttccgata ggttctgaaa gcggagtaga tggttttttt actatagatg
     1981 gaagagtagg aacagatgat ttttttgcag tcagctcctg ggttggagca ggagatttat
     2041 ttgttactgg tgccggtgag gatttatgga ttgggaaaaa tccaacctgg ccagccacca
     2101 aagcggtttg attttttaga accggtgcgc tcattactcc agaacgttga tcagaaaatc
     2161 ccagagttct taatgcttga gacaaatcaa cattagtcaa agaaagtctg gcagcaaacc
     2221 aggtttcaat ggatgttttt gtgaaaatct ttgcggcaaa taattcacat agccttgcat
     2281 ttcgagtcag actatctgtt cccaggttat caaaataaaa attaatcatt ttggattcaa
     2341 tcaatttttt tgcttgttcc tgactgcatc cccgaatggt tgctttatga tgaatcgctc
     2401 gattcatgat taaagtaaaa aactccgggg cttcgaaaac aatgcgtgct ggtgaatttt
     2461 tcttttttgc ttcaagtgca aggtcaacaa tcatgttctc agttttattt tcaggaggct
     2521 cgttttgaaa aattgtttta atcaaatcca gtcgaggagc gttaattatc aggtgtttta
     2581 taaatttttc tttatcagct tttgtaccta ccattccttg aatcagtttt ttttgttcgt
     2641 cagatgttaa tgaattgtat agggagggca agtaaggcat ttgtttgtga agattaatac
     2701 acgcttgaat atttttagca tcggataaca ggcttagctt ttgcatttgc aagagcaggg
     2761 taaacaattc cgggtttgca aaaatatatt gaccttgccc tatcggattt tttaataaaa
     2821 tacgtgtgat ttcatgtctc tgttgattga gactgaaggt acaaaaacgg caatctcgtt
     2881 tttcaagttc ttctagacta aatgtttctt gcttttgatt atcccatgga ttgacgaaaa
     2941 taaaatcata accaccattt attttaggga ccactttatc tattcttaac gcatgacggt
     3001 catgaatttt tccattggca tctggttcac cataggctaa gctgatataa atggcttctt
     3061 tggggttcat taatttaagt ttgatgatcg catccatttc ttcagtatct tcagcatgaa
     3121 tacctaataa tttgccgaca aaacgggtgg aggtttccat gtgtctttgt cttatgctat
     3181 gagctgtcag acttccagat tcattggtat gatcccaatc tccggtgtaa taataggagc
     3241 tgattcgttc taaaacttta atagcgagtg aattggtttc tacccctttt tttgaattat
     3301 caatttcttt gagtttttgt tggcctataa aaaaaacatc ttcatctgta tttcgatcat
     3361 aaatgtggat atatttacca cgcattttct gagaatcaag tcgggtactc tgattatttc
     3421 gtttaatgcg gagagttagt ccaagtggag tttgtgtaaa ttttgatgct aacaattgac
     3481 gcgcttcgtc tccagaatta aaaatacaat ctaaagcaac taacaggtaa caatcatcga
     3541 ctgggccttg ttttatagta aggggagcgt ttttttctgg aaacaaaggc gccatataga
     3601 tctccaatta tacaaaaagt ttttaacctt gccattttat caaagaagta ttatgttaat
     3661 attaagaaaa gtcgaggcaa gaatggttta aacccatcat tttcttatgt tcaggactta
     3721 cgaaaacccc caaggctgag gtaaaaacat tttttaacaa agagactggg gtttatcgta
     3781 agttctgatg ttctccagcc ccctgttttt tttcttgaat gactatctca tctttaattt
     3841 cgtcattttc attgatgaat tggattttca tggcatgttc caaagctgtt atcagcataa
     3901 atccgtggtg tttagagtaa aaaaatttgc ttccttctac ccatttttta taggaataaa
     3961 gctccgctcc tccgactcca ttgacaaaat aaacaatccc gttgcgttcg atacgctcgt
     4021 aatgatgatc atggccactc ataaccacat caatacccat agaggcaaaa ttccattgca
     4081 tacgggtatt ggagccatgt ttaccagaac tcaacggagc atgatgaaaa tagactattt
     4141 taaaaggggc ttttgattgt tgtacttgtt cagttaacca ttgatattgt tttgaacctt
     4201 ccttgcttcc atctggttcg tgagagtcac tgtccaaggc aaaaaaatga attgggccgc
     4261 gaacaaaatc ataataactt tggtttccag gaagggtaaa ataactgaaa taaggtaatg
     4321 taccctgata taaacaggtc tttcgggcaa gccaatcatg attacccaga gtagggaaaa
     4381 agcgattgat atcagaacct ttcccgtatt ttccctgata attgcctatg tattcgtgat
     4441 aatatttacc cacattctta tcaatggttt tccagcaacc ctgagtgtaa ttattatctc
     4501 caagtgtaag gataaactgg ggattttttg acttgagaac gctggctact ttggcttccg
     4561 ctgcagaatt ggcaccatag tctccaatga cagcaaaaac caggttttcc ttggccatca
     4621 gcgaattagt caggataact aaaacaagaa acaacaaatt tttcaaagaa gatccctgtg
     4681 cttgatggag cgaagccgct atggtaagag atctcttttt aataaatcaa tagaattgaa
     4741 agtacaaaat gattaagtca atttattttt
//
LOCUS       DAXLDT010000209         4760 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXLDT010000209 DAXLDT010000000
VERSION     DAXLDT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.210"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            281..1432
                     /locus_tag="ACF2SP_002843"
     CDS             281..1432
                     /locus_tag="ACF2SP_002843"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFD2360609.1"
                     /translation="MRLLSFKKSKIVSIGTELEFQIIDCSSLSLVSRSKELMRALKDM
                     RYRDQIKPEITQSMIEINSSIHQSAKEMYDELLELQKILVETAASIDIAFCGGGTHPF
                     QQWTMQKIFPSKRFKKKFNQYRYLSKRATVFGQHIHIGCPTGDDAIYLTHALARYVPH
                     FIAISASSPFYLGINTNYCSSRSTIFNAFPLSGVIPYLRSWQEFSDYYRKMYRWKIIE
                     NMKDFYWDIRPKPELGTIEIRVCDTPLTLRKSILITAYIQALALYLLEERPVQLSHDL
                     YYVYNYNRFQASRHGLEGELTVTDKDRPIPIMDDILETIKKIEQYINGLGNGEYIEEL
                     CSDVINKQNDSVLINKIYKQDGSFSKLVAAQCELWLSDSKDRKWMTQPS"
     gene            1411..2442
                     /gene="legN"
                     /locus_tag="ACF2SP_002844"
     CDS             1411..2442
                     /gene="legN"
                     /locus_tag="ACF2SP_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegN"
                     /protein_id="HFD2360610.1"
                     /translation="MDDTTFLSTKEKIQEIKPLMESAFGNILGYQELENMLAICTLVP
                     FSNNQILFHQGDRIKGIYIIIKGTVDLIARTLGKGITKIETCLAGDFLGETSFLLNEP
                     SATSAVAKDNVTCLFIPSAYFELMPAFSPEAKYKIMKAIGYQACGRLKQLHDKIIDFI
                     SHSDMQSLSFLGRVIQTFTQPKTHTLDESGVDKDQIYKKTLFHSFTQDETDELLEHAI
                     PLTANKNCVLIHENETIPVCYIVIYGAVQSNVIQNSKIAKLSVIGPDSLFASTACFLS
                     DTKFSITFITCESATLLKISETELQYFQKNKPMIWYKLFNLICGSIISLARSLKKLDV
                     RLNIEKYNQ"
     gene            2449..3366
                     /locus_tag="ACF2SP_002845"
     CDS             2449..3366
                     /locus_tag="ACF2SP_002845"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFD2360611.1"
                     /translation="MCRILSYLGQPTVVEELLYKPDNSFIKQSYHPKYLPYLLNLAGF
                     GMAAWDRNSINPLQAYVYKTSKLPFYDENLHNLAEKIAPHCLLAHLRGVSYHDKQVVT
                     DQNVHPFLYPVTSIALAHNGMLHNFETMRYDLLKYVKTEYRKYIKGTTDSEWIYAVFL
                     SQLENPKGHFDASEIINAVIATLKILKTIRQQNNIVINSPVNLFLTNGDFIAATRFCL
                     DYGWKPKDAPQSTHFTYHSLWYTYGERYGFYENEYKMKGGTINSSIIIASEPLTENTT
                     TWLEVPEYTLIFANQIDGEIKITSQDINI"
     gene            complement(3494..4549)
                     /locus_tag="ACF2SP_002846"
     CDS             complement(3494..4549)
                     /locus_tag="ACF2SP_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HFD2360612.1"
                     /translation="MVNSDLILNPYQLNEKITLKNRVIMAPMTRNMANDDFSPTRQMA
                     DYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTEAVHERGGKIFLQ
                     IWHVGRVSHPIFLHGNLPISASETMMTGKVRRADELYYGKSRAATLEEINELIQNYAL
                     AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACIN
                     EIGGQSVGLRLSPGAYLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKE
                     LGFKTMTEFMRAHFKGTIIASGSYTLHQARKDIQNKKYDLIALGRPFIANPDLMNRIK
                     WNADFVPYENRMLDTLF"
BASE COUNT         1566 a          956 c          776 g         1462 t
ORIGIN      
        1 tccctagtcc atgaaaaaaa ctccccttca aaataagggc atactcaaag tcgacaataa
       61 caaatattca cttttcatgt aataacatgg aagtattaaa ttcatgcagt gagctcccac
      121 tttaattgcc ctggaatagc agcaaacaag ctaaattatt gaacaatttt tatgggtttg
      181 gggaaaggtg aaccttcata tctattccca ttaaacaacc atctaatcaa aaaccctatc
      241 tgctatactt aaacaaggaa aataatagtc agagaaagga atgagattat tatcttttaa
      301 aaaatccaaa attgtttcaa ttggaactga attggagttt cagattattg attgcagttc
      361 attgtctctt gtttctcgtt caaaagaatt gatgagagcc ttaaaagaca tgcgctatcg
      421 cgaccaaatt aagccggaaa ttacgcaaag catgattgaa atcaattcat caattcatca
      481 atccgctaaa gaaatgtatg acgaactgtt agagctacaa aaaattttag tagaaactgc
      541 tgcaagcatt gatattgctt tttgcggcgg agggacacac ccatttcaac aatggaccat
      601 gcaaaaaatt tttccatcga aacgattcaa aaagaaattt aaccaatacc gttatctcag
      661 caagcgtgct actgtttttg gacaacatat tcatatcggc tgcccaacag gagacgatgc
      721 tatttattta actcatgcct tggcaagata tgtccctcac tttattgcaa tcagcgcttc
      781 atcgcctttc tatctgggta ttaatacaaa ttattgttca tcacgctcga ctatttttaa
      841 tgcttttcca ttaagtggtg tcattcccta tctgagaagt tggcaagaat tctctgatta
      901 ttatcgcaaa atgtatcgtt ggaaaatcat tgaaaatatg aaagattttt attgggacat
      961 tagaccaaaa ccagaacttg gtacaataga aataagagtc tgcgacaccc ctttaactct
     1021 tagaaaatcg attcttatta ctgcttatat tcaagcattg gcactctatc tcctcgaaga
     1081 aagacctgtc cagctgtctc atgatttata ctatgtctat aattacaaca gattccaagc
     1141 cagcagacat ggtctggaag gagaattaac tgtcactgat aaagacaggc ccatacctat
     1201 catggatgac attcttgaaa caataaaaaa aattgaacaa tacattaatg gacttggaaa
     1261 cggcgaatat attgaggaac tgtgtagtga cgtcatcaat aaacaaaatg atagcgttct
     1321 cataaataaa atttataaac aagatggctc atttagcaag ctggtagcag cacagtgcga
     1381 actatggtta agtgattcaa aggataggaa atggatgaca caaccttctt aagtacgaaa
     1441 gaaaaaatcc aggaaatcaa accattaatg gaaagtgctt tcggcaatat tctaggatat
     1501 caagaactgg agaatatgtt ggcaatctgt acacttgtac cattctccaa caatcaaatc
     1561 ttgtttcatc aaggggatag aataaaaggt atttacatca ttataaaagg aactgttgat
     1621 cttattgcca gaaccttagg caaaggaata accaagatag aaacttgcct tgccggtgat
     1681 tttttgggag aaacttcctt tctcctcaat gagcccagtg ccacatcagc ggttgctaaa
     1741 gacaatgtga catgcttatt tatcccttct gcctattttg aactgatgcc ggcattttca
     1801 cctgaggcca aatacaaaat catgaaagca attggttatc aggcctgtgg tcgtttaaaa
     1861 caattacacg acaaaatcat tgattttatt tctcattctg atatgcaaag cttatctttt
     1921 ttggggcgag tcattcaaac tttcacacaa cctaaaacac ataccctgga tgagagtgga
     1981 gttgataaag accaaattta taaaaagact ttatttcatt cttttactca agatgaaacc
     2041 gatgaattgc ttgaacacgc tataccacta actgccaata aaaattgcgt attgattcac
     2101 gagaatgaaa ccataccggt atgttatata gtgatctatg gggcagttca gtccaatgtc
     2161 atacaaaaca gtaaaattgc caaattgtct gttattggtc ctgactcgtt gtttgccagc
     2221 acggcctgtt ttctgagtga cactaaattt tcaattacat ttattacctg cgaatcagca
     2281 actttattaa aaatatctga aaccgaactt caatatttcc aaaaaaataa acccatgatc
     2341 tggtacaaat tatttaattt gatatgtgga tctatcattt ccctagctcg atccttaaaa
     2401 aaattggacg tgcgactgaa catagaaaag tataatcagt gaggcgctat gtgcagaata
     2461 ttgtcttacc ttgggcaacc cacagtggtt gaagagttac tttataaacc cgataactcc
     2521 tttattaaac agagttatca tccaaaatat ttgccttatt tattaaatct ggcgggtttt
     2581 ggtatggctg cttgggatag aaattcaatt aaccccttac aagcttatgt ctataaaaca
     2641 tccaaacttc ctttttacga tgaaaattta cataatttag cagaaaaaat agcacctcac
     2701 tgtcttctcg cccatctacg aggggtttcc tatcatgata aacaagtagt tactgatcaa
     2761 aacgtgcatc catttctcta tccagtaacc agtattgctt tagcacataa tggtatgctc
     2821 cataactttg aaaccatgcg ctatgatctg ctgaaatatg ttaaaacgga atacagaaaa
     2881 tacattaaag gaactacaga tagtgagtgg atctatgctg tttttctttc tcaattagaa
     2941 aatcctaagg gtcatttcga tgcctctgaa atcataaatg ctgtgattgc cactttaaaa
     3001 attttaaaga cgatacgcca acaaaacaac atagtcatta actcaccagt taatcttttt
     3061 ttaaccaatg gtgactttat tgccgcgact cgattttgtt tggattacgg ctggaagccc
     3121 aaagacgctc ctcaatcgac acatttcacc taccattcac tatggtacac ttatggagaa
     3181 cgatatggat tttatgaaaa tgaatataaa atgaaggggg gaaccattaa ttcaagcatt
     3241 attattgcgt ctgagccatt aactgaaaac acaacaacct ggcttgaggt ccccgaatat
     3301 actttgattt ttgcgaatca aattgacggc gaaatcaaaa ttacttccca agatattaac
     3361 atttgaaatg ctctatattt agcatcccaa gacactttct catcatctcc ttgtgccttc
     3421 ttcatcactt cattccaaaa ttttctggta atacagaaag caagaaaggt aattgaatac
     3481 cccgctcaag tgcctaaaac aaggtgtcca acattctgtt ttcataaggt acaaaatcgg
     3541 cattccactt gatacgattc attaaatcag gattagcaat aaatggcctt cccaaagcaa
     3601 ttaaatcgta ttttttattt tgaatatctt ttcttgcctg gtgaagcgtg tagctaccgc
     3661 tggcaattat ggttccttta aaatgagctc tcataaattc tgtcatcgtt ttgaacccta
     3721 attctttgaa ttgaataccg tcatcaaaat tacctgtatg cacataagct attccatagt
     3781 gattcaattc ttccaataca tatcgaaata ccatggcatc tcgctcatct ccgacaattt
     3841 cattaagata agctccggga gaaagacgca atcctacaga ttgcccacct atttcattga
     3901 tacaggcttt taccacttcc agcgcaaatc gccccatatt ctctggatac tgtccatact
     3961 gatcatgacg ttgattggta tggtaatgta aaaactgatc aatcaaataa ccattggcac
     4021 catgaatctc aatgccatca aacccggcta tcattgctct tcttgcagcc aacgcatagt
     4081 tttggattaa ctcattaatt tcctctaatg tagccgccct tgattttcca taataaagtt
     4141 catcggcacg ccggacctta cctgtcatca tcgtttctga agcagaaatt ggtaagttac
     4201 catgtaaaaa aatcggatga gataccctgc caacatgcca aatttgcaaa aatattttcc
     4261 ctccccgttc atgcacagct tccgttacca ttttccaacc gtcaatatgg gtttgcgtaa
     4321 atattcctgg agcattacta taaccttgac catcagggct aataatagtc ccttctgtaa
     4381 taatcaaccc ggcatcagcc cttcgagcat aataatctgc catttgcctg gttggactga
     4441 aatcatcgtt agccatatta cgagtcatgg gagccataat gactctgttc tttagtgtaa
     4501 ttttctcatt aagctgataa ggatttaaaa ttaaatcgga gttaaccatg ataaatctcc
     4561 tgcatgtcat aataatatat tggaatatcg caaatattcg atttattaaa gcaaaatttt
     4621 ttaaatcata actttcaatt cttttataaa ttcattaatt aggtttgcat tcgctttaca
     4681 ataggtccac tgtcctctac gctcagtgaa tatcaaacca gcttgctgta attgtgataa
     4741 atattgagat accgtggatt
//
LOCUS       DAXLDT010000210         4757 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXLDT010000210 DAXLDT010000000
VERSION     DAXLDT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.211"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..664
                     /locus_tag="ACF2SP_002847"
     CDS             <1..664
                     /locus_tag="ACF2SP_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LbtU family siderophore porin"
                     /protein_id="HFD2360613.1"
                     /translation="LGQTNEVTAIIGYDSLYYANASFFKPYSRINTSSLPLYYNLNTG
                     VHNQSMDAGVSYLYSLAESQLFQYNKGFGGLLSQSLKTRVPGFATYFNLKYKKTSTYL
                     TYVTAITPFALEDMSYNNRRASPKALSIQNSYDANIKKFSFKIIGFYDYTFQALALGI
                     PKQRLGLGLSATPFPYLGIQFQYSRDYSYGNNAIASGINHFVNGRNTKTNNLALQTIL
                     NF"
     gene            797..2539
                     /gene="lbtA"
                     /locus_tag="ACF2SP_002848"
     CDS             797..2539
                     /gene="lbtA"
                     /locus_tag="ACF2SP_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin biosynthesis protein
                     LbtA"
                     /protein_id="HFD2360614.1"
                     /translation="MAAVLNNSYKINNSLHSLLLEKDLLVSQSEMEHFISIAHQQCLD
                     RLQNAALREGLIDKEINSLHIHDYLDLLYHQQKKQKPEKTFDRWPLLKKELDESINNQ
                     ALALAYYHCWQQSLKQQAKNHSSLWSWLSEQHNKQQILTFLEQWGCIGHPYHPNFRAK
                     MGFIPEEVIRYSPEFNAQVDIPWLAIHRSVAFTSIDATSYSLLFAQHFAKEYQLWNYS
                     VQKQQLDPEQYLPLPVHPWQWNNKLQTLASALINSKQLLITEAFQKTKPSMSFRTMMP
                     IDNKGPHLKLATAIHTTSALRTVSPASVSNSSPLSSWLRQLLTQYQSFEKPLFLARDL
                     AGINTTHPSISPQNKKHLAMIVRENPLELIDASQTLVPVAALYALSPVSQRALFIEII
                     EASGMDPELYFIEYSRCILECQLHLLLSYGVALEAQQQNTLVIFQSHRPVGLVIRDLG
                     GIRVCFNSLYETTPKPNLHPDSTISCHELSDLFNTFIYGNLLSNLTFCINCLHDNYNL
                     TKKNLWILLKQIANQLLQNFKKEINPLIYQKFQQHLFSLPWQHKSLLSMRLNQNQDNL
                     VFFSLDNPLSQDNE"
     gene            2532..3746
                     /gene="lbtB"
                     /locus_tag="ACF2SP_002849"
     CDS             2532..3746
                     /gene="lbtB"
                     /locus_tag="ACF2SP_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin export MFS transporter LbtB"
                     /protein_id="HFD2360615.1"
                     /translation="MNKPVQKMMLSGQFLMILALEMTNPFLPLLIAHQSNTPVPSVVI
                     YNTLSLILPMLANILLTPIWGLAADRYGYKPMLMRASWALVLTQASMIFVNSVFWILI
                     IRLLQGAFAGFLVAMQTYALSITEWQNKSTQLSRLQSSKAIATSTAGFLGGLALSFTN
                     YQGLFGLATLICMGTTVAMHYKLPSPPKHQIKKKSQTLNHSYTSKSVFFFLCVLIMLT
                     QIAKFLPDPGFTLYLNKYCSNNLVLIGFLYSLPAMGMLCSSVWCGKQFDYCRSQPSLV
                     NQYLIRYSVFGAILMVIQANVDNFYMFGLIRILWGVVLAALLPALFALCSDRNLLPGY
                     ALGLANSFAKLGNLIGLLLGGLFAFYLPYPAIFLIIAGIYSLFAIFVYGYERLNVRQM
                     TDFSNSYFNVKS"
     gene            3743..>4757
                     /gene="lbtC"
                     /locus_tag="ACF2SP_002850"
     CDS             3743..>4757
                     /gene="lbtC"
                     /locus_tag="ACF2SP_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038838172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin import MFS transporter LbtC"
                     /protein_id="HFD2360616.1"
                     /translation="MKKNALLIVLLTWFLGNMDIHFLTPALPSLVDYFSVTPNTAQLT
                     ISLFLLGKALSMILWGLLSERYGRKPIFIGGLLLFILSNFLIALNHSILLFLVCRFLQ
                     GISVGATLLMGRAMINDTHNEQNATKYFAWLFTLAGLFICFLPFFGGLINSYWNWQIA
                     SLIIAAYGLLLLPLCRGLKETKPHYVKFPRLGHSIMVVFNHPLFVSYLLISALMMAGE
                     SAFNTSASFILIKGENFTSAQYGTIKTTMAIMHLLGTACCGLIAKYYHSIQLTKLGVR
                     FFIFAAFLMWLFVFSAENIYLTFIVPMVIYYFGTGFIVASATASAVRPFPQQMALALA
                     ITLF"
BASE COUNT         1432 a          961 c          788 g         1576 t
ORIGIN      
        1 cttgggacaa accaacgaag tgaccgctat tattggatac gattccttgt attatgctaa
       61 cgcttctttt tttaaaccct attctcgcat aaatacatca tcacttccgc tttattacaa
      121 tctgaataca ggagtacata atcaatcaat ggatgcggga gtcagttatt tatactctct
      181 ggcagaaagt caattatttc aatacaacaa aggttttggc gggcttttat cacaatcatt
      241 aaaaacaagg gttccaggat ttgcaaccta ttttaattta aaatataaaa aaacaagcac
      301 ttatcttacc tatgttactg caatcacccc ctttgcatta gaagacatga gttataacaa
      361 tcgaagagct tcgccaaagg cattgagtat acaaaatagt tatgatgcta atattaaaaa
      421 attttctttt aaaatcattg gtttttatga ctacactttt caagctctgg cactcggaat
      481 accgaaacaa agactgggac tgggattgtc cgcaacccct tttccttatc ttggtattca
      541 atttcagtat tccagagact atagttatgg aaataatgcc atagcctcgg gtatcaatca
      601 ttttgtaaat ggccgtaata caaaaacgaa taatttggca ttacaaacca tactgaattt
      661 ttaaattcag gtactcaatc aaaaataaaa aactatctct ctattattta agcaaataaa
      721 tcacaataca ttattgcaaa tgataatcat tatcatttat agtgttgtcg tttcttactt
      781 attcacggaa ataatcatgg cagcagtttt aaataattct tataaaataa ataactcatt
      841 acacagttta ttacttgaaa aagatcttct tgtttcacaa tcagaaatgg aacatttcat
      901 atccatcgcg caccagcaat gtttagatcg attgcaaaat gcagcacttc gtgaaggatt
      961 aatagacaag gaaataaaca gccttcatat acatgattat cttgatttac tttatcatca
     1021 acagaaaaag caaaaaccgg aaaaaacatt tgatcgatgg cctcttctaa aaaaagaact
     1081 cgatgaaagc atcaacaatc aggcgttagc cctcgcttat tatcattgct ggcaacaatc
     1141 gctgaagcaa caggcgaaaa accactccag tttatggtca tggttatcag agcaacataa
     1201 taaacaacaa attctgactt ttttagagca atggggttgc atagggcacc cgtatcatcc
     1261 taatttccgc gcaaaaatgg ggtttattcc tgaagaagtg attcgatact cccctgaatt
     1321 taacgcgcaa gtagatattc cctggctggc aattcatcgc tccgtagcgt ttacttcaat
     1381 tgatgcgact agctattctt tgttgtttgc ccaacatttt gctaaagaat atcaattgtg
     1441 gaattattct gtccaaaaac aacaattaga tcccgaacag tatttgcctc ttccagtcca
     1501 tccctggcaa tggaataaca aattacaaac tttggcctct gcattaatca attcaaagca
     1561 attattaata acagaggctt ttcaaaaaac caaaccatcc atgtcgttcc gaacgatgat
     1621 gcctatagat aacaaaggtc cacacttaaa attagctaca gcaatccata ctacttctgc
     1681 tttacgcact gtatctcctg catcagtaag caattcgtct cctctttcca gttggcttag
     1741 gcaattactg actcagtatc aaagttttga aaagccacta tttctagcca gggatttagc
     1801 ggggattaac accacacacc catctatttc cccacaaaat aaaaagcatt tggctatgat
     1861 agtacgtgaa aatccactgg aattgatcga tgcgtcacaa acattagtac ctgtagctgc
     1921 actctatgct ctttctccag tcagtcaacg tgctttattc attgaaatta ttgaagcaag
     1981 tggcatggat cctgaattat attttattga atacagccga tgtattctcg aatgccagct
     2041 tcatttatta ttaagctatg gtgttgcgtt ggaagcccaa caacaaaaca ctttagtcat
     2101 ttttcagtct catcggcctg ttggattagt tattcgagat cttggcggca tcagggtctg
     2161 ttttaattcg ctatatgaga caacacccaa acctaatctt catccagact ctaccatttc
     2221 atgccacgaa ttgagtgatc tctttaatac cttcatttat ggaaatttac tgagtaactt
     2281 aactttttgc attaattgtt tacatgataa ctataacttg actaaaaaaa acctttggat
     2341 cctgctaaaa caaattgcaa atcaattatt acaaaatttc aaaaaggaaa ttaatccatt
     2401 aatataccaa aaattccaac aacatttatt ctctcttccc tggcaacata aaagtttgct
     2461 gagcatgaga ctcaatcaaa atcaggataa cctggtattt tttagtcttg acaacccatt
     2521 gagccaagat aatgaataaa ccagttcaaa aaatgatgct ctcaggtcag tttttgatga
     2581 tattggcctt ggaaatgacg aatccttttc taccactatt gatcgcgcac caaagcaaca
     2641 caccagtacc atcggtagtg atttataaca cgctgagtct aattctgccg atgctggcta
     2701 acattctatt aaccccaatc tggggcttgg cagctgatcg atatggctat aaacccatgt
     2761 taatgagagc atcctgggcc ttggtgttga cccaagccag tatgattttt gtaaattctg
     2821 tattctggat tttaatcatc cgattactgc aaggagcttt tgcaggtttt cttgttgcaa
     2881 tgcaaaccta tgccctaagt attactgaat ggcaaaacaa aagcacacaa ttatctcgct
     2941 tgcaatcctc caaggcaatt gccacatcta cagccgggtt tctcggagga ttagctttaa
     3001 gcttcactaa ctatcaagga ttatttggtt tggcaacatt gatatgcatg ggcactactg
     3061 tagcgatgca ctacaaatta ccctcgccac caaagcacca gataaagaaa aaatcacaaa
     3121 cattaaatca ttcttacaca tcaaaaagtg ttttcttctt tctttgcgta ttaatcatgc
     3181 taacccaaat tgccaagttt ttacctgatc cagggtttac cctgtatcta aataagtatt
     3241 gttcaaataa tctggtacta attggatttc tttattcttt accagcaatg ggaatgctat
     3301 gtagttcagt atggtgtggc aaacagtttg actactgtcg aagtcaacct tctttggtta
     3361 accaatattt aatacgctat agtgtattcg gagccatcct catggtcatt caagccaatg
     3421 tagataattt ctatatgttt ggccttatac gcattttgtg gggggtcgta cttgcggctt
     3481 tattgcccgc attatttgct ttgtgcagtg atcgtaattt gttaccaggt tatgccttgg
     3541 gcttagccaa ttcatttgca aaactgggaa atctaattgg attgttattg ggtggtcttt
     3601 ttgcatttta tctaccgtat cctgcaattt ttctgataat tgccggaatt tatagtttat
     3661 tcgcaatttt cgtttacggg tatgaacggc taaatgtcag gcagatgaca gatttttcta
     3721 attcttattt taacgtgaaa tcatgaaaaa aaatgctctt cttatagtgt tattaacctg
     3781 gtttcttgga aacatggata tacatttcct tactccagcc ttgccatcat tagttgatta
     3841 cttctcggta actcccaata cagcccaact aactatcagt ctttttcttt tggggaaagc
     3901 cctcagtatg attttatggg gtcttctttc cgaacgttat gggagaaaac caatcttcat
     3961 tggaggactg ttgttattta ttctgagcaa ttttctgatt gctctaaacc actccatcct
     4021 tctcttttta gtatgccgat ttctccaggg aattagcgta ggcgctaccc ttctaatggg
     4081 tagagcaatg attaatgata cccataatga acaaaatgcc acaaaatact ttgcgtggtt
     4141 atttactctg gctgggctgt ttatttgctt tcttccattt tttggaggtt tgataaacag
     4201 ttattggaat tggcaaatcg cctctcttat cattgctgcc tatggcctgc ttttattacc
     4261 tttatgtcga ggtttaaagg aaaccaaacc ccattatgta aaattccctc ggctaggtca
     4321 tagtattatg gtggtcttca accatcccct ttttgtttct tacctcctta tttctgcctt
     4381 gatgatggct ggagaatcag cgtttaatac cagcgcctct tttattctta ttaaaggaga
     4441 aaattttact agcgctcagt atggtacaat caagaccact atggcaatta tgcatttatt
     4501 aggtaccgct tgttgtggct taatagcaaa atattatcat agtatccaat taacaaaatt
     4561 aggagtacgt ttttttattt tcgcagcatt cttgatgtgg ttatttgtct tttctgctga
     4621 aaatatatat ttgactttta tagtgcctat ggtgatttat tattttggta caggtttcat
     4681 tgttgcatca gcaacagcat cagctgttcg cccttttcct cagcaaatgg cccttgcatt
     4741 agctatcact ttatttt
//
LOCUS       DAXLDT010000211         4745 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXLDT010000211 DAXLDT010000000
VERSION     DAXLDT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.212"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            233..580
                     /locus_tag="ACF2SP_002851"
     CDS             233..580
                     /locus_tag="ACF2SP_002851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360617.1"
                     /translation="MEPLDKDITYFVVGNPIATSEVSLFSVIPEVRKKISDEEIKGAI
                     PTGDALLFRTREEAEKYARITANFGSKGEIRHKAPVFEVQLRNPVAEPQTVTKTLRKD
                     RYTLDHIEIQWQR"
     gene            568..1158
                     /locus_tag="ACF2SP_002852"
     CDS             568..1158
                     /locus_tag="ACF2SP_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360618.1"
                     /translation="MAKVDADNLRYISGGVPGIPGKYQFKPTDEANVKTPKEWLDKIG
                     FESKIEALQKKVGGLYKKDVNAAEELEDILKELRANKKEFVEGRKNAKQFVDACTDAI
                     KPEKTKALAGHRGLFASIKSFVESIVQLVNPKFKINSDSINKIKDVEKSLGEIKPEQV
                     VHETDIAKRTLKSDYEVDSENKVAKTGQGGPSTKPE"
     gene            1424..2095
                     /locus_tag="ACF2SP_002853"
     CDS             1424..2095
                     /locus_tag="ACF2SP_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFD2360619.1"
                     /translation="MWVPEISLLDHLPGYIGWKDLNRHYIGANKALLELKGFRHVEEL
                     AGKTDEELSPWAIEENKLFQQQDFRVINGEKVATVHFNAKTSEVFLLEKCPLTDQNNQ
                     VTGLIYHCRPCTKNEVFRILKQLDDKWHISPPHYTPNDNKNIYGLTEREHECVFLLIR
                     GKSAKGIGALLSLSKRTVESYIENIKNKMDCRNKAEILVKAVSNGYHNHIPARLNQAA
                     VIKSL"
     gene            2200..3183
                     /locus_tag="ACF2SP_002854"
     CDS             2200..3183
                     /locus_tag="ACF2SP_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HFD2360620.1"
                     /translation="MRQLFIIMFIFLLSNAGYCAPAFEILPLGVYGGLKDGNLSAYLI
                     KGIESKNYTALDGGTLVHGLEVAVDKQSVRNKNVDNLLQKHIPAYLISHAHLDHVMGL
                     VMAQPELREKQTIMAREETMQALQKNIFNWSVWGNFGDSGEIPHLNYQHYQTTPLLEW
                     VAIPDTDLHVKIFPLSHGGMASSAFLIRYKTEYALYFGDTGADRIEKSTNIENIWKEI
                     APLIRNKQLHAIMLECSFLNSQPDDKLFGHLKPNLFMSELRHLASIVDPFDSRNSLRG
                     LPVVVTHIKPRLADFSNTNEDTEKQIINELSQENDIGVELIKPEQGITLSL"
     gene            complement(3365..4369)
                     /locus_tag="ACF2SP_002855"
     CDS             complement(3365..4369)
                     /locus_tag="ACF2SP_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2360621.1"
                     /translation="MKQTAIVLQGGGALGAYELGVLKYLYASDSFSPNIISGVSIGAI
                     NAATLIGAKDDPIQTLEAMWEELTVFSAQIFPENAESYLSLFGNAAFYHLRTDYWSMP
                     YWTNFYTTIPLQQLLLKYIDFDKLNNSPIHLILTATNVTTGKSAIFENQGTNRTEITP
                     LHVLASGSLPPGFPMTLIGDTYYWDGGLFSNTPLSPVMEKLNPDPNVEKQIIVINLFP
                     SRGKIPTNMPEVFDRVFEIIFSNKIRFNTELTEKINEYIETIQEIEKVIPENSFIRQL
                     PGYKRLLNYKYIQDIIYIESSDEENATASFDFSRKAIQRRINAGYKDAKNNFQLNQLI
                     "
BASE COUNT         1547 a          819 c          986 g         1393 t
ORIGIN      
        1 gatatgtcga atttgcgaca taaagtggtt ttctcgttaa aagacaagag tgcgttaata
       61 tacgctgtaa gttatatgtc gtaacggtta tgaattttgg atttcaagaa aacagcctgg
      121 taactttcgt ctttctacgc tctgtatttt gccttgtttt ttggcaatca attatagatt
      181 tctttttttt gagctaaaat taaactaggt taagctcatg ggagtttatt atatggagcc
      241 gttagataaa gacattacgt attttgtggt aggcaaccca attgcaactt cggaagtttc
      301 tttattcagc gtaatccccg aagtaagaaa aaaaatttcc gatgaagaaa taaaaggtgc
      361 tataccgact ggagacgcat tgttgtttcg tacgcgagag gaagctgaaa aatatgcgag
      421 aattacggca aattttggtt ccaaaggaga aattagacat aaagctcctg tatttgaggt
      481 tcagcttaga aatcctgttg cagaacctca aacggttaca aaaacattac gtaaagatcg
      541 ttatacttta gatcatatag aaatacagtg gcaaaggtag atgctgataa tttgcgttat
      601 atttcaggag gagttcctgg gattccgggt aaatatcaat ttaagccaac agatgaggca
      661 aacgttaaaa ccccaaagga atggttagat aaaatcgggt ttgagagcaa gattgaagct
      721 ctgcagaaaa aagtgggggg actctataaa aaggatgtca atgcagcaga agaactggaa
      781 gacattctta aagaattaag agccaataaa aaggaatttg ttgaggggcg caaaaatgct
      841 aagcaatttg tagatgcctg cacagatgcg ataaagcctg aaaaaacgaa agcactggca
      901 ggacaccgtg gactttttgc ctcaattaaa tcatttgttg aatctatcgt tcaactggta
      961 aacccaaaat ttaaaattaa ttcagattct attaataaga taaaagacgt tgagaaatcg
     1021 ttaggtgaaa tcaagccaga gcaagttgtg catgaaacag atatcgcaaa aagaaccctt
     1081 aagtctgact acgaagtgga tagtgaaaat aaagtggcta aaaccgggca gggtggccca
     1141 agtactaaac cggagtagtt caacagggta gtttcatatg aattgcgcat ccataatctg
     1201 acatgccact cgctattaaa aaagcctatg gttgataacc aatactttaa ttgcatacca
     1261 gcgcttcaga aatggcaata aaatgattga ctattaagac agccatagct caggctcggc
     1321 tctgatttat aagcagctga ttctgccaat ctgccttgaa aattacgccc caaccggtta
     1381 ataatccacg ctcaaaatga gcattcattg gaggatgggg ctaatgtggg ttcctgaaat
     1441 atcattgctt gatcatctcc caggctatat tgggtggaaa gatttaaacc gccattatat
     1501 aggcgcaaac aaagcgttgt tggaattgaa agggtttcgt catgtagaag agcttgcggg
     1561 taaaacagat gaagagctgt cgccgtgggc tattgaagag aataaactgt tccagcagca
     1621 agattttcgc gtaataaacg gtgaaaaagt agcaacagtt cattttaatg caaagactag
     1681 cgaagtgttt ctgttggaaa aatgtccttt aacagaccag aataatcagg taacaggatt
     1741 gatttatcat tgccgtcctt gtaccaagaa tgaagttttc aggatattaa aacagcttga
     1801 tgacaaatgg catataagcc cccctcatta cacgccgaac gacaataaaa atatctatgg
     1861 attaacagag cgtgagcatg aatgtgtgtt tttattaata aggggaaaat ccgcaaaagg
     1921 aataggggct ttgctttctc tatccaaacg gactgtagag tcgtacatag aaaatatcaa
     1981 gaataaaatg gattgcagga ataaagctga aatactggta aaagcagtgt caaatggtta
     2041 tcacaatcac atccctgcaa gactaaatca agcggctgtt ataaagtcac tttaatcatt
     2101 atgatgttga acttctaccg tataaatacg gtgattaaag caaggttgaa tacggtatta
     2161 ttttacaaac tgacctaaat tatttgaaag gaaatcccca tgcgccagct attcattatc
     2221 atgtttattt ttttattatc taatgcagga tattgtgcgc ctgcttttga aattcttccc
     2281 cttggagttt atggggggtt gaaagatgga aatttatcag cttatttaat caagggtatc
     2341 gaaagtaaaa attatacggc tcttgatggt ggtactttgg tgcacgggct tgaagttgca
     2401 gtcgataaac agtcggtgcg taataaaaat gttgacaatt tgctgcaaaa acatattcca
     2461 gcctacctta tttctcatgc ccatctcgat catgttatgg ggcttgtgat ggcgcaaccc
     2521 gaattgcgag aaaagcaaac cattatggca cgtgaagaaa ccatgcaggc attgcagaaa
     2581 aatattttta attggtctgt ttgggggaat tttggagata gcggagaaat ccctcatttg
     2641 aattatcaac attaccaaac taccccatta ctagagtggg ttgctattcc tgatacagat
     2701 ttgcatgtta aaattttccc tttgagccat ggtggaatgg catcaagcgc tttcttaata
     2761 cgttataaaa ccgaatatgc cctctatttt ggtgatactg gtgctgatcg cattgaaaaa
     2821 tcgaccaata tagagaacat ttggaaagaa attgcgccac tcataagaaa taagcagctg
     2881 catgccatca tgttagagtg ctcattttta aattcacagc cagatgacaa attatttggc
     2941 catttaaaac caaacttgtt tatgtcagag ttgcggcact tggcatcgat tgttgatcct
     3001 ttcgactcaa gaaactcact acgaggttta cccgttgttg ttacacacat caaacccaga
     3061 ttagctgact tttcaaatac taatgaagat acagaaaaac agattataaa cgaattgtcc
     3121 caggaaaatg atattggcgt agaattaatt aaacctgagc aaggtataac cttaagttta
     3181 tgattgatga agagttacaa cagccgccag aaccagccct aataaatcat ttatggtatt
     3241 tgtgtatcag gggctgttgc gaaaaatccg aacaagaata aaaaaacctt agcatagaga
     3301 agttgctact cctgcaactt tgcatgctat gacagcagca atgtaatagt ggatgcagca
     3361 gatttcaaat taactgattc aactgaaaat tatttttggc atctttgtat cctgcgttaa
     3421 tacgtctttg tatcgctttg cgggaaaaat caaaagaagc ggttgcgttt tcttcatctg
     3481 agctttctat ataaatgata tcctgaatat atttgtaatt aagcagtcgt ttatagccag
     3541 gaagctgtct tataaagcta ttttccggaa tcactttctc aatttcctgt attgtttcaa
     3601 tgtactcatt aattttttcg gttaattcgg tattaaaacg gattttattg gaaaaaatga
     3661 tttcaaacac tctgtcaaaa acttcaggca tattagtcgg tatttttccc cgactgggga
     3721 aaagattaat cacaataatt tgcttttcaa cgtttggatc ggggttaagt ttttccataa
     3781 ccggagatag cggagtattg gaaaataaac ccccatccca gtaataagta tctccaatga
     3841 gtgtcatggg aaagccagga ggcaaactac cgctggccaa tacatgtaaa ggcgtgattt
     3901 ctgtacggtt agtaccctga ttctcaaaaa tggcactttt acctgttgta acattagttg
     3961 cagtaagaat tagatggatt ggactgttat tcagcttgtc aaaatcaatg tattttaata
     4021 ataattgctg aagaggtatt gtggtataaa aattggtcca ataaggcata gaccagtaat
     4081 cggtacgcaa atggtaaaaa gcagcattgc caaagagtga cagataagat tcggcatttt
     4141 ctgggaatat ctgggcagaa aaaacagtca attcctccca cattgcttcc agagtctgaa
     4201 taggatcgtc ttttgcccca atcagtgtcg ctgcgttgat ggccccaata gaaacgcctg
     4261 aaataatgtt tggcgaaaaa ctgtctgatg catagaggta ttttaatacg cctaattcat
     4321 aagctcccag tgcccctcct ccctggagta caatggccgt ttgcttcatg gatgtcctta
     4381 tcatttcact ctgcatgaag tatagcaggt aattataatg aaaaggatgt gatgcgagtc
     4441 ctggattgtc agaagcaagg tatattactg tccagttaaa ctaggtctga ctttgatagg
     4501 gtagggcgca aaagatggct ccaaaccggt tttaagcatt tgttaagtat tttgactttc
     4561 ctggaaaaaa tattattatg aataattcta ttagggatta aatgacctgc ttttttagag
     4621 cagcgataac tccagacata caaagccttg cttacacgat gtgtattgtt gtaatgaacc
     4681 ggggtaatca gataaatgct ctctaaagct tgatgatacc tgcatctgaa taagattatt
     4741 cttat
//
LOCUS       DAXLDT010000212         4656 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXLDT010000212 DAXLDT010000000
VERSION     DAXLDT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.213"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            102..178
                     /locus_tag="ACF2SP_002856"
     tRNA            102..178
                     /locus_tag="ACF2SP_002856"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136..138,aa:Met,seq:cat)
     gene            392..1528
                     /locus_tag="ACF2SP_002857"
     CDS             392..1528
                     /locus_tag="ACF2SP_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFD2360622.1"
                     /translation="MFRINLIRYVALLFIITLNLDCISVAFSSQVIYGTQNAARVTGK
                     SLKKIYFLQLGAFTQKNNAQQLARKIQARTQYPVIISHSGNAFSVRVGPVNTQELHQL
                     SIKLSQNSNITPIKHNSSPKQTPTIQTKSQILVKENSRPLPTHSVTKETIDGKGTWYV
                     TAKIGAQEITPDDQFTVNNDSGFPSPFDKDIYTNSSDSSLLLGIGGGYRFTNIILPAI
                     SVGVYYSHFFNMDINGQVIQYSLPEFTNYNYQWKVKSDLVLASVKANAFRWKNLMPFV
                     QGGVGASSNRASQYYENALPDVTPRISPNFANHTATVFAYQLGAGLDWEIRPQWLLSL
                     GYEYSDLGKVRSGYGTDSWNRETLSSKNLTSNAGTFGFTYLFHG"
     gene            1581..4058
                     /gene="legT"
                     /locus_tag="ACF2SP_002858"
     CDS             1581..4058
                     /gene="legT"
                     /locus_tag="ACF2SP_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegT"
                     /protein_id="HFD2360623.1"
                     /translation="MWLPNGNLSNRTDLIFIKIMGERTNMKYSTRIIITTILLLFALL
                     GYAGKDPISWRVSGVFPAQSSVGQTYTVTYTFTNQLPFRLAKALIIERAASPNTEFNF
                     NDQCSGRLLNSGESCTVVVALNPLIIGLKTLQLTIAGYSKDRVPLPQTSTLSTGQSLS
                     PIVSFVMDGFPSQLATGSTASYKFTFTNNGPISATNLSVNVSQSLGAPNFTTTCNNTL
                     TPNGGVCEVDGTYTVTSGSPSIQQVIASLSYTGPSGSPVTAQTFTQVVNPAAPLVGTL
                     VPPNYLPPLMIASPPPYTVQFLFTASGTVDITNNGTITCLEGVNNCNGTINITSSSCT
                     PQTLTNAACQMTATFTAPPATIPPAAPLTYILTATVPYSVGGNPQPDATAITSGTVLT
                     ALPTERTVTLINECDFEVWFSLNGSQLGSSPNCPTTPCPNGTSCNTSTNKCFWNNPAP
                     NNGIYSLPALPPPANTNSVTIPVTNADPNIQWSGNISASTLCNGTTCQQAACGNNGGT
                     TSCAPGIGFTQPATQAEITMNLTTSDSYDVEVINGFHIPISMQPIYYQGVTTIPATPD
                     NYNCGEPGKDTAANGFGACDWSTATVPVIDQVPGNGFYWVTGGGQGCSITSANPGCPA
                     MTLCGLDSNFNQVCGNFLGYWSADQVCGSSNVPAAVQSYFKCNQPLPTSTTPFYPSGA
                     VLSNLMLCSVPTGFTGPRYNTCYNAYPSSSPTDIAQCCGCADWWNPAQTNNVAIGANP
                     NTESCTQPGALQPQTNAQWNSFVQPMIQWMKRACPSAYIYPFDDKTSGFTCTNNLSGQ
                     PNSTSYIIRFCPGGITGLPAGVNEGRG"
     gene            complement(4138..4452)
                     /locus_tag="ACF2SP_002859"
     CDS             complement(4138..4452)
                     /locus_tag="ACF2SP_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HFD2360624.1"
                     /translation="MVIYEVNLTIDLEIFVQFQAWLKKHVAEIIQFPGFMQACILKQE
                     KETKSGKEKLTVQYQLDNRDNLSRYLSELAPKMREEGIHLFQAKFSADRRIFEVQDII
                     LK"
BASE COUNT         1417 a         1070 c          841 g         1328 t
ORIGIN      
        1 aaaaaataat gcaaattcaa tttaatataa attaattata aaaaactcag gaaacagtct
       61 tgacataaac acatgaaaaa gacaaaatca cgacctcttg tggctatgta gctcagccgg
      121 ttagagcgcg gcactcataa tgctgaggtc ggtggttcga gtccacccat agccaccatt
      181 tacatcatat gccccctgat tgagaacact tgaagtaagt aatcaaaccc tactcccgat
      241 ttttgtctct catttgtcac ttcaagcaat agaatatcca ctataatcaa atcagaccct
      301 gcccagaaat aatatttgag atcatcttgc atcacgaaaa gaaattatag tagcatcgta
      361 gctcatcaca tttcataata cggattttat tttgttcagg attaacttaa tacgatatgt
      421 ggcacttctg tttattatca cattgaattt agattgtatt agcgttgcat tttcatctca
      481 ggtaatttat ggaacacaaa atgctgctcg agttactgga aaaagtctga aaaaaattta
      541 ttttcttcag ttaggcgctt ttacccaaaa aaacaatgct cagcaattgg caagaaaaat
      601 tcaagcgaga acacaatacc ctgtaataat tagtcattca ggcaatgctt tctcggttcg
      661 tgtggggcct gtcaatacgc aagaactaca tcaactcagc ataaaacttt cacaaaattc
      721 caatattact ccaataaaac ataattcatc accaaagcag acaccgacta ttcagacaaa
      781 atcacaaata cttgttaagg aaaattctcg ccctcttcca actcattctg tcactaaaga
      841 aacaattgat gggaaaggaa cttggtatgt aactgctaaa attggagcac aagaaataac
      901 accagatgat cagtttacag taaataacga cagtggcttt cccagccctt ttgataaaga
      961 tatttatacc aacagttcag attcctccct gctattaggc attggtgggg gatatcgatt
     1021 tacaaatata attctccccg caatctctgt tggagtatac tattcccatt tctttaatat
     1081 ggacattaat gggcaggtga ttcagtacag cttacccgaa ttcaccaatt acaattatca
     1141 atggaaagta aaatctgatt tggttttagc ttctgtaaaa gccaatgcct ttcgctggaa
     1201 aaatttaatg ccatttgtac aaggaggtgt tggagcgagc tcgaatcgag caagtcaata
     1261 ttatgagaat gccttgcctg atgtcacacc tcgtatcagt cctaattttg ccaatcatac
     1321 ggccactgta tttgcttacc aactgggcgc tggccttgat tgggagataa ggccacaatg
     1381 gttgttatct ttagggtatg aatattctga tttaggcaaa gtacgctcag gttatggtac
     1441 tgatagctgg aatagagaga cattgtcctc gaaaaattta acaagcaatg ctggaacctt
     1501 tggctttacc tatttatttc atggataacc aaaagtgagg cacgggataa aattccaaac
     1561 cttgagaaac cacaaataat ttgtggcttc ccaacggtaa cttatcaaat agaactgatt
     1621 taatcttcat aaaaataatg ggtgaaagga ccaacatgaa atactcaact cgcatcataa
     1681 tcaccacaat ccttctgtta ttcgccttgc tgggttatgc tggaaaagat cctataagct
     1741 ggcgagtcag tggagtattt cctgctcaaa gcagcgttgg tcagacttat actgtgactt
     1801 atacttttac caatcaattg ccttttcgat tggccaaggc cttgattata gaacgagcgg
     1861 caagccccaa cacggaattc aattttaatg atcaatgttc cggtcggtta ttaaattccg
     1921 gagaatcctg tacggtagtt gtggcgttaa acccccttat aatcggtctt aaaacccttc
     1981 aattgactat tgcaggatac agcaaggata gagttcctct acctcaaaca agcaccttaa
     2041 gcacaggaca gtccttgagt ccaattgtta gctttgtcat ggatggcttt ccctctcaat
     2101 tagcgacagg ctccactgcc agctataaat ttacctttac caataatggt cctatatctg
     2161 ccaccaattt atcagttaat gtctcgcaat cattaggagc acctaatttt actacgacgt
     2221 gcaataacac attgactccc aacggagggg tttgtgaagt cgatggaacg tacacagtaa
     2281 cttccgggag tccgtcgatt caacaagtca ttgccagttt gtcttataca ggcccatcgg
     2341 gaagcccggt aacggcacag acattcacac aagtagtcaa tccagcagct ccgcttgtag
     2401 gcactttagt accgccaaat tatctaccac cattaatgat tgcttccccc cccccgtaca
     2461 cagtgcagtt tttattcaca gccagtggaa ctgtagatat tacaaataat ggcacaataa
     2521 cctgcctgga aggtgttaat aattgtaacg gaactattaa tatcaccagc tccagctgca
     2581 ccccgcaaac attgaccaat gcggcctgtc aaatgactgc cacgttcaca gcaccccctg
     2641 caacaatccc ccccgctgcc ccacttactt acattctaac tgcaacagtt ccctattccg
     2701 ttggaggaaa cccacaacct gatgcaaccg ccatcacttc cgggacagta cttaccgcat
     2761 taccgacaga aagaacagtg actttaatca atgagtgcga ttttgaagta tggttttcgc
     2821 tgaatggaag tcaattaggt agttcaccca actgcccaac aacaccatgc ccaaatggca
     2881 caagctgtaa caccagtacg aacaaatgct tttggaacaa cccagctcca aacaatggaa
     2941 tatattcatt accagccctt cctcctcccg ctaatactaa cagtgtaact atccctgtta
     3001 ccaatgcaga ccccaatatt caatggagtg gaaatatttc ggcaagtacc ctgtgcaatg
     3061 gtacaacctg ccaacaagca gcttgcggta ataatggcgg cactacctcc tgcgcgccag
     3121 gcattggttt cacacaacct gctacccagg ctgaaattac tatgaattta accacctccg
     3181 acagttatga tgttgaagtc atcaatggtt ttcacatccc gatttcgatg caacccattt
     3241 attatcaagg agtcactact attcccgcta ctcctgataa ttacaactgt ggagaacccg
     3301 gcaaagacac tgcagccaat ggatttggag cttgcgattg gagtacagcc acagtgccag
     3361 tcattgatca agtgccaggg aatggatttt actgggtaac tggtggagga caaggttgtt
     3421 ccattacctc tgcaaatccg ggttgccctg cgatgacatt atgcggatta gacagcaatt
     3481 ttaatcaggt ttgtggcaac tttttaggat attggtcagc cgatcaagtc tgcggttcaa
     3541 gtaatgtccc agctgccgta cagagttatt ttaaatgcaa tcaacccctc cctacttcta
     3601 caacgccttt ttatccttcc ggcgctgtct tatccaactt gatgctatgc tctgttccca
     3661 caggatttac cggtccaagg tataacacct gttataatgc ttatccttct tcttccccca
     3721 cagacattgc ccaatgttgt gggtgtgcag attggtggaa tcctgctcaa accaacaatg
     3781 tagcaattgg agcaaatcct aataccgaaa gttgtactca gcctggcgcc ttgcagccac
     3841 aaaccaatgc ccagtggaat tcatttgtac aaccaatgat acaatggatg aaacgagctt
     3901 gcccttctgc ttatatttat ccttttgacg acaaaaccag tggatttact tgtactaaca
     3961 acctgtcagg gcaacctaac tccacaagct atatcatcag gttctgtcca ggaggcatca
     4021 cggggcttcc agcaggagta aatgagggac ggggataaaa tacatggccg gtattaacgc
     4081 ttcgtacttt atagttgcaa gaagcgaatc gttagtaccg gtaaacatga acaacaatta
     4141 ctttaatata atatcctgca cttcaaagat gcgtctgtca gcagaaaatt tagcttgaaa
     4201 caaatgaata ccttcctcac gcatcttagg tgccagctca gacaagtacc ttgacaaatt
     4261 atctctattg tccagctggt attgaacagt caatttttcc ttacctgatt ttgtttcttt
     4321 ttcctgcttt agaatgcacg cctgcataaa tcctggaaat tggattattt ccgcaacatg
     4381 ctttttgagc catgcttgaa attgtacaaa tatctccaga tcaatcgtta ggtttacttc
     4441 ataaatcacc ataatttatc ttcccaaatt cccatgattt gaattcacca attgcacaca
     4501 acaactggat ttatttttag aataatgagt catgccatga ttagatacga tataccattt
     4561 agctataaac aaataataaa ttgtatttta atttatactt tagttatttg aattttaaat
     4621 gaccgattat ctgtttgccg ctgatttaca atagta
//
LOCUS       DAXLDT010000213         4373 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXLDT010000213 DAXLDT010000000
VERSION     DAXLDT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.214"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            51..1727
                     /gene="kaiC"
                     /locus_tag="ACF2SP_002860"
     CDS             51..1727
                     /gene="kaiC"
                     /locus_tag="ACF2SP_002860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HFD2360625.1"
                     /translation="MHLLRGRNVNTNKAKTGIKGLDDILNGGYLKNKPTLLKGSPGTG
                     KTIFTLFFTHEQLKSNNSVIYVTCDESPEQILAHMDGFNLESSKFLQEGKLLILDFTP
                     ILTDEVAGKFNINALLLRIEQAQQKSKADTLIIDSLQSLLLGIKNYDPNYELLRLFQW
                     ARQNNLTVLTTMAETQTILQTEIYDEYVVDCAIELKQKVKNDLMTRYLRIIKNRSSSH
                     GTNEYPFSITHNGISLLPITSTKLNTTTSAEYLSTGIKALDRMLGNKGYQTGSQIIIS
                     GRSGTAKTLFAASFAQSAVRQGRKVLFISFEESPNDLIRHVKSINIDLMKYITKKKLH
                     INSRRSVEMGLEDHIISIIELTEEKQFDVLILDPISALLDLGNTMEVKRLFIRFISYM
                     KSLNKTLLFTELIPDYSKELTILGLSSLTETWIRLSNVESNGEFNRLIHIAKARGIKT
                     SNQIKEFYVTDKGINIEAPYLGDNQMMFGSQKSAHILREKQELQQRKEEIKRIELEID
                     KLAETQRAQLKIQEATFLAKRTELIAKKNELIAKEEALIKRMESNKLLRE"
     gene            1732..2004
                     /locus_tag="ACF2SP_002861"
     CDS             1732..2004
                     /locus_tag="ACF2SP_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HFD2360626.1"
                     /translation="MTRTKLKLFVIGNSAISKRAIINLQSICSDPKLADLCDIEVVDL
                     CKNKGIAEQEKILATPILIKKEPLPERRIIGDLSDKQKVISALEMD"
     gene            2013..>4373
                     /locus_tag="ACF2SP_002862"
     CDS             2013..>4373
                     /locus_tag="ACF2SP_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2360627.1"
                     /translation="MISNERINTQNFWYQLVEKMAIGILVINNEGQILYANDYVVQMF
                     DVPHTELVGTNFLYPMAANETQEVEILKSSNEILTAHMTVRKSIWENRQVWIISLFDI
                     TELKLKEKLLKISSKGISSAFEGIIIIDPEGTILEANNAFMKMTGYTEKEVIGQKPVL
                     LHSRKQNEKFYKQLWKILTEKGQWSGELWAKKKNKRLFPAFLTISAVKNSDGDVINYI
                     GYYHDLTLIKKQEQQIESFKYYDYLTGLPNKLLLTQRLQMLINRLSEENKELIIFNIR
                     IFDSNSKNSLLDFLPNIRDKIILEVVKRVDRTSPKKMFFSRIGLSEFIVVYFIKSRIE
                     VMASIAQRMIEKLSCPYHISHKTIILQTIIGLTAYYKNTAFSAEDFLQQSEVARHKAQ
                     IRGINIFEFFDPQVEEQIIEFNKRIESVSKAIMLNELKIYYQPIVELSTAEVAGLEAL
                     LRWKHPKKGLLLPKQFLSDIDNHPLSLQLGDWVLHQVLEQIEKLLACGIKIPIHINVS
                     AYQLQHPNFVKKLFDTLALYPSVPPHLIMLEVLETQAIEDLILVSQIIAECKTKGVNF
                     SLDDFGTGYASLTFLKELNTAEVKIDRSFIIDIFDNPKELVILKTMIEFCRVIGRTLV
                     AEGVETIAHGKLLNLFGCNLIQGYAIAKPIPATKLIPWIKNWKLAPEWGENRFSHAAL
                     NKLVKISIKHYLNFQLIRLYLENDQSPKPQLSLQACPVEQWFLENQQTIKEDRRFKKV
                     CDLHHKEHKLGNIIIQLIQTGQKEQAAKQLVSLDSLRINFLKELMHF"
BASE COUNT         1555 a          803 c          777 g         1238 t
ORIGIN      
        1 ttaaacaatc agaatattat ttatcgcttc aacagtggta ttttacagat atgcatctgt
       61 tgagaggacg aaatgtgaat accaataaag caaaaacggg aattaaggga ttagatgata
      121 ttttaaatgg tggttattta aaaaataaac caacccttct caaaggcagc cctggtacag
      181 gaaaaacaat atttaccctt ttttttacgc atgaacaact taagtcaaac aacagcgtca
      241 tttatgtcac ctgcgatgag tcacctgagc aaattcttgc tcatatggat ggctttaacc
      301 tggaaagcag caaattttta caagaaggca agcttttaat tcttgacttc acacctatat
      361 taacggacga agtggccggg aaattcaaca tcaatgcact tttactccgt atagaacagg
      421 cacaacagaa aagcaaagcc gatacactaa ttattgattc acttcagtcc cttcttttag
      481 gaatcaaaaa ttatgatccc aactacgagt tgttaagatt atttcagtgg gcgcggcaaa
      541 acaatcttac cgttctcaca acgatggccg aaacccaaac gattttacaa acagaaatct
      601 atgacgaata tgttgttgat tgtgctattg agctaaaaca aaaagtcaag aacgacctga
      661 tgacgcgcta tttgcgaatc attaaaaatc gtagttcgtc tcatggcact aatgagtatc
      721 ccttttctat tactcataat ggcatttcat tacttcccat cactagcaca aaactgaata
      781 caacaacttc tgctgaatat ctcagcactg gtataaaagc cctggatagg atgctaggca
      841 ataaaggata ccaaacagga tcgcagataa taatcagcgg acgttcagga acagcaaaaa
      901 cgctttttgc agcaagtttc gcccaatccg ctgttcgaca aggaaggaaa gtgctgttca
      961 tctcatttga agaatcccct aatgatttaa tccgtcatgt caagtccatt aatattgatt
     1021 taatgaaata catcaccaaa aaaaaattac atattaattc aagacgctct gtggaaatgg
     1081 gactggaaga ccatattatt tcaatcatcg aattaacaga agaaaagcaa tttgatgtcc
     1141 tgatactgga tcccatctca gctctgctgg atttaggtaa tacgatggaa gtcaagagac
     1201 tgttcatacg ttttatttct tatatgaaat ctctcaataa aaccctttta tttactgaac
     1261 tgatacccga ttattccaaa gagctaacca tactcggctt atccagctta acagaaacct
     1321 ggattcgctt atctaatgtg gaaagtaatg gagaattcaa tcgattaatc cacattgcca
     1381 aagcgcgtgg cataaaaaca tccaaccaaa tcaaagaatt ttatgtcact gataaaggga
     1441 ttaacatcga agccccttat cttggtgata accagatgat gtttggttct caaaaatcag
     1501 cccacatctt gagagaaaaa caagaattac aacagaggaa agaagaaatt aaacggattg
     1561 aacttgaaat tgataagctt gcagaaacac aacgtgctca actcaagatt caagaggcaa
     1621 cctttttagc taaaaggact gaattgattg ctaaaaaaaa tgaattgatt gctaaagaag
     1681 aggcgctgat taaacgcatg gagtccaaca aattgttaag agagtaaatg aatgactcga
     1741 actaagctaa agctgtttgt gattggaaat agtgctatat caaaacgtgc cattattaat
     1801 ttgcaatcga tatgctcaga tcccaagctt gctgatttgt gcgatatcga ggtagtcgat
     1861 ctatgtaaaa acaaggggat agcagaacaa gaaaaaattc tggcaactcc gatattaatt
     1921 aaaaaagaac ccttgcctga acgtcgaatc atcggtgatt tatccgataa gcaaaaagtt
     1981 atttctgctt tggaaatgga ttaggtgata tcgtgatatc aaacgagcgt ataaacacac
     2041 aaaatttttg gtaccaactc gttgaaaaaa tggctattgg gatattggtg attaataatg
     2101 aagggcaaat tctttatgcg aatgattatg tagtacaaat gtttgatgta ccccatacag
     2161 agcttgttgg caccaatttt ttatatccga tggctgcaaa tgaaacacag gaagttgaaa
     2221 ttttaaaatc cagtaatgaa attttaacag cacatatgac agtcaggaaa agtatctggg
     2281 aaaatcgcca ggtatggatt atttctctct ttgatattac agagctcaaa ctaaaagaaa
     2341 aacttttaaa aatttcaagc aaaggcatca gttcagcatt tgaaggcatt atcatcatcg
     2401 atccggaagg caccattctt gaagccaata acgcgtttat gaaaatgaca gggtatactg
     2461 aaaaagaagt cataggacaa aaaccagtac ttttacactc aaggaaacaa aatgaaaaat
     2521 tttacaaaca actttggaag atattgactg aaaaaggtca gtggagtggt gagctttggg
     2581 ctaaaaagaa aaacaagaga ttatttccag cctttttgac catttcagct gttaaaaact
     2641 ccgatggtga cgtgattaat tacattggtt attatcacga tttaacgtta ataaaaaaac
     2701 aagaacaaca aatcgagagt ttcaaatatt atgactattt aacaggatta ccgaacaaac
     2761 ttttactcac ccaaagactt cagatgctta ttaacagatt atctgaagaa aataaagaac
     2821 tcatcatttt taatatcagg atatttgatt cgaatagcaa aaactctctg ttagattttc
     2881 tcccaaatat ccgagataaa atcatcttgg aggtggttaa gcgtgttgat cgaacatcac
     2941 ctaaaaaaat gtttttctca cgcatcggct taagtgagtt tattgttgtt tattttatca
     3001 aaagtagaat tgaagtgatg gcttccatcg cccagagaat gatagaaaaa ctctcttgcc
     3061 cttatcacat tagtcacaaa actattatct tgcaaacgat cattggacta actgcgtact
     3121 acaaaaatac cgcgttcagc gccgaggatt ttttgcaaca atcagaagtt gcccgccaca
     3181 aagcccaaat caggggtatt aatattttcg agttttttga ccctcaggtt gaagagcaaa
     3241 ttatagaatt taataaacgc atagaatctg tctctaaagc aatcatgtta aatgagttga
     3301 aaatttacta ccaacctatt gtggaattaa gtacagctga agtggcaggt ttggaagctt
     3361 tgctgcgctg gaaacacccc aaaaaaggcc tgttacttcc aaaacaattt ttatctgaca
     3421 tcgataatca ccctctttct ctgcaacttg gagattgggt tctacatcag gtattagagc
     3481 aaattgaaaa actactggca tgcggcataa aaatccccat tcacattaat gtgagcgctt
     3541 atcagctgca acatcccaat tttgtcaaaa aactgtttga cacattggca ctatacccaa
     3601 gcgttccccc tcacctaatc atgcttgaag tccttgaaac acaagccatt gaagatttga
     3661 tattagtatc ccagataata gccgagtgta agaccaaggg agtcaacttt tctttggatg
     3721 attttggaac aggttatgcc tctttaactt ttcttaaaga attgaatact gctgaagtta
     3781 aaattgatcg aagttttatc attgatattt ttgataaccc gaaagaactt gttattctga
     3841 aaacaatgat agaattttgt cgagtcattg gtcgtacctt ggttgcagaa ggggttgaaa
     3901 caatagcaca tgggaaatta ttaaacttgt ttggttgcaa tctaatccaa ggctacgcta
     3961 ttgccaaacc tatccccgct accaaattaa ttccatggat aaaaaactgg aagcttgctc
     4021 cagaatgggg agaaaaccgg ttttcgcatg ccgcattaaa taaattagtc aaaatctcca
     4081 taaaacacta tcttaacttt caattaatca gactttattt agagaatgat caatctccca
     4141 aacctcaatt atcacttcaa gcatgcccag tagagcagtg gtttcttgaa aaccaacaaa
     4201 caatcaaaga ggacaggcga tttaaaaaag tctgtgattt acatcacaag gaacataaat
     4261 tgggcaatat aataattcaa ttaattcaaa cagggcaaaa agaacaagcg gccaaacaac
     4321 tggtaagcct tgatagttta agaattaatt tcttgaaaga gttaatgcat ttc
//
LOCUS       DAXLDT010000214         4362 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXLDT010000214 DAXLDT010000000
VERSION     DAXLDT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.215"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(104..847)
                     /locus_tag="ACF2SP_002863"
     CDS             complement(104..847)
                     /locus_tag="ACF2SP_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HFD2360628.1"
                     /translation="MNNLPPDIMKNIALLISCEHAVNTVPYEYQTLFEPHKNILESHR
                     GIDFGALEIAQSIHKNNHCSLFQATCTRLLVDCNRSINHPHCFSEITKNLSSDKKKKI
                     LDEFYFPFRNRVMDHIHQLISQGLQVWHLSTHSFTPVMNNIIRTTDIGLLYDPRSNSE
                     KILARQWQKEIKILHPQLKVRMNYPYKGISDGFTTSLRKKYSHHEYIGIELEVNQKLV
                     FHNHSLDALKNTLPLSISNTIKAYQKSER"
     gene            complement(828..2048)
                     /locus_tag="ACF2SP_002864"
     CDS             complement(828..2048)
                     /locus_tag="ACF2SP_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HFD2360629.1"
                     /translation="MSDFPIFSVLGIEIEYMLVDKESLNVQPKSDLILRALAGHQVNE
                     IELGDIAISNELVLHVLELKNNGPKPLHVPVAEHFQKTIRELQPLLAQHNLLLLPTGA
                     HPWMDPNTETIRWPHGNHDIYNQFNSIFNCQGHGWANLQSMHVNLPYSNEEEFCQLHN
                     AIRLILPLLPAIAASTPFLDGQATGLLDSRLYFYSRNQQRIPSISGDIIPEFIRTERE
                     YQEQILEPMYRDIGPYDPEGILQYQWLNSRAAIPKFDMKAIEIRILDSQECVEADIAI
                     AKAINAILKYWQSTSNFYLDNPCDTKSLKYVFDETIKNGLSCHINNKKLLEQWQLPLQ
                     GMSVNTIWSLLIERISHELDRKQQLALELILKQGNLSERILKACSSCVNHDSLKQVYT
                     QLAHCLLRNEQFTP"
     gene            complement(2041..3495)
                     /locus_tag="ACF2SP_002865"
     CDS             complement(2041..3495)
                     /locus_tag="ACF2SP_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HFD2360630.1"
                     /translation="MQTVIVTDDLDSWSFLSELAPIVHALEYLSGDEYHQNKSFRVIN
                     LCRSYNYQSIGYYVSLLAQARDHKALPSVHSIQDVLNVSLSKLISQDTHEEIQHSLHD
                     IKGNEFVLSLYFGQNLAKRYATLAKQLHGLFPLPLIRFYLEKKKQWRIKTVVPLSLSD
                     VPEHHLEFMRQSAENYLSKKRVHQWRKKQRFHDLAILVDPSEPNAPSNKKALELFVSC
                     GEELGLNVDIIDKNEAKAIAEYDALFIRATTSVNHYTYRVSRRAAQENLVVIDDPQSI
                     IKCSNKVYLAELLSSHQIMTPDTLFISKYDKELPKIEFPCVLKKPDSAFSHGVVKIDD
                     EKSLQKSLNQFFKNSDLVVVQPFIPTEFDWRIGILDNKALFACRYFMAKGHWQIYDWH
                     CQEENKEGDSETLPLHEVPEQIIKIALKSTRLIGDGLYGVDIKSHGNKYYVIEVNDNP
                     NIDHGLEDKILGQNLYEQIMSVFLQRIRRKHGYV"
     gene            complement(3499..4212)
                     /locus_tag="ACF2SP_002866"
     CDS             complement(3499..4212)
                     /locus_tag="ACF2SP_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFD2360631.1"
                     /translation="MIDLIIQTQPDDETCGPTSLHAIYLYYGLDLPLNEIIQGVDKSL
                     SGGTLTPMLGKHALLHGFRTTVYIYNLNIFDLTWFHNGEVNNSFLMDKLEAQMKYKND
                     PYITKASLAYLDYLKLGGTIRSKSLNVDLLKNYFNKKIPILTGLSATYLYRSAREYFT
                     PEGKSVYDDIRGEPCGHFVVLCGYDVKKRLVIVADPHAENPLSHNNYYKVSINRLINS
                     IMLGVLTYDANLLIIEPKE"
BASE COUNT         1294 a          760 c          873 g         1435 t
ORIGIN      
        1 acacatgatc agtgcttgta aaaactcaat cttttttgta aaaaaaattt ttaattcagc
       61 cacttggttt ttttaaattg caacttgaaa aaaatgttgt cgcttacctc tctgattttt
      121 gataagcctt aatcgtgttt gatatgctca atggtagtgt atttttgagt gcgtccaggc
      181 tgtgattgtg aaagacaagt ttttgattga cttccagttc aatgcctatg tattcatgat
      241 gactgtattt tttacgcaag gatgtagtaa atccatcact gattccttta taaggataat
      301 tcatacgtac ttttaactgg gggtgaagta ttttgatttc tttttgccat tgcctggcca
      361 atattttttc tgaattactc cgggggtcat atagaaggcc aatgtcagtc gtgcgaatga
      421 tgttgttcat tacaggagta aaactgtgtg tggacagatg ccaaacttgc agtccttggc
      481 taatcagttg gtgaatgtgg tccatgacgc gattgcgaaa cggaaaataa aattcatcca
      541 gtattttttt ctttttgtct gatgataaat tttttgtaat ctcggaaaaa caatgaggat
      601 gattgatact cctattgcaa tcaaccaaga gccttgtaca ggtggcttga aacaaactgc
      661 aatggttgtt tttatgaatc gattgtgcaa tttctagcgc accaaaatca attcctctat
      721 gagattctag tatgttttta tgaggttcaa acagggtttg atattcatag ggaactgtat
      781 tgacagcatg ttcacaactg attagaaggg ctatattttt cattatatca gggggtaaat
      841 tgttcattac gaagcaaaca atgtgctaat tgcgtataca cttgttttaa agaatcatga
      901 ttgacacagg agctacaagc cttgaggatt ctttcactca aatttccctg ttttaatata
      961 agctccagtg ccagttgctg tttcctatcc agttcatgac taattctttc gatcaatagt
     1021 gaccaaatgg tattgactga catcccttga aggggtaatt gccattgctc cagtaatttt
     1081 ttgttattga tatgacaact taatccgttt tttatggttt catcaaaaac gtattttaaa
     1141 ctcttggtgt cgcatggatt atcaagataa aaattcgagg tgctttgcca gtattttaaa
     1201 atggcattga ttgcctttgc tatggcaatg tctgcttcta cacattcctg gctgtcaaga
     1261 atgcgaattt caatagcctt catgtcaaac ttgggaatcg cggcacgaga gtttagccat
     1321 tgatattgta atatcccttc gggatcgtaa gggccaatgt ctcgatacat gggttcgaga
     1381 atttgttcct ggtattccct ttctgttcta ataaattcag gaataatatc gccgctgatg
     1441 gagggaatgc gttgctgatt gcggctataa aagtacaagc gggaatccag taatcctgta
     1501 gcttggccat caaggaatgg agtacttgct gcaatggcgg gtaataaagg taaaatcaag
     1561 cgtatggcgt tatgcaattg acaaaattct tcttcattgg aataaggcag gttgacatgc
     1621 atactttgca agtttgccca gccatgtccc tggcaattga aaattgaatt aaattgatta
     1681 tagatatcat ggttaccatg aggccagcga attgtttcag tatttgggtc catccaggga
     1741 tgagcgccag taggtaatag caataaattg tgttgagcaa gaaggggctg gagctctcta
     1801 atggttttct ggaaatgttc agctactgga acatgtagtg gtttgggacc attatttttt
     1861 aactcaagga catgcaatac taattcattg cttatcgcaa tgtctcctaa ttctatttca
     1921 ttaacctgat gacctgccaa agcccttaga ataagatcgc ttttaggttg gacatttaaa
     1981 ctctctttat ccaccagcat atattctatt tcaatcccta agacagaaaa aataggaaaa
     2041 tcagacataa ccatgtttcc tgcgaattcg ttgtaagaag acactcatga tctgctcata
     2101 cagattttgg cctagtattt tatcttcaag gccatgatca atattgggat tatcgttcac
     2161 ttctataacg taatatttat tgccatgact ttttatatcg acaccatata atccgtcacc
     2221 aatcaaacgt gtacttttca gagctatttt aataatctgc tctggcactt catgaagggg
     2281 tagtgtttca ctatccccct ctttattttc ttcctggcaa tgccaatcat agatttgcca
     2341 gtgacctttg gccataaaat agcggcaggc aaaaagtgct ttattatcaa ggatgccgat
     2401 gcgccaatca aattcagtcg gtataaaagg ttgaaccact accaaatcag aatttttaaa
     2461 aaactggtta agagattttt gtaaagattt ttcatcatct attttaacca cgccatgaga
     2521 aaaggcgctg tcaggttttt ttaaaacaca ggggaattca atcttgggta attccttatc
     2581 atatttgctg atgaataagg tgtctggcgt catgatttga tgactgctta agagctctgc
     2641 aagataaact ttgttggagc atttaattat agattgtggg tcatcaataa cgaccaaatt
     2701 ctcctgggct gcgcgtcgtg acacgcgata agtataatga ttaacggaag tagttgctct
     2761 gataaaaaga gcgtcgtatt cagcgattgc ttttgcttca tttttatcta tgatgtcaac
     2821 gtttaatccc agttcctcac cacaagaaac aaaaagttca agggcttttt tattggatgg
     2881 ggcattaggt tcggaggggt cgacaagaat ggccaaatca tgaaatcgtt gttttttacg
     2941 ccattgatga actctctttt ttgataaata attttcagct gattggcgca taaactccag
     3001 atgatgctct ggtacatccg acaaagacaa aggaactact gtcttaatcc gccattgctt
     3061 tttcttttcc agataaaagc gaatgagtgg taaaggaaaa agaccgtgga gttgttttgc
     3121 caatgttgca tatcgttttg ccaggttttg accaaaatag aggcttaaaa caaattcatt
     3181 gcctttgata tcgtggagac tgtgttgtat ttcttcatga gtatcctgag agatcagttt
     3241 ggataaactg acgtttaaaa catcctgaat actgtgtaca gagggtaatg ctttgtgatc
     3301 gcgggcttgt gcaagtaaag atacataata gccaatcgat tggtaattat aagatcggca
     3361 taagttaatg acccgaaatg atttattttg atgatactca tccccactaa gatattccaa
     3421 ggcatgtact atcggggcaa gttcacttaa aaaagaccag ctatctagat catcagtaac
     3481 aataactgtt tgcatttatc actctttagg ttcaattatg agtagattcg catcataagt
     3541 tagcacacca agcattatag agttaatcaa gcgattgatg cttactttat aataattgtt
     3601 atgagacagt gggttttctg catgggggtc agcaacaatt acaaggcgtt tttttacgtc
     3661 ataaccgcac agtacaacaa aatgcccaca aggctcacca cgaatatcat cgtaaactga
     3721 tttgccttcc ggagtaaagt attctcttgc actgcgatag aggtaagtag cacttaagcc
     3781 agttaaaatg gggattttct tattaaaata attttttaac aagtccacgt tcagtgattt
     3841 ggagcgaatt gttcctccca gttttaagta gtctaaatag gccaaagaag ccttggtgat
     3901 ataagggtca tttttgtatt tcatttgagc ttcaagttta tccatcaaaa aggagttatt
     3961 gacttcacca ttatgaaacc aggtgaggtc aaatatattc aggttataga tgtaaacagt
     4021 tgttctaaat ccatgaagaa gggcatgttt tcccaacata ggtgttaagg tgcctcctga
     4081 aagagatttg tccactcctt gaatgatttc gtttaatggc aaatccaaac cgtagtataa
     4141 ataaatggca tgtaagctgg ttggtccgca agtttcgtca tcaggttgag tttgtattat
     4201 caggtcaatc ataggcaaat ccttgcggta aaaatagcgc catcatgatt taaaattaga
     4261 catgagtggc ttctttgtca aatggtgtaa tcaattcaat aataagtgat tttagaagac
     4321 tgataatcat gtgatattta agaaaatgag attgaaaaaa ca
//
LOCUS       DAXLDT010000215         4320 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXLDT010000215 DAXLDT010000000
VERSION     DAXLDT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.216"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            361..1053
                     /locus_tag="ACF2SP_002867"
     CDS             361..1053
                     /locus_tag="ACF2SP_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2360632.1"
                     /translation="MKDKSILLVEDNVKLANYLKESLQEAGYDVSIEKRGDRAVYRII
                     REQPCLVILDIMLPGMNGDQICHTIRDEYLGKILMLTAINDIESEVSSLNLGADDYLT
                     KPVADEVLKARIEALLRRPNLVNNQNQFQFGNFSINFSTKSVHLFDEEISISTSDFEM
                     LALLVKNHDRLLSRDSIMYALSGHEYDGVDRGIDLKISRLRKALNDNNKKPYRIKTIH
                     KKGYIFVSAAWE"
     gene            1293..3116
                     /locus_tag="ACF2SP_002868"
     CDS             1293..3116
                     /locus_tag="ACF2SP_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2360633.1"
                     /translation="MKDAAIEILIYKPDPDHPLPENQRGVVINPHLKQRFKQFVPIGV
                     YLEGSDLKYYFFDQCDKSPKIYSSLDELKRDLPKELDNIFEKNPKLFIMGHAGGGEYG
                     MGNCHGPSEQLYDDNFDNLLHGFKRALPAHHGEVFVTLEGCNTDNQLNAAANSQEKTF
                     LERVSVKHPEITFGGTGPWNAQDVQTGFRSLSPASPITSMAGNIWKAGNSVIFHHGEY
                     QVAVRKSLFASTQTAKELKVNTIEYARAILGKDGADELIAKIALNRDILNIQDLKKIE
                     GFPELRFEGEDVAKFVEQENQILGKEQENYLTHVRDILGRADPIEQLTDRDVLELLLG
                     LKEPSVFKGHEDLLESILANKALLNLAMVSCGKVLIGNPSNDSVIALLLKHGADINSA
                     DKKGMTALHYAVQNFYNYRKEPLHLIKKLLECGASLEVRNEKGQTPIETAQEHSKDGR
                     VTASDKLLASLKSSNSPPTPIELKQSVRQIFQMSQHKADELLSLPIAKREYSKCSEGT
                     LYIPEDAVEKLHGILQSATGSNELLSAFESELSVVISALEKGIGTGEAIDGEEDEFIP
                     KRFENAKLQLTAVIDALVGMKQEMDTSKDTTVEDQLPKLKS"
     gene            complement(3120..>4320)
                     /locus_tag="ACF2SP_002869"
     CDS             complement(3120..>4320)
                     /locus_tag="ACF2SP_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD2360634.1"
                     /translation="PGEIIFNYMNPVLKQVVQHLLSKSKNTWSNELLHLEKIYGFPLH
                     VYKTKSKYLPGNIINSLSTKRLVFETNKNSSQIVILYYRFSGGILKIGPLSYLPVMAR
                     ISDVMYYFIGTFFFISLCLIALFSLLFVRNMKKVYQITKNFSQGNFDFHRKIGTTSVL
                     YGLYRNIIQMGEQLKELIESHKQLCRFVAHEIRTPLSTIQMATDSIKRKNAEDALLNK
                     QINSIQEDIADINRIVSTFLIYSKMHSNELKLKQSETDIIVWLRKLLESYSSSTFEIT
                     FYSNELNSLKAYIDENILKHAVTNLITNAMKFAEHTISLTISLDNSHILLHVDDDGPG
                     LPEEGADDIFSEYTIAEDAGIGDKHIGLGLAIVKKVVNLHGGKVMATQSPILKGARFT
                     IVLPIYS"
BASE COUNT         1476 a          697 c          803 g         1344 t
ORIGIN      
        1 ggaataaatt aaaaaatttt cttcgaaaat gagaacgttt ttcgggatag caatgctcac
       61 aatgtttatt gtccatatat gctgtgggaa caggttttaa tgatttgcaa atgtcacgtg
      121 aagacttgat ttctttatgt tcatcaaaat ttttaacata agcgctcata atttatctcc
      181 acagattcgc ccattagcta tcattatatc gagttaaaac agatcaattg tatataaaat
      241 gtacggatgc gccgtgtgtt tgcttaccta ctgatatcta tgtaacactg aacttaaaaa
      301 tcaattcaga ctaaatttgt ttagagagtt ttcttattta aagaggctta gacagaacga
      361 atgaaagata aatcaatatt gcttgtagaa gataacgtta agttagccaa ttacctcaaa
      421 gaaagcttac aagaagcagg ttatgatgtt tctattgaaa aacgtggtga tagagcggtc
      481 tatcgcatca ttcgtgagca accttgccta gtcatattag atataatgct tcctggcatg
      541 aatggagatc aaatatgcca taccattaga gatgagtatt tggggaaaat actcatgcta
      601 acagcaatta atgatattga aagtgaagta tcctctttaa atttaggagc agatgactac
      661 ttaactaaac ctgttgcaga tgaggttttg aaagcaagaa tagaggcatt gttacgtcga
      721 cccaatttag ttaataatca aaatcaattt caatttggca atttttctat caattttagt
      781 actaagagtg ttcatctctt tgatgaagaa atttcaataa gcaccagtga ctttgaaatg
      841 cttgctttgt tggttaagaa ccatgatagg ttgctgagtc gagatagcat tatgtatgct
      901 ctttctgggc atgaatatga tggggttgat agaggtatcg atttaaaaat atcacgttta
      961 agaaaagcat tgaatgataa taataaaaag ccctatcgta taaaaacaat ccataaaaaa
     1021 ggatatatat ttgtatcagc ggcttgggaa taaatacaga tcacagccac cgatcttatg
     1081 taagacttat gaaaaatagt aaaaaattct tttgcctcga tcttgagatt tttcgtccag
     1141 ttatgaaagc ttcaccaaat atatcgcaaa tctataatca tttgccctcg acaaaagtat
     1201 cattttagaa cgtttgtaca atatatatac atttgagccg agtgtttttg ataaaatcaa
     1261 cctattattt taagtaatag tcgagatgag tcatgaaaga cgctgcaata gaaatcttaa
     1321 tatataaacc agatccagat catccactgc ctgaaaatca gagaggtgtg gtgataaatc
     1381 ctcacttaaa acaacggttc aagcaatttg ttccaattgg cgtctattta gaaggctctg
     1441 atttgaagta ttattttttc gatcagtgtg ataaatcccc aaaaatctat agtagtttgg
     1501 atgaactcaa acgagactta ccgaaagaac tagacaatat atttgagaag aacccaaaat
     1561 tatttattat gggccatgct ggtggtggcg aatatggtat gggtaattgc catggcccaa
     1621 gtgagcagtt atatgatgat aattttgata atttattaca tggttttaaa cgagcattgc
     1681 ccgcacatca tggtgaagtt tttgtgactt tagaaggttg taatacagac aatcaactta
     1741 atgctgcagc aaactcacag gaaaaaacat ttttagaaag agtatcagtg aaacatccag
     1801 aaatcacgtt tggtggaact ggaccatgga atgcgcaaga tgtacagacc ggcttccgat
     1861 ccctttcgcc ggcatcccct atcacatcga tggcaggaaa tatttggaag gctggaaaca
     1921 gtgttatttt tcatcatggt gaatatcaag ttgccgttcg aaaatcttta tttgcctcaa
     1981 cacaaacagc taaagaacta aaagttaaca ccatagaata tgcccgtgca attctaggta
     2041 aagacggagc cgatgaactg atagcaaaaa tcgctttaaa tcgagatatt ctaaacattc
     2101 aagatttaaa gaaaattgag ggttttccag aattgcgatt tgaaggagaa gatgttgcaa
     2161 agtttgttga acaggaaaat caaatattag gtaaagaaca agaaaattat ttaacacatg
     2221 tccgtgatat cttaggtcgg gcggacccta tagaacaact gacagataga gatgtactag
     2281 aacttctctt aggtttaaaa gaaccttctg tatttaaagg tcatgaagac ttgctagagt
     2341 caattctagc taataaagct ttgttaaacc tggcaatggt aagttgtgga aaagtcctta
     2401 ttggtaaccc aagtaatgat agcgtcattg ctttattatt aaaacatggc gctgatatta
     2461 atagtgctga taaaaaaggc atgacggctt tgcactatgc ggtacagaat ttttataatt
     2521 atagaaaaga gccgttacat ctcattaaaa agttattaga gtgtggtgct agtcttgaag
     2581 tgcgaaatga aaaaggacag actcctatcg agacagcgca agaacatagc aaagatggaa
     2641 gagtaactgc tagtgataag cttctcgcat ctttgaaatc tagcaattcg ccaccaactc
     2701 ccatagaact aaaacaatct gttagacaaa tatttcagat gagtcaacac aaggctgatg
     2761 aactactttc attacctatt gcgaaacgtg aatatagcaa atgcagtgag ggcactcttt
     2821 atattcctga agatgcagtg gaaaaattgc atggaatctt gcaatctgca acggggagta
     2881 atgaactatt atcggcgttt gaatcagagc ttagtgtggt tatcagcgct cttgaaaaag
     2941 gcattggaac tggagaagcg attgatggtg aggaagatga atttatccct aaacgttttg
     3001 aaaatgcaaa actccagtta acagcggtta tcgacgcact ggttgggatg aaacaagaga
     3061 tggatacttc aaaagatact acagttgagg accaacttcc aaaactaaaa tcttaatcgt
     3121 taagaatata ttggaagtac tatggtgaat cgagcacctt ttaatatcgg tgattgtgta
     3181 gccatgacct ttcctccgtg gagattaaca acttttttaa caatagctaa tcctaaccca
     3241 atatgtttat ctccaattcc tgcgtcttca gcaattgtat attcagaaaa aatgtcatcc
     3301 gcaccctcct ccggcaagcc tgggccatca tcgtctacgt gaagtaaaat atgactatta
     3361 tccagcgaaa tggttaaaga aatagtgtgc tctgcaaatt tcatggcatt tgtaatcaaa
     3421 ttggtaacag cgtgttttaa aatattttca tctatgtatg ctttcaaaga atttaactca
     3481 ttagaataaa atgtaatttc aaatgttgag gaagaataag actctaataa ctttcttaac
     3541 catactatta tatcggtctc agactgtttt aatttcaatt cgtttgaatg catttttgag
     3601 taaatcaaaa aagtgctgac gatgcgattt atgtctgcaa tatcttcttg tatgctgttt
     3661 atctgtttat tgagcaatgc atcttcagcg tttttccttt tgatactatc cgtcgccatt
     3721 tgtatggttg acaaaggggt cctaatttca tgcgcgacaa accggcacaa ttgtttatgt
     3781 gattcaatca gttcttttaa ttgctcaccc atttggatga tatttctata cagcccatac
     3841 aaaacagagg tagtacctat tttccgatga aaatcaaagt tcccttgact gaaattttta
     3901 gttatctgat acaccttttt catatttcta acaaaaagca atgaaaataa ggcaataaga
     3961 caaagggata taaaaaagaa agttcctata aaataataca taacatcact gattcttgcc
     4021 ataactggca gatagcttag tgggccaatt ttgagtattc caccactaaa acgataatag
     4081 agaataacaa tttgtgacga gtttttattt gtttcaaata ctagacgctt tgttgataaa
     4141 gaattaatta tattacctgg taagtattta cttttagttt tataaacatg aagagggaaa
     4201 ccatatattt tttctaaatg taatagttca ttactccagg tattttttga ttttgataat
     4261 aaatgttgaa cgacttgttt taaaacagga ttcatataat taaaaataat ttcgccagga
//
LOCUS       DAXLDT010000216         4309 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXLDT010000216 DAXLDT010000000
VERSION     DAXLDT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.217"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            88..375
                     /locus_tag="ACF2SP_002870"
     CDS             88..375
                     /locus_tag="ACF2SP_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360635.1"
                     /translation="MSAVMQQVTQGNDALSHQVISAVKGYLTSVGSKDANLNLYQLIV
                     EEVEAPLFRTVMELTRYNQSKAARVLGVSRGTLRTKLKRYFDDEFIGTRDF"
     gene            486..858
                     /gene="rnpB"
                     /locus_tag="ACF2SP_002871"
     ncRNA           486..858
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACF2SP_002871"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(947..1735)
                     /locus_tag="ACF2SP_002872"
     CDS             complement(947..1735)
                     /locus_tag="ACF2SP_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HFD2360636.1"
                     /translation="MKKLIYISLIFVISNAFAQPAATVLFAKQKVISLREGKEYSLSR
                     GSQLEPGDTVVTGAGALINIKYKNGTLVNIGENSRYKILAFSPNPSDVQIKAELSQGK
                     IKFKTTGKSNESLKTPVIAMAITGTSAEIFVKSDKQTYLNLLEGNINNYTPGSYIATP
                     QGVVAGAFPAEGQVNTPAGTDGSISDAETTETISSESADATDTADATDVADATDTADS
                     AQEASDLVGFIESAQVVDQATTDSLEEEIASEIAEISLECISVI"
     gene            complement(1755..2216)
                     /locus_tag="ACF2SP_002873"
     CDS             complement(1755..2216)
                     /locus_tag="ACF2SP_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360637.1"
                     /translation="MKTTRLFMLSMIAHFLLTCSVIHDVWAEKTLNPEKLANTTITLI
                     SNNQNSSTGNCSLQSVTSCTILLHLANPGTPGRLYVTNHSFVTATNIEAVLPSSLESL
                     VNMVEIGCDEVPPGGTCTIYFYPISGPISPQPVQLIGDNIRTATFYLGIVM"
     gene            complement(2256..>4309)
                     /locus_tag="ACF2SP_002874"
     CDS             complement(2256..>4309)
                     /locus_tag="ACF2SP_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HFD2360638.1"
                     /translation="ENLKVIIIDIDEKSLEQEGRWPWPRNKIAELLNKLQENGVVTVG
                     FDIVMSEAETNYALGLIEKLSQFSGKMTTDQKQLIATLNSVANQVDNDQTLVSALGKN
                     NAILGFFFHNSEGIRKGSLPTPLRDTDGNPLLANKLSVYQFTNFNSNLAAFTAAATSG
                     GFVTNLPDNDGVVRHALLLANYKNNLYPSLSLAIAMNYLLTDKVALKKYNNQLTGIEL
                     DGLQIPTNRKGQILIPFWGGPGTLNYYSATDILNNQFNPEELAGSIALVGSSITLLSD
                     LHHTPVAQLFPGVEIVGNIVKGLVEQKIPREFDWESLQGKLCLVIFGLLFALLFSSIG
                     IFGKLLIFLITTIAILTNTVILFVFYNNYIPPAFLLTITTLQTFVNFSYSYVIEKRQK
                     RRISELFGQYVPKEYVKELIEHPEHHTMEGLNREMTVLFTDIRNFTTISETLGAGGVK
                     LLLNSFFTPMTKIIFEHRGTIDKYVGDMIVAFWGAPIEDKEHASHAVLCSLSCIEHLP
                     QINTHLLEHGLPQVNIGVGIATGPMNVGDMGSEFRRAYTVLGDTVNLASRLESLTKFY
                     QVNILVSDKTRSHLDSILWRTVDKVAVKGRKSPLTIYQPLGIVEQASARLLQEQEKYE
                     TALAHYYNQDWLVAEILFNELKNVNPKIYLYQMYLERIAAFKKRGPSPDWDGVYVHRS
                     K"
BASE COUNT         1235 a          826 c          873 g         1375 t
ORIGIN      
        1 tttgatatta tatcggtcat ggtgttagag ccaaattaat gtgctggaca tgatttatgt
       61 tcattaaatt tgtattagga gattaaaatg agtgcagtta tgcaacaagt gacacaaggt
      121 aacgatgcct tgtcacatca agtgattagt gcagttaaag gttatttaac aagtgtcggt
      181 agtaaagatg ctaatttaaa cttatatcaa ttgattgttg aggaagtaga agccccttta
      241 ttccggactg ttatggaatt aactcgctat aatcaatcaa aagctgctag agtgcttggt
      301 gtaagtcgtg gtactttgcg cactaaatta aagcgttatt ttgatgatga attcattggt
      361 actcgtgatt tttaattcag ttatatagct aatgctttcc aagttcagtt atttttcgcg
      421 gtattctaat agaaattcat cgttaattat ggcctttgcc tgggcactct gctaatattg
      481 aagtcagagt cggtcaggca atcgctcttt gcctagcagc aaggggagga aagtccgggc
      541 tccatagggc agagtgccag gtaactcctg ggaggtgtga gcctacggaa agtgccacag
      601 aaaatatacc gcccactttt cctttgggaa tcgtaggtaa gggtgaaatg gtgcggtaag
      661 agcgcaccgc gcgattggta acagtcgtgg cagggaaaac cccactcgga gcaagaccaa
      721 ataggaatcc atttgactgc aaagtcataa cgtatggccc atacgggatt cgggtaggtc
      781 gcttgaggca tgcggtgacg catgtcccag atgaatggtt gcccacgaca gaacccggct
      841 tatcgaccga ctctaatcca tattctatgt caatacatac cattatatgc ctttatcccc
      901 tatataaata agggggtaaa gtcgcataca aaaatgacat gccaagctat ataacactta
      961 tacactccag agagatctcg gctatttcac ttgcaatttc ttcttctaat gaatcagtag
     1021 tcgcttgatc cactacttga gctgattcaa taaatccaac aagatcagac gcctcttgag
     1081 cgctatcggc ggtatccgtt gcatcggcca cgtctgtagc atctgctgta tctgtagcat
     1141 ctgcagactc tgaggagata gtctcagtgg tttcagcatc agagatgctc ccatctgtgc
     1201 cagccggagt gtttacctgg ccctcagcag gaaaggcacc tgctacaact ccctgtggcg
     1261 tagcaatgta actcccagga gtataattat ttatatttcc ttctagcaaa tttaaataag
     1321 tttgtttatc agattttaca aatatttcgg cagatgtgcc tgtaattgcc atcgctatca
     1381 ctggggtttt cagactttca tttgatttgc ctgtggtttt aaactttatc tttccttgag
     1441 ataattcagc tttaatttga acatcagatg gatttgggga aaaggctaga attttatatc
     1501 tggagttctc acctatattt accaaggtcc catttttata tttgatgtta atcagggcgc
     1561 ctgccccggt aacaacagtg tctcctggct ccaattgaga gcctcgagag agtgagtatt
     1621 ctttaccttc tctcagtgag atgacttttt gtttggcaaa taatactgtg gctgctggtt
     1681 gagcaaacgc attgcttata acaaaaatta gactaatata aatcaatttt ttcatgagtt
     1741 cttcctactg gatatcacat cactatacca agataaaagg tcgcagttct tatattgtcg
     1801 ccaattaact gaactggttg aggagatata gggccactta taggatagaa ataaattgta
     1861 caggttccac ctggtggaac ttcatcacaa ccgatttcga ccatattcac aagagactct
     1921 agtgagctag ggagtacagc ttcaatattg gttgctgtaa caaaagagtg gttagttaca
     1981 tagagtcttc ctggggtacc agggttagca agatgaagaa gtatggtaca acttgtgact
     2041 gattgaagtg agcaattgcc agttgaggaa ttttgattat tagaaattaa tgtgattgtg
     2101 gtatttgcca gtttttcggg atttagggtt ttttcagccc aaacgtcatg aattactgag
     2161 caggttaata aaaaatgtgc aatcattgat aacataaaca gcctagttgt tttcataatc
     2221 cttcctattg cagtatttaa aatttctttt tgatctcact tgcttctatg aacatacacc
     2281 ccatcccagt caggtgaagg ccctcttttt ttgaatgccg ctattctttc caaatacatt
     2341 tggtacaaat aaattttagg gtttacattt tttaactcgt tgaagagaat ctcagcgaca
     2401 agccaatcct gattgtaata gtgagctaat gccgtttcgt atttttcctg ctcctgcaga
     2461 agtcttgcag aggcttgttc aacaatacct agaggctggt aaatagtcaa tggactttta
     2521 cgacctttga ccgccacttt gtctacggta cgccataata tgctatccag gtgagagcga
     2581 gttttgtcac ttaccaggat attcacttga tagaatttgg ttagactttc tagcctggat
     2641 gcgaggttaa cggtatcgcc aagaacagta taggctcgac gaaactcaga acccatgtca
     2701 ccgacgttca taggaccagt tgctatacct actccaatat taacttgtgg cagtccatgt
     2761 tccaataaat gggtattaat ttgaggcaag tgctcaatgc aggaaagaga acacaaaacg
     2821 gcgtgacttg catgctcctt gtcttctatt ggtgcccccc aaaatgctac tatcatgtcc
     2881 ccaacgtatt tatcaatggt tcctctatgc tcaaaaatga ttttggtcat tggggtgaaa
     2941 aatgaattta acaacagttt tacaccgcct gcaccaagtg tttcgctgat tgttgtgaaa
     3001 ttacgtatgt ctgtaaacag aactgtcatt tcacgattca acccttccat agtgtggtgt
     3061 tcgggatgct ctattaattc tttgacgtat tctttgggta cgtattgacc gaacaattca
     3121 cttattcgtc ttttttgcct cttttcaatg acatatgaat aggaaaaatt aacaaaagtt
     3181 tgcaaggtag tgatggttaa taaaaatgca ggggggatgt aattgttgta aaatacgaac
     3241 aggataactg tatttgttaa tattgctatg gttgttatta aaaagattaa taatttgcca
     3301 aaaataccaa tagaagaaaa taaaagcgca aataataagc caaaaatcac taaacaaagt
     3361 tttccttgca atgattccca gtcaaattct cggggaattt tttgttccac cagtcctttt
     3421 acgatattac ccactatttc aaccccagga aataattggg caactggtgt atggtgtaaa
     3481 tcagataaga gtgttatgga tgagccaact aaggctatac tcccagccaa ttcctcggga
     3541 ttgaattggt tgttcaatat gtcagtcgct gaatagtaat ttagtgttcc tgggcctccc
     3601 caaaatggaa taagaatttg accttttcta tttgtgggga tttgtagccc atctaattct
     3661 atacctgtca attggttgtt atattttttc agagcaacct tgtcagttaa gaggtaattc
     3721 attgctattg ccaaagataa actgggatag aggttgtttt tataattggc caataaaagt
     3781 gcatgtctta caaccccatc attgtcgggt aaatttgtaa caaacccgcc agaagttgca
     3841 gccgcagtaa atgcggccaa attactgttg aaattagtaa attgatacac tgacagctta
     3901 tttgccagga ggggattgcc gtcagtatct cttaatggtg tcggtaatga cccttttcta
     3961 atgccttcgc tattatgaaa aaaaaaacca agtattgcat tatttttgcc taaagcgcta
     4021 acaagcgttt gatcattatc aacctgattt gcaacgctgt ttaatgtggc aatcaactgt
     4081 ttttgatcag ttgtcatttt tccagaaaat tgactcagtt tttctatgag gcccaaagca
     4141 taattcgttt ccgcttctga catgactata tcgaaaccaa cagtgaccac accattttct
     4201 tgtaatttat ttaataattc agctatttta tttcttggcc atggccatct tccttcttgt
     4261 tctaatgatt tctcatcaat gtctatgata ataactttaa gattttcag
//
LOCUS       DAXLDT010000217         4275 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXLDT010000217 DAXLDT010000000
VERSION     DAXLDT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.218"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            70..597
                     /locus_tag="ACF2SP_002875"
     CDS             70..597
                     /locus_tag="ACF2SP_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFD2360639.1"
                     /translation="MNRIKLGISNLIFMGRWLQLPLYLGLILILAGYVYRFIIELFEL
                     IFHLKGLDDTHIMLGVLDLIDVVMIANLLIMVVMGGYETFVSHLQLDSHPDQPEWLDH
                     FDAGAMKIKLALSLIGISSIHLLRTFIDPAKLSNYSIMWQVVIHLTLIVSALAIALTN
                     RLMDQPARKNLQHPD"
     gene            834..2051
                     /gene="legC2"
                     /locus_tag="ACF2SP_002876"
     CDS             834..2051
                     /gene="legC2"
                     /locus_tag="ACF2SP_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC2/YlfB"
                     /protein_id="HFD2360640.1"
                     /translation="MTDTPKANLSVDEIKEDTPKDQPLPEENTEILVKKLKVLETTQE
                     NFDQIKKHIAAVIDSLAKNRSFFGRVAAYWGEMQLWLKITLGIVLVVPTLTLGIAIQV
                     ASLIVVSVLTLVTYVGSALLLENHVQKEEHITDRLKEEMIGLADNLGKVIESFEPLRK
                     QIAEQIEYFHKENDRLNLNVTQLSDHIMRLTKQAEQLQKTEQTLRKVREELEKTSETL
                     DEKVQVQTELLQINQEELSRIREEIAKNEIELSEKISDLDQVKKEMSVEIEKAETVTQ
                     VLKNTVEKLSQTAIADHNHRESFQNKLNDFLSNKEQSFNDVAQRICEAERELCLVKEE
                     LNFSNKRYQELLERQEEVVKRLEQLQLSRKPTPYSNAKVLGKLGIMATEEKEYPTPVS
                     PEENTIPTQTLTG"
     gene            2373..2687
                     /locus_tag="ACF2SP_002877"
     CDS             2373..2687
                     /locus_tag="ACF2SP_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360641.1"
                     /translation="MSTTKWNLSISRAVIFVIALFASMICARAHFVGGIHTNAGYVGW
                     YHPDYYNHDVIGVGNYHPGYGWVAPVYATPTYIIGNTGYDCQTVQQCDSEGNCIQSQN
                     CD"
     gene            complement(2852..3961)
                     /locus_tag="ACF2SP_002878"
     CDS             complement(2852..3961)
                     /locus_tag="ACF2SP_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360642.1"
                     /translation="MKLKRQCINGVACVFLSLLAALTYPLFAEDNRQFVTGQSQLSAV
                     QDPAESSWLPDIIGLPNEEQVESLTRHVAEVMTEEEDKNCTAIDYNLRMLVFQNFVIY
                     QHMANAQHHYFKEKMVLKYAHVLAMILKESSGDPTNVTDMNGRSISTSKPGTNLQRWS
                     NLLKLTMQNGIKFNYQTNFGLTQLSADRLFVAFKLANGASHSKDFLEGKNGDETPNRV
                     DMDSAIAIRRLIWFYQDIAQGRLKQKDGCINEEDIYKPEFNERYQAGLRMALLYCGTK
                     FLFRVDNEPVWVNENSTFEKAMASIAYCKLGNSKTGYGTDEMDEQCFAEWVTLCPALN
                     VNVALLTPLSYFQTRKNKPVCTDTFNRLLNKEPKN"
BASE COUNT         1424 a          793 c          757 g         1301 t
ORIGIN      
        1 taacaaaccc ttcaggaaag cttatcataa gagcattcaa taagttatat tttgtagtga
       61 atcgacaaaa tgaatcgaat caaacttggc atcagtaatc ttatttttat gggaagatgg
      121 cttcaacttc ccttgtatct cggtttgatc ttgatactgg ccggatatgt gtatcgcttc
      181 atcatcgaat tatttgaact catttttcat cttaaaggcc tggatgatac ccatatcatg
      241 cttggagtat tggatctcat tgacgtcgtc atgattgcga atcttctgat tatggtagtt
      301 atgggcggct atgaaacgtt tgtatcccat ttgcaattgg attcccaccc tgatcaacct
      361 gaatggcttg accattttga tgcaggagct atgaaaatca aattggctct ttcactgata
      421 ggtatctcct cgattcatct cttaagaact tttatagacc ctgcaaaact tagcaattat
      481 tctattatgt ggcaggttgt gattcattta actttaattg tttcagcctt ggccattgca
      541 ttaaccaatc ggttgatgga tcagcccgcc agaaaaaacc tccaacaccc tgattgattt
      601 aaaaatctga aaattaaata acctctttct ctgatgtcac tcactctgca catgacaaaa
      661 cctgttatgt acagagcccc gctctaaaaa aataatgact ggcttaaaat tattcgatag
      721 aatcaggaaa gtttggccta ttcagggaac tcatgggatt gaaccagaaa gaagtagatt
      781 gcaagctctt tcgggttgaa tgtagtcaaa atggaaataa aggaatatga atcatgacag
      841 acactccaaa agctaatcta tccgttgatg aaattaaaga ggataccccc aaagaccagc
      901 ccctccctga agaaaacacg gaaatattgg taaaaaaact gaaagtattg gaaactaccc
      961 aagaaaattt tgatcaaatc aaaaaacata ttgctgcggt tatagattcc ttggcaaaaa
     1021 atcgcagttt ttttggccgt gttgctgcct actggggaga aatgcaatta tggttaaaaa
     1081 ttactcttgg aatagtattg gttgttccca ccttgacact tggaattgca attcaagtgg
     1141 cctctcttat tgtagttagc gtcctgactc tggtgactta tgttggtagt gccctgcttc
     1201 ttgaaaatca tgttcaaaaa gaagagcata ttactgatcg tttaaaagaa gaaatgatag
     1261 gtttggcaga taacctggga aaagttatcg agtcttttga gcctctccgc aagcaaattg
     1321 ctgaacaaat tgagtatttt cataaagaaa atgatagatt aaacctcaac gttactcaac
     1381 tgagtgatca tattatgcga ttaacaaaac aagctgagca attacaaaaa accgaacaaa
     1441 ctttgcgtaa agttagagag gaattggaaa aaacatctga aactctggat gaaaaagtac
     1501 aagttcaaac cgaattacta cagataaatc aggaagaatt aagcagaatc agggaagaaa
     1561 ttgcaaaaaa tgaaattgaa ttatctgaaa aaatttctga cctggatcaa gttaagaaag
     1621 aaatgtcagt agaaattgaa aaagccgaaa ctgttaccca agtattaaaa aatactgtag
     1681 aaaaactgtc ccaaactgct attgcagatc acaatcatag ggaatcgttt caaaataaat
     1741 tgaacgattt tctctctaat aaagaacaaa gtttcaatga tgttgctcaa cgtatatgtg
     1801 aagccgagcg cgaactatgc cttgttaaag aagaattaaa ctttagtaac aaacgctatc
     1861 aggaactatt ggaacgtcaa gaagaagtag ttaaacgttt ggaacaactc caactttccc
     1921 gtaagcctac gccgtatagt aatgcaaaag tacttggtaa attaggtatt atggctactg
     1981 aggaaaaaga gtatccaacg cccgtttcac cagaagaaaa tacaatccca actcaaactc
     2041 tcacaggtta gatactttta aatcgatgtg ctatggcaca tcgattgctt atcacgtgcg
     2101 gttatccaaa aactaatcag tgcaacgaaa tttattttaa aacagatcgc caattatata
     2161 ctgagcataa ataggttcaa actaaagcaa ttcgaagaca gcataattgt tctattaatg
     2221 cgctacttta gatctattgc ccataatcaa tttatattaa atcagttaat ttttttactt
     2281 tatgaacaca aacccacaga gaataataaa agaactatac ttttagaata ttgactgcat
     2341 cagtgaaatg attctttgaa aaggagagag ctatgagcac aaccaaatgg aatttatcta
     2401 tttctcgcgc agttatcttc gtcattgcat tatttgcttc tatgatttgt gctagagctc
     2461 atttcgttgg tggaattcat acaaatgcgg gttatgtggg atggtatcat ccagattatt
     2521 acaatcacga tgttataggc gtgggtaatt atcacccagg ttatggctgg gttgctccag
     2581 tgtatgctac gccaacatat ataataggca atacggggta tgactgccag acagtccagc
     2641 aatgtgattc agagggaaat tgcattcaaa gccaaaactg tgattagtag tggaaaatgt
     2701 agcatatcaa tgcgcactat cttttttaca agattgagaa caaatttcac tccacttagg
     2761 ttctctaatc atcaaaactg ttaattagat taataggtaa acagcagtaa cgataggatt
     2821 ttaaatgttc aaagtaataa gacaaaggct atcaattttt tggttcttta ttcagtaaac
     2881 gattaaatgt atcggtacaa actggtttgt tttttctggt ttgaaaataa ctaagcggag
     2941 taagcagtgc tacattcaca ttcaaagctg gacataaggt tacccattct gcaaagcact
     3001 gctcatccat ttcatcggta ccataccctg tttttgaatt gcccagtttg caataggcaa
     3061 tagaagccat tgccttttca aaggtcgaat tttcatttac ccaaacaggc tcattatcaa
     3121 cgcgaaacaa aaattttgta ccacaatata aaagagccat tcttaagcca gcttggtatc
     3181 tttcattaaa ttcaggttta tagatatcct cctcattaat acatccatct ttttgcttta
     3241 atcgcccttg agcaatatcc tgataaaacc aaatcaatct gcgaatggca atagccgaat
     3301 ccatgtctac tctgttagga gtttcatctc cattcttccc ttccagaaaa tctttgctat
     3361 gactcgctcc attagccaat ttaaaagcaa caaacaacct gtctgcagag agctgagtca
     3421 gtccaaaatt ggtttgataa ttaaatttaa ttccattttg cattgttagt tttaataaat
     3481 tgctccagcg ctgcaaatta gtgccaggtt tgcttgtaga tattgatcgg ccattcatgt
     3541 ctgttacatt agttggatcg ccgctacttt cttttagaat catggcaaga acgtgagcat
     3601 atttcagtac cattttttct ttaaaatagt ggtgctgtgc attcgccata tgctgataaa
     3661 ttacaaaatt ttgaaaaaca agcatcctga gattatagtc tattgcagta caattcttat
     3721 cttcctcttc tgtcattacc tcagcgacat gtctggtaag actttcaact tgctcttcat
     3781 tgggcaagcc aataatatca ggaagccaac tactttctgc cggatcttgt acagctgata
     3841 actgggattg gcctgtaaca aactgccggt tatcttctgc aaagagtggg taggttaacg
     3901 ccgccaataa agataaaaaa acacatgcta ccccattaat acactgtctt tttaatttca
     3961 taaactgcac cttctttatt aagtatagct tgtatccatt ggaagcaggg ctggtataaa
     4021 ttgacataaa acttgaaagt agttcgatta aactaattta ttttttcagc atggcgttga
     4081 gagataagag tatagccaga tacttaacag acaaatcata gaaaattaga acacaaacat
     4141 taatccaaat gcgtcaaaaa tcatacatta ttgaataatg gcaaatgatg tcccaagagc
     4201 aaccaaaata tcgattcacg atatttttca caattttgtc tctcattttc ctctgtacgg
     4261 tacgattttt gggac
//
LOCUS       DAXLDT010000218         4234 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXLDT010000218 DAXLDT010000000
VERSION     DAXLDT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.219"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..225
                     /locus_tag="ACF2SP_002879"
     CDS             <1..225
                     /locus_tag="ACF2SP_002879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360643.1"
                     /translation="HPYITIFIAFFIAIFIGGGMASEEYAKKLRNKEEELNKYHGIEN
                     EIEEIEFALDDKDIESAKDSLETLKSRLNL"
     gene            554..1399
                     /locus_tag="ACF2SP_002880"
     CDS             554..1399
                     /locus_tag="ACF2SP_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360644.1"
                     /translation="MSQNLDQSLSDMSEEEQISYMEDWFRDNYEDPAQECPYAEGEYI
                     YIYGGPYDAYEELEGQFSDEVPERLITQLADKLSLECYNWSGKEVPSKIFDYIEPNPE
                     YYEDFRNNLNMIEKIADLQFDEDVRLHTLKLLYINVITALETYLSEAFTTLINSNPIY
                     KRQFLEKNKDFNERKLPISNIYKQYEAIDDYIKQYLSDLLWHRLEKIEKLYLAAFNLH
                     FSENKQPILYAIHMRHDLVHRGGKDKDGNILVITKNDVIDLTKSVRLFGIHLYSQLTS
                     LNQSN"
     gene            complement(1697..2062)
                     /locus_tag="ACF2SP_002881"
     CDS             complement(1697..2062)
                     /locus_tag="ACF2SP_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HFD2360645.1"
                     /translation="MKKSLMENILTCRLGQQRTNPRKYKVEFLALREDISEALEKGWS
                     ITIIWETLRDEGRFTATYNTFRLYVLKYLNGQKFRYSQKEANDQQPKGLAKNQDKEKK
                     PTAIPSFTFNPIPNLKELL"
     gene            complement(2496..2726)
                     /locus_tag="ACF2SP_002882"
                     /pseudo
     CDS             complement(2496..2726)
                     /locus_tag="ACF2SP_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2820..3059
                     /locus_tag="ACF2SP_002883"
     CDS             2820..3059
                     /locus_tag="ACF2SP_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360646.1"
                     /translation="MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENS
                     PDNSRIDTLFRILSALNLNMSLVEKDQKTHEDIEW"
     gene            3059..>4234
                     /locus_tag="ACF2SP_002884"
     CDS             3059..>4234
                     /locus_tag="ACF2SP_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFD2360647.1"
                     /translation="MRKLYILMNNELVGVLTQDNAGGLSFSYVADWISKPHSRPVSLS
                     LPLINQRFSGEVVYNFFDNLLPDNPQIRARIQARFQAKSSRPFDLLALIGGDCVGAIQ
                     IVERIPQTQRLSIESHVVSEAEIARTLRSYQTSPLGMDKEDDEFRISIAGAQEKTAFL
                     YYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLEVAKSK
                     ILQFEDEKVLVVERFDRKWSRDNRYILRLPQEDLCQALGYSPNLKYQSDGGPGIKEIM
                     QLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQPQGGYQLTPLYDIISAY
                     AIVEQKQLSFKKLKMAMALYGKNSHYKIDGIQKRHFIETAKYVQYSKIKAAQIFEQVI
                     "
BASE COUNT         1379 a          761 c          792 g         1302 t
ORIGIN      
        1 catccctata taactatttt tattgccttt tttattgcca tttttattgg aggtggtatg
       61 gcaagtgagg aatatgcaaa gaaactaagg aataaggaag aagagcttaa taaataccat
      121 gggattgaaa atgagattga agaaattgaa tttgctctag atgacaaaga tatcgaaagt
      181 gcaaaagatt cactggaaac tttaaaaagc cgacttaacc tataaattgt ttagcgagca
      241 tgaataatgg ggtcaggccc agaattgtga attttcaatt gaatatcacg tctatgactc
      301 ctattcaaat ttcaagatct gctcccatta ccacttccag aaattaacta aagtagttga
      361 taagggttat ttaatacaac taatgaagat cgggtgcaga caacttgtgt gcgaatgatt
      421 ggaattgaca atagtctaaa gggctggctt ttcttttaat gactagagga gagatctata
      481 agactattac caaaaccaaa tatccagcct ctgctatatt tttcataatg ggagcatgaa
      541 ataggttaat aatatgagtc aaaatttaga ccaatcctta tcagatatgt cagaagaaga
      601 acaaatatcg tacatggaag actggtttcg tgataattat gaagatcctg ctcaagaatg
      661 tccctatgca gagggcgagt atatctatat ctatggtggg ccttatgacg cttatgaaga
      721 acttgagggg cagttttcag acgaagtacc agagagactt attactcaac tagctgataa
      781 gctgtcgttg gagtgttata attggtcagg aaaagaagtt ccttcaaaga ttttcgatta
      841 tatagaacct aaccctgaat actatgagga tttcagaaat aacttaaata tgatagaaaa
      901 aatcgcggat ttgcaatttg acgaagatgt tcgattgcat actttaaaac tactatatat
      961 aaatgttatc actgccctag aaacttacct ttcagaggct tttacaacac tcattaattc
     1021 taatccaata tataaaagac agtttctaga aaaaaataaa gattttaatg agcgtaaact
     1081 ccctatatca aatatatata agcaatatga agcaatagat gattatataa aacaatactt
     1141 atctgacctt ctttggcaca gattagaaaa aattgaaaag ctctatttgg ctgcatttaa
     1201 tttacacttt tcagaaaata agcagcctat attatatgca atacatatga ggcacgatct
     1261 tgtacatcga ggcggaaaag ataaagatgg gaatatatta gttataacta aaaacgatgt
     1321 cattgacttg acaaaaagtg tgcgtttatt tggcatccat ctctattcac aattaacaag
     1381 tctcaatcaa tccaattgac caaactgttt tgttgaatta aagagacgcc aaatgaaatg
     1441 agaacaaata aaaactcatt aattcgaaaa gctaaaagga tgtaaagtga aactcccagg
     1501 ggtagactcg actgattaaa attttatctt cacgaaaata catacacaca aacatgttct
     1561 ccaatgtctc agggacaaaa gggacatttg gggcaggcac gtcactacta ggttttagcc
     1621 gtccccaccc tgtatcatca agatggggta tgtgggactt gttgaaatct atatcgacca
     1681 ataaaaatgc attccatcat aatagttctt ttaaatttgg tataggattg aaggtgaacg
     1741 aaggaatcgc ggttggtttt ttctctttgt cctgattttt cgccagtccc ttcggttgct
     1801 gatcgttagc ctccttttgt gaatacctga acttttgacc attcaagtat ttcaatacgt
     1861 ataacctaaa cgtattgtaa gttgctgtga agcgaccctc atccctaagc gtttcccata
     1921 tgattgtaat agaccatcct ttttccaagg cttcggatat atcttcgcgt aatgcaagaa
     1981 actccacttt atattttctg gggttcgttc tttgttgacc cagcctacaa gtcagtatgt
     2041 tttccattaa ggattttttc atagtctaat ctccatatta ctggttatcc aatcctttga
     2101 gtttttagtt tctccctacc cacgtcccgc tgcttgtcag cgggatccac tcgatctaaa
     2161 ttaagaactc tggaccccgc tgacaagcag cggggcgtag gggtgaatag ttacgtttat
     2221 ttttatttat tttcgacctt ttaggccaca tgcaacgccc tttttggtaa ctttcggtaa
     2281 tttattattt atttttgttt attcatgatt attttcgtta ttaactgctc tataacgaac
     2341 aattgcgcat aattacaaaa gtatggttcc taaataaatt tcaaaatgca gggcaagata
     2401 tgtgaagtta gctaaccagc aagctggtta ccaaacttca cgagtcttat tctcaccttc
     2461 ggtgctcaac ggaaaagcca ttttcatagt caattttatt cacctcccca aattttaccg
     2521 gtaaacacat acaatcttgg ggtgccaact cctttgattc ttggcttatg tgaggcttta
     2581 ccatcagggg cgggtttcac ccagccagct tgtaataaaa ctcttgtcac tgcgcgttga
     2641 tcgaagcctt tgcaaagttc atttttgtac gtctcagtta acaccatgta gattcgaaat
     2701 ccttggctat ctgtatgata aaatcctgtt ttaccctcaa atgcgggtac aattgatttt
     2761 acccgcactt gagggtatag ttaatattat tggcgattgg gggtaaaaag aggaaatcga
     2821 tgatcattag ctcagcaaaa gatttagcat tatatttaaa tgacaagcgc aaacaactca
     2881 aaatgagtca atctgaagtt gccgatttag tcggcttaaa acaggatacc atatcaaagt
     2941 ttgaaaatag ccctgataat agtcgaattg ataccttatt tagaatattg tccgccctaa
     3001 atttaaatat gagccttgtt gaaaaagatc aaaaaactca tgaagatata gaatggtaat
     3061 gcgtaaatta tacatattga tgaacaatga gctcgtgggg gtgcttaccc aggataatgc
     3121 aggtggtttg agtttttcct acgttgctga ctggatttcc aagccgcatt cacgcccagt
     3181 ttctttatcg ttaccgttaa taaatcaaag gttttcagga gaagttgttt ataacttttt
     3241 tgataattta cttcccgata atccacagat aagagcgaga attcaagctc gttttcaggc
     3301 aaaatcatcg cgaccgtttg atttattggc gttaattggc ggtgattgtg ttggtgcaat
     3361 acaaattgtt gaacgtattc ctcagacaca gcgtctaagc attgaaagtc acgttgtcag
     3421 tgaggccgag atagctcgta ctttacgttc ttaccaaacc tcaccactgg gaatggataa
     3481 agaggatgat gagtttagaa tctcaatagc cggtgctcag gaaaaaacgg cttttctata
     3541 ttaccaaaat gaatggcata aaccatttgg acctacacca acaactcaca tattcaaatt
     3601 acctattgga tatttaccac atcaaggaat tgatttaagt gagagttgtg aaaatgagca
     3661 tctctgttta ctactagcca gcaaatttgg tttagaggtt gcaaaatcaa aaatcttaca
     3721 attcgaagat gaaaaggtgc tggttgtcga aagatttgat agaaaatgga gtcgagataa
     3781 ccgttatata ctcaggttac cgcaagaaga cctatgccaa gcgttgggtt attcaccaaa
     3841 tttaaaatat cagtcagatg gtggccccgg tattaaagaa attatgcagc tacttaatgg
     3901 ctcacagcag cctcaaaaag atagagatga atttttcaga gcacaggttg tttactggtg
     3961 ccttggtgct attgatgcgc atgccaaaaa cttcagtata agtatacaac cacaaggcgg
     4021 ttaccagctc acccccttat acgacatcat ttcagcctat gctatcgtgg aacaaaaaca
     4081 gctatccttt aagaaattga aaatggcaat ggctttatat ggtaaaaata gtcattacaa
     4141 gattgacggt attcaaaaaa gacactttat tgaaacagca aaatacgttc aatattctaa
     4201 aattaaagca gctcagattt ttgaacaagt gatt
//
LOCUS       DAXLDT010000219         4213 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXLDT010000219 DAXLDT010000000
VERSION     DAXLDT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.220"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..983)
                     /locus_tag="ACF2SP_002885"
     CDS             complement(<1..983)
                     /locus_tag="ACF2SP_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360648.1"
                     /translation="MVKKVKKFDMPQAQLWTILNAQQNKKQQTNSPSKEIKKFDMSEA
                     QLWAILNAQQNKKQQISSPSDEKGDSYIKFFNKHSREHLKKEYDPNGNANQPSKIIKD
                     AQSSIGALSRIFDEYDCPTPNYEEINAIYQKPNGLREHIQENLKGIIPVLLNEDRTAL
                     HPDVIEAIGQENYNAILNNAQGDERAIAEQFVKAVISGYGQRIYQNTDDGYDLHLMSE
                     LPESTEQYKNSYLFIKKGDTRELYYIKPDGKYEKVEINDFNLFEETINAIKNKDETKL
                     HLSEKQIKEIVTSNGSHTPENLKFNLFNTVMPALGELANEVTLKGLPEQSK"
     gene            1489..3441
                     /locus_tag="ACF2SP_002886"
     CDS             1489..3441
                     /locus_tag="ACF2SP_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFD2360649.1"
                     /translation="MPHSTNQSNVIENINQYLKWHNLPVRFGTGGVCNGLSTVHAQYV
                     LQGREREFFELLRYVAGDKGILDANDSVKEKVNDFVWKVVASHMTSGHDKELNQLNSF
                     KTLSINNKPLKSVFDLPLVTSDKNWENILESLNLKEDEVMLVRSINHAISITRKGNQY
                     HVYDPNYEEGVKSFSSEQEVIKELHERVFHYNKGNMGLTLSIITTGDKEPRQDLPKPV
                     DIYDQYLNKENVNSLATITVEHPLGGPWDLTKKTIDLASECGDENLINKLIELHQFDF
                     NNMPIEQASQAVQYNNVRAAELLVNDMIRILSKQDPVAQDEFSDIPAEELPAEFLKQF
                     EEIPDAPEGDEIKEAEVQNPKVVMPPSEKKIKDLLYPLILKAASYGRKEVFDTLCEMW
                     NKNAGDFDKEDLKNFILLAAKGGNSVLLQELIDKYYESNMTLYDKDIQGKVDGIIHEL
                     ISKGSPDSAVCIEVLLNTLSKEGKSIDDRQRADYISRAFEANKPHMIERFIKGISDEK
                     RLEFLIRAIKKDQPHMVELFIKDLPKGLLNTISLSLPAIEKMNGETLKILEKNGMKFT
                     EMEKAIIAKKSHQPVGLLLSIGVALAKFTDFCKEVLFKNESVTYDKNKFQAFKSQLNS
                     IKTQNSLANNEPVEAQQNDQVAVYRP"
     gene            3601..3744
                     /locus_tag="ACF2SP_002887"
                     /pseudo
     CDS             3601..3744
                     /locus_tag="ACF2SP_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144722.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(3804..4103)
                     /locus_tag="ACF2SP_002888"
     CDS             complement(3804..4103)
                     /locus_tag="ACF2SP_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFD2360650.1"
                     /translation="MSFTEPRVLPMKQSIGAGLTAFGLIANRKADSIGSGFSLAWLNQ
                     RVTNRKTELMFQIYYQAKLFNFIYLEPVLSYIPTPGQSSHLNPASAGTLRAIVLL"
BASE COUNT         1385 a          660 c          808 g         1360 t
ORIGIN      
        1 tctttagatt gttcaggcaa acctttcaaa gttacttcat tggcgagttc tcccagagcg
       61 ggcataactg tattaaataa gttaaatttc agattttcgg gggtgtgact tccgtttgat
      121 gttacaattt ctttaatttg cttctcgctg agatgtaatt tagtctcatc cttattttta
      181 atggcgttta ttgtttcttc aaacaaattg aaatcattaa tttctacttt ttcatatttc
      241 ccatcaggct taatataata cagctcccga gtatcgcctt ttttgataaa tagataactg
      301 tttttgtatt gttcagtact ctcaggtaat tcggacatca aatggaggtc atacccgtcg
      361 tcggtatttt gatatatcct ttgcccatag cccgatatta cagccttgac gaattgctca
      421 gcaatcgccc gttcatcacc ctgagcatta tttaaaattg cgttataatt ttcttgccct
      481 atcgcttcaa taacgtctgg atgaagtgcg gttctatctt cattaagtaa aacagggatt
      541 atccccttta gattttcctg aatatgctct cttaatccat taggtttttg ataaatggcg
      601 tttatctcct catagtttgg agtcgggcaa tcatactcat caaatattcg tgacaatgct
      661 cctatcgaac tttgagcgtc ctttattatt ttactgggtt gattggcatt accgttgggg
      721 tcatattctt ttttcagatg ttcgcgtgaa tgcttattaa aaaacttaat ataagaatca
      781 cctttttcat cagatggact acttatctgt tgttttttat tctgttgagc attaagaata
      841 gcccataatt gagcttctga catatcaaat ttttttatct ctttagatgg actatttgtc
      901 tgttgttttt tattctgttg agcattaaga atagtccaca attgagcttg tggcatatca
      961 aattttttta ccttttttac catgataatt tctccaaaat ttaaattgta taaaattagt
     1021 acaattacag tttaattacc actcattaag gttaagttat atccatatca aataattgaa
     1081 taaggcatca ataaagctga taagtaatga accatggtat tgaccatgaa cgaggttctg
     1141 tatcgcatat tcataacaat gctgaatgcg tttaatgagt atttgaaggc taagtttttg
     1201 atagggcttt ttaaaacgat cgaaaggact taaacccaat tcgttttgct tgaggataac
     1261 accaaaatgc tacctgaata tctgctttgt ttactcagag ttcttgggta tggtctgaat
     1321 atcgctttgc gcaatacctc ctcgccaaca atccaactgc gaaagggtat tatcatgctg
     1381 cgatatcaat gttatgcatg ggggataggg gatattaata cttacaaaat gtttaattaa
     1441 taatcattct gtaatatgga agcataatac tctaaatagg agaatattat gccacatagt
     1501 actaaccaat ctaatgttat cgaaaacatc aatcaatacc tgaaatggca taatttgcca
     1561 gtaagatttg gtactggcgg agtttgtaat ggattgtcta ctgttcatgc ccaatacgtt
     1621 ttgcaaggta gagaaagaga gtttttcgaa ctgttacgat atgttgcggg ggataaaggt
     1681 attctggacg cgaatgattc cgtgaaagag aaggttaatg attttgtttg gaaggttgtt
     1741 gccagtcata tgacgtcagg gcatgataaa gaactcaatc aattgaattc ctttaagaca
     1801 ctgtctatta ataataaacc attaaaatct gtatttgatt tacctcttgt taccagtgat
     1861 aaaaattggg aaaacattct tgaaagcctt aatttaaaag aagatgaggt gatgttagta
     1921 cgtagtatta atcatgcaat cagtattact cgcaaaggca atcaatacca tgtttatgat
     1981 cccaattatg aagagggagt gaaatcattt tcctctgaac aggaagtgat taaagagctg
     2041 catgaaagag tatttcatta taataaagga aatatggggt taacactatc cataatcact
     2101 acaggggata aagagccgag acaggacctg ccaaaacctg ttgatattta tgatcaatat
     2161 ttgaataaag agaatgttaa tagtttagct accatcacgg ttgagcatcc attgggagga
     2221 ccatgggatt taactaaaaa aactatagat ttggcatctg aatgtggtga tgaaaatctg
     2281 attaataaat taattgaatt acatcagttc gattttaata atatgcctat tgagcaagca
     2341 tcccaagcgg tgcaatacaa taatgtaaga gcagctgaat tattagtaaa tgacatgata
     2401 agaatattgt cgaaacaaga tccagttgca caagatgaat tttctgatat ccccgctgag
     2461 gaattaccag cagaattctt aaagcagttt gaagaaatac ccgatgcgcc agagggagat
     2521 gagattaaag aagcagaagt gcaaaatcca aaagtagtga tgcccccaag tgaaaaaaaa
     2581 attaaggatt tgttgtaccc tcttattcta aaggctgctt cttatggaag gaaagaagta
     2641 tttgatacgc tatgcgagat gtggaataag aatgcaggtg attttgataa agaagattta
     2701 aaaaatttta ttctattggc agcaaaagga ggtaatagtg tacttttaca agagcttatc
     2761 gataaatact atgaaagcaa tatgacttta tatgataagg atatccaagg taaagtagat
     2821 ggcataattc atgaattaat ttcaaagggt tctcctgata gtgcggtttg tatagaggtg
     2881 ctgcttaata cattatcgaa ggagggtaaa tcaatagatg atagacaacg cgcagattac
     2941 atatcgcgtg cattcgaagc aaataaacca catatgatag agcgcttcat caagggaata
     3001 agtgatgaaa aacggttaga atttttaata cgcgcaatta aaaaagatca gccgcatatg
     3061 gttgaactct tcattaagga tttaccaaaa ggacttttaa ataccatatc gttgagttta
     3121 cctgcaatag agaaaatgaa tggggagacg ttaaaaatac tggaaaagaa tggtatgaaa
     3181 tttactgaga tggaaaaagc cataattgct aaaaagagcc atcaaccagt tggtttgtta
     3241 ctgtcaattg gtgttgcctt ggcaaaattt acagatttct gtaaagaggt tttatttaaa
     3301 aacgagagtg tgacttatga taaaaataaa tttcaggcat ttaagtctca attaaatagt
     3361 ataaagaccc aaaatagttt agcgaataat gagcctgttg aggcacagca aaatgatcag
     3421 gttgctgtgt atagaccttg attggcacgc tcggagagac tagaactcca agtactttag
     3481 tgcgaagctg atggtgtgat aatttcttat aaatcaatat attgtgaacc gtcaaattca
     3541 cctgaaatag cctattaccg cgaggtgtcg ctattatgag gtctgttatc aactggctaa
     3601 aaaaatattt ccaatattga acattcaaag catcgctcta tattcaattt tatggtcaat
     3661 atattggccg gtttagctgc ttatgcactt aaaccaaaga agctctcttt aaatattaaa
     3721 cgatctttta tggctgttgt ttgagcagtt atatcgaatt catggtagat aggttaatca
     3781 aagagaacac acaatttgac ggtttaaagt aacactatgg ctcgtaaggt cccagctgat
     3841 gctggattga gatgactact ttgccctgga gtagggatat aagagagtac tggctcaaga
     3901 taaataaaat taaataattt tgcctggtaa taaatctgaa acattagctc tgtttttcta
     3961 ttggtaactc gctgatttag ccaagccaaa gaaaacccgg atcctattga gtctgctttt
     4021 ctattagcaa ttaatccgaa tgcagttaaa cctgcgccaa tagattgttt cataggtaat
     4081 actctaggtt ctgtgaacga aatttttaat taattgaaaa gaaggcagtt ttatagtgga
     4141 attcgattga cgatcgtaaa aaacactaat ggaactgcca gaatgcatca taacatatca
     4201 gaagaattgt gga
//
LOCUS       DAXLDT010000220         4190 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXLDT010000220 DAXLDT010000000
VERSION     DAXLDT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.221"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(227..2992)
                     /gene="ankF"
                     /locus_tag="ACF2SP_002889"
     CDS             complement(227..2992)
                     /gene="ankF"
                     /locus_tag="ACF2SP_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkF/LegA14/Ceg31"
                     /protein_id="HFD2360651.1"
                     /translation="MKDQLAKIFEKYPFVHLDKSGELSIIVPLTGAASISDNFTADET
                     KPIFLYLFGDNTCKSDLERRNFFYGKQKSGGRHSVKPFTELFSNLIHELNGLKSSTEI
                     RVDEQVFLETIIANLNELKEQALAFRKGNPGFEDMKNDFSHSDEIMVSKSEFVVTVYP
                     DRVRQFDLPNVMSLINPGESRYKQLLAQSNINQCLDALSLVQKPAAEGNLDLIADALG
                     KLGADPIDYLCKKDNKDPKALFKAVYEILQKQDTSLENQQSAVFEVMESNLVAAFGDN
                     EDFSTWQKEEIVDAIKSIFKQANLLSEQSGTKQKTFADVYNQAFDKYNESTNDDSESG
                     CKRRNTYQLFILQTFLYLCSLQLRLKNKEKAKKFLGFFEDKGTLNNLVLQLCTDEQAF
                     MRMMADEFELSSAEYASIAESTYGIVLAHIDAEHFDELRIACSAQLLQDSNYIVMGGR
                     LCWSTSSINEEGNIRSLEQQKKISSENFEIFYNQRQSYIDKSREFQQIKVLLDKDVND
                     EGIAEAVASKVRYLTKYQKSQILSEVIQKKKYEHAKLLIENYANLVFPSVFISVIKST
                     PVHIDLIRAFLNADSRHALNIDGNDLIQAAKKGDFELVKIITEHRPDLLESKDSFQQT
                     PLLWACANGYVDIVEYLMDSGADIHVRTKVSGSHRYKIGETPTGGRTALDWARCYDKT
                     DPVKASKIIALFDTYYQRMEDHFKANIAYKDQFNKAHLTQAINNGDLKLIALFLKYCP
                     HLEKFFTQEEQLRAKQRHNQQMLRSQIMPYQNSFNKLLDELQRKAELLNKKYGTLHEA
                     TNVMQALINDLSTVGNDFFMDDITPQSFQKFERKCKQVLEEASPVLSTHRGWHGYPDF
                     VRRIIGVIATLAVIPALVVHFSSAKGYVGVFFSGKENIKTDSSEKMGEFANNLETLKT
                     EISNKLS"
     gene            3216..3710
                     /locus_tag="ACF2SP_002890"
     CDS             3216..3710
                     /locus_tag="ACF2SP_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360652.1"
                     /translation="MAFQRSFFSHIEDDWRYYQNIRAKYSDAIPIPQRKYFEPIHSID
                     SFATLAVRSIEKPLWLGVHTAGFLLKAIIHLVGALVLSPFALIFAICVPRSELREQTV
                     SSFKSTAAGSIVAAGMACVALLSTLMSLIFNPLYALSRSAATGIDHLNSVTESCCGLT
                     IAKI"
     gene            complement(3758..>4190)
                     /locus_tag="ACF2SP_002891"
     CDS             complement(3758..>4190)
                     /locus_tag="ACF2SP_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360653.1"
                     /translation="LKTFNEFLLEQGGTTYRAVDHYLKGKDTPLKSVFFAPYSSAPNF
                     LYRAGHVITAPISFSIITLELVSSSLYLSLKSLNSLVFSDKKAAKIHIIDSVVHFAVS
                     LITAIGVIVSPIINLIDLIGGAISTMRVKSEPAEQMRPSVL"
BASE COUNT         1260 a          817 c          716 g         1397 t
ORIGIN      
        1 aaatcagata ataattgaaa acctaatttt aatttttgga gatattcctt taagtcgatg
       61 ggtttcatag aaaactaaat catattactt ccttgcatca tatctggttt aagtttgaaa
      121 ataaagtgtt taattagaac aaaaatcaag ggctccatag aaccctttgt ctgcaaccca
      181 aacagaatag agcgaaacat ttagcttcag cagctaaatt ctccagctaa gacaacttat
      241 tggatatttc tgtttttagg gtttccaggt tattcgcaaa ttcacccatt ttttcagaag
      301 aatctgtttt tatattttct ttgccagaaa agaaaactcc tacatatccc tttgcggatg
      361 aaaagtgtac aaccaatgca ggaataactg ccaaggttgc aataactcct ataattcttc
      421 tgacaaaatc aggataacca tgccaaccac gatgcgttga taaaactggg ctggcctcct
      481 caagaacctg tttacacttc ctttcaaatt tttgaaagga ttgaggcgtg atatcatcca
      541 tgaaaaaatc atttcctact gtggataagt cattaattag tgcttgcatg acattggttg
      601 cttcgtgaag agtaccatat tttttattta gtaattctgc ttttctttgt aattcatcta
      661 ataatttatt gaatgaattt tgatacggca taatttgaga acgcaacatc tgttgattat
      721 gtctttgttt tgcacgtagt tgctcttctt gagtaaaaaa tttttcgagg tgtgggcaat
      781 actttaaaaa caatgcgatt aattttaaat ccccattatt tatcgcctgg gttaaatgag
      841 ccttgttgaa ttggtcttta taagcaatgt tggctttaaa atggtcttcc atgcgctgat
      901 aataagtatc aaataaggca attatctttg atgcctttac aggatcggtt ttatcatagc
      961 atcgcgccca atcaagtgcg gttcgtcccc cagttggggt ttctccaatt ttatatcgat
     1021 gactcccgga cacttttgtt ctgacatgaa tatcagcacc tgaatccatt aaatattcca
     1081 cgatatccac atagccattg gcacaagccc aaagcaacgg agtttgttgg aaagagtctt
     1141 tggattccaa taaatccggt ctatgttctg taatgatttt aaccagctca aaatctcctt
     1201 tttttgctgc ctgaattaaa tcattcccgt caatattcaa cgcatgacga gagtctgcat
     1261 tgagaaacgc tcgaatcaaa tcaatatgaa ccggtgtact ctttatcact gaaataaaca
     1321 cggatgggaa aacaagattg gcatagtttt caattaaaag ttttgcatgt tcgtatttct
     1381 ttttttgaat cacttcgctt aagatttgtg acttttgata tttcgtcagg tagcgaactt
     1441 ttgacgccac agcttcagct atcccttcgt cattcacatc tttatctagc agaactttta
     1501 tctgttgaaa ctctcttgat ttatcaatat aagattgtct ttgattataa aaaatttcaa
     1561 aattttcaga tgaaattttc ttttgctgct ccaaacttcg tatatttccc tcttcattaa
     1621 tagaactggt gctccaacaa agacgtcctc ccatcacgat ataattggaa tcctggagca
     1681 attgagcact gcatgctatt cttagttcat caaaatgttc agcatcaata tgagccaaga
     1741 cgatgccata agttgattca gcaatagacg cgtattctgc agaagacaac tcaaattcat
     1801 cggccatcat tctcataaac gcttgttcat cagtacataa ttgtaatacc aaattattca
     1861 acgtcccctt gtcttcaaaa aaacccagga atttttttgc cttttctttg ttttttaaac
     1921 gcaactgcaa agaacataaa tagagaaacg tttgtaaaat aaacaactga taagtgttcc
     1981 tgcgtttaca tcctgattca gaatcatcat tagtgctttc gttatattta tcaaaagcct
     2041 gattgtagac atctgcaaat gttttttgct tggttcctga ttgttcactc agcaaattcg
     2101 cttgtttaaa tattgattta atggcatcga ctatctcttc cttttgccag gtactaaagt
     2161 cttcgttatc tccaaaggca gcgaccaaat tgctttccat gacttcgaaa acagctgatt
     2221 gttgattttc taaagaggta tcctgttttt gcagaatttc ataaaccgct ttaaataaag
     2281 ctttaggatc tttattatct tttttgcata agtaatcgat ggggtctgca cccagttttc
     2341 ccagcgcatc agcaatcagg tccaagttac cctctgcagc aggcttttgc acaagagaca
     2401 atgcatccag gcattgatta atgttggact gggcaagtaa ttgcttgtag cgggattccc
     2461 cggggtttat aagactcatt acgtttggca aatcaaattg tcggactctg tcaggataca
     2521 ccgttacaac aaactcgctc tttgatacca tgatttcatc gctgtgacta aaatcatttt
     2581 tcatgtcttc aaagcctgga tttccttttc tgaaagcgag agcttgctct ttcaactcat
     2641 taaggtttgc tatgattgtt tcaagaaata cttgctcatc cacacggatt tcagtagacg
     2701 atttgagacc atttaattca tgaatcagat ttgagaacaa ctcggtaaag ggttttacag
     2761 agtgacgacc tccggatttc tgcttaccat aaaaaaaatt tcttctttcc agatctgatt
     2821 tgcacgtatt atcaccaaat aaataaagaa aaattggctt ggtttcatcg gcagtaaagt
     2881 tgtcggaaat actagccgcg cctgttaaag gcacaattat tgatagctca cctgatttat
     2941 ctaaatgaac aaaggggtat ttttcgaata ttttggctaa ttggtctttc aatttcgtta
     3001 tactctttca tgttaagctt ttttggcacc attgtaatat agtttgctta agacaaaatt
     3061 aataacactg tggtcataaa taagcccagt aaaaacctct tgttgtctct gtcaacaaga
     3121 taatcccaag atttgaaaaa tgaagaactt gcctattatt tacaaacact gccaattttt
     3181 ttaatatccg ctgattcaat aacaaggagt ttccagtggc ttttcaaaga tcattttttt
     3241 ctcatatcga agacgattgg cgttattatc aaaacattcg tgcaaaatac tctgatgcta
     3301 tacccatacc acaaagaaaa tatttcgaac ccattcattc catagattca ttcgccacat
     3361 tagcagtacg gtctattgaa aaacctcttt ggcttggtgt tcacacggca ggttttttgc
     3421 tgaaagcgat aatccactta gtaggggcat tagttctttc accatttgct ttgatctttg
     3481 cgatatgtgt tccgagatct gaattgagag agcaaactgt ctcatcattt aaatctactg
     3541 ctgccggatc tattgttgct gcaggtatgg cttgtgttgc attactctca actttaatgt
     3601 cacttatttt taatccgttg tatgcattaa gtcgaagtgc cgccacagga atcgatcatc
     3661 ttaattcagt aaccgaatcc tgttgcggtc tgactatagc aaaaatctga ttaggtggtt
     3721 caatcgcaac ccttatttat ttcaaatctg gcgaaaacta gagaacagat ggtctcatct
     3781 gttctgctgg ctcacttttt accctcatag ttgatattgc tcctccaatc aaatcaatca
     3841 aattaataat ggggctaaca atcacaccaa ttgcggtaat aagagaaaca gcgaaatgca
     3901 ccacgctatc aataatatga atctttgcgg cttttttatc agaaaaaacc aggctgttga
     3961 gagactttaa gctcaaatag agggacgatg aaacaagttc taaagttata atagaaaaac
     4021 taattggtgc cgttatcaca tgacctgctc tgtataaaaa attaggggca gatgaataag
     4081 gagcaaaaaa aaccgatttg aggggggtgt ccttcccttt caaataatgg tcaacagccc
     4141 ggtaagtagt accgccttgc tctaataaaa attcattgaa tgtttttaag
//
LOCUS       DAXLDT010000221         4183 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXLDT010000221 DAXLDT010000000
VERSION     DAXLDT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.222"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            46..126
                     /locus_tag="ACF2SP_002892"
     CDS             46..126
                     /locus_tag="ACF2SP_002892"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360654.1"
                     /translation="MYAENQISLDGLYQDLSQHPAVKMVL"
     gene            126..725
                     /gene="lipB"
                     /locus_tag="ACF2SP_002893"
     CDS             126..725
                     /gene="lipB"
                     /locus_tag="ACF2SP_002893"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFD2360655.1"
                     /translation="MIIHNLGIKDYTEIWEQMKEFTATRDSNSCDELWLLEHYPVYTQ
                     GQAGKPEHVLNPNSIKIVQSDRGGQVTYHGPGQLVAYVLMDIRRRNLGIRTLVVKLEE
                     ILISVLEHYRIPANIRSGAPGVYVGEKKVASIGLRVKNGCTYHGIALNVNMDLSPFLG
                     INPCGFAKMEMTQMSHFHPNIQLEDVSQHFVQYFLTQFK"
     gene            735..2771
                     /locus_tag="ACF2SP_002894"
     CDS             735..2771
                     /locus_tag="ACF2SP_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFD2360656.1"
                     /translation="MNLLADYVQPLTYWLQANPHWSLFITFLIALSESLAVIGSIIPG
                     SVTMTAIGILAGSGIMRIDLTLLAATLGAICGDSLSYFLGYFYSDRLIEIWPFRKYPG
                     LLKYGKDFFASHGGKSVLIGRFVGPLRSIIPVIAGIMHMKQWRFLIANIVSAIGWSIL
                     YVMPGVLIGAAGHELSAEGATRFFILILVILAVIWLSSLVLKWLFIKLNSFLRSNLHR
                     FWLEIKTSPRLSKVYYAITPVQEENHYNTVGLLLIALVIFFLFLTLVIFTIEGQLLDY
                     INVPVHFFMQSFHSSLLEAVFIVCAQLTSKSTLTGLFVASCIWFIYHKNYRATVYLSS
                     ITLLSGVIALFLTKIIDSPRPQGLLVTLPGSSFPSVSISIATALYGFVLFYINSKYSL
                     ITSSFKTIILIVLGLSGFANIYLGDFWFSDILAAYLLGAEVCLIHCLVYRRFSTSVIK
                     NEHPLIMLISLLMVIVFATAVSTVVNFKEQVHDHMPYHKEFTLTEKQWWDQQKPLLPL
                     YRLSRIGKRISLLNIQYVGDLDVLQNKLEQFGWEPQSDKTFFTRLLMRMNNAPSNVKL
                     PLLTQLYENKKPGLIMTYQDKKAKLIFDLTIWQSNYYLSEPKRIVWIGSIHQNIFNLK
                     QKKPKHNLPPVLNPLSYVIPALDDFTLRRIVLSDDLVKTSVFPTAPFILLIKTK"
     gene            2898..3503
                     /locus_tag="ACF2SP_002895"
     CDS             2898..3503
                     /locus_tag="ACF2SP_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system protein LssZ"
                     /protein_id="HFD2360657.1"
                     /translation="MHILAKVIHCIFPLIALTLLIIGIKRNAIYYIISSLWISLISLV
                     IHYQTSGGEILGTYFNYLNATIYTINLLVLCLSLVCVLWHLSNDNVAFKIVSSLFQSF
                     IVIGALLVVINLWINAYFIESRLIGTPVMQVALLEKPEYCDYRYIFYKVALDGTVHYL
                     CPNHYGLIPSIGRLSISPDFIASQLSIPSKKQMLLLQQKRS"
     gene            3588..>4183
                     /locus_tag="ACF2SP_002896"
     CDS             3588..>4183
                     /locus_tag="ACF2SP_002896"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HFD2360658.1"
                     /translation="MKRFFSILFILYCCNLFAMAPKLNWDKAIDKAIKRYGLRVEPQL
                     ISYFSKAGVSYPPKEIALLAFKSERKVELWAKNPSQEWTHIHDYPLTGFSGRLGPKLR
                     ENDKQIPEGIYKLVNFNPFSSMHLSMMINYPNSFDRQKGYMDGRKELGNNIFIHGKNL
                     SVGCLAVGDLAIDQLFILARRVGLKNIQVIIAPNDLRY"
BASE COUNT         1262 a          652 c          793 g         1476 t
ORIGIN      
        1 cataaaacga gtgaacaaaa caagtacttg gctattactg tgacggtgta tgcagaaaat
       61 caaatctcgc tggatggatt atatcaggat ttaagtcaac atcctgctgt gaaaatggtt
      121 ttataatgat aattcataat ttaggcataa aagattatac tgaaatatgg gagcagatga
      181 aggagttcac tgcaacccga gacagtaatt cttgtgatga gctttggtta ttagagcatt
      241 atcctgtata tactcagggg caggcaggga aaccagagca tgtacttaat cccaactcta
      301 taaaaattgt gcaatcagat cgtggcggac aagttacata tcatggccca ggtcaactag
      361 tagcctatgt tttaatggat attcgccgca gaaacctggg cataagaact ttagtagtaa
      421 aactggaaga aatattaatt tcggttttag aacattatag aattccagca aatattcgca
      481 gtggggcacc aggagtttac gtaggtgaaa aaaaagtcgc atcgatagga ctgagggtga
      541 aaaatgggtg tacctatcat ggtatagcat taaatgtgaa catggatttg agcccatttt
      601 taggcattaa cccatgtggt tttgctaaaa tggaaatgac acagatgagt cattttcatc
      661 ctaatattca gttagaggat gtaagccaac attttgtaca gtattttttg actcaattta
      721 aataatagga aattatgaat ttgttagcag attatgtgca accactgact tattggctac
      781 aagcaaaccc acattggtcc ctgtttatta ctttcttaat cgctttaagc gaatctttag
      841 ctgttattgg gagtattata cctggctctg ttaccatgac agcaataggg atattagcag
      901 gttctggaat catgcgtatt gatttgacat tacttgctgc tactctggga gctatttgtg
      961 gagatagcct tagttatttt ttgggatatt tctatagtga tcgtttaatt gagatttggc
     1021 cttttagaaa atacccgggc ttattgaagt atggtaaaga tttttttgca agccatggcg
     1081 gtaaaagcgt attaattggt cgttttgttg gcccattgcg ttctatcatt ccagtaattg
     1141 ctggaataat gcatatgaaa cagtggaggt tcctgatcgc aaatatagtt tctgctatcg
     1201 gatggtctat tttatatgtt atgccagggg tgctgattgg tgcagcaggg catgaattat
     1261 cagcagaggg agccacaagg ttttttatct tgatattggt cattcttgct gttatttggt
     1321 tgagtagcct cgttctcaaa tggttattta ttaagttaaa ttcatttttg agaagtaact
     1381 tgcatcgttt ttggttagaa attaagacaa gccccagatt atccaaagtg tattatgcta
     1441 tcacccctgt tcaggaagaa aatcattata atacggttgg tttattactt attgctctcg
     1501 tgattttttt tctcttttta actttagtta tttttaccat tgaaggtcaa ctgctagact
     1561 atattaatgt gcctgtgcat ttttttatgc aaagctttca cagttcctta ctggaagcag
     1621 ttttcattgt atgtgctcaa ttaacaagca aatcaacctt aactgggtta tttgtcgctt
     1681 cttgtatatg gtttatttat cataaaaatt accgagctac ggtttattta agcagcataa
     1741 ccctattgag cggtgttatt gctttatttt taactaaaat tattgatagc cccagaccgc
     1801 aagggctatt agttactttg cctggttcct cttttccatc tgttagtatt tccattgcaa
     1861 ctgcattgta tggttttgtt ttattttaca taaatagtaa gtattcctta ataaccagca
     1921 gttttaaaac aattattcta attgtgcttg gattaagtgg gtttgctaat atctatttag
     1981 gtgatttttg gttttctgat attttagctg cttatttgct gggagctgaa gtatgtttga
     2041 ttcattgttt ggtatataga aggtttagta cttctgttat aaagaatgaa catcctttaa
     2101 ttatgcttat atcactgctt atggttattg tatttgcgac agctgtttca acagttgtga
     2161 attttaaaga acaggttcat gatcatatgc cctatcacaa ggagtttact ttaacagaaa
     2221 aacaatggtg ggatcagcaa aaacctcttt tacctttgta caggttaagc agaattggaa
     2281 aacgaataag tctcttaaac attcaatacg taggtgatct ggatgtattg caaaataagt
     2341 tagaacaatt tggttgggaa ccacagagcg ataaaacctt ttttaccaga ttgttgatgc
     2401 gaatgaataa tgcaccatca aacgttaaac tgcctttact cactcaacta tatgaaaata
     2461 aaaagccagg cttgataatg acatatcaag ataagaaagc aaagttgatt tttgatttaa
     2521 ctatttggca atcgaactat tatttatctg agcctaaaag aattgtgtgg ataggctcca
     2581 ttcatcaaaa tatttttaat ttaaagcaaa aaaaacccaa gcataattta ccaccagtat
     2641 taaatccatt atcttatgta atccctgctc ttgatgactt tacccttaga aggattgtgc
     2701 tatctgacga tttggtaaag acatcagttt tccctactgc tccttttatt cttttgataa
     2761 aaactaaata gcagagcgtc attgattgca ggaagcatgt tctgatttat ttatattagg
     2821 cctggctagt aacccatgta aattactgat ataactatgt atgttcttaa taatttatat
     2881 aataagggat tgagtcaatg catatcttgg ctaaagttat tcactgtatc tttccattaa
     2941 ttgccctaac acttttaatt attggaatta aacgaaacgc gatttattat attatttctt
     3001 cgttatggat aagccttatt tcactagtca ttcattatca gacttccggt ggagagattt
     3061 taggtactta ttttaattat cttaatgcaa caatatatac catcaatttg ctcgttttgt
     3121 gtttatcttt ggtttgtgtt ttatggcatt taagtaatga taatgtagcg tttaaaattg
     3181 ttagcagttt gtttcaatca tttatagtca ttggagcttt attggttgta atcaatcttt
     3241 ggattaacgc ttattttatc gaaagcagat tgataggaac ccctgtgatg caggtagctc
     3301 ttttagagaa accggaatat tgtgattaca gatatatatt ttataaggtg gcgctggatg
     3361 gtactgttca ctatctttgc cctaatcatt acggtttaat accatcgatt ggtcgattat
     3421 ctataagtcc agattttata gcatcgcagt tatcaatacc aagcaaaaag caaatgctct
     3481 tattgcaaca aaaaaggagt taaattagca attttctata ttttaacaaa ttgttctgat
     3541 atttattatt tgatagtatg tgtgtaattt accagtatag tgcgtctatg aaacgatttt
     3601 tttcgatatt gtttattctt tactgctgta atttatttgc aatggcacct aagcttaact
     3661 gggataaagc gattgacaaa gcaataaaac gttatgggct aagagtagaa ccgcaattaa
     3721 tttcttattt ttctaaagcc ggggttagct accctcctaa agaaattgcc ttgcttgcct
     3781 tcaagtcaga gcgtaaagtt gagttatggg caaaaaatcc aagccaggaa tggacgcata
     3841 ttcatgatta cccattaact ggttttagcg ggcgtttagg acccaaatta cgtgagaatg
     3901 ataaacagat ccccgaaggg atctataaat tagtaaattt taatcctttt agcagcatgc
     3961 acttatccat gatgattaac tacccaaata gtttcgatcg gcaaaaaggt tatatggacg
     4021 gtaggaaaga gttagggaat aatattttta ttcatggaaa aaatttatct gtcggatgtt
     4081 tggctgtcgg tgaccttgcc attgatcaac tgtttatctt ggcaagaaga gtcggcctca
     4141 aaaatattca agttattatt gcccctaatg acttacgata tca
//
LOCUS       DAXLDT010000222         4141 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXLDT010000222 DAXLDT010000000
VERSION     DAXLDT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.223"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            103..1467
                     /locus_tag="ACF2SP_002897"
     CDS             103..1467
                     /locus_tag="ACF2SP_002897"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360659.1"
                     /translation="MQLFNKDELNKELDPIDTSYSSVCSKTSLPVLDALLAKACKDGK
                     SGILNKTAVFYVHHPLKTSINVIDALIHLGAKPEHLFVLGKRYSECESVVSALVQKGV
                     HYQPCSMQTGIGQYSYSFIRDINWLWAKVVEKLDAQVEQILIMDHGGHAITYLPLMLL
                     EHYKIIGIEKTTAGFINSKKQGLPPIPVIDVANSAAKRFLESPLIADAVVNKIVSYLP
                     ENINDFVFGVVGLGSIGKAVVNRLHQLGCRLVVHDIDSAQQVFIDNKKNIIFTNEMSA
                     LIASADYIFGCSGRDISEGQLEQLRLATRNKTLISCSSEDKEYLSLLQMINQQAPALF
                     DPMDDIEFKTELGASIHLIRGGFPVNFDQTGESVPANDIQLTRSLVLAAVLQASDYLN
                     NTPKECGVYALDAAYQRFVIYEWFKHQDESRFDSEQLSCFLDEDWLANHSNGLNSRAI
                     QRIS"
     gene            complement(1485..1946)
                     /locus_tag="ACF2SP_002898"
     CDS             complement(1485..1946)
                     /locus_tag="ACF2SP_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFD2360660.1"
                     /translation="MNPWFTTLPWVLYLNFKTKACFHSARFEKATRYYVIRLEQDLLG
                     DWTLCVSNGRIKSRLGQSRIIAFECFADACEHFCLMAKERNQRGYHLVSYDTEDLLYQ
                     TMVLWACITEKPVTAQKRKTTTHKKRVRSPSRPTPEVQYPIHDISQMSLIF"
     gene            complement(1946..2830)
                     /locus_tag="ACF2SP_002899"
     CDS             complement(1946..2830)
                     /locus_tag="ACF2SP_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD2360661.1"
                     /translation="MALVGYARVSTIDQNLDIQLAALKSAGCKKIFSEKKSGTSKKDR
                     TALDECMEYIREGDTLVVTRIDRLTRSILDLQTLLHYLKDKEINLKALEQPVDTSNAS
                     GKFFLDMLGVFAEFETNLRRERQLEGIERAKREGKYKGRKPTARSKTDEVMKLINQGY
                     TRTAIAKKLNMGIASVYRILKTHRQDNPEKTIPGSQGTQKIAVVEVWLRVENNNKFVR
                     GKNESRRQIEQYCFSSFDMVKKDKDGWEYILKVPYTNDKDLDKTLYDVMHEAESIADC
                     RNGFTEMSIIEPATGKSW"
     gene            3013..3210
                     /locus_tag="ACF2SP_002900"
     CDS             3013..3210
                     /locus_tag="ACF2SP_002900"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFD2360662.1"
                     /translation="MKSAEIIKIIERDGWVKCHQKGSHCQFKHPTKKGRVTVPHPKKD
                     LPVKTVASIFKQAGINKEDIK"
     gene            3207..3611
                     /locus_tag="ACF2SP_002901"
     CDS             3207..3611
                     /locus_tag="ACF2SP_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFD2360663.1"
                     /translation="MRYPVVIHKDEHSDYGVSVPDIPGCYSAGATYDEALTNVIEAIE
                     CHLEGLLMDNESIPVGSTIDHWINEEEFQGGVWAFIDIDLSQISGKSKRINITIPERV
                     LNLIDLYSKNHAVKNRSSFLADAALSYIESHK"
     gene            3636..>4141
                     /locus_tag="ACF2SP_002902"
     CDS             3636..>4141
                     /locus_tag="ACF2SP_002902"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HFD2360664.1"
                     /translation="MSRNIIEKNIGAFLELKSSEVLAVYGSWGVGKTYIWNDVIKNKR
                     TMIPDCYKSYSYVSLFGINSLASLKTSVFENMISTDLIGENEPSIKTFISNLSSTIEM
                     RKKGYWNKKAPTIAKCLPWIKSFSPLIDHAMSMAIKDAVICIDDIERKGESLTVNEIL
                     GYINFLKE"
BASE COUNT         1288 a          715 c          837 g         1301 t
ORIGIN      
        1 tattaccaat gcaaaataat ttctgtctaa aaaatgttgt atatgatgaa aaaataaact
       61 aaaataaggc tcttttggtg gtctaactaa caggggcaat tgatgcaatt atttaacaag
      121 gatgaattga acaaagaatt agaccctatt gatacaagtt acagctctgt ttgttccaag
      181 acttccttgc ctgttttaga tgcacttctg gcaaaggctt gcaaggacgg taaatcgggt
      241 attttaaata aaaccgctgt tttttatgtt catcatcctt taaaaacttc aattaatgtc
      301 attgatgcat taattcacct gggagcaaaa ccggaacatc tctttgtttt aggaaagcgc
      361 tattctgagt gtgagtctgt tgtttctgct ctggtacaaa aaggggttca ttatcagcct
      421 tgttcgatgc aaactggaat agggcaatat tcatatagct ttattcgcga catcaattgg
      481 ctctgggcaa aagtggtaga aaaacttgat gcgcaggtgg aacaaattct cattatggat
      541 catggcggac atgcaatcac ctatttgcct ttaatgctcc ttgagcacta taaaattatt
      601 gggatagaaa aaacaactgc tggatttatc aatagcaaga agcaaggact tccacccatt
      661 cctgttattg atgtagccaa tagtgccgcg aaacgatttt tagagtctcc tctgattgct
      721 gatgctgtag ttaataaaat agtgtcttat ttacccgaga atattaatga ttttgtgttt
      781 ggtgttgttg gtcttggctc aataggaaaa gccgttgtta atcgattgca ccaattaggt
      841 tgtcgattag tcgttcatga cattgattca gcgcagcagg tcttcattga taataaaaaa
      901 aatatcattt ttaccaatga gatgagcgca ctcattgctt ctgccgatta catttttgga
      961 tgcagtggtc gcgatatttc tgaggggcaa ttagaacaat taaggctagc tacccgaaat
     1021 aaaacgttga ttagttgttc ctctgaggat aaagagtatt tatcattact ccagatgatc
     1081 aatcaacaag cacctgcact gtttgatcca atggatgata ttgagtttaa gactgagctt
     1141 ggggcatcga ttcacttaat acgaggcggg tttcctgtta actttgatca gactggagag
     1201 tcagttcctg caaatgatat tcagttgacg cgctctttag tccttgcggc tgtactacaa
     1261 gcttctgatt atttaaataa tacgcctaaa gagtgtgggg tgtatgccct cgatgcagct
     1321 tatcaacgct ttgtgattta tgaatggttt aagcatcagg atgagtcaag atttgatagt
     1381 gagcaattat catgcttttt ggatgaggac tggcttgcaa atcactccaa tggtttgaat
     1441 agcagggcaa tccaacggat atcttaagtt tcgtaaaata aattttaaaa aatcagcgac
     1501 atttgagaaa tatcatgaat tggatattga acctctggag ttggtcttga tggtgatcta
     1561 actcgtttct tatgtgttgt agtctttctt ttttgtgcgg tcactggctt ttctgtaata
     1621 catgcccata gcaccatggt ttgatataga agatcctctg tatcatagga taccaaatgg
     1681 taaccgcgtt gatttcgttc ttttgccatg aggcaaaagt gttcacaagc atcagcaaag
     1741 cattcaaaag caataatacg gctttgtcct agtcttgatt tgatccgtcc attggatacg
     1801 caaagcgtcc agtcacctaa taaatcctgt tccaggcgaa tgacataata gcgtgttgcc
     1861 ttttcaaaac gggcgctatg aaaacacgct tttgttttaa agttaaggta aagaacccag
     1921 ggcagggtgg tgaaccaggg attcattacc aggatttacc tgttgcaggt tctatgatgc
     1981 tcatttcagt aaatccattg cgacaatcgg caatcgattc ggcctcatgc atcacatcgt
     2041 aaagagtttt atccaagtct ttatcattgg tatagggaac tttcaaaatg tactcccagc
     2101 catccttgtc ttttttcacc atatcaaaag atgaaaagca gtattgctcg atttggcgtc
     2161 tggattcatt ttttccacgc acaaacttat tgttgttttc tactcgtaac caaacctcaa
     2221 cgactgcaat tttttgagtt ccttggcttc ctggaattgt tttttctgga ttatcctggc
     2281 gatgagtttt aaggatacga taaacagagg caattcccat gttgagtttt ttagcaatcg
     2341 cggtgcgtgt gtacccctga ttgattaact tcatgacctc atcggttttt gagcgtgcag
     2401 tgggtttccg gcctttgtac ttgccttcgc gcttggcacg ctcaatgcct tcgagctgtc
     2461 gttcacggcg aagattggtt tcaaactcag caaaaacacc gagcatatcc aaaaagaatt
     2521 taccggaagc attagaggta tccacgggtt gttctagcgc ttttagatta atctccttat
     2581 ctttcaaata atgcagtaag gtttgcaaat ccaaaatgct tcgggtcaat cggtcaattc
     2641 gggttactac caaagtatcg ccttcacgaa tgtactccat acattcatcc agagcagttc
     2701 ggtctttttt tgaagtacca ctttttttct cggaaaaaat ttttttgcat ccagcgcttt
     2761 ttaaagcagc aagttgaata tccaaatttt ggtcaatggt ggaaacgcga gcataaccaa
     2821 caagcgccat gaattttcct atcaataaaa tttaaacaca gtgatattac cgtatcataa
     2881 ataaaaacaa aactctattg atagaaaaaa aagaggggtt taagaagagg taaaaattat
     2941 cacaagggtg tacccttgtg atattaatct cgctttcttg aatatgtgtg tatagtgtgt
     3001 ataattaaag atatgaaaag cgcagaaata ataaaaataa tagaacgcga tggttgggtg
     3061 aagtgtcacc agaaagggag tcattgccaa tttaagcatc caacaaaaaa gggtagggtg
     3121 actgtgcctc atcctaaaaa agatttaccc gttaaaacgg tagctagtat ttttaaacag
     3181 gctggaatta ataaggagga tataaaatga gatatcctgt agtaatacat aaagacgagc
     3241 acagtgatta tggcgtaagt gtcccagata ttcctggatg ttattctgct ggagctactt
     3301 atgatgaggc gttaactaac gtcattgagg caattgaatg tcatttagaa gggttgttaa
     3361 tggacaatga gtccatacca gtaggttcta caattgatca ttggataaat gaggaagagt
     3421 ttcagggtgg agtgtgggca tttattgata ttgatttaag tcaaatatca ggtaaatcaa
     3481 aacgcatcaa tatcactatt ccagagcgcg tattaaatct tattgattta tattctaaaa
     3541 atcatgcagt aaaaaatcgc tcttcatttt tagcagatgc agcacttagt tatatagaaa
     3601 gtcataaata atacttatgg gttagataaa aaaccatgtc aagaaatatc atcgagaaaa
     3661 atataggtgc ttttttagaa ttaaaaagtt cagaagttct tgccgtatac ggtagttggg
     3721 gtgtaggaaa aacctacata tggaatgacg taatcaaaaa caaacgtaca atgattcctg
     3781 actgctacaa atcatattca tatgtgtcac tatttggtat caattcactc gcaagcctga
     3841 aaacatcagt ttttgaaaat atgatcagta ctgatttaat tggcgaaaac gaaccttcta
     3901 taaaaacctt tatcagtaat ttatcctcta cgatagagat gaggaaaaaa ggatattgga
     3961 acaaaaaagc cccaacgatt gcaaaatgtt taccttggat aaaatcgttt agtcctctta
     4021 tagatcatgc aatgagcatg gctattaaag atgcggtaat atgtattgat gacattgaaa
     4081 gaaaaggaga atctttaact gttaatgaga ttttgggata tatcaatttt ttaaaagaaa
     4141 a
//
LOCUS       DAXLDT010000223         4136 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXLDT010000223 DAXLDT010000000
VERSION     DAXLDT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.224"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            123..1220
                     /gene="lem23"
                     /locus_tag="ACF2SP_002903"
     CDS             123..1220
                     /gene="lem23"
                     /locus_tag="ACF2SP_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem23"
                     /protein_id="HFD2360665.1"
                     /translation="MKRKDEQNNLTKGKANEQNTVSTNTHLLFSKPPGINADTANHMA
                     SSIDRLKDFDSSKQSLWLKYSEEIINEFCALLPEFPKRKPKVREDIRCPEEALLTQAG
                     CAYAFLASKRREVAKLLKHGPLRHQFGLFNKSGFASIDDFNLSKDIPSQKDKIYEHLS
                     LLISQPNPFIEVKKNHREAEGFSIYTITFKMKPDEIEQVHKKNLDSNKPFIENDTVLT
                     ITFNDYGKNDNRTSIVIDHCPTPFAENYTSESSYFKVQFELIEPYFQACQTWEASDGI
                     DQFLENAGRIAYILARMQPVGRGNSAIVEWMIRGLAQIKNIELGPFSPDEKIGWDFKA
                     FLTPEIQDYAHWFSEKAFAHRQLKSVPSLGH"
     gene            complement(1307..2167)
                     /locus_tag="ACF2SP_002904"
     CDS             complement(1307..2167)
                     /locus_tag="ACF2SP_002904"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2360666.1"
                     /translation="MYRAKLSARFFIGLLVLLMFNTGNAQSLWLTLPPTPTLPNAEKS
                     GYASVNGIKIWYAVFGQGQPLILLHGGLANSNYWGKQVPVLAKKFQVIVMDSRGHGRS
                     THDSKPYSYDLMASDVISLMDFLKIKKAAIVGWSDGAIIGLNIAINHPERLIKLFAFG
                     SNSNPQGVKSSAGAVFNEFALVRSKKEYEKLSPTPTKYGEFLSQIKKMWETQPNFTKT
                     QLNSIKIPTWIVDGDHDEVIKRENTEFIAAQIPNAGLLILPTVSHFAFLQDPTLFNSV
                     VMHFLEQKSE"
     gene            complement(2431..2736)
                     /locus_tag="ACF2SP_002905"
     CDS             complement(2431..2736)
                     /locus_tag="ACF2SP_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2407 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360667.1"
                     /translation="MYNVVSFNNEGVEHMKPTNEKSKEIRKKYKAPGASDEQLANSIK
                     VASEFEYKNSSTFGWGGVYMVDRFQSMITGRAITNNTQRLLDAQALEEEASKSNLKQ"
     gene            2959..3618
                     /locus_tag="ACF2SP_002906"
     CDS             2959..3618
                     /locus_tag="ACF2SP_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360668.1"
                     /translation="MLTKNLNEALSAIKYVKSKLVYGSNTEKDSKKPNWEPGRVILLR
                     KEVHALAVVYARSHNGKAGFDVISKLTHAALLKYGFGNCGEQAQTAFCYLQSRGVAPL
                     DYCQTSIGEHCLIVIGRVSGSDPNDISTWGEDAVICDPWAEKAYPLSEFRAMQSPDND
                     IRYPEICYANGKSPKPYLAGELISHHRIEPASKASSLSFFKKESYEHSQLNSATPMLI
                     A"
     gene            3945..>4136
                     /locus_tag="ACF2SP_002907"
     CDS             3945..>4136
                     /locus_tag="ACF2SP_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2360669.1"
                     /translation="MTNSNHLRNPVTKSLNDLCQDELSVIGSYLGAWNDLWSLTFTSS
                     EMKKCLIHQQTDELWKFFFK"
BASE COUNT         1382 a          804 c          719 g         1231 t
ORIGIN      
        1 aataccagat tatggttggt aaaatttaaa aatactgttg cttattaata ctcatctagc
       61 atatcacatc ccgaaattat gcttgctata cttaatagta cacaacaagt tctacgggta
      121 ttatgaaaag aaaagatgag caaaataacc ttaccaaagg caaggcgaat gaacaaaata
      181 ctgtcagcac caatacccat ttgttatttt caaaaccacc tggtattaat gccgatactg
      241 caaatcatat ggcttcatct attgaccgct taaaagattt tgacagctca aaacaaagcc
      301 tttggttaaa atacagtgaa gaaatcatca acgaattctg tgctttatta cctgagtttc
      361 ccaagagaaa acccaaagta cgtgaagaca ttcgctgccc ggaagaggca ttattaaccc
      421 aggccggttg tgcctatgcc tttttagcca gcaaaagacg cgaggtagcc aagcttttaa
      481 aacatgggcc tctaagacat caatttggtt tatttaataa gtcagggttt gcctcaatcg
      541 atgatttcaa tctcagtaaa gatatccctt ctcaaaagga taaaatttat gagcacctct
      601 ccttgttaat tagtcaacct aatcctttta ttgaagttaa gaaaaaccat agagaagcag
      661 aaggtttttc tatttacacc ataacattca agatgaagcc tgatgaaata gaacaagtgc
      721 acaaaaaaaa tttggattca aacaaaccat ttattgaaaa tgacacggta ctgacgataa
      781 ccttcaatga ttatgggaaa aatgacaaca gaacctcaat tgtcattgat cactgcccta
      841 ctccttttgc tgaaaactat acgagcgaat ccagctattt caaagtgcaa tttgaattga
      901 ttgagcctta ctttcaagca tgccagacct gggaagcttc tgatggaatc gatcagtttt
      961 tagaaaatgc cggaagaatt gcttatatac tggctcgtat gcaacctgtt ggacgtggta
     1021 attctgcgat tgttgaatgg atgattagag gccttgccca aatcaaaaac atcgaattag
     1081 gaccattcag ccccgacgaa aaaattgggt gggattttaa agcgtttctg actcctgaaa
     1141 ttcaggatta tgctcattgg ttttccgaga aggcctttgc gcataggcaa ttaaaaagtg
     1201 ttccttcttt gggacattaa actaaattga gcaaactacc ttgtgcagca acagtgcaga
     1261 accttttctc ctcttaatta caggtatcta tgcaacgtga gcaaattcat tccgatttct
     1321 gctcaagaaa atgcataaca acactattaa aaagagtcgg atcttgaaga aatgcaaaat
     1381 gactaactgt tggcagaatg agtaaaccag cattcggtat ttgcgctgct ataaattccg
     1441 tattttcgcg ctttattact tcatcatgat caccatctac tatccatgta gggattttga
     1501 ttgaattaag ctgggttttc gtaaaattcg gctgtgtttc ccacattttt ttaatttgag
     1561 ataaaaattc tccatatttt gttggtgtag gcgagagctt ttcatattcc ttcttgctgc
     1621 gaactaatgc aaactcattg aaaacagcgc ctgcggaaga tttgactccc tgaggattag
     1681 aatttgaacc aaaagcaaaa agctttatta atcgttccgg gtgatttatt gctatattca
     1741 gtcctattat tgccccatca ctccacccga caattgcagc ttttttaatt ttcagaaaat
     1801 ccattaacga aataacatct gaggccatta aatcataact gtatggttta gaatcatgtg
     1861 tactacgacc atggccgcga ctatccatca caataacttg aaattttttg gccagaactg
     1921 gaacttgttt tccccagtag ttagaattag caagcccgcc atgaaggaga attaggggtt
     1981 gtccctgacc aaataccgca taccaaattt ttatgccatt aacagacgca taaccactct
     2041 tttctgcatt gggtaaagtg ggggttggtg gcaaagttaa ccataaagat tgagcattcc
     2101 ctgtattgaa catcaacaat accagtaacc caataaaaaa tcttgcactc aatttggccc
     2161 gatacattta gtcactgttc ctttagtttg tttaaaaaaa tcacataagc agcctactaa
     2221 gacatagcct atgttaccac ttaaaatgaa ctatatacta tagaaaagaa ttatagaact
     2281 atattgaata gattaaagag agtactcctc ttagagtata actacttaac tatgggataa
     2341 gcagggaaag tatgggctca gatcttgttt ttggactatt catattcata gattgaagaa
     2401 aaacgaaaag cctgacccct gataaatgag ttattgtttt aggtttgatt tagaagcttc
     2461 ttcttcaagt gcttgagcgt ccaataacct ttgtgtgttg ttagtgattg cacgacctgt
     2521 gatcattgat tgaaagcgat caaccatata tacaccaccc caaccgaaag tactgctgtt
     2581 tttatactcg aactcagaag ctaccttgat tgaattagcc agttgctcat cagaagcgcc
     2641 tggcgcttta tattttttac gaatttcttt acttttttca ttggttggtt tcatgtgctc
     2701 aacaccttca ttgttaaaac tgacgacatt atacatgaat aatattcaaa cacaatttat
     2761 ttaaaaaaaa caatttgatc caaatttttc acaaacattt ctttagtaaa aaactctcaa
     2821 ttacatgacc tttacaatca cttgagtttc caaaaaataa aatctcacaa tctaaaaaca
     2881 aacccaaata gataaaatat taacaaactg tattttattt gatataataa gtctgtttta
     2941 ccctctgaga tttttttaat gctcaccaaa aatctgaatg aagccttaag cgcaataaaa
     3001 tatgtaaaat ccaaactagt ttatggttcc aataccgaga aagattcaaa gaagccaaat
     3061 tgggagccag gaagagttat tctcttaagg aaggaagttc atgcactagc agtcgtctat
     3121 gctcgttcac ataatggcaa agcaggattt gacgtcatct ccaaattaac ccatgcggca
     3181 ctcttaaaat acggattcgg caattgcggt gagcaagcac aaacagcatt ttgttatctg
     3241 caatctcgag gagttgcgcc gcttgattat tgtcaaacat caataggaga gcactgcctg
     3301 atagtgattg ggcgcgttag tggttctgat cccaatgata tttcgacatg gggagaggat
     3361 gctgtaattt gcgatccctg ggctgaaaaa gcctatcctc tttctgaatt tagagcaatg
     3421 caaagtcccg ataatgatat tcgctatccc gagatatgtt atgctaatgg taaaagtcca
     3481 aagccttatt tggcagggga attaatcagt catcacagaa tagagcctgc ctcgaaagca
     3541 tcatcgctct ctttcttcaa aaaagaaagt tacgaacatt cacaattaaa tagtgctacc
     3601 cccatgctta ttgcttaaag cagtccagtg ccaaagtgca atgactacca tgtgtacacc
     3661 aattgctacc ttttgttgta acgaatcatc aaattatcct gacgctattg attaaaatct
     3721 ggcaaagaga aaaaacacac cttgtgcgag caaggaaaag ccaggatctc tgatgccctc
     3781 ttttacaaaa taattccaaa attatccagt aagaaaaatt aatccatcta ttgttaacta
     3841 ttaatattct cttaataaaa agaaagcatt atagtcatct ggaagtcaca tttgcagatc
     3901 aaaaaacata atagaaaaac aaaaccacaa tcaggaggta aaaaatgaca aacagtaacc
     3961 atttaaggaa tcctgttacc aaatcattaa atgacctttg ccaagatgag ctgtctgtaa
     4021 ttggctccta ccttggtgcc tggaatgatt tatggagctt gacgtttaca tcatcagaaa
     4081 tgaaaaaatg cttaatccat cagcaaaccg atgagttgtg gaaatttttt ttcaag
//
LOCUS       DAXLDT010000224         4068 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXLDT010000224 DAXLDT010000000
VERSION     DAXLDT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.225"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1875
                     /gene="ceg32"
                     /locus_tag="ACF2SP_002908"
     CDS             <1..1875
                     /gene="ceg32"
                     /locus_tag="ACF2SP_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HFD2360670.1"
                     /translation="KFINIIFNDGFSTTYDQQTFIKALSALDIEKAIDAYTNVKKKLE
                     NTQDIIANKEGGRNKLISELQTLIANKQEPYLSANEKLGGFYSKRKLSASEGFHLAYQ
                     ANRRDPIKPEVIENIITKMKPIDEDTHLDIHIRPPDCGVFITPEDIKKFQEAGIKVNI
                     TIHEYKQNYTRRYLQQYTHDLMRQANSVQFFNATDRENAIIAATYGDCDKRNTTEPTG
                     VAKKIREVGEDFDLDKYPVQKYDLKGKSGLTVASQKLSTEPDHPLDVVAKAPNILSFG
                     TIRPGKGFEEALKLAQLIKDNSLSIDEKIKRVPIVKLAGDPQDKALMKQIVIERFGKT
                     AVKTYQKTHPYDNRFNNSQRRDYWKNLVRELNAKVKEEGAVLNNPYIEIYPWCEPHEL
                     LDLKQNCKYVCRMDDMGMRNNGSAIISVLDVGVVYTKFGSVTDDIFIKGGKYGNAVDI
                     GEYRYGKYSLLKKEKEFKEQHEEEPLPKWLIKNPDSAYKRQSESRDPKDILDSIVARE
                     ENQLICDNIEDSDNYRTVVEAQKLLKERFTLKNAVDHLLENIGLGHLIAQEEVDELFE
                     TVDPVQAQIDNLDVLSDIKTPRLCLSRSCPELGFFGSRRGDLETKQENNKLKESILVF
                     "
     gene            1877..2767
                     /gene="mesI"
                     /locus_tag="ACF2SP_002909"
     CDS             1877..2767
                     /gene="mesI"
                     /locus_tag="ACF2SP_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector metaeffector MesI"
                     /protein_id="HFD2360671.1"
                     /translation="MVKGKLMPNFMKDVQKLICDEGITDNVIMTLSRLKPFDLAMLKA
                     TSDDKVKTLLDSDELKPFWVNKFNKLRLEKDHIFQFRNPDPQSRADFYCGYVLYLAVL
                     KEKQNEVSSYSDYLNLSFTTFNCFYAAQEILTFLIGACKNDTKRENIDLLYNFVTSRS
                     TQIQEHKTPGCLLLANAYFYLAGFYLRLDLKAESIECYKECWGQLHLAQLLETDSERE
                     IHNAYFNKGLATSNAFGLNSISEIKARCLELASEALPYPVRNVIEANAVNTFESRFKN
                     SMSMAGKEDEEENMPRPTIL"
     gene            3173..>4068
                     /locus_tag="ACF2SP_002910"
     CDS             3173..>4068
                     /locus_tag="ACF2SP_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFD2360672.1"
                     /translation="MQRLKNIFTAIYQYLLKETEDASHQITLFGIVMMINYPLFGVFW
                     KLEHFQLTEEFILRITAALLCACLAFNQFWPRQLLKFLPVFWYIVLLFCLPYFFAYLT
                     LINNGSTLWLMNCVSAIFFLLLVSSVLGALILLISGVGLAFFHFYILSNNQFVYIPGT
                     ISLFSLIVTFIAAIIIGALFARDREITYAGRLSGMRMLAGSIAHDLRTPLASIYLQAE
                     LQELIVERLNNPEVQKDLKENLSKITRGIEMSNQLIRMQLNNIQRDKLDTSTFSIYSI
                     KKLLKASLEEYPFKENQKSLIH"
BASE COUNT         1410 a          677 c          733 g         1248 t
ORIGIN      
        1 aaatttatca atattatttt caacgatggg tttagcacca cttatgacca acaaaccttt
       61 atcaaggcct tatcagctct tgatattgaa aaggcaattg acgcttatac aaatgttaaa
      121 aagaaactgg aaaacactca ggatattatc gccaataaag aggggggtag aaataagtta
      181 atttcagaat tacaaacttt aatagcaaat aaacaagaac cttacctctc tgcaaatgaa
      241 aagcttggcg gtttttacag taaaagaaaa ctctctgcaa gtgaaggatt tcatttagcc
      301 tatcaggcaa accgacgtga cccgataaaa cctgaagtga ttgaaaacat tattaccaaa
      361 atgaaaccca ttgatgagga tactcatctt gatattcata ttcgcccacc agattgcggg
      421 gtctttatta ctccagaaga catcaaaaaa tttcaagagg ctggtattaa agtcaatatc
      481 acaatccatg aatataaaca gaactacact cggcgttatt tacaacaata tactcatgat
      541 ttaatgcgcc aggcaaacag cgtgcaattc tttaatgcaa cagataggga aaatgctatt
      601 attgccgcta cttatggtga ttgtgataag cgcaatacaa ctgaaccgac tggtgttgca
      661 aaaaaaataa gagaagttgg ggaagatttt gacttagata aatacccggt tcaaaaatat
      721 gatttaaaag gaaaatcagg attaaccgtt gcatcacaaa aattaagtac cgaaccggat
      781 catccattgg atgtagtagc caaggcccct aatatcttat cttttggtac gattcgtcct
      841 ggcaaagggt ttgaagaagc gttaaagctg gctcaattga tcaaagataa ctctctttct
      901 attgatgaaa aaataaaacg ggtacctatt gtaaaactgg ctggtgaccc acaagacaaa
      961 gctttaatga aacaaatagt tattgaaagg tttggtaaaa cagcggtcaa aacttatcaa
     1021 aaaactcatc cctatgataa tcgattcaat aatagtcagc gcagagatta ttggaaaaat
     1081 ttggttaggg agttaaatgc aaaagttaaa gaagaaggcg ctgttcttaa taatccttac
     1141 atcgaaattt atccttggtg tgagccgcat gaattgctgg atttaaagca aaactgtaaa
     1201 tacgtttgtc gaatggacga catgggcatg agaaacaatg gctcagcaat tatcagtgta
     1261 ttagacgttg gagtcgttta tactaaattt ggttctgtta cagacgatat tttcattaag
     1321 ggcggcaaat acggcaatgc cgttgatatt ggagaatatc gttatggtaa atacagtctg
     1381 ttaaaaaaag aaaaggaatt taaagaacaa catgaggaag aaccactacc taagtggcta
     1441 atcaaaaatc cagactctgc ttataagcga caatcagaat cgagagatcc aaaggatatt
     1501 ttagactcca ttgtcgcaag agaagaaaat caattaatat gtgataatat agaagattcc
     1561 gataattatc gaacagttgt cgaagcgcaa aagttattaa aagagcgctt tacactcaaa
     1621 aatgctgtag atcatttatt agagaatata ggactcggcc atttaattgc tcaagaagaa
     1681 gtcgatgaat tatttgagac agttgatcca gtgcaagcac aaatagataa tttagacgtt
     1741 ctttctgata ttaagacacc acgtctgtgt ttatcacgct cttgccctga gctaggtttt
     1801 ttcggctcac gtcgtggtga cttggagact aaacaagaaa ataataaact taaagaatcg
     1861 atactggtat tttgatatgg taaaaggaaa acttatgccc aattttatga aggatgtgca
     1921 aaaattaatc tgtgatgaag gcatcacaga taatgtgatt atgactctat ccaggttaaa
     1981 accatttgat ttagccatgc tcaaagccac tagtgacgac aaagttaaaa cgctactgga
     2041 tagtgatgaa ttaaaaccat tctgggtaaa taaatttaat aaattgcgat tagaaaaaga
     2101 tcatattttt caatttagaa acccggatcc gcaatcaaga gctgatttct attgtggata
     2161 tgttttatat ttagcggtat taaaggaaaa acaaaatgaa gtcagtagtt attctgatta
     2221 tcttaatctt tccttcacaa catttaattg cttttatgcg gctcaggaaa ttttgacgtt
     2281 cttaattggt gcttgtaaga atgacaccaa aagagaaaat atcgaccttt tatacaattt
     2341 tgtaactagc cgaagcactc aaatacaaga acacaaaacg ccagggtgct tattgcttgc
     2401 taatgcgtat ttttatcttg ctggatttta tctgcgcctg gatcttaaag cagaaagcat
     2461 tgaatgttat aaagaatgct ggggacaact tcatttggca caattattag agacagattc
     2521 tgaacgagaa attcataacg catactttaa taagggatta gcgaccagta atgcatttgg
     2581 tttgaattcc atatcagaaa ttaaagctcg atgccttgaa ttagcctcag aagcattacc
     2641 ctatccggtt cgaaatgtca ttgaagcaaa cgctgtaaat acctttgaaa gtagatttaa
     2701 aaacagcatg agcatggctg gtaaagaaga tgaggaggag aacatgcctc gaccaactat
     2761 tttatagtag agtccgagaa cagtcagctg atattcttct tatggcgttg tgttataacg
     2821 ccaaaaccaa acaagatgct cttccaggcc tctgtatttt gcataggaca attcatgtta
     2881 tattaaagcg caataatatg agtttttact tatggaaaag taatattagt attaaaaaac
     2941 agggattatg gtatccttgg tatttgtttt aattgggagc aatattattt aacttaagtc
     3001 caagaaaccg tagaaatcat aactctaatt ggtagcatcc attcagagtt caccattgaa
     3061 atactcataa tttacaagta tttctaatcc tacactaact gttcaatttt caggactaat
     3121 aagattaata cccaaacaat ctcaaagttt ttactcagat aattaaggaa tcatgcaaag
     3181 gttaaaaaat atatttactg caatttacca atatttatta aaagaaactg aagatgcaag
     3241 ccaccaaatc actttatttg gcatcgtcat gatgattaat taccctcttt tcggagtttt
     3301 ctggaaactg gagcattttc aactgacaga agaatttatt ttacgcatca ctgcagccct
     3361 cctctgtgcc tgtttggcat ttaatcaatt ttggccaaga caattattaa aatttctacc
     3421 tgttttttgg tatatagttc ttctcttttg tttgccttac ttttttgctt atttgactct
     3481 cataaacaat ggctcgactc tttggctaat gaattgcgta tctgctatct tttttctttt
     3541 attagtttcc agtgtattgg gggctcttat attacttata tcgggtgttg gacttgcttt
     3601 ttttcatttt tatattttat caaataatca gtttgtctat atcccaggaa ccatatcact
     3661 ttttagttta attgttacgt ttattgcagc aataatcatt ggcgccttgt ttgcacgaga
     3721 tagagaaatt acctatgccg gacgtttatc gggtatgcgc atgctggctg gaagtatcgc
     3781 tcatgattta agaacgccgc ttgccagtat ttatttacaa gccgaattac aggaattgat
     3841 tgttgaaaga ttaaataatc cagaagttca aaaggacctg aaggaaaatt taagcaaaat
     3901 tactcgcggc atagaaatga gtaatcaatt aattcgtatg caattaaata atattcaacg
     3961 agacaaatta gataccagta cattttcgat ttattccatc aaaaaattgc tcaaagcgtc
     4021 tttagaagaa taccctttca aagaaaatca aaaatcattg attcattt
//
LOCUS       DAXLDT010000225         4049 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXLDT010000225 DAXLDT010000000
VERSION     DAXLDT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.226"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..276)
                     /locus_tag="ACF2SP_002911"
     CDS             complement(<1..276)
                     /locus_tag="ACF2SP_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360673.1"
                     /translation="MITSVSFGEISKRNEGRVKNIVYLDFDGTITGAHGREVISSPLC
                     EALSNKATFDERMRYKNEYDASDNKVKITENAKKFLQNVNKLHPQVKI"
     gene            complement(1365..>4049)
                     /locus_tag="ACF2SP_002912"
     CDS             complement(1365..>4049)
                     /locus_tag="ACF2SP_002912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360674.1"
                     /translation="ALSRASYPEALLADIEAIATGTLAHTPAVLTPSRITDDASSLYA
                     TRLLASTPDAPESAIRAVVENPGFNPDAKDNNRLTPFYLAAESGHIPLASVLRERGAN
                     YLVKFLNQYSSLYVAVENNHLPFLRWFLGALSVEQRVELLVVQDQHRNTVLHKTLSRP
                     ELLKLLLEGLSLEQRVELLKVKGSDGNIVLHTVASHPKPLKFLLEGLSVEQRVELLKV
                     KGSDGNTLLHFAASNPKSLKFLLEGLPEESRVELLTTLRRKDGASVLDFAATHPKSLK
                     FLWESLSEEKRIEVLEMNDEHGNTVLHWAVSRPKSLELFLERLSDEQRFKLLWMKVQF
                     GNTVLHLAANSPETLKFFLEGLPEEKRIEVLTILNQYGDTVIHRAAHFPASLKFLWEG
                     LPEEKRIELLRMKGNYNNTLLHNAASHPESLKLLLEGLSEQQRAELLRINGSIGNTVL
                     HCALGYLESLKFLLEGLSEESRFQLLGMKNQDDNTVLYLAASRPESLKLLLEDLSVEN
                     RVQLLGMKNQYGNTVLHEALSQPKSLKLLLEGLSEESRFQLLRMKNQYGDTVLHEALS
                     QPKSLKLLLEGLSEESRVQLLGMKNQDGYTVLHKALSLPESLKLLLEGLSEESRVQLL
                     GMKNQDGYTVLHKALSLPESLKLLLEGLSEESRVQLLGMKDYHGNTLLHEALSRPKSL
                     KFLLEGLSEESRFQLLGMKNQDGNTVLYLAASRPESLKFLLEGLSEESRVQLLGMKDK
                     YGNTALRCAVPHPASLKFLLEGLSEEKRLELLSAKNQHGDSVLHRALSCYPESLGVIL
                     EMLCETTQRQVLEDLGCLRGNVFLGKPEILEEINFALKFSVSVSNTSLSCLDTSSQAA
                     TSSGNQNTFFDSGSKRRIDAQRNVSPEETEERACSLKK"
BASE COUNT         1127 a          897 c          819 g         1206 t
ORIGIN      
        1 aatttttacc tgtggatgta atttattgac attttgtaga aatttcttgg cattttctgt
       61 tattttaacc ttattgtcag atgcatcata ctcattttta tagcgcatcc tttcatcaaa
      121 agtcgcttta ttgctcaaag cttcacacag aggggagcta atcacttctc tgccatgtgc
      181 accagttatt gttccatcga aatccagata aacgatgttc tttaccctac cttcatttct
      241 ttttgaaatt tcaccaaaag acacggacgt aatcatgatt atttcctcgt aaaaccaacc
      301 tatgcataca tttaatcatg atgtttattt tgtgtacaat tcattatttt cataagttat
      361 attgttttta ataatatcta ttatttttca aaattttaat gattactcct atgcttgcgt
      421 tgagctaaat ataggttgca tggaaagaaa aattaatcaa cagtgccaaa ttaaaaggag
      481 aaacacgatg agattaataa actaaagtac atggcaattt attttattaa ccattatgaa
      541 acggtgaaaa ctacccccag gtaagattga taacatatct gtgatccgta tatcaggcct
      601 tgcaataaag gctgataacc acttacttta atctatcaag ttggggtata ttctaaccag
      661 gctttttctg tatctcttta tcatcttctt gctttaaaca gcgttcttga taaaaaaata
      721 tagaccatca atatcaatat attgaactgt ttttgatcat atataatgaa aattctttgc
      781 ctctataaat gatagagtca ggttagaact tattcaataa tactgctcag acaactgtat
      841 gtctttaagc gaaaatttaa tgagagttaa ctgagaagca ttgtgatttg tattttggcg
      901 ggttatatct atggcctcgc catcttcatc aatagcagtt acaacacatt gtttttctaa
      961 taaaaaacag tatctctacc tgttccacaa ccaaggtcta atgcctttat aggttgaaca
     1021 tgagactctt attcatccaa aatttcgtca tcagattctt caatcgaaaa aaatttttat
     1081 aaatttcctt aagatcttgc ttggcagaac attttctctt ggaaaaacga ctataagctt
     1141 cctcgactac cttataggct ttactatttc gatttgctgc aaaaaaataa agaccatcta
     1201 cccatatcct cccactacct gggtatcatg cttaatactt gattttagca agacatctta
     1261 ttgagttatg tgagaaccta aaaaaattac tgctctggca aatatatcca tcaggttgca
     1321 attaattttt ccttacaatg caacctgtat ttaagtgtca acgtttattt tttgagagag
     1381 caggcccttt cttcagtttc ctctggcgat acattccttt gtgcatcgat tctgcgtttg
     1441 gaaccgctgt cgaaaaacgt gttttgattg ccagatgaag ttgctgcctg tgatgatgta
     1501 tcaagacaag ataatgaggt gttgctgacg gatacagaaa acttcaaagc aaagtttatt
     1561 tcctctagga tttcaggctt acctaagaat acattgccac gcaaacatcc caaatcctcg
     1621 agcacttgcc tctgagttgt ctcacacagc atttctagaa tcacccctaa agattcagga
     1681 taacatgata aagccctatg caatacggaa tcgccatgct ggttcttcgc gctaagcagt
     1741 tccaatcgtt tctcttcaga caaaccctcc aaaaggaatt tcagggatgc ggggtgaggc
     1801 acagcgcagc gcagtgcggt atttccatat ttgtccttca tccccagcag ttggactcgg
     1861 ctctcttcag acaagccctc caaaaggaat ttcagggatt cgggtcgaga cgcagcgagg
     1921 tacagtacag tattgccatc ctggttcttc atccccagca gttggaatcg gctctcttca
     1981 gacaagccct ccaaaaggaa tttcagggat tttggtcgag ataaagcctc atgcagtaaa
     2041 gtattgccat gatagtcctt catccccagt agttggactc ggctctcttc agacaacccc
     2101 tccaaaagga gtttcagaga ttcgggtaga gataaagcct tatgcagtac ggtatagcca
     2161 tcttggttct tcatccccag cagttggact cggctctctt cagacaaccc ctccaaaagg
     2221 agtttcagag attcgggtag agataaagcc ttatgcagta cggtatagcc atcttggttc
     2281 ttcatcccca gcagttggac tcggctctct tcagacaacc cctccaaaag gagtttcagg
     2341 gatttgggtt gagataaagc ctcatgcagt acggtatcac catattgatt cttcatcctc
     2401 agcagttgga atcggctctc ttcagataaa ccctccaaaa ggagtttcag ggatttgggt
     2461 tgagataaag cctcatgcag tacggtattg ccatattggt tcttcatccc cagcagttgg
     2521 actcggttct ctacagacaa gtcctccaaa aggagtttca gggattcggg tcgagacgca
     2581 gcgaggtaca gtacagtatt gtcatcctgg ttcttcatcc ccagcagttg gaatcggctc
     2641 tcttcagaca aaccctccaa aaggaatttc agggattcca gataacctaa tgcgcagtgc
     2701 agtacagtat tgccaatgct accatttatc ctcagtagtt cggctcgttg ctgttcagac
     2761 aaaccctcca aaaggagttt cagggattcg ggatgagatg cagcgttgtg cagtaatgta
     2821 ttgttataat tgcccttcat cctcagcagt tcgattcgtt tctcttcggg caaaccttcc
     2881 caaagaaatt tcagggatgc ggggaaatgt gcagccctgt gtattacggt atcgccatat
     2941 tggttcagga ttgtcagcac ttcgattcgt ttctcttcag gcaaaccctc caaaaagaac
     3001 ttcagggttt cggggctatt tgcagccaag tgcagtacag tattgccaaa ttgaaccttc
     3061 atccacaaga gtttaaatcg ttgctcgtca gacaaacgtt ctaaaaagag ctccagggat
     3121 ttgggcctag atacagccca gtgcaagaca gtattgccat gttcgtcatt catctccagc
     3181 acttcgattc gtttctcttc ggacaaactt tcccaaagga atttcagaga cttggggtga
     3241 gtcgcagcga agtccagtac gcttgcacca tcttttctcc gcaacgtagt aagcagttcg
     3301 actcggctct cttcaggcaa gccctctaag agaaatttca gggatttagg gttagatgca
     3361 gcgaaatgca gtagggtatt gccatcgcta cccttcactt tcagtagttc gactcgttgc
     3421 tctacagaca agccctccaa aaggaatttc aggggtttgg ggtgagatgc aaccgtgtgc
     3481 agtacgatat taccatcgct acccttcact ttcagtagtt cgactcgttg ctctaaagac
     3541 aaaccctcca aaaggagttt cagtaattcg gggcgagata aagtcttgtg cagtacagtg
     3601 ttgcgatgtt ggtcctgcac cacaagcagt tcgactcgtt gctccacgga caatgcgcca
     3661 aggaaccatc gtaaaaaagg cagatgatta ttttctactg caacgtacaa cgaggaatac
     3721 tggttaagaa acttcaccag ataattcgca ccccgttcgc gaagaacact tgccagcggg
     3781 atatggccag actctgcagc aaggtagaat ggagtaagac ggttgttgtc tttggcatcg
     3841 ggattaaatc ccgggttttc aaccacggct cgaatagcgc tttctggtgc atcaggtgtt
     3901 gaagcaagta atcttgtcgc gtaaagactg gatgcatcat cggttatccg cgacggagta
     3961 agtactgctg gtgtgtgtgc taaagtgcct gtggcaatgg cctcaatgtc tgcaagcagt
     4021 gcttcgggat aggaggcacg cgaaagcgc
//
LOCUS       DAXLDT010000226         4043 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXLDT010000226 DAXLDT010000000
VERSION     DAXLDT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.227"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            184..540
                     /locus_tag="ACF2SP_002913"
     CDS             184..540
                     /locus_tag="ACF2SP_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360675.1"
                     /translation="MSKFFTLLICISIGLLSFPTYAQKTIRLSPNETKTLKNSTLWTL
                     NATCNVQSSTQVNGKIKIIVLKNNGKVNGKSLSTGQGTSITIKNNSSISVSAESGTQI
                     NLINLGNQELQAVCST"
     gene            complement(603..1325)
                     /locus_tag="ACF2SP_002914"
     CDS             complement(603..1325)
                     /locus_tag="ACF2SP_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HFD2360676.1"
                     /translation="MNYQYSPNLKKKFLLAFTTLFIVQMFYFAPVRASTGDTQLWTLM
                     NAIGPINKQHRKIRYWLEIQDRVGENISQLSQLLLRPGLGYELLPTTTIWLGYDRVYT
                     AQPFSNPPVNENRVWQQLLWSKEYTRLRLTARSRLEERFIQRAIHTAWRYRQLFKTSI
                     FIPKHPKYAAVISDEAFFQLNNFNLQRNQGFDQNRLFVGLGYRTSKNSTIEIGYLNQM
                     IKRVNNPDYRGDYLSTALLLTE"
     gene            complement(1539..2282)
                     /locus_tag="ACF2SP_002915"
     CDS             complement(1539..2282)
                     /locus_tag="ACF2SP_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2360677.1"
                     /translation="MVRLRIYLCSLLLAIPFYKPYALSLVIGTLSYNPPFETQVNDSS
                     KKNEFYGFEIELMDEICERINAKCQYKPLLFEQILAEINSGAINLGIGSINITPERSE
                     RFLFSLPYKASYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTIDYLKVYFNNNIQFKY
                     YETFMDLLNGLTDPQVSAIVTNSDQAQYWASITPDCKLLGDSFPFGIGFGVVARLDQA
                     ELIEKINQALIEIENDGTYLKIYNLFFQP"
     gene            complement(2443..3966)
                     /gene="ankQ"
                     /locus_tag="ACF2SP_002916"
     CDS             complement(2443..3966)
                     /gene="ankQ"
                     /locus_tag="ACF2SP_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkQ/LegA10"
                     /protein_id="HFD2360678.1"
                     /translation="MIMAATIPHIETITFNDWFLAIIDTPGIPIDEIETRAAEGFKRK
                     LTERQFQQLTELIHLISFIKHRRFSNPILALRIYVDENTSSLSRTALVEAVRMLPPED
                     NKTYELVAYLAQKNKLERYTNITKVPPLQIYQQNGVFEQCDEWILLFELFGLTQATPS
                     DFIPAIAHLLIIKNFGIPDLRALSKFMSTAHKLGILSQNFMNNMTPHLCNGQIIEKYE
                     SLLLKLQINDLLNKEILETIYSLIKQLDALDAFFSLYHEELSHDSTLSFLREILPSFC
                     VMSRPSKESYDDQVSTNTPLHLAVIESDKANLERTLAFANRNLLIKSSYENTALLLAC
                     KLADKESARLILAKMQELGCDVNQKDHHGMTALHWANFYHFDELIRELEVAGADPKLK
                     AANGKTCEYFYQHQFTMNDLKLNGKEIIDGEFKLSECALTDIAFHMDKIALNLKLTTP
                     SEIAELYARDEMAQIRSSNRFYLFFKLFRPKLINWLEKQNGLEPQVTYRFSGQSSSA"
BASE COUNT         1268 a          691 c          736 g         1348 t
ORIGIN      
        1 ttaaaaaaaa tttttaattc ggtcatttag tttatctaaa ctccctcatt aaaaacattt
       61 tttgccttgg tcttggggtt tttcataagt cctggcttaa taaaatctta agtaaatatg
      121 tatataatgt atacatattg cattaaaata gttgggcctt ggattctgga gaaggaaagt
      181 agcatgtcta aattttttac tttgttgata tgtatctcaa ttggtttatt aagctttcca
      241 acttatgctc aaaaaacgat acgacttagc cctaacgaaa ccaagacgct aaaaaatagc
      301 actttatgga ctttgaatgc gacctgtaac gtacagagca gcacgcaggt taatggtaaa
      361 ataaaaatca ttgttttaaa gaacaatggc aaagtaaatg gtaaaagcct ttcaaccggc
      421 cagggaactt ctattacaat aaaaaataac agcagcatct cggttagtgc agaatcagga
      481 actcagatta atctgattaa cttgggtaat caagaattgc aagcagtttg ttctacttaa
      541 ttaaatcaat ttttctcaaa caaaccattg aaagtaatgg tttgttttct ttgtccgctt
      601 gtttattctg ttaataatag agccgtggat aaataatctc cgcgatagtc agggttattt
      661 acgcgtttaa tcatttgatt gagataccca atctctatcg tgctgttttt actggttcta
      721 taacctagac ctacaaataa acggttttga tcaaagcctt gattacgttg aagattgaag
      781 ttattcagct gaaaaaaagc ttcatcacta atcacggccg cgtatttagg gtgtttggga
      841 ataaaaatac tggttttaaa taattgtctg taccgccaag cggtatgaat agctctttgg
      901 ataaagcgtt cttccaaacg cgaacgagct gtaagtctta agcgagtata ttccttgctc
      961 cacaataatt gttgccaaac ccgattttcg tttacaggag ggttggaaaa tggttgcgca
     1021 gtgtaaactc tatcatatcc gagccatatg gttgttgtag gcaatagttc atatcctagg
     1081 ccagggcgta atagcagttg agacaattgc gaaatatttt caccaactct atcctgtatc
     1141 tcaagccaat atcttatttt gcgatgctgc ttattgattg gtcctatcgc attcatgagt
     1201 gtccataatt gagtatcccc tgttgatgct ctgacagggg caaaatagaa catttgtaca
     1261 ataaataaag tagtaaatgc gagcaggaat tttttcttta ggttggggga atattgataa
     1321 ttcattgttt aaaaagactt aaaaagttac tgcacatcag gtttgagaaa ccatcgtcaa
     1381 tattttttat tttattgttc aattttttta taaaatagct gagagtcatt ttttatagtg
     1441 ttgatagaat gatgccaacc ttttattgtt tctcagatta tagataaaaa tcaatagatt
     1501 tattaagcac tggagccctt tgtcggtcga aataatactt aaggttgaaa aaagaggtta
     1561 tatattttaa ggtaagttcc atcattttca atctcaatta atgcctgatt gattttttca
     1621 atcagttccg cttgatctaa tctcgcaaca actccaaatc ctattccaaa aggaaaactg
     1681 tcgcctaata acttacagtc cggtgtaatc gatgcccagt attgcgcttg atcgctatta
     1741 gtcactattg cgctaacttg cggatcagtc agcccattaa gtaaatccat aaatgtttcg
     1801 taatatttaa attgaatatt attgttaaag tagaccttta aataatcgat agtaggtgaa
     1861 cctaaatgaa ttcctatagt ctttcctttg agatcgtcaa ttttttttat attcgagtta
     1921 gatggtataa gaaactggtg atagctggct ttgtagggaa ggctgaataa aaatcgttcg
     1981 cttctttcag gcgtaatatt aatgctacca atgcctaaat tgattgcacc agaattaatt
     2041 tcagctaaaa tttgttcaaa taacaatggc ttgtattggc attttgcatt gatgcgctcg
     2101 caaatctcat ccatgagttc aatttcaaaa ccataaaatt catttttctt ggaagaatcg
     2161 ttgacttgag tttcaaacgg aggattgtaa gataaggtac ctatgaccaa actcagcgca
     2221 tacggtttat aaaagggaat agccagtagc aaagaacata aataaattct gaggcgaacc
     2281 atttaaatat cctttttata tagttattta ttattttagc cttatttttg aaaaataaaa
     2341 gaacggttag atttatattt tttcataaat cagggcactt taccttgcat aaaacagagc
     2401 aagtaaattg ccagttgtac cgggtttgtt aagttaatga tatcaagcag aagaactttg
     2461 ccctgagaaa cgataagtca cttgaggctc caggccattt tgcttctcta accaattaat
     2521 taattttggc cggaataatt taaaaaacaa atagaaccgg ttgcttgaac gaatttgtgc
     2581 catttcatcc cttgcgtaca gttctgcaat ctcggatggt gttgttaatt ttaaatttaa
     2641 ggctatttta tccatatgga aagcaatatc cgtcagcgca cattcactta acttgaattc
     2701 accatcaatg atttctttgc catttaattt taaatcgttc atagtgaatt gatgttgata
     2761 gaaatactca caggtttttc cattggctgc ttttaatttg ggatccgccc cagcaacctc
     2821 caattcccgg attagctcat caaaatgata aaaattagcc caatgcaagg cggtcatacc
     2881 atgatgatct ttttgattga catcacagcc taattcttgc attttcgcta aaatcaatcg
     2941 ggctgactct ttatcagcaa gtttgcaagc caataaaagt gctgtatttt cataagagga
     3001 ttttatcagt aaattgcgat tggcaaaggc taaggttcgt tccagattcg ctttatcact
     3061 ctcaattaca gccagatgca aaggcgtatt tgttgatacc tgatcgtcat aactttcttt
     3121 actggggcgg ctcattacac aaaaggatgg caaaatttct ctcaggaaag acagagtact
     3181 gtcgtgcgat agttcttcat gatagagtga aaaaaaagcg tcaagagcat ctagttgctt
     3241 gatcagtgaa taaattgttt ccagaatttc cttgtttaac aaatcgttaa tttgtaattt
     3301 aagcaataat gactcgtact tttctatgat ttgcccatta cataaatggg gtgtcatatt
     3361 attcataaaa ttttggctta atatgcctaa tttatgcgca gtgctcataa actttgaaag
     3421 tgctctgagg tcagggatac caaaattctt gatgattaat aaatgagcaa tggcaggaat
     3481 gaaatcgctg ggcgttgcct gggttaaccc aaacagctca aagagaagaa tccattcatc
     3541 acattgctca aatacgccat tctgctggta aatttgcaga ggaggtactt tcgttatgtt
     3601 agtatatcgt tccagtttgt ttttttgagc caagtaggct accaactcat aggttttatt
     3661 atcttcaggt ggaagcatcc tgactgcttc aaccaaagca gttcttgata gagagctcgt
     3721 attttcatca acatagatgc ggagagccaa gataggattg gagaaacgcc tgtgtttaat
     3781 gaaactgatt aaatggatta attccgttaa ttgttgaaat tgccgttctg tcaattttct
     3841 cttaaagcct tcagccgccc ttgtttcaat ttcatcgatg ggaattcccg gagtgtcaat
     3901 tattgccagg aaccagtcgt taaatgtgat agtttcaata tggggaattg ttgcggccat
     3961 aatcactcct taaataaatt gttaatatta taaacaaaaa gcattatttt gataacagtg
     4021 aaatgacctc ttgaaagtaa ata
//
LOCUS       DAXLDT010000227         3963 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXLDT010000227 DAXLDT010000000
VERSION     DAXLDT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.228"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            433..529
                     /gene="ffs"
                     /locus_tag="ACF2SP_002917"
     ncRNA           433..529
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACF2SP_002917"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            555..2225
                     /gene="dnaX"
                     /locus_tag="ACF2SP_002918"
     CDS             555..2225
                     /gene="dnaX"
                     /locus_tag="ACF2SP_002918"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFD2360679.1"
                     /translation="MSYLALARKWRPRTFSQLVGQEHINKALINALNQQRLHHAYLFT
                     GTRGVGKTSVARILAKALNCEKGITSEPCLHCDTCVAIEQGRYIDLIEIDGASKTRVE
                     DTRDLLDNIQYAPTNGRFKIYLIDEVHMLSQHSFNALLKTLEEPPSHVKFILATTDPQ
                     KLPVTVLSRCLQFNLKHLPAELINQQLQLILQEERLDFEIQALDILAQAARGSMRDAL
                     SLLDQAITSCETKLLASDVKILLGHTQQDYALLLLQALAKQEAPTMLVLSQQIIAEGG
                     NFQYVLDEMLNYLHQITIYQCLGDNNPLISPKAEIKELAKQFSAEDTQLFYQIGIKGR
                     EEMHLAPTLAIGFNMTMLRMYTFKPASHVTTPPLAFEQNNKTCSLIIDDNTPAQNPIG
                     IEPIPVTKATGNIVAYHQTQSLSTDDKKSANSGESMNCDWNDIIPQLKLSGLALNAIE
                     NAEFLGKKEREITLGVQKGHQSLFTPTTINRIETALAGYYKSPVKITLNSQDAVHASP
                     AQQKEKATQEKQEQAEAALHADPVFQQLKQEFSAELVKNSIVLLKDDL"
     gene            2259..2597
                     /locus_tag="ACF2SP_002919"
     CDS             2259..2597
                     /locus_tag="ACF2SP_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFD2360680.1"
                     /translation="MDINQNLGNLMKEAQKMQQRMQEAQQQLSQLVVSGESGGGMVTI
                     KMNGRHDVTEVKIKPTLMDEDIEMLEDLIAAAVNDAVRKIEKASKEKISQLTAGLNIP
                     TDLMGGKEGE"
     gene            2606..3199
                     /gene="recR"
                     /locus_tag="ACF2SP_002920"
     CDS             2606..3199
                     /gene="recR"
                     /locus_tag="ACF2SP_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFD2360681.1"
                     /translation="MDALSRLVEALRCLPGVGPKSAQRMVFHLLQHQRQRGLHLASCL
                     EQAMKHISHCQQCNNYTEQTLCTLCQNPNRDSTLLCVVESPADVSAIEQSNSFQGKYF
                     VLMGKISPLDGLGPDDIGLPKLKELIIREKIQEVILALSPSVESQTTIHFIHQLLKDE
                     TVNISQLAHGIPSGGELEFLDGNTISSALKNRAVINV"
     gene            3192..>3963
                     /locus_tag="ACF2SP_002921"
     CDS             3192..>3963
                     /locus_tag="ACF2SP_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HFD2360682.1"
                     /translation="MSKSIFILCRKKILVLLLLSLCFITSTASASFHRSLWPKWLVNA
                     PLSKQSISHQAWQRFLDHHVITNEEDINLVDYTNINEKELASLKEYIKNLSQIDIDNY
                     NRQEQLAYWINLYNALTVLTVANYYPIANIQEINISPGLFSVGPWGANIITIKNTNLS
                     LDDINNRIIRPIWNDPRTHYALNNATIGAPNLSKQAYQGSLLEQQLNDAAFKYINSLR
                     GVHVIEGKLIVSKLYDWYEEDFGGTKKYVIKHLLQFAKE"
BASE COUNT         1305 a          781 c          745 g         1132 t
ORIGIN      
        1 tcataaagca gaaagtctca ctttgtttgt gcaaaaatga agtggaaaaa tgcttttgtt
       61 tcacttaatt gctactgatg gatattgaag atggtatttg ataaatccta tttacttgat
      121 tacttgatta cttgattact tattacttat tacttattac ttattactta ttacttatta
      181 cttattactt attacttatt acttatatag tattgtttat aacttaaatt tctatttcct
      241 aatattttgt agcttaaaag gaaatattta ttcatgcgga ttggatatgt tctttattag
      301 ccctggttat ttaattactt ttttaatttc tatcctttag gaaagcagtt acactcaaat
      361 aataaaatag tcattagctc tagcgcagaa atattttttt tgttactctt cgctattcaa
      421 atatggtgac tctattggtc ccctcgcgac gatagattgt gaaccccgtc aggcccggaa
      481 gggagcagcg gtagcaattg atgcgggcgc cggggtgtgg ctcttagagt cgccgcccaa
      541 cttgtgaaac acgtatgagc tatctggcac ttgcccgtaa atggcgacca cgcacatttt
      601 cccaactggt tggccaagag catattaata aagcactgat caacgcgctg aatcaacaac
      661 gcttgcatca tgcttatcta tttactggta ctcgcggggt cgggaaaacc agtgtagccc
      721 gtattttagc taaagccctc aattgtgaaa aaggcatcac ctcagaacca tgccttcatt
      781 gcgatacctg tgttgccatt gagcaaggcc gttatattga cctcattgaa atagatggag
      841 cctccaaaac cagagttgaa gacacgcgag atttacttga taacatacag tatgcaccaa
      901 caaatggccg ttttaaaatt tatttaatcg atgaagtgca tatgctatcc caacatagct
      961 ttaatgcttt actaaaaaca ttagaggaac cgccttcgca tgtgaaattt attctggcaa
     1021 cgactgaccc acaaaaatta cctgttactg tcttatcgcg ttgtttgcaa tttaatctaa
     1081 aacacttgcc tgctgagttg ataaatcaac aattgcaatt aattctccaa gaggagcgct
     1141 tggattttga aatacaagcg ctagacatat tggctcaggc tgcccgcgga agcatgcgcg
     1201 atgcattaag tttattggat caggccatta ccagttgtga aacgaaattg ctggcaagtg
     1261 atgtaaaaat acttttggga catacccaac aagattacgc gttgctgctc ttgcaagccc
     1321 tggccaagca agaagcgcca accatgcttg tattaagtca gcaaatcatt gccgaaggag
     1381 ggaattttca atacgttctt gatgaaatgc ttaactatct gcatcaaatt accatatatc
     1441 aatgcctggg cgacaacaat ccattaatta gtccaaaagc agaaattaaa gaattagcaa
     1501 aacagttttc ggccgaggac acacaattat tctatcaaat aggtattaag ggacgtgagg
     1561 agatgcatct cgcccctaca ctggccattg gctttaatat gaccatgctg cgcatgtaca
     1621 cctttaaacc agcttctcat gtaacaacac ctccattagc attcgagcaa aataataaaa
     1681 catgctcact aattattgat gataatacac cagcgcaaaa ccctattgga attgaaccaa
     1741 tacccgttac taaagcgacg ggcaacatcg ttgcctatca tcagacccaa tcattatcaa
     1801 cagacgataa aaaatctgcc aactctggtg agtccatgaa ctgtgactgg aacgacataa
     1861 taccacagtt aaaattatcg ggtttagcac ttaatgccat agaaaatgca gaatttcttg
     1921 gtaaaaaaga gcgagaaatt acattgggag tacagaaagg gcaccagtcc ttattcactc
     1981 ccacaacgat taaccgaatt gaaaccgcat tggctggtta ttataaaagt cccgtaaaaa
     2041 ttactttaaa cagtcaagat gccgtgcatg cctctccagc tcaacagaaa gagaaagcga
     2101 cccaagaaaa gcaagaacaa gcagaggcag cattacacgc tgatccagta tttcaacagt
     2161 tgaaacaaga attttcagca gaattggtca aaaattctat tgtcctgtta aaagatgact
     2221 tataatactt aaactaacca taaatagacg aggctgtaat ggatattaat caaaatttag
     2281 gcaatttaat gaaagaagcg caaaaaatgc aacaacgtat gcaagaagcg cagcaacaat
     2341 taagccagtt ggtagtaagt ggagaatcag gtggtggtat ggttactatt aagatgaatg
     2401 gacgtcatga tgtaactgaa gttaaaatca aacctacctt aatggatgaa gatattgaaa
     2461 tgctggaaga tttaattgct gctgcagtca atgacgcagt gcgcaaaatc gaaaaagcat
     2521 ccaaagaaaa aatcagccaa ctgactgctg gtcttaatat tccaactgat ttaatgggtg
     2581 gtaaagaagg agagtaaccc tggctatgga tgctctaagc cgcttggtag aggcgcttcg
     2641 ctgtcttccc ggtgtgggac caaaatcagc acaaaggatg gtgttccatt tattgcagca
     2701 ccaaagacag cgaggtttac accttgcctc ctgtttggaa caggcaatga agcacattag
     2761 tcattgtcag cagtgtaata attacaccga acaaactctg tgtactcttt gccaaaaccc
     2821 caatcgagat tcaacattac tttgcgtggt agagagtcca gcggatgttt ctgccataga
     2881 acaaagcaat tcctttcaag gtaaatattt tgtccttatg ggaaaaattt ctcctcttga
     2941 tggtttggga cctgatgaca tagggctacc gaaacttaag gagttaatta ttagggaaaa
     3001 aattcaggaa gttattcttg cattaagccc cagtgttgaa agccagacca caattcattt
     3061 tattcatcag ttgctaaagg atgaaactgt caatatcagt cagttagcac atggaatacc
     3121 atcaggtggt gaattagaat ttttagatgg gaatactata agcagtgccc ttaagaacag
     3181 agcagtgatt aatgtctaaa tccatattta ttttatgcag aaaaaaaata ctggtcttac
     3241 tcctgttatc cttatgtttc ataacaagta cagcttccgc ttccttccac agaagtttat
     3301 ggcccaaatg gctggtcaat gcccctcttt ccaaacagtc aatttctcat caggcatggc
     3361 aacgttttct tgatcatcat gtaataacta atgaagaaga catcaatctg gtagactata
     3421 ccaatatcaa tgagaaagag ctggcttcac taaaagaata tataaaaaat ttatcgcaaa
     3481 ttgatattga taattataac cgtcaagaac aattggctta ttggatcaat ttgtataatg
     3541 cccttacagt attaacagta gccaactatt accccattgc aaacattcag gaaattaata
     3601 tttctcccgg attatttagc gtcggacctt ggggagccaa tattatcact atcaaaaata
     3661 ctaacttatc cctggatgat atcaataata gaattattcg cccaatttgg aatgacccaa
     3721 gaactcatta tgctttaaat aatgccacca taggagcacc taatctgagc aaacaagcat
     3781 accaaggatc attgctggag caacagctta acgacgcggc attcaaatac attaactctc
     3841 tcaggggagt tcatgtgatt gaaggtaaat taattgtttc taaattatat gactggtatg
     3901 aagaagattt tggtggtacc aaaaaatatg ttatcaaaca tttgctacaa ttcgcgaaag
     3961 aac
//
LOCUS       DAXLDT010000228         3941 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXLDT010000228 DAXLDT010000000
VERSION     DAXLDT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.229"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(145..1380)
                     /locus_tag="ACF2SP_002922"
     CDS             complement(145..1380)
                     /locus_tag="ACF2SP_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFD2360683.1"
                     /translation="MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGV
                     AAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRF
                     ADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIEQLNIPVF
                     HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNML
                     LLDTKGVIHSGREDLNPYKFAFARNTSCRTLEDALVDADVFIGVARPDLLNAHLLSLM
                     APNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVR
                     ATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERV
                     PAAVAKAAIASGASQFNNL"
     gene            complement(1507..1734)
                     /gene="rpmE"
                     /locus_tag="ACF2SP_002923"
     CDS             complement(1507..1734)
                     /gene="rpmE"
                     /locus_tag="ACF2SP_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFD2360684.1"
                     /translation="MKASVHPDYQTVKVTCSCGEVFETRSTLCKDLNIEVCSMCHPFY
                     TGKQKLVDTGGRVQKFRDRYNMRTGQAKSKE"
     gene            complement(1871..2641)
                     /gene="xth"
                     /locus_tag="ACF2SP_002924"
     CDS             complement(1871..2641)
                     /gene="xth"
                     /locus_tag="ACF2SP_002924"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFD2360685.1"
                     /translation="MFKLASWNVNSLKVRLEQVLQWLESSQVDLLAMQETKLTDENFP
                     VASFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLI
                     NLYVPNGGELTSDKYQYKLDWLNKVTRFIENQLSIYSKVAVVGDFNIAPEDRDVHDPV
                     EWEGCVLVSPAERQAFMKILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDH
                     VLLSHELNALCRQSKIDKEPRKAERPSDHAPVWVELEL"
     gene            complement(2634..3416)
                     /locus_tag="ACF2SP_002925"
     CDS             complement(2634..3416)
                     /locus_tag="ACF2SP_002925"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFD2360686.1"
                     /translation="MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLI
                     PEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPK
                     LSVISLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
                     NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGW
                     RIDYQVITPGLTEYVADSRIFREARFSDHAPLMIEYKGDWCV"
     gene            complement(3472..3822)
                     /locus_tag="ACF2SP_002926"
     CDS             complement(3472..3822)
                     /locus_tag="ACF2SP_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFD2360687.1"
                     /translation="MVPADAKLLKQAKSLRKRSTDAENCLWYYLRAHRLKGYKFKRQV
                     PIGNYIVDFVCIQRKLIVELDGGQHLLNKEYDKDRSAYFSTLSYKVLRFWNDDVLLRT
                     GDVLESIVRCLEES"
BASE COUNT         1220 a          930 c          631 g         1160 t
ORIGIN      
        1 catatcttat aagcgcataa aagctcatct tgcaatctct ctgcaaacca gctggtggct
       61 ttacaaagaa tttttccaag atatccttcg tttaaacgct attaagacct aaactgctta
      121 cttctacaag gtgaatttat taccttataa attattaaat tgactcgcgc cactcgctat
      181 tgcagctttc gctacagcag caggaaccct ctcttttaag cgaggatcga taggctttgg
      241 aataatatag tcaggtccaa aatcccaatt cacaactcct gggtaattgt ccttcactac
      301 ctgaggaaca ggctcatgca ccaattgacg aatggcttca acagctgcaa tttgcatggc
      361 ctggttaatg caagtggcac gaacatctag cgcaccacga aaaatataag gaaaacataa
      421 tacgttatta acttgattag gataatcact tcgtcctgtt gccatcacca gatctttacg
      481 tacactatga gccaattcgg gtttgatttc aggatcaggg ttagataaag caaaaatcac
      541 tggattgggc gccatcaaac ttaataaatg cgcattcagc aaatctggcc ttgcaacgcc
      601 aataaacaca tcggcatcga ccaaagcatc ttcaagtgta cgacaggatg tatttctggc
      661 aaaagcaaac ttataaggat ttaaatcctc gcggcctgaa tgaataactc ccttcgtgtc
      721 cagaagaagc atattacttt ttggggcccc taatgccaca aggagtcgca tagaagcaat
      781 accagcagct ccagcaccca tgcagacaat tttaatgtca cttaatttct tgttctgtag
      841 ttccaaagca tttaacaaac cagctgcaac tacaatcgca gtaccatgct gatcatcatg
      901 gaatacagga atatttaact gctcaatcaa agcttgttct atttcaaaac actccggggc
      961 tttgatgtct tcaagattta tgccgccaaa agttggggca atgcgttttg cagtagcaat
     1021 aaaagactga ggatcctctg catcgatttc tatatcaaag acatcaagat cggcaaatcg
     1081 tttaaataat accgctttgc cttccatcac aggcttactc gctaaaggcc caagatttcc
     1141 caaacctaac actgcagtgc cattggtcat caccgcaacc agattgcctt tgctggtaaa
     1201 acgataagca ttttcgggcg cttcagctat ggctaaaact ggagcagcaa caccaggtgt
     1261 ataggccaaa gacaaatcgt cttgggaatt agtcgatttt gtaagatgaa cagagagttt
     1321 ccctggaaca gggaactcat gataatccaa tgcccgctgc tttattactt cattatccaa
     1381 agtgcatact ctctatttca gatcacgcgg tgaaaacatc atatcaaaaa tttgaatttt
     1441 taatcctctc ttagagaaaa ggcacaaaaa atttgtgcct ttgttaaaaa ctcaaaaatt
     1501 aaaatattat tcttttgatt tggcttgccc agtacgcata ttatacctgt cacggaattt
     1561 ctgaacacgg ccaccagtat caaccagctt ttgttttcct gtataaaaag gatggcacat
     1621 tgaacaaact tctatgttca aatctttaca caaagtagaa cgagtttcaa atacttcacc
     1681 acagctgcat gttactttaa ctgtttgata atcaggatga actgatgctt tcataatacc
     1741 actctcttct agtttcaaat aagtatcgcc acctggacac tattgccaag caccatactc
     1801 tacacaaatg agtcaaaaca atagcagaaa tgatctcata aagcaatttt ttctcaaaca
     1861 cctcagacat ttataattct aactcaaccc aaaccggcgc atgatcggaa ggcctctctg
     1921 ctttccttgg ttctttatcg attttggatt gtcggcataa agcgtttaat tcatggctca
     1981 atagcacatg atcaatgcgc aagccacgat tacgacggaa tgctgcggct ctataatccc
     2041 accagctaaa cgctttttct tcctgagcaa aattacggaa actgtcatgt aaacccaatt
     2101 caagtatctt catgaatgct tgcctttcag caggactgac caaaacacaa ccctcccact
     2161 caactggatc atggacatcc ctatcttcgg gcgcaatatt aaaatcaccc acaactgcta
     2221 ctttagaata aatgcttaac tgattttcaa tgaaacgagt tactttgttt aaccaatcca
     2281 gtttatattg gtacttgtct gaagtcagtt ctccaccatt gggcacataa agattaatta
     2341 atctgattcc ttttaccgta gcagccagga tacgccgctg gggatcttcc aattctggaa
     2401 tatcggtttg aatatctgtc ataggatatc gactaataat ggccaccccg ttataagttt
     2461 tctgacctga aaaaatgacg tggtaccctt tgtctgtaaa tgaagcaaca ggaaagttct
     2521 catcagtcag tttagtctcc tgcatggcaa gcagatcaac ttgtgaagac tcaagccatt
     2581 gcaaaacctg ttccagacgg acttttaatg aattaacatt ccagctagcg agtttaaaca
     2641 caccagtctc ccttgtattc aatcattaaa ggggcatgat ccgaaaaacg tgcttcgcga
     2701 aaaattcggc tgtccgcgac atattcagtc agaccaggcg taatcacctg ataatcaatc
     2761 cgccaaccga cattcttttc ccaggctcta gctcgataag accaccaggt atattgctct
     2821 tcttcctgat tatgtaccct aaaggcatct acaaacccca tagaaccaaa taactcatcc
     2881 atccaggcac gttcttcggg taaaaatccc gagtttttct ggtttcctcg ccaatttttt
     2941 aaatctatct ttttatgcgc gatgttgtaa tcaccacaaa taattaactc tcttccctca
     3001 ttttttaaat tcatcaagtg tctggcaaat tgctccagaa aagcatattt cacttcttgt
     3061 cgatcctcac cacttgttcc agaaggtaaa taaagagaaa tcacacttaa tttagggtaa
     3121 tcaaattgga tataacgccc ttctttatca cagtaatcaa accccaaccc cttcacaata
     3181 cgcacgggtt tatattttga ataaatggca accccactgt agcccttctt ttcagcatca
     3241 aaataatcac aaaaataatc ccttggaaaa taaagttctt ccgggattaa ttgctctact
     3301 tgcgatttcg tttcctgaat acatacaaaa tcaacatcct gccctacaag ccaatcataa
     3361 aatccattac gggcagaaga gcgtatgcca ttggcgttaa aactaattac tttcattact
     3421 taggcaactc cgttgtcatt actgaacggt taaatattaa tcctaccaaa tttatgattc
     3481 ttctaaacat ctgacaatac tctcaagtac gtctccagtt ctcaataaca cgtcatcatt
     3541 ccaaaacctc aaaaccttat aacttaatgt gctaaaatag gcgctccggt ctttatcata
     3601 ctctttattt aaaagatgct gcccaccatc caattcaacg atgagttttc tctgaataca
     3661 aacaaaatca acaatataat ttccaatagg aacttgtctt ttaaatttgt atccttttaa
     3721 tcgatgtgct cttagatagt accataaaca attttcagca tcagtgcttc ttttacggag
     3781 agattttgct tgtttcaata atttagcatc agctggaacc acttattccc ctctccccac
     3841 ccctctcccg cgaatcatac ccaaattttt tatacaattt ctttcgcggg agagggagtt
     3901 tttgtttaaa tatcagcgcc aattactaca attttcttta a
//
LOCUS       DAXLDT010000229         3940 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXLDT010000229 DAXLDT010000000
VERSION     DAXLDT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.230"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            21..2081
                     /locus_tag="ACF2SP_002927"
     CDS             21..2081
                     /locus_tag="ACF2SP_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360688.1"
                     /translation="MKFFLKQSHQHSSNQKKRNQPAQSEDHHLIIRQAQFGTITIDFV
                     GSIRLIFTFSIFCFRAIFGLLTGVVSKKELFDFVFNRTKIGLKSSTDTITVRRRATNE
                     TEKIQETTPKKSLLEKESEEPYISTNTKPTIKPNTTHTQNAFFTKSEEKSKTQSFKTV
                     EFHDFNNSEELVAFMEKINGLIAQDEAMAIPIKEDENLSSAGEEYIEKLIEIMKSSEL
                     LKHEFWNKLNEMVHKDIQLFVSKLSPAKEKGKFGSSDLDYLWRYFNGFEFEPSKRENR
                     KDKFAVMLEALSEEQLKASFDSPDFLSLLNKDYYIETAANTLKRKQLALFAANINRHD
                     ILNLMIKKLKPSAYLLGKLASVMPYATTKIKIALIDQIAHLPHSASFKIELAKLAEHY
                     ISMNTQARIKRYQSLPALPSNTIHTKPPLSKWSSVTAIPKPTYTNISVAKEEWTDNAF
                     EAFLSELNSTTYIINEKKIIEDLNSLPDHRIKMIAERASLPDYKDMLKHFWMIESKTI
                     NHRSFIENRKNRLKIILENLSESQLKNSIEDNKFWDIFRNTQEPYCKMAARALSPKQF
                     EIIISNATLERHSDFAVIISQIKKDSSADKERLQKIIEAIIPHTTPWFALALERQIKG
                     LLTSDKSLFEKFNTDLKKYLSKSLERPEVSLKYRRDRDLNRHAISHLLTEPLENSYKA
                     SFSL"
     gene            2322..3203
                     /locus_tag="ACF2SP_002928"
     CDS             2322..3203
                     /locus_tag="ACF2SP_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2360689.1"
                     /translation="MLKRVLNTAKAKQPISTTGIFAHTYYWLTSPAGDQSYQNPNYKN
                     EEDNNTGTAIYFIHGTADQPAAFKRVAERLIDAGLPDEICSLNLLAFDQRYQGKSIKF
                     FAEQLKNKIQANQHQRVILMAHSRGGLVASYFAEFLAKEASIEVPLVITVGTPFNGSY
                     LAVKPLSWFSDSIREMEIDSEFLAQLKQEIVEHSVSAYHFFIAKEDAIVPGESGYIKD
                     YVDKYPDSLHILDRHGHLSIMSSHRLVSRIAGLLHDYMDHCETHAEEVKISKLGELTL
                     IEDYYSVKSEPAPSFNP"
     gene            3390..3809
                     /locus_tag="ACF2SP_002929"
     CDS             3390..3809
                     /locus_tag="ACF2SP_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFD2360690.1"
                     /translation="MNFSAWYFPSLAALILYGAWGYWGTRASDFINPLSITFYSSIGV
                     LISGIIALILLGFKPELSVKGSTYGLLNGLANGIACIFFILALRNGPTMPVVLVTSMY
                     PMITLIFCMIFLKQELSLKQGLGMVFALLALVLFSTE"
BASE COUNT         1362 a          738 c          645 g         1195 t
ORIGIN      
        1 tatggatatt agttaatata atgaaatttt ttttaaaaca atctcaccag cattcctcta
       61 atcaaaaaaa aagaaaccag cctgctcaat ctgaagacca ccatttaata attcgtcaag
      121 ctcaatttgg cactatcact attgactttg ttggcagcat aaggcttata ttcacctttt
      181 cgatattttg ctttagagca atttttggtt tgcttactgg cgttgtttct aaaaaagagc
      241 tttttgattt tgtatttaat agaacaaaaa ttggccttaa aagttcgact gataccatta
      301 cagtgagaag aagagcaaca aacgaaactg agaagataca agaaacaacg ccaaaaaaat
      361 cattattaga aaaagagtca gaagaaccat atatttcgac caacactaag ccaactatca
      421 agcccaatac tactcatacg cagaatgctt ttttcacaaa atctgaagag aaatcaaaaa
      481 cacaatcctt taaaacggtc gaatttcatg atttcaacaa ttctgaagag cttgtggctt
      541 ttatggaaaa aataaatggg cttatagcac aagacgaggc aatggctatc ccgataaaag
      601 aggatgagaa cctgtcttcg gcaggggaag agtatattga aaaattaatt gagataatga
      661 aaagctctga gttactaaaa catgagtttt ggaataagct gaacgagatg gttcataaag
      721 acattcaatt atttgtcagt aaacttagcc cagctaaaga aaaagggaaa tttggctcaa
      781 gtgacctaga ttatttatgg cgttatttta atggttttga atttgagcct tcaaaaagag
      841 aaaatagaaa agataagttt gcagtgatgc ttgaagccct ttcagaagaa cagttaaaag
      901 cgtcttttga ttcacctgat tttttaagtc ttttaaataa agactactac attgagactg
      961 cagctaacac attaaaacgt aaacaattgg cattgtttgc tgcaaatatc aacaggcatg
     1021 acattctgaa tttaatgata aaaaaactaa aaccaagcgc ttatttgtta ggcaaacttg
     1081 cttctgttat gccttatgcc actacaaaaa ttaaaatagc tcttatcgat cagattgccc
     1141 atttacctca ttccgcttct ttcaagattg aactcgcgaa attggctgaa cattatatta
     1201 gtatgaatac ccaagcacgt ataaaacgtt accagtccct acccgctctc ccatccaata
     1261 caatccacac taaacctcct ctctcaaaat ggtcttctgt aactgctatc cccaaaccaa
     1321 catataccaa tatatctgta gcaaaagaag agtggactga taatgcgttt gaagcattcc
     1381 tgagtgaact gaattctaca acgtacatta tcaatgagaa aaagataatt gaagatttaa
     1441 atagtttgcc agatcataga attaaaatga tagcagaaag agcttcttta cccgattata
     1501 aggatatgct aaaacacttt tggatgattg aatccaagac aataaatcat agatctttca
     1561 tagagaacag aaaaaatcgc ctgaaaatta ttctagaaaa tttatcggaa tctcaattaa
     1621 aaaactccat agaagacaac aagttttggg acatttttag aaacactcaa gagccttatt
     1681 gcaaaatggc tgccagagca ttatccccca aacaatttga aataatcatt tctaatgcca
     1741 cactcgaaag gcattctgat tttgcagtaa tcatttctca aattaaaaaa gacagcagcg
     1801 ctgacaaaga aagactgcaa aaaattatag aagcaatcat tccccacact actccatggt
     1861 ttgctttagc tttagaacgc caaataaaag gtttattgac aagtgataaa tccttgttcg
     1921 agaaatttaa cactgattta aaaaaatacc tcagtaaatc gctggaaaga cccgaagtta
     1981 gtttaaaata cagaagagac agagatctaa atcgacatgc tatttctcat ctcctgacag
     2041 aaccacttga aaactcttac aaagcgtcct ttagtcttta aagatactct actactacct
     2101 taagaactct ctgcttttga ccggtaaggc accaattata aaaaccaata gagacgttaa
     2161 ccaaatcaca attcattcat aatccattga caataactga ccccgataac gctttaaagc
     2221 acagtttata attgactcat attaagctat attattgaga aattattgac agtatccgac
     2281 ttccggaact cgacgctcca caataaaaaa ggaatttaac tatgttgaag cgcgtactca
     2341 atactgcaaa agccaaacaa cccatttcca ccacgggtat atttgctcat acttattatt
     2401 ggctaacttc tcctgctggc gatcaatcct atcaaaatcc taattacaaa aacgaagaag
     2461 acaacaacac agggacggca atctatttta tccatggcac agctgatcaa ccagcagcat
     2521 ttaagcgtgt tgctgaacgt ttgatcgatg ccggtcttcc tgatgaaatt tgctctctga
     2581 atttactagc ctttgatcag cgttaccagg ggaaaagtat caaatttttt gctgaacaac
     2641 tcaaaaataa aatacaagct aatcagcatc agcgagtcat tttaatggcg cactccagag
     2701 gaggactagt tgcttcttat tttgcagagt ttttagcaaa agaagcctcc attgaggtac
     2761 cgttagtaat tacagtgggc accccgttta atggttctta ccttgctgta aaaccattgt
     2821 catggttttc tgattctatt agagaaatgg agattgatag tgaatttctt gcacaactta
     2881 aacaagagat agtagaacac tccgttagtg cgtaccattt ttttattgct aaagaagatg
     2941 cgattgtacc tggagaatca gggtatataa aagactacgt tgataaatac ccagattcgc
     3001 tgcatatttt agatcgccac ggccaccttt ccataatgtc ctctcatcgt ttggtatccc
     3061 gtattgccgg tttactccac gattatatgg atcactgtga aactcatgca gaagaagtaa
     3121 aaatttctaa attaggggaa ctcacactaa ttgaagacta ttattctgta aaatctgaac
     3181 cagccccctc tttcaatcca tgaaacgtcc attcatggat tttttggtta ttatttattc
     3241 agaaaaataa actagattgg taattagcgt ttttataaac cacagctcat catgataata
     3301 agtaaaattt agtaataaac agtattattt tcactaatga acagttatcc tgagcaaacc
     3361 tcaaacaaat tgcttgataa gataaaatta tgaattttag cgcttggtac ttcccttctt
     3421 tggctgcatt aattctgtat ggggcttggg ggtattgggg aacaagagcc tctgatttta
     3481 ttaatcccct atccattacc ttttattcaa gtataggagt tttaatctca ggaattattg
     3541 ctttgatttt attgggtttc aaacccgaac tttctgttaa agggagtact tatggcttgc
     3601 tcaatggact ggctaatgga attgcctgta ttttttttat tttggcatta cgtaatggcc
     3661 ccaccatgcc agtagttttg gtcacctcta tgtatcccat gattacttta atattctgca
     3721 tgatttttct caaacaagaa ttaagtttaa aacaaggatt ggggatggtt ttcgcactgc
     3781 ttgccttagt tttattttca actgaatgaa aactctaatc agatgaaata gctctaaatt
     3841 tctttggatt atcaaaatta aagagccttg ctctaaaaat taaaaatttt ggtcacgaag
     3901 cttgactctc aaattggtag tttgttcaaa aaatcttgaa
//
LOCUS       DAXLDT010000230         3937 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXLDT010000230 DAXLDT010000000
VERSION     DAXLDT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.231"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..336
                     /locus_tag="ACF2SP_002930"
     CDS             <1..336
                     /locus_tag="ACF2SP_002930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFD2360691.1"
                     /translation="ADLLKKIFRRSNDILFRLGGDEFAIIIANQPIKETISICETIKN
                     PFKTENLNHTYDSNEQLFMSHVTLSIGIVYIHFESSASIEHAITAADKALYQAKKKGK
                     NQIVVKKLL"
     gene            complement(398..1039)
                     /locus_tag="ACF2SP_002931"
     CDS             complement(398..1039)
                     /locus_tag="ACF2SP_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360692.1"
                     /translation="MKMKLQLIAIGILLSINVFADGYYPEPTSQDQPWTIIASFGSGR
                     YQHMYSKDGKTALGRLALANELMLSGDIALGLELGVQNGNKMRLNIPRETLSVLGWLP
                     VETNLSPMLDLLVTAKSDPLFGSSFFAQLKGGVAYRSWQVDLYPINEISQLAGEIQAG
                     FGYPITALASLSLLYQGVFGNDPNVKLNMSSKTGSVSNIPVLHGILLGFTVNL"
     gene            complement(1212..1299)
                     /locus_tag="ACF2SP_002932"
     tRNA            complement(1212..1299)
                     /locus_tag="ACF2SP_002932"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Ser,seq:gga)
     gene            1522..1956
                     /locus_tag="ACF2SP_002933"
     CDS             1522..1956
                     /locus_tag="ACF2SP_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360693.1"
                     /translation="MTLQRSIQKGVSDFSFIIGTGLKYGGAGGLLVMAVVGTVAIDLV
                     LLAAAEKHHNSFLTGFVLGSMFSRGNLDPVPLLIASPITSAVAVVLSVALGVPGVGLA
                     ILAGWTLAGTLLAVGMGLEAFAKSIDPEPSSESDYNSCLCFN"
     gene            2080..2526
                     /locus_tag="ACF2SP_002934"
     CDS             2080..2526
                     /locus_tag="ACF2SP_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360694.1"
                     /translation="MYLRLLALSALCFVTSPIWSFTCIYTLVKDNCWTDYDVTVDVIE
                     DSTSKTLLTLTAPKGKSWARGTFNCEAAEGLRYVAQFSPVFWQNDVGKTYPALRNWYL
                     PAKVNPGDLAWTIPVCFPADFAQVPFPPNVAGNCKCNFKNIPDPKL"
     gene            2653..3729
                     /locus_tag="ACF2SP_002935"
     CDS             2653..3729
                     /locus_tag="ACF2SP_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFD2360695.1"
                     /translation="MITIRNKIGQMLIMGFHGSELHDNSPVAQWLSNDGLGGVLLFDK
                     DLSTGIYGKNLRNQAQIKQLIRQLNRYASMISGGNDNLPLFTAIDYEGGVVDRLSKIE
                     GCPPTMKARELAKLSSDDFDEEVTQMAMTLQSLGFNLNFAPVVDLNLNAHAGIIGNLG
                     RSFSSNPEVTIRLAKQFVDVFSRYGIACAYKHFPGHGSATGDTHEGFVDVTETFQTDE
                     LIPYRSLLKDLSNPFMVMTAHVINKHLDSKGLPATLSYDVLTLLLRQSIGYDGVIISD
                     DLQMHAISNHYSLEDALCLTINAGADMVIFANQLGTITPPEVIDVIEKLVIDKQIPYQ
                     RIDEAYRRIVRLKQQIICSELINT"
BASE COUNT         1158 a          698 c          818 g         1263 t
ORIGIN      
        1 gctgatttat taaaaaaaat atttcgacgt tcaaacgata tactgtttcg gcttggcgga
       61 gacgaatttg caataatcat cgctaatcag ccgattaaag aaacaatatc tatttgtgaa
      121 acaattaaaa atccttttaa aacagaaaac ttgaatcata cttatgactc aaacgagcaa
      181 ttattcatga gtcatgtcac tttaagcata gggattgttt atattcattt tgaaagttca
      241 gccagtatag agcatgcaat tactgcggca gataaggcat tatatcaagc taaaaaaaaa
      301 ggaaaaaacc aaattgtagt aaaaaagcta ctttaagaag atgacattct atctttaact
      361 gagatggcgc ctggaagctg cgtcctagtt taatggatta taagttgaca gtaaatccta
      421 ataaaattcc atgtaaaaca gggatatttg aaacactgcc agtttttgag ctcatgttta
      481 atttgacgtt aggatcatta ccaaaaaccc cctgatacag caaactcaaa ctggccaaag
      541 cggtgatagg ataaccaaag ccagcttgaa tttctccggc taattgtgaa atttcattga
      601 tagggtagag atcaacttgc caagacctgt aagcgacacc accttttaat tgtgcgaaaa
      661 aagaacttcc aaataaaggg tcgcttttgg cggtaactaa taaatccagc ataggactga
      721 ggttggtttc taccggtagc catccaagga ctgataaggt ttctcgtggg atattcaagc
      781 gcattttatt gccgttttgg actccaagtt ctaatcccag agcaatatct ccactcagca
      841 ttaattcatt agccagagcc aatcggccta aagcggtttt gccatctttt gaatacatgt
      901 gctggtatct gccacttcca aaactggcaa taattgtcca aggttggtct tgagaggtgg
      961 gctcaggata gtaaccatca gcaaatacgt tgattgataa taaaattcct attgcaatta
     1021 gttgtaattt cattttcatc tttcatatcc ctgattgaat tggttggttc tgaaagcagt
     1081 tttaccaaaa gaatcttata tgagccagct gtagtcttta cacaatggat ataatttact
     1141 gctttatgca attcaatgaa agcggcaatt atagccttat accctgtgaa ggataaccag
     1201 gaattgtaat ttggcggaga gagagggatt cgaaccctcg atacgttgcc gtatacacac
     1261 tttccaggcg tgcgccttcg accgctcggc catctctcca accgggcaag cttatacttg
     1321 tcggcaaata aaagcaagac tgtgataatt attaaagagg ttttccatca caaagataat
     1381 atattctatt tttgtatagg aataaattgt atcgctttat ttgagtttta atccaatcta
     1441 ctcgttactt cggtgttcaa ttagtgttga ttatgtgcaa aattaagtgt attattcctc
     1501 aattttttag gagtaagatt aatgacactg caacgttcca tacaaaaagg ggtatctgat
     1561 tttagtttta ttattggcac aggtctaaag tatggtggtg ctggcggtct tttagtgatg
     1621 gctgttgtag gaacggttgc tattgatctt gtcttgcttg ctgctgcaga aaaacatcat
     1681 aacagttttt tgacaggatt tgtgttaggt tcaatgtttt ccagaggtaa tttggatccg
     1741 gttccacttt tgatcgcatc ccccatcaca tcagctgttg ctgttgtgtt atctgttgcc
     1801 ttgggcgttc ccggggtcgg tcttgcgata ctggctggtt ggactttggc tggcacttta
     1861 ttggctgttg gtatgggctt ggaagcgttt gctaaatcca ttgatcctga accttcatct
     1921 gagtcagatt acaattcttg tttgtgcttt aactaataga gccaagaggg gagattcaga
     1981 ttatttcccc ctcttaaaac agtttatcgg aaaattattt ttataaatat tgtatatact
     2041 atatagtaat aatggttcat taaatcttag gattgaatca tgtacttacg tttactcgcc
     2101 ctatctgcat tgtgcttcgt gactagccca atttggtctt tcacatgcat ctatactttg
     2161 gttaaagaca attgttggac tgattatgat gttactgtcg atgtcattga agattctacg
     2221 tcaaaaactt tgttgacact taccgctccc aaaggaaaat catgggctag aggtactttc
     2281 aattgtgagg ctgctgaagg gttgagatat gtcgctcaat tttcgcctgt cttttggcaa
     2341 aatgatgttg gaaaaactta cccggcatta agaaattggt atttaccagc aaaagtgaat
     2401 cctggagatt tggcctggac tatcccggtt tgttttccgg cagattttgc tcaagttccc
     2461 tttccaccta atgtagcagg aaactgtaag tgcaacttca agaacattcc tgatcccaag
     2521 ctttaatttt atgagaagca tttttttatt tgtacacatt gcgatactgt ttatagataa
     2581 tgcgtagcgg gcatagccag agagaatatc tttgtgtatg attttatgct tattttgtga
     2641 tggagcgagt gattgattac aataagaaac aaaatcggac aaatgctgat tatgggattt
     2701 catggttctg agctgcatga taatagtccg gttgctcaat ggttatctaa tgatggtctc
     2761 ggcggagtct tgttattcga caaagattta tccacaggga tttatggcaa gaatttaagg
     2821 aatcaggctc agataaagca attaattcga cagcttaatc gttatgcatc aatgatttca
     2881 ggaggtaatg acaatctgcc tttatttact gcgattgatt acgaaggtgg agtggttgac
     2941 agactaagca agatagaagg ctgtccgcct actatgaagg ccagggaact tgcaaagtta
     3001 tccagtgatg attttgatga agaagtaact caaatggcta tgacgttgca gtcattaggt
     3061 tttaatttaa attttgcccc ggttgtagat ttaaatttaa atgctcatgc agggattatt
     3121 ggaaacttgg gaagaagctt ttcttctaac ccagaggtta ctattcgttt ggccaagcag
     3181 ttcgttgatg ttttcagtcg ttatggtatt gcttgtgcgt ataaacattt ccccggccat
     3241 ggcagcgcca ctggagatac acatgaggga tttgtagatg ttacagaaac ctttcaaacc
     3301 gatgagctaa ttccttatcg aagtttatta aaagacctgt ctaacccttt catggtaatg
     3361 acggcgcatg tgattaataa gcatttggat agtaagggat tgcctgcaac tctgtcctac
     3421 gacgttttaa cgttattgtt acgtcaaagc ataggatatg acggggtcat tatcagcgat
     3481 gatttgcaaa tgcatgccat ttcaaatcac tattcattag aagatgctct ttgtcttacc
     3541 atcaatgccg gggctgatat ggtcatattt gccaatcagc ttggaactat taccccaccg
     3601 gaagttatag atgtgattga aaaattggta attgacaagc aaattcccta tcaacgtatt
     3661 gatgaggcgt acagaagaat agtacgtctg aagcaacaaa ttatatgcag tgaattaatc
     3721 aatacgtaat gcccatgcaa gtggtaactg gcgggtttat gaaacattaa gttaccactg
     3781 taactccgcc catttgagag gcgccatctt cttttacagc atgaacagat acaattgttt
     3841 gagatgcggt ttgaagtatt taattagtat ctatatggtg tgttctgtga agaatataac
     3901 aggacttacg aaaaacccca atctctttgt taaaaaa
//
LOCUS       DAXLDT010000231         3898 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXLDT010000231 DAXLDT010000000
VERSION     DAXLDT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.232"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..918
                     /locus_tag="ACF2SP_002936"
     CDS             <1..918
                     /locus_tag="ACF2SP_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2360696.1"
                     /translation="MDKLNLNLLRALYALLTCRHLTLASKKIGISQSSMSIYLKQLRE
                     HFHDDLLVRSQGNIMQLTPLANSLIGKARQALSLIETLFNTIETFNPLISERSFSIGM
                     TDLMSILLTPSLIKRLEQEAPGVKLSIKHPSYLNTVEVFESGDIDLMIGMFERVPEGL
                     KQQKLFQDEAVIVGGSNHPGIQKGKICIEELFKYPLIQFSLRETPFYNYFDRYLRQLG
                     HDKRVSISLGQGIMPLFVLTDSHYLTLSIRKVAEKLSHLIPLSIASVPFEVDSFNCYQ
                     YWHQKDDADPGHRWLREMVLSIAKKSYLK"
     gene            complement(1101..2561)
                     /locus_tag="ACF2SP_002937"
     CDS             complement(1101..2561)
                     /locus_tag="ACF2SP_002937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HFD2360697.1"
                     /translation="MTMKHLISRIFISVSAFFILDTASAGIPLWTFTPQSATTLQVPV
                     NGSAIIQYTIKNQSSKSHRLVIVPVPGLSQTAPCQLAPKGATGDTCTLNLVVTGSALP
                     EVGISGGPSLCQANPNGSPNPNLCYQPSAANSLNLSVGPAISNISVTPATLLFSENSS
                     GTITVTNTVGSPVAANNVVATIPGGSNITVQSSTCPSSLAIGASCTITLASGIQEGPI
                     PISVAGSNTNTVITLVTVTSRPTIFISAPIQANRVIEVGVITPLVLTITNDVSSLVNA
                     NNITISNQTNCPDVTFDDSNCTSVTPGSNCNLELNSPTPYIPCTITISGSNTANSPTT
                     PIAFQFLNGLVFETNGVNGKVVSLLAAEFNDIEWTFTDANIAGTSDLNDGITNTNNIV
                     ADPTCSNNPLNCAANRCRTLGPVWYLPAINELQALAAILCPGGTCNFGGFANDAYWSS
                     TQAGINDAQGNSFPNVNTVLHPKNDQDRVRCIQAFP"
     gene            complement(3002..>3898)
                     /locus_tag="ACF2SP_002938"
     CDS             complement(3002..>3898)
                     /locus_tag="ACF2SP_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360698.1"
                     /translation="FSPLQDPWSELEELGFNPEYLKRLKERAATSIEMTKNIEMLADY
                     YEQFKEVGFTDRDLARIVSLVSFTADNRLANIGSRMMITLVCGGLNLVKAPLHFKPHK
                     LTNMMLSLNGLEKLRTLVEMSKESGFTPFKNQAEFKASFEKRCKKFPILRVLWCYSDA
                     NESYMEYRKQDAIYKDWLSEEELHQLNRLSRISKTRDLHYWKRDTVRPVLLGDNYYSR
                     EDFGQTREEFEKAQDNPDAVESWCDSWANPFDQNPDSALTQSQEKSNQRNASKIPFFT
                     VYENNGKSGVVDEQQSSNAFSN"
BASE COUNT         1127 a          762 c          776 g         1233 t
ORIGIN      
        1 atggataaac ttaatctcaa tttacttcgc gctctgtatg cactattaac ctgccgtcat
       61 ctaacacttg ctagcaaaaa aataggtata agccaatcct caatgagtat ttatttgaag
      121 caactgcgtg agcatttcca tgatgatttg cttgtacgta gccaaggcaa tattatgcaa
      181 ttaactcctt tagctaactc gttgatcggt aaagcccgac aagctctctc actgattgaa
      241 acactattta atactataga gacctttaac cccttaattt cagaaagaag tttctcaatt
      301 ggcatgactg atttaatgtc tattttgttg actccaagtt tgattaaacg attggaacaa
      361 gaggctccag gagtcaagct aagtatcaaa catcctagtt atttaaatac ggtagaagtg
      421 tttgagtccg gtgatattga tttaatgata ggtatgtttg agagagtccc agaaggctta
      481 aagcaacaaa aattgtttca ggatgaagcc gtcatcgttg ggggaagcaa ccatccagga
      541 atccaaaaag ggaaaatttg cattgaagaa ctatttaagt acccattaat tcaattttca
      601 ttaagagaaa ctccttttta caattatttc gatcgctatt tacgtcaact gggacatgac
      661 aaacgggttt ctattagctt aggacagggg atcatgcctt tatttgttct tactgactct
      721 cattatctta cattaagtat tcgaaaagta gctgaaaaac tttcccattt gataccatta
      781 agtattgcct ctgtaccctt tgaggttgac agttttaatt gctatcaata ttggcaccaa
      841 aaagatgatg ctgatcctgg ccatcgttgg ttacgggaaa tggttctcag tatagctaag
      901 aaatcttatc taaaataatc agttaaaacc cactctataa agcagctgtt gcagtgacta
      961 ataaaaatgc acgtcctatg tgggttatta tggctgacga gtccatatac gtagcccggg
     1021 acacgggcta cttgcttttt cgaaacctcc gagagtcaga tcctgaacga tggactgttg
     1081 aacgaagtaa cttccaagag ctaaggaaaa gcctggatgc agcgtactcg atcttgatca
     1141 ttttttgggt gcaaaacggt attaacgttt ggaaaagagt ttccttgtgc atcattaata
     1201 cctgcttgcg ttgagctcca ataggcatcg tttgcaaatc caccaaagtt acaggtgcct
     1261 cctgggcaca gaatggcagc tagagcctgt aactcattaa ttgcgggcaa gtaccaaact
     1321 ggcccaaggg ttcgacacct atttgctgca caatttaagg gattattgct gcaagttggg
     1381 tctgcaacaa tgttatttgt attagttatt ccatcattga gatctgaagt cccggcaata
     1441 ttagcatcgg taaacgtcca ttcaatgtca ttaaactccg cggcaagaag tgataccact
     1501 ttaccattaa caccattcgt ttcaaaaacc agaccattta ggaactgaaa tgcaattgga
     1561 gttgttggac tatttgccgt gttactgccg ctaatggtga tggtgcacgg aatatagggc
     1621 gttggggagt tcaattcaag gttacagttg ctgccaggag ttacactagt acaattgctg
     1681 tcatcaaacg ttacatcagg acagttggtt tgattactta ttgtaatgtt gttcgcattg
     1741 acaagacttg atacatcatt agtaattgtt aatacaagtg gggttataac ccctacttca
     1801 atcacccgat tagcctgtat tggcgcacta atgaaaattg taggccgact ggttactgta
     1861 accagagtaa tgactgtatt ggtattagaa ccagctacag aaatcggaat cgggccttcc
     1921 tgaatccctg aagcaagcgt tatggtacaa cttgcaccaa tagcaagact tgaaggacag
     1981 gtactgcttt gcactgtaat attgctacca ccaggaatag tagccacaac attattggca
     2041 gcaactggtg agccaactgt gttggttact gtgatggtcc cggagctatt ttctgaaaac
     2101 agcaaagtag caggtgtaac actgatatta ctaatagctg ggcctactga aagatttaaa
     2161 ctattcgcag cactaggttg ataacacaga tttggattag ggctgccatt gggattggct
     2221 tggcatagag agggtcctcc tgaaataccg acttcaggta aagcgctgcc cgttacgacg
     2281 agattaagag tgcaggtatc tcctgttgcc cctttgggtg ccagctgaca tggagctgtt
     2341 tggcttaatc ctggaacagg tacaatcact aacctgtgag atttcgaaga ttgattctta
     2401 atcgtatact ggattattgc ggagccattg actggtactt gaagtgtcgt tgctgattga
     2461 ggtgtaaatg tccataaagg aatgccagcc gatgctgtat ccagtatgaa aaaagcgctg
     2521 acgctaataa aaattcgact aattaaatgt ttcatcgtca tgtttttgta tatccttatt
     2581 ttcggcatta gatggccatc atgttgccag actaattctg cacttaccag agggttatag
     2641 ccaagatttt cttgcctgat agatatggca caaaattagg tacggcgctg tactcagagg
     2701 accaggtctt tcctttttct ttttcaggca aaagaaacga tctgaccgaa tgggctggag
     2761 aattaagtca tgttaagaat tctaaatttt tttaacatgc ccctttaatc cacaacagta
     2821 acattcatta ataatttata aaaactatcg ttcaagtgta aattgtcttt gttgtaaagt
     2881 ttgcaacact cttgtttatt ttcgttaaca tagttcagct atagtaacga tccttcgggt
     2941 caaaataact caaccttgct cagcaagatc cagtcttgcc aagctgcact tatttacttt
     3001 attaatttga aaaggcattt gaagattgtt gctcatcgac aacaccactt tttccattat
     3061 tttcataaac agtaaaaaaa ggtattttac tggcgtttct ctgattcgac ttttcttgtg
     3121 attgtgttaa agcagaatct ggattttgat caaaaggatt tgcccatgag tcacaccaag
     3181 attccactgc atctggattg tcttgtgctt tttcaaactc ttcccgtgtt tgtccaaaat
     3241 cttctctgga ataatagtta tcgccaagta aaactggtct tactgtatcg cgtttccaat
     3301 agtgaagatc tcgcgtctta gatattcgag ataatctgtt aagttgatga agttcctctt
     3361 cgcttagcca gtctttatag attgcatctt gttttctata ttccatgtag gattcatttg
     3421 catcagaata acaccataaa actctgagga ttggaaattt tttacatctt ttttcaaaac
     3481 tcgctttgaa ttctgcctgg tttttaaatg gtgtgaaccc actttctttg ctcatttcaa
     3541 ctaatgttcg taatttttcc aaaccattta aactaagcat catattagtc agtttgtgcg
     3601 gtttgaagtg gagaggagct tttactaagt ttaatccccc gcagactaag gttatcatca
     3661 ttcggcttcc gatgtttgcc agacgattat ccgcagtgaa actgaccaaa ctcacaattc
     3721 ttgccagatc gcgatcagtg aaacctactt ctttaaattg ttcataatag tctgccaaca
     3781 tttctatatt tttagtcatc tctatagaag tagcggctcg ttctttaaga cgtttcaggt
     3841 attctgggtt aaaaccaagc tcttctaatt cggaccacgg atcttgtaat ggagaaaa
//
LOCUS       DAXLDT010000232         3850 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXLDT010000232 DAXLDT010000000
VERSION     DAXLDT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.233"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..658
                     /locus_tag="ACF2SP_002939"
     CDS             <1..658
                     /locus_tag="ACF2SP_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFD2360699.1"
                     /translation="KSFLKLIVTSNEIANFVLDNPKLRAKLDGIEPLVDAQKFENSCT
                     ARAIMKILLERGLIEPSSYTPGKELEIYKDIWLEPGKVASPEKIASYFCKYNLDVIGV
                     EIRELSKSVRNKYSKDMVITSLYSLFKKEVPIRKKMTLTTLSEADFPEGITTLIIIKA
                     GVLHTLLGKKHHGQFEVTDPWFGDKKIYSGFMDFLEKERKNLGVFFEVSQGSQEIFRP
                     "
     gene            820..978
                     /locus_tag="ACF2SP_002940"
     CDS             820..978
                     /locus_tag="ACF2SP_002940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360700.1"
                     /translation="MPSRSLVWNLMFRAKASRRCYASPQPKRAVDIDCFLYNPYPEPT
                     SDKSETRH"
     gene            complement(1035..1799)
                     /locus_tag="ACF2SP_002941"
     CDS             complement(1035..1799)
                     /locus_tag="ACF2SP_002941"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360701.1"
                     /translation="MSNQNLAIIIGGSSGMGLETAKKLSSNNMELLITGKDKQKLENA
                     AAEIQKLGAKVDILVLDLYDKEQVFQFMSSMNDETRRVKYLVNAAGYFKPITFLEHEE
                     EDYDRQVNLNKAFFFITQAVVKNMKKNGGGSIVNIGSMWANQAVKATPSSAYSMQKAG
                     LHSLTQHLAMELAEHNIRVNAVAPAVVATPIYKSFIDEKRIEESLQDFNKFHPIGRIG
                     KATDIADAIEFLLSEKASWITGEIMNVDGGVMAGRN"
     gene            1894..2274
                     /locus_tag="ACF2SP_002942"
     CDS             1894..2274
                     /locus_tag="ACF2SP_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFD2360702.1"
                     /translation="MVQIIEMDEKKLIQKKAPIKTASMVEAIYGCKWSLTIYSLLRNG
                     INRPGEMVRSVEGLSTKVLNQCLRKNIEFGILGRNTFHEIPPRVEYFVTPFGEKFLKI
                     LDQIEELNEQMRDYQQIEFNNNDS"
     gene            2408..2995
                     /locus_tag="ACF2SP_002943"
     CDS             2408..2995
                     /locus_tag="ACF2SP_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HFD2360703.1"
                     /translation="MARNDPIALLTTWISEELERGAPNPQHATLSTTGKDSIPHGRVI
                     AIREINEQGLLFFTQRGTRKVVEMTYNPMVSLTFWFELFEREVMIDGSARPLSESENQ
                     TYWDTYPPFAQIRFHSYAPTSSQVIPSKQLIENKRKILTSQFQDKPLPMSEYYCGFRV
                     IPTRMVFYTYRTDELSDVVEHCFIKNNWEKNLLSP"
     gene            complement(3007..>3850)
                     /locus_tag="ACF2SP_002944"
     CDS             complement(3007..>3850)
                     /locus_tag="ACF2SP_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD2360704.1"
                     /translation="KAAEKSFMSQTAMSHILKQLRDIFDDPLFIRKPHGLQPTQKVLD
                     LAPKIKKFLNYSNDIFEEDTFNPGNDNLFLKTASVGTGEYFILPRLCAHLAKNAPKFV
                     LKVSSLSEYLSLEYLLMSELDFAIAPGFVETGNNIIRELLFEEEAVCVMHKSHPLANQ
                     ELTQDMYLQAEHVDIQFSYMGNENILYKALQGYHQRNIKVIVPNIISALEVVYHTNFI
                     TTVPRILAVTFKDRYQIELHPFPFPKKTFLVNFYYHKRLSNHKPLQWVLDIIKEYCCI
                     NPEA"
BASE COUNT         1235 a          701 c          706 g         1208 t
ORIGIN      
        1 aaaaagtttc ttaaaattaa tagtaacttc caatgaaatt gcaaattttg tattggataa
       61 tcctaaacta agagcaaaac tggatggcat tgaacctttg gtcgatgcac aaaaattcga
      121 aaattcttgt actgcaagag caataatgaa aatacttctt gaaagggggc ttattgaacc
      181 aagcagctat acacctggca aagaattgga aatatacaaa gacatttggc tagaacccgg
      241 taaagtggca tcgcctgaaa agatcgcatc ctatttttgc aaatataact tggatgtcat
      301 tggtgtcgaa attagagagc tatcaaaatc tgtacgaaat aaatattcaa aagatatggt
      361 tattacttca ctctactcac tttttaagaa agaggtgcca ataagaaaaa aaatgacatt
      421 gacaacctta agtgaggcag atttccctga ggggataacc actcttatta ttataaaagc
      481 tggagtactc cataccttat taggcaagaa acaccatggg cagtttgaag taactgatcc
      541 ctggtttggt gataaaaaaa tatatagtgg attcatggat tttcttgaaa aagagagaaa
      601 aaatctggga gtattttttg aagtttctca aggatcccaa gagattttta gaccgtaatg
      661 gttatcaatg tttgccaaaa gcaaatggcc acattattcc atgcaggcaa tgaaaagcct
      721 tagtgcgagc atcaatagca aaagattgat ttttatggat aacgtgagtt atggataagg
      781 cagccattat tttgaatcgc tcgggctgag gaggcattta tgccgtctcg aagccttgta
      841 tggaatttaa tgtttcgtgc caaggcttcg agacgatgct acgcatctcc tcagcccaaa
      901 cgggctgtgg atattgactg cttcttatat aatccttatc cagagcccac atcagataaa
      961 tctgaaacaa ggcattaaaa atcaatcttt tgctattgat gctcgtgggc ataaatctgg
     1021 ataaggtttg ctgattaatt tcttcctgcc attacgcctc catcgacatt cataatctcc
     1081 cctgtaatcc agcttgcttt ttctgacaaa agaaattcga ttgcatcagc gatatctgtt
     1141 gccttgccga ttctgccgat aggatgaaat ttattaaaat cctgtaacga ctcctcgatt
     1201 ctcttttcat caataaatga tttatagata ggagtggcta ccactgccgg tgccacggcg
     1261 tttactctta tattgtgctc agcaagctcc atagctaaat gttgggttag tgaatgaagt
     1321 ccggcttttt gcatggagta agcagaggag ggggttgctt taaccgcctg attcgcccac
     1381 atcgaaccga tattgacgat actaccccct ccattttttt tcatgttttt tactacggcc
     1441 tgggtgataa aaaagaacgc cttattaaga ttaacctgac gatcgtaatc ttcttcctca
     1501 tgttctaaaa aagtgatggg tttgaaataa cctgcggcat ttacaagata ttttactctt
     1561 cgagtttcat cattcatact actcataaat tggaatacct gctctttgtc ataaagatca
     1621 agaactaaaa tgtctacttt tgctcccagt ttttgtattt ctgccgcagc attttcaagt
     1681 ttttgtttat cttttccggt aattaacagc tccatattgt tacttgaaag tttttttgca
     1741 gtttcaaggc ccataccact agagccgcca ataataatag ctaaattttg attggacata
     1801 gattctcctg attcattaaa atactgtcca atccagtatg aaagaactac aatattaaaa
     1861 aaagtaagca cttttcagtt cactaagtac tttatggtac aaattattga aatggatgaa
     1921 aaaaaattaa tacaaaaaaa agctcccatt aagaccgcat caatggtaga ggctatctat
     1981 ggttgtaagt ggtccttgac tatctattct cttttacgaa atggaatcaa tcgaccaggt
     2041 gaaatggtac gaagcgttga aggactatca acaaaagttt taaaccaatg cctgcggaag
     2101 aatattgaat ttgggattct tggcagaaat acttttcatg agataccccc cagagttgag
     2161 tactttgtga ctccttttgg agaaaagttt ttaaaaattc tggatcaaat agaagagttg
     2221 aacgagcaaa tgagggatta tcaacaaatc gaattcaaca ataatgatag ttaagatttg
     2281 ttataaaaga cctattgcta atcgactgca agtgaattat ggctcacaat atttaccaat
     2341 gtaatgagac atcgctagtc cgtatcatta ttaacgccag aaaatattta caataggtat
     2401 tcaagttatg gccagaaacg accccattgc ccttctaaca acatggattt cagaggaatt
     2461 ggaaagagga gcacctaatc cacaacatgc cacattatct acgacaggca aagattctat
     2521 tccccatggg cgtgttatcg ccattcgcga aatcaatgag caaggtttat tattttttac
     2581 ccaacgagga accagaaagg ttgtcgaaat gacctataat cctatggtat ctctgacttt
     2641 ctggtttgag ttatttgaaa gagaagtcat gatagacggt tcagccaggc cattatcgga
     2701 aagcgaaaat caaacatact gggataccta tccgcctttc gcgcaaatca gatttcatag
     2761 ttatgcgcct acttcatcac aagtgatacc cagcaaacaa ctcattgaaa ataaaagaaa
     2821 aatattgact tcccagtttc aagacaagcc attgcctatg agtgaatatt attgtggttt
     2881 cagagtcatt cccacacgca tggtttttta tacttataga acggatgaat tgtctgatgt
     2941 cgtagaacat tgttttataa aaaacaattg ggagaagaat ctgctttctc cttaatatca
     3001 atttgtttaa gcctcaggat tgatacaaca atattctttt attatatcca atacccattg
     3061 cagcggttta tgatttgata atcgtttatg ataataaaaa ttaactaaaa atgtcttttt
     3121 agggaaaggg aaagggtgta gttcaatttg atatctgtct ttgaaagtaa cagctagaat
     3181 tctcggtaca gttgtaatga aatttgtatg gtaaaccact tccaatgcgc taatgatgtt
     3241 gggtactatc actttgatat ttctttgatg atatccttgt aacgctttat atagtatatt
     3301 ttcatttccc atgtaactaa attgtatgtc aacatgttca gcttgcaagt acatatcttg
     3361 agttaattcc tgatttgcca gaggatgaga tttatgcatg acgcacacgg cctcctcttc
     3421 aaacaataac tctctgatta tgttattccc tgtttcaaca aagcctggcg ctattgcaaa
     3481 gtctaattcg gacattaata aatactcgag actcaagtac tctgacagtg aagaaacctt
     3541 taatacaaat ttcggagcat ttttagccaa atgagcacat aacctgggta aaataaaata
     3601 ttctccagta cctactgatg cagtctttaa aaataaattg tcatttcctg gattgaaagt
     3661 atcttcttca aaaatatcat tggagtaatt taaaaatttt ttaatcttgg gggccagatc
     3721 aagtaccttt tgtgttggtt gaagcccgtg cggtttcctg ataaacaaag ggtcatcgaa
     3781 tatatcacgt aattgtttta atatatggct cattgcagtc tggctcatga aagatttctc
     3841 agctgctttg
//
LOCUS       DAXLDT010000233         3848 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXLDT010000233 DAXLDT010000000
VERSION     DAXLDT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.234"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..893
                     /locus_tag="ACF2SP_002945"
     CDS             <1..893
                     /locus_tag="ACF2SP_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2360705.1"
                     /translation="KNFFVGLWSSTLIIILASYLLAKKSMRPIKIFTKELASINASSL
                     EKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRNATEI
                     TLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRRLSANHLISS
                     IIEYYYSIAQEKSIEIQMFGEAHIEVDEHLFKRVISNLIDNSLTYTNKGGRIIIIIEK
                     RTDDSIQICIKDNGIGISKEHLPLLYHGFYRVDYSTKKENAGLGLGLAIAKSIMEHHK
                     GELVVDSKVGLGTTVILTLP"
     gene            complement(1086..>3848)
                     /locus_tag="ACF2SP_002946"
     CDS             complement(1086..>3848)
                     /locus_tag="ACF2SP_002946"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFD2360706.1"
                     /translation="QTLFNQGTISIIQDANLGAGGALTFNGGVLQTTADLTSNRTATL
                     NSGNGTFFTDAGTTLTYNGQITGIGQLRKNGDGTMILGSTLNNYSGGTLILDGTLQAT
                     TATLPGNVIVNSGAYLEFHQNFDGTYNGVISGAGDVIKDGSGNVTLTGIQTYSGQTTI
                     NQGGIQGTTTSINNQSVNTTAVNTALIFNQNFDGTYSGILSGLGSLLKFGTGTVTLTG
                     ANTYSGGTFIDAGAFSISSAANIGGSASPLVFFSGALITTATMILDNTTHLGLLGGTF
                     VTNPATTLTMNGQVMGVGSLTKEGSGTLILNNASNNYTGGTIVQEGILQAGVSGGLSN
                     HSHYTINGGQLDLNGYALSMGLLTGLGGTVDVTATSLILEQSLDSTYAGSFLGDTTAI
                     IEKNGSGQLTLTGNSSSYQGAMNIDQGTLVMNGQMNGHLNIGNSGTLKGVGAVNDLSV
                     YGTIAPGNSIGTLTVNGNYIQNPGSFYELEFNPSGNTDLISVLGTATINGGTVRLLPD
                     QPPYTPGTKYTILEAQGGVSGTYSDLTNPNLPYLFFDLSYDANHVYLEVFRSGIDFSA
                     LAITPNQIATANAIETIRPSNPLYIAIVNLDTAEEAQSAFNLLSGEIHASIIGSVVEE
                     SRYLRDAIFQHLTDENPNNNDGVWLRTYNAKTTRDTDNNAATLDGNSSGFFIGVDKKI
                     TSNVKIGSVFGYGTRQDSVPHRLSNANLQQFDFTLYSEYVHQQLYLRYGYGHFWYSAS
                     THRTVELPTLVNYLRSDYSLYSNQFFAEMGLKGLYKNTPVDPVAQVAFIEAGNNQFNE
                     RGGISALSGSANHMKTPITTLGFRSKTTLFNSDKLDVNSNGMLGWQHAYSDTVPGSIM
                     GFASSANFLIGGTPIAKDSALVNTGLEFKNPQYDSLKMNLFYQGVFADNVSNNSILVS
                     LNWGFN"
BASE COUNT         1197 a          776 c          687 g         1188 t
ORIGIN      
        1 ttaaaaattt ctttgttgga ctctggtctt ctactttgat aatcattctt gccagttatt
       61 tgctggcaaa aaaaagcatg agaccaatta aaatattcac taaagaactt gcatcgatta
      121 atgcttcatc actagagaaa cgattacctt atacagggaa tcccaaagaa ctggaagagt
      181 tagctttgac ttgtaatgat atgctcttta gaatagaaag tgcatttagc catataaagc
      241 agttttcagc gagtatagct catgaattac gaaatcccat tcattacctc cgaaatgcta
      301 ccgaaattac cctgactaaa ccacaaacga ttgaaaccta ccaacatgta ttacaaacgc
      361 accttgaaga atatcaaagt ttaacccaac ttatagataa tctgctcttt ttaactcgct
      421 gtgagcatgg ccaacttcaa ttaaatgtta gaagattatc tgcaaaccac ttaatcagtt
      481 caattatcga gtattattat tcaatagctc aagaaaagag catagaaatt cagatgtttg
      541 gtgaggctca cattgaagta gatgaacatt tatttaagcg cgttatttca aatttaatag
      601 acaacagtct gacctatacc aacaaaggtg gaagaataat aatcatcata gaaaaaagaa
      661 cagacgacag tattcaaatc tgtataaaag ataatgggat tggtatatca aaagaacacc
      721 tacctctttt atatcacgga ttttatcgcg tagattatag tactaaaaaa gaaaatgcag
      781 gtcttggttt aggtttggct atcgctaaat caatcatgga acaccataaa ggcgagcttg
      841 tagtagatag caaggtcggt ttaggcacta cggttatatt aaccctacca tgagaaaagc
      901 tttttaaaga ttgaataatg aatgtatttc atctatccgg gaccttaagg gaacggatat
      961 tgaactaagt gattctaaga gcatagactt ttcccgcctc acacagcaat actaatctac
     1021 aataagtccc agcgctcagg aacttgcgct cagatggaaa aacccaaagt tctatggcgt
     1081 gaatattaat taaaacccca gtttaatgaa actaaaatac tattatttga aacattatct
     1141 gcaaatacgc cttgataaaa tagattcatt tttaaagaat catattgcgg gtttttaaat
     1201 tctaatcctg tattcactaa tgcgctatcc ttcgcaattg gagtacctcc aattaaaaaa
     1261 ttagcacttg atgcaaaacc cattatgcta ccaggtactg tatcggaata ggcatgttgc
     1321 cacccgagca ttccattgct attaacgtcc agtttatctg aattgaatag agtagtctta
     1381 cttctaaaac ctaatgttgt aataggcgtt ttcatatgat tggctgaacc gcttagagca
     1441 cttattccac cacgttcatt aaactgatta ttacctgctt caataaaagc aacttgtgca
     1501 acaggatcta caggagtatt tttgtatagt cctttaagcc ccatttctgc aaagaattga
     1561 tttgagtaaa gtgaataatc agacctcaaa tagttaacta aggtgggtaa ttcgacagtt
     1621 ctatgtgtgg aagcagaata ccagaaatgt ccataaccat atcgaaggta caactgttga
     1681 tgaacatatt ctgaatataa tgtaaaatca aactgttgca aattggcatt tgaaagtcga
     1741 tggggtacac tatcctggcg agtaccataa ccaaagacac tacctatttt cacattagaa
     1801 gttattttct tatcaacacc tataaaaaac ccagaactgt ttccatctag tgttgcggcg
     1861 ttattatcgg tatcacgtgt tgttttagcg ttataagtcc ttaaccatac gccatcatta
     1921 ttattagggt tttcatccgt aagatgttga aatatggcgt ctcttaagta acggctttct
     1981 tcaactacag agcctataat tgatgcatga atttctcctg ataataaatt aaaagctgac
     2041 tgcgcttctt ctgctgtatc taaattaaca atggcaatat aaagtggatt tgatggccga
     2101 attgtttcga tagcattcgc tgttgcaatt tggttcggcg taattgccaa tgctgagaaa
     2161 tcgataccac ttctaaaaac ttccaaatag acatgattgg catcataact taaatcaaaa
     2221 aacaggtagg gtaaattagg attggtcaaa tctgaataag tccctgatac ccctccttga
     2281 gcttctaaaa tagtatactt ggttccaggt gtgtatggag gttggtccgg gagtaaccgc
     2341 actgttcctc cattaatggt tgccgttcct aaaacagaga ttaggtctgt attgcctgag
     2401 ggattaaact ctaattcata aaaagaacct gggttttgaa tataattccc attaacagtc
     2461 aatgttccaa ttgaattgcc tggcgcaatt gttccataca ccgataaatc gtttactgcc
     2521 cctactccct ttagcgtccc tgaatttcca atattaagat gcccgttcat ctgcccattc
     2581 atcactagcg ttccctgatc aatattcatg gctccttgat agcttgaact attgccagtt
     2641 aaagtgagct gccctgaacc gtttttttcg ataatcgccg ttgtatcacc aagaaaggag
     2701 ccagcatatg tgctatcaag gctttgctct aatattaatg atgtagccgt aacatcaacc
     2761 gtcccaccaa gacctgtaag aagccccatg ctgagtgcat atccatttaa atcaagctgg
     2821 ccaccattaa tcgtgtagtg cgaatgattg cttaaccctc cagaaacgcc tgcttgaagg
     2881 ataccctctt gtacaatagt tcctcctgta taattattcg atgcgttatt gagaatgagc
     2941 gttccggagc cttctttggt cagtgagcct acccccataa cctgcccatt catggttaag
     3001 gttgttgcag gattggtaac gaacgttcct cccaacaagc ccagatgggt agtattatct
     3061 aaaatcatgg tcgctgtagt aattagagca ccagaaaaaa atacaagtgg gcttgcacta
     3121 cccccgatat ttgcagcgga actaatacta aatgcaccag catcaataaa tgttcctcct
     3181 gaataagtat tggctcctgt taatgttacc gtacctgtgc caaattttag taatgagcca
     3241 agcccactta aaataccact gtatgttcca tcaaaattct gattaaaaat cagtgcggta
     3301 ttgacagctg ttgtattgac actttgatta ttgattgagg ttgtggttcc ctgaatacca
     3361 ccttgattaa tcgttgtttg accactgtag gtttgaattc ctgttaaggt gacattacca
     3421 cttccatctt ttatcacatc accggcaccg ctaataacac cattgtaggt accatcaaaa
     3481 ttttggtgga actctaaata agcaccgcta ttgacaatga cgtttccagg taaagttgca
     3541 gttgttgctt gaagcgtacc gtctaatatt aacgtgccac ctgaataatt gtttagtgtg
     3601 cttcctaaaa tcattgtgcc atcgccattt tttctaagtt gtccaattcc cgtaatttgt
     3661 ccgttatagg taagtgttgt tcctgcatcg gtaaagaagg ttccgtttcc actattaagt
     3721 gtcgctgttc tattcgaagt taaatcagct gtagtttgca gaacccctcc attaaacgtt
     3781 aatgctcctc ctgctcccaa attggcatct tggatgatgc tgattgtacc ttgattaaat
     3841 aaagtttg
//
LOCUS       DAXLDT010000234         3844 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXLDT010000234 DAXLDT010000000
VERSION     DAXLDT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.235"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(8..1180)
                     /locus_tag="ACF2SP_002947"
     CDS             complement(8..1180)
                     /locus_tag="ACF2SP_002947"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFD2360707.1"
                     /translation="MKAKVILVNGKQNTVLRGHPWIYPKAIAQYSGKLITGELVEIIG
                     SEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQ
                     TNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHSHVIWL
                     PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKL
                     SRGKRVLDLYTFTGGFALHAAKAGALQVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKA
                     DAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNC
                     SSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV"
     gene            1258..2241
                     /locus_tag="ACF2SP_002948"
     CDS             1258..2241
                     /locus_tag="ACF2SP_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360708.1"
                     /translation="MRQFTKYLAIFLFSSSVSVAYAGPWFTGPLLAPAGHTVPKGHTN
                     LEVYGIDVFTDGQYNASGQRISTPKFKTFAANPILTHGFTDWLDVQLTVPYLFNSTRG
                     VHENHLADVATALGIQILEQKSSPKRIDFRVLLQETIPTGRYDQLNPALLGTDATGLG
                     SYQTLVGLDFQYLLQVFKTHYLRTRFIASRLFSSNVHIDGLSSYGGSINTHGTVKPGI
                     ENNLDLAFEFTLTQHWVAVMEGYIATGKATRFNGILNFTSIGSPPVNVSDLDTAPVVI
                     GNGSFREEALAPAIEYNFNENIGVIGGVWFPVSGVNTSHYMTYVLAINAFW"
     gene            2269..>3844
                     /locus_tag="ACF2SP_002949"
     CDS             2269..>3844
                     /locus_tag="ACF2SP_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HFD2360709.1"
                     /translation="MKKNRFTYGLALGALGIVFGDIGTSPLYALKVTLSGIPINQFNI
                     LGVLSLIFWSLIIVVSFKYLMIIFRADNDGEGGILALLALMKHKSTKYQPVFYIVAIF
                     GAGLLLGDGMLTPAISVVSAVEGLGTLSDKLYPYVLPIASLILVLLFSLQAKGTARIG
                     YLFGPLILIWFITIAILGILQILEHPVVLKAINPYYAIAFLVDEGLRGYFLLGGIFLV
                     VTGGEALFADIGHFGKNPIRFSWFFIALPCLLLNYFGQGANLIVRPEEISNPFFMIAP
                     PWFYLPLIIIATVATVIASQAVISATFSLTKQAVLLGLCPKIPIVQTSMLHSGQIYVP
                     QINFILFIGTMAFCLAFKTSDNLAHAYGIAVNLEMLLVDAMVAYAAVSIWRWSTFNVI
                     FLFGLFLLIDLAFLGANTHKFITGGWVPIVLAFFIAFIMYSWRYGLEYLRDNFYMNKE
                     DISKILKQLQYKSLNQLPGVSAIFITDVYDKSGGSFLHFLKLNRSVPENVLIVNYIVD
                     NIPYVHYSQRYEIVCLD"
BASE COUNT         1075 a          800 c          716 g         1253 t
ORIGIN      
        1 gttcctttta taccaatgct aataaaacag ctgttaaata atttccctct ggaaaggctg
       61 ccaaagtagg atggcagctt gctggtccgt atacgcctaa aatacgcgcc tgctttccca
      121 tcgcaacagc ctgagcgctt accagggaac aaaaatcctg ggatgaaagt gctgaagaac
      181 agttgcaagt catcaataaa gatccaggtt tcatatattt aaatgcttct ctatgtaaaa
      241 atcggtaata atttttagct tgctgtaaat gtttttgtga aggaaccagt tttggcgggt
      301 caagaatgac cacatcatat tcgccagcca ttttcagata ttctctggca tctgccttga
      361 taaattcaat ggtatttaag tgattcaaaa cagcattatt tttagcctgc tctattgcct
      421 gcgccgagct atctacagcc gtaacttgaa gagcgcccgc tttcgctgcg tgcaaagcaa
      481 accctcccgt aaaagtataa agatccagga ctctttttcc cctggatagt tttgctatcc
      541 ttttgtgatt ttctctttgg tcaagaaaca atccagtttt ttgggtttgc gcaaattcaa
      601 catgatacag aacaccttct tccaaaacct gagtgctacc ctgacttttt tctgtatgaa
      661 tttctttcca gccatcttga gataacggct tgttttgagg aagccaaatg acatgactat
      721 gcgggaataa ctcttgcaat acctgaacaa taatcgattt attcaattct acccaataag
      781 cagaacttgc tataacgcaa tagtcattaa agcaatcaat cgttaaacca gagagcccat
      841 ccgcttcgct gttgaacaat cgataagcat tagtctgttc atttggtaaa tcaagacatc
      901 gtctcaccag actggcttgc ttaattctat gagtaattaa agacttgtaa tcgaaactat
      961 ttatctgttc attggagaga gcaagaaccc ttactctata taaagagtgc tcattatagg
     1021 ccccgaatcc taaactctca ccttcagagc caattatctc aaccaattca cctgtaatta
     1081 gcttgccaga atattgagct atggctttgg ggtaaatcca gggatgacct cttaatacag
     1141 tgttttgctt tccattcact aaaataactt tcgctttcat tctctatttc ccaaaaccaa
     1201 aaagtcagtt aatctacact atcaagaatt atacttgcaa tggaaagaag gacaaatatg
     1261 cggcaattta caaaatacct agccattttt ctattttcat cttcagtgag cgttgcttat
     1321 gccggtccct ggttcaccgg acctttattg gctcccgcag gccataccgt ccccaaaggc
     1381 cataccaacc tcgaagttta tggcattgat gtctttactg atgggcaata caatgcatct
     1441 ggacaaagaa tatccacacc caagtttaaa acctttgccg ccaacccaat actcactcat
     1501 ggctttactg actggctgga tgtgcagttg accgtgcctt atttattcaa ctcgacacgc
     1561 ggagttcatg agaaccattt ggctgatgtt gcaaccgcat tgggaataca aattctggaa
     1621 caaaaaagtt cccctaaaag aatcgatttt cgtgtccttt tacaggaaac aatacccact
     1681 ggacgttatg accaattgaa tccggcttta cttggtactg atgccactgg ccttggaagc
     1741 taccaaacct tagtgggctt ggattttcaa tatttattac aagttttcaa aacccattat
     1801 ctaagaacac gatttattgc atcacgtctt ttttcgagta atgtccatat tgatggacta
     1861 agcagttatg gtggctcgat caatactcat gggacggtta aacccggcat tgaaaataac
     1921 ctagacctgg cctttgaatt tactcttact caacactggg ttgccgtaat ggaaggttat
     1981 attgcaactg gtaaagccac tcggtttaac ggtattttaa actttaccag cattggaagc
     2041 ccgccagtca atgtcagtga tttggatact gcccccgtag ttattggcaa cggctcattt
     2101 agagaagaag cactggctcc ggcaatagaa tacaacttca atgaaaacat tggagtaatt
     2161 ggtggagttt ggtttcccgt gagtggagta aatacttccc attatatgac ctatgtactg
     2221 gcaattaatg ccttttggtg atagcacatg cgttattcct cttatgaaat gaaaaaaaac
     2281 cgattcactt atggccttgc ccttggagct cttgggatag tatttggtga tataggtact
     2341 agtccactgt atgctttaaa agttacttta tcaggcattc ctatcaatca attcaatata
     2401 ttaggcgtat tatcgctcat tttttggtca ttaatcatcg ttgtttcctt taagtacttg
     2461 atgattattt ttagggctga caatgatggg gaaggaggaa ttttggcact tcttgcttta
     2521 atgaagcata agagcaccaa atatcaacct gtattttata tagtcgccat atttggagcc
     2581 ggtttattat taggtgatgg gatgcttacc ccggccattt ccgttgtcag tgctgtagaa
     2641 ggtcttggaa ctctttctga taagctatac ccttacgttc tccccatagc ttcattgatc
     2701 ctcgttttgc ttttctcttt gcaagccaaa ggcacagcaa ggataggcta cctttttggg
     2761 ccacttatac ttatttggtt tataaccata gccatattag gaatattgca aattttagag
     2821 catccagtag ttttgaaagc aatcaacccc tactacgcca ttgcgttttt ggttgacgaa
     2881 ggcctgcgag gatacttttt gttggggggt attttccttg tagtaacagg aggtgaagcc
     2941 ttatttgcag acatagggca ttttggtaaa aatccaatcc gattcagctg gttttttata
     3001 gcgcttcctt gcctgctatt aaattatttt ggccaaggcg caaacctgat agtacgcccc
     3061 gaagaaataa gcaacccatt tttcatgatt gcaccaccat ggttttatct gcctttaatt
     3121 attattgcaa ctgttgctac agtgatcgca tctcaagcag taatttcagc aactttttca
     3181 ctaaccaaac aagccgtgtt gttgggttta tgccccaaaa ttccaattgt tcaaacctcc
     3241 atgctgcact caggacaaat ctatgttcca caaatcaatt ttattctatt tattggtacc
     3301 atggcatttt gcctggcttt caaaacctcg gataacctgg ctcatgctta tggaattgct
     3361 gtaaaccttg aaatgctttt agttgatgcc atggttgctt atgctgctgt ttcaatatgg
     3421 agatggtcaa cattcaatgt gatatttctc tttggtctat tccttttgat tgatttagcc
     3481 tttttaggag ctaatactca taaattcatt acaggtggct gggttccaat tgttttagct
     3541 ttctttatag ccttcattat gtacagttgg cgctatgggt tagagtattt aagagataat
     3601 ttttatatga acaaagaaga catctctaaa attttaaaac aattacaata taaaagttta
     3661 aatcaattgc ccggtgtttc agcgatattt attactgatg tttatgacaa aagtggtggt
     3721 agctttctcc attttcttaa actaaaccgc tcagtaccgg aaaatgtttt aattgtaaat
     3781 tatattgttg ataacattcc ttatgttcat tacagccagc gctatgaaat tgtttgcctt
     3841 gatg
//
LOCUS       DAXLDT010000235         3807 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXLDT010000235 DAXLDT010000000
VERSION     DAXLDT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.236"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..254
                     /locus_tag="ACF2SP_002950"
     CDS             <1..254
                     /locus_tag="ACF2SP_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360710.1"
                     /translation="TIKWRYGLIALFALGVSGCYFDGTSPQERGYSYQENSMPSSGGQ
                     TTASNQSQPQQKSYASKDTSQKSTPGPKRTAAPQLPVIQ"
     gene            552..1637
                     /locus_tag="ACF2SP_002951"
     CDS             552..1637
                     /locus_tag="ACF2SP_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD2360711.1"
                     /translation="MTLAYLSNYYPNLQVLFRIRKRVYIFQRDISKKIILLLLSWDFN
                     RFWDIIRLIPKGVILAEKLKPNTDYVLLKTDYGGFKNSWYIDDLKKKKINLIALVHDL
                     IPITNPEYCSIKNSLKFSKCLNSILDNAKGIVTVSEYTRSVLNDYVSQNKKSSPPTMT
                     ALLASGLSTCFAKEERIVKEKYFLTVSTIGDRKNHLLLLHVWRKLVEKMGGKAPKLII
                     VGKRSTSCNYTHALLERCSLLQGFVFETKCTDQELANYLYYARALLFPTFTEGFGLPL
                     IEALSLNVPVIASDIPVFHEIAGDIPDYLDPIDGKGWMEYIENYMLENSSLRQRQLDR
                     ISNFKIPLWDEHFAKVDAFIDLVMEAR"
     gene            1634..2554
                     /locus_tag="ACF2SP_002952"
     CDS             1634..2554
                     /locus_tag="ACF2SP_002952"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2360712.1"
                     /translation="MSEYPFSAKNTPPNCVDPDFYQAFQEHFRGSFDAIKERLTIYVS
                     YISELAKNYPEIPAVDLGSGRGEWLELLNEHGIGVYGIEKDNNLREVAERRGLTVVHG
                     DALEHIKQLPEQSRSVITAFHVVEHMTFEEMYCLVKEAHRVLVPGGMLLMETPNPENI
                     VVGSSKFYLDPTHTKPIPPEMLAFIPKYLGFFRVKIMRLNETVSLNKTKHLSILDVLG
                     GVSPDYCVLAQKEADTDLLQKTSFLFQETYGVRLEEIAEFYHRQFSKKLNLRNTRLLL
                     YKFINKLCRLFKNLLSLSKKVIFQCKDICR"
     gene            2673..>3807
                     /locus_tag="ACF2SP_002953"
     CDS             2673..>3807
                     /locus_tag="ACF2SP_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD2360713.1"
                     /translation="METRILIDASSLMNELKRKHPPHGIPRVTLAYLRCYYENMQLLF
                     RTSKRIFIFPQGISKKIADILLAWDCNRLWDIVRLILKGIIYSKKSKYESNYILIKTD
                     YGGLNNPGLLSVLQQNRLKMVALIHDLIPITNPEYFPLQHFEKFTQGITTILHNAKGV
                     VTVSEATRYILSDYISRAELPCPPIISANLAPGLAFSLSAQERPIAQPYFVIISSINE
                     RKNHLLLLHIWKRLIQKYGENSPRLVVIGYRHGECNYTYALMDRCPMLQEFVIERQCT
                     DEELSNYLHHALALLFPSFSEGYGLPLVEALSFKVPVIASDLAVFQEVAGDIPDYLDP
                     LDAISWMSCIENYILPDSPKRNAQLDRIKNFRVPSWMEHFDKVN"
BASE COUNT         1208 a          640 c          757 g         1202 t
ORIGIN      
        1 aaacaataaa atggagatat ggcctcattg cgctctttgc tttaggggta tcaggatgtt
       61 attttgacgg gacaagccct caagaaagag ggtattctta ccaggaaaat agcatgccat
      121 ccagtggagg acaaactacc gccagcaatc aatcacagcc gcaacaaaaa agctatgcgt
      181 caaaggatac ctctcaaaaa tctactcctg ggccaaagcg aaccgctgca cctcaactcc
      241 cggtaattca ataaaaaatg tcctattgat tatccctcat actcaagaag attatgaggg
      301 ataactgaaa cttgtttaaa agattttctt ttaaacgcaa agtgtcatta tgattgctga
      361 tacattgaag gtggcaggat agaatggctc atgtgattca tgataaaacg cattgcttct
      421 tgctgcgatg ggaactaaaa tccagactca gatttaattt gtacataatt ttaatgaaac
      481 aattattaat tgatattact agcttagcaa atgagcttag acgtaaacgt ccgccccatg
      541 gaattccgcg cgtgacacta gcttacttaa gtaattatta tccaaatctg caagtgttgt
      601 ttcgtatacg aaaacgcgtt tatatttttc aacgagacat ttcaaaaaaa attattcttt
      661 tgttgctttc atgggatttt aatcgttttt gggatattat ccgcttgatc cctaaaggag
      721 tgatattggc tgaaaagctc aaaccgaaca ctgattatgt gttattgaaa acagattatg
      781 gtggttttaa gaattcatgg tatatagacg atttaaaaaa aaagaaaatc aatttaattg
      841 ctctagttca tgatttaatt ccaattacta atcctgagta ttgttcgata aaaaatagct
      901 tgaaattttc taaatgcctt aactctatct tggacaatgc aaagggaatt gtaacagttt
      961 ccgaatatac tcgttctgta ctaaatgatt atgtttctca aaacaaaaaa tcaagcccgc
     1021 caacgatgac tgctttgttg gcttcaggat tatctacttg ttttgccaaa gaagaaagaa
     1081 tagttaaaga gaaatatttt ttaacagtca gtactattgg cgatagaaag aatcatttgc
     1141 tgttgcttca tgtttggaga aaattagtag aaaaaatggg gggtaaagcc cccaaactga
     1201 ttattgttgg aaaacgaagt actagctgca attatactca tgccttatta gaacgatgta
     1261 gtttattgca gggatttgtt tttgaaacaa aatgcactga tcaagagttg gctaattatt
     1321 tgtactatgc gagagcccta cttttcccaa cttttactga gggttttggt ttacccttga
     1381 ttgaagcttt gagtcttaac gttcctgtca tagccagtga tattcccgta ttccatgaaa
     1441 ttgcgggtga catccctgat tacctcgacc caattgatgg caaaggatgg atggagtaca
     1501 ttgagaacta tatgcttgaa aatagctctt tgagacaaag acaattggac cgtatttcaa
     1561 atttcaaaat accactctgg gatgaacatt ttgcaaaagt agatgcattt attgatctag
     1621 taatggaagc tcgatgagcg aatatccatt ttcagcgaaa aatacgcctc caaactgtgt
     1681 ggatcctgat ttttatcagg cgtttcagga gcattttcgc ggctcttttg atgcaataaa
     1741 agagagattg acaatttatg tttcttatat ttccgaattg gctaagaact atccagaaat
     1801 cccggcggta gatttgggct ctggcagagg agaatggctt gaacttttaa atgagcatgg
     1861 gattggtgtt tatggcattg aaaaagataa caatctaagg gaagttgcag aaagaagagg
     1921 ccttacggtt gttcatgggg acgcattaga acatataaaa caattgcccg aacaaagccg
     1981 tagtgtcatt actgcttttc atgtagtaga gcatatgact tttgaagaaa tgtattgtct
     2041 tgtgaaagag gctcatagag tcttggttcc aggcggtatg ttactaatgg aaactcccaa
     2101 tcctgaaaat atagtggttg gcagctcaaa attctatttg gatcctacac atacaaaacc
     2161 aattccacca gagatgcttg catttattcc caagtatttg ggatttttta gagttaaaat
     2221 catgcggctt aatgagacag tatcattaaa taaaaccaag catctgagca tactggatgt
     2281 tttaggtgga gttagccctg attattgtgt attggctcag aaagaggccg atactgattt
     2341 attgcaaaag acttcatttt tatttcagga gacatacggt gtcagattag aagaaattgc
     2401 agaattttat catcgtcaat tttctaagaa actgaattta agaaatacta ggttactatt
     2461 gtataaattt attaataagt tgtgccgctt atttaaaaat cttttatccc tttcgaagaa
     2521 agtgattttc caatgcaaag acatttgcag gtgagtattg tttaccagtg tagtctttct
     2581 gaaaggaaaa tattttgaat agaaagtgat tgaataacct ttttagcgca gttaatattt
     2641 gctgtcatga atagctaaaa gtgatgtagg acgtggaaac tagaatatta atagatgcca
     2701 gcagcttgat gaatgaactt aagcgaaagc atcctcctca tggtatccca cgagttactt
     2761 tagcctatct tagatgttat tacgagaaca tgcagttgct gtttcgaaca agcaagcgta
     2821 tatttatatt tcctcaaggc atttcgaaaa aaattgctga tattctacta gcgtgggatt
     2881 gcaatcgatt gtgggatatt gttcgattga tcctcaaagg tataatctat tcaaaaaaat
     2941 caaaatatga atccaattat attttgataa aaaccgatta tgggggatta aataatccag
     3001 gtttgttaag cgtcttgcaa cagaatcgtt taaagatggt ggcactaatc catgatttaa
     3061 ttccaatcac aaatccggag tattttcctc ttcaacattt tgagaaattt acgcaaggga
     3121 ttacaacaat tttgcataac gccaagggtg ttgtaactgt ttctgaagct acgcgatata
     3181 ttttatccga ttatatatcc agagctgaat taccttgccc gccaatcata tcagcaaatt
     3241 tagcaccagg tttggcgttt tcactatctg ctcaggaaag accaatcgcc cagccctatt
     3301 ttgtcattat aagctcaatc aatgaaagaa agaatcattt attattgctc catatttgga
     3361 agcgcttgat acaaaaatat ggggaaaatt cacctaggct tgtagttatc ggataccgac
     3421 atggcgaatg taattatact tatgccctca tggatagatg tccaatgctg caagagtttg
     3481 ttatagagag gcaatgtaca gatgaagagt tatctaatta tttacaccat gcgctggcat
     3541 tgctttttcc ttctttttct gaaggatatg ggctgccttt ggttgaggca ttaagtttta
     3601 aagtgccagt cattgcaagc gatttggcag tctttcaaga ggtggctggg gatattccgg
     3661 attatcttga tccactggat gctataagct ggatgagttg tattgaaaac tatattttac
     3721 ctgacagccc taagcggaac gcccaattgg atcgtataaa gaattttcga gtgccatctt
     3781 ggatggagca ttttgataaa gttaatc
//
LOCUS       DAXLDT010000236         3776 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXLDT010000236 DAXLDT010000000
VERSION     DAXLDT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.237"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..530)
                     /locus_tag="ACF2SP_002954"
     CDS             complement(<1..530)
                     /locus_tag="ACF2SP_002954"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360714.1"
                     /translation="MKYLTTPRLLLRAWDEEDTIPFYRMSQDPRVMKYFPGLWSMDMV
                     NDFIIRMNEQLSQKNFTLWAAEVKDCKQFIGFIGLNAPTWNAHFTPCVEIGWRLATEF
                     WGQGYATEGAKAALEYAFLEMHIPEIVSFTVPDNSRSRGVMDRLGMIRDIAGDFLHPK
                     LASDHRLAKHVLYRIQ"
     gene            complement(527..1315)
                     /locus_tag="ACF2SP_002955"
     CDS             complement(527..1315)
                     /locus_tag="ACF2SP_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFD2360715.1"
                     /translation="MKSLPIYQVDAFASNLFKGNPAAVCPLDKWLSDETMQAIAAENN
                     LSETAFFAPETDGYRIRWFTPNEEVALCGHATLASSYVLFNQLGFSGEEIIFNSLSGK
                     LWVKNTDQGLVMDFPVLPYEEINFSAELQHLNLKRPFKALRSQFDLMFVYQNAEEVVE
                     AKPDLNAVAHLDYRGLILTAPGISTDVYSRCFYPGCNVPEDPVTGSAHCVIAPYWCEQ
                     LRKNHITALQGSLRQGDLYCEVKENRVLLYGNCHLYLEGRIYLP"
     gene            1393..1812
                     /locus_tag="ACF2SP_002956"
     CDS             1393..1812
                     /locus_tag="ACF2SP_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD2360716.1"
                     /translation="MYLSSLLKKTNRLLIKRANELIKPYAMSHAYTYILMELYIEDGL
                     TQSELVQLIEVEQPTLVRTLDRMERDGFITRKPSSMDRRVVHIFLTEKARLLQEKIDE
                     CSRILNDEILAGFSEDEKTKLQELTHRVLDNLRNPIE"
     gene            complement(1842..2297)
                     /locus_tag="ACF2SP_002957"
     CDS             complement(1842..2297)
                     /locus_tag="ACF2SP_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFD2360717.1"
                     /translation="MPVKKLKQFLDSHKIKYLSIAHSPAYTAQEIAASAHVSGKQLAK
                     TVIIKMDGRLAMVVLPASDHITFMKLKEAIDASDLELATESEFEGKFAECDVGAMPPF
                     GNLYGLPVLVSTKLSAQDNILFNAGSHSELMQLSFGDFEKLVKPTLVTL"
     gene            complement(2457..3029)
                     /locus_tag="ACF2SP_002958"
     CDS             complement(2457..3029)
                     /locus_tag="ACF2SP_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HFD2360718.1"
                     /translation="MLLPIQVTFRNMDPSKAVESRAIKLAKKLDCLYDKIMGCRVVIE
                     SPHRHHNKGKLYHVRIDLTVPDAELVVSRESSNKHAHEDVYVSLRDAFNAIEHQLKNY
                     ANQRRGHVKRHEDSIRGGRVLEVCPIADYGYIETIDGRLIRFTGQSVVDYDINKLEVG
                     DRVRFVETVKNTQEAAASTVYVEGKRHIMN"
     gene            complement(3540..3773)
                     /locus_tag="ACF2SP_002959"
     CDS             complement(3540..3773)
                     /locus_tag="ACF2SP_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD2360719.1"
                     /translation="MSQRETGHVKWFNEKKGFGFIVNQKGEDIFVHYKDIQGVGFKTL
                     HENDPVTFELDRGPKGLKAQNVVVASESVHTEE"
BASE COUNT         1204 a          804 c          613 g         1155 t
ORIGIN      
        1 ttttgtatac gatataaaac atgctttgct aacctgtgat cagaggctaa tttgggatgt
       61 aaaaagtctc ctgcaatatc tctgatcatc ccaagtctat ccataacacc tcttgagcgg
      121 gaattatcag gcacagtaaa agaaactatc tcgggaatat gcatctccag aaaagcatat
      181 tcaagagctg cttttgcccc ttctgtagca tagccttgcc cccaaaactc agttgccaaa
      241 cgccatccta tctctacgca aggagtgaag tgagcattcc aagttggagc atttaatcct
      301 ataaaaccaa taaactgctt acagtccttt acttcagccg cccacaaagt aaagtttttt
      361 tgagataatt gttcattcat tctgataatg aaatcattga ccatatccat agaccacaac
      421 ccggggaaat acttcatgac ccgaggatcc tgactcattc tatagaaggg gatagtatct
      481 tcttcatccc acgctcttag caaaagcctt ggggttgtta aatatttcat ggcaaatata
      541 ttcttccttc caaataaaga tggcaatttc catagagcaa tactctgttt tccttcactt
      601 cacaataaag atcaccttgt ctgaggcttc cttgtaaagc agttatatgg ttcttcctta
      661 actgttcaca ccaataagga gcaataacac aatgagcaga tcctgttacc gggtcttctg
      721 gcacattaca tccaggataa aaacaacgag aataaacatc agttgatatt cctggagcag
      781 ttaatattaa ccccctatag tcaagatgag ccacagcatt caaatcaggc tttgcctcaa
      841 ctacttcttc tgcattttga tacacaaaca tcaaatcaaa ttggcttcgc aatgctttaa
      901 atggcctttt taaattcaaa tgctgcagtt cggcactgaa attaatttct tcatagggca
      961 gaactgggaa atccataacc aagccttgat ctgtgttttt aacccatagc ttaccactca
     1021 aactattaaa tataatttct tcccctgaaa agcccaattg attaaaaagt acataagagc
     1081 tggcaagagt ggcatggccg cacaatgcca cttcttcatt gggggtaaac caccggatcc
     1141 tgtatccatc ggtttcggga gcaaaaaagg ctgtttcaga taaattgttt tcagctgcaa
     1201 tggcttgcat agtctcatca cttaaccatt tatccaaagg acaaacagcg gcaggattac
     1261 ctttgaataa atttgaggca aatgcatcca cttggtagat tggtaagctt ttcataaatg
     1321 tctcctaaaa caacttgcga ataataacat agcctgttat tatatagcaa gctatttaat
     1381 aatgagaaat tgatgtatct ttcctctctg ctaaaaaaaa caaatcgttt gttaatcaag
     1441 cgagccaatg aattaattaa accctatgct atgagtcatg cctacactta tatattaatg
     1501 gaactgtata ttgaagatgg tttgacacaa tctgaattag ttcaattgat tgaagtagag
     1561 cagcctaccc ttgtaagaac tttggatcga atggaaagag acgggtttat cactcgaaaa
     1621 ccaagctcaa tggatagaag agtcgtgcat atttttctta cagaaaaagc ccgacttttg
     1681 caagaaaaaa tagatgaatg ctccagaata cttaacgacg agattctggc tggtttttca
     1741 gaagatgaaa aaacaaaatt acaagagcta actcatcgcg ttcttgataa tttaaggaat
     1801 cccattgaat aatttataat gatatttctc atcgcttcat tctatagcgt caccaatgtt
     1861 ggctttacta acttttcaaa atcaccaaaa gagagctgca tcaattcaga gtgagaaccc
     1921 gcattgaata agatattatc ctgtgcactc agtttagtcg acaccaacac tggtaaacca
     1981 tacaaattac cgaatggggg catagctcca acatcacatt ccgcaaattt cccttcaaac
     2041 tctgattcag tagcaagttc aagatcagaa gcatcaatag cttctttaag tttcataaat
     2101 gtaatatggt cacttgcagg caatactacc atagctaaac gtccatccat ttttataata
     2161 actgtctttg ccagttgttt tcctgaaaca tgagcagaag cagcgatttc ttgtgcagtg
     2221 tacgccggtg aatgagctat agacaaatat tttatcttat ggctgtccaa aaattgttta
     2281 agctttttaa ctggcatgac aaactccttt tcgatactaa tacattcttt cagaaaatgc
     2341 tctctctcaa gtgcccttat ttaaaatttt gaatcatttg tttggaaaca gtcatttcgc
     2401 ctaaacaatt actcaacagc aaactgcaaa ttaccaatag gcctctgata cccctgttaa
     2461 ttcataatat ggcgtttacc ttcaacgtaa accgtacttg cagctgcctc ctgtgtgttt
     2521 ttaaccgttt caacaaatcg tactcggtca ccaacctcta atttattgat gtcataatca
     2581 acgacacttt ggcctgtaaa tcgtatcaga cgcccatcaa ttgtttctat gtagccataa
     2641 tcagcaatgg gacatacttc aagaactctc ccaccacgaa tagaatcctc atgacgcttg
     2701 acatggcctc gccgttgatt tgcataattt ttaagctggt gctctatcgc attaaaagcg
     2761 tctcttagag aaacatatac atcttcatgc gcatgcttgt ttgatgattc acggctcaca
     2821 accaactcag catcaggaac tgtcagatca atacgtacat gataaagctt tcctttgtta
     2881 tgatggcgat gaggagactc aatgactact cgacaaccca ttattttgtc ataaagacaa
     2941 tcaagctttt tagcaagttt tatagccctg ctttcaacag ctttagatgg atccatattt
     3001 cggaatgtca cctgaatagg aagtaacatt tttaacctcc cttatgtttt gattatgaat
     3061 aatttcagat cttatttact aatctataga caaaaaaaat gttgctttca atagaaaaat
     3121 gataagcact tgaattaaca ataaaaataa aattattaaa accagactaa aaaaatggta
     3181 taaaaaatga attgagttga catacaaata cttcatatct gaaacttaat gagcatataa
     3241 caacaggacg ttgatgcttt ttttgggggg attgcatacg tggataagcc taagagttcg
     3301 acgctgcact ccgaagcaag atcgcctcag accattctct ttaacttaca ctcgtcctca
     3361 agtcagccac cttctccatt cattttagat ttggaacagc tgtacttttt gactcggcag
     3421 tagactataa aattaccgga ttttatgtca aactctcagg tttctataaa catgctctta
     3481 agtactttaa cgaggcttta aaaaataaag cgtagcctca ccagataaaa tcatctggct
     3541 tattcttctg tgtgtactga ttcgctagcc actaccacat tttgtgcttt caaaccctta
     3601 ggtcctcgat caagttcaaa ggtaactgga tcattttcat gcagcgtttt aaaaccaact
     3661 ccctgaatgt ctttgtaatg cacgaaaata tcttcgcctt tttgattaac aataaaacca
     3721 aatccttttt tttcattaaa ccatttaaca tggcctgttt ctctttgcga cattcg
//
LOCUS       DAXLDT010000237         3753 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXLDT010000237 DAXLDT010000000
VERSION     DAXLDT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.238"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            181..600
                     /locus_tag="ACF2SP_002960"
     CDS             181..600
                     /locus_tag="ACF2SP_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFD2360720.1"
                     /translation="MKALYTTIAKTHGGRNGHVETTDGLLKLDLAMPRELGGEGGATN
                     PEQLFAAGYAACFESAIRHVANVQKISLEDVSMTSEVSLYATPEKGFKLRVALHAYLT
                     GLNQNEAEALVAKAHEVCPYSNAIRGNVEVKLSVSVK"
     gene            635..1258
                     /locus_tag="ACF2SP_002961"
     CDS             635..1258
                     /locus_tag="ACF2SP_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFD2360721.1"
                     /translation="MYTLYSFGTPNGFKPTIMLEELAQPYTIKLIDITQGEQFNPDFL
                     KISPNNKIPVLYDQTNDFYLMESVAILQYLAEKHRQFLPETTKEKYAIIQWCYFQAAH
                     IGPMLGQFGHFHRYAQEQVPYAMKRYADESMRLLGVMEKQLAKTSFIGGSNYTIADMA
                     IWPWIWCFQFIYEQTIDAKLFPSLMSWYQRVSERPALKATLAAYGRL"
     gene            1693..3486
                     /locus_tag="ACF2SP_002962"
     CDS             1693..3486
                     /locus_tag="ACF2SP_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2360722.1"
                     /translation="MALSEIIINDVVQYQANAVISESNYHILRQYLLERKPLSRVLNP
                     INSLLSEHCDKDKQAIINHLTQLACESQRNYDIQEAMSDEQERVNENSLMINYRSELL
                     TLENTLKQLDTKCFQQQRQYNQVSRQFNELKVNLTHINQSIDRIRYERQLLDQQLNHP
                     YSDGNVHTNPPTHTRIYPDLSLPQAPLTRDRLLQEENRLIEERQRLMHLVNSKEAEKE
                     KEEQNLNRLHKEKKETEGRYSEVKHQIDIALPNSEQQRQIRNQERLAREQARASYDPH
                     LLQLSHKNLEALKREIEIQTRELDERRNQLMSEAKEMSYKIYLTQLEQALQESENGPQ
                     ITFNENNALKMILAMMKNMEEMAEKEKDIASSIEKERHNLHSLQKSLVAYTRQLEQYL
                     TSDPHLVKQNKSLTEENVQLQQYSESAASWRTKAYYASLFSVGGSLISTGILNTFIIS
                     PVFFAIPGALVALGVVSFVIAVVYHCQKYFSDVQMEINDQKIEKNELVLMKQWKKANE
                     LSLTTIPALNAKIEKSEKELYALEQKLQDQQHTMDLLLNKAQNITSNYYGSSNFFGNT
                     TGNVFYLPTAPLHEDSPLYPEIEGERVNYGY"
BASE COUNT         1259 a          670 c          711 g         1113 t
ORIGIN      
        1 gagactgatt ttctcaactt gtaactaatt taagtttgta tcgtcttttc tttgcttatc
       61 aaggctgttt tctttccagg cacgaattaa agtgcggcat gaaatttctt cctttctgtt
      121 tgacaaacag caaaatacta aataatttta atttcttacc attttaagga atataaaatt
      181 atgaaagcat tgtatacaac aattgccaag acccatggtg gacgaaacgg ccatgtagaa
      241 actacagatg ggctattaaa acttgatttg gcaatgccga gagagctggg gggggagggt
      301 ggtgccacta atccagagca attatttgcc gcgggatatg ctgcttgttt tgagagtgcc
      361 attcgtcatg ttgccaatgt gcaaaaaatt tctttagagg atgtttccat gacttccgag
      421 gtctctttat atgctactcc agagaagggt tttaaactta gagttgcatt acacgcctat
      481 ctaacaggcc taaatcagaa tgaggccgaa gcattagttg ccaaagcgca tgaggtttgt
      541 ccttattcca atgcaatacg tggtaatgtt gaagtgaaat tgagtgttag tgtcaagtga
      601 tagataactt ccatcaatta ataaggatat cttaatgtat acactttatt cattcgggac
      661 gcccaacgga tttaaaccca ctattatgtt ggaagagttg gcacagcctt acaccataaa
      721 actgattgat attactcagg gtgaacaatt taatcctgat tttttaaaaa tttcaccaaa
      781 caataagata ccggttttgt atgatcaaac caatgatttt tacctaatgg aaagtgttgc
      841 tattttgcaa tatttggctg aaaaacatag acagtttttg cctgaaacga caaaggagaa
      901 atatgccatt atccaatggt gttatttcca ggcagcacat ataggcccta tgttaggaca
      961 atttggtcat tttcatcgtt atgctcaaga gcaagtaccc tatgccatga agcgatatgc
     1021 tgatgaatcc atgagacttc taggggtcat ggaaaagcaa ctggccaaaa cgtcattcat
     1081 tggcggatca aattatacta ttgccgatat ggctatctgg ccttggattt ggtgttttca
     1141 attcatatat gaacagacta ttgatgcgaa actgtttccc agtttaatgt cctggtatca
     1201 gcgagttagt gaaaggcctg ctcttaaagc gactctagcc gcctatgggc gactatgatt
     1261 attggaacct atcgataatt aattgctccc aattgccata atgctgccaa ttgggatgtt
     1321 ttacctggtt attgcctcaa taaatagtga cattagaaaa attgagatta tgcaactgtc
     1381 gatcaaataa tctctattgt ctttcatatc tatttaaagc taagtttatt ctctaattct
     1441 gttattttga taattcttaa caattcctta acaatctctt aacaattcct taacatttgt
     1501 ctgccataat gccctcagtt taaacaaaag aataaaatac aggaggattt atcacactgc
     1561 tttgaataga actaggaaat gtagtctgta ttttcagggg aagcaaattt cttctgcatc
     1621 atgcagcgat tcacattaat agtttcaata aacgagttcg ttaataatat aattactgag
     1681 gagtcacgag tcatggcgtt atcagaaatc attatcaatg atgtagttca atatcaagcc
     1741 aacgcagtca ttagcgaatc aaattaccat attcttcgcc aatatctcct ggagagaaag
     1801 cctttatcaa gagtactcaa tccaatcaat tctctgcttt ctgagcattg tgataaggat
     1861 aaacaagcaa taataaacca cttaactcaa ctggcctgtg aatctcaaag aaattatgat
     1921 attcaagagg caatgagtga tgaacaagag agagtcaatg aaaactcttt aatgattaat
     1981 tacaggagcg agttgcttac cctggaaaat acattgaagc aactggatac aaaatgcttt
     2041 caacaacaac gccaatacaa tcaagtgagc aggcaattta atgaacttaa agtcaatctg
     2101 acccacatta atcaaagtat tgatagaatc cgatatgaac gtcaattgct tgaccaacag
     2161 ctaaatcacc cttactcgga tggtaatgtg catactaatc ctcctactca cacacgtatt
     2221 tatcccgatc tttcactacc ccaggctcct cttacgcgag atcgactgct gcaagaagaa
     2281 aatagattga ttgaggagcg ccagcggctg atgcatcttg tcaattcaaa agaagctgaa
     2341 aaagaaaaag aagaacaaaa tttaaataga cttcataagg agaaaaaaga gactgaaggg
     2401 cgttatagtg aagtaaaaca tcaaatcgat attgctttgc caaatagtga acaacaacga
     2461 caaatcagaa atcaagagcg tcttgctcga gaacaggcca gagcaagtta tgatcctcat
     2521 ttattgcaac tgtctcataa aaatcttgaa gcattgaagc gtgaaattga aatacagacc
     2581 cgggaactgg atgagcgacg aaatcagtta atgagcgaag cgaaagaaat gagttataaa
     2641 atctatctaa ctcaattgga acaggctctt caggaatcag aaaatgggcc tcaaatcacg
     2701 ttcaatgaaa ataatgcctt aaagatgatt ttagccatga tgaaaaatat ggaggaaatg
     2761 gcggaaaaag agaaagatat agctagctct attgaaaaag agcgacataa tcttcattca
     2821 ctgcaaaaaa gccttgtagc atacactagg caactagaac agtatctgac atcagatccg
     2881 catttggtga aacaaaacaa gtctcttact gaagaaaatg tacagttaca gcagtatagt
     2941 gaatcagcag ccagctggag aacgaaagca tactatgcgt ctcttttcag tgttgggggt
     3001 agtttaataa gtaccggaat attgaataca ttcatcatta gccctgtatt ttttgcgatt
     3061 cccggtgctc ttgttgcgct tggtgtagtt tcctttgtta tcgctgtagt ctatcattgc
     3121 cagaagtact ttagtgatgt gcaaatggaa ataaatgacc aaaagattga gaaaaatgaa
     3181 ttagtactaa tgaagcaatg gaaaaaggct aatgaattaa gtttaactac gattccagcc
     3241 ctgaatgcta aaatagaaaa atcagagaaa gagctgtatg cgcttgagca aaaattacaa
     3301 gatcagcaac acacaatgga tctgctgttg aataaagcgc aaaatattac cagcaattat
     3361 tacggaagca gcaatttctt tggtaacact actggtaatg tgttctattt acctactgcc
     3421 cctctgcatg aggactctcc actgtatcca gagattgaag gtgaaagagt aaattacggc
     3481 tactaataag ctaaaatgac tggtctttaa tttggtttca atttgaagac cagtatctct
     3541 gattattcaa atcctgaatt gctctttaca atctgccttg taaaaatatt ttgatcatac
     3601 tggatttaaa aagaagtatt ttttagttaa tgctgtttgg tttatgaggg ctggggctga
     3661 ttttaggact tacgaaaaat cccaatctct ttgttaaaaa aagtttttaa ttcggttatt
     3721 tagtttatct aaactccctc attaaaaaca ttt
//
LOCUS       DAXLDT010000238         3524 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXLDT010000238 DAXLDT010000000
VERSION     DAXLDT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.239"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            318..1637
                     /locus_tag="ACF2SP_002963"
     CDS             318..1637
                     /locus_tag="ACF2SP_002963"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFD2360723.1"
                     /translation="MKHQSNLSGRNVYVVDGSRTPFLKAKGVGPFCGSDLAVAAGTTL
                     LNRQPFSPDELDEVIIGSAMPGPDEANIARVVALRLGCGDKVPAFTVMRNCASGMQAL
                     DNAAIQIATGRSNLVLAGGTDAMSHAPLLFNEKMASWLANWFAAKSMGQKLALITKFK
                     PSYLAPVIALLRGLTDPIVGLNMGQTAEKVAYRFNITREQMDEFAAQSHLRIAQAYAE
                     NRMSEVAPIIDYKGCIYPQDDGIRADSTVEKLSKLKPFFDKKYGMVTPGNSSQITDGA
                     CLLLLASAEAVKKYALPVLGRIVDSQWAALDPSQMGLGPVHAATPILERHNLKPQDLD
                     CWEINEAFAAQVLGCLAAWSDEEYCKTHLGLKEAIGSPSLERLNRDGGAIALGHPIGA
                     SGARIVLHVLKSLEQRNESRGMAAICIGGGQGGAMYLERVTEVKGHE"
     gene            1630..>3524
                     /locus_tag="ACF2SP_002964"
     CDS             1630..>3524
                     /locus_tag="ACF2SP_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HFD2360724.1"
                     /translation="MNNYKHWDLQRDKDAILWLGLNRRDAAVNSINEEVLDELNSLLH
                     EISHDKKAMGLIIYSLKEKGFIAGADVNAFSQFESSSQAVDFLQKGQTVFARLQTLSI
                     PSVAMIDGFCMGGGYELALACTYRIATDDKDTRIGLPEVMLGIHPGWGGTVRLPQLIG
                     GFKALSQVILTGSAIPAVKAKSLGMIDEVVPVRQLRRAAVYFIKNKPPRHRASFIESL
                     TNSSLLRKALAYLLRYNVAKRVRKEHYPAPYAVIDLWEKEGGVGERAYLKEVDSIEKL
                     VSQNDTAKNLIRAFLLRERLKGFAKDSNFKATHVHVIGAGVMGGDIAAWCALRGLQVT
                     LQDKSYEQIAPAIGRAHKLYSKKLRKPRLIQAAMDRLIADPEGHGVKRADVIIEAVFE
                     NLAVKQEIMKIVEKNAKKDAIIATNTSSIPLDEISSVMSKPERLVGIHFFNPVAKMDL
                     VEIVSSSSTAKKIQQSSCAFVNQIGKLPLPVKSSPGFLINRVLMPYLMECVQLLDEGY
                     SAETIDEAALQFGMFMGPVELADTVGMDVCLAVAENLTSHFGGTIPQRLRDLVKEGKL
                     GRKTGEGFYKYKNGKVIKKKAPSHVDKTIADRLILRMVNESAACIREKVVEDSDLLDA
                     GMIFATG"
BASE COUNT         1078 a          595 c          861 g          990 t
ORIGIN      
        1 gaaatagtgc aaagaatatt gaggggagat cttttaacag cagaagcaat ttttcatgaa
       61 ttagctaaaa ttaatataga gagtcgtaaa gatgaattga ttttgcttag aaatgatttg
      121 caaagtgaaa aggtatgcgt aagaaagctt taacagaata aggcttgatg aataaacatc
      181 tccaggaatg atttacgtaa tttaatgtca tcaaaaaaat catcttaaga tttccaaaat
      241 atcaagaata ctgctaatct ttgaatctgt tgattttagt atagattctt atgcagaggc
      301 cctaacaagg aggactaatg aaacaccagt cgaatttaag tggtcgtaac gtttatgttg
      361 tcgatggaag tcgaacgcct tttcttaaag caaaaggagt aggtccattc tgtggttcag
      421 atttggcagt tgctgcagga acaactttgc taaatcgtca accattttct ccggacgagc
      481 ttgatgaggt cattattggt agcgctatgc ctggaccaga cgaggccaat attgctcgcg
      541 tggttgctct gcgtttggga tgtggtgata aggtgcctgc atttactgta atgagaaatt
      601 gtgcgtcagg catgcaggca ttggataatg ccgcaatcca aattgctaca gggcgcagta
      661 atttggtcct cgctggtgga acagatgcca tgagtcatgc accattgtta tttaatgaaa
      721 aaatggcttc ctggctggca aactggtttg cagctaaaag tatggggcaa aagctggctt
      781 taattacaaa gtttaaacct tcttatctgg caccggtaat cgctttgctc agaggcttga
      841 ctgatcctat agtaggattg aatatggggc aaacagctga gaaagtagct tatcgattta
      901 acatcacaag agagcaaatg gatgaattcg cagctcaaag ccatttaaga atagctcaag
      961 cttatgctga aaatagaatg tctgaagttg ccccaattat agattacaaa ggttgtatat
     1021 atcctcaaga tgatgggatt agggcagatt ctacggtgga aaaattgtca aaattaaaac
     1081 ctttttttga caaaaaatat ggcatggtca ccccgggtaa cagctcacag ataactgatg
     1141 gtgcctgttt gttgctattg gccagcgctg aggctgtaaa aaaatatgct ttaccggtat
     1201 taggtcgaat agttgattca caatgggctg ctctagatcc ctctcaaatg ggtttgggtc
     1261 ctgttcacgc ggcaacccct atattagaaa gacataattt aaagccacaa gatttggatt
     1321 gttgggaaat caatgaggcc tttgccgctc aagtcttagg gtgtttggct gcatggagcg
     1381 atgaagaata ctgcaaaaca catcttggcc ttaaggaggc aattggatcg ccatcgctgg
     1441 agcggctcaa tcgggatgga ggagcaattg cgttaggtca tcctattggt gccagtgggg
     1501 ctcgtattgt gttgcacgtt cttaaatctt tagagcaaag aaatgaatcg cgaggtatgg
     1561 cagcaatttg tatcggaggt ggtcagggag gagctatgta tcttgaacga gttactgagg
     1621 tgaaaggaca tgaataatta taaacattgg gatttacaac gagataaaga cgccattctg
     1681 tggcttggat taaatagaag ggatgctgct gttaatagca ttaatgaaga agtcctggat
     1741 gagttaaaca gcttgctgca tgaaatatcg catgataaaa aagcaatggg attaattatt
     1801 tattcgctca aggaaaaagg gtttattgcg ggtgctgatg ttaacgcttt ttcacaattt
     1861 gagagctctt cacaagcggt cgattttttg cagaaaggac aaactgtatt tgcccggttg
     1921 caaactttat caattccaag tgtcgcaatg atcgatggtt tttgcatggg cggtggttat
     1981 gagttagctt tagcctgcac ctatcgaatt gcaacggatg acaaagacac acgtattggt
     2041 ttgcctgaag tgatgctggg tattcatcca gggtggggcg gtactgtgcg tttgcctcag
     2101 ttaataggcg gttttaaagc tttatcacaa gtcatattaa ctggtagtgc aatacctgct
     2161 gtaaaagcta aaagccttgg catgattgat gaggtagtac ctgttcgtca gctaagaagg
     2221 gctgctgttt atttcataaa gaataaacca ccacgacaca gggcatcatt tattgaaagt
     2281 ttaacgaata gctctttgct tagaaaagct ctcgcttatt tgcttcgtta taacgtagcc
     2341 aagcgagttc gaaaagaaca ttatcctgca ccatatgccg tgattgacct gtgggaaaaa
     2401 gaaggaggag tgggagagag ggcttatctt aaagaagtgg actctataga aaaacttgta
     2461 tcccaaaatg ataccgccaa gaatcttatc agagcatttt tattgcgtga gcgattaaaa
     2521 ggatttgcca aagacagcaa cttcaaagcg acacatgttc atgtgattgg agctggagtt
     2581 atgggagggg atatcgcggc ctggtgtgct ttgcgaggtc ttcaggtcac attacaggat
     2641 aaatcttatg aacaaattgc ccctgcaata gggagagcgc ataaacttta tagtaaaaaa
     2701 ttgcgtaagc cgcgattgat ccaggcagca atggatagat taatcgcaga tcccgaaggg
     2761 catggggtaa aacgagcaga tgtcattatt gaggctgttt tcgagaattt agccgttaaa
     2821 caggaaataa tgaaaattgt ggaaaaaaat gccaaaaagg atgcgatcat agcgacaaac
     2881 acctctagta tcccacttga tgaaatcagc agtgtaatga gtaaaccaga gcgcttggta
     2941 ggcattcatt tctttaatcc tgtagctaaa atggatttgg tagaaatagt cagcagctca
     3001 tctactgcaa aaaaaattca gcagagctct tgcgcgttcg tcaatcaaat tggcaaattg
     3061 cccttgcctg tcaaatcaag ccctgggttt ttaataaaca gagtactcat gccttacctg
     3121 atggagtgcg tacagttatt ggatgaagga tatagtgcgg aaacaataga tgaggccgca
     3181 ttgcagtttg gtatgtttat gggccctgtt gaactggctg atacagtagg aatggatgtt
     3241 tgtcttgctg tggccgaaaa tctgaccagt cactttggtg gcactattcc acaaagatta
     3301 cgtgatttag taaaagaagg taagctaggc agaaaaactg gagaaggttt ttataaatat
     3361 aaaaatggaa aagtaattaa gaaaaaagcc ccgtcacatg tcgataaaac tattgcagac
     3421 aggctaattt tacgcatggt gaatgaatct gcggcttgta tacgtgagaa agtggttgaa
     3481 gattctgact tgttggatgc tgggatgatc tttgccacag gttt
//
LOCUS       DAXLDT010000239         3406 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXLDT010000239 DAXLDT010000000
VERSION     DAXLDT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.240"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            172..1197
                     /locus_tag="ACF2SP_002965"
     CDS             172..1197
                     /locus_tag="ACF2SP_002965"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFD2360725.1"
                     /translation="MGKAIKVIITQEQKQTLLKLRGSRTGKNIGERAYYVLLSSSGKS
                     IGEISAQLSRNPHTVRCWIKRYLHFGISGLIDKNSSGRPNQLREKVKEELNQLLSVSP
                     KEYGYQQSGWQINLQLDQLTKTVGSVSATTVKRALHEHGWVYKRYSKKPPLNAPSKED
                     KAAYIEKMVASLSKQKLEHEIEILFADESHFSNEPYVERGWFKRGEKKPVHTNKMRES
                     KTLFGALSLSSQAFYWKQAERGTLRYLFNFYINYIKAKPNKKIVMIIDNGSIHRSKKV
                     SKFVKKHEWVELFFLPPYSPEYNPIELFWKWLKRKIYGANGFTCIEQLIARIRKFIWH
                     YNENKFA"
     gene            2083..2997
                     /locus_tag="ACF2SP_002966"
     CDS             2083..2997
                     /locus_tag="ACF2SP_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFD2360726.1"
                     /translation="MKVITTAGNKGGIGKSTIALTLAQYLAKCKNMKGAFIDLDPQGN
                     SSSSLIPTTRDPAHPTGYMPKAHPEWDPNNLPEDDPHWDGVSSIADIFIGKSIYPYPT
                     WVDNLHCFPSFASLLEDAQRVLKVDVKEKVINRLKEFTEMLSTHSDYQFVIIDTNPQF
                     GPLTMAGLRAATHGLLPTELEQYGINGTIGMIQAISQEQLRRVKDDTIKIAGILPNQV
                     RKTAVHTKFLNDLRSIEKSEEWLLPPIAQRTIYSELVVEDAKPNCVFNLPQTHLARIE
                     SEKWCSYVYDRVFHNVYNKIEEKEASYG"
     gene            2990..>3406
                     /locus_tag="ACF2SP_002967"
     CDS             2990..>3406
                     /locus_tag="ACF2SP_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFD2360727.1"
                     /translation="MVSKFKLNPVKTGETKEAISRTLAIANDYAGELSIEVLSLDKIE
                     LDPENNRELALTLHDAMNGIDPSDPDYAKKKNDWKSLESLAKTILDDQLINPIFVYRF
                     GNKCRLIAGERRTLASAIAGKKEIIARIASQRPVGTK"
BASE COUNT         1272 a          588 c          604 g          942 t
ORIGIN      
        1 caagtagaga agctaatctc agctccaaca acaccagcga tcaatattcg ttaaaaaaat
       61 ataatcttcg ctatggacaa agacaaataa gcaaccatcc ataatctttt ataaaacata
      121 aagatagtgt aatgtccgca tcaactttta aggagtagct gagttttaag gatgggtaaa
      181 gcgataaaag tgattatcac gcaagagcaa aaacaaacac ttctaaaact aagaggctcg
      241 cgaacgggaa aaaatatagg ggagcgagcg tattatgttt tactgtcctc atcaggcaaa
      301 agtataggtg aaatctcagc gcaattgagt cgtaatccac acacggtgag atgttggata
      361 aaacgttatt tacattttgg catttcagga ttaatagata aaaattcaag cggtcgaccg
      421 aatcaattga gagaaaaagt caaggaagag ctcaaccaat tgctaagtgt atcgcccaag
      481 gagtacggtt accaacagtc tggatggcaa attaatcttc aattagatca attgactaaa
      541 acggttggct ctgtgagtgc gacaacagta aagcgagcac ttcatgagca tggatgggta
      601 tataaacgct attctaaaaa gcccccactg aatgcgccga gtaaagaaga taaagcagct
      661 tatattgaaa aaatggttgc atcgctaagt aaacaaaagt tagagcatga aatagaaata
      721 ttatttgcag atgagtctca cttctcaaac gaaccctatg tagagagagg ttggtttaaa
      781 cgtggcgaaa aaaaacccgt ccacaccaac aaaatgagag aaagtaaaac attatttggt
      841 gctctgagtt taagctcaca agctttttat tggaaacaag ccgaaagagg aactctaaga
      901 tatttattca atttctacat caattacata aaagctaaac ccaacaaaaa gatagtgatg
      961 attattgata atggctctat tcatcgcagc aaaaaggttt caaaatttgt gaaaaaacac
     1021 gaatgggtgg agttattttt tttaccacca tattcacctg aatataatcc aattgagcta
     1081 ttttggaagt ggttgaagag gaaaatatat ggtgcgaatg gatttacttg tattgagcaa
     1141 ttgattgctc gtattagaaa atttatctgg cattacaatg aaaataaatt tgcttgaccc
     1201 tattcaattt gagtttaacg catatcggga gttgttataa atgtttttgg atggttactt
     1261 ataatagatt taactcaata cgtggaccct tattacgggc atatatagct gaataaccaa
     1321 gatacttagc aagaattaat cacaaactaa caccgctaca aagtagcaat tggtatgatt
     1381 atttaatcgc aaatgaagtt attctgttac aactagaaat gaaatctaga atatggaaat
     1441 aacattcgtt tatatctatt cccgaattta ttctattatc aaagttaatc aatgtgcgac
     1501 ttattcgagc aaacgcaaaa agtatagtgt ggtactatcc agttaaacat atcttattgc
     1561 agggtagaga tttaatacaa aagataagta tacccaaaca aacatcagga taatctttta
     1621 ctgagattcg caataccact tgccactccg aattttcaaa gtttctagag caaaagatcc
     1681 agccgcctaa aatctagcat tcattgcatt aggtagatta tggataaaag ttaaaatctc
     1741 tcccataaat ctacaccatt atcatataac tacaccacaa taccccataa agctacacat
     1801 atgattaaaa aaccttttga accccatatt actacacaga aaaactcata aacctacact
     1861 atcactccca aatagataca caattatcct ttcataaatc atttaaaatc aattagttac
     1921 tgtgttttat ataaggaatc tatgaataga agaaaaaaga tatatatttt tcttttttat
     1981 aaatttaaaa gaatagtaca aattaccgct tgcaaattca tttcaaagaa gatagcctaa
     2041 taacaaatag tacaaaacac cttattgtaa gaagggggat ggatgaaagt aattacaaca
     2101 gccggaaaca aaggagggat tggcaaatca actattgcac tcactcttgc acaatatcta
     2161 gctaaatgta aaaacatgaa aggggcattt attgatttag acccacaagg taattcttca
     2221 tctagcctaa ttccaactac cagagatcct gctcacccaa cgggttatat gcctaaagcg
     2281 catccagagt gggatcctaa taacttacca gaagatgatc cacattggga tggtgttagt
     2341 agtattgcag atatattcat aggtaaatct atttatccat acccaacatg ggttgacaat
     2401 ttacattgtt tcccttcttt tgcttcatta ctggaagatg cacaacgtgt tctaaaagtt
     2461 gatgtaaaag aaaaagttat aaatcgtctt aaagaattta ccgaaatgct gtcaacccac
     2521 agtgattacc aatttgttat tattgatacg aatccacagt ttggcccact aacaatggca
     2581 gggttgcgag cagcaactca tggactattg ccaaccgaac tagaacaata tggtattaat
     2641 ggaaccatag gtatgatcca agcaatttca caagagcaac ttagaagagt aaaagatgac
     2701 acaatcaaaa tagcgggaat tttacctaac caagttcgta aaacagcagt acatactaag
     2761 ttccttaatg acttgcgttc gatagagaaa tcagaagaat ggttgttacc tccgatcgcc
     2821 caaagaacta tttattcaga attggtagta gaagatgcaa aacctaattg tgtatttaat
     2881 ctaccgcaaa cacatctagc tagaattgag agtgaaaaat ggtgttctta tgtatatgac
     2941 agggtatttc ataatgtata caacaaaata gaagaaaagg aggcttctta tggttagtaa
     3001 attcaaacta aatccagtca aaaccggaga gacaaaggaa gccatatcaa gaacactggc
     3061 gattgccaat gattatgctg gggaactgtc aatagaagta ttatccttag ataaaataga
     3121 acttgatcca gaaaacaatc gtgaactagc actaactctt catgatgcta tgaatggaat
     3181 agatccttct gatcctgact atgcaaaaaa gaaaaatgat tggaagtcac ttgagtcatt
     3241 agcaaaaact atactagatg atcagttaat taaccccata tttgtataca ggtttgggaa
     3301 caaatgtcgt ttaatcgctg gtgaacgtag aaccttggcc tcagcgattg ctggtaaaaa
     3361 ggaaattata gcaagaattg caagtcaacg acctgttggt acaaag
//
LOCUS       DAXLDT010000240         3399 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXLDT010000240 DAXLDT010000000
VERSION     DAXLDT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.241"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(72..1721)
                     /locus_tag="ACF2SP_002968"
     CDS             complement(72..1721)
                     /locus_tag="ACF2SP_002968"
                     /inference="COORDINATES: protein motif:HMM:NF036739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360728.1"
                     /translation="MKLPFKEPVAPRYIYINPQTNVVHLLMPVMSGTEIGLDNTCKSV
                     YSLLEFFCLLGANQQSAASMILKNYQEGLAFDIKYHPNSEQKALKEQRLVQINMYLRL
                     LQQVQQEEQITNPLKLIFPTYPAALESLMQASEANLYSVILRPEEQDVQLRTTAISPV
                     FSAHHDTMVNGQRIEKKSSLYEILSNSYEGLVFIPKSKEQLIERVLSKCADSPVDFEH
                     IRAMLTQETKAYLGIEESFNQTQGNRYAPSAPVNQAYIDGELMISAEYPATPQDYLNA
                     LLEYCTPNLFDNIEESPFYTINNKERLSILTQFFLAELNIICREEGIAETNFGQILEA
                     NPELINNLAKCVKQALTSYQSVEDALINYVNQHRDDFQFTSPIPQYSFPKLKERFNSH
                     YKQIKDSPHFDEFMLLSTKKGLFFAHQGCIAVHFACFMNALFFNDILDENTKTFLQSV
                     QQDFETIDKFENTIPHQNTHINAGMKEAVLDLSSMDNEALQDLYEDINSYQDSNLKEA
                     LLAQLKQERPDFKLQMNAKIFLQHVAYGEQDEAEELLNKDP"
     gene            complement(2021..3124)
                     /locus_tag="ACF2SP_002969"
     CDS             complement(2021..3124)
                     /locus_tag="ACF2SP_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFD2360729.1"
                     /translation="MKILKFLCSIVFSLILIATTLNAADIDYIIDTDIGGDIDDVLAL
                     FVALDNPVKPLAITTTHIEPREKAQIAKLVVTYAGFSEIPVYAGIGTTRNDPRELFLQ
                     QNPLWPPFFGYPDSLPGDSPWFPQQAVPYRQMNHSIFDEMNIEEESAPEYLARIAKNY
                     SPQHKLVIVSLGPLHNISAALKINPSISSNLKIYSMGGIYPKGYNWLVSPEVTAPVLG
                     TTEVISIVSQFVDAHDFYITPEEFLEIEKSAQSEFGLAVIQDWKNWYNIDKRNLNKTH
                     LSDPVTLYLALHPEEITHMSSKLLTFPCLDLNGVLAPEFAGSWYNMPGLENKLMKVED
                     SEESHIKFVSQVESSSAIRRKIINSIKQILGNS"
BASE COUNT         1032 a          616 c          662 g         1089 t
ORIGIN      
        1 gtgcaagtaa aggtgcggcc tgagtaatcg gtgaagaaaa tgttattggc cctcaagagc
       61 tcctgggtga gttacgggtc tttgtttagg agttcctctg cctcgtcttg ctcaccataa
      121 gcaacatgct gtaaaaatat tttggcgttc atttgcagtt taaaatcagg gcgttcttgt
      181 tttagttggg ctaaaagcgc ttctttgagg tttgaatcct ggtagctgtt aatatcttca
      241 tacaaatcct gcaaggcctc attatccatt gaggataaat caaggaccgc ttccttcatg
      301 cctgcattaa tatgcgtatt ttgatggggt attgtatttt caaatttatc aattgtctca
      361 aagtcttgtt gtacgctttg caagaaagtt tttgtgttct catctaatat atcattaaaa
      421 aacaaagcgt tcatgaagca ggcaaaatgg acggctatgc atccttgatg agcgaaaaat
      481 aatcctttct tcgtgcttaa taacataaat tcatcaaaat gtggtgagtc tttgatttgt
      541 ttgtaatgag aattgaagcg ttcttttagt tttggaaagc tatattgtgg gatagggcta
      601 gtaaattgaa aatcgtctct atgctgattg acataattaa taagtgcatc ttctacagat
      661 tgatagctag tcaacgcttg ttttacacat ttggccaagt tattaatgag ttcaggattt
      721 gcttctaaaa tctggccaaa atttgtctct gctatgccct cttcacgaca tatgatgtta
      781 agctctgcta aaaaaaactg agttagaata gatagtcttt ctttgttatt tatcgtgtaa
      841 aaaggggatt cttcgatatt atcaaacaga ttaggagtac aatattcaag taaagcattg
      901 agatagtctt ggggcgtggc aggatattct gcactaatca tcagttcccc atcaatgtag
      961 gcttgattta ctggagcaga aggagcataa cgattacctt gggtttgatt gaagctttcc
     1021 tcaatgccaa gataggcttt ggtttcttga gttaacattg cgcgaatatg ttcaaaatcg
     1081 acgggtgagt ccgcgcactt cgataaaacc cgttcaatca actgttcctt ggacttgggg
     1141 ataaaaacta acccttcata gctgtttgat aatatctcat aaagcgatga ctttttttca
     1201 atcctttgac cattgaccat agtatcatgg tgggcactga atactgggga tattgctgtc
     1261 gtacgtaact gaacatcctg ctcttcaggc cgcaatatca cactgtacaa gtttgcttcg
     1321 ctagcttgca ttaagctttc taatgcagca ggataggtgg ggaatataag ctttaagggg
     1381 tttgtaatct gttcttcttg ttgaacttgt tgcaacagtc ttaaatacat attgatttgc
     1441 acaagacgtt gctctttaag tgctttttgc tccgaattag ggtggtattt tatatcaaat
     1501 gccaatccct cttgataatt cttaagaatc atagatgctg cactttgttg attggcgcct
     1561 aaaagacaaa aaaactccag tagggaatag accgacttgc atgtgttatc aaggccaatt
     1621 tctgtaccac tcatgacagg cattaataag tgaaccacat tcgtttgagg attgatgtaa
     1681 atatatcttg gagctaccgg ttccttgaag ggtaatttca ttgtattaac ctgaatttgc
     1741 tatgtaaaat caatatatta tcatcactac actggagtag cataagttaa tacaacatct
     1801 taaggcaaaa ttggtattca acttttaaca aactgcgtat gtattaagga gggaataagg
     1861 gtcagatcct gaatgcttat atattaaaga agagagcact gggaaactca agcagccagc
     1921 tcagactctt cgcggacgct ctttgaatct gtttagaaaa gccaagctga gctgaaaagc
     1981 ctgagggatc ctcgggctta ccagctcagg ctacctttgc ttaagaattg cctaaaattt
     2041 gttttattga attaattatc tttcttctaa ttgctgatga ggattcgact tgagatacaa
     2101 atttaatatg gctttcttcg ctatcctcta ccttcatgag tttattttca agaccaggca
     2161 tgttatacca agaacctgca aattcaggag ctaaaactcc attaagatca aggcatggaa
     2221 aagtaagtaa tttagacgac atgtgggtaa tttcttctgg gtgcaaagcc aaatacaagg
     2281 taacaggatc tgataaatgc gttttgttaa gatttctctt gtcgatatta taccaattct
     2341 tccaatcctg aattactgcc aatccaaact cggactgtgc acttttttca atttccaaaa
     2401 attcttcagg ggtaatataa aaatcatggg catcgacaaa ttgactgact atagaaatca
     2461 cttcagtagt gccaagcact ggagctgtaa cctcagggga aaccaaccaa ttatatcctt
     2521 tgggataaat accgcccata gaatatattt tcagattaga actaattgaa ggattaatct
     2581 ttaacgccgc agagatgtta tgtaatgggc ctaaagagac aattactaat ttatgttggg
     2641 gtgaataatt tttagcaata cgagccaaat attctggggc tgactcttcc tctatattca
     2701 tttcgtcaaa aattgaatga ttcatctgcc tgtaaggcac tgcttgctga ggaaaccatg
     2761 ggctatctcc tggtaatgaa tcagggtaac caaaaaaagg aggccatagc gggttctgtt
     2821 gtaaaaatag ctctctggga tcattacgtg tagtgccaat tccagcatac acaggtattt
     2881 ctgaaaatcc ggcataagtc accacaagtt ttgcaatttg tgccttttct ctcggttcaa
     2941 tatgcgttgt ggttattgcc aagggcttaa ctggattatc aagtgctaca aacaatgcaa
     3001 gtacatcatc aatatcacca ccaatatctg tatcgataat ataatcaata tctgcagcat
     3061 ttagggttgt agcgatcaga attaaactga atacaatcga acataaaaac ttaagtatct
     3121 tcatgaaaat caaaatcctt aatgactctc aaaacgacaa aaccaaatat ttatccaagt
     3181 ccgacaaata tacgcttaaa gtctatagtt gactatttta ataagggaat ttaacaagta
     3241 agtcggggca ttttgtttgt aattttcata aggccaatta ctaataaaga tcagatttaa
     3301 ctgcttttta gctctacccc aacaaagccc ttttaaatca ttagttgcct tgggaaagac
     3361 ccgatttaac tcgttgatct aaaagggttt atgatggtc
//
LOCUS       DAXLDT010000241         3386 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXLDT010000241 DAXLDT010000000
VERSION     DAXLDT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.242"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            19..2037
                     /gene="legC6"
                     /locus_tag="ACF2SP_002970"
     CDS             19..2037
                     /gene="legC6"
                     /locus_tag="ACF2SP_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC6"
                     /protein_id="HFD2360730.1"
                     /translation="MSHSKREQNPIARNLSDFRQVATLLNNEGLVVTEHNYKKVQALL
                     NRYKELATFNDQNQSKLNGKQIGELNQIRLIISPEDRREIRKSVTRIENEIKNREKRI
                     KEEYTRIEKQQAKAREAVQVLWPQLQKAAKKFINSGIANMEELLKEYKELRNELYNHA
                     LNLPTQERKQKNQEMAELSRLVNIHESKLYEEINRARKVIEAAVTQAQKNVDSMSLLQ
                     MVRLMLPKLKDADILKIAQKAGVLSLQDTTTSGHGANLDKLVVREQPLEKQSALISIM
                     FKELALNGAQAGPLAKYLQAVQENAKDLISGKKTHFTLLYQTNEGEIGGFYFNIHELL
                     QNQHQAISKLVHEMYKRGVDQNSRVVMAFASPEQAASLMLANGENIKALTEAGNLQEL
                     TEQLLENAGDSKALARIVGHDVVIGQKTSEIIRDLNRSKEQNGIEPAEVMKLFQIIAV
                     IFETRNILINWITAPVHLKEQTEEALLGAGKTPAIVEGKGPQPKRVRFLPDLVEKPKA
                     TLTDTEQPVTKRKDDELLDKKIQVALEKVDQALEELKGKIGDINQHKYDKAYEAAKIL
                     LRQLQETRDQYAIDLANPQINFKEAGLKFKEASNLVIQQAKPILEKDLGWGEYLKNLF
                     KVIVNAIVSCVTFGASQGFFATTRAKSAEAVEKAESELELNQLGSLPK"
     gene            complement(2133..2690)
                     /locus_tag="ACF2SP_002971"
     CDS             complement(2133..2690)
                     /locus_tag="ACF2SP_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFD2360731.1"
                     /translation="MARTLSNMLPLGTVAPHFELLDVVSGSKVKLRETPRYVATVIMF
                     ICNHCPFVKHINNELTQLANDYIPQGIRFLAINSNDVESYPDDSPENMIQTAKVHQYP
                     FPYLFDETQEVAKAYNAACTPDFFVFDSDLSLVYRGQLDDSRPGNAIPVTGNSIRQAL
                     DCLLNNKPFDFEQKPSIGCNIKWKI"
     gene            complement(2715..3215)
                     /locus_tag="ACF2SP_002972"
     CDS             complement(2715..3215)
                     /locus_tag="ACF2SP_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360732.1"
                     /translation="MSKTKYDTIYLVLGIVGAFLLIYGLSQNNPQVYYITGSLALMIT
                     AIHYKLIYFIALELILGAGHTSLLLGMGRYIGLALPSLLCLQLLIFYLMFGKGNSIFL
                     LTGIIGIALLSMGLAYHNQIIFFSGSSGVAAYSYYSAYRGQYICYIWGILNTIFASYA
                     LYQLVF"
     gene            complement(3219..>3386)
                     /locus_tag="ACF2SP_002973"
     CDS             complement(3219..>3386)
                     /locus_tag="ACF2SP_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhB"
                     /protein_id="HFD2360733.1"
                     /translation="DENTALVDCRDVLEVCQYPRVKFALSNMGNYWISTNKSQNEVIQ
                     ESVAKGIYLKW"
BASE COUNT         1263 a          564 c          630 g          929 t
ORIGIN      
        1 ccatcaagga atcattctat gtctcattcc aaaagagaac aaaatcctat tgcccggaat
       61 ttaagtgact ttaggcaagt tgcaacacta ttaaataacg agggtcttgt tgttactgaa
      121 cataactata aaaaagtcca agccttatta aatagatata aagagctagc aaccttcaat
      181 gatcaaaacc aaagcaaatt aaatggaaaa caaataggtg aattaaatca aattagatta
      241 attatttctc cggaagatcg gcgagaaatc agaaaaagtg ttacacgtat cgaaaatgaa
      301 ataaaaaata gagaaaaaag aataaaagaa gaatatacga gaatagaaaa acaacaggct
      361 aaggcaagag aagcagttca agttttatgg ccacagttgc aaaaagcagc caaaaaattt
      421 ataaactctg gaatagcaaa catggaagag ctattgaaag aatataaaga attaagaaac
      481 gagttatata atcatgcgct taatcttcct actcaggaaa gaaagcaaaa aaatcaagag
      541 atggctgagt tatctcgtct tgttaatata catgaaagca aattatacga agaaattaat
      601 cgagccagga aggtaattga agctgcagtg actcaagcac aaaagaatgt agacagtatg
      661 tcattgctgc aaatggttcg actcatgctg cctaaattga aagacgcaga tatattaaaa
      721 attgctcaaa aagccggagt actttccttg caggatacaa caacctctgg acatggggca
      781 aatttagata aattggtcgt aagagaacag ccattagaaa aacagtctgc tctaatttct
      841 ataatgttca aggaattggc tctcaatggt gctcaggctg gtcctctggc aaaatacctc
      901 caggcagttc aagaaaatgc aaaagatcta ataagtggta aaaaaactca ctttactctg
      961 ctttatcaaa caaatgaggg tgaaattgga gggttttatt ttaacattca tgaattgttg
     1021 cagaatcagc atcaagcaat ttccaagctt gtacatgaaa tgtataagag aggagttgat
     1081 caaaatagtc gagttgtaat ggcttttgct agtccagaac aagctgcgtc tttgatgtta
     1141 gctaatggag aaaatattaa agcattaact gaagccggta atttacaaga gttaactgaa
     1201 caattattag aaaatgctgg tgattcaaaa gcattggctc gcatagtagg acatgatgtt
     1261 gtaattggcc agaaaacttc agaaataatt agagatctta atcgttcaaa ggagcaaaat
     1321 ggtatagagc cagctgaagt aatgaaatta tttcaaataa ttgcagtcat atttgaaact
     1381 cgaaatattt taatcaattg gataactgct cctgtgcatt tgaaggagca aacggaagaa
     1441 gctttattgg gcgcaggtaa aactcctgcc atagttgagg gcaaaggacc ccaacccaaa
     1501 cgcgtaagat tcctacctga tttagtggaa aagccaaaag ctacattgac agatactgaa
     1561 caacctgtta ccaaaagaaa agacgatgaa ctacttgata agaaaataca agtagcatta
     1621 gaaaaggtgg atcaagcatt agaagaactg aaaggtaaaa taggggatat caatcaacat
     1681 aaatatgaca aagcctatga agcagcaaaa attcttctaa gacaattgca agaaacacgt
     1741 gatcaatatg ctattgattt agctaatcct caaatcaatt tcaaagaggc agggcttaaa
     1801 tttaaagaag ctagtaacct ggtgattcaa caggccaaac ccatcctgga aaaggattta
     1861 ggttggggag aatacctcaa gaacttgttt aaagtgatag taaacgctat tgtatcttgt
     1921 gtgacttttg gagcaagtca aggcttcttt gctacgacac gtgcgaaatc tgctgaagca
     1981 gtggagaaag cggagagcga gctggaacta aatcaactcg gttcattacc aaaataatac
     2041 atatggttaa ctagtttttt atgagagcaa cattcgattt ttattataga tataaatcga
     2101 tgttgctttt ttgaattaaa gatagcttca gcctatattt tccacttgat gttgcatcca
     2161 atacttggtt tttgttcaaa atcaaaaggt ttattattta ataagcaatc caaagcctgg
     2221 cgtattgagt tgccagttac aggaatagca tttcctggtc ttgaatcatc taattgccct
     2281 cgataaacta aagacaaatc agagtcaaaa acaaagaaat ctggtgtgca agctgcatta
     2341 tatgcttttg ctacttcctg ggtttcatca aaaagatatg gaaatggata ctgatgaact
     2401 ttagcagttt gaatcatatt ttcaggtgag tcatcagggt aactctctac atcattggag
     2461 ttaatggcta aaaaacgaat cccttgggga atatagtcat tagcaagttg tgttaattca
     2521 ttattgatat gttttacaaa gggacaatgg ttgcatataa acataatcac agtagccaca
     2581 taacgtggag tctctcttaa tttaacttta cttccactaa ctacatccaa taattcaaaa
     2641 tgaggagcta ctgtacctaa tggaagcatg tttgatagag ttctagccat tttattaaat
     2701 ccctcagata atctttaaaa taccaattga taaagagcat atgacgcaaa tattgtatta
     2761 agaatgcccc atatgtaaca gatgtattga cctctatacg cactataata agaatatgca
     2821 gctacgcctg aacttccaga aaaaaatatg atttgattat ggtaggcaag ccccatcgaa
     2881 agcagtgcta ttcctataat gccggttaat aaaaaaatac tgtttccctt gccaaacatt
     2941 aaataaaaaa tcagtagctg taaacacagc aaagaaggta aagctaatcc aatatatctc
     3001 cccataccaa ggagtaggga ggtatggcct gcccccaaaa ttaattccag agcaataaaa
     3061 taaatcaatt tatagtgaat tgctgtaatc atgagagcaa gtgagccagt aatgtaatag
     3121 acttggggat tattctggct cagcccataa ataagtaaaa aagcaccaac aatacctagt
     3181 accagatata tcgtgtcata ctttgtttta ctcacggatc accattttag gtaaattcct
     3241 ttagcaaccg attcctgtat aacttcattt tgtgatttat tagtagaaat ccaatagttt
     3301 cccatattac taagtgcaaa tttaacacga ggatattggc agacttcgag aacatctcga
     3361 caatcaacta aagcagtatt ttcatc
//
LOCUS       DAXLDT010000242         3362 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXLDT010000242 DAXLDT010000000
VERSION     DAXLDT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.243"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            367..3243
                     /gene="rapA"
                     /locus_tag="ACF2SP_002974"
     CDS             367..3243
                     /gene="rapA"
                     /locus_tag="ACF2SP_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFD2360734.1"
                     /translation="MTFRIGQRWISNTESQLGLGIITEVDGRQVSISFPAAGEDRIYA
                     IDNAPLSRIVYKTGEEILTNDQQRIKVTSIQEQDGVLLYTGNDDLGNVAQVSELALNC
                     FIKLNSPQQRLFSGLLDKLNAFKLRIKTLEHLGRLQQSKARGLLGSRTNHLIHQIYIA
                     YEVAQRFAPRVMLADEVGLGKTIEAGMILHYQLHTGRASRVLIIVPNSLIHQWFVEML
                     RRFNLHFSIIGPNQYDAMPTTLDELEDNDNPIEPASEDGNLFEDEQLVLTSLDFLMEN
                     ETARQQALAAQWDLLVVDEAHHLYWSEDSQSPEYTFIEKLSARSTGLLLLTATPEQVG
                     MKSHFARLRLLDPARFYDFSAFVNEEKQYHEINKIVQDLMDYKEKNGVDELNPQLHSH
                     LKQLLGKEAPAGINATIKELLDRFGTGRVLFRNTRAAIKGFPERRIHPVALTCPALYS
                     EIIRESNLIHLYPETLLNNDSWIEHDPRVQWLVNKIDELRPEKILVICAKAKTAISLE
                     QYLKLKTGIRSTLFHEGLTIIERDRAAAYFAEQENGAQVLVCSEIGSEGRNFQFSHHL
                     VLFDLPLNPDLLEQRIGRLDRIGQNHPIEIHVPYLAGTAQEKLFRWYHEGINILQQSC
                     SVGFSIFEVFENRLTPVLSNPLQDTSEQSLEKLIADTQLHTQHIKEALNAGRDRLLEL
                     NSCNYTIGKELIEAIEAEENCLELENYMSQVYQEYGIDQEYHSENAEILRPGNHMKTA
                     HFPGLKEDGMTVTYSRSKALVREDMEFLSWEHPMVYDTMEMILESELGNATVTTISIK
                     SIKPGTLFLETFYTINSSAPKYLQLDRFIPSLPIRILMDTTGKNLSGILSYEQLNNLC
                     QPIKRHLGYPVIKQVREEIESILQRTNKIAEDQMKLFIEEAKSMMELTTSQEVNRLES
                     LQKINPAIRDEEIEFFKKKISESNYFINSSTLKLQALRVVINVS"
BASE COUNT         1093 a          671 c          630 g          968 t
ORIGIN      
        1 aataatcttt tcttttattt aaaaataaaa atttgccttc ccatgcaaat aaatcaatca
       61 atttgtcact aaaattattt gtgtaacttg tatcaaaaat tgatacaatc acgcattaaa
      121 acatacagtt cattttcaat tatttaataa tgaacgtgag ttatggataa gataggtcga
      181 tatctctaat cgttcgggct gaggaggcat ttatgccgtc tcgaagcctt atacggaatt
      241 taatgtttca tgccaaagct tcgagacgat gcttaggcat ctcctcagcc cgaacgaacc
      301 ctgcatatcg actgcttctt atataatcct tatccataat tcatgttaat gaggaagttt
      361 ctctaaatga catttcgcat tggtcaacgt tggataagta ataccgaatc acaactgggt
      421 ttaggcatta ttactgaagt agatggcaga caagtttcta tcagtttccc ggctgctggg
      481 gaggacagaa tttatgctat agataatgcg ccattaagcc gcatagtcta taaaacaggt
      541 gaagaaatac ttactaatga tcagcaaaga ataaaagtaa cttccatcca ggaacaagat
      601 ggtgttttac tgtatacagg aaatgacgac ttgggaaatg tggctcaagt tagtgagctg
      661 gcacttaatt gttttattaa acttaactcc cctcaacaaa gactcttcag cggattatta
      721 gacaaactaa atgcttttaa actcagaatt aaaacattag agcatttagg ccgattacaa
      781 cagtcaaaag caagaggcct attaggctca cgaactaacc accttatcca ccaaatctat
      841 atcgcttatg aagttgccca aagatttgct cccagagtga tgcttgccga cgaagttgga
      901 ttgggaaaaa ccattgaagc gggaatgatt ttgcattatc aactgcatac tggccgtgct
      961 tcacgcgtac tgatcattgt tccaaattca ttaattcacc aatggtttgt cgaaatgtta
     1021 aggagattca atctgcattt ctctattatt ggtccaaatc aatacgatgc tatgccaacc
     1081 acacttgatg agttggaaga taacgacaat ccaatagagc cagcatcaga agatggcaat
     1141 ctgtttgaag atgaacaact ggtattaacc agcctggatt tccttatgga aaatgaaaca
     1201 gcacgtcaac aggcattagc cgcgcaatgg gatcttctgg tagtagatga ggcgcatcat
     1261 ctctattggt cggaagactc acaaagtccc gaatacacgt tcattgaaaa attatccgct
     1321 cgaagcacag gcctgcttct tcttaccgca actcctgaac aagttggaat gaagagtcat
     1381 ttcgctcgtc ttcgtctact tgatccagca cgcttttatg atttttcagc ctttgtaaat
     1441 gaagaaaagc aataccatga aattaataaa atagttcagg atctcatgga ttataaggaa
     1501 aaaaatggcg tagatgagtt aaatccccaa ctccactctc atttaaaaca actcttgggt
     1561 aaagaagctc ctgctggcat taatgcaacg atcaaagagt tgcttgatag attcggcaca
     1621 ggtcgagtac tttttcgcaa tactcgtgca gcgattaaag gatttcccga aagaagaata
     1681 caccctgtcg cattaacatg cccagcgctt tattccgaga taatcagaga atccaactta
     1741 atccatcttt accctgaaac tttgcttaat aatgactcct ggatagaaca tgacccccga
     1801 gtgcaatggc ttgttaacaa aattgatgaa ttacgtccgg aaaaaattct ggtaatctgc
     1861 gcaaaggcaa aaacagccat tagtctggaa cagtatttaa aattaaaaac aggcattcga
     1921 agtactttat ttcatgaagg acttaccatt atagagcgtg acagagccgc cgcttatttt
     1981 gcagaacaag aaaatggtgc acaggttctt gtttgttccg agataggaag tgagggaaga
     2041 aacttccagt tttcccatca tttggttttg tttgatttgc cattaaaccc tgacctttta
     2101 gaacagcgta tcgggcgttt ggaccgtatt ggtcaaaatc atcccattga aattcatgta
     2161 ccctatttag cgggtaccgc gcaggaaaag ctatttcgct ggtatcacga aggaatcaac
     2221 attcttcaac aaagctgctc tgttggcttt tcaatttttg aagtttttga aaatcggctg
     2281 acacctgttt tatccaatcc cctacaggat actagcgagc aatcattaga gaagcttatt
     2341 gctgatactc aattgcatac ccagcatatt aaggaagccc ttaatgcagg aagagataga
     2401 ttactagaat taaactcctg taattacact atagggaaag aactcattga agcgatagaa
     2461 gcagaagaaa attgccttga acttgaaaat tacatgtcgc aagtgtatca ggagtatgga
     2521 attgatcagg aatatcattc cgagaatgcc gaaatattgc gccctggtaa ccacatgaaa
     2581 accgctcatt ttcctgggct aaaagaagac ggtatgaccg tcacttattc tcgctctaaa
     2641 gcccttgttc gggaagacat ggagttttta agttgggaac accccatggt ttacgacact
     2701 atggaaatga tcctggagtc agaacttggc aacgccacag tgactactat ttcaatcaaa
     2761 agcataaaac cgggtaccct cttcctggaa actttttata ccattaattc ttccgcccct
     2821 aaatacttac aacttgaccg atttattcct tcattgccta ttcgaatttt gatggatact
     2881 accggcaaaa atttatcagg cattttaagt tatgagcagc ttaataatct gtgccaacca
     2941 attaaacgtc atctgggtta ccctgttatt aaacaagttc gtgaagagat tgaatccatt
     3001 ctgcaacgaa ccaacaaaat cgcagaagat cagatgaaat tatttattga agaagcaaaa
     3061 tcaatgatgg agctaacgac ttctcaagaa gttaatcgac ttgaatcctt gcaaaaaatt
     3121 aatcccgcca ttcgcgatga agaaattgag ttctttaaaa agaaaatttc agagagtaat
     3181 tatttcatta atagctccac tttaaagctt caagcccttc gggttgtaat caatgtcagt
     3241 tagttaatca tcccttgaca aattgacacg agaataaaag aaattgcttg tcttacaggc
     3301 tggacgctga tttcacaaaa tcaacaacta ttagattatt aacggaaaat ttctgtttat
     3361 ag
//
LOCUS       DAXLDT010000243         3355 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXLDT010000243 DAXLDT010000000
VERSION     DAXLDT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.244"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            3..3197
                     /locus_tag="ACF2SP_002975"
     CDS             3..3197
                     /locus_tag="ACF2SP_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360735.1"
                     /translation="MHNDSATLHLSDQTIERDLIEQEESQAKQTHRVLLGNVRLLKYS
                     GSSNTEAIKELEQHCLFMDYLQLYKQEFVKDGKNTVFLIALRNLLSHSHEEQLRLMPK
                     INELFQILLRDNKESALSFYDKNKELFRYCTEIQERVTFDLLQQKMEADSKLVMTQLD
                     YFNEHLTYQENPLGIIALCRNWIGETEKFTALILWLLERKVSVEKILLTNLLQDFLKY
                     HLFTLHSKDNEVSRLYSLLSHFPEAQELVMAAQKISCGELMFQQYSLDGIFRGKNLPV
                     VSPEIPPLQFSLSKDNFIALHRTFGPAFLTAGIATATNHSEVAWLDMLRHTLNQPETL
                     KLLPDIINIIAREYSPKVLKTLAELIAESTAHQLLTLNQSCVFHLLQHKPRLLHDITE
                     ENVIEYINHLVRLDTHDQEIIYQLMALFRVLLKKNHPATKAVFEAILDNLVNHPQLLE
                     DEEFLTQLKRYPDCELLLEERCENILKQLNFCIAEQTSGLLFGKHNYYIIEDVWLRAL
                     RKFAVLQQINPQMKFSFGHKYALQARIAEVVFIHQGDLFDLDTFIDALDLPPVTSSGE
                     ISPYERTLIEILATIDNDLIRKQIIQKLETTPFNRLDWHEREYGNQTVFIKAAKKGNL
                     GLINLLEDKMKPSVLNKALRAAAKNYQWETLDHLCSLPGVELRQDEMDDLVVYLAEHG
                     RIESVKKLLKLYDYKPSTELIGTILKKAIDNDNLQVVMYFCKLPVESPKQSMLDRLFK
                     LAIQLQHWDIVEYLANSKHYSPSQTTLEKAFQQTALAMQHEAVEILGNVEKTPVRAIV
                     IERALLKASKLGHTKVVQSICALPSETLTKQAIEDALEQAAAEGHLDIVSCLCEPGTT
                     TLRQPGINSGMKIAVQAGKLSVVNYFCSMTGSNKPTPRLIDQTLVMAAKKGQTAIFIA
                     IHSNHQTPPGKHAIEQSFQLAITAGKLPILDYLCRHEGYGLNQSKVDQALISAVKSKQ
                     LEIVSYLCESLEITPSRKALRIAVSKAISSDQTGLAEYLRSRSTDKSKHTDTLVDEID
                     STSKVGKQLEANGLFKKKKRQTDREPQILFNPTI"
BASE COUNT         1106 a          672 c          632 g          945 t
ORIGIN      
        1 ggatacacaa tgatagcgca acccttcatc tcagtgatca aacgatcgaa cgtgatctta
       61 ttgaacaaga ggaatcacaa gccaaacaaa ctcatcgtgt gcttctgggt aatgtccgtc
      121 ttttaaaata tagtggttca tccaatacag aggcgattaa agaattagag cagcattgtc
      181 tttttatgga ttatctccag ttgtataaac aagagtttgt taaggatgga aaaaacacag
      241 tttttttaat tgcattaaga aacctgttat cccactctca tgaagagcaa cttcgtctca
      301 tgcctaaaat taatgagtta ttccaaatat tattacgaga caacaaagaa agtgctctat
      361 ctttttatga taaaaataag gaattattca ggtactgtac agaaattcaa gaaagggtaa
      421 cgtttgatct cctccagcaa aaaatggagg ctgacagtaa attagtaatg actcagctag
      481 actatttcaa tgagcattta acctaccagg aaaatccctt aggaatcatt gcgctatgcc
      541 gaaattggat tggtgaaaca gaaaaattca ctgctttaat actctggttg ctagaacgca
      601 aagtaagtgt tgaaaaaatt cttcttacta atctgctgca ggattttctt aaatatcatt
      661 tattcacttt gcattccaag gataatgaag tttctcgctt atactccctg ctcagccatt
      721 tccctgaagc tcaggaactc gttatggcag cccaaaagat aagctgtgga gaattaatgt
      781 tccagcaata ctctcttgac ggcatttttc ggggtaaaaa tcttcctgtt gtttcaccag
      841 aaataccccc tttgcaattt tccttaagca aagacaattt tatcgcatta catcgaactt
      901 ttggcccggc ttttttaact gcaggaattg ctactgcaac aaaccatagc gaagtagctt
      961 ggctggatat gttaaggcat actctgaatc agccagagac actcaaatta ctgcccgata
     1021 ttatcaatat tattgccaga gaatattcac cgaaagtctt aaaaaccctt gcagaactca
     1081 ttgcggagtc tactgcccat caacttctta ctcttaacca aagctgtgtt tttcatttac
     1141 tacagcataa accaagattg cttcacgata ttacagaaga aaatgttatt gagtatatca
     1201 atcatctggt tcgactggat actcatgatc aggaaatcat ttatcagtta atggctttgt
     1261 ttagggtgtt attgaagaaa aaccatccag caacgaaagc agtattcgaa gcaattctcg
     1321 ataatcttgt aaatcatccg cagctactgg aggatgaaga atttttaaca cagttaaaaa
     1381 gatatccgga ttgtgagctc cttttagagg agcgttgtga aaatatccta aagcagctta
     1441 atttttgcat tgctgaacaa acatcaggac tactctttgg caaacataac tactacatca
     1501 tagaagatgt ttggcttaga gccttaagaa aatttgcagt actccagcaa atcaacccac
     1561 agatgaagtt tagctttggg cataagtatg cccttcaagc aagaattgcc gaagttgtct
     1621 ttatccatca gggagacttg tttgatttag atacctttat cgacgcgttg gatttgcccc
     1681 ccgtcacctc atcaggagaa atcagtcctt acgaacggac tcttatcgaa attcttgcca
     1741 cgatagataa tgatctcatc agaaaacaaa tcattcaaaa gctggaaaca acccctttca
     1801 atagacttga ctggcatgaa agggaatatg gaaatcaaac tgttttcatt aaagcagcaa
     1861 aaaagggtaa tttaggctta atcaatttgc ttgaagacaa gatgaaacct tctgtgctta
     1921 acaaagcact aagagcagca gccaaaaact accaatggga aacattggat catctttgta
     1981 gtcttcctgg cgttgagtta aggcaagatg aaatggatga tcttgtggtc tatctggcag
     2041 aacatgggcg aatagaaagt gtaaaaaaac tcttgaaatt atatgattac aagccatcca
     2101 cagaattaat tggcacgatt cttaaaaaag cgatagacaa tgataattta caagtcgtca
     2161 tgtatttctg taaactgcca gtggaatcac ccaaacaatc aatgcttgat cgcctcttta
     2221 aactggcaat ccaattacag cattgggaca ttgttgagta tcttgccaac tcaaagcatt
     2281 attctccatc ccaaacgacg cttgaaaagg cttttcaaca aactgcgctg gctatgcaac
     2341 atgaagctgt agaaattctt ggcaatgtgg aaaagacccc cgtacgtgca atagtcattg
     2401 agcgggcttt actgaaagcc agtaaactgg ggcatacaaa ggttgttcaa tcgatttgtg
     2461 ccttaccttc cgagacatta accaaacaag ctattgaaga tgctctagaa caagcagcgg
     2521 ccgaaggaca tttggacatt gtctcctgcc tctgtgagcc aggaaccaca acgctaaggc
     2581 aaccagggat caattcagga atgaaaattg cggtccaggc aggcaaatta tctgtggtta
     2641 attatttctg ctcgatgaca gggagcaata aaccaactcc ccgcttaatt gatcagacat
     2701 tagtgatggc cgctaaaaag ggacaaacag ccatttttat cgccattcat tcaaaccacc
     2761 agactcctcc gggaaaacat gctattgagc aatcctttca acttgctatc actgcaggaa
     2821 aattaccaat cctagactat ttgtgccgtc atgaagggta tggtctcaat cagagcaagg
     2881 tagatcaagc attgatttca gcggtcaaat ccaaacaact ggaaattgtt agctacctat
     2941 gtgaatcact ggaaattaca cccagtcgaa aagcactaag aattgctgta agtaaagcaa
     3001 tttccagtga ccaaactggc ttggccgaat acttgcgaag ccgatcgacg gacaaatcaa
     3061 aacacacaga taccctcgtt gatgagattg attcgacatc caaggttggc aaacaattag
     3121 aagctaacgg gctatttaag aagaagaaaa ggcaaaccga cagagagcct cagatacttt
     3181 ttaatcctac tatttaaaac tattggtcag tagaggagaa tattctttct ttaatttcgt
     3241 ttgacctttt gtacctcgcg attatttttg cgcgaaatat ttttatcatt agttggtata
     3301 tacaaataat aaccatatgg taactgttca tgaggtatgt aattaaggaa tatta
//
LOCUS       DAXLDT010000244         3328 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXLDT010000244 DAXLDT010000000
VERSION     DAXLDT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.245"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            309..602
                     /locus_tag="ACF2SP_002976"
     CDS             309..602
                     /locus_tag="ACF2SP_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HFD2360736.1"
                     /translation="MIKNGIFTAVAAAITLTTSVAFAEGEMEKCSVVKDGKGLIKENK
                     ADCKGSSHSCAGQNKAGDSESWILVPKGQCDKINAGDFSAVGQDIKDKIEVSN"
     gene            617..1471
                     /locus_tag="ACF2SP_002977"
     CDS             617..1471
                     /locus_tag="ACF2SP_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFD2360737.1"
                     /translation="MTLNPLIGIGLRYPHYTQVLEERPSIGWFEVHSENFFSNGGNEI
                     KALLNISEYYPISLHGVGLSLGSSDGISKVHLDRLSNLVHQVQPKFVSEHLSWGHVGG
                     IHMPDLLPVPYTEESFNTFKRSISIAQDFLKREILIENPSSYIEYKSSRLHESYFLVE
                     LCNQTGAKILLDVNNLFISSWNHGWNTKEYIDSIPFNLVKEIHIAGHSIKELSNDSTL
                     RIDTHNNYVCDEVWALYDYTVQRFGPIPTLLEWDADIPSLNVLIEEASKCESYLSSYK
                     VNRGVANA"
     gene            1464..2243
                     /locus_tag="ACF2SP_002978"
     CDS             1464..2243
                     /locus_tag="ACF2SP_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HFD2360738.1"
                     /translation="MHELLKLQESFYRNIFKKDVDLSFISSDFSQERLDVYRQTIFEN
                     MINALRITYPGVWKLIGNECANSVAYTYCKKDKYLPKTGWLDDFGGDFPDFLSTLQQL
                     SELPYLSDYAHYEWLKHLAYGAVDSKSISPQDLMTIPEEEIDHIKLNFCPSVCIFQSK
                     YPLFDIHDTIENCSSKAITLKTEAAYGVIGCKENEIHTYWIAEDQWHFIKKLFEGAKL
                     LESVQYAQTINQNFDLTSAVAFVLQEQLVDNIIKTGGNNAQ"
     gene            2233..2730
                     /locus_tag="ACF2SP_002979"
     CDS             2233..2730
                     /locus_tag="ACF2SP_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFD2360739.1"
                     /translation="MLNNSVETSINKPFISKIIQIYLSVTKVLEKIALPVLVLFMRLW
                     MAKIFWYSGMTKISNWQSTVFLFREEYKVPFVPPEITACLATSFELACPVLLIFGFAT
                     RLATLPLLAMTAVIQFTYLDLIDHRYWAMLLALILFYGPGSLSLDHLISKKFGLLASS
                     RGDVA"
     gene            complement(2900..3085)
                     /locus_tag="ACF2SP_002980"
     CDS             complement(2900..3085)
                     /locus_tag="ACF2SP_002980"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFD2360740.1"
                     /translation="MILMLCTEANQAHWVILFILALYFILGRILHAYAFIYNKKHFKF
                     RVRGMVLTFSALFSLRC"
BASE COUNT         1039 a          595 c          623 g         1071 t
ORIGIN      
        1 ctattcaaaa taaagcaact gcaatcgaag gtagtaataa attgtattct gaaatagcgt
       61 tttttctaca aatatttagc gttgtattga ttattttaag gaaggatttt acagaataat
      121 aatagtctca acgatggatc ttaatcccaa gttactcctt aacacgaaag tttataaact
      181 tgtttaacct tgacagcgaa ttgatcggtg ctatatttaa aagataacat atcggatagt
      241 cctgaaataa tatgattgtt ttccaatacc atgttatgta actgttatat taatcaaggg
      301 gcattaaaat gataaaaaac ggaattttta cagcagttgc agcagcgata actttaacaa
      361 ctagtgttgc atttgctgaa ggtgaaatgg agaaatgtag cgttgttaaa gatggcaagg
      421 gattaattaa ggaaaacaaa gcagattgca aaggttcttc ccactcctgt gctggacaaa
      481 ataaagcagg tgattctgaa tcgtggatct tagtaccaaa aggtcaatgt gataaaataa
      541 atgcgggtga ttttagcgct gttgggcaag acattaaaga taaaatcgaa gtttcaaatt
      601 aatcgattcg tatattatga ctttgaaccc gctaataggt attggacttc ggtaccctca
      661 ttacacacaa gttctagagg agcgaccttc gattggctgg tttgaagttc atagcgaaaa
      721 tttcttctcc aacggcggaa atgagataaa agctctttta aatatttccg aatattatcc
      781 gataagcctt catggtgttg ggctatcttt gggttcatcc gatggtatct ccaaagtcca
      841 tttggatcgc ctatcgaatc ttgttcatca ggtgcagcct aaatttgttt ctgagcatct
      901 ttcatgggga catgttggtg gcatacacat gcctgattta cttccagtgc cttatacaga
      961 agaaagtttt aacaccttta agcgcagtat atcgatagca caggattttt taaaacgaga
     1021 aattcttatt gagaacccat cttcctatat agaatataag tcatccaggc tccatgaatc
     1081 ctattttttg gtggagcttt gcaatcaaac aggtgccaag attctgctcg atgtgaataa
     1141 cctattcatc tcatcctgga atcacggatg gaataccaaa gagtacattg attctattcc
     1201 atttaattta gtaaaagaaa ttcatattgc tggtcattcc ataaaagaat tgtcaaacga
     1261 tagtactctg cgtatagata ctcacaataa ttatgtgtgt gatgaggtgt gggctctata
     1321 cgattatact gttcaaagat ttggccctat accaacttta cttgaatggg atgcagacat
     1381 cccttctctc aatgttttaa ttgaggaagc ttcaaaatgc gagtcatacc tctcgtctta
     1441 caaagtcaat aggggggtag caaatgcatg aattacttaa attacaagag tccttttatc
     1501 gaaatatctt taaaaaagac gttgatttaa gttttataag ctcggatttc tcccaagaac
     1561 ggctagatgt ttatcgtcag actatttttg aaaatatgat caatgctttg aggattactt
     1621 atccaggcgt ttggaaatta atcggcaatg agtgtgccaa tagtgttgcc tatacttatt
     1681 gcaaaaagga taagtactta ccaaagacag gctggcttga tgattttggt ggagatttcc
     1741 ctgactttct ttccacatta cagcaactgt ctgagttgcc ctatttaagt gattatgccc
     1801 actatgaatg gctcaaacat ttggcgtatg gggctgtaga ctctaagtcc atttcccctc
     1861 aagatttaat gaccatcccc gaagaggaaa tagatcatat caaactgaat ttttgtcctt
     1921 cagtttgtat ttttcagtcc aaatacccgt tgtttgatat tcatgatacc attgagaatt
     1981 gtagttcaaa agcaattacc cttaagacag aagcagctta tggtgttatt ggctgtaagg
     2041 aaaacgaaat acatacctat tggattgccg aagatcagtg gcatttcata aagaaattat
     2101 ttgaaggtgc aaagctttta gagagcgtcc agtatgcaca aacaatcaat caaaattttg
     2161 acttaacttc agcagttgct tttgtgttac aggagcagct ggtcgacaat atcattaaaa
     2221 ccggaggcaa taatgctcaa taattcggta gaaacttcta taaataagcc gttcatttct
     2281 aaaattattc aaatttatct atcagttaca aaggttttgg agaaaattgc ccttcctgtg
     2341 cttgttttat tcatgcgctt atggatggct aaaatcttct ggtattctgg catgactaaa
     2401 atatcaaact ggcagtcgac cgtcttcttg tttagggaag aatataaagt tccttttgtg
     2461 cctccagaaa ttactgcttg tcttgcaacc tcttttgagc tcgcttgccc tgtacttctt
     2521 atctttgggt ttgcaacaag acttgcaaca ttgcctttac tggctatgac cgctgtcatt
     2581 cagtttacct atctcgattt aatagaccat cgttactggg ctatgctgct tgctttgatt
     2641 ttattttatg gcccgggttc actatctctt gaccacttaa tttctaagaa atttggatta
     2701 ttggcatctt cgcgtggcga tgttgcctaa atcaaatttg gtgtgcataa tgcttctgag
     2761 tataataagt ccagcgatct caataattct ttttctaata gattgcccgc tcagaaaaag
     2821 taaatacttt gaacagattt atgcagggta aaaaaataat tgttttatca cccgagttta
     2881 aaaagtatta aactaatgtt caacaccgca aggaaaaaag tgcagaaaac gtcagaacca
     2941 ttccccgcac acgaaatttg aaatgctttt tattataaat aaaagcataa gcatgaagaa
     3001 ttcttcccag aataaaatat aaggccaaga tgaatagaat tacccaatgg gcttgatttg
     3061 cttcagtgca aagcatcaat atcaatgcaa cccgtattta ggcgaacgct tacgtatatc
     3121 tgcctgtatt taaaacttat atgggacacg actcttttca cgaaagggcc tattaccttc
     3181 gtatgacgat cgatgtgttt cccgatataa agttgaaaat ggagactagg tacccaggaa
     3241 taatccctga tttgcaaggc gataacgatg aaaccaactg attttgcaga atattttact
     3301 gaatttctta ctgtttattt acccaaac
//
LOCUS       DAXLDT010000245         3261 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXLDT010000245 DAXLDT010000000
VERSION     DAXLDT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.246"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(24..1019)
                     /locus_tag="ACF2SP_002981"
     CDS             complement(24..1019)
                     /locus_tag="ACF2SP_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFD2360741.1"
                     /translation="MDPITHGVLGAACAQAALGKYDKRIPWQAGALAGMAPDLDVFIR
                     FSQEPLSLEQWHRYLTHSLMFIPIGGLVVALFLLCFSQYRKFWKVTIGASIVGYATHC
                     LLDSLTSYGTVLFWPWSDSRISWDIISIIDPVFTLPLLLGTAWSVIYQNQKAVIASLF
                     FCILFLIFNTILHNRALDGIHQYATTNNFKIHHPRALPKLASSTYWRIIAKQQDCLII
                     AEAKTSLFYPTTIRLIRQALSFNESMIPFALSSGQKSDLATFHWFTDGFLIVANKNPL
                     ILADARYTIDDNPIFSLWGIEILPGQEHINKLSLPKINEYCKSPDSERYKGDGLN"
     gene            1184..1507
                     /locus_tag="ACF2SP_002982"
     CDS             1184..1507
                     /locus_tag="ACF2SP_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360742.1"
                     /translation="MVQFFVAPGVSSSRFNIAFSNTAGNVGVRDSLNEWLTGISIKAG
                     SITKLTRNVDLLLTYQFTQYNNIAGVQMEPLSEDTLRGSYKPNVNTALIGFKVHPSDE
                     ITKDT"
     gene            complement(1820..2680)
                     /locus_tag="ACF2SP_002983"
     CDS             complement(1820..2680)
                     /locus_tag="ACF2SP_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFD2360743.1"
                     /translation="MISYLFSEVHFTHALKKVLGISVLVGVYSAIIYFWFEQDNLHSS
                     NWLSYSQSVFGIILGLLLVFRSNRAYERWWEARTLWGQLVNASRNLAIKIRILLSPNA
                     DEAKKFSELINTFCEVLAIHLRQKSNRDDFKKFLTRASLPNHVPSYVAQELYRELSEK
                     TKASKRLNLWLLDNEFSKFMDICGGCERIKNTFISLSFRVFVKHVFIIFILFLPCSLI
                     DTLGVWAIPATIIITYLIIAIEGIARNLEEPFGLTEDHLNLGSITESIRESVNEVLLG
                     QNSNEESNSI"
     gene            complement(2858..3094)
                     /locus_tag="ACF2SP_002984"
     CDS             complement(2858..3094)
                     /locus_tag="ACF2SP_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360744.1"
                     /translation="MKRVHHHPATHSHPSNATVQVSNGQHTVVVNPNPSYLPGQPPMY
                     HPNSFFTTHHHSPYYASQNQHYHQQPAPNTHQHP"
BASE COUNT         1049 a          584 c          614 g         1014 t
ORIGIN      
        1 gagtttatta attgtgattg tttctagttt agtccgtccc ccttatagcg ttcgctatct
       61 ggcgatttac aatattcatt gattttaggc aagcttaatt tattaatatg ttcttgccct
      121 ggcaatatct ctattcccca tagtgagaat atggggttgt catctatcgt atatctcgca
      181 tctgcgagga ttaagggatt tttattggca acaattagaa atccatcggt aaaccagtga
      241 aatgtagcca aatcactttt ttgtccggat gataaagcaa aaggaatcat tgattcatta
      301 aagcttaaag cctgccgaat cagtctgatt gtggttggat aaaaaagaga cgttttcgct
      361 tccgcgataa ttaaacaatc ttgttgcttg gctatgatgc gccaataagt agagcttgcc
      421 aatttaggca gggcacgagg atggtgtatt ttgaagttat tagtcgtagc gtattggtga
      481 atgccatcta aggcgcgatt atgtaatatc gtattaaaaa tcagaaataa aatgcagaaa
      541 aacaggcttg caattactgc cttttgattt tgatagatga ctgaccaggc tgttcctaac
      601 aatagaggta aggtaaatac gggatcaata atagaaataa tatcccaact aatacgacta
      661 tcgctccagg gccaaaataa aacggtgccg taagaagtca aggaatccaa taaacaatga
      721 gttgcataac caacgataga ggctccaatg gttactttcc aaaattttct atattgtgaa
      781 aaacacaata gaaacagtgc aactaccagc cctccaattg ggataaacat taaagaatgc
      841 gtaaggtaac ggtgccattg ctccaggctc aatggctctt ggctaaaacg aataaaaaca
      901 tccaaatccg gggccatccc tgctaaggca cctgcttgcc aggggattct cttgtcatat
      961 tttcctaaag ccgcttgtgc acaagcggca cctaaaacac cgtgagttat cggatccatt
     1021 ttttacttaa caggctgata atacaatgaa aatgcgcttc ttctgagaaa gccctagctt
     1081 tatttcattg aaaaaatgag tattatatga tgcaaaagaa tttttataga tattttttaa
     1141 aaaataaaag taattttttt caattttaat tgaatttaat gacatggttc aattttttgt
     1201 agctccaggc gtgtcatcga gtcgatttaa tattgctttt agtaatactg ctggaaatgt
     1261 aggggtcaga gatagtttga atgaatggct aaccgggata agtattaagg cgggttcaat
     1321 aacgaagcta actcgcaatg tagacttact tttaacctat caattcacac aatataacaa
     1381 tattgccggg gttcaaatgg aacctttatc cgaagatacg ttgagaggga gctataagcc
     1441 aaacgtaaac acagcgctaa taggctttaa agtacatcct tcagatgaaa tcacaaaaga
     1501 cacttaatct cagttaagga taagacctca attgcagtca tttgaggtct tatccttaac
     1561 tgattttaaa taatcagact gagcacgcca tatcgtggcc gcagcaaaca ggtgatttga
     1621 tttgaccgca tccgttgggg cattttgcta cttgaacttt attcccatca tttttgacta
     1681 tggtgccact aaccaacaat gcgtcacatt tactgcaggt taaatttttt acgcccatac
     1741 cacattgatt gcatttacat tccattataa tgacttcctt ttatttaagt agaaaaactt
     1801 ttattaatta taatccatac taaattgaat tggattcttc attagagttc tgtcctagca
     1861 agacttcatt gacactttct ctaatacttt ctgttattga accaaggttt aagtgatctt
     1921 ctgttaaacc aaatggttct tctaaattgc gagcaattcc ctctatggcg atgattaaat
     1981 aggtgataat aatggtagca ggtattgccc aaactccaag cgtatcgatt aggctgcaag
     2041 gtaaaaaaag aataaaaata atgaatacat gctttacaaa aacccgaaat gaaagcgaga
     2101 taaatgtatt tttaatacgt tcacaaccgc cgcatatatc cataaatttg ctaaattcgt
     2161 tatccaataa ccatagattc aatcttttcg atgctttagt cttctcagaa agttcacgat
     2221 acagttcttg tgcaacataa gagggcacat gattgggaag actcgccctt gttaaaaatt
     2281 ttttaaaatc atcccggtta cttttttgtc tcaaatggat ggccaatact tcacaaaacg
     2341 tattgatgag ttcagagaat tttttagcct catcggcatt gggtgataac aatatcctga
     2401 tttttattgc taaattacga ctggcattga ctaattgacc ccacagggtt ctcgcttccc
     2461 accaacgttc ataagcccga ttgcttctaa aaaccaaaag caaacctaaa atgatgccaa
     2521 atacgctttg cgaataggat agccagttac tggaatgcaa gttatcttgc tcaaaccaaa
     2581 aataaataat ggcgctgtaa acaccaacca aaacactaat tccaagtact tttttcagag
     2641 catgagtgaa atgtacttct gaaaataaat agctaatcat gacagtccat ttgattgttt
     2701 tgtttttcca gaataataga aaccgatctt tctataaagg gctttgtcaa aaaaacgtga
     2761 gagcaaaaca agtaataacc agagaagcca gttattactt atttttcagt ctaaactctt
     2821 aaagagtaat agtctgtttg ttaacagcta ccaccattta tgggtgctga tgagtattgg
     2881 gtgctggttg ctggtgataa tgttgattct gagaagcgta atagggtgag tgatgatgcg
     2941 ttgtaaaaaa actattgggg tggtacatgg gcggttggcc tggaagataa gacgggtttg
     3001 gattaacaac tacagtgtgt tgtccattgc tgacttgaac agtagcattt gatgggtgag
     3061 agtgtgttgc agggtgatga tgtactcgtt tcatagatta ttatccttat aaaaatacaa
     3121 ctgcatgaca tacataaaac tgactttact caggttattt cagaatagcc tgagtccgag
     3181 attgtaatgt agatagttaa acaagtacag atagtattaa aaatttacta ttttcgcttt
     3241 cttggctttc ttcacctcaa t
//
LOCUS       DAXLDT010000246         3226 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXLDT010000246 DAXLDT010000000
VERSION     DAXLDT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.247"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            67..624
                     /locus_tag="ACF2SP_002985"
     CDS             67..624
                     /locus_tag="ACF2SP_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360745.1"
                     /translation="MNTRIICFALILCNFIPPVFSADCNIAYMEEEKLLAMDYDSFDQ
                     LEVGWRKYAKLGCYYEIGALIDKYLDKNRNSLADWQTIGITWHAGQMYAFGNEYGIAE
                     NRFIHSINPNEPDNTPILWNDYVYATIAFLNNDVAELKFRRDKIAAGPILNGTKPNLT
                     VVDNLILYIGQPYSMAYSGNREWDS"
     gene            975..1142
                     /locus_tag="ACF2SP_002986"
     CDS             975..1142
                     /locus_tag="ACF2SP_002986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360746.1"
                     /translation="MQPGRFIAMTAIYTIMLLSKSTIAGTAGEAALASSHWNGGVNLQ
                     VVSKYPCFLFP"
     gene            1535..2122
                     /locus_tag="ACF2SP_002987"
     CDS             1535..2122
                     /locus_tag="ACF2SP_002987"
                     /inference="COORDINATES: protein motif:HMM:NF017003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HFD2360747.1"
                     /translation="MSQQYNAFETQEPGATLDIRMNPPVPQFITAHKKDLLSGHNFWG
                     WGPVIMIEDTIPLGQSGFSLVGMARGSAVYGPAKQQASKTLLVRGTQNGNEILNENAF
                     VQRKNRHQTVLEIIDAELGVAYSKKWGKGELNGQLSVVAQEWIGGGSASKSATTGAGF
                     GLPGSGMTGAPQVAGVTNESNFGFVGGAFKLGYLY"
     gene            2510..2959
                     /locus_tag="ACF2SP_002988"
     CDS             2510..2959
                     /locus_tag="ACF2SP_002988"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFD2360748.1"
                     /translation="MMMSFTGEGWGEGMSRMVIDDEMWFRLEKLLPKPKGRHGEDDRL
                     FMEAICWMLRTGAAWRDLPLDYGNRKSVYNRYNNWSKKGYITAILAELKKDGDHEWHM
                     LDGSIIKVHQDAMRSSYDRSAEAIGSSVGGLSTKIHAMTHWVNSSIY"
BASE COUNT         1008 a          544 c          696 g          978 t
ORIGIN      
        1 gccaagtgta tacggctata tttgagaagg ccacactatt aattgctcaa acacgtatag
       61 aaaattatga atacaagaat tatttgtttt gccttaatat tatgcaattt tattccaccc
      121 gtcttttctg cagattgtaa tattgcatat atggaagaag aaaaactctt agctatggat
      181 tatgacagct tcgatcaact tgaagttgga tggcgaaaat atgctaagtt aggttgttat
      241 tatgaaatag gtgctttgat tgataagtat ctcgataaga atagaaatag ccttgcagat
      301 tggcaaacaa ttggaatcac ttggcatgca ggacaaatgt atgcatttgg taatgagtat
      361 ggaatagcag aaaatagatt tattcattcc atcaacccaa acgaaccaga taatacccca
      421 atcctctgga atgattatgt gtatgcaacg attgcatttc ttaataatga tgtggctgaa
      481 ttaaaattcc gtcgagataa aattgctgct ggtcctattt taaatgggac aaaacctaac
      541 ctaaccgttg ttgataatct aatcttatat attggccaac cctattcgat ggcatatagc
      601 ggtaacaggg agtgggattc ttaaaagaat aaattttcaa gctcttgtaa tataagataa
      661 aagtcgagat tttgcttaag aattttaatc cttacaacaa ggatagtcga gtgaggtggt
      721 caagccagca tatttaacag attaggatta taaacgtatg agacatatta aaaatgaacc
      781 tatccaaaat cagttgatat cgaaataatt tagaaacaat tcataagatt atatttgcct
      841 catcatttga gatgaagtaa catctctggc gtttttaact ttttagttaa cctctcagac
      901 ctccctcatt gtttgatttg tgctgtgcaa atggggaagt ggatgtaagc aatttgacaa
      961 ggagatattg gtctatgcaa cctggtcgtt ttatagctat gacagcaatt tatacaatca
     1021 tgttgttatc aaagtccact atagctggga cagcagggga ggctgcctta gcatcaagcc
     1081 attggaatgg cggagttaat ttgcaagtag tttcaaaata cccctgcttt ttatttcctt
     1141 agtgcaagaa gagttgctgc tggtgatgca aattttacag taactgatat ccaatcccaa
     1201 aaggatgtat ctcatgatgt tgatattgcc ccagaatttt ttctagggta tgtttcggaa
     1261 tcaggcctcg gatttcggac tcgttattgg ttctttgatc aaaagaccag gcaaactgct
     1321 gcagcccccc agacttcggt taccgatttg actttatcct ttcctatttc ttcagctgct
     1381 ccaattggtt tagatattga tacacgggat aacaatttac gtgatagtat gactgttgaa
     1441 ataaaaactc aatactcaga tttgggatgg tataattacc aagaattatg aagttcaaaa
     1501 ctcagtcatt gttcttgggg cacgatatgc gtatatgtcg cagcaatata atgcattcga
     1561 aactcaagaa ccaggcgcaa cattagatat tcgtatgaat cctcctgtgc ctcaatttat
     1621 aacggcacat aaaaaagatc ttttgtccgg gcataacttt tggggttggg gacctgttat
     1681 tatgattgag gatacaattc ctttggggca aagcggtttt tctttggttg gcatggccag
     1741 aggctcagct gtttatggtc cagcaaaaca acaagctagt aaaaccttat tggtacgagg
     1801 cacacaaaat gggaatgaga ttttaaatga aaatgctttt gtgcaaagaa agaataggca
     1861 ccaaactgtt ttagaaatca tagatgcaga acttggggtt gcctacagta aaaaatgggg
     1921 taaaggagag ctaaacggcc agctttcagt tgtagcgcaa gaatggattg gcggtggaag
     1981 cgcctcaaaa tcagcaacaa ccggcgctgg ctttggtttg ccaggaagcg gcatgactgg
     2041 tgctcctcaa gttgcaggag tgacaaatga aagcaacttt ggttttgttg gaggtgcatt
     2101 taagcttgga tatttatatt aatttttaat tgagtccaaa ctcatgagag ttttctcttt
     2161 acaatactat gcagaaaact ctctcacatt tttcactgta atttttctac cataaaacaa
     2221 ttcttaaata attggatgac taaccagaca attaatttgg gttgataaat ttgcaagaac
     2281 tacctttaaa tttaagaata cctgtcgggg ataagacaaa aagcagcctt aacaaggaag
     2341 gtttttccag ttatgtttca aaatccagct attccataac tgtctttaat agatttaatt
     2401 taagcaacgt ccctgaagtt ttttagacaa gatttaatct tgaattgagc acggactttt
     2461 caatttcact tcgatctgct ccctctcccg cgccaggagc tttaatagta tgatgatgtc
     2521 tttcacggga gagggttggg gagagggaat gtcacgtatg gtcattgatg acgagatgtg
     2581 gtttcgttta gaaaaattac ttccaaagcc taaaggccgg catggtgaag atgatcggct
     2641 gtttatggag gcaatatgtt ggatgcttcg tactggtgct gcatggcgtg acttgccgct
     2701 ggactatggc aacaggaaaa gtgtttataa tcgttataat aactggtcga aaaagggtta
     2761 tatcaccgca atattggcgg aattaaaaaa agatggcgat cacgaatggc acatgcttga
     2821 tggctcgatt ataaaagtgc atcaagatgc aatgagaagc agctacgata gaagtgcaga
     2881 ggcaattggc tctagcgttg gtgggcttag cacaaaaatt catgccatga ctcattgggt
     2941 caactcgtca atatactgat aacacctgga caagtacatg agagtcaagt ggctcatgaa
     3001 gtttgggcgc atgagtcttg tgagttcttt ttggcagata aggcttagga tattgatggt
     3061 tttcgaaaag cattaaccga tgatgggata agagctgtta tcccaagcaa aaagaaccgc
     3121 ttgaatccaa cgaaccacga tcgtgatatc tacaaagaaa ggaatattgt tgagcgcttt
     3181 tttcagtaga ttaagcgttt tagacggatt gctacaagat atgata
//
LOCUS       DAXLDT010000247         3177 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXLDT010000247 DAXLDT010000000
VERSION     DAXLDT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.248"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1664
                     /locus_tag="ACF2SP_002989"
     CDS             <1..1664
                     /locus_tag="ACF2SP_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFD2360749.1"
                     /translation="LTGLVPFNKAFSGFSNPAVITVGCVMILTYAITQSGVLNNFFIK
                     LKKSSKKTSFQVGLYSGISAFLSAFMNNVGALGIMMPIAIHAFTEIKKSPSIILMPIA
                     FCSVLGGVITAIGTPPNLLISNFRNQVLGTPYNMFDFTPVGLPVALMGVLFVSLVGWK
                     LVPVRAKYSKSGDSFQISDYMTEVKVPETSSMCEKTVKEFLEMTKANFELIAVAHEGK
                     KKFAYTDDTIIHCNDILIIEASPEDLKNILDSNGLILAGNKPLTSKELCDSDIITMEA
                     VVLPDSSIEGQSASMMRFRSRHKINLLAISREELSFRKKLIDIRFSAGDVVLLQGNAA
                     TLRETIVDLGFLPLAERDINVHLTQKKFLPIICFTLSIILASLQILPLDFSFMIAVLA
                     LILLKAIPTHSLYRTIDWSVLLLLAALIPVGEALQTTGAADLIANGFMKIAGQYSPIF
                     ALVAILVITMTLSDLLNNAATALIMAPIAIKIAQSAHVNVDSFLMAVAIGASCSFLTP
                     IAHQNNTMVMGPGRYRFSDYFRLGLPLELLVVVVTIPTILWVWPL"
     gene            complement(1767..2222)
                     /locus_tag="ACF2SP_002990"
     CDS             complement(1767..2222)
                     /locus_tag="ACF2SP_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HFD2360750.1"
                     /translation="MKQIKGMLLSIISLSFLPFLVFASDASISLVGVQGYDLVSYHQK
                     NGPVRGSGNHTAYHNGVAYLFATDENKKVFQANPEKYLPAYGGYCAFAVSVGRKVVSD
                     PLAWKIVDGILYLNLNKQVQEFWSKDIPGNIKKGNIQWEKIKDIDPQKI"
     gene            complement(2270..>3177)
                     /locus_tag="ACF2SP_002991"
     CDS             complement(2270..>3177)
                     /locus_tag="ACF2SP_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg1273 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360751.1"
                     /translation="LLKSPSFNHVGSEFQPMVSKRDVNSISITLPIKHNVHRNHDGNL
                     PGDSISVVDSSNQQDHILYLQLCFTHEEGKWYLFLKCLTTLDVFNYDFAPLLKILHKP
                     DIRSYPMSLGCPGVIDRTELVNMDRTIYVRQFIIHGPDDEAKILQDIHDFNMNCLRRF
                     EELTYGRDNSAYRNSQALGRILHKIITAYQEVTVNEYDIALNRLKIVFQDQVFQNDPQ
                     ICLSLTHIVNALIVLSQKKELNNKGVEIDEHQLHLILKELAKHEGKKFKNNLWFPIGT
                     VDAIRQELEDLFISKQTISTAPIMC"
BASE COUNT          981 a          606 c          526 g         1064 t
ORIGIN      
        1 tattgaccgg ccttgttccc tttaacaagg cattttcagg attcagtaac ccggcagtaa
       61 taacagtcgg ctgtgtcatg atcttaactt atgccatcac ccaatcaggc gtattaaata
      121 atttctttat aaaattaaaa aaatcttcaa aaaaaacatc atttcaagtc ggtctttata
      181 gtggcatcag tgcatttctg tctgctttta tgaataatgt aggtgcatta gggataatga
      241 tgccaatcgc cattcatgct tttacagaaa ttaaaaaatc accatctatc atcttaatgc
      301 ctattgcctt ttgctcagtt ttgggaggag taatcactgc cataggtacc cctcccaacc
      361 tattaatttc caacttccgc aatcaagtcc tgggcacccc atacaatatg ttcgatttta
      421 ctcccgttgg gcttccggta gcattgatgg gtgttctctt tgtttcattg gttggatgga
      481 aattagttcc agtgcgggcc aagtatagta aatcgggaga ttcattccag atttctgatt
      541 acatgactga agtaaaagtt cctgaaacct cttctatgtg cgaaaagact gttaaagagt
      601 ttctcgagat gactaaagcg aactttgaac tgatagcagt tgcgcatgaa ggtaaaaaaa
      661 aatttgcata cactgatgat acgatcattc attgcaacga tattttaatt attgaagcgt
      721 ctcctgaaga tttgaaaaac attttggatt caaatggtct aatcctcgct ggcaacaaac
      781 cattaacctc taaagagctc tgcgacagtg atatcatcac tatggaagct gttgtattac
      841 cggattccag tattgaagga caatccgctt ccatgatgag atttcgttca cgtcataaga
      901 ttaatttgtt agctatttct agagaagaat tatcgtttag aaaaaaatta attgatattc
      961 gtttctcagc aggagatgtg gtactactgc agggaaatgc cgcaacgctt cgcgaaacaa
     1021 ttgtcgacct ggggttcttg ccccttgcag aaagagatat taatgtccat ttgacacaaa
     1081 aaaaatttct accaatcatt tgttttacac tatccatcat tttggcatct ctgcaaatac
     1141 taccactcga cttttctttt atgatagcag tgcttgcgct cattctatta aaagcaatac
     1201 caacacacag tttgtatcgt actattgatt ggtcggtact attattatta gctgctctta
     1261 tccctgttgg agaagcattg caaacgactg gtgctgcaga tttgattgca aatggattta
     1321 tgaaaattgc tgggcaatat tcccctatct ttgccttggt tgcaattctg gttatcacta
     1381 tgactttatc tgatttatta aacaatgcag caacagcatt aattatggct cccattgcaa
     1441 tcaagattgc tcagtcagcg catgttaatg tggattcttt cttaatggca gtggctattg
     1501 gtgcttcgtg ttccttcctg actccaattg cccatcaaaa caacaccatg gttatgggtc
     1561 caggaagata ccgattttct gattatttcc gtttgggatt accattagaa ctccttgttg
     1621 tagtcgttac tattccaact attttatggg tatggccatt ataaaaatag aaattaatat
     1681 gcattaaacc tgcaccataa tcgaaaaaac actatggtac aggttagaga tatttgaata
     1741 gacgcaaatg gtatattgca attacattaa attttttgtg gatcaatatc tttaattttt
     1801 tcccattgaa tattaccttt ttttatgttt ccaggaatat cttttgacca aaattcttgt
     1861 acctgtttat taagattgag atataaaatg ccatcaacaa ttttccatgc taatggatca
     1921 ctcactactt tccgccctac tgaaactgca aaagcacaat aaccaccata tgctggcaaa
     1981 tatttttcag gattagcctg aaacactttc ttgttttcat cagtagcaaa taggtaagct
     2041 actccattat gataagcagt gtgattacca ctaccacgga cagggccatt tttctgatga
     2101 taagaaacca aatcatagcc ttgaacacca accagactaa tcgacgcatc agaagcaaac
     2161 accagaaacg gcaagaatga taaagatatt attgataata gcattccttt aatttgtttc
     2221 atttttatcg tccttaatta aattgtaact ggtagtactt aaatcactat taacacatta
     2281 taggtgctgt agatatagtt tgctttgaaa taaacagatc ttctaactct tgtctaattg
     2341 catcaacagt ccctatagga aaccatagat tatttttaaa ttttttacct tcatgttttg
     2401 caagttcttt taaaatgaga tgtaattggt gttcatcaat ttccactcct ttgttattaa
     2461 gttccttttt ttgacttaaa acaatcaatg cattgactat gtgggtcaaa gacaaacaaa
     2521 tttgtggatc attttgaaat acttgatctt gaaacacaat cttaagccgg ttcaatgcta
     2581 tatcatattc atttacagtt acttcttgat aagcagttat aattttatgc aaaattctcc
     2641 ccagagcttg agagtttcta taagcactat tatctctacc gtaggttaac tcttcaaacc
     2701 gcctcaaaca gttcatattg aaatcatgaa tatcttgcaa gattttagcc tcatcatcag
     2761 gcccatgtat aataaactgc ctgacataaa ttgttctatc catgttaacg agttctgtcc
     2821 tatcaataac cccagggcaa cccaaactca tgggataact tcttatgtct ggtttatgta
     2881 gtattttcaa taatggtgca aaatcataat taaaaacatc cagagttgtt aaacacttta
     2941 aaaataaata ccactttcct tcttcatgag taaagcaaag ctgtaaatat aaaatatgat
     3001 cctgttgatt agatgagtcc acgactgata ttgaatcacc tggcaaatta ccatcatgat
     3061 ttcgatgaac attgtgttta attggtaatg taatcgagat tgagtttaca tccctcttgg
     3121 ataccatggg ttgaaattct gaaccgacat gattgaaaga ggggcttttt aataaaa
//
LOCUS       DAXLDT010000248         3158 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXLDT010000248 DAXLDT010000000
VERSION     DAXLDT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.249"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..233
                     /locus_tag="ACF2SP_002992"
     CDS             <1..233
                     /locus_tag="ACF2SP_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360752.1"
                     /translation="QTRKNYQYHFKLKQVYRSGHLAVARITWYLQIFEQGKLISETQD
                     EGLDVFLKDARNQWKIINFIGYPVPGNLDEKH"
     gene            318..596
                     /locus_tag="ACF2SP_002993"
     CDS             318..596
                     /locus_tag="ACF2SP_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360753.1"
                     /translation="MSISMITEIETLLKKAGFTYVGNGRGLGEFEKYENHLYHKNVYG
                     SIQQIQLAMDRENDTVRPVFSKNVPIELRDSIYELMKNPSEKNSLTFN"
     gene            779..1459
                     /locus_tag="ACF2SP_002994"
     CDS             779..1459
                     /locus_tag="ACF2SP_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFD2360754.1"
                     /translation="MKPIAHIADKEIISIPVKECGEELIDLKNQNIIVYGPPPETPLT
                     ANDYTKIRKTVYQKLCLAQNDLPYGWRFRLYEGLRSLTVQEILFNQQRDNLRQSKQYD
                     SYEDLFNETCRLVSPVVHLDGTANIPPHSTGAAIDIELIDSYGNLVNLGMAAKDWLIV
                     DPDLSETYSKLISEEAMQNRILMLKIMAKHGFVNYPREWWHFSYGDRLWAYMTEHSTA
                     IYGAIINN"
     gene            1542..2270
                     /locus_tag="ACF2SP_002995"
     CDS             1542..2270
                     /locus_tag="ACF2SP_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFD2360755.1"
                     /translation="MTPSISLITSYSLLPAILMLIGGGTASLYRPGGSITSATQHFAA
                     GVVFAAVAKELLPKIGAYHDPVALIIGFSVGILGMLFLKWLANRLEDLEQEKTGLSWG
                     LLTAVGIDLLIDGILIGVAFLAGERGGILIAIALAIEIFFLGLSTTATLGARQVSVPI
                     CLLTSLILAILIPVGAALGASLLIHLPLSITNGILSFGVAALLYLVTEELLLEAHEVR
                     ETPYITLCFFIGFLLILLLEGLAA"
     gene            complement(2350..>3158)
                     /locus_tag="ACF2SP_002996"
     CDS             complement(2350..>3158)
                     /locus_tag="ACF2SP_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HFD2360756.1"
                     /translation="NQHPVQRQSLSFFNYTKLLSSEHGGYCYQSAGLLFDALMQLGFK
                     TEYCAARVLVGEPVNSPKVLAIPATHLVLVVTLGDKKFLLDPGLGASSPRLPILITEK
                     DESILQGKEEFKFYKYDGAHVLETKTSQGWFRLMQTDLIPIDLKTVEMNLLKLERYPD
                     TISIRDNKTVVGSITEYGRKSLVWDAQSNQLKFSKHDGDEVIQKTLTSFEEGVQILVK
                     EFDIDHISAQELKTYCTETNLPQPIKSWTVDFPLDQAELKRMEENLIFRL"
BASE COUNT          981 a          609 c          590 g          978 t
ORIGIN      
        1 agcaaacaag gaaaaattac caatatcatt ttaaattaaa acaagtctat cgctcaggcc
       61 atctcgcagt tgcaagaatt acctggtact tgcaaatatt tgagcaagga aaactcattt
      121 ctgagacgca agatgaagga ttagatgtct ttctaaaaga tgcgcgaaat caatggaaaa
      181 ttatcaattt cataggatac cctgtccctg gtaatctcga tgaaaaacac taaaacaagg
      241 tggcataata aattgtaata tgctccttga ttgatcttgt tgtattttat caatataatc
      301 gccaccctat ttatttgatg agtattagca tgatcactga aattgaaaca ttactaaaaa
      361 aggcgggatt tacttatgta gggaatggca gaggcttggg ggaatttgaa aaatatgaaa
      421 atcacctcta ccataaaaat gtttatggct caatacaaca gatccaattg gctatggaca
      481 gagagaatga cacggtgaga ccagtgttct caaaaaatgt tccaatagag ctacgtgaca
      541 gcatttatga actcatgaaa aacccatctg aaaaaaattc gttaacattt aattgacatt
      601 agcgtttgag ccataatttc cttaaatggc caactgcaga ttgttaggtt ttatcagaaa
      661 aatattaaaa aattaaagca taaggcctaa cctgcttttt tgttagcatg atataattaa
      721 tgtgcagata acttgtcgaa tccatgcaat aaaattttgc ttgttttgcg aaaaaataat
      781 gaaaccaata gcacatatcg cagataaaga aataatatct atccctgtta aagaatgcgg
      841 cgaagagctc attgatctta aaaaccaaaa tattattgtt tatggacccc ctccagaaac
      901 ccctttaact gcaaatgact ataccaaaat acgtaaaact gtatatcaaa aattatgctt
      961 ggcacaaaat gatctaccct atggctggcg ctttagatta tatgaaggat tgcgtagtct
     1021 gactgttcag gaaattctgt ttaaccaaca acgtgataac ctgcgtcaaa gcaaacagta
     1081 cgattcctat gaggatttat ttaatgaaac ctgtcgtctg gtttctccag tagtccattt
     1141 ggatgggaca gctaatatac caccgcatag caccggggcc gcaatcgata ttgaactcat
     1201 tgatagctat ggcaatctgg ttaatcttgg aatggctgca aaggactggt taattgttga
     1261 tcctgattta agtgaaacct attcaaaact tatttctgaa gaagctatgc aaaatcgtat
     1321 tctgatgctg aaaataatgg ctaagcatgg ttttgttaat taccccagag aatggtggca
     1381 tttttcctat ggagatcgcc tatgggccta tatgacagaa cattctactg ccatttacgg
     1441 tgctatcata aataattaag ccaatgtagc aatgcctgct attcttgaac tgggttaatt
     1501 atttgattat tctgtcaata ttaccttaaa gaatatctct tatgacccca tctatttcgc
     1561 ttattacctc ctattcttta ttacccgcca tattgatgtt aatcggtggg ggcaccgcga
     1621 gcctttaccg tccaggtggt tcaataacca gcgccaccca acattttgca gcaggtgttg
     1681 tttttgctgc tgtagcaaaa gaactgctac ccaaaatcgg agcataccat gatcctgttg
     1741 ctcttattat cggcttttca gtaggcattc tgggcatgtt attcctaaaa tggttggcta
     1801 acaggctcga agatcttgaa caagaaaaaa caggtctatc ctggggatta ttaaccgcag
     1861 ttggaattga tttattaata gacggtattt taattggggt tgcttttttg gctggtgaac
     1921 gaggaggaat tcttatcgct attgcccttg ccattgaaat tttctttttg ggattatcga
     1981 caactgccac tttaggagca aggcaggtga gcgtaccaat ttgtctgtta accagcctca
     2041 ttttggctat attgatccct gtgggagctg ctctgggcgc cagtttatta attcatttac
     2101 cgctatcgat taccaatgga attttatcat ttggtgttgc ggcattactt tatctggtca
     2161 ctgaggagtt attgcttgaa gcccatgaag tgcgtgaaac cccttatatt actttatgtt
     2221 ttttcattgg atttttgctg attttattat tagaagggct ggcagcataa atacaagcag
     2281 gccttttaat aacaaagata agttctgatg ggctgaatct gcctggaaaa gccaacacaa
     2341 accactgccc tatagtctaa aaattaaatt ttcttccatt ctttttaact ccgcttgatc
     2401 aagggggaag tccacagtcc aagattttat tggctgggga agatttgttt cagtacagta
     2461 agttttcaac tcttgcgctg aaatatgatc gatatcaaac tccttaacaa gtatttgcac
     2521 tccctcctca aaactggtta aagttttttg aatgacttca tcaccatcat gttttgaaaa
     2581 tttcaattga tttgattgag cgtcccaaac caacgattta cgtccatatt ccgtaataga
     2641 tccaacaacc gttttattgt ctcgaataga aattgtatcc ggataacgtt ccaatttgag
     2701 taagttcatc tctaccgttt taagatctat ggggatcaaa tcagtctgca tcaagcgaaa
     2761 ccacccttga cttgtttttg tctccagaac atgcgcacca tcatatttat aaaatttaaa
     2821 ctcttcttta ccttgtaaaa tggactcgtc tttttctgtt atcaatattg ggagccttgg
     2881 agaagaagcg cccaatcccg gatccaaaag aaattttttg tctcccagag tcacgactaa
     2941 aacaagatga gtagcaggta tcgcaagtac cttaggggaa ttcacaggtt cgccaacaag
     3001 aacacgcgcc gcacaatatt ccgtcttaaa tcccaattgc attagggcat caaacaacag
     3061 acccgcactt tgataacaat accccccatg ctctgaagaa agcagctttg tataattaaa
     3121 aaaagacaag gattgtcttt ggacaggatg ttgattag
//
LOCUS       DAXLDT010000249         3155 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXLDT010000249 DAXLDT010000000
VERSION     DAXLDT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.250"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1405
                     /locus_tag="ACF2SP_002997"
     CDS             <1..1405
                     /locus_tag="ACF2SP_002997"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFD2360757.1"
                     /translation="WSANQFGVTNDSILLYTKGKDYIFNPSFSKDDENTKKYILERFK
                     FDDGDGRKYMKSPLVNPLNRPNLRYEFHGVKPPKTGWLYSMERMEEMFANNELVMPED
                     KNARIYRKIYEDTYQGQMIQNIWLDIPIVNPMAKERVNYPTQKPIALLERIITTSSNT
                     GDLIADFFCGSGTAGLAAEKLGRRWIMVDLGRFAIHTSRKRLLDINSTPFIVQNLGKY
                     ERQHWVKMNGQYTGYLKFILELYGAGVTEELVRQFRQAFKTLHGKKGDVYIHIGNVDA
                     PVTLLEIREALQECKENNISKLVVLGWEWQLGLHELIYEEAHPYGVKLRILQIPREVM
                     ELSSNDRKKHDIQFFELACLETETKIVNKNVIVTLTDFIIPHPDLLPIEVREKISNWS
                     DYIDYWSIDWMSNQRSYKNKTIFHSMDQQYRTRENSNLNLSMSHKYDNSGNYQILVKV
                     IDIFGNDTTKMVEVKVS"
     gene            1402..>3155
                     /locus_tag="ACF2SP_002998"
     CDS             1402..>3155
                     /locus_tag="ACF2SP_002998"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFD2360758.1"
                     /translation="MSLNNTVSGLPIPNALHESVKIWRNDGYKNVSNTTKRLLEWWFL
                     EEHELDNGTPFSFWEGQREAIEHLIYCYEVLQARSLYELVRQLDVKIFIDPSEDKWTK
                     YAFKMATGSGKTIVMAMVIVWSYFNAQKEDKNKFSQNFVLIASNLIVLDRLLGDSVNP
                     TFFEGGIFRQYPFIPSEWISDFHINVVSEDELPVHSKPGLIYLINWQKFIERDLKLED
                     NPVQVVLGPKPPSDLQRKKKLKDILANLKNILVINDEAHHVWDEELIWYKAIDSLHSH
                     SPLMCQLDFSATPKDQNGNLFSHIIMDYNLGRAIEENIVKRPKIAELRNVPEIASQKA
                     SERYKVQIDAGVDQWRKIWEVMIKAGKKPVLFIMAEDNKAADDVANYLENFSDLTHHI
                     LTIHSARAKGQIADKDLDMARKAAREIDSFANPYRAIVSVLMLREGWDVRNVCIVVPL
                     RSYSSKAKILPEQTLGRGLRRMWPEQTDLEEQLIVIEHPQFHNLIEEAFKEQGVKAKF
                     YSLDDVFVSPELIQIDNEKLQYDIEIPILIGGLTHSITGLERLDFNCFDKKVFEYDSL
                     IPNEIILQKVDMLTKKLES"
BASE COUNT         1091 a          469 c          631 g          964 t
ORIGIN      
        1 ttggtcagca aatcaatttg gagtaactaa cgattcaata ttactttata ccaaaggcaa
       61 agattatatt tttaatccat ccttctcaaa agatgacgaa aataccaaaa aatacatttt
      121 agaaagattc aaatttgatg atggtgatgg tagaaaatat atgaaatccc ctttagttaa
      181 tccattaaat cggccaaatt tacgttatga gtttcatgga gttaagccac caaaaacagg
      241 ttggctatat tcaatggagc gaatggagga gatgtttgct aataatgagt tagttatgcc
      301 agaagataaa aatgccagaa tttacagaaa aatatatgaa gatacgtatc aggggcaaat
      361 gattcaaaat atatggttag atattccgat tgtaaaccct atggcaaaag aacgagtaaa
      421 ttatcctact caaaaaccta ttgcattatt agagaggata ataacaactt ctagtaatac
      481 tggagatctt attgctgatt tcttttgcgg atcaggtaca gccggcttgg cagcagaaaa
      541 attggggagg cggtggatta tggtagatct aggtcggttc gctatccata cttctcgtaa
      601 acgcttatta gatatcaact caaccccatt tatcgtgcaa aacctcggaa aatatgagcg
      661 tcagcattgg gtaaaaatga atggacaata cacaggatat ctgaagttca ttctcgaact
      721 ttatggtgca ggcgttacag aggagcttgt tcggcagttt agacaagcgt ttaaaacttt
      781 gcatggtaaa aaaggagatg tatatattca tattggcaat gtagatgcac cagtaacact
      841 cttagagatt agagaagctt tacaggagtg taaagaaaat aatatatcca aacttgttgt
      901 tttaggttgg gaatggcaat tggggctaca tgagcttatt tatgaggagg cccatccata
      961 tggtgtaaag ttgagaattt tgcaaattcc gcgtgaagtt atggagttat caagcaatga
     1021 tcgaaagaaa catgatattc aattttttga actagcatgt cttgaaacag aaacgaaaat
     1081 tgtaaataaa aatgtcatag ttacattaac agatttcatt attccacacc cagatttatt
     1141 acccatagag gttagagaga agattagtaa ttggagtgat tatattgatt attggtcgat
     1201 tgattggatg tctaatcaaa gaagctataa gaacaagact atttttcaca gcatggatca
     1261 gcaatatcgt accagagaaa actctaattt aaatctatct atgagccata agtatgacaa
     1321 ctctgggaat tatcaaattt tagtcaaagt tattgatatt tttgggaacg acactaccaa
     1381 aatggtggag gtgaaagtgt catgagctta aataatacag tttcaggcct tccaattcct
     1441 aatgcattac atgagtctgt taagatttgg cgcaatgacg gttataaaaa tgttagcaat
     1501 acaacgaaac gattactgga atggtggttt ttagaggagc atgagttaga taatggtact
     1561 ccttttagct tttgggaggg acaaagagag gcgatcgaac acttgatata ttgttatgaa
     1621 gtattacaag ccagaagctt gtatgagcta gtaagacaac tcgacgttaa aattttcatt
     1681 gatcctagtg aagacaagtg gacaaaatac gcttttaaga tggcaactgg ttccggcaaa
     1741 actattgtga tggcaatggt tattgtttgg tcatatttta atgctcaaaa agaagataag
     1801 aataagttct cacaaaattt tgtgttgatt gcttctaact taattgttct ggatcgattg
     1861 cttggtgact ctgttaatcc aacatttttt gaaggaggga tttttaggca ataccctttc
     1921 ataccttctg aatggatatc agattttcat attaatgttg taagtgaaga tgagctgcca
     1981 gttcatagca agccaggtct aatttatctt ataaattggc agaaatttat tgaacgtgat
     2041 ctaaagttag aagacaatcc agtccaagta gttttaggcc ctaagccccc aagtgatctt
     2101 cagcgtaaaa aaaaattgaa agacatattg gctaatttaa aaaatatcct cgtaattaat
     2161 gatgaggcgc atcatgtttg ggacgaagaa ttaatctggt ataaggctat agattctttg
     2221 catagccata gtcctttaat gtgtcagtta gatttttctg caactcctaa agatcaaaat
     2281 ggaaatttat tttctcacat tattatggat tacaatttag gtcgagctat tgaagaaaat
     2341 attgtaaagc gacctaaaat agcagaactt agaaacgttc cggaaattgc tagtcaaaaa
     2401 gcttctgaaa gatataaagt tcaaattgat gctggtgtag atcaatggag aaaaatttgg
     2461 gaagtcatga taaaagcagg taaaaaacct gttttattta ttatggctga agataacaaa
     2521 gctgctgatg atgtggccaa ttatttagaa aatttttctg atttaactca tcacatacta
     2581 acgatccatt ctgcacgtgc aaaaggtcaa attgctgaca aagaccttga tatggctcgc
     2641 aaagctgcac gagaaattga ttcattcgct aatccttatc gtgccatagt atcagttttg
     2701 atgcttcgtg aaggctggga tgtccgtaat gtatgtattg tagtaccttt acgttcttat
     2761 agctcaaaag caaaaatttt acccgagcaa actctaggcc gaggattaag acgaatgtgg
     2821 ccagagcaaa ccgatttgga agaacaactg atcgtgattg agcaccccca attccataat
     2881 ttaattgaag aagcgttcaa ggagcaaggt gtaaaagcaa aattttattc tctggatgat
     2941 gtatttgtat caccggaatt gattcaaatt gataatgaaa aattacaata tgatattgaa
     3001 ataccaatct taatcggtgg actaacgcat agtattactg gtcttgaaag gctagatttt
     3061 aattgttttg ataaaaaggt atttgaatat gattctttaa tccctaatga gataatactg
     3121 caaaaagtag acatgctaac taaaaagctt gagtc
//
LOCUS       DAXLDT010000250         3153 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXLDT010000250 DAXLDT010000000
VERSION     DAXLDT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.251"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..2430)
                     /locus_tag="ACF2SP_002999"
     CDS             complement(<1..2430)
                     /locus_tag="ACF2SP_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1449 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360759.1"
                     /translation="MQEKMILDKSNIINAIFSNLSADEIRKVKGISHAFYDRATEYLT
                     GHLENENFHLNRVIPPSDAIQREQKIKFCAKFLQTLIRSISNGEEGSSGQLYKFNNMM
                     EKIRRAVQSNLGLSRVQRETYETFHPIYVKLGAPNFPINDEGELDPGSIVYRTDHVKG
                     RDERKSSWLVLLKDDSNEDGLSICFDTKFRKYLLHIDGEGHVTSQLTLRWNAIYDDLL
                     PDTAMQQPLRSFTPWQRHAEANFFNGRSTNKILGLLTKNYPEIFNYDPTSYFKEEPVF
                     SVKLNRNAPKKLSTDKKNIYLTLDESFFKTPNGFETGLNRQPILAVIKTNSPLRGDCF
                     SVVPAIYEKQKLHILIKGHQQQTQVLDVFHANFKTGGLLSWELLDDVLIFGEPSTEYK
                     PALTDLNLLSDQQSELMSHVSTAPMVRGSGPGARQYWLNGFFKSTVYSEMIVIAEIFN
                     RRFMSTPLASLEQTLNHSDATKSFYRLQFSRQELEKAKEFVFQLAIPNEFSKQEDFDF
                     FYGGDGNKLEEITKLAILMLKRMVPKEFHHKIRIGINMCCPAASVENIGSGFGLRNKP
                     DFIERIVKAIKDADSNLKVEFKTLMLSHEDGYGDGDQISGNTKNISEIQNERLTRIVY
                     KTKANRLVYQGKPRNIETYNVDTVSCIIREVNTPKEFQFGYSGMVATITNHDINQLGY
                     KVVGEPHYSVQSILEKIKKQLGEVPTEFEVMLGRVLLGSAWVLQGRYASDAEILLHTL
                     LQAAIEQEWARGMGRYGIDIYQIQVLYNIRHITNDNYRNSLINKILNAQSTEEIIDKI
                     FEQYFSIKKKGE"
     gene            complement(2816..>3153)
                     /locus_tag="ACF2SP_003000"
     CDS             complement(2816..>3153)
                     /locus_tag="ACF2SP_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD2360760.1"
                     /translation="NLDVLEQYSLYFKDQFNSIISQFEKNKIILPYNATCCQSNKINK
                     EIKRSLLSPRQKDCAKLLLQGMSYKEIGKILQLSARTVETHVNQLKTKLGCDNKAELI
                     LQLNGMITR"
BASE COUNT          963 a          599 c          489 g         1102 t
ORIGIN      
        1 ttcccctttc ttcttaatgc tgaagtattg ttcaaatatc ttatcaatta tttcttctgt
       61 agattgagca ttaagtattt tattaattag agaattccta taattatcat tagttatatg
      121 tcgaatatta tatagaactt gaatttgata tatatcaata ccatatcgcc ccatccccct
      181 ggcccattct tgctcaatcg ctgcctgaag cagcgtatgt aaaagaattt ctgcatcact
      241 ggcatatctt ccttgtaaaa cccatgctga gcctagcaaa actcgaccta gcatcacttc
      301 aaattcagtt ggaacttccc ctaattgttt tttaattttt tcaagaatac tttgtacact
      361 gtaatgtggc tcaccgacaa ccttataacc taattgattt atatcatgat ttgtaatagt
      421 agctaccatt ccgctatacc caaactggaa ttccttaggt gtattgacct ctctaataat
      481 acatgacaca gtatctacgt tataagtttc aatattgcgt ggctttcctt gatacaccaa
      541 tctattagcc ttcgttttat aaactattcg tgtgagacgt tcattctgaa tttccgagat
      601 attttttgta ttgccagata tttgatcacc atcaccatag ccgtcttcat gtgataacat
      661 taaagtttta aattcaactt tcaagtttga atcagcatcc tttatcgctt taacaatgcg
      721 ctctataaaa tcaggcttat tacgcaaacc aaagcctgaa ccaatgtttt ccactgatgc
      781 agcagggcag cacatattaa taccaatacg aattttatga tgaaactctt tgggcaccat
      841 tctttttaac atgagaatag caagctttgt tatttcttct aatttattgc cgtccccccc
      901 ataaaaaaaa tcaaaatctt cttgcttgga aaattcattg gggatagcca actgaaaaac
      961 aaactcttta gccttttcta attcctgacg actaaattgc aatcgataaa agctttttgt
     1021 tgcgtctgaa tgattcagtg tttgctccaa ggacgctaac ggtgtgctca taaaccgtct
     1081 attgaaaatt tcagctatta caatcatttc actataaact gtgcttttga aaaacccatt
     1141 taaccaatac tgtcgggcgc caggtccgct tcctcttacc attggtgccg ttgaaacatg
     1201 agacattaac tcggattgtt gatcggataa aagatttaaa tcagttaatg caggtttgta
     1261 ctctgtcgag ggttctccaa aaattaaaac atcgtcaagc aactcccatg acaggagtcc
     1321 tcctgtttta aaatttgcat gaaaaacatc aagtacttga gtttgctgtt gatgcccctt
     1381 aattagaata tgaagcttct gtttttcata aatggctggt accacagaaa aacaatctcc
     1441 tctgagtggg gagtttgttt ttatcacagc caatattggc tgtctgttta aaccagtctc
     1501 aaaaccattt ggcgttttaa aaaaagattc atctaaagtt aagtaaatat tctttttatc
     1561 tgttgacagc ttttttggag catttctatt taatttaacg ctaaaaacag gttcctcttt
     1621 aaaataagag gtaggatcat agttgaatat ttccgggtaa ttttttgtta ataaccccaa
     1681 tattttattg gttgaacgtc cattaaaaaa atttgcctca gcatgccgct gccagggagt
     1741 aaaagatcta agtggttgct gcatagcagt gtcaggcaat aaatcgtcat aaatagcatt
     1801 ccatctaagt gttaattgag aggtaacatg accttcacca tctatatgga gtagatattt
     1861 acgaaattta gtatcaaaac aaatggaaag tccgtcttca tttgagtcat ccttcaacaa
     1921 tactagccat gatgattttc tttcatctct ccctttaaca tgatcagttc gataaacgat
     1981 gctgcctgga tcaagttccc cctcgtcatt aattggaaaa ttgggcgctc cgagtttgac
     2041 ataaatagga tggaaagttt catatgtttc tcgttgaaca cgacttaaac ccaggttaga
     2101 ttgtacagca cggcgtattt tttccatcat attattaaat ttatacaatt gcccagatga
     2161 tccctcctct ccattagata tactacgaat aagtgtctgc aaaaatttgg cacaaaattt
     2221 aattttttgc tctctttgta tcgcatcaga aggtggaata actctgttaa ggtgaaaatt
     2281 ttcattctcg agatgacctg tcaaatactc agtggcccgg tcataaaaag catgcgatat
     2341 gcctttgact ttcctaattt catccgccga aagattacta aaaatagcat ttataatatt
     2401 tgacttgtct aatatcattt tttcttgcat ttacaacatt ctctttttga cgcgttatcg
     2461 caatttcaat tcaccttcaa ttttttctgc acccaatgat cagtcttgaa ataatatttt
     2521 caaaatttta taaattatgg gcatagcaaa gaaaaaaact cgggccttgc cgtattcgaa
     2581 tatcctctca agccatattt acacgtaaat atgattaagc ttttttgttc ggggactatt
     2641 tctactcatt gataaagcct tacgtagaga cgagaagcaa tgctgctgtc caattgtaaa
     2701 aggaattggg aatttaactt tattctgcat ttaagtactc tcttgtataa aaacataaag
     2761 aatacttaaa gtaacattca gagaatatcc gtaaaatcac ggataaaata gctatttatc
     2821 ttgtaatcat tccgttcaat tgaagaatca actcagcttt attatcacat cccaattttg
     2881 tttttaattg attaacgtgg gtttcaacag ttctagctga taattggagt attttcccaa
     2941 tttctttata actcattcct tgcaataaga gttttgcaca gtccttttga cgaggagaaa
     3001 gaagggatct tttaatttct ttattgattt tgttgctctg acaacaagta gcattatagg
     3061 ggagtattat tttatttttt tcgaattgac ttattattga attaaattga tctttaaagt
     3121 aaagactata ttgttctaga acatccagat tat
//
LOCUS       DAXLDT010000251         3131 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXLDT010000251 DAXLDT010000000
VERSION     DAXLDT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.252"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            33..2030
                     /locus_tag="ACF2SP_003001"
     CDS             33..2030
                     /locus_tag="ACF2SP_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFD2360761.1"
                     /translation="MKKKHSIFSYFSQFLLFNLCFFGLQLLLLYSHAESLISSIKLPL
                     PIYFEIIGTLVIQFSLYIIISMIQALLLIGILNRSWHYFSTEQWQTIVWSLFACAILS
                     ANAYYFPLSVFSKLFSPPIPEFIFLILLYFSLACLGLLLLNCLFYRMTARLLSIVLPI
                     SICIFLLNNYWQSPLNPNVDSEPNVIILGIDSLSPESVTSRNMPFLHQVLLDSFQFTN
                     TISPLARTYPAWSSILTGLYPKHHLAEENLVERSRVKSQLSIVWDLNELGYNTIYATD
                     DRRFNSIDENFGFKKIIGPKTGVNDVMLGSYNDFPLGNLLINFRISAWLFPYNFSNRA
                     SFISYYPETFTNELKRELSLQQNSPVFLAVHFALPHWPYAWAASLPDQVENEFSLSKR
                     DALYLRALKTVDNQFKVIFCYLKKHGYFKNSLVIILSDHGEVLYYPNSRLTSYQKYQS
                     SLTSKLAEYFKAKTATELDKSAGHGSDILSPMQYHSLLAFNVYKDGKLLTKTNKIKTR
                     VALFDLAPTILDFLNITKKQKMDGISLLPTVLNPNTPLPHRTFFIESGMYPNQDFTKE
                     KAIELGKKIYKVNPDTGQLELKPDELIQVNNQKLYGVISGNWILALYPDDNTYIPVIL
                     NLSTGEWIDNLQSDFAKSSPAEKLKKQLQEFYGKKLVYPIQ"
     gene            complement(2278..>3131)
                     /locus_tag="ACF2SP_003002"
     CDS             complement(2278..>3131)
                     /locus_tag="ACF2SP_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360762.1"
                     /translation="IFFYKLYPDKLFNELIHPEKLVSVRMRLGFLHEFIELLQKQLHS
                     NALQYGLNPGIDFLFHGEELPQGIMIEVNEVYRDIIQLAVKNLRVQFTPENNEKVTLE
                     RLHDLGRAYKFWFNPNRLIDAVMVLQQRLVTESISQEEDLEKFHQEMLVLYRQLTTTE
                     CLDLYGYFANNDSRYLLYTLYSIINGYSLDWLPSLNASERNAIARVYQALKCVMEALR
                     VELKNRFITTEPYLYDLGKKEIHTGRRNREAVFRIIAIYGCERIVMSDSLERLFNLIE
                     EPNQQFE"
BASE COUNT          996 a          617 c          504 g         1014 t
ORIGIN      
        1 tggcatatta attgtcctgc agacaatcaa tcatgaaaaa aaagcattcg attttttcat
       61 attttagcca attcctttta tttaatcttt gcttttttgg tttgcaattg cttctgctct
      121 attcgcatgc tgagagccta atcagttcga ttaagcttcc tttgccaatc tattttgaaa
      181 ttatcggcac tcttgtaatt caattcagcc tgtatatcat catttccatg attcaagccc
      241 tgctgcttat aggcatttta aatcgatctt ggcattattt ttctactgag caatggcaaa
      301 ccattgtttg gtccttattt gcatgcgcta tattaagtgc taatgcctat tatttccctc
      361 tgagtgtttt tagcaaacta ttttcaccac caattcctga atttattttt ttaatattac
      421 tttatttttc tttggcttgc ctggggctac tcttattaaa ttgcttgttt tatcgcatga
      481 ctgctcgcct attatcaata gtgctaccca ttagcatttg tattttccta ttaaataatt
      541 attggcaatc tcccttaaat cctaatgttg attccgaacc caacgtcatt attctgggca
      601 tagacagctt aagtccagaa agtgtcacat ctagaaacat gccttttctt catcaagtgc
      661 tactggacag ttttcaattt acaaacacga ttagtccttt agccagaacc tatcccgctt
      721 ggagctccat tttaactggg ttatacccta aacatcatct tgccgaggaa aatttagttg
      781 agcgaagccg ggttaaaagt caactcagca ttgtgtggga tttgaatgaa ttggggtata
      841 acacaatcta tgcaactgat gatagacgat tcaatagcat agatgagaat tttggattta
      901 aaaaaatcat cggcccaaaa actggtgtta atgatgtcat gttaggctct tataatgatt
      961 tccctcttgg gaatttactg attaattttc gcattagtgc ctggttattc ccctataatt
     1021 ttagtaatag agccagcttc atatcctact atccggaaac ctttacaaat gaattaaaaa
     1081 gagaactgtc tttacaacaa aattcccctg ttttccttgc ggtgcatttc gctcttccac
     1141 attggcctta tgcctgggca gcatcattgc ctgatcaagt ggagaatgaa ttcagcctca
     1201 gcaagcgtga tgcgctttac ctaagagcgc taaaaacagt agacaatcaa tttaaagtca
     1261 ttttctgtta tttaaaaaaa catggctatt ttaaaaatag cctggtcatt atactaagcg
     1321 accatggcga agtactttat tatcccaact cacgattgac aagttaccaa aaatatcaaa
     1381 gctcactaac tagcaaactc gcagagtatt ttaaggccaa aactgcaact gaactggata
     1441 aaagtgctgg ccatggttct gatatattaa gccctatgca atatcatagc cttctggcat
     1501 ttaatgttta taaagatgga aagctactta caaaaacaaa caaaatcaaa acaagagtag
     1561 cattatttga tttggcccca accattctgg acttcctgaa tattacaaaa aaacagaaaa
     1621 tggatggcat ttcattacta cctactgtat taaacccaaa tactccacta ccacaccgca
     1681 cattctttat tgaaagtgga atgtatccaa atcaagattt tactaaagaa aaagccattg
     1741 aattaggaaa aaaaatctat aaggttaatc cggatactgg gcaacttgag ttgaagcctg
     1801 atgaattaat tcaggtgaat aatcaaaagc tatacggagt cattagtgga aactggattt
     1861 tagccttata tccagatgac aacacctata tacccgttat tctcaatctt tcaactggag
     1921 aatggattga taacctgcag agtgattttg ccaaatctag tccagcagaa aaactaaaga
     1981 aacaacttca agaattctat ggtaaaaaac tggtttatcc cattcaataa aagcgcttta
     2041 taaagctcac tgcaaagccc ccatacaaaa cagcagacct attgcagaac ttgattgttt
     2101 tgtttcagag caaggcgcaa acgttttgag aagcagagtt tatatgtagt aaatgagcgc
     2161 cgcagaaacg tttgcaatgc agcagtaaaa tgaaagaagc aagttataaa agaggttggg
     2221 tataccccaa aacacgaatt actcttagtc aattggcacc tgacaaattc atatttacta
     2281 ctcaaattgc tgatttggtt cttcaatgag attaaacaat ctttctaatg agtcactcat
     2341 cactattctt tcacaaccat aaattgcaat gattctaaat acagcctctc gattacgtct
     2401 tcctgtatga atttcctttt tccccaaatc atatagataa ggttcggttg tgataaatct
     2461 gttttttaat tcgactctta gcgcctccat aacgcacttc aacgcttgat acactctggc
     2521 tatagcattt ctttcagatg catttaatga tggcaaccaa tctaaagagt aaccattgat
     2581 tattgaatat aaagtataaa gtaaatatcg actgtcattg ttggcaaaat agccgtataa
     2641 gtccagacat tctgttgtgg tgagctgacg atatagaact agcatttctt gatgaaattt
     2701 ttctaaatcc tcctcttgag atatgctttc cgttaccaga cgttgctgca gaaccatcac
     2761 agcgtcaatt agcctgttag gattaaacca aaatttgtat gcccttccca aatcatgtaa
     2821 acgttccagg gtaactttct cattattttc aggtgtaaat tgcactctaa ggtttttaac
     2881 agctaattgg attatatctc tataaacctc attcacttcg atcataatgc cttggggtag
     2941 ttcttcacca tgaaatagga aatcaatacc tggatttagc ccatattgaa gcgcattgct
     3001 atgtaattgt ttttgtagaa gttctataaa ttcatgcaaa aaaccaaggc gcatacgcac
     3061 tgaaaccaat ttttcaggat gaattaactc attaaataac ttatcaggat acaatttata
     3121 aaaaaatata g
//
LOCUS       DAXLDT010000252         3104 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXLDT010000252 DAXLDT010000000
VERSION     DAXLDT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.253"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            153..1412
                     /locus_tag="ACF2SP_003003"
     CDS             153..1412
                     /locus_tag="ACF2SP_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360763.1"
                     /translation="MNKRLFIVFILGFSSGLPMALISSTLQAWYANNGMSVLATGALS
                     LVSLPYAYRIFWGPILDRYSLFHLGKRRSWILTMQILLLLGFNLMAWFIPAQYPKLMA
                     FLALVLACFSATQDVAIDAHRAEYLPPQEHALGASLAVFGYRLALLLSGGLALVMAEK
                     LGWAFTYRFMGFAMAIGMLAIFVSKEPSTEIKEKSNFLVSFIAPVKELLSRPGIIYLL
                     IFIFCYKLGEAFTTTTSGIVMPFLIQGLGFSLDTIGYINKMLGIVSILLGGLTAGFLL
                     MRYSLYRALFFFGLLQALTNVFFVILAMSGKNIVLLAIAVFSDNFVAGMGSTALVALF
                     MRLVNKQFTGTQFSMLVALSTLPRIISGPVAATIQMHIGWVGLYQLSIVFALFFIPFL
                     VLIKEQTKNMSQQELEIEKSGDLNPIR"
     gene            1524..2600
                     /locus_tag="ACF2SP_003004"
                     /pseudo
     CDS             1524..2600
                     /locus_tag="ACF2SP_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611491.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          957 a          524 c          630 g          993 t
ORIGIN      
        1 aaagtcatac aaaacgcatc cttctcattt agagagggat gctctctttg taaaggctaa
       61 tgatcttcat aaaaggtgtc agcctaacac cactgaggaa attaaaatat gtctgtttta
      121 aaggctatga ctcatactga gtgaacaaag gaatgaacaa gcgcctgttt atcgttttca
      181 ttttaggttt ttcttccggt ttacctatgg cattaatcag cagtaccttg caagcatggt
      241 acgctaataa tggcatgtca gtgcttgcga cgggagcttt aagtttagtc agtctacctt
      301 atgcgtatcg tattttctgg ggtccaattc ttgatcgcta ctctttattt catttaggga
      361 aaaggcgaag ctggattctc accatgcaga tactactgct tctgggtttt aatctcatgg
      421 cctggtttat tccagctcaa tatcctaaat tgatggcatt tttagcttta gtattagcct
      481 gtttttcagc cacacaagat gtggcaattg atgctcatcg agcagaatat cttcccccac
      541 aggaacatgc attgggcgcg tctctggctg tgtttgggta tcgattagct cttttgttat
      601 ctggcggttt ggctttggta atggcagaaa aattaggctg ggcttttact tatcgcttta
      661 tgggatttgc aatggcgata ggtatgttgg caatatttgt cagcaaggaa ccctctacag
      721 aaataaaaga aaaaagtaat tttcttgttt cgttcattgc accagtgaaa gaacttttaa
      781 gccgcccagg gattatttat cttttaatat ttatattttg ttataaatta ggtgaagcat
      841 tcactacgac aactagcggt atagtgatgc cttttttgat ccaaggcttg ggtttctctc
      901 tggatactat cggctatatc aataaaatgc ttggaatagt ctctatcctg ttaggcggtt
      961 taacggcagg ttttttatta atgcgatatt ccttgtatcg cgcattattt ttctttggct
     1021 tactacaagc actaaccaat gtcttttttg tcatattagc gatgtcagga aaaaatattg
     1081 tgctgttagc aatcgcagta ttttcggata atttcgtcgc agggatgggt tctactgcat
     1141 tggttgcttt atttatgcgt ttggtgaata aacagtttac tggaacacaa ttttctatgt
     1201 tagtcgcatt atcgacttta cccaggatca tttcaggtcc tgtagctgct accattcaaa
     1261 tgcatatcgg ctgggtcggt ttatatcagt tatccatcgt tttcgctttg ttttttatac
     1321 cttttctagt gttaatcaag gagcaaacaa aaaatatgtc acagcaggag ttggaaattg
     1381 aaaaatcggg agatttaaat ccgataagat gatttattat tttatagaac aaaattttta
     1441 tagatgtaat tgtaaccccc tctaaaatag gtgcatttct gattagagtt ctccaagtta
     1501 aactatgttt gaggagaaca aaaatgaaaa aatcacgtta tacaaaaaca caaattgtaa
     1561 aaattctaaa agaagtcgaa gcaggtagtt tggtcaaaga aatttgtagt gaatatggaa
     1621 tttcagatgc aacctattac aactggaaag caaaatatgt tggtatggag gcatccgacg
     1681 taaaacgtct aaaagatctt gaagaagaga accgtcgtct aaagcaaatg tatgctgagt
     1741 taagtcttga tcacaaaatc ctaaagaata ttgtcgaaaa aaagctgtga agccatctgt
     1801 gagacaggag gtggttgatt atgcagtgaa tactcatact gtgagtttac gccgtgcttg
     1861 taaggttgag ggtatcagcg actcggttta tcgatataag ccggatagcc aagctgatga
     1921 aggcgttatt gcagcattgc aggaatcatc cgaacgttat ccggcgtatg gctttagtaa
     1981 attgcttaaa gtcttacgcc gtcagggtca taagtggaat cataaacgca tttatcgggt
     2041 atattgtgaa ctgaagctca atatgcgtcg taaaggcaaa aaacggcttc ctaatcgctc
     2101 acctgccccc cttgagtgta ccagcatcca ttaatcagtg ttggcctatg gattttatgt
     2161 gtgatgcgtt gatgtgtggc cgacgtttca gaacatttaa catagtagat gattttaacc
     2221 gtgaagtgtt ggctatcgaa atcgatttaa gcctgcctgc gcaaggagta attcgcgtat
     2281 tagaacgcgt tgttgcatgg cgaggtttcc ctgctaaatt acgcatagat aatggtcctg
     2341 aatttatctc aagcacatta gcagactggg ctgaaaagca tcaagtggtt ttagagttta
     2401 ttaagccagg taaaccaacg caaaattcat ttattgagcg attcaataga acttaccgca
     2461 cagagatatt ggatatgtac gcgtttaaaa atctacaaga agtcagagag ctgacagaaa
     2521 tctggataaa ggagtacaac gacgagagac cgcacgattc gctaaatgat ttaacaccgt
     2581 gggaatatct ggcaaaaaat aaggcaatga actctaattt aggttgccac tatatatggg
     2641 tggtacaagt tacaaacctt gcgtttttaa atatgaattt acctcattcc ccttcctgtc
     2701 ttcattttgt agcaacaatt aatttgaggc aattcgggga aacctatcaa aattgagtat
     2761 ttaatgttca cttaatgtaa atttgctaaa atgtttgtca aaaatatacc atttcgagag
     2821 aaaatgaagc tgatgaactt aatacatata ttttaaaaat acgaaagtat ttcaaaagtt
     2881 gaaatatttg atttagttaa aaatctcatg cttctaaaat ctcgagtttc tagtctttat
     2941 ggtaaaccga aagactctat atcgggtgaa actaagaaat gctttaaata aaagtgacac
     3001 tatttaaaaa taaaaagaat ttcaatacac atattgaata gtattacaaa aaactgaatt
     3061 tgatgttctt gagacgatgc atcatcaata aatagcaaat tcaa
//
LOCUS       DAXLDT010000253         3082 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXLDT010000253 DAXLDT010000000
VERSION     DAXLDT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.254"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..734
                     /locus_tag="ACF2SP_003005"
     CDS             <1..734
                     /locus_tag="ACF2SP_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360764.1"
                     /translation="NSDHFEVIDTELDNIFQAITLDMAYNSNNFRFIDILGYFINKFI
                     KEHNSQSATVFAYGKAEQEINPQGTKYYLKTLAQYCNNTTEFLKNAQTNTTLTATNLL
                     DCYRYNISLQVYRTQIRIICSIKEINKLKVTNETSEQITLLLKSFQQLAEFAKIHCFL
                     LKTDSSCYRLFKLIENKAMQSTLSRDWIRQLDAKEALVYMMDDLPNQLKLTADYNASS
                     LFFSSAKQAKREKTENSEEPIMKLG"
     gene            complement(999..2215)
                     /locus_tag="ACF2SP_003006"
                     /pseudo
     CDS             complement(999..2215)
                     /locus_tag="ACF2SP_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
     gene            complement(2536..3045)
                     /locus_tag="ACF2SP_003007"
     CDS             complement(2536..3045)
                     /locus_tag="ACF2SP_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360765.1"
                     /translation="MKKWNSFLLLIYLSLFIGHAHAITSLLTNTSLLTNFAQLVKAVE
                     QGDDVKAIIRLDRCQITDPALQTQLVQNLDGASTRFNFTEYLHFRTRINDQLRDTVTT
                     VSSKIFEYSPGVFLSVSSRLNVFEDNTATLRVNFYNPALGTSVLIIDWLCDISNGNDE
                     NGLFLYNSP"
BASE COUNT         1113 a          595 c          488 g          886 t
ORIGIN      
        1 aaaacagcga tcattttgag gtaattgata cggaattgga taacattttt caagccatca
       61 ctcttgatat ggcttataac tccaataatt tcagattcat tgatattctt ggctacttca
      121 ttaataaatt tattaaagaa cacaactccc agtccgcaac agtatttgct tatggaaaag
      181 cagaacagga aatcaaccct caaggcacaa aatactatct caaaactctt gctcaatact
      241 gtaacaatac gaccgaattt ttaaaaaacg ctcaaacaaa tacaacatta acggccacaa
      301 atctccttga ttgctatcga tacaacatca gtttgcaagt ttatagaacg caaattcgga
      361 ttatctgtag cataaaggaa atcaataaat taaaagttac gaatgagact agtgaacaaa
      421 taacccttct actcaaatcc tttcaacaac ttgcagagtt cgcaaaaatc cattgtttct
      481 tgttaaagac tgactcttct tgttatcgat tatttaaact gattgaaaac aaagcaatgc
      541 aatctacttt atccagagat tggataagac agcttgacgc aaaagaagcg ctcgtttaca
      601 tgatggatga tctgcctaat caattaaaac ttacggcaga ttacaacgct tcgagtttat
      661 tttttagttc ggcaaaacaa gctaaaagag aaaaaacgga aaactcagaa gaacctataa
      721 tgaagctagg ctaaattaat ttaaagggat tattggtaaa tataaacaca attgattggg
      781 gtctggatgc ccaacacaag gacatccagg tttttctata agcttgattt ttttgaggag
      841 ttacattgac ccctttcgca atagaacggg acttaccaaa aactccaagg tcgaggcaaa
      901 aaatgttttt aatgaggtat tttagataaa ctaaatgacc gaattaaaaa cattttttaa
      961 cgatgagatt ggggtttttc gtgagtcctg tagaaatctt acatctcact ggcataaaca
     1021 cttttggttt ctgcttgtcc tttttcttgc aattcaaatt gagttttctg gtaatccaaa
     1081 gattgttgcc acctggaaat aataatcgtg gcgattgtat tagccatcat attaataatt
     1141 gacctggctt cattcatgaa tttatcgata cccaaaacaa gaacgacgcc tgcagcagga
     1201 atgtgtccta tcgttgctaa tgaactcgcc aacacaataa atccacttcc ggacacccca
     1261 gcggcaccct tggaactaat gatcataact cccagaagcg ataactcctg ccaaactgtt
     1321 agatcagtat tagtagcctg tgcaataaac atggcagcca ttgttaaata gattgcagtt
     1381 tctgcatcaa acttggcagt acggtttcgg aggaagcagt gctccacacg attaaaagct
     1441 catcctttaa ataccttaac aaatgccaaa tattaatttt gcagtaccat tgcaaaagac
     1501 ttcccaagat aatcaagata aagaaaatgg aagttaggta ataacaagcc aataatccaa
     1561 ttagattaaa caaagggcca actccaaatt tcccgacact gaatgccatg gcagcaaagg
     1621 cagctacagg cgccatatac attacaaaat gaataatttt aaaaaagaca ttagacagga
     1681 cctgcattcc agtaaccaat gatttacctg cattgcctgt aagaatcagg ccgatcgcaa
     1741 acaatattga aataaaaata acttgcagca gttcaccatc gacaaaagcg ctaataaaag
     1801 ttctcgggat aatattaatt agcaaatcag tcacattggt taccttatgc gccccctcaa
     1861 ggtaagaact cgcttccttg acatccagag aattgggatt cacatttaaa ccaacaccag
     1921 gcttaaaata atctgtcgcc agaagaccta tcagcaaagc cagggttgtg atgaacgtaa
     1981 aaaaaagcaa tgccccaccc gccaagcgtc caacttgcct catatcattc attgctgcaa
     2041 ttccagagac cagagtgaga aatataatag gggaaattaa catcttaatg attttgatga
     2101 aaacatcggc tatggctttt aattgaaccc ctacatcagg cataaaatat ccaagtaaaa
     2161 taccaatgaa aacacccaga agaacttgta catataattt tttaagaaaa ttcattttta
     2221 ttctccaggt cgtttactat aaacaactat tcaaaagcaa agcatgaagc actaaaaatt
     2281 ctttccctag acaaatcatt attacatgaa tagtttaaat tattctagat atgatcaata
     2341 ttgatatatt ttttcaattt taactattat tacaacaatt tgaagagatt ttgaaaaata
     2401 aaattgcaga attggttatt taatttgctt gatactcgct tattatttta gataaaccct
     2461 aataagatta ggcaaatatg agcaatcatt aagaagttaa ctaaatgaaa agagtaaact
     2521 ttatacctcc agagcttaag gactgttgta taaaaacaat ccattttcat cattgccatt
     2581 actgatatca caaagccaat cgataattaa cacacttgtg cctaaagccg gattatagaa
     2641 attcacacgc aaggtggcag tattatcttc aaagacattc aaacgacttg aaacagataa
     2701 aaatacaccc ggcgaatatt caaatatctt gcttgagaca gtagttaccg tatctcgtaa
     2761 ttgatcatta attctggttc gaaaatgcaa atactctgta aaattgaatc ttgttgaagc
     2821 gccatccaaa ttctgtacta attgagtttg cagtgcggga tctgtaattt ggcatcgatc
     2881 caatcgtata atggccttga catcatcacc ttgttcaact gctttaacta attgcgcaaa
     2941 attagtcaac aaggaagtgt tagttaacaa agaagtaatt gcatgggcat ggcctataaa
     3001 taaagacaaa taaattaaca aaagaaagct attccatttc ttcataacgt cccctcttat
     3061 tgattcatta tttgcttaat ct
//
LOCUS       DAXLDT010000254         3069 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXLDT010000254 DAXLDT010000000
VERSION     DAXLDT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.255"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     repeat_region   150..3056
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=225..261
                     /rpt_unit_seq="gtttcagtggttggatttttagatgagggattattgg"
BASE COUNT          830 a          285 c          828 g         1126 t
ORIGIN      
        1 ttacaaataa ttggtaaaaa tgaccgatta tattttctga tgtttgtcga agaaattaaa
       61 atacaacaaa attaaattct gtttaattat ataaaataat accccatgga tttttactat
      121 aatagtatac agagaataaa gtatggagta tttcattagt taaattttta gatgagggat
      181 tattgggctg taaccataaa tggtgctgtt tctggcgcaa ttaagtttca gtggttggat
      241 ttttagatga gggattattg gcggacggtg agcatttcac accaattccc aattatggtt
      301 tcagtggttg gatttttaga tgagggatta ttgggagcca tatgaggaaa ttgtgacaag
      361 gtataggagt ttcagtggtt ggatttttag atgagggatt attggcttct atggcgatgg
      421 cgttacccca atcaattcct agtttcagtg gttggatttt tagatgaggg attattggtt
      481 caaatggcgg cacgtgagtc acaagaatta tttgtttcag tggttggatt tttagatgag
      541 ggattattgg aactacttta gcaagtaccg ctaaggagta tcgaagtttc agtggttgga
      601 tttttagatg agggattatt ggacaaaatg ctctaaacaa ctacctatct ggaatagcgt
      661 ttcagtggtt ggatttttag atgagggatt attggtcgaa acataggaaa tgatgatatt
      721 gtctaagtat agtttcagtg gttggatttt tagatgaggg attattggga tgaagcagaa
      781 ggtaaggcgc ataaattaag attgtttcag tggttggatt tttagatgag ggattattgg
      841 taaaatgaat tactctattg ttttaaaata tgagaagttt cagtggttgg atttttagat
      901 gagggattat tggttagaac taatagccca aagaaacgta tacttaagtt tcagtggttg
      961 gatttttaga tgagggatta ttggaatatg atgtgtaacg ttcccattct gagaagtttc
     1021 agtggttgga tttttagatg agggattatt ggacaatcat atgggttttg atgactgctg
     1081 atttaggttt cagtggttgg atttttagat gagggattat tggtttgatg acgtcttgca
     1141 aacaatcgac caatatctta gtttcagtgg ttggattttt agatgaggga ttattggtta
     1201 ataaggaagc aataatgatt aaattaaaac gtttcagtgg ttggattttt agatgaggga
     1261 ttattggtag tagatgtcta gttaatcaag cttacgataa aaagtttcag tggttggatt
     1321 tttagatgag ggattattgg cacaaggaaa aggaaatgaa atctgaaaaa agaagtttca
     1381 gtggttggat ttttagatga gggattattg ggttacttaa ccaataaacc gccatcccga
     1441 tgttataagt ttcagtggtt ggatttttag atgagggatt attggcccgc gcacgcgcgc
     1501 gaaactttgg gttattgctg tttcagtggt tggattttta gatgagggat tattggtatg
     1561 aaaaactgag tgaaatgaac gtaacaatgt agtttcagtg gttggatttt tagatgaggg
     1621 attattggtc aacacggtct cggtatgttg tttcgacttg gtgtttcagt ggttggattt
     1681 ttagatgagg gattattggt tcaagtctgg cgcgggttgt ggcgggtttt cagggtttca
     1741 gtggttggat ttttagatga gggattattg gcttttagtg ttgtgtctaa aaaacaaacg
     1801 tttgtcagtt tcagtggttg gatttttaga tgagggatta ttggttctta taataacacg
     1861 atcaaactag cttgcaatag gtttcagtgg ttggattttt agatgaggga ttattggttt
     1921 gataaaagcg gtctatgcgg ccgacaaatt cgtttcagtg gttggatttt tagatgaggg
     1981 attattggca ttatgttgag gcataaggac gtttcagtta tggagtttca gtggttggat
     2041 ttttagatga gggattattg gacgcctatc aatatgtgtt aaaaggtaaa atgtttcagt
     2101 ggttggattt ttagatgagg gattattgga ttacaaggcg tttttaataa atcctttgcg
     2161 tcagtttcag tggttggatt tttagatgag ggattattgg tattatgtgc caatcttcta
     2221 aaatattatt aatgtttcag tggttggatt tttagatgag ggattattgg gattcacaat
     2281 atccgccctg agggtaagat gtgaggtttc agtggttgga tttttagatg agggattatt
     2341 ggttcgaagc ggaggatcgg cgttggccta aagtgagttt cagtggttgg atttttagat
     2401 gagggattat tgggccaata gcattatttg ttgagcaatc aggttaggtt tcagtggttg
     2461 gatttttaga tgagggatta ttggagatta cctaagtcat atcagaacgt ttatacaaag
     2521 tttcagtggt tggattttta gatgagggat tattggtcta ataattcgtg gcatctggtt
     2581 tctacattca gtttcagtgg ttggattttt agatgaggga ttattgggta acatttaata
     2641 atttgcactt atgtattcta tgtttcagtg gttggatttt tagatgaggg attattggtt
     2701 gaaaaagaga ttgagttacg attaactggt aggtttcagt ggttggattt ttagatgagg
     2761 gattattggt aaaccttcta gtacaaaagg ctcgcttgtt attggtttca gtggttggat
     2821 ttttagatga gggattattg gatagatgtt acagtgtcta gtgaaccagt gcctggtttc
     2881 agtggttgga tttttagatg agggattatt ggcttaattc acacagagac actaggattg
     2941 tattcgaagt ttcagtggtt ggatttttag atgagggatt attggttata gcaaaaatta
     3001 taaccttcag gagtagtgag tttcagtggt tggattttta gatgagggat tattggatgt
     3061 gcgctagaa
//
LOCUS       DAXLDT010000255         3066 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXLDT010000255 DAXLDT010000000
VERSION     DAXLDT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.256"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(358..1269)
                     /locus_tag="ACF2SP_003008"
     CDS             complement(358..1269)
                     /locus_tag="ACF2SP_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFD2360766.1"
                     /translation="MTIQKGLHERRALKISIAVTFLLAVVGILFGLLSGSLAIVFDGM
                     FNMVDTVISILAFFVARLLTSKGNRRFQYGYWHIEPMVLVLNGSILILLCTYALVNAI
                     GSLMSGGHELNFDWAFVFALLVFFLSTGMYFYLVKTNRKIKSEFLRLDIQSWLMSALI
                     STSLLLAFGIAALLHGGAYGYFTPYIDPLILAILTAFLIFVPMAAVRDAMRDIFRMAP
                     VDLDERIREFLDELIKQHDFKTYTSYVAKIGRAQFIEIHIVVPMDYPISSIETLDKIR
                     NEIALAIGENTPQRWLTIAFTANENWI"
     gene            complement(1426..2832)
                     /gene="dbpA"
                     /locus_tag="ACF2SP_003009"
     CDS             complement(1426..2832)
                     /gene="dbpA"
                     /locus_tag="ACF2SP_003009"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFD2360767.1"
                     /translation="MIQSEHNDQHLSFSQFPLRQELIKSLASSNYENMTPIQMQSLPI
                     ILRNEDIIAQAKTGSGKTAAFALCLLNNLKISFFAVQALVLCPTRELAEQVSQAIRRL
                     ACLMPNVKIINLSGGIPMQPQLDSLRHGAHIIVGTPGRILKHLKNASLDLSQVKALVL
                     DEADRMLDMGFFDDIKNIISVCPKQRQTLLFSATYPEEIKRLSKQFMKDPKEVHVVTP
                     PEEIDIEQHFYEVSRHAQKFPLLKSLLLHYRPVSVLIFCNTKQQTVEVTDQLINEGFS
                     AIALNGDMEQVERDLAVLRFANQSCSILVATDVAARGLDIKELSAVINFDLAFDHDVH
                     IHRIGRTGRAGSKGIALSIVTPADAQRICAIEDNYPHPIHWGNINALENHNTACLVPE
                     MVTLCIASGKKDKIRPGDILGALTKDAGLAGNTIGKINITAMYSYVAIHHSQADKAYQ
                     YLQSGKLKGRKVNVRKIN"
BASE COUNT          974 a          571 c          570 g          951 t
ORIGIN      
        1 gggtagatat tgttcctaat aaaacttata taataaacca agaccaactt gtgcctgcga
       61 ttctaatgtc tgggacaggc tgctaaaacc acctgtctct atgcagtgaa aagctatcaa
      121 tacctcatgt acctaagccc gttagtgata atgggctcta acccaaaata gcgatataac
      181 gtggtcaaag acccgctaag tgcttccatc acaaaatgaa gttttgttgc tggcaaacaa
      241 gttgttctat ttaatctgat tacatttact aaaattaaga gttcattttg ctgtactctc
      301 aattttcagt agcatgtttt cacaaatatc ctgatggaaa tttgaataaa acgcttgtta
      361 aatccagttt tcatttgcag taaaggcaat ggttagccaa cgttgtggcg tattttctcc
      421 aatagccaac gcgatttcat tacgaatttt atccagggtt tcaatgctcg aaattgggta
      481 atccataggt actactatat gtatttcaat aaattgtgcg cgtcctattt ttgcaacgta
      541 gcttgtataa gtcttgaaat catgctgttt aataagctca tctaaaaatt ctcttattct
      601 ttcatcaaga tccacaggag ccatgcgaaa tatgtctcgc atggcatcac ggactgcggc
      661 cataggcaca aaaatcagaa atgcagtaag aattgctaaa ataagggggt caatgtaagg
      721 ggtaaaatat ccataggccc caccatgcaa caaggctgcg atgccaaatg ccaacaggag
      781 agaagtagaa atcaaagcag acattaacca actttgaatg tctagtcgca agaattcgga
      841 cttaattttg cggtttgttt taactaaata aaaatacatg cctgttgata aaaaaaacac
      901 caaaagtgca aacacaaatg cccaatcaaa attgagttca tgtccaccag acatcaaact
      961 gccaatcgcg tttaccagtg cataagtaca aagaagtatt aggatactgc cattcaaaac
     1021 aagaaccata ggttcaatat gccagtaacc atattgaaat ctccgattcc ccttacttgt
     1081 taaaagacgg gcaacaaaaa aagcaaggat tgatataacg gtatccacca tattaaacat
     1141 accgtcaaag acgatagcca atgaaccgga taatagccca aataaaatac ctaccacggc
     1201 taagagaaag gtaacagcga ttgatatttt aagagctctt cgttcatgaa gtcccttttg
     1261 tattgtcatt tgagtgatat cctgatttta acaatgctct atctacttat actattgctt
     1321 caacagccac taataaatcc ataggtttgt attattaggc cttttacata atacctgctt
     1381 cttttttcaa ttgttgcagt aagtttttcg catggtttag aaaaatcaat ttattttacg
     1441 tacattaact ttgcgtcctt ttaattttcc actttgtaaa tattggtagg ctttatcagc
     1501 ttggctgtgg tgaattgcaa cataggagta catagcagtg atgttaattt tgccaattgt
     1561 gttgcctgct aatcctgcat ctttggttaa tgccccaagg atgtcaccag ggcgaatttt
     1621 atctttttta ccggaagcga tacagagtgt aaccatttct ggcactaagc aagcagtgtt
     1681 gtgattttct aatgcattaa tgtttcccca atgaatgggg tgcgggtagt tatcttcaat
     1741 agcgcaaatt cgttgtgcat ctgcaggagt tacaatgctt aaggcaatgc ctttactccc
     1801 agctcgtcct gttcgaccaa tacgatgaat atggacgtca tgatcaaaag cgagatcaaa
     1861 attaatcacc gcagaaagtt cctttatatc tagtcctcgt gctgcaacat cagtggcgac
     1921 aagaatagaa caactttggt tggcaaaacg caagacagca agatcacgtt cgacttgttc
     1981 catatcacca tttaaagcaa tagcactgaa gccttcattg atgagctggt ctgtgacttc
     2041 tactgtttgt tgcttggtat tacagaagat taacactgaa acgggccgat agtgtaacag
     2101 taatgatttt aataagggaa atttctgagc atgtctcgag acttcataaa aatgttgttc
     2161 aatatcaatt tcttcaggag gagttaccac atgaacttct ttcgggtcct tcataaattg
     2221 ttttgaaagc cgtttgattt cttcaggata agtcgcagaa aaaagcaggg tttgtcgttg
     2281 tttgggacaa accgaaataa tgtttttaat gtcatcaaaa aaacccatat ccagcattct
     2341 gtctgcctca tccaaaacta aagcttttac ctgagataag tctaaagagg cgttttttaa
     2401 atgcttaagt attctccccg gtgttcctac aataatatga gctccatgcc gtaatgaatc
     2461 aagctgaggc tgcattggta taccaccaga taaatttata attttgacat taggcatcaa
     2521 acaggctaat cgacggatag cttggcttac ttgttccgcg agttcacggg tggggcaaag
     2581 aaccaaggct tgtactgcaa agaaagaaat ttttaaatta tttaataaac acaaagcaaa
     2641 agcagcggtc tttccacttc ctgtttttgc ttgagctatt atatcttcat ttctaagaat
     2701 tatgggcagg ctttgcattt ggataggggt catattttca taatttgaag aagcaagact
     2761 ttttatcaat tcttgacgaa gggggaattg agagaatgat aggtgttggt cattgtgttc
     2821 tgattgaatc atggaaagtg cttattgtta taataggtga atagcttagc acttcttagt
     2881 gcaattttaa atcaatttat tttgggcaag cttaatctga tgcttacaat tgtcttttct
     2941 acttgaatcc gtgtagatca aaataacatt aaaatcatct caaagacatc aacagagagt
     3001 gattttaatg tttctatcag atgtatcatt ctacaaagag atataagtaa aaataatatt
     3061 agctac
//
LOCUS       DAXLDT010000256         3051 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXLDT010000256 DAXLDT010000000
VERSION     DAXLDT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.257"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1616
                     /locus_tag="ACF2SP_003010"
     CDS             <1..1616
                     /locus_tag="ACF2SP_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFD2360768.1"
                     /translation="IPGDYNLILLDEVLKNEKLKMINCCNELNAGYAADGYARVKGVS
                     ALFVTYSVGGLSTVNAVAGAYAENLPILVISGGPNTNSIQDAEILHHTLATEDNLYVR
                     DIFSRITAHVVCIRKPEQAAMQIDEAIAIAIAKKKPVYIEIACNIANYPISKPTQRAF
                     NTYKISDSSSLAAAVEHAAEKLNSALKPSLIVGSKVRHCNAINSVSELAKCTGYAMAA
                     MPDAKGFISEQHPNFIGIYWGPVSSSGCAEIIDSSDAYLLIGPNENDYTTVGYVWGIN
                     PHKSIKTTKGGVIIGETVYTNIFMNDFLRELSKKLKFNDASYKAYKRIAEETVVYPEL
                     DQPDAPLSSRYLFGQIQKMLSDKKALLAETGDSWFNCMRLSLPEGCPFEIQMQYGSIG
                     WSVGALLGMQAALHNQKRVIACIGDGSFQMTAQEISTMIRYGYKPIIFLMNNACYTIE
                     VQIHDGPYNVINNWRYAELVNVFRGEQSNAKSFVVKTNKELLTAIKQAEKTDALCFIE
                     VFLDKDDCNKNLLEWGSRVANYNSRPPRR"
     gene            complement(1674..2870)
                     /gene="lapA"
                     /locus_tag="ACF2SP_003011"
     CDS             complement(1674..2870)
                     /gene="lapA"
                     /locus_tag="ACF2SP_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase LapA"
                     /protein_id="HFD2360769.1"
                     /translation="MLEYLTWITANIVLVSNSIFGTPHPVHAQLQVPQCLAAKMTTAH
                     DVLAENEQFKIIDLPGSDIENVTLLAEQVNCGDFIDVTNKFSDTLLAAKRKSAENILQ
                     SSALSALKKSQENKNFYEIKHQKEVNAAIKEVVADNIWQTLNHLTSYSNRSATKDTGV
                     AAANWLKSQVDAMATEYDRSDTATFFVKAGWYKQPSVVTVIGKELKTPAIVIGAPMDT
                     LDGHMPGADEGSGAAAIMESTRVLLASKLKFKHPIYIIWYAASDRNLAGSQYVVQYFQ
                     EKSIPVKAVLQLNKTGFRANSDDSTMWVFTDNTNNDLNQYIAKLIKNYIRTPVNFSQC
                     NYECGDHISWSQEDIPVTYAVESDFKNLNPYIGSSSDTMDFLNLEHIANFSKLAIAFS
                     IELGSK"
BASE COUNT          907 a          577 c          627 g          940 t
ORIGIN      
        1 ctataccggg tgattacaat cttattttac tcgatgaagt cctgaaaaac gaaaaattaa
       61 aaatgattaa ctgctgtaat gagttgaatg caggatacgc tgctgatggc tatgcccggg
      121 taaaaggggt ctccgcttta ttcgttactt atagtgtggg tggacttagt actgttaatg
      181 cagttgcagg agcgtatgcc gaaaatctcc ccattttggt tatatcgggt ggcccaaata
      241 ctaattccat acaggatgct gaaattctcc atcacacgct ggcgactgaa gataatttat
      301 atgtaagaga cattttttct cgaattactg cccatgtcgt ttgtattcga aagccagagc
      361 aggctgctat gcagatagac gaggctatag caatcgcaat cgctaaaaaa aagccggtct
      421 atattgaaat agcatgtaat attgctaact atcctatctc caagcctact caacgcgcat
      481 tcaacaccta taaaattagc gacagttcgt ctctggctgc agcagtagag catgctgctg
      541 aaaaattaaa ctccgctcta aagcccagct tgattgtagg tagtaaggtt aggcattgta
      601 atgcaatcaa ttctgtcagc gagttggcaa agtgtactgg atatgctatg gcggctatgc
      661 ccgatgcgaa aggatttatc tcagaacagc atcccaactt tataggtatc tattggggac
      721 cagtcagctc ttctggatgc gccgaaatta tcgattccag tgatgcctat ctgcttattg
      781 ggcctaatga gaatgactat actacagttg ggtatgtatg gggaatcaac ccccacaaat
      841 caataaagac caccaaggga ggggttatca ttggtgaaac agtttatact aatatcttca
      901 tgaatgattt tctcagagaa ttaagtaaaa aattaaaatt taatgatgct tcatacaaag
      961 cttataaacg aattgctgaa gaaactgtcg tttatccaga gttagatcaa ccagacgctc
     1021 cattaagcag tcgttatttg tttggccaaa ttcagaaaat gctttctgac aaaaaagctt
     1081 tgttggctga aacaggggac tcctggttta attgtatgcg cctgtctttg cctgaaggtt
     1141 gtcctttcga aattcaaatg cagtatggtt ctattggttg gtcagtaggt gctttgctag
     1201 gcatgcaagc tgctttgcat aatcaaaaaa gagtgattgc ctgcatagga gatggctcat
     1261 ttcaaatgac tgcccaggaa atatcaacca tgatacgtta cgggtataaa ccaattattt
     1321 ttttaatgaa taatgcttgt tacactattg aagtacaaat tcatgatggt ccttataatg
     1381 tcattaataa ttggcgttac gctgaactgg ttaatgtatt ccgcggggaa cagagtaatg
     1441 caaaatcttt tgtagttaaa accaacaagg aactactcac tgcaattaaa caggcagaaa
     1501 aaacagacgc gctttgtttt attgaagtgt tcctggacaa ggatgattgc aataaaaact
     1561 tgttggaatg gggctcaaga gtggcaaatt ataatagccg tcctcctcgt agatagatct
     1621 tgggtaggaa ttataagaat cgcctaccct gatattaatg atttgacgtg tttttatttg
     1681 gagcccagct ctattgagaa ggcaatggcc agctttgaaa agttagctat atgttccaga
     1741 ttcaaaaaat ccattgtatc tgaagagctg ccgatatagg ggttaaggtt tttaaagtca
     1801 gattcaactg cataggttac gggtatatct tcttgactcc aggaaatatg atcaccgcat
     1861 tcgtaattac actgcgaaaa attaactggt gtacggatgt aattttttat taattttgca
     1921 atatattggt tcagatcatt gtttgtattg tcggtaaata cccacatggt tgaatcatct
     1981 gagttagcgc gaaaccctgt cttgttgagt tgcagtacgg ctttaacagg aatagatttt
     2041 tcttgaaaat attggactac atattgagac ccagccaaat ttctatcgct ggcagcgtac
     2101 cagataatat aaatcggatg cttgaatttc agcttggagg ctaacagaac acgagtgctt
     2161 tccataatgg cagcagcacc gctaccttca tcagcgccag gcatgtgccc atctaaagta
     2221 tccattggag ctccaatgac tatagcaggt gttttcagtt ctttaccaat gactgtgaca
     2281 acggaaggtt gcttatacca accagctttc acaaaaaatg tggcagtatc gctgcgatcg
     2341 tactccgttg ccattgcatc cacctgggat tttaaccaat tggcggcagc tactcctgtg
     2401 tccttggttg ctgaacgatt gctataggag gtcaagtgat ttaatgtttg ccaaatgtta
     2461 tcagcaacta cctcttttat tgcagcattg acttcctttt ggtgtttaat ctcataaaag
     2521 ttcttgtttt cctgagattt tttaagggca cttaatgcac tgctttgcag aatattttcc
     2581 gctgactttc ttttggcagc caacagagta tccgagaact tattcgttac gtcgataaaa
     2641 tcgccacaat tcacttgttc tgctaaaaga gttacattct caatatcgct gccagggaga
     2701 tcaatgattt tgaattgctc attttcagcc aggacatcat gcgcagtcgt catttttgca
     2761 gcaaggcatt gaggtacttg taattgagca tgaactggat gcggtgttcc gaaaattgaa
     2821 ttactcacta atacaatatt tgcggtaatc catgtcaaat attcaagcat ttacactcct
     2881 aaacaactgt agaatccgta tcgattcatg ggcaaagcac attattccaa gtattaaaaa
     2941 ataattttgg agagttcgtt attttttcct atatattgta aatgaccaat ttactggtca
     3001 tgtgttttat ctccaaaata gcgtggacaa atatactaca aaattaacaa a
//
LOCUS       DAXLDT010000257         2953 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXLDT010000257 DAXLDT010000000
VERSION     DAXLDT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.258"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(178..2169)
                     /locus_tag="ACF2SP_003012"
     CDS             complement(178..2169)
                     /locus_tag="ACF2SP_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HFD2360770.1"
                     /translation="MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKA
                     IVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQEL
                     LIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
                     TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIR
                     SIRDNLRKSISEIIIDDQISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQI
                     ETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATALNTNLEAADEIARQ
                     LRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQR
                     LRLSLGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMAT
                     FIMNEKRNFIRDIEKRHGVSVVVIANPYMHPPQYTITRLKEDNVGKSKKPSYSLIQQP
                     EIHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHTVTQEPG
                     KKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAATSAHQQRK
                     KSNPNQQSHGGQQSNRSIPAKNEGNKKREAILAKEPEEK"
BASE COUNT          772 a          572 c          599 g         1010 t
ORIGIN      
        1 actcaaatga tcaggcttat aaacagaaga atttgcatat cccgcgaata gttacaattt
       61 taaaatcggt atcctgacaa gaaagagtgc tgtgttttat ccgcacaatc cagaggtaac
      121 tggactgtac ggataaacgc ttattgaaaa ggtgaatagg caaataatta atatgcccta
      181 tttttcctct ggctctttag ccagtatggc ttcccttttt ttgttgcctt cattttttgc
      241 aggaattgaa cgattgcttt gttggccacc atgactttgc tgattcgggt tggatttttt
      301 tcgttgctgg tgtgcgctgg ttgcagcatt tgtatttccc gatgaagaac gttgctggcc
      361 tcctcctgaa cgacgtctac gatttgcgtt gctgccttgt tgacgtttgt ctcctcctga
      421 gcttttggaa gatgggtgtt tgccttgatt ttgagcacga tgatgttttt taccgggttc
      481 ttgagttacg gtgtgggcaa ctgaatgacc accaaataaa ctggtccata atcgtttaat
      541 gaaactggtt tgagggtgtt tgatgaccct gtgttcggca aatgatttca cagcaggctc
      601 atcatgtgac acgggttctt tatcactgct gggaacatgt atttcaggtt gctgaatcaa
      661 agagtaactt ggttttttag atttgcctac gttgtcttct ttcagacgag taatcgtata
      721 ttgtggaggg tgcatataag gattcgcaat aacaacgaca ctgacaccat gccttttttc
      781 aatgtccctg ataaaattac gtttttcatt cataatgaac gtagccattt ctggcggcaa
      841 ttgcacttga atctcagctg ttttttcctt gagcgcttct tcttcaataa ggcgggtaat
      901 tgataagcca tgggactgta tactgcgtac agttcctcta ccttcacaac gtggacaaat
      961 ttcctgggcg gtttctccta atgataaccg gaggcgttgc cgtgacattt caagaaggcc
     1021 aaagcgtgaa attcgcccta cttgaattct ggctctatca gcctgcagtg cttctttgag
     1081 tcgattttct acctctcttt gatttttact agaactcatg tcaataaaat caataactac
     1141 caaaccacct aaatcacgca ggcgaagctg gcgagcaatt tcatcagctg cttcaaggtt
     1201 ggtatttaat gcagtcgctt cgatgtcggc accgcctgta gctttggccg agttaatatc
     1261 aattgatacc agagcttcag ttctatctat gaccaaggca cctccagaag gaagcatgac
     1321 ctggcgttga taagcggtct caatttggct ttcaatttga tagaaattaa aaagagggat
     1381 gctgctgtta tagagtttta gattaggtaa aaattctggt tttacgcgtt caatgtattg
     1441 ttttgctttg atataggata tttgatcatc aataataatt tcactgatgg attttcgcaa
     1501 attgtcacga atggaacgaa taatgacatc gccctcttga tgaatcaggc aaggtgccag
     1561 ctccgaatta taagcttcct tgatggattg ccactgattg caaagcatgt ttaagtcatc
     1621 ttgcagttct tccagacttt ttccaacacc tgcagtgcga ataataagac ccatgtcttc
     1681 agggagggtt aaagcattta atgtttcacg tagttcatca cgctcatcac cttcaatacg
     1741 tcttgatatt ccgcccgaat gagggttatt gggcattaaa actaggtagc aacctgccag
     1801 ggtaataaaa gttgtcagtg ctgctccttt attccctctt tcttctttat ccacttggat
     1861 aagtaattct tgaccttcac ggatgaggct ggtaattgga ggtttctcat ctccaaattc
     1921 ttccggattc ttgcttaaat attcaggcgc tatttctttt aggggtaaga aaccttggcg
     1981 tttggaacca tattcaacga aaacagcatc aaggctgggt tctcttcttg ttactatggc
     2041 tttataaata ttaccttttt tctttatttc tcccgggcat tcaatgtcca gatcaaataa
     2101 ttgattgttt ttaattagtg caacacgaat ttcttctgtt tgcattgcat tgattagcat
     2161 tttttccatg tataacccca tatttagggc gcatgcattt acatcgagat agtgtaaggc
     2221 tcagtatagc tgtatgatca tttctacagt tttcttgcca tgaagttaat tttccgggtc
     2281 ccatatcttg cattcgtctt gaatctgcag agagagggag tcagaaaatt aatatcctgg
     2341 tctctataat tctggtgaaa tgataacagc ctattcccta attgctcaat tttgtttttg
     2401 aaaaacaatg ttattaagct ggcagggtga ggaactttaa gctgacccat gaccttgtgg
     2461 acagaccgta cgcatgttac ctcagtagtt aaaggtgcac actgtatgat gtaccgttta
     2521 cagattaagt gggttggaaa aaaaaggcct ttcatcaggc gtgcaaaagc ccggcaataa
     2581 ttcgtaattg gtataaagca tgtctggcat gcgtaggtat ctgtcgaaat aattatcaat
     2641 tttactctgg tattgggtcg cttccttata tcttcgactt aaatggaagc gcattttgtt
     2701 ttttcttcca agcatctgct caggcgtatt acgatgaaaa aacagagatg attctttaac
     2761 ttgtgattca agttcttatt gaattcaacc tgttttatat tcgcttgagc aactgcttct
     2821 ttagttcgat ttgctatcaa attaactaaa tttatgctat tctgttaatt tttcattctt
     2881 cattaatgat gaaaaattaa gatgaatgca agtattttaa ctaattaatt acaaatcttt
     2941 tatcgattat gaa
//
LOCUS       DAXLDT010000258         2937 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXLDT010000258 DAXLDT010000000
VERSION     DAXLDT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.259"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..290)
                     /locus_tag="ACF2SP_003013"
     CDS             complement(<1..290)
                     /locus_tag="ACF2SP_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360771.1"
                     /translation="MNKYGGAIILLSFPHQLIHALAALFTDREQRRINKDAPILIFIW
                     SYRWFDHKKNSPFLEIIKKLSKIENCQLYIPSLLQRVFGLSEFRALKQRVKYV"
     gene            complement(296..1720)
                     /locus_tag="ACF2SP_003014"
     CDS             complement(296..1720)
                     /locus_tag="ACF2SP_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HFD2360772.1"
                     /translation="MSWSIRIENLSKQYRIGSTTTTAAELINEKLRKFLLLLDFKKRN
                     RFSSDKPLPSSIAQEGRTVVDASQISPEYPNHFWALRDINLEIEAGERLGIVGQNGCG
                     KSTLLKILSRIVSPTEGLFRFRGRLISLLEIGTGFHGELTGRENIFLNGTIMGMKTHE
                     IKNRLNQIIEFSELGSMIDTPVKRYSSGMYIRLAFAVAAHLESEILIVDEVLAVGDAA
                     FQRKCTEKMLDLANQGRTLLFVSHDMETVNRICNRAIRMSHGRIVGDSKFDTSAASKS
                     SVMDITRDYLRSGAKYKSEQVWPSDKPMIFLNSLKLNRVRLIDYDNKVRSNFNLNETI
                     VVEIDFTIIKETYPFNIHLYLKDSSGRVIFVSMDNHAWQSKKVRPLGNYTERCTLYSP
                     LLNIGDYHIDIEFWPGNALENRLVVNSVVSFELKDGAVNEGVRGNWASDWPNSLIRPQ
                     LDWSISTNKKQHLVLKEHELVTEK"
     gene            complement(1717..2568)
                     /locus_tag="ACF2SP_003015"
     CDS             complement(1717..2568)
                     /locus_tag="ACF2SP_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD2360773.1"
                     /translation="MTSISSKTQTFKETVVYGVDKFRLLAVLRNIWDYRLLLWIFCKR
                     DLKGRYSQTILGLGWVILTPLITVGVFVIVFGIMIKVPTDGLPTVMFYLVAVIPWYSF
                     LNVLNPSIQMIEGNASLITKVYFPRALIGGAYAMGAAVDFLMAYVFLITPFAIYYGLW
                     SIKLLIIMPFLLISTLMIGLGIGLVLAPINAKYRDIKHFMPLALQLFYYSTPAIYPVS
                     AVPVWAKPWYAVNPLSLVITSYREVLMGHWPSPTIIMTLSGMAIITFLVGLVAFHHME
                     HKVVDIL"
     gene            complement(2534..>2937)
                     /locus_tag="ACF2SP_003016"
     CDS             complement(2534..>2937)
                     /locus_tag="ACF2SP_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360774.1"
                     /translation="QFISYDDTIQLITCCDLFVACVSSTLRWAIAKGIPAINYDCYQY
                     GYTDFDAKGVFNVNDFKSFSAVLEELTRDRQKYEGAKQNQQECASAWGILDGNSQKRI
                     VNLINKLVQSESVIDIENKNYDLNILKNSDV"
BASE COUNT          975 a          606 c          471 g          885 t
ORIGIN      
        1 acgtatttta ctcgttgctt taatgcccga aactcagata atccaaacac tctttgaagc
       61 aaactgggaa tataaagctg gcaattttct attttcgata atttcttaat tatctcaaga
      121 aatggtgaat tctttttatg atcaaaccaa cggtatgacc agataaatat caaaattgga
      181 gcatctttat taattctgcg ctgctctcta tcagtaaata atgctgcaag agcatgaatt
      241 aattgatggg gaaaagagag taaaatgatt gcaccaccat atttgttcat gttgattact
      301 tttcagtgac taattcatgc tcttttaaaa caagatgctg tttcttattt gtcgaaatac
      361 tccaatccaa ctgtggtcta atcaatgagt ttggccaatc agaagcccaa ttgcctctta
      421 ccccctcatt tacagctcca tctttcaact cgaagctgac tactgaattg actaccaatc
      481 gattttccaa agcattacct ggccaaaatt caatatcaat atgataatcg ccaatgttta
      541 ataagggtga atacaaagta catcgttcgg tataatttcc caatggacgt actttcttag
      601 attgccaggc atgattatcc attgacacaa atatcacacg cccagagctg tctttaagat
      661 aaagatgaat attaaaaggg tatgtttctt taataattgt aaaatctatt tcaacaacaa
      721 tagtttcatt taaattaaaa ttagaacgaa ctttattgtc gtaatctatc aatctaactc
      781 ggttaagctt aagggaattt aaaaatatca ttggcttatc actaggccaa acctgttccg
      841 atttatattt cgctcctgag cggagatagt cgcgagtgat atccatgact gaacttttgc
      901 ttgctgcact tgtatcaaat ttactgtcac cgacaattct gccatgcgac attcgtatgg
      961 ctctattaca aattcgattc acggtttcca tatcgtgact gacaaataat aaagttcttc
     1021 cctgatttgc taaatccagc attttttccg tacacttgcg ttgaaatgct gcatcaccta
     1081 ccgctaacac ttcatcaact ataaggattt cagactccaa atgagcagct acagcaaatg
     1141 ccaagcgtat atacattccg gaagagtagc gcttgacagg agtatctatc attgacccca
     1201 attcagaaaa ttcaataatt tgattgagtc gatttttaat ttcatgggtt ttcattccca
     1261 taatagtgcc atttaaaaaa atattttcac gtccagtcag ttcaccatga aatcctgtac
     1321 cgatttccaa caaactgatt aagcgccccc taaatcggaa taacccttcg gtaggagaaa
     1381 caatacgtga tagaattttt aataatgtgc tcttaccgca accattttgt cctactatac
     1441 caagccgctc ccctgcctcg atttcaagat taatatcccg caaagcccag aaatgattgg
     1501 gatattccgg gctaatttga cttgcatcga ctacagttct accttcttgt gcaatggatg
     1561 aaggcagtgg cttatcggat gaaaatcgat tccttttttt aaaatccagt aataaaagaa
     1621 attttctcaa tttttcatta atcagctctg ctgcggttgt agtagtacta ccaatccgat
     1681 attgtttgga aaggttttca atacgaatag accaactcat aaaatatcaa ctaccttatg
     1741 ctccatgtga tgaaatgcaa caagccccac aaggaaagtt ataattgcca taccagataa
     1801 tgtcattatt atagtaggtg atggccaatg ccccattaac acctctcgat aactggtgat
     1861 aacaagagat aatggattaa cagcatacca tggtttagcc cacacaggca cagcagaaac
     1921 agggtaaatc gctggagtgg agtaataaaa taactgcaaa gccagaggca taaaatgttt
     1981 aatatcccta tattttgcat tgataggtgc taaaaccaaa ccaataccta atccaatcat
     2041 taaggtagat atcagcagga atggcataat aattaataac ttgatagacc aaaggccata
     2101 ataaattgca aagggggtta tcaaaaacac ataagccatc agaaaatcaa ccgcagctcc
     2161 catagcatag gcaccgccta tcaacgctct tggaaaatag acctttgtta ttaacgaagc
     2221 atttccctca atcatttgta tggaagggtt gagaacattt aaaaaggaat accaaggtat
     2281 aaccgcaacc aaatagaaca tgacagtagg cagcccatcg gtaggtacct ttatcatgat
     2341 gccaaaaaca atgacaaaca ccccaaccgt aatcaaagga gttaaaataa cccaacccaa
     2401 tcccaagatg gtttgactgt aacgcccttt gagatctcgt ttacaaaaaa tccataaaag
     2461 caagcggtaa tcccatatat ttcttaaaac agctaaaagc ctaaacttat caactccata
     2521 gactactgtt tctttaaacg tctgagtttt tgaggatatt gaggtcataa tttttgtttt
     2581 ctatatcaat tacagattcc gattgaacta acttattaat taggttcact attctttttt
     2641 gggagttccc atccaggatg ccccatgcac tagcgcattc ttgttggttc tgtttagcac
     2701 cctcatattt ctgcctatca cgcgttaatt cttccaaaac agcagaaaag cttttaaagt
     2761 catttacatt aaaaacacct tttgcatcaa agtctgtata tccatattga taacaatcat
     2821 aattaatggc agggattcct tttgcaattg cccaacgcaa agttgatgaa acgcaagcta
     2881 caaataaatc acaacatgta ataagctgga ttgtatcatc ataggatata aattgtt
//
LOCUS       DAXLDT010000259         2915 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXLDT010000259 DAXLDT010000000
VERSION     DAXLDT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.260"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..825
                     /locus_tag="ACF2SP_003017"
     CDS             64..825
                     /locus_tag="ACF2SP_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2360775.1"
                     /translation="MKRIVFILIFISNVLVHSESLKVGVLQFAPPFSSISDKANHYYG
                     FVVALMNIICNRLQEKCEYIPIPKEGELKGLDKGVYHIVFSSTPITASLPANYRYSLP
                     YLPSDGQFLTLKNNSIDTLNDINFKKVGFFKINFQQSPLLKKYETTNTLIEYTDPAEL
                     VNALTTKKIDAILINYQASRFIVNNLNPTEDDQLKLIGDKIPIGSGYAIIALKNQSAL
                     IDKINHILLEMEKDGSYLRIYNEYFGANPAYNLHH"
     gene            complement(951..2279)
                     /locus_tag="ACF2SP_003018"
     CDS             complement(951..2279)
                     /locus_tag="ACF2SP_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFD2360776.1"
                     /translation="MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKM
                     NHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIES
                     AYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
                     NGNWILSIAAYDAGEGAIGRAVKANNTNGGRVSFWNLDVPKETQIYVPRFLALAEIMS
                     NPRAYKINLPAMPFRPYFEEVNIGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPY
                     KPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKL
                     NRLSDHSIRPNQILLIPSAKSTPSLPFHEISKSKFLSQTPTKGKVYRVIHIVQANETY
                     NTLERLYKVTAQDIMKWNHIGSGEALVKGRQLIIWKKSSNLEWNRTFKKP"
     gene            2639..>2915
                     /locus_tag="ACF2SP_003019"
     CDS             2639..>2915
                     /locus_tag="ACF2SP_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360777.1"
                     /translation="MGTFFEWFPREVGLDKNPDAFRTDCYQYYWGEPPRWGGTVDACE
                     VETDLYAMVYNSIGLLSYLIPLVGGYLALHFARNANSKAFSEDNPEPG"
BASE COUNT          894 a          557 c          538 g          926 t
ORIGIN      
        1 ctataattaa aaaagattac ttctcttatt aaaaatggac cagattaaga agagacccaa
       61 ttcatgaaac ggatagtatt tatactgatt tttataagca atgttctagt tcatagtgaa
      121 tcactcaaag tgggtgtctt acaatttgca cctcctttct catctatatc agacaaagcg
      181 aatcactatt atggtttcgt tgtcgccctc atgaatataa tatgcaaccg tttgcaagaa
      241 aaatgcgaat acatccctat tcctaaagaa ggcgagttaa aaggcctgga taaaggtgtt
      301 tatcatattg tttttagctc aaccccaatc acggcatcat tacctgccaa ttatcggtac
      361 agcttgccct acttacccag cgacggtcaa tttctgacat taaaaaacaa ctcgattgat
      421 accttgaacg atatcaattt taaaaaagtt ggtttcttta agattaattt tcagcaatct
      481 cctcttttaa aaaaatacga aaccaccaat acgcttatag agtacacaga tccggcagaa
      541 ctagttaatg cactaacaac caaaaaaatt gatgcgattt taatcaatta tcaagcgtca
      601 cgattcatcg tgaataatct caaccccact gaagacgatc aattaaaatt gattggcgat
      661 aaaataccga taggaagcgg atatgcgatc atagccctta aaaaccaatc ggcattgatt
      721 gataaaatta atcacatttt gctggaaatg gaaaaagatg gttcctacct ccgtatctac
      781 aacgaatatt ttggtgcaaa cccggcatat aatcttcatc attagttggt tagtgtgaaa
      841 atcgccacat atgagaaatt taaccaaatt tattaataag ttattgaata gttaagcgtg
      901 aaattttgac cgaacacggg ttatgtttag aatgcaggat tgccggaagc ttaaggtttt
      961 ttgaaagtcc tgttccattc aagattgctt gactttttcc aaataattaa ctgtcgccct
     1021 tttaccaacg cttctcccga gccaatatga ttccatttca taatatcctg agctgtaact
     1081 ttataaagtc gctcaagggt attatacgtt tcattggctt ggacaatatg tatgacccgg
     1141 tatactttac cttttgttgg tgtctggctt aagaattttg actttgaaat ttcgtggaaa
     1201 ggcaatgatg gagtactttt ggcgctcgga attaacaaga tctgattggg tcttatcgag
     1261 tgatctgaca gacgatttaa tttttttatt aatgaaacag tcgtatgata ttttctggca
     1321 atactttgta aactatcact cggaaggacg agatgttttc tccagctcac tcgtttatca
     1381 acaggtgaat gggataaatt gaaataaaat tgagtcactt ttgctatagg gataagcaag
     1441 gtgaagggtt tatagggtgc ggtagcccat ctattataac ccggatttaa tttggttaat
     1501 tcactaaccg aaattcctgc taaactagcc gcatgattca agtcaatttg actgccaata
     1561 ttcacctctt caaaataagg tcgaaatggc attgcaggaa gatttatttt ataagcccga
     1621 gggttgctca tgatttcagc cagagctaaa aatcgaggaa cataaatttg agtttcttta
     1681 ggtacatcca gattccaaaa actgacccta cctccattag tgttatttgc ttttacagct
     1741 ctgcctatgg ctccttcgcc agcatcgtaa gctgcgatgg ataatatcca gtttccgtta
     1801 aacactttat ttaaatgcat cagatgagtc agggctgcat cagttgaatg attaatgctt
     1861 cgtcttccat caaaccacca atcctgaatt aaacctaatt gattccccgt caccggcatt
     1921 atttgccata aacctgcagc accaactttt gagtaagcaa aaggatcgta agcgctttct
     1981 atcataggga gtaatgccaa ctcaccgggt aaatttcttc tttgtatttc tcgagttaca
     2041 tgataaagat aaggctcagc ctgtttacag gccttagtca caaaccctgg atgatccagt
     2101 agccagcgaa tttgatcttg aacctctggg cgataggctt catgattcat cttgaattca
     2161 ctgcgcaaaa catcccatac atcaggtgtt tcaaatgatt tagcatgagt actgaagata
     2221 aaaaaaataa ggcaactgaa tagcagctgc atttttttat cgatgaatgt cttaatcaaa
     2281 ttactattcc tgctctataa agcttctctg gagcccgttt ataatgtatt gtttctcttc
     2341 gcctgaaccg gccctgagcg cgtcttataa aatatactcc tcttagtatc ttgtataaag
     2401 cacttcctgc aatataaggc catgataatt taacaccctg tctatttaac gttttttatt
     2461 aatattgctc taacatttgt ttcgatatat gctgatggct cctccagcaa ttatttatga
     2521 cagactgatt tttatcagag cggttagggt ttattttcat atggaaatgg gatggctggt
     2581 tgtgtgtatc aagcatcaag gaatgataag aattaatata ttcaggagat tggaaattat
     2641 gggcactttt tttgaatggt ttcccagaga agtaggttta gataagaacc cggatgcgtt
     2701 taggacagat tgctatcaat actattgggg agagcctcct cgatggggcg gcaccgttga
     2761 cgcatgtgaa gttgaaactg atttatacgc tatggtatat aattccatag gacttttgag
     2821 ctacttgata cctttggtag gtggatattt agcattacat tttgcacgaa atgcaaactc
     2881 caaagcattt tccgaggata atccagaacc tggcc
//
LOCUS       DAXLDT010000260         2875 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXLDT010000260 DAXLDT010000000
VERSION     DAXLDT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.261"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            89..307
                     /locus_tag="ACF2SP_003020"
     CDS             89..307
                     /locus_tag="ACF2SP_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360778.1"
                     /translation="MELLDAVEALVKNPLLDTVALEDWVLSKSLVESYKNSDTHKIRE
                     LITDKKNFSDMSHVIPYPDMSHVFPVTK"
     gene            316..1086
                     /locus_tag="ACF2SP_003021"
     CDS             316..1086
                     /locus_tag="ACF2SP_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFD2360779.1"
                     /translation="MKVVALVGTGIKTIAHITEEAKGYISTCDKVIYLVNEPLLERYI
                     RKLAKSSESLDEIYFNSKSRESAYNNIAQYIYSAIEDVRSLCVVIYGHPCVFATPGLL
                     ALSMLDDSIKTVVCPGISTQDCLYADLRFDPASGGIQSFDATEYLLYDKVVDPSSHVV
                     IYQIGMVGNLGLPTNKINFEALNFIKKKLIELYKKEKKAVIYEAALYPGTHPKISEFD
                     LEQLDRQELTTLSTLYIPPDKIQRMPSSDALRLLNQVA"
     gene            1130..2335
                     /locus_tag="ACF2SP_003022"
     CDS             1130..2335
                     /locus_tag="ACF2SP_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360780.1"
                     /translation="MSSTVSSSKVGFSFSDLPVTCKLLLALSFIEAIGGGLSYHIAYF
                     FSVSTNFNKLNIGFLGFSMGIGAILGSICGGYVTGKAQAKGIIGASFLLMGVAFVILA
                     KVSSFHLSVVFVLLMGFGINLFITCSNSSFLQISKNTNHSLTIAQSYKNALENTGGIV
                     AMVLIMLLAQNHFKLAMTLVGVTLILFSIVIFLKLEISNQDVAAKPSNKEEIESVYSS
                     LIPMLFSVFCIGLTYGIQKTVLGIHLNETIGNSLIIGFFFATDPILIALFQVKLSKRI
                     ERFNKHIVACIGCILLGVSTFAMGLSSTIQGMFCSLLVFTLGEMLFMAYSVALCHQYG
                     SSNHSGLGIGAWRSAYALGMMIGPLISGVAMYYFNAQSAWTISSLLCFMSAFLVFNSK
                     RAQRLEAVY"
     gene            complement(2318..>2875)
                     /locus_tag="ACF2SP_003023"
     CDS             complement(2318..>2875)
                     /locus_tag="ACF2SP_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360781.1"
                     /translation="VAFDKSNLERDFFIQDTAKYIFEILIGEKYGFSFTNTDEFLPAS
                     WIGYVLKDIYKLKTEPSILIPKKHVEKNPVYFSLQHPIFRPYQGLSRKRATLISELEN
                     MKIVTNKYKELFSSNHEEWAGTILEEKAKKAQFVYYHGFENKKNWLSDIHHLLKNDSG
                     VHHLSNDTFCQDAPFFKGCISINSF"
BASE COUNT          892 a          437 c          540 g         1006 t
ORIGIN      
        1 gataatgatt gatttttgat aggataatga tttaaatttt tataaaataa atagtgcaaa
       61 attttgattg ccaaattttg gagggactat ggaattatta gatgcagttg aggcattggt
      121 gaaaaatcca ctccttgata cagtggcttt agaggattgg gttttaagta aaagcttggt
      181 tgaatcatat aaaaactcag atacacataa gatcagggag ttaatcactg ataaaaagaa
      241 tttttcggat atgagccatg tgatcccgta tccggatatg agccatgttt ttccagtgac
      301 caaataagga tttaagtgaa agttgtagcg ttagttggta ctggaattaa aacaatagct
      361 catattacag aagaagcaaa aggatatatt tctacttgtg ataaggtaat ctacttagtt
      421 aatgaaccct tgctagagcg ctatattcgt aaattagcga aatcatcaga gtcactggat
      481 gaaatatact ttaactctaa atcaagagaa agtgcttata ataatattgc acaatatatt
      541 tatagcgcga tagaagatgt acgatcatta tgtgttgtta tatatggtca tccatgtgtg
      601 tttgctactc ctggcttgct cgctttgtct atgcttgatg atagcattaa aacagtggtg
      661 tgccctggta tttcaactca agattgttta tatgccgatc ttaggtttga tcccgcatct
      721 ggaggtatcc aatcctttga tgctacggaa tatcttctat atgataaagt agtagatcct
      781 tcttctcatg tagtaattta ccagattggt atggttggaa atctgggttt accaacaaat
      841 aaaatcaatt ttgaggctct taattttatt aaaaaaaaat taatcgaact ctataaaaaa
      901 gaaaaaaaag cggttattta cgaagccgct ctttatcctg gtactcatcc aaagatatct
      961 gaatttgatt tagagcaact tgataggcaa gagttaacaa cactgtctac tttgtatatt
     1021 cctcccgata aaatacaacg aatgccgagt tctgatgcat taaggttatt aaatcaagtg
     1081 gcatagttta gtggatgtat ctaattttta tttaatgtga gggattacaa tgagctcaac
     1141 agtcagctct tctaaagtag ggttctcttt tagtgattta ccagtcactt gtaaattatt
     1201 attggctctt tcttttatag aggctattgg aggcggccta tcttaccata ttgcgtattt
     1261 ttttagtgta agcaccaatt ttaataaatt aaatataggc ttcttaggct tttcaatggg
     1321 gattggagct attttgggca gtatatgtgg tggatacgtt acgggaaaag cacaagcaaa
     1381 gggtataatt ggggcaagtt tcttactcat gggcgtcgca tttgttattc tagcaaaagt
     1441 gagttctttt catttatctg ttgtctttgt attgttaatg ggatttggga tcaacttgtt
     1501 catcacttgc agtaactcat ctttcttgca gatttcaaaa aataccaatc acagtttaac
     1561 gattgcccaa tcctataaaa atgcactgga aaatactggt ggtattgtgg caatggtttt
     1621 gataatgctt ttagcgcaaa atcattttaa attagcaatg actttggtcg gcgttacttt
     1681 gatattgttt tcaatagtta tttttctaaa attagaaatc agtaatcagg atgtggccgc
     1741 aaaacccagt aataaagaag aaattgagtc agtctatagc tcgcttattc caatgttatt
     1801 ttcagtattt tgcatcggtt taacttatgg gatacaaaaa acagtactag gcattcactt
     1861 aaacgaaaca ataggaaatt ctttaatcat tggatttttt ttcgcaactg atcccatttt
     1921 aatcgcatta tttcaagtaa aattatcaaa aaggattgag cgattcaata aacatatagt
     1981 tgcttgtatc ggctgtattt tattaggggt gtcaacgttt gctatgggcc tgtcttctac
     2041 aatacaagga atgttttgtt cgcttttagt atttactctt ggtgaaatgt tgtttatggc
     2101 gtactccgtt gcattatgtc atcaatatgg aagctctaac cacagtggcc taggtatagg
     2161 ggcgtggcgc agcgcatatg cattaggcat gatgattggc cctctcatat caggagtagc
     2221 aatgtattat ttcaatgccc aaagtgcttg gacaatatct tctttgcttt gtttcatgag
     2281 tgccttctta gtatttaatt ctaaaagagc acagcgatta gaagctgttt attgagatgc
     2341 atcctttaaa aaaaggcgca tcttgacaaa aagtgtcatt gcttaaatga tgcactccag
     2401 aatcattttt taataaatga tgaatgtccg aaagccaatt ttttttattc tcaaacccat
     2461 ggtaataaac aaattgagct tttttggctt tttcttcaag aattgtacca gcccattctt
     2521 catgattaga ggaaaatagt tctttatatt tatttgtaac gattttcata ttttcaagct
     2581 cagaaattag agtcgctctt tttctggaca acccttgata tggtcgaaaa ataggatgtt
     2641 gaagcgaaaa atatactgga tttttttcaa catgtttttt aggtattaat atagacggtt
     2701 ctgtttttag cttataaatg tcttttaaaa cgtagcctat ccatgatgca ggcaagaact
     2761 catcggtatt ggtaaacgaa aacccatatt tttcacctat gagaatttca aagatatatt
     2821 tagcggtatc ttgaataaaa aaatctcgtt ctaaatttga cttatcaaaa gcaac
//
LOCUS       DAXLDT010000261         2873 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXLDT010000261 DAXLDT010000000
VERSION     DAXLDT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.262"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            17..1309
                     /locus_tag="ACF2SP_003024"
     CDS             17..1309
                     /locus_tag="ACF2SP_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360782.1"
                     /translation="MHTENRTNYAMIGWLLCGLGAIFYSYEYLLRIAPSVMENALRTH
                     FNLSASGFGFLSSIYYLAYVPMQLPVGVLLDRYGPKRLLTMACLVCVIGTYMFTGTTI
                     FWVAATGRFLVGFGSAFAFVGVLKIATLWLPENRLAMISGMTSALGPIGAMLGDNFLE
                     LFIVKMGWVKTLNMTAAFGIVLTLVLWVGIRDRKGQHRQSGTVPTLKKGLVDLGIIIT
                     SRQIWVNGMYGCLVYLPTTVFAELWGIPYLHHAHGLTTQAAGLANSVLFLGFIIGAPI
                     MGYISDRLYRRKFPMLIGASGAAIVMMMILYLPGLNESNIQALMFLLGLLYSAQAIVF
                     AVGRELSPGEAAGTAMAVTNMIVMLGAMFLQPLVGHLLDFSLSTHLSDTTGIVVDNMS
                     KLYTVDDYQFALSIIPLGILIAAILTFFLKETHAKAPK"
     gene            1290..2567
                     /locus_tag="ACF2SP_003025"
     CDS             1290..2567
                     /locus_tag="ACF2SP_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360783.1"
                     /translation="MQKHLNNMTTKKQISYGVLVCFIGAVFYCYEFILRIVPGALQTE
                     LSTALGHISATTFGQISALYYFAYSPMQMPVGMLMDRYGPRRLLTFACLCCALGSWMF
                     TLTWSIVLVGIGRFLVGFGSSFAFVGVLSLALHWLPRRYFSLVAGLITTLGMLGLVYG
                     EIKITEWSEVIGWDHVLMHIAMIGSALCILTFFVVRDGPEGYQPNKYPWPEFFHNVLK
                     VLTSHEVWLIGFVGACLYTSLSVFGELWGKTYLEQAHHLTKVEAAKTVSAVFLGWAVG
                     APLAGYISDITRRRLLPLVIGAIVSLICISFVLYCPGLSYLTLNILLFLYGVFSATEI
                     IVFIMAKECSGAKLSGTVFAVTNMIISLAGAIFQPLVGKLLDTFGDSGIVAGEHIYTV
                     DDYQVALSILPISLLLVTILAFFIKDKDHSNNS"
     gene            complement(2598..>2873)
                     /locus_tag="ACF2SP_003026"
     CDS             complement(2598..>2873)
                     /locus_tag="ACF2SP_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFD2360784.1"
                     /translation="PPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVII
                     SNHDTEFTRHHYKKAQIKSFYVPRWISCRSQMRLPVKELVAVFNCPS"
BASE COUNT          693 a          498 c          666 g         1016 t
ORIGIN      
        1 tgttaaggag ataattttgc atactgaaaa ccgaactaat tatgcaatga taggatggct
       61 actttgtggg ctgggagcaa tattttatag ttatgaatat ttattacgca tcgcaccaag
      121 cgtaatggaa aacgcgttaa ggactcattt taatttatcg gcgtctggtt ttggcttcct
      181 ttcttccatt tattatctag cttatgttcc tatgcaattg ccagttgggg ttttattgga
      241 caggtatggg ccaaaaagac tactaacaat ggcttgcttg gtatgcgtta ttggaacgta
      301 tatgtttacg gggacaacta ttttttgggt agccgcaaca ggtcgttttc tagttggatt
      361 tggctctgct ttcgccttcg tcggtgtttt aaagatcgca actctttggc tgcctgaaaa
      421 tcgattggct atgatctctg gtatgacatc ggcactaggc cccatcggtg ctatgctggg
      481 cgataacttt ctggaattat tcatcgttaa gatgggttgg gttaagacac ttaacatgac
      541 tgcagctttt ggtattgtat tgactttagt tttgtgggtt gggatcaggg acaggaaggg
      601 acagcacagg caaagcggta cagttcccac tcttaagaaa gggttagttg atctgggaat
      661 tattattacc agccgacaga tatgggttaa tggaatgtat ggctgtttag tttatctgcc
      721 aactacggtt tttgcagagt tgtggggtat tccctattta caccatgccc atggtttaac
      781 tacccaagcg gcaggattag ctaactctgt actatttttg ggatttatta ttggtgctcc
      841 aataatggga tatatttcgg atcgtttgta tcgacgtaaa ttcccaatgc taattggtgc
      901 tagcggcgct gcaattgtca tgatgatgat tttatatttg cctggattaa atgaatccaa
      961 tattcaagct ttaatgtttt tattaggatt gctttatagc gctcaagcta tagtttttgc
     1021 tgtgggcaga gagctgagcc ctggtgaggc agcaggaaca gcaatggcgg taactaatat
     1081 gatagttatg ctcggcgcaa tgtttttgca gccgttggtg gggcatttat tagacttcag
     1141 cttatcaact catttaagtg atactactgg tattgttgtg gataacatga gtaaactata
     1201 taccgtagat gattatcagt ttgccttatc cattattcca ttaggtatat tgatagcggc
     1261 cattttaact ttctttctaa aagaaactca tgcaaaagca cctaaataat atgactacta
     1321 aaaaacaaat ttcatatggc gtcctggttt gctttatcgg ggctgttttt tactgttatg
     1381 aatttattct cagaattgtt ccgggagctt tacaaacgga gctaagtaca gctttgggac
     1441 atatttctgc taccaccttt ggacaaattt cagccctgta ttattttgca tattcaccca
     1501 tgcaaatgcc tgtagggatg ttaatggatc gctatgggcc gagacgactt ttgacttttg
     1561 cctgtttgtg ttgtgccttg ggctcgtgga tgtttacttt gacctggtca attgtgctag
     1621 ttggaattgg tcgattcctg gtgggatttg gctcatcctt cgcttttgtt ggtgtcttgt
     1681 ctctggcttt gcattggctg ccacgtcgtt atttttcttt agtggctggt ttgattacta
     1741 cgctaggtat gttaggactg gtttatggtg aaatcaaaat aactgaatgg tcagaggtca
     1801 taggttggga tcatgtttta atgcacattg caatgattgg ttctgcgctg tgtatcttaa
     1861 ctttctttgt tgttagagat ggtccagaag gatatcaacc caacaaatat ccttggcccg
     1921 agttttttca taacgtgctt aaggtactga cgtcccatga agtgtggctt attgggtttg
     1981 ttggagcctg cctttatact tcattatcgg tatttggaga gctgtggggt aaaacctatt
     2041 tggagcaagc acatcattta accaaagtag aagccgcaaa aactgtttct gctgtttttc
     2101 tggggtgggc ggtaggagcc cccttggctg ggtacatatc cgatattacc cgcagacgat
     2161 tgttgccttt ggtgattggt gcgatagttt cattaatatg cattagtttt gttttgtatt
     2221 gccctggcct atcctatctt accttgaaca tattgctatt cttgtatggt gttttcagtg
     2281 caactgaaat tatagttttt attatggcta aggaatgtag tggagccaaa ttatcaggaa
     2341 cggtttttgc agtaaccaat atgattattt ctttggctgg tgctattttt caaccgctcg
     2401 tagggaagtt actggataca tttggcgaca gcggtatagt ggcaggggaa catatctata
     2461 cggttgatga ttatcaagtg gctctttcga tattacctat ttcattactt ttggtaacta
     2521 ttctggcttt ctttattaaa gacaaagacc attccaataa ctcttaatct tgtgataaag
     2581 actcttcttt gtctttgtca ggatggacag ttgaatactg cgactagctc tttaaccggc
     2641 agtcgcatct gacttctgca gcttatccag cgaggtacat agaaactctt tatttgtgct
     2701 tttttgtaat gatgtcgcgt aaattcagta tcatgattcg atataatgac aggaatccct
     2761 ttggaggctg tttcctgggc caattcagcc aactcaattt gatcttctaa agaaaataat
     2821 ttttggttat agggtaatgt ttgattgttt tcggtcagtg gcacataggg tgg
//
LOCUS       DAXLDT010000262         2854 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXLDT010000262 DAXLDT010000000
VERSION     DAXLDT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.263"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..647)
                     /gene="mip"
                     /locus_tag="ACF2SP_003027"
     CDS             complement(<1..647)
                     /gene="mip"
                     /locus_tag="ACF2SP_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrophage infectivity potentiator Mip"
                     /protein_id="HFD2360785.1"
                     /translation="MKMKLVTAAVMGLAMSTAMAATDATSLATDKDKLSYSIGADLGK
                     NFKNQGIDVNPEAMAKGMQDAMSGAQLALTEQQMKDVLNKFQKDLMAKRTAEFNKKAD
                     ENKVKGEAFLTENKNKPGVVVLPSGLQYKVINSGNGVKPGKSDTVTVEYTGRLIDGTV
                     FDSTEKTGKPATFQVSQVIPGWTEALQLMPAGSTWEIYVPSGLAYGPRSVGGPIGP"
     gene            852..2228
                     /locus_tag="ACF2SP_003028"
     CDS             852..2228
                     /locus_tag="ACF2SP_003028"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFD2360786.1"
                     /translation="MNISVFDLFSIGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQ
                     RVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGK
                     KEIPFHEATDFLFLQKELLPKHSNGMRFSAFDGNANLLIEQVYYSIGGGFITTEEDFD
                     KSTTDTNPPPYPFATATELLKLCKKHHLTIAELMLVNEKTWRSSAEIHKGILDIAKVM
                     DDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIEQKGVKSVFEQSDIMNHLNLYAMAVN
                     EENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKKGA
                     SISGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLV
                     QIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGG
                     LAVNLPEC"
     gene            complement(2327..>2854)
                     /locus_tag="ACF2SP_003029"
     CDS             complement(2327..>2854)
                     /locus_tag="ACF2SP_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFD2360787.1"
                     /translation="LYQTVNNLLITMFIAGLWHGASWNYAIWGTYHGCLLAVYHVFRF
                     YYIKRNLQTENQLMKIGSIIMTYSLVVIGWVIFRSTSFEQAFTILGKMYDIPGFVQEF
                     AMNQRASAYTDSLFYLVGMFALCFIGPTFKSIETKILSAVQPFWIKKWLYSLSIFTVL
                     IFMFSPVKPFIYFVF"
BASE COUNT          915 a          593 c          548 g          798 t
ORIGIN      
        1 gggccaatag gtccgccaac gctacgtggg ccatatgcaa gacctgaggg aacataaatt
       61 tcccaagttg atccagctgg catcaattgc aaagcttctg tccatccagg gataacttgt
      121 gaaacctgga acgttgctgg cttaccagtt ttttcggtac tgtcaaaaac ggtaccatca
      181 atcagacgac cagtatattc gacagtgact gtatccgatt ttccgggttt aacaccattt
      241 ccagaattga ttactttgta ttgcaaacca cttggcaata caacaacgcc tggcttgttt
      301 ttgttttcag ttaaaaaggc ttcccctttt actttatttt catccgcttt cttattgaat
      361 tcagcagtac gcttagccat caaatctttc tgaaacttgt taagaacgtc tttcatttgc
      421 tgttcggtta aagccaattg agcgccactc atagcgtctt gcatgccttt agccattgct
      481 tccggattaa catctatgcc ttgattttta aaattcttcc ccaaatcggc accaatgcta
      541 taagacaact tatccttgtc tgtagctaat gatgtggcat cggttgcagc cattgctgtt
      601 gacattgcaa gccccataac agctgcagtc accaatttca tcttcataaa caatcccctt
      661 ttagtcttac acttaagttc tcatactgca caataaattg agcaaaataa agcaaaaaca
      721 aatcgcccac taattctgcc taaatttgcg taaaattacc agtcataagc tatcatttaa
      781 ttatataatg gacaaaaagg taatatcctt ctacacttgc ccctaataat gaacaagaga
      841 ttttaacggc gatgaacatt agtgtttttg atttattttc tataggaatt gggccatcaa
      901 gctcacacac agtaggccct atgttagctg ccaatgcttt tttgcaatta ctggaacaaa
      961 aaaatctgtt tgataaaact cagagagtca aagtagagct ttatggttcg ctggcgctta
     1021 ctggcaaggg gcacggtact gataaagcca ttttaaatgg tcttgaaaac aaagcgcccg
     1081 agacagttga tcctgcctcc atgatcccaa gaatgcatga aattcttgat tctaatctac
     1141 tcaatttggc cggcaagaaa gaaattcctt ttcatgaagc gaccgatttt ttatttttac
     1201 aaaaagaatt attacccaaa cacagtaatg gaatgcgctt ttctgctttt gatggaaacg
     1261 ctaatctact gattgagcaa gtctattact ctattggcgg tggttttatt acaaccgaag
     1321 aagactttga taaatcaaca accgatacca atcccccccc ttaccccttt gcaacagcaa
     1381 ctgagttatt gaaattatgc aaaaaacatc acttgaccat cgctgaattg atgttagtca
     1441 atgaaaaaac atggagaagt tcagcagaaa ttcataaagg aattcttgat attgccaaag
     1501 taatggatga ctgcatcaac aacggttgca aacatgatgg agtgctcccc ggtggcctta
     1561 atttaaagcg acgggcacct gacttgtata gaaaactaat agaacaaaaa ggggttaaaa
     1621 gtgtttttga acagtcagat attatgaatc atttgaacct ttatgctatg gctgtcaatg
     1681 aagaaaacgc agcgggagga cgaattgtca cagccccaac aaatggagct gccggaatta
     1741 ttccagctgt tcttaaatat tgtcagcaag ctcacgacag gatgagcaac gaagacatct
     1801 acacttattt tctgactgcc gcagcgattg gaatccttta taaaaaaggc gcttcaattt
     1861 ctggcgctga agtaggatgc caaggtgagg ttggtgttgc ctcttccatg gcagctgctg
     1921 gtttgacagc ggttcttggc ggaaccatcg agcaggttga aaatgctgca gaaattgcaa
     1981 tggaacatca tctgggaatg acctgtgatc ccgttctggg ccttgtgcaa atcccatgca
     2041 tcgagagaaa tgccatgggg gctgtgaaag cagttaatgc aacccgaatg gcactcatag
     2101 gtgatggtca gcatcaaatc tctttggata aagtcatcaa aacaatgaag caaaccggca
     2161 tggatatgca aagtatatac aaggaaacgt ccatgggtgg attggctgtt aacttgcctg
     2221 agtgttagtt atcaccgctc cctcacggtc gcggctcgga tgattaaatc aaatgataat
     2281 taaacctgag ccgcgaccgt gagggagcgg ataacaaatt atctaattaa aaaacaaaat
     2341 aaataaacgg tttcacaggg gaaaacataa aaattaacac cgtaaaaatt gaaagcgaat
     2401 aaagccattt tttaatccag aatggctgta cagctgacaa gattttcgtt tcaattgatt
     2461 taaaagttgg ccctataaaa cacagggcga acattcccac gagataaaat aatgaatcgg
     2521 tataagctga agcacgttga ttcattgcaa attcctgcac gaaaccagga atatcataca
     2581 tcttaccgag aatagtaaat gcctgttcaa atgaagtaga tcgaaatatt acccagccta
     2641 tgactactaa tgaataagtc attataattg agccaatctt catcaattga ttttcagttt
     2701 gtaaatttct tttaatgtaa taaaaccgga aaacatgata aacggctaac aaacaaccat
     2761 ggtatgtgcc ccaaatcgca taattccagg aagcgccatg ccataaacca gcaataaaca
     2821 tagtaattag taaattattc accgtttgat acaa
//
LOCUS       DAXLDT010000263         2853 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXLDT010000263 DAXLDT010000000
VERSION     DAXLDT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.264"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(20..1216)
                     /locus_tag="ACF2SP_003030"
                     /pseudo
     CDS             complement(20..1216)
                     /locus_tag="ACF2SP_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn1 family transposase"
     gene            complement(1464..2741)
                     /gene="legC7"
                     /locus_tag="ACF2SP_003031"
     CDS             complement(1464..2741)
                     /gene="legC7"
                     /locus_tag="ACF2SP_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC7/YlfA"
                     /protein_id="HFD2360788.1"
                     /translation="MATNETELQVLIQHDSKSTITTSSLDSTDKDPKASTGTPEIESG
                     SLAQIVDTQKQLSQVKESLESIVDSIAENPSLITRAASAWGELPMWQKVTGGLVLTAP
                     TLAVGLFAHIGVLLVIGGVTGLTYTAGAIVLDDHHTCNVNIAKRLKEGLFGLADLLQI
                     TIEALDAIRKKFAEEIEKFKNENLRLTDNIDRLGNEMESLSAQVELYMEIEKMLRKDT
                     NEMEQTVKKLQESTTKQTDLLEKNQKELSKIRKEYEKSQLQLAEKIAELHEVRLSLGL
                     EVQKAKTVAKTLEGTVQTLTGTVIADEEQRVSFQKKLDGFLNDKQLSFDQVAERICKA
                     EEELKKVKEELRQSNDRYSELLKRQEQQVERLEKLGLHKLERIVDKENVKPSNDPVHS
                     GLLSHGIYSTPKGKVTQPKVEVVEDRQTIALVN"
BASE COUNT          802 a          593 c          444 g         1014 t
ORIGIN      
        1 gatcctgtca ttattttact cagctcaaag cgccacattc aataaatgag tgatattgct
       61 gtttcaaatc actcattatg tattgaaata aatcccaatt cagtaattcc ggctcatgtc
      121 gataagccat tctagcaaga tgaataaaag agcgaactcg tttgtttttt atagtatttg
      181 cttgataaga gcgatgttta ttaagggatt caatcattaa accgaaccac caggcaatca
      241 taattaataa aacagcaaga aaaaaaagca tattaactct ggtttttcct gaagattgat
      301 ttcgtcgaaa tcccattccc agttgttcat tttttatgtc tttgaaattt tgttcgattt
      361 gcattctttt tttgtaataa gacacgagaa atcaagcatg cttatttaat gagctaaaga
      421 gaacccaccc atttttataa taattggaat gagctttatc atgagaagga tatttatttt
      481 tcttttttcg taccttgcct gagagttttt ctttataggt ataaagataa ccttccaccg
      541 ccttttttgt ttttccaagc ttgaccttac ctaatgcaga agcttttcca ataccttcaa
      601 aaagaatatc tttcactttg tgccaatttg tttctccttc aatctgatag caatacaaag
      661 aatagatgcg gccaataaca tcccagccta gctcaagcac ctgttgaaac cactcacgat
      721 gaaaacccgc gtctgtaata attgtcacgg acaaactcga aggaataacc tctttcaagc
      781 gtaataaaaa ttgacgatga atctctgcat tttcttgctc tgactcagaa tgaacttcgt
      841 gatagattgg tattgagcga ccatgagctg caatactagc ttccagaaca tgatagccct
      901 cgctacatcc tccagtccaa tcaattaaaa caacaatctc ttttgcatgg ctccaaaaaa
      961 aatgggtcag actcttataa atgcaaacaa tatccctttc cagtttgaaa ttattcacga
     1021 atgtttgagc cgcatatatt ttacttttaa ctgtcgtttt ggatgttaat tttttaccaa
     1081 tctctgtaac ggataatgtg gtatcgtagt ccattaagtc acttaaaaag cgaaccaaac
     1141 attgttttct tttagcatga attgaattct ttaatgcgct atccaataaa ttctggcaaa
     1201 atattttctt gtgcatacaa cttccgattg gttttgtctt ggtaaacaaa tttgatcatg
     1261 aatctgtatg cacttcaatt tcttttccca tctttgttaa tgtacacttt aaaaaagtaa
     1321 ctgctcaatt aaatgacagg attcctcagc gccttcgcgg gaaagacaga ggacttaata
     1381 ttctttgatt gcatatccct taaccggtta cttaaaatta taacctttaa aacagcctga
     1441 ttcttcttcc ttaaatcctt ctcttaattg actaaagcaa tagtttgtct atcttctact
     1501 acctctactt taggctgggt gactttaccc ttgggcgtgc tatagattcc atgagaaagc
     1561 aaacctgagt ggacagggtc attactgggt ttcacattct ctttgtctac tattctttcc
     1621 aatttatgca agcccaattt ttccaaacgt tcaacctgtt gttcctgacg ctttaacagt
     1681 tcgctataac gatcattgct ttgccttaat tcttctttga cttttttaag ttcttcttct
     1741 gccttgcaaa ttcgttcagc tacttgatca aagctgagct gtttgtcatt caggaatcca
     1801 tccagtttct tttgaaaaga aacacgttgc tcttcatcag caataaccgt tccagttaaa
     1861 gtctggactg taccttccaa cgttttcgca actgttttag ctttttgaac ttcgagaccc
     1921 aacgacagtc tgacttcatg taattcagct attttttcgg ctaactgcaa ttggcttttt
     1981 tcgtattcct ttctaatttt tgacaattcc ttttgatttt tttccaataa atcagtttgt
     2041 tttgttgtag attcttgaag ttttttgacg gtttgctcca tttcatttgt atctttacgg
     2101 agcattttct caatttccat atataactca acctgagcac tcagtgattc catttcattg
     2161 cccagcctat caatgttatc agttaatctg aggttttcat tcttaaattt ctcaatttct
     2221 tcagcaaatt ttttgcgaat tgcatccaat gcctcaatcg taatttgtaa cagatctgcc
     2281 aaaccaaaaa gtccttcttt taatcgtttg gcaatattca cattgcatgt atggtggtcg
     2341 tccaaaacaa ttgcacctgc ggtataggtt agtcctgtaa ctccacctat tactaataag
     2401 acaccaatgt gcgcaaataa tcctactgcc aaagtaggcg cagtcaggac aaggccaccc
     2461 gtgactttct gccacatagg cagttcgccc caagctgacg cagcgcgagt aatcaggctg
     2521 ggattttccg ctatactgtc tactattgat tccaggcttt ctttaacttg gctcagttgt
     2581 ttttgggtat cgacgatttg ggcaagacta ccgctttcaa tttcaggagt gccagtactt
     2641 gccttaggat ccttatcagt actgtccaag ctagaagtag tgattgttga tttgctatca
     2701 tgttggatca atacttgaag ctctgtttca ttagtagcca tatttactcc ttacgattaa
     2761 aaatcttcct tagatgtcca tttttaatca taaattatca ataagacaag taattcttgc
     2821 attttttgca attaaatttt attttaataa acc
//
LOCUS       DAXLDT010000264         2847 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXLDT010000264 DAXLDT010000000
VERSION     DAXLDT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.265"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            51..596
                     /locus_tag="ACF2SP_003032"
     CDS             51..596
                     /locus_tag="ACF2SP_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFD2360789.1"
                     /translation="MKLLFDFFPIVLFFIVYKFFGIYTATAVAMVASLTQVAFYRLKF
                     QHYEKMHLFSLAIIMVLGGATLFFQNPWFIKWKPTGIYWLSALVFYGSGYIGSKPLIQ
                     KMMETNINLTTKIWYRLNLAWTLFFIVMGALNLYVAYHYDTDVWVNFKLFGGVGFTLL
                     FVLIQAFYLTKHTDEKSFEKQ"
     gene            657..1334
                     /locus_tag="ACF2SP_003033"
     CDS             657..1334
                     /locus_tag="ACF2SP_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2360790.1"
                     /translation="MRLLLVEDDELLGDAVKAGLTQFGYVVDWLKDGEAARAALKSES
                     FELIILDLGLPKLSGLSLLQNIRHDGNSTPVIILTARESIEDRVKGLDSGADDYLIKP
                     FDLNELSARIRALVRRSQGRADSVLQYKNITLDPAAHSVFVDDVLVNVPRREFALLQK
                     LLENSGQVLSREQLMQSIYGWEEDVDSNALEVHIHNLRKKLNANFIRTIRGVGYMAEK
                     NDSVLLS"
     gene            1331..2746
                     /locus_tag="ACF2SP_003034"
     CDS             1331..2746
                     /locus_tag="ACF2SP_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2360791.1"
                     /translation="MKSSIRKFLLINLLLAITITTTLTAIGNYYLDQKDIQDHLDTLM
                     AISALSYQALLGDDLHQRPLAKIQNALESIPQKINTYYQKRYLNDEPQENYLDKFNFQ
                     VWTNGGKLLLHSPTAPKIPLTAETDGFSDKRISNQDWRVFTTYNDKAGIRTVLAERYD
                     TRNELGHRIAQDDLYIMLLTFPLSGLLIWIIIGRGLDSLDRVAEEVANRAPSHLEPVD
                     LQEVPEEIKPVIDELNKLFFRLKEGFEREKRFAADAAHELRTPLAALKAQAQVALNSN
                     DIEEKNQALQKLIASVNRSTHIVQQLLTMSKLVPEATELHEQDEVNLSKLTREILAML
                     APSAVEKQIELEFETDEKMPKIYGNPTALGILIRNLVDNSIRYSTENGRVIVRLLKHN
                     NEIILEVCDNGPGIPPELQKRVFERFFRVLGNKSPGSGLGLAIVQQICELHGGRVVLD
                     SPKIGTGLIVRVYLPIKTKPK"
BASE COUNT          953 a          484 c          534 g          876 t
ORIGIN      
        1 ctaaaacttg tgttataatg atattaatta attgataaat taaacaatat atgaaattac
       61 tattcgattt ttttcctatc gttttatttt ttatagtcta taaatttttt ggcatttata
      121 ccgctactgc tgttgctatg gtagcttcat taactcaagt tgcgttttac agattaaagt
      181 ttcagcatta tgaaaaaatg catttgttta gtctggctat cattatggtc ttgggaggag
      241 cgacgttatt ctttcaaaac ccttggttta tcaaatggaa gcctacagga atctactggt
      301 tatctgcact tgtcttttat ggttcaggtt atatagggtc taagccacta atccaaaaaa
      361 tgatggaaac aaacatcaat ttaacaacaa aaatttggta tcgacttaat ttggcatgga
      421 cattattttt tatagtaatg ggggcattaa atttatatgt tgcctatcat tatgacactg
      481 atgtatgggt taattttaaa ctatttggtg gtgttgggtt tactttactt tttgtgttga
      541 ttcaagcttt ttatttaaca aaacacacag atgaaaaaag ctttgaaaaa caatagtgac
      601 atctctgtat ctgcagaaaa atatagcatg taataagaga aaaagtagga gcaccgatga
      661 gattattact tgttgaagac gatgaactgc ttggcgatgc agtaaaagcc ggactgacac
      721 aatttggtta cgttgttgac tggctaaaag atggcgaagc tgctcgggcg gctctcaaat
      781 cagagtcttt tgaattaatt attcttgact tgggattacc taaattatcc ggtttatcat
      841 tattgcaaaa catcagacat gatggaaata gcacacctgt gattattctt acagctcgag
      901 aatcaataga agatcgggta aaaggcttag atagcggtgc tgacgattat cttattaaac
      961 cttttgattt aaacgaatta agtgcaagga ttagagcatt agtaaggcgc tctcagggca
     1021 gagctgattc cgtgctgcaa tataaaaata ttactctcga tcctgctgct cattctgtat
     1081 tcgtggatga tgtactcgtc aatgttccaa gaagagaatt tgctctatta cagaaattac
     1141 ttgaaaacag tggccaggtt ttatctcgag aacaattaat gcagagtatt tatggctggg
     1201 aagaagacgt ggacagtaat gctctagaag ttcacatcca taatttacgt aaaaaactta
     1261 acgcaaactt tatccgcact atacgtggtg taggatatat ggctgaaaaa aatgacagtg
     1321 tacttctatc gtgaaatcgt ccattcgaaa atttttatta attaatctgc ttcttgcaat
     1381 tactattact acaacattaa ctgctattgg taactattat cttgatcaaa aagacattca
     1441 agaccacctt gatacattga tggctatttc cgcgctatcc tatcaagcct tattagggga
     1501 tgatcttcat caaaggcctc ttgcaaaaat tcaaaatgcc ctggaatcta ttccccaaaa
     1561 aattaatact tattaccaaa aaagatattt aaatgatgag cctcaagaaa attatttgga
     1621 taaatttaat tttcaggtat ggaccaatgg aggtaaatta ttattacatt cacctactgc
     1681 ccctaaaata ccattaacag cagagacaga cggctttagc gacaaaagaa tttctaacca
     1741 ggattggcga gtattcacaa cttataatga caaagcagga attcgtactg tactggcaga
     1801 gcgttatgat accagaaatg agctgggtca cagaattgcc caagatgatc tctatattat
     1861 gttgcttact tttccattat caggactatt gatatggatc attattggaa gaggactaga
     1921 cagtctggat agagtagcag aagaggttgc taaccgtgca ccaagtcacc tggaacctgt
     1981 tgatttacaa gaagttcccg aagaaattaa acctgtaatt gatgaattaa ataaattatt
     2041 ttttcgactc aaagaagggt ttgagcgtga aaaacgattt gctgccgatg cagcacatga
     2101 gttgagaaca cctcttgcag cacttaaggc ccaagctcaa gtggctttga actccaatga
     2161 tatagaagaa aaaaatcagg ctttacaaaa attaattgcc agcgttaatc gaagcacaca
     2221 tatagtgcag cagttgctaa ccatgagtaa acttgtacca gaagcaacag aactacatga
     2281 gcaagatgaa gtcaatctaa gtaaattaac tcgtgaaata ttagccatgt tggctccgtc
     2341 tgcggtcgaa aaacaaattg agctggaatt tgaaaccgat gaaaaaatgc ctaaaattta
     2401 tggaaatcca acggctttgg gaatattaat acgcaatctg gttgacaatt ctattcgata
     2461 ctccacggaa aacggaagag ttattgttcg attattaaaa cataataacg aaatcatcct
     2521 cgaagtatgt gataatggcc cagggatccc acctgaatta caaaaacgtg tttttgaaag
     2581 attttttaga gtattaggaa acaaaagccc tggtagcggt ctgggtttgg ctatcgtgca
     2641 gcaaatatgc gaacttcacg gtggacgggt cgtgcttgat tcgcccaaaa ttggcacagg
     2701 cctaattgtg agagtttatt tgcccatcaa aacaaaacca aaataaactc tataatcaaa
     2761 tcataactct tatcatttaa taataactac accacctttt ttcaattaat accgtaaaca
     2821 actttttgcg ataagtcaga ttgataa
//
LOCUS       DAXLDT010000265         2843 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXLDT010000265 DAXLDT010000000
VERSION     DAXLDT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.266"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            1..407
                     /gene="rlmJ"
                     /locus_tag="ACF2SP_003035"
                     /pseudo
     CDS             1..407
                     /gene="rlmJ"
                     /locus_tag="ACF2SP_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213608.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
     gene            411..1175
                     /gene="gloB"
                     /locus_tag="ACF2SP_003036"
     CDS             411..1175
                     /gene="gloB"
                     /locus_tag="ACF2SP_003036"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFD2360792.1"
                     /translation="MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAGPIIRFAQSNQ
                     LTLRTILLTHHHYDHIGGVDLLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLH
                     FHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
                     NTKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFL
                     RTDKEQVKQYALSHGANSSDSLDVFKVLRNQKNSFK"
     gene            1289..2728
                     /locus_tag="ACF2SP_003037"
     CDS             1289..2728
                     /locus_tag="ACF2SP_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD2360793.1"
                     /translation="MNCNLLIIKRFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLS
                     HEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESA
                     YDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
                     GNWILAIAAYDSGEGTISRALKSTARNGRKVQFWNLPVPRETKVYIPRLLALAEIINN
                     PKRYKIALPNIPYLPYFEEVNIGSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYK
                     PFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLN
                     QLTSNKVQLNQSLLIPSSKNSPVVAKIPPQEKKPATKQLAPMKNVRIIHIVQNNDSYQ
                     KIQKAYGVAAKEIQNWNKLANNAPLRKGQRLVIWKRVKQPVEYIVKSGDSLSVIAKKH
                     QTKVNTIISLNPGLKKSMPLRLGQKILIG"
BASE COUNT          974 a          552 c          491 g          826 t
ORIGIN      
        1 aaattaaatg cccttttgcc gccaccagaa aaacgcgggt taatttttat tgatccctca
       61 tatgaaagaa agaagaatac aaagaaattc catacgctat aaaaaatgct tactcaaaat
      121 tttctaccgg cttatattgt gtttggtatc ctgttgtcaa taaagcatgg actgaacaat
      181 tcttgaggaa aatgagagaa attagttcca aatcggtaag gattgagctt cacttgaatc
      241 cactcattaa tgaaggaatg actggttgtg gtctttggat aattaaccct ccgtatactt
      301 tcccttcaga aatcaagata gttttagaga cattaacaac ttattttaac cctggctcat
      361 cttcttatat cattgaatca ggttcaaaat tatgtcacga gttataaatt atgactatcc
      421 tgcctatttc ggctttttca gataattata tctggacctt tattgataaa atagctggcg
      481 tattagattg tgtcgatccg ggagaagcag gaccaataat acgctttgcc caatccaacc
      541 agctgacact aagaacgata ttattaaccc accaccacta tgatcatata ggaggtgttg
      601 acttgctaat caagcaatgg ccatcttgta aggtctatgg ccccatagat gagcgaatca
      661 acaatgtaac ccacccaatt aaacaaggtc aatctgttca agtgggctct cttcactttc
      721 atattttatt taatcctggg catacttcta cccatatcag ttattatgaa ccacaacaag
      781 gatggttatt ttgtggagat actctatttt ctgctggttg cggtagagtg tttgatggaa
      841 ctatagagga attgcatgaa tccctgctct tatttaaaaa actcccgcga aatactaaag
      901 ttttttgtgc tcatgaatac accttacaga atttaaaatt tgcacatacc gttgagccct
      961 gcaattcatc agtcatcaat tacatgcaac aaattctgaa acaaccatca ccatgtactc
     1021 tcccgtctaa tatagacttg gaattatcaa ttaatccttt tttacgcact gataaagagc
     1081 aagtaaaaca atatgctctc tcgcatggtg cgaattcttc agattctctg gacgttttta
     1141 aggtattgag aaatcaaaaa aactcgttca aataatacaa caaaggatca aataaaaacc
     1201 catttgcgcc caattaaacc agaaagggtt taatccttca ttaattttta gtaaactgat
     1261 tagccaatta taataacagg taagaccatt gaattgtaat ttattgatta taaaaagatt
     1321 tttttcattc atccttattt ttattggtat ttctaacccg gcaatttctg gcacagctcc
     1381 agatgtatgg gatgtattac gtagtgaatt taccttaagc catgaagtaa ctcgcccaga
     1441 agtgcaagaa caaatcagat ggctaatttc tcatcctagt tatatcaaca aagtaagtag
     1501 ccaatctgaa ccttacattt accacgtgtt aggtgagata aagaaaagaa aactgcccgg
     1561 agaattggcg ctactcccca tgatagaaag tgcatatgat ccttttgcct attcaggagc
     1621 aggcgctgct ggtatatggc aactcatgcc tcgaactgga agcgatcttg gattaaagca
     1681 agactggtgg tttgatggaa gacgcagcat tggcccttca actgatgccg cattaaatta
     1741 cctagtctat ctaaataaat attttagtgg taactggatt ttagctattg ctgcttatga
     1801 ctctggagaa ggaacaattt cgcgggcgct caaatcaacc gcacgtaacg gacgtaaggt
     1861 gcaattctgg aatctgccag ttcccagaga aaccaaggta tatatcccgc gtttattagc
     1921 tctggcagaa attattaaca atccaaaacg gtataaaata gcacttccca atattcccta
     1981 tcttccttat tttgaagaag tcaatattgg tagtcaaatt gatttaaatc atgccgctaa
     2041 actcgccgga atcagctata aggagttgat taaactaaat cccggttata atcgatggac
     2101 aactgcacct tataagccat ttaaattact aatacctgct gaaaaagtcg aaagatttaa
     2161 cttgaatcta tcgaatatac ccgaagataa gcgcgtaaac tggactaaac atcaagtaaa
     2221 aagaggtgac agcttggatg ctattgcaaa gagatatcac acaacagtca atttgataaa
     2281 acaactcaat caattaacca gcaataaagt tcaacttaat caatccctac tcattcctag
     2341 cagcaaaaat tccccagtag tagctaaaat acctccacag gaaaaaaaac ctgctactaa
     2401 acagctggca cctatgaaaa atgtccgcat tattcatatc gtacaaaata atgatagcta
     2461 tcagaaaatt caaaaagctt atggggttgc tgctaaagag atacaaaact ggaataaatt
     2521 ggcaaataac gctccattac gtaaaggcca gcgactggtc atatggaaaa gagtcaagca
     2581 acctgttgag tatatagtaa aatcagggga tagtttgagc gttattgcca aaaagcacca
     2641 aacaaaagtg aatactatta tcagtttaaa tccaggactt aaaaaatcaa tgccccttcg
     2701 cttgggacaa aaaatattaa ttggctagat tatatgcatt atcgtgatat aaactagata
     2761 gattaatgca tctgtttgat acagaaacgt gcttttgagg aataccaaaa ctactggcat
     2821 gccgctaaaa acttaaagat tat
//
LOCUS       DAXLDT010000266         2777 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXLDT010000266 DAXLDT010000000
VERSION     DAXLDT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.267"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1863)
                     /gene="ravR"
                     /locus_tag="ACF2SP_003038"
     CDS             complement(<1..1863)
                     /gene="ravR"
                     /locus_tag="ACF2SP_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavR"
                     /protein_id="HFD2360794.1"
                     /translation="MLSQGVRRMGKEKRELEASYIPLPRKGRFWIHSESCYTEEELNF
                     REWILKIRSNSGIHPDYLNNPSSWFASDDQALVNLYEHAWFYSNAYKPRHVLSLDDFH
                     VQPIDNQRLQWPQLYDLMQKVGDYLLLRNKELSPEHLNQPMNFVLLDVNNILKGLSQN
                     NNIQQVKDQLESVTRYLRTIEKNISPLIGSDRLFLANFRKTVDTKIHPHLDHMLETQL
                     LRDKLGELSKDIRKLSSERNRILHYALNANPVNPHSYEFELDKISDSASYPTQAAKHC
                     AEKSNELVSDPLPILHLTKEQLSDCPHFKLITMKDEILDHYAKAVSDLNELERFQKVI
                     QEIMDLLGQAGEVYTVHQFKNQMQSLLTQIDSFIDASSVHIDEIIDANTQAYHKAIQE
                     EQNLPFWKNWLTNEKEKLKIFIKNQDTLSQFPSSSSDLKKTNKALKNRVNEVVSHLNQ
                     PKLKETSFEAIAGQTQALNKLMLSMHDWMKIQHELKGLPLPQPPEPLKIMPTPIPTVD
                     VFSSPPPPLHAYKPLFTALPGNTTAPCTPDNNQCKTTPSSPTQNSLGYLGLIALIPIG
                     VIALYLVIHWMNKKDAQIRVSGTEEEFNKIKEKFEDLLAELKGFEHSEEIDLSDD"
     gene            2123..2608
                     /locus_tag="ACF2SP_003039"
     CDS             2123..2608
                     /locus_tag="ACF2SP_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFD2360795.1"
                     /translation="MPSFDIVSKMNEVDLRNAVDNAVREVSTRFDFRGVEATIELKDL
                     TVTLRSESDFQVRQLEDLFRNHCSKRNLSTSGVDIEDEPVHSGKFYTLTMTFKQGIDQ
                     PTAKEIVKYIKETKAKVQTSIQGDKVRVTGKKRDDLQETIALLKKSNIELPLQYENFR
                     D"
BASE COUNT          824 a          452 c          562 g          939 t
ORIGIN      
        1 gtcatctgag agatcaatct cttccgaatg ctcgaaacct ttaagttcgg ctaatagatc
       61 ttcaaatttt tcttttatct tattgaactc ttcttctgtg ccagatactc gtatttgagc
      121 gtctttttta ttcatccaat gaataaccaa atataaggct atgacaccaa taggtatcaa
      181 ggcaattaat cctaaataac ctaaagaatt ttgtgtgggt gaactggggg ttgttttgca
      241 ttgattattg tcaggcgtac aaggagctgt tgtattgcca ggcagggcgg taaatagagg
      301 cttatatgca tggagtggag gcgggggaga agagaacaca tcaactgttg gtataggagt
      361 tggcattatc ttgaggggtt caggtggttg gggaagtgga agccctttca attcatgttg
      421 gattttcatc cagtcatgca ttgataacat caatttatta agtgcttgag tttgtccagc
      481 tattgcttca aaggacgttt cttttaattt aggctgattt aaatggctaa cgacttcatt
      541 gacccggttt tttaaagcct tgtttgtttt tttgagatca gaagaactgg atggaaattg
      601 agagagtgtg tcctgatttt taataaaaat ctttagtttc tctttttcat tagttaacca
      661 atttttccag aagggtaaat tttgttcctc ttgtattgcc ttatgatagg cttgagtgtt
      721 cgcatcaatt atttcatcaa tatgcactga ggaggcgtca ataaagctgt caatttgggt
      781 cagtagggat tgcatttgat ttttgaattg atgaacagta tagacttcgc cagcctgccc
      841 cagaagatcc atgatttcct gaatgacttt ctgaaatcgt tccaactcgt taagatcaga
      901 gactgctttg gcataatgat caaggatctc atctttcatc gtgattaatt tgaagtgagg
      961 acaatcactt aattgctctt tagttaaatg cagtataggc agaggatctg agactaattc
     1021 atttgatttc tcagcgcagt gtttggctgc ttgggttgga taagaagcgg agtcactgat
     1081 tttatccagt tcaaattcat aagagtgggg atttaccgga ttggcattaa gagcataatg
     1141 caagattcta tttcgctcac tggataattt tctgatgtct tttgataact ctcccaattt
     1201 atctctaagt aattgagttt ccagcatatg gtccagatga gggtgaattt tagtatccac
     1261 tgttttgcga aagttggcta aaaataatcg gtctgaacca atcagtggtg aaatattttt
     1321 ttcaatagta cgcaggtagc gagtgactga ctcaagctga tcttttactt gttgtatgtt
     1381 gttgttctga cttagccctt ttaagatatt gttcacatca agaaggacaa aattcatggg
     1441 ttggtttaaa tgttcggggg ataactcctt atttcttaat aaaagatagt cacccacttt
     1501 ttgcattaag tcataaagtt gtggccattg gaggcgctga ttatcaatgg gttggacatg
     1561 gaaatcatca aggcttaaca catgtctcgg cttgtaggca tttgaataaa accaggcatg
     1621 ttcataaaga ttaaccaaag cttgatcgtc gctggcaaac caggagctgg gattattaag
     1681 ataatccgga tgaatccctg aattggaacg aatttttaaa atccattctc tgaaatttaa
     1741 ctcttcctct gtgtagcaac tttcagaatg aatccaaaag cgtccttttc taggtaacgg
     1801 tatatatgaa gcttcaagct ctctcttttc ctttcccatc cttctcactc cttgagaaag
     1861 caaaaaaaaa cctaaggtaa ttccagatta acactagatt agcaatagct ttttttgctg
     1921 ccgattaata atatccataa ccaactgggt aatttttatt ccaatttatt tgataacatt
     1981 ctttgtgcaa ttatgcaacg gttaatcaat tgatttattt ttaaacaact ttgatttttg
     2041 caagaaagga aatagaggtg ttgtttcttt cacctatccc tgttttttga taaattacaa
     2101 ttttaattta aatggattgg ttatgccttc ttttgatatt gtttctaaaa tgaatgaagt
     2161 ggatttgcgc aatgcagtag ataatgcagt gcgggaagta agtacccgat ttgattttcg
     2221 aggagttgag gctactattg agcttaaaga cctgacggtt actttgcgtt ctgagtctga
     2281 ttttcaagtg aggcagcttg aggatttatt ccggaatcat tgctcaaaac gaaacctcag
     2341 cacatcagga gtggatatag aagatgaacc tgtgcatagt ggaaaattct atactcttac
     2401 catgactttt aaacaaggca ttgaccaacc tactgctaaa gagatagtga agtatatcaa
     2461 agaaaccaag gccaaggtcc aaacttctat tcagggggat aaagtgaggg tgactgggaa
     2521 aaagcgagat gatcttcaag aaaccattgc tttattaaaa aaatcaaata ttgaattacc
     2581 tttgcagtat gaaaattttc gtgattaatt aatatgaatt cgatataaaa ggcaaagaaa
     2641 agccttttat atccttaaat gaacgatatc acgaaatgca tagcatctat acgacattca
     2701 cattgaaaga tccagtatat ggttctaatt aaaatctgtt attgttaatt aatgaaatta
     2761 cttttgcgtt agacttg
//
LOCUS       DAXLDT010000267         2756 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXLDT010000267 DAXLDT010000000
VERSION     DAXLDT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.268"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(95..574)
                     /locus_tag="ACF2SP_003040"
     CDS             complement(95..574)
                     /locus_tag="ACF2SP_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360796.1"
                     /translation="MPSEKSRYLNRGPKSPVDMHQLKKYLNSFTKEHLAEIVLLNAQY
                     NSVLWRALSASIGMRLANGDWEEIKKAIDYAFYFPEYIRYTENGYGFIIYEMINALEF
                     LYKDRDKQFILQVADYMFEQAEQALESFEEGWDWTCALESLKDWIRNKKSNVNEGVT"
     gene            1405..2520
                     /locus_tag="ACF2SP_003041"
     CDS             1405..2520
                     /locus_tag="ACF2SP_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360797.1"
                     /translation="MKKHNNWHYARPELAKKYLDLFEIGLTSARGLFARRRMGKTEFL
                     KQDFIPAAQKAGYIVVYSNLWELEIDPATALVSEFYKTIEPKGFNKIWTSLNKPINIK
                     KVKASGKITGLGERTLETDLAETKKGTSTLLMEAMNAFDKTKDQMILVIDEAQVLAYE
                     ENSHFAHALRAALDIRKERIKVIFAGSSETTLRRMFGVASEPFYNWAPLEPFELLGKD
                     FVLAMVERVNKISKYPMSIHDALDAFEKLKKTPEFFRRYIEYYLSNPEEGTAAALAAT
                     KSRVFSDDNFKRQWETLLPADKIIMSMIANGISDLHGKLALQKLGEALGIDSVNKNTT
                     HNALRRLSNKNLITKIDFGTYQFEDEAFADWVKHLAD"
     gene            complement(2538..>2756)
                     /locus_tag="ACF2SP_003042"
     CDS             complement(2538..>2756)
                     /locus_tag="ACF2SP_003042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360798.1"
                     /translation="SKFLPQSIEEASKITTDPFKIGDRVYFVNDQTNETWTIVNFSLS
                     SIDKKPVAILRSDTFDTYSSLGNIKLIC"
BASE COUNT          955 a          464 c          468 g          869 t
ORIGIN      
        1 caacatctct ttcacgattg tgcgcctgct tgcgatccct ttcatttagg agaaagccaa
       61 tgagataaaa gttgtttttt ctgtgttaat tctattaagt tacaccttca tttacatttg
      121 attttttatt tcttatccag tctttaaggc tttctaatgc acaagtccaa tcccaacctt
      181 cttcaaaaga ttcaagagcc tgttctgcct gctcaaacat ataatcagca acctgcaaaa
      241 taaattgctt gtctctatct ttatacaaga actcaagcgc attaatcatt tcataaataa
      301 tgaatccata cccattttct gtataacgga tgtactcagg gaaataaaat gcatagtcaa
      361 tggctttctt tatctcttcc cagtctccat tggcaagtcg cattccaata gatgcactta
      421 aagccctcca taagacagaa ttatattgtg catttaaaag aacaatttca gccaaatgtt
      481 ctttagtaaa agagtttaaa tactttttca gctgatgcat atccacaggt gatttaggtc
      541 ctctgttaag gtaccgagat ttttcagaag gcattaactt ccccttgatt taaacgttat
      601 tctaatattc acccattgag caccatgcta cttttgctac tcgttgacaa acgagcatac
      661 ctatattgat cagataatac agtgttcaca aggatcataa caatattcat cgcatcaaaa
      721 tgaatgcatt tatgtagcaa tatattgcta cataaatgct acatatcgct acaatttcag
      781 agattaataa atttcactgg atcacgtctt gtttctgcca ttttccttat ttatagaaaa
      841 ttattataaa taaggaactc tttgcaaaaa acaaaagaac ttaaaaaaac aaaacatgaa
      901 gaaacccaag ccacaactga ggacaccctc ttttgcctat ttcaggatgt gttttttcgg
      961 tgttcttagt gtagcgttat gtagcaatac agtagcacga gttttgctac aattaattaa
     1021 tcggatttat aacagaaaaa accgaattac cctatgattt aatggataat tttatccatc
     1081 aggtttcaat gatttgatgg tcgtgtagca atgtagcaaa tacattaaat gacaaattgt
     1141 ttaggacatt ttgcgaataa gagtctatat ttgatcctta aaggatatat cgattataaa
     1201 gaaaatgttt gagattcaat caacccaaaa gtaaacattt tctaaagcaa gaaatagacc
     1261 acgaaggttt atgcacatac taagacactt ctgataattc ttaaaaagtc ttaaatacga
     1321 taagaatttt taagactttc attgttaatg attaggctat cctatagaat aattcttaaa
     1381 tcctttaaga attattaaga acttatgaaa aaacacaaca attggcatta tgcccgcccc
     1441 gaactagcta aaaaatatct cgatctattt gaaataggtc taacctctgc ccgaggatta
     1501 tttgctagac gaagaatggg aaaaaccgaa tttttgaaac aagattttat acctgcagct
     1561 caaaaggcag gttatattgt cgtctatagt aatctttggg aattagaaat tgatcctgca
     1621 acagctttgg tttccgaatt ttataagaca attgagccaa aaggatttaa taaaatttgg
     1681 accagtttaa acaagcctat aaatattaaa aaagttaagg cctcaggaaa aattacaggt
     1741 ctaggggaaa ggactcttga gacagattta gcagaaacaa aaaaaggaac cagtacttta
     1801 ttaatggaag caatgaatgc tttcgataaa acaaaagatc aaatgatttt ggtaatagat
     1861 gaagcccaag ttttggcata tgaagagaac tctcattttg ctcatgctct aagagcagca
     1921 ttggatatca ggaaagagcg tataaaagta atttttgctg gcagttcgga aaccactcta
     1981 agacgtatgt ttggggttgc atcagagcca ttttataact gggcgcctct agaacccttc
     2041 gaattattag gaaaagattt cgtattggct atggtcgaaa gggtaaataa aatatcgaaa
     2101 tacccaatga gtattcatga cgctcttgat gcatttgaaa aactgaagaa gactcctgag
     2161 ttttttcgaa gatacataga atactattta agtaatccgg aggagggcac ggctgcagct
     2221 ttagcggcta ctaaaagcag ggtatttagt gatgataatt ttaagcgtca atgggaaacg
     2281 ttattgccgg ccgataaaat aataatgtca atgattgcca atggaataag tgacttacat
     2341 ggaaaactag ctttgcaaaa actcggtgaa gcccttggaa ttgatagtgt taataaaaat
     2401 accactcata atgcactaag aagattatcg aataaaaatt tgattactaa aattgatttt
     2461 ggaacttatc agttcgaaga tgaggctttt gctgattggg taaagcattt agctgattaa
     2521 ttttgaaatc agcccaatta acaaattaat ttgatatttc ccagggaaga ataggtatca
     2581 aaagtatcgc ttctgagaat agccacgggt tttttatcaa tagagctcaa tgaaaaattc
     2641 acaattgtcc atgtttcatt tgtttgatca tttacaaaat atacacggtc tccaatttta
     2701 aaaggatcgg tggttatttt acttgcctct tcaatagatt ggggtaaaaa tttgct
//
LOCUS       DAXLDT010000268         2746 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXLDT010000268 DAXLDT010000000
VERSION     DAXLDT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.269"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(262..534)
                     /locus_tag="ACF2SP_003043"
     CDS             complement(262..534)
                     /locus_tag="ACF2SP_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360799.1"
                     /translation="MKLRLYHGRNTPEQEMDDWGFEGATLFGVDGIIWTYGVPRVFFI
                     NDEYFNIAREVTGWDEIADGLEMRVYEDLIKTKDGYFGDWELIKLG"
     gene            complement(621..1424)
                     /locus_tag="ACF2SP_003044"
     CDS             complement(621..1424)
                     /locus_tag="ACF2SP_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFD2360800.1"
                     /translation="MLPILSKEKLFKLAPSIFTQESSHKTSAQYSPISTEQIIEKLMS
                     EGFFPTWATQTKSLSEESKAFAKHMLRFQRHDVIQNNQGLYPELVLINSHDGLSSYRL
                     MAGLFRVVCGNGMIAGQSYDEIRIKHQGDVVGNVIEGTYKVIETANKMLDVSDDMASI
                     HLNDTEKMIFAEAAHSLKFSNDEGAMIVAPERLLQPRRYVDQKDNDLFTVFNVLQENL
                     IKGGIRGHRLNKYGYTTRTKTREVKAIDQNVKLNRALWTLTEKMMEIKR"
     gene            1616..1939
                     /locus_tag="ACF2SP_003045"
     CDS             1616..1939
                     /locus_tag="ACF2SP_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360801.1"
                     /translation="MTIPEIIQQQLLRTNKAIVWSWGVSKWYELSSNALAIRVHARYL
                     DGFVCIELDEAQDLYNISFYLNQDFSDILVWPVIPYKPMKGVYCDQLVEFIDNRIERI
                     PDYKY"
     gene            complement(2280..2411)
                     /locus_tag="ACF2SP_003046"
     CDS             complement(2280..2411)
                     /locus_tag="ACF2SP_003046"
                     /inference="COORDINATES: protein motif:HMM:NF023007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFD2360802.1"
                     /translation="MIKQCKRLLFFFIRKACCLYFKLPNSGTYHGIINIQVDVLFKT"
     gene            2448..>2746
                     /locus_tag="ACF2SP_003047"
     CDS             2448..>2746
                     /locus_tag="ACF2SP_003047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360803.1"
                     /translation="MKIQAVFSELLSIHNKSEKLHQKARMLFNKDKSIAPKTIEEIAK
                     GELSTSKWLVACAVEILLEEKPDIVQKILTEDDILFLWANPLLKNHRPLKELLDKL"
BASE COUNT          905 a          520 c          464 g          857 t
ORIGIN      
        1 gaagagtcat caattatgat tttctctaaa gacaaagaat ggaagcaaca tcgaaaagac
       61 aaaaaactga ataacgaaac cgtctaatgt ttatcagata gggacttact tttgaaaaaa
      121 tatcagcaac attttattta cctttccttt ttttaattgc gtataatatt ccttccaata
      181 aaagcagaaa aaaagagctg ataggatagc tgaatcgata agaaaaaaag cccatggaat
      241 ttgggctttt ttatgggaat tctaccctaa tttaatcaac tcccaatcac caaagtaccc
      301 atctttggtt ttaattaaat cttcataaac acgcatctct aaaccatcag caatctcatc
      361 ccaccctgtt acttccctcg ctatattaaa atactcatca ttaataaaaa acactctggg
      421 aactccgtat gtccagataa tgccatcaac accaaaaagt gttgctcctt caaagcccca
      481 atcatccatt tcttgttctg gggtattgcg tccgtggtat agtcttaatt tcatcaaatg
      541 ttcctttaat cttgctgttg ttatcacgct attttcagga tgaggcaaag ggatttttct
      601 ttgcctctta aaaaattccg ttaccttttt atttccatca ttttttcggt taacgtccac
      661 aatgctcgat ttaatttcac attttgatca atggctttta cttctctcgt ttttgttcga
      721 gtggtgtatc cgtatttatt taaacgatgg ccacggatac cccctttaat taagttttct
      781 tgcaggacat taaacaccgt aaacaaatca ttatcttttt gatcaacata acgccttggt
      841 tgcaataaac gctcaggagc aacgatcatt gcaccctcat cattagaaaa ttttagagaa
      901 tgggcagctt cggcaaaaat catcttttca gtatcgttta aatgaattga agccatatca
      961 tctgatacat cgagcatctt gtttgcagtt tcaatcactt tataagtgcc ttcaataaca
     1021 tttccaacaa catccccctg atgtttaatt ctgatttcgt catacgattg cccagcaatc
     1081 attccattac cacacacaac ccgaaaaaga cccgccatta gacgataaga agacaggcca
     1141 tcatgagaat tgataagaac aagttctgga taaagtccct gattattttg aatgacatca
     1201 tggcgttgaa aacgaagcat gtgttttgca aatgctttcg attcttcact caggctttta
     1261 gtctgagttg cccaagttgg aaaaaatcct tctgacatta atttttcaat gatttgctct
     1321 gttgagattg gtgagtactg agcacttgtt ttgtgggaac tttcttgtgt aaatatggaa
     1381 ggggctaatt taaatagttt ttcttttgat aaaatgggta acatgatgtt ttctcctatg
     1441 tttttactat tgttctcaag gaacaaatac agtatctcaa aaaagcgcgt aatcgtcaac
     1501 aattattata aaatatattt ataaaaaata tttaccaaaa atgttgatat ttattgctgc
     1561 ccttgttata ttgttcttgt cccaatgaca catagtaaaa acataggaga acacaatgac
     1621 tattccagaa attatccagc aacaattgct gcgcacgaat aaagcgattg tttggtcttg
     1681 gggcgtatct aaatggtatg aactatcatc taatgcactt gcaataagag ttcatgcgcg
     1741 ttatttagat ggttttgtct gtatcgagtt ggatgaggcg caagacttat acaatatttc
     1801 cttttacctt aaccaagact tctcagacat actggtgtgg ccagtgatac cctataaacc
     1861 gatgaaagga gtctattgcg atcaactggt tgaattcatt gataacagaa tagaaagaat
     1921 acccgattat aaatactgac tattgcccta cttcctaccg tggggagtag gccattaaca
     1981 ggagtagatt catgaaaaaa ttatcgtttg ccgttaaagc aaacatgaat aaaccaccta
     2041 gggttcacgt gcaatcagcc gataaaaaat caacttatgg ctcatttcaa gcaaataact
     2101 gcgatgaatt cgatagttgg gacaaattaa gccaggacga agccattgaa ttaaagcact
     2161 acatgaataa tctagtggct attgagcatt acttttccac aaaggctctg tccgaacaaa
     2221 aagattttag gatccgatta cccggtagtt ttattgatgc gattgatgaa ctaagcaagt
     2281 tatgttttga agagcacatc gacttgaatg tttatgatgc catgataagt gccgctattg
     2341 ggcaacttaa aataaagaca gcaagccttc ctgatgaaaa aaaacagcaa gcgcttacac
     2401 tgcttaatca actaggatta tctgaaaatg taaaaacaga tgtttccttg aaaatacagg
     2461 cggtattctc tgaattatta tccattcata acaaatcaga aaaactgcac caaaaagcca
     2521 ggatgctttt taataaagat aaaagtattg ctccaaaaac tattgaggaa atcgcaaaag
     2581 gtgaactatc aacttccaaa tggttagtgg cttgtgctgt agagatttta ctggaagaaa
     2641 aaccagatat agtgcaaaaa atattaactg aggacgatat cttgtttttg tgggctaatc
     2701 ctttactaaa aaatcataga ccgctcaagg aactgctcga taaact
//
LOCUS       DAXLDT010000269         2702 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXLDT010000269 DAXLDT010000000
VERSION     DAXLDT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.270"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            77..760
                     /gene="lem2"
                     /locus_tag="ACF2SP_003048"
     CDS             77..760
                     /gene="lem2"
                     /locus_tag="ACF2SP_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem2"
                     /protein_id="HFD2360804.1"
                     /translation="MVIIGTLMSKERIKKAALEIVEYIHGNIKACNIPNQKKENKFFA
                     PLVYLCQTECDKILSNTKISLLERLLKCAKIIDDLQSGNCMQQTFLAFQRLLTRLIED
                     KLSNFSTCIPISVMTIANHAFLIIDNDIVCDPWLNFVGDLKDYCFANMKRKEYFGIRS
                     DWTCFTNTEVYDEDSTEFTIQFVNALGVGISGFSEVKLANQLMQSSSMASDLGEDSVI
                     PAPKGFHRI"
     gene            865..1638
                     /locus_tag="ACF2SP_003049"
     CDS             865..1638
                     /locus_tag="ACF2SP_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360805.1"
                     /translation="MNFLNNKIVMITGASSGIGQACARLFAAQGARLILAARRVERLH
                     ELSSELKHHNNQEHYILPLDVCKKDLVRQQIESLPSQWNSIDVLINNAGLALDTLPLH
                     QGILEHWDTMIDTNIKGLLYISRLILPGMLERNSGHVINIGSIAGHECYPNGNVYSAT
                     KHAVHAISKSMRLDMLGSAVRVTEIAPGAVETEFSEVRWKDKQRAIDFYKDFQPLMAE
                     DVADAALYCATRPLHVDIEQMIIMPTVQASANHLSRRGR"
     gene            1882..2241
                     /locus_tag="ACF2SP_003050"
     CDS             1882..2241
                     /locus_tag="ACF2SP_003050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360806.1"
                     /translation="MPTQVIRYFVLRWYIEVTFEEIWLILVLKRNANGRTRLLPEQHH
                     LLMGLFSFVILFAFNMYQTKALVSMEMASCYDKKGELTCSDILVIVRRSIWANIYFSK
                     SDNQLDSTKYSEKTSIH"
BASE COUNT          781 a          490 c          587 g          844 t
ORIGIN      
        1 gggatttgtt tttgtcaaat tgtaagcgat ttttgattta aaaaaggtat aataggtaac
       61 aaaagaaaaa ccacctatgg ttataattgg aacattgatg agcaaagaac gaattaagaa
      121 agcagcactg gagattgtcg aatacattca tggcaatata aaagcgtgta atatccccaa
      181 tcaaaaaaaa gaaaataaat tttttgcgcc tttggtttat ctatgtcaaa cagaatgcga
      241 taagattttg agtaacacca aaatcagttt gttggagcgt cttttaaaat gcgcaaaaat
      301 tatcgatgat ctgcaatcag gaaattgtat gcagcaaacc tttttagcat ttcaacgttt
      361 attgacgcga ttgattgagg ataagctatc taatttttcc acttgcattc caatttctgt
      421 tatgaccatt gccaaccatg cctttttaat cattgataac gatatcgtgt gtgacccctg
      481 gttaaatttt gtgggagatt tgaaagatta ttgttttgca aacatgaaaa ggaaggagta
      541 ttttggaata cgttccgact ggacttgttt tacgaataca gaggtctatg atgaagactc
      601 tactgaattt acaatccaat ttgtaaatgc tttaggggta gggatatcgg gtttcagtga
      661 agttaagttg gcaaatcaat tgatgcaatc atcgagtatg gcttctgatc ttggagagga
      721 ttctgtgatt cctgccccaa aagggtttca tcgaatttga tggagcgctc atcttgattg
      781 taatgaactc tattgctgtt gtttttgttc aagttctggt tcgatttaac ggtaaataca
      841 ttgtacttga tgatggggga gaggatgaat tttttaaaca ataaaattgt aatgataacc
      901 ggcgcgtcca gcggcattgg acaggcttgc gcccgacttt ttgctgcaca aggggcacgg
      961 ctcatcttgg cggcacgacg agttgaacgt ttacatgaat tatccagcga gttaaaacat
     1021 cataataatc aagaacatta tattcttcct ctggatgtct gtaagaaaga tttggtgaga
     1081 caacaaattg aatcgttgcc cagtcagtgg aactctattg atgtgttaat taataatgcg
     1141 ggtttggctc tggacacttt gcctttgcat caaggaatat tggagcattg ggacaccatg
     1201 atagatacca atattaaagg attgctttat attagccggc taattctccc agggatgtta
     1261 gaaaggaata gtgggcacgt cattaatatt ggttctatag caggtcatga gtgttatcct
     1321 aatggaaatg tttactcggc caccaagcat gctgttcatg cgatatcaaa gtctatgcga
     1381 ttggatatgt taggaagtgc agttcgagtg actgaaattg caccaggtgc tgttgagaca
     1441 gagtttagcg aggttcgctg gaaagacaaa cagcgagcta ttgatttcta taaggatttt
     1501 caaccgctta tggccgaaga tgttgctgat gctgctttgt attgcgccac aagacctctg
     1561 catgtcgata ttgagcaaat gattattatg ccaacagttc aggcttctgc aaatcatttg
     1621 tcaaggagag gcagataact cgactttagc cattttccag aaccgttcgc cctgaggagc
     1681 gtgcatagca cgcgtctcga agggttctca cttaaaagct atttcgattt tcgtttagcg
     1741 tagtagatag ttgccccaat cacctgagct caaagcctcc ttcttttttc tgcttcatcc
     1801 ttgaatttgc ttttcggcgc tcaatccctc tattctgcta gaatattgct ctgagtacac
     1861 aagcttaacg gtacggctat aatgccaacc caagttatcc gctacttcgt gcttcgttgg
     1921 tacatagagg tcactttcga agaaatctgg ctcatcttgg tattgaaacg caacgccaat
     1981 ggccggacaa ggctattgcc agaacaacac catttgctaa tgggattatt ttcttttgtc
     2041 atattgtttg ctttcaacat gtatcagact aaagcgcttg tcagtatgga gatggcctct
     2101 tgctacgata aaaaggggga gcttacttgc tctgatatcc ttgtgattgt caggcgatca
     2161 atttgggcaa atatatattt ttcaaaatct gacaatcagc ttgattcgac taaatactca
     2221 gaaaaaacat cgatacattg atttatcagc tctctttagc tccctgactg acccttcgag
     2281 acgcgtgcta tgcacgctcc tcagggcgaa cggttctggg aaaatggcta aagtcgagag
     2341 ataatcatca ggtgaataat tgctcaaaaa tttgccaaca atggcgctgt tagtctatta
     2401 tttaaaatat aggtgatgac tgatcctggc tgaaccagta agtagtgcca gattaggagg
     2461 tcgttttgga gggtggtgtg taagcctaca aagggtagga aacctgaagc tctacataaa
     2521 tctccacagt gtctttcagt tgcggctcag aaaccgggaa gtcgttgcct gtacttaaac
     2581 gcttttctat tctccgtata aactaatttt tttcttatta aaggatgagt taagtcgaac
     2641 agataataga caaacccaca atcgccttgt taaaaatgtt tttaatttag tcatttggtt
     2701 ta
//
LOCUS       DAXLDT010000270         2692 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXLDT010000270 DAXLDT010000000
VERSION     DAXLDT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.271"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..435)
                     /locus_tag="ACF2SP_003051"
     CDS             complement(<1..435)
                     /locus_tag="ACF2SP_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HFD2360807.1"
                     /translation="MIPWNAYVRLLRLNKPIGILLLWYPTAWALWMANQGFPSIDLLM
                     IFLLGTVFMRSAGCVINDIADRHIDRHVARTQFRPLTSGEVSLSEAFILLFILLCASL
                     LLLLKLPINCFYFAVISVLITFVYPFCKRFLNAPQLILGLAFS"
     gene            complement(432..968)
                     /locus_tag="ACF2SP_003052"
     CDS             complement(432..968)
                     /locus_tag="ACF2SP_003052"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFD2360808.1"
                     /translation="MSINYQSLLIADTKGPGLLKDWLEYQGSLTDKLKAMTGNAELER
                     LSQNWSIPNWWDKYVLCIQDKSILQREIVMKNQGIVYWYARSVIPQSCYALKPEFFNR
                     LENESIRNLIFDESSVRRLPILCYPVDQLNLEFHWVKKYIGSEYSQMWVRFTELVFQE
                     KSSFYLVEILLPELESLL"
     gene            complement(1085..1567)
                     /locus_tag="ACF2SP_003053"
     CDS             complement(1085..1567)
                     /locus_tag="ACF2SP_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFD2360809.1"
                     /translation="MRLQLMKITGFTLLETMLALALLLGLLLLGSWSFFSLIQNNERE
                     SLVNSIKTAIQYSKIQAIHLGHPIYLLPFGSNENWSRGMVLAILNRKSNKTELIYQWQ
                     WSSNSWNINWKGVDSNHRIIISNIPNRAMSNGKFILNNRRTNEKIVVTLNRLGRVRVG
                     "
     gene            complement(1573..2352)
                     /locus_tag="ACF2SP_003054"
     CDS             complement(1573..2352)
                     /locus_tag="ACF2SP_003054"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360810.1"
                     /translation="MMTLITGATRGIGLALAYEFASHGHDLILIGRDSEQLEHISKQL
                     NTQYQVNVEYKVHDLSKPGSAFKLYTDLKEQGIEVNCLVNNAGIGYMGSFVEMGMNKL
                     DDLMNINMISLSELTLCFLHDFVARNEGSILQVSSTAAFQPGPFMAAYYASKAYVATL
                     SHAIAYELKDTKVSMSILCPGATKTNFFHASGMENSMLERGYIGMMNPEKVAKIAYKG
                     LKKGKLYIIPGLRNKILAYAAAISPKAIALRIASYLHHKIL"
     gene            complement(2297..>2692)
                     /locus_tag="ACF2SP_003055"
     CDS             complement(2297..>2692)
                     /locus_tag="ACF2SP_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HFD2360811.1"
                     /translation="SLNIHSPFDCLEHFNKISGNAHVMIHIIVKLIKELKERTCQQIY
                     DVLKEVRHLLYSDINLMKANYFGKWDLSLPQCIRILNLLKQEKHDDVSRQLDCLRNQM
                     EPVQTVKGGVHHDDLNNGGHERYRLGLGV"
BASE COUNT          911 a          522 c          421 g          838 t
ORIGIN      
        1 ggaaaaagcc aaacctaata tcagttgtgg agcattcaaa aatcgtttac aaaatgggta
       61 tacaaaagta attagcacgg aaataaccgc aaaataaaaa caattgattg gaagttttaa
      121 taataaaagc aatgaggcac aaagtaatat aaacaataaa ataaatgcct cagacaagct
      181 gacctctcca gaagtcaagg ggcgaaattg ggttcgggct acatgcctat caatatgcct
      241 gtcagcaata tcgttaataa cacaacctgc ggaacgcatg aatactgtac ctaataaaaa
      301 aatcataagc aaatcaatag aaggaaagcc ctgatttgcc atccaaagtg cccatgcggt
      361 gggataccaa agtaataaaa ttcctattgg tttatttaag cgtaataatc tgacataggc
      421 attccaggga atcataataa actttccaac tccggtaaca agatttcaac caaataaaaa
      481 gaactctttt cctgaaatac tagctccgta aacctgaccc acatctgaga gtattcagag
      541 ccaatgtatt ttttaaccca atgaaactca agatttaatt gatctacagg ataacaaagt
      601 attggtaacc gcctcacact actttcatca aaaatgagat ttcgtattga ttcattttcc
      661 aaacggttga aaaattctgg cttaagcgca taacacgatt gtgggataac cgatcgcgca
      721 taccaataga caatcccttg atttttcata acaatttcac gttgaagaat gcttttgtcc
      781 tgtatacaca gaacatactt atcccaccaa tttgggatag accagttctg agataaacgt
      841 tccagctccg cattaccggt cattgctttt agtttatctg ttaaggaacc ttggtattct
      901 aaccaatctt tgagtaatcc tggtcctttg gtatcggcaa tcagcaggga ttgataatta
      961 atagacatac ataaataatt tggaaaaaaa tttcataaga tattacaaaa ttacctcttt
     1021 atgaacaacc attgcttata atttaagata aaaacaaaat tattttattt tgattttgtg
     1081 ttaattaccc caccctaact ctaccaagcc tatttaaagt aaccacaatt ttttcattgg
     1141 ttcgtctatt attcaaaata aatttaccat tgctcatcgc acgattcgga atattggata
     1201 taataattct atggtttgaa tctacgcctt tccaattaat attccaggaa ttagaactcc
     1261 attgccattg gtaaataagc tcggttttat tggattttcg attaagtata gcgagcacca
     1321 tccccctgga ccaattttca ttagaaccaa aaggaagaag ataaatcgga tgtcccaaat
     1381 gaatagcctg tattttagag tattgaatgg ctgtttttat actattaact aatgattctc
     1441 tctcattgtt ttgtattagt gaaaaaaatg accatgaacc taaaagcaat aatcctaaaa
     1501 gcaaagctaa tgcaagcatc gtttcaagta gagtaaatcc tgttattttc atcaattgaa
     1561 gccgcatatc atctacaaaa ttttatgatg aagataagat gcgatcctaa gagcaatcgc
     1621 tttgggtgat atagctgcgg cataggctaa aattttattt cttagccccg gaataatata
     1681 caactttcct ttttttaatc ctttataggc aattttagcg accttttcag ggttcatcat
     1741 tccaatatat cccctctcca acatagaatt ttccatacca gaagcatgga aaaaattggt
     1801 ttttgttgct ccaggacaaa gaatagacat agatacctta gtatctttaa gctcataagc
     1861 aatagcatga gaaagcgttg ctacatatgc cttactcgca tagtaggctg ccataaaagg
     1921 acccggttga aatgctgctg ttgaagaaac ctgtaaaata ctaccttcgt ttcgagctac
     1981 aaaatcatga agaaaacata gtgttaattc acttaaagaa atcatgttta tgttcattaa
     2041 atcatcgagc ttgttcatgc ccatttcaac aaaagaaccc atatagccta tgcccgcatt
     2101 attaaccagg cagttgactt ctataccctg ctcctttaaa tcagtataca acttaaatgc
     2161 gcttccaggt ttacttaagt catgtacttt atattcaaca ttaacttgat attgtgtatt
     2221 taattgcttt gaaatgtgct ccagctgttc agagtctcta ccaataagta ttaaatcatg
     2281 gccatgtgat gcaaactcat acgccaaggc caagcctata cctctcgtgg cccccgttat
     2341 taaggtcatc atgatgcact cctcctttta ctgtttgcac aggctccatt tggttacgta
     2401 ggcaatccaa ttgccgagat acatcgtcat gcttttcctg ttttaacaaa ttcaagatcc
     2461 gtatacattg agggagactt aaatcccatt tgccaaaata atttgctttc ataagattaa
     2521 tatcgctata caataaatgc ctaacctctt ttaatacatc ataaatttgt tggcatgttc
     2581 tttctttgag ctctttaatt aatttaacga tgatatgaat cataacatga gcatttcctg
     2641 aaattttatt aaaatgctca agacaatcaa agggagagtg tatatttagt ga
//
LOCUS       DAXLDT010000271         2685 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXLDT010000271 DAXLDT010000000
VERSION     DAXLDT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.272"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(367..855)
                     /locus_tag="ACF2SP_003056"
                     /pseudo
     CDS             complement(367..855)
                     /locus_tag="ACF2SP_003056"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(836..1087)
                     /locus_tag="ACF2SP_003057"
     CDS             complement(836..1087)
                     /locus_tag="ACF2SP_003057"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2360812.1"
                     /translation="MIFPIIISSHAPKCRHGENDRLFIEAVCWIIRTGAPWRDLPPDY
                     GKWQSVYGRYNRWVKNGHFNGILEILKKRWRSRMAHDGW"
     gene            1125..1463
                     /locus_tag="ACF2SP_003058"
     CDS             1125..1463
                     /locus_tag="ACF2SP_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2360813.1"
                     /translation="MYPVENLDRAMDFYKKNLGLIPSKISANGHWIEYDLPQGGCFAI
                     TTLAEGVKPSAVSGGSIAFEVEGLDSLTQQLKKNGVTFKLDTFSSPVCRMAIIIDSEG
                     NAITLHELNQ"
     gene            1483..1890
                     /locus_tag="ACF2SP_003059"
     CDS             1483..1890
                     /locus_tag="ACF2SP_003059"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFD2360814.1"
                     /translation="MMMFAKCSVFIATSLDGFIARDNGSIDWLMKANELVPPGEDGGY
                     KEFISTVDGLIMGRHSFEKVLSFDPWPYGELPVIVMSSKPIEIPAHLKYVSASSETPI
                     ELVKRLTDEGFKHLYIDGGITVQNFIAANLIHE"
     gene            1982..2491
                     /locus_tag="ACF2SP_003060"
     CDS             1982..2491
                     /locus_tag="ACF2SP_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFD2360815.1"
                     /translation="MYWRWFCSVEISDGCGTGEPIAQFLIKQGYKVTGIDASQKMIEQ
                     YKIRFPDAKWLLADMRTLDLQEKFDAVIAWHSFFHLPHDDQRKTLKSLASLVEQNGLL
                     IFTSGPEYGEVWGDNGGYDLYHASLSSEEYTQILLKCHFKVLVHNIRDPDCGEATVWV
                     AQKNHITPE"
BASE COUNT          849 a          492 c          490 g          854 t
ORIGIN      
        1 tgcttaagtt acagaaacgg agcaaatgtg aactagaacc tcctttctgt aaaatagagg
       61 ctaataaagg cagataatcg caaaatatcc cacacaataa atacccagcc aaaaatttct
      121 ttgaaaactt ctcgtgaact tttaataaaa gggtcccaaa aatgcttgaa taaatacatg
      181 atgttcatgt gacataaagt tgttatagta ttttcataca gatagataac caaagtcttt
      241 ctttattagt aattaatttt ccaaatactg ctagaaaaac aaatagttct attcaatgtc
      301 tagagtgaat tctttaaaaa aatttatttt taggtaacaa tggcacttta atactagggc
      361 gtgtttttaa agtttcagcc acaatataat acttactaga gttagtgctc ctagaaacat
      421 gatagctgtt ttatcataat gtgtagcaat tctcctaaaa cttttgactc gattaaaaaa
      481 acgctcaacg taattacgct ctttgtatat atgtgtatca tgctctatag gtatgatgcg
      541 attctttttg gatgggatga ccgccgtgat tcctctattt ttcaactccc cgcgaaattc
      601 attgctatca taagccttat cggctaataa atactcggat agttcatcac cgagcaattc
      661 ctttgctgtt tttatctcac tgtcttgtcc aggtgttaaa ataaaactca aaggcaatcc
      721 aaaagaatca acctttacat ggatttttga gctaaaacca cctttgcttc ggcctagtgc
      781 ttgtttctct tggccgcctt gagctccagc cgcatgttga tgtgctcgaa taacgctacc
      841 atccatcatg tgccattcgt gatcgccatc ttttttttaa aatttcaagt attccattga
      901 aatgtccatt tttaacccat cgattatagc gaccataaac actttgccat tttccataat
      961 caggtggcaa atctctccaa ggcgcgccag tcctgataat ccagcaaacc gcttcgataa
     1021 ataatcgatc attttcacca tgacgacatt tgggcgcatg tgagcttata ataatgggaa
     1081 aaattattta aaggagaaac catgttaaaa aagtagcgtt taccatgtat cctgtcgaaa
     1141 atttagatcg agcgatggat ttttataaaa aaaatcttgg tttaatacct agcaaaatat
     1201 ctgcgaatgg tcattggata gagtacgact taccccaagg cggatgcttt gcaatcacaa
     1261 cccttgcgga aggtgttaaa ccaagtgctg tgtccggggg tagtattgct tttgaagtgg
     1321 aaggtttgga ttccttaacg caacaattaa aaaagaatgg ggtaacattc aaattggata
     1381 ctttttcatc acccgtatgt agaatggcaa ttatcatcga ttctgaagga aatgctatta
     1441 cactgcatga gctcaatcaa taagtctgta tacaaaggat aaatgatgat gtttgctaaa
     1501 tgttctgtat ttattgctac aagtcttgat ggctttattg caagagataa tggttctatt
     1561 gattggctaa tgaaagcaaa tgagcttgtg cccccaggtg aggatggtgg ttataaagag
     1621 ttcatatcta cggtcgatgg actcattatg gggaggcatt cttttgagaa agtcttatca
     1681 ttcgaccctt ggccttatgg tgagcttcct gtcatagtga tgagtagcaa acctatagaa
     1741 attcctgctc atttaaaata tgtttcagca tcttccgaaa cacccatcga gctcgttaag
     1801 cggttaactg atgaaggatt taagcatctt tatattgatg gaggaataac tgtccaaaat
     1861 tttatagccg cgaatttaat ccatgaataa caataaccat cgcaccagtc ttgctaggtt
     1921 ctggtcgttc attatttgta catttgacac aagacattga actggagcat cttgaaacca
     1981 aatgtattgg cggtggtttt gttcagttga aatatcggat ggctgcggta caggcgaacc
     2041 catagcacaa tttttaatta aacaaggata taaagtaacg ggcattgatg caagtcaaaa
     2101 aatgattgag caatacaaga taagattccc agatgcaaaa tggctacttg ctgatatgag
     2161 aactttagat ttacaagaga aattcgatgc ggtcattgcc tggcatagtt tctttcattt
     2221 acctcatgat gatcaacgaa aaacattaaa atctcttgct tctttggtag agcaaaatgg
     2281 tttattaata tttacttccg gacccgagta cggggaagtc tggggtgata atgggggata
     2341 tgatttatat catgcatccc tttcgtctga agaatatact caaattcttc taaagtgcca
     2401 ttttaaagtc cttgtacata acattaggga tcctgattgt ggggaagcaa ctgtttgggt
     2461 tgctcaaaaa aaccacatta ctccagagta aaatgaaaaa aacttcttca aatcttatac
     2521 tcaaaaataa tgggatatct atttggcatc ataggagatt ttttaacacc aattttgatg
     2581 catcccattt tcttgtaaaa tggttcagca ccaggatcgc tccataattt gaatctacta
     2641 acaccatttg atttaaaatc ttctaccatc atagaccaca ttttt
//
LOCUS       DAXLDT010000272         2659 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXLDT010000272 DAXLDT010000000
VERSION     DAXLDT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.273"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(185..307)
                     /locus_tag="ACF2SP_003061"
     CDS             complement(185..307)
                     /locus_tag="ACF2SP_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFD2360816.1"
                     /translation="MTKYRDAGTGRYVTKEFAKKHPGTTVGENSKPKSQRKHKK"
     gene            452..1147
                     /locus_tag="ACF2SP_003062"
     CDS             452..1147
                     /locus_tag="ACF2SP_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360817.1"
                     /translation="MARFFKDREIEIKDSAGEYYKKLCNALKTTMLVCIASTSALVIN
                     ELTAKINKDRMTQYEENSSVAIQGIGEIRVFLLRIYKHLEDNKIDFLDGRAAIKNTMS
                     KGMAGNCEHQAFYLAALLRQQNIPALIYDIEDIGHTVVITQNYLLDPWVGEIFALVKT
                     DLGKFFGSSLNMKASWLNRLLSNKKFSYPDELTLHTLNEYFVQQEDQYRIGLNILDNK
                     EGMDHQMSCELAY"
     gene            complement(1241..1879)
                     /locus_tag="ACF2SP_003063"
     CDS             complement(1241..1879)
                     /locus_tag="ACF2SP_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360818.1"
                     /translation="MPTYFDPIMQEDTVLDENTIVYLVKIGDNKFSIKAISSGLEHLP
                     SDPTTHAEQYWPIPAKSLIDHSSNKLLFEEDKLTNQPISKGQVIELFAVDPDKTEPKQ
                     FSDSVKRELTENWAREVLQDQFAHAGQNPHFFFYQPLFIHGHHGDNTAQQQDNAMPIP
                     LPLLMFSLIPPMNNNNQQQMALLFFNILLVPQDQGHEPGQENEGEHAPSNGH"
     gene            complement(2095..2658)
                     /locus_tag="ACF2SP_003064"
     CDS             complement(2095..2658)
                     /locus_tag="ACF2SP_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360819.1"
                     /translation="MSSYAVYNTWGLNDCPEQLWSKVSISAVKKETGSSFVHLNGPRY
                     WVIDGFKNTSLINPAIKTISGIPMREAGILHLSLMDLFKNKPYQSHVVDRKTTWVYQA
                     DKPVFELIDPNGQVFVMQSYSVQKYPQTMNTLTQLGAKLQLPKGWKFKTGVLNKPETI
                     EAVNNKAVVVQDNFLNTYQKASHDFLK"
BASE COUNT          796 a          466 c          522 g          875 t
ORIGIN      
        1 tttatattgc tggatttatg ttaaaacatc aatgtttaaa tgcgatatac aagttctgtt
       61 ttatgaaaat tcttcacctt ttatgattta aaatatcaag aaactctctt tatggcctat
      121 tttaaggaca acatgattag tctcttgcgt caaacgcaac caaagggcaa ttaccccatg
      181 gcgattattt tttatgtttt ctttgtgatt ttggtttact attttcacca acggttgttc
      241 cgggatgttt ctttgcaaac tctttggtga cgtatcgacc ggttcctgca tctctatatt
      301 ttgtcatttt aaactccatt tttatccgct ccaatggaag tatagcaaat tcagtcagca
      361 agaggagttt tggtgaaaaa gaggctgttc tacttgctcc tctcatactt gtatgtaaac
      421 taaaaccctt attggatata agggattaga tatggccaga tttttcaaag acagggaaat
      481 tgaaatcaaa gattcagcag gcgagtatta caaaaaactg tgtaatgctc taaaaacaac
      541 catgttagtg tgcattgcca gcacttctgc attagttatt aatgaactca ctgcaaaaat
      601 caataaagat agaatgactc aatatgagga aaatagctct gttgctattc agggtatcgg
      661 tgagataaga gtttttctac tcaggatata caaacatctt gaagacaaca agattgattt
      721 tctggatggg agggcggcaa taaaaaacac aatgtcaaag ggaatggcag gaaattgcga
      781 acaccaggct ttttatcttg ctgccttgct gagacaacaa aatatccctg cgcttattta
      841 tgatattgaa gacattggcc atactgtagt tatcactcaa aattatctcc ttgatccatg
      901 ggtaggtgag atttttgcac ttgtcaaaac tgatctcggc aaatttttcg ggtcgtcact
      961 gaatatgaaa gcctcgtggc taaatcgatt attaagtaat aaaaaattca gctaccctga
     1021 tgaacttact ctccacacat taaacgaata ttttgtacaa caagaagacc aatatcgaat
     1081 cggacttaat attttagata ataaagaggg tatggatcat caaatgtctt gcgaactagc
     1141 ttattaaaaa agcattgccg cctgaatgca cctgtcaaaa tagaaatgtc gggcgcggtg
     1201 cccgacttaa tcttgttaga aatgcaagtt aggtttaatg tcagtgccca ttagaaggag
     1261 catgctcgcc ttcattttcc tgcccaggtt catggccttg atcctgtggt accaggagta
     1321 tgttaaaaaa taataatgcc atttgttgtt ggttattatt attcatgggt ggtatcaagg
     1381 aaaacatcag gagtgggaga ggaattggca tcgcattatc ctgttgttga gcagtgttgt
     1441 ctccatgatg cccatggatg aataggggtt gataaaaaaa gaaatgaggg ttttgccctg
     1501 catgagcaaa ttgatcctgt agtacctctc gtgcccagtt ttcagttaac tctctcttaa
     1561 cggagtcact aaactgcttg ggttcagtct tatcgggatc aactgcaaaa agttctatta
     1621 cttgaccttt gcttatgggt tgattggtta acttatcctc ttcaaaaagg agtttgttac
     1681 ttgaatgatc aatcaatgat ttggcaggaa taggccaata ttgctccgca tgagtagttg
     1741 gatcactggg gaggtgctcc agtcctgatg aaattgcttt aatactgaat ttattatcac
     1801 caatcttaac taagtaaacg atcgtgtttt catccagcac agtatcttct tgcataatag
     1861 gatcaaaata agttggcata ataatagtcc tttagctgtt atttaaactc aatagtaact
     1921 tataggatca aattggcttt tgcaatactt ttgattcatt atttgatcaa gcaatatgat
     1981 ttggtttgca aacagtttgg attaaataca atgactgaca cttgtcagtt ctcaatgtag
     2041 gcacagggca agatttaatc ttttgccctg tgacgctctt tccgcatgct aaatttattt
     2101 cagaaaatca tgactggctt tttgataggt gttcaaaaaa ttatcttgga caactacggc
     2161 tttattatta acagcttcaa ttgtttctgg cttgttaagc acaccagttt taaatttcca
     2221 acctttggga agctgaagtt tggcgcctag ttgtgtgagg gtattcattg tttgtgggta
     2281 tttttgaaca ctataggatt gcattacaaa aacttgtccg ttgggatcaa tcaattcaaa
     2341 gacaggttta tctgcttgat agacccatgt tgttttgcgg tccacgacat gagattggta
     2401 aggtttattt ttaaataaat ccattaaact aaggtgcaaa atgcctgctt cacgcattgg
     2461 aattccacta attgttttga tggctggatt tatcaaagaa gtgtttttga atccgtcaat
     2521 tacccaatat cgagggccat tgagatgaac aaaagaagag ccagtctctt tttttactgc
     2581 ggaaatagaa actttactcc acaattgttc cgggcaatca tttaatcccc aagtattata
     2641 cacggcataa ctggatatg
//
LOCUS       DAXLDT010000273         2619 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXLDT010000273 DAXLDT010000000
VERSION     DAXLDT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.274"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            47..1510
                     /locus_tag="ACF2SP_003065"
     CDS             47..1510
                     /locus_tag="ACF2SP_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD2360820.1"
                     /translation="MKTNIPTLSKPIKDSGYRRGLKDRHVQLIALGGIIGSGYFLGTG
                     EVINLVGPSVFIAYLLGGLIIYLTMLCMGELAVAIPISGSFVTYTSDFISPTVACGVG
                     WSYWITWVAYIPAECVAGGIIMELFTGVSGYIWVVCFGLIITYINLAKVDTFGEIEFW
                     LALIKIISLLAFVFLAILIFFGLIHGSEPPGIIGFKYLLGDGGLLPNGAMSLLTAMVL
                     LLVNYQGSEIIGLAAGESENPARMIPHAIRNVTFRILFLYIIPVFCLVLIFPWQKAGL
                     SNSVFADALNFYGLKWAGAVTSFVTLSATLSCANSGFYGAVRSLNALARDGMAPHVLA
                     KFNQNSVPQNAVIATLIGVWILLGVGYFFGQTKLYIALLLVSGFTGTLAWLSLCIAQI
                     SFRNRLYKAGYSIKDLRYVTPYSPYTGILAVILMVGSLFFLLLNKDPIYKLSFIIGVV
                     SFIIPVIIYKVFDLSKVRKKALHVKSRVKFQDLFPPQ"
     gene            complement(1807..2547)
                     /gene="ravK"
                     /locus_tag="ACF2SP_003066"
     CDS             complement(1807..2547)
                     /gene="ravK"
                     /locus_tag="ACF2SP_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavK"
                     /protein_id="HFD2360821.1"
                     /translation="MVSLEHIQKLISECRKLGKDGLDNGTNGLIPELEIDVVPPSAFL
                     GVGNNPAIFVNSKTYKLMRTTHEKWVENKTIVFKSYLLSQPAIKIIGAIVHETGHAFN
                     VAAKIPNTEANACIFEIEVLMRLFQVKSPLLLGCTELDMQSYFKSRLTDYNKCVKDCR
                     CLAEMVQLITHQFKLDEVSISEKENQIPLLSISNKWPGLFAKKQVAPDMDKLLTSPVT
                     ITPEVKILFYQLIKERFYSPETEIKLGI"
BASE COUNT          725 a          522 c          489 g          883 t
ORIGIN      
        1 cattagctct attatttttt actaacatag tgagaaaaaa cagcccgtga agactaatat
       61 tccaacccta tcaaaaccca ttaaagacag tggttacaga cgaggtttaa aagatcgtca
      121 tgtccaactg attgcactag gtggaattat cggctcagga tattttttag gaaccggtga
      181 agtaattaat cttgttggcc cctctgtgtt tattgcctat ttactaggtg ggttaattat
      241 ttacctaacc atgttatgta tgggtgaatt agcagtcgcc attccaattt ccggctcttt
      301 tgttacttac acttctgatt ttatatcccc aactgtcgct tgcggtgtcg ggtggtcata
      361 ttggattacc tgggtcgcct atatccccgc agaatgtgtt gcgggaggga tcataatgga
      421 attgtttact ggggtgagcg gttacatatg ggtggtgtgc ttcggattaa ttattaccta
      481 tattaacctt gctaaagtag atacgtttgg tgaaattgaa ttctggctcg ctttaattaa
      541 aattatctca ttactcgcct ttgttttctt ggcaatacta attttctttg gcctcattca
      601 tggatcagaa ccacccggta taataggatt taagtacctg ttaggcgatg gaggtctgtt
      661 acctaatgga gcaatgtcgt tgttgacagc catggttctt ttgctggtta actaccaagg
      721 atctgaaatc ataggacttg cagcgggaga atctgaaaac cctgcccgaa tgatccctca
      781 cgccatccgt aacgttactt tcagaatcct gtttctctac atcattccag tattttgcct
      841 ggttttaatt tttccctggc aaaaggctgg gttatccaac tcagtcttcg ctgatgcgct
      901 taatttctac ggcctgaaat gggcaggagc agtaacaagt tttgtcactc tatctgcaac
      961 cttatcctgc gctaactccg gattttatgg tgctgtgcgt tctttaaatg ctttggcacg
     1021 cgatggtatg gctccacacg ttttagccaa atttaatcaa aactctgttc ctcagaacgc
     1081 cgttattgcc acactgattg gagtttggat tcttttaggt gtgggttact tttttggtca
     1141 aacgaaactc tatatcgctt tattattagt atccggtttt accggaacgc tggcttggct
     1201 ttctttatgc attgctcaaa tcagctttag aaaccgtttg tataaagccg gttactccat
     1261 taaagatctc cgttatgtaa caccttattc accctatact ggaattcttg ctgttatttt
     1321 gatggttggc tccttatttt tcttactgct aaataaagat cccatctata aattatcatt
     1381 tattattggt gtggtgagtt tcataatacc tgtcatcatc tataaggtct ttgatttatc
     1441 caaggttagg aaaaaagctt tgcatgtgaa aagtcgggtg aaatttcagg atttgtttcc
     1501 gccacagtaa attaaacatt actttttgag taaccgtaca cggggtatgc aaatttttct
     1561 gtttataagc ctatttattt gagttgtctt tcccgcgaag gcgggaagcc aactaaaaat
     1621 caacccttgt gctagattta tggatagatt cccgccttcg cgggaaagac agaggaccta
     1681 atattcctta attacatacc ccatgaacag ttaccttttt gaagtgtttt gggcgtgcca
     1741 gaatttgttt tgcacgcctc actcataaag tcacagtaat attacctgca ttaatcaaaa
     1801 atattgttat ataccaagct ttatctctgt ttcaggagaa taaaaacgct ctttaatcaa
     1861 ttgataaaac aggattttaa cctctggagt gatagtcaca ggagaagtca ataatttatc
     1921 catgtcaggg gcaacctgtt tctttgcaaa taagcctggc catttgtttg aaatagacag
     1981 aagcgggatt tgattttctt tctctgaaat tgatacctca tccagtttaa attgatgagt
     2041 tattaattgg accatttcag ctaaacatcg gcagtctttc acacatttgt tgtaatcagt
     2101 taaccgtgat ttaaaataag attgcatatc cagttccgta caccctagca gcaaggggct
     2161 tttaacttga aacaacctca ttaacacttc aatttcaaaa atacaggcat ttgcttcagt
     2221 attgggaatt ttggcagcca cattaaaagc atgaccagtt tcatgaacaa tagcaccaat
     2281 aattttgatc gccggctggg aaagtaaata acttttaaaa actattgttt tgttttcaac
     2341 ccatttttca tgggttgtac gcattaactt gtatgtttta gaatttacaa aaattgccgg
     2401 attattacct acccctaaga acgcactggg agggacaaca tcgatttcca attcagggat
     2461 taacccatta gtaccattat caagtccatc tttacccaac ttcctacatt ccgaaataag
     2521 tttttgtata tgctccaaac ttaccataat acccctcctt attgtaagaa atccctgcga
     2581 tcataaagaa atcaaagata gcaaactgat caaaatatg
//
LOCUS       DAXLDT010000274         2607 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXLDT010000274 DAXLDT010000000
VERSION     DAXLDT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.275"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1426
                     /locus_tag="ACF2SP_003067"
     CDS             <1..1426
                     /locus_tag="ACF2SP_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg2382 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360822.1"
                     /translation="KALNFLNRIERCLVPQSNPQIMNQLLSALGLDKLTTGNEEYKFF
                     IQTIKKKSIDLNPLVSKIKSDILNNDPLWTLLDYIEGNELIDDAEIENASSTIQLQIN
                     LLCLLEAITITMANSNDFAHDIYNHMMKQRGKGATGNPFYNFLFGVPSRATLFERLKL
                     ISIDPGLTSIIFHRMMGANPETQADLDDFVSQNRLSGWNADIELANFDVASTSTLPAM
                     GVNILEAVWEDATGHNKNTGGILNAQAGLGLIRVMEEQEYTTSFKLAETTLPQGTTLH
                     SDFGYSLIPELKVDNSPKKVSQFHIDKQWMNLYNSWNLLFVIGNIDSVFVPLKLLMPS
                     VFSAEPSNYKETRVLTLFLVANLFLNQQTLNNPLFSNNFSFRNGTVIMKKWGEINKNH
                     ASELLQQCKELDDLYNHVLGKNPHLNFGLKLFSYFQDSHQFRLTKKAQPSTSIDYSFF
                     SGNTPSENNSEKMGVALHSSLYTK"
     gene            1672..2580
                     /locus_tag="ACF2SP_003068"
     CDS             1672..2580
                     /locus_tag="ACF2SP_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2360823.1"
                     /translation="MNILSCLKGFITTVDCKSFSKAAQKLYISPSKLSKQITWLEEEL
                     KVTLLIRSTTRLTVTDSGHRLYEKSLQLFEQLEAVKVIATPDQLELNGVIRLYLTVTP
                     AIPYLTTLSIQFMRQYPKIEVDIIVGSDTKELYSSTFDLAISFDDVKHPKLVCKRLFS
                     IQRKVFASPGYIAQHGLPQTLDELPKHNCLINTLYGLQNKWVFNKKIIHVSGNFKSNN
                     AGVLKQAAVADVGILWAPYFSVYEEIKNEALIPVLPHESSPDINLYAICPKPTIDEQK
                     INLLLNFFCEKALSENIASSFIDENN"
BASE COUNT          872 a          460 c          438 g          837 t
ORIGIN      
        1 aaaggcatta aattttctta accgtattga acgatgtctt gtaccccaat caaatcctca
       61 aataatgaat caattattat cagctcttgg gcttgataag ctaaccactg gtaatgaaga
      121 atataagttt tttatccaaa caattaaaaa gaaaagcatt gatctgaatc cgttagttag
      181 caagatcaaa tcagatattt taaataatga ccctttatgg actttgcttg actacatcga
      241 aggaaacgaa ttaattgatg acgctgaaat cgaaaacgct tcttctacga tacagcttca
      301 aattaattta ttatgccttt tagaggcgat aaccattaca atggctaaca gtaacgattt
      361 tgctcatgat atttataacc atatgatgaa gcaaagagga aaaggcgcaa caggtaatcc
      421 tttttacaat tttttatttg gagtaccttc tcgtgccacc ttatttgaaa gattaaaact
      481 gatttccata gatcctggtt tgacaagcat tatattccac cgaatgatgg gagcaaatcc
      541 cgaaactcaa gctgatcttg atgattttgt aagtcaaaat cgtttatccg ggtggaatgc
      601 tgatatagaa cttgctaatt ttgatgttgc atcaacaagt actttacccg ctatgggagt
      661 taatatttta gaagcggtgt gggaagacgc aacaggccac aataaaaata ccggtggtat
      721 tttaaatgca caagccggtt tgggattaat tcgcgtcatg gaagaacaag aatacaccac
      781 ttcttttaaa ttagctgaaa ctactttgcc tcagggcact acacttcatt cagattttgg
      841 ttattcattg atacctgagt taaaagttga taactcacct aaaaaagtat ctcagtttca
      901 tatcgataaa caatggatga atttatacaa tagttggaat ttattgtttg tgattggaaa
      961 tattgattct gtatttgtac cacttaagtt gttaatgccc agtgtgtttt cagcggaacc
     1021 atcaaattat aaagaaacga gagtgctcac cttatttctt gttgctaatc tcttcttaaa
     1081 tcaacaaacg cttaataacc ctctgttttc gaataatttt tcctttcgaa atggaactgt
     1141 cattatgaaa aaatggggtg aaatcaataa aaatcatgcc agtgaactct tgcaacaatg
     1201 caaagagctg gatgatttat ataatcacgt actcggtaaa aaccctcact taaactttgg
     1261 attaaaactt ttctcatatt ttcaggattc acatcaattt agactcacta aaaaagcaca
     1321 accaagtaca tccattgatt attcattttt ctcaggtaat accccatctg agaataattc
     1381 agagaaaatg ggtgtagccc ttcatagttc tttatataca aaataaaatg ttatgcttta
     1441 tacccagcaa ataaatagca gaatcggaga caaattatag atggtctccg tttttgagaa
     1501 aaaatttctt tctgaaatct ttcacgggga tacttgacga tccgttttaa ctgctgtttt
     1561 catggaataa actaataggg ttggtctatg tcagtaggta tgcggttgat ttacaaaact
     1621 ggtatatagg tctataccat ctaaaaagag ctcgattaag aaaattttaa gatgaatatt
     1681 ttatcctgtt tgaaaggctt tattacaacc gttgattgca aaagcttttc aaaggcagca
     1741 caaaaattgt acatatcccc ctctaaattg agcaaacaaa taacctggct ggaggaggaa
     1801 ttaaaagtta ccttattgat acgctctacg acacgattaa ctgtaactga ttcaggtcat
     1861 aggttgtatg aaaagtcatt gcagcttttt gagcaattag aagcagtcaa agtcattgca
     1921 acacctgatc agctcgaatt aaatggcgtc atcaggctct atcttactgt aactccggca
     1981 attccctatt taaccacatt aagtattcaa tttatgcgac aataccccaa gattgaagta
     2041 gatattattg tcggttcgga tacaaaagaa ctctattcct ctacctttga tctggctatt
     2101 tcatttgatg atgtcaaaca tccaaaactg gtttgcaaaa ggttattttc aattcaaaga
     2161 aaggtctttg cctcacctgg ttatattgct caacatggac ttcctcagac actcgacgaa
     2221 ttaccaaagc ataattgttt gattaatact ctctatggcc tgcaaaataa atgggttttt
     2281 aacaaaaaaa tcattcatgt ctcgggaaat tttaaaagca acaatgctgg tgtcttaaaa
     2341 caggctgcag tagcagacgt ggggattttg tgggcacctt atttttcagt ttatgaagaa
     2401 ataaaaaatg aagcattaat tccagtattg ccccatgaat cttcaccaga tatcaatctt
     2461 tatgcaattt gccccaagcc cactatagat gaacaaaaaa ttaatttact tttgaacttt
     2521 ttttgcgaaa aggccttgtc tgaaaacatt gcgtcatcct ttattgatga aaataattaa
     2581 agatgggtca gtaatttaat ctatttt
//
LOCUS       DAXLDT010000275         2528 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXLDT010000275 DAXLDT010000000
VERSION     DAXLDT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.276"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1335)
                     /locus_tag="ACF2SP_003069"
     CDS             complement(<1..1335)
                     /locus_tag="ACF2SP_003069"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFD2360824.1"
                     /translation="MKRYSKRLLTYIYLLMKSISLAFTMQQLSYSTDKIPIPINKLPD
                     DLNKVMNKSIYKKALWGLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGELLNERGA
                     DYQSVTTVHYDGVVHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGN
                     AIITQTNLLAGYQNLAKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDV
                     VDIVILPSLINENANVSWQPHSSAFTVINQLLTVNTHEKYNLELESKLPACIGKPGCS
                     GIIKGHLPINFIPPLTHNYPLVQTFRITKPSNYARTIFIEALQEAGINVSSIDKTKEN
                     PKKLLKSNNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMI
                     ESLNVEKKVLDKKYGISPSEYHFIDGSGGGETKATNTAITKWLTIMSKRPVFN"
     gene            1474..>2528
                     /locus_tag="ACF2SP_003070"
     CDS             1474..>2528
                     /locus_tag="ACF2SP_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2360825.1"
                     /translation="MSKVIHKLSLFLFKKFKIIWILLTIGILISAFYANWNINQKRAY
                     KDLKEVAINLSNNIDGFIEDLLQEIYALPVYGKKIVDCKSDLYPFLEHITLNNPKISG
                     VIISDNKHNLFCSTLPNNKDLIATPIRSRSIIGPYNLPLFDQPVYLIQQKMGNYLIGI
                     LVIASVLKSELQTDKNQSTSIALYNEYEKKNIFRIRYTEQNKNWVLSNTQETDTQYTS
                     LGLVAIEKLQSINGVSVVVSENNKTIRHNFWYSQTITILIVLICSFILYALIKNMMTK
                     RYSLQGAIKLAIKNEEFYPVYQPLFDCDEERFTGVEVLLRWENEDSQIIMPDFFIAEA
                     EATGLIVPITLQIIEIA"
BASE COUNT          809 a          433 c          421 g          865 t
ORIGIN      
        1 gttaaatact ggtcttttag acattatagt aagccatttt gttatagcgg tattggttgc
       61 tttcgtttcg ccgccgccgc tgccatcaat aaaatgatat tctgatgggg atatgccata
      121 ttttttatcc aggacttttt tttccacgtt tagactttca atcatggagc gcgcgccttt
      181 tgttagtccg tacaacacca ggctggtatc tgctccgata ttataactaa ctttgagaat
      241 atattttaca tgctctccca atggcaatga tttcaaagca gcaattttgt tattcttatt
      301 gtacaagtta ttagatttta gtagtttttt cggattttct ttggttttat caatcgatga
      361 aacattgatg cctgcttctt gtagtgcctc gataaaaatt gttctggcat aattagatgg
      421 tttagtgatt cgaaaagttt ggactaatgg gtagttgtga gtaagtggcg gtataaaatt
      481 gataggcaaa tggcctttaa tgatgccaga acagcctggt ttacctatgc aggcaggtaa
      541 ttttgactca agttcaagat tatatttttc atgcgtatta accgtaagaa gttggttgat
      601 aacggtgaac gccgatgaat gtggctgcca cgatacattt gcattctcgt ttatcaaact
      661 tggaagaatt acaatatcaa ctacatcatc gtttataaaa ataggtgtta catcaaattc
      721 acccctgaaa ttaaatggtt caaacaatct atcgtctatc acaatgtcgc ctgaaataga
      781 acgaatgcca gctttgtaaa cttgtttagc gagattttga taacctgcta aaagattagt
      841 ctgtgtaata attgcattac ccaatgaatt tgcctcgtta tgatcgtatt cagtgatagc
      901 gacatgtcca tcaggtaaag ttcgtccacc catggtcaaa tcacctgaag caacgagtac
      961 cagattccca ttcagattac cattgtgtac tacaccatca taatggactg tagtaacaga
     1021 ttgataatca gctcctcttt cattcaatag ttcgcctaca ctaaatatct tgcgcacaga
     1081 accaatgtaa aactgcgtac ttgagttgag attcagcaat acattaccag tatcaagatc
     1141 aacaacacgt aacccccaca atgctttttt ataaatagat ttattcatca ctttgtttag
     1201 gtcatcaggt agtttattga ttggtatagg gattttatct gtgctgtatg atagttgttg
     1261 catcgtgaac gctagagata tgcttttcat aagtagataa atataagtca gtaatctttt
     1321 cgagtatctc ttcatcattc gcatttccag taatatatgt ttttattctt ttaggtttgt
     1381 ccaatttgtc ataataactg ttaaaaatta ttgggaaatt agcagaaatc acattaaact
     1441 ctatagaaga agcctataac aaagctcaag gatatgagta aggttattca caaattatct
     1501 ttatttcttt ttaaaaaatt taaaatcata tggattctct taactattgg tatcctgatt
     1561 tcagcttttt acgctaattg gaatattaac caaaaaagag cttataaaga tttaaaagaa
     1621 gttgcaatca atctcagtaa caacatagat ggttttatcg aagatctact tcaggaaatc
     1681 tatgctttgc ctgtatatgg gaaaaaaatt gtcgattgta aatcagattt atatccattc
     1741 ttggaacata tcacactgaa taatcctaaa atctctggtg ttattattag tgacaacaag
     1801 cacaatctat tttgttcaac tttacctaat aataaagatc ttattgcaac accgattcgt
     1861 tcacgttcta ttataggccc ctataattta ccattatttg accagcccgt ttatctgatc
     1921 caacaaaaaa tgggcaatta ccttataggt attttagtta ttgcttctgt cttaaaaagt
     1981 gaattacaaa cagataaaaa tcaatccacc tctatagccc tatataatga atatgagaaa
     2041 aaaaatatct ttcgtattcg atatactgag caaaataaaa actgggtatt aagcaatacg
     2101 caagaaactg acacacaata tacctcatta ggtctcgttg ctatagaaaa actacaaagc
     2161 ataaatggtg tttctgttgt tgtttctgaa aacaataaaa ccatccgtca taacttttgg
     2221 tatagtcaaa caataaccat tcttattgtt ttgatttgtt cctttatttt gtatgccctt
     2281 ataaaaaata tgatgaccaa acgttattca ttgcaaggcg caataaaact agctattaaa
     2341 aatgaggaat tttatccggt atatcaacct ttgtttgatt gtgatgaaga acgattcaca
     2401 ggggttgaag tgttattgcg atgggaaaat gaagacagtc aaatcattat gcctgatttt
     2461 tttattgctg aagcagaagc taccgggtta attgtgccaa ttacactgca aatcatagaa
     2521 attgcctt
//
LOCUS       DAXLDT010000276         2527 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXLDT010000276 DAXLDT010000000
VERSION     DAXLDT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.277"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1694
                     /locus_tag="ACF2SP_003071"
     rRNA            <1..1694
                     /locus_tag="ACF2SP_003071"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1818..1933
                     /gene="rrf"
                     /locus_tag="ACF2SP_003072"
     rRNA            1818..1933
                     /gene="rrf"
                     /locus_tag="ACF2SP_003072"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2040..2115
                     /locus_tag="ACF2SP_003073"
     tRNA            2040..2115
                     /locus_tag="ACF2SP_003073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Thr,seq:tgt)
     gene            2188..2272
                     /locus_tag="ACF2SP_003074"
     tRNA            2188..2272
                     /locus_tag="ACF2SP_003074"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Tyr,seq:gta)
     gene            2293..2366
                     /locus_tag="ACF2SP_003075"
     tRNA            2293..2366
                     /locus_tag="ACF2SP_003075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2325..2327,aa:Gly,seq:tcc)
     gene            2419..2494
                     /locus_tag="ACF2SP_003076"
     tRNA            2419..2494
                     /locus_tag="ACF2SP_003076"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2452..2454,aa:Thr,seq:ggt)
BASE COUNT          667 a          524 c          721 g          615 t
ORIGIN      
        1 tctgtaggct gatgaaggtg cattgagaag tgtgctggag gtatcagaag tgcgaatgct
       61 gacatgagta acgataatgt gggtgaaaag cccacacgcc ggaagtccca ggtttcctgc
      121 acgacgttaa tcggagcagg gtgagtcggc ccctaaggcg aggctgaaga gcgtagtcga
      181 tgggaaccag gttaatattc ctggactttt tataagtggt gaagtgggga cgaagaaggc
      241 taggtgagcc aggcgttggt tgtcctggta cttgcatgta ggggggaaga cttggcaaat
      301 ccggttttcc ataactctga ggtgcgaagt ggttctgacc ttttggtaca gagaagtcat
      361 tgatgcccgg cttccaggaa aagctgctag ccataactta tagagaaccg taccgcaaac
      421 cgacacaggt ggacaggtag agaatactaa ggcgcttgag agaactcggg tgaaggaact
      481 aggcaaaatg gtaccgtaac ttcgggagaa ggtacgccct tttgggtgag ggcatttact
      541 gtctgagctt aagagggccg cagagaccag gtggctgcga ctgtttatta aaaacacagc
      601 actctgcaaa ttcgtaagaa gacgtatagg gtgtgacgcc tgcccggtgc cggaaggtta
      661 attgatgggg ttatcttcgg agaagctctt gatcgaagcc ccggtaaacg gcggccgtaa
      721 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg
      781 cgtaacgatg gccacactgt ctccacccga gactcagtga aattgaaatc gctgtgaaga
      841 tgcagtgtac ccgcggctag acggaaagac cccgtgaacc tttactatag ttttgcactg
      901 gactttgatg atgactgtgt aggataggtg ggaggctgtg aagtgaggac gctagttctc
      961 atggagccgc ccttgaaata ccaccctgtt gttattgagg ttctaacttg gtccagtaat
     1021 cctggatgag gacagtgtat gatgggtagt ttgactgggg cggtctcctc ccaaagagta
     1081 acggaggagc acaaaggtac cctcggtacg gtcggacatc gtaccaagag tgtaaaggca
     1141 aaagggtgct tgactgcgag agtgacggct cgagcaggaa cgaaagttgg tcttagtgat
     1201 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
     1261 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
     1321 tcacatcctg gggctgaagc aggtcccaag ggtatggctg ttcgccattt aaagtggtac
     1381 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgtaggaa
     1441 aattgagagg agctgctcct agtacgagag gaccggagtg gacgaacctc tggtgcaccg
     1501 gttgtcacgc cagtggcatg gccgggtagc taagttcgga cgggataacc gctgaaagca
     1561 tctaagcggg aagcctccct caagatgagt tttcccatga agcccgttga agactacgac
     1621 gttgataggc aaggtgtgga agcgcagtaa tgcgtgaagc taacttgtac taattggctg
     1681 attgtcttga ccatataatc tgagtgactt cagattatga tattgatttg tatacgtgaa
     1741 acgtatcgtg taaactctga ctctttacca aacctgtggc ttaacatagc aatcaaagcc
     1801 tcaggtaatc cagttttcct ggcgactata gcgatttgga accacctgat accatctcga
     1861 actcagaagt gaaacatttc cgcgccaatg atagtgtgag gcttcctcat gcgaaagtag
     1921 gtcatcgcca gggttttatt ttatagtgcg gcatgtaaag ccgactaaat taaaataggt
     1981 tagctgtatt cgcataatat aaattaaata agcgctaacc tatcatatgt aaagaatatg
     2041 ctggcgtagc tcagttggta gagcaactga cttgtaatca gtaggtcccg ggttcgattc
     2101 ctggtgccag caccatctaa atagggctaa acgttgacta aaaatgttga caatccattc
     2161 atcttaaaag acaatagcgt tctttttgga ggggttcccg agcggtcaaa gggatcagac
     2221 tgtaaatctg acggctcagc cttcgaaggt tcgaatcctt ccccctccac cagttaagag
     2281 agaagttaaa aagcgggtgt agttcaatgg tagaacttca gccttccaag ctgatagcgt
     2341 gggttcgatt cccatcaccc gctccaaagt agtgaatagt atttgcttgt tggtgtgagt
     2401 tagcaagctg atatgaaagc ccacatagct caggcggtag agcacttcct tggtaaggaa
     2461 gaggtcgttg gttcgagtcc agttgtgggc accatgtaat ataaattagc aaacggggag
     2521 actttcc
//
LOCUS       DAXLDT010000277         2508 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXLDT010000277 DAXLDT010000000
VERSION     DAXLDT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.278"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..220
                     /locus_tag="ACF2SP_003077"
     CDS             <1..220
                     /locus_tag="ACF2SP_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2360826.1"
                     /translation="HEIANLVHYYRKQSGLSQQELAHLAGVGKTVIYDIEKGKESVRL
                     NTLLKVLDVLNIQIKFETPFPQTTDNNS"
     gene            217..453
                     /locus_tag="ACF2SP_003078"
     CDS             217..453
                     /locus_tag="ACF2SP_003078"
                     /inference="COORDINATES: protein motif:HMM:TIGR03071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360827.1"
                     /translation="MRKAYVSVNGIKAGILEELQSGKYQFSYIDDYHESVQGQILPFA
                     SILKNYKSYKSQWEQRARSDPSPNPNTILSPNIP"
     gene            762..1460
                     /locus_tag="ACF2SP_003079"
     CDS             762..1460
                     /locus_tag="ACF2SP_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360828.1"
                     /translation="MKFFNPNDKQESTFDKCMAEYRNLVFGNISERVNQLNERAPNFK
                     PIDFEREQSVQVGVGECAFINADTEERLILGSVGAGTCFILAIVDPNNNSAWLAHVDV
                     LVSMPQLKEMLTKVFTKINPNSAVAHIVGGNDSSIVQGIAICNELEKNGVTISSALLR
                     SKMEMDQLSLAIDPKTGTIYAPEANNLKARNDLWRNLRNRMTKSNEVGSIGLTEISPL
                     PIESTSKHYSAPPI"
     gene            1457..1675
                     /locus_tag="ACF2SP_003080"
     CDS             1457..1675
                     /locus_tag="ACF2SP_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFD2360829.1"
                     /translation="MRQWWSKRKRSGSDLAFCFFILRSKKQELTIVPQDILNYITMFY
                     NSHRLHSYLGYLSPNQFEKQSNLLMKVA"
     gene            complement(1751..1978)
                     /locus_tag="ACF2SP_003081"
     CDS             complement(1751..1978)
                     /locus_tag="ACF2SP_003081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360830.1"
                     /translation="MSDSAGKLKTPKFKKLRGKPGEYLKELSALPIKHGLELAIQQIK
                     RSDIVVSRNQGLNYGKLFDDAKRRKKLSTYF"
     gene            complement(2025..2336)
                     /locus_tag="ACF2SP_003082"
     CDS             complement(2025..2336)
                     /locus_tag="ACF2SP_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HFD2360831.1"
                     /translation="MQIIKSSLFTGDFAWQALDIAQISDASIRLHWADQPYIWHKNDG
                     DEVFVVLSGKVIMHYREGDVENQKELNSGDICYLKEGCEHVAYPKGAARILVIEKVGS
                     I"
BASE COUNT          836 a          486 c          422 g          764 t
ORIGIN      
        1 acacgagatt gccaatttag tccattacta ccgtaaacaa agcggcttat cacaacaaga
       61 gttagcccac ttagcgggtg tcggcaagac agttatatat gatattgaaa aagggaaaga
      121 atcggtgcgg ttgaacacct tgctaaaagt attagatgtt ttgaatatcc aaataaaatt
      181 tgaaacgcct tttcctcaaa caacggataa taattcatga gaaaagcata cgtatcagtg
      241 aatggcatca aggctggaat attagaagaa ttacaaagcg gaaaatatca attcagttac
      301 attgatgatt atcacgagag cgttcagggt cagatcttgc cttttgcctc tatactcaag
      361 aattacaaaa gttataaatc acaatgggag caaagggcaa gatctgaccc tagtcccaat
      421 cccaatacga tcttgagtcc aaatattcca taaaaaaacg ttcaagatct gaccctattc
      481 ccatttgctc gcaaacggga ttacagccct gatgaacaca agcctgtttg caagcaaaca
      541 gcctacgcta gcttatactg gaataatgta aaccaaaata atcaccaatt tcttttaagg
      601 taaattgtcc actttagtac gcaacttgaa tactttcatt tcttgagtgg acataggagt
      661 cttttgattg gcagtttttt gtctatctga gttagtagac aaaccctcac ttatgaacta
      721 tataaaccat aagcagtcct aagcttacaa cgaggtaagc aatgaaattt ttcaacccta
      781 atgataagca ggaaagcact tttgataaat gtatggcaga atacagaaat cttgtatttg
      841 gtaatataag tgaacgcgtt aatcaattaa atgaacgtgc accaaacttt aaaccaattg
      901 attttgaaag agaacaatca gtacaagttg gtgtgggcga atgtgcattt ataaacgctg
      961 atacagaaga acgactgatc ttaggctctg taggcgcagg aacttgtttt attctagcga
     1021 tagttgatcc caacaataac tctgcatggc ttgctcatgt agatgtttta gtttctatgc
     1081 cacaactcaa agagatgtta acaaaagtat ttacaaaaat taatcctaat tctgctgtag
     1141 cgcatatagt tggaggaaat gattcatcta tagtacaagg tatagcaatt tgtaatgaat
     1201 tagaaaaaaa tggggtaacg attagctcag cattattaag atcaaaaatg gaaatggatc
     1261 aattaagctt agccatagat cctaaaaccg gcactattta tgccccagaa gctaataatc
     1321 taaaagcacg taatgattta tggcgtaatc tacgcaatag aatgactaaa agtaatgaag
     1381 tgggctcaat cggacttaca gaaatttcac cacttccaat cgaatcaact agcaagcatt
     1441 atagtgcccc acctatttga gacagtggtg gtctaagcgt aagcgttcag ggtcagatct
     1501 tgccttttgc ttttttattt taagaagcaa aaagcaagag ctgaccatag tcccacagga
     1561 cattttgaat tacatcacca tgttctataa cagccataga ttgcattctt atttaggata
     1621 tttaagccca aatcaatttg aaaaacagag caatctttta atgaaagttg cttaactggg
     1681 gtgtaacatt tgggttgacc acgtcacatc gagttgcttc ataataaata ttcccctaaa
     1741 caacatgagg tcagaagtat gttgataatt tttttcttcg cttcgcatca tcaaacaatt
     1801 ttccatagtt cagcccttga ttcctggata caacaatatc agatctttta atttgctgga
     1861 tggctaattc taaaccatgc ttaatcggca gtgcagaaag ctctttgaga tattcccctg
     1921 gcttgcctct taatttttta aatttaggag tttttaactt gcctgcagag tcactcatca
     1981 ataaatatcc ttttttggag attacctgaa aacacaaatc aattttaaat actaccaact
     2041 ttctctatga caagtattct tgcagcccct tttggataag ctacatgctc acatccttct
     2101 ttcaaataac atatatcccc actatttaat tctttttgat tttctacatc accctcacgg
     2161 tagtgcataa tcacttttcc agacagcaca acaaaaacct catccccatc attcttatgc
     2221 caaatatatg gttgatctgc ccaatgaagt ctaatgctcg catcagatat ttgagcaata
     2281 tctaatgctt gccatgcaaa atcgccagta aataatgatg atttaattat ttgcatatta
     2341 ccttacgctt attaaatttt agtggtttta gactcctggc tagcagccag aacgaataat
     2401 cctttttgct ccaatttcct aactaaatta taatcacttt caacttgttc tttcgattca
     2461 tggcttagaa ttaatagacc aggcatggaa aaaagatcga ctgttttc
//
LOCUS       DAXLDT010000278         2495 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXLDT010000278 DAXLDT010000000
VERSION     DAXLDT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.279"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..528
                     /gene="ceg29"
                     /locus_tag="ACF2SP_003083"
     CDS             <1..528
                     /gene="ceg29"
                     /locus_tag="ACF2SP_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector Ceg29"
                     /protein_id="HFD2360832.1"
                     /translation="DPFFLMYLDKLKEDPAHLFRNDKEIIIAAIEKQPFLYKNFSRGY
                     KEDEEVILCALKNNRFIIYDVPRTVREVFKEIADIKEDKARVDACDQLIDKLSPQRVS
                     KIKSLDLFKSQLTLQAQNITKKSGEQVNNRQLQRSTAPITPEGITSLVHLGLFRDTQI
                     QYEEKEESLHNKYRK"
     gene            complement(648..1469)
                     /locus_tag="ACF2SP_003084"
     CDS             complement(648..1469)
                     /locus_tag="ACF2SP_003084"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFD2360833.1"
                     /translation="MIEERIIKPDDCLGLRRGSIYTVEEPQKDLICPHHTINSNLKPY
                     TCIPLFAQSDILGLVCMEWKSFKKEAEEEINKENNQEILASMMGEQIALGISNIMLRE
                     TLRNQSLRDVLTGLYNRRYLNEALDRELNRCARKSLSLAVLMLDIDHFKQFNDKFGHE
                     AGDMVIQAFANVLRQVTRKEDIVCRYGGEEFILIIPEIDLEAALQRAQSIHDAVSRIH
                     LRYGGNALTQITISIGLTMYPAHGNDMQDLITAADNALYEAKNSGRNKTVLYHKN"
     gene            complement(1447..2346)
                     /locus_tag="ACF2SP_003085"
     CDS             complement(1447..2346)
                     /locus_tag="ACF2SP_003085"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFD2360834.1"
                     /translation="MIAKSKTDMNRIQMGTNKEFPGKSHLKPSKSCGTDGSNFQVEFL
                     LDVIQKLSLAKDLETIMFIVRKAARTLTGADGSTFVLRDGNQCYYAEEDAIVPLWKGK
                     RFPMSACISGWVMEHRQSVIIEDIYSDPRIPFDAYRPTFVKSLAMVPIRTAAPLGAIG
                     IYWAEYYFPTEEQLNWLQVLADSTSVAMENVMLQSELEKGQKETTAQIEMTRKLMEVN
                     KNLECALYELNHRNKEMQWLKELSSTLQTCLYIKEAYHLIALYAAKLLPESSGIFYVM
                     HPSRNYLEAMSSWGGLFDRRKDH"
BASE COUNT          785 a          516 c          434 g          760 t
ORIGIN      
        1 gatccttttt ttctaatgta tctggataaa ctcaaagaag atcctgcaca tttatttagg
       61 aatgataaag aaattattat agcagccatc gagaaacaac cctttttgta taaaaatttt
      121 tcccgaggat acaaagaaga cgaagaggtt attctgtgtg ctcttaaaaa taatcgattc
      181 ataatctatg atgtaccaag aacggtgaga gaagtattca aagaaatagc tgatatcaaa
      241 gaagataaag caagagttga cgcgtgtgac cagctaattg acaagctttc accacaacgt
      301 gtttctaaaa tcaaaagcct cgacttgttt aaaagccaat taactctgca agcacaaaat
      361 ataacaaaaa aaagtggtga acaagtaaat aaccgtcagc ttcaaagatc aactgcgcct
      421 ataacacccg aaggtatcac ctctcttgtt catttaggcc tgtttagaga cacacaaatc
      481 caatatgagg aaaaggagga atctctacac aacaaatatc gaaaatgaag cgaataaatg
      541 ttgataaggg ttgctctatc gcaaattcat tacagaaata gccaagcata gccttaatag
      601 attgaataag gccggaagct aatatgtttt gattgcaaca aagtacccta atttttatga
      661 tacaacaccg ttttattgcg tcctgagttt ttggcttcat aaagcgcatt atcggcggcg
      721 gtaataaggt cttgcatatc gttaccgtgc gcggggtaca tagttaatcc tatggaaatt
      781 gtgatttgag ttaaagcatt acctccataa cgcaaatgaa tacgactgac tgcatcatga
      841 atagattgag ccctttgtaa ggctgcttcc agatcaatct ctggtataat caggataaat
      901 tcttcccctc catagcggca gactatgtcc tcctttcggg taacctgacg caaaacatta
      961 gcaaaagcct gtatcaccat atcacccgct tcatgtccga atttatcatt aaactgttta
     1021 aaatgatcaa tatccaacat taacacagca agtgataatg attttcttgc acagcggttt
     1081 aattctcgat ccagcgcttc attcaaataa cgtcgattat aaagccctgt gagaacatct
     1141 cgtaaagatt ggttacgcaa agtttcacgc agcataatat tggaaatacc aagcgctatt
     1201 tgctctccca tcatacttgc gagaatttcc tgattatttt ctttattgat ttcttcctct
     1261 gcttcctttt taaaagattt ccattccata caaaccaaac cgagaatatc gctctgagca
     1321 aataaaggaa tacaggtata aggctttaaa ttactgttga ttgtgtgatg ggggcatatt
     1381 aagtcctttt gaggctcttc tactgtataa atcgagccac gacgcaaacc caggcaatca
     1441 tctggcttaa tgatcctttc ttctatcaaa gagccccccc catgagctca tagcctcaag
     1501 ataattacgt gaaggatgca tgacatagaa aattcctgat gactcaggta ataatttagc
     1561 tgcatacaga gcaattaaat gataggcctc cttaatatat aaacaggttt gcaatgttga
     1621 acttaactcc ttcaaccatt gcatttcttt attgcggtgg tttaattcat ataaggcaca
     1681 ttcaagattt ttattgacct ccattaattt tctagtcatt tctatttgag cagttgtttc
     1741 tttctggcct ttctccaatt cggattgcag catcacattt tccattgcaa cagatgtgct
     1801 gtcagccagt acctgcaacc aatttaattg ttcctctgta ggaaagtaat attctgccca
     1861 ataaattcca atggcgccca aaggggctgc tgttctgata ggaaccatcg ccaagctctt
     1921 tacaaaagtc ggccgataag catcaaaggg aattcttgga tcagaataaa tatcttcaat
     1981 aatgaccgac tgtcgatgtt ccataaccca accgctgata caagcactca tagggaatcg
     2041 tttgcctttc caaagaggga caattgcatc ttcctccgcg taatagcatt ggttgccatc
     2101 acgcaataca aaggtcgaac cgtcagctcc agtcaatgta cgagcggctt tacgcacaat
     2161 gaacatgatt gtttccagat cttttgcaag agaaagtttt tgtatgacat ccaataaaaa
     2221 ctcaacctga aaattggagc catcagttcc acaagatttt gacggtttga ggtgtgattt
     2281 gcctgggaac tctttatttg ttcccatctg tattctattc atgtccgttt tactctttgc
     2341 tatcatccct gtctctttgt aattataata ctaaatgtgt gttttttaaa caaaatatcc
     2401 ttcggatcac tcctctctat aagttgtata gtaacttaaa ttttcatttc gaataattgt
     2461 tgaaacaagt tgacgtctgc tcaaagacca tttat
//
LOCUS       DAXLDT010000279         2472 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXLDT010000279 DAXLDT010000000
VERSION     DAXLDT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.280"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(1..1104)
                     /locus_tag="ACF2SP_003086"
     CDS             complement(1..1104)
                     /locus_tag="ACF2SP_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360835.1"
                     /translation="MRQELLKYLEMYHPQIAPFMHLQINEWGKNRPLEGVKILHHVPL
                     VPNTLLKIAALVAANAEVTVTNPASFCQAHPDAITCLHKSGIPYVENLDLLRDETFDV
                     YLDCGGQLHQSLGRPLRGAIELTGSGDQYYRSLKTLDFPVISIDHTMTKQLETVFGCA
                     ESSHQAIQKLTGVDPTNKNWLIFGFGKIGRGLAYFCLEHQVPVTVVDSSVHQCDLASN
                     LGIEAIRPEEHDKLEKAVAKADIILTATGGKNIMSKYPRSWFDGKILGNLGLHDEFGP
                     NFTSQDILYDKKPINFCLYDPTPMIFIDPEFYLHNLGCLLLLQGNFPRGMHDIPEDID
                     SSIIKDWCQYHSRSEKSIRRWFLTSHDFSLKTN"
     gene            1482..1949
                     /locus_tag="ACF2SP_003087"
     CDS             1482..1949
                     /locus_tag="ACF2SP_003087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360836.1"
                     /translation="MSEQQSSSLAIEQQDKLTRNIIHDAALASNNISSDSFDLEKAKS
                     LCVDGLIALGVQNGLESMLAAQMLSIHKLQQRSMTYANASDNLELKTYYTNSAIKLAN
                     CFVQQANMLAKLRGVGGQKIIVEHVDIHQGGQAVVGNIQGSMGRSNENIKTTS"
     gene            1961..2224
                     /locus_tag="ACF2SP_003088"
     CDS             1961..2224
                     /locus_tag="ACF2SP_003088"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HFD2360837.1"
                     /translation="MYAFDNAPRCGAKAKITNGNPCRCPAIKGKARCRVHGGAEGSGA
                     PQANVNALKHGNTTAATKSFRRDIRQAIRFNKELLSECIKSSY"
     gene            complement(2261..>2472)
                     /locus_tag="ACF2SP_003089"
     CDS             complement(2261..>2472)
                     /locus_tag="ACF2SP_003089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360838.1"
                     /translation="FKRQQKFSSIKNVCEEYWDIIKNYARKQEKINPNLIKILSYWIK
                     CCIFLYILGLSFAWVRQGFKNNKVN"
BASE COUNT          804 a          463 c          450 g          755 t
ORIGIN      
        1 ttaatttgtt ttaagagaaa aatcatggga agtcagaaac caacgtcgaa tacttttttc
       61 agaacgacta tgatattggc accagtcttt aataatagag gaatctatat cttcaggaat
      121 atcatgcatt ccccgaggaa aatttccctg taaaagcaat aaacatccca gattatgcaa
      181 ataaaactca ggatctataa aaatcattgg tgtaggatca tataggcaaa aattaatagg
      241 ttttttatcg tataatatat cctgagaggt gaaattggga ccaaactcat catgcagccc
      301 taaattcccc aagattttgc catcaaacca ggatctgggg tatttgctca tgatattttt
      361 accgcccgtt gcagttaaaa taatatctgc tttagccact gctttttcga gcttatcatg
      421 ctcctcaggt ctaatagcct ctataccaag attagaagct aaatcacatt gatggactga
      481 actatccact acagtaactg gtacttgatg ttccagacaa aaataagcga gccctcgccc
      541 tattttacca aacccaaaga ttagccaatt cttgtttgtg ggatcgacac cagtgagttt
      601 ttgaatcgct tggtgagaac tttcagcaca gccaaatact gtttccaatt gtttcgtcat
      661 tgtgtgatca atgcttataa ccggaaaatc aagtgttttc agactacgat aatactgatc
      721 ccctgagccc gttagttcaa tagcccctct caaaggtctt ccgagactct gatgaagttg
      781 cccaccacaa tccagataaa catcgaacgt ttcatctctt aaaagatcaa gattttcaac
      841 ataaggaatt cctgatttat gaagacaagt aatcgcatca ggatgtgcct gacaaaaaga
      901 agccggattg gttacagtca cctcagcatt tgcagcgaca agagctgcta tttttagcag
      961 tgtattggga accaaaggca catgatgcag tattttcacc ccttccagtg gcctgttttt
     1021 gccccattca ttaatctgaa gatgcataaa tggcgcaatt tgcgggtgat acatttctaa
     1081 atactttaat aactcctggc gcattgttta gaccatatga gtatgagaaa gaatgacaat
     1141 actataataa aactcattag caataacacg aattaataaa gcatatttta atttttgagg
     1201 attaggcgat taataattga ttgggccgta taggattcga acctatgacc ctgagattaa
     1261 gagtactatg ttattaatta atcacagtca atcacaataa ataaaaataa ttaaatcaaa
     1321 tagttagttc ttttattgta ttgccattag ttgccatttt ttcttatttg ttattactga
     1381 gtgtccccaa aatgtcccaa gaagacgggg gctaactatt tcatgaatta ttaaattgct
     1441 gcgataatta aggttattca atttaataat tggtgatact tatgtcagag caacaaagta
     1501 gtagtcttgc tatcgaacag caagataaac tcactcgaaa tattatccat gatgccgctt
     1561 tggccagcaa taatatcagc agtgacagtt ttgatcttga aaaggcgaag tctctttgtg
     1621 tggatggtct tattgccctt ggtgttcaga atgggcttga atcaatgtta gcagctcaga
     1681 tgttgtctat tcataagtta caacaaagat ctatgaccta tgcaaatgcc agtgacaacc
     1741 tggagttgaa gacctactac accaattcag caataaaatt agcaaattgc tttgtccaac
     1801 aggcaaatat gctcgctaaa ttaaggggtg ttgggggaca aaaaattatt gttgagcacg
     1861 tagatattca tcaaggtggt caggcagttg ttgggaatat tcaaggcagt atggggcgtt
     1921 caaacgaaaa tattaaaaca acctcataga ccgagaaaat atgtatgctt ttgataatgc
     1981 gcctagatgc ggagcgaaag ctaaaataac taatggtaat ccttgtcgtt gccccgcgat
     2041 caaaggtaag gctcgttgtc gagtgcatgg aggagctgag ggatcggggg ctccacaagc
     2101 taatgtgaat gcccttaagc atggtaacac tacggcagcg accaaatctt tcagaagaga
     2161 tattaggcag gccattcgct ttaataaaga acttcttagc gaatgtatta agtcaagtta
     2221 ttaactcctg ctttcctttc tcatgcgcat ttgtaataca ttaatttact ttattatttt
     2281 taaatccctg gcgaacccaa gcaaaagata atcccaaaat atataaaaaa atacaacact
     2341 ttatccaata tgataatatt ttaattaaat ttggatttat cttctcctgt ttgcgagcat
     2401 aatttttaat aatatcccaa tattcttcac aaacattttt aattgaacta aacttctgtt
     2461 gccttttaaa ag
//
LOCUS       DAXLDT010000280         2446 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXLDT010000280 DAXLDT010000000
VERSION     DAXLDT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.281"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            227..481
                     /locus_tag="ACF2SP_003090"
     CDS             227..481
                     /locus_tag="ACF2SP_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360839.1"
                     /translation="MIELEPEPDTVLDPKDILTEEMKLEGSDNDMDHNQFTRGDEVDL
                     VGDGVPGYETTGQDGTDDEIEEQREARYTPTLNPNPKNVP"
     gene            577..1329
                     /locus_tag="ACF2SP_003091"
     CDS             577..1329
                     /locus_tag="ACF2SP_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360840.1"
                     /translation="MLVTPRFKIMVVAKKREETQKDERARAIENNPCSICRSLGLPFC
                     RGHGGGSGGGGSDSASDDKVDNNDHQLLAPKNPANVTNIDQLLLHSGLWHLPEDEDFV
                     FEFKDPLALVNMTLNMELGLLMLQGKRELSNEEQNALDELYDTISQEFNLFKNSIKDP
                     QVPLEAMTLAREGNSLTIKIPSPKYYDLFVQQLMEKNLLPGKAPVLQHQNQSVKHPEK
                     SEAAKDSVMEEYKSSLPNPFDISRGPKLPDWI"
     gene            1632..2009
                     /locus_tag="ACF2SP_003092"
     CDS             1632..2009
                     /locus_tag="ACF2SP_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFD2360841.1"
                     /translation="MTTPEITLSRVYSPPEHKEGLWILVDRLWPRGIKKDSIPLDMWL
                     KDIAPSPALRKWFHQDSDHWHEFAKQYAQELKEKPELIEHILALASTSPVTLFYAAKD
                     LQHNHALVLQKVLKSWPKIPEIK"
     gene            2169..2245
                     /locus_tag="ACF2SP_003093"
     tRNA            2169..2245
                     /locus_tag="ACF2SP_003093"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2203..2205,aa:Pro,seq:ggg)
BASE COUNT          791 a          466 c          501 g          688 t
ORIGIN      
        1 ttatattttt tgaaaaaagg tctctttgct tatagggcaa tgagacttta tgtccaactg
       61 aagtagcagt ttagcctgtc attaactcca tatcacccga tggttttttg agtcttttcc
      121 cagcaagggg cagaaggtat tcggtaaact aatccttgct aaatttctta tctttctatc
      181 cctgctacac ttaaatatag agaagattat atttaagggc agcaatatga ttgagttaga
      241 gccagaacca gacacagtcc tggatcctaa agatattttg actgaggaaa tgaagttgga
      301 agggtcagat aatgatatgg atcataatca attcacccgt ggtgatgaag tggatttagt
      361 cggtgatggt gttcccggat atgaaacaac aggacaagat ggaacagatg atgaaattga
      421 agagcaaaga gaagctcgct atactcctac cttgaaccct aatcccaaaa atgtgccttg
      481 atctccgtaa tgaggttcaa ggtgtagttt attgtaaatt ttttgcaaaa tttaccaaat
      541 tgccaaatga aacattcatt ttaaattatc atctgcctgt tagtaactcc aaggtttaaa
      601 ataatggtag tggcaaagaa aagagaagaa acgcaaaaag atgaacgtgc acgtgcaatt
      661 gaaaataatc cctgcagtat ctgtcgttca ttaggattac ctttttgtcg tggtcatggc
      721 ggcggttctg gaggcggcgg atcggatagc gcttctgatg acaaagtgga taataatgat
      781 catcaattgc tcgcaccaaa aaatcctgcc aatgtcacaa acatagatca actactattg
      841 catagcggtc tatggcattt gccagaggat gaagattttg tttttgaatt taaagacccc
      901 cttgctttag tcaacatgac tctgaacatg gagcttggtt tattaatgtt gcaaggcaag
      961 cgcgagctta gcaatgaaga gcagaatgct ctggatgagt tgtatgatac gatttcacag
     1021 gaatttaacc tttttaaaaa ttcaataaaa gacccacagg ttcctctgga agcaatgaca
     1081 ctcgctagag agggtaatag tttgactata aagataccca gccctaaata ttatgatttg
     1141 tttgtgcaac agttgatgga aaaaaatttg ctacccggca aagcaccagt tttgcaacat
     1201 caaaaccaat cagtgaaaca tccggaaaaa tcggaagcgg cgaaggattc agtaatggaa
     1261 gaatataaat catccttgcc taatccattt gatattagcc gcggccctaa attacctgat
     1321 tggatataat ctttcccaga taaggatgag cggaaaaatt tctccatcat gctattctta
     1381 attaaagtga gttatggata aggattatat aagaagcagt cgatatccag gagccgttca
     1441 ggctgaggga tgcataagca tcgtctcgaa gccttagcac gcaacattaa attccataca
     1501 aggcttcgag acagcataaa tgcctcatca gcccgaacga ttggagataa cgcctatctt
     1561 atccataact catgttaatt aaagagatca gcatgatcct gaaatttttc caacgccagg
     1621 aatgacatgg tatgacaacg cccgaaatta ccttatccag agtgtactct ccaccggagc
     1681 ataaagaagg gctatggata cttgtagacc gattatggcc tcggggaata aaaaaagaca
     1741 gtattccgtt ggatatgtgg ctgaaagaca tagctccaag cccggctttg aggaaatggt
     1801 ttcatcaaga cagtgatcat tggcatgagt ttgcaaagca gtatgcccaa gagttaaaag
     1861 agaaacccga attgatagaa cacattttgg cgttagcgag cacttcacca gtcactttat
     1921 tttatgcggc aaaagatctt caacataatc atgcactggt acttcagaaa gttctaaaat
     1981 cgtggccaaa aataccagaa atcaagtagg gcctatttga gcgaaataaa actcagacaa
     2041 gctagtaaac ctaaacactt cagtcattta gttccctcat taaaaatatt ttttgccttg
     2101 accttggagt ttttcgtaag tcctgccctt gttattttag ccttatttag atatcatgcc
     2161 taaccaatcg gggcgtagcg cagcctggta gcgcactagc atggggtgct agtggtcgaa
     2221 ggttcaaatc cttccgtccc gaccatcaaa tcccctcatt ctttgttaag aacctgcttt
     2281 ttggggtaga gcccaattct attgggttaa caataatagt caatttacag ctaaatttat
     2341 ccagggaaat cacatcgttt acaaagcgag cttgggtcat tttgacacaa taatacaatc
     2401 aatctaagat ttattaatta aaataagtag ttcaggatct aataaa
//
LOCUS       DAXLDT010000281         2422 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXLDT010000281 DAXLDT010000000
VERSION     DAXLDT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.282"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..335)
                     /locus_tag="ACF2SP_003094"
     CDS             complement(<1..335)
                     /locus_tag="ACF2SP_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360842.1"
                     /translation="MQYQPIMKQIRAAISKNDADQLLNITSADWVMVYLYTDHIFLNS
                     FTVEQENFDLYDSDEILSLDFPLKESSISCFKHSEDDGYSSSMEVSHEFGLDNSLMWP
                     LFFSRANYK"
     gene            complement(365..856)
                     /locus_tag="ACF2SP_003095"
     CDS             complement(365..856)
                     /locus_tag="ACF2SP_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease superfamily protein"
                     /protein_id="HFD2360843.1"
                     /translation="MKQGGSYANSSGKVLEGLVEFALTKKGFTVTRYKDWKLNPSNFG
                     EELLLKNVPYEGIYKHASTTEFVLISKAYNLNTRIECKWQQVSGSADEKLPYLFLNCS
                     EKMFEPHIIILLDGGGSKTGAVNWLREECDKFNLSQSNASKRQIDLMDMTDFVKWVNT
                     VFK"
     gene            complement(867..1703)
                     /locus_tag="ACF2SP_003096"
     CDS             complement(867..1703)
                     /locus_tag="ACF2SP_003096"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFD2360844.1"
                     /translation="MIKNSEKESIIRIRPFLKWAGGKFQIINKIRASLPKGARLIEPF
                     VGSGAVFLNTNYNKFILADINADLINLFEHLKFEKHDFIHFCKQFFTAEFNKKSTFLS
                     LRTEFNSTTDSHLKAALFIYLNRHAFNGLMRYNSSGMFNTAFGDYKKPYFPENEMLVF
                     IQKAEKAEFKCADYSVIMGEAIKGDVVYCDPPYVPLSASANFTKYHSTAFGQEDQRRL
                     VELAKELAAKGIPVILSNHDTAFTQNLYQGSSIISFEVQRNISCNGSTRGRAREMLAL
                     FN"
BASE COUNT          778 a          467 c          371 g          806 t
ORIGIN      
        1 tctttatagt ttgcacgact gaagaaaagt ggccacatta agctattatc taaaccaaac
       61 tcgtgtgaaa cttccattga actactataa ccatcatctt cgctatgctt gaagcaagaa
      121 atcgaagact cttttaaagg aaaatctaag gacaaaatct catctgaatc atataaatca
      181 aaattttctt gctcaacggt aaatgagttt aggaaaatat gatctgtata taaataaacc
      241 attacccaat ctgctgaagt aatattcaat aattgatcag catcattttt ggagatggcc
      301 gctcttattt gcttcataat tggctgatat tgcatggaaa ctctcttatt caatattctc
      361 atctctattt aaaaactgta ttaacccatt taacaaaatc ggtcatatcc attaagtcta
      421 tttgccgttt tgaagcattt gactggctta aattaaattt atcacactcc tcacgtagcc
      481 aatttactgc tccagtcttt gaaccacccc cgtcaagtaa aataataata tggggctcaa
      541 acattttttc agaacaattc aaaaacaaat aaggtagttt ttcatcagca ctcccagaaa
      601 cttgttgcca tttacattca attcttgtat ttaagttata tgccttagat attaagacaa
      661 actcggtggt actggcatgt ttataaattc cttcatatgg cacatttttt aataatagct
      721 cttcgccaaa attcgaaggg tttaatttcc aatccttata acgagttaca gtaaagccct
      781 tttttgttag agcgaactct actaatccct caagaacctt tccgctacta tttgcatatg
      841 aaccaccctg cttcatggca acacccctaa ttaaataggg caagcatttc acgtgctcgt
      901 ccccttgtgc tcccgttaca actgatattc cgttgcacct caaaactgat aatgcttgat
      961 ccttgatata aattctgagt aaaagccgta tcatgattag ataaaattac aggaatcccc
     1021 ttcgcagcca gttctttagc taactcaact aatcgtctct ggtcttcttg tccaaaagct
     1081 gtggaatgat attttgtaaa attggctgat gcagataatg gaacatatgg ggggtcgcaa
     1141 taaactacat ctcctttaat agcttctccc ataattacgc tataatcagc acatttaaat
     1201 tctgcttttt ccgctttttg aataaaaacc aacatttcat tttctggaaa ataaggtttt
     1261 ttataatccc caaaagccgt gttaaacatg ccagaactgt tgtacctcat gaggccatta
     1321 aaagcatgac gattgagata aataaatagt gcagccttca agtggctatc tgtagttgaa
     1381 ttaaattctg tacgcaatga taggaaggtt gattttttat taaattcagc agtaaaaaat
     1441 tgcttacaaa aatggataaa atcatgcttt tcaaacttca aatgttcaaa caagttaatt
     1501 agatcagcat taatatcagc gagtataaac ttgttatagt tcgtatttaa aaaaactgct
     1561 cctgaaccta caaacggttc aatcaaacga gcccctttag gtaggctcgc acgaatttta
     1621 tttattatct ggaacttacc gccggcccat tttaaaaatg gtctaatgcg gattattgat
     1681 tccttttcac tattttttat cattatttat cctttttatc taattttagt ctagtttaga
     1741 aaattaggac tcatttatgc tctataatta aacagtggcg ctccgcctta tgttatttca
     1801 ctttgagaaa attgctattg gaacagggca ataaagctca aaacctagga tttactaggg
     1861 aagtaactga aggagtactt gatgctacat agcaaaaaaa ccgtttttgt aaggcaacat
     1921 tggagaaagc tatacagcga tcccatttat gttcggtctt actggcggtc acctcctagt
     1981 aggtaaactc tactaaaggc atttgcgcca cccatttcga ttcgctaaaa gtaaaataat
     2041 gaggcaaaaa atcaaacaaa taagtttgtt tttgcttaca gacaaaaatg cagtacccca
     2101 taattgcccc tgaatatata atacaaactc aaatattgag ttttatcata tatatatgaa
     2161 ctcaattctt ctgattaata ttcaaaaggt tcgcggttcg attccgcgca gcggcaccag
     2221 gtataccaat ggttataggt tgttttcaaa atcaccatcc ggattatggg acacatatgg
     2281 gacagttgta gataactatt tacctttagt aactagctta atagctttcc attgtattgc
     2341 ttcatttgta ttgaaaataa atagttctct ttctctgctt gggtcatttg tatacgataa
     2401 ttgttttatt cttttttcaa aa
//
LOCUS       DAXLDT010000282         2407 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXLDT010000282 DAXLDT010000000
VERSION     DAXLDT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.283"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..846
                     /locus_tag="ACF2SP_003097"
     CDS             <1..846
                     /locus_tag="ACF2SP_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HFD2360845.1"
                     /translation="KGLGTEKEAEALFALQNYSKIPDIQNNPQAATVTLSKIINSYRV
                     KNDSRTYFFAILEAAQGKELFKIMTYELLQHKDINTLKDVFYRIGQQTGELHKTIIGS
                     DQFITPRDLVTVIHDDFHPENTFYNLETNIFSLIDNESMGDSITEPLPIIRELYGFYE
                     VPIIRWPSEKITVDLLKDADPHDIASIYYDFVLGMASVYQDPQDAKRVLIEAIIDLNN
                     MAIAFLKDRYSEPWMLNPSDKHEAHIVWGKLAKVDTNPQLAELYITKLTIINNVLQSL
                     SGFKR"
     gene            complement(1750..>2407)
                     /locus_tag="ACF2SP_003098"
     CDS             complement(1750..>2407)
                     /locus_tag="ACF2SP_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360846.1"
                     /translation="HEITSGADSGPLLTKIYGQDVRFIYAPDSEKFNLVLNEARKRSY
                     IQPIINLYEPDNIKYLLSRLSDGDSILINGQGDIHKQLIAGRDAEELVDILENDLELK
                     DISLKNLDIDSCMMGRVESYRHKLKRHLKNFQTITTYTDLCTASQSGGVPYRMWIEER
                     VDMDVFYTESDLNIKATRIIEYTDTYKNSLKEIWKTNPYNLEEIDLSDHIDILVIASC
                     "
BASE COUNT          791 a          408 c          390 g          818 t
ORIGIN      
        1 aaaggtctgg gaacagaaaa agaagcagag gcattgtttg ctttacaaaa ttactcaaaa
       61 attcctgata ttcaaaataa tccacaagcc gcgactgtta cattaagtaa aatcattaat
      121 tcatatagag tgaagaatga ttcgaggact tatttctttg caattttaga agcggctcaa
      181 ggaaaagagt tatttaagat aatgacttat gaattgctac agcataaaga tattaacacg
      241 ttaaaagatg ttttttatcg aatagggcaa cagacgggtg aattacataa aacaattatc
      301 gggagtgatc aatttatcac tccaagagat ttagtgaccg ttattcatga tgattttcat
      361 cctgaaaata cgttttataa tcttgaaaca aatatcttct ctttaattga taatgaaagc
      421 atgggtgata gtattacaga gcccttgccc attatcagag aattatatgg gttttatgaa
      481 gttcctatta ttcgctggcc ctcagaaaaa ataactgttg atctcttaaa ggatgctgat
      541 cctcatgata tagccagtat ctattacgac tttgttctcg gtatggcctc ggtgtatcaa
      601 gacccccaag atgcaaaacg agtgttaatt gaagctatta ttgatttaaa taatatggct
      661 attgcctttc tgaaggatag atattctgag ccatggatgc ttaacccaag cgataaacat
      721 gaagcacata ttgtgtgggg caagttagca aaagtcgaca ctaatccgca gctagcagaa
      781 ctctatatca ctaaattaac cattattaat aatgtattgc aatcactttc aggctttaaa
      841 cgctagcaga gggtgagtac tctggttatt ccgcaccctc ggattttagc tgcttcagca
      901 gtctttcatt atttgtttaa atgtaataat ggggctgaat tttaattact ctatatactg
      961 atgtagtgtg agcttatttt agattattta cttttgattt tcagaagctg cataaacccc
     1021 aatacatcat ctacttatct atttatttaa agtgtatcgc tgctttatat ctccttgctc
     1081 attgataaaa gacaccttgg taaaggatga atcatctgac taacttctaa attccacaag
     1141 aaatttgaca aactataaac agacttatcc acagaaaatg tggataactt aaataaaatt
     1201 acaggtatta aaaaatgata tcaaccaagc ccaataagga ttgttatagt ttactgatta
     1261 atcggttgtg atttggtaaa ataagatatc cacagacaac ggataaggga aaatattttt
     1321 gacctgcata tcttatacaa ataaaaaata aaaaaacagt cttgactcga actattttta
     1381 ctatttattt tgacccactg cttgattaaa cttttttctt aaacaataac tctcctgcct
     1441 tagtgaggtt tttaaatttg gaactatagc catcttgtct ggaacaacta aaatagcgag
     1501 caagcctatg cctgataaat aggcaaacta atattatgtg gagatggttt gttcatgtgt
     1561 tttaaaattg gcaacgtatc cattcttctt tggatttcaa atctctaata attttggtat
     1621 ggttgcttaa tgttggtgtc cggcttttcg aggaaggatc agtctgagat gttatgactt
     1681 aacgcatcac acagtttaaa gattgaaaaa tttgcgagtt acccaactca attcaagcta
     1741 ttgtcgaaat tagcatgatg caattactaa aatatcaata tgatcagaca gatcaatttc
     1801 ttccagatta tacgggtttg ttttccaaat ttcttttaat gaatttttat aggtatccgt
     1861 atattcaata atgcgggtcg cctttatatt tagatcgctt tcagtataaa aaacatccat
     1921 atccactctt tcttctatcc acattctgta aggcactcca ccactttgtg atgctgtgca
     1981 taaatcggta tatgttgtaa tagtttgaaa gttctttaaa tggcgtttta atttatgacg
     2041 ataactttca actcttccca tcatgcaaga atctatatct aaatttttta gtgatatatc
     2101 ttttaattcc aaatcatttt caagaatatc taccaactcc tcagcatcac gtcccgcaat
     2161 taattgctta tgaatatcac cctgcccgtt aataagtata gaatcgccat cacttaatcg
     2221 agataataag tatttaatat tatctggctc ataaagattg attattggtt gaatataact
     2281 tcttttcctg gcttcgttta aaaccagatt aaatttttcc gaatcagggg catagataaa
     2341 tctaacatct tgaccatata tttttgttaa aagtgggcca gaatctgcac ctgaagttat
     2401 ctcatga
//
LOCUS       DAXLDT010000283         2387 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXLDT010000283 DAXLDT010000000
VERSION     DAXLDT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.284"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..358
                     /locus_tag="ACF2SP_003099"
     CDS             <1..358
                     /locus_tag="ACF2SP_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFD2360847.1"
                     /translation="LRDRACFDAANAMSQLVAKLTTYTNYLEKNPDPFLIKRYKDCCI
                     VEMDLANLTLQNHRDALWLVAELSTAILLLGVGYLFAFGINYYYTGRMGLFSQTRSEK
                     LVEEMKSSVLGIAVGN"
     gene            complement(412..1221)
                     /locus_tag="ACF2SP_003100"
     CDS             complement(412..1221)
                     /locus_tag="ACF2SP_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1484 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360848.1"
                     /translation="MPSMEMNCCIGKSLNYMRFSMSSFKHSCEKIDINLAHNVLTICP
                     KNREALQRITALFNNLYILYFIGGIPSITYDDMDQIQINIKYSQQITDAKLKNTLLSF
                     VNSELISQDEKETFLNELELTKTSFTTQFNTELENIRKKAEELSKKAEVDPYHYESAS
                     KAANRLHQELLIASQLYFYHKTPEAYENFKQTCQREIERARPVLEQHRGWKPLLVNVG
                     AAIIGLGVLYLLAASINYYKTEGRHFFFHFETDSLQKLENLNVAQSQVLRI"
     gene            complement(1420..1821)
                     /locus_tag="ACF2SP_003101"
     CDS             complement(1420..1821)
                     /locus_tag="ACF2SP_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360849.1"
                     /translation="MQESIHSLFLAQAIGLYLLIVGIIMLSRASYYRELLTHLKEGSA
                     TIVTAGSLGLIIGICLVLVHNIWVPESEVLVTLVAWLILIKSILWLGFPEFMVKCSQK
                     AYTGTGYYVISIVVLIIGILLITHGFYLFSH"
     gene            complement(1907..>2387)
                     /locus_tag="ACF2SP_003102"
     CDS             complement(1907..>2387)
                     /locus_tag="ACF2SP_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFD2360850.1"
                     /translation="TGYPVEILDKIDRKILNILQKNNQITNQALADIVGISAPPCFRR
                     VKRLREEKIIVNDVALVDPFKVGRPLIVFVNITLEKQREDLLTHFERKMHEEPEVMQC
                     YFVSGDTDYLLIIHVKDMNHYNEFARRVFANEPNIRKFRSSFCLNRTKYNTQVVLDE"
BASE COUNT          753 a          419 c          425 g          790 t
ORIGIN      
        1 tttgcgtgat agagcatgtt ttgatgcagc gaatgcaatg agccaattgg tagccaagct
       61 cactacctac accaattatc ttgaaaaaaa tccagatcca tttttaataa aacgttataa
      121 agactgctgc atcgtagaaa tggatttggc taatttgaca ttgcaaaatc accgagatgc
      181 cttatggtta gtggctgagt tatcaactgc tatccttttg cttggggtag ggtatttatt
      241 tgcttttggt ataaattatt actacacagg tcgtatggga ttattttctc aaaccaggtc
      301 tgagaaattg gtggaggaaa tgaaaagctc ggtattgggt attgcggttg gaaattaaaa
      361 atctgtattt cccggattat aacagaatcg tctaatccgg gttgatcatg tttagattct
      421 taacacttga gattgagcca cattcaggtt ttctaatttt tgcaaggagt ccgtttcaaa
      481 atgaaagaaa aagtgacggc cttctgtttt ataatagtta atggaagctg caagcaaata
      541 gagaactcca agacctatga tagccgctcc tacattaact aacaaaggct tccatcctct
      601 atgctgttcc agtacaggtc gagctctctc aatttctctt tgacatgttt gcttgaaatt
      661 ttcgtatgcc tcgggtgttt tatgataaaa atacagttgg gaggctatta ataattcctg
      721 atgcagtctg ttagctgctt ttgacgcaga ctcataatga taaggatcaa cttctgcttt
      781 tttgcttaat tcctcagcct tttttctgat attttccaat tcagtattaa attgggtagt
      841 gaatgaagtt ttggtgagct ccaactcatt taaaaatgtt tctttttcat cttgagagat
      901 taattcagaa ttaacaaaag aaagtaatgt atttttgagt ttggcatcag ttatttgttg
      961 agaatactta atattaattt gaatctgatc catgtcatca taggtgatgc tagggatacc
     1021 tcctataaaa taaagaatgt acagattatt gaaaagagca gtaattcttt gcagcgcctc
     1081 tctattttta ggacaaatgg ttagaacatt atgagctaga ttgatatcaa ttttttcaca
     1141 agaatgttta aacgatgaca tactaaacct catataattc agagatttcc ctatgcaaca
     1201 attcatttcc attgaaggca ggtcaagtat atgtagacaa gcttaagatt ttattaagaa
     1261 gcgccttaaa tttaacaatt tttcaaaaaa actttacgat tttttgaaat gcaaaaaata
     1321 tcattaacac ttgcagattt cttttataaa aacctacaca cttcatccac ttgatggtta
     1381 ccttgctgaa agcagagttg cctatgaaag caggtaaaat caatgggaaa acaggtaaaa
     1441 tccgtgggtt attagcagaa tcccaataat taataccact atggaaatca cataataacc
     1501 tgtccctgta taggcttttt gactacattt caccataaac tcgggaaatc caagccataa
     1561 tattgattta atcaaaataa gccacgctac cagtgtaact aatacctctg attcaggaac
     1621 ccaaatgtta tggaccagta caaggcaaat accaataatt aaccccaagc ttccagccgt
     1681 tacaatagtg gcactgcctt ctttgagatg agtcaacaat tcacgataat atgaagctcg
     1741 agaaagcatt ataattccca caataagaag atagaggccg attgcttgtg ccaaaaataa
     1801 agagtgaatt gattcttgca tggttggctc cttgtgattc cgctcctaaa ttaattatag
     1861 tacatgcggg attggatttg ttaaccaggc aggtacttgc tagttactac tcatctaaaa
     1921 caacttgtgt attatatttg gttctattaa gacaaaagct gcttctgaat tttctaatat
     1981 tgggctcatt agcgaatact cgtctggcaa attcattgta atgattcata tctttgacat
     2041 gaataataag caggtaatct gtgtctcccg agacaaaata gcattgcata acctcaggct
     2101 cttcgtgcat ttttctttca aaatgagtta gcagatcctc tctttgtttt tcaagagtaa
     2161 tattgacgaa cacaattaat ggtctgccta ctttaaatgg atcaaccaaa gcaacatcgt
     2221 taacgataat tttttcctct ctcaggcgtt tgactcttcg aaagcagggt ggggcagaga
     2281 ttccaactat atctgccaat gcctggttag taatttgatt atttttctga agaatattta
     2341 gaatttttct atcaatttta tcaagtattt caactggata tcctgta
//
LOCUS       DAXLDT010000284         2334 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXLDT010000284 DAXLDT010000000
VERSION     DAXLDT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.285"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            2..424
                     /locus_tag="ACF2SP_003103"
     CDS             2..424
                     /locus_tag="ACF2SP_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD2360851.1"
                     /translation="MVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADI
                     EQVQTSYGPMLELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVINRPPRD
                     GHMAFIRSPDNISIELLQKGGPLPKQEPWLSAPNIGHW"
     gene            516..1580
                     /locus_tag="ACF2SP_003104"
     CDS             516..1580
                     /locus_tag="ACF2SP_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360852.1"
                     /translation="MQILKSLSEGMGTAGGVAWTTFGLLFGSFGLTVGNSALTISLGV
                     ISASLFLLVAIPVTYWSYCNSYKENDELRHAIEEQENQIVHYLTEYLLATRQECLLKN
                     PEKYRNHYDKSVLAEDLKNRLLKTRSKRPVSFTTAQFLDCLLDDNHKNSILGMLTDAC
                     TMDKLVLKKKLLAIYRDSVFLQLDELSLFAKLKTGFIAFCAGFGSLAGCSSGSLGLFA
                     ALGIFPGFAAVPLVGLAIMGIACVFGLFLSGMSIDTAIQRSKEKQLFVQSEAIHHDLK
                     NLATLKTASVKAELKAQTKYQIRAKEGLTEQLARPKLLKRSSSFDSYFYRNHPLWNIS
                     HNPPEASKCSFENLGSSFSL"
     gene            complement(1601..>2334)
                     /locus_tag="ACF2SP_003105"
     CDS             complement(1601..>2334)
                     /locus_tag="ACF2SP_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HFD2360853.1"
                     /translation="SNQLTELFYIADRTVRESGFDITAKFGPFSAGILDYAPVDLNVL
                     IYQMENDLGIIYKILGNKENATIWTQKAEARAALINHYLWDDKAGYYFDYNFKTKQLK
                     PYIYATTFYPLWAGIASKDQAQSLVNNLPVLLTRGGLLTSCYIQGVQWDAPFGWAPLQ
                     YFAVLGLNRYGYKELALDIANRFVNTIHIGFQEAHTLFEKYDVQNMSIHTENKIQYSY
                     NTNVVGFGWTNGVYLVFNRLINANN"
BASE COUNT          701 a          408 c          466 g          759 t
ORIGIN      
        1 catggttaga gtttcgaatt tagaacaatc gctcgatttt tattgtaata aattgggttt
       61 gatagaagta aagcgaactg aaaatgccaa aggccgtttt accttggtat ttttagctgc
      121 tcctgctgat atagagcaag ttcaaacttc ttatggacca atgctagagt tgacttataa
      181 ctgggatcct gaggattaca ctgagggaag aaattttggc catttggcat atcatgtgga
      241 tgatatctat aaaatctgcc aacatttaca ggaatgtggt gtcgtaatta ataggccacc
      301 cagagatggc cacatggctt ttattcgttc tcctgataat atttctatcg aattattgca
      361 aaagggtggg cctttgccaa agcaggaacc ttggttatct gcacccaata ttggacattg
      421 gtaacctgac aatttcattc tggtagtgaa tagtggaaat aatttaatca tactaaaata
      481 gtatttcggg ccaaatggca atgttgaatc aatcgatgca aattttaaaa tcactatcag
      541 agggtatggg aacagcaggg ggggtggcat ggactacctt tggactgcta tttggttctt
      601 ttggactaac tgttggcaat tcggcactga ctatttcatt gggcgtcatt tctgccagtt
      661 tattcctatt agtcgcaata cctgttactt attggtctta ttgtaattcg tataaagaaa
      721 atgatgaatt acgacatgct attgaagaac aggaaaatca aatcgttcat tatttgacag
      781 aatatttatt ggcaaccagg caagaatgtt tgcttaaaaa tcctgaaaaa tatcgtaatc
      841 attatgataa gtctgtgctg gccgaagatc ttaaaaatcg attattaaaa accagatcaa
      901 aacggcctgt ttcgtttacg acagcacaat ttctagactg tctattagat gacaatcata
      961 aaaattctat tttgggtatg ttaacagatg cctgcacgat ggataagttg gtattgaaaa
     1021 agaaactatt ggctatttat cgtgatagtg tttttcttca gcttgatgaa ctttccctat
     1081 ttgccaaatt aaaaactgga tttattgcgt tttgtgccgg atttgggtcg ctagctggtt
     1141 gttccagtgg ttcattgggt ttgtttgcag ccctaggtat tttccccggt tttgctgcag
     1201 ttcctttggt tgggttggca attatgggta tcgcttgtgt ttttggtttg tttctgtctg
     1261 gaatgagtat tgataccgca atccaaagga gtaaagaaaa acagttattt gtccaaagtg
     1321 aagcaatcca tcatgattta aaaaatttgg ccacgttgaa aactgcatct gttaaggccg
     1381 agttaaaagc acaaaccaaa tatcaaatta gagctaaaga gggattgaca gagcaattag
     1441 caaggccgaa gttgctgaag cgatcttctt catttgatag ttatttttat agaaaccatc
     1501 ccttatggaa catttctcat aaccctccag aggcttcaaa atgcagcttt gaaaacctgg
     1561 gttcttcttt ttcactttag taaaaacaat tgattataag ttaattattc gcattgatta
     1621 acctattgaa aacgagatac acgccattag tccatccaaa acctacgaca tttgtattat
     1681 agctgtattg aattttgttt tcggtatgaa tactcatgtt ttgaacatcg tatttttcaa
     1741 aaagagtatg ggcttcctgg aatcctatat gaattgtgtt gacaaagcgg ttagcaatat
     1801 ccaatgctaa ctctttatac ccatatctat tcaaaccgag tacggcaaaa tattgcaatg
     1861 gagcccatcc aaaaggcgcg tcccactgca ctccctgaat ataacaactg gttaataacc
     1921 ctcctctggt taatagaacg ggcaagttat tcacaagcga ttgagcttgg tctttggatg
     1981 caatacctgc ccacagggga tagaaagtag tggcataaat atagggtttt agttgttttg
     2041 ttttaaaatt ataatcaaaa taatatccag ccttatcatc ccaaagataa tggtttatta
     2101 gtgcagccct agcctcggct ttttgagtcc aaatagtggc attttcttta ttgccaagga
     2161 ttttataaat tatacccaga tcattttcca tttgataaat taacacattc agatctactg
     2221 gagcatagtc cagaatgcct gcgctgaaag gaccaaattt ggcagtaatg tcgaaaccgg
     2281 attctctgac tgttcggtca gcaatataaa acagctccgt taactggttt gatg
//
LOCUS       DAXLDT010000285         2321 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXLDT010000285 DAXLDT010000000
VERSION     DAXLDT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.286"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..262)
                     /locus_tag="ACF2SP_003106"
     CDS             complement(<1..262)
                     /locus_tag="ACF2SP_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360854.1"
                     /translation="MKKINVLLYLLTMLTISFATISHGASEDTDTTNPTKYIYRGSSI
                     TLEELATVADLSTARDQKGNPVTLEEESYQIGSFTRGTVTIIP"
     gene            582..>2321
                     /locus_tag="ACF2SP_003107"
     CDS             582..>2321
                     /locus_tag="ACF2SP_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD2360855.1"
                     /translation="MKGLEKQCCSKERILTQSQELQEGGDFSCCVPVESTSSNSFFSF
                     FTRLTQANLAKWTAGISPAAIGSSYSTWLWQLAQSPGVLWELAFYPVLHAKDCINNIV
                     CAERAADGKDVRFKKNSWQPMPWRLFAEGFLQMEDWWRRATTDVPGLPKPVERTVSFW
                     ARQCLDALSPSNFAWSNPDLFHEAMRTGGLNLIQGTQIALEDWLEKLAGAPPTGSEHF
                     IPGQHVAITPGRVVFQNHLIELIQYESHTKTVYKEPILIFPAWIMKYYILDLSPHNSM
                     VKWLVSQGHTVFIISWRNPDKEDRDLGMDDYYRQGAMAAIDTVSTLLPGTKINLMGYC
                     LGGTLAMITASAMGRDKDERLNSLTLLAAQGDFTEAGELMLFVTESQVDFLKSMMREQ
                     GYLDTKQMAGSFQMLRAYDLIWSKMVQDYMHGMRRGMIDLTAWNADATRMPYKMHSEY
                     LEKLFLRNDFAEGRYTVEGKPVAAENIERPVFAVSTEKDHVAPWQSVYKIHLMVKGDV
                     TFVLTDGGHNAGIISEPGHLGRSYHVHEHTQGETYLNPESWLAMANVREGSWWQEWHE
                     WLVRQNSKKLIPPP"
BASE COUNT          643 a          454 c          550 g          674 t
ORIGIN      
        1 tgggaatgat agtcactgta cctcttgtaa aagagcctat ttggtagctt tcttcttcta
       61 aggtgactgg gttgcctttt tgatcccgtg ctgtggataa atcagcaaca gttgctaatt
      121 cttcaagagt aatagaggaa ccacgataaa tatatttcgt tgggtttgta gtgtctgtat
      181 cttcgctggc tccatggctt atagtagcaa aggatatagt aagcatagtt aataaataca
      241 atagaacatt tatctttttc attagactgt ccttaatcaa atgatgtaat acaataatag
      301 atcacctgtt gctcaactac aaaatgctta tctcagagaa aacaataatt taattattaa
      361 atcgacgatt gctttgtcta aagagttagg attgaatttt gcggcagggg gtgtagaaac
      421 agaagaataa ctcaattttt taagcaaatc tccaagaggt cactctttcg cttttctatt
      481 ctttaagaag agtctcctta catgacattg atgcgctaaa tcttatgcta tgctgtatta
      541 tatcttgttg attattgggc gtaatgccca ggatgtcagc catgaagggg ctagaaaaac
      601 aatgctgttc taaggaaagg atactcactc agagccaaga attgcaagaa gggggggatt
      661 tctcttgctg tgtacccgtt gagagtactt cctccaatag cttcttttcg ttttttactc
      721 gattaactca agctaacttg gctaaatgga cagcaggaat aagtcctgct gccatcggct
      781 cttcctattc cacctggctt tggcaattag ctcaatcccc cggggtctta tgggaactcg
      841 ctttttatcc ggttttgcac gccaaggatt gcattaataa tatcgtttgc gctgagcgtg
      901 ctgcggatgg taaagacgtt cgttttaaaa agaacagttg gcagccaatg ccttggcgtt
      961 tattcgccga aggatttttg cagatggaag attggtggcg acgtgccaca acggacgtac
     1021 caggattacc caaaccggta gagcgcactg tttcgttctg ggcacgccaa tgccttgatg
     1081 ccctgtcacc ttccaatttt gcttggtcta atcctgattt attccatgaa gctatgcgca
     1141 ctgggggact taatcttatc caaggcaccc aaatcgcgtt ggaggattgg ttggaaaagc
     1201 tggcgggtgc accgcctacg ggctctgaac attttatccc aggtcaacac gtggccatta
     1261 caccaggacg agtggtgttt caaaatcatt tgattgagct cattcaatac gaatctcata
     1321 ctaagacggt gtataaagag cctatcttaa tctttcccgc ctggattatg aaatattata
     1381 ttttggattt gtcaccgcat aactccatgg tgaagtggct cgtaagtcag ggacacaccg
     1441 tattcatcat ttcttggcgc aatcctgaca aagaggaccg tgatttaggg atggatgatt
     1501 attataggca aggagctatg gccgccattg atacggtatc gactcttttg ccaggaacca
     1561 aaatcaatct gatgggttat tgtttaggtg gcaccttggc tatgattact gcatcggcga
     1621 tgggaagaga caaggatgaa cgtttaaaca gcttgacact cttggcagcc caaggggatt
     1681 ttactgaggc cggggaatta atgctttttg tgactgaaag tcaagttgac tttcttaaaa
     1741 gtatgatgag ggaacaaggg tatctcgata ccaagcaaat ggcaggttct tttcagatgt
     1801 tgcgagccta cgatttaatt tggtcaaaaa tggttcagga ttacatgcat ggaatgcggc
     1861 gagggatgat tgatttgacg gcttggaatg cggatgccac gcggatgcct tacaaaatgc
     1921 acagtgaata tcttgaaaaa ctatttttaa ggaatgattt tgccgaagga cgttatactg
     1981 tagaaggaaa gccagtggct gctgagaata tcgagcggcc tgtttttgca gtgagcactg
     2041 aaaaagacca tgtagcgcca tggcaatccg tatataaaat acatcttatg gtgaaaggag
     2101 atgtaacctt tgttctcact gacggtgggc ataatgcagg catcatcagt gaaccgggac
     2161 atctcgggcg ctcttatcat gttcatgagc atacacaagg cgagacgtac ttaaatccgg
     2221 aaagctggct tgcgatggca aatgtacgag aaggctcctg gtggcaagaa tggcatgagt
     2281 ggttagtacg acaaaactct aaaaaactca ttccgccacc t
//
LOCUS       DAXLDT010000286         2320 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXLDT010000286 DAXLDT010000000
VERSION     DAXLDT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.287"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..648
                     /locus_tag="ACF2SP_003108"
     CDS             <1..648
                     /locus_tag="ACF2SP_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFD2360856.1"
                     /translation="KKNDEQFSPDRIEIIYFNDGIDYDQLDEKIEIYICAHGSDDDNV
                     TKLFNHSNPLVAESLDIKEVAERFERDILPISYWISTIHLYCCGTNNKNQMMAELLGH
                     SLLRPEKPIYHYSGSVSIVDEYGKQWSFANHVKIPVHLVAKRTFILNFFDEEQPHRAF
                     VKKAFQSKTYKELLAKKEDSFFAKVKENRASVLLSKRLGKKTQDGEENSLLRKGN"
     gene            913..1554
                     /locus_tag="ACF2SP_003109"
     CDS             913..1554
                     /locus_tag="ACF2SP_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360857.1"
                     /translation="MKEQFLQPKDLNTLLRSVEKPIQDLLDLNLKTFKSVSYVTPIEL
                     FNVLKPEEVLEKNMNVFIQNSQKAMSYMLNVFQIMEHHWINIYDQMAENPNEKKASPA
                     IDKANRKVDTSTPKSSASKKAVTAKGKTKPAAVQSKSKTSKAKPKISSPTKVKTNTQE
                     RPAAVKQQSQMPLSSLQKDMEKQGINTPIELKNSVAILKNETKDIVPSNNKLK"
     gene            complement(1947..>2320)
                     /locus_tag="ACF2SP_003110"
     CDS             complement(1947..>2320)
                     /locus_tag="ACF2SP_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFD2360858.1"
                     /translation="IRAQQKVDHGKEVLMFANWRLYPASRQVFGENNEELQLSAGEYD
                     LLLTFVQQPQRVLDREFLLQITKNTDLVPFDRRIDVQISRLRQKIEIDKKKPALIKTI
                     RNGGYMFTARVVTLKESDATL"
BASE COUNT          823 a          376 c          427 g          694 t
ORIGIN      
        1 aaaaaaaacg atgagcaatt ttcccctgat cgcattgaaa taatttattt caatgatggc
       61 attgattatg atcaattgga tgaaaaaatt gagatatata tttgtgctca tggttcagat
      121 gatgacaatg taacaaaact ttttaatcat tccaatccat tagttgcaga aagcctcgat
      181 ataaaagaag tggcagaacg ctttgaaaga gatattttac ctatatcgta ctggatatca
      241 acgattcatc tttattgctg cggaactaat aataaaaatc agatgatggc tgaattatta
      301 gggcattcat tgttgagacc agaaaagcct atttatcact acagtgggtc agtttctatt
      361 gttgacgaat atgggaagca atggagtttt gcaaaccatg ttaaaatacc tgtgcatttg
      421 gtagctaaaa gaacgtttat tctaaatttt tttgatgaag agcaacccca tagagccttt
      481 gttaaaaaag catttcaaag caagacgtat aaagagttat tagccaagaa ggaagattct
      541 ttttttgcga aagtgaaaga aaatagagct tcagtgcttt tatccaaacg attaggcaaa
      601 aagacccagg atggtgaaga aaactctttg ttgagaaagg gtaattaaaa tttttacctt
      661 tgatacattt tagtttgcga taattcgctt tatcaaaagg cattctggaa agtgcgataa
      721 ccattgaact cagaataagt gtaagtattt tgatatgatg atgtcgttgc caagagttac
      781 aataagagta aaaacaagct aattagcgat gcgaatgatt tatggagtta caagaaaatg
      841 gcatttaata tttgacaaat tattttttta agctaatctt gtttttgtgc attgcaacat
      901 atggagagtg taatgaaaga gcaatttttg caacctaaag atttaaatac gttgcttcga
      961 tctgttgaaa aaccaattca ggatttattg gatctcaatc ttaaaacatt caaaagcgtg
     1021 tcttatgtta ctcctattga attattcaat gttttaaaac cagaagaagt cctggaaaaa
     1081 aatatgaatg tattcattca aaatagtcaa aaagcaatga gttacatgtt gaacgtattt
     1141 caaataatgg aacatcattg gattaatatc tatgatcaga tggccgaaaa tccaaatgaa
     1201 aagaaggcta gtccagcaat agacaaagca aatcgcaaag tagataccag tactccgaaa
     1261 tcatcagcca gtaaaaaagc cgttacagca aaaggtaaaa caaagcctgc tgcagtacag
     1321 tcaaaatcaa aaacaagtaa agcgaaaccg aaaatcagca gtccaacaaa agttaaaaca
     1381 aatactcaag agcggcctgc agctgtcaaa caacaatcac aaatgccctt atcttctttg
     1441 cagaaagata tggaaaaaca gggtattaac acgcctattg aattgaaaaa ctcagttgcg
     1501 atattaaaaa atgaaacaaa ggatatagtg ccttctaata acaaacttaa ataatgcttt
     1561 atcctgtttt cgttttgagc gcttttttga gaagtagcaa acgatacttg ataaatccgg
     1621 tatacccaaa agctagacat ggtagggtgc aatgccagca agtaaaggat aaggcgggca
     1681 ttgtgccaat aaagcaaata tcagttcata gtttttaagt aaacgttcat ggggcatgta
     1741 aattccgtca ttgcgaacga agtgaagcaa tccagtacag aattctaaca aaatatcgtt
     1801 ctggattgct tcacttcgtt cgcaatgaca gcatttattt catttgtttt tcaagagatg
     1861 gcataacccg tgaacgttta cgtttttaaa atgcgctcga taaataagat gaacgaattt
     1921 gtctaaataa accagataat aatcaattat aatgtggcgt cactttcttt aagggttaca
     1981 actcgagctg tgaacatgta gccgccatta cgaatggttt tgattaaagc tggctttttt
     2041 ttatctattt cgattttttg acgcaaacga ctaatttgga catcaatcct tcggtcaaaa
     2101 ggaaccaggt ctgtattttt ggtaatttgc aataaaaatt ctctatccag gactcgctgc
     2161 ggttgttgga caaaagtgag aagcaaatca tactcaccgg cgcttaattg caattcttca
     2221 ttattctccc cgaacacttg tctggaagct ggataaagtc gccagttagc aaacattaat
     2281 acttctttac catggtctac tttttgttgc gccctgatta
//
LOCUS       DAXLDT010000287         2294 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXLDT010000287 DAXLDT010000000
VERSION     DAXLDT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.288"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1778
                     /locus_tag="ACF2SP_003111"
     CDS             <1..1778
                     /locus_tag="ACF2SP_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFD2360859.1"
                     /translation="AVSRDAAMRAESIEKKSIAYHNVIDCDYKISPEISHIDSDIILN
                     WAEYAAILSFNFNFASIEDQLNQLHACYTEKGWGQMQNALHKSNNIEIIKAEKLKVKG
                     YKDGEAQIIEALDNRWKIILPLKVVYENDKKRVTHFLNAYLTIGWKNKDSLGIMQMIA
                     IPRLAPLAQKVTPADEVIKSMGIILAEKKKASLDSARKLTDSFFVPLFSNHGERSKRN
                     LTLSNRAAQRVAIIQPMPNLNPTHNLVRMRSFDMPESTKTPYLALSQQRSLINDSDSN
                     KSTPSDFGNKGRKLYPNWLSQIGLHSSKLQLNSLITRINPLPLWRVFSQYQQSIRDKS
                     DNIEAISKQKPILSAQIEGEPQFIEVKENQWDIKLPIKVVYQKDNERITQRLDVNLAL
                     DQKMINNLTVKTKSVDSNKVLQSLKSILASSSLQLSPQLFLDSQNIQPKQAEQSQSKG
                     VVSCQYKIPEEIIKTDKNIILQWAEYAAMKSFHFDYTALEAELKELESCYTEKGWSEF
                     NAAMDKSGNLNAIKTQRLTMNSQIDGQARLIESQDNQWKIQLPLKVIYKNDRTQVSQF
                     LDIQLIVGRKMNDEFGIVQLIAVLK"
     gene            complement(1891..>2294)
                     /locus_tag="ACF2SP_003112"
     CDS             complement(1891..>2294)
                     /locus_tag="ACF2SP_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360860.1"
                     /translation="LNSLKQQILDITSQRGHTKKELTFWGKIQIIFKAIANGVENVLG
                     ERHEASKLEFLGNLFHYPYETIKAELSGQAEHANVLHKVISRCEQQITTSCLIEKQSS
                     MKGRLREIISPKPEESLENSNSQLSHLTVSI"
BASE COUNT          778 a          375 c          424 g          717 t
ORIGIN      
        1 acgctgttag cagagatgcc gcaatgcgag ctgaatccat tgaaaaaaag agtattgctt
       61 atcacaatgt tattgattgt gattataaaa tctcacctga aatttctcat attgattcgg
      121 atatcatttt aaattgggct gaatatgcgg ctattctgtc ttttaatttc aattttgcgt
      181 ctatagagga ccaattaaat caattgcacg cttgctatac tgaaaagggg tgggggcaaa
      241 tgcaaaatgc tttacataaa tcaaataata tagaaatcat taaagcagaa aaattaaaag
      301 taaagggcta taaagatggg gaggctcaaa taatagaggc tttggataat cgatggaaaa
      361 ttatattgcc tttaaaagtt gtttatgaga atgataaaaa aagagtgaca cattttctta
      421 atgcctatct gaccataggc tggaaaaata aagatagttt ggggattatg caaatgattg
      481 ctattcctcg tctggcgcca ctagctcaaa aagtgacgcc agctgatgag gtaattaaaa
      541 gtatgggcat aatactggct gagaaaaaaa aggccagttt agatagtgca agaaaattaa
      601 cagattcttt ttttgtgcct ttattttcaa atcatggaga acgttctaaa agaaatctta
      661 ctttgagtaa cagggctgcg caaagagtgg caattattca accaatgcca aacttaaatc
      721 ccactcataa tcttgtgaga atgcgatcat ttgatatgcc tgaatctaca aaaactccat
      781 atcttgcttt atcccaacag agatccctta ttaacgattc tgattctaac aaaagcactc
      841 cgagtgattt tggcaacaaa ggtcgaaaac tctatccaaa ctggttaagc cagatcggtt
      901 tgcattcttc taaattacaa cttaattcat tgattactcg aattaatccc ttgcccttat
      961 ggcgtgtttt ttctcaatat caacaatcca taagagacaa atcagataat attgaggcaa
     1021 tcagcaagca aaagccgatt ttaagtgctc agatagaagg agagccccag tttatcgagg
     1081 ttaaagaaaa tcaatgggat attaaattac ctataaaagt tgtttatcaa aaagacaatg
     1141 agagaatcac tcaacgcctt gatgttaatt tagctttaga tcaaaagatg ataaataatt
     1201 tgacggtaaa gactaagagc gttgattcta ataaggtgct acaatcttta aaatcgatac
     1261 tggcctcttc ttctcttcag ctatcacctc aattgtttct cgactctcaa aacattcaac
     1321 ccaaacaagc tgagcaatca cagtcaaaag gtgttgtttc ttgtcagtat aaaatccctg
     1381 aagaaattat taaaacagat aaaaatatta ttttacaatg ggccgagtat gctgctatga
     1441 agtcatttca tttcgattat acggctctgg aggcagagct aaaggagttg gaatcatgtt
     1501 atacggaaaa aggttggtct gagtttaatg ctgctatgga taaatcaggt aacctaaatg
     1561 caattaaaac ccagagacta accatgaata gccaaattga tggtcaagca cgattaattg
     1621 aatctcaaga taatcagtgg aaaattcaat tacctttgaa agtgatatat aaaaatgata
     1681 gaacacaagt ttctcagttt cttgatattc aattgattgt aggcagaaaa atgaatgatg
     1741 aattcggtat cgtgcaactg attgccgtac tcaagtgaag aataactcat attggctgtc
     1801 ttaatgaagt gtcgtgatca atatgaacac caataaataa tattagctct acctacatca
     1861 acgagcataa atgcggtaga gcgtaaattt ttagattgac acagttaggt gtgagagctg
     1921 tgaattacta ttttctagag attcttcagg tttgggtgat attatttctc tcaacctacc
     1981 cttcatactg gattgctttt caattaagca agaagttgtt atttgttgtt cgcatcttga
     2041 aattactttg tgtaatacat tggcatgttc agcttgacct gaaagctcag ctttaatagt
     2101 ttcgtagggg taatggaata aattaccgag aaactctaat ttgcttgctt catgacgttc
     2161 tccaagaaca ttttcgacgc cattggctat agctttaaag ataatttgta ttttacccca
     2221 gaaagttaat tccttcttgg tgtgtcctct ttgtgatgta atatctaata tttgttgttt
     2281 cagtgaattg agta
//
LOCUS       DAXLDT010000288         2283 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXLDT010000288 DAXLDT010000000
VERSION     DAXLDT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.289"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            110..>2283
                     /locus_tag="ACF2SP_003113"
     CDS             110..>2283
                     /locus_tag="ACF2SP_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFD2360861.1"
                     /translation="MDNSFVFNETTLKRVPHFLVLIIILLLGLPYIGLNWGLDFSNLA
                     ITINDGKIAGSQLIESQIRGYFRQTLLQWSGFSLAAVTVLLAFTQYRLSNDKIALIIG
                     LAILFSGSIEALHTLVIDGLALQGPEKNNLDALIWVFSNSVSGLILMTGLSLLVHAGA
                     NKPVRLVTFILLTTFLVLAAIAVIYYAAVIIQIPDMWLYDSYVSRPFELISVFIYLML
                     MIIVYPKIYASYPTILSDSVFYMSLTQVVLAIYIMLLSTTPYDSAFNIAYFLKVVVYF
                     IPCTCFVINYVFSYSAVLEGQKNLKIKQEELKYMASHDPLTNLFNRREFEDLLDKSIA
                     NAQRNNSSLALLLIDLDNFKAINDTFGHFHGDELLKQFANRLVLLVRKGDLLSRVGGD
                     EFTLISLNIESPSSARHLAERILNELNSPYTINGKLITVTVSIGISIFPDDGNTTEDL
                     LRKADLAMYKAKNSGKNTYQFYTEQLGYSQHRESELESHLRKALQNDEFELYYQPKFN
                     LINMEIIGAEILLRWNNKALGNVSPNEFIPVAENTGLITELSQWVLEKSCAQILHWTN
                     QYNKMLSFSINISPVQLTNPQFPKILERILQEYQYPAEYLELEITENSLMGDQDEISA
                     MLNVISGLGIKLSLDDFGKEYSSLNRLKTLPIDSLKIDKTFISDIHNEHEKVVIIDII
                     IKLANELGMSIVAEGIETIPQLKYLVSRKCHFGQGFLLSKPLT"
BASE COUNT          724 a          409 c          385 g          765 t
ORIGIN      
        1 tggtaaatac gaggatgact agattaacag gatataaaat cagcaatttt gccaataaca
       61 ggcacatttt atgctaattt taaaatataa gaattttgag atacaaaaaa tggataattc
      121 atttgtattc aatgaaacca ctctaaaacg tgtaccccat tttttggtat tgattattat
      181 tttactgtta ggtctgcctt acattggtct aaactggggt ttagacttta gtaatttagc
      241 cattacaata aatgatggga aaatagcagg ctcccaactc attgaatctc aaattcgcgg
      301 ttacttccga caaactctct tacaatggtc tggattttct cttgccgcag tcaccgtatt
      361 attagcgttc acccagtacc gattgagtaa tgataaaatt gctttaatta tcggtcttgc
      421 cattctattc tctggctcta ttgaagcgct gcataccctt gttattgatg gcctggcttt
      481 gcaaggtccc gaaaaaaata acctggatgc tttaatctgg gttttttcaa acagcgttag
      541 cggtttaatt ctaatgaccg ggctttcttt actggtgcat gcaggcgcta ataaaccagt
      601 gagattggtg acttttattc tactgaccac ttttttggtc ttggctgcta ttgcagttat
      661 ttattatgcc gctgtaataa ttcaaattcc tgatatgtgg ctttatgatt cttatgtttc
      721 cagacctttt gaactcatat ccgtctttat ctatcttatg ttgatgatta ttgtttaccc
      781 aaaaatctat gcatcttacc cgactatttt atctgattct gtattctata tgtcactcac
      841 ccaagtcgtg ttagctatct acatcatgtt attatcaaca actccgtatg acagtgcctt
      901 taatatagca tattttctta aagtggttgt ttattttata ccttgcacct gttttgtaat
      961 taattacgtt ttttcatact cagcggtact ggaagggcaa aaaaacttaa aaataaaaca
     1021 ggaagagtta aaatatatgg cctctcatga tcctctgaca aacctgttta atcgcaggga
     1081 atttgaagac ttgcttgata aatccattgc taatgcccaa agaaataact cttccctggc
     1141 tttactgctc attgatttag ataatttcaa ggcaataaat gacacatttg gtcattttca
     1201 tggcgatgaa cttttaaaac aatttgccaa tcgcctggtt ttactagttc gaaaaggtga
     1261 tcttttatcc agagtgggtg gagatgaatt tacattaatc tctctgaata tagaatcccc
     1321 ctccagcgca cggcatctgg cagaacgaat tttaaatgaa ttaaacagtc cctatactat
     1381 caatggcaaa ttaatcacag taacagtcag tataggtata tctatttttc ctgatgatgg
     1441 aaatactaca gaagatctgt taagaaaagc ggacttggct atgtataaag caaaaaactc
     1501 agggaaaaat acctatcaat tttatactga acaactaggt tattcacagc atagggaatc
     1561 cgagctggaa tctcatcttc gaaaagcatt acaaaatgat gaatttgaac tttattacca
     1621 acccaaattt aacctgatta atatggagat tattggcgca gaaattcttt tacgctggaa
     1681 taataaggcc ctgggaaatg tatctcctaa tgaatttata cctgtagctg aaaacacggg
     1741 attaataaca gaattgagtc aatgggtatt agaaaaatcc tgtgcacaaa tattgcattg
     1801 gactaatcaa tacaacaaga tgctttcctt ttccattaac atttctccag tacaattgac
     1861 aaaccctcaa tttcctaaaa ttcttgaaag aatattacag gaatatcaat atcctgcaga
     1921 atatcttgaa cttgaaatta ctgaaaactc tttaatgggg gatcaggatg aaatctctgc
     1981 gatgcttaat gtaatttccg gattgggaat aaagctgtca ttggatgatt ttggcaagga
     2041 atactcctct cttaatagat taaaaactct ccctattgac agcttaaaaa ttgataaaac
     2101 atttatctct gatatacaca acgaacatga aaaagtagtc attatagaca ttattattaa
     2161 attggctaat gagttaggaa tgagtattgt tgccgagggt attgaaacta ttcctcaatt
     2221 aaaatatctt gtgtcaagaa aatgtcattt tggtcaaggt ttcctcctga gtaagccctt
     2281 aac
//
LOCUS       DAXLDT010000289         2270 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXLDT010000289 DAXLDT010000000
VERSION     DAXLDT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.290"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..2213)
                     /gene="lem7"
                     /locus_tag="ACF2SP_003114"
     CDS             complement(<1..2213)
                     /gene="lem7"
                     /locus_tag="ACF2SP_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem7"
                     /protein_id="HFD2360862.1"
                     /translation="MADSRSSIQSILEVDKRFDEKYYNYLVDDNYLIPAEAAVIANGL
                     AIFKEEFERGNLFKQRKDNHDSFTHEQILQYYLELALTCGRLEIIKYLIDELHVSLSE
                     EKLLHFLFLHSPYEGNKYFENIVEIYNYLEKKKEIGSNKQLIEEIFLKEAKKGHFNAV
                     KAIIELKIIPDMNVTDPDGHTAFFYCILESKKKRFSFNPQELGAVDVILSVRDKEILE
                     LIELVDATILAMEDKNEEKLVKLRKVFTDSIDKIPEALIEDCLSRAIELNDVKMIQLL
                     LPVCNPDLKFLMLGKVNSVEAGMLLIEHFISTGFVIPEARSLEGYAHRLGLNEYTAGA
                     EQANRFLLHAVAGFVNRTDNSKDFKTILRALQKTVLLNSIANVEVARKILYQTYLEGE
                     PLLLTVGWTQHGVGLSILWDKESNKTYLVFSNRGQGGIASCLNAKGLIRKEEPKAGSV
                     IYEIDGKLDEGFFSHFEIGENKLGLFRYGKKAFEKDIRTCLKDARVLPHFIFAAAQNY
                     ATCGYVNTKQSIRGLLFVFAMTRGDSLSEELIDRMSRQYKEFTAHDRNVACSELIEDF
                     QRKIKFDSKHIQKEYQDFIVKLILSHCTSDKPEDIERTQRLFVVLPEEIQEKLAHLIP
                     CHIVARKEDKSSTLQNLSLFKHQEEPSSQNDYFLHRPIGVIPDSVDNLLNKYHEMNTL
                     LKFLESHGYTEKDIPKIVLENNSVRIHTKSDRDFKYKVKDLFPKDIQIVNVQGRY"
BASE COUNT          696 a          481 c          335 g          758 t
ORIGIN      
        1 tcatatcttc cctgaacatt aacaatctgg atatctttgg gaaaaagatc ttttacttta
       61 tacttaaaat ctctatccga cttagtgtga attcggactg aattattttc caaaacaatt
      121 ttcggaatat ctttttctgt ataaccatgg gattctaaaa atttaagtag tgtattcatc
      181 tcatgatatt tatttagtaa attatccaca gaatcaggga ttactccgat cggcctatgc
      241 aaaaaatagt cattttggga ggagggttct tcctgatgtt tgaagagact cagattttga
      301 agggttgatg acttatcttc tttccgtgct actatatggc aaggtatcag atgagccagt
      361 ttttcctgta tctcttctgg taacactaca aataatcgtt gggttcgctc aatatcttct
      421 ggtttatccg atgtacaatg cgataaaatc aatttgacta tgaaatcttg atactccttc
      481 tgaatatgct tggaatcaaa cttaattttc ctctgaaaat cttcaattaa ctcggaacaa
      541 gcaacatttc tatcatgagc tgtaaattct ttgtattgac gactcattct atcgattaac
      601 tcttcactta aagaatcgcc tcttgtcata gcaaaaacaa ataataaacc gcgtatggat
      661 tgttttgtgt tgacgtagcc acaggtggca taattctgtg ctgcagcaaa aataaagtgg
      721 ggtaatactc gggcatcttt aagacatgtc ctgatgtctt tttcaaatgc tttcttccca
      781 tatctaaaaa gacccaattt attttcccct atctcaaaat gagagaaaaa gccctcgtcc
      841 aacttaccat ctatctcata gataactgaa cctgctttcg gctcctcctt cctaattaaa
      901 cccttagcat taagacatga agcaattcct ccttgccctc ggttagaaaa tacaagataa
      961 gttttattgg actctttatc ccataatatg gataaaccaa caccatgttg cgtccatccc
     1021 accgtaagca acagaggttc gccttccaaa tacgtttgat ataaaatttt ccgcgccacc
     1081 tccacattcg ctatgctgtt taatagcact gtcttttgta gcgcccttaa aatagttttg
     1141 aagtctttac tattgtccgt ccgattgaca aatccagcga ctgcatgtaa caaaaatcta
     1201 ttcgcctgtt cggcaccagc agtgtattcg tttaacccca acctgtgcgc ataaccctcc
     1261 aaactcctcg cttcgggtat aacaaatcca gtacttataa aatgctctat tagtagcata
     1321 ccggcttcaa cggaattgac cttaccgagc atcaagaatt ttagatcagg attacaaaca
     1381 ggtaacagaa gttgaatcat cttgacatca ttcaactcaa ttgctcgcga caaacagtct
     1441 tctatcaacg cctcaggaat tttatctata gagtcagtaa acactttcct taatttaacc
     1501 agtttttctt catttttatc ttccattgct aaaatggttg catcaaccag ctctatcaat
     1561 tcaagaattt ctttatcccg aactgataaa ataacatcca ctgctcccaa ttcttgagga
     1621 ttaaaggaaa atctcttttt cttgctttcc aaaatacagt aaaaaaatgc tgtatggcca
     1681 tcggggtcgg taacattcat atctggtata atcttaagtt ctataatcgc tttgaccgcg
     1741 ttaaagtgac cttttttagc ttcctttaaa aagatctcct ctatcaactg cttattactt
     1801 ccaatttcct tcttcttttc caaataatta taaatttcta cgatgttttc aaaatattta
     1861 tttccttcat acggactgtg caaaaataga aagtgcaaca gtttttcctc ggataaagaa
     1921 acatgaagct catcgataag gtacttaatg atctcaagac gcccacaagt aagagccaac
     1981 tccaaataat actgaaggat ttgttcatga gtgaaagagt catgattgtc ttttctctgt
     2041 ttaaataagt ttccacgctc aaactcttct ttgaatattg ctaatccatt ggctatgact
     2101 gcagcctcag ctggaattaa ataattatca tcaaccaaat aattataata tttttcatca
     2161 aatcgtttat cgacttccaa aatactttga atcgatgatc tggaatcagc caaaatttta
     2221 tctcctcttg atggttatct ttaagtataa tcaaaaaata accagtcaga
//
LOCUS       DAXLDT010000290         2176 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXLDT010000290 DAXLDT010000000
VERSION     DAXLDT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.291"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1049
                     /locus_tag="ACF2SP_003115"
     CDS             <1..1049
                     /locus_tag="ACF2SP_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HFD2360863.1"
                     /translation="TVLKNIQIGAEQFNLKNSPFSVQIKAKLTDAAFLQTAKANINFK
                     GRVSLSPSIIDELNSGISKSSIEGQLQIQNILLNQFAIKKINTTLKTHKRDIQFNPLT
                     LSLYNGESIGDMDYVIATQQLLINQTATNLDGKQLITSLLKHPAISGNLDYSIHASIP
                     LKALSIESLVSKGTITLKDGEVYNINLDQLLNNLKVKLNSLMTETPDNIKKLAQSADW
                     DQNKNTQGNTKFKLANFKFQLQTGAISSDSFLLQTDKLQVNGEGRINLLNQEIYSNIK
                     ATLNDNSTDNTLQKIQQALGGYFPLVVSGTVENPIVLPDFKAISPVLSSLAIKSALTK
                     PLKIIQKPLKELIH"
     gene            1046..2113
                     /gene="mutY"
                     /locus_tag="ACF2SP_003116"
     CDS             1046..2113
                     /gene="mutY"
                     /locus_tag="ACF2SP_003116"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFD2360864.1"
                     /translation="MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIML
                     QQTQVQTVIPYFNRFIEHFPDIFLLANADEDEVLSLWSGLGYYSRARNLHNTAKIISD
                     QYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPE
                     QAQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNK
                     KQHLYPTKKIKKQRPILSQQFLVLHNRQNQVYLEKRPPTGLWGGLWCLPSINNQTCPI
                     EHIQLFYRLQGDSPKLITRFKHSFSHFHLEITALSIRIESTNNFISESRGQWFTKETL
                     PTLGLAKPTTLILSKLMETGA"
BASE COUNT          770 a          424 c          360 g          622 t
ORIGIN      
        1 ctactgtctt aaaaaatatt caaattggag cagagcaatt taatttaaaa aactcgccct
       61 tttcagtaca gattaaagcc aagctgactg atgctgcttt tttacagaca gccaaagcta
      121 atattaattt taaaggtcga gttagtctat caccctcaat tattgatgaa ctcaattcag
      181 gaattagtaa gtcttccata gaaggccaac tgcaaataca aaatatttta ttgaatcaat
      241 ttgctatcaa aaaaatcaat accactctta aaactcataa aagagatatc caatttaatc
      301 cacttacttt gtcactctat aatggggaat cgataggaga tatggactat gtcatcgcta
      361 cacaacaatt gttaattaat caaactgcaa ccaatttaga tggaaaacaa ttaatcactt
      421 ctttattaaa acatcctgca attagcggta atttagatta ctcaattcat gcctcaatac
      481 cattgaaagc actcagtatt gagagtcttg ttagtaaagg aaccattacg cttaaagatg
      541 gagaagtcta taatattaat cttgatcaat tacttaataa tctaaaagta aaacttaatt
      601 cgcttatgac tgaaactcca gataacataa aaaaactggc tcaatcagcg gattgggatc
      661 aaaataaaaa cacgcaaggc aataccaaat ttaaactagc aaattttaaa ttccagttac
      721 aaaccggagc aattagcagt gattcatttt tgctgcagac tgataaactc caggtgaatg
      781 gagaagggag aattaatctg ctaaatcaag aaatatactc caatattaag gccacgttaa
      841 acgacaatag tacagacaat actttgcaaa aaattcaaca agcattgggc ggctatttcc
      901 cactggtagt atctggcaca gttgaaaatc ccatagtttt gccagatttt aaagcaatta
      961 gccctgtatt aagttcgtta gcgatcaaat cggctctgac aaaaccttta aaaataatac
     1021 aaaaaccact taaagagcta atccattgag tacaaactct ttaaatcaac ttttcagtca
     1081 attactgctt gattggtatg acctgcatgg acgcaaagat ttgccatggc aattaccccg
     1141 ctctccctac agagtttggg tatctgaaat catgttacaa caaactcaag ttcaaactgt
     1201 tattccctac tttaatcgtt ttatagaaca tttccctgat atttttttgc ttgctaatgc
     1261 agatgaggat gaagtgctct ctctatggtc ggggttaggc tattacagtc gcgccagaaa
     1321 tcttcacaac acagcaaaaa taattagtga ccagtataat ggcgtatttc ctgaagatct
     1381 gaacatttta gttcaactgc caggaatagg accatcgaca gcagcagcga tactttcaca
     1441 agcttttaat aaaccagctg ctattttaga tgggaatgtg aagcgagttt tgtctcgatt
     1501 ttttttaatc gaaggctggc cagaacaagc ccaggttaaa aaaaaactat gggggcttgc
     1561 gtcttcttgt atgcccaatg aacggtgtgc agattataca caagcaatta tggacttagg
     1621 tgcaacatgc tgcacaaata aaaatcctca gtgtttgcgt tgtcctgtta aaaatcattg
     1681 cctggccttt cataataaaa aacaacatct ttatccaaca aaaaaaataa agaaacagcg
     1741 cccaatatta tcccaacagt tcttggtgtt gcataataga caaaatcagg tttatctgga
     1801 aaaaagacct cctacagggt tatggggagg cttatggtgt ttacccagta taaataatca
     1861 aacttgccca attgagcata tccaactctt ttacaggttg cagggagata gcccaaagct
     1921 aattacccgg tttaaacaca gctttagcca ttttcatttg gaaataacag cattgagcat
     1981 aagaatagaa tctacgaata attttatctc tgaatccaga ggacaatggt ttacaaaaga
     2041 aaccttgcca acattaggtc ttgccaaacc aaccaccttg atactatcca aattaatgga
     2101 gactggcgcc tagcgaagaa ccgttttaaa accaccaaaa ggtgtataaa ctgttaagtc
     2161 cgcataagcc aatatc
//
LOCUS       DAXLDT010000291         2161 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXLDT010000291 DAXLDT010000000
VERSION     DAXLDT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.292"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            81..1808
                     /gene="frgA"
                     /locus_tag="ACF2SP_003117"
     CDS             81..1808
                     /gene="frgA"
                     /locus_tag="ACF2SP_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore biosynthetic protein FrgA"
                     /protein_id="HFD2360865.1"
                     /translation="MALAYGNFHELSHQLRFLLFEIGIGLPQNSVDYFITLAHKNTLN
                     RLQHASIKEGLIQSVIASHHVHDFIDQLQIKLKNSMPESKFFQWRKIREALDESIANE
                     ALAYAYKQNWNTQLRNEAMHYKSLWTWINNELSPYQTLLFLEQWGSLRHPYHPAFSAK
                     TGFTRREVLQNSPEFQAKVSVHWCALNKTKIQSISPKIDYANQISQEFPKEYFCWREK
                     LLFSHINPDDYYPIPVHPWQWRNQLQMAFASLIDNKSLILLPHHQTLIPSLSPDIMMP
                     TQSTQCTLKLATTLSTSMAGKLDNPDDMVLLTRWIDSLLAKTNYYQNTLFICKNLESM
                     SAYDQTLSECNRAKLLFGLYQNPLHKIRQDQRAVPLPALLTDSPCSNTPLLIEIIKAS
                     ELHPTTYFTEYCYKMLFGQLHLLLKYGLALEVEQHNILVIFDDNKPQGIIIKEPNNLK
                     LCNHELFKNVQKPNAPDSLSIYTKDLNQVRTLFIQGTLKNHLHHLISCLCNEYQIPSR
                     TLWGLTRQVMKTVFKDLSKDIDPRILSWQQHLLLHDNWEHQPELLLSLHSKLNRNITI
                     KEYNPLSEI"
BASE COUNT          754 a          441 c          336 g          630 t
ORIGIN      
        1 atttgccccc accccttgca aatgagaatg attatcattt atatttaatt tagaacaaaa
       61 taatccttca ggagataata atggccttgg cgtacggtaa ttttcatgaa ctcagccatc
      121 aattacgctt tttactattt gaaattggca taggactacc acaaaatagt gtggattatt
      181 ttattacctt ggctcataaa aataccctga accgtttaca gcatgcttcc attaaggaag
      241 gattaattca atcggtcatt gcaagtcacc atgtccatga tttcattgac caattgcaga
      301 taaaactgaa aaattcaatg ccggaaagca agttttttca gtggcgaaaa atcagggaag
      361 cattagatga atcgattgct aatgaggctt tggcttacgc ctacaagcaa aactggaaca
      421 cccaattaag aaatgaagcc atgcactaca agagtctgtg gacatggata aataatgaac
      481 tatcaccgta tcaaacgtta ttatttttgg aacaatgggg cagtttgagg catccctatc
      541 acccggcatt cagcgcaaaa acaggattta cgcgaagaga agtactccaa aactctcccg
      601 aattccaggc caaagtcagt gtacattggt gtgcattaaa taaaacaaaa attcagtcaa
      661 taagcccaaa aattgattat gccaaccaaa tttctcaaga atttcccaaa gaatattttt
      721 gttggcgtga aaaattgtta tttagccaca tcaaccctga tgattattat ccaattcctg
      781 ttcacccttg gcagtggagg aatcaattac aaatggcgtt tgcttcttta attgataata
      841 aatccctcat cttgttacct catcaccaaa cactaatacc ttctttatca cctgatatta
      901 tgatgccaac acaatccact caatgtacac ttaaactggc taccacttta agcacctcaa
      961 tggctggaaa actcgataat cctgatgaca tggtattact taccagatgg atcgattccc
     1021 tgttagcaaa aacaaactat taccaaaata ccttgtttat atgtaaaaat ctggagagca
     1081 tgagcgccta tgatcaaact ctctctgaat gcaatcgagc aaaattattg tttggcttat
     1141 atcaaaaccc actccacaaa ataagacagg atcaaagagc agttccatta ccggccttat
     1201 taactgattc accttgtagc aatacaccat tactcattga aattattaaa gctagcgaac
     1261 tgcacccaac aacttatttc actgaatatt gttataagat gttatttgga caattgcatt
     1321 tattgctaaa atatggatta gcactagaag tggagcaaca caatatttta gtcatcttcg
     1381 atgataataa acctcagggg ataattataa aagagccaaa caaccttaag ctatgcaatc
     1441 atgaattgtt taaaaacgtt caaaaaccca acgctccaga ttctctatcc atctatacaa
     1501 aagatcttaa tcaggttaga acccttttca tccagggaac attaaaaaat catctacatc
     1561 acttgattag ctgtttatgt aatgagtatc agattccttc aagaacctta tggggattaa
     1621 ctcgccaagt catgaaaact gtatttaaag acttatccaa agacattgat ccgcgtattc
     1681 taagttggca acaacatcta ttgctccatg acaactggga gcatcaacct gaattgttat
     1741 taagtctgca ttctaaactc aatcgaaata ttacaataaa ggaatacaac ccattatccg
     1801 agatctaagc tctactggac ttacgaaaaa ccccatgcgc tcttgttgca atcttaaaat
     1861 atgagaactt ctcgaggccg ggacgcggat gctatgagtc aataaatccc aattgatttc
     1921 tcaataaggt gacagagaca atggtattgt ataaactaat tcacatacgc catttgaaac
     1981 actcaaataa tagagtattg ttggcacaat atattccctc ctcagcgata aataaaaaaa
     2041 gaggaggaac taattttcgt ataacagatg atactaaaac atattcccaa taccttaaca
     2101 ttgctaagat tagttttaat agcccctttt ttaatgtatt tatttcatca tgaatacgtt
     2161 a
//
LOCUS       DAXLDT010000292         2152 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXLDT010000292 DAXLDT010000000
VERSION     DAXLDT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.293"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            239..1651
                     /locus_tag="ACF2SP_003118"
     CDS             239..1651
                     /locus_tag="ACF2SP_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFD2360866.1"
                     /translation="MNPRSIQLTQNHGGPLHYLGNRYLTLPDLTGHMSPDTSWLNEHL
                     SVLLANNNGQKYKKAIEPFAGSASWSMAAMEIGLAEEYIINDSNKILINTLQLIKDKP
                     DLIKEPYAALVEKYDASLSKKDFFLEVIRNYNQITDEEKALLLPFIINHSWGGILFYD
                     KELNIIYREGELFEGKKADRFLEKANLSLEMFLSEIDRVSILLNANRVTFKSGDFMEV
                     ISIASPGDFVALNPPYPENEHSTLEKAGMYIELYSPEKLHQNLVQIIQHLESQGIHYY
                     MTYGFYNPKFRNYVLTNENQQSINYFRVLGYEDCAFGIGLDQMYFTSQFSIPKGINIF
                     KAEEVLGTQDLTPEEALEHFKLLSKKCFAVIYRAFIKPGLEMEYQKAWHQVASYFVQY
                     RGALGSCLHKTNDGMWLAYSRWPDKATRDASWPGDNAPSEMLPNEIRKAVITIQECID
                     QTQKLPEITMEVVNDLLYSS"
     gene            1670..2107
                     /locus_tag="ACF2SP_003119"
     CDS             1670..2107
                     /locus_tag="ACF2SP_003119"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360867.1"
                     /translation="MNQSQINYQLELVIDTNLLEQQDKIIRDGIVNYNAPFTGTKPER
                     YSVYVKGKEGNIIGGAIVYAHKSSIYVDVLWVKEEYRGLGIGAELLRSVEAEAIKRGI
                     PASTLDTFSFQAEGFYLKQGYQHIGTIKNYLEGHDRIYFRKQL"
BASE COUNT          721 a          365 c          414 g          652 t
ORIGIN      
        1 ggggtagagc tatttttgat ttattcaaat tagaagacgg gaaaatcata gagcattggg
       61 acgcaataca agatattccg gtaaaatcag ctaatccaaa tagtatgttt taatatcaca
      121 ataatgagcc acatcaataa ttaaaaaata agatatgctt gttcaataca ataaaaaatt
      181 tattatttca tgttaagatg tgcagcttta taaataaatt taccacatag tgaccattat
      241 gaatccacgt tctattcaat taactcaaaa tcatggggga ccgctccatt atctcggaaa
      301 taggtacctg actcttccag atttgacagg gcatatgtcc cctgatacta gctggttgaa
      361 tgagcatctc tctgtattat tggcaaataa taatggtcaa aaatacaaga aagcaatcga
      421 accttttgca gggtctgctt cctggagtat ggccgcaatg gagatcggtc ttgcagagga
      481 atatatcatc aatgacagca ataaaattct tattaataca cttcaattaa ttaaagacaa
      541 accagatcta atcaaagagc cctatgcagc attagtagaa aaatatgatg cctcattatc
      601 aaaaaaagat tttttccttg aagtgattag gaattacaac caaataactg atgaagaaaa
      661 agcattactg ctccctttta tcattaatca ttcatggggt ggaatccttt tttatgataa
      721 ggaattaaac attatctacc gtgaaggtga actatttgaa ggaaaaaagg ctgatagatt
      781 tcttgagaaa gcgaacttgt ctttggaaat gtttttaagt gaaattgacc gagtatctat
      841 tctactcaat gctaatcggg ttaccttcaa aagcggggat tttatggaag tgatttcgat
      901 tgcgtcacct ggtgattttg tggcgctgaa tcctccttat ccagagaatg aacattcgac
      961 gcttgaaaaa gctgggatgt atattgaact ttattcgcct gaaaaactgc accagaattt
     1021 ggtgcaaatc attcaacatc ttgaaagcca aggtattcat tactacatga cttatggatt
     1081 ttataatccc aaattcagaa actatgtctt aactaatgaa aatcagcaat caattaatta
     1141 ctttcgtgtt ctaggctatg aggattgtgc tttcggaata ggccttgatc agatgtattt
     1201 tacttcccaa ttttcaattc ccaaaggtat aaacatattc aaagcagagg aggttttggg
     1261 tacccaggat ttaacacctg aagaagcgct tgaacatttt aagctgcttt caaaaaaatg
     1321 ttttgctgtt atttaccgag cctttattaa gcctggactt gagatggagt atcaaaaagc
     1381 ttggcatcag gttgcttcct attttgtgca atatcgcggt gccttaggtt cttgcctgca
     1441 taaaactaac gatggtatgt ggcttgctta ttctcgttgg cccgataaag ctactcgaga
     1501 tgcttcttgg ccaggcgaca atgcaccatc tgagatgtta cccaatgaaa ttagaaaagc
     1561 ggttataacc atccaagagt gtattgatca aacacaaaaa ctacccgaaa ttactatgga
     1621 agtggtcaat gatttattgt attcaagcta attttagaag atataactca tgaatcaatc
     1681 gcaaataaat taccaactag agcttgttat agacaccaat ctactggaac agcaagataa
     1741 aattattcgt gatgggatag taaattataa tgctcctttt acgggaacta aaccagagcg
     1801 ttactcagtc tatgtgaaag gtaaggaggg taatattatt ggcggagcta ttgtgtatgc
     1861 tcataaaagc tcaatatacg ttgatgtcct ctgggttaag gaagaatacc gtggccttgg
     1921 tatcggtgct gaactattac gcagcgttga agccgaagca attaagcgag gtattcctgc
     1981 atcaacatta gatacttttt cttttcaagc agaaggattt tatttaaaac aaggttatca
     2041 gcacataggg acaattaaaa attatttaga aggacatgat cggatctatt ttagaaaaca
     2101 attgtagaaa gagtggggca tatcccccat aagagagtat gccttgaaaa ag
//
LOCUS       DAXLDT010000293         2146 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXLDT010000293 DAXLDT010000000
VERSION     DAXLDT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.294"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            67..405
                     /gene="rpsF"
                     /locus_tag="ACF2SP_003120"
     CDS             67..405
                     /gene="rpsF"
                     /locus_tag="ACF2SP_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFD2360868.1"
                     /translation="MRHYEIMFLVHPDQSEQVPGMVERYEGIITKHNGKIHRKEDLGR
                     RQLAYSINKVHKAHYILMNVECNLDALNEIKNAFKFNDAILRHLITVQKQAITTESVL
                     MKKEKETKVA"
     gene            419..646
                     /gene="rpsR"
                     /locus_tag="ACF2SP_003121"
     CDS             419..646
                     /gene="rpsR"
                     /locus_tag="ACF2SP_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFD2360869.1"
                     /translation="MSAYFRRKKMCRFSAEGGNEIDYKDINLLKNYITETGKIVPSRI
                     TGTQTRFQRQLAKAIKHARFIGLLPYCDSHR"
     gene            600..1559
                     /locus_tag="ACF2SP_003122"
     CDS             600..1559
                     /locus_tag="ACF2SP_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360870.1"
                     /translation="MPDLLVYCLIATAIDKMNLAGNFILRQSRATLESKQYAIVCAVV
                     LSIVPFASWLSVALMSLITLRKGERPGFEVMLPAMIVHSVPLMMFLPLEGALINTLVA
                     YLPCYIAALTLRKTASWPNVFGAFLIQAFLVFLSIEILIPDFVSDQFNHFKVLLSQFQ
                     DYKQLVESSTDGLNNLSLAQLFFGIQILSVIASALISLMFARTIQAKLFMPGGFKQEI
                     LEFRSGRFSFLMLIIVSVASYFEVPVAISTLPLLLAYFFLSGFSLAYYILARKWQVRV
                     VVLLFLLILLKPFFVLFAYIVFGSLDSLFNFRLYLPARVREST"
     gene            1572..2021
                     /gene="rplI"
                     /locus_tag="ACF2SP_003123"
     CDS             1572..2021
                     /gene="rplI"
                     /locus_tag="ACF2SP_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFD2360871.1"
                     /translation="MEVILLEKVRNLGNLGDKVHVKSGYGRNYLIPQNKAVFATEQNI
                     ELFQKRRAELEKKAQQALANAEQRAAKLNDTTIVISAMASDEGKLYGSVGVNEIKDAL
                     IEKQIEISKREIVMPEGPLHSIGNYVVEVHVHSDVVANLQVEIISAK"
BASE COUNT          684 a          339 c          401 g          722 t
ORIGIN      
        1 cagcttttta aaacccttgc cttaccagta ctcaatgctg gaaggcttta tccataagga
       61 gaattcatga gacattatga aattatgttt ctagtacatc cagatcaaag cgaacaagta
      121 cctggtatgg ttgagcgcta tgaagggatt attaccaaac ataacggcaa aatacaccgt
      181 aaagaagatt taggtcgacg ccagctggca tactcaataa ataaggttca taaagcacat
      241 tatatcttaa tgaacgtaga atgtaatctt gacgcactta atgaaatcaa gaatgcattc
      301 aagtttaatg atgctatttt aagacactta atcactgttc aaaaacaagc gattactaca
      361 gaatctgtct taatgaagaa agaaaaagaa actaaagtcg cttaagagga gaacatctat
      421 gtcagcttat tttcgtagaa aaaaaatgtg ccgctttagt gctgaaggtg gtaatgaaat
      481 agattataaa gacatcaacc tattaaaaaa ttatattact gaaactggta aaattgtacc
      541 aagccgtatt actggtactc aaactcgttt ccaaagacaa ttagccaagg caattaaaca
      601 tgccagattt attggtttat tgccttattg cgacagccat agataaaatg aatcttgcag
      661 gtaattttat actcagacaa agtagagcaa ctcttgaaag caaacaatat gctattgttt
      721 gtgcggttgt tctttcaata gttccattcg cctcttggct atcggtggct ttaatgagtt
      781 taattacttt gaggaaaggt gaaagacctg gatttgaagt catgttacct gcgatgattg
      841 ttcattctgt gcctttaatg atgtttttac ctctggaagg ggcattgatc aatacattgg
      901 ttgcttattt gccttgctat atcgctgctt tgactttacg caaaacagcc agctggccaa
      961 atgtatttgg agcttttcta attcaagcat tccttgtatt tctgtccatt gaaatattga
     1021 tacctgactt cgtaagtgat caatttaatc attttaaagt actattgtct caatttcagg
     1081 attacaaaca gttggtagaa agcagtacag atggattgaa taatttaagt ttggctcagt
     1141 tattttttgg tatccagata ttaagtgtga ttgcttcagc cttaatctca ctaatgtttg
     1201 cccgaactat acaagctaag cttttcatgc ccggtggttt taaacaggaa atattagaat
     1261 ttcgtagtgg cagattttca tttttgatgt taattattgt gtctgttgcc tcttattttg
     1321 aggtaccagt agctattagc actttaccac tacttcttgc gtatttcttt ttatcgggtt
     1381 tttctttggc ctattatatt ttagccagaa agtggcaagt tagagtggtg gttcttttat
     1441 ttctattgat cttgttaaaa ccattctttg tcttatttgc ctatattgta tttggctcgc
     1501 tagatagttt atttaatttt cgattatatc tacctgcaag ggtgagagaa tcaacgtaag
     1561 gggttattaa gatggaagta atcttattag aaaaagtacg taatctggga aatctaggtg
     1621 ataaagtaca cgttaaatca ggttatggac gtaattattt gataccacaa aataaggccg
     1681 tctttgcaac agaacaaaat attgaattat ttcaaaaacg tcgtgctgaa ctagagaaaa
     1741 aagcgcaaca ggctttggct aatgcggaac aacgtgctgc taaattgaac gataccacga
     1801 tagttattag tgctatggct agtgacgaag gcaaattgta tggttccgtt ggagttaatg
     1861 aaattaaaga cgcattgata gaaaaacaaa ttgaaatcag caagcgtgaa attgttatgc
     1921 ctgaaggccc tctacattct ataggtaatt atgttgttga agtacatgta catagtgatg
     1981 tagtagctaa ccttcaagtt gaaattatct ctgctaaata atttgtttga tacgacctag
     2041 aaatattcaa atcaacaata tttatataaa ttcttacttt aagttgtgcg tcatgacgca
     2101 caacttgtat tctattattc aatgtgcatt aagatattgt aatttt
//
LOCUS       DAXLDT010000294         2082 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXLDT010000294 DAXLDT010000000
VERSION     DAXLDT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.295"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            112..351
                     /locus_tag="ACF2SP_003124"
     CDS             112..351
                     /locus_tag="ACF2SP_003124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360872.1"
                     /translation="MLDGSIIKVHQDAMRSSYDRSAEAIGSSVGGLSTKIYAKVDSLD
                     QLVNILITPGQVHESQVAHEVWAHESCEFFLADKA"
     gene            complement(583..918)
                     /locus_tag="ACF2SP_003125"
     CDS             complement(583..918)
                     /locus_tag="ACF2SP_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HFD2360873.1"
                     /translation="MDKPLQRQRARDLPKNSTNTGRHLWYHLRAGRLGFKFKRQVPIG
                     AYIVDFVYLEKQLVIELDGSQHMDNKIYDTKRTDWLMTNGFKVLRFWNHDVFQQTPSV
                     LEIITSALL"
     gene            complement(1085..2065)
                     /locus_tag="ACF2SP_003126"
     CDS             complement(1085..2065)
                     /locus_tag="ACF2SP_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFD2360874.1"
                     /translation="MMRRNELLFSDEELIDCLYQGYQIRAVTLYFVNVGSFYGFIVET
                     KENKKYFLKIYPKDQSLVPNPPTIESLHQTGIALNRFKHNFGMTNLSCMLVDVKENYC
                     FTTNGLILTLYDYIEGVHPSYAPNQLLNDKMAALLFQLHRIPSSAFPAFVRENFNIDY
                     ALGMSEWIGHSIEITDTTHARSIFSKLNENKEQLLNGLTQLQQWGKQFSKMSLPFVIT
                     HGDPHHYNVLQTPLDVWLIDWDGIKIAPRERDLWHYLDTPLMKAYSKICPQFTINKNL
                     CKFYRLQRFFEDCRYYLEQVLLGKNTTGMQSEEDKNSFLTHWGWSVCLKG"
BASE COUNT          659 a          382 c          412 g          629 t
ORIGIN      
        1 actatggcaa caggaaaagt gtttataatc gttataataa ctggtcgaaa aaaggttata
       61 tcaccgcaat attggcggaa ttaaaaaaaa gatggcgatc acgaatggca catgcttgat
      121 ggctcgatta taaaagtgca tcaagatgca atgagaagca gctacgatag aagtgcagag
      181 gcaattggct ctagcgttgg tggacttagc acaaaaattt atgccaaagt tgattcattg
      241 gatcaactcg tcaatatact gataacacct ggacaagtac atgagagtca agtggctcat
      301 gaagtttggg cgcatgagtc ttgtgagttc tttttggcag ataaggctta ggatattgat
      361 ggttttcgaa aagcattaac cgatgatggg ataagagctg ttatcccaag caaaaagaat
      421 cgcttgaatc caacgaacca cgatcgtgat atctacaaag aaaggaatat tgttgagcgc
      481 ttttttcagt agattaagcg ttttagacgg attgctacaa gatatgataa aacagccagg
      541 atgtacctca ctggtgtgat gtttgtaagt atattattgg ctttaaagta acgcagatgt
      601 gattatttcg agaacagatg gggtttgctg aaaaacgtca tgattccaaa agcgcaatac
      661 cttaaatcca tttgtcatta gccaatctgt gcgcttggta tcgtaaattt tattgtccat
      721 gtgttgacta ccatcaagct cgataaccaa ctgtttttca aggtagacaa agtcaacgat
      781 atatgcacct attggcacct gtcttttgaa tttaaatcca agtctacctg ctcggagatg
      841 ataccaaaga tgtcgcccgg tattggtact gtttttcggt aaatcgcgag ctcgttgtct
      901 ttgcaaaggt ttgtccattt ccctctcccc aaccctctcc cgcgaaaggc atcatcatac
      961 tattaaagct cctggcgcgg gagagggggg cagatcggag tgaaattgaa aaatccgtgc
     1021 tcaattcaag agtaaatctt gtctaaaaaa acttcaggga cgttgcctag tattgcaaat
     1081 ttatttatcc ttttaggcag actgaccatc cccaatgcgt taaaaatgaa tttttatctt
     1141 cttcagactg cattccagtt gtatttttcc ctaatagcac ctgttcaaga taatatcgac
     1201 aatcttcaaa aaatcgctgt aagcgataaa atttacagag atttttattg attgtgaatt
     1261 gagggcatat ttttgaataa gctttcatta aaggcgtatc cagataatgc caaagatctc
     1321 gttcgcgagg cgctatcttg attccatccc aatcaatcag ccaaacatcc agaggtgttt
     1381 gcaaaacatt gtaatgatgg ggatcaccat gtgtgatcac aaaagggagg ctcattttac
     1441 tgaattgctt accccactgt tgcagctggg ttaaaccatt cagtaattgt tctttattct
     1501 cattgagttt agaaaatatt gatcgagcat gtgttgtatc tgttatttca atagaatgtc
     1561 ctatccattc agacattccc agcgcatagt caatattaaa attttctctc acaaaagcag
     1621 ggaacgcaga tgaggggata cggtgcaact ggaaaagtaa tgcagccatt ttatcattca
     1681 ataactgatt cggtgcatac gaaggatgaa ctccttcgat ataatcatat aaagttaaaa
     1741 ttaatccatt agtcgtaaag caataattct cttttacatc gacaagcatg caagataaat
     1801 tagtcatgcc aaaattgtgc ttaaaccgat ttaaagcgat gcctgtctga tgtagtgatt
     1861 caatcgtggg aggattagga acaagcgact gatctttggg ataaattttt aaaaaatact
     1921 ttttattctc cttggtttca acaatgaatc cataaaacga acctacatta acaaaataaa
     1981 gtgttacagc tctgatctgg tatccttgat ataaacaatc aattagttcc tcatcagaaa
     2041 aaagtagctc attgcgacgc atcattattt ctcattattt tc
//
LOCUS       DAXLDT010000295         2073 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXLDT010000295 DAXLDT010000000
VERSION     DAXLDT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.296"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            111..845
                     /locus_tag="ACF2SP_003127"
     CDS             111..845
                     /locus_tag="ACF2SP_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFD2360875.1"
                     /translation="MEQKSKSIIRVWYLILLLSQYSFANIYVLPEAGNTIGEIQVAFS
                     DYGETIDEVGRRFNVGYYEMVRANPGVDARYPLAANTKLIIPSRFILPNVPRKGVVIN
                     LAEYRLYYFPPDDNVVMTYPVGIGKKGWETPLGLSRITSKEINPTWRPTVKLQKAAES
                     IGAPIPSEFPPGPHNPLGQHVLRLGWPAILIHGSNRVDGIGAKVSAGCIRMLPEDIEH
                     LYSLVSVGTPVRIINAPLQRQADFKQ"
     gene            complement(882..1772)
                     /locus_tag="ACF2SP_003128"
     CDS             complement(882..1772)
                     /locus_tag="ACF2SP_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFD2360876.1"
                     /translation="MQVIPFRYFILLAGTIVSLSLVALVINHFIYHFPGNNYFPPNTP
                     VMACIILLNYIGLILFFGKKNRATSSGLELINFFVVMSIIALATNAVQLTPYPPIDKQ
                     IIKLEAQFGINMISILEWMNKYQHFKYLLAVIYDTLPYQMSIIPLCVIVSGRFYILKD
                     YYFLLLCTTLMGFSYYYFFPTTAPASELQSSLFAPEQIATGLKFHQIHNHIQPTTIEG
                     GLIALPSFHTIWAILCVYLIKDWFIPCLLLSIINVLLILSCVLLGWHYPIDILAAIIL
                     VGLSYYLLTVIKVEARYLDL"
     gene            complement(1783..1980)
                     /locus_tag="ACF2SP_003129"
     CDS             complement(1783..1980)
                     /locus_tag="ACF2SP_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HFD2360877.1"
                     /translation="MDIINLQFEEPLIIRISNTVVKILAFKTQENGNIKFGVEAPRSI
                     NIHREEVFHAIKQKETLSTAD"
BASE COUNT          681 a          347 c          397 g          648 t
ORIGIN      
        1 acatttgaat ctaaatacgg cgaacgtttt aaggtagata ccagcttata atttctctgc
       61 aaggtaagac gttatggttt taccttgctt tttagagaga aaagcatcat atggaacaaa
      121 aatcaaaatc tataataaga gtttggtatt taatactttt actcagtcaa tattcttttg
      181 cgaatattta tgtattgcct gaagcgggaa ataccatcgg tgaaattcag gtcgcatttt
      241 cagactatgg agaaactata gatgaggtcg gccggcgttt taatgtcggt tattatgaaa
      301 tggtacgtgc taacccggga gtggatgcca gatatccgct agccgctaac acaaaattaa
      361 ttattccatc tcgttttatc ctccccaacg tgcccagaaa aggtgttgtg attaatttag
      421 cagaatatcg attatattat tttcctccag atgacaatgt tgtcatgact tatcctgttg
      481 ggattggtaa aaaaggatgg gagactcctt tgggattaag cagaattacc tccaaggaaa
      541 ttaatcctac ctggcgacct acagtaaaat tacaaaaggc agctgaaagc attggtgctc
      601 ctattcccag tgagtttccc cctggccctc acaatccttt agggcaacat gtattgcgtt
      661 tagggtggcc agctatctta atacatggtt ctaatcgagt tgatgggatt ggggccaaag
      721 tcagtgcggg atgtattcgt atgcttcctg aagatattga gcacctatac agtttagtgt
      781 ctgtagggac gccagtaaga attataaatg ctcctctaca aaggcaagct gattttaagc
      841 aataactatt ttcaggtatg tattttcatt cagattaatg cttataaatc caaataacgg
      901 gcctcaactt taattacagt taacagataa taagataacc caacaagtat tatagccgcc
      961 aaaatgtcaa tcggataatg ccatcccagc agtacacatg aaaggattaa tagaacatta
     1021 atgattgaca gcaataggca gggtataaac cagtctttga ttaagtagac acacaaaatt
     1081 gcccagattg tatgaaatga gggaagggct attaaaccac cttcaatggt tgtgggttgt
     1141 atatgattat gaatttggtg aaatttaagt cctgtagcta tttgttcagg ggcaaataaa
     1201 ctgctttgta gttcacttgc aggtgctgta gttgggaaaa agtagtaata agaaaacccc
     1261 attaatgttg tgcagagtaa taggaaataa taatctttaa ggatataaaa tcgccctgat
     1321 acaattacgc ataggggtat gatactcatt tggtatggta gagtatcata tataaccgct
     1381 aataagtatt taaaatgttg atacttattc atccactcca gaatagatat catattaatg
     1441 ccgaattgcg cctccaattt aatgatttgt ttgtctatag gaggataggg agttaattga
     1501 acagcgtttg ttgctaaagc aatgattgac atcacaacaa aaaagttgat caattctaat
     1561 ccagagctag tcgctctatt ttttttaccg aaaaataaaa tcaatccaat ataatttaac
     1621 aagataatgc aagccatgac tggagtatta ggtggaaaat aattattccc ggggaaatga
     1681 tagataaaat gattgataac taaagcaact agcgatagag agactatagt cccagccaat
     1741 aaaataaagt atctgaaagg tataacttgc atttatttgt tgttagtcag ctgtgctaag
     1801 agtttctttt tgcttaattg catggaaaac ctcttctctg tgaatattga ttgacctggg
     1861 tgcttccaca ccaaatttta tatttccatt ttcctgagtt ttaaaagcca aaattttaac
     1921 cacagtattc gagatacgaa tgataagtgg ttcttcaaat tgaagattta ttatatccat
     1981 ttaaaaaaaa cctctttttt cataaattat tcctaaggcc aacattcact gctgaacggt
     2041 accttacttt agtgacagtt gtcactaaaa att
//
LOCUS       DAXLDT010000296         2033 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXLDT010000296 DAXLDT010000000
VERSION     DAXLDT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.297"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..637
                     /locus_tag="ACF2SP_003130"
     CDS             <1..637
                     /locus_tag="ACF2SP_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360878.1"
                     /translation="LTFWAMTFQDILRINEERVTDPYLKKVARHHRLEDAGHEKWFLS
                     DKKYMGEICDNKACDRDDVAWLYSKDSQLVRDAAYSIMSEIYKIEDENLNIVLLLTLE
                     SSGHVFFEKVVKQVKKTGEDKNLKYFSSSHLEVELAHALFEEEMERKVYSRRLPISTR
                     REALRMVDRCYDAFSKMFDGLILACNKRLELAKERNQHVAKSVELRENQAV"
     gene            594..1964
                     /locus_tag="ACF2SP_003131"
     CDS             594..1964
                     /locus_tag="ACF2SP_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFD2360879.1"
                     /translation="MSQNQWSLEKIKQYKEDTGQALLSDEQSLVLFGQDFGKLIQSQP
                     VAVSAPQSTESLQSLILFANQYHLPVTIRGNGLSQGGQSLPVPGGLTISMQSFNKPLD
                     LSEDLIWVEANTSWKNLLEKSLLKNKAPYVLPYNCNLSVGGVLSAGGIGASSFKYGVI
                     NAYVSALEVVDGLGRKQIVEKNSPLFQACLSGQGRFGVITKACIQLRSVQPRVKTFFL
                     VYADQNQWFEDIYKIQGKVDYMEIFCSPSIQGARLKEDKRIPMAYWLYGLHLSVEYDR
                     HAEDILGKLKPWNVLNIQEEGILSYFLRHNSRFDMMKLTGQWDLLHPWYECFVPTSFL
                     TGVLSQLLEELPLHYASLVHVVPIAKQKAGFMMLPDCDSICEFMILNPGVPYSLEESC
                     LKAIENLDKHLLQNNGKRYLSGYLGTELPDNYWLKHFGEKYDSWVGLKKQYDPAGIFS
                     SMLHHI"
BASE COUNT          634 a          333 c          442 g          624 t
ORIGIN      
        1 gttaacattt tgggcaatga cttttcaaga tatacttcga attaatgaag aaagagtaac
       61 tgatccttat ttaaagaaag tagctcgaca tcacagatta gaagatgcag gacacgaaaa
      121 atggttttta agtgataaaa aatacatggg tgaaatatgt gataacaagg cctgtgatcg
      181 cgatgatgtt gcctggctat acagtaagga ttcccaattg gtacgtgatg ctgcctattc
      241 tattatgtct gaaatttata aaatagagga tgagaatctg aatattgtct tgctgttgac
      301 cctggaatca tcaggacatg tattttttga gaaagttgtc aaacaagtta aaaaaacggg
      361 tgaagataaa aatctgaaat acttctcaag ttctcatctt gaagtagaat tagcacatgc
      421 tttatttgaa gaggaaatgg aaagaaaagt atattccaga agattaccaa ttagcactcg
      481 tagagaagca ctaaggatgg tagatcgttg ctatgatgca ttcagcaaga tgtttgatgg
      541 attgattctt gcttgcaata aacgattgga attggcaaaa gaaaggaatc aacatgtcgc
      601 aaaatcagtg gagcttagag aaaatcaagc agtataagga agataccgga caagcattgc
      661 tcagtgatga gcaatcgctt gttttatttg gtcaggattt tggcaagctc attcaatcac
      721 agccggtagc tgtatccgcc cctcaaagca cagagtcatt gcaatcatta attctattcg
      781 ccaatcaata tcacttacca gtgactattc gtggtaatgg tttaagtcaa ggtggccaat
      841 cattgcctgt tcctggagga ctaacaattt ctatgcagtc tttcaacaag ccacttgatt
      901 tgagtgaaga tctcatctgg gttgaggcca ataccagttg gaagaatctg cttgaaaaat
      961 cactactgaa gaataaagcc ccctatgtgc tgccttataa ctgtaatctg tcggttgggg
     1021 gtgttttatc tgcaggagga ataggggctt cctcttttaa atacggtgtt attaatgctt
     1081 atgtttcagc attggaagtg gttgatggtt tggggagaaa gcaaatcgtt gaaaaaaact
     1141 cacctctatt tcaagcctgt ctttcagggc aaggtcgatt tggtgtcatt accaaggcat
     1201 gtattcagct acgatcagtt cagccacgcg tcaaaacttt ctttctggtt tatgctgatc
     1261 agaatcaatg gtttgaagat atttataaaa tacagggtaa agtcgattat atggagatat
     1321 tttgttctcc ttcaattcag ggagctcgat tgaaagaaga taaacgtatt cccatggctt
     1381 attggttgta tggtttgcac ctttctgttg aatatgatcg gcatgcagaa gatattttag
     1441 ggaagttaaa gccctggaat gtcctaaaca tacaggaaga gggcatttta tcttattttt
     1501 taaggcataa ctctcgcttt gatatgatga agttgacggg gcaatgggat ttgttacatc
     1561 cttggtatga atgttttgtt cctacctcat ttttaacagg agtactttcc caactgttag
     1621 aggaattacc acttcactat gccagcttgg ttcatgttgt tccaattgcc aagcaaaaag
     1681 ccgggtttat gatgttacca gactgcgatt caatttgtga atttatgatt ttaaatccag
     1741 gggtacctta ttccctagag gaaagttgcc tgaaggctat tgagaatctc gataagcact
     1801 tgctgcaaaa taatggtaaa cgctatttat caggatactt aggtacagaa ttaccagaca
     1861 actattggct aaagcatttt ggagaaaaat acgactcatg ggtaggtttg aaaaaacagt
     1921 atgatccagc agggattttt tcatcgatgt tacatcatat ataaaaaata gtattgtgca
     1981 aactcatgct tcgtaacaga tatcaaggca tggtggtcga tctgatgcat aaa
//
LOCUS       DAXLDT010000297         2024 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXLDT010000297 DAXLDT010000000
VERSION     DAXLDT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.298"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(64..858)
                     /gene="istB"
                     /locus_tag="ACF2SP_003132"
     CDS             complement(64..858)
                     /gene="istB"
                     /locus_tag="ACF2SP_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISLpn12 family helper ATPase
                     IstB"
                     /protein_id="HFD2360880.1"
                     /translation="MNIQHERILSLCERLNLSAIATNYSYLAQEAATHNQGFSDFLEN
                     VLTVELQEKQYRSRTLLTKMAGFPMIKTIDDFDFKFASGVPKNKIRELTSLAFIERQE
                     NILLLGPSGLGKTHLAIALGYLAVQANIKTKFISAADLMLLLETAHRQGRYKDIMRRV
                     INNARLLIIDEIGYLPMNRDQANHFFQVIANRYEKGSIIATSNLAFGQWDNTFAGDKV
                     LTAAMLDRLLHHAHVIQCRGDSYRLKDKFKAGLIQNQKEEQNNITD"
     gene            complement(855..1877)
                     /gene="istA"
                     /locus_tag="ACF2SP_003133"
     CDS             complement(855..1877)
                     /gene="istA"
                     /locus_tag="ACF2SP_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISLpn12 family transposase"
                     /protein_id="HFD2360881.1"
                     /translation="MIHILYKQGYSKKAIARKLGMSINTVRKYLKSGSEPYYKRRDKK
                     PLKLDPYKAYIQKRLKDATPHWVPATVIYREIKLFGYDGCSSQLRAYMHTLRSKKEDV
                     IVRFETEPGQQMQVDWAQFRRGKDRLSAFIATMGYSRASYVEFVSDETLETLIACHKN
                     AFEYFGGVPYTILYDNMKTVIIERNGYGLSQHRFQAGFWDFAKHTGFRPKVCQPYRAQ
                     TKGKVERFIHYLKYSFYFPLVGQLKALGLSLDKETANMHVLKWLNEIANQRVHATTGA
                     IPFERLLDEQAKLQPLSSTYSGKLFSHLENKEVHYFAFQLLDNTAMQHELSIYQKLLE
                     RTEEAA"
BASE COUNT          616 a          397 c          335 g          676 t
ORIGIN      
        1 gtcaatgccg attgaaaatt gatccttttg ccagatgaaa attgatcccc cctggtaaaa
       61 aaattaatca gttatattat tttgctcctc cttctgattt tgaattaaac cggccttaaa
      121 tttgtctttt aatctataac tgtcgcctct gcattgaatg acatgagcat gatggagcaa
      181 cctgtcgagc atagccgctg ttaatacttt atcgccggcg aaagtattgt cccattgacc
      241 aaaagcgaga ttggaggtgg caataataga acctttttca tagcgatttg caataacttg
      301 gaagaaatga tttgcctgat ctcggttcat tggtaaatat ccaatttcat caataatgag
      361 cagtcgagca ttattaataa cccgtcgcat aatatcttta tatcgccctt ggcgatgagc
      421 tgtttcaagc aataacatca aatctgctgc gctaataaat tttgttttaa tgtttgcttg
      481 aactgctaaa taacccagag caatcgctaa atgagtcttg cctaatccac taggaccaag
      541 taataaaata ttttcctgtc gttcaatgaa agccaaagaa gtcagttctc ggattttatt
      601 ttttggtaca ccagaagcaa atttaaaatc aaaatcatct attgttttaa tcattggaaa
      661 accagccatt ttagtgagca gggttcgact tcgatactgt ttttcctgta actccacagt
      721 caatacattt tctaaaaaat cactaaatcc ttgattatga gttgctgcct cttgagctaa
      781 ataagagtaa tttgtcgcta tcgctgataa attcaagcgt tcacatagtg ataaaatacg
      841 ctcatgttga atattcatgc agcctcctct gtgcgctcga gtagtttctg ataaattgac
      901 aattcatgtt gcattgcagt gttgtctaat aattgaaagg caaaatagtg aacctcctta
      961 ttttccaaat gagaaaataa tttaccagaa taggtagatg acaatggttg tagtttcgct
     1021 tgttcatcaa gaagtcgttc gaatggaatt gctcctgttg tagcatgtac tcgttgatta
     1081 gcaatttcat ttagccattt taaaacatgc atattggctg tctctttatc taaacttaag
     1141 ccaagtgcct tcaattgccc aactaaagga aaataaaaac tgtactttaa ataatgaata
     1201 aagcgttcaa ctttaccttt tgtttgtgcc ctataaggtt gacacacttt aggacgaaat
     1261 ccagtatgtt ttgcaaaatc ccaaaaacca gcttgaaatc gatgttgact taggccatat
     1321 ccatttcgtt caataatgac tgttttcata ttgtcatata ggatcgtata aggaactcca
     1381 ccaaaatatt cgaatgcatt tttatggcat gcaataagcg tttccaaggt ttcatcgctg
     1441 acaaactcta cataagatgc tctggaatat cccatggttg caataaaagc acttaatcta
     1501 tcctttcctc ttcggaattg agcccaatcc acctgcatct gctgtccagg ctctgtttca
     1561 aaccgaacaa taacatcttc tttttttgac cttaaagtat gcatatatgc tcttaattgg
     1621 gagctgcatc catcataacc aaacaattta atctctcgat aaatcacagt agctggtacc
     1681 caatgaggtg ttgcatcttt taatcttttt tgaatatatg ctttataggg gtcaagcttt
     1741 aatggctttt tatcacgcct tttgtaataa ggctctgaac cacttttaag atattttcga
     1801 acagtattta tcgacatacc caattttcta gcaatcgctt ttttactgta tccctgctta
     1861 tacaaaatat gaatcatttt tgactcctct aattgaatca ttccctcctc cagaatttat
     1921 tctcctgaag aaaactatag tagggggatc aattttcagt cggcaaaggg gatcagttta
     1981 tattcagcaa cgacatttaa tcggaaagta ggttatgcta caac
//
LOCUS       DAXLDT010000298         2015 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXLDT010000298 DAXLDT010000000
VERSION     DAXLDT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.299"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1480
                     /gene="vpdA"
                     /locus_tag="ACF2SP_003134"
     CDS             <1..1480
                     /gene="vpdA"
                     /locus_tag="ACF2SP_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VdpA"
                     /protein_id="HFD2360882.1"
                     /translation="TRFAQFQNFLEENIYSCPENITFQTLARIKEICPECELGEKLFI
                     TGTNLSTQKHEVFSIDTTPSMALADAIIISANLPIAFERICYQGNVYSDGGISNNLPA
                     HCFSEKGLKTTFLKHKDDVDFSVLALQFDNGLEENALYSQNPIPKWSWLSNTFYSLIT
                     GHPNVTENWHEDLQILRRHAHQSILIKTPTIALTNLTISQDTKKALVESGRTAAKNYL
                     DLHEFCMDDYGNIRHNECLHEKFQKPEELLDYCVLHSHFELLKKIKQAISCSQYLEKG
                     YKHYLCELCDNLLPPQPKCPNEGSGTEQSEIKLEKDTIICEKKNNSGLTFFGVPSPLV
                     KTLNKDSPELKIKLFTGLYPILIQNWQNLCPVSGISGILNSIRMSFVEIRSTDTCIKT
                     LIDKLNEIEIGHFLIFVFKAALKNYDQHDFILLLKNLKHLHHSIELIRNKPFHSDDRF
                     YGQWSFEGHDPKRILEFIKSDDISGLMTILEDKKALPNNKPN"
     gene            complement(1583..>2015)
                     /locus_tag="ACF2SP_003135"
     CDS             complement(1583..>2015)
                     /locus_tag="ACF2SP_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360883.1"
                     /translation="LFCNSRSVPWVYQLSFVFYDYILDKEFEFITTKSGRDIFDAKKQ
                     LVLKYIQKAADLYPRYEKEDFSDCHEMVNILLLSMRSEENNLQKRRNQDSSTSYMYSY
                     FTQTLYNASGKLMGKGQLGQKIDLLIEEFNERVVNKTPLYT"
BASE COUNT          698 a          375 c          326 g          616 t
ORIGIN      
        1 aacacgattt gcacaatttc aaaatttttt agaagaaaat atatacagct gccccgagaa
       61 tataacattt caaaccttag ccagaataaa agaaatatgc cctgaatgtg aattaggaga
      121 aaaactattt attactggaa caaatttgag tacccaaaaa catgaagtgt tttccattga
      181 tacaacacca tctatggcac tcgccgatgc cattattatt tccgcaaatt tacctattgc
      241 ctttgagcgt atctgctatc aaggaaacgt atacagcgat ggaggaatca gcaataattt
      301 acctgcccat tgtttttcag aaaaagggct taaaacaacc tttttgaaac acaaggatga
      361 tgttgacttc agtgtacttg ctctgcagtt tgataatggg cttgaagaga acgctcttta
      421 tagccagaac ccaataccta aatggtcatg gctaagcaac acattctact cattaattac
      481 aggacatcca aatgttactg aaaactggca tgaggatctt caaatactga ggcgacatgc
      541 ccatcaaagt attttaatta aaacgccaac tattgctttg accaatctca caattagcca
      601 agatacaaaa aaagccctgg ttgaatcagg gcgaacagct gccaagaact atctggatct
      661 ccatgagttt tgtatggatg attatggaaa tatcagacat aatgaatgct tgcatgaaaa
      721 atttcaaaaa cccgaagagt tattggatta ttgtgtccta cacagccact ttgaactttt
      781 aaaaaaaata aagcaagcca tttcctgctc acaatattta gaaaaagggt ataaacatta
      841 tttatgtgag ttgtgtgaca acttgctccc acctcaaccg aaatgcccca acgaaggttc
      901 aggaacagaa caatctgaaa tcaagttaga aaaagatacc atcatttgcg aaaagaagaa
      961 taatagtggg ctgacatttt ttggggttcc atccccttta gtcaaaacat taaataagga
     1021 tagccccgaa ttaaaaatta aactatttac aggtttatat cctatactaa ttcaaaactg
     1081 gcaaaattta tgccccgttt ccggcattag tggtatatta aattctatcc gcatgtcatt
     1141 tgttgaaatc cgttcaacag atacctgtat aaaaacatta attgataaac tgaacgaaat
     1201 tgaaattgga cattttttga tatttgtttt taaagcggct ttaaaaaatt acgatcaaca
     1261 tgacttcata ttactattga aaaacctgaa gcatcttcat cattctatcg agctaatcag
     1321 aaataagccg tttcattctg acgaccgatt ttatggacag tggagttttg aagggcatga
     1381 tcccaaaaga atcctcgaat tcattaaaag cgatgatatt tcaggattaa tgactatttt
     1441 agaagataaa aaggcgcttc ccaataataa accgaattaa caggtttaaa tccaacgcgt
     1501 cactgagtta acaaatcaac ttgaattggt gtttttacta ttaatccagt gacttttctg
     1561 tcttgctttg gaaaataaag aattaagtat acaagggtgt tttattgact actctttcat
     1621 taaattcttc tatcaataaa tctatttttt gccctaattg ccccttcccc attaatttcc
     1681 cggaagcatt ataaagtgtt tgtgtaaaat aagagtacat ataacttgta ctactatcct
     1741 gatttctgcg cttttgtaaa ttattttctt cagaacgcat gctgagtaac aggatattca
     1801 ccatctcatg gcaatcagag aaatcttctt tttcatagcg aggataaaga tcagctgcct
     1861 tttggatgta tttaagaacc aactgtttct ttgcatcaaa aatatctctg ccgcttttag
     1921 tggttataaa ctcaaattcc ttatcaagga tatagtcata gaatacaaag gaaagctgat
     1981 acacccaggg tacgcttcta ctattgcaaa acaat
//
LOCUS       DAXLDT010000299         2014 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXLDT010000299 DAXLDT010000000
VERSION     DAXLDT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.300"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1018)
                     /locus_tag="ACF2SP_003136"
     CDS             complement(<1..1018)
                     /locus_tag="ACF2SP_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFD2360884.1"
                     /translation="MLFRHKGILYLFLSSLIISAANPVIAKLITLGNEHLVNGRNPIS
                     FCNVLFAGNFIAVLTLILIHFRELKEFQPEHLTAKNWCLIFLAALFAGFLTPTLFFFG
                     LMYTNVINVVLISNLQIPMTLLAGWIFLGEVPNIRVTTGAFLATLGVFAIVILQYWLG
                     GAGALTPNPGMKTGPLFIFLSSIPRSGEICIFLAVVSSTASTIIGFHALRRLPHYLFS
                     ILKMLLGLVLFFFIVISMFGWHHFADLFSPFLWEWMLFYGSVIIALRYYFSMLGMKHA
                     KVAEVAISSSIIPLMSIIFSYLILGEVPGISQIIGGSLILFGIYVALTGKLQAPEKTE
                     VLEKP"
     gene            complement(1387..>2014)
                     /locus_tag="ACF2SP_003137"
     CDS             complement(1387..>2014)
                     /locus_tag="ACF2SP_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg1158 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360885.1"
                     /translation="EDARELFKSLKEILPEEARLKVRESKQEPDQFRVVLFNPEKVFS
                     FYAEHAAELLNRFKPLPTSSSGEEYCKWKYNPLSHLIEYTVPFSILDSDTKAKVTDTH
                     EEAIQSKVNQYNEKLRSIYPDLKIIGTNSVEDSSPDKFFLGSFNYNDYKIIKNLNNPA
                     SLRQLAIGFFKEHPGHYTEEVAKKIPGDMMDEFTDIEPSSTKPSSTGS"
BASE COUNT          727 a          376 c          317 g          594 t
ORIGIN      
        1 aaggcttttc caagacttcg gttttctcag gagcctgcaa ttttcctgtt aaagcaacgt
       61 atatcccgaa caaaattaaa cttccaccta tgatttgcga aataccggga acttcaccta
      121 atatcaaata ggaaaaaata atagacatca gaggaattat tgatgaactg attgcaacct
      181 ctgcaacttt tgcatgtttc atgcccagca tactgaaata atatcgcaaa gcaataataa
      241 cactcccata aaagagcatc cactcccata aaaatgggga aaaaagatca gcaaaatgat
      301 gccagccaaa catagaaatc actataaaaa agaataaaac aagccccaat aacattttta
      361 aaatactaaa cagataatgg ggtaatcttc taagtgcatg aaaaccaata atagttgaag
      421 cagtactgga aactactgcc aaaaaaatac aaatttcacc tgaacgagga atagagctga
      481 gaaaaataaa aagaggacct gttttcattc cagggtttgg agttaaagca cctgctcccc
      541 ccaaccaata ttgaagaata actattgcaa atactcccaa ggtagctaaa aaagcccctg
      601 tggttactcg aatattaggc acctctccca agaaaatcca gccggctaat aaagtcatag
      661 ggatctgtag attggaaata agaacaacat taatcacatt ggtgtacatt aatccaaaaa
      721 aaaataaggt gggcgttaaa aaacctgcga ataaagcagc cagaaaaatt aaacaccaat
      781 ttttcgctgt taaatgttct ggttgaaatt cttttagctc tctaaaatga attaaaatca
      841 aagtgagcac tgcaatgaag tttccggcaa ataagacatt acaaaacgaa attggattac
      901 gaccattgac caaatgctca ttcccaagag tgataagttt tgctatgaca gggttagcag
      961 cacttattat gagcgaactc aggaataaat atagtattcc cttatgtcga aacagcatct
     1021 taattttccc cctatatcct tatcaaggct ttaattttgt acactcaatt ttacctgtat
     1081 ctaaatcttt tttcaggtga gaaacagcaa taaatagcat atcttgatta gcccacatga
     1141 tgaacaatca aacaacatgg atagaatacg cacgattttg acaatcttat ctttcgaatc
     1201 aatacataaa atttctttag attccttaag tgcagctata aatttacttt ataaatctcc
     1261 cttacaacac aatacgctgc aataagagaa gagaaaagga aatgatacaa ttaaatccac
     1321 gtttatttac aagataaaaa gccacagttt ctatttatcc gttactcgtc tatgctcaat
     1381 aaaaaattag ctgccggttg acgatggttt agtggaggat ggttcaatat cagtaaactc
     1441 atccatcata tctcctggta tttttttggc aacttcttct gtataatgac caggatgttc
     1501 tttaaaaaat ccaattgcta attgacgcaa acttgcagga ttattaagat tcttaatgat
     1561 tttataatca ttataattaa aagatcctaa gaaaaattta tcaggactag agtcttcaac
     1621 tgaattagtt ccgataattt tgagatcagg gtaaatcgac cgtaacttct cgttatattg
     1681 atttactttt gattgtatag cctcttcatg agtatctgta actttcgctt ttgtatcaga
     1741 atctagaatt gaaaacggaa cagtatattc tattagatgt gataaaggat tatatttcca
     1801 tttacaatat tcctctcctg aagaagatgt cggtaatggt ttaaatcgat ttaacagttc
     1861 tgcagcatgc tcggcataaa acgaaaaaac cttttcagga ttaaagagaa ctacacgaaa
     1921 ctgatctggt tcttgctttg actcacgtac ctttaatcgc gcctcttcag ggagtatttc
     1981 ttttaatgat ttaaataatt cccgggcatc ctct
//
LOCUS       DAXLDT010000300         2014 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXLDT010000300 DAXLDT010000000
VERSION     DAXLDT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.301"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            104..1324
                     /locus_tag="ACF2SP_003138"
     CDS             104..1324
                     /locus_tag="ACF2SP_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2360886.1"
                     /translation="MKFTDTYIRNLKPQQKWFEQIEFSGLGIRVMPGGGKSWIFRFTF
                     DGKRYKMTLGKYPGIGLKEARELMLDAEHLKEQGINPIEYAKQQQAKSDNTVKKLAFS
                     WYTQYVEKHLKRPLTVKKQIDGDIINLLGDRVLDELETKDITEALDKIVKRGASVHAN
                     RVLSSLKQMFGYAVSRGTMSTNPASNIRARDIGGHEKPRERVLSLDEIKSLWLFLDSK
                     DSQMAPQTRIAIKIILLTGVRTAELRLAQWSEINFDDSLWTIPAMHSKASIVHKVHLS
                     DLTKQLLQQLKSISRSDYVLTGADYRQPLTENALPRAIKRIQERLGIPEWTAHDLRRT
                     FATQLGETLQVDPVVIEKCLGHKMPRIMATYNCNEMLPQRREALERWAVCIENMMQDN
                     VVYINKLLQNERSL"
     gene            1408..1731
                     /locus_tag="ACF2SP_003139"
     CDS             1408..1731
                     /locus_tag="ACF2SP_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFD2360887.1"
                     /translation="MSWIVEFYNESVEEAILTMPPKIQARMIKLLELIETHGANLGPP
                     HTEAMGDGLFEIRAKAQEGIGRSLYCYMKGKHIVVLHAFVKKSAKTPKPDLQLALKRK
                     REVEK"
     gene            1728..2012
                     /locus_tag="ACF2SP_003140"
     CDS             1728..2012
                     /locus_tag="ACF2SP_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2360888.1"
                     /translation="MMKLKTLKAKALKNPEVKEAYDALESEFALIDALLTMRQKAGLT
                     QEELAERMGTKKGNISRLEKGNTNPSWNTLEKYAHACGFDISMAVKRIAS"
BASE COUNT          681 a          352 c          436 g          545 t
ORIGIN      
        1 agtttttatt gaaatttgac tttattttaa tatcatttaa tattatctga attcacattt
       61 agtggtacca aaaacggtac caaaaatcat cttggtacca gttatgaaat ttaccgatac
      121 ttacatccgc aatttaaaac cccagcaaaa atggtttgaa caaatcgagt tcagtggcct
      181 tggaattcgg gtcatgccag ggggaggtaa atcatggatc tttcggttca cttttgacgg
      241 caagcgttac aagatgactt tgggtaagta cccggggatt ggcttgaagg aagcacgtga
      301 gctgatgctg gatgcggaac atttaaaaga acaagggatt aatcccatag agtatgctaa
      361 acagcaacaa gccaaatcag ataatacggt aaaaaagctg gcatttagct ggtataccca
      421 atatgttgaa aagcacctaa agcgtccctt aacggttaaa aagcaaattg atggtgacat
      481 cattaatctg cttggtgatc gggtattaga tgaactggaa accaaagaca ttacagaagc
      541 cttggataaa atagtcaaac gaggggcaag cgtacacgca aaccgcgttt taagctcttt
      601 gaagcaaatg tttggctatg cggtaagccg tggcactatg agcactaatc cagcctctaa
      661 tattcgcgca agagatattg gcggtcatga gaaaccaaga gagcgtgttt taagtctgga
      721 tgaaatcaaa tccctgtggt tatttctgga cagtaaagac tctcaaatgg caccacaaac
      781 ccgtattgct attaagatta ttttactaac tggtgttcgt actgctgaac tgcgcttggc
      841 gcaatggagc gaaatcaact ttgatgactc cctttggaca ataccggcta tgcattccaa
      901 ggctagtatt gttcataaag tacatcttag cgatttgacc aaacagttat tgcagcaact
      961 caaatccatt agtcgatcag attatgtctt aacaggtgca gattatcgcc aaccattgac
     1021 agaaaatgct ttgccaaggg ctattaaacg cattcaagag cgtttaggta ttccagaatg
     1081 gacagcccat gatctacgcc gtacttttgc cacccaacta ggagagacgc tccaagttga
     1141 tccagttgtc attgagaaat gccttggcca taaaatgcca cggattatgg caacttataa
     1201 ctgcaatgaa atgttacctc agagacgaga agctctcgag cgttgggcgg tatgtattga
     1261 aaatatgatg caagataatg tggtatatat taacaaacta ctccaaaacg aaagatcttt
     1321 gtaaaacaaa tttgtcattt ttatttgcga ctggaaaaaa taataacaat aagttatcat
     1381 ataagataac aaaggattta ataataaatg tcttggattg ttgaatttta taatgagtct
     1441 gttgaagagg ccatacttac aatgccgcca aaaattcagg ctcgaatgat taaacttttg
     1501 gaattgattg aaacacatgg cgctaatctt gggcctcctc atacagaagc gatgggtgat
     1561 gggttatttg agattcgcgc aaaagctcaa gaaggaatcg ggcgatcgct ttattgctat
     1621 atgaagggaa agcatattgt ggttttgcat gcatttgtaa agaaaagtgc aaaaactcca
     1681 aaaccagatt tgcaattagc tctcaaaaga aagcgggagg tagaaaaatg atgaaactta
     1741 aaacattaaa ggctaaagcg ttaaaaaatc ctgaagtaaa agaagcatac gatgctttgg
     1801 aatccgaatt tgcattgatc gatgcgttac tgacgatgcg tcaaaaagct ggattaactc
     1861 aagaagagtt agctgagcga atggggacaa aaaaagggaa tatttctcga ctggaaaaag
     1921 gaaatactaa tccaagctgg aatactttag agaaatatgc acatgcgtgt gggtttgata
     1981 tttcaatggc agtaaagaga atcgcttcat aatt
//
LOCUS       DAXLDT010000301         2001 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXLDT010000301 DAXLDT010000000
VERSION     DAXLDT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.302"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..544
                     /locus_tag="ACF2SP_003141"
     CDS             <1..544
                     /locus_tag="ACF2SP_003141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360889.1"
                     /translation="VVRKVIISNFIEEINKLVVVEETPILSIFLKKASDIKPFQCSVN
                     TINLRKTILNKVPYSNELEKNFLIFLDHVNDVEAFIKNETRTMNLKIPYVDHAGFLRN
                     YIPDFVVKTPDLMLIVETKGRIDIDVAAKDAQTRRWAQMVSAKTNKNWEFLRVNQSDF
                     DGGGYNSIRDFLILASKQKH"
     gene            719..1253
                     /locus_tag="ACF2SP_003142"
                     /pseudo
     CDS             719..1253
                     /locus_tag="ACF2SP_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1395..>2001
                     /locus_tag="ACF2SP_003143"
     CDS             1395..>2001
                     /locus_tag="ACF2SP_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2360890.1"
                     /translation="MVRPLRIEYAGGLYHITSRGNRKEAIYLSDEDRENFLSVLGDVC
                     LKYQWLCHSYCLMTNHYHLFIETPLGNLSKGMRYLNGVYTQKFNKSHKRIGHVLQGRY
                     KSILVEKDSYLLELSRYIVLNPVRARMVEKADEWHWSSYLATSGRAPVPSWLTVDWLL
                     SSFGSIKSEALINYEQFVDAGLIKKNPLLDLKHQIYLGSDEF"
BASE COUNT          657 a          317 c          423 g          604 t
ORIGIN      
        1 cgttgttcga aaggtaatta tttcaaattt tattgaagaa ataaacaaac tggtggttgt
       61 agaggaaacc cctatattaa gcatattctt gaagaaagct tctgatatta agccttttca
      121 atgttcagtt aatacaatca atttgagaaa aacaatcttg aataaagttc catactccaa
      181 tgagctagaa aagaattttt taatattctt agatcatgtt aatgatgtag aggcatttat
      241 taaaaatgaa actagaacaa tgaatttaaa aattccatat gtagatcatg ctggattctt
      301 gcgtaattac ataccagatt ttgttgtgaa aacccctgat ttaatgctta tcgttgaaac
      361 taaaggacga atagatatag atgtagcagc caaagatgct caaactaggc gttgggctca
      421 aatggtaagc gcgaagacta acaaaaactg ggaattttta agagttaacc aaagtgattt
      481 tgatggaggt gggtataatt ctattcgtga ttttttgata ttagcatcca aacaaaagca
      541 ttaatttgat gctacacaaa aactttccgg tactgatgta gcaataatac tctggactcc
      601 tcaatgttat tattcttaat atattcatga aataaattga atagtgtaac cctcccctaa
      661 aataggtgca tttctgatta gagttctcca agttaaacta tgtttgagga gaacaaaaat
      721 gaaaaaatcg cgttatacag aaacacaaat tgtaaaaatt ctaaaagaag tcgaagcagg
      781 tcggctggtt aaagaaattt gtcgtgaata tgggatttca gatgcaacct attacaactg
      841 gaaagcaaaa tatggtggca tggaggcatc cgacgtaaaa cgtctgaaag atcttgaaga
      901 agagaaccgt cgtctaaagc aaatgtatgc tgagctaagt cttgatcaca aaatcctgaa
      961 ggatattgtc gaaaaaaagc tatgaagcca tctgtgagac gggagctggt tgattatgca
     1021 gtgaatactc atactgtgag tttacgccgt gcttgtaagg ttgtgggtat cagcgactca
     1081 gtttatcgat ataatataag ccggatagcc aatccgatga aggcgttatt gtggcattgc
     1141 aggaatcatc cgaacgctat ccggcgtatg gctttagtaa attgcttaaa gtcttgcgcc
     1201 gtcagggtca taggtggaat cataaacgcg tttatcgggt atattgtgag ctgtagcctt
     1261 ccgcacgtac agggtcagat cttgcttttt gcctttatcc ataaaagacg gcaagcttgg
     1321 aattaccgct tgataattat ttcccaatgc attaattttc tttcaaaagg aattaagcaa
     1381 gtccaggaac agcgatggta agacctttgc gtattgagta tgctggtggg ctctatcaca
     1441 tcaccagtcg agggaataga aaggaagcga tctatttatc agatgaagac agagagaatt
     1501 ttttatctgt tttgggtgat gtttgtttaa aatatcagtg gttatgccat tcgtattgtc
     1561 tgatgacaaa tcattaccat ttattcattg aaactccgct cggtaatctt tcaaaaggaa
     1621 tgcgctatct gaacggggta tacactcaga aatttaataa atcacacaag cgaattgggc
     1681 acgtcctgca aggtcgctat aaatctatac tggttgagaa agacagctat ttattggagt
     1741 tgtcgcgtta catagtatta aatccggtta gggcaaggat ggttgaaaag gcagatgagt
     1801 ggcattggag tagttactta gcaacaagtg gtagagcacc agttccatca tggctgacag
     1861 ttgactggct tttatcatca tttggttcta ttaaatcaga ggcactaata aactatgaac
     1921 aattcgttga tgctggttta attaagaaaa atccgttgct tgatctaaag catcaaatat
     1981 atcttggctc ggatgagttt a
//
LOCUS       DAXLDT010000302         1930 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXLDT010000302 DAXLDT010000000
VERSION     DAXLDT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.303"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..622)
                     /locus_tag="ACF2SP_003144"
     CDS             complement(<1..622)
                     /locus_tag="ACF2SP_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360891.1"
                     /translation="MTKQKWKELHSSHHREKKDSYIEYFKEHLIEHLTKEHDPNGETN
                     QPGKIIEASRSTTGKLSRIFDAYHFPAPNYEELNAIHQKPNGLREHMQENLNGIIPVL
                     LNEDRTALHPKVIEAIGRDNYEAILKNAQGNEQEIALQFLKAAIISYGQRMLDNTDDS
                     NLKNKAFISIMPALEKLASEVTLKGLPEQSKETNPLDILKMSQDLLK"
     gene            complement(855..1232)
                     /locus_tag="ACF2SP_003145"
     CDS             complement(855..1232)
                     /locus_tag="ACF2SP_003145"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HFD2360892.1"
                     /translation="MSKTYTEKLFSYGTLRYEKVQIANFGRKMQGQEDVLQGFKLTTI
                     EIKNSAVVATSGDNFHPIISYTGNPSDKVPGIVFTISKEELAQADKYEVSEYKRVNVK
                     LNSGVNAWVYINAQDLYGNNQTK"
     gene            1321..1614
                     /locus_tag="ACF2SP_003146"
     CDS             1321..1614
                     /locus_tag="ACF2SP_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFD2360893.1"
                     /translation="MMSKFFNLKTGTLHSVESYAGAVKLPKDISQESIRINERILDND
                     GYPLMAPKLRRQNANQLRMFFSRESNTLLNGSYDLADRSNRDLTEVANIISYS"
BASE COUNT          585 a          338 c          314 g          693 t
ORIGIN      
        1 atttcaagag atcttgtgac atttttagaa tatcgagtgg gtttgtttct ttagattgtt
       61 caggcaaacc tttcaaagtt acttcactgg cgagtttttc cagggcaggc ataattgaaa
      121 taaaagcttt atttttcaga ttactatcat cagtattatc tagcatcctt tgcccatagc
      181 ttattattgc tgcctttaag aattgcagag caatctcctg ttcattaccc tgagcatttt
      241 ttaaaattgc ttcataatta tctcgcccta tcgcttcaat aacctttgga tgaagtgcgg
      301 ttctatcttc attaagtaaa acagggataa tcccatttag attttcctgc atgtgctctc
      361 ttaatccatt aggtttttga tgaatagcgt ttaactcttc atagtttgga gccgggaaat
      421 gatacgcatc aaatattctt gacaatttcc cagtcgtact ccgactggct tctattattt
      481 taccaggttg atttgtttca ccgttagggt catgttcttt ggtcagatgt tcaattaagt
      541 gctctttaaa atactcaata taagaatctt ttttttcccg atgatgagaa ctatgaagtt
      601 ctttccactt ttgttttgtc ataataattt ctttccgagt tatactatct aaaccttata
      661 ctaattatta tatacaactg attaaattat gatcgcaagt ggaaaaaaaa tatgcctttt
      721 ggttttattt tggcttttaa ttgcataacg tatgaacatc acacgcgctt tgtagatcgg
      781 caataaaaca tttgctacaa attaataata gaaccaccca tttgagtctc tatatatttg
      841 ttctgaacta atatctattt tgtttggtta ttaccataga gatcttgagc attgatataa
      901 acccatgcat tcacccctga gttcaatttc acattcacac gtttatattc agagacttca
      961 tatttatcag cttgtgctaa ttcttctttg ctgatagtaa atacgatacc tggaactttg
     1021 tctgagggat tgcccgtata agaaataatg ggatgaaaat tatcgccact tgtcgcaaca
     1081 acggctgagt ttttaatttc aatcgtggtt aatttgaatc cttgcaatac atcttcttgg
     1141 ccttgcattt ttctaccaaa attagcaatt tgtacttttt catagcgtaa ggtcccgtat
     1201 gaaaataact tttctgtgta cgttttgctc ataaaatcaa ccctcataaa ataaaagtag
     1261 tgaattttaa tcattttctt attattatca aagtgagatt tataaaacca ataggagcaa
     1321 atgatgtcaa agttttttaa tttaaagaca ggtaccttac attctgtaga atcctatgcc
     1381 ggcgcagtca aattgcctaa agacataagt caggaatcaa taagaataaa tgagcggatt
     1441 ctggataatg acggctaccc tttgatggca cctaagttaa gacgtcaaaa tgcaaatcag
     1501 ttaagaatgt ttttttccag agaaagtaat actcttttaa atggatcata tgatcttgct
     1561 gatcgttcaa acagggatct gacggaagtt gccaatataa tctcttatag ctaaatataa
     1621 tcaggtattt gccagaataa taaccaggga aatacctgaa aaaccagttt tccactgaat
     1681 gtgtttggtt gcttgctgat tattcttaaa gtcccataga taaacaacaa gctttgaatg
     1741 attcatcctg atgattttat ggttagttgt atggctccaa atcgggtgaa ttgtttatgt
     1801 gctgcaatgt aaagctaaga ttacttatct agtgtctatg tggggttaat actgtacctc
     1861 ctccaataat aggcatagct gttaaatcat ccaacctgat ttatgccatt attaaaaact
     1921 atctaacaaa
//
LOCUS       DAXLDT010000303         1894 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXLDT010000303 DAXLDT010000000
VERSION     DAXLDT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.304"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..311
                     /locus_tag="ACF2SP_003147"
     CDS             <1..311
                     /locus_tag="ACF2SP_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360894.1"
                     /translation="ILNDKENIPLPSSRIKKFTETLQRNDKTLAEHRDPEWKRYVKNC
                     LIAIGVICTGIIPGIVALMAYSTLKGKSSPMFFTNSAGKEYTDKVEKSLTQLPSGPRK
                     "
     gene            complement(452..527)
                     /locus_tag="ACF2SP_003148"
     tRNA            complement(452..527)
                     /locus_tag="ACF2SP_003148"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Glu,seq:ttc)
     gene            complement(584..659)
                     /locus_tag="ACF2SP_003149"
     tRNA            complement(584..659)
                     /locus_tag="ACF2SP_003149"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(623..625),aa:Glu,seq:ttc)
     gene            complement(698..773)
                     /locus_tag="ACF2SP_003150"
     tRNA            complement(698..773)
                     /locus_tag="ACF2SP_003150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(738..740),aa:Ala,seq:ggc)
     gene            1190..>1894
                     /locus_tag="ACF2SP_003151"
     CDS             1190..>1894
                     /locus_tag="ACF2SP_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HFD2360895.1"
                     /translation="MRRILIYIIFFSYFKLGFTHPQMEFQSNMQEINQYIEKTWSILT
                     RDNTNLLADSDEKLPHTQSIIYLPPEENLNKIKNKVTQNWPEWKKKKVIFKYLPRDIS
                     TIKQHGLLYLPHPYIVPGGRFNEMYGWDSYFIELGLIEHNRLTMARNMIDNLIYEIGH
                     YGTILNANRTYYLQRSQPPLLTAMILAYYEKTQDKKWLASTLPAIKKLHTYWTTPPHL
                     IPDLGLSRYYAGGQGKP"
BASE COUNT          655 a          384 c          335 g          520 t
ORIGIN      
        1 acattttaaa tgacaaagaa aacataccat tacctagttc acgaataaag aaatttactg
       61 aaaccctcca aagaaacgat aaaaccttag ctgagcatcg tgatccggag tggaaacgtt
      121 atgtaaaaaa ttgtctcata gctattgggg ttatttgcac tggaatcata cctggaatcg
      181 ttgcgttgat ggcttatagc acgcttaaag gaaaatcgtc tccaatgttc tttactaata
      241 gtgctggcaa ggaatacacg gacaaagtag agaaatcgct tacacaatta ccgtctggcc
      301 ctcggaagta acagttaaga agaatttggg caacttacag ttatattata tgtggctgac
      361 taaagtactc ctaaaattga gatgagccag tataatttat ccaaggtaag gaaagacttg
      421 aagcatataa ttttttaggt aaacaattat atggcgtccc cagggggatt cgaacccccg
      481 ttaccgccgt gaaagggcga tgtcctaggc ctctagacga tggggacctg gatcttatta
      541 agattacatc aaaacactac tacactattt aaatgccaaa taatggcgtc cccaggggga
      601 ttcgaacccc cgttaccgcc gtgaaagggc gatgtcctag gcctctagac gatggggacc
      661 tagtactttt tttaccatca acatacaacg atgttgatgg tggagctagg cgggatcgaa
      721 ccgccgacct cttgcatgcc atgcaagcgc tctcccagct gagctataac cccaaaataa
      781 ggtgtgcagt ttaattagac gcattaatac tgtcaagtgg tttttctatt ttaatgcaag
      841 tcttttataa gacaatcttt ttttacatca aaatcataaa taaatccaaa tcacagcgct
      901 aattttatca atagaaatga acatgagtaa aagcatcatt gcagcaaaaa tttatgactg
      961 taagagtcta atccaaaaaa tatttgacca gcacgaaaac aaaggttgta atagatcttt
     1021 acataacctg attattttgt tttaaggctg agcaccagaa aaggtttgta actcagcaat
     1081 aaaatgaaag agacaggtta agcaaaaagt ctaataaaca tcagacttta aattctgttt
     1141 aaaccaggaa agttaaacat tttttaatca caaaattaca gggaaacgga tgcggcgtat
     1201 tttaatctat attattttct tctcttactt taagcttggc tttacccatc cccagatgga
     1261 gtttcaatcg aacatgcagg aaattaatca atacatagaa aagacctgga gtatactaac
     1321 cagagataat accaatctct tggctgactc agatgaaaaa ctacctcaca ctcaatcaat
     1381 catttactta ccgccagaag aaaatctcaa taaaataaaa aacaaggtca cccaaaattg
     1441 gcctgagtgg aaaaaaaaga aagtcatttt taaatacctt ccccgagaca tttcaacaat
     1501 aaaacagcat ggtttactgt atttacctca tccctatatt gtacccggag gaagatttaa
     1561 tgaaatgtat ggctgggata gttattttat agagttgggt ctaatagaac ataatcgtct
     1621 gacaatggct cgaaacatga ttgataatct catctatgaa attggccatt atggaactat
     1681 tctaaacgct aacagaacct actacctcca acgttcacaa ccccctttac tcacagcaat
     1741 gattttagct tattatgaaa aaacccaaga taaaaaatgg cttgcatcga ctttaccagc
     1801 catcaaaaaa cttcatactt actggacaac tcccccccac ctaatccctg atctcggttt
     1861 atctcgttat tatgctggtg gccaaggaaa gccc
//
LOCUS       DAXLDT010000304         1867 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXLDT010000304 DAXLDT010000000
VERSION     DAXLDT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.305"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            303..>1867
                     /gene="rtxA"
                     /locus_tag="ACF2SP_003152"
     CDS             303..>1867
                     /gene="rtxA"
                     /locus_tag="ACF2SP_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry virulence factor RtxA"
                     /protein_id="HFD2360896.1"
                     /translation="MLAESVIGIVRAVNGLLEKVNAQGQASLVKSGARLQEGDVLTLL
                     SGEAYIQFIHGFPEALALGKPVNLYGVSPALQYGVEELNEQLVQEALAKGIDPSVILD
                     VLGSAAAGAEAVGSGGDAFIMDPLFGFGHVTAGYPTGPISFAYEADTQQLFWFVPEET
                     GVIAESELTQEPESIPQIPQFTTNQAVLTVFEDALPSGIADSAGQARIASSSLSSLLT
                     SSADVAASFAFNSNLSALPTLKSGGIDLSYELSADKRTLTLRESNTQGPGAEVMKFEL
                     TADGQLTQTLMNSIDHPTADSDDGEWMRLDLSPLIDVTFTRTIDGSVLESRTLPANAV
                     VAGIQDDVPIARAQLTNNEILLDETIGMKVGDVDAANDDFNPTTTADPFNNTYGIPIG
                     LVQNANLLDTSTSEMGGDYKNATMTHLIKITDAVSGLQTTDGTPVNLFLESNGDISGR
                     AGDIGAPAVFAIRMNPNTGAITVAQYGSIKQFDTNSYDEAVDLTGRISVVVTAKDSDG
                     DVSNAEIPIGQLI"
BASE COUNT          583 a          359 c          387 g          538 t
ORIGIN      
        1 aaaaattctt tatcaaatca tctaatttat caataatata atattccttt acaattttgt
       61 gatcatgaaa tcattctaat agccaaatga aagattatct gaagctcttt tcatttactc
      121 tatccaatcc aggtaagtca atggcatata tcaaaactgt taaagaaaaa aacctgcaaa
      181 aacaagacat aattatatca atatgattca aatgtttaca tttttgttac atttataccc
      241 cgcaccatca taatccctga actattatta atatgaataa tagtatattt tgaggacaga
      301 acatgttagc tgaatctgtt atcggtatag taagagccgt caatggactg cttgaaaaag
      361 taaacgcaca aggacaagca agcctggtaa agtcaggtgc tcgactacag gaaggggatg
      421 tacttactct attaagtggt gaagcctata tacagtttat ccatggattt cctgaagcac
      481 tggctttggg aaaacctgtc aatttgtatg gtgtttcacc ggcattgcag tacggcgtgg
      541 aagaactcaa tgaacaactg gtccaggaag cgctagccaa aggtattgat ccttcagtca
      601 tacttgatgt tcttggctca gcagcagcag gtgccgaggc ggtgggatca ggaggcgatg
      661 catttattat ggatcctttg tttggcttcg gacatgtcac agcaggatat cccaccggac
      721 ctatttcctt tgcttatgag gcagacactc agcaattatt ttggtttgtg ccagaagaga
      781 caggggtgat tgcagaatcc gaattgactc aagagcctga atctatcccg caaattcctc
      841 aattcaccac aaatcaagca gtgctaacag tttttgagga tgctttgcca agtggcattg
      901 ctgattcagc aggacaagca agaatagcaa gttcttcttt aagttctttg ctcacctctt
      961 cagcagacgt tgctgcttcc tttgctttta atagcaattt aagtgctctt cctacattaa
     1021 aatcaggtgg tatagacctt agttatgaat tatccgctga taaacgtacc ttaaccttaa
     1081 gagaatcaaa cactcaaggg ccaggggcag aggtcatgaa atttgagctg acagcagatg
     1141 ggcagttaac tcaaacacta atgaattcaa ttgatcaccc aacagctgat agcgatgacg
     1201 gcgagtggat gagattagac ctttcacctt taattgatgt gactttcaca agaacaattg
     1261 atggcagtgt tttagagtca agaacactac ctgctaatgc agttgttgca ggaattcaag
     1321 acgatgttcc aatagcaagg gctcaattga ccaataatga aatcctgctg gatgaaacta
     1381 taggaatgaa agtcggtgat gttgatgcag ctaatgatga ttttaatcca acgaccactg
     1441 cggatccttt taataatact tatggtatac ccataggtct agtgcaaaat gcgaatctcc
     1501 ttgataccag tacttctgaa atgggggggg attataaaaa tgcaacaatg acccatttga
     1561 taaaaattac cgatgctgtt tcaggattac agacaactga cggcactcct gtcaatctct
     1621 ttttggaatc caatggtgat attagcggaa gagcaggtga cattggtgct cctgcagttt
     1681 ttgcaattcg catgaatcca aatactggtg caattaccgt tgcacagtat ggttcaatta
     1741 aacaatttga taccaacagc tatgatgaag cggttgactt gactgggcgc atttcagttg
     1801 tagttacagc caaagacagt gatggtgacg tatctaacgc agaaataccg ataggacaat
     1861 tgatcat
//
LOCUS       DAXLDT010000305         1846 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXLDT010000305 DAXLDT010000000
VERSION     DAXLDT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.306"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(79..1131)
                     /locus_tag="ACF2SP_003153"
     CDS             complement(79..1131)
                     /locus_tag="ACF2SP_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HFD2360897.1"
                     /translation="MHHLKLAERLGIQFPIIQAPMAGGATTPELVAAVSNSGGLGSLG
                     AGYMRPDEIRQAIIKIRQLTSKPFAVNLFIPEAHHATPEQIQSACDDINLCCTELNIE
                     ISPVSKPYSLPFVDQMQILIEEKIPVFSYAFGTLEPMWIKQLKKNGTFLIGTATTIHE
                     ARILEASGIDAIVAQGSEAGGHRGTFIGNAEEALIQLSELIPQLVEAIRVPVIAAGGI
                     MNGKGIISAINSSASGVQMGTAFLSCFEAGISYKYKQTLLSQQQDNTVLTRAFSGKLA
                     RGIRNKFITCMDNRKINILDYPIQNALTQVMRQKARENDNIDFMSLWAGQSAHLCRSM
                     SASDLISTLVIEAEMK"
     gene            complement(1109..1720)
                     /locus_tag="ACF2SP_003154"
     CDS             complement(1109..1720)
                     /locus_tag="ACF2SP_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD2360898.1"
                     /translation="MKKIAPTLKNEIINTAIDIAQDTSWEKVRLIDIAKALNIDLNTI
                     RYFFAEKEQIIDGCFDRADEAMLNASSRPKFDLLTAKEKLHDLLMTWLSTFEGQQKVI
                     RQMIFHKLEPGHLHLQLPAIKRISQTVQWWREAAGLRETFIQRAIQETGLTIIYLATF
                     SYWLLDNSTERLATRLFLENKLNMAKNLSNFWGFTYYAPSKVS"
BASE COUNT          516 a          361 c          345 g          624 t
ORIGIN      
        1 actccctcat taaaaacatt ttttgcctcg actttgaggt ttttgtaagt cctgtttttg
       61 taatcattca cctctctttt acttcatttc tgcctcaata actaaagtac ttattaagtc
      121 acttgccgac atagatcggc ataaatgagc cgattgtcca gcccataacg acataaaatc
      181 aatattatcg ttttctctgg ctttttgtct cattacttgg gttaatgcat tttgaatggg
      241 gtagtcaagg atatttattt tccgattatc catgcaagta atgaatttat ttctaattcc
      301 acgagcaagc tttccagaaa aagcacgtgt tagcacagta ttatcttgtt gttgtgataa
      361 tagggtttgt ttgtatttat aagagatacc agcttcaaag caacttaaaa aagcggttcc
      421 catttgtacg ccagaggcac ttgaatttat ggcagagata atgcctttac cattcatgat
      481 tcccccagcg gctatcactg gcactcttat tgcctcaact agttgaggta tcagttcaga
      541 tagttgaatc aaagcttcct cagcattgcc gataaaagta cctcgatgcc ctcctgcttc
      601 acttccttgt gcaacaatgg catcaatccc acttgcttca agtattcttg cttcgtgtat
      661 agtggttgcc gttcctatca gaaaggttcc atttttcttc aattgtttga tccacattgg
      721 ttctaatgta ccgaatgcat agctaaagac agggattttt tcctctatca ggatttgcat
      781 ttggtcaaca aaaggtaggg agtagggttt actcacggga ctgatttcaa tatttagttc
      841 agtgcagcac aaattgatat catcacaagc gctttgaatt tgttccggtg ttgcatgatg
      901 tgcttcgggt ataaaaagat taacagcaaa aggtttgctt gttaattggc gaatttttat
      961 aatagcttgt ctgatctcat caggtctcat ataccctgcg cccagagagc ccaaaccacc
     1021 actgttagaa accgctgcaa ccaattccgg agtagtagct cctccggcca taggtgcttg
     1081 aatgatagga aattgaatac caagcctctc agctaacttt agatggtgca tagtaagtaa
     1141 aaccccaaaa attggataaa ttttttgcca tatttaattt gttttccaaa aaaagacggg
     1201 ttgcgagacg ctctgtggaa ttatctaata accaataact aaaggtggcc agataaatga
     1261 tggtcaatcc ggtttcctgg atggcacgtt gaataaaggt ctctctaaga ccagctgctt
     1321 cacgccacca ttgaacagtt tggctgatgc gtttgattgc aggcaattga agatgaagat
     1381 gaccgggctc taatttatga aatatcattt gccggattac tttttgctgg ccttcgaaag
     1441 tacttaacca agtcattaat aaatcatgca gtttttcttt ggcagtgagt aaatcaaatt
     1501 tcggtctgga ggacgcattg agcatggctt catcagcacg atcaaaacag ccatcaatga
     1561 tttgctcttt ttccgcaaaa aaatatcgga tcgtatttaa atcgatattt aatgctttag
     1621 cgatatcaat cagcctcact ttttcccaag aggtatcctg ggcaatgtct attgctgtat
     1681 tgatgatctc attttttagt gttggtgcta tttttttcat gaaacacctc cctgtctctt
     1741 attagtttat tactaatttt atgatcagga ttaatttttg atggttttgg tagttaaatt
     1801 aactataagt taaatcaaaa aatgctattg gttatttcaa ttagtc
//
LOCUS       DAXLDT010000306         1832 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXLDT010000306 DAXLDT010000000
VERSION     DAXLDT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.307"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            147..995
                     /locus_tag="ACF2SP_003155"
     CDS             147..995
                     /locus_tag="ACF2SP_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD2360899.1"
                     /translation="MCKEINIIWVGPQRLPYKYLQHLKKWHNLNPDHQINFITDEHSS
                     SIQLPGINTIHWKQCFDLTDVRQEFLLREAERALREKAYATLSNIVRKVKIFNDGGYY
                     FDTDIVPIEKLPAITENTPFLSQPKASYGAEFAKHRFVVSALYGSKHSPVFNEALTIM
                     HETYQVKSLQDAIKENPEKSDYLRSYYSASLMLSRSVKRLYTNEQDYQKMIEDCQNEF
                     GSPTAFQIIQDGSWGEGLNAVLMESKHLAATAIQRFFRAAQALNQVNSNQSTSHMQSP
                     EKPRFF"
     gene            complement(1132..1560)
                     /locus_tag="ACF2SP_003156"
     CDS             complement(1132..1560)
                     /locus_tag="ACF2SP_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360900.1"
                     /translation="MYLSRCISHGAIAGIIAGIVFTFFLVMGGMLETLGSMINMPTKG
                     GGLLVHAVMSIGSGIAFALVLDWLMHSWISAILLGLLFGFAMWVGGPMTVLPYFASGV
                     PLFAKWTMEGFKQNIPPLVGHLVYGLILGVTYYAFKRRNK"
BASE COUNT          616 a          388 c          292 g          536 t
ORIGIN      
        1 caagcctaaa cctataggta tcctattgga aaatcaatcc ttcatctttc agacaaatga
       61 aatcccccac ctcaattgtt tattttattg acattctgaa tacattgcta taaaaacagt
      121 tttttctaat tagcggtaaa cacaatatgt gtaaagaaat taatatcatt tgggtaggac
      181 cacaacgcct tccctacaaa tatctacagc atttaaaaaa atggcataat ttaaacccag
      241 accatcaaat caatttcatc actgatgaac attcttcttc tattcagctt cccggaataa
      301 ataccattca ctggaaacaa tgttttgacc tgacggatgt acgacaagaa tttttgctga
      361 gagaagcaga acgtgctctc agggaaaaag cctatgctac tttatcgaat attgttcgca
      421 aggttaaaat ttttaatgat ggcgggtatt attttgatac tgacatcgtt cctatagaga
      481 aattgcctgc tattactgaa aatacccctt tcttgtcaca acctaaagca agttatggtg
      541 cagaatttgc caagcatcgg tttgttgttt ctgcgcttta tggtagcaag cactctccag
      601 tattcaatga agcattgacc atcatgcatg aaacttatca agtaaaatca ttgcaagatg
      661 ccattaaaga aaatccggag aaatcagact accttcgctc ctattattca gcctctctta
      721 tgttatcccg ttctgttaaa agactttata caaatgaaca ggattatcaa aaaatgatag
      781 aagattgcca aaatgagttt gggagcccta ctgcatttca aattatacaa gatggttcat
      841 ggggagaggg attaaatgct gttttgatgg aaagcaaaca cctggcagca actgccatac
      901 aaagattttt tcgagctgcg caagctctga atcaagtgaa ttccaatcag tcaacgagtc
      961 acatgcaatc ccctgaaaaa ccaagatttt tttgaagagg ccatgggctt gattgcgagg
     1021 tatgcaattg tttttgctaa attgaggtgg cgattcctat aaaaaacaat caccaccttt
     1081 ttatgtcatt atatctttaa taaaatcaat tttttacgct gaaaaatgat gtcacttatt
     1141 cctgcgcttg aaagcataat aagtaacacc caatatcaac ccatacacaa gatgcccaac
     1201 caaagggggt atgttttgtt taaatccttc catagtccat tttgcaaaca aaggcacccc
     1261 tgaagcgaaa tagggtaaca cggtcattgg gccgccgacc cacattgcaa aaccgaacaa
     1321 taacccaaga agaatggctg agatccagga atgcattaac caatccagaa ccagggcaaa
     1381 agcgatcccg ctgccaatgc tcattacagc atgcaccaaa aggcctcctc cttttgttgg
     1441 catattaatc atacttccca atgtttcgag catgcccccc ataactaaaa aaaaggtaaa
     1501 aacaattcct gctataattc ctgcaatagc gccatgcgaa atacatcttg aaagatacat
     1561 gccaatctcc ttattaaata gatcccagtt taccattcaa acaggactta cgaaaaaccc
     1621 caatctctat gttaaaaaat gtttttaatt cggtcattta gtttatctaa actccctcat
     1681 taaaaacatt ttttgccttg accttggcgt ttttcgtaag tcccgtcaaa atcatcagtt
     1741 tacttaataa tcaacaatta aggtagaaaa attaatggca atgtcttaaa aaattttttg
     1801 ccagccacat catcaacatc ttattgttaa ta
//
LOCUS       DAXLDT010000307         1820 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXLDT010000307 DAXLDT010000000
VERSION     DAXLDT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.308"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..426)
                     /locus_tag="ACF2SP_003157"
     CDS             complement(<1..426)
                     /locus_tag="ACF2SP_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360901.1"
                     /translation="MKKCVLIVDPFSSGALYAEALIKRGIVPVAILSHYPIIEVYRDS
                     FKSSDFKYIFTYSENADFYEELNIFLRDKELIVVLAGAESGVELADKLSERYCIDHSN
                     GTALSSCRRNKYEMVQKLGQLHIDVPLTIKSNSSDEFLTW"
     gene            complement(461..1669)
                     /locus_tag="ACF2SP_003158"
     CDS             complement(461..1669)
                     /locus_tag="ACF2SP_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360902.1"
                     /translation="MVRHLKLIIILAFTLAGDILFGLALIWRVLALDGSAKTLGLFLC
                     VITLVTFALQKSSVGFRRMLEKTPRRSFMWIRGVGVGFSIIFIPLLWHETTWLLYLVG
                     AIATIINFLSILTLEAMMAHEVLEKRISSNKASRILQIAILTSSFLGASLLGTILALG
                     NIVAVLLFCIATYLVGAICFSTQSDVGIQKISQVQTAKTASNQKMTYGNNLSVLRLAF
                     IGLTLLMTQISAFNFLLPLIAQHEKFWTASQYGFIDAAAELGGFLATIIMFEKGIFSK
                     LWIFAFLGIALVDILFFAVTDIYLISTATLFLGFCVSCFRIKQREYIYDSVSNTEEIL
                     EWTGRITAMTAIIKALTPLGLAFFISQPSFDLMIIGIVVGTVMLIVTSVLYFYTNKEF
                     KQQIPSYSAV"
BASE COUNT          641 a          338 c          282 g          559 t
ORIGIN      
        1 ccacgtaaga aactcatcag atgaattact tttaattgtt aaaggaacat caatgtgaag
       61 ttggcctaat ttctgtacca tttcatattt atttcttcta caactgctta aagctgtccc
      121 atttgaatgg tcaatgcaat atctttcaga cagcttatct gctaattcaa ctccagactc
      181 agctccagct aaaactacta ttagctcttt atctcttaaa aatatattta attcttcata
      241 gaagtcagca ttttcactgt aagtaaaaat atatttaaaa tcagaagatt tgaacgaatc
      301 tctataaact tcaattattg gataatgtga taagatggca acaggaacaa tgcctcgttt
      361 tatgagtgct tcagcgtata gtgcacctga agaaaagggg tcaacaatta aaacacattt
      421 tttcatatca atctcacttt atataaattt aatttattaa ctaaaccgca ctatagcttg
      481 gtatttgttg tttgaactct ttattcgtat agaaatacaa tacggatgta actattaaca
      541 ttaccgtacc caccacaatt ccaattatca ttaaatcaaa cgagggttga gaaataaaaa
      601 aagctaagcc aagcggcgta agagctttta taatggctgt cattgcagtg atgcgtccgg
      661 tccattcgag tatttcctca gtatttgata ctgagtcgta gatatactct cgttgtttta
      721 tccgaaaaca actgacacaa aatccaagaa ataatgtagc tgtgctaata agataaatat
      781 ctgtcactgc aaaaaataaa atatcgacta atgcaattcc gagaaatgcg aatatccata
      841 gctttgaaaa aattcctttt tcaaacataa taatcgtagc taagaaccca cctaactctg
      901 ctgcagcatc aataaatcca tattgagagg ccgtccaaaa tttctcatgc tgagcaatta
      961 aaggtaacaa gaaattaaag gcagagattt gtgtcattaa caatgttaga ccgataaatg
     1021 ccagccttaa aactgataag ttatttccat aagtcatttt ctgattagaa gctgtttttg
     1081 cagtctggac ttgactaatt ttttggatgc cgacatctga ctgtgttgaa aaacatattg
     1141 cgccaactaa ataagtcgca atacaaaaaa gtaaaacagc tacaatatta ccaagtgcta
     1201 atattgttcc aagcaaactg gcacccaaaa aagatgaggt caggatagct atttgtagaa
     1261 tacgcgaggc tttattcgag cttattcttt tttcgagcac ttcatgagcc atcattgctt
     1321 caagggttaa gatgctcaaa aaattaataa ttgttgctat cgcacctact agataaagaa
     1381 gccacgtagt ttcatgccat aatagcggta tgaatataat agagaaccca acgcctactc
     1441 ctcttatcca cataaaagag cgacgtggtg ttttttctag catccttctg aagccaacgc
     1501 tagatttttg taatgcaaaa gttacaagag taattacaca aagaaaaagg cctaatgttt
     1561 ttgctgaacc atctaaagct aaaactctcc agatgagcgc cagcccaaat aatatgtcgc
     1621 ctgcaagagt aaatgctaat atgattataa gttttaaatg acgtaccata ataataatcc
     1681 ttataataat ccttttaata tttaaatata aaaaatctga tccttaacac gaacagccgg
     1741 gattgcattt tttattaatg aagaaatctt aaaataccca tcatgccaag gacattgaat
     1801 cctttgtgat tctcggcata
//
LOCUS       DAXLDT010000308         1797 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXLDT010000308 DAXLDT010000000
VERSION     DAXLDT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.309"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..696
                     /gene="ceg18"
                     /locus_tag="ACF2SP_003159"
     CDS             <1..696
                     /gene="ceg18"
                     /locus_tag="ACF2SP_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg18"
                     /protein_id="HFD2360903.1"
                     /translation="LSAVSRVINNFSTEIKNRVVEVGSNLKKSTEEFIGEVVIKAAVN
                     RLKQGMLNALSSSNDTTKDNGNDLSTNSPDIEDVSAPTLSSKKKTEANTTLVGSTGIM
                     QKLLGNIGVNSEQSIEVLSKHFNGAVERFTQDVSKAANEAVCEKIKSIQPEDVVKGIN
                     SLIGNTLKVDSESGDTTLSFKLESAVQILGVILAAMGMLMFASQTKSTEQKTLEIEHL
                     PTEEIQPPSLAIH"
     gene            969..>1797
                     /locus_tag="ACF2SP_003160"
     CDS             969..>1797
                     /locus_tag="ACF2SP_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFD2360904.1"
                     /translation="MNPILNLIISFIALLWAANHLVAGSSGLAIRFQLSPLMIGLTIV
                     ALGTSAPELFISIISSLKDKNDLAIGNAIGSNIANIGLILGIIILIKPTSFHFNKLKK
                     VYPILIIVMLFAYSLILDGYLGTIDGCLFLIGCIAVLCYFIYLASHSPKRDLSVNEFR
                     PAILSYRSTPSNLLSIAIGLLILPICSKYLVYNASEIAKWAGISEFTIGLTIIAIGSS
                     LPELTTALVAAIKGEDSIAIGTIIGSNIYNLLLILALPGLLNPTKISSVVLWRDMPVM
                     "
BASE COUNT          556 a          334 c          332 g          575 t
ORIGIN      
        1 ttatctgctg tttcgcgtgt cataaataat ttttcaactg aaattaaaaa tagagttgtt
       61 gaagtcggga gtaatttaaa gaagtcaaca gaagagttta ttggcgaagt agtcataaaa
      121 gcggctgtga atcgtttgaa acaaggaatg ttgaatgctt tgtcttcttc gaatgacacc
      181 accaaagata atggtaatga tttatccaca aacagccctg acattgaaga tgtatctgcc
      241 cccacgctaa gctcaaagaa aaaaacagag gcaaatacca ctctggttgg cagtaccggt
      301 ataatgcaga aattgttggg caatattggc gtaaattctg aacaaagtat agaagtgttg
      361 tccaagcatt ttaatggtgc tgttgagcgt tttacccaag atgtttcaaa ggctgcaaat
      421 gaggctgtgt gtgaaaagat caaaagtata cagccggaag atgtggtaaa gggtattaac
      481 agtctaatcg gcaatactct caaagtagac tcagagtctg gcgacactac cctatcattc
      541 aagcttgaga gtgccgtaca aatcctgggc gtcattcttg cagccatggg tatgctgatg
      601 tttgcttctc aaactaaatc aaccgagcag aaaactctag agatagaaca tctgccaact
      661 gaagaaattc aaccaccttc attggctatt cattaagaaa tactcacggc ttgttcttcc
      721 aggctacgta ccgcaaatca ttcttggtat gtagcttgaa gaattggcaa cacattcata
      781 aactccttaa ttacaagatt tgattacaga acttgccgtg cttcatgata tacaagcatt
      841 ttttaattaa catgatgatt acatgagctc ctggcaaaac ctgaatataa agtgacactt
      901 aatatctata agttatatcc tataaaccat tctcgtatta ttatcactaa aaaatatcga
      961 gtacaaaaat gaaccctatt ctgaatttga ttatcagttt tattgccctt ctttgggctg
     1021 ccaatcattt ggttgctggt tcttcagggc tggcaattcg ttttcaatta tcacccctca
     1081 tgattggttt aactattgtt gcattgggta ccagtgctcc cgaattattt atctctatca
     1141 tttcttcctt aaaagataaa aatgatctgg caattggaaa tgcgattggt tcaaatatag
     1201 caaatattgg cttaatttta gggattataa tccttatcaa accaacctct tttcatttca
     1261 ataaactaaa aaaagtatac ccaatattga taattgtcat gctgtttgcg tacagcttaa
     1321 tacttgacgg ttatttgggt acaattgatg gttgcctttt tttgatcgga tgtatagcag
     1381 tactctgtta tttcatttat ttagctagtc actcgcctaa aagagattta tcagttaatg
     1441 aatttagacc cgcgatacta tcctatcgtt caacaccctc taatttattg agtatcgcaa
     1501 taggtttatt gattctgcct atttgttcaa aatacctggt ttataatgca tctgaaattg
     1561 caaaatgggc tggtataagt gaattcacca ttggcttgac cattattgct ataggttcct
     1621 ccttgccaga acttacaacg gctcttgttg ccgctatcaa aggagaagac tctatagcga
     1681 taggtacgat aattggctca aatatttata acctgttgtt aatcttagct ctaccgggat
     1741 tactaaaccc aactaaaatt agcagcgtcg tattatggcg tgatatgcca gtgatgc
//
LOCUS       DAXLDT010000309         1725 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXLDT010000309 DAXLDT010000000
VERSION     DAXLDT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.310"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1496
                     /locus_tag="ACF2SP_003161"
     CDS             <1..1496
                     /locus_tag="ACF2SP_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD2360905.1"
                     /translation="KVEINEYRQIANKFIDEHRQIINLQKEKAYYEVRKNIAELVAHD
                     IRSPLAAINTAVADVTSIPENKRIMIRNAAKRINDIANNLLIQSKNNLLDCKSSEINM
                     NNSPELIFVVLDNIVAEKRYEYYGTNIQINLLGSDYSYNCFSNIHPDSFKRVLSNLIN
                     NGIEAVNSNGSIIISLTCNATHVEIIIEDNGCGIPRDILPKVTEQGFSFNKKTGAGFG
                     LSYAKQYLEQINGSMHIHSEEKVGTKIQITLIRSTPPSWFCDSMNIRHGTNIVVLDDD
                     ISIHDAWNERFSNISHVKMFHFYNAIDLLQYKVDPEIPVLYLVDYELLADVKNGLDVI
                     EELNLNGKAILVTSCFEDIAIRNRCENIGVKIIPKSYVPYIKIIETPKKEHINTVVFI
                     DDDEMMRMTWIFAAEEAGQCISTYSSFDEFINEINNYGENTLIYIDSDLGNNVRGEDC
                     AKLLFDKGFIDVHLATGHSRDRFSQMPWIKSIVGKEPPFRFAHGNLT"
     gene            1493..1720
                     /locus_tag="ACF2SP_003162"
     CDS             1493..1720
                     /locus_tag="ACF2SP_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360906.1"
                     /translation="MNSTDKDKLKHDFLNSIVIINSMTKSASSFINTISQSMETSDIN
                     QKQMEKFLYSMSTIREQTVKIENYFLSLLNE"
BASE COUNT          610 a          270 c          289 g          556 t
ORIGIN      
        1 caaaagttga aattaatgaa tacagacaaa ttgctaataa atttattgat gaacatcgac
       61 aaataatcaa tttacagaaa gaaaaagcct attacgaggt aagaaaaaat attgctgagc
      121 ttgttgctca cgatattcga tcaccattag cggcaataaa taccgcagta gctgatgtaa
      181 catcaattcc tgaaaataaa cgcattatga tcagaaatgc agctaaaaga atcaatgata
      241 ttgctaataa cctactcata caatcaaaaa ataatttgct tgactgtaag agtagcgaaa
      301 taaatatgaa taattctcca gaattaatat ttgttgtttt agataatatc gttgcagaaa
      361 aaagatatga atattacgga accaacattc aaatcaactt acttgggtcg gactactcat
      421 ataattgttt ttctaacata caccctgatt cttttaagcg tgttctatcc aatctcatta
      481 ataatggtat tgaagcagtt aattctaatg gctctattat tatctctcta acctgcaatg
      541 ctacccatgt cgaaattatt atagaagata atggctgcgg cattcctcgc gatatcttgc
      601 caaaggttac ggagcaagga tttagcttta ataagaaaac tggtgctggt tttggcttat
      661 cttatgctaa acaatattta gagcaaatca atggttcaat gcatattcac tccgaagaaa
      721 aagttggaac aaaaattcag atcactctaa ttcggtctac ccctcctagc tggttctgtg
      781 attcaatgaa tattaggcat ggtactaata ttgtcgtatt agatgacgat atatccatcc
      841 atgatgcatg gaatgaacgg ttttcaaata tttcacacgt taaaatgttt catttttata
      901 acgcgatcga cttattacag tataaagttg atccagaaat cccagtatta tatttagtag
      961 attatgagct gctcgctgat gttaaaaatg gtttagatgt gattgaagag cttaatttaa
     1021 atggcaaagc tattttagtt accagttgct ttgaagatat tgctatacga aatcggtgcg
     1081 aaaatattgg tgtaaaaatc ataccaaaat cttatgtacc ctatattaaa attattgaaa
     1141 ctccaaagaa agaacatata aatacggttg tttttattga tgacgatgaa atgatgcgaa
     1201 tgacttggat ctttgctgct gaagaggctg gacaatgtat ttcaacttat tcttcatttg
     1261 atgagttcat aaatgaaata aataattatg gtgagaatac tcttatttat attgattcag
     1321 atctaggaaa caatgttaga ggcgaggatt gtgcaaaact cctttttgat aagggcttta
     1381 ttgatgttca tcttgccact ggccattcaa gagatcgatt tagccaaatg ccatggatta
     1441 aatccattgt tggtaaagaa ccaccttttc gatttgcaca cgggaattta acatgaactc
     1501 aacggataaa gataaattaa aacacgattt tttaaactca attgttatca tcaacagcat
     1561 gacaaaatcg gcttcgagtt tcattaatac aatttcacag tcaatggaga ccagcgatat
     1621 taatcaaaaa cagatggaaa aatttttata ttccatgagc actattcgtg agcagactgt
     1681 aaagattgaa aattattttc tatctctttt gaatgaataa ttctg
//
LOCUS       DAXLDT010000310         1711 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXLDT010000310 DAXLDT010000000
VERSION     DAXLDT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.311"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(42..1124)
                     /locus_tag="ACF2SP_003163"
     CDS             complement(42..1124)
                     /locus_tag="ACF2SP_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HFD2360907.1"
                     /translation="MDKTPVVKVHSGDSVSLETWNSCLHEMVFNKTTPAEIAKFYTKH
                     DIQKRRGMHSLTGPVYVEGAEPGDVLEIRVLDIKLNDFGYNFLSPTQGILSEFTKPRI
                     MYFKYDKEKNTTEFTKGICLHLKPFQGIIAVAPPLDWPDGWPVNLETQMGQPVPGQFN
                     SVPPGPFGGNLDEPSLQVGSILYLPVFQKGALVWTGDSHAMQSNGEIDVTALETSFEG
                     ITLQFIVRKDLKAEGVFDKTKRNGNSLFTWPIVENSEEWILIGLHENLFEAMQLASKN
                     AIEFLVRTQGLSSQESYQFLSMVGNFDIPEAVNVIKSLAVHIPKEIFKNTGKMTSLSS
                     EGKFPLTTPRTNENTTCVPQEGKIIE"
     gene            complement(1158..>1711)
                     /locus_tag="ACF2SP_003164"
     CDS             complement(1158..>1711)
                     /locus_tag="ACF2SP_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFD2360908.1"
                     /translation="VWSDRGIWSELMSVVSDVDIQTVMIDATIVRAHACASGYVKDGN
                     ETQALGRSKGGFTTKIHALVDGVGNPIKFILTGGHRNDITQAAELLKEQHDTIVLADK
                     GYDSQELIDTLHHQRCSPVIPSRKNSKYPRKIDKQLYKERFLTEAFFSKLKHFRRIFS
                     RFDKTTSAFSGFLHLAGALIWMR"
BASE COUNT          487 a          357 c          331 g          536 t
ORIGIN      
        1 cataaataaa ttaaaatttt attcattatt tcacctaaag gttattcaat aattttgcct
       61 tcttgaggga cacaagttgt attttcatta gtccttggcg ttgttagagg gaacttgcct
      121 tcagagctaa gcgaagtcat cttcccagtg tttttgaaga tttctttagg aatgtgaacg
      181 gctaggcttt taataacatt gactgcttca ggaatatcaa aattacctac catgctcaaa
      241 aattggtaac tttcttgcga agatagtccc tgggtacgta ctaaaaattc aattgcattt
      301 ttagaggcga gctgcatggc ttcaaacaaa ttttcatgta aacctattag aatccactct
      361 tcagaatttt cgacaatggg ccatgtgaat aaactgtttc catttcgctt ggtcttgtca
      421 aatacccctt cagcttttag atcttttctt acaataaatt gcaaggtgat cccttcaaag
      481 gaggtctcta gtgctgtgac atcaatttcg ccgttacttt gcatggcatg tgaatctcct
      541 gtccaaacta acgcaccttt ttgaaaaacg ggtaaataaa ggatagagcc tacttgcaag
      601 cttggttcat ccaggttccc tccaaaagga cctggcggga ctgaattgaa ttgccctgga
      661 acaggttgac ccatctgagt ttctaaattc acgggccatc catccggcca atctagaggt
      721 ggagctactg cgataattcc ctgaaagggc tttaggtgta agcatatgcc cttggtgaat
      781 tctgtggtgt ttttttcttt atcatatttg aaatacatga ttcttggttt ggtaaactct
      841 gataaaatcc cttgtgtagg gcttaaaaaa ttatacccaa aatcatttaa cttgatatct
      901 aatacccgaa tctcaagtac atccccaggc tcggcccctt ctacataaac aggtccagtt
      961 aagctatgca taccacgtct tttttgaatg tcgtgttttg tatagaattt tgcaatctca
     1021 gctggcgttg ttttattaaa gaccatttca tgcagacaac tattccaggt ttccaaggac
     1081 acagaatctc cagagtgaac tttaactaca ggtgttttat ccaatgtaaa aagacctagg
     1141 ttctgtgaac gaaattttta gcgcatccaa atgagcgctc cggccaaatg caaaaaccca
     1201 gaaaaagcgg aagtggtttt atcaaaacga gaaaaaattc tgcggaaatg cttcaattta
     1261 gaaaaaaaag cctcggtcag aaaacgctct ttataaagtt gtttgtctat ttttctgggg
     1321 tatttagagt tttttcttga tggaatgact ggagagcagc gctgatggtg tagcgtatca
     1381 atgagttctt gagagtcata tcccttatca gcaaggacta ttgtgtcgtg ttgttccttg
     1441 agtagctctg ctgcttgagt aatatcattg cggtgtcctc ctgtcagaat aaatttaatc
     1501 ggattgccca ccccatctac caaagcgtga attttagtgg taaaaccacc tttgctacgc
     1561 cccaatgctt gagtctcatt tccatccttg acataacccg aggcgcaagc gtgagcacgt
     1621 acaatagttg catcgatcat caccgtttgg atatcaacat cactgacaac tgacattaac
     1681 tcagaccaga tgccacggtc agaccacact t
//
LOCUS       DAXLDT010000311         1686 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXLDT010000311 DAXLDT010000000
VERSION     DAXLDT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.312"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            86..613
                     /gene="aroK"
                     /locus_tag="ACF2SP_003165"
     CDS             86..613
                     /gene="aroK"
                     /locus_tag="ACF2SP_003165"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFD2360909.1"
                     /translation="MSIVKVRNIFLIGPMGAGKSTIGRALAKELKLEFYDSDEVIEER
                     AGADISWIFDIEGEEGFRRREQKVIDELTQKTNIVLATGGGVVITPENRNALAGRGTV
                     IYLKTSLQQQFERTKRDTKRPLLQTEDLEGRLESLRDEREPFYDELADVSFETDKLTV
                     KAVANNIIKYLYGEV"
     gene            600..>1686
                     /gene="aroB"
                     /locus_tag="ACF2SP_003166"
     CDS             600..>1686
                     /gene="aroB"
                     /locus_tag="ACF2SP_003166"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFD2360910.1"
                     /translation="MAKFELYAEVDVSISGHQYPIIICRNGLIDPDLINRFITSKQVL
                     IVTNRTVAPLYLGHLQSVLPSKQCDVVILEDGEEHKNQRSLFTIYDSLIQNKHHRDTS
                     IIALGGGVIGDMAGFAASTYQRGVRFIQLPTTLLAQVDASVGGKTAINHPAGKNMIGS
                     FYQPQAVIIDLNTLKTLPEREFRAGIAEMIKYALLVGGSFFERIQEVLQQGLTVHSPE
                     LPLLIAECCQVKAKIVEQDERESGLRALLNLGHTFAHALETYTDYKKWLHGEAVAIGL
                     YCAAVLSEKKGLLDKPIVDQVEKMLIHAGLPHKIPNSIDLIQLRELMSLDKKIKNNCL
                     RFVMIKKPGACYIDDSVTEDCLHNTLIN"
BASE COUNT          530 a          265 c          384 g          507 t
ORIGIN      
        1 aaatcaacta agacgtgtat ctaatttgtt gttataatag catggctaat tatgacaatt
       61 cagtagaaga ggtaagtaaa aaaaaatgag catagttaaa gtacgaaata tttttctcat
      121 tggtccgatg ggtgctggta aaagtactat aggacgtgct ttggcaaagg agctcaaact
      181 agaattctat gactcagatg aagtaattga agagcgtgcg ggcgcagata tatcgtggat
      241 ctttgatata gaaggtgagg agggatttag gcgccgagag caaaaggtca ttgatgaatt
      301 gactcaaaaa accaatatag tattggcaac gggtggtgga gtcgtcatta cgcctgaaaa
      361 caggaatgcc ctggcaggcc gtggcacagt gatttatctc aaaacatctt tgcaacagca
      421 atttgaaaga acaaagcgtg ataccaaacg tcctttatta caaacagaag atcttgaggg
      481 acgcctggag tcactcagag atgagcgaga acctttttat gatgaattgg cggatgtcag
      541 ttttgaaaca gataaactga ctgtaaaagc tgttgcaaac aatataataa aatacttata
      601 tggcgaagtt tgaactttat gccgaggttg atgtttcgat ttcagggcat caatatccaa
      661 taattatttg ccgcaatggt ttaattgatc ctgatttaat taatcgtttt atcacttcaa
      721 aacaggtgct tattgttact aatcgaacag tggcaccttt gtatctaggc catttgcaat
      781 ctgttttacc atcaaaacaa tgcgatgttg ttattctgga agacggtgaa gagcataaaa
      841 atcagcgcag tttatttact atttatgact ctttgattca aaacaaacat catagagata
      901 cctccatcat tgctttgggc gggggagtga ttggtgatat ggctggtttt gcagcttcta
      961 cgtatcaacg aggtgtgcga tttattcagc tgcctactac gttactggct caggttgatg
     1021 cttcagttgg tggtaaaaca gcgattaatc atcctgccgg taaaaatatg attggcagtt
     1081 tttaccaacc tcaagcggtc ataattgatt taaatacctt aaaaacatta cctgagcgtg
     1141 agtttcgtgc aggtattgcc gagatgataa aatacgcttt gttggttggt ggctcttttt
     1201 ttgaacgaat tcaagaggtt ctgcaacaag gattaacggt acatagtccg gagctaccac
     1261 tactgatagc agaatgttgc caagttaaag ctaaaattgt agagcaagat gaaagggagt
     1321 ccgggttgcg tgctttgctt aatctggggc atacttttgc tcatgcgctg gaaacgtata
     1381 ccgattataa aaagtggtta catggagaag ccgttgccat aggtttatat tgtgcggctg
     1441 tattatcaga gaagaagggc ttgttggata aaccaattgt agaccaggta gaaaagatgc
     1501 tcatacatgc aggtttgcct cataaaattc caaattccat agatttaatt caattaagag
     1561 agttgatgag tttggataag aaaattaaaa ataattgttt acgttttgta atgattaaaa
     1621 agccaggtgc ttgttatatt gatgattcag ttactgaaga ttgtttgcac aatacactga
     1681 ttaatg
//
LOCUS       DAXLDT010000312         1656 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXLDT010000312 DAXLDT010000000
VERSION     DAXLDT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.313"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            115..1464
                     /locus_tag="ACF2SP_003167"
     CDS             115..1464
                     /locus_tag="ACF2SP_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD2360911.1"
                     /translation="MKHITIIGAGLAGILCGLYLARRGYEVELFESRPDIRNSPTDYG
                     RSINLALSCRGITALKAMNLLSEVNKIMVPMRARAIHEANGEVHYQPFGRYIDEYINA
                     ISRSDLNALLLNKAELCPNIKLHFNMKLHSLDIHNKKIKFENKNGDFVEASYHRLIGA
                     DGAPSHVRDMLKNEGIVSASRDFLSHGYKELSISKKHTKGMAREHLHLWPRDSFMLLG
                     NPNPDDSITGSLFLANEGKDSFAELNNEEKLHLFFKTQFPDAYAAMPNLVQEFFGNPT
                     GHLSTIQCSPWYYKDECLLIGDAAHGIIPFFGQGMNSAFEDCRILDELLDEYQDDWSR
                     VNPLFYEQRKVNTDAIAKMSMDNYHEIHSDIRNPKFILQKQIERELMLRYPEHYVSMH
                     VLVMFTNTPYAKAMAIGELQSGLLEQICFPITDIKELNWQEVEKLLGIYDKKLAKII"
BASE COUNT          538 a          248 c          342 g          528 t
ORIGIN      
        1 caggttgagc ctgagtttta cttttattca agcaatatat tgattgaatt tgtgtatttt
       61 ttaaggagat tcggaataga tgtcgtgttg atatagaatg ggttgggagg ataaatgaag
      121 cacattacca taattggagc cgggttagcg ggaattttgt gtggtttata tcttgctaga
      181 agaggctatg aagtagagtt gtttgaatca agaccagaca ttcgtaatag tccgactgat
      241 tatggacgct ccattaatct tgctttgtca tgcagaggaa ttaccgcttt aaaagcaatg
      301 aacctgttaa gtgaagtaaa taaaattatg gttccaatgc gggcaagagc tattcacgaa
      361 gccaatggcg aggttcacta tcaaccattt ggacgttaca ttgatgaata tatcaatgct
      421 atttctcgtt ctgatctcaa tgccttgctt ttaaataaag cggaattatg ccctaacatt
      481 aaattacact ttaatatgaa attgcactct cttgatattc ataataaaaa aattaaattt
      541 gaaaataaaa atggtgattt tgttgaggcc tcatatcata gactaattgg agcagatggt
      601 gcgccgtctc atgtaaggga tatgctgaaa aatgaaggga tagttagtgc aagcagagat
      661 tttttatctc atggatacaa ggagctttca attagcaaaa aacatacaaa agggatggct
      721 agggagcatt tgcatttatg gccacgtgat tcgtttatgc tattaggtaa tcctaatcct
      781 gacgattcaa ttacaggttc tttgttttta gctaatgaag ggaaagacag ctttgctgaa
      841 ctaaataatg aagaaaaatt acatttattt tttaaaacac agttcccgga tgcctatgca
      901 gctatgccaa atttggtcca agaatttttt ggtaatccta ctggacattt gagtactatc
      961 caatgctcac cctggtacta taaagacgaa tgcttattaa taggtgatgc ggcacatggg
     1021 ataattccat tctttgggca aggaatgaat agcgcatttg aagattgccg tattcttgat
     1081 gaattactgg atgaatacca agacgactgg tcgcgagtga accctctttt ttatgaacaa
     1141 agaaaagtga ataccgatgc gatagcgaaa atgtccatgg ataattatca tgagattcat
     1201 tcggacataa gaaatccaaa atttatttta caaaagcaga ttgaacggga actcatgttg
     1261 cgatacccgg aacattatgt ttccatgcat gttttggtta tgtttaccaa taccccgtat
     1321 gctaaagcga tggcaatagg cgagttacaa tctggccttt tagagcaaat ttgtttccca
     1381 ataactgata ttaaggagtt aaattggcag gaagtagaga aattacttgg catatatgac
     1441 aaaaaattgg cgaaaattat ttgatttaat ttatgtcatt cattttaaat gaaatttata
     1501 gtcaaagttt tgtcagaaaa gatggattca gggacatttt tttgtcgcta cctgagggtt
     1561 attagcgcaa agaataaaaa aataacttaa tttttgttgt attagtgagc attttgacca
     1621 aaaagatggc acgccttctg cagtataaac ttgtct
//
LOCUS       DAXLDT010000313         1583 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXLDT010000313 DAXLDT010000000
VERSION     DAXLDT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.314"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1464
                     /locus_tag="ACF2SP_003168"
     CDS             <1..1464
                     /locus_tag="ACF2SP_003168"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFD2360912.1"
                     /translation="ALSRASYPEALLADIEAIATGTLAHTPAVLTPSRITDDASSLYA
                     TRLLASTPDAPESAIRAVVENPGFNPDAKDNNRLTPFYLAAAYGHIPLASVLLERGAN
                     YLVKCLNQYSSLYIAVENNHLPFLRWFLGALSVEQRIELLVVQDQHRNTALHKALSRP
                     ELLKLLLEGLSLESRVQLLEMKSQYGNTVLHLAASRPESLKFLLEGLSIEQRVELLTT
                     LQRKDGASVLHFATSNPESLKLLLEGLSIEQRVKVLKMKNQYGESVLHIVASYPEPLK
                     LLLEGLSIEQRVELLAVQGQVGNSVLHEALSQPQSLKLLLDGLSEEGRVQLLEMKNQY
                     GNTVLHLAASHSEPLKLLLEGLSEEKRFELLRAKNQHGYSVLYRALSCYPESLGVILE
                     MLCETTQRQVLEDLGCLRGNVFLGKPEILDEINFALKFSVSVSNTSVSCLDTSSQAAT
                     SSGNQNTFFDSGSKRRIDAQRNTSPEETEERACSLKK"
BASE COUNT          440 a          369 c          352 g          422 t
ORIGIN      
        1 gcgctttcgc gtgcctccta tcccgaagca ctgcttgcag acattgaggc cattgccaca
       61 ggcactttag cacacacacc agcagtactt actccgtcgc ggataaccga tgatgcatcc
      121 agtctttacg cgacaagatt acttgcttca acacctgatg caccagaaag cgctattcga
      181 gccgtggttg aaaacccggg atttaatccc gatgccaaag acaacaaccg tcttactcca
      241 ttctaccttg ctgcagcgta tggccatatc ccgctggcaa gcgttcttct cgaacggggt
      301 gcgaattatc tggtgaagtg tcttaaccag tattcctcgt tgtacattgc agtagaaaat
      361 aatcatctgc cttttttacg atggttcctt ggcgcattgt ccgtggagca acgaatcgaa
      421 ctgcttgtgg tgcaggacca acatcgcaac actgcactgc ataaggcttt atctcgcccc
      481 gaattactga aactcctttt ggagggtttg tctttagaga gccgagtcca actgctggag
      541 atgaagagcc aatatggaaa taccgtactg cacttggctg catctcgccc cgaatccctg
      601 aaattccttt tggaaggctt atctatagag caacgagtcg aactgcttac tacgttgcag
      661 agaaaagatg gtgcaagtgt actgcacttt gctacatcta accccgaatc cctgaaactc
      721 cttttggagg gcttatctat agagcaacga gtcaaggtgc tgaaaatgaa gaaccaatat
      781 ggcgaatccg tactgcacat cgttgcatct tatcccgagc ctctgaaact ccttttggag
      841 ggcttatcta tagagcaacg agtcgaactg cttgcggtgc agggccaagt tggcaatagt
      901 gtactgcacg aggctttatc acaaccccaa tccctgaaac tccttttgga tggtttatct
      961 gaagagggcc gagtccaact gctggagatg aagaaccaat atggaaatac cgtactgcac
     1021 ttggctgcat ctcattccga gcctctgaaa ctccttttgg agggtttatc tgaagagaaa
     1081 cgatttgaac tgcttagggc gaagaaccag catggctatt ccgtattgta cagggcttta
     1141 tcatgttatc ctgaatcttt aggggtgatt ctagaaatgc tgtgtgagac aactcagcgg
     1201 caagtgctcg aggatttggg atgtttgcgt ggtaatgtat tcttaggtaa gcctgaaatc
     1261 ctagatgaaa taaactttgc cttgaagttt tctgtatccg tcagcaacac ctcagtatct
     1321 tgccttgata catcatcgca ggcagcaact tcatctggca atcaaaacac gtttttcgat
     1381 agcggttcca aacgcagaat cgatgcacaa agaaatacat cgccagaaga aactgaagaa
     1441 agagcatgct ctctcaaaaa ataaacgttg acacttaaat acaggttgca ttgtaaagaa
     1501 aaattaattg caacctgata gatatatttg ccagaataga tatatttatt taagtgaaaa
     1561 tacgtacagg gtcagatctt act
//
LOCUS       DAXLDT010000314         1572 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXLDT010000314 DAXLDT010000000
VERSION     DAXLDT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.315"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(25..1320)
                     /locus_tag="ACF2SP_003169"
     CDS             complement(25..1320)
                     /locus_tag="ACF2SP_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HFD2360913.1"
                     /translation="MIGDKSVLDVYLGESQIANITLIDDQLYWSYHESWQQTGYAISP
                     HLPMHEYIPPLNVQRFLRNLLPEGNPLEVLVNVFHLSKYNTFGLIQALGLDTPGSLVI
                     LPSNQAIPKHANFRLVTHEELEERLNHRDSYNLIVWDGKPRLSVAGVQDKINVVLNKE
                     GQLGFGEGSLCSTHILKFETQKLSYLVLNEFLSMWLAKCCELNVANVQMKRFGQNPAL
                     LVERFDRKLITTNIVKRRHLIDGCQAMNLPPEFKYEQNFGNSRDVAHVRDGVSLPKLF
                     DFANQCINPAKTKQQILDWVLFNVLIFNCDAHGKNISFFVGANGISLAPFYDLVNIKM
                     YPEFEHALAMALGDEFDGYNINAYQLADFADTCRLPRSLVAKHLKYLIGKLTTALQEE
                     TQFNLVGNEEGYFKKYQEIVMKRCEHLLKQNDQITSIKL"
     gene            complement(1307..>1572)
                     /locus_tag="ACF2SP_003170"
     CDS             complement(1307..>1572)
                     /locus_tag="ACF2SP_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360914.1"
                     /translation="KLKTPNLQQPLTAELLGEVIKARRTQSNLRLEDAAALCGVAKET
                     FMKIEHGQSNCQLASVLQICSGLGISLYIKPWMDNSEDENDWR"
BASE COUNT          500 a          316 c          248 g          508 t
ORIGIN      
        1 agtaagaaat agtgaggaaa ggcgctagag ctttattgaa gtaatttggt cgttctgctt
       61 taacaagtgc tcacatctct tcatgacaat ttcctgatat ttcttaaaat agccttcttc
      121 gttaccaact aaattaaatt gggtttcttc ttgcaaagca gttgttaatt tgccaatcaa
      181 atattttaaa tgtttagcta ctaacgacct tggcagccga catgtatctg caaaatcagc
      241 caactgatag gcattaatat tatatccatc aaattcatcc cctaatgcca tcgccagagc
      301 atgctcaaat tcaggataca tttttatatt aactaaatca taaaatggtg ccagtgaaat
      361 accattagcc ccaacaaaaa aactgatatt tttaccgtga gcgtcacaat taaatatcaa
      421 aacattaaat aaaacccagt cgagtatttg ctgcttggtt ttggcaggat tgatacattg
      481 attggcaaaa tcaaatagct tgggcaaact gactccatct ctaacatgag ccacatccct
      541 gctatttcca aaattttgct catatttaaa ttcagggggt aaattcattg cctgacaacc
      601 atctattaaa tgccgtcttt ttactatatt agtggtaatc aatttcctat caaaacgctc
      661 taccaataaa gcagggttct gaccaaaacg cttcatctga acattggcta cattcaattc
      721 acagcattta gcgagccaca tactgaggaa ttcatttaaa accaaataag atagtttttg
      781 tgtttcaaat ttaagaatat gggtagagca caagctacct tctccaaaac caagttgtcc
      841 ttctttattt aacaccacat taattttatc ttgtactccg gcaaccgaaa gacgtggttt
      901 tccgtcccaa acgattaaat tgtaagaatc acgatgattt aaacgctctt ctaattcttc
      961 atgagttact agtcgaaaat ttgcatgttt aggaattgct tgatttgatg gcaatataac
     1021 taatgaccca ggcgtatcca accctaaagc ttgaataagt ccaaatgtat tgtatttact
     1081 taaatgaaaa acattaacta gtacttctaa aggattccct tcgggtaata aatttcttag
     1141 aaatcgttgc acatttaatg gtggaatata ttcatgcata ggaaggtgtg gggaaatagc
     1201 ataccctgtc tgttgccaac tttcatgata actccaatac agttgatcat cgataagtgt
     1261 gatattggct atttgacttt caccgagata aacatccaag actgacttat ctccaatcat
     1321 tttcatcctc actattatcc atccaaggtt ttatgtaaag acttattcct agaccagaac
     1381 aaatttgtaa tacacttgcc aattgacagt tcgattgccc atgctcgatt ttcataaagg
     1441 tttctttggc aacaccacat aaagcagccg catcttctaa ccgcaaattg ctttgggttc
     1501 gacgtgcttt aatgacttcc cctaacaatt ccgcagttaa tggttgttga aggttaggag
     1561 tctttaattt tt
//
LOCUS       DAXLDT010000315         1526 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXLDT010000315 DAXLDT010000000
VERSION     DAXLDT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.316"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..432
                     /locus_tag="ACF2SP_003171"
     CDS             <1..432
                     /locus_tag="ACF2SP_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFD2360915.1"
                     /translation="QKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAK
                     PGTIKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMP
                     GDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE"
     gene            494..569
                     /locus_tag="ACF2SP_003172"
     tRNA            494..569
                     /locus_tag="ACF2SP_003172"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:527..529,aa:Trp,seq:cca)
     gene            583..954
                     /gene="secE"
                     /locus_tag="ACF2SP_003173"
     CDS             583..954
                     /gene="secE"
                     /locus_tag="ACF2SP_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFD2360916.1"
                     /translation="MKSSNENQGKMKDIMSWLAIILATAGAFVCTYYYTFTGPIQSII
                     WLAWLILILFLGYLTTTGKRVFVFAQEAKVELLKVVWPTRQETIQTTTIVMVMVGLTG
                     FILWGVDSIMMWAIAKLTHLG"
     gene            957..1505
                     /gene="nusG"
                     /locus_tag="ACF2SP_003174"
     CDS             957..1505
                     /gene="nusG"
                     /locus_tag="ACF2SP_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFD2360917.1"
                     /translation="MVEENKAKQWYVVHAYSGYENFVMREITSRAKHHNLEDKIGEVV
                     VPSEEVVEMRSGQKRKSTRKFFPGYVLVHMVMDDQTWHMVRGIPRVLGFIGGTSQTPT
                     PISDKEAQAIMQRVEDGVTKPRPKILFEPGEVIRVKEGPFVDFNGVVEEVNYEKNRLR
                     VAVLIFGRSTPVELEFSQVEKT"
BASE COUNT          502 a          255 c          365 g          404 t
ORIGIN      
        1 caaaagacga cttgtacggg tgttgagatg ttccgtaaat tacttgatga aggtcgagct
       61 ggtgataacg ttggagtgtt attacgaggt acgaagcgag atgaagtgga gcgtggacag
      121 gtattggcaa agccaggtac catcaagcca cacaccaagt ttgaagcaga agtgtatgtg
      181 ttatccaagg aagaaggcgg acgtcacaca ccattcttta atggataccg tccacaattc
      241 tatttcagaa ccactgacgt gacaggtact tgtgacttgc catcaggagt tgaaatggta
      301 atgcctggag ataatgtgca attagttgtt agcttgcatg ctccgattgc gatggatgaa
      361 ggtttaagat tcgcaattag agagggtggc cgaactgttg gcgccggtgt agtcgctaaa
      421 ataatcgagt aaaataggaa aaagtacaaa agtggcatga gccttatata catggctcat
      481 gtcaataaaa tttaggccag tagctcaatt ggcagagtgg cggtctccaa aaccgcaggt
      541 tgggggttcg attccctcct ggcctgccaa ctacaagtaa atatgaaaag ttcaaacgaa
      601 aatcaaggta agatgaaaga tataatgtcc tggctagcta tcatattagc tacagcagga
      661 gcttttgtat gtacttacta ttatacgttc acaggaccaa ttcaatcgat aatatggctt
      721 gcttggctta ttttgatcct tttcttgggt tacttaacaa caacaggtaa aagagtcttt
      781 gtgtttgcgc aagaagcaaa agttgaatta ttaaaagtgg tttggcctac acggcaagaa
      841 acaatacaaa caactactat agtaatggtg atggttggat tgacaggatt tatactctgg
      901 ggagtagatt caatcatgat gtgggcaatt gctaagttaa cacatttagg gtgaatatgg
      961 tcgaggaaaa caaagcaaaa cagtggtacg ttgtacatgc atattcagga tatgaaaatt
     1021 ttgtcatgcg cgaaataaca tctcgtgcaa aacaccacaa tttagaagat aaaataggcg
     1081 aggtagttgt accttcagaa gaagttgttg aaatgcgttc aggccaaaag cgtaaaagta
     1141 cccgcaaatt tttcccagga tatgtactcg ttcatatggt aatggatgat caaacatggc
     1201 acatggtcag aggcataccc agagttctag gctttattgg aggaaccagc caaactccaa
     1261 ctccgatatc tgataaagaa gctcaggcca ttatgcaacg agttgaagat ggcgtaacta
     1321 agccaaggcc aaaaatacta tttgagcctg gagaagtaat cagagtgaaa gaaggtccat
     1381 ttgttgactt taatggagtt gttgaagagg tcaattatga gaaaaacaga ctaagagtcg
     1441 cagttcttat ttttgggcgt tcaactccag tagaacttga gttcagtcaa gtagaaaaaa
     1501 cataaatgat ttagataaaa tttaaa
//
LOCUS       DAXLDT010000316         1510 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXLDT010000316 DAXLDT010000000
VERSION     DAXLDT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.317"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1510
                     /locus_tag="ACF2SP_003175"
     CDS             <1..>1510
                     /locus_tag="ACF2SP_003175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HFD2360918.1"
                     /translation="GTISTSWGTAGNWSSGIPGLPVGVATDKATFTGTTPFTTVANNN
                     VVRTLATLEFSAGATQYTITNLTAGGAFTLSGQGIVNNSANTQIITNTQSFTFTNLAD
                     AGTNVLFNNNAGGTLTFLGNSTATNTTIANAGALNISGLTASEISIGSLSGTAGTINL
                     GSKKLKTGGLNTSTTVASVISGVGGVLEKEGSGTLTLTGANTYTGGTIISGGTLQIGA
                     GGTVGSIAGNILNNAALIFNRSNALTYAGVISGTGTLTKLGAGVLTLTGNHTFSGDTT
                     ISAGTLSIGSGGTTGSIVGNIIDNSALIFNRSDNLTYAGVISGSGTLTKQGSGVLSLT
                     GTNLYTGTTTITAGTLSIGDGGILGSVAGNITNSATLRFNRADNLSYGGVISGTGALQ
                     KQGAGTLTLSGNNTYSGTTTISGGTLLLTGNKSGAGTTTVNATTNLQIGNGGVTGLIA
                     GNITNNGTVAFNRSDAITYVGVISGSGVLQQLGSGVLTLIGANTFTGGTTISS"
BASE COUNT          433 a          292 c          360 g          425 t
ORIGIN      
        1 ctggaacgat aagcacttct tgggggactg caggtaattg gagttcaggc attccaggtt
       61 tgccggtcgg tgtcgcaacg gataaagcga cgtttacagg aacgacacct tttacaacag
      121 tggctaacaa taatgttgta cgaactttag caactcttga gtttagtgcc ggtgcaactc
      181 aatacacgat tacaaattta accgccggcg gcgcatttac cttaagtggt caagggattg
      241 tcaataattc agcaaatact caaatcatta caaatactca aagttttact tttacgaatt
      301 tagcagatgc cggaactaat gttttattta ataacaacgc gggtggaacg ctcacttttt
      361 taggtaatag caccgcaacc aacacaacta tagctaatgc gggagcattg aatatttcag
      421 ggctgactgc cagtgagatt tcaataggga gcctttcagg cactgcagga acaattaatc
      481 taggttctaa aaaactcaaa acaggtggtt taaatacttc aaccacagtt gcttcggtga
      541 tttcaggggt tggtggtgtt ttagaaaaag aaggctcagg tactttaacg ctaacaggag
      601 caaatactta cacgggcgga actattattt caggaggaac tttacaaatt ggcgctggag
      661 gtacggttgg aagtatcgcg ggcaatatct taaacaacgc agcattaata tttaatcgtt
      721 caaacgcctt aacttacgca ggtgtgattt caggcactgg aacactgacc aagttagggg
      781 caggagtact tactttaaca ggaaatcata cctttagtgg ggatacgacc atcagcgcgg
      841 gtactttaag catcggcagt ggcggcacta ccggcagtat tgttggcaac atcattgata
      901 attcagcatt gatttttaat cgttccgata atcttactta tgctggagtc attagcggat
      961 caggtacctt gacaaaacaa ggttctggcg tgctcagttt gactgggact aatctttata
     1021 cgggtacgac taccattaca gccggtacct taagcattgg tgatggggga atcttgggca
     1081 gtgttgcagg aaatatcacc aatagtgcca ctcttcgctt taatcgcgca gataatttaa
     1141 gttatggtgg tgtgattagc ggtactggtg ccttacaaaa acaaggtgca ggcacactaa
     1201 ctctttctgg gaataatacc tactctggga caacgaccat ttcaggagga acacttcttt
     1261 taacgggaaa taaatccggt gcaggaacaa ccaccgtcaa tgccacaact aatttgcaaa
     1321 ttggtaatgg tggtgtgacg ggattgattg ccggtaatat tactaataat ggtacggttg
     1381 cttttaatcg ttctgatgcg attacttatg tcggtgtaat aagtggttct ggggttttgc
     1441 agcaattggg ttctggggtt cttactttaa taggggcgaa tacgtttact ggaggaacaa
     1501 ccatttcctc
//
LOCUS       DAXLDT010000317         1483 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXLDT010000317 DAXLDT010000000
VERSION     DAXLDT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.318"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..835)
                     /locus_tag="ACF2SP_003176"
     CDS             complement(<1..835)
                     /locus_tag="ACF2SP_003176"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD2360919.1"
                     /translation="MSKSGEKYKPADEAFSRLSKHQQHLLKSTVPIQIIISDNASSDN
                     SLALLEQSKLNKQTITIHRNNSNYGFSYANNAVFTKISTPFILYLNPDCFVENDTIEN
                     LLHTMHQHNDAGMVGCLVNNMDGSVQIGCCGLTPTPARVLNQLLKLSKIFPNNPKFGG
                     YLFSKEKLPTKPAEVELISGSCMFVRKKAIDEVGLLDDNYFLYCEDYDWFYRFTQSNW
                     KIIFNPNTKVTHIKSFSTKQIPFKVLLYKAKGMWRFYNKFFKENSSPATTLIIRVGII
                     AR"
BASE COUNT          472 a          253 c          252 g          506 t
ORIGIN      
        1 atctcgcgat aattccaact ctaataataa gagtggtagc tggagaagaa ttttctttaa
       61 agaatttatt ataaaatctc cacattccct ttgctttata caacagtact ttaaatggaa
      121 tttgtttggt ggagaaactt tttatatgag tgacttttgt atttggatta aaaataattt
      181 tccaattgct ttgtgtaaat cgataaaacc aatcgtagtc ctcacaatat aggaaatagt
      241 tgtcatccag aaggccaact tcatcaatag cctttttgcg tacaaacata caagaccctg
      301 aaatcaactc tacttcagct ggttttgtcg ggagcttttc ttttgaaaat aagtaccccc
      361 caaatttagg gttattcggg aagatttttg atagtttcaa tagttgattt aaaaccctgg
      421 ctggggttgg agtaagtcca caacagccaa tttgcacgct accatccatg ttatttacca
      481 gacatccaac cattccagca tcattgtgtt ggtgcattgt atgcaaaaga ttttcaatgg
      541 tatcgttttc aacgaaacaa tctgggttaa gatagagtat aaatggggtt gagattttgg
      601 taaaaactgc attattggcg taagaaaatc catagttgga attatttctg tgaatagtaa
      661 tagtttgttt atttaattta gattgttcta ataacgctaa actattatca gaagaggcat
      721 tatctgaaat aataatttga attggaactg tgctttttag cagatgttgt tggtgtttac
      781 ttaatctcga gaaagcttca tctgctggct tatatttttc ccctgatttt gacaggggta
      841 ttctgtttta tcctcatata gaaaggaatg ctgtccctgt atcaaaagga tgtacgtgct
      901 ttgttggaga attctctatt caaaagaacc acccatattc gatgcctacc aaattgtttc
      961 acgaacctct gtaacagggc aatttgtact ttgcttgttg agttactcgc tttattgagt
     1021 aaacgcgaat agataggaat tgatatgcat tcataacaga tagacagcat gaaaatattg
     1081 attttacacc tttcccaata caaattggtt ccgtctattt aaagatagag cttatcggat
     1141 gaactgaaaa ataaaaaata gctatcgagc tataaaacca aaatagattt caaaatcgcg
     1201 aaaaaaacgt tgtattctct tctatcgaga ttcaatctgt gcctcagatg gcatcagtac
     1261 tgcaattttt ttaaaataga tagtttttgc cactaaaaag ccaaccaatg ctcgtaagaa
     1321 aaaattaatt caagccttgt gccaatcgaa atgttcgcct aacatgcgag tttaactcgc
     1381 tgatattcct tatttatacc tcttttttcg cgaaacgaag tataaattat ttgaattaaa
     1441 tcagctaatt atcccttcgt atctttctaa gcccttaatc aat
//
LOCUS       DAXLDT010000318         1467 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXLDT010000318 DAXLDT010000000
VERSION     DAXLDT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.319"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..769
                     /locus_tag="ACF2SP_003177"
     CDS             <1..769
                     /locus_tag="ACF2SP_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFD2360920.1"
                     /translation="YLAGLLAFQSSGWLRLIYIFMILLTSYQVLFINTGRTGYLVYFI
                     LMLLLLIQKLNFKQALAGIVLFSGIIALAYHHSPVMQTRIYDLIQDIKLLKQHNKNTS
                     IGYRMQFHHYAKQLMLKHPFIGIGTGGFKYSYSQDVPVPSWGKELTDPHSQYWMTLAE
                     QGLIGLVLLIIFLASLFITSFQLTSSRPILLGVLFSFCIGSISDTILCYSTAGYLLVV
                     MSALCFGELIEKRRVKDAKEDHPALLSAHAPAAISQT"
     gene            complement(770..1105)
                     /locus_tag="ACF2SP_003178"
     CDS             complement(770..1105)
                     /locus_tag="ACF2SP_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurogenic locus notch"
                     /protein_id="HFD2360921.1"
                     /translation="MNILKPKAFLLASVFVLSISPAFAADGCCSKMGGINYCDSSAGR
                     LVCNNGFYSTCYCTRHAVMDLQFLMGCCLWHGGVYPQLNSSGLVVCNDGYVSEECSLQ
                     KPVEQISVY"
     gene            complement(1275..>1467)
                     /locus_tag="ACF2SP_003179"
     CDS             complement(1275..>1467)
                     /locus_tag="ACF2SP_003179"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFD2360922.1"
                     /translation="IYQTGTLTTIILGLLSLYYFAGIFLGIFPGEKGVSWQGHLFGLL
                     AGFLTSYLLEIYPQILIYY"
BASE COUNT          475 a          246 c          283 g          463 t
ORIGIN      
        1 ctatttagca ggattgctgg cttttcaaag ttcgggctgg ttgcgactta tttatatctt
       61 catgatatta ctaaccagtt accaagtgct ttttataaat actggaagaa cgggttattt
      121 ggtctacttt attttgatgt tattgctttt gatacaaaaa ttaaacttca aacaggcttt
      181 agcagggatt gttctcttca gcggcatcat tgctttggca tatcatcata gccctgtaat
      241 gcaaaccaga atttatgatt taattcagga tatcaagcta ttaaaacaac ataataaaaa
      301 tacatcgata ggctatagaa tgcagtttca tcactatgcc aagcaattga tgttgaaaca
      361 tccttttatt ggaataggca caggtggatt taaatatagt tattcacaag atgtacctgt
      421 tccaagttgg ggtaaagagt tgacagatcc acacagtcag tattggatga ctttggctga
      481 gcaaggttta attggtctgg tacttttgat aatattttta gcgagtttgt ttattacatc
      541 atttcaattg actagctcca gacctattct tttaggtgtt ttattttctt tttgtatagg
      601 ctccatttcc gacacgattt tatgctattc gactgcaggt tatttgttgg tagtgatgag
      661 tgcattatgt tttggtgagt taattgagaa gcgtagagtc aaggacgcaa aagaagacca
      721 tcctgctctt ttatcagccc atgctccagc agcaataagt cagacctaat caataaacag
      781 aaatttgctc aacaggtttt tgcaatgaac attcttccga aacatatccg tcattgcaaa
      841 ccacaagacc cgatgagttt aattgtggat agaccccgcc atgccataag caacatccca
      901 taagaaattg taaatccatt acggcatgac gtgtgcagta gcaagttgaa tagaaaccgt
      961 tgttacaaac aagacgacca gcagaagaat cgcaataatt tatacccccc attttactgc
     1021 aacaaccatc agcagcgaaa gcaggggata ttgataaaac aaacacactg gctagtaaaa
     1081 atgcttttgg tttcaatatg ttcattgtgg ttctctgatt ggtttttatt tttaagaata
     1141 gcatagagtt agatggatta ttagtgaaat atgatgattt aattgcagtt taagactgaa
     1201 tttattagga caaaaaagtt caaagtgaaa tgataaattg gtttcatggg ctagctattg
     1261 tgaagagtta atgactaata ataaatgaga atctgaggat agatttctaa taaatagctg
     1321 gtgagaaaac ctgctaatag cccaaacaag tggccttgcc aggatacgcc tttttcacct
     1381 ggaaaaattc ccagaaaaat accagcaaaa taatacaggc taagcagacc aagaataata
     1441 gtggtcaagg tacctgtttg ataaatg
//
LOCUS       DAXLDT010000319         1447 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXLDT010000319 DAXLDT010000000
VERSION     DAXLDT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.320"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            176..502
                     /locus_tag="ACF2SP_003180"
     CDS             176..502
                     /locus_tag="ACF2SP_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HFD2360923.1"
                     /translation="MTKKSLFKISFANQDEIYEIYARSIKESDMFGFLEVEELVFGEQ
                     TALVVDPSEEKLKMEFCDVKRIFIPVHSIFRIDEVSKQGTSKVKDNLSHGNKVRPFPG
                     AGKIKD"
     gene            545..1114
                     /locus_tag="ACF2SP_003181"
     CDS             545..1114
                     /locus_tag="ACF2SP_003181"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFD2360924.1"
                     /translation="MTIPDKIKAVYEKSTCLYTSNEVEAALDRMAIKIHETLQDKNPV
                     IICVMVGGLVPLGNLLHRLDFPLEVDYVHATRYRGDLTGGDILWKVRPSSNLAGRTVL
                     VVDDILDGGITLAAIINEIKAMGAAEVYSAVLVDKYRKRVPNGLQKADFVGLQVEDHY
                     IFGYGMDYHEYLRNAPGIFIVHPDHEANK"
     gene            complement(1116..>1447)
                     /locus_tag="ACF2SP_003182"
     CDS             complement(1116..>1447)
                     /locus_tag="ACF2SP_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFD2360925.1"
                     /translation="LSELKGMGIQISIDDFGTGYSCLSYLKDLPINVLKIDQIFVKDL
                     KNNQSNQTLIKAIIAMAKSFNLSLIAEGIQNKHELVFLQNLGCHYGQGYYFYHPMPPE
                     KCTQLLQ"
BASE COUNT          477 a          220 c          279 g          471 t
ORIGIN      
        1 ttatatttca ttaatattcc acatacgaaa gaggttaaat tattgtcacc attaagtcta
       61 aatgaataaa tttggagcta acaataagct ctagacgact aaactgcttt aaatatttga
      121 agatataccc ttactttttg tatactttat cctgctttta gataaggtta gatacatgac
      181 aaaaaaatcg ctattcaaaa tcagttttgc taatcaggac gaaatttatg aaatctatgc
      241 tcgttcaata aaagaaagcg atatgtttgg ttttctggag gttgaggagc tggtttttgg
      301 agagcaaacg gcactggtag ttgatccgtc tgaagaaaaa cttaaaatgg aattttgtga
      361 tgttaagcgc atttttattc ctgtacattc catatttcgc attgatgaag tttcaaaaca
      421 agggacatca aaagtcaaag ataacttgag ccatggcaat aaagtaaggc ctttccctgg
      481 agcagggaag ataaaagatt aacttcgttt agcctgtata aacaacttct aaggaaataa
      541 ataaatgacc attcctgata aaattaaagc tgtatatgaa aaatcaacct gtttatacac
      601 atctaatgaa gttgaagcag cactggacag gatggcaata aaaattcatg aaacactaca
      661 agataaaaac cctgtaatta tttgcgtgat ggtaggaggt ctagttccat taggtaatct
      721 attgcatcgt ttggattttc ctctagaggt ggattatgtt catgctaccc gttaccgagg
      781 tgatttaacg ggaggggaca ttctttggaa agtgagacca tcaagcaatc ttgcaggacg
      841 aacagtcctg gttgtagatg acattcttga tggtggtata accttggctg ccataattaa
      901 tgaaatcaaa gccatgggtg ctgcggaagt ttatagtgct gtattagttg ataaatatcg
      961 taaacgtgtc cccaatggtt tgcaaaaggc tgattttgtc ggtttacaag tagaggatca
     1021 ttatattttt ggttatggta tggattacca cgaatattta cgtaatgcgc cagggatttt
     1081 tatcgttcat ccagatcatg aggcaaataa ataaattatt gtaaaagttg cgtacatttt
     1141 tcaggtggca tggggtgata aaaatagtac ccttgaccgt aatgacaacc tagattttgt
     1201 aaaaagacaa gttcgtgttt gttttgaatt ccctctgcaa tcaaactcaa gttaaaactt
     1261 tttgccatcg caattatggc ttttattaga gtttggttgg attgattatt ttttagatcc
     1321 ttaacaaaga tttgatcaat ttttaaaaca ttaattggta agtcttttaa gtaacttaag
     1381 caagaataac cagtgccaaa gtcatcaatt gaaatttgga tacccattcc ctttaattca
     1441 cttaata
//
LOCUS       DAXLDT010000320         1439 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXLDT010000320 DAXLDT010000000
VERSION     DAXLDT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.321"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1070)
                     /locus_tag="ACF2SP_003183"
     CDS             complement(<1..1070)
                     /locus_tag="ACF2SP_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFD2360926.1"
                     /translation="MVYYINLKEQPLPQDKEILTQLIKRYLLPGSSQSALFAKHHYTL
                     NDENGNEIEFLLSHDIYKNQRHDGITEFLILNGPKIGSGTKGEVRQSNHAMYLMYDEL
                     VIFPKENVLKVLYRNFSNAEELIKQESVNYSILSGNSAAELVNLQGGSQGFFMQKEEG
                     CTLEELLEDIDSLQKLTFSDKMELAISFLTELENLHNKGFVHGDLTFKNILYNEESKR
                     LKIIDAGEMKVADERFLAQDILIARDMVSLLFTTNETNQFNASSYWSDEPTARRILYP
                     NLVEAKLNTILNSEYTCISALKLAIEMNYNEYLKHLNPVLKRNEFAKTESEASNLQAY
                     KDQMNKQQLLGEIRESKEVFLN"
BASE COUNT          451 a          249 c          205 g          534 t
ORIGIN      
        1 atatttaaaa aaacttcttt agattctcga atttccccca agagttgttg cttattcatt
       61 tgatccttgt aggcctgtaa attcgaagcc tcgctttctg ttttggcaaa ctcatttctt
      121 ttcaaaacag gattcaagtg ctttagatat tcattgtaat tcatttcaat ggccaacttt
      181 aaagctgaaa tacaagtgta ttcactgttc aaaattgtat ttaactttgc ttcgaccagg
      241 tttgggtaaa gtattcgtct ggctgtgggt tcatcagacc aataacttga tgcattaaat
      301 tgatttgttt cattagttgt aaacaaaaga gataccatgt ccctggcaat taaaatatcc
      361 tgcgctaaaa agcgttcatc tgctactttc atctctcctg catcaatgat ttttaatcgc
      421 ttactctctt cgttatataa aatatttttg aaagttaaat cgccatggac aaagcctttg
      481 ttatgcagat tttctaactc agttaaaaat gaaatggcta actccatttt gtctgaaaat
      541 gtcaattttt gaagtgagtc tatgtcctca agaagctctt ccaaagtaca cccttcttct
      601 ttttgcatga aaaaaccttg gcttcctcct tgcaaattga ctaattctgc agctgaatta
      661 cctgataaaa tcgaataatt gacagattcc tgttttatta gctcctcggc attgctaaaa
      721 tttcgatata aaaccttcag tacattctct ttagggaaaa ttaccaattc atcatacatt
      781 aaatacatag catgattact ctgtctcact tcgcctttgg ttccgctccc tatctttgga
      841 ccatttaaaa ttaaaaactc agtaatacca tcgtgacgtt gattcttgta tatatcatga
      901 ctcaagagga actctatttc atttccattt tcatcgttta gcgtgtaatg atgtttagca
      961 aacaatgcgg actgactgga accaggcaat aaatatcgct taattaactg cgttaaaatt
     1021 tctttatctt gaggtagagg ttgttccttc aaatttatgt aataaaccat aatgctctcc
     1081 tataagccaa tcatctgctt atatttgagc atattgatta atataaatac aattaattgt
     1141 tttgatatgt tttattaaat ttagttatat ctattatttt tcaatgaatt actgatgatt
     1201 tgacgtcttt ttatgagcag ttatttattt tgataaatat caaaaaatat tattaacaac
     1261 ccctattcat aataccgttt gttagaaaca aactgtttcc ttaactataa ttctattaat
     1321 aaagaaaaag atatggtcgg taaattccaa attgtagttt ttgggcttat tcagttaccc
     1381 tggataggaa tataaaaaag ttaatttgga tttaatgaaa aatggatctc agaaaaatt
//
LOCUS       DAXLDT010000321         1424 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXLDT010000321 DAXLDT010000000
VERSION     DAXLDT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.322"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..239)
                     /locus_tag="ACF2SP_003184"
     CDS             complement(<1..239)
                     /locus_tag="ACF2SP_003184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360927.1"
                     /translation="MHLVNSYRPILGGWHRLWIVFCVVSLAIIFVTNKLYVGLNPEYY
                     SFRNEFYEALSIESKNKIIEINVADELGALSNSFE"
     gene            complement(318..839)
                     /locus_tag="ACF2SP_003185"
     CDS             complement(318..839)
                     /locus_tag="ACF2SP_003185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360928.1"
                     /translation="MEIKILETKWFHRLVKVMYIFFLTVGIISVLCFGWSLKPYQLID
                     TEKSYLTCPDGTKHSFDSLDIYFYSKRSFSNYETAKAIGECNKHLKKEKLVTDPSILK
                     ILNKQGGNWEIVKGDPYPTDWIYKTKGSWVMAIKLWVFGSLIVFLIFNIIKQSLLYIV
                     YGKKFTLSCRKES"
     gene            complement(848..1213)
                     /locus_tag="ACF2SP_003186"
     CDS             complement(848..1213)
                     /locus_tag="ACF2SP_003186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360929.1"
                     /translation="MIKKLMDKLGYISKNDCFKMMNIDGKGYLESTKSSGCSTIPMLI
                     AWHLFEAVIELLENNNEQFKEENRPYIHLRNQGVIDPPTAFGLFMTYIVSHPDEHHLS
                     LFNLAIRCVKASEINSSEK"
BASE COUNT          456 a          257 c          201 g          510 t
ORIGIN      
        1 ttttcgaatg aatttgaaag agcaccaagc tcatctgcta catttatttc aataatttta
       61 ttttttgact ctattgagag agcttcataa aactcattcc ggaaagaata gtactctggg
      121 ttcagaccaa catataattt atttgttaca aaaatgatag caagacttac cacacaaaat
      181 acgatccata atcgatgcca tccccctaaa ataggacggt aactattgac taaatgcatt
      241 tttccccctt tgtttaacgt tgtatgtatt tttttgaata ggggcaactc caagaactat
      301 ataaatgaat ttatttttca agattctttt ctacaactta gagtaaattt tttgccataa
      361 acaatataga gcagggattg cttgatgatg ttaaatatca agaaaacaat caaagaacca
      421 aaaacccaca attttattgc cattacccag ctacctttag ttttataaat ccaatcagta
      481 ggataaggat ctcccttaac tatttcccag ttgccaccct gtttgttaag tattttaaga
      541 attgatgggt cggtaacgag tttttctttt ttcaggtgtt tgttacattc tcctattgcc
      601 tttgctgtct cataattaga aaagcttctt tttgaataaa aataaatatc taggctatca
      661 aatgaatgct ttgttccatc agggcatgtt aaataacttt tttctgtatc aatcaattga
      721 tatggtttta aactccaccc aaagcaaagc acacttataa ttcctaccgt taaaaaaaat
      781 atatacatta cttttaccaa cctgtggaac cacttggttt ctaggatttt tatttccatg
      841 tttttctcta tttttctgat gagtttattt ctgacgcctt gacacatcta atggcaagat
      901 tgaataaaga taaatgatgt tcatcaggat gactgacaat gtaagtcata aacaggccga
      961 aagctgttgg ggggtctata acaccctggt tacgcaagtg aatataaggt ctattttctt
     1021 ctttaaattg ttcattattg ttttctagca actcaattac tgcttcaaat aagtgccaag
     1081 caattaacat cggtattgtc gagcaaccac ttgatttagt tgactctaaa tatcctttgc
     1141 catctatatt catcatcttg aagcaatcat tttttgatat ataaccaagc ttatccatta
     1201 acttcttaat catttaaatt tcctcaatta acaaaattta gacaatagga accctctcag
     1261 gaatattcct gctaaaacaa gtccgtgttt atttttatta ttaagttgcg tatgagtctt
     1321 tatggcggtc gcttaatcag cataatttta ttattgagcc tattgaaatg ctcttttttt
     1381 gaacttcagt gcaataatac accaaaaatg gaaataatac cact
//
LOCUS       DAXLDT010000322         1419 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXLDT010000322 DAXLDT010000000
VERSION     DAXLDT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.323"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1279
                     /locus_tag="ACF2SP_003187"
     CDS             <1..1279
                     /locus_tag="ACF2SP_003187"
                     /inference="COORDINATES: protein motif:HMM:NF045532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lpg0008 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360930.1"
                     /translation="VNKEILMTFTCDELKGLEHPYEVLGNGDALAENREELNKLTNDE
                     KLVLASRLVLECPVNELKDFAHAIEAARMPQDDSDTFHSFLFQAYQVKKRIISLLDPR
                     NINPHSMILEKEFDGELFNNFNKLAIDVLTNNEVAIALRLAETTPAQDRSRVSQNINN
                     IFPQSLFAAKVGHAFAVRRDIERLLLGDRPDQFFSSREFKIDSCIEFASLFNVINDKE
                     SSIAGKLALRTPAENRTDVVMKIKGFCAEDSELAIKVQSAFALRRDIERNLLGDNPEQ
                     FFSSRDFSVDLCLEFAILFPELLKGHEQAIGEKLAKLDAKVRSDISRKLEMINGAAHE
                     QSSPFRIIASAMVPKEEPCLTKPTPVALPSTEEIIPKQESPTSLRNRNVLFKEQTEEH
                     KKKNSQMEPIVSDSEEEEEKQNCWTSFCGLFGK"
BASE COUNT          464 a          277 c          275 g          403 t
ORIGIN      
        1 agttaataag gagattttaa tgacgtttac ttgcgatgaa ttaaaaggat tggagcaccc
       61 ctacgaagta cttggcaatg gtgacgcact ggcggaaaac cgcgaagagc taaacaaatt
      121 aaccaatgat gaaaaattag ttttagccag ccgcctcgtt ttggagtgtc ccgttaatga
      181 attaaaagat tttgctcatg ccattgaagc agcaagaatg ccacaagatg atagcgatac
      241 ctttcattcc tttttattcc aggcttatca ggtaaaaaaa agaatcattt ccttgctcga
      301 tccgcgcaat ataaaccctc attccatgat attggaaaag gaatttgatg gtgagctatt
      361 taataatttt aataaattgg ctatcgatgt attaactaac aatgaagtcg caattgcatt
      421 gagactggct gaaacaactc ctgctcaaga tcgttcccga gtatctcaaa atatcaacaa
      481 tattttccca cagtctctgt ttgccgccaa agtcggccac gcttttgccg ttcgcagaga
      541 catagagcga ctattgctag gtgacaggcc tgatcaattc ttctcaagtc gtgaattcaa
      601 gatcgattct tgcatcgaat ttgcaagttt attcaatgta attaatgata aagaatcttc
      661 cattgccgga aaacttgcac tgagaacccc tgctgaaaat cgcactgatg tagtaatgaa
      721 aattaaaggt ttttgtgccg aggatagtga acttgcgata aaagtccaat ctgcttttgc
      781 attaagaagg gatatagagc gcaatctatt aggtgataat ccagaacaat ttttcagtag
      841 ccgagatttt agcgttgatt tatgccttga gtttgcaatt ttattcccgg aattattaaa
      901 aggccatgaa caagctatcg gagaaaaatt agctaagctg gatgctaaag tacgctcaga
      961 tatcagtaga aaactggaaa tgatcaatgg agcagctcac gagcaatcaa gccctttcag
     1021 aataatagcc agtgccatgg tgcctaaaga agagccttgc ctgacaaagc ccactcccgt
     1081 tgccttgcca tctactgaag aaattatacc aaagcaagaa tcaccaacca gtttacgcaa
     1141 caggaacgtt ttatttaaag aacaaaccga agaacacaag aagaaaaact ctcagatgga
     1201 acccattgtc tcagattctg aagaagaaga agagaaacag aattgctgga ctagtttttg
     1261 tgggttattt ggcaagtaaa gcagccatat cttttgccct tacttggtta agggcaactt
     1321 tacacttttt tacccattct ttacacatca tgtagcaata attcatgtta atatttctca
     1381 taaattcatt gacttattgc aatctgctcg agttaaagc
//
LOCUS       DAXLDT010000323         1401 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXLDT010000323 DAXLDT010000000
VERSION     DAXLDT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.324"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..969
                     /gene="proB"
                     /locus_tag="ACF2SP_003188"
     CDS             <1..969
                     /gene="proB"
                     /locus_tag="ACF2SP_003188"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFD2360931.1"
                     /translation="KNLGHQVILVSSGAVGSGRKIAKNSLGKTYGDTVGEKQLLASIG
                     QPELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLTELLKHQNIIPIVNEN
                     DSVAIEELMFTDNDELAGLIAAQINADYLILLTNVDGVFTSHPNDSESKLINTIDPSS
                     GWPNVSAVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVPKILNREAVGTII
                     LASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRNGNRVMSILPIGIEDFRGECKKG
                     DLIDILNSNNEKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQSHIL"
     gene            978..>1401
                     /locus_tag="ACF2SP_003189"
     CDS             978..>1401
                     /locus_tag="ACF2SP_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HFD2360932.1"
                     /translation="MNTDIANQLRVAKKATTDLNLIQSDTRTIILKTLAANLEKHIEN
                     IIQENQKDLSLMLEQDPRYDRLLLNKERILSLANDVRKVASLPNPLGVNLLEKSMPNG
                     LSIKKITVPLGVIAVIYESRPNVTIDIFSLCFKSGNVCI"
BASE COUNT          499 a          228 c          252 g          422 t
ORIGIN      
        1 aagaacctgg ggcatcaggt cattttagta agctctgggg ctgttggctc tggtcgaaaa
       61 atagctaaga actctctggg aaaaacatat ggcgatacag tgggagagaa gcaactcttg
      121 gcttcaatag gccaacccga actgatgaat ctctattcta gcttgtttaa aaatgaaaac
      181 attttagtat ctcagttatt acttaccaag caagacttcc atactagaca gcattattta
      241 aacattgccc gtttattaac ggaattgcta aagcatcaaa atattatccc tattgttaat
      301 gaaaatgatt cggtagccat tgaagaatta atgtttaccg ataatgatga attagcagga
      361 cttattgcag ctcaaattaa tgctgattat ctaatactct taaccaatgt cgatggtgtg
      421 tttacatccc atcccaatga ttctgaatca aaactcatta atacaattga ccccagttca
      481 ggttggccca atgtctccgc tgtaaaatca aaattaggcc gaggtggtat ggtatccaaa
      541 ctggatactg ccagaaaaat ggctggttta ggtatcaata cagtgatcgc aaatgtaaat
      601 gtagaatcca ttgttccaaa aattctaaat agggaagcag ttggaaccat aattcttgct
      661 agtaaaagaa aatccagtat aaaaagatgg attgctttta gtcatagaac ttccttacca
      721 tctatagtaa ttaatgataa tttaattacc ttattacgaa atggtaatcg tgtaatgagc
      781 attttaccaa tagggataga agattttcgt ggagaatgca aaaaagggga tttaattgat
      841 atccttaatt ccaataatga aaagatagga gttggaatcg ccaggtatga tttcttaaga
      901 ttaaaggact atcttagact ccaaaataag cctgcattta tacattacga tcaatcgcat
      961 attctatagg tatcaaaatg aatactgata ttgctaacca attaagggtc gctaaaaaag
     1021 caactactga tcttaactta atacaaagtg atacccgaac aataattttg aaaacattag
     1081 cggctaattt agagaagcac attgaaaaca taatacaaga aaatcaaaaa gatttaagtt
     1141 taatgcttga gcaagatccc agatatgatc gtttattgtt aaataaagag agaattttgt
     1201 cattggcgaa tgatgtgaga aaagtagcca gtttacctaa cccattagga gttaacctcc
     1261 ttgaaaaatc aatgccaaat ggtttgagta ttaagaaaat cacagtacct ttaggtgtca
     1321 tcgcagtcat ttatgagtca agacctaatg taactattga tatcttcagt ctatgcttta
     1381 aatctggaaa tgtttgcatt t
//
LOCUS       DAXLDT010000324         1386 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXLDT010000324 DAXLDT010000000
VERSION     DAXLDT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.325"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            37..>1386
                     /locus_tag="ACF2SP_003190"
     CDS             37..>1386
                     /locus_tag="ACF2SP_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360933.1"
                     /translation="MTLGKEELQTSPEPSSKEKPTIDLAKIIIACHDKKPIFAGQPDS
                     ELTNLISVKDENGLAYWLHFNSFIKYQLRKIIQSADSDTALSDELVKNVHLILTKHLK
                     DLEDKKKLTTYASSDFSTDEYMQLSQVYDSVRTKQKINALGAQDIEPILNAKNRRLRE
                     LGRSGQLTPIWCADYASQATARKLAKAIASLDVGGRKQYLYYHFNGNHTIGFDVERDK
                     SGVYKIFCFESAADPKHMEALDLLYKELAKKDLKFEIKSCQSQLQKDEFNCSVYNLAV
                     LSELGKYAHAFDYLPEQLEEEVSLKATKEIEIEKSITKKRKVKLENMDKITWVKLSDM
                     PTKVIAMGQSYQAMEQALKKSKDFDLDPAVFIQLHKKKYHYDQSEEQSTKYINQRRKH
                     IMDKLDVSIQPILKKSHSKFLERLPLLGLINEGKIPDFHKEITENKSMSLDEKMTYIE
                     "
BASE COUNT          507 a          255 c          260 g          364 t
ORIGIN      
        1 aattcacaaa tgatatattt cctgaaggag tgcatcatga ctttaggaaa agaggagtta
       61 caaacatctc ctgagccttc ctcaaaagaa aaacccacca ttgatctggc gaaaataatc
      121 attgcttgcc atgataaaaa accaatattt gcaggacaac cagattcgga gctgaccaat
      181 ttaatcagtg tgaaggacga aaatggcttg gcttattggc tacattttaa ttcatttatc
      241 aagtatcaat tgcgtaaaat aattcaatca gccgacagcg ataccgcact ttctgacgaa
      301 ttagttaaaa atgtacacct tattttaacc aaacacttaa aggacttgga agataagaaa
      361 aaattgacca cttacgcatc cagtgatttt tcaacggatg agtacatgca attaagccaa
      421 gtttatgact cagtccgtac taaacagaaa ataaatgctt tgggtgccca ggatatagaa
      481 cccatattaa atgctaaaaa ccgtcgcttg cgagaattag gacgctctgg ccaactgact
      541 ccaatttggt gtgctgatta tgcctcacaa gccactgcaa gaaaacttgc taaagccatt
      601 gccagtttgg atgtgggggg tagaaaacag tatttgtact accatttcaa tggcaatcat
      661 accatcggct ttgatgtaga aagagacaag tcaggggtct ataaaatatt ttgttttgag
      721 tcagcagctg accccaaaca catggaggcg ctggacttgt tgtataaaga actagctaaa
      781 aaagatttaa aattcgaaat caaatcttgt cagtctcaat tgcaaaaaga cgaattcaac
      841 tgttcggtat acaatttggc ggttcttagc gagctcggta aatacgcgca tgcttttgat
      901 tatttacccg aacaattaga ggaagaggta tcgctaaagg caacgaaaga aattgagata
      961 gagaaaagca ttacgaaaaa gagaaaagtc aaactagaaa acatggataa aatcacttgg
     1021 gtgaaactat ctgatatgcc cacaaaagtc atagcgatgg ggcaatctta tcaggccatg
     1081 gagcaagcac ttaagaaatc caaagatttt gatcttgacc ctgccgtttt tattcaatta
     1141 cacaaaaaga aataccatta tgaccagtcc gaggaacaat caactaaata catcaatcaa
     1201 agacgcaaac atataatgga taaactggat gtttcaatac aaccaatatt gaaaaaatcc
     1261 cactcaaaat ttttagaacg attgccgttg ttgggcttaa tcaatgaagg taagattcct
     1321 gattttcata aagaaattac tgaaaataag tccatgtcac ttgatgagaa aatgacttac
     1381 attgaa
//
LOCUS       DAXLDT010000325         1343 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXLDT010000325 DAXLDT010000000
VERSION     DAXLDT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.326"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(167..1012)
                     /locus_tag="ACF2SP_003191"
     CDS             complement(167..1012)
                     /locus_tag="ACF2SP_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFD2360934.1"
                     /translation="MKFPAFEPINLKNDIQSLEAIDLCLSKIYPDQNQARKFFSDASL
                     EELASSIRQHGIIQPILVKERDHNRNEYQIIAGERRWRAAKIVGLEKIPAIIRKYNKA
                     NGMAVSLIENIQREDLNPLEEAQAIQRLIEECYMTHGEVAETLGRSRTTVTNLLRLLT
                     LTDEVKEMLKMGLLEMGHARALLSLSSIQQAETAKIIINKSLSVRETEKLVQRLNMPE
                     DKQEFFLDPQFEQKTKDWMAQLSKKLSSKVNMHFNDGGKGRVVISFDSLEEADWLMAH
                     LKVEG"
     gene            complement(1014..>1343)
                     /locus_tag="ACF2SP_003192"
     CDS             complement(1014..>1343)
                     /locus_tag="ACF2SP_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HFD2360935.1"
                     /translation="INYTKKYAKKERTGFYPIPYFISALKYDYKFKEESKPDGSQEKE
                     SNEQIEWEKKLFALQAELNHWEKLSGYTKAGDNQEHVKNMQKFVLETREKLKQHYLEK
                     PIKMEAV"
BASE COUNT          370 a          272 c          194 g          507 t
ORIGIN      
        1 ttgccacact ttaccattcg ccgtagtcca atcgtctctt gaaggtaaaa tttggaggtc
       61 atttttttcc agtataccag taaaattata actacagact cgagggcaaa catcttctaa
      121 tttttccaca tagttatcca ctaattttgt gaataaaaaa atagccctac ccttccacct
      181 taagatgggc cattaaccaa tctgcttcct ctaaagagtc aaaacttatc accaccctac
      241 cttttcctcc atcattaaaa tgcatgttaa ctttggatga aagttttttc gataattgtg
      301 ccatccaatc tttcgttttt tgttcaaatt ggggatctag aaaaaactct tgtttatctt
      361 ctggcatatt taagcgctga accaattttt cagtttccct aacggataaa gacttattga
      421 taattatttt tgctgtttct gcttgttgta tactcgacaa acttaataaa gctctggcat
      481 gccccatttc tagtaatccc atttttaaca tttcttttac ttcatccgtg agagtaagca
      541 atctaagaag attagtaact gttgttcgcg atctacctaa agtttcagca acttcaccat
      601 gcgtcatgta acactcttct attaaccgct gtatagcttg tgcttcttcc aatgggttta
      661 aatcttctct ttgaatgttt tcaattaaag aaactgccat cccgtttgct ttattatatt
      721 ttctgatgat tgcagggatt ttttctaatc ctactatttt agctgcacgc caacgccgtt
      781 cacccgcaat aatttgatat tcattcctat tatgatctct ttctttgaca agaataggct
      841 gtataatccc atgctgtcga attgacgagg ccagttcttc tagtgaagca tcgctaaaaa
      901 actttctagc ctgattttga tctggataaa tttttgataa acacagatca attgcttcca
      961 aggattgtat gtcatttttt agattaattg gttcaaaggc aggaaatttc atattaaacc
     1021 gcctccattt taatgggctt ctctaaataa tgttgcttta atttttcccg ggtttccaga
     1081 acaaatttct gcatgttttt aacatgctct tgattatctc cagctttagt ataacctgat
     1141 agtttttccc aatgattaag ctctgcctgt aaagcgaata atttcttttc ccactcaatt
     1201 tgttcattag attctttttc ttgagaaccg tctggcttag attcttcctt gaatttataa
     1261 tcatatttta aagcactaat aaaatatgga attggataaa aacctgtgcg ctcttttttt
     1321 gcatatttct tggtatagtt aat
//
LOCUS       DAXLDT010000326         1328 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXLDT010000326 DAXLDT010000000
VERSION     DAXLDT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.327"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1290
                     /locus_tag="ACF2SP_003193"
     CDS             <1..1290
                     /locus_tag="ACF2SP_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFD2360936.1"
                     /translation="QNNNLFAQQIPNLPLYNQLIAYTIPDKNADDILRRYSEHHFTQP
                     LFPNYPLHYPLHPMQCKMPEKPYNIILIMVDSLRYDSLSAKYMPNTAQFAKRSWQFSQ
                     HMSAGNATQPGLFSLFYSIPSNYSTAVLEQKKSPILIDLLLQHGYQTKIIWSGSMAPL
                     PLDQTIYKKIANLNTNGAPIDDTGDKDRYSTREAIQFLTDPKTNNPFFLHIFYNAPHD
                     YCRYQSFPQLYKPAIEECVRIGMTNHVDPVPYYNRYLNTVTFIDQEISKVLGVIEKKG
                     YLKNSIIIITSDHGQEFNDNRQNYWGHTSNFTPIQLHVPLIIHWPGELARRFNYLTSS
                     YDIVPTILQRLFACKNPVSDYSIGYNLLIEGNRAPFLLVGSYINMGIFEADRATALET
                     SGRITITGMKAEPLDDAVPRMDVITQALDLMRKYYGK"
BASE COUNT          438 a          264 c          217 g          409 t
ORIGIN      
        1 caaaataata atttatttgc tcagcaaata cctaatttgc ccctttataa tcaactcatt
       61 gcctatacta tccctgataa aaatgctgat gatatattac gccgctacag tgagcaccat
      121 tttacacagc ctctttttcc gaattatcca ttgcattatc ccttacatcc aatgcaatgt
      181 aagatgcctg aaaaacctta taacattatc ctcataatgg tagattcact tcgctatgat
      241 tccctaagtg caaaatatat gccgaatacg gcacaatttg caaaaagaag ttggcagttt
      301 tctcagcata tgagtgctgg aaatgccaca caaccaggat tgttttcttt attttattcg
      361 attcccagca actactcgac ggcagtcctg gagcaaaaaa aatcaccaat tttaattgat
      421 ttattattac aacatggtta tcagacaaaa attatttggt ctggctctat ggctccactg
      481 ccattagatc agactattta taaaaaaatt gccaatctta atactaatgg agcgccaata
      541 gatgataccg gagacaagga cagatattcc accagagaag ctatccaatt tttaacagat
      601 cctaaaacaa ataacccatt ctttctccat attttttata atgcgccaca tgactattgt
      661 agatatcaaa gttttccgca gttatacaaa cccgcaatag aagaatgtgt acgaattgga
      721 atgacaaatc atgttgatcc ggtaccttac tacaatcgtt atttgaatac agtcacattt
      781 attgaccagg aaatttcgaa agtattaggg gttatcgaaa aaaaaggtta tttaaaaaac
      841 agcatcatta taattacctc agatcatggc caagaattta atgataatcg tcaaaattat
      901 tggggacata ccagtaattt tacccctatt caactacacg ttcccttgat cattcattgg
      961 ccaggggagt tagctcgtcg ttttaattat ctcaccagca gctatgatat tgtccctaca
     1021 atattacagc gtttatttgc ttgcaaaaat cctgtttctg actacagcat cgggtataac
     1081 ttgctaattg agggcaatcg agccccattt ctattggtag gaagttatat taatatggga
     1141 atttttgaag cagatcgcgc cacagcatta gaaacctccg gaagaattac aattactggt
     1201 atgaaggcag agcctcttga cgatgcggta cccagaatgg atgttattac ccaggcattg
     1261 gatttgatgc gcaagtatta tgggaaataa aagattgtct ttaaaaataa attggcaatt
     1321 aacctttt
//
LOCUS       DAXLDT010000327         1326 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXLDT010000327 DAXLDT010000000
VERSION     DAXLDT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.328"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            6..620
                     /gene="lem15"
                     /locus_tag="ACF2SP_003194"
     CDS             6..620
                     /gene="lem15"
                     /locus_tag="ACF2SP_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem15"
                     /protein_id="HFD2360937.1"
                     /translation="MIYKITLTDNNLAQAFMSIPQDVIALDLSNNLLGSKSCVDLLVN
                     IADCISPGVTSLDLSSNGLRLTQVSLVTPKHVTSLNLCFNPLHEISIDGLEWLKDSLA
                     HIQIIYLSYDTVNNMSREQRQAFRSVIPNIQTIILVDKNGKEIGPSHTAKIANLIREL
                     GGGNPEVPSLLNQCTFFVKTQGINIDKAHIPEELKKRIHDCNPL"
     gene            complement(678..932)
                     /locus_tag="ACF2SP_003195"
     CDS             complement(678..932)
                     /locus_tag="ACF2SP_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360938.1"
                     /translation="MNSYAAKVTPVEEHIAQTTSPQFMSIFFLVQILSILIGVACLFV
                     AFYILRPRKKDQMLIQDQFLLYLILGLFFIFSPFLLEYLR"
BASE COUNT          455 a          223 c          236 g          412 t
ORIGIN      
        1 taattatgat ttataagata acattaactg acaataactt ggcccaagct tttatgtcta
       61 tccctcaaga tgtaatcgct cttgatttga gtaataatct cctgggctca aaaagttgtg
      121 tagatttatt agtaaatata gcggactgta tatctccggg tgtgacttcg cttgatttga
      181 gtagcaatgg gctcagatta acgcaagtct ctttagttac acccaaacac gtgacttcac
      241 ttaatttgtg ttttaatccc ttacatgaaa tatctattga cggtttagag tggcttaagg
      301 attcgttagc acatattcaa attatttatt taagctatga tacggtcaat aacatgtcaa
      361 gagagcaacg acaggcattt cgatcggtaa ttcccaacat acaaacaatt attttggtcg
      421 ataaaaatgg caaagaaatt ggtccttcgc atacagcaaa aatcgctaat ctcattagag
      481 agcttggagg tggaaaccca gaggttcctt ctttgttaaa ccaatgtacc ttttttgtta
      541 aaactcaagg aataaatata gataaggccc atattcccga agaattaaaa aaacgtattc
      601 acgattgcaa tcctctctga tttattgttg catagtagca agtgtaggtc gggccttggc
      661 ccgacaactt ttgctaatta tctaagatat tcgagcagga aaggagaaaa aatgaaaaaa
      721 agtcccagga ttaaataaag taaaaactga tcctggatga gcatttggtc tttttttctt
      781 ggacgaagta tataaaatgc aacaaaaaga caggcaacac caatcaatat gcttaaaatt
      841 tgtactaaaa aaaatataga cataaattgt ggagaagtgg tctgggcaat gtgctcttct
      901 accggggtga ctttggcagc atatgaattc atttttttag cttaagtaaa gagattgtta
      961 taatcctcac tccatttaac caagatttca agtgcatggg aacaatcttt ctctacgatt
     1021 agaaaaatgc tggcttttat catcatcgct ttgtcaggac tcataacata gggaaggggt
     1081 aggcaaataa acgccaaatt aagtggataa ctgtaaaaac cacaaagatc catgagccag
     1141 cttcttcaaa tactaccttg atttattcac gataaactca gctggcacat gaaattttgt
     1201 tctacaccga ggtaaattca tttttttgat agataagtta ataaaatcat aatttttaaa
     1261 tgctaaaatg acatattttt aagcaattat caaaagatag tatgcccagg gctaaatttt
     1321 ttaaat
//
LOCUS       DAXLDT010000328         1308 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXLDT010000328 DAXLDT010000000
VERSION     DAXLDT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.329"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            249..758
                     /gene="lspC"
                     /locus_tag="ACF2SP_003196"
     CDS             249..758
                     /gene="lspC"
                     /locus_tag="ACF2SP_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretory system protein LspC"
                     /protein_id="HFD2360939.1"
                     /translation="MKFDIQFLFSANYAKWIIISLISLFGILIIFEWISLFYPLTYVR
                     AIPVSNTKSITGMREENLNYIFNTSLFGVYVPADLNEDNVKQSMLNVTLVGILFADKI
                     EESQVIIRSASGEEKTYNVGDKIPGGATIKRIMPGGVLVERDGTLESLSLPKNDLTFE
                     PVAKPLKEE"
     gene            764..1141
                     /locus_tag="ACF2SP_003197"
     CDS             764..1141
                     /locus_tag="ACF2SP_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD2360940.1"
                     /translation="MQRIFKLFILVIFIPMMAACIHSTLNLREGIQCFKAQDYRKAFI
                     RLKPEAEKGNPDAQYAVGYMYYYGKGVVEDRKKARFWITCAARAGQPDAIVALGILDR
                     QARRDRELFQDNNLQHFPVDNGL"
BASE COUNT          411 a          197 c          263 g          437 t
ORIGIN      
        1 tctacagggc ttacgaaaac ccctatctct ttgttaaaaa aagtttttaa ttcggtcatg
       61 tagtttattc taaacacctt cattaaaaac attttttgcc ttgaccttgg gattttttgt
      121 aagctctgat ctagtgatct tgaagtgagg gtgataaaga tctattttgt ggtaggatgc
      181 tatatatcaa tattatttaa agagaaggat gagtttgttt tgataggttt aactgggatt
      241 ggcaacgtat gaaatttgat attcaatttt tgttttcagc caattacgct aaatggataa
      301 tcattagttt aatatccctg tttggtattt tgattatttt tgaatggatt tccttatttt
      361 atccattgac ttatgttagg gctatcccgg tttcaaatac aaaatctatt actggaatga
      421 gagaagagaa tctcaattat attttcaata cgtccttatt tggagtttat gttcctgcag
      481 acttgaatga ggataatgta aaacaatcaa tgctgaatgt tactttggtc ggtattcttt
      541 ttgcggacaa gatagaagag tctcaagtga ttatacgatc tgccagtggg gaagaaaaaa
      601 cctataatgt aggagataag atacctggtg gtgcaacaat taaacgtatt atgccaggag
      661 gagtgcttgt ggaaagagat ggcactttag aaagtttaag tcttccgaaa aatgatttaa
      721 cctttgaacc tgtcgctaag cctttaaaag aggaataaat ggcatgcaac gcatctttaa
      781 gttattcatt ctagtaattt ttattcctat gatggctgcc tgtattcata gtaccttaaa
      841 tttacgagaa ggaatccaat gctttaaagc acaggactac aggaaagcat tcattcgttt
      901 aaaaccggaa gctgaaaagg gaaatccaga tgctcagtat gctgtaggtt atatgtatta
      961 ttatggtaaa ggtgtggtag aagatagaaa aaaagcccgt ttctggataa cttgtgctgc
     1021 aagagcaggt cagccagacg ccatagttgc tcttggaatt cttgatcgcc aagctagacg
     1081 ggatagagaa ctatttcaag ataataattt gcaacatttt cccgttgata atggtttata
     1141 agttttttac tttaagctaa tttccttgat tcaaatgctt tcttaaaaac ctgggtaaac
     1201 aaccaggtca ggttagtttt agctaactgt gtgcgttaat aatcaaagaa gccatgtaac
     1261 aaaaatacac aagcaaaagt atccccccag gccaggagga tgtctttt
//
LOCUS       DAXLDT010000329         1305 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXLDT010000329 DAXLDT010000000
VERSION     DAXLDT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.330"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..699)
                     /locus_tag="ACF2SP_003198"
     CDS             complement(<1..699)
                     /locus_tag="ACF2SP_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFD2360941.1"
                     /translation="MTSSKHYKESMTLLFEGSLRAIPFNIILATLLALDLVYSDVPAL
                     IIGAWFFGIVFISLVRSYFCWKIIKRGVEKNRIQATLIEFFLLTFVTGSIWGACYFIT
                     LPYVNDLHEFIIILVFGGMCAGAIASLSIYLPAYYAYVFPMLLPVIVYNYALLDVDRT
                     MLATMFLIFIAMLIISAKINHRLLNENFRLFNEKELLINELKVMSITDSLSGLYNRRY
                     FDNIFPQEFNRARRN"
     gene            996..1271
                     /locus_tag="ACF2SP_003199"
     CDS             996..1271
                     /locus_tag="ACF2SP_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360942.1"
                     /translation="MKEKHFAYKITLSSTGLVCTHSEIEENRMIDLLKEIKNDDSQAI
                     FWIFKQFDKQPKEQLGIIDCSQNRIYYHYSGEVEKIDEAIEKLSKKI"
BASE COUNT          489 a          222 c          198 g          396 t
ORIGIN      
        1 attgcgtctt gctcgattaa actcttgtgg aaatatgttg tcaaaatatc tcctgttata
       61 taaccccgac aaagaatcag tgattgacat tactttcaat tcattgatta atagttcttt
      121 ttcattgaat aatcgaaaat tttcattgag caatctatga ttaatctttg cagaaataat
      181 aagcatggca ataaatatta gaaacatggt tgctaacatc gttctatcga catctaacaa
      241 ggcataatta taaacaatga ctggtaaaag cattggaaaa acataggcat aataagctgg
      301 taaataaata gacagagaag cgatcgcgcc tgcgcacatt ccaccaaaca ctaaaataat
      361 aataaattca tgcagatcat ttacataagg cagtgtaatg aagtaacatg cgccccatat
      421 actgcccgta acaaatgtaa gaaggaaaaa ctcgatcaaa gtggcttgaa ttctattttt
      481 ttctactccc ctcttaatta ttttccagca gaaataactt cttaccaagc ttataaagac
      541 aataccgaaa aaccatgccc ctattattaa tgcaggcaca tcactataaa caaggtcaag
      601 ggcaagcaat gtagccaata taatattgaa cggaatcgct cttagagatc cctcaaatag
      661 caaggtcata ctttccttgt aatgcttgga ggaagtcatc ttatccaatg tccattaatt
      721 gatatccatt ccttttaatt atagaacacc ggctaattaa agaacacttt tgtataaacc
      781 tattcaaaat tttttatcct tattaatgta ttagagacta taatttaatc tattaagcct
      841 tttagtttgc ggggcttacg aaaaaaccaa ggtcacggca aaagatgttt ttaattatgg
      901 ggtttagata aaccaaatga ttaaattaaa aatattttta acaaagagat tgggatttct
      961 cataagtcct gatgataatt aaatggagta ctatcatgaa agaaaaacat tttgcctata
     1021 aaatcactct ttcttcaaca gggctggttt gtacccattc tgaaattgaa gaaaacagga
     1081 tgattgatct gttaaaagaa ataaaaaacg atgattccca agctattttc tggatcttta
     1141 aacaatttga taagcagcct aaagaacaac tagggatcat cgattgttcc caaaatcgta
     1201 tttattacca ttattcaggc gaagttgaaa aaattgatga ggctattgaa aaactaagta
     1261 aaaaaatata agccaaaagt gcccaaagaa atatcacatt tcatt
//
LOCUS       DAXLDT010000330         1299 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXLDT010000330 DAXLDT010000000
VERSION     DAXLDT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.331"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..232
                     /locus_tag="ACF2SP_003200"
     CDS             <1..232
                     /locus_tag="ACF2SP_003200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360943.1"
                     /translation="EDAAKDEVAAPPLNRYSVASLMTSEAAAFEVTTLWVLWECAAAS
                     AISLSGVSFMNIVSNISKLIHILPYRLINVIL"
     gene            complement(539..664)
                     /locus_tag="ACF2SP_003201"
     CDS             complement(539..664)
                     /locus_tag="ACF2SP_003201"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HFD2360944.1"
                     /translation="MAKKRCMVCGENTCEYKIKEIPFTYKGETTFIKQPGEWCET"
     gene            762..1229
                     /locus_tag="ACF2SP_003202"
     CDS             762..1229
                     /locus_tag="ACF2SP_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360945.1"
                     /translation="MPYWDDNDLVTKGAKHRHYRKGYKFTDPTHDVLSVEDSTPANLT
                     GKFGTYNYTYEPTAGYGQTVFWKLTKEFLTAQNHSVNPSITPGNRITQLSDCSKIVFD
                     NNDYLCVDVELSQSGEGANVPQYYIVENAFNTSTPVLHYYFFNKDIMPMTDAN"
BASE COUNT          431 a          216 c          217 g          435 t
ORIGIN      
        1 agaagatgcc gccaaggatg aggtggccgc gccaccttta aacagatatt cagtcgctag
       61 tcttatgaca agcgaggcag cagcatttga ggtgaccaca ttatgggtgc tctgggagtg
      121 cgcagccgcc tccgctatca gtctttcagg agtgtctttc atgaatattg tctctaatat
      181 atcaaaattg attcatatat taccatatag gttaataaat gtcatcttat aaagttaata
      241 gcacatggat tcatcgaatc gttacgctct agaaaaagaa aataatcgta ttaaaaagat
      301 tcatgtaaga gagcaactga aaggtgatat actcatagac tcaataaata caatagctta
      361 ataaaattgt ggttaattgc tgggggcggc aagaatcgaa cccgcgaaac tattgtttaa
      421 cttattgaaa aaaatttaat tttatttaaa tttatacgtt attgggcgca atttgggtat
      481 attttttatg aaaatcctag atattttagt ttctattatt tgaaaaacta caactatctc
      541 aggtctcgca ccattcgcca ggttgcttta taaatgttgt ttctccttta tacgtaaagg
      601 gaatttcctt tattttatat tcgcaagtat tttctccgca taccatgcac cgttttttag
      661 ccattttatg cctccttaaa tgaaacaagt aattcgccat tttcattaat ttgaaactta
      721 attgtacccc cataattagt gacaacctaa attagagttc attgccttat tgggatgata
      781 atgatttagt aactaaaggg gcaaaacata gacattatcg caaagggtat aaatttacag
      841 atcctacaca tgatgtactg tcagttgaag atagcacccc agcaaattta actggtaaat
      901 ttggtaccta taattatact tatgaaccaa ctgcaggata tggtcagact gtattttgga
      961 agttaacaaa agaattctta acggctcaaa atcattcagt taatccttcg ataactcctg
     1021 gtaaccgtat aacacaatta tcagattgct caaagatagt attcgataat aatgattatc
     1081 tctgtgttga tgttgaatta tcacaatctg gagaaggcgc taatgttcca caatattata
     1141 tagttgaaaa tgcttttaat acttctacac cagttctcca ttattatttt ttcaataaag
     1201 acattatgcc aatgactgat gctaactaaa tgaaaaattt ttggtaagtt ttttgattcg
     1261 tttggatatg aaaaactgaa aaattagttt ctatcaata
//
LOCUS       DAXLDT010000331         1290 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXLDT010000331 DAXLDT010000000
VERSION     DAXLDT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.332"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            283..654
                     /locus_tag="ACF2SP_003203"
     CDS             283..654
                     /locus_tag="ACF2SP_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmission trait enhancer LetE"
                     /protein_id="HFD2360946.1"
                     /translation="MNDTTSLLSHLRLRFNIEHPSYEECYIFGYECALAEVPEEDNPF
                     REGSQEAEQWLEGWWAGIYGEKPLFDLNNIEEDILLDKTTAANDQQYHHKNSFLSLVF
                     EIGGAIAASAIVGYQLLELVA"
     gene            complement(725..1114)
                     /locus_tag="ACF2SP_003204"
     CDS             complement(725..1114)
                     /locus_tag="ACF2SP_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HFD2360947.1"
                     /translation="MNKLNIAIKEAFESSGSSKEANKVYLEFIKANFIIPIESNSDDS
                     EPRVLFLEENNHVFLPVFTDMKYLDAWASEISQHIKLLKLSGVDLLKGLGEKVTVCLD
                     IGSELYKEFNPSEIERMRSMVLKLFKN"
BASE COUNT          438 a          208 c          232 g          412 t
ORIGIN      
        1 gataaaaaag ttggcctaat acttgcatct ctattaataa aatcaataag ttaatgaagt
       61 atcgtgttaa agaaccaaag gtaaaaagat ttttgtcaag ataatgtaaa tatttgattt
      121 tcgaaggcag ggggcaaatt aaagtcgtaa aattgattta agtgtgggag ttacatgcac
      181 taaaaagcag ttctgcacca attgcgttaa taattataga tttaggagta aatgagttga
      241 aaagcggaaa tatattcagg atacataaaa gaggctatca tcatgaacga tacaacatca
      301 ttattatcac atcttagatt acgatttaac attgaacacc caagctatga agaatgttat
      361 atttttgggt atgaatgtgc attggcagaa gttccagaag aggataatcc tttccgcgaa
      421 ggttctcaag aagcagaaca atggctcgaa ggatggtggg ctggcatata tggagaaaaa
      481 ccattatttg atttgaacaa tatcgaggaa gatattcttt tagataaaac cacagctgca
      541 aatgatcagc aatatcatca taaaaacagc tttctatctt tagtctttga aattggtggt
      601 gcgatagccg cttccgcaat agtaggctac caattacttg agctcgtagc atgatcatca
      661 cgttcaattg aagagcataa tgtaatgctt cttcaattga ataggagtgt tccttgtatt
      721 gagttcagtt tttaaatagt tttaaaacca tcgacctcat tcgttcaatt tcagatggat
      781 tgaattcttt ataaagctca ctgccaatat caaggcaaac tgtaactttc tcacctaaac
      841 ctttcagcag gtcaacccct gataatttaa gtaatttgat atgttgacta atttcacttg
      901 cccaagcatc gaggtatttc atatcagtga ataccggcaa aaaaacatgg ttattttctt
      961 ccaaaaataa tactctgggt tcactatcat cagaattgga ttcaatagga ataataaaat
     1021 ttgccttgat aaattcaagg taaactttat tcgcttcttt tgagcttccg gaagattcaa
     1081 aagcttcttt aatggctata tttagtttgt tcatcagggg tggatcccat gtctgtatag
     1141 tatcttgaaa atttgctttt taaacttgtt cagagtaacc aacttccata catgaaaata
     1201 tatgaagatt caactttaac cggacacggg cataccaatt tcagtcaatt atgagccctg
     1261 gtaattgata aatttgaacc tattttggtc
//
LOCUS       DAXLDT010000332         1286 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXLDT010000332 DAXLDT010000000
VERSION     DAXLDT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.333"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            403..1218
                     /locus_tag="ACF2SP_003205"
     CDS             403..1218
                     /locus_tag="ACF2SP_003205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD2360948.1"
                     /translation="MKAALITGATHGIGLALTRNLLSKGWTVFGVGRDQDSLDRTSSE
                     FATFRPICADLTNNDDLKKVADVVNNTKIPLNILVQNAGMKTPPRDLEKHTCEAIDEV
                     IGLNLSAPMKLTALLIPCMPSGSRILYVTSRAATLKLMQSSTYCATKAALDEVAAIVR
                     QELREKNIAVGSVIPGEVNTSIQKTLRETTGFHLHRMFQQAYESGQLIRPEICADFLK
                     WLLCDLSFEEFNNGEIHTIYDDAHHKYWLANPEDLPAFPFEDKPQNTLLMSKL"
BASE COUNT          405 a          233 c          259 g          389 t
ORIGIN      
        1 aaattatttc atagtattaa tctgcaagaa catttaaagc acgtttagct atttttatac
       61 aaaagcataa ttgcaagaat aaagcccagt tattagaatg tgttgtccac tctcaattat
      121 tttatccaat ttttttgaaa gagagcggca aatcaacagg cgacccctgc cgatcggaga
      181 gttaaagcac agtagaatta cggtattgat actctcctta tcatttatta gaatgcgtga
      241 aaattatcct tggcatatgt ggctctaatc catgcctgag tcgcctgggt gtctctgaca
      301 caataaagat gtgggtaatg atatggttaa ttagatgctt actcttggca ttgtgccagc
      361 cttattgaag gatctggact attctatact aggggaatta aaatgaaagc agccttaata
      421 acaggtgcaa cacatggtat tggtttggct ctaacacgca atttactgag taaagggtgg
      481 actgtttttg gcgtaggccg agatcaagac agtctggata gaacaagcag cgaatttgct
      541 acattcagac ctatctgcgc tgatttaacc aataacgatg atcttaaaaa agtggctgat
      601 gttgttaata atactaaaat ccccttgaac attttagtac aaaacgctgg catgaagacc
      661 ccaccgcgag atttagaaaa gcatacctgc gaagcgattg atgaagtcat aggtcttaat
      721 ttatcagcgc ctatgaaact tacagcattg ttgatccctt gcatgccatc aggatcgcgt
      781 attttatatg tcacttcgcg tgcagctacc ttgaagttaa tgcaaagctc aacttattgt
      841 gcaactaaag cggctttaga tgaggttgca gctattgtca gacaagaatt gcgtgaaaaa
      901 aatatcgctg ttggatcagt aattcctggt gaagtaaaca cgagtatcca gaaaactctg
      961 cgagaaacta ccggttttca tttacacaga atgtttcaac aagcgtatga atcggggcaa
     1021 ttgattcgtc cggaaatttg tgcggatttt ttaaagtggt tgttgtgtga tttatccttt
     1081 gaagaattta ataacggtga gatacatacc atttatgacg acgcacatca taagtattgg
     1141 ttggctaatc cagaagattt acccgcattt ccatttgagg ataaaccaca aaatacgcta
     1201 cttatgtcta agctttaact ttaggctatt aaacctaata aaaatcaaat ggtttgatca
     1261 ttcaaatttt gggcaaatgc caacct
//
LOCUS       DAXLDT010000333         1283 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXLDT010000333 DAXLDT010000000
VERSION     DAXLDT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.334"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            155..1114
                     /locus_tag="ACF2SP_003206"
     CDS             155..1114
                     /locus_tag="ACF2SP_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HFD2360949.1"
                     /translation="MSFKGFKVVMLILLSTQSYASKLASSSVCDSTIENPCIVQDSKT
                     QFSPVIRYREVASIADVYGGNITGINKFHLSGSEQPSEKGWEAIAESISRKMGPETKK
                     VIVLDLRQESHGYLNGRAITLVSVYNWINLGKSNSQSTLDQENWLTGLRSRKIVNGVL
                     TVPQYVAKQYSQGKSMVVSTVKNEEYYVYKKGFDYYRIFISDHRAPLDSEVDALVALI
                     KNNPEDTWYHVHCRGGKGRTTTVFAMFDMLKNADKVSFEEIIARQASIPPFYNLMVTN
                     REIPELTPYYEQRLQFLIHFYEFARQSLMGYSGTWSEWKKLNI"
BASE COUNT          405 a          211 c          266 g          401 t
ORIGIN      
        1 tttatttgga cagttgaata aagtatagaa ttatttggca gtaacgtatt aatgattgtg
       61 attttatttc tgaaatggta atgcgtcttg aataataaaa atcttttaca tagaatgctg
      121 cggttcatct ttagggattt aaaaaggatt tattatgagc tttaaaggat ttaaagtggt
      181 aatgctgata ttattatcaa ctcaaagtta tgcctctaaa ttggcatcct ccagtgtttg
      241 tgattcaact attgaaaatc catgtattgt ccaggatagt aaaacccaat tttcacctgt
      301 cattagatat agagaggttg catcaattgc ggatgtttac ggcggcaata tcacagggat
      361 taataaattt catttgtcag gaagcgagca accaagcgag aagggttggg aggctattgc
      421 tgagtccatt tccagaaaaa tggggcctga aacaaaaaaa gtcatagtgc tcgatcttcg
      481 ccaggagagt catgggtatc taaatggcag agcgattact ttagttagcg tctataactg
      541 gataaatttg ggcaagtcga atagtcagag tactttggac caggaaaact ggttaaccgg
      601 cttaagatcc agaaaaattg ttaacggtgt tttaactgtg ccccaatatg tagccaaaca
      661 atattctcaa ggcaaatcga tggtagtgag cactgtaaaa aatgaagaat attatgtata
      721 taaaaaagga tttgattatt atcgcatctt catatccgat caccgggctc ctttagattc
      781 tgaagtggat gcgcttgtcg cactgataaa aaataatccg gaagacacat ggtatcatgt
      841 ccactgccgg ggcgggaaag gtcgtacgac gaccgttttt gccatgtttg atatgttaaa
      901 gaatgcagac aaggttagct ttgaagaaat catcgcacgc caagcttcca ttccgccttt
      961 ttataatttg atggtgacca atcgtgaaat ccctgagtta actccttatt atgagcaaag
     1021 actgcaattt ttgattcatt tttatgaatt tgctcgccaa tccctcatgg gatattcagg
     1081 tacctggtca gaatggaaaa agctcaatat ctagcatcta gcgccatttg caagaagatc
     1141 atattaaaaa gccttcattt gaaatgaagt gaatttcatg aaggcttggc actcaatcgt
     1201 tttcggcaaa taccatatta atcttaaatt aagtgttggt tttcttagga tttatctgtc
     1261 ccaagataag aaattcttga ttt
//
LOCUS       DAXLDT010000334         1265 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXLDT010000334 DAXLDT010000000
VERSION     DAXLDT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.335"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..938
                     /locus_tag="ACF2SP_003207"
     CDS             <1..938
                     /locus_tag="ACF2SP_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFD2360950.1"
                     /translation="AYAKIPDSSASRAQNINDNKGAKNANNFLKKITPEFAYSYIDFN
                     FDSTAGLNFNRYNGHSNLYSIGADHFSPVPSIIAGLYVLNIDTEVSSQFQLFPNTPIP
                     SSQTIHNNTLFAHMRKIFTPKFDIDIAGGYGQNRINSQTLLNRGITDIAFSKHTNDNW
                     LASINAVYRKRGKKISLRTYVGMLYSQINSGSYILAFQSNQRGQIIAPLTTKTTYIME
                     GAEISYKLKPALTPFVNGGLIQIADFTNSRPILTQQINGSLPQLNMDKNGYKLGAGVI
                     FNYKQMTLRLEEKYYSAGNIFESYQTTAGLEYRFN"
BASE COUNT          421 a          249 c          203 g          392 t
ORIGIN      
        1 cagcctatgc taaaattcct gatagctcag catcaagagc acaaaatata aatgacaata
       61 agggcgcaaa aaacgctaat aactttttaa agaaaatcac gcctgaattt gcttacagct
      121 atatagattt taactttgat tcgacagctg gtcttaattt caatcgatac aatgggcatt
      181 ccaatcttta ttccataggt gctgatcatt tttctccagt accgagcatc attgcagggc
      241 tttacgttct aaacatagat actgaagtga gttctcaatt tcaattgttt cccaatactc
      301 caattccttc cagtcaaacg attcacaaca acacattatt cgcacatatg cgtaaaatat
      361 ttaccccaaa atttgatata gatatcgctg gaggatatgg tcaaaacaga ataaattcac
      421 aaactttgtt aaatcgcggc atcactgata tcgctttttc aaaacatact aatgacaact
      481 ggcttgccag catcaatgca gtttatagaa aacgaggaaa aaaaatatct ctaagaacct
      541 atgtgggaat gttatacagc caaattaatt cgggtagtta tatattagcc ttccagtcca
      601 accaacgcgg gcaaatcata gctcctttaa ctacaaaaac aacctatatt atggaagggg
      661 ctgaaatcag ttacaaactc aagcctgcat taaccccttt tgttaacggt ggtttgattc
      721 aaatagcgga cttcaccaat agccgaccta tacttacgca acaaattaat ggctctctgc
      781 ctcagctcaa tatggataag aatggctaca aacttggtgc tggagttatt tttaattata
      841 aacaaatgac tttaaggctt gaagaaaaat attacagcgc aggaaacatt tttgaaagtt
      901 atcaaacgac tgccggactg gagtaccgat ttaattagtt gattgtatag taatttattt
      961 attgttcttc atatcggagg cacgaaaatt aattttgcaa taacatggaa caaatatact
     1021 tgtgatctgc caggcttaaa aacattattt ctgcccttct ctcgaagact atatactgat
     1081 taataaagag gacgctcttt catcttgtgt ttaagagtag caaaaggata attttcgtga
     1141 ataaattaac tctttatttc cctcaattcc tcaatcacat aaagtggact gagtttttct
     1201 tggggctgac agtaaccctt ttagtgttac taaccttgtt ctttaatgtt cccctactca
     1261 attcg
//
LOCUS       DAXLDT010000335         1238 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXLDT010000335 DAXLDT010000000
VERSION     DAXLDT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.337"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            123..>1238
                     /locus_tag="ACF2SP_003208"
     CDS             123..>1238
                     /locus_tag="ACF2SP_003208"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFD2360951.1"
                     /translation="MNNRVSRIEQVIQSYVADKQFMGAILMAQHEQVVLDKGYGFANL
                     EWQISNTPKTKFRIASLTKQFTAAAILLLEEQGKLKTSDFINKFMPDAPSTWNKVTLF
                     HLLNHTSGIPNYTGFPDFAKFTTSTKTPEQQIELFRHKPLNFQPGSNFEYNNSGYVLL
                     GYLIEKISGQSYEDFIMTNIFNPLSMNDSGYDSHSKIIINRASGYMVSSNGICNADYL
                     DMSIPYSAGSLYSTTRDLLLWEQGLFGGTILSPESLNKMINPFKNEYGLGVRIHSLEE
                     HKSITHAGGTSGFNTKLIYSPDDKLTVIVLANLNALGYVAQDLALKIVALARGKTVKL
                     PSERKEIVIPTEQLVKYTGKYTIKPYIAPYGLTSEKHL"
BASE COUNT          431 a          227 c          216 g          364 t
ORIGIN      
        1 aattacaaat tagatattca ttttgacaaa taaactcaga ctaatgaaat taaatgagct
       61 tatagcagaa aatacaaaaa atcatgatca aatttcttat ataatgttta aagggaaacg
      121 ccatgaataa tcgggtgtcg cgtatagagc aggttataca gtcgtatgtg gcagataagc
      181 aattcatggg cgcaattctt atggctcaac atgagcaagt agtactggat aaaggatatg
      241 gctttgctaa tcttgaatgg caaatctcca acacaccgaa gacaaaattt cgcattgcct
      301 cgcttacaaa acaatttaca gctgctgcga tattgttact tgaagagcag gggaaactta
      361 aaacttccga tttcattaat aaattcatgc ctgatgctcc atcaacatgg aacaaagtga
      421 ctctcttcca tttattgaac cacacatctg gtattcctaa ttacactggt tttcctgatt
      481 tcgctaaatt tacaacaagt acaaaaacac cggaacagca gatagaactc tttcgtcata
      541 aaccattgaa ttttcaacct ggctcaaact ttgaatacaa caattcagga tatgtacttc
      601 ttggctactt gattgaaaaa ataagcggtc aaagttatga agattttatt atgactaata
      661 tattcaaccc attgagcatg aatgactcgg gatacgactc ccattcaaag ataattataa
      721 atcgagcttc tggatatatg gttagctcca atggaatttg caatgcagat tatttagata
      781 tgagtatccc ctattctgct ggttctcttt actctaccac gcgagatttg ctcttgtggg
      841 aacaaggttt atttggagga acaattttat caccagagtc cttaaacaaa atgataaatc
      901 cgtttaaaaa tgaatatggc ttaggtgtga ggatacactc tctggaagaa cataaatcaa
      961 taacacatgc tggcggcacc agtggcttca atacaaagtt aatctattca cctgatgata
     1021 aactgactgt cattgtatta gctaatctaa atgcattggg ctacgtagct caagatcttg
     1081 ctttaaagat tgtagctttg gctaggggta aaacagttaa attgccatct gagagaaaag
     1141 aaatagtaat acccacagaa caacttgtta aatatactgg gaaatacacc ataaaaccct
     1201 atattgctcc atatggatta acgtcagaaa agcacctt
//
LOCUS       DAXLDT010000336         1218 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXLDT010000336 DAXLDT010000000
VERSION     DAXLDT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.338"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            145..1164
                     /locus_tag="ACF2SP_003209"
     CDS             145..1164
                     /locus_tag="ACF2SP_003209"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD2360952.1"
                     /translation="MIERAFSLLFIQFSIMQIQNTEINIGVIIVNYNSAHCLSKAITA
                     LEKQSYKPYQIILFDNSSIENLPEIVHTTKLPIKIVVSNINLGFAAGNNKAIEYLDPE
                     VNWIALLNPDAYPHKDWLLEMVSAIKTHPQYAFMGSKLICENEPYLLDGTGDVYHISG
                     KAWRLNHRKPLDKAPKDMQELFAPCAAAALYRRDAFNAVKGFDEQYFCYYEDVDLAFR
                     LWLAGYKGCYIPTAIAEHTGSAITKKHSDFYTYYGHRNLVWTYFKNMPLLLLIATLPL
                     HLLLNIATLLLFIKRKQTFVIIKSKIDAIKGLPTLLQQRRHIQKSRVLSNFQLLKILK
                     KGLPW"
BASE COUNT          428 a          209 c          195 g          386 t
ORIGIN      
        1 aaatatttta ttttttcatc tgcgcaaaaa tgaaaataaa ttcacaatat atagcaaaaa
       61 gactgcatat ccaataaaaa caaagcatta atttaaatat cattaaagat taaaaaatga
      121 tattcttggc ataaactaga gtaaatgata gaacgcgctt tttctttgct cttcatccaa
      181 ttttcaatta tgcaaattca aaatactgaa attaatatag gcgttataat tgttaattat
      241 aattcagcac attgcctctc taaggcaatt actgctttag agaagcaatc atacaaaccg
      301 tatcaaataa tactctttga taattcgagt attgaaaatt tacccgagat cgtccacaca
      361 actaaattac caattaaaat tgttgtatcc aatattaacc tgggatttgc agctgggaac
      421 aataaggcca ttgaatatct agatccagaa gtcaattgga tagcattgct taatcctgac
      481 gcctacccgc acaaggactg gttactagaa atggttagtg caattaaaac tcatccgcaa
      541 tatgctttca tggggtcaaa actaatttgt gaaaatgagc cttatctatt agatgggaca
      601 ggcgatgtgt accatattag cggaaaagca tggagattaa accacagaaa accattagac
      661 aaagccccca aagatatgca agaactgttt gctccctgcg ctgcagctgc cttgtataga
      721 cgtgatgcat tcaatgctgt aaaagggttc gatgagcaat atttttgtta ttatgaagat
      781 gttgatttgg cttttcgatt atggctagca ggttataaag gatgttatat tcctacagct
      841 atcgctgagc atacaggctc cgccataaca aagaaacata gtgattttta tacttattat
      901 ggccatagaa atctagtttg gacctatttc aagaatatgc cattgttatt gcttattgca
      961 actttaccct tacaccttct tttaaatatt gctactcttc ttttattcat taaacgaaag
     1021 caaacttttg taattataaa atctaaaatt gatgcgatta aaggtttgcc aacactacta
     1081 caacaaagaa ggcacattca aaaatcgaga gttctttcta attttcaatt actaaaaatc
     1141 ttgaaaaaag gcttgccatg gtagctaatt tagtaacagt tgttatagtc aattataatt
     1201 cgggcgaaat gcttggta
//
LOCUS       DAXLDT010000337         1206 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXLDT010000337 DAXLDT010000000
VERSION     DAXLDT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.339"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..441
                     /locus_tag="ACF2SP_003210"
     CDS             <1..441
                     /locus_tag="ACF2SP_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360953.1"
                     /translation="QKMSEDLLKLLDEANTAGIEIPNEDKMRNYLDCALEAINIQSKP
                     KAIKDYDQAINYFITGVKETLATKPPKPEEVHWFKKFLRFITGNEKLLQNADEKRYDQ
                     QIKVLSGISDLNKKLNSLSQEKNISSEHEHTHDHGHGNQMTFSN"
     gene            complement(694..957)
                     /locus_tag="ACF2SP_003211"
     CDS             complement(694..957)
                     /locus_tag="ACF2SP_003211"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD2360954.1"
                     /translation="MMMSFAGEGWGEGMSRMVIDDEMWSRLEELLPKPKGRHDKNERL
                     SMEAICTMLCAGATWCDLPPYHGNWKSVYNRYNNWSKKGYITT"
     gene            complement(1129..>1206)
                     /locus_tag="ACF2SP_003212"
     CDS             complement(1129..>1206)
                     /locus_tag="ACF2SP_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFD2360955.1"
                     /translation="GSRKKKLALIEKLNPYWEDLYEKLL"
BASE COUNT          389 a          248 c          213 g          356 t
ORIGIN      
        1 caaaaaatgt cagaagatct cttgaaatta ctcgatgaag cgaatacggc aggcattgag
       61 atacctaatg aagataaaat gcgaaactat ttagattgtg cattagaagc gattaacatt
      121 caatcaaaac caaaggctat aaaagattat gatcaagcta ttaattattt tattactgga
      181 gtaaaagaga cattggcaac caagcccccc aaacctgagg aagtccactg gttcaaaaag
      241 tttctaaggt ttatcacagg caatgaaaag ctattgcaaa atgctgatga gaaacgttat
      301 gaccaacaaa tcaaagtcct ttctggtatt agtgatttaa ataagaaact gaattcttta
      361 tcacaagaaa aaaacatatc ctctgaacat gaacacactc atgatcatgg acatggaaat
      421 caaatgacat tctctaatta gagaaactac tttgattact agggaagtga tttcgtccca
      481 cctcaataac cttgctctga aaggttattg agatctggac ttacgactta tgggtttaag
      541 gtcttgcgct tttgaaatca tgacgttttt cagcaaacgt cttctgccct cgaagttatc
      601 atgtctgcat tacttttatg ctaaaatata ctcaaaagca taaagccagt gaagtacatt
      661 ctggctgttt tatcataccc tgtagcaatc cttctaagtg gtgatataac cttttttcga
      721 ccagttatta taacgattat aaacactttt ccagttgcca tggtacggtg gcaagtcaca
      781 ccatgtcgca ccagcacaaa gcatcgtaca tattgcctcc atagataacc gttcattttt
      841 atcatgtcgt ccttttggct ttgggagtaa ctcttctaaa cgagaccaca tttcatcatc
      901 aatgaccatc cgtgacattc cctctcccca accctctccc gcgaaagaca tcatcatacc
      961 aaagttccta tcgcgggagg ggggggcagg tcgaagtgga attgaaaata tgtgctcatt
     1021 tcaagattaa attttgtcta aaaaacctct gtaaggtacg atttttagga ccgtcgttgc
     1081 gaggagcgta gcgacgaagc aatccagtga ccttaaaatt gctaatcttc acaacagttt
     1141 ttcgtaaaga tcttcccaat aagggtttag cttttcaatt aaagccaatt tcttttttct
     1201 tgaccc
//
LOCUS       DAXLDT010000338         1203 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXLDT010000338 DAXLDT010000000
VERSION     DAXLDT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.340"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(236..1057)
                     /locus_tag="ACF2SP_003213"
     CDS             complement(236..1057)
                     /locus_tag="ACF2SP_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360956.1"
                     /translation="MISLSFLFANAHELAALLELPYVQRLVYLMGIRPYMDRQSCIVG
                     IKRRISYQSLRETLYVGPIAGVKTGSPSLQQIKRAVKSLERAGLIEIQSTEKHLIVKC
                     IYAELDNPAQKKAVPKPNLYPDTNPTQEDALESGESCNFGQKPNIANNTEADPPQKSE
                     DYNYIYMCSKFEKFWKSYPHPVDREAAFYQFRELNPDDKLFEKIMTGLHGQIESYNLQ
                     VMLSEWVPKWKYPSNWLAKKCWQTDPYVFEQSLPVVHDLENQQIGIQEDSNLDHT"
BASE COUNT          349 a          240 c          220 g          394 t
ORIGIN      
        1 agatacttct acccagtcat atacttactc ccgattaacg caatatgtcc aatcccgata
       61 ggcgggatcg gacaaaatag cgaaatacct tgattttact aggggatatt aaagtgattt
      121 ttctgcgtca ctgctggaaa attgcagtga cgcagaaaac aatagacgat agaaaagtta
      181 ttattctttc tcaaagtgca aagcattact tatcgagctt ccctcatctc tgaaattaag
      241 tatgatctag attgctgtcc tcttgaatgc ctatctgttg attttccaag tcatgtacaa
      301 ctggtaatga ttgctcaaac acatacggat ctgtttgcca acattttttg gctagccagt
      361 tagatggata tttccatttc ggcacccatt cacttaacat tacctgaaga ttataacttt
      421 caatttgccc atgcagtccc gtcataatct tttcaaataa cttatcatca ggattaagtt
      481 ctctgaattg ataaaaagcc gcttccctat caacgggatg tggataactt ttccaaaatt
      541 tttcaaattt gctacacata taaatataat tataatcttc tgacttctga ggtgggtcgg
      601 cctcagtgtt attggctatg ttgggttttt gtccaaaatt acatgattcc cctgattcta
      661 aagcatcttc ctgtgtcggg tttgtgtcgg gataaaggtt cggcttcggt acggccttct
      721 tttgagcggg gttatctaat tcggcgtaaa tacattttac tatcaagtgc ttttcagtgg
      781 actggatttc aataagacct gcacgttcaa gagatttaac agcccgcttt atttgctgaa
      841 ggcttggact gcccgtttta acgcctgcaa tcggcccaac atagagggtt tcacgtaacg
      901 attgataact gatcctgcgc tttatgccta caatgcaact ttgtctgtcc atgtatggac
      961 ggatgcccat caggtaaact aatctttgta cataaggcaa ttcaagtaac gccgctaatt
     1021 catgggcatt ggcgaataaa aaactcaagc taatcatcct taaaatcatg cggtattctc
     1081 attttagatg tgtcatccgg atagtcaata tgattagaaa tggtgttact aaatgatatt
     1141 gtttgaacca aaaaataatt tgagtttata aatattccaa ataatctagc catactagaa
     1201 tat
//
LOCUS       DAXLDT010000339         1196 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXLDT010000339 DAXLDT010000000
VERSION     DAXLDT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.341"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1196
                     /locus_tag="ACF2SP_003214"
     CDS             <1..>1196
                     /locus_tag="ACF2SP_003214"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HFD2360957.1"
                     /translation="AAFLNPPNPYVRPLRTSDTLNINYDSAVALPEVYTNKNLIINKL
                     LLNIYEQDLLFLANGNACEAKKDFANFYNPYYTAIGKALLPKLEQLAFNFLKDEIEII
                     GNWTKPKLLDYLINTILEQEKETSELCEAIVGSNNPIRAAKMLLIQMAPDFLSEASAM
                     ARALPGSFGIEQSELMKVFIDEYGYGVHETKHSTLFEATMLSANLNPTIHYYYNDYLA
                     TSLMLVNYFHCICKNKSDWFKYIGTLYYTEASIPHFNKQISKTLKNILPNINTKYFDE
                     HVHIDKHHRRMVLEKIIATSIDKYGEEIIEDILFGFEAFKLLQNLADTDLAKQIQFSD
                     QLDESYFPSNYTLLAESLTFNETELEITSAHLHADDELFQVTRGELIFYPNSLSPIIL
                     KANHRI"
BASE COUNT          421 a          197 c          195 g          383 t
ORIGIN      
        1 tgcagcattt ctaaatcctc ctaatcctta cgtacgacca ttaagaacca gtgatacgtt
       61 aaatattaat tatgattcag cagtggcttt acctgaagta tatactaata aaaatctaat
      121 cataaataag ctcttgctta atatttatga acaagatctt ttgtttctgg ccaatggcaa
      181 tgcatgtgaa gccaagaaag acttcgctaa tttctataat ccatattata cagctattgg
      241 caaagcattg ttgccaaaac ttgaacagct agcattcaat tttttaaagg atgaaattga
      301 aataataggt aattggacca aaccaaaatt attagattat ctgataaata caattctgga
      361 gcaagaaaaa gaaactagtg agctttgcga ggctatagtt ggttctaata atcctatacg
      421 ggcagccaag atgttactca ttcaaatggc gcctgatttt cttagtgaag cttcggcaat
      481 ggctagggcg ttaccaggct cctttggtat tgaacaatca gaattaatga aggtatttat
      541 agatgagtat ggttatgggg ttcatgaaac aaaacattca actttgtttg aagctacgat
      601 gctttcagcc aatttaaatc ccactataca ttattactat aatgattatc tagccacgtc
      661 actaatgctc gttaattact ttcattgtat atgcaaaaat aaatctgatt ggttcaaata
      721 tattggtacg ttatattaca ctgaagcaag catccctcat tttaacaaac aaattagtaa
      781 aaccttaaaa aatattttac ctaatataaa tacaaaatat tttgatgagc acgtacatat
      841 agacaaacat catcgacgta tggttcttga aaagataata gccaccagta tcgataaata
      901 tggggaggaa atcatcgagg atattttatt tggatttgag gcattcaaat tacttcaaaa
      961 cctagcagat actgatttag caaagcaaat tcaattcagt gatcaactag atgaaagcta
     1021 ttttcctagt aattacacac ttcttgcgga gagtttaaca tttaatgaaa ctgaacttga
     1081 aattacttct gcacatttac atgcagatga tgaattgttt caggtcacta ggggtgagtt
     1141 aattttctat cccaatagcc taagtcctat tatattaaag gctaatcata gaattg
//
LOCUS       DAXLDT010000340         1188 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXLDT010000340 DAXLDT010000000
VERSION     DAXLDT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.342"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            19..1017
                     /gene="aph(9)-Ia"
                     /locus_tag="ACF2SP_003215"
     CDS             19..1017
                     /gene="aph(9)-Ia"
                     /locus_tag="ACF2SP_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HFD2360958.1"
                     /translation="MLRNNIPDQHLIALLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQVDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREIVRSFYSQ
                     LGFDERDDQITTDFKAFFNQNIDSIHQLVNSSGELSKKIQPDFEKYVLCHSDVHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGSGIGNVWNKPHEINYFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHDLLSRDQNAQSRLESFKHFKSMFTPNDVVEIAFATGLI"
BASE COUNT          402 a          200 c          202 g          384 t
ORIGIN      
        1 aaatagtaat taactcttat gctaagaaat aacattccag atcaacacct cattgcgctt
       61 ttgaaagtct attatggaat tgatattcat acagtacaac tcatcgtggg tggtgctgat
      121 atgaatgcct ttggatataa agcagattca gaatccaatt cttatttcgt aaagctaaaa
      181 tacggccatc atgatgaaat taatttatca atcatccgtc tcttacatga ctctggagta
      241 aaggaaattg tttttcctat caacacactg gaagcaaact tatttcagca agtggatcat
      301 gttaaaataa ttgtgtatcc atttattgat gcgcccaatg gcttcaccaa aaatttaaca
      361 gaaaaacagt ggaaacagct tgggaaaata ttaagacaaa tccatggaac ctctgttccc
      421 acctcgattc aacagcgatt aagaaaagaa acttactcac ctaaatggcg tgaaatcgtt
      481 aggtcttttt acagtcaact tggatttgat gagcgtgatg atcaaataac cactgacttc
      541 aaagcttttt ttaatcaaaa tatcgattca atccatcaat tagttaattc ttcaggagaa
      601 ctgtctaaaa aaattcaacc tgatttcgaa aaatatgtac tttgtcattc agacgtacat
      661 gcaggtaatg tgttggtcgt tgatgaggaa tctatttaca ttattgattg ggatgagccc
      721 atgttcgcac caaaagaacg tgatttgatg ttcattggca gtggtattgg taatgtatgg
      781 aataaacctc atgaaatcaa ttatttttat gaaggttatg gtaaaacaag catcgataaa
      841 accattttgt cttattacag acatgagcga attgttgaag atatagccgt gtatggacat
      901 gacttgcttt cacgtgatca aaatgctcaa tccagacttg aaagttttaa acattttaaa
      961 tcgatgttta ctccaaatga tgtagttgaa atagcttttg ctacaggttt gatttgattt
     1021 ggataaacac agaagaaaaa agatgaaccg tttaatctca accctcctct ttttaatttc
     1081 gtataaactt tgaattgcat taaatcacct gaaatttttt atataacgca tcccaacttt
     1141 agtaagaaga cgacatgact gtttgtgtta tcgcaatgat aagctctt
//
LOCUS       DAXLDT010000341         1176 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXLDT010000341 DAXLDT010000000
VERSION     DAXLDT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.343"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..670
                     /locus_tag="ACF2SP_003216"
     CDS             <1..670
                     /locus_tag="ACF2SP_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein SidG"
                     /protein_id="HFD2360959.1"
                     /translation="NSTGLKVGLTLMALPAMIVLAPLAAIQKGVEIIGETIAKPFKFI
                     ANLFKQKPESTDEITVSVGSKKVAEKEGSYSNTALAGLVNSKIKSKIDENTITVEFQK
                     SPQKMIEAFESQLKENPGKVVVLSEKAHNAVLKFVSNSDDEALKQKFYDCCNQSLARS
                     QKFAPKTRDEIDELVEEVTSTDKTELTTGPTQEPSMSSTIEEEESIDSEHQIESGTES
                     TMRI"
     gene            832..>1176
                     /locus_tag="ACF2SP_003217"
     CDS             832..>1176
                     /locus_tag="ACF2SP_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFD2360960.1"
                     /translation="MPKLILLGDSTLDNFNWVPNKATVNDHLNRQLPKMEILNFAVDG
                     FTTDSILYGEYKDCAVNSSQHIHKYFEPLKVLKNEKEVEHIVLSVLGNDFRIQLSKLI
                     FMAPEERPKAIEQ"
BASE COUNT          444 a          181 c          211 g          340 t
ORIGIN      
        1 caattctact ggattaaaag ttggattaac tctcatggca ctacctgcaa tgatagtatt
       61 agctccttta gcagcaatac aaaaaggcgt ggaaatcatt ggtgaaacga tagccaagcc
      121 atttaaattt atagcaaatt tatttaaaca aaagcctgaa agcactgatg agattactgt
      181 ttctgtaggc agtaaaaaag tggctgaaaa agaaggtagt tattcaaata ccgctttagc
      241 tggattggtt aatagtaaaa taaaatcaaa aattgatgaa aacacaatta ctgttgaatt
      301 tcaaaaatca cctcaaaaaa tgatagaagc atttgaatca caattaaagg aaaatcctgg
      361 aaaagttgtg gtattaagtg aaaaagcaca taatgctgtc ttgaaatttg tatcaaacag
      421 tgatgatgag gcactaaaac agaaatttta tgattgttgt aaccaatcac tagcacgtag
      481 tcaaaaattc gcacccaaaa ccagggatga aattgatgaa ttggtggaag aagttacatc
      541 tactgataaa acagaattaa ctaccggccc cacacaagaa ccatcgatga gttctactat
      601 tgaagaggaa gaaagcatcg attcggaaca tcaaatagaa agtggaactg aaagtacgat
      661 gcgtatataa attaaaacga tttcatcatt gttcttttca tgtgatttaa ctacttggtg
      721 gtttcacagt caagagagtt ccaaaaaata ttattttgtt aaaatacctg gaagccataa
      781 tttctccaaa aagaatccaa cacatttatt tatagtggga ggtaataagt tatgccaaaa
      841 ttaattttat taggagactc cactcttgat aattttaatt gggtgccaaa taaagccact
      901 gtaaatgatc acttaaatcg tcaactaccc aaaatggaaa ttcttaattt tgctgttgat
      961 ggatttacaa ctgactcaat cttatatgga gaatataaag actgcgccgt caattcctct
     1021 cagcatatcc acaaatattt tgaaccactt aaggtgttaa aaaatgaaaa ggaggttgaa
     1081 catatagttt tgagtgttct gggtaatgat tttcgcattc agttaagtaa gctaattttt
     1141 atggctccag aagagaggcc aaaagctatt gagcaa
//
LOCUS       DAXLDT010000342         1173 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXLDT010000342 DAXLDT010000000
VERSION     DAXLDT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.344"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            69..848
                     /locus_tag="ACF2SP_003218"
     CDS             69..848
                     /locus_tag="ACF2SP_003218"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD2360961.1"
                     /translation="MLSVIIITKNEEANIRRCLESVHFADEIIVLDSGSTDNTLAIAR
                     EYTDKVFSTDWQGYGIQKDRALQKAQGDWVLNLDADESVSSELRQEILQAISSDTADA
                     FRIPIQMIFYNQVLKYSGSPKRHIRLFKRENASYSKDIVHEKVLIPVNARVGKLKKPI
                     WHHSFSDVHHVLYKLNKYSSYSAKIRIESNEKVGLVKTFFSALWMFVRCLFLQKGFLD
                     GRAGFLFAVFGAQGAFYRGVKQIYKDRDINKLPSVNHIKEE"
     gene            851..>1173
                     /locus_tag="ACF2SP_003219"
     CDS             851..>1173
                     /locus_tag="ACF2SP_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen biosynthesis protein"
                     /protein_id="HFD2360962.1"
                     /translation="MRLTIMDTKGILLAPFFFISFMFFIPISPTIKSICFICSLIAVL
                     ITPQYRKHIVYAYNTLWGRAAFCLFLFVVIASLWSPAPYSMQWMVIGKYCKLIYLPIL
                     ATGFI"
BASE COUNT          368 a          178 c          222 g          405 t
ORIGIN      
        1 tcctttttac cttgttacaa tccggcttaa ttatcgatat aatatttatt ttgaattgat
       61 ataaagtcat gttatccgta ataattatca caaaaaatga agaagccaac ataaggcggt
      121 gcttggaatc cgttcatttt gcagatgaaa taattgtatt ggactctggc agcacagata
      181 atacattagc tatagccagg gagtatacgg ataaagtatt ttcaacagat tggcaaggct
      241 atggtatcca aaaggatagg gccttacaga aggctcaagg ggattgggtt ttaaatcttg
      301 atgctgatga atcagtaagc tcggaattac gacaggaaat actacaggcc atttcatcgg
      361 atacagcaga tgcatttcgt attcctatac aaatgatttt ttataatcaa gttttaaaat
      421 attcaggaag tcctaaaagg catatacgat tatttaaaag agagaatgct tcttatagca
      481 aagacatcgt tcatgaaaaa gtattgattc ctgttaatgc gagagtggga aaactaaaaa
      541 aaccgatttg gcatcattct tttagcgatg tacatcatgt actatacaaa cttaacaaat
      601 attcttctta tagtgcgaaa atccgtatag aatccaatga aaaagttgga ttagtaaaaa
      661 ctttttttag cgccctatgg atgtttgtca gatgcctttt tttacagaag ggatttttag
      721 acggcagggc aggttttttg tttgcagtat ttggggcgca aggcgctttt tatcgcgggg
      781 tcaaacaaat atataaagac agggatatca ataaattacc cagtgtaaat catataaaag
      841 aggaataatc gtgcgattaa ccataatgga taccaagggt attttattag ctcctttttt
      901 ctttatttca tttatgtttt ttattccgat cagtcccacg ataaagtcta tttgtttcat
      961 ttgcagttta attgccgtat tgattactcc acaatacaga aaacatattg tttatgctta
     1021 taatactttg tggggacgcg ctgcattttg tttgtttctc tttgtcgtta ttgcaagttt
     1081 atggagtcct gcgccttatt caatgcaatg gatggttatt ggtaaatatt gtaagttaat
     1141 ttatcttccc attttagcta ctggttttat taa
//
LOCUS       DAXLDT010000343         1170 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXLDT010000343 DAXLDT010000000
VERSION     DAXLDT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.345"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            656..>1170
                     /locus_tag="ACF2SP_003220"
     CDS             656..>1170
                     /locus_tag="ACF2SP_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD2360963.1"
                     /translation="MTIIDARPTCFYHPIKVIFLDDNRAFLDALDLEFGEKFNILTLT
                     SSDMAFHLIDNDSQDVTQSIFKLMNDVNLDTTNDRVIGFDVSKMLNLIYDKARFEHAP
                     LLVVDYQMPDINGIEFCKKIKEKKIFKIMLTAEADKDTAISAFNNGLIDKFILKTSEN
                     LYPEITLAVDDL"
BASE COUNT          426 a          147 c          199 g          398 t
ORIGIN      
        1 ggcaaagctt gttgtttttt aaatattaaa gtaatcaacc tatactaaat gtgtgacgaa
       61 ttaattatct acaaatatta attaataggg ttgcattcta attttatgat aatcttaatt
      121 atctttagga tgatgttttt tgtttttgga ctgttgaatt ttttattaaa ttcgatcctg
      181 tctctgtttc aacaagcgct tttaatttta cgagacttct actaggaatt atcggtttca
      241 taaattgtcc ccgggggata attttagtaa cttattgatt ataaacaata aaaataaaca
      301 atattctgta attcacatgg gggttatgga atggtgtaga tttatgaggc ggcaaatttt
      361 aaagcaaatt atggtgatta caaattaaca aacgtttgag agaaaaaaaa ttaacaggaa
      421 aagtattcaa acttaatcgc aaaatataaa caactacaac ttaagtgaca aaatataaaa
      481 ttgaggagga aaaaatttga ataatcattt attatacaaa gcgacatcca tgttagttta
      541 attttcctta acagcaatga taatggaaca atacagtata aattaggagt ctgaaatatt
      601 ggcgatttat catgggtgtg atgcaattaa agctgtaacg tagaggaatt actatatgac
      661 tattattgat gcaagaccta cctgttttta tcatccgata aaagtcattt ttttagatga
      721 taaccgtgca tttttggatg cgttagattt agaatttggc gaaaaattta atatactgac
      781 acttacaagc tcagatatgg cttttcatct gattgataat gatagtcagg atgttaccca
      841 atcaattttc aaattgatga atgatgttaa tctagatact actaatgatc gggttattgg
      901 ctttgatgtt agtaaaatgt taaacctgat ttatgataaa gcaaggtttg agcatgcccc
      961 actgttagta gtggattatc aaatgcccga tattaatgga attgaatttt gcaaaaaaat
     1021 taaagagaaa aaaatcttta aaattatgct aacggcagaa gcagataaag atacagcgat
     1081 cagcgcgttc aataacggtt taattgataa atttatactc aagacaagcg aaaatctata
     1141 tccagaaatt acattagctg tcgatgacct
//
LOCUS       DAXLDT010000344         1157 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXLDT010000344 DAXLDT010000000
VERSION     DAXLDT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.346"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1127)
                     /locus_tag="ACF2SP_003221"
     CDS             complement(<1..1127)
                     /locus_tag="ACF2SP_003221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360964.1"
                     /translation="MALDGYEGKRLPLNAEDVLAQGMFDFMVQEVLAKRMGITLAHER
                     DPVGDERVRFNQHFQSLFLPWVLGEAPESVCNVIADAKTSESALSFVLSRFEAIGFMP
                     DDIMMQRVRGSCSEAGLEAIPCNFTPLLATLHAGMARHASALFLRPNGVPATSSQKVA
                     QQTIQWLRGPDFSPEALNAQLERGTPFQNVYLVLRFLDALQQYKERKHLLHVCDMPVE
                     DKAAWVESLAFFGACLQDPEPSLERLLADTPRLEERIRAFERGFSVWRSNSYQDFITN
                     YFAQWFGNEEISARGQLFTRLCELYARETAQSENPAIRVSDAMLKEWTGQIGEDGLLP
                     VEPYQINRILLHALCYPQHTWTPLFRENLSILLRFIRSGFN"
BASE COUNT          295 a          321 c          293 g          248 t
ORIGIN      
        1 tcgttgaatc cactgcggat aaagcgcaga agaatgctga ggttttcgcg aaatagaggc
       61 gtccatgtgt gttgaggata gcaaagcgca tggagcagga tgcggtttat ctgataaggt
      121 tcgacaggaa gcaaaccgtc ttcgccaatt tgcccagtcc actctttaag catggcatca
      181 ctgacccgga tggcagggtt ttcactctgt gccgtttcgc gagcatacag ctcacagagc
      241 cttgtgaaca gctgaccgcg cgcacttatc tcctcattcc caaaccattg ggcaaaataa
      301 ttcgtgataa aatcctgata gctgttgctc cgccagacag aaaagccacg ctcaaacgca
      361 cgaatacgct cctcaagccg tggcgtatcc gcaagcagcc tctcaagtga tggctctggg
      421 tcttgcaaac atgccccgaa aaaggcaagg gattcaaccc atgccgcttt atcctctacc
      481 ggcatgtcgc agacatgcaa taggtgtttg cgttctttgt actgctgaag cgcatcgagg
      541 aagcgcagaa ccaggtacac gttctggaaa ggagtgcctc gctccaactg cgcattcaaa
      601 gcttccggac taaaatccgg accccgcagc cactgaatgg tctgctgagc aaccttctgc
      661 gagcttgttg ctggcacccc attcggacgt aagaagagtg ctgaggcatg gcgagccatc
      721 cccgcatgca acgtggcaag aagtggcgtg aagttgcagg gtatggcctc gagtccggct
      781 tcgctgcaag aaccacggac tcgttgcatc atgatgtcat caggcatgaa cccaatggcc
      841 tcaaagcgcg acagtacgaa actcaaggcg gattcggagg tctttgcgtc cgcaatgaca
      901 ttgcagacgg attccggtgc ctcaccaagt acccatggca aaaatagaga ctgaaaatgt
      961 tggttgaacc tcacacgctc atcacctaca gggtctctct catgcgcaag cgtgatgccc
     1021 attctcttgg caagcacctc ttgcaccata aaatcaaaca tgccctgcgc gagaacgtct
     1081 tccgcattca agggtaagcg cttgccttca taaccatcca gtgccatgag ccaagcatgg
     1141 cgaatgccag tcgagca
//
LOCUS       DAXLDT010000345         1151 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXLDT010000345 DAXLDT010000000
VERSION     DAXLDT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.347"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(85..1149)
                     /locus_tag="ACF2SP_003222"
     CDS             complement(85..1149)
                     /locus_tag="ACF2SP_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD2360965.1"
                     /translation="MILLFSILISTVVYAQSKIKICLTGRVVENLKSYGQSFLNAAYL
                     AKEENDPANKVEIKSYFYNNRPLEPMHIYNQMVNDSCSAIIGFEYLSDLLLAVKEQKN
                     DTIPIFTSYASTTNSDQLPKNIFIFMPSYNYQADKMMSYLQDRYKNINNVLLITEINR
                     DEMLKYKEVYSSTLKKEHIQYSTFDFLENDSDIEGKLKKFLNERKYQYVFLLSGAIAS
                     AKIANIMNNHCTVFIGTENFGSSVSQTFFMRLNDKVIRSHFIRNLDFIQPYDALTRFE
                     NIYVQKYHEKPTVLAVYTYDSMKIILKALDRTGIVNTNSILNTNYTGITGAYLKNGQF
                     YRSTNYVILSVNSNGYAYEK"
BASE COUNT          384 a          163 c          184 g          420 t
ORIGIN      
        1 atgagtataa atccaaaaat aataaattgg attagagcag aatatgccat tttcttgata
       61 aaaattgctt tgattgaaga gcttttattt ttcataagca tatccattgc tattaactga
      121 taaaataacg taattggtcg atcgataaaa ttgaccgttt tttaaataag ctccagtaat
      181 tcctgtataa ttagtattta atatgctatt tgtattgacg ataccggttc tatccaatgc
      241 ttttaaaatg atcttcattg aatcataagt gtatactgcc aatacagtgg gtttttcatg
      301 gtatttttga acataaatat tttcaaatct ggttaaagca tcatagggct gtataaaatc
      361 caaattacgg ataaaatgag aacgaatgac tttatcattt agtcgcataa aaaatgtttg
      421 actgactgaa gaaccaaagt tttctgtgcc aataaaaaca gtacaatggt tattcataat
      481 attggcaatt tttgctgatg ctattgcacc tgacaataaa aaaacgtatt gatattttcg
      541 ctcattcaaa aatttcttca gttttccctc tatatcgctg tcattttcta gaaaatcgaa
      601 tgtagaatat tgtatgtgtt ctttttttag agtgctggag tacacttcct tatactttag
      661 catctcatca cggttaattt ccgttataag taacacgttg tttatatttt tataacgatc
      721 ttgtaagtag ctcatcattt tatcagcttg gtaattatag ctgggcataa agataaaaat
      781 attcttgggt aattgatcag agttagtggt gctagcgtaa gaagtaaaaa taggtatggt
      841 gtcatttttc tgttctttta cagctaataa caagtcggaa agatattcaa acccaataat
      901 tgcggagcag gagtcattta ccatttgatt ataaatatgc attggctcca atggtctgtt
      961 attataaaaa taactcttga tttcaacttt attagcagga tcattttctt ctttggcaag
     1021 atatgccgca tttaaaaaag actggccata tgattttaaa ttttcaacaa ctcttccggt
     1081 taaacatatt ttaattttag attgtgcgta aacaacagtg gatatcagta tagaaaataa
     1141 taatatgatg g
//
LOCUS       DAXLDT010000346         1144 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXLDT010000346 DAXLDT010000000
VERSION     DAXLDT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.348"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(147..1046)
                     /locus_tag="ACF2SP_003223"
     CDS             complement(147..1046)
                     /locus_tag="ACF2SP_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HFD2360966.1"
                     /translation="MWNLSSRVHALNHFPKQGIECYVKRDDELGCGISGTKIRKYSSL
                     IPFLKIKGIRHLIIIAGAQSNNLLAALQVARECQLKVTAFLVKPKHLKIQGNFKLSLL
                     FLHENEIIWVNREEWYRVNEFAEQYLEGLRETAYILSEGASVKESMKGAMSLANDIKE
                     NEKILGFAFDHIFVDAGTGFSAIALIKGFHELQHKGFIHVLLLADSEEVFKKKLMHWA
                     GVNPDNYACFYPTTAKSFGSVNQTIKQEIKRLAYEEGILADPIYSAKLFYEARRYIEK
                     YELKGKVLIVHSGGILTMPGFAF"
BASE COUNT          383 a          236 c          168 g          357 t
ORIGIN      
        1 agtatagtag ataatcgaga cagagatatt caggaaaaaa tcgtatcatg tactaaaaaa
       61 tactgaatat ccttgatgaa cttaattttg cagtcaggaa aacgtgataa ttcagaaatt
      121 acgtctttgc caagaagcaa gcctattcaa aaagcaaagc caggcatggt taatatacca
      181 ccagaatgta caatcaatac cttccctttt aattcgtatt tttctatgta ccttctggcc
      241 tcataaaata attttgcaga gtagatcgga tcagccaaaa tgccttcttc ataagccaat
      301 cgtttaattt cttgtttaat tgtttgattt acagatccaa acgatttcgc agtggttggg
      361 taaaaacaag cgtaattatc agggtttact cctgcccaat gcataagttt cttcttaaaa
      421 acctcctcac tatctgctaa caataacaca tgaataaatc ctttatgctg taattcatga
      481 aaacctttaa ttagcgcgat tgctgaaaaa cctgtacctg catcgacaaa gatatgatca
      541 aaagcaaatc ccaaaatctt ttcattttcc ttgatatcat tggctagaga cattgccccc
      601 ttcatggact ccttaacact ggcaccttca gataaaatat aagcagtttc tctgagcccc
      661 tcaagatact gttcagcgaa ttcattgact cgataccatt cttctcgatt tacccagata
      721 atttcattct catgaagaaa aagaaggctt aatttaaaat taccctggat ttttaaatgt
      781 ttgggcttta ctagaaaagc ggtcactttc aattgacatt ctctggccac ctgcagagca
      841 gccaataaat tattagattg agcacccgca ataataatga gatgtcgaat tcccttgatt
      901 tttagaaacg ggattaaact gctgtatttc cttatctttg taccacttat gccgcaacct
      961 aattcatcat ctctcttaac ataacactct ataccttgct tagggaaatg attaagggca
     1021 tgcacccgac tggacaaatt ccacatcaaa tacctttata aataatttaa tggattcgaa
     1081 taaaattcaa actatttgga ctggaaattt ctttattaaa taaataaccc gactcagaaa
     1141 actc
//
LOCUS       DAXLDT010000347         1100 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXLDT010000347 DAXLDT010000000
VERSION     DAXLDT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.349"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..632
                     /locus_tag="ACF2SP_003224"
     CDS             <1..632
                     /locus_tag="ACF2SP_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD2360967.1"
                     /translation="VITGISRGIGLEIARIFLDKDWTVIGTSTSGYSPIKHKNLRTYK
                     LDLAISEHIDAFAKNLPKIDVLINNAAVLLEEWNEEKINMSLLKKTFAINLFGTVELT
                     EKCIPKFNDGAQIINISSGWGAFSSNNSPFQPHYKMSKASLNMYTKLLAERLPQITVS
                     SFDPGWVKTNMGTQNAKKLPSETALEIYELISMKKKSGYFWLNGKPRDW"
     gene            690..>1100
                     /locus_tag="ACF2SP_003225"
     CDS             690..>1100
                     /locus_tag="ACF2SP_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360968.1"
                     /translation="MSFDFIKSIWIAALIGLTPTVSYSESNIQYQETLQSHCFETWMK
                     KIDPSIDKETYKQFGDKYCRCISTKNLDNKTGVQKAIRNCIPQTILHDAMDELEEEVT
                     LSEVTTQIIEQYCLNRWSLIYPNQSDEDKKTIQAY"
BASE COUNT          413 a          189 c          197 g          301 t
ORIGIN      
        1 tcgtcataac aggaatcagt agaggcatag gcctggaaat tgctagaatt ttccttgaca
       61 aagattggac ggttattggt acttccacca gcggatattc tcctataaaa cataaaaacc
      121 tgcgcacata caagttagac ctagcgattt cggagcacat cgatgcattt gcaaaaaatt
      181 tacctaaaat tgatgtactg attaataatg ccgccgtgtt gcttgaagag tggaatgaag
      241 aaaaaattaa tatgtcctta ttgaaaaaaa cctttgcaat taacctgttt ggtacagttg
      301 agttaactga aaaatgtatc cccaagttca atgatggtgc acaaatcatt aatatctctt
      361 cagggtgggg agcatttagt tccaataatt ccccctttca accacattat aaaatgtcaa
      421 aagccagttt gaatatgtat actaaattgc ttgcggaaag acttccccaa ataactgtat
      481 ccagttttga tccaggttgg gttaaaacaa acatgggaac acaaaatgca aaaaaacttc
      541 ccagtgaaac ggcactggaa atctatgagc tgattagtat gaagaagaaa agtggctatt
      601 tttggcttaa cggaaaacca agggattggt agccagttaa atttcagtta ttctcaacta
      661 taaaataaag aatcaaagga gtgacaaacg tgagtttcga ttttataaaa tcaatatgga
      721 ttgcagctct tataggttta acaccaaccg tgtcttattc agagtccaat attcagtatc
      781 aagaaacact tcaatcacat tgctttgaaa cctggatgaa aaaaatagat ccatcaattg
      841 acaaagaaac atataaacaa tttggagata aatattgtcg atgcatttca actaaaaatc
      901 tcgataataa aacaggggtt caaaaagcaa ttagaaattg cataccacaa acaattttac
      961 atgatgccat ggatgaactg gaggaagagg tgactttatc agaagtaacg acacaaataa
     1021 tcgaacaata ttgtcttaat agatggagcc ttatttatcc caaccaaagt gatgaagata
     1081 aaaaaacaat ccaggcctat
//
LOCUS       DAXLDT010000348         1085 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXLDT010000348 DAXLDT010000000
VERSION     DAXLDT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.350"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(447..>1085)
                     /locus_tag="ACF2SP_003226"
     CDS             complement(447..>1085)
                     /locus_tag="ACF2SP_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360969.1"
                     /translation="MFGVLVAYTKFFAINPYDKKSMSIYQSVKKEFEKDLNHIESSYS
                     ELLKDKDFNSALQVEMKELEIRLKYVLFNWFFLFKKDKQDLSKQICQLKVDVLRLKTK
                     FMISHLLLNHDKYNLEHYKFVVKHLHNHLEDLNDKIGLIKGIDVDIYNDLFSEMYEWF
                     EQIYSLLNNRYDEFKKEFSIGGKWDDWSYDNSSSEDDWDDNEPLVFLNNTIP"
BASE COUNT          360 a          198 c          157 g          370 t
ORIGIN      
        1 gataatagca cgcttgggac atgcttatgg tttgattaaa aaagaagcga aaaaacaatt
       61 taaatttcgc atttattcgg ttgcaacaac attcattatc atgggaattt tagcgatttt
      121 taatgtaagt aaagcgacga tactatatat cgttatataa taatttagat gattttagca
      181 ggacttacga aaaactccaa gatcaaggca aaaaatgttt ttaatgaggg agtttagata
      241 aactaaatga ccgaattaaa aacatttttt aacatagaga ttggggtttt tcgtaaatcc
      301 tgtttaggtt tttggcgatt tgccccctat aatctgcgga tgaaacaatg tagatactca
      361 tcaaatacat attgacttac gcttgctaca gaagccttac cctactgaat aattctcatt
      421 gagccgcatc acgatatatt aattcattaa ggaatcgtat tgtttaagaa aaccaggggc
      481 tcattatcgt cccaatcatc ctcgctacta ctattgtcat agctccaatc atcccattta
      541 ccgcctatag aaaactcctt tttaaactca tcatatcgat tgtttaatag agaatatatt
      601 tgctcaaacc attcatacat ctcactgaac agatcgttgt aaatatcaac gtcaatacct
      661 ttaatcaggc ctatcttatc attgagatct tccaggtgat tatgtaaatg cttaaccaca
      721 aatttgtagt gctcaaggtt gtatttatca tgattcaaca gaagatggct tatcataaat
      781 tttgttttta atctcaatac atctactttt agttgacaaa tttgcttgct caaatcttgc
      841 ttatcctttt tgaataagaa gaaccaatta aaaaggacat actttaacct aatctccagt
      901 tctttcattt ctacttgcaa cgccgaattg aaatctttat ctttgagtag ttcactataa
      961 gaggactcga tatggtttaa atccttctcg aattctttct taaccgattg gtaaatactc
     1021 atacttttct tatcatacgg attgatagca aaaaactttg tgtaagccac taaaacccca
     1081 aacat
//
LOCUS       DAXLDT010000349         1068 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXLDT010000349 DAXLDT010000000
VERSION     DAXLDT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.351"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..937)
                     /locus_tag="ACF2SP_003227"
     CDS             complement(<1..937)
                     /locus_tag="ACF2SP_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360970.1"
                     /translation="MKITKTVLMFLIIFSSEVFAESTKPVTVFNYPVEAIYLINNPKD
                     GDISWGHDYEEACKKMGDRFFWGIVPSKSVDPACYSLPTYNMIIGADTWFCANGSISY
                     FNKPHNFCNDFPSCPDQSWTLATDKSICTRENRPCIPDPDSVSEEQLLAAVAYGESHW
                     SNDYEEMAGIASATLRRMKAYGYKSVSELISKDPNFAYATALKTKNERYYNLMCNINS
                     NGVELAYKAARNALSNGADYSNGGCFWDGVDVKTNGASTYRYKYGFKFSNPKHNVLDV
                     TEPKPFHKKGRNGKYFSHTYVSTAGHNKTIFWKLSK"
BASE COUNT          328 a          208 c          167 g          365 t
ORIGIN      
        1 ctttacttaa tttccaaaaa attgttttat tatgtcccgc agtagataca taagtatggc
       61 taaaatactt accattcctc ccttttttat ggaagggttt aggctcagta acatcaagta
      121 cattgtgttt tgggtttgaa aatttaaatc cgtatttata tctataggta gatgctccat
      181 ttgtttttac atctacccca tcccaaaaac atccaccatt tgaatagtct gccccattcg
      241 acaaagcgtt cctagcagct ttataggcta attctactcc attgctgtta atattacaca
      301 tcaaattata gtaccgttca ttttttgtct taagggctgt ggcataagca aagttgggat
      361 cttttgaaat gagttcactg actgatttat atccatatgc tttcattctt cgcaaagtag
      421 cactcgcaat tcctgccatt tcttcataat cattgctcca atgtgattcg ccataagcaa
      481 cagcagctaa taattgctct tcagaaacac tatcagggtc aggaatacaa ggtctatttt
      541 cgcgagtaca aatggattta tctgttgcta atgtccaact ttgatctggg cacgaaggaa
      601 aatcattgca aaaattatga ggcttattga aatagctaat tgacccattt gcacagaacc
      661 atgtatctgc tccaattatc atgttatatg ttggtaacga atagcaggca ggatcgacac
      721 ttttggaagg aacaataccc caaaaaaatc tatctcccat ttttttgcag gcttcctcgt
      781 aatcatggcc ccaagaaata tctccatctt tagggttatt gatcagataa atggcttcaa
      841 caggataatt aaaaaccgtt actggcttag ttgattcagc aaatacctcg ctagagaaga
      901 taattaaaaa catcaaaact gtttttgtaa ttttcaacca atctactaac attaagtgtc
      961 attaaacaaa agtgcttata ttttaacata atatttttct ttagatgcac tctgtttata
     1021 aatgttgata actctgcatc ttcctgcgat aggtttatcg attagttt
//
LOCUS       DAXLDT010000350         1064 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXLDT010000350 DAXLDT010000000
VERSION     DAXLDT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.352"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(100..606)
                     /locus_tag="ACF2SP_003228"
     CDS             complement(100..606)
                     /locus_tag="ACF2SP_003228"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2360971.1"
                     /translation="MSILVETPRLLIKTPILDDLENWYVLHSDEDVMEYMGGVQSKSV
                     IQEWLQSDILHYHKHGFTMGSVFKKNNNEFIGRAGLVYLDHNDNQPDIEIGYVLHKKY
                     WGQGYGVELVKALIDWGFAHLAVDKLVVVTRSENTQSKRLLEKCGLHYVRIIAFRNTD
                     FLLYEVYK"
BASE COUNT          350 a          193 c          152 g          369 t
ORIGIN      
        1 taacgggtta ttctatgaat aagaacaact attttgtgct atcaatcgcc gacttttaat
       61 ctcttcaagt attttgtctc ttgggctaat ctgaaagcat tatttataca cttcatataa
      121 tagaaaatct gtattacgaa atgcaatgat tcttacataa tgcaagccac atttttctaa
      181 taaacgcttt gactgtgtat tttcagagcg agtaacaacg actaatttat caacagctaa
      241 atgagcaaat ccccaatcaa ttaatgcctt cactaattca accccatatc cctgacccca
      301 atatttttta tgtaaaacat atcctatttc gatatcaggt tgattatcat tgtgatcaag
      361 ataaacaagt ccagcacggc ctatgaattc attattgttt ttcttgaaaa cagaccccat
      421 agtgaaacca tgtttatggt aatgcaaaat atcactttga agccattcct gtattactga
      481 cttgctttgg actcccccca tatactccat aacgtcttca tcagaatgca gcacatacca
      541 attctcgaga tcatctaaga tgggtgtttt aattaaaagt cgtggggttt ctactaatat
      601 gctcatgtat tgtgactttg ctctatatat tattttaacg taaacgaatg atagctttaa
      661 gatttaaaat taatttttgc tcaaagagtt attccatttt caaatagcaa aagtaactgg
      721 aacctcgtag tatcgcactt ttccccagac caaaggaaat tatgatatga atatgcgcca
      781 gcttgagact attttctgcg aaatagatga cttctgcgat cattaatgcc aattttacta
      841 tccgagtatt attaaataat gctcggataa tttgtttaat ttaactaatg agttctatct
      901 tttgattcaa gttattgata tctagcaaat tctagcctga ttgcatgcag cttatcagtg
      961 attttgtaca atgctgaaaa tccttaaaaa caaggaatag tttaaattaa ggttaagtct
     1021 taaaagcaat agaaaaaact ttaacattaa tttttgagca tctc
//
LOCUS       DAXLDT010000351         1044 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXLDT010000351 DAXLDT010000000
VERSION     DAXLDT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.353"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..417
                     /locus_tag="ACF2SP_003229"
     CDS             <1..417
                     /locus_tag="ACF2SP_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HFD2360972.1"
                     /translation="FEVFKKGLREAVKFIVDNKSIPVIMFDNPPLINLSKTCGLTRVS
                     FLDCSNNLREVKKIQNATRNIILNLKNEFPQLILIDPTKIICDKSKCYTSINGTPLYF
                     DEGANSHLNYDGSTLIGKLYLKKFGNPFQTKNSIYS"
     gene            complement(543..737)
                     /gene="csrA"
                     /locus_tag="ACF2SP_003230"
     CDS             complement(543..737)
                     /gene="csrA"
                     /locus_tag="ACF2SP_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFD2360973.1"
                     /translation="MLILTRRIGETLIIGDDVNITVLGVKGNQVRLGINAPKDVSVHR
                     EEIYLRIQQEKESDDSEQAV"
BASE COUNT          376 a          179 c          153 g          336 t
ORIGIN      
        1 tttgaagtat tcaagaaagg attacgtgaa gcagtaaaat ttattgttga taacaaaagt
       61 atcccagtaa ttatgtttga taatccaccc ttgataaacc tgagtaaaac ttgcggattg
      121 acaagggtat cctttctgga ctgctccaat aacttacgtg aggtaaaaaa aattcaaaat
      181 gctaccagaa atattatttt gaacttaaag aatgagttcc cacagcttat attaattgat
      241 ccgacaaaaa taatatgcga caaatcaaaa tgctatacct caattaacgg gacaccatta
      301 tatttcgatg aaggtgcaaa ttctcatttg aactatgatg gttcgacact tattggaaaa
      361 ctatatctca aaaaatttgg taatccattt caaacaaaaa actcaatata tagttagtat
      421 taattttgat ttgctgatga gtgtataaga agctttatta aagaggaaat tacatttagt
      481 tacatagtgc gtcccgagta tttttaagta ctcggaaccg gatgagctgc gtggaaatcg
      541 atttatactg cttgttccga atcatcagat tctttttctt gttgtatgcg taaataaatt
      601 tcttcacgat gaacagaaac atcttttgga gcattaattc ctaagcgaac ttgattgcct
      661 tttacaccta aaacagtaat attgacatca tcacctatga ttaatgtttc acctatacgc
      721 cgagtcaaaa tcaacatgac taaaatctcc ctaacatagc agctacctac ttctccatag
      781 aacgcttcca tggtgttctt ggcaactaca atatgaacaa aatttaagca aaatttattg
      841 cttggatcac attgtacact ctgattatta acggaatatt aagtcataga taaatttgcg
      901 tttaattttt gcagaaatga gttgaaaaat taataaattc cttgtcataa attcatcata
      961 aaactgttca acattcccta atatcaaaaa ctcccaatta taaataaact ggactcaaaa
     1021 ggaaaaacaa gtttttttcc tgca
//
LOCUS       DAXLDT010000352         1002 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXLDT010000352 DAXLDT010000000
VERSION     DAXLDT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.354"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            363..>1002
                     /locus_tag="ACF2SP_003231"
     CDS             363..>1002
                     /locus_tag="ACF2SP_003231"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD2360974.1"
                     /translation="MTRYFMLLGVLLIWGVTIVTHAEPQWLKLPVTPSLPKAEHSGYA
                     SINGVQIWYATYGHGQPVILLHGGLGNSSYWGNQVPILAQHYRVIVMDSRGHGRSTRN
                     EQSFSYDLMTSDVLGLLDFLKIKKVAIIGWSDGAIIGLLIAIHHPSRLTKLFAYAANS
                     NPSGIKADIFKSSVFKNYVARTKNEYKALSSTPKDYKILFVQLSKMSASQPHF"
BASE COUNT          343 a          160 c          202 g          297 t
ORIGIN      
        1 tatggaaggg cattggtata gtttaaacga agcagaaaaa tgcaaatcag ttggcgtgac
       61 ggttttaaat atttttgata atgccgaaga gctgatcgaa gtaccaagtt tgactatggg
      121 gttataatat tggcacaact gataacgtaa caggagaaaa tccgaatttt ggtattagtc
      181 caaaatcgag cccatcgagt cagactcaat tgataataga aataaaagcc aaactgcaat
      241 ataaaaacaa gatggaactg gatatcaagc ttgctgatta actacgtaaa tgaaaaggga
      301 atcacaatca tatcatttaa taaaccaatt ggaattaaat aaaaaaatta aaggtcagtc
      361 atatgacacg ttatttcatg ttattggggg tgctgttaat ttggggagtt actatagtaa
      421 ctcatgctga acctcaatgg ttaaaattac cagtaacacc aagtttgccc aaagcagaac
      481 atagcggtta tgcttcaatt aatggtgtcc aaatttggta tgccacctat ggtcatggac
      541 aaccagtaat cttgcttcat ggcggtcttg gtaattcaag ctactgggga aatcaggtac
      601 cgatactggc tcagcattat agagtcattg tgatggatag cagggggcat ggaagaagca
      661 caaggaatga gcaatctttt agttatgact taatgacctc agacgtactc ggtctattgg
      721 atttcttaaa aattaagaaa gttgcaatta ttggttggag tgatggtgca attatagggc
      781 tacttattgc gatacatcat ccaagtcggt tgacgaaact ttttgcttat gctgctaatt
      841 ctaatccttc tggtatcaaa gcagatattt ttaaaagttc agtatttaaa aattatgttg
      901 ctcgaacaaa gaatgagtat aaagcattat catctactcc aaaagattat aaaattctct
      961 tcgttcaact cagtaaaatg tcagcctctc agccacattt ta
//
LOCUS       DAXLDT010000353         1001 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXLDT010000353 DAXLDT010000000
VERSION     DAXLDT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.355"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..696
                     /locus_tag="ACF2SP_003232"
     CDS             <1..696
                     /locus_tag="ACF2SP_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HFD2360975.1"
                     /translation="WGYNGFTQVGGWQHWHDVDHEWDWGFKLEGSYHFNTGNDINVNW
                     YHFDNDSDHWADFANWHNYNNKWDAVNAELGQFVDFSANKKMRFHGGVQYARIEADVN
                     RYFNNFAFNGFNSKFNGFGPRTGLDMNYVFGNGFGVYAKGAAAILVGTSDFYDGINFI
                     TGSKNAIVPELEAKLGADYTYAMAQGDLTLDVGYMWFNYFNAMHNTGVFNGFETDFAA
                     SGPYIGLKYVGNV"
BASE COUNT          273 a          172 c          238 g          318 t
ORIGIN      
        1 tggggctaca atggtttcac ccaagttggt ggctggcagc attggcatga tgttgaccat
       61 gagtgggatt ggggcttcaa attagaaggt tcttatcact tcaatactgg taatgacatc
      121 aatgtgaact ggtatcattt tgataatgac agtgatcact gggctgattt tgctaactgg
      181 cacaactaca acaacaagtg ggatgctgtt aatgctgaat taggtcaatt cgtagatttc
      241 agcgctaaca agaaaatgcg tttccacggc ggtgttcaat acgctcgcat tgaagctgat
      301 gtgaaccgtt atttcaataa ctttgccttt aacgggttca actctaagtt caatggcttt
      361 ggtcctcgca ctggtttaga catgaactat gtatttggca atggctttgg tgtttatgct
      421 aaaggcgctg ctgctattct ggttggtacc agcgatttct acgatggaat caacttcatt
      481 actggttcta aaaatgctat cgttcctgag ttggaagcta agcttggtgc tgattacact
      541 tacgcaatgg ctcaaggcga tttgacttta gacgttggtt acatgtggtt taactacttc
      601 aacgctatgc acaatactgg cgtatttaat ggatttgaaa ctgatttcgc agcttctggt
      661 ccttacattg gcttgaagta tgttggtaat gtgtaatttg ttaagttgat aagaaatttc
      721 agcaatactg ttgactttat agaagtccgg ctggataatt tatccaggta cgctctcgat
      781 tagagcgaag aatgcgtact gttgctgata tgctaaatta gtaacgaacg gaaattaata
      841 ataaaaaaag tggagataag catgttaaat ttgaaaaaaa cagcggtagc tgttcttgct
      901 ttaggaagca gtgcagtgtt cgctggtact atgggaccag tttgcacccc aggtaatgta
      961 actgttcctt gcgaaagaac tgcatgggat attggtatca c
//
LOCUS       DAXLDT010000354          997 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXLDT010000354 DAXLDT010000000
VERSION     DAXLDT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.356"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..977
                     /locus_tag="ACF2SP_003233"
     rRNA            <1..977
                     /locus_tag="ACF2SP_003233"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          212 c          312 g          215 t
ORIGIN      
        1 gcgtaaaggg tgcgtaggtg gttgattaag ttatctgtga aattcctggg cttaacctgg
       61 gacggtcaga taatactggt tgactcgagt atgggagagg gtagtggaat ttccggtgta
      121 gcggtgaaat gcgtagagat cggaaggaac accagtggcg aaggcggcta cctggcctaa
      181 tactgacact gaggcacgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca
      241 cgctgtaaac gatgtcaact agctgttggt tatatgaaaa taattagtgg cgcagcaaac
      301 gcgataagtt gaccgcctgg ggagtacggt cgcaagatta aaactcaaag gaattgacgg
      361 gggcccgcac aagcggtgga gcatgtggtt taattcgatg caacgcgaag aaccttacct
      421 acccttgaca tacagtgaat tttgcagaga tgcattagtg ccttcgggaa cactgataca
      481 ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgtaacgag
      541 cgcaaccctt atccttagtt gccagcatgt gatggtgggg actctaagga gactgccggt
      601 gacaaaccgg aggaaggcgg ggatgacgtc aagtcatcat ggcccttacg ggtagggcta
      661 cacacgtgct acaatggccg atacagaggg cggcgaaggg gcgacctgga gcaaatcctt
      721 aaaagtcggt cgtagtccgg attggagtct gcaactcgac tccatgaagt cggaatcgct
      781 agtaatcgcg aatcagcatg tcgcggtgaa tacgttcccg ggccttgtac acaccgcccg
      841 tcacaccatg ggagtgggtt gcaccagaag tagatagtct aaccttcggg gggacgttta
      901 ccacggtgtg gttcatgact ggggtgaagt cgtaacaagg tagccgtagg ggaacctgcg
      961 gctggatcac ctccttacat agaaaggcac agaagga
//
LOCUS       DAXLDT010000355          994 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXLDT010000355 DAXLDT010000000
VERSION     DAXLDT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.357"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..908)
                     /locus_tag="ACF2SP_003234"
     CDS             complement(<1..908)
                     /locus_tag="ACF2SP_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2360976.1"
                     /translation="MNKFPQSFSEANERIGNAIHHVCLNASDAKFIKSNIQESYQSWA
                     NVLYDRVFLNWGLCDKKIYRELSALNFDFSKVGFFPHVYGQSLLFYLIRPLIKIHFFN
                     KRLLDVGCGNGVGLKISSELLKTRYALGLDLVNKLVTNSNNSFYIEDKINYMQADAEN
                     MAIANESFDIVTNLESSHLYPQIEHFFSEVERVLAPNGFFCYADVNFDVKRQAERFET
                     FVKTRKNLRIIEKHNITKMIQASIYQRLIVNEKGFYQLAQYLLGPDKIHYTSELANVA
                     SSMGLMFLPWWRIRFKSPALQALGKFA"
BASE COUNT          332 a          191 c          150 g          321 t
ORIGIN      
        1 gcaaattttc ccagcgcttg caatgccgga ctcttaaagc gaatcctcca ccagggaaga
       61 aacatgagtc ccattgaact ggccacatta gccagctccg aggtatagtg aatcttatca
      121 ggccccaaaa ggtattgagc caattggtaa aatccttttt cattgacaat taaacgctga
      181 tagattgatg cctggatcat tttagtaata ttatgctttt caataatacg taaatttttt
      241 cttgtcttca caaaagtttc gaatctctcc gcttgccgct tcacatcaaa attaacatcc
      301 gcataacaaa aaaatccgtt aggtgctaaa accctttcta cttcagaaaa aaaatgctcg
      361 atttgaggat aaagatgtga gctttcaagg ttagtgacaa tatcaaaact ttcgtttgct
      421 atagccatat tttctgcatc ggcctgcata taattaattt tatcttctat atagaagcta
      481 ttgttggaat tagtgaccaa tttgttgacc aaatcgaggc ctaacgcgta tcttgttttt
      541 aataattcag aacttatttt caaacctacg ccattaccac aacccacatc aagtaatctt
      601 ttattaaaga aatggatttt tattaatggc cttatcaaat aaaaaagaag tgattgccca
      661 taaacatgag ggaaaaaacc tactttagaa aaatcaaaat tcaatgccga taattcccga
      721 tatatttttt tatcacataa tccccagttc aaaaaaactc tgtcataaag cacattggcc
      781 cagctctggt agctctcttg aatattggat ttaatgaatt ttgcatccga tgcatttaaa
      841 cagacatgat gtatcgcatt acctattcgc tcattcgctt cactaaagct ttgcgggaat
      901 ttattcattt ttgaattttt aaaattaata atataagtat agacagcaag aagttgacat
      961 tgaattagtg agccaaattt tagataaaat tcta
//
LOCUS       DAXLDT010000356          987 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXLDT010000356 DAXLDT010000000
VERSION     DAXLDT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.358"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>987)
                     /locus_tag="ACF2SP_003235"
     CDS             complement(<1..>987)
                     /locus_tag="ACF2SP_003235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFD2360977.1"
                     /translation="LTTSQATYLNFLRGFSAIIVLAGHTLSGIPGVISFGKQLPFQSL
                     AVNAFFWLSGFLITYHCITKKPYTFAEYMIDRFCRIYVIYIPVLILSVFLLVKADLAS
                     MPELKEWVANIFMIQHTPFNRIFEFLPTIPPLAQISPLWSIAVEWWLYTLFGIAFFFH
                     KSSFANRLIMSILIIPALLVAGYFTLKEYVALVWFLGSGCAYHFCNINRKYNNHGILM
                     LSLITGAAFLVRFHVLKHSLMNMYDLQLVIPGCIFLYSLLLLLSTNKLSKKIELISAF
                     LAFISYTLYLSHEPIRRVVSTFIEPTNLKRGFLICGVCIACATIIAYLLENK"
BASE COUNT          378 a          174 c          162 g          273 t
ORIGIN      
        1 gtttgttctc taataaataa gcaattatag ttgcgcaagc aatacaaact ccacaaatga
       61 ggaagcccct ctttaaattc gtcggctcaa taaatgtgga tacaactctt cttattggct
      121 catggctcaa atacagtgta taagaaataa atgcaaggaa tgctgagatt aattctattt
      181 ttttgcttag tttgttagtt gacagtaata acaacagcga atacaaaaag atacagccag
      241 gaattactaa ttgtaaatca tacatattca ttagtgaatg cttcaagaca tggaatctaa
      301 ccagaaaagc tgctcctgtg attaaactaa gcattaaaat cccgtgatta ttatacttcc
      361 tatttatatt acagaaatga tatgcgcacc cagaacccag aaaccatacc aaagcaacat
      421 actcttttaa tgtgaaatat ccagcaacta gcaatgcagg aataattaat atgctcatta
      481 taagcctgtt agcaaaagag cttttatgaa aaaaaaaggc tatcccaaac aaagtataca
      541 accaccactc aactgctatt gaccacaatg gggaaatttg agctaaaggt ggaatagtag
      601 gtaaaaattc aaaaattcga ttaaaaggag tatgttgtat catgaaaata ttagcaaccc
      661 actctttaag ctcaggcatt gatgccaaat ccgctttcac taacaaaaaa actgataaaa
      721 tcaacacagg aatgtaaatg acataaattc tgcagaaacg atcaatcatg tattctgcaa
      781 atgtataggg cttctttgtt atacaatgat atgtaattaa aaatcctgag agccaaaaaa
      841 aagcatttac tgccaaggat tgaaaaggca attgtttccc aaatgaaatt acaccaggta
      901 tgcctgataa agtatgacca gccaagacta ttatagctga aaacccgcgg agaaaattta
      961 gataggttgc ttgacttgtt gtgagtt
//
LOCUS       DAXLDT010000357          966 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.359, whole genome shotgun sequence.
ACCESSION   DAXLDT010000357 DAXLDT010000000
VERSION     DAXLDT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.359"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            232..>966
                     /locus_tag="ACF2SP_003236"
     CDS             232..>966
                     /locus_tag="ACF2SP_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD2360978.1"
                     /translation="MKINDFRYMRRGKHLSELQSEEANLLAPVNQRGKGTERALLVLH
                     GFSSSPAVYRYLIPNLKNYDAILCPVLAGHAESIEAFSKSKAADWLQSAKNACELLIN
                     QYQKVDVLGLSLGGLLACQLSQSFAINHLFLLAPALKLHMRINGMLKLAQILKCLGFR
                     QLRNAAGNLITDEHAEIAYRKLPISTIIEMLTLAQQYKWLPPACSADLFLGIHDQVVA
                     SQQVEQLFCNLPNVKIHWLENSAHVLP"
BASE COUNT          307 a          197 c          166 g          296 t
ORIGIN      
        1 ttacatattt tgcaaaaaac tattgccccg aataaggcaa aaggatttac gctttaggcg
       61 tcttgtatga caaagaaaaa atagtacagt acttatacta aaataaacta atacaattat
      121 aacttttaca tttttatatt tggtaataca ctttcctctt aggaagtgta ttaccaaata
      181 aaatcaatta ttatgcaacc acatggcatg acaatgaaca atgaatactc tatgaaaatt
      241 aatgattttc ggtatatgcg ccgcggcaaa catctcagtg aactgcagtc ggaggaagca
      301 aatctccttg ctccagtcaa tcagcgtggt aaaggcacag aacgagcttt actggtattg
      361 catggtttct cttcttcacc ggcagtttac cgatatctga ttcctaattt aaaaaattat
      421 gatgccatcc tttgccctgt attggctggt cacgccgaaa gtatagaagc cttttcaaaa
      481 tccaaagcag ctgattggct tcaatcagca aaaaacgcct gtgaactctt aataaatcaa
      541 tatcaaaaag ttgatgtttt agggttatct ctgggaggat tattagcctg tcaattaagt
      601 caatcctttg ctatcaatca cctgtttcta ttagctcccg cgttaaaatt acacatgcgc
      661 atcaacggca tgctgaaatt agcccagatt cttaagtgcc tgggatttcg acaacttcgc
      721 aacgccgcag ggaatcttat caccgatgag catgctgaaa tcgcttatag aaaactaccg
      781 atttctacaa taattgaaat gctaaccttg gctcagcaat ataaatggct ccctcctgct
      841 tgctcggccg atctttttct tggaattcat gatcaagtgg ttgcttctca acaagtagaa
      901 caattatttt gtaatttacc taatgtaaaa atacattggt tagaaaattc cgcgcatgta
      961 ttgcca
//
LOCUS       DAXLDT010000358          937 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXLDT010000358 DAXLDT010000000
VERSION     DAXLDT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.360"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..98)
                     /locus_tag="ACF2SP_003237"
     CDS             complement(<1..98)
                     /locus_tag="ACF2SP_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFD2360979.1"
                     /translation="MLLHYLFIVGICVEAITGALAAGRKKMDFFGVL"
     gene            215..559
                     /locus_tag="ACF2SP_003238"
     CDS             215..559
                     /locus_tag="ACF2SP_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360980.1"
                     /translation="MNNTIDNQSQEELTHFDANTPQSIIQLDRKLREMKSEVDMKLND
                     PSSYNGDDKEVYFQKLSKLSEEINEAIKSLDALVKMVDIQDEEFKKDFYESDVMKEFN
                     ESVMKSFDKMPE"
BASE COUNT          363 a          131 c          162 g          281 t
ORIGIN      
        1 agtaccccaa aaaaatccat ttttttacgt cctgctgcca gggctcctgt aatcgcctct
       61 acacaaatcc ccacaataaa aagataatga agtaacatat gatttgctgc tatttaaaaa
      121 gacaatgata gcactaatag tacaaatgaa gaaatgctgt acttcgacta gaatttaata
      181 gtaaggtttt aattattttg gcaggagcaa attgatgaat aatacgatcg ataatcaatc
      241 gcaagaagaa ttaactcatt ttgatgcaaa tacgccccag agcatcattc aattggatag
      301 aaagctcagg gaaatgaaaa gcgaagtcga tatgaaacta aatgatcctt catcttataa
      361 tggggatgat aaagaagttt attttcaaaa actatcaaag ttatctgaag aaataaatga
      421 agcaatcaag agcttggacg cattagttaa aatggttgat attcaagatg aagaatttaa
      481 aaaagatttt tatgaaagtg atgtaatgaa agagtttaat gaatccgtca tgaaaagttt
      541 tgataaaatg cctgaatgaa aatagatttt aaaggctctt aaaataaatt ttgagagtgt
      601 aaaatagtta aatcaacaag ctcttctgca atactatttg tcgcaggaac tgtttcaggg
      661 ttaatattca aaatatccat attgagcatt tatctctccc attcgtctta tttttagtat
      721 tagtatcggg caggttattt gaaatttcaa caaatatgac catgggtaat caataaattg
      781 acatatcgca ataaaacgat ataattaaaa tatgaacgta ataaatatat taaaagcgct
      841 ttctaatgaa aagcgattac agataatcag atggctaaaa gagccagata aacatttcag
      901 ttcgtcgcac tgtgatgtga gcaaagatgg tgtttgt
//
LOCUS       DAXLDT010000359          934 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXLDT010000359 DAXLDT010000000
VERSION     DAXLDT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.361"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(43..678)
                     /locus_tag="ACF2SP_003239"
     CDS             complement(43..678)
                     /locus_tag="ACF2SP_003239"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFD2360981.1"
                     /translation="MFLQTSQKLPKGMFKACVVPRPIAWIGTKDSEGNHNLSPFSFFN
                     IVCEEPPMVMFATTGAHNSGGAKDTLKNVEDTKEFTVNLVSYGSKEAMNITSLDFSRG
                     ISEFDMAEVDHLPSRLIQTPRVKESPISLECVYHESIQLPIPIHSNLINRMVIGKVVG
                     IHLKPEILTKDKRIDTNKLNLIARVGYNSYTKVKAHFDLNRPCGCCNKLMD"
BASE COUNT          297 a          159 c          153 g          325 t
ORIGIN      
        1 ggtgtattag cctctaactt atttattatc ttattcataa ttttaatcca ttaatttatt
       61 acaacatccg caaggcctat ttaaatcaaa atgagctttg actttggtat atgagttata
      121 tccgactctt gcaatgagat taagtttatt ggtatcaatg cgtttatctt tggttaaaat
      181 ttccggttta aggtgaattc caacaacctt accgattacc attcggttaa ttaaattaga
      241 atgaattggt attggtaatt gaattgattc atgataaaca cactctagac tgataggtga
      301 ttcttttacc cgaggtgttt gaattagcct actaggtaaa tgatctacct cagccatatc
      361 aaattcgcta attcctcgcg aaaaatccag ggaagtaata ttcattgcct ctttacttcc
      421 atagctgacc aagttaactg taaattcttt agtatcttca acgtttttta gagtatcttt
      481 ggcaccaccg ctgttatgag ctcctgtcgt agcaaacata accattgggg gttcttcaca
      541 tacaatgtta aaaaaactaa aaggcgataa attatgattg ccttcagaat ccttggtgcc
      601 aatccaagca ataggccttg gaacaacaca agctttaaac attccttttg gtaatttctg
      661 cgatgtctgt agaaacatgg tgatatattc ccttatttaa atttccccaa cgctgttttt
      721 aagttgggat gcttgacgct aaagattcaa tcatgattga ctttttagta aaactatttt
      781 taaaatggat aatattaagt gttataaaaa acccttttta taatgggttt ttaacaagga
      841 tgtttgatgt ttaccacaat tgataataac gaaggtgtag tccaaacata ttggacagaa
      901 gtaagaaata agtttgaaaa agcagatccc tacc
//
LOCUS       DAXLDT010000360          931 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXLDT010000360 DAXLDT010000000
VERSION     DAXLDT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.362"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            70..669
                     /locus_tag="ACF2SP_003240"
     CDS             70..669
                     /locus_tag="ACF2SP_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD2360982.1"
                     /translation="MDIKLKIGLRIKESRKNKKFTAVQLAEVTGFSAQRISNWERGTR
                     TPRFKDAEILGSALGVSPTWLLFLDIDHKPMKDHPFQTIPIINASSKIEGYLPIPSSI
                     QDNITHDDFAFIVHDKSMSPIYNAGDLVTFCKEKQNHCDLVLIHINATEENLFRKISS
                     EDNTFICSPINPDWPQIRFESASMFQIIGWVKNGAKVFY"
     gene            complement(666..872)
                     /locus_tag="ACF2SP_003241"
     CDS             complement(666..872)
                     /locus_tag="ACF2SP_003241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360983.1"
                     /translation="MKIVSEQVINGERIATQVIDTILSSTEHNLVTLSDIFGISLNKL
                     VLKKGFSNDEILMIVKIGNSLFIN"
BASE COUNT          337 a          164 c          142 g          288 t
ORIGIN      
        1 aacaatacac acaaatataa acgataacgt ttataataaa caattatgtt tattttgcaa
       61 ggtatttata tggatataaa attaaaaata gggttaagaa ttaaagagtc gcgaaaaaac
      121 aaaaaattca ctgcagtgca gttggctgaa gtcactggtt tttcagcgca acgaattagt
      181 aattgggaac gaggaacaag aacgcccagg tttaaagatg cagaaatact cggtagtgca
      241 ttgggtgttt ctccaacatg gttattattt ttagatattg accacaaacc tatgaaggat
      301 catcccttcc aaacgatccc tataatcaat gcctcatcta aaattgaagg ttacctccca
      361 ataccttcat caattcaaga caacataacg catgatgatt ttgcttttat tgtgcacgat
      421 aaaagtatgt ctcctattta taatgcaggt gacctagtta ctttctgtaa agaaaaacag
      481 aaccactgcg atttagtact gattcatatt aacgctacag aagagaactt atttagaaaa
      541 ataagctcag aagacaatac gtttatatgc tcacctatca atccggactg gccacaaatt
      601 cgatttgaat cagcatcaat gtttcaaata ataggttggg taaagaatgg ggctaaagta
      661 ttttattaat taatgaataa agaatttcct atctttacaa tcattaagat ctcatcattt
      721 gaaaacccct ttttcagaac aagcttattt aaagatatcc caaatatatc ggataatgta
      781 acaagattgt gttctgtact gcttaagatt gtatcaataa cctgagtcgc aatacgctct
      841 ccattaataa cctgttcact tacaatcttc atgattaaaa tccttattac gacaacaaca
      901 acgtacttta cttatttgac aagcttgatt t
//
LOCUS       DAXLDT010000361          924 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXLDT010000361 DAXLDT010000000
VERSION     DAXLDT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.363"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            155..349
                     /locus_tag="ACF2SP_003242"
     CDS             155..349
                     /locus_tag="ACF2SP_003242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360984.1"
                     /translation="MCYGEWHTIYTRFKRWSENGLFWFLVYQLQQKKKITVDINWVDS
                     TTISIHRHDSGALKKTDLNP"
     gene            445..762
                     /locus_tag="ACF2SP_003243"
     CDS             445..762
                     /locus_tag="ACF2SP_003243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360985.1"
                     /translation="MKEFSKLWSQCDWRGVQYIIADKGYDYSAVRTQITASGKTTVIP
                     RRGAICPGVGDKERYKTRSAIEQFFGKIKENKQLTLRFDKLDITFFSFFALGCLKVLN
                     LLC"
BASE COUNT          311 a          161 c          195 g          257 t
ORIGIN      
        1 atggcaataa agaagatgat tttagaaaac atattgagcc gcacatagca acacactaca
       61 agcgaccagg ccgccctgca gaagtgagtc attatcagtt ttttgcgcag tactgtatgt
      121 tttaagaacg ggagtaagtt agcgggatgt tcctatgtgt tatggggaat ggcatacgat
      181 ttatacacga tttaaacgat ggagtgagaa cggattgttt tggtttttag tgtatcaatt
      241 acaacaaaag aaaaagataa cagtagatat taactgggtt gatagtacca ccattagcat
      301 tcatcgccat gattcaggag cattaaaaaa aacggacctc aatccatagg tcgaggtcac
      361 aaaggagttg gcaccaagat tcacgtagcg ctgtcaccga ctaaaattca aggagcctgt
      421 ttgtcagcaa gtaaataagt ggatatgaag gaattttcta agctttggag tcaatgcgat
      481 tggcgaggag tgcagtatat tattgccgat aaggggtatg attattctgc agtaagaact
      541 caaattaccg cctcaggaaa aacaacggtt atccctcgta gaggggctat ttgtcctggc
      601 gtaggggata aagagcgcta caaaactcgc tctgctattg agcaattctt tgggaaaatc
      661 aaagaaaata aacaattgac cctacgcttt gataaactgg atataacttt cttttctttt
      721 tttgctttag gatgccttaa agtactcaac ttactttgtt aacagtgcca tttagttaat
      781 taaattataa tgaaaaaaga tgaaacctaa tggttagtaa cacaagcttt ttgtcaatca
      841 accaggcaaa tgcttcactg ccgtcaagac aaacagatag ataaagtttg cataaatcat
      901 aacccctaga taccctataa tgag
//
LOCUS       DAXLDT010000362          913 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXLDT010000362 DAXLDT010000000
VERSION     DAXLDT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.364"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>913
                     /locus_tag="ACF2SP_003244"
     CDS             <1..>913
                     /locus_tag="ACF2SP_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISLpn11 family transposase"
                     /protein_id="HFD2360986.1"
                     /translation="RRIRHESIGLRRSYLCIKAHKYYCPACGRYFNQRFPGIGKYQRA
                     SESLRKQVFHYHSKGVSQKDLARDLKLGKSTVERWYHYGYELRYKKIATRSCPRVLGL
                     DEHSFNRKVGYATTFCDLAKHKIFDVVEGRSEKDLEGYLNTLEGKDKVQVVCIDLSSS
                     YRKLIRRYFPNAMIVADRFHVIRLLNQFCLQTYQQIDPEMKYQRGLLAALRTNPNNLT
                     VKRLNRRERYLQQQPVIAAIYYFKQRLHRLLMRKHRTAKQCTRLIPLFLKLVASLKES
                     PFDSLKTLGKTLYQWREEVVRMWRFTKN"
BASE COUNT          276 a          164 c          207 g          266 t
ORIGIN      
        1 aagacgcata cgtcatgagt cgattggatt aagacgcagt tacttgtgta tcaaggctca
       61 taaatattat tgtcctgcgt gtggtcgcta ttttaatcag cgttttcctg ggataggcaa
      121 gtatcaaaga gcgagcgaga gtttgcgtaa acaggtattt cattatcaca gcaaaggagt
      181 gagtcaaaag gatttggctc gtgatttaaa gctgggtaag tcgactgtag agcgctggta
      241 tcattatggt tatgagcttc gctataagaa gatagcgaca cgttcctgtc cccgagtatt
      301 aggacttgat gaacattcat ttaatcggaa agtgggttat gccacaacct tttgtgactt
      361 ggccaaacat aaaatatttg atgtggttga ggggcgttca gaaaaggatt tagaaggtta
      421 tctaaacacg ttagaaggaa aagataaggt acaggtagtc tgtattgatt taagttccag
      481 ttatcgcaag ttaatcaggc gttactttcc caatgcgatg attgttgccg atcgctttca
      541 tgtgatacgt ttgttgaatc aattctgttt acagacttat cagcagattg accctgagat
      601 gaaatatcag cgaggtctat tggcagcact gagaaccaat cccaataatc tcactgtaaa
      661 acgactcaat agacgggaac gttatttgca acaacagcct gtcattgctg caatatatta
      721 ctttaaacag cgattacatc gattactcat gcgaaaacat cgcaccgcaa agcagtgtac
      781 gcgtttaatc cctctctttc ttaaactggt tgccagtttg aaagaaagtc ctttcgactc
      841 actaaagact ctaggtaaaa cattatatca atggcgggaa gaagtggtta gaatgtggcg
      901 gtttactaaa aac
//
LOCUS       DAXLDT010000363          904 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXLDT010000363 DAXLDT010000000
VERSION     DAXLDT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.365"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            2..388
                     /locus_tag="ACF2SP_003245"
     CDS             2..388
                     /locus_tag="ACF2SP_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360987.1"
                     /translation="MIFKSISSLLLGLLLVSCSNELPPGEYDANEIGKIKKVIPGVII
                     SKRPVNLHRQNADANLHVKGGADSNLIDGGYNRTHGVEYVIKLNSGTIISVVQAEDLK
                     LKNKQKILVIYGRHTRVVADNGSDVY"
     gene            complement(522..>904)
                     /locus_tag="ACF2SP_003246"
     CDS             complement(522..>904)
                     /locus_tag="ACF2SP_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HFD2360988.1"
                     /translation="KNLNSDFVVLFLINFTWIIAYDTMYAMTDKADDLKIGVKSTAIY
                     FASYDRLIIALLLIFLHSLWLVWAINKNAEWFFYLLWCTAAGILTYQLKLIYARIPKN
                     CFKAFLVSGYYGLVMWFAVGLALI"
BASE COUNT          319 a          159 c          143 g          283 t
ORIGIN      
        1 aatcatattt aaatccattt catcattact attgggtttg ctacttgtaa gttgtagcaa
       61 tgaacttcct cccggcgaat atgatgcaaa tgagataggc aaaataaaaa aggttattcc
      121 gggtgtgatt atttccaaac gaccagttaa tttacatcgt caaaacgctg atgccaatct
      181 gcatgtaaaa ggtggtgctg attctaattt gatagatggt ggttataata gaacacatgg
      241 tgtcgaatac gtcatcaagt taaactctgg caccattatt tcagtggtac aagcagaaga
      301 cttgaaatta aagaacaagc aaaaaattct ggtaatttat ggaagacaca ctcgagttgt
      361 agctgataat ggaagcgatg tatattaatt agcctgcctt ctttaaaata tgggttggtg
      421 ttggattttt tcttctaaag tctgtcttat gaaggaattt tccgacaccc acaatctctt
      481 ggcacaacaa attttctatt agggttgaga ggacatatta tttaaatcaa tgctaatcca
      541 acggcaaacc acatcactaa tccataataa ccactcacta aaaatgcttt aaaacaattt
      601 ttaggtattc ttgcataaat taattttagt tgataagtca atattccagc agcggtacac
      661 cataacaaat aaaaaaacca ttctgcgttc ttatttattg cccataccaa ccataaagaa
      721 tgcagaaaaa tcagtaacaa ggcaataatt aatctatcat aacttgcaaa atatattgca
      781 gtcgatttga ctcctatttt taaatcatcg gctttgtcag tcatcgcata catcgtgtca
      841 taagcgataa tccaggtaaa attaattaaa aacaatacaa caaaatcgct atttaaattt
      901 ttcc
//
LOCUS       DAXLDT010000364          904 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXLDT010000364 DAXLDT010000000
VERSION     DAXLDT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.366"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            1..>904
                     /locus_tag="ACF2SP_003247"
     rRNA            1..>904
                     /locus_tag="ACF2SP_003247"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          266 a          164 c          292 g          182 t
ORIGIN      
        1 agagaagcgc aaacggtgga tgccttggca gtaagaggcg aagaaggacg tggaatcctg
       61 cgaaaagcta tggggagctg gaaacgagcg ttgagccata gatgtccgaa tgggggaacc
      121 cggctgcagc gatgcggtca tttgcatttg aatacatagg atgcaaaggc gaactcgggg
      181 aactgaaaca tctaagtacc cgaaggaaaa gaaatcaaga gagattctcc aagtagcggc
      241 gagcgaacgg ggaggagcct ggcgtgattt attattgaac tgagtagaac aatttgggaa
      301 agttggccgt agagggtgaa agccccgtat acgaaggttt gatgaggaac taggcacgcg
      361 aacaagtagg ccgggacacg tgaaatcctg gttgaagatg ggtggaccat catccaaggc
      421 taaatactac ttactgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagaaccc
      481 cggagagggg agtgaaatag aatctgaaac cgtttgcgta caagcagtgg gagcatagtt
      541 taggctgtgt gactgcgtac cttttgtata atgggtcagc gagttacttt cagtggcgag
      601 gttaactgaa taagggagcc gtagagaaat cgagtctgaa tagggcgata gtcgctggga
      661 gtagacccga aaccgggcga tctagccatg tgcaggatga aggttgggta acaccaactg
      721 gaggtccgaa ccgggtaatg ttgaaaaatt atcggatgac gtgtggctag gagtgaaagg
      781 ctaatcaagc ccggagatag ctggttctcc ccgaaagcta tttaggtagc gcctcgtgaa
      841 tgattactgg gggtagagca ctgtttcggc tagggggctg tcatggctta ccaaaccgat
      901 gcaa
//
LOCUS       DAXLDT010000365          902 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.367, whole genome shotgun sequence.
ACCESSION   DAXLDT010000365 DAXLDT010000000
VERSION     DAXLDT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.367"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>902)
                     /locus_tag="ACF2SP_003248"
     CDS             complement(<1..>902)
                     /locus_tag="ACF2SP_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD2360989.1"
                     /translation="LLPIIILEHLVACFCLNISAYFKKVNYFQYEQIGEFISCYYWGC
                     LFGSLIGGALTLYYKTSKISGWGLMLLGVCLFGLMNSISLWLIAISMFLVGFIGTITA
                     TANLTSLIRSVKDNEKLKLKVISLDLIVFNLCFSFVTFVLLDLSPLYILKYVQIMICL
                     LVFVGIGSLIFYQDSIFNPPKLGSLGFKIILPERKQEFMLLMSMVLFFGLIFSMVKVV
                     FAPTLIERFGSNMMSVIAASINPWVVFLIQPLIVGRIKNSNSIWFLGIGGLIVGLSYF
                     MFGMVNSFELTAIVLVLLTFGEMM"
BASE COUNT          366 a          172 c          104 g          260 t
ORIGIN      
        1 aacatcattt cgccaaatgt taataaaacc aatacaatag ctgttaattc aaatgaattc
       61 accataccaa acataaaata acttagacct acaattaacc ccccaatccc taaaaaccat
      121 attgaatttg aatttttaat tctccctact atcaacggtt gtattaaaaa tacaacccat
      181 ggattaattg atgcggctat tactgacatc atattgctgc caaagcgttc aattaaggtt
      241 ggggcaaaaa ccacttttac cattgaaaaa ataagaccaa aaaacaaaac catgctcatt
      301 aaaagcataa attcttgttt acgctcaggt aatataattt taaaacccaa agatcctaat
      361 ttaggtggat tgaaaattga atcttgataa aaaatcaatg atccaatccc aacgaaaact
      421 agcaaacaaa tcatgatctg cacatatttc aatatataca atgggctaag atctaataac
      481 acaaatgtca caaatgaaaa acatagatta aatacaataa gatccaaact aataaccttt
      541 aactttaatt tttcattgtc tttaacagat ctaattaagc ttgtaagatt agcagtggct
      601 gttattgttc ctatgaagcc cactaaaaac atcgatatgg caattagcca caaacttatt
      661 gagttcatta ggccaaataa acaaacccct aaaagcatta atccccaacc agagattttg
      721 ctcgtcttgt aatatagtgt taatgccccg ccaataagtg atccaaatag acaaccccag
      781 taatagcatg aaataaattc accaatctgt tcgtattgaa aataattaac ctttttaaaa
      841 tatgcagata tatttaaaca aaagcatgca actaaatgtt ctaaaattat tattggcaat
      901 aa
//
LOCUS       DAXLDT010000366          902 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXLDT010000366 DAXLDT010000000
VERSION     DAXLDT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.368"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..821)
                     /locus_tag="ACF2SP_003249"
     CDS             complement(<1..821)
                     /locus_tag="ACF2SP_003249"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HFD2360990.1"
                     /translation="MDSLDLDFYAITRAFLFFSLIGFIVFLEYFFSFHRLGKAGFRRR
                     RISNWYLGIVNIIVALPLSTLTTAAYCEYYQIGLFNLLKVPFGLVFILWFIMYDLYVY
                     LFHRLSHATFLWHFHKIHHSDKSLNVTTSFRFHPVEYILFNALKIVLIILLGPYFFVI
                     LFSDFIQAILVFWGHSNIALNAKLEQALSRIIITPRYHVMHHTQDECRRNYAGGLTLW
                     DYLFHTQTEPLWNKDEINKLTLGVQTNTNWPEFKNLLLINKLIHFFGKPQFVYFKL"
BASE COUNT          340 a          145 c          151 g          266 t
ORIGIN      
        1 agtttgaaat agacaaattg cggtttgcca aaaaaatgga ttaatttatt tattaataag
       61 agatttttga attcaggcca atttgtattt gtttggactc cgagggttag tttattgatc
      121 tcatctttat tccaaagggg ttctgtttga gtatgaaata aatagtccca tagtgttagt
      181 cctccagcat aatttcttct acattcatct tgcgtatgat gcatcacatg atatctcgga
      241 gtaataatta ttcgagatag tgcttgctca agtttagcat tcagggcaat gttgctgtgt
      301 ccccagaata caagaatggc ttgaataaaa tcggagaata atatgacaaa gaaataagga
      361 cccaaaagga taatcaaaac tatttttaag gcattgaata aaatatattc aacaggatga
      421 aatctaaaac tcgtcgttac gttaagcgat ttgtcggaat gatgtatttt atgaaaatgc
      481 cataagaaag tcgcatgact taggcgatga aataagtaca catacaaatc atacattata
      541 aaccataaaa taaagacaag cccaaaaggt accttgagca agttaaataa tcctatttgg
      601 taatactcac aataggccgc tgtagttaag gtgcttaatg gaagggcaac tataatgtta
      661 acgataccca aataccaatt gctgattctt cgccttctaa accctgcttt acccaaacga
      721 tgaaaactaa agaaatattc aagaaatact ataaaaccaa ttagcgaaaa aaataaaaaa
      781 gctctggtaa tagcataaaa atctaaatct agagaatcca taacaaaacc ttaataaatc
      841 tggttaaaca caaagaaaaa gaagccccct tggtgcttat tccttagctt attaagttat
      901 gt
//
LOCUS       DAXLDT010000367          887 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXLDT010000367 DAXLDT010000000
VERSION     DAXLDT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.369"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..630
                     /gene="pdxH"
                     /locus_tag="ACF2SP_003250"
     CDS             <1..630
                     /gene="pdxH"
                     /locus_tag="ACF2SP_003250"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFD2360991.1"
                     /translation="LADIRRDYGELQLSEESAENDPISQFKLWFDDVLLNEKNDPTAM
                     VLSTVDEKGYPDSRVVLLKGLENGNFIFYTNYQSAKAMQIQKNPYAALNFYWPQMARQ
                     VRVRGRVKKISSEQSDAYFSSRPLKSQFSAIVSPQSQEILDRISLEDALNQLIEEYGQ
                     KPVVRPENWGGYMIIPDEIEFWQGRDNRLHDRIHYYRHGHEWTHRRLAP"
BASE COUNT          275 a          148 c          194 g          270 t
ORIGIN      
        1 cttgcggata ttagacgaga ttatggtgag ttgcagctca gtgaagaatc cgctgaaaat
       61 gatcccattt ctcaattcaa actatggttt gatgatgtat tactaaatga aaaaaatgat
      121 cccacggcaa tggttctgtc tacagtagat gaaaagggct atccagattc cagagttgtc
      181 ttattaaaag gattagagaa cggtaatttt atcttttata ccaattatca aagtgccaag
      241 gcaatgcaaa tccaaaagaa tccttatgcc gcacttaatt tttattggcc tcaaatggca
      301 agacaagtac gtgttcgtgg tcgagtaaaa aaaatcagta gcgagcaatc ggatgcttat
      361 ttctcctcca gacctttaaa aagtcagttt agtgcgattg tttctcccca aagccaagaa
      421 attctggata ggatttcttt agaagatgct ttaaatcagt tgattgaaga atatggccaa
      481 aaacccgtag tcagaccgga aaactggggt ggatatatga taatcccgga tgagattgag
      541 ttttggcagg gtagagataa cagattacat gatcgaattc attattaccg gcatggtcat
      601 gaatggacgc accgtagatt ggcaccttga ccggcagttt ttgtcatcat tcctgccggc
      661 atcattttac tattgagata tagaaggatg agttttcatg gcaggttagt taatggttga
      721 attcttttga ataagcacac gggattcgca aattttccca gatttataaa tcaggactcg
      781 cgaaaaattc caatctcttt gttaaaaata tgttgagctt tgaccttaga atgatgtgtc
      841 cgttttatca atattctgtt ggggatagat gggttagagc gggataa
//
LOCUS       DAXLDT010000368          886 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXLDT010000368 DAXLDT010000000
VERSION     DAXLDT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.370"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            166..>886
                     /locus_tag="ACF2SP_003251"
     CDS             166..>886
                     /locus_tag="ACF2SP_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360992.1"
                     /translation="MAETPKPKSQTPTPKPKPKPTDEQPVEPGPQQSTPGVGSAIGGF
                     IKKGVGKLADSIGKRFGSNAPQPSAENKPTEGQKKDDDKKDPQMEMIERYMKLVEQWQ
                     SGINKPFYWVGGKISAGASFLVDKAVEGIKNKLAESEQPKPSPNSSTQQSSSNSSSSS
                     SSSSSPSSSSSSSPSSSSTTPTPTSSSSGSDSQSSSPPTTSSSSGSDSQSSSPPTDSS
                     SSGSGSQSSSPPTDSSSSGSDS"
BASE COUNT          294 a          199 c          189 g          204 t
ORIGIN      
        1 tttgtaaatt attagctcat attgtcttta atttcgccat ttgttttact atattatatg
       61 ttaaataaaa aatatattcc tgaacagtaa actaggaaaa gtaacctcgg acaaaatctt
      121 gatgaggtac tataatttag ttatcaacag atatggtaaa ttaacatggc agaaactcca
      181 aaacccaaat cacagacacc aacgccgaaa ccgaaaccga aaccaacaga tgagcagcct
      241 gttgagccag gtcctcaaca aagtactccg ggagtgggta gtgcaattgg aggatttatt
      301 aaaaaagggg taggtaagtt agctgacagt atcggcaaac gatttggctc aaatgctcct
      361 caacctagtg ctgaaaataa gcctacagag ggacaaaaga aggatgatga caaaaaagat
      421 ccgcaaatgg aaatgattga aaggtatatg aagctggtcg aacaatggca aagtggtatc
      481 aacaagcctt tttactgggt gggtggaaaa atatcagctg gcgccagttt tctggttgat
      541 aaggcagtgg aagggattaa aaataaactg gctgaatcag agcaaccaaa accaagcccc
      601 aactcctcaa cgcaacaatc cagttccaat tccagttcga gttcaagctc cagctctagt
      661 cccagctcta gttctagttc tagtcccagt tctagtagta ctacccccac acctacttcg
      721 tcaagtagcg gcagcgacag ccagtcttca tcaccgccta caactagcag tagcagtggt
      781 agcgatagcc agtcttcatc accgcctaca gacagtagca gcagtggcag cggtagccag
      841 tcttcatcac cgcctacaga cagtagcagc agtggcagcg acagcc
//
LOCUS       DAXLDT010000369          876 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXLDT010000369 DAXLDT010000000
VERSION     DAXLDT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.371"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(25..702)
                     /locus_tag="ACF2SP_003252"
     CDS             complement(25..702)
                     /locus_tag="ACF2SP_003252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360993.1"
                     /translation="MYYKNENYLTISPSMVYGLSNKVSFLVTVPVAASYRNETSRSNG
                     LGDTYFQGEFAFYEHSNFRYVDQMTFVGGLTAPTGSVNKDPSIGFGAVSYFLGGTYNR
                     MSVDWLAIASTGLNVITKHHGMKLGNQYLYQMGLGRNIKSKTGKYIFFGLVEVNGIYT
                     GKDKFIGEVDPNSGGNLVNIIPSLWFSTKKLFFQLGVALPIIQTLNGEQNKIHYNLVA
                     TAGITFN"
BASE COUNT          298 a          162 c          153 g          263 t
ORIGIN      
        1 tattaaatac ttaatattaa acatttaatt aaatgttatt cctgctgtcg caacaagatt
       61 ataatgaatt ttattctgct ccccattgag agtttggata atggggagag ctactcctaa
      121 ctgaaaaaac aattttttag tcgaaaacca caaggaagga ataatattca caagattgcc
      181 gcctgagttt ggatcaactt ctcctatgaa cttgtctttt ccagtataga ttccattgac
      241 ttcaaccaga ccaaaaaaaa tatattttcc agttttactt ttaatatttc gacctagacc
      301 catttgatat aaatattgat ttcccaattt catgccatgg tgttttgtta tcacatttaa
      361 tccggtagaa gcaatcgcca accaatccac tgacattcga ttgtaagtgc cccctaaaaa
      421 atagctaaca gcaccaaagc cgatagaggg gtctttatta actgaacccg tcggagcagt
      481 caagcctccc acaaaagtca tttgatcaac atatctgaaa ttcgaatgtt catagaaagc
      541 aaattctcct tgaaagtatg tatcccccaa accatttgaa cgtgatgttt cattacgata
      601 gcttgcagcg acaggtactg taaccaaaaa ggaaacttta ttagataagc catacaccat
      661 ggaaggagaa atagtcagat aattttcatt tttataatac aaatagtttg gtgaaacgta
      721 aaattgtatc tgatttttat caatgagaga ttggccaaaa gaaaaaaagg accaggttgt
      781 tgagaggtgg gaagagcaaa attacccaga ttcggaggct cattttgtga ttctgaggaa
      841 gaagcttctc caattgcgaa ctttggcaat gagctg
//
LOCUS       DAXLDT010000370          873 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.372, whole genome shotgun sequence.
ACCESSION   DAXLDT010000370 DAXLDT010000000
VERSION     DAXLDT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.372"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..254
                     /locus_tag="ACF2SP_003253"
     CDS             <1..254
                     /locus_tag="ACF2SP_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFD2360994.1"
                     /translation="KVSFCENRLTIEGEKTTSKKDENKNYISREISYGRYERTISLPL
                     SADVDKATASFKKGMLWITIPKKTEARENVKTIKIEKAK"
     gene            complement(303..806)
                     /locus_tag="ACF2SP_003254"
     CDS             complement(303..806)
                     /locus_tag="ACF2SP_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360995.1"
                     /translation="MSIFKTIKNIIYNPNVYIAVVIGALFGGVSGGAVGLFSGGFIGR
                     SFKICMDCPNQLLGFNIGIFDLNMVAGAIIGMVIGAALGGAITGLITSFHVYRKPHLP
                     KTVSRDNIHEVLISALWTSIEISIGIILGAVIGSLNSPGIGSVTGALIGIILMLLTTI
                     WESRAKK"
BASE COUNT          314 a          178 c          135 g          246 t
ORIGIN      
        1 ttaaagtatc tttttgtgaa aacagattaa ccattgaagg agagaaaact acttctaaaa
       61 aagatgaaaa taaaaattat atcagtcgtg aaattagtta tgggcgatat gaacgcacca
      121 tttccttacc tctatctgcg gatgtggata aagcgactgc ctcttttaaa aagggtatgt
      181 tatggatcac aatacctaaa aaaactgaag ctagggaaaa tgtaaaaaca attaaaattg
      241 aaaaagcaaa ataattaatt cagtcagggt caggctccaa agcgatggag tcagccccgt
      301 tcttattttt tggctctact ttcccaaatg gtagttaata acataagaat tatgcctatg
      361 agagcccctg tcactgaccc aatacccggt gaatttaaac ttcctatgac agcgcctaaa
      421 ataattccaa tggatatttc aatactagtc cataatgcac ttattaatac ttcatgtatg
      481 ttatcgcgag aaactgtctt aggtaaatga ggctttctgt aaacatgaaa gctagtaatc
      541 aaaccagtaa tcgccccacc caaggcagcc cctataacca ttccaataat agcacccgct
      601 accatattga gatcaaaaat acctatatta aaacccaata actgattagg acagtccata
      661 catattttaa acgagcggcc aatgaatcca ccggaaaata gcccaacagc gccaccactt
      721 acccctccaa atagtgcccc aattaccaca gcaatataga cattagggtt atatataatg
      781 ttctttattg tcttaaagat actcatattc aaatgacatt cttgagttag ttaaataatt
      841 caatagcata gctcatgttt cttttatgtc aca
//
LOCUS       DAXLDT010000371          872 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXLDT010000371 DAXLDT010000000
VERSION     DAXLDT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.373"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            336..425
                     /locus_tag="ACF2SP_003255"
     tRNA            336..425
                     /locus_tag="ACF2SP_003255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:370..372,aa:Ser,seq:gct)
     gene            432..508
                     /locus_tag="ACF2SP_003256"
     tRNA            432..508
                     /locus_tag="ACF2SP_003256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:466..468,aa:Arg,seq:acg)
     gene            complement(548..655)
                     /locus_tag="ACF2SP_003257"
                     /pseudo
     CDS             complement(548..655)
                     /locus_tag="ACF2SP_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          264 a          185 c          143 g          280 t
ORIGIN      
        1 cacaccaaaa ataaactctg gttcaaaatt ttctccaaaa tcagagtttt cttttaataa
       61 tttgaataaa tggcttataa ataacctggc tttttctttg tttgatgggc aaattgtttc
      121 acctaaatat tccaatagag tgtcatacca cataataatc ctttaaatcc ctttaatagg
      181 tagtgtcata acataaatac attttaaaaa aaatctactg atatttttat atgcattaat
      241 gatatttatt atgctcaatt ttgaatttca tcactttatg caataagaat tattgacgca
      301 tgttaaatac ctgatatact ccgctaccat tgattggaga gatggccgag tggtcgaagg
      361 cgctcccctg ctaagggagt atgggagaaa tcccatcgag ggttcgaatc cctctctctc
      421 cgccaatcaa cgcgcccgta gctcagttgg atagagtatc tggctacgaa ccagggggtc
      481 ggaggttcga atccttccgg gcgcgccatt tttaatggct ctttcccaat tacggggcac
      541 tcaccattta acaacacaac accctaacac gagttctata attatcaaag ttacgaaagc
      601 cataagctcg tctttgcatc aatttcatct ttctgtgaaa gccttctgta atgccccctt
      661 atttggggta gagccaaaat gatttaaaac caagaatgta tatttttaat ttcgaaattg
      721 tggtgatttt cgttaaagta gctgactcta ctcatttcgt aagtcctgac tcagatgggc
      781 tcttccccat ttccgggggc actaatcaag aacacaaaac tttaaccctt aacctataat
      841 tttcaaaatt tctaaatcca taagcacgtc tt
//
LOCUS       DAXLDT010000372          872 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXLDT010000372 DAXLDT010000000
VERSION     DAXLDT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.374"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            121..780
                     /locus_tag="ACF2SP_003258"
     CDS             121..780
                     /locus_tag="ACF2SP_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFD2360996.1"
                     /translation="MEQWHQFTLLFPLIILVFISLISPGPDFAITVKNSLAYSRRSGL
                     LTALGIALGSLVHISYTLLGLGLFITKTPIILHIIQYLGAGYLFYIGLKNLGAKPNAD
                     QIGSYATYNDLKPSEAFFNGFITNLLNPKAMLFFISLFSVLMPREISISNKLFLAIIL
                     FLQTLLWFSFVAFSLSGKNTRTKFQRVSYWIERITGVILIALALKLLLSQLGSVDISP
                     A"
BASE COUNT          274 a          151 c          150 g          297 t
ORIGIN      
        1 tttagggagt taaaataaga taaacaatgt cttattaact aaatgtaaaa ttaaaaaaga
       61 gcgcttaaaa attagctatg atattggctg aaaaaactta gtaaaaaact tggagtctct
      121 atggaacaat ggcatcaatt tactctacta ttccctttaa ttattttagt ttttatttcg
      181 ttaattagtc ctggaccaga ttttgctatc acagtcaaaa acagcttggc ttattccaga
      241 aggagtggtt tattaaccgc gttaggaatt gctttgggca gtttggttca tattagttac
      301 actctccttg gattaggcct gtttattacc aaaacaccta taatactgca tatcatccaa
      361 tatctagggg ctggatacct cttttatatt ggccttaaaa atcttggtgc caagccaaat
      421 gctgaccaaa taggaagtta tgcaacgtac aacgatttaa aaccaagtga agcttttttc
      481 aatgggttta taacgaattt attgaacccc aaagccatgc tcttttttat cagtttgttt
      541 tcagtactta tgcccagaga aatttcaatc tccaataaat tattcctagc aattatcctt
      601 tttctacaaa cattgttatg gttttctttt gttgcattca gtctttctgg aaaaaatacg
      661 cgtacgaaat ttcaaagagt tagctattgg attgaacgta ttacaggtgt cattttaatt
      721 gcattggcat taaagttact attaagccaa ttagggtctg ttgatatttc gcccgcttga
      781 gtcgaaaagc cttgattttc gagcaccgta gcgaagcaca gaaaatcaag gcttttcggc
      841 caagatagta gaaatgtcaa cagaccttag tc
//
LOCUS       DAXLDT010000373          852 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.375, whole genome shotgun sequence.
ACCESSION   DAXLDT010000373 DAXLDT010000000
VERSION     DAXLDT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.375"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..564
                     /locus_tag="ACF2SP_003259"
     CDS             <1..564
                     /locus_tag="ACF2SP_003259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2360997.1"
                     /translation="SKQIISVSKSNNGELTSGISPDIFEKEFLPFLDLKSLLNLRLSS
                     TELYSLVTQFLGKNPKAKDLGVLALPKGSKLFAVGDNVVYLKPPKSLIFDDFYGKKVK
                     VSNDDIKKSFSSKTTFFSKKEDAERYIEQQTKINDYKMVMEKPHLGYVTLKDKVTLAK
                     MNKKDDGFTEPMSIQDAQEQNVTISPK"
BASE COUNT          313 a          148 c          131 g          260 t
ORIGIN      
        1 agtaaacaaa taatctcagt aagtaaatct aataacggtg agttaacatc aggcattagc
       61 ccagacattt ttgaaaaaga atttttgccg tttcttgatt taaagtcact cttgaacttg
      121 cgtctcagct cgacagaatt gtatagccta gtcacacaat tccttgggaa aaatcccaaa
      181 gcaaaagatt tgggtgtttt ggctcttcct aagggaagca aattgtttgc tgttggtgat
      241 aatgtagtgt atctaaagcc ccctaaatct cttatttttg atgactttta cggcaaaaaa
      301 gtaaaagtat ctaatgatga tattaagaag tcattcagct caaaaaccac attctttagc
      361 aaaaaagaag atgctgaacg ttatatcgaa caacaaacca aaatcaatga ttataaaatg
      421 gttatggaaa aaccccatct tgggtatgtt actttaaaag ataaagtaac tcttgctaaa
      481 atgaacaaaa aggatgatgg atttactgaa ccaatgtcta tccaagatgc ccaagagcaa
      541 aatgtaacaa tttctccaaa ataaaagtgt tacgccgcct tcgccaaggc ggcatcactg
      601 tcaaagaaca tgatttccca aaaaataatt ctattcatta acactgttgc tgattgcgct
      661 tggattctcc cctaataaac taaatcttac tcactttgga aaaataggat attaaaataa
      721 ctaacattct atctagtaga aaaattaagc ctatttcaca taaacaaata aaatgactcg
      781 ttttaagagt aatttatata caatatacct aataatatgg actcattttt agtcgggtag
      841 cttttttcat tt
//
LOCUS       DAXLDT010000374          840 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXLDT010000374 DAXLDT010000000
VERSION     DAXLDT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.376"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>840
                     /locus_tag="ACF2SP_003260"
     CDS             <1..>840
                     /locus_tag="ACF2SP_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFD2360998.1"
                     /translation="LSIKPPAYDNNKCYLTMKRMPGMDLNRIFDKLMANPHYLTDNQR
                     IKITYALLLALKNQVIDKGIIHRDIKPENIHVNVVDPVEVNIFDYGLAKNAGEEDNLF
                     PGSANWAAPEMFSGLKHDNKADIFSMGRVIAFLWGESVANYLSNNPLNKSRKAAKNLN
                     LPARVKWVEPFLKQMLAERQEDRIDIDSAINEFKQLASINAPKLKLSQSSIKNHSNIN
                     NLRFFQNPTKSTNNSHPTQLNIQQDIKRDLQELLENLPSEFSHYRSNIIFKHTIGRFT
                     TLQ"
BASE COUNT          303 a          145 c          148 g          244 t
ORIGIN      
        1 atttaagtat aaaaccgcct gcctacgata ataataaatg ttatctgacg atgaaacgaa
       61 tgccggggat ggatttaaat cgtatcttcg ataaattaat ggctaatcct cattatctta
      121 cagataatca acgaattaag ataacctatg ctttattatt ggctttgaaa aatcaggtca
      181 ttgataaagg aattattcac cgagatataa agcctgagaa tattcatgtt aacgtggttg
      241 accctgttga agtgaatatt ttcgattacg gattagccaa aaatgcaggt gaagaggata
      301 acctatttcc tggaagtgca aattgggcag cgcctgaaat gttttctggc ttaaagcatg
      361 ataataaagc tgatattttt tcaatgggaa gagtgattgc cttcctatgg ggggaaagtg
      421 ttgcaaacta tttgagtaat aacccactta ataaaagccg aaaagccgca aaaaacctta
      481 atttgcctgc acgagttaaa tgggtagagc catttttgaa acaaatgtta gcggaaagac
      541 aagaggatag aatagacatt gattccgcaa taaacgaatt caagcaactt gcatccatta
      601 acgcgccgaa gcttaaatta tctcagtcct caataaagaa ccattcaaat attaataatc
      661 tccgcttttt tcaaaatcca actaaaagca caaacaactc tcatcctaca caattaaata
      721 ttcagcagga tattaaaagg gatttgcagg aattattaga gaatcttcct agtgaatttt
      781 ctcattatcg cagtaatata atttttaaac acacgattgg tcgtttcaca acactacaaa
//
LOCUS       DAXLDT010000375          838 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXLDT010000375 DAXLDT010000000
VERSION     DAXLDT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.377"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..678)
                     /locus_tag="ACF2SP_003261"
     CDS             complement(<1..678)
                     /locus_tag="ACF2SP_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HFD2360999.1"
                     /translation="MLVGSIWFIKDRLQTLMLVQDIRRFLDELRESKTILPCDKRLSA
                     ILQEHFSHRESHEELTSNDIQFVLQCFSERWIADSECDYLLYPSQANQVWIKLAHEIE
                     PFTDKNYLQILLPHITNQFDFNNLTPLTETVRLENFYLGYDGKTLYRKRGLCERLLDN
                     QFELSTCRTLKTKQCEVMTIEELTRLYKGKYCNGEFSIDKEKFDNFWDFLYKKAFPRM
                     QSRGEIPL"
BASE COUNT          292 a          145 c          124 g          277 t
ORIGIN      
        1 taatggaatt tcacctcttg attgcattcg gggaaaagct tttttgtata gaaaatccca
       61 aaaattatca aatttttctt tatcaataga aaattctcca ttgcaatatt ttcctttata
      121 taacctcgtt aattcttcaa tagtcattac ttcacactgt ttagttttta gtgttctgca
      181 agtggacaac tcaaattgat tatcaagcaa acgttcacat aatcctcttt ttctatataa
      241 agtttttcca tcatatccaa gataaaaatt ttccaatcga acggtttcag tcaatggagt
      301 taaattatta aaatcaaact gattggttat gtggggcagc aaaatttgca aataattttt
      361 atctgtgaat ggttctatct catgagccaa tttgatccaa acctggtttg cctgagaagg
      421 ataaagcaga taatcacact ctgagtcagc aatccagcgc tcactaaaac attgcaaaac
      481 aaattgaata tcattggagg ttaactcttc atgactttct ctatgggaga aatgttcttg
      541 taaaatagcg gataatcttt tatcacaggg aagaatagtt ttcgattccc tcaactcatc
      601 aagaaaccta cgaatgtctt gtactaacat taaagtctgc agcctatcct taataaacca
      661 aattgaacca acaagcattc tctatcaata ctattgaact ggaataacct aaacaatgtc
      721 atgcttgcta ataaatagga attattttga tatcagattg tactttggtc aatagaggat
      781 ttaattaata gaaaaaaaca tgtgaaaata aaagaagcgg cttgtaagcc gctataag
//
LOCUS       DAXLDT010000376          832 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXLDT010000376 DAXLDT010000000
VERSION     DAXLDT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.378"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(89..>832)
                     /locus_tag="ACF2SP_003262"
     CDS             complement(89..>832)
                     /locus_tag="ACF2SP_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family alpha/beta hydrolase"
                     /protein_id="HFD2361000.1"
                     /translation="KNRSIKIILMIFIGITTFVKAYAYSPDTSNPIQINRSLTEVNSR
                     HPGEVIVLIHGLMRTYVSMKPLKAYLEAQGYQVYLYKYPSARYNIQEHGVYLNQFITT
                     ILENNPAIKIHFVTHSLGGIIVREALSKLSQNQLKHVGYLIMLAPPNQGSKLAKLSIK
                     LFPMISYFIKPLAELSSEQTSYVHRVPVPDIKMGIIAGRFDAKVPPDYARLQGQTEQV
                     IINNTHTFIMYDSKVKKLVLSFLEKGKFE"
BASE COUNT          267 a          147 c          147 g          271 t
ORIGIN      
        1 tcaattttta cacctggatt tctgacaata aaagctggat agaatactta attaagccaa
       61 tactagcctc tgactaaaac taaaaaaact attcaaattt ccctttttct aaaaagctca
      121 gcacaagttt ttttactttt gaatcataca taataaaagt atgggtattg ttaataataa
      181 cctgctctgt ctgtccttgc agacgagcat agtctggagg aactttagcg tcaaaccgcc
      241 cagcgattat ccccattttg atatcaggta caggaacacg atgcacataa gatgtttgct
      301 cagaacttaa ttcagccaaa ggttttataa agtaggaaat catcggaaat agtttaatag
      361 ataattttgc cagcttcgaa ccttgattag gaggagccag cataatgaga taacctacgt
      421 gttttaattg attttgagat aatttggata acgcttccct tacaataatg ccaccaaggc
      481 tatgggtaac gaaatgaatt tttattgccg gattattctc aagtatcgtt gtaataaatt
      541 gatttaaata aactccatgc tcttgaatat tgtatctggc ggaggggtat ttgtacaaat
      601 agacctgata tccctgagcc tccagatagg cttttaatgg cttcatactc acataggtac
      661 gcattaaacc atgaattaat acaataacct ctccaggatg cctggagttt acttctgtta
      721 atgaacggtt tatctgaatt ggattactgg tatcagggga gtaagcataa gccttgacaa
      781 atgtggttat tccaatgaat atcatcagaa ttatttttat gcttcgattt tt
//
LOCUS       DAXLDT010000377          819 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXLDT010000377 DAXLDT010000000
VERSION     DAXLDT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.379"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(139..807)
                     /locus_tag="ACF2SP_003263"
     CDS             complement(139..807)
                     /locus_tag="ACF2SP_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD2361001.1"
                     /translation="MIDTIIFDFDGVILNSEPMHFEALVQVLNQSGINLAYEEYMTHY
                     LGLSDISVFPKILNDKGLAFSSTEIRQLIERKVTVYNELIENSEQLPMTPDLDWFLVR
                     AAKQYGKIGICSGSNRHSIIKILEKIHCGRLACYFKTIVSCEDVSLGKPSPEGYLLAA
                     HHLQSNPENCLVIEDSEHGVAAAKAGGMLVAGLLTTLSRDQLANADMIVHDFKELAHL
                     LNSH"
BASE COUNT          238 a          177 c          139 g          265 t
ORIGIN      
        1 ggattttgat taaaatttat aagcgctttt acaaaaaaat tatctcgctc ttcttatctt
       61 cagaattttg tagctcatta tttttctaat aacattaagt atagtcttta taagacctct
      121 tgcatgacct gtctcttttt agtgggagtt caacaaatga gccagctctt taaaatcatg
      181 cacaatcata tcagcattgg ccagctgatc cctgctcagt gttgtgagta aacccgcaac
      241 cagcattcct cccgctttcg cagctgctac tccgtgttct gagtcctcta taaccaaaca
      301 attttcagga ttggattgca aatgatgcgc tgcgagcaaa taaccttctg gagacggttt
      361 acccaagctt acatcttcac aacttacaat ggttttaaaa tagcaagcca agcgaccgca
      421 atgaattttt tcaagaattt ttattattga atggcgattg gatccgctgc agatgccgat
      481 ttttccatat tgtttcgccg ctcgaactaa aaaccaatcc agatcaggag tcataggcaa
      541 ttgctcactg ttctcgatta actcattata aactgtgacc ttgcgctcta taagttgacg
      601 tatttcagtg gatgagaacg caagaccctt atcattcagt attttgggaa acacactaat
      661 atcgcttaat cccaggtaat gggtcatata ttcttcataa gctagattaa tcccagattg
      721 attcagtacc tggactaacg cttcaaaatg catgggttca ctgttaagta ttacaccgtc
      781 aaaatcaaag attattgtat ctatcatgta ttgcctcag
//
LOCUS       DAXLDT010000378          811 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.380, whole genome shotgun sequence.
ACCESSION   DAXLDT010000378 DAXLDT010000000
VERSION     DAXLDT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.380"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(141..431)
                     /locus_tag="ACF2SP_003264"
     CDS             complement(141..431)
                     /locus_tag="ACF2SP_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD2361002.1"
                     /translation="MKALTLNEFIDDKINQDEEFAKHYEREQIINNIAVMIVNARKKR
                     HMTQSELANKIGTKQSVISRLESGNSSFIPSLETLVKVADALNMHLKLQLQA"
     gene            complement(409..762)
                     /locus_tag="ACF2SP_003265"
     CDS             complement(409..762)
                     /locus_tag="ACF2SP_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFD2361003.1"
                     /translation="MKIKFYQKQSGKNPVAEFLNDLPSDEIARLAGCLKSVEELGFDS
                     PRVQFRQIKGSLWEIKIKTSRSGYRFFYVCIQKEIIVLLHAYKKQSQKAPKQEIELAE
                     KRMMEVYENESTYLK"
BASE COUNT          242 a          146 c          131 g          292 t
ORIGIN      
        1 aatgtaatca acgttaaata actggcctat atttgaccac aacgttgaat cgctaataat
       61 gaaggttcag agaattaaat taaaagccca tgaatattca tggcttgttt tagataatat
      121 gtatttacca attaatccaa ttaggcctgt aattgcaatt ttaagtgcat gttcagcgca
      181 tcagcaacct tcaccaaggt ctctaatgag ggaataaatg aactattgcc actttcaagg
      241 cgagaaataa ccgattgttt ggttccaatt ttattggcca attcagattg tgtcatatgt
      301 cgctttttac gggcatttac aatcattact gctatattat taataatttg ctcacgttca
      361 tagtgcttgg caaattcttc atcttgattg attttatcgt caataaattc atttaaggta
      421 agtgctttca ttctcgtata cctccatcat tcgtttttca gccagttcaa tttcttgctt
      481 tggcgctttt tgtgattgtt ttttataggc atgtaaaaga acgatgattt ctttttggat
      541 gcacacataa aaaaaccgat aaccactgcg cgatgtttta attttaattt cccaaagcga
      601 gccttttatt tgcctgaatt gtacccttgg gctatcaaaa cccagttctt caacactctt
      661 taaacaacca gcaagtcgcg cgatttcatc ggacggcaaa tcatttagaa attccgccac
      721 ggggtttttg cccgattgtt tttggtaaaa ttttattttc ataataattt atagcataaa
      781 tgtgataatt tggaaagtat ttaactaaaa t
//
LOCUS       DAXLDT010000379          801 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.381, whole genome shotgun sequence.
ACCESSION   DAXLDT010000379 DAXLDT010000000
VERSION     DAXLDT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.381"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>801)
                     /locus_tag="ACF2SP_003266"
     CDS             complement(<1..>801)
                     /locus_tag="ACF2SP_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361004.1"
                     /translation="GMVLDKFIEYFIEFDDKVFTYYISGPGTIFNTGMLLKNKNSRNY
                     SPNGVLKATAGARTAFMLPSINSHNGINKLSKLVNQDLTTPRNHNDHFELFKAINQHD
                     NSNWKVCLAYFSEKWVKHLLTDPAWVEIKNYMLEAKNKNDSFSVNSAYYDIFYSKAQK
                     DRNLRTSSPYLTNTAIHLIKIALGEHPGYVPATTANFLPIESIQKFISESFQLKRTPT
                     IMVPHSLVYEKEKEPVYYSLQNPTTPHFLTKKNEKVTANQEIDIIYRI"
BASE COUNT          243 a          124 c          140 g          294 t
ORIGIN      
        1 aaattctata aataatatca atttcttgat ttgcagtaac tttttcattt ttcttagtaa
       61 gaaaatgcgg tgtcgttggg ttttgtaatg aataatatac cggttctttt tctttctcgt
      121 acactagtga gtgtggaacc attattgtgg gagtcctttt caattggaat gattcagaaa
      181 taaatttctg tatgctttct ataggtagga aatttgcagt tgtagccggt acatacccag
      241 gatgttcacc aagagcgatt ttgatcaaat gtatagctgt gtttgttaaa tatgggctag
      301 atgtacgaag atttctatcc ttttgggctt tagaataaaa aatatcgtaa tatgcactat
      361 tcacactaaa agaatcattc ttatttttag cttctagcat ataattttta atttcaaccc
      421 aagcaggatc agtaagcaaa tgtttcaccc atttttctga gaagtaggca aggcaaactt
      481 tccaattact attatcatgc tggtttatag ctttaaatag ttcaaagtga tcgttatggt
      541 ttctcggggt tgttagatct tgattgacaa gtttagaaag cttattgatt ccattgtgac
      601 tgtttatgct gggaagcata aaagctgttc gtgcacccgc agtcgctttt agtacgccat
      661 ttggactata atttcttgaa tttttatttt tcaaaagcat ccctgtatta aatatggtac
      721 caggcccaga gatataataa gtaaaaactt tgtcatcaaa ttcaataaaa tattcaataa
      781 acttatcaag taccatgcct a
//
LOCUS       DAXLDT010000380          794 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.382, whole genome shotgun sequence.
ACCESSION   DAXLDT010000380 DAXLDT010000000
VERSION     DAXLDT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.382"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            373..>794
                     /locus_tag="ACF2SP_003267"
     CDS             373..>794
                     /locus_tag="ACF2SP_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD2361005.1"
                     /translation="MSTVAQYKNYYHYISDGWSKEPKEMFKFLAQKIKELPLKPGSKL
                     LDIGCATGELIHFLSKHFPQLQYTGIDVFDDLIEVCKVLQPNVNFINASALDLPFEFE
                     NQFDIITVVSVISIFDTIDLDIFWDNLFKATKDNGVIYV"
BASE COUNT          283 a          124 c          115 g          272 t
ORIGIN      
        1 aaaaaaaaca ttaaattgat acgcttcata tacgattgct aaaacagtat ttgacataaa
       61 attccttatg gttttttgtt cttagaatta tatttccatt cttcatttgg aagaacaaat
      121 tcaaatattt aattcaatgc aatcaaattg aaatagattt tcttctcctc aaatagagca
      181 attatatttt gattatggtc cttaatccac aaaactaaca tgcttctaaa ttaatcgttt
      241 actttgtagt attcataatt ttcattttgt gttaaaacac cccaacagat gacataggtc
      301 aaaatttctg ttaaaaagcg cataaacaac ctaaaataca atattccttg agggaaacgg
      361 aggttatgat ttatgtcaac tgtcgcgcaa tacaagaact actatcatta tatttccgac
      421 ggctggagca aagaacctaa agaaatgttc aaatttcttg cccaaaaaat taaagagttg
      481 ccattaaagc ctggaagcaa actattagat ataggatgtg caacaggaga attaattcat
      541 tttttgagta aacatttccc acaactgcag tataccggaa tagatgtttt tgatgacttg
      601 attgaggtat gtaaagtact acaaccgaat gtaaatttta ttaatgcatc agccttggat
      661 ctgccttttg aatttgaaaa tcaatttgat attattactg ttgtaagtgt tatttctatc
      721 ttcgatacta tcgacttaga tatattctgg gataatctct ttaaagcaac caaagataac
      781 ggagtaatct atgt
//
LOCUS       DAXLDT010000381          768 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXLDT010000381 DAXLDT010000000
VERSION     DAXLDT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.383"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            418..591
                     /locus_tag="ACF2SP_003268"
     CDS             418..591
                     /locus_tag="ACF2SP_003268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361006.1"
                     /translation="MAIAFAQVSIHSRSKGHSAVAAAAYRSGAQLYDDRIGRTYVMGL
                     GSDLALCSHCVDL"
     gene            647..768
                     /locus_tag="ACF2SP_003269"
                     /pseudo
     CDS             647..768
                     /locus_tag="ACF2SP_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          240 a          136 c          153 g          239 t
ORIGIN      
        1 agaacttcaa tcgaacatta acagcttaaa tataaaaatt gatgaaaatt taaataggaa
       61 aaaaaaaata agattcccta taaaatattt tctattcatc ttccttactt aaattcttac
      121 acaagaaatt aggggctgtt gacctttcag cctaaagtcc ctacgtgccg tgctttaccc
      181 acagtatcca tggatcccgc gcacaaggcg cgggacgtag gttttggtat gaaatgtcaa
      241 cagaccctaa aaaaaaacta tgattcttta atgtctcaag caaaaatgca aagggcgcaa
      301 tatacaaact ttcgtttgtt tgctagtatc aagattcaaa tatttaaaaa tctttaatgg
      361 acaaagattt tttcatttta aaaagcaacc gatggattca ttctctttgg ggattccgtg
      421 gcgattgctt ttgcacaagt ttctattcac tctcgttcaa aaggtcatag tgctgtagct
      481 gcggctgctt atcgttcggg tgctcagctt tatgatgatc gcattggtcg tacctatgta
      541 atgggactag ggtcagatct tgccctttgc tcccattgtg ttgatttata acttttgtaa
      601 ttcttgagta tagaggcaaa aggcaagatc tgaccctggt tttttgctaa agactctagg
      661 taaaacatta tatcaatggc gggaagaagt ggttagaatg tggcggttta ctaaaaacaa
      721 cggtattacg gaagggtttc atcggaagat gaagctgatt caaagacg
//
LOCUS       DAXLDT010000382          767 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXLDT010000382 DAXLDT010000000
VERSION     DAXLDT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.384"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..659
                     /gene="lciR"
                     /locus_tag="ACF2SP_003270"
     CDS             <1..659
                     /gene="lciR"
                     /locus_tag="ACF2SP_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-responsive two-component system response
                     regulator LciR"
                     /protein_id="HFD2361007.1"
                     /translation="VEDHKRTADFICKGFNEYQFQADAVYDGAEAIYRISEINYDAVV
                     LDVMLPIMDGWAVLEKIRELKPDLPVLMLSACDDVDSRVKGLEMGAYDYLVKPFSFNE
                     LLARVKSLLRRKPKESPILLKINDLTLNLQKHTAIRGDKKLPLTAKEFMLLTFMLKRS
                     GEVLSRTVIAEQVWDINFDTNTNIIDVAIKRLREKVDNDHPTKLIHTVRGVGYVLEIR
                     "
BASE COUNT          249 a          131 c          147 g          240 t
ORIGIN      
        1 ttgttgaaga ccataaaaga acagctgatt ttatctgtaa aggatttaat gaatatcaat
       61 tccaggcaga tgctgtatat gatggtgcag aagcaatcta tcgaatctca gaaattaact
      121 acgatgctgt tgttttagac gttatgttac ccataatgga tggatgggct gtacttgaaa
      181 aaattcgtga actaaaacct gatttacccg ttttaatgct ttctgcctgc gacgatgtcg
      241 atagtcgtgt gaaagggctt gaaatgggtg cctatgatta tcttgttaaa cctttttcct
      301 tcaatgagct ccttgcccga gtaaaatctt tactacgaag aaaacccaaa gagagcccta
      361 tcttattaaa gattaatgat ttaactctaa acttacaaaa gcatacagca atacgaggcg
      421 acaaaaagtt acctttaaca gcaaaagaat tcatgttatt gacgtttatg ttgaaacgaa
      481 gtggtgaagt gctttcaagg acagtcattg ctgaacaagt ttgggatatt aattttgaca
      541 cgaataccaa tatcattgat gtagcgatca agcgtttaag ggaaaaagtg gataatgatc
      601 atcctactaa attgatccat actgtcagag gagtaggcta tgtattggag attcgctaac
      661 tcaatgtcca ttactaaccg cttattactg atatttattg gttcaaccgt actgatattg
      721 tctattatca ctgggttaat ctatcctcca atgaaagaat tactcca
//
LOCUS       DAXLDT010000383          761 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXLDT010000383 DAXLDT010000000
VERSION     DAXLDT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.385"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..395
                     /locus_tag="ACF2SP_003271"
     CDS             <1..395
                     /locus_tag="ACF2SP_003271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361008.1"
                     /translation="FLNYIFLQLRLDRFKIFRFDNPWYYLFKGYDLERMDSNGFVIIM
                     ISAVMEIAGQCYLYRGELEEFYFDKDGSLDRLVLRNASRRKIEDDKTTNSKDNNRFYP
                     IDGDYFVLKYAKLGNLNVQFLELEELNS"
     gene            503..>761
                     /locus_tag="ACF2SP_003272"
     CDS             503..>761
                     /locus_tag="ACF2SP_003272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361009.1"
                     /translation="MDSTAAFFWNSTEKKLSYMVINSNLQTRVKPQTTGKLKELDSKT
                     LKNEISKMIKSFYDEIEKIFESSPNHNITIEWKIVNSGNPAP"
BASE COUNT          265 a          103 c          130 g          263 t
ORIGIN      
        1 agtttcttaa ttatattttt cttcagttaa gactagatcg gttcaaaatt tttagatttg
       61 ataatccatg gtactacctt tttaaaggtt atgatttaga acgtatggac tcaaatggtt
      121 tcgtaattat aatgatctct gctgtaatgg aaattgctgg ccagtgttat ttatatagag
      181 gggagcttga agaattttat ttcgataaag atggttcttt agataggctt gttctaagaa
      241 atgcttccag gcgaaaaatc gaggatgata aaacgacaaa ttcgaaagat aataatcgct
      301 tttatcctat tgatggagat tattttgtcc ttaaatacgc taaacttgga aatttaaatg
      361 tgcaattctt agagctagag gaactaaatt cttaggcagc tgttttatac ttcgctcatc
      421 agtttaatct cattctatta taactagcta actaagttga tgtgctataa ttgaacatat
      481 ctctaattgg agagttatta acatggatag tacagcggct ttcttttgga atagtactga
      541 gaaaaaatta agttatatgg ttattaatag taatctgcag acaagagtaa aacctcaaac
      601 aacaggaaaa ctcaaagaat tagattctaa aactttaaaa aacgaaatct caaaaatgat
      661 aaaatccttt tatgatgaga ttgagaaaat ttttgagtct tctcctaatc ataatattac
      721 tattgaatgg aagattgtta actcaggcaa tccagcacct c
//
LOCUS       DAXLDT010000384          742 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXLDT010000384 DAXLDT010000000
VERSION     DAXLDT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.386"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            482..>742
                     /locus_tag="ACF2SP_003273"
     CDS             482..>742
                     /locus_tag="ACF2SP_003273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361010.1"
                     /translation="MKWIFKFFKSNIAVLVLLILVLVLLVLCNISCMPLSCLPQIIQG
                     FFILLAAVVTAIFAIHKYQSEQRILRLQKIYFEDSLLGQANSI"
BASE COUNT          226 a          120 c          142 g          254 t
ORIGIN      
        1 tataagcaaa cgtaataaag cgaaacgaaa tccgggatgt tcgaagccca atatctcgga
       61 ttacggctgc gcctgtatct gggcttgctg atttgattac agggcgcttt agcactggat
      121 caaattatta gagttagaaa aaaaagttaa atcgaaattg tcccatatgc cccatcttga
      181 tttttaggtg gggaatgcct ccagaccagc aatgatgtga ttgccccatc agtcccattt
      241 gtccctgtag tttttaataa cattgtaatg tattagtaat tttagaagat tagccattta
      301 ttgtattttt gttcaaacgc cttgagtgct tatgaacatt aggatttaat atttttctgc
      361 gtcactgcac agaatgcagt gatgcagaag taaattgaat taactttaga ctatactgaa
      421 aatgtatgca ttatactcag catgagttta aatagttcaa aagggattgg agtaaaaagt
      481 aatgaaatgg attttcaaat ttttcaaatc caatatagct gtattagttt tattaattct
      541 agtgctcgtg cttcttgttt tgtgcaatat aagttgtatg cctttatctt gcctgcccca
      601 aattattcag gggtttttta ttctattagc tgctgtggtc actgcaatat tcgcaattca
      661 caagtaccag agtgagcaaa gaatactacg tcttcaaaaa atatattttg aggatagttt
      721 gctaggtcag gcaaattcaa tt
//
LOCUS       DAXLDT010000385          723 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.387, whole genome shotgun sequence.
ACCESSION   DAXLDT010000385 DAXLDT010000000
VERSION     DAXLDT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.387"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>723)
                     /locus_tag="ACF2SP_003274"
     CDS             complement(<1..>723)
                     /locus_tag="ACF2SP_003274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361011.1"
                     /translation="KLINKKTNMEFCRNFTRVCWDEIREEVKRSNPEFFFLVDEIAPG
                     KDFPLYVFNFGYGDLIGDSETFYIPHQGKLQTLNNFSSQEPITKDLFYGFHSCPLGLV
                     LDKCFEWYLRGDSENETHPVYIDKKGDFFNIGHVTQIKPLKRYLPNGILSVKAGAQTT
                     LVIQNIGCKRSHNRLIRSGIDCTIPNSYHDHSEFFKRIYQSCPAESKWRASIVYFSEK
                     WIDNIVNNPEWININKYFFKYYL"
BASE COUNT          243 a          123 c          113 g          244 t
ORIGIN      
        1 tagataatac ttaaagaaat atttattaat attgatccac tcaggattat taacaatgtt
       61 atctatccat ttttcagaga agtaaacaat agaggccctc catttacttt cagcagggca
      121 agattggtaa attcgtttaa aaaattcact gtgatcatgg tatgaattgg ggatggtgca
      181 atcaattcca gatcgaataa gacgattatg gcttcttttg catccaatat tttgtattac
      241 aagagtggtt tgtgccccag cttttacaga cagtatgcca ttaggcaagt atcgtttaag
      301 tggttttatc tgggttacat gtccaatgtt aaaaaaatcc cctttcttat ctatgtaaac
      361 aggatgcgtt tcattttctg aatcacctct taagtaccat tcaaagcact tatctaatac
      421 aagacctaat ggacaactgt gaaatccata aaagaggtct ttagtaattg gttcttgaga
      481 tgaaaaatta ttaagagttt gaagctttcc ttggtgcgga atataaaagg tttcagaatc
      541 accaattaaa tcaccatatc caaagttaaa aacatatagt gggaaatctt ttcctggcgc
      601 aatttcatca actaagaaaa aaaactcagg attagagcgt tttacttcct ctctaatttc
      661 atcccaacaa actctagtaa aattcctaca aaattccata ttagtctttt tgttaattaa
      721 ttt
//
LOCUS       DAXLDT010000386          718 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.388, whole genome shotgun sequence.
ACCESSION   DAXLDT010000386 DAXLDT010000000
VERSION     DAXLDT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.388"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..697
                     /locus_tag="ACF2SP_003275"
     CDS             <1..697
                     /locus_tag="ACF2SP_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD2361012.1"
                     /translation="QLFDQDSCTYTYLIGSTVSKHAIIIDPVRSQVSHYINLINKLEL
                     NLVASIDTHLHADHITGSGQLTILTDCHSMIGMESKAKFVHIKFHDNEILNFGNIKIK
                     AMHTPGHTPDSYCFVMEDRIFTGDTLLINATGRTDFQNGSASAQYDSLFNKLLKLPGF
                     MRIYPGHDYNQKQYSTIDDEKKNNPRLQVKSRQEYVEMMGNLNLSFPKNMHFAVPANL
                     NNGLTEQEVNALV"
BASE COUNT          245 a          115 c          123 g          235 t
ORIGIN      
        1 ccagctattt gatcaggatt cctgcactta tacttacctg ataggctcta ctgttagcaa
       61 gcatgcaatt attattgatc cggtacgatc acaagtttcg cattatatta atctaattaa
      121 caagttagaa ttaaatttgg ttgcttcaat tgatacacat ttacatgctg atcatattac
      181 tggatcaggc caattaacca tccttactga ttgtcattct atgataggaa tggaaagcaa
      241 agcgaaattt gtgcacatca aatttcatga taatgagatt ttaaattttg gaaatattaa
      301 aataaaggct atgcacacgc cagggcatac tccagattcc tattgttttg taatggaaga
      361 tagaatattt actggcgaca ctttattaat caatgctaca ggacgtactg attttcaaaa
      421 tggtagcgcc agtgcgcaat atgacagtct ttttaacaaa cttttaaaat taccgggatt
      481 catgagaata tatcctggcc atgattataa tcaaaaacaa tatagtacaa ttgatgatga
      541 gaaaaaaaat aatcccagat tgcaggtaaa atccaggcaa gagtatgttg aaatgatggg
      601 taatctgaat ttgtcttttc ctaaaaacat gcattttgca gttcccgcca acttgaataa
      661 tggattaact gagcaggaag ttaatgcttt agtttaaaga ttattatcat ttattttg
//
LOCUS       DAXLDT010000387          716 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.389, whole genome shotgun sequence.
ACCESSION   DAXLDT010000387 DAXLDT010000000
VERSION     DAXLDT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.389"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>716)
                     /locus_tag="ACF2SP_003276"
     CDS             complement(<1..>716)
                     /locus_tag="ACF2SP_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFD2361013.1"
                     /translation="RKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQID
                     AAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAA
                     DGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSYDFPGDD
                     IPIVVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISG
                     RGTVVTGRVESGIVKVGEEV"
BASE COUNT          187 a          198 c          120 g          211 t
ORIGIN      
        1 tcaacttcct caccaacttt aacaattcca ctctcaacac gaccagttac cactgttccg
       61 cgtccagaaa ttgaaaatac gtcttcaatc ggtaacaaaa atggcttgtc tatgtttcta
      121 actggctcag gaatgtatga atccattgtt tcaaccaatt tctctatagc cttaacgcct
      181 atatcactgt cttcaccttc caatgctttc aaagctgaac caacaacaat aggtatgtca
      241 tcccctggga aatcgtaact gcttaataaa tctcgcactt ccatttccac taactctaat
      301 aactcagggt catcaaccat atccgctttg ttcatgaaca caacaatata tggaacacct
      361 acctggcgag acaatagaat gtgttccctc gtttgtggca taggaccatc agctgctgat
      421 acaaccagta tcgctccgtc catttgcgca gcaccagtaa tcatgttctt aacatagtca
      481 gcatgccccg ggcagtctac gtgcgcataa tgtctgctcg ctgattcgta ctcaacgtgt
      541 gctgtagaaa tagtaatacc acgctctctt tcttctggcg cagcatcaat ttgatcgtac
      601 gccttcgctg taccaccata tttcttcgcc ataatcgttg taatagctgc tgtcaatgtc
      661 gtcttgccat ggtctacgtg accaatcgtg cccacgttta cgtgcggctt cttacg
//
LOCUS       DAXLDT010000388          706 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.390, whole genome shotgun sequence.
ACCESSION   DAXLDT010000388 DAXLDT010000000
VERSION     DAXLDT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.390"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            149..>706
                     /locus_tag="ACF2SP_003277"
     CDS             149..>706
                     /locus_tag="ACF2SP_003277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361014.1"
                     /translation="MEKNNNNGLPQALLKRLHLKKIQPLYSVWRFADESAFSSSDIYQ
                     ALEKAVSLQHKSKLKNYGIVKLSIGHHPIKKDKTQDTAHESWGHAIYLRYDPERKIAY
                     FYDPNHGRSINFYNWKKLDNDSDFKKMKKNEQDEAVLNYMLLCFSQMIANDFDDINDI
                     SCYEMEMNFNALDKLVSAGNKIIGGL"
BASE COUNT          253 a          110 c          127 g          216 t
ORIGIN      
        1 agtttgccaa gcgaatactt atcgattgat tactcgagaa atgactagcc tgggtagaaa
       61 tcaaccatta tctaattctc aatgggaagc agaagtcaaa atctcgctcc aggatcgctt
      121 caaccaagct ttatatcaag taagtataat ggaaaaaaat aataacaatg gtttgccaca
      181 agccttatta aaacgacttc accttaaaaa aattcaaccc ctctatagcg tttggcgttt
      241 tgcggatgaa tctgcttttt cttctagtga catctatcag gcgcttgaga aagcggttag
      301 tttgcaacat aaatctaaat taaaaaatta tggaattgta aaactatcaa ttggacatca
      361 tccaattaaa aaagataaaa cccaagatac agctcacgaa tcctgggggc acgcaatcta
      421 tttacgttat gatccagaaa gaaaaattgc ctatttttac gatccaaatc atgggcgtag
      481 tattaatttt tataattgga aaaaattaga taatgatagt gattttaaaa agatgaagaa
      541 gaatgagcag gatgaggccg ttctaaatta catgcttttg tgcttttctc aaatgattgc
      601 caatgatttt gatgatatta acgatataag ttgttatgaa atggaaatga attttaacgc
      661 tcttgataag ctggtttctg caggtaataa aattattgga ggatta
//
LOCUS       DAXLDT010000389          703 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.391, whole genome shotgun sequence.
ACCESSION   DAXLDT010000389 DAXLDT010000000
VERSION     DAXLDT010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.391"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..654
                     /locus_tag="ACF2SP_003278"
     CDS             <1..654
                     /locus_tag="ACF2SP_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HFD2361015.1"
                     /translation="GSLESQQLDCDQVHEEILEKVVAPFTPDKFTYNTYISCSYNPDT
                     NMATGFLINSYLDPLTDDAVELLETYLAKNNGSNLLGTPLTFEPAKALVVALTISAGM
                     KKNPNKPPFVEYKQDRSNFYFKSNYEMRNKLFTDIYQNFFTNDPNKILPFLDKWIFVN
                     ASSVYEAILMDSNFVELQPERIFLMENGEQIFVSNLKYYFTHNCNRYSNHRCLQPQA"
BASE COUNT          234 a          133 c          114 g          222 t
ORIGIN      
        1 ggttctctcg aatcacaaca gctggactgc gatcaagtac acgaagaaat tcttgaaaaa
       61 gtggtggctc cttttactcc tgataaattc acttacaata cttatattag ttgtagttat
      121 aatccggata ccaatatggc cactggcttt ctcatcaata gctatttaga tccactaacc
      181 gatgatgcag tggaattatt agaaacttac ctggcaaaaa ataatggatc caatctgtta
      241 ggtacaccat tgacatttga gccagcaaaa gctcttgttg ttgctttaac tatctcggcg
      301 ggaatgaaaa aaaatcctaa taaaccaccc tttgtagaat ataagcaaga ccgcagcaat
      361 ttctacttca aaagcaatta tgaaatgaga aataaattgt tcactgatat ttatcaaaat
      421 ttctttacca atgatcccaa taaaattcta cctttcctgg ataagtggat tttcgttaat
      481 gcctcaagcg tttatgaagc cattttaatg gactccaatt ttgtcgaact gcaacctgaa
      541 cgaatttttc ttatggaaaa tggtgaacaa atatttgttt ccaatctaaa atattatttc
      601 actcataatt gtaataggta cagtaatcac cgctgcttac aaccgcaagc ttgaagctct
      661 tttgacgaag gagacatttt tgggatgagc acattttatt aaa
//
LOCUS       DAXLDT010000390          699 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.392, whole genome shotgun sequence.
ACCESSION   DAXLDT010000390 DAXLDT010000000
VERSION     DAXLDT010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.392"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            202..636
                     /locus_tag="ACF2SP_003279"
     CDS             202..636
                     /locus_tag="ACF2SP_003279"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD2361016.1"
                     /translation="MNVVGAVVKKQVKGTKTIAGKRQVMLLPPALEALNAQMKYTFDI
                     GKWVFHNPCTDQSWETDHQIRRTAWVHTIKRAGIRYRNPYQTRHTYASMMLSGGENIM
                     WIASQMGHVDTEMVMKTYGKWIPDDSSKKGYRPINNWGTYID"
BASE COUNT          240 a          121 c          131 g          207 t
ORIGIN      
        1 tttcaaaacc atgcccaaca ctggattgtc ttttgttttg tacatatcaa ctgaatataa
       61 tactagtgaa tttattttat tagataatta tttcagtaag ttctaatatt caggactaat
      121 ttcccaagaa tattaacctc agcaatatca ctcattttaa ggcaaagagc aagatctgac
      181 cctgatgaat cccgtgggat aataaatgtt gtcggggctg tggttaaaaa acaggttaag
      241 ggaactaaga ctattgcagg caaacgccaa gtaatgctgc ttcctcctgc tcttgaagca
      301 cttaacgcac aaatgaaata tacatttgat attgggaaat gggtatttca taacccatgc
      361 acggatcaat catgggaaac cgatcaccaa atccggcgca ctgcttgggt gcatacaatt
      421 aagagagctg gtattcgtta cagaaaccct tatcaaacaa gacatactta tgcttctatg
      481 atgttatctg gtggtgaaaa tatcatgtgg attgcaagtc agatggggca tgttgatact
      541 gaaatggtaa tgaagaccta tggtaaatgg atccctgatg attcatcgaa gaaaggatat
      601 agacctataa acaactgggg aacatatatt gactaaaatg atcaatttta ccaatacatt
      661 gacaaattaa tagcattaaa tcatgataat ctttttgac
//
LOCUS       DAXLDT010000391          698 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.393, whole genome shotgun sequence.
ACCESSION   DAXLDT010000391 DAXLDT010000000
VERSION     DAXLDT010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.393"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          237 a           98 c          117 g          246 t
ORIGIN      
        1 aaagccaata atttttacat tataaatttg gccaattaat tggcggtttg tatattgttg
       61 tgctataaat atagtatatg gttagggagt tgtgtccaat ttgtgcgaac agtaatattc
      121 aaggaataaa aatattagta gctgtatctt cttaataaaa aaagatcatt taaaaagatc
      181 ttctctagtt gtaaaaccca ctcaggcttt ttacgtagaa tttctttgtc ctaaaaaaca
      241 cccctgtatt tccatatcta ttttgcaaat aagcattaat acaaaaataa gggggtatat
      301 atgacataac gaaattaaac caaggttgtt ggcttaaccc cgcaggtttc tgtataattt
      361 ctagacaaaa tgattatagg tatctgtggg tcaaaagtca actattaata aataaattaa
      421 ttaataggat taataatgac taaagatagt ctgataccac cacccagtgg ttttaaatgg
      481 gtattttgtc gtcatcgacg tgtgaagaat tcagatagat atcttgatgc acatgattat
      541 gggctcgagt gctggagatt tttaatacgt tgctaaactg cttactccgt tctatatcga
      601 acggagtata ttttccctaa tttgatttag ttatttaatg cttttaagtt cctaattaaa
      661 tattaaataa gatctgttat cctgcatgaa aacgttga
//
LOCUS       DAXLDT010000392          680 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.394, whole genome shotgun sequence.
ACCESSION   DAXLDT010000392 DAXLDT010000000
VERSION     DAXLDT010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.394"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>680
                     /locus_tag="ACF2SP_003280"
     CDS             <1..>680
                     /locus_tag="ACF2SP_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD2361017.1"
                     /translation="NENFNLNNLTLLTRADLANFILKGGYPEIQDKSSRAKQIWYKSY
                     IEGRLFKDFETLYHAKGDYRSKLEALIPYLAGISGNLLKYSNISNDLGEDDKLVKAYI
                     EVLELMFIIKRVPAYLKNKAKRQAITIPKLQTIDTGLACSLLGIKNEGQLLNSPHYGG
                     LLENLIYMELLKQNSWSEEKVELLHFRDKYQNEVDIVMERDNHQIIGVEIKASATVNM
                     HDFKGLIK"
BASE COUNT          259 a          112 c          117 g          192 t
ORIGIN      
        1 aatgaaaatt ttaacttaaa taacctaaca ctacttaccc gcgccgattt agccaatttt
       61 attctcaaag gtggatatcc cgagattcaa gataaaagct caagagcaaa acaaatatgg
      121 tacaaatctt atatagaagg tcgtcttttt aaagatttcg agactctata tcatgctaaa
      181 ggtgactatc gttctaaact tgaagccctt attccttatt tagcgggaat ctcgggcaat
      241 ctacttaaat attcaaatat ttcaaacgac ttaggcgaag atgataaact ggtcaaagca
      301 tatatcgaag ttttagaatt gatgtttatc attaaaagag tccctgctta cttaaaaaat
      361 aaagcaaaac ggcaagctat tacaataccc aaactgcaga caatagatac aggacttgcc
      421 tgcagccttt taggtataaa aaatgaaggg caattactta atagccctca ttatggaggg
      481 ctattagaaa atttaattta tatggagttg ctcaaacaaa acagttggtc agaagaaaag
      541 gtagaattat tacatttccg agataaatac caaaatgaag tggacatcgt catggaaagg
      601 gataatcatc agatcattgg agttgaaatc aaagcgtcag caactgtaaa catgcatgac
      661 tttaaaggat taataaaatt
//
LOCUS       DAXLDT010000393          670 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.395, whole genome shotgun sequence.
ACCESSION   DAXLDT010000393 DAXLDT010000000
VERSION     DAXLDT010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.395"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..344
                     /locus_tag="ACF2SP_003281"
     CDS             <1..344
                     /locus_tag="ACF2SP_003281"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2361018.1"
                     /translation="NAPFTGAKPDRYSIYVKDNDGHIIGGAIVYAHKHSIYVDVLWVS
                     EKYRSMGIGAELLRNIETEAMKRGIPESTLDTFSFQAEGFYLKQGYKHLGTIKNYLEG
                     HDRIYLRKKLN"
BASE COUNT          229 a           91 c          124 g          226 t
ORIGIN      
        1 ttaatgctcc atttacaggg gctaagccag atcgttattc aatctatgtg aaagataacg
       61 atggtcatat tattggtgga gctattgtat acgctcataa gcattcaatc tacgttgatg
      121 tcctctgggt tagcgagaaa taccgtagta tgggtatcgg agctgagctt ttacgaaata
      181 ttgaaactga agcaatgaaa agaggaattc cagagtcaac attagatact ttttcttttc
      241 aagcggaagg cttttattta aaacaaggtt ataagcattt agggactatt aaaaactatt
      301 tagaaggaca cgatcggatt tatttgcgca aaaaattgaa ttagttgtat tgattgatca
      361 aattagtttt gtaatatcct acctattttt attgcaagga tttactagat atctcatcag
      421 attcaacttc gagaagatct tcttcatatt tttcctataa taaccttagt ttgaatctgg
      481 cgcggtcacg tttgaaggtg aggtaaaatg gtatatatcc atagcggttt ttgcccagaa
      541 aaatcattat gaaagctcta tgattattag aaagacaaat ttaaagtata ctgatattct
      601 taatattaag aaaagcttgt ttagaaatat gaaatgaaaa ttattataaa agtcggaaca
      661 caaagtattt
//
LOCUS       DAXLDT010000394          660 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.396, whole genome shotgun sequence.
ACCESSION   DAXLDT010000394 DAXLDT010000000
VERSION     DAXLDT010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.396"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>660)
                     /locus_tag="ACF2SP_003282"
     CDS             complement(<1..>660)
                     /locus_tag="ACF2SP_003282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361019.1"
                     /translation="SMECCNSIIPETNPHKELIASYLKSLNLTNMRVIKTINTLSENL
                     QAIVLDLEDETWDKLIKSLCLFSYCQYIVGEHIPTLDFLIEKGKDLPKLFSSNDSGIN
                     NIKEQKEFLHKYLFYGMTNLDFIMAQGVKTGFFNEQELVAEVKNVNCQIIKDKEKEAS
                     YNIWDSLSDGFNDNEQQVIEDLKSGYFQYKQCMSINDIDYIISIFRQIQQNALAEEIM
                     N"
BASE COUNT          215 a          113 c          102 g          230 t
ORIGIN      
        1 cattcattat ttcttcagcg agagcatttt gctgaatttg cctaaaaatt gaaataatat
       61 aatcaatgtc attgattgac atacattgtt tgtattgaaa atatcccgat tttaaatcct
      121 cgataacctg ctgttcattg tcattaaagc catcagacaa agagtcccaa atattgtaag
      181 aagcctcttt ttccttatcc ttaattattt ggcagttaac attttttact tcggcaacta
      241 gctcttgttc attaaaaaat ccagttttaa caccctgagc cataataaaa tcaaggtttg
      301 tcattccata aaaaaggtat ttatgaagaa actctttttg ctccttaatg ttattaatac
      361 ctgaatcgtt tgatgagaac aatttcggca agtcttttcc tttttcaatc aaaaaatcca
      421 aggtgggtat atgctcaccg acaatgtact gacaataaga aaataaacag agagatttaa
      481 taagcttatc ccatgtctca tcctcaaggt cgagaactat cgcttgaaga ttttcagata
      541 aagtattaat tgttttaatg acacgcatat ttgtcagatt gaggctcttg agataacttg
      601 caattaattc tttatgggga ttggtttcag gaataataga gttgcaacat tccattgagg
//
LOCUS       DAXLDT010000395          659 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.397, whole genome shotgun sequence.
ACCESSION   DAXLDT010000395 DAXLDT010000000
VERSION     DAXLDT010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.397"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>659
                     /locus_tag="ACF2SP_003283"
     CDS             <1..>659
                     /locus_tag="ACF2SP_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361020.1"
                     /translation="LLIFGQEFPGSVNSLLIKACNNKNIPTLIIPFAVGTTKEIVESL
                     YDKDIFDVSSSITNYLASLLYPNWINFYKGKKMLRLTGKQIFFLELMGLAPEHPWLPN
                     NSFVTKIAVESEKMFSYYQSMKFPVHQLSLTGSLSDDILVEATTNGQEIKKKICQKMN
                     LDPEKPILLCAWPTDQFGSRFVSLEFKNYEQLCRAWAKCLKEIEREGKYSVIIRPHPV
                     T"
BASE COUNT          223 a          109 c          110 g          217 t
ORIGIN      
        1 atcttttgat ttttggacaa gaatttcccg gctctgtaaa ttctctctta attaaggctt
       61 gtaataataa aaacatccca accctaatta taccatttgc agtcggtacc actaaggaaa
      121 tagtggagag cttatatgac aaggatattt ttgatgtatc ttcaagtata actaattatc
      181 ttgcatcttt actctatcct aattggatta atttttataa aggaaaaaag atgctcaggt
      241 taactggtaa acaaattttc tttttagaac tgatggggtt agctccagaa catccttggc
      301 ttcccaataa ttcctttgtt accaaaattg ctgttgaaag cgaaaaaatg ttttcatatt
      361 accaatcaat gaaattccct gttcatcaat tatctctgac aggaagttta tctgatgata
      421 ttttggttga ggcaacaaca aacggtcagg aaattaagaa aaaaatatgt caaaaaatga
      481 accttgatcc agaaaaacct attttattgt gtgcttggcc aacagatcaa tttggttcac
      541 gatttgtttc cctggaattc aaaaattacg aacagctttg tagagcatgg gcaaaatgtt
      601 tgaaagaaat tgaaagggaa ggaaaatatt ctgtaattat tcgacctcac ccggttact
//
LOCUS       DAXLDT010000396          654 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.398, whole genome shotgun sequence.
ACCESSION   DAXLDT010000396 DAXLDT010000000
VERSION     DAXLDT010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.398"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            208..>654
                     /locus_tag="ACF2SP_003284"
     CDS             208..>654
                     /locus_tag="ACF2SP_003284"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361021.1"
                     /translation="MHTLYFKLVYNKKKYYFIQTPISLISQKNTRFDEDTKYDNKWIF
                     KKKLLDSQIPCPLGRVFISSKKAYNYGISLGFPLVVKPLSESLSIHTSCTIQTPNELK
                     EAINIVKQVDFRVLVEKHIPGDVYRSLIINDSLIACVRRQPESIVGD"
BASE COUNT          226 a          112 c          104 g          212 t
ORIGIN      
        1 ttatctaaac tctttctcct atcagagcat gattttttac gattcaataa cctattaaat
       61 cagttggtca ttaatatttt tcaaaaacta aaactatata ccaagactca gaccattgat
      121 aggaaacaat tcaataacag tgtccttgca ttttgggatg aagcacaaaa aagagggcta
      181 gagctgtata attttaagga atctaacttg catactctgt attttaagtt ggtctacaat
      241 aagaaaaaat attatttcat acagactcca atctctctta tttctcaaaa aaatacacgc
      301 tttgatgagg atactaaata tgataataaa tggatcttca agaaaaaatt attagatagc
      361 caaattccct gccccttagg gagggttttt atttccagca aaaaagccta caactatggc
      421 atttcattgg gatttcctct tgtggttaag ccattatctg aatctctaag tattcacact
      481 tcatgtacta ttcaaactcc aaatgaatta aaagaagcga taaacatagt aaagcaagtt
      541 gattttcgcg tgctggttga aaaacacata cctggggatg tttatcggtc attaattatt
      601 aatgattcat taatcgcttg tgtgaggcga caacctgaga gcattgttgg cgat
//
LOCUS       DAXLDT010000397          647 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.399, whole genome shotgun sequence.
ACCESSION   DAXLDT010000397 DAXLDT010000000
VERSION     DAXLDT010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.399"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>647
                     /locus_tag="ACF2SP_003285"
     CDS             <1..>647
                     /locus_tag="ACF2SP_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361022.1"
                     /translation="LQHKHVPYPDYNENWHAKLDPFKRILLFAQIQKIRNDHPDEDII
                     FNFIDDKDDILSTLKKYFINYSYMIPHGVTLRLNHYDGSNVSSYASIKGTGRPYKNYR
                     QIVKDMHEHVYNLDAFAETINQNDKFSEIPAVRQILEHEFFVSDPDDVVSCLDKGKIP
                     FVIRKSKSQISGYIMFTAVYKTSQGIFANRYGINTEGELYKFFDSQIEKMDIDSE"
BASE COUNT          229 a          113 c          103 g          202 t
ORIGIN      
        1 aactacaaca taaacatgtt ccttatcccg attataatga aaattggcat gcaaaattag
       61 atccctttaa aagaattctc ctttttgcgc aaatacaaaa aatcagaaat gatcatcctg
      121 atgaagatat aatctttaat tttattgatg acaaagatga cattttatcc accttaaaaa
      181 aatattttat aaattattct tatatgattc ctcatggagt tactttacgt ttaaaccatt
      241 atgacggcag caatgtcagt tcatacgcca gcataaaggg aaccggacgt ccgtacaaga
      301 attaccggca aatagtcaaa gacatgcatg agcatgtgta taatcttgat gcgtttgcgg
      361 aaacaataaa ccaaaatgat aaattttccg aaatacctgc cgtacgccaa atattagaac
      421 atgagttctt tgtttcagat cctgatgatg tcgttagctg tcttgacaaa gggaaaattc
      481 cttttgtaat tcgcaaaagt aaatcccaaa tttctggtta cattatgttt acagccgttt
      541 acaaaacctc gcaaggaatc tttgccaatc gatatggtat taatacggaa ggcgagcttt
      601 ataaattttt tgattcacaa attgaaaaaa tggacattga ttctgag
//
LOCUS       DAXLDT010000398          645 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.400, whole genome shotgun sequence.
ACCESSION   DAXLDT010000398 DAXLDT010000000
VERSION     DAXLDT010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.400"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..546)
                     /locus_tag="ACF2SP_003286"
     CDS             complement(<1..546)
                     /locus_tag="ACF2SP_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD2361023.1"
                     /translation="MVEDLMLCTNFFIFTGGPGSGKTAVLNELNTWGYLTVSEVARSI
                     IQKQQADGGDAIHTGNRKAFRDLMLEQSIADYCKMQSEEKIVFFDRGIPDLYGYSKAF
                     CGEASKKVNEAVRRFRYNKTAFIFPPWSEIYENDDERQQDYQEALQTYYAVKEGYFDC
                     GYVLLEVPKCPIKDRANFIFQM"
BASE COUNT          204 a          150 c           87 g          204 t
ORIGIN      
        1 catttgaaaa ataaaattag cccggtcttt aatagggcat ttgggaacct ctaataacac
       61 atacccacaa tcaaaatacc cctcttttac agcataataa gtttgcaacg cttcttgata
      121 atcctgttgg cgctcatcgt cattttcata aatttcagac cagggaggga aaataaaggc
      181 tgttttatta taacgaaatc gcctaacagc ctcattcact ttcttactcg cctcaccaca
      241 aaatgctttg gagtaaccat acaaatccgg gattccccga tcgaaaaata caattttttc
      301 ctctgactgc atcttgcaat aatctgcaat cgattgttca agcatcaagt ctcgaaacgc
      361 ttttctattt ccagtatgta tcgcatcacc accatcagcc tgttgttttt gaataataga
      421 acgtgctact tcagaaactg ttaaatagcc ccaagtattt aactcattca atacggcagt
      481 tttgccagag ccaggtcctc ccgtaaatat aaaaaaatta gtgcatagca ttaaatcctc
      541 taccataacc tgtcatacct ttaaaaattg ctcataaatt aaattacaaa gcttttagtg
      601 tactcatatc aattctctta agcttttatc cagcttatct gtttc
//
LOCUS       DAXLDT010000399          609 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.401, whole genome shotgun sequence.
ACCESSION   DAXLDT010000399 DAXLDT010000000
VERSION     DAXLDT010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.401"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>609
                     /locus_tag="ACF2SP_003287"
     CDS             <1..>609
                     /locus_tag="ACF2SP_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFD2361024.1"
                     /translation="DQLEPVKKFLKGETLLQLGDCATNPWLNLLDFKHKWIASPFSST
                     NRIHLECSLNQLPLNRDTMDCVIVPLTLEPFGNNFSLIDEIDRILKPMGFLIFLCINP
                     WSLWGGAMKWGLLNCYGNQTVKLRSAFNLNRIFLQRGYKQCSLTNFCYIPPVNSQSLI
                     KKLTFFDEIGKMIWPFPSGFYCYITQKYEYISPSLTVEPAIES"
BASE COUNT          201 a          107 c          102 g          199 t
ORIGIN      
        1 agatcaatta gaaccagtaa aaaaattctt aaaaggagag acgttactac aacttggaga
       61 ttgtgctaca aatccctggt taaaccttct tgattttaaa cacaaatgga tagcatcacc
      121 attttcttct acaaatagaa tccatcttga gtgttcgcta aatcagttac cactgaatag
      181 agatactatg gattgtgtta tagtaccctt aacactagag ccgtttggaa acaattttag
      241 cttaattgat gaaattgacc gaatattgaa gcctatggga tttctcatat tcttatgcat
      301 taacccttgg agtttatggg gaggggctat gaaatggggg ttacttaatt gttatggtaa
      361 tcaaacggta aaattgcgtt cagctttcaa tcttaacaga attttccttc aaagaggcta
      421 caaacagtgt tctttaacta atttttgcta tataccacca gtaaatagcc aatctttaat
      481 taaaaagtta actttttttg atgagattgg caaaatgata tggccttttc catctggctt
      541 ttattgctat atcactcaaa aatatgagta cataagccca tcactcacag tcgaacctgc
      601 catagaatc
//
LOCUS       DAXLDT010000400          608 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.402, whole genome shotgun sequence.
ACCESSION   DAXLDT010000400 DAXLDT010000000
VERSION     DAXLDT010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.402"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..596)
                     /locus_tag="ACF2SP_003288"
     CDS             complement(<1..596)
                     /locus_tag="ACF2SP_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361025.1"
                     /translation="MVTKIIWVSNNGKPNLKIEFVSEEEKSNFFKEVKKKASELGLDF
                     PLVQGPGNSLVVQASNYPINPCGCYISPGGKLAINFGKVELSHFILPKVGVKTEHAEI
                     FKDHNTIFFHKHKLPGVNSELTFIPTGTPVIVPITKPATTSPTFFNPSKSGDSSISIS
                     AIGNVDSPMIRITFQNQTEREFFLSKITDKAKSLGVNIS"
BASE COUNT          186 a          117 c           96 g          209 t
ORIGIN      
        1 gagatattga ctcctaaact ttttgcttta tctgttattt tacttagaaa aaactctctc
       61 tccgtttgat tttgaaaagt aattcttatc atcggactat caacgtttcc tatcgcacta
      121 atagaaatcg aagaatcccc tgactttgaa ggattaaaaa atgttggaga agtagtcgcc
      181 ggttttgtta tgggaacaat aacaggtgta ccagtcggta tgaacgttaa ttcagaattc
      241 acacctggaa gcttatgttt atggaagaaa atagtattgt ggtccttgaa aatctcggca
      301 tgttctgttt tcacgcctac tttaggtaat ataaaatgac tcaactccac ttttccaaaa
      361 ttaatagcta atttcccccc tggggatata taacaaccac aaggattaat tgggtaattt
      421 gaagcctgca ccacaagaga atttccaggg ccttgcacta gtggaaagtc taatcctaat
      481 tcgcttgctt ttttctttac ctccttgaaa aaattacttt tttcttcctc actaacaaat
      541 tcaattttta aattaggttt accattatta gaaacccaaa ttattttcgt aaccataatt
      601 ggcctctg
//
LOCUS       DAXLDT010000401          608 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.403, whole genome shotgun sequence.
ACCESSION   DAXLDT010000401 DAXLDT010000000
VERSION     DAXLDT010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.403"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            24..>608
                     /locus_tag="ACF2SP_003289"
     CDS             24..>608
                     /locus_tag="ACF2SP_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361026.1"
                     /translation="MQQPTYSQTTKPPTGKKVIICFNSEEENEIQAFIRRFRVIHEEK
                     GFDPDDLDIQVIYPCDIASDQYMTWSGTEPTQEQKEILSKLKPQDKIYIWGHGAPNYA
                     YIPGAFYTEIAEYLEKGINKENFGPGKGPLNITVEICNGARGGSQGKDSFAAKLHSLL
                     GKKGIDSTVTGRLRNVFIDLQFLPLYGIKTIERAN"
BASE COUNT          231 a          106 c          115 g          156 t
ORIGIN      
        1 gcttacaata tcaggagacc atcatgcaac aaccaactta tagtcaaaca acaaaaccac
       61 ctaccggaaa aaaagttata atctgtttta acagcgagga agagaacgaa attcaagcct
      121 ttattagaag gtttagagta attcatgaag aaaaagggtt tgatcccgat gaccttgata
      181 tccaggtaat ctacccttgt gatattgcat cagaccaata catgacctgg tcaggaacag
      241 agcctactca agaacaaaaa gaaatcctaa gcaaacttaa accgcaagat aaaatttata
      301 tctggggaca tggcgctcct aattatgcct atattccagg agctttttac actgaaatag
      361 ctgaatatct tgaaaaaggt atcaataaag aaaattttgg gccaggtaaa gggccactta
      421 atattaccgt ggaaatatgt aatggggcac gaggaggaag ccaaggcaaa gatagttttg
      481 cagctaaatt acattcactt ttaggaaaaa aaggaattga tagcactgta acaggaagat
      541 taaggaatgt atttatagat cttcaatttt tacccctcta tggaattaaa actattgaac
      601 gtgctaat
//
LOCUS       DAXLDT010000402          608 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.404, whole genome shotgun sequence.
ACCESSION   DAXLDT010000402 DAXLDT010000000
VERSION     DAXLDT010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.404"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            3..566
                     /locus_tag="ACF2SP_003290"
     CDS             3..566
                     /locus_tag="ACF2SP_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361027.1"
                     /translation="MLKPQDIVILLKMLANKNPQQFIQKDLATYLCMSASEVHEGMKR
                     LTLSHLLVPVSQESDTNSKRKKILTPIKAACEECLIYGVKYFCPVRLGEYTRGIPTSY
                     AAPLFAKYIVLGSDPIPIWPYAEGDHRGLALEPLYRSVPQSITKHPDQIFYELLVFID
                     AIRTGRARERALAIKLLRERISLNEST"
BASE COUNT          191 a          120 c          118 g          179 t
ORIGIN      
        1 tgatgctgaa gccacaagac atagttatac ttttaaaaat gttagcaaac aaaaatcctc
       61 aacaatttat tcaaaaggat ttggcgacat atctatgcat gagcgcctct gaagtacatg
      121 agggcatgaa acgattaacg ctatcccatt tacttgtgcc agtgtcacaa gaatcagata
      181 caaacagtaa acgcaaaaaa atacttacac caatcaaagc agcttgtgaa gaatgcttaa
      241 tttatggggt aaaatatttc tgtcctgttc ggcttggaga atatactcgg ggtattccca
      301 caagttatgc agccccactg tttgcaaaat atattgtgct cggtagtgac ccaataccaa
      361 tatggccata tgctgaagga gatcatcgtg gtttggcttt ggagccattg taccgctcag
      421 ttccccaatc aattacaaaa catcctgatc aaatctttta tgaattgctt gtatttattg
      481 atgcgatacg tactggtcga gcaagagaac gtgctctcgc tattaaactt ttgcgagaaa
      541 gaatatcgct taatgaaagc acctagaggg tcagatcttg tcctttgcct cttgtatata
      601 atgttgtt
//
LOCUS       DAXLDT010000403          603 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.405, whole genome shotgun sequence.
ACCESSION   DAXLDT010000403 DAXLDT010000000
VERSION     DAXLDT010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.405"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(147..>603)
                     /locus_tag="ACF2SP_003291"
     CDS             complement(147..>603)
                     /locus_tag="ACF2SP_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361028.1"
                     /translation="KLSSKRSIINHDHLITDEACMIGIYREGGDLSIYAESSEGIEHF
                     SINRGNSLDMVILPHTLVGTWGHHQLLRTVEQAAQIRALRIRSHDYIEQSVTRKELEQ
                     FKEVFMLACKEEISNQPEIEDYLQTALGKLHGEPEMTYKPTFTEQCSIL"
BASE COUNT          168 a          112 c          107 g          216 t
ORIGIN      
        1 tctattttta acggcttttt gtttgaggtt agttgagaac gatacagttt gcaaataagc
       61 ctggtactaa tcgaactgat gaaggatatt aaaaaagctc gaagattttg aatctcgagc
      121 tttttctctg attattctaa taaaaattag agaatgctgc attgctctgt gaacgttggt
      181 ttgtaagtca tctctggttc cccatgtaac ttacccaaag cggtttggag ataatcttca
      241 atttcaggtt gattgcttat ttcctctttg catgcgagca taaatacctc tttgaattgc
      301 tcaagttcct ttcgggtgac tgattgttcg atgtaatcat gagatcttat tcttaatgct
      361 cgaatctgtg ctgcttgctc caccgtacga agtaattgat gatgccccca agttccaact
      421 aaagtatgtg gtaatatcac catatcaaga ctgttgcccc gatttataga gaaatgttca
      481 attccttcag atgattcagc ataaattgat aaatcacccc cttctctata aattcctatc
      541 atacaggctt catcagttat taaatgatca tgattgatga ttgaacgttt actgcttaat
      601 ttt
//
LOCUS       DAXLDT010000404          588 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.406, whole genome shotgun sequence.
ACCESSION   DAXLDT010000404 DAXLDT010000000
VERSION     DAXLDT010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.406"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..245)
                     /locus_tag="ACF2SP_003292"
     CDS             complement(<1..245)
                     /locus_tag="ACF2SP_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HFD2361029.1"
                     /translation="MYLFILKPKSLRFFNLVFITLALSFCLLANARAAGIERYDFGDM
                     ASKVTKAWDQDVALYVAKRFAIENSFDEREVTVKKLNA"
BASE COUNT          196 a          106 c          104 g          182 t
ORIGIN      
        1 gcatttaatt ttttgactgt tacctctctt tcatcaaaac tgttttctat cgcgaagcgc
       61 ttggcaacat aaagcgcaac atcttgatcc caagctttag tcacctttga ggccatatcc
      121 ccaaagtcat aacgttcaat tcctgctgct ctggcattag ctaataaaca gaaagacagg
      181 gctaaagtaa taaaaacaag gttaaaaaaa cgcaaggatt tgggtttaag gatgaatagg
      241 tacaatttaa ctggtatatg aattaaaatt gagcatattg tatatcgttt gcattcgcaa
      301 atcaaataat tagaaaagaa aaattgttgt tctcactata tccagaaact aagaaaatca
      361 ataaaagcgc ctttaaggct ctaaaggctc atgggaaaac tctggattcc tggaaaggag
      421 aaatacatgc gtataattat gttacaactt cccctgggac ttgttcccag ggcacagagc
      481 ctctttggag gctcttgttt tttagatatg cactattttt attgaacatc atctttcttg
      541 gtatccgact tgataaacaa acttattggt aaggcgaata atttaatt
//
LOCUS       DAXLDT010000405          587 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.407, whole genome shotgun sequence.
ACCESSION   DAXLDT010000405 DAXLDT010000000
VERSION     DAXLDT010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.407"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>587
                     /locus_tag="ACF2SP_003293"
     CDS             <1..>587
                     /locus_tag="ACF2SP_003293"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD2361030.1"
                     /translation="DKGKEKVNYIDEYSNTKIKRAFHYLTDEEKEQVITAIGKYAQAL
                     EQSRLIRDQIRILRLSTSRALRKQIVNMIENIQVNEFHLSITPEINASILKAEEEYCY
                     YSSCNFWYAVDEQGGIIGSIGLKKLNHTEGEVKKFFIAPKYRGLGLAQKLMLTLVRSA
                     MKHGFKKLFLGTVAQLNAARRFYEKNGFVLVNKNS"
BASE COUNT          207 a           69 c          124 g          187 t
ORIGIN      
        1 gataagggta aggaaaaggt aaattatatt gatgaatatt ctaatactaa aataaaaaga
       61 gcatttcatt atttaactga cgaagagaag gaacaagtaa ttactgcaat tggaaagtat
      121 gctcaagctt tagaacaaag tcgtttaatt cgtgatcaga ttagaatttt acgcttgtca
      181 acttccagag ctttgcgtaa acaaattgtt aatatgattg aaaatattca agtgaatgag
      241 tttcatcttt cgattactcc tgaaattaat gcatcaattt taaaggccga agaggagtat
      301 tgctattata gcagttgtaa tttttggtat gcggttgacg aacagggggg gattatcggt
      361 tctatcggtt taaagaagtt aaatcatact gaaggggaag taaaaaaatt ttttattgcg
      421 ccgaagtatc gagggttagg attagctcaa aaattaatgc tgactttagt tagaagcgcc
      481 atgaaacatg gatttaaaaa gctgtttttg ggaacagtag cccagttaaa cgcagcaaga
      541 cgtttttatg aaaagaatgg atttgttctt gttaataaga attcttt
//
LOCUS       DAXLDT010000406          582 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.408, whole genome shotgun sequence.
ACCESSION   DAXLDT010000406 DAXLDT010000000
VERSION     DAXLDT010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.408"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            122..>582
                     /locus_tag="ACF2SP_003294"
     CDS             122..>582
                     /locus_tag="ACF2SP_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HFD2361031.1"
                     /translation="MKKSIKRSSDTLLEIASHQELKGEVTYQRILQVLGERAFGIVLL
                     FFALPSALPFSFIPGISLIFSVPILLFAFQMVFARKTMWLPKIIAERTIHQETIAKFI
                     HNTVPYLIKAEYFLKPRWLFMTSRFMEIIHGIVIFFLALLLMLPIPFSNFI"
BASE COUNT          167 a           99 c           98 g          218 t
ORIGIN      
        1 ttgaagtacc tctgtgtttt ctgcagagtt agagaaaata tgagctttaa aaattcttaa
       61 ggtaaaaatt ttcctcaaac agttggtaaa ttaatgttat atattttgac ttggattttt
      121 tatgaaaaaa agcattaagc gctcttctga taccctttta gaaatcgcaa gtcaccaaga
      181 gttaaagggg gaagtcactt atcaacgtat tttgcaagtg cttggagagc gtgcctttgg
      241 aattgtgctt ttattttttg cgttgcctag tgcgctgcct ttctcattta ttcctggtat
      301 ctccctgatt tttagtgttc cgatattact atttgcattt caaatggttt ttgccagaaa
      361 aacaatgtgg ctaccaaaaa ttattgcgga acggaccatt caccaggaaa caatagccaa
      421 atttatacat aataccgttc cttacttaat taaagctgaa tactttttaa aacccagatg
      481 gctgtttatg acttctcgct ttatggaaat aatccatgga attgttattt tcttccttgc
      541 actactgttg atgttaccaa ttccatttag taattttatt tt
//
LOCUS       DAXLDT010000407          567 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.409, whole genome shotgun sequence.
ACCESSION   DAXLDT010000407 DAXLDT010000000
VERSION     DAXLDT010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.409"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>567)
                     /locus_tag="ACF2SP_003295"
     CDS             complement(<1..>567)
                     /locus_tag="ACF2SP_003295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361032.1"
                     /translation="YLNMSNPVIDNVSSPESRGKRIRFIREHLLSFTREDFCRDSNFT
                     AQSLKGWELAWGGGLSQQGAVKIVKRSKELNIYCTESWLMHGIGKNATRITKDLDIQE
                     GDESHIAKELLLFRELQNSIDTVIKDDGMIPFLYPGNYVGGIIVNNIESAIGKDCIII
                     ADNDEVFVRILKHGEEPARYDLVCLNEK"
BASE COUNT          164 a          120 c           90 g          193 t
ORIGIN      
        1 ttttttcatt taaacaaact aagtcatagc gagctggttc ttctccatgc tttaagatcc
       61 taacgaatac ttcatcattg tccgcaataa taatacaatc tttgccaatg gcactctcaa
      121 tattgttgac tattataccg ccaacataat tgccagggta taagaaaggt atcatgccat
      181 catcttttat gacagtgtca atagagtttt gtagttctct gaatagtagt aattctttag
      241 ctatgtggct ttcgtcaccc tcttggatat ctaaatcttt tgtgattctt gtggcatttt
      301 taccaatgcc atgcattaac catgactctg tgcaataaat atttaactct ttagatcgct
      361 taacgatttt tacagccccc tgttgggata aaccccctcc ccaagccaat tcccatccct
      421 ttaatgattg agcagtaaaa ttagaatcgc gacaaaaatc ttctcgtgta aaagacagaa
      481 gatgctcgcg tatgaatctt attcttttgc cccgagactc gggggatgaa acattgtcta
      541 ttacaggatt gctcatattt aaataat
//
LOCUS       DAXLDT010000408          560 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.410, whole genome shotgun sequence.
ACCESSION   DAXLDT010000408 DAXLDT010000000
VERSION     DAXLDT010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.410"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..351)
                     /locus_tag="ACF2SP_003296"
     CDS             complement(<1..351)
                     /locus_tag="ACF2SP_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361033.1"
                     /translation="MEHSWYDNNSKLSVMGIICLIIFLVLFFFSLFIMPFLIWELHYN
                     VPDFVSNMIAYLEDTYYYSSPVSKILVWLTFFIPCLITGYFSYYISPSIDKKNLLKDA
                     NIDEEQSKEISSVTN"
BASE COUNT          215 a          115 c           70 g          160 t
ORIGIN      
        1 attagttacc gaagaaattt cttttgattg ttcttcatca atgtttgcgt ctttcagcaa
       61 atttttctta tcaatagagg gagaaatgta ataagaaaaa tacccagtaa tcaagcaagg
      121 tataaaaaaa gtcaaccaga caagaatctt acttacaggc gaagaataat aataggtatc
      181 ttcaaggtat gctatcatat tgctaacaaa gtcaggaaca ttataatgta attcccagat
      241 taaaaaaggc attataaaca gactaaagaa aaagaggact aaaaaaataa tcaaacaaat
      301 tattcccatt acagataatt tgctgttgtt atcataccaa ctatgctcca taggctcctt
      361 ccataaaatg accagctaac taaaattatt cttttttaaa ttttaagaca tcagcagcat
      421 ccatcatata accgaatata ttttaagcta gatattaaaa aatagcttaa cgacaaataa
      481 acttatttcc ggccgcgtct aacgtcctcc aacaccacca cccccacgac cacctccata
      541 tcccccatgc cctttatgct
//
LOCUS       DAXLDT010000409          555 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.411, whole genome shotgun sequence.
ACCESSION   DAXLDT010000409 DAXLDT010000000
VERSION     DAXLDT010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.411"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            167..>555
                     /locus_tag="ACF2SP_003297"
     CDS             167..>555
                     /locus_tag="ACF2SP_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD2361034.1"
                     /translation="MKTKQEVSQQDKLKDSKSSTPLQTKETWFISDTLNIIFDSYDFS
                     ISVIEQALMPYRIVFSGGGSRILAHIGAMDELTRHGLQFTEFSGSSAGAMVAAFAYLG
                     YNCSEIKQIISWFNEDKLLDSPLIFNF"
BASE COUNT          193 a           97 c           93 g          172 t
ORIGIN      
        1 ttaaaatacc caataactga aacatcaata atttaaaaaa ggagagaact ctcaatacaa
       61 aaaccttgtg acaaaatcgt tttaaatact cgatagattg tgatggctgt agcaaaagaa
      121 tcgtaaaaaa taacttagaa tttgttttat ataagggatt atcggtatga aaacaaagca
      181 agaagtttct cagcaggaca aattaaaaga ttccaaatcg tctacacctc tacagacaaa
      241 agaaacatgg ttcatctctg ataccttaaa tattattttt gactcctatg attttagtat
      301 ctctgtcatt gaacaagctc tcatgccata tcgtattgtt ttcagtggcg gaggctcaag
      361 aatattggca catataggtg ccatggatga gttaaccagg catggattac agttcactga
      421 attttccgga agctcagccg gtgcaatggt cgctgccttt gcttatcttg gttataattg
      481 ttccgaaata aaacaaatta tttcctggtt taatgaagat aaactccttg atagcccact
      541 catttttaat ttcaa
//
LOCUS       DAXLDT010000410          550 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.412, whole genome shotgun sequence.
ACCESSION   DAXLDT010000410 DAXLDT010000000
VERSION     DAXLDT010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.412"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..418)
                     /locus_tag="ACF2SP_003298"
     CDS             complement(<1..418)
                     /locus_tag="ACF2SP_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD2361035.1"
                     /translation="MFERKLTPIITELLAEFRIVYLTGPRQAGKTTLTKIIAQNSGLE
                     YISFDNQTILQSAQNDPIGFINSLHNKKVVLDEFQYIPELIPAIKQASDNLPPNVKGK
                     FLLTGSADIFRSGKTQEALPGHMARLELYPLSLSEQY"
BASE COUNT          177 a           87 c           94 g          192 t
ORIGIN      
        1 tgtattgttc tgataatgat agtggatata gttctaatct cgccatatgt ccaggtaaag
       61 cttcttgtgt tttccctgat cgaaaaatat ctgctgatcc ggttaatagg aattttcctt
      121 tcacattagg tggcaggtta tctgatgctt gctttatggc cggaattaat tcaggtatat
      181 attgaaactc atccaaaacg acctttttat tatgcaaaga atttataaaa ccaatcggat
      241 cgttttgagc tgattggaga attgtttgat tatcaaagga aatatattca aggccactat
      301 tttgggcaat tatttttgtt aaagttgttt tgcctgcttg ccgtggtccg gttaaataaa
      361 caattctgaa ttcagctagt aactcagtga taataggggt taattttctt tcaaacatcg
      421 ggattcatat aataggtaac atattttcaa tatataacac ctcttaaaaa atagctaata
      481 aaaacccgaa ttatccagta taaaaatccg aattatccaa tattataacc cgagaaatcc
      541 aaggtgatat
//
LOCUS       DAXLDT010000411          549 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.413, whole genome shotgun sequence.
ACCESSION   DAXLDT010000411 DAXLDT010000000
VERSION     DAXLDT010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.413"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            187..>549
                     /locus_tag="ACF2SP_003299"
     CDS             187..>549
                     /locus_tag="ACF2SP_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HFD2361036.1"
                     /translation="MKFRFRFYLGIILLILAIVCPLLIPLIVQSNFSVLIKGFLSSIL
                     VFGLPEVLIVLAIALLGKEVYGLLEQKIKNILFKEKVSRARYRLGLVFFAFPLIIGLL
                     EVFVKEITLAYGSYYYWVE"
BASE COUNT          151 a           71 c          119 g          208 t
ORIGIN      
        1 taaataggat aaaagagttg aaaaagtcat agtgttggat ttcatagaat ctggtggtta
       61 tagggaaaaa gcgggtgagc gatgctttgc tttaataatt gcaggaatta atggttcatt
      121 ttgtagatat tcaatcttct tctggattac tattgataag taaggtaaat agtgtgagta
      181 gctatcttga aatttcggtt tcgtttttat ctgggtatta ttcttttaat tttggcgata
      241 gtttgccctt tgctgatccc gttaattgtg caatccaact tctctgtgct gataaaggga
      301 tttctatctt caatattagt atttggtctt ccggaagttt tgattgtctt agctatcgct
      361 ttacttggaa aagaagtgta tggccttctc gagcaaaaaa ttaaaaatat cctttttaag
      421 gaaaaagtat caagagcccg ttaccgcctg ggtttggtat tctttgcttt tccacttatt
      481 atagggttgc tggaggtgtt tgtaaaagaa attaccttag cctatggaag ttattattat
      541 tgggtagaa
//
LOCUS       DAXLDT010000412          546 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.414, whole genome shotgun sequence.
ACCESSION   DAXLDT010000412 DAXLDT010000000
VERSION     DAXLDT010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.414"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>546
                     /locus_tag="ACF2SP_003300"
     CDS             <1..>546
                     /locus_tag="ACF2SP_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD2361037.1"
                     /translation="NLTIGTSKFNPPFEVWSGNNSSLHGFDIDLMQEICRRLHATCAF
                     EAYIFDDLFPALKNRKVDLVIASMIITDERKKLFIFSLPYMESNSQYITTIHSKINTF
                     DDLQDKKIGVRKGTPYARQVLSANRNNQVIFYELIQDMLLGLSNNEVDASLMDYEAAK
                     YWMASEPYAYKLIGNKISIGEG"
BASE COUNT          191 a           86 c           95 g          174 t
ORIGIN      
        1 aaatttaaca attggtactt caaaatttaa tccacctttt gaagtttggt ctggcaacaa
       61 cagttcattg catggatttg atatagatct tatgcaagaa atatgtagaa gattacatgc
      121 gacctgtgct tttgaagcct acatctttga tgatttattt cctgcattaa aaaaccgtaa
      181 ggtcgatctg gtaattgctt caatgattat tacagatgaa agaaagaaac tctttatttt
      241 tagtcttcct tatatggaaa gcaactcaca atatatcact acgatccatt ctaaaatcaa
      301 tacatttgat gatctgcagg acaaaaaaat tggagtaaga aaaggaaccc cttatgcaag
      361 gcaagttttg tcagcaaacc gcaacaacca ggtaattttt tatgaactta tacaagatat
      421 gcttcttggc ttaagcaata atgaagttga tgccagttta atggattatg aggcagcaaa
      481 atattggatg gcatctgaac cctatgctta taaattaatt ggtaacaaaa tttctattgg
      541 agaagg
//
LOCUS       DAXLDT010000413          546 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.415, whole genome shotgun sequence.
ACCESSION   DAXLDT010000413 DAXLDT010000000
VERSION     DAXLDT010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.415"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>546
                     /gene="lem11"
                     /locus_tag="ACF2SP_003301"
     CDS             <1..>546
                     /gene="lem11"
                     /locus_tag="ACF2SP_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem11"
                     /protein_id="HFD2361038.1"
                     /translation="LSDKTILQELKIEGELAAIRNDYLKKKRTNPEVTPFIDQKDVAN
                     PFEIYKKIGELAKIDLLKDLENRTLAYLNFSVKIQTEKIAGMLNDLLNRVQNQQVIVS
                     ELYQYLLPYKGKDDLFDALIFSLKRELKKLSHNFSPKTQLSSISKINLVLVPSLKLDH
                     LPRHNPEIVNICEKLENYVFRG"
BASE COUNT          219 a           89 c           83 g          155 t
ORIGIN      
        1 ttatcagata aaaccatttt acaagaatta aaaattgaag gagaattagc tgcgattaga
       61 aacgattatt taaagaaaaa aagaacaaac ccggaggtca ctccatttat tgatcaaaaa
      121 gatgtagcaa atcctttcga aatatacaaa aagataggtg aattagccaa gatagatcta
      181 ctaaaggatt tggaaaaccg tacacttgcc tatctgaatt tctcagtaaa aatccagaca
      241 gaaaaaatag ccggcatgct aaatgattta ttaaatcgtg tacaaaatca gcaagttata
      301 gtttctgaat tgtaccaata tcttctacct tataaaggca aagatgattt gttcgatgcc
      361 ttgatatttt cacttaaaag agaattaaaa aaattaagtc ataatttctc tcctaaaaca
      421 caactttcca gcatatcaaa gataaatctc gtgcttgttc caagtttaaa acttgaccac
      481 ctgcccaggc acaatccaga aatagtgaat atttgtgaaa aattggaaaa ctatgtcttt
      541 agagga
//
LOCUS       DAXLDT010000414          524 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.416, whole genome shotgun sequence.
ACCESSION   DAXLDT010000414 DAXLDT010000000
VERSION     DAXLDT010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.416"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(37..>524)
                     /locus_tag="ACF2SP_003302"
     CDS             complement(37..>524)
                     /locus_tag="ACF2SP_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361039.1"
                     /translation="PNRLSYDFQNYEDMTKKWISTLQLSDYNVLYSVHPSGFPDTANA
                     IRSLDQIVVEEDIANLIPLCDVYLASVSSTIKWARACNKLVLDYDCFHFNYRTYEKDP
                     AIIAVKSLNELKEWITRLNNQSVSKIHDQLNPSLHPNYWGIIDGRSLWRINNYIDCLI
                     K"
BASE COUNT          188 a           90 c           78 g          168 t
ORIGIN      
        1 gttacaattt ttttaaacat tcatagagta tcaaagttat ttaatcaggc aatcaatata
       61 attattaatt cgccataaag acctcccatc aataattccc caatagtttg gatgtagaga
      121 aggatttaat tgatcatgta tctttgatac tgattgatta ttgaggcgag taatccattc
      181 tttcagctca ttcagacttt taacagcaat aattgcagga tccttttcat aagtgcgata
      241 attaaaatga aaacaatcat aatccaaaac aagcttatta caagctcttg cccatttaat
      301 agtacttgaa acagaagcca gataaacatc acaaagagga atcaaatttg ctatgtcttc
      361 ttccacaaca atctgatcca gagagcgaat tgcatttgct gtgtcaggaa acccacttgg
      421 gtgtacagaa tataaaacat tgtaatcaga taattgaagc gtagatatcc atttcttagt
      481 catatcttca tagttttgaa aatcatatga taaccgattt ggaa
//
LOCUS       DAXLDT010000415          511 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.417, whole genome shotgun sequence.
ACCESSION   DAXLDT010000415 DAXLDT010000000
VERSION     DAXLDT010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.417"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            63..>511
                     /locus_tag="ACF2SP_003303"
     CDS             63..>511
                     /locus_tag="ACF2SP_003303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361040.1"
                     /translation="MSQEKTGESEQLVKKKEQLEMDVRNLKQKTQEHKKVIDELSQQI
                     VALNNQIVELKLKNEEQTHRISGLEAEKIQEKQRSQTAENNAQAELIQQLLSPKEISC
                     ANLIEAQLVPSTNDYLHHLIEQAKKINPLVTDNIYEKLPPFNGSEAD"
BASE COUNT          225 a           79 c           82 g          125 t
ORIGIN      
        1 ttattgagca attaagagaa agggaaaagg agttatattc agtaaataat gaattacgcc
       61 ttatgagtca ggagaaaact ggggaatcag aacaattagt caagaaaaaa gaacaattag
      121 aaatggatgt tcgaaattta aaacagaaaa cccaagaaca caaaaaagta attgatgaat
      181 tatctcaaca aatagtagct ctaaataatc aaatcgtaga gctaaaactt aaaaatgaag
      241 aacagactca tagaattagc ggattggaag ctgaaaaaat tcaagaaaaa caaagatcac
      301 aaactgctga aaataatgcg caagctgagt taattcaaca actgctttca cctaaagaaa
      361 ttagttgtgc taatcttatt gaagcccaat tagtaccatc aaccaatgat tatcttcacc
      421 atttaatcga acaagcaaaa aaaattaatc ctttggtcac tgataatatc tatgaaaaac
      481 tgcctccatt caatggctct gaagctgata a
//
LOCUS       DAXLDT010000416          503 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.419, whole genome shotgun sequence.
ACCESSION   DAXLDT010000416 DAXLDT010000000
VERSION     DAXLDT010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.419"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>503
                     /locus_tag="ACF2SP_003304"
     CDS             <1..>503
                     /locus_tag="ACF2SP_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361041.1"
                     /translation="AKARIKYELKQLETQLGKCVKQPDDTAFSKNKYSLYMALADKYS
                     EYLKLIDINSKFKQFVGAEIPKDISYWEACTRIMHLKQKRSAAVNLLEEAKIIIEISL
                     LYQCLAKAIEPNEVSDVSNHLILNQFDKLKQLFKKENLELWLSTLVDLWNHNSTLLRE
                     PAKLFRN"
BASE COUNT          169 a           76 c          106 g          152 t
ORIGIN      
        1 gccaaggcgc gaatcaaata tgagttgaaa cagttggaga ctcagcttgg caaatgcgtt
       61 aagcagccgg atgatactgc cttttcaaag aacaagtact cactttacat ggctttagcg
      121 gataaatatt cggagtattt gaagttaata gatattaatt ctaaatttaa acaattcgta
      181 ggggcggaga tacctaagga tattagttat tgggaagctt gcactcggat tatgcatctg
      241 aaacagaaaa ggtctgcagc agtgaatttg ctggaggaag ctaaaattat cattgagata
      301 tcattattgt atcaatgtct tgctaaagca attgaaccaa atgaggtttc agacgtaagc
      361 aatcatttaa tcctgaatca gtttgataaa ctaaagcagt tatttaagaa agagaatctt
      421 gaattatggt tatcaactct ggttgatctt tggaatcaca attctacttt attaagggag
      481 ccagccaagt tatttcgcaa ttg
//
LOCUS       DAXLDT010000417          502 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.420, whole genome shotgun sequence.
ACCESSION   DAXLDT010000417 DAXLDT010000000
VERSION     DAXLDT010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.420"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>502
                     /locus_tag="ACF2SP_003305"
     CDS             <1..>502
                     /locus_tag="ACF2SP_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD2361042.1"
                     /translation="ELGIYTSLFIDKISPEIHLHQSEILGNVTFDSKMGAEIGSKINA
                     ITPGILLNNNPLKEAYVDLNNTLKEINTLLDEENKKARDNPCRNEKIAKLTSLKDRLS
                     DLDSIPKENTVEFLKKMQEETRSSLKSLESNDALINIYDVLNSLKETIENGSDLPEIK
                     KDKLQMI"
BASE COUNT          195 a           71 c           96 g          140 t
ORIGIN      
        1 gaattgggaa tttataccag tttatttatc gataaaataa gcccggaaat tcatttgcat
       61 caatctgaaa tattgggaaa tgttacgttt gacagcaaaa tgggggctga aattggctca
      121 aaaattaatg cgattactcc cggaattttg ctgaataaca atccattgaa agaggcgtac
      181 gttgatttaa ataatacatt gaaggaaatt aatacattac ttgatgagga aaataaaaag
      241 gccagggata atccttgtcg aaatgaaaaa atagccaaat tgacttctct aaaggatcgt
      301 ttgagtgatt tggacagtat tcctaaagaa aatacagtgg aattccttaa gaagatgcaa
      361 gaagaaacac ggtcttcgct aaaatctctg gaaagcaatg atgccttaat caatatttat
      421 gatgtactta attctttaaa agaaaccata gagaacggga gtgacttacc cgaaatcaaa
      481 aaagacaagt tacaaatgat tt
//
LOCUS       DAXLDT010000418          489 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.421, whole genome shotgun sequence.
ACCESSION   DAXLDT010000418 DAXLDT010000000
VERSION     DAXLDT010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.421"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          187 a           71 c           77 g          154 t
ORIGIN      
        1 ttaaatagct tattattgcc ttacaaagaa ggactatact atttaggatt agcaaataaa
       61 gaaattaata atccttcact agattgaata ttagatttaa tggaacagtt gtctatttta
      121 aaccgattta actttaattc aactactatt caactaatgc taagaaaaag aaccattcat
      181 tattcagaag catttacacg ttttaatccg ggtgatttaa tacaaatgac tgataacaag
      241 cggtgcgctc atttaataat atattgtcac acccgaaaag aaaacgttat agataaaacg
      301 attgatatgt ttaaccgcat catccgtaac gtgattcata aatcggaaaa gagcgtagtt
      361 agaaagctta tcaatgatgt aaaaaagtct acggcaaaga tactattcta tttaatatag
      421 ctgaagtatg cttggaacag ccggatgcaa taattcgaga taaaattttt catattgttg
      481 gtcaggata
//
LOCUS       DAXLDT010000419          485 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.422, whole genome shotgun sequence.
ACCESSION   DAXLDT010000419 DAXLDT010000000
VERSION     DAXLDT010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.422"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          172 a           99 c           73 g          141 t
ORIGIN      
        1 tacatccaat gccttcgatt aaacaacatg cgcaatataa aattaaattc gtctaatttc
       61 aataatattc gccggtgctg attaattgat tgataatttc tctcataaaa aacaaaatgg
      121 aaccatcgtg agatttgcgt atttgattac ggcacagtac accataatat gccatttaca
      181 gtggttttca cgcagcgaat tttttaataa aaccaaggat ggctcataat ccgttggccc
      241 taggataaag tcctagtggg cccgccaata aaatcagggg ggctattcct aagacatcgg
      301 ttacaaatta tatcggaaac ataggtaaca ctttaggccc taaaagatta cataaacttc
      361 caagaagcag cggactaaaa aacgccactt ccccatataa gttgatcatc tacatcacca
      421 ttcatcctcc agaattttaa tactcattat taaaatttca ttaagaattt taagaatacg
      481 ctaat
//
LOCUS       DAXLDT010000420          459 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.423, whole genome shotgun sequence.
ACCESSION   DAXLDT010000420 DAXLDT010000000
VERSION     DAXLDT010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.423"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>459
                     /locus_tag="ACF2SP_003306"
     CDS             <1..>459
                     /locus_tag="ACF2SP_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFD2361043.1"
                     /translation="MTIPNYDWHGLIHRLISENNGGNLQVYIQVTRGNQGVRKHDIPT
                     SITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAVSA
                     GFHTSILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPGITRQVVIE"
BASE COUNT          149 a           88 c           90 g          132 t
ORIGIN      
        1 agatgactat accaaattat gattggcatg gtttaattca tagactaata tcagaaaata
       61 atggaggtaa tttacaagta tatatccaag tcacacgagg aaatcaaggg gtgcgcaagc
      121 atgatatccc tacttccatc acaccttctg ttatcgcatt caccatgcat aattcatttc
      181 ccaccctcga agataaggaa cagggaatgt cagcaaaact ggtcgaagat tttcggtgga
      241 tgagatgtga tataaaaact acttctttaa ttgccaatat attactgaat gatgaggctg
      301 tatctgcagg attccacact tcaattcttg cccggaatgg tctaattaca gagggaagta
      361 gtaccaacgt attcattgtc gcacaggatg gtgttattaa gaccccaccc atgaataatt
      421 tctgtttacc aggaattact cggcaagttg tgattgaaa
//
LOCUS       DAXLDT010000421          442 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.denovo.424, whole genome shotgun sequence.
ACCESSION   DAXLDT010000421 DAXLDT010000000
VERSION     DAXLDT010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.denovo.424"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>442
                     /gene="lem4"
                     /locus_tag="ACF2SP_003307"
     CDS             <1..>442
                     /gene="lem4"
                     /locus_tag="ACF2SP_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem4/SmdA"
                     /protein_id="HFD2361044.1"
                     /translation="TIGPGEDKYKAVVSHEKKPECLPGFRLICDDDEIDGEEMYNGLK
                     HTGRSQLVKYHNEKPGQFKWGEYFKEILTNCDIAVKEYLNGKIGSRFHLFTAKVDEKT
                     GDQSFKDRYSQVKGDILKRAIINNLKNDIEKIDNLDDLKDFIKKF"
BASE COUNT          181 a           54 c           90 g          117 t
ORIGIN      
        1 tacaataggg ccaggggaag acaagtacaa agcagtggta tcacacgaaa aaaaacctga
       61 gtgtttacca ggatttcgtt tgatttgtga tgatgatgag atagatgggg aggagatgta
      121 taatgggctt aaacatactg gacgctccca acttgtaaaa taccataatg aaaagcccgg
      181 ccaatttaaa tggggtgaat attttaagga aatattaact aattgcgata ttgctgtaaa
      241 agaatacctg aacggaaaaa taggttcacg cttccatctt tttacagcaa aggtagatga
      301 aaaaacaggt gaccaatctt ttaaagatag atacagtcaa gtaaaaggag atatattaaa
      361 acgcgcaata attaataatt taaaaaatga tatagaaaaa atagataatt tagatgattt
      421 aaaggacttt ataaagaaat tt
//
LOCUS       DAXLDT010000422         5195 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.guided.198, whole genome shotgun sequence.
ACCESSION   DAXLDT010000422 DAXLDT010000000
VERSION     DAXLDT010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.guided.198"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..969
                     /locus_tag="ACF2SP_003308"
     CDS             <1..969
                     /locus_tag="ACF2SP_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HFD2361045.1"
                     /translation="EVKMEMLEGEKGVCSAFKNYKEYRSFMPKEELKTTEFGVLYEAN
                     EAFLEYFYNRSENTLINKDSPFEKYFVDSSSDEEAIKNYWDAEGYTLYCQHLAKPQVK
                     LSYIEIMTMMRVINQPLTIYDRSTSSVVAECVNPKVNLPDFEVAIDALQGHYFLLKTE
                     ETEKELEEYERSHAQYKRDRSEILAHSDKPVSSLLVRATCPKGHLDEDPFVALIESLS
                     EINSLSQIDTNLKNQNTDTPNCNFLLKVGASVVGASSALITLMGIAILSGQYDPGNSL
                     AEEALATTVVSSIVGLGYGLYSFFSSSPKVPELDSTNGLSQKLKMN"
     gene            1161..1580
                     /locus_tag="ACF2SP_003309"
     CDS             1161..1580
                     /locus_tag="ACF2SP_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFD2361046.1"
                     /translation="MSAKKNSIAHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFTPIVTKSDYENTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQTEVM"
     gene            1580..3490
                     /locus_tag="ACF2SP_003310"
     CDS             1580..3490
                     /locus_tag="ACF2SP_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFD2361047.1"
                     /translation="MLLIEIVLTIHCLLLLSSLFIGRAWLANKPSFKLKLLRFLLASC
                     IISPIIVHCVNSDQKPERQNYVAFDALQQYARQPIFKTESSKFNHGSSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLTEAIPYRTSGKLVIKISHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLASNREIACTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSAAGSLTSSMVKE
                     VDISHLDPRIQQIAEKQIMTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYFIAGKTFTNYSSNVESASLAEA
                     IAKSINVCLIRVSQEAGVPVIRKKLTEFGFDTSTWWQADRSDGLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETTNYSINHMLENAVTNGTGKLAVISGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVVLVVIEEGHFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSVNQ"
     gene            3540..4340
                     /gene="blaOXA"
                     /locus_tag="ACF2SP_003311"
     CDS             3540..4340
                     /gene="blaOXA"
                     /locus_tag="ACF2SP_003311"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HFD2361048.1"
                     /translation="MKKIIIFLYTGLLFCVSTWAQDTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENTTFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4400..>5195)
                     /locus_tag="ACF2SP_003312"
     CDS             complement(4400..>5195)
                     /locus_tag="ACF2SP_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFD2361049.1"
                     /translation="PNSPEKLLKITIANKTYTLSELKKQFKTIRVIISHNPAYNYQKK
                     TYDAFDMNQILEQLMGADLQKNNANQVLLAQTIDKYVSQTPLLYFTTKGHAYLAYQET
                     PGTISDNIITKDGRWSYINQHGKKENPGPFYIVWDNTSTYPVSWPYQVISMQIVNKKE
                     LTFSHFLNPLNESASVQNGHHIFNNICSTCHSLFYKGAQGRAPDLGMVMSYLTPSDIS
                     KLVKNGRGYMPPIGKNLSTEEINDLIKFLIWVNKQASKLKCEINNQ"
BASE COUNT         1628 a          909 c         1020 g         1638 t
ORIGIN      
        1 gaagttaaaa tggaaatgct ggaaggtgag aaaggagtat gcagtgcttt taaaaattat
       61 aaagagtatc gctcatttat gcccaaggaa gaattaaaaa caacagaatt tggtgtgtta
      121 tatgaagcga acgaggcttt tttagagtat ttttataatc gctctgaaaa tactttaata
      181 aataaggatt caccttttga aaaatatttt gttgactcgt cctcagatga agaggcaatt
      241 aaaaattatt gggatgcaga aggctataca ttatattgtc aacatttggc taaacctcag
      301 gttaaattat cttatattga aattatgacc atgatgaggg tcattaatca gccacttaca
      361 atatatgatc gttctacctc ctcagttgta gccgagtgtg tcaacccaaa ggtcaattta
      421 cctgattttg aagtggcaat tgatgctctt caagggcatt attttctttt aaaaactgag
      481 gagacggaaa aagagttaga agaatatgaa agaagtcatg cccagtataa aagagataga
      541 agtgaaatcc ttgcacactc cgataagcct gtttcttcct tattggttcg agctacttgc
      601 cccaaaggtc atttggatga agatcctttt gttgcattaa ttgaaagcct atccgaaata
      661 aacagcctta gccaaattga tactaatttg aaaaaccaga atacagatac ccccaattgc
      721 aacttccttt taaaggtagg tgcttctgta gttggtgcct cttctgcgct aataacttta
      781 atggggatag ccatattatc tggccaatat gatcccggaa acagtctggc tgaagaggca
      841 ttagctacta ctgttgtatc cagtattgtg ggtttaggtt atgggcttta tagcttcttt
      901 agctctagcc ctaaggtacc agagcttgat tctaccaatg gattatcgca aaaacttaaa
      961 atgaactaac ctcctgaatt agaaaatatt gtctgtaagc cgatcgtgtt gatattaggt
     1021 atcaacacga ttgcgccatt caagtaaact gttctcgcgt gtagagaagt atttttccca
     1081 tgactatttt aaccctacta tttttatagt agaatcctac tataaaaata gtagggcgta
     1141 tgtggttatt ggagagaaac atgtcggcaa aaaaaaattc gatagctcat gatcgacttc
     1201 ttacagaagt agagcttgaa ttgatgaata ttatctggag ccttgataag gtaactatta
     1261 aggaggttgt ttcacatctg cctaaggaaa ggcctttggc ttatacaacc gtggcaacag
     1321 tactaaaagt gctggaacaa aagggatttc ttgagtgtca aaaaaattcc tatgctcatg
     1381 tttttacacc catagttaca aagtcagatt atgaaaacac ttgcattgat catatggttg
     1441 ccaatgtttt tgatggagag ccccgagcac ttgtccaaag acttttgtta gccagaaagt
     1501 tacagcatga tgacattcaa gccattgaag aagcacttaa acaattaaac gctggtgaaa
     1561 aacagacaga ggtgatgtaa tgttattaat agaaatcgta ttgactattc attgcctgct
     1621 tcttctcagt tcactattta tagggcgcgc ctggctagca aataaaccat cttttaaatt
     1681 gaaattactc cgttttttat tggccagttg cattatttca ccaataatag ttcattgtgt
     1741 aaactctgat caaaagcctg aacgacaaaa ctatgtagca tttgatgctt tgcaacagta
     1801 tgcaaggcaa cccatattta agacagaatc ctcaaaattt aatcatggat cgtcttctgc
     1861 ctttacatca atgaatgtga actactgtca actcttaagt gtgatgtttt ttttaatcgt
     1921 cttgtttcgt agttatcact tgttatcagg tttaggtcgc ttgaaattga tgttgactga
     1981 agcaatccca tatcgaactt ccggaaaatt agttattaag atttctcacc actgccatgt
     2041 tcctttctca atattgctat ttaataaagc ctacatcgta ttgccagtat ccatattttc
     2101 ttcttccatg aatgtgagga ttgctattgc tcatgaaggg caacaccatc gtaatggaga
     2161 ttgttcatgg gcctatttta tcgaaatcat acgtattatc ttctttggaa acccgggtgt
     2221 aactcaatgg tttcgtgttt tagcagattt acaggaattg tcatgtgatg aagtattggt
     2281 tggacatcaa aaaatttcgg cacatgacta tggtcactgt ctattgcaag tggtagaggc
     2341 agtatcccaa tgctctctgg catctaaccg ggaaattgca tgtacggtgg gtatggcttt
     2401 gaacaaagaa aacgaagact gcaaatttat aataaggaga atttcaatgc taactgctta
     2461 ttcacccaat gcatccagac ctttgctatt agggatagtc tttgcaggat tttctatcct
     2521 gtcaccaatc tgcgttgcct attcagcagc aggatctttg acgagttcca tggtaaaaga
     2581 agttgatatt tctcatttag atcccagaat acagcagatt gcagagaaac aaattatgac
     2641 agccgtcaaa caatatcatg caaaatctgg agttattgca atagctgatc cccaaacagg
     2701 caatatcatt gcatttgctg aatcaagtaa aaataagggg ttagagagtt ggaaatcacg
     2761 tattttttca cccggatcta ctataaaacc ttttattgca gcagctgcaa tcaactctgg
     2821 gaatagctct gaaacaaaaa attatgattg tcattcacct tatttcatag caggcaaaac
     2881 atttacaaat tatagttcca atgttgaatc agcttcctta gcagaagcta tcgcaaaatc
     2941 tatcaatgta tgccttatca gagtttctca agaggctgga gtgccggtaa tccgtaaaaa
     3001 gctcactgaa tttggttttg acacaagcac ttggtggcaa gcggatcgaa gtgatggttt
     3061 acaactggca atggctgcac ttggggagaa tatccctgtc acaatcgaat cgctaataaa
     3121 atcttatgcc attcttgcta acaaaggaca ctcatttgac agaggaaata gtgccattat
     3181 ttcagaaaca acgaattact caataaatca tatgcttgaa aatgctgtta caaatggcac
     3241 tggaaaactc gcagttattt ctggtgtttc tgtagctggt aagacaggaa ctgtaattga
     3301 aaataatgat aaatatcttg ctttgtttgc cgggtatgtg ccagctgata atccgcgtta
     3361 tgttgtatta gtcgtgattg aagagggtca tttcagtaaa aatggaaaga cattagtttc
     3421 gggaggagaa ttggccgcac cggtattccg aaatgtcgca atggatgctc tgagcagtgt
     3481 aaatcaataa ctagcttatg ggtttgcata tccatatatt caagaatgtt gaggttgtta
     3541 tgaagaaaat aattatattt ttgtacacag gattattatt ttgcgtctct acttgggctc
     3601 aagacacctg ttttttagca aaagaaaatc aaactgtttt aaaacgtgag ggcaatgatt
     3661 gtgatcaacg ctattcgcca gcatcaactt ttaaaattgc ccttagcctc atgggctttg
     3721 attctggaat attaaaggat gagttgcatc ctgaatggcc atacaaaaaa gaatatgaac
     3781 tttatctgaa tgtatggaag taccctcaaa acccgcatac ctggatcaga gactcctgtg
     3841 tttggtattc acaggcatta actcgccaat tgggcatgaa acggtttaaa gggtatgtcg
     3901 acgcatttca ctacggtaat caggatgttt cgggagacaa aggtcaaaat aacggtttaa
     3961 cacatgcctg gctttcaagc tcactttcta tctcacccac agagcaaatt caatttctac
     4021 aaaaaataat atataagaaa ctaccggtga gtcaaaaagc ttataccatg actaaaaaca
     4081 tcatgtacat ccaggagttg cctggtgggt ggaagcttta tggaaaaaca ggaactggtc
     4141 gacagttaac aaaagataaa agtcaaaaat tgcctttaca acacggatgg ttcgtaggtt
     4201 ggattgaaaa ggatgagcgt gtgattacct ttgcaaaaca tattgcagac agtaaagaga
     4261 ataccacttt tgccagtttt agagcgaaaa atgatacatt gattcaattg tttaacctga
     4321 ttaatgaact ggagaaataa tctaaaagat ggccggttca gtgttttctg atccggctat
     4381 ctaactctct gaattataat cactggttgt taatttcgca ttttagtttg gatgcttgtt
     4441 tattaaccca gattaaaaat ttaatgagat cattaatttc ttccgttgag aggtttttgc
     4501 ctatcggtgg catgtatcct cgcccattct ttacaagttt actgatatct gaaggcgtta
     4561 aataactcat caccatacct aagtctggtg cgcgtccttg agcccccttg taaaaaagag
     4621 agtggcatgt ggagcatata ttattaaaaa tatgatgacc attttgcaca gaagcagatt
     4681 cattcaatgg atttaaaaaa tgagaaaaag tcagctcttt cttatttact atttgcatcg
     4741 aaataacctg gtatggccaa ctcacagggt aggtacttgt attatcccat acaatataga
     4801 aaggaccagg attttctttt ttaccgtgtt gattaatgta actccagcga ccatctttgg
     4861 tgataatatt gtcagagatt gttccgggcg tttcttgata agcaagatag gcatgtccct
     4921 ttgtcgtaaa atacaacaag ggagtttgag acacatattt atctattgtt tgagctaata
     4981 atacctggtt tgcattattt ttttgtaaat ctgctcccat caactgctct aaaatctgat
     5041 tcatatcaaa cgcatcgtat gtttttttct gataattata tgcaggatta tgagagataa
     5101 tcacacgaat ggttttaaat tgttttttta gctccgacag ggtatatgtt ttattggcaa
     5161 tagtaatttt aagtagtttt tctggtgaat tgggt
//
LOCUS       DAXLDT010000423         1250 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000431
            SAMN39332595-rid20709773.guided.336, whole genome shotgun sequence.
ACCESSION   DAXLDT010000423 DAXLDT010000000
VERSION     DAXLDT010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39332595
            Sequence Read Archive: SRR27481074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002050333.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002050333.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 08:56:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,313
            CDSs (total)                      :: 3,263
            Genes (coding)                    :: 3,233
            CDSs (with protein)               :: 3,233
            Genes (RNA)                       :: 50
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 14 of 30
            Pseudo Genes (incomplete)         :: 16 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 5 of 30
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39332595-rid20709773.guided.336"
                     /isolate="LP000431"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-05"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            50..1048
                     /gene="aph(9)-Ia"
                     /locus_tag="ACF2SP_003313"
     CDS             50..1048
                     /gene="aph(9)-Ia"
                     /locus_tag="ACF2SP_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HFD2361050.1"
                     /translation="MLRNNIPDQHLIALLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQVDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREIVRSFYSQ
                     LGFDERDDQITTDFKAFFNQNIDSIHQLVNSSGELSKKIQPDFEKYVLCHSDVHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGSGIGNVWNKPHEINYFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHDLLSRDQNAQSRLESFKHFKSMFTPNDVVEIAFATGLI"
BASE COUNT          423 a          205 c          207 g          415 t
ORIGIN      
        1 tattatttgc tttaccatta tgataatttc caaatagtaa ttaactctta tgctaagaaa
       61 taacattcca gatcaacacc tcattgcgct tttgaaagtc tattatggaa ttgatattca
      121 tacagtacaa ctcatcgtgg gtggtgctga tatgaatgcc tttggatata aagcagattc
      181 agaatccaat tcttatttcg taaagctaaa atacggccat catgatgaaa ttaatttatc
      241 aatcatccgt ctcttacatg actctggagt aaaggaaatt gtttttccta tcaacacact
      301 ggaagcaaac ttatttcagc aagtggatca tgttaaaata attgtgtatc catttattga
      361 tgcgcccaat ggcttcacca aaaatttaac agaaaaacag tggaaacagc ttgggaaaat
      421 attaagacaa atccatggaa cctctgttcc cacctcgatt caacagcgat taagaaaaga
      481 aacttactca cctaaatggc gtgaaatcgt taggtctttt tacagtcaac ttggatttga
      541 tgagcgtgat gatcaaataa ccactgactt caaagctttt tttaatcaaa atatcgattc
      601 aatccatcaa ttagttaatt cttcaggaga actgtctaaa aaaattcaac ctgatttcga
      661 aaaatatgta ctttgtcatt cagacgtaca tgcaggtaat gtgttggtcg ttgatgagga
      721 atctatttac attattgatt gggatgagcc catgttcgca ccaaaagaac gtgatttgat
      781 gttcattggc agtggtattg gtaatgtatg gaataaacct catgaaatca attattttta
      841 tgaaggttat ggtaaaacaa gcatcgataa aaccattttg tcttattaca gacatgagcg
      901 aattgttgaa gatatagccg tgtatggaca tgacttgctt tcacgtgatc aaaatgctca
      961 atccagactt gaaagtttta aacattttaa atcgatgttt actccaaatg atgtagttga
     1021 aatagctttt gctacaggtt tgatttgatt tggataaaca cagaagaaaa aagatgaacc
     1081 gtttaatctc aaccctcctc tttttaattt cgtataaact ttgaattgca ttaaatcacc
     1141 tgaaattttt tatataacgc atcccaactt tagtaagaag acgacatgac tgtttgtgtt
     1201 atcgcaatga taagctcttg taaataattt taaattttat ggtttaaaat
//
